LOCUS       DASUCJ010000001         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1039290, whole genome shotgun sequence.
ACCESSION   DASUCJ010000001 DASUCJ010000000
VERSION     DASUCJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039290"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1402)
                     /locus_tag="VFU90_00005"
     CDS             complement(<1..>1402)
                     /locus_tag="VFU90_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5478175.1"
                     /translation="IDHFPGGTSAFTATLQKGVGSGTLARRAAPARAAQAPAPAQAAT
                     GSGDFFLVVYPSPSLGDGSGANKPWLQEFPDPVTKIFWQSWVELNQQTAARLGIDSGD
                     ILRIESPNGTLELPAYVYMGIQAETVAIAFGQGHTAYGRYAAGVGSNAGDLLPASFDP
                     ASGGFRWASTKVTITPPGKFTQVVTVEGSSRQYNRGIAMAMTVDELKAPPKEEHREKM
                     PGDASHEYLPGLRSPVAADAQGGIQSSIKTKEMGMYDPTNWSQMAKRRWAMTIDLARC
                     TGCQACVTACYAENNIPIVGAPYQQVHTAFLHGVPGGNILKGREMNWIRLERYWEGGD
                     DGGDFEARFVPMLCQHCGNAPCEPVCPVYATYHSPDGLNVQVYNRCVGTRYCSNNCPY
                     KVRYFNWFGYGEKDRPQYAWPDQMSWLLNPDVAVRSRGVMEKCSMCAQRIEAARIEAK
                     QRGVRIADGAIRMACE"
BASE COUNT          212 a          486 c          458 g          246 t
ORIGIN      
        1 tgctcgcacg ccatccggat cgcgccgtcg gcgatgcgca cgccgcgctg cttcgcctcg
       61 atgcgcgccg cctcgatgcg ctgcgcgcac atcgagcact tctccatcac gccgcgcgag
      121 cggacggcga cgtcgggatt cagcagccag ctcatctggt cgggccacgc gtactgcggg
      181 cgatccttct cgccgtaccc gaaccagttg aagtagcgga ccttgtacgg gcagttgttg
      241 gagcagtagc gcgtgccgac gcagcggttg tagacctgca cgttgaggcc atccggcgag
      301 tgatacgtcg cgtacaccgg acacaccggc tcgcacggtg cgttgccgca gtgctggcag
      361 agcatcggca cgaagcgcgc ctcgaagtcg ccgccgtcgt cgccgccctc ccagtaccgc
      421 tcgagacgaa tccaattcat ctcgcggcct ttgaggatgt tgccgccggg cacgccgtgc
      481 aggaacgcgg tgtgcacctg ctgatacggc gcgcccacga tgggaatgtt gttctcggcg
      541 tagcatgccg tgacgcaggc ttggcatccg gtgcaccgcg cgaggtcgat cgtcatcgcc
      601 cagcggcgct tcgccatctg gctccagttc gtggggtcgt acatccccat ttctttcgtc
      661 ttgatcgaag actggatgcc gccctgcgcg tccgcggcta caggcgaacg cagcccgggc
      721 aggtactcgt gcgacgcgtc gcccggcatc ttctcgcgat gctcttcttt cggcggcgcc
      781 ttgagctcat ccaccgtcat cgccatcgcg atgccgcggt tgtactggcg cgaggagcct
      841 tcgaccgtca cgacctgcgt gaacttaccc ggcggcgtga tcgtgacctt ggtggatgcc
      901 caacggaagc cgccggatgc gggatcgaag ctcgcgggaa ggaggtcgcc cgcgttggaa
      961 ccgacgccgg ccgcgtagcg tccatacgcc gtgtgcccct gcccgaacgc gatcgcgacc
     1021 gtctcggcct ggatccccat gtacacgtac gcgggcagct cgagcgttcc gttaggcgac
     1081 tcgatccgca aaatgtcgcc ggagtcgatg cccaaccggg cggccgtctg ctggttcaac
     1141 tcgacccagc tctgccagaa gatcttggtg acggggtcgg ggaattcctg cagccacggc
     1201 ttgttcgcgc cgctgccgtc gcctaacgac ggcgacggat acacgaccag gaagaagtcg
     1261 ccgctgccgg tcgccgcttg cgcgggagcc ggcgcctgcg cggcccgtgc cggcgcggcg
     1321 cgacgtgcga gcgtgcccga ccccacaccc ttctgcagcg tggccgtgaa cgccgacgtt
     1381 ccacccggga agtggtcgat ca
//
LOCUS       DASUCJ010000002         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1314389, whole genome shotgun sequence.
ACCESSION   DASUCJ010000002 DASUCJ010000000
VERSION     DASUCJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314389"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFU90_00010"
     CDS             complement(<1..1286)
                     /locus_tag="VFU90_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU5478176.1"
                     /translation="MTRQPRPSTNLKQYWSHGGITNQIRSEIESILASPAQVMPGAEP
                     GIVALTSLAPGAESIAVQRASIASWRAAGLDVRAFNHPSEIPELQSVYDVEFVPVAES
                     TERIFGKPYVPIHAMLQWAARDAVPVLLVNSDIELALAPSEMRRIRYIAEGGLCYFVR
                     HNHDGAPHSAVPSADGFDAFLLHGRDAALFGECFLSMGAPFWDYWLPCTFLARGLPVH
                     AVEFPAAFHRNHGVRWSWTDWHCCAAEFDRVLRSQGDARPGLAGVNSWHVRSEIERAR
                     QPLRRDPETIRDWVERTFGDGAAKTFVELGAHRGTDTSWLAATPGVTLHAFEPDPRND
                     VPAAPNVVVNRAAIAARDGRCALTQSAWGWGVEWTHSSSIRRPKLHFARYPVTFGETV
                     EVDAVALDTYWQRRGLGSIDFVWANVQGAECDLIEGG"
BASE COUNT          224 a          439 c          432 g          200 t
ORIGIN      
        1 ccgccttcga tcagatcgca ctccgcccct tgcacgttcg cccacacgaa gtcgatcgag
       61 ccgagcccgc gacgctgcca ataggtatcg agcgcgacgg cgtcgacttc gacggtctcg
      121 ccgaacgtga cgggataccg cgcgaagtga agcttcgggc ggcggatgga cgaggaatgg
      181 gtccattcga ccccccagcc ccacgcgctc tgggtcaagg cgcatcgtcc gtcgcgcgcg
      241 gcgatcgcgg cccggttcac gacgacgttc ggagccgcag gcacgtcgtt acgcgggtcg
      301 ggctcgaacg cgtgcagcgt gaccccgggg gtcgccgcca gccacgacgt atccgtacct
      361 cgatgcgccc ctagctccac gaatgtcttc gcggctccgt cgccgaaggt tcgctcgacc
      421 caatcccgga tagtctccgg gtcccggcgg agaggctgcc gcgcgcgctc gatctcgctg
      481 cggacgtgcc acgaattgac gcccgcgaga ccgggccgtg cgtccccctg gctacgaagc
      541 acacggtcga actcggccgc gcagcagtgc cagtcggtcc aactccaccg cactccgtgg
      601 tttcgatgga acgccgcggg aaattcgacg gcatggaccg ggagcccacg cgcgaggaac
      661 gtgcatggga gccaatagtc ccagaagggc gcccccatgc tgagaaagca ttcgccgaac
      721 agagcggcgt cgcgcccgtg gaggaggaag gcgtcgaagc cgtccgccga cggcaccgcg
      781 ctgtgcggcg cgccgtcgtg gttgtgccga acgaagtagc aaagaccacc ctcggcaatg
      841 tagcggatcc gtcgcatttc cgacggggcg agcgcgagct cgatgtccga gttcacgagg
      901 agcaccggga cggcgtctcg cgccgcccat tggagcatcg cgtgaatggg aacgtagggc
      961 tttccgaaga tgcgctccgt actctccgcg acgggtacga actcgacgtc gtacaccgat
     1021 tgcaactccg gaatctcgga cgggtgattg aacgcgcgca cgtcgaggcc ggcggcgcgc
     1081 catgacgcga tcgaggcgcg ctgaacggcg atgctctctg cgcccggcgc cagcgaagtc
     1141 aaagcaacga ttcccggctc ggctccgggc atgacctgcg ccggcgacgc gagaatcgac
     1201 tcgatttcgc tccggatctg gttcgtgatt ccgccgtgag accagtactg cttcaggttc
     1261 gtgctcgggc gcggctgcct tgtcacgtcg acgag
//
LOCUS       DASUCJ010000003         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_152856, whole genome shotgun sequence.
ACCESSION   DASUCJ010000003 DASUCJ010000000
VERSION     DASUCJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152856"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFU90_00015"
     CDS             complement(<1..192)
                     /locus_tag="VFU90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5478177.1"
                     /translation="MEADRLEAFSDGVIAIIITVMVLEMKVPHGVDLGSLAPVLPVFL
                     TYVLSYVYLGIYWNNHHHMF"
     gene            307..636
                     /locus_tag="VFU90_00020"
     CDS             307..636
                     /locus_tag="VFU90_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478178.1"
                     /translation="MVADPVAYDFGDDVSLGGVAGAWGAVGLPVDDFDDGFVGDAGAD
                     GSLDDGLGLDDGFVGDAGVAGFALDGDAESGVGIVTGGELLLVLVGGAVELLQPAASA
                     HAAIIMT"
     gene            825..>1593
                     /locus_tag="VFU90_00025"
     CDS             825..>1593
                     /locus_tag="VFU90_00025"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5478179.1"
                     /translation="MNPARPAVVVREVGLRDGLQSIARTLPTERKLEWIRGARDAGLR
                     EIEVGSFVPPRLLPQLADTADVVAGAAKLDGLVVSVLVPNVKGAERALACGAHSMLVP
                     LSASRAHSLANLRKTPDEVVREIAAMRAMRDAAGSACELEVGISTAFGCTMQGRVEPN
                     EVLRLLRAVLEAGVDRVGLADTVGYADPKAVRELFGRAFEVAGERLACGHFHDTRGLG
                     LANVFAAWETGVRRFDASLAGIGGCPYAPGASGNVATE"
BASE COUNT          238 a          533 c          551 g          271 t
ORIGIN      
        1 gaacatgtgg tggtgattgt tccagtaaat cccgaggtag acgtagctca gcacataggt
       61 caggaacacc ggaagtaccg gcgcgagcga tccaaggtcg accccgtggg gaaccttcat
      121 ctccagcacc atcacggtga tgatgatcgc gatgacgcca tcgctgaatg cctccagccg
      181 gtccgcttcc attgtcgccg tgaatcaccc cgccactcgt ccgtggcgag ggattctacg
      241 acgatcgacg tcggccccgg tgcccgccgt cggctgcgac gaccaccatc ggcccggcgc
      301 aggccggtgg tcgccgatcc cgtcgcttac gacttcggcg acgacgtgtc gctcggtgga
      361 gtggcaggcg cctggggtgc cgtcgggctt ccggtcgatg acttcgacga cgggttcgtc
      421 ggcgatgccg gtgccgacgg ctcgctcgac gacggactcg ggctcgacga cgggttcgtc
      481 ggcgatgccg gtgttgccgg gttcgcgctc gatggcgacg ccgagtccgg cgttggcatc
      541 gtcaccggcg gcgaattgct gctcgtgttg gtcggtggcg ccgtcgagct attgcaaccg
      601 gcggcgagcg cgcacgcggc gatcatcatg acgtaggtga gcttcatggt cgttcctcgt
      661 tggttgaacg caatcccgtg cgccagttca gcacgcgggg cccatggacg aggatagtcc
      721 cccaccacgc agcgaccatc ggacgcgccc gcaaggatct gtaggacagg accccactgc
      781 cggcctctgc gcgccattcg ttgcgaaatg gctacaatca gcgggtgaac ccggcccgtc
      841 ccgctgtcgt cgttcgcgag gtaggtcttc gcgacggcct gcagagcatc gcgcgcacgc
      901 tgccgacgga aaggaagctc gaatggatcc gcggcgcgcg ggacgccgga ttgcgcgaga
      961 tcgaggtcgg ttcgttcgtg ccgccgcggc tcctgccgca gctcgccgac accgccgacg
     1021 tcgtcgccgg cgccgcgaag ctcgacggac tcgtcgtgtc ggttctggtg cccaacgtca
     1081 agggtgccga gcgcgcgctc gcatgcggcg ctcattcgat gcttgtgccg ctctcggcga
     1141 gtcgcgcgca cagtctcgcc aatctgcgca agacgcccga cgaagtcgtg cgcgaaatcg
     1201 cggcgatgcg tgcgatgcgc gacgcagccg gatcggcctg cgagctcgaa gtcggcatca
     1261 gcaccgcgtt cggctgcacg atgcagggac gcgtcgagcc caacgaggtg cttcgactgc
     1321 tgcgcgcggt gctcgaagcc ggagtcgatc gcgtcggtct cgccgacacc gtcggctacg
     1381 ccgatccgaa ggccgtgcgc gagcttttcg gccgcgcgtt cgaggttgcc ggcgagcgcc
     1441 ttgcctgcgg gcacttccac gacacgcgcg gactcgggct cgccaacgtc ttcgccgcat
     1501 gggagacggg cgtgcgccgc ttcgacgcat cgctcgccgg catcggtggc tgtccgtacg
     1561 cgccgggcgc cagcggcaac gttgcgaccg agg
//
LOCUS       DASUCJ010000004         1446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_901759, whole genome shotgun sequence.
ACCESSION   DASUCJ010000004 DASUCJ010000000
VERSION     DASUCJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901759"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..376
                     /locus_tag="VFU90_00030"
     CDS             77..376
                     /locus_tag="VFU90_00030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5478180.1"
                     /translation="MDATQPTVVDNQAQRRFELHVDGELSVLTYRIAGDRIRLLHTEV
                     PGDQRGHGYADMLARAALERAQRDHLRVVALCPFVRAFLARHPEYNTLTLPDAPG"
     gene            complement(434..>1446)
                     /locus_tag="VFU90_00035"
     CDS             complement(434..>1446)
                     /locus_tag="VFU90_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEU5478181.1"
                     /translation="GRRCLTDNRSHQLFCLEHGGPMQLTGIHHLTAISAAIRENYRFY
                     TRTLGMRLVKRSVNQDDVTAYHLFYADARGTPGTDLTFFDWPVLRESRGSRSITRTHL
                     RVAGLPALEYWRRHLAEGGVTVGNVVERDGRLTLDFEDPEGQRLALVADGGTGDAYPW
                     ERSPVPAEFQMRGLGPILISVPNLGPTDALLQRVMGMRPVREYAHPENDAHRVHVYEM
                     GAGGPSAELHVAVQPGLRAARQGAGGVHHVAFRTPDADYDAWADRLDTLHVANSGKVD
                     RFWFHSLYFRDPSGVLFEIASDGPGFGLDEDPSTLGERVVLPPFLEPERERIVAGLQP
                     ID"
BASE COUNT          235 a          528 c          460 g          223 t
ORIGIN      
        1 gaccgcaccc cgcggtcgca ggcaatgagc cgctcgctgc ttcggtcgat cgcctcagcc
       61 tgcggtatat tcgaatatgg acgccacaca accgaccgtg gtcgataatc aggcccaacg
      121 tcgcttcgag ttgcacgtcg acggcgagct gtcggtgctc acgtaccgca tcgccggtga
      181 tcgcatccgg ctgctccata ccgaggttcc gggcgaccag cgcggacacg gctatgccga
      241 catgctcgcc cgcgccgcgc tcgagcgcgc ccaacgcgat cacctccgcg tcgtggcgct
      301 ctgtcccttc gtgcgggcgt ttctcgcgcg gcatcccgag tacaacacgc tgacccttcc
      361 ggacgcgccc ggctgatgca cgaacgcggg acgcgccggc cggcgcgccc cgcgtgtctc
      421 gagcgcggtc gattcagtcg atcggctgca ggcccgccac gatgcgttcg cgctccggct
      481 cgaggaacgg cggcagcacc acccgctcgc ctaacgtcga cggatcctcg tccagcccga
      541 accccgggcc atcggacgcg atctcgaaca gcacgcccga cggatcccgg aaatacaaac
      601 tgtggaacca gaatcgatcc actttgccgc tgttcgccac gtgcagcgtg tccagccggt
      661 ccgcccatgc atcgtagtcg gcgtccggcg tccggaatgc gacgtggtgc acgccgccgg
      721 cgccctgtcg cgccgcccgc aggccgggct gcaccgccac gtgcagctcg gccgacggac
      781 caccggcgcc catctcgtac acgtgcacgc gatgggcatc gttctcggga tgcgcatact
      841 cgcgcaccgg acgcatgccc atcacccgct gcagcagcgc gtccgtcggg cccaagttag
      901 gcacgctgat gagaatcggt ccgaggccac gcatctggaa ctccgcgggc acgggactcc
      961 gctcccacgg atacgcgtcg cccgttccac catcggcaac caacgcgagc cgctgtccct
     1021 ccggatcctc gaagtcgagg gtgagacggc cgtcgcgttc gacgacgttc cccaccgtca
     1081 cgccgccctc ggccaggtgc cggcgccagt actcgagcgc cggcagaccg gccacgcgca
     1141 gatgcgtgcg ggtgatcgac cgcgatccac gcgactcgcg cagcacgggc cagtcgaaga
     1201 acgtgaggtc cgtaccaggc gtgccgcgcg cgtcggcgta gaacaagtga tacgccgtca
     1261 cgtcatcctg gttcacgctg cgcttgacca gccgcatgcc caacgtccgg gtgtagaacc
     1321 ggtagttctc gcggatcgcc gccgagatgg ccgtgaggtg atgaatgccg gtgagttgca
     1381 tcggtccgcc gtgctcgaga cagaagagtt ggtgcgatcg gttgtcagtt aggcagcgcc
     1441 ggccgg
//
LOCUS       DASUCJ010000005         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_626692, whole genome shotgun sequence.
ACCESSION   DASUCJ010000005 DASUCJ010000000
VERSION     DASUCJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626692"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFU90_00040"
     CDS             complement(<1..667)
                     /locus_tag="VFU90_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5478182.1"
                     /translation="MRVRFVLPVVFAVAASCGRRTPASGSGAIVVYTAASVAIPIRAA
                     LDTFAAHQHVTVQQENGGSLDIARKLTELHEIPDVVVLADYDVFPKLLVPGQTTWYAE
                     FARNRMVLAFTNTSKFASQVDSTNWPQILQRPGVQVGRANPDLDPNGYRTLLTMQLAE
                     RYYDQPGLYDKLMKLSPAKNVRPREADLVGVLQAGEFDYIWSYESLALATKLRYVTLP
                     SAID"
     gene            complement(702..974)
                     /locus_tag="VFU90_00045"
     CDS             complement(702..974)
                     /locus_tag="VFU90_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5478183.1"
                     /translation="MRSSLEEIHTRGSIRAAASSLGVGYRHALAWIRRAESLVGQALV
                     ERHAGGSTGGGASLTEKAQRVTRAYRRVNASIHRLVERAEREILDS"
     gene            1130..2098
                     /locus_tag="VFU90_00050"
     CDS             1130..2098
                     /locus_tag="VFU90_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5478184.1"
                     /translation="MTVAVDIDHVSKRFANHVAVRDLSLQIPTGMVYGLLGPNGAGKT
                     TTIRMVLDVIEPDTGSIRILGSPNGQPGVVDRVGYLPEERGLYRRMTVRRVLLFLAQL
                     KGVPAAEAAKRIDAWLDRLQLITPERDWGDARVEELSRGMQQKVQFIGALVHDPVVMI
                     LDEPFSALDPINAQALKDIIIELKHAGKTVIFSTHLMESAERLCDAVCIIANGRKLVD
                     GTLADVRRAHGVQQVALALEGGSHNGVSRVLADRTLVARVDDSNQMMELDLAPKADPQ
                     HLLRALIEAGAIVQRFELVQPSLHRVFVDRVSALSSNGETTGAADA"
     gene            2091..>2741
                     /locus_tag="VFU90_00055"
     CDS             2091..>2741
                     /locus_tag="VFU90_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478185.1"
                     /translation="MRKLWIVAQREFMERVRSRWFIVSTILVPGLLGLSIFIPIFFTS
                     RAAPSPDMARVTIIDASGSGFGAGLAARLDGGVTGDTAAEYVDVVPEAIAAAESTATR
                     DVVAGRRKGYLVVGPLLWPKPDVRYAGSNATSTIDMSRLQRLVHDELIATRLRSAGMD
                     PHQVDALTALNLQMSTERLTARGRGGSGEIGSVVAFIVAFLLYITIFFYGQIVMRGV"
BASE COUNT          455 a          929 c          897 g          460 t
ORIGIN      
        1 ggtcgatcgc ggaagggagc gtgacgtacc gcagcttggt cgccagcgcg agcgattcgt
       61 acgaccagat gtaatcgaac tcgccggcct gcaacacgcc caccaaatct gcctctctgg
      121 gccgcacgtt tttcgccggt gacagcttca tcaatttgtc gtacaacccg ggttggtcat
      181 agtagcgctc cgcgagttgc atcgtgagca gcgtccgata gccgttcgga tcgagatccg
      241 gattcgcccg cccgacctgc acgccgggcc gctgtaagat ctgcggccag ttcgtgctgt
      301 ccacctgcga cgcgaacttc gacgtgttcg tgaacgcgag caccatgcgg ttgcgcgcaa
      361 actcggcgta ccacgtcgtc tgaccgggca cgagcagctt cgggaacaca tcgtagtcgg
      421 ccagcacgac gacatcggga atttcgtgca gctcggtgag cttgcgcgcg atgtcgaggc
      481 tgccgccgtt ctcctgctga acggttacgt gctggtgcgc ggcgaaggtg tcgagcgcgg
      541 cgcggatggg aatggcgacg ctcgccgcgg tgtagaccac gatcgcgccg ctgccggagg
      601 ccggggttct cctgccgcag gaggcggcca cggcgaaaac gacgggaagc acgaagcgca
      661 cgcgcacgat gactcgctcc tgccgtgaac ggaaggaaga ctcagctgtc gaggatctcg
      721 cgctcggcgc gctcgaccag gcggtggatc gacgcattga cccggcggta ggcacgcgtc
      781 acccgctgcg ccttttccgt taggctcgcg ccgccgccgg tcgacccgcc ggcgtgccgc
      841 tccaccaacg cctgtccaac gagcgactcg gcgcgcctga tccacgccag cgcgtgccga
      901 tagccgacgc ctaacgatga agcggcagcg cggatcgatc cccgggtgtg gatctcctcc
      961 aaagaggatc gcatggaggc ggcgggaggc tggcgttgtt accaaatggt aacaaccaac
     1021 gtgcagcgat ccggccgccc cggcaagggc gccggccgcc tcaggctgga accctgcgac
     1081 cccgcagccg tattgttgtg gggatgcttc ccccgcccat ggacccgcca tgaccgtcgc
     1141 tgtcgacatc gatcacgtct ccaaacgctt cgccaaccac gtcgccgtcc gcgacctctc
     1201 gctccagatt cctaccggca tggtctacgg tctcctcggc cccaacggag ccggcaagac
     1261 caccaccatt cgcatggtgc tggatgtcat cgagcccgac accggctcga tccggattct
     1321 gggttcgcct aacggacagc ccggggtcgt cgaccgcgtc ggctacctgc cggaggagcg
     1381 cggcctgtat cgccgcatga cggtgcggcg cgtgctgctg tttctggcgc agctcaaggg
     1441 cgtgccggcc gccgaagccg cgaaacgcat cgacgcgtgg ctcgaccgcc tgcagctgat
     1501 cacgcccgag cgcgactggg gcgacgcgcg cgtcgaagag ctgtcgcgcg gcatgcagca
     1561 gaaggtgcag ttcatcggcg cgctggtgca cgacccagtg gtgatgatcc tcgacgaacc
     1621 gttcagcgcc ctcgacccga tcaacgcaca agcgctcaag gacatcatca tcgagctcaa
     1681 gcacgccggc aagacggtga tcttcagcac ccacctgatg gaaagcgccg agcgactctg
     1741 cgacgccgtg tgcatcatcg ccaacggccg gaagctcgtg gacggcacgc tggccgacgt
     1801 tcgacgggcg cacggcgtgc agcaagtggc gctcgcgctg gagggcggct cgcacaacgg
     1861 cgtctcgcgc gtgctcgcgg atcgcacgct cgtcgcgcgc gtggacgact cgaaccagat
     1921 gatggagttg gatctggcgc cgaaggccga cccgcagcat ctgctgcgcg cgctgatcga
     1981 ggccggagcg atcgtccagc gattcgagct cgtccagcca tcgctgcacc gcgtgttcgt
     2041 ggaccgcgtc tctgcgctct cgtctaacgg agagaccacg ggagccgcag atgcgtaagc
     2101 tctggatcgt cgcgcagcgc gaattcatgg agcgcgtgcg gtcgcgctgg ttcatcgtga
     2161 gcacgattct cgtgcccgga cttctcgggc tctcgatttt cattccgatc ttcttcacct
     2221 cgcgcgccgc gccgtcgccg gacatggcgc gcgtcacgat catcgacgcc tcgggcagcg
     2281 ggttcggcgc cggtctggcg gcgcgcctcg acggtggcgt caccggcgac accgccgccg
     2341 agtacgtcga cgtagttccg gaggcaatcg cggcggcgga atccacagcc acacgcgacg
     2401 tggtcgccgg gcggcgcaaa ggctacctgg tggtcggtcc gctcttgtgg ccaaagccgg
     2461 acgtgcgcta tgcgggttcg aacgcgacgt cgacgatcga catgtcccgc cttcagcgcc
     2521 tggtgcacga cgagctgatc gcaacccggc ttcgttccgc ggggatggac ccgcaccagg
     2581 tcgatgcact cacggccttg aacctccaga tgtccaccga acgcctaacg gcgcgcggcc
     2641 gcggcggatc cggcgagatc ggcagcgtcg tcgcgttcat cgtggcgttt ctgctctaca
     2701 tcacgatctt tttctacggc cagatcgtca tgcgcggcgt g
//
LOCUS       DASUCJ010000006         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_351574, whole genome shotgun sequence.
ACCESSION   DASUCJ010000006 DASUCJ010000000
VERSION     DASUCJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351574"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="fabD"
                     /locus_tag="VFU90_00060"
     CDS             complement(<1..785)
                     /gene="fabD"
                     /locus_tag="VFU90_00060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5478186.1"
                     /translation="MRVGVMFPGQGSQGVGMGTDVAARSPEAAALLRRASSVLGYDLM
                     ALLKGGPEEKLRETRYSQPAIFATNLALYAAAGDGLQPVVSAGHSFGEFCSLVAARSL
                     DFDAALRIVDERGKAMQAAAELAPGGMSAILGLDAVQVRRIVEATQADGGGRVTLANF
                     NSPTQIVISGDLAAVQTAGEAMLGAGAKRVVPLNVSGAWHSELMEPAVERFRAAVEAA
                     HFDLPAFDVISNVDARPYRDVATIKLNLVRSLAHEVRWHDTAER"
     gene            complement(785..1774)
                     /locus_tag="VFU90_00065"
     CDS             complement(785..1774)
                     /locus_tag="VFU90_00065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5478187.1"
                     /translation="MSLRGVKIVGVGHHAPAHVVTNGDLEQWLDTSDEWITTRTGMKR
                     RHWASSGEATSDLAFAAAREALDYAGLQPRDIDCFIVCTVTPDYYFPATACLVAARLG
                     AKERPAFDISIACSGFIYGLTVASGLVRAGIYRRVMLIGAETLSKILNKEDRATAILF
                     GDGAGAVVLEASDENSFLSSELGADASRPEMLYVRSSGSRHPIDRAALDERMNLIQMQ
                     GREVFKSAVTKMIEATDKALLKAKLAKSDVAFLIPHQANKRIIDAAAHYLEMPADKVV
                     TNIHEYGNTSAASIPMALSESVRAGMIEPGDVIVFVAFGGGLSWGAVAWKWAA"
     gene            complement(1790..1996)
                     /gene="rpmF"
                     /locus_tag="VFU90_00070"
     CDS             complement(1790..1996)
                     /gene="rpmF"
                     /locus_tag="VFU90_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5478188.1"
                     /translation="MANLKWKTPRSKTRSRRAANWKLGTVTTVECPQCHQPKRPHFAC
                     ESCGTYAGRQVVEVRDEHTGHDHA"
     gene            complement(1962..2462)
                     /locus_tag="VFU90_00075"
     CDS             complement(1962..2462)
                     /locus_tag="VFU90_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5478189.1"
                     /translation="MARSHKVDIVGLLAGSRQLMLVDDEVPIEPFEGIAFPEPARVHL
                     ELRSADRMLVAEGSVDAVACGRCDSCLEDVQRKVHVDVDERFDPSTEREEDPFGEGNV
                     LTGGRLDVADLAQQLVLSVLPLGMRCTDDCLGLCDTCGANRNTGECSCLNGDIRGKPK
                     MEDAAQ"
     gene            2589..>2863
                     /locus_tag="VFU90_00080"
     CDS             2589..>2863
                     /locus_tag="VFU90_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478190.1"
                     /translation="MNVDPIASRLAAQGVAYAPQIAGAARRHGLDPELLAAVAAQETG
                     GPGANAGRNVVGDGGHGHGLFQIDDRWHAFARSPEAMDPGRNAEYAAG"
BASE COUNT          479 a          996 c          894 g          494 t
ORIGIN      
        1 cgctcggccg tatcgtgcca gcgtacttcg tgcgccagcg agcgcaccag attcaatttg
       61 atcgtcgcca cgtcgcggta ggggcgcgcg tcgacgttcg agatcacgtc gaacgccggc
      121 aggtcgaaat gcgctgcttc gaccgccgcg cgaaaccgct cgacggccgg ttccatcagc
      181 tcgctgtgcc aggctcccga aacgttcagc ggaaccacgc gcttggcgcc cgcgccgagc
      241 atcgcctcgc cggccgtctg aacggccgcg aggtcgccgc tgatgacgat ttgggtcggc
      301 gagttgaagt tcgcaagcgt cacgcggccg ccgccgtccg cttgagtcgc ctccacgatc
      361 cgccgcacct ggacggcatc taaaccgaga atcgccgaca tgccgccggg tgcgagctcg
      421 gcggcggctt gcatcgcttt cccgcgctcg tcgacgatcc gcagcgcggc atcgaaatcc
      481 agcgaccggg ccgccaccaa actgcaaaac tcaccgaagg agtgtccggc gctgaccacc
      541 ggctgcagcc cgtcgcccgc ggccgcgtag agcgccaagt tcgtcgcgaa aatcgccggc
      601 tggctgtagc gggtttcgcg gagtttttct tcggggccgc ccttcagcag cgccatcaaa
      661 tcgtatccga gtaccgatga ggcccgccgc agcagcgccg cggcttcggg cgaccgcgcg
      721 gctacgtcgg tgcccatgcc gacgccctgc gagccctgcc ccggaaacat tacgccgacg
      781 cgcatcacgc ggcccacttc catgcgaccg cgccccacga caacccaccg ccgaacgcga
      841 cgaagacgat cacgtcgccg ggttcgatca tccccgctcg caccgattcg gacaacgcca
      901 tcgggatcga cgccgccgac gtgttgccat attcgtggat gttcgtcacg actttgtccg
      961 cgggcatttc gagatagtgc gccgcggcat cgataatccg cttgttcgcc tgatgcggga
     1021 tgaggaaggc gacgtcgctc ttagcgagct tcgctttgag caacgctttg tcggtcgctt
     1081 cgatcatctt ggtaacggcc gacttgaaga cttcccgccc ctgcatctga atcaggttca
     1141 tccgctcgtc gagcgcggca cggtcgattg ggtggcgcga accgctcgag cgaacgtaga
     1201 gcatttccgg gcggctggcg tcggcgccca gctccgaaga cagaaacgag ttttcgtcag
     1261 acgcttccag caccacggcg cccgcgccgt cgccgaagag gatcgccgtg gcgcgatctt
     1321 ctttgttgag gattttcgaa agggtctccg ctccgataag catcacgcgg cggtaaatgc
     1381 cggcgcggac gaggccggat gcgaccgtca agccgtagat gaacccgctg caggcgatcg
     1441 agatgtcgaa ggccggccgt tccttggccc ctaggcgggc cgccaccaaa cacgcggtgg
     1501 cggggaagta gtaatccggc gtgacggtgc agacgataaa acagtcgatg tcgcggggtt
     1561 gtagtccggc gtagtccagt gcctcacgcg ccgcggcgaa cgccaaatcg ctggtcgcct
     1621 cgccggacga cgcccaatgc cggcgcttca ttccggtgcg cgtcgtaatc cattcgtccg
     1681 atgtgtccag ccactgctcc aagtcgccgt tggtgacgac gtgcgcgggc gcgtgatgcc
     1741 cgacgccgac gatcttcacg ccgcgcagcg acatacgggt tcaggaccct tacgcgtggt
     1801 cgtgaccggt gtgttcgtcc cggacttcga cgacctgtcg tcccgcgtac gtgccgcagc
     1861 tctcgcaagc gaaatgcggc cgcttcggct gatggcactg cggacactcc acggtggtca
     1921 ccgtgccgag tttccagttt gccgctcgac ggctgcgcgt tttactgcgc ggcgtcttcc
     1981 attttaggtt tgccacgtat gtctccgttt aggcacgagc actcgcccgt atttctgttt
     2041 gctccgcaag tatcgcacag gcctaggcaa tcgtcggtgc agcgcatacc cagcggaagg
     2101 acgctgagca ccagttgctg cgccagatcg gcaacgtcga gccggccgcc ggtcagcacg
     2161 ttgccttccc cgaacggatc ctcctcgcgt tccgtggacg ggtcgaaacg ttcgtcgacg
     2221 tcgacgtgaa ccttccgctg tacgtcctcc aagcacgagt cgcaccggcc gcacgccacg
     2281 gcgtcgacgc tcccctcggc cacgagcatc cggtccgcgc tgcgcagctc caaatgcacg
     2341 cgtgccggtt cgggaaacgc aatcccctcg aacggttcga tcggcacctc gtcgtccacc
     2401 agcatgagct ggcggctccc ggccaggaga cctacgatat cgaccttgtg cgagcgagcc
     2461 atgccaccaa cgcgactgca gtatgcgggg gtcccgccgc ccggcgagac ccccacaaca
     2521 acggagtcat tctaagaccc ccaccgctcc ttgtcaaacg ccctcgtctg agggaagaga
     2581 cgcttgctat gaacgtcgac ccgattgctt cgcgtcttgc ggcccagggg gtcgcgtatg
     2641 caccccaaat cgccggcgcc gcccggcgcc acggcctcga tccggaactg ctggccgcgg
     2701 tggcggctca agagaccggc ggccccggcg ccaacgccgg ccgtaacgtg gtgggcgacg
     2761 gcggccacgg ccacgggctg tttcagatcg acgatcgctg gcacgcgttc gcccgctcgc
     2821 ccgaggcgat ggaccccggc cggaacgcgg agtacgccgc ggg
//
LOCUS       DASUCJ010000007         1447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1115756, whole genome shotgun sequence.
ACCESSION   DASUCJ010000007 DASUCJ010000000
VERSION     DASUCJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115756"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFU90_00085"
     CDS             complement(<1..1075)
                     /locus_tag="VFU90_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478191.1"
                     /translation="MRVRDYAVIAAIAAATACGSSTTQPNRSTTPCPSGAVKTTATLD
                     VGGVQAVANPTALDCVALATTNGQSGTFLVVAGNADTDPDVMGSYDLAVTAPTSADVA
                     AGDRLRAAALQSPAEGGMYSSIAEGRIRAMERRVLLENAGAMRAASAFLRRSTGRVRA
                     ESVPNVGDTLTLKVPNANATNACLTFTTQRAVVKTVGTHGIIVQDVTAPTGGFSAAAF
                     DSIETEFDQIIYPADTNHFGSPSDVDNNGHVYLFFTPQVNAATKRGSNSILEGFFFIG
                     DLFQVSQCAESNQSELFYLLTPDPTGKFSDVRSTSSVRQDIRGTIAHEFQHMINAGVR
                     LSTGAPDEATWLNEGLSHFAEELV"
     gene            1237..>1447
                     /locus_tag="VFU90_00090"
     CDS             1237..>1447
                     /locus_tag="VFU90_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEU5478192.1"
                     /translation="MHRHAITHLRRDPVLARVMADVGPCRFAPRSDGSHFDHVLRAIV
                     YQQLSGRAAATIHSRVVALFDGKPSA"
BASE COUNT          225 a          515 c          448 g          259 t
ORIGIN      
        1 ccaccagctc ctcggcaaag tgcgacaatc cttcgttgag ccacgtagcc tcgtccggcg
       61 cgcccgtgct caaccggacg cccgcgttga tcatgtgctg gaactcgtgg gcgatcgttc
      121 cgcgaatgtc ctggcgcacc gacgacgtcg accgcacgtc gctgaatttg ccggtcgggt
      181 cgggcgtcaa cagatagaac aactccgact ggttgctctc ggcgcactgg ctgacctgga
      241 acaggtcgcc gatgaagaag aacccctcga ggatgctgtt cgagccgcgc ttcgtcgccg
      301 cgttcacttg cggcgtgaag aacaggtaca cgtgcccgtt gttgtcgacg tccgacggac
      361 tgccgaagtg gttggtgtcc gccggataga tgatctgatc gaactcggtc tcgatcgaat
      421 cgaacgccgc cgcgctgaat ccgcccgtcg gcgccgtgac gtcctgcacg atgatgccgt
      481 gcgtgccgac cgtcttcacc acggcgcgct gcgtcgtgaa cgtcaggcac gcgttcgtgg
      541 cgttcgcgtt gggcaccttc aaggtcaacg tatcgccaac gttaggcacg ctctccgcac
      601 gcacgcggcc cgtgctccgg cgcaggaacg cggacgccgc ccgcatcgcg ccggcgttct
      661 ccagcagcac gcgccgttcc atcgcgcgga tccggccttc ggcgatcgag ctgtacatgc
      721 cgccctccgc cggcgattgg agcgccgcgg cgcgcaggcg atcgccggcc gccacgtccg
      781 cggacgtcgg cgccgtcacc gccaggtcgt acgatcccat cacatccgga tccgtgtccg
      841 cgttgccggc cacgaccagg aacgtgcccg attggccgtt cgtcgtggct aacgcgacgc
      901 agtcgagcgc cgtcgggttc gcgaccgcct gcacgccgcc cacgtccagg gtcgccgtcg
      961 tcttgaccgc cccgctcgga cacggcgtcg tgctacgatt cggctgcgtg gtcgaggagc
     1021 cgcacgcggt ggcggcggca atcgcggcga tcaccgcgta gtctctcact cgcatgtgcg
     1081 gatcccaggt tcgtgtggtg gtgcgcccga catgatgata gcaggcgcat gcatatttcg
     1141 aatctagctg actagccccg atcgcgccgc gaagttccgt gtgacacgac tgcgcgcctc
     1201 gacctggatg tccaacgagg aataaattct gtgcccatgc accgccacgc tatcacccac
     1261 ctgcggcgcg atcccgtgct cgcgcgcgtc atggccgacg tcggcccctg tcgcttcgct
     1321 ccccgcagcg acggatcgca cttcgaccac gtcctgcgcg ccatcgtgta tcaacaactg
     1381 tcgggacgag cggccgcgac cattcacagt cgcgtcgtcg cactcttcga tggcaaaccg
     1441 tccgccc
//
LOCUS       DASUCJ010000008         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_229347, whole genome shotgun sequence.
ACCESSION   DASUCJ010000008 DASUCJ010000000
VERSION     DASUCJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229347"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFU90_00095"
     CDS             <1..223
                     /locus_tag="VFU90_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478193.1"
                     /translation="TIEAANDYGRSGEHRPGGWFVAAGPALPTGRLHREPSLLDLAPT
                     FAALVGVALADATGAPITEIVGGSAIDGA"
     gene            388..>1836
                     /locus_tag="VFU90_00100"
     CDS             388..>1836
                     /locus_tag="VFU90_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5478194.1"
                     /translation="MPSHFLPNALVRALVAGALAASVAPVGRALAQTPGLPSETPAKF
                     KPATSTFDYERRDVMIPMRDGVKLHTVILVPKGAHRAPILLTRTPYDATATTSYAQSA
                     HLAPELNGYDNALQVIIAGGYIRVVQDIRGKYGSEGDYVMNRPLVGPLNPTTVDESTD
                     TYDTIDWLIKHVPETNGNVGELGISYDGFEPLMGLVHPHPALKVSVPMNPMVDGWMGD
                     DWFHNGAFREQNMPYILEQEATRDNSAKWWTDHFDDYDVYMQSGSAGELGKLHGLEQV
                     GFWNKILAHPSYDSWWQQQAMDKILAAQPISVPVMLVHSLWDQEDIYGAIAVYKAIEP
                     KDASNDKVFLVIGPWHHGQEIENGSSLGAVDFQANSSEYFQRNILGPFLAHYLKDGAP
                     PMDVAPVNAYETGTNRWDRLRAWPSGCDTGCAIRPTPLYLGAGLTASFDAPASSTPDA
                     AFDSYISDPSKPVPFRTRPIQPVGYTPPLTWVQ"
BASE COUNT          312 a          665 c          569 g          290 t
ORIGIN      
        1 taccatcgag gcggcgaacg actacggacg aagcggggag caccggccgg gcggatggtt
       61 cgtcgctgcc gggcccgccc tgcccaccgg acgcttgcac cgcgagccat cgctgctcga
      121 cctggcgccc accttcgccg cgctcgtagg cgtcgcactg gccgatgcca cgggcgctcc
      181 gatcaccgaa atcgtcgggg gaagcgcgat cgacggcgcg tgatccattc ccggttgggc
      241 cggtcgtctt gcgcctgcgc gttccccggt gccagtttcg gctgcgttcg tcgcgttgct
      301 ctcgcgcgtc gcgccccggc tcgctcgcgg tattctcgac gcgcgcggcg tcctgcgcgc
      361 gcccgcacac ttcccgagga ccccatcgtg ccatcgcact tcctgcctaa cgcgctcgtc
      421 cgcgccctgg tcgccggcgc gctcgcggca tccgtcgcgc ccgtcgggcg cgccctcgcg
      481 cagacgccgg gtctccccag cgagaccccg gccaagttca agcccgcgac gtcgaccttc
      541 gactacgagc gccgcgacgt gatgatcccg atgcgcgacg gcgtgaagct gcacacggtg
      601 atcctcgtgc ccaaaggcgc gcaccgcgcg ccgatcctgc tcacgcgcac gccgtacgac
      661 gcgaccgcaa cgacgtcgta cgcccagagc gcgcacctcg caccggagct caacggctac
      721 gacaacgcgc tccaggtgat catcgccggc ggctacatcc gcgtcgtcca ggacatccgc
      781 ggcaagtacg gctccgaggg cgattacgtg atgaaccgcc cgttagtcgg cccgctcaac
      841 ccgaccaccg tcgatgagtc gaccgatacc tatgacacca tcgattggct catcaagcac
      901 gtccccgaga ccaacggcaa cgtcggcgag ctcggcattt cctacgatgg gttcgagccg
      961 cttatggggc tggtgcaccc gcatcccgcg ctcaaggtgt cggtccccat gaatccgatg
     1021 gtggacggct ggatgggaga cgactggttc cacaacggcg cgttccgcga gcagaacatg
     1081 ccgtacatcc tcgagcagga agcgacgcgc gacaacagcg ccaaatggtg gaccgaccac
     1141 ttcgacgact acgacgtcta catgcaatcg ggctcggccg gcgagttagg caaactgcac
     1201 ggcctcgagc aagtcgggtt ctggaacaag atcctcgcgc acccgagcta cgactcgtgg
     1261 tggcagcagc aggcgatgga caagattctc gccgcccaac caatcagcgt tccggtgatg
     1321 ctcgtccaca gcctctggga tcaggaagac atctacggcg cgatcgccgt gtacaaggcg
     1381 atcgagccca aggacgcgtc aaacgacaag gtctttctcg tcatcggccc gtggcaccat
     1441 gggcaggaga tcgagaacgg cagttcgtta ggcgcggtgg atttccaggc gaacagctcg
     1501 gagtacttcc agcgcaacat tctggggccg ttcctcgcgc actatctgaa ggacggcgcg
     1561 ccgccgatgg atgtggcgcc cgtgaacgcc tacgagacgg gcaccaaccg ttgggaccga
     1621 ctgcgcgcgt ggccgtcggg ctgcgacacc gggtgcgcca tccgcccgac gccgctctat
     1681 ctcggtgctg gcctaacggc aagcttcgat gcgcccgcgt cgtcgacgcc cgacgccgcg
     1741 ttcgactcgt acatctccga tccctccaag cctgtcccgt tccgcacgcg acccatccag
     1801 ccggtcggct acacgccgcc gctcacctgg gtgcag
//
LOCUS       DASUCJ010000009         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1329740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000009 DASUCJ010000000
VERSION     DASUCJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2157)
                     /locus_tag="VFU90_00105"
     CDS             complement(<1..>2157)
                     /locus_tag="VFU90_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5478195.1"
                     /translation="PAAAAAPPPIPPGANLVPLRGPAAALAGYMEQSLTIPTATSFRS
                     VPVDVLDARRKELNGAIKAAGRSEKISFTHVVAYALVRAAADLPFITFSFRRDERGAP
                     ARLEPGIHLGLAVDSARKDGTRSLVVPVIRNADSLDFAAFRATYEELVAKARDNKLAA
                     DDLQGASFTLTNPGGIGTIASVPRLMAGQGAILATGAIGYPPGFAGANEQSLQLLGVS
                     KIMQVTSTYDHRVIQGAQSGEYLRHVDELLQGRGGFYESIFESLGLQAAAVPHVTTAV
                     AAAGGARAAAPPSDEMLRAVASGMAIVSAYRRYGHLAANLDPLGTQPVGDPSLEPQSY
                     GLTPALQAAIPAGVLRVKVPGNTLGEVLPHLKETYSSTIAYEIEHISNATQRTWLRDY
                     IESGKNKIKFSPERQLEFLQRLTRVEAFDRYVRKAFLGQKTFSGEGLDVMVPMLEEML
                     DMFADDGIANAVIGMAHRGRLNVIAHVVNMPYEEVMTEFEAAQYRGELGDDDVMGDVK
                     YHHGATGTFVTAKGKSIGVTLAHNPSHLEAVDPVVEGSARALQTDHAHGVPSYSLERA
                     APILIHGDAAFTGQGIVSEVFNLQSLPGYQTGGTVHLIANNQIGFTTDNADARSTRYA
                     SDLAKGFDVPIVHVNADDVDACIAAVHLAVDFRRAFDRDVLIDLIGYRRFGHNEQDEP
                     AYTQPQMAEKIKGHPTVRELFANKLVNQGILTAEQA"
BASE COUNT          344 a          703 c          735 g          375 t
ORIGIN      
        1 gcgcctgttc ggccgtcaaa attccctgat tgacgagctt gttggcgaac agctcgcgaa
       61 ccgtcggatg gcccttgatc ttctcggcca tctgcggctg ggtgtaggcg ggctcgtcct
      121 gttcgttgtg accgaaccgg cggtagccga tcagatcgat cagcacgtcg cgatcgaagg
      181 cgcggcggaa gtcgaccgcc aggtgcaccg ccgcgatgca ggcgtcgacg tcgtcggcgt
      241 tgacgtgcac gatcggcacg tcgaagcctt ttgccaggtc cgaagcgtag cgcgtcgagc
      301 gcgcgtcggc gttatcggtg gtgaagccga tctgattgtt ggcaatcagg tgaaccgtgc
      361 cgccggtctg atacccgggc agcgattgaa ggttgaaaac ctccgagacg attccctgac
      421 cggtaaaggc ggcgtcgccg tgaatcagaa tcggagccgc gcgttcgagc gaataggacg
      481 gaaccccgtg cgcgtggtcg gtctgcagcg cgcgcgcgct gccctcgacg accgggtcga
      541 cggcttccag atggctggga ttgtgcgcca gcgtgacgcc gatcgacttg cctttggcgg
      601 tcacgaacgt gccggttgcc ccgtgatggt acttgacgtc gcccatcacg tcgtcgtcgc
      661 cgagctcgcc gcgatactgc gccgcttcga actccgtcat cacctcttcg tacggcatgt
      721 tcacgacgtg ggcgataacg ttgagccggc cgcggtgcgc cataccgatg acggcgttgg
      781 cgatcccatc gtcggcgaac atgtcgagca tctcctcgag catcggcacc atcacgtcca
      841 ggccttcgcc cgagaacgtt ttctggccga ggaacgcctt gcgcacgtag cggtcgaacg
      901 cctcgacgcg cgtcagccgc tggagaaact ccagctgccg ctccggcgag aatttgatct
      961 tgttcttgcc cgactcgatg tagtcgcgca gccacgtgcg ctgggtggcg ttggaaatgt
     1021 gctcgatctc gtacgcgatc gtcgaactgt aggtttcctt gagatgcggg agcacctcgc
     1081 ccagcgtgtt gccgggaacc ttgacgcgca gcacgccggc cggaatggcc gcctgcagcg
     1141 cgggcgtcag accgtagctc tgcggctcga gcgacggatc gcccaccggt tgcgtgccga
     1201 gcggatccag gtttgcggcg aggtgaccgt agcgccggta ggccgacacg atcgccatgc
     1261 cggaagctac cgcgcgcagc atctcgtccg acggcggcgc ggccgcacgc gctcctcccg
     1321 cggcggcgac ggcggtcgtg acgtgcggca cggcggcggc ctgcagcccc agcgactcga
     1381 agatcgattc gtagaagccg ccgcgtccct gcagcagctc gtcgacgtga cgcaagtact
     1441 cgcccgactg cgcgccttgg atgacgcgat ggtcgtacgt gctcgttacc tgcatgattt
     1501 tcgaaacgcc cagcagctgg agcgactgct cgttggcgcc cgcgaagccg ggcgggtagc
     1561 cgatcgcccc ggtcgccagg atcgcgcctt gaccggccat cagccgggga accgacgcga
     1621 tcgttccgat cccgcccggg ttggtcagcg tgaacgaggc gccttgcaga tcgtcggcgg
     1681 cgagcttgtt gtcgcgcgct ttcgcgacca gctcctcgta cgtcgcgcgg aacgcggcga
     1741 aatcgagcga atcggcgttc ctgatcaccg gtacgactag ggatcgcgtg ccgtctttgc
     1801 gcgcgctgtc gaccgccagc ccgagatgga tgcccggctc gagacgcgcg ggcgcgccgc
     1861 gctcgtcgcg acgaaacgaa aacgtaatga acggcagatc cgcggcggcg cgcaccagcg
     1921 cgtaggcgac gacgtgggta aacgagatct tctcgctacg cccggccgcc ttgatcgcgc
     1981 cgttgagctc tttgcggcga gcgtcgagca cgtcgaccgg aacggaacga aaactcgtcg
     2041 ccgtcggaat cgtgaggctc tgctccatgt agccggcgag cgcggccgcg ggtccgcgca
     2101 gcggcaccag gttcgcaccc ggcggaatcg gcggcggcgc ggctgcggca gccggcg
//
LOCUS       DASUCJ010000010         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1100477, whole genome shotgun sequence.
ACCESSION   DASUCJ010000010 DASUCJ010000000
VERSION     DASUCJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..>1156)
                     /locus_tag="VFU90_00110"
     CDS             complement(161..>1156)
                     /locus_tag="VFU90_00110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5478196.1"
                     /translation="ETLEAKLPGYEIAIQSATDDETRMIVAAFNDRTQGMRYVYDRAA
                     DRLTPLGAVAPWLPEADMAAVRPISYRTRDGLVIHGYLTLPNGAPPKGLPVVVNPHGG
                     PWYRDGWGFNPELQFLANRGYAVLQMNFRGSTGYGRTFWEASFKEWGGTMQDDITDGV
                     RWLIAEGIADPRRICIYGGSYGGYAVLAGLTKTPELYACGIDYVGVSNLFTFMQTIPP
                     YWKPYLTMMYEMVGDPERDRVLLTERSPALNTERIRAPLLIAQGARDPRVNKDESDQM
                     VAALKARGIDVPYIVKDNEGHGFHNEENRFEFYDAMETFLARHLGAADQPAPAGS"
BASE COUNT          185 a          385 c          374 g          212 t
ORIGIN      
        1 caccggcttg ccgccgaggt cggcgtactg cttagggatg ccggcagcga agcgagtgcc
       61 gcttccggct gcggcaacga cgatccaggt ccgcgttcgt gttgccgtcc gcgtgctgcg
      121 cccgatgccc atcgctgcag ccggcgagcg ccgtcagggc ctagctaccc gccggggcgg
      181 gttggtctgc ggcgcccaga tgccgcgcga ggaaggtctc catcgcatcg taaaactcga
      241 accggttctc ctcgttgtga aagccgtgcc cctcgttgtc cttcacgatg tacggaacgt
      301 cgatgccacg cgccttcaga gctgcgacca tctggtcgga ctcgtccttg ttgacacgcg
      361 ggtcgcgtgc cccttgcgcg atcaggagcg gcgcacgaat ccgctcggtg ttgagcgccg
      421 gcgagcgctc ggtgaggagc acgcggtcgc gctcggggtc gccgaccatc tcgtacatca
      481 tcgtgaggta cggcttccag tacggcggaa tcgtctgcat gaacgtgaac aggttcgata
      541 ccccgacgta gtcgatgcca caggcgtaga gctcgggcgt cttcgtcagt ccggcgagca
      601 ccgcataacc gccgtaactg ccgccgtaga tgcaaattcg gcgcggatcg gcgatgccct
      661 cggcgatcag ccaccgcacg ccgtcggtga tgtcgtcctg catcgtgccg ccccactctt
      721 tgaacgacgc ttcccagaaa gtacgcccat agccggtcga cccccggaag ttcatctgca
      781 gcaccgcgta gccgcgattg gcgaggaact gcagttcggg attgaagccc catccgtcgc
      841 gataccacgg tccgccgtgc ggattgacga cgacgggcaa gcccttcggc ggtgcgccgt
      901 tcggaagcgt caggtagccg tggatgacga gcccgtcgcg cgtgcgatag ctgatcgggc
      961 gtaccgcggc catgtccgct tcgggcagcc acggcgccac cgcaccgagc ggcgtcagcc
     1021 gatccgcggc acggtcgtag acgtagcgca ttccctgagt gcgatcgttg aatgcagcga
     1081 cgatcatcct cgtctcgtcg tcggttgccg attgaatcgc gatctcgtag cccggaagct
     1141 tcgcttcgag cgtctc
//
LOCUS       DASUCJ010000011         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_214052, whole genome shotgun sequence.
ACCESSION   DASUCJ010000011 DASUCJ010000000
VERSION     DASUCJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214052"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFU90_00115"
     CDS             <1..477
                     /locus_tag="VFU90_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5478197.1"
                     /translation="SGMFGAIIVDPVKPLRPATELVLIQNEFYLGPPENGISPFAYTK
                     MLSELPDLLAFNGRPAQYQEAPIHVPRGKLVRFHVVNAGPTHPCAFHVVGGQFETVYL
                     GAPPNSAIHGVQTYSVAAGGGMIFEMRADEPGEYVFVNHSFGHGQKGAIGTLIVDE"
     gene            complement(544..>1225)
                     /locus_tag="VFU90_00120"
     CDS             complement(544..>1225)
                     /locus_tag="VFU90_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5478198.1"
                     /translation="FSRRRIFGHLARVIDDLAPRGAHVLDIGGARGDLMAHLVERRPD
                     LYVVVNDVSASGTKAATERFGFPTITGGAQALDEHERRYDIVVLSDVLYFEPDIRRLW
                     SALDKLVAPGGKVIIRVPHRWALIRLWQRWFRATRSRARQRLQHAIRFFNPGHIFILR
                     KDYLETRLRQLGFQRLRVMPSPLLAHAGTRVIDAVAFAVAVVIYWVTGGACALTPSVV
                     VVGADRQI"
BASE COUNT          222 a          401 c          407 g          195 t
ORIGIN      
        1 tccggcatgt tcggcgcgat catcgtcgac cccgtgaaac cgctgcgacc cgcgaccgag
       61 ctggtgctga ttcagaacga gttctacctc ggcccgccgg agaacggtat ttcgccgttt
      121 gcctatacca agatgctgag cgagctcccg gacctgctcg cgttcaatgg gcggccggcg
      181 cagtatcagg aggcgccgat ccacgttcct cgaggcaagc tggtccgctt ccatgtcgtg
      241 aacgccggcc caacccatcc ctgtgccttt cacgtcgtag gcggacagtt cgaaaccgtc
      301 tacctcggcg cgccgcctaa cagtgcgatt cacggcgtac aaacgtatag cgtcgcggcg
      361 ggtggcggca tgatcttcga gatgcgggcc gacgagccgg gggaatacgt gttcgtcaac
      421 cactccttcg gtcacggcca gaaaggcgcg atcggtacgc tgatcgtcga cgagtgaccg
      481 catccgtcga ctggacgtcg cccccgtgcg cgtcggcgtc cgggcgcgca ggcgcgtgcg
      541 gcatcagatc tgccgatccg cgccgacgac caccacactc ggcgtcaatg cacaggcgcc
      601 gccagtcacc caatagatca cgaccgcgac ggcgaaggcc accgcatcga tgacgcgcgt
      661 ccccgcgtgc gcgaggagcg gcgagggcat cacgcgcagc cgctgaaagc ccagttggcg
      721 gagacgcgtc tcgagatagt ccttccgaag gatgaagatg tgcccgggat tgaagaatcg
      781 aatcgcgtgc tgcagacgct gtcgtgcgcg cgaccgcgtg gcgcggaacc accgctgcca
      841 gaggcggatg agcgcccacc tgtgcggcac acggatgatc actttgccgc cgggcgcgac
      901 gagcttgtct aacgcagacc agagacggcg gatgtcgggc tcgaagtaca ggacgtcgct
      961 cagcacgacg atgtcgtagc gccgctcgtg ctcgtcgagc gcttgtgcac cgccggtgat
     1021 cgtcgggaat ccgaatcgct cggtcgcggc tttggtcccg gaggcggaga cgtcgttcac
     1081 gaccacatac agatccggac gccgttcgac gaggtgcgcc atgagatcgc cgcgcgcacc
     1141 gccgatgtcg agcacgtggg cgccgcgcgg ggcgagatcg tcgatgacgc gcgccaagtg
     1201 cccgaagatg cggcgtctcg agaag
//
LOCUS       DASUCJ010000012         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_443325, whole genome shotgun sequence.
ACCESSION   DASUCJ010000012 DASUCJ010000000
VERSION     DASUCJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443325"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>1273
                     /locus_tag="VFU90_00125"
     CDS             247..>1273
                     /locus_tag="VFU90_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5478199.1"
                     /translation="MSPSWPRPHSLSLSLSLAVFLGIATTECHRPTLSATEQHGQEIY
                     ARTCAVCHGPNGEGYAADQAPALAHPDFLASVSDDFLRSVITSGRSGTTMSAWGTAHG
                     GPLSPSDVDAVIARLHAWSRAPRAVLDERPVAGDISSGIGFYSTHCSSCHGTNGMNGT
                     NIHIGNHDLLASASNGFLRYAIVRGRPGTAMPAFAGVIGDEGIENVVALLRSWATAPA
                     PIQRPPPVSPLPLGPVPLNPHGPVPDGFARTPKTTPADTVKAQLDRGARMAILDARAP
                     QDYVNEHIAGAVSVPFYDPDRYFPDLPKDAWLVCYCSCPHAESGQLATKLLNAGFTKV
                     TVLDEGLG"
BASE COUNT          190 a          445 c          416 g          222 t
ORIGIN      
        1 cccgatccgc ctcgccgacg taatggatcg cagcgcgcgc caagcgatgg ttcagcggcg
       61 acggatagcg gcacggcccg aggatcttga tgtcggcgtc gccggggacg acgcgaccag
      121 ttcgttcggg gaccgttagg ccgggcgccg ccatctgcat ctgtacggta cggatcggac
      181 gtttcgcgct gctttcggaa tcgttgcacg ggccggcttg gagtaaggtt cgcctcgctc
      241 ccgcccatgt cgccttcttg gcctcgtccg cattcactct cgctttcgct gtcgctcgcg
      301 gtctttctgg gcatcgcgac gacggaatgc catcggccga cgctgagcgc gacggaacag
      361 cacggccagg agatctatgc gcgcacgtgc gccgtctgcc acgggccgaa cggggagggg
      421 tacgccgcgg accaggcgcc ggcgctggca caccccgatt ttctcgcgtc cgtgagcgac
      481 gacttcttgc gcagcgtaat tacgtccggc cggtcgggca ccacgatgtc cgcctggggt
      541 acggcgcacg gcggcccgct gtcgccgagc gacgtcgacg ccgtcatcgc ccgccttcac
      601 gcctggagcc gggcgccccg cgccgtcctc gacgagcgcc ccgtcgcggg ggacatctcg
      661 tcgggaatcg ggttttactc gacccattgc tcctcgtgcc acggcacgaa tgggatgaac
      721 gggaccaata ttcatattgg caatcacgat ctgctggcgt cggcgagcaa cggctttcta
      781 cggtacgcga tcgtacgcgg tcgtccgggg acggcaatgc ccgccttcgc gggcgtcatc
      841 ggagacgagg gtatcgagaa cgtcgtggcc ctcctgcgca gctgggccac cgctcctgcg
      901 ccgattcagc gcccgccgcc ggtgtcgccg cttccgctcg gacccgtgcc gctcaacccg
      961 catggcccgg tgcccgatgg ttttgcgcgt acgcccaaga cgacgcccgc cgatacggtg
     1021 aaagcgcagc tcgatcgcgg cgcacggatg gccattctcg acgcgcgcgc gccgcaggat
     1081 tacgtcaacg aacatatcgc gggagcggtg agcgttccgt tctacgatcc ggatcgctac
     1141 ttcccggacc tgcccaaaga cgcgtggctc gtctgctact gctcgtgtcc ccacgccgaa
     1201 tcggggcagc tcgcgacgaa gctcctcaac gccggcttca cgaaagtcac cgtcctcgac
     1261 gaggggctcg gcg
//
LOCUS       DASUCJ010000013         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1452002, whole genome shotgun sequence.
ACCESSION   DASUCJ010000013 DASUCJ010000000
VERSION     DASUCJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452002"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFU90_00130"
     CDS             complement(<1..698)
                     /locus_tag="VFU90_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478200.1"
                     /translation="MTAGATVLGFPRIGRDRTLKKATEAYWRGDIPAAALLQTAATIR
                     RENWLALRDAGLDEAPCNDFSLYDHMLDTAVLFGALPARHRHRVDPRLPDDEMSDDVR
                     LARYFAAARGLDGGAPLEMTKWFDTNYHYLVPELEPSARLSLNARKPMTELSEARALG
                     MSARPVVVGPITFLALSKPTENAPAGWTPWELLDELIPLYAGLLAQLRSAGAEWVQLD
                     EPVLATDLPSSVLE"
     gene            complement(695..1138)
                     /locus_tag="VFU90_00135"
     CDS             complement(695..1138)
                     /locus_tag="VFU90_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478201.1"
                     /translation="MKVTEMRRKRSSKRLERDAYRQVAIQVGQASAGGRAAEERMNEL
                     VRAGAPLSDVDAAYRAIGELAQEPLPAQPALDRNRPAELTRELSALRTRRQWYLMDAP
                     PGALPPAVVRPTSRAPKGPHVAGLDADFDERPWGVDLAQALDRLQ"
     gene            1270..1575
                     /locus_tag="VFU90_00140"
     CDS             1270..1575
                     /locus_tag="VFU90_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU5478202.1"
                     /translation="MSEWRNRAACRDVDPELFFPIGHGPAAEAQIVQAKAVCARCPVV
                     RECLAWALETGQDAGVWGGLTEEQRRQMRRDLLVLDADDLERRAEHAAKTGAGRAGD"
     gene            complement(1504..>1746)
                     /locus_tag="VFU90_00145"
     CDS             complement(1504..>1746)
                     /locus_tag="VFU90_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478203.1"
                     /translation="LAPWRVRHVLSAGGDAVTHGVDITDHFDKGVASLRAHAAYLAAL
                     GENPLADPEEFLESIARATGARLGCVFGAAFEVISV"
BASE COUNT          284 a          588 c          591 g          283 t
ORIGIN      
        1 aactcaagta cggacgacgg aaggtcggtc gcgagcaccg gctcgtcgag ttgcacccac
       61 tccgcccccg cggatcgaag ctgcgccagt aggccggcgt acaacgggat caactcgtcg
      121 agcaactccc atggagtcca gccggctggc gcgttctcag tgggtttgct taacgcgagg
      181 aacgtgatcg ggccgacgac aaccgggcgc gcggacattc ccagcgcgcg cgcctccgac
      241 aactcggtca tcggctttcg cgcgttcaac gacagccgcg cgctgggctc cagctctgga
      301 accagatagt ggtagttcgt gtcgaaccac ttcgtcatct cgagcggcgc cccaccatcg
      361 aggccacgcg cggcggcgaa gtagcgcgcc aaccgcacgt cgtccgacat ctcgtcgtcg
      421 ggcagtcgcg gatcgacccg gtgccggtgt cgagcgggta gcgcgccgaa cagcacggcg
      481 gtgtcgagca tgtggtcgta caacgagaag tcgttgcatg gcgcctcgtc gaggccagcg
      541 tcgcgaagcg cgagccagtt ctcccggcga atcgtcgccg ccgtctgcag gagcgctgcc
      601 gcagggatgt cgccccgcca gtaggcctcc gttgccttct tcagcgtgcg gtcacgtccg
      661 atccgcggaa acccgagaac ggtcgcgccc gccgtcattg caggcgatct aaggcttggg
      721 ccaggtcgac gccccacggc cgctcgtcga agtccgcgtc aagtccggcg acgtgcgggc
      781 cctttggcgc ccgcgacgtc ggccggacga cggcaggcgg tagcgcgcct gggggcgcgt
      841 ccatcaggta ccactgccgt cgcgttcgaa gcgccgagag ctccctggtg agttcggcgg
      901 gccggttgcg atcgagcgcg ggctgcgccg gcaacggctc ttgcgcgagt tcgccgatcg
      961 cgcgatacgc cgcatcgacg tcgctgagcg gcgcgcctgc gcgcaccagc tcgttcattc
     1021 gctcctccgc cgcccggccg cccgcactcg cttgccccac ctggatcgcc acctgccggt
     1081 aggcatctct ttccagccgc ttacttgacc gcttgcgccg catctccgtc accttcattc
     1141 tcacaatccc gatcgtagaa aagatcggcc ggagccgcta tggcctggat caccaaggaa
     1201 ggtaaacact agcctcattg tgtaaacagc gttacgttcg agcggggtcg aaaagagttc
     1261 ggagccgtga tgagtgagtg gcgtaacaga gcggcgtgcc gcgacgtcga ccccgagttg
     1321 ttcttcccga tcggccacgg cccggccgcg gaggcgcaga tcgtccaggc taaagcggtc
     1381 tgtgcgcgtt gcccagtggt ccgggaatgc cttgcgtggg cgctcgaaac cggccaagac
     1441 gccggggttt ggggcgggct gacggaggag caacggcgcc agatgcgtcg cgatctgctt
     1501 gtgctagacg ctgatgacct cgaacgccgc gccgaacacg cagccaagac gggcgccggt
     1561 cgcgcgggcg attgactcca agaactcttc tgggtcggcg agcggattct cgccgagcgc
     1621 ggcgaggtag gcggcgtgcg cgcgcaacga ggcgacacct ttgtcgaagt ggtcggtgat
     1681 gtcgacgccg tgggtgacgg cgtcgccgcc ggccgacagc acgtgtcgca cccgccacgg
     1741 tgccaa
//
LOCUS       DASUCJ010000014         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_917099, whole genome shotgun sequence.
ACCESSION   DASUCJ010000014 DASUCJ010000000
VERSION     DASUCJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917099"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFU90_00150"
     CDS             <1..543
                     /locus_tag="VFU90_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5478204.1"
                     /translation="RAAGTFTYPARFQLVASMNPCPCGYRGTRSAECRCDDAAVAKYV
                     GKLSGPLLDRIDLQIEIGRVPFDDMVRGEAAERSPQICRRVLAARERQQARFSGSGLR
                     CNAEIPGNAMRRYCALDEPAMRLLARASAKRQFSARALDRIARVARTIADLAGAGAIA
                     AEHVAEAVQYRSLERIGAAV"
     gene            591..917
                     /locus_tag="VFU90_00155"
     CDS             591..917
                     /locus_tag="VFU90_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5478205.1"
                     /translation="MASITIDDTARFVSVLSFSLGPGDVSGMTREVRHAVEGRVPNEP
                     GFIGCVVMVNAETAQLLVVSVWESRHAWSAAQYDREIGQVVSDVVETAKSYQIQTYET
                     LTVVRA"
     gene            complement(928..>1817)
                     /locus_tag="VFU90_00160"
     CDS             complement(928..>1817)
                     /locus_tag="VFU90_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478206.1"
                     /translation="KTQLTYTSGSNETGGLPLPKFIVDEIELLSAGSIGRNTSYYLEQ
                     YAVDGGVPGESRDVWVNFDRYGRAGDPIGPALHAKLGQFTLPLPVDPETQRPTLSSYL
                     LYDQTVGDNPFDLFDPGLGADLYFTDERHGFETHVDVLEAYTRRSGLPASGVDFMATV
                     SKTLGDDFTVYAYRYQGRQQLTPVTDSFYRQAYGVGYESGKFAAVGLVQNGYDTSPLG
                     NGAGAVSSGGFLQASWTFDDALALYARYDGVYDPFTQTTRQGTLSLVMRPASRYRLTL
                     EGTKSEGSYQINAGLLFAY"
BASE COUNT          325 a          567 c          620 g          305 t
ORIGIN      
        1 cgcgcggcgg ggacgtttac gtatccggcc cgctttcagc tcgtcgcatc gatgaatccg
       61 tgcccgtgcg gctaccgcgg gacgcgcagc gccgagtgcc gctgcgacga cgcggccgtc
      121 gccaaatacg tcggcaagct ctcgggaccg ctgctcgacc gcatcgactt gcagatcgaa
      181 atcgggcgcg tgccgttcga cgacatggtg cgcggcgaag ccgccgagag gtcgccgcag
      241 atttgccggc gcgtgctcgc ggcgcgcgaa cgccagcagg cacggttttc cggcagcggc
      301 ctgcgctgca atgccgagat tccgggcaac gcgatgcgcc ggtactgcgc gctcgacgaa
      361 ccggcaatgc ggctgctcgc acgcgcgagc gccaaacggc agttttcggc gcgcgcgctc
      421 gaccggatcg ctcgggtcgc acgcacgatt gcggatctgg ccggcgcggg cgcgatcgcc
      481 gcggagcacg tcgccgaagc cgttcaatac cgcagcctcg aacggatcgg cgcggccgtc
      541 taacgtgcgt acgaggagga agtcggaacg tcgtggacgg accggccggg atggcctcaa
      601 ttacgatcga cgacacggca aggttcgtca gcgttttatc gttcagctta ggtccgggcg
      661 acgtgtccgg catgacgcgc gaggtacgcc acgccgtcga aggccgagtc ccaaacgagc
      721 cgggcttcat cggatgcgtc gtgatggtga acgccgaaac agcgcagctg ttggtggttt
      781 cggtatggga atctcgacac gcgtggagcg cggcgcagta cgatcgggag atcgggcaag
      841 tggtcagcga cgtcgtggaa accgcaaagt cgtaccagat tcagacgtac gaaacgctga
      901 cggtcgtgcg agcctagcga tggccgccta atacgcaaac agcaggccgg cgttgatctg
      961 atagctccct tccgatttcg taccttcgag cgtcaaccgg taacgggacg cgggccgcat
     1021 caccagcgat agggtgccct gtcgcgtcgt ttgagtgaac gggtcgtaaa cgccgtcgta
     1081 acgcgcgtag agcgcaagcg catcgtcgaa cgtccacgac gcttgcagaa agccgcccga
     1141 cgaaacggcc ccggccccgt taccgagcgg gctcgtgtcg tagccgttct gaacgagccc
     1201 gacggccgcg aacttcccgc tttcgtaccc cacgccgtaa gcttggcgat agaacgagtc
     1261 ggttaccgga gtcaactgct ggcgcccctg atagcgatag gcgtagacgg tgaaatcgtc
     1321 tccgagcgtt ttcgagaccg tcgccatgaa gtcgacgccg gacgcgggga gcccgctgcg
     1381 gcgcgtgtag gcttcgagca cgtcgacgtg cgtttcgaag ccgtgccgtt cgtcggtaaa
     1441 gtacagatcg gcgccgagcc ccggatcgaa caagtcgaac ggattgtcgc cgaccgtttg
     1501 atcgtagagc agataactcg aaagggttgg gcgctgcgtc tcgggatcga ccggcagcgg
     1561 cagcgtgaac tgtcccagct tggcgtgcag cgcgggcccg atcggatcgc cggcgcggcc
     1621 gtaccggtcg aagttcaccc acacgtcgcg cgactcgccc gggacgccgc cgtcgaccgc
     1681 gtactgctcg aggtagtacg acgtgtttcg gccgatcgat ccggccgaca gcagctcgat
     1741 ttcgtcgacg atgaatttgg gcagcggcaa gccgccggtt tcgttggagc cgctcgtgta
     1801 ggtgagctgc gtcttga
//
LOCUS       DASUCJ010000015         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_137631, whole genome shotgun sequence.
ACCESSION   DASUCJ010000015 DASUCJ010000000
VERSION     DASUCJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137631"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1782
                     /locus_tag="VFU90_00165"
     CDS             232..1782
                     /locus_tag="VFU90_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478207.1"
                     /translation="MFRSWNKVAALALAFGFGAAGCSGSHSLMPAGPAASGNNLRARI
                     AQGGRIPLRGAVPLASQPPEGLQMAFLMTDGTVLAQSYAGNTWYRYAPDASGNYSNGT
                     WTQAATLPTGYAPSAFSSAVLADGRLLISGGEYNTPGNYDLQLVNLGAVYDPVKNTWK
                     SLGHPVGWKWIGDSPASILPDGRFLIGQKLTKQVAALTPSTLHFTKLTYKGKADFNAE
                     EGWTLLADGTILTADVKKAPHAEIYNAATGTWKSAGSTIVDLHSPSPYHQCLPYGPAP
                     QDCYLPPGEIGPLMLRPDGTVFATGSYSSGYGVGHTSIFHSLGSQAGTWTVGPDFPNG
                     DNAGDSFSVLLPSGNVLVFGVSGTLYEFDGTKFAQTGSGCCAPLLLPTGQVMIINYST
                     VSLYTPSGQPQSSWLPTISSVQKTLTRGQTYTITGTQFNGLSQAMSFGDEFQNATNYP
                     LVRITNTSSGHVFYARTHDHSSMGVATGTASVNTHFDVPSTMETGASTLQVVANGIAS
                     KTVNVTVK"
     gene            1926..>2146
                     /locus_tag="VFU90_00170"
     CDS             1926..>2146
                     /locus_tag="VFU90_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478208.1"
                     /translation="MSTYRAAASFAALLALAACSAGGRWAAVPPTAASELRGAAGSGA
                     GKIDHVVFIIQENRSFDNLFQGYPGADTVS"
BASE COUNT          377 a          718 c          664 g          387 t
ORIGIN      
        1 gggcccaagc cgctggcgaa tctcgccgcg ctgcgcgcac tcattcgcgg cgcgtcggcg
       61 ggcgggtgga cgcagcaatc gatcaagggc gacttttaga gcggccaagc gacgtccccc
      121 gacgccgtcc acaccacgcg ccggtaaaga gcggcgcaga gcgggaaatc gtccccggcc
      181 ccccaaagga gcctagcctt cggcgctctt tttttttgtg gaggtcctcc cgtgtttcgc
      241 tcttggaaca aagtagctgc tttggcgttg gcgttcgggt tcggcgccgc cggctgctcc
      301 ggcagccatt cactgatgcc ggccggcccg gccgcttccg gcaacaacct tcgcgctcgt
      361 atcgcgcaag gcggccgcat accgctgcgc ggtgcggtgc cgctggccag tcagccgccg
      421 gaaggactcc agatggcgtt cctgatgacg gacggtacgg tcctcgcgca gagttacgcg
      481 ggcaatacgt ggtaccgtta cgcaccggat gcttcgggca actattccaa cggaacgtgg
      541 acgcaagcgg cgacgctccc aaccggctac gcaccgtcgg ccttttcctc ggccgtgctc
      601 gccgacggcc ggctgctgat cagcggcggc gaatacaaca ctcccggcaa ctacgacttg
      661 cagctcgtca atctgggcgc cgtatacgat ccggtcaaga acacgtggaa gtcgctcggc
      721 catcccgtcg gatggaagtg gatcggcgac tcgccggcca gcattctgcc ggacggccgt
      781 ttcctgatcg gacaaaaact caccaaacaa gtcgccgcgc tcaccccctc gacgctgcac
      841 tttacgaagc tgacctataa ggggaaggcc gacttcaatg ccgaggaggg ctggacgctg
      901 ctcgccgacg gcacgatttt aaccgccgac gtcaagaagg cgccgcatgc ggaaatctac
      961 aacgcggcga ccggtacgtg gaagtcggcc ggctcgacga tcgtggactt gcattcgccc
     1021 tcaccgtacc atcaatgcct accctacggg ccggcgccgc aggactgcta cctgccgccc
     1081 ggcgagattg ggccgctgat gttgcgtccc gacggcaccg tcttcgccac cggttcgtac
     1141 tccagcggct acggcgtcgg tcacacctcc atcttccata gcctcggaag ccaggcggga
     1201 acgtggaccg tcggtccgga tttccccaac ggcgacaacg ccggcgattc gttctccgtg
     1261 ttgcttccca gcggaaacgt gctggttttc ggggtgagcg gcaccttgta cgagttcgac
     1321 ggcacgaagt tcgcgcagac cggctcgggc tgctgcgcgc cgcttctgct gccgaccgga
     1381 caagtcatga tcatcaacta ctcgacggtg agcctgtaca cgccgtcggg tcaaccgcag
     1441 tcctcgtggc tgccgacgat ttcgtcggtg cagaagacgc tgacgcgcgg ccaaacctat
     1501 acgatcacgg gaacgcagtt caacggcctc tcgcaagcga tgtcgttcgg cgacgagttt
     1561 cagaacgcga ccaactaccc gctggttcgc atcacgaaca cgtcgagcgg tcacgtgttc
     1621 tacgcgagga cccacgatca cagctcgatg ggcgtcgcaa ctggaacggc cagcgtgaac
     1681 acgcacttcg acgttccgtc gacgatggag accggcgcct cgaccctgca agtcgttgcc
     1741 aacggcattg cgtcgaagac ggttaacgtc acggtgaagt agctccggct gcttccagac
     1801 ccgccgtgcc gccgccggac gctaatccgg cggcggcttt ttcttgcagg cagtacgcgg
     1861 cggcgcgtgc ggtgaactcg gctaggcggc atcgagtgcc gcttcgatac ggaggattcc
     1921 tcgaggtgtc tacctatcgc gccgccgcct cgtttgcggc gttgctcgcg ttggcggctt
     1981 gctctgccgg cggacgctgg gcagccgtcc ctccgaccgc cgcgagcgag ctcagaggcg
     2041 ccgccggaag cggcgccggc aagatcgatc acgtcgtctt catcattcaa gagaatcgca
     2101 gtttcgacaa tttattccag ggctatccgg gcgccgatac cgtttc
//
LOCUS       DASUCJ010000016         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1436767, whole genome shotgun sequence.
ACCESSION   DASUCJ010000016 DASUCJ010000000
VERSION     DASUCJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436767"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="scpB"
                     /locus_tag="VFU90_00175"
     CDS             <1..586
                     /gene="scpB"
                     /locus_tag="VFU90_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5478209.1"
                     /translation="AARVESEADAAALEEAVLRLQRPVEALLFVAGEPLSIKQIARLV
                     RASEVEVAATLAQIGADFTDRGIVLREVAGGYRFATSPLARDAVEAYLLPPKTSLSSP
                     ALEALAIVAPLQPVTKSEVEAIRGVNSDSVVTTLLDRNLIEEAGRKDVVGRPMQYRTT
                     PFFLESFGLRSLDDLPELELEPGQPLELNLLAPS"
     gene            583..1437
                     /locus_tag="VFU90_00180"
     CDS             583..1437
                     /locus_tag="VFU90_00180"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEU5478210.1"
                     /translation="MNVGLHVRIGRGYAKAVEHAKAAGCTAMQVFSSNPKSYRPAAID
                     VPALEAFAALRRRAGLDPCAIHTPYLINLASDDAKIYQGSLRLLQHDLAVAARGGMRF
                     VNTHLGSYGTRDRKDGFAAVCRALETTLSEIDPGVFLVLENSAGAGNLAGGTVEELGS
                     FVKAIGHPQLGVCLDTAHAWAAGYEINTKAGVDRFIGLAGERIGIDRLLMFHFNDTEV
                     ALGAARDRHWHIGEGRIGFDGFRALLAHPELRGKTAILETPGDDADDLRNVQTILAIQ
                     RGALSGKA"
     gene            complement(1444..1581)
                     /locus_tag="VFU90_00185"
     CDS             complement(1444..1581)
                     /locus_tag="VFU90_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478211.1"
                     /translation="MFHDPIRSTPLVSDISDVTLRLKVRRADSALFAASDMRLPHLRV
                     S"
     gene            1677..2234
                     /locus_tag="VFU90_00190"
     CDS             1677..2234
                     /locus_tag="VFU90_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU5478212.1"
                     /translation="MASPSESDQAPARLDVLRRFVNTLDYPAGPDALGTPGEAAKWCR
                     AAGLPPVTNQAETERLRGFREALREVLFANNGESTQAAAWEGMRPYLTAVAFQPVADG
                     RGGIELEPAGAADGAIGTMLVIAYEAMAAGTWSRLRACRKSSCRFAYYDCSRNGSRAW
                     CSMAVCGNREKAQRRRTRERHGGLA"
     gene            2236..>2771
                     /locus_tag="VFU90_00195"
     CDS             2236..>2771
                     /locus_tag="VFU90_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5478213.1"
                     /translation="MTRFVAHFDVDAFYASVAVRDDPSLRGKPVAIAGRSRRAVVLTA
                     SYEARPFGVRSAMPLYRALAACPELVVVPPDHTKYRTVSREIFGVFGACGRAVEGLSL
                     DEAFVDAGETPFEQARSFAETIRRDVLAATGLTVSAGVAAGKMIAKIASDTCKPDGLL
                     AIAPGEERTFLAPLPVGRL"
BASE COUNT          459 a          894 c          947 g          471 t
ORIGIN      
        1 ggcggcgcgc gtcgagagcg aagccgacgc cgccgccctc gaggaagcgg tcttgcggct
       61 gcagcgtccg gtcgaggcgc tgttattcgt tgcgggcgaa ccgctctcga tcaaacagat
      121 cgcacggctg gtgcgcgcct ccgaggtcga ggtggctgcc acgctggccc agatcggcgc
      181 cgactttacc gatcgcggga tcgttttgcg cgaggtcgcc ggcggttacc ggtttgcgac
      241 gtcgccgctc gcgcgcgacg cggtcgaggc gtacctgctt cctccgaaga cgagcttgtc
      301 gtcgccggcg ctggaagcgc tggcgatcgt cgcccccctg cagccggtca ccaagagcga
      361 agtcgaagcg atccgcgggg tgaactccga cagcgtcgtc acaacgctgc tcgaccgaaa
      421 cctcatcgaa gaagccggcc gcaaagacgt ggtcgggcgg ccgatgcaat accgtacgac
      481 cccatttttc ttggaatcct tcggactgcg gtcgctcgac gatttacccg aactcgagct
      541 cgagccgggt cagccgctcg aactcaattt gctcgctccg tcgtgaacgt cggcctccac
      601 gtccggatcg ggcgcggata cgcgaaggcg gtcgaacacg cgaaggcggc cggctgtacc
      661 gccatgcaag tattctcttc gaatccgaaa agctatcgcc cggctgcgat cgacgttccg
      721 gcactcgaag cgtttgcggc cctgcggcgc cgggccggtc tcgatccctg cgcgattcac
      781 acgccgtatt tgatcaatct cgccagcgac gatgccaaaa tctatcaagg ctcgctgcgg
      841 ctgctgcagc acgatttggc ggtggccgct cgcggcggaa tgcgtttcgt caacacgcac
      901 ctcggctcgt acggaacgcg cgatcgaaag gatggattcg cggcggtctg ccgcgcgctc
      961 gaaacgacgc tgagcgagat cgatccgggc gtttttctgg tacttgaaaa ctcggccggt
     1021 gccggcaact tggccggcgg gacggtcgaa gagctgggga gtttcgtgaa agcgatcggg
     1081 catccgcagc tgggcgtttg cctcgatacg gctcacgcgt gggcggccgg ctacgaaatc
     1141 aatacgaagg cgggcgtcga tcgcttcatc gggctagccg gcgagcgcat cggcatcgat
     1201 cgcctgctga tgttccactt caacgatacg gaagtggcgc tgggcgccgc gcgcgaccgg
     1261 cactggcaca tcggcgaagg ccggatcggc ttcgacggat tccgcgcgct gctcgcgcat
     1321 ccggagctgc gcggcaaaac ggcgattctc gaaacgcccg gcgacgacgc cgacgatctt
     1381 cgaaacgtgc aaacgattct ggctattcaa cgcggcgcgc tctcggggaa agcttgacgc
     1441 ttcttacgag actcgcagat gcggaaggcg catgtccgaa gcggcaaaca gcgccgagtc
     1501 ggcgcgccgg accttgagcc ggagcgtcac gtccgagatg tcggacacga gcggcgtgga
     1561 gcggatgggg tcgtgaaaca tggtgatcct cgtaaagtgc agctggtaac ctactaacat
     1621 gatacgatgg ttaaagtaac ttgtcaagtc gttttggtgg tgctaagctc gcagtaatgg
     1681 cgtccccttc cgaatccgat caggctcccg ctcggctcga cgtgctgcgc cggttcgtca
     1741 acaccctcga ctatccggcg ggcccggatg cactcggcac cccgggcgag gcggcaaaat
     1801 ggtgccgcgc ggcgggcctt cctccggtaa cgaaccaggc ggagacggag cggttgcgcg
     1861 gctttcggga ggcgctgcgc gaggtgctgt ttgcgaacaa cggcgagtcg acgcaagccg
     1921 ccgcgtggga ggggatgcgg ccgtacttga cggccgtcgc cttccagccg gtcgccgacg
     1981 gccgcggcgg gatcgagctg gagcccgccg gcgccgccga tggggcgatc ggaacgatgc
     2041 tcgtcatcgc gtacgaggcg atggcggccg ggacgtggtc gcggctgcgt gcctgccgga
     2101 agagcagctg ccggtttgct tattacgact gctcccgtaa cgggtcgcgc gcgtggtgca
     2161 gcatggcggt ctgcggcaac cgggagaaag cgcagcgccg gcgcacgcgc gagcggcacg
     2221 gcggactcgc ctagcgtgac gcgattcgtc gcgcacttcg acgtcgacgc gttctacgcg
     2281 agcgtcgccg tccgcgacga tccgagttta cgcggcaagc cggtcgccat cgccgggaga
     2341 agccggcgcg cggtcgtgct gacggcgtcg tacgaagcgc ggccgttcgg cgtccgttcc
     2401 gcgatgccgc tgtaccgcgc cctcgccgcg tgccccgaac tcgtcgtcgt tccgcccgac
     2461 cacaccaaat atcgcaccgt ctcgcgcgag atcttcggcg ttttcggcgc gtgcggccgc
     2521 gccgtggagg gcctctcgct ggacgaggcc ttcgtcgacg ccggcgagac gccgttcgaa
     2581 caagcgcgtt cgttcgccga gacgattcgg cgcgacgtgc ttgccgcgac cggtctcacc
     2641 gtgagcgcgg gcgtcgccgc cggaaagatg atcgcaaaaa tcgcatccga tacgtgtaaa
     2701 cccgacggct tgctcgcgat cgcgccgggg gaagagcgca ccttcctcgc accgcttccg
     2761 gtcggccggc t
//
LOCUS       DASUCJ010000017         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_504505, whole genome shotgun sequence.
ACCESSION   DASUCJ010000017 DASUCJ010000000
VERSION     DASUCJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504505"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFU90_00200"
     CDS             <1..905
                     /locus_tag="VFU90_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478214.1"
                     /translation="EQLIQMDYAICILDLEGDHLAIGFLHGAVALGGREPLPPPAQVD
                     RVIRQGFSSVVIDLSLQTPDVKRRYALAILEQLRLTRSTVGLPHWIIVDEAHVPIAGG
                     LRHWWRDSDESGICVITYRPDLLAPELEQYADFVITARADHLASLRSRGGDGPGRAFT
                     AAARLTAHVRHWHKYIEGQLPTERRFYFRDRHGLTGRSAGNLLEFHDGVRHASAETLR
                     HHGSHGDFSRWLASVCPAPSIVKAALVTEADLARATTDTDVERLRDQLLRALDGKYST
                     PPSDARPESGDGSAAETTAHAEAQ"
     gene            998..2659
                     /locus_tag="VFU90_00205"
     CDS             998..2659
                     /locus_tag="VFU90_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5478215.1"
                     /translation="MAGHTARAQAADTLDVPTALREAIISSMARAANGTPQLDEALVR
                     LYARRQWSPAWLADGRPTPQAAAVVAFLGRVPALGLPPEAYDAQGLADEMALMAATPL
                     GLTERIRFDVSLSRSLLLLVSHLAQGRVDPASLGFSLSRGPDIDLAAAARDVSTADDV
                     PAAIRRVEPQYAGYKALEAALARYQALASDSTLRPPHVTRASLRPGDTYADMAALRRL
                     LFAFGDLATDSTTRADTANPLAYTSEIVAAVERFQTRHGLEADGIVGPATFAALRVPI
                     THRVTQMELSLERWRWLPRRPPPRYIAINIPAFRLFAFDSDSIAAHPDATMKVILGAS
                     RMRRATPVFEGTMRQIVFRPYWDVPKSIAIKELIPLLRPDTGYALHQSLEIVRGPDVH
                     ATLFPITARNLDRVAAGTLRLRQRPGANNALGLVKFVFPNAHNVYLHGTPETELFLRA
                     RRDFSHGCIRVEDPVGLAAFALGGQDDWSPSSIMAAMHADQPRTVTLARPIEVFILYA
                     TAVVNRDGAIDFYPDLYGHDAALARVLDQATARAAQPRRPLAEDL"
     gene            2689..3999
                     /locus_tag="VFU90_00210"
     CDS             2689..3999
                     /locus_tag="VFU90_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEU5478216.1"
                     /translation="MIRDAARPLTGAHEDFDPVMALIGDARVVLIGEASHGTHEFYRI
                     RAEITKRLIRERGFVAVAAEADWPDAYRVNRYVRGAGTDADATDALDGFRRFPQWMWR
                     NADVLDFVGWLREFNDPVPNDARKVGFYGLDLYSLHASMAAVLDYLRIVDPAAAKRAQ
                     YRYACFEQFGEDPQAYGYAASYGLAPSCEREVVEQLVDLRRSAADYAQRDGRLAPDDL
                     FFTEQNARVVRNAERYYRAMFGSRVSSWNLRDQHMADTLQALVSFLDRSGSRSKIVVW
                     AHNSHVGDARATELGAGGELNVGQLAREQFGPDVVALGFTTHHGTVTAASDWDTPAQR
                     KVVRPSLPESYEALFHEGPRNFFLDLRTPSAVAPALDDVRIERAIGVIYRPETERLSH
                     YFSARLRRQFDGVLHYDETRAVEPLERTGVWDRGEVPETFPTAL"
BASE COUNT          653 a         1395 c         1305 g          675 t
ORIGIN      
        1 ccgagcagct gatccagatg gactacgcga tctgcatcct cgatctcgaa ggcgaccatc
       61 tcgcgatcgg cttcctgcac ggcgcggttg cgctcggcgg gcgcgagccg ctgcctccgc
      121 ccgcgcaggt cgaccgggtg atccggcaag ggttcagctc ggtggtgatc gacctctcgt
      181 tgcagacgcc cgatgtgaag cggcgttatg cgctcgcgat cctcgagcag ctgcgcctaa
      241 cgcggtccac ggttggtctt ccgcattgga tcatcgtcga cgaagcacac gtgccgattg
      301 cgggcgggct ccgccattgg tggcgcgact cggacgagag cggcatctgc gtcatcacgt
      361 atcggccgga cttgctggcg cccgagctgg agcaatacgc cgacttcgtg atcaccgcgc
      421 gcgcggacca tctggcgagc ctgcgatccc gcggcggcga cggccccgga cgcgccttca
      481 cagccgccgc gcggctcacc gcgcacgtga ggcactggca caagtacatc gaaggccagc
      541 tgcccaccga gcggcggttt tatttccggg accgtcacgg cctaacagga cgcagtgcgg
      601 gcaacctgct cgagttccac gacggggtcc gccacgcgtc ggccgagacg cttcgtcacc
      661 acggctcgca cggcgatttt tcgcgatggc tggcgagcgt gtgccctgcg ccgtcgatcg
      721 tgaaggcggc gctggtcacg gaggccgatc tggcacgggc cacgacggac accgatgtgg
      781 agcgcctgcg cgatcagcta ctccgcgcac tcgacggcaa atacagcacg ccgccatccg
      841 atgcgcggcc ggaatccggt gacggatccg ccgccgaaac gacggcccac gcggaagcgc
      901 agtagccgcc atgcaaacgc gaaggcgcct atcgttcgcc ggccacctcc ggcgggtgct
      961 cctctgttgg gcgtgctggg tcggcgttgg gcttgtcgtg gcgggacaca cggcccgcgc
     1021 acaggcggcc gacacgctcg atgtcccaac cgcgttgcgg gaagcgatca tctcgagcat
     1081 ggcccgcgcg gcgaacggaa caccgcaact ggacgaagcg ttggtccggt tgtacgcccg
     1141 gcgccagtgg tcgccggcgt ggctcgcgga cggacgcccg acgccgcagg cagccgccgt
     1201 agtcgctttc ctgggccgcg tgccagcgtt aggcctgccg ccggaagcct acgacgccca
     1261 gggtctcgcc gacgagatgg cgctgatggc ggcaaccccg ctcggcctaa ctgaacgcat
     1321 tcgattcgac gtgtcgctgt cccggtcgct cctgttgctc gtgagccatc tggcccaggg
     1381 acgcgtcgac ccggcgtcgc tcggcttctc gctttcgcgt ggccccgaca tcgacctcgc
     1441 ggccgcggcg cgcgacgtca gcaccgccga cgatgtgccc gcggcaattc gacgagtcga
     1501 gccgcaatat gccggctaca aagcgctcga ggctgcgctc gcccgatatc aagcgctcgc
     1561 ctcggactcc acgcttcgac cgccgcacgt cacgcgtgca tcccttcggc caggcgacac
     1621 ctacgccgac atggccgcac tgcgccggct gctcttcgcc tttggcgatc tggcaaccga
     1681 ttccacgacg cgcgccgata cagccaatcc gctggcatat acgtccgaga tcgtcgccgc
     1741 ggtcgaacgc tttcagaccc gacacggcct cgaggccgac ggcatcgtcg ggcctgccac
     1801 gttcgccgcg ctgcgcgtgc cgattaccca ccgcgtgacg cagatggaat tgagtctcga
     1861 acgctggcgc tggctcccgc gccggccgcc gccgcgctac atcgccatca acatcccggc
     1921 cttccgattg ttcgcgttcg acagcgactc catcgcggcg catcccgatg ccacgatgaa
     1981 agtgatcctc ggcgcatcgc ggatgcgccg ggcgacgccg gtgttcgagg gcacgatgcg
     2041 acagatcgtg tttcgcccgt actgggatgt gccgaagagc atcgcgatca aagagctcat
     2101 cccgcttctc cggccggaca ccggctacgc gctgcaccag tctctcgaaa tcgtgcgcgg
     2161 gcccgatgtg catgcgacgc tgtttcccat cacggctcga aatctcgacc gcgtcgccgc
     2221 cggcacgctg cgtttgcgcc agcgacccgg cgcaaacaac gcgcttggcc tcgtgaaatt
     2281 cgtcttcccg aatgcacaca acgtgtacct gcacggcacg cccgagacgg agctcttcct
     2341 tcgcgcacga cgggacttca gccacgggtg catccgggtc gaagaccctg tcggcctcgc
     2401 ggcgtttgcg ttaggcggcc aggatgactg gtcgccgtcg tcgatcatgg ccgcgatgca
     2461 cgccgaccag ccccgcacgg tgacgctcgc gcgccccatc gaggtgttca tcctctatgc
     2521 gaccgccgtc gtgaaccgcg acggcgcgat cgacttctat cctgacctgt acggacacga
     2581 cgcggccctc gcccgcgtgc tcgaccaggc gaccgcgcgc gccgctcaac ccagacgccc
     2641 cttggcggag gacctatgac cagccccgtt cggcgatcac tcgcccacgt gatacgcgat
     2701 gcggcgcgcc cgttgaccgg cgcgcacgag gacttcgatc cggtcatggc gctcatcggc
     2761 gatgcgcgcg tggtgctcat cggcgaagcc tcgcacggca cgcacgagtt ctaccgcatt
     2821 cgggccgaga tcacgaagcg gctcatccgt gaacgcggct tcgttgccgt ggcagccgag
     2881 gccgactggc cggatgcgta tcgcgtgaac cgctatgtgc gcggtgccgg caccgatgcg
     2941 gacgcgacgg acgcgctcga tggattccgg cgattcccgc aatggatgtg gcgcaacgca
     3001 gacgtcctcg atttcgtcgg ctggctgcgc gagttcaacg acccggtgcc taacgatgca
     3061 cgaaaagttg ggttctacgg tttggatctc tacagcctgc acgcgtcgat ggcggcggtg
     3121 ctcgactatc tgcgcatcgt cgacccggcg gcagcgaagc gggcgcaata ccggtacgcg
     3181 tgcttcgagc agttcggtga agatccgcag gcgtacggct acgcggcgag ctatggcctg
     3241 gctccctcct gcgaacggga ggtggtggag caactcgtcg accttcggcg ctcggctgcc
     3301 gactatgcgc agcgcgacgg acgactggcg ccggacgacc tgttcttcac cgagcagaat
     3361 gcccgagtgg tgagaaatgc cgaacgctac tatcgcgcga tgttcggcag ccgggtttcg
     3421 tcgtggaacc tgcgcgacca gcacatggcc gacacgctgc aggcgctggt ctcgtttctg
     3481 gaccgatccg gctctcgctc aaagatcgtg gtgtgggcgc acaattcgca cgtgggagat
     3541 gcacgcgcca ccgagctcgg cgcgggaggc gagctgaatg tcggccagtt ggcccgcgaa
     3601 cagtttggtc ccgacgtcgt cgcgctcgga ttcacgaccc accacggaac ggtaactgcc
     3661 gcctccgact gggatacgcc ggcgcaacgc aaggtcgtgc gaccgtccct gcccgagagc
     3721 tacgaggcgc tgtttcatga ggggccgcgt aatttcttcc tcgatctgcg tacgccgagc
     3781 gcggttgcac ccgcgctcga cgatgtgcgg atcgaacgcg cgatcggcgt gatctatcgc
     3841 cccgaaacag agcggttgag ccactatttc tccgcgcgat tacggaggca gttcgacgga
     3901 gttctccact acgatgagac acgggccgtc gagccgctcg aacggaccgg agtctgggat
     3961 cgcggtgagg tccccgagac gtttccgact gcactctaaa atcgtatcgc gcgacgcatc
     4021 agacggtc
//
LOCUS       DASUCJ010000018         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_565676, whole genome shotgun sequence.
ACCESSION   DASUCJ010000018 DASUCJ010000000
VERSION     DASUCJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565676"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFU90_00215"
     CDS             complement(<1..451)
                     /locus_tag="VFU90_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478217.1"
                     /translation="MNDFNTGDEVPFNRRASATAYSWAMNRRHALGAALVGGLAFASA
                     AKAASALDSSATVPVKSIPVPKNGEISVAFVVSDRANLIDLAGPWETFQDMMLPNGDM
                     PFHVYTVAETLAVIETVSGLRIEPTYSFDSLPGVPNVIVVGAQSNQSP"
     gene            486..>1007
                     /locus_tag="VFU90_00220"
     CDS             486..>1007
                     /locus_tag="VFU90_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478218.1"
                     /translation="MIEKLQPGQWYGRIERAQTIGGVLLSVVHHDRPKQIAVHEHALS
                     YFLMPFAGGYRETHDGKTIQYDPFSIAFHPAGFVHSDEVFGPEKFFAIELQPEWQSRV
                     GHLFDISAWHFELQHGEAVWLAVALLQRFLNDELEALEVDAVVSEMLGIALRLVDRDR
                     PQRAWVTDVKRVLR"
BASE COUNT          185 a          330 c          304 g          188 t
ORIGIN      
        1 tcggcgactg gttcgactgc gcgcccacga cgatgacgtt cggaaccccc ggcagcgagt
       61 cgaacgaata cgtcggctcg atgcgcaatc cgctcaccgt ttcgatcacg gccagcgttt
      121 cggccaccgt gtacacgtga aacggcatgt caccattggg cagcatcatg tcttggaagg
      181 tttcccacgg cccggcgaga tcgatgaggt tcgcgcgatc cgacaccacg aacgctaccg
      241 agatctcgcc gttcttcggc accgggatcg atttgacggg caccgttgcg gagctgtcca
      301 gggcggaggc cgcttttgcg gccgaggcga aggcgaggcc gcccaccagg gcggcgccga
      361 gagcgtgtcg tcgattcata gcccaactat aagccgtcgc gctcgcccgg cgattgaacg
      421 gaacctcatc gcccgtgtta aaatcgttca agacccgtcg ttccccggga accgatgatg
      481 acgccgtgat cgaaaaactc cagcccggcc aatggtacgg ccgaatcgaa cgcgcgcaga
      541 cgatcggcgg cgtgctgctg tcggtcgtgc atcacgaccg gccgaaacaa atcgccgttc
      601 acgagcatgc gctgtcgtac ttcttgatgc cgtttgccgg cggttatcgc gaaacgcacg
      661 acggcaaaac gattcagtac gatccgttca gcatcgcctt ccatccggcc ggcttcgtcc
      721 actccgacga agttttcggt cccgagaagt tcttcgcgat cgagctgcag cccgagtggc
      781 agtcgcgcgt cggccatttg ttcgatatct ccgcctggca cttcgagctg cagcacggcg
      841 aagcggtctg gctcgcggtc gcgctgctcc agcgcttcct caacgacgaa ctcgaagcgc
      901 tcgaagtcga tgcggtcgtc tccgaaatgc tcggcatcgc actgcgcctg gtcgatcgcg
      961 atcgcccgca gcgcgcgtgg gtcaccgacg tcaaacgcgt gttgcgc
//
LOCUS       DASUCJ010000019         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1513202, whole genome shotgun sequence.
ACCESSION   DASUCJ010000019 DASUCJ010000000
VERSION     DASUCJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513202"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1355)
                     /locus_tag="VFU90_00225"
     CDS             complement(<1..>1355)
                     /locus_tag="VFU90_00225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5478219.1"
                     /translation="PAPRLEALTGDWTRALVAATLVAIVLFGGYLRLTHLNWDSNGNG
                     ISGHLHPDERFLTQIAGDTRAPSSIANYFDTKTSALNPYNITHPDGSKQTDFVYGTLP
                     LFMNKFVASHLHVLSLGMLSNYDDYDGYNLAGRGLSAVIDIATIVFVFLLARELVDRR
                     AGLLAAFLYALAPFAIQNAHFFIVDPYVAFFATASLLFAVRVAKYGYRRDMALAGVAA
                     GLATACKITAVSMLPVIVLAIGVYCWPAVKPYVAPWWYGKSKQYRAQQDGRRLDAAVA
                     NFVIGSLIALVTAFVAFRIAMPYAFQTPSIGQLFSWNLGSFGPIPVPYPDMMNHHWLQ
                     NEVDQHNLLSGAAFPPNVQWIARSKWLWPLQQIVDWGMGPALGITAWLGVLFAIVFAF
                     VKRRGVWLVPLAWVIGYFAFMGAQFSLYMRYFLPLYPALAVFAAALLWHAAQWASSAD
                     P"
BASE COUNT          254 a          423 c          493 g          185 t
ORIGIN      
        1 cgggtccgcg gacgacgccc actgcgcggc gtgccagagc agcgcggcgg cgaacacggc
       61 aagcgcgggg tacagcggca ggaaataccg catgtagagc gagaactgcg cacccatgaa
      121 cgcgaagtag ccgatgaccc aggcgagcgg cacgagccag acgccgcgcc gcttcacgaa
      181 cgcgaacacg atcgcgaaca gcacgccgag ccaggcggtg atgccgagcg cggggcccat
      241 gccccagtcc acgatctgct gcagcggcca cagccacttg gagcgggcga tccactgcac
      301 gttcggcggg aacgccgcgc cggacagcag attgtgctga tcgacttcgt tctgcagcca
      361 gtggtggttc atcatgtccg ggtacggcac cgggatggga ccgaaggacc cgaggttcca
      421 cgagaacaac tggccgatac tcggcgtctg gaacgcatac ggcatcgcga tgcggaacgc
      481 gacgaacgcg gtgacgagcg cgatcagcga gccgatcacg aagttcgcga cggcggcatc
      541 gaggcggcgg ccgtcctgct gcgcgcggta ctgcttcgac ttcccgtacc accacggcgc
      601 gacgtacggc ttcacggccg gccagcagta gacgccgatc gccagcacga tcacggggag
      661 catcgacacg gccgtgatct tgcacgcggt cgccaggccc gccgccacgc cggccagcgc
      721 catgtcccga cggtagccgt acttcgccac gcgcacggcg aacaagaggg acgccgtcgc
      781 gaagaacgcg acgtacgggt cgacgatgaa gaagtgcgcg ttctggatcg cgaacggcgc
      841 gagcgcatag aggaacgcgg cgagcaggcc ggcgcggcgg tctaccagct cgcgcgccag
      901 caggaacacg aagacgatcg tcgcgatgtc gatgacggcg gagaggccgc ggccggccag
      961 gttgtagccg tcgtagtcgt cgtagttgct gagcatgccc agtgagagca cgtgaaggtg
     1021 ggacgccacg aacttgttca tgaagagcgg cagcgtcccg tagacaaagt ccgtctgctt
     1081 cgagccgtcg gggtgcgtga tgttgtacgg gttgagcgcg gacgtcttcg tgtcgaagta
     1141 gttcgcgatg ctgctcggcg cgcgcgtgtc gccggcaatc tgcgtgagga agcgctcgtc
     1201 cgggtggagg tggccgctga tgccgttgcc gttgctgtcc cagttgaggt gggtcaggcg
     1261 caggtagccg ccgaagagca cgatcgcgac gagcgtggcg gcgacgagcg cccgcgtcca
     1321 gtcgccggtc agcgcttcga gtcgcggggc gggga
//
LOCUS       DASUCJ010000020         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_550406, whole genome shotgun sequence.
ACCESSION   DASUCJ010000020 DASUCJ010000000
VERSION     DASUCJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550406"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1083
                     /locus_tag="VFU90_00230"
     CDS             <1..>1083
                     /locus_tag="VFU90_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5478220.1"
                     /translation="HTRPAVWYAVSAALILLGALTKSAQFPFHFWLPHAMTAPTPVSA
                     YLHSAAMVKLGVFLMARLWPVLSGTDAWIWIVGSAGLVTFCFGAYAAIFQQDLKGLLA
                     YSTISHLGLITLLLGLNSPLACVAAIFHMLNHTTFKASLFMATGIIDHEAGTRDMRRL
                     SGLYRFMPITATLATVAAAAMAGVPLLNGFLSKEMFFAEALDVEGPLPSLDRALPYLV
                     TVAAMFSVTYSVRFIYGVFLGPDPVGLPREPREPPRWMRLPIDVLVITCVVVGVFPAQ
                     TIGPLLGVAVASVLGDATPAYRLAPWHGFTLPLLMSAVALAGGLALFVGLRRHFARGD
                     QHTPFLPPIDARRVFDIALVMLSWRWA"
BASE COUNT          139 a          370 c          351 g          223 t
ORIGIN      
        1 cacacacggc ccgcggtctg gtacgcggtc agcgccgcgc tgatcctgct cggcgcgctg
       61 acgaaaagcg cgcagtttcc tttccacttc tggctgccgc atgcgatgac tgcgccgacg
      121 cctgtgtcgg cgtacctgca ctccgccgcc atggtgaagc tgggcgtgtt tctgatggca
      181 cgattgtggc cggtgctgtc gggcaccgac gcgtggatct ggatcgttgg gagcgccgga
      241 ctggtcacgt tctgtttcgg cgcttacgcc gcgatcttcc agcaggacct caagggcctg
      301 ctcgcgtact ctacgatcag ccaccttggg ctgatcacgc tgctgctggg attgaacagc
      361 ccgctcgcct gcgtggcggc gatcttccac atgttgaacc atacgacgtt caaggcgtcg
      421 ctgttcatgg ccaccggcat catcgaccac gaggcgggaa cgcgcgacat gcgtcggcta
      481 tccggtctct atcgattcat gccgatcacg gcgacgctcg cgacggtcgc cgccgcggcg
      541 atggccggcg tgccgctgct gaacgggttt ctctcgaagg agatgttctt cgccgaggcg
      601 ctcgacgtcg agggaccgct gccgtcgctc gaccgcgctc tgccgtacct cgtgaccgta
      661 gcggcgatgt tcagcgtaac gtattcggta cggttcatct acggcgtgtt cctcggtccc
      721 gatccggtcg gtttgccgcg cgagcctcgc gagccaccgc ggtggatgcg cctgcccatc
      781 gacgtgctcg tgatcacctg cgtcgtcgtc ggcgtatttc cagcgcagac gatcggcccc
      841 cttctcgggg tcgcggtcgc gtccgtgctc ggcgacgcga cccctgccta ccgcctcgcg
      901 ccgtggcacg gattcacgct gccgctgctg atgagcgcag tcgcgctcgc agggggcctg
      961 gcgctgttcg tcggcctgcg caggcatttc gcccgcggcg accagcacac accgttcctt
     1021 ccgccgatcg acgcacgccg cgtgttcgac attgcgctgg tcatgctgtc gtggcgttgg
     1081 gcg
//
LOCUS       DASUCJ010000021         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1131074, whole genome shotgun sequence.
ACCESSION   DASUCJ010000021 DASUCJ010000000
VERSION     DASUCJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131074"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..>1173
                     /locus_tag="VFU90_00235"
     CDS             321..>1173
                     /locus_tag="VFU90_00235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEU5478221.1"
                     /translation="MLGHELRNPLAAATNAVSLLDLSDLDAQDAQQAREIAQRQLQNM
                     TRLIDDLLDVARVTRGKVTLHREAVDLTELVRRVSAAIRPLIDSHRQSVELALPAERL
                     RVEGDPTRLEQILNNLLNNATKYTEAGGRIRLALERRGADAEIRVSDDGLGMGPDTLA
                     RAFDLFAQAERSLDRSQGGLGIGLTMVKSLVALHGGQVEARSDGLGQGSEFVVRLPAL
                     QPDADDAPLADSQETKLFSNNSPAPGVLLVEDNRDAAQALLLLLKHLGYDVRAVHDGF
                     EALEVAVD"
BASE COUNT          217 a          381 c          392 g          183 t
ORIGIN      
        1 gctggtacga gttcaccggg ctctgcgagg ggacgcaaga cgacgacaaa tggccgacga
       61 ttctgcaccc cgacgacgcc gaacgctcgg tcgccgcctg gcatgcggcg ctagcttcgg
      121 gcgacgccta cgagaacgaa tatcgctgcc aggatcggcg cacgggtgaa tatcgctggc
      181 acctggtccg ggcgctgccg gcgcgcgact cggcggggcg gatcatccgc tggttcggca
      241 cgctgaccga catccacgaa ttgaagcagg cgcaagccgc gctcgaagag tcgagccgcc
      301 gcaaagacga attcctggcg atgctcgggc atgaattgcg caaccccttg gcggccgcga
      361 cgaacgccgt gagtttgctc gatctgtccg acctcgatgc gcaagacgcg cagcaggccc
      421 gcgaaatcgc ccagcggcag ttacagaata tgacccggct gatcgacgac ttgctcgacg
      481 tggctcgcgt cacgcgcggc aaggtcacgc tgcaccgcga agccgtcgat ctgacggagc
      541 tcgtccgccg agtttcggcg gccatccggc cgctgatcga ttcgcaccgg caaagcgtcg
      601 aacttgctct gccggccgag cgtctgcggg tggagggcga tccgactcgc ttggagcaga
      661 tcctgaacaa cctattgaac aacgccacca aatacaccga ggctggcgga cgcattcgcc
      721 tggctctcga gcgacgcggc gccgacgcgg aaattcgcgt ttcggacgac ggcctgggca
      781 tggggcccga tacgctggcg cgagccttcg acctgttcgc ccaggccgaa cgctcgctgg
      841 accgttcgca aggcgggctg gggatcgggc tgacgatggt caaaagcctc gtcgccttgc
      901 acggaggaca ggtcgaggcg cgcagcgacg gcttgggcca aggcagcgaa ttcgtcgtcc
      961 gcctgcccgc cttgcaaccc gacgcggacg acgcgccgct cgccgattcg caagaaacga
     1021 agctgttcag caacaactcg ccggcgccgg gcgtgctgtt ggtggaagac aaccgcgacg
     1081 cggcgcaggc cttgctgctg ttgctcaagc atttgggata cgatgtacgg gccgtacacg
     1141 acggcttcga ggcgctcgaa gtcgccgtcg att
//
LOCUS       DASUCJ010000022         1185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_474019, whole genome shotgun sequence.
ACCESSION   DASUCJ010000022 DASUCJ010000000
VERSION     DASUCJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474019"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VFU90_00240"
     CDS             <1..1125
                     /locus_tag="VFU90_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478222.1"
                     /translation="RRKPNGKYIEISNARKHNLKNLDIRIPLGMMVCITGVSGSGKST
                     LVNEVLVRALSQHLHGQPPGGTYGTVKGAAQLDKLVVIDQSPIGRTPRSNPATYTGTF
                     DYIRELFSKVPEARMRGYAPGRFSFNVKGGRCEACQGDGIIKIEMHFLPDVYVPCEVC
                     KGKRYNAQTLEIKFKGKTISDVLEMRVDEANAFFENIPRVHNKLRTICDVGLGYIKMG
                     QPATTLSGGEAQRVKLATELSRRSTGRTFYVLDEPTTGLHFADVHRLLEVLQRLVSLG
                     NTVLVIEHNLDVIKTADYLIDLGPEGGDRGGTVVATGTPEAVAADPASYTGHYLAPVL
                     RDQRAVGHHVPDAAELERLERENLAVLDDLAKGERVAVEA"
BASE COUNT          236 a          392 c          379 g          178 t
ORIGIN      
        1 cgacggaaac ccaatgggaa gtacatcgaa atctccaacg cacgcaaaca caatctcaag
       61 aacctggaca tccgcatccc gctcggaatg atggtgtgca taaccggggt gagcggaagc
      121 gggaaatcga cgctcgtcaa cgaggtgctg gtccgcgcgc tcagccagca tcttcacggc
      181 cagccgcccg gcggaacgta cggcacggtc aagggcgcgg cccagctcga caaactcgtg
      241 gtgatcgatc aatcgccgat cggacgcacc ccgcgcagca acccggcgac gtataccgga
      301 acgttcgatt acatccgcga gctcttttcg aaggttcccg aagcgcggat gcgcggctac
      361 gcgcccgggc ggttttcgtt caacgtcaaa ggcggccgct gcgaggcctg ccagggcgac
      421 ggcatcatca aaatcgagat gcacttcctg cccgacgtct acgtcccgtg cgaagtctgc
      481 aagggcaagc gctacaacgc ccagacgctc gagatcaagt tcaaagggaa gacgatttcg
      541 gacgtgctcg agatgcgggt cgacgaagcc aacgccttct tcgagaacat cccgcgcgtg
      601 cacaacaagc tcagaacgat ttgcgacgtc ggtctgggct acatcaaaat gggccagccc
      661 gccaccaccc tctcgggcgg cgaggcgcag cgcgtcaagc ttgccaccga gctgtcgcgc
      721 cggtcgaccg gacgcacgtt ttacgtgctc gacgagccga ctaccgggct gcattttgcc
      781 gacgttcacc ggctgctgga ggtgctgcag cggctcgtgt cgctcggcaa cacggtgctg
      841 gtgatcgagc acaacctcga cgtgatcaaa accgccgact atttgatcga tctcggcccc
      901 gagggcggcg atcgcggcgg aacggtggtg gccaccggca cgcccgaagc ggtggcggcc
      961 gacccggctt cgtataccgg gcattacttg gcaccggtgc tgcgcgatca gcgcgcggtc
     1021 gggcaccacg ttccggacgc cgccgagctc gagcggctcg agcgcgagaa cctcgcggtg
     1081 ctcgacgacc tggcgaaggg cgagcgcgtc gccgtcgaag cctagcggca tgcgcgccgc
     1141 cgccgcgcgc gccgcggagc tgcgcgcgct gatcgacgaa gccaa
//
LOCUS       DASUCJ010000023         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1329870, whole genome shotgun sequence.
ACCESSION   DASUCJ010000023 DASUCJ010000000
VERSION     DASUCJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329870"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..580)
                     /locus_tag="VFU90_00245"
     CDS             complement(77..580)
                     /locus_tag="VFU90_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478223.1"
                     /translation="MSALSPRGLRETLRLMPVLVLDAAVIVASYAAALALRFDGDIPE
                     KSLQFFALASPFIVAAYLLGNIFFRIYRTSWKYAGIVDALNIALSIGVVSIFLFAINA
                     FLSPRHIPLTVNVVAPALMLIAMGGIKFWPRLWASRNPFAGYDAGVKDVLIVGAGHTG
                     QLLAREL"
     gene            complement(722..1312)
                     /locus_tag="VFU90_00250"
     CDS             complement(722..1312)
                     /locus_tag="VFU90_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5478224.1"
                     /translation="MLDRAANGLMGLAGLIVFVTTYPLIALAIKIDSRGPVIFSQRRV
                     GLKGRTFTFYKYRTMSHEGHKPYETPKLDAEHFRTFVFITPVSRLTRVGRFLRATSLD
                     ELPNFWNVLKGDMRIVGPRPEIPELVSQYPPEYHRRHDVKPGITGLAQVSGRADLTYE
                     ETMNYDLDYVNNHSLARDFTILMKTLPAVIARKGAR"
     gene            complement(1305..>1661)
                     /locus_tag="VFU90_00255"
     CDS             complement(1305..>1661)
                     /locus_tag="VFU90_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5478225.1"
                     /translation="LPEIIVPSEREGTTHAWHLYVVRLRPELLEIGRDRFIEELRACG
                     IGTSVHFIPVHHHPYYREAYGWAPGDFPRADAAFETMLSLPLYTRMTAETVERVADAV
                     EDVVRGHRRAGGTMDA"
BASE COUNT          283 a          578 c          543 g          257 t
ORIGIN      
        1 gatcgacgcc gagctgtgcg tggcggcgct gcgcagccgg tcggtgctga tcaccggcgc
       61 cgccggctac atcgcatcag agctcgcgcg ccagcagctg gcccgtgtgc cccgcgccga
      121 cgatcagcac atccttcacg ccggcgtcgt accccgcgaa cgggttgcgc gaagcccaga
      181 gccgcggcca gaacttgatc ccgcccatcg cgatcagcat cagcgccggc gcgacgacgt
      241 tcaccgtcag cgggatgtgg cgcgggctga ggaacgcgtt gatcgcgaag aggaagatcg
      301 acacgacgcc gatcgacagc gcgatattca gcgcgtcgac gatgccggca tacttccacg
      361 acgtgcggta gatgcggaag aagatgttgc ccagcaggta cgccgcgacg ataaacggcg
      421 acgcgagggc gaagaactgg agcgacttct ccgggatgtc gccatcgaag cggagcgcca
      481 gcgccgccgc gtacgacgcg acgatgaccg ccgcgtcgag caccagcacc ggcatgagac
      541 gcagcgtctc acggaggccg cgcgggctca gcgcgctcac gatgcgcccg cgagcgtcac
      601 gcgcgatgcg ggaaagcggc ttcgggggat acggtgcgcg cgcggcatag cgtgcgggat
      661 gtcctgcgtg gcgtcgcctc cctgcccgcc aggggccgcc cggggtgggc gagcaacggc
      721 gctagcgagc gcccttgcgt gcaatcaccg ccggcagcgt tttcatgaga atcgtgaaat
      781 cgcgtgccag cgaatggttg ttcacgtaat cgagatcgta gttcatcgtt tcttcgtacg
      841 tcaaatccgc ccgcccgctc acctgcgcga ggcccgtgat gcccggcttc acgtcgtgac
      901 ggcggtggta ctccggcggg tactgcgaca ccagctccgg gatctccggc cgcggcccga
      961 cgatgcgcat gtcgcccttc agcacgttcc agaagttcgg cagctcgtcg aggctcgtcg
     1021 cgcgcaggaa ccgcccgacg cgcgtcagcc gcgacaccgg cgtgatgaac acgaacgtcc
     1081 ggaagtgctc cgcgtcgagc ttcggcgtct cgtacggctt gtgcccttcg tgcgacatcg
     1141 tccggtactt gtagaaggtg aacgtgcgcc ccttcagtcc gacgcgcctc tgcgagaaga
     1201 tcaccgggcc ccgcgagtcg atcttgatcg ccagcgcgat cagcggatac gtcgtcacga
     1261 acacgatcag tcccgcgagc cccatcagcc cgttcgccgc ccggtcaagc atccatcgtc
     1321 cccccggccc tccggtgccc gcgcacgacg tcttccacgg cgtccgcgac gcgctcgacg
     1381 gtctccgcgg tcatacgtgt gtacaacggc agcgacagca tcgtctcgaa tgccgcgtcg
     1441 gcgcgcggga agtcccccgg cgcccagccg tatgcctctc gataatacgg gtgatggtgc
     1501 accgggataa agtgcaccga cgtgccgatc ccgcacgcgc gcagctcttc gatgaacctg
     1561 tcgcgcccga tttcaagcaa ttccggccgt agccggacca catacaggtg ccacgcgtgc
     1621 gtcgtgccct cgcgctcgga cggcacgatg atctcgggca g
//
LOCUS       DASUCJ010000024         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_718589, whole genome shotgun sequence.
ACCESSION   DASUCJ010000024 DASUCJ010000000
VERSION     DASUCJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718589"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFU90_00260"
     CDS             <1..365
                     /locus_tag="VFU90_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478226.1"
                     /translation="VGEYNKVMSFRRANWTLAAYFRTGYLYETFSKALLNAPCPPEVK
                     RLGADACDIYRDQIEQTVAGVDEEAVKRYGVTLQKAGEFGVSNEWTKLARTRANAYKP
                     DVFPMTKDERIDMQLESP"
     gene            431..>1102
                     /locus_tag="VFU90_00265"
     CDS             431..>1102
                     /locus_tag="VFU90_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5478227.1"
                     /translation="MVCAVGCQLSAVSFAAQTQLPAETKGDPKPDTLAAQNYLKRQRY
                     EDAIRQAKLALGRDERYVPAMVVLAKAYYFERKYELATSIVDMAKSIDPNNAECYNLL
                     GFIALTHDDRISATAAFRKATELDSRYGNAWDNLAAQYLYGKNYDGALDAAKKATDLM
                     PQFATAWNNLGSAYRGKQQYAEAESAYKRATQLDPNYANAYFDLGILYLDAKQMPGTD
                     LITKLN"
BASE COUNT          222 a          358 c          353 g          169 t
ORIGIN      
        1 tcgtcggcga gtacaacaag gtgatgtcgt tccgtcgggc aaactggacg ctcgccgcat
       61 atttccgtac cggctacctc tacgagacgt tctcgaaggc gctcttgaac gcgccgtgcc
      121 cgccggaggt caagcgattg ggcgcggacg cgtgcgacat ctatcgcgat cagatcgaac
      181 agaccgtggc aggcgttgat gaggaggcgg tgaaacgcta tggcgtcacg ttgcagaagg
      241 ccggcgagtt cggcgtgtcc aacgagtgga ccaagctcgc ccgcacgcgc gccaacgcgt
      301 acaagccgga tgtgttcccg atgacgaagg acgagcgcat cgacatgcag ctggagtcac
      361 cgtgagaaag cagccgtcag ccgtcagccg tcagccgtca gcccggagtc ggatgcgtgt
      421 ggcgctgttc gtggtctgtg ctgttggctg tcagctgtcg gcggtcagct tcgcggcgca
      481 gacgcagttg ccggcggaga ccaaggggga tccgaagccg gacacgttgg ccgcgcagaa
      541 ctacttgaag cggcagcgct acgaggacgc gatccgccag gccaagctcg cgctcggccg
      601 cgacgagcgt tacgtgccgg cgatggtcgt gctggccaag gcctactact tcgagcgcaa
      661 gtacgagctg gcgacgagca tcgtcgacat ggccaagtcg atcgatccca acaacgccga
      721 gtgctacaac ctgctcgggt tcatcgccct cacgcacgac gatcgcatct cggcgacggc
      781 cgcgttccgc aaggcgaccg agctcgactc gcgctacggc aacgcctggg acaacctggc
      841 cgcgcagtac ctctacggca agaactacga cggcgcgctc gacgcggcga agaaggccac
      901 cgacctcatg ccgcagttcg cgacggcgtg gaacaacctc ggctccgcgt accgcggcaa
      961 gcagcagtac gccgaggccg agagcgccta caagcgcgcc acgcagctcg atccgaatta
     1021 cgccaacgcg tacttcgatc tcgggatcct gtatctcgac gccaagcaga tgccgggcac
     1081 cgacctcatc accaagctca ac
//
LOCUS       DASUCJ010000025         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_474069, whole genome shotgun sequence.
ACCESSION   DASUCJ010000025 DASUCJ010000000
VERSION     DASUCJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474069"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFU90_00270"
     CDS             complement(<1..803)
                     /locus_tag="VFU90_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5478228.1"
                     /translation="MAGLAYCDADGRIYFDESTVPLADGGIVRCVESGELIPAPAGTV
                     EMILPGRRPLTSVGPASDRYALAAVLPAGYTRLLVPACVKDRDAPDLPLFGYTFACVA
                     GDRLHVAAMKTDEGEDWQPRYFAAGELEALLDRRVADDPGNRVLRQVARCSLEYGCFT
                     AQNVFLERGEAALPVSPKCNARCIGCISEQEPDAQLPSPQERIVFETAAGELTRIAVR
                     HLERVADGIVSFGQGCEGEPLLRSTTIARAIGEIRAACSNGTINLNTNG"
     gene            complement(811..1575)
                     /gene="dapF"
                     /locus_tag="VFU90_00275"
     CDS             complement(811..1575)
                     /gene="dapF"
                     /locus_tag="VFU90_00275"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5478229.1"
                     /translation="MHGACNDFVVIDRRSQRVESLNEFARWACNRREGIGADGLIAIE
                     KTASSGLAMRTLNADGSEAEMCGNGIRCAARWLDEAGEGDRLEFATPAGIVRTQVVVR
                     RPEYVVKVEMGVPKILTVDSGDAVLVDMGNPHVVLFREDVDGFDLPAAAQRFARDPAL
                     PQGANVHVASVEGGGRLRVRHWERGVGETPACGTGAVACAAAAIAEDRATAPVDVLVP
                     GGRLIVEWDGAGRATLTGPAVRVFDTEVPAAPFAER"
BASE COUNT          293 a          619 c          545 g          284 t
ORIGIN      
        1 ctgccgttgg tgttgagatt gatcgtgccg ttgctgcaag cggcacggat ctcgccgatc
       61 gctcgggcga tcgtcgtcga acgcagcagc ggctcgcctt cgcagccttg cccgaacgag
      121 acgatcccgt cggccacgcg ctcgagatgg cggacggcga tgcgcgtaag ctcgccggcc
      181 gccgtttcga agacgatccg ttcctgcggt gagggaagct gcgcgtccgg ctcctgctcc
      241 gaaatgcagc cgatgcagcg ggcgttgcat ttgggcgaaa ccggaagcgc cgcttcaccg
      301 cgttcgagaa agacgttctg cgcggtaaag cagccgtact ccagcgaaca gcgcgctacc
      361 tgacgcaaca cccggtttcc gggatcgtcc gcaacgcggc gatcgagcag cgcttcgagt
      421 tctcccgcag cgaagtaacg cggctgccag tcttcgcctt cgtcggtttt catggcggcg
      481 acgtgcagcc ggtcgccggc gacgcaggcg aacgtgtagc cgaacagcgg gaggtcgggc
      541 gcgtcgcgat ccttgacgca ggccggaacg agcaaccgcg tatagccggc cggtaatacc
      601 gcggccagcg catagcgatc gctcgcgggc ccgacgctcg tcaacggccg ccggccaggc
      661 aaaatcatct cgacggtccc cgccggggcg gggatcagtt cgccgctctc gacgcaacgg
      721 acgatgccgc cgtcggccag cggcacggtg gattcgtcga agtagatgcg cccgtcggca
      781 tcgcagtatg ccagccccgc catcgtgcgg tcagcgttcg gcgaacggag cggccggaac
      841 ttcggtatcg aaaacccgta cggccgggcc ggtcagcgtc gcccgtccgg caccgtccca
      901 ttcgacgatc aagcggccgc cgggcaccag cacgtcgacc ggcgccgtgg cgcgatcttc
      961 cgctatcgca gccgccgcgc atgccaccgc acccgtcccg cacgccggcg tttccccgac
     1021 gccgcgctcc cagtgccgca cgcgcaaacg gccgccgccc tcaaccgatg cgacgtgaac
     1081 gttcgcaccc tgcggcaaag cgggatcgcg cgcaaagcgc tgcgccgcgg ccggcagatc
     1141 gaaaccatcg acgtcctccc gaaagagcac gacgtgcgga ttacccatgt cgaccagcac
     1201 cgcatcgcca gagtcgaccg tgaggatctt cgggacgccc atctccactt taacgacgta
     1261 ttccggccgg cgaacgacga cctgcgttcg cacgattccc gccggcgtag cgaactccaa
     1321 gcgatcgcct tcgccggcct cgtcgagcca gcgtgccgcg caacggatgc cgtttccgca
     1381 catttccgcc tcgctgccgt cggcgttcag cgtgcgcatt gcgagaccgg acgaagccgt
     1441 tttttcgatc gcgatcagac cgtcggcacc gatcccttcg cgccggttgc acgcccaacg
     1501 cgcgaattcg ttcagcgatt cgactcgctg gctccggcgg tcgatgacga cgaaatcgtt
     1561 gcacgcaccg tgcattttcg taacggcaac ccgcacgctc acccgtcttc ggctgcgccg
     1621 gccgcggtct cgtttgcgtc cggctcttcg acgccgacct gcgttcccgg agaaatcgtc
     1681 gagatcgcgt gcttatacac gagatgcgtc ttgcgttcgt actccagcag cacggtaaac
     1741 g
//
LOCUS       DASUCJ010000026         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_122532, whole genome shotgun sequence.
ACCESSION   DASUCJ010000026 DASUCJ010000000
VERSION     DASUCJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122532"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1270
                     /locus_tag="VFU90_00280"
     CDS             143..1270
                     /locus_tag="VFU90_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478230.1"
                     /translation="MMHSARLLTMGAIALAVAAVTVPALGQTTGVEAELIHAAPPSST
                     ITPAQRARLAEKLRLVDQIVGVAAETNDGSAVTPEQLRWLRESLYMLPLAEVQAIGIP
                     GGFRSAATAVSSTQRSPRVAKALGDPTTDLVYRPITPCRYIDTRVKPPMVGSTPYGVD
                     LGQTGASYGGSAGCNIVTAAGLTDASDIAAVNMNVGIVNPQFQPGFLGARPAGSANST
                     SMVNWYQAGPTVQASNAGAVTTSGAATGSNQIEFFGTPTDLVVDVLGVFARPGATPFN
                     CNFAQLDGTGTGNVAIGAQFTIPSPPQACQTGFTPVGLGCTYGPVAPAGLSLVSKSIA
                     VAPTDFPQPGFASCAWVNGSTTALNGSDFHAITRCCQVPGR"
BASE COUNT          213 a          459 c          405 g          230 t
ORIGIN      
        1 ccttcacgcg acgcagagcg agagcctgcg tcgcgccatt tcggcagtct ggcgcgttgt
       61 tcctgcgcgc attgcgatcg ggaacgaaac ttgctgccgg acgatctccg tgcggctttc
      121 ccatacttcc tggagttccc cgatgatgca ttcggcgcgt ttgctgacga tgggcgcaat
      181 cgcgctcgcg gttgcggcgg tcactgttcc ggcacttggc cagacaaccg gcgtggaggc
      241 tgagctgatc catgccgcac ccccctcgag cacgatcacg cccgcacagc gtgcacgact
      301 cgccgaaaag cttcgccttg ttgaccagat cgtcggcgtc gccgccgaaa ccaatgacgg
      361 ctcggccgtc acaccggaac aactccgatg gctacgcgag tcgctctaca tgctcccgct
      421 cgccgaagtc caggcgatcg gcattccggg cggcttccgt tcggccgcca ccgcggtctc
      481 ctcgacgcaa cgcagcccgc gcgtcgcgaa ggcgctcggc gatccgacga ccgatctcgt
      541 ctacaggccg atcacgccat gccgctacat tgatacccgg gtgaagccgc cgatggtcgg
      601 ctccacgccc tacggcgtcg acctcggcca gacgggcgcg agctacggcg gctcggccgg
      661 atgcaacatc gtaaccgcgg ccgggctcac cgacgcatcc gatatcgccg ccgtcaacat
      721 gaacgtcggc atcgtcaatc cgcagttcca gccgggcttt ctcggcgcgc gaccggccgg
      781 ctcggcgaac tcgacgtcga tggtcaactg gtatcaggcc ggtccgaccg tgcaggcctc
      841 gaacgccggc gccgtgacga cttcaggagc cgcgacggga agcaaccaga tcgagttctt
      901 cggaacgcca accgatctcg tggtcgacgt cttaggtgtg ttcgcgcggc ccggcgcgac
      961 accgttcaat tgcaatttcg cgcaactcga tggcaccggt accggtaacg ttgcgatcgg
     1021 agcgcaattc acgatcccca gcccgccgca ggcctgccag accgggttca cgccggtcgg
     1081 gcttggatgc acctatggcc cggttgctcc ggctggcctc tcgctggtat cgaagagtat
     1141 tgcggttgca ccgaccgatt tcccacagcc cggcttcgcg agttgcgcct gggtgaacgg
     1201 ttcaacgacc gcgctgaacg gcagcgattt ccacgcgatc acgcgctgct gccaggttcc
     1261 tggccgctag cgtgagcggc gccaggtttg acgtgcgccg cggccgc
//
LOCUS       DASUCJ010000027         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1116041, whole genome shotgun sequence.
ACCESSION   DASUCJ010000027 DASUCJ010000000
VERSION     DASUCJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116041"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFU90_00285"
     CDS             complement(<1..504)
                     /locus_tag="VFU90_00285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEU5478231.1"
                     /translation="MAAQLIVALDVPSPAQAERLIDQLYELDVIFKIGYEALYGYPER
                     IFAYCEARDVRAFVDAKLHDIPRTVEAAVRHLVRPFVHILNVHALGGNEMMRAAVESA
                     RERAGELGVQPPLVFAVTILTSIAPEDLNELGLQGGPGENATRLAALARDAGCAGVIC
                     SAHEVPDL"
     gene            complement(525..1487)
                     /locus_tag="VFU90_00290"
     CDS             complement(525..1487)
                     /locus_tag="VFU90_00290"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU5478232.1"
                     /translation="MLSAQPALAVDVGGLKLAFPTLMGSGCYGSGEEFAPFLDQSSLG
                     AVVLKSVTRLPRLGNPTPRLVRTPAGLLNAIGLQNPGIEWYVEHDLPKYAGRPYPLVG
                     SVAGFSVDDYAYVCERLAACDPIAAIELNISCPNVASEGETFACDPSLTGRVVRAARA
                     TTDKPLIVKLSPNVTDIRAIAYEAQEAGADALAVVNTVRGMGIDVETWRPRLGNVTGG
                     LSGPAIRPIAVLAVYEVARAVTIPIVGQGGIETVTDALEFFLAGATAIGIGTANFTDP
                     RVPERIAEGLRAYLRRRGLSAIGEIVGKANVGFANADQYEGDEG"
BASE COUNT          251 a          502 c          491 g          256 t
ORIGIN      
        1 gagatcgggc acttcgtgcg cgctgcagat gacgccggcg cagccggcgt cgcgcgcgag
       61 tgccgccaaa cgcgttgcgt tttcgcccgg accgccttgc agcccgagct cgttgagatc
      121 ctccggcgcg atgctcgtga gaatcgtcac cgcgaacacc agcggcggtt ggacgcccag
      181 ctcgccggcc cgttcgcggg cgctctcgac cgccgcgcgc atcatctcgt ttccgcccag
      241 cgcgtggacg ttcagaatgt gcacgaacgg acgcacgaga tggcgaacgg ccgcctctac
      301 cgtgcgcggg atgtcgtgga gcttggcgtc gacgaacgcg cgtacgtcgc gcgcctcgca
      361 gtacgcgaag atccgctccg gatatccgta cagcgcttcg tacccgatct tgaagatgac
      421 gtccagctcg tagagctggt cgatgagccg ttcggcttgt gcgggtgagg gaacgtcgag
      481 cgcgacgatt agttgtgcgg ccatcgtgcg actactatag agcgttagcc ctcgtctcct
      541 tcatattgat cggcgttcgc gaatccgacg tttgccttgc cgacgatttc gccgatcgcc
      601 gacaagccgc ggcgccgcaa gtacgcgcgc aatccttcgg cgatgcgctc cggaacgcgc
      661 ggatcggtga agttggcggt gccgataccg atggcggtcg cgcccgccaa gaagaactcg
      721 agcgcgtcgg tcacggtttc gattccgcct tgacccacga tcggaatcgt caccgcgcgc
      781 gctacctcgt aaaccgcgag cacggcgatc ggccgaatcg cgggtccgga gaggccgccg
      841 gtgacgttgc ccaagcgcgg gcgccacgtt tccacgtcga tccccatccc gcgcaccgtg
      901 ttgacgacgg ccagcgcatc ggcgccggct tcctgcgctt cgtacgcaat cgcccggatg
      961 tcggtgacgt tgggcgagag cttgacgatc agcggcttgt cggtggtcgc gcgcgccgcg
     1021 cgcacgacgc ggccggtcag gctcggatcg caagcaaagg tctctccttc gctcgccacg
     1081 ttcgggcacg agatgttcag ctcgatggcg gcgatcggat cgcacgcggc cagccgttcg
     1141 cacacgtatg cgtagtcgtc gaccgaaaaa ccggcgacgc tgccgaccag cggatacggt
     1201 cgccccgcat atttcgggag atcgtgctcg acgtaccact cgatcccggg attctgcagg
     1261 ccgatggcgt tgagcaggcc ggcgggcgta cgaactaagc gcggcgtcgg attgcccagg
     1321 cgcggcagcc gggtaacgct cttgagcacc accgctccaa gcgacgactg atcgaggaac
     1381 ggagcgaact cctcgccgga tccataacag ccgctgccca tcagcgtcgg aaacgcgagc
     1441 ttcaggcctc cgacgtcgac ggcgagagcc ggttgcgcgc tcagcacgtt accatctcag
//
LOCUS       DASUCJ010000028         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1207743, whole genome shotgun sequence.
ACCESSION   DASUCJ010000028 DASUCJ010000000
VERSION     DASUCJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207743"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFU90_00295"
     CDS             <1..409
                     /locus_tag="VFU90_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478233.1"
                     /translation="VATVHPKYRTPFVALLLYMVLGLIGVTTRSFEQLAEMYVLGIWP
                     FYALAVGAVFLVPRRRPDLASLCRAWGYPVLPAAFILVSIAMVAGGAVTRPLETGVGV
                     AIILLGVPVYYAWRALGKRRAAAERAASHSVTR"
     gene            406..1569
                     /locus_tag="VFU90_00300"
     CDS             406..1569
                     /locus_tag="VFU90_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478234.1"
                     /translation="MIYFVVGAAGSFSIRDYLGEEGRALRDRVRVVLYEELARLRELP
                     LGTWVFTELDRLGAPERELVGIACEQLGAANAGVRLLNQPRHVRLRAELLRAASESGI
                     NRFRAWPATGVRLATTGRSSAGDVPAAALRYPVFVRFANRHVGSLTPLLGSPRALAEA
                     LASLVAGQRRLDELLVVEFCDTKDAHGIYRKYSAFNVGGRILPRYMECSRDWMVKWHH
                     RIFDRERAAMETEYLATNPHEAWIREMFCLAGIEYGRIDYGLLAGEPQMWEINLNPTI
                     GRGPGPRVAQPAEVVAYKAILAPAFDGFYARFREAWEAIDSTDGAGAVELRIPDRLRQ
                     AIERAVRQRRRDARLGAFVDAIARQRWLRPVTRPVKRALARVAGARLRRSRSS"
     gene            1654..3123
                     /locus_tag="VFU90_00305"
     CDS             1654..3123
                     /locus_tag="VFU90_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5478235.1"
                     /translation="MPNTRGGNSTRLTEPCGRRTFLSVCAAADAGLLAGVPLGSARPR
                     PTPAQLRYERRERALFLHFGVNTFTDREWGTGEESPSLFDPSALDARQWARSARSAGF
                     RSMVLTAKHHDGFCLWPSAVTDHGVRRSPWRGGAGDVVREFTDACRAEGLGAGLYLSP
                     WDRHERSYGDSPRYNDFYVAQLTELLTRYGPIAEVWFDGANGEGPNGKRQAYDWDRFH
                     RTVRRLQPDAVMFSDAGPDVRWIGNEDGVAGDPNWSIVDPLAVPFPGATGPAVERMLQ
                     HGDPTGTVWRPGEADVSIRPGWFWHAAESAKVKSVEELIDLFFVSVGRNANVLLNVPP
                     NRAGVLDEVDVARLASFSSELSAQFAENAAAGGRVSASSEASGHGAGATLDGDLDTWW
                     SPWGEDRLPSLAVTFRKPARIGVIELAEAIQNGQRVASHGVDALVAGRWQTISTGTTI
                     GHKKLDRVPPMTVQGVRLRIASSIGAPQIATIAVWPGRP"
     gene            3186..>3366
                     /locus_tag="VFU90_00310"
     CDS             3186..>3366
                     /locus_tag="VFU90_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478236.1"
                     /translation="MYRTPRVLRRVASPVALIASLALLAGCASVTPIRQLLDDPARYD
                     GKSVRIEGVVKGSAGA"
BASE COUNT          466 a         1101 c         1214 g          585 t
ORIGIN      
        1 ggtggcgacc gtgcacccga agtaccgtac gccgttcgtc gcgctgctcc tctacatggt
       61 gctcggcttg atcggtgtca cgacccgcag cttcgagcag ctcgccgaga tgtacgtgtt
      121 gggcatctgg ccgttctacg cgctcgccgt gggcgccgtg ttcctggttc cccggcgccg
      181 acccgacctc gcgtcgctct gccgcgcctg gggctatccg gtgctgcccg cggcattcat
      241 actggtgtcc atcgcgatgg tggcaggcgg cgcggtcact cgcccgctcg agacgggcgt
      301 cggcgtcgcg atcatcctgc tcggcgttcc ggtctactac gcgtggcgcg cgttaggcaa
      361 acgccgcgcg gccgcggagc gtgcggcgtc tcactcggtc acgcgatgat ctacttcgtc
      421 gtcggcgcag cgggcagctt ctcgatccgc gattatctcg gcgaggaggg ccgcgcgctg
      481 cgggaccgcg ttcgcgtggt cctgtacgag gagctcgcgc gcctgcgcga gctgccgctc
      541 ggcacttggg tcttcacgga gctcgaccgg ctcggtgcgc cggagcgcga gctcgtcggc
      601 atcgcgtgcg agcagctcgg tgctgcgaat gccggagttc ggctgctcaa tcagccgcgg
      661 catgttcggc ttcgtgcgga gctgctccgg gccgcgtccg agtcaggcat caaccggttt
      721 cgcgcgtggc ccgcgacggg cgttaggctt gcgaccaccg ggcgctcgag cgcgggcgac
      781 gtccccgcgg ccgcgcttcg ctatccggtg ttcgtgcgat tcgccaaccg gcatgtgggc
      841 agtctgacgc cgttgctcgg gtcgccgcgc gccctcgcgg aagcgctggc ctcgctcgtg
      901 gcagggcagc gccgcttgga cgagctgctg gtcgtcgagt tctgcgacac caaggacgcg
      961 cacggcatct atcgcaagta ctcggcgttc aacgtgggag ggcgcattct gccgcgatac
     1021 atggagtgca gtcgcgattg gatggtgaag tggcaccatc ggattttcga tcgcgagcgc
     1081 gcggccatgg agacggagta cctcgcgacg aatccgcacg aggcgtggat ccgcgagatg
     1141 ttttgcttgg cgggcatcga gtacggccgc atcgattacg gcctgctcgc cggggagccg
     1201 cagatgtggg agatcaattt gaaccccacg atcggtcggg ggcccgggcc gcgcgttgcg
     1261 caaccggccg aggtggtcgc gtacaaggcg atcctcgcgc ctgcattcga cggcttttac
     1321 gcgcggtttc gggaggcgtg ggaagcgatc gacagcacgg acggcgcggg cgccgtcgag
     1381 ctgcggattc cggaccggct tcggcaggcg atcgaacgcg cggttaggca gcggcgccgg
     1441 gacgcccggt tgggcgcctt cgtcgacgcg atcgcgcggc agcgctggct gcgtccggtg
     1501 acgcgcccgg tgaagcgcgc cttggcgcgt gtggcgggcg cgcggctgcg gcgcagtcgt
     1561 tcgtcgtgat ggtgcacggc gacaacccgc tcgcgaacga cgtgaggccg gattccggca
     1621 tcacgccgtc gcgcccgtag ccccgcggag ccgatgccga acacgcgcgg tggcaactcg
     1681 acgcgcctaa cggagccgtg cggccggcgc acgttcttgt cggtatgcgc ggcggccgac
     1741 gccgggctcc ttgccggcgt accgttaggc agcgcacgcc cgcgcccgac gccggcgcag
     1801 ctccggtacg agcgccgcga gcgcgccctc ttcctgcact tcggcgtcaa cacgttcacc
     1861 gaccgcgaat ggggcaccgg cgaggaaagc ccatcgctgt tcgacccgtc ggcgctcgac
     1921 gcccgccaat gggcgcgatc cgcgcgatcg gcgggctttc ggtcgatggt gctcaccgcc
     1981 aagcaccacg acggcttctg cctctggccc agcgccgtga cggaccacgg tgtgcgccgg
     2041 agtccgtggc gcggcggcgc gggcgatgtg gtgcgcgagt tcaccgatgc ctgtcgcgca
     2101 gagggcctgg gcgccggatt gtatctgtcg ccgtgggacc gccacgagcg atcgtacggc
     2161 gattcgcccc gctacaacga cttctacgtc gcccagctca cggagctgct gacgcgctac
     2221 ggaccgatcg ccgaagtctg gttcgacggc gccaacggtg aggggcctaa cggaaaacgc
     2281 caggcctacg actgggaccg gttccaccgc acggtgcgcc gtctgcagcc tgatgcggtc
     2341 atgttctccg acgccggccc cgacgtccgc tggatcggca acgaagacgg cgtggccggc
     2401 gatccaaact ggtcgatcgt ggatccgctc gccgtgccgt ttcccggcgc gacgggccct
     2461 gcggtggagc gaatgctgca acacggcgat ccgactggta cggtgtggcg acccggcgag
     2521 gccgacgtgt cgattcgtcc gggttggttc tggcatgcgg ccgagtccgc caaggtgaag
     2581 agcgtggaag aactgatcga cttgttcttt gtctccgttg gtcggaacgc caacgtgctg
     2641 ctcaacgtgc cgccgaatcg cgcgggtgtg ctggacgaag tcgacgtcgc tcgactcgcg
     2701 tccttctcga gtgaattgtc cgcccagttc gccgaaaacg ccgcggccgg cgggcgtgtg
     2761 tcggcgtctt ccgaggcatc cggtcatggt gcgggagcga cgctggacgg cgacctggat
     2821 acctggtgga gtccgtgggg cgaggatcga ttgccgtcgc tcgccgtgac gttccggaag
     2881 ccggcacgaa tcggtgtaat cgagttggcg gaagcgattc agaacgggca acgggtcgcg
     2941 tcgcacggcg tggatgcgct ggtggccggt agatggcaga cgatttccac cgggaccacc
     3001 atcgggcata agaagttgga ccgcgtgccg ccgatgacgg tccaaggcgt tcgccttcgg
     3061 atcgcgtcgt cgatcggcgc gccgcagatc gcgacgattg ccgtatggcc gggccgtccg
     3121 tgacgttgac ggcgcgcccg tgagcgttgg acacaatcga gatccttcag ccggagcatc
     3181 ccatcatgta tagaaccccc cgtgttcttc gccgcgtggc gtcgcccgta gcgctgatcg
     3241 cgtcgctggc gctgctcgcc ggatgtgcga gtgtcacgcc gatccgtcaa ctcctcgacg
     3301 atccggcgcg ctacgacggg aaatccgtcc ggatcgaggg tgtggtgaag ggatcggctg
     3361 gggcgt
//
LOCUS       DASUCJ010000029         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1421673, whole genome shotgun sequence.
ACCESSION   DASUCJ010000029 DASUCJ010000000
VERSION     DASUCJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421673"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFU90_00315"
     CDS             <1..454
                     /locus_tag="VFU90_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478237.1"
                     /translation="GRINFADTRVNPSTGTYEMRATLPNADGALKPGQFVRVVLQGAR
                     RVDAIAVPQVAVQDGPQGKFVYVAGKDKDGKDVAMPKPVKVGDWVDASGSHRWIIESG
                     LTAGDPVIVDGIARIMGGGPIVIGAPPAPAQAGTPGKPSGAAAPAAGK"
     gene            458..>1014
                     /locus_tag="VFU90_00320"
     CDS             458..>1014
                     /locus_tag="VFU90_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5478238.1"
                     /translation="MFSRFFIDRPIFAAVIAVFILIAGLASMRSLPIAQYPEIAPPVV
                     TVTAVYPGASAQTIEQTVASPIENAINGVPGMIYMGSQSASDGTLQIQVTFDIGTNVD
                     TAAVNVNNRVKQVEPRLPEEVRRQGVSVERGSSSFLQVLAFYSPDGRYDDLFTSNYVN
                     LNVLDALKRLPGTTNVQIFGAHDYA"
BASE COUNT          170 a          354 c          338 g          152 t
ORIGIN      
        1 ggggcgcatc aacttcgccg acacgcgcgt caatccgtcg accggaacct acgagatgcg
       61 ggcgacgctt cccaacgccg acggcgcgct caagcccggc cagttcgtcc gcgtcgtgct
      121 gcagggcgcc cgtcgcgtcg acgccatcgc ggtcccgcag gttgcggtgc aggacggccc
      181 gcagggcaag ttcgtctacg tcgccggcaa ggacaaggac ggcaaggacg tcgcgatgcc
      241 gaaaccggtg aaggtcggcg actgggtcga tgcgagcggc agccatcgct ggatcatcga
      301 atcgggactc acggcgggcg acccggtcat cgtcgacggc atcgcgagga tcatgggggg
      361 cgggccgatc gtcatcggcg ctccgcctgc gcccgcgcag gccggcaccc ccggaaagcc
      421 atccggcgca gccgcgccgg ctgcggggaa atagcccatg ttctcgcgct tcttcatcga
      481 ccgcccgatc ttcgcggcgg tgatcgccgt gttcatcctg atcgccgggc tcgcgtcgat
      541 gcgctcgctg ccgatcgcgc agtatccgga gatcgcacca ccggtcgtca ccgtgacggc
      601 ggtctacccg ggcgcatcgg cgcagacgat cgagcagacg gttgcgtccc cgatcgagaa
      661 tgcgatcaac ggcgtgcccg ggatgatcta catgggctcg cagtcggcct ccgacggcac
      721 gctgcagatc caggtgacgt tcgacatcgg caccaacgtc gacaccgccg cggtcaacgt
      781 caacaaccgg gtaaagcagg tcgagccgcg gctgcccgag gaagtgcgcc ggcagggcgt
      841 gtccgtcgag cgcggcagct cgtcgttcct gcaggtgctc gcgttctatt cgcccgacgg
      901 ccgctacgac gacctcttca cgtcgaacta cgtcaacctg aacgtcctcg acgcgctgaa
      961 gcgcctgccc ggcacgacga acgtgcagat cttcggcgcg cacgactacg cgat
//
LOCUS       DASUCJ010000030         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_565833, whole genome shotgun sequence.
ACCESSION   DASUCJ010000030 DASUCJ010000000
VERSION     DASUCJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565833"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFU90_00325"
     CDS             <1..368
                     /locus_tag="VFU90_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5478239.1"
                     /translation="MWVAVVSASKNTHAVLRAAGIEDLADAVVDGNIAEQLALPGKPS
                     PATYLEAARRVQATPARAAVVEDAVVGVEAGRAGAFGLVIGVDRGAGAAALLGAGASA
                     VVQDLADVAVTDAHGTPIA"
     gene            760..909
                     /locus_tag="VFU90_00330"
     CDS             760..909
                     /locus_tag="VFU90_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478240.1"
                     /translation="MDSKIRYTKTRDAVSVAYFAGFIFGDRGEFVPKGFDEGVRLWDL
                     RWRET"
     gene            909..>1418
                     /locus_tag="VFU90_00335"
     CDS             909..>1418
                     /locus_tag="VFU90_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5478241.1"
                     /translation="MDQPQIRYTKTGDGVSIAYFAIGSGRTLVYMPPMPTTHVELEWQ
                     VEDLRRGAEGAARAGRFVRYDGRGFALSDRNARDFSVDAMVRDLEAVVDAVGLESFAL
                     MAFQHMAMPALAYASRHPERVRALILPLGYARWEPTGAGPDRIHTTLGLARTDWGMCK
                     EFFAQSIGFD"
BASE COUNT          245 a          460 c          496 g          217 t
ORIGIN      
        1 gcatgtgggt cgccgtcgtg tcggcgagca agaacaccca cgccgtgctg cgcgcggcgg
       61 gtatcgaaga tctcgccgac gccgtcgtgg acggcaacat cgccgagcaa ctcgcgctcc
      121 cggggaagcc ttcgccggcg acgtacctcg aggcggcacg acgcgtgcag gcgacgccgg
      181 cccgggcggc agtcgtcgag gacgccgtcg tcggcgtcga ggcggggcgc gcgggcgcgt
      241 tcggcctcgt catcggcgtc gaccgcggcg ccggcgccgc tgcgctcctc ggcgccggcg
      301 ccagcgcggt cgtgcaggac ctcgccgacg tggcggtgac cgacgcgcac ggcacaccaa
      361 tcgcctgagc agcacgcgct cgtcgtgcgg cggtgaatat cgaagtcaag ggacgctccc
      421 gcgacttcgg aacgatgtat aatatgagtg gaaaggaggt atgaggagcg tacagggtag
      481 acgcagaaca gaccggcgcc ctgacccgta actcgctgta aagcaaacgc tcgaaggcgc
      541 ggattgcggg cggaggtccc tccggagacg gatgcggcga cgccctctcg gttcggaatg
      601 aaccgctagc gagccaagac gaagggcgcc gggacaacag aagaagatgg atgcggcccc
      661 tcgggatcag cccgagggta ttttgttgcc tcgccgcctg tccgtagccg cagagaagcc
      721 gctcgtactt cagcccgctg atcgcggata attccgggca tggattcaaa gatccgttac
      781 acgaagaccc gcgatgccgt cagcgtcgcc tacttcgcgg gcttcatctt cggcgaccgc
      841 ggcgagttcg tgcccaaggg cttcgacgaa ggcgtccgcc tctgggacct gcgctggcgg
      901 gagacgtgat ggaccagccc cagatccgtt acacaaagac cggcgacggc gtgagcatcg
      961 cctacttcgc catcggcagc ggccggacgc tggtgtacat gccgccgatg ccaacaaccc
     1021 atgtcgagct cgagtggcaa gtcgaggacc tgcgacgggg cgcggagggc gcggcgcgcg
     1081 caggaaggtt cgtgcgctat gacggccgcg gcttcgcgtt gtccgatcgc aacgcgcgcg
     1141 acttctccgt cgacgcgatg gtgcgcgacc tggaggcggt cgtcgacgcg gtcggactgg
     1201 agtcgttcgc cctcatggcg ttccagcaca tggcgatgcc ggcgctcgcg tacgcctccc
     1261 gacaccccga gcgagtgcgc gcgctgatcc tgccattggg ctatgcccgt tgggaaccca
     1321 ccggtgccgg accagaccgt attcacacta ccttgggact cgcgcgcacc gactggggaa
     1381 tgtgcaagga gttcttcgcg caatcgatcg ggtttgac
//
LOCUS       DASUCJ010000031         1491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1482866, whole genome shotgun sequence.
ACCESSION   DASUCJ010000031 DASUCJ010000000
VERSION     DASUCJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..722)
                     /locus_tag="VFU90_00340"
     CDS             complement(261..722)
                     /locus_tag="VFU90_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478242.1"
                     /translation="MTPIGANCVAGFDHVPEVVVLMLVVVRFVDLAGVMFFAVLVVRS
                     FNVSFMLVMLFALVRFGGAALADCLARQNFGRYRRGSLGRAVAVRIAVAMAVIVILKI
                     FENVADVEEGVAVESDIDEGGLHAGEDAGDAAFVDATDQRELFFALDINFD"
     gene            712..>1491
                     /locus_tag="VFU90_00345"
     CDS             712..>1491
                     /locus_tag="VFU90_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478243.1"
                     /translation="MGVIPMAISSRTTNSKSASPNRTPRRRKISRRTTIRRRSIRNAT
                     IVARETIAVAKTSAVAKATGAVAIEAETGVDAVASDVAVDVVAVDVQDREARLAQADA
                     ISRHRNMLRPRAISVATIRAATIHAATMIAATDPIKADAATTIAATVVIGAVQDGSTT
                     AAPKADRVLHRRKDRAAKKKFCCRASRSRSIARVPQPPLRQRLSSSTSRTTSRNQSSM
                     NRSRAGLGLNPPTPLVVHPAFQAGCSPARAPKRRPLPRLPKA"
BASE COUNT          307 a          485 c          462 g          237 t
ORIGIN      
        1 agcaggctat tcgtccgcgt cgccgtcgag cacaagctca gtgcgatgcc gtccggtggc
       61 tgcagcccag ttgcagccgc tcccagcgat atcttcggcg catccgcaaa tttttgcggg
      121 ttcattgcgt ccggcgaatc gcggaaaggc ggctccggaa tctccggcgc cgcatatcaa
      181 gaggactttt catgtcgaaa gagttggtta tttccgcagc ctcccacgaa cggcgggttg
      241 cgattttgga agaaggtcag ttagtcgaaa tttatatcga gcgcgaaaaa gagttcgcgc
      301 tggtcggtag catctacaaa ggccgcgtca cccgcgtcct ccccggcatg cagtccgcct
      361 tcgtcgatat cggactcgac ggcgacgcct tcctctacgt cagcgacgtt ttcgaaaatc
      421 ttgaggatta cgatcacggc catggccacg gcgattcgca cagccacggc ccggcccaag
      481 cttccgcgcc tgtaacggcc gaagttttgc ctggcgagac aatcggccag ggccgcgcct
      541 ccgaatcgca cgagggcgaa gagcatcacg agcatgaagc tcacgttgaa gctcctcacg
      601 acgagcacgg cgaagaacat cacgccggcg agatccacga agcgcaccac gacgagcatg
      661 agcacgacaa cttcgggaac gtggtcgaac ccggcaacgc aattcgcacc gatgggcgtc
      721 atcccgatgg cgatctcgag ccgcacaacg aattcgaaga gcgccagccc gaatcgaacg
      781 cctcggcgcc gcaaaatttc gcgccgcact acaatccgac gcagaagtat ccgcaacgca
      841 acgatcgtgg cccgcgagac aatcgcggtg gccaaaactt cggccgtagc gaaggcgacc
      901 ggggcggtgg cgatcgaggc ggagaccggg gtggacgcgg tcgcttcgga cgtcgcggtg
      961 gacgtcgtcg cggtggacgt tcaggaccgg gaggcccggc tggcccaggc ggacgcaatc
     1021 tcccgccatc gaaatatgct tcgccccagg gcgatcagcg tggcaacgat tcgcgcggca
     1081 acgattcacg cggcaactat gatcgcggca accgacccaa tcaaggcgga cgcggctacg
     1141 acaatcgcgg cgaccgtggt gatcggggcg gtccaggacg gttcgacaac cgccgctccg
     1201 aaggcggacc gcgtcctcca tcgtcgcaag gatcgagcag cgaagaagaa attctgctgc
     1261 cgggcgagtc gctcgcgaag tatcgcgcgc gtcccgcagc cgccgctccg tcagcgcctg
     1321 tcgtcgagca cgagtcgcac gacgagccgc aaccaatcat cgatgaaccg atctcgcgcg
     1381 ggtctgggtc tcaacccacc aacgcccctc gtcgttcatc cggccttcca agctggttgc
     1441 tcgccggctc gggcgccgaa gcggaggccg ctgccgaggc tgccgaaggc t
//
LOCUS       DASUCJ010000032         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_703414, whole genome shotgun sequence.
ACCESSION   DASUCJ010000032 DASUCJ010000000
VERSION     DASUCJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703414"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFU90_00350"
     CDS             <1..683
                     /locus_tag="VFU90_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478244.1"
                     /translation="RAGFRYVFLGIENVLDEDLAFLKAKAKNAQREDGRTVGSATLTA
                     VDLLHRHGMLVVGGLIVGSPDDTKASIEANVEFAKKYVDWPYIQHPTPYPGTPMTKDF
                     VERNLIVNDAVEEYDGTTAVVRTEHLSAEDVEFMRWRAERWMKVRHMPDALSHDTWFV
                     LTHWHKMLRHTFRGTTLRTWLGLESTRDAFRRYKSIRRREREFFPPAGPRTGHDTTAS
                     TSLAHGRV"
     gene            680..>1190
                     /locus_tag="VFU90_00355"
     CDS             680..>1190
                     /locus_tag="VFU90_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478245.1"
                     /translation="MSAAPGPPAANSGRIVTDYEAYLFGEGHWLRAWEKMGGRPAELD
                     GVRGYSFVLWAPNARRVSIVGDFNGWDGRAHPMRNLGASGLWEGFVPGLGEGVLYKFE
                     LQTQSGPPFTRADPFALRAEIPPQTASITGTLAGYVWRDDAWMEARRAAREARLDLPM
                     AIYEVHPGSW"
BASE COUNT          198 a          391 c          408 g          193 t
ORIGIN      
        1 gccgcgccgg cttccgctac gtgtttctcg gcatcgagaa cgtgctcgac gaagacctcg
       61 cgtttctcaa ggcgaaagcg aaaaacgccc agcgcgagga tggccgcacg gtcggcagcg
      121 cgacgctcac cgccgtggac cttctccacc gccacggcat gctcgtcgtc ggcgggttga
      181 tcgtcgggag tccggacgac acgaaggcgt cgatcgaagc gaatgtcgag ttcgcgaaaa
      241 aatacgtcga ctggccgtac atccagcacc cgacgccgta tcccggcacg ccgatgacga
      301 aagattttgt cgagcggaat ctgatcgtca acgatgccgt cgaggaatat gacgggacga
      361 cggcggtggt gcggacggag catttgagcg cggaagacgt cgagttcatg cggtggcggg
      421 cggagcgctg gatgaaagtg cgccacatgc ccgacgcctt gtcgcacgat acgtggttcg
      481 tgctcacgca ctggcacaag atgctgcggc acacgttccg cggcaccacg cttcgcacgt
      541 ggctgggtct agagtccacg cgcgatgcgt tccgccgcta caagtccatt cgccgccgcg
      601 agcgcgagtt ctttccgcca gcgggcccgc gcaccggtca cgacaccacc gcttccacca
      661 gcctggcgca cggacgcgtg tgagcgccgc ccccggtccg cccgccgcca actccggacg
      721 catcgttacc gactacgagg cgtatctctt cggcgagggc cactggctcc gcgcgtggga
      781 gaagatgggc gggcgaccgg cggaactcga cggcgtgcgt gggtactcgt tcgtgctctg
      841 ggcgccgaac gcgcggcgcg tctcgatcgt cggcgacttc aacggctggg acggacgcgc
      901 gcacccgatg cgcaacctcg gggccagcgg attgtgggaa ggcttcgtgc ccggccttgg
      961 cgagggcgtg ctctacaagt tcgagctgca aacgcagagc ggtccgccgt tcacgcgcgc
     1021 cgatccgttt gcgctgcgcg cggagattcc gccgcagacg gcgtcgatta ccggcacgct
     1081 tgccggctac gtctggcgtg atgacgcgtg gatggaggcc cgtcgcgccg cgcgcgaggc
     1141 ccggctcgac ttgccgatgg cgatctacga ggtccatccg ggatcgtggc
//
LOCUS       DASUCJ010000033         1242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_596455, whole genome shotgun sequence.
ACCESSION   DASUCJ010000033 DASUCJ010000000
VERSION     DASUCJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596455"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFU90_00360"
     CDS             complement(<1..746)
                     /locus_tag="VFU90_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5478246.1"
                     /translation="MAVPVEGLAAAVRGRVVERGASDYDDARALYNAMIDKRPAAIAY
                     CVDESDVAAAVWYANEHGLRIAIRSGGHNGGGHGSVDDGLVIDLSQMKKITVDAAARV
                     ARVQGGALLKEALETLHQHGLTIPVGVIGTTGVGGLTLGGGIGHFTRSFGLTIDNLLG
                     ATVVLADGSIVECDADREPDLFWAIRGGGGNFGVVTAFTFRLHPVSTVIAGPTFWPID
                     QAAEVLRTYREFLPAADRDLNGFFLFTSVPP"
     gene            complement(835..>1242)
                     /locus_tag="VFU90_00365"
     CDS             complement(835..>1242)
                     /locus_tag="VFU90_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5478247.1"
                     /translation="GELGQGKGAFYKPTVLSNVDNAMEVAQEEIFGPVVTIIPFEDEK
                     DAIRIANDVKYGLFATVWTGDPARGHRVARAIKSGMVGINTPYTAFPGIPFGGYKQSG
                     FGRELSIETLDLYLETKGVLVSTSPKPFNPFGL"
BASE COUNT          194 a          443 c          398 g          207 t
ORIGIN      
        1 ggcgggacgg acgtgaacag gaagaacccg ttcaggtcgc gatcggccgc aggcaggaac
       61 tcgcggtacg tccgcagcac ctcggccgcc tggtcgatcg gccagaacgt cggcccggcg
      121 atgacggtgc tcaccggatg cagtcggaac gtgaacgccg tgacgacgcc gaagttgccg
      181 ccgccgccgc ggatcgccca gaacagatcg ggctcgcggt ccgcgtcgca ttcgacgatc
      241 gatccgtccg cgagcacgac cgtcgcgccg agcaggttgt cgatcgtcag gccgaacgat
      301 cgcgtgaagt gaccgatccc gccgccgagc gtcagcccgc cgacgccggt cgtgccgatc
      361 acccccaccg gaatcgtcag cccgtgctgg tgcagcgtct cgagagcctc tttcagcagt
      421 gcgccgccct ggacgcgcgc gacccgcgcc gcggcgtcca cggtgatctt cttcatctgc
      481 gacaggtcga tcacgagccc gtcgtcgacg ctgccgtggc cgccaccgtt gtgaccgccg
      541 ctgcggatcg cgatccgcag accgtgctcg ttcgcatacc aaaccgcggc cgcgacgtcc
      601 gactcgtcga cgcagtacgc gatcgccgcc ggtcgcttgt cgatcatcgc gttgtacagc
      661 gcgcgcgcgt cgtcgtagtc cgaagcaccg cgctcgacga ctcgcccgcg gactgctgcg
      721 gcgagccctt cgactggaac agccatctcc gcctccgatg cgattcgtct tccagccatt
      781 cacgctacgg agggccggcc ggcctgcgga tcggtgggat tgcctatctt gcggctacag
      841 gccgaacggg ttgaacggct tggggctcgt cgagacgaga acgcccttcg tctcgagata
      901 gaggtcgagc gtctcgatcg acagctcgcg gccgaagccc gactgcttgt acccgccgaa
      961 gggaatgccc gggaatgcgg tgtacggcgt gttgatgccg accatgcccg acttgatcgc
     1021 gcgcgcgacg cgatgcccgc gtgcgggatc gcccgtccac accgtcgcga acagcccgta
     1081 cttcacgtcg ttcgcgatcc ggatcgcgtc cttctcgtcc tcgaacggga tgatcgtgac
     1141 gaccggtccg aagatctcct cctgtgcgac ctccatcgcg ttgtcgacgt tcgagagcac
     1201 cgtcggcttg tagaacgcgc ccttcccctg gccgagctcg cc
//
LOCUS       DASUCJ010000034         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1253619, whole genome shotgun sequence.
ACCESSION   DASUCJ010000034 DASUCJ010000000
VERSION     DASUCJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253619"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFU90_00370"
     CDS             complement(<1..511)
                     /locus_tag="VFU90_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5478248.1"
                     /translation="MPSATLDPRTALVVIDLQKGIVGMPTAHPSPAVVRNAAGLAAAF
                     RKAGLPVVLVNVAFAPDGADRFQPRADAAPAARSFAADFAEIVPELGAQPDDIRITKR
                     HWGAFYGTELDLQLRRRGVTGIVLAGISTSIGVESTARSAFEHGYNLTFASDAMTDSK
                     DASHRHSLEH"
     gene            complement(552..1007)
                     /locus_tag="VFU90_00375"
     CDS             complement(552..1007)
                     /locus_tag="VFU90_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5478249.1"
                     /translation="MHVALILAGVVFFPFASAQTGNDCCATLRGTVTTGYPAHQPIPN
                     VRVIAISDSDVRETRTDAHGNYLFLTLLPGVYRVSTYPEKVDPTRPHPEMIVFSTWAG
                     SVVPRPGMPAYVGTQGENFRRCAEEAQAPVELSAGLEYVANIDLVTRCP"
     gene            1089..2438
                     /locus_tag="VFU90_00380"
     CDS             1089..2438
                     /locus_tag="VFU90_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEU5478250.1"
                     /translation="MRRLAGIFVRVFEYAMPDPYIFAVALTLLTGALALAFAPHRGIA
                     EVLSGWYEGIFDIFAFALQMILVLVTGYALAASAPVRRALGALAKLPRTPKSAVALTF
                     VASAVACWLNWGFGLVVAGLLARTIASRMRIDFGWLVAAAYAGFVVWASGLSSSIALA
                     QATPGSKLNIVQALTGVVLPLRETIFTPFNLVPTIVLLVLLPFVFMALEPRGDDVVAG
                     DVTKLATEDAERGVVPAPAKSGTLAAALERAWILNLVLAVAGIAYLAITWATRGFSLD
                     INAVIFIFLIAGLLMHWRPIAYVNAINGAARITGPLLIQYPLYGGIMGIMTATGLAGV
                     IASWFLTFSTRATLPFWSYAASIVLSLFVPSGGGHWAVQGPFVVPAAARLGASQSATA
                     MAVAIGEEVANMIQPFWALPVLAIAGVALQRVMAFTVVVFFIAGIVFGASLLLLVPH"
BASE COUNT          393 a          779 c          792 g          482 t
ORIGIN      
        1 tgtgctcgag gctgtgccga tgggaagcgt ccttcgaatc ggtcatcgca tcgcttgcga
       61 acgtcaggtt gtagccgtgt tcgaaggccg atcgcgcggt cgattcgacg ccgatgctcg
      121 tcgagatgcc ggcaagcacg atacctgtga cgccgcgccg gcgaagctgc aggtcaagct
      181 cggtgccgta gaacgcaccc cagtggcgtt tggtgatccg gatgtcgtcg ggctgcgcgc
      241 cgagctccgg cacgatctcg gcaaaatccg ccgcgaagga acgcgccgcg ggcgccgcat
      301 ccgctcgtgg ctggaagcgg tcggcaccgt cgggagcgaa ggcgacgttg accaacacga
      361 cgggcaggcc ggctttgcgg aatgccgcag ccaagcccgc cgcattccgt actaccgccg
      421 gcgacggatg cgcagtcggc atgccgacga tgcccttctg caagtcgatg acgaccagcg
      481 ccgtgcgcgg atctaaggta gcggaaggca tagccagccc tgacggttcg gcgccgggcg
      541 gcgccctccg ctcacggaca gcgagtgact aagtcgatgt tcgcgacgta ttcgagaccg
      601 gccgagagtt cgaccggggc ttgcgcttcc tcggcgcacc gccggaagtt ctctccctgc
      661 gtcccgacgt acgccggcat tcccggccga ggcacgacgc tgccggccca cgtcgagaaa
      721 acgatcatct cgggatgcgg gcgtgtcgga tcgacctttt cgggataggt cgagacgcga
      781 tagacgcccg gaagcagcgt cagaaagagg tagttgccgt gcgcgtcggt ccgagtctcc
      841 cgcacgtcgc tgtcggaaat agcgatcacc cgtacgttcg ggattggctg atgcgcgggg
      901 tagccggtcg tgaccgtgcc gcgcagcgtc gcgcagcagt cgtttccggt ttgcgcgctc
      961 gcaaacggaa agaatacgac accggcgagg atcaaggcga cgtgcatatc caactagcgt
     1021 tcggacgaag ctccgcgtga cgcacccttc gtaggccaaa cggacccgag ccgtcgaaac
     1081 ggcaccccat gcgacggctg gccggaatat tcgtccgggt attcgagtac gccatgcccg
     1141 acccgtacat ttttgcggtc gcgctaaccc tgcttaccgg agcgttggca ttagccttcg
     1201 ctccgcatcg cgggatcgcc gaggtgctga gtggttggta cgagggcatc ttcgatatct
     1261 ttgcctttgc cttgcaaatg attctcgtgt tggtgacggg ctacgcgctg gcggcgagcg
     1321 cgccggtaag gcgcgcgctc ggcgcgctgg ccaaactccc gcgcacgccc aagtcggccg
     1381 tcgcgctcac cttcgtcgct tcggccgtcg catgctggct gaactggggc ttcggactcg
     1441 tggtcgcagg gctgctggcg cgtacgattg cgagtcgcat gcggatcgat ttcggttggt
     1501 tggtggccgc cgcatacgcc ggcttcgtcg tttgggcaag cgggctttcc agctcgatcg
     1561 cactcgctca agctacgccc ggcagcaaac tcaacatcgt tcaggccctc accggggtcg
     1621 ttcttccgtt gcgcgaaacg attttcacgc cctttaatct cgtccccacg atcgtgctgc
     1681 tggtgcttct gcccttcgtc ttcatggcgc tcgaaccgcg tggcgacgat gtcgtcgccg
     1741 gcgacgttac gaaactcgct accgaagatg cggaaagggg cgtcgttcct gcacccgcga
     1801 aatcgggaac gcttgccgcc gcgttggagc gcgcctggat tctaaacctg gtcctcgccg
     1861 tggccgggat agcctacctc gccataacgt gggcgacccg cggattctcg ctcgacatca
     1921 acgcggtcat cttcattttc ctcatcgccg gcctgctgat gcattggcgg ccgatcgcct
     1981 acgtcaacgc gatcaatggt gcggcacgaa ttaccggacc gctcttgatc cagtatccgc
     2041 tctacggcgg catcatgggg atcatgacgg cgacgggttt ggccggagtc atcgcgagtt
     2101 ggtttctcac tttttcgacg cgcgctacct tgccgttttg gagctacgcc gcgagcatcg
     2161 tactatcgct gttcgtgccg agcggcggcg ggcactgggc ggtgcagggg ccgttcgtcg
     2221 tcccggctgc agccaggctc ggcgcgtcgc aaagcgcaac cgccatggcg gtcgcaatcg
     2281 gcgaagaagt cgccaacatg attcagccct tctgggcgct ccccgtactc gcaatcgccg
     2341 gggtcgcgct gcagcgggtg atggctttca ccgtggtcgt gttctttatt gccggaatcg
     2401 tttttggagc gagccttctg ctgctcgtcc cgcactagga gcgccg
//
LOCUS       DASUCJ010000035         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_168540, whole genome shotgun sequence.
ACCESSION   DASUCJ010000035 DASUCJ010000000
VERSION     DASUCJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168540"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFU90_00385"
     CDS             complement(<1..401)
                     /locus_tag="VFU90_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478251.1"
                     /translation="MNVWAAFAGGLVGTVVLTTTMRAASELRLTRMDLPFLLGTAIST
                     DRGRAKALGYLAHFAFGFLFALAYDAIFAATGEAGWVFGALLGLLHGLFAGTALVNVL
                     LPTVHPRMGTAFTASDTTPVLEPPGFLLRNYG"
     gene            complement(398..>1025)
                     /locus_tag="VFU90_00390"
     CDS             complement(398..>1025)
                     /locus_tag="VFU90_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5478252.1"
                     /translation="IWRQPDAGLWEVRSGPQHFTQSKMMCWVALDRAVRLAEQGAIPG
                     RQIGQWRLAMKEIVQFVDQHCWSDERNTYTRHAGTDDLDASLLLGVLFAYGDAGDPRT
                     EATVDAIRRELTQGPLVRRYTGDDGLHGTEGAFVACSFWLVEALSLLGHRGEAATLMD
                     ELVSLGNDVGLYAEEIDPVTGAFLGNFPQGLTHLALINAAVALNARDT"
BASE COUNT          188 a          327 c          346 g          164 t
ORIGIN      
        1 ccgtagttgc gcagcaagaa tccgggcggc tcgaggaccg gagtggtgtc gctcgcggtg
       61 aaggcggtcc ccatgcgcgg gtgaacggtc ggaagcagca cattgacgag cgccgtgccc
      121 gcgaacaatc cgtgcagaag gccgaggagc gcgccgaaga cccagcctgc ttcgccggtc
      181 gcggcgaaga tcgcgtcata cgcgagcgcg aacaagaacc cgaacgcgaa gtgagcgaga
      241 tagccgagcg cctttgcgcg tccgcggtcg gtgctgatcg ccgtgccgag aaggaacgga
      301 agatccattc gcgtgagccg cagctcgctg gcagcgcgca tcgttgtggt gagcacgacc
      361 gtgccgacca atcctccggc gaacgcggcc cagacgttca tgtgtccctc gcgttcagcg
      421 cgaccgcggc gttgatgagc gcgagatgcg tcaaaccctg cggaaagttg ccgaggaacg
      481 ctcccgtgac gggatcgatt tcttccgcat agagaccgac gtcgttgccc agggagacga
      541 gttcgtccat gagcgtcgcg gcttcacccc gatgcccgag cagcgagagg gcttccacga
      601 gccagaagga acatgcgacg aacgcgcctt cggtgccgtg caggccgtcg tcgcccgtgt
      661 accggcgcac gagcggtcct tgcgtgagct cccgacgaat cgcgtcaacg gtggcttcgg
      721 tgcgagggtc gccggcatcg ccgtacgcga acaacacgcc gagaagaagg cttgcgtcga
      781 ggtcgtcggt acccgcgtgc cgcgtgtacg tgttgcgctc gtccgaccag cagtgttgat
      841 cgacgaactg gacgatctcc ttcatggcga gtcgccattg accgatttgc cggcccggta
      901 tggccccctg ttcggcaagc cggacggccc ggtcgagcgc gacccagcac atcatcttcg
      961 actgcgtgaa atgttgcgga ccactgcgca cttcccacaa gccggcgtcg ggctgtcgcc
     1021 agatg
//
LOCUS       DASUCJ010000036         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_76819, whole genome shotgun sequence.
ACCESSION   DASUCJ010000036 DASUCJ010000000
VERSION     DASUCJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76819"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFU90_00395"
     CDS             <1..261
                     /locus_tag="VFU90_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478253.1"
                     /translation="VFANRTEPQTRGRERQARTRLLNTVLKYHGIVVKDWSGSSYLLT
                     SLTGRTALVDTIADVWPAAERLAGRTIDPLDEQYLATLAKPN"
     gene            272..>1078
                     /locus_tag="VFU90_00400"
     CDS             272..>1078
                     /locus_tag="VFU90_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5478254.1"
                     /translation="MAEYSELTPVNIITGFLGSGKTTLLQRLLRSPDLRDVAVLVNEF
                     GEVGLDHHLLQGVAESTLLLGNGCVCCAVRGDLQRALRDLLSRRTRGEVPYFRRVVIE
                     TSGLADPAPIAYTLLSEAILRHHFRLSGIVTTVDAVNGASQLDRFPESVKQSSMADRL
                     VMTKADLADEGTMAALRQRLRRLNVSAQILDSREVGGDFSQLLTDDIYDTVGKSREAR
                     HWVAEEVDEHATHHHTADVQSFAVIFDEPLDWTAFGVWASMLLHRHGADIL"
BASE COUNT          185 a          348 c          342 g          203 t
ORIGIN      
        1 gtttttgcga accggaccga gccgcaaacg cgcggtcgcg agcgccaggc gcgcacccgc
       61 cttctcaaca ccgttctgaa gtatcacggc atcgtcgtga aggattggtc gggcagttcg
      121 tacctgctca ccagcctcac cggccgcacc gccctcgtcg atacgatcgc ggatgtctgg
      181 ccggccgccg agcggctcgc cgggcgaacc atcgatccgc tggacgagca atatctcgcg
      241 acgctcgcca aaccaaactg aacgccgaac aatggccgag tacagcgagc ttacgcccgt
      301 caacatcatc accggctttc tcggcagcgg gaagacgacg ctgttgcagc ggctcctgcg
      361 ttcgccggac ctgcgcgacg tcgccgtgct ggtcaacgaa ttcggcgaag tcggactcga
      421 tcatcatctg ctgcagggcg tggccgagag tacgctgtta ctcggcaacg gctgcgtgtg
      481 ctgcgcggtg cgcggcgacc tgcagcgggc gttgcgcgat ctgttgtcgc ggaggacgcg
      541 cggcgaggtg ccgtattttc gccgagtggt gatcgagact tccggcttgg ccgatccggc
      601 gccgatcgcc tacacgctgt tgtcggaagc cattcttcgt catcatttcc ggttgtcggg
      661 gatcgttacg actgtggatg cggtcaatgg cgcgagccag ctcgaccgtt ttccggagtc
      721 ggtcaaacag tcgtcgatgg ccgaccggct ggtgatgacc aaggcggact tggcggacga
      781 ggggacaatg gcggcgctcc gccagcgact tcgccgcctc aacgtctccg cgcaaatcct
      841 cgactcgcgc gaagtcggcg gcgatttctc gcaattgctg acggatgaca tctacgatac
      901 cgtcggcaag tcgcgggagg cgaggcactg ggttgcggaa gaggtcgacg agcacgccac
      961 ccatcaccac accgccgacg tgcaatcctt cgccgtcatt ttcgatgagc cgctcgattg
     1021 gaccgctttc ggcgtctggg ccagcatgct gctccatcgc cacggtgccg atatcctc
//
LOCUS       DASUCJ010000037         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_382489, whole genome shotgun sequence.
ACCESSION   DASUCJ010000037 DASUCJ010000000
VERSION     DASUCJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382489"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFU90_00405"
     CDS             <1..1159
                     /locus_tag="VFU90_00405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5478255.1"
                     /translation="KFDLNASEPYWAPDGKSIYFSTDTRESDEVFAANVADGSVKQMT
                     TMPGVISIDAVSADGHTVVGTRADPTHPADVYRSDLSFATIEPITNQNGWLSDYALGT
                     TEVITWKSSKDGMEIDGIVTKPVDFAASRKYPALLNPHGGPTGASLLGFNPGEQIMAA
                     NGYVVLEPNFRGSTGRGEAFAMANQNDWGGGDYRDDMSGVDALIARGWADPDRLGAFG
                     WSYGGFMTFWIDTQTDRFKAISPGAGLPDLYSMYSQTDIHRYLRQFFNMKAPWDNFQE
                     YWDHSPMKFVNNVKTPTMILHGQADTRVPIPQAEEFFRALTERHVPVEFVTYPRENHG
                     FTEPRHIQDRYQRYLVFFGKYLNNPPVTEPRDVIERMNKDLPAQQVATTKP"
BASE COUNT          239 a          399 c          407 g          214 t
ORIGIN      
        1 caagttcgac ctcaacgcga gcgagcccta ctgggcgccg gacggcaagt cgatctactt
       61 ctcgacggac acgcgcgagt cggacgaggt gttcgccgcg aacgtcgccg acggaagcgt
      121 gaagcagatg acgacgatgc ccggcgtgat cagcatcgat gcggtgagcg ccgatggaca
      181 cacggtggtg gggacgcgcg cggatccgac gcatccggcg gacgtgtacc gctccgacct
      241 gtcgttcgcc acgatcgagc cgatcaccaa ccagaacggg tggctctccg actatgcgtt
      301 aggcacgacc gaggtcatca cgtggaagag cagcaaggac ggcatggaga tcgacggcat
      361 cgtcaccaag ccggtcgact ttgcggcgtc gcgcaagtac cccgcgctgc tcaacccgca
      421 cggcggtccc accggcgcct cgctcctcgg cttcaacccg ggcgagcaga tcatggctgc
      481 gaacggctac gtggtgctcg agccgaactt ccgcggttcg accggtcgcg gtgaagcctt
      541 cgcgatggcg aatcagaacg actggggcgg cggcgactac cgcgatgaca tgagcggcgt
      601 ggacgcgctc atcgcgcgcg gctgggccga ccccgatcgg ttaggcgcgt tcgggtggtc
      661 gtacggcggg ttcatgacgt tctggatcga tacgcagacg gatcgattca aggcgatttc
      721 gccgggcgcc ggtctgcccg atctgtattc gatgtactcg cagacggaca tccatcggta
      781 cttgcgccag ttcttcaaca tgaaggcgcc ctgggacaac ttccaggaat actgggatca
      841 ctcgccgatg aagttcgtga acaacgtgaa gacgcccacg atgatcctgc acggccaggc
      901 cgatacgcgc gtgccgattc cgcaggccga ggagtttttc cgtgcactta ccgagcgcca
      961 tgtccccgtg gagttcgtca cgtacccgcg tgagaaccat ggattcacgg agccgcggca
     1021 catccaggac cgctaccagc ggtatctggt gttcttcggc aagtacctca acaatccgcc
     1081 ggttaccgag ccccgcgacg tgatcgagcg catgaacaag gatctccccg cacaacaggt
     1141 cgcgaccacc aaaccatgat gtcgttacgt cgattgatgg cgacgccgcg cgttcggggc
     1201 gcgttaggca caacggtcct ggccgcgctc ggtgtcggcg tcgcggcgtg ttcgcacgg
//
LOCUS       DASUCJ010000038         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_413070, whole genome shotgun sequence.
ACCESSION   DASUCJ010000038 DASUCJ010000000
VERSION     DASUCJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413070"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFU90_00410"
     CDS             complement(<1..909)
                     /locus_tag="VFU90_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5478256.1"
                     /translation="MTTSAAEGAGSLQPDQEAYARRLSRRRRFFRVIVGITALVHVPV
                     ALAVTALANHLGWPLPGLIGMAWALVGVALFTSRVSIAAADARRNVWTIRLVDIPYFI
                     HWCAAVWALIPSIVAVLVAVITDLARGVPLHAPMDAFMWSYLSGLVVCGYGTLVRRRW
                     FHVVERDVAIRGLDKRLDGLRIAHLSDLHIGSLTPRSWGLRWAGAANGQQPDLTVVTG
                     DLVTSGNVFHDDVADVIGALRAKHGVFVSMGNHDYFGDGEPLVTKMRERGVTVLRNEG
                     TRVEHQGGAVWLAAIDDTWTRRDDIQA"
     gene            complement(984..1307)
                     /gene="trxA"
                     /locus_tag="VFU90_00415"
     CDS             complement(984..1307)
                     /gene="trxA"
                     /locus_tag="VFU90_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5478257.1"
                     /translation="MAGANVLEINDLNFEAEVLKSHVPFLLDFSAVWCGPCRVLAPVV
                     EKLADEYEGKARVGKLDIDDSPAVASKFGIRGVPTVLVFKDGKESGRHVGVTSKEVLV
                     KMLGV"
BASE COUNT          290 a          576 c          508 g          248 t
ORIGIN      
        1 cgcctggatg tcatcgcgcc gcgtccacgt atcgtcgatc gcggcaagcc acacggctcc
       61 gccttggtgc tcgacgcgtg ttccctcgtt gcgcagcacg gtgacgccac gctcgcgcat
      121 cttggtgacg agcggctctc cgtcgccgaa gtagtcgtga ttgcccatcg acacgaagac
      181 gccgtgtttc gcgcgaaggg cgccgatgac gtccgcgacg tcatcgtgga agacattgcc
      241 gctcgtgacg aggtctcccg tcacgaccgt caaatcgggc tgctgcccgt ttgccgcgcc
      301 cgcccagcgg agcccccagg atcgcggcgt gagggacccg atatgcagat ccgacaggtg
      361 cgcgatccgc agtccgtcca gtcgcttgtc gagcccccga atggcgacgt cacgctcgac
      421 gacgtgaaac caacgacgcc ggacgagcgt cccgtacccg cagacgacga ggcccgacag
      481 gtagctccac atgaacgcat ccatgggcgc gtgcagcgga acgccgcgag cgaggtccgt
      541 gatgacggcc acgagaaccg ccacgatcga cgggatcagc gcccagacgg ccgcgcacca
      601 gtggatgaag taggggatgt cgacgagccg gatcgtccac acgttgcggc gtgcgtccgc
      661 cgcggcgatg ctcacccggc tcgtgaagag ggccacgccg accagcgccc acgccatgcc
      721 gatcagaccc ggcagcggcc acccgaggtg attcgccagc gccgtcaccg cgagcgcgac
      781 cgggacgtgc acgagcgccg tgatcccgac tatgacccgg aagaagcggc gccggcggct
      841 cagccggcgc gcatacgctt cttggtctgg ttggagcgag cccgcccctt ccgcggccga
      901 cgtcgtcatg cggcgccgat cacgcggggc gcgaccccgg ccgatgaatc catctcagag
      961 agaacgcgac tctcggcgtt cgctcagacg cccagcatct tgaccagcac ttccttgctg
     1021 gtcacgccga cgtgccgccc ggactccttg ccgtccttga agacgagaac cgtcggcacg
     1081 ccacggatgc cgaacttcga cgccaccgcc ggcgaatcgt cgatgtcgag ctttccgaca
     1141 cgggctttgc cctcatactc gtccgccagc ttctcgacca cgggcgccag cacacggcac
     1201 gggccgcacc agactgcgct gaaatcgagg aggaagggaa catgcgactt gagcacttcg
     1261 gcttcgaagt tcaggtcatt gatttcgagg acattcgcgc cagccatact tcactccagg
     1321 tttcgctctc gcgttcgaac cgcgaggatc tctcttataa ggctccggtg cggccctgtc
     1381 gatgggcgta gcggcgcgag gacgcgccct gaacttggcc ccgacgcgtc cgcccatgca
     1441 tggtcgcccg ttcggagggc cgacgatgaa gaacgaacaa tcgaccgcga tgaacgagcc
     1501 gagccgcccg gagcggcgac gtcaccgcgt ctacgtcacc cacaataccg agtaccacat
     1561 ccgcgaccgc cgctgcgtcg ccgtgcgcga tcggcgcacc ggtgagtttc tccaaggcca
     1621 tc
//
LOCUS       DASUCJ010000039         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_642320, whole genome shotgun sequence.
ACCESSION   DASUCJ010000039 DASUCJ010000000
VERSION     DASUCJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642320"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFU90_00420"
     CDS             <1..1130
                     /locus_tag="VFU90_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5478258.1"
                     /translation="ASILTRSLLKTESLPLGFDRSHLIVADLDIATPGYVSDRLANAA
                     HTLREHLLSVPGVTNVSYSQNGIFSGSEWHTSVGIPGLTLTMRDSVTAADRAGSGYAR
                     TIGAHLITGRDFGPEDEGVPPATALVNESFARFYFHGANPIGQLVQFDDSSVVHIVGE
                     IADVRGQSLDTTGRSGAARRIYIPYLSRSGTTKFPQPAELRLLVRTSGDPAQVLQAVR
                     RAIVATDPALTIDDLESVTQLIRFSIRDQRLVARLASGLGALAVVLAAIGLFGVMSYS
                     VGRRTTEIGVRSALGAERSTIALMVLRDGLRPVALGVLLGMPLSIAAVRLLQHHLNDI
                     SSDPASIAVAIGVLLTAAAAAVLVPARRATRIDPIGALREE"
BASE COUNT          166 a          380 c          375 g          214 t
ORIGIN      
        1 gcgcgtcgat cctcacgcgc agcctgctga aaaccgaatc gctgccgctc ggcttcgatc
       61 gctcgcatct catcgtcgcc gatctcgaca ttgcgactcc gggatatgta tcggaccgcc
      121 tcgcgaacgc cgcgcatacg ctccgcgagc atctcttatc ggttccgggt gtgaccaatg
      181 tgtcgtactc gcagaacggc atcttttcag gcagcgagtg gcacaccagt gtgggcatcc
      241 ccggcctaac gctcaccatg cgcgacagcg tcaccgccgc ggacagggcc gggtcgggat
      301 atgcccgtac tatcggggcg catctgatca ccggccggga tttcggcccg gaagacgagg
      361 gcgtgccgcc ggcaacggcg ctcgtcaacg agtcgttcgc gcgattctat tttcacggcg
      421 cgaatccgat tgggcagctc gtgcagttcg acgactcgag cgtcgtccac atcgtgggcg
      481 agatcgcgga cgtccgcgga cagtcgctcg acacgacggg caggtccggc gccgcgcgcc
      541 gcatctatat cccgtatctc tctcgaagcg gcacgacaaa gtttccccag ccggccgagc
      601 tgcggctctt ggtgcggacg agcggcgatc ccgcacaggt gctgcaggcg gttcggcgcg
      661 cgatcgtggc caccgatccg gctctcacga tcgacgacct cgagtcggtc acccagctca
      721 tccgcttctc catccgggat cagcgcctcg tcgcgcggct ggcgtccggt ctcggcgcgc
      781 tcgcggtggt gctggcggcg atcggcttgt tcggcgtgat gagctacagc gttggccgcc
      841 gtaccaccga aatcggcgtt cgttcggcgt taggcgccga acggtcgacg atcgcgttga
      901 tggtgctccg tgacggcctg cgtccggtcg cgctcggcgt cctgctcgga atgccgttgt
      961 ccattgccgc cgtgcgcctg ctgcagcatc atctcaacga tatctcgagc gacccggcgt
     1021 ccatcgcggt tgcgatcggg gtgttgctga cggcggccgc agcggctgtg ctcgttccgg
     1081 cgcggcgggc gacgaggatc gatccgattg gagcgctccg cgaggagtga cgtag
//
LOCUS       DASUCJ010000040         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1207869, whole genome shotgun sequence.
ACCESSION   DASUCJ010000040 DASUCJ010000000
VERSION     DASUCJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207869"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFU90_00425"
     CDS             complement(<1..804)
                     /locus_tag="VFU90_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478259.1"
                     /translation="MLRFITALVAVFLVYLAGASAAWAATDGLVRGTVTVDGKPAAGA
                     HVTLEGEGSRFSTTAAAGGTYGFPQVPFGQYRLAAEVKGLPPLQTFVTVSSNTVATVD
                     LPLTTHLKQIAQTTVTARAGLQANPPSVNQLSRTNIQASPQQNSLDKLLATLPGVIPF
                     SFNEPVINGFHGVTYNIDGAPLPLATTSNFAEIVDPKNIDSVELLTGAIPAEYGGDRM
                     GGVVNIITNRPADLPEGLYGAMTGGFGNQSQGIGSLETAARFGASELFVN"
     gene            complement(856..1407)
                     /locus_tag="VFU90_00430"
     CDS             complement(856..1407)
                     /locus_tag="VFU90_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478260.1"
                     /translation="MTAREIPGALALGLLASLLAHIALYGGGHAMGGAYHELLLDLAA
                     GGTVAFAAAVAALTWNGARRTADGSILAARMTACIPSWGGLVAGTAAWFALGERFDSP
                     HSAAGLLVTLVALGAAAWVVRRVAGAAVRLLARAILRLARLTFAPRLPVRAAAIRIAP
                     APYRTPLLRRRFARPPPIAGARA"
     gene            1535..2086
                     /locus_tag="VFU90_00435"
     CDS             1535..2086
                     /locus_tag="VFU90_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478261.1"
                     /translation="MSLRTRVENADTVSLVCALLVRFPELASIRSMPVDRTVRLTFAL
                     RQRLDRHAQAALTEALDDHIRSFLALFGDDPETLRFECESDRSMTFVHITRDLESFTK
                     EELELQVAFFGERFSDALVRNPVPDDHVDADPAAQDEAALFAVEALRDPAQSKSLVGF
                     REEKRVLVYFLKSQKKAKASARR"
     gene            2104..2877
                     /locus_tag="VFU90_00440"
     CDS             2104..2877
                     /locus_tag="VFU90_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEU5478262.1"
                     /translation="MVGDVVGSPGRDTLKRCVALAREQHHIHACLANGENAAAGFGLT
                     AQTAEEMFESGVDFITSGNHIFDKRDFREYLETSDRVIRPANYPPTAPGRGSGTLAVD
                     GVKIGILNLMGRTFMPPVDDPFRCADALLEDLQRDARVIVVDMHAEATSEKVAMGRYL
                     DGRVSCVFGTHTHVQTSDEQILPGGTAYITDVGMTGPTDGVIGMESRTVLDRFLNGVS
                     ERFNVQKTGSKQFCAAVVAIDADSGKAIEIKRIYTRGIA"
     gene            2888..3154
                     /locus_tag="VFU90_00445"
                     /pseudo
     CDS             2888..3154
                     /locus_tag="VFU90_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            3250..4062
                     /locus_tag="VFU90_00450"
     CDS             3250..4062
                     /locus_tag="VFU90_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5478263.1"
                     /translation="MIIDFHSHSNMSDGTLTPQALADFMGERKVEMFCISDHDTLSAY
                     GQFEAPAGSRYITGIEINTTWRENEVHVLGYNVPLGPSAINELLEYNQGQRRTRAQTM
                     VDQIRRAGYSLTIDDVLRESDAADSVGRPHVAKALVRAGMTESVDTAFRDILGRGKPG
                     YVPQLYTTPQRAIEEIANAGGIPVLAHPGRLKDRVLIDDLAGHGLRGLEVFYPLHDAA
                     DVAEFREKARKYGLVMTAGMDFHDIRYHTRGVGMEIDDADIAPFLELVEVFA"
     gene            complement(4059..4688)
                     /locus_tag="VFU90_00455"
     CDS             complement(4059..4688)
                     /locus_tag="VFU90_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEU5478264.1"
                     /translation="MESIEEYLASLASDAPVPGGGSAATLVAATGAALVGMVARICAG
                     NPKYAAGAALTARLAAESDRLRTELSAARERDERAFAAVVAAQALPKAAPEEKASRER
                     ALEDALRAAAREPLEAAALALAVVRCAEQLLEIPNRNLASDVGCAAEFGFAALAACAY
                     NVRVNHRYMRDPASIEAQAAQLDGYEREGAATLARVRAAVAQLLAPARA"
     gene            4813..>5845
                     /gene="ahcY"
                     /locus_tag="VFU90_00460"
     CDS             4813..>5845
                     /gene="ahcY"
                     /locus_tag="VFU90_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5478265.1"
                     /translation="MPVLDRTAGDVKDRGLAEAGRSRIAWAAAYMPVLAQIHDRFATE
                     KPLAGVRIGACLHVTTETANLMLALSAGGAEVALCASNPLSTQDDVAAALCDYGIPTY
                     AIKGEDNATYYAHIESVIAAKPQMSMDDGCDLVTTIYSKHNAMLADMIGGCEETTTGV
                     IRLRAMQKDGLLPYPVIAVNNALTKHMFDNRYGTGQSTLDGIVRATNVLLAGRTLVVA
                     GYGWCGRGIASRASGMGAHVVVTEVDPRKAIEAQMDGYRVMPMADAAKIGDVFVTVTG
                     NYHVIREEHFKLMKDGAIVCNSGHFNDELDLDGIKRISRGAAAPREFVEQYELHDGRK
                     ICILADGRLV"
BASE COUNT         1026 a         1891 c         2033 g          895 t
ORIGIN      
        1 gttgacgaac agctcgctcg cgccgaagcg cgcggccgtt tcgaggctgc cgattccttg
       61 cgactggttg ccgaatccgc cggtcattgc gccgtagaga ccctcgggaa gatcggcggg
      121 ccggttggtg atgatgttga cgacgccgcc catccggtcg ccgccgtatt cggcgggaat
      181 cgcgccggtg agcaattcga ccgaatcgat gttcttgggg tcgacgattt cggcgaagtt
      241 cgaagtcgtt gccagcggta agggcgcgcc gtcgatattg taggttacgc cgtggaaacc
      301 gttgatgacc ggctcgttga acgaaaacgg aattacgccg ggcagggtcg cgagcaattt
      361 atccaggctg ttctgttgcg gcgacgcttg gatgttcgtc cggctcaact ggttcaccga
      421 cggtgggttg gcttgcaggc cggctcgcgc ggtcacggtg gtttgtgcga tttgtttgag
      481 gtgcgtggtt aacggaaggt cgacggtggc gaccgtgttg ctcgacaccg tcacgaaggt
      541 ttgcagcggc gggagccctt tgacttcggc ggcgagccgg tattggccga acggaacctg
      601 cggaaaaccg tacgtcccgc cggccgcggc ggtggtgcta aagcgggagc cctcgccctc
      661 gagggtgacg tgcgctccgg cggcaggctt gccgtcgacg gtgacggtgc cgcgcaccag
      721 accgtcggtc gccgcccagg cggcggatgc gccggcgaga tacaccaaaa acacggcgac
      781 gagcgccgta atgaaacgaa gcatggctct ccttcgatcg gattagcgct gagtgctaac
      841 ggagggagcg cgcagctacg cgcgcgcgcc cgcgatcgga ggaggtcgag cgaagcgacg
      901 ccgcaggagc ggcgttcggt agggtgccgg cgcgatgcgg atcgctgcgg cgcgaaccgg
      961 aaggcgcggt gcgaaggtca ggcgcgcgag ccggaggatg gcccgggcca gcaaccggac
     1021 ggccgcgccc gcaacccggc ggacaaccca ggcggccgcg cccagcgcga ccagcgtgac
     1081 cagcagaccc gccgccgagt gcggcgagtc gaagcgttcg ccgagggcga accaggcggc
     1141 ggtgccggct acgagcccgc cccagcttgg aatgcaggcc gtcatccggg cggcaaggat
     1201 gctcccatcg gcagtccggc gggcaccatt ccaagtgagg gcggccacgg cggctgcgaa
     1261 agccacggtc ccgccggcgg cgaggtcgag gagcagctcg tggtaggcgc cgcccatcgc
     1321 gtgcccgccc ccgtagagcg cgatatgcgc aagcaaggaa gcgagcaatc caagggccag
     1381 ggcgcccgga atctcccggg cggtcacgcg ggttcttttc tacaggggga cgcggggagg
     1441 cctggaggcg gcgtcgtact gtcgtgaccc cgcacctggc cgctcgttac ctcggtggga
     1501 atgggagtct cgcaatgaac agctggtctt cgctatgagc ctgcgcaccc gggttgaaaa
     1561 cgccgatacg gtgagcctgg tgtgcgcgct gctcgtgcgc ttcccggagc ttgcctcgat
     1621 ccgctcgatg ccggtggacc ggacggtgcg gcttaccttc gcgttgcgcc agcggctcga
     1681 ccgccacgcg caggcggcgc tcaccgaagc gctcgacgac cacatccgaa gcttcctggc
     1741 gctcttcggt gacgatcccg aaacgttgcg cttcgaatgc gaaagcgacc ggtcgatgac
     1801 gttcgtgcac atcacccgcg atctcgagag ctttactaaa gaagagctgg agcttcaggt
     1861 ggcgttcttc ggcgagcggt tcagcgacgc gctggtgcgc aacccggttc ccgacgacca
     1921 cgtcgacgcc gatccggcgg cgcaggacga agccgcgctg ttcgcggtcg aggcgctgcg
     1981 cgacccggcg cagtccaaga gtctggtcgg attccgagaa gagaagcgag tgctcgtgta
     2041 tttcctgaag tcgcagaaga aagccaaggc gtcggcgcgc cgatagcacg aatcaacctg
     2101 ctgctggttg gggacgtggt cggttccccg gggcgcgaca ccctcaaacg ctgcgtcgcc
     2161 ctggcgcgcg aacagcacca tatccacgct tgcctggcca atggcgaaaa cgccgcggcc
     2221 ggctttggtt tgacggcgca aaccgccgaa gagatgttcg aaagcggggt cgacttcatc
     2281 accagcggca atcacatctt cgacaaacgc gactttcgcg aatacctcga aaccagcgac
     2341 cgggtgatcc ggccggccaa ctatccgccg accgccccgg ggcgcggcag cggtacgctg
     2401 gcggtcgacg gcgtgaagat cgggatcctc aacctcatgg ggcggacctt catgccgccg
     2461 gtggacgacc cgtttcgctg cgccgacgcg ctgctcgagg atttgcagcg cgacgctcgg
     2521 gtgatcgtgg tggacatgca cgccgaagcg acctccgaaa aggtcgcgat gggccgctat
     2581 ctcgacgggc gcgtatcgtg cgtgttcggg acgcacaccc acgtccagac ctccgacgag
     2641 cagatcctgc cgggcggcac ggcctacatc acggacgtcg ggatgaccgg cccgacggac
     2701 ggcgtcatcg gcatggaatc gcgcaccgtg ctggatcgct tcctcaatgg ggtctcggag
     2761 cggttcaacg tccagaagac cgggagcaag cagttttgcg ccgcggtggt agcgatcgac
     2821 gccgactcgg ggaaagcgat cgaaatcaag cgcatctaca cccgcggaat cgcgtgaggt
     2881 tccctcgatg agcgccgacg gtccgctgaa agtctccgcc aagagcaatc ccaacgccgt
     2941 cgccggcgcg ctggcggcaa ccctgcgcga gcgcgagagc gccgagcttc aggcggtcgg
     3001 cgcgggcgcg atcaatcaag cgatcaaagc ggtggcgatc gcgcgcaact acctcaagcc
     3061 cagcggcatc gacgccgtct gcgtgccggc tttcatcacg gtggcgatca gcgacatcga
     3121 gcgcaccggc atctcgctcg tcatcgagcg gcgggcgctg ccgccgccgt ccgagcagga
     3181 cacgaccggc gctgcgccac acacacgcga cgaaccgatc gacgacgcca ctccgggaga
     3241 cgtttcctcg tgattatcga cttccactcg cacagcaaca tgagcgacgg gacgctgacc
     3301 ccgcaggcgc tcgcggattt catgggcgag cggaaggtcg agatgttctg catctccgat
     3361 cacgacacgc tgagcgcgta cggtcagttc gaggcgcccg cgggctcgcg ctacatcacg
     3421 gggatcgaga tcaacaccac ctggcgcgag aacgaagtgc acgtgctggg ctacaacgtg
     3481 ccgctcgggc cttcggcgat caacgagctg ctcgaataca atcagggcca gcgccggacg
     3541 cgcgcgcaga cgatggtcga tcaaatccgg cgggcgggct actcgctgac gatcgacgac
     3601 gtgctgcgcg aatccgacgc ggccgattcg gtcgggcggc cgcacgttgc caaggcgctg
     3661 gtgcgagcgg gaatgacgga gagcgtcgac acggcgtttc gcgacatcct cggtcgcggc
     3721 aaaccgggct acgtgccgca gctgtatacg acgccgcagc gcgcgatcga agagatcgcc
     3781 aacgcgggcg gaattccggt gctggcgcat cccgggcggt tgaaagaccg ggtgctgatc
     3841 gacgacttgg ccggccacgg tttgcgcggg ctggaggtat tctatccgtt gcacgacgcc
     3901 gccgacgtcg ccgagttccg ggagaaggcg cgcaagtacg gcctcgtcat gacggccggg
     3961 atggacttcc acgacatccg ctaccatacg cgcggcgtcg ggatggagat cgacgacgcc
     4021 gacatcgcgc cgtttctcga actagtcgaa gtcttcgctt aagcgcgcgc aggcgcgagc
     4081 agttgggcga ccgcggcccg cacgcgagcg agcgttgccg cgccctcgcg ctcgtagccg
     4141 tcgagttgcg cggcttgcgc ctcgatcgac gccgggtctc gcatgtagcg gtggttgacg
     4201 cgcacgttgt acgcgcacgc ggccagcgcg gcgaatccga actcggcggc gcagccgacg
     4261 tcgctggcta agttgcggtt gggtatctcc aacagctgct cggcgcaacg caccaccgcc
     4321 aacgccagcg ccgcggcttc gagcggctcg cgcgcggcgg cgcggagcgc atcctcgagc
     4381 gcgcgctcgc gcgacgcttt ctcttcgggt gcggctttgg gaagagcttg cgccgcgacg
     4441 acggccgcga aggcacgctc gtcgcgctcg cgcgccgccg agagttcggt tcgcagacga
     4501 tcgctttccg cggcgagacg cgcggtcagc gcggcgccgg cggcgtattt tggattgccg
     4561 gcacagatgc gggcaaccat tccgactaaa gcggcaccgg tcgccgcgac cagcgtcgca
     4621 gcgctcccgc cgcccggcac cggcgcgtcg gaggcgaggc tcgccagata ttcctcgatg
     4681 ctctccacgt gcgctccagt taggcagcgc ggcggccgcg acctcgaccg cgcgcaaccc
     4741 cggcggaggt agccgggtct gcgccgaaag cgcccgcgag cgcttcgcac ccacggatga
     4801 gaggtagaaa ctgtgcccgt attagatcgt accgccggcg acgtcaagga tcgcggcctc
     4861 gcggaggccg gccgttcgcg gattgcgtgg gcggcggcgt acatgccggt gttggcccaa
     4921 attcacgatc gcttcgccac cgaaaagccg ctggccggcg tgcgcatcgg cgcctgtctg
     4981 cacgtcacga ccgagaccgc caacctcatg ctggcgctgt cggcgggcgg cgcggaggtg
     5041 gcgctgtgcg cgagcaaccc gctatcgaca caggacgacg tggcggcggc gctgtgcgac
     5101 tacggcatcc ccacctacgc gatcaaaggt gaagacaacg cgacctacta cgcgcacatc
     5161 gaatcggtga ttgcggccaa gccgcagatg tcgatggacg acggctgcga cctcgttacg
     5221 acgatctaca gcaaacacaa cgcgatgctg gccgacatga tcggcggatg cgaggagacg
     5281 accaccggcg tgatccggct gcgcgccatg cagaaggacg ggttgctgcc ctacccggtg
     5341 atcgccgtca acaacgcgct caccaagcac atgttcgaca accgttacgg caccgggcag
     5401 tcgacgctcg acggcatcgt gcgcgccacc aacgtcttgc tggcgggccg cacgctggtg
     5461 gtggcgggtt acggctggtg cggccgcggg atcgcttcgc gcgcgtccgg gatgggcgca
     5521 cacgtggtcg ttaccgaagt cgatccgcgc aaggcgatcg aagcgcagat ggacggctac
     5581 cgcgtgatgc cgatggccga cgcggccaag atcggcgacg tgttcgtgac cgttaccggc
     5641 aactaccacg tgattcgcga agagcacttc aagctgatga aggacggcgc gatcgtctgc
     5701 aactcggggc atttcaacga cgagctcgac ctcgacggca tcaaacggat cagccggggc
     5761 gcggccgcgc cgcgcgaatt cgtggagcaa tacgagctgc acgacggccg gaagatctgc
     5821 atcctcgccg acgggcggct ggtga
//
LOCUS       DASUCJ010000041         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1238477, whole genome shotgun sequence.
ACCESSION   DASUCJ010000041 DASUCJ010000000
VERSION     DASUCJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFU90_00465"
     CDS             complement(<1..380)
                     /locus_tag="VFU90_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5478266.1"
                     /translation="MSQTQAPPKRVDVVGVPMDLGASRRGVDMGPSAVRYAQLHAKLR
                     RLGIESIVDHGNLDVPIRESASLDDAKAKYLNVIQDVCGRLAEVVEKAVRDGGVPVVL
                     GGDHSIAMGTLAGLGRAYGAPPGLV"
     gene            complement(377..>1171)
                     /locus_tag="VFU90_00470"
     CDS             complement(377..>1171)
                     /locus_tag="VFU90_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5478267.1"
                     /translation="VGTFVTRYPELSARVSDVIYNPSNGLAARLPDWMRHEFARIPDE
                     LAAWVKTRGLASFGRIVVVLAGGFAAVATFVIVPLLTAYLLLDLDNLKAELASIVPPR
                     RWRSMVEVLAEIDAVIGGFIRGQLLVAISVGVLITIALLLLHVRYAFLFGLLAAIGDL
                     VPYVGAILAFVPAFTVAWIGNGIVNAMFVLAAFVLIFQAEGHLLAPNIVSKTVKLSPF
                     VVLLALLIGGELGGIFGLFVAIPIAGILRLFVTRVFRPKPANEPSP"
BASE COUNT          240 a          394 c          365 g          172 t
ORIGIN      
        1 accaatccgg gcggcgcccc gtaggcccgc ccgagtcccg cgagcgtgcc catcgcgatc
       61 gagtgatctc cacccaagac gaccggcacc ccgccgtcgc gtacggcttt ctcgacgacc
      121 tcggccagcc gtccgcagac gtcttgtatg acgttaaggt attttgcctt cgcatcgtcg
      181 agcgacgccg attcgcgaat cggtacgtcg aggtttccgt ggtcgacgat cgattcgatg
      241 cccaagcgcc gcagcttggc atgcagctgc gcgtagcgca cggccgacgg gcccatatcg
      301 accccgcggc ggctcgcgcc gagatccatc ggcacgccga cgacgtccac gcgtttcgga
      361 ggcgcttgcg tctgactcac ggtgacggct cgttcgcggg cttcggccga aagacccgcg
      421 tgacgaacag ccttaaaatc ccggcgatcg ggatggcgac gaacaaaccg aagataccgc
      481 ccagctcgcc gccgatcagg agtgcgagca gcaccacgaa cgggctgagc ttgacggtct
      541 tgctcacgat gtttggggcg agcagatggc cttcggcctg aaatatcaaa acgaacgccg
      601 cgagcacgaa catcgcgttg acgattccgt ttccgatcca cgccaccgtg aacgccggca
      661 cgaacgccag gatcgcgccg acgtaaggaa cgagatcgcc gatggcggcg agcagaccga
      721 acaagaatgc gtaccgcacg tgaagcaaga gcagggctat cgttatcaga acgccgaccg
      781 agatcgcgac cagcagctga ccgcgaatga agccgccgat caccgcgtcg atctcggcaa
      841 gcacttccac catcgaacgc cagcgccgcg gcggaacgat ggatgccagc tcggccttca
      901 agttgtcgag gtcgagcagc agatacgcgg tgagcagcgg gacgatgacg aaggtcgcga
      961 ccgcggcgaa accgccggcc agcaccacga cgatgcgacc gaagctcgcc agcccacgcg
     1021 ttttcaccca cgccgcgagt tcgtcgggaa tgcgggcgaa ctcgtggcgc atccaatcgg
     1081 gcagccgcgc cgccaatccg ttgctcggat tgtagatcac gtcgctcacg cgcgccgaca
     1141 gctcggggta gcgcgtgacg aacgtgccga c
//
LOCUS       DASUCJ010000042         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_76895, whole genome shotgun sequence.
ACCESSION   DASUCJ010000042 DASUCJ010000000
VERSION     DASUCJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76895"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFU90_00475"
     CDS             complement(<1..764)
                     /locus_tag="VFU90_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478268.1"
                     /translation="MRLQHVSPGFDPSGVISMRLGATARQFANRDAALAYWATFGEQL
                     ASVPGVKERGAVSALPFTSSVGWGTINVEGWTPEPGQELQVDVRGATPGYFSTMRIPL
                     VAGRYFSDADLSESSAPVAIIDKKFAQRFWPHGNAIGKHVWFGSAPTKNSLTIVGVVG
                     TVKQYGLDVDGRIVVYGPSANAGYQVARTSGDPATVAGAMVKKIHQLDPTLTVFDVRT
                     MTDRMSDSMARQRFSTTMLGAFAGFALLLAIVGVYGV"
     gene            750..>1110
                     /locus_tag="VFU90_00480"
     CDS             750..>1110
                     /locus_tag="VFU90_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478269.1"
                     /translation="MLQAHEAANEQTGADEQHDRDRHLAGDEEASQPSPRQSETAIAL
                     RVPGAGFENRIEIEPRRTQRRRQAKQNTGEQRHREGEREHVSVDADPVDEGNVPWIDD
                     PDGAESERGDEQTRDAAH"
BASE COUNT          215 a          368 c          356 g          171 t
ORIGIN      
        1 acaccgtaca cgccgacgat ggccagcagg agcgcgaagc cggcgaacgc gccgagcatt
       61 gtcgttgaaa agcgctgccg cgccatcgaa tcgctcatcc gatcggtcat cgtgcgaacg
      121 tcgaacacgg tgagcgttgg atcgagctgg tgaatcttct tcaccatcgc accggccacg
      181 gtggctgggt cgcccgacgt cctggccacc tgatagcccg cattcgccga cgggccgtag
      241 acgacgatcc tgccgtcgac gtcgaggcca tactgcttca cggtcccgac gacgccgacg
      301 atcgtgaggc tgttcttggt cggagcagat ccgaaccaga catgcttgcc gatggcgttc
      361 ccatgcggcc agaatcgctg cgcgaatttc ttgtcgatga tggcgacggg cgccgagctc
      421 tccgagagat cggcgtcact aaagtagcga ccggcaacga gcggtattct catcgtgctg
      481 aagtaccccg gtgtcgcgcc gcgcacatcg acctgtagct cctgcccagg ctccggagtc
      541 cacccttcca cgttgatcgt gccccatccg accgacgatg tgaatggaag cgccgagacc
      601 gcgcctcgct ctttcacgcc gggcacgctg gccaactgct cgccaaatgt cgcccagtag
      661 gcgagtgccg cgtcgcggtt tgcgaactgg cgcgcggtcg cgccgaggcg catcgatatg
      721 acgccggacg ggtcgaagcc cggagacacg tgctgcaggc gcacgaagct gcgaatgagc
      781 agaccggcgc cgacgagcaa cacgatcgag atcgccacct cgccggcgac gaggaagctt
      841 cgcaaccgtc gccgcgacaa tccgagaccg ccatcgccct gcgtgttcct ggcgccggat
      901 ttgagaatcg aattgagatc gagccgcgcc gcacgcagcg ccggcgccag gccaaacaga
      961 ataccggcga gcagcgacac cgcgaaggtg aacgcgagca cgtttcggtc gatgccgatc
     1021 cggtcgatga ggggaatgtt ccctggattg atgacccgga cggcgcggag agcgagcgcg
     1081 gcgacgagca gacccgcgac gccgcccatg
//
LOCUS       DASUCJ010000043         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_229776, whole genome shotgun sequence.
ACCESSION   DASUCJ010000043 DASUCJ010000000
VERSION     DASUCJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229776"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFU90_00485"
     CDS             complement(<1..245)
                     /locus_tag="VFU90_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478270.1"
                     /translation="MSSPTDPNTPRVGVDEWVASHEQRQDRRAGIAGVVQRQLARVPS
                     PAFYLAFGIAAALVPAVTSNGYNIRVGFDTLLYMLLAL"
     gene            complement(242..1177)
                     /locus_tag="VFU90_00490"
     CDS             complement(242..1177)
                     /locus_tag="VFU90_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5478271.1"
                     /translation="MNWNLFVTEIVAGVSHGSVYALIALGYSMVYGILKLLNFAHGDI
                     YMVGSFIGFGIITVFGGPLNLTVPVWLMLFIMFFCAMLGGGLLGVMVERFAYRRLRNA
                     PRIAPLITALGVSFFLENAAQLVLGAQYQSYNSFSWTNSAIALRHFGLPAHAVISYAQ
                     VIVIVGAVVLMVFLTLFVQRTQLGKAMRATSFDREAAAMMGIDVDRVIVATFFIGSAL
                     AGAAGVFNGIAYQQVWPYMGFQAGLYAFIAAVVGGIGNLPGAVLGGLLIGLAQAFSIG
                     YLSSTFSDAIVFAILIAVMIVRPSGLLGRAAVQKV"
     gene            complement(1230..>1487)
                     /locus_tag="VFU90_00495"
     CDS             complement(1230..>1487)
                     /locus_tag="VFU90_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478272.1"
                     /translation="HKQFPGATSPFGAPNYVLAQMYASAVTMACKDGKISRAEVRKDV
                     LKVRLSNTILGGPIRLTANGDVAGAKFHIFKIENGKYVTVA"
BASE COUNT          284 a          492 c          497 g          214 t
ORIGIN      
        1 agcgcgagca gcatgtacag caacgtgtcg aacccgacgc ggatgttgta gccgttgctc
       61 gtcaccgcgg gaacgagcgc cgccgcgatc ccgaaggcga gatagaacgc cggcgacggg
      121 acgcgcgcga gctggcgctg gacgacgccg gcgatcccgg cgcggcggtc ctgccgctgc
      181 tcgtgcgagg cgacccactc gtcgacgccg acgcgaggcg tgttcggatc ggtcgggctg
      241 ctcatacctt ctgcaccgcc gcgcgtccga gcagtccgct cggccgcacg atcatgaccg
      301 cgatgaggat cgcgaacacg atcgcatcgg agaacgtgga cgagagatac ccgatcgaga
      361 acgcctgggc gagcccgatc aggaggccac cgagcacggc accaggcagg ttgccgatgc
      421 cgccgacgac ggcggcgatg aacgcgtaca gccccgcctg gaagcccatg tagggccaca
      481 cctgctggta ggcgatcccg ttgaagaccc cggcggcgcc ggcgagcgcc gagccgatga
      541 agaaggtcgc gacgatgacc cggtcgacgt cgatgcccat catcgccgcc gcttcccggt
      601 cgaacgacgt cgcccgcatc gccttgccga gctgcgtccg ctgcacgaag agggtgagga
      661 agaccatgag gacgacggcg ccgacgatga cgatcacctg cgcgtagctg atgaccgcgt
      721 gcgccggcag gccgaagtgc ctgagcgcga tcgcggagtt cgtccagctg aacgagttgt
      781 agctctggta ctgcgccccg agcacgagct gcgccgcgtt ctcgaggaag aacgacaccc
      841 cgagcgcggt gatgagcggt gcgattcgcg gcgcgttgcg cagccgccgg tatgcgaagc
      901 gctcgaccat gacgccgagc aacccaccgc ccagcatcgc gcagaagaac atgatgaaga
      961 gcatcagcca caccggcacg gtgaggttca gcggcccccc gaagacggtg atgatcccga
     1021 aaccgatgaa cgagcccacc atgtagatgt cgccgtgggc gaagttcagg agcttgagga
     1081 tgccgtacac catcgagtag ccgagcgcga tcagcgcgta cacgctgccg tgcgacacgc
     1141 cggccacgat ctcggtgacg aagaggttcc agttcatccg acgagaggga gtgtgaccga
     1201 cggaccggcc acactccccg actcgggaac tacgcgaccg tcacgtactt gccgttttcg
     1261 atcttgaaga tgtggaactt cgcgcccgcc acgtcgccgt tggcggtgag ccgaatcggg
     1321 ccgccgagga tcgtgttgga cagacgcacc ttgagcacgt ccttgcgaac ctccgcccgg
     1381 ctgatcttgc cgtccttgca cgccattgtc accgcgctcg cgtacatctg tgcgagcacg
     1441 taattcggag ccccgaacgg gctcgtcgcg ccggggaact gcttgtg
//
LOCUS       DASUCJ010000044         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1421889, whole genome shotgun sequence.
ACCESSION   DASUCJ010000044 DASUCJ010000000
VERSION     DASUCJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421889"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFU90_00500"
     CDS             complement(<1..358)
                     /locus_tag="VFU90_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478273.1"
                     /translation="MAGERKRTLLSWSSGKDSAWSLHVLRQRDDVEVVGLLTTITEPY
                     DRVAMHGVRAELLRAQAEAAGLPLWPAPIPSPCSNAEYEARMGAVVRRAVDEGIRAVA
                     FGDLFLEDIRAYREGQL"
     gene            608..>1029
                     /locus_tag="VFU90_00505"
     CDS             608..>1029
                     /locus_tag="VFU90_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5478274.1"
                     /translation="MNRKWIALSWVTIAMSLAIAAFWFQQDGGRARAATERFGPKEAQ
                     VTIEDGDWKTVHLTASKFMWQFRDDQAPVEVWGYNNQIPGPTLRFKPGDKVRIYFRNE
                     LDEASSVHWHGLVVPNSMDGVGLLTMPPVMPGETFVYE"
BASE COUNT          178 a          345 c          337 g          169 t
ORIGIN      
        1 cgagctggcc ttcccggtac gcgcgaatgt cctcgaggaa caggtcgccg aacgcgaccg
       61 cgcggatacc ttcatcgacg gcgcgccgca cgaccgcgcc cattcgtgct tcgtactcgg
      121 cgttcgagca cggactgggg atcggcgccg gccacagcgg cagcccagcc gcttccgcct
      181 gtgcccgcag caactcggcg cgcaccccgt gcatggcgac gcggtcatac ggctcggtga
      241 tcgtcgtcag cagcccgacg acctcgacgt cgtcgcgctg gcgcaggacg tgcagcgacc
      301 acgcgctgtc cttcccgctg ctccacgaga ggagcgtccg ctttcgctcg cccgccatgg
      361 ccgaatcgta cggcgatgaa ggcgcacgca cgagcgggtc tcgcggcttc gcgatccgcg
      421 ttcgcacgcg cgctgcggcc agcgccccgc cggcgcattc ttgtgaagtt gccacagaat
      481 tgctcggaat tgcccaacat ttgggatcgg ctttagggcc gaacggccca tctgccaacg
      541 cgtgaaattc gtcacgatag gcgaaaccaa ttcgcaacag ttggcgagcc gtgtggagga
      601 gagacggatg aaccgcaaat ggatcgcgct gtcgtgggtg accatcgcca tgtcgctcgc
      661 gatcgccgcg ttctggttcc agcaggacgg cggccgcgcc agggccgcga ccgagcgctt
      721 cggccctaaa gaagctcagg tgacgatcga ggatggcgat tggaagaccg tccatctcac
      781 ggcgtcgaag ttcatgtggc agttccgcga cgaccaggcg ccggtcgagg tctggggata
      841 caacaaccag atcccggggc cgacgctgcg cttcaagccc ggggacaagg tccgcatcta
      901 cttcaggaac gagctcgacg aggcgtcgtc ggtccactgg cacgggctcg tcgtgcccaa
      961 ctcgatggac ggcgtcggcc tcctgacgat gccgccggtc atgcccggcg agacgttcgt
     1021 atatgagtt
//
LOCUS       DASUCJ010000045         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_520082, whole genome shotgun sequence.
ACCESSION   DASUCJ010000045 DASUCJ010000000
VERSION     DASUCJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520082"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFU90_00510"
     CDS             complement(<1..209)
                     /locus_tag="VFU90_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478275.1"
                     /translation="MLLAAFATAARCAEREPIKFPDTQYEPVEWASLDGWAGDDHAAA
                     FATFLESCRALNGGRPPSAETSTLA"
     gene            complement(257..973)
                     /locus_tag="VFU90_00515"
     CDS             complement(257..973)
                     /locus_tag="VFU90_00515"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEU5478276.1"
                     /translation="MFDIYTIIFLALAVFIFFRLRSVLGQRTGRERPPYDPYSARDAV
                     RSPANNDKIVTLPQRPAEPAASRLPAEPAPPPVDRWKDVAEAGSSVAAGLDAIVAVDQ
                     GFDAKHFITGARAAYEMIVTAFAEGDRRQLRSLLSREVYDGFDAAIGERESRGETAET
                     RFVSIDGSTITAAELRARTAQITVRFVSKLISVTRGRTGNVIDGNAEKVTDVTDVWTF
                     AREVASRDPNWKVIATEAGP"
BASE COUNT          190 a          361 c          370 g          187 t
ORIGIN      
        1 tcggcgagcg tggaggtttc cgccgacggc ggacgcccgc cgttcagcgc gcggcagctt
       61 tccagaaagg tcgcaaaggc cgcggcgtga tcgtcgcccg cccagccgtc gaggcttgcc
      121 cattcgacgg gctcatattg cgtgtccgga aatttgatcg gctcgcgctc tgcgcaacgg
      181 gcggcggtcg cgaaggcggc aagcaacaac gcaatggcga ccggaagcca cgctcgaccg
      241 aacgtcggca tggggactac ggtccggctt cggttgcgat gaccttccaa ttgggatccc
      301 gggaggcgac ttcgcgtgcg aaggtccaga cgtcggtcac gtcggtgacc ttttcggcgt
      361 tcccgtcgat tacgttgccg gtccgtccgc gcgtgaccga aatgagcttg gaaacgaaac
      421 gcaccgtgat ctgcgcggtg cgcgcccgca gctcggccgc cgttatcgtc gaaccgtcga
      481 tcgagacgaa ccgcgtctcg gcggtctcgc cgcggctctc gcgttcgccg atcgccgcat
      541 cgaaaccgtc gtagacctcg cgcgagagca agctgcgcaa ttgccggcga tcaccctccg
      601 cgaaggcggt cacgatcatc tcgtaggcgg cacgcgcgcc ggtaatgaaa tgcttggcgt
      661 cgaaaccttg gtcgaccgcc acgatggcat cgagcccggc cgccacgctc gagccggctt
      721 ccgccacgtc cttccagcga tccaccggcg gcggcgcggg ctcggcgggc aggcggctcg
      781 ccgccggttc cgccggccgc tgcggcaagg ttacgatctt gtcgttgttg gcggggctgc
      841 gcaccgcgtc gcgcgccgaa tagggatcat agggcggccg ttcgcgcccg gtgcgttgtc
      901 ccaagacgct gcgaagccga aagaagataa aaacggcgag cgccaggaag atgatggtgt
      961 aaatgtcgaa cacgtctcaa ttctctcggc gtcgcgtagg aacgatagac ccgaaggcgg
     1021 ttccaggtcg ccgaccctgg gtgctctggc cgggggtcac aaacttcccg gggtcactta
     1081 gcttatgtag gcacgcacca acgaccat
//
LOCUS       DASUCJ010000046         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1253787, whole genome shotgun sequence.
ACCESSION   DASUCJ010000046 DASUCJ010000000
VERSION     DASUCJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253787"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFU90_00520"
     CDS             complement(<1..630)
                     /locus_tag="VFU90_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5478277.1"
                     /translation="MADTLSPDVVVIGGGPGGYVAAIRAAQLGLSTVCVEMDATLGGT
                     CVNVGCIPSKALLQSSEHFEFTRTHLADHGVKVGDVTLDLPTMLTRKDRVVSANTRGI
                     EYLFRKNNVTWAKGRGTLRPGNVVEVRAADGATTTYRARDVVIATGSVPIALPFLPFD
                     EERVLSNVGALTMPEVPRHLIVIGGGVVGLELGSVWRRLGAEVTIIELLP"
     gene            complement(686..>1281)
                     /locus_tag="VFU90_00525"
     CDS             complement(686..>1281)
                     /locus_tag="VFU90_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5478278.1"
                     /translation="VDMSGVMGVRDRLKERVEKQHGVKLTFMPFFARAATMALTAYPV
                     VNAQIDGDTITYKHYVNLGIAVASDQGLVVPVIKDADRMGMIEFSRQLSAVAARARDG
                     KLTMDDLTGGTFTITNGGVFGSLVSTPILNYPQVGILGLHKIQDRPVAVGGAVVIRPM
                     MYIALSYDHRLVDGQQAVLFLVRMKELMEDPAAMLVD"
BASE COUNT          232 a          419 c          414 g          216 t
ORIGIN      
        1 cggcaagagc tcgatgatcg tgacctctgc gcctaaccgg cgccacaccg agccgagctc
       61 gaggccgacc acgccgccgc cgatcacgat gagatgtcgc gggacctcgg gcatggtgag
      121 cgcgccgacg ttggacagca cgcgttcttc atcgaacgga agaaagggca gcgcgatggg
      181 cacgctgccc gtcgcgatga cgacgtcgcg cgcccgatag gtggtggtcg cgccatccgc
      241 cgcgcgcact tcgaccacgt ttcccggacg cagcgtgccg cgtcccttcg cccaggtgac
      301 gttgttcttc cggaacagat actcgatgcc gcgcgtgttg gcggacacga cgcgatcttt
      361 gcgcgtgagc atcgtgggaa gatcgagcgt cacgtcgccg accttcacgc cgtggtcggc
      421 gagatgcgtg cgggtgaact cgaagtgctc ggacgactgc aggagcgctt tggacgggat
      481 gcaccccacg ttcacgcacg tgccgcccag cgtagcgtcc atttcgacgc acaccgtgga
      541 caggccgagc tgcgcggcgc gaatcgcggc cacgtacccg cctggaccgc cgccgatgac
      601 gacgacgtcg ggagaaagcg tatcagccat tgcctaaccg gtaaacgaga agcgcacgga
      661 cgatgttccc gcgcgccgcg cgcgcctaat cgacgagcat ggccgccggg tcttccatca
      721 gctccttcat gcgcacgaga aagagcacgg cctgttggcc atcgacgagg cgatggtcgt
      781 acgacagcgc gatgtacatc atcgggcgga tcacgacggc gccacccacc gcgaccgggc
      841 ggtcctggat tttgtgcagg cccaggatgc cgacctgcgg atagttcagg atcggcgtcg
      901 ataccagcga gccgaatacg ccgccgttag taatcgtgaa cgtcccgccg gtgagatcat
      961 ccattgtgag cttgccgtcg cgcgcgcgcg cggcgacagc cgacaattgg cggctgaact
     1021 cgatcatccc catgcgatcg gcgtctttga tcaccgggac gacgagtccc tgatccgacg
     1081 cgacggcgat gccgaggttc acgtagtgct tgtaggtgat ggtgtcgccg tcgatctgcg
     1141 cgttcacaac cggataggcc gtgagcgcca tcgtcgctgc gcgcgcgaaa aacggcatga
     1201 acgtcagctt gacgccgtgc tgcttctcga cgcgttcctt taggcggtcg cggactccca
     1261 tgacccccga catatcgact t
//
LOCUS       DASUCJ010000047         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_825773, whole genome shotgun sequence.
ACCESSION   DASUCJ010000047 DASUCJ010000000
VERSION     DASUCJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825773"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFU90_00530"
     CDS             complement(<1..395)
                     /locus_tag="VFU90_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5478279.1"
                     /translation="MHELKPAHDADPAVAAVPAAKLGRAIVVTSGKGGVGKTTTTANL
                     GTALAQRGATVALVDADVGLRNLDIVMGLESRVKHHLLDVLEERVTLDDALVRDKHSE
                     SLVLLAAAQSREKEDVDTEKLRALIGRLR"
     gene            complement(399..1190)
                     /gene="minD"
                     /locus_tag="VFU90_00535"
     CDS             complement(399..1190)
                     /gene="minD"
                     /locus_tag="VFU90_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEU5478280.1"
                     /translation="MSARRIVITSGKGGVGKTTTTANVGAALAKRGQRVILIDADIGL
                     RNLDLVLGLEKRIVFDLVEVAEGRCQLRQALIKDKRFESLSILPAAQTREKDAIDEAQ
                     FSAIVERAAEHADYVLIDCPAGIEAGFRNAVAGAAEAIVVTTPEVSAIRDADRVVGKL
                     NDRGKPIRLIVNRLRPEMVRTGDMLSVDDVCEILSVELLGIVPDDGEIIDTTNRGEPV
                     VLDESSRLRTIYDKIARRLEGEIVPFTSFDERSFFGRLIGAMKAG"
     gene            complement(1187..>1400)
                     /locus_tag="VFU90_00540"
     CDS             complement(1187..>1400)
                     /locus_tag="VFU90_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478281.1"
                     /translation="AGAQGDAAARVYALHLDATQLRIATFIAAEGERDESANNARAGA
                     ALVRDGRIAVVALDQLGKLERGVPPS"
BASE COUNT          202 a          467 c          469 g          262 t
ORIGIN      
        1 tcgcgcagcc ggccgatgag cgcgcgcagc ttctccgtgt cgacgtcttc tttttcgcgc
       61 gactgcgccg cggccagtaa gacgagcgat tcgctgtgct tgtcgcgaac cagcgcgtcg
      121 tcgagcgtga cgcgctcttc cagcacgtcg agcaggtggt gcttgacgcg gctttcgaga
      181 cccatgacga tgtcgagatt gcgcagaccg acgtcggcgt cgaccagcgc aaccgtcgcg
      241 ccgcgctgcg ccagcgccgt acccaagttg gcggtggtcg tggtcttccc gacgccgcct
      301 ttgcccgagg tcacgacgat cgcgcggccg agcttcgcag ccggcaccgc ggcgacggcc
      361 ggatcggcgt cgtgcgccgg tttgagttcg tgcatgggct accccgcttt catggcgccg
      421 atcaggcgcc cgaagaagct ccgttcgtcg aagctggtaa acggcacgat ctcgccttcc
      481 aggcggcgtg cgatcttgtc gtagatcgtt cgcaaacggc tcgactcgtc gaggacgacc
      541 ggttcgcccc gattggtcgt atcgatgatc tcgccgtcgt ccggcacgat gccgagcagc
      601 tcgacggaaa ggatctcgca cacgtcgtcg accgacagca tgtcgccggt gcgcaccatt
      661 tcggggcgta accggttgac gatcagccga atcggcttgc cgcgatcgtt gagcttgccg
      721 acgacccggt cggcgtcgcg aatcgcgctg acctccgggg tggtgacgac gatcgcttcg
      781 gccgcgcccg cgaccgcgtt acggaagccc gcttcgatcc ctgccgggca gtcgatcagc
      841 acgtagtcgg cgtgttcggc ggcgcgttcg acgatcgcgg aaaactgggc ttcgtcgatc
      901 gcgtccttct cgcgcgtctg cgccgcgggc agaatcgaca gcgactcgaa gcgcttgtcc
      961 ttgatgagcg cttgccgcag ctgacaacgc ccttcggcca cttcgaccaa gtcgaaaacg
     1021 atgcgtttct ccaggcccag cacgagatcg agattgcgca ggccgatgtc ggcgtcgatc
     1081 aggatcacgc gctggccgcg cttggcgagc gccgcgccga cgttggccgt ggtcgtcgtc
     1141 ttcccgaccc cgcccttgcc cgaagttatt acgattcgac gtgcgctcac gatggtggga
     1201 ctccccgttc gagtttgccg agctggtcga gggcgacgac ggcgatgcgg ccgtcgcgca
     1261 ccagcgccgc tccggctcgc gcgttgtttg cggattcgtc gcgttcgcct tcggccgcga
     1321 tgaaggttgc gatgcggagc tgcgtagcgt cgagatgcag tgcgtacacg cgcgcggcgg
     1381 cgtcgccctg agcgccggcg
//
LOCUS       DASUCJ010000048         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1131419, whole genome shotgun sequence.
ACCESSION   DASUCJ010000048 DASUCJ010000000
VERSION     DASUCJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131419"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFU90_00545"
     CDS             <1..456
                     /locus_tag="VFU90_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU5478282.1"
                     /translation="VCIWTPDKDLAQCVRDDRVVQVDRKSKSIRNAAGVREKFGVDPE
                     HIPDYLALTGDSADGYPGLSGIGAKTAARLINRYGALESFPLEVLGADRERALLFKRL
                     ATLRSDAPVLRDVDDLRWQGPTPAFADFVARFEHRRLGERAERAAGAAR"
     gene            620..1573
                     /locus_tag="VFU90_00550"
     CDS             620..1573
                     /locus_tag="VFU90_00550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5478283.1"
                     /translation="MDELSVPRRGFKLLAGTANKGLAEEISRCLGVDLTRVTISRFAD
                     GEIFVRIDENVRGADVFIVQPTNPPADSIMELLLLIDAARRASAARITCVMPYYGYSR
                     QDRKDQPRVAIGAKLVANLITTAGANRVLGIDFHQHALQGFFDIPVDHLYAAPVFVSH
                     YRKKQLRDLVVVAPDVGSAKMARGFAKRLNGTFAIIDKRRPTANVAEVLNVVGEVEGR
                     DCLIPDDMIDTAGTVTEAARALKSLGAKDIYVCATHALLSGPAVERLTAAPIAEVTVT
                     DTIAIPQSRRFEQLKVLSVGELLAKAIRYTHSDESVSSLFE"
     gene            1605..2273
                     /locus_tag="VFU90_00555"
     CDS             1605..2273
                     /locus_tag="VFU90_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5478284.1"
                     /translation="MTAILSASKRTDTGKGAARKLRAAQMIPGVIYGHSREPLSLAMS
                     SRELGRLLERIAAETTVVELNIDGTVSRTLIREIQRHPYKREVLHVDFLELVAGEKVT
                     VDLPIVLVGTPEGVRSSGGILDQIMREISVEVDPVNMPTHLELDVSALDINDSLHVSD
                     LKVPDGVEVLEDAEATVCVVSPPHVEEEPAAPEAGAEGEPVAEPELIRKPKGEEEEGA
                     EEEK"
     gene            2321..2905
                     /gene="pth"
                     /locus_tag="VFU90_00560"
     CDS             2321..2905
                     /gene="pth"
                     /locus_tag="VFU90_00560"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5478285.1"
                     /translation="MKLIVGLGNPGREYERTRHNVGWWVVDHLADVWHLGSWRADGQA
                     AAASGRLGAAHVTLLKPLTYMNLSGHALVPYLRRPTFDPAADLLVVVDDVALPVGRIR
                     LRANGSAGGHNGLKSIEHEIGSRDYGRLRIGVRPVDPERRLGELADFVLSPFGSADRD
                     VIVSLYPQITGAVETWVRDGIAAAQNRYNADATA"
     gene            2937..>3369
                     /locus_tag="VFU90_00565"
     CDS             2937..>3369
                     /locus_tag="VFU90_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5478286.1"
                     /translation="MLSLAIVGLPNVGKSTLFNALTAARADAANYPFCTVEPNVGIVE
                     VPDPRLAVLAAIVQPKRIVPAVVQFVDVAGLVKGASSGEGLGNKFLANIRETDAIVHV
                     VRCFDDPDVQHVMGSVDPVRDRDVINLELALADLAAVEKRLE"
BASE COUNT          563 a         1073 c         1163 g          570 t
ORIGIN      
        1 gtgtgtatct ggacgccgga caaggatctc gcccaatgcg tacgcgacga tcgcgtggtg
       61 caggtggacc gcaagagcaa gtcgattcgc aacgcggcgg gcgtgcgcga gaaattcggc
      121 gtcgatccgg agcacatccc cgactatctg gcgctgaccg gcgactctgc cgacggatat
      181 ccggggctct ccggcatcgg cgccaagacg gcggcgcgtc tcatcaaccg ctacggcgcg
      241 ctggagtcct ttcctctcga ggtgctcggc gccgatcgag agcgagcgct gctcttcaag
      301 cgactggcga cgctgcgcag cgatgcgccg gtgcttcgcg acgtggacga tctccggtgg
      361 cagggaccga cgcccgcctt tgccgatttc gtggcgcggt tcgagcatcg gcgattgggc
      421 gagcgggccg agcgcgcggc gggagcagca cggtagcgcc ctggctacgc gttcctatag
      481 attcaccggc tctacacggc caactttcgg agagcgcggt gatttgcacg cttgcccaca
      541 caggttcgct gctctggtcg agatggcgtg acgcgattga ccaacttgca aggctgcttt
      601 atgttacggg gctgcgctca tggatgagct gagcgttccg cggcgcgggt tcaagctgct
      661 cgcggggacg gcgaacaagg gactcgccga ggagatctcg cggtgtttgg gcgtcgatct
      721 cactcgggtg acgatctcgc gattcgccga cggagaaatt ttcgttcgca tcgacgagaa
      781 cgtgcgcggc gcggacgtgt tcatcgtgca gccgaccaat ccgccggcgg attcgatcat
      841 ggagctgctg ctcctgatcg atgccgcgcg ccgggcgtct gcggcacgca ttacgtgtgt
      901 gatgccgtac tacggctact cgcggcagga tcgaaaggac cagccgcggg tggcgatcgg
      961 cgcgaagctg gtggcgaatc tgatcacgac cgcgggtgcg aaccgcgtgt taggcatcga
     1021 tttccaccag cacgcgttgc aggggttctt cgacattccc gtcgatcacc tctacgccgc
     1081 gccggtgttc gtgtcgcact atcggaagaa gcagttgcgc gacctggtcg tggtggcgcc
     1141 ggatgtcgga tcggcgaaga tggcgcgggg attcgcgaag cgcttgaacg gcacgttcgc
     1201 gatcatcgac aagcgccgcc caacggccaa cgtcgccgaa gtcttgaacg tggtgggtga
     1261 ggtggaaggc cgggattgtc tcattcccga cgacatgatc gatacggcag gcacggtcac
     1321 cgaggcggcg cgcgcgctca agtcacttgg cgccaaggac atttatgttt gcgcgacgca
     1381 cgcgctgctc tcgggaccgg ctgtcgagcg cctaacggcc gcgcccatcg ccgaagtgac
     1441 ggtcacggac acgatcgcga tcccgcagtc gcgtcgcttc gagcagctca aggtgctgtc
     1501 ggtgggagaa ctgttggcga aggccattcg gtacacgcat tcggatgaat cggtgagctc
     1561 gctgttcgag tgacaccagg cacccagtca cgagtgatac gcatatgacg gcaatcctct
     1621 cggcttccaa gcgcaccgac accggcaagg gcgcggcgcg gaagcttcgc gcggcgcaga
     1681 tgatccccgg cgtcatctac ggacacagcc gcgaacccct ctcgctcgcc atgagcagcc
     1741 gcgagctcgg ccgcctgctc gagcgcattg cggcggaaac caccgtcgtc gagctcaaca
     1801 tcgacggcac cgtgtcgcgc acgctcattc gcgagatcca gcggcacccg tacaagcgcg
     1861 aggtgctgca cgtcgacttc ctcgagctgg tggccggcga gaaggtcacg gtcgatcttc
     1921 cgatcgtgct cgttggaacg ccggaaggcg tgcgcagctc gggcggcatt ctcgaccaga
     1981 tcatgcgcga gatcagcgtg gaagtggacc cggtgaacat gccgacccac ctcgagctcg
     2041 acgtctctgc gttggacatc aacgactcgc tgcacgtgtc cgacctcaag gtcccggacg
     2101 gcgtcgaagt gctggaggac gccgaggcca ccgtgtgcgt cgtgtcgccg ccgcacgtcg
     2161 aggaagagcc ggccgcgccc gaggcgggcg ccgagggcga gcccgtggcg gagcccgagc
     2221 tcatccgcaa gccgaagggg gaggaggaag agggcgccga agaggagaag tgatcctggc
     2281 cggcgtgacc gtcgccggct cctgatcgcg atcgcggcgc atgaagctga tcgtcggcct
     2341 gggcaatccg gggcgcgaat acgaacggac acgccacaac gtcggctggt gggtggtgga
     2401 ccacctggcc gacgtttggc atctcgggtc ctggcgcgcg gacggacagg cggccgccgc
     2461 atcgggccgg ttaggcgcgg cgcacgtgac gctgctcaaa ccgctcacgt acatgaatct
     2521 gagcggacac gcgctggtgc cgtacctgcg ccgtccgacg ttcgacccgg cggccgactt
     2581 gctggtcgtc gtggatgacg tcgcgcttcc ggttgggcgg attcgactgc gggccaacgg
     2641 cagcgccggt ggacacaacg ggctcaagag catcgagcac gaaatcggct cgcgcgacta
     2701 cggccgcctg cgcatcgggg tgcggccggt ggatcccgag cgccgcctcg gcgagctggc
     2761 ggacttcgtg ttgtcgccgt tcggatcggc cgaccgggac gtcatcgtgt cgctctatcc
     2821 gcagatcacg ggcgccgtcg agacctgggt ccgcgacggg atcgcggccg cccagaaccg
     2881 ctacaacgcg gacgcgacgg cctgaggttg gttcaccctt ctttcgttca cgctgcatgc
     2941 tctcgctcgc catcgtcggg ctgccgaacg tcggcaagtc cacgctcttc aatgcgctca
     3001 ccgcggccag ggccgacgcg gcgaactatc cgttctgcac cgtcgagccc aacgtcggca
     3061 tcgtcgaagt gccggacccg cgcctcgccg tgctggcggc gatcgtccag cccaagcgca
     3121 tcgtcccggc ggtggtccag ttcgtggatg tcgcggggct ggtgaagggc gcgtcgagcg
     3181 gcgaagggtt aggcaacaag tttctggcca acattcgcga gaccgacgcg atcgtgcacg
     3241 tcgtgcgctg cttcgacgac cccgacgtgc agcacgtcat gggctccgtc gatccggttc
     3301 gcgatcgcga cgtgatcaac ctggagctcg cgctggccga ccttgccgcc gtcgaaaagc
     3361 gcctggaga
//
LOCUS       DASUCJ010000049         1276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_780000, whole genome shotgun sequence.
ACCESSION   DASUCJ010000049 DASUCJ010000000
VERSION     DASUCJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780000"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>1276
                     /locus_tag="VFU90_00570"
     CDS             12..>1276
                     /locus_tag="VFU90_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478287.1"
                     /translation="MTTYHGGPLRLGWDQHERQLTTTNVADEFGLLQTLTVDGNVLAQ
                     PLYLEHFAPLGGRNVVIVATEHASIYEFDADTGAQINMVNFGPSQSSRDVGCLDIRPE
                     YGITGTPAIDLSTQTMYVVVASEPSKNVFKTRLHALDIATLADKITPVDITASETISN
                     GTQLKFDPQDQMSRSSLVLANGSIYVPIGSHCDANASAISGWLLRYDSGLHQIGAFAT
                     IEDSTNYLLSSIWMTGFAPAVDADGSLFAVTGNGAFDANMGGKNYGESVLRLSPDLTT
                     DIDYFTPSNWSSLNGGDTDFGSGGIMLLPTQQGSVPHVAVAQGKFSTLYLLNRDDLGH
                     VHTGDTGALQHISGTGGGVWGGPAYYGGPTGQFVYYQAGGAPLSAYAVVNNSQGVPQL
                     QLSSTGPSYAGYGGSLPVVSSSLQKPGTG"
BASE COUNT          244 a          440 c          391 g          201 t
ORIGIN      
        1 cggcgaacga cgtgacgacg taccacggcg gaccgctccg gctcggctgg gatcagcacg
       61 agaggcagct gactacgacg aacgtcgcgg acgagttcgg gctgctccaa acgctgaccg
      121 tcgacggcaa cgtcctcgcg caaccgctct atctcgaaca cttcgctccg ctcggcgggc
      181 gcaatgtcgt catcgtcgcc accgaacacg cgtcgatcta cgagttcgac gccgacacgg
      241 gcgcgcagat caacatggtc aacttcggcc cgtcgcagag ctcgcgtgac gtcggctgcc
      301 tcgacatccg gcccgagtat gggatcacgg gcacgccggc catcgacctc agcacgcaga
      361 ccatgtacgt cgtcgtcgcg tccgagccgt cgaagaacgt cttcaagacg cgcctacacg
      421 cgctcgacat agcgacgctc gccgacaaga tcacgccggt cgacatcacc gcgtcggaga
      481 cgatcagcaa cggcacgcag ctcaagttcg atccgcagga ccagatgagc cgctcgagcc
      541 tcgtgctcgc gaacggatcg atatacgtgc ccatcggatc gcactgcgac gccaacgcaa
      601 gcgccatatc cggctggctt cttcgttacg attccggtct ccatcagatc ggcgcgttcg
      661 cgacgatcga agactcgacg aactacctgc tcagcagcat ttggatgacc ggcttcgctc
      721 cggcagtcga tgccgacggc agcctcttcg cggtcaccgg caacggcgcg ttcgacgcga
      781 acatgggcgg caagaactac ggcgaaagcg tgctgcggct gagccccgac ctcacgacgg
      841 acatcgacta cttcacaccg tcgaactggt cgtcgctcaa cggcggcgac acggatttcg
      901 gatcgggcgg catcatgctg ctcccgacgc agcagggctc ggtgccgcac gtcgcggtcg
      961 cgcagggcaa gttctcgacg ctctatctgc tgaaccgaga cgatctcggc cacgtgcata
     1021 cgggcgacac cggcgcgctg cagcacatca gcggcacggg cggcggcgtg tggggcggcc
     1081 ccgcatacta tggcgggccg acaggccagt tcgtctacta tcaggccggc ggcgcgccgc
     1141 tctccgcata tgccgtcgtc aacaactcgc aaggcgtgcc gcagctgcaa ctgtcgtcga
     1201 ccggtccgag ctacgcaggc tacggcggat cgctaccggt cgtatcatcg tcgctccaga
     1261 aaccgggtac ggggat
//
LOCUS       DASUCJ010000050         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_413205, whole genome shotgun sequence.
ACCESSION   DASUCJ010000050 DASUCJ010000000
VERSION     DASUCJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413205"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..>1024
                     /locus_tag="VFU90_00575"
     CDS             112..>1024
                     /locus_tag="VFU90_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEU5478288.1"
                     /translation="MTAEEIAQIKDEIVNESLGLGPLEDLMADPAVTEIMVNGPKTVY
                     IERSGKIERTGKRFTSAQQLRLVIERIIAPLGRRLDESAPMVDARLPDGSRVNAIIEP
                     LAIDGASVTIRRFGTRRLTSQDLLDKGSANEQMLDFLRACIEARLNIVISGGTGSGKT
                     TFLNILSSYIPDRERIVTIEDAAELYLNQPHVVRLESRPANLEGRGEIKIRDLVRNSL
                     RMRPDRIVVGECRGGEALDMLQAMNTGHDGSLTTAHANSPRDALSRLETMVMMAGFDL
                     PVRAIREQIASAVDLIIQTARLRDGSRK"
BASE COUNT          214 a          336 c          309 g          165 t
ORIGIN      
        1 aatctcgtcg aagcgagcgc cgcgcacagc gacgccgcga aactcgccga gctgcgtcag
       61 aaaatcgaag aggccgcgca ggccgttttg agcgagaaat cgcacgggga tttgaccgcc
      121 gaagagatcg cgcagatcaa agacgagatc gtcaacgagt cgctgggtct aggaccgctc
      181 gaagatctca tggcggatcc ggccgtgacc gagatcatgg tgaacggccc gaagactgta
      241 tacatcgaac gcagcggcaa gatcgagcgc accggcaaac gcttcacgag cgcgcagcaa
      301 ctgcgtctgg tcatcgaacg catcatcgcg ccgctgggcc gccgcctgga cgaatcggca
      361 ccgatggtcg atgcgcgcct gcccgacggc tcgcgcgtga acgccatcat cgaaccgctc
      421 gccatcgacg gcgcttcggt cacgattcgc cgcttcggca cccgccgtct tacctcacag
      481 gatctgctcg acaaaggatc ggccaacgag cagatgctcg attttctgcg cgcctgcatc
      541 gaggctcgtc tgaacatcgt catcagcggc ggtaccggtt ccggtaagac cacgttcctc
      601 aacattctct cgagctatat cccggaccgc gaacgcatcg tcacgattga agacgcggcc
      661 gaattgtact tgaatcagcc gcacgtcgta cggctcgagt cccgtccggc aaacttggaa
      721 ggccgcggcg agatcaagat ccgcgacctg gttcgcaact cgcttcgtat gcgccccgat
      781 cgcatcgtcg tcggcgagtg ccgcggtgga gaagcgctgg acatgctgca ggccatgaac
      841 accggccacg acggctcgct cacgacggcg cacgcaaact cgccgcgcga cgcgctctcg
      901 cgtctggaga cgatggtcat gatggccgga ttcgacctgc cggtgcgcgc cattcgcgag
      961 cagatagcaa gcgcggtcga cctcatcatt caaaccgcgc gcctgcgtga cggcagccgc
     1021 aaga
//
LOCUS       DASUCJ010000051         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_535494, whole genome shotgun sequence.
ACCESSION   DASUCJ010000051 DASUCJ010000000
VERSION     DASUCJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535494"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFU90_00580"
     CDS             complement(<1..1076)
                     /locus_tag="VFU90_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5478289.1"
                     /translation="MLTPRIAAENPNTNTFTPLASSLEDHVSARARSGLLMLAFGVGV
                     VMLIVCANLSNLLLARGTTRQKEMAIRAALGAGRRRLVRQMLTESVTLSLAGGAVGLA
                     LAVFGTRAIAHMDAVSLPLLGRVAVDGSALAFTIVVAVAAGLAFGLVPALQISEGRVH
                     DTLKSSGRSATVGKQGQLVRRSLVVSEIALACVLLVASGLLLRSFRNVLDVDLGFRPD
                     HVIAVRVDPDRETRFDSLSQAASYVDEVLRLTRQIPGVTAATIADGLPLGSNRSWGIK
                     LGAEEYRKGKWVTGFIRVATDGFVDAMGMRMVAGRDISPTDVGSSDNVVVINETTAKT
                     FWPGRSALGKLLNVGGTDRRVVGVV"
BASE COUNT          198 a          401 c          343 g          161 t
ORIGIN      
        1 acgacaccga ccacccgtcg gtccgtgccg ccgacattga gcagcttgcc caaggcactc
       61 ctgccaggcc agaacgtctt cgccgtcgtc tcgttgatca cgacgacatt gtcgctggag
      121 ccgacgtcgg tcggcgaaat gtcccggccg gcaaccatgc gcatccccat tgcgtcgacg
      181 aagccgtccg tcgccacgcg aatgaagccc gttacccact tccctttcct gtattcctcg
      241 gcacccaact tgatccccca gctgcgattg ctccccagcg gcagtccgtc ggcgatcgtc
      301 gccgccgtca cgccgggaat ctggcgggtc aaccggagaa cttcgtcgac gtacgaggcg
      361 gcctgcgaca gcgaatcgaa tcgggtctcg cggtccggat cgacgcggac ggcgatgaca
      421 tgatcgggtc ggaagccgag atcgacatcc agcacgtttc ggaagctgcg cagcaggagg
      481 cccgaggcaa ccagcaggac gcacgcgagc gcgatctcgg acacgaccag cgagcgccgc
      541 accaattgtc cctgcttgcc gacggtcgcc gagcgtcccg atgacttgag cgtatcatga
      601 actcgaccct cggagatctg gagcgccggc accagcccga acgcgagacc cgccgccacc
      661 gccacgacaa tcgtgaacgc gagcgcgctc ccgtccaccg cgacgcggcc gagcagcggc
      721 aggctcacgg catccatgtg cgcgatggcg cgggtgccga agaccgccag ggccagcccg
      781 acggcgccgc cggccaacga cagcgtgacg ctctcggtga gcatctgccg gacaagccgc
      841 cgccggcccg cgcctaacgc ggcgcggatg gccatctcct tctggcgcgt cgttccccgc
      901 gccagaagaa ggttcgagag attggcgcac acgatgagca tcaccacgcc caccccgaac
      961 gccagcatca ggagccccga tcgtgcccgc gcgctcacgt gatcctcgag cgagcttgcg
     1021 agcggcgtga acgtgttcgt attcggattc tccgccgcga ttcggggcgt gagcaccttc
     1081 agttcggcgc gcgcgctggc gag
//
LOCUS       DASUCJ010000052         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_77008, whole genome shotgun sequence.
ACCESSION   DASUCJ010000052 DASUCJ010000000
VERSION     DASUCJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77008"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..388)
                     /locus_tag="VFU90_00585"
     CDS             complement(95..388)
                     /locus_tag="VFU90_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478290.1"
                     /translation="MTLLIGIVLLLVGALVTVGNCWTVMASIRMRKHVSMIPLLGGPV
                     AFVGCGLIPAIGWKLGSIALGVDPACGYTLLALLTMPLWSRWKRDRSRSEGPQ"
     gene            complement(385..714)
                     /locus_tag="VFU90_00590"
     CDS             complement(385..714)
                     /locus_tag="VFU90_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478291.1"
                     /translation="MVRSTTSRSVSSASSKVGALRTPNTLDVAFRIAKQESGIEKRFT
                     IHGLRYTFTDLVRRANVDAVVRRALTGHVTEEMQRHYSTVGIDEKRAAIAGVLPLVPA
                     PKVGEAG"
BASE COUNT          214 a          397 c          375 g          192 t
ORIGIN      
        1 ttcatggtcc cacctctggt cccacctgag cggtggggcc agccgcacgt agccggcgag
       61 caacccgatt cgtcgagcgt ttttctagcc acgatcactg cggaccttcc gacctactgc
      121 gatcgcgttt ccagcgagac caaagcggca tcgtcagcag cgccagtagc gtgtacccgc
      181 acgcgggatc aacaccgagc gcgatcgagc cgagcttcca gccaatcgcc ggtatcaacc
      241 cacagccgac aaacgcaaca ggtccgccga gcagcgggat catcgacacg tgcttccgca
      301 tccggatcga cgccatcacg gtccagcagt tgccaaccgt gacgagtgct ccgaccaaga
      361 gcagaacgat gccgatgagt aacgtcatcc cgcttcaccc accttcggcg ccggaaccaa
      421 aggcagcacg cccgcgatgg ccgcgcgctt ctcgtcgatc ccgaccgtgg agtagtgccg
      481 ctgcatctcc tcggtcacgt gaccggtgag cgcgcgccgc accactgcgt cgacgttcgc
      541 ccgacgtacg aggtcggtga acgtgtagcg gagcccgtgg atcgtgaacc gcttctcgat
      601 gcccgattcc tgcttggcga tccggaacgc gacgtcgagc gtgttcggcg tgcgcagcgc
      661 gccgaccttc gaggatgccg agcttaccga gcgagatgtc gtagatcgca ccacgcggag
      721 tcatcggcgg cacgtgcacg tagaccacgc ggtcgacctc gatcattccc gactgccggc
      781 agatgcacat gcgaggagcc acgtgatgcc ggtgcctccg caacgcgggc acggttggaa
      841 cgccggaacg ccgataggga cgtcgcagcc cgacgccgct tcgtcgcgcg tgagcaagac
      901 ttcgaagtct agcggctcga cccgctcggc cttcggaacg tgaagaccgg tgaagtttcg
      961 ctcgaaccga tcgcggacct cggcgaacga cggatggatc gtttcgggtg gatcgaggat
     1021 cgagatgcgc tcgcggcatc gcgcgtgctt cggccgcgag ctccgcgttg tatgagcgac
     1081 ggcgcgacgg gtccgacagc acgccgtatg ccgcgtttag atcttggaac gcgcgggtcg
     1141 cgctatcgcc cgcgacgtcc gggtggatct ggcgcgcg
//
LOCUS       DASUCJ010000053         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_321532, whole genome shotgun sequence.
ACCESSION   DASUCJ010000053 DASUCJ010000000
VERSION     DASUCJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321532"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..>1075
                     /locus_tag="VFU90_00595"
     CDS             276..>1075
                     /locus_tag="VFU90_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU5478292.1"
                     /translation="MKIAAPLCLLSGALLLAGCASDPTAMKMGSQDAKTTATGSAGGA
                     ASANANKALEHCDKPYGTLAMVEDQSATWYIQLTRDYQLTSTIPVLRLLVQQSNCFVV
                     VERGRAMANMQQERALQQSGELRAGSSFGKGQMVSADYTMTPSITFSAKNTKGAGGAL
                     AGFGGWVGAVAGVAGGFHQNEASTMLLLTDNRSGVQVGAAEGSASKVDFDVGAAVFGW
                     SGGGGLGGYTNTPQGKVIVAAFTDSYNQLVQAVRNYTPQMMGNGQGLGT"
BASE COUNT          193 a          335 c          380 g          167 t
ORIGIN      
        1 gggatcgcga aaatgccccg ccgcaaatgc agttcgcgga atgcctcacc gcgtcgttcg
       61 agctccatgc gtcctccgcc atcccggcgc gcccaggtgg tgcgccgatc tatcgcaaag
      121 acgattgtcg cagatgccga atattcacgt gtgtcgtccg acgaggtgaa ttgaccccgt
      181 cggaacgttt gaggatactc accggctgga gccggctcgc agcaaagagc gtcggccgga
      241 agatcgtcgc acgagaccga tagggggaga gctcgatgaa gatcgccgcg ccgctgtgtc
      301 tgttgtccgg ggccctgctg ctcgcaggct gcgccagtga tccgaccgcg atgaagatgg
      361 ggtcgcagga cgcgaagacg accgcgactg gctcggccgg aggcgcagcc agcgccaatg
      421 cgaacaaggc gctcgagcac tgcgacaagc cgtacgggac gctcgcgatg gtcgaggacc
      481 agagcgcgac gtggtacatc cagttgacgc gcgactatca gctcacgtcc acgatcccgg
      541 tgctgcggct cctcgtccag cagtcgaact gcttcgtcgt cgtcgagcgc gggcgcgcga
      601 tggcgaatat gcagcaggag cgcgcgctgc agcagtcggg cgagcttcgc gccggcagca
      661 gcttcggcaa agggcagatg gtgtcggcgg attacacgat gacgccgtcg atcacgttca
      721 gcgcgaagaa tacgaaaggt gcgggtgggg cgcttgcggg cttcggcggc tgggtcggcg
      781 ccgtcgccgg cgtcgcgggg ggattccacc agaacgaagc gtcgacgatg ctgcttctca
      841 ccgacaatcg ctcgggcgtg caggtcggcg ccgccgaggg cagcgcgtcg aaggtcgatt
      901 tcgacgtcgg cgccgcggtg ttcggctggt ccggcggcgg cgggctcggt ggctacacca
      961 acacgccgca gggcaaggtg atcgtcgccg cattcaccga ttcgtacaac cagctcgtgc
     1021 aggccgtgcg caactacacg ccgcagatga tgggcaacgg ccagggcctc ggcac
//
LOCUS       DASUCJ010000054         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1345610, whole genome shotgun sequence.
ACCESSION   DASUCJ010000054 DASUCJ010000000
VERSION     DASUCJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345610"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..575
                     /locus_tag="VFU90_00600"
     CDS             162..575
                     /locus_tag="VFU90_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5478293.1"
                     /translation="MHQPRHDADSYQRVEVITGRRRRREWSDAEKARILAESADPEVN
                     ISEVARRNGVSRGLLNVWRRKARIASSAGPQFVEIRLDDGVEARPDAIDPPPRVVARP
                     AERIEVMIAGAMVRVPVGVDAVTLERVLAAVRSTR"
     gene            599..928
                     /gene="tnpB"
                     /locus_tag="VFU90_00605"
     CDS             599..928
                     /gene="tnpB"
                     /locus_tag="VFU90_00605"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU5478294.1"
                     /translation="MFVATQPIDFRKGVHGLVALVAEGLAGEPYSGDVFVFRSKRSDR
                     LKLLVFDGSGLVLATKWLEDGSFAWPPVRAGTMRVTGAQLAMLIEGLSEWSQVVPKVT
                     KRPTKVG"
     gene            979..>2329
                     /locus_tag="VFU90_00610"
     CDS             979..>2329
                     /locus_tag="VFU90_00610"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEU5478295.1"
                     /translation="MAIRPEDLPSDPAALAEMVLAFDGENEDLRAQIATLKGLIFGAR
                     SERSAIICAEQLALDLGRTADAQPPANDDEPESDAPRTKRRKPRRNIGALPAHLPRVE
                     QVIEPASTLCPCCSGQMHRIGEETSEALDRVPAWLRVLRTIRPKYACRACEGPIVQAP
                     APARLVEGGMATTALIAHIAAAKYAWQSTLYRQTQILAGHGVVVDRQTLARWMGSAAW
                     MVKGLYDLQLQTMHRFERLFCDETPMPVLDPGRGRTKICQFWAHAIDDRAWKGPAPPA
                     VAYVFAGGRGKKEIVAQLADFEGVLQVDGYAAYVSLAGDAKTSGKIRLAYCLVHARRN
                     FVRVHKTTNSPFAREVIERIGAVYAIEERIRGLDAGERRAARQAETKPLMEALKARLV
                     AVKDGISRRSTLIKAIDYMLERWQGLTAFLDDGRLEPDTNTVERSIRPIAIGKKNSLF
                     "
BASE COUNT          384 a          703 c          842 g          400 t
ORIGIN      
        1 gattccaagt tcgcccagcc cgggcatgtc tggccttgac gtgctgtcga tctgcatcag
       61 ttccacttga ttgtaaccgg tacgccgccc ccacctcgcc tgcggcgttc gggtgagcga
      121 gacttgcgct tcgattcgac aatgcgacgg gcgctaattc catgcatcaa cccaggcatg
      181 atgccgactc ttatcagcgc gtcgaggtga tcaccgggcg gcgacggcgg cgcgaatgga
      241 gcgatgcgga gaaggcgcgg atcctggccg agagcgccga tccggaggtg aacatctccg
      301 aggtggcgcg gcgcaatggg gtgagccggg gattgcttaa tgtgtggcgg cgcaaggctc
      361 ggatcgcttc cagcgcagga ccgcaattcg tggagatcag gctcgatgat ggtgtcgagg
      421 cgcggccgga cgcgatcgac ccgccgcctc gcgttgtggc gcgtccggcg gaacggatcg
      481 aggtgatgat cgccggcgcg atggtgcgcg tgcccgtcgg cgtcgacgct gtgacgctgg
      541 agcgcgtgtt ggccgcagtg aggtcgacgc ggtgatcccg ttcggtcggg aagtccgggt
      601 gttcgttgcg acgcaaccga tcgactttcg aaaaggcgta cacggccttg tcgcgttggt
      661 ggcggaggga ttggcgggcg agccctacag cggggatgtt ttcgttttcc gctcgaagcg
      721 atcggaccgt ttgaagcttc ttgttttcga cggctcgggt ctcgttctgg cgaccaaatg
      781 gctcgaggac gggagcttcg cctggccgcc ggttcgcgcg gggacgatgc gggtgacggg
      841 ggcgcaactg gcgatgctga tcgaaggtct ctcggagtgg tcgcaggtgg tcccgaaggt
      901 gacgaagcgg ccgacgaaag tgggctgagt cgccttgttt tgctggcgac tccgagcgtt
      961 ttcgtgtagc tctccgacat ggcgattcgc cctgaagatc tgccctcgga ccctgcggct
     1021 ctcgccgaga tggtactggc tttcgacggc gagaacgagg atctgcgcgc gcagatcgcc
     1081 acgttgaagg gcctgatctt cggcgcgcga tcggagcgct cggcgatcat ctgcgccgaa
     1141 cagctcgcgt tggatctggg acggaccgcc gacgcacagc cgccggccaa cgacgacgaa
     1201 ccggagtcgg acgcgccgag aacgaagcgg cgcaagccac ggcgcaacat cggcgcgctg
     1261 ccggcgcatc tgccccgagt cgaacaagtg atcgagccgg cgtccacgct ctgcccgtgc
     1321 tgctcgggtc agatgcatcg gatcggcgag gagaccagcg aagcgctcga tcgggttccc
     1381 gcgtggctgc gcgtactgcg cacgatccgt cccaagtacg cctgccgcgc ctgcgagggt
     1441 ccgatcgtcc aggcccctgc gccggcgcgg ctcgtcgagg gcggcatggc gacgacggcg
     1501 ctgatcgcgc acatcgccgc ggccaaatac gcctggcagt cgacgctcta tcgtcagacg
     1561 cagatcctgg cgggccacgg cgtcgtcgtc gaccgtcaga cgctggcgcg ctggatgggg
     1621 agcgcggcgt ggatggtcaa gggcctctac gatctgcaac tgcagaccat gcatcgcttt
     1681 gagcggctgt tctgcgacga gacgccgatg ccggtgctcg acccgggacg cggccgcacg
     1741 aagatctgcc agttctgggc gcacgccatc gacgatcggg cctggaaggg tccggcgccg
     1801 ccggcggtcg cctacgtgtt cgcgggcggt cgcggcaaga aggagatcgt ggcgcagttg
     1861 gccgacttcg aaggcgtgct gcaggtcgac ggctatgccg cctacgtctc gcttgcgggc
     1921 gatgcgaaga cgtcgggcaa gatccggctg gcgtattgtc tcgttcacgc gcgccgcaac
     1981 ttcgtgaggg tgcacaagac gacgaactcg cccttcgcca gggaggtcat cgagcgcatc
     2041 ggcgccgtct acgcgatcga ggagaggatc cgcggcctcg acgccgggga acgccgcgcg
     2101 gcgcggcagg ccgagacgaa gccgctgatg gaggcgttga aggcccgtct ggtagcggtg
     2161 aaggacggga tctcccggcg gtcgacgctc atcaaggcga tcgactacat gctcgaacgc
     2221 tggcagggtc tgacggcgtt cctggacgac gggcggctcg agccggacac gaacacggtc
     2281 gagcgatcga tcaggccgat tgcaatcgga aaaaagaata gcttgttca
//
LOCUS       DASUCJ010000055         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_245205, whole genome shotgun sequence.
ACCESSION   DASUCJ010000055 DASUCJ010000000
VERSION     DASUCJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245205"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..>1102)
                     /locus_tag="VFU90_00615"
     CDS             complement(184..>1102)
                     /locus_tag="VFU90_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478296.1"
                     /translation="VWLMGAVALASFSIARVGRAQTPGSTEPEPPLPAPLAASFALRT
                     PAASTGVRAETAYGLDSGASSTIVQYLSASYAPIDRLSFFVRGGWVDYAPNTGPSGTA
                     LTNVALGGQWADKLSREWRVAGAIGTGLPVGQGGGDLPDPGAAAAIAAGNIARSRLEG
                     STMFSPNDLAPFVGGDIAWVSHGFTVQAEANVFQLFRVRGTKVDPDATKTSFMGGVHG
                     AYFVIPELSIGLEVRDNTFLTTPAAVSAGKTSRTWVTAGGGVRAHLQLGPHVWFRPGL
                     AYFQPLNDPTPTISASSYHVFQLDLPLTF"
BASE COUNT          194 a          358 c          395 g          155 t
ORIGIN      
        1 tgaggagcgt tggcgggcgg cgcttcttcg catgcgcaga gcagcaagaa gacgatgacg
       61 gctgctgcgc ttccggcgag cacgcgcccg agcgagtcgc gcaaccgaac acgggagctt
      121 tccatgaacg caagcgtgcg cgcctctgcg cacgctcgcc ataaggcgaa caccctagcg
      181 acgtcagaaa gtcagcggca ggtcgagttg gaatacgtga taactgctcg ccgagatcgt
      241 cggggtgggg tcgttgagcg gttggaagta ggcgagcccc ggacggaacc aaacgtgggg
      301 accgagctgc aggtgcgcgc gcacgccgcc gccagcggtc acccaggtgc gtgacgtctt
      361 gcctgccgac acggcggcgg gcgtcgtgag gaacgtgttg tcgcgcacct ccaggccgat
      421 cgagagctcg gggatgacga aatacgcacc gtggacgcct cccatgaagc tcgttttcgt
      481 tgcgtcggga tcgaccttgg tcccgcgcac gcgaaagagc tggaatacgt tggcctcggc
      541 ctggacggtg aagccgtgcg agacccacgc gatgtcgccg ccgacgaagg gtgccagatc
      601 gttcggcgaa aacatggtgg acccctcgag ccgcgatcgc gcgatattgc cggccgcgat
      661 ggcggcggcg gcgcctggat cgggcaagtc gccgccgccc tggcccaccg gcaagcccgt
      721 accgatcgct ccggccacac gccattctcg cgagagcttg tcggcccact ggccaccgag
      781 cgcgacgttc gtgagggccg tgcccgacgg accggtgttc ggcgcgtagt cgacccagcc
      841 gcctcgcacg aagaacgaga ggcggtcgat cggcgcgtag gaggctgaaa ggtactgcac
      901 gatggtgctc gacgccccgg agtcgagacc gtaggcggtc tccgcacgaa cgcccgtgct
      961 ggccgccggc gtgcggagcg cgaacgacgc cgcaagcggc gcaggcagtg gtggctccgg
     1021 ttcagtcgat cccggggtct gcgcgcggcc gacgcgtgcg atcgagaacg acgcgagtgc
     1081 caccgctccc atgagccaca cc
//
LOCUS       DASUCJ010000056         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_764905, whole genome shotgun sequence.
ACCESSION   DASUCJ010000056 DASUCJ010000000
VERSION     DASUCJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764905"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFU90_00620"
     CDS             complement(<1..508)
                     /locus_tag="VFU90_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478297.1"
                     /translation="MSLKWRIAIWYATLLIVVLIVTSAIVVWRFQGILYDQSKASVSA
                     TMRAIVQATRQTNPFSLENSSGSLQFLFNSSNLANWNSATSYVQVDSGDGYPLAKTAN
                     LGDSTIPPNPAVSAKHDTAFRNVSIDGRPFLVEDRFLREGDVSAIVHVAEPLDAVNLT
                     FRRARDAIV"
     gene            complement(518..1210)
                     /locus_tag="VFU90_00625"
     CDS             complement(518..1210)
                     /locus_tag="VFU90_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5478298.1"
                     /translation="MEKPAAHQQQPYRILVIEDDAAISRVLQLELEHEHYDVDVARDG
                     LTGLEKALKEPDLVILDLMIPRMDGFEVCKRIRAKSHVPIIMLTAKDRVPDRVAGLDL
                     GADDYVTKPFSTPELLARVRARLRERSPQSNVIEYRDVVMDRDRHEVHRGGKAVSLTA
                     KEYALLEYLLLHRNKVHTRDELFNGVWGSDFLGDSNLIDVYVRYLRGKIDDGFDDKLI
                     TTVRGVGYTIKD"
     gene            complement(1219..>1504)
                     /locus_tag="VFU90_00630"
     CDS             complement(1219..>1504)
                     /locus_tag="VFU90_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478299.1"
                     /translation="AYRPAPPFAVWEVAGLGALAAVTVWLGILIAIGGGGLFLSTVSA
                     IAVAIGESLSDVRVLAWLAAGAATALWLTFFTGSQPFAVLAARFGRKTSR"
BASE COUNT          263 a          466 c          506 g          269 t
ORIGIN      
        1 gcacgatcgc gtcgcgcgcg cgccggaacg tcagattgac ggcgtcgagc ggttcggcaa
       61 cgtggacgat cgccgaaacg tcgccttcgc gcaggaagcg gtcctcgacc agaaacggcc
      121 gcccgtcgat cgagacgttg cggaacgcgg tgtcgtgctt cgcggaaacc gccggattcg
      181 gcggaatcgt cgagtcgccg aggttggcgg ttttcgccag cggatagccg tcgcccgaat
      241 ccacctgcac gtagctggtg gccgagttcc agttcgcgag gttgctgcta ttgaagagaa
      301 actgcaggct gccggatgag ttttccaacg agaacgggtt ggtttggcgg gtcgcctgca
      361 cgatcgcgcg catcgtcgcg ctgacgctcg ccttggactg atcgtagaga atcccctgaa
      421 agcgccagac gacgatcgcg ctggtgacga tcagcacgac gatcagcagc gtcgcgtacc
      481 agatcgcgat ccgccacttt agcgacacgg gccggggtca gtccttgatc gtgtagccga
      541 cgccgcggac ggtcgttatg agcttgtcgt cgaagccgtc gtcgattttg ccgcgcaggt
      601 agcgtacgta gacgtcgatc aagttagaat cgcccaggaa atcgcttccc cagaccccgt
      661 tgaacagctc gtcgcgcgta tgcaccttgt tgcggtgcag cagcaggtat tccagcaggg
      721 cgtactcttt ggcggtaagc gagaccgcct tgccgccccg atgcacttcg tgccggtcgc
      781 gatccatcac gacgtcgcgg tactcgatga cgttggactg cggcgaccgt tcgcgcagcc
      841 gcgcccggac ccgcgcgagc agctccggcg tggaaaacgg cttggtgacg tagtcgtcgg
      901 cgccgaggtc gagcccggcc acccggtcgg gcacgcgatc cttggcggtg agcatgatga
      961 tcggcacgtg gctcttggcg cggatgcgct tgcagacttc gaagccgtcc atccgcggga
     1021 tcatcaaatc gaggattacg agatcgggct ctttgagcgc cttctcgagg cccgtcaacc
     1081 cgtcgcgtgc gacgtcgacg tcgtagtgct cgtgctcgag ttcgagctgg agcacgcggc
     1141 tgatggcggc gtcgtcctcg atgacgagaa tgcggtaggg ttgttgctgg tgtgctgccg
     1201 gtttctccat gaaccggttt atcgactagt cttccgcccg aacctcgcag ccagcactgc
     1261 aaagggttgc gatccggtaa aaaacgtcag ccaaagcgcg gtcgcggcac cggccgccag
     1321 ccacgcgagc acgcgtacgt cggagagcga ttcgccgatc gcgacggcga ttgcggagac
     1381 ggtgctcagg aacagtccgc cgcccccgat cgcgatcagg atgcccagcc agacggtgac
     1441 ggccgccagc gcgccgagcc cggcgacttc ccagacggca aacggcggtg cggggcggta
     1501 ggcg
//
LOCUS       DASUCJ010000057         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_810627, whole genome shotgun sequence.
ACCESSION   DASUCJ010000057 DASUCJ010000000
VERSION     DASUCJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810627"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..>1037
                     /locus_tag="VFU90_00635"
     CDS             174..>1037
                     /locus_tag="VFU90_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5478300.1"
                     /translation="MSRAYYVTTPIYYISGNPHIGHASTTVGADVLARTARTAGPARF
                     LTGTDEHGQKVANAAVEAGKTPIEWCDDLVPRWKALFAAYHVAYDDFIRTTEPRHERV
                     VAAVFERMRANGDVYLGKYEGWYCVYDETFWPESKLAGGRCPTCGREVGRISEDDWFF
                     RLSKYRDRLLEHFRTHPQWVRPKSVYNEMMALLEEGLDDLSITRTNLEWGIPVPGGGG
                     VIYVWFDALLNYISAIGWSTDPATFSTYWPASAQLIGKEIARFHTLIWPAMLWSIGES
                     APELVFAHGWIT"
BASE COUNT          182 a          327 c          354 g          174 t
ORIGIN      
        1 ggcgatctcg atgcacgaat cgacgccctg ctggcagggg actgccgcgt cggcgaaatc
       61 gcgaagcggc ttgcggccgc cggattgggc gaacgcggcg aactctacgg gcgtgcgagc
      121 cggcgcaaag cggccggcaa gggagcggcg gcgcccgccg aaaggctccg ccgatgagcc
      181 gcgcctacta cgtcaccacg ccgatctact atattagcgg caatccgcac atcggccatg
      241 cgtccacgac cgtcggtgcc gacgtcctgg cgcgcacggc gcggaccgcc ggtccggcgc
      301 ggtttctgac cggcaccgac gaacacgggc aaaaagtcgc caacgccgcc gtcgaggccg
      361 ggaagacgcc gatcgaatgg tgcgacgatt tggtgccgcg ctggaaggcg ctgttcgccg
      421 cgtaccacgt cgcctacgac gacttcatcc gcaccacgga acctcgccac gagcgcgtcg
      481 tggcggcggt cttcgagcgc atgcgtgcga acggcgacgt gtatctgggc aagtacgagg
      541 gctggtattg cgtctacgac gagacgtttt ggccggaatc caaactcgcc ggcggacgat
      601 gcccgacgtg cggacgcgaa gtaggacgca tttcggaaga cgactggttc ttccggttgt
      661 cgaagtatcg cgaccgtctg ctggagcact ttcgtacgca tccgcagtgg gtgcgtccca
      721 agagcgttta caacgagatg atggcgctgc tcgaggaggg gctcgacgac ctctcgatca
      781 cgcgcacgaa tctggagtgg ggaatcccgg ttccgggcgg cggcggcgtg atttacgtgt
      841 ggttcgacgc gctactcaac tatatctcgg cgatcggctg gtcgaccgac ccggcaacct
      901 tctcgacgta ctggccggcg tcggcgcagt tgatcggcaa agagatcgcg cgctttcaca
      961 ccctgatctg gccggcgatg ctgtggtcga tcggcgaatc ggcgcccgag ctggttttcg
     1021 cccacggctg gatcacg
//
LOCUS       DASUCJ010000058         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_46581, whole genome shotgun sequence.
ACCESSION   DASUCJ010000058 DASUCJ010000000
VERSION     DASUCJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46581"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFU90_00640"
     CDS             complement(<1..444)
                     /locus_tag="VFU90_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5478301.1"
                     /translation="MYRKTVLPSGLTVLTEAMPAVRSASVGVWADVGSSVESHDKRGV
                     SHIVEHMLFKGTTSRTAREIAETMDGVGGNLNAFTDKESTCYYAKVIDRHLPLATNVL
                     ADMFLHSTFDPQELGKEQKVILEEIKMYDDSPDELIHDLFLQTMWN"
     gene            complement(463..1005)
                     /locus_tag="VFU90_00645"
     CDS             complement(463..1005)
                     /locus_tag="VFU90_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478302.1"
                     /translation="MAATAGLAGALAANGGAWASAPSIADLDAAARASGNRTDVATHV
                     GESVFGQLWPAEVSQISANEVGKHLIVGVRIWGVKFHRPVSREDFVDEVVAVIGKVFA
                     AAPEAEEVDVWASVPLQVGKNVVVSGDLAMPTVRTVFTLAAYRDESPDRIAARARATN
                     SPDVFWDEEWVTTAFKKPGT"
     gene            complement(1010..>1554)
                     /locus_tag="VFU90_00650"
     CDS             complement(1010..>1554)
                     /locus_tag="VFU90_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5478303.1"
                     /translation="RAIAVCRQAAAELENAGFRVRVDARDQQPGWKYGEWDVRGVPVR
                     IDVGPRDVEAGTAVLVRRDRAKGDPQQKASVSLEALARTLRATLDDVEKSLYAQAAEF
                     LRTHTVSAGSRDEFFAMCTDRAGMIDIPWCRRPECEARVKAETGATTRNLRPLAEGAP
                     VLCVACGEPAVVSAYFAQSY"
BASE COUNT          255 a          545 c          473 g          281 t
ORIGIN      
        1 gttccacatc gtctggagaa agagatcgtg gatcagctcg tcgggcgagt cgtcgtacat
       61 cttgatctct tccagaatga ccttctgctc tttcccgagc tcctgcgggt cgaacgtcga
      121 gtgcaggaac atatcggcga gcacgttggt cgccaacggt aagtggcggt cgatcacctt
      181 ggcgtaatag caggtgctct ctttgtccgt aaacgcgttc aggttgccgc ccacgccgtc
      241 catcgtttcg gcgatctcgc gcgcggtccg gctcgtcgtt cccttgaaca gcatgtgctc
      301 gacgatgtgc gagacgccgc gcttgtcgtg cgactccacc gaggatccga cgtcggccca
      361 caccccgacg ctggcagacc gcacggcggg catggcttcg gtcaataccg tcaggccgct
      421 gggaaggacc gtcttgcgat acatgattgc ggggcgagct ccttaggttc ccggtttctt
      481 gaaggcggta gttacccact cttcgtccca gaacacgtcg ggagagttgg tcgcgcgagc
      541 gcgagccgca atccggtcgg gcgattcgtc gcgataggct gccaaagtga ataccgtgcg
      601 aaccgtcggc atcgcgagat ctccgctcac gacgacgttt ttccccacct gaagcggaac
      661 gctcgcccaa acgtcgactt cttccgcctc cggcgcggcg gcgaaaactt tcccgatcac
      721 cgccacgact tcgtcgacga agtcctcccg gctgaccggt cggtgaaact tcacgcccca
      781 aatgcgcacg ccgacgatca ggtgcttacc gacttcgttg gcggaaattt ggctaacttc
      841 ggccggccac agctgcccga agacgctctc gccgacgtgc gtcgccacgt cggtccggtt
      901 ccccgaggca cgcgcggcgg catcgagatc cgcaatcgag ggtgccgacg cccacgcgcc
      961 gccgttggcc gcgagcgcgc cggcgagccc ggcagtcgcc gccaaggctc taatacgatt
     1021 gcgcaaagta cgcactcacg accgcgggtt cgccgcacgc cacgcagagg accggcgccc
     1081 cctcggccag cggccggagg ttacgggtcg tcgcgccggt ttcggccttt acgcgcgcct
     1141 cgcattcggg gcgccggcac cacggaatat cgatcattcc ggcgcgatcg gtgcacattg
     1201 cgaaaaactc gtcgcggctg cccgcgctca ccgtatgggt tcgcagaaac tcggcagcct
     1261 gagcgtagag cgatttctcg acgtcgtcca gcgtcgcacg cagcgtgcgc gccagcgcct
     1321 cgagcgacac gctcgctttt tgctgcggat cgcccttggc gcgatcgcgg cgaaccagca
     1381 cggccgtccc ggcctcgacg tcgcgcggac cgacgtcgat cctcaccgga acgccgcgca
     1441 cgtcccattc gccgtacttc caccccggct gctgatcgcg cgcgtcgact cgcacgcgga
     1501 agccggcatt ttccagctcc gccgccgcct gccggcacac ggcgatcgcc cgat
//
LOCUS       DASUCJ010000059         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1376254, whole genome shotgun sequence.
ACCESSION   DASUCJ010000059 DASUCJ010000000
VERSION     DASUCJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376254"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..725
                     /locus_tag="VFU90_00655"
                     /pseudo
     CDS             42..725
                     /locus_tag="VFU90_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(773..>2096)
                     /locus_tag="VFU90_00660"
     CDS             complement(773..>2096)
                     /locus_tag="VFU90_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5478304.1"
                     /translation="LIFLALRWPLDGLFGFYVLLVPFDNLLSTGSFGTITKYLGLIAG
                     AFLLLALVRRRRISFASSPLRILCVLFLWMFASTLWAIDQKAALAMLPTYGGLMLLYG
                     VLSMIPLSPRQFGVLLFLVAAGGICAAAYGAHAFYSDPSFSQESLVMRRLVVQIGDNF
                     IDPNHFSDALLFPVAIVTMWALRTRRLLVRLGSVAALALLVVAIMFSGSREGLTALVV
                     IAAYYLWRSRYRVRLAIAIASVAVVAASVQSSVFARFSSALETGGSGRTSIWAVALEA
                     AKHRLFQGYGIGNFTEAFNLFYLDVHQPYPYGWNSPAHNLIVHYIVELGLVGVILIGA
                     FLWAQFRSLRDVEPDSQLYDYRIVMEAVLLAIVTVSMTIDLFQYKYAWLAFAMAALLR
                     NAALADQQSAAMRTAKSAMMPERSARSAMRALPRSPRSRNTRLSTSES"
BASE COUNT          415 a          711 c          673 g          297 t
ORIGIN      
        1 tctatcttgc cggcacgcgc acgcgctacg acgtggtcgt gatgagctcg ccgccgatca
       61 cggtggcgtt tcccgcgttg gtgggagcgg cccggcaccg agcgcgtttg gtcgtcgacg
      121 tccgggacgt tttccccgat atcgccgtcc agatgggcgt gtggaaatcg ggcagcctca
      181 tcgtccgcct ggtcgagcgg ctcgttcgcc gcctgtatcg ccgcgccgat ctcgtcgtcg
      241 tcgtgacgcc cacggcgctc gcgcagatcg cccagcgcgg cgtcccgccc gaacggctgg
      301 tgctcgcgcg caacgccgcc gagcggatcg cgctgacgct cgagccggcg ccgcgcgacg
      361 gaacgttcac cgccgtctat gcgggcaatc tcgggctcac caccgacgtc gacgtgctgg
      421 tcgacgccgc aaaactcgtc gccaaagacg gcgtaacgat agaaatcgtc ggcgacggcg
      481 cgcagcgcac gcacctgcgc gaacggctcg cgcgcgagaa cgtcgccaac gtccgcctga
      541 ccggcagcct gccgcgccag gaagcgatgg ccagagtagc gagcgccgac gtagcggtca
      601 tcccgctgcg caaaggcatc gaggagagca tcccgaccaa gctgtacgac gcgctctcgg
      661 tcgcctgccc ggtgatcgtg gccgccggcg gcgaagcccg gaacgaagga acgtcgctcg
      721 gcgcgatctg cacgccgccc ggcgacgcgc acgcgttggc cgccgagctg cgtcagcttt
      781 cgctggtcga taaacgggtg ttgcgcgatc tcggcgaccg gggcaaagcg cgcatcgccg
      841 atcgcgcgga ccgttccggc atcatggccg atttggccgt gcgcatcgcg gcgctctgct
      901 gatccgccag cgccgcgttg cgcagcagcg ccgccatcgc aaacgctagc cacgcgtact
      961 tgtactgaaa aagatcgatc gtcatcgaca cggtgacgat tgccagcagg acggcctcca
     1021 tcacgattcg gtagtcgtac agctggctgt ccggctccac gtcgcgaagc gaacgaaact
     1081 gcgcccacaa gaacgcgccg atcaagatca cgcccacgag cccgagctcg actatgtagt
     1141 gcacgatcaa attatgagcc gggctgttcc agccgtacgg atacggctga tgaacgtcga
     1201 ggtagaacag gttgaaagcc tcggtaaagt tcccgattcc gtagccttgg aaaagtcggt
     1261 gtttcgcggc ctccaacgcg accgcccaaa tcgacgttcg ccccgatccg cccgtctcga
     1321 gagcgctcga gaaccgtgcg aacaccgagg actgcacgct cgccgcgacg accgccaccg
     1381 aggcgatcgc gatggcgagc cgcacgcggt agcgcgagcg ccagagatag tacgccgcga
     1441 tgacgaccag cgccgtcaat ccctcacgcg atccgctgaa catgatcgcg accaccagca
     1501 gcgccagcgc cgcaacgctg ccgagccgaa cgagcagccg gcgcgtgcgc agcgcccaca
     1561 tcgtaacgat cgcgacgggg aagagcagcg cgtccgaaaa gtggtttggg tcgatgaagt
     1621 tgtcgccgat ctggacgacc agccggcgca tcaccaagct ctcttgcgag aacgacggat
     1681 cactgtagaa ggcgtgggcg ccgtacgccg ccgcgcaaat tccgccggcg gccaccagaa
     1741 agagcaggac cccaaactgc cgcggggaga gcgggatcat gctgagcacc ccatacagca
     1801 gcatcagtcc gccgtaggtg ggcaacatcg caagcgccgc tttttggtcg atcgcccaga
     1861 gcgtgctcgc aaacatccac agaaaaagca cgcacaggat ccgcaacgga ctgctcgcga
     1921 acgaaattcg gcggcgccgc acgagggcga gcagcaagaa cgcgcccgcg atcaggccga
     1981 gatacttggt gatcgttccg aacgagccgg tgctgagcag attgtcgaac ggcaccagca
     2041 gcacgtagaa tccgaacagg ccgtcgagcg gccagcgcag cgccagaaag atcagg
//
LOCUS       DASUCJ010000060         1473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1467933, whole genome shotgun sequence.
ACCESSION   DASUCJ010000060 DASUCJ010000000
VERSION     DASUCJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467933"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="der"
                     /locus_tag="VFU90_00665"
     CDS             complement(<1..939)
                     /gene="der"
                     /locus_tag="VFU90_00665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5478305.1"
                     /translation="MRTVEPAGDGNALRPATVAVVGRPNVGKSALFNRLIGQRLAIVE
                     DTPGVTRDRLYALCDWRGRVFSLVDTAGIDPDARSEQSNGFAEAARRQAEAAAAESDV
                     VVLVVDAQTGLHPLDEDVAAILRRKRRPIVLVANKAESPSAAASVHAEFSALGFGEPV
                     AVSAIHGEGTGDLLDRITDLLPPEAAPGTGESELSLAIVGRPNVGKSSLLNALIGEER
                     AIVSEVPGTTRDAIDTILAWRGRSIRLIDTAGVRKKPEAHGAIEYYAALRSLRAISRC
                     DIALLVFDAMSGVTTQDRRLAGIAIEERKGLILVGNK"
     gene            1025..>1473
                     /locus_tag="VFU90_00670"
     CDS             1025..>1473
                     /locus_tag="VFU90_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478306.1"
                     /translation="MASIYIETFGCQMNEADSQYVAERASSAGYAIAQRPEEANVLVL
                     NTCTVRDNAERRAYGRMNHFKMLKDADPAVRLVVMGCLAEQDRDRMQTLAPHVDAVFG
                     TKELVQLGDQLAAWQPELADEIDFTQERALLMPLGGTADAVAGPFSA"
BASE COUNT          250 a          496 c          494 g          233 t
ORIGIN      
        1 cttgtttccg accagaatga gacccttgcg ctcttcgatt gcgatccccg ccaggcggcg
       61 gtcttgcgtc gtcacgccgg acatcgcgtc gaacacgagc agcgcgatgt cgcagcgcga
      121 gatcgcccgg agcgagcgca aggcggcgta gtactcgatc gcgccgtgcg cttcgggctt
      181 cttgcgcacg ccggcggtgt cgatcaaacg aatcgagcgg ccgcgccatg cgaggatcgt
      241 gtcgatcgcg tcgcgcgtcg ttcccggaac ctccgaaacg atcgcgcgct cctcgccgat
      301 cagcgcgttg agcagcgagc tcttcccgac gttggggcgt ccgacgatgg cgagcgagag
      361 ttcgctttca ccagtgcccg gcgccgcctc gggcggcagc aggtcggtaa tgcgatcgag
      421 cagatcgccg gtcccttcgc cgtgaatagc ggagacggcg accggctccc caaagccgag
      481 cgccgaaaac tcggcgtgga ccgaagcggc ggccgacggg gattccgcct tattggcaac
      541 cagcacgatc ggccggcgtt tccgccggag aatcgccgcg acgtcttcgt cgagaggatg
      601 aagcccggtt tgcgcgtcga ccaccaagac gaccacgtcg gattcggccg cggcggcctc
      661 cgcctgacgg cgcgccgctt cggcaaagcc gttgctctgc tccgagcgcg cgtcgggatc
      721 gatgccggcc gtatcgacga ggctgaagac gcgtccccgc caatcgcaca gcgcgtacag
      781 ccggtcgcgg gtaacgccag gggtgtcctc cacgatggcc agccgctgcc cgatcagccg
      841 gttgaagagc gcgctcttgc cgacgttcgg acgcccgacc actgcaaccg ttgccgggcg
      901 cagggcgttt ccgtcaccgg caggttctac tgtgcgcacg ccgccttacg ttcgtagcgc
      961 ggttgcgcga cacccatcga gccggaagag ccggccggcg gcacgaacag ccagcggcaa
     1021 cctcatggcg agcatctaca tcgagacctt cggatgtcaa atgaacgagg cggattcgca
     1081 gtacgtggcg gagcgcgcgt cctcggccgg ctacgccatt gcccagcggc ccgaagaggc
     1141 gaacgtactg gtgctcaaca cctgcaccgt tcgtgacaac gcggagcggc gcgcgtacgg
     1201 ccggatgaat cacttcaaga tgctcaaaga cgccgatccc gcggtgcggc tggtggtgat
     1261 gggctgcttg gccgaacaag accgcgatcg tatgcagacg ctcgcaccgc acgtcgacgc
     1321 agtcttcggc accaaggagc tcgtgcagct cggcgatcag ttggcagcct ggcagccgga
     1381 actcgccgac gagatcgatt tcacgcagga gcgcgcgctg ctgatgccgc tgggcggaac
     1441 ggcggatgcc gtcgccggtc cgttctcggc ctt
//
LOCUS       DASUCJ010000061         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_459249, whole genome shotgun sequence.
ACCESSION   DASUCJ010000061 DASUCJ010000000
VERSION     DASUCJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459249"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFU90_00675"
     CDS             <1..949
                     /locus_tag="VFU90_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478307.1"
                     /translation="VAPNATIDVEDVNANVTMPGELGDVRPGDFARVEMRKDGRVTRI
                     VDEFGSRSGKIVAVATGQFVLDSGQVVTAGRSTEVSLNGKGAGFVDLRPGDAVTVRYN
                     VETNEVREVLASRTLPAAAASGAGTLQVSSNVNGTLQPNDNVDVTLHGPAGGSATFDI
                     GSYVTNLAMHESSPGTYAGSYRIPRSANFDEAPIVGRLVLAGGTTLEGQAAQTISASS
                     IPPGIVDFAPDNGSTVNNDRPAVYATFAADGLPVNPASASISINGRDVTPECVRTPQF
                     IQYLPQYAYPDGPMRVVVRVADRAGNATTKSWTFTIRTH"
     gene            970..1317
                     /locus_tag="VFU90_00680"
     CDS             970..1317
                     /locus_tag="VFU90_00680"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU5478308.1"
                     /translation="MDDSCLFCRIVRGEIPAKEIHRDDHVVAIEDVNPQAPAHALVLP
                     LRHLDDVAALANPENAALAHALMTAAARLGSELGGGRGFRLVVNTGPDGGQTVGHLHV
                     HVLAGRPMRWPPG"
     gene            1332..1892
                     /locus_tag="VFU90_00685"
     CDS             1332..1892
                     /locus_tag="VFU90_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5478309.1"
                     /translation="MPELWTIGHGTVPLAEFVAYLERARIARLVDVRRFPSSRRNPQF
                     AAAELERALTAAGIAYERAETLGGRRRAPADSPNRGLRNDGFRGYADWMGTDAFRRAF
                     AELTAAAARERTAVMCAETPWWKCHRRLIADAAVLLGGFSVTHLIKDKEIPHALTGGV
                     IVDAGGRLAYPPPAEEFDPASPPVLP"
     gene            1967..2137
                     /gene="rpsU"
                     /locus_tag="VFU90_00690"
     CDS             1967..2137
                     /gene="rpsU"
                     /locus_tag="VFU90_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5478310.1"
                     /translation="MEVRVAPGESIESALRRFKKATQKAGILAEARKHEHYEKPSVRR
                     KKKSAAARKRRS"
     gene            2143..>2541
                     /locus_tag="VFU90_00695"
     CDS             2143..>2541
                     /locus_tag="VFU90_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU5478311.1"
                     /translation="MGSTRDRIAADMKDAMKARDQTRLDTLRSAISGFTYKRTEAGKE
                     LSDSDELDVLRRLVKQRGDSLAEFEKAGRVDLADRERAEREILRAYLPRQKSAAEIRE
                     TVANVLAGLAPEARNPGAVMKAVMPQLKGEA"
BASE COUNT          457 a          826 c          873 g          385 t
ORIGIN      
        1 ggtggcgccg aatgcgacga tcgacgtcga ggacgtgaac gccaacgtga cgatgcccgg
       61 cgaactgggc gacgtccggc cgggcgactt cgcccgcgtc gagatgcgaa aagacgggcg
      121 cgtgacgcga atcgtcgacg agttcgggtc ccgcagcggg aagatcgtag cggtggcaac
      181 cggtcagttc gtgctcgaca gcgggcaagt ggtaaccgcg ggccgcagca cggaagtgtc
      241 gctcaacggc aagggagccg ggttcgtcga cttgcgcccg ggcgacgccg taaccgttcg
      301 ctataacgtc gaaaccaacg aagtgcggga ggtgctcgcc agccggacgc tgccggccgc
      361 ggccgcatcc ggcgccggga cgctgcaggt cagctccaac gtgaacggta cgctgcagcc
      421 caacgataac gtcgacgtca cgttgcacgg accggcgggc ggctcggcaa ccttcgacat
      481 cggctcctac gtcaccaacc tcgcgatgca cgagagttcg ccgggcacct acgcggggag
      541 ttatcgaatt ccgcgcagcg ccaatttcga cgaagcgccg atcgtcggac ggctcgtgct
      601 cgccggcggg acgacgctgg aagggcaagc ggcgcagacg atttcggcct cgagcattcc
      661 gccggggatc gtcgacttcg cacccgacaa cggatcgacg gtaaacaacg atcgcccggc
      721 ggtctacgcg acgttcgctg ccgacggcct gccggtcaat ccggcgagcg cttcgatttc
      781 gatcaacggc cgggacgtga cgccggaatg cgtgcggacc ccgcagttca ttcaatacct
      841 gccgcagtat gcgtatcccg acggcccgat gcgcgtggtc gtgcgcgtcg ccgaccgggc
      901 ggggaacgcg accaccaaga gctggacgtt taccatcagg acgcactaag gtccttaagg
      961 agcggaggcg tggacgacag ctgtttgttt tgccgaatcg tacgcggcga gattcctgcc
     1021 aaagaaatcc atcgcgacga tcacgtggtc gcgattgaag acgtcaaccc gcaagcaccc
     1081 gcgcacgccc tcgtactgcc gctgcgccac ttggacgacg tcgccgcgct cgcaaatccc
     1141 gaaaacgccg cgctggcgca cgcgctgatg accgcggccg cacggctggg atccgaactc
     1201 ggcggaggac gcgggtttcg tctggtcgtc aacaccggcc cggacggcgg acagaccgtc
     1261 ggacacttgc acgtccacgt gctggcgggg cgcccgatgc ggtggcctcc ggggtagcgg
     1321 gttttcgagg cttgcccgag ctgtggacca tcggtcacgg aacggtgccg ctcgcggaat
     1381 tcgtcgcgta tctcgaacga gcgcgcatcg cacggctggt cgacgtgcgc cgttttccct
     1441 cgagccgtcg caacccgcag ttcgcagccg cggagctgga gcgggcgttg acggccgccg
     1501 ggatcgcgta cgagcgtgcc gagacgctgg gcgggcggcg gcgtgcgccg gcggattcgc
     1561 ccaaccgcgg actccgcaac gacggctttc ggggttacgc cgactggatg gggaccgacg
     1621 catttcgccg ggctttcgcc gagctgacgg ccgccgcggc gcgcgagcga accgcggtca
     1681 tgtgcgcgga aacgccgtgg tggaagtgtc accgccggct catcgccgac gccgccgtcc
     1741 tgctgggcgg cttctcggtg acccacctga taaaggataa agagatcccg cacgcgctga
     1801 ccggaggcgt catcgtcgac gccggcggcc ggctcgcgta tcctcccccc gccgaagaat
     1861 ttgaccctgc ttcgccgcct gtgttaccgt aggggctcga agacccttcg gggtctaatt
     1921 tttcgtgcgt ccgtccggac cacgccgaaa ggggggtgtt cgttacatgg aagtccgcgt
     1981 tgctccgggt gaatcgatcg agagcgcgct gcgccgtttc aagaaagcca cccaaaaggc
     2041 ggggatactt gccgaggcgc gcaagcacga gcattacgag aagcccagcg tccgccgcaa
     2101 gaagaagtcg gccgcagccc gcaagcgccg cagctaggcc gcatgggctc tacgcgcgac
     2161 cgcatcgccg cggatatgaa ggacgcgatg aaggcgcgcg accaaacgcg cctcgatacg
     2221 ctgcgttcgg cgatttccgg tttcacctac aaacgcaccg aagcggggaa ggaactctcc
     2281 gactcggacg agttggacgt gctgcgccgg ctggtcaaac aacggggcga ttcgcttgcc
     2341 gaattcgaaa aggccgggcg ggtcgatttg gccgaccgcg agcgcgcgga acgcgagatc
     2401 ctgcgtgcct atttgccgcg ccagaaatcg gccgccgaga ttcgcgagac ggttgcgaac
     2461 gtgctggccg gactcgctcc cgaggcgcgc aatccgggcg ccgtaatgaa ggccgtgatg
     2521 ccgcagctca agggcgaggc c
//
LOCUS       DASUCJ010000062         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_734346, whole genome shotgun sequence.
ACCESSION   DASUCJ010000062 DASUCJ010000000
VERSION     DASUCJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734346"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2825
                     /locus_tag="VFU90_00700"
     CDS             <1..>2825
                     /locus_tag="VFU90_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5478312.1"
                     /translation="LANKALPPTNGEPWIVSDAETVRWNDAGGAFVDVLEFERLAAAR
                     DTHSQAVDLYAGDLLENVYEDWVVAERERLRTLYLGVLDDLIGRYRAERNYADAIACA
                     KRVLANDAWREDTLRVLMALRYESGDTAGALAEYEKFAKRLRDELAVTPMPETVAVRQ
                     SILRQEAVPGAVSPSPSAPVAQRRGPGAILPFVGRKKELAALYAAWSRAARGCAGLVV
                     IAGEAGVGKTRLAAELARIVQSEGGRIFVGTTASPESMPYQPIVEALRSGLPLLLARN
                     MSPERRTVLAPLLPEVRDPDVPEVLFSETSPDRATARIYEALSFAVRTLASPRPLLLI
                     LEDLHWCGSATIEALGSILRDATRSPMLVVATCRQEETPVAHPLRALERSLRVNFNEE
                     ELELERLDEGDVSELVDRVEGLTGKSQDLARALYAQSEGNALFLDEAISAILENREPM
                     LGAPTMSSVIGRRIARLGRDGRTVVEIAAVAGSGCSVALIRDVSNLSSTAIARGLDEL
                     LDHRILREAGARAGYDYVFSHHLIADAVYAEIEPALRQQRHHRVAECLQAMFESNGSG
                     SAREVARHYESAGSQARAAEWYLRAAQGAAAVHAHGDAIELAGRSLQNATTDDLRRAA
                     FEVRETARSRHGDRQGQRDDIDALERLAKRDRRARFDVLLRRVFLARALGDSNDEYRY
                     IERLERLAAELGDSERAQALTQAATHLALCSRSAEAVEPARRALAIYEGCGDVKGQLD
                     CLCLLVEATANIGDLDAEKGYLNALRERAGSVSDRAAEARALGIAAIAALHRQAYRDC
                     FSLTERSLRIYVELNDREGEAAARGRLAVTAAWLRDYTFALREFEATLRMYESLGHKR
                     GLAVTHTNRTLLLMRLGFFSDALRSIERSNELFETVQEKRTVVVNRVNASFVNLQLGN
                     AAQAKALASSALASARELAYPVFEAAA"
BASE COUNT          470 a          948 c          936 g          471 t
ORIGIN      
        1 atttggcgaa caaggcgctg ccgccgacga acggggagcc gtggatcgtc agcgatgcgg
       61 aaacggtacg ctggaacgac gcgggcggcg cgttcgtcga cgttctcgag ttcgaacggc
      121 tcgccgccgc ccgcgatact cactcgcaag cggtcgatct ctacgccggc gatctgcttg
      181 agaacgtata cgaagattgg gtcgtcgccg agcgcgaacg gctgcgcacg ctctacctcg
      241 gcgtactcga cgatctgatc ggccgatacc gggcggaacg gaactacgcc gacgcgattg
      301 cttgcgcgaa acgcgtactc gccaacgacg cctggcgcga agacacgctt cgagtcttga
      361 tggcgctgcg ctacgaatcg ggcgacaccg ccggcgccct ggccgaatac gagaagtttg
      421 cgaaacgttt gcgggacgaa ctcgcggtta cgccgatgcc cgaaacggtc gccgtgcgcc
      481 aatcgatcct tcgtcaggaa gccgtgcccg gcgccgtaag cccttcaccg agcgcgccgg
      541 ttgcgcaacg ccgcggtccc ggcgcgattc tgccgttcgt cggacgtaag aaggagctcg
      601 cggcgctcta cgccgcttgg agtcgcgccg cccgcggctg cgccggtttg gtcgtcatcg
      661 caggtgaagc gggcgtcggc aagacgcggc tcgcggccga gctcgcgcgg atcgttcagt
      721 ccgagggtgg tagaattttc gtcggcacga ccgcctctcc ggaatcgatg ccgtatcagc
      781 cgatcgtcga agcgcttcgc tccggcctgc cgctgctgct tgcgcgcaac atgtcgcccg
      841 aacgccgcac cgtcttagcc ccgctcctcc cggaagttcg cgatcccgac gtccccgaag
      901 tgctcttttc ggaaacgtcg ccggatcgtg ctacggcgcg catctacgaa gcgctgtcgt
      961 ttgccgtccg aaccctggcc tcgccgcgcc cgctgctcct gatccttgaa gacctgcact
     1021 ggtgcggttc ggctacgatc gaggcgctgg ggagcattct ccgcgatgcg acgcgctcgc
     1081 cgatgttggt cgtcgcgacg tgccggcaag aagaaacgcc ggtcgcgcac ccgttacgcg
     1141 cgctcgagcg ttccttgcga gtcaatttta acgaagaaga gctcgagctc gagcgtctcg
     1201 acgaaggcga cgtgagcgag ctggtcgatc gcgtcgaagg tctgaccggg aagagccagg
     1261 acctcgcgcg cgcgctgtac gcgcaaagcg agggcaacgc gctctttctc gacgaggcga
     1321 ttagcgcgat tttggaaaac cgcgagccga tgctcggtgc gccgacgatg tcgagcgtta
     1381 tcggccgccg tatcgcgcgt ctcggccgcg acgggcgcac cgtggtggaa atcgcggcgg
     1441 tcgccggttc cggctgcagc gtcgccttga ttcgcgacgt ttcgaatctc tcgagcaccg
     1501 ccatcgcgcg cggcctcgac gaactgctcg atcaccgcat ccttcgcgag gctggggcgc
     1561 gagccggcta cgattacgtc ttcagccatc acctgatcgc cgacgcggtc tatgcggaaa
     1621 tcgaaccggc cctgcgacag cagcgccacc atcgcgtggc cgaatgcctg caggcgatgt
     1681 tcgagtcgaa cggctcgggt tcggcgcgcg aggtcgcgcg ccactacgaa tcggccgggt
     1741 cgcaggcgcg cgccgccgag tggtacctgc gcgcggcgca aggcgccgcc gccgtccacg
     1801 cccacggcga cgcaatcgaa ctggccggcc ggtcgctgca aaacgctacg acagacgatt
     1861 tacgccgggc ggccttcgaa gtgcgcgaga cggcgcgcag ccgccacggc gaccggcagg
     1921 gacaacgcga cgacatcgat gctctcgaac ggttggcgaa gcgcgaccgc cgcgcgcgct
     1981 tcgacgtgct gctgcggcgg gtgtttctcg cccgcgcttt aggcgactca aacgacgagt
     2041 accgctatat cgaacgcctc gagcggttgg ccgcggaact cggcgattcc gaacgggcgc
     2101 aagccctcac ccaagccgct acccacctcg ccctttgcag ccggtcggcg gaggcggtcg
     2161 agcccgcccg ccgggcgctg gcgatttacg aagggtgcgg cgacgtgaaa ggtcagctcg
     2221 actgtctgtg tctgctcgtc gaggcgacgg caaacatcgg cgatctggac gccgagaagg
     2281 gctacttgaa cgccctccgc gagcgggccg gcagcgttag cgatcgcgcc gccgaagccc
     2341 gagcgctcgg catcgcggcg atcgccgcgc tgcatcggca agcatatcgg gactgcttct
     2401 cgctgaccga acgttcgctc cggatttacg tcgagctcaa cgaccgcgaa ggcgaggccg
     2461 ccgcgcgcgg ccgcctggcg gtgacggcgg cgtggctgcg cgactatacg ttcgcgttgc
     2521 gagagttcga agcgacgctg cgaatgtacg agtcgctggg gcacaaacgc ggcttggccg
     2581 tcacccacac caatcgcact ttgctcctca tgcgcctggg ctttttctcg gacgccctgc
     2641 gttccatcga gcggtcgaac gagcttttcg aaacggtcca agagaagcgc accgtggtgg
     2701 tcaatcgcgt gaacgccagc ttcgtcaacc ttcaactcgg aaacgccgca caagccaagg
     2761 cgctggcgtc gtcggctttg gcgtcggcgc gcgaactggc ctatcccgtc tttgaagccg
     2821 ctgcg
//
LOCUS       DASUCJ010000063         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_566190, whole genome shotgun sequence.
ACCESSION   DASUCJ010000063 DASUCJ010000000
VERSION     DASUCJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566190"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFU90_00705"
     CDS             complement(<1..628)
                     /locus_tag="VFU90_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5478313.1"
                     /translation="MTIVERQDRFGVFGGRYVPETLVPALDQLAEEYDRAMADSGFRA
                     ELNSLLCTYVGRPSSLSDAPRFSELVGAPVYLKREDLNHTGAHKINNTVGQAMLARRM
                     GKRRIIAETGAGQHGVATATVCARFGLECVVYMGEEDMRRQALNVFRMKLMGAEVRPV
                     TSGTRTLKDATSEAIRDWVTNVTDSHYIIGSVVGPAPYPRIVRDLQSVI"
     gene            complement(625..>1061)
                     /locus_tag="VFU90_00710"
     CDS             complement(625..>1061)
                     /locus_tag="VFU90_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5478314.1"
                     /translation="LRLDVAQLHDAPSPDTVRALRQALDSPTAVWAVVRVSDAQAAAH
                     SIAELDGVADGILLDAAAAGSLGGSGVAFDWSAFPAHAQPRRSTFIVAGGLTPANVAG
                     AVRTFHPDVVDVSSGIERTRGVKDRELMSAFARAVLLESQRV"
BASE COUNT          174 a          362 c          346 g          179 t
ORIGIN      
        1 cgatcaccga ttgcaggtcg cgcacgatgc gcggataggg agccggcccg accaccgagc
       61 cgatgatgta atggctatca gtcacgttcg ttacccagtc gcgaatcgct tcgctcgtgg
      121 catccttcaa cgtcctcgtc ccggacgtca ccggacgcac ctccgcgccc atgagcttca
      181 tccggaagac attcagcgcc tgacgccgca tgtcctcctc gcccatgtac accacacact
      241 cgagaccgaa ccgcgcgcac accgttgccg ttgccacgcc gtgctgaccg gcgccggtct
      301 cggcgatgat ccgccgcttg cccatccgcc gggcgagcat cgcttggccg acggtgttgt
      361 tgatcttgtg ggcgcccgtg tggttgaggt cttcccgttt gaggtagacg ggtgctccaa
      421 ccagctccga gaaacgcggc gcatcgctca acgaggatgg acgaccgacg taggtgcaga
      481 gcaaactgtt cagctcggcc cgaaatccac tgtcggccat ggcgcgatcg tactcctccg
      541 cgagttggtc gagggccggg acgagggtct cgggcacata gcgcccgccg aagactccga
      601 agcgatcctg tcgttccacg atggtcatac gcgttgactc tcgagtagga cggcgcgcgc
      661 gaaggcgctc atcagttcgc ggtctttcac gccgcgcgtg cgctcgatgc cggacgacac
      721 gtcgacgaca tcgggatgga aggtgcgaac ggccccggcc acgttcgccg gcgttagtcc
      781 cccggcgaca atgaacgtgc tgcggcgtgg ttgcgcatgg gcgggaaacg cgctccagtc
      841 gaatgcgacg cccgatccgc cgaggcttcc cgccgctgcg gcatcgagca ggatgccgtc
      901 ggccacccca tcgagctcgg cgatgctgtg cgcggcggct tgggcgtcgg acacgcggac
      961 gaccgcccac acggctgtcg gcgagtcgag ggcctgtcgc agcgcgcgca cggtgtccgg
     1021 cgacggcgcg tcgtgcagct gcgctacgtc taaccggaga c
//
LOCUS       DASUCJ010000064         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1177591, whole genome shotgun sequence.
ACCESSION   DASUCJ010000064 DASUCJ010000000
VERSION     DASUCJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177591"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFU90_00715"
     CDS             <1..259
                     /locus_tag="VFU90_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU5478315.1"
                     /translation="PETINTVPPETLQLFEEHGVVRRTLSPRADEARDTMRQIADAGI
                     DFDDVTQTLEDQGIEKFEASYRKLLGVIAGKRQALGVASRA"
     gene            256..891
                     /locus_tag="VFU90_00720"
     CDS             256..891
                     /locus_tag="VFU90_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU5478316.1"
                     /translation="MTASGAMHDHGITHLFFDVGGVLGTSGWSTADRAEAAAHFCYDG
                     QEAERRHDGVVSALESGAMTLDEYLDHTVFFQPRPFTREAFTAFMRSRSVPNADTIAL
                     ARALAATGRYHMVTLNNESEALNVYRIACFGLTDIFDAFLSSCWLGVSKPARRAYRLA
                     LALTQAPPSRSVFIDDREENLEPARDAGLHTIRFTSAADLRSALRALGINF"
     gene            905..>1131
                     /locus_tag="VFU90_00725"
     CDS             905..>1131
                     /locus_tag="VFU90_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5478317.1"
                     /translation="MRLGMIGLGRMGGNMTRRLLRAKHDVVAFDRSADAVKELAGLGA
                     IAAASLADVCAKLSAPRIVWIMVPAGKPVDDT"
BASE COUNT          187 a          384 c          389 g          171 t
ORIGIN      
        1 accggagacg atcaacacgg tgccgcccga aacgctgcag ttgttcgagg agcacggggt
       61 ggtgcgccgc acgctgtcgc cgcgcgccga cgaagcgcgc gacacgatgc gccagatcgc
      121 cgacgcgggc atcgatttcg acgatgtcac gcagacgctg gaggaccaag ggatcgagaa
      181 gttcgaggcc tcgtatcgca aactgttggg cgtgatcgcg ggcaagcggc aagcgctcgg
      241 cgtcgcgtcg cgggcgtgac ggcatctggc gcgatgcacg accacggcat cacgcacctg
      301 ttcttcgacg tcggcggggt gttgggcacg tccggctgga gcaccgccga ccgcgcggag
      361 gcggccgcac acttctgcta cgacgggcaa gaggcggagc gccggcacga cggcgtcgtg
      421 agcgcgctgg aatccggcgc catgacgctc gacgaatatc tggaccacac ggtgttcttc
      481 cagccgcggc cgttcacccg cgaggcgttc accgcgttca tgcggagccg cagcgtgccc
      541 aacgcggaca cgatcgcgtt ggcgcgggcg ctcgcggcca cgggccggta ccacatggtc
      601 acgctgaaca acgagtcgga ggcgctcaac gtgtaccgca tcgcgtgctt cgggctcacc
      661 gacatcttcg acgcgttcct ctcgtcgtgc tggttaggcg tgtccaagcc cgcgcggcgc
      721 gcctatcggc tggcgctggc gctcacgcag gcaccgccgt cgcgcagcgt gttcatcgac
      781 gaccgtgaag aaaacctcga gccggcgcgc gacgccggcc tccacaccat tcgcttcacg
      841 tcggcggccg acctccggtc ggcgctccgc gcgttaggca tcaacttcta gcggacctgg
      901 aattatgcga ttggggatga tcggactcgg ccgcatgggc ggcaacatga cgcggcgcct
      961 cctccgggcc aaacacgacg tcgtcgcttt cgaccgcagc gcggacgcgg tgaaagaact
     1021 ggccgggtta ggcgcgatcg ccgccgcgtc gctcgccgac gtgtgcgcca agctgtcggc
     1081 gccgcgcatc gtatggatca tggtgcccgc cggcaaaccc gtcgacgata c
//
LOCUS       DASUCJ010000065         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1315028, whole genome shotgun sequence.
ACCESSION   DASUCJ010000065 DASUCJ010000000
VERSION     DASUCJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315028"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /gene="atpH"
                     /locus_tag="VFU90_00730"
     CDS             <1..429
                     /gene="atpH"
                     /locus_tag="VFU90_00730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU5478318.1"
                     /translation="HEDQTSKGFFLSPVIDRKEKEQVLSGVFSGRLHDIALHTLLLLV
                     RKRREALLGEIVRQYDALQMRARGEEPLSITSARELSAAELGSMVERLEKLYGKKFSV
                     TQTVQPNLIGGVRIMMGDRRIDGSVEGRLEELTRTLFAKT"
     gene            556..>1544
                     /gene="atpA"
                     /locus_tag="VFU90_00735"
     CDS             556..>1544
                     /gene="atpA"
                     /locus_tag="VFU90_00735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5478319.1"
                     /translation="MINADEIAGILKQQISNFKAGIQEDQVGTVIEVGANLARVYGLQ
                     GVRASELVEFPNGMQGVALNLEEDNVGVVIMGDDEEIKEGDKVRRTGRIASVPVGEAL
                     LGRVVDPLGNPVDGKGALETRKYRTIENVAPTVVARQSVKQPLPTGIRAIDALIPIGK
                     GQRELIIGDRSTGKTAIAIDTIINQKGRNVFCIYVAIGQKNSTIAALAQILEQKGAME
                     YTTIVSCGPADAAALRWIAPFAGAAMGEELMYSGKDVLIIYDDLTKHAQSYREMALLL
                     RRPPGREAYPGDVFFLHSRLLERAAKLSDELGGGSMTALPIIETQAGDFAAYIP"
BASE COUNT          331 a          475 c          484 g          254 t
ORIGIN      
        1 cacgaagacc agaccagcaa aggcttcttt ctctcgccgg taatcgaccg caaagagaaa
       61 gaacaggtgc tctccggcgt attctcgggc agactgcacg acatcgcgct gcacacgctg
      121 ctgctgctcg tgcgcaaacg gcgtgaggcg ctgctcggcg agatcgtccg gcaatacgac
      181 gcactgcaga tgcgggctcg cggcgaggag ccgctgagca tcacgtcggc gcgagaactc
      241 tccgccgccg agctgggttc catggtggag cgcctcgaga aactttacgg caagaaattc
      301 agcgtcacgc agaccgtgca gccgaacctg atcggcggcg tgcgcatcat gatgggcgac
      361 cgccgcatcg acggctcggt tgagggccgg ttggaagaac tgacgcgaac cctgttcgca
      421 aaaacctaat gacgaacgca aatttagggc gcccacgggg agcgctcaag acggtaacca
      481 caattaggcg ccctgcggaa atgggggccc cgtttacggg gggcgcgcag tctaggacgg
      541 agcgccttta aacatatgat taacgctgac gaaatcgctg gcatattaaa gcagcaaata
      601 tcgaacttca aggccgggat ccaagaggat caggtcggaa cggtcattga ggtcggtgcg
      661 aacctcgcgc gcgtgtacgg tttgcagggc gtgcgcgcgt cggaactcgt cgagttcccg
      721 aacggcatgc agggcgtcgc gctcaacctc gaagaggaca acgtcggcgt cgtcatcatg
      781 ggcgacgatg aagagatcaa ggaaggcgac aaagtccgcc gcaccggccg catcgcgtcg
      841 gttcccgtcg gtgaagcgct gctcgggcgc gtcgtggatc cgctcggcaa ccccgtcgac
      901 ggcaaaggcg ccctcgaaac gcgcaagtac cgcacgatcg aaaacgttgc gccgaccgtc
      961 gttgcgcgcc agtcggtcaa gcagccgctg ccgaccggca tccgcgcgat cgacgcgctg
     1021 atcccgatcg gtaaaggtca gcgcgaattg atcatcggcg accgttcgac gggtaagacc
     1081 gcgatcgcga tcgacacgat catcaatcaa aaaggccgca acgtcttctg catctacgtc
     1141 gctatcggtc agaagaactc gacgatcgcc gcactcgcgc aaatcctcga acagaagggc
     1201 gcgatggagt atacgaccat cgtatcgtgc ggaccggccg acgcggccgc gctgcgctgg
     1261 atcgcgccgt tcgccggcgc cgcgatgggc gaagagctca tgtactcggg caaagacgtc
     1321 ctcatcattt acgatgatct caccaagcac gcgcagtcgt atcgcgagat ggcgctgctg
     1381 ctgcgccgtc cgccgggtcg tgaagcctat ccgggcgacg tgttcttctt gcactcgcgc
     1441 ctgctcgaac gcgcggcgaa actctccgat gaactcggcg gcggctcgat gacggcgctg
     1501 ccgatcatcg aaacgcaagc cggtgatttc gcagcctaca ttcc
//
LOCUS       DASUCJ010000066         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_184031, whole genome shotgun sequence.
ACCESSION   DASUCJ010000066 DASUCJ010000000
VERSION     DASUCJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184031"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFU90_00740"
     CDS             <1..484
                     /locus_tag="VFU90_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478320.1"
                     /translation="ATPLVALSMRGTPAFAQRMHMGSDTSGHDSAFAARQARGKMVMG
                     VDQSTSTHHFVDLPNGGRITLVRDSDDAAGAARIQHHLREIAAAFSSGDFSSPMLVHM
                     KDVPGTRVMAEKKALIHYSTRALDRGGELTIQSADPDAVRAIHEFLAFQRTEHHVPPT
                     "
     gene            663..>1629
                     /locus_tag="VFU90_00745"
     CDS             663..>1629
                     /locus_tag="VFU90_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5478321.1"
                     /translation="MPNRYRAATCALLLLVLAAGSAARAQDSVAYRNATLPVDARVRD
                     LLGRMTLQEKFWQLFMIPGDLDDSTHDFSHGIFGLQVDAKHAALPPNPPPTASLEKTT
                     RSGAGSTRPADTPTADDVAAAAARTDAERIDAIQRYFVLHTRLGIPIIPFDEGVHGLV
                     RDGATDFPAAIALAAAFDTSLVRQVAGVIADEAASRGIRQLLSPVANVATDVRWGRVE
                     ETYGEDPFLAARMTGIYVHALEHAGVIATPKHFVANVGAGGRDSYPIDVGRRTLEETY
                     FPPFRAALSNGAQSIMTAYNSVGGVPATQNHRLLTDVLRRDWGFTG"
BASE COUNT          273 a          579 c          510 g          267 t
ORIGIN      
        1 ggcaacgccg ctcgtcgcgc tatccatgcg tggcaccccg gcgttcgcgc aacgcatgca
       61 catgggttcg gacacgtcgg gtcacgactc cgccttcgcc gccaggcagg cgcgcggcaa
      121 gatggtgatg ggcgtcgacc agtcgacctc gacccatcac ttcgtcgatc tgcccaacgg
      181 aggacgcatc acgctggttc gcgactccga cgatgcggcc ggggcggcgc gcatccagca
      241 ccacctgcgc gagatcgcgg ccgccttttc ctccggcgac ttctcatcgc cgatgctcgt
      301 gcacatgaag gacgttcccg gcacacgagt catggcagag aagaaagcgc tgattcacta
      361 ctctacgcgt gcgctcgatc gcggcggcga gctgaccatc cagtcggcgg atccggacgc
      421 cgtgcgcgct atacacgagt tcctcgcctt ccaacgcacc gagcatcacg ttccgcctac
      481 gtaaataaac cttcggagaa tctcgtgaag gcggggtgac atgccgcgtg cgtgggcgag
      541 tgaaccggcg cctcgacctg tcgggctcga ggcacctgat ccttacagga ctctcggccg
      601 cgctccctcg ccggcaccgg tgcgcgccgg tatgattgcg cgtccgatta ggccgcgccc
      661 acatgccgaa tcgatatcga gccgcaacct gtgcactgct cctgctcgta ctcgccgcgg
      721 gcagcgccgc gcgtgcgcag gactcggtcg cctaccgcaa cgccaccctc ccggtcgacg
      781 cccgcgttcg ggacctcttg ggccggatga cactccaaga aaagttttgg cagttgttca
      841 tgattccggg cgatctcgac gacagtacgc acgacttctc acatgggatc ttcggccttc
      901 aagtcgacgc gaagcacgcg gcgcttcctc ccaatccacc tcccacggcg tctctggaaa
      961 aaacgacgcg ttccggagcc ggcagcacgc gaccggccga cacgccgact gcggacgacg
     1021 tcgcggccgc cgccgcccgc accgacgccg agcgcatcga tgccattcag cgctacttcg
     1081 tgctgcacac gcgactcggc atcccgatca ttccattcga cgagggcgtg cacgggctgg
     1141 tgcgagatgg agccacggac tttcccgccg cgatcgccct cgcagctgcg ttcgatacgt
     1201 cgctcgttag gcaggtggcc ggcgtcatcg ccgacgaagc ggcgagccgc gggatccggc
     1261 agctgctgtc gcccgtcgcg aacgtcgcca ccgacgtgcg gtggggccgc gtcgaggaga
     1321 cgtacggcga agatccgttt ctcgcggcgc ggatgaccgg catctacgtc cacgcgctcg
     1381 agcacgccgg cgtgatcgcg acgccgaaac atttcgtcgc caacgtcggt gcgggcgggc
     1441 gcgacagcta tccgatcgac gttggccggc gcacgctcga ggaaacgtac tttccgccgt
     1501 ttcgggccgc gctgtcgaac ggcgcgcagt cgatcatgac ggcgtacaac tcggtgggcg
     1561 gcgtgccggc cacgcaaaac caccgcctgc tcaccgacgt gctccgccgc gactggggat
     1621 tcacaggct
//
LOCUS       DASUCJ010000067         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_550957, whole genome shotgun sequence.
ACCESSION   DASUCJ010000067 DASUCJ010000000
VERSION     DASUCJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550957"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFU90_00750"
     CDS             <1..338
                     /locus_tag="VFU90_00750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5478322.1"
                     /translation="AQVIVYIGAISVLILFAIMLTQTKDAPARLVFQTQAIPAAIASV
                     IIAVVVALAIAATDWHAATERIRVATDALSSVLFNQYVLPFEIVSVLLLAAVIGGVFL
                     AKREERTGL"
     gene            335..646
                     /gene="nuoK"
                     /locus_tag="VFU90_00755"
     CDS             335..646
                     /gene="nuoK"
                     /locus_tag="VFU90_00755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5478323.1"
                     /translation="MSNSLDAYLVLSGIIFAIGTFGFLARRNAISMLMSIELMVNAVN
                     LAIVAFGTFVQQLQGASSVIAIIVMAVAAAEATVGLAIVIAIYRNRRTPLVDEYDSLR
                     Q"
     gene            687..>1037
                     /locus_tag="VFU90_00760"
     CDS             687..>1037
                     /locus_tag="VFU90_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478324.1"
                     /translation="MDTLIPLIPALPIAGFDFTALFGRRLGRRASIVPIAAVVISWLV
                     AMTVVYAAFTNQAPFAEGKGVDVHLWNWIPAGNFHVDFGFYVDTLTACLLIVVTTVGM
                     LVHIYSVGYMGHDPG"
BASE COUNT          145 a          367 c          333 g          192 t
ORIGIN      
        1 ccgcgcaggt gatcgtgtac atcggcgcga tcagcgtcct gatcctcttc gcgatcatgc
       61 tgacccagac caaggacgcc ccggcccgcc tcgtcttcca gacccaggcg atcccggcgg
      121 cgatcgcctc ggtgatcatc gccgtcgtcg tcgcgctcgc gatcgccgcg acggactggc
      181 acgcggccac ggaacggatc cgcgtcgcga cggacgcgct ctcgtcggtg ctgttcaacc
      241 agtacgtgct gccgttcgag atcgtcagcg tgctgctgct cgcggcggtg atcggcggcg
      301 tgttcctggc caagcgcgag gagcggacgg gcctgtgagc aactcgctcg acgcctacct
      361 cgtcctctcc gggatcatct tcgcgatcgg cacgttcggg ttcctggccc gccggaacgc
      421 gatctcgatg ctcatgtcga tcgagctgat ggtgaacgcc gtcaacctcg cgatcgtcgc
      481 cttcggcacg ttcgtgcagc agctccaggg cgcgtcgtcg gtcatcgcga tcatcgtcat
      541 ggccgttgcg gccgcagagg ccaccgtggg gctcgcgatc gtcatcgcga tctaccgcaa
      601 ccggcggacg ccgctcgtcg acgaatacga ctccctgcgc cagtgacgga cgcccgcgcc
      661 gcgcactgcg gcggaacgaa ccactgatgg acacgctgat tccgctgatt ccggccctgc
      721 cgattgccgg cttcgacttc acggcgctgt tcggccgccg gctcggcagg cgtgcgtcga
      781 tcgtgccgat tgccgccgtc gtcatctcgt ggctcgtcgc gatgacggtg gtctacgcgg
      841 cgttcacgaa ccaggccccg ttcgccgagg gcaagggcgt cgacgtccac ctctggaact
      901 ggatcccggc cggcaacttc cacgtcgact tcgggttcta cgtcgacacc ctgacggcgt
      961 gcctgctgat cgtcgtcacg acggtcggca tgctcgtgca catctactcg gtcgggtaca
     1021 tgggccacga cccgggg
//
LOCUS       DASUCJ010000068         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_611953, whole genome shotgun sequence.
ACCESSION   DASUCJ010000068 DASUCJ010000000
VERSION     DASUCJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611953"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="cysC"
                     /locus_tag="VFU90_00765"
     CDS             <1..928
                     /gene="cysC"
                     /locus_tag="VFU90_00765"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU5478325.1"
                     /translation="PGLGHALQATVVWLGADPAMAGDAFRLRLGTRDVSVTLQRIDEV
                     IDPDTLVGKAGETVAKGDVAVVTLASRELIAADDELDDTSIGRFVLMHGTSVVAGGRV
                     RSIIGRPRSEGATDVIAQLSSVTSEERKTRNGHAGAVFWLTGLPSAGKSTVAMAVQRM
                     LFDRGRHVYVLDGDTLRTTLNVDLGFSEEDRSENVRRTAAVAGALADAGLVVICALIS
                     PFAADRERARAAYPGRFFEIYISCDLETAESRDVKGHYKRARLGEIPRFTGISSPYEP
                     PADPDLTLDTTKHSISESAGSLLEYIEDITRS"
     gene            complement(935..1939)
                     /gene="rfbB"
                     /locus_tag="VFU90_00770"
     CDS             complement(935..1939)
                     /gene="rfbB"
                     /locus_tag="VFU90_00770"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5478326.1"
                     /translation="MRWLVTGGLGFIGSNFIRMALRERPELRIVNLDAMTYAGNPANL
                     RDLESDPRYAFFKGSICDPESVRAAAGDGVDAIVNFAAETHVDRSILDPAQFLQTDIL
                     GPQVLLEAARERGVPRYVHVSTDEVYGDVAEGASVETDPLRPRSPYSASKAGGDLLVL
                     AYHATYGTPVSITRGSNTYGPYQYPEKLVPLFVTTLIDDGAVPLYGDGLQVRDWIHVD
                     DHARGILHVLDHGRPGEVYNLGGGNPRTNLEMTRLLVADCGRSMETNVRHVADRAGHD
                     RRYALDCAKLRALGWSPRVEFDEGIRETIAWYRANQDWWRPLKSGEFLEYYERQYVRR
                     "
     gene            2148..2936
                     /gene="rpsB"
                     /locus_tag="VFU90_00775"
     CDS             2148..2936
                     /gene="rpsB"
                     /locus_tag="VFU90_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5478327.1"
                     /translation="MSVVTMRELLEAGVHFGHQTRRWNPKMKPFIFQERNGIYIIDLA
                     LTVQKLRETIEVVKQLSREGRVILVVGTKEQAQDAVREEAERAGTFFVNQRWLGGTLT
                     NFATIQKRIARLRELENMKAQGDFDRLPKKEVAKLQDEMNKLERFLGGIKDMHRLPDA
                     IFIVDPKKERIAVLEARKLGIPIIAVIDTNCDPDEIDYPIPGNDDAIRAVKLMVGKIA
                     DAIVEGRAESESAYEEGVYAEPAAEGFADEMPVTLADAEAAAGV"
     gene            2952..3560
                     /gene="tsf"
                     /locus_tag="VFU90_00780"
     CDS             2952..3560
                     /gene="tsf"
                     /locus_tag="VFU90_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5478328.1"
                     /translation="MSTTTSYQPKADEIKALREETSAPMMDCRKALVDAAGDFQKAKG
                     LLLERGAAQAAKKADRTAKEGTVASYIHAGGKIGVLVEVNSETDFVARNPKFAELSRD
                     IAMHIAAMSPDYLDRESVPAEVVERARAEFRAGVPAGKPAEVAERIVEGKLNKWFEEH
                     CLLDQAFVKDDALSVGDLINGAIGALGEKISVRRFTKYALGE"
     gene            3566..>4318
                     /gene="pyrH"
                     /locus_tag="VFU90_00785"
     CDS             3566..>4318
                     /gene="pyrH"
                     /locus_tag="VFU90_00785"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5478329.1"
                     /translation="MPSARPRFSRVLLKISGEAFSGDESGVDVDTTRAMAEQIAEVSR
                     GGVSIAVVVGGGNIWRGKIHEEAGMDRASADYMGMLATVINALALQDALERMGVPSRV
                     QTAIAMHQIAEPYIRRRAIRHLEKGRVVIFAAGTGNPYFTTDTTAALRAVEVNAEAIL
                     KATKVDGIYSADPKKDPTATRFDRLEYMEVLQRGLDVMDSTAMALCMDNALPVIVFDM
                     AVPDNIRRVIWGEAIGTYVGRAQAAPAATPEAE"
BASE COUNT          809 a         1356 c         1462 g          691 t
ORIGIN      
        1 ccccggattg ggacacgcgc tgcaagccac ggtcgtgtgg ctcggtgcgg atcccgccat
       61 ggcgggcgac gccttccggc tccgcttggg aacgcgcgac gtctcggtga cgctgcagcg
      121 gatcgacgaa gttatcgatc ccgacacgct ggtcggcaag gcgggcgaaa cggttgccaa
      181 gggcgacgtc gcggtcgtaa cgctcgcctc gcgcgaactg atcgccgccg acgacgagct
      241 cgacgacacc agcatcggac gtttcgtgct gatgcacggt acgtcggtgg tcgcgggcgg
      301 gcgagtgcgc tcgatcatcg gccggccgcg atccgaaggt gcgaccgacg tcatcgcgca
      361 gctgtcgtcg gtcacttcag aagaacgaaa gacgcgcaac ggtcacgccg gcgcggtctt
      421 ctggctcacc ggtttgccga gcgcgggcaa gtcgaccgtg gcaatggcgg tgcagcgcat
      481 gctgttcgat cgcggacggc acgtctacgt gctcgacggc gacaccctgc gcacgaccct
      541 caacgtcgat ctcggttttt cggaagagga ccgcagcgaa aacgtccgcc gtaccgccgc
      601 cgtcgccgga gcgctcgccg atgccggtct ggtcgtcatc tgcgcgttga tctcgccgtt
      661 tgcggcggat cgcgaacgcg cgcgcgccgc atatcccggg cgattcttcg aaatttatat
      721 ttcgtgcgac ctggaaactg cggagtcgcg ggacgtcaaa gggcactaca agcgggctcg
      781 gctcggcgag atcccgcgct tcaccggcat ctcgtcgccg tacgagccgc cggcggatcc
      841 cgatttgacg ctcgatacga ccaagcactc gatttcggag tcggccggct ccctgctgga
      901 atacatcgaa gacatcaccc gttcgtaagc cgcctcaacg gcgaacgtac tgacgctcgt
      961 agtattcgag aaactcgccg gatttgagcg gccgccacca gtcttggttg gcgcggtacc
     1021 aggcgatcgt ttcgcggata ccttcgtcga actcgacgcg cggcgaccag ccgagcgcgc
     1081 gcagcttcgc gcagtcgagc gcgtaccgcc ggtcgtgacc ggcacggtcg gcgacgtggc
     1141 gcacgttggt ttccatcgaa cgaccgcagt cggcaaccag cagccgcgtc atctcgaggt
     1201 tggtgcgcgg attcccgccg ccgaggttgt agacttcgcc gggacggccg tgatcgagca
     1261 cgtgcaggat cccccgcgcg tggtcgtcga cgtggatcca gtcgcgcact tgcaggccgt
     1321 cgccgtagag cgggacggcg ccgtcgtcga tcagcgtcgt gacgaaaagc ggaacgagct
     1381 tctccggata ttgatacggg ccgtaggtat tgctcccgcg cgtgatcgag accggcgttc
     1441 cgtaggtcgc gtgataggcg agcaccagca gatcgccgcc cgccttgctg gcagaatagg
     1501 gactgcgcgg gcgtagcgga tcggtttcga ccgatgcgcc ctcggcgacg tcgccgtaga
     1561 cttcgtcggt cgacacgtgc acgtaacgcg ggacgccgcg ctctcgcgcg gcttccaaga
     1621 gcacctgcgg tccgaggata tcggtttgca gaaactgggc cgggtcgaga atcgagcggt
     1681 cgacgtgggt ctcggcggcg aagttcacga tcgcgtcgac gccgtcgccg gcggccgccc
     1741 gcacggactc gggatcgcag atgcttcctt tgaagaaggc gtagcgcgga tccgactcca
     1801 ggtcgcggag gtttgcgggg ttcccggcgt aggtcatcgc gtcgaggttg acgattcgca
     1861 gctcgggacg ctcgcgcagc gccatccgga tgaagttgga gccgataaag ccgaggccgc
     1921 ccgtcaccag ccaacgcatg gcttgcgcgt tcgtgaaggg agggtggcct gcctggaggc
     1981 cggcatagac cggggtaagg aagccgtgct ataatcgcgc ggctgccgcg gcgccggggg
     2041 tgccgcgctc gtacttcacc cggcatggag gctgggtgcg atggttccgc gacacgaccg
     2101 caatgcccgg atggcgcgcg gccgggaacc agaagggagt ctttgtcatg tccgtcgtta
     2161 cgatgcgcga gctgctggaa gcgggcgtcc atttcgggca ccagacgcgc cgctggaatc
     2221 cgaagatgaa accgttcatc tttcaggagc gcaacggcat ctacatcatc gacctggcgc
     2281 tgacggtcca aaagctgcgg gagacgatcg aagtcgtcaa gcagctctcg cgcgagggtc
     2341 gagtcattct ggtcgtcggc accaaggaac aagcgcagga cgccgtgcgc gaggaagccg
     2401 agcgggccgg aacgtttttc gtcaaccagc gctggctcgg cggtacgctg acgaacttcg
     2461 ccaccattca gaagcgaatc gcccgcttgc gcgagctgga aaacatgaag gctcagggcg
     2521 acttcgaccg tcttcccaag aaagaagtcg cgaagcttca ggacgagatg aacaagctcg
     2581 agcgttttct gggcggcatc aaggacatgc accgcttgcc cgacgcgatc ttcatcgtcg
     2641 atccgaagaa agagcgcatc gccgtgctgg aagctcgcaa actcggcatc ccgatcatcg
     2701 ccgtgatcga cacgaactgc gatcccgacg aaatcgacta tccgattccg ggcaacgacg
     2761 atgccatccg cgcggtgaaa ctgatggtcg gtaaaatagc cgacgcgatc gtcgaagggc
     2821 gcgccgaaag cgagagcgct tacgaagaag gcgtctatgc cgagccggca gcggaaggat
     2881 ttgccgacga gatgcccgtg acgctggccg acgccgaagc cgccgccggc gtttaacgag
     2941 agggagaaaa ggtgtccacc acaacgtcct atcaaccaaa ggccgacgaa atcaaagcgc
     3001 tgcgtgagga aacgagcgcg ccgatgatgg attgccgcaa agcgctggtc gacgcggcgg
     3061 gcgattttca gaaagccaag gggctgctcc tggagcgcgg tgccgcgcaa gccgcgaaga
     3121 aggccgaccg caccgcgaag gaaggcaccg tcgcgagcta catccacgcc ggcggcaaga
     3181 tcggggtgct cgtcgaggtc aacagcgaaa ccgacttcgt cgcgcgcaac ccgaagttcg
     3241 cggagctctc gcgcgacatc gccatgcaca tcgccgccat gtccccggat tacctcgacc
     3301 gcgagagcgt tccggccgag gtggtcgagc gagctcgcgc cgagtttcgc gccggggtcc
     3361 cggccgggaa gcccgccgaa gtcgccgagc ggatcgtgga aggcaagctc aacaagtggt
     3421 tcgaggagca ctgcctgctc gatcaggcgt tcgtgaaaga cgacgcgctc agcgtcgggg
     3481 acctgatcaa cggagcaatc ggcgcgctcg gcgaaaagat cagcgtgcga cgcttcacga
     3541 agtacgcgct cggggagtaa ccgcggtgcc ctccgctcgt ccgcggttct cgcgcgtcct
     3601 gctcaagatc tccggagaag cgttctccgg ggacgagtcc ggcgtcgacg tcgacacgac
     3661 gcgcgccatg gccgaacaga tcgccgaggt cagccgcggc ggcgtttcga tcgcggtggt
     3721 cgtgggcggc ggaaatatct ggcgcgggaa gatccacgag gaggccggca tggaccgcgc
     3781 gagcgccgac tacatgggca tgctcgcgac cgtcatcaac gcactcgcgc tgcaagacgc
     3841 gctcgagcgc atgggcgtcc cgtcgcgcgt acaaaccgcg attgcgatgc atcagatcgc
     3901 cgagccgtac attcgccgcc gcgcgatccg gcatttggag aagggccggg tcgtgatctt
     3961 tgcggccggc accggcaatc cgtacttcac caccgatacg accgccgcgc tgcgcgcggt
     4021 cgaagtgaac gccgaggcga ttctcaaggc gaccaaggtc gacggtattt attccgccga
     4081 tcccaagaaa gacccgaccg cgacgcgctt cgaccggctc gagtacatgg aagtgctgca
     4141 gcgtgggctg gacgtgatgg attccacggc gatggcgctg tgcatggaca acgcgctgcc
     4201 cgtcatcgtg ttcgacatgg cggtgcccga caatatccgg cgcgtcattt ggggcgaagc
     4261 gatcggaacc tacgtgggcc gcgcgcaggc cgcaccggcc gcgacgccgg aagccgag
//
LOCUS       DASUCJ010000069         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_214727, whole genome shotgun sequence.
ACCESSION   DASUCJ010000069 DASUCJ010000000
VERSION     DASUCJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214727"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFU90_00790"
     CDS             complement(<1..684)
                     /locus_tag="VFU90_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5478330.1"
                     /translation="MSGRLLIVSNRLPVTVSVSEHGGHPEVRASVGGLATGLRAPHEQ
                     SKGFWLGWPGQMSALDDAGRAEVQQQLEARRLVRVELTPEEVSVYYDRIANSVLWPVC
                     HDRLDQLPLRVEGWDVYESVNARFADAVAAVWQPGDRIWVHDYHLMRAPALIRERIPS
                     ARIGFFLHIPFPTPEIFFALARRRWLVSGMLGADLIGFHTRRHRGHFAAAVHRLLGTN
                     AHGNEFEHLG"
     gene            complement(681..>1186)
                     /locus_tag="VFU90_00795"
     CDS             complement(681..>1186)
                     /locus_tag="VFU90_00795"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU5478331.1"
                     /translation="VTGTVESIGLRSVRIRTAERSVVSMPNGKVADSRIETFAPRDRI
                     RFSCVLTLVYETTADQMRAVITGVEAMLKGSKHVAQDTITVFFLQLSPSSLDVQVAAQ
                     ILTTNGNEFNRIRQDLLLQIMDVVERAGTSFAFPTQTVQLESPPAAAATNGAAASNDA
                     AAPKPSS"
BASE COUNT          201 a          420 c          373 g          192 t
ORIGIN      
        1 gccgagatgc tcgaactcgt tgccgtgcgc gttggtgcct aacaatcggt gtacggccgc
       61 cgcgaagtgg ccgcgatgcc gccgcgtgtg aaagccgatc agatcggcgc ctaacatgcc
      121 gctcaccagc caccgccgtc gggcgagcgc gaagaaaatc tccggcgtcg gaaacgggat
      181 gtgcagaaag aacccgatgc gcgccgaggg aatccgctcg cgaatcagcg ccggggcccg
      241 catcaggtgg taatcgtgca cccagatgcg atcgcccggc tgccataccg ccgccacggc
      301 atcggcgaag cgcgcgttca cgctctcgta cacatcccaa ccttcgacgc gcagcggcag
      361 ctggtccagc cggtcgtgac acaccggcca cagcacgctg ttcgcgatcc gatcgtaata
      421 gacggacact tcctcgggcg tgagctccac gcgcaccagg cggcgtgcct cgagctgctg
      481 ctgcacctcc gcgcggccgg catcgtcgag cgcgctcatc tgcccgggcc agcctaacca
      541 gaatcccttc gactgctcgt gcggtgcgcg gagtccggtc gccaatccgc cgacgctggc
      601 ccggacctcg ggatgccccc cgtgttccga cacgctcacc gtcaccggta aacggttcga
      661 cacgatgagc aggcgtccgc tcacgaggag ggctttggcg ccgccgcgtc gttggacgcg
      721 gccgcaccgt tggttgccgc cgcggccggc ggcgattcga gttgcaccgt ctgcgtcgga
      781 aacgcgaagg acgtgcccgc gcgctcgacg acgtccatga tctgcaggag caaatcttgc
      841 cggatgcgat tgaactcgtt gccgttcgtc gtcagaatct gcgccgcgac ctgcacgtcc
      901 agtgacgacg ggctgagttg caggaagaag accgtgatcg tgtcctgcgc cacgtgcttc
      961 gagcccttga gcatcgcttc gacgcccgtg atcaccgccc gcatctggtc ggccgtcgtc
     1021 tcgtacacca gcgtgagcac gcaggaaaat cggatccgat cccgcggcgc gaatgtctcg
     1081 atccgcgaat cggccacctt tccgttaggc atcgacacca cgctgcgctc ggccgtccgg
     1141 atgcggaccg accgcagccc gatgctctcc accgtgccgg tcacgg
//
LOCUS       DASUCJ010000070         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1299872, whole genome shotgun sequence.
ACCESSION   DASUCJ010000070 DASUCJ010000000
VERSION     DASUCJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299872"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFU90_00800"
     CDS             <1..382
                     /locus_tag="VFU90_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5478332.1"
                     /translation="GTEPWGILGKAHPHPRAYGTFPQIIQKYVEQQHALTLPDAIRKF
                     TSLAAQRMGLTQRGVIKAGMYADIVVFDPAKVVATATFEQPNQLAKGMDYVIVNGVPV
                     IADGKMTHALPGKVLRGPGYVPGK"
     gene            445..>1200
                     /gene="dapF"
                     /locus_tag="VFU90_00805"
     CDS             445..>1200
                     /gene="dapF"
                     /locus_tag="VFU90_00805"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5478333.1"
                     /translation="MGLRFTKMHGLGNDFVVLDARTTQVVLDVARIRAIADRHTGVGF
                     DQMMILEPARDLSCIAAYTIRNADGTVAPQCGNGARCLGAWLHRAGVLPIGVEVMLQS
                     PVGIVRMRLADATTVSVEMGEPEFEPARIPFTAPVAADTYPLDVDGERVEISAASIGS
                     KHAVIEVDDIASARVDRLGPRITVHPRFPQGANACFVRVVDEHSVKLRVHERGAGWTL
                     ACGSGACATVAALHRRGRVGDDVRVEMPGGALAI"
BASE COUNT          215 a          421 c          389 g          175 t
ORIGIN      
        1 aggtaccgaa ccgtggggaa tcctcggcaa ggcgcacccc cacccccgcg catacggcac
       61 gtttccgcag atcatccaga agtacgtcga acagcagcac gcgctgaccc tgcccgatgc
      121 gatccgcaag ttcacatcgc tggccgcgca acggatggga ctgacccaac gcggcgtgat
      181 caaggccggg atgtacgcgg acatcgtcgt gttcgatccc gccaaggtcg tcgccaccgc
      241 gaccttcgaa caacccaacc agctcgccaa gggcatggac tacgtcatcg tgaacggggt
      301 tccggtgatc gccgatggca agatgaccca cgcgcttccg ggaaaagtgt tgcgcgggcc
      361 gggatacgtg cccggcaagt gagcgccgta ccatccgatc gggcacacga tccgccggat
      421 cgccaacaac gtcgagaacg acacatgggc cttcgtttca ccaagatgca cggcctcggc
      481 aacgacttcg tcgtgctcga cgcgcgcacg acgcaggtgg tgctcgacgt cgcgcgcatc
      541 cgtgcgatcg cggaccgtca caccggcgtc ggcttcgacc agatgatgat cctggagccc
      601 gcgcgggacc tctcctgcat cgcggcgtac acgatccgca atgccgacgg caccgtcgca
      661 ccgcaatgcg ggaacggagc gcgctgcctc ggcgcgtggt tgcaccgcgc cggcgtgctg
      721 ccgatcggcg tcgaagtcat gctgcaaagc cccgtcggca tcgtcaggat gcgcctcgcc
      781 gacgccacga ccgtcagcgt ggaaatgggc gagccggagt tcgaacccgc gcgcataccg
      841 ttcaccgcgc cggtggctgc ggacacctat ccactcgacg tcgacggaga acgcgtcgaa
      901 atctcggcag cctcgatcgg aagcaagcat gcggtgatcg aggtcgacga cattgcatcg
      961 gcccgcgtcg atcgcctcgg ccctcgcatc acggtgcacc cgcgcttccc gcaaggcgcc
     1021 aacgcgtgtt tcgtgcgcgt cgtggacgaa cattccgtga agctgcgcgt gcacgagcgc
     1081 ggcgccgggt ggacgctcgc ctgcggcagc ggggcgtgcg cgacggttgc cgccctgcac
     1141 cgacgcggcc gcgtcggcga cgacgtgcgc gtggaaatgc ccggcggcgc gctcgccatc
//
LOCUS       DASUCJ010000071         1445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_367585, whole genome shotgun sequence.
ACCESSION   DASUCJ010000071 DASUCJ010000000
VERSION     DASUCJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367585"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VFU90_00810"
     CDS             <1..1246
                     /locus_tag="VFU90_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478334.1"
                     /translation="FYNGGPGSSTIWLRMGSFGPMRVLVGDGSATANAPFHLVPNEYT
                     LLDRSDLVFVDAPGTGFSRIVGAGKPDEFYGVDQDMRAFTQFVSRYVSTYGRWNSPKF
                     LFGESYGTPRTAVLVDNLQKQGIGINGIVLLSSVLDFNLDWGINFTPVSIGGGDWAFP
                     LYLPTEAASSWYHNALPGPQTTLQNLLPQVETFAMGEYLNALAAGDRLAPSTYDDVVA
                     KLHQYTGLSPQYIRSSNLRIPYWRYLTELQRNSGQTLGRYDARFTSYTLDRLADRPQF
                     DATDSAIDAAFVGAGNYYVRQLLRYNPPTDYLPLVFAVNRQWDWKHNGNLPTNTAQDL
                     AEAMTFNPSLRVFSANGYYDMATPFFATVYTLNHLSLAPQLQGNITYGFYESGHMVYL
                     HPAALAAFHADLERWYAQVLGH"
     gene            complement(1254..>1445)
                     /locus_tag="VFU90_00815"
     CDS             complement(1254..>1445)
                     /locus_tag="VFU90_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478335.1"
                     /translation="PENPDDSVGAYSHDFTSFLFLENALDYLESAVESGDAAPTPDMR
                     AAYAKLEAIYRKTLARLGQ"
BASE COUNT          258 a          467 c          444 g          276 t
ORIGIN      
        1 tttctataac ggcggcccgg gcagctcgac catttggctg cgcatgggtt cgttcggccc
       61 gatgcgcgtg ctggtcggag acggcagcgc gaccgccaac gcgccgttcc acctcgtccc
      121 gaatgaatac acgctgctgg accgcagcga tctggtcttc gtcgacgctc ccgggaccgg
      181 cttcagccgc atcgtcggcg ccggcaagcc ggacgaattt tacggcgtgg accaagacat
      241 gcgcgccttt acccagttcg tttcgcgcta cgtcagcacg tacggccgct ggaattcgcc
      301 gaagttcttg ttcggcgaat cgtacggaac gccgcggacc gcggtcctgg tcgacaacct
      361 gcaaaaacag ggcatcggaa tcaacggcat agtcctgtta tcgtcggttc tcgacttcaa
      421 cctcgattgg ggcatcaact ttacgccggt gtcgatcggc ggcggcgact gggcattccc
      481 gctctacttg ccgacggagg cggcctcgtc gtggtatcac aacgcactgc cgggtccgca
      541 gaccaccctg cagaacctcc tgccgcaagt cgagacgttc gcgatgggag aatacctcaa
      601 cgcgctggcc gcgggcgatc ggctcgcacc ctcgacgtac gacgacgtcg tagcgaagct
      661 gcatcagtat accgggctgt cgccgcagta cattcgcagc tcgaatctgc ggattccgta
      721 ctggcgctac ctgaccgaac tgcagcgtaa ttccgggcaa acgctcgggc gctacgacgc
      781 gcgctttacg tcctacacgc tcgaccggct tgcggatcgc ccgcagttcg acgcgaccga
      841 ctcggccatc gacgcggcgt tcgtaggcgc gggcaactac tacgttcgcc agctgctgcg
      901 ctacaatccg ccgacggact atctccctct ggtgtttgcg gtgaaccgtc agtgggattg
      961 gaagcacaac gggaatctcc cgaccaatac ggcgcaggat cttgccgaag cgatgacgtt
     1021 caatcccagc ctgcgggtgt tctcggccaa cggctactac gatatggcga cgccgttctt
     1081 tgcgacggtg tatacgctga accacctaag cctggcgccg cagctccagg gcaacatcac
     1141 gtacggattc tacgaatccg ggcacatggt ctatctccac ccggcggcgt tggcggcgtt
     1201 ccacgcggac ctggagcgct ggtacgccca ggtgctgggg cattgatcga gagctactgc
     1261 ccgagccgag ccagcgtctt acggtagatc gcctcgagct tagcgtacgc ggcgcgcatg
     1321 tccggcgtgg gcgcggcgtc gccgctttcg acggcgctct cgagatagtc gagagcgttt
     1381 tccaggaaga gaaagctcgt gaagtcgtgc gagtacgcgc cgaccgagtc gtccggattc
     1441 tcggg
//
LOCUS       DASUCJ010000072         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_352284, whole genome shotgun sequence.
ACCESSION   DASUCJ010000072 DASUCJ010000000
VERSION     DASUCJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352284"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..302)
                     /locus_tag="VFU90_00820"
     tRNA            complement(228..302)
                     /locus_tag="VFU90_00820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Thr,seq:ggt)
     gene            836..2002
                     /locus_tag="VFU90_00825"
     CDS             836..2002
                     /locus_tag="VFU90_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478336.1"
                     /translation="MKVDAKSALRFVFAMGVVNLFADMTYEGGRGVVGAFMGHLGASG
                     AIVGAVAGGGELAGYAIRSVSGAIADRTGRYWIDAWAGYLINMLCVPALALAGSWPAA
                     AGLVVGERVGRGIRRPVIAAVLSEAGRQIGGGHAFGLNEALDQIGATTGPLIVAYAVA
                     RGGFTAGFGVLIVPAMLTLACLSIATAFGKNLVPRDDGPLKPGIKDAPAFRRYAIGGA
                     LVAAGYVDFALIAFRFQRDGVAGTPAISIWFAVAMAVAAISAPLLGRLFDRRGTAVIG
                     VALAVTAAASPLAFLTTGAAAKLGVALWGVGTAVQDALLLALVATVIARGRKATAFGL
                     YDLIFGAAWFAGSAICGVLLDRSVVALVIFSAALQLLAIPFFVIVPAKPTPANA"
BASE COUNT          312 a          748 c          688 g          418 t
ORIGIN      
        1 cggtttccaa gtcggctcgg atcccggctc gccggttacc gatcgctata ccggttcgtt
       61 tccgttcgac gcgacgattc acaccgtcac cgtcgacgtc agcggcgacc tcatcaagga
      121 cgacaaagcc gagatggcgc ggctgctggc gcgtcagtaa cccggcgact caggctcgct
      181 ggaaagcaaa agcagctccc cggtatcggg gagctgcttg cgtcttctgg agccgttggc
      241 ggggattgaa cccgcggtct cgtctttacc aaagacgtgc tttaccacta agctacaacg
      301 gcacgccgag tccggctctc cttgtacttc gcaccccttc ccctccccta cctcagccca
      361 gtcgtcctcc cggaccagat aagcggaagc ccatcgcacc cggctgtact gcttgttctt
      421 cgtcggcaaa agccgcaacg tacccgtcgt gttcacgtca tcaatcggca ccaagaacac
      481 cgcctcgatt tctcggcaat acactgcgat gtattcgacc tctccggcat atgttctgat
      541 cgatgggcca tttcgatggg agtgcgagct ccacgggctg aatagcaccg ccccgtttcg
      601 gagccgcccg gtcttcacct gaacgcgcga taagatgccg tccttatcga cgatcaagtc
      661 gtagcgatgg ctgtcgccga acggcacggc gacgatgtag ccggcgcgga cgagcgcgcg
      721 cgccgcttcc atctccgacc ggtcgccgat gcttttggtg tcgcgcttct tcgatcgctc
      781 catgcgagcg atcgtaactt gctaagatgc catacgtgtg gcatcttttg tacagatgaa
      841 agtcgacgcg aagagcgccc tgcgtttcgt cttcgcgatg ggcgtcgtca acttgtttgc
      901 cgacatgacc tacgaaggcg ggcgcggcgt cgttggcgcg ttcatgggcc acctcggtgc
      961 gagcggcgcg atcgtcggcg cggtcgcggg cggcggcgaa ctcgccggct acgcgattcg
     1021 ctcggtctcc ggcgcgattg cggatcgcac cgggcgctac tggatcgacg cctgggccgg
     1081 ctatctcatc aacatgctgt gcgtgccggc gctcgcgctg gccggcagct ggcccgccgc
     1141 cgccggcctc gttgtcggcg aacgcgtcgg gcgcggcatc cgtcgtcccg tgattgccgc
     1201 cgtcctctcc gaagcggggc gtcaaatcgg cggcggccac gcattcggtt taaacgaagc
     1261 gctcgatcaa atcggcgcga ccaccggtcc gctgatcgtc gcctacgcgg tcgcgcgcgg
     1321 cggcttcacc gccggcttcg gcgtcctgat cgtgcccgcg atgttgacgc tcgcgtgtct
     1381 ttcgatcgca accgcgttcg gcaagaacct cgttccgcgc gacgacggtc cgctcaaacc
     1441 cgggataaaa gacgcaccgg cgttccgccg gtatgcgatc ggcggcgcgc tggtcgccgc
     1501 cggctacgtc gacttcgcgt tgatcgcctt tcgcttccaa cgcgacggcg tcgccggtac
     1561 gccggcgatc tcgatttggt ttgcggttgc gatggcggtc gccgcgattt ccgcgccgct
     1621 gctcgggcgg ctgttcgacc ggcgcggaac ggcggtgatc ggcgtcgcgc tcgcggtcac
     1681 ggccgcggcc tcaccgctgg ccttcctcac taccggcgcc gccgccaaac tcggcgtcgc
     1741 gctctggggc gtcggcaccg ccgtgcaaga cgcgctgctg ctcgcactgg tcgccaccgt
     1801 catcgcgcgc ggccgcaaag cgaccgcgtt cggcctctac gatctcatct tcggcgcggc
     1861 gtggtttgcc ggcagcgcga tttgcggcgt gctgctggat cgctcggtcg tcgcactggt
     1921 aatcttctcg gccgcgctgc agctgctcgc gattccgttt ttcgtgatcg tccccgcgaa
     1981 accaacgccc gcaaacgcct agcgcgcgtt acgtcggctt cggcgacggc gacggcgacg
     2041 cggccggtac tgccgccggc gaagatggcg acgcgacgga cgttgccgac ggcggcgttg
     2101 ccgacggcac catcgtcacc gccggagcgg ccgtcggcgg cgcggttgga cccggtagcg
     2161 acgctt
//
LOCUS       DASUCJ010000073         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_596817, whole genome shotgun sequence.
ACCESSION   DASUCJ010000073 DASUCJ010000000
VERSION     DASUCJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596817"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1172
                     /locus_tag="VFU90_00830"
     CDS             <1..>1172
                     /locus_tag="VFU90_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478337.1"
                     /translation="PATIYNSAARGQVDSEAAGLALFAIYCLLRSDDGAAERPSGWVV
                     AAWLAIAYSLLIKPQAAVVLPLMAAFVFVDPARRRARLIASGIGIGAAILLALVLVEP
                     FHPSNPVEAFSWLFGRYSYGSNVYPYNSVNAFNLWAIRGSFWQPDTGPISAFGTFSLG
                     PQYVWGIILVAAAVVLVLWRYVQERTPRALLEGCAVATIAFFVLATRMHERYLFDGLL
                     FTIVCVPFARRYLWGAVALSVVLFANLQYSLQYLYAMVNHVPGVNAQNLWGAGTTAFA
                     LVSVGTFFWLGYQFLGGETAPAPAAAGPEEARTPAVAAPAGLKTRNWFDPAEGLAAMR
                     WPLDYAVAAGFGAVNFVLSFVGYWWPPDKVFDEIYFARAGEEYLQNLRIYENTHPPL"
BASE COUNT          155 a          367 c          415 g          235 t
ORIGIN      
        1 ccggcgacga tttacaactc ggcggcgagg gggcaagtcg attcggaggc ggccggcttg
       61 gcgctgttcg cgatttactg tttgctgcgc tccgacgacg gcgcggccga acggcccagc
      121 ggttgggtcg ttgcggcgtg gctcgcgatt gcgtactcgc tgctgatcaa accgcaggcg
      181 gcggtcgtgc tcccgctgat ggcggcgttc gtgttcgtcg atccggcacg ccggcgcgcg
      241 cggctgatcg cctcggggat cggcatcggc gccgcgatcc tgctggcgct ggttctcgtc
      301 gagccgttcc atccgagcaa cccggtcgaa gcatttagct ggctgttcgg gcgctacagt
      361 tacggctcga acgtctatcc gtacaacagc gtaaacgcgt tcaacctgtg ggcgatccgc
      421 ggctcgttct ggcaacccga caccggcccg atcagcgcgt tcggtacgtt ctcgctcggg
      481 ccgcagtacg tctgggggat cattctggtc gccgcggcgg tggtgctggt gctatggcgg
      541 tacgtgcagg aacgcacgcc gcgcgcgctg ctggaagggt gcgcggtcgc gacgatcgcc
      601 tttttcgtgc tggcgacgcg gatgcacgaa cgctacctgt tcgacgggtt gctgttcacg
      661 atcgtctgcg tgccgttcgc gcggcgctac ctgtggggcg cggtggcgct gtcggtggtg
      721 ctgttcgcca acctgcagta ctcgctgcag tatttatacg cgatggtgaa tcacgtgccg
      781 ggcgtgaacg cgcagaactt gtggggcgcg gggacgaccg cgttcgcact cgtctcggtc
      841 ggcacgttct tctggctcgg ctatcaattt ttgggcggcg agaccgcccc ggcgccggcg
      901 gccgccggcc cggaggaggc gcgtacaccc gccgttgcgg cgccggcggg tctaaaaacg
      961 cgcaactggt tcgatcccgc cgaggggctg gcggcgatgc gctggccgct cgattacgcc
     1021 gtcgccgcgg gattcggcgc cgttaacttc gtgctgtcgt tcgtggggta ttggtggccg
     1081 cccgacaagg tcttcgacga gatctacttc gcgcgcgccg gcgaagagta tctgcagaac
     1141 ctgcggatct acgaaaacac ccatccgccg ct
//
LOCUS       DASUCJ010000074         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1452654, whole genome shotgun sequence.
ACCESSION   DASUCJ010000074 DASUCJ010000000
VERSION     DASUCJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452654"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..>1172
                     /locus_tag="VFU90_00835"
     CDS             162..>1172
                     /locus_tag="VFU90_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5478338.1"
                     /translation="MIGEDQVMPGPYGPRRVTYADYTASGRAVSFIEDFIRDEVLPRY
                     ANTHTESSGTGRQTTRLREDARAIIHETVAGTDDDVVIFAGSGATGAIAKLIGILGLR
                     IPSTLEDTHHLSAHIPRDQRPIVFIGPFEHHSNELPWRESIAHVVTIPQDADGHIDQA
                     VLERELIRHEHRPLLIGSFSAASNVTGILSDDAAIAELLHRHGALSFWDFAAAAPYVD
                     LRMRALGGRPESYKDAMFLSPHKFVGGPATPGVLVVRRDLLRNRVPDVPGGGTVEYVS
                     SAEHVYLSDPVAREEGGTPAIVGAIRAGLAFGLKAAVGVELIRANEERLLRRAIAAWK
                     DEP"
BASE COUNT          190 a          417 c          374 g          191 t
ORIGIN      
        1 gccgctgctt cagaatgcgg atgagtcgcc gaaacagaaa tcccatgtcg cacccttttt
       61 tcgtccggcc gattcttact gcgcagcgta atacgatccg agatatgcgg gaggctgccg
      121 acggcgcggg ggcgtcgttg ctggcgcgta tccgcgagtc gttgatcggc gaagaccagg
      181 tgatgcccgg cccgtacggc ccgcggcggg tcacctacgc cgactacacg gcgtccggcc
      241 gggcggtctc gttcatcgag gatttcatcc gcgacgaggt cttgccgcgc tacgcgaaca
      301 cccacaccga gtcgagcggc accggccggc agaccacccg gctgcgcgaa gacgcgcgcg
      361 cgatcatcca cgagacggtc gccggcaccg acgacgacgt cgtcattttc gccggctccg
      421 gggcgaccgg cgcgatcgcc aagctgatcg gcatcctcgg cctccggatc ccgtcgacgc
      481 tcgaggacac tcatcacctc tccgcgcaca tcccgcgcga ccagcgcccg atcgtgttca
      541 tcggaccgtt cgagcaccac tcgaacgagc tgccgtggcg cgagtcgatc gcgcacgtcg
      601 tgacgatccc gcaggacgcc gacggccaca tcgaccaagc ggtgctcgaa cgcgaactaa
      661 tccgccacga acaccggccg ttgctcatcg gttcgttctc cgccgccagc aacgtcaccg
      721 gcattctctc ggacgacgcc gctatcgcgg agttgctaca tcgtcacggc gccctctcgt
      781 tctgggattt cgccgccgcc gcgccctacg ttgaccttcg gatgcgcgct ctcggcggtc
      841 gcccggaatc ttacaaagac gcgatgttct tgagcccgca caagttcgtc ggcggtcccg
      901 ctacgccggg cgtgctcgtc gtccgaagag acttgctgcg caaccgggtg cccgacgtgc
      961 ccggcggcgg caccgtcgag tacgtcagtt cagcagagca cgtctacctg tccgacccgg
     1021 tcgcccgcga agaaggcggc accccggcca tcgtcggcgc gatccgagcc ggcttggcgt
     1081 tcggcctcaa ggcggcggtt ggcgttgagc tgatccgcgc gaacgaggag cgactgctgc
     1141 ggcgggcgat cgcggcgtgg aaagacgagc cg
//
LOCUS       DASUCJ010000075         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1437435, whole genome shotgun sequence.
ACCESSION   DASUCJ010000075 DASUCJ010000000
VERSION     DASUCJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437435"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFU90_00840"
     CDS             complement(<1..1094)
                     /locus_tag="VFU90_00840"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU5478339.1"
                     /translation="MLAAGLKPRREYWYRFIDDHGNASRVGRTLTAPDEHDTKPLNFA
                     FASCQNVQQGSCNAYRRMIWDDERSALDDRLSFVLHLGDFVYEIVWYPEDRPQGMYDR
                     RLRDIVRYPEGEKHADFHVPTTVHGYRALYRAYLADPDLQDARARWPFICMWDNHEFS
                     WKGWQSQENFGHGVVPAQTRKVAAAEAWWDYQPARVVKSGPGGMNTFTAPVVRDAPLG
                     RFDPHGLGEDPANLAAIGALTLYRTFKLGAHVELVLTDNRSYRSEPLTDRPEWAPFRQ
                     HAVPFFESEDALTVLDAGREAHGGTPPAAIEVGGTSLPNPRRASPPGTVLGMRQRAWF
                     LEHLRVSQAPWKIWGNSIGSLDWRTDLQHLP"
BASE COUNT          187 a          377 c          369 g          174 t
ORIGIN      
        1 ggcaggtgct gcagatcggt gcgccagtcg agcgagccga tcgaattgcc ccaaatcttc
       61 cagggcgctt gcgatacgcg cagatgctcg aggaaccacg cgcgttgccg catgccgagg
      121 acggtgccgg gcggcgaggc gcgtcgcggg ttcggcagag aggtcccgcc gacctcgatc
      181 gccgccggcg gcgtgccccc gtgcgcctcg cggccggcgt cgagcaccgt caacgcgtcc
      241 tcggactcga agaacggaac ggcgtgctgg cggaacggcg cccactcggg ccggtcggtg
      301 agcggctccg aacggtacga ccggttgtcc gtcagcacga gctcgacgtg cgcgcccaac
      361 ttgaacgtcc ggtacaacgt gagggcgccg atcgccgcga ggttcgccgg gtcctcgccc
      421 aaaccgtgcg ggtcgaaccg gcctaacgga gcgtcgcgca ccaccggcgc cgtgaacgtg
      481 ttcatgccgc ccgggccgga cttcaccacg cgcgcgggct ggtagtccca ccacgcctcg
      541 gcggcggcca ccttccgcgt ctgcgcgggc acgacgccat ggccgaaatt ctcctggctc
      601 tgccatccct tccacgaaaa ctcgtggttg tcccacatgc agatgaacgg ccaccgtgca
      661 cgcgcatcct gcagatcggg atcggcgaga tatgcgcgat acaacgcacg atatccatgc
      721 acagtcgtcg gcacgtgaaa gtcggcgtgc ttctcgcctt caggatagcg cacgatgtcg
      781 cggaggcgac ggtcgtacat gccctgcgga cgatcctcgg gataccaaac gatctcgtag
      841 acgaagtcgc ccaggtgcag cacgaaactc aagcgatcgt cgagagcgct gcgttcgtcg
      901 tcccagatca tgcgccggta cgcgttgcac gatccctgtt gcacgttctg gcacgacgcg
      961 aacgcgaagt tgagcggctt cgtgtcgtgc tcgtcgggcg ccgtgagcgt tcgacccaca
     1021 cgactcgcgt tgccgtgatc gtcgatgaac cggtaccagt actcgcggcg gggcttgagg
     1081 ccggcggcca gcacgcggca cgtccag
//
LOCUS       DASUCJ010000076         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_933105, whole genome shotgun sequence.
ACCESSION   DASUCJ010000076 DASUCJ010000000
VERSION     DASUCJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933105"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFU90_00845"
     CDS             <1..845
                     /locus_tag="VFU90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478340.1"
                     /translation="DGIVSLAGLVLSCTGIAQQFCQEGSDEYFLLNFGHGPAAGLMRC
                     YEPPAVLGSVALASAAIVAIAAYRREWFDAATAASARRLSQTVAAVLFAAFCGAIVAN
                     AISAARSAGAAPGFRAMERSVSTFAVDPIRRGGATGCALTFAANDVVVYADDPFAARF
                     ATASLGYTLFTPEAVRLRGRTIALQSLKSAFVNSDVRTIGGRSVRVTREFDLTRELRP
                     FRYVEQMFEVPCNLIADNPLLIYRFDFAAAQAAAARTPLLQRLNVFLQGNPAVRVDTG
                     RYHA"
     gene            838..>1042
                     /locus_tag="VFU90_00850"
     CDS             838..>1042
                     /locus_tag="VFU90_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5478341.1"
                     /translation="MPDALPRPAGVAVVPCFNEGSNPIGISRALLEIPDLTVAFVDDA
                     SDAPSREVLDSLAGRDARVRVVRN"
BASE COUNT          140 a          330 c          369 g          203 t
ORIGIN      
        1 gcgacggcat cgtaagcttg gccggcttgg tactgtcgtg tacggggatc gcgcagcagt
       61 tttgccagga aggcagcgac gaatacttct tgctgaattt cgggcacggc ccggcggccg
      121 gattgatgcg gtgttacgag ccgcccgcgg tattgggaag cgtcgcgctc gcgagcgcgg
      181 cgatcgtcgc gattgccgcg taccggcggg aatggttcga cgccgcgacc gccgcgagcg
      241 cgcggcgctt gagtcaaacg gtggccgccg tcctgttcgc ggcgttttgc ggtgcgatcg
      301 tcgcgaacgc gatttcggcg gcgcggagcg ccggcgcggc gccgggcttc cgcgcgatgg
      361 aacgctcggt gagcacgttt gccgtcgatc cgatccgccg gggcggcgcg acgggttgtg
      421 cgttgacgtt cgcagccaac gacgtcgtcg tgtacgccga cgatccgttt gcggcgcgtt
      481 tcgccacggc gtcgctgggt tacacgctgt tcacccccga ggcggtgcgt ctgcggggcc
      541 ggaccatcgc gctccagtcg ctgaagtccg cgttcgtcaa ttcggacgtg cggacgatcg
      601 ggggccgttc ggttcgcgtc acgcgcgagt tcgacttgac gcgcgagctg cggccgttcc
      661 gttacgtcga gcagatgttc gaggttccgt gcaacctgat tgcggacaac ccgctgctga
      721 tctaccgttt cgattttgcc gccgcgcaag ccgccgcggc gcgtacgccg ctgctgcagc
      781 gtctaaacgt ttttttgcaa ggaaaccccg cggtccgcgt cgatacaggg cggtaccatg
      841 cctgacgctc ttccgagacc ggccggcgtc gcggttgtcc catgcttcaa cgaaggtagc
      901 aaccccatcg ggatctctcg cgcgttgctg gagattcccg atcttacggt cgcgttcgtc
      961 gacgatgcga gcgacgcgcc aagccgcgag gtgctggatt cgctggccgg tcgcgacgcg
     1021 cgcgtgcgcg tcgtgcgcaa cg
//
LOCUS       DASUCJ010000077         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1315098, whole genome shotgun sequence.
ACCESSION   DASUCJ010000077 DASUCJ010000000
VERSION     DASUCJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315098"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFU90_00855"
     CDS             complement(<1..442)
                     /locus_tag="VFU90_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEU5478342.1"
                     /translation="MRKAREEGDAGVSAFAAQAVGFLVAVAVVPAAVKALADWASHAL
                     NEVLAAPTHDALRFDTVGIATAVVSLSLPVLLAAGLASGVTAVVQSGGIVATKRMTPR
                     LDRLNPVAGARNLLSPARLFAVARALVAAAIVGWLAYAALADHLV"
     gene            541..>1092
                     /locus_tag="VFU90_00860"
     CDS             541..>1092
                     /locus_tag="VFU90_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5478343.1"
                     /translation="MRFAIIAAGVLAASAVVLSGNTARADATPTGVEVGLRAGYAIPL
                     GDAAGGNPSTSLSNIYSGMIPIQVDAGYRFTPNMMVGAYFAYGITMLNTGNGSLLQGC
                     SQSGVSCSGSNLMFGAQFHYHLMPDQTIDPWAGIGIGYEIASISESAGGQSAGESFSG
                     FQFVNLQVGGDYKVMPNLGVGPFV"
BASE COUNT          178 a          373 c          365 g          176 t
ORIGIN      
        1 ctaccaagtg gtcggcgagc gccgcgtacg cgagccaccc cacgatggcc gccgccacga
       61 gcgcgcgcgc gacggcgaag agcctcgccg gcgagagcag attgcgtgca cccgcgactg
      121 gattcagccg gtcgaggcgc ggcgtcatac gcttcgtcgc gacgattcct cccgactgca
      181 ccaccgccgt cacgcccgac gcgagccccg cggccagcag caccgggaga gagaggctca
      241 cgacggcggt cgcgatgccg acggtgtcga aacgcagtgc gtcgtgcgtc ggcgccgcga
      301 gcacttcgtt aagtgcgtgc gacgcccagt cggcgagcgc cttcaccgcc gcgggcacca
      361 cggcgactgc gacgaggaag cccacggcct gcgctgcgaa cgcgcttacg ccggcgtcgc
      421 cctcttcgcg cgccttgcgc aattttcgtg gtgtggggtc ctccgttttc tcgcccatat
      481 gcgaggccat tgaatagcat cgcaatcatg cgcgtcctac gcgcattcaa ggagggtctg
      541 atgcgattcg caatcatcgc tgcgggtgtg ctcgcggcat ctgcagtcgt tctctcaggg
      601 aacacggcgc gcgccgacgc aacgccgacc ggagtcgagg tgggtctgcg cgcgggatac
      661 gccatcccgc tcggcgatgc ggcgggtggg aatccgtcga cgtccctcag caacatctac
      721 tccgggatga tccccatcca ggtcgacgcc gggtaccgct tcaccccgaa catgatggtc
      781 ggcgcgtact tcgcgtacgg catcacgatg ctcaacacgg gcaacggctc cctgctccag
      841 ggctgcagcc agagcggcgt gagttgctcg ggtagcaact tgatgttcgg cgcgcagttc
      901 cattaccacc tgatgccgga ccagacgatc gacccgtggg cgggcatcgg catcggctac
      961 gagatcgcga gcatcagcga gtcggcgggc gggcagtcgg ccggcgagtc gttcagcggc
     1021 ttccagttcg tcaacctgca ggtcggcggc gactacaagg tgatgccgaa cctcggcgtc
     1081 gggccgttcg tc
//
LOCUS       DASUCJ010000078         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_245411, whole genome shotgun sequence.
ACCESSION   DASUCJ010000078 DASUCJ010000000
VERSION     DASUCJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245411"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1075)
                     /locus_tag="VFU90_00865"
     CDS             complement(284..1075)
                     /locus_tag="VFU90_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478344.1"
                     /translation="MTGLLAKSSAALLAAVLLGTATAVVAQVAEPAVTWNGITLGGAA
                     SSLRSSIGDPLRISLFNDGTRRIARYWVPGMDTTYFLIIEEHGYVVGFDGFSEPAPTA
                     VLENVPPDPTGVRVGDTLEAVKTKHPDFRAGVDDDGAPYLIGKASPTVAVVYSFEGGR
                     VSRFQWGTKLPADRPELSPLSEPSGASPSNAILDVQKNEADGTAWEYRYLAFQPCADG
                     SRWQLKKQALVNDRGRSYDVLHVVCPATKAERDFFFDITSYFGKL"
     gene            complement(1149..1961)
                     /locus_tag="VFU90_00870"
     CDS             complement(1149..1961)
                     /locus_tag="VFU90_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478345.1"
                     /translation="MLDTRATGEAIERAEIRGRRECVAMARRLAPQLNAEIVEIAGGV
                     APFVGAGSPLSEAAGLGMFAEVSARDVDRLTDFYAERATPPRVAVTPLASLDLFGELV
                     RAGYRPVEHQNVLACEIADIDCERDERIVESQDPWAWGRASAAGFMDREPTGDELLTG
                     AIIAAIPSVTALELRTGGTVCATGAMDVQGEYAALFAASTMPDARGRGFQTAMIRDRV
                     ARARRRGARVAHAGAGVASQSERNFRRLGFAVLYTRTVWEKPLIPPQRRATA"
     gene            complement(1973..2461)
                     /locus_tag="VFU90_00875"
     CDS             complement(1973..2461)
                     /locus_tag="VFU90_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5478346.1"
                     /translation="MRLVRMLALGIAATLVFAACAKGSNSSNAESTGAAEATSAAATM
                     AASSAAPMQAAAGSPGEKLYQANCSSCHQADGKGVAGSFPPLAGNPLVIGDPKVVIHA
                     VKFGLTGKIVVQTQTFNGIMPPWSSQLSDAQTASVVTYIRSSWGNKAPAVSAAQVSAV
                     TK"
     gene            complement(2530..3000)
                     /gene="msrB"
                     /locus_tag="VFU90_00880"
     CDS             complement(2530..3000)
                     /gene="msrB"
                     /locus_tag="VFU90_00880"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5478347.1"
                     /translation="MKRSGFVAGLAALAAAEPALVRAAAPKTYPVTHTDAQWRALLGP
                     ERYAILRDGGTEPANSSPLLGETRPGTYRCAGCSLALFSSKTKYDSGDGWPSFWDVLP
                     NAVLKQADYQLIEARTEVHCRQCGGHLGHLFDDGPPPTHLRYCIDGLALRFVPR"
     gene            complement(2997..3491)
                     /gene="msrA"
                     /locus_tag="VFU90_00885"
     CDS             complement(2997..3491)
                     /gene="msrA"
                     /locus_tag="VFU90_00885"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5478348.1"
                     /translation="MSQTTQTATFAAGCFWGVEAAFRQIPGVLDAVSGYTGGTTERPT
                     YREVCGHRTGHAEAVEVTFDPQRVTYEQLLGAFWSIHDPTQLNRQGPDVGDQYRSAIF
                     THSPEQMRAALASRDAERLKHDRPVVTQIVEAPAFWPAEEYHQRYFEKNGGAACHVTP
                     VVPS"
     gene            complement(3536..4975)
                     /gene="dacB"
                     /locus_tag="VFU90_00890"
     CDS             complement(3536..4975)
                     /gene="dacB"
                     /locus_tag="VFU90_00890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU5478349.1"
                     /translation="MRLAAAFVAASFCASIAAARASPPNLTAAIDAAVRTRTLAGAHV
                     GILAVDATGGATLYARDPDGDFVPASTLKLIVGSAALARLGPDFSFITTVDASGPVAG
                     GILHGDLYLRGGGDVQLTPGDLDAAAAAVAAAGIHQVAGALVADTTRYDAARYPPGWE
                     IDDVPYGYAAVPGALGLDLNVAHLRVRPGDAPGAPASLDVDPASSTFRIENASITGPA
                     GSDDTTDLARPWDRPDTIRVTGSYPMGAPPSDDLEPALPDPAAYAADIFQRALAARGV
                     TVRDAIRYGATPADATVVWTHRSKPLPAILRDCWPPSVNVIAEQLLEELGAGSAASAP
                     GDVRARGLAAELAWLQTLGVNAGAVTLVDGSGLSSYDRITPRVLVAALQADWRGPQRA
                     AVLAALPVAGVSGTLQTKFANPPLRGNVYAKTGSMNHARLLAGYLRAKGGATVVFALM
                     VNDWMDDSPHAESALDAFRATVLTALASE"
BASE COUNT          835 a         1679 c         1748 g          830 t
ORIGIN      
        1 accgagggca acggacattg aggtctttat tgactctgcg gggacagtcg tcgatacgag
       61 aatatggagt tcgagcggtg ttgacggtct tgacgaagcc gctcggggtg cggctcaaat
      121 ctctacatac aaaccggccg cgttcctatg tacgccggtt gtcagctacg gtatttttag
      181 attcgaatat cagccgtagt aagggcgacg cggcgttgat gggcgttgcc ccgcacgagc
      241 tgcgctctcc gtcggaggct gcgggcgccg gtttgagaag cggctaaagt tttccgaagt
      301 agctcgtgat gtcgaaaaag aaatcgcgtt ccgccttcgt ggccgggcat acgacgtgaa
      361 gtacgtcgta ggatcgtccg cgatcgttga cgagcgcttg tttcttgagc tgccatcgcg
      421 agccgtcggc gcagggttga aaggcgaggt agcgatactc ccacgcggtg ccgtcggctt
      481 cgttcttctg aacgtcgagg atggcgtttg aaggagaagc tccggaaggt tcgctgaggg
      541 gcgaaagttc gggccgatcc gccggcagtt tcgtgcccca ctgaaaccgc gaaacgcggc
      601 cgccctcgaa cgagtacacc accgccacgg tcggcgaagc cttcccgatg agatacggcg
      661 cgccgtcgtc gtcgacgccg gcgcgaaagt cggggtgctt agtcttcacc gcctccagcg
      721 tatcgccgac gcgcacgccc gtaggatccg gcgggacgtt ctcgagtacc gccgtcggag
      781 cgggctcgga gaatccgtcg aaaccgacga cgtaaccgtg ttcctcgatg atcaagaagt
      841 aggtggtgtc cattcccggc acccagtagc gagcgatcct tcgcgtgccg tcgttgaaaa
      901 gcgaaatccg tagcggatcg ccgatcgacg aacgcaggct cgaagccgct cctccgagcg
      961 tgattccgtt ccaggttacg gccggttcgg cgacctgcgc cacgacggcc gtcgccgtgc
     1021 ccagcagaac ggccgcgagc agagcggcac tcgatttcgc cagcaaaccg gtcatgcacg
     1081 gaatacgtta ggcaaagccc gagtgcgctc cgcttcgcct aagataggcg cgctcatccg
     1141 ggaaaacctc aagcagtagc gcgccgctgc ggcgggatca gcggcttctc ccagacggtg
     1201 cgcgtgtaga gcaccgcaaa gccgagccgg cgaaagttgc gctccgactg ggacgcgacg
     1261 ccggcgccgg catgcgcgac gcgcgcgccg cgccggcgcg ctcgcgcaac ccggtcgcgg
     1321 atcatcgcgg tttgaaaacc gcgtccgcgc gcatccggca tcgtcgacgc ggcaaatagc
     1381 gccgcatact ccccttggac gtccatcgca ccggtggcgc acacggtgcc gccggttcgc
     1441 agctcgagcg cggttacgct cgggatcgcg gcgatgatcg cgccggtcag gagttcgtcg
     1501 ccggtcggtt cccgatccat gaaaccggcc gccgaggccc gtccccacgc ccagggatcc
     1561 tgactttcga cgatccgctc gtcgcgctcg cagtcgatat cggcgatttc gcaggcgagc
     1621 acgttttggt gctcgaccgg ccggtacccc gcgcgtacca attcgccgaa caaatcgagc
     1681 gatgcgagcg gcgttaccgc gacgcgcggc ggcgttgcgc gctcggcgta gaaatcggtg
     1741 agccggtcga cgtcgcgcgc cgacacttcg gcaaacatgc cgaggcccgc ggcttccgat
     1801 agcggcgaac ccgcccccac gaagggcgcc acgccgccgg cgatctcgac gatttcggcg
     1861 ttcagctgcg gcgccaagcg gcgcgccatc gccacgcact cgcggcggcc gcgtatctcg
     1921 gcacgctcga tcgcctcgcc ggtcgcgcgc gtatcgagca acggatgcgg cgttacttcg
     1981 tgacggcgct tacctgcgcc gcgctcaccg cgggcgcctt gttcccccac gacgagcgga
     2041 tatacgtcac gacgctcgcg gtctgggcgt cggagagctg cgagctccac ggcggcatga
     2101 tgccgttgaa ggtctgcgtc tgcaccacga tcttgccggt caagccgaac ttcacggcgt
     2161 gaatcacaac cttcggatcg ccgatcacca gcgggttacc ggcgagcggc ggaaagctgc
     2221 cggctacgcc tttgccgtcg gcttggtggc agctggagca gttcgcttgg tacagttttt
     2281 cgccgggcga tccggcggcg gcttgcatcg gtgccgccga gcttgcggcc atcgtggcgg
     2341 ccgcggacgt cgcttcggcc gcaccggtcg actcggcgtt cgaggagttc gagcctttcg
     2401 cgcaagccgc aaaaaccagc gtcgcggcga ttccgagcgc gagcatcctc accaatcgca
     2461 tcgtctgctt cctccgtcgt cagagcgggc gctttagacc gacggccgcg gattcctcgt
     2521 ttcgcgcgtt tagcgcggca cgaagcgcag cgcgagtccg tcgatgcagt agcgcagatg
     2581 cgtcggcggc ggaccgtcgt cgaacagatg gccgagatgc ccgccgcact gacggcagtg
     2641 cacttccgtg cgcgcctcga tcagctgata gtcggcctgc ttcaagacgg cgttcggcag
     2701 aacgtcccaa aacgacggcc agccgtcgcc gctgtcgtac ttcgtcttcg acgaaaacaa
     2761 cgccaggctg cagccggcgc aacgatacgt tccggggcgc gtctcgccga gtaacgggct
     2821 cgagttggca ggctcggttc cgccgtcacg caggatcgcg tagcgttccg gccccagcag
     2881 cgcgcgccac tgcgcgtcgg tatgcgtcac cggatacgtt ttcggcgccg ccgcgcggac
     2941 cagagcgggc tccgctgccg cgagcgccgc caagccggcg acgaaacccg agcgtttcac
     3001 gatgggacga ccggcgtaac gtgacacgcg gcgccgccgt ttttttcgaa gtaccgctgg
     3061 tgatattctt cggccggcca gaacgccggc gcttcgacga tttgggtgac gacggggcga
     3121 tcgtgtttga gtcgctcggc gtcgcgcgag gcgagcgcgg cgcgcatctg ttcgggcgag
     3181 tgcgtaaaga tcgccgagcg gtactggtcg ccgacgtcgg gaccttggcg gttgagctgc
     3241 gtcgggtcgt gaatgctcca gaacgcgccg agcagctgct cgtaggtcac gcgctggggg
     3301 tcgaaggtga cctcgaccgc ttcggcgtgt ccggtgcgat gtccgcagac ctcgcgatag
     3361 gtcggccgct cggtcgttcc gccggtgtag cccgagactg catccagcac tccgggaatc
     3421 tgccgaaagg cggcttcgac gccccaaaag cagccggcgg cgaaggtggc ggtttgcgtc
     3481 gtttgactca tacacctaga gaacgcccgg cgagcgtttc cggatgcgtt ggctgctact
     3541 cgcttgccag cgccgtcagg acggtggccc gaaaggcatc gagtgcggac tcggcgtgcg
     3601 gcgagtcgtc catccagtcg ttgaccatga gcgcgaaaac gacggtcgcg ccgcccttgg
     3661 cgcgcaggta acccgccagc agacgagcgt ggttcatgct tccggttttt gcgtaaacgt
     3721 tgccgcgcag cggcgggttg gcgaacttcg tttgcagcgt tccggaaacg ccggccaccg
     3781 gcagcgcggc gaggacggcg gcgcgctgcg gcccgcgcca atcggcttgc agcgccgcga
     3841 ccagcacgcg cggcgtaatg cggtcgtacg acgacaatcc ggagccgtcg acgagcgtta
     3901 ccgcgccggc gttcacgccg agcgtttgca gccacgccaa ctcggcggcg agtccgcgcg
     3961 cgcggacgtc gcctggtgcg gaagccgccg atccggcacc cagctcttcc agcagctgct
     4021 cggcaatgac gttcacgctc ggcggccagc agtcgcgcag aatcgcggga agcggtttgg
     4081 aacggtgcgt ccagacgacg gttgcatcgg ccggcgtcgc gccgtagcga atggcgtcgc
     4141 gcaccgtcac gccccgcgcg gccagcgcac gttggaagat atcggcggcg taggccgccg
     4201 gatccggaag cgccggctcg agatcgtccg acggcggcgc gcccatcgga taactgccgg
     4261 tgacgcggat cgtatccgga cggtcccacg gccgcgcgag atcggtagtg tcgtcgctgc
     4321 cggcgggccc ggttatcgac gcgttttcaa tccgaaacgt gctgctcgcc ggatcgacgt
     4381 cgagcgaggc cggcgcgccg ggcgcgtcgc ccgggcgcac gcgcaggtgc gcgacgttga
     4441 gatcgagtcc gagcgcaccg ggcaccgcgg cgtagccgta gggaacgtcg tcgatttccc
     4501 agccgggcgg atagcgcgct gcgtcgtaac gcgtggtgtc ggcgaccagc gcccccgcga
     4561 cctggtgaat gccggccgcc gcgacggcgg ccgcggcggc atcgagatcg cccggcgtca
     4621 gctggacgtc gccgccgccg cgtaagtaca aatcgccgtg caagattccg ccggcgactg
     4681 gtccgctcgc atcgaccgtc gtgataaacg aaaagtccgg cccgaggcgg gccagcgcgg
     4741 ccgagccgac gatcagtttg agcgtcgacg cgggaacgaa atcgccgtcc ggatcgcgcg
     4801 cgtacagcgt cgcaccgccg gtcgcatcga ccgcaaggat gccgacgtgc gcgccggcta
     4861 gcgttcgcgt gcgcacggcg gcgtcgatgg cagcggtgag attcgggggc gacgctcgcg
     4921 cggccgcgat cgacgcgcag aacgacgcgg cgacgaacgc ggccgcgaga cgcatcagaa
     4981 ctgttccgcg aaccgatcga tttgcgattg gtcgacgccg gccgcgagca tctgcaccgc
     5041 cagctggtcg cgatcgcgcg gcggcgtcac gaacatgaac ggtgcggact cg
//
LOCUS       DASUCJ010000079         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_719194, whole genome shotgun sequence.
ACCESSION   DASUCJ010000079 DASUCJ010000000
VERSION     DASUCJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719194"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1175
                     /locus_tag="VFU90_00895"
     CDS             18..>1175
                     /locus_tag="VFU90_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5478350.1"
                     /translation="MLWQHDLCDAGSTTFGASLALDPSGDVLVAGSFFQSAYIAKLSG
                     ATGDKIWDDVIAPSNAQRTALSIGASSNGDVRVATWQNDTITIFGYGSDGTLQFQREL
                     TNSLGSAKLRGFAVDSAGFSVVSEFDTDGNNQTAGRISRIDNFGSFVWQVQGAGGHVA
                     TDSAGNVIAGGINAVIEYDAATGTALWTVAVPATAVIADAQGNVFATGTWGVQPDDDI
                     VTSKIGSDGTILWSHAYVDPGHSGNGASIALDASGNPVATGFGSASSGSATVQTTLKY
                     STNDGHVLWTATDQDSLGGSFPYAVNAGSGGAATVGSIFNASTGGLGGIYAIRYADGG
                     TTTKVDPVLALASSANPSTAGDSVTFTATVTGSAGTPSGSVTFLDGSTAICS"
BASE COUNT          188 a          419 c          394 g          174 t
ORIGIN      
        1 cttccggcga cgggcacgtg ctctggcagc atgacctgtg cgacgcgggc agcacgacgt
       61 tcggagcgtc cctcgcgctc gatccctcgg gcgacgtgct ggtcgcgggc tcgttcttcc
      121 agagcgcgta catcgcgaag ctttcgggcg cgacgggcga caagatctgg gacgacgtga
      181 tcgctcccag caacgcgcag cgcacggccc tctcgatcgg cgcatcgtcg aacggcgacg
      241 tccgcgtggc cacctggcag aacgacacca tcacgatctt cggctacggc tccgacggca
      301 cgctccagtt ccagcgcgag ctgaccaaca gcctcggctc cgcgaagctg cgcggcttcg
      361 ccgtcgactc ggccggcttc tccgtcgtga gcgagttcga caccgacggc aacaaccaaa
      421 ccgcgggccg catctcgcgc atcgacaact tcggctcctt cgtgtggcag gtgcaggggg
      481 cggggggcca tgtcgccacc gattccgcgg gcaacgtcat cgccggcggc atcaacgcgg
      541 tgatcgagta cgacgcggcg accggcaccg cgctgtggac ggtggctgtt cccgcaaccg
      601 ccgtcattgc ggacgcgcag gggaacgtct tcgcgaccgg cacgtggggc gtgcagcccg
      661 acgacgacat cgtcacgtcg aagatcggat cggacggcac gatcctctgg agccacgcgt
      721 acgtcgatcc ggggcactcc ggcaacggcg cgtcgatcgc gctcgatgct tcgggcaacc
      781 cggtcgcgac cggcttcgga tccgcctcca gcggcagcgc aacggtgcag acgacgctca
      841 agtactccac gaacgacggc cacgtgctat ggacggcgac cgaccaggat tcgctgggcg
      901 gctccttccc atacgcggtg aacgcgggga gcggcggcgc cgcgaccgtg ggctcgatct
      961 tcaacgcgtc caccggcggg ctcggcggca tctatgcgat ccggtacgcg gacggcggca
     1021 cgacgaccaa ggtcgatccg gtgctcgcgc tcgcctcctc cgccaatccc tcgaccgctg
     1081 gcgatagcgt caccttcacc gcgacggtga caggcagcgc cggcacgccg agtgggagcg
     1141 tgacgttcct cgacggctcg acggcgatct gctcc
//
LOCUS       DASUCJ010000080         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_596914, whole genome shotgun sequence.
ACCESSION   DASUCJ010000080 DASUCJ010000000
VERSION     DASUCJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596914"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFU90_00900"
     CDS             <1..776
                     /locus_tag="VFU90_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEU5478351.1"
                     /translation="SGTMPAAFVLPVAPEDALRTTGDAFVDELYASAPRHGATLIAAT
                     FPRWMIDANRGRDDIDLEMIEDDWPHPVNPTRKTQSGMGLLRRLALPGVPMYGRKFSV
                     EEVETLLRDYYDPYHAAVKTTLDRTHARFGAVWHIDCHSMKSQGNAMNDDAGRMRPDF
                     VVSDRDGTTAGEGFTEHVADVLRGQDYDVRVNDPYKGAELIRAYGNPSKRRHSIQIEI
                     NRRLYMDEDAVVKHDGFGRLAQGLDRMLDRVAAFVRARL"
     gene            1081..1404
                     /locus_tag="VFU90_00905"
     CDS             1081..1404
                     /locus_tag="VFU90_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478352.1"
                     /translation="MNKLLIALIAGAFAATAWAQGAAPAPAAAPAPAAATAPAKAAEP
                     AKAEPAKADSMKAENGMKHEKKATKHTKKKAKKHAKKEAKEEAPPKEGAAKEGAAKEG
                     TAPAK"
     gene            1528..>1729
                     /locus_tag="VFU90_00910"
     CDS             1528..>1729
                     /locus_tag="VFU90_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478353.1"
                     /translation="MIDTQTIMQAAPESTAAETSGKAGPSLAPDGPERTPTAGRQPAP
                     ARQQSPGRPLTLTRVTAARARPI"
BASE COUNT          341 a          554 c          571 g          263 t
ORIGIN      
        1 acagcggcac tatgccggcg gcgtttgtcc tgcccgtcgc accggaagat gcgttgcgca
       61 cgaccggcga cgcattcgtc gacgagttgt atgcgagtgc gccgcgccat ggcgcaacgc
      121 tcatcgccgc gacgtttcca cgctggatga tcgacgcgaa tcgcggtcgc gacgatatcg
      181 atctcgagat gatcgaggac gactggccgc atccggtgaa ccccacgcgc aagacgcaaa
      241 gcggcatggg attgctgcgt cgcctcgcgc ttcccggcgt gccgatgtat ggacggaaat
      301 tcagcgtgga ggaggtcgag acgttgcttc gcgattatta cgacccctat cacgcggcgg
      361 tcaagaccac gctcgatcgt acgcacgcac gttttggtgc cgtctggcac atcgactgtc
      421 actcaatgaa atcgcagggg aatgcgatga acgacgacgc aggcaggatg cggcccgatt
      481 tcgtcgtcag cgatcgcgac ggaacgaccg cgggcgaggg cttcaccgag cacgtcgccg
      541 acgtacttcg cgggcaggac tacgacgtgc gcgtcaacga cccgtacaag ggcgccgagc
      601 tgatccgcgc ctatgggaat ccgtcgaagc ggcgtcatag catccagatc gagatcaatc
      661 gccgcctcta catggacgag gatgccgtcg tgaagcacga cggcttcggg cgtctcgcgc
      721 agggtctcga ccgaatgttg gatcgagtgg ccgcttttgt tcgcgcgcga ctttgaccgg
      781 cacacgcagg agcgtccggc gggtcgtcgg tcggccgccg gctgggatac ccgctgagac
      841 ggggcccggc atcgatttac gctgcgacgc ggaaatcatg gcattttcga ggctgactgt
      901 cgtcggagga cgacagaaat acagtgtgca aatccttgat tgcggtgaag ctttgccgtc
      961 gatccggtcg acgcatcggc aaggccgctg tcaacgggga catgttcggt cgcgttaatg
     1021 acaccgatgc cgatttccgg cgtcgaaacc cactctcgac agtcgaaaag gactttggac
     1081 atgaacaaat tgctgatcgc cctgattgcc ggcgcctttg ccgccactgc atgggcccag
     1141 ggcgcggcac ctgcgcccgc ggctgctccc gcacccgctg cggcgaccgc tccggcgaag
     1201 gccgccgagc ccgcgaaggc agagccggcg aaggccgact cgatgaaggc cgaaaacggc
     1261 atgaagcacg aaaagaaggc caccaagcac accaagaaga aggccaagaa gcacgccaag
     1321 aaggaagcga aggaggaagc cccccccaag gaaggcgcgg ccaaggaagg cgcggcgaag
     1381 gaaggcacgg cgcccgccaa gtaagctccg cgtctttcgc gactgccccg gtggcgcgtt
     1441 gccccggggc agttcgtttt cgggggtcgg tccgcgagcg cgagcgctgc ggcctgactc
     1501 ttgcattgcg aaccccggag gttcgcaatg atcgatacgc agaccatcat gcaggcagcg
     1561 cccgaatcga ccgccgccga aacgagcggc aaagcaggac cgtcgctcgc gccggacggc
     1621 cccgaacgta cgccgactgc gggtcgccag ccggcgcccg cccgtcagca gtcgccgggc
     1681 cgacccttga cgctcacccg ggtgacggcg gcgcgcgcgc gcccgatcc
//
LOCUS       DASUCJ010000081         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_291244, whole genome shotgun sequence.
ACCESSION   DASUCJ010000081 DASUCJ010000000
VERSION     DASUCJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291244"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="ettA"
                     /locus_tag="VFU90_00915"
     CDS             <1..1049
                     /gene="ettA"
                     /locus_tag="VFU90_00915"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU5478354.1"
                     /translation="PGTVVGVTHDRYFLDNACQWILELDRGYGIPWEGNYSSWLEQKQ
                     ERLAQEEAAESARQKALRRELEWVRAGVKGRGSKNKSRIARFEELSSYEYQRRNETRE
                     IFIPVAERLGDRVIELENVTKAYGDRLLMENVSLTIPAGAIVGIIGPNGAGKTTLFRM
                     IAGRERPDAGEIRLGPTVKLGIVEQSRESLDDAKTVFDDVSGGLDNLTVGKFTMPSRA
                     YLGRFNFKGGDQQKLVGTLSGGERGRLHLAKTLLAGANVLLLDEPSNDLDVETLRALE
                     DALSEFAGTVLVVSHDRWFLDRIATHILAFEGDSRVAFFEGNYREYEADKKTRLGEEG
                     AAPHRIRYRPLTLR"
     gene            1046..1852
                     /locus_tag="VFU90_00920"
     CDS             1046..1852
                     /locus_tag="VFU90_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5478355.1"
                     /translation="MTPGIALAAMLAAAATNGAPRAAHVTVVVMENRAYGGIVGNADA
                     PYFNDRLLPQGALLENSHAVAHPSEPNYVALFSGSTQGLTSDACPVSYASPNLASNLA
                     RAGKTFALYAESLPSDGYRGCWGGGYARKHDPAADFTNVPASANRVYRGVPASVPDVM
                     WIVPNVCHDMHDCSTAAGDAWLAGNLPSLIAWNARNDGLLILTWDEAEPDADGSNRIP
                     TLLAGPMVRPGARSAQYVDHYSVLRTIEGIFALGCIARECGVTPIEGVWR"
     gene            complement(1824..>3203)
                     /locus_tag="VFU90_00925"
     CDS             complement(1824..>3203)
                     /locus_tag="VFU90_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478356.1"
                     /translation="ASDAQWVIEGYTLVLGALMLLCGALGDRYGRKRVFTIGVAIFAI
                     GSILCGLAPSMPALIGARVLQGIGGTMLAPASLALLGASFEGADRGRAIGLWSGLTAV
                     AGAIGPVAGGTIVDHFGWRWIFFINVPLAAAVVAIALRHMRESRDESASGPLDVAGSV
                     SITAGLGGIVYAFVVSGLDGWTLQPLAALVAGIAAFAVFLAIERRTVNPILPFGLFAR
                     RAFSGINVMTFLLYGALSGLFYFLPFVMILVNGYSATVTGLAMLPFLVLIVALSRYSG
                     ALVYRAGRRAVLVAGPAITALGFLAFALLPDLHYWSGVFPSVVLVGLGMGFTVAPLTT
                     TLIDSVPEHNVGLASGINNAVSRVAALLAIAILGALVAAVFGARLEGRMDRAGLSSVQ
                     RRDVNAQRSAMAGARLADARERAVVRAAYLDGFRAVAATCALLAAGSALAAAATQGGA
                     ASARRPRSA"
BASE COUNT          608 a         1074 c         1066 g          455 t
ORIGIN      
        1 tccccgggac agtggtcggc gtgacgcacg accgctactt cctcgacaac gcctgccagt
       61 ggatcctcga actggatcgc ggctatggca tcccgtggga aggcaactac agctcgtggc
      121 tcgagcaaaa acaggagcgc ctcgcgcagg aagaagcggc cgagtcggcc cggcaaaagg
      181 cgctgcggcg cgaactcgaa tgggtgcgcg ccggcgtgaa gggccgcggc tccaagaaca
      241 agagccggat cgcgcgcttc gaagagcttt ccagctacga gtaccagcgg cgcaacgaaa
      301 cgcgggagat cttcattccg gttgccgaac ggctcggcga tcgcgtgatc gagctcgaga
      361 acgtaaccaa agcgtacggc gaccggctgc tgatggaaaa cgtcagcctg acgattccgg
      421 ccggtgcgat cgtcggcatc atcggcccga acggcgccgg aaaaacgacg ctgtttcgca
      481 tgatcgccgg gcgcgagcgg ccggatgcgg gcgagattcg cttgggaccg acggtcaagc
      541 tcggcatcgt ggagcaaagc cgcgaatcgc tcgacgacgc taagacggtc ttcgacgacg
      601 tcagcggcgg cctcgacaac ctcacggtcg gcaagttcac gatgccgtcg cgcgcctact
      661 taggccggtt caatttcaaa ggcggcgatc agcaaaaact ggtggggacg ctttcgggcg
      721 gcgagcgggg ccggctgcac ctcgcaaaga cgctgctcgc gggcgccaac gtcctgctgc
      781 tcgacgagcc ctccaacgat ctcgacgtcg agacgctgcg cgcgctggaa gatgcgttga
      841 gcgagtttgc gggaaccgtg ctggtcgtca gccacgaccg ctggttcttg gatcggatcg
      901 ctacccacat tcttgccttc gaaggcgact cgcgggtcgc gttcttcgaa gggaactatc
      961 gcgaatatga agccgacaaa aagacgcggc tcggcgaaga gggcgccgct ccccaccgca
     1021 tccgttatcg cccgctgacg ctgcgatgac gccggggatc gcgctcgcgg cgatgctggc
     1081 cgccgcggcg acgaacgggg cgccgcgcgc ggcgcacgtg accgtcgtcg tgatggaaaa
     1141 ccgggcgtac ggcggtatcg tcggcaacgc cgacgccccg tatttcaacg atcgcctgct
     1201 cccgcaggga gcgctgctgg aaaatagcca tgcggtcgcc catccaagcg agccgaacta
     1261 cgtcgcgctg ttttcaggtt cgacgcaagg actgaccagc gacgcgtgcc ccgtgtcgta
     1321 cgcctcgcca aaccttgctt cgaatctcgc tcgcgccggg aagacgttcg cactctatgc
     1381 ggagtcgctg ccgtccgacg gctatcgcgg ctgctggggc ggcggctacg cgcgcaagca
     1441 cgatccggcg gccgacttta ccaacgttcc ggcatcggca aaccgggtgt atcgcggcgt
     1501 gccggcgagc gtacccgacg tcatgtggat cgtgccgaac gtctgtcacg acatgcacga
     1561 ctgttcgacc gccgcgggcg acgcatggct cgccgggaac ctgccttcgc tgatcgcttg
     1621 gaacgcgcgc aacgacgggt tgctgattct cacctgggac gaagccgagc ccgatgccga
     1681 cggcagcaat cgcattccca cgctgctggc cggcccgatg gtccgccccg gcgcgcgcag
     1741 cgcgcagtac gtcgatcact attccgtgct tcgaacgatc gaagggattt ttgcactcgg
     1801 ctgcatcgcg cgcgagtgcg gcgttacgcc gatcgagggc gtctggcgct agccgcgccg
     1861 ccttgggtcg cggcggcagc gagtgccgag ccggccgcga gcagcgcgca ggtcgcggcg
     1921 acggcgcgaa aaccgtcgag gtaggcggcc cgcacgaccg ctcgttcgcg cgcatccgcc
     1981 aaacgtgcgc cggccatcgc gctgcgttgg gcgttgacgt cgcgcctctg aacggagctg
     2041 agtccggcac gatccatccg cccttcgagc cgcgcgccga atacggcggc gaccagcgct
     2101 ccgaggatgg cgatcgccag cagcgccgcg acgcgtgaga ccgcattgtt gattcccgac
     2161 gccagcccga cgttgtgttc gggtaccgag tcgatcagcg tcgtcgtgag cggggccacg
     2221 gtaaagccca tcccgaggcc gaccaggacg acgctcggaa acacgccgct ccaatagtgc
     2281 aagtcgggca agagggcgaa ggcgagaaat ccgagcgccg taatggccgg gccggcgacc
     2341 agcaccgctc gtcgcccggc gcggtagacg agtgcgcccg agtagcgcga cagcgcgacg
     2401 atgagcacca gaaacggcag catcgccaat cccgtgacgg tcgcactgta cccgttgact
     2461 aaaatcatga cgaacggaag gaagtaaaac agtccgctga gcgccccgta cagcaagaac
     2521 gtcatgacgt tgatcccgga gaacgcgcgt cgcgcgaaca agccgaacgg aagaatcgga
     2581 ttgacggtgc gccgctcgat cgccaggaaa acggcgaacg ccgcgatgcc ggcaaccaac
     2641 gcggccaagg gttgcagcgt ccagccgtcg aggccgctga cgacgaatgc gtagacgatg
     2701 ccgccgagcc ccgcggtaat cgagaccgag ccggcaacgt cgagcgggcc gctcgccgat
     2761 tcgtcacggc tctcgcgcat gtgccgcagc gcgatcgcaa cgaccgccgc cgcgagcgga
     2821 acgttgatga agaaaatcca gcgccagccg aaatggtcga cgatcgtgcc gccggcgacc
     2881 gggccgatgg cgcccgcgac ggcggtaagc cccgaccaca ggccgatcgc gcgtccgcga
     2941 tccgcgcctt cgaacgacgc tcccagcagc gcgaggctgg caggcgccag catcgtgccg
     3001 ccgattcctt gcagcacccg agcgccgatc agcgccggca tcgacggcgc caggccgcac
     3061 aggatcgatc cgatcgcaaa gatcgcgacc ccaatcgtga agacgcgctt gcgcccgtac
     3121 cgatcgccga gcgcgccgca gagcagcatc agcgcgccga gcacgagcgt gtacccttcg
     3181 atcacccact gcgcgtcgct cgc
//
LOCUS       DASUCJ010000082         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_291256, whole genome shotgun sequence.
ACCESSION   DASUCJ010000082 DASUCJ010000000
VERSION     DASUCJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291256"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFU90_00930"
     CDS             <1..681
                     /locus_tag="VFU90_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU5478357.1"
                     /translation="YPDLAFSLPVPRGGCARAGSVGVGVMDYSGSNDDRRDSEAIRAR
                     YIDAMTRFVLWLADHGRPVVLLIGDTQDERVIAGVLADVRAQRPQLDQSAVTTAPAQS
                     LGELMRQIEGVETVVATRFHTVLCALRLGKPTLAVGYAAKFDALMAEMGLAEFSLPAK
                     ALDFDVMVDRFAELEGKRAYCSERTAARSAANERLLNEQFAELSAALFDRIPAAQRPV
                     LCSGDTHG"
     gene            674..>1013
                     /locus_tag="VFU90_00935"
     CDS             674..>1013
                     /locus_tag="VFU90_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478358.1"
                     /translation="MDDVLRRGADDPAEGRAVRYYAKDVWSDENLKYVEPHFRMLQCA
                     RVVTRLAEGRTPSLLDIGCGPATLRRLIPATVAYHGIDIAIHEQDPNLREADIVKSPI
                     TFRDQKFDIVI"
BASE COUNT          164 a          345 c          341 g          163 t
ORIGIN      
        1 taccccgacc ttgcgttctc attgcccgtt ccccgcggcg gatgcgcacg agcagggagc
       61 gtcggcgtcg gcgtcatgga ctactccggc agcaacgacg accggcggga ttccgaggcc
      121 atccgcgcgc gctacatcga cgcgatgaca cgctttgtgt tgtggctggc cgaccacggc
      181 cggccggtcg tgctgctgat cggtgacacc caggacgagc gcgtcatcgc gggtgtgctg
      241 gccgacgtcc gggcgcagcg gccgcagctc gaccaatccg cggtcaccac ggctccggcg
      301 cagtcgctcg gtgagctcat gcggcagatc gaaggcgtcg agaccgtcgt ggccacgcgc
      361 tttcacaccg tgttgtgcgc gctgaggctc ggcaagccca cgctggcggt cggctacgcc
      421 gccaagttcg acgcgctcat ggccgagatg gggctggcgg agttctcgct gccggcgaag
      481 gcgctcgatt tcgatgtcat ggtcgaccgg ttcgccgagt tggagggcaa gcgcgcgtac
      541 tgcagcgagc ggacagccgc gcgcagcgcc gcgaacgagc ggctactcaa tgagcaattc
      601 gccgagctgt cggccgcgct cttcgaccga atcccggccg cgcaacggcc cgtcctttgc
      661 tctggagaca cccatggatg acgtgctccg taggggtgcc gacgacccgg ccgaagggcg
      721 ggcggtgcgc tactacgcca aagatgtttg gagcgacgag aatctcaagt acgtcgaacc
      781 gcactttcgg atgctccagt gcgcgcgcgt cgtcacgcga ctcgccgagg gccgcacgcc
      841 gtcgctgctc gacatcggtt gcgggccggc gacgctccgg cgcctgatcc ccgcgaccgt
      901 cgcgtatcac ggcatcgaca tcgccatcca tgagcaggac cccaacctga gagaggccga
      961 catcgtcaag tccccgatca cgttccggga ccagaagttc gacatcgtga tcg
//
LOCUS       DASUCJ010000083         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1223555, whole genome shotgun sequence.
ACCESSION   DASUCJ010000083 DASUCJ010000000
VERSION     DASUCJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223555"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFU90_00940"
     CDS             <1..294
                     /locus_tag="VFU90_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5478359.1"
                     /translation="PTVTLVPKQFQGLPSPIPDSGKVTFTLLGDLTVHGVTRPTNWAV
                     TAYLRNGEVRGTASTGVTFDDFKLDKPKVGSVLSVADSIHLVYDFDLVPASGS"
     gene            339..1775
                     /locus_tag="VFU90_00945"
     CDS             339..1775
                     /locus_tag="VFU90_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5478360.1"
                     /translation="MMLARWAAAGLILAAAASARVRAQGARPALSPAVKDVAAIDTNI
                     VALTHVRVIDGTGAPARADQTVIVRDGRIAQIGAAAAVAVPSGARVLDLTGKSVIPGL
                     VMVHEHLFYPTGPGVYGNVAESFVRLYLAGGVTSMRTGGNMNGYGDIGLARAIARGEK
                     PGPWIDATAPYLEGPGLGLDQIHELTGPDDARRMVDFWADAGATSFKAYMHITRAELG
                     AAIAEAHRRGLKVTGHLCSVTYREAASLGIDNLEHGFFVSTDFVPNKQPDVCPGQNTG
                     LATIATLTPDSARVRDLIKFLVDHHVAITSTLTVFETFTPGRPMPRGLDVLDPILREQ
                     FEQRYAATQRDTTRRFDAIFAHARALEVAFMRAGGLLLSGTDPTGGGGVVPGFSNQRE
                     LELLVESGLTPLEAIKVGTLNGATYLGRAKDVGSIAQGKQADLVVIDGDPSTNIADIE
                     HVEMVFRDGIGYDPQKLIASVKGKVGLF"
     gene            2051..2383
                     /locus_tag="VFU90_00950"
     CDS             2051..2383
                     /locus_tag="VFU90_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5478361.1"
                     /translation="MLDRHGAVCTYCERRVHPDAITLDHVTPRRGQTAYDRRDNLVLA
                     CPDCNALKADKPILAFLIARRDRAVNLLRFGEHLSPMLLDLARQIAGPEGVARAERLS
                     DPDYPYKD"
     gene            2496..2741
                     /locus_tag="VFU90_00955"
     CDS             2496..2741
                     /locus_tag="VFU90_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478362.1"
                     /translation="MIAAILRLLALRPLLGLAILGFPILLLVVVGLVTILAFKFLVFI
                     VLPIVAVVWLVRMFKRSSSTHETGLDGETDAGPDAAS"
BASE COUNT          493 a         1009 c          915 g          500 t
ORIGIN      
        1 cccacggtga cgctggtgcc gaagcagttc cagggtctgc cgtcgcccat tccggattcc
       61 ggcaaggtga cgttcacgtt gctgggcgat ctcaccgtgc acggcgtgac gcggcccacg
      121 aattgggcgg tgacggcgta cctgcgcaat ggcgaagtac gcggcacggc atccaccggc
      181 gtcacgttcg acgacttcaa gctcgacaaa cccaaggtcg gctcggtgtt gagcgtggcg
      241 gacagcatcc acctggtgta cgatttcgat ctggtcccgg cgtcgggctc ctgaccgcgg
      301 aggcgcgccg ggcgccgccg cctaacgaaa ggcacgtcat gatgctcgca cgctgggccg
      361 ccgcgggact gatcctcgcc gcggcggctt ccgctcgtgt acgcgcgcaa ggggcgcggc
      421 cggcgctgtc gccggcggtc aaggacgtcg ccgcgatcga cacgaacatc gtcgccctca
      481 cgcacgtgcg cgtgatcgac ggcacgggtg cgccggcgcg cgccgaccag acggtaatcg
      541 tgcgcgacgg acgcatcgcg cagatcggcg cggcggccgc ggttgccgtg ccgtctggcg
      601 cgcgcgtcct ggatctcacc ggcaaatccg tgatccccgg actggtgatg gtgcacgagc
      661 acctgttcta tcccaccggt cccggcgtct acggaaacgt ggccgagagc ttcgtgcgcc
      721 tctatctcgc cggcggcgtg acgtcgatgc gcaccggcgg caacatgaac ggctacggcg
      781 acatcggtct cgcgcgggcg atcgcacgcg gcgagaagcc tggaccgtgg atcgacgcca
      841 cggcgccgta cctcgaggga cctgggttag gcctcgatca gatacacgag ctcaccggac
      901 cggacgatgc gcgccgcatg gtcgacttct gggccgacgc cggcgcgacg tccttcaagg
      961 cgtacatgca catcacccgc gccgagctcg gcgcggcgat cgccgaagcg catcggcgcg
     1021 ggttgaaggt gaccggccac ttgtgctcgg tcacgtaccg cgaagcagct tcgttaggca
     1081 tcgacaacct ggagcacgga ttcttcgtgt ccacggattt cgtgccgaac aaacaaccgg
     1141 atgtgtgccc ggggcagaac acgggactcg cgaccatcgc caccctcacc cccgactcgg
     1201 cgcgcgtacg cgacctcatc aagtttctcg tggaccatca cgtcgcgatc acgagcacgc
     1261 tcacggtgtt cgaaacgttc acgccgggcc ggcccatgcc gcgcggcctc gacgtgctcg
     1321 acccgatcct gcgggaacag ttcgaacagc gctacgccgc gacgcagcgc gacacgacgc
     1381 ggcgcttcga cgcgatcttt gcgcacgcac gcgccctcga agtcgcattc atgcgcgcgg
     1441 gcggactgct gctgtcgggc accgacccga ccggcggtgg cggcgtcgtc cctgggttct
     1501 cgaaccagcg cgagctcgaa ttgctcgtcg aaagcggttt gaccccgctg gaggccatca
     1561 aggtgggcac gttgaacggc gccacctacc tcggccgcgc caaagacgtc ggttcgatcg
     1621 cccagggcaa acaggcggac ctcgtcgtga tcgacggaga tccgtcgacg aatatcgccg
     1681 acatcgagca cgtcgagatg gtgttccgcg acgggatcgg atacgatccg caaaaactca
     1741 tcgcgtcggt gaagggaaaa gtcgggctgt tctgaccatg acagcccgga tttgacggat
     1801 cgctcggctg aggaagccca actcgccgga ttggtcctgg acgcgcgtga cgccggcgga
     1861 ccttcccacg tcaacacctt gtcgctatgt tacagcgtag tcgcatgttg cattaatcac
     1921 cctcaagccc cttgtaccac tgagtcgcac ccgcccatcc gcgcgcagac gccgcagtcc
     1981 cgcgcgagca caccagcagc acacctcgct gccaacaagt cgtaccgctt acacgaacac
     2041 gcgtcagtgg ctgctcgacc gccatggtgc cgtatgtacg tactgcgagc gacgcgttca
     2101 tcccgatgcg atcacgctcg atcacgtgac gccacgccgc ggccagactg cctatgatcg
     2161 ccgcgacaat ctcgttctcg cctgtcccga ctgcaacgcg ctgaaggcag acaagccgat
     2221 cctcgcgttc ctgatcgcac gccgtgatcg cgcggtgaat ctgctccgct ttggcgagca
     2281 cctgagcccc atgctgctcg accttgcccg gcagatcgcc ggacccgaag gcgttgcgcg
     2341 cgccgagcgc ttgtctgacc cggactatcc gtacaaggac tgaaacgcgc ccgtgccggc
     2401 cgcgacgcga ctcgcgcgtc gaaaaagaag gatccgatcg gaaaccgtcc tgccggttcc
     2461 ccccgtagta tagagaaagc ccatcggact ccgacatgat tgccgccata ctccgcctgc
     2521 tcgcgctgcg accgctactc ggcctcgcca tccttggctt cccgatcctg ctgctcgtgg
     2581 tcgttggcct cgtcacgatt ctggccttca agttcctcgt tttcatcgtg ctgccgatcg
     2641 tggcggtcgt gtggctcgtc cgcatgttca agcggtcgtc gtccacgcat gaaaccggac
     2701 ttgacggtga aaccgatgcc gggccggacg ccgcgtcgtg attgaagggt tgcgcgacgc
     2761 gaaggtgaag atgtgaagct cgcgcgcatt caacgtctgg aacggttcgc ggtcggttcg
     2821 ctcctgttga gcctcgcgct catcgcggcg gaccgcatcc gccaccctcg cggcctgttc
     2881 ttcctcgtgc cgttgccgcg tcaccgaacc ggcgcct
//
LOCUS       DASUCJ010000084         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1193094, whole genome shotgun sequence.
ACCESSION   DASUCJ010000084 DASUCJ010000000
VERSION     DASUCJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193094"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFU90_00960"
     CDS             <1..417
                     /locus_tag="VFU90_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478363.1"
                     /translation="GTVPVHRFVKEIPIVAMGLTVALFAYGWLRDSRAARVCAYAGFA
                     LAGGFSIVLAASGERAASALAPQAASAVFAAHRILAGTALALAMLCLLLAASLYVLEG
                     KPRFYRVAAPALAALSLITFLAMLATAGVGKTMFGP"
     gene            436..864
                     /locus_tag="VFU90_00965"
     CDS             436..864
                     /locus_tag="VFU90_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5478364.1"
                     /translation="MRVVIAIDGSYVALRALRTFLGLAKELAAPPEVHLVSVADYIEM
                     PAGLSKAPPQAPDLLASEAETALAAASELARELGVQVRPVLLHGHVVDAVLEYARQID
                     AGLIVAGTHGRKGFRRAVLGSTCEGLLRHSDVPVLSVRYG"
BASE COUNT          131 a          337 c          365 g          211 t
ORIGIN      
        1 ggtaccgttc cggttcaccg gttcgttaag gagattccga tcgtggcgat gggcctgacg
       61 gtcgcgctct tcgcatacgg ctggcttcgc gattcgcgcg ccgctcgggt ttgtgcctac
      121 gccggctttg cgcttgcagg cggcttttcg atcgtgctgg cggccagcgg cgaacgcgcg
      181 gcatcggcgc tggctccgca agccgcatcg gcggtatttg cggctcaccg catcctcgcg
      241 ggtacggcgc tggcgctcgc gatgctatgc ctcctgctcg cggcgtcgct ctacgtgctc
      301 gaaggaaagc cgcgattcta ccgcgtcgca gcgcccgcgc tggcggcgct ctccctcatc
      361 acattcctag caatgctggc caccgccggc gtcgggaaga cgatgttcgg tccttaagga
      421 gatatggagt tcggcatgcg cgtcgttatc gcgatcgacg gttcttacgt ggcgctgcgg
      481 gcgttgcgga cgtttctcgg attagccaaa gagctggcgg ccccgccgga agtgcatctc
      541 gtgtcggtgg ccgactacat cgagatgccc gcggggcttt ccaaggcgcc gccgcaggcg
      601 cccgatctgc tcgcctcgga ggccgaaacc gcgctggccg cggcgagtga gctcgctcgc
      661 gaactcggcg tgcaggtcag gcccgtactc ttgcacggtc acgtggtcga cgccgttttg
      721 gagtacgcgc gtcagatcga cgcgggtttg atcgtggccg ggacgcacgg gcgcaaggga
      781 tttcggcgcg cggtgctcgg gagcacgtgc gagggtctgc tgcgccattc ggacgttccg
      841 gtgctgtccg tgcgttacgg ctgacgcgtt tgcggcggtt agcgtacttc aactgcttgc
      901 gggcggaccg tcacaccgtc gcgaatcgct tcggccggac gcaggatcac gacgtcgccg
      961 gccgtcgcgc cggcgagcac ctgggcgtac tccgccccga tcgctcctag gcgtaaggtt
     1021 tgccggcggg cctttccgtt gtcg
//
LOCUS       DASUCJ010000085         1158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_230214, whole genome shotgun sequence.
ACCESSION   DASUCJ010000085 DASUCJ010000000
VERSION     DASUCJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230214"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="xrtA"
                     /locus_tag="VFU90_00970"
     CDS             complement(<1..967)
                     /gene="xrtA"
                     /locus_tag="VFU90_00970"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEU5478365.1"
                     /translation="MKRDALPGLGTQVISPEVSESATSAPAASTPDASPLAAPGWPRA
                     LVTLGVVLAVIVAAYFGTVAGMVAIWARSETFAHGFVIVPIALWLVWRMRSRLAQIEP
                     QPSWLPVPVMAVAGFAWLVGEVGAVNALTQFAFVAMLVLAVPATLGLRVARAMLFPLG
                     FLFFAVPFGEFLLPTLMAYTADFTIAAVRLTGVPVLREGLQVFSVPNGRWSVVEACSG
                     VRYLIASVVVGTLFAYLNYRTKRRRLAFVGVSIAVPIVANWLRAYIIVMLGYLSDNKI
                     ATGVDHIIYGWLFFGLVMLIMFWVGSYWREAEVAPTPGAQVPAQRD"
     gene            complement(973..>1158)
                     /locus_tag="VFU90_00975"
     CDS             complement(973..>1158)
                     /locus_tag="VFU90_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478366.1"
                     /translation="FKKNWGFDPAPLAYEYCLLDGSEVPQNNPMNPRFRALIATWRHL
                     PRPLVNAIGPWLARNLG"
BASE COUNT          239 a          396 c          386 g          137 t
ORIGIN      
        1 gatcccgttg cgcgggaacc tgcgcacccg gtgtgggtgc gacctcggcc tcgcgccaat
       61 acgaaccgac ccagaacatg atcagcatca cgagaccgaa gaacagccag ccgtaaatga
      121 tgtggtcgac gccggtcgca atcttgttgt ccgaaaggta gccgagcatc acgatgatgt
      181 acgcgcgaag ccagttcgcg acgatcggca ccgcgatcga tacgccgacg aacgcgagcc
      241 tgcgccgctt cgtccggtaa ttgagatagg cgaacagggt gccgacgacg accgacgcga
      301 tcaggtagcg cacgccgctg caggcttcga ccaccgacca gcgcccgttc ggaaccgaga
      361 acacctgcag gccttcgcgc agtaccggca ccccggtgag gcgcaccgcg gcgatcgtga
      421 agtccgcggt gtacgccatc aacgtcggca gcagaaactc gccgaacggc accgcgaaga
      481 acaggaagcc cagcgggaac agcatcgcgc gcgcgacacg caggccgagg gtcgccggga
      541 ccgcgagcac cagcatcgcg acgaacgcga actgcgtcag cgcgttgacg gcccccacct
      601 cgccgacgag ccacgcgaag cccgcgacgg ccatgacggg aaccggcagc cacgacggct
      661 gcggctcgat ctgggcgagc cgcgagcgca tccgccagac gagccacagg gcgatcggca
      721 cgatgacgaa cccgtgcgcg aacgtctcgg agcgcgccca gatcgcgacc atccccgcga
      781 ccgtgccgaa ataagcggcg acgatgacgg caagcacgac gccgagcgtg acgagcgccc
      841 gcggccagcc cggcgccgcc agcggcgagg cgtcgggcgt cgaagccgcc ggtgccgaag
      901 tggctgactc cgagacctcc ggagaaatga cctgcgtgcc gagaccggga agagcgtcgc
      961 gcttcatcgt cgtcagccga ggttgcgcgc gagccatggc ccgatcgcat tgacgagcgg
     1021 ccgcggcaga tggcgccacg tggcgatcag cgcacggaac ctgggattca tcggattgtt
     1081 ttgcggcacc tcgctcccgt cgagcaggca gtactcgtag gcgagcggcg ccggatcgaa
     1141 accccagttc ttcttgaa
//
LOCUS       DASUCJ010000086         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1009, whole genome shotgun sequence.
ACCESSION   DASUCJ010000086 DASUCJ010000000
VERSION     DASUCJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFU90_00980"
     CDS             <1..288
                     /locus_tag="VFU90_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478367.1"
                     /translation="ANDYIYAFEASRDYNPEPKLGDIKAPLIAVNSEDDQVNPPRLGV
                     MERDMPKVPHGQYVLIPISDKTRGHGTHTLAAIWQSHLADLLGEIKHNAGR"
     gene            300..671
                     /locus_tag="VFU90_00985"
     CDS             300..671
                     /locus_tag="VFU90_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478368.1"
                     /translation="MRPVLLVISDLLFRSRIDDAARHASVPLRVAKSMDQLERHLSKE
                     TPSMAIVDLETDTIDTSAAIRRLRAVPDGESLPILAYAGHTNIDAIKAGREAGAGVVL
                     ARSAFAAQLPAILARAAGAAE"
     gene            763..1044
                     /locus_tag="VFU90_00990"
     CDS             763..1044
                     /locus_tag="VFU90_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478369.1"
                     /translation="MIQDVTTALAPDAVLAEAKRFFPSRNPLYAAFVEKEGAGYVTFR
                     GQGGEELVIGVTPMEGGTRVRGSTYLFDAQISRFFASLDPHPMVEEITG"
     gene            1041..1370
                     /locus_tag="VFU90_00995"
     CDS             1041..1370
                     /locus_tag="VFU90_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478370.1"
                     /translation="MTAPPPFVTTLRARRETVRLGTPNGAAFTLRVELPEQWDVVRIV
                     APPNERVLAVKVAALAVLDPRADVREFVIKINGREVLDEQRSVSDVGARDGSIFLITH
                     RRRRPVR"
     gene            1417..2349
                     /locus_tag="VFU90_01000"
     CDS             1417..2349
                     /locus_tag="VFU90_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5478371.1"
                     /translation="MSVAFISHADCGRHDTGWGHPEHVGRLRAITRALRERPDLFGTI
                     EHVEGRHATAQELALAHDPIYIREVEEIASAGGGALDADTVASEGSWAAATAAAGCVL
                     DGVDMALDGRAVRSFCAVRPPGHHALRDRAMGFCLFGNVAVAAHYARRTHGLDRVLIV
                     DWDVHHGNGTQALVEADHGIHFVSMHQWPWYPGSGAADDHGNGNVWNVPLPAGLPAQQ
                     YVDALEQGIDRATDGFTPDLVLVSAGFDSLRGDPLGGFTLELEDITRLTRHLTDRAAQ
                     WCGGRVVGALEGGYAPDRVGEACVTMLAELAQTR"
     gene            2609..>3144
                     /locus_tag="VFU90_01005"
     CDS             2609..>3144
                     /locus_tag="VFU90_01005"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEU5478372.1"
                     /translation="MRLFGVMSVARDSDGGHPGEASLVRTRDLAALVKQVPDSRRDLD
                     DADIDLHQRVVESAFERGGVLPAPCGTVFRNAEQLRRWMEQNYIALSEGLQFVAGRSE
                     ARVHIARRPTTEAEASTPATSAVLTDCFRTLRRVSSAAMTLPPVHHPEHALFSAAFLL
                     DRERWAEFETTVRSLAHR"
BASE COUNT          508 a         1126 c         1001 g          509 t
ORIGIN      
        1 gccaacgact acatctacgc gttcgaggcg tcgcgcgatt acaacccgga gcccaagctc
       61 ggcgacatca aagcgccgct cattgccgtc aactccgaag acgatcaggt gaatccgccc
      121 aggttaggcg tcatggaacg agacatgccc aaggtgccgc acggacagta cgtgctcatc
      181 ccgatcagcg acaagacgcg cggccacggc acgcacaccc tggcggcgat ctggcagagc
      241 cacctggcgg atctgctcgg cgagatcaag cacaacgccg gccggtagcc cgtcgaccca
      301 tgcgcccggt tctgttagtc atctccgatc tgctgtttcg ctcgcgcatc gatgatgccg
      361 cgcgccacgc gtctgtcccg ctgcgcgtcg caaaatcgat ggatcagctc gagcggcact
      421 tgagcaaaga gacgccatcg atggcgatcg tggacctcga aaccgacacg atcgatacgt
      481 cggccgccat ccgccggctg cgcgccgtac cggatggcga atctctgccc attcttgcct
      541 acgccggcca caccaacatc gacgcgatca aggctggccg cgaggccggc gccggcgtcg
      601 tgctcgcgcg atcggcgttc gccgcgcaac tgccggcgat tcttgcgcgc gctgccggag
      661 ctgccgaata agccgagtcg tccctgccga tgtccgacgc ggcataatag attcatgttg
      721 tagtcgagac agctgtcccg cgaccaccca ggattcggcc acatgattca ggacgtcacg
      781 accgctcttg cccccgacgc ggtgctcgcc gaggccaagc ggtttttccc gtcgcgcaat
      841 ccgctgtacg ccgcgttcgt cgagaaggaa ggcgccggtt acgtcacctt ccgcgggcag
      901 ggcggagaag agctcgtgat cggcgtcacg cccatggagg gcggcacgcg ggtgcgcggg
      961 tccacgtatc tcttcgacgc gcagatctcc cgcttcttcg cgtcgctcga cccgcacccg
     1021 atggtcgagg agatcaccgg atgacggcgc cgccgccctt tgtcacgacg ctgcgcgccc
     1081 gccgcgagac cgtgcggttg ggcacgccta acggagcggc gttcacgctg cgcgtcgagc
     1141 tgcccgagca gtgggacgtc gtccgcatcg tcgccccgcc taacgaacgc gtgctcgcgg
     1201 tgaaagtcgc cgccctggcc gtgctcgacc cgcgcgccga cgtccgcgag ttcgtcatca
     1261 agatcaacgg acgcgaagtg ctggacgaac agcgcagcgt gtccgacgtc ggcgcgcgcg
     1321 acgggtcaat cttcctcatc acgcaccgcc gccgccgacc cgtccggtga cccgccgggc
     1381 ctcctgccta acgacgccgc ccggcgcccg cgggccgtga gcgtcgcctt catttctcac
     1441 gccgactgcg gccgccacga caccggctgg ggccaccccg aacacgtcgg ccgcctccgc
     1501 gccatcaccc gcgcgctccg cgaacggccc gacctcttcg gaaccatcga gcacgtcgaa
     1561 ggccgccacg ccaccgcaca ggagctcgcg ctcgcgcacg accccatcta catccgcgaa
     1621 gtcgaagaga tcgcgagcgc cggcggcgga gcgctcgatg cggatacggt ggcaagcgaa
     1681 gggtcctggg cggcagccac cgcggccgcg ggctgcgtcc tcgatggcgt cgacatggcc
     1741 ctcgatggac gcgccgtccg cagcttctgc gcggtacgcc cgcccggcca tcacgcgctc
     1801 cgcgatcgcg ctatgggatt ctgcctcttc ggcaatgtcg cagtcgcggc gcactatgcg
     1861 cgtcgcacgc acgggctcga ccgcgtgctc atcgtcgact gggacgtgca ccacggcaac
     1921 ggcacccaag cgctcgtcga ggccgatcac ggcatccatt tcgtgtccat gcaccagtgg
     1981 ccctggtatc ccggcagcgg cgccgccgac gatcacggca acggcaacgt gtggaacgtc
     2041 ccactgccgg ctggactccc agcgcagcag tacgtcgacg ccctcgagca aggcatcgat
     2101 cgcgccaccg acggtttcac gccggatctg gtgctcgtct ccgcgggctt cgactcgctc
     2161 cgcggcgacc cccttggcgg cttcacgctg gagctcgagg acatcacgag gctcacgcgc
     2221 cacctcacgg atcgcgccgc acagtggtgt ggcgggcgag tcgtcggcgc gctcgaaggg
     2281 ggctatgcgc ccgaccgcgt gggcgaggcg tgcgtcacga tgctcgccga gttggcgcag
     2341 acccgctgag gtgcagcggg tagagagcag ccgagcgatc cgtgctttgt ccgtgctgtc
     2401 atccgttcaa tccgtatcat caaagcgagg agacccatta cctcgcgtct tgctccggca
     2461 ctggccccgc gtcgggcgcc atgtctgaac cggtaccgca ctcccaccgc attcacgttg
     2521 cgcataactt ccgatgctat ggcgttgact cagtcggcac atccggtcgg agcgtgacgc
     2581 cgtggctcgc cacgtagccg caagcggcgt gcgattgttc ggcgtcatgt cggttgcgcg
     2641 cgatagcgat ggcgggcatc cgggggaggc aagcctcgtt cgcacgcgcg atctggcggc
     2701 gctcgtcaag caagtccccg attctcgtcg ggatctggac gacgccgaca tcgacctcca
     2761 tcaacgcgtc gtcgagtcgg cgttcgagcg cggcggcgtg ctgccggcgc cctgcggcac
     2821 cgtgtttcgt aatgcggagc aactgcgacg ttggatggaa cagaactata tcgcgctctc
     2881 tgaaggctta cagttcgtcg ccggtcgctc ggaggcgcgc gtccatatcg cgcgtcgccc
     2941 aactacggaa gccgaagcct cgacgcccgc gacgtcggct gtgctaaccg actgcttcag
     3001 gacgctgagg cgtgtctcgt ccgccgccat gacgctgccg ccggtgcatc atcccgagca
     3061 cgcgttgttc agcgccgcgt ttctcctgga ccgcgaacgc tgggcagagt tcgagaccac
     3121 ggtgcggtcg ctggcgcatc gcca
//
LOCUS       DASUCJ010000087         1367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_138601, whole genome shotgun sequence.
ACCESSION   DASUCJ010000087 DASUCJ010000000
VERSION     DASUCJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138601"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..604
                     /locus_tag="VFU90_01010"
     CDS             14..604
                     /locus_tag="VFU90_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478373.1"
                     /translation="MIIAPDATEEAIALVAAKKNLRLLLAGGLADPRAAGVTAKTVAG
                     GLLVQTRDNAVVDDMDLRPVTRRAPTNAELRDLRFAFRVAKHVKSNTIVYAKDLATVG
                     IGAGQMSRVDAARIAARKAQDAAREAGLREPATKGSVVASDAFFPFADGLLVAIEAGA
                     TAVIQPGGSMRDDEVIAAADAHGIAMVFTGTRHFRH"
     gene            complement(633..959)
                     /gene="trxA"
                     /locus_tag="VFU90_01015"
     CDS             complement(633..959)
                     /gene="trxA"
                     /locus_tag="VFU90_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5478374.1"
                     /translation="MADGILTLSDATFDEQIGSAETPVVVDFWAEWCGPCRMIAPALE
                     EISNSLNGKVKIVKLNVDENPGTAAKYGIMSIPTLMLFKDGQLASRQIGAQPKQKLEQ
                     WISGSI"
     gene            complement(975..>1367)
                     /locus_tag="VFU90_01020"
     CDS             complement(975..>1367)
                     /locus_tag="VFU90_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5478375.1"
                     /translation="MQDRAFGNAKIDFAWNSVVEDVIGDGRVEALRLKDTETGATSDL
                     AVTGVFVAIGHEPNTKLFVGQLDLDPGGYILTLPGTTHTSVRGVFAAGDVQDHVYRQA
                     ITAAASGCMAALEAERYLEALGDATRDA"
BASE COUNT          239 a          448 c          419 g          261 t
ORIGIN      
        1 aattttcacc gaagtgatca tcgcgcccga tgccaccgag gaggcgatcg cgctggtcgc
       61 agcgaagaag aacttaaggc ttctgcttgc cggcgggctg gccgatccgc gcgccgccgg
      121 cgtgaccgcg aaaaccgtcg ccggcggact tctggtgcaa acccgcgaca acgcagtggt
      181 cgacgatatg gatttgcggc cggtgacccg gcgcgcgcca accaatgccg aattgcgcga
      241 tttacgtttt gcgttccggg tcgccaagca cgtgaagtcg aacacgatcg tttatgccaa
      301 agatttggcg acggtcggca tcggtgccgg ccagatgagc cgcgtcgatg ccgcgcgcat
      361 cgcggcacgc aaggcgcagg acgccgcgcg cgaagccgga ctgagagagc ccgccaccaa
      421 agggtcggtg gtcgcttccg acgctttttt cccgtttgcc gacgggttgc tcgtcgccat
      481 cgaagccggc gcgaccgcgg tgatccagcc tggcgggtcg atgcgcgacg atgaagtgat
      541 cgcggctgcc gacgcccacg gcatcgccat ggtcttcacc ggcacgcggc atttccggca
      601 ctagcgtgga agacgcgtcg ccgcgcgtta ggtcagatcg agccgctaat ccactgctca
      661 agcttctgct tcggctgggc gccgatctgg cgcgatgcca gctggccgtc cttgaacagc
      721 atcaaggtcg gaatcgacat aatcccgtat ttggccgccg tgccggggtt ttcatcaaca
      781 ttgagcttga cgatcttcac cttgccattg agcgagttgg aaatctcttc aagcgcaggt
      841 gcaatcatcc ggcacggacc gcaccattcc gcccagaaat caacgacgac cggcgtctcg
      901 gcactgccga tctgctcgtc gaacgttgcg tccgacagtg tcagaattcc gtcagccaca
      961 gctgttcctt tcgttcaagc gtcgcgggtg gcgtcgccga gcgcttccag atagcgctcc
     1021 gcttcgagcg ccgccatgca cccgcttgcc gcagcggtga tcgcctgcct gtatacgtgg
     1081 tcttgcacgt cgccggctgc gaacactcct cgcaccgacg tgtgcgtcgt gccgggcaac
     1141 gtcaggatgt agccgcccgg gtcgaggtcg agctggccga cgaacaactt cgtgttcggt
     1201 tcatgaccga tcgcgacgaa tacgcccgtc actgcaaggt ccgaagtcgc accggtctcc
     1261 gtgtccttca accggagcgc ttcgacgcga ccgtcgccga tcacgtcttc cacgacgctg
     1321 ttccaggcga agtcgatctt cgcgttcccg aacgcgcggt cctgcat
//
LOCUS       DASUCJ010000088         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1040228, whole genome shotgun sequence.
ACCESSION   DASUCJ010000088 DASUCJ010000000
VERSION     DASUCJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040228"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2920
                     /locus_tag="VFU90_01025"
     CDS             <1..>2920
                     /locus_tag="VFU90_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEU5478376.1"
                     /translation="ADAPTGKWQVEFRLDPASKEATQSIAFRIEEFLPERLKLDLTSA
                     APTIAPGAPLKLDVASDYLYGAPAAGNRFTARLTLAPDVHPVGAHADYFLGDPTLDLP
                     KEAKDVVDETLDENGKLTTDIAILDGKKPDAPVAAIVSGSVYETGGRTVTRTLKRTLW
                     PADALVGVRPLFDPKDGSNANARAGFELIRSDAKGNQLAASKLGVKLVRENRAYHWTY
                     DNDSGWHFDYNTNFEDMQSQTVDIPAGGVAKIDAPVEWGNYRLDVTDPATGLTTRFPF
                     FAGWSWNDDNRGKEARPDKVKLALDKPSYRAGDTLKVTVTPPHAGPGVLLVESDHLLY
                     TRDIDARDGATLEIPVTKDWERHDVYLTALVFRGGSAADKITPARAVGEAFVPMDRSA
                     RKIDVAVDAPKTMKPEIDLPVKVMAPALAGKKAFVTVSAVDVGILNITRFARPDPLGW
                     FFAQRGLGVDAYDLYGRVIESFDGATAKLRYGGDMALAALPQARRPTAHVQTVDLFSG
                     VVALDAQGNATVALKMPDFNGTVRVTALVFGENSYGANDTETIVRAPLVAEISTPRVL
                     APGDKAEVTLDLQNFSGAAQEFAVKVSADKLLAIAQGERKVKLADQAKTTLNFPLTAQ
                     QGYGVGKIDVSATSLSTSGGGIKIDRHFDLPVRAAWPAVLRSSARSLDKLVPITLGAD
                     ALDGLLPDSVTARLTVSSLPPLPFASALAGLLQYPYGCIEQTTSKAWGNLLLDEKTAD
                     NLHVAGIAPDQRKQRIDAAIGRIASMQIPSGNFSMWGGDSYVPTFLTPYVVDFLESAR
                     EEGFVVADDVLQKALKRLNDDLLAGGHPYYDYEHSDAMRFADEAYSGYVLARVNRAPL
                     GTLRALFDNDRSKSITALPLVHLGIALKLMGDQPRAEKAIAEAFAKKVDRPWYLGDYG
                     SDLRDTALMVALSHKYGLAKPEYDARVFDLARSLTSQQRDADAEQKRYGFHWNWLSTQ
                     "
BASE COUNT          539 a          962 c          954 g          465 t
ORIGIN      
        1 aggccgatgc gccgaccggc aagtggcagg tcgaattccg cctcgacccg gcgagcaagg
       61 aagcgaccca aagcattgcg ttccgcatcg aggaattcct gcccgaacgc ctcaagcttg
      121 acctgacgag cgccgcgccg acgatcgcgc cgggggcgcc gttgaaactc gacgtcgcct
      181 ccgattacct ctacggcgcg ccggcggcgg gcaaccgctt caccgcgcgc ctgacgctcg
      241 cgccggacgt gcatccggtg ggcgcgcacg ccgactattt cttgggcgat ccgacactgg
      301 atctgccgaa ggaagccaag gacgttgtgg atgaaacgct cgacgaaaac ggcaagctga
      361 cgaccgacat cgcgatcctc gacggcaaga aacccgacgc gccggtcgcg gccatcgtgt
      421 ctggcagcgt ctatgagacc ggcggacgca ccgtcacgcg cacgctcaag cggacgctgt
      481 ggccggccga cgcgctggtc ggcgtgcgtc cgctgttcga tcccaaggac ggctcgaacg
      541 cgaatgcgcg cgccggcttc gaattgatcc gcagcgacgc gaagggcaac cagctcgcgg
      601 cgagcaagct cggcgtgaaa ctggtgcgcg aaaaccgcgc ctatcactgg acctacgaca
      661 acgacagcgg ctggcatttc gactacaaca ccaatttcga ggacatgcag tcgcagacgg
      721 tcgatattcc tgccggcggc gtggccaaga tcgatgcgcc ggtggagtgg ggcaactatc
      781 gtctcgacgt gaccgatccc gcgactggcc tgacgacgcg cttcccgttt ttcgccggct
      841 ggagctggaa cgacgacaac cgcggcaagg aagcgcgccc ggacaaggtc aagctcgcgc
      901 tcgacaaacc aagttatcgc gccggcgaca cgctgaaggt gacggtcacg ccgccgcatg
      961 ccggaccggg cgtgctgttg gtcgaatccg atcatctgct ctacacgcgc gacatcgacg
     1021 ccagggacgg cgcgacgctc gagattccgg tcacgaagga ctgggaacgc cacgacgtgt
     1081 acctgactgc gctcgtcttc cgtggcggct cggcggcgga caagatcacg ccggcgcgcg
     1141 ccgtgggtga ggccttcgtg cccatggacc gcagcgcacg caagatcgat gtggccgtcg
     1201 acgcaccgaa gaccatgaaa cccgagatcg acctgccggt gaaggtgatg gcgccggcgc
     1261 tggccggcaa gaaggcgttc gtcaccgtgt ccgcggtcga cgtcggcatc ctcaacatca
     1321 cgcgcttcgc gcggcccgat ccgctgggct ggttcttcgc gcagcgtggt ctcggcgtgg
     1381 acgcgtacga tctgtacggt cgcgtcatcg agagtttcga cggcgcgacg gcgaagttgc
     1441 gctatggcgg cgacatggcg ctggcggcgc tgccgcaggc gcggcgcccg accgcgcacg
     1501 tgcagactgt tgacctgttc agcggcgtgg tcgcgctgga tgcgcagggc aacgcgaccg
     1561 tcgcgctgaa gatgccggac ttcaacggca ccgtgcgcgt caccgcgctg gtgttcggcg
     1621 agaacagtta cggtgcgaac gacacggaaa ccatcgtgcg tgcgccgcta gtcgcggaga
     1681 tctccacgcc gcgcgtgctc gcgcccggcg acaaggcgga agtcacgctc gacctgcaga
     1741 atttctccgg cgccgcgcag gaattcgcgg tgaaggtgag cgccgacaaa ttgctggcga
     1801 tcgcgcaggg cgagcgcaag gtcaagctcg ccgaccaggc caagaccacg ctcaatttcc
     1861 cgctgaccgc ccagcaaggt tatggcgtcg gcaagatcga cgtgagtgcg acatcccttt
     1921 caacatcggg cggcggcatc aagatcgatc ggcacttcga tttgcccgtg cgtgcggcct
     1981 ggccggccgt gttgcgcagc agtgcacgat cgctcgacaa gctcgtgccg atcacgctgg
     2041 gcgccgacgc actggacggc ctgctgccgg attcggtgac cgcgcgcctg accgtatcga
     2101 gtcttccgcc tctaccgttc gcgagcgcgc tcgccggatt gctgcaatat ccctacggct
     2161 gcatcgaaca gaccacgagc aaggcctggg gcaatctgtt gctcgacgag aaaaccgcgg
     2221 acaacctgca tgtcgccggc atcgctccgg atcagcgcaa gcagcgcatc gacgcggcga
     2281 tcggacgcat cgcctcgatg cagattccgt cgggcaattt ctcgatgtgg ggcggcgaca
     2341 gctatgtgcc gacgttcctg acgccctacg tcgtcgattt cctcgaaagc gcgcgcgagg
     2401 aaggattcgt cgtcgccgac gatgtgctgc aaaaggcgct caagcgcctg aacgacgatc
     2461 tcctcgccgg tggccatccc tactacgact acgagcacag cgacgccatg cgctttgccg
     2521 acgaggcgta ttccggctac gtgctcgcgc gcgtgaaccg cgcgccgctc ggcaccttgc
     2581 gtgcgctgtt cgacaacgat cgttccaaat cgatcaccgc attgccgctg gttcacctgg
     2641 gcatcgcgct gaagttgatg ggtgatcagc cgcgcgccga gaaagccatc gccgaggcgt
     2701 ttgccaagaa agtagacagg ccatggtatc tcggcgacta cggctcggat ctgcgcgaca
     2761 ccgcgctgat ggtggcgctg tcgcacaagt acggcttggc caaacccgag tacgacgcgc
     2821 gcgtgttcga cctcgcgcgt tcgctgacct cgcagcagcg cgatgccgac gccgagcaga
     2881 agcgctacgg cttccactgg aactggctga gcacgcagga
//
LOCUS       DASUCJ010000089         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_505301, whole genome shotgun sequence.
ACCESSION   DASUCJ010000089 DASUCJ010000000
VERSION     DASUCJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFU90_01030"
     CDS             <1..864
                     /locus_tag="VFU90_01030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5478377.1"
                     /translation="HLGATPVFVDVRPDTWCIDADLVERAITPRTKAIVATHLYGNLC
                     DMERLLRIGETHGVPVIEDAAEALGSVYRDRRAGSLGRFGVFSFHGSKTVTTGEGGML
                     VTNDAGLYERVLTLSNHGRSRTGTRQFWPEVVGYKYKMSNIQAAIGCAQMERIDDLLA
                     RKRAILRSYRERIERIRGAAMNPELTGTINGAWMPTVVFDEAAGVTGEALAAAFRSAN
                     IDARVFFSPLSSLPMFGGEATSPIAASLPGRAINLPSYHDMNAADIDRVADVVIQVAD
                     AARAKRREPAA"
     gene            881..>1149
                     /locus_tag="VFU90_01035"
     CDS             881..>1149
                     /locus_tag="VFU90_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEU5478378.1"
                     /translation="MTAEDDRQAFEDNKRRKAERLGRDSAVLGQVRKTLRALDEYDYS
                     YLWTWMGVPIIQLPADVMATQEIVWATRPDVIIETGVARGGSVVF"
BASE COUNT          208 a          382 c          372 g          187 t
ORIGIN      
        1 cacctcggcg cgacgcccgt cttcgtcgat gtccggcccg acacctggtg catcgatgca
       61 gaccttgtcg agcgcgccat cactccgcgc accaaagcga tcgtcgcaac gcatctctac
      121 ggcaatctct gcgacatgga gcggcttcta cgcatcggtg agacgcatgg cgtgcccgtt
      181 atcgaggacg cggcggaggc tttgggttcg gtctaccgcg accggcgcgc cggttcgctg
      241 ggccgcttcg gcgtcttctc gttccatgga tccaagacgg tgacgaccgg cgaaggtggc
      301 atgctcgtca caaacgatgc cggcctctac gaacgcgtcc tgaccttgag taatcacggc
      361 cgctcgcgga ccgggacgcg gcaattctgg ccagaggtcg tcggctacaa atacaagatg
      421 tcgaacatcc aggcggcaat tggctgtgct cagatggagc ggatcgacga tcttcttgcg
      481 cgcaagcgcg ccatcctgcg cagttaccgc gaacgcatcg aacgcatccg cggcgcggcg
      541 atgaacccgg agctgaccgg aacgataaat ggcgcgtgga tgccgacggt cgtattcgac
      601 gaggcggcgg gcgtgacggg cgaggcactg gccgcggcgt tccggagtgc aaatatcgac
      661 gcgagagtct tcttctcgcc gctctcctcg ctgccgatgt tcggcggcga ggcgacgagc
      721 ccgatcgccg cctcgttgcc cggacgagcg atcaacctcc cctcctacca cgacatgaac
      781 gccgcggaca ttgaccgcgt cgccgatgtc gtcatccagg tcgccgacgc ggcccgcgcc
      841 aagcggcgcg agccggcagc ttgacgaaag acgcagcacg atgaccgccg aagacgaccg
      901 ccaggccttt gaggacaaca agcgccggaa ggctgagcgt ctcggccgcg acagcgcggt
      961 actcggccag gtgcgcaaga cgctccgcgc cctcgacgag tacgactaca gctatctctg
     1021 gacctggatg ggcgtgccga tcatccagct ccccgccgac gtcatggcga cgcaggagat
     1081 cgtctgggcg acgcggccgg acgtgatcat cgaaacaggt gtcgcgcgcg gcggctcggt
     1141 cgtcttcat
//
LOCUS       DASUCJ010000090         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_92689, whole genome shotgun sequence.
ACCESSION   DASUCJ010000090 DASUCJ010000000
VERSION     DASUCJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92689"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFU90_01040"
     CDS             complement(<1..999)
                     /locus_tag="VFU90_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478379.1"
                     /translation="MADRDGATIEAWLLDAHTSAAAAAIGALTACENRASELARFGAA
                     VYSLGGCDRALAALLDPQLLACRSFADVETYLATLDSPDDRELLPAGVVLAWAGDAAG
                     AHRLLRAAHERALAGRRIAFAVGALERCARHALLFGDVAAARIAIEDAASLAARNRLE
                     RWRLRCAAAAAALALDAGDVEPARRLVDDAQSAPHSSEMLALFAPTGAALGIFEENAA
                     ALAEWTGESIVDVALHSRRTEAAIAATVACLLAAGTQPPLRTAVANALRRALMQAENA
                     AAAPDLFSLAARYGEIGDARLAVAALGAVCSPERRYLAAQRLLATAHWEFRFGERSR"
     gene            1100..3718
                     /locus_tag="VFU90_01045"
     CDS             1100..3718
                     /locus_tag="VFU90_01045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5478380.1"
                     /translation="MNLPKQYDPKTVEPRLYRQWEQGGYFHEEPDPARPPFVVVMPPP
                     NVTGRAHLGHGSTYTPMDVLVRYHRMLGDNADWLPGLDHAAIATEAVLVRELAKEGLS
                     RESLGRDAFVRRAWDWSKQYGGEIERQFRVLGFGPDWQRRRFTMDEPPSAAVRKVFVQ
                     LYREGLIYRGKRLVNWDPKGKTTVSDAEVDHVERDATLWHVTYPFEGGARGERRGITV
                     ATTRPETILADVAVAVHPDDERYADLIGTRVLVPPLRARSIPIVADAAVDPAFGTGAV
                     KVTPAHDAVDYDIGLRHQLPMPSVIGLDARIAGDDIPVGPYEGLDRFDARARIVEDLR
                     AEGLLVKEEPHRHAIAVSERSGDVIEPLLSLQWFLKMESLAKPALDAYREGRLKFVPE
                     RYGRTYEQWLENIRDWNVSRQLWWGHRLPVWYTPDGTPVVAESEAQARELALREHNTA
                     ELTRDPDTLDTWFSSGIWPFSMLGWPEKTLELECWYPSQVMVTGWEIIFLWVARMVML
                     GIHILGRLPFPEVFITPLVFDAQGRKMSKSLGNAIDPIELAERYGADAFRMGILRQMR
                     IEGQEIRFQESRCEEARNFNNKIWNATRYLLALPEGLPDALTLPPAASLTAADRWILT
                     RLHDAIRQVAGALDGYDVGAAAESIWRFIWYEFCDWYLEATKDPVNARTRAAVLSFVW
                     NNAMRLLHPICPFISEEVWLALPHDGRSITTATWPDPLEVPVDREAAADFEAIARAAE
                     RVRNARADMGLAPKDRAALEIPENVPEAAAALLARFTAGDVERVPAAGTTPRDALALV
                     TLRAPHGVLAARYRRDAERLRAEVERLERKLANEQFVAKAAPGVVAKEREKLEGYRQE
                     YVRVEAALAAIGEPVR"
     gene            3715..4254
                     /gene="pyrR"
                     /locus_tag="VFU90_01050"
     CDS             3715..4254
                     /gene="pyrR"
                     /locus_tag="VFU90_01050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5478381.1"
                     /translation="MTTTPKRVLLRSDEIARIVARVAHQILEPDDAQRGLVLLGVRRG
                     GEALAARLAAEIERISGRAPMLGFLNINLYRDDRVTHQMPESQIPADVSDRTVVIVDD
                     VLFTGRTIRAALDAVTDLGRPAAIRLCVLIDRGLRELPIQPDYVGRIIPTSRRERVDV
                     SLAPEPAATDEVAILADTA"
BASE COUNT          729 a         1437 c         1545 g          694 t
ORIGIN      
        1 tcgcgatcgt tcgccgaagc gaaactccca atgcgcggtc gcgagcagcc gctgagcggc
       61 cagatagcgg cgctcggggg agcagacggc gccgagcgcc gcgaccgcca aacgtgcgtc
      121 gccgatctca ccgtagcgcg cggcgagcga aaacaggtcg ggtgccgcgg ctgcattttc
      181 ggcttgcatc agcgcgcgcc gcaacgcgtt ggcgaccgca gtccgcagcg gcggctgggt
      241 gcctgcagcg agcaggcatg cgacggtcgc cgcgatcgcc gcctcggtac ggcgggaatg
      301 cagcgcgacg tcgacgatac tttccccggt ccactcggcc agcgcggcgg cattttcttc
      361 gaaaatcccg agcgccgcgc cggtcggggc gaagagtgcg agcatttcgg acgagtgcgg
      421 cgcgctctgc gcgtcgtcca cgagacggcg cgccggttcg acgtcacccg catcgagtgc
      481 gagtgcggcg gcagccgccg cgcagcgcag ccgccagcgt tccaatcggt tccgagcggc
      541 taggctagcc gcgtcctcga tcgcgatccg cgccgcggcg acgtcgccga agagcagcgc
      601 gtggcgagcg catcgttcga gcgcgccgac ggcgaacgcg atccgccggc cggcgagcgc
      661 gcgctcgtga gcggcgcgca gcagacggtg cgcaccggct gcatcgcctg cccaagcgag
      721 caccacgcct gccggcaaga gttcgcgatc gtcgggtgag tcgagcgtcg cgagatacgt
      781 ctcgacgtcc gcgaagctgc ggcacgcgag cagctgcgga tccagcaacg ccgcaagcgc
      841 gcgatcgcat ccgcccagcg agtacaccgc ggcgccgaac cgtgcgagtt ccgatgcgcg
      901 gttctcgcac gccgtcagtg cgccgatcgc ggccgcggcc gccgacgtat gggcgtcgag
      961 aagccatgcc tcgatcgtgg cgccgtcgcg gtctgccatt ggggccgatg ctagcaagcg
     1021 tcggttaagg cgcgattacg aacggctaaa gaatgcttcg aggttccggt gcttgccgtg
     1081 cggaacgcag ggcggactca tgaacctccc caagcagtac gacccgaaaa ccgtagagcc
     1141 acggctgtac cggcaatggg agcagggcgg ctattttcac gaagagcccg acccggcccg
     1201 ccccccgttc gtcgtcgtca tgcccccgcc gaacgttacc ggacgcgcgc accttggcca
     1261 cggctcgacg tacacgccga tggacgttct ggtgcgctat caccggatgc tcggcgacaa
     1321 cgccgattgg ctccccggtc tcgaccacgc cgcgattgcg accgaggcgg tgctggtgcg
     1381 cgaactcgcc aaggagggtc tgagccgcga aagcctcggc cgcgatgcat tcgtccggcg
     1441 agcctgggac tggtcgaagc agtacggtgg tgagatcgag cggcagttcc gcgtcctcgg
     1501 cttcggtccg gactggcagc gccgccgctt cacgatggac gagccgccgt cggcggcggt
     1561 gcgcaaggtg ttcgtccagc tctatcgcga ggggctcatc tatcgcggca aacggctcgt
     1621 caactgggat ccgaaaggaa agacgaccgt ctcggacgcc gaggtcgatc acgtcgagcg
     1681 cgacgcgacc ctatggcacg taacctatcc gttcgagggc ggcgcgcgcg gcgagcggcg
     1741 cgggattacg gttgcgacga cccggccgga aaccatcttg gccgacgtcg cggtagccgt
     1801 acatccggac gacgagcgat acgcggacct gatcggaacg cgagtgctgg tgccgccgct
     1861 gcgcgcacgc tcgattccga tcgtcgccga tgcggcggtc gatccggctt tcggcaccgg
     1921 ggcggtgaag gtcacgccgg cccacgacgc ggtcgattac gacatcgggc tgcgccacca
     1981 gctgccgatg ccgtcggtca tcgggttgga cgcgcggatc gcgggcgacg acatcccggt
     2041 ggggccctac gaggggctcg accggttcga cgcgcgcgcc cggatcgttg aagacctgcg
     2101 cgccgaaggg ctcctggtaa aggaagagcc gcatcgccat gcgatcgcgg tgagcgagcg
     2161 ttcgggcgac gtgatcgagc cgctgctctc gctgcagtgg ttcctgaaga tggagtcgct
     2221 ggcgaagccg gcgctcgacg cgtatcgcga aggacgcttg aagttcgtcc ccgagcgcta
     2281 cggccgcacg tacgaacagt ggctcgaaaa catccgggat tggaacgtct cgcgccagct
     2341 ctggtggggg catcggctgc cggtctggta cacgcccgac ggaacgccgg tcgtcgccga
     2401 gtcggaagcg caagcgcgcg agctcgcgct gcgcgaacac aacacggcgg agctgacgcg
     2461 cgatccggat acgctcgaca cctggttttc gagcgggatt tggccgttct cgatgctcgg
     2521 ctggccggag aagacgctcg agctggagtg ctggtaccct tcgcaagtga tggtcaccgg
     2581 ttgggagatc atctttctgt gggtggcccg catggtaatg cttgggattc acatcctcgg
     2641 gcggctgccg tttcccgagg tattcatcac gccgttagtg ttcgacgcgc aaggtcgcaa
     2701 gatgtcgaag tcgctcggca acgcgatcga cccgatcgag ctggcggagc gctacggggc
     2761 cgatgccttc cggatgggga tcctgcggca aatgcggatc gaggggcaag agattcgctt
     2821 tcaggagtcg cgttgtgagg aagcgcgcaa cttcaacaat aagatctgga acgcgacgcg
     2881 ttacctgctg gcgcttcccg aggggcttcc ggatgcgctg acgctcccgc cggcggcgtc
     2941 gctcacggcg gccgaccggt ggattctcac gcggctgcac gatgcgatcc ggcaagttgc
     3001 cggcgctctc gacggctacg acgtgggtgc ggcggccgag tcgatctggc ggttcatttg
     3061 gtacgagttc tgcgactggt acttggaggc gaccaaagat ccggttaacg cacggacgcg
     3121 cgctgcggtg ctttcgttcg tatggaacaa cgcgatgcgt ttgctgcatc cgatctgtcc
     3181 gttcatcagc gaagaagtct ggctggcgct gccgcacgac ggccgcagca tcacgaccgc
     3241 aacctggccc gatccgcttg aagttccggt cgaccgcgag gcggcggccg atttcgaggc
     3301 aatcgcgcga gcggccgagc gggtgcgcaa cgcacgcgcc gatatggggc tcgcacccaa
     3361 ggaccgggcc gcgctcgaga tccccgagaa cgttcccgag gcggcggccg cactgctggc
     3421 acgcttcacc gcgggcgacg tcgagcgcgt gccggccgcc ggaacgactc cgcgcgacgc
     3481 gctcgccctg gttacgctgc gggctccgca cggcgtgctg gcggcccgct atcggcgtga
     3541 cgccgaacgc ctgcgcgccg aggtcgagcg gcttgagcgc aagcttgcca acgagcagtt
     3601 cgtggccaag gcggctcccg gcgtggttgc gaaggagcga gagaagctcg aaggataccg
     3661 ccaggaatat gtccgcgtcg aagcggcttt ggccgcgata ggagaaccgg tgcgatgacg
     3721 accacgccga agcgcgtgtt gctgcgcagc gacgaaatcg cccgtatcgt ggcccgcgtc
     3781 gcgcatcaga ttctcgaacc ggacgacgcc cagcgcgggc tggtactgct cggcgttcgg
     3841 cgcggcggcg aagcgctcgc ggcgcggctg gcggcggaga ttgagcgcat cagcgggcgc
     3901 gcaccgatgc tcggctttct caatatcaac ctctatcggg acgatcgggt tacgcaccaa
     3961 atgcccgagt cgcagattcc ggccgacgtc tcggaccgaa cggtagtcat agtggacgac
     4021 gttctgttca ccggccgcac gattcgcgcc gcactcgacg cggtaacgga tctcgggcgc
     4081 ccggccgcga ttcggctgtg cgtactgatc gaccgagggc tgcgcgagct gccgattcaa
     4141 cccgattacg tcgggcggat tattccgacc agccgccgcg aacgcgtcga cgtgtcgctg
     4201 gcgcccgaac cggccgcgac cgacgaggtg gccattcttg cggatacggc gtagcctgat
     4261 cgatctctcg gacctcgacg ccgaagagat tgcctacgtc ttcgagcgca cggctgcgtt
     4321 cgagcaccag ccgcccggca aactgctggc ggggatcgcg tgcgtgaaca tgttcttcga
     4381 gcagagcacg cgcacgttta cgtcg
//
LOCUS       DASUCJ010000091         7406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_948473, whole genome shotgun sequence.
ACCESSION   DASUCJ010000091 DASUCJ010000000
VERSION     DASUCJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948473"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFU90_01055"
     CDS             <1..1113
                     /locus_tag="VFU90_01055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5478382.1"
                     /translation="LLHKVEAEMHERKAAFRGLYTCSDGNDGSDYVSRLERPFVTSTS
                     TNFGAEGCDLAGGTSYLFARDELEGKFDYLFIDEAGQVSLADALAVSTCARNVVLLGD
                     PAQLAQVGQGIHPLHVADSILQHLLGDAATVPPNRGVFLDESYRMQPEICKYVSAMSY
                     EGRLEAEAQTRLHGVTADGVSRAGLEYLPVEHAGNASSSIEEADAIVREVALLLRGQV
                     TDSRPPELCGKPRPMRERDIMIVTPYNAQRRLITSKLKAAGISVEPGAGVEVGTVDKF
                     QGREAAVVFYSMATSSGEDAPRDMTFLFEQNRFNVAISRARALSVLVCSPRLLETACR
                     TPEQMALVNLLCEFAERASRPRAGEPHGDRDRAAIA"
     gene            1110..1943
                     /locus_tag="VFU90_01060"
     CDS             1110..1943
                     /locus_tag="VFU90_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5478383.1"
                     /translation="MSIGLREKGERFRALHARPGAFVIPNPWDAGTARILAALGFEAL
                     TTTSAGLAFALGRRDGAGRITRDETLANAKAVADAIDLPVAADLENGFAETPAGVAET
                     IRLAGESAGLAGGSIEDATGDPARPIYELAEAVERITAAAEAARALLFPFVLVGRAEN
                     FLHGRPDLGDTIRRLQAYEAAGADALYAPGVTKPDDIRAICAAVGKPVNVVAGLRDDA
                     FSVEQLAALGVRRISVGSTLARAALGAFVRAATEIRDRGTFTFAADAIPYARAQEYME
                     P"
     gene            complement(2103..3659)
                     /gene="aceF"
                     /locus_tag="VFU90_01065"
     CDS             complement(2103..3659)
                     /gene="aceF"
                     /locus_tag="VFU90_01065"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEU5478384.1"
                     /translation="MNSAIEVRVPDIGDFKDVPVIEVLVKPGQAIAKETPLIVLESEK
                     ASMEVPSTAAGTVDGVNVRVGDKVSEGCVILTLRDATAAPSGGAPAPASAAAPAPATI
                     ELKVPDIGDFKNVPVVEVLVRPGQTVAAEAPLLVLESEKASMEVPAAAAGTIADVRLK
                     AGDKVSQGDVIATLTVAGQPPAAAPPPAAPPTAAPPPAPAETAPAAAAALPPAPSTGD
                     GIVHASPAIRRFARELGVELRGLRGSGPHGRVTREDVQGFVKNALQRTPGGGPSIGGG
                     LPAWPQIDFSQFGEIERRPLSRIKKLSGPNLHRNWVQIPHITNYDQADVTELETFRTE
                     INAEQAKRGGPKLTMLAFLIKASVAALQRFPEFNASLDGDELVYKRYYNVGFAADTPG
                     GLVVPVVRNADSKGLLALAAETAELAAKARDGKLPLRDMQGGSFTISSIGGIGGTAFT
                     QIVNAPEVAILGAVRAAIQPVWNGTSFVPRTIMPISLSYDHRVIDGAGAARFLVYLAS
                     LLSDFKRVAL"
     gene            complement(3656..6319)
                     /gene="aceE"
                     /locus_tag="VFU90_01070"
     CDS             complement(3656..6319)
                     /gene="aceE"
                     /locus_tag="VFU90_01070"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5478385.1"
                     /translation="MTASVHDPDPAETQEWVDAMRGVVTLEGPDRARELIGRVVDEAQ
                     RGGAHVSLGLETPYVNTIPVEEQPPMPGDRELEAHLRHYIRWNAIATVLRANKVSSEL
                     GGHLASFASAATLYDVGFNHFFRAPSETFGGDLVFLQGHSAPGFYARAFLEGRITESQ
                     LDHFRQEVDGQGLSSYPHPWLMPEFWQFPTVSMGLGPIMSIYQARFMRYLHDRALIDA
                     RDRKVWAFHGDGEMDEPESLGAISLAGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQE
                     LERVFKGAGWNVIKVIWGSSWDRLLREDGSGRLVQLMNECVDGDYQTFKSRDGKYVRE
                     AFFGRYPETAALVADWTDDQIWALQRGGHDSPKVYAAYRRAVEHRGQPTVILAKTIKG
                     YGMGAAGEARNIAHQAKKMEVEAMRAFRDRFAIPLSDAEIEKIPFYKPAEDSPEMRYL
                     REREQTLGRVPQRRRKSASLAVPELSAFEQQLQGTGDREISTTMAFVRILNTIVRDAA
                     VGPRVVPIVADESRTFGMEGMFRQLGIYSSVGQLYHPQDAEQLMWYREDKLGQILQEG
                     INEAGALSSWIAAGTSYSNHNLPMIPFYIFYSMFGFQRVGDLTWAAGDSRTRGFLLGA
                     TSGRTTLNGEGLQHEDGSSQVAASFVPNCRSYDPTYNYEVATIIQDGLRRMLADQEDV
                     YYYITLLNENYAHPPMPENAREGILRGMYLLRAGAAAEKKAPSVQLFGSGAILREVLA
                     AADLLENEWGVRSDVWSVTSFNELQRDGIATRRWNLLHPEAPPRRSFVEASLDGHPGP
                     GIAATDYIHTFAEQIHPFVGRRYVTLGTDGFGRSDYRRKLREFFEVNRHFVTLAALKA
                     LADEGTVAASMVSKAIKKYRIDPEKPNPITT"
     gene            complement(6410..>7406)
                     /locus_tag="VFU90_01075"
     CDS             complement(6410..>7406)
                     /locus_tag="VFU90_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5478386.1"
                     /translation="RSRAPVCTLLSNTAAQFPRERTHAAETTGTRTPTKWQPQAGPYL
                     LLGGAQLAVGAAAIFARFALTGAGPIAVAASRLCIAALVLLAIASLRRDASARPTRRQ
                     AVLLGAAGIALAAHFGWWIASLDDTSVAVSTLLVATTPVWTAAYDAVAHRRAPSPRTL
                     AAFVIGAIGLTCVVGFGAYENPAHAKPLVGALLALGGALAMAAYLLIVREARGALPTR
                     AIVTRTYAWAAAVLILAAALAHQPPPPLSAAGAWGGILGMALVSQLLGHTAINAALRW
                     FSPSAVAFSTLMEPVSAAILALVVFGEPLPPAVVAGGAVLLAALGLVLSDERLRA"
BASE COUNT         1186 a         2495 c         2477 g         1248 t
ORIGIN      
        1 ttgctgcaca aagtcgaagc cgaaatgcac gaacggaagg ccgcgtttcg cggactctac
       61 acatgcagcg acggcaacga cgggtcggac tacgtatccc ggctcgagcg cccgttcgtc
      121 acctcgacgt cgacgaattt cggcgccgag ggctgcgatt tggcgggcgg cacctcgtat
      181 ctgttcgcgc gcgacgagct cgagggcaaa ttcgattacc tgttcatcga tgaagcgggg
      241 caggtttcgc tcgccgacgc cctggccgtc tccacgtgcg cgcgcaacgt cgttttgctc
      301 ggcgatcccg cgcagctcgc gcaggtcggg caaggcattc acccgctgca cgtcgccgac
      361 tcgatcttgc agcacctgct cggcgacgcg gcgaccgtcc ctcccaaccg cggcgtcttc
      421 ctcgacgagt cgtaccgcat gcagcccgag atctgcaaat acgtctcggc gatgagctac
      481 gaaggacgct tggaagcgga ggcccagacc cgtctgcacg gcgtcaccgc ggatggcgtg
      541 agccgcgccg ggctcgagta tctgccggtc gagcacgccg gcaacgcgtc gagttcgatc
      601 gaggaagccg acgcgatcgt tcgcgaggtc gcgctgctgc tgcgcggcca agtgaccgat
      661 tcccgtccgc cggagctttg cggcaaaccg cggccgatgc gcgaacgcga catcatgatc
      721 gtcacgccct acaacgcgca gcgccggctc atcacaagca agctcaaggc ggcgggcatc
      781 tcggtcgagc ccggcgcggg cgtcgaagtc ggaacggtcg acaagttcca gggccgcgaa
      841 gccgccgtcg ttttctattc gatggcgacc tcgagcggcg aagacgcgcc gcgcgacatg
      901 acgttcctct tcgagcagaa ccgtttcaac gtcgcgatct cccgcgcgcg cgcgttgagc
      961 gtgctggttt gcagcccgcg cttgctcgaa accgcctgtc gcacgcccga acagatggcg
     1021 ctcgtcaatc tcctgtgcga atttgccgaa cgggcgtccc gcccacgcgc cggcgagccg
     1081 cacggcgatc gagaccgagc cgcaatcgca tgagtatcgg gctgcgcgaa aagggcgaac
     1141 gcttccgagc actccatgcg cggccggggg cgttcgtcat accgaatccg tgggacgcgg
     1201 gaaccgcacg cattctcgcg gcgctcggat tcgaggcgct cacgaccacc agcgccgggc
     1261 tggcgttcgc gctcgggcgc cgcgacggcg ccggccggat tacgcgcgac gaaacgctcg
     1321 ccaacgccaa agcggtcgcc gacgcaatcg atttgccggt cgccgccgac ttagaaaacg
     1381 gtttcgccga aacgccggcg ggcgtcgccg aaacgatccg gctggccggc gaaagcgccg
     1441 gtctcgcggg cggatcgatc gaagatgcga ccggcgatcc ggcgcggccg atctacgagc
     1501 tagcggaagc ggtcgaacgc attacggcgg cggccgaggc ggctcgggcg ctgcttttcc
     1561 cgttcgtgct ggtcggccgg gcggagaact ttcttcacgg gcgccccgat ctcggcgaca
     1621 cgatccgccg cctgcaagcc tacgaagcag ccggtgccga cgcgctctac gccccgggcg
     1681 ttaccaagcc ggacgacatc cgcgcgatct gcgccgcggt cgggaagccc gtcaacgtcg
     1741 tcgcgggttt gcgcgacgat gcgttctcgg tcgagcagct ggccgcgctc ggcgtgcggc
     1801 gcatcagcgt cggctcgacg ctcgcgcgcg ccgcgctggg cgcgttcgtc cgcgccgcca
     1861 cagaaatccg cgaccgcggc acgtttacgt tcgcagccga cgcgatcccg tacgcgcgag
     1921 cgcaggagta catggaaccg taggcaccgg gcgctccttc gtccttcgtc cttcgtcctt
     1981 cgacttcgca ctcagcgctg cgcgcttcgt gctccgctca ggatgacacc gccgcgcccc
     2041 gtccttcgac tgcgcactca gcgctgcgcg cttcgtgctc cgcgcaggat gacacggctc
     2101 gcttagagag ccacgcgctt gaaatcggac agcagcgacg cgaggtagac gaggaaccgg
     2161 gcggcgccgg cgccgtcgat gacgcggtgg tcgtagctca ggctaatcgg cattatggtg
     2221 cgaggtacga acgacgtgcc gttccacacc ggctggatcg cggcgcgcac cgcgccgaga
     2281 atcgcaacct ccggggcgtt gacgatttgg gtaaacgccg tgccgccgat gccgccgatc
     2341 gacgagatcg tgaaactccc gccctgcatg tcgcgcagcg gcaactttcc gtcgcgcgcc
     2401 ttggcggcca gctcggcggt ctcggcggcg agcgccagca atcctttcga atcggcgttg
     2461 cggacgaccg gaacgaccag cccgccgggc gtgtcggcgg cgaatcctac gttgtaatag
     2521 cgcttgtaca cgagctcgtc accgtcgagc gaggcgttga actcgggaaa gcgctgcaaa
     2581 gccgccaccg acgccttgat gagaaacgcg agcatcgtga gtttggggcc gccgcgtttc
     2641 gcctgctcgg cgttgatttc ggtgcgaaac gtctccagct cggtgacgtc ggcctggtcg
     2701 tagttggtga tgtgcgggat ttgcacccag ttgcgatgga ggttcggccc cgagagtttc
     2761 ttgatgcgtg aaagcggccg tcgctcgatc tcgccgaact gcgaaaaatc gatctgcggc
     2821 caggccggca atccgccgcc gatcgacggc cctccgccgg gggtgcgctg gagcgcgttc
     2881 ttgacgaacc cctgcacgtc ttcgcgcgtg acccggccgt gcggtccgct tccgcgcaag
     2941 ccgcgcaact cgacgccgag ttcgcgcgcg aaccggcgga tagccgggct cgcgtgcacg
     3001 atcccgtcgc cggtcgacgg cgccggcggc aacgcagccg cggcagccgg tgcggtttcg
     3061 gccggtgcgg gaggcggcgc cgctgtgggc ggcgctgcgg gcggcggcgc cgctgcgggc
     3121 ggttgtccgg caaccgtcag cgtcgcgatg acgtcgcctt gcgacacctt gtcgccggct
     3181 ttgagccgga cgtcggcaat cgtaccggcg gcggcggccg ggacttccat cgaagctttt
     3241 tcgctctcga gcacgagcag cggcgcttcc gccgcgaccg tttggcccgg tctcaccagc
     3301 acttcgacga ccggcacgtt tttgaaatcg ccgatgtcgg gaaccttcag ttcgatcgta
     3361 gccggcgccg gagcggccgc ggatgcggga gcgggtgcgc ccccggacgg cgcggcggtc
     3421 gcatcccgca acgtgaggat cacgcagccc tccgacacct tgtcgccgac gcggacgttg
     3481 acgccgtcga ccgtgccggc cgccgtcgac gggacttcca tcgacgcttt ctcgctctcg
     3541 aggacgatca gcggcgtctc cttcgcgatt gcctgtcccg gtttcaccaa gacctcgatc
     3601 accggcacgt ctttgaagtc gccgatgtcg ggtacgcgga cttcgatggc gctgttcacg
     3661 tcgtaatcgg gttgggcttt tcgggatcga tgcgatactt tttaatcgcc ttgctcacca
     3721 tcgaggccgc gacggtccct tcgtcggcca gcgctttgag cgccgcgagc gtcacgaagt
     3781 gccggttgac ttcgaaaaac tcgcgcagtt tgcggcggta gtcgcttcgc ccgaaaccgt
     3841 cggtcccgag cgtgacgtag cgccgtccga cgaacggatg gatctgctcg gcgaaggtgt
     3901 gaatgtagtc ggtcgcggcg atgcccggtc ccgggtggcc gtcgagcgac gcctcgacga
     3961 acgagcggcg cggcggtgct tcggggtgca gcaggttcca gcggcgcgtc gcgatcccgt
     4021 cgcgttgcag ctcgttgaag ctcgtgacgc tccagacgtc gctgcgcacg ccccattcgt
     4081 tttccaataa gtcggccgcc gccagcactt cgcgcagaat cgctcccgag ccgaacagct
     4141 gcacgctcgg tgccttcttc tccgcggcgg cgcccgcccg cagcaagtac attcctcgaa
     4201 ggatcccctc acgcgcgttc tccggcatcg gcgggtgcgc gtaattttcg ttcagcagcg
     4261 tgatgtagta gtagacgtct tcctggtcgg ccagcatccg ccgcagcccg tcttgaatga
     4321 tcgtggcgac ttcgtagttg taggtcggat cgtagctgcg gcagttcgga acgaacgatg
     4381 ccgccacttg gctgctgccg tcttcgtgct gcagcccctc gccgttgagc gtggtgcgcc
     4441 ccgacgttgc ccccagcagg aagccgcggg tccggctgtc gcccgccgcc cacgtaaggt
     4501 cgccgacgcg ttgaaatccg aacatcgaat agaagatgta gaacgggatc atcggcagat
     4561 tgtgattgga gtacgacgtc ccggccgcga tccacgacga gagcgccccg gcttcgttga
     4621 tgccctcttg caaaatctgg ccgagcttgt cttcgcggta ccacatcagc tgctcggcgt
     4681 cttgcggatg gtagagctga ccgaccgacg agtagatgcc cagctggcgg aacatccctt
     4741 ccataccgaa cgtgcgcgat tcgtccgcta cgatcggcac gacgcgcggc ccgacggccg
     4801 cgtcgcggac gatcgtgttg aggatgcgca cgaacgccat ggtcgtcgag atttcacggt
     4861 cgccggtgcc ttgcagctgc tgctcgaagg ccgaaagttc gggcaccgcc agcgaggccg
     4921 actttcggcg ccgctgcggc acgcgtccga gcgtttgctc gcgctcgcgc aggtagcgca
     4981 tctccgggct gtcttcggcc ggcttgtaga acggaatttt ctcgatttcc gcgtccgaga
     5041 gcgggatcgc gaagcggtcg cgaaacgcgc gcatcgcctc gacctccatc ttcttggctt
     5101 gatgcgcgat attgcgcgct tcgccggccg cgcccatccc gtagcctttg atcgttttcg
     5161 cgagaatgac ggtcggctgg ccgcgatgtt cgaccgcgcg ccggtaggcg gcgtacactt
     5221 tcggcgaatc gtgtccgccg cgctgcagcg cccaaatttg atcgtccgtc cagtcggcaa
     5281 cgagcgccgc cgtctccggg tatcgcccaa aaaacgcttc gcgcacgtac ttcccgtcgc
     5341 gcgatttgaa cgtttgatag tccccgtcga cgcactcgtt catcagctgg acgagacgcc
     5401 cgctaccgtc ctcgcgcagc agccgatccc aactgcttcc ccagatcacc ttgatcacgt
     5461 tccagccggc gcctttgaag acgcgctcga gttcctggat gatctttccg ttgccgcgca
     5521 ccggaccgtc gagccgttgg aggttgcagt tgaccaccca aatgaggttg tcgagtttct
     5581 cgcgccccgc cagggagatc gcgccgagcg attcgggctc gtccatttcg ccgtcaccgt
     5641 gaaaggccca gacctttcgg tcgcgcgcgt cgatcagtgc ccggtcgtgc aggtagcgca
     5701 tgaagcgggc ctgatagatc gacataatcg ggccgagccc catcgagacc gtcgggaatt
     5761 gccagaattc cggcatgagc cagggatgcg ggtacgacga gaggccttgc ccgtcgactt
     5821 cctggcgaaa gtgatcgagc tgcgactcgg tgattcgccc ctcgagaaac gcgcgcgcgt
     5881 aaaacccggg ggcggagtgc ccttgtaaga agaccagatc tccgccgaac gtttccgacg
     5941 gcgcccggaa gaagtggttg aatccgacgt cgtacagcgt cgcggcggac gcgaagcttg
     6001 cgagatgacc gccgagttcc gagctgacct tgttggcgcg caggacggtc gcgatcgcgt
     6061 tccagcggat gtagtgccgc aggtgcgctt cgagttcgcg atcgcccggc atcggcggct
     6121 gctcctcgac cgggatcgtg ttgacgtacg gcgtttcgag cccgagcgaa acgtgagcgc
     6181 cgccgcgctg cgcttcgtcg acgactcgtc cgatcaactc gcgcgcgcgg tcggggcctt
     6241 cgagggtcac gacgccgcgc atcgcgtcga cccactcctg ggtctcggcc ggatcgggat
     6301 cgtgcacgga tgcggtcata gttcggcgac ctttccgcgc gcccaaccaa tcccttgcgg
     6361 ctggaccggt ccaaccccaa ccctgggttc cgccaattca ggaaacgcgc tacgcgcgaa
     6421 ggcgttcatc ggaaagcacc agtccgagcg cggcgagcaa gacggcaccg ccggcgacga
     6481 ccgccggcgg cagcggttcg ccgaaaacga cgagcgcgag tatcgcggcc gatacgggct
     6541 ccatcagcgt gctgaacgcc acggcgctcg gcgagaacca gcgcagcgcc gcattgatcg
     6601 cggtgtggcc gagcaactgc gagacgagcg ccataccgag gattccgccc caggcgccgg
     6661 cggcggagag cggcggcggc ggctgatgcg cgagcgcggc cgcgagaatc agtaccgccg
     6721 ccgcccaagc gtaggtgcgc gttacgatgg cacgcgtcgg caaggctccg cgcgcctcgc
     6781 ggacgatcag gagataggcg gccatcgcga gcgcgccgcc gagcgcgagc agggctccca
     6841 ccagcggttt ggcatgtgcc gggttttcgt acgcgccgaa tccgacgacg cacgtgaggc
     6901 cgattgcccc gatgacgaac gctgcgagcg ttcgcggcga gggcgcgcgg cggtgcgcga
     6961 cggcgtcgta agcggccgtc caaaccggcg tggtcgcgac cagcagcgtc gaaacggcca
     7021 ccgacgtgtc gtcgagcgag gcgatccacc agccgaagtg cgccgccagc gcaatccccg
     7081 cggcgcccag gagcactgcc tgccggcgcg tcggccgcgc gctcgcatcg cggcgaagcg
     7141 aggcgatcgc gagcaaaacg agtgcggcga tgcacaggcg cgaggccgca accgcgatcg
     7201 gcccggcccc ggtgagcgcg aagcgtgcga aaatggccgc cgcaccgacg gccagctggg
     7261 cgccgccgag caacagatac gggcccgcct gcggttgcca tttcgtcggt gtgcgcgtgc
     7321 ccgtggtttc cgcagcgtgg gtgcgttccc tagggaattg tgcggcggta ttgctaagta
     7381 gcgtgcagac gggagctcgc gaacga
//
LOCUS       DASUCJ010000092         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_31574, whole genome shotgun sequence.
ACCESSION   DASUCJ010000092 DASUCJ010000000
VERSION     DASUCJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31574"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /gene="gcvT"
                     /locus_tag="VFU90_01080"
     CDS             complement(<1..1056)
                     /gene="gcvT"
                     /locus_tag="VFU90_01080"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5478387.1"
                     /translation="MTAPAQSLRRTALHDEHVALGARMVPYAGFAMPVQYAGILQEHD
                     AVRNRAGLFDLSHMGQFDMTGERVAEWADTLAINAVATMKPLQARYNIFCNERGGTHD
                     DTIFYRLDGRWLLVVNAANADKMWAHLNAHAPASGVRLDNRHGERALIAIQGPASVAM
                     LQPHVGADLSAMKYYFCVEDRVDGVPAVIARTGYTGEDGFEMFVENEAAARIWRTLLR
                     EHSAEGLRPCGLGARDVLRLEAGMPLYGHELTEEITPVQAGQTWALKLGKPQFIGREA
                     LAAQLEAGDFSRIVGLVMEGRAPAREGYPVWWQERLAGEIRSGSPAPSVDNKNVATAL
                     VPRDAATPGSVLEVEIRG"
     gene            complement(1053..1502)
                     /locus_tag="VFU90_01085"
     CDS             complement(1053..1502)
                     /locus_tag="VFU90_01085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5478388.1"
                     /translation="MAIDYDHAPHAWEKERGNFVLLLDLKLEKVERGMAVMRMPFRQE
                     ITNLSGGVHGGAIVSLCDTAFYVALASIYGREQDTTTVALQCNFLAPARPPHDLVAEA
                     RVLKAGRRVVYGEVNVRSGDKLVAHATLNFLNTYAEEKPQSAHGAKR"
     gene            complement(1534..2358)
                     /locus_tag="VFU90_01090"
     CDS             complement(1534..2358)
                     /locus_tag="VFU90_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5478389.1"
                     /translation="MSARRFDGKTAVVTGGGTGLGRAMAEAFAAEGAAVAVIGRRREK
                     LDEAVAAIEAGGGTAFGLPFDVRDPAAAEAGMAEIVRRTGRLDVLVNNAAGNFVCPSE
                     ELSPNGFKSVVDIVLLGTFYCTRYAFAALRAGGGSVLNVVAAYAWSGEPGAVHSASAK
                     AGVLAMTRTLAAEWGRYGIRVNAIAPGPIHTEGTDKNLWSVGNVEEAVRASVPLGRLG
                     EPGDVARAALWLSSDEAAWISGATLSVDGAQWLHGGTLDFRAAYDALRSSGAGRPG"
     gene            complement(2355..>2740)
                     /locus_tag="VFU90_01095"
     CDS             complement(2355..>2740)
                     /locus_tag="VFU90_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478390.1"
                     /translation="SPSNPLFNDGVFALIKSRLSDGGVYVLQASTASFHNASLHCKMA
                     RTLRRHYAHVASFVTHVPSFDADWAFLACSDRIDLAALEPAAIDSYCATLRGENFFYD
                     AQTHRRIFSLPLYLRRALAAAGDTF"
BASE COUNT          440 a          954 c          894 g          452 t
ORIGIN      
        1 gcctcgaatc tcgacctcga gcaccgagcc cggggttgcg gcatcgcgtg ggacgagggc
       61 cgtcgcaacg ttcttattgt ccaccgacgg cgcgggcgaa ccgctgcgaa tctcgcccgc
      121 gagacgctct tgccaccaca cggggtaccc ttcccgcgcc ggcgcccggc cttccatcac
      181 cagccccacg atgcgcgaaa agtcgccggc ctccagctgc gcggccaacg cctcacgccc
      241 gataaactgc ggcttgccga gcttgagcgc ccacgtttgt ccggcctgca ccggcgtgat
      301 ctcttcggtg agctcgtggc cgtacagcgg catcccggct tccaggcgca acacgtcgcg
      361 cgcccccaaa ccgcagggcc gcagcccctc ggccgagtgc tcgcgcaaca gcgtccgcca
      421 aatccgggcg gccgcctcgt tctcgacgaa catttcaaaa ccgtcttcac cggtgtatcc
      481 ggtacgcgcg ataaccgcgg gaacgccgtc cacgcgatcc tccacgcaga agtagtactt
      541 catcgcggag aggtcggcgc cgacgtgcgg ctggagcatc gcaaccgacg ccggtccctg
      601 aatggcgatc agcgctcgct ccccgtggcg gttgtcgagg cgcacgcccg acgcgggtgc
      661 gtgggcgttg agatgcgccc acatcttgtc ggcgttggcc gcgttgacca ccagcagcca
      721 gcgcccgtcg agccggtaga aaatcgtgtc gtcgtgcgtg ccgccgcgct cgttgcaaaa
      781 gatattgtag cgcgcctgca gcggtttcat ggtggcaacc gcattgatgg ccagcgtatc
      841 ggcccactcc gcgacccgct cgccggtcat atcgaattga cccatgtgcg agaggtcgaa
      901 cagcccggca cggttgcgca cggcgtcgtg ttcctgcagg atgcctgcgt actgaacggg
      961 catcgcgaac cccgcgtacg ggaccatgcg cgcgccaagt gcgacgtgtt cgtcgtgtaa
     1021 ggcggtccgg cgcaaggatt gagccggcgc ggtcatcgct tggcgccgtg cgcggactgc
     1081 ggtttttctt ccgcgtacgt gttcagaaag ttgagcgtcg cgtgagcgac gagtttgtcg
     1141 ccgctgcgca cgttgacttc gccgtaaacg acgcgccgcc cggccttgag cacgcgcgct
     1201 tcggctacca gatcgtgagg cggccgagcc ggtgccagga agttgcactg caacgcgacg
     1261 gtcgtggtat cttgctcgcg gccgtagatc gacgccaacg cgacgtagaa cgccgtgtcg
     1321 cacaggctga cgatcgcgcc gccgtgcacg ccgccgctga ggttggtaat ctcttggcgg
     1381 aacggcatgc gcatcaccgc catcccgcgc tcgactttct ccagttttaa gtcgagcagc
     1441 aggacgaagt ttccgcgctc tttttcccac gcgtgcgggg cgtggtcgta atcgatggcc
     1501 atgtcggcgg ggtgtttccg ggcaggaacg cctctatccc ggtcgtcccg cgcccgagga
     1561 ccggagcgcg tcgtacgccg cgcggaagtc gagcgtaccg ccgtgcagcc actgcgcccc
     1621 gtcgaccgaa agcgtcgcgc cgctgatcca cgccgcttcg tccgacgaca gccacagcgc
     1681 cgcccgcgcc acgtcgccgg gctctcccag gcggccgagc gggaccgacg cacgcacggc
     1741 ttcttccacg ttgccgacgc tccataggtt tttgtcggta ccctcggtat gaatcggccc
     1801 cggcgcgatc gcgttcacgc gaatgccgta acgcccccac tccgccgcca gcgtgcgcgt
     1861 catcgccagg acgcccgctt tcgcggacgc cgagtggacg gcccccggct cgccgctcca
     1921 cgcataggcg gcgacgacgt tgaggacgct gccgccgccg gcgcgcagcg cggcgaacgc
     1981 gtaacgcgtg cagtagaacg ttccgagcaa gacgatgtcg acgacgcttt tgaagccgtt
     2041 gggcgaaagc tcttcggacg ggcagacgaa gtttccggcg gcgttgttga cgagcacgtc
     2101 gagccggccg gtgcggcgca cgatttcggc catcccggct tcggccgctg cggggtcgcg
     2161 aacgtcgaac ggcaatccga aggcggtccc gccgccggct tcgatcgccg cgacggcctc
     2221 gtcgagtttt tcgcggcgcc gtccgatcac cgcgaccgcg gcgccttcgg ccgcgaacgc
     2281 ctcggccatc gcgcgcccga gtcccgttcc tccgccggtg acgaccgcgg tttttccgtc
     2341 gaagcggcgc gcgctcaaaa cgtatccccg gcggcagcga gcgcgcgtcg cagatagagc
     2401 ggcaacgaaa aaatccgccg gtgggtctgc gcgtcgtaga agaaattctc gccgcgcaag
     2461 gtcgcgcagt acgagtcgat cgcggccggc tccagcgctg cgaggtcgat gcggtcgctg
     2521 cacgccaaga acgcccagtc ggcgtcgaac gagggtacgt gggtcacgaa cgacgcgacg
     2581 tgcgcgtagt gccgccgcag cgtacgcgcc atcttacagt gcagcgacgc attgtgaaac
     2641 gatgcggtgc tcgcctgcag cacgtacact ccgccgtcgg acaaacgcga ttttatcagc
     2701 gcgaaaacgc cgtcgttgaa gagcggattg ctgggcgaat
//
LOCUS       DASUCJ010000093         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1086107, whole genome shotgun sequence.
ACCESSION   DASUCJ010000093 DASUCJ010000000
VERSION     DASUCJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086107"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..>1054)
                     /locus_tag="VFU90_01100"
     CDS             complement(358..>1054)
                     /locus_tag="VFU90_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478391.1"
                     /translation="LGIIQPSPESPMNIVRPLHLCCAVPVLAAIVVAAACSSSSSTGP
                     NTGQNVRILTRFDSLRGTTADGQRASEFQSIAQILAEGAPLGAGLIKNGAVTGRFTMV
                     AALLVDDVNGQPVDSQYTVIGYEGNAPDSVVLFNASSSGVFSIVSDHDGSVGTIGGTV
                     ALTPDAPGAACKSFLAQAPSDLDAPAPITCRLQTVTASFVTAVGTSADTLSLPNQKVS
                     GIRIETAAAPAAP"
BASE COUNT          182 a          340 c          375 g          157 t
ORIGIN      
        1 ccgtcacgga ttcactggag cacgcaaccg cgaccatggg cgcgatgagg atggcgagca
       61 cgcacggcgt gtgaagcgaa cgcggcagat gcatggctgg tgccctccgg caggcaaacg
      121 gacatcgttg gtcgcgcacg atgctcccgc gcatcgacgt atcctgcgga agctggacca
      181 caaatccatg gctcgccaga gcggttccac gcgttagacc gtaaggcacg tgcgcgattt
      241 gatcggacaa tatgggcgcg accgcgacgg cacccgcgtg ccgcccaaca cggaccatgc
      301 ggccctcggc cgatcgttgg gcgtccggtg cccaacggtc ggccgaggcc taacgcatca
      361 cggcgcggcc ggcgcggccg cggtttcgat ccggatgccg gataccttct ggttcggcag
      421 cgagagggtg tcggcgctcg taccgaccgc agtgacaaag gacgcggtga cggtttgcag
      481 ccggcacgtg atcggcgccg gcgcgtccag gtcggacggc gcctgggcga ggaacgactt
      541 gcacgccgcg ccgggtgcgt cgggcgtcag cgcgaccgtg ccgccgatcg tgccgaccga
      601 gccgtcgtga tcggagacga tcgagaacac cccactgctc gacgcgttga agaggacgac
      661 gctatcgggc gcgtttccct cgtacccgat caccgtgtac tgggagtcga cgggttggcc
      721 gttcacgtcg tccacgagca acgctgccac catcgtgaag cgccccgtca cggcgccgtt
      781 cttgatgagg cccgcaccga gcggcgcgcc ctcggcgaga atctgcgcga tgctctggaa
      841 ctcgctggca cgctggccgt cggcagtggt gccgcgcagg gagtcgaagc gcgtgaggat
      901 gcgcacgttc tggcccgtgt tcggaccggt ggacgacgaa ctggagcacg ccgctgcgac
      961 gacgatcgcg gcgaggaccg gcacggcgca gcacagatgg agcggacgaa cgatgttcat
     1021 ggggctctcc ggcgatggtt ggataatgcc taac
//
LOCUS       DASUCJ010000094         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_321925, whole genome shotgun sequence.
ACCESSION   DASUCJ010000094 DASUCJ010000000
VERSION     DASUCJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321925"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFU90_01105"
     CDS             <1..284
                     /locus_tag="VFU90_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5478392.1"
                     /translation="HRAIKPQCERYARLYASVLLDELPTSIKEHEAIASAIARGDADA
                     AQVAAETNWANAASRLAHIIWQHGERGSWVGPREGSAPAPRKLRRRNSA"
     gene            525..>1521
                     /locus_tag="VFU90_01110"
     CDS             525..>1521
                     /locus_tag="VFU90_01110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5478393.1"
                     /translation="MPPVDTAGPPHASLDDTAQLGAFLDGAFAQFIDQLHVPSAVVVV
                     IKDGRVLYTHGYGFADVDKKTPINPATSLFRIGSTSKLFTWTAVMQLVQQGKLDLDAD
                     VNTYLTDFKIPATYAKPVTLRSLMTHSPGFEDGALGYLISNDSASVKSIDETLKEHMP
                     ARVRPPLVLSSYSNYGAALAGLIVQNVSGMPFNDYIQKNILDPLDMHHTTFQEPLPAN
                     LKPDAVTGYVYANGVYEPKPFEFVGGFRPAGSVSASALDMTHFMIAHLQDGRYGDTRI
                     LDSATAERMHTRTFANDPRLPAMALGFYEQKMGGVRVIGHEGDTQYFHTAMFIVPT"
BASE COUNT          283 a          546 c          444 g          248 t
ORIGIN      
        1 tccaccgcgc gatcaagccg cagtgcgagc gctatgcgcg actctatgcg agcgtcctcc
       61 tcgatgagct gccgacgtcg atcaaggagc acgaagcgat cgccagcgcg attgcgcgcg
      121 gcgatgccga tgcggcgcag gtcgccgccg agaccaactg ggcaaacgcg gcatctcggc
      181 tcgcacacat catctggcag cacggtgagc gcggcagttg ggttgggccg cgcgaaggct
      241 ccgcacccgc tccccgcaag ctccgccgcc gaaacagcgc ctgagttccg acccacgggt
      301 tccgaggggt ggacactcgg aaccgtgcgc ggcgacgtct tgcggtaaaa cggtataccg
      361 atactgtata ccagttgacc cgctcccctc ttcttgacgg agactcgtcg cccatgcacg
      421 cctaccgcgg aacccgcacc cgcgccctcc tccttctcgt tgccgctgcc gccctctcga
      481 tcgcggcatc cgcgcacgcg caaaaatcgg ctcgagccga gcgcatgccg ccggtcgata
      541 ccgccggccc tccgcacgcc tccctcgacg ataccgccca actcggtgcc ttcctcgatg
      601 gcgcgttcgc gcagttcatc gatcaactcc acgtcccgag cgcggtcgtc gtcgtgatca
      661 aggacggacg cgtcctctac acccacgggt acggattcgc ggacgtcgac aaaaaaacgc
      721 cgatcaatcc tgcgacgagc ctcttccgca tcgggtccac ctccaagctg ttcacctgga
      781 ccgcggtgat gcagctcgtg caacagggga agctcgacct cgatgccgac gtcaacacct
      841 acctcacgga tttcaagatt cccgccacgt acgcgaagcc ggtcacgctg cgcagcctga
      901 tgacgcactc gccgggcttc gaagatggtg cgttaggcta tctcatcagc aacgattcgg
      961 cgagcgtcaa atcgatcgac gagacgctca aggagcacat gccggcccgc gtgcgtccgc
     1021 cgctggtgct gtcctcctat tccaactacg gcgcggcgct cgccgggctc atcgtgcaga
     1081 acgtgagcgg catgccgttc aacgactaca tccagaagaa cattctggac ccgctcgaca
     1141 tgcaccacac cacgttccag gagccgctgc cggcgaacct caagccggac gccgtcaccg
     1201 gatacgtcta cgcgaacggg gtgtacgagc cgaagccgtt cgagttcgtc ggcggcttcc
     1261 ggcccgccgg ctcggtgtcc gcatcggcgc tcgacatgac gcacttcatg atcgcgcacc
     1321 tgcaagacgg ccggtacggc gacacccgca ttctcgactc tgcgacagcc gaacggatgc
     1381 acacgcgcac cttcgcgaac gatccgcgtc tgccggcgat ggcgctcggg ttctacgagc
     1441 agaaaatggg cggcgtccgc gtgatcgggc atgaaggcga cacgcagtac ttccacacgg
     1501 cgatgttcat cgtgccgacc g
//
LOCUS       DASUCJ010000095         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_459559, whole genome shotgun sequence.
ACCESSION   DASUCJ010000095 DASUCJ010000000
VERSION     DASUCJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459559"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFU90_01115"
     CDS             <1..678
                     /locus_tag="VFU90_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478394.1"
                     /translation="FLFAVREGPYQAPSATVSLAGLRRVVGDRGVILATAGYLGHMWE
                     LYAMWSWIGLFWTAVALQHALPRVVPSIMAFATVASGAIGCVVAGLFADRFGRTTTTM
                     TAMVISGCCALGVGVAYRAAASPMVLGVITLIWGVSIVADSAQFSACVTELAPPEYVG
                     TALTVQTCLGFLLTVASIRAVPVWVAQWGWGYAFAPLAVGPLFGTLAMFRLRAAQYRA
                     RLAGGHG"
     gene            689..>1569
                     /locus_tag="VFU90_01120"
     CDS             689..>1569
                     /locus_tag="VFU90_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478395.1"
                     /translation="MSGPSARADLLTSRRRRLGSSLTGGTIAAVVAALSLILLWIALR
                     MAGFDATLAMGALWRGSFGAWYALTSSTLVRATPLILTGLAVAIAFRAGVWNIGADGQ
                     LLIGAALATAVGLAWGDALGWAALPIALIAGAAGGACWAGIAAVLRTRFGVLEVISTI
                     MLNFVALDLVGYLVRGPLQEPSHAYPQSKPIPEHMDLPFLIPGTRLHWGFALGVAAAI
                     VLWWAMRSTAAGFRVRAIGANADAAHIAGQIDVARTTTNVLLLSGALAGLAGAIEVSG
                     VTYALYENLSPGYGYTA"
BASE COUNT          186 a          506 c          557 g          320 t
ORIGIN      
        1 ttcttgttcg cggttcgcga gggcccgtat caagcgccgt cggcgaccgt gagcctcgcc
       61 ggactgcgac gggtcgtcgg cgatcgcggt gtgatcctgg cgaccgcagg ctacctcggc
      121 cacatgtggg agttgtacgc catgtggagt tggatcgggt tgttctggac cgcggttgct
      181 ctccaacacg cgctgcctcg tgtggtaccg agcatcatgg cgttcgccac ggtcgcgtct
      241 ggcgcgatcg ggtgcgtggt tgccgggttg ttcgcggatc gcttcggtcg tacgacgacg
      301 acgatgaccg ccatggtgat cagcggatgt tgtgcgttag gcgtcggcgt tgcctatcgc
      361 gcggcggcgt cgccgatggt cctcggcgtg atcacgttga tctggggcgt gagcatcgtg
      421 gccgactccg cacagttctc ggcctgtgtg acggagctcg cgcccccgga gtacgtggga
      481 accgcactca ccgtgcagac ctgtctcggc tttctgctca cggtcgcgag catccgggcc
      541 gtgccggttt gggttgcgca gtggggctgg ggctacgcgt ttgcgcctct cgcggtgggc
      601 ccgctcttcg gtactctcgc gatgttccgg ctgcgggccg cgcagtatcg cgcgcgtctc
      661 gccggcggtc acggttaggc agtggcacgt gtccggcccc agcgcccgag cggacctttt
      721 gacgagtcgg cggcggcggc tcggctcgtc gctgacgggc gggaccattg ccgctgtcgt
      781 tgccgcgctc tcgctcattc tgctgtggat cgcgctgcga atggccggct tcgatgccac
      841 gctcgcgatg ggtgccctgt ggcgtggctc attcggggcc tggtacgcac tcacgtcgtc
      901 cacactcgtc cgtgcgacgc cgctcattct cacggggctt gcggtcgcga tcgcatttcg
      961 cgccggcgtc tggaacatcg gcgcggacgg gcagctgctc atcggagccg ctctcgcgac
     1021 ggccgtcggc ctcgcatggg gtgacgcgct gggatgggcc gcgctcccga tcgcgctcat
     1081 cgccggcgcc gccggaggcg cgtgttgggc gggcatcgcc gcggtgcttc gcacgcgctt
     1141 cggcgtgctc gaggtgatca gcaccatcat gctgaacttc gtcgcactcg atctcgtggg
     1201 gtacctggtg cgcggtccgc tgcaggaacc gagtcacgcg tatccccaga gcaaaccgat
     1261 tccggagcac atggacttgc cgtttctcat tcccgggaca cggctgcact ggggatttgc
     1321 gttaggcgtc gccgcggcga tcgtgctctg gtgggccatg cgatcgactg ccgccggatt
     1381 tcgcgttcgt gcgatcggcg ccaacgccga cgccgcgcac atcgcgggac aaatcgacgt
     1441 cgcccggacc acgaccaacg tgttgttgct gagcggcgcg ctcgccggct tggccggcgc
     1501 gatcgaagtc agcggtgtca cgtacgcgtt gtacgagaac ctttcgccgg gctatggcta
     1561 cacggcgat
//
LOCUS       DASUCJ010000096         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_841623, whole genome shotgun sequence.
ACCESSION   DASUCJ010000096 DASUCJ010000000
VERSION     DASUCJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841623"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFU90_01125"
     CDS             <1..644
                     /locus_tag="VFU90_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5478396.1"
                     /translation="RRPDARPTRLSGAGRRSRYAPITRRSLINDKESWMKPILKPLQL
                     AAIAVLAATSLAVQAQGAGPTDPQIAAIVVTANQVDIDAGKLAQARSHNKDVRAFGKM
                     MVTDHTGVNKSAVALVTKLHVKPEDNPTAESLKKEGEENIAHLKTLKGQAFDTAYIDH
                     EVTYHQQVIDAMDKTLIPSAQNAQLKALLVKVRPAFVAHLNAAKELQAKLEKK"
     gene            647..1003
                     /locus_tag="VFU90_01130"
     CDS             647..1003
                     /locus_tag="VFU90_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEU5478397.1"
                     /translation="MRALQVTDNGRRRFLAAAAAVAAAAATLRAGRSLAAPAVHTVVI
                     DGFAFKPAELTVNRGDTVVWKNNDPVPHTATAKGVFDSGSIPAGGSWKYVATKRGRFD
                     YICTFHTIMKGTLVVR"
     gene            complement(1052..>1409)
                     /locus_tag="VFU90_01135"
     CDS             complement(1052..>1409)
                     /locus_tag="VFU90_01135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5478398.1"
                     /translation="GTLVGHYRHRVHADPFLWPGLSDLTTHVDFTALAEAAEGAGLVV
                     AGFATQASFLLGCGILDRLAATGAPESLAYLREAAAVQKLTSPAEMGELFKVLLIAPA
                     SHSWRSLAVTDLTHRL"
BASE COUNT          272 a          479 c          453 g          205 t
ORIGIN      
        1 ggcgccgccc tgatgcacgc ccgacccggc tatccggtgc cggccgacgt tcgcgctatg
       61 cgccgatcac gcgccggtcc cttatcaacg acaaggagtc atggatgaaa ccgattctga
      121 aaccgcttca gctcgccgca attgccgttc tcgccgccac ctcgctcgcc gtccaggctc
      181 agggggccgg tccgaccgat ccgcagattg ccgccattgt cgtcaccgcc aaccaggtcg
      241 acatcgacgc cggcaagctc gcgcaggcgc gctcgcacaa caaggacgtg cgcgcattcg
      301 gcaagatgat ggtcaccgac cataccggag tcaacaagtc cgccgtcgcg ctggtgacga
      361 agcttcacgt caagcccgag gacaacccta ccgccgaaag cctgaagaag gagggcgagg
      421 agaatatcgc ccatctgaag acgctcaagg ggcaggcgtt cgacacggcc tacatcgacc
      481 atgaagtcac ctaccatcag caggtgatcg acgcgatgga caagacgctc attccgagcg
      541 cgcagaacgc ccagctgaag gcgctgctgg tgaaggtgcg tcccgcgttc gtcgcccacc
      601 tcaacgcggc gaaggagctg caggcgaagc tcgagaagaa atagtcatgc gcgcgttgca
      661 ggtcaccgac aacggccgcc gtcgcttcct cgcggcggcg gctgccgtcg cggcagccgc
      721 ggcgacactg cgtgcgggcc gctcgctcgc agcgcccgcg gtccacaccg tcgtcatcga
      781 cggcttcgcc ttcaagcccg ccgagctcac agtcaatcgc ggcgacaccg tggtgtggaa
      841 gaacaacgac cccgttccgc acacggcgac cgcgaagggc gtgttcgatt cgggctcgat
      901 tcccgccggc ggatcgtgga agtacgtcgc gaccaaacgt ggccgcttcg actatatctg
      961 cacgttccac acgatcatga aaggcacgct tgtcgtgcgc tagggtccgt caacgctgcg
     1021 cctgcaggcg taaacgcgac ccggtgcgcg atcacagccg atgggtcaga tcggtgaccg
     1081 cgaggcttcg ccacgaatgc gacgcgggcg cgataagcag caccttgaag agctcgccca
     1141 tctcggcggg tgacgtgagc ttctgcaccg cggcggcctc gcgcaggtag gcaagcgact
     1201 cgggtgcccc ggtcgctgcg aggcgatcga ggatcccgca acctagcagg aacgaagcct
     1261 gggtcgcgaa gccggcgacg acgaggcccg cgccttccgc cgcttcggcg agcgccgtga
     1321 aatcgacgtg cgtcgtgaga tccgagaggc cgggccacag gaacggatcg gcgtggacgc
     1381 gatgccggta gtggccgacc agcgttccg
//
LOCUS       DASUCJ010000097         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1284737, whole genome shotgun sequence.
ACCESSION   DASUCJ010000097 DASUCJ010000000
VERSION     DASUCJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284737"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFU90_01140"
     CDS             complement(<1..396)
                     /locus_tag="VFU90_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478399.1"
                     /translation="MTPEADLSAVRTVPIARRPNKVRAEEFAAPPREDVAGERSFATF
                     LESLPDVLVARDFRRVIDAIVSAAGRRAVIVMIGGHVVKTGLAPLLLDLMRRRVITHL
                     AMNGSAAIHDYEVARWGGTSEDVAAGLRDG"
     gene            complement(393..>1002)
                     /locus_tag="VFU90_01145"
     CDS             complement(393..>1002)
                     /locus_tag="VFU90_01145"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU5478400.1"
                     /translation="EMQILGIDTVQGHPTFHSAFTVDGGFLMFKVHDVLESWFDTTTL
                     SSRRFVQKIHEVNYEKQREYDIWPEKSQMQQHGYPVMSSVSNPLDDGSFLYFVRTVPV
                     EVGKTYTFNRYFRPEKNPVVVKVLRKERVTVPAGTFDAIVIQPIIKSQGLFSEGGEAE
                     IWLSDDPRHIVVQMKAKVPVLRTLDLYLQSYQPPITETAEDR"
BASE COUNT          180 a          314 c          316 g          192 t
ORIGIN      
        1 gccgtcgcgc agaccggcgg cgacatcctc cgacgtcccg ccccagcgcg cgacctcgta
       61 atcgtggatg gcggcggagc cgttcatcgc caggtgcgtg atgacgcgcc gccgcatgag
      121 gtcgagcagc agcggcgcga gcccggtttt cacgacgtgt ccgccgatca tcacgatcac
      181 cgcgcgccgc cccgcggcgg acacgatggc atcgatcaca cggcggaaat cgcgggcgac
      241 gagtacgtcg ggcaatgatt cgaggaatgt cgcgaacgag cgctcgccgg ctacgtcctc
      301 gcgaggcggc gccgcgaact cctcggcgcg cactttgtta ggccggcgtg cgatcggaac
      361 cgtgcggacg gccgacaagt cggcctccgg cgtcaccggt cctccgcggt ctccgtgatc
      421 ggcggctggt acgattgcag gtagagatcg agggttcgca acacgggcac cttcgctttc
      481 atctgcacaa caatgtgccg tggatcgtcg ctgagccaga tctcggcttc gccaccctcg
      541 gaaaacaatc cctgcgactt gatgatcggt tggatgacga tcgcgtcgaa ggttccggcc
      601 ggcacggtga cacgttcctt ccgtagcact ttcaccacga ctggattctt ttccgggcgg
      661 aagtagcggt tgaacgtata cgttttcccg acttcgactg gcaccgtgcg aacgaagtag
      721 aggaacgaac cgtcgtccag cggattcgac acgctcgaca tcacgggata cccgtgctgc
      781 tgcatctggg atttctcggg ccagatgtcg tattcgcgct gcttctcgta gttgacttcg
      841 tgaatcttct gcacgaagcg acgcgacgaa agcgtggtcg tgtcgaacca gctctcgagt
      901 acgtcgtgga ctttgaacat caggaacccg ccgtccacgg tgaacgccga gtgaaacgtg
      961 gggtgccctt gcaccgtgtc gatgccgagg atctgcattt cg
//
LOCUS       DASUCJ010000098         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1514049, whole genome shotgun sequence.
ACCESSION   DASUCJ010000098 DASUCJ010000000
VERSION     DASUCJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514049"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..692)
                     /locus_tag="VFU90_01150"
     CDS             complement(177..692)
                     /locus_tag="VFU90_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478401.1"
                     /translation="MAGRSGKWETMRWGISGQPLPAGLLYGPEITAACTADNFPVCFA
                     YAIAWRESIRGESNGKWNAATVVSADGGHGLFQLTSYVPTGWSDPLTNAEAALAYWLV
                     PNVNSFYDHFSLRGDALVKAAADAFNRGYGAVSADLSAGRDPDAASTGQNYGADVLAQ
                     YHQLIAGQNPS"
     gene            886..1404
                     /locus_tag="VFU90_01155"
     CDS             886..1404
                     /locus_tag="VFU90_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEU5478402.1"
                     /translation="MHFGLYEGYVKNVNLLNERLAEIRAAGKPSGADPVYAELVRRLG
                     FEYDGMRLHEYYFDNMTSRPQPLAGGKLHDALGEAFDGFDAWKDDFAAVAGMRGVGWA
                     IAYCDTENCRITNHWINDHETGNLAGFVPIVVLDVWEHAFIKDYKPSERGKYIEAFFA
                     NVDWKRCESRLP"
     gene            complement(1401..>1779)
                     /locus_tag="VFU90_01160"
     CDS             complement(1401..>1779)
                     /locus_tag="VFU90_01160"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEU5478403.1"
                     /translation="TLVQFFFGTFDLAYARFSGRPATPPSDDVIMRGAMDAEEICAGF
                     WPGDERFPEPAFWCYAFPRPPGSENLAVRPSAAFWSDAMGEYLLRYEDVRTAPSPEAA
                     LREFFTSTYEGYARLADWASAPG"
BASE COUNT          338 a          586 c          556 g          299 t
ORIGIN      
        1 cgacgttgag ataggcgggg ccctcggcac cgccggccgc cgccgattcg tagaatgccg
       61 agacccgggt gacttcggcc cgcgtccgca aacgctgcag cgcggccaga atgttgcctt
      121 gacggtcgcc gaggttggat ccgatgccga cgtaaatctc gtgcggggtc acgggcctac
      181 gaggggttct ggccggcaat cagctggtgg tactgggcga gcacgtcggc gccgtagttc
      241 tgcccggtgc tggccgcatc gggatcgcgg ccggccgaca aatcggcgct caccgcaccg
      301 taaccccgat tgaaggcgtc cgcggcggct ttcactaggg cgtcgccgcg caaactgaaa
      361 tgatcgtaga acgaattgac gttcggaacg agccaatacg cgagcgcggc ttccgcgttc
      421 gtcagcggat cgctccagcc cgtcggaacg taactggtca gttgaaacaa tccgtgtccg
      481 ccgtcggcgg aaacgacggt cgccgcgttc cacttgccgt tgctttcgcc gcgaatgctt
      541 tcccgccacg cgatcgcata cgcaaagcac accggaaagt tatccgcggt gcaggccgcc
      601 gtgatttcgg ggccgtagag caatccggcg ggcaatggtt gacccgatat tccccagcgc
      661 atcgtttccc acttaccgga gcgcccggcc actcctgtcg cgacgcaccg gccccttcct
      721 tacccggcct ccgggacccg atctgggtaa gctgacatcc tcactcgtcg atcgataatc
      781 gaaaggcagc acccctcaat gacagttgct tcagccccta agctttacac gcacaagaaa
      841 tgggagctcg ccgggctatc cggcatctcc gacgctacgc tcgaaatgca cttcgggctg
      901 tacgaaggat acgttaagaa cgtcaacctg ctcaacgagc ggctggccga aattcgcgct
      961 gcgggcaagc cgtcgggcgc agatcccgtc tacgccgaac tcgttcgccg cctcggtttc
     1021 gagtacgacg gcatgcgcct gcacgaatac tacttcgaca acatgacgag ccggccgcag
     1081 ccgctggccg gcggcaagct gcacgacgcg ctcggcgaag cgttcgacgg tttcgatgcc
     1141 tggaaagacg acttcgccgc cgtcgccggc atgcgcggcg tcggctgggc gatcgcctac
     1201 tgcgacaccg agaactgccg catcacgaac cactggatca acgatcacga aacgggtaac
     1261 ctcgcggggt tcgttccgat cgtggtgctc gacgtctggg agcacgcgtt catcaaagat
     1321 tacaaaccgt ccgaacgcgg caagtacatc gaagcattct ttgccaacgt cgattggaag
     1381 cgctgcgaat cgcggctgcc ctaacccggc gccgaagccc agtccgccag tcgggcgtag
     1441 ccctcgtagg tgctcgtgaa gaactcgcgc agcgccgcct cgggtgaggg tgcggtgcgg
     1501 acgtcttcgt agcgcaacaa gtactcgccc atcgcatcgc tccagaacgc cgcgctcgga
     1561 cgaaccgcta ggttctccga gcccggcgga cgcgggaagg cgtaacacca aaaggccggt
     1621 tccgggaagc gctcgtcgcc gggccagaat ccggcgcaga tctcctcggc gtccattgcg
     1681 ccgcgcatga tgacgtcgtc ggagggcggt gtcgccggcc ggccggagaa gcgcgcgtac
     1741 gccaggtcga acgttccgaa gaagaactgc acgagcgtg
//
LOCUS       DASUCJ010000099         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1208552, whole genome shotgun sequence.
ACCESSION   DASUCJ010000099 DASUCJ010000000
VERSION     DASUCJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208552"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFU90_01165"
     CDS             <1..529
                     /locus_tag="VFU90_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEU5478404.1"
                     /translation="LAAFVLRVRPGKFVQTYVATFKQLSKAMVTIAAMLGLAYLMNYS
                     GMTSTLGLALAATGAAFPFFSAVVGWLGVFLTGSDTSANALFGNLQVVTANALGLNPI
                     LTASVNSAAGVMGKMISVQSIAVAVAATGMSAADESRLFRFTIKHSVLLMAVMGVAAL
                     LYAYVFTGLVPSMPK"
     gene            564..950
                     /locus_tag="VFU90_01170"
     CDS             564..950
                     /locus_tag="VFU90_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5478405.1"
                     /translation="MKIEHTAYQVQDPVALAAWYVAHLGLTIKRKQAASPFGYFLADD
                     GDAVMLEFYNNPKARVPDYRAIDPLVLHIAFRADDVATTRDRLLKAGATAEGSVTTNE
                     TGDTVAMLRDPWGIPVQLVSRVSRMI"
BASE COUNT          194 a          321 c          332 g          197 t
ORIGIN      
        1 cctcgcggcg ttcgtgctgc gagtgcgtcc aggcaagttc gtgcagacgt acgtcgcgac
       61 gttcaagcag ctgtcgaaag cgatggtcac gatcgccgcg atgctcggtc tcgcgtacct
      121 gatgaattac tcgggcatga cgtccacact tgggctcgcg ctcgccgcga cgggcgccgc
      181 gtttccgttt ttcagcgccg tcgtcggctg gcttggcgtg ttcctcaccg gcagcgacac
      241 gtcggcgaac gcgctgttcg gcaatctgca ggtggtcacg gcgaacgcgc tcggcctgaa
      301 tcccatcctg acagcgtcgg tcaactcagc agcgggcgtg atgggcaaaa tgatttccgt
      361 gcagagcatc gccgtcgccg tggccgccac tggaatgtcc gcggccgacg aaagccggct
      421 gttccgcttc acgatcaaac acagcgtgct gttgatggcg gtgatgggcg tcgccgcgct
      481 gctctacgcg tacgtgttca ccggcctcgt accgtccatg ccaaagtagc cggggcggcg
      541 cgtccgctga tagcattgtc ggcgtgaaaa tcgaacacac ggcctatcaa gtacaggacc
      601 cggttgcgct cgcagcgtgg tatgtcgcgc acctcggctt gaccatcaag cgcaagcaag
      661 cggcgtcccc cttcggctac tttctcgccg acgacggaga tgcggtgatg ctggagttct
      721 acaacaatcc gaaagcacgc gtgcccgatt accgcgcgat cgatccgctc gtactgcaca
      781 tcgcttttcg agccgacgac gtcgcgacga cgcgcgatcg attgctcaaa gcgggcgcga
      841 ccgccgaagg cagcgtgacg acgaacgaga cgggcgatac cgtcgcgatg ttacgcgacc
      901 cgtggggcat cccggtgcag ctggtcagca gggtcagtag gatgatctag atcgagccac
      961 aaagttagaa gtcaaaggtt cgaagtagtc cacggaactc gaggtcggcg atgagcagac
     1021 gatttgccgc gattctggtc gcgc
//
LOCUS       DASUCJ010000100         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1166, whole genome shotgun sequence.
ACCESSION   DASUCJ010000100 DASUCJ010000000
VERSION     DASUCJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..749
                     /locus_tag="VFU90_01175"
     CDS             201..749
                     /locus_tag="VFU90_01175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5478406.1"
                     /translation="MKKPQWRRRASTFVVDSPHMRLRVDELELPDGTVVADYYVRESE
                     GFVVVFPVPAADRIVMVRQYRYGSDAIHLELPAGGLHAGEDPRECAARELLEETGYEA
                     SRWTFVDSYYAEPVRATSRARIFLALDAHKTREPAPDPTEVLEVELVTFERFRTMLAD
                     GTIDAGHAIVAGYRALDHLNRL"
     gene            complement(761..1552)
                     /locus_tag="VFU90_01180"
     CDS             complement(761..1552)
                     /locus_tag="VFU90_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478407.1"
                     /translation="MSRCGVAVFVTFVLACAAALPAGAVTTSPTVRHLVYTFTWGTSS
                     DTEVHTSGMPDSASGGGGTGGMAATAPATQGSASGIATYGGGADDKGTITVDVLRQQP
                     DKGLIVNVSEQAQNRRSAEVATCVVYGDTTVVCDPNKKINVEELTLLRLLGDGFVDPD
                     KLDAGKHWQTQQQSASQSSTTDFTIAGNANGAMTIDETRVIKDTSGARPETSNLTGTI
                     GYDFGRGLPTKIDEYTILHSEQGEQYETIKTQTVMLLQSDSSAKP"
     gene            complement(1558..2304)
                     /locus_tag="VFU90_01185"
     CDS             complement(1558..2304)
                     /locus_tag="VFU90_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478408.1"
                     /translation="MRSRASGVAVLAALTFWLSGVPVLADQAALPRHLVYGFTFGTQS
                     DLEVHSSGIDAGGGPSGGSGMTDFTGGVGDQGTITVDLLSQQSDGGVVIKVSEQAQKT
                     RSSAAATCVVYPTTGVICDPNATINPEEMALIRFLAPAFVDPSRLDAKQHWKIESSTP
                     QYSLTSDFTIAKNAGGVMTIDENRIIHQQQPTIETTEVNATIGYDFNKSLPTSVSEYA
                     IERSQAGMGQYNTVKTQTVLQLKSDSPGGH"
     gene            2406..2606
                     /locus_tag="VFU90_01190"
     CDS             2406..2606
                     /locus_tag="VFU90_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478409.1"
                     /translation="MRFTMHPLLATFLKLTALVTIGIVALLVVAFLLKIVLVAAVIAA
                     VVAAGIFAYTLFRRRSSLPVIR"
BASE COUNT          431 a          941 c          862 g          526 t
ORIGIN      
        1 gatccgttcg agcgcccgct gctgcgcgcc ggcgtcgggt tctaccactt cgaccgcata
       61 accgccgtgc gcggccacga acgctatgcc ggcgcccatc gcgccgccgc cggctaccaa
      121 gacgaggtct tccatcgctc cgacgcttcg cgcaaggagg cccaaacgct tcgtcgtcga
      181 tatggtctgg acagactacc atgaaaaagc cgcagtggcg ccggcgcgct tcgacctttg
      241 tcgtcgattc accgcacatg cggctgcgcg tcgacgaact ggaattgccc gacggcacgg
      301 tcgtcgcgga ctactacgta cgcgaatcgg agggcttcgt cgtcgtcttt ccggtgccgg
      361 ccgccgatcg catcgtgatg gtccgccagt accgctacgg cagcgacgcc atccatctcg
      421 agcttccggc cggcggtctg cacgccggcg aagatccgcg cgagtgcgcc gcgcgcgaac
      481 ttctcgagga gacgggctac gaagcctcgc gctggacgtt cgtcgattcg tattacgccg
      541 agcccgtacg cgccacgtcg agagcgcgca tcttcctggc gctcgacgcg cacaagacgc
      601 gcgagcccgc ccccgatccg accgaagtgc tcgaggtaga gctcgtcacc ttcgaacgct
      661 ttcgcaccat gctcgccgac ggcacgatcg acgccgggca cgcgatcgtc gccggctacc
      721 gcgccctcga ccacctcaat cgactgtagc tcccgttagg ttacggtttt gcggacgaat
      781 ccgattgcag cagcatgacg gtttgcgtct tgatcgtttc gtattgctcg ccctgttcgc
      841 tgtgcaggat cgtgtattcg tcgattttcg tcggaaggcc gcgcccgaaa tcgtaaccga
      901 tcgtgccggt gagattggac gtttcgggtc gcgcgccgga ggtgtccttg atgacgcggg
      961 tttcgtcgat cgtcatcgcg ccgttggcgt tcccggcgat cgtgaagtcg gtcgtcgacg
     1021 actggctcgc gctctgctgc tgcgtctgcc agtgtttgcc ggcgtcgagc ttgtcgggat
     1081 ccacaaaacc gtcacccaaa aggcgcagca gcgtcagctc ttccacgttg atcttcttgt
     1141 tgggatcgca gacgaccgtc gtatcgccgt agacgacgca ggtcgcgacc tcggcggagc
     1201 gccggttctg cgcctgttcg gaaacgttga cgatcaaacc cttatcgggc tgctggcgca
     1261 gcacgtcgac ggtgatcgta cccttgtcgt cggcgccgcc gccgtaggtt gcgatacccg
     1321 aggcgctgcc ctgtgtggcc ggggcggtcg ctgccatgcc gccggttcca ccgccgccgc
     1381 tcgcgctgtc gggcattccc gacgtgtgca cttcggtgtc gctcgacgtg ccccacgtaa
     1441 acgtatacac cagatgccgc accgtcgggc tggtcgtcac cgcgccggcc ggcagcgcgg
     1501 ccgcgcacgc aagcacgaac gtaacaaaga ccgcaacgcc gcagcgcgac acggccctca
     1561 gtgacctccc ggcgaatcgg acttgagctg cagcaccgtc tgcgtcttca ccgtgttgta
     1621 ctgccccatt ccggcttggc tgcgttcgat cgcgtattcg ctcaccgacg tcggcagcga
     1681 cttgttgaaa tcgtagccga tcgtggcgtt gacttcggtc gtttcgatcg tcggctgctg
     1741 ctggtgaatg atgcggttct cgtcgatcgt catcaccccg ccggcgtttt tggcgatcgt
     1801 gaaatccgag gtcagcgaat attgcggcgt gctgctctcg atcttccagt gctgcttggc
     1861 atccagccgg ctcggatcga cgaaggccgg tgccaggaac cggatcagcg ccatctcttc
     1921 cggattgatc gtcgcgttgg ggtcgcagat cacgcccgtc gtcgggtaca ccacgcaggt
     1981 agcggccgcc gaagaacgcg tcttctgggc ctgctcgctc accttgatga cgacgccgcc
     2041 gtcggactgc tgggaaagca agtctacggt aatcgttcct tggtcgccga cgccgccggt
     2101 gaagtcggtc atcccggaac cgccgctcgg gccgccgccg gcgtcgatcc ccgacgagtg
     2161 cacttccaaa tcgctctgcg tgccgaaggt gaagccgtac acgaggtggc gcggcaaggc
     2221 agcctggtcg gcaagcaccg ggacgccgga aagccaaaaa gtcagcgccg caagaacggc
     2281 gacgcccgac gcgcgggagc gcatgccggc atggttaacc tgcgaacgcg gtgggacctt
     2341 cagcctatcc gggtatgaaa cacgggcgcc cccgcacggt agcaattgac gaaaactccc
     2401 ggcccatgag gttcacgatg catccgcttc tcgccacctt cctcaaactc accgcgctcg
     2461 tcacgatcgg catcgtcgcg ctgctggtgg tcgctttcct tttgaagatc gtcctggtgg
     2521 cggcggtgat cgcggcggtc gtcgccgccg gcatcttcgc ttatacgctg ttccgccgcc
     2581 gctcgagcct gcccgtcatt cgctgagcag cgcggccagc gccgcgccga ccttctcggg
     2641 ttcctcgagc atcggaaggt ggccgcttcg ctcgcacacc accaagcgcc cgcgcggaaa
     2701 ggcctgcgcg gtggcgcgcg cctcctcgag gggaatcacg gcgtcgcgct ccccggcgac
//
LOCUS       DASUCJ010000101         1306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1269649, whole genome shotgun sequence.
ACCESSION   DASUCJ010000101 DASUCJ010000000
VERSION     DASUCJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269649"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..>1306
                     /locus_tag="VFU90_01195"
     CDS             78..>1306
                     /locus_tag="VFU90_01195"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEU5478410.1"
                     /translation="MNVDTALRRRESEGRPIRVALIGAGATGRAIALQLGTPVPGIRL
                     AGIANRTAAHAERAFREAGIDRWSITDSAEKATEQLARGIPVLTDDPAVLIRSPEIDV
                     VLEVTGTVEFAAAVTLDAIAHGKHTVIVNAELDSLIGPILKARADQAGVVLTNTDGDE
                     PGVAMTLFRYVRSLGLNPVAAGNIKGMVDHYRTPETQRAFAEKNDQDAWKVTSFADAT
                     KLSMETTVLANATGFHVGRRGMYGPACANVKEMANLLPPAEMLAGGIVDYALGAAPHT
                     GAFVVVHEKNRLKQAQLAYYKMGDGPFYVFYTPFHLPHLQIASTIGRAVLLDDATVAP
                     LDGPRCEVLAVAKRDLKAGETIDGIGGFSVYGLIDNTAAARAMGALPIGLAQGAVLTR
                     SVAKDAVVTAHDVAMPA"
BASE COUNT          233 a          463 c          406 g          204 t
ORIGIN      
        1 ccgcttcccc cgcaagcact ttccgatcaa gacttgcgct ggcccttcct ggaactcgcc
       61 acctcacgcg tgacgctatg aatgtcgaca ccgcactccg ccggcgggaa tccgaaggcc
      121 ggcccatccg cgtcgctctc atcggcgccg gcgcgacggg ccgcgcgatc gctctccaac
      181 tcggcactcc cgtccccggc atccgcctcg ccggcatcgc gaaccgtacg gccgcgcacg
      241 ccgagcgcgc cttccgtgaa gcgggaatcg accggtggag catcaccgat tcggcagaaa
      301 aggccaccga gcagctcgcc cgcgggatcc cagtgctcac cgacgatccc gcggtgctga
      361 tccgctcccc cgagatcgac gtcgtccttg aagtcacggg aaccgtggaa ttcgcagcgg
      421 cggttacgct ggacgcgata gcgcacggca agcacaccgt gatcgtgaac gccgagctgg
      481 attccctcat cggaccgatc ctgaaggcgc gggccgatca ggccggcgtc gtactcacga
      541 acaccgatgg cgacgagccc ggcgtcgcaa tgaccctctt ccgctacgtt cggtcgctcg
      601 gcctcaaccc ggttgccgcc ggcaacatca aagggatggt ggatcactac cgcacccccg
      661 agacacagcg cgcattcgcc gagaagaacg atcaggacgc gtggaaggtg acttcgttcg
      721 ccgacgccac gaaactttcc atggaaacga cggtgctcgc caacgcgacg ggattccatg
      781 ttggacggcg cgggatgtac gggccggcgt gcgccaatgt gaaggagatg gcgaatctcc
      841 tgccgccggc cgagatgctc gcgggcggga tcgtcgacta tgcgcttggc gcggcgccgc
      901 acacgggcgc ctttgtcgtc gtccacgaga agaatcgtct caagcaggcg caactcgcct
      961 actataagat gggcgacggt ccgttctacg tgttctacac gccgttccat ctcccgcacc
     1021 tgcagatcgc ttcgaccatc ggccgcgccg tactgctcga cgacgcgacg gtggccccgc
     1081 tcgacggtcc tcggtgtgaa gttctggccg ttgcgaagcg cgacctcaaa gccggcgaaa
     1141 cgatcgacgg catcggcggc ttcagcgtgt acgggctcat cgacaatacc gccgcggcgc
     1201 gcgccatggg cgcgctcccc atcggtctcg cgcagggcgc ggtgctcacg cgaagcgtcg
     1261 cgaaggacgc agtcgtgacc gcacacgatg tcgcgatgcc cgccga
//
LOCUS       DASUCJ010000102         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1300154, whole genome shotgun sequence.
ACCESSION   DASUCJ010000102 DASUCJ010000000
VERSION     DASUCJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300154"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1187
                     /locus_tag="VFU90_01200"
     CDS             204..1187
                     /locus_tag="VFU90_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEU5478411.1"
                     /translation="MSKRSRRRGRRSSLVALLVAAIFGAAAVGWLLLPRQRRPPPVPH
                     RVATYETPPAAEETAAPAGPVVATEAPASPAPIATSASAAVESPTPLPTIAPAPSGQG
                     SPPGGAKLAIIVDDCGQWIDTERGFVALGIPLTMSVLPDVPYASTIAREAADAGKGVM
                     LHLPMETVSGLNPGPGKVTTEMSDAQIAEQVEADLAQVPLARGVNNHEGSKGSADSRV
                     MRDVVGVLAKRGDLFFIDSRTSAASVGEQTAQQAGVPTAARDVFLDNQASVAYTEAQL
                     REAAAIAERNGSAIAIGHPRPTTLAAVRAMIPELQAAGVQFVLAQDLVSGR"
     gene            complement(1184..1513)
                     /locus_tag="VFU90_01205"
     CDS             complement(1184..1513)
                     /locus_tag="VFU90_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5478412.1"
                     /translation="MSSKQTNGDATLCPRFHRAVELIGRRWTGAIVRVLQGGPRRFNE
                     LLAAIPGMSDRLLAERLRELEAAQIIRREVQGGSPVRVIYELTACGRELETPLDALGR
                     WAERWIG"
     gene            1593..2099
                     /locus_tag="VFU90_01210"
     CDS             1593..2099
                     /locus_tag="VFU90_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5478413.1"
                     /translation="MDTAFLIVRLIIGLGLAAHGAQKLFGWFGGYGIAGTGAFLEGLG
                     YRPGALFAVAAGLGETAGGLLTFLGLGGALGPALMVLVMLVAIGSVHVTKGFFTANGG
                     WELPSLYIAGALAVAFAGSGAYALDRLFGIAFLSSAPQVWYALAAAVLAALLNLLARR
                     PAKAVTPA"
     gene            complement(2096..2596)
                     /locus_tag="VFU90_01215"
     CDS             complement(2096..2596)
                     /locus_tag="VFU90_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478414.1"
                     /translation="MPVFPPAPSVTVTIDGRRLPEYARAFVVRGRVYAPASPLVRFVA
                     DRAWLDGPDLIVERDGRRVRLPLGPGHGGALPAAYVEVGPLVRGLGESVRYLPGSRIV
                     DVRTPAAGPVTLPSPFNAVNAVPAPRPVFTPEPVPTRRPVWTGSPLPRRTPLPAPPRR
                     PSGRSR"
     gene            2619..3413
                     /locus_tag="VFU90_01220"
     CDS             2619..3413
                     /locus_tag="VFU90_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5478415.1"
                     /translation="MARFISAFRNAALRFSRDGCAFLAQSIAFNALFAMFPLTVLVLS
                     GVTLVLPFAERRTLMFFDTFAPTLHDYIAANLQSYIYGRGITSLIALAVLLWSGKNLF
                     MGLAYALDRALGVPRGRPLVHNLALSLLMLPLVGVLLVVAIALPVLFAVGARATGFVD
                     PAFFTHILAYSVSIALVFVVTVFLYRWLPNRGVSWGFALRGASVVAFAWPAVQYAFAQ
                     YVTHVDFTRVYGALSAPLVLLLWFYCIGSIFLFGAEYSAAASESRQ"
     gene            3531..4601
                     /locus_tag="VFU90_01225"
     CDS             3531..4601
                     /locus_tag="VFU90_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478416.1"
                     /translation="MRRLFALFSICSSLVLFGAEAPPPQPTPPPAATPSAAVTPPRAV
                     ASTPPAVTTPTPAASLPAGTASVVIYPFDVQTGVDQKIGIAIAEILAQEMTAAGGLNV
                     LAVPQAVKRPDFLQYAHQQNADFYISGYVTPVGDAAAVVEQVVSVESGVILFSQTAQV
                     QSVADVASQSLLARAQILAFVNRATENIQTQPANTPQPTSTNGANMQLRGISSIVDSV
                     FHHKGAPAPSPTPVVKPDRGVIVAPVTASGSVPGSDVNNATNELFFALSRHYQTQLTK
                     TADVASSADAICGTQRNNTIASGTLTETVPAHGRTQVVFTLAIYTCFGAVLDRTVGKG
                     DGFKQAVDAAVAAYATAHPQNS"
BASE COUNT          738 a         1645 c         1485 g          815 t
ORIGIN      
        1 cacggcggag ccgatccggg cgccgcgtgc gtgggcgcgt ttaccgcgac gacgccggcc
       61 aataggataa ggccggcaag caacgatttc accacgacgc ttttgatgcc gcggcggcgt
      121 cactcctggc gaaatttctc aggtgaatct ctgaaaacca acaaacggca tgatggatga
      181 gccttccacc gaaggagcgg cacgtgtcca agagatcgcg gcgccgcgga cggcgttcgt
      241 cgctggttgc gttgctcgtt gcggcaatct tcggtgcggc tgcggtgggc tggctgctgc
      301 ttccccgtca acgccgcccc ccgccggtgc cgcatcgcgt cgcaacatac gaaacgccgc
      361 cggctgccga ggaaaccgcc gctccggccg gaccggtggt agccaccgag gcccccgcaa
      421 gccccgcccc aatcgctacg tcagcgagcg cggccgtcga gtcgccgacg ccgcttccga
      481 cgatcgcacc ggccccgagc ggccaaggct cgccgccggg aggagccaaa ctcgcgatca
      541 tcgtcgacga ttgcgggcag tggatcgaca ccgaacgcgg tttcgtcgcg ctcggcatcc
      601 cgctgacgat gtcggtctta cccgacgtcc cgtacgcgtc gacgatcgcg cgcgaagccg
      661 ccgatgccgg caagggcgtc atgctgcatt tgccgatgga gacggtatcc gggctgaatc
      721 ccggtcccgg gaaggtcacc accgaaatga gcgacgcgca aatcgcagag caggtcgaag
      781 ccgatctggc gcaagtcccg ctggcgcgcg gcgtcaacaa tcacgaaggc agtaaaggtt
      841 cggcggattc acgcgttatg cgcgacgtcg tcggcgtgct ggccaagcgc ggcgatctgt
      901 tcttcatcga ctcgcgtacg agtgccgcat cggtgggcga gcaaaccgcc cagcaggccg
      961 gcgtgcccac cgccgcgcgc gacgtcttcc tcgacaatca agcatcggtc gcctacaccg
     1021 aagcgcagct gcgcgaagcg gccgccatcg ccgagcgcaa cggcagtgcc atcgccatcg
     1081 ggcatccgcg tccgacgacg ctcgcggccg tccgcgcgat gattccggaa ctgcaagccg
     1141 ccggcgtgca gttcgttctc gcgcaggatc tcgtttccgg ccgttagccg atccagcgct
     1201 ccgcccagcg ccccagcgcg tcgagcggcg tttcgagttc gcgaccgcac gcggtcagtt
     1261 cgtaaatcac gcgtaccggc gagccgcctt gaacctcgcg acggataatt tgcgcggcct
     1321 cgagctcgcg cagccgctcg gccagcaatc ggtccgacat ccccggaatt gccgccagca
     1381 actcgttgaa gcgccgcgga cccccttgca acacgcgaac gatcgcgccg gtccaccgcc
     1441 ggccgatgag ttcgactgcg cggtggaagc gcgggcagag cgtcgcgtcg ccgttggttt
     1501 gcttgcttga catccctata ggtccatggt agcgtcacga catagcttac tataagtagg
     1561 caagcaacgc aaggtaagta tgtgaggact tcgtggatac cgcatttttg atcgttcgtc
     1621 tcatcatcgg gctcggtttg gcggcccatg gcgcgcagaa gctgttcggc tggttcggcg
     1681 gctacgggat cgccggcacc ggagccttcc tggagggtct cggctatcgg cccggcgcgc
     1741 tgttcgccgt tgccgccggt cttggagaaa cggccggcgg actgctgacg ttcctcggtc
     1801 tcggcggggc gctcggtccc gccctcatgg tgctcgtgat gctcgtcgcg atcggctcgg
     1861 tgcacgtcac taagggcttt ttcacggcca acggcggctg ggagcttccc tcgctttaca
     1921 tcgccggcgc gctggcggtg gcgttcgccg gcagcggcgc gtacgcgctc gaccggctgt
     1981 tcggaatagc gtttctctcg agcgccccgc aggtctggta cgcgctggcg gcagccgtgc
     2041 tggccgccct gctgaacctc ctcgcgcggc gtccggcgaa agccgttacc ccggcctagc
     2101 gcgatcttcc ggacgggcgc cgcggcggcg ccggcagggg cgtccggcgc ggcaacggcg
     2161 acccggtcca aaccggccgc cgcgtcggca ccggctcggg agtaaacacc gggcgcggcg
     2221 ctggaacggc gttcacggca ttaaacggcg acggcagggt gaccgggccg gcagccggcg
     2281 ttcgcacgtc cacgatgcgc gaaccgggca ggtagcgtac cgactccccg aggccgcgca
     2341 ccagcggccc tacctccacg tatgccgccg gcagcgcgcc gccgtgtccc ggtccgagcg
     2401 gcaatcgcac gcgccggccg tcgcgctcga cgatcagatc tggaccgtca agccaggcgc
     2461 gatccgcgac gaagcgtacg agcgggctcg cgggagcgta cacgcggccg cgcacgacga
     2521 acgcgcgcgc gtactccgga agtctacggc cgtcgatcgt taccgttacc gacggcgcag
     2581 gtgggaatac cggcacttcg tacgatatac cgcgtcggat ggcacgattc atttcggcct
     2641 ttcgcaacgc cgccttgagg ttttcgcggg acggatgtgc gttcttggcg caatcgatag
     2701 cgtttaacgc gctgtttgca atgtttccgc taaccgtgct cgtcctcagc ggcgtgacgc
     2761 tcgttcttcc gttcgccgaa cgccgcacgc tcatgttttt cgatacgttc gcacccacgc
     2821 tgcacgacta catcgccgcc aatctgcaga gttatattta cgggcgcggc attacgagcc
     2881 tcatcgccct ggccgtcctg ctctggtcgg gcaagaacct cttcatgggg ctggcttacg
     2941 cgctcgaccg cgcgctcggc gtaccgcgcg ggcgcccgct cgtgcacaat ctcgcactct
     3001 cgctgctcat gctgccgctc gtcggcgtcc tcttggtcgt cgcgatcgcg ctgccggtgc
     3061 tgttcgcggt gggcgcacgc gcgaccgggt tcgtcgatcc ggcgttcttc acgcacatcc
     3121 tcgcctattc cgtgtcgatc gcgctcgtct tcgtcgtcac ggtgttcttg taccgctggc
     3181 tgcccaatcg gggcgtctcg tggggcttcg cactccgcgg cgcaagcgtc gttgccttcg
     3241 cgtggccggc ggttcagtac gccttcgcgc agtacgtcac tcacgtcgac ttcacgcgcg
     3301 tttacggcgc cctctcggcg ccgctggtgc tgctgctgtg gttctactgc atcggttcga
     3361 tctttctgtt cggcgccgag tacagcgcgg cggcgagcga aagccggcaa tgacggttcg
     3421 ccgcgcaccg catcgcgagc gagcggcctg cagcgctgct cggcgctcgg cgccgcacgg
     3481 gtgagcgtgg cccctgcgcg aatagtaggc caaccgctga aaggatgaac gtgcgccggc
     3541 tcttcgcgct tttctcgatc tgctcgtctc tagtgctgtt cggcgcggaa gcaccgccgc
     3601 cgcagcccac accgcctccg gccgccacgc cgtcggccgc cgtcacgccg ccacgggccg
     3661 tcgcgtcgac gccgccggcg gtgacgacgc ccacgcccgc ggcatcactg ccggcgggaa
     3721 cggcgtcggt cgttatctat ccgttcgacg tgcagaccgg ggtcgatcaa aagatcggca
     3781 ttgcgatcgc cgagatcctc gcccaagaga tgaccgctgc gggcggtctg aacgtcttag
     3841 cggtgcctca ggcggtgaag cgccccgact ttctccagta cgcccaccag caaaatgccg
     3901 acttttacat cagcggctac gtcacgccgg tgggagacgc cgcggccgtc gtcgagcaag
     3961 tcgtcagcgt cgagagcggc gtgatcctct tttcgcaaac cgctcaggtt cagagcgtcg
     4021 ccgacgtcgc ctcgcagtcg ctgttagcgc gagcccagat tctggccttc gtcaaccgcg
     4081 caaccgagaa cattcaaacg cagcccgcca atacgccgca gccgacctcc accaatgggg
     4141 ccaacatgca gttgcgcggg atcagcagca tcgtcgattc cgtcttccat cacaaaggcg
     4201 cgcccgcccc atcgccgacg cccgtcgtca agccggatcg cggggtcatc gtcgcgccgg
     4261 tcacggcgtc gggttccgtt cccggttccg acgtcaacaa tgccaccaac gagctgttct
     4321 tcgcgctcag ccggcactac caaacccaac tgacgaaaac ggccgacgtc gcttcgtcgg
     4381 ccgacgccat ttgcggcacg caacgcaata atacgattgc ctccggcacg cttaccgaaa
     4441 ccgtgccggc ccacggccgc acgcaagtcg tcttcaccct cgccatctat acgtgctttg
     4501 gggccgtgct cgaccgtacg gtcggcaaag gcgatggctt caagcaagcc gtcgacgcgg
     4561 ccgtcgctgc gtacgcgacg gcgcatcccc aaaacagcta gttactcgaa caatcccgcc
     4621 ggcggatcga tcacgatccg tcgcgcaccg atatcgacgt cgcgcacgta cgcggcgacc
     4681 atc
//
LOCUS       DASUCJ010000103         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_31724, whole genome shotgun sequence.
ACCESSION   DASUCJ010000103 DASUCJ010000000
VERSION     DASUCJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31724"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="hpnI"
                     /locus_tag="VFU90_01230"
     CDS             complement(<1..1092)
                     /gene="hpnI"
                     /locus_tag="VFU90_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEU5478417.1"
                     /translation="MLLVRLLTAGLDVVTIAAIGYTIVAIFCIARFGREPEPAAVEDL
                     PPVSLLVPLHGEEFGLEENLRAFAQQDYPACQLVLGVASADDRALPIAQRVAAALPDR
                     DIAINVGNVPSARNPKIANVLSMMRLVRHKLLILADSDTRVTPAYVRSVTAPLHDPNA
                     GVVTCLFAGVPDGSFSSQLGAMFMNEQFIPSVLVNRLFGPPRHCLGPTNAFRSEVLAG
                     IGGFEALAPHLADDYMLGNYVAARGFRIVISRYVIQTIVSDSSLAVLWNHELRWHRTI
                     RGVQPAGYAGMFLTYPVPLALAAFLLAPQLESAAALVVAALVRIALQRVAARVLRVAS
                     APLWFILPRDLFGIAVWACGLAGNAVRWRD"
     gene            1177..>2294
                     /locus_tag="VFU90_01235"
     CDS             1177..>2294
                     /locus_tag="VFU90_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478418.1"
                     /translation="MKPNWTLTVIAYGTRQVYLKFKIRVRQWGRLPAKRGPTVLITNH
                     QHMDEGEMITGRTFMLHPWKRLVMCNSRRTFETGFIAARLPWSSRFTRGVNLSGLWSA
                     FSILPIENHLFSRPLISLAEELRAAHGDLPLDAILPAETLAELDLEGRSLSELWDFAY
                     FMRAQAWVKVAKLKQPYRREVLDNLRQTRERDIAAIVERVQAGSTFYVTPEGDFSREG
                     RMHPMRGGIVEALAPIADLWLCAVAYDPFRGRRLSMLYRVLRYDGAADVGTSLAASRP
                     ITISALLAAFLLDSSQPFALADAMRAVRERIESLPDNVFVDPDVRRDPEAAVANALAT
                     LQKRGTLTRNGGRYALTGHRTDARFPHVADMVAFQRNML"
BASE COUNT          432 a          746 c          735 g          381 t
ORIGIN      
        1 gtcgcgccag cggaccgcgt ttccggccag cccgcaggcc catacggcga tcccgaagag
       61 gtcgcgcggc agtatgaacc acagcggggc ggatgcgacg cgcagcactc gcgccgcgac
      121 gcgctgcaag gcgatgcgca ccagcgccgc gaccaccagc gccgccgcgc tttccagctg
      181 cggcgcgagc agaaaggcgg ccagcgccag cggcaccgga taggtgagga acataccggc
      241 gtaaccggcc ggctgcaccc ctcgaatcgt ccgatgccac cgcagctcgt ggttccagag
      301 caccgcgaga ctcgaatccg acacgatcgt ttgaatgacg taacgcgaga ttacgatgcg
      361 gaagccgcgg gccgcaacgt aattccccaa catataatcg tcggcgagat gcggcgcgag
      421 cgcctcgaag ccgccgatgc cggcgagcac ctccgagcgg aacgcattgg tcggtcccaa
      481 acagtgtcgc ggcggtccga agagacgatt tacgagcacc gatgggatga actgttcgtt
      541 catgaacatc gcgccgagtt gcgacgaaaa cgagccgtcg ggaacgccgg cgaacagaca
      601 ggtcactacg ccggcgttcg ggtcgtgcaa cggtgccgta acggagcgca cgtacgccgg
      661 cgtaacccgc gtgtcgctat cggccaggat caacagcttg tgccgtacga gccgcatcat
      721 cgagagtacg ttggcgatct tggggttacg cgcgctcggc acgttgccga cgttgatcgc
      781 gatgtcccga tcgggaagcg ccgccgcgac ccgctgcgcg atcggcagcg cgcgatcgtc
      841 ggcactggcg acgccgagaa cgagctgaca agcagggtaa tcctgctgcg cgaacgcgcg
      901 caagttctct tcgagaccga attcctcgcc gtgcaaaggc acgagcaaac taaccggcgg
      961 caggtcttcg acggcggccg gttcgggctc gcgtccgaag cgcgcgatgc aaaaaattgc
     1021 gacaatcgta taaccgatcg ccgcaatcgt gacgacgtcg agacccgcgg tcaggaggcg
     1081 caccagcagc acgtgacgca gttcgctaag cgcaggaggc gggcctggcc gttgcatgcg
     1141 aagaaactcg caatgatttt ggtggaacga ttctagatga agcctaactg gacgttgacg
     1201 gtgatcgcct acggtacgcg tcaggtctac ctcaagttca agattcgcgt tcggcagtgg
     1261 ggccgtttgc cggccaaacg cgggccgacg gtgttgatca ccaatcatca acacatggac
     1321 gaaggcgaga tgatcaccgg gcgcacgttt atgctgcacc cgtggaagcg tctcgtcatg
     1381 tgcaattccc gccgcacgtt cgaaacggga ttcatcgcgg cgcgcttgcc ctggagctcg
     1441 cggttcacgc gcggcgtgaa tctcagcggc ttgtggagtg ctttcagcat cctgccgatc
     1501 gagaaccacc tcttctcacg gccgctcatc agccttgccg aggagctgcg cgccgcccac
     1561 ggcgatttgc cgctcgatgc gatcttgccg gccgaaacgc ttgccgagct ggatttggag
     1621 ggccgttcgc tgagcgagct gtgggacttc gcctacttca tgcgagccca ggcttgggtc
     1681 aaagtagcga agttgaagca gccgtaccgg cgcgaagtgc tcgacaatct ccgtcagact
     1741 cgcgaacgcg acatcgccgc catcgtggaa cgcgtgcaag ccggctcgac gttttacgtc
     1801 acgcccgaag gcgacttcag tcgcgaaggc cgcatgcatc cgatgcgcgg cggaatcgtc
     1861 gaggcccttg cgcccatcgc cgatctctgg ctgtgcgcgg tcgcttacga tccgtttcgc
     1921 ggccgccggc tgtcgatgct ttaccgcgtc ctgcgctacg acggcgccgc cgacgttggg
     1981 acgtcgctgg cggcgtcgcg cccgatcacg atctcggcgc ttctggccgc gtttctgctc
     2041 gattcctcgc agccgttcgc gctcgccgat gcgatgcgcg cggtacgcga gcggattgaa
     2101 tcgctgccgg ataacgtgtt cgtcgatccc gacgtgcgcc gcgatccgga agcggccgtt
     2161 gccaacgcgc ttgcaacgct gcaaaaacgc ggcacgctga cgcgaaacgg cggacgctac
     2221 gcgctcaccg gccaccggac cgatgcgcgc ttcccccacg tcgccgacat ggtcgcattc
     2281 cagcgcaaca tgct
//
LOCUS       DASUCJ010000104         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1239096, whole genome shotgun sequence.
ACCESSION   DASUCJ010000104 DASUCJ010000000
VERSION     DASUCJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239096"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFU90_01240"
     CDS             <1..931
                     /locus_tag="VFU90_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEU5478419.1"
                     /translation="ATRPALTVVRPRFRVAGRVSAAASPERIDLSGYDPASKAGFSDK
                     KAARKVLRDDIERLAHLDDVFAAARTHAMLIVLQGMDTAGKDGIIKHVLSGVNPQGVN
                     VYGFRKPSEEELLHDWLWRESRALPERGRMAIFNRSYYEEVLVARVMPGVLKKEAAPH
                     DARQWAHRYEDINAFERHLTRSGMVVVKFFLHISRDEQRKRLCKRLETPTKMWKASDS
                     DLQAHERWDAYAHAYEEMLGHTNTAWAPWHVIPADRKWVARAVVGNVLIETLEALHLH
                     YPEPSAQRRELFERLASRLEAEGDGGSPEKANA"
     gene            945..1196
                     /locus_tag="VFU90_01245"
     CDS             945..1196
                     /locus_tag="VFU90_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478420.1"
                     /translation="MKLVESIDPATLADERLVAESNEFAIYDAGNDSYTLVHRHAGVN
                     WQGITISGDGLFRVAELLATATRALYRDIAGDLSRRRSR"
     gene            complement(1193..>2014)
                     /locus_tag="VFU90_01250"
     CDS             complement(1193..>2014)
                     /locus_tag="VFU90_01250"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEU5478421.1"
                     /translation="LSTLEVWYVQIGLVELGPFFGKNAAAAGSLHDAAIVERGRGRIV
                     DKPPLVYHFRECAPAWAGLIHTFFARYRQSISADRRQLFDRFTFEDLGVKIVGVGSVG
                     TRCTVSLFLSDDADPLFLQIKEARRSVLEPPESRSRYAHQGERVAVGQRLMQAAADIF
                     LGWAEVPGSRDYYVRRLHDAKISIALETLGARALLEYGAACGWALARAHAKAGDAPAI
                     SGYLGTGDRFDRAVAAYAAAYADQVERDYQTFVQAIASGRLAAEGGDFAPVDFGP"
BASE COUNT          375 a          667 c          670 g          302 t
ORIGIN      
        1 cgccacgcgt ccagccctca ccgtcgtccg accccgcttc cgcgtcgccg gacgcgtgag
       61 cgccgccgcc agccccgaac ggatcgatct ctccggctac gaccctgcat ccaaagcggg
      121 gttttccgat aagaaggccg cgcgcaaggt gctgcgcgac gacatcgagc ggctggcgca
      181 cctcgacgac gtgtttgcgg cggcgcgcac ccacgcgatg ctgatcgtgc tccaaggcat
      241 ggacaccgcc ggcaaagacg ggatcatcaa acacgtgttg tcgggcgtca acccgcaagg
      301 cgtcaacgtg tacggctttc gtaagcccag cgaagaagag ctgctccacg attggctgtg
      361 gcgcgaatcc cgcgccctcc cggaacgcgg ccggatggcc atcttcaacc gttcctacta
      421 cgaagaagtg ctggtcgcgc gcgtgatgcc gggggttctc aagaaggaag ccgcaccgca
      481 cgacgctcgc cagtgggcgc atcgttacga agacatcaac gcgttcgagc ggcatctcac
      541 gcgcagcgga atggtagtcg taaagttctt cctccatata tcgcgcgacg agcagcgtaa
      601 acggttgtgc aagcggctcg agacgccgac caaaatgtgg aaagcctcgg attccgacct
      661 gcaggcgcac gagcgttggg atgcctacgc ccacgcctac gaggaaatgc tcggacatac
      721 gaacacggcg tgggcgccgt ggcacgtgat accggcggac cgcaagtggg tagcgcgcgc
      781 ggtggtgggg aacgtcctca tcgagacgct cgaagccctg catctgcact atcccgaacc
      841 gtcggcgcag cgccgcgaac tcttcgagcg gctcgcctcc cggctcgagg cggagggaga
      901 cggcggctcg ccggaaaagg cgaacgcgta ataggagagc gctcatgaag cttgtcgaga
      961 gcatcgatcc ggctacgctt gcggacgagc gtctcgttgc cgaatcgaac gagtttgcaa
     1021 tctacgatgc cggcaacgat tcgtacacgc tggtgcatcg ccacgcgggg gtgaactggc
     1081 aagggatcac gatctcgggc gacggcctct tccgggtcgc cgaactgctc gcgaccgcga
     1141 cgcgcgcgct ctaccgcgac atcgccggcg atctttcgcg gcggcgcagc cgctaagggc
     1201 cgaagtcgac cggagcgaag tcgccgccct cggcggcgag ccggcccgac gcaatcgcct
     1261 gcacgaacgt ttggtagtcg cgttcgactt gatccgcata ggccgcagcg tacgctgcga
     1321 ccgcgcgatc gaaccggtcg cccgtgccga gatatccgga aatggccggc gcgtcgccgg
     1381 ctttggcgtg ggcgcgggcc agcgcccatc cgcaggcggc gccgtactcg agcagcgcgc
     1441 gggcgccgag cgtttccagc gctatcgaaa tcttggcgtc gtgaagccgg cgcacgtagt
     1501 aatcgcggga tcccggaact tcggcccagc cgagaaagat gtcggcggcc gcctgcatca
     1561 ggcgctgccc gaccgcgacg cgctcgcctt gatgcgcgta gcggctgcgc gactcgggcg
     1621 gctcgagcac cgagcggcgc gcctccttta tctgcaagaa cagcgggtcg gcatcgtcgc
     1681 tgaggaagag cgaaacggtg cagcgggtgc cgacgctgcc gacgccgacg atcttcacgc
     1741 cgagatcttc gaaagtaaaa cggtcgaaca gctgccgccg gtcggcggag atcgactgcc
     1801 ggtagcgcgc gaagaacgtg tggatgagcc cggcccacgc gggcgcgcac tcgcgaaaat
     1861 gatagaccag cggcggcttg tcgacgatcc gcccgcgtcc gcgttcgacg attgcggcat
     1921 cgtgcaggct gccggccgcg gccgcgttct taccgaagaa cggccccagc tcgaccaggc
     1981 cgatctgcac gtaccagacc tcgagcgtcg aaag
//
LOCUS       DASUCJ010000105         1535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1376531, whole genome shotgun sequence.
ACCESSION   DASUCJ010000105 DASUCJ010000000
VERSION     DASUCJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376531"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFU90_01255"
     CDS             complement(<1..314)
                     /locus_tag="VFU90_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5478422.1"
                     /translation="MFTDIVGSTARWDANRAAMQEALQRHDAVVRAAIEGCGGRVFKT
                     VGDAFCAAFENVGAALDAAVAAQRNIDREDWRAVNGLRVRMAVHAGEAEERDDDYFGP
                     AV"
     gene            complement(401..1261)
                     /locus_tag="VFU90_01260"
     CDS             complement(401..1261)
                     /locus_tag="VFU90_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478423.1"
                     /translation="MLAIAILAAAVGFGANATPAPAATPPASPSPAAADPCGGPGRLL
                     ATADRPTVGFSSCAVAPDTAVFELGYQNQVTGTATAGSIESQAPQNFLRLGVATRFEL
                     DVIGPNYVRTRSFAAGIPDEVTSGVTDSGLGFKAELPPAGRWGIAFDGLYLPPNGSPF
                     LTAGNATFTGNLDAAYALSPSASIGTTIAVSSTGGYAADGTHARYGAMTPSLVITEQV
                     PHFYQFYAEYVFVSKLAPDEGGRAFTDFGIQKLLGSHLEIDLEYGHAFTAVPALKFDY
                     VGTGAVVQLP"
     gene            1281..>1535
                     /locus_tag="VFU90_01265"
     CDS             1281..>1535
                     /locus_tag="VFU90_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478424.1"
                     /translation="MRRADSVPQGRESAIRKPATMQKVNTDEIEELAWSSPKKKFSGA
                     GKQISEALGRKPLSTDINERHPFDVEILRVPPGTAPYPFHS"
BASE COUNT          267 a          517 c          498 g          253 t
ORIGIN      
        1 ttgacggccg gcccgaagta gtcgtcgtcg cgttcctcgg cctcgccggc gtgaaccgcc
       61 atccgtacgc gcaatccatt taccgcgcgc caatcctcac gatcgatatt cctttgcgcg
      121 gcgaccgcgg cgtcgagcgc ggcaccgacg ttctcgaacg ccgcgcaaaa cgcgtcgccg
      181 accgtcttga agacgcggcc gccgcagcct tcgatcgccg cgcgaacgac ggcgtcgtgc
      241 cgctggaggg cttcctgcat cgcggcgcgg ttcgcgtccc agcgcgccgt gcttccgacg
      301 atgtcggtaa acaaaaaagc ggtcgcccga ccgggggtct cgtgaacgtc ccacacgcag
      361 gtttgcttca gcgatcgccg ctccgagccc gctttgggtg tcagggcagc tgaacgacgg
      421 cacccgtgcc gacgtagtcg aacttcaagg ccggaacggc cgtgaatgcg tgtccgtact
      481 cgaggtcgat ttccaggtgg ctgccgagca gcttctgaat tccgaaatcc gtgaacgcgc
      541 gtccgccttc gtcgggcgcg agcttcgaga cgaacacgta ctcggcgtaa aattgataga
      601 agtgcgggac ttgctcggtg atcaccagcg acggcgtcat cgccccgtag cgcgcgtgcg
      661 ttccatcggc ggcgtagccg ccggtcgacg acaccgcgat ggtcgtcccg atgctggcgg
      721 acgggctcag cgcatacgcc gcgtcgaggt ttccggtgaa ggtcgcgttc ccggcggtga
      781 gaaacggcga gccgttgggc ggcaagtaca gcccgtcgaa cgcaatcccc cagcggccgg
      841 cgggtggaag ctcggctttg aatccgagtc cggagtcggt gacgccgctg gtcacctcgt
      901 ccggaatgcc cgcagcgaag ctgcgcgtgc gcacgtagtt cgggccgatc acgtcgagtt
      961 cgaagcgagt tgcaaccccc aggcgcagga agttctgcgg agcctggctt tcgatcgaac
     1021 ccgcggtcgc cgttccggtc acctggtttt gatagccgag ttcgaacacg gcggtgtccg
     1081 gcgcgaccgc gcacgagctg aaccccaccg tcggacggtc ggcggtcgcc agcagccggc
     1141 ccggaccgcc gcacggatcc gcggctgcgg gagacggcga agccggcggc gtggccgccg
     1201 gcgcgggcgt cgcgttcgct ccaaatccga cggccgccgc gaggatcgca atcgccagca
     1261 tgtcttgcct acgttacgat gtgcggcgcg ccgactccgt gccgcagggc cgggaatccg
     1321 caatccgcaa accggcgacc atgcaaaaag tcaacaccga tgagatcgaa gagctggcct
     1381 ggagttcgcc caagaagaaa ttttcgggag ccgggaagca aatatctgaa gcactcggcc
     1441 gcaaaccgct gtcgaccgat atcaacgagc gtcatccttt cgacgtcgag atcctccgcg
     1501 tacctcccgg caccgcaccg tacccgtttc attcc
//
LOCUS       DASUCJ010000106         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_933473, whole genome shotgun sequence.
ACCESSION   DASUCJ010000106 DASUCJ010000000
VERSION     DASUCJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933473"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFU90_01270"
     CDS             complement(<1..322)
                     /locus_tag="VFU90_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEU5478425.1"
                     /translation="MLDEVDAVVVDQVQCNELMRMHAKLGSRVPLHASGLGKALLAAL
                     PEDAVDRILHKRGLPRITAHTLDTPARLWDALAEIRRHGYTYDDGEHAVGLRCLAATI
                     HDEHG"
     gene            398..772
                     /locus_tag="VFU90_01275"
     CDS             398..772
                     /locus_tag="VFU90_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478426.1"
                     /translation="MREERTADDERLDADMPQAERLGPMHEAGGFERVEESMRRRRRQ
                     TDPLREVGERHAAIGVGKPFEECQAAREALALAGGTGIGASGRRARAFSGGRGARGDH
                     GLEVYVEVAGSIVSLPEADFKP"
     gene            933..>1176
                     /locus_tag="VFU90_01280"
     CDS             933..>1176
                     /locus_tag="VFU90_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5478427.1"
                     /translation="MSRMPAVEAAVRVMEREGIRHTFGVPGAAINPIYAAMKRRGSIE
                     HVLARHVEGASHMAEGFTRACAGNIGVCIGTSGPAGT"
BASE COUNT          219 a          353 c          410 g          194 t
ORIGIN      
        1 cgccgtgctc gtcgtggatc gtcgccgcga ggcagcgcaa accgaccgcg tgctcgccgt
       61 cgtcgtaggt gtagccgtgt cgacggattt ccgcgagcgc atcccacaac cgtgccggcg
      121 tatcgagcgt gtgcgcggtg atgcgcggca ggccgcgctt gtgcaggatg cgatcgacgg
      181 cgtcctcggg cagcgcggcg aggagcgcct tgccgagccc cgatgcgtgc aacgggaccc
      241 ggctgccgag cttcgcgtgc atgcgcatca gctcgttgca ctggacctgg tcgaccacca
      301 ccgcgtcgac ctcgtccagc accgcaagat tggcgctctc gcccgatagc tcgaccagac
      361 gacgaaggta aggatgcgtc cgggcgagga gatcgcgatg cgcgaggaac gcactgccga
      421 cgatgaacgc cttgacgccg acatgccaca ggccgagcgg ctcggtccga tgcacgaagc
      481 cggtggcttc gagcgtgttg aggagtcgat gcgtcgtcga cggcgacaga ccgacccgtt
      541 gcgcgaggtc ggtgagcgtc acgccgccat cggcgtcggc aagcctttcg aggagtgcca
      601 agccgcgcga gaggctctgg ctttggccgg cggcaccggc atcggtgcga gtggtcggcg
      661 ggcgcgggcg ttctcgggcg gtcggggcgc gaggggtgat catggattgg aagtgtacgt
      721 cgaagtcgcc ggatcgatcg tatcgttacc ggaagcagat ttcaagccat agtacatacg
      781 aaatgtaagc tattgattaa acatgatagt tgaagccata gtcgatgacc ttgcaaaatg
      841 gaaatggttt ctaaaattat tgacacgatc gccacaggcg actagactgc cgtgaattcg
      901 ccgcaatggt tcgcagtcga gaaggagtcg ccatgtccag gatgccggca gtcgaagcag
      961 cggtcagagt catggagcgc gaagggatcc gccacacctt cggcgtgccc ggtgcggcga
     1021 tcaatccgat ctacgccgcg atgaaacgcc gcggcagcat cgagcacgtg ctcgcgcgcc
     1081 acgtcgaggg cgcgtcgcac atggccgagg gcttcacgcg cgcgtgcgca ggaaacatcg
     1141 gcgtctgcat cggcacgtcg ggcccggcgg gcaccg
//
LOCUS       DASUCJ010000107         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_138837, whole genome shotgun sequence.
ACCESSION   DASUCJ010000107 DASUCJ010000000
VERSION     DASUCJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138837"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFU90_01285"
     CDS             complement(<1..222)
                     /locus_tag="VFU90_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478428.1"
                     /translation="MSLPAANGPPRLAASVILARPAAGGGFEAYMTRRSARSGFAPDA
                     FVFPGGVVDATDGGAAMRARTTGLEAAFAD"
     gene            250..684
                     /locus_tag="VFU90_01290"
     CDS             250..684
                     /locus_tag="VFU90_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU5478429.1"
                     /translation="MRIALGSDAAGDLPESIERWLRERGHEVTRFGALRHGDADGWPS
                     VARLVGDAVARGDADYGILCCWTGTGVSIAANKVAGVRAALCADAATAAGAREWNDAN
                     VLCLSLRATSVAVAEEMLAAWFSQSPTGEPAYRALIDELRTR"
     gene            797..1426
                     /locus_tag="VFU90_01295"
     CDS             797..1426
                     /locus_tag="VFU90_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478430.1"
                     /translation="MKSRWIAALLGAGLLLSAGPAMAAETWLPNVKDAPSRFSGPGVY
                     TGPPALAVTLSMVIAGGGPSNFQTLTLVKALAGDKTDAEVAALKKKFGDQKVTNFVTI
                     FPFVVSDSLKIAKAKGVALPAKPNPDPKDGEALAGALWAAGQTGHGFSVEVMLDRAVS
                     HPIHDQVMTDIDAKYGVAADADYHAVLNQAMHDLAAVYHFNAMGQPSSM"
BASE COUNT          280 a          527 c          554 g          236 t
ORIGIN      
        1 gtccgcgaac gccgcttcga gccccgtcgt gcgcgcgcgc atcgccgcac cgccgtcggt
       61 cgcgtcgacg acgccgcccg gaaagacgaa cgcatcgggc gcgaagccgc tgcgagcgga
      121 gcgccgggtc atgtacgctt cgaatccgcc gcccgcggcc gggcgcgcga ggatgacgga
      181 ggcggcgaga cgcggcgggc cgttggcggc aggtaggctc acgataccgg gaagcgtatc
      241 atgcaggcta tgcgaatcgc actcggcagc gacgcagccg gcgacctgcc cgaatcgatc
      301 gagcgctggc tccgggagcg cggtcacgag gtgacgcgct tcggggccct gcgccacggc
      361 gacgcggacg ggtggccctc ggtggcgcgg ctcgtgggcg acgcggtcgc gcgcggcgat
      421 gccgattacg gcatcctctg ctgttggacc ggtaccggcg tgagcatcgc cgccaacaaa
      481 gtggcggggg tgcgcgcggc gctctgcgcc gacgcggcca ccgcagccgg ggcacgcgaa
      541 tggaacgacg ccaacgtcct gtgtttgagc ttgcgcgcga cgtccgtcgc cgtcgccgaa
      601 gagatgctgg cggcttggtt ttcacagtcg cccaccggcg aaccggccta ccgcgccctg
      661 atcgacgagc tgcgcacgcg ctaggtttcc ggatggccgc gcggcggcgg cgccaatcca
      721 aacgccgtct cactccgtta tccaagcact ccacgaagca agggacttga gaatgaaaga
      781 gaggcttgga ataattatga aatctcgctg gatcgcggcg ctgctcggcg ccggattgct
      841 gctctcggcc gggccggcga tggccgccga gacgtggctg ccgaacgtga aggatgcccc
      901 gtcgcggttc tcgggaccgg gcgtctacac cgggcccccg gcgctcgcgg tgacgctctc
      961 gatggtcatc gccggcggcg gcccgagcaa cttccaaacg ctgacgctgg tgaaggcgct
     1021 ggccggcgac aagaccgacg ccgaggtagc ggcgctcaag aagaagttcg gggaccagaa
     1081 agtaacgaat ttcgtgacga tcttcccgtt cgtcgtgagc gattcgctga agattgccaa
     1141 ggccaaaggc gtcgcactcc cggctaagcc gaatccggat ccgaaggacg gcgaagcgct
     1201 ggccggcgcg ctctgggcgg ccggtcaaac ggggcacggt ttcagcgtgg aggtcatgct
     1261 cgaccgggcg gtgtcgcatc cgattcacga tcaagtgatg accgacatcg atgcaaagta
     1321 cggcgtcgcc gccgacgcgg actaccacgc cgtgctcaat caggcgatgc acgatctggc
     1381 cgcggtgtac catttcaacg cgatgggcca gccgtcgtcg atgtagacgg cgcacccccg
     1441 agcgtaaacg agcaggaggg tggcgcaatc gcgccaccct ctttctgcct taccgagccg
     1501 gccgtagaac tgacgctgcg tccggctact ccacccccga cgaatacggc gaaaagcgct
     1561 tcaccgtata ttcagcgccc ggagaagccg attctac
//
LOCUS       DASUCJ010000108         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_872284, whole genome shotgun sequence.
ACCESSION   DASUCJ010000108 DASUCJ010000000
VERSION     DASUCJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872284"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1022
                     /locus_tag="VFU90_01300"
     CDS             90..1022
                     /locus_tag="VFU90_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5478431.1"
                     /translation="MGAVVFWIALVTLPLWLTHVGGYTALGTKILVYGIATLALNLLL
                     GFTGGLSFGHAAWFGLGAYGAGMTLKYLAPSTPLAILVGTVVGGVAATVLGPLVMRRR
                     GIYFAMITIAIGQLFYFVAVRWNNVTGGEDGLAGFERAPLHFGKWVYDIHDDTKYYYL
                     VLVCFAIAVVVMKLLLASPLGHTWVAIRENRRRLQFLGVRIDLYVWAAFAISGTLTAL
                     AGTLNAMLFNFTSPQDLHWILSGNFVIMIVLGGMRKFWGPLLGVAIFVVAQDYLSSVT
                     DNWMTAIGTLFVLIVLLFPKGLLGMLGRRGKEAA"
     gene            1019..>1328
                     /locus_tag="VFU90_01305"
     CDS             1019..>1328
                     /locus_tag="VFU90_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5478432.1"
                     /translation="MSAGDARALLRLSGVCRHFGRLQAVQDVSFAMNEGELRAVIGPN
                     GAGKTTFFNLITGFVPPTSGDIWFGDENIRDESVVRRVRRGIVRTFQITEIFPDLTVY
                     E"
BASE COUNT          183 a          426 c          446 g          273 t
ORIGIN      
        1 atgctgatcg tgctcgtgct gcgaccgcgt ggcctgctcg gccaggaagg gctcttcgaa
       61 tgatcgcgtc gcggcgaaag ctgcgcctcg tgggagccgt ggtgttctgg atcgcgctcg
      121 tcacgctgcc gctgtggctc acccatgtcg gcgggtacac ggcactcggc acgaagatcc
      181 tcgtctacgg catcgcgacg ctggcgctca acctgcttct cggcttcacc ggcgggctgt
      241 cgttcgggca cgccgcgtgg ttcggcctcg gcgcgtacgg cgcgggcatg acgctcaagt
      301 acctcgctcc gtcgacgccg ctcgcgatcc tcgtcgggac cgtcgtcggc ggagtcgcgg
      361 caaccgtgct cggtccgctc gtgatgcgcc ggcgcggcat ctacttcgcg atgatcacga
      421 tcgcgatcgg gcaactcttc tattttgtcg cggtgcgctg gaacaacgtc accggcggcg
      481 aggacgggct cgccggcttc gagcgcgcgc cgcttcattt cgggaagtgg gtctacgaca
      541 tccacgacga taccaagtac tactacctgg tgctcgtgtg cttcgcgatc gccgtcgtcg
      601 tcatgaagtt gctcctcgcc tcgccgctcg gccatacctg ggtcgcgatc cgcgagaacc
      661 gtcggcgcct gcagttcctc ggcgtgcgca tcgacctcta cgtgtgggcg gcgtttgcga
      721 tctcggggac gctcactgcg ctcgccggaa cgcttaacgc gatgctgttc aacttcacgt
      781 cgccgcagga cctgcactgg atcctgtcgg ggaacttcgt gatcatgatc gtgctgggcg
      841 gcatgcggaa gttctggggt ccgctcctcg gcgtcgccat cttcgtcgtg gcgcaggatt
      901 acctgtcgag cgtgaccgac aactggatga ccgcgatcgg gacgctgttc gtgctgatcg
      961 tgctgctgtt cccgaagggg ctgctcggca tgctcgggcg gcgcggcaag gaggcggcgt
     1021 gagcgccggc gatgctcgcg cgctgctgcg cctttcgggc gtgtgccgtc atttcggacg
     1081 gctgcaggcg gtgcaggacg tatcgtttgc gatgaacgaa ggcgagctgc gcgccgtcat
     1141 cggcccgaac ggcgccggca agacgacgtt cttcaatctg atcaccgggt tcgttccgcc
     1201 gacttcggga gacatctggt tcggcgacga gaacattcgt gacgagtcgg tggtacggcg
     1261 tgtccgccgc ggcatcgtgc ggacgttcca gatcaccgag atcttccccg atctcaccgt
     1321 ctacgaga
//
LOCUS       DASUCJ010000109         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1071092, whole genome shotgun sequence.
ACCESSION   DASUCJ010000109 DASUCJ010000000
VERSION     DASUCJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071092"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1127)
                     /gene="fmt"
                     /locus_tag="VFU90_01310"
     CDS             complement(159..1127)
                     /gene="fmt"
                     /locus_tag="VFU90_01310"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5478433.1"
                     /translation="MSTANSTSIKPVTSGRRSPRKRKKLPKPARRCERKPINGPALTS
                     LFFGTSAFAVPSLHAAATHSRVAGVVTQPDRPAGRGQRSMPSPVKSAALELGLCVHEP
                     ERLKAFARELQDGSFDLFALAAYGRILPPELLALPRLGALNVHPSLLPKYRGATPIQT
                     ALRNGDRETGVTVMLMDAGMDTGAIVLQRRVAIESGETYGELHDRLAAAGADLLAEAF
                     DLAAEGDLPRSPQHGEPSVTRPTEKADLIVDWSWPARRIVDHVRAYSPQPAARAVVDG
                     EVVKLLRAHVAGDGTLAIDQLVAPNRGPASGEAYLRRRKLSQFPRR"
     gene            complement(1015..1548)
                     /gene="def"
                     /locus_tag="VFU90_01315"
     CDS             complement(1015..1548)
                     /gene="def"
                     /locus_tag="VFU90_01315"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5478434.1"
                     /translation="MPYLREIVLDGHPALRKVAKKVDPKEIGAPLFQQLIDDMFETMY
                     AAPGVGLAAPQVNVSKRLFVIDVHDDEHPPAVVINPKIESAEEEVELTEGCLSVPGMV
                     GEITRFKRIAVSGLDRDGNKIRLEGEGLFAQCLQHEIDHLNGELYLDKARNVRPAVTE
                     EEEEAPEAGEAVRAQAY"
     gene            1676..2856
                     /locus_tag="VFU90_01320"
     CDS             join(1676..1964,1964..2856)
                     /locus_tag="VFU90_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5478435.1"
                     /translation="MKGKSSKRERYSAEFKLEAVRLMQTGTKDVTQLARELDVPRQYL
                     HAWFRQAQRRQGQAAGDIFPGNGNLSSVDAELDRLRKENAQLKEDNEILKKANGLLRQ
                     KHPLRYAFIDVQRRWHSIIALCRNLEVSKAGYYAWRERKPSARDQTDEHVGEVIRSIH
                     DQSRRRYGAPCVHAELRDRGIRIGRKRVARLMKSHGLRAKKARPRRVVTTDSKHGYPV
                     APNVLNRQFDQPMANRAWVCDITYFSTKEGWLYLAVVMDLFSRRIVGWSMSAFIDAEL
                     VLNALRMAIEQRRPQRGLLVHSDRGSQYACRAYRNVIAQHGFVASMSRKRDCWDNAVV
                     ESFFASIKSDLKGEPVWATRTAARSAIFDYIETWYNRQRRHSTLGYVSPIAFERRHGV
                     S"
     gene            complement(3014..>3485)
                     /locus_tag="VFU90_01325"
     CDS             complement(3014..>3485)
                     /locus_tag="VFU90_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478436.1"
                     /translation="AQCFTNPNGTTTVYYGGWASPYTDSYSSDAVFTTQISQGMVDRR
                     SPGFSWRASATFTSNDKRVTFIAGDAWFNYGNPLAAQITNEWTLDGIYRFSPVSSGPY
                     RGFFVRYRYAQRSQSNTFCGASATSCPPGTPAGTTFLGGLPLFKYNRAQLEYDF"
BASE COUNT          626 a         1124 c         1126 g          609 t
ORIGIN      
        1 gccgtaggcc aactcggtcg cgaaggcgcg gtcgcgcgga ccggcttgcg ccttgcgggc
       61 gcgatagtcg agcgcttcct gcgcgccgcg ttccggcacc ggcgaccccg ccggcgggaa
      121 aatgtcgcgc acgaccgcca gcgccaactc ccgcgcgttc atcggcgcgg gaattgcgaa
      181 agcttacgcc gccgcaggta ggcctcgccg ctggccggtc cgcgattggg ggcgacgagc
      241 tgatcgatcg cgagcgtacc gtcgccggcg acgtgcgcgc gcagcagttt gacgacctca
      301 ccgtcgacga ccgcccgcgc ggcgggctgg ggagaatacg cgcgcacgtg gtcgacgatc
      361 cggcgcgcgg gccacgacca gtcgacgatc aaatcggctt tttcggtcgg ccgcgttacg
      421 ctcggctcgc cgtgctgcgg cgaccgcggc agatcgcctt cggcggcgag gtcgaacgct
      481 tcggcgagca gatctgcgcc ggcagcggcg aggcgatcgt gcagctcccc gtacgtctca
      541 ccggattcga tggcgaccct gcgctgcagc acgatcgcgc cggtatccat gccggcgtcc
      601 atcagcatca cggttacgcc cgtttcgcgg tcgccgttgc gaagcgcggt ttgtatcggc
      661 gtcgcgccgc ggtacttcgg cagaagcgac gggtgcacgt tgagcgcgcc caagcgcgga
      721 agcgcgagca gctcgggcgg gaggatgcgt ccgtacgcag ccagggcgaa taggtcgaag
      781 ctgccgtctt ggagttcccg tgcgaacgct ttgaggcgtt ccggttcgtg aacgcacaac
      841 cccagttcga gagccgcgga cttgaccggc gacggcatcg agcgctggcc gcgaccggcg
      901 ggccgatcgg gctgcgtcac gacgcccgcc acccgcgaat gcgtcgcggc ggcgtgcagg
      961 ctgggaaccg caaatgcact cgttccgaag aagagcgacg taagggctgg gccgttaata
     1021 ggcttgcgct cgcaccgcct cgccggcttc gggagcttct tcctcttcct cggtgaccgc
     1081 cggccggacg ttacgggctt tatcgaggta gagttcgccg ttgagatggt cgatctcgtg
     1141 ctgcaggcac tgggcgaaca gcccttcgcc ctcgagacgg atcttgttgc cgtcgcggtc
     1201 gagcccgctg acggcgatgc gtttaaagcg cgtgatctcg ccgaccatgc ccggtaccga
     1261 caagcagcct tcggtgagct ccacctcttc ttcggccgat tcgattttag ggttgatgac
     1321 gacggccggc ggatgctcgt cgtcgtgcac gtcgatcacg aacaagcgct tcgagacgtt
     1381 cacttgcggc gcggcgagcc cgacgccggg cgccgcgtac atcgtctcga acatgtcgtc
     1441 gatcagctgc tgaaacagcg gggcgccgat ctctttgggg tcgactttct ttgcgacctt
     1501 tcgcaacgcc gggtggccgt cgaggacgat ctcgcgcagg tagggcatgg cgcgactatt
     1561 ccaggaaagt ccagccgtgt catcctgcgc gcagcgcctc aacggtgtca tcctgggcct
     1621 gacccgattt tgtggactcc aagataagcg ccacacggcg cggaagggga gtctcatgaa
     1681 agggaaatcg agcaagcgcg aacggtactc ggccgagttc aagctcgagg ccgttcgcct
     1741 gatgcaaacg gggaccaagg atgtgacgca gttggcacgc gagctggacg ttccgcgcca
     1801 atatttgcac gcttggtttc gccaggcgca gcggcgtcag ggccaagcag ccggcgatat
     1861 cttcccaggt aacggcaatc tctcgtcggt cgacgccgaa ctcgatcggc tgcgcaaaga
     1921 gaacgcccag ctcaaggaag acaacgagat cctaaaaaaa gcgacggcct tcttcgccaa
     1981 aaacacccgc tgagatatgc gttcatcgac gtccaacggc gatggcattc gatcatcgcg
     2041 ttgtgccgca atctcgaggt gtcgaaggcc ggctactatg cgtggcggga acgcaagccc
     2101 agcgctcgcg accaaaccga cgagcacgtc ggcgaggtca tccgttcgat tcacgaccag
     2161 agccgccggc gctacggagc tccctgcgtg cacgccgagc tgcgcgaccg cggtattcgg
     2221 atcgggcgca aacgcgtggc tcggctgatg aaatcgcacg gtttacgggc gaagaaagcc
     2281 cggcctcgcc gggtggtcac aaccgactcc aagcacggct acccggttgc tcctaacgtc
     2341 ctcaatcgcc agtttgacca gccgatggcc aatcgggctt gggtctgcga tatcacgtat
     2401 ttctcgacca aagagggatg gctgtacttg gcggtcgtga tggacctgtt ttcccggcgg
     2461 atcgtcggtt ggtcaatgag cgcgttcatc gacgccgagc tggtgctcaa tgcgctgcgc
     2521 atggccatcg aacagcgtcg gccgcagcgc ggcctgctcg tccatagcga tcgcggcagc
     2581 cagtacgcct gccgagcata ccgtaatgtc atcgcccaac acggcttcgt tgcgagcatg
     2641 agtcgcaagc gcgactgctg ggataatgcg gtcgtcgaaa gcttctttgc ctcaatcaag
     2701 tccgacctca aaggcgaacc cgtctgggct acgcgcaccg ccgcccggtc agctatcttc
     2761 gactacatcg aaacctggta taatcgccaa cgccgacatt caaccctcgg ctacgtcagc
     2821 ccaatcgcct tcgaacgccg gcacggtgtc agctaactac ctgtccacaa aatcgggcca
     2881 ccctcaggat gacacaccgc gcgcccttcg actacggcgc tgcgcgcctc cgctcaggat
     2941 gacacgccga gcgcctccgc tcaggatgac acaccgagcg cctccgttca ggtaacacga
     3001 ggtcggcgtt ttgctagaag tcgtattcga gctgggcgcg attgtacttg aacaacggca
     3061 gcccgcccag gaaggtcgtt cccgccggag ttccgggagg gcagctcgtc gcactcgcgc
     3121 cgcagaacgt gttgctctgc gagcgctggg cgtagcggta gcgcacgaag aacccacggt
     3181 acgggccgct cgagaccggg ctgaaacggt agattccgtc gagcgtccac tcgttggtga
     3241 tctgcgcggc caagggattc ccgtagttga accacgcgtc gccggcgatg aacgtcaccc
     3301 gcttgtcgtt cgaggtaaac gtcgccgaag cgcgccacga gaagcccggc gaccggcggt
     3361 ccaccatgcc ttgcgaaatc tgcgtcgtga aaacggcgtc gctggaataa ctgtcggtgt
     3421 acgggctcgc ccagccgccg tagtacacgg tcgtcgtgcc gttgggattg gtaaagcact
     3481 gggcc
//
LOCUS       DASUCJ010000110         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_933514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000110 DASUCJ010000000
VERSION     DASUCJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFU90_01330"
     CDS             <1..608
                     /locus_tag="VFU90_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5478437.1"
                     /translation="GKGGHGAYPHTAVDAIPPAAALILALQNVAARETDPLESVVLTI
                     GTIRGGYRNNVIADEVEMSGTLRAFDPAIRDALEARIRRIADGIAAAYGATAGVRVVR
                     GYPPVENDARLAEAFARYLREHSRIRVERMMPTMGGEDFAYFAQRIAGLQVRLGIRNE
                     RAGAVHPGHSPEFRIDENALAVGVETLAAFARAAGSGAIGG"
     gene            763..1173
                     /locus_tag="VFU90_01335"
     CDS             763..1173
                     /locus_tag="VFU90_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478438.1"
                     /translation="MLCAVEKWPVWLSFVRSARSSAAGDSIGVGSDVVVRTSLPGGEE
                     QHYEVDAFTPNYRLSLVGAYSVRRRIDFRIERRTSRARIHVRLSYPAYHGRLGELLDS
                     WRHRRKLTAALADALVHFKGLVEYRRNDPILADL"
     gene            1248..1547
                     /locus_tag="VFU90_01340"
     CDS             1248..1547
                     /locus_tag="VFU90_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU5478439.1"
                     /translation="MPLYDYKCTRCGAVSEVRHGFDQTHDEPCPACGGSLARVFNPAP
                     IVFKGSGFYVTDSRKSAGKSKADAPAAAAKTEAPAADATPAAAPKKDAKPSDSAA"
     gene            1551..1877
                     /locus_tag="VFU90_01345"
     CDS             1551..1877
                     /locus_tag="VFU90_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478440.1"
                     /translation="MLWIVPAAGAAFALIAVVVGAVAVARANRELQPVLGRLRAGAAI
                     ADPQALQRSLGRLNEDVAQGKALVERAASAVAEIRAGLSALRLREAIVALRLAGLAVR
                     ALISLR"
     gene            complement(1874..3151)
                     /gene="aroA"
                     /locus_tag="VFU90_01350"
     CDS             complement(1874..3151)
                     /gene="aroA"
                     /locus_tag="VFU90_01350"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5478441.1"
                     /translation="MLGRLTFSPGRLRGELSVPGDKSISHRALIAAASLRHPVRITNL
                     NPGRDVTATFEGLRALGVRAERGPADAVVQGAPLRPPGTLQCHNSGSTARMLLGACAG
                     AGVAARFDGDDSLRRRPMEPVAAQLRAFGARIDTAGGYLPLDLHGTPAIQTRSFILLA
                     PSAQVKSALLFAGLFANAPVRVAGDRGSRDHTERLLRYLGGTVAWDGRTVTLAAEPLV
                     PRDIQVPGDFSSAAFFLTAAAIVPGSSIVVRDTGVNPTRTGLLEVLTRMGARLELRNS
                     RTVCDEPRADVAVEYAPLRGIAVDRDLALRAVDEIPLVALAAAFAEGETRIAGVGDLR
                     FKESDRVAGIVRLLEAVGVAVDRTGNDLKIRGGNATRASGAGIDARGDHRIAMAAAVL
                     GCAAGPISVEGDPAVGVSFPGFVETLDAVRLPA"
BASE COUNT          572 a         1115 c         1191 g          538 t
ORIGIN      
        1 gcgggaaagg cggccacggc gcgtacccgc acaccgccgt cgacgcaatc ccgccggcgg
       61 cggcgttgat actcgcactg cagaacgtgg ccgcacgcga gacggatccg ctcgaaagcg
      121 tggtgctcac gatcgggacg atccgcggcg gctaccggaa caacgtgatc gccgacgagg
      181 tcgagatgtc gggaacgctg cgcgcgttcg atccggccat ccgcgacgcg ctggaagccc
      241 ggatccgccg gattgccgac ggcatcgccg ccgcgtacgg cgccaccgcc ggagtacgcg
      301 tcgtccgcgg ctatccgccg gtcgaaaacg acgcgcggct tgccgaggcg ttcgcacgtt
      361 atctccgcga gcatagccgg attcgcgtag agcggatgat gccgaccatg ggaggcgagg
      421 atttcgcgta tttcgcgcag cggatcgcgg ggctgcaggt gcgcctcggg atccgcaacg
      481 agcgcgccgg ggccgtccac ccgggccaca gcccggagtt tcgaatcgac gaaaacgcgc
      541 ttgcggtggg cgtcgaaacg ctggcggcgt ttgcgcgcgc ggccggcagc ggtgcaatcg
      601 gtggatagcc gtccacaatt tgttgtggac gattaagcga aaggtacgat atatcgcacg
      661 ccgaaggggt gcgcgatggc tgtaaccgac gttcccgctc aaccggggcg gtggttgagt
      721 gcaagcctcg acgtcgatag cacggcggaa gaggctttcg agttgctgtg cgcggtcgag
      781 aagtggccgg tctggctgtc gttcgtacgc agcgcgcgct ccagcgcggc cggcgattcg
      841 attggagtcg ggagcgacgt cgtcgtccgc acctccctgc ccggcggcga agaacagcac
      901 tacgaggtcg acgccttcac ccccaactac cggttgtcgc tggtgggcgc gtactcggtg
      961 cggcggcgga tcgactttcg catcgaacgc cgcacctcgc gcgcgcgcat ccacgtgcgg
     1021 ctgtcgtatc cggcgtacca cgggcggctc ggcgaactgc tggacagctg gcggcatcgc
     1081 cgaaagctga ccgcggcgct ggcggacgcg ctcgttcatt tcaagggact ggtcgagtac
     1141 cgccgcaacg atcccatctt ggccgatctt taggcaagca tttccgaacc tcgcgccctt
     1201 tggcagtcgt tgaccaggac tgctagatgc gggtatactg ctcgggaatg ccgctctatg
     1261 actacaaatg cacgcgctgc ggagccgttt ccgaggttcg gcatgggttc gatcaaacgc
     1321 acgacgagcc gtgtccggcc tgcggcggca gcctcgctcg ggtcttcaac cccgcgccga
     1381 tcgtgttcaa aggatccggg ttttacgtaa ccgactcacg caagagcgcc ggcaagtcca
     1441 aggccgacgc cccggcggcc gccgcaaaga cggaggcccc ggctgcggat gcgacgccgg
     1501 ctgccgcgcc taagaaagac gccaagccga gcgattcggc ggcctgaacc gtgctctgga
     1561 tcgttccggc ggcgggtgcg gcgttcgcgc tgattgccgt cgtcgtgggt gccgttgccg
     1621 tcgcccgtgc gaaccgcgag ctgcaacccg tgctcggtcg cctgcgagcc ggcgcggcaa
     1681 ttgccgatcc gcaagcgctg cagcgttcgt tgggtcgact gaacgaggac gtcgcccaag
     1741 ggaaagcttt ggtcgaacgc gcggcgtcgg cggtcgccga gatccgagcg ggcttgagcg
     1801 cgttgcggct gcgcgaggca atcgttgcgt tgcggttggc cggcctcgcc gtccgcgccc
     1861 tgatttcgct ccgctaagcc ggtaaacgaa ccgcgtcgag cgtttcgacg aacccgggga
     1921 agctcacgcc gaccgccgga tcgccctcga ccgagatcgg gccggccgcg catcccagaa
     1981 cggcggccgc catcgcgatc cggtggtcgc cgcgggcgtc gatgccggcc ccggaagccc
     2041 gggtcgcgtt tccaccacga attttgaggt cgtttccggt gcgatcgacg gccacaccca
     2101 cggcttcgag cagtcgcacg attccggcga cgcgatccga ctccttaaag cgcaggtccc
     2161 ccacccccgc aatccgggtt tcgccctcgg caaaggcggc cgcgagggcg accagcggga
     2221 tctcgtcgac ggcgcgcagc gccaaatcgc gatcgacggc gatgccgcgt aacggggcgt
     2281 actcgacggc gacgtccgcc cgcggctcgt cgcacaccgt gcgcgagttg cgcagctcga
     2341 ggcgcgcgcc catccgcgtc agcacctcga ggagcccggt ccgggtcgga ttgacgccgg
     2401 tatcgcgcac gacgatcgaa ctgccgggca cgattgccgc cgcggtgaga aaaaacgccg
     2461 ccgaggaaaa gtcgccggga acttggatgt cgcggggaac cagcggttcg gccgcgagcg
     2521 ttaccgtgcg gccatcccat gcgacggttc cgcccagata acgaagaaga cgctcggtgt
     2581 gatcgcgcga gccgcggtcg ccggcaaccc gtaccggcgc gttcgcgaac agccccgcga
     2641 acagcagtgc cgattttact tgggccgagg gcgcgagcag gatgaagctt cgcgtctgaa
     2701 tcgccggcgt gccgtgcaga tcgagcggca gatagccgcc ggcggtgtcg atccgggcgc
     2761 cgaaggcccg caactgcgcg gcgaccggtt ccatcgggcg ccgccgcagg gagtcgtcgc
     2821 cgtcgaatcg tgcggcgacg ccggcgccgg cgcacgcgcc gagcagcatc cgtgccgtcg
     2881 atcccgagtt gtgacactgc agcgttcccg gcggccgcag cggggcaccc tggacgacgg
     2941 cgtccgccgg cccgcgttcg gcgcgcacgc cgagcgcgcg caatccttcg aacgtggcgg
     3001 taacgtcccg accgggattc aggttggtaa ttcgaacggg atgccgcagc gacgcggctg
     3061 cgatcaaggc gcggtgcgag atcgatttgt cgccgggcac ggaaagttcg ccgcgcaacc
     3121 gtcccgggga aaacgtgagc ctgccgagca tggggctctt tgagttcacg gatagagcgg
     3181 tgcgttcccc ggatgcggcg cgttgcgcgt atcgtcggcg accgcgcacg ccgaagtttt
     3241 tgcttaaaag ggcgaaaaaa gtccgaaatt cccgtaggaa attggggatt gaagcggtag
     3301 tcactcgccc aagtgcttta ctttcacacg gaaaggtacg gatgaacaag gtagatttga
     3361 ccaggtcggc gatggaacgg ctcggccttt ctcggaagga tgccgttgag ctcgtc
//
LOCUS       DASUCJ010000111         1230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_811081, whole genome shotgun sequence.
ACCESSION   DASUCJ010000111 DASUCJ010000000
VERSION     DASUCJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1085
                     /gene="lipB"
                     /locus_tag="VFU90_01355"
     CDS             405..1085
                     /gene="lipB"
                     /locus_tag="VFU90_01355"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5478442.1"
                     /translation="MGTRCTLLRPGIVDYQRACGFQRRIADAVRDGSDPALILLEHSP
                     VYTLGVRGHGGEHLLSSRERLEALGAQVVESDRGGDVTFHGPGQLVGYPILDLRRFGE
                     GPVWYVRGLESVLIDTLARFGIVAGRVAGRPGVWVGDAKIAAIGVRVSRGVTTHGFAL
                     NVNTDLSYFSHIVPCGLPDAGVTSMRELMGETPTMREVEDELVDAFSWQFGMDLFEEP
                     SMEAAGGR"
     gene            1075..>1230
                     /locus_tag="VFU90_01360"
     CDS             1075..>1230
                     /locus_tag="VFU90_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5478443.1"
                     /translation="MVAEARPERKPEWLKVRFPGGERYQRLQRLMRDAGLHTVCEEAH
                     CPNVGECW"
BASE COUNT          199 a          401 c          424 g          206 t
ORIGIN      
        1 ctgccgtgaa gacgacgccc gccgggacgc aggtgcgcgt gccatgtgag gccacgcacg
       61 atgggagcgg ccagagccac gaccacacgc ccgaagcggg tacgacgccc gaagcgaacc
      121 acgattcatc tcactgacgt ccacgacggg ggagcatacc gaggcccggg caatcgcccg
      181 ggcctcattg cgtcccggcg ctatgtcaga tgcgtgagga cttgccgctc gcgacatcgc
      241 gggccgtcgc gctgcattcc atgatttccg cgcggcaaag gaccgattct tctgttgcgg
      301 agcggtgaca tgcgaacgca aggccgatac tccgtcaggt tcagatgagc gtgctatgct
      361 ccaagaagcg ccgatgcggc gcgcgaaccg gaaagaacgg cgctttggga acccgctgta
      421 ccctgctccg acccggtatc gtcgattacc agcgcgcctg tgggtttcag cgccggatcg
      481 ccgacgctgt tcgcgacggc tctgatcctg ccctcatcct gctcgagcat tcgcctgtct
      541 acacgctggg tgtccgcggc cacggcggcg agcatctgct gtcgagccgc gagcgcctcg
      601 aggcgctcgg cgcgcaggtc gtcgaatccg accgcggcgg cgacgtcacg tttcacggcc
      661 cagggcaact cgtcggctac ccaatcctcg acttgcgccg gtttggtgaa gggccggtgt
      721 ggtacgtccg cggcctcgaa tcggtcctca tcgacacact cgcgcgattc ggcatcgtcg
      781 ccgggcgtgt cgccggtcgg cccggcgtat gggtggggga cgcgaagatc gccgcgatcg
      841 gcgtgcgggt ctcgcgtggc gtcacgacgc atggcttcgc gctcaacgtg aatacggacc
      901 tctcgtactt ctcgcacatc gtgccgtgcg gactgcccga cgccggcgtg acgtcgatgc
      961 gcgagttgat gggcgagact ccgaccatgc gggaggtcga ggacgaactc gtcgacgcgt
     1021 tctcgtggca gttcgggatg gacctgttcg aagagccgag catggaggcc gctggtggtc
     1081 gctgaggcgc gtccagaacg caagccagaa tggctgaagg ttcgcttccc cggcggcgag
     1141 cgctaccagc ggctgcagcg gctgatgcgc gacgctggcc tgcacacggt ctgcgaggaa
     1201 gcgcactgcc cgaacgtcgg cgagtgctgg
//
LOCUS       DASUCJ010000112         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1468406, whole genome shotgun sequence.
ACCESSION   DASUCJ010000112 DASUCJ010000000
VERSION     DASUCJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468406"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..552)
                     /locus_tag="VFU90_01365"
     CDS             complement(4..552)
                     /locus_tag="VFU90_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5478444.1"
                     /translation="MMQRAVLGMVLLFALGDSCTPSPNVVGVQDYGQVTGRVLDAMTN
                     RPIANALVSVGSLYTASADARGAFVLGRVPAGDQTVTGRAPGYSTGSADVTVLKDKTV
                     SAGYVRLVPLIHPEGQPTLPPPATPTPQPSPTPGGSPSAAPTQEPAPGIAPTPGGTPA
                     IQPTPTPTAAAVPITSPTPTAS"
     gene            645..998
                     /locus_tag="VFU90_01370"
     CDS             645..998
                     /locus_tag="VFU90_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5478445.1"
                     /translation="MNPYEPVAIYLCVALAAALMFSLLPGLIQRRKPNPQKLEAYECG
                     VEPTSAVAGRFPVRFYLVAMLFVIFDVEAASFYPWAVQMHALRVFGLLEMIVFIIVLA
                     IGYAYVWKKGGFVWR"
     gene            989..1474
                     /locus_tag="VFU90_01375"
     CDS             989..1474
                     /locus_tag="VFU90_01375"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5478446.1"
                     /translation="MAVGEGPGHFMLAKLDDVARWAQSSSVWPLTMGLACCAIEMMTV
                     TSSEYDIARLGSEVFRSSPRQADLMIVSGRVAWKMAPVVKRLYDQMADPKWVISMGAC
                     ASSGGIFDNYAIVQGIDTIVPVDVYVPGCPPTPDGLLYAVNLIQQQIREGGRGGILAR
                     S"
     gene            1485..>2082
                     /locus_tag="VFU90_01380"
     CDS             1485..>2082
                     /locus_tag="VFU90_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5478447.1"
                     /translation="MPSTQTPPIAGAAIDPPALRPPIDDAQIERVAPSALRARLEALR
                     AAGFTMLLDLGAVDYLQRDPRFDVIYHLLKLPPAAAGVAEVGSPARVRILCGVGGDDP
                     RLPTAMDLWKSADWAEREIFVLFGITFDGHANLRRIQMPYDWEGHPLRRDYPVRGPAR
                     ERAPRPAFALKSNVSAGTPPSGTTLEALQEQVKRVREGD"
BASE COUNT          321 a          663 c          737 g          361 t
ORIGIN      
        1 cgcttaggat gccgtcgggg ttggcgaggt tatggggacg gcggcggcgg tcggcgtcgg
       61 ggttggttgg atggcgggcg tgccgccggg cgtcggggcg atgccggggg cgggctcttg
      121 ggtgggcgcc gccgacggcg agccgcccgg ggtcggtgac ggttgcggcg tcggcgtggc
      181 gggcggcgga agcgtgggct gcccctcggg atggatcagc ggaacgagcc ggacgtagcc
      241 ggcggagacc gtcttgtctt tgagtacggt cacatcggcg ctgccggtcg agtacccggg
      301 tgcgcgtccg gtgaccgttt ggtcgccggc cggaaccctg ccgagcacga aagcgccgcg
      361 ggcatcggcc gaggcggtgt agagcgagcc gaccgaaacg agcgcgttgg cgatcgggcg
      421 attcgtcatc gcgtcgagca cccgcccggt cacttgcccg taatcctgaa cgccgacgac
      481 gttgggcgac ggcgtgcacg aatcacccaa ggcaaaaagg agaaccatcc caagcaccgc
      541 tcgctgcatc atgccgtctc tttacgcgcc gcccggccga agtccccagt cgcgctcacg
      601 cttgccaggt tcccggcacg tgcttgtgaa aactttcaca agccatgaat ccgtacgaac
      661 cggtcgcgat ttacctctgc gtcgcgctgg ccgcggcgct gatgttcagc ctgctgccgg
      721 ggctcatcca gcgacgcaag cccaatccgc aaaagctcga agcctacgag tgcggcgtcg
      781 aaccgacgtc ggcggttgcc ggccggttcc cggtgcggtt ctatctcgtc gcgatgctgt
      841 tcgtgatctt cgacgtcgaa gcggcgtcgt tttacccgtg ggcggtgcaa atgcacgcgc
      901 tgcgggtgtt cggactgctc gagatgatcg tcttcatcat cgtgctcgcc atcggctacg
      961 cgtacgtgtg gaagaagggc ggcttcgtgt ggcggtaggc gagggaccgg gccacttcat
     1021 gctggccaag ctcgacgacg tcgcccgctg ggcgcaaagc tcgtcggtct ggccgctgac
     1081 gatggggctc gcgtgctgcg cgatcgagat gatgacggtc acgtcgtcgg agtacgacat
     1141 cgcgcggctg ggatccgaag tgtttcgcag ctcgccgcgg caagccgacc tgatgatcgt
     1201 ctcggggcgg gtcgcctgga agatggcgcc ggtggtgaag cgtctctacg atcaaatggc
     1261 cgacccgaag tgggtgatct cgatgggcgc ctgcgcgagc tcgggcggga ttttcgacaa
     1321 ctacgcgatc gttcagggga tcgataccat cgttccggtc gacgtctacg ttcccggctg
     1381 tccgcccacc cccgatggat tgctctacgc cgtcaacttg attcagcagc aaatccgcga
     1441 aggcggacgc ggcggaatcc tcgctcgctc gtagggaagt ttaaatgccc agcacgcaaa
     1501 caccgccgat cgcaggcgcc gcgatcgacc cgcccgcgct gcgcccgccg atcgacgacg
     1561 cgcagatcga acgcgtcgcg ccgagcgcgc tgcgcgcccg gctggaagcg ctgcgagccg
     1621 caggcttcac gatgctgctc gatctcggcg cggtcgacta cttgcagcgc gatccgcgtt
     1681 tcgacgtgat ctatcacctc ttgaagctgc cgccggcggc ggccggcgtc gccgaggtcg
     1741 gctcgcccgc gcgggtgcgt attttgtgcg gcgtaggcgg cgacgatccg cgcctcccga
     1801 ccgccatgga tctgtggaaa tcggccgatt gggcggagcg cgaaatcttc gtcctgtttg
     1861 gcatcacctt cgacggacac gccaatttgc ggcgcatcca aatgccctac gactgggaag
     1921 ggcatccgct gcggcgcgac tatccggtgc gcggtccggc gcgcgagcgt gcgccgcggc
     1981 ccgcgttcgc gctcaagagc aacgtctcgg ccggtacgcc gccgtcgggg acgacgctcg
     2041 aagcgcttca ggaacaggtc aaacgcgtgc gcgaaggaga cg
//
LOCUS       DASUCJ010000113         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1407259, whole genome shotgun sequence.
ACCESSION   DASUCJ010000113 DASUCJ010000000
VERSION     DASUCJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407259"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3201
                     /locus_tag="VFU90_01385"
     CDS             <1..3201
                     /locus_tag="VFU90_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5478448.1"
                     /translation="NSSSSEGNTRVTAQFYFGTNIDTAAVDVQEQVSRIWGTLPNDPN
                     LQQPVITKFDSNSLPVVRLFVTDPNMTLRDLGDLFTNQLSDEFSSIDGVAAVTVTNDQ
                     QRAIMVAPDSRELAVNGISLQQIMQRIAQENINLPAGVVQVGKSEYLVRASALLQSAE
                     ETGNLIVTTKNGAPIRLNQVAKVGDSILEQRSFQRLNGTPAVGITINAQPDANIVATA
                     VGVYKKIATLEQRYPGMHLAVVFDQQGYIREAISALEHTALYGAFLAILIILIFLHSW
                     RSTLIVAVSLPVSILGTLFAAYVFGFSLNIMTLGGLALAVGLIVDDAIVVIENIYRHM
                     ARGQTPMEAAETATAEIFSAVLASSITVITVFVPLVLIPGLQGLIFTPFAVMVMVAVG
                     LSLVVALTQVPMLSSVILRKRSAAPGNGSATNGRHRGAYARFAAGFDARYERFARWYA
                     RRLAQAVDRPMAVFAVAGIVLAVTVVAVELGAVKTELFPPSNSNFARFNLQTPTGTAL
                     SVTNAIATDVENRMRKDPRVADVGVLVGSSNPVSGTQVTNEANMSVTLKPSVSGSQAQ
                     QFVTQWTAALTGSRSASGGRGRASTSFTPEQLARLRARFGKPIPGLQVFGRTIDIVQN
                     IIARGQDALQIQIFGPDINTLYNVAEFTAIPRLQAEVQGLQPPEPGITNAQPEIDVNV
                     DRMEAAQLGVSTQTISQVIDIATAGQTASFMQINGTQYPIQVQLPPDQRRSLQAIQNL
                     MVPVSTIPNGLVVNTSSSLPVAATQNGALTLNTAAGTTGNYTLQTMPLAELAKITFGT
                     GPSEITRQNKQREIDIDAGLNIPLGQAVAQSTRVMNSITLPPGYYWAFGPQVQQQGDT
                     FSSLGLIVALAILLIYMLLASQFESVLHPLVIMVAVPLASAGVVFGILFRNLIWEALA
                     AITRQPFAPMAFGLTAFIGVLMLVGIVVKNAILVVEFSNQLRERGMSPRDAVLHAAPM
                     RLRPIMMTTLATIGGMTPIALGIEAGSQTQAPLGVVVIGGLAVSTTLSLIVVPTLYLW
                     AAQHVEPRLGGFHRGAEVKTVRGEEEFAEEPISAIE"
     gene            complement(3185..>3851)
                     /locus_tag="VFU90_01390"
     CDS             complement(3185..>3851)
                     /locus_tag="VFU90_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5478449.1"
                     /translation="FLSFPALVFAGVPAISANATNNAAMWVGTIGSARGYKEEVAAHR
                     GLLLPIVSASAAGSIVGALLLLLTPAPLFERLIPWLLVFATCTFAAAPWLTRRSDAPP
                     RHAPWQIAVQFLVAIYGGYFGAGMGILMLAVLAFSGLPTFNAQNAVKNVLAVAINGVA
                     LVPFAIARIVDWRFAVPMAAIALLGGYFGARFFRRVSPRISRPIVIAIGAAMTVAFFT
                     RWR"
BASE COUNT          696 a         1323 c         1198 g          634 t
ORIGIN      
        1 aactccagca gctccgaagg caatacccgg gtcaccgccc agttctactt cggcaccaac
       61 atcgacaccg cggccgtcga cgttcaggag caggtctcgc gcatctgggg cacgcttccc
      121 aacgatccca acctgcagca gcccgtcatc acgaagttcg attcgaattc gctgccggtc
      181 gtgcgcctgt tcgtcaccga tccgaacatg acgctgcgcg acctcggcga tctgttcacc
      241 aatcagctct ccgacgagtt ctcctcgatc gacggcgtcg ccgccgtcac cgtcaccaac
      301 gaccagcagc gggcgatcat ggtcgcgccc gattcgcgcg agctggcggt caacggcatc
      361 tcgctgcagc agatcatgca gcgcatcgcc caggagaaca tcaatcttcc ggccggggtc
      421 gtacaggtcg gaaagagcga gtacctcgtc cgcgcgagcg cgctgctgca gtcggcggaa
      481 gaaaccggca atctgatcgt cacgaccaag aacggcgcgc cgatccggct gaatcaagtt
      541 gcaaaggtcg gcgactcgat tctcgagcag cgcagcttcc agcggctcaa cggcacgccg
      601 gccgtcggca ttacgatcaa cgcccagccc gacgccaaca tcgtcgcaac cgcggtcggc
      661 gtttacaaga agatcgctac cctcgagcag cgctaccccg gtatgcatct ggcggtcgtc
      721 ttcgaccagc aaggctacat ccgggaggcg atctcggcgc tcgagcacac cgccctctac
      781 ggtgccttcc tcgcgatcct gatcatcctg atcttcctgc actcgtggcg ctcgacgctg
      841 atcgtcgccg tctcgctgcc ggtgtcgatc ctcggcacgc tgttcgccgc ctacgtgttc
      901 gggttttcgc tcaacatcat gacgctcggc ggcctggcgc tggccgtcgg gctgatcgtc
      961 gacgacgcga tcgttgtgat cgaaaacatc taccggcaca tggcccgcgg acaaacgccg
     1021 atggaagccg ccgaaaccgc gaccgcggag attttttcgg ccgtgctcgc ctcgtcgatc
     1081 acggtcatca cggtgttcgt gccgctggtg ctcatcccgg ggctgcaagg actgatcttc
     1141 acgccgtttg ccgtcatggt gatggtagcg gtcgggcttt cgttggtggt cgcactgacg
     1201 caagttccga tgctgtcgtc ggtcattctg cgcaagcgct ccgcggcacc cgggaacggc
     1261 tcggcgacga acggccggca tcgcggcgcg tacgcccgct ttgcggccgg cttcgacgcc
     1321 cgatacgagc gcttcgcgcg ctggtacgcg cgccggctgg cgcaggccgt cgatcgcccg
     1381 atggccgtct tcgccgtcgc cggaatcgtg ctggccgtca ccgtcgttgc ggtcgaactc
     1441 ggcgccgtca agaccgaact gttcccgccc tcgaactcca acttcgcgcg tttcaatctg
     1501 caaacgccca ccggcacggc gctgtcggtg acgaacgcga tcgcgaccga cgtggaaaac
     1561 cggatgcgca aagatccgcg cgtcgccgac gtcggcgtac tggtcggcag ctcgaatccg
     1621 gtcagcggaa cgcaagtgac caacgaagcc aacatgtcgg tcacgctcaa gccgagcgtg
     1681 agcggcagtc aagcgcagca gttcgtcacg cagtggacgg ccgcattgac cggttcgcgt
     1741 tcggcgtcgg gcggacgcgg acgcgcctca acgtcgttca cgcccgagca gctggcgcgg
     1801 ctgcgcgccc gtttcggcaa accgattccg ggactccaag tcttcggccg cacgatcgac
     1861 atcgtgcaga acatcatcgc gcgcggccaa gacgcgctgc agatccagat cttcggcccc
     1921 gacatcaaca cgctctacaa cgtcgccgag ttcaccgcga ttccgcggct gcaggccgaa
     1981 gtgcagggtc tccagccgcc cgagccgggg atcaccaacg cgcagcccga gatcgacgtc
     2041 aacgtcgacc ggatggaggc cgcccagctg ggcgtcagca cgcaaaccat ctcgcaggtg
     2101 atcgacatcg cgaccgccgg tcagaccgcc tcgttcatgc agatcaacgg gacgcagtat
     2161 cccattcagg tgcagctgcc gcccgaccag cgccgcagct tgcaggccat ccagaacttg
     2221 atggtgccgg tctcgaccat tccgaacggg ctggtcgtca acacgtcgtc gtcgttaccg
     2281 gtagccgcca cccaaaacgg cgcgctgacg ctcaacaccg ccgccggcac gaccgggaac
     2341 tacacgctgc agaccatgcc gctcgcggaa ctggccaaga ttacgttcgg cacggggcct
     2401 tcggaaatca cccgccaaaa caaacagcgc gaaatcgaca tcgacgcggg cttgaacatt
     2461 ccgctcggcc aggcggtcgc gcagtcgacg cgggtgatga actcgattac gctgcccccc
     2521 ggttactact gggcgttcgg gccccaagtt cagcagcagg gcgatacgtt ctccagcctc
     2581 gggttgatcg tagcgctcgc gatcctgctc atctacatgc tgctcgcctc gcagttcgag
     2641 tcggtgctgc acccgctggt gattatggtc gccgttccgc tggcgtcggc gggcgtcgtc
     2701 ttcgggattt tgttccgtaa cctcatttgg gaagcgctgg ccgcgattac gcgtcagccg
     2761 ttcgcgccga tggccttcgg tttgacggcc ttcatcggcg tgctgatgct ggtcggcatc
     2821 gtcgtcaaga acgccatcct ggtggtcgag ttcagcaatc agctgcgcga acgcgggatg
     2881 agcccgcgtg acgccgtgct gcacgccgcg ccgatgcggc tgcggccgat tatgatgacg
     2941 accctcgcca ccatcggcgg catgacgccg atcgcactcg gtatcgaagc cggcagccaa
     3001 acgcaggcgc cgctgggcgt cgtcgtcatc ggcgggctgg ccgtttcgac gacgctatcg
     3061 ctgatcgtcg tgccgacgct ctatctctgg gcggcgcagc acgtcgagcc gcggctgggc
     3121 ggcttccacc gcggcgccga ggtcaagacg gtacgcggcg aagaggaatt cgccgaagag
     3181 ccgatcagcg ccatcgagta aagaacgcga ccgtcatcgc ggcgccgatc gcgatgacga
     3241 tcgggcgcga gatgcgcggc gaaacgcggc gaaagaagcg cgcgccgaag tatccgccga
     3301 gcagcgcgat cgccgccatc gggacggcaa aacgccagtc gacgatccgc gcgatcgcaa
     3361 acggaacgag cgccacgccg ttgatcgcga ccgccagcac gttcttgacc gcgttttggg
     3421 cgttgaaggt gggcagtccc gaaaatgcca gcacggccag cattagtatc cccatgcccg
     3481 cgccgaagta gccgccgtaa atcgccacca gaaactgcac cgcgatctgc cacggagcgt
     3541 ggcgcggcgg cgcatccgag cggcgggtga gccacggcgc cgccgcaaac gtgcacgtcg
     3601 cgaacaccag cagccacgga atcagccgtt cgaagagcgg ggccggcgtc agcagcaaga
     3661 gcagcgcgcc gacgatcgat cctgccgcgc tcgcggagac gatcggcagc agcagcccgc
     3721 gatgcgcggc gacttcttct ttgtagccac gggcgctgcc gatcgtgccg acccacatcg
     3781 ccgcgttgtt ggtggcgttg gccgagatcg ccggcacccc ggcgaacacc agcgccggaa
     3841 acgacaggaa g
//
LOCUS       DASUCJ010000114         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_307065, whole genome shotgun sequence.
ACCESSION   DASUCJ010000114 DASUCJ010000000
VERSION     DASUCJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307065"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFU90_01395"
     CDS             <1..1117
                     /locus_tag="VFU90_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5478450.1"
                     /translation="DPNNPSKQITIKLVRSSLRSKLTPANGYQQFVIEYDGGKMDGFN
                     VPPVEGHPGTYVYQYVNPSDVVPYWDLAKQYVLADRMFQTQGSGSFTAHQDLIAGGTL
                     IDPTHALIDNPSNQPWGCDAVPGTVTSLITTSKQFLQGQGPFPCLSYKTLRDLLDARG
                     VSWKYYTPPIGQSFAGNIWNAFDAIHDVRYGSEWKSNVVDVATLLPDISKGNLPSVAW
                     VCPDFNNSDHPGAPSDTGPSWVAQVVNAIGNGPDWDTTAIVVLWDDWGGFYDHVAPPQ
                     LDYQGLGFRVPMLIVSPYAKQGVVSNTQYEFGSIVKFVEDNWNLGRLGTTDVRANSID
                     DAFDFQKPPRPFSTIGAKYSRAFFLHQRPSNHPLDNG"
     gene            1197..>3277
                     /gene="gyrA"
                     /locus_tag="VFU90_01400"
     CDS             1197..>3277
                     /gene="gyrA"
                     /locus_tag="VFU90_01400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5478451.1"
                     /translation="MNNDIIQQVNVEDEMRESYLSYAMSVIASRALPDVRDGLKPVQR
                     RILYAMREMGFDPTKQHRKCAGIIGEVLKSYHPHGDSSVYDALVRLAQDFTLRYPLVD
                     GHGNFGSIDPDPPAAYRYTEARLARTALEVLADIDKETVGFVPNFDNQGTEPSVLPGR
                     LPQLLVNGSSGIAVGMATNIPPHNLGEIADAVAALIDDPETTDDALSDIVRGPDFPTG
                     GTILGHEAIREAYKTGRGSIAIRGKAEIVEDRGRHKIVITEIPYQVYKSRIIEAIAEA
                     HSEKRIQGIARLDDESNRKGMRVVVELQKTATPKVVLNQLFKHTPLQASFGFNMLALV
                     PVGEPRADGSVAIEPQVLSLKQLLEHFIAHRRNVVTLRTRYELRKAEERAHLLEGYRI
                     ALDNIDEVIEIVRGSQTTDEAKQRLSARFSFTDVQAQAIVDMRLRTLVGLERQKIEDE
                     YSELIKTIAELQDILNSPRRVGQIVKSETLDLKKRFGDARRTSIEPAEDEISMEQLIP
                     NIDVVVTYTVGGYIKRVSLDTFRTQNRGGRGVIGIANLKREDVVRNFFVTKTHDHVLF
                     FTNKGRAYRLRGYEIPDTTRTARGTALVNLLTLPPGEHVTAVFPIDKFEGEKYMVMVT
                     KDGVIKKTKLDEFSNVRRNGLNAINLDENDELMAVDLSDGTRDIILASTAGMAVHFNE
                     KDVRPMGRNARG"
BASE COUNT          688 a         1037 c          980 g          572 t
ORIGIN      
        1 cgatccgaac aatccgagca agcaaattac gatcaagctc gtgcgaagca gccttcgcag
       61 taagctcacc cccgccaatg gctaccagca gttcgtgatc gagtacgacg gcgggaagat
      121 ggatggattc aacgttccgc ccgtcgaggg acatccggga acctacgtct atcagtacgt
      181 caatccgtcg gacgtcgtgc cgtattggga tttggccaaa cagtacgtgc ttgccgaccg
      241 gatgtttcaa acgcagggca gcgggagttt tacggctcac caggatctga tcgccggcgg
      301 aacgctgatc gatccaacgc acgcgctgat cgacaatccg agcaaccagc cgtgggggtg
      361 cgacgcggtg ccggggacgg ttacgtcgct gattaccacc agcaaacagt tcttgcaggg
      421 tcagggcccg tttccgtgcc tgagctacaa aacgctgcgc gaccttctcg atgccagagg
      481 cgtgagctgg aagtactaca cgccgccgat cggccagagc tttgccggca acatttggaa
      541 tgcgttcgac gcgatccacg acgtccgtta cggctcggaa tggaagtcca acgtcgtcga
      601 cgtagcgacc ctgctccccg acatctccaa aggaaatctt ccgtcggttg cgtgggtgtg
      661 cccggacttc aataattccg accacccggg ggcgccttcg gataccgggc cttcgtgggt
      721 ggcgcaggtg gtaaacgcga tcggcaacgg tcccgactgg gacaccaccg cgatcgtcgt
      781 gctgtgggac gattggggcg gattttacga tcacgtggcc ccgccgcagc tcgactacca
      841 aggcctcggc ttccgcgtcc ccatgctgat cgtgtcgccg tacgccaagc aaggcgtggt
      901 ttcgaacacc cagtacgaat tcggcagcat cgtgaagttc gtcgaggata actggaattt
      961 gggccggctg ggtactaccg acgtacgggc gaactcgatc gacgacgcgt tcgactttca
     1021 aaagccgccg cgcccgtttt cgacgattgg ggcgaaatat tcgcgcgcct ttttccttca
     1081 ccagcggccg tcgaatcatc ccctcgacaa cggatagctc ccgcttcggg caagggaatt
     1141 ccgggctccg gcggaatagt ccgcctttgc attttttgcc cgaccttacg taaaacttga
     1201 acaacgacat catccaacaa gtcaacgtcg aagacgaaat gcgggagagt tatctctcgt
     1261 atgcgatgtc cgtcattgcg tcgcgcgctc tccccgacgt gcgcgacggg ctcaaaccgg
     1321 tccagcgccg catcctctat gcgatgcgcg agatgggctt cgatccgacg aagcaacatc
     1381 gcaagtgcgc cggtatcatc ggcgaggttc taaagagcta tcacccgcac ggcgactcgt
     1441 cggtgtacga cgcgctcgtg cgtttggcgc aggatttcac gctccggtat ccgctcgtcg
     1501 acgggcacgg aaacttcggg tcgatcgacc cggatccgcc ggccgcttac cgctataccg
     1561 aggcgcgcct cgcgcggacc gcgctcgagg ttctggccga catcgacaaa gaaaccgtcg
     1621 gattcgttcc caatttcgac aatcagggga cggagccgag cgtgctgccg ggacgcctcc
     1681 cgcagctgct cgtcaacggc tcgtcgggca tcgcagtcgg aatggcgacc aacatcccgc
     1741 cgcacaatct cggggaaatc gccgacgcgg tggcggcgct gatcgacgat cccgaaacca
     1801 ccgacgacgc gctgagcgac atcgtgcgcg gccccgattt tccgaccggc gggacgatcc
     1861 tcggccacga ggcgatccgg gaagcgtaca aaacgggccg cggttcgatt gcgattcgcg
     1921 gtaaggcgga aatcgtcgag gatcgcggcc ggcacaagat cgtcatcacc gagatcccgt
     1981 atcaagttta taaatcgcgc atcatcgagg cgatcgcgga agcgcactcg gagaagcgca
     2041 ttcaaggcat cgcgcggctc gacgacgagt cgaaccgcaa aggcatgcgg gtcgtcgtcg
     2101 agctgcagaa gacggcgacg cccaaggttg tgctgaacca gctcttcaaa cacacgccgc
     2161 tgcaggcgtc gttcggcttc aacatgctgg cgctcgtgcc ggtcggcgag ccgcgcgccg
     2221 acggatcggt cgcgatcgaa cctcaggttc tctcgctcaa acagctcctc gagcatttca
     2281 tcgctcaccg gcgcaacgtc gtcactctgc gaacgcgcta cgagctgcgc aaggccgaag
     2341 agcgcgcgca ccttctcgaa ggctaccgga tcgcgctcga caacatcgac gaagtcatcg
     2401 agatcgtgcg cgggagccag acgaccgacg aggcgaagca acggctgtcg gcgcgcttct
     2461 cgttcaccga cgtgcaggcg caagccatcg tcgacatgcg tctgcggacg ctcgtgggct
     2521 tggaacgcca gaaaatcgag gacgaatact ccgagctgat caaaacgatc gccgaactgc
     2581 aggacattct caatagtccg cggcgcgtgg ggcagatcgt caagagcgag acgctcgacc
     2641 tcaagaagcg gttcggcgac gcgcgccgca cgtcgatcga gccggccgaa gacgagatct
     2701 cgatggagca gctgataccg aacatcgacg tcgtcgtcac ctacacggtc ggcggctaca
     2761 tcaagcgcgt ctcgctcgac acgttccgca cgcagaatcg cggcggccgc ggcgtcatcg
     2821 gcatcgccaa cctcaaacgc gaggacgtcg tccgcaactt cttcgtaacg aagacccacg
     2881 accacgtgct gttcttcacg aacaagggcc gcgcgtatcg cctgcgcgga tacgagattc
     2941 ccgataccac gcgcaccgcg cgcggcacgg cgctcgtgaa cctgctcacg ctcccgccgg
     3001 gcgagcacgt caccgcggtc ttcccgatcg acaagttcga aggcgagaag tacatggtga
     3061 tggtcaccaa agacggcgtc atcaagaaga ccaagctcga cgaattctca aacgttcgcc
     3121 gcaacggttt gaacgcaatt aacctcgatg aaaacgacga gttgatggcg gtcgatctct
     3181 ccgacggcac gcgcgatatc atcctcgcgt cgaccgccgg catggccgtg catttcaacg
     3241 agaaagacgt tcgcccgatg ggtcgcaacg cgcgcgg
//
LOCUS       DASUCJ010000115         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_490277, whole genome shotgun sequence.
ACCESSION   DASUCJ010000115 DASUCJ010000000
VERSION     DASUCJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490277"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>1202)
                     /locus_tag="VFU90_01405"
     CDS             complement(169..>1202)
                     /locus_tag="VFU90_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478452.1"
                     /translation="DERHELFAFTPDRLLLLLHDARDLRRYRDTTIGGAAETAVSATI
                     DHYPTTVFFRRGDGFLTMARYQADESNDFGLAPWGPMSVEEWYSVWRYDSVANVRSPS
                     EWDIVRIGKPYKRMTILSVAYNPSLPADSLVLGDSIRAEYLAHARRPMADLPLTRAHL
                     AAGGRIAVFATPGAPIAAVKVGRDWLLLEPGNLPLNAKRAAAWLAANDSGGKVVGAIL
                     GTAFPSGGASWVAKQRLPVYVSPAGAAGDSVSLRNYGAPLAALRPIARGQWIKPTNAA
                     RDSVWIEPIDVPNLQRALLVYVPSLRWAYSSAVSERAQCTAMAKQLRERGWRIDRFGL
                     PRSPVGVEP"
BASE COUNT          221 a          404 c          384 g          193 t
ORIGIN      
        1 agatcgtcgg ttgttgggcg gcgagaggcg cggcggctct cacgtgagtg tcgggcgcga
       61 ggccttgccg ggctttggcc caattgtgat gcctccacgc ctcgatgtgt ccgcgggcga
      121 catcccgacg cggcgtcctg gctcgttcgc gcacaatgga tccgcggctt acggttccac
      181 accgacgggc gagcgaggca agccaaagcg atcgatcctc cacccccgct ctcgcagctg
      241 ctttgccatc gccgtgcact gcgcgcgctc cgagaccgcg ctcgagtatg cccaacggag
      301 cgatggcaca tacacgagca gcgcgcgctg caggttcggg acgtcgatcg gctcgatcca
      361 gactgaatct cgcgccgcgt tagtcggctt gatccattga ccgcgcgcaa tcggccgaag
      421 agccgcgagg ggcgcaccgt aattcctgag cgacacggaa tcgccggcgg caccggcggg
      481 cgatacgtac acgggcagtc gctgcttcgc tacccacgac gcgccgcccg acggaaacgc
      541 ggtgccgagt atcgccccga cgaccttccc gcccgagtca ttcgcggcga gccaagctgc
      601 cgcacgcttg gcgttgagcg gcagattgcc aggttccaac agcagccaat cgcgaccaac
      661 cttgaccgcg gcgatcggtg caccgggcgt ggcaaacacg gcgatccgtc cgccagccgc
      721 caagtgcgcg cgggtaagcg gcagatccgc catcggacgt cgggcgtgtg ccagatattc
      781 ggcgcgaatc gaatcgccta acacgagcga gtcggcgggc aacgacggat tgtacgcgac
      841 cgagagaatc gtcatgcgct tgtacggttt cccgatgcgc acgatgtccc attccgatgg
      901 acttctgaca ttggccacgg agtcgtatcg ccagaccgaa taccactcct cgaccgacat
      961 cggtccccac ggagccagac cgaagtcatt ggattcgtcc gcctgatacc gcgccatcgt
     1021 gaggaaccca tcgccacgtc gaaagaacac cgtagtcggg tagtggtcga tcgtcgcgga
     1081 gacagcggtc tccgccgcgc cgccgatcgt cgtgtcgcgg tagcggcgca ggtctcgcgc
     1141 gtcgtgcaac agcaacagca atcgatcagg cgtgaacgcg aagagctcgt gccgctcgtc
     1201 aa
//
LOCUS       DASUCJ010000116         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_688887, whole genome shotgun sequence.
ACCESSION   DASUCJ010000116 DASUCJ010000000
VERSION     DASUCJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688887"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2169
                     /gene="ligD"
                     /locus_tag="VFU90_01410"
     CDS             <1..2169
                     /gene="ligD"
                     /locus_tag="VFU90_01410"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU5478453.1"
                     /translation="LIKERDAYVASPGDQFPEQSVLSGLTVEEVKAGGLKTKGESVRV
                     ELDDTEARRGRVDPRTVEVMLAEPTDAAFTRDGWIFELKLDGYRLIASKEHGEPLLLT
                     RNGNDYTAVFPEVARAIAALPLDSCIVDGEVVVLDPQGKPSFARLQQRGRLTSTLDIK
                     RAAVELPATFYVFDFLAFDDFDLRPLPLVKRKALLAESLPKLGAVRVLDHIERDGERF
                     LEQVAALGLEGVIAKRADSTYRGGRTDSWLKIKAEKTGDFVIVGFTAPKGGRGHFGAL
                     QLADMVNGTLTYAGRAGTGFDDELLGEIGRMLAPIVRPDPPCAGPAVEPGAEPRPSTD
                     IPETRTTTWVEPRYVCEVRFREWTPDGVLRHPAFLHLRADKNPEDCDRQGWNAAPRVA
                     ATRDTPARDAAGGADAPPPVAPQPEEKTVAFSNLKKVYWPAERYTKGDLIEYYRAVSF
                     WLLPYLRNRPVVLTRYPDGIDGKMFYQKDAPEFAPRWIRTIPIWSEDTQREIRYFVCD
                     DEETLLYIANMGSIPLHIWASRVSSLEQPDWCVIDLDPKEAPFSDVVRTAIVLRRLCE
                     SIELPSYVKTTGKTGLHILVPLGRQMTYAQSRTLGELLARVVLRESHGYATIIRHVTR
                     RGDKVYLDYLQNRHGQTIVAPFSVRPLPGATVSMPLVWDEVNDALDPKAFTIRNALER
                     MERLGGDPCAAVLEQKPDLGAALQRLSAVLAQGQDPADEP"
     gene            complement(2246..>2697)
                     /gene="msrA"
                     /locus_tag="VFU90_01415"
     CDS             complement(2246..>2697)
                     /gene="msrA"
                     /locus_tag="VFU90_01415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5478454.1"
                     /translation="GVISTRVGYTGGDVPNATYRNHGTHAEAIEIIFDPATTSFRKLL
                     EFFFQIHDPTTRNRQGNDIGMSYRSAIFYTTPEQQRVARDTIADVDASGLWPGKLVTD
                     VVPAGPFWEAEPEHQDYLERIPNGYTCHFVRPGWVLPKRGETVASGA"
BASE COUNT          442 a          888 c          951 g          416 t
ORIGIN      
        1 ctcatcaaag agcgcgacgc gtacgtcgca tcgccgggcg accagttccc ggagcagtcg
       61 gtgttgtccg gcctaacggt ggaagaagtg aaggccggcg gcctcaagac caagggcgaa
      121 tcggttcgcg tcgagctcga tgacaccgag gccaggcgcg ggcgcgtcga cccgcgcacg
      181 gtcgaggtca tgctggccga gcccacggat gccgcgttca cgcgcgacgg atggatcttc
      241 gagctcaagc tcgatggcta ccggctcatc gccagcaagg agcacggcga gccgctgctg
      301 ctcacgcgca acggcaacga ctacacggcc gtgttccccg aagtcgcgcg cgccatcgcc
      361 gcgcttccgt tagacagttg catcgtggac ggcgaggtcg tggtgttgga cccgcagggg
      421 aagccgagct tcgcgcggct gcagcagcgc ggccggctga cgtcgacgct cgacatcaag
      481 cgcgctgccg tcgagctgcc ggccaccttc tacgtcttcg attttctcgc gttcgacgac
      541 ttcgacctgc ggccgctgcc gctggtcaaa cgcaaggcgc tgctcgccga gtcgctgccc
      601 aagttaggcg cggtgcgcgt gctcgatcac atcgagcgcg acggcgagcg gttcctcgag
      661 caggtggcgg ccctcggcct ggaaggcgtg atcgccaagc gcgccgattc gacgtatcgc
      721 ggcggccgca ccgactcgtg gctcaagatc aaagcggaga agacgggcga cttcgtgatc
      781 gtcgggttca ccgcgcccaa gggcggtcgc gggcacttcg gcgcgctgca gctggccgac
      841 atggtgaacg gcacgctcac ctacgccggc cgcgccggca ccggcttcga cgacgagctg
      901 ttaggcgaaa tcggccgcat gctggcgccg atcgtgcggc ccgatccgcc gtgcgccggg
      961 cccgccgtcg agccgggcgc cgaaccgcga ccgtccacgg acattcccga gaccaggacg
     1021 acgacgtggg tcgagcccag gtacgtctgc gaggtgcgct ttcgcgagtg gacaccggac
     1081 ggcgtgctgc gccatccggc gttcctgcac ctgcgcgccg acaagaaccc ggaggattgc
     1141 gaccgtcagg gctggaacgc ggcgccgcgc gttgcggcga cgcgcgatac gccggcgcgc
     1201 gatgcggccg gcggcgctga tgcgccgccg ccggtcgcgc cgcagccgga ggagaaaacg
     1261 gtcgcgttct cgaatctgaa gaaggtgtac tggccggccg agcggtacac caagggcgat
     1321 ctcatcgaat actatcgggc ggtgtcgttc tggctgctgc cgtacctgcg caaccggccg
     1381 gtggtattga cgcgctatcc ggacgggatc gacggcaaaa tgttctacca gaaagacgcg
     1441 cccgagttcg caccgcgctg gattcggacg attcccatct ggagcgagga cacacagcgc
     1501 gagattcgct acttcgtgtg cgacgacgaa gagacgctgc tctacatcgc gaacatggga
     1561 tcgatcccgc tgcacatctg ggcgagccgg gtgagctcgc tggagcagcc cgactggtgt
     1621 gtgatcgacc tcgacccgaa agaagcgccg ttctcggacg tcgtgcgcac ggcgattgtg
     1681 ctccgccgcc tctgcgagtc gatcgagctg ccgagctacg tcaagacgac gggaaagacc
     1741 ggcttgcaca tcctggtgcc gttgggccgg cagatgacgt acgcgcagtc gcgcacgtta
     1801 ggcgagctgc tcgcgcgcgt ggtgctccgc gagagccacg gctacgcgac catcatccgc
     1861 cacgtgacca ggcgcggcga caaggtctat ctcgactatc tgcagaaccg tcacggacag
     1921 accatcgtgg cgccgttcag cgtgcggccg ctccccggcg cgaccgtgtc gatgccgctc
     1981 gtctgggacg aggtgaacga cgcgctcgac cccaaggcgt tcacgatccg gaacgcgctc
     2041 gagcggatgg agcggttagg cggcgacccg tgcgcggcgg tcctggagca gaagccggat
     2101 ctgggcgcgg cgctccagcg gctgtcggcg gtgctcgcgc agggccagga tccggccgac
     2161 gagccgtgag ccgcggcgaa ggacacgccg gcgtgcgccg actgcagccg cccgcgcgcc
     2221 taacgaagtc gtggcgccgc gccgctcacg cccccgacgc gaccgtttcg ccgcgcttcg
     2281 gcaggaccca gccgggccgg acgaagtgac aggtgtagcc gttggggatc cgctcgagat
     2341 agtcctgatg ctcgggttcc gcttcccaga aggggcccgc cggcacgacg tccgtcacca
     2401 acttgcccgg ccacaagccg gacgcgtcga catcggcgat ggtgtcgcgg gcgacgcgct
     2461 gctgctccgg cgtggtgtag aagatcgccg agcgatagct catcccgatg tcgttgccct
     2521 ggcggttgcg cgtggtcggg tcgtgaatct ggaagaagaa ttcgagcagc ttccggaagc
     2581 tggtcgttgc cggatcgaag atgatctcga tcgcttcggc gtgggtgccg tggttgcggt
     2641 aggttgcgtt aggcacgtcg ccgcccgtgt agccgacgcg agtcgagatg acgccgg
//
LOCUS       DASUCJ010000117         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_444533, whole genome shotgun sequence.
ACCESSION   DASUCJ010000117 DASUCJ010000000
VERSION     DASUCJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444533"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VFU90_01420"
     CDS             complement(<1..2096)
                     /locus_tag="VFU90_01420"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478455.1"
                     /translation="MRTSISRLILFLLAAAIAPATARTQSTDLYSDMHWREIGPTRAG
                     RARAVSGAASQPNVAYIGFDNGGVWRSTDYGSTWTPIFDHQSTGSIGAIAVAPSNPNI
                     IYVGTGAGIIRPDLATGNGMYKSTDAGKTWTPIGLDDSQMIAAVDVDPFNPDRVFVAA
                     LGHPYGPNEQRGVFRSTDGGHTFTKVLYKDEYTSANDVHIDPSNPMVVYASLWQQQEE
                     FREGGGFGGNSGGLFKSTDGGTTWTELKDGLPASVLEANIAISRSNPNVVYAMVAQPS
                     NRPGVVAAGYGAGVVAFYKSSDAGAHWTLVDGPGLTSRPVDPRPLVRIGGGDLPTIIV
                     DPKDENVVYSSSTVLWRTEDGGITWSAVRGAPGGDDYQKTWINPLNPNIIIQVSDQGG
                     VVSANRGQSWSNWYTQPTAAMYHVSTDNTFVYRVCGGQQDSGSACVKSRSDDGEITFH
                     DWHPVNIQEYGVAAPDPRDPDLVYGSMRTNVSLYNRTTGQTTYVGPDMSAPGPNGDTY
                     NRNVRTMPIVWSPEDPNVLYYTSNVVWKSADHAHSWTRISPDLARQTWTVPASAGKYA
                     TDVTASPLGTITALAPSPRSARVLWAGTDDGNIQVTTNGGVTWTNVTPSAIKPWTRIF
                     NIDAGHFDDRTAYAAANTMRIDDANPHFFRTHDGGKTWTEIDNGIAPGAAANSIREDP
                     RQKGLLYAATETQVWVS"
     gene            2258..2719
                     /locus_tag="VFU90_01425"
     CDS             2258..2719
                     /locus_tag="VFU90_01425"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5478456.1"
                     /translation="MTTAELLAFMRGNKLAVQASVSTTNAAQAAVVGIAVTDALEVVF
                     DTLDSTRKVANLRTNPQLAFVIGGPVPGDERTVQYEGVADEPTGPELERIRAAYFSAW
                     PDGRSRANWPGLIYVRVRPTWIRYSDFNQHPPLVVEFDAGRLNVGASQAVR"
     gene            complement(2759..3070)
                     /locus_tag="VFU90_01430"
     CDS             complement(2759..3070)
                     /locus_tag="VFU90_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478457.1"
                     /translation="MYLIRDIMYCKPGKVRPMVEKFIAMSKLGEKFGGPRMKVMTDMC
                     AEQYWTVVAEMEVPDLKTFEEMMSPKTMSADDRKQFEEVMKGYHDLVDHGKREIYTIE
                     A"
BASE COUNT          535 a         1079 c         1168 g          621 t
ORIGIN      
        1 gacacccaga cctgcgtttc ggttgccgcg tagagcagtc ctttctgccg cggatcttcg
       61 cggatcgagt tcgcagcggc gcccggcgcg atgccgttgt cgatttccgt ccacgtcttg
      121 ccgccatcat gcgtgcggaa gaagtgcggg ttcgcgtcgt cgattcgcat cgtgttggcg
      181 gcggcgtacg cggtgcgatc gtcgaagtgg ccggcgtcga tgttgaagat gcgcgtccac
      241 ggcttgatgg cggacggtgt gacgttggtc caggtgacgc cgccgttcgt ggtaacctgg
      301 atgttgccgt cgtcggtgcc ggcccagagc acgcgggcgg accgcggcga cggcgcgagg
      361 gcggtgatgg tgcctaacgg cgatgcagtg acgtccgtcg cgtacttgcc ggcggatgca
      421 ggcacggtcc aggtctgccg ggcgagatcg gggctgatgc gcgtccacga gtgcgcgtgg
      481 tccgccgatt tccagacgac gttggacgtg taatagagca cgttgggatc ttcgggcgac
      541 cacacgatgg gcatcgtgcg gacgttgcga ttgtacgtgt ctccgttagg cccgggcgcg
      601 ctcatgtcgg ggccgacgta ggtcgtctgg ccagtcgtgc gattgtagag cgagacgttg
      661 gtccgcatgc tgccgtacac gagatccggg tcgcgcggat cgggcgcggc gacaccgtac
      721 tcctggatgt tgaccgggtg ccagtcgtgg aacgtgatct cgccgtcgtc ggagcggctc
      781 ttcacgcagg cggagcccga gtcctgctgg ccgccgcaca cgcggtacac gaacgtgttg
      841 tcggtggaga cgtgatacat cgcggcggtc ggctgcgtgt accagttgct ccacgactgg
      901 ccgcgattgg cggacaccac gccgccctgg tcgctcacct ggatgatgat gttcgggttg
      961 agcggattga tccacgtttt ctgatagtcg tcgccgccgg gcgcgccgcg cacggcggac
     1021 caggtgatgc cgccatcctc cgtgcgccag agcacggtgg aggagctgta caccacgttc
     1081 tcatccttgg gatcgacgat gatggtcggc aggtcgcccc cgccgatgcg gactaacgga
     1141 cgcgggtcga cggggcggct cgttaggccg ggaccgtcga cgagcgtcca gtgcgcgccg
     1201 gcgtcgctgg acttgtagaa cgcgaccacg ccggcgccgt agccggcggc gacgacgccc
     1261 ggccgattgg acggctgcgc gaccatcgcg tagacgacgt tcggattgct gcgggaaatc
     1321 gcgatgttgg cctcgagcac agacgctggc agcccgtcct tgagctccgt ccacgtcgtg
     1381 ccgccgtccg tggacttgaa caggccgccc gagttgccgc cgaacccgcc accttcgcgg
     1441 aattcctcct gctgctgcca gagggacgcg tacacgacca tcggattgga cggatcgatg
     1501 tgcacgtcgt tggcgctcgt gtactcatcc ttgtacaaca ccttggtgaa cgtatggccg
     1561 ccatcggtcg atcggaacac gccgcgctgt tcgttaggcc cgtagggatg gccgagcgcg
     1621 gcgacgaaga cgcggtcggg attgaacggg tcgacgtcca cggccgcgat catctggctg
     1681 tcgtcgaggc caatcggcgt ccacgtcttg ccggcgtcgg tggatttgta catgccgttt
     1741 ccggtcgcga gatcgggtcg aataatgccg gcgccggtgc cgacgtagat gatgttcggg
     1801 ttggacggcg caaccgcgat cgccccgatc gagccggtcg actggtggtc gaagatgggc
     1861 gtccaggtcg agccgtagtc ggtcgagcgc cacacgccgc cgttgtcaaa tccaatgtac
     1921 gcgacgttgg gctgactggc cgcacccgac acggcgcgtg cgcgaccggc ccgcgttggg
     1981 ccgatctcgc gccagtgcat gtccgaatac agatcggtcg actgagtgcg cgccgtggcc
     2041 ggtgcgatgg ccgcagcgag gaggaaaagg atcaggcgag agatggacgt gcgcacgaaa
     2101 acggcctcga cgatagagcg aaaagaatga tgacgtgaag tccgtgaccg cgaggaatat
     2161 ggggctggga cacgcgcgcg gctatgcagg agcacgccgc gattgcgccc catgttcgcc
     2221 tgttctatcg tagcggagcc ttcctccggt ggtgcgcatg acgacggccg agctcctcgc
     2281 gttcatgcgg gggaacaaac tcgctgtgca agcgtcggtg tcgacgacga acgcggcaca
     2341 ggcggccgtg gtgggcatcg cggtcaccga tgcgctcgag gtggtcttcg acacgctcga
     2401 ctcgacgcgc aaggtggcga atctgcgcac gaatccgcag ctcgcgttcg tgatcggcgg
     2461 gccggtgccc ggcgacgaac gcacggtgca gtacgaaggc gtggcggacg agccgacggg
     2521 cccggagctc gagcggatcc gcgcggcgta tttcagcgcg tggcccgatg gccgctcgcg
     2581 cgccaactgg cccgggctca tctacgtgcg cgtgcgtccg acctggatcc gctacagcga
     2641 tttcaaccag cacccgccgc tggtggtcga attcgacgcg ggccggctga acgttggagc
     2701 gtctcaggct gtgcgttagg cgaacagacg cggggcgcgg ctcgcgcccc ggctcccgtt
     2761 aggcctcgat cgtgtagatc tcccgcttgc cgtggtcgac cagatcgtgg tagcctttca
     2821 tgacctcctc gaactgcttc cgatcgtcgg ccgacatcgt cttcggcgac atcatctcct
     2881 cgaacgtctt gaggtccggc acctccatct cggcaaccac cgtccagtat tgctcggcgc
     2941 acatgtcggt catgaccttc atgcgcggac cgccgaattt ttcgccgagc ttcgacatgg
     3001 cgatgaactt ctcgaccatc ggccgcacct tgccgggctt gcagtacatg atgtccctga
     3061 tgagatacat gattccctcc cgccccagac gttgacgtag tcggactctc tccacctatt
     3121 taccacgacg cggagatacg tcaacgatgc gcggcctgca cgcgcgtacg gttcgcaccg
     3181 gaaacaggcg agccggacat gtcgacgcgc gctgtcccga cgggcgcata tcgcgcagcc
     3241 gcgagtccac cgcttccctt gcccccgccc atgcgcgcat ccgtcttcgt cggcaccagc
     3301 ctcgacggct tcatcgcccg cgtcgacggc agcctcgact tcctgccccc gggcggcggc
     3361 gagccgcacg gctacgagga attcatggcc acggtggacg cgc
//
LOCUS       DASUCJ010000118         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1422664, whole genome shotgun sequence.
ACCESSION   DASUCJ010000118 DASUCJ010000000
VERSION     DASUCJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422664"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1147
                     /locus_tag="VFU90_01435"
     CDS             125..1147
                     /locus_tag="VFU90_01435"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5478458.1"
                     /translation="MRKTLTLASLAAAAAVAGCANTSGNTAVTCSAVTATDSQPSPEW
                     QGTVFTIVMENKSQSDILGNKDAPFINSLAAQNAIAAGYRDNLVHPSEPNYLWMIAGE
                     NFGVLDDNDPSSHHIDSTSHIADQLDAAGLSWKAYAEGMGQPCDVNSHDLYAAKHNPL
                     VFFDDLNGWDGKQFNPTQRCMDRVVDFSQLSADVASGTLPKYVFIAPNMIDDMHDGTV
                     ADGDKWLANVLPPILSSDAFNKGGVIFLMWDEGGGYPQSDNPPMIVISPHAKPGYVST
                     VPYDTSSYLKTVQTVLGVQSLPCDASADSVQTMSDLFDVPLTPVPSSSPAATAAVDGG
                     AGGGGA"
BASE COUNT          226 a          455 c          388 g          199 t
ORIGIN      
        1 gacgactggg tcgaagcgtt caagcggcgc cgcgccgaga tcgtcgacca ccgctgcccc
       61 gggtagcgag cgagcccccg cccgctgttg cagcctgcgt gtgccgggtc atgctttgca
      121 caccatgcgc aaaacgctca cgctcgcttc cctggccgcc gccgccgctg tcgcgggctg
      181 cgccaacacg tccggcaaca cggcggtcac ctgcagcgcg gtcaccgcca cggactcgca
      241 gccgtcgccc gagtggcagg gcaccgtgtt caccatcgtc atggagaaca agagccaaag
      301 cgacatcctc ggcaacaagg atgcgccgtt catcaactcg ctcgcggcgc agaacgccat
      361 cgccgccggg tatcgcgaca acctcgtgca cccgagcgag ccgaactact tgtggatgat
      421 cgccggcgag aacttcggcg tcctcgacga caacgatccg agctcgcacc acatcgactc
      481 gacgtcgcac atcgccgatc agctcgacgc ggccgggctg tcctggaagg cgtacgccga
      541 gggcatgggg cagccctgcg acgtcaacag tcacgatctc tacgcggcca agcacaaccc
      601 gctcgtgttc ttcgacgatc tcaacggctg ggacggcaag cagttcaacc cgacgcagcg
      661 gtgcatggac cgcgtcgtcg acttttcgca gctcagcgcg gatgtcgcct cgggcacgct
      721 gccgaagtac gtcttcatcg cgcccaacat gatcgacgac atgcacgacg gcaccgtcgc
      781 cgacggagac aagtggctgg ccaacgtgct gccgcccatc ttgtcttccg acgccttcaa
      841 caagggcggc gtcatcttcc tcatgtggga cgaaggcggc ggctatccgc agtcggacaa
      901 cccgccgatg atcgtcatct cgcctcacgc caagcccggc tacgtctcga ccgtgccgta
      961 cgatacgagc tcgtacctga agacggtgca gacggttctc ggcgtgcaat cgttgccgtg
     1021 cgacgcttcg gcggacagcg tgcagacgat gtccgatctg ttcgacgtgc cgctcacgcc
     1081 cgtgccgtcc tcgtctcctg ccgccactgc cgccgtggac ggcggcgcgg gcggcggcgg
     1141 ggcgtagcta gaacgtaatt cccatcgtgc tcggcgcctg cgcgacgtgg tccgcgcgcc
     1201 aacgcttgta atgcgaagcc tgaacgagcc cgatcggcaa caggatcacg cccgccgcga
     1261 acgacgcc
//
LOCUS       DASUCJ010000119         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1086424, whole genome shotgun sequence.
ACCESSION   DASUCJ010000119 DASUCJ010000000
VERSION     DASUCJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086424"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1272)
                     /locus_tag="VFU90_01440"
     CDS             complement(<1..>1272)
                     /locus_tag="VFU90_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478459.1"
                     /translation="GASELFVNANTQSDARGLDAPTFDPIHDNSSQSDQFARFVTALG
                     PRATLAGDYSNQLAQFQIPINTDPNNPYDPIYTPAGTDDVQREYDRFANLNYTQTSRD
                     GNGVFQVIPWWRSTRINYDGDLPNDVLGLTPNFGSGIVCTSCATFGDPSLPAHNVGLL
                     SNTYANYVGLRAADFRATENHAWKVGFDIDRSNLEATQAFGCYYAGCAGSGVHAQPYY
                     LVASPEQAQAGSQVGIFAEDKWQLSKYAQINYGLRYDHSTGYTGGWQFSPRIGLNLWD
                     GGKNTFHAYYGRFYAAPLLEDVRQDCVVLQGCPSTPVYNLQPERDAYAEMGMDHVFDS
                     GLTGSINVFRKTVVNVLDTTQLLDTPLFAVFNNAVGLDTGVEVRLNGRALNGNSWYVN
                     GTVSGSYAACVSGSTFLFPPNVNPANQSCAS"
BASE COUNT          226 a          347 c          453 g          246 t
ORIGIN      
        1 tgcgacgcgc acgactggtt ggccggattg acgttgggcg gaaacaggaa ggtcgagccg
       61 gagacgcacg cggcgtacga ccccgagacg gtgccgttga cgtaccacga gttaccgttg
      121 agcgcgcgcc cgttcaagcg cacttccacg ccggtatcca aaccgacggc gttgttgaag
      181 acggcgaaga gcggcgtgtc gagcagctgg gtggtgtcga gtacgttcac gaccgtcttg
      241 cgaaagacgt tgatcgagcc cgtgagaccg gaatcgaaga cgtgatccat gcccatctcg
      301 gcgtaggcgt cgcgttcggg ctggaggttg taaaccggtg tcgacgggca cccctgcagt
      361 acgacgcaat cctggcgaac gtcttcgagc agcggtgcgg catagaagcg gccgtaatat
      421 gcgtgaaacg tgttcttgcc gccgtcccag aggttcaggc cgatgcgcgg actgaactgc
      481 cagccgccgg tgtatccggt ggaatggtcg tagcgcaatc cgtagttgat ttgcgcgtat
      541 tttgagagct gccatttgtc ttcggcgaag atacccactt gcgagccggc ttgtgcctgc
      601 tcgggtgacg cgacgagata gtacggctgc gcgtgtacgc cggaaccggc gcagccggcg
      661 tagtaacagc cgaacgcctg ggtagcctcg aggttggagc gatcgatatc gaagccgacc
      721 ttccaggcat ggttttcggt cgcgcggaaa tctgcggcgc gcaaaccgac gtagttcgcg
      781 tacgtgttcg acagcagacc gacgttatgc gccggcagcg atgggtcgcc gaaggtcgcg
      841 caactcgtgc agacgatgcc cgagccgaag ttgggcgtca ggccgaggac gtcgttgggc
      901 aaatcgccgt cgtaattgat gcgggtcgag cgccaccacg gtatgacctg gaacacgccg
      961 ttgccgtcgc gcgacgtttg ggtgtagtta aggttcgcga agcggtcgta ctcgcgctgc
     1021 acgtcgtcgg tgccggctgg cgtgtagatc gggtcgtagg gattgttcgg atctgtgttg
     1081 atcgggattt ggaactgcgc gagctgattg gagtagtcgc cggcgagcgt cgcgcgcggg
     1141 cccagcgcgg tgacgaagcg cgcgaattgg tcgctttgcg acgagttatc gtggatcggg
     1201 tcgaaggtcg gcgcgtccaa gccgcgggca tcgctctggg tgttggcgtt gacgaacagc
     1261 tcgctcgcgc cg
//
LOCUS       DASUCJ010000120         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1315558, whole genome shotgun sequence.
ACCESSION   DASUCJ010000120 DASUCJ010000000
VERSION     DASUCJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315558"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..770)
                     /locus_tag="VFU90_01445"
     CDS             complement(162..770)
                     /locus_tag="VFU90_01445"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5478460.1"
                     /translation="MFTGIVQSTGRIARCEPRGGDLRMIVAVAAGFGTAEVALGDSIA
                     VSGCCLTAIMSDDDTLAFDISNETLSLTTLGGFRPGERVNLEPALRLSDRLGGHLVAG
                     HVDGVGTIAAVESDARSQRWTIEAPPGLMRYFAPKGSVCVDGVSLTVNAVGGNRFGVN
                     LIPHTMAATTFGERRVGDRVNLEVDLLARYVERLLVRPEAAA"
     regulatory      complement(893..1037)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1062..>1272)
                     /locus_tag="VFU90_01450"
     CDS             complement(1062..>1272)
                     /locus_tag="VFU90_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478461.1"
                     /translation="VDELLLYVAPVVLGDRARPLFALPPLATMASRWRLRTLEQRMVG
                     RDLRLLLRPEPDGKDRPRQPAPAAV"
BASE COUNT          210 a          463 c          403 g          196 t
ORIGIN      
        1 caggccgcgc ccttcgcgcg ccatgaagtt gatgtcttct gcgcgcacct tgtcggccgc
       61 catgatcagg tcgccctcgt tctcgcggtc ctcgtcgtcc agcatcacca ccatgcgacc
      121 ggcggcgact tcctccagca attcgggaac ggtcgcgaag gtcatgcggc ggcctccgga
      181 cggaccagca agcgctccac gtaacgcgcc agcaagtcga cctcgaggtt cacgcggtcg
      241 ccgacgcggc gttccccgaa cgtcgtcgcc gccatcgtgt gcggaatcag gttgactccg
      301 aaacggtttc ctccgaccgc gttgaccgtc aggctgacgc catcgacgca gaccgagcct
      361 ttcggcgcga agtagcgcat cagcccgggc ggcgcctcga tggtccagcg ttgcgagcgc
      421 gcgtcggact cgaccgctgc gatcgtgccg acgccatcga cgtgccccgc gaccaggtgc
      481 ccacccagcc ggtcggagag ccgcagcgcc ggttcgagat tcacgcgctc gccaggccga
      541 aatcccccca gcgtcgtcag cgacagcgtt tcattggaaa tgtcgaacgc cagcgtgtcg
      601 tcatcgctca tgatcgcggt gaggcagcac cccgacaccg cgatgctgtc gcccagcgca
      661 acctcggcgg tgccgaatcc ggccgcaacg gcgacgatca tccgcaggtc gcccccacgc
      721 ggctcgcagc gcgcgatccg gcccgtggac tggacgatgc cggtaaacat cagccgcgct
      781 ccgggccggg accggggata ttcgaagatg ggcagtgcat gaacgttctc cgccagacca
      841 tcgcgagaac gccccaaggc gtcaggccga cgcgccgttc gccgacgcgt gcggccgtct
      901 tctttcatcc ggactgtcac cgtcggctcc ggcattcgac cggatctgct gacctttcgc
      961 tgttgcgaaa gcgctcgcgg gcttgcccca ttgggcctac cgccggtggg gaattgcacc
     1021 ccgccctgaa gacgttgccg gaatgatccg gcgcggcaag tttacaccgc cgcgggcgcc
     1081 ggctggcggg gacgatcttt accgtctggt tccgggcgaa ggagcagccg caagtccctg
     1141 cccaccatgc gctgttcgag cgtgcgcaac cgccagcgcg aagccatcgt ggccagcgga
     1201 ggcagcgcga acaacggccg cgcgcggtca ccgagcacga ccggcgccac gtacagcaac
     1261 agctcgtcga cc
//
LOCUS       DASUCJ010000121         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1269851, whole genome shotgun sequence.
ACCESSION   DASUCJ010000121 DASUCJ010000000
VERSION     DASUCJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269851"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1172)
                     /locus_tag="VFU90_01455"
     CDS             complement(66..1172)
                     /locus_tag="VFU90_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5478462.1"
                     /translation="MSARSLIASLLCVIAGACSGGSPTIQLGVAGPFAQPFGDMTKKG
                     IEMAIAEVNDSGGVRGKHLELEERDDAADGAKAAGIAQDFVANQAVVGVIGHVTSGAM
                     VAAAKVYDSGRLPAIATSATTPELTGMSPWVFRVISSDSSNGITIAHFAEALGRKRAA
                     VLYENDAYGRGLASSFRRAFDGEIVSIDPISADITNAEPYIAYYKTRSPDVVFVVGTQ
                     NSGLVVLREAKREKFATDFLGGDGWTGIVADTAMAEGTYVGTAFTVEDPRPEVQRFVS
                     DFRARYHEVPDAYAALGYDAARMMARAVQEAGPDRAAIRAYLASLTDETAYHGITGVL
                     RFGPGGDPVTSSYRVTRVRHGAFELVTPGAGSSR"
BASE COUNT          267 a          526 c          533 g          277 t
ORIGIN      
        1 gaagccgagg aggagacgag cgcgaatcgt cgtcgtgcgc tggagcggtg tgaaggtcgc
       61 ctcgctcatc ggctgctccc ggcgccgggc gtcacgagct cgaacgcgcc atggcgcacg
      121 cgggtcacac gatacgatga cgtgaccggg tcgccgcccg ggccgaagcg caacacaccg
      181 gtgataccat ggtacgcggt ttcgtcggtg agcgacgcga gatacgcccg gatcgccgcg
      241 cggtcgggac cggcctcctg caccgctcgc gccatcatcc gtgccgcgtc gtagcctaac
      301 gcagcgtacg cgtcggggac ttcatggtag cgcgcgcgga aatcggacac gaagcgctgc
      361 acctcggggc gaggatcctc gacggtgaac gcggtgccga cgtacgtgcc ctcggccatc
      421 gccgtgtcgg cgacgatgcc cgtccagccg tcgccgccca gaaaatcggt cgcgaacttc
      481 tcgcgcttcg cttcgcgcag caccacgagc cccgagttct gcgtgccgac cacgaacacc
      541 acgtcggggg atcgcgtctt gtagtacgcg atgtacggtt cggcgttcgt gatgtcggcg
      601 gaaatcggat cgatgctcac gatctccccg tcgaaggcgc gccggaaact cgaggcgagc
      661 ccgcgtccat acgcatcgtt ctcgtacagc accgccgcgc gcttgcgacc cagcgcctcg
      721 gcgaagtgcg cgatcgtgat gccgttgctc gagtcgctgg agatcacgcg gaacacccac
      781 ggcgacattc cggtcaactc gggtgtggtc gccgaagtcg cgatcgccgg cagccgccca
      841 ctgtcgtaga ctttcgccgc ggcgaccatc gcgcccgacg tgacgtgtcc aatcacgccg
      901 acgacggcct ggttcgcgac gaaatcctgc gcgatgccgg ccgctttcgc gccgtcagcg
      961 gcgtcgtcgc gttcctcgag ctcgagatgc ttcccgcgga cgccacccga gtcgttgacc
     1021 tcggcgatgg ccatctcgat gcctttcttg gtcatgtcgc cgaacggctg ggcgaacgga
     1081 ccggcgacgc caagctgaat cgttggtgaa ccgccggagc aggcgccggc gatgacgcag
     1141 agcagcgaag caatgaggga acgcgcgctc acggcgagta tggaaggcgg tgcgggtcta
     1201 gcggtgcgcc aaagcgctca cgcgtcgttg gcagcgcccg ctgaatgaga tgactttcac
     1261 ggagatggct tcggtggcgc agagcgtact ctcgggacga agcgctgacc gacgtgtaac
     1321 agagcgctcc actgatcggg cgcctgaacg aaggtctttg tcgtccgcga cctcactgcc
     1381 ctgtgcagcg gcgcgtccgg cgccagctag gtcaggccta aggcgcgtcc gaatcgccct
     1441 gtcggtagat tcctgcagat tcggcgcgta cggcgataca catcacggtc gtttggccgt
     1501 ctgtgtaccg tctttatgct cctgcgtgct gcattattgg caggatttca cgtacagcgg
     1561 aggttatcat gagtggccca cgtaggcggt cctgctctcg acc
//
LOCUS       DASUCJ010000122        15587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1147452, whole genome shotgun sequence.
ACCESSION   DASUCJ010000122 DASUCJ010000000
VERSION     DASUCJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 15587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15587
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147452"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFU90_01460"
     CDS             complement(<1..523)
                     /locus_tag="VFU90_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5478463.1"
                     /translation="MYIVVTGAAGFIGSNLVKALNAEGETDIIAVDDLTDSTKFANLV
                     DCDIADYLDKDEFLERLADGDFDDDISTVFHQGACSDTMERDGRFMMRNNYRYSATLL
                     EYCQDNDIAFLYASSAAVYGAGTVFREERAHEVPLNVYGYSKRLFDDHVRRVLPERTA
                     QIAGFRYFNVYGPR"
     gene            complement(539..1618)
                     /gene="alr"
                     /locus_tag="VFU90_01465"
     CDS             complement(539..1618)
                     /gene="alr"
                     /locus_tag="VFU90_01465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5478464.1"
                     /translation="MTRPLVAQVDLAALKANVARVRQYAPAAKVLAVVKANAYGHGLE
                     RVLPALDDADGLALLELDAALRLRGCGYARPLVLLEGCFDARELAEVAGAGIEIVVHS
                     PWQTAMLREAKLSRRVGVHVKINTGMNRLGFAPADARGACDALAALPAVDVRTIMTHF
                     ASADEERGLDAPLARFAEACRGSRLPRSLANSAGVVRYAEVGGDCVRPGIMLYGATPF
                     ADRSAASLGLVPVMTLRTRLIGVQSLAAGESVGYGGMFTTHEPARIGIVACGYADGYP
                     RHAPSGTPVLVAGRRAVTAGRVSMDMLAVDLTGIPDADVGSDVVLWGEGLPVDEVAAA
                     AGTVGYELLCAVAPRVAFETVAIAA"
     gene            1729..3009
                     /gene="lplT"
                     /locus_tag="VFU90_01470"
     CDS             1729..3009
                     /gene="lplT"
                     /locus_tag="VFU90_01470"
                     /inference="COORDINATES: protein motif:HMM:NF008397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEU5478465.1"
                     /translation="MKRGFFTIIAAQFFSSLADNALLIAAIALLRNLDQPHWMTPALK
                     QCFVVSYVVFAPLVGAFADSMAKGRVMLITNAVKFAGCALMLFYVHPLLSYALVGLGA
                     AAYSPAKYGILTELLPPEKLVIANGWMEGTTVASIILGVLLGGALITPRVSTRLLGID
                     LPLIDLPIDTAPQAAIAVIMSLYLLAAIVNWFIPDTGVDHRAPSVNPLYLVREFSHCV
                     GLLWRDRLGQITLATTTLFWGAAATMQFIVIDWSATSLALSLSQSTFLQGAVLVGAAI
                     GTMLAAGLVSLRRSVAVLPVGVAVGVILMVIVLVTKLSVAVVLLIAIGACTGFFVVPM
                     NALLQHRGHVLMGAGHSIAVQNFNENLGILVTVGMYALMLRAGLPINVAIMLFGAFIS
                     VAMLLVIWRHRYNESEHDSMRLIGVDKPGTATSD"
     gene            complement(3054..3563)
                     /locus_tag="VFU90_01475"
     CDS             complement(3054..3563)
                     /locus_tag="VFU90_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5478466.1"
                     /translation="MTHRSLTRRLVAHLATDHLSVRRAFPRSALARIEEAIAAGERKH
                     RAQICFAVEAALHPAHLLRRLTPRERALEVFGLLRVWDTEENAGVLIYLLLADRDIEI
                     VADRGAARAAGPHAFAPIAAQMEEAFASRRFVDGVVAGIGEISTLLAQHFPRLHDGPN
                     ELPDRAVVL"
     gene            complement(3567..4532)
                     /locus_tag="VFU90_01480"
     CDS             complement(3567..4532)
                     /locus_tag="VFU90_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5478467.1"
                     /translation="MQEPRGSGGVATATLTARIIAWLAMFAALAVPALAVAQPASLAG
                     SAAPAPVPTLTQRVTDLTGTLSGSERQALDARLADWERASGNQLVVLLVPTTRPEPIE
                     AYSIRVAEAWKIGQKGRDNGAIFLIAKNDHRMRIEVGYGLEGVLTDITSHRIIDETVT
                     PLFREGKFAEGINAGVDRIIAVVNSGNPLPPARAAPPARGKGGVDLQSLLVLLLVVVP
                     VVGSVLSRIFGRALGSTVGGGVIGLGAWLIAGSIAIGVGAGVIAFLVMLLFGAAGGIG
                     RSGGMWVPMGGGFGGGGFGGGGFGSGGGFSGGGGGFGGGGASGSW"
     gene            complement(4561..5235)
                     /locus_tag="VFU90_01485"
     CDS             complement(4561..5235)
                     /locus_tag="VFU90_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5478468.1"
                     /translation="MLRKLATLLAVIATLSLSGCGYNTLQTQDEAIKAAWSEVLNQYQ
                     RRADLVPNLVNTVKGYAAQEQTVLTEVTNARATVGSIKATPELVNDPVAFQKFIAAQN
                     QLQGALSRLLAVSENYPQLKSDALFRDLLSQLEGTENRIAVARNRYIKAVQEYNTTVR
                     RFPTNLTAMVFKMGTKANFTVTDEAAVSTPPKVDFAKPAPAVPAPSTPAPMAPAAPTP
                     AAPASK"
     gene            complement(5343..6074)
                     /locus_tag="VFU90_01490"
     CDS             complement(5343..6074)
                     /locus_tag="VFU90_01490"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5478469.1"
                     /translation="MLREMGLALLWRLRRAGAPAQPDRSAADVVAADVVATDAVATDA
                     VAADAREARIAALDFDAIAADIAACTACPLCRTRHRTVPGIGDPHARWLFVGEAPGAD
                     EDARGEPFVGRAGRLLDNMLAALGLARGRDVYIANVLKCRPPGNRTPAPLEVAACRPY
                     LDRQIELIAPTLIVALGKVAAAALLGTDQPVGAMRGRVHRYRGTALIVTYHPAYLLRS
                     ATEKARAWEDLLFARRKLREIAAAD"
     gene            complement(6097..6654)
                     /gene="rimI"
                     /locus_tag="VFU90_01495"
     CDS             complement(6097..6654)
                     /gene="rimI"
                     /locus_tag="VFU90_01495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5478470.1"
                     /translation="MATLLSNVTRAATLARVAWRPLTAADVETVAALEARIHAAPWSV
                     GNFHDALRAGYAARAGLRVGRIVAYGVLMLGPGEAQLLNLSVAPEARREGLGRALLAQ
                     FIETAEHFAAEQMFLEVRVSNLAAISLYERQGFVAVARRASYYPPAPSGEREDALVMR
                     CALGRLRRSLAGPAEPIGGAGGESG"
     gene            complement(6663..7373)
                     /gene="tsaB"
                     /locus_tag="VFU90_01500"
     CDS             complement(6663..7373)
                     /gene="tsaB"
                     /locus_tag="VFU90_01500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5478471.1"
                     /translation="MKLLAIECSTAWLSVCAHDGRALATRRERSGASASARILGFVEE
                     VLADVGWRLGELDGIAFGCGPGAFTGLRVACGTVQGLALGASLTVVGIASLAAMAQAA
                     WRIHGATRVVGCLDARMRQVYLGAYQRGVDLSVNDWLTDVDPIVCPPGEITPPRSGTS
                     GWFGAGDGFTVDPGLVQRLALAGVDASIVPDALDVATLAEQSFAAGGGRPARDAQPLY
                     VRHRVALTEAERVAGVRL"
     gene            7430..8782
                     /gene="rlmD"
                     /locus_tag="VFU90_01505"
     CDS             7430..8782
                     /gene="rlmD"
                     /locus_tag="VFU90_01505"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5478472.1"
                     /translation="MLELTVESLDQDGRGVARAEGKVVFVDGALPREVVEAETLRRKP
                     SFDVARTVSVRSANPGRCEPRCPHFGVCGGCTLQHADLPLQVAAKQRVLEETLARIGG
                     VRPATILPAIAGPAWGYRDRARLSVRDVPKKGGVLVGFHERRSSYVADMRVCHVLPPR
                     LSELLLPLRALVESLSIRTRLPQIEVAVGERLVPRSAAGAGTDAAQRIYALVLRILAP
                     PSDVDLARIVAFADTHGVEFWLQTGGPETVVPFHPPSATLAYTLPEFDVALPFTPTDF
                     TQVNTAVNRVLVRRALQLLAPQPRERVGDFFCGLGNFTLPIARRGAFATGFEASRTLV
                     ARALENARRNALGASTAFVCADLFRSAPDAIARAGPLDRILLDPPREGAIELVKALPA
                     HDAGEAPQRIVYVSCNPATLARDAAVLVHQRGYVLDAAGIVNMFPHTAHVESIALFTR
                     "
     gene            complement(8915..9628)
                     /locus_tag="VFU90_01510"
     CDS             complement(8915..9628)
                     /locus_tag="VFU90_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5478473.1"
                     /translation="MDPRLQPVTIDQSTAQVGQVTADAQHRVLRNTYWLLALSMLPTI
                     AGAWTGMQFNLVKLFVAAPVMTPLLMFAVMIGALFAVTALRNSAWGVPALFGFTFIAG
                     LMLAPILSVAAGFRNGGQLVALAGGMTAAIFFGMATLATVSKRDFSFLGKFLFVGLML
                     LIVASLANLFFQVPAVTLTVSAIAVLIFSAYLLYDVSSIVRGGETNYISATLKLFLDI
                     YNIFISLLNLLLAFSGQRD"
     gene            9746..9835
                     /locus_tag="VFU90_01515"
     tRNA            9746..9835
                     /locus_tag="VFU90_01515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9780..9782,aa:Ser,seq:tga)
     gene            9999..11234
                     /locus_tag="VFU90_01520"
     CDS             9999..11234
                     /locus_tag="VFU90_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5478474.1"
                     /translation="MTKKALTDTAVRNAKYNARGPKKQTLSDSRGLYVELAPTGGKWW
                     RLAYRFGGKRKTISLGTYPTVSVADARNLRDDAKKLLAKGIDPSAQRQTEKREVAGRA
                     ANSFEAVAREWYDKQAHRWVPHHASDVLRRMESNLFPELGDKPISAITAPMLLSAVRK
                     IEHRGAHDLAHRVLQVASQIFRYGVASGRCERDPAPDLRGALTPHKKRNQAAVTIDEL
                     PKLLRDIAEYHPKVGDKLTGLALQLLAMTFVRTGELIGATWDEIDLKNAVWIIPAARM
                     KMRSEHVVPLSRQADALLRGVQDIGGGSRYVFPGRNPDKPMSNNTMLFALHRLGYKGK
                     MTGHGFRAVASTILNESGRYRSDVIERQLAHCEHNDVRGAYNRAAYLPERRRMMQDWS
                     DTLDALVKGAQVIPLQRRA"
     gene            11530..11799
                     /locus_tag="VFU90_01525"
     CDS             11530..11799
                     /locus_tag="VFU90_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEU5478475.1"
                     /translation="MPTTTTLIRLADVMRIVGFGKSQIYKLIGRGEFPLPVKLGRASR
                     WSASEVAEWVQGRIATRSPGAWGPMSPNRIAPRREVLYTDGGTRG"
     gene            11792..12373
                     /locus_tag="VFU90_01530"
     CDS             11792..12373
                     /locus_tag="VFU90_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478476.1"
                     /translation="MGRSRGKSRTEPFTAIPRDVLNSHAFKSLPAIYRALYLDLRRQA
                     TPWQNGKIAASDYTLGVFGWSHSTIHKGLALLIEHGLLERTRRGGLGDRGKKRSLYGF
                     TDMAIEADEGRGIRGRLPTYAYRNYKPAKEVSEDRPQEIKQRPRSEPFRSIRCTSKAD
                     TVHAVDSKETQGPPNGPLYEPVTIPVFREQIAA"
     gene            12917..13120
                     /locus_tag="VFU90_01535"
     CDS             12917..13120
                     /locus_tag="VFU90_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478477.1"
                     /translation="MTEPADTTPSGASPSPQTGEADADGSNPAGDAVESGDKWDALLK
                     RIAEREKRYTPAKREMARAFGFL"
     gene            13736..15361
                     /locus_tag="VFU90_01540"
     CDS             13736..15361
                     /locus_tag="VFU90_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEU5478478.1"
                     /translation="MQNALYFGDNLDVLRESIKDESIDLIYLDPPFNSNATYNVLFKT
                     PKGHQSDAQIEAFDDTWHWGPHAEAAFAELMQQSNTDVAEVTHALRSVLGENDMMAYL
                     VMMGIRLLELHRVLKPTGSLYLHCDPTASHYLKIVLDQVFSPEFFRNEIVWQRTNVHS
                     DSKTWSRVSDSILFYTRSNAFTWNPIYLPHSDEHIEAKYRSADPDGRRYTLSDMTSPA
                     PRPNMMYVWRGFPTPNFGWRYSKETVEKLDAEGRIWYPTFKNGQRDTSKRPRLKRYLD
                     EMPGTLLTNVWTDIYPINSQAQERLGYPTQKPLALLERIIVASSNEDDLVLDPFCGCG
                     TAVDAAEKLKRRWVGIDVTHLAVSLIEKRMRDRYPKIEFVVHGTPKDLDGARDLATRD
                     KYQFQWWACSLVNAQPFQGRKKGADSGIDGIIYFQDDDKLPKKIVVSVKGGDNVNVAM
                     VRDFAHVIEREKAAMGFFVTLTDPTKNMVTESVGTGYYTSPLTGAKYPKLQVLTIEGL
                     LSGQERPAYPRMDAGGLTFKKAKEAAPEQKQLL"
BASE COUNT         2826 a         5200 c         5076 g         2485 t
ORIGIN      
        1 cgcgcggacc gtagacgttg aagtagcgga agcccgcgat ctgcgcggtg cgctcgggaa
       61 gcacgcgccg cacgtgatca tcgaacaggc gcttcgagta tccgtacacg ttgagcggca
      121 cctcgtgcgc acgctcttcg cggaacacgg tgccggcgcc gtagaccgcg gcgctcgatg
      181 cgtacaggaa cgcgatgtcg ttgtcctggc agtactcgag cagcgtcgcc gaatagcggt
      241 aattgttgcg catcatgaag cggccgtcgc gctccatcgt gtccgagcac gcgccctggt
      301 ggaagaccgt cgagatgtcg tcgtcgaaat caccgtcggc aaggcgctcg aggaattcgt
      361 ccttgtcgag atagtcggcg atgtcgcagt cgacgaggtt cgcgaacttg gtggaatcgg
      421 tgaggtcgtc gaccgcgatg atgtcggtct caccctcggc gttcagcgcc ttgacgagat
      481 tcgatccgat gaatccggcg gcgccggtga ccacgatgta cacgttcgat tctcccggtc
      541 aggccgcgat cgcgactgtc tcgaatgcga cgcgcggcgc caccgcgcag aggagctcgt
      601 agccgacggt gccggccgcc gcggcgacct cgtcgacggg aagcccttcg ccccacagca
      661 cgacgtcgct gccgacgtcc gcgtcaggaa tgccggtaag gtcgacggcg agcatgtcca
      721 tcgagacacg cccggcggtg accgcgcggc gcccggcgac cagcaccgga gtaccgctcg
      781 gcgcgtggcg cgggtaaccg tccgcatagc cgcaggcgac gatgccgatc cgtgccggct
      841 cgtgcgtcgt gaacatgccg ccgtaaccga cgctttcccc ggcggcgagc gactgcacgc
      901 cgatcagccg cgtacgcagt gtcatcaccg gtaccagccc gagcgacgcg gcgctgcggt
      961 ccgcgaacgg ggtcgcgcca tagagcatga tccccggccg cacgcagtcg ccgccgacct
     1021 cggcgtaacg gaccaccccg gccgaatttg cgagcgaacg cggcaagcgc gagccgcggc
     1081 aggcctccgc gaagcgcgcg agcggtgcgt cgaggccgcg ctcctcgtcg gcgctggcga
     1141 agtgcgtcat gatggtcctg acgtcgaccg caggaagcgc cgcgagcgcg tcgcacgccc
     1201 cacgcgcgtc ggcgggggcg aagccgaggc gattcatccc ggtattgatc ttgacgtgca
     1261 caccgacgcg ccgcgagagc ttcgcttcac gcagcatcgc agtctgccat ggcgaatgca
     1321 cgacgatctc gatgccggcg ccggcgacct cggccaactc gcgggcgtcg aagcatcctt
     1381 cgagaaggac gagcggccgc gcatagccgc acccgcgcag ccgcagcgcg gcatcgagct
     1441 cgagcagcgc gagtccgtcg gcatcgtcga gcgccggaag cacgcgctcc agaccgtgcc
     1501 cgtatgcgtt cgccttgacg accgccagca ccttcgccgc gggcgcatac tgccggacgc
     1561 gggcgacgtt tgccttcagc gcggcgaggt cgacctgcgc gaccagcggg cgagtcatgc
     1621 ggacgcagtc atggtataaa gcgcgggccg cgcgccgtgc tcgcattata gacgccatgt
     1681 ccctccggcg aacccgcagc cgatgccggg tgccgaccca tcgagttcat gaaacgcggc
     1741 ttcttcacca tcattgcggc gcagttcttc tcgtcgctcg ccgacaatgc gctgctcatc
     1801 gccgccatcg cgctgctgcg caatctggac cagccgcact ggatgacgcc ggcgctgaag
     1861 caatgcttcg tggtgtcgta cgtcgtgttc gccccgctcg tcggcgcctt cgccgattcg
     1921 atggcgaaag gacgcgtcat gctgatcacc aacgcggtga agttcgccgg ctgcgcgctg
     1981 atgctgttct acgtccaccc gctcctgtcg tacgcgctgg tcggtcttgg cgccgccgcc
     2041 tattcacccg ccaagtacgg catcctgacc gagctgctgc cgccggaaaa gctggtcatc
     2101 gccaacggat ggatggaggg cacgacggtc gcgtcgatca tcctcggcgt gctgctgggt
     2161 ggcgcgctga ttactccgcg cgtctcgacg cggctcctcg gcatcgatct tccgctgatc
     2221 gacctgccga tcgacacggc accgcaggcg gcgatcgcag tcatcatgag cctctatctg
     2281 ctcgccgcga tcgtcaactg gttcatcccc gacaccggag tcgaccaccg tgcgccgagc
     2341 gtcaatccgc tctacctcgt gcgcgagttc tcgcactgcg tagggctgct gtggcgcgac
     2401 cgcctcggtc agatcacgct cgccacgacg acgctgttct ggggtgctgc agcgacgatg
     2461 cagttcatcg tcatcgactg gtcggcaacc tcgctcgcgc tcagcctgtc gcagtccacc
     2521 ttcctgcagg gtgcggtgct ggtgggtgcg gcgatcggca cgatgctcgc cgccgggctc
     2581 gtcagcctgc gtcgatcggt cgccgtgctg ccggtcggcg tcgccgttgg ggtgatcctg
     2641 atggtcatcg tcctcgtgac gaagctctcg gtcgcggtcg tgctgctcat cgcgatcggc
     2701 gcgtgcacgg gattcttcgt cgtcccgatg aacgcgctcc tgcaacatcg tggccacgtg
     2761 ctgatgggcg ccggccactc gatcgccgtg cagaacttca acgagaacct cggcatcctc
     2821 gtcaccgtag gcatgtacgc gctgatgctg cgggccggcc tgccgatcaa cgtcgcgatc
     2881 atgctcttcg gggcgttcat ctccgtcgcg atgctgcttg tgatctggcg ccaccgctac
     2941 aacgagtccg agcacgactc gatgcgcctg atcggcgtcg acaagccggg caccgccacg
     3001 tcggactgac cgccagaagc gcgaaccgac cggctacagc atggagtgac cggctacagc
     3061 acgacggcgc ggtcggggag ctcgttcggc ccgtcgtgga ggcgcgggaa gtgctgtgcg
     3121 agcagcgtcg agatctcgcc gatgcccgcc acgacgccgt cgacgaagcg tcgacttgca
     3181 aacgcttcct ccatctgggc cgcgatcggc gcgaacgcgt gcgggcccgc ggcgcgcgcg
     3241 gcgccgcggt cggcgacgat ctcgatatcg cgatcggcga gcagcaggta gatcagcaca
     3301 ccggcgtttt cctcagtgtc ccatacgcgc agcagcccga acacctcgag cgcgcgttcg
     3361 cgcggcgtga gccggcgcag caggtgggcc ggatgcaacg ccgcctcgac cgcgaagcag
     3421 atctgcgcgc gatgcttgcg ctcgcctgcg gcgatcgctt cctcgatacg ggcgagcgcc
     3481 gatctcggga acgcacgccg cacagacagg tgatcggtcg cgagatgcgc gacaagccgc
     3541 cgggtcagcg agcgatgggt catctgctac cagctccccg atgcgccgcc gccgccgaag
     3601 ccgccaccgc cacccgagaa gccacccccg ctgccgaacc ctccaccacc gaaaccgcca
     3661 ccgccaaagc cgcctcccat cgggacccac atccctccgc tgcgcccgat gccgcccgcc
     3721 gcaccgaaca gcagcatgac caagaatgcg atcacgccgg cgccgacgcc gatcgcgatc
     3781 gagcccgcga tcagccacgc accgagtccg atgacaccgc cgccgacggt cgacccgagc
     3841 gcgcgcccga agattcgcga caggacggag ccgaccactg gcaccacgac gagaagcagc
     3901 acgagaagcg actgcaggtc gacgccgccc ttgccgcgcg cgggaggcgc ggcccgcgcc
     3961 ggcggcagcg ggttcccgga attcacgacg gcaatgatgc ggtcgacgcc ggcgttgatg
     4021 ccttcggcga acttgccttc gcgaaacagc ggcgtaaccg tttcgtcgat gatccgatgc
     4081 gaggtgatgt cggtgagcac accctcgagg ccgtagccga cctcgatgcg catgcggtga
     4141 tcgttcttcg cgatgaggaa gatcgccccg ttgtcacgcc ccttctgccc gatcttccac
     4201 gcctccgcga cacgaatcga atacgcctcg atcggctccg gcctggtcgt cggcacgagc
     4261 agcaccacga gctgattgcc gctcgcacgc tcccagtcgg cgagcctggc atcgagcgcc
     4321 tgccgctcgc tgcccgacag ggtgccggtc aggtcggtga cgcgctgggt cagcgtgggc
     4381 accggcgccg gcgcggccga tcccgcaagc gacgccggct gtgccacggc gagtgccgga
     4441 acggcgagcg ccgcgaacat cgcgagccac gcgatgatcc gcgccgtcag cgtcgcggtc
     4501 gccacgccac ccgatccgcg gggctcttgc atcggctcga gcgcaacgcg caacgccgcc
     4561 ttacttgctc gccggcgctg ccggagtcgg cgcggcagga gccatcggcg ccggcgtcga
     4621 cggcgcgggt acggccggcg caggcttcgc gaaatcgacc ttgggcggcg tgctcaccgc
     4681 ggcctcgtcg gtcaccgtga agttcgcctt ggtgcccatc ttgaatacca tcgctgtcag
     4741 gttggtcggg aaccgccgga ccgtcgtgtt gtactcctgc accgccttga tgtagcgatt
     4801 gcgcgcgacg gcgatccggt tctcggtgcc ttcgagctgc gacagaaggt cgcggaacag
     4861 cgcgtcggat ttgagctgcg ggtagttctc ggataccgcg agcagccgcg acagcgcgcc
     4921 ctgaagctgg ttctgggcag cgatgaactt ctggaatgcg actggatcgt tgacgagctc
     4981 gggcgtcgcc ttgatcgatc ctacggtcgc gcgcgcgttg gtgacctcgg tcaggacggt
     5041 ctgttcctgt gcggcatagc ccttgaccgt gttgacgagg ttcggaacca gatccgcgcg
     5101 ccgctggtac tggttcagca cctccgacca ggcggccttg atcgcttcgt cctgtgtctg
     5161 cagcgtgttg tagccgcatc ccgaaagtga cagcgtcgcg atgaccgcga gcagcgtggc
     5221 gagcttgcgc aacatcgatt cccccaagag gacgccggtc gatctgcccg gcccaatcgc
     5281 aatatgcggt ctcggggccg gcggcgcaag agcggtgccc acatcgcggc ggcgccttgc
     5341 cgtcaatccg ccgcggcgat ctcgcgcagc ttgcggcgcg cgaacaggag atcctcccaa
     5401 gcccgagctt tctcggtggc gctgcgcagc agataggcgg gatggtaggt cacgataagc
     5461 gcggtgccgc ggtaacgatg cacccgcccg cgcatcgcgc cgaccggctg gtcggtgccg
     5521 agcagtgccg cggcggcgac cttgccgagc gcgacgatca gcgtcggcgc gatcagctcg
     5581 atctgccggt cgaggtacgg ccggcaggca gcgacttcga gcggcgcggg tgtgcgattg
     5641 ccgggcgggc ggcacttcag cacgttcgcg atgtagacgt cgcggccccg ggcgaggccg
     5701 agcgcggcga gcatgttgtc gagaagccga ccggcccgcc cgacgaaggg ctcgccgcga
     5761 gcgtcctcgt cggcgcccgg agcttcgccg acgaacagcc agcgcgcatg cgggtcccca
     5821 atccccggca ctgtgcgatg gcgcgttcgg cacagtggac acgctgtgca cgcggcgatg
     5881 tcggcggcta tcgcgtcgaa atcgagcgcc gcaatgcgcg cctcccgcgc atcggccgcg
     5941 acggcatcgg tcgcgacggc gtcggtcgca acgacatcgg ccgcaacgac atcggccgcg
     6001 ctccgatctg gttgcgccgg cgcgccagcg cgccgcaacc gccacagcaa tgcgaggccc
     6061 atctcgcgaa gcaattccga acgttccagc actgactcag cctgattcgc cgccggcgcc
     6121 gccgatcggc tcggcaggcc cggcgagcga tcgtcgcagg cgcccgagcg cgcagcgcat
     6181 caccagcgcg tcctcgcgct cgccactcgg tgccggcgga tagtagctcg cccgtcgcgc
     6241 aacggcgacg aacccctggc gctcgtacag ggaaatcgcc gccaggttgc tcacgcgcac
     6301 ttcgaggaac atctgctcgg cagcgaaatg ctcggccgtc tcgatgaact gcgcgagcag
     6361 cgcgcgtccg agcccttcgc ggcgcgcttc cggcgcgacc gacaagttga gcaactgcgc
     6421 ctcgccgggg ccgagcatca acactccata ggcgacgatc cgcccgacgc gcagacccgc
     6481 gcgcgccgca tagccggcgc gcagcgcgtc gtggaagttg ccgacgctcc acggtgcggc
     6541 gtggatgcgt gcctcgagcg cagcgacggt ctccacatcc gccgcggtga gcggccgcca
     6601 ggcaacgcgc gcgagggtgg ccgcgcgggt gacattggac agcagcgtcg ccatgattcc
     6661 acctacagcc ggacgccagc gacgcgctcg gcttcggtaa gcgcgacgcg atggcggacg
     6721 tagagcggct gcgcgtcgcg cgccggccgg ccaccacctg cggcgaagct ctgctcggcg
     6781 agcgtggcga cgtcgagcgc gtcgggaacg atcgacgcat cgacaccagc gagtgcgaga
     6841 cgctgcacga gcccgggatc gacggtgaat ccgtcgccgg cgccgaacca gcccgacgtg
     6901 cccgaccgcg gcggcgtgat ctcgccgggc ggacacacga tcggatcgac gtcggtcagc
     6961 caatcattca cactgaggtc aacaccgcgc tggtacgctc cgagatagac ttggcgcatc
     7021 cgcgcgtcga ggcacccgac gacgcgcgtc gcgccgtgga tgcgccacgc cgcctgcgcc
     7081 atcgccgcga ggctcgcgat cccgacgacc gtaagtgatg cgccgagtgc caggccctgc
     7141 acggtgccgc aggcgacgcg caacccggtg aaggctcccg ggccgcagcc gaacgcgatc
     7201 ccgtcgagct caccgagcct ccagcccacg tcggcgagca cctcctcgac gaacccgaga
     7261 atccgcgcgg acgcgctcgc ccccgaacgc tcgcgccgcg tcgcgagcgc gcggccatcg
     7321 tgcgcgcaca ccgaaagcca cgcggtcgag cactcgatgg cgagcagttt catgcgggca
     7381 ttctaccgac agccgcgacg cctcgcgcgt acaatgcgcg ccgtccatca tgctcgaact
     7441 gaccgtcgaa tcgctcgacc aggacggccg cggcgtcgcg cgcgccgagg gcaaggtggt
     7501 gttcgtcgat ggtgcgcttc cgcgcgaagt cgtcgaagcc gaaacgctgc gccgcaagcc
     7561 gagcttcgac gtcgcgcgca cggtcagcgt gcgctcggcc aatcctggcc gctgcgagcc
     7621 gcgctgcccg catttcggag tgtgtggcgg ctgcacgctg cagcacgccg acctgccgct
     7681 gcaggtggcg gcgaagcaac gcgtcctcga ggaaacgctt gcccgcatcg gtggcgtgcg
     7741 gccggcgacg atccttcctg cgatcgcagg cccggcctgg ggttaccgtg atcgggcgcg
     7801 gctttcggtg cgcgacgttc cgaagaaggg cggcgtgctg gtcggcttcc acgagcggcg
     7861 atcgagctac gttgccgaca tgcgtgtatg ccatgtgctg ccgccgcggc tctcggaact
     7921 ccttctgccg ctgcgtgcgc tggtggaatc gctgtcgatc aggacgcgcc tgccgcagat
     7981 cgaggtcgcc gtcggcgagc ggctcgttcc gcgcagcgcg gcgggcgcag ggaccgatgc
     8041 ggcgcaacgc atatacgcgc tggtgctgcg catcctcgcg ccgccgtcgg acgtcgatct
     8101 cgcgcgaatc gtcgcgttcg ccgacaccca tggcgtcgag ttctggctgc agaccggggg
     8161 acccgaaacg gtcgttccgt ttcacccacc gtcggcgacg ctcgcctaca cgctccccga
     8221 gttcgacgtc gcgctgccct tcacgccgac cgatttcacg caggtcaaca ctgccgtcaa
     8281 ccgcgtgctc gtgcggcgcg cgctgcagct cctcgcgccg cagccgcgcg agcgcgtcgg
     8341 cgatttcttc tgcggccttg gcaacttcac gctgccgatc gcccgccgcg gtgcctttgc
     8401 gacgggcttc gaggctagcc gcacgctcgt cgctcgtgcg ctcgagaacg cgcgccgcaa
     8461 cgcgctcggc gcgtcgacgg cattcgtctg cgccgatctc tttcgttccg cgcccgacgc
     8521 catcgcgcgc gctgggccgc tcgaccggat attgctcgac ccgccccgcg agggtgcgat
     8581 cgagctcgtc aaggcgctgc ccgcgcacga cgccggcgag gcgccgcagc gcattgtcta
     8641 cgtgtcgtgc aatccggcga cactcgcgcg cgacgctgcg gtgctcgtcc accagcgcgg
     8701 ctacgttctc gacgcggccg ggatcgtcaa catgttcccg cacaccgcgc acgtcgaatc
     8761 gatcgcgctc ttcacacgtt gagctcgcgc tgtccgcgag ccgagctcga actcttcacg
     8821 cgctgagtac gctcttcacg tgccgagggg ccgccggaaa cagcgacggg gcggaaggcc
     8881 cgccccgtcg aatggcgcct gcctgctggt gcggtcagtc gcgctgaccg gagaacgcca
     8941 gcagcaggtt gagcaggctg atgaagatgt tgtagatgtc gaggaacagc ttcagcgtcg
     9001 ccgagatgta gttcgtctcg ccgccgcgca cgatgctgct cacgtcgtag agcagatacg
     9061 ccgagaagat cagcaccgca atcgccgata cggtgagcgt caccgcgggg acctggaaga
     9121 acaggttcgc gagcgacgcg acgatcagca gcatcagccc gacgaacagg aacttcccga
     9181 ggaacgagaa gtcgcgcttg ctcaccgtgg cgagcgtcgc catgccgaag aagatcgccg
     9241 ccgtcatccc gcctgcgagt gctacgagct ggccgccatt gcgaaagccc gcggcgaccg
     9301 acaggatcgg agcgagcatc agcccggcga tgaacgtgaa gccaaacagc gccggtacac
     9361 cccacgcact gttgcgcagc gcagttaccg cgaacaacgc cccgatcatc actgcgaaca
     9421 tcaggagcgg cgtcatcacg ggcgcggcga cgaacagctt gacgaggttg aactgcatgc
     9481 cggtccacgc cccggcaatc gtcggcagca tcgacaacgc gagcagccaa taggtattgc
     9541 gcagcacacg gtgctgcgca tcggcggtaa cctgcccgac ctgcgccgtc gactgatcga
     9601 tcgttaccgg ttgcaatctc ggatccatgt atcctccgtg taaggggtgc ccctaacggc
     9661 acccgacgcc aaacaaggcc tgaaccagca ttagactagc agaatcgggg acggttctgg
     9721 cgctgcatgc gcggtccgca cccgaggatg ggtggcagag cggttgaatg caccggactt
     9781 gaaatccggc gatcgtgcaa gcgatccgtg ggttcgaatc ccaccccatc cgccagtctc
     9841 acgtccatcg atgtccccgg tagttcgatt cgagttcgca ccgcataggg gaatcgttcc
     9901 acgatcgtcc actccggtct ccaatcgtgc tttgacatcc gccggtaact ggcggtaact
     9961 ttggcggcat ctaccgcctt ctacggagag ttaccgccat gacgaaaaag gcacttaccg
    10021 ataccgcggt tcgcaacgcc aagtacaacg cccgcggccc caagaagcaa acgttatcgg
    10081 attcccgtgg gctatacgtt gagctcgcac cgacgggagg caagtggtgg cggctcgcgt
    10141 accgcttcgg cggcaagcga aagacgatct cgctgggtac gtacccaacg gtctcagtcg
    10201 cggacgcgcg caacctgcgc gatgacgcaa agaagctgtt ggcgaaggga atcgatccga
    10261 gcgcgcagcg tcagaccgag aagcgcgagg tggccgggag agcggcaaac agcttcgagg
    10321 cggtcgcgcg ggagtggtat gacaagcagg cgcatcgctg ggtaccgcac catgcgtccg
    10381 acgtactgcg gcggatggag tccaacttgt ttccggaact cggtgacaag ccaatctccg
    10441 cgattacggc gcccatgctt ctttcggcgg tccgcaaaat cgaacaccgc ggcgcacacg
    10501 accttgcgca tcgcgtacta caggtggcat cccagatatt ccgatacggc gtggcaagcg
    10561 gacgctgcga gcgcgatccc gcgcccgacc tccggggtgc cctgactccc cacaagaagc
    10621 gcaatcaggc ggcggtcacg atcgacgagt tacccaagct gctgcgcgac atcgcggagt
    10681 atcacccgaa ggtcggcgac aaactgaccg ggcttgcgct gcaactcctt gcgatgacgt
    10741 tcgttcgtac gggcgaattg atcggcgcaa catgggacga aatcgacttg aagaatgcgg
    10801 tatggatcat tcctgccgct cggatgaaga tgcgcagcga gcatgtcgtc ccgctctccc
    10861 ggcaggccga tgcgttgctg cgcggggtac aggacatcgg cggcggcagc cgctacgtgt
    10921 tcccgggccg caatcctgac aagcccatga gcaacaacac gatgctgttc gcgctacatc
    10981 ggctgggcta caagggcaag atgacagggc acgggttccg cgccgtcgcg tctacgatcc
    11041 tcaacgaatc cggccgctat cggtccgacg tgatcgagcg gcagttggcc cattgcgagc
    11101 acaacgacgt acggggggcc tataaccgcg ccgcgtacct ccctgaacgg cgccgaatga
    11161 tgcaggactg gtcggacacc cttgatgcgt tggtgaaggg agcgcaagtc attcccctcc
    11221 agcggcgagc gtgagttagc gcggcgcgca gcagcggtgc cctcgggggc gcgcttccgg
    11281 acccgagccg acggacggct gcaaaacggt ccccattcgt ccgtgctcgt ccagatttcg
    11341 ggtggcagcc cgtgcgcgtg atccatatta tgtgaatcgg agtgcggccc tgccaaggtc
    11401 cgatgcttgc gcggtcccgg cgttatcgag taaccgaccg ggagaccgcg agcgcaacac
    11461 ggcggcagga caccgcgcct cggcgcaagc cgaactcatc cgttacaggc actatgcgga
    11521 ggctcggcca tgccgactac cacgacactc attcgcctcg ccgacgtaat gcgcatcgtc
    11581 gggtttggca agtcgcaaat ctacaagctg atcggcaggg gtgaattccc gcttcctgtg
    11641 aagctcggtc gtgcgtctcg atggtccgcc tcggaagtcg ccgagtgggt acaaggaagg
    11701 atcgccacga ggagccctgg cgcgtggggc ccgatgtccc ctaaccggat cgctccgcgt
    11761 cgtgaggtgc tctacaccga cggagggacc cgtgggtaga tcgcgcggca aatcgaggac
    11821 ggaaccgttc acggccatac cgcgcgacgt gctgaactcc cacgccttca aatcgctccc
    11881 cgcgatttat cgagctctct atctcgacct gcgtcgccaa gcgacaccgt ggcagaacgg
    11941 gaagattgcg gcctcagatt acacgcttgg cgtcttcggt tggtcgcatt cgacgatcca
    12001 taagggcctc gccctgctga tcgaacatgg tctcttggaa cgcacacgcc gcggtgggct
    12061 cggtgatcgg ggaaagaaac gctcgctcta cggattcacg gacatggcga ttgaagccga
    12121 cgagggtcga ggcatccgcg gaaggctgcc gacctacgca tatcggaact acaaacctgc
    12181 caaggaagtg agtgaggacc gacctcagga aataaagcaa cgtccacgca gtgaaccgtt
    12241 ccggtccata cggtgtacgt cgaaagcgga tacggtacac gcggtggact cgaaagaaac
    12301 tcaaggtcca ccaaatggac cgttgtacga gccggtcaca attcctgttt tccgagagca
    12361 aatcgccgcg taacgatgcc ggtccccagt acgttcgtca aagtcatacg gtggacacaa
    12421 ctactagagg tagccatgca gggcggcgtc ggtggcggcg gggaaccgcg atcgttagcg
    12481 aagcgagcgg atgggtcgag ccgaacttgg caacggcctc cgactgttgg tcgcaagcgc
    12541 aacggcgcga tcggacgctg tcctacggtc cgatcgggag aacgtccgct ctgcaacgct
    12601 gccccgctct cggaggtgga cgcctttcgc gttatgcagt ttcccgcatg ggacatagaa
    12661 ttggtggcgc agacagagct gcctccgctc tgagtagggc gactcgcaat catcctaggg
    12721 acgaggcatg ggaatcgtga ctagagttcc atcctgtccc attttgcgga aggggtgaca
    12781 gcggtgcgtc gacggactcg cccgcggaat cgcggactgg caccgatccg gcgggaaacc
    12841 gaccgggcga ttctcacacg cagggagcgg gagcgaaccg gaccgatgac gacgcatcac
    12901 ccgacggggg cgcgcgatga ctgaaccggc agatacgaca ccaagcggcg cgtccccgtc
    12961 gccacagacg ggcgaggcgg atgcagacgg ctcgaacccg gccggcgacg cagtggaatc
    13021 cggagacaaa tgggacgcgc tgttgaagcg catcgccgag cgtgagaaac gctacacgcc
    13081 agcgaagcgg gagatggcaa gggcgttcgg ctttctgtag cgaggcaatc aattcaaccc
    13141 gaggcaaacg cacatgcacc caatcaaagt gacaagacgg gcggcgcgtc cgggcgagag
    13201 actgttcggc gggaagggag ggctgatccc caacaggaag ccgcttgcga actcctcgac
    13261 cgagccttcg acgaagccgg ccgcgcagag ccagacgaca ccgaagtcgg atggggaatc
    13321 cccgaatgat ccgagttgaa ctcatccacg gcccccggaa ggccgtcatc gccgcgatgg
    13381 cgtggctaga tgccgtcctc ccaccgggcg cggaagcgcg cgcggcaacc ctcctgcgac
    13441 tcgcacagga cgcgggaatc gccgctacga cgctgcgtcg ggcgaagacc cgtcaacggg
    13501 tcgaatcgtg gcgggcgagt gtgcgcgtgg ggggattctg gctctggcgg cgacctagaa
    13561 gcctcggagc atccgagaga taggtagacc accctccgag cctcccgcgt cgcgctgcgg
    13621 ggctgtattg cagcgatagg gccatttgcg cccgggcgcc cgggccgccg agcgattgtc
    13681 gaaggtccgg ctcgggtatc ctttctcgcg atcgaggata acgggaggag tactggtgca
    13741 aaacgcgctc tacttcgggg acaacctcga tgttctgcgg gagtccatca aagacgagtc
    13801 gatcgatctc atctacctcg acccgccctt caacagtaac gccacgtaca acgtcctatt
    13861 caaaacgccg aagggacatc aatcagacgc gcagatcgag gcgtttgatg atacgtggca
    13921 ttgggggcca cacgccgagg cggcattcgc ggagttgatg cagcaatcga acacggacgt
    13981 tgccgaggtg acgcatgcgc tccgctccgt tctcggcgag aacgacatga tggcgtacct
    14041 cgtgatgatg gggattcggt tgcttgaatt gcaccgcgtc ttgaagccga ccgggagcct
    14101 atacctgcat tgcgacccga ccgcaagcca ctacctgaaa atcgtactcg accaagtgtt
    14161 ctcgccagag ttcttccgca atgaaatcgt ctggcagcgg acaaacgtgc atagcgacag
    14221 caagacgtgg agccgcgtga gcgattcaat cctgttctat acgcgcagca acgccttcac
    14281 ttggaaccca atctacctac cgcatagcga cgaacacatc gaggcgaagt accgcagcgc
    14341 cgatccagat gggcggcgct acacccttag cgacatgacg agtcccgcgc cacgaccgaa
    14401 catgatgtac gtgtggcggg gttttccgac cccgaatttc ggatggcggt attctaagga
    14461 gacagtggag aagttggatg cagaaggacg catctggtat cccaccttca agaacggcca
    14521 gcgcgacacc tccaagcggc cacggctcaa acgctacttg gacgagatgc cgggaacgct
    14581 cctgacgaac gtctggacgg acatctatcc gatcaactcg caggcccagg agcgccttgg
    14641 ctacccgacg caaaagccgc tcgccctgct tgagcggatt atcgtcgcgt cgtccaacga
    14701 agatgatctt gtcctcgacc cgttctgcgg gtgcggcacg gcggtcgatg ctgccgagaa
    14761 actgaaacgg cgctgggtcg gaatcgacgt tactcacctc gcggtttcgc tgatcgaaaa
    14821 gcggatgcgc gaccggtacc cgaaaattga gttcgtcgtg cacggtacgc ccaaagacct
    14881 cgatggcgcc cgcgacctag caacgcgcga caagtatcaa ttccaatggt gggcttgctc
    14941 actagtaaat gcgcaaccgt ttcaaggcag gaagaagggc gccgattccg gcatcgatgg
    15001 catcatctac ttccaagacg acgacaagtt gccgaagaag atagttgtca gcgtaaaggg
    15061 cggcgataac gtcaacgtag cgatggtccg cgacttcgcc catgtgatcg agcgtgagaa
    15121 ggcggctatg ggcttcttcg tgacgctgac agacccgaca aagaacatgg tgaccgagag
    15181 cgtcggcacc ggctactaca cctcgccgct gactggggcg aaatatccga agttgcaggt
    15241 tctcaccatt gaaggtctac tttccggcca agaacgcccc gcctatcccc gcatggatgc
    15301 aggcgggctg acgttcaaga aggcgaagga agccgcgcca gagcagaagc aactgctttg
    15361 aggtacgcat gtttgtaacg ggacccgcga gttacaccag tgacgaactc gctgcgttga
    15421 atctggacgc ggcgatcgaa atgtatcttg atggtaggtt cattccggcg atcgtactgg
    15481 ctaccgcggc agaaatgcag ttcggcggtc cgcatgcgcc gagaacacgg gacgatcgga
    15541 cgatcgatcc gagagcacac cagcgagacg ctcgggaact cgctgaa
//
LOCUS       DASUCJ010000123         5926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_811206, whole genome shotgun sequence.
ACCESSION   DASUCJ010000123 DASUCJ010000000
VERSION     DASUCJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811206"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..1681)
                     /locus_tag="VFU90_01545"
     CDS             complement(389..1681)
                     /locus_tag="VFU90_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5478479.1"
                     /translation="MKRLSTGVLAVLMAAGLGLNAVAAQSHASHGNIGTNAVAQESSP
                     APMAAPPANFGSPPSGQIPILYNDHHVYTKPDVLKQSRVLAALVRGGTILIPLRSMFE
                     QMGATVSYDAGSKTVTVSKAGAEVKVTVGKPEVIINGESRPLDVPPIMYQGNVLVPVR
                     VISEGMGAYVLWVGNKQRVVVVRYIVPTPPPPPATAPPPPPTPTPTPMATPYHDLYVA
                     GDYLISPKVYNEFSPGTTACNSCNGFSWRLHGAVEFDLLNLPWMVEGDYRQYNYQHPT
                     GPVTAIGGQFQTFVPAFNVRDYDLDARFGLRVLQPRIYIGVGYIWRSNNAGYPGVNGV
                     GFGLEKLPDLDRPFSFYGSAYYFPSVKGTCGTGECPTGPFTLGYNVLKYDLGVDYSFQ
                     GIPLFLEGGYLGEHGWATDSSPSPYAESGPYVGIGVKF"
     gene            complement(1858..2253)
                     /locus_tag="VFU90_01550"
     CDS             complement(1858..2253)
                     /locus_tag="VFU90_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEU5478480.1"
                     /translation="MTAKLDIGRSYSLQSRVEEWMTAEKAGNKGADVLSTSVLVQLVE
                     SAAVHCIDPVLRSDQISLGTHIDLEHHKPVPVGFIVRTEVEVVMIDGPRVSFAVQVFD
                     EREAVAEGAHERYIIDRAKFLAKLEEKLT"
     gene            2339..3667
                     /locus_tag="VFU90_01555"
     CDS             2339..3667
                     /locus_tag="VFU90_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5478481.1"
                     /translation="MNQKRAGSVYDSVTGIHQSRRNMQYRRLAMTALAAALALDACGG
                     NGAPKGPPPLSVDVAAATRQNIATYVTLDGQIAPKEQSTLAFQQSGTIVKVLVNIGDL
                     VRKGTLLAQIDPSTLSAQLSQAQAQAAQAGASAQGAVVGLPVQYQSNNAAVATAKANL
                     ENAKLVYEQNKQLFKQGYVSQTALQQSQASYVQAQSTYNNAVVALRNNVVSAENVKAQ
                     LAQAQAARAQANVLSTQLSQTSLYAPFDGVISNRLLDPGAYASPQQPVLTISRVDIVW
                     ININVPDEDLAYVRPGTMVRFKTSSLGNREFDGRIQTVNAVPTAGTLSYLARLQMSNP
                     GDVLRGGMLISATLPKQSQSDAIVVPRSAVAQTENGSVVYVVNGDKAEGVPVKVGVET
                     DTLAQVISPKVQPGTKVITTRPDALKDGSVVAVNGPPGASPSPSAGGTHQ"
     gene            3664..5154
                     /locus_tag="VFU90_01560"
     CDS             3664..5154
                     /locus_tag="VFU90_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5478482.1"
                     /translation="MTVRSIFARALIGALALGAALPNAGLAQPVPSGQQIIMPTPAPM
                     PTMGGTPLPYPAYGTPAPDVAHTTAKPGVPTSVTLSQAVKIAVALSPVFAGQNAQWAA
                     IHAKYTSEKQALFPAVSGNGTIGNQYENQTAVRNGSVFPTASPGPATLGANGTTASES
                     LSITVQQLIYDGGRTIAAIRSAKESDYAGRATLFRDLETLAFNVATAYYGVLQANATV
                     TADAQLVKEFEVSQAAVAAQIRNGAAARSDIASAEFQTATARGNLVTAQGQAIGAQAT
                     FATVLGLDADADVVPHSLGKPNTFANPAYQASLKRAFVLRPDFLAAQYTVEADKENLR
                     YAKLARFPELTAGASDQVGKTFGPFSSGYTNTKTLGLNLTIPIYDQGLTNYNVAVAAA
                     TLDGANSSLQQTRLQVESDVRSALANLISARALLVQTQAELNAAKVSLDATQAQYRVG
                     ATTIINVTTAEANLATAQSDQIKAFYGEQTAEQNYLYATGTTDLEI"
     gene            5200..>5926
                     /locus_tag="VFU90_01565"
     CDS             5200..>5926
                     /locus_tag="VFU90_01565"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478483.1"
                     /translation="MVSNASAVPAAVSDPVNAAILAISEDRLAGFQTDPFGEIARRSG
                     IGADVVRERIVAMLRAGTIRRVRQTVLSTSLARGALCAWQISEERLDAAFDYMFRNDP
                     FSGHVVIRSTDAASPGSTYRLWTTLKVPQGFSLRKHAELLRVQTGAERVRLMPAKMLF
                     ALGVGHVRRRGLEPGARAPEPAQPQDTAFVALSDLEWRVLTALKREFAPDEIVADLWR
                     ARAAEAGVDLETFFGVAAALNERR"
BASE COUNT         1106 a         1950 c         1898 g          972 t
ORIGIN      
        1 cgacgcacac cctgcgcacc gatctcggcc ccttccacga gtaaagccgg tcgcggctcg
       61 tcccaccctg tcatcctgag cggagcgccg caggcgcgca gtcgagggac gatcagcctt
      121 aaacgcgcac gacggtgggg gtgccgtgag cgaggcgtgc gactttttcg caaaagccag
      181 gacggcgtat tcctttggtg ttgcgaaaaa gcgcacgagt aggagcgagt catggacgcg
      241 agcgacgaac gtccgagcga tggcacccca ccgccgcgct ttggcgccct gaccgcggtg
      301 ccgcgcgagc tgcgcgcggg atgacgagta aaaagaagaa gcccccggcg gtgccgaggg
      361 cttcgttcat ctcgggctcg tcgcgaagtt agaacttcac gccgataccg acgtacggtc
      421 cgctctccgc gtacggagac ggcgacgagt cggtagccca gccgtgctcg cccaggtagc
      481 cgccttcgag gaagagcggg atgccttgga acgagtagtc aacgcctaag tcgtacttca
      541 ggacgttgta tccgagcgtg aaaggtccgg tcggacattc gccggtgccg cacgttccct
      601 taaccgatgg gaagtagtac gcgctcccgt agaacgagaa cggacgatcc agatccggca
      661 gtttctcgag tccgaaaccg acgccattga cgcccgggta tccggcgttg ttggaacgcc
      721 agatgtagcc gacgccgatg tagatgcgcg gttgcagcac gcgaaggccg aagcgcgcgt
      781 cgagatcgta gtcgcgaacg ttgaacgccg gaacgaacgt ctggaactga ccgccgatcg
      841 cggtgacggg tccggtcgga tgctggtagt tgtactgacg atagtcgcct tcgaccatcc
      901 acggcaggtt caggagatcg aactccacgg cgccgtgtag acgccacgag aatccgttgc
      961 acgaattgca tgccgtggtg ccggggctga actcgttgta gaccttcggc gagatgaggt
     1021 aatcaccagc gacgtaaagg tcgtgatacg gggtagccat cggcgtcggg gtcggcgtcg
     1081 gcggcggcgg cggggccgtt gccggcggcg gcggcggagt cggcacgatg taccgcacca
     1141 cgacgacgcg ctgtttgttg cctacccaca gaacgtacgc tcccatgcct tccgaaatga
     1201 cgcggaccgg cacgagcacg ttgccctggt acatgatcgg cggaacgtcc agcggacgcg
     1261 actcgccgtt gatgatgact tccggcttac cgacggtcac tttgacctcg gctccggctt
     1321 tgctgaccgt cacggtcttg ctgcccgcat cgtacgacac ggtagcaccc atttgctcga
     1381 acatcgagcg aagcggaatc aggatcgtgc cgccgcgcac gagcgcagcg agcacgcggc
     1441 tctgcttcag gacatcgggc ttggtataga cgtggtggtc attgtagaga atcgggatct
     1501 gacccgacgg cggggatcca aaattcgccg gcggagcagc catgggggcc gggcttgact
     1561 cctgcgcgac cgcgttggtt ccaatgttgc catgcgacgc gtgggattgg gccgcgaccg
     1621 cgttgagccc tagaccggct gccatcagca cggccagaac tccggtgctc agtcgcttca
     1681 atgtgtcctc ctagctagct agttattgag acctcagctt gcgcctggcc ccaagccccc
     1741 tcgggcgccg gggggtccgg gcgcagtcct gctgtgtctt tttcgggaac aaccgattgc
     1801 cccccctgca gcgctagccg ggggaggccg gggacggggt ccatccttac cctaccgtta
     1861 tgtaagcttt tcttcaagct tggcgaggaa cttggcccgg tcgatgatat agcgctcgtg
     1921 agcgccctcc gcgacggcct cgcgctcgtc gaaaacctgg accgcgaagc tcactcgcgg
     1981 gccgtcgatc atcacgacct ctacttccgt ccgcacgata aagccgacgg gcaccggctt
     2041 gtggtgttcg aggtcgatat gcgtgccgag gctgatttgg tcggaccgca gcaccggatc
     2101 gatacaatgg acggccgcac tttccactaa ttggaccagc accgacgtgg agaggacgtc
     2161 ggcacccttg ttgcccgcct tctcggcggt catccactct tcgacgcggc tctgcaggct
     2221 gtacgaacgg ccgatgtcga gttttgcggt catcccgggg gaaagtacgc cctgtagtcg
     2281 gccacctcat tcccaagatg tgtgttaggg gcaatgccaa gggttcgagg gttagggtat
     2341 gaatcaaaag cgggccggca gcgtatatga cagcgtaacc ggcattcacc aaagtaggag
     2401 aaacatgcag tatcgacggc tggcgatgac cgccctcgcg gcggcgctgg cactggacgc
     2461 gtgcgggggg aacggagcgc cgaagggtcc gcctccgctc agcgtggacg tggccgcggc
     2521 cacgcgccaa aacatcgcga cgtacgtgac cctcgacggc cagatcgcgc ccaaggaaca
     2581 atcgacgctc gcattccagc agagcggcac gatcgttaag gtcctggtga acatcggcga
     2641 tctcgtccgg aaggggacgc tgctcgcgca gatcgatccg tcgacgcttt cggctcagct
     2701 ctcccaggcg caggcgcaag ccgctcaggc cggggcctcc gcgcagggcg cggtcgtcgg
     2761 tctgccggtg cagtatcaat cgaataacgc cgccgtcgcg accgccaagg ccaacctcga
     2821 aaacgccaag ctcgtctacg agcaaaacaa acagctgttc aaacagggat acgtttcgca
     2881 gacggcgctg caacagtcgc aggcgtcgta cgttcaggcg cagtcgacct acaacaacgc
     2941 cgtcgtcgcg ctgcgcaaca acgtcgtgag cgccgaaaac gtcaaggcgc aactcgcgca
     3001 agcacaagcc gcgcgcgcgc aagccaacgt cctaagcacg cagctttcgc agacgtcgct
     3061 ctacgcgccg ttcgacggcg tgatctcgaa ccgtttgctc gatccgggcg cctacgccag
     3121 cccgcagcag ccggtcttga cgatttcgcg ggtcgacatc gtgtggatca acatcaacgt
     3181 cccggacgaa gatctagcgt acgtacgccc cggtacgatg gtgcgcttca agacgtcctc
     3241 gctgggcaac cgcgagttcg acggccgaat ccagacggtc aacgccgtgc cgaccgcggg
     3301 aacgctgtcg tacctggcgc ggctgcaaat gtccaatccc ggcgacgtgc tgcgcggcgg
     3361 catgctgatc agcgcgacgc tgccgaagca gtcgcaaagc gacgcgatcg tcgtcccgcg
     3421 cagcgccgtg gcgcaaaccg agaacggcag cgtggtctac gtcgtcaacg gcgacaaggc
     3481 cgagggcgtg ccggtgaagg tcggcgtgga aaccgacacg ctcgcgcaag tcattagtcc
     3541 gaaggtgcag cccggcacga aggtcatcac gacccgtccg gatgcgctca aagacggcag
     3601 cgtggtggcg gtcaacgggc cgcccggggc cagtccgagc ccgagcgccg gcggaacgca
     3661 ccaatgacgg tgcgttccat ctttgcgcgc gcgctgatcg gagcgctcgc gttgggcgcc
     3721 gcactgccca acgccggcct cgcgcaaccg gtgccctccg gacagcagat catcatgccg
     3781 acgccggccc cgatgccgac gatgggcggc accccgctgc cgtatccggc ctacgggacg
     3841 cccgctcccg acgtcgcgca tacgaccgcc aaaccgggcg ttccgacttc ggtcacgctc
     3901 agccaagcgg tgaagatcgc ggtggcgctc tcgccggtgt tcgccgggca gaacgcgcag
     3961 tgggccgcga ttcacgccaa gtacacctcg gagaaacaag cgctgttccc ggccgtgagc
     4021 ggtaacggca cgatcggcaa tcaatacgaa aaccaaacgg ccgtgcgcaa cggctcggtc
     4081 tttccgaccg ccagtcccgg gccggcgacg ctgggtgcca acgggacgac ggcgtcggaa
     4141 tcgctctcga tcacggtcca gcagctgatc tacgacggcg ggcgtacgat cgcggccatt
     4201 cgctcggcca aagaaagcga ctacgccgga cgggcaacgc ttttccggga tctcgaaacg
     4261 ctcgcgttca acgtcgccac cgcatattac ggcgtgctgc aggccaacgc gacggtgacc
     4321 gccgatgcgc agctggtcaa agagttcgaa gtcagccaag ccgcggtcgc cgcgcaaatt
     4381 cgtaacggtg cggcggcacg gtccgacatc gcgtcggccg aattccagac cgctaccgcg
     4441 cgcggcaacc tcgtgaccgc gcagggtcag gcgatcggcg cgcaggcgac gttcgcgacg
     4501 gtgctcggcc tggacgccga cgccgacgtc gttccgcaca gcctcgggaa acccaatacc
     4561 ttcgcgaatc cggcctatca agcgtcgctc aagcgcgcct tcgtgctgcg cccagacttt
     4621 ctagccgcgc agtatacggt cgaggccgac aaggagaatc tccgctacgc caagctggcg
     4681 cgctttccgg agctcaccgc gggcgcgagc gaccaggtcg gcaagacgtt cggccccttc
     4741 tcttccggtt ataccaatac gaagacgctc ggcttgaacc tcaccattcc gatctacgat
     4801 caggggctca cgaactataa cgtcgcggtg gcggcggcta ccctcgacgg cgccaacagc
     4861 tcgctgcagc aaacccggct gcaggtggaa tcggacgtgc ggtcggcgct ggccaatctg
     4921 atttcggccc gggcgctgct cgtgcagacg caagccgaac tcaacgccgc caaagtttcg
     4981 ctggacgcga cccaggcgca gtaccgcgtc ggcgccacca cgatcatcaa cgtcaccacc
     5041 gcggaagcga acctagcgac cgcgcaatca gatcaaatca aagccttcta cggcgagcaa
     5101 accgccgaac aaaactacct gtacgctacc ggcacgaccg acctggagat ctagccctcc
     5161 gcacagggca cgcagcggac gaacggaaac tacttcgccg tggtttccaa tgcttcggcg
     5221 gtcccggccg cggtctcgga cccggtcaac gccgcgatct tggcgatttc cgaagaccgg
     5281 ctcgccggtt ttcaaaccga tcccttcggc gaaatcgcgc ggcgttcggg gatcggcgcc
     5341 gacgtcgttc gcgagcggat cgtcgccatg ctgcgcgccg ggacgatccg gcgcgttcgt
     5401 cagacggtgt tgtctacgag tttggcgcgc ggcgcgctgt gcgcgtggca aattagcgaa
     5461 gagcggctcg atgcggcctt cgactacatg tttcgcaacg accctttctc gggtcacgtc
     5521 gtcatccgct cgaccgacgc cgcttcgccg gggagcacct accgtctctg gacgacgctg
     5581 aaagttccgc agggcttctc gctgcgcaaa cacgccgagc tgctgcgcgt gcaaaccggc
     5641 gccgagcgcg ttcgcctgat gcccgcaaag atgctgttcg ctctcggcgt cgggcacgtg
     5701 cgccggcggg gcctcgagcc gggcgcccgc gcccccgaac ccgcgcagcc gcaggatacc
     5761 gcgttcgtcg ccctttccga tctcgaatgg cgggtattga ccgcgctgaa acgcgagttc
     5821 gctccggacg agatcgttgc cgacctttgg cgcgcgcgcg ccgccgaagc cggcgtcgat
     5881 ctcgagacgt tcttcggcgt cgctgcggcg ctgaacgagc ggcgcg
//
LOCUS       DASUCJ010000124         1947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_658384, whole genome shotgun sequence.
ACCESSION   DASUCJ010000124 DASUCJ010000000
VERSION     DASUCJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658384"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFU90_01570"
     CDS             complement(<1..1576)
                     /locus_tag="VFU90_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5478484.1"
                     /translation="MAKSSPRPPSSASAAPLVAVLLLTLGVAGAITWVAWLSTSSHRA
                     AAQDAMRDYAEFAAANFTDAAGKALYGGSMAILSSVGAASATMEADHVYPLTVLEHAA
                     GKMRDWGWMYVPTPRYVFSYDLSTHKVQVGGENTPGQVEQARLLDSLSANFDTVSTVT
                     SGADPRARSLYVMIDSTTHDTGIYLMVFMRGVHGAPNMVYGFSTLFRNYAQTVLPQLA
                     KATPLVPRSLAHGLPNDSLLTISVRDAGGRTVYESSTAGLTAFGATHQLWSFAEGPTV
                     QIAVRKHAADRLLRGGIPRNRVPLLVALLLCTGALVVVAFHLARRAEELAHLRADFTS
                     SMSHELRTPLAQIVLFAESLSFGRVTTERGRSDALRVILREARRLGHLVDNVLLFSRT
                     ERRGTHVSAQARPLAPLVREIVESFEPLAKVRQSSLRLSLDENVVAPVDEGALRQILL
                     NLLDNAVKYGPDGQTVAVGLSLERGRAVLCVDDEGDGVPESESKRIWDPFVRLHSGEY
                     SVSTGSGIGLSVVRQLV"
     gene            complement(1762..>1947)
                     /locus_tag="VFU90_01575"
     CDS             complement(1762..>1947)
                     /locus_tag="VFU90_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478485.1"
                     /translation="GQGKIGIEPFRWLLADRRARGVPLILETPHREGSDAEDNLAPDP
                     NDVEMLQLLRGMMLSNA"
BASE COUNT          358 a          664 c          617 g          308 t
ORIGIN      
        1 tgactaactg tcgcaccact gagagcccga tgccgctccc ggtggacacg gaatactcgc
       61 cactgtgaag ccgtacgaac ggatcccaga ttcgtttact ctcggactcc ggcaccccgt
      121 cgccttcgtc atccacacac agcaccgccc gaccgcgctc caggctgaga cccacggcaa
      181 ccgtctggcc gtccggaccg tacttcacgg cgttgtccaa cagattgagc agaatctggc
      241 gcagtgcgcc ctcgtccacg ggcgccacca cgttctcgtc taacgacaat cgcagcgacg
      301 actgacgcac tttcgccaac ggctcgaacg actcgacgat ctcgcgcacc aacggcgcca
      361 gcggcctcgc ctgcgccgac acgtgcgtcc cgcggcgttc cgtccgcgag aagagcagca
      421 cgttgtcgac gagatgaccc agtcgccgcg cctcgcggag gatcacccgc agcgcatcgc
      481 tgcgaccgcg ttccgtcgtc acccggccga acgacagcga ttcggcgaac aacacgatct
      541 gggcgagagg cgtccgcagc tcgtgcgaca tgctcgaggt gaaatccgca cggagatgcg
      601 cgagttcctc ggcccgccgg gcgaggtgga acgcgacgac caccagggcg cccgtgcaca
      661 ggagcagcgc caccagcagc ggcacgcgat tccgtgggat gccgccgcgc aacaacctgt
      721 cggcggcgtg cttccgaacg gcgatctgga cggtcggccc ttcggcaaac gaccacaact
      781 ggtgcgtcgc gccgaacgcc gtcagaccgg ccgtcgacga ttcgtagacc gtgcgaccgc
      841 cggcatcgcg cacgctgatc gtcagcagtg aatcgttagg cagaccgtgc gccagcgacc
      901 gcggcacgag cggcgtggcc ttggcgagct ggggcagaac ggtttgcgcg tagttgcgga
      961 acagcgtcga gaacccgtag accatgttcg gcgcgccgtg cacgccgcgc atgaacacca
     1021 tcaggtagat gccggtgtcg tgcgtcgtgc tgtcgatcat cacgtacagc gagcgtgcgc
     1081 gagggtcggc gccgctcgtc acggtgctga cggtgtcgaa gttggccgac agcgaatcga
     1141 gcagccgcgc ctgctcgact tgtcccggcg tgttctcgcc gcccacttgc accttgtgcg
     1201 tggacagatc gtagctgaac acatagcgcg gcgtcgggac gtacatccaa ccccaatcac
     1261 gcattttgcc cgcagcgtgc tcgagcaccg tgagcggata gacgtggtcc gcttccatcg
     1321 tcgccgaggc ggcgccgacg gacgacagga tggccatgga gccgccgtag agcgccttgc
     1381 ccgccgcgtc ggtgaagttc gcagcggcaa attcagcgta atcgcgcatc gcgtcttggg
     1441 cggccgcgcg atgcgagctg gtcgagagcc aggcaaccca cgtgatcgcg ccggcgacgc
     1501 caagcgtcag cagcaggacg gcaaccagcg gagccgcaga tgcactggac ggcgggcgcg
     1561 gcgaactctt ggccatgcta tccctcgtgt tacgaaacac tcgcgctgtc tcgaagctgc
     1621 ggaacttcga ctatgtacag cgcaacgcac ggccgggttc agcctgacga agaccggacg
     1681 tggtccggac ttcatttgga cgccggcgcg ctccacttgg tttacatacg acgagcgata
     1741 gaacgcagtt tcgcgctcgc gtcaggcatt ggacagcatc atgccgcgca agagctgcaa
     1801 catttcgacg tcgttcggat ctggtgcgag attgtcctcg gcgtcggagc cctcccggtg
     1861 cggcgtctcg aggatgagcg ggacgcccct cgcacgccga tcggccagca gccatcggaa
     1921 cggctcgatt ccaatcttgc cctgacc
//
LOCUS       DASUCJ010000125         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_200004, whole genome shotgun sequence.
ACCESSION   DASUCJ010000125 DASUCJ010000000
VERSION     DASUCJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200004"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /gene="hmgA"
                     /locus_tag="VFU90_01580"
     CDS             <1..1170
                     /gene="hmgA"
                     /locus_tag="VFU90_01580"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU5478486.1"
                     /translation="GSAFTAPRGANRRSWLYRIRPAAVHLPFQRVDNGRYDGDFAAAA
                     TPPNQLRWDPLPVPTAATDFIAGIVTMAGNGDPYTQTGCAIHVYAANQSMTDRYFYSA
                     DGEMLIVPHEGRQRFVTELGVIEAEPQEIVVIPRGVRFRVELVDAIARGYICENFGAP
                     FRLPEMGPIGSNGLAHPRDFLIPVAWYEDRDAKSELVARFMGNLWSAEMDHSPLDVVA
                     WHGNDAPYKYDLRRFNAIGSVSYDHPDPSIFLVLQSQSDTPGVDTIDFAIFPPRILAM
                     EHTFRPPWFHRNVASEFMGLVRGVYDAKAEGFVPGGASLHNCMTAHGPDAQTFEKATT
                     ADVSRPDYIRDTMAIMFETRCVIRPTRFALETPQLQAGYYRCWQDLRRHFDPQRR"
BASE COUNT          213 a          440 c          392 g          228 t
ORIGIN      
        1 ggaagcgcgt tcactgcgcc gcgcggcgcc aaccggcgct cgtggctgta ccggatccgc
       61 ccggcagcgg ttcaccttcc gttccaacgc gtcgacaacg gccgctacga cggcgatttc
      121 gccgccgccg cgacgccgcc caatcagttg cgctgggatc cgctgcccgt cccgactgcg
      181 gccaccgatt tcattgccgg catcgtgacg atggccggca acggcgatcc gtacacgcag
      241 accggttgtg cgattcacgt ctatgccgcg aaccagtcga tgaccgatcg ctatttctac
      301 agcgccgacg gcgagatgct gatcgttccc cacgaagggc ggcagcgctt cgtcaccgaa
      361 ctcggcgtca tcgaagccga gccgcaggag atcgtcgtca ttccgcgcgg cgtgcgcttt
      421 cgcgtcgagc tcgtcgacgc aatcgcgcgc ggctacatct gcgagaactt cggcgcgccg
      481 tttcgtcttc ccgagatggg gccgatcggc tccaacggtc tcgcccatcc gcgcgatttc
      541 ctgatcccgg ttgcatggta cgaggaccgc gatgcgaagt ccgagctcgt cgcgcgcttc
      601 atgggcaatc tctggtcggc cgagatggat cactcgccgc tcgacgtggt cgcgtggcac
      661 ggcaacgacg caccgtacaa gtacgacctg cgtcgcttca acgcgatcgg ctcggtgagc
      721 tacgatcatc ctgatccgtc gatcttcctc gtcctgcagt cgcagtcgga tacgccgggc
      781 gtcgatacga tcgacttcgc gatcttccca ccgcgaatcc tcgcgatgga gcacacgttc
      841 cgcccgccgt ggttccatcg caacgtcgcg agcgaattca tgggtctcgt gcgcggcgtc
      901 tacgacgcga aagccgaagg gttcgtgccg ggtggcgcga gcctgcacaa ctgcatgacc
      961 gcgcacggcc ccgacgcgca gacgttcgaa aaggcgacca ccgccgacgt gagccgtccc
     1021 gactacatcc gcgacacgat ggcgatcatg ttcgagacgc gctgcgtgat ccggccgacg
     1081 cgctttgcgc tcgaaacgcc gcagttgcag gccgggtact atcgctgctg gcaggacttg
     1141 cgcaggcact tcgatccgca gcgacgatga cgacgttgaa cgaaacgcac gaccccgcgc
     1201 tcgagtgctg ggtcgaatcc gcgcggggtc ccgacggtga tttcccgctg cagaacctcc
     1261 cgttcgcgat att
//
LOCUS       DASUCJ010000126         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_169458, whole genome shotgun sequence.
ACCESSION   DASUCJ010000126 DASUCJ010000000
VERSION     DASUCJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169458"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFU90_01585"
     CDS             complement(<1..655)
                     /locus_tag="VFU90_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5478487.1"
                     /translation="MTAPNNRERIVSRLIDEEIKESFINYSMSVIVSRALPDVRDGLK
                     PVHRRVLYAMNELGLVPGRPYKKSATVVGDVLGKYHPHGDISVYDALVRMVQDFSLRY
                     PLVDGQGNFGSVDGDNAAAYRYTEARLMALATELLADIDKNTVDFSPNFDDRLQEPKV
                     LPAGFPNLLVNGSSGIAVGMATNIPPHNLREIVGAIVHLVDNPEATPADLRKYIKGPD
                     "
     gene            complement(803..>1073)
                     /locus_tag="VFU90_01590"
     CDS             complement(803..>1073)
                     /locus_tag="VFU90_01590"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5478488.1"
                     /translation="SSVLASPFVGYKSPLLFERRYDPQFSTRLMLKDLDLVLASAAEH
                     GLALPATAIIREQFARAVAAGLGDDDAAAVREVVDGDGKAPRARR"
BASE COUNT          194 a          353 c          346 g          180 t
ORIGIN      
        1 agtcgggacc cttgatgtac ttccgaagat cggccggcgt cgcctcgggg ttgtcgacga
       61 gatggacgat cgcgccgacg atctctcgca ggttgtgcgg cggaatgttc gtcgccatgc
      121 ccaccgcgat gcccgacgaa ccgttcacca gcaggttcgg gaatccggcg gggaggacct
      181 tgggctcctg cagccggtcg tcgaagttag gcgagaagtc gaccgtgttc ttgtcgatgt
      241 cggccaggag ctcggtcgcc agcgccatga gccgcgcttc ggtataccgg tacgcggccg
      301 cgttgtcgcc atccaccgat ccaaaattgc cctgcccgtc caccaggggg taccgcagcg
      361 aaaaatcctg caccatgcgc acgagcgcgt cgtacacgct gatgtcgccg tgcgggtggt
      421 acttgcctaa cacatcgccg acgaccgtcg ccgacttctt gtaaggccgg cccggcacga
      481 ggccgagctc gttcatcgcg tacagcacgc ggcgatgcac gggcttgagt ccatcgcgca
      541 catccggcaa cgcgcgcgac acgatcacgc tcatcgaata gttgatgaac gactctttga
      601 tctcttcgtc gatgagccgg gaaacgatcc gttcgcggtt gtttggggcg gtcatgcggg
      661 tacggcggag gagaaagaac ggggggaggt catagcacag aaatctaatc cgtgcggagc
      721 ccctggacaa ccgcggcggg acaatctaag ccgttgtagg cgcgccggtt acgaagctga
      781 ctcagccgcg gctggcgccc cctcagcgac gcgcgcgcgg agccttccca tcgccgtcca
      841 ccacctcacg gacggcggcc gcgtcgtcgt cgcctaaccc cgccgccacc gcgcgcgcaa
      901 actgttcccg aatgatcgcc gtcgccggca gcgcgagccc atgctcggcc gcgctcgcga
      961 gcacgagatc cagatccttg agcatgagcc gcgtgctgaa ctgcgggtcg tatcgtcgct
     1021 cgaagagcag cggcgacttg tagcccacga acggagacgc gagcacggag ctc
//
LOCUS       DASUCJ010000127         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1422707, whole genome shotgun sequence.
ACCESSION   DASUCJ010000127 DASUCJ010000000
VERSION     DASUCJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422707"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFU90_01595"
     CDS             complement(<1..477)
                     /locus_tag="VFU90_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5478489.1"
                     /translation="MIASAMLHATLAGTLVAIVIAVLVCAILYWMLHAPPSGLEKLAA
                     KTTGQLKQMVGSIIVVFSQEINSGHMMALASKLARGERSELLAVYVIEVPRTLPPDAE
                     MPDEERAALDALGAAETIANKDNVTLRTQTIKTRSTREAVLDIAKREKADLIILGSY"
     gene            complement(474..662)
                     /locus_tag="VFU90_01600"
     CDS             complement(474..662)
                     /locus_tag="VFU90_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478490.1"
                     /translation="MLAVRAVLAAALIVCGTVILVRVASLGLRFEGLPGYVLGAAMIA
                     LGVHRLSLIVRAAGGAAR"
     gene            complement(664..2280)
                     /locus_tag="VFU90_01605"
     CDS             complement(664..2280)
                     /locus_tag="VFU90_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5478491.1"
                     /translation="MPSTKPELRRTVTPWGSFSWGYSDVGADIFVGLGLVLAAAAGAS
                     NVAFLFAGIVYVCIGLAYTELAATYPVAGGGQYFVLRGLGDIFGFIAGWAVLLDFTID
                     ITLFAWTCVDYLSQLAPVLTGSIHPWVHFLVTLGFIVALATLNVVGVRESTAFNGFVS
                     ALDVISETCILCFGFLFAFRPELLVHNMQHAWPTPYQLMLGTSLAIISFVGLESISQA
                     AQETQRPASIIPRTSISLILTILIFALAYSNLALGMQPRHPILDAHGHLLQFWQIFPN
                     NGDNQGKAVALLAEQVPVWGALAAFYVPVLGAVLLLISSNSGVFGSSRIAYAMSKSNL
                     LPSMFQRVHSRFRTPAISIVTFGGVAVIELLFAAFPSLHPSAMTLYARFFRNESGLDF
                     LADLYAFGAATSYSFVFVALIALRLFDPLSPRKFKIPLNVPVKVRGERADFPLVAVLG
                     FLGIFSILLFTLFTHPIGRIAGPSWLVLGFIGYVIYRRHRGMPVFGSQKRDWRKEQVE
                     ILRSAGELELMDDYVAALKARDERRAGAEA"
     gene            complement(2290..>2632)
                     /locus_tag="VFU90_01610"
     CDS             complement(2290..>2632)
                     /locus_tag="VFU90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5478492.1"
                     /translation="NAASTAAKGRAGEERAVAFLESCGYRVIARNVRLPGGEIDAVCL
                     DGATLVIVEVKRRDGAAFGPALGAVGARKRATLRRLAADYAQVVAPSAAIRFDVVALD
                     GNRLRLHRNAF"
BASE COUNT          540 a          828 c          876 g          388 t
ORIGIN      
        1 ataggaacct agaattatca ggtcggcttt ctcgcgcttg gcgatgtcga gaacggcttc
       61 gcgcgtcgag cgcgtcttga tcgtttgagt gcggagcgtc acgttgtctt tgtttgcaat
      121 cgtttcggcc gcgcccaagg cgtcgagagc ggcccgttcc tcgtcgggca tttccgcatc
      181 cggcggcagc gtccgcggca cctcgataac gtaaacggcc agcaattcgg atcgctcccc
      241 gcgcgcgagc ttcgacgcga gcgccatcat atgcccggag ttgatctctt gtgaaaacac
      301 cacgatgatg ctgccgacca tctgcttgag ctgaccggtc gttttggcgg ccagcttttc
      361 cagcccggaa ggcggcgcat gaagcatcca gtagaggatc gcgcacacca gcacggcgat
      421 gacgatcgcg acgagcgtgc ccgcgagcgt cgcgtgcagc atcgccgatg cgatcatcgc
      481 gcggctccgc cggcggcgcg cacgatcagc gagaggcggt gtacgccgag cgcgatcatt
      541 gccgccccga gcacgtaacc gggaaggcct tcaaaccgca gaccgagcga ggcgacgcgt
      601 acgaggataa cggtgccgca aacgatcaac gccgcggcca gcaccgcgcg caccgccagc
      661 acgtcaggct tccgctccgg cgcgccgctc gtccctggct ttgagcgcgg ccacgtaatc
      721 gtccattagc tccaactcgc cggcgctgcg cagaatctcg acctgctcct tgcgccagtc
      781 gcgcttttgc gagccgaaca ccggcattcc gcgatgccgg cggtagatca cgtagccgat
      841 gaagccgagg acgagccagc tgggaccggc gatccggccg atcggatgcg tgaacagcgt
      901 gaataagaga atcgagaaga taccgaggaa gccgagcacc gcgaccagcg ggaagtcggc
      961 acgctcgccc cggaccttca ccggaacgtt gagcggaatt ttgaacttcc gggggctcag
     1021 cggatcgaac agccgcagcg cgatcagcgc cacgaacacg aacgagtagc tggtggcggc
     1081 gccgaacgcg tagaggtcgg cgagaaagtc gaggccgctc tcgttgcgga aaaagcgcgc
     1141 gtacagcgtc atcgcgctgg gatgcagcga gggaaaggcg gcaaacagca gctcgatcac
     1201 cgcgaccccg ccgaacgtca cgatcgaaat ggcgggcgtc cggaagcggg agtgaacgcg
     1261 ctgaaacatc gacggcagca ggttcgactt gctcatcgcg taggcgatgc gcgagcttcc
     1321 gaaaaccccc gagttcgacg aaatcaacaa caagaccgcg cccagcaccg gcacgtagaa
     1381 cgcggcgagc gcgccccaca ccggcacctg ctcggccagc agcgcgacgg ccttgccttg
     1441 attgtcgccg ttattgggga agatctgcca gaactgcagc aaatggccgt gagcgtccaa
     1501 aatcggatgg cgcggctgca tcccgagagc gaggttcgag taggcgagcg caaagatcag
     1561 gatcgtgagg atcagcgaga tcgaggttcg cggaatgatc gaagcggggc gctgcgtttc
     1621 ctgcgccgcc tgcgagatcg actcgaggcc gacgaacgag atgatcgcca gggacgtccc
     1681 gagcatcaac tgatacggcg tcggccaagc gtgctgcatg ttgtgcacca gcagctccgg
     1741 gcgaaatgcg aacaggaagc caaagcagag gatgcaggtt tcgctgatga cgtcgagtgc
     1801 cgacacgaag ccgttgaacg cggtactttc gcggacgccg acgacgttga gcgtcgcgag
     1861 cgccacgatg aaaccgagcg ttacgaggaa gtgcacccac ggatgaatcg agccggtgag
     1921 aaccggcgcc agctggctga gatagtccac gcaggtccac gcaaacagcg tgatgtcgat
     1981 ggtgaagtcg agcagcactg cccagcccgc gatgaatccg aagatgtcgc cgagcccgcg
     2041 caggacgaag tattggccgc cgcccgctac cgggtaggta gccgcgagct cggtgtacgc
     2101 gaggccgatg cagacgtaga cgatgccggc gaacaggaat gcgacgttgg acgcgccggc
     2161 ggccgccgcc aagaccagtc ccaagccgac gaaaatatcg gcaccgacgt cggaataacc
     2221 ccaagagaac gatccccagg gcgttaccgt acggcggagc tcgggtttcg tagacggcat
     2281 ttcggtcggc tagaaagcgt tccggtgaag gcggagccga ttgccgtcga gcgccacgac
     2341 gtcgaaccgg atagccgccg acggtgcgac gacttgcgcg taatcggcgg ccagacgccg
     2401 cagcgtcgcg cgcttgcgtg cgcccaccgc gcccagcgcc gggccgaagg ccgcgccgtc
     2461 gcggcgcttg acttccacga tgaccagcgt ggcaccgtcc aaacagacgg cgtcgatttc
     2521 gccgccgggc aggcggacgt tgcgcgcgat tacgcggtag ccgcacgact cgagaaaggc
     2581 gacggcgcgc tcttcgccgg cgcgtccttt ggcggcggtg ctcgcggcgt tc
//
LOCUS       DASUCJ010000128         1343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_857200, whole genome shotgun sequence.
ACCESSION   DASUCJ010000128 DASUCJ010000000
VERSION     DASUCJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857200"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="mreC"
                     /locus_tag="VFU90_01615"
     CDS             complement(<1..754)
                     /gene="mreC"
                     /locus_tag="VFU90_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5478493.1"
                     /translation="MIIVAALLALVQINAERTKSESPIATAASTSFGLAEAAISLAFG
                     GVRSAASAIAALPQLERDNARLRDDNRTLIAENARLHELAAQYAAEAGARVSVELFSG
                     IEARVVGFPPENESRSVTIDRGSSSGVRRDDGVVALGGVVGRVASVSPFTSTVVLITD
                     YTSRIPAVVRRGRWWGIARGNLTSVRMEFVPQDAPLKAGDTIVTGEGRSFHSGVPIGT
                     VVSVDRGDATLYQTAVVKPSVDLGALDRVVVVP"
     gene            complement(841..>1343)
                     /locus_tag="VFU90_01620"
     CDS             complement(841..>1343)
                     /locus_tag="VFU90_01620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU5478494.1"
                     /translation="PAIPGLRPAELAVRHAREKLTAALAQGEPELPIVAADTVVDRDG
                     AVFGKPQDAPDATRMLRLLSGREHRVHTAFALAIPGRDQWFEECSTAKVRFYPLGFDE
                     IAEYVATGEPLDKAGAYGIQGRAAALVEAIDGDFYTVMGFPLARFVRALRRLGFSLPT
                     AKPIAP"
BASE COUNT          231 a          470 c          445 g          197 t
ORIGIN      
        1 gcgggacgac gacaacgcga tcgagagcgc ccaggtcgac cgacggtttg acgaccgcgg
       61 tttgatagag ggtagcgtcg ccccgatcga cggaaaccac cgtgccgatc ggcacgccgg
      121 aatggaacga acggccttcg ccggtcacga tcgtgtcgcc ggccttcagc ggcgcgtctt
      181 gcggcacgaa ctccatgcga acgctcgtca aattcccgcg ggcgattccc caccaccggc
      241 cgcgccgcac gaccgccgga atgcggctcg tgtaatcggt gatcaacacc accgtgctgg
      301 taaacgggct caccgacgca acccggccga cgacgccgcc gagcgcgacc acgccgtcgt
      361 cgcgccgtac gcccgagctc gaaccgcggt cgatcgtcac cgagcgcgac tcgttttcgg
      421 gcggaaatcc gacgacgcgc gcttcgattc cggaaaacaa ctcgacgctg acccgcgcgc
      481 cggcttcggc cgcgtactga gccgccagct cgtgcagtcg ggcgttctcg gcgatcagcg
      541 tgcgattgtc gtcgcgcagc cgagcgttat cgcgttcgag ctgcggcaac gccgcgatcg
      601 ccgacgccgc cgaacgtacg ccgccgaatg ccagcgagat cgcggcttcg gccaatccga
      661 acgacgtcga cgccgccgtg gcaatcggac tctccgactt cgtacgctcc gcgttgattt
      721 gcacgagcgc caatagcgcc gcgacgatga tcacgctgat cagcgcaaaa agtcggcgtt
      781 cgtcgcgata cgtaaaaata ggcggcctct gcgtggatcc cggcgcggtg cggtgttggg
      841 ctacggtgcg atgggcttcg cggtcggcaa cgaaaatccc aaccgccgca aggcgcggac
      901 gaaccgggcg agggggaagc ccatcaccgt ataaaagtcg ccgtcgatcg cttccaccag
      961 cgccgcggcg cggccctgga tgccgtacgc cccggctttg tccagcggct ccccggtcgc
     1021 gacgtactcg gcgatctcgt cgaagccgag cggatagaag cgcactttgg ccgtcgagca
     1081 ctcttcgaac cactggtcgc ggcccgggat cgccagcgcg aacgccgtgt gaacgcggtg
     1141 ctcgcggccc gagagcaagc gcagcatccg ggtcgcgtcg ggggcatcct gcggctttcc
     1201 gaacacggcg ccgtcgcggt cgacgaccgt atcggcggct acgatcggca gctccggctc
     1261 gccctgcgcg agggcggcgg tcaacttttc acgggcgtga cgcaccgcca actcggcggg
     1321 gcgcaggccg ggaatcgccg gct
//
LOCUS       DASUCJ010000129         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_796135, whole genome shotgun sequence.
ACCESSION   DASUCJ010000129 DASUCJ010000000
VERSION     DASUCJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796135"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFU90_01625"
     CDS             complement(<1..774)
                     /locus_tag="VFU90_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478495.1"
                     /translation="MQRPRLSPPQMFNMSFGFLGIQFGWGLQLANMSAVYERLGARPD
                     QVPLLWLAAPMTGLLVQPVIGALSDRTWGPLGRRRPYFLTGAILASIALFLMPTSTTL
                     WMAATLLWILDASINISMEPFRAFVADKLPLSQRTQGFVMQSLMIGIGASLANALPYL
                     FAKFGVRGNTAGGIPTSVRYSFETGAVVFLLAVLWTVFTTTEFPPEDMAAFERARRGR
                     TGIGPLVAEIGDSLRKMPATMRQLAVVQLVTWLGLFCMWL"
     gene            835..>1728
                     /locus_tag="VFU90_01630"
     CDS             835..>1728
                     /locus_tag="VFU90_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5478496.1"
                     /translation="MKRAAIASVVWAGCAAAQAQLPAWTRGATCYEIFVRSFYDSNGD
                     GIGDLKGLIAKLDYINDGNPASRSSLGARCIWLMPVVASPSYHGYDATDYYRVNRDYG
                     TNEDFKQLVAAAHRRGIRVLVDMVLNHTSNQHPWFQSAMRDTASPYRAWYRWSKTKPN
                     EKGPWGQDVWYKSPVRDEYYYAVFWSGMPDLNYETPAVREEVKKIARFWLDDMGADGF
                     RLDAVPFLMEDHGKLAGSPGTHALLHEYEQYLRTVKPDVYTVGEVYDSLGAVLPYYPE
                     QLDSYFGFEMADSIIAAVRSGA"
BASE COUNT          334 a          542 c          587 g          265 t
ORIGIN      
        1 gagccacatg cagaagagac ccagccaggt caccagctgc accactgcca gttgtcgcat
       61 cgtcgccggc atcttgcgca gcgagtcgcc gatctccgcg acgagcggcc cgatccccgt
      121 gcgcccgcgt cgcgcccgct cgaacgcagc catgtcttcc ggaggaaact cagtcgtcgt
      181 gaacaccgtc cagagcacgg cgaggagaaa gacgaccgcg cccgtctcga acgagtagcg
      241 taccgacgtc ggaattccgc ccgccgtgtt gccgcggacg ccgaacttcg cgaagagata
      301 gggcaatgcg ttcgcgagac tcgccccgat tccgatcatg agcgactgca taacgaagcc
      361 ctgcgtccgc tgcgacagcg gcagcttgtc ggccacgaac gcccggaaag gctccatcga
      421 gatgttgatc gacgcgtcga gaatccagag cagcgtcgcg gccatccaca gcgtcgtcga
      481 cgtcggcatg aggaacagcg cgatggaggc gagaatcgcg ccggtgagga agtaggggcg
      541 gcgacgcccc agcggtcccc acgtgcgatc gctcagcgcg ccgataacgg gttgaacgag
      601 cagaccggtc atcggcgccg cgagccagag aaggggcacc tgatcggggc gggcgccgag
      661 tcgctcgtac accgccgaca tgttggcgag ctgcagtccc cagccgaatt ggatgccgag
      721 gaagccgaag ctcatattga acatctgcgg cgggctgagt cgtggcctct gcatgcgcga
      781 tccgcgaaag ggagacggtg aatgggcgcg acaagatgtg ggttggcgag ggccttgaaa
      841 agggcagcga tcgcctcggt cgtgtgggcc gggtgcgcgg cggcgcaggc gcagctcccg
      901 gcgtggacgc gcggcgcgac ctgctacgag atcttcgtcc gctccttcta cgacagcaac
      961 ggcgatggaa tcggcgacct gaaaggattg atcgcgaagc tcgactacat caacgacggt
     1021 aatccggcca gtcgcagcag cctcggcgcg cgctgcatct ggctgatgcc ggtcgtggcg
     1081 tcgccgagct atcacggcta cgacgccacg gactactatc gcgtgaaccg cgactacggc
     1141 accaacgagg acttcaagca gctcgtggcc gccgcgcatc gacgcggcat ccgtgtgctc
     1201 gtggacatgg tgctgaatca cacgtcgaac cagcacccgt ggtttcagtc ggcgatgcgg
     1261 gacacggctt cgccgtatcg cgcgtggtac cgctggtcga agacgaagcc taacgagaaa
     1321 gggccgtggg gccaggacgt gtggtacaag tcgccggtgc gcgacgagta ctactacgcc
     1381 gtgttctgga gcggcatgcc cgatctcaat tacgagacgc cggcggtgcg cgaggaggtg
     1441 aagaagatcg cgcggttctg gctcgacgac atgggcgccg acggcttccg tctcgacgcg
     1501 gtgccgttcc tcatggaaga ccatgggaag ctcgcgggca gcccggggac ccacgcgctg
     1561 ctgcacgagt acgagcagta tctccgcacg gtgaagccgg acgtctacac ggtgggcgag
     1621 gtatacgaca gcctcggcgc cgtgctgccg tattatccgg agcaactcga ctcatacttt
     1681 gggttcgaga tggcggacag catcatcgcc gcggtgcgtt ccggcgcc
//
LOCUS       DASUCJ010000130         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_918317, whole genome shotgun sequence.
ACCESSION   DASUCJ010000130 DASUCJ010000000
VERSION     DASUCJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918317"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFU90_01635"
     CDS             <1..506
                     /locus_tag="VFU90_01635"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478497.1"
                     /translation="AALGAPPGLLEQIAAFGDRRRHPSNAHVSIGYIALVASGSDGPA
                     ADQAAWYPVDELPAIAPRQRSIVEAAMQAIRARLDQSPIAFRLLPPTFTLSQLQEIYE
                     LLLGRRLHKASFRRALHASWLVEPTDEWRSEGRGRPAQLFRYAPRRRRGSRRGVRFEG
                     VSGSLGG"
     gene            complement(875..1165)
                     /locus_tag="VFU90_01640"
     CDS             complement(875..1165)
                     /locus_tag="VFU90_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478498.1"
                     /translation="MFLYLRELPGGGVVTIEATSGDGSSVSCRLAVERRSDSRRREGH
                     APPVIAEISAPTQSAAFRTLYEIAADNVAVARGLLVWQARRAKRGAAPDKAH"
     gene            complement(1221..2276)
                     /gene="ligD"
                     /locus_tag="VFU90_01645"
     CDS             complement(1221..2276)
                     /gene="ligD"
                     /locus_tag="VFU90_01645"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5478499.1"
                     /translation="MPARRPRTVRTRVPTRRLPRRPAPNAAIISALERIESAGGAAAV
                     EAAPHKSIEVSHLDKVFFPDAGVTKGDVMRYYVRVAKYILPAIADRPLVLKRTPEGVN
                     GELFFQQNAPDDVPDGVRVDNVAAQGEPQCRFVGGDLPTLLHLVQLGCISLDPWMSRI
                     GSLDHPDYSIIDLDPGPGASFKLVARVALWVREALDELGLRAVPKTSGSRGIHIAIPL
                     PRSTSYQDSIATAKAAAARVVDLHPRQATVERALENRPRGTVYVDCLQNSRGKSVAAA
                     YAVRAKPDATVSAPLTWDEVVESLDPREFTVRTMPARVEQHGDLWREGLKHGNSASVI
                     RAIATPGGKPRASAGKK"
     gene            complement(2417..2623)
                     /locus_tag="VFU90_01650"
     CDS             complement(2417..2623)
                     /locus_tag="VFU90_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5478500.1"
                     /translation="MSRITGTVKWFNDAKGYGFISREGGPDVFVHFSAIQGNGFKSLA
                     EGDQVEFEIVQGQKGPQAAEVQKL"
BASE COUNT          434 a          841 c          923 g          451 t
ORIGIN      
        1 gcgcagcgct tggcgcgccg cctggactgc tcgaacagat cgccgcgttc ggcgatcggc
       61 ggcggcatcc gtcgaacgcg cacgtgtcga tcgggtacat tgcgttggta gcgagcggga
      121 gcgacgggcc ggctgcagac caggcggcgt ggtatccggt ggatgagctg ccggcgatcg
      181 cgccgaggca gcgttcgatt gtggaggccg caatgcaggc gattcgcgcg cgtctcgatc
      241 aatctccgat cgccttccga ttgctcccgc cgacgttcac gttgagccaa ctgcaggaga
      301 tctacgagct gctcttgggc cgccgcttgc acaaggcgag cttccggcga gcgctccacg
      361 cgtcgtggtt ggtcgagccg acggacgaat ggcgcagcga gggtcgcggg cgtccggcac
      421 aacttttccg atatgcgcct cgcaggcggc gcgggagccg gcgcggggtg cggttcgagg
      481 gcgtgagcgg gtcactcggc ggttgacggt gctcgtgacc gaaccatgac gcgatcgcga
      541 ccggcgcgtg gttgccgccc gcggattttt agttgcaaaa tgtatctaag gacaaagcag
      601 tccaccgtag gatggagtcg cggcgcgttt cgctccgtga tggcgtccgc tcacacgccg
      661 tcggcatcgc ccgggtgtcc agggtcgtga cgacgcaggc gcggtcgctt cgctcggccg
      721 atgcatccgc gtccaacggc gcgccgaacg tccggcggcc ggtccaacgg cccaggcggc
      781 gatgacgccg cggcagcgcg gagcggcgcg gcggtcggat ctgaatgcct aaccgagagt
      841 ggcggtcccg ggcccggggc cgccacgcgt cgcgtcaatg ggccttgtcc ggtgcggcgc
      901 cgcgtttggc ccgccgcgcc tgccacacga ggagtccgcg cgcgacggcg acgttgtccg
      961 ctgcgatttc gtacaaggtt cgaaacgccg ccgactgggt cggagcgctg atttccgcta
     1021 tcacgggggg tgcatgtccc tcgcggcggc gcgagtcgct gcgccgttcc accgcgaggc
     1081 ggcacgacac ggacgaccca tcgccgctcg tggcctcgat cgtcaccacg ccgccgcccg
     1141 gcagctcacg caaatagaga aacacggcat ccgatcatcg aaggacacgt cctgcgccgg
     1201 catggcgact gcctaacgct ttacttttta ccggccgagg cgcgcggctt gccgcccggt
     1261 gtcgcaatag cacggatgac gctcgcagag ttcccatgct tgagtccctc gcgccagaga
     1321 tcgccgtgct gctcgacacg cgccggcatc gtgcgcacgg tgaactcgcg cgggtcgagc
     1381 gattccacga cctcatccca ggtgagcggc gccgaaaccg tggcatctgg tttcgcgcgc
     1441 accgcgtatg cggcagccac gctcttgccg cgtgagttct gtaaacagtc gacatacacc
     1501 gtaccgcgcg gccggttctc gagagcccgc tccacggttg cctgacgcgg gtgcagatcc
     1561 acgacgcgcg cggctgctgc tttcgcggtc gcgatcgaat cctggtacga cgtcgagcgc
     1621 ggcagtggga tcgcgatgtg gatgccgcgc gatcccgacg tcttggggac ggcgcggaga
     1681 ccaagctcat cgagcgcctc gcgaacccac agcgcgacgc gcgcgaccag cttgaacgag
     1741 gcgcccgggc cggggtcgag atcgatgatc gagtagtccg ggtgatccaa gctgccaatg
     1801 cgcgacatcc aggggtcgag tgagatgcag ccgagctgca cgagatgcag gagtgtcgga
     1861 agatcgccgc cgacaaagcg gcactgcggt tcgccctgag ccgcgacatt gtcgacgcga
     1921 acgccgtccg gcacatcgtc cggcgcgttt tgctgaaaga atagttcgcc gttcacgcct
     1981 tcgggtgttc gtttgaggac gagcggacgg tcggcaatgg cgggcaggat gtatttggcg
     2041 acgcgaacgt agtaccgcat cacgtcgcct ttggtcacac cggcatcggg gaagaagact
     2101 ttgtcgaggt gcgagacctc gatggacttg tggggagccg cttcgaccgc cgcggcgccg
     2161 ccggcgcttt ctatgcgttc gagggcggag atgatggcag cgttgggcgc cggccgtcga
     2221 ggcaggcgac gcgtcgggac gcgcgttcga accgtgcggg gacggcgagc aggcatgcgg
     2281 actccgacgg tgaagcggtg tgcacggcca tgcgtagcat cacgggatgc catgcacaag
     2341 atgtgctaac gctgctaaca aaaaagctcc ggcagcgcag ccggagcttt ttcgaatggg
     2401 gtgcgtgaga gcgggatcag agcttctgca cctcagctgc ttgggggccc ttctggcctt
     2461 gcacgatttc gaactcgacc tggtcgcctt cagccagcga cttgaagccg ttgccctgga
     2521 tcgcgctgaa atggacgaac acatcggggc cgccttcacg cgagatgaag ccgtaccctt
     2581 ttgcgtcgtt gaaccacttc acggtgcccg tgatgcgaga catggtacct tacctctccc
     2641 cctaaaggg
//
LOCUS       DASUCJ010000131         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1208804, whole genome shotgun sequence.
ACCESSION   DASUCJ010000131 DASUCJ010000000
VERSION     DASUCJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208804"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFU90_01655"
     CDS             <1..1255
                     /locus_tag="VFU90_01655"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5478501.1"
                     /translation="PADHNCSRPTTAIGLHWRRDRHGHVALAIPVAVFTRAPADSVSP
                     RAKQLHDRAIVVDSHGDTTQRLIFDKAFDIGKRNPNGNIDIPRMREGGLDALFFSIWV
                     PSEVTGPTAVKRALDQIDAVREAVRTHPHDLMLATTAADVRKAAADHKIAALMGMEGG
                     HMIDDDLRLLRVYAALGVRYMTLTHYKNNNWADSSTDEAAHNGLTAFGKDVVREMNRL
                     GVMVDISHVADKTFFDAIETTKAPMIASHSSSRVIANHARNMTDDMMRALARNGGVVM
                     INYHAAFLSEEFRVASEKKNGNVLGAIDAVSKKCGGNEACATMEGERIDHEAMMKGDL
                     PKVTWEKIIEHIDHAVKTAGVDHVGLGSDFDGATMPLGMEDASKLPKITDALLKKGYS
                     EADIEKILGGNILRVLEAVERVKGS"
BASE COUNT          274 a          423 c          436 g          198 t
ORIGIN      
        1 ccccgccgat cacaactgtt caagacccac gacggccatc ggcctacact ggcgacgtga
       61 tcgtcacgga cacgttgccc tggcgattcc ggttgccgtc tttacgcggg cgccggcaga
      121 ttcggtctcc ccgcgcgcga agcagctgca cgaccgcgcg atcgtcgtcg actcgcacgg
      181 cgacacgacg cagcggctga tcttcgacaa ggccttcgac atcggcaaac ggaacccgaa
      241 cggcaacatc gacatcccgc gcatgcgcga gggaggcctg gacgcgctgt tcttctcgat
      301 ctgggtgccg agcgaggtca ccggtccgac ggccgtgaag cgcgcgctcg atcagatcga
      361 cgccgtgcgc gaagcggtcc gcacgcatcc ccacgatctg atgctcgcga cgaccgccgc
      421 ggatgtgcga aaggcggccg cggatcacaa gatcgccgcg ctgatgggca tggaaggcgg
      481 ccacatgatc gacgacgatc tgcggctgct gcgcgtgtac gcggcgctcg gcgtccgcta
      541 catgacgctc acgcactaca agaacaacaa ctgggccgat tcctcgaccg acgaagcggc
      601 ccacaacggc ctcaccgcct tcggcaagga tgtcgtccgc gagatgaacc ggctcggcgt
      661 gatggtcgac atctcgcatg tggccgacaa gacgttcttc gatgcgatcg agacgaccaa
      721 ggcgccgatg atcgcgtcgc actcgtccag ccgcgtcatc gcgaaccacg cgcggaacat
      781 gaccgacgat atgatgcgcg ccctcgcgcg caacggtggc gtcgtgatga tcaactacca
      841 cgcggcgttc ctcagcgagg agtttcgggt cgccagcgaa aagaagaacg gcaacgtgtt
      901 gggggcgata gatgcggtgt cgaagaagtg cggcggcaac gaagcctgcg cgaccatgga
      961 gggtgaacgg atcgatcacg aggcgatgat gaaaggcgat ctcccgaagg tgacgtggga
     1021 aaagatcatc gagcacatcg accacgccgt gaagaccgcc ggcgtcgatc acgtcggcct
     1081 cggatccgac ttcgacggcg ccacgatgcc gctgggtatg gaagacgcgt cgaaactgcc
     1141 gaagatcacc gacgcgttgc tgaagaaggg ctactctgag gcggacatcg agaagatcct
     1201 cgggggcaac attctgcggg tgctggaggc ggtagaacgg gtgaaaggct catagttcag
     1261 cccgacgctg acggcggccg cactcggctg ggtccacatc ccgcagccgc cggtggaggt
     1321 ccaccgcgga c
//
LOCUS       DASUCJ010000132         7218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_459939, whole genome shotgun sequence.
ACCESSION   DASUCJ010000132 DASUCJ010000000
VERSION     DASUCJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459939"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFU90_01660"
     CDS             complement(<1..374)
                     /locus_tag="VFU90_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478502.1"
                     /translation="MTPATLRRALVALCIAAGACGRHPAPVARDARSPDDAHRRLPTG
                     VSLDPAGTATPVGQMPLAMVLSPDGTRLVLLLSGWREEGMQVVDRASGRVLQTLVQPS
                     AFVGLAFAPNGSALYASGADGDL"
     gene            complement(389..1156)
                     /locus_tag="VFU90_01665"
     CDS             complement(389..1156)
                     /locus_tag="VFU90_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478503.1"
                     /translation="MFFVANLGGDSAGGHDAFISRMHPDGSIDSLRFIDNGRNGARLD
                     APQGLALVGDTLWVVDRDAVRAFDALTGATGPSVSLAAQHPVALGGVAVGPDGALYVA
                     EPGLLDPAHPERRTGEGKRIFRVGRDHRVSVALASDSLHRPTDLVWDRRGDRFVIVSF
                     GTGNILAWHPGDAQPRTVGYNRAQMDGAAMLPDGRLLVTSWKDSSLTIRDGDRLTAIT
                     GFPLAAHIGVDTRRGRVAVPLPTRNRVDIWVVPPITQ"
     gene            2004..5219
                     /locus_tag="VFU90_01670"
     CDS             2004..5219
                     /locus_tag="VFU90_01670"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478504.1"
                     /translation="MKIARLSAAALGALLLHAAPSVAQATAYDTSMFHALSWREIGPY
                     RGGRSVAVAGSAARPLEFWMGTTGGGVFKTTDGGASWDPATDKYFGGTIGAIGVSESN
                     PDIVYVGAGEFDIRGNVSHGDGVWKTTDDGKTWESLGLTETRQISRVIVDPHNPDVVY
                     VGALGHVWAPNAERGVYKTTDGGKTWRKVLFRNDSTGVSDMAMDPNDPNTLYAAMWQA
                     GRKPWLLVSGGAGSGIFKTTDGGEHWTELTHNPGLPKGIIGDIGITVSPADPQRVYAI
                     VESDSGGVFRSDDGGATWTRTNQERKLRQRAWYYSRIFADPKDSNTVYVVNVSFFRST
                     DGGKTFNPIAVPHGDNHDLWIAPNDPKRMIESNDGGATVTFDGGKAWSDEDYATAQFY
                     HVTTTTDFPYKVCGAQQDNSTLCGPSRWPGGIPISEWQEVGGGESGYIQVRPDSTNIV
                     YAGSYAYLSRMDLSNGQRRDIIPWPDNPMGHSAEDLKYRFQWTFPILISPHDPNTLYL
                     GSQVLFRTHNGGQSWEIASPDLTRHDPATLGPSGGPITKDQTSVEYYATIFAIAESPV
                     VKGVIWTGSDDGLIYVTRDNGKTWSNVTPKDLPPFTRISIIDASHFGAGTAYVAANRY
                     QLDDDAPYLYKTSDYGKTWTRIDAGIDRTQFTRTIREDPERRGLLYVGTERGVWVSFD
                     DGSHWQSLQRNLPKVPVHDLEVTQGDLVAATHGRSFYILDDVSALRQLTPEIAQAPMH
                     LFKPRAAYRVNWGGGFGGGGGREHPVGKNPPNGAVVYYTLSSPNQKVTLDFLDAKGAV
                     IQSFSSQLDSAGLADSVRADSIKKANKGKEPAAEGGFRRPTRPRVSNTAGLNMFAWDL
                     RYPDAVGFTNLIMWAGSTRGPIAVPGTYSVRLTVNGKSQTQSFVLKKDPRVHATQADF
                     EAQFALLMKVRDRLSAADNAVRTIRNLEWQMDQHGSSAPSGFADAAGALKTKLDTIEA
                     AIYQVHNRASEDPLNFPIRLNNKIAALGGSIASADARPTAQDYAVYTLLSGQLDHELA
                     ELKTTLASGLPPLNAMLRGAGQKELVPSTSEPPQKEVAEIE"
     gene            complement(5254..>7218)
                     /locus_tag="VFU90_01675"
     CDS             complement(5254..>7218)
                     /locus_tag="VFU90_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5478505.1"
                     /translation="PHVVGRRIRLAGRSYTIIGVAPAGYEGTFPGVVPALFLPLRMVN
                     QLESTSVDELTDRDSHNLFLKARLAPGVSIARLRAMAGAFTAEMQRRFPSYWPGSAST
                     LVIPETEIVVNPLLDNIVVPAAAALMAVVALVLLVACANLASFLLAQARDRQREIAIR
                     LALGATRWSLVRQLLVETVVLAAVGGLAGVFLSRLALGALLHADLPLPLPITVDVHLD
                     ARILGFAVLAATAAAMFVGVLPALQATRPNIVDVIKNENTGGGPPRRVTMRSALVVGQ
                     IAVSLVLLVTAGLFLRSLESREHIDPGFGRAPAGVVWFGFPGDRKTGPQEQRMVDDIE
                     GRARRIAGVNAVGVTDNLLLSIITTQTALVNIAGFQPPKGEPGFTIDYAAADSGFFSA
                     AGVRLLRGRGIQASDDSASPLIAVVNEVMAKEFWPGRDPIGQTFRRDSNVYRVVGVTQ
                     TTKVRSLGEEPRPFMFLSFAQSPNPDAMLVARTTPGRDESLTPELIAMLHDVDPTIPV
                     LQGTTMARHIGAVMLPTRLGAIAFGVFAALALALAMIGVYGVVSYAVARRAREVGIRM
                     ALGAEPNEVVRLLMREGLVMVGGGGVLGLVLALAVTRVLASLLSGVTAVDPLTFVVAP
                     VVLVTVGIMAALLPALRSARTDPVRVLRAE"
BASE COUNT         1339 a         2449 c         2387 g         1043 t
ORIGIN      
        1 aggtcgccgt cggcgcccga agcgtagagc gcgctaccgt taggcgcgaa cgcgagaccc
       61 acgaacgcgg acggctggac gagcgtctgc agcacgcggc cgctcgcgcg atccaccacc
      121 tgcatcccct cctcgcgcca ccccgagagc agcagcacga gccgcgtgcc gtccggcgaa
      181 agcaccatgg ccagcggcat ctgccccacc ggcgtcgcgg tgccggccgg atcgagggag
      241 acgcccgtgg gcagacggcg gtgcgcgtcg tccggcgacc gcgcatcccg cgcgaccgga
      301 gccggatgcc ggccgcacgc gccggcggcg atgcacagcg cgacgagcgc gcgacgaagc
      361 gttgccggtg tcatcgatcg cgcacgggtc attgcgtgat gggcggcacc acccagatgt
      421 cgacgcggtt tctggtcggc agcggcaccg ccacgcggcc gcgccgcgtg tcgacgccga
      481 tgtgcgcggc taacggaaac ccggtgatcg ccgtcaggcg gtcgccatcc cgaatggtga
      541 gcgaggaatc cttccagctc gtcacgagca gacggccatc gggcagcatg gccgcgccat
      601 ccatctgcgc gcggttgtat cccaccgtgc gcggctgcgc gtccccggga tgccaggcga
      661 gaatgttgcc ggtgccgaac gacacgatca cgaaccggtc gccgcgccgg tcccagacga
      721 ggtcggtggg ccggtgcagc gagtcgctgg cgagcgccac cgagacgcgg tgatcgcgtc
      781 cgacgcgaaa gatgcgcttc ccctcgccgg tgcggcgttc cgggtgcgcc gggtcgagca
      841 ggccgggctc ggcgacgtag agcgcgccgt cgggcccgac cgcgacgccg cctaacgcaa
      901 ccggatgctg cgcggcgagg ctcaccgagg gacccgtcgc cccggtaagc gcatcgaacg
      961 cccgcaccgc atcgcggtcc accacccaca gcgtgtcgcc cacgagcgcc aagccctgcg
     1021 gcgcatcgag ccgcgccccg tttcgtccgt tgtcgatgaa ccgcagcgaa tcgatgctgc
     1081 cgtccggatg catccggctg atgaacgcgt cgtgcccgcc cgccgaatcg ccgcccagat
     1141 tcgcgacaaa aaacatgtcg gccgcggaat cgtagcgcgc ggcaaccggt cgctcgaaac
     1201 ctcgaacgct cgcggcatgc gtgggcggga caaacggctt ggccggcggc tgtgcgttct
     1261 ttgccgtgtc cggggcacca caccctgcca acacgaaggc gagaacaaaa attcggcgca
     1321 cgctgatgcg gaaaaagggc aagagaaagg cggacacaac gcggacacaa gagacaatca
     1381 cggaccgttc atgccgcagt cgaaggtgcg tctcgcgaga gggtgctcag gctcgagtcg
     1441 cctgccgggc cgccgcgacc atgatagaaa ggataacaag cgtgaaactg aattgacaat
     1501 gaaagagatc aggctgcgcg ggagtaaggg tttgcctaac ggatagttga gttttctgtg
     1561 aaagagaaac tgatcaacac cctgatgcct gcgccgagca acactcgcac ccgcgcggca
     1621 cgatgtcttt cgtcatccgt tcgatttcgc tttgtaactt ttgattatga gcgaggggac
     1681 ggcaaaggca catgaacttt gaagggtcga cggatctcac gaaagaccgc ccggctcgac
     1741 cgacctcgcg tgatgccagg agaccctaac ggttcgcgcc ctcccccgta aggtccgcga
     1801 tttgtccgtc tgtagtccgc gccctgtccg cctgtaaatc tccgccttgt ccgcctgtga
     1861 gtgtccgcct tgtccgtctg taagtgtccg ccttgtccgc ctttatatca gcggattgtc
     1921 gcccgtaatc cgccttcagt cttccctcgc cgcggtccct cctctacaat cttggccacg
     1981 aactgaccaa gtcgaggaca ccagtgaaaa tcgcacgcct gtcggcggcg gcgctcggcg
     2041 cgctgctcct tcacgctgcg ccgtcggtcg cccaggccac ggcatacgac acgtccatgt
     2101 tccacgcgct atcgtggcgc gagatcggac cgtatcgcgg cggacgctcc gtcgcggtcg
     2161 cgggctcggc ggcgcgtccg ctcgagttct ggatgggcac caccggcggc ggcgtgttca
     2221 agaccacgga tggcggcgcg agctgggatc cggcgaccga caagtatttc ggcgggacga
     2281 tcggcgccat cggcgtgagc gaatcgaatc ccgacatcgt gtacgtcggc gcgggcgagt
     2341 tcgacatccg cggcaacgtg tcgcacggcg acggcgtctg gaagaccacc gacgacggca
     2401 agacctggga gtcgctcggc ctaacggaga cgagacagat ctcgcgcgtc atcgtcgatc
     2461 cgcacaatcc ggatgtcgtg tacgtcggcg cgttaggcca cgtgtgggcg cctaacgccg
     2521 agcgcggcgt gtacaagacc accgacggcg gcaagacctg gcgcaaggtg ctgttccgca
     2581 atgattcgac cggcgtcagc gacatggcga tggatccgaa cgatccgaac acgctgtacg
     2641 ccgccatgtg gcaggcggga cgcaaaccgt ggctcctggt cagcggcggc gccggcagcg
     2701 ggatcttcaa gacgaccgat ggcggcgagc actggaccga gctcacgcac aatcccgggc
     2761 tgcccaaggg catcatcggc gacatcggga tcactgtctc gccggccgat ccgcagcggg
     2821 tctacgcgat cgtcgaatcc gactcgggcg gcgtcttccg ctccgacgac ggcggcgcca
     2881 cgtggacgcg cacgaaccag gagcggaagc tgcggcagcg cgcctggtat tactcgcgga
     2941 tcttcgccga cccgaaggac agcaacaccg tgtacgtggt caacgtgtcg ttcttcagat
     3001 ccaccgatgg cggcaagacg ttcaacccga tcgccgtgcc gcacggggac aatcacgact
     3061 tgtggattgc gcctaacgat cccaagcgga tgatcgaatc gaacgatggc ggggcgaccg
     3121 tcaccttcga cggcgggaag gcgtggagcg atgaggacta cgccaccgcg caattctacc
     3181 acgtcaccac gaccaccgat ttcccgtaca aggtctgcgg tgcgcagcag gacaactcga
     3241 cgctctgcgg accgagccgc tggccgggcg gcattccaat cagcgagtgg caggaagtgg
     3301 gcggcggcga gtcgggctac atccaggtgc ggcccgacag cacgaacatc gtctacgcag
     3361 gcagctatgc gtatctgtcg cggatggacc tgtctaacgg acagcggcgc gacatcatcc
     3421 cgtggcccga caatcccatg ggccactcgg ccgaggatct gaagtaccgg ttccagtgga
     3481 ccttcccgat tctcatctcg ccgcacgatc cgaacacgct gtacctggga tcgcaggtgc
     3541 tgttccgcac gcacaacggc ggccagagct gggaaatcgc gagccccgac ctcacccggc
     3601 acgacccggc aacgttaggc ccgtccggcg gtccgatcac caaggaccag acgtcggtcg
     3661 agtactacgc cacgatcttc gccatcgccg agtcgcccgt ggtgaagggc gtcatctgga
     3721 cgggatcgga cgatggattg atctacgtca cgcgcgacaa cgggaagacg tggtccaacg
     3781 tgacgccgaa ggacctgccg ccgttcacgc gcatctcgat catcgatgcg tcgcatttcg
     3841 gcgcgggcac ggcgtacgtc gcggccaacc gctaccagct ggacgatgac gcgccctacc
     3901 tctacaagac atcggactac ggcaagacgt ggacgcgcat cgatgcgggc atcgaccgca
     3961 cgcagttcac gcgcacgatt cgcgaggatc cggagcgccg cggcttgctg tacgtcggca
     4021 ccgagcgcgg cgtatgggtg agcttcgacg acgggtcgca ctggcagtcg ttgcaacgca
     4081 atctgccgaa ggtgccggtg cacgacctgg aggtcacaca gggcgatctg gtcgccgcga
     4141 cgcacgggcg gtcgttctac atcctcgatg atgtgtcggc gctgcggcag ctgacgccgg
     4201 aaatcgcgca ggcgcccatg catctgttca agccgcgcgc ggcgtatcgc gtgaactggg
     4261 gcggcggatt cggcgggggc ggcggacgcg agcaccccgt cggcaagaat ccgccgaacg
     4321 gcgccgtggt gtactacacg ctctcgtcgc ctaaccagaa ggtgacgctc gacttcctcg
     4381 atgcgaaggg cgcggtgatc cagtcgttct cgagccaact ggacagcgcc ggtctcgccg
     4441 acagcgtgcg cgccgacagc atcaagaaag cgaacaaggg caaggagccg gccgcagagg
     4501 gagggttccg gcgtccgacg cgtccgcgcg tgtccaacac ggcggggctc aacatgttcg
     4561 cgtgggacct ccgctatccc gacgcggtcg gattcacgaa cctcatcatg tgggccggct
     4621 cgacccgcgg gccgatcgcc gtgccgggca cgtactcggt ccgcctaacg gtgaacggca
     4681 agtcgcagac gcagtcgttc gtgctcaaga aggatccgcg cgtccacgcc acgcaagcgg
     4741 acttcgaagc gcagttcgcc ctcctgatga aagtgcgcga ccggctctcg gccgccgaca
     4801 atgcggtgcg cacgatccgc aatctcgagt ggcagatgga tcaacacggg tcgtccgcgc
     4861 cgtccggctt cgccgatgcg gcgggcgccc tcaagacgaa gctcgacacg atcgaggcgg
     4921 cgatctacca ggtgcacaac cgcgcgagcg aggatccgct caacttcccg ataaggctga
     4981 acaacaagat cgcagcgtta ggcggctcga tcgccagcgc cgacgccagg ccgacggcgc
     5041 aggactacgc cgtgtacaca ctgctgtccg gccagctcga tcacgagctc gccgagctca
     5101 agacgacgct ggcgtccggg cttccgccgc tcaacgcgat gctgcgcggc gcgggccaga
     5161 aggagctcgt gccgtccacg tccgagccgc cgcagaagga ggtagccgaa atcgagtgag
     5221 agagaaaggg cggacaccgc gcggtgtccg ccttcactca gcgcgcaaca cgcgcaccgg
     5281 atcggtgcgc gccgaccgca gtgccggcag cagcgccgcc atgatgccca ccgtcacgag
     5341 cacgaccggc gcgacgacga acgtcagcgg atccacggcc gtcacgcccg acaagagact
     5401 cgcgaggacg cgcgtcaccg cgagggcgag caccagacca agcacgccgc cgccgccgac
     5461 catcacgagt ccctcccgca tgaggagccg gacgacttcg ttaggctccg cacccagcgc
     5521 catccgaatg cccacctcgc gcgcgcgccg cgccaccgcg tagctcacga cgccgtagac
     5581 gccgatcatc gccagcgcca gcgcgagcgc ggcaaacacg ccgaacgcaa tggcgccgag
     5641 ccgcgtcggc agcatgacgg cgccgatgtg tcgcgccatc gtcgttccct gcagcaccgg
     5701 tatcgtcgga tccacgtcgt gcagcatggc gatcagctcc ggcgtcagcg actcatcccg
     5761 tcccggagtc gtgcgcgcga cgagcatcgc atccgggttc gggctctgcg cgaacgacag
     5821 gaacatgaac ggtcgcggct cttcgccgag cgagcgcacc ttggtggttt gcgtgacgcc
     5881 gacgactcga tacacgttgg aatcgcggcg gaacgtctgc ccaatcggat ccctgcccgg
     5941 ccagaactcc ttcgccatca cctcgttcac cacggcgatg agcggcgatg ccgaatcgtc
     6001 agacgcttga atgccgcggc cgcgcagaag cctaacgcca gcggcggaga agaaacccga
     6061 gtcggcggcc gcatagtcga tcgtaaaccc cggctcgcct ttgggcggct gaaagccggc
     6121 gatgttgacg agagcggtct gcgtggtgat gatggacagc agcaggttgt ccgtcacgcc
     6181 gacggcgttc acgccggcaa tccggcgggc gcgcccttcg atgtcgtcca ccatccgctg
     6241 ctcctgcggc cccgtttttc gatcgcccgg gaacccgaac cagacgacgc cggccggcgc
     6301 gcgtccgaag ccgggatcga tgtgctcgcg cgactcgaga cttctcaaaa agagacccgc
     6361 cgtgacgagc agcacgagcg acaccgcgat ctggccgacc acgagcgcgc tccgcatcgt
     6421 gacgcggcgc ggcggaccgc cgccggtgtt ctcgttcttg atgacgtcga cgatgttggg
     6481 ccgtgtggcc tggagcgccg gcagcacgcc cacgaacatc gccgcggcgg tggcggccaa
     6541 cacagcgaag ccgaggatcc gcgcgtccag gtgcacgtcg acggtgatcg gaagcggcag
     6601 cggcaggtcc gcgtgcaaga gcgcgcctaa cgcaagccgc gagaggaaga cgccggccag
     6661 accgccgacc gccgccagca cgacagtttc cacgagcagt tgccggacga gcgaccagcg
     6721 ggtcgcgccg agggccagcc gaatcgcgat ctcccgttgg cgatcgcgcg cctgcgccag
     6781 caggaagctc gcgaggttcg cgcacgccac caacaacacc agcgcgacca cggccatgag
     6841 cgcagcggcc gccggtacca caatgttgtc gagaagcggg ttgacgacga tctcggtttc
     6901 cggaatcacg agggtcgacg cagagcccgg ccagtaggaa ggaaaccgcc gctgcatttc
     6961 ggccgtgaag gcgccggcca tcgctcgcag ccgcgctatc gatacgccgg gtgcgagtcg
     7021 agccttcagg aagaggttgt ggctgtcgcg gtccgttagc tcatcgacgg aggtcgactc
     7081 gagctggttg accatccgga gcggcaggaa cagcgccggc acgacaccgg gaaaggtgcc
     7141 ctcgtatccg gcgggggcga cgccgatgat ggtgtagctc cgtcccgcga gccggatccg
     7201 tcgcccaacg acgtgggg
//
LOCUS       DASUCJ010000133         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_872490, whole genome shotgun sequence.
ACCESSION   DASUCJ010000133 DASUCJ010000000
VERSION     DASUCJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872490"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..>1706
                     /locus_tag="VFU90_01680"
     CDS             77..>1706
                     /locus_tag="VFU90_01680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5478506.1"
                     /translation="MRAFAIITALAVMAWPAAADAQSAPTAGVADTSPFRALALPTPN
                     EFRTGSGRPGPRYWQQRVDYRIAATLDPARNAVRGRETIHYTNRSPDTLPYLWLYLEQ
                     NICAPNSVTNLLNQPPLAFLGSSFDFSCQGFNGGITLDSVSVAGREVRGADTAIYGTT
                     MRVDLPTPLRPGASIDVDFAWHFGVPQQGAGRMGHDGPLYEIAQWYPRVAVYDDVRGW
                     NHEPYIGAGEFYLEYGSFDVALTVPATYIVAATGRLLNPERVLTAAQRSRLTSARHSP
                     NPVAIITKDEAGNAGRTRPTARGTLTWHFLADSVRDFAFAAGPNFRWDATNYDGILIE
                     TLYRPTADKWEEAIRMAKSAIQYFSEQWYRYPYPHATTVEGPIEGMEYPMLTFVPNSP
                     TREDQQWALSHEFGHEWFPMIVGSNERLYPWMDEGFNTFIDLGGAARYFAGSPYGDTI
                     EVHPLHLYATHAVPGNEQPLIEKPVEVRDLFWGGYQKPALMMQMLRYEVLGKDRFDAA
                     FRAYIRAWAFKHPTPADFFRVMRDQSGMELDWFWR"
BASE COUNT          296 a          582 c          541 g          287 t
ORIGIN      
        1 cgatcgagga gtacttcggg ggcgaggagc acgatctgga ttggaggctc gtggtgcggt
       61 gaaggtgatt ggagccgtga gagccttcgc catcatcacc gcgctcgccg tcatggcctg
      121 gccggccgcg gcggacgcgc aatcggcgcc gaccgcgggc gttgccgaca cgtcgccctt
      181 tcgcgcgctc gcgctgccga cacctaacga gtttcgcacc ggctctggcc gtccgggacc
      241 gcgctactgg cagcagcgcg tggactatcg gatcgccgcc acgctcgatc cggcacgcaa
      301 cgcggtgcgg ggtcgcgaga cgatccacta cacgaaccgg tcccccgaca cccttcctta
      361 cctctggctg tatctcgagc agaacatctg cgcgcccaac agcgttacca atctgctcaa
      421 tcagccgcca ctcgcctttc tcggctcgtc gttcgacttc tcctgtcagg gattcaacgg
      481 cgggatcacc ctggattccg tgtcggtggc ggggcgcgag gtgcggggcg cggacacggc
      541 gatctatggc acgaccatgc gcgtcgatct gccgacgcca ctgcgacccg gtgcgtcgat
      601 cgacgtcgac ttcgcctggc acttcggcgt cccgcaacag ggcgccgggc gcatggggca
      661 cgacgggccg ctctacgaga tcgcgcagtg gtatccacgc gtcgccgtgt acgacgacgt
      721 gcggggctgg aatcacgaac cctacatcgg cgccggcgag ttctacctcg agtacggcag
      781 cttcgatgtc gcgctcaccg tgccggcgac atacatcgtc gccgcgacgg gacgtctctt
      841 gaatcccgag cgcgtgctca ccgcggcgca gcgctcacgc ctcacgagtg cgcgccactc
      901 gcccaacccc gtggcgatca tcacgaagga cgaagcgggc aacgccggcc ggacgcgtcc
      961 gacggcgcgc ggtaccctca cctggcactt cttggccgac agcgtacgcg acttcgcctt
     1021 tgcggccggc ccgaacttcc gctgggacgc caccaactac gacggcattc tgatcgagac
     1081 gctctaccgt cccacagccg acaaatggga agaagcaatt cgcatggcga agtcggcaat
     1141 ccagtacttc agcgaacagt ggtaccggta tccctatccg cacgccacca cggtcgaggg
     1201 gccgatcgaa ggcatggagt acccgatgct caccttcgtg ccgaacagtc ccacgcgcga
     1261 agatcagcag tgggcgctgt cgcacgagtt cggccacgag tggttcccga tgatcgtcgg
     1321 ctcgaatgaa cggctctacc cttggatgga cgaaggattc aacaccttca tcgacctcgg
     1381 cggcgcggcg cgatacttcg cgggctcgcc ctacggcgac acgatcgagg tgcacccgct
     1441 gcacctgtac gcgacccacg cggtgcccgg gaatgagcag ccactcatcg aaaagccggt
     1501 cgaggtgcgg gatctgttct ggggcggcta tcagaagccg gcgctcatga tgcagatgct
     1561 gcgctacgag gtactcggca aggatcgctt cgacgccgcg ttccgcgcgt acatccgcgc
     1621 gtgggcgttc aaacatccaa cgccggcgga cttcttccgc gtcatgcggg accaatcggg
     1681 catggagctc gactggttct ggcgtg
//
LOCUS       DASUCJ010000134         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_230709, whole genome shotgun sequence.
ACCESSION   DASUCJ010000134 DASUCJ010000000
VERSION     DASUCJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230709"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFU90_01685"
     CDS             <1..441
                     /locus_tag="VFU90_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5478507.1"
                     /translation="MTVDDWRQVLNVNLSGAFQMTKAVLEHMIERGSGRIVMISSVIG
                     ETGNVGQANYAASKAGLFGFTKSLALEMAQRGITVNCVAPGFVATEMVEAIPAAALER
                     VIEKIPQRRLGRPEEVARVVRFLLEDEASYITGAVFSVNGGLDM"
     gene            483..1238
                     /locus_tag="VFU90_01690"
     CDS             483..1238
                     /locus_tag="VFU90_01690"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEU5478508.1"
                     /translation="MNSLPLEGRVAIVTGGSRGIGGAISRLLADDGATVAVLGLPIDR
                     ERTETLRVSLNGSAQRLHFYQGDVAEFDECRRAVARVLDQHGRIDYLINNAGITRDHT
                     VRKMTVDEWQAVLQVNLSGPFFMIKAVLDTMTAQEFGRIVNISSVVGESGNFGQANYA
                     AAKAGLIGLTKTVALEVAKKRITVNAVAPGFIETDMTKAMPADAIETAISQTPKRRLG
                     QPEEVAHVVRFLVDDKAGYITGAVYNVNGGWYM"
     gene            1235..>1726
                     /locus_tag="VFU90_01695"
     CDS             1235..>1726
                     /locus_tag="VFU90_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478509.1"
                     /translation="MSASPDQLPNGPMSADDLSYGIDVTGLDPASLNDALRAVIADAM
                     ADPLRMSTWMSGFALAEQNVGLNMLRRFGGQEPMPLLGRDAPADKRFAEPEWSSNPVL
                     AGIVEDYQMRAQAAFQLVDSARLPAATRRKARFAMQLMVDAFAPSNVPWLNPGVVKEA
                     MNTG"
BASE COUNT          332 a          534 c          584 g          276 t
ORIGIN      
        1 atgacggtcg acgactggcg gcaggtgctc aacgtgaatt tgtccggcgc ctttcagatg
       61 accaaagccg tcctcgagca catgatcgaa cgcggatccg ggcgtatcgt gatgatcagc
      121 tcggtgatcg gcgaaaccgg aaacgtcgga caggccaact atgccgcctc caaggccggc
      181 ctgttcggct tcaccaagag tctggcgctg gagatggcgc agcgcggcat cacggtcaac
      241 tgcgtcgcgc ccgggttcgt cgcaaccgaa atggtcgagg ctatcccggc ggccgcgctc
      301 gaacgggtca tcgagaaaat tccgcagcgc cggctcggac gccccgaaga agtggcccgg
      361 gtggtgcgct ttctgctgga ggacgaggcg agctacatta ccggggcggt attctcggtc
      421 aacggcgggc tcgatatgta ggggtgaagg taaacccagg gcgtgctatg taaatctagc
      481 gcgtgaacag cttaccactc gaaggccggg tcgcgatcgt caccggcgga tctcgcggaa
      541 tcggcggtgc gattagccgc ctgttggccg acgacggtgc gaccgtcgcc gtgctgggtc
      601 tcccgatcga ccgggagcga accgaaacgc tgcgcgtatc gctcaacgga agcgcgcagc
      661 gactgcactt ttatcaaggc gacgtcgccg agttcgacga gtgccgccgg gcggtcgcgc
      721 gcgtgctcga tcagcacggc cgaatcgatt acttgatcaa caacgccggc atcacgcgcg
      781 accatacggt gcgtaagatg acggtcgacg agtggcaagc ggtcctgcag gtcaatctgt
      841 cgggaccgtt tttcatgatc aaggcggtgc tcgatacgat gaccgcccaa gagttcgggc
      901 ggatcgtgaa catcagctcg gtcgtgggcg agagcggaaa cttcggacaa gccaactacg
      961 cggccgccaa ggccgggctg atcggtctga ccaaaacggt ggccctcgag gtcgccaaga
     1021 agcgcatcac cgtcaacgcg gtcgcgccgg gattcatcga aacggatatg accaaggcga
     1081 tgccggccga tgcgatcgaa acggcaatct cgcagacgcc gaagcggcgg ctcggacagc
     1141 cggaggaggt cgcccacgtg gtgcgcttcc tggtggacga caaggccggc tacatcaccg
     1201 gcgccgtgta taacgtcaac ggcggctggt acatgtgagc gcgtccccgg accaattgcc
     1261 caacgggccg atgtcggccg acgatctcag ctacggcatc gacgtcaccg gactcgaccc
     1321 cgcttcgctc aacgacgcgt tgcgcgccgt catcgccgac gcgatggcgg acccgctgcg
     1381 gatgagcact tggatgtcgg gtttcgcact ggccgaacaa aacgtcggcc tcaacatgct
     1441 gcggcggttc ggcggccagg agccgatgcc gctgctcggc cgcgacgcgc ccgccgacaa
     1501 gcgattcgcc gagccggagt ggtcgagcaa tccggtgctg gccggcatcg tggaagacta
     1561 tcaaatgcgc gcgcaggcgg cgtttcaact ggtcgactcg gcgcggctgc cggcggccac
     1621 gcggcgcaag gcgcgcttcg cgatgcagct gatggtcgac gcgttcgcgc cgagcaacgt
     1681 gccgtggctc aaccccggcg tcgtcaagga agcgatgaac acgggc
//
LOCUS       DASUCJ010000135         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_291744, whole genome shotgun sequence.
ACCESSION   DASUCJ010000135 DASUCJ010000000
VERSION     DASUCJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291744"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFU90_01700"
     CDS             complement(<1..1230)
                     /locus_tag="VFU90_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5478510.1"
                     /translation="MQRGSEAGAIVGGYHGDPFSYLGPHPLADGRTLVRVFQPGARTV
                     ALTDFAGQAVASEPTECSSGLFEIAVTGPLPHPYRLRVAYDGGTAEIVDPYQFGPWLG
                     EMDLYLLREGTHRRAYDRLGAHLVSMETVEGVVFAVWAPNASRVSVVGDFNQWDERRH
                     PMRLHPGAGIWEIFIPGLGEGERYKYAIRTASGGRMPMKADPFAFRAERPPATASIVC
                     RNDRHQWHDQSWIDERGRRNLYGAPMTIYEVHLGSWRRRPDEGNRSLSYRELASTLVP
                     YVRDMGFTHIEMLPIAEFPFDGSWGYQPTGLFAPTSRFGTPDEFQAFIDACHQAEIGV
                     LLDWVPGHFPNDPHGLADFDGTKLYEHADPRRGIHRDWNTLVYNYGRTEVAEYLQTNA
                     LFWFDRFRVDGLRVDAVA"
     gene            complement(1217..>2278)
                     /locus_tag="VFU90_01705"
     CDS             complement(1217..>2278)
                     /locus_tag="VFU90_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478511.1"
                     /translation="IMKFYRRVAVGTHPEIELGAFLTGTDAYANTPTLFGSIHLVDAA
                     GNDTALGVVHALIRNQGDGWTYTLAYLDRFLQDIRVAPAEPQPLPTEHHAAYLVYIEQ
                     LARRTAEFHRVLYVEEGDPAFVVERAGPERMRDWGEETARRVEAALDALHAVRGRLSA
                     DAIAALDELAQQRGAVLAQIRSAAAGNTDVLLSRCHGDYHLGQTLVVQNDVSIVDFEG
                     PPQLSLADRRAKHSIVRDVASMMRSFDYAAWTALDHATANRPDYRNELSPAVIEWRDQ
                     VSTRFLDTYVATMADCPLWPRDAAIARNLLSLFLIEKAALEIDYELGNRPGWAGVPLA
                     GLRTLLLGRQIPERSDAAG"
BASE COUNT          409 a          721 c          790 g          358 t
ORIGIN      
        1 ggcgacggca tcgacccgca gcccgtcgac gcggaagcgg tcgaaccaga acagcgcatt
       61 ggtctggaga tattcggcga cttccgtgcg gccgtaattg tagaccagcg tgttccagtc
      121 gcgatggatg ccgcggcgcg gatcggcgtg ctcgtaaagc ttggtgccgt cgaaatcggc
      181 gagcccgtgc ggatcgttcg ggaaatggcc cggcacccag tcgagcagca cgccgatctc
      241 ggcctgatgg caggcgtcga tgaacgcttg gaactcgtcg ggcgtcccga agcggctggt
      301 cggcgcgaac agccccgtcg gctgataccc ccaggagccg tcgaacggaa attcggcgat
      361 cggcagcatt tcgatatggg tgaagcccat gtcccgtaca tagggaacga gcgtcgacgc
      421 cagctcccga tagctgagcg agcgattgcc ctcgtcgggg cggcggcgcc acgagccgag
      481 atgcacctcg tagatcgtca tcggcgcgcc gtagaggttg cgccgcccgc gctcatcgat
      541 ccaggactgg tcgtgccatt gatgccggtc gttgcggcag acgatcgagg ccgtggcggg
      601 cgggcgctcg gcgcggaacg cgaagggatc ggccttcatc ggcatgcggc cgccactggc
      661 ggtgcggatc gcgtatttgt agcgctcgcc ttcgccgagc cccggaatga agatttccca
      721 aattccggcg ccgggatgaa ggcgcatcgg atgacgccgc tcgtcccatt gattgaaatc
      781 gccgacgacg ctgacgcgcg aggcgttggg tgcccacacc gcgaacacca cgccctcgac
      841 cgtctccatg ctgacgagat gcgcgccgag ccggtcatag gcgcggcgat gggtcccttc
      901 gcgcaacagg tagaggtcca tttcgccgag ccacggaccg aactgatagg gatcgacgat
      961 ctctgccgtg ccgccatcgt aagcaacgcg cagacgatag ggatgcggca aaggccccgt
     1021 cacggcgatc tcgaaaagcc cgctggagca ttcggtcggc tccgacgcca cggcttggcc
     1081 ggcgaaatcg gtcaacgcaa ccgtgcgcgc gcccggctgg aacacgcgca cgagggtgcg
     1141 gccgtcggca agaggatgtg gacccagata ggagaacggg tcgccgtgat atccgccgac
     1201 aatcgcgccg gcttcgctac cccgctgcat cggacctttc gggtatctgc cgccccagca
     1261 gtaaagtccg gagccctgcg aggggaacgc cggcccagcc ggggcggttc ccgagctcgt
     1321 aatcgatttc gagcgcggct ttctcgatga gaaagagcga cagcagattg cgggcgattg
     1381 cggcatcgcg cggccagagc gggcaatcgg ccatggtcgc gacataggtg tcgaggaagc
     1441 gggtgctcac ctgatcgcgc cactcgataa ccgccgggct gagctcgttg cgatagtccg
     1501 gccgattggc tgtcgcgtga tcgagcgccg tccacgccgc gtagtcgaaa gaccgcatca
     1561 tcgaggcgac atcgcgcacg atcgaatgct ttgcccgccg gtccgcgaga gaaagctgcg
     1621 gcggcccttc gaaatcgacg atcgaaacgt cattttggac gacgagggtc tgaccgagat
     1681 ggtagtcgcc atggcaccgc gacagcagaa cgtcggtgtt gccggcagcg gcggagcgaa
     1741 tctgcgcgag gacggcgccg cgttgctgcg cgagctcgtc gagcgcggca atcgcgtcgg
     1801 ctgacagacg gccgcgcacc gcgtgcagcg cgtcgagcgc cgcttcgacg cgccgagccg
     1861 tctcctcgcc ccagtctctc atgcgctcgg gcccggcgcg ctcgacgacg aaggccggat
     1921 cgccctcctc gacatagagc acgcgatgaa actcggccgt ccggcgcgcc agctgctcga
     1981 tatagacgag ataggcggcg tggtgctcgg tcggcaaggg ttgcggctcc gcgggcgcga
     2041 cgcgaatgtc ctgcaagaac cgatcgagat aggcgagggt ataggtccag ccgtcgccct
     2101 ggttgcggat cagcgcgtgc accacgccca gcgcggtatc gttgccggcg gcgtcgacga
     2161 ggtgaatgct gccgaagagc gtcggcgtgt tggcataggc gtcggtgccg gtcaagaacg
     2221 cgcccagctc gatctcggga tgcgtgccga cggcgacccg gcgatagaac ttcatgat
//
LOCUS       DASUCJ010000136         1361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_551650, whole genome shotgun sequence.
ACCESSION   DASUCJ010000136 DASUCJ010000000
VERSION     DASUCJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551650"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFU90_01710"
     CDS             <1..226
                     /locus_tag="VFU90_01710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5478512.1"
                     /translation="AVFAHAMELLGVGPDESIMVGDRYDRDIEGAHAAGMRAIWIRCR
                     GEEVPHGARPPEAIIDSIGGLPGALAVFPA"
     gene            312..722
                     /locus_tag="VFU90_01715"
     CDS             312..722
                     /locus_tag="VFU90_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEU5478513.1"
                     /translation="MRKLLIAASSAAALFVIAAAHPAPAKADFKICNQSSEKISVAIA
                     YHDQDAGNWVSRGWWNLDPGTCKVPITGELRDRYYYLYGDGDQHYWGGEHSFCVDNSD
                     KFTLNESDTTCDYDYEGFLEVDTGDSTGYTYTFQ"
     gene            883..>1361
                     /locus_tag="VFU90_01720"
     CDS             883..>1361
                     /locus_tag="VFU90_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478514.1"
                     /translation="MSARPNGSPIDPHAAPAVPVPVRHGIETIVVKGAREHNLKNIDL
                     TLPRNQLIVITGLSGSGKSSLAFDTIYAEGQRRYVESLSSYARQFLGQMEKPDVDYIE
                     GLSPAISIDQKGASRNPRSTVGTVTEVYDYLRLLYARIGEPHCPNCGRKITPQTAEQ"
BASE COUNT          267 a          460 c          425 g          209 t
ORIGIN      
        1 ggcggtcttc gctcatgcga tggaactgct cggcgtcggg ccggacgaga gcatcatggt
       61 cggcgatcgt tacgatcgcg acatcgaagg cgcccacgcc gccggaatgc gcgcgatctg
      121 gatccgctgc cgcggcgaag aagtgccgca cggcgcgcgg ccgcccgagg cgatcatcga
      181 ctcgataggc ggtctgccgg gcgcgctcgc cgtctttccc gcataggcct gcgtgacgca
      241 gcgcacatag attcgccgtc gtgcacaacg ctgccgatga ccctgtcaga acgattggga
      301 ggtccatcgt catgcggaaa ctattgatcg ccgcgagcag cgcggcagca ctgttcgtca
      361 tcgcggcggc gcatccggcg cccgccaagg cggacttcaa gatttgcaac cagtcgagcg
      421 agaagatctc ggtggcgatc gcgtaccacg accaagacgc gggcaactgg gtatcgcgcg
      481 gctggtggaa cctcgacccg ggcacgtgca aggtcccgat cacgggagag ttgagagacc
      541 gctactacta tctgtacggc gacggcgacc agcactactg gggcggcgag cattccttct
      601 gcgtcgacaa cagcgataag ttcacgctca acgaatccga cacgacgtgc gactacgact
      661 acgaggggtt cctcgaggtc gacacgggcg actcgaccgg ctatacgtac acgttccagt
      721 aacggacggc cccgggaagc ggtcgagcct cagctcgacc ggagacgggc ctgcaaatgc
      781 aagaccgcgg cggtggaccg taagggtcga ccgctccacg ttttccctta cctgacaacc
      841 atgaagggcg accgctccgc ccttctcgaa ccggatacgt ccatgagtgc gcgtccgaac
      901 ggcagtccca tcgatcctca cgccgccccg gcagtgccgg tgccggtgcg ccacggcatc
      961 gagacgatcg tcgtcaaagg cgcgcgcgag cacaacctca agaacatcga cctgacgctt
     1021 ccgcgcaacc agctcatcgt catcaccggc ctctccggct cgggcaaaag ctcgctcgcg
     1081 ttcgacacga tctacgccga agggcagcgg cgctacgtcg agtcgctctc gtcgtacgcg
     1141 cgccagttcc tcgggcagat ggagaagccc gacgtcgact acatcgaggg cctctcgccc
     1201 gcaatctcga tcgaccagaa gggcgccagc cgcaacccgc gctcgaccgt cggcaccgtg
     1261 accgaagtct acgactacct ccgcttgctg tacgcgcgga tcggcgagcc gcactgtccg
     1321 aattgcggac gcaagatcac gccgcagacg gcggagcaga t
//
LOCUS       DASUCJ010000137         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_842032, whole genome shotgun sequence.
ACCESSION   DASUCJ010000137 DASUCJ010000000
VERSION     DASUCJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842032"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>1007
                     /locus_tag="VFU90_01725"
     CDS             168..>1007
                     /locus_tag="VFU90_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5478515.1"
                     /translation="MMGERVGHHPAGRRAFASCPGIRGAGAVALGLCALVVAGCASLV
                     PGSNFAKVPSHALGAPIPTSLGLQFDRAARRHDGKSAFRLLSVGVDGLLFRAQMIGVA
                     TRTIDLEYFIFRQDDTGQLLSDALLRAADRGVRIRLLVDDADRNDGDEQIAALSADPN
                     IQIRIYNPFAYRGRDELIRDLEFTFDASRLDYRMHNKLMVVDNAIALVGGRNIGDAYF
                     QVDPESQFGDDDVFAAGPIVRKLSDSFDEYWNSALAIPVAALDEARTSSARLAAYRVA
                     LEQH"
BASE COUNT          170 a          329 c          325 g          183 t
ORIGIN      
        1 ctggctgcac ggtgtcgtcg actcccgcaa cgaggcgcgc aaggtggtta ccatcgcgcg
       61 caataccgag ggcgtgacgt cggtcgtgag ccagcttgaa gtccggccgg atcactgacg
      121 gccaccacgc tgcatttccc cgcgaagcct gcgtcgaccc tggcggaatg atgggcgagc
      181 gcgttgggca tcatcccgcc gggcggcgcg cctttgcctc gtgtccaggc atccgcggcg
      241 cgggcgccgt cgcgcttgga ttgtgcgcac tggtcgtcgc cggatgtgca tcgctggttc
      301 ctgggagcaa cttcgcaaag gtgccctccc acgcgctcgg agcgccgata ccgacatccc
      361 tcggtcttca attcgaccgc gcggcgcgcc gacacgacgg gaaatccgcc tttcgactgc
      421 tctcggtcgg agtcgacggc cttttgtttc gcgcgcagat gatcggggtc gcgacgcgaa
      481 cgatcgatct cgaatacttc atcttccggc aggacgatac cggacagttg ttaagcgacg
      541 ccctgctgcg ggccgccgat cggggcgtcc gcatccggct cctggtcgac gacgcggaca
      601 gaaacgacgg agacgagcag atcgccgcgc tcagcgcgga tcccaacatc cagatccgga
      661 tctacaatcc gttcgcatac cggggtcgcg acgagttgat tcgcgatctt gagttcacct
      721 tcgacgcgtc gcgcctcgac tatcgaatgc acaacaagct gatggtggtc gacaacgcga
      781 ttgcgctcgt cggcggacgc aacatcggcg atgcgtactt tcaggtcgac ccggaatcgc
      841 aattcggcga tgacgatgtc ttcgcggcgg gcccgatcgt ccgaaagctc tcggattcat
      901 tcgacgagta ctggaacagc gcgctggcga tcccggttgc ggcgctggac gaggcacgca
      961 cgtcgtcggc gcgactggcc gcgtaccgcg tcgcgctcga gcagcat
//
LOCUS       DASUCJ010000138         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_933796, whole genome shotgun sequence.
ACCESSION   DASUCJ010000138 DASUCJ010000000
VERSION     DASUCJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933796"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFU90_01730"
     CDS             complement(<1..262)
                     /locus_tag="VFU90_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478516.1"
                     /translation="MSQLGLFHDGRRVLFDDATGTITYVPALIEPPVALAWFEQLAAG
                     VEWRSERRRVYDRDVDVPRLVSGYRLDDQRLPPVLAEAARRAS"
     gene            complement(282..>1278)
                     /locus_tag="VFU90_01735"
     CDS             complement(282..>1278)
                     /locus_tag="VFU90_01735"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5478517.1"
                     /translation="ALAMWMTWKCALVNSPFGGAKGGVICDPKEMSMQELENLTRRFT
                     SEISVIIGPEKDIPAPDVYTTPQIMAWIMDTFSMQHGYSIPGVVTGKPISIGGSLGRD
                     KATARGCLYVIDEAMAVLRKPIEGARVAIQGFGNAGMYAAQLMEQRGYRVVAVSDSAG
                     GVANPQGLDVRRLTAHKLETGSIVGFTGGERLGNREVLEYDCDVLVPAALEKVITAEN
                     APRIKAKIVAEAANGPTLPQADDILFERGIMVLPDILANAGGVTVSYFEWVQDLQANF
                     WEEDEINERLKRIVTRAFRETHEQSERHGVSMRQGAYCVAVDRVAEATKLRGLYP"
BASE COUNT          209 a          440 c          403 g          226 t
ORIGIN      
        1 cgctcgcccg gcgcgccgct tccgccagca ccggcggcag ccgctggtcg tcgaggcggt
       61 atcccgagac gagccgcggc acgtcgacgt cgcgatcgta cacccggcgc cgctcgctgc
      121 gccactcgac gccggccgca agctgctcga accaagccaa cgcgaccggc ggctcgatca
      181 gcgcagggac gtaggtaatc gttccggtgg cgtcgtcgaa gagaacgcgc cggccatcat
      241 ggaacaaacc cagctgcgac atccggcacg ccgatttccc gctacgggta gaggccgcgc
      301 agtttcgtcg cttcggcgac ccggtcgacg gccacgcagt acgcgccctg gcgcatcgag
      361 acgccgtgcc gttccgactg ctcgtgcgtc tcgcggaagg cgcgcgtcac gatgcgtttg
      421 agccgctcgt tgatctcgtc ctcttcccag aagttggctt gcagatcctg cacccactcg
      481 aagtacgaaa ccgtcacgcc gccggcgttg gccagaatgt cgggaagcac catgatgccg
      541 cgctcgaaga gaatgtcgtc ggcttgcggg agggtcggtc cgttggcggc ttcggcgacg
      601 atcttcgcct tgattcgcgg ggcgttttcc gcggtgatca ccttctccaa cgccgccggc
      661 acgagcacgt cgcaatcgta ctcgagcacc tcgcgattgc ccagccgttc gccgccggta
      721 aagccgacga tcgagccggt ttcgagcttg tgcgcggtca ggcgccggac gtcgagcccc
      781 tgcggatttg cgacgccgcc ggccgaatcg gagaccgcga cgacgcgata gccgcgctgc
      841 tccatgagct gcgcggcgta catgccggcg ttaccgaatc cctggatggc cacgcgcgca
      901 ccctcgatcg gcttgcgcaa gacggccatc gcttcgtcga tgacgtacaa acagccgcgc
      961 gcggttgctt tgtcgcgccc cagcgatccg ccgatcgaaa ttggtttgcc ggtgacgacg
     1021 ccgggaatcg aatagccgtg ctgcatcgag aacgtgtcca taatccacgc catgatctgc
     1081 ggcgtggtgt acacgtcggg cgccgggatg tctttttcgg gcccgatgat gaccgagatt
     1141 tcactggtga accggcgcgt caggttctcc agctcctgca tcgacatctc tttgggatcg
     1201 cagattacgc cgcccttggc gccgccgaag ggactgttga cgagcgcaca cttccacgtc
     1261 atccacatcg ccagcgcc
//
LOCUS       DASUCJ010000139         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1147583, whole genome shotgun sequence.
ACCESSION   DASUCJ010000139 DASUCJ010000000
VERSION     DASUCJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147583"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..805)
                     /locus_tag="VFU90_01740"
     CDS             complement(152..805)
                     /locus_tag="VFU90_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478518.1"
                     /translation="MFNKRVAFSTVLIGAVLVACTSHGGGSSFVPQGSTYTIPSFDSQ
                     LLITANLPPRTVGEELPSAGLGTIKSTYWKATLGGYTQMRYSQSLGFPTGTKITLRNL
                     SRKTPHTLNVVAKIKGPPAKFPKNPSLKTLPHGNGVLTVGYASGVIQPGKSVTITLSQ
                     PGIYLIGCAYHYSQGMHDVFVVGSKAKPGQQATPPPKSTSNPSSSPSTGPSPTGYGG"
     gene            complement(860..>1274)
                     /locus_tag="VFU90_01745"
     CDS             complement(860..>1274)
                     /locus_tag="VFU90_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5478519.1"
                     /translation="DVRSARTIADRYVALDPDSARALYSRGIVALKGGDAATARDDFG
                     KLLHVNPSYAVAHYNLALAEERLGRYDAAERELRTALALAPTYARAQLALGAVLLKEG
                     RRREARDAFAQATRDAAADPALQNIAAAMRDSIAI"
BASE COUNT          220 a          388 c          425 g          241 t
ORIGIN      
        1 cctcggccgc cgcatgccgc caacgctaag gcgaaggcca gcagtgcgat ccccccgatt
       61 ttttgcatag ttcaattctc ctaaagcaaa cgcttacagt gtgtgagacg ttaacagcga
      121 cgaatgaagg aagtattaat cgcggcggac gttaaccgcc gtaacccgtc gggctcggac
      181 ccgtgctcgg actcgacgac ggattcgacg tcgatttcgg cggcggcgtg gcttgctgtc
      241 cgggttttgc cttgctgccg acgacgaaaa cgtcgtgcat gccttggctg taatggtacg
      301 cgcacccgat gagatagatg ccgggctgcg aaagcgtaat cgtcaccgac ttcccgggtt
      361 ggatcacgcc gctcgcgtag ccgaccgtca gcacgccgtt gccgtgcggg agcgttttca
      421 gcgacgggtt cttgggaaac ttcgccggcg gtcccttgat tttggccacc acgttgagag
      481 tgtgcggcgt ctttcttgaa aggttgcgca gcgtgatctt cgtacccgtc gggaacccga
      541 gcgactgcga gtaacgcatt tgcgtgtagc cgcccagcgt cgctttccag tacgtcgact
      601 tgatcgtccc cagacctgcg ctcggcagct cctcgccaac cgtacgcggc ggaaggttgg
      661 ccgtaatgag cagttgagag tcgaaagagg ggatcgtata cgtgctgcct tgcggcacga
      721 acgacgatcc gcctccgtgg ctggtgcacg cgacgagcac ggcgccgatc agcaccgtcg
      781 agaatgccac gcgtttgttg aacatgggaa cctcctgcgg gtagaccggt taaatctaac
      841 gggcgaggat gaataattgt taaatcgcga tcgaatcgcg catcgcggcg gcgatatttt
      901 gcagcgcggg gtcggccgcg gcgtcgcggg tcgcctgggc gaaggcgtcc cgcgcctccc
      961 ggcggcgccc ttcctttaga aggacggcac ccagggcgag ctgcgcgcgc gcgtacgtcg
     1021 gcgccagcgc gagcgccgta cgaagctcgc gttcggcggc gtcgtagcgt cccagccgct
     1081 cctcggcgag cgccaggttg tagtgggcca ccgcatagga ggggttgacg tggagcagct
     1141 tcccgaagtc gtcgcgggcc gtcgccgcgt ctccgccctt caaggctacg atcccgcgcg
     1201 agtaaagggc ccgggcggag tcgggatcca gggcgacgta gcggtcggcg atcgtccgtg
     1261 ccgagcgcac gtcg
//
LOCUS       DASUCJ010000140         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1315740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000140 DASUCJ010000000
VERSION     DASUCJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1155)
                     /locus_tag="VFU90_01750"
     CDS             complement(112..1155)
                     /locus_tag="VFU90_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5478520.1"
                     /translation="MRHRSLTLSSVAVLALGAGALAVTAASRDSARHAVDVVDFGKTV
                     RATSPAFDARLAPASDAPIKQFRIPITDATLEIPNGVKYQGWTFGGTVPGPVLRVRVG
                     DQVRITVVNESPIPHSIDFHAARIPANVAYRMIMPKDSISFQFEARDAGAFMVHCGTP
                     PVTMHIMQGMYLPIIVDPKDGWGTKADKEFVIVQSEFYAKAGDTTASSAAMVPMQPDW
                     QAAMAKNASYVVFNGRAFQYKDHPLNVNVGDRVRLFVVNAGPSMNSDFHIVGAVFDRV
                     YPDGNPHHALTGVQTYTVPAGGGAVFETVFDKNASGEGLYPFVTHSFADAEKGAVGII
                     QVGVPKLYAHMDH"
BASE COUNT          200 a          394 c          390 g          212 t
ORIGIN      
        1 gaatgtcgac cactgtgcgc tgcgcggtgg ggttctcgag catcgacgcg atcatgtgtc
       61 cggtcctcgc aaaggagtgc gccgggctgt cccggcgcac gactcgactg ctcagtgatc
      121 catgtgggcg tacagcttcg gcacgccgac ctggatgatt ccaaccgctc ccttctcggc
      181 gtcggcgaag ctgtgcgtca cgaacgggta cagcccttcg ccgctcgcat tcttgtcgaa
      241 caccgtctcg aacaccgcgc cgccgccagc cggcaccgtg tacgtctgca cgcccgtgag
      301 cgcgtgatgg ggattgccgt cgggatacac gcggtcgaac acggcgccca cgatgtggaa
      361 atcgctgttc atgctcgggc ccgcgttcac cacgaacagt cgcacgcggt cgccgacgtt
      421 cacgttcagc ggatggtcct tgtactggaa cgcgcggccg ttgaacacga cgtagctcgc
      481 gttcttcgcc atcgccgcct gccagtcggg ctgcatcggc accatcgccg cgctgctcgc
      541 cgtggtgtcg ccggctttgg cgtagaactc gctctgcacg atcacgaatt ccttgtcggc
      601 cttggtgccc cacccgtcct tcggatcgac gatgatcggg agatacatcc cctgcatgat
      661 gtgcatcgtc accggcggcg tgccgcagtg caccatgaac gcaccggcgt cgcgtgcctc
      721 gaactggaac gagatggagt ccttcggcat gatcatccgg taggcgacgt tcgccgggat
      781 gcgcgcggcg tgaaaatcga tcgagtgcgg aatgggcgat tcgttcacga cggtgatgcg
      841 cacttgatcg ccaacgcgca cgcggagcac cggaccgggc accgtaccgc cgaaggtcca
      901 cccctgatac ttcacgccgt tcgggatctc gagcgtcgcg tccgttatgg gaatccgaaa
      961 ctgcttgatc ggcgcatcgg acgcgggcgc gagccgcgcg tcgaacgccg gcgacgtcgc
     1021 gcgaacggtc ttcccgaaat ccacgacgtc cacggcatgg cgcgcgctgt ctcgcgacgc
     1081 ggcggtaacg gcgagggcac cggcaccgag ggcgaggacg gccacgctcg agagggtgag
     1141 ggaacgatga cgcacgggac aatctccatt tagctgtgag gcgccggact tcggcg
//
LOCUS       DASUCJ010000141         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_933822, whole genome shotgun sequence.
ACCESSION   DASUCJ010000141 DASUCJ010000000
VERSION     DASUCJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933822"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFU90_01755"
     CDS             <1..356
                     /locus_tag="VFU90_01755"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEU5478521.1"
                     /translation="YYKGNATITVPQGYKVTIKFTNDDPSLAHSIGVDSAYTTFPATF
                     TNPQPIFSGAISSDPTDPTKATQPTKSGTVTFTADKAGSYTLNCFIPGHAMSGMWIHF
                     DVSSDGKAGVQTSAS"
     gene            complement(410..>1819)
                     /locus_tag="VFU90_01760"
     CDS             complement(410..>1819)
                     /locus_tag="VFU90_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5478522.1"
                     /translation="DSVAPSPVALPDSFSSATSDSALAADSTLDTMQVAAFASGTSVS
                     GEAPDSTFWSGLGDSTLTGLVHEALRASPDVRAARSRFFEARSQRRLASLDLVPTITA
                     TASVARQQLSAAQFPGSVGALPRQDLWDGGVDASWELDVFGRVSHNVRAGRAFEESAD
                     EQVRDAQLSLAAEVARTYFELRGAQSELAVAERNADNQRRTMKLTEQRLSAGSGTAFD
                     VERARAELSLTLAQTPTIEARIAASRNQLAVLLGRTPESLPANLLSPGPLPSLPNVVK
                     VGSPADLVKRRPDVRSAERQLAAQTMLIGAAQADYLPRISLAASAGYTAPTFNALGNT
                     GTPRFFVGPVLSWPFLDMGRVKTRVDIAQAQADQAKAQYTSAVLGAISETESAIVSYD
                     RSRARLSSLSEAVRASTHALQLAQLRYEAGETDFLQVLDAQRTLLSAESELAVGRTSA
                     VTALVALYKAVGGAWPAGR"
BASE COUNT          304 a          648 c          594 g          273 t
ORIGIN      
        1 gttattacaa ggggaacgcg acgatcaccg tgccgcaagg ctacaaggtg accatcaaat
       61 tcacgaacga tgatccgtcg ctggcgcaca gcatcggtgt cgacagcgcg tacacgacgt
      121 tcccggcaac gttcacgaat ccgcagccca tcttttctgg cgcgatcagc tccgatccta
      181 cggatccgac caaggcgacg cagccgacca agtccgggac ggtgacgttc accgccgaca
      241 aggccggaag ctacacgctg aactgcttca tcccgggcca tgcgatgtcc ggaatgtgga
      301 tccatttcga cgtgtcgtcc gacggtaagg caggcgttca gacgagcgcg agctgacgca
      361 gcgaagggcg gccggcggaa acgcccggcc gccctgtttc atcgccggct catctgccgg
      421 ccggccaggc cccgccgacc gccttgtaca acgcgacgag cgcggtgacg gcggacgtgc
      481 gcccaacggc cagctcgctc tcggcgctca gcaaggtgcg ttgggcatcg agcacctgca
      541 ggaaatccgt ttcccctgct tcgtagcgga gctgcgcgag ctggagcgcg tgtgtgctgg
      601 cgcgcaccgc ctcgctcagg ctggacaggc gtgcccgcga gcgatcatag ctgacgatgg
      661 cgctttccgt ttcactgatt gcgcccaaca ccgccgaggt gtattgggcc ttggcctgat
      721 cggcctgggc ctgggcgatg tcgacgcgcg ttttcacgcg gcccatgtcg aggaacggcc
      781 agctgagcac cggcccgacg aaaaatcgcg gcgtgccggt gttgcctaac gcattgaacg
      841 tcggcgccgt gtagccggcg ctcgcggcca gcgaaatccg cggcaggtag tcggcctgcg
      901 ccgcgccgat caacatcgtc tgcgccgcga gctgccgctc ggcgctccga acatcgggac
      961 gccgcttcac cagatcggcc ggcgatccca ccttgacgac gttaggcagc gagggcagcg
     1021 ggcccgggct cagcaggttg gcaggcagcg attccggcgt ccggcccaac aacaccgcca
     1081 gctggttgcg gctcgcggcg atccgcgcct cgatcgtcgg cgtctgcgcc agcgtcaggc
     1141 tcaactcggc gcgtgcgcgc tcgacgtcga acgcggtgcc actccccgcc gacaagcgct
     1201 gttccgtcaa cttcatggtg cgacgctggt tgtcggcatt ccgctccgcg accgcaagct
     1261 cgctctgcgc cccgcgcagc tcgaagtacg tccgcgcgac ttcggcggcc aaactgagtt
     1321 gggcatcgcg gacctgctcg tccgcggact cctcgaacgc ccggcccgcg cgcacgttgt
     1381 ggctcacgcg cccgaagacg tccagctccc aggacgcatc cacgccgcca tcccagagat
     1441 cctgccgcgg cagcgcgccc acgctgccgg gaaactgcgc cgccgagagc tgttgccggg
     1501 cgacgctggc cgtggccgtg atcgtcggga cgagatcgag ggacgccagc cgccgttgcg
     1561 atcgcgcctc gaagaaccgc gagcgcgccg cgcgcacgtc gggactcgcg cgcagcgcct
     1621 cgtgcaccaa ccccgtgagc gtgctgtcgc ctaacccgga ccagaacgtg ctgtcgggcg
     1681 cctcgccgct cacgctcgtg cccgacgcga acgccgccac ctgcatcgtg tcgagcgtcg
     1741 aatcggccgc gagcgccgaa tccgacgttg cagacgaaaa agaatcgggc agcgccaccg
     1801 gcgagggcgc gacagaatc
//
LOCUS       DASUCJ010000142         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1239493, whole genome shotgun sequence.
ACCESSION   DASUCJ010000142 DASUCJ010000000
VERSION     DASUCJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239493"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFU90_01765"
     CDS             complement(<1..1352)
                     /locus_tag="VFU90_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5478523.1"
                     /translation="MKPTDIRVPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRHDDAY
                     LINWKSNVFPGILGRTCDRPCEPACRRGRVEENNGTKPEPVAICRLKRVAADNKDAAA
                     VRARMPKPAAKKNGRRIACVGGGPASLTVARDLAPLGYHVTVFEADAKGGGFMRTQVP
                     RFRLPEEVIDEEVGYILDLGVELRGNTRIDSMKDLLAQKFDAVFVGSGAPRGRDLEIP
                     GRREAAANIHIGLDWLASVYFEHIKKIGRRVIVLGGGNTAMDCCRSSRRLGGEDVKVI
                     VRSGFEEMKASPWEKEDAQHEGIPILNYLVPKTFVHEAGKLTGMTFEKVRAEHDAKGR
                     RKLVPTGEPDALFECDDVLIAIGQENAFPWIERDIGIEFDAEWDMPKVDKITMQSSNP
                     KVFFGGDAAFGPKNIIWAVAHGHDAAVSIDKMLSGEDIRERPEPGVSLISQKMGIHEW
                     "
     gene            1621..>1922
                     /locus_tag="VFU90_01770"
     CDS             1621..>1922
                     /locus_tag="VFU90_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478524.1"
                     /translation="MLYRLHEFNRNLFNPLLAWSQTSAKMLSSPDSWLSQLPFAQRAA
                     ANCELVYRLGKEYEKPAFGIHTVNAHGQDVAVLERIALAKPFCNLLRFKRFSDDVPT"
BASE COUNT          324 a          645 c          586 g          367 t
ORIGIN      
        1 ctccactcgt ggatgcccat cttctgcgag atcaggctga cgccgggttc cgggcgttcg
       61 cggatatcct cgccgctgag catcttgtcg atggacaccg ccgcgtcgtg gccgtgtgcc
      121 accgcccaga tgatgttctt cggtccgaac gccgcgtcgc cgccgaagaa cactttcgga
      181 ttcgacgact gcatggtgat cttgtcgact ttcggcatgt cccattccgc atcgaattcg
      241 atgccgatgt cgcgctcgat ccacgggaac gcgttttcct ggccgatcgc gatcagcacg
      301 tcgtcgcatt cgaacagtgc gtccggctcg ccggtcggca ccagtttgcg ccgccccttg
      361 gcgtcgtgct cggcgcggac tttctcgaac gtcatgccgg tgagcttgcc ggcttcgtgc
      421 acgaaggttt tcggcacgag gtaattgagg atgggaatgc cctcgtgctg ggcgtcttcc
      481 ttctcccacg gcgaggcctt catctcctcg aatccagaac gcacgatcac cttcacgtcc
      541 tcgccgccga ggcgccgcga cgagcggcag caatccatcg cggtgtttcc gccgccgagc
      601 acgatcacgc gccgaccgat cttcttgatg tgctcgaagt agaccgaagc gagccaatcc
      661 agaccgatgt ggatgttcgc ggcggcctcg cggcggcctg gaatctccag atcgcgtccg
      721 cgcggtgcgc cggagccgac gaacaccgcg tcgaatttct gcgcgagcag gtccttcatc
      781 gaatcgatgc gcgtattgcc gcgcaattcg acgccgaggt cgaggatata accgacctcc
      841 tcgtcgatca cttcctcggg caggcggaag cgcggaacct gtgtacgcat gaagccgccg
      901 cccttggcgt cggcctcgaa aacggtgacg tggtagccca gcggcgcgag gtcgcgcgcg
      961 accgtgagcg acgccgggcc gccgccgacg caggcgatgc gcctgccgtt cttcttcgcc
     1021 gccggtttcg gcatgcgcgc acgcaccgcc gcagcatcct tgttgtcggc ggcgacgcgc
     1081 ttgaggcggc agatcgctac cggttccggt ttcgtaccgt tgttttcctc gacgcgcccg
     1141 cgccggcacg ccggttcgca ggggcggtcg caggtgcggc cgaggattcc cggaaagacg
     1201 ttcgatttcc agttgatcag gtaggcgtcg tcatggcgac cggcggcgat caggcggatg
     1261 tactcgggaa cgggggtgtg cgcgggacag gcccactggc aatcgaccac tttatggaaa
     1321 tagtcgggaa cgcgaatatc cgtgggcttc accggtgcct ccgcgaaaac gttgtcgatc
     1381 cgagtctact cgcgcgctcg gcgcctgcct agaacccgtt tttatccgtt cgtgttgagc
     1441 gtagcgaagc gaagtcgaaa cacaatgtaa atggctcggc gacccttcga tttcacgcct
     1501 tcggcgctac gctcagggcg aacgggatcg tgtggaaatg tccggacgct tgatgcggcg
     1561 cggcaagaaa tccgccacaa tgccccgccg ccgggccagg gcccgccatc cggaacatcg
     1621 atgctctatc gcctgcacga attcaaccgc aacctgttca acccgctgct ggcctggtcg
     1681 cagaccagcg cgaagatgct ctcctccccc gacagctggc tctcgcaact gcccttcgcg
     1741 caacgcgccg cggccaactg cgaactcgtc taccggctcg gcaaggaata cgaaaagccc
     1801 gctttcggca tccacaccgt caacgcgcac ggccaggacg tcgccgtcct cgaacgcatc
     1861 gcgctggcaa aacccttttg caacctgctg cgcttcaagc gcttcagcga cgacgtgccg
     1921 ac
//
LOCUS       DASUCJ010000143         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1438162, whole genome shotgun sequence.
ACCESSION   DASUCJ010000143 DASUCJ010000000
VERSION     DASUCJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438162"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VFU90_01775"
     CDS             complement(<1..1212)
                     /locus_tag="VFU90_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5478525.1"
                     /translation="MDVQARDLSGGPSPLARIAPEVWWAAGLVAVLALGLALRLHGIH
                     NPILDHPGWRQGDTAAIARNFARLQFDVMHPQTIYNGPPPNYVELELQIVPFVAAALY
                     KAFGIHEIFGRLTTLAFSLAAIVTVAYFARWLFAGAAAGLIAAFFFAVFPGSVYYGRT
                     FTPDTAMVFFLTAALYAAARLTLDDPSLAPRPLARTSALLALAYLAKPVAVVALVPLG
                     ALVWQRLRAGLPTRPTAIAVMTLVPLLILWLYDRRIASYAEWHWASGITTLHVLPGLR
                     DAFTSFAAFGAKLGAFRTALGMLRQTMLGTVGFLGSVAAFIALPWVAARSKPLLWGWL
                     AATLVYIFVVVTVERVDYYMYPMLPLCALVAGGAGARLIAFVRETAAGPAVRYAIFAA
                     LGIFGVAGALQG"
     gene            complement(1188..1526)
                     /locus_tag="VFU90_01780"
     CDS             complement(1188..1526)
                     /locus_tag="VFU90_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5478526.1"
                     /translation="MNLAAFTLLQRAVPNHDRPLQYSAIYSLAFLAGGVSNYYLNRVW
                     TFRSTGHAVREGMQFLSVSVIALAVGLGVSALVSPWLGRGHKTWFVATVAGIFINFFL
                     NKYWTFKHVT"
     gene            complement(1606..>1945)
                     /locus_tag="VFU90_01785"
     CDS             complement(1606..>1945)
                     /locus_tag="VFU90_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478527.1"
                     /translation="DAFSKIVHAVNGQTVANESLEVAFTALMIAVLIGGWRRLRPSYV
                     AYLALSILVPMCTSSLMSMPRFALVLFPMFAILARWGERPWVNNLILAFSLPLLGLFT
                     VLFADWYWVA"
BASE COUNT          413 a          620 c          668 g          244 t
ORIGIN      
        1 cccttgcagc gcgccggcta cgccgaatat tccgagcgcg gcaaagatcg cataacgcac
       61 ggccgggccg gccgcggttt cgcgaacgaa cgcgatgaga cgcgcgcccg cgccgcccgc
      121 gaccagcgcg cacagcggca gcatcgggta catatagtag tcgacgcgct cgacggtgac
      181 gaccacgaag atgtagacga gcgtcgccgc gagccagccc cacaacagcg gcttgctgcg
      241 cgccgcgacc cacggaagtg cgatgaaggc ggctaccgac ccgagaaacc cgacggtgcc
      301 cagcatcgtt tggcgcagca tcccgagcgc cgtccggaac gctcccagtt tggcgccgaa
      361 agcggcgaag ctcgtgaacg catcgcgcaa tcccggcagc acgtgcagcg tcgtgattcc
      421 gctggcccag tgccattcgg catacgacgc aatccgccgg tcgtagagcc acagaatcag
      481 caacggcacg agcgtcatga cggcgatggc ggtcgggcgc gtcggaagtc cggcacgcag
      541 acgctgccag acgagcgcgc cgagcgggac gagcgcgacg accgcgaccg gcttggccag
      601 gtaggccagc gcgagcaggg cgctcgtgcg cgcaagcggc cgtggcgcga gcgacggatc
      661 gtcgagcgtg agccgcgccg cggcatagag ggcggcggtg aggaagaata ccatggccgt
      721 gtccggcgta aacgtgcgcc cgtagtagac gctgccggga aacacggcga aaaagaatgc
      781 cgcaatcagc ccggcggcgg cgccggcaaa cagccagcgc gcgaagtagg cgaccgtcac
      841 gatcgccgcc aggctgaacg ccaaggtcgt cagccggccg aagatctcgt gaatgccgaa
      901 ggccttgtac agcgcggccg ccacgaacgg aacgatttgc agctccagct ctacgtagtt
      961 cgggggcggc ccgttgtaga tcgtttgcgg atgcatgacg tcgaactgga gacgcgcgaa
     1021 gttgcgcgcg atcgcggcgg tgtcgccctg gcgccatccc gggtgatcga gaatcggatt
     1081 gtggatcccg tgcaggcgca gtgcgagtcc gagcgctagg acggcgacga ggccggcggc
     1141 ccaccacact tccggggcga tacgcgcgag cggcgacggg ccgccgctca ggtcacgtgc
     1201 ttgaacgtcc aatacttgtt caggaagaaa ttgatgaaga tgccggcgac ggttgccacg
     1261 aaccaggtct tgtgcccgcg gccgagccac gggctcacca gtgccgacac gcccaagccg
     1321 accgccagcg cgatcaccga cacgctgagg aactgcatgc cttcgcgcac ggcgtgcccg
     1381 gtagatcgaa acgtccagac gcggttcaga tagtagttcg acacgccgcc cgcgagaaac
     1441 gccagcgaat aaatcgcgga gtactgcagc gggcggtcgt ggttgggaac ggcgcgctgc
     1501 agcagcgtaa aggcggccag attcacgagg aagccggagg cgcctacgat accgaattta
     1561 acgaattgcc gaacgccgcg gcgtgcggcg agcggagcaa aaccgctacg caacccagta
     1621 ccagtcggcg aacagaacgg tgaacaggcc gagcagcggc agtgagaacg ctaggatgag
     1681 attgttcacc cacggacgct cgccccatcg ggcgaggatc gcaaacatcg gaaagagcac
     1741 gagtgcgaag cggggcatcg acatgaggct ggaggtgcac atcggcacga ggatcgacag
     1801 cgccaggtag gcgacgtacg acggccgcag ccgtcgccag ccgccgatca gcaccgcaat
     1861 catcaacgcc gtgaacgcga cctcgaggct ctcgttggcg acggtctgac cgtttacggc
     1921 gtggacgatc ttcgaaaacg catcg
//
LOCUS       DASUCJ010000144         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_475296, whole genome shotgun sequence.
ACCESSION   DASUCJ010000144 DASUCJ010000000
VERSION     DASUCJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475296"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFU90_01790"
     CDS             complement(<1..1802)
                     /locus_tag="VFU90_01790"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU5478528.1"
                     /translation="MTWKSIAAAAVAAFLLGAVHRPAPLIVYSAPAASRPAGADSIHP
                     TTATLPDGRIAAPVGESTFVGTNPLGMALTPDGRFAIVSNDDERTNDLPAPRTDSPPV
                     SGFSLAVVDTRTMQLASTYRDPAAAFFIGVAAVAEAGGTIVLASDGPHGVVRVFDLGA
                     DGRLTPEARTIALPSNGGRRAFPAGIAVAGGGKTAYVADNFNGAVIAIDVASRAVTRS
                     FAAGNFPLDVAAAGRHVLISAGGLAAYSPLDPPARFPQFAAPAFDPNRSSALDVLNLL
                     ADGTAAADPDVVRMDPAPDGSKVVGGAAPGAIALRRDGRLAYVAMSNVDRVAVVSLAS
                     ETPRVVRGLDLRLYPDAPYGAEPSAEALSKDGKRLYVALAGLNAVAVLDARSSTRYRY
                     GLIPTGWYPTALALSPNGRYLYVASAKGVDGWGMLSRVDLKHTSLVKATMSALRYDRT
                     AGIAKENPIVPPLRSGKRSSAIARIVYVAVGRTAYDGMLGDLKDAAGAALGNGNPDFE
                     RYPPSVTPNVHALARTFALADNFYASDADIEIARQYATQAGAPAYAQLTARLRSNRNP
                     FDGSDDPEDYERAGYLFNALARAGASFRDYGGLL"
     gene            1891..>2299
                     /locus_tag="VFU90_01795"
     CDS             1891..>2299
                     /locus_tag="VFU90_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478529.1"
                     /translation="MDLLPRILSVATAVPPYLLDQNDVTRRVSIALGPRSPEIVRLLP
                     MFGNTGIVRRYSCVPIEWYEEPHDWPERNQIYLDSAIELLESAARSALARAGRLPEEV
                     DAIVAVSTTGIATPSLDALLMERMGLRRTVPRLP"
BASE COUNT          388 a          756 c          791 g          364 t
ORIGIN      
        1 tgcagcaagc cgccgtaatc gcgaaacgac gctccggccc gggccagcgc gttgaacagg
       61 tagccggcac gctcgtaatc ttcgggatcg tcgctgccgt cgaacggatt gcggttcgat
      121 cgcaggcgcg ccgtcagctg cgcgtacgcc ggcgctccgg cttgcgtagc gtactgccgc
      181 gctatttcga tatcggcatc cgaggcgtag aagttatccg ccagggcgaa cgtgcgggcc
      241 agcgcgtgca cgttcggcgt tacgctcggc ggatatcgtt cgaagtccgg gttgccgtta
      301 ccgagggccg cgccggcggc atctttgaga tcgccgagca tcccgtcgta cgcggtccgg
      361 ccgacggcga cgtacacgat ccgcgcgatt gccgagctgc gcttcccgga acgcagcggc
      421 gggacgatcg ggttctcctt ggcaataccg gccgtgcggt cgtagcgaag cgcgctcatc
      481 gtcgctttta ccagcgacgt gtgtttgaga tcgacccgcg aaagcattcc ccatccgtcg
      541 acgcctttgg cacttgcgac gtagagatag cggccgttgg gtgagagcgc gagcgccgtg
      601 ggataccatc cggtgggaat cagcccgtaa cggtagcgcg tcgacgagcg ggcgtcgagc
      661 accgcaacgg cgttcaagcc ggccagcgcg acgtacaagc gtttcccgtc tttactgagc
      721 gcttcggcgc tcggttcggc accgtacgga gcgtccgggt agagacgcaa atccaaaccg
      781 cggacgacgc gcggcgtttc cgaggcgagc gaaacgaccg cgacgcggtc gacgttcgac
      841 atcgcaacgt aggccagccg gccgtcgcgg cgcaacgcaa tcgcacccgg cgccgcgccg
      901 cccacgactt tgctgccgtc cggcgccgga tccattctca cgacgtcggg atcggcagcc
      961 gcggttccgt cggcgagcag gttcaagacg tcgagtgccg acgaacgatt cggatcgaac
     1021 gcaggcgcgg cgaattgcgg aaaccgtgcg ggcggatcga gcggcgagta cgcggccaga
     1081 ccgcccgcgc tgatcagcac gtgccggccc gcggcggcga cgtcgagcgg gaagtttccg
     1141 gcggcaaaac tccgcgtaac cgcacgcgaa gcgacgtcga tagcgatcac ggcgccgttg
     1201 aagttatccg caacgtaggc ggtttttccg ccgcccgcta cggcgatgcc ggcgggaaag
     1261 gcgcgtcgtc cgccgttcga cggcaacgcg atcgtgcgtg cttccggcgt cagccgtccg
     1321 tcggcgccga ggtcgaagac gcggacgacg ccgtgcggac cgtcggaggc gagcacgatc
     1381 gtgcctcccg cctcggcaac ggctgcaacg ccgatgaaga acgcagcagc cggatctcga
     1441 tacgtgctcg cgagctgcat cgtgcgcgtg tcgacgaccg cgagcgaaaa tccggaaacc
     1501 ggcggcgaat ccgtgcgcgg tgcgggcaaa tcgttcgtgc gttcgtcgtc gttgctgacg
     1561 atcgcgaagc ggccgtccgg cgtcagcgcc attccgagcg gattggtccc cacgaacgtg
     1621 ctctcgccca ccggcgccgc gattcggccg tccggaagag ttgccgtggt ggggtggatg
     1681 gagtcggcgc cggccgggcg gctcgcggcc ggcgcggaat agacgatgag tggtgcgggg
     1741 cgatggacgg cgcccagcaa aaaggcggcc accgccgccg ctgcgatcga tttccaagtc
     1801 acgggacgtt aggcttacga acgccggccg cacggaccct gctcgcccga caggaaagac
     1861 gctgcgggcg cggcacggaa cccccgcaag gtggaccttc ttccccgcat actctccgtc
     1921 gctacggccg tgccgccgta tctgctcgat caaaacgacg taacgcgtcg cgtcagcatc
     1981 gcgctcggac cgcgttcgcc cgagatcgtt cggctgttgc cgatgttcgg aaacacgggt
     2041 atcgtacgcc gttattcgtg cgtcccgatc gagtggtacg aggagccgca cgattggccc
     2101 gagcgcaacc aaatctatct cgattccgcg atcgaactgc tcgaatcggc cgcgcggtcc
     2161 gcgctcgctc gggcaggacg actgcccgag gaagtggacg caatcgtcgc cgtttcgacg
     2221 accggtatcg cgacgccgag tctcgacgcg ctgctgatgg aacggatggg gctgcgcagg
     2281 acggtacccc gtctgccga
//
LOCUS       DASUCJ010000145         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1208935, whole genome shotgun sequence.
ACCESSION   DASUCJ010000145 DASUCJ010000000
VERSION     DASUCJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208935"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFU90_01800"
     CDS             <1..700
                     /locus_tag="VFU90_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5478530.1"
                     /translation="SQWPAVARGSDGPIEAPGIPQNDAGADLLLEIAHAFSKRNRLTV
                     LVTGAATELASAIQREPSIVNRISVVAMGFDDWSSGDEFNVQNDPAAWRAVLAADVPL
                     VIGSAAVTKRDLRLTRDDAAQLIGVHGQIGAYLNRLFDGWLDAHPELVSKVVTSNAWV
                     IWDDVVTAYLLGMTHGERRQRPVLRADLRFGHPATARRLTWLTSIDAQRVWSDLARKI
                     DAAGSTFSRARHSE"
     gene            complement(729..>1689)
                     /locus_tag="VFU90_01805"
     CDS             complement(729..>1689)
                     /locus_tag="VFU90_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478531.1"
                     /translation="GHPWSTSAYVTDFVEKTTPDDYRLLRPEHDDAGGADMPAAGYVW
                     DAAVRKGISLRDYGEYGEPDPDASHGPDSVRTRSAIASLAPYTSPTYPSFDLAIPDQR
                     RADAWLAEFREFERTGAMPALEILHLPNDHTAGARRGLHTPKALMADNDLALGRIIEA
                     ISRSRFWRSTVIFVLEDDAQAGPDHVDSHRSVLLVASAWSRHGVSHRFVNTTDVLATI
                     EEILGLDPLSAFDRFGRPLREIWRSEPDLSPYAALTPSQPLSDLNVAEGPDARRSARF
                     DLSRPDEIDDDTFNRVLWRAMKGPRAPYPVQRRAAVQEFVRAR"
BASE COUNT          278 a          567 c          572 g          272 t
ORIGIN      
        1 ctcgcaatgg cccgcggtgg cgcgtggaag tgatggaccg atcgaagcgc ccggaatccc
       61 gcagaacgac gccggcgccg atctgctgct cgagatcgcc cacgccttct cgaagcgaaa
      121 ccgcctcact gtcctggtca cgggtgccgc gaccgagctc gcgtccgcaa tccagcgcga
      181 accctcgatc gtgaatcgca tttcggtggt ggcgatggga ttcgacgact ggagctccgg
      241 cgacgagttc aacgtgcaga acgatcccgc ggcatggcgc gccgtgctgg ccgccgatgt
      301 gccacttgtg atcgggagcg cagcggtcac caagcgcgat ctgcgactga cccgcgacga
      361 cgccgcacag ctcatcggcg tgcacggcca gatcggcgcg tatctgaacc gcctgttcga
      421 cggctggctc gacgcccatc ccgagttggt ctcgaaggta gtgacgtcga acgcgtgggt
      481 gatctgggat gacgtcgtca ccgcgtatct gctcggcatg acgcacggcg agcggcgcca
      541 acgtccagtg ttgagggcgg atctgcggtt cggtcaccca gcgacggcgc gccggttgac
      601 gtggctcacc agcatcgatg cccagcgcgt gtggagcgac ctggccagaa agatcgacgc
      661 agctggttcg actttttcgc gcgcgcgtca ttccgagtag aggccgcgtg ccgccgacac
      721 gccggagctc atcgcgctcg gacaaattcc tgaacggcgg cgcgcctttg aaccggatat
      781 ggcgcgcgcg gccccttcat cgcgcgccac agcacgcggt tgaatgtgtc gtcatcgatc
      841 tcgtccgggc gcgagaggtc gaatcgtgcc gagcgccgcg catcgggccc ctccgccaca
      901 ttgaggtcgg agagtggctg cgagggcgtc agcgcagcat agggcgagag atcgggctcc
      961 gagcgccaga tctcacgcaa cgggcggcca aatcggtcga acgccgacag tgggtcgagc
     1021 ccgagaatct cctcgatggt cgccagcacg tccgtggtgt tgacgaatcg gtgcgacaca
     1081 ccgtgtcggc tccacgccga tgcgaccagg agcaccgacc ggtgcgagtc cacatggtcg
     1141 gggccggcct gggcgtcgtc ctcgagcacg aagatcaccg tgctcctcca gaagcgtgac
     1201 ctggagatcg cttcgatgat gcggcccagc gcgagatcgt tgtccgccat cagcgccttc
     1261 ggcgtatgca gcccgcgacg cgcgccggcg gtgtgatcgt taggcagatg gaggatctcg
     1321 agtgcgggca tcgcgccggt ccgttcgaac tcgcgaaact cggcgagcca cgcgtcggcg
     1381 cgccgctggt ccgggatcgc gaggtcgaac gacggatagg tgggcgacgt gtatggcgcc
     1441 agcgacgcga tagccgaccg cgttcgcacc gagtcgggcc cgtgcgacgc gtccggatcc
     1501 ggctcgccgt actccccgta atcgcgcagg ctgatcccct tcctcaccgc ggcgtcccac
     1561 acgtaccccg ccgcgggcat gtccgcgccg ccggcatcat cgtgctccgg tcggagcaac
     1621 cggtaatcgt ccggcgtcgt cttctccacg aagtcggtga cgtacgccga ggtcgaccac
     1681 ggatgcccc
//
LOCUS       DASUCJ010000146         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_735182, whole genome shotgun sequence.
ACCESSION   DASUCJ010000146 DASUCJ010000000
VERSION     DASUCJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735182"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFU90_01810"
     CDS             <1..209
                     /locus_tag="VFU90_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5478532.1"
                     /translation="GRLYAIEDVCTHDGGPLDQGTLEGACVVCPRHGATFDVRTGEAL
                     TLPAVVPLMTFALDVEGDDVYVDA"
     gene            227..2374
                     /locus_tag="VFU90_01815"
     CDS             227..2374
                     /locus_tag="VFU90_01815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5478533.1"
                     /translation="MRTLFRVAAALVLMAAAVGALFAINVALGMRAHARYAGTISGLR
                     MLHAPVSILRDDRGVPHIVARDQHDLFFAQGYAEGSDRLFQMDLLRRFVEGDLAEVLG
                     RAALSSDERERAVPVRAIVQAQWRSLDAGSKETLGAFGEGVNAAIDREPLPVEFRILA
                     YRPAPWTPEDSLAVSMATVLDLIDDWNDVEPRDSVYRRGGLRALRARYPLSDPCYDAP
                     LTAGLGGIGPGRACGLRAALLRELDGARVPVGSNEWAAGANRSLTGRALLANDPHLAL
                     RMPGVWYLADLRAPGFHAAGATFPGAPGIVLGHDERVAWGATDGTVASLSVFEPPARL
                     DSQGWQTERFRVRFGGTVTRRYYRDATTFGVTTKGGKFVLVRWGAYLHPISPATTFLN
                     LDRAATIEDAEKALAAFPGPTHNFAIADTSGRAAYVLAGPVPDDPVWARWFHPAGDLA
                     KRYGTIPFEKLPKVAASRDAVVWTSNNKMYASGYPWRLSPQFAAPYRAYRVAQLLRAR
                     AKYDVAYFAAMQMDVLSIPERELAHELAPALRAVDPGSADALAHWDGEMDGDSSAATL
                     AEELRLQVTHREKDAMPATLEIARRSPAALALTPLPPADRWASAGAVTPLHAFASLGI
                     AFLDGSSLPGDGDAFTLHVQYTGYSQSFRAVWDVGNWDAGGITLPQGESGEPGSGHYT
                     DEAGAWIAGRLWPLPFSDAAVGRTAVDRETLAP"
     gene            2442..2756
                     /gene="rplU"
                     /locus_tag="VFU90_01820"
     CDS             2442..2756
                     /gene="rplU"
                     /locus_tag="VFU90_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5478534.1"
                     /translation="MYAIIETGGKQYKVAEGDVIRCDLLDSQVGSDVTFDRVVLTGNG
                     SDVQVGTPLVSGAKVSGTVLRQAKDKKILVFRYKPKKRVRKLNGHRQRFAEVKITKIA
                     PA"
     gene            2773..3135
                     /locus_tag="VFU90_01825"
     CDS             2773..3135
                     /locus_tag="VFU90_01825"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU5478535.1"
                     /translation="MVEVVFHRDSRGRLSSFFAGGHAEFADQGEDVVCAAVSAILQAA
                     RLGLEEYAGVALEAHQQHGSLSVAWPPECRDDERVRAIAATAELSIRRIAEQFPEHVG
                     CTSHDESAARRNTPGEAG"
BASE COUNT          509 a         1063 c         1132 g          514 t
ORIGIN      
        1 acggtcgcct ctatgcgatc gaagacgtct gcacgcacga cggcggcccg ctcgatcaag
       61 ggacgctcga gggcgcgtgc gtggtatgcc cgcgccacgg cgcgacgttc gacgtccgca
      121 ccggcgaggc gctcacgctg cccgccgtcg ttccgttgat gacgttcgcg ctggacgtcg
      181 aaggcgacga cgtgtacgtg gacgcgtagg agccgcgagg aagttcgtgc ggacgctctt
      241 tcgcgttgcg gcggcgctgg tgctgatggc ggcggcggtg ggcgcgctct tcgccataaa
      301 cgtcgcgctg ggaatgcgcg cgcacgcgcg ttacgcgggg acgatctcgg ggctgcgcat
      361 gctgcacgcg ccggtatcga tcttgcgcga cgatcgcggg gtgcctcata tcgtcgcgcg
      421 cgaccagcac gatctgttct tcgcgcaagg ttacgccgaa ggctcagacc ggctctttca
      481 aatggatctg ctccggcgat tcgtcgaagg cgatctggcc gaagtgctgg gacgcgcggc
      541 gctgagcagc gacgaacgcg aacgcgcggt tccggtgcgc gcgatcgtgc aagcgcaatg
      601 gcgctccctc gacgccggct ccaaagagac gctcggggcg ttcggcgaag gcgtcaacgc
      661 ggcgatcgac cgcgaaccgc tgccggtcga gtttcggatt ttggcgtacc gtccggcgcc
      721 gtggacgccc gaggattcgc tggccgtttc gatggcgacg gtgctcgact tgatcgacga
      781 ctggaacgac gtcgagccgc gcgactccgt ctatcgccgc ggcggactgc gagcgcttcg
      841 ggcacgctat ccgctgagcg atccgtgtta cgacgcaccg ctgaccgccg ggctcggcgg
      901 catcgggccg gggcgggcgt gcgggctgcg cgccgcgctc ttgcgcgaac tcgacggcgc
      961 acgcgtaccg gtcggaagca acgaatgggc ggccggagcg aaccgttcgc tcaccggacg
     1021 cgcgctgctc gccaacgatc cgcacttggc cttgcggatg ccgggcgtgt ggtacttggc
     1081 tgatcttcga gcaccgggct ttcacgcggc gggcgcgacg tttccgggtg cgccgggcat
     1141 cgtgctcggt cacgacgagc gggtcgcgtg gggggcgacc gacggaaccg tcgcgtcgct
     1201 ctcggtcttc gaaccgccgg cgcggctcga ttcgcaaggc tggcaaaccg aacgtttccg
     1261 cgtgcggttc ggcgggacgg tgacgcggcg ttactatcgc gacgcgacta cgttcggtgt
     1321 gacgaccaaa ggcggtaagt tcgtgctggt gcgctggggc gcgtacctgc atcctatctc
     1381 tcccgccacc acgttcttga atctcgaccg cgcggccacg atcgaggacg ccgaaaaagc
     1441 gctggccgct tttccggggc cgacccacaa cttcgcgatc gccgacacga gcggacgcgc
     1501 cgcctacgtg ctggcggggc cggttcccga cgatcccgtc tgggcgcgct ggtttcatcc
     1561 ggccggcgac ctcgcgaaac gatacggcac gattccgttc gaaaaactgc ccaaggtcgc
     1621 ggcgtcgcgc gacgcggtcg tctggacctc caacaacaag atgtacgcaa gcggttatcc
     1681 gtggcgcttg agcccgcagt tcgcggcgcc gtatcgcgcc taccgggtag cgcagctgct
     1741 gcgcgcgcgc gcgaaatacg acgttgcgta ctttgcggcg atgcagatgg acgtgctctc
     1801 gatccccgag cgcgagctcg cgcacgagct ggcgcccgca ctgcgcgcgg tcgatccggg
     1861 atcggcggat gcgctggcgc actgggacgg cgagatggac ggcgactcga gcgcggcaac
     1921 gctcgccgaa gaacttcgct tgcaggtcac gcaccgcgaa aaagacgcga tgccggcgac
     1981 gctcgagatc gcccggcgtt ccccggccgc gctggcattg acgccgctgc cgcccgcgga
     2041 ccgctgggct agcgccggcg cggtcacgcc gctgcacgcg ttcgcgtcgc tcggaatcgc
     2101 gttcttggac gggagctcgc tgccgggcga cggggacgcg ttcacgctgc acgtccagta
     2161 caccggatat tcgcagagtt tccgggccgt ctgggacgtc ggcaactggg atgccggcgg
     2221 gataacgctc ccgcaaggcg agtcgggtga gcccggctcc gggcactaca ccgacgaagc
     2281 cggcgcgtgg atcgccggcc gtctctggcc gctgccgttc tccgacgccg ccgtcgggcg
     2341 taccgcggtc gaccgcgaga cgctcgcccc gtaggcggag ccggcggcga aacggttttc
     2401 cgtttgacac ggccgagcgg ggctggtatc gtaccgaagt catgtacgcc atcatcgaaa
     2461 ccggcggcaa gcagtataag gtcgccgagg gcgacgtcat ccgctgcgac ctgctcgaca
     2521 gtcaggtcgg gtcggacgtt acgttcgacc gagtcgtgtt gaccggcaac ggcagcgacg
     2581 tccaggtggg aacgccgctg gtcagcggcg ccaaagtcag cgggacggtg ctgcgccaag
     2641 cgaaggacaa gaagattctg gtctttcgct acaaacccaa aaagcgcgtt cgcaagctga
     2701 acggccaccg tcagcgcttc gccgaagtca agatcacgaa gatcgccccg gcgtaggcac
     2761 tcggcgccgg ccatggtcga agtcgttttc catcgggaca gccgcggccg gctgtcctcg
     2821 ttttttgccg gcggccacgc cgagttcgcc gaccaaggcg aggacgtcgt ctgcgccgca
     2881 gtatcggcga ttctgcaagc cgcgcggctc ggtctcgagg aatacgccgg cgtcgcgctc
     2941 gaggcgcacc agcagcacgg cagcttatcg gttgcgtggc cgccggaatg ccgcgatgac
     3001 gaacgcgtcc gggcgattgc ggcgaccgcc gaactctcga ttcgccggat cgccgaacag
     3061 tttccggagc acgtcggctg cacgtcgcac gacgaatcgg cggcgaggcg caacactccg
     3121 ggcgaagccg ggtaagtagg gcaacagcac cgaaggaggc ccctatgact gacagtttcc
     3181 gatcgacacc gccgcagcag cagtggtccg ccccgaat
//
LOCUS       DASUCJ010000147         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1346519, whole genome shotgun sequence.
ACCESSION   DASUCJ010000147 DASUCJ010000000
VERSION     DASUCJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346519"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFU90_01830"
     CDS             <1..326
                     /locus_tag="VFU90_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5478536.1"
                     /translation="SGALDRFEQAWHRGAAGKARSAEVRLTFESLPLLLRNLTPARWK
                     LLEAVKRSGPTSINELARLLGPNYKNVHTDVTRLVELGLIERMTDQRIVVAWDTIVAE
                     MKLAA"
     gene            complement(371..1570)
                     /locus_tag="VFU90_01835"
     CDS             complement(371..1570)
                     /locus_tag="VFU90_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5478537.1"
                     /translation="MARRIACFTLCCATLLARASPDLPFIPIPEIDTAPDSGATLGLI
                     PVVLSNNDAGEIDRILAPDVIHSQYFGWGATWRTFRSPSEDRKWSVVAGAKERVESEF
                     DAEYDAGLLRASDWSWIVHSFYERDGTGRFYGLGNRSTLGNETTFIDRRYGFEWTAAR
                     NFTHELQLAWSVHGLDAEVGQRALDFLPAIETVYPEQPGVGHVRELQQRLVLAWDTRD
                     SDVVPRRGDRWAAFAGFAPDRFGGGNGYSFLGVDLTSLRPIGPAVTLVGHTALRFMPS
                     YAKAPFWELSNLGGDRSVTGGAQPLRGFGDGRFVDRNSFEASVEARIDVRSFHMFGTN
                     LELEVAPFVDSGKVFAAMSGDPFTHLHVAGGVGFRLVASPFVVGYLDVGFGGEKAAVF
                     SGIDYPF"
     gene            complement(1605..3209)
                     /locus_tag="VFU90_01840"
     CDS             complement(1605..3209)
                     /locus_tag="VFU90_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478538.1"
                     /translation="MSALWRPNPGTEAFWSYEHFEADEVGALLHPLHGPGAGTIVAQF
                     GDAIPLFPGLLPVIFPSSFHQTDADFSAHDRTHLDATQLTLTQDVGESTRAKLILGYR
                     HLYNDTAIDVDATSLPFANTALQNSSSQKSAELQLSGTTPDRKLDWIGGLYWFRDDGS
                     APSEIPAQSPAYQAFFAATGGPVVPYPVIESNALENLSTAGYLHGEYHLTDRWAAAAG
                     VRRTEDRRKLSENSYAATPLGQSCTIIDATTGLPANNGGPCPPIDKSVRFSYWSWELS
                     TRYQLTDHVNAYFRSGRAQRSGGWNVPLSTLQDQPFRPEQLTDYELGLKSDLLGGRLA
                     INADVFYGNYDDMQRLLPQLVGGTPTTYVINAGKARVSGAEVEADMDLAGGWSMRAAF
                     GWTDARYRQFEYTPSPGLPPVNLAGNEFAQTPRFNAALGVGYVALLPGGSLRLHADYA
                     WQDTVQFNVVNDFNKQGAYGTLNARVTYAVPGDPWELALFGTNLTDRRYAVTGGTVSP
                     NAMAWQIPGPPREVGVEFTYRFGRAG"
     gene            complement(3219..3932)
                     /locus_tag="VFU90_01845"
     CDS             complement(3219..3932)
                     /locus_tag="VFU90_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5478539.1"
                     /translation="MALPHEVGRRRASGVVAGALALVVLVPAVAPALADDTGATVDLG
                     TITVTARRRDEKIIDAPVAVTVETGEQLQDQNAVLFDDVGREVPNVRMTQSPTSVSAL
                     DVTIRGQSAIRSAIAYDPAVGIYVDGVYVANGQAAMNTLLDIDKVQIIRGTQGTLFGR
                     DNTGGSILFTTRRPELKRWSSEVAVDGGSDSLFTGRAIANVPLGDTAALRFAYQDNER
                     DGFGSSLASGQDDFGNQHR"
     gene            complement(4042..>4683)
                     /locus_tag="VFU90_01850"
     CDS             complement(4042..>4683)
                     /locus_tag="VFU90_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5478540.1"
                     /translation="RARSLHSGIDVEFLRDDIDSVLDESREGLVRVKRIVQDLRQVSP
                     NAPDDAWESIDLHRALDRAVDAVRGDIRYKATIEREYGEVPAIECLPARLSQVFVNLL
                     VNAGQSIESKGTITIATGTADAEAWVRIEDTGCGIRPGNLSRIFEPFFTTRPAGQGAG
                     LGLSIASSIVRKHGGRIDVASDVGRGSCFTVWLPLRCAEASAAPEALVPALIE"
BASE COUNT          803 a         1553 c         1577 g          750 t
ORIGIN      
        1 tctcaggagc gctggaccga ttcgagcagg cgtggcatcg cggcgcggcc gggaaagcgc
       61 gaagtgccga ggtgcggctg accttcgaga gccttccgct gctcctcagg aatctcacgc
      121 ccgcgcgttg gaaattgctc gaagcagtca agcgctcggg accgacgtcg atcaacgagc
      181 tggcgcggct actgggaccc aactacaaga acgtccacac cgatgtgacg aggctggtcg
      241 aactgggatt gatcgagcgg atgaccgatc agcggatcgt ggtcgcgtgg gacacgatcg
      301 tcgccgaaat gaagctggct gcatgaggtg atgcactggg ccgctcgatg catttcgact
      361 ggtctccgat tcagaacgga tagtcgatcc cgctgaacac cgcggccttc tcgccgccga
      421 atcccacgtc gagatagccg acgacgaagg ggctcgcgac gaggcgaaac cccacaccgc
      481 cggcgacgtg caggtgcgtg aacgggtcgc cggacatcgc cgcgaagacc tttcccgagt
      541 cgacgaacgg cgcgacctcg agctcgaggt tcgtgccgaa catgtgaaag ctgcgtacat
      601 cgatgcgcgc ctccacgctc gcctcgaagc tgttgcgatc gacgaagcga ccatcgccga
      661 acccgcgcag cggctgcgca ccgccggtga cgctgcgatc cccgcccaga ttggaaagct
      721 cccagaacgg cgccttcgcg tacgacggca tgaagcgcaa tgcggtatgc ccgacgagcg
      781 tcacagcggg accgatcggc cgcaggctcg tgaggtcgac gccgagaaag gagtagccgt
      841 tgccgccgcc aaacctgtcc ggcgcgaagc ccgcgaacgc cgcccagcgg tcaccgcgcc
      901 gtggtacgac gtcggagtcg cgcgtgtccc aggcgagaac gagacgctgc tgcagctcgc
      961 gcacgtgacc gacgcccggt tgctctggat agacggtctc gatcgccggc aagaaatcga
     1021 gcgcgcgctg cccgacttcc gcatcgagcc cgtgcacgct ccaggcgagc tgcagctcgt
     1081 gagtgaagtt gcgcgcggcg gtccattcga agccataccg gcgatcgatg aatgtcgtct
     1141 cgttgcccag cgtggagcga ttgccgagcc cgtagaagcg gccggttccg tcacgctcgt
     1201 agaacgagtg cacgatccac gaccagtcgc tcgcgcgcag cagtccggcg tcgtattcgg
     1261 cgtcgaactc gctttcgacg cgctccttcg cgccagcgac gaccgaccac ttgcggtctt
     1321 ccgatggaga gcggaacgta cgccaggtgg caccccagcc gaagtactgg ctgtgaatga
     1381 cgtctggcgc gaggatgcga tcgatttcgc ccgcgtcgtt gttcgacagg acgaccggaa
     1441 tcaagccgag cgtcgcgccg ctgtccggcg ccgtatcgat ctccgggatc ggaatgaacg
     1501 gcaagtcggg cgacgcgcgc gccagcagcg tcgcgcaaca gagggtgaag caggcgatcc
     1561 gacgggccaa ccagcgagcg ctgcggcggc gcacgacccg cggctcagcc ggcgcggccg
     1621 aatcggtacg tgaattcgac tccgacctcg cgcggcggcc ccggaatctg ccacgccatc
     1681 gcgttcggcg acacggtgcc gccggtgact gcgtaccggc gatcggtgag attcgtgccg
     1741 aacaaagcga gctcccacgg gtcaccgggc accgcgtagg tgacgcgcgc gttgagggtg
     1801 ccgtaggcgc cctgcttgtt gaaatcgttg accacgttga actgcacggt gtcctgccac
     1861 gcataatcgg cgtgcaggcg cagcgaaccg cccggcagca gcgcaacgta tccgacgccg
     1921 agcgcggcat tgaagcgcgg cgtctgggca aattcgttgc cggcgaggtt caccggcggc
     1981 agacccgggc tcggcgtgta ctcgaactgg cggtagcgcg cgtcggtcca gccgaacgcc
     2041 gcgcgcatcg accagccacc cgcgaggtcc atgtcggcct cgacctcggc accactgacg
     2101 cgcgccttgc cggcattgat cacataggtg gtcggtgtgc ctccgacgag ctgcggcagc
     2161 aggcgctgca tgtcgtcgta gttgccgtag aagacgtcag cgttgatcgc gagcctcccg
     2221 ccgaggaggt ctgatttcag cccgagctcg taatcggtca actgctcggg ccgaaacggc
     2281 tggtcctgca gcgtactcaa tgggacgttc cagccgcccg agcgctgcgc gcggcccgat
     2341 cgaaagtacg cgttgacgtg atcggtcagt tgatagcgcg tcgagagctc ccacgaccag
     2401 tacgagaaac gcacgctctt gtcgatcggc gggcacgggc cgccgttgtt cgcgggcagg
     2461 ccggtcgtcg catcgatgat cgtgcacgat tgcccaagcg gagtcgcggc gtacgagttc
     2521 tcgctgagct tgcgcctgtc ctcggtgcgc cgaacgcccg ccgcggccgc ccagcgatcg
     2581 gtcaggtgat attcgccgtg cagataaccg gcagtcgaaa ggttctcgag tgcattgctc
     2641 tcgatcaccg gatagggaac tacgggcccg ccggtcgccg cgaagaatgc ctggtacgcc
     2701 ggcgactgtg cggggatctc cgacggcgca ctgccgtcgt cgcggaacca gtagagtccg
     2761 ccgatccagt cgagcttccg gtcgggcgtg gtaccgctca actgcagctc ggcgctcttc
     2821 tggctggacg agttctgcag tgccgtgttg gcgaacggca gcgacgtcgc atcgacgtcg
     2881 atcgcggtgt cgttgtagag gtgacgatag ccgaggatca gcttcgcacg cgtgctctcg
     2941 ccgacgtctt gcgtcagggt gagctgcgtt gcgtcgagat gcgtgcggtc gtgcgcggaa
     3001 aaatcggcgt cggtctggtg gaagctggac gggaagatga ccggcaaaag acccggaaac
     3061 agcgggatcg catcgccgaa ctgagcgacg atggtgccag cgcctggacc gtgcagcggg
     3121 tgcagcagcg cgccaacctc gtcggcctcg aagtgctcat agctccagaa tgcctcggtg
     3181 ccggggttcg gacgccacag cgcgctcact ctcccctgct aacggtgctg gttgccgaaa
     3241 tcgtcctggc cgctcgcgag gctcgatccg aacccgtcgc gctcgttgtc ctgataggcg
     3301 aagcgcagcg ccgccgtatc gccgagcggg acgttggcga tggcgcgtcc ggtgaacagg
     3361 ctgtcgctgc cgccgtcgac ggcgacctcc gagctccagc gcttgagctc ggggcgccgc
     3421 gtcgtgaaca ggatcgagcc gccggtgttg tcgcgcccga acagcgtccc ctgcgtgccg
     3481 cgaatgatct gtaccttgtc gatgtcgagc agcgtattca tcgcggcctg cccgttggcg
     3541 acgtagacgc cgtcgacgta gatgcccacc gccgggtcgt aggcgatcgc cgagcggatc
     3601 gcgctctgcc cgcggatcgt cacgtcgagc gccgagaccg aggtcggcga ctgcgtcatg
     3661 cggacattcg gcacctcgcg tccgacatcg tcgaagagca ccgcgttctg atcctgaagc
     3721 tgctcaccgg tttccacggt gaccgcgacc ggcgcatcga tgatcttctc gtcgcgacgc
     3781 cgggcggtga cggtgatcgt cccgaggtcg accgtcgctc cggtgtcgtc cgccaatgcc
     3841 ggagcgaccg cgggcaccag gacgacgagt gcaagcgcac cggccacgac gccactcgca
     3901 cgtcgccggc ccacttcgtg gggaagtgcc atgcgcgatg ctaaccccgc cgtccggcgg
     3961 accgcaaccc gcggcagcgc gcctgcaatc cgccgcagcg cgcctgcgac gcgcgctggc
     4021 gcacctgcaa ccggtggcgg cctattcgat caaggccggc accagcgctt caggcgcggc
     4081 ggaggcttct gcgcaccgca acgggagcca cacggtgaag cagctgccac gcccgacgtc
     4141 gcttgcgaca tcaatgcgcc cgccgtgctt gcgcacgatc gagctcgcga tcgagagacc
     4201 gaggccggcg ccctgcccgg ccggccgcgt ggtgaagaac ggctcgaaga tgcgcgacag
     4261 attgcccgga cggatgccac acccggtgtc ctcgatgcgt acccacgctt cggcgtcggc
     4321 ggttccggtc gcgatggtga tcgttccctt gctctcgatc gactgtccgg cattgacgag
     4381 caggttgacg aacacctgcg acaaacgcgc cggcaggcac tcgatcgcgg gcacctcgcc
     4441 gtattcgcgc tcgatcgtcg ccttgtatcg aatgtcgccg cggaccgcgt cgaccgctcg
     4501 atcgagcgcg cgatgcaggt cgattgactc ccacgcgtcg tcaggcgcat tgggcgagac
     4561 ctggcggagg tcctgaacga tgcgcttgac gcgcaccagt ccttcgcggg attcgtcgag
     4621 cacggaatcg atgtcgtcgc gcaggaactc gacatcgata cccgagtgca gcgaccgcgc
     4681 gcg
//
LOCUS       DASUCJ010000148         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1483942, whole genome shotgun sequence.
ACCESSION   DASUCJ010000148 DASUCJ010000000
VERSION     DASUCJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483942"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="VFU90_01855"
     CDS             complement(<1..1621)
                     /locus_tag="VFU90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478541.1"
                     /translation="MQRLLSCALACALFVTPANAAAAANPASVDASYFSGLHWRMIGP
                     FRGGRALAVTGVPGQPERFYFGSVDGGVWGSYNAGRTWEPIFDAEPVGSIGAIAVAPS
                     DPDTLYVGSGEADMRSDIAYGNGMYKSVDGGKTWSHVGLDDTRQIGAIAVDPHDANVV
                     YVAALGHQYGPNAQRGVFKSVDGGKSWTKVLYRDADTGAIDVALDPAAPNVVYAALWQ
                     TRRPPWNVYPPSNGPGSGLYKSTDAGATWTRLSTGLPAHVGRIGIAPSPAKPGRVYAL
                     VDAGADDGGVYRSDDGGATWTHTDGEKRIWQRGWYFGGITADPKNAGTAYVMNTSTYR
                     STDGGKTFDAIKGAPGGDDYHTLWIEPGDPNRMILGSDQGAVVTVDGARTWSSWYNQP
                     TGQFYHVAVDAHYPYRVYGAQQDSGAAVEPSRSRYATINNWNFQPIDAGGESGSIAPD
                     LRHPDTAYGGSVTEERIDTGWERNLDPTLNYPSTLWRNTWTLPIVFSPADPAALYTSH
                     QRIFRSRDGGKTWTIVSPDLSRASEGKPSNLD"
     gene            complement(1651..2343)
                     /locus_tag="VFU90_01860"
     CDS             complement(1651..2343)
                     /locus_tag="VFU90_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478542.1"
                     /translation="MVRIGGHLRRGRAIALPVALLTLTAATVASPASDAGASAPTAAE
                     VLSRAQSRMNGLKTYQVPLTMSGSVKISFVSVPFHMSGTEYYSAPNRHALHMDSVPSL
                     ARGFENTMASMGGPKTWLATYDIAVRGIAPHGSHNAYVLFGTPRKPANVKNVTLWISA
                     KTYAVESVAFNYNNGATLNVDLGHRGYSPYQLPTHATVSAKFPQYAGQAQVQYGTYQI
                     NAPVPDSVFQKQ"
     gene            complement(2337..>2949)
                     /locus_tag="VFU90_01865"
     CDS             complement(2337..>2949)
                     /locus_tag="VFU90_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478543.1"
                     /translation="VLAVYRQHMMTRSGAIIPQAGAPAAAPRAVAGGVHISMFWWIII
                     IVVGFLILRSIMRSMSAPRYGPPGMPGAPPPGATPPGGMPPAGYGPGYGPGYGYGGGG
                     GSFWTGLLGGLGGAWLGNELFGQRGGGGAIGPADAAQPGAPDGGGGWGGDTSGWQSDA
                     GQADMGAASSGDWSGGGFGGGDSGGGGDFGGGDAGGGDSGGGW"
BASE COUNT          448 a          996 c          985 g          520 t
ORIGIN      
        1 catcgaggtt ggagggcttt ccttcgctgg cgcggcttaa gtcggggctg acgatcgtcc
       61 acgtcttacc gccgtcgcgc gaacgaaaga ttcgctggtg gctggtatag agcgcggccg
      121 gatcggccgg agaaaagacg atcggcagcg tccaggtatt gcgccaaagc gtacttggat
      181 agttcaacgt cggatcgaga ttgcgttccc atccggtgtc gatgcgctct tcggtgacgg
      241 atccgccgta cgccgtatcc ggatgccgga ggtcgggtgc gatgctcccg ctttcgccgc
      301 cggcgtcgat tggctgaaag ttccagttgt tgatcgtggc gtaccggctg cgactgggct
      361 cgacggcggc gccggagtcc tgctgcgctc cgtacacgcg gtacggatag tgcgcgtcga
      421 ccgcgacgtg atagaattga ccggtcggct gattgtacca ggagctccac gttcgcgctc
      481 cgtcgaccgt gacgaccgcg ccttgatcgc tgcctagaat catccggttc ggatcgccgg
      541 gctcgatcca aagcgtgtga tagtcgtcgc cgcccggcgc ccctttgatc gcgtcgaacg
      601 tcttgccgcc gtcggtcgaa cggtacgtgg atgtgttcat gacgtaggcg gtgccggcgt
      661 tcttgggatc ggccgtaatg ccgccgaaat accagccgcg ctgccaaatc cgcttctcgc
      721 cgtcggtgtg ggtccacgtc gcgccgccgt cgtcggagcg gtaaacgccg ccgtcgtccg
      781 cgccggcgtc gaccagcgcg tagacgcgcc ccggcttggc gggtgacggc gcgatgccga
      841 tgcgtccgac gtgcgcgggc aagccggtcg aaagacgcgt ccacgtcgcg cccgcgtcgg
      901 tcgatttgta gagaccgctg cccgggccgt tggacggcgg atagacgttc caaggcggac
      961 gccgcgtctg ccacagcgcc gcgtaaacga cgttcggtgc ggcagggtcg agcgcgacgt
     1021 cgatagcgcc ggtgtcggcg tcgcgataga gcaccttggt ccagctcttt ccgccgtcga
     1081 cgcttttgaa gacgccgcgc tgcgcgttgg gtccgtattg atgcccgagc gccgcgacgt
     1141 agacgacgtt ggcgtcgtgc ggatcgactg cgatcgcccc gatctgccgc gtgtcatcga
     1201 gcccgacgtg cgaccacgtt ttcccgccgt cgaccgattt gtacataccg ttgccgtacg
     1261 cgatgtcgga gcgcatgtcg gcttcgccgc tcccgacgta cagcgtatcc gggtcgctgg
     1321 gagcgactgc gatcgcgccg atcgagccga ccggttcggc atcgaagatc ggctcccacg
     1381 tgcgcccggc gttgtaactc ccccagacgc cgccgtcgac cgaaccgaag tagaagcgct
     1441 ccggctgtcc gggaacgccg gtcacggcga gcgctcggcc gccgcgaaac gggccgatca
     1501 tccgccagtg caggccgcta aaataggaag cgtcgacgga tgccggattc gcagcggcgg
     1561 ccgcgttggc gggcgtgacg aataacgcgc aggcgagcgc gcagctcagc agacgttgca
     1621 tgcatgctgc ttactccagt atgcgcgaat ctactgcttt tggaagaccg agtcggggac
     1681 cggggcgttg atttggtagg tgccgtactg cacttgcgct tgaccggcgt actgcggaaa
     1741 tttcgccgat acggtcgcgt gggtgggaag ttgatacggc gaataaccgc gatggccgag
     1801 atcgacgttc agagtcgcgc cgttgttgta attgaaggcg accgactcga cggcgtaggt
     1861 cttggcgctg atccacagcg tgacgttctt gacgttggca ggcttgcgcg gcgtgccgaa
     1921 gagcacgtac gcgttatggc tcccgtgcgg cgcgatcccg cggactgcga tgtcgtacgt
     1981 cgcgagccag gtcttgggtc cgcccatcga cgccatcgta ttttcgaaac cgcgcgcgag
     2041 gctcggcacc gagtccatat gcagcgcgtg ccggttcggg gcgctgtagt attcggtgcc
     2101 gctcatgtga aacggcaccg aaacgaaact gatcttgacg ctgccgctca tcgtgagcgg
     2161 cacttggtag gttttcaagc cgttcatccg gctctgcgcc cgcgacagca cctccgccgc
     2221 cgtcggcgcg gacgcccccg cgtcgctcgc gggtgaggcg acggttgcgg ccgtcagcgt
     2281 cagcagcgcg accgggagcg cgatcgctcg gccgcgccgc aggtgcccgc cgattcttac
     2341 catccgccgc cggaatcgcc gccgcccgcg tcacctccgc cgaagtcgcc gccgccccca
     2401 gaatctccgc cgccgaagcc gcctccgctc cagtcgccgc tgctggccgc gcccatatcg
     2461 gcctggcctg cgtcgctttg ccaaccgctc gtgtcaccgc cccagccgcc gccgccgtcg
     2521 ggggcgccgg gctgcgccgc gtcggctgga ccgattgcgc cgccgcctcc acgctggccg
     2581 aacagttcgt tcccgagcca ggcgccgccg agaccgccga gcagtccggt ccagaaactg
     2641 ccgcctccgc ctccgtagcc gtagcccggc ccgtaccccg gtccgtagcc tgccggcggc
     2701 ataccgcccg gcggggtcgc acccggcggc ggcgcacccg gcatcccggg cggaccgtaa
     2761 cgcggcgccg acatcgagcg catgatcgag cgcaagatca ggaagccgac cacgatgatg
     2821 atgatccacc agaacatcga aatatgcact cctcccgcga ccgctcgcgg cgccgccgcc
     2881 ggcgccccgg cttgcggaat aatggctccg cttctcgtca tcatgtgctg gcgatagacc
     2941 gccagtacg
//
LOCUS       DASUCJ010000149         1435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_184834, whole genome shotgun sequence.
ACCESSION   DASUCJ010000149 DASUCJ010000000
VERSION     DASUCJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184834"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFU90_01870"
     CDS             <1..598
                     /locus_tag="VFU90_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478544.1"
                     /translation="EALAALAANLWTVVWGPIGVVTVIFALLERVQRRDAGYGWSPRR
                     LPQVRDANVIPRSESIATAALGTLFVLWWASGAPLLPAGSPGTVEQTMMRAFVPILLV
                     ALASVTLAVANALHPYWTRLRLGLRAGVDGLTAFIAFGILVMSWNSVVAQAARLGHPA
                     LLAPGEWANLSLALGLWIAGVVSLVTCVTYAVRCARST"
     gene            648..1064
                     /locus_tag="VFU90_01875"
     CDS             648..1064
                     /locus_tag="VFU90_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478545.1"
                     /translation="MNRRLVLVALALLTALALPASVRAVSVQGNVRNVPYVIGFHPLN
                     SMSHPYLGQMHLNFSHGIISGTYTDMSVKPGSPFANAYNIPVSGGLSGSHVTLVIRQV
                     TFKGTMSGTKMSGSATIRGAIYEFAAQQGTPGSGKP"
     gene            1075..>1435
                     /locus_tag="VFU90_01880"
     CDS             1075..>1435
                     /locus_tag="VFU90_01880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5478546.1"
                     /translation="MTDLNPQAKQMADESMVRNLAAQANAIWPQEAPLFDRYALPAAP
                     RILDAGCGTGEIASRLAERFPGGSVLGVDIVDAHLELARARYARLAPRLQFEHQSIFE
                     LSAANDTFDLTVCRHVLH"
BASE COUNT          237 a          455 c          488 g          255 t
ORIGIN      
        1 cgaagcgctg gccgcgctgg cggcaaacct gtggacggtc gtttggggac cgatcggcgt
       61 cgtgacggtg attttcgcac tactcgaacg cgtgcagcgg cgcgacgccg gttacggatg
      121 gagcccgcgc cggctgccgc aggtgcgcga cgcgaacgtc atcccgcggt cggaatcgat
      181 cgcgacggcc gcgctcggta cgctgttcgt tctctggtgg gcgagcggcg cgccgctgct
      241 gcccgccggt tcgccgggaa cggtggagca gacgatgatg cgcgcgttcg tgccgatcct
      301 gctggtcgcg ctggcgagcg tcacgctcgc cgtcgccaac gcgctgcacc cgtattggac
      361 gcggctgcga ctgggtttgc gtgcgggcgt cgacggactc accgctttca tcgcgtttgg
      421 gattctcgtc atgagctgga atagcgtcgt cgcgcaagcg gcacggctcg gccatccggc
      481 actgctggca ccgggcgagt gggccaatct cagcctggcg ctcgggttgt ggatcgcggg
      541 cgtcgtctcg ctggtaacct gcgtcacgta cgcggtgcgg tgcgcgcgct cgacctgagt
      601 ggtaggatgg ggctgtaatc cgcaagcgaa ctaaaggaga ggtattcatg aatcgccggc
      661 tagttctcgt cgctctggcg ctgctgacgg cgctcgcgct tcccgcaagc gtccgggccg
      721 tctcggttca aggcaacgtt cgcaacgttc cgtacgtgat cggcttccat ccgctgaatt
      781 cgatgtcgca tccgtatctc ggacagatgc acctgaactt cagtcacgga atcatcagcg
      841 ggacgtacac cgacatgtcg gtaaaaccgg gcagcccgtt tgcgaacgcg tacaacattc
      901 ccgtatcggg cggcttgagc ggctcgcacg tcacgctcgt catccggcag gtcacgttca
      961 aaggaacgat gagcggcacg aagatgagcg gaagcgccac gatccgcggc gcgatctacg
     1021 agttcgccgc acaacagggg acgccgggaa gcggcaaacc gtaacctcgc cgcgatgacg
     1081 gacctcaatc cgcaagcaaa acagatggcg gatgagtcga tggtgcgcaa cttggccgcc
     1141 caggcgaacg ccatctggcc gcaggaagcg ccgctgttcg accgctacgc gctgccggcc
     1201 gcgccgcgaa ttctggatgc gggctgcggc acgggcgaga tcgcgtcacg cctcgccgag
     1261 cgctttcccg gaggcagcgt cttgggcgtc gatatcgtgg acgcgcacct ggaactggcg
     1321 cgcgcgcggt acgctcgcct ggcgccgcgg ctgcagttcg agcatcagag catcttcgaa
     1381 ctatcggcgg caaacgacac cttcgactta actgtttgcc ggcacgtgct gcacg
//
LOCUS       DASUCJ010000150         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1438259, whole genome shotgun sequence.
ACCESSION   DASUCJ010000150 DASUCJ010000000
VERSION     DASUCJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438259"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFU90_01885"
     CDS             complement(<1..238)
                     /locus_tag="VFU90_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478547.1"
                     /translation="MTAELSGWTAVALVPLTAVAGWMLRRLGRGALARRMRPHFVLGY
                     AALAAALVHMTWSMGSMGGANTRGIWLAIFALLAL"
     gene            complement(235..627)
                     /locus_tag="VFU90_01890"
     CDS             complement(235..627)
                     /locus_tag="VFU90_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478548.1"
                     /translation="MKRSIAFLALAAAVLWYALPATAQMKAPIPLTAAQFSASAPGAQ
                     VTLAVRVVSRERDLVRATVLERRDDAHYRATGTSVDLYLADQTPIVMGSAADVRAGTV
                     LFVDAIATTPGRADVKRATVVTSYVTVE"
     gene            753..>1163
                     /locus_tag="VFU90_01895"
     CDS             753..>1163
                     /locus_tag="VFU90_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5478549.1"
                     /translation="MWLTTLALRNPIAVTLFFVLVAVAGLAAFVRMGRSVLPPIAFPV
                     VTISAPYAGAAPDEIERAVVEPIEDQLAAIPNLDRIASFAQPGLARMEVRFRFGSNLQ
                     TDRSNVQQAVDAARANAPADLLAPNVTEQDPSQAP"
BASE COUNT          197 a          400 c          385 g          181 t
ORIGIN      
        1 ctagcgccag cagcgcgaat atcgcgagcc aaatcccgcg tgtattcgcg ccgcccatcg
       61 atcccatcga ccacgtcatg tggaccagcg cggcggccag cgccgcatag ccgagcacga
      121 agtgcggacg catccggcgc gctaacgcgc cgcgccccag ccgtcgaagc atccaaccgg
      181 cgacggcggt tagcggcacc agcgccaccg cggtccaccc gctgagctcc gcggtcactc
      241 gacggtgacg tacgacgtga cgacggtggc gcgtttgacg tcggcgcgac ccggcgtcgt
      301 ggcgatcgca tcgacgaata gcacggtgcc cgcgcgtacg tcggcggcgg atcccatcac
      361 gatcggcgtc tgatccgcga gatagagatc gaccgaggtg ccggtcgcgc ggtaatgtgc
      421 gtcgtcgcgg cgttcgagaa cggtcgcacg gacgaggtcg cgttcgcgcg agacgacgcg
      481 caccgccagc gtcacttgcg cgccgggcgc gctggccgaa aactgcgcgg ccgtcaacgg
      541 tatcggagct ttcatttggg cggtggccgg cagcgcgtac caaagcacgg cggcggcgag
      601 cgcgaggaat gcgatcgaac gtttcatgtc cggagcatac gccgccgcgc cgcgggcgac
      661 tacccgcctc gaccgccaaa ttacttccgc gcgaagtaat ttagccgaac cggcggcagc
      721 acagcgggcc gcggcggcta aggaaaacgc tcatgtggct aaccaccctc gcgctgcgca
      781 acccgattgc cgtgacgttg ttcttcgtgc tggttgccgt cgcgggtttg gcggcgttcg
      841 tccgtatggg gcgcagcgtt ctcccaccga tcgcctttcc ggtcgtcacg atttcagcgc
      901 cgtatgcggg tgcggctccg gatgagatcg agcgtgccgt cgtcgaaccg atcgaagatc
      961 agctggcggc gattccgaat ctggaccgga tcgcctcgtt cgcgcagccg gggcttgccc
     1021 ggatggaagt acgcttccgt ttcggcagca atcttcagac cgaccgcagc aacgtgcagc
     1081 aagccgtcga cgccgcccgc gctaacgcgc cggccgatct gctcgcgccc aacgtcaccg
     1141 agcaagatcc ttcacaggcg ccg
//
LOCUS       DASUCJ010000151         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_383486, whole genome shotgun sequence.
ACCESSION   DASUCJ010000151 DASUCJ010000000
VERSION     DASUCJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383486"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1089)
                     /locus_tag="VFU90_01900"
     CDS             complement(19..1089)
                     /locus_tag="VFU90_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478550.1"
                     /translation="MRAPYRVAAATLVAAVACGHPAPNANVAPAAPVANEPRLADIRQ
                     LTHGGANAEAYFSHDGKQLIFQSTRDGRSCDQQYIMNVDGSGVHRVSNGTGKTTCGYF
                     FAYDKRIFFASTHAADTACPPRPDPSKGYVWGLDPFDIYTANPDGSNLQRLTNYGVYT
                     AEGTLSPDGRTIVFTSLKDGDLDLYAMDVDGTHVRRLTDTPGYDGGAFFSPDGTKIVY
                     RANHPTDSTELAEYRALLAQRIVRPNKMELWVMNADGSGQHQITHLGGANFAPYFTPD
                     GRRIIFSSNYKNPHSGNFDLYLVNIDGSALEQITTDPGFDGFPQFSPDGRFLVWASSR
                     NAPDPHETNIFIARWAQANAAR"
     gene            complement(1100..>1670)
                     /locus_tag="VFU90_01905"
     CDS             complement(1100..>1670)
                     /locus_tag="VFU90_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478551.1"
                     /translation="QKNRKPANPDDLQGNASEAPPDSLEKESVKQNRPAYRSDAGRIV
                     YGGGGITPDVIIADDTITTPEQTFFKAIAPKYPAVRGVLYSYALQLKGQVSPKFTVKP
                     EWRDEFYRRLQAAKITVDRAQYDSAAPLINRWIGNQVAELAFGDSTEFRREIPDDKQL
                     QRAMELLRKGGTQKDLFSLAQGASPPVKQ"
BASE COUNT          275 a          499 c          553 g          343 t
ORIGIN      
        1 aatggcgagc gagaatgctc agcgggcggc gttcgcttga gcccagcgtg caatgaagat
       61 gttcgtttcg tgcgggtccg gcgcgttgcg gctcgacgcc caaaccagaa agcgtccatc
      121 cgggctgaac tgcgggaaac catcaaagcc cggatcggtg gtgatttgct ccagcgccga
      181 tccgtcgatg ttgacgagat acaagtcgaa attgccgctg tgcgggttct tgtagttgga
      241 tgagaagatg atccgacgcc cgtcgggtgt gaaatatggc gcgaagttgg cgccgcccaa
      301 gtgggtgatt tgatgctgac cggagccatc ggcgttcatc acccagagct ccatcttgtt
      361 cggtctgacg atgcgttggg cgagcagcgc ccgatattcg gccagctccg tcgaatcggt
      421 cgggtggttg gcgcgataga cgattttcgt tccgtcgggc gagaagaacg cgccgccgtc
      481 atagccgggc gtatcggtga gacgccgcac gtgcgtgccg tcgacgtcca tcgcatagag
      541 atccaggtcg ccatccttga gcgacgtgaa cacgatggtc cggccgtcgg gcgacaacgt
      601 gccttcggcg gtgtagacgc cgtagttcgt taggcgctgg agattggagc cgtcggggtt
      661 ggcggtgtag atgtcgaacg ggtcgagtcc ccacacgtag cctttggatg gatccggcct
      721 gggcgggcag gcggtgtccg ccgcgtgcgt cgacgcgaag aaaatgcgct tgtcgtatgc
      781 gaagaaatag ccgcacgtcg tcttgccggt gccgttcgac acgcgatgca cgccggagcc
      841 gtcgacgttc atgatgtact gctgatcgca gctccgcccg tcgcgtgtgg actggaagat
      901 cagctgcttt ccgtcgtggc tgaaatacgc ttcggcgttc gcgccgccat gcgtgagctg
      961 gcggatgtcc gcgaggcgcg gctcgttcgc gaccggcgct gccggcgcca cgttggcgtt
     1021 gggcgcggga tgcccacatg cgaccgcggc cacgagcgtg gccgcggcga ctctgtacgg
     1081 ggctctcatg cccccaaact tactgcttta ctggcgggct cgctccctgg gccagtgaga
     1141 acaggtcctt ctgcgtgccg cccttgcgca ggagctccat cgcacgctgc aactgcttgt
     1201 cgtccggaat ctcgcgacgg aactcggtcg aatcgccaaa cgcgagctcg gcgacctgat
     1261 tgccgatcca ccgattgatc agcggtgcgg cagaatcgta ttgcgcgcga tcgaccgtga
     1321 tcttggccgc ctgaagccgg cgatagaact catcacgcca ttcgggtttc accgtgaact
     1381 tcggcgaaac ctgtcccttg agctgcagcg catagctgta caacacgccg cgcaccgcgg
     1441 gatacttggg cgcgatcgcc ttgaagaacg tctgctccgg cgtcgtgatc gtgtcgtccg
     1501 cgatgatgac gtccggcgtg atgccgccgc caccgtacac aatgcgacct gcgtcgctgc
     1561 ggtatgccgg gcgattctgc ttcacagatt ccttctcgag cgagtctggc ggagcctcgc
     1621 tcgcgttccc ttggagatcg tccgggtttg ccggcttccg attcttctgg
//
LOCUS       DASUCJ010000152         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1208997, whole genome shotgun sequence.
ACCESSION   DASUCJ010000152 DASUCJ010000000
VERSION     DASUCJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208997"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFU90_01910"
     CDS             <1..582
                     /locus_tag="VFU90_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5478552.1"
                     /translation="KTLLPTPTNDGAGQWQLFFDDVVVPAGNIVGTEGNGAMAMFNSL
                     NPERVLAAAICSGMAHICLEKSVAYAKERRVFKDTPIGAYQSIAHPLAEVKILHEAVK
                     LMMYRAAWSFDQGRDPLEVGSQSNMAKYLAAEMAIKAVDAALETHGGNGFSEDFGLIN
                     LWFGARLFRTAPVSHEMILNYVSEWSLGLPRSY"
     gene            620..>1592
                     /locus_tag="VFU90_01915"
     CDS             620..>1592
                     /locus_tag="VFU90_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5478553.1"
                     /translation="MQLAEMDLYDPEIYAERVPHEEFAVLRREAPVYWHREADGPGFW
                     ALTKYEDIVTVSQDSQTFSSYRGGTNIDDVREDALAMIRTLMLNMDPPKHTVYRRLVA
                     TGFTPAMIRKMEPHVREVTARVIDSIAQKGECDFVTEVAAELPLQVIAAILGVPHDER
                     HLMFEWSNRLIGFDDPEFTTSPEDGRQAAAELLMYGHQLAGKRRQEPQDDLITTLLRA
                     EIDGQALSEAEFDAFFMLLVVAGNETTRNGISGAMRAFMEHPDQRQRLIDDPSMIPIA
                     VEEMLRWVSPVMHFRRTATRDAEIRGQKVREGDKVMIYYISANRDEDI"
BASE COUNT          290 a          489 c          579 g          234 t
ORIGIN      
        1 aagacgctgc tcccaacgcc cacgaacgac ggcgccggac agtggcaact cttcttcgat
       61 gacgtcgtcg tgcctgccgg caacatagtc gggaccgagg gcaacggcgc gatggcgatg
      121 ttcaactcgc tgaatccgga gcgcgtgctg gccgccgcga tctgctccgg catggcacac
      181 atttgcctgg agaagtcggt ggcgtacgcg aaggaacggc gcgtgttcaa ggacacgccg
      241 atcggcgcct accagtcgat cgcgcacccg ctcgcggaag tgaagatcct gcacgaagcc
      301 gtgaagctga tgatgtaccg tgcggcgtgg tcgttcgacc aggggcgcga tccgctcgag
      361 gtcggcagcc agtcgaacat ggcgaagtac ctggcggcgg agatggcgat caaggcggtc
      421 gacgcggcgc tcgagacgca tggcggcaac ggcttctcgg aggacttcgg gctgatcaac
      481 ctgtggttcg gcgcgcggct gttccggacg gcgccggtga gccacgagat gatcctgaac
      541 tacgtatcgg agtggagcct ggggctgccg cgctcgtact gatcgagcgg gcacgcccgg
      601 aaacggagca acggcaatgg tgcaactggc cgagatggac ctgtacgacc ccgagatcta
      661 cgcggagcgc gtgccgcacg aggagttcgc cgtgctgcgc cgcgaggcgc cggtgtactg
      721 gcaccgcgag gcggacggtc ccggcttctg ggcgctgacg aagtacgagg acatcgtcac
      781 ggtgtcgcag gactcgcaga cgttctcgtc gtaccgcggc ggcacgaaca tcgacgacgt
      841 gcgggaggac gcgctggcga tgatccgcac gctgatgctg aacatggacc cgccgaagca
      901 cacggtgtac cgccggctcg tcgcgacggg cttcacgccg gcgatgatcc ggaagatgga
      961 gccgcacgtg cgcgaggtga cggcgcgcgt catcgacagc atcgcccaga agggcgaatg
     1021 cgacttcgtg acggaggtcg cggcggagct gccgctgcag gtgatcgcgg cgatcctggg
     1081 cgtgccgcac gacgagcggc acctgatgtt cgagtggagc aaccggctga tcggcttcga
     1141 cgacccggag ttcacgacgt cgccggagga cgggcggcag gcggcggcgg agctgttgat
     1201 gtacgggcac cagctcgcgg gcaagcggcg gcaggagccg caggacgacc tgatcacgac
     1261 gctgctgcgc gcggagatag acggccaggc cctgagcgag gcggagttcg acgccttctt
     1321 catgctgctc gtcgtcgcgg gcaacgagac gacccgcaac ggcatctccg gggcgatgcg
     1381 cgcgttcatg gagcacccgg atcagcggca gcggctgatc gacgacccgt cgatgatccc
     1441 gatcgccgtc gaggagatgc tgcgctgggt gtcgccggtg atgcatttcc ggcgcaccgc
     1501 gacgcgcgac gccgagatcc gcggccagaa ggtccgcgaa ggtgacaagg tcatgatcta
     1561 ctacatctcg gcgaaccgcg acgaggacat ct
//
LOCUS       DASUCJ010000153         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_551818, whole genome shotgun sequence.
ACCESSION   DASUCJ010000153 DASUCJ010000000
VERSION     DASUCJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551818"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..635
                     /locus_tag="VFU90_01920"
     CDS             126..635
                     /locus_tag="VFU90_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478554.1"
                     /translation="MDTTITAPPIIFEPLFDDITPPTRATTGAAGYDVRAHLHTRAVR
                     TWRAGAEILEPASTDGVFTLEPGDVALIPLGFRARLPAGYEAQVRLRSSIAFRRGLIL
                     PNAPGTIDADYPDEWLVMVRNVSTHRVAIEHGERIAQIILHRYASLAWTVGVVTQSTD
                     RRGGIGSTG"
     gene            756..>1004
                     /locus_tag="VFU90_01925"
     CDS             756..>1004
                     /locus_tag="VFU90_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478555.1"
                     /translation="MIHLVGLGHAVITVGVTRLTQASETLFASALLFVSEAGRPIERH
                     RIIELLWPRTSAQRASHRLRQTLYRLNALGASLRSDRSH"
BASE COUNT          182 a          373 c          294 g          155 t
ORIGIN      
        1 atggcggccg gcgacgcagg cgccccagcg ggacgcgacc cggcgccggc aggcggcgtg
       61 acggtcacga gcaaaaccta tagtccgtgc cggccacggc caagcacgca aatcaacgag
      121 cgttcatgga taccacgatc accgcgccgc ccatcatctt cgagccgctc ttcgatgaca
      181 tcacgccgcc gacgcgcgcc acgacgggcg ccgccggcta cgatgtgcgc gcccatctgc
      241 acacccgcgc cgttcgcaca tggcgagcgg gcgcagagat cctcgagccg gcaagtaccg
      301 acggagtgtt cacgctcgag cccggcgacg tcgcactcat tccgttgggc ttccgtgccc
      361 ggctgccggc cggctacgag gcgcaggttc gactgcggag ctcgatcgcg tttcgccgcg
      421 gcctcattct gcccaacgca cccggcacca tcgatgccga ctatcccgat gaatggctcg
      481 tgatggtgcg caatgtttcc acgcaccgcg tcgccatcga acacggtgag cgcatcgcgc
      541 agatcatcct gcatcgctac gcatcgctcg cgtggaccgt cggcgtcgtc acgcagtcca
      601 ccgatcgccg cggcggcatc ggcagcaccg gctgacgaac tcgccgctcc cgcctaaccg
      661 aatcgtatga cactgtagta cgaatgacgc tcgcgtgacg agctcggcgt tagtaccttg
      721 gtcgggcgaa aaaacacgcc acggaaacgc gacacatgat ccacttggtc ggactcggac
      781 acgccgtcat cacggtcggc gtcacgcgcc ttacccaggc cagcgaaacc ctctttgctt
      841 ccgcactgct gttcgtatcc gaggcaggcc ggcccatcga gcggcatcgc atcatcgagc
      901 tgctctggcc gcggacatcg gcccaacgcg ccagtcaccg gctcagacag accctctacc
      961 ggctcaacgc actcggcgct tcactccgct cggatcgcag ccac
//
LOCUS       DASUCJ010000154         1376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_184875, whole genome shotgun sequence.
ACCESSION   DASUCJ010000154 DASUCJ010000000
VERSION     DASUCJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184875"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /gene="rho"
                     /locus_tag="VFU90_01930"
     CDS             complement(<1..616)
                     /gene="rho"
                     /locus_tag="VFU90_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5478556.1"
                     /translation="MRRADDSYLPMPGDPFVAPFLVRQFALRRGDLIEATTGRDHRGR
                     TTLVELTHVNGEDPASAQRRPDFSSLIASYPERKLVLETGRSAKGGPELTRRAIDLIA
                     PIGYGQRALIVAPARAGKTMLLQAITEGIAINHPEAVLLVLLVDERPEEVSEMISWGR
                     GEVVASSFDQPAERHVDVADMVLERSRRLVESGKDVVIVLDSLTR"
BASE COUNT          218 a          459 c          443 g          256 t
ORIGIN      
        1 gccgcgtcag ggagtcgagt acgatcacga cgtctttgcc gctttcgacc aggcgccgcg
       61 aacgctccaa caccatatcc gcgacatcga cgtgccgctc agccggctga tcgaagctcg
      121 atgccacgac ttcaccgcga ccccacgaga tcatctcgct cacttcttcc gggcgctcgt
      181 ccacgagcag cacgagcagc accgcttccg gatgattgat cgcgatgcct tcggtgatcg
      241 cctggagcaa catcgtcttg ccggcgcgcg ccggtgcgac gatcaaggcg cgttgtccgt
      301 agccgatcgg cgcgatgaga tcgatggcac gacgcgtgag ctcggggcct cctttcgccg
      361 aacgtcccgt ctcgaggacc agctttcgct ccgggtacga tgcgatcagc gaactgaaat
      421 ccggacgacg ttgggcgctg gctggatctt cgccgttcac atgcgtcagc tcgactaacg
      481 tcgtgcggcc gcgatggtcg cggcccgtcg ttgcctcgat cagatcgccg cggcgcagtg
      541 cgaattgccg caccaggaac ggcgccacga acgggtcgcc cggcatcggg aggtagctgt
      601 catccgcgcg tcgcacgaag ccgccttcgc gcgaagaatc aaaccaaccc gtgacggtgc
      661 cgtccgccac gacaggctgc ggcgggccac cgttgtgatg atcgggacgc ggaccactcc
      721 gtccgcgacc acgtctgcct cgccttccat tggtggggaa tcgtccgtcg cgctgaccct
      781 ggtgctgacc accgccgcct ccttgattga cgccaggcgt cgatccaccg gtgaatcccg
      841 gcgcaggcga cgatccgggc ggcgtgttcg acggaggcgc gttgggcacc gcgtcattcg
      901 cgggttcggg agccgaaccg ccggtcgagt caccactcgg cgcctctccg ttagacacag
      961 ggaatgccgt cggttcaccg ttcatgggga gtccaacatc ctcgcgatat ggactgggtt
     1021 cggattgcgt gtcgcggggt ccgccgccgc ggttctgcgg gcgtggaccg cgaccgcgcc
     1081 gtgtggggcg tcgacgatcg ttcatgcacc aatgcgtgtc atggagtgat tggatggcgc
     1141 ttgtggcgcc caccgatgtg gggccgctac gctgctgcta cgtctctcgc gagatgcacg
     1201 tatccggaaa ctcatccggc gtcatgcgat ctcgacgttt gtatggagct cggtctatcg
     1261 gcggatccga actggcatca cagtcggcgc gctcgagcgc gacacacgac gtcgtgattc
     1321 tgcctgtccc gcggtgctcc taacgcgctc ttcgcgacgt ctggaaaccg cggatg
//
LOCUS       DASUCJ010000155         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_460161, whole genome shotgun sequence.
ACCESSION   DASUCJ010000155 DASUCJ010000000
VERSION     DASUCJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460161"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFU90_01935"
     CDS             complement(<1..362)
                     /locus_tag="VFU90_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478557.1"
                     /translation="MSISNRSCCALGLLIVLLAGCGGPGSLGSPSRSLGLALERKRDR
                     GSWMSEAAASGATTLLYAGDANGDTVYVYDYTSGALVGTLAGFNEPGPGCVDRRGNVF
                     ITNYGNGKVLEFRHGGTR"
     gene            complement(402..>1281)
                     /locus_tag="VFU90_01940"
     CDS             complement(402..>1281)
                     /locus_tag="VFU90_01940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5478558.1"
                     /translation="SKDTLKIDPFLPACIGSPNCSAAVTGSLPSNFVPPLTGKPLLVQ
                     AVRIVQPANYARTVLVEMLRAAGVTVAAPVVEANPVRLLPAKNSYRRDARVAQLTGMQ
                     YGQDAKLILKISYNIGADTSLVLYGLTRGVNSMPAALAVERKTLASQFGVQPSEYHFV
                     DGSGGGETTATGRAVTHMLDGLAKSPAHATFYDDLPILSVDGSLAFVKDFQKDPSLKG
                     AAGQVHAKTGTYVNGSAEGTMLKGQALAGYITTKNGRHLTFELVVNEMPLSGIMELTK
                     VFQDQGTIAAILWRDY"
BASE COUNT          219 a          404 c          423 g          235 t
ORIGIN      
        1 aacctcgtgc cgccgtgccg aaactccaga accttcccgt tgccgtagtt cgtgatgaag
       61 acgttgccgc gccgatcgac gcagccgggg ccgggctcgt tgaatccggc gagcgtcccc
      121 accagcgctc cgctcgtgta gtcgtatacg tacacggtat cgccgttcgc gtcgccggcg
      181 taaaggagcg tcgtcgcacc cgacgccgcc gcttcgctca tccacgatcc gcgatcgcgc
      241 ttccgttcga gcgcgagccc gagggaacgt gacggcgaac cgagcgaacc cggaccaccg
      301 caaccggcca gcagtacgat gagaagtccg agcgcgcagc agctgcgatt ggagatgctc
      361 atgccggttg gcgaactata gcgtggccgg acgtttccgc tctagtagtc ccgccacagg
      421 atggcggcga tcgtcccttg atcttggaac accttcgtga gctccatgat gccggagagc
      481 ggcatctcgt tcaccacgag ctcgaaggtc aagtgacggc cgtttttcgt ggtgatgtag
      541 cccgcgagcg cctggccttt gagcatcgtg ccttcggccg aaccgttgac gtaggtcccg
      601 gtcttggcgt gcacttgacc ggccgcgccc ttgagcgacg gatctttctg gaagtccttg
      661 acgaaggcga gcgatccgtc gaccgaaagg atcggcagat cgtcgtaaaa cgtcgcgtgt
      721 gccggcgact tggcgaggcc gtcgagcatg tgcgtgaccg cgcggcccgt cgcggtggtc
      781 tcgccgccgc cgctgccgtc gacgaagtga tattccgacg gctgaacgcc gaactgcgac
      841 gcaagcgtct tgcgctcgac cgcgagcgca gcgggcatcg agttcacgcc gcgcgtcaat
      901 ccgtagagca cgaggctggt gtcggcaccg atgttgtagc tgatctttag gatcagtttc
      961 gcatcctgcc cgtattgcat gccagtgagc tgcgcgaccc gcgcatcgcg ccggtacgaa
     1021 ttcttggccg gcagcaggcg cacgggattc gcttcgacga ccggtgcggc aacggtcacg
     1081 ccggcggcgc gcagcatttc gaccaggacg gtgcgggcat agttggccgg ctgcacgatc
     1141 cgtacggctt gtaccagcag cggcttgccg gtgagcggcg gaacgaaatt gctcggcagg
     1201 ctgccggtga ccgcggcact gcagttcggc gaaccgatac aggccggtaa gaacgggtcg
     1261 atcttgagcg tgtctttgga a
//
LOCUS       DASUCJ010000156         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_62611, whole genome shotgun sequence.
ACCESSION   DASUCJ010000156 DASUCJ010000000
VERSION     DASUCJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62611"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFU90_01945"
     CDS             <1..859
                     /locus_tag="VFU90_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU5478559.1"
                     /translation="MGSDRFYPEEAPVRDVRVEGFAIDPYAVTNEQFSKFVRKTGYVT
                     VAERPLDAADYPGAPAENLVPGALVFHMTAGPVDYRDFTQWWSWVPGACWKHPDGPGS
                     SIDGRPDHPVVHVAFEDADAYARWAGKRLPTEAEWEFAARGGLDGKDFVWGDEMEPGG
                     KPAANTWQGEFPWKSTARNGARTMPVGRFAPNGYGLYDMAGNVWEWTADWYAADRSAV
                     ESPCCAGDARERSFDPCQPASRIPRKVLKGGSFLCSPSYCLRFRPAARSPQAIDTGMS
                     HVGFRCVAD"
     gene            890..1735
                     /locus_tag="VFU90_01950"
     CDS             890..1735
                     /locus_tag="VFU90_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478560.1"
                     /translation="MSKTVENERSTPLVTHREFKLLLKPEKFPNRRALLEFNELLQKT
                     AAKLGVDYEPVESLDSQLRIVQFYDSKTEVLRKNKLIFRIRQIRQGGWPDESWELTFK
                     CRAPEYEKAAAFDTDTTMTKLQKKKFKEELIRGEAPGTMVSIFSNNVIVEYPEINPEF
                     PISRLAEALPHLKTLDLDMNEPLSIVAGAKVFEIQSTLGTLSFGHGVSASATLAVWAR
                     PVPDRFEPLIVEFGFSTHVRGKDKEHLGQDAADEFFKALQVPLREYLADGTTKTALIY
                     GDEGA"
     gene            1722..>2383
                     /locus_tag="VFU90_01955"
     CDS             1722..>2383
                     /locus_tag="VFU90_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5478561.1"
                     /translation="MKEPSVADEAPLSGAGRVPKLREYANAFGPGLVSGASANDPTTV
                     ASLAVVGATTVYGLSWLVILLLPMLVLVQTIATSIAAVAQMSLQEAVVMKYGRPVALA
                     GVVAVALVGLLVLAADVKAGAEALTLITGVPLYVFVLPLVAAAGWLLATNSYLKIERI
                     LASFTLIFLCYAASAVYAHPNWGEVLRSILVPHFEFSRPFATGALALLGTTLTSFTYF
                     WQS"
BASE COUNT          435 a          734 c          776 g          438 t
ORIGIN      
        1 catgggctcc gaccgattct acccggagga ggcgcccgtg cgtgacgttc gcgtcgaagg
       61 cttcgcgatc gacccctacg cggtcaccaa cgaacagttt tcgaagttcg tgcgcaagac
      121 cggatacgtc acggtggccg aacggccgct cgacgccgcc gattatccgg gcgctccggc
      181 ggagaatctc gttccgggcg cgctggtgtt ccatatgacg gccggtccgg tggattatcg
      241 cgacttcacc caatggtggt cgtgggtgcc gggtgcgtgc tggaagcatc ccgacggccc
      301 gggcagctcg atcgacggac gccccgatca cccggtcgta cacgtcgctt tcgaagatgc
      361 cgacgcatac gcgcgctggg ccggcaaacg gttgccgacg gaagccgaat gggagttcgc
      421 ggcgcgcggc ggtttggacg gcaaagactt cgtttggggc gacgagatgg aacccggcgg
      481 caaaccggcc gccaatacgt ggcagggtga gttcccgtgg aaaagcaccg cccggaacgg
      541 cgcccggacg atgcccgtcg ggcgcttcgc ccccaacggc tacggtttgt acgacatggc
      601 cgggaacgtg tgggagtgga cggcagattg gtatgccgcc gaccggagcg cggtcgaatc
      661 gccgtgctgc gccggggacg cgcgcgagcg cagtttcgac ccgtgccagc ccgcgagccg
      721 cattccgcgc aaagttctca aaggcggatc cttcctgtgt tcgcctagtt actgtttgcg
      781 ttttcggccg gcggcgcggt cgccgcaggc gatcgacacg gggatgtcgc acgtcggctt
      841 tcgctgcgtc gccgattgaa cgaaactcgc cgagaaaggt aggacagcga tgagcaaaac
      901 cgtcgagaac gagcgctcga ccccgttggt gacccaccgc gaattcaagc tgctgctcaa
      961 gcccgagaag tttcccaacc ggcgagctct gctcgagttc aacgagctgc tgcaaaaaac
     1021 ggcggccaaa ctgggcgtcg actacgagcc ggtcgaatcg ctcgactcgc agctgcggat
     1081 cgtgcagttc tacgacagca aaaccgaagt cttacgcaag aacaagctga tctttcggat
     1141 ccgccagatt cgccagggcg gctggcccga cgagagctgg gagctgacgt tcaaatgccg
     1201 cgcgcccgag tacgagaaag cggcggcctt cgacaccgat acgacgatga ccaagctgca
     1261 aaagaagaag ttcaaggaag aactcattcg cggcgaagcg cccggaacga tggtcagcat
     1321 tttttccaac aacgtcatcg tcgaatatcc cgagatcaat ccggagtttc cgatatcgcg
     1381 actggccgag gcgctgccgc atctcaagac cctcgatctc gatatgaacg agccgctgtc
     1441 gatcgtagcc ggggcgaaag tgttcgagat ccaaagcacg ctggggacgc tttcgttcgg
     1501 tcacggggtt tccgccagcg ccacgctggc cgtttgggcg cgcccggtgc cggatcgctt
     1561 cgagcccttg atcgtcgagt tcgggttttc gacgcacgtg cgcggcaagg ataaggagca
     1621 tctcggccaa gacgcagccg acgagttctt taaggcactg caggtgccgc tgcgagagta
     1681 tctcgccgac ggtaccacca agactgccct catctatggg gatgaaggag cctagcgtag
     1741 ccgacgaagc gccgctttcc ggagccggac gcgtgccgaa actgcgcgag tacgccaacg
     1801 cgttcggacc cggcttggta tcgggggcgt cggcgaacga tccgaccacc gtcgcgtcgc
     1861 tcgcagtcgt cggagccacg acggtctatg gactgtcgtg gctcgtcatt ctgctgctgc
     1921 cgatgctggt gctcgtccag acgatcgcca cgtcgatcgc cgcggtcgcg cagatgagcc
     1981 ttcaggaagc ggtggtgatg aagtacgggc gtccggtcgc gctcgccggc gtggttgcgg
     2041 tggcgctcgt cggcctgctg gtcctcgccg ccgacgtgaa agcgggcgcg gaagcgctca
     2101 cgctgattac gggcgtaccg ctgtacgttt tcgtgctgcc gctggtcgct gcggcgggct
     2161 ggctgctggc gacgaattcg tacttgaaaa tcgagcgaat cctcgcgtcg ttcaccttga
     2221 tcttcctatg ttacgccgcg agcgccgtct acgcgcatcc taattggggt gaagttctgc
     2281 gcagcatcct cgtcccgcat ttcgaatttt cgcggccgtt tgcgaccgga gcgctcgcac
     2341 tgctcggcac gacgctcacg agtttcacgt atttctggca atc
//
LOCUS       DASUCJ010000157         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_903290, whole genome shotgun sequence.
ACCESSION   DASUCJ010000157 DASUCJ010000000
VERSION     DASUCJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903290"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..991
                     /locus_tag="VFU90_01960"
     CDS             566..991
                     /locus_tag="VFU90_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5478562.1"
                     /translation="MNIKLATAVFVAGAMLVPAATWAAGSAGAPGAPAAPAQESGSMM
                     NKAKGAVSDTTITAKVKARLSSDRDMHGNDITVSTEYGTVNLGGTAKSEQDAQKATEI
                     AKATEGVASVNNDIKVDSGGAMGGAAGGATGGATGGAKY"
     gene            complement(1071..1439)
                     /locus_tag="VFU90_01965"
     CDS             complement(1071..1439)
                     /locus_tag="VFU90_01965"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEU5478563.1"
                     /translation="MNRRREFTMRCFESGTVVSLAPREVVTLPDARGAVMRVMQGTLW
                     VTQHGSAQDVVLRGGDNWVVERNGATVVEAQNDAVFCVAGKPGAEFQRAVAGPRSRAR
                     VGTALKRWLHATRRAPVPYF"
     gene            1577..>2386
                     /locus_tag="VFU90_01970"
     CDS             1577..>2386
                     /locus_tag="VFU90_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5478564.1"
                     /translation="MHRTLRALPSLDFLRGFEAVGRRLSFTQAADELFVTQSALSRQV
                     KALEDALGVALFERQHRALALTVAGAAYHRSVTDILEALASATERTRDSSRAPGLTVS
                     TTVSFASLWLIPRLARYRALEPAAEVYVSADDRVVDLAHGDVDAVIRYLPDSAAPPGA
                     LRLFGDRLTPVATAAIVRGKSPLRTPADLARHVLLHFDDRDGRMPWLNWPSWLASNGA
                     GDLKPAGSLHFRLYDQVIQAAVGGQGVALGRLPMLGEHLRDGRLVAPFARKL"
BASE COUNT          397 a          773 c          827 g          389 t
ORIGIN      
        1 ctgcggccgc cgcggctgca gtcgacgtct tcagtgcgtt cattgcggat ctcctctggt
       61 agctgggtgg tgacgcgacg cacgatgcga ttcgcgggtg cggctccccc ccacgatccc
      121 ggcaatccag gggaggatcc atcgacaccg cgcggcgcga ttctgcgcgc cgccctggcg
      181 ctccgcaatg ggcgcgtgaa tacgtgccga cgtccgcgcg aagggcggtg cgattgcgtt
      241 cgcgaagaga cgcgcgaccg gttcgctgcg taacgatgca cgcgcgtagg agtacgcgcg
      301 agcatcgcga attccgaccg gcgaatcggt cgatgtccta cgccagcgcg cgccatggcg
      361 cggaacccgt gtgcggtgat gcgtaccaaa cgggatgccg caccgattga cgcaccggat
      421 cgatcggatg gctcgcggca gccatcgcgt gcgctggatc gcagcatgca atcgaggcgt
      481 cgatgagccg acaggatcgt cgtcgatcga cgtcagctgc cgatcccgcg caattgtccg
      541 gaaacattgc aaggagtacg caatcatgaa catcaaactg gccacagcag tctttgtcgc
      601 aggcgcaatg ctggtgccgg cggcaacctg ggcagccgga tcagcgggag cgccgggtgc
      661 acccgctgcg cccgcgcagg aaagcggatc gatgatgaac aaggcgaagg gtgcagtgag
      721 cgacacgacg atcaccgcca aggtgaaggc caggctgtcg tcggaccgcg acatgcacgg
      781 aaacgacatt accgtcagca ccgagtacgg tacggtcaat ctcggcggta cggcgaagtc
      841 cgagcaggat gcccagaagg caaccgagat cgccaaagcc acggaaggcg tggcgtcggt
      901 caacaacgac atcaaggtcg attccggagg tgcgatggga ggcgcggccg gaggtgcgac
      961 cggaggcgcg accggaggcg cgaagtactg accctcggat attgaacgag aatggggcgg
     1021 cggatgccgc cccattgtgt atcggcctcg ggaacaccgg agccgacgag tcagaagtac
     1081 ggaaccggcg cccgccgagt cgcgtgcagc cagcgcttca gcgccgtgcc gacgcgggcg
     1141 cggcttcgcg ggcccgcgac ggcacgctgg aattcggcgc caggtttccc ggcgacgcag
     1201 aataccgcgt cgttctgcgc ctcgacgacg gtggcgccgt tgcgctcgac gacccagttg
     1261 tcgccgccgc gaaggacgac gtcctgcgca cttccgtgct gcgtgaccca cagcgttccc
     1321 tgcatcaccc gcatgacggc gccgcgagcg tctggtagcg tcacgacctc gcgcggcgcg
     1381 agcgagacaa cggttcccga ttcgaagcat ctcatggtga actcccgtcg acgattcatc
     1441 gtgtaatcta tgcgtgtgat tcatcgctac agaatccgcg cacgtgtgtg cgggcgctga
     1501 tccatgagag gaattgtctg tggcggagcg cggtacggca aacgatcatt cggaatcatt
     1561 cgcatgagct gcgagcatgc ataggacgct gcgcgcgtta ccgtcgctcg acttcctgcg
     1621 cggcttcgag gcggtcggcc ggaggctctc gttcacgcag gctgccgacg agcttttcgt
     1681 gacgcagtcg gcgttgtcgc ggcaagtgaa ggcgctcgag gatgcgctcg gtgtcgcgct
     1741 gttcgagcgg cagcaccgcg cgcttgcgct gacggtcgcc ggggcggcat atcaccgctc
     1801 ggtgaccgac atcctggagg cgctggcctc ggcgaccgag cgcacacgtg actcgagccg
     1861 cgcgccgggg ctgaccgtgt cgacgacggt ctcgttcgcg tcgctgtggc tgattccgcg
     1921 tctcgcacgc taccgcgcgc tcgaaccggc tgccgaggtc tacgtgtcgg ccgacgaccg
     1981 cgtcgtcgat ctcgcgcacg gggacgtcga cgcggtgatt cgctaccttc ccgattcggc
     2041 ggcgccgccg ggggcgctgc ggctcttcgg cgatcgcctc actccggtgg cgactgcggc
     2101 gatcgtcagg ggaaagtctc cgctgcgcac gcctgccgac ctcgcgcggc acgtgctcct
     2161 tcatttcgac gatcgcgacg gccggatgcc gtggctcaac tggccgagct ggctcgcgtc
     2221 gaacggggcc ggtgacctga agcccgccgg ctctcttcac tttcgtcttt acgaccaggt
     2281 aatccaggcc gcggtcggcg ggcagggcgt cgcactcggc cgcctgccga tgctcggcga
     2341 gcacctgcgt gacgggcggc tggtcgcgcc gttcgcgcgc aagctc
//
LOCUS       DASUCJ010000158         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_123929, whole genome shotgun sequence.
ACCESSION   DASUCJ010000158 DASUCJ010000000
VERSION     DASUCJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123929"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VFU90_01975"
     CDS             complement(<1..1255)
                     /locus_tag="VFU90_01975"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5478565.1"
                     /translation="MNPPAPVPTLMPNEKHPDVMDRLVSLCKRRGFIFQSSEIYGGTG
                     SVWDYGPLGVELKKNIKDRWWHAMVRARDDIEGLDAAILMHPRVWEASGHVAGFVDPL
                     VDCRNCKKRFRADDPRIKGEPGTPDAQCPSCGMKGTLSAPRQFNLMFKTYMGPVEDTA
                     SIVYLRPETAQGIYVNFVNVQQSTRQKIPFGIAQIGKAFRNEITPGNFIFRTREFEQM
                     EMQFFVEPGTDMTWFEYWKEQRMQWHLSLGLDRTRLQFHQHTAEELAHYARAAFDVQL
                     DFGGTLGFQEIEGVHNRGDFDLTRHAESSGKKLEYVDQPNNRRFTPYVVETSVGADRT
                     TLAVLVNGYREESVPGESEGRTVLAIHPSLAPVKAGIFPLVKKDGMPEMAARIAADLR
                     PWFPVFYDEAGAIGRRYRRQDEVGTP"
     gene            complement(1252..2232)
                     /locus_tag="VFU90_01980"
     CDS             complement(1252..2232)
                     /locus_tag="VFU90_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEU5478566.1"
                     /translation="MAERLDAVIIGAGPCGLAAAVAFKRAGLRAVVIDRSCVVSGIAG
                     YPTYITFFSTPDRLSIGGVPFIVATEKPTRRDALAYYRAVTTMFDLDVRQYENVERIR
                     RCDDAFAIESRTRHGVRRETRARAVVIATGYFGHPNRLDVPGEDLPHVTHLFTEGHWA
                     FRQDVVVVGGGNSAVEAALDLYRSGARATIVHFLPQLDHNIKPWVLPDITNRIKDGSI
                     GARFSSRVRAITPEHVELDTPDGVDRVRADHVYLMVGYQPNAELLVQLEVPIDARTGI
                     PAHDPATMETAVPGVFIAGVIASGNDANKTFIENGRFHGDLIAGRLAEMP"
BASE COUNT          382 a          807 c          812 g          415 t
ORIGIN      
        1 acggcgtacc gacttcatcc tggcgacgat agcggcgccc gatggcgccc gcctcatcgt
       61 agaacaccgg gaaccacggg cgcaggtccg cggcgatacg cgccgccatc tcgggcatcc
      121 catccttttt gactaacggg aagattccgg ccttgaccgg cgccagcgac ggatggatgg
      181 cgagcacggt gcgtccttcc gattcgccgg ggaccgactc ctcgcggtag ccgttgacga
      241 gcacggccag cgtcgtgcga tcggcgccga ccgacgtttc gacgacgtag ggcgtgaagc
      301 gccggttgtt gggctggtcc acgtactcga gcttcttgcc cgaagactcg gcgtgccggg
      361 tgaggtcgaa gtcgccgcgg ttgtggacgc cttcgatctc ctggaagccc aacgtaccgc
      421 cgaaatcgag ctggacgtcg aaggccgcgc gcgcgtaatg cgcgagctcc tccgccgtgt
      481 gctgatggaa ctggagccgc gtccggtcga ggcctaacga aaggtgccac tgcatccgct
      541 gctccttcca gtactcgaac cacgtcatgt ccgtgcccgg ctcgacgaag aactgcatct
      601 ccatctgctc gaactcgcgc gtccggaaaa tgaaattccc gggcgtgatc tcgttgcgaa
      661 acgccttgcc gatctgcgcg atgccgaacg ggatcttctg ccgcgtcgat tgctgcacgt
      721 tgacgaagtt gacgtagatg ccctgcgcgg tttccggccg caggtacacg atggatgccg
      781 tgtcttcgac cggccccatg tacgtcttga acatcagatt gaactggcgc ggtgcgctga
      841 gcgtgccctt catgccgcac gacgggcact gggcatccgg tgtgccgggt tcgcccttga
      901 tgcgcgggtc gtcggcgcgg aagcgcttct tgcagttgcg gcagtcgacg agcgggtcga
      961 cgaagccggc cacgtggccg ctggcttccc agacgcgcgg gtgcatcagg atcgcggcgt
     1021 cgagtccctc gatgtcgtcg cgggcgcgga ccatggcgtg ccaccagcgg tccttgatgt
     1081 tcttcttgag ctcgacgcct aacggtccgt agtcccacac cgagccggtg ccgccgtaga
     1141 tctcggacga ctggaagatg aacccgcgcc gcttgcacag cgacacgagg cgatccatca
     1201 cgtcgggatg tttttcgtta ggcatgagcg tcgggacggg agcgggcgga ttcacggcat
     1261 ctccgccagg cggcccgcga tcagatcgcc gtggaagcgg ccgttctcga tgaacgtctt
     1321 gttggcgtcg ttgccggagg cgatcacccc ggcgatgaac acgccgggca cggccgtctc
     1381 catggtggcc ggatcgtgcg ccgggatgcc ggtcctggcg tcgatcggca cctcgagctg
     1441 caccagcagc tctgcgttgg gctgatagcc caccatgagg tacacgtggt cggcgcgcac
     1501 gcggtccacg ccgtcgggcg tgtcgagctc cacgtgctcg ggcgtgatcg cgcgtacgcg
     1561 agacgagaac cgcgcgccga tgctgccgtc cttgatgcgg ttcgtgatgt ccggcagaac
     1621 ccacggcttg atgttgtggt ccagctgcgg caggaagtgc acgatcgtcg cgcgggcgcc
     1681 ggaccgatag aggtcgagcg ccgcttccac cgccgagttg ccgccgccga ccacgacgac
     1741 gtcctgacgg aacgcccaat ggccctcggt gaagaggtgc gtgacgtgcg gcagatcctc
     1801 gcccggcacg tcgaggcgat tgggatggcc gaagtagccg gtcgcgatca cgacggcgcg
     1861 cgctcgcgtc tcccggcgca cgccgtggcg cgtccgcgat tcgatcgcga acgcatcgtc
     1921 gcagcgccgg atgcgctcga cgttctcgta ctgcctgacg tcgaggtcga acatcgtcgt
     1981 cacggcgcga tagtaggcga gcgcatcgcg gcgcgtcggc ttctcggtgg cgacgatgaa
     2041 cggcaccccg ccaatcgaca accgatcggg cgtcgagaaa aacgtgatgt acgtgggata
     2101 gccggcgatg ccgctcacga cgcacgagcg atcgatgacg accgctcgga gtcccgcccg
     2161 cttgaaggcg accgccgccg cgagcccgca aggcccggcg ccgatgatca ctgcatcgag
     2221 ccgctccgcc acgtccactg ctccgatcac tcgacgccga tgatctggtc gcggcccgtc
     2281 cccacgctca cgtacgcgat cggcgtgtcc gcgagctcgc ggatgcggtc caggtagcgg
     2341 cgcgcgggcg ccggcagatc ttccacgcgg cgcgcgcccg ccgtgctgct cctccagcct
     2401 tcgaaccact cgtagc
//
LOCUS       DASUCJ010000159         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_521181, whole genome shotgun sequence.
ACCESSION   DASUCJ010000159 DASUCJ010000000
VERSION     DASUCJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521181"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFU90_01985"
     CDS             complement(<1..466)
                     /locus_tag="VFU90_01985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5478567.1"
                     /translation="MNQQVKIVAAFVLLGGFFYGGWRLFVHQSNVMPALVTPEMNVHN
                     EIADDLPARAYRVTQESAPEDRSNRPKLIALTFDDGPYPVFTPMLLDVLRDERVPATF
                     FLIGRDAEQWPELTHRIVQDGHEVADHTYTHPDLDREPDDAVRAEILQGRDAL"
     gene            complement(471..>1824)
                     /locus_tag="VFU90_01990"
     CDS             complement(471..>1824)
                     /locus_tag="VFU90_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5478568.1"
                     /translation="NLWQRAGIVAGLVALGIGSTLAAYAFFEHKNPVTAITQIFVPTP
                     QQVFGKPNVLVLVEGLDYDYTAKDEEFSTNSRSDVIWAVNLDFAGKRIYELSIPRDMI
                     ATMPNGAKAKINTAQSAGGVTEAKSVIANFLGIPGFDRYVILRIDATKEFVNAIGGVN
                     LDVKSSDCLQHKSNCTGESLDYDDTWGHLHIHLKEGPQHLNGDQAVAYMRFRHDWCSD
                     PCRIMRQQQVLHAMATKLRSDRVNTMMHLGDLLNVFRKYVQTDFTTSELLSLATHYQG
                     ISDSEIIAKQVPYTDDIDLPPYGDSLVPDVEARSRLVESMLLAPPVPEPSPDAMALAA
                     IAPATLRIDVENGSGIPGAGRRVAQMLKKAGFTIGDVGNAPRSDYVSSEIHEHSMVTF
                     AGARVRAALPASLQKAAVVPDPSPSPEAEATATTSDVTVIVGSDLANAPASTSGQPSQ
                     "
BASE COUNT          304 a          571 c          598 g          351 t
ORIGIN      
        1 aaagtgcatc gcggccctgc agtatctccg cgcgcaccgc gtcgtcgggc tcgcgatcga
       61 gatcgggatg cgtgtacgtg tgatcggcca cctcgtggcc gtcttgcacg atccggtgcg
      121 tcaactccgg ccattgctcg gcatcgcgac cgatcagaaa gaaggtcgcc ggaacgcgtt
      181 cgtcgcgcag cacgtcgagc agcatcggcg tgaacaccgg gtacggaccg tcgtcgaacg
      241 tcagcgcgat cagtttcggg cggttcgaac gatcctccgg cgcggactcc tgcgtcacgc
      301 ggtacgcgcg cgccggcaag tcgtcggcga tctcgttgtg gacgttcatc tccggcgtca
      361 ccagtgcggg catcacgttg ctctgatgca cgaacagacg ccagcccccg taaaagaatc
      421 ctcccaggag cacgaacgcg gcgacgatct tcacctgttg attcacttcc ttactgactg
      481 ggttgccctg acgtgctcgc cggcgcattg gccaagtcgc tgccgacgat gacggtgacg
      541 tcgctcgtgg tggcggttgc ctcggcctcg ggcgaaggcg acgggtccgg gacgacggcg
      601 gccttctgca ggctcgcggg caacgcggcg cggacgcgcg cgcccgcaaa ggtgaccatc
      661 gagtgttcgt ggatctcgct ggaaacgtaa tcggaacgcg gtgcgtttcc gacgtcgccg
      721 atcgtgaagc cggccttctt gagcatctga gccacccggc gacccgcgcc gggaatgccg
      781 ctgccgttct cgacgtcgat ccgcagcgtc gcgggcgcaa ttgcggccag cgccatcgcg
      841 tccggcgaag gctcgggaac gggcggcgcg agcagcatcg attcgaccag ccggctgcgt
      901 gcctccacat cgggaacgag cgaatcgccg tagggcggca gatcgatgtc gtcggtgtac
      961 ggcacctgtt tcgcgatgat ttcgctatcg gagatgccct gatagtgcgt cgccagcgag
     1021 agcagctccg aggtcgtgaa atcggtctgc acgtacttgc ggaagacgtt cagcaggtcg
     1081 ccaaggtgca tcatggtgtt cacccggtcg ctgcgcagtt tggtcgccat cgcgtggagc
     1141 acttgctgct ggcgcatgat ccggcaggga tcgctgcacc agtcgtggcg gaaccgcatg
     1201 tacgcgaccg cctgatcgcc gttgaggtgc tgcggccctt cctttaagtg gatgtgcagg
     1261 tgaccccagg tgtcgtcgta atcgaggctt tcgcccgtgc agttcgactt gtgttgcagg
     1321 cagtcggacg atttgacgtc gaggttgacg ccgccgatcg cgttgacgaa ctccttggtc
     1381 gcatcgatcc gcaggatcac gtagcggtcg aagccgggaa tcccgaggaa gttcgcgatg
     1441 acgctcttgg cctcggtgac gccgccggcc gactgggccg tgttgatctt cgctttcgcg
     1501 ccgttgggca tcgtagcgat catgtcgcgc ggaatcgaca gctcgtagat gcgcttgccc
     1561 gcgaaatcga gattcaccgc ccaaatcacg tcgctgcgcg agttggtgga aaactcttcg
     1621 tctttcgccg tatagtcgta gtccaatccc tcgaccagca ccaagacgtt cggctttcca
     1681 aaaacctgct gcggcgtcgg cacgaagatc tgcgtgatcg cggtgaccgg gttcttgtgc
     1741 tcgaagaacg cgtaggccgc cagcgtcgac ccgatgccga gcgcgaccag cccggcaacg
     1801 atgccggctc gctgccacag gttg
//
LOCUS       DASUCJ010000160         5780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1132529, whole genome shotgun sequence.
ACCESSION   DASUCJ010000160 DASUCJ010000000
VERSION     DASUCJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132529"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..625)
                     /locus_tag="VFU90_01995"
     CDS             complement(182..625)
                     /locus_tag="VFU90_01995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEU5478569.1"
                     /translation="MRLPLEDAPAEATLFADGGSRGNPGPAASGAVLIDPDGVVIEER
                     GLYLGVATNNLAEWNALLIGLEAAAARGVRHLAIRLDSELVVKQLRGEYRVKHAALQP
                     LHRRAQALMRRFERIDLRHVPRKQNALADRLVNRVLDQEAQDKAK"
     gene            687..1034
                     /locus_tag="VFU90_02000"
     CDS             687..1034
                     /locus_tag="VFU90_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478570.1"
                     /translation="MKRFIPTALAAATFAAAVAAVSSTALAASTFYGIVVHVSTSNIK
                     VQDPKSKQTLSFTIVPKFDQVFSANGKTTYQMSAIKAGQYVGIIYDQKALGIRHAVKI
                     YLLTNANQRIGTQ"
     gene            1087..1449
                     /locus_tag="VFU90_02005"
     CDS             1087..1449
                     /locus_tag="VFU90_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478571.1"
                     /translation="MKLLIRSAIAALALGTLLGSLSAALPASAQYSPYPTYTTWQRDW
                     DDYHYDRQHVMLGVVTDFSPYRLTVRRRNGVDQLVDLRNGTVIYPTGATPTPGERVAL
                     VGRYSNGVFVVDRVIIRG"
     gene            1592..2908
                     /locus_tag="VFU90_02010"
     CDS             1592..2908
                     /locus_tag="VFU90_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5478572.1"
                     /translation="MRNITIRTAAAFVCIANLPLLCRADDAQMFRVDAAHRGVYDSAP
                     SPLRAVKWRFRTGGMIFASPSVADGVVYVGSADGKLYAVRAADGALVWKFATGGPVNS
                     SPAVVNGVVYFASLDGNVYAVGAADGRRRWRYETEGERRFTAPGIHGMIPRTQLMPDP
                     FDVFLSSPAVARGTVYIGSGDHFVYALDAASGALRWRVRTGNVVHASPAVSNGTVYIG
                     SWDRYFYALDARTGAVRWKFQTGDDRNIYNQVGIAGSAAVDGGTVYFGCRDSFFYALD
                     ARTGALRWKQDQHGSWVIASPAISNGSVYFTTSDEKVFWALDAATGAPRFKVPYGAFS
                     FSSPALAGDVAYFGTFDGRLYGVDVRSGTIVARYATDGSVRNLPSHLDARGSVDLNGF
                     YPDETYEGIVVGLNRIYSLGSIPGSPALAGGVLFVGSTDGTLYAVD"
     gene            complement(2905..3402)
                     /locus_tag="VFU90_02015"
     CDS             complement(2905..3402)
                     /locus_tag="VFU90_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5478573.1"
                     /translation="MPERRLALAGGQTPYATLLTCSDSRVAPELVFDEHVGDLFVFRN
                     AGNFVVPEVLGTLEYGYSALGVKLIVVMGHTSCGAVNATYDAVAEHKALPPNLDLIQK
                     AIQDGIRPVVVAHGSKDAATVANAKAQTAKLKTSPVLGPAIASGDCKIVAAEYHLASG
                     KVTFY"
     gene            3765..4256
                     /locus_tag="VFU90_02020"
     CDS             3765..4256
                     /locus_tag="VFU90_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478574.1"
                     /translation="MNTTTDFESGLYDDPAQAETRIQELHRLGYTDREISVMMRDRER
                     ARAFVERAGGHATEGAVTGGVIGGGIGAIIAGLMATGSIVAIAGTGGVAAPLVAGPLA
                     AVLAGLGAGGTAGGIIGALIGAGIPKERAEAYSAGLDRGGILIGVSPRPGDREAVRRI
                     FED"
     gene            4609..5226
                     /locus_tag="VFU90_02025"
     CDS             4609..5226
                     /locus_tag="VFU90_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU5478575.1"
                     /translation="MYIPPSFEIADPVRALEIIASHPFGLLITCEEGIPYVSHLPFIA
                     ERRAEGLSVIGHVARGNPHARAVSAERPATVVFNGPHAYVSASWYERPYETVPTWNYA
                     AVHLCGRLQETDAWQAVRALSDAMEGPGAGAWDPQRLDPAYRDGQLRGIVAFRLEATA
                     VYAKAKLSQNRSDADRLRVMRRLLASDDQTARECGEMMRRSNESA"
     gene            complement(5218..>5780)
                     /locus_tag="VFU90_02030"
     CDS             complement(5218..>5780)
                     /locus_tag="VFU90_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5478576.1"
                     /translation="SNPLSEEGCLGGAHHPGNAESSANRSSGADSAVRVPAAMFGELA
                     PHAIEAVLHEELFARIGYVDADGLPAIAPISYAYDGRSIYGYSLFGTKIAEMSAHPKV
                     CVEIDRVDDAATWRSVVLRGTFEPLEGLEAMEAVERISERLRTVAAATGAPPAAWRTY
                     VMRAGGDGIAYRIRITEKHGRYSALS"
BASE COUNT          987 a         1884 c         1818 g         1091 t
ORIGIN      
        1 cggcttgacg taccggatgg tcagtcgcgc gtacagttgg ctcggcgcgt tcttggcgtg
       61 actgaggcct gaaacgggat gcaaacctcc gtagacgcgc caaccgacta ccaaaaagat
      121 cgccgtcagg acgacgatga aagcttcgag cggcgggaag cggcgcatgc tgagtgcggc
      181 tctactttgc cttgtcctgt gcttcctggt cgagaacgcg gttcaccaag cggtcggcga
      241 gcgcgttctg ttttcgcggc acgtgacgga ggtcgatgcg ttcgaatcgc ctcattaagg
      301 cctgggcgcg ccggtgcagc ggctgcaatg cggcatgttt gacgcggtat tcgccgcgca
      361 gctgcttgac cacgagttcc gaatccaagc gaatcgccag atgccgaacg cctcgcgccg
      421 cggcggcctc caaaccgatc aataaggcgt tccattcggc taggttgttg gtggcgacgc
      481 cgaggtagag accgcgctcc tcgatcacga cgccgtcggg atcgatcaaa acggccccgc
      541 ttgccgccgg tccgggattt ccgcgggagc ctccgtcggc aaagagggtc gcttcggcag
      601 gggcgtcctc gagcggcagt cgcatcggat ggctgttcgg cggccccgcg gcatgaaccg
      661 cccgcccggc gggtaaaggc atccgcatga aacgctttat tccaaccgcc ctcgcggcgg
      721 caaccttcgc agccgccgtc gcggccgtat cgagtacggc actggccgct tcgacctttt
      781 acggcatcgt cgtgcacgta tcgaccagca acatcaaggt tcaggatccc aagtcgaaac
      841 agacgttgag cttcacgatc gtcccgaagt tcgaccaggt tttctcggcc aacggcaaga
      901 cgacgtatca gatgagcgcg atcaaagccg gccagtacgt cgggatcatt tacgatcaga
      961 aggcgctcgg cattcggcac gccgtcaaga tctatttgct gactaacgcc aaccagcgga
     1021 tcggtacgca gtaaccggtc gcttcgcgcg tttcgcgtcg cggcgcgttt gggtaaccgg
     1081 ttccttatga aacttctcat acgttccgcc atcgccgcgc tggcgctcgg caccctgctc
     1141 ggctcgttgt cggccgcgct gccggcgtcg gcgcaatact cgccgtatcc gacgtacacg
     1201 acgtggcaac gcgattggga tgactaccat tacgaccgcc agcacgtcat gctcggggtc
     1261 gtcacggact tttcccccta tcgccttacg gtccggcgcc gtaacggcgt cgatcaactc
     1321 gtggatctgc gtaacggcac cgtgatttat ccgaccggtg ccacgccgac cccgggagaa
     1381 cgcgtcgctc tcgtcggccg ctacagcaac ggcgtgttcg tcgtcgaccg ggtgatcatt
     1441 cgcggttagg caccgcaccg gcgccgccgg cggctctcac gccgccggcg agccgggatc
     1501 gtccggcggg aaccacgcaa ttgatggacg tttgagcgtt ttgacacacc actgaaccgg
     1561 agttgttgcc gcacgcacga acggcgattg catgcgcaac atcacgatcc gcaccgccgc
     1621 cgcgttcgtt tgcatcgcga atcttccgct gctttgcaga gccgacgacg cccagatgtt
     1681 ccgcgtcgat gcggcgcatc gcggggtcta cgactcggct ccttccccgc tccgcgccgt
     1741 caaatggcgc ttccgtaccg gcgggatgat cttcgcttcg ccgagcgtgg cggacggcgt
     1801 cgtctacgtc ggaagcgccg acggcaagct ctacgccgtt cgcgccgccg acggcgcgct
     1861 cgtctggaag ttcgccaccg gaggacccgt caattcgtcg ccggcggtcg taaacggcgt
     1921 cgtctacttc gcgagcctcg acggcaacgt ctacgcggtc ggcgcggccg acgggcgccg
     1981 gcgctggcgt tacgagaccg aaggcgagcg gcgctttacg gcgccgggta tccacggaat
     2041 gattccgcgt acccagttga tgcccgatcc attcgacgtg tttctctctt cgccggccgt
     2101 agctcgcgga accgtgtaca tcggcagcgg cgaccacttc gtgtacgcgc tcgatgcggc
     2161 tagcggcgcg ttgcgctggc gcgtccgaac cggcaacgtc gtgcacgcgt ctcccgccgt
     2221 ttctaacggc accgtctaca tcggcagctg ggaccgctac ttctacgcac tcgacgcgcg
     2281 taccggagcc gttcgctgga aattccagac cggcgacgat cggaacattt acaaccaggt
     2341 tggaatcgcc ggttccgcag ccgtcgacgg cggcacggtc tacttcggct gccgggacag
     2401 cttcttctat gcgctcgacg cccgaaccgg agcgctgcgc tggaaacagg atcagcacgg
     2461 gagctgggtc atcgcttcgc cggcgatttc gaacgggtcg gtctacttta cgacgtcgga
     2521 cgagaaggtt ttttgggcgc tcgatgcggc gacgggcgca ccgcgattca aggttccgta
     2581 cggagccttc tccttctcct cgccggcgct tgccggtgac gtggcctact ttgggacgtt
     2641 cgatggccgg ctttacggcg tcgacgttcg cagcggaacg atcgtggcgc gctatgcgac
     2701 ggatggctcc gtacggaacc ttccgtcgca tttggacgct cgcgggagcg tcgacctcaa
     2761 cggattttac ccggacgaga cctacgaggg aatcgtcgtc ggcttgaacc ggatttattc
     2821 ccttgggtcg attcccggct cgccggcact cgccggcggc gttctgttcg tcggcagcac
     2881 cgacggtacg ctctatgccg tcgactagta gaacgtcacc ttcccgctcg cgagatgata
     2941 ctcggccgcg acgatcttac aatcgccgct ggcaatcgcc ggtccgagaa cgggcgacgt
     3001 cttcaacttc gcggtttgtg ccttggcgtt ggcaacggtg gcggcgtcct tgctgccgtg
     3061 cgcaacgacg accggccgaa ttccgtcttg aatcgccttc tggatgaggt cgagattcgg
     3121 cggcagcgcc ttgtgctcgg ccacggcgtc gtaggttgcg ttgaccgccc cgcaactcgt
     3181 atgccccatc acgacgatca gttttacgcc gagcgcgctg tagccgtatt cgagtgtacc
     3241 gagaacctcg ggaacgacga agttccccgc gttgcgaaac acgaacaaat cgccgacgtg
     3301 ttcgtcgaat accagctcgg gagcaacgcg cgagtcggag caggtgagca gcgtcgcgta
     3361 cggcgtctga ccgccggcca gcgcgaggcg gcgctcggga atgccttcgt agttctaaat
     3421 cgtgccggac acgaaccgcg cgttaccagc gattagtcgc gcgagcgcgt cgcttggcgt
     3481 gactccggct cccgccgagg ccgccgtggc gggaagatct gccgaaacca cgcccaacga
     3541 cgcgaatgcg gcggcgccga ggcgaatgac gtctgctctg gagagagaat cgctcacgaa
     3601 tttctcctat ctcgttcggt tgaaggaaac tccgaaaaca gttcgcggcg gcaacgcttt
     3661 ctactgcagc gaagagtcac ccgctcgttc gctacgatac gctcgaggac gcgtccggta
     3721 cggcaacgcg tttacggaaa accgtttcgg gtaacaccaa cctcatgaac actacgaccg
     3781 atttcgaatc cggactctac gacgaccccg cgcaagccga aacgcgcatt caagaacttc
     3841 atcgcctggg ctacacggac cgcgagatca gcgtgatgat gcgcgatcgc gaacgcgcac
     3901 gtgcgtttgt ggagcgcgcc ggcggccatg ccaccgaagg agccgtgacc ggcggcgtaa
     3961 tcggcggcgg catcggtgcc atcatcgccg gactcatggc gacgggcagc atcgtcgcga
     4021 tcgccggaac cggcggcgtg gcggcaccgt tggtagcggg gccgttggcc gccgtactcg
     4081 ccgggcttgg cgccggcggc acggccggcg gcatcatcgg tgccttgatc ggcgccggca
     4141 ttcccaaaga gcgagccgaa gcctactccg ccggtctcga ccgcggggga attttgatcg
     4201 gcgtgagtcc gcgccccggc gaccgcgaag ccgtcagacg catcttcgag gattgagtct
     4261 tttaccacca cccgattgca ccggtgcgga cgcgctttgt gcgcttccgg cgccggtgcg
     4321 cgggcatgac gtactttttc gtgcgatcgc cggcgctagt tcgccttgca atgtaaatcg
     4381 gaggtggtca ctcagcacgg cctcggcctc gggctaagtg accaacgcct tcttacccac
     4441 caacgcggcg actgatgccg gacgctcggc gttctaacct ctctctaggg aaccttcgaa
     4501 gaactcgtgc aagtcgtgca cggttggcat tccaggaccg ccctacgcgc ttacggaacg
     4561 acgcgttgcc gggtcgcttc cgttgaagaa aggacgtagc gcgatctcgt gtatatcccg
     4621 ccgtcttttg agatcgccga tccggtgcga gccctcgaaa tcatcgcatc gcacccgttc
     4681 ggcttgctga ttacgtgcga ggaggggatt ccgtacgtct cccacctgcc gttcatcgcg
     4741 gaacggcgag cagaaggtct ttcggtcatc ggacacgtgg ctcgcgggaa tccccacgca
     4801 cgtgcggttt ccgccgaacg cccggcaacc gtcgttttca acggaccgca tgcctacgtt
     4861 tccgcgtcgt ggtacgagcg gccgtacgaa accgtgccga cgtggaacta tgccgccgta
     4921 cacctctgcg gccgtttgca ggagaccgac gcttggcagg ccgtgcgcgc gctcagcgat
     4981 gcgatggagg gtcccggagc cggcgcttgg gatccgcagc gactcgatcc cgcttaccgc
     5041 gacggtcaac tgcgaggcat cgtggcgttc cgcctcgagg ccaccgccgt gtatgccaaa
     5101 gcgaagctga gccagaatcg cagtgacgcc gaccgtttac gcgtcatgcg ccggctcttg
     5161 gcttccgacg atcagaccgc gcgcgaatgc ggtgaaatga tgcgccgttc taacgagtca
     5221 gcttaacgcc gagtagcggc cgtgcttctc cgttatgcga atgcggtagg caattccgtc
     5281 gcctccggct cgcatgacgt acgtgcgcca ggccgccggc ggagcgccgg ttgcggcggc
     5341 gacggttcgc agccgctcgg aaatgcgctc gacggcttcc atcgcctcga gaccttcgag
     5401 tggttcgaac gttcctcgaa ggacgaccga tcgccacgtg gccgcgtcgt ctacgcgatc
     5461 gatctcgacg caaaccttcg gatgcgcgga catctccgca atcttcgtcc cgaacaggct
     5521 ataaccgtaa atcgaccttc cgtcgtatgc gtagctgatc ggggcgattg cggggaggcc
     5581 gtcggcatcg acgtacccga tccgcgcgaa gagctcttcg tgcagcaccg cctcgatggc
     5641 gtgcggcgct agctcgccga acatggccgc cgggacgcgc actgctgaat ccgcaccgga
     5701 cgaccggttg gcgctcgatt cggcgtttcc cgggtggtgt gcgccgccca gacacccctc
     5761 ctccgatagc ggatttgagc
//
LOCUS       DASUCJ010000161         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1025670, whole genome shotgun sequence.
ACCESSION   DASUCJ010000161 DASUCJ010000000
VERSION     DASUCJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025670"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFU90_02035"
     CDS             complement(<1..510)
                     /locus_tag="VFU90_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478577.1"
                     /translation="MTVPLRPTTRLQRPRTRARVTSVFVAGLACAIATACHRPKPNPP
                     TADFLLFTADSTFWIHSGPQGIHARGSPIQLARYGGKYYEVYVADDDRSYTDAEIVGQ
                     QVWRRDILSGDSSLVFRDTTIEGIEHWYARTHPDDHPLDPNDDSDDDDPRVSATSELD
                     LLDQFGPYVS"
     gene            626..1774
                     /locus_tag="VFU90_02040"
     CDS             626..1774
                     /locus_tag="VFU90_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5478578.1"
                     /translation="MRKTLWLIAVSAACGLAVLCPTLSAQAGPAAAARPVSLDDTTIV
                     RALYVNRWASQSPRRMRQLIALADSTEINALVIDMKDEFGINYESTDPVVERNAGHGG
                     RIPHLAELLDTLRAHRIRPIARLVVFKDSVAARVNPDHVIHQPNGQPWRDKKGLTWVD
                     PYDTTIWDYDIRVAEEMAQLGFAEVQFDYIRFPEPYPSLPPQVFRDANGLTKPQALAR
                     FLEAACPRVRKAGALCTADIFGLVTTVPGALEVGQQWEPLSPRLDVLLPMVYPSLYPH
                     GAFGLALPNANPYQVIYDATSRAHERDLKLQIASSAHVRPWLQAFTLGKPRYGAHELE
                     EEKRAVYDAGYDSWTLWNPGSLYEAFVPALEKTTVSRKKPFGNKEASR"
     gene            complement(1791..3041)
                     /locus_tag="VFU90_02045"
     CDS             complement(1791..3041)
                     /locus_tag="VFU90_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEU5478579.1"
                     /translation="MTSAPITADGRVQSTDAIRQHFPALERRQAGRWVAYFDGPGGTQ
                     VPRMVTDAVTAYLLHHNANTHWAYPTSIETDAAIARARRAIADLLNARDDEIAFGQNM
                     TSLTFHVARGIGRRLSVGDEIVVTELDHHANIAPWRALEAERGVTVRMVRMHPDTGQL
                     DWDDLERSIGRRTRVVAIGAASNALGTINDVARATRLAHAAGAIAFVDAVHYAPHQLV
                     DVRAIGCDFLACSAYKFYGPHVGILYGTSERILEVDVPRLEPAPDTAPERLETGTQNH
                     EGIVGAAAAVDFLASLGAGDTRRARLASAFEALHARGTALVTSMWNGLSAIPGVRVFG
                     PPPSSPRTPTISFAVAGRSSTDVARELADRAVYASNGDFYAATVIERIGMAPDGVVRA
                     GCACYTTQDEVDRLVAAVTEIARR"
     gene            complement(3038..3928)
                     /locus_tag="VFU90_02050"
     CDS             complement(3038..3928)
                     /locus_tag="VFU90_02050"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5478580.1"
                     /translation="MTQIAFLGLGAIGTPMARHLAQPPFTLTVWNRTRARADEFARST
                     GARAAATPRDATTGAAFVVTCLPTSREVESLLDGADGLIAGMARGSMLIDCTSGDPAT
                     SRRIAARLGERGIGFVDAPVSGGTVGAERGTLTVMCGGDAESFARARPIIDAFGEKLV
                     HCGPVGAGHALKSVNNALLAVHVWATGEGLAALAKAGVSPTIALDVINASSGRSNTSQ
                     NLFPERVLTRAFPRTFRLALLDKDLGIAAGVAREHHVASPLLQLTSELFRAAHTLLGE
                     EADHVEAVRVIEAWSGVEIT"
     gene            4218..4457
                     /locus_tag="VFU90_02055"
     CDS             4218..4457
                     /locus_tag="VFU90_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU5478581.1"
                     /translation="MRITTSVTIQGMLSVHAAHAVFTALTGVEGISTADVKVGRATIE
                     HDGRATHEQIIDAIETAGYVVTDIRDERRLPLLDR"
     gene            complement(4454..>4773)
                     /locus_tag="VFU90_02060"
     CDS             complement(4454..>4773)
                     /locus_tag="VFU90_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478582.1"
                     /translation="RQMVLFRAVDIGAELFAMSASCVRAVNVARNGDANAIDLADVFC
                     REARLRIAEHHRNLFGPTDGALYRLSQRVMQGEFTWLEQGIVAMESEETETATAELIA
                     AAR"
BASE COUNT          748 a         1539 c         1703 g          783 t
ORIGIN      
        1 cgacacgtac ggtccgaact ggtcgagcag atcgagctcg ctggtcgctg agacgcgcgg
       61 atcgtcgtcg tccgaatcgt cgttgggatc gagcgggtgg tcgtcgggat gggtgcgtgc
      121 gtaccagtgc tcgatgcctt cgatcgtcgt gtcgcggaac acgagcgacg agtcgccgct
      181 cagaatgtct cgtcgccaca cctgctggcc gacgatttca gcgtcggtgt aggagcggtc
      241 atcgtcggcg acgtacacct cgtaatactt gccgccgtaa cgtgccaact ggatgggcga
      301 tccgcgtgcg tggatccctt gcggtcccga gtggatccag aatgtcgagt cggcggtgaa
      361 gaggagaaag tcggccgtcg gcgggtttgg tttcgggcga tgacacgcag tcgcgatcgc
      421 gcacgccagt ccggcaacga aaacggaggt gacgcgggcg cgggtgcgcg gccgttgcaa
      481 gcgggtggtt ggacggagcg gtacggtcat gcggcgtgca tggaaatata gcgtgtgacg
      541 ggagcgggcc gcgcgtcgta cattgtgcag atgcggcggg caaggccgct actctccatt
      601 cggtcgtgcg gagcaccgat catccatgcg aaaaactttg tggctcatcg ccgtgtccgc
      661 cgcgtgcggg cttgcggttc tctgcccaac gctctccgcg caggcggggc cggcggccgc
      721 ggcgcgtccg gtgtcgctcg acgacacgac gatcgtgcgc gcgttgtacg tgaatcgttg
      781 ggcgtcgcag agtccgcgcc ggatgcggca gctcatcgcg ctggccgatt cgacggagat
      841 caacgcgctc gtgatcgaca tgaaggatga attcggcatc aattacgagt cgacggatcc
      901 ggtcgtggag cggaacgccg gacacggggg ccgcattccc catctggccg agctgttgga
      961 cacgctcagg gcgcaccgca tccggccgat cgcgcggctg gtggtgttca aggattcggt
     1021 ggcggcgcgc gtgaatcccg accacgtgat ccaccagcct aacggacagc cctggcgcga
     1081 caagaagggc ctgacgtggg tggatccgta cgacacgacg atctgggact acgacatccg
     1141 ggtcgccgag gagatggccc agttaggctt cgccgaggtg cagttcgatt acatcaggtt
     1201 cccggagccg tatccgagtc tgccgccgca ggtgttccgc gatgcgaacg ggttgacgaa
     1261 gccgcaggcc ctggcgcgtt ttctcgaggc ggcgtgtccg cgcgtccgga aagcgggcgc
     1321 gctctgcacc gccgacatct tcgggctggt gacgacggtt cccggtgcgc tcgaggtggg
     1381 ccagcagtgg gagccgttgt cgccgcggct ggacgtgctg ctgccgatgg tgtacccgtc
     1441 gctctatccg cacggcgcgt tcgggctggc gctgcctaac gccaacccgt accaggtgat
     1501 ctacgacgcg acgagccgcg cccacgagcg cgacctcaag ctccagatcg cctcgtcggc
     1561 gcacgtgcgc ccctggctgc aggcgttcac gttgggcaag ccgcgctacg gggcgcacga
     1621 gctggaggaa gagaagcggg cggtgtacga cgccggctac gacagttgga cgttgtggaa
     1681 tccgggctcg ttgtacgaag cgttcgtgcc ggcgctcgag aagacgacgg tctcgcgcaa
     1741 gaaaccgttc ggaaacaagg aagcgtcgcg gtaggtacgc ggcgctgccg tcaccggcgc
     1801 gcgatctcgg tcacggccgc gacgagccgg tcgacctcgt cctgcgtcgt gtagcacgcg
     1861 cagccggcgc gcacgacgcc gtcgggcgcc atgccgatgc gctcgatcac cgtggccgcg
     1921 tagaagtcgc cgttggatgc gtagactgcg cgatcggcga gctcgcgcgc gacgtcggtg
     1981 gatgagcggc cggcgaccgc aaacgagatc gtgggcgtgc gcggcgacga tggcggcggc
     2041 ccgaacacgc gcacgccggg aatcgcggac agcccgttcc acatgctcgt gactaacgcg
     2101 gtgccgcgtg cgtgcagcgc ctcgaatgcc gacgcgagcc gcgcgcgccg cgtgtcgccg
     2161 gcgccgaggg atgcgaggaa gtcgacggcc gccgcggcgc cgacgatgcc ttcgtgattc
     2221 tgcgtgcccg tctcgagccg ctcgggcgcc gtgtcgggcg cgggctcgag ccgcggcaca
     2281 tccacctcca gaatgcgctc gctggttccg tacaggatcc ccacgtgcgg cccgtaaaat
     2341 ttgtacgccg agcacgcgag gaagtcgcag ccgatggcgc gcacatcgac gagctggtgc
     2401 ggcgcgtaat gcacggcgtc gacgaacgcg atcgcccccg ccgcgtgcgc gagccgcgtg
     2461 gcgcgcgcca cgtcgttgat cgtgcctaac gcattggacg cggccccgat cgccaccacg
     2521 cgcgtgcgcc tgccgatcga ccgctcgaga tcgtcccagt ccagctgtcc ggtgtcggga
     2581 tgcatgcgca ccatgcgcac ggtcacgccg cgctccgcct cgagcgcgcg ccagggcgcg
     2641 atgttggcgt ggtgatcgag ctcggtcacc acgatctcgt cgccgacgct cagccggcgc
     2701 ccgatgccgc gcgccacgtg aaacgtgagc gacgtcatgt tctgcccgaa cgcgatctcg
     2761 tcgtcgcgcg cattcagcag gtcggcgatg gcccgtcgcg cacgcgcgat cgccgcgtcc
     2821 gtctcgatgc tcgtgggata cgcccagtgc gtattggcgt tgtgatgcag caggtacgcg
     2881 gtcacggcgt cggtgaccat acgcggcacc tgcgtgccgc cgggtccatc gaagtatgcg
     2941 acccagcggc cggcctggcg ccgctccaag gccgggaagt gttggcgaat cgcgtccgtg
     3001 gattggacgc gtccgtcggc ggtgatgggg gcgctggtca cgtgatctcg acccccgacc
     3061 acgcttcgat gacgcgcacg gcctcgacgt gatcggcctc ttcgcctaac agggtgtgcg
     3121 cggcgcggaa cagctcgctc gtgagctgca agagcggcga tgccacgtgg tgctcgcgcg
     3181 cgacgccggc ggcgatgccg aggtccttgt cgagcagcgc caggcgaaag gtgcgcggga
     3241 atgcgcgcgt gagcacgcgc tcgggaaaca ggttctgcga tgtgttgctg cgtccgctcg
     3301 atgcgttgat cacgtccagc gcgatcgtgg gcgacacgcc cgctttggcg agcgcggcga
     3361 gtccttcgcc ggtagcccac acgtgcacgg ccagcagcgc gttgttcacg gatttgagcg
     3421 cgtggccggc gccgaccggg ccgcagtgga cgagcttttc gccgaacgca tcgatgatgg
     3481 gccgcgcgcg agcgaacgat tcggcgtctc cgccgcacat caccgtcagc gtgccgcgct
     3541 cggcgccgac ggtgccgccg ctgaccggcg cgtcgacgaa cccgatgccg cgctcgccta
     3601 accgtgccgc gatgcggcgc gacgtcgccg gatcgcccga cgtgcagtcg atgagcatgc
     3661 tgccgcgcgc catgccggcg atcaggccgt cggcgccgtc gagcagcgac tcgacctcgc
     3721 gggacgtcgg caggcacgtg accacgaagg cggcgcccgt cgtcgcgtct cgcggcgtgg
     3781 cggcggcacg ggcgccggtg ctgcgtgcga attcgtccgc gcgtgcacgc gtgcggttcc
     3841 ataccgtgag tgtgaacggc ggttgggcga ggtggcgcgc catcggcgtg ccgatcgcgc
     3901 ccaatccgag gaatgcgatc tgagtcatgc gacgaagtgg tggcgaggac accggaactt
     3961 cgttgacagg cggcgcgatg tcaaaggcgt agagcggatt gaacggagaa cgccacggat
     4021 aaaggcacgg atcgctcggc tgctcggcgc cgggtaacct ggtcgtacgt tggtgctgtt
     4081 tgtttttcaa caaacaacgg atcgatgcct accacgcgtg atagaggcgg cgccgagccg
     4141 tcgagcgatc cgtgcgttct ccgtgcagtc atccgttcaa tccatgtctc cagagcgtgg
     4201 aagcgctacc ttgcggcgtg cgcatcacga cgtcggtcac catccagggc atgctgtccg
     4261 tacatgcggc gcacgccgtc ttcaccgccc tgacgggggt ggagggcatc agtacggcgg
     4321 acgtgaaagt gggtcgcgcg accatcgagc atgacggtcg ggcgacccac gagcagatca
     4381 tcgacgcgat cgaaacggcc ggctacgtcg tgaccgacat ccgcgacgag cggcggctgc
     4441 cgcttctcga ccgctagcgc gcggccgcga tcagctcagc agtggccgtt tcggtctctt
     4501 ccgactccat ggcgacgata ccttgttcga gccatgtgaa ctcgccctgc atcacgcgtt
     4561 gcgacagccg gtagagcgcg ccgtccgttg ggccaaacaa attgcggtgg tgctcggcga
     4621 tgcgcagccg ggcttcgcga cagaacacgt cggcgaggtc gatggcgttt gcgtcgccgt
     4681 tcctggccac gttcaccgcg cgcacgcacg atgccgacat cgcgaacagc tcggctccga
     4741 tgtcgacggc gcggaagagc accatctgcc gtt
//
LOCUS       DASUCJ010000162         1490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_139347, whole genome shotgun sequence.
ACCESSION   DASUCJ010000162 DASUCJ010000000
VERSION     DASUCJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139347"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU90_02065"
     CDS             <1..184
                     /locus_tag="VFU90_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478583.1"
                     /translation="RCRLNPGARPIAPLEGVRMSRERMYADTSRARRELGHQPTSVRA
                     ALERAVAWYRANGYGE"
     gene            358..1341
                     /locus_tag="VFU90_02070"
     CDS             358..1341
                     /locus_tag="VFU90_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478584.1"
                     /translation="MLTAVALGSLALPSCSGGSSLPNSGVSAPASHTQSSARRLPQTL
                     GENAQAAALYVSDGTAKSIFRFARNPGGTLVAPPGSSLVLPYNPGPIAIGRHGNLFVT
                     DQGNESIEVYAKGATGYQQPSRTLLLPFAPSSVAVDANGYEYVGGYSNGFVAVYKPGA
                     HGMAGTIQRIALPDGHPTINGVAVDPSGNLYVSDSNEVSEFGTPTTNPTLLRAIIGSG
                     ELNQPTGMAVAPKSGELYVANAGDGNILAYSPTANGKSHPDRTISSSNPSLVLPVAIA
                     LGGSALYSTSGSKLYGTPSVFLLDAQRGHQTPKQVVTGNYLAAPVGVALGP"
BASE COUNT          265 a          496 c          488 g          241 t
ORIGIN      
        1 gcgctgccgg ttgaatcccg gcgctcgccc gattgccccg ttggaaggcg tgcgcatgtc
       61 gcgcgagcgg atgtatgcgg atacgtcgag ggcccggcgc gaactcgggc accagccaac
      121 gtcggtccgc gccgcgctcg aacgtgcggt ggcctggtat cgcgctaacg gctacggcga
      181 gtagcagtcg ccgcagccgg ggatcggcgc gcctcggctc cgccttcgta cgaccaacgc
      241 acggataggc aaccgagcgt cggcctccaa tgatgggcgc tcgtcaaccg ggtaagaacc
      301 ggtgacctgc tcccgacctt ggaggcgaag atgtttcgat cacggctaac gatacgggtg
      361 ctgacggccg tcgcactcgg aagtctcgcg ttacccagtt gtagcggagg gagttcgctc
      421 cccaattccg gcgtttcggc gccggcgagt catacgcaat cctccgcccg gcgattaccg
      481 caaactctcg gcgaaaacgc tcaggcggcc gcgctgtacg ttagcgacgg aaccgcgaaa
      541 tcgatcttcc ggttcgcgcg caatccgggt ggtacgctcg tagcgccccc cggttcgtcg
      601 ctggtgctgc cgtacaatcc cgggccaatc gcgatcgggc ggcacgggaa tctattcgtg
      661 accgaccagg gcaacgaatc gatcgaagtg tacgcgaagg gtgcgacggg ttatcagcaa
      721 ccgagcagaa cgctgctcct gccgttcgct ccaagcagcg tcgccgtcga cgctaacggc
      781 tacgaatacg tcggcggcta tagcaacggg ttcgtcgccg tgtataagcc tggagctcac
      841 ggaatggccg gcacgatcca gcgcatcgcg ctccccgacg ggcatcctac gatcaacggc
      901 gtcgccgtcg atccttcggg taacctgtac gtttccgatt cgaacgaagt cagcgagttc
      961 ggcacgccga ccaccaaccc gacgctgctg cgcgcgatca tcggttcggg cgaactcaat
     1021 cagccgaccg ggatggccgt tgcgccgaag agcggcgagc tgtacgtcgc caatgccggg
     1081 gatgggaaca tcctggcgta ctcgccgacg gccaacggca agagccaccc ggaccgaacg
     1141 atctcgtcga gcaacccgtc gctggttctg ccggtggcga tcgcgctggg aggcagcgcc
     1201 ctctactcga ccagcggttc gaaactgtac ggcactccct cggtgttcct gctcgacgcg
     1261 cagcgcggcc accagacgcc caagcaggtc gtcaccggca actaccttgc ggcgccggtc
     1321 ggcgtcgcgc tgggtcccta acgtatgatg tgacccggcg ggccgaggag cggcccgccg
     1381 gttagccaag ccgcgcagga atgtacgccg aggtggccgt cgttgcagcg accggcttgg
     1441 aagcgaagga agcgcgccga gccctacccg gcatccgcgt ttacgagcgg
//
LOCUS       DASUCJ010000163         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1331252, whole genome shotgun sequence.
ACCESSION   DASUCJ010000163 DASUCJ010000000
VERSION     DASUCJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331252"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFU90_02075"
     CDS             complement(<1..983)
                     /locus_tag="VFU90_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478585.1"
                     /translation="MERRVLLENPAAMQEASALMRRAAGGVHAEAVPAVGDTLTFNVP
                     NANATNACQTFTTQRAVVKTVGTHGIIVQDITAPAGGFTAAAFDSIAAEFDQIIYPAD
                     TVHFGQPSDIDSNGHVFLFFTPQVNAATKRGSNSILEGFFFVGDLFATTSCTESNQSE
                     LFYLLTPDPTGKFSDSRSTSSVRQDIRGTIAHEFQHMINAGVRLSANAPDEATWLNEG
                     LSHFAEELVGRAEDNFTDTQKLTIGDVADFPSLNNFDAFFGQNMARLREFMLKPGALG
                     ATSGHADTSLAVRGAAWSLLRYSADHYANGTVAAFTRALVVGPDTGVANLVA"
BASE COUNT          170 a          353 c          315 g          180 t
ORIGIN      
        1 gccaccagat tcgccacgcc cgtgtccggc ccaaccacga gcgcccgcgt gaacgcggcg
       61 acggtcccgt tcgcgtagtg gtccgccgaa tagcgaagca gcgaccacgc cgcgccgcgc
      121 accgcgagcg acgtgtccgc atgcccgctc gtcgcgccta acgcaccggg cttcagcatg
      181 aactcccgca ggcgcgccat gttctgtccg aagaacgcgt cgaagttgtt cagactcggg
      241 aagtcggcca cgtcgccgat cgtcagcttc tgcgtatccg tgaagttgtc ctcggcgcgg
      301 ccaaccagct cctccgcgaa gtgcgacaga ccctcgttca accacgtggc ctcatccggg
      361 gcgttcgcgc tcaggcgcac accggcgttg atcatgtgct gaaactcgtg cgcgatcgtg
      421 ccgcgaatgt cctggcgcac cgacgacgtc gagcgcgagt cgctgaactt ccccgtcgga
      481 tcgggcgtga gcagatagaa cagctccgat tggttgctct cggtgcagct ggtggtcgca
      541 aacaggtcgc cgacgaagaa aaagccctcg agaatgctgt tcgatccgcg cttcgtcgcc
      601 gcgttcacct gcggcgtgaa aaacaggaat acgtgcccgt tgctgtcgat gtccgacggc
      661 tgcccgaagt gcaccgtgtc ggcgggataa atgatctgat cgaattccgc cgcgatggaa
      721 tcgaatgccg cggcggtgaa tccgccggcc ggcgccgtga tgtcctgcac gatgatgccg
      781 tgcgtgccca ccgtcttcac caccgcgcgc tgcgtcgtga acgtctggca cgcgttcgtc
      841 gcgtttgcgt taggcacgtt gaatgtcagc gtatcgccga cggccggcac cgcctccgcg
      901 tgaacaccgc cggcggcgcg tcgcatgagc gccgacgcct cctgcatcgc cgcgggattc
      961 tccagcagca cccgccgctc catcgcgcgg atccgtcctt ccgcgatcga gttatacg
//
LOCUS       DASUCJ010000164         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1499413, whole genome shotgun sequence.
ACCESSION   DASUCJ010000164 DASUCJ010000000
VERSION     DASUCJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499413"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFU90_02080"
     CDS             <1..216
                     /locus_tag="VFU90_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478586.1"
                     /translation="GLGYAEALAWSRGEMTRREAIASTTQRTIRYAKRQRTWFRRMDP
                     ALRVDAADARAATEAIIRMARESIARA"
     gene            294..479
                     /gene="hfq"
                     /locus_tag="VFU90_02085"
                     /pseudo
     CDS             294..479
                     /gene="hfq"
                     /locus_tag="VFU90_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
     gene            666..>1096
                     /gene="dapF"
                     /locus_tag="VFU90_02090"
     CDS             666..>1096
                     /gene="dapF"
                     /locus_tag="VFU90_02090"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5478587.1"
                     /translation="MLLDRTAGSELAYEDIAQTICDRHLGVGADGLLVLERAKSPGAD
                     VALRIFNADGSAADMCGNGIRCVARYMHERTPSAPRRLRIETPSGDVRTEMVSDSPFS
                     VRVDMGEPGEIKTCGRMRAANIVGDRVDVSMGNPHCVIFVEV"
BASE COUNT          213 a          339 c          373 g          171 t
ORIGIN      
        1 ggactcggct acgcggaagc gctcgcctgg tcgcgcggcg agatgacacg tcgcgaggcg
       61 atcgcgtcga cgacgcagcg cacgatccgc tacgcgaagc ggcagcggac gtggttccgt
      121 cgcatggatc cggcgctgcg cgtcgatgcg gccgatgcgc gcgcggcgac ggaggccatc
      181 attcgcatgg caagggaaag catcgcgcgt gcgtgaaccc atgtcaccgc gcgccggagc
      241 gcgcatcgca tagatagaga aagcatcgga gtcaaaaagg cgtgaagaac aatctgccgc
      301 tgcaagacgc gtatttgcag gcgatgaaga gagagaacgc gccggtgacg atctacctcc
      361 gcaacggtta tcagttgcgg ggtcacgtcc gcgggttcga cagcttcacg atcatcctgg
      421 aatacgataa caagccgcat ctcgtgtaca aacacgccgt gtcgacggtg tcgccgcaga
      481 cgccggtgca gttcaccggg ggcgatctga cggctttgcg tccggcgccg gtcgcggccg
      541 cggccgcggg tgccgaacaa cccggcggtg tccccgaagg aatcgcgcgt acgtccgcat
      601 cttagcgcgc tcttcgcatg gccctgatct ccatcaccaa atgccaagga acaggcaatg
      661 atttcgtgct gctcgaccgc acggccggca gcgagcttgc ctatgaagac atagcgcaga
      721 cgatctgcga ccgccacctc ggggtcggtg cagacgggct gctagtcctc gagcgggcga
      781 agagccccgg cgcggacgtc gcgctgcgca tcttcaacgc ggacgggtcg gcggccgaca
      841 tgtgcggcaa cggcatccgc tgcgtcgcgc gctacatgca cgaaaggacg ccctcggcgc
      901 cgcgacgtct gcgtatcgaa acgccgtcag gcgacgtgcg aacggagatg gtgagcgact
      961 cgcctttctc agtgcgcgtc gacatgggcg aaccgggcga gatcaagaca tgcggtagga
     1021 tgcgggcggc gaatatcgtc ggcgatcgag tcgacgtatc gatgggcaat ccgcactgcg
     1081 tcatcttcgt cgaggt
//
LOCUS       DASUCJ010000165         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1346687, whole genome shotgun sequence.
ACCESSION   DASUCJ010000165 DASUCJ010000000
VERSION     DASUCJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346687"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFU90_02095"
     CDS             complement(<1..1140)
                     /locus_tag="VFU90_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5478588.1"
                     /translation="MATWADMPRSPGHAFYDRLQEVLREAGFDAFVEGVCKPHYAPRM
                     GAPSLPPGRYFRMHMIGYFEGIDSERGIAWRCADSFSLRDFLRLANVEKVPDHSWLSR
                     TRSRLAHETHETVFGWVLKLVAERGLVKGERIGVDGSTMEANAALRTIVRRDNGENYR
                     AMLTRMAQESGIETPSAEDLARFDRKRKGKTLSNADWKSPTDPDARIAKMKDGTTHLA
                     YKPEHAVDLDTGVVVAAPIHPADEGDTTTLSPTLEAAERNLDAVGLAPTEDEPCVLAA
                     DKGYHAREQLKALEGGLWKTRIAEPEPANGYHRWHGDEAARTAVYANRARLKSDIGRE
                     TMHRRGELVERSFAHILDRGGMRRAWLRGRENVHKRYLIHVAGFNL"
BASE COUNT          184 a          421 c          363 g          213 t
ORIGIN      
        1 gaggttgaag ccggcgacgt ggatcagata acgcttgtgc acgttctcgc gtccgcgcaa
       61 ccaggcccgt cgcatgccgc cgcggtcgag aatgtgagcg aacgaccgct cgaccagctc
      121 gccgcgacga tgcatggtct cgcgcccgat gtcggatttc aaccgcgccc ggttggcgta
      181 aaccgcggtg cgcgccgcct cgtcgccatg ccagcgatga tagccgttgg ccggctcggg
      241 ttcggcgatg cgcgtcttcc acaagccgcc ttcgagcgcc ttcaactgtt cacgcgcgtg
      301 ataccccttg tcggcggcca gaacgcaagg ttcatcctca gtcggggcca acccgactgc
      361 gtcgaggttg cgctcggcgg cttcgagggt cggcgaaagc gtcgtcgtgt cgccctcgtc
      421 ggccggatgg atcggcgcgg ccaccacgac gccggtgtcg agatcgaccg cgtgttcggg
      481 tttgtaagcc agatgcgtcg ttccatcctt catcttggcg atcctggcgt cagggtcggt
      541 cgggcttttc cagtcggcgt tcgacagcgt cttacccttg cgcttccggt cgaagcgcgc
      601 caagtcctcg gcgctcggcg tctcgatccc gctctcctgc gccatccgcg tcagcatcgc
      661 gcgatagttc tcgccgttgt cgcgccgcac gatcgtgcgc agcgcggcgt tggcttccat
      721 ggtcgacccg tcgacgccga tccgctcgcc cttcaccaag ccgcgctcgg ccacgagctt
      781 taaaacccag ccgaacacgg tctcgtgcgt ctcgtgcgcc agacgcgaac gcgtacgcga
      841 tagccacgaa tgatcgggga ccttctcgac gttcgccagc cgcaggaaat cgcgcagcga
      901 gaacgagtcc gcgcaacgcc aggcgatgcc gcgctcgctg tcgatcccct cgaaatagcc
      961 gatcatgtgc atccggaagt atcggcccgg cggcagggac ggcgcgccca tccgcggcgc
     1021 gtagtgaggc ttgcacaccc cttccacgaa cgcgtcaaac ccggcttccc gaagcacctc
     1081 ctgcaagcga tcgtagaacg cgtgtcccgg cgagcgcggc atatccgccc acgtcgccat
     1141 caggtcatcc tggaccgctt ccgatcgccc catcgccatc g
//
LOCUS       DASUCJ010000166         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1086949, whole genome shotgun sequence.
ACCESSION   DASUCJ010000166 DASUCJ010000000
VERSION     DASUCJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086949"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFU90_02100"
     CDS             complement(<1..975)
                     /locus_tag="VFU90_02100"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478589.1"
                     /translation="MSRSIRTALRLATAVPLLVAIAVSAHAQQSKSRARKSEKAANAA
                     EAANGAAPDSAFLATLKWRFIGPEGNRTDAIAGVPGDPNTYYAGAASGGVWKSTDGGA
                     HWTPIFDDQPVQSIGSIAVAPSDPNVVYVGTGEPYLRSHVSVGWGMFRSTDAGKTWSR
                     AGLENTGRISRITIDPSDPNRVYVAALGTTYGPEADRGVFRTTDGGKTWEKVLFVNDS
                     TGAVDVLLDPKNSRTLYASTWQIATHTWGRESGGAGSGIWKSTDGGTTWSRLTGHGLP
                     TRPVGKVTLGLSPANTQRIYAQIETGDGIPWHGAPTDTGRLWRSDDAGA"
     gene            complement(1032..>1234)
                     /locus_tag="VFU90_02105"
     CDS             complement(1032..>1234)
                     /locus_tag="VFU90_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478590.1"
                     /translation="AEGTAAAPPPNGKAPAPSAGVASLIEQAQSHYDRAIAAQRAGDW
                     SAYGKQIDSLGAVLTQLRSHHP"
BASE COUNT          187 a          418 c          420 g          209 t
ORIGIN      
        1 ggcaccggcg tcatccgagc gccacagacg cccggtatca gtgggcgcgc cgtgccacgg
       61 aatgccgtcg ccggtctcga tctgcgcgta aatgcgctgc gtgttggccg ggctcaagcc
      121 taacgtcact ttgccgacgg gccgagtggg gaggccgtgg cccgttaggc gcgaccaggt
      181 ggtcccgccg tcggtcgact tccagatgcc gctgcccgcg ccgccgctct cgcggcccca
      241 cgtgtgcgtc gcgatctgcc acgtgctcgc gtacaacgtc cgcgaattct tcgggtcgag
      301 cagcacgtcc accgcgcccg tcgaatcatt cacgaacagc accttctccc acgtcttgcc
      361 gccgtcggtc gtgcggaaca ccccgcgatc cgcctccgga ccgtacgtgg tgccgagtgc
      421 cgcgacgtat acacggttgg gatcggatgg atcgatggtg atgcgcgaga tgcgtcccgt
      481 gttctcgagt cccgcgcgcg accatgtttt gccggcgtcc gtcgaccgga acatgcccca
      541 cccgaccgac acgtggctcc gcaaatacgg ctcgccggtg ccgacgtaca cgacattggg
      601 atcggacggc gcgaccgcga tcgatccgat ggattgcacc ggctgatcgt cgaaaatcgg
      661 cgtccagtgc gctccgccgt cagtgctctt ccaaacgccg ccgcttgcgg cgccggcgta
      721 gtacgtgttc ggatcgccgg gcacgcccgc gatcgcgtcg gtgcgattgc cttcggggcc
      781 gatgaaacgc cacttgagcg tcgcaagaaa ggcagagtcg ggggcggcgc cgttggccgc
      841 ctccgccgca ttcgcagctt tctctgactt cctggctcgt gatttcgatt gctgagcgtg
      901 cgccgagaca gcgatggcca cgaggagcgg gacggccgtg gccaggcgca gggcagtgcg
      961 gatggagcgc gacatcgagc gaacctcgag gatggatgtg ggcgggaacg agcaacagcg
     1021 gcaatcacta actatggatg atgcgagcgc aactgcgtga gcacggcgcc gagcgagtcg
     1081 atctgcttgc cgtatgccga ccagtctccg gcgcgctgcg cggcgatcgc ccgatcgtag
     1141 tgcgactgcg cctgttcgat cagcgacgcg acgcccgcgg atggcgccgg cgccttgccg
     1201 ttaggcggcg gcgcggcggc cgtgccctcc gcct
//
LOCUS       DASUCJ010000167         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1147825, whole genome shotgun sequence.
ACCESSION   DASUCJ010000167 DASUCJ010000000
VERSION     DASUCJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147825"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFU90_02110"
     CDS             complement(<1..487)
                     /locus_tag="VFU90_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5478591.1"
                     /translation="MKVLVTGGAGYVGSTLVPELLAAGHSVRVLDSLLHGGQSLLPVW
                     THPRFEFVRGDVRDEPIVRQAVAGMNAVVHLAAIVGDPACARQPDTARDVNQVASLAL
                     LDAARRAGVGRFLFASTCSNYGKMADADGYVDESSDLRPVSLYAETKVAVERAMLEDG
                     ID"
     gene            complement(487..>1571)
                     /locus_tag="VFU90_02115"
     CDS             complement(487..>1571)
                     /locus_tag="VFU90_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5478592.1"
                     /translation="SAHVISDDRMEQRLQDATVAPGTPLTDALKRLDANGMGVLLLAD
                     LDRRLVGIITDGDVRRHILSGASLSVSCESIASRHPRVANRGMTTAELLDLMGGSRDD
                     TVDHLPVLTDDGIVVGLVLRRDLVAPDDTFTAVIMAGGFGKRLMPLTTDTPKPLLPVG
                     DRPLMERTIERLRRAGIRRVNVATHYLSDKISNHFGDGREFGVEMRYVTEDRPLGTAG
                     ALRLMEPPAGPLLVVNGDILTGVNYQDMLAYHRENGAIATVGVRKYDLEVPYGVIDCS
                     GVSVSALREKPVQQFLVNAGMYLLDPAAVAYIPAGERFDMTDLIQRLLAEGRKVVAFP
                     VVEYWLDIGKPADYERAQKDVLQVRA"
BASE COUNT          255 a          523 c          540 g          253 t
ORIGIN      
        1 cgtcgatccc gtcctcgagc atcgcgcgtt cgacggcgac cttggtctcg gcgtagagcg
       61 acaccggccg caggtccgac gattcgtcga cgtatccatc ggcgtcggcc atcttgccgt
      121 agttgctgca ggtagacgcg aagaggaagc ggcccacgcc ggcgcggcgc gccgcatcga
      181 ggagcgcgag cgacgcgacc tggttgacgt cgcgcgccgt atcgggttgt ctcgcgcagg
      241 ccgggtcgcc gacgatggcc gcgaggtgga ccacggcgtt catgcccgcc accgcctgcc
      301 taacgattgg ctcgtcgcgc acgtcgccgc gcacgaactc gaagcgcggg tgcgtccaca
      361 ccgggagcag cgactgtccg ccgtgcagca agctgtccaa cacgcgcacc gagtgtccgg
      421 ccgcgagcag ctcgggcacc aacgtcgatc cgacgtaccc ggcacccccg gtcacaagaa
      481 ccttcatcac gccctcacct gaagcacgtc cttctgggca cgctcgtagt ctgccggctt
      541 tccgatgtcc aaccagtatt ccaccaccgg gaaggcgacg accttccgcc cctcggccag
      601 caatcgttga atgagatccg tcatgtcgaa gcgttccccg gcggggatgt acgcgacggc
      661 agctgggtcg agcagataca tcccggcatt cacgaggaac tgctgcaccg gcttttcgcg
      721 cagcgcggaa acggacacgc cggagcagtc gatcaccccg tacggcactt cgaggtcgta
      781 tttgcgtacg ccgacggtcg cgatcgcgcc gttttcgcga tggtaggcga gcatgtcctg
      841 atagttcacg ccggtcagga tgtcgccgtt gacgacgagg agcggtccgg cgggcggttc
      901 catgaggcgc agcgcgccgg cggtgcctaa cgggcggtcc tcggtcacgt agcgcatctc
      961 gacgccgaac tcgcggccgt cgccgaaatg gttgctgatc ttgtcgctca ggtagtgcgt
     1021 ggcgacgttc acgcggcgga tgcccgcgcg gcggaggcgc tcgatggtgc gctccatgag
     1081 cggacggtcg cccaccggca acagcggctt gggcgtgtcc gtggtgagcg gcatgaggcg
     1141 cttgccgaag ccgcccgcca tgatgacggc cgtgaaggtg tcatcggggg cgacgaggtc
     1201 gcgccgcagc acgaggccca cgacgatgcc gtcgtccgtt aggaccggca gatggtccac
     1261 cgtgtcatcg cgcgagccgc ccatgaggtc gagcagctcg gcggtcgtca tgccgcggtt
     1321 ggcgacgcgc gggtggcgcg aggcaatgct ctcgcacgat acggagagtg acgcgccgga
     1381 gaggatgtgc cggcggacat caccgtcggt gatgatgccc acgagacggc gatccaagtc
     1441 ggcgagcagc agcacgccca tgccgttcgc atcgaggcgt ttgagcgcat ccgttagcgg
     1501 cgttcccggc gcgacggtgg catcctgcag tcgctgctcc atccgatcgt cggagatcac
     1561 gtgagcgctc c
//
LOCUS       DASUCJ010000168         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_368538, whole genome shotgun sequence.
ACCESSION   DASUCJ010000168 DASUCJ010000000
VERSION     DASUCJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368538"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="icd"
                     /locus_tag="VFU90_02120"
     CDS             complement(<1..1191)
                     /gene="icd"
                     /locus_tag="VFU90_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5478593.1"
                     /translation="MDRRRHRLDVPVAVHDTRRHFSGVPTHTDDCPGSIMSTSTVSEY
                     KHLAPADGVPITMRDGKLSVPTEPIIPFIEGDGTGPDIWRASQFVLDGAVRKAYGSKR
                     RIAWFEVLAGEKSKNVLNTWLPEETVAAISKYLVAIKGPLTTPVGGGFRSLNVALRQQ
                     LDLYACVRPVRWFEGVPSPVKAPQDVNMVIFRENTEDIYAGIEYKARSPEAEKLIRFL
                     REELGARTIRFPDSSGIGIKPISEDGTKRLVRAAIDYAIAQRHPSVTLVHKGNIMKYT
                     EGAFRDWGYETARQEYGATELDGGPWLKLPNGLVIKDVIADAFLQQILLRPKEYSVIA
                     TPNLNGDYISDALAAQVGGIGIAPGANINYLTGHAVFEATHGTAPKYAGQDKVNPGSL
                     ILSAE"
     gene            1147..2877
                     /gene="aceK"
                     /locus_tag="VFU90_02125"
     CDS             1147..2877
                     /gene="aceK"
                     /locus_tag="VFU90_02125"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEU5478594.1"
                     /translation="MNGNGNVEAVATAIHEAYDAYQSRFRAITRRASEHFVRREWREA
                     QRDASERIVLYKQHVDMVLAQTRAMLGDCVTDVTTWEEIKWMHGALIAERADCEIAES
                     FFNSVTRRIFSTVGVNPLVEYVFPDNEPGDIDDGPPVTRRYAWRDDTADVVRRMLHDL
                     SWDVARWRDMDGDAERVAERIDAELLTLRGALGFDAIEVLEAPFFRNKGAYLVGRLLR
                     DDDKPALPLLLPLLNGDEGIYVDAVLLSEDEASIVFGFSWSYFLVEIARPHAVVDFLH
                     GIMPLKRIDELYNSIGFNKHGKTELYRTLVAHLAAPGARFEFAPGEQGLVMSVFTLPS
                     LNVVFKIIRDRFGHPKRTTRRRVMDKYQFVFVRDRVGRLADAQEFEHLDLPRGCFSEP
                     LLAHLLDAAPSSVELRGDHVVVRHLYTERRVTPLNVYLRDESPYDAALAMLDYGTAIK
                     DLAAANIFTGDMLMKNFGVSRHGRVIFYDYDELSLLTDCVFRRLPQPQTPEQEMAAEP
                     WYYVGENDVFPEEFEAFMVPPGKLRKRFLQRHRDLFTVEFWQSMQERQRRGDMPDVFP
                     YPESRRLRHT"
BASE COUNT          490 a          940 c          968 g          537 t
ORIGIN      
        1 ctcggccgag aggatcagcg agccggggtt gaccttgtcc tggccggcgt acttcggcgc
       61 cgtcccgtgg gtcgcctcga acaccgcatg gccggtgaga tagttgatgt tcgcgccggg
      121 cgcgatcccg atgccgccca cctgcgctgc caacgcgtcg gagatgtagt cgccgttcag
      181 gttgggcgtc gcgatcacgc tgtattcctt gggacgcagc aggatctgct gcaggaacgc
      241 gtcggcgatc acgtccttga tcaccagtcc gttaggcagc ttgagccacg ggccgccatc
      301 cagctccgtg gcgccgtatt cctgccgcgc cgtctcgtag ccccagtcgc ggaacgcgcc
      361 ttccgtgtac ttcatgatgt tgcccttgtg caccaaggtc acgctcggat gccgctgcgc
      421 gatggcatag tcgatcgccg cccgcaccag acgcttggtg ccgtcttccg agatcggctt
      481 gatgccgatg ccgctggagt ccggaaagcg gatggtgcgc gctcccagct cctcgcgcag
      541 aaaccggatg agcttctcgg cttccggaga gcgcgccttg tactcgatgc cggcgtagat
      601 gtcttccgta ttctcccgga agatcaccat gttcacatcc tgcggcgcct tgaccggtga
      661 gggcacgccc tcgaaccagc gcacgggtcg cacgcaggca tacagatcga gttgctggcg
      721 cagcgcgacg ttgagcgagc ggaatccgcc gccgaccggc gtcgtgagcg gtcctttgat
      781 tgcgaccaga tatttcgaga tcgcggccac cgtttcctca ggcagccacg tattgagcac
      841 attcttggat ttctcgccgg ccagcacctc gaaccacgca atgcgacgct tcgagccgta
      901 tgctttgcgg accgcaccat cgagcacgaa ttgcgaggcg cgccagatat cgggcccggt
      961 gccgtcgccc tcgatgaacg ggatgatggg ctcggtgggc acggacagct tgccatcacg
     1021 catggtgatc ggcacgccgt cggccggtgc aaggtgcttg tattcggaca cggtggaggt
     1081 cgacatgatg ctccctgggc agtcgtcggt atgcgttgga actcctgaaa agtgacgtcg
     1141 ggtgtcatga acggcaacgg gaacgtcgag gcggtggcga cggcgatcca tgaggcgtac
     1201 gacgcctacc agagccgctt tcgcgccatc acgcgacgcg cgtccgagca tttcgtgcgg
     1261 cgcgaatggc gcgaagcgca gcgcgatgca tcggagcgga tcgtgctcta caagcagcac
     1321 gtggacatgg tgctggcaca gacgcgcgcc atgttgggcg actgcgtcac cgacgtcacg
     1381 acgtgggaag aaataaagtg gatgcacggc gccctcatcg ccgagcgtgc cgactgcgag
     1441 atcgcggaga gttttttcaa ttcggtgacg cggcgcattt tctcgacggt cggtgtgaac
     1501 ccgttggtcg aatacgtttt ccccgacaac gagcccggcg acatcgacga tgggccgccg
     1561 gtcacgcggc gctatgcgtg gcgggacgac acggccgacg tcgttcgacg catgctgcac
     1621 gatctgtcgt gggatgtggc gcggtggcgc gacatggacg gcgacgccga gcgggtcgcc
     1681 gagcgcatcg acgccgagct gctcacgctg cggggtgcgt taggcttcga cgcgatcgag
     1741 gtgctcgagg cgccgttctt ccgcaacaaa ggcgcctatc tcgtcggccg cttgctgcgc
     1801 gacgacgaca agccggcgct cccgctgctg ctgccgctgc tcaacggcga cgagggcatc
     1861 tacgtggatg cggtgctgct gagcgaggac gaggcgagca tcgtcttcgg gttcagttgg
     1921 tcgtatttcc tcgtcgagat cgcgcgcccg cacgcagtgg tcgattttct ccacgggatc
     1981 atgccgctca agcgcatcga cgagttgtac aactcgatcg ggttcaacaa gcacggcaag
     2041 accgagctct atcggacgct ggtcgcgcac ctcgcggcgc ccggcgcgcg tttcgagttc
     2101 gcgcccggcg agcagggcct cgtgatgagc gtgttcacgc tgccgtcgct caacgtcgtg
     2161 ttcaagatca tcagggaccg cttcggccat ccgaagcgca ccacccggcg gcgcgtgatg
     2221 gacaagtacc agttcgtgtt cgtgcgcgac cgcgtgggcc gcctggcgga cgcccaggag
     2281 ttcgagcacc tcgacctgcc gcgcggctgc ttcagcgagc cgctactggc gcacctgctc
     2341 gatgcggcgc cgtcgagcgt cgagctccgc ggcgaccacg tcgtcgttag gcatctgtac
     2401 accgagcggc gcgtcacgcc gctcaacgtc tacctgcgcg atgagtcgcc gtacgacgcg
     2461 gccctcgcga tgctcgacta tggcaccgcc atcaaggatc tcgccgccgc caacatcttc
     2521 accggcgaca tgctgatgaa aaatttcggc gtgagccgcc acggacgcgt gatcttctac
     2581 gactacgacg agttgtcgct cctcaccgac tgtgtcttcc ggcgcctgcc gcagccgcaa
     2641 acgcccgagc aggagatggc ggcggaaccc tggtactacg tgggcgaaaa cgacgtcttc
     2701 ccggaagagt tcgaggcatt catggtgccg cccggcaagc tccgcaagcg attcttgcag
     2761 cggcatcgcg atctgttcac cgtcgagttc tggcaatcga tgcaggaacg gcagcgtcgc
     2821 ggcgacatgc cggacgtctt cccgtacccc gagtcgaggc ggctccgtca cacgtgagcg
     2881 cgagcgcgtg cgacgtgatg gtgatcggcg ccggggtaat gggaagcgcc gcggc
//
LOCUS       DASUCJ010000169         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_185064, whole genome shotgun sequence.
ACCESSION   DASUCJ010000169 DASUCJ010000000
VERSION     DASUCJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185064"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VFU90_02130"
     CDS             <1..1205
                     /locus_tag="VFU90_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478595.1"
                     /translation="PLLPFYAVKFGASDFMVMVLVSAFSAMQLVSAPLWGRVSDRYGR
                     KPALLIGLGASAIAYVVFAYADSYWLLLLSRAVQGAGGGTTAVIQAYVTDSVEPRNRA
                     KGLGWLSAATNAGVVLGPAIGSWSQGFNNHAPGLVAAVLCLLNVLFAARFLVESRVVS
                     AADRAKPKTGSKTSTAVLRVASWRHMGDPPSRLIWMYSVGIGAFYGVNALITLYLGRA
                     FNVTAESIGPFYSYIGALNILFRLLVLGKAVDGLGEMRTSRIGATALALGLAFIPFTS
                     PVHIAHLTYLTIGLALALIPFGTALNFPCVTATLSRVVKDEDRGLYLGVQQTYGGLAR
                     VLYPLAAGAASDHFGVGAPFWLSAILVASTILIGRKLDRFTHYTGEHPLVSPIVEQAV
                     KETPIIAE"
     gene            complement(1231..>2262)
                     /locus_tag="VFU90_02135"
     CDS             complement(1231..>2262)
                     /locus_tag="VFU90_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5478596.1"
                     /translation="PYIFGGGLRVLSTQIVASKLNGAMDLAYVETTVLAVGAIAALIL
                     LRWFERRRTYAVSSKGAVVRARLHSPTARLLAPIVGAMLVVILILPHAMVILVSFARD
                     GTWTTQVLPPEYTLDNFRRLATDSDLLVPIWNSVSMTAIATALDIVVCFVAAYLLVLR
                     RFRVRWLLAILVALPWAIPGTAIAIGLASTFDRTSLASARVLLVGTFWILPLAYFVRG
                     VPLVSSATEGSLRQMDPSLEDAARGLGASWWLTMRRVVLPAARPGLVAGAMLAAITAV
                     GEFVASIVLFTHANRPISMEILSQLRNFAFGTAAAFSVVLIALVLAITLGARWAEGRW
                     SRAEAVVIQ"
BASE COUNT          402 a          752 c          737 g          371 t
ORIGIN      
        1 tcccgttgtt gccgttctac gccgtcaagt tcggcgcgag cgacttcatg gtgatggtgc
       61 tcgtgtcggc gttctcggcc atgcagctgg tgagcgcgcc gctctgggga cgggtgtccg
      121 accgatacgg acgcaagccg gcgctcctca tcggactcgg cgcgtcggcg atcgcatacg
      181 tcgtcttcgc gtatgcggat tcgtactggc tgctgctgtt gtcgcgcgcc gtgcagggtg
      241 cgggcggcgg cacgacggca gtgattcagg cctacgtcac cgacagcgtc gagccgcgca
      301 atcgcgccaa ggggttaggc tggctctcgg cggcgaccaa cgccggcgtc gtgttaggcc
      361 cggccatcgg ctcgtggtcg cagggattca acaaccacgc gccgggcctg gtggcggcgg
      421 tcctctgcct gctcaacgtg ctgttcgccg ccagattcct ggtcgagtcg cgcgtcgtct
      481 cggcggccga tcgcgcgaag ccaaagaccg gcagcaaaac gtcgaccgcc gtgctgcgcg
      541 tcgcatcgtg gcgccacatg ggcgatccgc cgtcgcggct gatctggatg tactcggtag
      601 gcatcggcgc attctacggt gtgaacgccc tgatcacgct ctacctcggc cgagcgttca
      661 atgtaaccgc tgaatcgatc gggccgttct actcgtacat cggcgcgctg aacatcctgt
      721 tccggctgct tgtgttaggc aaggccgtgg atgggctcgg cgaaatgcgg acgtcgcgca
      781 tcggggcgac cgcgctcgcg ctggggctgg cgttcatccc gttcacatcg ccggtgcaca
      841 tagcccattt gacgtacctg acgatcggtc tggcgctcgc cctcatcccg tttggcaccg
      901 cgctcaactt cccatgcgta acggcgacct tgtcacgcgt ggtgaaggat gaggatcgcg
      961 gtctgtatct cggcgtgcag cagacgtacg gcggcttagc gcgcgtgctg tatccgttag
     1021 ccgccggcgc cgcgtcagat cacttcggtg tcggcgcgcc gttctggttg tcggcgatat
     1081 tggtcgcgag cacgattctc atcggcagaa agctggatcg cttcacacac tacacgggcg
     1141 agcatcccct ggtttcaccg atcgtcgagc aagccgtgaa agaaacgccg atcatcgccg
     1201 agtaacagtc tcgcgcgcgc cgccccgccg ctactgaatt acgaccgcct ctgcgcgcga
     1261 ccaccggccc tccgcccagc gcgcaccaag cgtgatcgcg agcacgagcg cgatcagcac
     1321 gacgctgaac gccgcggcgg ttccaaacgc gaagttccgc agttgggaca ggatctccat
     1381 cgaaatcggc cgattggcgt gcgtgaagag cacaatgctc gcgacgaatt cgccgaccgc
     1441 cgtaatcgcg gcgagcatcg cgcccgcgac caagccgggg cgcgcggcgg gcagcaccac
     1501 gcggcgcatg gtgagccacc agctcgcgcc taacccgcgc gccgcatcct cgagcgacgg
     1561 atccatctgg cgcaacgacc cttccgtcgc gctggacacg agaggcacgc cgcggacgaa
     1621 gtacgcgagc ggcagaatcc agaacgttcc gaccaacagg acccgcgcgc ttgcgagcga
     1681 cgtgcgatcg aaggtcgatg caagcccgat ggcgatcgcc gtgccgggta tggcccaagg
     1741 cagagccacc agaatcgcca acagccatcg cacgcgaaat cggcgaagga ccagcaggta
     1801 cgcggcgacg aagcacacca cgatgtcgag cgccgtggcg atcgccgtca tcgacacgct
     1861 attccagatc ggcaccaaca gatcgctatc ggtggcgagg cggcggaaat tgtcgagcgt
     1921 atactccggc gggaggacct gcgtcgtcca tgtgccgtcg cgcgcgaacg agacgaggat
     1981 gaccatcgcg tgaggcaata tcagaatcac gaccagcatc gcgccgacga tcggcgcaag
     2041 caggcgcgcc gttggcgaat ggagacgcgc gcggacaacc gcgcctttcg acgacacggc
     2101 gtacgtccgc cgccgctcga accaacgcag gagaatgagc gccgcgatcg caccgaccgc
     2161 gagcaccgtc gtctcgacgt acgcgagatc catcgcgccg ttgagcttcg acgccacgat
     2221 ttgcgtcgag agcacgcgca ggccgccgcc gaagatgtat gg
//
LOCUS       DASUCJ010000170         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1453626, whole genome shotgun sequence.
ACCESSION   DASUCJ010000170 DASUCJ010000000
VERSION     DASUCJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453626"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFU90_02140"
     CDS             <1..778
                     /locus_tag="VFU90_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5478597.1"
                     /translation="HNRAMTDFCSSDTRLLPVGYVPLADAERACAAAREAIEIGCKAI
                     LVPSAPPRDKSPAHPDYFPMWRHLEETGVPFMLHIGGGGRPLRRAWHVNGKPPTTDFL
                     GGGENIRSKDYMVLHQPPETFLACMVLDGVFEEFPGLRGGCIEQGALWVVPLLQRLDI
                     AQATFQRTEPALKLPLRASDYLRRQVKFTPFPTEPVGWMIEQAGPELFLFSSDYPHPE
                     GGRDPVARFEASMEGVSDDARDAFYASNFAAMMGPMLLAS"
     gene            complement(727..>1159)
                     /locus_tag="VFU90_02145"
     CDS             complement(727..>1159)
                     /locus_tag="VFU90_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478598.1"
                     /translation="TTRSSSACGSPLWPAIGLVDAMRVGPDVGAHWAARGVGGVEQRS
                     ISCLDNTWRAARLRTMFHGALWANDPDCVLLRRRNTKLWASDRIGWARWVANSGQLLM
                     DGDRLDDLAPRDFAFWEQLAATRGDQDASSIGPIIAAKLDA"
BASE COUNT          184 a          418 c          378 g          179 t
ORIGIN      
        1 gcacaaccgc gcgatgaccg acttctgttc gtccgacacc cgcctgctcc ccgtcgggta
       61 cgtgccgctc gccgacgccg aacgcgcgtg cgcggcggca cgtgaagcga tcgagattgg
      121 ctgcaaggcg attctcgtgc cgtcggcgcc gccgcgcgac aaatcacccg cccatcccga
      181 ctacttcccg atgtggcgcc acctcgaaga aacgggcgtg ccgttcatgc tgcacatcgg
      241 cggcggcggc cggccgctgc gccgcgcctg gcacgtcaac ggcaagccgc cgaccaccga
      301 cttcctcggt ggcggcgaga acatccgctc gaaggactac atggttctgc accaaccgcc
      361 ggagaccttc cttgcgtgca tggtgctcga cggtgtgttc gaggagttcc cgggtctgcg
      421 cggcggctgc atcgagcaag gcgcgctgtg ggtcgttccg ctcttgcaac gtctcgacat
      481 cgcgcaggcg acgttccagc gcaccgaacc ggcgttgaag cttccgttgc gagcgagcga
      541 ctacctgcgg cggcaggtga agttcacgcc gttccccacc gaaccggtcg gctggatgat
      601 cgagcaggcc ggaccggaac tgttcttgtt ctcgtccgac tacccgcatc ccgaaggtgg
      661 ccgcgacccc gtcgcccgtt tcgaagcatc gatggaaggc gtgagcgacg acgcacgcga
      721 cgcgttctac gcgtccaact tcgccgcgat gatgggcccg atgctgctcg catcctgatc
      781 gcctcgcgtc gccgcgagct gctcccagaa cgcgaagtcc cggggagcga ggtcgtcgag
      841 ccggtcaccg tccatgagca actggccgga gttcgcgacc cagcgcgccc agccgatccg
      901 gtcgctcgcc cacaacttcg tgttgcggcg ccggagcagg acgcagtcgg gatcgttggc
      961 ccagagcgcg ccgtggaaca tggtgcgtaa gcgcgccgcc cgccacgtgt tgtcgagaca
     1021 cgagatcgac cgttgctcga ccccgccgac gccgcgcgcc gcccagtggg cgccgacgtc
     1081 cggaccgacg cgcatcgcgt cgacgagccc gatcgcgggc cacagtggag atccgcacgc
     1141 cgacgacgaa cgcgtcgtc
//
LOCUS       DASUCJ010000171         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1209229, whole genome shotgun sequence.
ACCESSION   DASUCJ010000171 DASUCJ010000000
VERSION     DASUCJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209229"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..585
                     /locus_tag="VFU90_02150"
     CDS             85..585
                     /locus_tag="VFU90_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEU5478599.1"
                     /translation="MRRSGTSWWLAFLPLLTGACIYGFAGGGLPPRIKSVAVLPFDNQ
                     TAAATLQSELYTEMRHDVESRLGLHEAAEDKADAVVKGTITKYEPDIPVAYSADPTQA
                     NSAQRKVQITVDVDIIDQATGRSIWSQKGMMRDGAYSQRDEASGRKEAIQKIVNDVVA
                     GAQSQW"
     gene            615..944
                     /locus_tag="VFU90_02155"
     CDS             615..944
                     /locus_tag="VFU90_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478600.1"
                     /translation="MTVGCLTLILLLIILAYVGNAVGQVYWRTYRFQDAMTQQAKFAA
                     GVADDTIIARLRAQADSLGLPPEAQRIHVRRTAKGINIWSQYADSVFLPGMNREVDFT
                     VHAENSF"
     gene            989..1420
                     /locus_tag="VFU90_02160"
     CDS             989..1420
                     /locus_tag="VFU90_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5478601.1"
                     /translation="MAWRAAAGRVAFTNGVFDLLHRGHVDLLCAARATADALIVGINS
                     DDSVRRLKGAGRPVRSEADRAYLIAALECVDAVVIFPEDTPLAIIRALHPDVLIKGGD
                     YAPADVVGRADVEGWGGRLVIVPLTAGHSTTATIASLRDHS"
BASE COUNT          250 a          489 c          470 g          235 t
ORIGIN      
        1 accggcacgt ggtgtatgaa cggacggacg cgcaccgatc gctgaatctc acggtggtcc
       61 gcgtagacac tgtctccaat ttcgatgcgt cgatctggca ctagttggtg gctcgcgttt
      121 ctcccccttc tcacgggagc ctgcatctac ggcttcgcgg gcggcggctt accgccgcgc
      181 atcaagagcg tagccgtgct gccgttcgac aaccagacgg ccgcggcgac gctgcaaagc
      241 gagctctaca ccgagatgcg ccacgacgtc gaatcgcgtc tcggtctgca cgaagcggca
      301 gaggacaaag ccgacgccgt cgtgaagggc acgatcacga aatacgagcc cgacatcccg
      361 gttgcctaca gcgccgatcc gacgcaagcc aactctgccc aacggaaggt gcagatcacg
      421 gtcgacgtcg acatcatcga ccaggccacc gggcgttcca tctggtcgca gaagggcatg
      481 atgcgcgatg gcgcgtacag ccagcgcgat gaagcctcgg gccgcaagga agccatccag
      541 aaaatcgtca acgacgtcgt ggcgggcgcg caatcgcaat ggtgatccga tccgcgggcg
      601 cgccgcgagg gcgcgtgacc gtcggatgtc tcacgctcat cttgctcctc atcattctcg
      661 cgtacgtggg gaatgcggtg gggcaagtgt attggcgcac ctatcggttc caggacgcga
      721 tgacgcagca ggcgaagttc gcggccggcg tggccgacga cacgatcatc gcgcggctcc
      781 gcgcgcaggc ggactcgctg ggtctcccac ccgaagccca acgcattcac gtgcggcgca
      841 cggcgaaggg catcaacatc tggtcgcagt acgcagactc cgtgttcctg cccgggatga
      901 accgcgaggt cgacttcacc gtgcacgccg agaattcgtt ctgagcgtgc ggccgccggc
      961 atcgaaggtc ctctcgttag gcgacgccgt ggcgtggcgc gccgccgccg gtcgcgtcgc
     1021 gttcaccaac ggcgtattcg acctgctcca tcgcggacat gtcgatctgc tctgcgcggc
     1081 gcgcgcaacc gccgacgcgc tcatcgtcgg catcaacagc gatgattcgg tgcggcgcct
     1141 caagggcgcg gggcggccgg tgcgctccga agccgaccgc gcgtacctga tcgcggcgct
     1201 cgagtgcgtc gacgccgtgg tgatcttccc ggaagacacg ccgctcgcga tcattcgggc
     1261 gctgcatccc gacgtgctca tcaagggcgg cgactacgcg ccggccgatg tcgttgggcg
     1321 cgcggacgtc gaagggtggg gcggtcgtct cgtgatcgtg ccgctcaccg ccggacactc
     1381 caccactgca accatcgcat cgcttcgtga ccacagctga tgtccccgcg ccgcgcaccg
     1441 gccg
//
LOCUS       DASUCJ010000172         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_139514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000172 DASUCJ010000000
VERSION     DASUCJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFU90_02165"
     CDS             complement(<1..1170)
                     /locus_tag="VFU90_02165"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478602.1"
                     /translation="MQSIGAIAVAPSNPNVVYVGTGESDMRSDIVQGDGMYKSVDAGK
                     TWTHVGLADSQQISIIRVDPHNPDHVFAAVLGHPYGPNAERGVFRSLDGGKTWKKVLG
                     PNDDTGAIDIIFKPGDSNVIYASLWQTRRPPWNIYPPSNGPGSGLYKSTDGGNTWTHI
                     QGNGFPSKNLGRIGIAISAAAPERIYAIVDAEQGGLYRSDDAGKTWKKMSGDPRIWQR
                     GWYFGQITADPKNPDRVYAMNTIELRSDDGGKAFLPVKGDPTGDDYHELWIDPNDPDR
                     QIMGVDQGAIITVNGGKTWSSWYNQPTAQMYHVSTDNRFPYRVYGAQQDSGAAAVPSR
                     STYEATINMMLFHEVTAGGESGMIAPDPNDPDIVYGGTVEKLDLKTQQTRSVDPTL"
BASE COUNT          184 a          371 c          400 g          241 t
ORIGIN      
        1 gagcgttgga tcgacactgc gcgtctgttg ggtcttgagg tccagcttct cgacggtgcc
       61 gccgtacacg atgtcggggt cgttcggatc gggcgcgatc atgccgctct caccacccgc
      121 ggtgacctcg tggaacagca tcatgttgat cgtcgcttcg taggtcgagc ggctgggcac
      181 ggcggccgca ccggaatcct gctgcgcgcc gtacacgcga tacggaaagc ggttgtcggt
      241 gctgacgtgg tacatctgcg cggtaggctg gttgtaccag ctcgaccagg tcttgccgcc
      301 gttcacggtg atgatcgcgc cctggtcgac gcccatgatc tggcgatcgg gatcgttcgg
      361 gtcgatccac agttcgtggt aatcatcgcc ggtcgggtcg cccttcaccg gcaggaaggc
      421 cttgccgccg tcgtccgaac gcagctcgat ggtgttcatc gcgtagacgc ggtcgggatt
      481 cttcggatcg gcggtgatct gcccgaagta ccagccgcgc tgccagatgc gcggatcgcc
      541 gctcatcttc ttccaggtct tgccggcgtc gtcggagcgg tacaggccgc cctgctccgc
      601 atcgacgatc gcgtagatgc gttcgggcgc agcggcggaa atcgcgatgc cgatgcggcc
      661 gagatttttc gacgggaaac cgttgccctg gatgtgcgtc caggtgttgc cgccatcggt
      721 ggatttgtac aaaccggaac ccggcccgtt cgacggcgga tagatgttcc acggcggacg
      781 ccgcgtctgc cacagcgacg catagatcac gttggaatcg cccggcttga agatgatgtc
      841 gatggcgccg gtgtcgtcgt tcgggcccag taccttcttc caggtcttgc cgccgtccag
      901 cgaacggaac acgccgcgtt cggcattcgg accgtaggga tgcccgagca ccgccgcgaa
      961 cacgtggtcc gggttgtgcg gatcgacgcg gatgatcgag atctgctgcg aatcggcgag
     1021 gccgacgtgc gtccaggtct tgccggcatc gacggacttg tacatgccgt cgccctgcac
     1081 gatgtccgag cgcatgtcgg attcgccggt gccgacgtag acgacattcg ggttcgacgg
     1141 cgccaccgcg atcgcgccga tcgactgcac gggttccttg tcgaacaacg gctgcc
//
LOCUS       DASUCJ010000173         1360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_735471, whole genome shotgun sequence.
ACCESSION   DASUCJ010000173 DASUCJ010000000
VERSION     DASUCJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735471"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFU90_02170"
     CDS             <1..605
                     /locus_tag="VFU90_02170"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5478603.1"
                     /translation="AARLNERGLRFVDAPVSGGPVRAADGTLAIMVGADPADFERAEP
                     LLRAMGTPHHLGPVGMGETVKLVNQIIIGNVMIANVEALLFAQRAGADLAQVREVLAT
                     ATASNYVLEQWLPKTWLAGKLDGGFALDLLRKDLAAALDAARVADYPMPATALAYQVY
                     TSRSAQGDGALDYSAVAKSYERIAGDEARSGNVRQESGLQ"
     gene            559..1281
                     /locus_tag="VFU90_02175"
     CDS             559..1281
                     /locus_tag="VFU90_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5478604.1"
                     /translation="MKPARGTFVKSPDFNKKILIVDDESAILQTLRFNLERSGYAVVT
                     ASDGRSAIAMAQREQPDLVVLDIMLPVLDGIEACKEIRKFSSVPIIMLTAKDQEIDKV
                     LALELGADDYVTKPFALHEFLARVKARLRRQAPIQSGHDGAIALGEIVLDPSRQQLTV
                     RGKNVALAPKEFALLRVLMENNGRVVTRQTLLDKVWGYDFEGEQQTVSVHIRWLREKI
                     EEDSGTPRHILTVRSRGYMFKS"
BASE COUNT          242 a          426 c          466 g          226 t
ORIGIN      
        1 tcgccgcacg gctgaacgaa cggggcctgc gcttcgtcga cgcgccggtc agcggcgggc
       61 cggtccgcgc cgccgacggg acgctcgcga tcatggtcgg ggccgacccg gcggacttcg
      121 agcgcgccga gccgctgctg cgcgcgatgg ggacgccgca tcacctggga cccgtcggaa
      181 tgggggaaac cgtcaagctg gttaatcaga tcatcatcgg caacgtgatg atcgccaacg
      241 tcgaggcact gctcttcgcg cagcgggccg gcgccgattt ggcgcaggtg cgcgaagttc
      301 tcgcaacggc taccgcctcg aactatgttc tggagcagtg gctgccgaag acgtggctgg
      361 cggggaagct cgacggcgga ttcgctctcg acctgctccg caaggatctt gccgccgcgc
      421 tcgacgccgc gcgcgtcgcc gactatccga tgcccgccac cgcgctggcg taccaagtgt
      481 atacttctcg ctccgcacag ggggacggcg cgctggatta cagcgcagtg gccaaatcgt
      541 atgaacggat cgctggagat gaagcccgct cggggaacgt tcgtcaagag tccggacttc
      601 aataagaaaa ttttgatcgt cgacgacgag tcggcgattc tgcagacgct gcgattcaac
      661 ttggaacgca gcggctacgc cgtcgtgacc gccagcgacg ggcgcagcgc gattgcgatg
      721 gcgcagcgcg agcagccgga tctcgtcgtg ctcgacatca tgctgccggt gctcgacggc
      781 atcgaagcgt gcaaggagat tcgcaagttc agctccgtcc cgatcatcat gctcaccgcc
      841 aaggatcagg agatcgataa ggttctggcg ctggagctcg gcgccgacga ttacgtgacc
      901 aaaccgttcg ccctgcacga atttttggcg cgcgtgaagg cgcgactgcg ccggcaggcg
      961 ccgatccaat ccgggcacga cggtgcgatc gcgctgggcg agatcgtgct cgacccgtcg
     1021 cgccagcagc tcaccgttcg cggcaagaac gtggcgctgg caccgaagga gttcgcgctc
     1081 ctgcgcgtgc tgatggagaa caacgggcgc gtcgtgacgc gccagacgct gctcgacaag
     1141 gtgtggggct acgacttcga aggcgagcag cagacggtca gcgtgcacat tcgctggctg
     1201 cgcgagaaga tcgaagaaga ctcgggcacg ccgcggcaca tcctgacggt gcgcagccgc
     1261 ggttacatgt tcaagtccta ggctccctaa accgtgacgt ttccgctgga atgggtgctg
     1321 ccggcggcgg cgatcggggt gctggccggc gttctgctcg
//
LOCUS       DASUCJ010000174         1260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1224465, whole genome shotgun sequence.
ACCESSION   DASUCJ010000174 DASUCJ010000000
VERSION     DASUCJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224465"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFU90_02180"
     CDS             <1..863
                     /locus_tag="VFU90_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5478605.1"
                     /translation="LHNGKYWGRIAALAARVPIVVFTEHSPQGEARSFPEMLVDEVIN
                     RITDAVITFTDQQRSMLRAKERIPSAKLAVIENGIALPDPPDECRRNDARGRLRLEGR
                     EFAVAIVGRLAPVKNTQLGIRALAALPASIRDDVRLCVIGDGSESESLHELARSLGVE
                     ERVTFFGHRGDAKQLLYGADVLFIPSLVEGMPLAGLEAMTVGLPVISTPWPGSRELFS
                     ARGTGLVLADWQPHTAAAALQYVARNPADLRRMGECALRFARTRYDIRRAAREHEQLY
                     AELARRKGLR"
     gene            860..>1260
                     /locus_tag="VFU90_02185"
     CDS             860..>1260
                     /locus_tag="VFU90_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478606.1"
                     /translation="MIADRRRVLLVSYWVPPRTAIGAIRSSYLIKHLPHFGWDVDVLT
                     ARFDEDAVNGARDWTQTGYWDVKGAIKRAAGIGERSTSEVLNLDGFYYGGKPGIAGRA
                     VRLTADLVTYPDEYVGWLPFAVPSLRGKLKGR"
BASE COUNT          228 a          390 c          401 g          241 t
ORIGIN      
        1 acctgcataa cggaaagtat tgggggcgca tcgcagcgct tgcggcgcgc gtgccaatcg
       61 tcgtttttac cgagcacagt ccccagggag aagctcgcag ttttcccgag atgctggtcg
      121 atgaagtgat taaccgcatc acggacgcag tcattacgtt taccgatcag cagcgttcca
      181 tgctgcgcgc caaggagcgt attccttccg caaaactcgc ggtgatcgaa aacggcatcg
      241 cgttgccgga tccaccggac gaatgccgcc gcaacgacgc acggggccgc ctccgtttgg
      301 agggacgcga atttgccgtc gcgatagtcg ggcgtctggc gccggtgaag aacacccagc
      361 tcggcatacg cgcactcgct gcgctgcctg catcaatacg cgatgacgtc cggctgtgcg
      421 ttatcggcga cgggtccgaa tccgaatcgc tgcacgaact cgcgcgatcg ctcggcgtcg
      481 aggagcgcgt cacatttttt ggccatcgcg gcgacgcaaa acagctcctt tacggcgccg
      541 acgttctctt tataccttcg ctcgttgagg gaatgccgct cgccggcctc gaggcgatga
      601 ccgtcggctt accggtgatc agcacgccgt ggccgggatc gcgcgagctc ttcagcgctc
      661 gggggaccgg actggtgctg gcggattggc agccgcacac ggccgcggcg gctctccaat
      721 acgttgcgag aaatccggcc gatctccgcc ggatgggcga gtgcgctttg cgctttgcgc
      781 gaacgcgata tgacattcgg cgcgccgcac gcgaacacga acagctctac gcggagctcg
      841 ctcgccgaaa ggggttgcga tgatcgccga tcgccgccgc gtgctgctcg tgtcgtactg
      901 ggtacctcct aggacggcga tcggcgcgat ccgatcgtcg tacctcatca aacacttgcc
      961 gcacttcggc tgggacgtgg acgttttgac ggcgcgcttc gatgaagacg ccgttaacgg
     1021 cgcgcgcgat tggacgcaga ccggctattg ggacgtcaaa ggcgcgatca aacgcgccgc
     1081 cggtatcggc gagcgaagca cgtccgaggt gctgaatctc gatggttttt attatggagg
     1141 caaacccggt atcgcggggc gtgccgtacg tttaacggcc gatctcgtga cgtatcccga
     1201 cgaatacgtc ggatggctcc cctttgccgt gccctcactg cgcggcaagc tcaaagggcg
//
LOCUS       DASUCJ010000175         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_781233, whole genome shotgun sequence.
ACCESSION   DASUCJ010000175 DASUCJ010000000
VERSION     DASUCJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781233"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFU90_02190"
     CDS             <1..213
                     /locus_tag="VFU90_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5478607.1"
                     /translation="VMIVHEDTRTGGVAGEVTARITERAFAWLDAPILRVTAADVPLP
                     YSPPLEDFVLPQTTDIVTVARQLAAY"
     gene            293..499
                     /locus_tag="VFU90_02195"
     CDS             293..499
                     /locus_tag="VFU90_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478608.1"
                     /translation="MIGVGLAQIVDTVTGCKAGADLPSCNTFTFFLGGGVVGAIVMMV
                     LVQWRIRRRADGAVRTGLKQSKRS"
     gene            504..1868
                     /locus_tag="VFU90_02200"
     CDS             504..1868
                     /locus_tag="VFU90_02200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5478609.1"
                     /translation="MSRIDVIMPQMGESIAEGTLSKWLKQVGDEVKRDEPIFEISTDK
                     VDAEIPAPSAGVLAEILVQEGQTVPVQTVVARLETEKGALVGAGAAPSAGAGAARGAP
                     PAVESTPAAAELSHPSAATPSVPVHAGNGGGNSFEDRLRTKSSPLVRKIAAEHGIELT
                     GMRGSGIAGRVTKKDIMQYLDAGGAAPRPAAPSAPGAPIAPRQAVAASLGTMWPTPIA
                     EPGDVVEPMSKMRALISEHMALSRRTAAHVTSFMEIDFTRVARLRAKHRADFEAATGQ
                     KLTYLPFIIKAVVDGIKAFPVMNASVANDTIIYRKQVNIGVAVALDWGLIVPVIKHAD
                     DLSITGITRTLNDLAGRARAKRLNPEEVQGGTFTITNPGVFGSLMGTPIINQPQVAIL
                     GVGTIEKRPKVMTGPDGEDVIAIRTCSYFSISFDHRIIDGAVADQFMAHVKKTIETYP
                     EPGF"
BASE COUNT          330 a          593 c          658 g          337 t
ORIGIN      
        1 gtgatgatcg tgcacgagga tacgcgcacc gggggcgtgg cgggtgaagt gacggcgcgg
       61 attaccgagc gcgcgttcgc gtggctggat gcgccgattc ttcgcgtgac cgcggcggac
      121 gtgccgctgc cgtattcacc gccgctcgag gatttcgtgt tgccacagac gacggacatc
      181 gtgacggtgg cgcgccagct cgcggcatac tgaggtgggc agggaacgca cgggaccgcc
      241 gcgcctggcg atcggatgtc tgttgttgcc gggaagcttt tttggcggtg gcatgattgg
      301 cgtcggattg gcgcagatcg tcgatactgt gacggggtgc aaagcggggg ccgacctgcc
      361 gtcgtgcaac acgttcacgt tttttctcgg cggcggcgtc gtcggcgcaa tcgtcatgat
      421 ggtcctggtc cagtggcgta ttcgacgtcg tgcagacggc gccgttcgga cgggattgaa
      481 acaatcgaag aggagctgag ccagtgtcac gcatcgatgt gatcatgccg cagatgggcg
      541 agtcgatcgc ggagggaacg ctttcgaagt ggttgaagca ggtgggcgac gaagtgaagc
      601 gcgacgagcc gatcttcgag atctcgaccg acaaggtgga cgcggagatt ccggcgccct
      661 cggcgggggt gttggcggag attttggtgc aggaagggca gacggttccg gtgcagacgg
      721 tggtggcgcg tctggagacg gagaaggggg cgttggttgg ggcgggtgcg gcgccatcgg
      781 ctggtgcggg cgccgcccgg ggggcccccc ccgctgtcga atctacgccc gcggcggccg
      841 agttgtctca tccttctgcg gcgaccccaa gcgttccagt gcatgccggc aacggcggcg
      901 ggaattcgtt cgaggaccgc ctgcgcacga agtcatcgcc gctcgtgcgc aagatcgccg
      961 cggagcacgg catcgagctc accggcatgc gcggttcggg catcgccggc cgcgtgacga
     1021 agaaagacat catgcaatac ctcgatgccg gcggcgccgc gccgcgtccc gccgcaccgt
     1081 cggcccccgg cgcaccgatc gcgccgcgcc aggccgtcgc cgcgtcgtta ggcaccatgt
     1141 ggcccacgcc catcgccgaa cccggtgatg tcgtcgagcc catgtccaag atgcgtgcgc
     1201 tcatcagcga gcacatggcc ctgtcgcgcc gcaccgccgc ccacgtgacg tcgttcatgg
     1261 agatcgactt cacgcgcgtc gcccgcttgc gcgccaagca ccgcgcggac ttcgaggccg
     1321 ccaccggtca gaagctgacg tatctgccgt tcatcatcaa agccgtcgtc gatggcatca
     1381 aagccttccc ggtgatgaac gcctcggtcg cgaacgacac gatcatctat cgcaagcaag
     1441 tgaacatcgg cgtcgccgtc gcgctcgact ggggactcat cgtcccggtc atcaagcacg
     1501 cggacgacct ttcgattacc ggcatcacgc gcacgctcaa cgaccttgcc ggacgtgcgc
     1561 gcgccaagag gctgaatccg gaagaggtgc agggcgggac gttcacgatc acgaatcccg
     1621 gcgtgttcgg ctcgctcatg ggaacgccga tcatcaatca gccgcaggtc gcgatcctcg
     1681 gtgtgggcac gatcgagaag cggccgaaag tgatgaccgg tccggacggt gaagatgtga
     1741 tcgcgattcg tacctgttcg tacttctcga tctcgttcga ccaccgcatc atcgacggcg
     1801 ccgttgccga tcagttcatg gcgcacgtca agaaaaccat cgaaacgtat cccgagccgg
     1861 gattctgacc gtggccagag cgctcaccat gcggcgttcg atcgttcctc tcgccgat
//
LOCUS       DASUCJ010000176         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1316040, whole genome shotgun sequence.
ACCESSION   DASUCJ010000176 DASUCJ010000000
VERSION     DASUCJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316040"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFU90_02205"
     CDS             complement(<1..1027)
                     /locus_tag="VFU90_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEU5478610.1"
                     /translation="MLAGMSTHPATAHTSPLKLRTPVDIVDAVPHLVGFQPENSLVCM
                     SLRGPRKRLGVVSRADLPPVRFARPFATQTARYLKHDGAEHVILVFYPPDDGPKNGRL
                     IALITALEKALDKQRINVIEVLCVYDGRWWSLLCDDDDCCPPDGTAIDQQRTSLVAAE
                     MAASGRVVFKSRAELERTLEPVRGAAARDMRAELSRARSDVHGRTAAGGAAEVAAESI
                     KLYEDAVRRRQQVAETMTSIGSDDAARLIVALDDVAVRDQILTWAEGDRGEALQRLLA
                     ELAPRALEGHEAPVLTAYALTCYVQGEGALAGIALDRARQADPTYNLARILDDALLGC
                     VNPAVLRD"
BASE COUNT          235 a          433 c          391 g          216 t
ORIGIN      
        1 aatcgcgcag caccgccgga ttcacacaac caagcagcgc gtcatcaagg atccgcgcca
       61 ggttgtacgt cggatcggct tggcgggcgc gatcgagagc gatgccagcg agtgcgccct
      121 cgccttgcac gtagcaggtc agcgcgtaag ccgtcagaac aggtgcttcg tgaccctcaa
      181 gcgcccgcgg cgccaactcc gccaacagcc gttgcaacgc ctccccgcga tcgccctcgg
      241 cccaggtcag aatttgatcg cgcaccgcca cgtcgtcgag cgcgacgatg agccgcgcag
      301 cgtcgtccga accgatcgac gtcattgtct cggcgacctg ctgccgtcgg cgaaccgcgt
      361 cttcgtaaag cttgatcgat tcggccgcga cctcggccgc accgcccgcc gcggttcgac
      421 cgtgcacatc ggatcgcgct cgcgacaact cggcgcgcat gtcacgcgcc gccgcgcccc
      481 gaaccggctc cagtgttcgc tccaactcgg cgcgtgactt gaacaccacg cgcccgctcg
      541 ccgccatttc agcggccact aaagaagtgc gttgctggtc gatcgcggtg ccgtcaggcg
      601 ggcagcagtc atcgtcgtcg caaagcagcg accaccatcg cccgtcgtag acgcacagca
      661 cctcgatcac gttgatccgt tgcttgtcga gcgccttctc aagggcggtg atcagcgcga
      721 ttagccggcc gttcttcggg ccgtcgtcag gcggatagaa gacgaggatg acgtgttccg
      781 caccgtcgtg cttgaggtag cgagcggtct gcgtcgcgaa cggccgagca aagcgaaccg
      841 gcggcagatc cgcccgactc accactccaa gccgcttacg cggaccgcgc aaagacatac
      901 agaccagcga gttttcgggc tgaaagccga cgagatgcgg caccgcatcg acgatgtcga
      961 ccggtgtgcg gagcttgagc ggcgacgtgt gtgcggtcgc aggatgcgtt gacatgcctg
     1021 cgagcatgac ggttttgcag ggccaacagc gctaggccac tcgcgacctg tggatagcga
     1081 tcaagcgcgc tgtgagctca gccccagact tccgcagccg tctcgacgat gagccgcagc
     1141 ttggcgacct cttcctcgta cgtgagtgag tttccctcaa cagttgagga aaatccgcac
     1201 tgcggcgaca agcacagctg ttcgagcggc acgaacttgg ccgcttcgtc gatacgccgc
     1261 ttcaagtcgt ccttg
//
LOCUS       DASUCJ010000177         8489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1361975, whole genome shotgun sequence.
ACCESSION   DASUCJ010000177 DASUCJ010000000
VERSION     DASUCJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361975"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="wecB"
                     /locus_tag="VFU90_02210"
     CDS             complement(<1..514)
                     /gene="wecB"
                     /locus_tag="VFU90_02210"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5478611.1"
                     /translation="METRLKVMTVFGTRPDTIKMAPVVRALAERPEIDDVVCVTAQHR
                     QMLDDLLAAFSIEPDYDLDIMTEDQTLTEITTRVLERMEPVLRDARPDVVLVHGDTTT
                     STSAALAAFYQQIDVGHVEAGLRTNDKWLPYPEEMNRRLTGTIATYHFSPTPLARDHL
                     LRENVPAADVI"
     gene            complement(544..1632)
                     /locus_tag="VFU90_02215"
     CDS             complement(544..1632)
                     /locus_tag="VFU90_02215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU5478612.1"
                     /translation="MSGPGWSAAAIYFATFAIAALVTSVATPLIVRLATHLGVLDHAE
                     EERRVHDVPTPRIGGIAVFFGFACALFAVLGFALQSPFALLPSAVHESHAKQIELLTD
                     QFSTVHQLVGLLFGSLLILGVGIWDDVIGMQPRNKFVAQVLVALISMFYGFMITGATN
                     PFNHDPNTNWIEFPLWLGVPLTLLWYVGMMNAINFIDGLDGLLAGVAAISSVFIFAIS
                     VLHANPVVALVVIALAGAALGFLPYNFNPARIFLGDAGSLFIGYVFATVSIIGASKQA
                     IAISVFVPLLALALPVLDTAAAIVRRAIAGKRITEADLGHFHHQLIFRFGLNVRQAVL
                     LIYAVCFVLGAMALALSGEFTHVFRHAS"
     gene            complement(1629..2066)
                     /locus_tag="VFU90_02220"
     CDS             complement(1629..2066)
                     /locus_tag="VFU90_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU5478613.1"
                     /translation="MRPGWDEYFMQIAYTVSSRSTCPRASVGCALVREHRILTTGYNG
                     APRGVAHCTEVGCTMAGGHCVRATHAEANAVVQGALHGVSLDQATAYCTHQPCVNCSK
                     LLISAGVRRIVYDGSYPDDVAVALLAEAGVALVRYGSLEGARG"
     gene            complement(2082..3329)
                     /gene="glyA"
                     /locus_tag="VFU90_02225"
     CDS             complement(2082..3329)
                     /gene="glyA"
                     /locus_tag="VFU90_02225"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5478614.1"
                     /translation="MDILTPSSIETEDPEVFAAIAGEERRQRDNLELIASENYASRAV
                     REAMGCVMTNKYAEGYPGKRYYGGCEYVDQAELLAIDRLRRLFSAEHVNVQPHSGAQA
                     NMAVFMAHLQPGDTVLGMSLAHGGHLTHGTKVSFSGKLYNAVAYGVDRTTERIDFDEV
                     RALAREHKPKLIVAGASAYPRIMEYGPFREIADEVGAALLVDMAHVAGLVAVGLHPSP
                     VAIADFSTSTTHKTLRGPRGGIILCLQRWAQAVDKSVFPGIQGGPLMHTIAAKAVAFG
                     EALRPDFALYQRQVIDNARAMADEFTAAGLRLVAGGTDTHLLLVDVSVKGLTGKATEA
                     YLDEIGITVNKNAIPFDSQKPMVASGIRIGTPAITTRGFGLDECREVARIMIDGLDDI
                     ETRAQIERLRGRVRELTARFGVP"
     gene            complement(3345..3863)
                     /gene="rpiB"
                     /locus_tag="VFU90_02230"
     CDS             complement(3345..3863)
                     /gene="rpiB"
                     /locus_tag="VFU90_02230"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU5478615.1"
                     /translation="MKIALGADHAGFEYKDRIAGLLRAGGHEVLDFGTHDATPVDYPQ
                     YGYAVGEAVATGQADRGIVVCGSSLGIAMAANKVPGIRCAPVAEPYSAELARRHNDCN
                     VIAFSERLTGWEMIERMLEIFLETPFDGGRHAYRVEQLFEFGDRQRERTLVDLERGAV
                     TDAQTPQDLLPK"
     gene            3938..4657
                     /locus_tag="VFU90_02235"
     CDS             3938..4657
                     /locus_tag="VFU90_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU5478616.1"
                     /translation="MKLACASGALHHSIVSGDLTQLEFIDLCAHELACDGVVFDVRHF
                     PRTDDDYLAQVKKMAADRGLTVAAVTDSAFFAAPDAAMAGSLRLAVALGAPLVAGTLA
                     RELDLAWSDQLARLNAATALAKACNVTLAVRNAPGTFAASTHDCKRVAKEADSAWLRF
                     GPEPRAFDAASDPRDLAANAVLLWNGIGEQTERSIDATIADFAAFRGHVTLDEASGDA
                     RTADLLAAMRSWRIALAAAAR"
     gene            5190..5777
                     /locus_tag="VFU90_02240"
     CDS             5190..5777
                     /locus_tag="VFU90_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478617.1"
                     /translation="MQPLTLAPGAGYVRHGSLSVPRRAERAYAEAKAYLTRDRIERSL
                     FDRLEHARGRRFRLTINHRNDDHFDPRTDTIAWDPHSALRTTGGGRQSPALGLGHEID
                     HAVESPARERRLALRPCRHYDDAEEKRVITGSERHAARTLGEGVRYDHSGTTYWVAAP
                     EATRPRNALQRVECGLDARIDGLRHPARASARPPV"
     gene            5719..6738
                     /locus_tag="VFU90_02245"
     CDS             5719..6738
                     /locus_tag="VFU90_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU5478618.1"
                     /translation="MPALTVSVTPLELPLVHPFKIARGEETVARTALVRVRFGESEGL
                     GEAAPVERYGESVESVADYFATHPLAESNPFRLESLLHDAIPPAARSGLDLALHDLVG
                     KELGKPLYELFGLDPSLTPVTSFTIGIADLETTLRKVDEVGDHPILKVKLGSGPPGRQ
                     IETVAAIRERYRGTIRLDANEGWDVETAVAILRELERFEIEFCEQPVPAGHPEQLGAV
                     RERVKVPIVTDEDSCVAADLPALAGCVDGVNVKLAKTGGLRGALAMIHTARALKMKVM
                     LGCMVESAICATAAAHISPLVDWADIDGPFLTARDPFAGVTYDAGKLVLPNAPGIGIR
                     QTVPA"
     gene            6735..7805
                     /locus_tag="VFU90_02250"
     CDS             6735..7805
                     /locus_tag="VFU90_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEU5478619.1"
                     /translation="MSRRYLILAPGRFAIDAKTAHGVIRYGDDPVVAVVDPACAGKRV
                     RDVLAHLNSDAPIVASVAEGLQFEPTALLIGTAPRGGALPPDWRRAVLEAIAARLEIV
                     SGLHDMLDDDLEFRAAAMRARTVIWDVRKPPDVPLFGGAVYDVEAPVLLTVGNDCAVG
                     KMSVALEIVRAARAAGRPGARFVPTGQTGIMIAGWGISVDRVVADFAPGAAEQLVLYA
                     AREGGDPIVVEGQGAINHPAYAPVTLALMYGSAPDALILVCDPSRTRIEDYPTPVLGY
                     RELVAIHEGLLATVKPAKVAGIALNTRGLTDERAHTEIERARRESGLPADDVVRFGAS
                     DFYAAIAPQVVKRAPLRAGDSA"
     gene            7802..>8489
                     /locus_tag="VFU90_02255"
     CDS             7802..>8489
                     /locus_tag="VFU90_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5478620.1"
                     /translation="MSRALPRLAAAALALAVATGCAKSGVRPANGKNPFTIPGVLRLG
                     EAEEPDSLNLMYAHTAAADEISGLLFSFLLRYDRDGNYVPDLATAVPSPGNGGISRDG
                     KTIVVHLRKGVTWADGAPLTAADWLFTYRAAIDPRNNVKTRYGWDDIASARASDPYTL
                     VIRLKRPTVTVLGILAMGGAGYPPMPAHVLAKLPDLNSADFNSRPLSSGPYLLKAWNR
                     GSSLEFVPNPR"
BASE COUNT         1430 a         2852 c         2810 g         1397 t
ORIGIN      
        1 cgatgacgtc cgcggcggga acgttctcgc gcagcagatg atcgcgggcg agcggcgtcg
       61 gcgagaaatg ataggtcgca atcgttccgg tgagccgccg gttcatctcc tcggggtagg
      121 gcagccactt gtcgttcgtg cgcagccccg cctctacgtg gcctacgtcg atctgctggt
      181 aaaacgccgc cagcgcggcc gacgtgctgg tggtcgtatc gccgtgcact aagacgacgt
      241 cggggcgcgc gtcgcggagc accggttcca ttcgctcgag cacgcgcgtg gtgatctcgg
      301 tgagggtctg atcctcggtc atgatgtcga gatcgtagtc cggttcgatc gaaaaggcgg
      361 ccagcagatc gtccagcatc tgccggtgct gggcggtcac gcagacgacg tcgtcgattt
      421 cgggacgctc ggccagcgcg cgcacgaccg gcgccatctt gatggtgtcg ggacgcgtcc
      481 cgaagaccgt catcactttc agacgtgttt ccaaacaaac ctcgatgtaa tggtgccgct
      541 cccttaggaa gcgtgcctga agacgtgcgt gaattcgccc gagagcgcga gcgccatcgc
      601 gccgaggacg aaacataccg cgtagatcaa cagcacggcc tggcggacgt tcagcccgaa
      661 ccggaagatc agctggtggt ggaagtgtcc caaatccgcc tcggtgatcc gcttcccggc
      721 gatcgcccgg cgcacgatcg cggccgccgt atcgagcacc ggtagcgcca gcgccagcag
      781 cggcacgaaa acgctgatcg caatcgcctg cttgctcgcg ccgatgatcg agaccgtcgc
      841 aaagacgtag ccgatgaaga gcgatccggc gtcgcccaaa aagatccgtg ccggattgaa
      901 attatacgga aggaaaccga gcgccgcccc ggcaagcgcg atcaccacga gcgcgaccac
      961 cgggttggcg tgcagcaccg agatggcgaa aatgaagacg ctcgagatcg ccgcgacgcc
     1021 ggccagcaat ccgtcgaggc cgtcgatgaa gttaatcgcg ttcatcatcc cgacgtacca
     1081 aaggagcgtg agcgggaccc cgagccagag cgggaattcg atccagttgg tgttcgggtc
     1141 gtggttgaac ggattggtgg ccccggtgat catgaagccg taaaacatcg agatcagcgc
     1201 caccaggact tgggccacga atttattgcg aggctgcatg ccgatgacgt cgtcccaaat
     1261 gccgaccccg aggatcagca ggctgccgaa gagcagcccg accagttggt gcacggtcga
     1321 aaactgatcg gtaagcagtt cgatttgctt ggcgtgcgat tcgtgcacgg ccgacggcag
     1381 cagcgcaaac ggcgattgaa gcgcgaagcc gagcacggcg aacagcgcgc acgcgaaacc
     1441 gaagaatacc gcaattccgc cgattcgcgg cgtcggcacg tcgtgcacgc ggcgctcttc
     1501 ctcggcgtgg tcgagcacgc cgagatgggt cgccagccgc acgatcagcg gcgtcgccac
     1561 cgacgtcacc agcgcggcga tcgcgaaggt cgcgaagtag atcgccgcgg cactccagcc
     1621 cgggccgctc atccgcgggc accctccagc gagccgtacc gaacgagcgc gacgccggct
     1681 tcggccagca gcgcgacggc gacgtcgtcg gggtagctgc cgtcgtagac gatccggcgg
     1741 acgcccgcgc tgattagcag cttcgagcag ttcacgcagg gctgatgcgt gcagtacgcg
     1801 gtcgcctggt cgaggctgac gccgtggagc gctccttgca cgaccgcgtt ggcttcggcg
     1861 tgggtcgcgc gcacgcaatg accgccggcc atcgtgcagc cgacctccgt gcagtgggct
     1921 acgccgcgcg gcgcgccgtt ataccccgtc gtcagaatgc ggtgctcgcg cacgagcgcg
     1981 cagccgaccg aggcgcgcgg acacgtcgag cgggagctca ccgtgtacgc aatctgcatg
     2041 aagtattcgt cccatcccgg ccgcacgtcg actccaagtt gctacggcac gccgaaacgc
     2101 gccgtcagct cgcgaacgcg gccccgcagc cgttcgatct gcgcgcgcgt ctcgatgtcg
     2161 tcgaggccgt cgatcatgat ccgcgcgacc tcgcggcatt cgtccagacc gaaaccgcgg
     2221 gtcgtgatcg ccggcgtacc gattcgaatg ccgctggcga ccatcggctt ctgggaatcg
     2281 aacggaattg cgtttttgtt caccgtgatg ccgatctcgt cgaggtaggc ctccgtcgcc
     2341 ttccccgtca gccccttgac cgacacgtca acgagcagta agtgggtatc ggtgccgccg
     2401 gcgaccagcc gcaaaccggc tgcggtgaat tcgtccgcca tcgcgcgcgc gttatcgatc
     2461 acttggcgct gatagagcgc gaagtccggg cgcagggcct cgccgaaggc caccgcttta
     2521 gcggcgatcg tgtgcatcag cggaccgcct tgaatgcccg ggaagacgct cttgtcgacg
     2581 gcctgcgccc atcgctgcaa gcagaggatg attccgcctc gcggcccgcg cagggtctta
     2641 tgggtcgtcg acgtcgaaaa atcggcaatc gctaccggcg aaggatgcag cccgaccgcg
     2701 accagcccgg cgacgtgcgc catgtcgacc agcagcgcgg cgccgacctc gtcggcgatt
     2761 tcccggaagg gcccgtactc catgatgcgc gggtaggcgc tggcaccggc gacgatcagc
     2821 ttcggcttgt gctcgcgcgc cagcgcgcga acttcgtcga agtcgatccg ctcggtcgtt
     2881 cgatcgacgc cgtaggcgac cgcgttgtaa agcttgccgg agaagctgac tttggtgccg
     2941 tgggtgagat gcccgccatg cgccagcgac atgcccagaa cggtatcgcc gggctgcagg
     3001 tgcgccatga agaccgccat gttcgcctgc gcgccggaat gcggctgaac gttgacgtgt
     3061 tcggccgaga acagccgtcg gaggcggtcg atcgcgagca gctcggcttg gtcgacgtat
     3121 tcgcatccgc cgtagtagcg cttaccggga tatccttccg cgtacttgtt ggtcatcacg
     3181 cagcccatcg cttcgcgaac cgcacggctg gcgtagtttt ccgaagcgat cagctcgagg
     3241 ttgtctcgct gccggcgctc ctcgccggct atggcggcga agacttccgg gtcttcggtt
     3301 tcgatgctgc tgggcgtgag gatgtccatg atcgttcgtc ccgtttactt cggaagcagg
     3361 tcctgcggcg tttgcgcgtc ggtcaccgcg ccgcgctcga gatcgacgag cgtgcgctcg
     3421 cgctgccggt cgccgaattc gaacaactgc tcgacgcggt acgcgtgccg cccgccgtcg
     3481 aacggcgttt ccaagaatat ctcgagcatc cgttcgatca tttcccagcc ggtcagacgc
     3541 tccgagaacg cgatcacgtt gcagtcgtta tggcggcgtg cgagctcggc cgaatacggc
     3601 tcggccaccg gcgcgcaccg aattccggga accttgttgg cggccatcgc gatgccgagg
     3661 ctcgagccgc agacgacgat gccgcgatcg gcttgaccgg tcgcaaccgc ttcgcccacc
     3721 gcgtagccgt actgcggata gtccaccggg gtggcgtcgt gcgtgccgaa atcgagcact
     3781 tcgtgccccc cggcgcgcaa cagcccggcg atgcgatcct tgtattcaaa tccggcgtga
     3841 tcggcgccga gggcgatttt catgtttctc cagcgatcgt ccggcgatac cgtgacgagc
     3901 aaggggggcg ttcggagcag tcgaacgacg aacgccgatg aagttggctt gcgcgagcgg
     3961 agcgctccac cactcgatcg tctccggcga cctcacccaa ctcgagttta tcgacctctg
     4021 cgcgcacgag cttgcttgcg acggcgtcgt atttgacgtg cggcactttc cgcggaccga
     4081 cgacgactac ctcgcgcagg tcaagaagat ggcggcggac cgggggctca cggtggcggc
     4141 ggtgacggac tcggcattct tcgccgcgcc ggacgcggcg atggcgggtt cgctccggct
     4201 cgccgtcgcg ctgggtgcgc cgctggttgc gggcacgctg gcacgggagc tcgaccttgc
     4261 gtggagcgat cagctcgcgc gactgaacgc cgcaacggcg ctggcaaagg cgtgcaacgt
     4321 cacccttgcc gttcgcaacg cgcccggaac gtttgccgct tcgacgcacg actgcaagcg
     4381 cgtcgccaag gaggcggatt cggcgtggct gcgtttcggg ccggagccgc gcgcgttcga
     4441 cgctgccagc gatccgcgcg acctggcggc caacgcggtc ttgctctgga acggcatcgg
     4501 cgagcaaacc gaacgctcga tcgatgcaac gatcgccgat ttcgcggcat ttcgcgggca
     4561 cgtaacgctc gacgaggcgt cgggcgacgc gcgcacagcc gatctgctcg cagcgatgcg
     4621 gtcgtggcgc attgccctcg ccgcagccgc acgatgaatc gcccaaccgc acgtagcgtc
     4681 tgccgcactc ggtaatcgaa cctgcgtctc gcaggtgggt gcggcccggg cgccagccgg
     4741 caggcattcg attcccgcca gtccgtcgta tccggagctg atgctcccta ggtgtcgtat
     4801 atcggctcgg cacctaagcg cggtgcgcgc tacgcgcagt cgagcctctc taccgtacca
     4861 ccggggtcaa gggcttgatt ttcctccggc ggcgtgctag cggtttgcga tcatggcacg
     4921 gcgcatccga gcttgcttgg aaggcagagc cgactcgtcc gcgacctacc tgccggtgcc
     4981 ggcgtccgcc gtgcgggcgt tcggcgggcg cacgcgcgtt cgacgcaacc gcgttttcgc
     5041 accgcaaaga atacgtcgcc tggatcgagg acgcgaagaa accggagacg cggcttcgcc
     5101 ggattcaaaa agcctgcgag cggctccgcg cgcgcgccgc atccaagact cctagacgta
     5161 cgccgattcc ggcgtgatgg ctgcgggcca tgcagccgct cacgctcgcg cccggcgcgg
     5221 gctacgtccg tcacggttcg ctttcggtgc cgcgccgcgc cgaacgcgcg tacgccgaag
     5281 ccaaagccta tctcacgcgc gatcggatcg agcgatcgct gttcgaccgc ctcgagcacg
     5341 cgcgcggacg gcgctttcgc ttgacaatca accatcgcaa cgacgaccac ttcgatccgc
     5401 gtaccgacac gattgcgtgg gatccgcact cggcgctgcg aacgaccggc ggcggccgcc
     5461 agtcgcccgc gcttggactc ggccacgaaa tcgaccacgc cgtcgagtca cccgcgcgcg
     5521 aacgccggct cgcactacgc ccgtgccggc attacgacga cgccgaggag aagcgcgtca
     5581 tcaccggatc cgaacgtcac gccgcccgca cgctcggcga aggcgtgcgt tacgatcact
     5641 cgggcacgac gtattgggta gccgcgcccg aggcgacgcg cccgaggaac gctctgcagc
     5701 gggtggaatg cggtttggat gcccgcattg acggtctccg tcaccccgct cgagcttccg
     5761 ctcgtccacc cgtttaaaat cgcgcgcggc gaagaaaccg tagcgcgtac cgcgctcgtg
     5821 cgggtgcgtt tcggcgagag cgaaggcctc ggtgaggccg cgccggtcga acgctacggc
     5881 gaatcggtgg aaagcgtcgc ggattatttc gcaacgcacc cgctcgccga atcgaatccc
     5941 tttcgtctcg aaagcctgct gcacgacgcg attccgccgg cggcgcgatc cggactcgat
     6001 ctcgcgctgc acgatctcgt cggcaaggag ctggggaaac cgctctacga gctcttcggc
     6061 ctcgacccga gcctgacgcc ggtaacctcg ttcacgatcg gaatcgcgga tctcgaaacg
     6121 acgctgcgca aagtcgacga ggtgggcgat cacccgattc tcaaagtgaa actcggcagc
     6181 ggtccgcccg gccggcaaat cgaaacggtc gcggccatcc gggagcggta ccggggtacg
     6241 attcggctcg atgcaaacga aggctgggac gtcgaaaccg cggtggcgat cctgcgcgag
     6301 ctcgaacgct tcgagatcga gttttgcgaa cagccggtgc cggcgggtca tcccgagcag
     6361 ctgggcgcgg ttcgcgaacg tgtgaaggtt ccgatcgtga ccgatgagga ttcgtgcgtc
     6421 gctgccgacc tgccggccct ggccggctgc gtcgacggcg tgaacgtgaa actcgcgaaa
     6481 accggcggtc tgcgcggcgc gctcgcgatg atccacaccg ctcgggcgct gaagatgaag
     6541 gtcatgctcg gctgcatggt agagagcgcg atttgcgcga cggccgcagc gcatatttcg
     6601 ccgctggtcg attgggccga catcgacggg ccgtttctca ccgcgcgcga tccgtttgcc
     6661 ggagtcacct acgacgccgg caagctcgtt ctcccgaacg cccccggcat cggcatacgg
     6721 caaaccgttc ccgcgtgagc cgccgctatc tgatcctcgc tcccgggcgc ttcgcgatcg
     6781 acgcgaaaac ggcgcacggc gtcatccgct acggcgacga tccggtggtc gccgtggtcg
     6841 atccggcctg cgccggaaaa cgcgtgcgcg acgtgctcgc gcatctgaat agcgacgcgc
     6901 cgatcgtcgc gagcgtcgcc gaagggctgc agttcgagcc gacggcgctc ttaatcggaa
     6961 ctgcgccccg cggcggcgcg ctcccgcccg attggcgccg cgcggtgctc gaggcaatcg
     7021 ccgcacgcct ggaaatcgtc agcgggctgc acgacatgct cgacgacgat ctcgagtttc
     7081 gcgccgccgc gatgcgcgcg cgtacggtta tttgggacgt gcgtaaaccg cccgacgttc
     7141 cgctcttcgg cggcgcggtc tacgacgtcg aagcgcccgt gctcctgacc gtcggcaacg
     7201 actgcgcggt cggaaagatg agcgtcgcgc tggaaatcgt gcgcgcggcg cgcgccgccg
     7261 gccgccccgg cgcgcgcttc gtgccgaccg ggcaaaccgg aatcatgatc gccgggtggg
     7321 gaatctccgt cgaccgcgtc gtcgccgact tcgcgccggg cgccgccgaa cagctcgtgc
     7381 tctacgccgc ccgcgagggc ggcgatccga tcgtcgtcga gggtcagggg gcgatcaatc
     7441 acccggctta cgccccggtg acgctggcgc tgatgtacgg aagcgctccc gacgccctca
     7501 tcttggtgtg cgatccgtcg cgcacgcgca tcgaagatta tccgaccccg gtgctgggtt
     7561 accgcgagct cgtggcgatt cacgaagggc tgctcgcaac cgtcaagccg gccaaggtcg
     7621 ccggcatcgc gctcaacacg cgcgggctga ccgacgagcg cgcgcatacc gaaatcgaac
     7681 gtgcgcggcg cgaaagcggt ttgccggccg acgacgtcgt ccgcttcggt gcgagcgact
     7741 tttacgccgc tatcgcgccg caggtggtca agcgcgcgcc gctgcgcgcc ggcgactcgg
     7801 cgtgagccgc gcgctgccgc gtttggcggc ggcggcgctc gcgctggccg tggcgaccgg
     7861 ctgcgcgaag agcggcgtgc gccccgcgaa cggcaagaat ccgtttacga tccccggcgt
     7921 cctccggctc ggcgaagccg aggagcccga cagcctcaac ctgatgtacg cgcacactgc
     7981 ggccgcggac gaaatttccg gcttgctgtt ttccttctta ctgcgttacg accgtgacgg
     8041 caactacgtt ccggatctgg cgaccgccgt gccgtcgccg ggcaacggcg gcatcagccg
     8101 cgacgggaag acgatcgtcg tgcacctacg caagggcgtg acgtgggcgg acggcgcacc
     8161 gctgacggcc gccgattggc tattcaccta ccgcgccgcg atcgacccgc gcaacaacgt
     8221 gaaaacgcga tacggctggg acgacatcgc atcggcgcgc gcatccgatc cgtacacgct
     8281 ggtgatccgg ctcaaacggc cgaccgtgac ggttctcggg atcctcgcaa tgggcggcgc
     8341 cggctacccg ccgatgccgg cgcacgtgct cgccaaactg ccggatctca actcggcgga
     8401 tttcaacagc cgcccgctct cgagcggtcc gtacctgctc aaagcctgga atcgcggatc
     8461 gtcgctcgaa ttcgttccga acccgcgct
//
LOCUS       DASUCJ010000178         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_108792, whole genome shotgun sequence.
ACCESSION   DASUCJ010000178 DASUCJ010000000
VERSION     DASUCJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108792"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU90_02260"
     CDS             <1..723
                     /locus_tag="VFU90_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5478621.1"
                     /translation="PYSARAGPTMQRLQAMGFAAALLAGLSAPGIASAESIAGTILYA
                     GPPVAPRKIDVTIDQYVCGHDKDATDLLVSPRRELRNAVVWIDNPPADRASPVPGDTV
                     ELDQQGCVFVPRVVVVQAGGTVNFLNGDRLLHNLHATPKLNVSFNRTQPKGRTIPVVF
                     SKPEIVRINCDLHSWMRAWVVVAAHRYYAVTGADGRFTFDGLPPGNYTLTVWHEDLGT
                     MQANVTVTADRPARITVEMKAR"
     gene            complement(786..1286)
                     /locus_tag="VFU90_02265"
     CDS             complement(786..1286)
                     /locus_tag="VFU90_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEU5478622.1"
                     /translation="MTHFTDRCACAGIHASAGTRMASLATSRIASAAVLVAACAAIPL
                     AHAAGEQVEQFTPEFRQAHTTYDAKHTPKIIAPDSVKRGEWFNVTISVGAGGAHPSLQ
                     EHFVRYIALYKDSVEIARTYLHPVYSAPIVTFTVALDEGGSLRAIEEPTHSAAWEASR
                     KIEVLP"
     gene            complement(1299..>1481)
                     /locus_tag="VFU90_02270"
     CDS             complement(1299..>1481)
                     /locus_tag="VFU90_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478623.1"
                     /translation="ILKANPTTSTIPIVVITSFALSGDREKAAAAGADSYLAKPYSPR
                     ELLALVRRFLPDEGST"
BASE COUNT          250 a          484 c          481 g          266 t
ORIGIN      
        1 ccgtactccg cgcgagccgg accgacgatg caacgattgc aagcgatggg tttcgcggcc
       61 gcattgctgg ctgggctgtc cgccccgggg atcgcttcgg ccgagtcgat cgccggaacg
      121 atcctctacg ccggtccgcc cgtcgcgccg aggaaaatcg acgtcacgat cgatcagtac
      181 gtctgcggcc acgacaagga tgccaccgac ctcctcgtgt cgccgcgccg ggagctcagg
      241 aacgccgtcg tctggatcga caatccgccg gcggaccgcg cgtcacctgt acctggcgac
      301 acggtcgagc tcgatcaaca gggctgcgtg ttcgttccgc gcgtggtggt cgtgcaggct
      361 ggcggcaccg tgaatttcct caacggcgac cggctcctgc acaacctcca cgcgacgccg
      421 aagctcaacg tctcgttcaa tcgcacgcag cccaagggcc gaaccattcc ggtcgtcttc
      481 tcgaagcccg agattgtccg catcaactgc gacctccatt catggatgcg cgcgtgggtc
      541 gttgtcgctg cacatcgtta ctacgcggtg accggtgccg acggccggtt cacgttcgac
      601 ggcctgccgc ctggcaatta cacgctcacc gtgtggcacg aggaccttgg cacgatgcag
      661 gcgaacgtga ccgtcaccgc cgatcggccc gcgcgtatca ccgtcgagat gaaggcgcgc
      721 tgaacgccgt cgccgctggc gagaccggcg cgcgttgacg ctatcgatgc gggcgcgaac
      781 ggagcctacg ggagaacctc gatcttccgg ctcgcttccc aggctgccga atgcgtcggc
      841 tcctcgatcg cgcgcagcga gccgccctcg tcgagcgcca ccgtgaacgt gacgatcggc
      901 gccgagtaca ccggatgcag gtacgtacgg gcgatttcca ccgagtcctt gtatagcgcg
      961 atgtagcgaa cgaaatgctc ctgcagggac gggtgcgcgc caccggcgcc gacgctgatg
     1021 gtcacgttga accattcgcc ccgcttgact gaatcggggg caatgatctt cggcgtgtgc
     1081 ttggcatcgt aagtggtatg tgcctgccgg aactccggtg tgaactgctc gacttgctcc
     1141 ccggcggcgt gtgcgagcgg aattgccgca caagccgcga cgagcacggc ggccgaagca
     1201 atgcgacttg tcgcgagcga ggccatgcgg gtaccggcgc tggcatggat tccggcgcaa
     1261 gcgcaacgat cggtgaagtg ggtcatcgcg tcgtctcctt acgttgatcc ttcgtccggc
     1321 aggaagcgcc ggaccagtgc cagcaattcg cgcggactgt agggcttggc gagatagctg
     1381 tcggccccgg cggccgcggc tttttcccgg tcgccgctca atgcaaacga cgtgatcacg
     1441 acgatcggaa tcgtgctggt tgtcggatta gccttgagga t
//
LOCUS       DASUCJ010000179         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_918837, whole genome shotgun sequence.
ACCESSION   DASUCJ010000179 DASUCJ010000000
VERSION     DASUCJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918837"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..869
                     /locus_tag="VFU90_02275"
     CDS             546..869
                     /locus_tag="VFU90_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU5478624.1"
                     /translation="MIVVTTENVAGYRVAQTLGQVFGVVVRSRGLGGNIVAGLRSIVG
                     GEITEYTEMLEEARRHAVDRMVQNATAMGADAIIMMRFDSSELGQTMSEIVAYGTAVT
                     LTAAS"
     gene            869..1177
                     /locus_tag="VFU90_02280"
     CDS             869..1177
                     /locus_tag="VFU90_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478625.1"
                     /translation="MLRVIVIFFGAALLAVSAAIAIAAPGAWPAAVDCAVLGILILVG
                     TLLEKRYRSARPATGADWQTTGERFMDPSTGQFTQVRYNRQTGERSYEPSDAESFPKI
                     "
     gene            complement(1191..>1579)
                     /locus_tag="VFU90_02285"
     CDS             complement(1191..>1579)
                     /locus_tag="VFU90_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478626.1"
                     /translation="LADASFEVRSRSESQVWSSKTLADVAIWQAALGNLPAARDAVQR
                     LLANDDAIQRGTDWPSYCYWAAAQVQRRDGRSAEAARTLARAERAMQASADALEPEDR
                     ERYLAIPWHADLRRAVAAGAWPDPPR"
BASE COUNT          272 a          516 c          487 g          304 t
ORIGIN      
        1 cgaactcgcg tagcatcgca gccatattaa gcgatcttaa aagagagagg gggacccccg
       61 ctggtccccc tctactgccg ttctaccttc gtctgttacc ccttaagggg cgcccccatc
      121 gtacgttgcc ggtgccggga agaccaggca cagccggcag ttcaccggcg gagatgcact
      181 gtttgggcga ctctcacact acggtcgctg ctactgtgta tctcctccgc cgaaagacct
      241 agtgtacctg gtcattttgc cgccgcggac gtacgatttg gtcccttccc ttacggggag
      301 ctgcagaggg ggaccggagc gggccggtcc ccttcctttt ttatatgcgc cggctcgtcg
      361 cgcgtggaaa aagaatacgg tcgccacggg gttgggcgac cgtattggga ccgagtatct
      421 ctcgatactt cgcccataat aacgtcacga gccgccgtag tccataacca attttagcta
      481 attattcgta acgcccgcca taaccgcgag tgctaccttt cgccgcggtc gaagtacgta
      541 cggctatgat cgttgttaca accgaaaacg tcgccggtta tcgcgtcgct caaacccttg
      601 gccaagtctt cggcgtggtc gtccgaagcc gcggtctcgg gggtaacatc gtggccggtc
      661 ttcgatcgat cgtcggcggt gaaattaccg agtataccga gatgctcgaa gaggcacgcc
      721 ggcacgccgt cgatcggatg gtccagaatg cgaccgccat gggcgccgac gccatcatca
      781 tgatgcgctt cgattcgtcc gagctcggcc aaacgatgag cgagatcgtc gcgtacggta
      841 cggccgttac gctgacggcg gcttcctgat gctgcgcgtc atcgtcatct tcttcggcgc
      901 cgctttgctg gccgtgtccg cagccatcgc gatcgcggcg cccggcgcgt ggcccgcggc
      961 cgtcgattgt gccgttctcg gcatcctgat tctcgtcgga acgctgctcg aaaaacgcta
     1021 ccgctcggcg cggcccgcga ccggtgccga ttggcaaacc accggcgagc gcttcatgga
     1081 cccgagcacc ggacagttca cgcaagtgcg ctacaaccgg caaaccggtg agaggtcgta
     1141 cgaaccctcc gacgcggaga gctttccaaa gatctgaccg cgggttccct ttatcgaggg
     1201 ggatcaggcc acgcgccggc cgcgaccgcc cgcctcagat cggcgtgcca agggatcgcg
     1261 aggtagcgct cgcggtcctc gggttcgagt gcgtcggcac tcgcctgcat cgcgcgctcc
     1321 gcgcgcgcga gcgtgcgtgc cgcctcggca gaacgtccgt cgcggcgctg gacttgcgcc
     1381 gccgcccagt agcagtacga cggccagtcg gttccgcgct ggatcgcatc gtcgtttgcg
     1441 agcagccgct gcacggcgtc gcgcgcggcc ggaagattcc cgagcgcggc ctgccagatg
     1501 gcgacgtcgg ccagcgtctt gctcgaccaa acctgcgact ctgagcgcga acgaacttcg
     1561 aacgaagcgt cggcgagtt
//
LOCUS       DASUCJ010000180         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1147958, whole genome shotgun sequence.
ACCESSION   DASUCJ010000180 DASUCJ010000000
VERSION     DASUCJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147958"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VFU90_02290"
     CDS             complement(<1..1381)
                     /locus_tag="VFU90_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5478627.1"
                     /translation="MSMWEPFTERARRSIVLAQEEAQRLGNNYIGTEHILLGIISEGE
                     SLAAKVLETLGVNLAKVRQEVEAIVGRGGQTVQQEMVFTPRAKRVIELAFEEARQLNH
                     NYIGTEHLLLGLIREGEGVAARVLTNLGVDPAKVRVQTTSLLGAEGQPPAPKGKSKTP
                     TLDAYGRDLTQLARENKLDPVIGRNNEIERVIQILSRRTKNNPALIGEPGVGKTAIAE
                     GLAQRVVKGDIPEPLRDRRVITLDLAGLVAGTKYRGEFEERMKRVMDEIRAATGEIIL
                     FIDELHTLVGAGAAEGAIDASNIIKPALARGELQCIGATTLNEFRKHIEKDSALERRF
                     QPVMVGEPSVEETVEILKGLRDRYEAHHKVQITDEALAAAAKLSDRYITDRFLPDKAV
                     DLVDEASSRVRLQATLPPPEVREIDAQLRAIVTEKETVVKNQEFDKAAAIRDREEKLR
                     LERARLEQEW"
     gene            1537..1848
                     /locus_tag="VFU90_02295"
     CDS             1537..1848
                     /locus_tag="VFU90_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEU5478628.1"
                     /translation="MPTVEQVREALVEVKDPELNLGVLELGLIYDVSVEGESGEHVNV
                     MMTLTSPMCPVGPMFQKSVEDHVLGVEGVKTAKVEITFTPPWDPRTMASDDVKVLLGI
                     W"
     gene            complement(1856..>2715)
                     /locus_tag="VFU90_02300"
     CDS             complement(1856..>2715)
                     /locus_tag="VFU90_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5478629.1"
                     /translation="SPISSAAYTSSEVEARSADGTMVPLSLVYRKGIKRDGSHPVDLE
                     GYGAYGITEDPYFSTTRVAWMERGGIYAVCHSRGGGWYGDAWHTAGMIATKNHTWEDF
                     IACGQWLVDHKYTSKAHLGGEGTSAGGISIGRAITTRPDLFAAALDVVGASNALRSEF
                     TPNGPPNVPEFGSVKTEAGFRALYAMDAYQHVVDGTAYPGVMLVTGYNDPRVSSWELA
                     KFAARLQQASSSGRPILLRVDYDAGHGFLAASRHQTEELLTDEYAFLLWQLGDPAFAG
                     IPKDIVPKK"
BASE COUNT          411 a          884 c          863 g          557 t
ORIGIN      
        1 gccactcttg ttcgagacgg gcgcgttcca gccgcagctt ctcttcacgg tcgcgaatcg
       61 cggcggcttt gtcgaactct tgattcttga cgaccgtttc tttctcggtc acgattgccc
      121 gcagctgtgc gtcgatttcg cgcacttccg gcggcggcag cgtggcctgc aggcgcaccc
      181 gcgagctcgc ttcgtccacc aagtcgacgg ctttgtccgg cagaaaacga tcggtgatgt
      241 aacggtcgct caacttggcg gcggccgcca gcgcctcgtc ggtgatttgc accttgtggt
      301 gcgcttcgta ccggtcgcgc agccccttga gaatttcgac cgtttcctct accgacggtt
      361 cgccgaccat caccggctgg aagcggcgtt cgagcgccga gtctttttcg atgtgtttgc
      421 ggaactcgtt gagggtcgtc gcgccgatgc attgcagctc gccgcgcgcc agcgccggct
      481 tgatgatgtt gctcgcgtcg atcgcgccct cggcggcgcc cgcgccgacc agcgtgtgca
      541 gctcgtcgat gaacaggatg atctcgccgg tcgcggcgcg gatctcgtcc atcacgcgct
      601 tcatgcgctc ttcgaattcg ccgcggtact tcgtgccggc caccaaaccc gccaggtcca
      661 gcgtgatgac gcgccggtcg cgcagcggct ccggaatatc gcccttgacg acgcgctggg
      721 ccaagccctc ggcgatcgcc gttttgccga cgcccggttc gccgatcagc gccggattgt
      781 tcttcgtgcg ccgcgacaaa atttgaatga cgcgttcgat ttcgttgttg cggccgatga
      841 ccggatcgag cttgttctcg cgggccagct gcgtgaggtc gcggccgtac gcgtcgaggg
      901 tcggcgtctt gctcttgccc ttgggggcgg gcggctgccc ttccgcaccc agcagcgagg
      961 tggtttgcac ccgcaccttc gcggggtcga cgcctaaatt ggtcagcacg cgagcggcga
     1021 cgccctcgcc ttcgcggatc agcccgagga gcagatgctc ggtcccgatg tagttgtggt
     1081 tgagctgacg cgcttcttcg aaagccagct cgatgacgcg tttggcgcgc ggcgtgaaga
     1141 ccatctcctg ctgcaccgtc tgcccacccc ggccgacgat cgcttcgact tcctggcgca
     1201 ctttggccag gttgacgccc aaggtttcga gcactttggc ggcaaggctt tcgccctcgg
     1261 aaatgatgcc gagcagaatg tgctcggttc cgatgtagtt gtttccgagg cgttgggctt
     1321 cttcttgagc gagtacgata ctgcgccgcg ctcgctcggt gaagggctcc cacattgaca
     1381 tcgctgggct gtttcctttc cgcgcgttac ctctttaact ctactcccgg cccgctagtt
     1441 tcgtgacggg cccgcctggt cggttgttct aacctacctt gtatctatcc ccgcattcca
     1501 gggttttacc ctttggagga cggaatcgag acacccatgc cgacggtcga gcaagtgcgc
     1561 gaggcgctgg tcgaagtaaa ggaccccgag ctgaatctcg gggtgctcga gctcggcttg
     1621 atctacgacg tctcggtcga gggcgagagc ggcgagcacg tcaacgtcat gatgacgctg
     1681 acgtcgccga tgtgcccggt cggcccgatg tttcagaagt cggtcgaaga ccacgtgctg
     1741 ggcgtcgagg gtgtcaagac ggccaaggtc gagatcacct tcacgccgcc gtgggaccct
     1801 cgcacgatgg cgtccgacga cgtgaaggtg ctgctcggaa tctggtagcc ggcggctact
     1861 tcttcggcac gatatccttc gggatgccgg cgaacgccgg atcgcccagc tgccacagca
     1921 agaacgcata ctcgtcggta agtagctctt cggtctgatg gcgcgacgcc gccaggaagc
     1981 cgtgtcccgc gtcgtagtcg acccgcagca gaatcgggcg cccgctcgac gacgcttgct
     2041 gcagccgcgc cgcgaacttc gccaattccc acgatgagac gcgcggatcg ttgtagccgg
     2101 taaccagcat cacgcccggg taagccgtgc cgtcgacgac gtgctggtag gcgtccatcg
     2161 catagagcgc gcggaatccc gcctcggtct ttacgctgcc gaactccgga acgttgggcg
     2221 gaccgttggg ggtgaactcg gagcgcagcg cgttgcttgc gccgacgacg tcgagcgccg
     2281 ccgcgaatag atccgggcgc gtcgtgatgg cgcggccgat cgagatgccg cccgcgctcg
     2341 tcccttcgcc gcccaagtgc gccttcgagg tgtatttgtg atccacgagc cactgtccgc
     2401 aggcgatgaa gtcttcccag gtgtggttct tggtcgcgat cataccggcg gtgtgccacg
     2461 cgtcgccgta ccagccgccg ccgcgcgaat ggcacactgc gtagatgccg ccgcgctcca
     2521 tccacgcgac ccgcgtcgtg ctgaagtagg gatcctcggt aatgccgtag gctccgtaac
     2581 cttcgagatc gaccggatgc gagccgtcgc gcttgatacc tttacggtac accagcgaca
     2641 gcggcaccat cgtgccgtcg gcgctgcgcg cctcgacttc cgacgacgtg tacgccgccg
     2701 agctgatggg cgaga
//
LOCUS       DASUCJ010000181         1216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_307686, whole genome shotgun sequence.
ACCESSION   DASUCJ010000181 DASUCJ010000000
VERSION     DASUCJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307686"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="VFU90_02305"
     CDS             <1..>1216
                     /locus_tag="VFU90_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478630.1"
                     /translation="TMPDGQGQTGSFSLLSAKSIEVLRGPFSALYGNASGGVISVFTE
                     DPSHEPVATVTGGGGSYGQWTFGTKLSATHDRVGYVVAGSEFQTSGYRDHSRARRDLT
                     NAKLVLSFDASTRITVIGNTQYQPETEDPLGLTRAEWSENPRGVDHAAILFDTRKTIN
                     QAQGGVALDHALSDALELHVDGYGGRRLVRQYLAFTGSGDTSSGGITDLDGNFGGVGA
                     RLVWRAVALGRPLTLTVGGDWNRQDQLRRGFVNDFGAMGDLRRDETDDVTGNDAYAQA
                     EWEFARRWSATAGVRTSSVRYTSVDHYVTGANGNDSGNRDFSNTSPVAGVVFHATDNV
                     NLYTSYGEGFEAPTFTEIAYRDNGTGLNFALQPATSRAFEVGAKFLWPDGQRLNLAWF
                     DTDTHNEIVVDQA"
BASE COUNT          211 a          402 c          425 g          178 t
ORIGIN      
        1 acgatgccgg acggccaggg gcagaccggc agcttcagcc tgctgtcggc gaagagcatc
       61 gaagtcctgc gcggcccgtt ctcggcgctc tatggcaacg cgtcgggcgg cgtgatctcg
      121 gtgttcaccg aggatccgtc ccacgagccc gttgcgacgg tgaccggcgg cggcgggagt
      181 tacggccagt ggacgttcgg cacgaagctc tcggcgacgc acgaccgcgt cggctacgtc
      241 gttgccggca gcgaattcca gacgagcggg tatcgcgacc actcgcgtgc gcggcgcgat
      301 ctcacgaatg cgaagctcgt gctgagcttc gatgcgtcga ctcgcatcac ggtgatcggc
      361 aacacgcagt accagcccga gaccgaggat ccgctgggcc tgacgcgagc cgagtggtcg
      421 gagaatccgc gcggcgtcga ccacgccgcg atcctgttcg acacccgcaa gacgatcaac
      481 caggcgcagg gcggcgtcgc gctcgatcac gcgctctcgg acgccctcga gctccacgtc
      541 gatggctacg gcggccggcg cctcgtgcgc cagtacctcg cgttcacggg ctcgggggac
      601 acgtcttcgg gcggcatcac cgatctcgac ggcaacttcg gcggtgtcgg cgcgcggctc
      661 gtctggcgtg cggtggcgct ggggcggccg ctgacgctca ccgtcggcgg cgactggaac
      721 cggcaggatc agttgcgccg gggcttcgtc aacgacttcg gtgcgatggg agacctgcgg
      781 cgcgacgaga ccgacgacgt gacgggcaac gatgcctacg cgcaggccga atgggaattc
      841 gcacggcgct ggtcggcgac cgcgggagtg cgcacgagca gcgtgcgcta cacgtcggtc
      901 gatcactacg tgacgggcgc gaatggcaac gacagcggca accgcgactt cagcaacacg
      961 agcccggtcg ccggggtcgt cttccacgca accgacaacg tcaatctcta cacgagctac
     1021 ggcgaaggat tcgaggcgcc gacgttcacc gaaatcgcgt atcgcgacaa cggcaccggg
     1081 ctcaacttcg cgctgcagcc ggcaacgagc cgcgcgttcg aggtgggcgc gaagttcctg
     1141 tggcccgatg ggcagcggct gaacctcgca tggttcgata ccgacacgca caacgagatc
     1201 gtcgtcgatc aggcga
//
LOCUS       DASUCJ010000182         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_139635, whole genome shotgun sequence.
ACCESSION   DASUCJ010000182 DASUCJ010000000
VERSION     DASUCJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139635"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /gene="topA"
                     /locus_tag="VFU90_02310"
     CDS             complement(<1..1351)
                     /gene="topA"
                     /locus_tag="VFU90_02310"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5478631.1"
                     /translation="MKKPLIIVESPTKARTIKKFLPSRLTVRASVGHVRDLPKSTLGV
                     DVDHDFAPKYLTIKGKGDVIKELKSAVKTASDVYLATDPDREGEAIAWHLAELLKLPQ
                     PLRIELHEITKDAALAALREAHAIDMDRVNAQQARRILDRLVGYKISPLLWAKVRGGL
                     SAGRVQSVAVKLIVDREREISAFVPREYWTLTALLAAADAPAMPFPAELVAHRGEKLE
                     LTGKAQADAALAGVEGAAFRVASIKKREVRRNAPAPFTTSTLQQEASRKLKIRVRRAM
                     QIAQALYEGVDLGGSEGTVGLITYMRTDSTRISDQARDAAREYIVSAYGEAFHGGRRH
                     KIREGAQDAHEAIRPTSVHLTPQKVAGALKRDELRLYTMIWERFVASQMAAAVLDQTT
                     VDIDANEYTFRATGTVTRFPGFTRVYEEGKDEDHGGAGEASQNGRTGAKGKPRVALPD
                     "
     gene            1569..1931
                     /locus_tag="VFU90_02315"
     CDS             1569..1931
                     /locus_tag="VFU90_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478632.1"
                     /translation="MMNGGTMAEEGRVRRKVRKARRKVRKAVRKAVVRKAVRKAVRKV
                     RRKKAARKVVRKVRRAVRKVRRKKAVRKAVRKVRRKKAARKVTRKVRRKVRKGARKVR
                     KAVRKGARKVRKAARKKK"
     gene            complement(2002..>2766)
                     /locus_tag="VFU90_02320"
     CDS             complement(2002..>2766)
                     /locus_tag="VFU90_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5478633.1"
                     /translation="RRLRRAVQIIFQDPFASLNPRMTVGDAIAEPLRIHGIVKGKAVE
                     ERVMDLLRRVGLRTYHANRYPHEFSGGQRQRVGIARALAVDPKFIVCDEPVSALDVSI
                     QAQVINLLEDLQQQFGLTYLFIAHDLSVVRHISTRVAVMYVGKLVETADRDALYLNPL
                     HPYTQALLSAIPIPDPALEKRRKRIVLVGDIPSPVNPPSGCRFHTRCPIAFEPCATLE
                     PPFKEYAPGHFAACHWVEQHGGQAPNLAGGPVVLAS"
BASE COUNT          492 a          868 c          937 g          469 t
ORIGIN      
        1 ggtcgggcag cgcgacgcgc ggcttgcctt tcgcgccggt ccggccgttt tggctcgcct
       61 cgccggcgcc gccgtgatcc tcgtctttgc cctcttcgta gacgcgggtg aatccgggga
      121 agcgcgtgac ggtaccggta gcgcgaaacg tgtactcgtt ggcgtcgatg tcgacggtgg
      181 tctgatccag caccgcagcg gccatttgcg acgccacgaa acgctcccaa atcatcgtgt
      241 aaaggcgcag ctcgtcgcgt ttgagcgctc cggctacctt ttgaggcgtg aggtggacgg
      301 acgtcgggcg aatcgcttcg tgagcgtcct gcgctccctc gcgtatcttg tggcggcgcc
      361 cgccgtggaa cgcttcgccg taagcggaaa cgatgtactc ccgggccgcg tcgcgcgcct
      421 gatcgctgat ccgggtcgaa tcggttcgca tgtaggtgat caggccgacg gtgccctcgc
      481 tcccgccgag atcgacgcct tcgtacagcg cctgcgcgat ctgcatggcg cggcgcaccc
      541 gaatcttgag ctttcgcgac gcttcctgtt ggagcgtcga ggtggtgaaa ggcgccggcg
      601 cgttgcgccg cacctcgcgc tttttgatcg acgcgacgcg aaacgcggcg ccttcgacgc
      661 cggccagcgc ggcgtcggcc tgggccttgc cggtgagttc gagcttctca ccgcgatgcg
      721 ccaccagctc ggccgggaac ggcatcgccg gcgcatcggc ggcggcgagc agcgcggtca
      781 gggtccagta ttcgcgcgga acgaaagccg atatctcgcg ctcccggtcg acgatcagct
      841 ttaccgcaac cgactgaacg cggccggccg acagaccgcc gcgcaccttc gcccatagca
      901 gcggggagat tttgtagccg accaggcggt cgagaatgcg tcgcgcctgc tgcgcgttaa
      961 cgcgatccat gtcgatcgcg tgcgcctcgc ggagcgccgc cagggcggcg tctttggtga
     1021 tttcgtgaag ctcgattcgc agcggctgcg gcagcttcag cagctccgcc agatgccagg
     1081 cgatcgcttc gccctcgcga tcggggtcgg tcgcgagata gacgtcggag gcggtcttta
     1141 cggcgctttt aagctctttg atgacgtcgc ccttaccttt aatcgtaagg tacttgggag
     1201 cgaagtcgtg gtcgacgtcg acgcccagcg tgctcttggg caggtcgcgc acgtgaccca
     1261 ccgatgcccg caccgtcaac cgcgacggca gaaacttctt aatcgtccgc gccttggtgg
     1321 gcgactccac gatgatcagg ggctttttca cggggcctag tatactccgc gggtcaagaa
     1381 gagggcaacg ccgagccgcc cgcacgcgcg cgtttttcgc cgatacgcgc gcgctcgcgc
     1441 gcgtacgcga atttgcatca tttggtgaaa tcctctatga ttcgcctcag agcgccgctg
     1501 tgagcgctgt ccaatacgat gcttgtcgtc ccccggggga cgaggaggaa tgaatggact
     1561 ccgataccat gatgaacggc ggaacgatgg ccgaggaagg gcgcgtgcgc cgcaaggtcc
     1621 gcaaagcccg acgcaaggtc cgtaaagccg tgcgtaaggc cgtcgttaga aaggccgtcc
     1681 gtaaggccgt gcgcaaagtg cgccgcaaaa aggcggcacg gaaggtggtc cgcaaggtcc
     1741 gcagagccgt tcgcaaagta cggcgtaaaa aggcggtccg caaggccgta cgcaaggtcc
     1801 gccggaaaaa ggcggcacgc aaagtcacgc gcaaggttcg cagaaaagtc cgcaagggcg
     1861 cgcgcaaagt ccgcaaggcc gtccgcaagg gcgcgcgtaa ggtccgcaaa gcggcgcgta
     1921 agaaaaaata ggctctaggc aacgcctaac gaaaagcggt cgcgaattcg cgaccgcttt
     1981 ttcatttgcg cgcgggccgg actacgaggc gagcacgacc gggccgccgg ccaaattggg
     2041 cgcctggccg ccgtgctgct ccacccaatg gcacgcggca aaatgacccg gcgcgtactc
     2101 cttgaacggc ggctcgagcg tggcgcacgg ctcgaacgcg atcgggcagc gcgtgtggaa
     2161 gcggcagccc gacggcggat tgacggggga cggaatgtcg cccaccagca cgatccgctt
     2221 gcggcgtttc tcgagcgccg gatcgggaat cggaatggcc gacagcaggg cctgcgtata
     2281 ggggtgcagc gggttgagat agagggcgtc gcggtcggcg gtttccacca gcttgccgac
     2341 gtacatgacg gccacccggg tggaaatgtg ccgcacgacc gagagatcgt gggcgatgaa
     2401 caggtacgtc agcccgaact gctgctggag gtcttcgagc aggttgatga cctgcgcctg
     2461 aatcgagacg tcgagcgccg agactggttc gtcgcagacg atgaacttcg gatcgaccgc
     2521 cagcgcccgc gcgatgccga cgcgctgccg ctgcccgccc gagaactcgt gcggatagcg
     2581 attggcatga taggtgcgca aaccgacccg ccggagcaga tccatcacgc gctcctcgac
     2641 ggccttgcct ttgacgattc cgtgaatccg cagcggttcg gcgatggcgt cgccgacggt
     2701 catcctcgga ttgagcgacg cgaacggatc ctgaaagatg atctgcaccg cgcggcggag
     2761 ccgccg
//
LOCUS       DASUCJ010000183         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_842547, whole genome shotgun sequence.
ACCESSION   DASUCJ010000183 DASUCJ010000000
VERSION     DASUCJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842547"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFU90_02325"
     CDS             complement(<1..1391)
                     /locus_tag="VFU90_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5478634.1"
                     /translation="MQTIHAFCTQLLHLFPFEAAVPARFEVLDEATETQMLGRLVLDV
                     LLEAAEAPDSALGRALQHAMLAAADQTFQDMLREVIRLRDPLMGWVAEAGGVTEAMAQ
                     LARALGVRPDETVVEVQAAMFDDSVLAASEWAAIAAALKEGSKTDKEQGFRFHALASL
                     AAPDRIEAYIDIFCTKKRDKTKERIATSAVEKSDPDLCRRLNDERERVWRLVQRQRAV
                     ETRDRSSALFTIAYAVIARFRAEKNRRGLLDYDDLIDKTLDLLRDQRAAWVHYKLDRG
                     IHHVLIDEAQDTSPKQWDIVKALVSEFFAGIGAHERERTIFAVGDEKQSIFSFQGAQP
                     REFAATRKHFEREHARAGLEFVATEFKHSFRSGPVVLGAVDTVFARPEAHNGLSADAV
                     APVHEALPDSAPGLVEIWDLETSDSFEKKEGWSAPFDVQSVASATAKLARRIARTVAI
                     WQQQGRRPKDVLVL"
BASE COUNT          220 a          466 c          453 g          256 t
ORIGIN      
        1 aggaccagca cgtccttcgg ccgccggccc tgctgctgcc agatcgcgac ggtgcgcgcg
       61 atccggcggg caagcttcgc ggtcgcgctg gcgacgctct gcacgtcgaa cggggccgac
      121 catccctcct tcttctcgaa ggagtccgat gtttccaggt cccatatttc caccagtccc
      181 ggcgcgctgt ccggcaaagc ctcgtgaacc ggcgcgaccg catcggccga aagaccgtta
      241 tgggcttcgg gacgcgcgaa caccgtatcg acggcgccga gcacgaccgg tccggagcgg
      301 aacgagtgct tgaactcggt agcgacgaat tcgaggccgg cgcgcgcgtg ctcgcgttcg
      361 aaatgcttcc gcgtcgcggc gaattcgcgc ggctgcgcgc cttggaacga aaagatcgac
      421 tgcttctcgt cgccgacggc gaagatcgtt cgttcgcgtt cgtgcgcgcc gatgccggcg
      481 aagaactcgc tgaccagcgc cttgacgatg tcccactgct ttgggctggt gtcctgcgcc
      541 tcgtcgatga gcacatggtg gatgccgcgg tcgagcttgt agtgcaccca cgccgcccgc
      601 tggtccctga gcaggtcgag agtcttgtcg atcaggtcgt cgtagtcgag cagaccgcgt
      661 cgattttttt cggcacggaa gcgcgcgatc accgcgtatg cgatcgtgaa cagcgcgctg
      721 ctgcgatcgc gcgtttcgac ggcgcgctgg cgctgcacca gccgccacac gcgttcgcgc
      781 tcgtcgttca gccgccggca gaggtccggg tcggatttct cgacggcgct cgtagcgatg
      841 cgctccttgg tcttgtcgcg tttcttggtg cagaagatgt cgatataggc ctcgatccgg
      901 tccggcgccg cgaggctcgc caacgcgtgg aagcgaaaac cctgctcctt gtcggtcttc
      961 gaaccttcct tcagtgcggc cgcgatcgcc gcccattccg atgccgcgag cacactgtcg
     1021 tcgaacatcg ccgcctgaac ttcgacaaca gtctcgtcgg gacggacgcc gagcgcccgc
     1081 gcaagctgcg ccattgcttc ggtgacgccg ccggcttcgg cgacccagcc catgagcggg
     1141 tcgcgcaagc ggatgacctc gcgaagcatg tcctgaaacg tctggtccgc ggcagcgagc
     1201 attgcgtgct gcaacgcccg gccaagggcg ctgtccggtg cttccgccgc ctcgagcagg
     1261 acgtcgagga ccagcctgcc gagcatctgc gtttccgtgg cctcgtcgag cacctcgaag
     1321 cgcgccggga cggccgcctc gaatggaaac agatgcagaa gttgcgtgca gaaagcgtgg
     1381 atggtctgca cctta
//
LOCUS       DASUCJ010000184         1757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_460489, whole genome shotgun sequence.
ACCESSION   DASUCJ010000184 DASUCJ010000000
VERSION     DASUCJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460489"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFU90_02330"
     CDS             complement(<1..1569)
                     /locus_tag="VFU90_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5478635.1"
                     /translation="MQLTRKSDPIRGVAPRIVRSGAHRIATMDRRSFLKRSGLAAGVG
                     AFASQLPYGTMEKARAADDAGAVKREVHRTVCTHCSVGCAIDAVVENGVWVRQEPVFD
                     SPLNLGAHCAKGASIREHGHGDHRLKTPMKLVDGKYQKISWEQAIKEVGDKLLAIRKE
                     AGPDAVFWVGSSKHSNEQSYLLRKFVSFFGTNNMDHQARICHSTTVSGVANTWGYGAM
                     TNSYNDMGNTKCAFYIGSNAAEAHPVSLLHMLHAKENGAKMIVADPRFTRTAAKADEY
                     VRFRSGTDVALLFGMLYHIFKNNWQDDKYIHDRVYGMDKVREDVLAKWTPDKVEEVTG
                     VPEAQVYKVAEMMAKNRPSTLVWCMGQTQHSIGNAMVRASCIVQLALGNIGVPGGGAN
                     IFRGHDNVQGATDIGPNPDSLPGYYGVAAGAWKHFAAVWKVDYEWMKKQFASEVMMEK
                     PGMTVSRWIDGVLEKNDLIDQDSNLRAVVYWGHAPNSQTRGKEMVEAMKKLDLIVVVD
                     PYPSATAAMSAMVRK"
BASE COUNT          266 a          605 c          549 g          337 t
ORIGIN      
        1 cttcctcacc atcgcgctca tcgccgcggt cgccgacggg tacggatcga cgacgacgat
       61 caagtcgagc ttcttcatcg cctcgaccat ctccttgccg cgcgtctggc tgttcggagc
      121 gtgcccccag tagacgaccg cgcgcaggtt gctgtcctgg tcgatcagat cgttcttctc
      181 gagaacgccg tcgatccagc gcgacaccgt catccccggc ttctccatca tcacttccga
      241 ggcgaactgc ttcttcatcc actcgtagtc gaccttccac actgccgcga agtgcttcca
      301 cgcgcccgca gcaacgccgt aataccccgg cagcgaatcg ggattggggc cgatgtcggt
      361 cgcgccctgg acgttgtcgt ggccacggaa gatgtttgcg ccgccccccg gtacgccgat
      421 gttgccgagc gccagctgga cgatgcacga agcgcgcacc atcgcgttgc cgatcgagtg
      481 ctgcgtctgg cccatgcacc agacgagcgt cgacggccgg ttcttcgcca tcatctcggc
      541 gaccttgtag acctgcgcct ccggaacgcc ggtcacttcc tcgaccttgt ccggagtcca
      601 cttcgcgagc acgtcctcgc gcaccttgtc catgccgtag acgcggtcgt ggatgtactt
      661 gtcgtcctgc cagttgttct tgaagatgtg atagagcatg ccgaacagca acgcgacgtc
      721 ggttcccgag cggaagcgga cgtactcgtc ggccttcgcc gccgtgcgcg tgaagcgcgg
      781 atcggcgacg atcatcttgg cgccgttctc cttcgcgtgc agcatgtgca gcagcgacac
      841 cgggtgggct tcggcggcgt tgctgccgat gtagaacgcg cacttcgtgt tgcccatgtc
      901 gttgtacgag ttggtcatcg cgccgtagcc ccaggtgttg gccacgcccg agaccgtcgt
      961 cgagtggcag atgcgcgcct ggtgatccat gttgttcgtc ccgaagaacg agacgaactt
     1021 gcgcagcagg tacgactgct cgttgctgtg cttcgagctg ccgacccaga acacggcgtc
     1081 ggggcccgcc tccttgcgaa tcgcgaggag cttgtccccg acctctttga tcgcctgctc
     1141 ccacgagatc ttctggtact tgccgtcgac gagcttcatc ggcgtcttca gccggtgatc
     1201 gccgtgcccg tgctcgcgga tcgacgcacc cttcgcgcag tgcgcgccga ggttgagcgg
     1261 cgaatcgaat accggctcct ggcgcaccca gacgccgttc tcgaccacag cgtcgatcgc
     1321 gcagccgacc gagcagtgcg tgcagaccgt gcgatggacc tcgcgcttga ccgcgcccgc
     1381 gtcgtcggcg gcgcgcgcct tttccatcgt cccgtacgga agctggctcg cgaacgcgcc
     1441 gacgcccgcg gcgagacccg agcgcttgag gaagctgcga cgatccatcg tcgcgatcct
     1501 gtgcgcgccg gaacggacga tgcgcggggc gaccccgcgg atgggatcgg acttgcgcgt
     1561 cagttgcatg gcctgtctcc tgctggatgc cgaactggag cgacgggtcc gcctcgcgga
     1621 cacccgtcgg tgatcgtcat gacgagcact gcctagcgac gcgcggacgc gtagtaggca
     1681 cgaatatggt cggtttcccg gtagccccga gacgccgctt cgggctcagc cggcgtcacg
     1741 gccgcggcag tcccggc
//
LOCUS       DASUCJ010000185         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_93699, whole genome shotgun sequence.
ACCESSION   DASUCJ010000185 DASUCJ010000000
VERSION     DASUCJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93699"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFU90_02335"
     CDS             complement(<1..675)
                     /locus_tag="VFU90_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEU5478636.1"
                     /translation="MRLTPLIALACGLTLLPASTPSTPPGGLTGFSAAGARAERDWET
                     KFKAIPSPDSERAYMRHLSARPHHVGSPYDSANAQWILAHFKSWGLDAHIERFEVLFP
                     TPKERLVELVGPTHFRASLLEPTVPGDPTSSQHAEQLPTYNAYSADGDVTAPLVYVNY
                     GLPADYDELERHGVSVKGAIVIARYGGAWRGIKPKVAAEHGAIGCIIYSDPKDDGYFE
                     GQYFPEG"
     gene            complement(689..1195)
                     /locus_tag="VFU90_02340"
     CDS             complement(689..1195)
                     /locus_tag="VFU90_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478637.1"
                     /translation="MPDLLIRIKRATDGSAALTCVRADGSVTWQRQVGPLGAFFPTHD
                     LTHYAVETTLGYRQGFYGLIADGWEIADFETAKGDIPREAREAELVVGVFEMELRMGR
                     DWSGSELREQGERYASSTRPERARMPLPPLTDDDIARVRALRAAVFAQWEAVGPGDTL
                     ELAFSRPT"
BASE COUNT          264 a          519 c          533 g          257 t
ORIGIN      
        1 gccttcgggg aagtattggc cttcgaagta gccgtcgtcc ttggggtcgg aatagatgat
       61 gcagccgatg gcgccgtgtt cggccgcgac tttcggcttg atgccgcgcc atgctccgcc
      121 gtagcgggcg atgacgattg cgcccttcac cgacacgccg tggcgctcga gctcgtcgta
      181 gtcggccggc agaccgtagt tcacgtacac gagcggcgcc gtgacgtcgc cgtccgcgga
      241 atacgcgttg tacgtgggca gctgctcggc gtgctgcgac gaggtcggat cgcccggcac
      301 cgtgggttcg agcagcgacg cacgaaaatg cgtcggccca accagctcta cgagacgctc
      361 cttcggcgtc gggaacaaca cctcgaaccg ttcgatgtgc gcgtcgagtc cccacgattt
      421 gaagtgggcc aggatccatt gggcgttcgc gctgtcgtac ggcgagccga cgtggtgcgg
      481 ccgcgccgag aggtggcgca tgtaggcgcg ctcgctgtcc ggcgacggaa tcgccttgaa
      541 cttggtttcc cagtcgcgtt cggcacgggc gccggcggcg gagaatcccg ttaggccgcc
      601 gggcggcgtg gaaggcgtgg aggccgggag cagcgtcaac ccgcaggcga gagcaataag
      661 aggtgtgagt cgcatgcgct ggattctgtc acgtaggtcg cgagaacgcc agttccagcg
      721 tgtcgccggg accgacggcc tcccactgcg cgaacaccgc cgccctgagc gcgcggacgc
      781 gcgcgatgtc gtcatcggtc aacggcggca gcggcatccg ggcgcgctcg ggccgtgtgc
      841 tcgacgcata gcgctcgccc tgctcacgca actcggaccc cgaccagtcg cggcccatcc
      901 gcagttccat ctcgaacacc ccgacgacga gctcggcttc acgcgcctcg cgcggaatgt
      961 cgcccttggc ggtttcgaaa tcggcgatct cccatccgtc ggcgatcagc ccgtaaaagc
     1021 cctggcggta gccgagcgtg gtctcgaccg catagtgcgt caggtcgtgg gtgggaaaga
     1081 acgcgcctaa cgggccgacc tggcgctgcc aggtgaccga gccgtcggcg cgaacgcacg
     1141 tcagcgcggc ggacccatcc gtcgcgcgct tgatgcggat gagcaagtcg ggcatggggc
     1201 gatcagaatg gtttgtcggg gagctcgaga tccgccaacc gaggcttctg ctcggccacg
     1261 gtgcagcggc cggctccaga caagctagtc ggcggcggct gagtttgtga ctgcggcaat
     1321 ggcgaactgt acaggacgac tctgcgccac cgctcgccat tgaacacgtg cgcaagtccc
     1381 ccgatgcgac agcgcgtccc gattcgcgac gccaccgacc gcgcgatgcc ttacgcaagc
     1441 cggactaacg tgccacggtc tggcgcgaac gtcagatact cctatcttcg aaaccggtgt
     1501 caccccgcac cgccacactc ggaatcgagg accgcgcaca gatgcagctg atcatcgccg
     1561 taccggcgta tcg
//
LOCUS       DASUCJ010000186         1927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_918938, whole genome shotgun sequence.
ACCESSION   DASUCJ010000186 DASUCJ010000000
VERSION     DASUCJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918938"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..692
                     /locus_tag="VFU90_02345"
     CDS             432..692
                     /locus_tag="VFU90_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU5478638.1"
                     /translation="MEAISYSRTRQHLAEVMDKVSDDRAPVMITRQRGRPVVMLSLEE
                     YNALEETSHLLRSPRNAKRLLESIEQLKRGGGKVRRLVRDAD"
     gene            682..936
                     /locus_tag="VFU90_02350"
     CDS             682..936
                     /locus_tag="VFU90_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU5478639.1"
                     /translation="MPIRFSDAAWEDYLYWQGHDRALLKRINALIRDVQRSPFEGIGK
                     PEPLKHSFAGFWSRRIDGEHRLVYAIEGDTVMIAQCRYHY"
     gene            complement(1000..>1927)
                     /locus_tag="VFU90_02355"
     CDS             complement(1000..>1927)
                     /locus_tag="VFU90_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU5478640.1"
                     /translation="SRTLLFDLHTGDWDDELLRLFGIPRSLLPAVVDSSGVCATTTLD
                     GASIPIAGIAGDQQAALFGQACVEPGLAKNTYGTGCFLLLNTGSVAVASANNLLTTVA
                     WRRAGALEYALEGSVFIGGAVVQWLRDGLQIIRSAADVEVLAASVADNGGVCFVPAFT
                     GLGAPHWDAYARGAMFGLTRGSTAGHIARAALESIAFQSADVLDAMQKDAGIALTELR
                     VDGGAAANDLLMQFQADILGVPVARPTVLETTALGAAYLAGLAVRYWPDTNAIAANRL
                     VDRRFEPKMPRDRVDALRGDWKKAVERAKGWA"
BASE COUNT          343 a          622 c          649 g          313 t
ORIGIN      
        1 gatgcgttcc acaccgcggg gcttcgcatc gccacgtgga cggtgaacga gcccgagcgc
       61 gcacggaagc ttctgcagtg gggcgtcgat acggtgatca ccgacgccgt cgacctcatt
      121 tcgccggggg cggttcgctg acgagcttcc ctgtcggtgc atcccggaac catgggaagc
      181 cggcaaacgg caggacgtgc tcgccatccg gcaacggcag ccagaacccg tgggtggaaa
      241 catgggtcac tctcgggagc cgaagcgacg gttccatgcg ctgcggatga catgccggaa
      301 gttctaccct tcgcgcgcag gttcgatcgc cgcggtgata tcgcgtcacg tggcgcgtga
      361 gttcgggtac taccgggttt gaccattggc tcacattgta ctataatgcg tacattgatt
      421 ggagtgaaac gatggaagcg atcagctaca gccggacccg gcagcacctg gccgaggtca
      481 tggacaaggt aagcgatgac cgagcgcccg tgatgatcac gcgccagcgg gggcgtcctg
      541 ttgtcatgct gtcgctcgaa gagtacaacg cgcttgaaga aacttcgcat ctcctccgct
      601 ctccgaggaa cgcgaagcgt ctgctggaat cgatcgaaca gctcaagcgc ggcggcggga
      661 aggtccggcg cctggtgcgc gatgccgatt agattctccg acgccgcctg ggaggactat
      721 ctatactggc agggacacga cagagcgctg ttgaagcgaa ttaacgcact tatccgcgac
      781 gttcagcggt ccccgttcga gggaatcggc aaacccgaac ccctgaaaca cagcttcgcg
      841 ggcttctggt cgcgccggat cgacggcgag caccggctgg tgtacgcgat cgaaggcgat
      901 acggtgatga tcgctcaatg tcggtatcac tactgatgcg atcgctgacg tgatcgccga
      961 tgtgatcata gatggcaccg ccgccgtgat caccgcacct cacgcccagc ccttcgcgcg
     1021 ctccaccgct ttcttccagt cgccgcgaag cgcgtcgacg cgatcgcgcg gcatcttcgg
     1081 ctcgaagcgc cgatcgacga gccggttcgc ggcgatcgcg ttcgtgtcgg gccagtagcg
     1141 gacggcaagc cccgcgaggt acgccgcgcc gagcgcggtc gtttcgagca ccgtcgggcg
     1201 cgcgacgggc acgccgagga tgtcggcctg gaactgcatc aggagatcgt tcgccgcagc
     1261 gccgccgtcg acgcgcagct cggtcagcgc gatgccggca tccttctgca tcgcatcgag
     1321 gacgtcggcg ctctggaatg caatcgattc gagtgccgcg cgcgcgatgt gccccgcggt
     1381 cgacccgcgc gtcagcccga acatcgcgcc gcgcgcgtac gcgtcccagt gcggcgcgcc
     1441 gaggccggtg aacgccggca cgaagcagac gccgccgttg tcggcgacac tcgcggcgag
     1501 cacttcgacg tcggccgcac tgcggatgat ctgcagcccg tcgcgcagcc attgtacgac
     1561 cgcaccgccg atgaacacgc tgccctcgag cgcgtattcg agcgcgcccg cacggcgcca
     1621 cgcgacggtc gtaagaagat tgtttgccga cgcgacggcg acactcccgg tattgaggag
     1681 caggaagcag ccggtgccgt aggtgttctt cgcgagcccc ggctcgacgc aggcctggcc
     1741 gaaaagtgcg gcctgctggt cgccggcgat gcccgcaatc gggatcgacg cgccgtcgag
     1801 cgtcgtcgtc gcgcacacac cggacgagtc gacgaccgcg ggtaggaggc tgcgcgggat
     1861 gccgaagagg cgcaggagct cgtcgtccca gtcgccggtg tgcaggtcga agagcagcgt
     1921 gcggctc
//
LOCUS       DASUCJ010000187         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_32450, whole genome shotgun sequence.
ACCESSION   DASUCJ010000187 DASUCJ010000000
VERSION     DASUCJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32450"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFU90_02360"
     CDS             complement(<1..440)
                     /locus_tag="VFU90_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478641.1"
                     /translation="MSQVHRWRAFAVLAVSFFMTIVDLTIVNVALPTIGAKLHFSESN
                     LQWVVTAYGVSFGGFLLLGGRAADLLGRRRVLMVGLSVFTAASLACGLATTDGFLIAM
                     RGIQGLGAAIVLPAALSIVMNMFDEGAERNKALGIWGGIGAAGAT"
     gene            553..1125
                     /locus_tag="VFU90_02365"
     CDS             553..1125
                     /locus_tag="VFU90_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5478642.1"
                     /translation="MGAMEESDARIAALAARLFYEHGITATGVDALSKAAGISKRTLY
                     ERFGSKDALIAAAFKSLDDPVFDRFIGAAMRSSNDPRRQLERIFTELEIAVGSDEFRG
                     CPFINGIAELTEPDHPAVEVVRKHKDRVRRWMRDRAKAAGATNPDLLSRQLMVVFEGG
                     QAQSLVEHSTKPARDARGIAKVLIDAAIQS"
BASE COUNT          235 a          414 c          391 g          191 t
ORIGIN      
        1 gtcgcgccag ccgcgccgat gccgccccag atgccgagcg ccttgttgcg ttctgcgccc
       61 tcatcgaaca tgttcatgac gatcgagagc gcggctggca acacgatcgc ggcgccaagg
      121 ccctggattc cgcgcatcgc gatgagaaag ccgtcggtcg tcgccaaccc gcaggcgagc
      181 gacgcggccg tgaagacgct taggccgacc atgagaactc ggcgacgtcc aagcaggtcg
      241 gcggctcgac cgccgagcag aaggaacccg ccgaaactca cgccgtacgc cgtgacaacc
      301 cactgcaggt tcgactcgga gaagtgcagc ttggcgccga tcgtcggcaa cgcgacgttg
      361 acgattgtca ggtcgacgat cgtcatgaag aacgagacgg ccagcaccgc gaacgcgcgc
      421 cagcgatgga cttgagacat acgattctcc tgaggtgccg tgtcggtgaa aaccgatcgg
      481 ttttctgccg gagccgccga ctatacagac gcgatcaccg tcgcgccaca tatttggaaa
      541 ccgatcggta ccatgggtgc catggaggag tccgacgctc ggatcgcggc gctcgcggcg
      601 cggctgttct atgagcacgg catcaccgcg accggagtcg acgcgctgag caaagccgct
      661 ggcatctcca agcgcacgct ctacgagcgg ttcggcagca aggacgcgct catcgcagcc
      721 gcgtttaagt cgctcgacga cccggtgttc gatcgtttca tcggcgccgc gatgcgttcg
      781 agcaacgatc cgcgtcgcca gcttgagcgc atcttcaccg agctcgagat cgcggtcggc
      841 tccgacgaat tccgcggctg ccccttcatc aacggcatcg cagagctcac cgagcctgat
      901 cacccggccg tcgaggtggt gcgcaagcac aaggatcgcg tacgtcggtg gatgcgcgac
      961 cgcgcgaaag cggccggggc caccaatccc gacctgctct cgcgacagct gatggtggtg
     1021 ttcgagggcg gccaagcgca atcactggtc gagcactcga ccaagccggc gcgcgacgcg
     1081 cgtggcatcg caaaggtgct gatcgacgcc gctatccaaa gctagccaac ggttccaacc
     1141 acgacggccg cgcctcgctc gtcgtccgtc tcgattcggg cgcgcagcgc agctgactca
     1201 aacgcctgga cggcggccgc ggcctgccgt t
//
LOCUS       DASUCJ010000188         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_63006, whole genome shotgun sequence.
ACCESSION   DASUCJ010000188 DASUCJ010000000
VERSION     DASUCJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63006"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFU90_02370"
     CDS             complement(<1..985)
                     /locus_tag="VFU90_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEU5478643.1"
                     /translation="MALTIDEAGMAKTRERKLEIARRIHDIAVHEFGLASEDLIFDDL
                     TFTLATGEAEWVDSAAETIEGIRLIKRELPGVLTSLGVSNVSFGLAPAARAVLNSVFL
                     HHCVQAGLDMAIVNPAHIRPYAELSLEERALADDLVFNRRPDALQRFIEFSASADPAS
                     PGTADASAESDLAALGPDGRIHWQILHRKKDGIEDALDAAGVREQPIRVLNEVLLPAM
                     KEVGDKFGAGELILPFVLQSAEVMKRAVRHLEQFLERAEGYTKGKVVLATVYGDVHDI
                     GKSLVNTILSNNGYTVFDLGKQVPVNTIIDKAVEVGADAIGLSALLVSTSKQ"
BASE COUNT          158 a          345 c          317 g          185 t
ORIGIN      
        1 tctgcttcga cgtcgacacg agcagcgccg acaatccgat ggcgtcggcg cccacctcga
       61 cggccttgtc gatgatcgtg ttcaccggca cctgcttgcc gaggtcgaac accgtgtagc
      121 cgttgttcga gagaatcgtg ttcacgagcg acttgccgat gtcgtgcacg tcaccataga
      181 ccgtggcgag caccaccttc cccttggtgt atccctcggc gcgctcgagg aactgctcca
      241 gatgcctaac ggcgcgtttc atcacctcgg cgctctgcag cacgaacggc agaatcagct
      301 cgcccgcacc gaacttgtcg ccgacctctt tcatcgccgg cagcaggacc tcgttgagga
      361 cgcggatcgg ttgctcccgc acgccggccg cgtcgagtgc gtcttcgatg ccgtcctttt
      421 tgcggtgcag aatctgccag tggatgcggc cgtcgggccc gagcgccgcc aggtcggatt
      481 cggcggacgc gtcggcagtc cccggcgatg ccggatcggc gctcgccgag aattcgatga
      541 accgctgcaa cgcgtcgggg cgccggttga agaccaggtc gtccgccagc gcccgctcct
      601 cgagtgagag ctcggcgtac ggccggatgt gcgccggatt gacgatcgcc atgtcgagtc
      661 cggcctgcac gcagtgatgc agaaagacgg aattgagcac ggcgcgcgcg gccggcgcga
      721 gtccgaagct gacgttgctc acgcctaacg acgtcagcac gccgggcagc tcgcgcttga
      781 tcagtcggat gccctcgatc gtctccgccg cgctgtccac ccattccgct tcgcccgtcg
      841 cgagggtgaa ggtgagatcg tcgaaaatca gatcctcgct ggccaggccg aactcgtgca
      901 ccgcgatgtc gtggatccgc cgcgcgatct cgagcttccg ttcgcgcgtc ttcgccatgc
      961 ctgcttcgtc gatcgtgagc gccacgacgg ccgcgccatg cagct
//
LOCUS       DASUCJ010000189         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1239934, whole genome shotgun sequence.
ACCESSION   DASUCJ010000189 DASUCJ010000000
VERSION     DASUCJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239934"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1367
                     /locus_tag="VFU90_02375"
     CDS             273..1367
                     /locus_tag="VFU90_02375"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEU5478644.1"
                     /translation="MTLAAAVGLGACKSDDAKNNPPPPPPPPTAAASGQGDVCAGGGG
                     QDTDAVSAPFVTRKLEAGGYCLDPQGQPKTYGDQGKFSMDEVCTTAFDGECEVYKRFG
                     LDRVVVVRYVDGSGAPNSVEVNLSRFKTPDGAYAMFTKRAVADGDPARATVKPLAAGA
                     AGATSSSNAYVWRGQYLAELTFVTEDTKMTPAAMERANDASTNAIAKDIGSKLPGSAD
                     LLPAAAALPTASRLPLGIAYYPKDALSLTGIGPLAVGYYKDGDKRWRDVVILRPDVEG
                     AKEAFRAFKLKPGSSPLKATGDEGVLVTLQEAEDRAKAEYLVARKGTRVVAVGDEELV
                     MEPGMSNDKLAPLKLTRDEKLQKLVALLGS"
BASE COUNT          258 a          511 c          541 g          226 t
ORIGIN      
        1 cgggtcgccg agcgcgggtc cggcgcggcc cgacgtagca accttttctt cgagtccgca
       61 aaaaggtgcg agttactccc gacgaaggcc gagaaggggc gcgaaccggc gcgagtgcgg
      121 taaaacgcgg tccccccatg cgcctgcatc cgccgaggat tccctccgtt ctgctctctc
      181 gttccaccgt gctccgcgtc gcggcgccgg ttcccgcttc gcccgcgccg cgtcgcgctt
      241 cggcctccgc ggcgagaggg acccttgcgg ccatgacgtt ggccgcggcg gtggggctcg
      301 gcgcctgcaa gagcgacgac gccaagaaca atccgcctcc gcctccgccg cctcccacgg
      361 cggccgcttc ggggcagggc gacgtttgcg cgggcggcgg cggccaggac acggacgccg
      421 tgagcgcgcc gttcgtgacg cgcaaactag aggccggtgg ctattgcctc gatccgcagg
      481 gccagcccaa gacgtacggc gatcagggca aattctcgat ggacgaagtc tgcacgacgg
      541 cgttcgacgg cgaatgcgag gtctacaagc gcttcgggct cgatcgcgtg gtcgtggttc
      601 ggtacgtcga cggcagcggg gcgccgaata gcgtcgaagt gaacctctcg cgattcaaga
      661 cccccgacgg cgcttacgcg atgttcacga aacgcgctgt cgccgatggg gacccggcac
      721 gcgccacggt aaaacccctt gccgcaggcg cggccggcgc cacgagcagc agcaacgcgt
      781 acgtctggcg ggggcaatac ctcgcggagc tcaccttcgt caccgaagac acgaagatga
      841 ctcccgcggc gatggagcgc gcgaacgacg cttcgaccaa cgcgatcgcc aaagacatcg
      901 ggagcaagct gccgggatca gcggacctgt tgcccgccgc cgccgcgctg ccgacggcgt
      961 cgcgcttgcc tctcggcatt gcctactacc cgaaggacgc gctgagcctc acgggcatcg
     1021 gcccgctggc cgtcggctat tacaaggacg gcgacaagcg ctggcgcgac gtcgtgattc
     1081 tccggcccga cgtcgaaggg gccaaagaag catttcgtgc gttcaagctg aagccgggct
     1141 cgtcgccgct caaagcgacg ggggacgaag gcgtgctcgt cacgttgcaa gaggccgaag
     1201 accgcgcgaa ggcggagtat ctcgttgcgc gcaaaggcac ccgcgtcgtt gccgtcggcg
     1261 acgaagagct ggtgatggag ccgggaatgt cgaacgacaa gctcgcgccg ctcaagctga
     1321 ctcgggacga gaagctgcaa aagctcgtgg ctttgctcgg atcgtgagcg ggttctggct
     1381 cgacgcgcgc ctcgtccgtc tttcgcgcgc tgccgtcgga ggggctcatc gtcgcgtgtc
     1441 tcggtcgcga gcgccggcga gggggatggc gccgcggcgc cgaagcgaag tggacttcgc
     1501 tacgaaggcg ggagccgcgg tcgcgcggga atgagc
//
LOCUS       DASUCJ010000190         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_796734, whole genome shotgun sequence.
ACCESSION   DASUCJ010000190 DASUCJ010000000
VERSION     DASUCJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796734"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFU90_02380"
     CDS             complement(<1..1055)
                     /locus_tag="VFU90_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5478645.1"
                     /translation="MIEGDRVPADGVLLSQTNLSIDESLLTGESVPVRKSEAQPPPVA
                     MGNPGGNGLPFVFSGTMVVQGKGVGRVLETGRQTHIGRIGKALASIAPEAARVQAETA
                     RAVRRLAAVAAVLSVAVTLIFGFTRGTWLPAILVGITFAMAIMPEELPVVLSVFFGLG
                     AWRIAQKGVLTRYLPAIETLGSATVLCVDKTGTITQNRMTVAALASESGAIWQSGGDH
                     SLPDEFHEVLEFGVLASHRDPFDPTERAIAEALDEFLAGTEHVHRNWELAGEYPLSRD
                     MLATSRVWSASSNGRRVIAAKGAPEAIADLCHLPPDRVERLDRTVVGLASEGFRVLGV
                     ARAGFPLPELPPSQHDF"
BASE COUNT          182 a          367 c          346 g          166 t
ORIGIN      
        1 tcgaagtcgt gttgcgacgg cggcagttcg ggaagcggaa atccggcgcg ggcgacgccc
       61 agcacccgaa atccctctga cgccaaccct accaccgtgc gatcgagccg ttcgacgcga
      121 tccggcggga ggtggcagag atcggcgatc gcttcgggcg cgcctttggc cgcaatgacc
      181 cgccgcccgt tcgagctcgc cgaccagacg cgcgaggtcg cgagcatgtc gcgcgagagc
      241 ggatactcgc cggccagttc ccagttgcga tgcacgtgct ccgtgcccgc caggaactcg
      301 tcgagcgcct cggcgatggc gcgctcggtc gggtcgaagg ggtcgcgatg gctcgcgagc
      361 acgccgaact cgagcacttc gtggaattca tcgggtaagg agtggtcgcc accggattgc
      421 cagatcgcgc cgctttccga cgcgagcgcc gcaaccgtca tccggttttg ggtgatcgtt
      481 cccgtcttgt cgacgcacaa gacggtcgcc gaaccgagcg tttcgatggc gggcaagtaa
      541 cgcgtgagca cgcctttctg cgcgatcctc cacgcgccca gcccgaagaa caccgagagc
      601 acgaccggga gttcttcggg catgatcgcc atcgcgaacg tgatgcccac taaaatcgcc
      661 ggcagccacg taccgcgggt gaaaccaaag atcagcgtga cggcgacgct cagcaccgcg
      721 gcgacggcgg cgagccggcg taccgcacgg gccgtctcgg cttgaacccg cgccgcttcg
      781 ggcgcgatcg acgccagcgc cttcccgatc cggccgatgt gcgtctgccg gccggtttcg
      841 agcacccggc ccacgccttt cccctgcacg accatcgtgc cggaaaagac gaacggcaga
      901 ccgttccctc ccggatttcc catcgcgacc ggtggcggct gcgcctcgga cttgcgcacc
      961 ggcacggatt cgccggtcag cagcgattcg tcgatgctga gattggtttg cgagagcagc
     1021 acgccgtcgg ccggaacgcg atccccttcg atcaagacga t
//
LOCUS       DASUCJ010000191         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_674458, whole genome shotgun sequence.
ACCESSION   DASUCJ010000191 DASUCJ010000000
VERSION     DASUCJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674458"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFU90_02385"
     CDS             complement(<1..910)
                     /locus_tag="VFU90_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU5478646.1"
                     /translation="MNFKDYYAILGVPKNAAQKDVKSAYRKLARKWHPDANPQNAKEA
                     EEKFKEISEAYEVLGDAEKRKKYDLLGPDWQQAARQAEQQRRYRTGNEGVEFDFGDFA
                     GGHGAGGGPSGFSDFFDMFFSGVGRRQTTQSAGFARRGQDLETTIELGLRDVYDGGKK
                     AVSLQIEDVCPRCHGTGTESGRLCPQCHGTGHVLMVKKFEVSIPKGIGEGQRIRLAGQ
                     GGAGLNGGPNGDLHLIVRAIEDPTYKRKGDDLYVDLPVSIYDLLLGGEVKVPTLSGQV
                     AMTIPEGTQNNRLLRLSGKGIPHVKGG"
     gene            complement(907..1419)
                     /locus_tag="VFU90_02390"
     CDS             complement(907..1419)
                     /locus_tag="VFU90_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5478647.1"
                     /translation="MLRTEAGEAVLDESAAEEAAPLEERLAAAEARAEENYNKFLLAT
                     ADFENYKKRILRDRESIMAAYRRALLERFLPVFDNLERALKYEGGDGLRGGLEQTLKG
                     FEAVLASQGVKELDVKGKPFDPRLAEAIGTMRSDDAGDDTVVEVAEKGYSLGDELLRP
                     AKVIVAKSAE"
     gene            complement(1559..>2916)
                     /gene="dnaK"
                     /locus_tag="VFU90_02395"
     CDS             complement(1559..>2916)
                     /gene="dnaK"
                     /locus_tag="VFU90_02395"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5478648.1"
                     /translation="LVWDLGGGTYDVSILEVGDGVFEVKATNGDTRLGGDDYDHRVVE
                     WLVAEFRKEQGIDLSKDKQAMQRLTEAAEKAKIELSSVVQTNINLPFITADQEGPKHL
                     DYTLTRAKFEELTSDLTDRCVGPFKNAIADAKLDVSKIDEIIMVGGATRMPVIQELVR
                     KLTGKEPNLTVNPDEVVAVGAAIQAGVLAGEVRDVVLLDVTPLSLGLETLGGVMTKLI
                     DRNTTIPTKKSQVFTTAEDGQTSVDIHVLQGERPMAQDNKTLGRFRLEGIPAAPRGVP
                     QIEVTFNIDANGIVNVSAKDLGTGKEQAITISASTNLSKEEVDRMVKDAELQAATDKA
                     KIEEAEVRNSASSLIYSTEKSLKEVGEKLDASARGEVESALAELKRVSDNGTVAEIKP
                     AIETLQQASYKMAELLYKASTPEGEPAGAQADGAAGGNGASAGAAPSEDVIDAEFKES
                     K"
BASE COUNT          429 a          933 c          897 g          657 t
ORIGIN      
        1 cgccgccctt gacgtgcggg atgcctttgc cggaaagccg cagcagccgg ttattctgcg
       61 ttccttcggg gatcgtcatc gcaacctggc cgctgagcgt cggaaccttt acttcgccgc
      121 ccagcagcaa gtcgtaaatg ctcaccggaa ggtcgacgta gagatcgtca cccttgcgtt
      181 tgtaggtcgg atcttcgatc gcgcggacga tcaagtgtag atcgccgttc gggccgccgt
      241 tcaaaccggc gccgccttgc ccggccagcc ggatacgctg cccctcgccg attcctttgg
      301 ggatgctgac ttcgaatttc ttgaccatca gcacgtgtcc ggttccgtga cactgcggac
      361 agagccgtcc gctctcggtt ccggtgccgt gacagcgcgg acagacgtct tcgatttgca
      421 gcgaaaccgc tttcttgccg ccgtcgtaga cgtcgcgcag ccccagttcg atcgtcgtct
      481 ccaaatcttg tccgcggcgc gcgaacccgg cgctttgggt ggtttgacgc cggccgacgc
      541 ccgaaaagaa catgtcgaag aagtccgaga aaccgctcgg accgccgccc gctccgtgcc
      601 ctccggcgaa gtcgccgaaa tcgaactcga cgccttcgtt gccggtgcgg tagcgccgct
      661 gctgttcggc ttgccgcgcg gcctgctgcc aatccggccc gagcaggtcg tacttcttgc
      721 gcttctcggc gtcgccgagc acctcgtagg cctcggagat ctccttgaat ttttcctcgg
      781 cctctttggc gttctgcggg ttggcatcgg gatgccactt gcgcgcgagc ttacggtacg
      841 cagacttgac gtctttctgc gccgcgttct tgggaacgcc gagaatcgcg tagtagtctt
      901 tgaagttcat tcggcgcttt ttgcgacgat gaccttagcc ggccgcagca gctcgtctcc
      961 gaggctgtag cccttttcgg caacctcgac gaccgtatcg tcgccggcgt cgtcgcttcg
     1021 catcgtgccg atcgcctcgg ccaaccgggg atcgaacggc ttgcccttga cgtcgagttc
     1081 tttgacgcct tggctcgcca gcaccgcttc gaagcctttg agcgtctgtt cgagcccgcc
     1141 gcgcaagccg tcgccgcctt cgtatttcag cgcgcgttcg agattgtcga acacgggcag
     1201 gaaacgttcg agcagcgccc ggcggtacgc ggccatgatc gactcgcggt cgcgcagaat
     1261 tcgcttcttg tagttttcga aatccgccgt cgccagcaga aatttgttgt agttttcttc
     1321 ggcgcgagct tcggccgccg ccaagcgctc ttcgagcggc gccgcttctt cggcggcgct
     1381 ctcgtcgagg accgcttcgc cggcttcggt tctcaacacc gatggatcgt caaacattcg
     1441 tcacacacat tcgttaaggc aatcgtatgt cgcgggaacg gcgcgggccg caccccttcc
     1501 ggccgcgacg cgggtgcgcg cgaaccgaag gaagtgcgcg cccgcgccgc cgtaacgcct
     1561 acttcgactc tttgaactcg gcgtcgatga cgtcttcgga aggcgcggct ccggccgagg
     1621 cgccgtttcc gccggcggct ccgtcggctt gcgcgccggc gggttcgcct tcgggcgtcg
     1681 aggctttgta cagcagttcg gccatcttgt agctggcctg ctggagcgtc tcgatcgcgg
     1741 gcttgatctc ggcaaccgta ccgttgtcgc tgacgcgctt gagctcggcg agtgccgact
     1801 ccacctcgcc gcgggccgac gcatccagtt tttcgccgac ttccttgagc gatttttcgg
     1861 tggagtagat caggctcgac gcgctgttgc gcacttcggc ttcctcgatc ttcgccttgt
     1921 cggttgccgc ctgcagctcg gcgtccttga ccatccggtc gacttcctct ttggagaggt
     1981 tggtcgaagc gctgatcgtg atcgcctgtt cttttccggt gccgaggtct ttggcgctca
     2041 cgttgacgat gccgttggca tcgatgttga acgtgacctc gatctgcgga acgccgcgcg
     2101 gcgctgccgg gatgccttcc aggcggaagc gccccagcgt cttgttgtct tgcgccatcg
     2161 ggcgttcgcc ctgcagcacg tggatgtcga ccgacgtttg gccgtcttcg gccgtcgtga
     2221 agacttgcga cttcttggtg ggaatcgtgg tgttgcgatc gatcagcttg gtcatcacgc
     2281 cgccgagggt ttcgaggccc agcgagagcg gagtgacgtc gagcagaacg acgtcgcgca
     2341 cttcgccggc gagcacgccg gcttgaatgg cggcgcccac cgcgaccact tcgtcgggat
     2401 tgacggtgag gttcggctct ttaccggtga gcttgcgaac caattcctga atgaccggca
     2461 tgcgggtagc gccgccgacc atgatgatct cgtcgatctt ggagacgtcg agcttggcgt
     2521 cggcgatcgc gttcttgaac ggtcccacgc agcgatcggt cagatcgctg gtcagctctt
     2581 cgaatttcgc acgcgtcagc gtgtagtcga gatgcttggg gccttcctga tcggccgtga
     2641 tgaacggcag gttgatgttc gtctgcacga ccgacgagag ctcgatcttg gccttttcgg
     2701 cggcttcggt gagacgctgc atcgcctgct tgtctttgct gaggtcgatg ccttgctctt
     2761 tgcggaactc ggcaacgagc cattcgacga cgcgatgatc gtaatcgtcg ccgcccaaac
     2821 gcgtgtcgcc gttcgtcgct ttgacttcaa aaacgccgtc gccgacttcg agaatcgaga
     2881 cgtcgtacgt gccgccgccg agatcccaga ccaaaa
//
LOCUS       DASUCJ010000192         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_536878, whole genome shotgun sequence.
ACCESSION   DASUCJ010000192 DASUCJ010000000
VERSION     DASUCJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536878"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..458
                     /locus_tag="VFU90_02400"
     CDS             198..458
                     /locus_tag="VFU90_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478649.1"
                     /translation="MAKRSTVRRLADAFVALLTWVAAAAAVMWFYDWVSRWYVVVGVL
                     LVIVAGAIFGDRLKAARAASSEEVAELLPMQRESVEPVELAS"
     gene            complement(470..>1511)
                     /locus_tag="VFU90_02405"
     CDS             complement(470..>1511)
                     /locus_tag="VFU90_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5478650.1"
                     /translation="EHTTWGTITRVHPFPTGDKKNIPKRAVGFAGFCALTAAIAAAGR
                     RVDVTFAMTPPLPMAATGWLASLPRRAPLVLNVQDVFPDVAVELGVLRGQRVIAAASA
                     LERWSYARSAAVTVLSDDMRANLAAKMRHPTRLQVIPNFVDVESVRPLDKENSYRAEY
                     GLSGKTVVMYAGNVGMSQSLDLLVAAARETRDRDDVVYVVNGGGSTLADLQASAASLP
                     NMRFVPMQPYERLPEVLAAADVHVVPLKRELSRSSVPSKTYSILAAGRPVLASVDEGS
                     EVQRIVEAAGAGVAVPPEDERAFVTALRDLINDVERRTQMEKAARAYVENATSPRAVA
                     EAYESLFNSLRP"
BASE COUNT          241 a          520 c          485 g          265 t
ORIGIN      
        1 cgcccgccct gttcacgcgc gccctgttca atggtgtggg gcaacgcaga aggagcgacg
       61 aactagtcaa cctgggtggc agagcgggct gcgagtggtc cgttgtgggc aatctgccgt
      121 ttggctctgt ggtttgagcc acacgatccc gactcctcgt tatggtgcgg tcacccgcac
      181 gtggctcgag gagtcccatg gccaagcgct ccaccgtccg caggctcgct gacgcgttcg
      241 tcgcgctgct gacctgggtt gccgccgcgg ccgccgtcat gtggttctac gactgggtca
      301 gccgctggta cgtcgtcgtc ggcgtgttgc tggtgatcgt cgctggcgcg atctttggcg
      361 accggctgaa ggcggcacgc gccgcgtcgt ccgaagaagt tgccgaactg ctgccgatgc
      421 agcgcgaatc cgtggagccg gtcgaactcg cgtcttgatc ggttcgtgat cacggacgaa
      481 gcgagttgaa gagcgactcg taggcttcgg ccaccgcgcg cggcgacgtc gcgttttcta
      541 cgtaagcgcg ggcggccttc tccatctgag tgcgccgctc gacgtcgttg atcagatcgc
      601 gtagcgcggt gacgaacgcg cgctcgtcct ccggtggcac cgccactccc gcgccggccg
      661 cctcgacgat gcgttgcacc tcgctgccct cgtcgacgct ggccagcacc ggacgtccag
      721 ccgccagaat cgagtacgtc ttcgacggca cgctcgatcg cgacagctcc cgcttcagcg
      781 gcaccacatg gacgtcggct gccgccaaca cctcgggcaa tcgctcatac ggctgcatcg
      841 ggacgaaccg catgttgggc aggctcgccg ccgaagcctg tagatccgcc aacgtcgagc
      901 cgccgccgtt gacgacgtac acgacgtcgt cgcgatctcg ggtctcgcgc gccgcagcca
      961 ctagcagatc gagcgattgc gacatcccca cgttgcccgc gtacatcacc accgtcttgc
     1021 ccgacaaccc gtactcggcg cgataggaat tctctttatc gagcggccga acgctctcga
     1081 cgtcgacgaa gttcggaata acctgcaggc gcgtcggatg ccgcatcttc gccgcgagat
     1141 ttgcccgcat gtcgtccgac agcacggtaa cggcggcgct tcgcgcatat gaccaacgct
     1201 cgagcgcgga cgccgcggcg atcactcgct ggccgcgcag cacgcctaac tcgactgcca
     1261 cgtcaggaaa aacgtcctgc acattgagca cgagcggcgc gcgccggggc agggacgcca
     1321 accagcccgt cgcggccatc ggcaacggcg gggtcatcgc gaacgtgacg tcgacgcgtc
     1381 ggcccgccgc cgcgatcgcg gcagtcagcg cgcagaagcc ggcgaatccg accgcccgct
     1441 tgggaatgtt cttcttgtcg ccggtcggaa acggatgcac tcgcgtgatc gtgccccacg
     1501 tggtgtgctc t
//
LOCUS       DASUCJ010000193         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_873125, whole genome shotgun sequence.
ACCESSION   DASUCJ010000193 DASUCJ010000000
VERSION     DASUCJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873125"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFU90_02410"
     CDS             <1..717
                     /locus_tag="VFU90_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478651.1"
                     /translation="HDDAARIMEDIEARVQKETGQPLPPPQVLADEDESKKGSWAEIW
                     SPAFIGRTIVLIVFNLFQTVGYYGFASWVPTLLIAKGITVTKSLEYTFIIAIANPFGP
                     LIGRTFADRFERKWQVAWAAIAIAVLGLIFSQQTAGIWIIVVGILVTLANNWMSFAFH
                     AYQAELYPTRIRAQAVGFVYSWSRFSAIFSGFMIAFFLKQYGNTGVFSFIALCMVVVF
                     VVVGGFGPRTTRQRLEQIAR"
     gene            1050..>1811
                     /locus_tag="VFU90_02415"
     CDS             1050..>1811
                     /locus_tag="VFU90_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEU5478652.1"
                     /translation="MIIVMQPEAGEAEIAAVEAKIRSHGLEVHVSRGTERTLIGAIGD
                     ERRLDPEMFDPLPGVERSLHILKPYKLVAREWHKASSVVDVAGVPIGGSDVQVIAGPC
                     SVETVPQMEAAADGVAAAGARLMRGGAFKPRTSPYAFQGKGVEGLTMLKDAAQRRGLP
                     IVTELMDVRMLDTFLEHDVDVIQIGTRNMQNFDLLKEVGTVDVPVVLKRGMSATISEW
                     LMAAEYIAAGGNHRIILCERGIRTYESAYRNVLDVT"
BASE COUNT          308 a          571 c          631 g          301 t
ORIGIN      
        1 cacgacgacg cggcgcggat catggaggac atcgaagcgc gcgtgcagaa ggaaaccggc
       61 cagccgctgc cgccgccgca ggtactcgcg gacgaggacg agtcgaagaa gggctcgtgg
      121 gcggaaatct ggagccccgc gttcatcggc cggacgatcg tgctgatcgt gttcaacctg
      181 ttccagaccg tcggttacta cgggtttgcg agctgggtcc ccacactgct gatcgcgaag
      241 gggatcacgg tgacgaagtc gctcgaatac acgttcatca tcgcgatcgc caatccgttc
      301 gggccgctga tcggccgcac cttcgccgac cgcttcgagc gcaagtggca ggtcgcgtgg
      361 gcggcgatcg cgatcgccgt gctcggcctc atcttctcgc agcagactgc ggggatctgg
      421 atcatcgtcg tcggcatcct ggtgacgctc gccaacaact ggatgtcctt cgcgttccac
      481 gcctaccagg ccgagctcta tccgacgcgc atccgtgcgc aggcggtggg cttcgtctat
      541 tcatggagcc ggttctcggc gatcttcagc ggcttcatga tcgcgttctt cctgaagcag
      601 tacgggaata ccggcgtgtt cagcttcatc gcgctgtgca tggtcgtcgt gttcgtcgtc
      661 gtcgggggtt tcgggccgcg gacgacgcgc cagcggctgg agcagatcgc gcggtgagcg
      721 aggcttttgc gacggcgggt gcggcgcgca ttcgcgcgag gccgggagta gggcgtattc
      781 acgcggcgcc gggtgtggcg ccgatgcaac ccgccgcggc gcggcggccg gcgtccgccg
      841 ggcggatcaa cgcgccgggc gtaaatgctg ctacactgcc gggcatgacg cgagtgaccg
      901 ggttctgggg ctacttctac ttttcggatc acgccggggg ccgcgcgcac gcataagtca
      961 cacgaaatcg aacacagcgc atcgggcggc ccctcaaaag gccgcccgat gctttttgcg
     1021 ccatcgatct acccacgaag gagaaggaga tgatcatcgt catgcagccc gaggccgggg
     1081 aagccgaaat tgccgcggtc gaggcgaaga ttcgctcgca cggcctcgaa gtccatgtct
     1141 cgcgcggcac cgagcgcacg ctgatcggcg cgatcggcga cgagcgccgg ctcgatcccg
     1201 agatgttcga cccgcttccc ggcgtcgaac ggtcgctgca catcctgaag ccgtacaagc
     1261 tcgtcgcgcg cgagtggcac aaggcgagca gcgtcgtcga cgtcgccggt gtcccgatcg
     1321 gcgggagcga cgtgcaggtg atcgccgggc cgtgctcggt cgagacggtg ccgcagatgg
     1381 aagctgccgc cgatggtgtc gcggcagccg gtgcccgcct gatgcgcggc ggcgcgttca
     1441 agccgcgcac gagcccgtac gcgttccagg gcaagggcgt cgagggactc acgatgctga
     1501 aggacgcggc gcagcgccgg gggcttccga tcgtgaccga attgatggac gtgcggatgc
     1561 tcgacacgtt cctcgagcac gacgtcgacg tgatccagat cggcacgcgc aacatgcaga
     1621 acttcgacct cctgaaggag gtgggcacgg tcgacgtccc ggtggtcctg aagcgcggca
     1681 tgagcgcgac gatcagcgaa tggctgatgg ccgccgaata catcgcggcc ggaggcaatc
     1741 atcggatcat cctgtgcgag cgcgggatcc gcacctacga gagcgcgtac cgcaacgtgc
     1801 tcgacgtgac c
//
LOCUS       DASUCJ010000194         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1255271, whole genome shotgun sequence.
ACCESSION   DASUCJ010000194 DASUCJ010000000
VERSION     DASUCJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255271"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..>1341
                     /gene="nuoF"
                     /locus_tag="VFU90_02420"
     CDS             128..>1341
                     /gene="nuoF"
                     /locus_tag="VFU90_02420"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU5478653.1"
                     /translation="MPVTKVLTAGIGEADLRDIAVYRRRGGYQQWERAVRTLKPAEVL
                     DLAEKSGLRGRGGAGFPTGKKWSFLPADKNLRYLVCNCDEAQPGTFKDHMLLEEAPHL
                     VLEGMLLGSYGIACHHAFIYIRGEFKRGYEIFCAALEQARAAGYVGSNIFGSGFDLEV
                     TVQRGAGAYICGEETGLLNSLEGKRGEPRLKPPFPAIAGLYGMPTVVNNVETLAYLVP
                     ILEKGADWFAAVGTERSKGYKIVSVSGHVQRPGNYEVALGTPVREIIELAGGLRPGRS
                     LMAVQPGGGSSACIFEEHLDLPYDYESMTKAGSMLGSGAFVVFDDTTDFVKAAHNLVR
                     FFSHESCGQCTPCREGGSWLERVLERLVHHRGVAGDYEMLLRVAGTITGLNLCALGDS
                     IEPFLKSVVLRFP"
BASE COUNT          228 a          422 c          461 g          230 t
ORIGIN      
        1 gaccggagcg gagtaatcat gctcgacaac atcgtcgcgc gcgacgtcta ctttttcaaa
       61 cggacgcgcg agcgcgcggt cgcggacgcg cgcgccatcg tcgacgccga aacggaggcg
      121 cagcccagtg ccggtcacta aggttctcac cgccggcatc ggcgaggccg accttcgcga
      181 catcgcggtg tatcgccggc gcggcggcta ccagcaatgg gagcgtgcgg tgcgcacgct
      241 caaaccggcc gaggtgctcg atctcgccga gaagtcgggg ctgcgcggac gcggcggcgc
      301 cggattcccg accgggaaga agtggtcgtt cttgccggcc gataaaaact tgcgctatct
      361 cgtctgcaac tgcgacgaag cgcaaccggg cacgttcaag gaccacatgc tgctcgaaga
      421 ggcgccgcat ctggtgctgg aaggcatgct gctgggctcg tacgggatcg cgtgccacca
      481 cgcgttcata tacattcgcg gcgagttcaa acgcggctac gagatcttct gcgcggcgct
      541 cgaacaggcg cgcgcggccg gctacgtggg cagcaacatc ttcgggagcg gattcgactt
      601 ggaggtcacc gtgcagcgcg gcgcgggcgc ctatatttgc ggcgaagaga cggggctgct
      661 caactcgctc gaaggcaagc ggggcgagcc gcgcctgaag ccgccgtttc cggcgattgc
      721 cgggctctac gggatgccga cggtggtcaa taacgtcgag acgctcgcct atctcgtgcc
      781 gattctcgaa aaaggcgcgg actggttcgc ggcggtggga accgagcgaa gcaaaggcta
      841 caagatcgtt tcggtctcgg ggcacgtgca gcgccccggc aactacgagg tcgcgctcgg
      901 aacgccggtc cgcgaaatca tcgaactcgc cggcggcttg cgtccggggc gctcgctgat
      961 ggccgtgcag ccgggcggcg gttcgtcggc gtgcatcttc gaagagcatc tcgacttgcc
     1021 gtacgactac gagagcatga ccaaggcggg ctcgatgctc ggctccggtg cgttcgtggt
     1081 attcgacgac acgaccgact tcgtcaaggc ggcgcacaac ctggtccgct ttttctccca
     1141 cgaatcgtgc ggtcagtgca cgccgtgccg ggagggcgga agctggctcg agcgcgttct
     1201 cgagcggctc gtgcatcatc gcggcgtcgc cggcgactac gagatgctgc tgcgcgtcgc
     1261 cggcacgatt accggcttga acctgtgcgc gctcggcgac tcgatcgaac ccttcttgaa
     1321 gtcggtcgtg ctgcgctttc c
//
LOCUS       DASUCJ010000195         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1148081, whole genome shotgun sequence.
ACCESSION   DASUCJ010000195 DASUCJ010000000
VERSION     DASUCJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFU90_02425"
     CDS             complement(<1..251)
                     /locus_tag="VFU90_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478654.1"
                     /translation="MHTKTPVGEAWRCLRCGTFVLGEPKGSGPAEDAPIVLRGRALRD
                     AFVLRLLAAERLLRGVAMGLGSWAVLRYAHSENALRTLF"
     gene            246..869
                     /locus_tag="VFU90_02430"
     CDS             246..869
                     /locus_tag="VFU90_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5478655.1"
                     /translation="MQAVAQLRLGRREGDMTFAPAGAQAPVHEESLGDGYLGDVPKPL
                     NLPFDPIERAAQRWEQTWGSASAMRVATSIMRAQQILLARYDELLKPHGLTFARYEVL
                     VLLRFSRYGALPLGRIGERLMVHPTSVTNSIDRLERAGFVERRPNPRDGRGTLAAITE
                     SGLAVVEAATSDLMAADFGLECLGEKSRDDVVAGLTRLRQDAGDFTG"
     gene            complement(892..>1170)
                     /locus_tag="VFU90_02435"
     CDS             complement(892..>1170)
                     /locus_tag="VFU90_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478656.1"
                     /translation="LGPMAVIELHVTAELPHSAGNETMTDRLGFDLAWTLSGTQSKST
                     AIVRGVPFVGGGAHPPYGKLAAIPSFVGALGLAAGWRRRVRGVGLHRR"
BASE COUNT          187 a          394 c          409 g          180 t
ORIGIN      
        1 tcgaacaacg tgcgcaacgc gttctcgctg tgcgcgtagc gcagcaccgc ccagctgccg
       61 agccccatcg cgacgccccg caagagtcgc tcggcggcga gcagtcgcag cacgaaggcg
      121 tcgcgcaagg cgcggccgcg cagcacgatc ggggcgtctt ccgccgggcc gctgcccttc
      181 ggctcgccga gcacgaacgt gccgcaccgc aggcaacgcc acgcctcgcc gaccggggtc
      241 ttcgtgtgca agcggtcgcg cagctccggc tcggacggcg cgaaggtgac atgacctttg
      301 cgcccgcagg cgcgcaggct ccagtccacg aggaaagcct aggcgacggg taccttgggg
      361 atgtgccgaa accgctgaac ttgccgttcg atccgatcga gcgggcggcg caacggtggg
      421 agcagacctg gggttcggcg tctgcgatgc gagtcgccac gtcgatcatg cgtgcgcagc
      481 agatcttgct ggcccggtac gacgagctgt tgaagccgca tgggctgacg ttcgcccgtt
      541 acgaggtctt ggtgttgttg cggttcagcc gctacggcgc gctgccgctt gggcgcatcg
      601 gcgaacggct catggtgcat ccgacgagtg tcacgaactc gatcgaccgg ctcgagcggg
      661 ccggtttcgt cgagcgccgg ccgaatccgc gcgacggccg tggcaccctg gcggccatca
      721 ccgagtcagg tctcgctgtc gtggaggccg cgacgtcaga tctgatggcg gccgactttg
      781 ggcttgagtg cctcggcgaa aaatcgcggg acgacgtggt cgccgggctc acccggctgc
      841 gccaagacgc gggcgacttc accggctgac gcgcacgcgt acgcgcaggg tctaacggcg
      901 gtgcagaccc acaccgcgga cgcgtcgtcg ccagccggcg gccaagccaa gcgcaccgac
      961 aaacgagggg atcgccgcca gttttccgta cgggggatgg gcgccgcctc cgacgaacgg
     1021 cacacctctg acgatcgcgg tgctcttcga ttgcgtgccg gacaacgtcc aggccaggtc
     1081 gaatccgagc cgatccgtca tcgtctcatt gcctgctgaa tgcggcagtt ccgcagtcac
     1141 gtggagttcg atcacggcca tggggccgag
//
LOCUS       DASUCJ010000196         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_735666, whole genome shotgun sequence.
ACCESSION   DASUCJ010000196 DASUCJ010000000
VERSION     DASUCJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735666"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFU90_02440"
     CDS             <1..462
                     /locus_tag="VFU90_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEU5478657.1"
                     /translation="AIANPGEQVAAVRAVAAAEGSRVVVLSGKVESELAGLTEAEAEA
                     FRHELGLERSGLDDLAAQAYDLLGLMTFLTAGEKEVRAWTIPRGTTAPEAAGKIHGDI
                     ERGFIRAEIVSYDDYVKYRTMDALKAAGLVRSEGRDYVMQEGDVVNFRFNV"
     gene            514..1773
                     /locus_tag="VFU90_02445"
     CDS             514..1773
                     /locus_tag="VFU90_02445"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5478658.1"
                     /translation="MTTPAREVSVFGDAIAYFKEAADLLELDGGMRRILTSPARQIIF
                     AIPFQRDSGEFEVYTGYRVQFSFARGPAKGGIRYHPGVTLDEVTALAFWMTWKCAVVD
                     LPFGGGKGGVTCDPSTLSMNEIERITRRYAAELVEVVGPDKDVPAPDVNTTPQIMAWF
                     MDTYSMHVRQNQPGVVTGKPLEIGGSKGRVEATGRGVTICALDEMSEMGLRPEAATVA
                     IQGFGNVGRYAAKLFEERGCKVVGISDVTGAYYNEQGIYVSGAMAHVAAHGTLEGFQG
                     GDKITNAELLASDCDVLVPAALEKVFTSETAPRVRAKLIVEGANGPTTPEADRIFKEK
                     GIVVIPDIMANAGGVTVSYFEWVQDRMGFFWKESEVNARLEEFLLANLHEIRAIGNSR
                     GATLRTAAYTLAIDRVVKALKMRGIYA"
     gene            complement(1786..2106)
                     /locus_tag="VFU90_02450"
     CDS             complement(1786..2106)
                     /locus_tag="VFU90_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5478659.1"
                     /translation="MARERITPEELDAAGAAAVLRIDVRKNPDDRQIPGSIRRDGTAL
                     ESADNPPFTPDQRVVLYCGSGNSSSRIAAALRERGYDATALEGGYRAWIEAGLPTEPR
                     PAPS"
     gene            complement(2111..>3393)
                     /locus_tag="VFU90_02455"
     CDS             complement(2111..>3393)
                     /locus_tag="VFU90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5478660.1"
                     /translation="TAPIGFEKPGNACKDYNGFNAAGYTLRGNRRTRMNPASTVIRNV
                     AVPGITLHEANTLTQPQSMTCEPLPGIQGLLAQVVDGESSTYWPVLGNWSFMGQRLTM
                     VNAAAQLKPTLATVWLGANDVLKYMGSGGLFHGGDTSAGQAAADVRQAVGTLQQAGAR
                     VVVANLPSILQTPYFMRVTIPQKPAQACAIQTYAACVIGLFFGYGGYPAAVAITRQVA
                     KRYHLATPAGCTPASTGNPCGYLTLQGTLAILQYYQIYGKVPDLDNGHPGSGMGTYYI
                     TPAFAGKIQTLNDALNQGIASAAHAKHSPLVDVHAIFEGIASGNPANPYFRQAVSIGT
                     NPSYPCCTLAYLAGLVSFDGLHPSNTGYALLAYSFIKAINGSFGQHIPQIDIQAAYTG
                     TRCSNPEQCYPDEYAPPYNQGGSLRPRGQYALQP"
BASE COUNT          579 a         1051 c         1165 g          598 t
ORIGIN      
        1 gcgatcgcga accccggcga gcaagtggcc gccgtgcgcg ccgtcgcggc cgccgaggga
       61 agccgcgtgg tcgtgctctc cggcaaggtc gagtccgagc ttgccgggct gacggaagcg
      121 gaggcggagg cgtttcgcca cgagctgggg ctggaacgca gcgggctgga cgacctggcc
      181 gcccaggcct acgacttgct cggcttaatg acgttcctga ccgccggcga gaaggaggtg
      241 cgcgcctgga cgattccgcg cggcaccacg gcgcccgagg cggccggcaa aattcacggc
      301 gacatcgagc gcggcttcat tcgagcggag atcgtgtcgt acgacgacta cgtcaaatat
      361 cgtaccatgg acgcgctcaa ggccgccgga ctcgtgcgaa gcgaaggccg cgattacgtc
      421 atgcaggagg gcgacgtcgt caatttccgc ttcaacgtct agcgagcggc tcgcgcggag
      481 gggtcgggcg agttggagcc caaactccac cgtatgacga ccccggcgcg tgaagtttcg
      541 gtttttggcg acgcgatcgc ctatttcaag gaagcggccg acttgctcga gctcgacggc
      601 ggaatgcgcc ggattcttac gagcccggcg cggcagatca tctttgcgat tccgtttcaa
      661 cgcgattcgg gcgagttcga ggtctacacc ggctatcgcg tgcagttcag cttcgcgcgc
      721 ggaccggcca agggcgggat ccgctatcat cccggcgtga ccctcgacga ggtcaccgcg
      781 ctggcatttt ggatgacgtg gaagtgcgcg gtcgtcgatc ttccgttcgg cggcggaaaa
      841 ggcggcgtaa cctgcgatcc gagcacgctg tcgatgaacg agatcgagcg gatcacgcgc
      901 cgttatgccg ccgagctggt cgaggtggtt ggtcccgata aggacgttcc cgcgcccgac
      961 gtcaacacga ccccgcaaat catggcgtgg ttcatggaca cgtactcgat gcacgtgcgc
     1021 cagaaccagc ccggggtcgt caccggcaag ccgctcgaaa tcggcggctc gaaaggccgc
     1081 gtcgaagcca ccggccgcgg cgtgaccatt tgcgcgctcg acgagatgag cgagatgggc
     1141 ttgcgccccg aagcggcgac cgtcgccatc cagggtttcg gcaacgtggg acgctacgcc
     1201 gccaagctgt tcgaagagcg cggctgcaag gtggtcggca tctccgacgt caccggcgcc
     1261 tactacaacg agcaggggat ctacgtctcg ggcgcgatgg cgcacgtcgc cgcgcacgga
     1321 acgctcgagg gcttccaagg cggcgacaag attaccaacg ccgagctgct ggccagcgat
     1381 tgcgacgtgc tcgtaccggc agcgctcgaa aaagtgttca ccagcgaaac ggccccgcgc
     1441 gtgcgcgcca agctgatcgt cgaaggcgcc aacggtccga ccactcccga agccgaccgg
     1501 attttcaagg agaagggcat cgtcgtcatc cccgacatca tggccaacgc cggcggcgta
     1561 acggtttcgt atttcgagtg ggtgcaagac cggatgggat tcttttggaa ggagtccgag
     1621 gtcaacgcac ggctcgaaga gtttctgctc gccaacttgc acgaaatccg cgcgatcgga
     1681 aactcgcgcg gcgcgacgct gcgtaccgcg gcctatacgc tcgcgatcga tcgcgtcgtg
     1741 aaggcgctca agatgcgcgg catctacgcc taacccggcg tccgtctacg aaggagcggg
     1801 ccgcggctcg gtgggcaagc cggcctcgat ccaggcgcgg tagccgcctt cgagggcggt
     1861 ggcgtcgtag ccccgctcgc gcagtgcggc ggcgatccgg ctcgaggagt tgccgctccc
     1921 gcaatacagc acgacccgct gatccggcgt aaacggcgga ttgtcggcgc tttccaacgc
     1981 cgtaccgtcg cgccggatcg atcccggaat ttgccggtcg tcgggattct tgcgaacgtc
     2041 gatgcgcagc acggcggccg caccggcggc gtcgagctct tcgggggtta tgcgttcgcg
     2101 cgccacggcg tcacggctga agagcgtact gcccgcgcgg ccgcaaggaa cccccttggt
     2161 tgtacggggg ggcgtattcg tcgggatagc attgctcggg gttcgagcag cgcgtgcccg
     2221 tgtacgcggc ctgaatgtcg atttgcggga tgtgctgtcc gaacgatccg ttgatcgcct
     2281 tgatgaagga gtacgcgagc aaggcgtaac cggtgttcga cggatgcagc ccgtcgaagc
     2341 ttaccagccc ggctaagtac gcgagcgtgc agcacggata gctcggattg gttccgatcg
     2401 agaccgcctg gcggaagtag ggattggccg ggtttccgct cgcgatccct tcgaagatcg
     2461 cgtgcacgtc gaccagcggc gaatgcttcg cgtgcgcggc gctggcgatg ccctggttga
     2521 gcgcgtcgtt gagcgtttga atttttccgg cgaatgctgg cgtgatgtag tacgtgccca
     2581 ttccgctgcc gggatgcccg ttgtcgagat ccggcacctt gccgtagatt tggtagtact
     2641 gcagtatcgc cagcgtgcct tgcagcgtca ggtagccgca cggatttccg gtcgaggcgg
     2701 gcgtgcatcc ggccggcgtc gcgaggtgat aacgcttcgc gacttgccgc gtgatagcaa
     2761 cggccgccgg gtagcctccg tacccgaaga acaaaccgat cacgcacgcc gcgtaggttt
     2821 gaatcgcgca cgcttgagcg ggtttttggg gaatcgtcac gcgcatgaag tacggcgtct
     2881 gcagaatgct cgggagattg gccaccacca cgcgcgcgcc ggcttgctga agcgtgccga
     2941 cggcttggcg aacgtcggcg gccgcttgac cggcgctcgt gtcgccgccg tggaagagcc
     3001 cgcccgagcc catgtacttg agcacgtcgt tggctccgag ccaaacggtg gccagcgtcg
     3061 gtttgagctg cgccgcggcg ttgaccatcg tgagccgctg ccccataaac gaccagttgc
     3121 cgagcacggg ccagtaggtg ctgctttcgc cgtcgacgac ttgcgcgagc aagccttgga
     3181 tgccgggaag cggttcgcag gtcatcgact gcggctgcgt cagcgtattg gcttcgtgca
     3241 gcgtgatgcc gggcaccgcg acgttgcgaa ttaccgtcga cgccgggttc atccgggtgc
     3301 gccggttgcc gcgcagcgtg taaccggcgg cgttgaatcc gttgtaatcc ttgcacgcat
     3361 tgccgggctt ctcgaaaccg atcggcgcgg tga
//
LOCUS       DASUCJ010000197         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1377437, whole genome shotgun sequence.
ACCESSION   DASUCJ010000197 DASUCJ010000000
VERSION     DASUCJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377437"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU90_02460"
     CDS             <1..198
                     /locus_tag="VFU90_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5478661.1"
                     /translation="EVFLREPRRVFSKDHLLEIVWGHDFEGGPNIVETYISYLRAKID
                     RPGGPGSFIRTVRGVGYGLSQ"
     gene            205..1593
                     /locus_tag="VFU90_02465"
     CDS             205..1593
                     /locus_tag="VFU90_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5478662.1"
                     /translation="MKLASRLSAIYAGLLGVTVLIVILASSISLVEELYGFTGDIVIA
                     KHEEARILADRYHREGITLAQAAPEIVAALGGIGLRVTVFDAKGHFLAGDKTLRPKVL
                     DRILALGGIEHFIPHPPPRPPLGQPPPDPARLDPLSLAPVQGGYVAFVPSRPLLVRSL
                     VPYWRIVLTIAIGAMFFSFLFGRWFAAQALKPIDEVSKSLHALASGDYTQRRFIMQGG
                     DEVASLTEAFNDAAANVASAMDDRRRAEDRMRQFAADASHELRTPLTVIGGYIDVLRR
                     GAIEEPKVARQILSTMSIEKEHMRSLIDRLMRLARLDSEAMPRVEKIDIGDFLSGQCE
                     AARRLDDRRVIDYSVEGVQTIEADRSELGEAIWNIVENALKYAPDAPIHLSARGDDGH
                     AVITVRDEGPGMSESERVHAFERFYRGDERGEIAGTGLGLAIAKRAVERAGGGIAIDS
                     APGHGTAVTITL"
     gene            1671..>3149
                     /locus_tag="VFU90_02470"
     CDS             1671..>3149
                     /locus_tag="VFU90_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478663.1"
                     /translation="MRRFVTAVVAAVLALVDPATAFAQTDSGEIRIAVTDAASKQPIG
                     LARVLLDGAVITSELTSSDGKVTFTEVPDGIYRARVVKRGYQGLTSSEFEVVNGRIVT
                     VDVALVAETGGLKVIGTVSVKASATISSTSIDQNSPQRRLSDDLAGALNKLSGVSVTT
                     SGDDSDATQTISLEGHDASQTALTLDGIPLNAPGSAANLRGFATDLFQGASVRTGAQL
                     GGLGGSVNFTTLQPTLSWESQIAASMGSNAKYNYSFAESGSLGKLGLAVQTVYRSVPS
                     LVDGDFYLDASGLAYDHNGDSSYSGNLLKLRYDIGDSQAITGTFLNSARDTNITCLRQ
                     GAPPALPCGYGPGNTNDSSVQLYALTDNALLGATQVQASLYSSTATNVLDEIDRSIDV
                     STGPLPEPSSAPNGFSTHGRTNGFSVNATLPAQQRHTISIQAYGSGSQNVTSPLVPQA
                     IPYYTGAFNTAYDAVSVTDTMHSSDKLTLAESLGVSTATGSGG"
BASE COUNT          574 a         1034 c          979 g          562 t
ORIGIN      
        1 gaggtgttct tgcgcgagcc gcggcgggtc tttagcaagg atcacctgct ggagatcgtc
       61 tggggtcacg acttcgaggg tgggccgaac atcgtcgaaa cgtacatctc ttacctgcgt
      121 gcgaagatcg accggcccgg aggacccggc tccttcattc gcaccgtgcg gggcgtcggc
      181 tacggcctct cccaataggg ctcgatgaag ctcgcctcac gtctgtccgc aatctatgcg
      241 ggtttgttgg gcgtgaccgt gctcatcgtg atcctggcgt cttcaatttc gctggtcgaa
      301 gagctttacg ggtttaccgg cgacatcgtc attgcgaaac acgaagaagc ccgcatcctc
      361 gccgaccggt accaccgcga aggcataacg ctcgcgcaag cggcgcccga gatcgtcgcc
      421 gcgcttggcg gcatcggatt gcgggtgacc gtcttcgatg cgaaggggca cttcctcgcc
      481 ggcgataaga cgctgcggcc gaaggtgctc gaccgcatcc tggcgctcgg cggaatcgag
      541 catttcattc cgcacccgcc gcccaggccg ccgctggggc agccgccgcc cgatccggcc
      601 cgcttggatc cgctttcgct cgcgccggtt caaggcggat acgtggcgtt cgtgccgtcg
      661 cgacctttgc tcgttcgttc gctcgtgccg tactggcgca tcgtcttgac gatcgcgatc
      721 ggcgcgatgt tcttttcgtt cctgttcgga cgctggttcg ccgcgcaggc gctcaagccg
      781 atcgacgaag tcagcaagtc gctgcacgcc cttgcgagcg gtgactacac gcaacggcga
      841 ttcatcatgc aggggggcga cgaggttgcc tcgttgaccg aagcgttcaa cgacgcggcc
      901 gcgaacgtcg cctcggcaat ggacgaccgc cgtcgcgcgg aagaccggat gcgccaattt
      961 gcggccgatg cctcacacga gctgcgaacg ccgctgacgg tgatcggcgg atacatcgac
     1021 gtcctgcgtc gcggcgcgat cgaagagccg aaggtcgcgc ggcagatcct ctcgacgatg
     1081 tcgatcgaga aggaacacat gcgcagcttg atcgatcgcc tcatgcggct ggcgcggctg
     1141 gactccgagg cgatgccgcg cgtcgagaag atcgatatcg gagacttcct cagcgggcag
     1201 tgcgaagccg ctcggcgctt ggacgaccgg cgcgtcatcg actatagcgt cgaaggcgtc
     1261 cagacgatcg aggccgaccg cagcgaactg ggcgaagcca tttggaacat cgtcgagaac
     1321 gcgctaaagt atgccccgga tgccccaatc cacctgagcg cccggggcga cgacgggcat
     1381 gccgtgatta cggtccgtga cgagggtccg ggaatgtcgg aatcggaacg cgttcacgcc
     1441 ttcgaacgct tctatcgtgg tgacgaacgc ggtgagatcg ccggcaccgg cttgggacta
     1501 gccattgcaa agcgcgcggt ggaacgcgcc ggcggcggta tcgcgatcga cagcgctccg
     1561 ggccacggga ccgccgtcac catcaccctc taggcctgta ttccgcagcc ctcggcgcgg
     1621 cgttttcaca gccccgcttc aggcgtgctc gcgtacaatc tccatcggtt atgcgacggt
     1681 tcgttaccgc tgtcgtcgcg gcggtgctgg cattggtcga tcctgcaact gccttcgcgc
     1741 agacggattc gggtgagatt cgtattgcgg taacggatgc ggcgagcaag cagccgatcg
     1801 ggctcgcgcg cgtcctgctc gacggtgcgg tcatcacgtc ggaacttacc agttccgacg
     1861 gcaaggtcac tttcaccgag gttcctgacg gaatctaccg cgcacgcgtc gtcaagcgcg
     1921 gctatcaagg cctgacgtcg tcggagtttg aggtcgtcaa tgggcgcatc gtcaccgtgg
     1981 acgtcgcgct ggtggccgaa acgggcggcc tcaaggtcat cggtaccgtc agtgtcaagg
     2041 catcggcgac catctcctcg accagcatcg atcaaaactc cccgcaacgc cgtctctccg
     2101 acgacttggc cggcgcgctc aacaaactgt cgggcgtaag cgtcacgacc agcggcgacg
     2161 acagcgacgc gacccagacg atctcgctgg aaggtcacga tgccagtcaa accgccctca
     2221 cgctggacgg catcccgttg aacgcgcccg gcagcgccgc aaacctgcgc ggcttcgcga
     2281 ccgacctgtt ccagggcgcc tcggttcgta ctggagcgca actgggcggt ttgggcggct
     2341 cggtcaactt cacgaccctc cagccgaccc tcagctggga atcgcaaatc gccgcctcga
     2401 tgggaagcaa cgcgaagtac aattactcgt ttgcggaaag cggttcgttg gggaagctcg
     2461 gtctcgccgt ccagaccgtc tatcgttcgg taccgagtct ggtcgacggc gacttctacc
     2521 tggacgccag cggcctcgcc tacgatcata acggcgacag cagctactcc ggaaacctgc
     2581 tgaagctgcg ctacgatatc ggcgacagcc aagcgataac cgggacgttt ctcaactcgg
     2641 cgcgcgacac caatatcacg tgtttgcgcc agggagcgcc accggcgctg ccctgcggat
     2701 acggccccgg caacaccaac gacagcagcg ttcagctgta tgcgctcacg gacaacgcgt
     2761 tgctaggtgc gacgcaggta caggcatcgc tgtactcgtc gacggcaacc aacgtgctgg
     2821 atgagatcga tcgctccatc gacgtctcga ccggaccgct ccccgagccg agtagcgcgc
     2881 cgaacggttt ctcgacacat ggccgaacga atgggttctc ggtaaatgcc acgctgccgg
     2941 cccagcaacg gcacacgatc tcgatccaag cctatggctc gggctcccaa aacgttacgt
     3001 ccccgctggt cccgcaggcg atcccatact acaccggcgc cttcaatacg gcctacgacg
     3061 ccgtcagcgt gaccgacacc atgcattcaa gcgacaaact cactctcgcc gaatcgctcg
     3121 gcgttagcac ggccaccggc tcgggcggc
//
LOCUS       DASUCJ010000198         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_552256, whole genome shotgun sequence.
ACCESSION   DASUCJ010000198 DASUCJ010000000
VERSION     DASUCJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552256"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1306
                     /locus_tag="VFU90_02475"
     CDS             101..1306
                     /locus_tag="VFU90_02475"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEU5478664.1"
                     /translation="MKVLVLNCGSSSVKLQLVDAEAGAADHAPLAVRARGAVHNVSSP
                     NATWSVDVGDRHVDGELHLRDHSSAARELLARFRDAYGGAIDAVGHRIVHGGSRYREA
                     TVIDRAVIDSLTELEELAPLHNGPCVAGVRAARAALGDAVPMTASFDTTFHRDLPPVA
                     SQYAIPAGIAERCGIRRYGFHGLSYQSVLARYCHLTDTPSGQATLIVLHLGSGCSAAA
                     IRDGRSADTSMGFTPLEGLMMSTRSGDLDPAIVAFLAQHDHVSATTVEQWLNTRSGLL
                     GVSRSTADMRELLAVERDDEHARLAIEMFAYRARKYVGAYLAALGGAAAVVFTGGIGE
                     NSPVVRTRILDGMEWLGIRLDPACNSSCVGAPARLSTDDSRVAVYSIPTDEEGVIARD
                     AVRLLNTPA"
     gene            1513..1692
                     /locus_tag="VFU90_02480"
     CDS             1513..1692
                     /locus_tag="VFU90_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478665.1"
                     /translation="MSRSRDRILSNLDEMYREAFERAKSTQDQAQMSTLDFAYRREQL
                     YFEILLDIRDAMERR"
     gene            1693..3372
                     /locus_tag="VFU90_02485"
     CDS             1693..3372
                     /locus_tag="VFU90_02485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5478666.1"
                     /translation="MRAPLIALALLALLVTTPPHTASSPVTMVVVNARVWTGDSTRPW
                     ADAIAVSGDTLAAVGSSAEVRKMAAPDTRIIDAHGAMLVPGFIDAHVHFLDGGFALSS
                     VQLRDAKTPAEFVRRIEAYAATLPKGAWILGGDWDHTLWGGELPRRDWIDSVTGDHPV
                     WVGRLDGHMGLANTAALRAAGVSRATPDVDGGTIVRDASGEPTGVLKDNAMGLVTKAI
                     PDAPPEVEDRAFDAAMAYVVARGVTQVDHMGTWHDLAVLERAHKAGRMKARISAAVPL
                     ATWERLRDTIAARGRGDAWLHIGGLKGFVDGSLGSHTAAMLAPFTDAPKDSGLLVNTP
                     EDLYAWTSGADKAGLQVICHAIGDRAIRLQLDIYQRVEQEDGPRDRRFRIEHAQHVAP
                     ADIPRFAQLGVIASMQPYHAIDDGRWAEAVIGHERSKTTYAFRSLLDAGTRLAFGSDW
                     DVAPPTAIDGIYAAVTRRTLDGKNPNGWIPEQKITVEEALRAYTIGAAYASFDEKRKG
                     SLVKGKLADFVIIDRDLTKIPVEQIRDAEVVMTVVGGTVVYTRHADDSRRS"
     gene            complement(3444..4307)
                     /locus_tag="VFU90_02490"
     CDS             complement(3444..4307)
                     /locus_tag="VFU90_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478667.1"
                     /translation="MRGLFMLAAALSAVGSMAHGQVATTASGHHAAPSPDSTITIPAG
                     VRIFAQLQRPLSTRTAKPGDSIYMQVTVPVTVGNAVVIPAGTYLLATLDTVQRIGWIH
                     RSVAFQLRVASMVFSNGYVATVAEPAQAEPRDPSALGPGERPAALMLASGAAPVAGLA
                     IGAMAGGKNGAWLGSEVGGAVGLVTFIVAAAHSGEYQLGPGFPLALRSQAPMTLDAAR
                     IADAARTAATVQHHPNKSCYTPGTLGTPDTFIPGTPGTPPMGDMPGTPDTPPTIIPGT
                     PGTPGYWHPCP"
     gene            complement(4671..>4885)
                     /locus_tag="VFU90_02495"
     CDS             complement(4671..>4885)
                     /locus_tag="VFU90_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5478668.1"
                     /translation="DPNAHHHAGTTRMHDDPALGVVDRNARVHGMDNVYAAGASIFPT
                     AGFANPMLTIVALTVRLARHLLDESR"
BASE COUNT          778 a         1563 c         1751 g          793 t
ORIGIN      
        1 cgagcatctg ctgggcggcc gcgtcgtgga agcgtaccgg catcgtgcgc cgccgggcga
       61 caacaagctc gtggactgac gcgatgggag ctcgttcggc gtgaaggtgc tggtgttgaa
      121 ttgcggcagc tcgtcggtca agctgcagct cgtggacgcc gaggccggcg cggccgacca
      181 cgcgccgctc gccgtgcggg cgcgcggcgc ggtgcacaac gtgtcatcgc ctaacgcaac
      241 gtggtcggtg gacgtgggcg accggcacgt cgacggcgag ctgcacctgc gcgatcactc
      301 gtccgccgcg cgggagctgc tggcgcggtt ccgggacgcg tacggcggcg cgatcgacgc
      361 cgttgggcat cgcatcgtgc acggcgggtc gcggtaccgc gaggcgacgg tcatcgaccg
      421 ggccgtcatc gactcgctca ccgagctcga ggagctggcg ccgctgcaca acggaccgtg
      481 cgtggccggg gtgcgggccg cgcgcgctgc gttaggcgat gccgtgccga tgacggcgtc
      541 gttcgacacg acgtttcatc gcgacctgcc gccggtcgcg tcgcagtacg ccattcccgc
      601 cggcatcgcc gagcggtgcg gcatccgtcg ctacggcttt cacggcctgt cgtaccagag
      661 cgtgctcgcg cggtattgcc acctcaccga cacgccgtcc gggcaggcga cactcattgt
      721 actacacctc ggcagcggct gctcggccgc cgccatccgc gacggtcgtt cggcggacac
      781 gagcatgggt ttcacgccgc tcgagggctt gatgatgagc acgcgcagcg gcgacctcga
      841 tcctgccatc gtcgcgtttc tcgcccaaca cgaccacgtc agcgcgacga cggtagagca
      901 gtggctcaat acccggtcgg gcctgctcgg cgtgtcgcgc tcgacggcgg acatgcgcga
      961 gctgttggcg gtcgagcgag acgacgagca cgcacggttg gccatcgaga tgttcgcgta
     1021 tcgtgcgcgc aaatacgtgg gcgcgtatct cgccgcgttg ggcggcgccg cggcggtcgt
     1081 gttcaccggt ggaatcggcg aaaactcgcc ggtggtgcga acgcgcatcc tcgatgggat
     1141 ggaatggctc ggcatccgac tggatccggc gtgcaacagt tcgtgtgtcg gcgcgccggc
     1201 tcgcctgagc actgacgatt cgcgcgtggc ggtatactcc atcccgaccg acgaagaagg
     1261 tgtgatcgcg cgcgacgcgg tacggctgct gaacacgcct gcgtgaggcg agctcgagca
     1321 ttggcggcag cctcgcgatc ggctatagtg tgtgagacct gaactcctgt gtcgttcgaa
     1381 cccattcctc cacgccgtgc tctcatgggt gcggccaagc gattccttct cgcacgagcc
     1441 gaagcgagca tcaagccgcc ggccgagatc gagccgcccc gtctcatcct tcgagccctc
     1501 gaggcaatcc gtatgtcccg ttcgcgcgat cgcatcctgt ccaacctcga cgagatgtac
     1561 cgcgaggcgt tcgagcgtgc caagtcgacg caagatcagg cgcagatgtc gacgctcgat
     1621 ttcgcctatc ggcgcgagca gttgtacttc gagattctgt tggacattcg cgacgcgatg
     1681 gagcgtcgct gagtgcgcgc gccgttgatc gcgctcgcgc tcctggccct gctcgtgacc
     1741 acgcccccgc acaccgcatc gtcgccggtg acgatggtcg tcgtgaacgc gcgcgtgtgg
     1801 acgggcgact cgacgcgtcc ctgggcggac gcgatcgcgg tgagcggcga cacgctcgcc
     1861 gcggtgggct ccagcgccga agtacggaag atggccgcac cggacacgcg catcatcgac
     1921 gcgcacggcg cgatgctggt gccgggattc atcgacgccc acgtgcattt cctcgacggc
     1981 ggcttcgcgc tgtcgtcggt gcagctgcgc gacgcgaaga cgcccgcgga attcgttagg
     2041 cgcatcgagg cgtacgccgc gacgctgccc aaaggcgctt ggatactcgg cggcgattgg
     2101 gatcacacct tgtggggcgg cgagctgccg cggcgcgact ggatcgactc agtgacgggc
     2161 gaccatccgg tatgggtcgg tcgtctcgat gggcacatgg gtctggcgaa caccgctgcc
     2221 ttacgcgccg cgggcgtgag ccgtgcgacg ccggatgtgg atggcggcac catcgtgcgc
     2281 gacgcgagcg gcgagcccac cggcgtgctc aaggacaacg cgatgggcct cgtgacgaag
     2341 gcgatccccg atgcgccgcc cgaggtggaa gatcgcgcgt tcgatgcggc gatggcctac
     2401 gtggtcgcgc gaggcgtgac gcaggtcgat cacatgggga cgtggcacga tctggcggta
     2461 ctcgagcgcg cgcacaaggc gggccggatg aaagcgcgca tctcggcggc ggttccgtta
     2521 gccacgtggg agcggttgcg cgacacgatt gccgcgcgcg gccgcgggga cgcgtggctg
     2581 cacatcggcg ggctcaaggg attcgtggat gggtcgttag gctcgcacac ggcggcaatg
     2641 ctcgcgccgt tcaccgatgc gccgaaggac agcgggctgc tcgtgaacac gcccgaagac
     2701 ctgtatgcgt ggacgagcgg cgccgacaag gccggcttgc aggtgatctg ccacgcgatc
     2761 ggggaccggg cgatccggtt gcagctggac atctaccagc gcgtggagca ggaagacggc
     2821 ccgcgcgacc ggcgcttccg gatcgagcac gcgcagcacg tggcgccggc ggacatcccg
     2881 cggttcgccc agttaggcgt gatcgcgagc atgcagccgt atcacgcgat cgatgatggt
     2941 cgttgggcag aggccgtgat cgggcacgag cggagcaaga cgacctacgc gttccggtcg
     3001 ttgctcgatg cggggacgcg cctcgcattc gggtcggatt gggacgtggc gccgccgacg
     3061 gcaatcgacg ggatttacgc ggcggtcacg cgacgcacgc tcgacggcaa gaatcccaac
     3121 ggatggattc cggaacagaa aatcacggtg gaagaagcgc tgcgcgcgta cacgattgga
     3181 gcggcgtacg cgtcgttcga tgaaaagcga aaggggtcgt tggtgaaggg caagctggcg
     3241 gacttcgtga tcatcgaccg ggatctcacg aagattccgg tcgagcagat tcgggatgca
     3301 gaagtcgtga tgactgtggt cggagggacg gtggtgtata cgcggcacgc cgatgactct
     3361 cggcggtcgt aggctcttgc gctcggagtg atcggactcg agcatcccga acgcacgtgg
     3421 cctggcctaa cggaacgacg gtgtcacgga cacggatgcc agtagcccgg cgttcccggg
     3481 gtgcccggga tgatcgtggg cggcgtgtcc ggcgtgcccg gcatgtcgcc catcgggggc
     3541 gtccccggcg ttcccggaat gaacgtgtcg ggcgtgccga gcgtgcccgg cgtgtagcaa
     3601 ctcttgttcg ggtgatgctg taccgtcgcc gcggtccgcg cggcgtccgc gatgcgcgcg
     3661 gcgtcgagcg tcatcggagc ctgggaacgc agtgccaacg ggaatccggg gcccagctgg
     3721 tactcgccgc tgtgcgccgc ggccacgatg aaggtcacga ggccgacggc gcccccgacc
     3781 tcgctgccta accaggcgcc gttcttgccg ccggccatcg ccccgatcgc gagacccgcc
     3841 accggcgcgg cgccgctggc gagcatcaac gcagccgggc gctcgccggg gcctaacgcg
     3901 ctcgggtcgc gtggctccgc ctgcgcgggc tcggccacgg tcgcgacata gccgttcgag
     3961 aacaccatcg acgccacgcg gagctggaac gccacactgc gatggatcca gccgatgcgc
     4021 tgaaccgtgt cgagcgtggc cagcaggtag gtcccggcgg gaatcaccac ggcatttccg
     4081 accgtcaccg ggactgtcac ctgcatgtag atgctgtctc ccggcttggc cgttcgcgta
     4141 gacaacggcc gctgcaactg tgcgaagatg cgcacccccg ccggtatcgt aatggtcgag
     4201 tcagggctcg gcgcggcgtg atggccgctc gcggtggtcg cgacctgacc gtgtgccatc
     4261 gagccgaccg ccgatagtgc cgctgcgagc atgaagagtc cacgcatagt ccctccctcg
     4321 ttcggatgcc ctgtcacaca cagccttgtc ttaggaagac ggcgtgcaca gccggagttc
     4381 cgagagccgt gcggtgggtc gctggcacac gtcggtagtg cattcaccac ggcgtcttgc
     4441 gcatcgtgcg gagcgctcgc gattgattcg gcgatcatca gaacggcgta cggctggttc
     4501 gcggtcgccg gcatccagcg cacgcctaac gaactgcgat tcgcgatgga cacggcgcag
     4561 cgatcgggcg cgcgggcgta ccatcaccgg cccatggacg acaacactga tcgacgaacg
     4621 accttctcgg acgtgatgca cctgttcgac gcggcgctga acgcaatgcg tcagcgggac
     4681 tcgtcgagga ggtgcctggc caatcggacg gtgagtgcga cgatcgtgag catcgggttc
     4741 gcgaaaccgg cggtcgggaa aatcgatgcg ccggcggcgt agacgttgtc catgccatgc
     4801 acgcgcgcgt tgcgatcgac cacgccgagt gcgggatcgt cgtgcatgcg cgtcgtgccg
     4861 gcatggtgat gcgcgttggg atcgg
//
LOCUS       DASUCJ010000199         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1377473, whole genome shotgun sequence.
ACCESSION   DASUCJ010000199 DASUCJ010000000
VERSION     DASUCJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377473"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFU90_02500"
     CDS             complement(<1..1181)
                     /locus_tag="VFU90_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU5478669.1"
                     /translation="MIVRRYLKPIAWTIAAGALGLLIVLWLRPAPIGVDVGTVARRRV
                     EVAVESEAKTRIHDRFVVSAPVAGRLLRIPFEEGDRVAAGQTVASIDPLPYNASIASA
                     LQKLRELQAQRSGIETFRPKEEALEQARSRVTASLASARSTYARVLAAEAAYQQADRE
                     ASRESSLERQGYASKSAAEQAQLVRTTRLRELQMARTGAAAADAQFAVDRAIVEELTK
                     KVRDPDYLRTVYDAQMSAIRAQLRNLEDQAHRTVVTAPVSGDVLRVVQKSEAYVPSGA
                     PLLEIGSRRTLEIVADVLSQDAVRIRTGDRVEVIRGAGDTHPKAVVRRIEPSGFTKIS
                     ALGIEEQRVNVIAAFESPPPSLGDAYRLDVRIVTWSGDALAIPVPALVRCGVDWCAYV
                     V"
     gene            complement(1178..>1516)
                     /locus_tag="VFU90_02505"
     CDS             complement(1178..>1516)
                     /locus_tag="VFU90_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478670.1"
                     /translation="NSGRITFSERLRELGTLRILGYSRLDVTLVLAGEQLLLTLAAVP
                     VGFLIGHGLNRALQPLYELEYYRVPTVVSNQTYVFACAFVLFAAGLSAAALALQVRRL
                     DVVAALKAGE"
BASE COUNT          269 a          493 c          503 g          251 t
ORIGIN      
        1 tcgacgacgt acgcgcacca atcgactccg cagcgcacca gcgcgggaac cgggattgcg
       61 agcgcgtcgc ccgaccaggt aacgatacga acgtccagcc ggtaggcatc gcccagcgac
      121 ggcggcggac tttcaaaagc ggcgatgacg ttgacgcgct gttcctcgat gccgagcgcg
      181 gaaatcttcg tgaagccgga gggctcgatg cgccgaacga cagctttagg atgcgtgtcg
      241 ccggcgccgc gaattacctc gacgcgatcg ccggtccgga tgcggaccgc atcctgcgaa
      301 agaacgtcgg cgacgatttc gagggtgcgc cggctgccga tttccagcaa cggcgcgccg
      361 gagggtacgt aggcttcgct cttttggacg acgcgtagga cgtcgcccga aaccggagcg
      421 gtcacgacgg tgcggtgggc ttgatcttcc aagtttcgca gctgcgcccg gatcgcgctc
      481 atctgcgcgt cgtacacggt acgcaagtag tcggggtcgc gtaccttttt ggtcaactcc
      541 tcgacgatcg cgcgatcgac cgcgaattga gcgtcggccg cagccgcgcc ggttcgcgcc
      601 atttgcagtt cgcgaagccg cgtcgtgcgc acgagctgtg cctgttcggc cgccgacttc
      661 gatgcgtatc cctgacgttc gagcgagctc tcgcgactgg cctcgcgatc ggcttgctgg
      721 tacgccgctt cggccgcgag cacgcgggcg tatgtcgaac gcgccgaagc gaggcttgcg
      781 gtgacgcgcg aacgtgcttg ttcgagcgct tcctctttgg gccggaacgt ctcgattccg
      841 gaacgctgcg cttgaagttc gcggagcttc tgcagcgccg acgcgatcga cgcgttatac
      901 ggcagcggat cgatcgaggc gaccgtctga ccggcagcca cgcgatcgcc ttcctcgaag
      961 gggatgcgta gcagccgccc cgcgacgggc gccgaaacga cgaaccggtc gtggatgcgc
     1021 gttttagcct cggactctac ggcgacttcg acgcgccggc gggcgaccgt ccccacgtcg
     1081 acgccgatcg gcgccggccg cagccacagc acgatcagca acccgagcgc gccggcggcg
     1141 atcgtccaag cgatcggctt gaggtagcgc cgcacgatca ttctcccgcc ttcaaagcgg
     1201 cgacgacatc gagccggcgc acctgcagcg cgagggcagc ggccgacagg ccggcagcga
     1261 acaggacgaa cgcgcacgcg aatacgtacg tttggttgga gacgaccgtt gggactcggt
     1321 agtattcgag ctcgtacaac ggctgcagcg cccgattgag accgtgcccg atgagaaatc
     1381 ctaccggcac ggctgccaaa gtcaatagga gctgttcgcc ggctagcacg agcgtaacgt
     1441 ccaatcgcga gtagcccagg attcgcagcg tgccgagttc gcgcaagcgt tccgagaacg
     1501 ttatccgccc cgagtt
//
LOCUS       DASUCJ010000200         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_170108, whole genome shotgun sequence.
ACCESSION   DASUCJ010000200 DASUCJ010000000
VERSION     DASUCJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170108"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..843)
                     /locus_tag="VFU90_02510"
     CDS             complement(97..843)
                     /locus_tag="VFU90_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5478671.1"
                     /translation="MQISGNVALVTGAASGLGAATAARLRAAGAQVVLADLQPVDAGD
                     GDRYVRCDVTSEDDVLAATQAAADLGRFAMCVHCAGGGIAARTISRDGTPHALDAFRR
                     VIELNLVGTFNVLRLAASQMSHNEPDDNGERGACVLTASIAGYEGQIGQIAYGSAKGG
                     VIGMTIIAARDLSAAGVRVCTIAPGTFATPPMLMVPDAMREAFEANVPFPKRFGDPGE
                     YAALAQHIVENGYLNGEVIRLDGAVRFQPR"
     gene            complement(813..>1269)
                     /locus_tag="VFU90_02515"
     CDS             complement(813..>1269)
                     /locus_tag="VFU90_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478672.1"
                     /translation="PYNQHRYFTNYHVWETLVAWDAPEHYGVACKRVDARDASTKSPF
                     NERRRMPEALARVIRDVDAELVVVSYNNESWVTIDELEAMCAARGVEAVRSLAFDSRR
                     YVGAQIGIHNPNGEKVGTVSHLRNVEYVVCAGPAKMVEGLCRSQEMSRS"
BASE COUNT          209 a          425 c          425 g          210 t
ORIGIN      
        1 gatctcgcca cgctcatgaa caacgccccg ggctggtggg tcgagcacat catggccggc
       61 gcggccgaga tcgggctgca aggcgactga cgcgcgtcat cgcggctgga agcgcaccgc
      121 gccgtcgagg cggatgactt cgccgttgag atagccgttc tcgacgatgt gctgcgcgag
      181 cgcggcgtac tcgccggggt cgccgaagcg tttgggaaac gggacgttcg cttcgaacgc
      241 ttcgcgcatc gcgtcgggaa ccatgagcat cggcggcgtc gcgaacgtcc cgggcgcgat
      301 cgtgcacacg cgaacgccgg cggccgagag atctcgtgcg gcgatgatcg tcatgccgat
      361 gacgccgcct ttcgcagaac cgtacgcgat ctggccgatc tgaccctcgt accctgcgat
      421 cgatgccgtg aggacgcacg caccgcgctc accgttgtcg tccggttcgt tgtgcgacat
      481 ctgcgacgcg gccagacgga gcacgttgaa cgttccgacg agattgagct cgatcacgcg
      541 tcggaacgcg tcgagcgcgt gcggcgtgcc gtcacgcgag atcgtgcgcg ccgcgatgcc
      601 gccaccggcg cagtgcacgc acatcgcgaa ccgtccgagg tcggcggccg cttgcgtcgc
      661 ggcaagcacg tcgtcttcgg aggtgacgtc gcaacggaca taccggtcgc cgtcgccggc
      721 atcgaccggt tggaggtcgg ccaggacgac ctgcgcacct gcggcgcgca gccgcgctgc
      781 cgtcgcggca cccaatcctg acgcggcgcc tgttacgagc gcgacatttc ctgagatctg
      841 cacaacccct ccaccatctt tgcgggcccg gcgcacacga cgtattcgac gttgcgaaga
      901 tgagacaccg taccgacctt ctcaccgttc ggattatgga tgccgatctg cgcaccgaca
      961 taacgccgcg agtcgaacgc gagcgagcgc acggcttcga caccgcgtgc ggcgcacatc
     1021 gcctcgagct cgtcgatcgt cacccacgac tcgttgttgt acgagacgac gacgagctcg
     1081 gcgtcgacat cgcggatgac ccgcgcgagc gcttcgggca tgcgccggcg ttcgttgaac
     1141 gggctcttcg tcgacgcgtc acgggcgtcg acacgcttgc atgcaacgcc gtagtgctcg
     1201 ggcgcgtccc aagcgacgag cgtctcccac acgtggtagt tggtgaagta gcggtgctgg
     1261 ttgtagggc
//
LOCUS       DASUCJ010000201         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1117496, whole genome shotgun sequence.
ACCESSION   DASUCJ010000201 DASUCJ010000000
VERSION     DASUCJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117496"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFU90_02520"
     CDS             complement(<1..473)
                     /locus_tag="VFU90_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478673.1"
                     /translation="MAVRAPQVSPGREFSQLLDIFPLAIRQALVRLANVEDIIEVVLD
                     LGRPPEARFEHDFTYLTDTPVTHEDIAHVCSRRSSFGADNRAGIEQTLHRISAIRNRT
                     GKIVGLTCRVGRAVYGTIDILLDVLRSGKSICLLGRPGVGKTTMLRECARILSEDR"
     gene            complement(483..2015)
                     /locus_tag="VFU90_02525"
     CDS             complement(483..2015)
                     /locus_tag="VFU90_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5478674.1"
                     /translation="MDQSRTPYFQALLDYVDSGVLPFHTPGHMQGNGMDLSFREFIGD
                     NFCAIDLTPMPGIDDLLQPLESIREAQELAAEAWGADHTFFLINGSTSGNQCMMMTAV
                     NPGDEIAVPRNSHKSMLGGLVMSGARPIYMQPEVDEELHMDHCVTPETIARTLEEHPG
                     IVAVYVVSPTYYGVAADLAGIERVVHGAGKLLLVDEAWGPHFHFHPGLPLSATAAGAD
                     LCINSTHKMLSAMSQCAMLHQKGSRIRLDRLKSVLKMFLSTSPNLPMVASLDVARKQM
                     ATEGTQLLSRTIELAQRTRERINRIDGLYCLGDDMRGRKGVFDLDPTKIAVTVKGLGY
                     TGYEASEILRHRYNIQVELADLFNVVALFTIGTTTEAADRLATAFEELARDERPVDLY
                     APSGILERRLARGSYRLPKIPPMRMLPRDAFLADTEFVRFKDSKGRICAETISPYPPG
                     IPVIAPGEEITTELVDYLRLEMKAGVRIQGAYDDELKTVRVVKEGLTREPDNAPTSTP
                     WK"
     gene            complement(2068..3588)
                     /locus_tag="VFU90_02530"
     CDS             complement(2068..3588)
                     /locus_tag="VFU90_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478675.1"
                     /translation="MKRSRPWAVPAALLAAGFAIRAALYFPLAAFPIDSDGVLAGLCA
                     FQIGDGHYPAFFPGGSRLSAASCYVAAAYFRLLGPGRAGLALVGLTWALLYLIFSYAF
                     LRAALGRRAANVAFLYAAVPAAAFVTVTYVPWAYGEIAASCAATLWLAALWRAQGRAW
                     QRVAFGISAGFGIWISLQTLMIALPAFAWIALSRRAPTVREAPLALLGAVAGALPFWL
                     GNVGRGFPSFTQNWAALPAADPGMVWKNFVWLIGAPLPQLLFSGTSGWAAAVIAAGCV
                     AAAAGFVLALRDRDRDTGATVGARGAGVLLALVMVFSIGLYAASQAGSIRGWTVRYIA
                     PLYVAMPLLLGIGTCAWWRRQRWAAVATAAALIVPNVLQYSLPGSPARAVLTAQLQTD
                     AALRALLARRHVTLVYGDYFLVYHLNFDSRRSVAGIPTVAVVDYLDYAATLPPRGVRW
                     ALLGQDRATLLAWARAADARGSLTPVDGLELFVAGEPAAGSGALLTTLRRASLQVP"
BASE COUNT          679 a         1209 c         1252 g          578 t
ORIGIN      
        1 cgatcctccg acagaatacg cgcgcattcg cgcagcatcg tcgtctttcc gacgcccggg
       61 cggccgagca gacagatcga ttttccgctg cgcagcacgt cgagcaagat gtcgatcgtc
      121 ccgtagaccg cgcgtcctac ccggcaggtg aggccgacga tcttgccggt gcggttgcgt
      181 atcgcgctga tccggtgaag cgtctgctcg atccccgcgc ggttgtcggc gccgaacgac
      241 gatctgcgcg aacagacgtg tgcgatgtct tcgtgggtta ccggcgtgtc ggtgagatac
      301 gtgaaatcgt gttcgaagcg agcctcgggc ggccgtccga ggtcgagaac gacttcaatg
      361 atgtcttcga cgttggcgag ccggacgagc gcctgccgaa tggcgagggg gaagatatcg
      421 aggagttggg agaattcccg gccgggggaa acctgtgggg ctcgaaccgc caatactgac
      481 cctcacttcc atggggtact cgtgggtgcg ttatccggtt cgcgcgtaag tccttcctta
      541 accacgcgca cggtctttag ctcgtcgtcg tacgcacctt gtatgcgtac gccggccttc
      601 atttccaagc gcaggtagtc gacgagttcg gtggtgattt cctcgccggg cgcgattacc
      661 ggaatgccgg gcggatacgg cgagatggtt tcggcgcaaa tccgtccctt cgaatctttg
      721 aaccgcacga actcggtatc ggcaaggaac gcatcgcgcg gcagcatgcg catcggcgga
      781 attttcggca ggcggtagct cccgcgggcg agacggcgct cgagaattcc ggagggtgcg
      841 tacagatcga ccggacgctc gtcgcgcgcc agctcttcga aggcggtggc cagccggtcc
      901 gccgcttcgg tcgtagtgcc gatcgtaaag agtgcgacga cgttgaacag gtcggcgagc
      961 tcgacttgga tgttgtagcg gtggcgcaga atctcggagg cctcgtaacc cgtgtacccc
     1021 aagcctttga cggttaccgc gatcttcgtc ggatcgagat cgaacacgcc cttgcgcccg
     1081 cgcatgtcgt cgcccaggca gtacagaccg tcgatccgat tgattcgctc ccgcgtccgt
     1141 tgcgccagct cgatggtccg cgagagcagc tgggtgccct cggtcgccat ctgcttgcgc
     1201 gcgacgtcca gcgacgccac catcggcaaa tttggcgacg tcgaaagaaa catcttgagc
     1261 accgacttga gccggtcgag ccggatccgg cttccctttt ggtgcagcat cgcacactgc
     1321 gacatcgccg agagcatctt gtgcgtcgag ttgatgcaga ggtcggcgcc ggcggcggtg
     1381 gcagacagcg gcaggccggg gtggaagtgg aagtgcgggc cccaggcttc gtcgacgagc
     1441 agcaacttgc cggctccgtg aacgacccgt tcgattcccg caagatcggc ggcgacgccg
     1501 tagtaggtgg gcgagacgac gtacaccgcc acgatcccgg gatgctcttc gagcgtgcgc
     1561 gcgatcgtct cgggggtcac gcagtgatcc atgtgcagct cttcgtcgac ttcgggctgc
     1621 atgtagatcg gccgcgcacc gctcatgacc agtccgccga gcatcgattt gtgcgagttc
     1681 cggggaaccg caatctcgtc gccgggattt acggccgtca tcatcatgca ctgattgccg
     1741 ctggtcgagc cgttgatcag aaaaaaggtg tggtcggcgc cccaggcctc ggcggccagc
     1801 tcttgggctt cccggatcga ttccaacggc tggagcagat cgtcgatccc gggcatcggc
     1861 gtaaggtcga tcgcacagaa attgtcgccg atgaactcgc gaaacgaaag gtccatcccg
     1921 ttgccctgca tgtggccggg cgtatgaaac ggcagcacgc ccgaatcgac gtagtcgagc
     1981 agtgcctgaa agtagggcgt gcgagattga tccatgtcgt gaaagtcctc tcgcgagtac
     2041 gccggtgcgc gctcgaagcc ccgccgccta aggaacctgg agcgacgcgc gccgcagcgt
     2101 cgtcagcagc gcgccgctgc cggcagccgg ttcgccggcc acgaacagct ccaagccgtc
     2161 gaccggcgtc agcgaaccgc gcgcgtccgc ggcgcgcgcc caagcgagca gcgtcgcccg
     2221 atcttggccg agcaatgccc agcgcacgcc gcgcggcggc agggtcgcgg cgtaatcgag
     2281 gtagtcgacc accgccaccg tcgggatgcc ggcgacgctt cgccggctgt cgaagttgag
     2341 atggtacacc aggaaataat cgccgtacac gagcgtgacg tgccggcgcg cgagcagcgc
     2401 gcgcagcgca gcgtcggtct gcagctgggc ggtcagcacg gcgcgcgccg ggctgccggg
     2461 caagctatac tgcagcacgt tggggacgat cagcgcggcg gccgtggcga cggcggccca
     2521 gcgttgccgg cgccaccagg cgcacgttcc gatccccagc agcagcggca tggcgacgta
     2581 gagcggcgcg atgtagcgca ccgtccagcc gcgaatcgag ccggcctgcg acgcggcgta
     2641 gaggccgatc gagaacacca tcacgagcgc gagcaaaacg ccggcgccgc gcgctccgac
     2701 cgttgcgccg gtatcgcgat cgcggtcgcg cagagccagt acgaaaccgg cggcggccgc
     2761 aacgcagccc gccgcgatga cggcggcggc ccagccggag gttcccgaga acagcagctg
     2821 cggcagcggc gcgccgatca gccagacgaa atttttccac accatcccgg gatccgcagc
     2881 cggcagcgcg gcccagtttt gcgtgaacga aggaaaaccg cggccgacgt ttccgagcca
     2941 aaacggcagc gcgccggcga ccgcgccgag cagcgcgagc ggcgcctcgc gcacggtcgg
     3001 cgcccggcgc gacagcgcga tccacgcgaa cgcgggcagc gcgatcatca gcgtctgcag
     3061 cgaaatccaa atgccgaatc cggccgaaat gccgaacgcc acgcgctgcc aggcgcgacc
     3121 ctgcgcgcgc caaagcgccg cgagccacag cgttgcggcg cacgatgcgg cgatctctcc
     3181 gtacgcccac ggcacgtagg tcaccgtcac gaaagcggcc gccggaaccg ccgcgtagag
     3241 aaaggcgacg ttggcggccc tgcgtccgag cgccgcacgc aaaaacgcat agctgaagat
     3301 cagatagagc agcgcccagg tcagcccgac cagcgcgagt cccgcgcggc ccggcccgag
     3361 cagccggaag tatgccgcgg cgacgtagca gctggcggcg ctcagtcgcg agcctcccgg
     3421 gaagaacgcg gggtagtgcc cgtcgccgat ttgaaacgcg cacagtccgg ccagcacgcc
     3481 gtcggaatcg atcggaaagg ccgccagcgg gaaatacaac gccgcgcgga tcgcaaatcc
     3541 cgccgccagc agcgcggcgg ggacggccca cggccggcta cgcttcaacc cgttcgtcga
     3601 tcgaaacgtc gtacaggaag aggaacttac cccactcgtc gggcagcgtg ttgcgcttga
     3661 tctgaatcca gggaatgtat ttgggctgac gcggtttgcg cttgagcgac atgttcgc
//
LOCUS       DASUCJ010000202         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1117511, whole genome shotgun sequence.
ACCESSION   DASUCJ010000202 DASUCJ010000000
VERSION     DASUCJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117511"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFU90_02535"
     CDS             complement(<1..415)
                     /locus_tag="VFU90_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478676.1"
                     /translation="MHVNRREFLRKSICAALGGASLYSAIGSLKLIGAAAAAQRGFSD
                     YKALVCVFMYGGNDSFNTIVPMSGPDLTNYLASRGDLAITTGQHALNPASGGGPANYG
                     LHPSMPELATLFNSGKAAVVANVGSLLYPTSQSDYQ"
     gene            complement(426..>1233)
                     /locus_tag="VFU90_02540"
     CDS             complement(426..>1233)
                     /locus_tag="VFU90_02540"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEU5478677.1"
                     /translation="MTHALDNIFNHQNVGPFIAMRLIERLVTSNPTPQYVQRVATVFN
                     NNGSGVRGDLKAVVKAILLDPEARYGNVMFPTTFGKLREPLLKITQMWRAMEARSIGG
                     RIGTLAPWPPIEEQIGQAPQRSPTVFNFFKPDFEQPGEVEQRGLVSPEFQILTDTAVV
                     ATPNYFFHEVFCDYTNPPSDGCYASDDPSTMQMNEDRDAALAATDPAGLVDQYNALFM
                     SGQMSPFMRDVLITRLNLLTEDNYGQDLGRTRVQHALYLILNSPEYSIQK"
BASE COUNT          215 a          372 c          419 g          227 t
ORIGIN      
        1 tctggtagtc ggactgcgac gttggataga gcaggctgcc gacgttcgcg acgaccgcgg
       61 ccttgccgct gttgaacagg gtcgcaagct cgggcatcga gggatgcagg ccatagttcg
      121 ccggaccgcc accggacgcc ggattcagcg cgtgctggcc ggtcgtgatg gcaagatcgc
      181 cgcgcgaggc gaggtagttc gtgagatcag gcccgctcat cggcacgatc gtgttgaagc
      241 tgtcgttgcc gccgtacatg aagacgcaga ccagcgcctt gtaatcgctg aagccgcgtt
      301 gcgccgcggc cgcggcgccg atcagcttca gcgagccgat cgcggaatag aggctcgcgc
      361 cgccaagcgc ggcacagatg ctcttgcgca ggaattcgcg acgattcaca tgcatggccg
      421 cggcctcact tctggatcga gtattcgggg gaattgagga tcaggtacag cgcatgctgc
      481 acccgcgtac ggccgagatc ctggccgtag ttgtcctcgg tcaaaagatt caggcgcgtg
      541 atcagcacgt cgcgcatgaa cggcgacatc tggccggaca tgaagagcgc gttgtactga
      601 tcgacaagac ccgcgggatc ggtcgctgca agcgcggcgt cgcgatcctc gttcatctgc
      661 atcgtggacg gatcgtcgct cgcgtagcag ccgtccgacg gcgggttcgt gtagtcgcag
      721 aacacctcgt ggaagaagta gttcggcgtc gcgacgaccg cggtatcggt caggatctgg
      781 aactcggggc tgacgagccc gcgctgctct acttcgccgg gctgctcgaa atcgggcttg
      841 aagaagttga acacggtcgg cgagcgttgc ggcgcctggc cgatctgttc ctcgatcggc
      901 ggccacggcg cgagcgtgcc gatgcgaccg ccgatcgaac gcgcttccat cgcgcgccac
      961 atctgcgtga tcttgagcag cggctcgcgc agcttgccga acgtcgtcgg gaacatcacg
     1021 ttgccgtagc gcgcttcggg atcgagcagg atcgccttga cgacggcctt gagatcgccg
     1081 cgcacgcccg agccgttgtt gttgaacacg gtcgcgacgc gctgcacgta ctgcggcgtc
     1141 ggattgctcg taacgagccg ctcgatcagc cgcatcgcga tgaacgggcc gacgttctgg
     1201 tgattgaaga tgttgtcgag cgcgtgcgtc atc
//
LOCUS       DASUCJ010000203         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1469153, whole genome shotgun sequence.
ACCESSION   DASUCJ010000203 DASUCJ010000000
VERSION     DASUCJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469153"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..744
                     /gene="tsaE"
                     /locus_tag="VFU90_02545"
     CDS             322..744
                     /gene="tsaE"
                     /locus_tag="VFU90_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5478678.1"
                     /translation="MVRRTFSNEREFCDFAAAFARRLRPGDVVALEGPLGSGKTTFVR
                     AVVEALHGVDQTSSPTFTFWHRYQGTPPIDHIDLFRIRNPSERSELGLEDAVGAAGSI
                     ALIEWWRNAPDLVPERRYEIAIEGAGDAPRTLSLTEPA"
     gene            741..1388
                     /gene="tsaB"
                     /locus_tag="VFU90_02550"
     CDS             741..1388
                     /gene="tsaB"
                     /locus_tag="VFU90_02550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5478679.1"
                     /translation="MNVLAIDGALGGFSAAVSRDGSVRAARREDGTVALEAGLSLVRD
                     VMARSGVSAADLDRVAVGTGPGGFTGLRVAIAYAKSLAQAWERPLVAVSSFDLLEYGT
                     DFDPVLAVVVGRPGVVSLRYRTGGAVSRASGRVEEALSAVVPREATSLTAIGASKDVL
                     AALAERGIVVQSLAPAVVPPAAAAALIGATRAPAAAVHAVRADYGELPAATLPKR"
     gene            1401..>1867
                     /gene="rimI"
                     /locus_tag="VFU90_02555"
     CDS             1401..>1867
                     /gene="rimI"
                     /locus_tag="VFU90_02555"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5478680.1"
                     /translation="MKVELNALQARRPERLSIASMTSADVPAVAGIERASFSTVWPSD
                     AFYNELSTNKLAHYFVGRIDDRIVAYGGIWVILEDSHVTTLAVDPEHRGRGFGEALLL
                     RLIDEALERGAAWMTLEVRESNAVAQRLYRKYGFTTVTMRRGYYSDDNESALV"
BASE COUNT          297 a          633 c          617 g          320 t
ORIGIN      
        1 accttgcgtc accgtgtcgg caccgggata gccgttgaac atgttgtcga gcgtgcgatt
       61 ctcctgaatc aaaatgacga cgtgctggat tttctgcccg ggtacggcgg tcggcgtcgg
      121 gccggggccg gggggcaccg atccgcccga cgaactacag cctgcaacgg ccagcgccga
      181 cgcaagggaa accgcaaatc gaacgatgga cgtacgccac atgatattct cccgtcgaac
      241 gttcgcagcg caaggtgccg atttcctcgg tgcgcaagca agcctgagcg ccgcatgaac
      301 gctgaataac cacggtcaac aatggtgcgc cggacgtttt ccaacgaacg agagttttgc
      361 gatttcgcgg cggcgttcgc gcgccgtctc cgtcccggcg acgtggtcgc gctggagggg
      421 ccgctcggaa gcggcaagac gaccttcgta cgcgcggtcg tcgaagcgct ccacggcgtc
      481 gatcaaacgt cgagtccgac ctttacgttt tggcaccgtt accaagggac gccgccgatc
      541 gatcacatcg acttgtttcg cattcggaat ccgagcgagc gcagcgaact cgggctcgaa
      601 gatgcggtcg gcgctgccgg ttcgatcgcc ctgatcgagt ggtggcgcaa cgcgcccgac
      661 ctcgttcccg agcgacgcta cgaaatcgcg atcgaaggcg cgggcgacgc gccgcgcacc
      721 ctttcgctca ccgagcccgc gtgaacgttc tggcgatcga cggagccctc ggcggcttct
      781 cggcggcggt gtcgcgcgac ggctccgtcc gcgcggcgcg ccgcgaggac ggcacggtcg
      841 ctctcgaagc ggggctatcg ctcgtgcggg acgtgatggc acgctcgggc gtttcggccg
      901 ccgatctcga tcgggtcgcg gtcggcaccg gtccgggcgg tttcaccggc ctgcgcgtgg
      961 caatcgcgta cgcgaaatcg ctcgcgcagg cgtgggaacg cccgctggtt gcggtatcgt
     1021 cgttcgatct gctcgagtac ggcacggatt tcgaccccgt cctcgcagtc gtcgtcggcc
     1081 gccccggcgt cgtgtcgctg cgctaccgga cgggcggggc cgtgagccgc gcttccggac
     1141 gggttgagga agcgctctcc gcggtcgtgc cccgcgaggc gacctcgctg accgcaatcg
     1201 gcgcctcgaa ggacgtgctt gcggcgctcg ccgaacgcgg tatcgtcgtg cagtcactcg
     1261 ctcccgccgt cgtaccgccc gccgccgccg cggcgctgat cggcgcaacg cgcgcgccag
     1321 cggccgccgt tcacgcggtc cgggccgatt acggcgaact tcccgcagcc acgctgccaa
     1381 aacgctgatc gaattttccg atgaaagtgg aactgaacgc cctccaagcc cgccgccccg
     1441 aacgtctctc gatcgcgtcg atgacgtcgg ccgacgtacc ggcggtggcc ggcatcgagc
     1501 gcgcttcgtt ttcgaccgtc tggccgtcgg atgcgttcta taacgagctt tcaacgaaca
     1561 agctcgcgca ctatttcgtc ggccgcatcg acgaccggat cgtcgcctac ggcggaatct
     1621 gggtgattct ggaggattcg cacgttacca cgctggccgt cgatccggag caccgcggcc
     1681 gcggcttcgg cgaagcgctg ctgctgcggc tcatcgacga agcgctcgaa cgcggcgccg
     1741 cgtggatgac cctcgaggtc cgcgagagca acgccgtcgc ccagcgtctc tatcgcaaat
     1801 acggctttac gacggtcacg atgcgtcgcg gctactacag cgacgacaac gagagcgcgc
     1861 tcgtcat
//
LOCUS       DASUCJ010000204         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_949488, whole genome shotgun sequence.
ACCESSION   DASUCJ010000204 DASUCJ010000000
VERSION     DASUCJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949488"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1528)
                     /locus_tag="VFU90_02560"
     CDS             complement(77..1528)
                     /locus_tag="VFU90_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5478681.1"
                     /translation="MRFPACGFALVAGFAYAFASAAAAADWPVFGHDPARSGVDAGDT
                     VLTINNVHRLHERWQIAFGSGAVADSTPILLDRVPYRSTHRRMLFQTAKNGTTYGIDA
                     LSGRILWRFATHGPKITTSTPAADPSGTAIYVPGVDGFVHKLDAATGHELRAPGFPAR
                     ITRMVQTEKNASPLNVANGYLYATTSGYLGDAPPYDGHVVGVRLSDGKRRVFNSLCSN
                     YRKLPTPTSCSYSDSGIWSRGGAVVDPDPAMNGSVYASTGNGNFDANQGGYNYGDSVL
                     SLTGDQLDLQSYYTPPDYQKLDQQDLDLGSTSPALLPRQSQSRTPLMLVQGGKDQILR
                     LLNRAPLPGVGGELQEVTLQWPLFSTPAIWIDPSNRVWLFVGFPQAVDAYRLKTQGGV
                     SRLTGIWRSTAGQTFGEGTSPVVSNGIVFVAFDGAIVALDALHGNELWNSSMTGAGKT
                     IGPVHWESPIVADGWVYCSDENGKLTAYALPNR"
     gene            complement(1566..2048)
                     /gene="sixA"
                     /locus_tag="VFU90_02565"
     CDS             complement(1566..2048)
                     /gene="sixA"
                     /locus_tag="VFU90_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEU5478682.1"
                     /translation="MRCYFLRHGLAADAQQWKGDDGDRPLTNEGKRRMEREAKTLAAL
                     DLEIEAIVTSPLLRAKQTAAIVAATLGLRDRAIEDERLGFGFDANALAAILRERAPTA
                     AAVLLVGHEPSMSATIGALAGAERIVLKKGGLACVDISDESCSEGELVWLVPPKILTL
                     "
BASE COUNT          375 a          723 c          714 g          409 t
ORIGIN      
        1 gcagcggctc tcggtccagc cggtgatgcc gcacgaatgg aagatcgttc tcgaacttgc
       61 caaggagtga cgccgctcag cggttgggca acgcgtaggc cgtgagtttg ccgttttcgt
      121 cggagcagta cacccagccg tcggccacga tcgggctttc ccaatgcacc ggcccgatcg
      181 tcttgccggc gccggtcatc gaactattcc acaactcgtt tccgtggagc gcgtcgagcg
      241 cgacgatcgc accgtcgaaa gcgacgaaca cgatcccgtt gctcaccacc ggcgacgtgc
      301 cctcgccgaa cgtctggccg gcggtcgacc gccaaatccc ggtcagccgg cttacgccgc
      361 cctgcgtttt caagcggtag gcgtcgacgg cttgaggaaa cccgacgaag agccataccc
      421 gatttgacgg atcgatccag atagccggtg tcgaaaacag cggccactgc agcgtaacct
      481 cctgaagttc gccgccgacg cccggcagcg gagcgcggtt gagcaagcgg agaatttgat
      541 cttttccgcc ttgcacgagc atcagcggcg tccggctttg gctttgccgc gggagcagcg
      601 ccggcgacgt gctgcccaag tcgaggtcct gctggtcgag tttctggtag tccgggggcg
      661 tgtagtaact ctgcagatcg agctggtcgc cggtcaagga cagcaccgaa tcgccgtagt
      721 tgtaaccgcc ctgattggcg tcgaagtttc cgttgccggt cgacgcgtac acgctcccgt
      781 tcatcgccgg atccggatcg acgaccgctc cgccgcgcga ccaaattccg gaatcgctgt
      841 acgagcacga ggtcggcgtc ggcagcttgc gatagttgct gcacagcgag ttgaacacgc
      901 gccgcttacc gtcgctcaaa cgaacgccca cgacgtgacc gtcgtacggc ggcgcgtcgc
      961 ccaagtatcc cgacgtcgtc gcgtacaagt agccgttggc gacgttcagc ggcgatgcat
     1021 ttttttcggt ctgcaccatg cgcgtgatgc gcgcggggaa tccgggagcg cgcagttcgt
     1081 gaccggtcgc cgcatcgagt ttgtggacga atccgtccac gccggggacg taaatcgccg
     1141 ttccgctcgg atccgctgcc ggcgtggagg tagtgatctt cgggccgtgc gttgcgaacc
     1201 gccacaggat tcgcccggag agtgcatcga ttccgtacgt cgtgccgttc ttggcggtct
     1261 ggaaaagcat tcgccgatgg gtagaccggt acggcacgcg gtcgagcagg atcggggtcg
     1321 agtcggcgac ggcgccgctg ccgaacgcga tctgccagcg ttcgtgcagc cggtggacgt
     1381 tattgatggt caacacggtg tcgccggcgt ccacgccgct gcgcgccgga tcgtggccga
     1441 agaccggcca gtcggcggct gcggccgccg aggcaaacgc gtacgcgaat ccggcgacga
     1501 gtgcgaaacc gcacgctggg aatcgcatga cggacctcct tgccgccgaa aacgcggccg
     1561 gcgtcttaca gcgtcagaat tttcggcggg acgagccaga cgagctcgcc ctccgagcag
     1621 ctttcatccg aaatgtcgac gcaggcgagt ccgccttttt tcagcacgat ccgttcggct
     1681 ccggcgagcg cgccgatcgt cgcgctcatg ctcggctcgt gtcccaccag caagacggca
     1741 gcggcggtcg gcgcgcgctc gcgtaagatc gcagccagcg cgttcgcgtc gaacccgaat
     1801 ccgagccgct cgtcttcgat cgcgcgatcg cgcaggccga gcgttgcggc cacgatcgcc
     1861 gcggtttgct tggcccgcag caacgggctc gtaacgatcg cttcgatctc gagatcgagt
     1921 gccgcgagcg ttttagcctc gcgctccatt cggcgcttgc cttcgttcgt gagcggacga
     1981 tcgccgtcgt cccccttcca ctgctgcgcg tcggcggcta agccgtgacg caggaagtaa
     2041 caccgcacgc tagcctaggg cttgcgcttg cgtaagaaag acgcgctgcg cgtcgaccgg
     2101 catctcaccc ggccccggaa cgcggcgacg ataggtaccg ctttcgagca gttcgcggct
     2161 cttcacgttg tcggcaaata gaacccttag gatttgcccg tcgatcgttt cggcgatcat
     2221 c
//
LOCUS       DASUCJ010000205         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_567625, whole genome shotgun sequence.
ACCESSION   DASUCJ010000205 DASUCJ010000000
VERSION     DASUCJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567625"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>1089)
                     /locus_tag="VFU90_02570"
     CDS             complement(178..>1089)
                     /locus_tag="VFU90_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5478683.1"
                     /translation="RELWRLASSPAEARQIVLTTPLDYKPGTQYVYSDLGAMTLGFLV
                     EKLSGQPLDEYLSMWVFNPLGMTNTFYRPADSVKYRTAPTEDTPPRGYPLQGEVHDEN
                     AYALGGVAGHAGLFSTAADLSIFAQMILNGGEFNGTRIVSDSTIKLFTHRAAGSRALG
                     WAMADGQWGSGRFLSDDAFGHVGYTGTSLWIDPDRDMFVILLTNRVHEPRARRPEKVI
                     ADIRADLSDAASVAVTDDPDQLIAMPVSFRADKASGWNHIERRHHRRVRHHGTKSTVR
                     IAKRQAATPHGKKTVVTTKKSHTTHKK"
BASE COUNT          177 a          354 c          357 g          201 t
ORIGIN      
        1 tcacgcgcct gagcgccgcc ttgaccacgt cctcagtcaa cgcaggcgcg gatcccggcg
       61 cctgaggcgc agggacggaa ggaccagcat tcaattcggg ttggtgtgcg tcgtcgttca
      121 tgcgggctcc ggcagacatc atctgcaact tcaccggcgc cccggaggcg ggcaaggcta
      181 cttcttgtgc gtcgtgtggc tcttcttggt cgtgacgacg gttttcttgc cgtgcggcgt
      241 cgccgcctgc cgctttgcga tgcgcaccgt cgacttggtt ccgtgatgac gcacgcgacg
      301 atgatgacga cgctcaatgt ggttccagcc actcgctttg tcggcgcgga acgacaccgg
      361 catcgcgatc aactgatcgg gatcatctgt caccgctacg gacgcggcgt cggaaaggtc
      421 ggcgcgaatg tcggcgatca ctttctcggg gcgccgtgcg cgcggctcgt gcacgcgatt
      481 cgtgagcaga atgacgaaca tgtcgcggtc gggatcgatc cacagcgacg tgcccgtgta
      541 gccgacgtga ccgaacgcgt cgtcactcag gaagcggccc gagccccact ggccgtcggc
      601 catcgcccat ccgagcgcgc ggctgccggc ggcgcggtgc gtgaacagct tgatcgtcga
      661 gtcgctgacg atgcgcgtgc cgttgaattc gccgccgttc agaatcatct gcgcgaagat
      721 ggacaggtcg gcggcggtgc tgaacagtcc cgcgtgtccg gccacgccgc ctaacgcata
      781 cgcgttctcg tcgtggacct cgccctggag cggatagccg cgcggcgggg tgtcctccgt
      841 gggcgcggtg cggtacttca ccgaatcggc cggccgataa aacgtgttcg tcatgcctaa
      901 cggattgaac acccacatcg acaggtattc gtccaacggc tggccgctta acttctcgac
      961 caggaacccc agcgtcatcg cccccaagtc ggagtagacg tactgcgttc cgggcttgta
     1021 gtcgagcggc gtggtgagca cgatctgacg cgcctcggcc ggcgacgacg ccaagcgcca
     1081 cagctcgcg
//
LOCUS       DASUCJ010000206         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_322679, whole genome shotgun sequence.
ACCESSION   DASUCJ010000206 DASUCJ010000000
VERSION     DASUCJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322679"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFU90_02575"
     CDS             <1..560
                     /locus_tag="VFU90_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5478684.1"
                     /translation="DDCVAAYEWALENAAEIGGDAARIAIGGDSAGGNLAAVVALVAR
                     EQQLPQPKLQLLVYPVTDYEFESAAMIDNAKGYFLEADGMRWFYEHYARTPDDYDDWR
                     FSPLRASDVGGLARAVVVTAEYDPLRDQGEAYGEKLRAAGVRTTIVRAQGLIHGCFGL
                     HPFMPPAQEAWDVAVSALRETLEAG"
     gene            560..>1068
                     /locus_tag="VFU90_02580"
     CDS             560..>1068
                     /locus_tag="VFU90_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEU5478685.1"
                     /translation="MPLHPQAQTIVDALNAADPIPMTPETQRQGFAMLLMLAGEPPAV
                     ASVEDADADGVPVRIYRPFDEVGLPVVVYLHGGGWTIGTVEQFDPVLRQLAVASGAIV
                     VAPEYRVAPEHPFPAPLDDCWQAVQWVAKHAADFGGDGSRLALAGDSAGGNMLAVCAL
                     LARDAGSPD"
BASE COUNT          159 a          345 c          377 g          187 t
ORIGIN      
        1 tcgacgactg tgtcgccgcg tacgaatggg cgctcgagaa cgcggccgaa atcggcggtg
       61 atgctgcgcg aattgcgatc ggcggcgaca gtgcgggagg caatctcgcg gccgtcgtcg
      121 cgctcgtggc gcgcgagcag cagctcccgc aaccgaagtt gcaactgctc gtctatcccg
      181 tcacggatta cgagttcgag agtgcggcga tgatcgacaa cgccaagggc tacttcctcg
      241 aggccgacgg catgcgctgg ttctacgagc actacgcgcg cacccccgat gactacgacg
      301 attggcggtt ctcgccgttg cgggcctccg acgtcggcgg cctcgcgcgc gcggtcgttg
      361 tcaccgccga gtacgacccg ttgcgcgatc agggcgaggc ctacggcgag aagctgcgag
      421 ccgcgggcgt gcgaacgacg atcgtgcgcg cgcagggact catccacgga tgtttcggct
      481 tgcatccctt catgccgccc gcgcaggaag cgtgggacgt cgccgtatcc gccttgcgcg
      541 agacgctcga ggccggctga tgccgttgca cccgcaagcg caaacgatcg tcgacgcgtt
      601 gaacgcggcg gacccgatcc cgatgacgcc ggagacgcag cgtcaaggct tcgcgatgtt
      661 gttgatgctc gccggcgagc cgcccgcggt tgcatcggtc gaagatgccg acgccgacgg
      721 tgtcccggta cgcatctacc gtccgttcga tgaagtaggt cttccggttg tcgtctacct
      781 ccacggcgga ggttggacga tcggcacggt cgagcagttc gatccggtgc tgcgtcagct
      841 tgcggtcgcg tcgggtgcga tcgtcgtcgc gcccgagtat cgcgttgcgc cggagcatcc
      901 gtttccggcg ccgctcgacg attgctggca ggcggtgcaa tgggttgcga aacacgccgc
      961 cgacttcggt ggcgacgggt ctcggctcgc gttggccggc gacagcgcgg gcggcaacat
     1021 gttggccgtc tgcgcgttgc tggcgcgcga cgccgggtcg cccgacat
//
LOCUS       DASUCJ010000207         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1469212, whole genome shotgun sequence.
ACCESSION   DASUCJ010000207 DASUCJ010000000
VERSION     DASUCJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469212"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFU90_02585"
     CDS             complement(<1..572)
                     /locus_tag="VFU90_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU5478686.1"
                     /translation="MEESRPVRAIYRYAFLTRASHWVWVLAFAVLVSSGLQIFNASPR
                     LDASDKSDAARRVLAIESPEDGVGTTTLFGRTFVTTGWLGWTDDGMGSRGAHAFPAWL
                     TIPGYQDLAGGRRWHLFFAWVATVCWLAWLLSAALKGSLREMVLRPSDLPKLWPMQAY
                     YLKLRKTPPPHGTYNPLQKAAYTLVMFAIAP"
     gene            632..1414
                     /locus_tag="VFU90_02590"
     CDS             632..1414
                     /locus_tag="VFU90_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5478687.1"
                     /translation="MAEPTVSLTFAGGVARVTLARPDVRNAFNAAVIAELHAAFTRIS
                     TADDVRAVVIAGEGTVFCGGADVNWMRESLDLPFEANAIDAEKMSDMFRAIDNCPRPV
                     IARIQGAALGGGAGLAAVCDIAIAADDAVFGFTEVKLGIIPAVISPFVLSKIGSSQAR
                     ALFLTGERFDAARAASIGLVHRVVAAGELDAAVDRVLAEIRTAGPYAVSAAKLLVRRV
                     LDATYQESRAITTQAIANQRISAEGQEGLRAFLERRKASFHE"
     gene            1411..3363
                     /locus_tag="VFU90_02595"
     CDS             1411..3363
                     /locus_tag="VFU90_02595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5478688.1"
                     /translation="MIRRLLIANRGEIAVRVARAAREMGIVPVGIYSEADADAYHLRF
                     VDDARCVGPAPAPESYLDVEAVVAAAVAMNADAVHPGYGFLSERAVFARAVAAAGITF
                     VGPSPEAMDAMGSKIEAKRRVREFDVPTVPGYDGDDQSLATLRAQAERVGFPLLVKAS
                     AGGGGRGMRVVESLAQFDDALAAAKREALAAFGDDAVLLERYLREPRHIEFQVLGDAF
                     GTLVHLGERECSIQRRHQKIVEEAPSVALSPQLRAEMGAAAVRAAASVGYVNAGTCEF
                     MLDGDGSYYFLEMNTRLQVEHPVTEFVYGVDLVQWQLRIASGERLTLAQQDVRPRGWA
                     IEGRVYAEDPANHLLPSTGTISRWAPPEGPGVRVDAGVTTGSAVGVYYDPMLAKLIVY
                     GSDRAQAIARFEQALREFTVSGVRTNVPLLLWIARDEAFRAGATTTAFLAQRLDESIF
                     AAAAAPREALLLCAAGLLLDGRAPWRIGGVGVPLRLTCGDAPAGELLADATAQPGVWR
                     LSGNVAGELHAQRRGDTVSAGFGDRGQTKTFTGTVVPEGGDVLVRYDGGTYVFRLAAP
                     PSVGSADAAAGGALDGRVTAPMPGKIVKIAVREGDAVAERALLVVLEAMKMEHRIEAS
                     TGAKVKAVLVKEGEIVAGGAPLVELA"
     gene            3373..4305
                     /locus_tag="VFU90_02600"
     CDS             3373..4305
                     /locus_tag="VFU90_02600"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5478689.1"
                     /translation="MQPKAVTLFEMGPRDGLQNEDEIVPAPDKVRFIDLLSETGLRWI
                     EATSFVSPKAIPQLADASDVFARIRKAPGVRYPVLTPNIKGYERAKAAGADAIAVFTA
                     ASERFTKRNINMTIDESLGVFGDVVRAAKADGMWVRGYVSTAFGSPFGDEVTPRDVVG
                     VSVKLMDLGCDALSIGDTIGVGVPSQVDELVPLLAGKIPLDRIAFHFHDTRGTALANV
                     YAALRNGIAAFDSSTGGLGGCPYAPGATGNVGTEDVLYMLERMGIETGVDLAKVRAAS
                     RFIATVVGHAPTSEAFTAMEAADARSAAPGGAGP"
BASE COUNT          721 a         1479 c         1476 g          676 t
ORIGIN      
        1 ggcgcaatcg cgaacattac cagcgtgtag gcggctttct gcaacgggtt gtaggtgccg
       61 tgcggcgggg gcgttttgcg cagcttcaga tagtacgcct gcatcggcca cagtttcggc
      121 aggtcgctcg gccgcagcac catctcgcgc aagctgccct tgagggccgc cgatagcagc
      181 cacgccagcc agcacaccgt cgccacccac gcgaagaata agtgccagcg ccggccgccg
      241 gccaggtctt gataaccggg aatcgtgagc caagcgggaa acgcgtgtgc gccgcgcgag
      301 cccattccgt cgtcggtcca tccgagccag ccggtggtga cgaacgtgcg gccgaaaagg
      361 gtggtcgtgc cgaccccatc ctcgggcgat tcgatcgcca gcacgcgacg cgcggcatcg
      421 ctcttgtcgg aggcgtccaa gcgcggcgac gcgttgaaaa tttgcagccc gctcgaaacg
      481 agcacggcga aggccagcac ccagacccaa tggctggcgc gggtaaggaa ggcgtaacgg
      541 tagatcgcgc gcaccggccg gctttcttcc acgacgctag cataacgctt tccaggcgcg
      601 attggatcga atcgagaatc ggcgcagggc catggccgaa ccaaccgtct ccttgacctt
      661 cgccggcggg gtcgcgcgcg tcacgctggc gcgccccgac gtgcgcaatg ctttcaacgc
      721 cgccgtgatc gcggagctgc acgccgcgtt cacccgcatc tccaccgccg acgacgtgcg
      781 ggcggtcgtg atcgcgggtg aggggacggt cttttgcggc ggcgccgacg tcaactggat
      841 gcgcgaatcg ctcgacctgc cgttcgaagc caacgcgatc gacgccgaaa aaatgagcga
      901 catgttccgc gcgatcgaca actgcccgcg gccggtcatc gcccggattc aaggtgcggc
      961 gctcggcggc ggcgcagggc tggccgcggt ttgcgatatc gccatcgccg ccgacgacgc
     1021 cgtcttcggc ttcaccgagg taaagctggg catcatcccg gcggtgattt cgccgttcgt
     1081 gctctcgaag atcggctcgt cgcaggcacg tgcgctgttc ctcaccgggg agcgcttcga
     1141 cgccgcccgg gccgcgagca tcggcttggt gcaccgcgtc gtagccgccg gcgagctcga
     1201 cgccgcggtc gatcgcgtgc tggcggaaat ccgcaccgcc ggaccgtacg cggtgagcgc
     1261 cgccaaactg ctggtgcgcc gcgtactcga cgcaacctac caagaatcgc gagcgatcac
     1321 gacgcaggcg atcgccaatc agcgcatcag tgcggaaggc caagaagggc tgcgcgcgtt
     1381 tctcgagcgg cgtaaagcca gcttccacga atgatccgac ggctgctgat cgccaaccgc
     1441 ggcgaaatcg cggtgcgcgt cgcgcgggcc gcccgcgaga tgggtatcgt gccggtcggc
     1501 atctactccg aggccgacgc cgatgcatac caccttcggt tcgtcgacga cgcgcgctgc
     1561 gttgggcccg cgcccgcgcc ggagtcgtac ctcgacgtcg aggcagtcgt cgccgccgcg
     1621 gtggcgatga acgccgacgc ggtgcatccc ggttacggct ttctttccga gcgggcggta
     1681 ttcgcgcgcg ccgtcgccgc ggcagggatc acgttcgtcg gcccgagccc cgaagcgatg
     1741 gacgcgatgg gcagtaaaat cgaagccaag cgccgagtgc gcgagttcga cgtccccacg
     1801 gtccccggct acgacggcga cgatcaatcg ctcgcgacgc tgcgcgcgca ggccgagcgc
     1861 gtcgggtttc cgctgctcgt caaagcgagt gccggcgggg gcgggcgcgg gatgcgcgtg
     1921 gtcgagtcgc tcgcgcagtt cgacgacgcg ctcgccgccg ccaaacgcga ggcactggcc
     1981 gcgttcggcg acgacgccgt gctgctggaa cgctacctgc gcgagccccg ccacatcgag
     2041 ttccaagtgc tgggcgacgc gttcggtacg ctcgttcacc tcggcgagcg cgagtgctcg
     2101 attcagcgtc gccaccagaa aatcgtcgaa gaggcaccct cggtcgcgct ctcaccgcaa
     2161 ctacgcgccg agatgggcgc cgcggcggtg cgggccgccg cctcggtggg ctacgtcaac
     2221 gccgggacct gcgaattcat gctcgacggc gacggcagct actattttct cgagatgaac
     2281 acgcggctgc aagtagaaca cccggtcacg gagttcgtct acggcgtcga cctcgtgcag
     2341 tggcagctgc ggatcgcgag cggcgaacgg ctcacgctcg cgcaacagga cgtacggccg
     2401 cgcggctggg cgatcgaagg ccgcgtctac gccgaagatc ccgccaacca tttgctcccg
     2461 tcgaccggca cgatttcgcg ctgggcgccg ccggagggcc cgggcgtacg cgtggacgcc
     2521 ggcgtgacga ccggcagcgc agtgggcgtg tactacgatc cgatgctcgc gaagctgatc
     2581 gtttacggca gcgaccgcgc ccaagcgatc gcgcgcttcg aacaagcgct gcgcgaattt
     2641 accgtgagcg gcgtgcgcac caacgtgccg ctcctgctgt ggatcgcccg cgacgaagcg
     2701 ttccgcgcgg gcgcgacgac caccgcgttt ctcgcgcagc ggctggacga gtcaattttc
     2761 gcggccgccg ccgcgccacg cgaagcgctg ctgctgtgcg cggccggctt gttgctcgac
     2821 ggacgtgcgc cgtggcgcat cggcggcgtc ggcgttccgt tgcgactgac gtgcggcgac
     2881 gctccggccg gcgagctcct cgccgacgct accgcgcagc ccggggtgtg gcggctgagc
     2941 ggcaacgttg ccggcgaact gcacgcccaa cggcgcggcg acaccgtgtc ggccggcttc
     3001 ggcgatcgcg gccaaacgaa aacattcacg ggtacggtcg tacctgaagg cggcgacgtg
     3061 ctggtacgct acgacggcgg cacgtacgtc ttccggttgg ccgctccgcc gtcggtcgga
     3121 agcgccgacg cggcggccgg cggtgcgctc gacggccgcg tcaccgcgcc gatgccgggg
     3181 aaaattgtaa agatcgcggt acgtgagggc gacgcggtcg ccgaacgcgc gctgctcgtc
     3241 gtactcgaag cgatgaagat ggaacaccgg atcgaagcct cgaccggggc caaggtgaaa
     3301 gcggtgctcg tgaaggaagg ggaaatcgtc gccggcggcg cgccgctggt cgagctcgca
     3361 taaacttcaa aaatgcagcc aaaagccgtt acgctcttcg agatgggccc tcgcgacggg
     3421 ctgcagaacg aagacgaaat cgttcccgcc cccgataaag ttcgctttat cgatctgctt
     3481 tccgaaacgg gattgcgctg gatcgaggcg acgtcgttcg tcagccccaa ggcgattccg
     3541 cagctggccg atgcgagcga cgtgttcgcg cgcatccgca aagcgcccgg cgtgcgctat
     3601 ccggtgctga cgcccaacat caaaggttac gaacgcgcga aggcggcggg cgccgacgcg
     3661 atcgcggtct tcaccgccgc atccgaacgc tttaccaagc gaaacatcaa catgacgatc
     3721 gacgaatcgc tcggcgtctt cggcgacgtc gtgcgcgccg ccaaagccga cgggatgtgg
     3781 gtacgcggct acgtctcgac cgcgttcgga tcgccgttcg gcgacgaagt tacgccgcgc
     3841 gacgtggtcg gcgtcagcgt caagctgatg gatctcggct gcgacgcgct ctcgatcggc
     3901 gacacgatcg gcgtcggcgt gccgagccaa gtggacgagc tcgtgccgtt gctggccggc
     3961 aagattccgc tcgaccggat cgcgtttcac ttccacgata cccgcgggac cgcgctcgcc
     4021 aacgtctacg ccgcgctgcg caacggcatc gccgccttcg attcgtccac cggcggcctc
     4081 ggcgggtgcc cgtacgcgcc cggggcaacc ggcaacgtcg gcaccgaaga cgtcctttac
     4141 atgctcgagc ggatgggtat cgaaaccggc gtcgatctcg ccaaggttcg tgccgcctca
     4201 cgcttcatcg caaccgtcgt ggggcacgcg cccaccagcg aagcgtttac cgcgatggaa
     4261 gccgccgacg cgcgctcggc tgcacccggc ggcgccggtc cttagcggta gtccacctcg
     4321 gtcatcatgc cgtccatcat gtgcacttcg tt
//
LOCUS       DASUCJ010000208         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_873288, whole genome shotgun sequence.
ACCESSION   DASUCJ010000208 DASUCJ010000000
VERSION     DASUCJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873288"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1663)
                     /locus_tag="VFU90_02605"
     CDS             complement(<1..>1663)
                     /locus_tag="VFU90_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5478690.1"
                     /translation="AAGVTSTSLGLQPIRGPVPHATPAPGESRVFRRPDGAYQAIPDV
                     AGKTKTFHIVERAAPWTLKPGLTVMANTYNGVVPGPTLVVDQGDSVVIDYRNEDQTPD
                     TVHLHGIHDIPDSMDGVAGISQPLVPRGGRFTYRFVAEMPGTFIYHSHDKEAMLNSGL
                     YGAVVVEPARPLPVERGLSHDFLEIVSSWQIQSAAENEFTLNGKEYPATQTLDVRRGE
                     RFRIRWINISGENFHTMHTHGHYQQIVARDAAPVAAPELEDTVLLGPGQRVDVVVKAD
                     AQPGTWLLHCHVLDHIENAQGMPDGLITAIHYDGTPNTLGKMYPAMMPAANATAVKPL
                     SFAMTVLLGAIAGFTIFLGLPIARAQRVSPQTIALLNALAIGILLYLVIEIAQNAILP
                     VVRGINAWHGGAAPFPGWLVTVFVAGLLLGLVGLGSVATHMTRSAAAHAENPMLLSTI
                     VAIGIGAHNFGEGLAIGASAASGATAIALALIVGFALHNATEGFGVAAPLVGRFMPSW
                     SQIALAGLIAGGPTFVGTIVGYAFYSPVLSVLFLATAVGALIFVIG"
BASE COUNT          279 a          545 c          570 g          269 t
ORIGIN      
        1 cgccgatcac gaagatcagc gccccgaccg cggtcgccaa gaacagcacc gacagcaccg
       61 gcgagtagaa cgcgtagccg acgatcgttc ccacgaaagt cggcccgccg gcaatgagcc
      121 ccgcgagcgc gatttgcgac caggacggca tgaagcgtcc gaccagcggc gcggcgacgc
      181 caaacccttc ggtcgcgttg tgcagcgcga aaccgacgat cagcgcgagc gcgatggcgg
      241 tcgcacccga ggcggctgag gcgccgatcg cgagcccctc gccgaagttg tgcgcgccga
      301 tgccgatcgc gacgatcgtc gagagcagca tcgggttttc ggcgtgagcg gcggcgctgc
      361 gcgtcatgtg ggtcgcgacg ctgccgagcc cgacgagtcc gagcagcagc ccggcgacga
      421 aaaccgtgac gagccatccc gggaagggcg ccgcgccgcc gtgccacgca ttgatccctc
      481 gaaccaccgg cagaatcgcg ttttgcgcga tctcaatcac cagatagagc agaattccga
      541 tggccagcgc gttgagcagc gcgatcgttt gcggcgaaac gcgctgcgcg cgggcgatcg
      601 ggagcccgag gaagatcgta aagccggcga ttgcgcccag cagcaccgtc atcgcgaagc
      661 tcaagggttt gaccgcggtg gcgttggccg ccggcatcat cgcggggtac atctttccga
      721 gcgtgttcgg ggtgccgtcg taatgaatcg cggtgatgag cccgtcgggc attccctgcg
      781 cgttttcgat gtgatccagc acgtgacagt ggagcagcca cgtacccggc tgcgcgtcgg
      841 ctttcacgac gacgtcgacg cgctgtcccg gccccagcag caccgtgtcc tcgagttcgg
      901 gcgcggccac cggcgcggcg tcgcgtgcca cgatctgctg gtagtgcccg tgggtgtgca
      961 tcgtgtgaaa attttcgccc gagatgttga tccagcgaat gcgaaagcgc tcgccgcggc
     1021 gcacgtcgag cgtttgggtg gcgggatatt ccttgccgtt gagcgtgaac tcgttttcgg
     1081 cggcgctttg aatctgccac gacgagacga tctcgaggaa gtcgtggctg aggccgcgct
     1141 cgaccggcag cggacgcgcc ggctcgacca cgaccgcgcc gtacagtccc gagttcagca
     1201 tcgcttcctt atcgtgcgag tgatagatga acgtccccgg catctcggcg acgaagcgat
     1261 acgtaaaccg gccgccgcgc ggcaccagcg gctgcgaaat gccggccacc ccgtccatcg
     1321 agtcgggaat gtcgtgaatc ccgtggagat gcacggtatc gggcgtttgg tcttcgttgc
     1381 gatagtcgat gaccaccgag tcgccctggt ctaccaccag cgtcggcccc ggtacgaccc
     1441 cgttgtacgt gttggccatc accgtcaggc ccggcttgag cgtccacggg gcggcccgct
     1501 cgacgatgtg aaacgttttg gtcttgcccg cgacgtccgg gatcgcttga taggcgccgt
     1561 cgggccgccg gaagacgcgc gattcgcccg gcgcgggggt ggcgtgcggc accggccccc
     1621 ggatcggctg cagcccgagg ctggtggacg tgaccccggc cgc
//
LOCUS       DASUCJ010000209         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_873290, whole genome shotgun sequence.
ACCESSION   DASUCJ010000209 DASUCJ010000000
VERSION     DASUCJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873290"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1158
                     /locus_tag="VFU90_02610"
     CDS             127..1158
                     /locus_tag="VFU90_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5478691.1"
                     /translation="MASWALRLLSSGAAGLALAAATPDAGGTLSSVAAEYYAKVGAIA
                     LVTGRDTTMDYYQRLLDDEDLLKLPVPDGYTPSAWQYSVQNIARLDLSLANQLLHQTS
                     QPMAQVRGLGETLVRSSKDGTMQPVAVYVPSTYSPGRPAPLIVFLHGHPQSESELIAP
                     EFIADLAERTGTILVAPYGRGYYDYRGSESDVYDALAAAKTAFSTDPRKRYLAGYSMG
                     GFSVFSVAPVHPNEWAGIMAIAGALLGSLSHNVTAMLPNTPFYVLTGARDESIPTQYP
                     TATAVFLRNAGVPVTFYSDPNATHRLSTLRTILAQAWSDMENGIVRTPYQLRATVPLL
                     GVQPPTMKT"
     gene            1162..1614
                     /locus_tag="VFU90_02615"
     CDS             1162..1614
                     /locus_tag="VFU90_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEU5478692.1"
                     /translation="MNWYLLMKFLHVLLAIAALGANLTYGVWFARANADPAFAAAALR
                     GIKFIDDRIANPAYVLMLPTGAAMVMLGGLSFRTPWIAWAMGLWAVAILAAYLGYTPA
                     LRAQIQAVEREGIASGAARRLAVAGNVWAAVLGLLVAAILVLMIFKPA"
     gene            complement(1601..>2045)
                     /locus_tag="VFU90_02620"
     CDS             complement(1601..>2045)
                     /locus_tag="VFU90_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478693.1"
                     /translation="GGVLVGALSQRLGRRACVLICALLGAALVPLWAHSQTVALLALG
                     AFCMQLAVQGAWGVVPAHLNELSPAGARGTFPGFTYQLGNAISAGAAQIEAAFALRFH
                     LPGGGADYGKAMGIIALTTFAFVFALAALGYLVAREGRHDELTPA"
BASE COUNT          340 a          643 c          709 g          353 t
ORIGIN      
        1 acaggctgtc gaaactgcga ctctcttgga tgatgacgac gacgttggcg attcttgagg
       61 ccggcccgcg gcgccgggcg cgggtctccc gtcacgccgg cgacctccaa gcgtgggaat
      121 aaggttatgg cgtcgtgggc attgcgtctc ctgtcgtccg gcgccgcggg gctggcgctg
      181 gcggccgcta ctccggatgc cggcggaacg ctgagctcgg tagcggcgga gtactacgcg
      241 aaggtggggg ccatcgccct ggtaaccggc cgcgacacga cgatggacta ctaccagcgg
      301 ctgctggacg acgaggactt gctcaagctc cccgtgcccg acggttacac gccgtcggcg
      361 tggcagtact ccgtgcaaaa catcgcgcgg ctcgatctca gtctggcgaa ccagctgctg
      421 catcaaacct ctcagccgat ggcgcaggtt cgcggactgg gagaaacgct ggtgcgctcc
      481 tccaaggacg ggacgatgca gccggtcgcc gtgtacgtgc cgagtacgta cagcccgggg
      541 cgtcccgcgc cgttgatcgt gttcttacac ggccatcctc aatccgagag cgagttgatt
      601 gcgccggagt tcatcgccga cttggccgag cggaccggga cgatcctggt ggcgccttac
      661 ggccgcggct attacgatta tcgcggatcg gaatccgacg tgtacgacgc gctggctgcc
      721 gcgaagacgg ccttttcgac ggatccgcgc aagcgttatc tggccggata ttcgatgggg
      781 ggattctcgg tgttttcggt ggcgcccgtg catcccaacg aatgggccgg gatcatggcg
      841 atcgcgggtg cgctcttggg atcgctgtcg cacaacgtca ccgcgatgct gccgaacacg
      901 ccgttctacg ttttgacggg agcccgggac gaaagcattc ctacccagta tccgacggcg
      961 accgcggtgt ttttgcgtaa cgcgggcgta ccggtgacgt tctattccga tccgaacgcg
     1021 acccatcgcc tctccacgtt gcgaaccatt ctggcgcaag cctggagcga catggagaac
     1081 gggatcgtcc gcaccccgta ccagctgcgg gcaacggttc cgctgctagg cgtacagccg
     1141 ccgacgatga agacgtgacc ggtgaactgg tacttgctga tgaaattcct gcacgtgctg
     1201 ctcgcgatcg cggcgcttgg ggcaaatctc acctacggcg tctggtttgc gcgcgccaac
     1261 gcggatccgg cctttgcggc cgcggcactg cgcggcatca agttcatcga cgaccggatc
     1321 gctaatccgg cgtacgtgct gatgctgccg accggggccg cgatggtgat gctcggcgga
     1381 ctttcgttcc ggacgccctg gatcgcttgg gcgatgggat tgtgggcggt cgccattttg
     1441 gcggcgtatc tgggctacac gccggcgctg cgcgcgcaaa ttcaggcggt ggagcgcgag
     1501 ggaattgcga gcggcgcggc gcgaaggctg gccgtcgccg ggaacgtctg ggcggcggtg
     1561 ctcggtttgc tcgtcgcggc gatcttggtg ctgatgatat tcaagccggc gtaagctcgt
     1621 cgtgccggcc ctcgcgcgcc acgagatagc cgagcgccgc gagagcgaag acgaaagcaa
     1681 acgtcgtcaa cgcgatgatg cccatcgcct tgccgtagtc ggcgccgcct ccaggcaggt
     1741 gaaagcggag cgcaaaggca gcctcgatct gcgccgcacc ggcgcttata gcgttgccga
     1801 gctgataggt gaaaccgggg aacgtgccgc gcgcgccggc cggcgagagc tcgttgaggt
     1861 gcgcggggac gacgccccac gctccctgca cggccagctg catgcagaac gcgccaagcg
     1921 cgagcagcgc gacggtttga ctgtgagccc agagcggaac gagcgcggcc ccgagcagcg
     1981 cgcagatcag cacgcaggcg cgccgcccga ggcgttggga aagcgcgccg acgagcaccc
     2041 ctccg
//
LOCUS       DASUCJ010000210         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1224821, whole genome shotgun sequence.
ACCESSION   DASUCJ010000210 DASUCJ010000000
VERSION     DASUCJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224821"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFU90_02625"
     CDS             <1..266
                     /locus_tag="VFU90_02625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEU5478694.1"
                     /translation="LQPRSPEEAERAVAMGFGDLERVLQIDDLVKSDNVIFCATGITD
                     GDLVRGVRFYGNQARTHSIHVHSSGTVRFIESIHRLGARPAGR"
     gene            289..1725
                     /locus_tag="VFU90_02630"
     CDS             289..1725
                     /locus_tag="VFU90_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5478695.1"
                     /translation="MKRYAHQMVVVGAGSAGYASARTAREFGCDVALVDHGPLGGLCI
                     LRGCMPSKALLASSDALADAREARALGIHAERIGIDMPFIARRKRALVKEFADYRIDG
                     IEEFPLYTGSARFLSPTELAVGDDVVLEAPQFVIATGSAVATERCPGLAETGYVDSDA
                     VLELERIPASAIVLGGGFTACELGQFLSRMGARTTMLIRSGHLLTSGDDDVGDALTEY
                     FREENIEIVARTALMEARRSGPEKIVRYLAGGEPGEVAAEEIFYALGRTPNVAGLDLE
                     KAGIAYDTVRGIAVDETLRTSNPNVFAAGDVTGDYMLVHVAIYQAEVATRNACLQARE
                     SADYRLVSAHTVFTDPQVAVAGLTEKDLRESGIPYVSGRYDFAEHGKAQCLGKTKGFV
                     KMMAEPEAGKILGAAAIGPQASELIHEVIVAMNFDATVAQFMRIPHLHPTLAEIWTYP
                     AELCAAQLGMKVPGDEQLEVATSVEARR"
BASE COUNT          338 a          596 c          644 g          286 t
ORIGIN      
        1 gcctgcagcc gcgcagcccg gaggaggcgg agcgcgccgt tgcgatggga ttcggcgatc
       61 tcgagcgcgt gctgcaaatc gacgaccttg tgaaaagcga caacgtgatc ttctgcgcga
      121 ccgggattac cgacggcgac ctggtgcggg gcgtgcgctt ctacggcaat caggcgcgaa
      181 cgcactcgat tcacgtccac tccagcggta cggtgcgctt catcgaatcg atccaccggc
      241 tcggcgcgcg gccggccgga cgttgacgcg gcaagaccgg acgcgaacgt gaagcgctac
      301 gcgcaccaaa tggtcgtagt cggggcgggt tcggcgggat acgcgtcggc ccgcaccgcg
      361 cgcgagttcg gctgcgacgt ggcgctggtc gaccacggtc cgctcggcgg gctgtgcatc
      421 ttgcgcggct gcatgccgag caaggcgctg ctggcgtcga gcgacgcgct cgccgacgcc
      481 cgcgaggcgc gtgcgctcgg aatacacgcc gagcggatcg gcatcgatat gccgttcatc
      541 gcccggcgca aacgcgcgct ggtgaaggag ttcgccgatt accggatcga cggcatcgaa
      601 gagtttccgc tctataccgg aagcgcgcgc ttcctctcgc cgaccgagct cgcggtcggc
      661 gacgacgtcg tgctggaagc gccgcagttc gtgattgcca ccggaagcgc cgtcgctacg
      721 gaacgctgcc cgggcttagc ggaaacgggc tacgtcgata gcgacgcggt gctcgagctc
      781 gagcgcatcc cggcttcggc aatcgttctg ggcggcggct tcacggcgtg cgagctggga
      841 cagtttctct cccgcatggg ggcgcgcacg acgatgctga tccgaagcgg gcacctgctg
      901 acctcgggcg acgacgacgt cggcgacgcg ctcaccgagt atttccgcga ggaaaacatc
      961 gagatcgtcg cgcgaacggc gctgatggaa gcgcgccgca gcggacccga gaagatcgtg
     1021 cgatacctgg ccggcggcga accgggcgag gtcgccgccg aagaaatctt ctacgccctc
     1081 gggcgcacgc ccaacgtcgc cgggctggat ctggagaagg ccggaatcgc ctacgatacg
     1141 gtgcgcggga tcgcagtcga cgagacgttg cgaacgagca accccaacgt gttcgcggcg
     1201 ggcgacgtga ccggcgacta catgctggtg cacgtagcga tctatcaggc ggaggtcgcg
     1261 acccgtaacg cctgcctgca ggcgcgcgag agcgccgact accggctggt ttccgcccac
     1321 accgtgttca cggacccgca agtcgccgtc gcggggctaa ccgagaagga cttacgcgaa
     1381 agcgggattc cgtacgtctc gggccgctac gatttcgccg aacacggcaa agcccaatgt
     1441 ctcggaaaga ccaagggctt cgtcaagatg atggcggagc ccgaggccgg caagattctc
     1501 ggcgccgccg cgatcgggcc gcaagcttcg gagttgatcc acgaagtcat cgtcgcgatg
     1561 aatttcgatg caaccgtcgc gcagttcatg cgaatcccgc acctgcatcc cacgctcgcg
     1621 gaaatttgga cgtacccggc cgaactgtgc gcggcgcagc tggggatgaa ggttcccggc
     1681 gacgagcagc tggaagtggc gaccagtgtc gaagcacgga gataagtccg aacggccgtc
     1741 tcccgcgcgg cttcgcgtcg cgatcgttca gatgaagcca gccaaggggc gttacgcgga
     1801 aaaccttgat gctgcaaaac gggcgttcgc gcagctggcc gaggctccgc ccgacctgat
     1861 cgtg
//
LOCUS       DASUCJ010000211         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_720316, whole genome shotgun sequence.
ACCESSION   DASUCJ010000211 DASUCJ010000000
VERSION     DASUCJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720316"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..522)
                     /locus_tag="VFU90_02635"
     CDS             complement(55..522)
                     /locus_tag="VFU90_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5478696.1"
                     /translation="MTWTREELQRAHDNYVAVAQEAGRTGNWRPWADLFTQDATYIEH
                     HYGTMRGREAIYEWIAKTMAEWPNSEMKEFPHDWCVCDEERGWWICQIENRFTDPGDG
                     AVYQAYNLTVLQYAGDMQFSSEEDAYNPANFAPVVKAWIAANRRADAAKPETA"
     gene            complement(519..>1126)
                     /locus_tag="VFU90_02640"
     CDS             complement(519..>1126)
                     /locus_tag="VFU90_02640"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5478697.1"
                     /translation="AFGDAPELVVSGINNGCNTGRAILHSGTVGAALTAQSFGASALA
                     VSVDVSDPWRYDTAARVALDVLPMLEQAPPRSVLNLNVPACDYDAVRGVRWARLAAFG
                     SVRAAIAEGERGLQFELRATGVSPPPDSDQGLVEQGYAALTTIVGVAEAWPPEFGAAE
                     GVTSSVVPGVDVEPVHAVPDASAPRSLHRPLHGGAVPKESR"
BASE COUNT          164 a          402 c          367 g          193 t
ORIGIN      
        1 ccggcgtccg gaccctcgtc agcccgccga cccggcgccc atacggctct cttgctacgc
       61 cgtctccggc ttcgcggcgt ccgcgcgtcg attggctgcg atccacgcct tcaccaccgg
      121 cgcgaagttt gcagggttgt acgcgtcctc ctccgaagag aactgcatgt cgcccgcata
      181 ctgcaacacg gtcaggttgt acgcctggta caccgctccg tcgcccgggt cggtgaaacg
      241 gttttcgatc tgacagatcc accagccgcg ttcttcgtca cacacgcacc agtcgtgcgg
      301 aaactccttc atctcggagt tcggccactc ggccatcgtc ttggcgatcc attcgtagat
      361 cgcctcgcgt ccgcgcatcg ttccgtagtg atgttcgatg taggtcgcgt cttgtgtgaa
      421 caggtcggcc cacggtcgcc agttcccggt gcggccggcc tcttgtgcga cggcgacata
      481 gttgtcgtgc gcgcgttgca gttcttcgcg ggtccaggtc atcgcgactc ctttgggacg
      541 gcaccgccgt gcagcggccg gtgcagcgat cgcggcgccg acgcgtccgg cacggcgtgc
      601 accggctcga catccacgcc gggcacgaca ctcgacgtga cgccttcggc ggcgccgaac
      661 tcgggtggcc acgcctcggc gacaccgacg atcgtggtga gcgcggcgta gccctgctcg
      721 acgaggccct gatccgagtc cggcggcggc gacacgcccg tggcgcgcaa ctcgaactgc
      781 aatccgcgtt cgccttcggc gatggctgcg cgcacggagc cgaacgcggc gagacgcgcc
      841 cagcgcactc cgcgcacggc gtcgtagtcg caggcgggaa cgttgagatt caacacgcta
      901 cgcggcggtg cctgctcgag catcggcaac acgtcgagcg cgacgcgtgc cgcggtgtcg
      961 tagcgccacg ggtcgctcac gtcgacgctc accgcgagcg cggacgcacc gaaactctgc
     1021 gcggtgagcg cggcgccgac cgtgcccgaa tgcaggatcg cacggcccgt gttgcagccg
     1081 ttgttgatcc cggacacgac gagctcaggt gcgtcgccga acgcgc
//
LOCUS       DASUCJ010000212         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1056682, whole genome shotgun sequence.
ACCESSION   DASUCJ010000212 DASUCJ010000000
VERSION     DASUCJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056682"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFU90_02645"
     CDS             <1..373
                     /locus_tag="VFU90_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478698.1"
                     /translation="AVAVAIFAIVTILTMRAARRTADAPAVRPAGGWTHAAGDDFAGL
                     TEPARCDMVFAMAALDDDASRDVLRCALDDPSETVALAAAHALASRGHAAVLERYFAA
                     HPGERATRIAQTLALLDGPSA"
     gene            complement(391..>1814)
                     /locus_tag="VFU90_02650"
     CDS             complement(391..>1814)
                     /locus_tag="VFU90_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5478699.1"
                     /translation="ANDLPVVAVARLSELLAELPGVDLEVQPVRDYPHGPEGSHLFGY
                     VGAINEQEYERLKNQGYSPNDVIGKDGLEYEYDRYLRGTPGGQRVVVDSSGAVVSTVS
                     SQAAIPGDTLVTDIDWRLQQIVEEELAHGIASWGHGRALSGAVVVEDPWTGGILALAS
                     YPNFDPQDFAAGNFRRIASYLTSPTQPLFDRAIAAATPTGSTFKMVTGSAALSEGVVK
                     VNQVVYDTGGWNCGGYYARDIAAGGLGNTTFIPALAASSDGYFFRLSWWLGNDRLRKY
                     ALAFGLDARSGIDLPGENEGNWPTNAWSMRTFGVPMEPSDACFLGIGQGAMQATPLQI
                     ANVASTVINGGTLYSPQIAREIRSPDGRIVKTFAPRVVRHVPVTQEALKAVREGMARV
                     TDPGGTAYGLAIPGLKFSGKTGTAETAGGNGPNTTWFVAWAPSDHPKLAMAVFVDRSG
                     GYGAQVAAPIARAILIRYFKPKP"
BASE COUNT          287 a          639 c          588 g          300 t
ORIGIN      
        1 cgccgtcgcc gtcgctatct tcgcgatcgt cacgatcctt acgatgcgcg ccgcgcgccg
       61 gaccgccgac gcccccgcgg tgcggccggc gggcgggtgg acgcacgccg cgggcgacga
      121 tttcgccggt ctcacggaac cggcgcgctg cgacatggtc ttcgcaatgg cggccctcga
      181 cgacgacgcg tcgcgcgacg tcctccggtg cgcgctcgac gatccttcgg aaacggtcgc
      241 gctggcggcc gcgcacgccc tcgccagccg cggacacgcg gcggtgctgg agcggtattt
      301 cgccgctcat ccgggcgagc gcgcgacgcg catcgcccaa acgctcgcgc tgctcgacgg
      361 accgtccgcc tagttgcgcc ggctcgcggt ttagggtttg ggtttgaagt aacggatcaa
      421 aatcgcacgc gcgatcggcg ccgcgacctg cgcaccgtaa ccgccgctgc gatcgacgaa
      481 caccgccatc gcaagtttgg gatggtcgct cggcgcccag gcgacgaacc acgtcgtatt
      541 cggaccgtta ccgccggccg tttcggcggt cccggtcttc cccgaaaact tcaggcccgg
      601 gatcgccagg ccgtaggcgg tgccgcccgg atcggtaacg cgcgccattc cctcgcgcac
      661 cgctttgagc gcctcctgcg tgaccggaac gtgccgaacg acgcgcggtg cgaacgtctt
      721 gacgatccgt ccgtccgggc tgcgaatctc tcgcgcgatc tgcggcgagt agagcgttcc
      781 gccgttgatc accgtcgagg cgacgttggc gatttgcagc ggagtggctt gcatcgcgcc
      841 ctggccgatc cccaagaagc aggcgtcgct cggctccatc ggaacgccga aggtgcgcat
      901 cgaccacgcg ttggtcggcc agttcccctc gttttcaccc ggcaaatcga tgccgctgcg
      961 ggcatccagc ccgaaggcca gcgcgtattt gcgaagccgg tcgtttccaa gccaccacga
     1021 taggcgaaaa aagtagccgt cgctcgatgc ggccagcgcc gggatgaacg tcgtgttccc
     1081 caagcctccg gcggcgatgt cgcgagcgta gtagccgccg cagttccagc cgccggtgtc
     1141 gtagacgacc tgattcacct tcaccacgcc ttcgctgagc gcggccgatc cggtcaccat
     1201 cttgaacgtc gagccggtcg gcgtggccgc tgcgatcgcg cgatcgaaca acggctgcgt
     1261 cggactcgta aggtacgacg cgatgcggcg gaagttgccg gccgcgaaat cttgcggatc
     1321 gaagttcgga taactcgcga gcgccaggat cccgccggtc cacgggtcct cgacgacgac
     1381 cgcacccgag agcgcgcggc cgtgccccca cgacgcgatt ccgtgcgcga gctcctcctc
     1441 gacgatttgc tgcaggcgcc agtcgatatc ggtcaccagc gtgtcgccgg gaatcgccgc
     1501 ttgcgacgac acggtcgaga cgaccgcccc gctcgaatcg acgacgaccc gctgaccgcc
     1561 gggcgtcccg cgcaggtagc ggtcgtactc gtactccagg ccgtccttac cgatcacgtc
     1621 gttgggcgaa tatccctgat ttttgaggcg ttcgtactcc tgttcgttga tcgcgccgac
     1681 gtagccgaac agatgcgagc cttccgggcc gtgcggataa tcgcgtaccg gctgcacttc
     1741 cagatcgacg ccgggcagct cggccaacag ttccgaaaga cgcgcgacgg cgacgaccgg
     1801 gagatcgtta gcca
//
LOCUS       DASUCJ010000213         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_873318, whole genome shotgun sequence.
ACCESSION   DASUCJ010000213 DASUCJ010000000
VERSION     DASUCJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873318"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFU90_02655"
     CDS             complement(<1..1081)
                     /locus_tag="VFU90_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5478700.1"
                     /translation="MPASQTLGHVLTVTGSQMTVGFEHEGAEREIRIGDMVKVETEER
                     TVVGTIAEVRFADEGGEQTRMFVVDLMGEIYQRGGEPEFTRGVSFHPAPGDAVLTADD
                     ADLNVVYNESARANLAVGTLHHDPSRPACILTDELLNMHFAVLGTSGSGKSCSISLIL
                     GAVLDNHPNAHIVMFDPHNEYSRAFGDRAEVLSVDTLNLPLWLFNLEELVNVLVRGGS
                     DREQESQAIILKDAIIWARRYYAGDDYGATSITADTPVPFRVHELVRYINDEMGRLSK
                     PDTSIPYLRLRMRIESLRGDRRYSFLFGAEEDTLTDIIGRLLRMPVNGRPMSIVDLSG
                     VPSEIADVVVSVLTRVIFDFSMWSRR"
     gene            complement(1081..>1329)
                     /locus_tag="VFU90_02660"
     CDS             complement(1081..>1329)
                     /locus_tag="VFU90_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478701.1"
                     /translation="LAARVKSGGAVHVKILSPRNGMRIALTDTQSHEVTGLDVGDDAT
                     TVTLKAPAVTLPTRYTVVASFTDGFGQESIVEPITVTP"
BASE COUNT          227 a          422 c          451 g          229 t
ORIGIN      
        1 cgcgccgcga ccacatgctg aagtcgaaga tcacgcgcgt gagcacggaa accacgacgt
       61 cggcgatctc tgacgggacg cccgagagat ccacgatcga catcggccgg ccgttgaccg
      121 gcatgcgcag caggcgcccg atgatatcgg tgagcgtgtc ttcttcggcg ccgaagagga
      181 acgaatagcg ccggtcgccg cgcagcgact cgatccgcat ccgcaagcgg aggtacggaa
      241 tcgaggtgtc gggcttggac agccggccca tctcgtcgtt gatgtagcgc accagctcgt
      301 gaacgcggaa cggcaccggc gtgtcggcgg tgatcgaggt agcgccgtaa tcgtcgccgg
      361 cgtagtagcg gcgcgcccag atgatggcgt ccttgaggat gatggcttgc gactcttgct
      421 cgcggtcgga cccgccgcgc accagcacgt tgaccagctc ctcgagattg aacagccaca
      481 gcggcaagtt gagcgtgtcg acgctgagca cttcggcgcg gtcgccgaag gcgcgcgaat
      541 attcgttgtg cggatcgaac atcacgatgt gggcgttcgg gtggttgtcg agcaccgcgc
      601 ccaaaatcag gctgatcgag cacgacttac cggagccgga ggtgccgagc accgcgaagt
      661 gcatgttcag cagctcgtcg gtgaggatgc aggccggtcg cgacggatcg tgatggagcg
      721 tgccgaccgc caggttcgcg cgcgcgctct cgttgtaaac gacgttcaaa tcggcgtcgt
      781 cggccgtcag gaccgcatcg cccggggccg gatggaagga gacgccgcgg gtgaactcgg
      841 gctcaccgcc gcgctggtag atctcgccca tcaaatcgac gacgaacatc cgggtctgct
      901 cgccgccttc gtcggcgaag cgaacttcgg cgatcgtgcc gacgacggtg cgttcctcgg
      961 tttcgacctt caccatgtcg ccgatccgga tttcgcgttc ggcgccttcg tgttcgaagc
     1021 cgaccgtcat ctgcgaaccg gtgacggtga gcacgtggcc gagcgtttgc gaggccggca
     1081 ttacggcgtc accgtaatcg gctcgacgat gctctcttgc ccgaagccgt cggtaaagct
     1141 cgccacgacc gtgtagcgcg tcggcaacgt caccgccggc gccttgagcg tcaccgtcgt
     1201 ggcgtcgtcg ccgacgtcga gaccggtcac ctcgtgcgac tgcgtgtcgg tcagcgcgat
     1261 gcgcatgccg ttgcgcggcg agagaatctt cacgtggacg gctccgccgc tcttcacgcg
     1321 cgccgcgag
//
LOCUS       DASUCJ010000214         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1484651, whole genome shotgun sequence.
ACCESSION   DASUCJ010000214 DASUCJ010000000
VERSION     DASUCJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484651"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>2318
                     /gene="uvrA"
                     /locus_tag="VFU90_02665"
     CDS             13..>2318
                     /gene="uvrA"
                     /locus_tag="VFU90_02665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5478702.1"
                     /translation="MITGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLSVMEKP
                     DVDHIEGLSPAISIEQKSTSHNPRSTVGTITEIYDYLRLLFARVGTPRCPDHGIPLAA
                     QTVSQMVDATLAMNPERRFMLLAPVVRERKGEHVQVFEQLRAQGFVRARVDGVLHELD
                     AVPALALRVKHTIEAVIDRFRPAPDLKQRLAESFETALRLGDGIAIVADMDDASESDE
                     IRKPTLFSSRYSCPVCDYSLPELEPRLFSFNSPMGACGTCDGLGVTQVFDAARVVVHP
                     NLSLAAGAVRGWDRRNMYYFQMILSLARHYKFDIDTPWEQLPEGIRHAVLFGSGDEQI
                     AFRYLTESGGNVTRKHRFEGIVPNLERRYRETESPAVREELSKFISERPCPDCHGERL
                     NRSARNVFVGESNLPALTGLPIDQALEFFHALVLPGWRGEIAAKIVKEIRERLRFLVD
                     VGLDYLTLDRQADSLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLGT
                     LTRLRDLGNTVIVVEHDEDAIRAADHIVDIGPGAGVHGGEVVAQGKLKDVLAAKRSQT
                     GDFLSGRRSIPVPRKRRAPDPQLMLRVFGASGNNLKDVDLEIPAGLFVCVTGVSGSGK
                     STLINDTLFRYTAAELNGANEKPAPFRKIEGLEHFDKVVDIDQSPIGRTPRSNPATYT
                     GLFTPLRELYAQVPEARARGYQPGRFSFNVKGGRCEACEGDGLIKVEMHFLPDVYVPC
                     DVCHGKRYNRETLEILYKGHTISDVLEMT"
BASE COUNT          398 a          793 c          733 g          394 t
ORIGIN      
        1 gacagtctga tcgtgatcac cggcctgtcc ggctccggca aatcctcgct ggcgttcgac
       61 acgatctacg ccgaaggcca gcgccgctac gtcgagtcgc tctcggccta cgctcgccag
      121 ttcctgtccg tgatggagaa gcccgacgtc gaccacatcg agggcttgtc gccggcgatt
      181 tccatcgaac agaaatcgac ctcgcacaat ccgcgttcga ccgttggcac cattaccgag
      241 atctacgatt acctgcgcct gctgttcgcg cgcgtgggca cgccgcgctg cccggatcac
      301 ggcattccgc tggccgcgca aacggtcagt cagatggtcg acgcgacact ggcgatgaat
      361 ccggaacgcc gtttcatgct gctcgcgccc gtggtgcgcg agcgcaaggg cgagcacgtg
      421 caagtgttcg agcaattgcg ggcgcagggc ttcgtgcgcg cgcgcgtgga cggcgttttg
      481 cacgagctcg acgcggtgcc ggcgctggcg ctgcgcgtca agcacacgat cgaggccgtg
      541 atcgaccgct tccgtcccgc gccggatctg aaacagcgcc tcgccgaatc gttcgagacc
      601 gcgctgcgcc tcggcgacgg catcgcgatc gtcgccgaca tggacgatgc gagcgagtct
      661 gatgagattc ggaaaccgac gctgttttcg tcgcgctatt cctgcccggt ttgcgattac
      721 tcactgcccg aactcgagcc gcgcctgttc tcgttcaatt caccaatggg tgcctgcggc
      781 acctgcgacg gactgggcgt gacccaggtg ttcgatgcgg cgcgcgtggt cgtgcatccg
      841 aatctcagtc tcgccgccgg cgcggtgcgc ggctgggatc gccgcaacat gtactacttc
      901 cagatgatcc tttcgctggc gcggcattac aagttcgaca tcgatacgcc gtgggaacaa
      961 ttgcccgagg gcatccgcca cgccgtgctg ttcggctccg gagacgagca gatcgccttt
     1021 cgctatctga cggaatcggg cggcaacgtc acccgcaagc accgtttcga gggcatcgtg
     1081 ccgaatctcg agcgccgcta ccgcgagacg gaatcgccgg cggtgcgcga ggaattgtcc
     1141 aagttcatca gcgagcgtcc ctgccccgat tgccacggcg aacgcctgaa ccgcagtgcg
     1201 cgcaacgtct tcgtcggcga gagcaacctg cccgcgctga ccggcctgcc gatcgaccag
     1261 gcactggaat tcttccacgc cctcgtcctg cccggctggc gcggcgagat cgcggccaag
     1321 atcgtcaagg aaatccgcga gcgcctgcgt ttcctcgtcg acgtcggcct cgactacctc
     1381 acgctcgacc ggcaggccga ttcgctgtcc ggcggcgagg cgcagcgcat ccgccttgct
     1441 tcgcagatcg gcgcgggcct ggtcggcgtg atgtacgtgc tcgacgaacc gtccatcggc
     1501 ctgcaccagc gcgacaacga acgtctgctc ggcacgctca cgcgcctgcg cgaccttggc
     1561 aataccgtga tcgtggtcga acacgacgag gacgcgatcc gcgccgccga ccatatcgtc
     1621 gacatcggcc ccggcgccgg cgtgcacggc ggcgaagtcg tcgcgcaggg caagctcaag
     1681 gacgtgctcg cggcgaaacg ttcgcagacc ggcgatttcc tgtccggacg gcgctcgatc
     1741 ccggtcccgc gcaagcgccg cgcaccggac ccgcagctga tgctgcgtgt gttcggcgcg
     1801 tcgggcaaca acctgaaaga cgtggacctg gaaattcccg ccggcctgtt cgtgtgcgtg
     1861 accggcgtgt cgggctcggg caagtccacg ctcatcaacg atacgctgtt ccgctacacc
     1921 gcggccgaac tcaacggagc gaacgagaag cccgcgccgt tccgcaagat cgagggactg
     1981 gagcatttcg acaaggtcgt cgacatcgac cagtcgccga tcgggcggac accgcgcagc
     2041 aatccggcca cctatacggg actgttcacg ccgttgcgcg agctctacgc gcaggtgccc
     2101 gaggcgcgcg cgcgcggcta ccagccgggc cgcttcagct tcaacgtcaa gggcgggcgc
     2161 tgcgaagcct gcgagggcga cggcctgatc aaggtcgaga tgcacttcct gcccgacgtc
     2221 tacgtgccct gcgacgtctg ccacggcaag cgctacaacc gcgagacgct ggaaatcctc
     2281 tacaagggcc acacgatttc cgacgtgctg gaaatgac
//
LOCUS       DASUCJ010000215         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_322766, whole genome shotgun sequence.
ACCESSION   DASUCJ010000215 DASUCJ010000000
VERSION     DASUCJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322766"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFU90_02670"
     CDS             <1..946
                     /locus_tag="VFU90_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478703.1"
                     /translation="GFAARAAGTFVALFQPNVGAGLVSQLGRVRVAAAIFAGAAGAYA
                     AGAAIAGWGWPLEYARILTAHANVERFSAIQLTPAAIAHGAGASPASAAAAGLLCAAG
                     AIVAAAVLWRRVRDPFARFASFSCLAPLVATFFHEHDLVVAYAAAAWCGVRTRGAVRA
                     LALFATLLAGVDWLGLAQRPNGVAQSALLAGALAFAFAGLGNRSPSHRSLAAVTACAA
                     LFFFGAWLGVGHPAPVWPDALGAFHAPPNAAASAVWLAEQRLSGLLAVNPVWAFLRAL
                     SLAGSALLALAIYLHYPRHPEQDVTASFDCAPAALRSG"
     gene            complement(1028..2656)
                     /locus_tag="VFU90_02675"
     CDS             complement(1028..2656)
                     /locus_tag="VFU90_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5478704.1"
                     /translation="MTLRPAVYRAASILAVVGMLCSCTKAGTAPGAPTHSLTIADGTG
                     DVPTLNPHLFTETTIGYISELTQAYLVKYDSDNRPYPELVTVVPTQSNGGISPDGKTI
                     TWHLRKGVRWSDGAPFNADDVVFSTKAVLNPANNEVGRDGWNLITKVDEPDKYTVIYH
                     LRRPYSSYIPTFFASAGANPDVLPAHLLAKYPNINQVPYNAKPVGIGPFRVVIWHRGD
                     NIELEANPYYFRGQPRIKKVTFKLVPSVDTLATLMQTGEVQLWPVVPSSMLDRMLALS
                     NVKTDIQTSPFYAHLDFNVTRPLVSDLRVRQAIQLAIDRPELARKISHGHAVVQQSVV
                     PSVVPFAPKDIPFVAFDPNKAKQLLDDAGWKVGPDGIRVKNGQRLSLQFPYFTGSASA
                     DDAVEFIRQALHTVGIDVQTRKFAPAVFFAPYQQNGIVYGSKWDMTYFSWQALPVADI
                     SNLWECNQIPPNGQNVTHFCNKELDSLLEQVKGTYDEAKQRELVDRELKIIDANVPTI
                     VLDVPQVGYTHSPNLTGFHPGAWTPFDNMMDVDI"
     gene            complement(2691..>3404)
                     /locus_tag="VFU90_02680"
     CDS             complement(2691..>3404)
                     /locus_tag="VFU90_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5478705.1"
                     /translation="VALAYATNRQEIIDKIDHGSAISAETDQQPKLSWAYTSDIQHHP
                     YDLEKAKQILDADGWKVGPDGIREKNGQKLEFTLSTQTESNSGKAVQTYLQREWRDIG
                     VQVDVKNYPSSEFFDNSTNGTLQGGHYDAAIFSWIGAADPDDNAIYSGDNLAPHGQNA
                     MMWNNRTATAAMADALKTIDQARRKRDYVIVQQQLAIDVPTIIISFARVPYVYNTDLK
                     GFTASPVISGFWNTWDYSI"
BASE COUNT          511 a         1059 c         1129 g          705 t
ORIGIN      
        1 cggtttcgcg gcgcgcgcag ccggcacgtt cgtcgcgctg tttcagccca acgtcggcgc
       61 cggactggtt tcgcagctgg gacgcgttcg ggttgcggcc gcgatcttcg ccggtgccgc
      121 gggcgcgtac gctgcgggtg ccgcgatagc cgggtggggc tggccgttag agtatgcccg
      181 gatcctgacc gcacacgcga acgtcgagcg cttcagcgcg atccaactca ccccggcggc
      241 gatcgcacac ggcgccggag cgtcgccggc gagcgctgcc gctgccgggc ttctgtgcgc
      301 agccggcgcg atcgtggcgg ccgccgttct ctggcggcgc gtccgcgatc cgttcgcgcg
      361 gtttgcatcc ttctcgtgcc tcgcgccgtt ggtcgcaacg ttctttcacg agcacgactt
      421 ggtcgtcgcc tatgcggcgg ccgcttggtg cggcgtgcga acccgcggcg ccgtccgcgc
      481 cctcgccctt ttcgcaacgc tgttggccgg tgtagattgg ctcgggctcg cgcaacgtcc
      541 gaacggcgtc gcccagagcg cgttgctcgc gggagcgctc gcgtttgcgt tcgccgggct
      601 cggaaatcgc tcgccctcgc accgatcgct tgccgcggtt acggcgtgcg ccgcgctctt
      661 cttctttggc gcctggctcg gcgtcggcca tccggcgccg gtatggcccg acgcgctggg
      721 tgcgtttcac gccccgccca acgctgcggc atccgccgtg tggttggccg aacagcggct
      781 cagcggactc ctcgccgtca atccggtgtg ggcgttctta cgcgcgctct cgctcgccgg
      841 ttcggcgctg ctcgcgcttg caatctatct gcactatccc cgacatcccg agcaggacgt
      901 cacggcgtcc ttcgactgcg cgcctgcggc gctccgctca ggatgacaca gcggggcgct
      961 ccgttcagga tgacacggcg tccttcgact gcgcgcctgc ggcgctccgc tcaggatgac
     1021 actgagccta gatgtcgacg tccatcatgt tgtcgaacgg cgtccacgcg cccggatgaa
     1081 agccggtaag gttcgggctg tgcgtgtagc cgacttgcgg aacgtcgagc acgatcgtcg
     1141 gcacgttggc gtcgatgatc ttcagctcgc gatccaccag ctcgcgctgt ttggcctcgt
     1201 cgtacgtgcc cttgacttgc tcgagcagcg agtcgagctc tttgttgcag aaatgggtta
     1261 cgttttgacc gttgggcgga atctggttgc actcccaaag gttcgagatg tccgccaccg
     1321 gcagcgcctg ccacgagaaa tacgtcatgt cccacttgga cccgtagacg atgccgttct
     1381 gctgatacgg cgcgaagaac acggccggtg cgaacttgcg ggtttgaacg tcgatgccga
     1441 cggtgtgcag agcctgacgg atgaactcga cggcgtcgtc ggcgctggcc gagccggtaa
     1501 agtaagggaa ctgcagcgac agccgctgac cgtttttcac gcggattccg tccgggccga
     1561 ctttccagcc cgcgtcgtcg agcagctgtt tggccttatt cggatcgaaa gccacgaacg
     1621 gtatgtcttt gggggcgaac ggcacgaccg aaggcaccac cgactgctgc acgacggcgt
     1681 gaccgtgcga tatcttcctg gctagctccg ggcgatcgat cgccagctgg atcgcttgcc
     1741 gtacgcgcag gtcgcttacc agcggccgcg tgacgttgaa atcgaggtgc gcgtaaaacg
     1801 ggctcgtttg gatgtcggtc ttgacgttcg agagcgccag catccgatcc agcatcgacg
     1861 atggaacgac cggccagagc tgcacctcgc cggtctgcat cagcgtcgcc agcgtatcga
     1921 cggacggcac gagcttgaag gtaaccttct tgattctcgg ctgcccacgg aagtagtacg
     1981 gattggcttc cagctcgata ttgtcgccgc gatgccagat aacgacccgg aagggaccga
     2041 tcccgaccgg cttggcgttg tagggcactt ggttgatgtt cggatacttc gccagcaggt
     2101 gagccggcag cacgtccgga ttggcgcccg cgctcgcgaa gaaggtcggg atataggacg
     2161 agtacggccg gcgcaaatgg tagatgacgg tgtatttgtc gggctcgtcg actttcgtaa
     2221 tgaggttcca gccgtcgcgt ccgacttcgt tattggccgg attgagcacg gctttggtcg
     2281 agaagacgac gtcgtcggcg ttgaacggcg cgccgtccga ccagcgtacg cctttgcgaa
     2341 gatgccaggt gatcgttttt ccgtcggggc tgatgccgcc gttggattgg gtcggaacga
     2401 ccgtcaccaa ttccggatac ggccggttgt ccgagtcgta cttcacgagg tatgcctgcg
     2461 tcagttccga gatgtacccg atggtcgtct cggtgaacag gtgcgggttg agcgtcggga
     2521 cgtcgccggt gccgtcggcg atggtcagcg aatgcgtcgg cgctcccggt gcggtaccgg
     2581 ccttcgtgca cgagcagagc atccccacca ccgccaggat cgaggcggcg cggtaaacgg
     2641 cgggccttag cgtcatcgag cggactcctt cggtaagcga gcgacggcgg tcaaatcgaa
     2701 taatcccacg tattccagaa gcccgagatc accggcgatg cggtgaaccc cttcaggtcg
     2761 gtattgtaga cgtacgggac gcgggcgaag ctgatgatga tggtcggcac gtcgatcgcc
     2821 agctgctgct gcacgatcac gtaatcgcgc ttgcggcgcg cctggtcgat cgtcttgagc
     2881 gcgtcggcca tcgccgcggt cgcggtgcga ttgttccaca tcatggcgtt ttgaccgtgc
     2941 ggcgccaagt tgtcgcccga gtagatggcg ttgtcgtccg gatccgccgc gccgatccag
     3001 ctgaagatcg cggcatcgta gtgaccgccc tgcagcgttc cgttagtgga attgtcgaag
     3061 aactcgctcg acggatagtt cttgacgtcg acctgcaccc cgatgtcgcg ccactcgcgc
     3121 tgcaggtacg tttgcacggc ctttccggag ttcgactcgg tttgcgtgct gagcgtgaac
     3181 tcgagcttct gcccgttctt ctcgcggatg ccgtcggggc cgaccttcca gccgtcggcg
     3241 tcgaggattt gtttggcttt ttcgagatcg tagggatggt gctggatgtc gctggtatac
     3301 gcccacgaga gtttcggctg ctggtcggtt tccgccgaaa tcgccgaacc gtggtcgatc
     3361 ttgtcgatga tctcctgacg gttcgtcgcg taggcaagcg cgac
//
LOCUS       DASUCJ010000216         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1270765, whole genome shotgun sequence.
ACCESSION   DASUCJ010000216 DASUCJ010000000
VERSION     DASUCJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270765"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2102
                     /locus_tag="VFU90_02685"
     CDS             <1..>2102
                     /locus_tag="VFU90_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5478706.1"
                     /translation="FQAAGFDRETVMNFHFGIPHRRDPGRDDVQLLYVTGNIETQFYS
                     SFNDTFLGPVYPGGVPYLDSTYYNGAIFQTPNPSKLVTGPFPSSPTDRTFNNTTVNGT
                     PRNPSLVGLNQRDGNNVGFSVEKAQYQRNFDNHSYLRVLGYSEWSDWLINGPNGAQLT
                     FAADPAEYDVLAWIYGANAIYSNQLGSKNLFTASLAYVSQKLATYNAEFASGGPNSRA
                     PTGLGTIISSYVGKNGNCYNFTTGAQWSCFDAASQGGLFNLPQSGCPRPQNFPYICLT
                     PGTAPAGSAAAQADAHWVMTEDGRAAQFDGVRPYFSSLALTDVWTPNDKLLVNLGARM
                     DNFVYKFDDTVSGFPARAFWFNAFNNENCGAPAQDPVSRWNGSSFGPCPAGYTPMTVP
                     GVGLANVVPASTSYWVFSPRLAGTYTLNPDNVLRASAGRYVRAAATSSQEINDRQQDL
                     PDQISGFYKLGYTTPYHQNLPDSSWNYDVSWEHHFHGTNISFKLSPFYRATSNQVQYF
                     AIDPATGIVAGVNVGKQVSDGVELSLQSGDMTKNGLSFLLSYTYTDSRIKYAPVSNGV
                     SVLDSLNAAIEQYNSYTHACAGGNADSAICGGGLYAGNAVPTFRNSGGVHVANPYYGG
                     GLQPLIDKNAWFTTYDVIPTAFSSANGFAVPNVATLVLSYKHNRFTVTPNLTYNSGSF
                     YGSPLVWPGYVPQSCTRN"
BASE COUNT          397 a          716 c          616 g          373 t
ORIGIN      
        1 tttccaggcg gccggtttcg accgcgaaac ggtgatgaac tttcacttcg ggatcccgca
       61 tcgccgggat cccggccgcg atgacgtgca gctgctctac gttaccggca acatcgaaac
      121 gcagttctat agctcgttca acgatacgtt cctcggcccg gtctatccgg gcggcgttcc
      181 gtatctcgac tcgacctatt acaacggagc gatcttccaa acgcccaatc cgtcgaagct
      241 cgtcaccgga ccgttcccga gcagcccgac ggaccgcacc ttcaacaaca cgacggtgaa
      301 cggaacgccg cgcaacccgt cgctggtcgg gctcaaccag cgcgacggca acaacgtcgg
      361 cttctcggtc gaaaaggcgc agtaccagcg caacttcgac aaccactcgt acttacgcgt
      421 gctcggctac agcgagtggt ccgactggct gatcaacgga cccaacggcg cgcagctgac
      481 cttcgccgcc gacccggcgg aatacgacgt gctggcgtgg atctacggcg cgaacgcgat
      541 ctactccaac cagctcgggt cgaagaatct ctttaccgcg agcttggcgt acgtttcgca
      601 gaagctcgcg acctacaacg ccgagtttgc cagcggcggg ccgaactcgc gtgcgccgac
      661 cgggctgggc acgatcatct cgagctacgt cggaaagaac ggcaactgct ataacttcac
      721 gaccggcgcg caatggagct gtttcgacgc cgcgagccag ggcgggttgt tcaacctccc
      781 gcaatcgggc tgtccgcggc cgcaaaactt cccgtacatc tgtctgacgc cgggaaccgc
      841 gccggccggc tccgctgccg cgcaagccga tgcgcactgg gtgatgaccg aagacggccg
      901 ggccgcccag ttcgacggcg tccgtccgta cttcagttcg ctcgccctca ccgacgtgtg
      961 gaccccgaac gacaagctgc tggtcaatct cggcgcgcgc atggacaact tcgtctacaa
     1021 gttcgacgac acggtcagcg ggtttccggc gcgcgcgttc tggttcaacg ccttcaacaa
     1081 cgagaattgc ggcgcgcccg cacaagatcc ggtttcgcgc tggaacggca gttcgttcgg
     1141 tccgtgtccc gccggctaca cgccgatgac cgtccccggg gtggggctcg cgaacgtcgt
     1201 tccggcgagc acgtcgtact gggtgttctc gccgcggctc gcgggtacgt acaccctcaa
     1261 cccggataac gtgctgcgcg catcggccgg gcgctacgtg cgcgccgccg ccacctcgtc
     1321 gcaagagatc aacgatcgcc agcaggacct gcccgatcag atatccggct tctacaaact
     1381 cggctacacc acgccgtacc atcagaatct gccggactcg tcgtggaact acgacgtttc
     1441 gtgggagcac catttccacg gaacgaacat atcgttcaag ctctcgccgt tttatcgcgc
     1501 caccagcaat caggtccagt actttgcgat cgatccggcg accggcatcg tggccggggt
     1561 gaacgtcggc aagcaggttt ccgacggcgt cgagctgtcg ctgcagagcg gcgacatgac
     1621 gaagaacggc ctctcgttcc tgctgtcgta cacgtacacc gacagccgga tcaaatacgc
     1681 cccggtctcc aacggcgtca gcgtgctcga cagcctcaac gcggcgatcg aacagtacaa
     1741 ctcctacacg cacgcgtgcg cgggcgggaa tgccgactcg gcgatctgcg gcggcggcct
     1801 ctacgcgggg aacgcggtac ccaccttccg taatagcggc ggcgtgcacg tcgccaatcc
     1861 gtattacggc ggcggccttc agccgctgat cgacaagaat gcctggttta ccacttacga
     1921 cgtgattccg acggcgttca gctcggccaa cgggttcgcg gtccccaacg tggcgacgct
     1981 cgtgctctcc tacaagcaca accggttcac ggtaacgccg aacctgacgt ataattcggg
     2041 gtcgttctac ggttcgccgc tggtgtggcc gggctacgtc ccgcaatcgt gcacgcggaa
     2101 tc
//
LOCUS       DASUCJ010000217         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1209657, whole genome shotgun sequence.
ACCESSION   DASUCJ010000217 DASUCJ010000000
VERSION     DASUCJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209657"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1372)
                     /locus_tag="VFU90_02690"
     CDS             complement(32..1372)
                     /locus_tag="VFU90_02690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5478707.1"
                     /translation="MQGNAAPAREGLAKQALGLSGADQTEVLVSAGDAALTRFTHETS
                     NQNVAGADVTLSVRAIVRNRTGVAQTNRLDERSLRDVVERAIAMARLAPEDPLQPKLP
                     HGDPAPPPPGAYDPETAAAGAEVRARLCDAIFRQAESAGYWSAGFASTSSSGITVANS
                     SGALASFDGTDAAVNVKMNAGDSTGFGESYSTAVAAIDAAAAGKTAVAKARAGAAPRA
                     VDPGPWTVILEPAAFGELFVYLADHFSAQSFDEGSSFCSDGLDRAYFAESVTVRDDYA
                     HPLAPGMPFDFEGQPTQRLALVENGVVRNIVTDSYYAKKLDRPNTGHALPAPNAYGPH
                     ARNLVIDGGTKTVDELVAQTGRGLLVTRFWYIRPVDRKRAIVTGMTRDGTFLIENGKI
                     VHGVRNLRFNDSIVELLNRCEFSREQARTGSYNYSLVVPTAKIAKFNFTSVTDF"
     gene            complement(1362..2813)
                     /locus_tag="VFU90_02695"
     CDS             complement(1362..2813)
                     /locus_tag="VFU90_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5478708.1"
                     /translation="MEFEAEAARALDTAASRGAQYADVRFEVARSERIEVRNGVVATL
                     ADGNTTGFGVRALFDGAWGFAASADTSDAGIDAAAARATEIARASASIARRKIGRIPE
                     RAYVDAFATPMVRDPADVPLGQRVALLLEAERLLHAQSEVTVGRAWLDLWRADKYFYS
                     SIGSRIEQRIRQTGSGVAAMAVGNGDVQVRTYPGDVGLYQSGGWEIVEAARLVEGAPR
                     IAQEAVELLRAPQCPSGTFDLILGGSQMSLQIHESCGHPAELDRVMGWEANFSGTSFL
                     EIDQLGKLKYGSDLVTIVVDNTLAHGMATCGYDDEGTKSVASDIVRGGVLAGYEMSND
                     TARTIDRASNACVRAQSWDCVPMIRMCNLNLLPGTVPFEHLFDDVKDGVYMESNRSWS
                     IDDRRLNFQFGCEMGWEIKNGKRGRLLKNPTYAGMTPQFWNSCDAIADERSWFAWGTP
                     NCGKGEPMQTGRTTQAAAPARFRNVSIGVAYAG"
     gene            complement(2832..>3011)
                     /locus_tag="VFU90_02700"
     CDS             complement(2832..>3011)
                     /locus_tag="VFU90_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478709.1"
                     /translation="VLLDATRLDRPGWRHVVVRFPPGAEITRLIAIYVLPPKGMQLSS
                     GQIEVRNVRAVIAGQ"
BASE COUNT          494 a          988 c          982 g          547 t
ORIGIN      
        1 ggcttagcgg tacggcaaat ggaacgccgc cctaaaagtc ggtgacggac gtaaagttga
       61 acttcgcaat cttggcggtc ggaacgacga gcgagtaatt gtagctcccc gtgcgcgcct
      121 gttcgcgcga aaactcgcag cggttgagca gctcgacgat gctgtcgttg aagcggaggt
      181 tccggacccc gtgcacgatc ttcccgttct cgatcaggaa cgttccgtcg cgcgtcattc
      241 cggtcacgat cgcgcgtttg cggtcgaccg ggcgaatgta ccaaaagcgc gtgaccaaca
      301 gcccgcgccc ggtttgcgcc accagctcgt cgaccgtttt ggttcccccg tcgatgacga
      361 gattgcgagc gtgcgggccg taggcgttgg gtgccgggag cgcgtggccg gtgttcgggc
      421 ggtcgagctt cttcgcgtaa tagctgtcgg tgacgatgtt gcgcaccacg ccgttttcga
      481 ccagcgcgag ccgttgcgtc ggctgtccct cgaaatcgaa cggcatcccg ggcgccagtg
      541 gatgcgcgta gtcgtcgcga accgttacgc tctccgcgaa gtacgcgcgg tccaggccgt
      601 cgctgcagaa cgacgaccct tcgtcgaacg actgcgccga gaagtgatcc gccagataga
      661 cgaacagttc gccgaaggcc gccggttcta aaatgacggt ccagggcccc ggatccaccg
      721 cgcgcggtgc ggcgccggca cgtgctttcg cgacggcggt ctttcccgcg gcggcggcgt
      781 cgattgcagc taccgcggtc gagtacgact cgccgaagcc ggtcgaatcg ccggcgttca
      841 tcttgacgtt gacggcggcg tcggttccgt cgaacgacgc gagcgcgccc gagctgttgg
      901 cgacggtgat gccgctcgac gaggtcgacg caaacccggc cgaccaatac ccggcgctct
      961 cggcttggcg aaagatcgcg tcgcacagac gcgcgcggac ttcggcgcct gcggcggcgg
     1021 tttcgggatc gtaggcaccc ggcggcggcg gagcggggtc gccgtgcggc agcttcggct
     1081 gaagcggatc ttcgggtgcg agccgagcca tcgcgatcgc gcgttcgacc acgtcgcgca
     1141 gcgaccgctc gtcgagccga tttgtttggg ctacgccggt acggtttcgg acgatcgcgc
     1201 gcaccgaaag cgtcacgtcg gctccggcga cgttctgatt cgacgtttcg tgcgtgaagc
     1261 gcgtgagtgc ggcatcgccg gccgagacca ggacttcggt ctgatcggcg ccggacagtc
     1321 cgagcgcttg tttcgcaagc ccctcgcgcg ccggcgcagc gttaccctgc ataggcaacc
     1381 ccgatgctga cgttgcgaaa gcgggccggc gcggccgctt gcgtcgtgcg cccggtctgc
     1441 atcggttcgc cttttccgca gttcggcgta ccccacgcaa accacgagcg ctcgtcggcg
     1501 atggcgtcgc acgagttcca aaattgcggc gtcatgcccg cataggtagg attcttgaga
     1561 agccgtccgc gcttgccgtt cttgatttcc caacccatct cgcaaccgaa ctggaaattc
     1621 agccggcggt cgtcgatcga ccacgagcgg ttgctttcca tgtagacgcc gtctttgacg
     1681 tcgtcgaaga ggtgctcgaa cggcaccgtg ccggggagca ggttcaagtt gcacatccgg
     1741 atcatcggga cgcaatccca gctctgcgcg cggacgcagg cgttgctggc ccggtcgatc
     1801 gttcgcgcgg tatcgttgct catttcgtag ccggcgagca cgccgccgcg aacgatgtcc
     1861 gaagcgaccg atttcgtgcc ttcgtcgtcg tagccgcagg tggccatccc gtgcgccagc
     1921 gtgttgtcga ccacgatcgt caccagatcg gaaccatatt tcaacttccc taactgatcg
     1981 atctcgagaa aactcgtgcc cgaaaagttg gcttcccatc ccatgacgcg atccagttcg
     2041 gcggggtgtc cgcacgactc gtggatttgc aacgacattt gcgagccgcc gaggattaaa
     2101 tcgaaggtgc cgctcggaca ttgcggagcg cgcagcagct cgaccgcctc ttgcgcgatg
     2161 cgcggcgcgc cttccaccag ccgcgccgcc tcgacgattt cccatccgcc ggattgatac
     2221 aacccgacgt cgccgggata cgtgcgcact tggacgtcgc cgttgccgac ggccatcgcg
     2281 gcgacccccg agccggtttg ccggatgcgc tgttcgatcc gggagccgat cgaactgtag
     2341 aaatatttgt cggcacgcca caggtcgagc cacgcgcgtc ccaccgtgac ctcggattgg
     2401 gcgtggagca accgttcggc ctcaagcagc agcgcgaccc gctgccccag cggaacgtcg
     2461 gcgggatccc gtaccatcgg cgtcgcgaag gcgtcgacgt acgcccgctc ggggatccgg
     2521 ccgattttcc ggcgtgcgat cgacgcgctg gcgcgcgcga tttcggtggc acgtgcggcc
     2581 gccgcatcga taccggcgtc gctggtgtcg gcgcttgccg cgaaccccca ggctccgtcg
     2641 aacagcgcgc gcacgccgaa tcccgtcgta ttaccgtcgg cgagcgtagc gacgacgccg
     2701 tttcgcactt cgatgcgctc ggagcgcgcg acctcgaaac gcacgtcggc atactgcgcg
     2761 ccgcgcgagg ccgccgtatc gagcgcgcgt gcggcttcgg cttcgaactc cataaattct
     2821 aaacgatgct cctattgacc ggcgatcacg gcgcggacgt tccgcacctc gatttgaccg
     2881 ctggaaagct gcatgccttt cggcggaaga acgtaaatgg cgattaggcg cgttatttcc
     2941 gccccaggag gaaagcggac gacgacgtgc cgccatccgg gccggtcgag ccgcgtcgcg
     3001 tcgaggagca c
//
LOCUS       DASUCJ010000218         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_659295, whole genome shotgun sequence.
ACCESSION   DASUCJ010000218 DASUCJ010000000
VERSION     DASUCJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659295"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFU90_02705"
     CDS             complement(<1..229)
                     /locus_tag="VFU90_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478710.1"
                     /translation="MLKRISLALFLAATTVAAQQTQPPNYKEKVDVNLVLLDAVVNDS
                     RGNQILGLDKDDFVVKENGVEQPVSSVDYFTN"
     gene            268..903
                     /locus_tag="VFU90_02710"
     CDS             268..903
                     /locus_tag="VFU90_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEU5478711.1"
                     /translation="MKPLLATLLVTFCAALANAQRVEFFVDGEGVRRSGVTSDFASGA
                     TRFEPQFRTGGGAGGGVNFFLSDRVSIEAKLAALATKTRVRIVGSDFIGTADLGWTQL
                     YPISAVLQWHLAERGAVRPYIGAGVVHTVLRNINKQVGAGATGIRFKDPTGLVLDGGL
                     EIAVARKWSLLGDARYVPIETRSRATFPGTTSALDMRVRPLIVSFGLGYRF"
BASE COUNT          161 a          304 c          344 g          203 t
ORIGIN      
        1 ggttggtgaa gtaatcgacg gagctgaccg gctgctcgac gccgttctcc ttcaccacga
       61 aatcgtcttt gtcgaggccg aggatctggt tgccgcgcga atcgttgacg acggcgtcga
      121 gcaggaccag attgacgtcc actttctctt tgtagttcgg cggttgcgtt tgctgcgcgg
      181 cgacggtcgt cgcggcgagg aaaagtgcga ggctgattct tttcaacatg ttggctacaa
      241 cgcacgcttc ttgctgaaat ttcgtgcatg aagccgcttc tggcaacgct tctggtgacc
      301 ttctgcgcgg ccctggcaaa cgctcaacgc gtggaatttt tcgtcgacgg cgagggagtc
      361 agacgcagcg gtgtgacgtc cgatttcgca tccggggcca cgcggttcga gccgcagttc
      421 cgcacgggcg ggggcgcggg aggcggtgtc aatttctttc tgagcgatcg cgtgtcgatc
      481 gaagcgaaac tcgccgcgct ggccacgaag acccgcgtgc gcatcgtcgg cagtgacttc
      541 atcggcaccg cggacctcgg ctggacgcag ctctatccga tatccgcagt gctgcagtgg
      601 catctcgcgg agcgcggcgc ggtgcggccg tacatcggag cgggcgtcgt gcacacggtg
      661 ctgcgcaaca tcaacaaaca ggtgggcgcc ggcgcgaccg gaatccgctt caaggatccg
      721 accggactgg ttctcgacgg cggtctggag atcgccgtcg cgcggaagtg gagtcttctc
      781 ggcgacgcgc gctacgtgcc gatcgagacg cgctcgcgtg cgacgttccc cggaacgacg
      841 tcagcgctgg acatgcgcgt ccgtccgctg atcgtttcct tcggtttggg ttatcgtttc
      901 tgaccgcgct gcggaatggc agtgtagggc acggctccgc cgtgccggac ttcgggtgcg
      961 gcagcgcgca gcgccgccct acagacgcgg aatgaactgt ccggccccga gg
//
LOCUS       DASUCJ010000219         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1500035, whole genome shotgun sequence.
ACCESSION   DASUCJ010000219 DASUCJ010000000
VERSION     DASUCJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500035"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFU90_02715"
     CDS             complement(<1..671)
                     /locus_tag="VFU90_02715"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU5478712.1"
                     /translation="MRFAPCVTIAVWLTVIGVSSARCQVDTRWPQHSMDRPRPPVVDP
                     GPERAPVPPPADAIVLFDHGSLSAWRQHDGTPARWAVHGDAFEVEPGTGNIMTARAFG
                     DCQLHVEWAAPVPPQGESQERGNSGVFLMGLYEVQVLDSYHNDTYPDGQAAAVYGQFP
                     PLVNASRPPGQWQSYDIIFHRPRFDASGAVTSPARLTVFHNGVLVQDNVALSGPTANK
                     QRPPY"
     gene            822..>1211
                     /locus_tag="VFU90_02720"
     CDS             822..>1211
                     /locus_tag="VFU90_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478713.1"
                     /translation="MLISLLSFALLLQAPNTAAAAPVIRSDSTVYDGRSRQLDVAPPR
                     IDTTIVVDGVLDEAVWRRAARLTGFSQYRPVDGSPATDSTDVLVWYSSDAIYFGIRAY
                     EPHGSVVRATLADRDNIDADDNVQILLD"
BASE COUNT          196 a          418 c          387 g          210 t
ORIGIN      
        1 tgatacggcg gccgctgctt gttcgccgtc ggtccgctca gcgccacgtt gtcctgcacc
       61 aatacgccgt tgtgaaacac cgttaggcgc gcgggcgacg tcaccgcgcc gctcgcgtcg
      121 aatcgcggtc ggtggaagat gatgtcgtac gattgccact gtcccggcgg ccggctcgcg
      181 ttgactaacg gagggaactg gccgtacacg gccgcggcct gtccatcggg atacgtatcg
      241 ttgtggtacg agtcgagcac ctgcacctcg tacaggccca tgaggaacac gccgctgttc
      301 ccgcgttcct ggctctcgcc ctgtggcggc acgggcgcgg cccactcgac gtgcagttgg
      361 caatcgccga acgcgcgcgc ggtcatgatg ttgccggtac cgggctcgac ctcgaaggcg
      421 tcgccgtgta ctgcccaacg cgccggcgtg ccgtcgtgct ggcgccacgc cgacaacgaa
      481 ccgtgatcga acaacacgat cgcgtccgcc ggcggcggca ccggcgctcg ctcgggtccg
      541 ggatcaacga ccggcggtcg aggccggtcc atcgagtgct gcggccagcg cgtgtccacc
      601 tggcatcgcg cgctcgacac gccgatgacg gtgagccaca ccgcgatcgt gacgcatgga
      661 gcgaatcgca tcagggatgg gtctttgaga gaagcgtgcg agaaccgtcg aggctacctc
      721 tttatacggc gcagacatcc acgtgacaag tggcccgtgg accggcgcaa gaccgttgga
      781 gattcggacg cgtgcacgta acatctacgt ccggacatcg catgctcatt tctcttcttt
      841 cgttcgcgct gctcctgcag gcgccgaaca ccgccgccgc cgcccccgtc attcgatcgg
      901 acagcaccgt ctacgatgga cggtcgcgtc agctcgatgt cgcgccgccg cgcatcgaca
      961 cgaccatcgt cgtggacggt gtgctcgacg aagccgtgtg gcggcgcgcc gccaggctca
     1021 ccggcttttc gcagtatcgc cccgtcgacg gctctcccgc cacggattcg accgatgtgc
     1081 tcgtgtggta ctcgagcgac gcgatctact tcggcatccg ggcgtacgag ccgcacggca
     1141 gcgtcgtgcg tgcaacgctc gccgaccgcg acaacatcga cgccgacgac aacgtccaga
     1201 tcctgctcga t
//
LOCUS       DASUCJ010000220         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1041501, whole genome shotgun sequence.
ACCESSION   DASUCJ010000220 DASUCJ010000000
VERSION     DASUCJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041501"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFU90_02725"
     CDS             complement(<1..1420)
                     /locus_tag="VFU90_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5478714.1"
                     /translation="MIQHDVVVIGGGLAGMRAAVEAAEGGADVAIVSKMHPVRSHSGA
                     AQGGINAALGNREEDSTQAHTFDTVKGSDYLGDQDAVAAMCEDAPQQLIWLEHRGCIF
                     SRLPDGRIAQRPFGGAGTPRTCYSADVTGLVILHTLWEQLERFGVKVYEEYFCTALAI
                     EDGVGSGVVAYNMRNGLLELVTAKATIFATGGAGRMYLKTTNGYASTADGVGIAFKAG
                     LPLMDMEFMQFHPTTLKENGVLMTEGARGEGAYLLNADGERFMFKYAPNKGELASRDV
                     VSRAEWTEILEGRGVDGCVLLDMRHLGREKILERLPQIRELALDATGKDAVNEPIPIL
                     PGAHYTMGGIETDKWGATRVPGVYAAGECACVSVHGANRLGGNSLLETIVFGARSAKH
                     ALEYIKGSGAVKASEKTLQQERDRLGLLLSRSGGTRAPQLRRAMNETMSANTFIFRDA
                     QGLSKAVDDLMQVRREFDSGAFV"
BASE COUNT          273 a          539 c          527 g          310 t
ORIGIN      
        1 ttacgaacgc gccggagtcg aactcgcgtc gaacctgcat caggtcgtcg acggctttgg
       61 agagtccctg cgcgtcgcgg aagatgaagg tattcgcact catcgtctcg ttcatggcgc
      121 ggcgcagctg cggcgcgcgc gttcctccgc tgcgggaaag cagcaagccg agccgatcgc
      181 gttcctgctg gagcgttttt tcggaagcct tgaccgcgcc cgagcctttg atgtactcga
      241 gcgcgtgctt ggcggaacgc gctccgaaaa cgatcgtttc gagcagcgag ttgccgccga
      301 gccggttggc gccgtgaacg ctgacgcagg cgcattcgcc ggcggcgtag acgccgggta
      361 cgcgggtcgc tccccacttg tcggtttcga tcccgcccat cgtgtaatgc gctccgggca
      421 aaatcggaat cggctcgttt accgcgtctt ttccggtcgc gtccagcgcg agttcacgga
      481 tctgcggcag gcgttcgagg attttctcac gtccgagatg gcgcatgtcg agcaggacgc
      541 acccgtcgac gccgcggccc tcgagaatct ccgtccactc ggcacgcgac acgacgtcgc
      601 gagaagccaa ctcgccctta ttcggcgcgt acttgaacat gaaccgctcg ccgtcggcgt
      661 tgagcagata ggcgccctcg ccgcgcgcgc cctcggtcat caacacgccg ttttccttga
      721 gcgtcgtggg gtggaactgc atgaactcca tgtccatcag ggggagtccg gctttgaagg
      781 cgatcccaac gccgtcggcg gtggaggcgt acccgttggt cgtcttgagg tacatgcgtc
      841 cggccccgcc ggtggcaaag atggtggctt tggccgttac gagctcgagg agaccgtttc
      901 gcatgttgta ggcgaccacg ccggaaccga cgccgtcctc gatcgcgagc gccgtgcaga
      961 agtactcttc gtagactttg acgccgaagc gctccaactg ctcccaaagc gtgtgcagaa
     1021 tgaccaagcc ggtgacgtcg gccgagtaac aggttcgcgg cgtgcccgct ccgccgaagg
     1081 gccgctgcgc gatccgcccg tcgggcagac gggagaagat gcagccgcgg tgttcgagcc
     1141 agatgagctg ttggggagca tcctcgcaca tcgcggcgac cgcgtcctgg tcgcccagat
     1201 aatcggagcc cttgacggtg tcgaaggtat gcgcttgggt cgagtcctcc tcgcggtttc
     1261 cgagcgcggc attgatgccg ccctgcgccg cgcccgagtg gctgcgcacc gggtgcatct
     1321 tggaaacgat ggcgacgtcg gccccgcctt cggcggcttc gaccgcggcg cgcatgccgg
     1381 ccagaccgcc gccgatgacg actacgtcgt gttgtatcaa atgaggccct tccgggaaga
     1441 tctcgtaact ctgctaccct atactatgcg cgcccaccgg cgcgccccat gactttagga
     1501 gcttggcccc ccgggggagc gcttgccgaa gaccgaactc ctggcctcgg caaacgtgcg
     1561 tcccatcaaa ggctgcctag gaaagcccgc ttgaaccagt tcgtccacct tcacgtacac
     1621 tcggaatact cgctcctgga cggagcctg
//
LOCUS       DASUCJ010000221         5454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_567852, whole genome shotgun sequence.
ACCESSION   DASUCJ010000221 DASUCJ010000000
VERSION     DASUCJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567852"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VFU90_02730"
     CDS             <1..1284
                     /locus_tag="VFU90_02730"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF004856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU5478715.1"
                     /translation="KLIARALGAYCWDVDGNRFIDWAMGNRVMVLGHANPAVNDAVKR
                     QIDLGVNFTRPGVLEYELAEYLVDLLPVAEMVKFGKNGSDVTTAAIKLARAYTNRKMV
                     AVCQEHPFFSIHDWFIGTTPMDAGVPPEVAKLTVRFNYNDIASLQALYDRYPGQIAAV
                     ILEPVKNDEPRNNFLEDLRELTRREGSVLIFDEMISGLKFDLRGAHHRWKVYPDLACY
                     GKAMSNGYSFSCLAGRRDILELGGLKHDKQRVFLLSQTHTSETVGLAACRATLDEYQR
                     LNVGDHIWSQGAKLVSGIRELAKAEGVADHIRVIGFDCNPYILCTRADGTPWPALHTS
                     FHEEVISWGVLIPWVTITYSHGAEEIARTLEALQHGMRKIRRALDDGSVERSFLGEAV
                     KPVFRPFNRCCQSKCGRLYTDAPRLACCTDDGDDA"
     gene            1281..2084
                     /locus_tag="VFU90_02735"
     CDS             1281..2084
                     /locus_tag="VFU90_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEU5478716.1"
                     /translation="MIGAVVQARMGSTRLPGKVLMPAAGKPLLAHLLERLGRARMVET
                     MLVATSDDVRDDPVAALCSSLGVEVFRGSEHDVLDRYYQAARGAGIDLVVRITADCPL
                     VDPAIVDAMVQYAVEHRGEFDLVTNRRPLTFPDGLDLDVIPIDALAQAWREAREPMQR
                     EHVIPWFWDTGRRLHNLEYPANLYAEHRWTLDYAEDYALIRRVIEELTPVFGSTFAME
                     DVLAFMADHPDVAHSNAQYITTQPVASTGRILSMAAAGRAQPIASSRSA"
     gene            2088..4307
                     /locus_tag="VFU90_02740"
     CDS             2088..4307
                     /locus_tag="VFU90_02740"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEU5478717.1"
                     /translation="MPPSFDYTRDELIDALRASGLGPGDIVFAHIGLDALGRADGCLT
                     DDDASAMLMKAFDAVLGPAGTLLVPTYTFSFCRQESFDVQHTTTHGGPWSTYAPFLEY
                     FRLLPDTVRSADPIHSVAGRGPMAAALLRDIPPTCFGPGSVFDRLHAAGAKVCTLGVG
                     LSEASFQHFVEESVGVPFRFKKLFTGVIRDNGQERKTGWVFNVRILGDQSEPHLTHLE
                     TAATAAGICRVANVGLDAIRTAGCQPLRELILRNLARDPWFTARGPAADPIALETQRV
                     PPRRFDIHMPESATIPQMIDALWRVPRDIVSDGYDAALAALATQVPMTVHEYPSGSEC
                     WSWIVPEKWTCREAYLETLDGRRLFSYSDHPLHVVSYSLPYHGVVSRDELLRHLHVDE
                     KQPDAVPFIFKYYERDWGLCCSKTLRDSLTDAEYRVVIDTSFSYGTLKAGEVIARGAS
                     DETIVLCAHLCHPGMVNDDLTGVVVGIDVMRALLARRDLRYTYRFVIIPETIGTVSYL
                     SRNEDLIPHLKGGLFLEMLGLDHPHALQLSFTGSTEVDDCFTAALRAGDPEGWTGAFR
                     TVIGNDERQYNAPGVRVPMLSLSRVLRPDSPNWPYRGYHSTNDTPEITSNERLAASRD
                     LVLRMIDALEANVIPVNVYKGEVFCSRFGVHVDFYTNPEGNKALFDILFLIDGTRSIA
                     RIAAECGISFEAARQTIEELHRRGVVRLDGPAASVEPARKAARATTEHRGTARRQPA"
     gene            4689..>5454
                     /locus_tag="VFU90_02745"
     CDS             4689..>5454
                     /locus_tag="VFU90_02745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEU5478718.1"
                     /translation="MNCLAIIPARGGSKGVPRKNIRLLAGHPLLSYSVAHARATPEIN
                     RIVVSTDDPMIARVAHECGAESIVRPAELSGDTATSESALLHTLDHLRDTEDYEPDLV
                     VFLQATSPLRRADDISMAIGTLLDEAADSLFSACAMHGFVWRRDRGGSEWHSQTYDYR
                     NRQRRQDAPEDVVENGSIYVFRPWVLRELGNRLGGKIAVHFMEPAESVQVDTPSDLEL
                     VEHLLSRRRAPASAAELGRVRLLALDFDGVLTDNRVV"
BASE COUNT          955 a         1847 c         1694 g          958 t
ORIGIN      
        1 aagctgatcg cgcgcgcgct tggcgcctac tgctgggacg tcgacggcaa ccgcttcatc
       61 gactgggcca tgggcaaccg cgtcatggtg ctcggtcacg cgaacccggc cgtcaacgac
      121 gcggtcaagc ggcaaatcga tttgggcgtg aacttcaccc gccccggcgt tctcgagtac
      181 gagttggccg agtacctcgt cgacctccta cccgtcgccg agatggtgaa gttcggcaag
      241 aacggctcgg acgtgactac ggcggcgatc aagctcgcgc gcgcgtacac gaaccgaaag
      301 atggtcgccg tgtgccagga gcacccgttc ttcagcatcc acgactggtt catcggcacg
      361 acgccgatgg atgccggcgt gccgcccgaa gtcgcgaagc tcaccgttcg cttcaactac
      421 aacgacatcg cgagcctcca ggcgctctac gatcgctatc cgggccagat cgccgcggtg
      481 attctcgagc cggtcaagaa cgacgagccg cgcaacaact tcctcgagga ccttcgcgag
      541 ctcacgcgtc gtgaagggtc ggtgctgatc ttcgacgaga tgatcagcgg cctcaaattc
      601 gacctgcgcg gcgcacatca ccgctggaag gtgtatcccg acctcgcctg ctacggcaag
      661 gcgatgagca acggctattc gttctcgtgc ctcgccggcc gccgcgacat tctcgagtta
      721 ggcggattga agcacgacaa gcagcgcgtg ttcctgctct cgcagaccca tacgtccgaa
      781 acggtgggcc tcgccgcctg ccgcgcgacg ctcgacgaat accagcgcct gaacgtcggc
      841 gatcacatct ggtcgcaggg cgcgaagctc gtctcgggaa ttcgcgagct ggcaaaggcg
      901 gaaggcgtcg ccgaccacat tcgcgtaatc ggcttcgatt gcaatcccta cattctgtgc
      961 acgcgcgccg atggcacccc gtggcccgcg ctgcatacgt ccttccatga ggaagtgatt
     1021 tcgtggggcg tcctcattcc gtgggtcacc atcacctact cgcacggcgc cgaagaaatt
     1081 gcacgcacgc tcgaggcgct gcagcacggc atgcgcaaga ttcgtcgcgc actcgatgac
     1141 ggttcggtag agcggtcgtt cctgggcgaa gccgtgaagc cggtattccg cccgttcaac
     1201 cggtgctgtc agagcaaatg tggacggctg tacaccgatg cgccgcgcct tgcatgctgc
     1261 accgacgacg gcgacgacgc atgatcggcg cagtcgtcca ggcgcgcatg ggttccaccc
     1321 ggctgcccgg caaagtactc atgccggcag ccgggaagcc gctgctcgcg cacctgctcg
     1381 agcggctggg ccgcgcccga atggtcgaga cgatgctcgt cgccacgtcg gacgacgtgc
     1441 gcgatgaccc cgtcgccgcg ctgtgctcgt cgttaggcgt agaggtcttt cgcggcagcg
     1501 agcacgacgt cctcgaccgc tactaccagg ccgcgcgcgg cgccggcatc gacctcgtcg
     1561 tccggattac cgccgactgc ccgctcgtcg atcccgcgat cgtcgacgcg atggtgcagt
     1621 acgcagtcga gcaccgcggc gagttcgatc tcgtcaccaa tcgacgcccg ctcaccttcc
     1681 cggacggatt ggatcttgac gtcatcccga tcgacgcgct cgcccaagca tggcgcgagg
     1741 cgcgcgagcc catgcagcgc gaacacgtca ttccatggtt ctgggatacg ggtcgccgcc
     1801 tgcataacct cgagtatccg gccaatctgt acgccgagca ccggtggacg ctcgactacg
     1861 ccgaggacta cgcgctcatc cgtcgcgtga tcgaggagct gacgccggtg tttggatcga
     1921 cattcgcgat ggaagacgtg ctcgccttca tggccgacca cccggacgtc gcgcattcga
     1981 acgcccagta catcaccacc caaccggtgg cgtccacggg acgcatactc tccatggcgg
     2041 ccgccgggcg cgcccaaccg atagccagca gccgcagcgc ctgacccatg ccaccatctt
     2101 tcgattacac gcgcgacgag ctgattgacg cgctccgtgc ctcggggctc ggccccggcg
     2161 acatcgtctt cgcccacatc ggtctcgacg cactcggccg tgccgacggt tgcctaacgg
     2221 acgacgatgc cagcgccatg ctcatgaagg ccttcgatgc cgtgctggga cccgccggca
     2281 cgctcctcgt gcccacctac acgttctcct tctgccggca ggaatcgttc gacgtccagc
     2341 acacgacgac gcacggcgga ccgtggagca cgtacgcgcc gttcctcgag tacttccggc
     2401 tgctgcccga cacggtgcgc tccgccgacc cgattcactc ggtcgccgga cgcggcccca
     2461 tggccgccgc actcctccgc gacattccgc ccacgtgctt cggccccggc agcgtgttcg
     2521 accgcttgca cgccgccggc gcgaaggtct gtacgctcgg agtcggcctc agcgaagcca
     2581 gctttcaaca ctttgtcgaa gagtcggtcg gcgtcccgtt ccgcttcaag aaactgttca
     2641 ccggtgtcat tcgcgacaac ggacaggagc ggaaaaccgg ttgggtgttc aacgtcagga
     2701 tcctcggcga tcaaagcgag ccgcacctca cacatctgga gacggccgcg acggcggcgg
     2761 gcatctgccg cgttgctaac gttgggttgg acgcgattcg cacggccgga tgccagccgc
     2821 tgcgcgagtt gattctgcgc aacctcgcac gcgatccgtg gttcaccgcg cgcggaccgg
     2881 ccgccgatcc gatcgcactc gagacgcaac gcgtgccgcc ccgtcgcttc gacatccaca
     2941 tgccggagtc ggcgaccatt ccgcagatga tcgacgcgct gtggcgcgtg ccccgcgaca
     3001 tcgtctccga cggctacgat gcggcgctcg ccgcgctcgc cacgcaggtg ccgatgacag
     3061 tgcacgaata tcccagcggc agcgagtgct ggagctggat cgtgccggaa aaatggacgt
     3121 gtcgcgaggc gtatctcgag acgctcgacg ggcgccgcct cttttcctac agcgaccacc
     3181 cgctgcacgt cgtgtcgtat tcgctgccct atcacggcgt cgtctcgcgc gacgagctgc
     3241 ttcgacacct ccacgtggat gagaagcagc ccgatgccgt gccgttcatc ttcaagtact
     3301 acgagcgcga ctggggtctc tgctgcagca agacgctgcg cgattcgcta acggacgccg
     3361 aatatcgcgt cgtcatcgac accagcttca gttacggcac actgaaggcc ggcgaagtga
     3421 tcgcgcgcgg cgcgagcgac gagaccatcg tgctgtgcgc gcacctgtgc cacccgggca
     3481 tggtcaacga cgacctcacg ggtgtcgtcg tcggcatcga tgtcatgcgc gcgctcctgg
     3541 cgcgccgcga tctgcgctac acctatcgtt tcgtcatcat tccggagacg attggcacag
     3601 tctcgtatct gagtcgcaac gaagacctga tcccgcacct caaaggcggc ttgttcctcg
     3661 agatgcttgg cctggatcac ccacatgcgc tgcagctctc gttcacaggc agcaccgagg
     3721 tcgacgattg cttcacggct gcgctgcgcg ccggcgaccc ggaaggctgg acgggcgcat
     3781 ttcgcactgt gatcggcaac gacgaacgac aatacaacgc gcccggcgtg cgcgtcccga
     3841 tgttgtcgct gtcgcgcgtg ctgcgccccg actctccgaa ctggccgtat cgtggatatc
     3901 acagcacaaa tgacacgccg gagatcacgt ctaacgaacg gctcgctgca tcgcgcgacc
     3961 tcgtgctgcg catgatcgat gcgctcgagg cgaacgtgat tcctgtcaac gtctacaagg
     4021 gcgaagtctt ctgctcgcgt ttcggcgtgc acgtggactt ttacacgaat ccggaaggca
     4081 acaaagcgct cttcgatatc ctgttcctca tcgacggtac tcgctcgatc gcgcgcatcg
     4141 ccgcggagtg cggaatctcg ttcgaggccg cgcgtcagac catcgaagag ctgcatcggc
     4201 gtggcgtcgt ccgtctcgac ggcccagccg cgagcgtcga gccggcgaga aaagccgcgc
     4261 gcgcgactac agagcaccgc ggtacggctc gtcggcagcc tgcttgatcc tggaggcacg
     4321 ctgccgacgg tatcgatgac gccgcgcatc ggtgttgatt ggtcgcaaca gagaacagta
     4381 cactcgtcgg cgcaatgata tcgtgagcgg tgtggatcag gcatataagt cgttatgcag
     4441 caataggatg atttgtgagg cttcagtcga cgaaagggtg ccttctcgtg cccgcatctt
     4501 gccccgcctc gggcgcgcac accgacgcga ctcgcagctg gagacggtct cggttcggta
     4561 cgcatcgccg taccgcgcta tcctgaaaac cgaggatggc aacacagagc gatgaatgca
     4621 ccgtcgtccg atcgtggcag cctcatgtat gatggacgtc ttcgcacggg atcttcggtg
     4681 agcacgcgat gaattgtctg gccattatcc ccgcccgcgg cggatcgaaa ggcgtaccca
     4741 ggaaaaacat ccgcctgctg gccggccatc cgctgctctc gtacagcgtc gcgcacgcgc
     4801 gcgcgacgcc ggagatcaat cgcatcgtcg tgtcgacgga cgatcccatg atcgcgcgcg
     4861 tcgcccacga atgcggcgcc gagtcgatcg ttcggcctgc ggaactcagc ggcgatacgg
     4921 cgacgagcga gtccgcgctc ctgcacacgc tcgaccacct gcgcgacacc gaggactacg
     4981 agccggatct cgtggtgttc ctccaggcca cgtcgccgct gcgccgcgcc gatgacatct
     5041 cgatggccat cgggacgctg ctcgacgaag cggccgactc gctgttttcc gcgtgcgcga
     5101 tgcacggctt cgtgtggcgg cgcgaccgcg gcgggagcga gtggcactcg cagacgtacg
     5161 actaccgcaa tcggcagcgg cgccaggatg cgcccgaaga cgtcgtcgag aacgggtcga
     5221 tctacgtctt ccggccgtgg gtgctgcgcg agctgggtaa ccggttaggc ggaaagatcg
     5281 cggtgcattt catggagccg gccgagtcgg tacaggtcga cacgccgtcg gatctcgagt
     5341 tggtggagca cttgctgtcg cgccggcgcg cccccgcgag cgcggcggag ctcgggcgtg
     5401 tgcgcttgct cgctctcgac ttcgacggcg tcctaacgga caaccgcgtg gtcg
//
LOCUS       DASUCJ010000222         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_506748, whole genome shotgun sequence.
ACCESSION   DASUCJ010000222 DASUCJ010000000
VERSION     DASUCJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506748"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..793)
                     /locus_tag="VFU90_02750"
     CDS             complement(83..793)
                     /locus_tag="VFU90_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478719.1"
                     /translation="MLLATAAAFVITERLKLTPSPIAGTRVSKTFSPVCGCATDNASV
                     DFRLRRGGLLQVDVITRGGTVVRRLARRRFQRGWLSFRWFGHDQSGRPSPDGDYRIRV
                     HMPSEHRTIVFPNTIRLDTVAPKIEQFRISRRAIRIGERTRALYRFGDKAHPILLVDG
                     KAAVYGRFAHTSGNVDWFGKVGALPVRPGIHRLALEARDEAGNTSATTGSIRVRVKAR
                     PAKRRTHLRTAKRHAPHG"
BASE COUNT          235 a          456 c          472 g          229 t
ORIGIN      
        1 cgcggagcat gccagtccga ttgcgagaag ggctgtgaaa gcgaaccaac gcagcatccg
       61 acgcttctcg cgcgctcggc ggtcagccat gcggcgcgtg tcgtttcgcg gttcggaggt
      121 gagtgcgccg cttcgccggc cgcgccttca cccggacgcg gatgctgccc gttgtggcgg
      181 aagtgtttcc ggcttcgtcg cgggcctcga gtgcgagccg atgaatgcct gggcgaacgg
      241 gtagcgcacc aacttttccg aaccaatcca cgttcccgga cgtatgcgca aagcggccgt
      301 agacggcagc tttgccgtcg acgagcagga tcgggtgcgc cttgtcgccg aagcgataca
      361 gcgcacgggt gcgctcgccg atacggattg cgcgacgcga gatgcggaac tgctcgatct
      421 tcggggcgac cgtgtccagg cggatcgtgt tggggaacac gatcgtgcgg tgctctgaag
      481 gcatgtggac gcggatccgg tagtcgccgt cggggctcgg ccggcccgat tggtcgtgtc
      541 cgaaccagcg aaaagacagc cacccgcgct ggaatcgacg tcgcgcaagc cggcgcacca
      601 ccgttccgcc ccgcgtaatg acgtccacct ggagcaatcc gccgcggcgc aggcgaaagt
      661 cgacggatgc gttgtcagtc gcgcagccac acaccggtga gaacgtcttc gacactcgcg
      721 tgccggcgat cggagagggc gtcaacttca gccgctcggt aatcacgaac gccgccgcag
      781 tcgccaggag caagccgccg atagtggcaa cggcaaggag gcgctgaggg cgaccagttc
      841 cccgcggcga tcggtgtgcg tcatcgtcgc ccggaggagg gccgacaccc gcgccgtcgg
      901 tcatcgctcg agaccgcaca gaaatgggct tgggcgcacc gtcgaatctg gcagcacgcg
      961 ggcgaacgct ccaggaagct gctgacgttc ggcagagagc gacatcgcgc acgcacctcg
     1021 gttccaccca cgaatggcgc ctgcgccgaa tgcatcatgc cttgaccgac aacgccgtgt
     1081 gcgtgcaagc gatcgcggcg tccgttctcc gatccacatt cgctgtaccc cggcatcctt
     1141 ggaccgggag cgtgggctcg cgaaccgggc tggcgcgcga gttactcgtt cgcgtggcag
     1201 cccggtaccg ctggatcagg ctctgacgtc aatcgcctga cggcgtgccg cctgcgaggc
     1261 cgcgtcacca tcgttgaggg gccgctgcgc cagtgcgacc gtcatcgtcc gcttccttgt
     1321 agacagcgcg cgaaacgccg cttgagcggc atctcgtcca cttccgtgta gacagcgcgt
     1381 ggtatgtcgt ct
//
LOCUS       DASUCJ010000223         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1408315, whole genome shotgun sequence.
ACCESSION   DASUCJ010000223 DASUCJ010000000
VERSION     DASUCJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408315"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFU90_02755"
     CDS             <1..312
                     /locus_tag="VFU90_02755"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEU5478720.1"
                     /translation="VVVHADDDGKTFDLAVGSRIAFALANHSGTGFVWMPTRVDESVL
                     APDGMRRSEIESDVPGAPKRDVLRYVAKKAGSTVVEMTLKRPFGTAPPGPAIHVTVRV
                     H"
     gene            622..1011
                     /locus_tag="VFU90_02760"
     CDS             622..1011
                     /locus_tag="VFU90_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478721.1"
                     /translation="MFVPAIAFAWWLVALPMKSLPAVVLQSGSQTSAAPAHPRRAQAK
                     APALHADADADDDTPTSERIPLRGPIPADVVHAARSFLDLPLGAERRARIGDTDYLFV
                     LEWHYHPPGFVGGPNGWHKGVTVYLTR"
BASE COUNT          179 a          396 c          368 g          184 t
ORIGIN      
        1 gtcgtcgtcc acgcggacga tgacggcaag acgttcgacc tcgcggtcgg ttcccgcatc
       61 gcgttcgcgc tcgcgaacca ttcgggaacg ggcttcgtct ggatgccgac ccgcgtcgac
      121 gagagcgttc tcgcgccgga cgggatgcgc cgcagcgaga tcgaatcgga cgtccccggc
      181 gcgccgaagc gcgacgtctt gcgctacgtc gcgaagaaag ccgggtcgac ggtcgtcgag
      241 atgaccctga agcggccctt tggcaccgca ccgccgggcc cggcgatcca cgtcacggtg
      301 cgcgtccact gatcacagga gtcgcgcgcc gcgtgacccg cgcgccgcgt gagccgcgcc
      361 cgccgcgccg gtgtgcctaa gctgtaccta gaaagcgcca aaaatagcag aatcggctga
      421 ggtctgaaga ctgatacctt ttgtcctcac cttcgcctta cgcacctcgg tggggcaagg
      481 tgcgcacgcc aagtaacgct ctgatctcgc aacagttctg cggcattgac gcgacttggc
      541 acgattcgac cataagccgg gcctctcgcc tgcgtacagc cggccgcggt gacggcgacg
      601 gcgagaccga aagaggtgcc catgttcgtg cccgcaatcg cattcgcctg gtggctcgtc
      661 gcccttccga tgaagtctct gcctgccgtc gtgctgcaaa gcggctcgca gacttccgcc
      721 gcccccgcgc atccgcggcg cgcgcaagcg aaggccccgg cgctgcatgc ggatgccgac
      781 gcggacgacg atacccccac gagcgaacgc atcccgctcc ggggccccat cccggccgac
      841 gtcgttcacg cggcgcggtc gttcctcgat ttgccgctgg gcgcggagcg acgcgctcgc
      901 atcggcgaca cggattattt gttcgtgctc gagtggcact accacccgcc cggcttcgtc
      961 ggcggcccga acggctggca caagggcgtg acggtctacc tcacgcggta aggcacttca
     1021 cacggtcacg aagccgtttc acgcgcggct tcggctccag cggtgcttga tcccgggcgg
     1081 gaagtagtcc gggtcgcccg cggcgcggag ctcgatcgaa ggcgttt
//
LOCUS       DASUCJ010000224         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_736035, whole genome shotgun sequence.
ACCESSION   DASUCJ010000224 DASUCJ010000000
VERSION     DASUCJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736035"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFU90_02765"
     CDS             <1..306
                     /locus_tag="VFU90_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5478722.1"
                     /translation="MNDHWKNGKVSTEAAILGAGERLRPILMTACAMTVGMVPMALAL
                     ERGSQMQAPLGRAVIGGLAVATVATLFFVPTVFSVLHRRHGAGERAVESVELSTPAT"
     gene            520..>1176
                     /locus_tag="VFU90_02770"
     CDS             520..>1176
                     /locus_tag="VFU90_02770"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5478723.1"
                     /translation="MAKWTADQAIPEVSVIIPQRGVSGTQLVLPGNVKAWFQAPIYSR
                     VSGYVKQWYFDFGAHVKQNQVLALIDTPDLDAQFGAAEADLDAARAKVNIAKAAMEFA
                     KTTYDRWRDSPKGVVSMQETESKKADYETALASYDAAIAGVKSDEGVVNRLRALEQFK
                     RIVAPFDGVVTERNTDIGDLINAGSGAGGGSAPTLFQVADVHRMRVFVQVPQAMSAQI
                     T"
BASE COUNT          219 a          355 c          405 g          197 t
ORIGIN      
        1 atgaacgatc actggaagaa cggcaaggtc tcgaccgagg cggcgatttt gggagccggc
       61 gagcggttgc ggccgattct gatgaccgcg tgtgccatga ccgtcggcat ggtgccgatg
      121 gcgttggcgt tggagcgcgg cagccaaatg caggcgccgc tcggtcgcgc ggtgatcggc
      181 ggactggccg tcgcgacggt cgcgacgctt ttctttgttc ccaccgtttt cagtgtccta
      241 catcgcaggc acggtgcggg cgagcgtgca gtagagagcg tcgagctctc gacgcccgca
      301 acctgacgca cgaggagatt cgctttgatc gagacagacg atcccggtga ggcgctgcag
      361 tccgtgaggg atgcgccgga tagctcggtg aaccgaggac ggccgcctgc agccgccatc
      421 ccgactgcga ggctgcgacg cattggattc gcagccggcg cgatcgcgat cctcatcgcc
      481 gcgatcggta tcgccgatcg ggaaatcaac gagcgttcag tggcgaagtg gactgcagat
      541 caagcgatcc cggaggtttc ggtcatcatc ccgcagcgcg gcgtatcggg cacgcagctc
      601 gtgctgcccg gcaacgtgaa ggcgtggttc caagcgccga tctactcgcg cgtgagcggc
      661 tatgtgaagc agtggtactt cgattttggc gcgcacgtga agcagaatca ggtcctcgcg
      721 ctgatcgaca ctcccgacct cgatgcccag ttcggggccg ccgaggccga tctcgacgcc
      781 gcccgggcga aagtcaacat cgcgaaggcc gcgatggagt tcgcgaagac gacgtacgac
      841 cggtggcgcg actcgccgaa aggcgtcgtc tcgatgcagg agacggagtc caagaaggcg
      901 gactacgaga ctgcgctggc gagctacgat gccgccatag caggtgtgaa atccgacgag
      961 ggagtcgtca atcggttgcg cgcactcgag cagttcaaac gaatcgtcgc cccgttcgac
     1021 ggcgtcgtga cggagcgcaa cacggacatc ggcgacctca tcaatgccgg cagcggcgca
     1081 ggcgggggga gcgccccaac gctgtttcaa gtcgccgacg tgcatcgcat gcgcgtcttc
     1141 gtccaggtgc ctcaggcgat gtccgcgcag atcacg
//
LOCUS       DASUCJ010000225         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_491375, whole genome shotgun sequence.
ACCESSION   DASUCJ010000225 DASUCJ010000000
VERSION     DASUCJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491375"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1042
                     /locus_tag="VFU90_02775"
     CDS             <1..>1042
                     /locus_tag="VFU90_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5478724.1"
                     /translation="HREDRDGTFDEAQVLWKKRNGAPIWVQINEHTIHGPHGETMFEG
                     FVYDITERQLAEQQVSAANVQRKAVLDAATGVSIIATDAEGVITVFNTGAERMLGYSA
                     SEVIGRHSVIDLHRAEELEYHANRLQSEFGYRLSGFDILAHRADREGFEEREWTYVKR
                     DGSTITVLLSVTALRRDDGWLTGFLHVANDVTERKRAEETLQKQSAAMTASMDGIGIV
                     NNRLEFTVVNDALAKLYGYSDPQQLVGLSLHTLYEPQTYERFTSTILPIVKQRGRWRG
                     EAPGLRHDGLTFPQEISISAIEGGGLVCVVRDITERTYAEEQIKHLAYHDALTGLPNS
                     LLFKDRLTVAISH"
BASE COUNT          222 a          351 c          316 g          153 t
ORIGIN      
        1 tcaccgcgaa gaccgcgacg gaacgttcga cgaagcgcag gtgctctgga aaaagaggaa
       61 cggcgcgccg atctgggtgc agatcaacga gcacacgatc cacggcccgc acggcgaaac
      121 gatgttcgaa ggcttcgtct acgacatcac cgagcgccag ctggccgaac agcaggtctc
      181 cgcagcgaac gtccagcgca aagcggtcct cgacgccgcc accggcgtgt cgatcatcgc
      241 caccgacgcg gaaggagtca tcaccgtctt caacacgggc gcggagcgca tgctcggcta
      301 cagcgcctcg gaagtgatcg gccggcattc ggtgatcgac ctccaccgcg ccgaagagct
      361 cgaataccac gcgaaccgtc tgcaatcgga gttcggatac cggctcagcg gattcgacat
      421 cctcgcccat cgcgccgatc gcgagggatt cgaagagcgc gagtggacgt acgtgaagcg
      481 cgacggcagc acgatcaccg ttctgctctc ggtcaccgcc ctgcgccgcg acgacggctg
      541 gctgaccggc ttcctgcacg tggccaacga cgtcaccgaa cggaagcgtg ccgaggagac
      601 gctgcagaag cagtccgcgg cgatgacggc gtcgatggac ggcatcggca tcgtcaacaa
      661 ccggctcgaa ttcaccgtcg tgaacgacgc gctcgcgaag ttgtacggat actccgatcc
      721 gcaacaactg gtcggcctgt cgctgcacac gctgtacgaa ccgcaaacgt acgagcgttt
      781 caccagcacg attctgccga tcgtcaagca gcgcggccgc tggcgcggtg aagcccccgg
      841 cctgcgccac gacggactca cattcccgca ggaaatctcg atcagcgcga tcgaaggcgg
      901 cggcctggtt tgcgtcgtcc gcgatatcac cgagcgcacg tacgccgaag agcagatcaa
      961 acatctcgcg tatcacgacg cgctcaccgg cctgccgaac agtctgctgt tcaaagaccg
     1021 gctgacggtt gccatctcgc ac
//
LOCUS       DASUCJ010000226         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1255603, whole genome shotgun sequence.
ACCESSION   DASUCJ010000226 DASUCJ010000000
VERSION     DASUCJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255603"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..591)
                     /gene="lspA"
                     /locus_tag="VFU90_02780"
     CDS             complement(100..591)
                     /gene="lspA"
                     /locus_tag="VFU90_02780"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5478725.1"
                     /translation="MSRNRNSERAALVNTLIVAAVAIVVLWIDQYTKRLIVASFMPGE
                     SRFVIPHFLKWTYERNFHGAFGLFGSNAVLLIGMAIVVLVLFWFSFRDAAAKSRLVRV
                     AFGMIVGGAIGNVVDRVHFGYVIDFVDFYRIWPNIFNVADSCITVGVALLVLSSLVTR
                     RHR"
     gene            complement(569..>1165)
                     /locus_tag="VFU90_02785"
     CDS             complement(569..>1165)
                     /locus_tag="VFU90_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5478726.1"
                     /translation="EAATAARLQTLAHALAFVLGFSLVFVLLGLGASAIGGLLNAHRT
                     LISQIGGVMVVILGLQMMGMIRIPFLMMDKRAHVTRDGSRSFWTSLVVGMAFAAGWSP
                     CIGPILAGILAIASQQRNGEAALLLCFYSLGLAVPFLVTAAAVGAVLPALGRVKRFLP
                     AIEFAAGAFLIVVGLVLVNDAFLNVAGWFYQFVPQPQL"
BASE COUNT          266 a          384 c          368 g          147 t
ORIGIN      
        1 ccgacgcgga cgtcgccgcg gcgcaccgcc tcggccacca gcgaacgcga cgcacccgac
       61 aggcgggcga cgacgacgtc ggcgcgcttt ccggcttctt cagcggtgac ggcgtgtaac
      121 aaggctcgaa agaacgagca aggcgacgcc gaccgtgatg cacgagtcgg cgacgttgaa
      181 gatattcggc caaatgcggt aaaaatcgac gaaatcgatc acgtagccga agtgcacgcg
      241 atcgacgacg ttgccgatcg ccccgcccac gatcatgccg aacgcgacgc gcacgagacg
      301 cgatttggcc gcggcgtcgc gaaagctgaa ccaaaagagc acgagcacga cgatcgccat
      361 gccgatcagc aggacggcgt tgctcccgaa aagtccgaac gcgccgtgga agttccgctc
      421 gtacgtccat ttcaggaaat gcggaatgac gaaccggctc tcgcccggca tgaacgatgc
      481 gacgatcagc cgtttcgtgt actggtcgat ccagagtacg acgatcgcga cagccgcgac
      541 gatcagcgtg ttgacgagcg cggcgcgctc agagttgcgg ttgcgggaca aattgataga
      601 accatcctgc gacgttgagg aacgcatcgt tgactaatac caaccctacg acgatgagaa
      661 acgcaccggc ggcgaactcg atcgccggca agaagcgctt gacgcgaccg agcgcgggca
      721 gcaccgcgcc gaccgcggcg gcggtaacga gaaacggaac cgccaaaccc agcgaataga
      781 aacacagcag cagcgccgcc tcgccgttgc gctgctgcga cgcgatcgcc agtatgcccg
      841 cgaggatcgg gccgatgcag ggcgaccagc cggccgcgaa cgccatcccg accaccagcg
      901 acgtccagaa cgaccggctc ccgtcgcgcg tcacgtgcgc gcgcttatcc atcatgagaa
      961 acggaatgcg gatcatgccc atcatctgca gcccgagaat cacgaccatc acgccgccga
     1021 tctgggaaat cagcgtgcgg tgcgcgttga gcaagccgcc gatcgcgctc gcccccaggc
     1081 cgagaagaac gaacaccagg ctgaagccga gcacgaacgc gagcgcgtgg gccagcgtct
     1141 gcaggcgcgc cgcggtggcg gcttc
//
LOCUS       DASUCJ010000227         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_430233, whole genome shotgun sequence.
ACCESSION   DASUCJ010000227 DASUCJ010000000
VERSION     DASUCJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430233"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..860
                     /locus_tag="VFU90_02790"
     CDS             156..860
                     /locus_tag="VFU90_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5478727.1"
                     /translation="MPARTGRRRQSAGLLRSDQPTQPRLRAAIVATARKMNALGINRG
                     TAGNVSVRSKDGFLITPSAMAYDAMKPADVVAVDANARARGLREPSTEWRFHHAIYVA
                     RPEVGAIVHTHSVFATALACLDRSIPAFHYMVAKAGGNDIRCAPYATFGTQELADEAV
                     RALDGRQACLLSHHGMIAVGADLDAALAMAVEVEWLAETYCRVLQIGNSSILDADEIA
                     RNRMQFLDYGTQRRSR"
     gene            complement(943..>1917)
                     /locus_tag="VFU90_02795"
     CDS             complement(943..>1917)
                     /locus_tag="VFU90_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478728.1"
                     /translation="RLAEKLRLVDQIVGVAAETNDGSTVTSEQLRWLREALYMLPLAE
                     VQAIGVPGGFASAAAAVSSMQRSPRVAKALGSATTDLVYVPITPCRYIDTRVKPPMVG
                     STPYGVDLGQTGASYGGSAGCNIVTAAGLSNASDIAAVNMNVGIVNPQFQPGFLGARP
                     AGSANSTSMVNWYQSGPTVQASNAGAVTTSGAATGSNQIEFFGTPTDLVVDIFGVFAR
                     PGATALNCTFASLNGTGSGNVATGSNYTDLAVAQACPTGYAAVGLGCVYGPVAPAGLA
                     LISKSIGVAPTELSQPGFLGCVWFNGSTGALNGTDFHSYTRCCQVPGR"
BASE COUNT          333 a          630 c          646 g          308 t
ORIGIN      
        1 tcgcgccggc gcggacgcgc gcggtcaatt acgcattcga cgtgaccccg gccaggctcg
       61 tcaccggcta catcaccgag cgcggcatcg cgcgcaatgc gcaggagctc gcggctatgc
      121 atcccgagcg cgtgcgcagg cggggaagct gaccgatgcc tgcgcgtacc ggaagacgca
      181 ggcaatcggc ggggctcttg cgctcggacc agccgacgca gccgcggctt cgcgccgcga
      241 tcgtcgcgac cgcgcggaag atgaacgcac tcggcatcaa ccgcggcacc gccggcaacg
      301 tgagcgtccg gtcgaaggac ggatttctga tcacgccgag cgcgatggcc tacgatgcga
      361 tgaagcctgc ggacgtcgtc gccgtcgacg cgaacgcccg cgcgaggggc ttgcgcgagc
      421 cttcgaccga gtggcgcttc caccacgcga tctacgtcgc gcggccggag gtcggcgcga
      481 tcgtccacac gcactcggtg ttcgcgacgg cactcgcgtg cctcgaccgc agcattccgg
      541 cgttccacta catggtcgcg aaggccggcg gaaacgacat ccgctgcgcg ccgtacgcta
      601 catttggtac acaggaactc gccgacgaag cggtccgcgc cctcgatggc cggcaggcgt
      661 gcctcctctc gcatcatggg atgatcgcgg tcggagctga tctcgatgcg gcgctcgcga
      721 tggcggtcga ggtcgagtgg ctggccgaaa catactgccg cgtactccag attggcaatt
      781 cgtcgatcct cgatgccgac gagatcgctc gtaatcgaat gcagtttcta gactacggga
      841 cccaacgccg ctccaggtga cgtacggcgc aaatcgatgc ggtgcgaatc gcatcgattc
      901 gtggtgcggc cgcggcgcac gtcaaacctg gcgccgcgca cgctagcgac caggaacctg
      961 gcagcagcgc gtgtaagagt gaaagtccgt cccgttgagc gcgccggtcg agccgttgaa
     1021 ccagacgcaa cccaggaacc ctggctggga gagctcggtc ggtgcaacgc cgatgctctt
     1081 cgagatgagc gccaagccgg ctggggctac tggtccatac acgcacccga ggcccacggc
     1141 tgcgtacccc gttgggcacg cttgggccac cgcgaggtcc gtgtaattcg aaccggtggc
     1201 aacgttcccg gaccctgtcc cattgagtga tgcgaaggtg cagttgagcg ccgtcgcgcc
     1261 tggccgcgca aagactccaa agatgtcgac cacgagatcg gttggcgttc cgaagaactc
     1321 gatctgattg cttcccgtcg cggctcctga agtcgtaacg gcgccggcgt tcgaggcctg
     1381 cacggtcgga cccgactgat accagttgac catcgatgtc gagttcgccg agccggccgg
     1441 tcgcgcgccg agaaagccgg gctggaactg cggattgacg atgccgacgt tcatgttgac
     1501 ggcggcgata tcggaagcgt tgctgagccc ggccgcggtt acgatgttgc atccggccga
     1561 gccgccgtag ctcgcgcccg tctggccgag gtcgacaccg tagggcgtcg agccgaccat
     1621 cggcggcttc acgcgcgtat cgatgtagcg gcacggcgtg atcggaacat agacgaggtc
     1681 ggtcgtcgca ctgccgagcg ccttcgccac gcgtgggctg cgttgcatcg acgagaccgc
     1741 ggcggcggcc gaggcgaacc cgcccggaac gccgatcgcc tggacttcgg cgagcggcag
     1801 catgtagagc gcctcccgca accatcggag ttgctccgat gtgacggtcg agccatcatt
     1861 cgtttccgcg gctacgccga cgatctggtc gacgagacga agtttttccg cgaggcg
//
LOCUS       DASUCJ010000228         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1194621, whole genome shotgun sequence.
ACCESSION   DASUCJ010000228 DASUCJ010000000
VERSION     DASUCJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194621"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VFU90_02800"
     CDS             complement(<1..1295)
                     /locus_tag="VFU90_02800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU5478729.1"
                     /translation="MIAFPFTQTDWSALVPVAVVALTGLVVLLVDLLAPRTTNRYVPI
                     AIGVLGAVAAGVLAAQQFGWDYNAFFGGFMTGGFTTVFEEVVLIAAIGSLILYGAIGP
                     AERMAGTVALMLWSTCGAMLMAGAANLMMIFLGLELLSLALYCLCGLVDRKTARESAL
                     KYLILSSTASGFMLYGMALLFGATGSVMLADFVNPALAASALFWMGAGMFAIGLSFKL
                     SLVPFHAWAPDVYQGAPLPVTAFMSVATKAATLAVLARFIYAALPSAVSDRLLLPIWI
                     VAGISMILGNAGMLAQHDLKRLLAYSGIAQVGYILTALAGSTPLGLRYAIFYLTAYAF
                     MNLGAFAVAAALSDDGEQGSRLANYAGLGRRRPWLAAAMTFFLLALAGLPPTAGFLGK
                     ILILSSSVGAGYAWMAALLIAGTAISLYAYAKVIYAMYGP"
     gene            complement(1292..2680)
                     /locus_tag="VFU90_02805"
     CDS             complement(1292..2680)
                     /locus_tag="VFU90_02805"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5478730.1"
                     /translation="MLVLAAVVLPIVAGLLTFALPREDRGIARVAGTLVATATLAMIA
                     FSQGQEWSFRWLSRPFTAAFHFGATPVSFWLALLTALCTACAIAAVRLPRARAFVALM
                     LLLEGSMLGLFLARDLLLFALFWDLMLVPVFFGLGGWSARPATAWRYLIYNFSAGLLL
                     LLATAAYGVLTGSTDVIGETHAPLIGGWAPWIFGGFAVAFLVKTPVWPLHTWMPPTYA
                     DLPSPMVAVVSAVQSKAGLYGFIAIALTLMPHYVREYANVLIVLGAISLIYGAAAALV
                     QNDAKRIVAYSSLSHLGLIAIAIAVGNQLALQGAIVYLIAHGLFSAALFLLLGYVEER
                     EGTRSMMRLGGLGRSNPKLAGAFCIACLAALGLPGLAGFAGELVILIGVYQSGNLWPA
                     IVGLVAIVVASAYFLRLYQGIMNGPEVADIPVRHDLTWVEGLTVAPLLLALVLIGVQP
                     HAILAAFAAVTR"
     gene            complement(2680..4656)
                     /gene="nuoL"
                     /locus_tag="VFU90_02810"
     CDS             complement(2680..4656)
                     /gene="nuoL"
                     /locus_tag="VFU90_02810"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5478731.1"
                     /translation="MHHTLGVDGAYPMLVALWLLPLAGAILCWAFGPQLKVWAGRLAC
                     AAIGASFVLAALSWAAGTQNAGGALGAHQALFSWMAGFDFGLLLDPLSLVWTFVITGV
                     GFLIHFYSMGYMDGDRAYARFFAYMNFFVFAMLTLVLSDNFVGLLVGWGLVGLASYFL
                     IGFWFERRSAVAAARKAFVINVVGDVAIMFAIFAIVATVHSTGFADAFAAAGSYGSGL
                     LFAICIGLFVGAAAKSAQIPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLIARCAPL
                     WNASGDAQMLAGTIGALTAIAGAILGCAQWDIKRILAYSTMSQIGYMIMGVGVGAYQG
                     GVAHFFTHAFFKAQLFLAAGLIIHALADEQDVRRMGGLRKKMPFAFWAILTGVLSICG
                     IPFFSGFFSKDQVIYGALQHGHPWMYAAGIVTAGLTAYYMFRLLFVTFLGNYRGEVAP
                     AELGIRAVAPAPAAKHDAPGHAHAPAWVMAVPVGILIVPTVAIGAALMFGGENSPWAR
                     YFAPQFAARPLPAPPIGELATSAIVLLFVLAGIAVAWMRYATRSAQANAVERLRNESL
                     RMPAVLTNLFYFDAAIDLIFVRTSQLIGTLFARAIDPHVIDGAVRELAEVTQTVGTFM
                     RSIETGLVRAYALILAFGAACFIVYYALMAGGPH"
     gene            complement(4662..4967)
                     /gene="nuoK"
                     /locus_tag="VFU90_02815"
     CDS             complement(4662..4967)
                     /gene="nuoK"
                     /locus_tag="VFU90_02815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5478732.1"
                     /translation="MTVALGNYIALSAVMFFIGVAGVLLRRNPLVILMSIELMFNAAN
                     LLFMAFARAWFANAGQIFAFLVITVAAAEAAIGLAIVVTTFRDEKNVDVDEIRLMRG"
     gene            complement(4967..>5543)
                     /locus_tag="VFU90_02820"
     CDS             complement(4967..>5543)
                     /locus_tag="VFU90_02820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5478733.1"
                     /translation="AIVTISAVWTVVAKKPVYSVVALLANFAALAVLYVTLSAEFLAV
                     MQIIVYSGAILVLFVFVIALLSSGVAPFSIGPNRMPKAAVPAAVVVLAALGFLVYATL
                     GAGEPRSGVHDTSTIGPVGTAGVFGSVADFGKALFTTHLLPFEATALILMVAVIGVVL
                     LAGDRLPHAPSRRRAEQVEREMREAILRSGE"
BASE COUNT         1147 a         1944 c         1750 g          702 t
ORIGIN      
        1 gggccgtaca tggcgtaaat caccttcgcg tacgcgtaga gcgaaatcgc ggtgcccgcg
       61 atcagcagcg ccgccatcca cgcgtatccc gcgccgacgc tcgacgagag gatcagaatc
      121 tttccgagga aaccggcggt gggcggcaac ccggccagcg cgagcaggaa gaacgtcatc
      181 gccgccgcga gccacggacg ccgccgcccg agccccgcgt aattcgcgag ccgagatccc
      241 tgttcgccgt cgtccgaaag tgccgccgcc accgcgaacg cacccaggtt catgaaggcg
      301 tacgcggtga gatagaaaat tgcgtagcgc agcccgagcg gcgtacttcc ggctagcgcg
      361 gtgaggatgt agccgacctg cgcgatcccg gagtaggcca gcagacgctt gagatcgtgc
      421 tgcgcgagca tcccggcgtt tccgaggatc atcgaaatgc cagcgacgat ccaaatcggc
      481 agcagcaggc ggtccgagac ggccgacggc agcgccgcgt agatgaagcg cgccagcacc
      541 gccagcgtcg ccgctttggt cgcaacgctc atgaacgcgg ttaccggcaa cggcgcgccc
      601 tggtagacgt cgggcgccca cgcatgaaac ggcacgaggc tcaacttgaa cgacaaaccg
      661 atcgcaaaca tcccggcgcc catccagaag agcgcgctcg ccgcgagcgc cggattgacg
      721 aaatcggcga gcatcacgct gccggtggcg ccgaagagca gcgccattcc gtagagcatg
      781 aagccggatg cggtcgaact gaggatcaag tacttcaatg cggattcgcg cgccgtcttg
      841 cgatcgacca gcccgcacaa acagtagagt gcgagcgaca gcagctcgag cccgaggaaa
      901 atcatcatta agttcgccgc gccggccatc agcatcgcgc cgcacgtgct ccagagcatc
      961 agcgccaccg tgccggccat ccgctcggcc gggccgatgg cgccgtagag aatcagcgaa
     1021 ccgatcgccg cgatcagcac gacctcttcg aacaccgtcg tgaaaccgcc ggtcatgaag
     1081 ccgccgaaaa aggcgttgta gtcccacccg aactgctgcg cggcgagcac gccggcggcg
     1141 accgcgccga gtacgccgat cgcgatcggc acgtagcggt tggtggttcg cggtgcgagc
     1201 aaatcgacga gcagcacgac cagtccggtc aaggcgacca ccgcaaccgg caccagcgcc
     1261 gaccagtcgg tttgcgtgaa cggaaacgcg atcatcgcgt taccgccgca aacgccgcta
     1321 agatcgcgtg cggctgcacg ccgatcagca ccagtgcgag cagcagcggc gcgaccgtca
     1381 gcccctcgac ccacgtgaga tcgtgccgca ccggaatatc cgccacttcc ggcccgttca
     1441 tgatgccttg gtacaagcgc aggaagtatg ccgaggcgac cacgatcgcg acgagcccca
     1501 cgatcgccgg ccacaggttc cccgactgat agacgccgat caggatgacc agctcgccgg
     1561 cgaacccagc cagcccgggc aggccgagcg ccgccaggca cgcgatgcag aacgcgcccg
     1621 cgagcttcgg gttcgaccgc ccgagcccgc ccagccgcat catcgagcgc gtcccctcgc
     1681 gctcctcaac gtatccgagc agcaggaaca gcgccgccga gaacagcccg tgcgcgatga
     1741 ggtacacgat cgcaccctgc agcgcgagct gattcccgac cgcgatcgcg atcgcgatca
     1801 agccgagatg cgagagcgac gagtacgcga cgatccgttt ggcgtcgttt tggacgagcg
     1861 ccgcggccgc accgtagatc aacgatatgg cgcccagtac gatcaaaacg ttcgcgtact
     1921 cgcgcacgta gtgcggcatc agggtgagcg cgatcgcgat gaagccgtac agaccggctt
     1981 tggattgcac cgccgagacg accgccacca tcggcgacgg cagatccgca tacgtcggcg
     2041 gcatccacgt gtgcagcggc cagaccggcg tcttcaccaa gaacgcgacc gcgaagccgc
     2101 cgaaaatcca aggcgcccaa ccgccgatca gcggcgcgtg cgtttccccg atgacgtcgg
     2161 tcgatccggt gaggacgccg tacgccgcgg tcgccagcag cagcagcaag ccggccgaga
     2221 aattgtagat cagataacgc cacgcggtgg ccggacgcgc gctccagccg cccagtccga
     2281 agaagaccgg aacgagcatc aaatcccaga acagcgcgaa cagcagcagg tcgcgcgcca
     2341 ggaacaagcc gagcatagag ccttccagca gcagcatcag cgcgacgaac gcccgcgcgc
     2401 gcggcagccg gacggccgcg atcgcgcacg ccgtgcacag tgcggtcagc agcgcgagcc
     2461 agaacgacac cggcgtcgca ccgaagtgga acgccgccgt gaacgggcgc gacagccagc
     2521 gaaagctcca ttcttggcct tgcgaaaacg cgatcatcgc gagcgttgcg gtcgccacca
     2581 gcgttccggc gacgcgcgcg atcccgcggt cctcgcgcgg cagcgcgaac gtcagcagcc
     2641 ccgcaacgat tggaagtacc actgcggcga gcaccagcat cagtgcggtc ctcccgccat
     2701 cagcgcgtag taaacgatga agcacgccgc cccgaacgcc aggatcagcg cgtacgcgcg
     2761 caccagcccg gtttcgatcg agcgcatgaa ggtcccgacc gtctgcgtca cttcggccag
     2821 ctcgcgcacc gcgccgtcga tcacgtgcgg atcgatcgcg cgcgcaaaca gcgtgccgat
     2881 cagctgcgac gtgcgcacga agatcagatc gatcgccgca tcgaagtaga acaggttcgt
     2941 caggacggcg ggcatccgca gcgactcgtt gcgcagccgt tcgaccgcat tggcctgggc
     3001 gctgcgggtg gcgtaccgca tccacgcgac cgcgattccg gccagcacga acagcagcac
     3061 gatggccgac gtcgcgagct cgccgatcgg cggcgccggc agcggccgcg ccgcgaactg
     3121 cggggcgaag tagcgcgccc acggcgaatt ctccccgccg aacatcagcg ccgcgccgat
     3181 cgccaccgtc ggcacgatca agatgccgac cggtacggcc atgacccagg ccggcgcgtg
     3241 cgcgtgaccg ggcgcgtcgt gcttcgcggc cggagccggc gcaaccgcgc ggattccgag
     3301 ctccgccggc gcgacctcgc cgcgatagtt gcccaagaac gtgacgaaca gcaggcggaa
     3361 catgtagtac gcggtgagtc ccgcggtgac gatcccggcc gcgtacatcc acgggtgccc
     3421 gtgctgcagc gcgccgtaga tcacctgatc cttcgagaag aatccgctga agaacgggat
     3481 gccgcaaatc gacagcacgc cggtgagaat cgcccagaag gcaaacggca tcttcttccg
     3541 caggccgccc atccggcgca cgtcctgctc gtcggccagc gcgtgaatga tcagtccggc
     3601 ggcgaggaac aactgcgcct tgaaaaacgc gtgcgtgaag aagtgcgcga cgccgccttg
     3661 atacgctccg acgccgacgc ccatgatcat gtagccgatc tgcgacatcg tcgagtaggc
     3721 gaggatgcgt ttgatgtccc actgcgcgca gccgagaatg gcgcccgcga ttgcggtgag
     3781 cgcgccgatc gtgcccgcca gcatctgcgc gtcgccgctg gcgttccaca gcggcgcaca
     3841 gcgagcgatc agatagacgc cggccgtcac catggtggcg gcgtggatca gcgccgaaac
     3901 cggcgtcggg ccttccatcg cgtcgggaag ccacgtgtgc agcggaatct gcgccgactt
     3961 cgccgcggct ccgacgaaca gcccgatgca aatcgcgaac agcagccccg agccgtagct
     4021 gccggcggcc gcgaacgcgt cggcgaagcc ggtcgagtgc acggtcgcga cgatcgcgaa
     4081 gatcgcgaac atgatcgcga cgtcgccgac gacgttgatg acgaaggcct tgcgcgccgc
     4141 cgccaccgcc gagcgccgct cgaaccagaa cccgatcaga aaatacgatg cgaggccaac
     4201 cagcccccag ccgaccagca atccgacgaa gttgtcggag agcaccagcg tgagcatcgc
     4261 aaacacgaag aagttcatgt aggcgaagaa gcgcgcgtac gcgcgatcgc cgtccatgta
     4321 tcccatcgag tagaagtgga tcaagaaccc gacgccggtg atcacgaacg tccacaccag
     4381 cgagagcgga tccaacagca gcccgaagtc gaatcccgcc atccacgaaa acagcgcctg
     4441 atgcgccccc agcgcgccgc cggcgttttg cgttccggcc gcccacgaga gcgcggcgag
     4501 cacgaacgag gcgccgatcg ccgcgcaggc cagccgtccg gcccaaacct tgagctgcgg
     4561 tccgaacgcc cagcacagga tcgcgcccgc gagcggcagc agccacagcg cgaccagcat
     4621 cggataggcg ccgtcgacgc cgagggtgtg atgcatcggg ctcatccgcg catcaaccgg
     4681 atttcgtcga cgtccacgtt cttctcgtcg cggaacgtcg tcaccacgat cgccaaaccg
     4741 atcgccgcct ccgccgccgc caccgtgatc accaagaacg caaagatttg gccggcgttc
     4801 gcaaaccacg cccgcgcgaa cgccatgaac aacaggttgg cggcgttgaa catcagctcg
     4861 atgctcatca gaatgaccag cgggttgcgg cgcagcagca cgcctgccac gccgatgaag
     4921 aacatcacgg ccgaaagtgc gatgtagttc ccgagcgcga ccgtcactat tctccgctcc
     4981 gcagaatcgc ttcgcgcatc tcgcgctcga cttgctcggc gcgccggcgc gacggcgcgt
     5041 ggggcagacg gtcgccggcc agcagcacga ccccgatcac ggccaccatc aaaatcagtg
     5101 cggttgcttc gaacggcagc aagtgcgtcg tgaacagcgc cttcccgaag tcggccacgc
     5161 tgccgaacac gccggccgtg ccgaccggac ctatagtcga cgtatcgtgc acgccgctgc
     5221 gcggttcgcc ggctcccagc gtcgcatata ccaggaagcc cagcgccgcc agcacgacga
     5281 ccgccgccgg gacggcggcc ttcggcatgc ggttgggccc gatcgagaac ggcgcaacgc
     5341 cactgctgag cagcgcgatc acgaagacga acagcacgag gatggcgccc gagtacacga
     5401 tgatctgcat caccgcgaga aattcggccg acagcgtgac gtacagcacg gccagcgccg
     5461 cgaagttcgc cagcagcgcg accacgctgt acaccggctt cttggcgacc accgtccaca
     5521 cggcgctgat cgtgacgatc gcg
//
LOCUS       DASUCJ010000229         5651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1439009, whole genome shotgun sequence.
ACCESSION   DASUCJ010000229 DASUCJ010000000
VERSION     DASUCJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439009"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..523
                     /locus_tag="VFU90_02825"
     CDS             92..523
                     /locus_tag="VFU90_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEU5478734.1"
                     /translation="MRALFDAGVAAVDLAGAGGTSWSEVERHRIVEPWRARVAGAFAG
                     WGIPTAECLRTARTIAPTETLIASGGIRTGLEALKGLALGADAVGIAGPFLRAASAGY
                     AEAAEFARELIEVLRVGMFAVGRRTLGELRSFPCLTRVAPG"
     gene            565..1404
                     /locus_tag="VFU90_02830"
     CDS             565..1404
                     /locus_tag="VFU90_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU5478735.1"
                     /translation="MPNPQTSQPPLDLAAADAYCRYLTRRHYENFSVASRFVEPERRL
                     DLARIYAYCRTTDDLGDESPDGTALPRLERWRDEVGALFAGVPPVHPVLRALQVTIAR
                     RNIPATPFLDLIEANVLDQRVKTYATWAELQRYCTLSAAPVGRMVLRVFGMTDPEEER
                     LSDDVCIGLQLANHAQDVSRDAAIGRTYLVQEDIDRGGRPEAARALVARARALLDSGR
                     ALEARAPRALRLQLSLYRLGGLAICDAIERIGYDTGKKRPSVSSGTKVSLVLRAALEA
                     VTP"
     gene            1401..2294
                     /locus_tag="VFU90_02835"
     CDS             1401..2294
                     /locus_tag="VFU90_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU5478736.1"
                     /translation="MINLQRALRGMAPPETDQHRAERFNRDMARREAGNFYWGFISLG
                     HHERMAIYALYNFARQVDDEADSIGLENLPERLQAHRERISMAVRGDYGEDPILRVLA
                     EAIDRYGIPERELQMLIDGVAMDFGNSRYATFDDLRTYCNLVASVVGRMCVRIFGFSD
                     PVALERADDLGLALQLTNILRDVREDAVDMHRIYLPQEDLARFAIPESSLYSGELAAG
                     WTDLVAYQVERARRYFATGYEVLQYIPRRPAACVHTMAGIYEELLKKIERDPGLPLRE
                     RAALSKTEKLRVVVRSWLSSA"
     gene            2276..3586
                     /gene="hpnE"
                     /locus_tag="VFU90_02840"
     CDS             2276..3586
                     /gene="hpnE"
                     /locus_tag="VFU90_02840"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEU5478737.1"
                     /translation="MAQQRIAVVGAGLAGLSAGLDLKDAGTRVELFERSRLLGGRATS
                     FEVDGVEVDNGQHVFLACCTEFVAFAKRVGMERFLHLQDRFDALVLARDGRAGRLRAG
                     SLPAPLHLAGSFLRYPHLSGGAKLRVVRALAAALASGAGAGSFEAWLERTGQNAETRR
                     AFWDPFFIPALNAPFERVDNADAMFVLSTAFLKNATAARFGFSTVPLAHFAAAAAARF
                     DAVHLSTAVLGIEPSEANVALRLLDGRSVAFDAVVLAVPPRALAKLLGDPEAYGIRDL
                     DAFDPFPIVDVHLWHDGGRAGFDFAAALDSPLQWIFEKTPGYLCCSFSAADRYARLGT
                     AELESFAWSEVREFVPALRAAALLRSSVTRNPEATYLPRLGVRRPAQRTTHPAVAVAG
                     AWTETGWPDTMESAVRSGRAAARALVSEVRAVERHDPYRSGIAS"
     gene            3586..5487
                     /gene="shc"
                     /locus_tag="VFU90_02845"
     CDS             3586..5487
                     /gene="shc"
                     /locus_tag="VFU90_02845"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU5478738.1"
                     /translation="METSQALNASVEWLLAKQSPEGWWSGELETNVTMTAEQVLLYRF
                     LGLPIDGVRERAIAHILHHQRSDGSWALYFDGPADVSTTIEAYVALKVLGVDPARDEM
                     RRALAVIHRAGGVAEARVFTKIWLALFGKYPWDGVPSLPPEIAYFPPWMPFNLYDFAC
                     WARGTVAPLTIVVSRKPVRDLGVDVSEIVRPGSEAELHRVRGSGWLMTVEKLQKLYER
                     LPFQPFRKAAQRRVAEWIVERQEADGSWGGIQPPWVYSLIALDLMGYSLDHPVMRKGI
                     AGFARFTIDDDQGWRFLACMSPVWDTAWAVRALALAGFDRSHPAMQRAVAWLLREQIP
                     DDAPGDWRVKCREKRGNRWAFEFDNDAYPDIDDTTIVVLALLEGGDPAKVASSVERAR
                     VWTLAMGSKNGAWAAFDRDNTRELLYRMPFSDFGAMIDPPTEDVTAHVLEMLAALGAD
                     STDPAVARGLAYLRETQRPGGYWYGRWGVNYVYGTWCVISALGALKTGRDMIERGAAW
                     LLKVQNPDGGWGESCHSYVDESFAGVGASTASQTAWAVNALQIAGLAQHPATQRGLGY
                     LCESQLPDGTWDEPECTGTGFPRDFYINYHLYRHLFPVMALAVDVTQHPPPASAQANE
                     GLLASERVT"
BASE COUNT          922 a         1776 c         1944 g         1009 t
ORIGIN      
        1 cggactcctt gcgaagattg cggagctttg ccgatgcgtc gactttcctc tcgtcgtata
       61 ggaggtcggc tgggggatcg cgcccgagtg cgtgcgagcg ctcttcgatg cgggcgtagc
      121 cgccgtcgat cttgccggcg cgggcggtac gtcctggagc gaggtcgagc ggcaccgcat
      181 cgtagagccg tggcgcgcgc gcgtcgccgg cgcatttgcc ggctggggta ttccgacggc
      241 cgagtgcctg cggacggcgc ggacgatcgc gccgaccgaa acgctgatcg cgagcggagg
      301 gatacgcacc ggcctggagg cgctcaaagg gctcgcatta ggcgccgacg ccgtcggaat
      361 cgccggcccg ttcttgcgtg cggcgtcggc aggttacgcc gaagccgccg agtttgcgcg
      421 cgagctgatc gaggtcttgc gcgtcggaat gttcgcggtc gggcgacgca cgctcggcga
      481 gctgcgctcg tttccctgtc tgacgcgcgt cgcaccgggg tgagcaaggg cggttacgcg
      541 gctgcgggaa agattcggcc gtcgatgcca aacccgcaaa cctcgcagcc cccgctcgat
      601 ttagctgccg ccgatgccta ctgccggtat ttgacccgcc ggcattacga gaacttcagc
      661 gtcgcttcgc gattcgtcga gccggaacgg cgcctcgatt tggcgcgcat ctacgcgtat
      721 tgccgcacca ccgacgatct cggcgacgaa agcccggacg gcacggcgtt gccgcgcttg
      781 gaacgctggc gcgacgaggt cggcgcgctc ttcgcgggcg tgcctccggt tcatccggta
      841 ttaagagcgt tgcaggtgac gatcgcacgc cggaacattc cggcgacgcc ctttctggat
      901 ctgatcgaag cgaacgtcct cgatcagcgc gtaaaaactt acgccacgtg ggcggagctc
      961 caacggtact gcacgttgtc ggccgcaccg gtcggccgca tggtgttgcg cgttttcggg
     1021 atgacggatc cggaagagga gcggctctcc gacgacgtat gtatcgggct ccagctcgcc
     1081 aatcacgcgc aagacgtgag ccgcgacgca gccatcgggc gcacctatct cgtgcaggaa
     1141 gacatcgatc gaggcgggcg gcccgaggcg gcgcgcgcgc tagtcgcccg tgctcgcgcg
     1201 ctgctggatt cggggcgcgc cctggaagca cgggcgccgc gagcgctgcg gctgcagctg
     1261 tcgctctatc ggctgggcgg gctcgcgatc tgcgacgcga tcgagagaat cgggtacgat
     1321 accggcaaaa agcggcccag cgtctcgagc gggacgaagg tatctctcgt actgcgcgcc
     1381 gcattagagg cggttacgcc gtgatcaacc tgcagagagc cttacgcggg atggccccgc
     1441 cggagaccga ccagcaccgt gccgaacgct tcaaccgcga catggcgcgg cgcgaagccg
     1501 gaaatttcta ttgggggttt atctccctcg ggcatcacga gcggatggca atttacgcgc
     1561 tgtataattt tgcgcgccaa gtcgacgacg aagccgacag catcgggctc gagaacctgc
     1621 cggaacgatt gcaggcgcac cgcgaacgga tttccatggc ggtgcgcggc gattacggcg
     1681 aggatccgat cttgcgcgtg ctcgccgagg cgatcgatcg ctacggaata cccgagcgcg
     1741 aactgcagat gctcatcgac ggagtcgcga tggacttcgg caattcgcgg tacgcgacgt
     1801 tcgacgacct gcggacgtat tgcaacttgg tcgcctcggt cgttgggaga atgtgcgtgc
     1861 gcatcttcgg gttcagcgat ccggttgctc tggagcgggc cgacgacttg ggcttggcgc
     1921 tgcagctgac gaacatctta cgcgacgtac gcgaagatgc ggtcgacatg caccgcatct
     1981 acctgccgca ggaagacctc gcacggttcg cgattcccga atcgtcgctg tacagcggcg
     2041 agctcgccgc cggctggacc gatttggtgg cgtatcaagt cgaacgcgcg cgccgctatt
     2101 tcgcaaccgg ctacgaagtg ctgcagtaca ttccgcgccg gcccgcagct tgcgtgcata
     2161 cgatggcggg catctacgag gagctgctca agaaaatcga gcgcgatccg ggtttgccgc
     2221 tgcgcgagcg ggcggcgctt tcgaagaccg aaaagctgcg cgtcgtggtg aggtcgtggc
     2281 tcagcagcgc atagcggtcg tcggcgcggg gctggccggc ctgtccgccg gcctcgatct
     2341 caaggacgcg ggtacgcgcg tggagctctt cgaacgcagc cggctgctcg gcgggcgggc
     2401 gacgtcgttc gaagtcgacg gagtcgaagt agacaacggc caacacgttt tcttggcctg
     2461 ctgtaccgag ttcgtcgcgt ttgccaagcg ggtcgggatg gagcggttcc tgcacctgca
     2521 ggatcgtttc gacgcactgg tgctcgcgcg cgacggccgc gcgggccggt tgcgcgcggg
     2581 cagtcttccc gcgccgctgc acctcgctgg ctcctttctg cgctatccgc atttgagcgg
     2641 cggcgcgaag ctgcgggtcg tacgcgcgct ggcggctgcg ctcgcttcgg gcgcgggcgc
     2701 cggaagcttc gaagcgtggc tggaacgaac ggggcagaac gcggagacgc gccgggcgtt
     2761 ttgggatccg ttctttatcc cggcgctgaa cgcaccgttc gagcgcgtcg ataatgccga
     2821 cgcgatgttc gtcttgtcga cggcgttttt aaagaacgcg acggccgcac ggttcggctt
     2881 ttcgacggtg ccgctcgcgc acttcgcggc cgccgccgcc gcgcggttcg acgcggtgca
     2941 tctttcgacg gccgtcctcg gaatcgagcc gagcgaagcc aacgtggcac tacggctgct
     3001 cgacggccgc agcgtcgcgt tcgacgccgt cgtgctcgcg gtgccgcctc gagcgctcgc
     3061 caagctcctc ggagatccgg aggcgtacgg aattcgcgat ctcgatgcgt tcgatccgtt
     3121 tccgatcgtg gacgtgcact tgtggcacga cggcggacgt gccgggttcg acttcgcggc
     3181 agcgctcgac tcgccgctgc agtggatttt cgaaaagacg cccggctatt tgtgctgcag
     3241 cttcagcgcc gccgatcgct acgcgcgcct ggggaccgcc gagctcgaga gtttcgcctg
     3301 gagcgaagtg cgggagttcg tgccggcgct gcgcgccgcc gcgctgcttc gcagctcggt
     3361 gactcgcaac ccggaagcga cctatctgcc gcgtctcggc gttcggcgtc cggcgcagcg
     3421 gacgacgcac ccggcggtcg cggtcgcggg ggcttggacg gagacgggct ggcccgacac
     3481 gatggaatcg gccgtccgca gcggacgcgc cgcggcccgg gcgcttgtga gcgaagtgcg
     3541 tgccgtcgaa cggcacgacc cgtatcgcag cggtatcgcg tcgtgatgga aacgtcacaa
     3601 gcgctgaacg cgagcgtcga atggctgctt gcgaagcagt cgcccgaagg ctggtggtcg
     3661 ggggaactcg agaccaacgt aacgatgacc gccgagcagg tgctcttgta tcgttttctc
     3721 gggctgccga tcgacggcgt gcgcgagcgt gcgatcgcgc acattctgca ccatcaacgc
     3781 agcgacggct cgtgggcact ctatttcgac ggccctgccg acgtcagcac gacgatcgaa
     3841 gcctacgtcg cgctcaaagt tctcggcgtg gaccccgccc gcgacgaaat gcgccgtgca
     3901 ctggccgtga tccaccgagc cggcggcgtc gccgaggcgc gggtgtttac gaaaatctgg
     3961 ctcgctttgt tcggcaagta tccctgggac ggcgtcccgt cgttgccgcc cgaaatcgcg
     4021 tacttcccgc catggatgcc gttcaatctc tacgatttcg cgtgttgggc tcgcggcacg
     4081 gtcgcaccgc tgacgatcgt cgtctcgcgg aagcccgttc gagatttggg agtcgacgtc
     4141 tcagaaatcg tccggccggg cagcgaagcc gagctgcacc gggttcgcgg ttcgggatgg
     4201 ctcatgacgg tcgagaagct tcaaaagctc tacgaaaggt tgccgtttca accgtttcgc
     4261 aaagccgcgc agcgccgcgt cgccgagtgg atcgtcgagc ggcaagaagc cgatggaagc
     4321 tggggcggga tccagccgcc gtgggtatac tcgctgatcg cactcgatct gatgggttac
     4381 tcgctcgatc atccggtgat gcgcaagggg atcgccggtt tcgcgcggtt tacgatcgac
     4441 gacgatcaag gctggcgttt tctggcgtgc atgtcgccgg tgtgggacac ggcctgggcc
     4501 gtgcgagcgc tggcgctggc cggattcgac cgttcgcacc ccgccatgca gcgcgccgta
     4561 gcctggctgt tgcgcgaaca gattccggac gacgcgcccg gcgactggcg ggtgaagtgc
     4621 cgcgaaaagc gcggcaaccg ctgggcgttc gagttcgaca acgacgcgta tccggacatc
     4681 gacgatacga cgatcgtggt gctcgcgctg ctggaaggcg gcgatcccgc gaaggttgca
     4741 tcgtcggtcg agcgcgctcg cgtttggacg ctggcgatgg gctcgaagaa cggcgcgtgg
     4801 gcggccttcg atcgcgacaa cacccgcgaa ctgctctacc gcatgccgtt ttctgatttc
     4861 ggagcgatga tcgatccccc gaccgaagac gtcaccgccc acgttctcga aatgttggcc
     4921 gcgctcggcg cggattcgac cgatccggcc gtcgctcgcg ggctggccta cttacgcgaa
     4981 acgcagcgcc ccgggggtta ctggtacggg cggtggggag tcaactacgt ttacggaacg
     5041 tggtgcgtca tctcggcgct cggcgcgctg aaaaccggac gcgatatgat cgagcgaggc
     5101 gcggcctggc tgttgaaagt gcaaaacccg gacggcggct ggggcgagag ctgtcattcc
     5161 tacgtcgacg agtcgtttgc cggtgtggga gccagcacgg cttcgcagac ggcttgggcg
     5221 gtgaacgctc tgcaaattgc cggcctcgcg caacacccgg cgacgcaacg gggtcttggc
     5281 tacttgtgcg agagccagct gcccgacggc acgtgggacg agcccgaatg caccggaacc
     5341 ggcttccctc gcgactttta cattaactat cacctttatc gccatctctt cccggtgatg
     5401 gcgctcgcgg tcgacgtcac gcagcacccg ccgcccgctt cggcccaggc aaatgaaggc
     5461 ctcctcgcct cggaaagggt aacctgaacg catgagcatg cccctgcagc agaagatcgc
     5521 cgtcgcgaag tacatcgcgg gcaagacgct caaaggcgaa aaaaagtatc cgctcgtgct
     5581 agagctcgag ccgctgttgc aatgcaatct ggcgtgcgcc ggctgcggga agatccagca
     5641 tcctgacgac a
//
LOCUS       DASUCJ010000230         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_598312, whole genome shotgun sequence.
ACCESSION   DASUCJ010000230 DASUCJ010000000
VERSION     DASUCJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598312"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..851
                     /locus_tag="VFU90_02850"
     CDS             96..851
                     /locus_tag="VFU90_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478739.1"
                     /translation="MMMRTLRLAPFVAAFVACAAASLGAQQIPGTAAKNAARRVVAAT
                     NAHTAAMQNTDGPAATPTRTQAPATPTRVAESNRSARMPEQPKAATPAAATTPAAHGR
                     APADRAARSAASKGKDTLTVTAQGPNGDGTTSISERGGKNEIGLMRESFSYNADGRRD
                     PFVSLIESGDLRPMVSDLRLVVVIYDPAGRSVAILRDVSTKEQYRVKTGQTLGRMRVA
                     RIDLKSVTFTLEEFGYSRQETLALNDSTRARSQ"
     gene            848..>1506
                     /locus_tag="VFU90_02855"
     CDS             848..>1506
                     /locus_tag="VFU90_02855"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEU5478740.1"
                     /translation="MRRVCALIGMTFAALAAQASVARAATVGKLGGGEVTSLSVVPTA
                     GRAEVVVAVDGSVDVDDFSLASPPRIVLDLRGAKLVAGGRMYDRIARGGITNVRVAQY
                     RDNIVRLVIDLDGQHSYTVNRTLNEVHVVLDPNGTTKTSFTAWRASGDNADAFDAAAD
                     ATPSYRAAGTARHSSVKLTSPDATAAISSQPKITVTYQDTDIKDVIAGFAVFSGRTIV
                     AG"
BASE COUNT          275 a          528 c          482 g          221 t
ORIGIN      
        1 gtgatcgagg cgcacttcca actgcagacg ttcgtgacgc acaaccccgc cgaagatgcg
       61 gacgatcagc ctcccgcgaa gaagggggcg aaatcatgat gatgcgtact ctcaggctcg
      121 cgccgtttgt cgcggccttc gtcgcatgcg ctgccgcatc gttaggcgcg cagcagatcc
      181 cgggaacggc cgcgaagaac gcggcgcgcc gggtggtggc agcgacgaac gcgcacaccg
      241 ccgcgatgca gaacaccgac ggcccggcag caacgccgac gcgcacgcaa gccccggcga
      301 cgccgacacg tgtcgccgag tcgaatcgca gcgcgcggat gccggagcag ccgaaggcgg
      361 cgacgccggc cgctgccacg acaccggcgg cgcacggccg tgcaccggct gatcgtgccg
      421 cacgctctgc agcatcgaag ggcaaggaca cgctcacggt caccgcgcag ggtccaaacg
      481 gtgacggcac gacatcgatc tccgagcgcg gcggaaagaa cgaaatcggc ctcatgcgcg
      541 agtcgttctc ctacaacgcg gatggacgcc gcgatccgtt cgtgtcgttg atcgagtccg
      601 gcgacctccg accgatggtc tccgatctcc ggctcgtggt cgtcatctac gatcccgctg
      661 gccgatccgt cgccattctg cgcgacgtgt cgacgaagga gcagtaccgc gtcaaaacgg
      721 ggcagacgtt aggccgcatg cgcgtcgcgc gcatcgacct caagtccgtc acgttcaccc
      781 tggaagaatt cggatacagc cggcaagaaa cgctggcgct caacgattcc acgagagcga
      841 ggtcgcaatg aggcgggtct gtgcgctgat cggaatgacg ttcgccgcgc tcgccgcgca
      901 ggcgtccgtc gcccgcgccg cgacggttgg caagttaggc ggcggtgagg tgacgtccct
      961 cagcgtcgtg cccacggccg ggcgcgctga ggtcgtcgtc gctgtcgacg gctcggtcga
     1021 cgtcgatgat ttctcgctgg ccagtccgcc tcgcatcgtc ctcgacctgc gcggcgccaa
     1081 gctcgtcgcg ggcggtcgca tgtacgatcg catcgcgcgc ggcggcatca ccaacgtgcg
     1141 cgtcgcccaa taccgcgaca acatcgtccg tctggtgatc gacctcgacg gccaacacag
     1201 ctacaccgtc aatcgcacac tgaacgaagt ccacgtcgtg ctcgatccga acggcacgac
     1261 gaaaacgtcg ttcaccgcgt ggcgcgcgtc cggcgacaac gcagacgcct tcgatgccgc
     1321 cgcggacgcc acgccgtcat atcgcgccgc gggtacggcc cgtcacagct ccgtgaagct
     1381 cacatcgccc gatgccacgg ccgcgatatc gtcgcagccc aagatcaccg tcacctacca
     1441 ggacaccgac atcaaggatg tgatcgcggg gttcgccgtg ttctccggcc gcacgatcgt
     1501 cgccgg
//
LOCUS       DASUCJ010000231         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1117850, whole genome shotgun sequence.
ACCESSION   DASUCJ010000231 DASUCJ010000000
VERSION     DASUCJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117850"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFU90_02860"
     CDS             complement(<1..790)
                     /locus_tag="VFU90_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478741.1"
                     /translation="MLGTLSVTLGAVIAVFAIARAVLIRPLPYPDPERLVWLSAASDS
                     RPAGLFSLPEFLELTSRVRSADVEAYGTLSASLATAGPTIRVQGLRLSGGALSALGAR
                     PTRGRLLQAADDSIGAPRVVVLGFGFWRRQFGADPRVVGTTIRLNGERYSVVGILPVF
                     FPMPLRNIDLVVPLSPGLDPRRSLWGSSNFLRLLARERGSAGAGTVAAELNAIGTELR
                     QRLPDVYTSSLAIHLVGMKEFLVGDQRLGLFVLLGCVLLMQAIAF"
     gene            1065..2108
                     /locus_tag="VFU90_02865"
     CDS             1065..2108
                     /locus_tag="VFU90_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU5478742.1"
                     /translation="MDTLVLDRADVLQVLTPGECLDAVEAAFVAHAHGHTIPPGILGA
                     RATGGGFHIKSAGLLGRDEAARPMFAAKVNANFPGNPDRTGWPTIQGVIALFDASNGC
                     LLALLDSIEITSLRTAAATAVAAKYLAPETAIVTICGCGEQGRSQLRALASVRSITRV
                     MALDIDAGRAAGLATEMSGELGCDVDVVHAVSDAARDTTIWVTCTPALRWFLGRMHVA
                     PGAFVAAAGADNPDKQEIEPALLAANVVIADILEQCATIGDLHHAIDAGVMQRADVRG
                     ELAGVVSGRCIGRRSPDEIIIFDSTGTTLQDVAVAALVYERAVATGIGLRIDLGAANH
                     AETVNRHAARARS"
     gene            2105..>2849
                     /locus_tag="VFU90_02870"
     CDS             2105..>2849
                     /locus_tag="VFU90_02870"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEU5478743.1"
                     /translation="MSPASDAPAAAPWLLLIHQIPPKPDYLRVKIGRRLQRIGAVPVK
                     NSVYVLPDRAQSLEDFQWVRAEITDGGGDASICKAAFVDGLTNSQIEHLFRSARDADY
                     VEVAEAARELWGGIRRRDRDGQRAARPEDELSRLRKRIIAIAAIDFCNAPGRAMAEDA
                     VRTLATELEPAPTMATDTDASGSKPGVSAYRGRTWITRSGIFVDRIASAWLIRRFIDR
                     EARFKFVDSPKERPSRGELRFDMFEGEFTH"
BASE COUNT          520 a          932 c          946 g          451 t
ORIGIN      
        1 cgaacgcgat cgcttgcatg aggagtacac acccgagcag aacgaagagg ccgagccgtt
       61 gatcgccgac gagaaactcc ttcatcccga ccaggtggat cgcgagcgag gacgtgtaca
      121 cgtccggaag acgctgccgc agttcggtcc cgatcgcgtt gagctccgcg gcgacggttc
      181 ccgccccggc ggatccgcgt tcccgcgcca ggagtcggag aaaattggac gaaccccaca
      241 ggctgcgacg tggatcgaga ccgggcgaca gcgggacgac cagatcgatg ttgcgcagtg
      301 gcatggggaa gaagactgga agaattccca ccaccgaata ccgttcgccg tttaggcgaa
      361 ttgttgtccc gaccacgcgc ggatcggcgc caaactgccg ccgccaaaag ccgaacccga
      421 ggacaacgac ccgcggcgcg ccgatggaat cgtcggccgc ctggagcaaa cggccgcgcg
      481 tgggcctcgc gcccaatgcc gacaaggcgc cgccagacag gcggagcccc tgaacgcgaa
      541 tcgtcggtcc ggcggttgcc aacgacgcgc tcaacgtgcc gtaggcttcc acatccgccg
      601 atcgcacgcg gctggtcagc tccaagaact ccggcagaga gaacaatccg gcaggcctcg
      661 aatcgctggc cgcggacaac cacacgagac gctcgggatc gggatacggc agcggtcgga
      721 tcagcactgc gcgggcgatc gcgaacacgg cgatcacggc gcccagagtc acgctgagcg
      781 ttccgagcac caggattgtg aacgagggag ctcggcgaag cgcacgcgaa gcggtgcggc
      841 attcggacca ggcgcgttca gtggtaagca tctcgaaggg tgtgatcgcg atgattcatc
      901 tgaatcaacg ggtcatatta gcaactcaga tcgaggacgg caacaccagc gtgctcggct
      961 cgtcagagca gatcgcctcc agcacgacgt ggctcttgcg atgattcagt tgcgatagta
     1021 ctctgcgcca gacatggtgt cgccactcct ctggccagtg ggcgatggat actctcgttc
     1081 tcgatcgcgc cgacgtattg caggtcctga cgccgggcga gtgcctcgac gcggtcgaag
     1141 ccgcgttcgt cgcgcacgca cacggccaca ccatcccgcc cgggatcctc ggcgcgcgcg
     1201 cgactggtgg cggtttccac atcaagtcgg cgggactgct tggccgtgac gaggccgccc
     1261 ggccgatgtt cgccgcgaaa gtcaacgcca acttcccggg caatcccgat cggaccggct
     1321 ggccgacgat tcaaggcgtg atcgcgctgt ttgacgccag taacggttgc ctcctggcgc
     1381 tcctcgactc gatcgagatc acatcgcttc gcacggcagc cgcgaccgcg gtcgccgcga
     1441 aatacctcgc gccggagacg gcgatcgtca caatctgcgg ctgtggcgag cagggacgaa
     1501 gtcagctccg tgcgctcgcc agcgttcgct cgatcacgcg cgtcatggcg ctcgacatcg
     1561 acgccggccg cgccgcagga cttgccaccg agatgtcggg cgagctcgga tgcgacgtgg
     1621 acgttgtcca cgcggtgagc gacgccgccc gggacacgac gatctgggtg acgtgcacgc
     1681 cggccctccg ctggttcctc ggccgcatgc acgtcgcgcc gggggcgttt gtcgccgcgg
     1741 cgggcgccga taatccggac aagcaggaaa tcgaaccggc gctgcttgcc gcgaacgtcg
     1801 tgatcgcgga catcctcgag cagtgcgcga cgatcggcga tctgcatcat gcgatcgatg
     1861 cgggcgtgat gcagcgtgcc gacgttcgcg gcgaattagc tggtgtcgtg tcgggccgtt
     1921 gcatcggccg gcggtcgcca gacgagatca tcatcttcga tagcaccggc accacgttgc
     1981 aggatgtcgc tgtcgcggcg ctcgtttacg agcgcgcggt cgccacgggc atcggacttc
     2041 gcatcgatct cggcgcggcc aatcatgccg aaactgtgaa tcggcacgcg gcgcgggcac
     2101 gctcatgagc cccgcttccg acgcgcccgc tgctgcgccg tggctgttgc tcattcacca
     2161 gattccgccg aagccggact atctgcgcgt gaagattggc cggcggctcc agcgcatcgg
     2221 cgcggtgccc gtcaagaatt ccgtatatgt gcttcccgat cgcgcgcaat cgctcgagga
     2281 ctttcagtgg gtccgcgccg aaatcaccga cggcggtggc gacgcgtcga tctgcaaggc
     2341 ggccttcgtc gacgggctga cgaatagcca gatcgagcat ctcttccgga gcgcccgaga
     2401 cgcggactac gtcgaagtcg ccgaagcggc gcgtgagctc tggggaggca tccgccgacg
     2461 cgaccgcgac ggacagcgag ccgcgcgccc cgaggacgag ttgtcgcggc tacgaaaacg
     2521 catcatcgcg atcgccgcga tcgatttttg taacgcacct gggcgcgcga tggcggagga
     2581 tgcagtgcgg acgctggcga cggagctcga gccggctccc acgatggcga cggacaccga
     2641 cgcgagcgga tcgaaaccgg gagtttcggc ctatcgcggc cgcacgtgga tcacacgcag
     2701 cggcatcttc gtcgatcgca tcgccagtgc ctggctcatt cgccgcttca tcgatcgcga
     2761 ggcgcgcttc aagttcgtcg actctccgaa ggagcggccg tctcgcggcg agctgcggtt
     2821 cgatatgttc gagggcgaat tcacgcacg
//
LOCUS       DASUCJ010000232         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_124706, whole genome shotgun sequence.
ACCESSION   DASUCJ010000232 DASUCJ010000000
VERSION     DASUCJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124706"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VFU90_02875"
     CDS             <1..1331
                     /locus_tag="VFU90_02875"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5478744.1"
                     /translation="ALKALIAIPSVSADPAHEADVRRCCGRLVEAMRALGLEARVLET
                     GGNPLAYGEWTGAPGKPTVLVYGHYDVQPVDPVELWESPPFQGEVRDGKLFGRGAVDD
                     KGQVAMHLAAIEAHLRTRGRLPVNVKVVVEGEEEIGSPNFDAAVERYRHEFAADLAVI
                     SDTAVYAPDVPSLTTSLRGVVHLEIAIQGPEQDVHSGYFGGLLRNPIEALAGIIAGLK
                     DADGRVTIPGFYDGVEELDAASHARLRALPYDEAREARQLGVPELAGDRGRPPLERAW
                     FRPTLECNGIWGGYSGPGSKTIVPAWAKAKLSARLVGQQDPERVRGAIARYVERIAPA
                     GVRVTVVEEPGGVRPVTIAHDHPAVAAAARAMEAGFGKPPIFIGTGGSIGPVATFDRV
                     LHLPQVLIGVGLPDDCIHAPNEKFDLHQFFGGIKTIAAFYDELAAQEQTA"
BASE COUNT          230 a          429 c          476 g          207 t
ORIGIN      
        1 aggcgctgaa agcgctgatc gcgatcccgt cggtatcggc cgacccggcg cacgaagccg
       61 acgtccggcg atgctgcggc cgtttggtcg aagcgatgcg cgcgctcggc ctcgaagcac
      121 gcgtcctcga aaccggcgga aatccgctcg cctacggtga gtggacgggt gcgcccggaa
      181 aaccgacggt gctggtgtac ggccactacg acgtgcagcc ggtcgatccg gtcgaactgt
      241 gggagagccc gccgtttcag ggcgaggtgc gcgacggcaa gctgttcggc cgcggagcgg
      301 tcgacgacaa gggccaggtg gcgatgcatt tggcggcgat cgaagcgcat ctgcgcacgc
      361 gcggccgttt accggtgaac gtgaaagtcg tcgtcgaagg cgaggaagaa atcggctcgc
      421 cgaatttcga cgccgcggtg gaacggtatc gacacgagtt tgccgccgat ctcgcggtga
      481 tctccgacac ggccgtgtac gcgccggacg ttccttcgct cacgacctcg ctgcgcggcg
      541 tcgtgcacct ggagatcgcg atccagggac cggaacagga cgttcactcg ggttatttcg
      601 gcggtttgct gcgtaacccg atcgaagcgc tggccggcat catcgccggc ctcaaagacg
      661 ccgacggccg ggtgacgatt cccggttttt acgacggggt cgaagagctc gacgccgcgt
      721 cgcacgcgcg cttgcgcgcg ctgccgtacg acgaagcgcg cgaagcgcgg cagctcggcg
      781 tccccgagct tgccggcgac cgggggcggc cgccgctcga acgcgcctgg tttcgtccga
      841 ccctggagtg caacggaatc tggggcggat atagcggacc cggcagcaag acgatcgtgc
      901 cggcttgggc gaaagcgaaa ctctcggccc ggctggtcgg gcaacaggat ccggagcgcg
      961 tgcgcggcgc gatcgcgcgc tacgtcgagc ggatcgcacc cgccggcgta cgcgtgaccg
     1021 tcgtagagga accgggcggc gtgcgcccgg tcacgatcgc gcacgatcat ccggccgtag
     1081 cggccgccgc gcgcgcgatg gaagcgggct tcggcaagcc gccgattttc attggaacgg
     1141 gcggatccat cggtccggtg gcaaccttcg atcgcgtgct gcatctgccg caagtgctga
     1201 tcggcgtggg actcccggac gactgcattc acgcccccaa cgaaaagttc gacctgcatc
     1261 aattcttcgg cggcataaag acgatcgcgg ccttttacga cgagctggcc gcgcaagagc
     1321 aaacggcgtg agcgacgagc gc
//
LOCUS       DASUCJ010000233         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_185638, whole genome shotgun sequence.
ACCESSION   DASUCJ010000233 DASUCJ010000000
VERSION     DASUCJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185638"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFU90_02880"
     CDS             <1..897
                     /locus_tag="VFU90_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478745.1"
                     /translation="DLVSRKGGIVAAATRAWIAGETGLIVHNYGGIGGKRREKAASGP
                     FYFGHFAYGLARAVECPLSGELRLEITYYQVYTQNGDGLIAGSLDWSRYMADRQYGWA
                     GVRPVCEAVLRHAAFTTAFDLNGESSASALDALVAQLEVMTARYRIGDGTGGTFVGFS
                     NNCSQDSNRALFATLRALRRYVEAHPHLEEWIARSPQEYARYREFLALTEDLRKKLQP
                     FGAPRRDWSDNEYNLGTTIEDAPMANVIAALGSWRCILPRLAFNTIAGTFFRHGASGS
                     VLGTNQIGGERPDVEPVVPLAL"
     gene            949..1290
                     /locus_tag="VFU90_02885"
     CDS             949..1290
                     /locus_tag="VFU90_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478746.1"
                     /translation="MEEVRDGVTDADATGMIADEQRITEAETLLPLLSERPTLPADDL
                     HAALPPDHDAHAAIDRLHAEIQSAAPSRTAIEEHVHRLRALPELEASVALWWESPGTQ
                     RFLWNLSQIGL"
     gene            complement(1326..>1510)
                     /locus_tag="VFU90_02890"
     CDS             complement(1326..>1510)
                     /locus_tag="VFU90_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478747.1"
                     /translation="ALHDWSKRHGVTAFVPPPHYCTDNAAMIAASADRQGNAVRVDAL
                     TLSADPNLAFELPPAA"
BASE COUNT          266 a          472 c          520 g          252 t
ORIGIN      
        1 gatttggtat cgcgcaaagg cgggatcgtg gcggccgcga cgagagcgtg gattgccggc
       61 gagacgggat tgatcgtgca caactacggc gggatcggcg ggaagcgccg cgagaaggcc
      121 gcgtcgggtc cgttctattt cggccatttt gcgtacgggc tcgctcgggc cgtcgagtgt
      181 ccgctatcgg gcgagctgcg tctcgaaatc acgtactatc aggtgtacac gcagaacggc
      241 gacgggttga tcgccggctc gctcgactgg tcgcgctaca tggccgatcg tcagtacgga
      301 tgggccggcg tgcgaccggt gtgcgaggcc gtgcttcggc acgcggcctt tacgaccgcg
      361 ttcgatctca acggcgaatc gagcgcgtcg gcgctcgacg cactggttgc ccagctggag
      421 gtgatgacgg cgcgttaccg gatcggcgac ggcaccggcg ggaccttcgt gggtttttcg
      481 aacaactgct cgcaagattc caaccgcgcg ttgttcgcaa ccttgcgggc gttgcgccgg
      541 tacgtcgaag cccatccgca cctcgaggaa tggatcgcgc gctctccgca agagtacgcg
      601 cgataccggg agttcctcgc gctgaccgaa gacctgcgca agaagctgca gcccttcggc
      661 gctccgcggc gcgactggag cgacaacgag tacaatctcg gtacgacgat cgaagacgcc
      721 ccgatggcga acgtgattgc agcgttgggg agctggcgct gcattttacc gcggctggcc
      781 ttcaacacga tcgccggcac gttctttcgg cacggcgcat ccgggagcgt tctcgggacg
      841 aatcagatcg gcggcgagcg ccccgacgtg gaaccggtgg tgccgctcgc gctttagggt
      901 ttcgcgctgc aaaggacaag cggaagaaac ctggtacacc tgcggcctat ggaagaggta
      961 cgagatggcg tcaccgacgc cgacgcgacc gggatgatcg cggacgagca gcgcatcacc
     1021 gaagcggaaa cgctgcttcc gctgctgtcg gagcggccca cgctgccggc cgacgatctg
     1081 cacgccgcgc tgccgcccga tcacgacgcg cacgccgcaa tcgatcggct gcacgcggaa
     1141 attcaaagcg ccgctcccag ccgcacggcg atcgaggaac acgtccatcg tttgcgcgcg
     1201 ctgcccgaac tggaagcctc ggtggcgctg tggtgggaga gtccgggaac gcagcggttt
     1261 ctttggaatt tgagccagat cggcttataa cggacgcccg accacgcacc gccgaccctc
     1321 cgaaattacg cggccggcgg aagctcgaac gcgagattcg gatcggccga aagcgtcaac
     1381 gcgtcgacgc gcaccgcgtt gccctgacgg tcggcggagg cggcgatcat cgcggcgttg
     1441 tcggtgcagt agtgcggcgg cggcacgaac gccgtcacgc cgtgccgttt gctccagtcg
     1501 tgtaaggcct
//
LOCUS       DASUCJ010000234         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_32975, whole genome shotgun sequence.
ACCESSION   DASUCJ010000234 DASUCJ010000000
VERSION     DASUCJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32975"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFU90_02895"
     CDS             complement(<1..195)
                     /locus_tag="VFU90_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5478748.1"
                     /translation="MKALVLSGGGAHGAYEAGVAQSLLQRERFDVICGVSIGAINAAL
                     IAAGKDAGSLERFWREELPSR"
     gene            complement(214..1908)
                     /locus_tag="VFU90_02900"
     CDS             complement(214..1908)
                     /locus_tag="VFU90_02900"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5478749.1"
                     /translation="MIGKRRLISGEMHYARVPRAYWSARLEMAYAMGLDAVSTYVFWN
                     LHEPRPEVYDFDGENDVAEYVRAAAAAGLDVILRPGPYVCAEWDLGGLPAWLLTDDSI
                     GLRTAGERFMRPVRRWLHRLGEELAQLQRSRGGPIVAVQLENEYGAFGEDPRYLQALR
                     EALDDAGFGETPYYTIDQPDDLARGSLPGVEAAVTFGPGDFERAAEALERLRPGEAKF
                     CGEYWAGWFARWGELPARDDSLAQTEDLARLLAGGWSVNVYMFHGGTNFGFHNGANFD
                     GSYRPVITGYDYRAALDQAGRPTPKYFAFRAAIGQSARIFPRPVPEAPRVVEVPAFAL
                     TESAPLESILTAPVTSASPRSMESLGQAFGYVLYRTELSEGGPGLLEIEEVRDFAVVC
                     VDGRPVAVLDRRLGGASARIEPAKRQSTLDILVENCGRVNYGPQIARERKGILGAVRW
                     NGRELRGWKIYSLPLADLSRLRFAPAKFSGAPAFFRGRFELAEPHDTFLNASGLGKGA
                     LFVNGRHAGRFWNLGPQRFLYVPGVWLRSGLNEVVTFDVLPQPEMVITGTPDYEGM"
     gene            complement(1905..>2561)
                     /gene="galT"
                     /locus_tag="VFU90_02905"
     CDS             complement(1905..>2561)
                     /gene="galT"
                     /locus_tag="VFU90_02905"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU5478750.1"
                     /translation="DLPPAALERVVDCWAEQYAQLAALPFVKAVTAFENRGAMMGASN
                     PHPHGQIWAESSVPSELAKETAGLTAFRRERGGCLLCAYAAAETGGERAIYDAGEVLA
                     VVPFWAVWPFEVLLLPRSHIGSLADLDGGLRAAFARAIGDLTRRYDRLFEAPFPYSMG
                     FHQRPCDGPHDEWHVHAHYYPPLLRSASVRKYSVGYEMLAQQQRDLTPEQAARLLREA
                     "
BASE COUNT          446 a          900 c          826 g          389 t
ORIGIN      
        1 gcgcgacggc agttcctcgc gccagaagcg ctccagcgag ccggcatctt ttccggcggc
       61 gatcaacgcc gcgttgatcg cgccgatcga gacgccgcaa atcacgtcga atcgctcgcg
      121 ctgcagcagc gactgggcca cgcccgcttc gtaggcgcca tgcgcgccgc cgccgcttag
      181 aaccagggcc ttcatcgtca cggttttccc cggctacatc ccttcataat cgggggtacc
      241 cgtaatcacc atctcgggtt gcggcagaac gtcgaaggtc accacctcgt tgagcccgga
      301 tctcagccag acgcccggca cgtacaggaa ccgctgcgga ccgaggttcc agaaccggcc
      361 ggcgtggcgg ccgttgacga acagcgcgcc cttgccgagt ccgctcgcgt tcaggaacgt
      421 atcgtgcggt tcggccagct cgaaccggcc gcggaagaac gccggcgccc cggaaaactt
      481 cgccggtgca aagcgaagcc gcgagagatc cgccagcggc agcgaataga tcttccagcc
      541 gcgcagctcg cgaccgttcc agcgcaccgc gccgagaatt cccttgcgtt cgcgcgcgat
      601 ttgcggcccg taatttacgc ggccgcaatt ctccaccagg atgtcgagcg tgctctgccg
      661 cttcgccggt tcgatccgtg ccgacgcgcc gccgagacgc cggtccagca ccgccaccgg
      721 ccggccgtct acgcagacga ctgcgaaatc gcgcacctct tcgatttcca gcaggccggg
      781 cccgccttcg ctcagctcgg tgcgatagag tacgtatccg aacgcctgac cgagcgattc
      841 catcgagcgc ggcgacgccg acgtaacggg ggcggtgagg atcgattcca acggcgccga
      901 ttcggtcagc gcgaacgcgg gaacttctac gacgcgcggc gcctcgggta ccgggcgcgg
      961 aaagatgcgc gcgctttgcc cgatcgcagc gcggaacgca aagtacttcg gggtggggcg
     1021 cccggcctga tcgagcgcgg cgcgatagtc gtagccggtg atcaccggcc ggtaactccc
     1081 atcgaaattg gcgccgttgt gaaatccgaa gttcgttccg ccgtgaaaca tatagacgtt
     1141 caccgaccag ccgccggcga gcaagcgcgc gaggtcctcg gtttgcgcga gcgaatcgtc
     1201 gcgggccggg agctcgcccc atcgcgcgaa ccatccggcc cagtattcgc cgcagaactt
     1261 cgcctcgccg ggacgcaacc gttcgagcgc ttccgccgca cgttcgaagt cgcccggccc
     1321 gaaggtgacg gccgcttcga cgcctgggag cgagccgcgt gcgagatcgt ccggctgatc
     1381 gatcgtgtaa tacggcgttt cgccgaaccc cgcgtcgtcg agcgcctcgc gcaacgcctg
     1441 caagtagcgc ggatcttcgc cgaacgcgcc gtactcgttc tccagctgca ccgcgacgat
     1501 cgggccgccg cgcgatcgct gcagttgggc gagttcttcg cccagacggt gcagccaccg
     1561 gcgaaccggc cgcatgaagc gctcgccggc cgtccgaagg ccgatcgaat cgtcggtcag
     1621 cagccacgcg gggagcccgc cgaggtccca ttccgcgcaa acgtatggcc ccgggcgcaa
     1681 aatcacgtcg agacccgcgg cggcggccgc gcgcacgtat tcggcgacgt cgttttcgcc
     1741 gtcgaaatcg taaacttcgg ggcgcggctc gtgcaggttc cagaagacgt aggtcgagac
     1801 ggcgtccagc cccatcgcgt acgccatttc taaacgcgcg ctccagtacg cacgcgggac
     1861 gcgcgcgtaa tgcatctcgc ccgaaatcaa gcggcgcttg ccgatcatgc ttcgcgcagg
     1921 aggcgtgccg cctgctccgg cgtgagatcg cgctgctgct gcgccagcat ttcgtagccc
     1981 accgaatatt tccgcacgct cgccgagcgc aacagcggcg gatagtagtg cgcgtggacg
     2041 tgccactcgt cgtgcggacc gtcgcacggc cgctggtgga atcccatcga atacggaaag
     2101 ggggcttcga agagccggtc gtagcggcgc gtgaggtcgc cgattgcgcg agcgaacgcc
     2161 gcccgcaggc cgccgtcgag atcggccagc gagccgatgt gcgagcgggg aagcagcagc
     2221 acctcgaacg gccagaccgc ccaaaacggc accacggcga gcacttcacc ggcatcgtaa
     2281 atggcacgct cgccgccggt ttcggcggcg gcgtaggcgc acagcaaaca gccgccgcgc
     2341 tcgcggcgga acgccgtaag accggccgtc tctttggcga gttccgacgg caccgacgat
     2401 tccgcccaaa tttggccgtg cggatgcgga ttgctcgcac ccatcatcgc tccgcgattt
     2461 tcgaaggccg tcaccgcctt gacgaacggc agcgcggcca gctgcgcgta ctgttcggcc
     2521 caacagtcga cgacgcgctc cagcgccgcc ggcggaagat c
//
LOCUS       DASUCJ010000235         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_140136, whole genome shotgun sequence.
ACCESSION   DASUCJ010000235 DASUCJ010000000
VERSION     DASUCJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140136"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFU90_02910"
     CDS             complement(<1..522)
                     /locus_tag="VFU90_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEU5478751.1"
                     /translation="MLPVDYAAHGLTSDEAERRLADVGPNDPLAVARESALREFAEPF
                     ANPLIITLLLASGISAYVGEVTSAAIIVLMVLLSVGLHLTQTYRSRRAVDRLRVSVAP
                     TATVERDGAWGELPRRLLVPGDVIRLTAGDLVPADARLVEAKDLHVHQAALTGEALPV
                     EKSAVPAAGDTGAD"
     gene            complement(489..1433)
                     /locus_tag="VFU90_02915"
     CDS             complement(489..1433)
                     /locus_tag="VFU90_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5478752.1"
                     /translation="MPTMRAKKSARRVRRQPGKTGRAHAALRLLVALDEASARANAPI
                     DTAMALTKAHRAKAEAVSVVDPMAATRLTIGVPVAGAREAALRGEQRLRRLELRRYVA
                     DRTGARPPWRIRVPLGTPADAILDAANGADLVIMGLRREGLIERIVHDATTPHVVREC
                     SVPVLAVTPELRGMPRTVVIAADFSPESNAAAQLACRLFTGCARLVFVHIESDFDAAI
                     ANLDEITRATRENDWAVAFQRLIETLSVPAGAKVETVVVHGTVPRTLLAFAKRRGADV
                     LVVGRQQHDVAERVLVGSVTAGLVRRAPCSLLITPPTV"
     gene            1765..>2241
                     /locus_tag="VFU90_02920"
     CDS             1765..>2241
                     /locus_tag="VFU90_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478753.1"
                     /translation="MFRTLRNALAGRFAADLVAALAIIGAVWLAQPVAGLVIVLMQTG
                     GEALERFAEGRASDAVRALEEAAPRTAHRIDGTRLDDITADAIRVADVLLVRPGELMP
                     CDAIVVDGRSHVDVSRLTGEPLPVVATPGTRLMSGTVNGEGTLTVRAVATAGESQYA"
BASE COUNT          342 a          767 c          771 g          361 t
ORIGIN      
        1 gtccgcaccg gtgtcgccgg cggccggcac cgcgctcttt tcgaccggga gcgcttcgcc
       61 ggtgagggcc gcctggtgca cgtgcaggtc cttggcttcc acgagccggg catcggcggg
      121 cacgagatcg ccggccgtta ggcggatcac atcgcctggc acgagcagcc ggcgcggcag
      181 ctcgccccag gccccgtcgc gctcgaccgt tgccgtcggc gccacactca cgcgcagccg
      241 gtcgacggcc cggcgcgacc gatacgtttg ggtgagatgg agcccgacac tgagaaggac
      301 catcagcacg atgatagcgg cgctcgtgac ctcacccacg tacgccgaaa tgccgctggc
      361 gagcagcagc gtaatgatga gcggattcgc gaacggttcc gcgaattcgc gcagggccga
      421 ctcgcgcgcg acggcgagcg ggtcgttcgg cccgacgtcg gcgaggcggc gctcggcctc
      481 gtccgacgtc agaccgtggg cggcgtaatc aacagggagc acggcgcacg gcgcacgagt
      541 ccggcggtca cagaaccgac gagcacgcgc tcggcgacat cgtgctgttg tcggccgacc
      601 acgagcacgt cggcgccacg acgcttggcg aaggcgagca gcgttcgagg cacggtgccg
      661 tgcactacca ccgtctccac ttttgcgccg gctggaaccg acagcgtctc aatgagacgc
      721 tggaacgcca cggcccaatc gttctcgcgg gtcgccctcg tgatctcatc gagattcgcg
      781 atggccgcgt cgaagtcgga ctcgatgtgc acgaaaacca gtctcgcgca ccctgtgaac
      841 aacctgcacg ccaactgcgc ggcggcgttg ctttccggac tgaagtcggc cgcgatcacg
      901 acggtgcggg gcattccgcg cagctccggt gtcaccgcca gcacggggac gctgcactcg
      961 cgcacgacgt gcggcgtcgt cgcgtcgtgg acgatccgct cgatgagccc ctcgcggcgc
     1021 aagcccatga tcaccaggtc cgcgccgttc gcggcatcca ggatggcgtc ggccggagtg
     1081 cctaacggaa cgcgaatgcg ccacggcggc cgggcgccgg tgcggtcggc tacataccgg
     1141 cggagctcga gccgccgaag ccgctgctcg ccgcgcaacg ccgcctcgcg ggcgccggcg
     1201 accggcacgc caatggtcag ccgcgtcgct gccatggggt cgacgacgct caccgcttcg
     1261 gctttcgcgc ggtgcgcttt cgtcaacgcc atcgcggtat cgatcggggc attggcgcgc
     1321 gcgcttgctt cgtcgagtgc gaccagcaat cgcagtgctg catgcgctct gcccgtcttg
     1381 cccggctgcc gacgcacgcg tctggctgat ttcttcgctc gcatcgtagg catcgttcct
     1441 cctcgtagtc ggtatgttgg gcatacgcta cgtgctgccc gctcccgggt ctgtcgggga
     1501 agtgccgagc ctgggtcacg gaagccctga cagcttccgg actccgacac agcattattg
     1561 tcaagacgca aacggagtcg aaggacgcca tcgatggcag gtcatgacgc acacagaacg
     1621 cggcaatggt tgaccagcac ggccgtgccc gcggccgccc tcttgctcct cggcgcgggc
     1681 gccgtggcac gctttgggtg gccgacagcg ggggcgagca catccatttg gaccatcggc
     1741 ttagtgctca caggtgcgcc gacggtgttt cgtacgctgc gcaacgcgct cgccggtcgc
     1801 ttcgccgcgg acctcgttgc cgcgctggcg atcatcggcg cggtctggct cgcccaaccg
     1861 gtggccgggt tggtgatcgt cctcatgcag accggcggcg aggctctcga gcgctttgcc
     1921 gaaggacgcg catccgatgc cgtgcgcgcg ctggaggaag cggctccacg cactgcgcat
     1981 cggatcgatg gtacgcgtct cgacgacatc accgcggacg ccatccgagt agctgacgtc
     2041 ctcctcgtta ggcccggcga attgatgccg tgcgatgcaa tcgtcgtcga tggccgctcg
     2101 cacgtcgatg tgtctcgtct gacgggcgaa ccgctgccgg tggtggcgac gcccgggacg
     2161 cggctcatga gcggtaccgt gaacggcgaa ggcacgttga cggtgcgcgc ggttgccacg
     2221 gcgggtgaaa gccagtacgc g
//
LOCUS       DASUCJ010000236         1983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1041671, whole genome shotgun sequence.
ACCESSION   DASUCJ010000236 DASUCJ010000000
VERSION     DASUCJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041671"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFU90_02925"
     CDS             <1..626
                     /locus_tag="VFU90_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEU5478754.1"
                     /translation="WIGELIWRDFYQMVLKTWPQVANGPFLARAAQIPWRTADVEFDA
                     WCAGRTGYPIVDAAMRQLNEYGWMHNRLRMIVASFLTKDLLVDWRRGERYFERHLADA
                     DLAANNGGWQWSASTGTDAVPYFRIFNPVVQSRRIDPEGRFIRRMIPELRRLPDKFVH
                     APWELPSSLASEAAFRIGVDYPAPMVEHGAARIRALQAFSALKGEKS"
     gene            complement(623..1303)
                     /locus_tag="VFU90_02930"
     CDS             complement(623..1303)
                     /locus_tag="VFU90_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5478755.1"
                     /translation="MFILIVGGGKVGSYLTRALVNQQHEVVVVEKDARKAKTLERLVD
                     RQVTVVGDGCDPAVLEEAGVARADVVVADTGDDEDNLVVVLLSKKKSKARCIARVNNP
                     ANKLIFESLDTEDPVTVISSTELILDVLNERVNAAKYRSMLETMHLFGKGDMQLVKVA
                     IPERSPVAGKSLAELKLPHNSVVVAIDRPEHDLEIPTGDSTLRAGDSMIVIVKNGAAE
                     RIRELLAG"
     gene            complement(1308..>1983)
                     /locus_tag="VFU90_02935"
     CDS             complement(1308..>1983)
                     /locus_tag="VFU90_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5478756.1"
                     /translation="MRYLIVGCGRVGSALAKYLDADGHEIIVVDENAAAFKRLGPKFK
                     GHVVVGTGIDYDVLKRAGAATADGFIAVTNGDNRNIMAALIAQRMFKIKRIVARIYDP
                     PRGQMYRELGVATVCPTTVGAKMIRDVLMEAPWNSLQSFDYGKVTSLAAVVTRAEAGK
                     AVKDIEDAGRIRIAAIRRASGGVVVASADAILEEGDEINAVVAPEAISEFAQRFGTAA
                     PTQLTA"
BASE COUNT          331 a          617 c          645 g          390 t
ORIGIN      
        1 cgtggatcgg cgagctgatc tggcgcgatt tctaccagat ggtgctcaag acgtggccgc
       61 aggtcgcgaa cggcccgttc ctcgcgcgcg ccgcgcaaat accctggcgt acggccgatg
      121 tggaattcga cgcgtggtgt gccggccgca ccggctaccc gatcgtggat gcggcaatgc
      181 gccagttgaa cgagtacggc tggatgcaca accggttgag gatgatcgtc gcgtcattct
      241 tgaccaagga tttgctcgtc gactggcgtc gcggcgaacg gtatttcgaa cgccatctgg
      301 cggacgcgga tttagcggct aacaacggcg gctggcagtg gtcggcctcg accggaaccg
      361 acgccgtccc ctattttcgc atcttcaatc cggtcgtaca aagccgacgg atcgatccgg
      421 agggcaggtt cattcgacgg atgattccgg agctgcgccg cctgccggac aagttcgtgc
      481 acgcgccgtg ggagctgccg tcctcgctcg cgagcgaagc ggccttccgg atcggcgtcg
      541 actatcccgc gccgatggtc gagcacggag cggcccgcat ccgcgcgctg caagcttttt
      601 cggcgttgaa aggggagaag agctagcccg cgagcagttc gcggatacgc tcggccgcgc
      661 cgttcttgac gatgacgatc atcgagtcgc cggcgcgcag cgtggaatcg ccggtcggaa
      721 tctcgaggtc gtgctccggg cggtcgatcg cgaccacgac cgagttgtgc ggaagcttca
      781 actccgccaa gctcttgccc gcgaccggcg agcgctcggg gatcgcgacc ttgacgagct
      841 gcatgtcgcc tttaccaaaa aggtgcatcg tttccagcat cgagcggtat ttcgccgcgt
      901 tgacgcgttc gttgagcacg tcgaggatca gttccgtcga agaaatgacg gtcacggggt
      961 cctcggtatc cagcgattcg aagatcagct tgttggcggg attgttcacg cgggcgatgc
     1021 agcgcgcttt ggatttcttc ttggagagaa ggacgacgac cagattgtct tcgtcgtcgc
     1081 cggtgtcggc gaccaccacg tcggcgcggg cgacgcccgc ttcctcgagc acggcgggat
     1141 cgcagccgtc tccgacgacc gttacttgac ggtcgaccaa acgttcgagg gttttggctt
     1201 tgcgcgcgtc tttttcgacg acgacgactt cgtgctgctg gttcacgagc gcgcgcgtca
     1261 gataggatcc gaccttgccg cccccgacga tcaaaataaa catggcgtta tgcggtcagc
     1321 tgcgtgggcg cggcggtccc gaagcgctgc gcgaactcgc tgatcgcttc gggtgcgacg
     1381 accgcgttga tctcgtcgcc ttcctccagg atcgcgtcgg ccgacgcaac gacgacgccg
     1441 cccgacgcgc gccggatcgc cgcgatccgg atgcgtccgg catcctcgat gtccttgacg
     1501 gccttgcccg cctccgcacg cgtgacgacg gccgccagcg acgtaacctt tccgtagtcg
     1561 aacgactgca gcgagttcca cggcgcttcc atcagcacgt cgcggatcat cttggcaccg
     1621 accgtcgtcg ggcacacggt cgcgaccccg agttctcgat acatttgtcc gcgcggcggg
     1681 tcgtaaatgc gcgcgacgat gcgcttgatc ttgaacattc gttgggcgat cagcgcggcc
     1741 atgatgttgc gattgtcgcc gttggttacc gcgatgaatc catcggcggt tgccgcaccg
     1801 gcgcgcttga ggacgtcgta gtcgatgccg gttccgacga cgacgtgtcc cttgaatttg
     1861 gggccgagac gcttgaaagc ggcggcattt tcgtcgacga cgatgatttc atggccgtcg
     1921 gcatcgaggt acttcgccag tgcggacccg acgcgaccgc acccaacgat gaggtagcgc
     1981 atg
//
LOCUS       DASUCJ010000237         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1072461, whole genome shotgun sequence.
ACCESSION   DASUCJ010000237 DASUCJ010000000
VERSION     DASUCJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072461"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /locus_tag="VFU90_02940"
     CDS             complement(<1..>1241)
                     /locus_tag="VFU90_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478757.1"
                     /translation="AVLSLGLALAVNTTIFALADGVMHPYVVYSHPERIIVPTFMGGD
                     PRHALSFDIRYRAIRDGLHAYDKIAAYTVLQAAVQTGTGGRDWAPVGVSARFFDVLGV
                     RPSLGRAIDTSDVGTGATQAAVISYRLWSGEFDRRPLGDSLTLEIDRTRYTVVGVMPR
                     GVHFPSGFTDVWIPIDALPGASRHFGPFAVVHLRKGASRETLWSELAIVSKRLTAEYT
                     PKRPLSPRLFPLEVDGRATATPFPPLFSRSVLIVLVIACANLATMLIARGLARRRETA
                     IRMALGASRAAIVRGVLAECGLIVAGSVAVGALLTAWALYVVPHFTTPFVPQLGDIDP
                     TPRWRVFVYALEVAIATVLVAAVVPAIRAASIDPSEPMKDGAGTTTGRQRHRYNPLIM
                     VEVALSTTLLMCSALFTIVVVR"
BASE COUNT          241 a          395 c          434 g          171 t
ORIGIN      
        1 cgaaccacca cgatggtaaa gagcgccgag cacatgagga gtgtggtcga gagcgcgacc
       61 tcgaccatga tgagcggatt gtaacggtgg cgctgtcgcc ccgtcgtcgt ccccgctccg
      121 tccttcatcg gctcggacgg gtcgatggac gccgcgcgaa tggccggaac cacggccgcg
      181 acgagcaccg tcgcgatggc gacctccagt gcgtacacga acacgcgcca ccgcggcgtc
      241 ggatcgatgt caccaagctg cgggacgaat ggagtcgtga aatgcgggac gacgtacagc
      301 gcccacgccg tgagcagggc gccgaccgcc acgctgccgg cgacgatcaa cccacattcg
      361 gcgagaacgc cacgcacgat tgcggcgcgt gaggcgccga gggccattcg gatcgccgtc
      421 tcacgccggc gcgccagtcc gcgggcgatg agcatcgtgg cgaggttggc gcacgcgatg
      481 accaggacga tcaggaccga tcgcgagaag agagggggaa agggcgtggc cgtggcgcga
      541 ccgtccacct cgagggggaa caaacggggg gacaggggcc gcttcggcgt gtactcggcc
      601 gtcaggcgct tgctgacgat cgccagctca gaccacaggg tctccctcga agctcccttg
      661 cgaagatgta cgaccgcgaa tgggccgaaa tgtcgggacg cacccggcag cgcgtcgatc
      721 ggaatccaga cgtcggtgaa gcccgaggga aagtgaaccc cgcgcggcat cacgccgacg
      781 accgtgtagc gagtgcgatc gatctcgagg gtgaggctat cgccgagcgg gcgtcgatcg
      841 aactcgccac tccacagtcg atagctgatg acggcggcct gagtcgcgcc ggtgcctacg
      901 tcgctcgtgt cgatcgcgcg accgagggac ggtcggacgc caagcacgtc gaagaaccgt
      961 gccgatacgc cgaccggtgc ccagtctctg ccgccagtgc ctgtctgcac ggccgcctgc
     1021 aacaccgtgt acgcggcaat cttgtcgtac gcgtgcaggc cgtcgcgaat cgcgcgatac
     1081 cgaatgtcga agctgagtgc atgtctggga tcaccaccca tgaacgtcgg gacgatgatg
     1141 cgctcggggt gcgagtagac gacgtagggg tgcatcacgc catcggcgag cgcgaagatc
     1201 gtcgtgttca ccgccagcgc gagaccgagc gagagcacgg c
//
LOCUS       DASUCJ010000238         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_598419, whole genome shotgun sequence.
ACCESSION   DASUCJ010000238 DASUCJ010000000
VERSION     DASUCJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598419"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFU90_02945"
     CDS             complement(<1..511)
                     /locus_tag="VFU90_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5478758.1"
                     /translation="MPSPTIKVAAAHAAPVYMNRAATIEKALSIIDEAARNGAELVVF
                     PESYVPGFPVWAALRSPIDNHDWFKRMVANSIVVDGPEMAKIRAAAKRNGVFVSIGIS
                     ESTRASVGCIWNSNVLIGDDGGILNHHRKLVPTFFEKMVWASGDGHGLVVSDTRIGRL
                     GALICGENTN"
     regulatory      558..661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            755..>1049
                     /locus_tag="VFU90_02950"
     CDS             755..>1049
                     /locus_tag="VFU90_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEU5478759.1"
                     /translation="MEAFLVSAGVVALAEIGDKTQLIALVLAARFKRPLPVIAGIVAA
                     TVVNHTIAGIVGAWLASVLGPAALRWILGLSFVAIAVWLLIPDKVDSADVARAR"
BASE COUNT          154 a          361 c          332 g          202 t
ORIGIN      
        1 gattggtgtt ctcgccgcag atcaacgcgc caagccggcc gatgcgcgtg tcgctcacga
       61 cgagcccgtg gccgtcaccg cttgcccaca ccatcttctc gaagaacgtg ggcacgagct
      121 tgcggtgatg attgaggatc ccgccgtcgt cgccgatcag cacgttgctg ttccagatgc
      181 agcccacgct cgcgcgcgtc gactcgctga tgccgatcga cacgaaaacg ccgttgcgct
      241 ttgccgccgc acgaatcttc gccatctccg gtccgtcgac gacgatgctg ttcgcgacca
      301 tccgtttgaa ccagtcgtgg ttgtcgatcg gcgaccggag ggccgcccac accggaaatc
      361 ccgggacgta ggactccgga aagacgacga gctcggcgcc gtttcgcgcc gcctcgtcga
      421 tgatcgacag cgctttctcg atcgttgctg cgcgattcat gtagacgggt gctgcgtgtg
      481 cggcagcaac cttgatcgtc ggtgagggca tgcggtttct ccgttacggc gagcggccga
      541 tcgctgagtt atactcccgc ccgctcatcg gggagtagtc accgccggcc tccggcgggg
      601 ctgtgtcaac acacttggtc gcaactgacc atggcgcagc cggcacggta tcgcgcgtgc
      661 tcgacgagac ctttggcgcg gcacaccgct cacggtcggg cggagggtgc cgccgccatc
      721 ggttgtcgaa gccgcccgac cagacaggtg cccgatggaa gcctttctcg tctccgccgg
      781 cgtcgtcgcg ctcgcggaga tcggcgacaa gacgcaactg atcgcgctcg tcctcgccgc
      841 gcgattcaag cgcccgctcc cggtcatcgc cggcattgtc gcggcgaccg tcgtcaacca
      901 tacgatcgcc ggcatcgtcg gcgcgtggct cgcttcggtg ctcggcccgg ctgcgctgcg
      961 ctggatcctg ggactctcgt tcgttgccat tgcggtgtgg cttctcatcc ccgacaaggt
     1021 cgactccgcc gacgttgcgc gagctcgct
//
LOCUS       DASUCJ010000239         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_63624, whole genome shotgun sequence.
ACCESSION   DASUCJ010000239 DASUCJ010000000
VERSION     DASUCJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63624"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1006
                     /locus_tag="VFU90_02955"
     CDS             155..1006
                     /locus_tag="VFU90_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478760.1"
                     /translation="MKRLAPSLVVVIGASLAIALGLWSEVRAGTLPDISGTWYANGNR
                     AFACRISQSGSSVSLTNEQGATATGQFVDPSSLTTNWGIFAGGTITGRISGDLRIITW
                     NNGTYWSRPSAPTTTGNVASGYVAPTPAPTPKPYRYLRWTAAEGARAPIDYVDGWTAV
                     MRNGSMVYACVSFKNTSGVTATRIRFDFPLSNQDGQVVDTARLDRKGTFSPNVEIHGW
                     HDLAEWSSGQGHRGYYDNCVRWKPDNEEERLAYSRLQHYSIRVVHIDFTDGTAWPVAE
                     PEPSPSP"
     gene            1134..2267
                     /locus_tag="VFU90_02960"
     CDS             1134..2267
                     /locus_tag="VFU90_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478761.1"
                     /translation="MPIRTHAQFITGAAMAALFLGACSGGQNAAPGMPPGAAPAQLSP
                     AAGPKGSGILSQLKNQVVIGSTIDPVYGQLNPYGLTVAPSTSGAFTKGDLAVCNFNAS
                     SNVQGTGYTIVALHPQPGSSPVLVAADPTTLLGCDAIAMTPDDTIWAAAFAANDNPAF
                     SSSGTLVTNLSGAPFNSPFGQTYAQPMKGGAAIYESNAGDGSIVRINLGGSITTQVVA
                     TGFPVNGGAPGSILGPSGLQYDRRHDTLYVVDGQDNSVTAISKVSTLPAGCLRVMQRG
                     QKFGGHCAARARVLHSGAPLNGPISSALLFNGDLVIGNTLDPNGENLMVELNPAGKVL
                     AVRNVDTGASGSLFGMVATGNNAANTKLYFNDDNDNNLQVLER"
     gene            2402..2962
                     /locus_tag="VFU90_02965"
     CDS             2402..2962
                     /locus_tag="VFU90_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5478762.1"
                     /translation="MSKLIYITNASLDGYVEDATGAFDWVNPEQVHEFITEMLRPIGI
                     HLYGRRLYETMAYWETPLERFPPEHRDFAQVWQNSEKIVFSRTLTGATTGNTRVERDF
                     DLVAIRKVKRESERDIVIGGAELAGLALEAGLVDECHLFLNPVILGGGKPAFRAALRR
                     NLELLETRHFGTGVVHLRYRVRGDAP"
BASE COUNT          614 a         1062 c          952 g          579 t
ORIGIN      
        1 tcaccgtgta tgccgccggc ggcagctcgc cgttgcgaac gattacacag ggcatccacc
       61 ggccggttgc cgccgcactg ggaccttaaa gcctcggccg agggaacggt cgcctgctcg
      121 cgaagctttc ttcgcacaca ataaggaggc ggctatgaaa cgcttagcac cttcactcgt
      181 cgtcgttatc ggcgcgagct tggccatcgc tctcggtttg tggagcgagg tgcgcgccgg
      241 cacactcccg gacatcagcg gcacctggta tgccaacggc aaccgcgcct ttgcctgccg
      301 catcagccaa agcggcagct cggtgtcgct gaccaacgaa cagggcgcaa ccgcgacggg
      361 ccaattcgtc gatccaagct cgctcaccac gaactggggc atattcgccg gcggcaccat
      421 taccggccgc atttcgggcg acctgcgaat cattacgtgg aataacggga cgtattggtc
      481 tcgcccatcc gcacccacaa ccacggggaa cgttgcctcc ggatacgtcg cgcccacgcc
      541 cgcgccgacg ccgaagccgt accggtattt acgctggacc gcggccgagg gagcgcgcgc
      601 gccgatcgac tatgtcgacg gctggacggc cgtcatgcgc aacgggagca tggtgtacgc
      661 ctgcgtttcg tttaagaaca cgagcggcgt tacggcgacg cgaatccgct tcgattttcc
      721 gctctcgaat caagatggcc aagttgtcga tacggcccgc ctcgatcgca agggcacgtt
      781 ttctcccaac gttgagattc acggctggca cgatctcgcg gagtggtcga gcggccaagg
      841 gcatcgcggc tactacgaca actgcgtgcg ctggaagccc gacaacgaag aggagcgact
      901 agcctattcg cggctccagc actactcgat tcgcgtcgtg cacatcgatt tcaccgacgg
      961 aaccgcatgg ccggttgcgg aacccgaacc gagtccttcg ccgtaatagt tgccatgtga
     1021 gtgcaagcga gcgatccgca cacaccccgc acgcttcccg cacgcttcag actttcttca
     1081 gacccgcgaa cgagactcgg gatacccttt tctcaacatc gcgaggtgct tcgatgccaa
     1141 tacgcacgca tgcgcaattc atcaccggcg cggcgatggc ggcgcttttt ctcggcgcct
     1201 gcagcggcgg tcaaaacgcc gctccgggga tgcctccggg agcggcgccg gcccaactct
     1261 cgccggcggc cgggcccaaa ggctccggta tcctaagcca actcaagaat caagtcgtca
     1321 tcggttccac catcgatccg gtatacggtc aactcaaccc gtacggcctg accgttgcgc
     1381 cctcgacgtc gggtgcgttc accaaaggcg acctagcggt ctgtaacttc aacgcgagca
     1441 gcaacgtcca gggcaccggt tacacgatcg tcgccctcca tccgcaaccc ggctcgtcgc
     1501 cggtgctcgt tgcggcggac ccgacgacgc tcctcggctg cgacgcgatc gcgatgacgc
     1561 ccgacgatac gatttgggcg gcggcgttcg ccgcgaacga caatccggcg ttctcctcga
     1621 gcggaacgct cgtgacgaac ctctccggag cgccgttcaa ctccccgttc ggccaaacgt
     1681 acgcgcaacc gatgaagggc ggcgcggcaa tttacgagag caacgccggc gacggatcga
     1741 tcgtccgcat caatctcggc gggtcgatca ccacccaagt cgtcgcaacg ggtttcccgg
     1801 tcaacggcgg ggcgcccggc agcattctcg gaccgtccgg cctgcagtac gaccgccggc
     1861 acgacacgct ctacgtggtt gacggccaag acaactcggt cacggcaatt tcgaaggtat
     1921 ctaccctgcc ggccggctgt ctgcgggtga tgcagcgcgg ccagaaattc ggcgggcatt
     1981 gtgcggcgcg cgcccgcgtg ttgcacagcg gggcaccgct gaacggtccg attagttcgg
     2041 cgctgctctt caacggcgat ctcgtcatcg gcaatacgct cgatccaaac ggcgagaacc
     2101 tcatggtcga actgaacccg gccggaaaag tgctggccgt gcggaacgta gacaccggcg
     2161 cctccggctc cctcttcggc atggtcgcca ccggcaacaa cgcggcgaat accaaactgt
     2221 acttcaacga cgataacgac aacaatctgc aggtgctcga gcggtagccg cccgctccaa
     2281 ccctccgtcg ccgggccgca gcagcgctgc agcccggcga cgcaaggagc atcgcatcgc
     2341 ctcgcggggg cgtgcagttt gcgggcaggg ccgccgtaac gaatcgtcct acttcttccg
     2401 catgtcgaag ctgatctata ttacgaacgc gtcgctcgac ggttacgtgg aggacgcgac
     2461 tggggccttc gactgggtta atcccgaaca ggtacacgag ttcatcacgg agatgttgcg
     2521 gccgatcgga attcatcttt acgggcggcg actctacgag acgatggcct actgggagac
     2581 gccgctggag cgcttcccac ccgaacaccg cgacttcgca caagtctggc aaaactccga
     2641 gaagatcgtc ttttcacgaa cgctgaccgg cgcgacgacg ggcaacacgc gcgtcgagcg
     2701 ggacttcgac ctcgttgcta tccggaaggt caagcgggag tcggagcgcg acatcgtgat
     2761 tggcggggcc gagctcgcgg gacttgcgct cgaagccggc ctcgtcgacg aatgtcactt
     2821 gtttctcaac ccggtgatcc tcggcggcgg aaagccggca ttccgcgccg ccctacggcg
     2881 aaatctcgaa ctcctcgaga cgcgccattt tggtaccggg gtcgttcact tgcgctatcg
     2941 cgtaagaggc gatgctccat agagcgctct ccagttcgca actcgtatct aggtcgtcga
     3001 gccgtctcag ccaagcaagt gctgggcttc ccgcgattct tctgctattg acattgcttg
     3061 cggcacaatg caccgttgtg tcaacgcgat ttgcatccaa cagcgttatc gtcgcgggca
     3121 ccgtagatta tccgtgcaac gcgtagcctg tacgtcgtct taatctagga ggcgtctcca
     3181 tgccggaaaa cgttcgtctc gatcgcc
//
LOCUS       DASUCJ010000240         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_736217, whole genome shotgun sequence.
ACCESSION   DASUCJ010000240 DASUCJ010000000
VERSION     DASUCJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736217"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFU90_02970"
     CDS             <1..315
                     /locus_tag="VFU90_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478763.1"
                     /translation="LAALFPAAVTRPIALHVMAKRYRCAVDATEFGRLSNGSQASLAR
                     QGGPLDPAMAARFEGVDGFHDATTLRGWDDAGKLPELTVPPLADYIALLARVSTSHAE
                     PS"
     gene            complement(264..1100)
                     /locus_tag="VFU90_02975"
     CDS             complement(264..1100)
                     /locus_tag="VFU90_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478764.1"
                     /translation="MSVLVVMAALALVLRPRRRRASATADLSIVVPATGRSTLVGNFL
                     LDAAIDFLDIAHHGVAWRAAERHEETGIPFADDRLPSYFAGPFDAPIVLVHMMPKYEA
                     NESDRYRDFDDYVEQHRRWTADDTSRSAKRARDFTSAFTQGARLDLYFIPYAARRFDT
                     RNLDGRLFRKEYERIMQMIDAYPRDYVVFCGAAFDRLLAPYIIEKDDQRFRVSTRDGI
                     SKAQYRSSAVTLRFRQRPVRAAIAQSFTLTTTPMRAYGEACRDRYEGSACDVDTRASS
                     AM"
BASE COUNT          230 a          427 c          462 g          244 t
ORIGIN      
        1 ctcgccgcac tgttccccgc cgccgtgacg cggccgattg cgctacacgt gatggcgaag
       61 cgttatcggt gcgcggtcga cgcgaccgag ttcgggcggc tctcgaacgg ctcgcaggcg
      121 agcctcgccc gtcagggcgg accgctcgac cccgccatgg ctgcgcgttt cgaaggggtc
      181 gacgggttcc acgatgcgac gacgctgcgc ggttgggacg atgccggaaa gttgcccgag
      241 ctgacggttc cgcccctggc cgactacatc gcgctgctcg cccgcgtgtc cacatcgcac
      301 gccgagcctt cgtagcgatc gcggcacgcc tcgccgtagg cgcgcatcgg tgtcgtagtc
      361 aacgtgaacg attgcgcgat cgccgcccgc accggcctct gccggaaacg gagtgttacc
      421 gcgctcgagc ggtactgcgc tttgctgatt ccgtcgcgcg tcgacacgcg aaagcgctga
      481 tcgtccttct cgatgatgta cggcgcgagc aggcggtcga acgccgcacc gcagaacacg
      541 acgtagtcgc gtggatacgc gtcgatcatc tgcatgatgc gttcgtattc cttccggaac
      601 aaccggccgt cgaggttgcg ggtgtcgaag cgtctcgccg cgtatgggat gaagtacagg
      661 tcgagacgcg cgccctgcgt gaatgcgctc gtgaagtcgc gtgcgcgctt cgccgatcgc
      721 gaggtgtcgt ccgcggtcca tcgacgatgc tgctcgacgt agtcgtcgaa atcgcgatag
      781 cggtccgatt cgttggcttc gtacttcggc atcatgtgga cgaggacgat cggcgcgtcg
      841 aaaggtcctg caaagtaact cggcaagcga tcgtcggcga atggaatgcc cgtttcctcg
      901 tgacgctcgg ccgcccgcca cgcaacaccg tggtgtgcaa tgtcgaggaa gtcgatcgcg
      961 gcgtcgagga ggaagttgcc cacgagcgtc gagcgccccg tcgcgggcac gacgatgctg
     1021 agatcggcag tcgcggaagc gcgccgccgt cgtgggcgga gcaccagcgc aagggccgcc
     1081 atcacgacaa gcactgacac gacgccggtg acgacctgtg cctcattgtt gaggctcgcg
     1141 aaccgcgaca tccacgaccg caacagagtc gtggatgtcg cgttcacgtg cactggctat
     1201 gacgacagtc cgctcggcga gacgaaggtg tagccgagcc ccttgatgcc gtcgatcgtt
     1261 tggtcgagcg cagatggtcc ttctgacggg tcgtagaaga agctggccca tccgtcgcga
     1321 atcgcgaggt tgagctgcgc gttgtggatg atgtcggcgg gaa
//
LOCUS       DASUCJ010000241         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_873664, whole genome shotgun sequence.
ACCESSION   DASUCJ010000241 DASUCJ010000000
VERSION     DASUCJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873664"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFU90_02980"
     CDS             <1..1190
                     /locus_tag="VFU90_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5478765.1"
                     /translation="GTTAAEQLRKHEPSCEIVLFGDEPYTLYNRISLPPMLRRQIPEA
                     KVMIRNVAWHEEHRIELHLRTRVDRIVPEERIVEAEGKAYPYDALLIATGGRPNPTGK
                     PGGEGAQNLFPFQYLDDTRAISEQIDRSRAAVAIGGSFIAYELAEAFSSRGIETHWLM
                     RGPRGLHRIIDEAAGELLHDAALADGVHMHYGEEVGEFVRSNGVITKVRTANGMEIEA
                     QCYAYGFGLSMNTELCDGTGIETSKNGILCDDRLETNVKGVFAAGDVADFYDPILEMR
                     YRMGTWNNAGAHGKVAAINMMGGSELYHDVPEYSSLLFKGQTITQFGMGTDLRPDLEM
                     VRKVDREKKWYRALYFWQDRLVGGLMLGKGNRAGKRKYVEAIKNKQTFPKPQWEAMLD
                     WTA"
     gene            1231..1764
                     /locus_tag="VFU90_02985"
     CDS             1231..1764
                     /locus_tag="VFU90_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5478766.1"
                     /translation="MKYAVALACAALVTIGPAAAQTAAAPTQSPADDALQKALSSNYA
                     LGCTAAFNPTDANLEAAFASLAPDFVNIDPSGRQASRDEVVGLAKQQLKMLHAKSCDH
                     TIESIAPSADGTVVAVETLHLEGALQSGGGTHDLDLTDKAQDTWKNVDGKWQETKSKD
                     LHLLVKVDGNVVQDQSS"
BASE COUNT          443 a          696 c          695 g          355 t
ORIGIN      
        1 ccgggaccac tgccgcagaa cagctgcgca aacacgaacc ttcgtgcgag atcgtgctgt
       61 tcggcgacga gccgtacacg ctgtacaacc ggatctcgtt gccgccgatg ctgcgccggc
      121 agattcccga ggccaaggtg atgatccgca acgtcgcttg gcacgaggag caccggatcg
      181 agctgcacct gcgtacgcgc gtcgatcgga tcgttcccga agagcggatc gtcgaggccg
      241 aggggaaggc gtacccgtac gacgccttgc tgatcgctac cggcggccgc cccaacccca
      301 ccgggaaacc cggcggcgag ggcgcgcaga acctgttccc gtttcagtat ctcgacgaca
      361 cgcgggcgat ctcggagcaa atcgatcgca gccgggcggc cgttgcgatc ggcggctcgt
      421 tcatcgccta cgagctggcc gaggcgtttt cgtcgcgcgg gatcgagacg cactggctga
      481 tgcgcggtcc gcgcgggctg caccgcatca tcgacgaggc ggcgggcgag ctcttgcacg
      541 atgcggcgct ggccgacgga gtgcacatgc actacggcga ggaagtcggc gagttcgttc
      601 gctcgaacgg cgtgatcacg aaggtacgca ccgcgaacgg catggagatc gaagcgcagt
      661 gctacgcgta cggcttcggc ctgagtatga acaccgagct gtgcgacggc accgggatcg
      721 agacgagcaa gaacggcatt ctgtgcgacg accgcctcga aaccaacgtc aaaggcgtgt
      781 ttgcggcggg cgacgtcgcc gacttctacg acccgattct ggaaatgcgc taccggatgg
      841 gcacctggaa caacgcgggc gcgcacggaa aggtcgcggc gatcaacatg atgggcggca
      901 gcgaactcta ccacgacgtt ccggagtatt cgtcgctatt gttcaagggc caaacgatca
      961 cgcagttcgg catgggaacg gacttgcgcc ccgacttgga gatggtccgc aaagtcgatc
     1021 gcgagaagaa gtggtaccgg gcgctgtact tctggcagga tcgcctggtc ggcggcctga
     1081 tgctcggtaa gggcaaccgg gcgggtaagc gcaagtacgt cgaagccatc aagaacaagc
     1141 agacgttccc caagccgcaa tgggaagcga tgctcgactg gacggcctaa ctccgaggag
     1201 ccgcgtacgc cgctgcaaaa gggtgcgccg atgaagtacg cagtcgcgct ggcatgcgcc
     1261 gctctggtta cgattggacc cgcggccgct caaacggctg ccgctcctac gcaaagcccg
     1321 gccgacgatg cgctccaaaa agcgctgtcg tcgaactacg cgctcggatg cacggcggcg
     1381 ttcaatccta ccgacgccaa cctcgaggcc gccttcgcaa gtctcgctcc cgattttgtc
     1441 aacatcgacc cgagcggccg ccaagccagc cgcgacgagg tcgtcggctt ggcgaaacag
     1501 cagttgaaga tgctgcacgc caagagctgc gatcatacca tcgaatcgat cgccccttcc
     1561 gccgacggta ctgtcgtcgc cgttgagacc ttgcatctcg agggagccct gcagtcgggc
     1621 ggcggcaccc acgatttgga tctgaccgac aaagcccaag acacgtggaa aaacgtcgac
     1681 ggtaagtggc aagagaccaa aagcaaggac ttgcatcttc tggtcaaagt cgacggtaac
     1741 gtcgttcaag accaaagctc gtaactccac cgccgtcacc tgagcggagt gcccgtgtca
     1801 tcctgagcgg agctcccgtg tcatcctgag cggagcgccg caggcgcgca gtcgaaggac
     1861 gcgccgtgtt cgctccagcg gtgtcatcct gagcggagcg ccgcaggcgc gcagtcgaag
     1921 gacgcgtcgg cgttaacgcg cgacggtcgg gatgccgtcg ctcggacgtt cgtcgctcgc
     1981 gtccatgact cgctcctact cgtgcgcttt ttcgcaacac caaaggaata cgccgtcctg
     2041 gcttttgcga aaaagtcgca cgcctcgctc acggcatccc aaccgtcgcg ctttcgacga
     2101 gcagtccttc gactgcgctc gctcacgcga gctccgctca ggatgacaca agagctccgc
     2161 tcaggatgac acagcggctt cgctcagga
//
LOCUS       DASUCJ010000242         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1408498, whole genome shotgun sequence.
ACCESSION   DASUCJ010000242 DASUCJ010000000
VERSION     DASUCJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408498"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFU90_02990"
     CDS             <1..306
                     /locus_tag="VFU90_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478767.1"
                     /translation="SRLVSGIDWGSLLGSAAKIAASSLAMVAALHWIGTLGVTPEATL
                     ASRAWFLFGQIAIGGTVFVAVARMFNVEELELARRTIVAKFERNLVSPPDNREAPIA"
     gene            303..>2083
                     /locus_tag="VFU90_02995"
     CDS             303..>2083
                     /locus_tag="VFU90_02995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5478768.1"
                     /translation="MTLTATQERKPRFSVIIPAHNEQDRIGKTLEGYAGVFVDSEIIV
                     VLNGCTDGTRDVVNALRESHSNLIAIESPDAVGKGGAVRAGFLMAGADVVGYVDADGS
                     TPAEEMRRLCEAVGENDGVIGSRWLPSSRVAIRQPFGRRLASRNFNVIVRALFGLRYS
                     DTQCGAKVFRRSALERVMRDVETANLAFDVDLLYAMKRLKMRIREEPTYWIDMQGSRV
                     RLVPASLHMFAAVVRLRLHHSFLAVVVPMYDRLFPTNPVRTRHHLRILVINWRDPKHP
                     QAGGAETYLFEQAQRWTRWGHHVEWLSGGFSGGAPRDRIGSIPIRRVGNAFTVYAKVP
                     FTYLREFRDRFDVILDSSNGIPFFSPLFSMKPKICIVYHVHREVFKKHLPGWLAAMLA
                     WSEEKLVPLIYRRVHFVTISEDTRDEMRRVGIGNASAGLVRCGVDGRLVPGEKSSVPT
                     VLYLGRLKAYKRVDRLIEAFATVRGQLPAARLRIAGTGDALADLRALVERLGLQESVV
                     FEGFVDDDRKRELLQSAWVTASLSEMEGWGISAIEGNACGTPAIAYDVPGLREAIVHR
                     ESGLIVPEGGDAAAAILAVLQDGALRAR"
BASE COUNT          329 a          650 c          728 g          376 t
ORIGIN      
        1 agccggctgg tttcggggat cgattggggt tcgctgctcg gctcggccgc gaagatcgca
       61 gccagctcgc tcgcgatggt cgcggcgctg cactggatcg gaacgctggg cgttacgccc
      121 gaagcgacgc tggcgtcgcg cgcctggttc ctcttcggtc agatcgcgat cggcgggacc
      181 gtcttcgtcg cggtggcgcg gatgttcaac gtggaagaac tggagctggc gcgccgcacg
      241 atcgtcgcca agttcgaacg caatctcgtg agtccgccgg ataaccgcga ggcgccgatc
      301 gcatgacgct caccgctacg caggagcgca agccccgttt ctccgtcatc atcccggcgc
      361 acaacgagca agaccgcatc gggaagacgc tcgagggcta cgcgggcgtc ttcgtcgatt
      421 cggagatcat cgtcgtccta aacggatgca cggacggaac gcgcgacgtg gtgaacgcgc
      481 tgcgggaatc gcattcgaac ttgatcgcga tcgaaagtcc cgacgcggtg ggcaagggcg
      541 gcgcggtgcg cgccggattc ctgatggcgg gtgccgacgt cgtcggatac gtcgacgccg
      601 acggctcgac gccggccgaa gagatgcgcc gcctctgcga ggcggtcggc gagaacgacg
      661 gcgtcatcgg ttcgcgttgg ctgccgtcgt cccgggtcgc gattcgccag cctttcgggc
      721 gccggctggc cagccgcaac ttcaacgtga tcgtccgcgc gctgttcggc ctgcggtatt
      781 ccgacactca gtgcggggcc aaggtctttc gccgcagcgc gctggagcgc gtcatgcgcg
      841 acgtcgaaac ggccaacttg gcgttcgacg tcgacctgct gtacgcaatg aagcgcctca
      901 agatgcggat ccgcgaagaa ccgacgtact ggatcgacat gcagggttcg cgcgtccgct
      961 tggttccggc ctcgctgcac atgttcgcgg cggtcgtgcg ccttcggctg catcattcgt
     1021 ttttggcggt cgtcgtcccg atgtacgacc ggctgtttcc gaccaatccg gtgcgaacgc
     1081 gacaccacct tcgcattctg gtcatcaact ggcgagatcc gaaacacccg caggcgggcg
     1141 gcgccgaaac ctatctcttc gaacaagccc agcgctggac gcgctggggc caccacgtcg
     1201 agtggctcag cggcggcttt tcgggcggcg cgccgcgcga ccggatcggt tcgattccga
     1261 tccgccgcgt cgggaacgcg ttcaccgtct acgcgaaggt tccgttcacc tatttacgcg
     1321 aattccgcga ccggttcgac gtgattctcg attcgtccaa cggtattccg tttttctcgc
     1381 cgctgttctc gatgaagccg aagatctgca tcgtgtatca cgtgcatcgc gaggtcttca
     1441 aaaagcattt gcccggctgg ctcgcggcga tgctggcgtg gagcgaggaa aagctggtgc
     1501 cgctgatcta ccgcagggtg cacttcgtca cgatttcgga agacacgcgc gacgagatgc
     1561 ggcgcgtcgg gatcggcaac gcgagcgcgg gtttggtgcg ctgcggcgtc gacggacggc
     1621 tcgtgcccgg cgagaagtcg agcgttccga cggtgctcta tctcgggcgc ctcaaggcct
     1681 acaagcgggt cgaccggctg atcgaagcgt ttgcgacggt tcgcgggcag ctgcccgcgg
     1741 cgcggctgcg gattgccggc acgggcgacg cgctggcgga cttgcgcgcg ctggtggagc
     1801 gcctcggctt gcaagagtcg gtcgttttcg aaggcttcgt cgatgacgat cgcaagcgcg
     1861 aactcctgca aagcgcgtgg gtgaccgcga gcctttccga gatggaaggc tggggcatca
     1921 gcgcgatcga ggggaacgct tgcgggacgc cggcgatcgc gtacgacgta ccgggattgc
     1981 gcgaagcgat cgtccatcgg gagagcgggc tgatcgtgcc ggagggcggc gacgccgccg
     2041 cggcgatcct ggcggtgctg caagacggcg cgctgcgcgc gcg
//
LOCUS       DASUCJ010000243         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_736265, whole genome shotgun sequence.
ACCESSION   DASUCJ010000243 DASUCJ010000000
VERSION     DASUCJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736265"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VFU90_03000"
     CDS             complement(<1..1491)
                     /locus_tag="VFU90_03000"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5478769.1"
                     /translation="MTLVAHTGSIGLLLTAVCMTTAAPAQHAPPPFGNPAEWHWAPSR
                     SYHVLDYRLALHFDEPRGVVFGDEIVTLRPFGDAFRTFYLNSSGLTIDSVRLERANAA
                     EAPLAYTTDASRLWITLDHPSDAGTTLRVRVVYHGAPRTGLFFVNPTDAYPNAPREVF
                     SQGEPELNHYWFPCWDYPNDMATSETITTVPAGQVVVSNGRLVRVTHGAHTTTYDWVE
                     RVPHSSYLISLAVGPWRKISDRAGATPVAYYAPRSVDTATIRRSFRLTPDMIAFFGRA
                     TGVPYPYEQYAQTTVHDYLFGGQENVSATTLTDLTLHDARAEADYPSTALVAHELGQE
                     WFGDYVQGRDWADIWLNEGFATYMEALYTQHHDGNDAFRYEMVQDEELARAQDRRDYL
                     RPIVDRHYDDPLDMFDAITHEKGAAVLDMLRYVVDGSSAAAHPASQRETLFRAFHHYL
                     ALHHTHTATTSTLIDALRESTGLELGWFFHEWVVMAGTPSYRVSARY"
     gene            complement(1879..>2387)
                     /locus_tag="VFU90_03005"
     CDS             complement(1879..>2387)
                     /locus_tag="VFU90_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478770.1"
                     /translation="GIALFAALAAGLIAYFRVPRFRERVLQIPMPVLIAANIGRVLGV
                     FFLILYGVGRLPAPFAPSAGWGDVAVGLIALPLAAMAASGSVRPIWISLWNALGMLDL
                     ITAVALGVTSQPGAPLRVFSGSAEQVTTLPWVLIPTLLVPAYFLMHLLILRKLRAPRA
                     VSTAALAA"
BASE COUNT          412 a          779 c          828 g          368 t
ORIGIN      
        1 gtaccgcgcc gacacccgat agctcggcgt gccggccatg acgacccact cgtgaaagaa
       61 ccagcctaac tccagcccgg tggactcccg cagggcatcg atgagcgtgc tcgtcgtcgc
      121 cgtatgggtg tggtgcagcg ccaggtagtg gtggaaggcg cggaacagtg tttcgcgctg
      181 cgacgccggg tgagccgcgg cggacgatcc atccacgaca tagcgcagca tgtcgagcac
      241 ggcggcgccc ttctcgtgcg tgatcgcgtc gaacatgtct aacggatcgt cgtagtgccg
      301 gtcgacgatc gggcggagat agtcgcggcg gtcctgcgcc cgggccagct cttcgtcctg
      361 caccatctcg tagcggaagg catcgttgcc gtcgtggtgc tgcgtgtaca acgcctccat
      421 gtacgtggcg aatccctcgt tgagccagat gtcggcccag tcgcggccct gcacgtagtc
      481 gccgaaccat tcctgcccca gctcgtgcgc cacgagcgcc gtgctggggt agtcggcctc
      541 cgcgcgggcg tcgtgcagcg taaggtcggt gagcgtcgtc gcgctcacgt tctcctgtcc
      601 tccgaacagg tagtcgtgga ccgtcgtttg cgcgtactgc tcgtacggat acggcacacc
      661 cgtcgcccgg ccgaagaagg cgatcatgtc tggcgttagg cggaaggacc ggcggatggt
      721 ggccgtgtcg acgctccgcg gcgcgtagta cgcgaccggc gtcgcgcccg cgcggtcgct
      781 gatcttgcgc cacggcccca cggcgagcga gatcagatac gagctgtgcg gcacacgctc
      841 gacccagtcg tacgtggtcg tgtgtgcgcc atgcgtcacc cgcaccaggc ggccgttgga
      901 caccacgacc tgtcccgcgg ggacggtggt gatggtctcg ctcgtcgcca tgtcgttcgg
      961 gtagtcccag cacgggaacc agtagtggtt gagctcgggc tcgccctgcg agaacacttc
     1021 gcgcggcgcg ttcggatacg cgtcggtggg gttgacgaaa aagaggccgg tgcgcggcgc
     1081 gccgtggtag acgacgcgca cgcggagcgt ggtgccggca tccgatggat ggtcgagcgt
     1141 gatccagagg cgcgacgcgt cggtcgtgta cgccagcggc gcctcggcgg cgttggcgcg
     1201 ctcgaggcgc acggaatcta tcgttaggcc ggagctgttg agatagaacg tgcggaaggc
     1261 gtcgccgaat gggcggagcg tcacgatctc atcgccgaac accaccccgc gcggctcgtc
     1321 gaaatgcagc gcgagccggt agtcgagcac gtggtagctg cggctgggcg cccagtgcca
     1381 ctccgccgga ttgccgaatg gcggcggcgc atgctgggcc ggcgccgctg tcgtcatgca
     1441 gacggccgta agcaacaggc cgatgctgcc cgtgtgtgcg acgagtgtca tcggtacggg
     1501 ccgtcgctca tgtgagctcg cgccgcccga cccggcgttg ggcatctgga cggcgacggt
     1561 cgcgacgcga tagctgagtc cggcgagggc ggcgatgatg ccgacacggc catcgcatcg
     1621 cggagccagt gttgattttc acgttctcgt tctccgatcg gatcggacac tagtgcgcga
     1681 cactcgacaa cgcttcgtca gcggacgaac cgtggctgcc gcacgtcatc ctgctacgcc
     1741 cagaacgacg atggcaggcg tcttgtagaa tgtcaacaac gctgccgggc gcccatgcac
     1801 cagagcgccg cgcgcatcat ggagttgctg cctatcaagt aggcaaaaac tccatgatgc
     1861 gccgaacgca gcggtgatct acgcagccag cgcggccgtc gacacggccc gcggcgcgcg
     1921 aagcttgcgc aggatcagga ggtgcatgag gaagtacgcc ggcaccaaca acgtcggaat
     1981 cagcacccac ggcagcgtcg tcacctgctc ggccgacccg ctgaacacgc gcagcggtgc
     2041 gccaggctgg gaggtgacgc ctaacgcaac ggccgtgatc aggtcgagca tgccgagcgc
     2101 gttccacagc gagatccaga tcggacgcac ggatcccgac gcagccatcg cggcgagcgg
     2161 cagggcgatc aatccgaccg cgacatcgcc ccagcccgcc gacggcgcga acggcgccgg
     2221 cagtcggccc acgccgtaga ggatgagaaa gaagacgccc aacacgcgcc cgatgttcgc
     2281 ggcgatgagc acgggcatcg gaatctgaag cacgcgctcc cggaaccgcg gcacgcggaa
     2341 gtacgcgatc aatccggccg ccagcgccgc gaacagcgcg atcccga
//
LOCUS       DASUCJ010000244         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_384505, whole genome shotgun sequence.
ACCESSION   DASUCJ010000244 DASUCJ010000000
VERSION     DASUCJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384505"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..782)
                     /gene="aat"
                     /locus_tag="VFU90_03010"
     CDS             complement(72..782)
                     /gene="aat"
                     /locus_tag="VFU90_03010"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU5478771.1"
                     /translation="MIAWLRRGAAFPPLEQALAEPNGLLAAGQELTPERVIEAYRQGI
                     FPWYGVGQPVLWWSPDPRMVLYPDEFRCARSLARRIRQRQFEIRVDTAFDAVIGACAT
                     VPRNDDDRAETWITAAMQDTYRALHRLGYVHSVEAWREGELAGGLYGLALGRVFFGES
                     MFAHVTDASKVALAALVGLAQRQTVALIDCQQETRHLASLGARPIPRAHFAREIAQLI
                     HSTAAPGGWTPGPISEHA"
     gene            836..911
                     /locus_tag="VFU90_03015"
     tRNA            836..911
                     /locus_tag="VFU90_03015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:869..871,aa:Arg,seq:cct)
     gene            961..>1958
                     /locus_tag="VFU90_03020"
     CDS             961..>1958
                     /locus_tag="VFU90_03020"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478772.1"
                     /translation="MSLDTPSVHYRISPGNLRAHRFEVSCTVARPDRASQAFRLPTWI
                     PGSYLIREFARHVVAIGAERDGIAVPIVKEAKDLWRVAAGAGSVTVTAQVFAFDTSVR
                     GAYLDASRAFFNGACVFLCPEGLEAAPCTLDIVAPGDPAAADWRVATTLGRDEKTPGL
                     TAAEAERGFGRYRAANYDELIDHPVEMGAFVLATFDAGNVPHDIALSGVRHVAVDRLQ
                     ADLARICQWQIELFGGAGAKPPFDRYLFLVNATDHGHGGLEHRSSTSLLCSRTELPTR
                     GDANSTATGVADSPPAIDDAYLRFLGLASHEYFHSWNVKRIKPAEFVPYDLAHEAYT"
BASE COUNT          324 a          684 c          630 g          320 t
ORIGIN      
        1 caggtaactg cacgggtacg gcgccgtggc gtagaactgc agcgtcgcca gcggaaggtc
       61 gttcagcctg ctcacgcgtg ctccgatatc ggccccggcg tccagccgcc gggagccgca
      121 gtcgagtgta tcaactgcgc gatctcgcgt gcaaaatgcg cgcgaggaat cggtctcgca
      181 ccgagcgacg cgaggtgccg cgtctcctgc tgacagtcga tcaacgcaac cgtctgtcgt
      241 tgcgccaggc cgacgagcgc ggcgagcgca actttcgatg catcggtgac gtgggcgaac
      301 atcgattcgc cgaagaagac ccttcccaat gcaagaccgt agagtccgcc cgcgagctca
      361 ccttcccgcc acgcttcgac cgaatgcacg taaccgaggc gatgcaatgc gcggtaggtg
      421 tcctgcatcg ccgcagtgat ccacgtctcg gcccgatcgt cgtcgttgcg cgggacggtc
      481 gcgcaggccc cgatcaccgc gtcgaatgcg gtgtcgacgc gaatctcgaa ttgacgctga
      541 cggatgcggc gcgcgagcga acgagcgcaa cggaactcgt cgggatagag caccatccgc
      601 ggatccggac tccaccacag caccggctgg ccgacgccgt accacgggaa gatgccctga
      661 cggtacgcct cgatcacccg ttccggtgtc agttcctgac ccgctgcgag gaggccgttc
      721 ggctcggcga gtgcctgttc gagcggagga aaggcagcgc cgcggcgaag ccacgcgatc
      781 aacgccggat atccggcggc ggggctgcgc cgggaggata gaatcggcgg gcgccgcccc
      841 cgtagctcag tggatagagc acattcctcc taagaatggg gtcgcacgtt cgattcgtgc
      901 cgggggcgcc atgatgcgcg caaacgcggc gccctgtcat ccgccgcaga cgtcttcccg
      961 atgagcctcg acacccccag cgtccactat cggatctcgc ccggcaacct gcgtgcgcat
     1021 cgcttcgaag taagctgcac cgtcgcgcga cccgatcgcg cgagccaggc gttccgcctg
     1081 ccgacgtgga tccccggcag ctatctgatc cgcgagttcg cgcgccacgt ggtcgcgatt
     1141 ggcgccgagc gcgacggcat cgcggtgccg atcgtaaagg aggcgaagga tctctggcgc
     1201 gtcgccgctg gcgccggctc cgtcacggtg accgcacagg tcttcgcgtt cgacacttcg
     1261 gtgcgtggcg cctacctcga cgcgtcacgg gccttcttca atggcgcgtg cgtgtttctt
     1321 tgcccggagg gcctcgaagc cgccccgtgc acgctcgaca tcgttgcgcc gggcgatccc
     1381 gccgcagccg actggcgcgt cgcgacgacg ctcggtcgcg atgagaagac gcccggactc
     1441 actgccgcgg aagccgaacg tggcttcggg cgctaccgcg ccgcgaacta tgacgagctc
     1501 atcgatcatc cggtcgagat gggcgccttc gtgctcgcga ctttcgatgc cggcaacgtt
     1561 ccgcacgaca ttgcgctctc cggcgtgcgg cacgtcgccg tcgaccggct gcaggcggat
     1621 ctcgcacgca tctgccaatg gcagatcgag ctcttcggcg gtgccggtgc gaagccgcct
     1681 ttcgatcgct acctcttcct cgtcaacgcg acggatcacg gacacggcgg cctcgagcat
     1741 cgctcgtcga cgagccttct ctgctcgcgc accgagctcc ccacccgtgg cgacgcaaac
     1801 tcgacggcca ccggggtcgc cgattcgccg cccgcgatcg acgacgcgta tctgcgcttc
     1861 ctcggcctcg ccagtcacga gtatttccat agctggaacg tgaagcgcat caagccggcg
     1921 gagttcgtgc cctacgacct cgcgcacgag gcgtacac
//
LOCUS       DASUCJ010000245         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_858349, whole genome shotgun sequence.
ACCESSION   DASUCJ010000245 DASUCJ010000000
VERSION     DASUCJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858349"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFU90_03025"
     CDS             complement(<1..520)
                     /locus_tag="VFU90_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478773.1"
                     /translation="MYFDESHVRSKLAWDDLIATMESSLAAFSRGETLQPLRTVLTVT
                     EGKRFFGIMPAVAGDVMGLKAVTFYPNNHELGIPTHNGTILLFRTDTGEPLAAMDGRL
                     ITEMRTAAVSAAITKYLMPENARVLALLGSGVQAVAHLEALSSFHTFDEVRVWSRTPA
                     HARRFACERGAKE"
     gene            complement(549..1415)
                     /locus_tag="VFU90_03030"
     CDS             complement(549..1415)
                     /locus_tag="VFU90_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEU5478774.1"
                     /translation="MLQPDAGVLLYTANRDTPAVLAYRQKANGNVVPVVNISGSKTGL
                     NSPDALAMDSAGNVYVANDGANQVEVFAAGAHGNVAPSGLIGGSKSHLGPTEGLAIDA
                     SGNLYVSDYSDNAVTEYAAGARGNVAPIRTIAGSNTKLDTPVGMALDKKGRLFVANAA
                     GPSIVVFGPAAKGNAHPAFSIAGGNTGLSRPFALAFDSHQRLLVADENAGVLVFAKGA
                     KGNVAPLQKITGLSYPTGVTSDAADHIYAADFEGNSLDVYASNANGNATPLRKISGAK
                     TLLNNPCYLSLH"
     gene            1684..2136
                     /locus_tag="VFU90_03035"
     CDS             1684..2136
                     /locus_tag="VFU90_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5478775.1"
                     /translation="MKLPVAILASLLVASAVPVTVAIAATAPTIVTPSHLQWAPGKGM
                     PAGVKVAVLTGNPNASGAYAVRLMLPDGTLFGPHFHGDAEWVTVLQGTLMVGLGDKVD
                     KTKMKALGPGSFVAVPAGLHHYAMAKGATIIQLTGMGPMTMTAVKGSM"
     gene            complement(2158..>2789)
                     /locus_tag="VFU90_03040"
     CDS             complement(2158..>2789)
                     /locus_tag="VFU90_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478776.1"
                     /translation="VPQPRSRVAPQNPPRPVEPPQAARRPQPAARAAPPATRPHELAR
                     TAPTAEPQPSPEKIERNRPSTLEQQIAAQEQTFQQELARLRAANNPLSIAPKARETPS
                     TLHRTYFDVAGKRDLDAVQVQLIPIQRWTSGTMVCYYARYVAQYTHGGNEEGTVPWPV
                     CYPAGDDKIAYPPYVHDIPIPIPQPDYVLPPGTYLTPLLARIYGQRRIR"
BASE COUNT          476 a          797 c          989 g          527 t
ORIGIN      
        1 tttccttggc gccgcgttcg caggcgaagc gtcgcgcgtg cgcgggcgtg cggctccaga
       61 cgcggacttc gtcgaaggtg tgaaagctgg aaagcgcttc caggtgggca actgcctgca
      121 cgccgctgcc cagcagcgcc agcacgcgcg cgttttcggg catgaggtat ttcgtgatgg
      181 cggccgaaac ggcggcggtg cgcatctcgg tgatcaagcg cccgtccatc gcggcgagcg
      241 gctcgccggt gtcggtgcga aagagcagga tcgtgccgtt gtgcgtcggg atgccgagct
      301 cgtgattgtt aggatagaac gtcaccgctt tgagccccat cacgtcgccg gcgacggcgg
      361 gcatgattcc gaaaaaacgc ttgccttcgg taacggtcaa tacggtgcgc agcggttgca
      421 gggtttcgcc gcgggaaaac gcggcgaggg acgattccat cgttgcgatg agatcgtccc
      481 acgcgagttt gctgcgaacg tgcgattcgt cgaagtacac ggtcgcggtt tacgtaaagg
      541 cccctatgct agtggagaga caaatagcag ggattgttga gcagcgtctt cgcgccggag
      601 attttccgca gcggcgtcgc gttgccgttg gcgttgctcg cgtaaacgtc gaggctgttg
      661 ccttcgaaat cggcggcata gatgtgatcg gccgcatccg acgtgactcc ggttgggtac
      721 gagaggcccg tgatcttctg cagcggcgcg acgttgcctt tggcgccctt ggcgaagacg
      781 agcacgccgg cgttttcgtc ggcgacgaga aggcgctgat gcgaatcgaa cgcaagggcg
      841 aacgggcggc tcaaaccggt gttgccgccg gcgatcgaaa acgccgggtg agcgttgccc
      901 ttggcggccg gaccgaagac gacgatcgac gggccggcgg cgtttgcaac gaacaggcgt
      961 cccttcttgt cgagcgccat gcctacgggc gtgtcgagct tggtgttgct gccggcgata
     1021 gtgcgaatcg gtgcgacatt gccgcgcgcg ccggccgcat attccgttac ggcgttatct
     1081 gagtagtcag acacatagag attgccggag gcatcgatcg ccaagccttc ggtagggccg
     1141 aggtgcgact tcgatccgcc aatcaatccc gagggtgcga cgttgccgtg ggcgccggcg
     1201 gcgaacactt cgacttgatt cgcgccgtcg ttggcgacgt aaacgtttcc agcggaatcc
     1261 atcgcgagcg cgtcgggaga attgagtccg gttttcgagc cgctgatgtt tacgaccgga
     1321 acgacgttgc cgttggcctt ctgacggtag gcgagaacgg cgggcgtgtc gcgattggcg
     1381 gtatacaaca gcacgccggc gtcgggctgc agcatcggtg aaacgcccgc tcccggtccg
     1441 gatgcggttc cgggcaagtt tgcggagccg ccgcacgcgg tcaataggaa agcaaagcca
     1501 attgccacaa cccgacgcat agaaaaatac ctcctcagca acgacggttg cgactgactt
     1561 cgcgccggtt tcgagcgcgc ctgcggacgc gcgccgggcc acgcgcaagg gtagtccaca
     1621 tggactaccg tagctcgacc gtagcgggcg aattcgttac catagcatta aggagggttc
     1681 tgaatgaaac tacccgtcgc tattcttgcc tcgctgctcg tagcgagcgc agttcccgtt
     1741 accgtcgcta tcgccgcaac ggcaccgacg atcgtgacgc cgtcgcacct gcagtgggcg
     1801 cccggtaaag gaatgccggc cggcgtcaaa gttgcggtgc tgacggggaa tcccaacgct
     1861 tccggagcgt acgcggtgcg cttgatgttg cccgacggga cgctcttcgg tccgcatttc
     1921 cacggcgacg ccgagtgggt gacggtgttg caaggaacgc tgatggtcgg ccttggcgac
     1981 aaagtggata aaacgaaaat gaaggcgctc ggccccggat cgttcgtcgc ggtgcccgcc
     2041 ggtctgcacc actacgcgat ggcgaaaggt gcgacgataa tccagctaac cggaatgggg
     2101 ccgatgacga tgaccgcggt gaaaggcagc atgtaggcaa ttgcctttac atagcggtta
     2161 gcggatgcgc cgctggccgt aaatgcgtgc gagcagcggc gtgaggtagg tgccgggagg
     2221 cagcacgtaa tcgggttgag ggatcggtat cgggatatcg tgcacgtacg gcgggtaggc
     2281 gattttgtcg tcgcccgccg gatagcacac cggccacggc accgttccct cttcgtttcc
     2341 gccgtgggtg tactgcgcga cgtagcgcgc gtagtagcac accatcgttc cgctggtcca
     2401 acgctggatc ggaatcagct gcacttggac ggcgtcgaga tcgcgcttgc cggcgacatc
     2461 gaaataggtg cggtgcagcg tcgacggcgt ttcgcgcgct ttcggcgcga tgctgagcgg
     2521 attgttggcc gcgcgcagcc gcgcaagttc ttgctggaac gtttgttctt gcgcggcgat
     2581 ctgctgctcg agcgtgctcg ggcgattgcg ttcgattttt tcgggcgacg gttgaggttc
     2641 ggcggtgggc gccgtgcgcg cgagctcgtg cgggcgagtg gccggcggcg ccgcgcgggc
     2701 ggcgggctgc ggacggcgcg cggcttgcgg cggctcgacc ggacgcggcg ggttttgcgg
     2761 ggcgacgcgc gagcggggct gggggaccg
//
LOCUS       DASUCJ010000246         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_338753, whole genome shotgun sequence.
ACCESSION   DASUCJ010000246 DASUCJ010000000
VERSION     DASUCJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338753"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFU90_03045"
     CDS             complement(<1..843)
                     /locus_tag="VFU90_03045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5478777.1"
                     /translation="MTLDGVNETPVWTAGNAGTAAPEPAALPSRADVAIVGAGYTGLS
                     AALALAKRGRSVAVLESGTVGFGASSRNGGMALTGLKLAPDVLLRRYGAATAREMFSA
                     SLEAIDLVESLIASERIECAFERCGHLELASKAAHFVAFGRTAAALSEHFGHAVRLLG
                     RAELAGEIGSTAFYGGIVDPRSAALDPLRYVMGLAHAARRSGATICERTPVEAVEPVD
                     GQWRLRSSRGELTARNVLLATGAYTGRAFPALRRRLVPIGSYAIASEPLPSSLADAVL
                     PRKRA"
     gene            complement(848..>1543)
                     /locus_tag="VFU90_03050"
     CDS             complement(848..>1543)
                     /locus_tag="VFU90_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478778.1"
                     /translation="VRVPNPYYCTKVTPACPYALQPLFDVNGSYAPYDVIPSPFNAAN
                     GFDVPDGLSFVVNYRHGRYAITPSLRYSSGSSYGSPLVWPGYVPQACTQNPALTPLTP
                     GATCGSGGAIFLPDPYNGNHFDGLGFYQQPSQLALNVSATYDFSDRLSVDVSAVNVYN
                     KCYQRGYAWDNPITCVYSTLPSNILAPAGNFLKAPPVQLRYPYGTFFNITEVGNTSVL
                     QPFGFFVNFNVKI"
BASE COUNT          287 a          500 c          517 g          239 t
ORIGIN      
        1 agcccgcttt cgcggaagca ccgcatcggc cagggacgac ggaagcggct cggacgcgat
       61 tgcgtacgaa ccgatcggga cgagacgccg ccgcagagct ggaaaagctc tccccgtgta
      121 ggcaccggtc gcgagcagca cgtttcgcgc ggtgagttcg ccgcgggacg acctcaaccg
      181 ccattgcccg tccaccggtt cgacggcctc gaccggcgtg cgttcgcaaa tcgtcgcgcc
      241 ggaacggcgt gccgcgtgcg cgagccccat gacgtaacgc agcgggtcga gtgccgcgct
      301 gcgcgggtcc acgataccgc cgtaaaacgc cgtcgaaccg atttcgccgg cgagctcggc
      361 gcgcccgaga agccggaccg catgcccgaa gtgctcggac agcgcggcgg ccgtacggcc
      421 gaaggcaacg aaatgcgccg ccttgctcgc gagctccaag tgcccgcacc gttcgaaagc
      481 gcactcgatt cgttcgctcg cgatcaggct ctcgacgagg tcgatcgctt ccagcgatgc
      541 ggagaacatc tcccgtgccg tcgcagcacc gtagcggcga agcagaacgt cgggcgcgag
      601 tttcaatccg gtgagcgcca tcccgccgtt gcgcgagctg gcgccgaaac cgacggtgcc
      661 gctctcgagg accgcgaccg aacggccccg cttggctagt gcgagcgctg ccgagaggcc
      721 ggtgtagccc gcgcccacga tcgccacgtc tgcgcgtgac gggagcgcgg ccggctcggg
      781 cgccgcggtt ccggcgttcc ccgccgtcca caccggcgtt tcgttcacgc cgtccagcgt
      841 cacgccacta gatctttacg ttgaagttta cgaaaaaccc aaacggctga agtaccgacg
      901 tattgccgac ctcggtaata ttgaagaacg tcccataggg gtagcgcagc tggacgggcg
      961 gtgctttgag gaagttgccc gccggcgcca agatgttcga cggaagcgtg gagtaaacgc
     1021 acgttatcgg gttgtcccaa gcgtagccgc gctgatagca cttattgtaa acgttcaccg
     1081 ccgagacgtc gaccgacagg cgatcgctaa aatcgtaggt cgcgctcacg ttaagcgcca
     1141 gttgcgaggg ctgctggtag aagcccaggc cgtcgaagtg attgccgtta taggggtccg
     1201 gcaagaagat ggcgccgccc gagccgcacg tcgctcccgg cgtcaagggc gtcaaggccg
     1261 gattttgcgt gcaggcctgg gggacgtaac ccggccacac cagcggcgat ccatagctcg
     1321 agccgctgct atagcgcaac gacggcgtaa tcgcgtaacg gccgtggcga tagttcacga
     1381 cgaacgagag cccgtccggc acgtcgaagc cgttggccgc gttgaagggt gaggggatga
     1441 cgtcgtacgg cgcgtacgag ccgtttacgt cgaacagcgg ctgcagcgcg tacggacacg
     1501 ccggcgtcac cttcgtgcaa tagtaggggt tgggcacgcg cac
//
LOCUS       DASUCJ010000247         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1347654, whole genome shotgun sequence.
ACCESSION   DASUCJ010000247 DASUCJ010000000
VERSION     DASUCJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347654"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFU90_03055"
     CDS             complement(<1..837)
                     /locus_tag="VFU90_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478779.1"
                     /translation="MRMQRTLLGRILVLGLASLQATACDALVGLGPPLRPSAGDAVGA
                     DGAASPALEDDANRLGLVRINGTNTGVLLAPDLVITCGHCVHYGPYENGANSVVLPGP
                     VAGASNSDQVYWFGLGAGNNPDLALLHLSPPLAGVQTSGIEVSLDGVPGAQRIGASLV
                     GYEWADAGANLWWTGRARAVAASRYELAVEPPSAPWQDDPGAPYFIDPGDGGTLLVAL
                     QTDGPADAGAARTAVALGDMGPWIAAVRVMDWNAGLSAERVPVAWDEVATSYWSFTDA
                     AAV"
     gene            937..>1548
                     /locus_tag="VFU90_03060"
     CDS             937..>1548
                     /locus_tag="VFU90_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5478780.1"
                     /translation="MAGTHDEATAQPGASSSGELDFAPYLVLVLEGDRPLAGSRRHSL
                     EGIDVVHIGRGGARESSRTHGRLDVRVPSRFMSEEHARLVRVGSAWAVEDTASRNGCF
                     VNGKQVTRAVLSAGDRLEVGRTFFRVTHAPRMPESSAGGVFGESVETPTTFVPHFAAR
                     LSALAAVARSPVSVLIFGESGTGKEVLARRSHEASGRRGDFVAV"
BASE COUNT          253 a          537 c          523 g          235 t
ORIGIN      
        1 gaccgcggcg gcgtccgtga acgaccagta gctcgtcgcg acttcgtccc acgcgacggg
       61 cacgcgctcc gccgagagcc ccgcgttcca gtccatgaca cgaacggcgg caatccaggg
      121 gcccatgtcg ccgagcgcga cggcggtgcg cgccgccccg gcgtccgcgg gcccgtcggt
      181 ctgaagagcc acgagcaggg taccgccgtc accgggatcg atgaaatacg gcgctcccgg
      241 atcgtcctgc caaggtgccg atgggggttc taccgcgagc tcgtaacgtg acgcggccac
      301 ggcgcgcgcc ctgccggtcc accagagatt ggcccccgca tcggcccact cgtaaccgac
      361 gagcgatgcg ccgatgcgtt gcgcccctgg aaccccatcg agcgacacct cgataccgct
      421 cgtctgcacg ccggcgagcg gcggcgacaa atggagcaac gcgagatcgg ggttgttccc
      481 cgcgcccaag ccgaaccagt aaacctgatc cgaattcgat gcgcctgcga cggggccggg
      541 caggacgacg gaattcgctc cgttctcgta aggaccataa tgcacgcagt gaccgcaagt
      601 gatcacgagg tcgggcgcaa gcaagacgcc cgtgttcgtc ccgttgatcc gaacgagacc
      661 caatctgttc gcgtcatcct ccagcgccgg cgacgccgcc ccatcggcac cgacggcatc
      721 gcccgccgat gggcgcaaag ggggcccgag gccaacgagc gcgtcgcagg ccgtcgcttg
      781 gagagaagcc aatcccagga cgaggatgcg tccgagcagc gtccgctgca tgcgcatgcg
      841 ccgccagcga aactctcttc agcgcgggct gtcaaccccc gaagggcgcg gacggggcgg
      901 ttgatcgcat cgcgcacggc agtatttcat gtcgtgatgg cgggaacgca cgacgaagcc
      961 acggcccagc ccggcgcatc ctcttcggga gagctcgatt ttgcgccgta cctggtgctc
     1021 gttctggagg gcgatcgacc actcgccggc agtcgtcgcc atagcctcga gggcatcgac
     1081 gtcgtgcaca tcggccgggg tggcgcgcgc gaaagttcgc gcacccatgg gcgactcgat
     1141 gtccgtgttc ccagccgctt catgtcggag gagcacgcgc gattggtccg tgtgggttcg
     1201 gcgtgggctg tggaggacac cgcgagccgc aacggctgct tcgtcaacgg caagcaggtc
     1261 acgcgcgctg tactctccgc cggtgatcgg ctcgaagtcg ggcgcacgtt ctttcgggtc
     1321 acgcacgctc cccgaatgcc ggagtcctcg gcgggcggtg tctttggcga atccgtcgag
     1381 acgccgacga cgttcgtgcc tcacttcgcg gcgcggctct ccgcgctcgc cgccgtggcg
     1441 cgctcgcccg tgtccgtcct gatcttcggc gaatccggca cgggcaaaga agtgctcgcg
     1501 cggcgaagcc acgaggcgag cggacggcgc ggtgatttcg tcgccgtc
//
LOCUS       DASUCJ010000248         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_461180, whole genome shotgun sequence.
ACCESSION   DASUCJ010000248 DASUCJ010000000
VERSION     DASUCJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461180"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFU90_03065"
     CDS             <1..364
                     /locus_tag="VFU90_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478781.1"
                     /translation="TAQEGSYAVETGNDYADLSWFGSPLIAWVRDHGTGRELFTNYPT
                     ALYFHANRFSRGLPQAPRPDTARAFAETVAEAHGLIVAFNRASRFAASPTALMQLVPP
                     PVHVVFRTHDGAIYELPR"
     gene            complement(382..>1084)
                     /locus_tag="VFU90_03070"
     CDS             complement(382..>1084)
                     /locus_tag="VFU90_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478782.1"
                     /translation="LSIAASAHAQDSSSTPTTPPSQPATSISPAARHDTTRAADTLAA
                     DSALTRAEERARSRANRTFRIDMALGFVTSILAHESGHIIAALAVGGHPSFGFNKFRP
                     TIYSGIDASLHPTKQFIFSSAGLTVQSVLDEAILDVPHRGGSAFERGLLAGGIGTALF
                     YITLGRNGAVSDVAYMAQSSKLSKDQVSLIYGGIAVLHAVRIHGKAKYAHFFTAPSDR
                     GGVKVGMRIDGDWGH"
BASE COUNT          187 a          349 c          363 g          185 t
ORIGIN      
        1 taccgcacag gaagggtcgt acgccgtcga aaccggaaac gactacgccg atctgagttg
       61 gttcggctcg ccgctcatcg cctgggtgcg cgaccacggc accggccgcg aactcttcac
      121 caactatccg acggcgttgt acttccatgc gaaccggttc tcgcgcgggc tcccgcaagc
      181 gccgcgtcct gacaccgcgc gcgcattcgc cgagaccgtc gccgaagcgc acggtctcat
      241 cgtggcgttc aaccgcgcga gccggttcgc cgcgtcaccc accgcgttga tgcagctcgt
      301 tccaccgccg gtgcacgtcg tgtttcgcac acacgatggc gcgatctacg aactgccgcg
      361 ctgacgcgct cgaccgtgtg gtcagtggcc ccagtcgccg tcgatccgca tgccgacttt
      421 caccccgcca cgatcgctcg gcgccgtaaa gaagtgggcg tacttcgcct tgccatgaat
      481 tcgcaccgcg tgcaataccg cgataccgcc ataaatcaga gacacctgat ctttggacaa
      541 cttcgagctc tgcgccatgt aggcgacgtc gctcaccgcg ccgtttcggc cgagggtgat
      601 gtagaacagc gcggtgccaa tgccgccggc caacaggccg cgctcgaagg cggagccgcc
      661 gcggtgcggc acatcgagga tcgcttcgtc gagcacggac tgaaccgtta gaccagcgct
      721 cgagaaaatg aactgcttcg tggggtggag gctcgcgtcg atgccggaat agatcgttgg
      781 acgaaacttg ttgaagccga aggacgggtg tccgccaacg gcgagcgcgg caatgatgtg
      841 cccggactcg tgcgcgagaa tgctcgtgac gaagccgagc gccatgtcta tgcggaacgt
      901 gcggttcgcg cgtgagcgtg cacgctcctc cgcacgggtg agggccgagt cggcggcaag
      961 ggtgtcggct gcacgcgtcg tgtcgtggcg cgcggcgggt gaaatgctgg tcgccggctg
     1021 cgagggcggc gtcgttggag tggatgacga gtcctgggcg tgcgcgctcg cggcgatcga
     1081 gaga
//
LOCUS       DASUCJ010000249         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1500455, whole genome shotgun sequence.
ACCESSION   DASUCJ010000249 DASUCJ010000000
VERSION     DASUCJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500455"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /locus_tag="VFU90_03075"
     CDS             <1..>1117
                     /locus_tag="VFU90_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEU5478783.1"
                     /translation="LETNAADELIIAIPSATREQIQAIVNRCLSTRLPFKILPAIAAL
                     SGRVQMSQVRPVRVEDLLGRDPVDLDSAVVRGAIAGRRVLITGAGGSIGAELARQIAT
                     FDPASLTLIERSENALFLIEQELHRKHPQLDVRPAICDICESSDVARLFREARPEIVY
                     HAAAFKHVPMMEAHVCHAVQNNVFGTRNIAEAAAQYGATFVLISTDKAVSPTNIMGAT
                     KRLAEQLVLSPADSRAHHFVVVRFGNVLGSNGSVVPLFSEQIADGGPVTVTHPEATRY
                     FMTIPEAVQLVLQASALEEARDRIVMLEMGHPIKIVDLARNLIRLSGLEPDADIPIVF
                     TGLRPGEKLHEQLTSETEETMPTRFEKIRIVKTGASRS"
BASE COUNT          190 a          349 c          395 g          183 t
ORIGIN      
        1 gctggagacc aacgcagccg acgagctgat catcgcgatc ccgtcggcga cgcgggagca
       61 gattcaggcg atcgtgaatc gctgtttgtc cacccggctg ccgttcaaga ttctgccggc
      121 gattgccgcg ctgtcgggcc gcgtgcagat gagtcaggtg cgaccggtgc gcgtggaaga
      181 cctgttgggc cgggatccgg tggacctcga ttcggcggtc gtgcgcggcg ccatcgcggg
      241 acggcgggtg ctgatcaccg gggccggcgg gtcgatcggc gccgagctgg cgcggcagat
      301 cgcgacgttc gatccggcgt cgctcacgct gatcgagcgg tcggagaacg cgctgttcct
      361 catcgagcag gagctgcacc gcaagcatcc gcagctcgac gtgcgcccgg ccatctgcga
      421 catctgcgag agctccgacg tggcgcggct gttccgtgag gcgcgtccgg agatcgtgta
      481 tcacgccgcg gcgttcaagc acgtcccgat gatggaagcg cacgtctgcc acgcggtgca
      541 gaacaacgtc ttcggcacac gcaacatcgc cgaggcggcg gcgcagtacg gtgcgacgtt
      601 cgtgctgatt tcgacggaca aggccgtgag cccgacgaac atcatgggcg ccacgaagcg
      661 gctcgccgag cagctggtgt tgtcgccggc cgattcacgc gcccaccatt tcgtggtggt
      721 ccggttcggc aacgtgttag gcagcaacgg cagcgtcgtg ccgctgttca gcgagcagat
      781 cgccgatggc ggccccgtga cggtgacgca tccggaagcg acgcgctact tcatgaccat
      841 ccccgaggcg gtgcagctcg tgttgcaggc gtcggcgctg gaggaagcgc gcgaccggat
      901 cgtgatgctt gagatgggcc atccgatcaa gatcgtcgat ctggcgcgga acctcattcg
      961 gctgtcggga ctcgaacccg acgcggacat cccgatcgtc ttcacggggc tccggccggg
     1021 cgagaagctg cacgagcagc tgacgtcgga aaccgaagag acgatgccta cacgcttcga
     1081 gaaaatccgg atcgtgaaga cgggcgcctc gcggtcg
//
LOCUS       DASUCJ010000250         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_338829, whole genome shotgun sequence.
ACCESSION   DASUCJ010000250 DASUCJ010000000
VERSION     DASUCJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338829"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFU90_03080"
     CDS             complement(<1..190)
                     /locus_tag="VFU90_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478784.1"
                     /translation="MEVSLSSRYHLLRTLACGAIGWLAAPALASAAAPPPIPKLTPDA
                     VTHHALVLDGRTLTYAARA"
     gene            complement(270..572)
                     /locus_tag="VFU90_03085"
     CDS             complement(270..572)
                     /locus_tag="VFU90_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478785.1"
                     /translation="MPFLAVAHLRDARCPECDFTLAVPGARSFIVSPDGYPVLFDPGD
                     PPAEMTVELACPNGHALTLLLPNDVAAEETSTIPERAPIGRDALLRSGTTESGRSL"
     gene            complement(584..2386)
                     /locus_tag="VFU90_03090"
     CDS             complement(584..2386)
                     /locus_tag="VFU90_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5478786.1"
                     /translation="MTPEPPGKETLPQLIRRALASPRAEVLKERVDGSWAEITSARLL
                     ERVENLACAMRAAGLAAGDRVALIAHDCVDWIVADFAALFAGCVVVPIYPTQALDYTA
                     YILEHSAARLIFVDSRATLERIAAAGAALPRAIVFDSNGDDGLAAFEEGGATARAGRP
                     ELPGAYEAALHPDDLAVLIYTSGTTGPPKGVMLSHDNLGFDAQAALRYGLAELVEEST
                     VLSVLPYSHIFEHTMVYVYLIARLRCYVCHDPNALLPDMLDARPSVMTAVPRIFDRVL
                     AGVKGAAMRAGGLQAALVPWALDIGRKHARAQTFGGGAPAWLALQYAVARAVVLRKVR
                     AKLGLDRLRFFVSGSAALHIDTAMTFLGLELPIMQGYGLTETSPVISVSLRTANEYGA
                     SGRPIPGVEVSIAADGEILTRGRHVMLGYYRDPEATAATIVGGWLHTGDIGEIDERGF
                     LRITDRKREVFKTDTGKWISPARVEGSIKRSVFVAQVMVVGNGRPHPAALVCPNWDLV
                     RLELSLPADAPPERLAERDDVREFMTRVVERQTRELATFEQIRRVIVVPREFSVEAGE
                     LSPTMKIKRRVVEQRHADAIDRAYREDLHAHTHV"
     gene            complement(2440..3072)
                     /locus_tag="VFU90_03095"
     CDS             complement(2440..3072)
                     /locus_tag="VFU90_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5478787.1"
                     /translation="MSTRVAVALVALVLAAAALWHPAPRPVLQAISAPSPAWQPAAAR
                     RRTGEFRTPPEGAVVYVAGAVRKPGLYRLRPGDRVADAIGRAGGTLVPAGSGSVNLAA
                     RTADGDEIYVPEPGEPAPRAPGCAGNRRRSVRSHRSDAAPADLGSVDLNAADAPTLAR
                     VPGIGRAIAARIVEMRRLDGAFASSDELLDVAGMTQARLDRARPFLRQQP"
     gene            3137..>4476
                     /gene="leuS"
                     /locus_tag="VFU90_03100"
     CDS             3137..>4476
                     /gene="leuS"
                     /locus_tag="VFU90_03100"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5478788.1"
                     /translation="MHSMAEAYDFKSVERKWRDRWEGEDLYRAVDDDPRPKYYVLEML
                     PYPSGDLHVGHAKNYSLGDAVARMMRMLGYNVLHPMGWDAFGLPAENAAIAHGADPAS
                     WTTQNIANMKSQLRLIGTSYDWTREVATCDPEYYRWNQWLFLRLYEAGLAYKREAPVN
                     WCPHDRTVLANEQVVNGRCWRCETPVVRRNLSQWFLKITDYADRLLTDLDKLDGWPER
                     TRTMQRNWIGRSEGVQLAFAVDGLDAAIEVFTTRVDTLYGATFLAVAPEHPLVSRLGE
                     VVTREEAAAVAAFAERVSAQSELERTSLLEKQGLFTGAYAINPLSHERIPIWVTNYVL
                     ADYGTGAVMGVPAHDERDFDFAHTHGLPVACVVVPAGELEDGTLPQLPFVEDGRLIAS
                     GDFSTMSSARARETIGARLLALGVGKPAVTTRLRDWLVSRQRYWGTPIPIVYCE"
BASE COUNT          774 a         1502 c         1512 g          688 t
ORIGIN      
        1 ccgcgcgcgc cgcataggtc agcgtgcgtc cgtcgagcac cagcgcgtga tgcgtgacgg
       61 cgtcgggcgt cagcttcgga atcggcggag gtgccgccgc cgaggcgagc gccggtgcgg
      121 caagccaccc gatcgcgccg caagcaagcg tgcgcaatag atgataacgc gaactcaaag
      181 agacctccac gtggacggat gcgccttctg attatcgcaa ttcgaacccg gaccttcacc
      241 ggatccgcgc gcgcggaatc aaagttccat caaagggacc gtcccgattc ggtcgtcccc
      301 gagcgcagca gcgcgtcgcg accgatcggc gctcgctcgg gaatcgtact cgtttcctcg
      361 gcggcaacgt cgttgggcag gagcagcgtc agcgcgtgcc cgttggggca ggcgagctcg
      421 accgtcatct cggccggcgg atcgccggga tcgaaaagca ccgggtatcc gtcggggctc
      481 acgatgaagg agcgcgcccc cggcaccgcc agcgtgaagt cgcactccgg gcagcgggcg
      541 tcgcgcaaat gcgcgaccgc aagaaacggc atcggaccgg cctttatacg tgcgtgtggg
      601 cgtgaaggtc ctcgcgatac gcgcgatcga tcgcgtcggc gtggcgctgc tcgacgaccc
      661 gccgcttgat cttcatcgtc ggcgagagct cgccggcctc gacgctgaac tcgcgaggga
      721 cgacgatcac tcgccgaatt tgttcgaagg tcgccagctc gcgcgtctgg cgctcgacca
      781 cgcgcgtcat gaactcgcga acgtcgtcgc gttcggccag ccgttcggga ggtgcgtccg
      841 ccggaagcga aagctcgagg cgcacgagat cccagttagg acaaacgagt gccgcgggat
      901 gcggacgtcc gtttccgacg accatcactt gcgcgacgaa aaccgatcgc ttgatcgatc
      961 cttccacgcg agcgggagag atccactttc cggtgtcggt cttgaaaact tcacgtttgc
     1021 ggtcggtaat gcgcaagaag ccgcgttcgt cgatctcgcc gatgtcgccg gtatgcagcc
     1081 atccgccgac gatcgtcgca gcggttgcct cggggtcgcg gtagtagccg agcatcacgt
     1141 gccggccgcg cgtgagtatc tctccatcgg cggcgatcga cacctcgacg cccggaatcg
     1201 gccgccccga cgcgccgtat tcgttagcgg tccggagact cacgctgatc accggcgagg
     1261 tttcggtgag cccgtatcct tgcatgatgg gaagctccag gccgaggaac gtcatcgcgg
     1321 tgtctatgtg cagtgccgcg ctgccgctga cgaagaaccg cagccgatcg aggccgagtt
     1381 tcgcgcgtac cttgcgcagc acgacggcgc gcgcgacggc gtactgcagg gcgagccacg
     1441 cgggcgcgcc gccgccgaac gtttgcgcgc gtgcatgctt gcgcccgata tcgagcgccc
     1501 agggaacgag cgcggcctga aggcctccgg cccgcatcgc ggcgcccttg acgccggcca
     1561 gcacgcggtc gaagattcgc ggcaccgccg tcatgaccga cggacgggcg tcgagcatgt
     1621 cgggcagaag cgcgttcgga tcgtggcaca cgtagcagcg cagacgcgcg atcaggtaaa
     1681 cgtacaccat cgtgtgctcg aagatatgcg agtagggaag cacggagagg acggtgctct
     1741 cttcgacgag ctcggccagc ccgtaccgca gcgcggcttg cgcatcgaat ccaaggttgt
     1801 cgtgcgacag catcacgccc ttgggagggc cggtggtgcc cgaggtgtag atcagcaccg
     1861 ccaaatcatc gggatgcagc gccgcctcgt acgcgcccgg cagttcggga cggccggcgc
     1921 gggcggtcgc gccgccctct tcgaacgccg ccaacccatc gtccccgttc gaatcgaaca
     1981 cgatcgcgcg cggaagcgcc gcgccggcgg ccgcgatgcg ctcgagggtc gcccggctat
     2041 cgacgaatat cagccgcgca gccgaatgct ccaagatata cgcggtatag tcgagcgctt
     2101 gcgtcgggta aatcggtacg acgacgcacc ccgcgaacaa agccgcgaag tcggcgacga
     2161 tccagtcgac gcaatcgtgc gcgatgagcg ccacgcgatc gcccgccgcg agtccggcgg
     2221 cgcgcatcgc gcaagcgagg ttttcgactc gttcgagcaa tcgcgccgag gtaatttctg
     2281 cccacgagcc gtcgacgcgc tccttgagaa cctccgcacg cggcgatgcg agcgctcggc
     2341 ggatcagctg cggaagcgtt tccttcccgg gcggctccgg cgtcattcgg ccgctttcgc
     2401 cccgctcaaa cgccgctccg ctggcggacc tagggaaccc tacggctgct gacgcaaaaa
     2461 cggccgcgcg cggtccaagc gcgcttgcgt cattccggca acgtcgagca gctcgtccga
     2521 ggaagcaaaa gcgccgtcga ggcgccgcat ctcgacgatc cgcgcggcga tcgcgcgacc
     2581 gatgccgggc acgcgcgcca gcgtcggcgc gtcggcggcg ttgaggtcga cgcttccaag
     2641 atcggccggc gcggcatcgc tgcggtgcga acgcaccgag cggcgccgat tgccggcgca
     2701 gcccggcgcg cgcggtgccg gttcgcccgg ctccggcacg tagatttcgt cgccgtcggc
     2761 cgttcgcgcg gcgagattga cggaacccga gccggccggg acgagcgtgc cgcccgcgcg
     2821 cccgatcgcg tcggctactc gatcgccggg acgaaggcga tacaaccccg gttttcgcac
     2881 ggcgcccgcg acgtaaacga ccgcgccctc cggcggcgtg cggaattctc cggtgcgccg
     2941 ccgtgcggcg gccggctgcc acgccggcga cggtgcggag atcgcttgca gcaccggccg
     3001 cggggccgga tgccagagtg cggcggccgc aagaaccaac gctaccaagg ctaccgcgac
     3061 gcgcgtggac atgccgtccc ttcacgcgcg cgcgtccgac cggaaaggta gcggtgcggc
     3121 gcgggaagcg tgtaccatgc attctatggc cgaggcatac gatttcaagt ccgtcgagcg
     3181 caagtggcgc gatcgctggg agggcgaaga cctctatcgc gccgtcgacg acgatccgcg
     3241 gcccaagtat tacgtcctgg aaatgctgcc ttacccatcg ggcgatcttc acgtcggcca
     3301 cgccaagaac tattcgctcg gcgacgcggt cgcacgcatg atgcggatgc tcggctataa
     3361 cgtgctccat ccgatgggtt gggacgcgtt cggacttccc gccgagaacg ccgccatcgc
     3421 gcacggcgcc gatccggcgt cgtggacgac gcagaacatc gccaacatga aaagccagct
     3481 gcggctgatc ggcacgagct acgattggac gcgcgaggtc gccacctgcg atcccgaata
     3541 ctaccgctgg aaccagtggc tgtttctgcg gctctacgaa gcggggctcg cgtacaagcg
     3601 cgaagcgccc gtgaactggt gcccgcacga ccggacggta ctcgccaacg agcaagtcgt
     3661 gaacggacgc tgctggcgct gcgaaacgcc cgtcgtccgt cgcaatctct cgcagtggtt
     3721 cctcaagatt accgactacg ccgaccgtct actgaccgat ctggataaac tcgacggctg
     3781 gccggagcgc acgcgcacga tgcagcgcaa ctggatcggg cgcagcgaag gcgtgcagct
     3841 ggcgtttgcg gtcgacggac tcgacgccgc catcgaagtc tttacgacgc gcgtcgacac
     3901 gctgtacggc gcgacgttct tggcggtcgc accggaacac ccgctcgttt cgcgtctggg
     3961 ggaagtcgtt acgcgcgaag aagccgcggc cgtggcggcg ttcgccgagc gcgtatccgc
     4021 gcaatcggag ttggagcgca ccagcctatt ggaaaagcag gggctattca ccggagcgta
     4081 cgcgatcaat ccgctgtcgc acgagcgcat tccgatctgg gtaaccaact acgtgctggc
     4141 cgactacggc accggagcgg ttatgggcgt gcccgcgcac gacgagcgcg atttcgattt
     4201 cgcgcacacc cacgggctcc cggtcgcgtg cgtcgtcgtc ccggccggcg agttggaaga
     4261 cggcaccttg ccgcagctgc cgttcgtgga agacggacgt ctgatcgcca gcggcgactt
     4321 cagcacgatg tcgagcgcgc gcgcgcgcga aacgatcggc gcgcgactgc tcgccctcgg
     4381 ggttggaaaa ccggcggtga ccacgcggct gcgcgactgg ctcgtttcgc ggcagcgcta
     4441 ttggggaacg ccgattccga tcgtctactg cgagaa
//
LOCUS       DASUCJ010000251         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_827853, whole genome shotgun sequence.
ACCESSION   DASUCJ010000251 DASUCJ010000000
VERSION     DASUCJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827853"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFU90_03105"
     CDS             complement(<1..516)
                     /locus_tag="VFU90_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478789.1"
                     /translation="MVRSALSPPRRIGQGPWPLAFYVPAVTVVAASFLAALPIVSTVG
                     WYPDFGYLVFIGWRLLRADPWPAWWAAPLGFVNDLFTGYPIGFSIALWSATMLVLDLI
                     DRRTMWRDYWIEWVVAAVLIAIDEWLQWRIAETVAGGLPFTAMVPPLVISICAFPAFA
                     WVIWRIDAWRLG"
     gene            complement(516..>1050)
                     /gene="mreC"
                     /locus_tag="VFU90_03110"
     CDS             complement(516..>1050)
                     /gene="mreC"
                     /locus_tag="VFU90_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5478790.1"
                     /translation="KATLELREHERKTVAIGRVVGSSFNSPRRFAILSAGSSDGIAVG
                     MPVRSPDGLVGRIMDVGALASRVLLVSDRANIVPARLLRNGIPVIAQGRGDGTIDVRP
                     LEVGRNPFKPGDIIDSSGTGGLYPPSVPVARVVKLQGDDAIALPLADPATISFAIVEP
                     PFEPVAMAAQAAATDAP"
BASE COUNT          208 a          333 c          366 g          143 t
ORIGIN      
        1 gccgagcctc cacgcgtcaa tgcgccagat gacccaggcg aacgccggga aagcacagat
       61 ggagatgacg agcggcggca ccatcgctgt gaacggcaga ccgcccgcga cggtttcggc
      121 aattcgccat tgcagccatt cgtcgatcgc gatcagcacc gctgcaacca cccactcgat
      181 ccagtaatcg cgccacatgg tgcggcggtc gatcagatcg agcaccagca tggtcgcgct
      241 ccacaaagct attgaaaagc ctatgggata accggtgaac aggtcgttga cgaaaccgag
      301 cggagcggcc caccaggcgg gccagggatc ggcccggagc agccgccagc cgatgaagac
      361 gaggtagccg aagtcgggat accagccgac ggtcgagacg atcgggagcg ccgcgagaaa
      421 ggaggcggca acgaccgtca ccgcgggcac atagaacgcg agcggccacg ggccctgtcc
      481 gatccgccgc ggcggactga gcgcggagcg gaccatcagg gcgcgtcggt cgcggcggcc
      541 tgagcggcca tggcgacggg ttcgaacggc ggctcgacga ttgcgaagct gatcgttgcg
      601 ggatcggcga gcggaagcgc gatcgcatcg tcgccctgga gcttgaccac gcgcgccacc
      661 gggaccgatg gcgggtagag accgccggtg cccgaactgt cgatgatgtc gccgggtttg
      721 aagggatttc gtccaacctc aagcggccgg acatcgatcg tgccgtcgcc gcggccctgc
      781 gcgatcacgg gaatgccgtt tcgaagcagt cgcgcaggga cgatgttcgc tcgatcggag
      841 accagcaaca cgcgcgaggc gagcgcgccg acgtccatga ttcgaccgac gaggccgtcc
      901 ggcgagcgga cgggcattcc aacggcgatt ccgtcgcttg agccggcgga gaggattgcg
      961 aagcgccgcg ggctgttgaa cgacgagccg acgacgcgcc cgatcgctac ggttttgcgc
     1021 tcatgctctc gcagttcgag cgttgccttc
//
LOCUS       DASUCJ010000252         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1500473, whole genome shotgun sequence.
ACCESSION   DASUCJ010000252 DASUCJ010000000
VERSION     DASUCJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500473"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFU90_03115"
     CDS             <1..532
                     /locus_tag="VFU90_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478791.1"
                     /translation="AGNEAVMALSPDAGTVGGQAPLNVLFDLTTGKNTQFSGYNGGPF
                     HAGYVNGLAVDPNTGVAVTDTELNAQVEFYDLKKRTGITAVQLPCTGPTDQGNSGAGI
                     TADPKNKLFLVTDPFYCSGSQGSAIVVYDEAGNVVETLTGFKFAIAEPPVAINPSKRM
                     GWAFGPAFSQLQQFFY"
     gene            672..>1370
                     /locus_tag="VFU90_03120"
     CDS             672..>1370
                     /locus_tag="VFU90_03120"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEU5478792.1"
                     /translation="MIRTVLWAAALAAASLAWLLPSGARALEKIPFTEDGGLIKVQGS
                     VDGEPVPMLVDLGAGLNVLSGRLGSRLVNVDGKYVTLSLTGQRVDVPIGTVVSIALGE
                     FKVDNRIVGIWKGLDGTGVDGLISANAFSNVTATFDYRAHELAIEDAVTFADRKRFAV
                     RVPLFLQDELGIALGVFARFNFGNGQSGLCVIDTGMPGVAIDRRFAKKLGVNLEGQPR
                     THTQLGDGVTAAIPE"
BASE COUNT          243 a          433 c          458 g          236 t
ORIGIN      
        1 ggccggcaac gaggccgtga tggcgctctc acccgacgcc gggacggtcg gcggacaggc
       61 accgctcaac gtgctgttcg acctgacgac cgggaagaac acgcagtttt cgggctacaa
      121 cggcgggccg ttccacgccg gctacgtcaa cggcttggcg gtcgatccca ataccggcgt
      181 cgcggtcacc gataccgagc tgaacgccca agtcgagttc tacgatctca agaagcgcac
      241 aggaatcacg gcggtacagc tgccgtgcac cggcccgacc gatcagggca acagcggcgc
      301 cggaatcacc gccgacccca agaacaagct gtttctcgtt accgacccgt tctattgcag
      361 cgggagccag ggcagcgcga tcgtcgtgta cgacgaagcc ggcaacgtcg tcgagacgct
      421 gaccggattc aagttcgcga tcgccgagcc gccggtcgcg atcaaccctt ccaagcgcat
      481 gggctgggcg ttcggcccgg cgttcagcca gctgcagcag ttcttttatt aaggagccct
      541 tcgtccttcg actgcgcgcc tgacggcgct ccgctcagga tgacagcgct gtgccgctcc
      601 gctcaggatg acagttctta ggatctgccg aagcgcctcg actcgcataa gacggttgag
      661 gagcgcctat catgattcgc acggttctgt gggcggcggc tttggccgcg gcctcgctgg
      721 cgtggctgtt gccgagcggc gcgcgcgcgc tcgagaagat tccgttcacc gaagacggcg
      781 ggctgatcaa agttcaggga agcgtcgacg gcgagccggt gccgatgctg gtggacctcg
      841 gcgcgggtct caacgtgctt tcgggacggc tcggctcgcg gctggtcaac gtcgacggga
      901 agtacgtcac gctgagcctt accgggcagc gcgtcgacgt gccgatcggc accgtcgttt
      961 cgatcgcgct gggtgagttc aaggtcgata accggatcgt cggcatttgg aagggcctcg
     1021 atggaaccgg cgtcgacggg ctgatctcgg cgaacgcctt cagcaacgta acggcgacct
     1081 tcgactatcg cgcgcacgaa ctggcgatcg aggacgcggt gacgtttgcc gaccgcaagc
     1141 gattcgccgt ccgcgtgccg ctctttctgc aggacgagct cgggatcgcg ctcggcgtat
     1201 tcgcccggtt caacttcgga aacgggcaga gcgggttgtg cgtcatcgac accgggatgc
     1261 cgggcgttgc gatcgaccgg aggtttgcga aaaagctggg cgtcaacctc gaaggtcagc
     1321 cgcgcaccca cacgcagctc ggcgacggcg taaccgcggc gattccggag
//
LOCUS       DASUCJ010000253         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_766925, whole genome shotgun sequence.
ACCESSION   DASUCJ010000253 DASUCJ010000000
VERSION     DASUCJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766925"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1120)
                     /locus_tag="VFU90_03125"
     CDS             complement(131..1120)
                     /locus_tag="VFU90_03125"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU5478793.1"
                     /translation="MLAASAAAASLIAQSIVWDNHTCMPLRPHDERFLPQLERLARSG
                     VTVVGVNIGFGEQSIEDHIRVLAHFRRWIAQRADRYVLVRTVDDVLGAKASGRLGIFF
                     DIEGARAIDDQPSMIGLYYDLGVRWMLMAYNRNNRVGGGCLDDDPGLSDFGYAVIDEM
                     ERAGMLACCSHTGERTTMDVIAYSRNPVIFSHSNPRALWDHPRNIRDEVIQACAAKGG
                     VVGINGVGQFLGKNDTSSRTFARNVDYVARLAGVDHVALGLDYVFDEQELADYLASMP
                     ATFPAGSHAPCDFVRPEQIAEIVGELLAMEYSHDDLTKILGGNLLRVARTVWK"
     gene            1204..>2056
                     /locus_tag="VFU90_03130"
     CDS             1204..>2056
                     /locus_tag="VFU90_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5478794.1"
                     /translation="MELRRTLGLTDVVLFFVVAGSNLQWVATAAAAGPSSIGVWVLGG
                     LAMFLPLSICVVYLTSRHPDEGGLYVWSKLAFGPFAGFFTGWTYWASNLPYFPALLYF
                     AAGNALYAGTGGGALAGSPWYFIAVAVFGLTLATVVNVLGLDVGKWLNNLGATTRWIA
                     TLILIAIGVAAWWKFGPAMPIDAHALRPGFAFKDLIFWSVIAFAWTGPEAISFVGGEI
                     RNPRRTIPYGLAMAAPAIGIIYIAGTMAVLAILPAHQVNASSGVMQAVALGAARLGWS
                     VLTPVAAI"
BASE COUNT          317 a          660 c          695 g          384 t
ORIGIN      
        1 gccgcgagga tcgccgtcgc tgcccacagc cgccctcgcc gcgataacat gaccctggca
       61 ttctgcccgt cgcacgacgc ggcgtaacga gaagcggtag ggcgctcgcc cggcgggcga
      121 actcagccgc ttacttccac accgtcctgg cgacgcgcag aaggttgccg ccgagaatct
      181 tcgtgagatc gtcgtgcgag tattccatcg caagaagctc gccgacgatc tcggcgatct
      241 gctccggacg gacgaaatcg cacggtgcgt gcgagcccgc cggaaacgtc gccggcatcg
      301 aggcgagata atcggcgagc tcttgctcgt cgaatacgta atcgagcccg agcgcgacgt
      361 gatcgacgcc ggccaagcgc gccacgtaat cgacgttgcg cgcgaacgtg cggctgctcg
      421 tgtcgttctt gcccaagaat tgaccgacgc cgttgatgcc gacgacgccg cccttggcgg
      481 cgcacgcttg gatgacctcg tcgcggatgt tgcgcggatg gtcccacagc gcgcgcggat
      541 tcgaatgcga gaagatcacg gggttgcggg agtaggcgat gacgtccatc gtcgtgcgtt
      601 cgccggtatg cgaacagcac gcgagcattc cggcgcgctc catttcgtcg atgacggcgt
      661 aaccgaaatc cgagagcccc ggatcgtcgt cgaggcagcc tccgccgacg cggttgttgc
      721 ggttgtaagc catcagcatc cagcggacgc cgagatcgta gtacaagccg atcatgctgg
      781 gctgatcgtc gatcgcgcgc gcgccctcga tgtcgaagaa gattcccaac cgtccgctcg
      841 ctttggcgcc gagtacgtcg tcgaccgtcc tgacgagaac gtaccgatcg gcgcgctgcg
      901 cgatccaacg gcgaaagtgc gcgagcacgc ggatgtgatc ttcaatcgat tgctcgccga
      961 agccgatgtt gacgccgacg accgtgaccc cgctgcgcgc cagccgctcg agctgcggca
     1021 agaagcgctc gtcgtgggga cgcagcggca tgcaggtgtg attgtcccac acgatcgatt
     1081 gcgcgatcag cgacgcggct gcggcggatg ctgcgagcac gatggtccgg tttatgcggg
     1141 cgccgcacgg cacctgcggg cgttggtaaa ggggctcgcc gggcggtccc cgcatcgtgc
     1201 ggtatggagc tgcgccgcac gctcggcctt accgacgtcg tcctgttttt cgtcgtggcc
     1261 ggctcgaatc tgcagtgggt cgcgacggcg gccgcggcgg gtccgtcgtc gatcggcgtg
     1321 tgggtcctcg gcgggctggc gatgttcctt ccgctttcga tttgcgttgt ctatttgacg
     1381 tcgcggcatc cggacgaggg cggcctgtac gtctggagca aactcgcatt cggcccgttt
     1441 gccggatttt ttaccggctg gacgtactgg gcgtcaaacc tgccgtattt cccggcgttg
     1501 ttgtatttcg cagcgggaaa cgcgctctac gccggcaccg gcggcggcgc gctcgcgggt
     1561 tcgccgtggt acttcatcgc ggtcgccgtc ttcgggctca cgctcgcgac cgtcgtcaac
     1621 gtactcggcc tcgacgtcgg caagtggctc aacaacctcg gcgcgacgac gcggtggatc
     1681 gccaccttga tcctgatcgc gatcggcgtc gccgcatggt ggaagttcgg tccggcgatg
     1741 ccgatcgacg cgcacgcgct gcgccccgga tttgcgttca aggacctcat cttctggtcg
     1801 gtcatcgcgt tcgcgtggac cggaccggag gcgatatcgt tcgtcggcgg cgagatccgc
     1861 aatccgcggc ggacgattcc ctacgggctg gcgatggccg cacccgccat cggcatcatt
     1921 tatattgcgg gaacgatggc ggtcttggcg atcctgccgg cgcatcaggt caacgcgtcg
     1981 tccggcgtaa tgcaagcggt cgcgctcggc gcggcgcgcc tgggctggag cgtgctgacg
     2041 ccggtggccg cgatct
//
LOCUS       DASUCJ010000254         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_904404, whole genome shotgun sequence.
ACCESSION   DASUCJ010000254 DASUCJ010000000
VERSION     DASUCJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904404"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFU90_03135"
     CDS             <1..886
                     /locus_tag="VFU90_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478795.1"
                     /translation="LTLHGMHYTGYVFNDFARMLEIVRWAAARMAGERVLAFLPSWDG
                     RYYWDYPRYRAADRLGGDAGLRRLVNEGHALGVRFMPMFGANAANRHAENFGRIAGAA
                     TAKIDGDPLDLNWVDWDNDRHQEGWLSYMNLGVDAWRGWLSARILDAIERYDVDAYFL
                     DIAGGWVNNPRADMHEGTRRLVHDIARAHPHVLACGEMHYDALLSFLPLYQSFSQWEV
                     RDEVERYARFFQHLSHPAPGRGSSGVHESGFNAWNPHTLSLREGQIPTLAIVDDTFST
                     YRSEMEAVVAKAKEMAGA"
     gene            complement(895..1947)
                     /locus_tag="VFU90_03140"
     CDS             complement(895..1947)
                     /locus_tag="VFU90_03140"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU5478796.1"
                     /translation="MRLASMIYAVDAHACGEPGRVIVGGVLDVPGASMFEKMCHLREH
                     ADALRLRMLREPRGYPAANCNVILPPTHPEADAGFVIMEQVEYPGMSGTNTICVTTVL
                     LVTGMLPMREPVTELTLETPAGLVRVRADCVDGKVQRVTFRNVPAFAVHLDAPVEVPH
                     LGTVPVDVAYGGMFYVIADAGRFGLRLTPDEGRDIVRLGEMIKAAAREQLPVSHPEQP
                     GFNGITIAQLSGAPSSPGAHARNAVVVSTGTLDWNRPATWTGAIDRSPCGTGTCAKMA
                     TLHARGLLPLERDFQHEGVLGTVFTGRLVAETMVGAYRAVVPTLSGQAWITGFSSYVL
                     DPTDPFPEGFTVGDIW"
BASE COUNT          353 a          689 c          741 g          340 t
ORIGIN      
        1 gttgacgctg cacggcatgc actacacggg ctacgtgttc aacgacttcg cgcgcatgct
       61 cgagatcgtg cgttgggccg cggcgcgcat ggcgggcgaa cgcgtgttgg cgtttcttcc
      121 gtcgtgggac ggccggtatt actgggacta tccgcggtac cgcgccgccg atcggttagg
      181 cggcgacgcc ggcttgcggc gtctggtgaa cgaaggacac gcgctcggcg ttcgcttcat
      241 gccgatgttc ggcgcgaacg cggcgaaccg gcacgcggaa aacttcggcc ggatcgccgg
      301 agccgcgacg gcgaagatcg acggcgaccc actcgacttg aattgggtcg actgggacaa
      361 cgaccgccat caggagggat ggctgtcgta catgaatctc ggtgtcgatg catggcgcgg
      421 ctggttgtcc gcccggattc tggacgcgat cgagcgctac gatgtggacg cctactttct
      481 cgacatcgcc ggtggttggg tgaacaaccc gcgcgccgac atgcacgagg gcacgcgtcg
      541 cctggtgcac gacattgcgc gcgcgcatcc gcacgtcctc gcgtgcggcg agatgcacta
      601 cgacgccctg ctctcgttcc tgccgctcta ccagtcgttc tcgcaatggg aggttcgcga
      661 cgaggtcgag cggtatgcgc ggttcttcca gcacttgagt catcccgcgc cggggcgcgg
      721 cagcagcggc gtgcacgaat ccggattcaa cgcgtggaac ccgcacacgc tgtcgctccg
      781 cgagggtcag attccgaccc tcgccatcgt cgacgacacg ttctcgacgt accggagcga
      841 gatggaggcc gtggtcgcga aggcgaaaga gatggcgggt gcctaactca cgcgtcacca
      901 gatgtcgccg acggtgaagc cctccgggaa cgggtcggtc gggtcgagga cgtacgagct
      961 gaatccggta atccaggctt gtccgctgag ggtcggcacg acggcgcggt aggcgcccac
     1021 catcgtctcg gccacgaggc ggccggtgaa taccgtgcct aacactccct cgtgctggaa
     1081 atcgcgctcc agcggcagca gtccgcgggc gtgcagcgtc gccatcttcg cgcaggtgcc
     1141 cgtgccgcac ggtgagcggt cgatcgcgcc ggtccaggtg gccggccggt tccagtcgag
     1201 cgtgcccgtc gacaccacca ccgcattgcg cgcgtgcgcg cccggcgacg acggggcgcc
     1261 ggacagttgg gcgatggtga tgccgttgaa gcccggctgc tcgggatgcg acacgggcaa
     1321 ctgctcgcgg gccgccgcct tgatcatctc gcccaggcgc acgatgtcgc gtccctcgtc
     1381 cggcgttagg cggaggccga agcggccggc gtcggcgatc acgtagaaca tcccgccgta
     1441 cgcgacgtcc accggcaccg tgcccaggtg tggcacttcg accggcgcat cgaggtgcac
     1501 ggcgaacgcg ggcacgttgc ggaacgtgac gcgctgcacc ttgccgtcga cgcagtccgc
     1561 gcgcacgcgc acgagacccg cgggcgtctc gagggtcagc tcggtcaccg gctcgcgcat
     1621 gggaagcatg ccggttacga gcaggacggt ggtgacgcag atcgtgttgg tgcccgacat
     1681 gccgggatac tcgacctgct ccatgatcac gaagcccgca tcggcctcgg gatgcgtcgg
     1741 tgggagaatg acgttgcaat tggcggcggg atagccgcgc ggctcgcgca gcatgcgcag
     1801 ccgcagcgcg tcggcgtgtt cgcgcagatg gcacatcttc tcgaacatcg atgcgccggg
     1861 cacgtccagc acgccgccga cgatcactcg accgggctcg ccgcaggcgt gcgcgtcgac
     1921 ggcgtagatc atggatgcga gtcgcatcgc gctacagcca ggcgtggaag cggcggatgt
     1981 accaggtctt cacgacctga gtgagcaacc cgtacgacaa cagaatgccg atcagccaca
     2041 ggaagtagct gccgggcagc ggctcgaggc cgacgcgttc gcctaacgca atgaacggga
     2101 gcgcgatgcc gatcaccatg atc
//
LOCUS       DASUCJ010000255         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_94510, whole genome shotgun sequence.
ACCESSION   DASUCJ010000255 DASUCJ010000000
VERSION     DASUCJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94510"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFU90_03145"
     CDS             <1..477
                     /locus_tag="VFU90_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HEU5478797.1"
                     /translation="QVEHPVTEMITGLDIVQEQIRIAAGQKLRLRQRDIAYRGHAIEC
                     RINSEDPWTFVPSPGRITAWHAPGGPGIRVDTHVYQNYFVPPHYDSMIGKVVAYGDTR
                     EQAIARMRVALSEMVVGGIKTNIPLHQELLTDEKFLRGGTSIHFLEERLAQRAQAG"
     gene            482..1432
                     /gene="prmA"
                     /locus_tag="VFU90_03150"
     CDS             482..1432
                     /gene="prmA"
                     /locus_tag="VFU90_03150"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5478798.1"
                     /translation="MVRAPQATYRALRIDVTARDADAWSDALLAAGAMSVDAGDPDAG
                     TAHETPLYEEGVGDEPAWWPVCCLVALFGPGDDPVRALASAARALQRPVPASRVDAIP
                     DRDWVAATRAQYAPIEAAPGLWIVPSWCEPPVGDAVTVILDPGVAFGTGSHPTTRMCL
                     AWLARTVRAGDSVLDYGCGSGILAIAAAKLGATRVEGVDVDPQAIGVSGDNARANGIA
                     AHFHLADERRESHRTQCDLQSDVVVANILANPLRLLAPLLASRCRANGRIALAGILDA
                     QADEVIDAYARWFTMRREEFREGWVLLAGVRACVPGVNRP"
     gene            1436..2452
                     /locus_tag="VFU90_03155"
     CDS             1436..2452
                     /locus_tag="VFU90_03155"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEU5478799.1"
                     /translation="MSDLQFTRCPGCATVYRVTGAQLALREGQVRCGHCRAVFDATAH
                     RVDLAPTAPPADGSAHPEVDDELARGRATVTLREASALEAPEREAPERPASSEQAVQT
                     GQAADAEKAADAEQEPAAAEQEPAAAASPVPEASPPEVSTPDDGGPAPTTAEGASSDA
                     PLDIAELEAARAALRPRRHARSGKVFHLIALAVLVVGLVVQSAALFRDDVVARMPQAV
                     GALDALCRALTCRTGPRHDAGALSIEGSDLQADPAHRGLLVLTATLRNRAGRAVAWPD
                     LELTLTDSSDQAVVRRVLTPNDYLAVGTRSPGLAAHSERQVRLFLDASATRQAGYRLY
                     LFYP"
     gene            2732..>4083
                     /gene="glnA"
                     /locus_tag="VFU90_03160"
     CDS             2732..>4083
                     /gene="glnA"
                     /locus_tag="VFU90_03160"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5478800.1"
                     /translation="MTTAADVLTLIKDKEVKFVDLRFTDTRGKEQHVSVPAKAFDADK
                     FESGHAFDGSSIAGWKGIQASDMLLLPDPATANLDPFMDDTTLLLTCDVIEPSDGKGY
                     ERDPRSLAKRAEAYLKSTGLGDIAYFGPEPEFFIFDSVRFDTTQQSSYHFIESNEGNW
                     NSGKENAIKGGLNLGYKVKNKEGYFPVPPTDSLQDIRTEMVLEMERVGIRVEKQHHEV
                     ATAGQAEIDMRFDSMTKMGDKMMVFKYIIKNVAAQHGYVATFMPKPLFQDNGSGMHVR
                     QSLWKDGQNLFADAEGYAGLSEMAVHYIGGLLKHAPALLAICAPTTNSYRRLVPGYEA
                     PINLVYSKRNRSACIRIPMYSTNPKTKRVEFRPPDPSANPYLAFAAMLMAGIDGILTK
                     AEPGEPLDKDIYDLSPEEMKNVPSMPGSLEEALDCLEEDHAYMLKGDVFTEELIETYI
                     "
BASE COUNT          721 a         1381 c         1324 g          657 t
ORIGIN      
        1 caggtcgagc atccggtcac cgagatgatc accggcctcg acatcgtgca ggagcagatc
       61 cgcatcgcgg ccgggcagaa gctccggctt cgccagcgcg atatcgcgta ccgcgggcac
      121 gcgatcgagt gccggatcaa ctccgaggat ccgtggacct tcgtgccgtc gccgggacgc
      181 atcaccgcgt ggcacgcacc cggcggtcct ggcatccgcg tcgatacgca cgtctaccag
      241 aactacttcg tcccgccgca ctacgactcg atgatcggca aggtcgtcgc ctacggagac
      301 acgcgcgagc aggcgatcgc gcggatgcgg gtggcgctct ccgaaatggt cgtgggcggc
      361 atcaagacga acatcccgct ccaccaggag cttctcaccg acgagaaatt ccttcgcggc
      421 gggacgtcga tccattttct cgaggagcgg ctcgcgcagc gggcgcaggc tggctagtca
      481 gttggtccgg gcgccccagg cgacgtaccg cgcgctgcgc atcgacgtca cggcgcgcga
      541 cgccgacgca tggtcggacg cgctgctcgc agcgggcgcg atgtcggtcg atgcgggcga
      601 tcccgacgcg ggaacggcac acgagacgcc gctctacgag gagggtgtcg gcgacgaacc
      661 agcctggtgg ccggtttgct gtctcgtcgc gctgttcgga cccggtgacg atccggtccg
      721 cgcactcgcg tcggcggcgc gcgcgctgca gcgcccggtg ccggcaagcc gcgtcgacgc
      781 catccccgat cgtgactggg ttgccgctac tcgcgcacag tacgccccga tcgaagccgc
      841 tccgggactg tggatcgtgc cgagctggtg cgagcccccg gttggcgatg ccgtcaccgt
      901 catcctcgac cccggcgtcg cgttcggaac gggctcgcat ccgacgacgc ggatgtgcct
      961 cgcctggctc gcacgcacgg tgcgggccgg cgactcggta ctcgactacg gttgcgggtc
     1021 ggggatcctc gccatcgcgg cagcgaagct cggcgcgaca cgcgtcgaag gcgtcgacgt
     1081 cgatccgcag gcgatcggcg tgagcggcga caacgcgcgc gcgaacggca ttgcggcgca
     1141 ttttcatctt gccgacgagc ggcgcgaatc gcaccgaacc cagtgcgacc ttcagtccga
     1201 cgtcgtcgtt gcgaacattc tcgccaatcc gctgcgtctc ctcgcgccgc ttcttgccag
     1261 tcgttgccgc gccaatggtc gcatcgcgct cgcgggcatt ctcgacgctc aggcggatga
     1321 ggtgatcgac gcgtacgcgc gctggtttac gatgcgacgc gaagagttcc gcgaaggctg
     1381 ggtgcttctc gcgggcgttc gcgcttgcgt acctggcgtt aacagaccct gattcgtgag
     1441 cgacctgcaa tttactcgct gccccggatg tgcgaccgtc tatcgcgtga ccggcgcgca
     1501 gctcgcgctg cgcgaagggc aggtccgctg cggtcactgc cgcgcggtgt tcgacgcgac
     1561 ggcacatcgc gtcgacctcg cgccgaccgc accgcctgcc gacggatcgg cgcatcccga
     1621 agtcgacgac gagctcgcgc gcgggcgcgc gacggttacg ctgcgcgagg cgagcgcgct
     1681 cgaggcgccg gagcgagagg cgcctgagcg accggcaagc agcgagcaag cagtccaaac
     1741 ggggcaggcg gccgacgcgg agaaggcggc cgacgcggag caggagcctg ccgccgcgga
     1801 gcaggagcct gccgccgcgg cgtcgccagt tccagaggca tcgccgccgg aggtatcgac
     1861 gccggacgac ggggggccgg cgccgacgac cgcggaaggc gcctcgtccg atgctccgct
     1921 cgacatcgcg gagctcgagg ctgcccgcgc ggctcttcgg ccgcgtcgcc acgcccgcag
     1981 cggcaaggtg ttccatctga ttgcgctcgc cgtgctggtc gtaggactgg tggtgcagtc
     2041 ggcggcgcta tttcgcgacg acgtcgtggc gcggatgccg caggcggtcg gcgcgctcga
     2101 cgcgctgtgc cgggcactga cctgccggac cgggccgcga cacgatgccg gcgcgctctc
     2161 gatcgagggc tccgatctgc aagccgatcc ggcgcaccgt gggctcctcg tgctgacggc
     2221 gacgcttcga aatcgcgccg gccgggcagt cgcgtggccc gacctcgagc tcacgctgac
     2281 cgattcctcc gatcaggctg tcgtccgaag agtattgacg ccgaacgact atctcgccgt
     2341 cggcacgcgg tcgcccgggc tcgccgcaca cagcgaacgt caggtcagac tgttcctcga
     2401 tgcgagcgcg acgcgtcagg ccggctaccg cctctacctt ttctatccgt gacgctgcgc
     2461 gaggcatcgg ctggagccgt gcgcatgcgc gattgcacca aattggaaat cagcgtagcg
     2521 tgagcgcacc atagaggtgc gtcattcgtt gacaatactt ggagctcatt cgataaacgc
     2581 cgtcgaatca aagtgctggc tgcattaccg tgaatggcac agtcgtcgct aaattgcaga
     2641 cgaagcggag tgcggcgaaa ccgccaaggc cgtcgccata cctattgcca tccccatcgc
     2701 catccacaac ccatctccag gagagtcgtg catgaccact gccgccgatg tgctcacgct
     2761 gatcaaggac aaggaagtca agttcgtcga cctgcggttc accgatacgc gcggcaagga
     2821 gcagcacgtc tcggtgcccg cgaaggcatt cgacgccgac aagttcgagt cggggcatgc
     2881 gttcgacggc tcgtcgatcg ccggctggaa aggcatccag gcctccgaca tgctgttgct
     2941 gcccgatccc gcgacggcga acctcgaccc gttcatggac gacacgacgc tgctcctcac
     3001 ctgcgacgtg atcgagccgt cggacggcaa gggctacgag cgtgacccgc gctcgctcgc
     3061 caagcgcgcg gaggcctacc tgaaatcgac gggcctcggt gacatcgcct acttcggccc
     3121 cgagcccgag ttcttcattt tcgattccgt ccgcttcgat accacccagc agtcgagcta
     3181 tcacttcatc gaatcgaacg agggcaactg gaacagcggc aaggaaaacg ccatcaaggg
     3241 cggcctcaat cttggctaca aggttaagaa taaggagggt tacttcccgg tgcccccgac
     3301 cgatagcctc caggacatcc gcaccgagat ggtgctcgag atggagcgcg tcggcatccg
     3361 cgtcgagaag cagcatcacg aagtcgccac cgccggccag gccgagatcg acatgcggtt
     3421 cgattcgatg accaagatgg gcgacaagat gatggtcttc aagtacatca tcaagaacgt
     3481 cgctgcgcag cacggctacg tcgcgacctt catgcccaag ccgctcttcc aggacaacgg
     3541 gtccgggatg cacgtgcgcc agagcctctg gaaggacggg cagaacctct ttgccgacgc
     3601 ggagggctac gccggtctca gcgagatggc cgtccactac atcggcggcc tgctcaagca
     3661 cgcgccggcg ctgctggcga tctgcgcgcc gaccaccaac tcgtaccggc gtctcgtgcc
     3721 cggttatgag gccccgatca acctcgtcta ctcgaagcgc aaccgctcgg cgtgcatccg
     3781 catcccgatg tactccacca accccaagac caagcgcgtc gagttccggc cgcccgatcc
     3841 gtcagcgaat ccgtacctgg catttgcggc catgctgatg gcgggcatcg atggaatcct
     3901 caccaaggcc gaaccgggcg agccgctgga caaggatatc tacgaccttt cgcccgagga
     3961 aatgaagaac gtcccctcca tgcccggttc gctcgaagag gcattggatt gcctggaaga
     4021 agatcatgcc tacatgctga agggcgatgt cttcaccgaa gaactgatcg aaacctatat
     4081 caa
//
LOCUS       DASUCJ010000256         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_950163, whole genome shotgun sequence.
ACCESSION   DASUCJ010000256 DASUCJ010000000
VERSION     DASUCJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950163"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="VFU90_03165"
     CDS             <1..>1006
                     /locus_tag="VFU90_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU5478801.1"
                     /translation="PDKNGEIYLARVNWLPDASALTFQRETRDQQTLDLVSVDAGSLH
                     HRTVLTETSKTWVNLNDDLRFLRHDDAFVWASEQSGHKHLYVVGLDGKQRRALTEGDW
                     DVDKVLALDESNGTVYFTSGRDDPLQQQIYAVKLDGSEAASPRRISAGEGWHEAEFGE
                     NAQAYIDTYSDPATPPQVSLRKPDGGLVAWIEPNTLKEGHPYWAYRDSLIQPEFGSLK
                     SEDGQALLYRIYKPADFDAKKKYPVFMTYYGGPGRQYVNRAWGNHFEQYMAQHGYVVF
                     ALDNRGSPRRGRAFSDAIFRQLGKAEVADQLTGIAWLKQQRFVDGDHIGVFGWSYGGY
                     "
BASE COUNT          192 a          320 c          335 g          159 t
ORIGIN      
        1 cccctgacaa gaacggcgag atctatctcg cgcgcgtgaa ctggctgccc gatgcgagcg
       61 cgctgacgtt ccagcgcgag acgcgcgatc agcagacgct cgatctcgtt agcgtcgacg
      121 ccggcagcct gcatcaccgc acggtgctga ccgagacgag caagacgtgg gtcaatctca
      181 acgatgacct ccgtttcctc aggcatgacg atgcgttcgt gtgggcgtcg gagcagagcg
      241 ggcacaagca tctctatgtc gtcggcctcg acggcaagca gcggcgcgcg ctgaccgagg
      301 gcgactggga cgtcgacaag gtcctggcgc tcgacgaaag caacggcacg gtctacttca
      361 cgtcgggtcg cgacgatccg ctgcagcagc agatctatgc ggtcaagctc gacggcagcg
      421 aagcggcgtc gccgcgacgg atcagcgccg gcgaagggtg gcacgaggcc gagttcggcg
      481 agaacgcgca ggcgtacatc gatacgtatt ccgatcccgc gacgccgccg caggtttcgc
      541 tgcggaaacc ggatggcggg ctcgtcgcgt ggatcgagcc gaacacgctg aaggaaggcc
      601 atccgtactg ggcgtatcgc gacagcctga tccagccgga gttcggttcg ctgaaatccg
      661 aggacggcca ggccctgctc taccggatct acaagccagc cgacttcgac gcgaagaaga
      721 agtatccggt gttcatgacg tactacggcg gcccgggccg ccagtacgtc aaccgtgcgt
      781 ggggcaatca cttcgagcag tacatggcgc agcacggcta cgtcgtgttc gcgctcgaca
      841 atcgcggctc gccgcggcgc ggccgcgcgt tttccgacgc gatcttccgc cagctcggca
      901 aggccgaagt cgccgaccag ctgaccggca tcgcgtggct gaagcaacag cgcttcgtcg
      961 acggcgatca catcggcgtg ttcggctgga gttacggcgg ctacat
//
LOCUS       DASUCJ010000257         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_430626, whole genome shotgun sequence.
ACCESSION   DASUCJ010000257 DASUCJ010000000
VERSION     DASUCJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430626"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFU90_03170"
     CDS             complement(<1..807)
                     /locus_tag="VFU90_03170"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5478802.1"
                     /translation="MKLNTALSALLDVEVPLFNAPMTPQAGGELARAVSDAGAFGMLG
                     FDEDESEDAIAGQAALLREGRDRAFGIGLAAWVLERRPHLLDVAIAARPKLVSISFGD
                     PAPFVKTLHDAGILVAAQVQSRQWAQTAIDAGVDVVVAQGTEAGGHTGRVATLPLLQF
                     VLEMTDRPVVAAGGIATGRGFAAVLAAGAAGAWIGTPFLLAREARSNPQAQARLIAAD
                     ETQTVYTHVYDVLQEKPWPEEFAGRALRNPFVDRWHTHENELRETPAAAEE"
     gene            complement(804..>2234)
                     /locus_tag="VFU90_03175"
     CDS             complement(804..>2234)
                     /locus_tag="VFU90_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5478803.1"
                     /translation="KVSTAAYVNSLFRPEIIADLRLRDYGFEPIERDPASFSPFLDGR
                     YLMLGTGTSKDVDEISKFSKKDAENYPRYEAMLERVASIVEPTLVQVPPNLLHPTLQQ
                     LLQMGAMGKKLQRQGPAMGEAVEVLTGAARPILDRWFESEQLKGTLATDAIIGAFAAP
                     STPGTAYVLFHHVMGETNGKRGVWSYVRGGMGGLTQALARAGQDLGVEIRTEAEVAKI
                     LTENGRATGVALANGDEFYAKNVASGVDCHITFEKLLDPEALPPAFRDAIARISYASA
                     SCKINVALERLPSFSALPGHEPGPQHRGTVHLCPDQDFIERAYDDAKYGKMSQQPVVE
                     CTMPSSLDPTVAPPGKHLMSMFTQYAPYALADGPWTDAKRNEYADRCFDVVEQYAPGF
                     KASVIDRQILTPVDLESTFGLTGGSIFQGAMPLHQLFMFRPVPGFAGYATPVKGLYLC
                     GAAAHPGGGVMGASGWNAARVMLRRT"
BASE COUNT          339 a          742 c          768 g          385 t
ORIGIN      
        1 ttcctcggcc gcggcggggg tttcgcgcag ctcgttttcg tgcgtgtgcc agcggtcgac
       61 gaacggattg cgcagcgcgc gccccgcgaa ctcctcgggc cacggtttct cttgcagcac
      121 gtcgtacacg tgggtgtaaa cggtttgcgt ctcgtcggcg gcgatcagcc gggcctgcgc
      181 ttgtggattc gagcgcgctt cgcgcgccag caaaaacggc gtgccgatcc acgcgccggc
      241 ggcgcccgcg gcgagcaccg cggcgaagcc gcgtccggtc gcgatcccgc cggcggcgac
      301 gacggggcgg tcggtcattt cgagaacgaa ctgcaggagc ggcagcgtgg ccacccggcc
      361 ggtgtgcccg ccggcctccg tgccctgcgc gaccacgacg tcgacgcccg cgtcgatcgc
      421 ggtttgcgcc cactgccggc tctgtacttg cgcggcgacg aggatgccgg cgtcgtgcag
      481 cgttttcacg aacggcgccg gatcgccgaa cgatatgctg acgagtttgg gacgcgcggc
      541 gatcgcgacg tcgagcaggt gcggacgccg ctcgagcacc cacgccgcca acccgatgcc
      601 gaaggcgcgg tcgcgtcctt cgcgcagcag cgcggcttgc ccggcgatcg catcttcgct
      661 ttcgtcttcg tcgaagccga gcattccgaa ggcgcccgcg tcgctgacgg cgcgcgcgag
      721 ctcgccgccg gcttgcggcg tcatcggtgc gttgaacaac ggtacttcga cgtcgaggag
      781 cgccgaaagc gcggtgttga gcttcacgtt ctgcgcagca taacacgcgc cgcgttccag
      841 ccggatgcgc ccatcacgcc gccgcccggg tgagcggccg caccgcagag gtaaagcccc
      901 ttgaccggcg tggcgtagcc ggcgaagccg ggaaccggcc ggaacatgaa gagctggtgc
      961 agcggcatgg ctccctggaa gatgcttccg cccgtcaaac cgaacgtgct ctccaaatcg
     1021 accggcgtga ggatttggcg gtcgatgacc gatgccttga atcccggcgc gtactgctcc
     1081 accacgtcga aacaccggtc ggcgtactcg ttgcgtttgg catcggtcca cgggccgtcg
     1141 gcgagcgcgt acggagcgta ctgggtgaac atcgacataa ggtgcttccc gggcggcgcc
     1201 accgtcgggt cgagcgacga cggcatcgtg cactcgacga ccggctgttg cgacattttc
     1261 ccgtattttg cgtcgtcgta ggcgcgctcg atgaaatcct gatccgggca gagatgaacc
     1321 gtgccgcgat gctgcgggcc gggttcgtgc ccgggcagcg cgctaaagct cggcagacgc
     1381 tcgagcgcga cgttgatctt gcacgacgcg ctggcgtacg aaatgcgcgc gatcgcgtcg
     1441 cggaaggccg gcggcagagc ctccgggtcg aggagcttct cgaaggtgat gtggcaatcg
     1501 acgcccgacg cgacgttctt agcgtaaaat tcgtcgccgt tggcgagcgc cacgccggtc
     1561 gcgcgtccgt tttcggtaag aatcttggcg acttccgctt cggtgcgaat ctcgacgccg
     1621 agatcttgcc cggcgcgcgc cagcgcctgc gtgaggccgc ccattccgcc gcggacgtaa
     1681 ctccagacgc cgcgcttgcc gttggtctcg cccatgacgt ggtggaacag cacgtacgcc
     1741 gtgcccggcg tgctcggcgc cgcgaaagcg ccgatgatcg cgtcggtcgc cagcgtgccc
     1801 ttgagttgct ccgactcgaa ccagcggtcg aggatcggac gcgcggcgcc ggtgagaact
     1861 tcgacggcct cgcccatcgc ggggccttgg cgctgcagct tcttgcccat cgcccccatc
     1921 tgcaagagct gctgcagcgt cgggtgcagc aggttcggcg ggacttgcac gagcgtcggc
     1981 tcgacgatcg aggcgacgcg ctcgagcatc gcctcgtagc gcggatagtt ctcggcgtct
     2041 ttcttgctga acttcgagat ctcgtcgacg tctttgctgg tgccggttcc gagcatcaga
     2101 taccgcccgt cgagaaacgg cgagaacgac gcgggatccc gctcgatcgg ttcgaagccg
     2161 taatcgcgaa gccgcagatc ggcgatgatc tccgggcgga agaggctgtt gacgtacgcc
     2221 gcggtcgaca cttt
//
LOCUS       DASUCJ010000258         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1148820, whole genome shotgun sequence.
ACCESSION   DASUCJ010000258 DASUCJ010000000
VERSION     DASUCJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148820"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1316
                     /locus_tag="VFU90_03180"
     CDS             240..1316
                     /locus_tag="VFU90_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU5478804.1"
                     /translation="MRGDDTKQAALFSFVSPEQRVPQDHPLRPIKALTEQILKSLSRT
                     FSRMYSKTGRPSIPPERLLKAQVLMALYSVRSDRLFCEQLDYNLLFRWFLDMTMDEPA
                     FDHSSFTKNRERLLKHEVAATFFAAVVGEARERALMSNEHFSVDGTLIEAWASLKSFR
                     PRDEKPEDREPPDDPGNPTVNFHGEQRSNETHGSTTDPEARLARKGHGKEAKLCFSAH
                     ALMENRNGLAVDVVAAAATGTAEREVGLALISAHVPRGATVGGDKGYNQRAFVDGCRA
                     LGVTPHVAENVKYSAIDRRTTRHAGYAISMRIRKRIEEIFGWIKTTGGFRRTRVKGIA
                     LTQHALYVVGAALNLLRITRLAAA"
     gene            complement(1439..>1820)
                     /locus_tag="VFU90_03185"
     CDS             complement(1439..>1820)
                     /locus_tag="VFU90_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5478805.1"
                     /translation="YLDRTATPATPLVLCGDFNVTRDDKDVWSPEAWAGKIHCSPDER
                     AALDRVLEFGLVDLFRQHQPDGGVYSWWEYLGVSFFKNRGLRIDYVFATPPVAARCTA
                     CTIDRDARKGQDASDHAPVIATIA"
BASE COUNT          336 a          581 c          623 g          280 t
ORIGIN      
        1 aagatcaagg cggcgcaggc caccgtggtt accgatccga tggcgggcgc gatggtcgtc
       61 gcgcgcgtcg tgacgaagac gaagtgcggc gccgaagaga tcatcgatca ctgcgcgcgc
      121 aacctcgccc cctacaaggt cccgcggcgc atcgagttct gcgaagcgat ctagcagggc
      181 gttgaaaaag ccgtgtccgg gatcgacgtg atccgtcgat ccggatagcg tcggcgacga
      241 tgcgcggcga cgacaccaag caagcggccc tgttctcgtt cgtgagcccg gagcaacggg
      301 tgccgcagga ccacccgcta cgcccgatca aagcgctgac ggagcagatc ttgaaatcgc
      361 tgtcgcggac gttcagccgc atgtacagca agacggggcg accgtcgatt ccgccggagc
      421 ggctgctgaa ggcgcaggtc ctgatggcgt tgtactcggt gcgcagcgac cggctattct
      481 gcgagcagct cgactacaac ttgttgttcc gctggttcct cgacatgacg atggacgagc
      541 cggcgttcga ccacagctcg ttcacgaaga atcgcgagcg cctgttgaag cacgaggtcg
      601 ctgcaacgtt cttcgccgcg gtcgtcgggg aggcgcgcga gcgcgcgctg atgagcaacg
      661 agcacttcag cgtcgacggc acgctgatcg aagcatgggc atcgctcaag agcttccggc
      721 cgcgcgacga gaagcccgag gaccgcgagc cgccggacga tccgggcaac ccaaccgtca
      781 actttcacgg cgagcagcgc agcaacgaga cgcatggctc gacgacggat ccggaagccc
      841 ggctcgcccg caagggtcat ggcaaggagg ccaagctgtg cttctcggct cacgcgctga
      901 tggagaatcg caacgggctc gcggttgatg tggtcgccgc cgccgcgacg ggcaccgccg
      961 agcgcgaggt cggcttggcg ttgatttcgg cacatgtgcc gcgcggcgcg accgtcggcg
     1021 gcgacaaagg ctacaaccag cgtgcgtttg tcgacggctg tcgcgcgctc ggcgtgacgc
     1081 cccacgtcgc cgagaacgtg aagtactcgg cgatcgatcg gcgcacgacg cgccatgcgg
     1141 gctacgcgat cagcatgcgc attcgcaaac gcatcgagga gatcttcggc tggatcaaga
     1201 cgacgggcgg cttccggcgc acgcgggtca aaggcatcgc gctcacgcag cacgcactct
     1261 atgtcgtcgg agcagcgctc aatttgctgc gaattacgcg gttggcagcc gcgtagcccc
     1321 acgtgggctt gggacaacgg cagcggcgcg ggcagcagcg ggcgtgccgc agcgggaagt
     1381 actcgctact tgactccgtt tttccacgcc ctgctagata gccagcgcgc agcgagcgtt
     1441 atgcgatcgt cgcgatcacg ggcgcgtggt ccgacgcgtc ctgacctttg cgcgcgtcgc
     1501 gatcgatcgt acaggccgtg cagcgcgcgg cgaccggcgg ggtcgcgaac acgtagtcga
     1561 tgcgcaggcc gcggttcttg aagaagctga cgccgagata ctcccaccac gagtacacac
     1621 cgccgtcggg ctggtgctgg cgaaacagat cgacgagccc gaactcgagc acgcgatcca
     1681 gcgcggcgcg ctcgtcgggg ctgcagtgga tcttgccggc ccacgcctcg ggcgaccaca
     1741 cgtccttgtc atcgcgcgtg acgttgaagt caccgcacaa gacgagcggc gtcgcgggcg
     1801 tggccgtgcg atcgagatag
//
LOCUS       DASUCJ010000259         1424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1423967, whole genome shotgun sequence.
ACCESSION   DASUCJ010000259 DASUCJ010000000
VERSION     DASUCJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423967"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU90_03190"
     CDS             <1..297
                     /locus_tag="VFU90_03190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5478806.1"
                     /translation="SFVAVSGSRVIGTVLCGHDGRRGYLYHLAVEAAHRGHGVGRALA
                     TRALDALRRDGIEKCHLMVVAGNAEARAFWSALGWQLRSDVVLMSYTSPESPNA"
     gene            416..1096
                     /locus_tag="VFU90_03195"
     CDS             416..1096
                     /locus_tag="VFU90_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU5478807.1"
                     /translation="MLVIAAFAARTATAQSAPNTLSARERADGWTLLFDGRTTSGWRG
                     YKQQTMPAGWSVQDGTLTKSTETEDIVTTGEYGDFELDVDWKIGTGGNSGIFYRGTEE
                     YDHVYWSAPEFQLLDDANAPDGKSRLTAAGSDYGLYPSPAGIVKPAGEWNTTRIIVKG
                     AHVEHWLNGKKLLSYELWSPSWSAKVKKSKFAPYGHYGMAKKGLIAIQGDHPGVLALR
                     NFKLKELH"
BASE COUNT          237 a          434 c          506 g          247 t
ORIGIN      
        1 agcttcgtcg ccgtgtctgg atcgcgcgtg attggcaccg tgctctgcgg acacgatggc
       61 cgccgcggct atttgtatca tctcgctgtc gaggctgcgc atcgcggaca cggcgtcggt
      121 cgcgcgctcg ccacacgtgc gctcgatgcg ctgcggcgcg atggcatcga gaagtgtcat
      181 ctcatggtcg tcgccgggaa tgccgaggcg cgcgcgttct ggtcggcact gggttggcag
      241 ttgcgatccg atgtcgtgct catgtcgtac acgtcgccgg agtcgcccaa cgcgtaggca
      301 ttgcggcggt cgcgtcggcc gggatatggt acggccgctg atgtttccgc gactccaccc
      361 acacgcccaa ccgaggttgt ccatggtgtg ccgccgtatc gcacggagcc tgaccgtgct
      421 cgtcatcgcc gcgttcgcgg cccggaccgc cacggcgcag tccgcgccta acacactcag
      481 cgcgcgcgag cgggccgacg gctggacgct cctgttcgat ggccggacga cgtcggggtg
      541 gcgcggctac aaacaacaga cgatgccggc gggttggtcg gtgcaggacg gcacgctgac
      601 aaagagcacc gagacggagg acattgtcac caccggcgag tacggcgatt tcgagctcga
      661 cgtcgactgg aagatcggca ccggaggcaa tagcggcatt ttttatcggg ggacggaaga
      721 gtacgatcac gtgtactgga gcgcgccgga atttcagctg ctggatgatg ccaacgcgcc
      781 cgacggtaag agccgtctaa cggcggcggg ttccgactac gggctctatc cctcgccggc
      841 gggtatcgtg aagccggctg gagaatggaa cacgacgcgg atcatcgtaa agggcgcgca
      901 cgtcgagcac tggctcaacg gcaagaagct gctctcctac gagctgtgga gcccgtcgtg
      961 gtcggcgaaa gtgaagaaga gcaagttcgc gccttacgga cactacggga tggcgaagaa
     1021 ggggctgatc gcgatccagg gtgatcaccc gggcgtgttg gcgctgcgca acttcaagtt
     1081 gaaagagttg cactgacggt cggcatcgtc cgtcgaggaa tcgtgatgcg tctcgcgtcc
     1141 gtcactgcgg ggcgcgcatg tgcgcgcgcc ggatcgtctt gtgcatcggg ctaacgggcg
     1201 cggcagatgt ttgctgacgt gatcgccgcg cgagaccccg acccggatcg cgcgatctgt
     1261 cggacgcgga ggactcgacc gctcatcacg ccgggcgtgg caggccgcgg caccgaggtc
     1321 cgcgtgctcg gcgccgtgct catcgcgcga cgctcgggcg ttcgcgccgc gagcgctgcg
     1381 ttgggcaggc gatggaccgg tgcccaacga ggccgatgcg attt
//
LOCUS       DASUCJ010000260         1216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1271249, whole genome shotgun sequence.
ACCESSION   DASUCJ010000260 DASUCJ010000000
VERSION     DASUCJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271249"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..>1216
                     /locus_tag="VFU90_03200"
     CDS             20..>1216
                     /locus_tag="VFU90_03200"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEU5478808.1"
                     /translation="MTSGDNIWTDQSSRAELQVGSTAMQLGPQTALGVTELDDTHFQL
                     RVDQGEVEIHVRQLYDNDVLELDTPNETVSLSQPGDYRVDVSADGQTSIVTVRGGLAS
                     VATTAQSFAVQAGQSASITGTDNATYDLAAAPAYDDLDTWSQARDRSEDDAVSRRYVS
                     TEMTGAEDLDAYGTWHSTPLYGEVWVPARVRAGWAPYRYGRWAFVQPWGWTWVDDAPW
                     GFAPFHYGRWAYYGGQWVWVPGAVVARPVYAPALVAFVGGSHFAASISFGGEGGVAWF
                     PLAPGEAYVPSYRVSDRYVRDVNVTNVHVTNINVRNINRTTVINNINNQYNYYRDGRP
                     TPSVAYANRGAPHAFSAVPGQSFASAQNVRRNLVHADPRQFATAPVLPRGVPIQPTRA
                     SFAPPRP"
BASE COUNT          197 a          418 c          409 g          192 t
ORIGIN      
        1 ggcgtctctc aactatccca tgacatcggg cgacaacatc tggaccgacc agagctcgcg
       61 cgccgagctg caagtcggct cgaccgccat gcagctcggt ccgcaaacgg cgctcggcgt
      121 cacggaactg gacgacaccc acttccagct gcgcgtggac cagggtgaag tcgagattca
      181 cgtccgccag ctgtacgaca acgacgtgct ggagctggac acgcctaacg aaacggtctc
      241 gctctcgcag ccgggcgact atcgggtcga tgtctcggcc gatggccaga cgagcatcgt
      301 gacggtgcgc ggcggcctgg cgagcgtcgc cacgactgcg cagtcgttcg ccgtgcaggc
      361 cggacagagt gcatcgatca ccggcaccga caacgccacg tatgatctcg cggccgcgcc
      421 ggcgtacgat gatctcgaca cgtggagcca ggcgcgcgat cgcagtgagg acgatgcggt
      481 ctcgcgtcgg tacgtatcca ccgaaatgac cggcgccgaa gatctcgacg cgtacggcac
      541 gtggcactcg acgccgctct atggcgaagt gtgggtgccg gccagggttc gcgcgggctg
      601 ggcgccgtat cgctacggcc gctgggcgtt cgtgcagccg tggggctgga cgtgggtcga
      661 tgacgccccg tggggcttcg cgcccttcca ctatggtcgt tgggcgtact acggcggcca
      721 gtgggtctgg gtgcccggtg cagtcgtcgc gcgcccggtc tacgcgcctg cgttagtcgc
      781 gttcgtcggc ggctcgcact ttgcggcctc gatcagcttc ggcggcgaag gcggcgtggc
      841 gtggttcccg ctcgcgccgg gtgaggcgta cgtgccgtcg tatcgggtca gcgatcgcta
      901 cgtgcgcgac gtgaacgtga ccaacgtgca cgtcaccaac atcaacgtgc gcaacatcaa
      961 tcgcacgacg gtgatcaaca acatcaacaa ccagtacaac tattaccgcg acggccggcc
     1021 gacgccgagc gtcgcctacg ccaaccgcgg cgcgccccac gctttcagcg ccgtgccggg
     1081 ccagagcttc gccagcgcgc agaacgtgcg gcgcaacctg gtgcatgcgg acccgcgcca
     1141 gttcgctacc gcgccggtgc tgccgcgcgg tgtgccgata cagccgacgc gcgcgagctt
     1201 cgctccgccc cgcccc
//
LOCUS       DASUCJ010000261         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1363004, whole genome shotgun sequence.
ACCESSION   DASUCJ010000261 DASUCJ010000000
VERSION     DASUCJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363004"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..916
                     /locus_tag="VFU90_03205"
     CDS             152..916
                     /locus_tag="VFU90_03205"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5478809.1"
                     /translation="MSRVLAIVSQKGGVGKTTTAINLAAALARRGIKTLLVDVDPQGS
                     VRYGLGMLAPHYRTGVSDYLNESAELQDIIRPTTLPWLRVALAGSIADGGTHEDYHLK
                     FAASNRFTELLNRARERGYLVVVDTPPGLGPIVQRVLANSDHVLVPLQCEPAAVQTTA
                     QILRGIRGVVMENQQLTFEGILLTMFEPGNAVSERVAAHVRAQLPQLVFDIAVPRTMA
                     SVDAVAAGQPVVLRAPNDPASRAYTALADELAPRLQ"
     gene            922..1743
                     /locus_tag="VFU90_03210"
     CDS             922..1743
                     /locus_tag="VFU90_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478810.1"
                     /translation="MRRSLLALGGASAVWLAVACLDVSSPIAGITSISPILAPTPSVV
                     EHDTLRDTAGVVGLLRVYAFGPSGDTVRDAVVHFYVVDTTNKLHVDSLTGIVSGDTPS
                     PTGAVFARVWPANGKGFIDTPLDTIPVVAVPNSAVSDTNFTFAFSAGTSSDTNATGLI
                     SPPFGVVVKGSADTTIQKYVVGFELVRTPASNGSNAGPTAVLTSALSTTDTAYAITDA
                     LGRATVRLRLRKTALSGQVLGGVTDTAVVRFHVRYRGNPLPVTPADSIIITVHVT"
     gene            1823..>2084
                     /locus_tag="VFU90_03215"
     CDS             1823..>2084
                     /locus_tag="VFU90_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5478811.1"
                     /translation="MITSGGPRNSAPDTLNRLFFDAAQRFDKPDALQVKIGGRYEPIS
                     HRTLVERVRRTALGLEALGVHAGDRVAILSENRPEWAIADYAC"
BASE COUNT          346 a          702 c          678 g          358 t
ORIGIN      
        1 cgcgcgggtg ccgagaagca cctcgaggcg ttcctcgcga aaccgccgtc gagcccggag
       61 gccgaacggt gggtgcacca cgcccaggcg accctcgcgg ggctccggtc tggagcgaac
      121 accatctcag ccgacgagcc agagccctaa cgtgtcgcgt gtgctcgcga tcgtcagcca
      181 gaagggtggg gtgggcaaga cgacgacagc aatcaatctc gcagccgcgc tggcccggcg
      241 cggcatcaag acgctgctcg tggacgtcga tccccaggga tcggtacgtt atgggctcgg
      301 catgctcgcg ccgcactatc gcaccggtgt ctccgactat ctgaacgaga gtgcggagct
      361 tcaggacatc atccggccca ccacgcttcc gtggctgcgg gtcgcgttgg ctggctcgat
      421 cgccgacggc ggcacgcacg aggactacca cctcaagttc gccgcctcga atcgcttcac
      481 cgagctgctg aaccgggcgc gggagcgggg gtatctcgtg gtcgtcgata cgccgccggg
      541 cctcggcccg atcgtacagc gcgtgctcgc gaacagcgac catgtcctcg tgccgcttca
      601 gtgcgagccc gcggccgtgc agaccacggc acagatcctt cgcgggattc gcggagtcgt
      661 gatggagaat caacagctca ccttcgaggg aatcctgctc acgatgttcg agcctggaaa
      721 cgcggtgtcg gagcgagtgg cggcgcacgt gcgcgcgcaa ctgccgcagc tcgtgttcga
      781 tatcgccgtg cctcgaacga tggcgtccgt ggacgcggtc gccgctggac agccggtggt
      841 gctgcgcgca cctaacgatc cggcgtcgcg cgcgtacacc gcgctcgcgg acgagctggc
      901 gccgcgcctg cagtgacatc ggtgcgccgt tctctgctcg cgctcggcgg cgcgtctgcg
      961 gtctggctgg cggtggcctg tctcgacgtg tcgtcgccaa ttgccgggat cacgtcgatc
     1021 agcccgattc tggcgccgac gccgtcggta gtcgaacacg acacgctgcg cgacaccgcg
     1081 ggagtcgttg gtctgctccg cgtctacgca tttggcccta gtggggacac ggttcgggac
     1141 gcagtcgtgc acttctacgt ggtcgacacg acgaacaagc tgcacgtcga tagcctcacc
     1201 ggaatagtca gtggtgatac gccgtcaccg accggagcgg tgttcgccag ggtttggccg
     1261 gcgaacggca aaggcttcat cgacacgccg ctcgatacga tccccgtcgt cgccgttccg
     1321 aactccgcgg tgagcgacac gaactttacc ttcgcgtttt cggccggcac gtcctcggac
     1381 acgaatgcga cggggctcat ctcgccgccg ttcggggtcg tcgtgaaggg gagtgccgac
     1441 accacgatcc agaaatacgt cgtcgggttc gagctggtac gcacgcccgc gtccaacggg
     1501 agcaacgccg gcccgaccgc cgtcctcacc agcgcgctgt ccacgacgga taccgcgtac
     1561 gcgatcaccg acgcgttggg ccgcgcgaca gtgagattac gcctgcgcaa gacagcactt
     1621 tccggacagg tattgggtgg cgtcaccgac accgcggtcg tccgattcca cgtacgctat
     1681 cgcggcaatc ctttgccagt cacgcccgcc gacagcatca tcatcacggt tcacgtcacc
     1741 tgacatcgta accggctgat tacccgttcg cgcggttcgg gggtatactc tcggcgtact
     1801 ccacgcttca gggaggattc gcatgatcac cagcggcgga ccgaggaatt cggctcccga
     1861 tacgctgaac cggctgttct tcgacgccgc tcagcgattc gacaagcctg acgcactcca
     1921 ggtgaagatc ggcggacgct acgagccgat ctcgcatcgc acgctcgtgg agcgcgtgcg
     1981 ccgcaccgcg ctcgggctcg aggcgttagg cgttcacgcc ggtgatcgcg tcgcgatcct
     2041 gtcggagaac cgtccggaat gggcgatcgc cgattacgcc tgcc
//
LOCUS       DASUCJ010000262         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1225489, whole genome shotgun sequence.
ACCESSION   DASUCJ010000262 DASUCJ010000000
VERSION     DASUCJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225489"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>2234
                     /locus_tag="VFU90_03220"
     CDS             118..>2234
                     /locus_tag="VFU90_03220"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5478812.1"
                     /translation="MARFDLETLTESPSLELPGNCEARDAALDGLNGNVFVLCDQTIT
                     MFDAAGAEASKLSLAEASKMSLAGANAAFDRIVHDPQSRDLIVSGTGAALRVGTLGGL
                     ELRGPVDPTLATVRPAPFRIDPAVALLRPPDGGATWDERQELVLSVGAQCPSAACVIP
                     QSYFEHLELEIAINGVREASSKVDPVTRRAALRAGTVLRPGVNRIEAEVRDRFGHVGR
                     MQAASLTLLTSAAESGGTDRNVVAKAANKLPTVTLTAPIAGSVFASGSDVTISASASD
                     SDGTIVRVEFYRNGSTLIGTASTSPYTIVWSKAVTGSHSLTARAYDDRKGSTTSPAVA
                     IKVVDNQPPTVAITDPADGAFYAAGGPITLAADARDGDGSVTRLEFLDGTTTIAVLTA
                     APWTWTWSGAAPGPHTLTARATDDRGAVTTSGAANVIIGTPPRVVVAAPAPCAVVDAP
                     IDLRVIVDVYSASSAIASVEVFDGSTSVGAVLQPPWLFVIANASVGTHAITARATDVH
                     GLSTTSRPAIVEVRAANQPPTATVTSPSEGATFASGATVTLRADASDPDGNVASVLFH
                     TESGFAIGTVVQPPYQVDWKNLASGGYTVYARVVDDRNAVTTSAPVHFTVAANLPPTV
                     SLTAPADGATFGAPATISLGASASDPDGAIARVEFYSGTTLLGTDTSEPFTYDWSGVG
                     AGTYSLSARAYDNSGAMATSSAVS"
BASE COUNT          374 a          789 c          720 g          351 t
ORIGIN      
        1 cgcccaacgc catcggattc ctcgcgagaa cgaggcgaca ccggcgacga cccgcgttgg
       61 cagcagatcc tcgtcgactc gctacgcgat caggcgtgga tcatcggacc gcgacggttg
      121 gcgcgtttcg acctcgagac actcaccgag agcccttcgc tggagctccc cggcaattgc
      181 gaagcgcgcg atgccgcact cgacggtttg aacgggaacg tcttcgtgct gtgcgaccag
      241 acaatcacga tgttcgacgc cgcgggcgcc gaagcgtcta agttgtcgct cgcggaagca
      301 tcgaagatgt cgctggccgg cgcgaatgcg gcgttcgacc gcatcgtcca cgatccgcaa
      361 tcgagagacc tgatcgtgtc gggcaccggc gcggcactgc gtgtcggcac gctgggcggt
      421 ctcgagttgc ggggccctgt cgatcccacc ctcgcaacgg tgcgcccggc gccgttccgg
      481 atcgatcccg ctgtcgcatt gctgcgccca cccgacggag gcgccacgtg ggacgaaaga
      541 caggagctcg tgctctcggt cggcgcgcaa tgtccgtcgg cagcttgcgt gattccgcaa
      601 agctacttcg agcatctcga actcgagatc gcgatcaacg gcgtccgcga agcctcgtcc
      661 aaagtcgatc ctgtcacgcg acgcgccgcg ttgcgtgccg gcaccgtcct gcgcccgggc
      721 gtgaaccgga tcgaagcgga ggtgcgcgac cgtttcggcc atgtcggtcg catgcaggcc
      781 gcgtcgctga cgttgctcac ttcagcggcg gaatccggcg gcacggaccg caacgtcgtg
      841 gcgaaggccg cgaacaagct gccaacagta acgctgacgg cgccaatcgc cggcagcgtg
      901 ttcgcgtccg ggagcgacgt cacgatctcc gcgagtgcga gcgatagcga cggcaccatc
      961 gtacgggtgg agttctatcg gaatggcagc acgttgatcg gtaccgccag cacgtcccca
     1021 tacacgatcg tgtggtcgaa ggctgttacc ggaagccact cgctgaccgc gcgcgcatac
     1081 gacgatcgca agggcagcac gacgtccccc gccgtcgcga tcaaagtcgt cgacaaccag
     1141 ccgccgacgg tggccatcac cgatcctgcc gacggcgcct tctacgccgc cgggggcccg
     1201 atcacgctcg ccgccgacgc gcgcgatggc gacggctcgg tcacgcgcct cgaattcctc
     1261 gacggcacca cgacgatcgc cgttctaacc gcagccccgt ggacatggac ctggtccggc
     1321 gcagcgccgg gtccgcatac actgactgcc cgcgcgacgg acgatcgcgg ggcggtgacg
     1381 acgtcaggtg ccgcgaacgt gatcatcggc acgcctccac gcgtcgtcgt cgccgcaccg
     1441 gcgccctgcg cggtggtcga cgcgccgatc gatctgcgag tgattgtcga cgtctacagt
     1501 gcaagcagcg cgatcgcaag cgtcgaggtc ttcgacggat cgacgtccgt gggtgcggtg
     1561 ttgcagccac cgtggctgtt cgtgatcgcc aatgccagcg tcggaacgca cgcgatcacc
     1621 gcacgcgcga ccgacgttca tggcctgtcg acaacgtcgc ggccggcgat cgtcgaagtc
     1681 cgtgcggcca atcagccacc caccgcgacc gtcacgtcgc cctccgaagg cgcgaccttt
     1741 gcttccggcg caacggtgac cctgcgcgcc gacgccagcg accccgacgg caacgtcgct
     1801 tccgtgctgt tccataccga gagcggcttc gcgatcggaa ccgtcgttca gccgccgtac
     1861 caggtcgact ggaagaacct cgcgtccggc ggctacacgg tctacgcgcg cgtcgtcgac
     1921 gatcgcaatg cggtgacgac ttcggcgccg gtccatttca ccgtcgccgc caatcttccg
     1981 cccaccgttt cgctgacagc gccggccgac ggtgccacat tcggcgcgcc cgcgaccatc
     2041 agccttggcg ccagtgcgag cgatccggac ggcgcgatcg cgcgagtcga gttctactcg
     2101 gggacgacgc ttctcggtac cgacacgagc gagccattca cctacgactg gagcggcgtc
     2161 ggcgccggaa cgtactcgct cagtgcgcgc gcctacgaca actccggcgc gatggcgaca
     2221 agctcggcgg tctc
//
LOCUS       DASUCJ010000263         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_323351, whole genome shotgun sequence.
ACCESSION   DASUCJ010000263 DASUCJ010000000
VERSION     DASUCJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323351"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFU90_03225"
     CDS             complement(<1..830)
                     /locus_tag="VFU90_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEU5478813.1"
                     /translation="MLLALIQAAALAVPLLHADPPIDGTLSSQAWKSAQSVTLDWDLQ
                     GHKHAPQQTTAYVATDGKYLYVAFDAKQDGTITATQHTDDAGQGTDDDVEIDLWPGGT
                     NGFLYQFIANPIGTHYQYSSENSGYRPEWWSAGTVHDGGYVVTMKIPLAAIHGVNSKR
                     WLVQFGRKVESTGDIDVWSYDSNETDTTTSTYAGILTGLQVTAIRPQPRVELYGLGLA
                     GGKSIGGSTSRAGVDFSVPFTATSSFYATLHPDYSNVELDQQSISPTAFRRYLSEVRP
                     "
     gene            complement(833..>1191)
                     /locus_tag="VFU90_03230"
     CDS             complement(833..>1191)
                     /locus_tag="VFU90_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU5478814.1"
                     /translation="LAGVEVVGSDFNDSNLSGASLQNARLVADDFNGANLRNVNFQGA
                     ALCAHDDSVDDDGAVRYGRLHCSDFSGADLHGANFRGAQICDYAGRGRAGRCTALDAA
                     TLRNLGHANLDGAEGP"
BASE COUNT          207 a          378 c          380 g          226 t
ORIGIN      
        1 aagggccgca cctcgctcaa atagcggcgg aatgcggtcg gcgaaatcga ctgctgatcg
       61 agttcgacgt tcgaatagtc cggatgaagc gtcgcgtaga acgacgacgt cgcggtaaac
      121 ggtaccgaga aatccacgcc tgcgcgagag gtcgatccgc cgatggattt gccgcccgcc
      181 agacccaggc cgtagagttc gacgcgcggc tgcgggcgta tcgccgtgac ttgcagtccc
      241 gtgagtatgc ccgcgtacgt cgacgtcgtc gtgtccgttt cgttcgaatc gtacgaccac
      301 acgtcgatat cacccgtgct ctctactttg cggccgaact ggacgagcca cctcttcgaa
      361 ttcacgccgt gaatcgccgc gagcgggatc ttcatcgtga cgacgtagcc gccgtcgtga
      421 accgttcccg ccgaccacca ctcgggccgg tagcccgagt tctccgacga gtattgatag
      481 tgcgtgccga tgggattggc aatgaattgg tacagaaacc cgttcgtgcc gcccggccac
      541 aagtcgatct cgacgtcgtc gtccgtgcct tgcccggcgt cgtcagtgtg ctgcgttgcg
      601 gtaatcgtgc cgtcttgctt ggcatcaaat gcaacgtaca ggtacttgcc gtcggtcgct
      661 acgtacgcgg tggtctgctg gggcgcgtgc ttgtggcctt ggagatccca gtccagcgtg
      721 acgctttgcg cgctcttcca tgcttggctc gaaagcgtgc cgtcaatcgg aggatccgca
      781 tgcagcagcg gaactgcgag cgccgccgcc tggatgagcg cgagcaacat ggctacgggc
      841 cctccgcgcc gtccagattc gcgtggccga gattgcgcag cgtcgccgcg tcgagcgcag
      901 tgcaccgtcc agctcggccg cgtccggcgt aatcgcaaat ctgcgcgccg cggaaattcg
      961 caccgtgcag atccgcgccc gagaaatcgc tgcagtgcag gcggccataa cgaacggcgc
     1021 cgtcgtcgtc gacgctgtcg tcgtgagcgc agagcgcggc accctggaag ttcacgttgc
     1081 gcaggttcgc gccgttaaag tcgtcggcga cgagccgcgc gttctgcagc gacgcgccgg
     1141 aaagattcga gtcgttgaag tcgctgccga ccacctcgac gccggcgaga t
//
LOCUS       DASUCJ010000264         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1317033, whole genome shotgun sequence.
ACCESSION   DASUCJ010000264 DASUCJ010000000
VERSION     DASUCJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317033"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1243)
                     /locus_tag="VFU90_03235"
     CDS             complement(<1..>1243)
                     /locus_tag="VFU90_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478815.1"
                     /translation="DRYQYQLTSSPEYWTKCPTLGTEAKPLTVTGAADEGYYVKTLGA
                     TGKTCESGKQATILETGGTAKGTFRILSTGTVKEGKNTVTRRIVATFAHPGFTKYVYE
                     SNYEVEDPVNFGLAPSVCEYYYAERVAKGLTTKCPPIEFAPEDKVNGSMHTNDAAEVC
                     TSGGSAPTFGRTSSDEIEMNGGHYAAGGCSNTPNIVGKYTESAGSVLPPATDAELLEA
                     AGLKYEGRTEIELKSGTPNMMSVSKPGEPAKTVPYPSNGVLYVQNSKTVPCAVTNYTP
                     FGSDTVNDTGCGNVYVHGKYTESLTIASADDVIVNGSLTTTTEPNGEPNGAATLGLIA
                     ENFVRVYHPVKTRYETANVVPATQSPINGKCVTVKEVNGKIVRSNEVTELTAGGFSSG
                     DEIEGTAGQLEGGTTISETKEL"
BASE COUNT          153 a          374 c          401 g          315 t
ORIGIN      
        1 ccaattcttt ggtttccgag atcgtcgtgc ctccttcgag ctgaccggca gtcccttcga
       61 tttcgtcgcc cgaagagaat ccgcccgccg tgagttcggt gacttcgttc gacctgacga
      121 tcttgccgtt gacttctttt acggtgacgc atttgccatt gatcggactc tgcgtggcgg
      181 gcacgacgtt cgccgtttcg tatcgggttt tgaccggatg gtagacgcgg acgaagtttt
      241 cggcgatcag tccgagggtg gctgcgccgt tgggttcgcc gttgggttcg gtcgtcgtcg
      301 tcaggctgcc gttgacgatc acgtcgtcgg ccgaggcgat cgtcagcgat tcggtgtatt
      361 taccgtggac gtagacgttg ccgcaacccg tgtcgttgac cgtgtcgctc ccgaacgggg
      421 tgtagttggt cactgcgcat ggcacggtct tcgagttttg gacgtagagg acgccgttgc
      481 tcggatacgg caccgttttg gccggttcgc ccggtttcga gaccgacatc atgttcggcg
      541 tgcccgattt cagttcgatt tccgtgcgcc cttcgtactt caggccggcg gcttcgagca
      601 gttcggcgtc ggtcgccggc ggcagcaccg atcccgccga ttcggtgtat ttgccgacga
      661 tgttcggcgt gttcgagcac ccgccggcgg cgtagtgccc gccgttcatc tcgatttcgt
      721 cgctgctcgt gcgtccgaac gtcggcgcgc tgccgccgct cgtgcacacc tcggcggcgt
      781 cgttcgtgtg catcgacccg ttgactttgt cttcgggcgc gaactcgatc ggcgggcatt
      841 tcgtcgtcaa gcctttcgcg acgcgttcgg cgtaatagta ttcgcagacg ctcggcgcga
      901 gtccgaagtt gacggggtcc tcgacctcgt agttgctctc gtagacgtac ttggtgaaac
      961 ccgggtgcgc gaaggtcgcg acgattctgc gcgtgaccgt gttcttccct tctttgacgg
     1021 tgccggtcga gaggatccgg aacgtgcctt tggcggtgcc gcccgtctcg aggatcgtcg
     1081 cctgtttgcc gctttcgcag gtcttgcccg tcgcgccgag cgtcttcacg tagtagccct
     1141 cgtcggccgc gcccgtcacc gtcagcggtt tcgcttcggt gccgagcgtc gggcatttgg
     1201 tccagtattc aggcgagctc gtcagctggt actggtagcg gtc
//
LOCUS       DASUCJ010000265         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_950226, whole genome shotgun sequence.
ACCESSION   DASUCJ010000265 DASUCJ010000000
VERSION     DASUCJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950226"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFU90_03240"
     CDS             complement(<1..266)
                     /locus_tag="VFU90_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478816.1"
                     /translation="MKLFHNARIYSFDHASGRFVPQPSMIVDGTKIVAFGEEPAGIGV
                     ERIDLEGAAVLPAFADCHVHLTDTGYFLGARDLSGVRSYAAFAQA"
     gene            281..>1217
                     /locus_tag="VFU90_03245"
     CDS             281..>1217
                     /locus_tag="VFU90_03245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5478817.1"
                     /translation="MKAAKVLFGFCAGAAAGYAAVRAIEALREWRAPAPLLEKDPAAY
                     ARSRRALEVADTLRGLATALAVAYGPPGEAVDRATLRAPAWLRPALFASALSSLGAIA
                     DLPVSFVTDFTLERRYGLSEQKRRAWLADYARGTAVSTVFTAVVATLFGWAVRRSPRR
                     WPLLAALGTVPLLIAGNVIVPLYVMPLFNDFEPVTGALETRLRALAARFGVGDADILR
                     MDMSRQTRKANAFVAGVGGTHRIVIGDTLIGAFPEDEVEFVVAHEIGHYVNRDTWRLI
                     AAGEALAVALFLFANAAVAEDERAELRDRPLLIARL"
BASE COUNT          182 a          415 c          404 g          216 t
ORIGIN      
        1 gcttgcgcaa acgccgcgta cgagcgtacg ccgctgagat cccgcgcccc gagaaaatag
       61 ccggtatcgg tgaggtgcac gtggcagtcg gcaaacgccg gcaaaacggc cgcgccttcg
      121 agatcgatcc gctccacgcc gattccggcc ggttcttcgc cgaatgcaac gatcttcgtt
      181 ccgtccacga tcatcgaagg ctgcggaacg aatcggccgc tggcgtggtc gaaagagtag
      241 atacgcgcgt tatggaacag cttcacgctc gaggaatacg atgaaagccg ccaaggttct
      301 tttcgggttt tgcgccggcg cggccgccgg ctacgccgcg gtgcgggcga tcgaagcgct
      361 gcgcgaatgg cgcgcgcccg cacccctact cgagaaagat cccgctgcat atgcgcgttc
      421 tcgtcgcgcg ctcgaagtcg cggacacgct gcgcgggctc gccaccgccc tagccgtcgc
      481 ctacggtccg cccggcgaag ccgtcgatcg cgcgaccctg cgcgcgccgg cctggctgcg
      541 cccggcgctg tttgcgagcg cgctttcgtc gttaggggcc attgccgact tgcccgtttc
      601 gttcgtcacc gacttcacgc tcgaacggag atacggcttg agcgaacaga agcggcgagc
      661 ctggctcgcc gactacgctc gtgggacggc cgtctcgacc gtctttacgg ccgtcgtcgc
      721 gacgctcttc ggttgggcgg ttcggcgctc gccgcgccgc tggccgctgc tcgccgcgct
      781 cggtacggtt ccgctgctga tcgcgggcaa cgtgatcgtt ccgctgtacg tgatgccgct
      841 gttcaacgac ttcgagccgg tgacgggcgc gctcgagacc cgtctgcgcg cgctcgccgc
      901 caggttcggg gtcggcgacg ccgacatttt acgcatggac atgagccggc agacgcgcaa
      961 agccaacgcc ttcgtcgccg gcgtcggcgg tacgcaccgg atcgtgatcg gcgacacgct
     1021 gatcggcgca tttcccgaag acgaggtcga gttcgtggtc gcacacgaga tcgggcatta
     1081 cgtcaaccgc gacacgtggc gtttgatcgc ggccggcgaa gcgctggcgg ttgcgctgtt
     1141 cctgttcgcc aacgccgccg ttgcggaaga cgagcgcgcc gagctgcgcg atcgcccgtt
     1201 gctgatcgcc cgcctct
//
LOCUS       DASUCJ010000266         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_553005, whole genome shotgun sequence.
ACCESSION   DASUCJ010000266 DASUCJ010000000
VERSION     DASUCJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553005"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFU90_03250"
     CDS             <1..874
                     /locus_tag="VFU90_03250"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5478818.1"
                     /translation="FVVSVILQLADEGKLKLDDPISRFAIGVKVPNAQNITIRQLCDM
                     RSGLFEAFDTPQFNRLKVTPQMVFNPRTVIGWAVAQKPYFPPGKGYHYSNTGYLILGL
                     IIEGVTKDSVGAEIRKRLLVPFGLTHTSYPDTMAMPDPWAHGYGLDAHKNWEDVSNTI
                     PVSMMGAAGLMISNMADMKRWITLYVTGKTSKPATYKALMNCIPVAGNTSFGLGLGCS
                     ARWYGYTGGLPGYNTGEYYFMPTGATVVAWVDVQNGTPPPGVANTIFRDIARILTPNY
                     VPFQVKGGEKKSGL"
     gene            complement(895..1050)
                     /locus_tag="VFU90_03255"
     CDS             complement(895..1050)
                     /locus_tag="VFU90_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478819.1"
                     /translation="MTEKQTTAKREDDGRVSAYEIWRSRLIVVPAGDEAAFCAGWQLA
                     KDKFQQN"
     gene            1321..>1962
                     /locus_tag="VFU90_03260"
     CDS             1321..>1962
                     /locus_tag="VFU90_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5478820.1"
                     /translation="MPVQTIVVAEDDGAIRDLVAHHLEREGFTVVGAGDGQTALRRAR
                     GLADLLILDIGLPGMDGYEVARALQREQRPPPIVMLTARTDEIDRVVGFELGVDDYVC
                     KPFSPRELVARVKAILRRAGMPAAPPRPALRFGRLEIDERAREVRVDGADVRLKPREF
                     ALLLELASNPNVALSRERIIQKVWGFDFAGDERTVDVHVRRLRAKIEEPWDLPA"
BASE COUNT          361 a          613 c          644 g          344 t
ORIGIN      
        1 gttcgtcgtg agcgtcatcc tgcagctggc ggacgaaggt aaactcaagc tcgacgaccc
       61 gatcagccgg tttgcgatcg gcgtcaaggt tcccaacgcc cagaacatca cgatccgcca
      121 gctgtgcgac atgcgcagcg gacttttcga agccttcgac acgccgcagt tcaatcgtct
      181 gaaggtgacg ccgcagatgg ttttcaaccc gcgcaccgtc atcggctggg ccgtcgcgca
      241 gaagccgtac tttccgccgg gtaaaggcta ccactacagc aacaccggct atctgattct
      301 cgggttgatc atcgaggggg tcaccaaaga cagcgtcggt gcggaaattc gcaagcggct
      361 gctggtcccg ttcgggttga cgcatactag ctatcccgac acgatggcga tgcccgatcc
      421 gtgggcgcac ggctacgggc tcgacgcgca caagaactgg gaagacgtca gcaatacgat
      481 tccggtgtcg atgatgggcg cggccgggct gatgatctcg aacatggccg acatgaagcg
      541 ctggatcacg ctctacgtca ccggaaaaac gagcaaaccg gcgacctaca aagccttgat
      601 gaactgcatc ccggtggccg gaaacacgag cttcgggctc ggactcggtt gcagcgctcg
      661 ctggtacggc tacaccggcg gcctgccggg atataacacc ggggagtact acttcatgcc
      721 gacaggcgcc acggtcgtgg cgtgggtcga cgtacagaac ggcaccccgc cgccgggcgt
      781 cgccaacacc atcttccgcg acatcgcgcg catactcacg cccaactacg ttccgttcca
      841 ggtcaagggc ggcgagaaga agtcggggct gtagcacgca ccgcgcaagc ggagctagtt
      901 ttgctgaaac ttgtccttgg ctagctgcca gccggcgcag aaggcggctt cgtcacccgc
      961 cggtacgacg atgagccgcg accgccagat ttcgtaagcg ctcacgcggc catcgtcctc
     1021 gcgtttggcc gtcgtctgtt tctccgtcac ggtctcatca acggggtttt gcgcttgcga
     1081 agttcctact cttaagaaca tgacgaatgt cgtacgactt tggtcggcaa gatcgcaaag
     1141 ttctcgacgt ggagaaaatg ccgcgggcgc aatcgcaccg ttcgaactcg acggcagcct
     1201 gatgttgccg ctgtatccgc gcaaagagtt ggcgaattat gcgaacctcg gcgctgggag
     1261 atcatctgga atccggacta gcgcggctcg ccgcgcccgg cgccgggtac tggccgcgat
     1321 atgccggtgc agacgatcgt cgtcgccgag gacgacggcg cgattcgcga cctggtcgcg
     1381 caccatctcg aacgtgaggg gttcacggtg gtcggcgccg gcgacggaca gaccgcgctg
     1441 cgacgcgcgc gcggactcgc ggatctcctg atcctcgaca tcggccttcc cggaatggac
     1501 ggctacgagg tggcgcgggc gctgcagcgc gagcagcggc cgccgccgat cgtgatgctg
     1561 acggcgcgca ccgacgagat cgaccgcgtc gtcggcttcg agctgggcgt cgacgactac
     1621 gtctgcaaac cgttttctcc ccgcgaactg gtggcgcgcg tgaaggcgat tctgcgccgc
     1681 gcggggatgc cggcggcgcc gccgcggccg gcgttgcgat tcggccgctt ggagatcgac
     1741 gagcgcgcgc gggaggtgcg ggtcgacggc gccgacgtgc gtttgaagcc gcgcgagttc
     1801 gcgctgctgc tggagctggc cagtaatccc aacgtcgcgc tctcgcgcga acggatcatt
     1861 caaaaggtgt ggggattcga ttttgccggc gacgagcgga ccgtcgacgt gcacgttcgc
     1921 cgcttgcgcg cgaagatcga agaaccgtgg gatctgccgg cg
//
LOCUS       DASUCJ010000267         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1195055, whole genome shotgun sequence.
ACCESSION   DASUCJ010000267 DASUCJ010000000
VERSION     DASUCJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195055"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="VFU90_03265"
     CDS             <1..>1169
                     /locus_tag="VFU90_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5478821.1"
                     /translation="GQQNLAEDWERDISGKKPVGAEKFVVGRDVAVTPGKVIYRNRLI
                     ELIQYAPATGKVRPEPVLVVPAWIMKYYILDLSPRSSLVKYLVDQGFTVFMISWKNPG
                     PDDRDLAFDDYRALGVMAALDAVSTIVPQRAVHGVGYCLGGTLLAIAAAAMARDDDNR
                     LATLTFFAAQFDFTEAGELTLFINESQIAFLEDTMWEQGFLDAREMAGAFALLRSNDL
                     VWSRMVRAYLIGERAPMTDLMAWNADATRMPYRMHTEYLRDLFLGNDLAEGRFEAGGR
                     PVALTDIRAPVFAVATQTDHVAPWRSVYKTHLLLDTEVTFLLTSGGHNAGVVSEPGHP
                     GRVYRIATRPAEGRYVDPDHWVETARPGEGSWWPAWVAWLSSHSGSPATPPALGN"
BASE COUNT          192 a          406 c          372 g          199 t
ORIGIN      
        1 acggccagca aaatctggcc gaggactggg aacgcgacat cagcggcaaa aagccggtcg
       61 gcgccgaaaa gttcgtggtc ggccgcgacg ttgccgtcac cccgggcaag gtaatctatc
      121 gcaaccgtct catcgagctc atccaatacg cgccggcgac cggcaaggtc cggccggaac
      181 ccgttctcgt cgttcccgcc tggatcatga agtactacat cctcgacctg tcgccgcgca
      241 gctcgctcgt gaagtatctg gtcgaccagg gcttcacggt gttcatgatc tcctggaaga
      301 atcccggccc cgacgaccgc gacctcgctt tcgacgacta tcgcgcactc ggcgtgatgg
      361 ccgctctcga cgccgtgtcg acgatcgtcc cgcagcgggc cgtgcacggc gtcggctact
      421 gcctcggcgg cacgctgctc gcgattgccg ccgccgcgat ggcgcgcgac gacgacaacc
      481 gccttgcaac gctcacgttc ttcgccgcgc agttcgactt caccgaggcc ggcgagctca
      541 cgctgttcat caacgaaagc cagatcgcgt tcctcgaaga tacgatgtgg gagcagggct
      601 tcctcgacgc gcgcgagatg gccggtgcgt ttgcgctgct gcgctccaac gatctcgtgt
      661 ggtcgcgcat ggttcgcgca tacctgatcg gcgagcgcgc accgatgacc gatctcatgg
      721 cgtggaacgc cgatgcgaca cggatgccgt accggatgca cacggaatac ctgcgcgacc
      781 tcttcctcgg caacgatctc gccgaaggcc ggttcgaggc cggcggacgg ccggtcgcgc
      841 tcacggacat tcgcgcgccg gtgttcgcgg tggcgacgca aaccgaccat gtcgcgccgt
      901 ggcgctccgt ctacaagacg cacctgctgc tcgacaccga ggtgacattc ctgctcacga
      961 gcggcggaca caacgccggc gtcgtgtcgg agcccggtca tccgggtcgc gtctatcgca
     1021 tcgcaacgcg gccggccgaa ggccgctacg tcgacccgga ccattgggtc gaaacggcgc
     1081 gtccgggtga aggctcgtgg tggccggcgt gggtcgcctg gctttcctcg cattcgggtt
     1141 ccccggccac gccgcccgcg ttgggcaac
//
LOCUS       DASUCJ010000268         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_384759, whole genome shotgun sequence.
ACCESSION   DASUCJ010000268 DASUCJ010000000
VERSION     DASUCJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384759"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1002
                     /locus_tag="VFU90_03270"
     CDS             <1..>1002
                     /locus_tag="VFU90_03270"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU5478822.1"
                     /translation="DNAVEYFVSYYDYYQPEAYIPRTDTYIEKDSSINEQIDRMRHAA
                     TRALLERDDVIIVASVSCIYGIGSVETYSAMTFSLKQGERLDQRALLADLVALQYKRT
                     AGDFYRGSFRVRGDAIEIFPAHYEDRAWRVSLFGDEVESIHEFDPLTGQKTDELEFVK
                     IYANSHYVTPRPTLLQAIAGIKLELRARLDQLNAAGRLLEAQRLEQRTLYDLEMMEAT
                     GACAGIENYSRYLTGRKPGEPPPTLFEYVPDNALVFVDESHVTVPQLGGMYRGDFRRK
                     ATLAEYGFRLPSCMDNRPLRFEEWDAMRPQTVCVSATPSGWELNESGGVFTEQVIRPT
                     "
BASE COUNT          193 a          326 c          306 g          177 t
ORIGIN      
        1 cgacaacgcg gtcgaatatt tcgtcagtta ttacgactac taccagccgg aagcctacat
       61 cccgcgcacc gacacctata tcgagaagga ctcctcgatc aacgagcaga tcgaccgcat
      121 gcgccacgcg gcgacgcggg cgctgctcga gcgcgacgac gtgatcatcg tcgcctcggt
      181 gtcgtgcatc tacggtatcg gctcggtcga gacctattcg gcgatgacgt tttcgctcaa
      241 gcaaggcgag cggctcgatc agcgtgcctt actcgccgat ctggtcgcgt tgcaatacaa
      301 acgcaccgcc ggcgatttct atcgcggctc gttccgggtg cgcggcgatg cgatcgagat
      361 tttcccggcg cactatgagg atcgcgcctg gcgcgtgtcg ctgttcggcg acgaagtgga
      421 atcgatccac gaattcgatc cgctcaccgg ccagaagacc gacgaactgg aattcgtgaa
      481 aatctacgcc aactcccact atgtgacacc gcggccgacg ctgttgcagg cgatcgccgg
      541 catcaagctc gaattgcgcg cgcggctcga tcagctcaat gccgccgggc gtttactgga
      601 agcgcagcgg ctcgaacagc gcacgctcta cgatctggaa atgatggagg cgaccggcgc
      661 ctgcgccggc atcgagaact attcgcgcta tctcaccggg cgaaagcccg gcgagccgcc
      721 gccgacattg ttcgaatatg tgcccgacaa tgcgctggtg ttcgtcgacg aaagccacgt
      781 caccgtgccg cagctcggcg gcatgtatcg cggcgacttc cggcgcaagg cgacgctcgc
      841 cgaatacggc ttccgcctgc cctcgtgcat ggacaaccgg ccgctgcgct tcgaggagtg
      901 ggacgccatg cggccgcaaa cggtctgcgt atcggcgacg cccagcggct gggagctcaa
      961 cgaatccggc ggcgtgttca ccgagcaggt gatccgcccg ac
//
LOCUS       DASUCJ010000269         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_598760, whole genome shotgun sequence.
ACCESSION   DASUCJ010000269 DASUCJ010000000
VERSION     DASUCJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598760"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..>1182)
                     /locus_tag="VFU90_03275"
     CDS             complement(233..>1182)
                     /locus_tag="VFU90_03275"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5478823.1"
                     /translation="FNKAQQDAVLPYVEDGTVTLIGATTENPSFEVNSALLSRARVFV
                     LKQLSDEDVARIVERALADPQRGLGKLNAGLAGDARATLVGLANGDARAALNALEFAA
                     VSAPDSGGRREISKALVIDAMQRRGTLYDKGGEAHYDTVSAFIKSIRASDPDAAIYWL
                     ARMLDGGEDPLFVARRLVILASEDVGLADSHGLTIAVAAQQAVHFVGMPEGFYALAHA
                     TLYLARAPKSNSVGRAYSAALADVLETRNDPVPLHLRNAPTGLMRGLGYGKGYHYAHD
                     DYDVVQQNLPDNLAGRRYYRPGDRDEGRAAGDDKKRRSE"
BASE COUNT          189 a          387 c          390 g          216 t
ORIGIN      
        1 cgcctccctt cgaggtgcag cgaactcgga ttgtaaccgc cgatttcgtc ggcggcgcgc
       61 atcgcctcgg cgctttcccg gcgcagcggc gtcgtcgccg cgtagtcgag ataaatgcgc
      121 tccgttcgcc ggttcacagg acgctcccag tgtgccgttc tatcataatt ttcgtgcgaa
      181 gctccaacgc ataactccga gaagtagcaa cgggaaaggg gcggttcgag tactactcgg
      241 accgcctttt tttatcgtcg ccggctgcgc gcccttcgtc gcgatcgccc ggacgatagt
      301 aacgccggcc ggcgagattg tcgggcagat tctgctgcac gacgtcgtaa tcgtcgtgtg
      361 cgtagtggta gcctttcccg taacccaagc cgcgcatcag gccggtcggc gcgttgcgca
      421 ggtgcagcgg gaccgggtcg ttgcgcgttt cgagcacgtc ggcgagtgcc gccgagtagg
      481 cgcgtccgac gctgttgctc ttgggagcgc gcgctaagta aagcgtcgcg tgcgcgagcg
      541 cgtagaagcc ttcgggcatc ccgacgaagt gcacggcttg ctgggcggcg acggcgatcg
      601 tcagcccgtg cgagtcggcc agcccgacgt cctcggatgc gagaatcacg agccggcgtg
      661 ccacgaagag cgggtcttcg ccgccgtcga gcatacgggc cagccagtag atcgcggcat
      721 cggggtcgct cgcacgaatc gatttgatga acgcgctgac ggtgtcgtaa tgcgcctcgc
      781 cgcctttgtc gtagagcgtc ccgcgccgct gcatcgcatc gatcacgagc gctttggaga
      841 tctcgcgccg gccgcccgaa tccggcgccg agacggccgc aaattccagc gcgttgagcg
      901 cggcgcgggc gtcgccgttg gccaagccga cgagggtggc gcgagcgtcg ccggccagcc
      961 cggcattcaa ctttcccaag cctcgttgcg gatccgcgag cgcccgctcg acgattcgcg
     1021 cgacgtcttc gtcggacaac tgcttcaaaa cgaagacgcg cgcccgcgac agcagcgccg
     1081 agttcacctc gaacgacggg ttttcggtgg tcgcgccgat cagcgtgacc gtaccgtcct
     1141 cgacgtacgg caagaccgcg tcttgctgcg ccttgttgaa ac
//
LOCUS       DASUCJ010000270         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1301795, whole genome shotgun sequence.
ACCESSION   DASUCJ010000270 DASUCJ010000000
VERSION     DASUCJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301795"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFU90_03280"
     CDS             <1..258
                     /locus_tag="VFU90_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5478824.1"
                     /translation="GLSWPSGLVAAGGWIAGALLTQYSSVGSMLGSVLAPVALWAFTR
                     SPAETLYGILAAIFIVYTHRENLVRLRAGTEGPIRLLRRKP"
     gene            308..868
                     /gene="efp"
                     /locus_tag="VFU90_03285"
     CDS             308..868
                     /gene="efp"
                     /locus_tag="VFU90_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5478825.1"
                     /translation="MISSNDLRNGITIVIDGQLWTVIEFLHVKPGKGSAFVRTRIKNV
                     RNGTTLERTFRAGEKLERATVDNRQMQMLYNDADGYHFMDQETFENVSIGRDLIGDPA
                     DFLKDGMVVDVQFHDGTPIGVDLPAHVELKIVETDPGFRGDTATNTTKPAKLETGATV
                     QVPLFVETGNVIRIDTRDRRYIGRSS"
     gene            882..2771
                     /locus_tag="VFU90_03290"
     CDS             882..2771
                     /locus_tag="VFU90_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5478826.1"
                     /translation="MRARLRALVVALVLGAVASAAGAILTALPLTTLPALNRIADLSV
                     HVGALDEAGRLGLGKPAYESEPNPNLAIVTIDDAAYHKLGFPFPRRYYATVLRNLRAA
                     GARAVAFDIEFLEPSKDSAQDAAFAAALRGVPSVLAYSIDTTSTGRIGEELPIPRLAE
                     AARRVGYLTVDSPGGFVIGQPMEIATAGTGEHANERLLSLAAAAVTTFRGAPLNLAAI
                     PTDADGRMLLLPPKLEHHQDLASGTDVQTQAFAGRGTMPFDGAMTSSPQDLRAFANGA
                     LVFVGATAQGAFDYSTSAGRGRIPGLFVNARFADQLMRGYYLRPAPLGFDIALAVLLP
                     LLSVLGFTLMRTTYAILAAVAATLVYAYFNLWLFVERLYWLDLVHVVLAMLLGTLFVA
                     VYRTLYEASQRRMVTNLFGMHVSPAVVSDILAHDDPRQALALRGKRVTATIFYSDIRG
                     FTAMSETMTPEEIYTQLNEYFEEMCKIIFAYGGYVDKFIGDCVMAVFSAPYQTPADAR
                     NAVVCAVKQQERIRELSGRWKAEGKREFSVGMGINTGDVVMGNLGASSRMNYTVIGDD
                     VNVAARLYNVAAGGEIIISETTYALCRDAVDVEALEPVAVKGKSRPIAIYRVTALRAG
                     AAPSA"
     gene            2779..>3002
                     /locus_tag="VFU90_03295"
     CDS             2779..>3002
                     /locus_tag="VFU90_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5478827.1"
                     /translation="MTGALEIFALALFASAFGSMVGLGGGFILVPALRLFFGLPPAEA
                     AGTSLILIVANSASGAFTYLLQRRVHLKIG"
BASE COUNT          506 a         1014 c          945 g          537 t
ORIGIN      
        1 gggctcagct ggccatccgg actggtggcc gccggcggct ggatcgcggg cgcgctgctc
       61 acgcagtatt cgtcggtcgg ctcgatgctc ggcagcgttc tcgcgccggt cgcactgtgg
      121 gcgttcaccc gctcgccggc cgagacgctc tacggaattc tcgcagcgat cttcatcgtc
      181 tacacgcatc gcgagaatct cgtccgcttg cgggcgggca ccgaggggcc gatccggctg
      241 ttgcgacgta agccgtaggc tgccaagcca gggcttgcgc gttggaggcc cgtaatggcg
      301 gaatgtcatg atctcgtcga acgacctccg taacggcatt accatcgtca tcgacggcca
      361 gctttggacc gtcatcgagt ttctgcacgt caagcccggc aagggatcgg cgttcgtacg
      421 cacgcggatc aagaacgttc gcaacggaac caccctcgaa cggacctttc gcgcgggcga
      481 gaagctcgag cgcgcgacgg tcgacaaccg gcaaatgcaa atgctctaca acgatgccga
      541 cggctaccac ttcatggacc aggagacgtt tgagaacgtt tcgatcggcc gcgacttgat
      601 cggtgacccc gccgactttc tcaaagatgg aatggtcgtc gacgtgcagt tccacgacgg
      661 cactccgata ggcgtcgact tgccggcgca cgtcgagctg aagatcgtcg aaaccgaccc
      721 gggattccgc ggcgatacgg ccaccaacac gacgaagccc gcgaagctcg aaacgggagc
      781 cacagtccaa gttccgctgt tcgtcgagac cggcaacgtc atacgcatcg atacgcgcga
      841 ccgccgctac atcgggcgat cgagctagcc agtagccgaa cgtgcgggct cggctgcgcg
      901 cgctggtcgt cgctctcgtc ttgggagcgg tcgcgagtgc ggctggcgcg atcctcaccg
      961 cgcttccgct cacgacgctc cccgcactca accgaatcgc cgatttgtcc gttcacgtcg
     1021 gcgcgctcga cgaggccggc cgcctgggtt taggcaagcc cgcatacgag agcgagccga
     1081 acccgaacct cgcgatcgtc accatagacg acgccgccta ccacaagctc ggcttcccgt
     1141 ttccgcgccg ctactacgcg acggttctcc ggaacctgcg cgcggcgggc gcgcgagccg
     1201 tagccttcga catcgagttt ctcgagccga gcaaggactc cgcacaggac gcggcgttcg
     1261 cggccgcgct tcgcggagtt ccttcggtgc tcgcgtattc gatcgacacg acgagcaccg
     1321 gccggatcgg agaagagctg ccgattccgc ggctcgccga ggcggcgcgc cgcgtgggat
     1381 atctcacggt cgattctccc ggcgggttcg ttatcggcca gccgatggaa atcgcgaccg
     1441 ccggcacggg agaacacgcc aacgaacgcc tgctatcgct cgctgcagcc gccgttacta
     1501 cgtttcgcgg cgcgccgttg aatctcgccg cgattcccac cgacgccgac ggccgcatgc
     1561 tgctgctccc gccgaaactc gagcaccacc aggatctcgc gagcggcacc gacgtgcaga
     1621 cgcaggcctt cgccggccgc ggaacgatgc cgtttgacgg cgcgatgacg agctcgccgc
     1681 aagacctgcg cgcgtttgcc aacggcgcgc tggtgttcgt cggcgcgacc gcacaaggcg
     1741 cgttcgacta ctcgaccagt gccgggcgcg gccggatacc gggattgttc gtcaacgccc
     1801 gctttgccga ccagctgatg cgcggatact acctgcgtcc ggcaccgctg ggtttcgaca
     1861 tcgcactcgc cgtgctgctg ccgctgctct ccgtgctcgg gtttacgctg atgcggacga
     1921 cgtacgcaat cttggcggcc gtcgccgcca ctctcgtcta tgcgtacttc aatttgtggc
     1981 tgttcgtcga gcggctctac tggctcgatc tcgttcacgt cgtgctcgcg atgctgctcg
     2041 gtacgctgtt cgtcgccgta taccgaacgc tctacgaagc gtcgcagcgg cgtatggtga
     2101 cgaatttgtt cggcatgcac gtcagtcccg cggtcgtttc ggacattcta gcgcacgacg
     2161 atccccgcca agccctggcc ctgcgcggaa aacgcgtcac ggcgacgatc ttctacagcg
     2221 acatccgcgg cttcaccgcg atgagcgaga cgatgacccc tgaagaaatc tacacgcagc
     2281 tcaacgagta cttcgaagag atgtgcaaga tcatcttcgc ctacggcgga tacgtcgaca
     2341 agttcatcgg cgactgcgtg atggccgtct tctcggctcc ctatcaaacg cccgccgacg
     2401 cgcgcaacgc ggtggtttgc gcggtgaaac agcaggagcg catccgcgag ttgagcggcc
     2461 gctggaaggc cgagggaaaa cgcgagttct cggtgggcat gggcatcaac accggcgacg
     2521 tggtgatggg aaacctcggc gcctcctcgc gcatgaacta cacggtcatc ggcgacgacg
     2581 tgaacgtcgc ggctcgtctc tacaacgtcg ccgccggcgg cgagatcatc atcagcgaga
     2641 ccacctacgc tctctgccgc gatgcggtcg acgtcgaggc gctcgagccg gtcgcggtca
     2701 aaggcaagag ccggccgatc gccatctacc gggtgacggc gctgcgcgcg ggcgcggcgc
     2761 cctcggcgta agccgcgcgt gaccggcgcg ctcgagatct tcgccctcgc gcttttcgcc
     2821 agcgctttcg gctcgatggt gggtctcggc gggggattca tcctcgtgcc cgcgttgcgg
     2881 ctgtttttcg ggctcccgcc cgccgaagcg gccggcacgt cgctgattct gatcgtcgcc
     2941 aacagcgcga gcggggcctt cacctatctt ctgcagcggc gcgtccactt gaagatcggc
     3001 tt
//
LOCUS       DASUCJ010000271         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1408830, whole genome shotgun sequence.
ACCESSION   DASUCJ010000271 DASUCJ010000000
VERSION     DASUCJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408830"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFU90_03300"
     CDS             <1..473
                     /locus_tag="VFU90_03300"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5478828.1"
                     /translation="DEMYAAARLTAGLLPVAKPRYLMGVGTVRDVVEAIDCGIDMFDC
                     VYPTRCGRNGRAMTHRGHFNIFNAAYVADFAPVDPECDCCVCRTFTRAYLAHLFRSKE
                     MLGPRLLSYHNVYLLDRLMQQARAAIAADGWQDFRARANAAQENDGPPHPPGGR"
     gene            490..1158
                     /locus_tag="VFU90_03305"
     CDS             490..1158
                     /locus_tag="VFU90_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU5478829.1"
                     /translation="MPKILCVVTSHSHIDADHPTGLWLDEYAIPYAAFRAAGFDVVTA
                     SPRGGLAPIDPRSLEGAAPGDAARAELSKTRTLQEAGDALEYDAIFIPGGHGTMFDLA
                     QSLPLKTLLSEFDVQGKLIASVCHGPAAFVDAIRAGQPATLVAGRRITCFTDAEEQAT
                     GLAKFMPYLLASKLREQGAEVVEREPWTDHVEIDGNWITGQNPQSSASAAAAVVAALG
                     VRRT"
BASE COUNT          209 a          429 c          406 g          188 t
ORIGIN      
        1 gcgacgaaat gtacgccgct gcgcggctga ccgctgggct gctgccggtc gcgaagccgc
       61 gctacctgat gggcgtagga acggtacgcg acgtcgtcga ggcgatcgac tgcgggatcg
      121 acatgttcga ctgcgtctat cccacccgct gcggccgcaa cggccgggcg atgacgcacc
      181 gcggccactt caacatcttc aacgcagctt acgtcgccga tttcgcaccg gtcgatccgg
      241 agtgcgactg ctgcgtctgc cggacgttca cgcgcgccta cctcgctcat ctgtttcgct
      301 cgaaggaaat gctgggaccg cggctgctgt cctaccacaa cgtctacctg ctcgaccgct
      361 tgatgcagca ggcgcgcgcg gccatcgcag cggacggctg gcaagacttc cgagctcgcg
      421 cgaacgccgc ccaggaaaac gacggcccgc cgcatccgcc cggcggccga tagggttcga
      481 gggaagacca tgcccaagat tctgtgcgtc gtcaccagcc actcccacat cgatgccgac
      541 catccgaccg gcctctggct ggacgaatat gcgattccgt acgcggcgtt ccgcgcggcg
      601 ggcttcgacg tcgtgacggc cagcccgcgc ggcgggctcg cgccgatcga cccgcgcagc
      661 ctcgaaggtg ccgctcccgg cgatgcggcg cgcgcggagc tctcgaaaac gcgcacgctt
      721 caggaagccg gcgatgccct cgaatacgac gcgatcttca ttccgggcgg ccacggcacg
      781 atgttcgacc tcgcgcaaag cctgccgctg aaaacgctgc tcagcgaatt cgacgttcag
      841 gggaagctga tcgcgtcggt ctgccacggg ccggccgcgt tcgtcgacgc gattcgcgcg
      901 ggtcagccgg ccacgctggt tgcgggacgg cgcatcacct gctttaccga cgccgaagag
      961 caagccaccg gcctcgcgaa attcatgccg tatctgctcg catccaagct gcgcgaacaa
     1021 ggcgccgagg tcgtcgagcg cgagccctgg acggatcacg tcgagatcga cggcaactgg
     1081 attacgggac agaacccgca gtcgagcgcg agcgcggcgg ccgcggtcgt agcggcgctg
     1141 ggagttcgcc ggacttagcc gtcgagagcg cgcaccaccg cggcgcattc ggcgcagatc
     1201 gacggatggc cgggatccgc tccgagttcg cg
//
LOCUS       DASUCJ010000272         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_874025, whole genome shotgun sequence.
ACCESSION   DASUCJ010000272 DASUCJ010000000
VERSION     DASUCJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874025"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFU90_03310"
     CDS             <1..931
                     /locus_tag="VFU90_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5478830.1"
                     /translation="IVGVKPTYGGVSRYGLVAFSSSLDQAGPFARNILDAALLHAAIA
                     GHDPLDSTSIDAPVPDVVTAARAGDIAGVRVGVVRELNGEGYDAGVRQRFSEAVSLLG
                     SLGAEVGEASCPHFDYAMPAYYLIAPSEASSNLARFDAMRYGLRVGDDGTRSAEEVMA
                     LTRAAGFGPEVKRRIMIGTYALSSGYYDAYYGSAQKVRTLISQDFAAAFEKFDVLVSP
                     STPTTAFRIGERTDDPLKMYLADLDTIPSNLAGNASMSMPVGLSPDDGLPVGLQIMAP
                     ALADDRLYRVAAALEAALSQRWGHALIEEAPAL"
     gene            928..>1390
                     /locus_tag="VFU90_03315"
     CDS             928..>1390
                     /locus_tag="VFU90_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478831.1"
                     /translation="MSTTYDEALAKYEPVFGLETHVELGTASKMFCGCGTTFGAAPNT
                     QVCPVCLGLPGSLPVANAMAIESTIRIGLALHCTIARWCRFARKNYFYPDMPKNFQTS
                     QYDEPITIDGWLDVDGTHIGITRAHLEEDTGKTTHMGGAGRIHGADYSLVDY"
BASE COUNT          227 a          452 c          461 g          250 t
ORIGIN      
        1 aatcgtcggc gtgaagccga cgtacggcgg tgtttcgcgc tacggcttgg tcgcgttctc
       61 ctcatcgctc gaccaagccg gcccgttcgc ccgcaacatc ctcgacgccg cactgctgca
      121 cgccgcgatc gccggacatg acccgcttga ctccacgtcg atcgacgcgc cagttccgga
      181 cgtcgtgacc gccgcacggg caggcgacat cgccggcgtg cgggtcggcg tcgtccgcga
      241 gttgaacggt gagggttatg acgcaggcgt gcggcaacgc ttctccgagg cggtgtcgct
      301 gctggggtcg cttggcgccg aggttggcga ggccagctgc ccgcatttcg actacgcgat
      361 gccggcgtat tacctgattg cgccgagtga ggcgtcgtcc aacctggctc gattcgacgc
      421 gatgcgttac ggcttgcgcg tcggtgacga cggcacgcgc agcgctgaag aggtgatggc
      481 gctcacgcgt gccgcggggt tcggacctga ggtgaagcgg cgcatcatga tcggcacgta
      541 cgcgctgtcg agcgggtact acgacgcgta ctacggctca gcgcagaaag tgcgcacgct
      601 gatcagccaa gatttcgctg ccgcgtttga gaaattcgac gttttggtgt cgccatcgac
      661 gccgaccacg gcgttccgca tcggcgagcg caccgatgat ccgctgaaga tgtatctcgc
      721 cgacctcgac acgattccgt ccaatcttgc gggtaacgct tcgatgtcga tgccggtcgg
      781 cctttctccc gacgacgggc tcccggttgg gctgcaaatc atggcgccgg cgcttgccga
      841 cgaccggctc taccgcgtcg ccgcggcgct cgaggcggcg ctgtcgcagc gatggggaca
      901 cgcgttgatc gaggaggctc cggcgctgtg agcaccacat atgacgaagc gctggcgaag
      961 tacgaaccgg tgttcgggct tgagacccat gtcgaactcg gcacggcgtc gaagatgttc
     1021 tgtggctgcg gcaccacgtt tggcgcggcg cccaacacgc aggtgtgccc ggtgtgcctt
     1081 ggcctgcccg gctcgctgcc ggtggccaac gcgatggcga tcgagtcgac gatccgcatc
     1141 gggctcgcgc tgcactgcac gattgctcgg tggtgccgct tcgctcgcaa gaactacttc
     1201 tatcccgaca tgccgaagaa ctttcagacc tcgcagtacg acgagccgat cacgatcgac
     1261 ggctggctcg acgtcgacgg cacgcacatc ggcatcaccc gtgcgcacct cgaagaggac
     1321 accggcaaga cgacgcacat gggcggcgcg ggccgcattc acggtgccga ctactcgctg
     1381 gtcgactaca
//
LOCUS       DASUCJ010000273         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_247395, whole genome shotgun sequence.
ACCESSION   DASUCJ010000273 DASUCJ010000000
VERSION     DASUCJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247395"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1287
                     /locus_tag="VFU90_03320"
     CDS             <1..>1287
                     /locus_tag="VFU90_03320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEU5478832.1"
                     /translation="PPAAPQTPPQGVRHALAAALAVGVPLPFQSYEFWLFVGLTLLVV
                     RLTAGRVRQLSLLAASYYFYARWNGWYVLFLLILTASDYGIALLLERVALRARKLTLS
                     LGIAANLAFLGTFKYANFASGTIAALVGMHANPWLVQLFVPIGISFHTFQSISYLVDV
                     YRGKFRAVRNPLDYALYLAFFPQLLAGPIVRAGLFFAEFFDWRMPDAADFGYGLMRIG
                     FGLVKKVAIADQFAPLSDAYFGSIGTHPGAAAAAGAIFAFSIQIYFDFSGYSDIAIGC
                     ARLFGFVFPENFIQPYLATSVTDFWHRWHVTLSTWLRDYLYIPLGGSRGGLAETIRNL
                     MLTMLLGGLWHGAQWTFVAWGAYHGVLLSIERILGIGRPEDRPSGPRYVARVAVTFVV
                     VSLGWVLFRAPGFGAAAEVYRQLAIGGWRGWPLQGW"
BASE COUNT          166 a          436 c          408 g          277 t
ORIGIN      
        1 atccgccggc cgcgccgcaa actccgccgc agggcgttcg gcacgcgctc gccgccgcgc
       61 tggccgtcgg cgtgccgctg ccgtttcaat cgtacgagtt ctggctgttc gtcggactca
      121 cgctgctcgt cgtccgcctg acggccggac gcgtgcgcca gctgtcgttg ctggcggcga
      181 gctactactt ctacgcccgc tggaacgggt ggtacgttct gttcttgctg attctcaccg
      241 cttccgatta cggcatcgcg ctgctgctcg aacgcgtcgc acttcgcgcg cgcaagctca
      301 cgctgtcgct cggcatcgcg gccaacctcg cgttcctcgg aacgttcaaa tacgcaaact
      361 ttgcaagcgg cacgatcgcg gcgctggtcg ggatgcacgc gaatccctgg ctcgtgcagc
      421 tgttcgtccc gatcggcatc agcttccaca ccttccagag catctcgtat ctggtcgacg
      481 tctatcgcgg caagttccgg gcggtacgca atccgctgga ctacgcgctc tacctcgcgt
      541 tctttccgca gctgctggcg gggccgatcg tgcgcgccgg gctcttcttc gcggaattct
      601 tcgactggcg catgcccgac gccgccgatt tcggctacgg cttgatgcgg atcgggttcg
      661 ggttggtcaa gaaggtcgcg atcgccgatc agttcgcacc gttaagcgac gcctatttcg
      721 gctcgatcgg cacccacccc ggagcggcgg cggcggccgg cgcgatcttc gcgttttcga
      781 tccagatcta tttcgatttc tccggctaca gcgacatcgc aatcggctgc gcgcggctgt
      841 tcgggttcgt ctttccggaa aacttcattc agccgtatct cgcgaccagc gtcaccgatt
      901 tttggcatcg gtggcacgtc actctgtcca cgtggctgcg cgactacctc tacattccgc
      961 tcggcggcag ccgcggcgga ctggccgaga cgatccgcaa cctgatgctc acgatgctgc
     1021 tcggcggatt gtggcacggc gcgcagtgga cgttcgtcgc ctggggcgcg tatcacggcg
     1081 tgctgttgtc gatcgagcga attctgggta tcggccgtcc ggaggatcgc cccagcggcc
     1141 cccgttacgt cgcccgcgtg gcggtaacgt tcgtcgtggt gtcgttgggc tgggtgctct
     1201 tccgcgctcc cggattcggc gccgccgccg aagtctaccg gcagctcgcg atcggcggct
     1261 ggcgaggctg gccgctgcag ggctggc
//
LOCUS       DASUCJ010000274         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1026996, whole genome shotgun sequence.
ACCESSION   DASUCJ010000274 DASUCJ010000000
VERSION     DASUCJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026996"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFU90_03325"
     CDS             complement(<1..250)
                     /locus_tag="VFU90_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478833.1"
                     /translation="MPRVLATAATLVALGLGTPARGAGGADERAAGAGDRLGAAVARA
                     AAAIAVGTGLGAGSAGLARAVGAAVGAGGLGERTAVATA"
     gene            222..815
                     /locus_tag="VFU90_03330"
     CDS             222..815
                     /locus_tag="VFU90_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478834.1"
                     /translation="MAAVAKTRGTAPSPGPKGEGSPGPRAGTSAKTQAAPARPIQLRP
                     TPSPAPHAKPAAPDINARLRAMLPHNPVNPASKQYTPQLSLRGSLEPTPPPSVLAQTK
                     YIYRSSGGSEARVEMWVTEVRKAGPTTICTGWLVRYPYNQTASHPGDFAPANGTQVSI
                     GGGRVPAVLPPVVEGIVTLACEGRLLVPYAASPAASP"
     gene            1140..1667
                     /locus_tag="VFU90_03335"
     CDS             1140..1667
                     /locus_tag="VFU90_03335"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478835.1"
                     /translation="MVATDIAVAFERELDAIAHDAIAFPELEILHRRTRAGRGTTAFG
                     VTIDRPGGVDLALCERVAARLNLALETFGDHYSLEVESAGLERPLVRPGDYTRFSGQP
                     VRVVTTLTVNGGKTHRGILRGLRGESVVLETAGGELPLPLPTIKSAHLEYDPRADLQR
                     NKQQRKKSHDDRHRI"
     gene            1645..2859
                     /gene="nusA"
                     /locus_tag="VFU90_03340"
     CDS             1645..2859
                     /gene="nusA"
                     /locus_tag="VFU90_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5478836.1"
                     /translation="MTTDTVSDEKLIDVLHSIAKERNISFEMLLEALEAALLTAYKRH
                     FGSESNAIVTVDRQNGAYKVYHRRTIVDEVVDPKLEISVKAAGGPYQAGDFYDEEVTP
                     KDFGRIAAQTAKQVIVQRIREAERDTVYNKYVRKLNDLVTGTVQRYEQRNMYVTLEGR
                     DEAVMYLDEQVPGEAYRINDFIRAYVLDVKKSPKGPQVILSRAAEGLVQRLLELEVPE
                     IADGTVEIMAIARDPGSRSKVAVKSNRAEVDPIGACLGPKSSRIANVTDNLRGEKIDI
                     IPYAADPATFIMKALAPAKVIGVELFEDDGVALVIVPDYQLSLAIGRDGQNVRLAARL
                     TGWRLDITSEAESNEARERYLAERAERTTDEGAEEVAEPAVALEGADIDPDLIRKLEE
                     FRRERLGAEGEE"
     gene            3243..>3550
                     /locus_tag="VFU90_03345"
     CDS             3243..>3550
                     /locus_tag="VFU90_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5478837.1"
                     /translation="MATGKVRIFELAKEVGLPSKELISLFNERLGGVFEAKNQLSVVP
                     DQIADLIRSVLLKSGAAPAKKPERAAPAAPAKAKAAPAAPAKRAAPRPAAPPAPEAPV
                     P"
BASE COUNT          664 a         1185 c         1196 g          505 t
ORIGIN      
        1 ccgcggtggc gacggccgta cgctcgccca aaccgccggc gccgacggcg gcgccgaccg
       61 cgcgcgcgag ccccgcactc ccggccccca gcccggttcc gaccgcaatt gccgcggcgg
      121 cgcgggccac cgccgccccc agcctgtcgc cggcaccggc cgcccgttcg tcggcgccgc
      181 cggcaccgcg ggccggcgtc ccgagcccca gcgcgaccag cgtggcggcg gtcgccaaga
      241 cgcgcggcac ggctccgagc ccaggcccga aaggcgaagg ctcccccggc ccgcgcgccg
      301 gcacgagcgc gaagacgcaa gcggcaccgg cgcgcccgat ccagctgcgc ccgaccccca
      361 gcccggcgcc gcacgccaag ccggccgcgc ccgacatcaa cgcgcggctg cgggcgatgc
      421 ttccccacaa tccggtcaat ccggccagca agcagtacac gccgcagctc tcgctgcgcg
      481 gctcgctcga accgacgccg ccgccgagcg tgctggcgca gacgaaatac atctaccgct
      541 cgagcggcgg cagcgaagcg cgcgtcgaga tgtgggtgac cgaggtgcgt aaggccgggc
      601 cgacgacgat ttgcaccggc tggctcgttc gctacccgta caatcaaacg gcgtcgcatc
      661 cgggcgattt cgcgccggcg aacgggacgc aggtttcgat cggcggcggg cgggtgccgg
      721 ccgtcctccc cccggtggtc gaagggatcg tgacgctggc gtgcgagggg cgcttgctcg
      781 tgccgtacgc cgcgtcgcct gctgcgtcgc cgtaacgagg gtgcattccc gcttgagttc
      841 cggcggcagt cgcaatagaa ttacatcacc gaattcggca cacttcgtct aacgaaggag
      901 ggcggttgga accgcaagat ccaccgggct gtggagcccg cgacagacgc gccggataac
      961 gggcagcgga gaacgcgccc acgttgaccg gacgattacc ggaggcgcgc gatcgcataa
     1021 caaagagaca aagtgagggg ccgcgcccac gtggggttgc ggcccgtcgc tgttttcgct
     1081 attatgtagg cggtttcgca ggagcgtggg caggtcccgc gcttcgttgt ttcgagggta
     1141 tggtggctac tgacatcgct gtggcgttcg agcgcgagct ggacgccatc gcacacgacg
     1201 caatcgcctt tccagagctg gagatcctgc accgccggac gcgcgccggc cgcgggacga
     1261 cggcgttcgg cgtaacgatc gatcgcccgg ggggcgtcga tttggcgttg tgcgaacgcg
     1321 ttgccgcccg gctcaacctg gcgctcgaaa ccttcggcga ccactattcg ctcgaggtag
     1381 aatcggcggg gctcgagcgt ccgctcgtgc ggccgggcga ctacacgcgc ttcagcggtc
     1441 aacccgtccg cgtcgtcacg acgttgacgg tcaatggcgg gaagacgcat cgcggcatct
     1501 tgcgcggcct gcgcggcgag tcggtcgtac tcgaaacggc cggcggcgag ttaccgctgc
     1561 ccttgccgac catcaagtcg gcgcatctcg aatacgatcc gcgcgcggat ctgcaacgaa
     1621 acaaacaaca acgaaagaaa tcccatgacg acagacaccg tatctgacga aaaactcatc
     1681 gacgtcctgc actccatcgc caaggagcgc aacatctcgt tcgagatgct gctcgaagcg
     1741 ttggaagccg ctctgctgac ggcctacaaa cgccacttcg gcagcgaatc caacgcgatc
     1801 gtgacggtcg atcgccaaaa cggtgcctac aaggtgtacc accgccggac gatcgtcgac
     1861 gaagtcgtcg atccaaagct cgaaatctcg gtcaaagcgg ccggcggccc gtaccaggcg
     1921 ggcgacttct acgacgaaga ggtgacgccc aaagatttcg gacggatcgc cgcgcagacg
     1981 gccaaacagg tcatcgtaca gcgcattcgc gaggccgagc gcgacacggt ttacaacaag
     2041 tacgtccgca agctcaacga cctcgtgacc ggcacggtgc agcggtacga acagcgcaac
     2101 atgtacgtga cgctcgaagg ccgcgacgag gcggtgatgt atctcgacga acaggtaccg
     2161 ggcgaagcct accgcatcaa cgacttcatt cgcgcgtacg tcctggacgt caagaaatcg
     2221 cccaaagggc cgcaggtgat tctgtcgcgc gcggccgagg ggctcgtgca gcggctgctc
     2281 gagctggagg ttccggagat cgccgacggt accgtcgaga tcatggcgat cgcgcgcgac
     2341 cccggcagcc gctcgaaagt cgcggtcaag agcaatcgcg cggaagtcga tccgatcggc
     2401 gcctgtctcg gaccgaagtc gagccggatc gcaaacgtca ccgacaacct gcgcggcgaa
     2461 aaaatcgaca tcattcccta cgccgcggat cccgccacgt tcatcatgaa agcgctggcg
     2521 ccggcgaaag tcatcggcgt ggagctgttc gaggacgacg gcgtggcgct ggtaatcgtg
     2581 cccgattacc aactgtcgct cgcgatcggg cgcgacggcc agaacgtgcg gctggcggcg
     2641 cggcttacgg ggtggcggct cgacatcacg agcgaagccg aatcgaacga agcgcgcgag
     2701 cgttatttgg ccgagcgcgc ggaacggacc accgacgagg gggccgagga ggtcgccgag
     2761 ccggccgtcg cgctggaagg cgccgatatc gatccggacc tgattcgcaa gctcgaggag
     2821 ttccggcgcg agcgcctggg tgccgaaggc gaagaataac tcggccggcg gccgcacgca
     2881 gccaattcgg cagtgcgtgg gttgtcgtaa acggtatccg caagcggagt tggtgcggtt
     2941 cgtccgaacc ccgcaggggt gggacgccga cgccgccgga gcgcggcgcc gagcgccggg
     3001 ccggggtgcc tacctctgct cggaagcctg ccggctgacg tcggcgaaga acaaacgcta
     3061 cccgggtctt gccgcggcgg cggcggaatg tggtttaata agaagctcgt cggatggaca
     3121 acactaacgt gatgatcggc aacgtcggca accatgcgcc ctgatgcgat gaggggcgtc
     3181 aattgaacgc gtagtgggat cggtcccgta gtcgggacgg tcccaccgta aggtgaggga
     3241 acttggctac gggaaaagta cggattttcg agctcgccaa agaggtcggg ctgccctcga
     3301 aggaactgat ttcgctcttt aacgagcggc tcggcggggt cttcgaagca aaaaaccagt
     3361 tgagcgtcgt cccggatcag atcgcagatc tgattcgcag cgtcttattg aaatcgggcg
     3421 ccgccccggc caagaagccg gagcgcgccg cgcccgccgc tcccgccaag gccaaagccg
     3481 ccccggccgc tccggcgaag cgcgccgcac cccgtccggc tgcgccgcca gccccggagg
     3541 cccccgttcc
//
LOCUS       DASUCJ010000275         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_33578, whole genome shotgun sequence.
ACCESSION   DASUCJ010000275 DASUCJ010000000
VERSION     DASUCJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33578"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..194
                     /locus_tag="VFU90_03350"
     tRNA            119..194
                     /locus_tag="VFU90_03350"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:152..154,aa:Phe,seq:gaa)
     gene            complement(230..907)
                     /locus_tag="VFU90_03355"
     CDS             complement(230..907)
                     /locus_tag="VFU90_03355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5478838.1"
                     /translation="MGTTIEFTRPDGQRAPGYYAAPAQPEGAPGVVLFEEWWGLTSHI
                     RETADRLAAAGFRVLGPDLFRGRVAATGDEANHLMQGLDFGDAVSQDARGAAAYLKQQ
                     GAARVGAIGFCMGGALAMLTAMHDPGDFSSAVVFYGYPPPEAGDPATIKIPIMGHWAR
                     EDAFFKAEGVDAIERALSAAGVPHEFHRYDAKHAFYNPGGLGNYHREHAETAWDRSVD
                     FFRRTLA"
     gene            complement(912..1271)
                     /locus_tag="VFU90_03360"
     CDS             complement(912..1271)
                     /locus_tag="VFU90_03360"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEU5478839.1"
                     /translation="MSTDFRDGRTFPRRGRQAIGEALWLLRMADKARAAAAGTIHDYI
                     YPCPMDRGVMDRWGITPAEFEAALARHADDAALHAWLVERVASEKIRAANAWLLNAKA
                     ENLDRQDAEEPVLQPGG"
     gene            1337..2041
                     /locus_tag="VFU90_03365"
     CDS             1337..2041
                     /locus_tag="VFU90_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478840.1"
                     /translation="MDPPLRRRETVAHEPRWHASLAVLAALILYITLPPRLTIGPAWV
                     APLLVLALLVPLSILAPRRHAETRRMRFWGILLIAIVNFFNIVSIVLLINGFFHPARV
                     VELNSAAILLRHGVQVWLTNILVFALWFWELDGDGPEARAHAFGTRELRNADFLFPQM
                     QGSFFAGNLPAIAAPDWKPLFFDYLYLSFTNSTAFSPADAFPLTRTAKALMLVEALIS
                     LITIAIVLARSVSLIQ"
     gene            complement(2035..>2236)
                     /locus_tag="VFU90_03370"
     CDS             complement(2035..>2236)
                     /locus_tag="VFU90_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478841.1"
                     /translation="VGFCFIFRVRSQAVHLVMTLALSTVIATIFTLIALFDYPFRGTT
                     QLRPTLFVQLQRDLASTDHVVY"
BASE COUNT          349 a          753 c          725 g          409 t
ORIGIN      
        1 ctgtagctca gttggtttag agcgccgccc tgtcacggcg gaggtcgtgg gttcgagccc
       61 catcagcgtc gccagttcgc gagcgcaccg cacgacctgc ggtgcgctcg tagcaaacgc
      121 caaggtagct cagttggtag agcacgcgcc tgaaaagtgc ggtgtcgccg gttcgattcc
      181 ggcccttggc accacgcttt ccgggcctcg ccggcgacgg cggggccctc tacgcgagcg
      241 tacgcctgaa gaagtcgacg cttcgatccc aggccgtctc ggcgtgctcg cggtgatagt
      301 ttcccagccc gccggggttg tagaacgcat gtttagcgtc gtagcgatgg aactcgtgcg
      361 ggacgccggc agcgctcaac gcccgctcga tcgcgtcgac accttcggct ttgaagaacg
      421 catcctcgcg cgcccagtgc cccatgatag gaatcttgat cgtcgcgggg tcgcccgctt
      481 cgggcggcgg atagccgtaa aaaaccaccg ccgaactgaa gtcgccgggg tcgtgcatcg
      541 cggtcagcat cgccagtgcg ccgcccatgc agaagccgat cgcaccgacg cgtgcggcgc
      601 cctgctgctt cagatacgcg gcggcaccgc gcgcgtcttg tgagaccgcg tcgccgaaat
      661 cgaggccctg catcaaatgg ttcgcctcgt cgccggtggc cgccacccgg ccgcgaaaca
      721 gatccggtcc gagcacgcgg aatcccgccg ccgccaaacg gtcggcggtc tcgcgaatat
      781 ggctggtgag cccccaccac tcctcgaaca atacgacccc gggcgcgccc tcgggctgcg
      841 cgggagcggc atagtagccc ggcgcccgtt gcccgtccgg gcgggtgaac tcgatcgtcg
      901 tccccacggc gttatcctcc gggttgcaga accggttctt cggcgtcttg ccgatccaga
      961 ttttcggctt ttgcgttgag cagccacgcg tttgcggccc gaatcttctc gctcgcgacg
     1021 cgttcgacca gccacgcgtg cagtgcggcg tcgtcggcat ggcgcgcgag ggcggcttcg
     1081 aactcggcgg gcgtaatccc ccagcggtcc atcaccccgc gatccatcgg gcacgggtaa
     1141 atgtagtcgt gaatcgtgcc ggccgctgcg gcacgcgctt tatcggccat gcgaagcagc
     1201 cagagcgctt cgccgattgc ttgacggccg cgtcgtggaa aggtccggcc gtcgcgaaaa
     1261 tccgtagaca tggcctttat gacggccgcg cggcttcggc gggttgcact cgcatcgcgg
     1321 gcgaacgccc ggccacatgg atcccccgct gcgccggcga gaaaccgtcg cgcacgagcc
     1381 gcgctggcac gcctcgctgg cggttctggc cgcgctgatt ttgtacatca cgctcccgcc
     1441 gcgactcacg atcggaccgg cctgggtcgc gccgctgctg gtgctcgccc tgctggttcc
     1501 gctgtcgatc ctcgcaccgc gccggcacgc cgaaacgcgg cgcatgcggt tctggggaat
     1561 cctgctgatc gcgatcgtga attttttcaa tatcgtgtcg atcgtgctgc tcatcaacgg
     1621 gtttttccac cccgcgcggg tcgtcgaact caactccgcc gccatcctgt tgcggcatgg
     1681 ggtgcaggtt tggctcacca acatcctagt gttcgcgctg tggttctggg aactcgacgg
     1741 cgacggaccg gaggcgcgcg cgcacgcctt cgggacgcgc gagctgcgca acgccgactt
     1801 cttgtttccg cagatgcagg ggtcgttctt cgcgggcaac ttgccggcca tcgccgcgcc
     1861 cgattggaag ccgctgtttt tcgactacct ctatctgtcg ttcaccaaca gcaccgcctt
     1921 cagccccgcc gacgcgttcc cgctgacgcg cacggcgaag gcgctgatgc tggtcgaggc
     1981 gctcatctcg ctgatcacga tcgcgatcgt gctggcgcga tcggtcagcc tcattcagta
     2041 aacgacgtgg tcggtcgagg cgagatcccg ctggagctgg acgaataggg tcggacgcag
     2101 ctgggtggtt ccgcggaacg ggtagtcgaa cagcgcgatc agcgtaaaga tcgtcgcgat
     2161 gacggtcgac agcgcgagcg tcatcaccag gtgaacggct tggctgcgta cccggaagat
     2221 gaagcagaac ccgacg
//
LOCUS       DASUCJ010000276         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_369883, whole genome shotgun sequence.
ACCESSION   DASUCJ010000276 DASUCJ010000000
VERSION     DASUCJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369883"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFU90_03375"
     CDS             <1..1135
                     /locus_tag="VFU90_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5478842.1"
                     /translation="FSGLIAFSDPPLADAAETLAALRADGVEVKILTGDNELVAAHVC
                     AQAGLAAEGIVSGADIDSLSMAALGALADRTTVFARMSPVQKNRVLLALKARGHVVGF
                     LGDGINDAPALHTADVGISVASAVDVARDAAQVVLLDRDLRVLHKGIIEGRKAFGNVL
                     KYLLMGTSSNFGNMFSMAVAALVLPFLPMLPPQILLNNFLYDAAQITIPTDHVDDVLI
                     RTPRRWDMRLVRRFMVAVGPVSSIFDFVTFWVLLAVFHATEPAFHTGWFVESLVTQTL
                     VLLVIRTTGNPLRSRASRPLTWSIFGAIALGVALPFLPIAGSLGFTPLPLRFLLLLAP
                     LTLAYLVLVQVVKARVVGRWLAHDDAAGAVRSAIPVPAPASAP"
     gene            1253..>1706
                     /locus_tag="VFU90_03380"
     CDS             1253..>1706
                     /locus_tag="VFU90_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5478843.1"
                     /translation="MSSHHIAIVYGTRYGQTTRIAERMKEVLTAAGHTVWIGCADALS
                     GQFTALDYDGVIIGGSVIGGHHQRYLERFVRAHRDALNQMPSAFFSVSGSAAGAKEQD
                     QANARRVLVAFLTKTGWHPRMTATIGGAMAFTKYGLFTRMLLKRISRRA"
BASE COUNT          251 a          596 c          550 g          309 t
ORIGIN      
        1 gttttcgggt ttgatcgcct tcagcgatcc accgctcgcc gatgcggccg agacgctggc
       61 cgcgcttcgc gccgacggcg tggaggtgaa gatcctcacc ggcgacaacg agctcgtggc
      121 ggcgcacgtg tgcgcgcagg ccgggctcgc cgccgagggg atcgtatcgg gcgccgacat
      181 cgactcgctc agcatggccg cgttaggcgc actcgccgac cgcacgaccg tgttcgcgcg
      241 catgtcgccc gtgcagaaga atcgcgtcct cctcgcgctc aaagcgcgcg gccacgtggt
      301 cggattcctc ggcgacggca tcaacgacgc gcccgctctc cacacagccg acgtcggcat
      361 ttcggtcgcc agcgctgtcg atgtcgcacg agatgccgcg caggtggtgc tcctcgaccg
      421 cgaccttcgc gtgctgcaca agggcatcat cgagggccgc aaggcgttcg gcaacgtcct
      481 caaatatctc ctcatgggga cgagctcgaa cttcggcaac atgttcagca tggcggtcgc
      541 ggcgctcgtt ctgccgttcc tccccatgct gccaccccag atcctgctca acaattttct
      601 gtacgacgcc gcccagatca ccatcccgac ggatcacgtc gacgatgtgc tcatccggac
      661 gccccgccgg tgggacatgc gcctggtccg ccggttcatg gtggccgtcg gtcccgtgag
      721 ctcgatcttc gacttcgtca cgttctgggt gctgctcgcg gtctttcacg ccacagagcc
      781 ggcgtttcac acgggctggt tcgtcgagtc gctggtgacg cagacgctcg tgctcctcgt
      841 catccgcacc accggcaacc cgctgcggag ccgcgcgagc aggccgctca cgtggtcgat
      901 tttcggcgcc attgcgttag gcgttgcctt gccgtttctg cccatcgccg gctcgctcgg
      961 cttcacgccg ctgccgctgc gcttcttgct gctgctcgcg cctctgacgc tcgcgtacct
     1021 ggtgctcgtc caggtcgtca aggcgcgcgt cgtgggccgc tggctcgcgc acgacgatgc
     1081 cgcgggcgcc gtgcgctcgg cgatcccggt tcccgcgccc gccagcgcgc cgtaagccgg
     1141 cgtcaggcga cggcggacac gccgtcgtca cgtccctgac tgtccggcgc ggtcgagcga
     1201 gacaccttcc gagccaagag tcgaatcgga aaccgagcct gcggggtgca tcgtgtcttc
     1261 acatcacatc gcgattgtgt acggcacacg ctacgggcag accacgagga tcgccgagcg
     1321 gatgaaggaa gtcctgacgg ccgcgggtca caccgtgtgg atcggctgtg ccgatgcgct
     1381 gtcgggccag tttacggccc tcgactacga cggcgtgatc atcggcggat cggtgattgg
     1441 cggccaccac caacgctatc tcgagcgttt cgttcgcgca caccgggacg cgttgaacca
     1501 gatgccctcg gcgttcttct ccgtgagcgg ctcggcggcg ggagccaaag aacaggacca
     1561 ggcgaacgca cggcgcgtgc ttgtcgcgtt tctcacgaag accggctggc acccacgtat
     1621 gacggcgacg atcggcgggg ccatggcgtt cacgaagtac ggcctgttca cgcggatgct
     1681 gctcaagcgc atcagccgcc gggccg
//
LOCUS       DASUCJ010000277         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1164689, whole genome shotgun sequence.
ACCESSION   DASUCJ010000277 DASUCJ010000000
VERSION     DASUCJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164689"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFU90_03385"
     CDS             <1..547
                     /locus_tag="VFU90_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478844.1"
                     /translation="DVAVHGGTFYAAGGQAVAVCTQGGCSTELTDASIFQLETAAVDS
                     HGNVWASYYSNGGGPSLIVWPGGAMPGRTVRGYVNQNTPGGLLFDRRDRLISVQTLFP
                     HVYTYRCDASTARCRNTGTFGLQGGSLFGALNARNSNFQATDYANDSIDVYAYPGFTY
                     EYSYDRGLLSGSAVQGIVQTH"
     gene            580..>1065
                     /locus_tag="VFU90_03390"
     CDS             580..>1065
                     /locus_tag="VFU90_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478845.1"
                     /translation="MEGFGKKAFAPVRALALLTLGALVSSCAGNPGLQGAPVLSNGGA
                     NQRTTAHAISDTSRCPQSVVYVVSSQTQTVKAYDRANLGAGPCGTLSGFSSPQGLFVD
                     AKGSLWVADAATRAVFQFAPGRKAPTRELSDPSGVPIDITVDATNGTAYVTEYQNDLH
                     AG"
BASE COUNT          193 a          346 c          340 g          186 t
ORIGIN      
        1 cgacgtcgcc gtgcacggcg gaacgttcta cgccgccggc ggccaagccg ttgcggtgtg
       61 cacgcaaggc gggtgctcga cggaactcac cgacgcctcg attttccaac tcgaaacggc
      121 cgcggtggac tcgcacggca acgtctgggc gtcgtactac agcaacggcg gcggcccgtc
      181 gctgatcgtg tggccgggcg gtgcgatgcc gggccgcacc gtgcgcgggt acgtgaatca
      241 gaacacgccc ggcgggctgc tcttcgaccg ccgcgaccgg ctcatttccg tccaaacgct
      301 ctttccgcac gtctatacgt accgctgcga tgcatccacc gcgcgatgcc gcaataccgg
      361 aaccttcggg ctgcagggcg gctcgctgtt cggggcgctc aacgcgcgca actcgaactt
      421 tcaagcgacc gactatgcca acgattcgat agacgtctac gcgtatccgg gattcaccta
      481 cgagtacagc tacgatcgag ggctgttatc gggttccgcc gttcagggaa tcgtccaaac
      541 gcactgacca gctcgccggt ggcgttggag gaaccataaa tggaagggtt tggaaagaaa
      601 gcgttcgcgc ccgtccgtgc gctcgcgctg ctgacgctcg gagcgctcgt ttcctcctgt
      661 gctggtaacc ctgggttgca gggcgcgccg gtattatcga acggcggtgc gaaccaacgg
      721 acgacggcgc acgcgatcag cgatacgtcg cgctgcccgc aatcggtcgt ctacgtcgta
      781 tcgagccaaa cgcaaacggt caaagcgtac gaccgggcaa acctcggggc gggaccgtgc
      841 ggcacgctga gcggtttttc ttcgccgcag ggattgtttg tggacgcgaa gggcagcctc
      901 tgggttgccg atgccgcgac gcgagcggtc tttcagttcg ctccgggaag gaaggcgccc
      961 acgcgcgagc tctccgatcc cagcggcgtt ccgatcgaca tcactgtcga cgccaccaac
     1021 ggcacggcct acgtgaccga atatcaaaac gacctgcatg ccggc
//
LOCUS       DASUCJ010000278         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1027073, whole genome shotgun sequence.
ACCESSION   DASUCJ010000278 DASUCJ010000000
VERSION     DASUCJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027073"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1469
                     /locus_tag="VFU90_03395"
     CDS             <1..>1469
                     /locus_tag="VFU90_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEU5478846.1"
                     /translation="DTVLFAVLGNTYGGNATSIFALPDLSDRANVAAGTGSGLTTQTL
                     GAPTGSASVTLLTTQIPSHTHSVPSGGSTGATGGGLPFDNHEPSLPLTRLIAFDVGAS
                     GGAVFAGQVATFAGNYAPDGWLVADGSVLDPTLYPALFAAIGTTYGGDGVTTFALPDL
                     RGRVSVGADATHPLGTAFGEEATTVTTMTLPAHNHTIPSGGSTGVTGFGLPLENQQPS
                     LALNYLVATQGIFVGQSSGSFDADSPILGQIVEYAGNTPPTGWAIADGSQLQISTNAA
                     LFNVIGTLYGGDGLRTFDLPDLRGRTIAGASITNAAGTPTGSASVTLTIANLPAHTHT
                     LPLPTITSASYDASSGALVVTATDLLANGSGLDIDATKFTLTGEGGATYTLTDTPSVE
                     IASATSFTLTLSATDQAAVNQILNRNGTASTGGTTYNLAAAEDWNTGANPADVIADLT
                     GNGITVSNVAAPSIAGATYDALAGTLAVTGTGFLQAAGA"
BASE COUNT          245 a          551 c          469 g          204 t
ORIGIN      
        1 ggacacggtg ctgttcgccg tgttgggaaa cacgtacgga ggcaacgcca cgtcgatatt
       61 cgccctgccg gacctctccg atcgcgcgaa cgtcgcggcc ggaaccggca gcggactcac
      121 cacgcagacg ctgggcgcgc cgaccggaag cgccagcgtg acgctactca cgacgcagat
      181 tccctcgcac acgcactcgg tccccagcgg cggatccacg ggtgccacgg gcggcgggct
      241 gcctttcgac aaccacgagc cgtcgcttcc gctcacgcgc ctcatcgcgt tcgacgtcgg
      301 cgcgagcggc ggggcggtct tcgccgggca agtcgcgacg ttcgcgggca actacgcccc
      361 ggacgggtgg ttggtcgcgg atggaagcgt tctggatccc accctgtacc ccgcgctctt
      421 cgccgccatc ggcaccacgt acggaggcga tggggtcacc accttcgcgc tgccggactt
      481 gcgcggccgc gtttccgtcg gtgccgacgc gacccatccc ctgggaacgg cgttcggcga
      541 ggaagcgacg acggtgacga cgatgacgct tcccgcgcac aaccacacga tcccgtccgg
      601 cggatccacg ggcgtgaccg gcttcggcct cccgctggaa aaccagcagc cttcgctcgc
      661 gctcaactac ctggtcgcga cgcagggaat attcgtgggc caatcgagcg gctcgttcga
      721 tgcggactcg ccgatcctcg gccagatcgt cgagtacgca ggcaatacgc cgccgaccgg
      781 atgggcgatc gcggacggct cgcagctgca gatctccacg aacgcggcgc tgttcaacgt
      841 gatcggcacc ctgtacggcg gcgacggcct gcgcaccttc gaccttcctg acctgcgggg
      901 acgcacgatc gccggggcga gcatcaccaa cgccgcgggc acgccgaccg gaagcgcgtc
      961 ggtcacgctc acgatcgcga acctgcccgc ccatacccac acgctgccgc tgccgacgat
     1021 cacttcggcg tcctacgatg cctccagcgg ggcgctcgtc gtcaccgcga ccgacctgct
     1081 cgcgaacggc agcggcctcg acatcgatgc caccaaattc acgctcaccg gcgaaggcgg
     1141 cgcgacttac acgctcaccg atacgccgag cgtggagatc gcctccgcca cgagcttcac
     1201 gctcacgctg tcggccaccg accaggcggc cgtgaaccag atcctgaatc gcaacggcac
     1261 cgcgtctacc ggcgggacga cgtacaacct cgcggccgcg gaggactgga acacgggcgc
     1321 caatcccgcg gacgtcatcg cggacctcac cggcaacggc atcaccgtct cgaacgtagc
     1381 ggcgccttcg atcgccggtg cgacctacga cgccctcgcc ggaacgctcg cggtgacggg
     1441 caccggcttc ctgcaggcgg cgggcgccg
//
LOCUS       DASUCJ010000279         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1179828, whole genome shotgun sequence.
ACCESSION   DASUCJ010000279 DASUCJ010000000
VERSION     DASUCJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179828"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1395
                     /locus_tag="VFU90_03400"
     CDS             16..>1395
                     /locus_tag="VFU90_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5478847.1"
                     /translation="MAGFNQLRSIRVPVLGLSVIPLALLLIVVGAIAWLDVETVASAA
                     WAQRSADVLESASALQNDLASAQSAVQEYQLTGSPRGAVAFEHAAAAVPRDAGRLRLS
                     ELENPSQAALARQLEAGAVADVHDLRVTMNRLRQGVRPAIDRQGEALQDKLRATLAKF
                     ESAENQLEQKRRATSYRLWLELFLVLLGSTIVGAALTLVLNLTFGRRIVRRLRNVTNQ
                     TLAFANDGEILDPLRGRDELANLSRSVRDLATQIKERDAALVRYQLLAENVRDIILFA
                     RHADGKILDANYSAATAHGYTRAELLGLTVAVLHHPSAHESLSSMLRRTEVRDLYIET
                     IHRRKDGSTFPAEALAQSVTIDGEHVVVCIVRDISERHASEQAVRMALNQAMEASRLK
                     SDFVATMSHEIRTPMNGVVGMTELLLETHLTPQQREYAATARDSAHSLLGIINNILDF
                     SKIEAGKVDL"
BASE COUNT          239 a          461 c          460 g          235 t
ORIGIN      
        1 ttagggaggg tgctggtggc gggctttaac cagctgcgtt cgattcgcgt cccggtgttg
       61 gggctgagcg ttattccgct cgccctgctt ctgatcgtgg tcggcgcgat cgcgtggctg
      121 gacgtcgaaa cggtcgcctc cgccgcctgg gcgcagcgtt cggccgacgt cctcgagagt
      181 gcgagcgcgc tgcaaaacga ccttgcgtcc gcgcaaagcg ccgtgcaaga gtaccagctg
      241 acgggctcgc cgcgcggcgc ggtcgcgttc gagcacgccg cggccgcggt tccgcgcgac
      301 gccggccggc tgaggctgtc cgagcttgag aacccctcgc aggctgccct cgcgcggcag
      361 ctcgaggcgg gtgccgtcgc cgacgtccac gatttgcggg tcaccatgaa ccggcttcgc
      421 caaggcgtcc ggccggcgat cgaccggcag ggcgaggcgt tgcaagataa actgcgcgcc
      481 acgcttgcga agtttgagtc ggcggagaat caactggagc agaagcggag agccacgagc
      541 taccggttgt ggctcgagct gttcctcgtg ctgctgggaa gcacgatcgt cggtgccgcc
      601 ctgacgctcg tactgaacct taccttcggg cgccggatcg tccgccggct gcggaacgtg
      661 accaatcaaa cgctggcgtt cgccaacgac ggcgaaattc tcgatccgct acgaggacgg
      721 gacgagctcg cgaatctctc gcgctcggtt cgcgacctgg cgacccagat caaggagcgc
      781 gacgccgcgc tggtccgcta ccagctgctc gccgagaacg tgcgcgacat catcctgttt
      841 gccaggcacg ccgacggaaa aatcctcgac gccaactaca gcgcggcgac ggcccacggc
      901 tacacccgcg ccgagctgct cgggcttacg gttgccgttc tgcaccatcc gtcggcgcac
      961 gagagcttgt cgtcgatgct gcggcgcacg gaagtgcggg acctgtacat cgagaccatt
     1021 caccgccgca aggacggctc gaccttcccg gcggaagccc tcgcgcagag cgttaccatc
     1081 gacggcgagc acgtcgtggt ctgcatcgtg cgcgacattt ccgagcggca cgcttcggaa
     1141 caggccgtac gcatggcgct gaatcaggcg atggaggcgt cgcgcctgaa gagcgatttc
     1201 gtcgccacca tgagccacga gattcgcacg ccgatgaacg gcgtcgtcgg gatgaccgag
     1261 ctgctgctcg agacgcattt gacgccgcag caacgcgagt atgcggcgac cgcgcgcgat
     1321 tccgcccact cgctgctcgg catcatcaac aacattctcg atttttcgaa gatcgaagcc
     1381 ggcaaagtcg atctc
//
LOCUS       DASUCJ010000280         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_843783, whole genome shotgun sequence.
ACCESSION   DASUCJ010000280 DASUCJ010000000
VERSION     DASUCJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843783"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1214)
                     /locus_tag="VFU90_03405"
     CDS             complement(411..1214)
                     /locus_tag="VFU90_03405"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEU5478848.1"
                     /translation="MVFDRDGNLYVTDTDGRRNSRVVKIAPDGRPVAQWHIFAAPAGK
                     RNGPEGIAMTASGTILVTDAGIERILEVSPQGRVLRNFGGTEGVFKDLGHVAVDPAGN
                     VYVAQAEPNVIEKYSAAGALLDAWHRSKGPGVGEWGGPETIATQPDGHLVVEDWGNRR
                     IEIFDSSGTMLLAFGHLGNGPGEFRNTAGLGVDGDGDIYVTDVALHRVQKFDARGRLL
                     ATIANAPGAALFAKGPSAIAIDRQGSLYSPDGQTVVKYSQDGKLLARWQ"
BASE COUNT          360 a          631 c          585 g          341 t
ORIGIN      
        1 ttgaacgcct cggtctgcac aaaccaatcg tggagctcga gcccgcgcac gccaagcgga
       61 ttatcgagat cctgccgcgg accggcgccg aatccgtcga gtgagaccga aaacgcagcg
      121 acccgagtct tgctcattcg ctgggcacac tccgcgccga cccgactcag cccctgctat
      181 aaatcgggca agccctttca aatagaagca gcccgcgttc ggcgccgggc aaagtgcttg
      241 acagttgaag cagcgcgaga acgacgaacg ccagcacgga agcgatcagg accggcgtaa
      301 aatcgattcc ggacgacgat cctttccggg aattcatgag gcgcatactt cttatacgcc
      361 cggccgccca ttccggatgt ccgcctgccg ggttcgaccg tcgaaactcg ctactgccag
      421 cgtgcgagca gtttgccgtc ttgggagtac ttcaccaccg tctgcccgtc cggcgaatag
      481 aggcttccct ggcgatcgat cgcaatcgcg ctggggcctt tcgcaaagag cgccgctccg
      541 ggcgcattcg cgatcgtcgc gagcaggcgg ccgcgcgcgt cgaacttttg tacgcgatgc
      601 agcgcgacgt cggtgacgta gatatcgcca tccccgtcga cgccgaggcc ggccgtattg
      661 cggaactcgc ccggcccgtt tccaaggtgc ccgaacgcga gcagcatcgt tccggatgaa
      721 tcgaaaattt cgatgcgccg gttgccccaa tcttcgacga ctagatggcc gtcgggctgc
      781 gtggcgatcg tctccgggcc gccccactcg ccgacgcccg gacccttcga ccgatgccac
      841 gcgtccaaaa gcgcgcccgc tgcggagtac ttttcaatca cgtttggctc ggcttgtgcg
      901 acgtaaacgt tgccggcggg atcgactgcg acgtgtccga gatccttgaa gacgccctcg
      961 gttccgccga agtttcgcag cacgcgccct tgcggcgaaa cttcgaggat ccgttcgatg
     1021 ccggcgtccg ttacgaggat cgtccccgac gcggtcatcg cgatcccttc gggaccgttg
     1081 cgtttgccgg ccggtgccgc aaaaatgtgc cactgcgcca ccggcctgcc gtccggcgcg
     1141 attttaacga cgcgcgagtt ccgccggccg tcggtatcgg tgacgtacag attgccgtca
     1201 cgatcgaaca ccatgttatc gggcccgtaa aaacagatct tgcagggcgc tcgttctccc
     1261 ggcgtcgacg tcgcaccgag cccgaacgcg aggatcgcgg tgacggcagc aatcgcgcag
     1321 cgggacagcc gtctcccgtt ctgtcggacc ggaccttcca gtgcgcgcat gtcacaaagt
     1381 gaaccctgag gggcttgcgg ccctgctgct agcgcgccgc ctgcaccgca acgcgatttg
     1441 gccgaagttg cgcgacgacg gaaggatatc gggtatcgtc ctgccggagt cacctgctcc
     1501 ccgtgtaggc cagggctcgg cgcggccggc ctgaaactcg tgcatccacg ctgagccccg
     1561 aagcggcgtt gaccgcggca ttacgcacgc acgttcaggc ttcaaaaaag gcccgcgaac
     1621 gactacgata gaggatacgg ccctcttggg gatttgcccg tcgttgaagg agatcgaatc
     1681 gtgtccacaa ccgaatgtcc cgttcataag ccccgcgcca ggcggacgag ccgcgattgg
     1741 tggccggaac aacttcgtct gtcgatactg cggcagaact cgcccgaatc ggacccgatg
     1801 ggtgaggact tcgactaccc caaggagttc gcgaagctcg atctcgcggc ggtaaagcgc
     1861 gacttagcgg cgctcatgac gcaatcgcag gagtggtggc ccgcagattt cggaaac
//
LOCUS       DASUCJ010000281         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1057601, whole genome shotgun sequence.
ACCESSION   DASUCJ010000281 DASUCJ010000000
VERSION     DASUCJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057601"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..>1909
                     /locus_tag="VFU90_03410"
     CDS             101..>1909
                     /locus_tag="VFU90_03410"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5478849.1"
                     /translation="MQKHDKLPDPESDRPVIDVTRRDFLVRAGLYGGALTLFSRAPSM
                     VRDVVRHPSHALADAPTALAAPAADLSPLFSGLQWRLLGPFRGGRTDAVTGVPGRPNE
                     FYSGSVNGGLWKTIDAGRVWEPVFDSNPVASIGAVAVAPSQPDTVYVGSGECTLRDST
                     GFGNGVYKSTDAGKTWTHLGLDATQHIGKVAVHPTDPNTVFVAAIGNFYGPTTERGIY
                     RSTDGGRSWKKVLFKNENVGAIEVVIDPTNPKVIYAGLWNTRRPPWFVYAPTNGPGGG
                     IYKSTDGGDSWTELTNGLPKEGIGKSGIAVCPSNPQRLYAVIDDLVQDPSIPVDTSQA
                     AVAARRFGVGVPGLGGFFRSDDAGASWTRLSNDSALWGRGWYFEHVAVDPKNADVVYV
                     SNVSVSRSMDGGHTWVPLRGSPGGDDYHQPWVSPDDPNTLIVASDQGTVITRNAKSED
                     PRDVSWSSWLNQPTAQIYHASVDFRFPYWVTGAQQDSGAVAVRSRGKFGEISMRDWEP
                     IGAGGESGYTAGDPLHPGIVFGGTGQRFDLALNVPIAGTTAPAVPKGETARADWTQPL
                     VVSRADPRSLYYGNQYVFLSTDCAQTWTRVSPDLTRA"
BASE COUNT          325 a          643 c          611 g          330 t
ORIGIN      
        1 cgcttgacac gcgctgtacc gcgttctatt tgacagcgcg acgcatgcga ccgacatgac
       61 gcgatgctcg cacgcatcct cgcacctcga gagaccgact gtgcagaaac acgacaagct
      121 tccggacccc gaatctgatc gccccgtcat cgatgtcacc cggcgcgact tcctcgtgcg
      181 cgccggactg tacggtggcg cgctcacctt gttttcccgc gccccctcga tggttcgcga
      241 tgtcgtgcga cacccatcgc acgcgctcgc cgatgcgccg accgcgctcg ccgcgccggc
      301 cgccgatctc tcaccgctct tttcgggatt gcagtggcga ctgctcggcc ccttccgcgg
      361 cggacgcacc gatgccgtga cgggcgtgcc gggccggcct aacgaattct attcgggttc
      421 cgtcaacggc ggtctctgga aaaccatcga cgccggacgc gtgtgggaac cggtgttcga
      481 ttccaatccc gtcgcgtcga tcggcgcggt tgccgtggcg ccgtcccagc cagacaccgt
      541 gtatgtcggc agcggcgagt gcacgctgcg cgactcgacg ggcttcggca acggcgtgta
      601 caagtcgacc gatgccggca agacctggac gcatctcggc ctcgacgcga cgcagcacat
      661 cggcaaggtc gccgtgcatc cgaccgatcc gaacaccgtg ttcgttgccg caatcggaaa
      721 tttctacgga ccgaccaccg aacgcggcat ctatcggtcg accgatggcg gacgcagttg
      781 gaagaaagtg ttgttcaaga acgagaacgt cggcgcgatc gaggtcgtga tcgatccgac
      841 gaacccgaaa gtcatttacg ccggactctg gaacacgcgc cgcccaccct ggttcgtgta
      901 cgcccccacg aatggtcccg gcggcggcat ttacaaatcg accgacggcg gcgactcgtg
      961 gaccgaattg acgaacgggt tgccgaaaga aggcatcggc aagagcggca tcgccgtgtg
     1021 cccgagcaat ccgcagcggc tgtatgcggt gatcgacgac ctcgtgcagg atccgagcat
     1081 tcccgtcgac acgtcgcagg ccgccgtcgc cgcgcgccgg ttcggcgtcg gcgttcccgg
     1141 gttaggcggc ttcttccgct ccgacgatgc gggcgcgtcg tggacgcgcc tgtccaacga
     1201 ttccgccctg tggggacgcg gctggtactt cgagcacgtt gccgtcgatc ccaagaacgc
     1261 ggatgtcgtg tacgtgagca acgtgtcggt ctcgcgttcg atggatggcg gccacacgtg
     1321 ggtgccgctg cgcggttcac cgggcggcga cgactaccat cagccatggg tgtcgcccga
     1381 tgatccgaac acgttgatcg tcgccagcga tcagggcacc gtcatcacgc gcaatgcgaa
     1441 gagtgaagat ccccgcgacg tgtcgtggag ctcgtggctc aaccaaccga ccgcgcagat
     1501 ctatcacgcc tccgtcgact tccgcttccc gtactgggtg accggcgccc aacaagacag
     1561 cggcgctgtt gcggtgcgct cgcgtggaaa gttcggcgag atttcgatgc gcgattggga
     1621 gccgatcggc gcgggcggcg agagcggata tacggcgggc gatccgctcc atcccggcat
     1681 cgtgttcggc ggcacaggcc agcgcttcga tctcgcactc aacgtgccga tcgccggcac
     1741 cacggcgccg gcagtgccga aaggcgagac cgcgcgcgcg gactggacgc agccactcgt
     1801 cgtatcgcgc gccgatccgc ggtcgctcta ctacggcaat cagtacgtgt tcctgtctac
     1861 cgactgcgcg cagacctgga cgcgtgtgag tccggatctg acgcgtgcg
//
LOCUS       DASUCJ010000282         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_18413, whole genome shotgun sequence.
ACCESSION   DASUCJ010000282 DASUCJ010000000
VERSION     DASUCJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18413"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFU90_03415"
     CDS             complement(<1..184)
                     /locus_tag="VFU90_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478850.1"
                     /translation="MAFTERCRVYTPAILLARTVLACAVAAAPAACSKSAAPVLRHPG
                     TFVVATPQEPVSLNPLY"
     gene            292..855
                     /locus_tag="VFU90_03420"
     CDS             292..855
                     /locus_tag="VFU90_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478851.1"
                     /translation="MRTILAFTIAATLTAAAARAADPYAAVDAMRTAFAQVRSATAVE
                     HFSSGDVATVNYNAPNRYRITTEKSEIVVTGDTEYAKRKGGEWVLSPHGAMHQEILKS
                     VWQLAGRPGTDLHKLYKIVPLGSKVVDGTAVRGYHLADTTGGYDETLWIGANDLPLEA
                     QISMTGQTIDIHFADYNRSMLVATPIR"
     gene            900..1325
                     /locus_tag="VFU90_03425"
     CDS             900..1325
                     /locus_tag="VFU90_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5478852.1"
                     /translation="MIAALVLWGLSVANLAVAQTYPTPMPYATPMPYATPVPYSSPGP
                     GGSSCPETDVQIVKAAQPEYPAAAMKFIINPVSVQVIVMVNADGSVQTASIARSSGYS
                     QPDASALKAAEESTYKPKTVNCKPVRGLYVFQAVFKPQQ"
     gene            complement(1332..1778)
                     /locus_tag="VFU90_03430"
     CDS             complement(1332..1778)
                     /locus_tag="VFU90_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5478853.1"
                     /translation="MRSARDVERLHALLTEYEERLPADPRHGSVPDLAAVERRYGDPN
                     AAFLAIGEGVAAGCVAVTRFDASRARLRHLFVTPAFRGHGLARRLTEAAIAFAAESGY
                     GVLALDTEKTRLAAAYRLYVSLGFEECAPYGPVDYETPTFMQLRLR"
BASE COUNT          357 a          604 c          602 g          293 t
ORIGIN      
        1 aatacagcgg gttgagcgag acgggctcct ggggcgtggc gacgacgaac gtgccgggat
       61 gccgaagcac gggtgcggca ctcttcgaac acgccgccgg cgcggcggcc acggcgcacg
      121 cgaggaccgt acgggcaagc aagattgccg gcgtgtaaac gcggcaacgt tcggtgaaag
      181 ccatgctcgt tcggtcagta tagcatgccg ccgactgcga gcaatcgccc ggtcggccta
      241 tttttacgaa cgtccgagcc cggcgcgccg tcaaatgggt aaaagcgggt catgcgcacg
      301 atattagcct tcacgatcgc agccacgctg acggcagcgg cggcgcgtgc tgccgatcct
      361 tacgccgcgg tcgacgccat gcgtacggcg ttcgcgcagg tgcgatccgc aaccgccgtc
      421 gagcatttca gcagcggcga cgtcgctacg gtgaattaca acgcccccaa ccggtatcgc
      481 attaccacgg aaaaatcgga gatcgtcgtg accggcgaca ccgagtacgc caagcgtaaa
      541 ggcggtgagt gggtgctctc gccgcacggt gcgatgcatc aggaaattct gaaatcggtc
      601 tggcagctgg ccgggcgccc cggcacggat ctgcacaaac tctacaagat cgttccactc
      661 ggatcgaagg tcgtcgacgg aacggcggtt cgcggctatc acttggccga tacgaccggc
      721 ggttacgacg aaacgctctg gattggagca aacgatttgc cgctcgaagc gcagatctcg
      781 atgaccggtc agacgatcga catccacttc gccgactaca accgctcgat gttggtcgcg
      841 acgcccatcc ggtagccccg aaccaggcgg gcggcaatcg acctgcgaac cctctccgca
      901 tgattgccgc cctggtgctc tgggggctgt ccgtagcgaa tctcgcggtg gcgcagacct
      961 acccgacgcc gatgccgtat gcgacgccga tgccgtacgc gacgccggta ccgtactcga
     1021 gccccgggcc gggcggcagc tcgtgccccg agacggacgt gcagatcgtc aaggcggcgc
     1081 agcccgagta tccggcggcc gccatgaagt tcatcattaa cccggtttcg gtgcaagtca
     1141 tcgtgatggt caacgccgac ggatcggtgc agaccgcgtc gatcgcacgt tcgagcgggt
     1201 attcgcagcc cgacgcttcg gcgctcaaag cggccgaaga gagcacctac aaaccgaaga
     1261 cggtcaactg caagccggtg cgcggactgt acgtctttca ggcggttttc aaaccgcagc
     1321 agtaaggcgc gctagcgcaa gcgcagctgc atgaaggtcg gcgtttcgta atcgaccgga
     1381 ccgtacgggg cacattcctc aaaacccagc gacacatata atcggtacgc cgcggcgaga
     1441 cgcgtctttt cggtgtcgag tgccaagacg ccgtagccgc tttcggcggc gaaggcaatc
     1501 gcggcctcgg tcagccggcg ggcgagcccg tgaccgcgga atgccggcgt gacgaacagg
     1561 tggcgcaggc gcgctcggga cgcgtcgaac cgcgtgaccg cgacgcagcc cgccgcgacg
     1621 ccctcgccga tcgcgagaaa cgccgcattc ggatcgccgt atcgccgctc gacggctgct
     1681 agatcgggaa ccgaaccgtg cctgggatcg gccggcaagc gttcttcgta ttccgtcagc
     1741 agcgcgtgca gccgttcgac atcgcgcgcg ctgcgcactc gccggatctc tggggtagcc
     1801 accgccgtcc ggttggcgaa actcccgagc gaacctcgcg ccgccgcgag ctacat
//
LOCUS       DASUCJ010000283         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_568717, whole genome shotgun sequence.
ACCESSION   DASUCJ010000283 DASUCJ010000000
VERSION     DASUCJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568717"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFU90_03435"
     CDS             complement(<1..855)
                     /locus_tag="VFU90_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEU5478854.1"
                     /translation="MLTGLSPVRSGSFQALPGGRTQSLDAGSLPVGYYAVSVGDGNMQ
                     LCWMFSVSSFGLATVGAGTSAVWAVDLSTFDHLKQPVSMWVYGQHGERRLAVDADAIA
                     FDTAENGDGGGMVMASAADGSYQIVGEVGSGMAEGGTLVSTDRPIYRPGQTVNIRAIL
                     RDGGVDAYTIPHGQVRLKVSSNDGNRALAERMLRISPFGTVSTSVRLPHDVPIGVYEV
                     SVGPKRPNGYFSVVAYKKPEFTLHVATARPWTIAGDPTTVRVTAKYLFGRPATGLRLH
                     YAMTLLGTY"
BASE COUNT          233 a          391 c          364 g          203 t
ORIGIN      
        1 gtacgttccc aagagcgtca tcgcgtagtg cagccgtagg ccggtggcgg ggcgcccaaa
       61 cagatatttc gccgttaccc ggaccgttgt aggatcgccg gcgatcgtcc acggtcgagc
      121 cgtcgccacg tgcaacgtga actcgggttt cttgtaggcg acgacgctaa agtagccgtt
      181 tgggcgtttc ggcccgacgg aaacctcata cacgccgatc ggcacgtcgt gcggcaaacg
      241 aaccgacgtg ctcaccgttc cgaacggcga gatgcgcagc atccgctccg cgagcgcgcg
      301 gttgccatcg ttcgaggaaa ccttcagccg aacctgaccg tgcggaatcg tgtatgcgtc
      361 gacgccgccg tcgcgtagaa tcgcgcgaat gttgacggtt tgcccgggcc ggtaaatcgg
      421 ccggtccgtt gagacgagcg ttccgccctc ggccatgccc gatccgacct cgcccacgat
      481 ctgatagctg ccgtcggccg cggacgccat caccatcccg ccgccatcgc cgttttccgc
      541 cgtatcgaag gcgatggcgt cggcgtcgac cgcaagccgc cgctccccgt gctgaccgta
      601 cacccacatc gagactggct gtttcaagtg atcgaaggtg ctcaagtcca ccgcccaaac
      661 ggcggaggtt cctgccccga ccgtcgcgag cccgaacgaa ctgaccgaaa acatccagca
      721 cagctgcatg ttgccgtcac cgaccgaaac ggcgtaatac cccaccggga gcgatccggc
      781 gtcgagtgat tgggtgcgtc cgccaggcaa cgcttgaaac gagccgctgc ggaccggact
      841 gaggccggtc agcatggcgg gatcccgcgt gtttcggaag tacgcgatcg cacgcgacag
      901 cggcagcgca taaaccttgg ctagcgaagc cggcggatcc gcgactgtga gtacgacgtg
      961 ttggcccggc aaatacgtgt cggggctcag attctcgggg tatcttgctc cgacgatcct
     1021 gcctgaaacc ggtgcgacga atagcagaag caggagtgcc gaaaagccaa cgctccgcgt
     1081 caccattacg ctagtaacaa cgcgccaccg tctccacttt gtgacgaaca atgcagcatc
     1141 cgtgtcatcc agagcggagc gccgcatccg tgtcatcctg agcggagcgt g
//
LOCUS       DASUCJ010000284         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_232373, whole genome shotgun sequence.
ACCESSION   DASUCJ010000284 DASUCJ010000000
VERSION     DASUCJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232373"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFU90_03440"
     CDS             <1..952
                     /locus_tag="VFU90_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5478855.1"
                     /translation="FSNGSSNGTFLVFDNAGVKIVPDDFEALFKDKSRTGYATYRTAT
                     GGYFKAMGIPVVAGRVFDASDRAGGPEVAVINAALAHKQWPNGDAIGKAIEFGNIDGD
                     LTPMTIVGIVGDTREEDLAAEPASSVYVDFDQRPGNSNEMFVIMAANNEGSAIAAARR
                     SFPQVRADVPMRFLTVEDIIGRSVDSQRFMLLLVGVFGAVALLLATLGVYSVISYLVA
                     QRGREISIRVALGASASEIVRLVLQQGIALSLVGAVIGGVAALAATRVLKKLLYQVST
                     TDPIAFASVLVILCGVALVASYIPARRAAAFEPMDVLRGG"
     gene            complement(1048..>1727)
                     /locus_tag="VFU90_03445"
     CDS             complement(1048..>1727)
                     /locus_tag="VFU90_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5478856.1"
                     /translation="SAALAIVALIVARVIFPVPSRLEQESDRRAAPTGFTSRFWLFVL
                     AAGCFGAGLMSFEFISFHLSSHHVVSMAWIPLFLAISTGVGVFVNLGLGKLYDRAGLR
                     VVLVAIFVSSLFSPLVFLGGFGAVLAGLILWGVGYATQDTLLKAVLAGMLPEGKRNLA
                     FGLFYTGYGVGWLIGSTVTGLLYRSSIPAVIVFAMAVQLASIPIFVIAERKHAAIPTR
                     PDLHLRS"
BASE COUNT          327 a          553 c          566 g          281 t
ORIGIN      
        1 gttctcgaac ggcagcagca acggcacgtt tctcgtgttc gacaatgcgg gcgtcaaaat
       61 cgttccggac gatttcgagg cgctgttcaa ggacaagtcg cggaccggct atgcgacgta
      121 tcgcaccgcg accggcggct acttcaaggc gatggggatt cctgtcgtcg cgggccgtgt
      181 gttcgacgcg agcgaccggg cgggcgggcc cgaggtcgcg gtgatcaacg ccgcgctcgc
      241 ccacaagcag tggcctaacg gtgacgccat cggcaaggcg atcgagttcg gcaacatcga
      301 cggcgacctg acgccgatga cgattgtcgg catcgtcgga gacacgcgcg aagaggacct
      361 ggcggccgaa ccggcatcct ccgtctacgt cgatttcgac cagcgtcccg gcaacagcaa
      421 cgagatgttc gtgatcatgg ccgcgaacaa cgaagggtcg gcgattgccg ccgcgcgccg
      481 ctcgttcccc caggtgcgcg ccgacgtgcc catgcgattt ctgacggtgg aagacatcat
      541 cggccggtcg gtggacagcc agcgattcat gctgctgctc gtcggcgtgt tcggcgcggt
      601 cgcgctcctc ctggcgaccc tcggcgtcta cagcgtgatc tcgtatctcg tggcgcagcg
      661 cgggcgcgag atcagcatcc gtgttgcgtt aggcgcgtcg gcgtcggaga tcgtacgcct
      721 cgtgctccag cagggcatcg cgttgtctct ggtcggcgcc gtcatcggtg gcgtcgcggc
      781 gctcgctgcc acgcgcgtgc tcaagaagct gctctaccag gtgagcacca ccgatccgat
      841 cgcgttcgcg tccgtgctgg tgatcctgtg cggcgtggcg ctcgtggcca gctacatccc
      901 ggcgcggcgc gccgccgcat tcgaacccat ggacgtgttg cgtggtggct gagccgcgcg
      961 ctgtcactgg gacctacgct catgaggaaa cgggcaaagc acagactacc cgtgctttgc
     1021 ccgtttgtcc gtgctttgcc cgtttcctca tgagcgtaga tgcaagtcag gccgggtcgg
     1081 gattgcggca tgctttcgtt cagcaatgac aaaaatggga atcgacgcga gctgcacggc
     1141 catggcgaac acgatcactg ccggaatcga gctgcgatag agcagcccgg tgacggtgct
     1201 gccgatcaac caccccacgc catagccggt atagaacagg ccgaacgcga ggttgcgttt
     1261 gccttcgggc agcatccctg cgagcacggc cttgagcagc gtgtcctgcg tggcatagcc
     1321 gacgccccac aaaatcagtc cggccaacac ggcgccgaag ccgcccagaa agacgagcgg
     1381 cgagaagagc gagctgacga agatggccac gagcactaca cgcaggccgg cgcgatcgta
     1441 gagcttgcct aacccaagat tgacgaacac gcccacaccc gtcgagatcg cgaggaacag
     1501 cggaatccac gccatcgaca ccacgtggtg gctggacaga tggaacgaga tgaactcgaa
     1561 gctcatgagg cccgcgccga agcatccggc cgcgagcacg aacagccaga accgcgaggt
     1621 gaatccggtg ggtgcggcgc ggcggtcgct ctcctgttcc aaccgcgacg gcaccgggaa
     1681 aatcacgcgc gcgacgatca gcgcaacgat cgcgagcgcc gccgaca
//
LOCUS       DASUCJ010000285         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1332720, whole genome shotgun sequence.
ACCESSION   DASUCJ010000285 DASUCJ010000000
VERSION     DASUCJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332720"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..>1070
                     /locus_tag="VFU90_03450"
     CDS             185..>1070
                     /locus_tag="VFU90_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5478857.1"
                     /translation="MKRPLSIALGLLAVIAVIGLAFLVRMQFATHPLAWTTPADGGSP
                     ASSDADMHKIKHVVIIMQENRSFDSYFGTYPGADGIPMKDGVPTVCVPDPKTATCVKP
                     YHERRDVNHGAPHGAAAASKDVAGGKMSGFVAVARSGKPCEGMVDPECSGGDPHEVMG
                     YHDARELPNYWRYAHDFVLQDKMFEPNSSWSLPAHLFLLSEWSANCLVPDDPMSCHDA
                     LDRPGLPIDFAHVHDKKLRKLAGKPDYAWTDLTYLLHKHGVSWGYYIKEGRAPDCDDP
                     NDAQCAPSALKPRTPGIWN"
BASE COUNT          201 a          364 c          341 g          164 t
ORIGIN      
        1 ggccggacgt gcgagagaac gcgccgcagc tcggcgatct gcgcgccgat ttcgacttca
       61 cgcagccgcc gagaccgccg atactgctgc cgggcggcat cgatccgctg ccgcactgac
      121 ttgagtcgca cgcgatggat gccgattcat cgctgattca ccgcatcaaa gtatcctggc
      181 ggctgtgaaa cggcctctat cgatcgccct cggtcttctc gccgtgatcg ccgtcatcgg
      241 gctcgccttt ctcgtccgca tgcagttcgc gacgcatccg ctcgcatgga cgacgccggc
      301 ggacggcggg tcgccggcaa gcagcgacgc ggatatgcac aagatcaaac acgtcgtcat
      361 catcatgcaa gagaaccgct ccttcgacag ctacttcggc acgtatcccg gcgccgacgg
      421 catcccgatg aaagacggcg tgccgaccgt ctgcgtgccg gatccgaaga ccgcgacgtg
      481 cgtgaagccg tatcacgaga ggcgcgacgt caaccacggc gcgccgcacg gcgccgccgc
      541 cgcgagcaaa gacgtcgccg gcggcaagat gagcggcttc gtcgccgtcg cgcggtcggg
      601 caagccgtgt gaagggatgg tcgatcccga atgcagcggc ggcgacccgc acgaggtcat
      661 gggataccac gacgcgcgcg agctgccgaa ctactggcgc tacgcgcacg acttcgtgct
      721 ccaggacaag atgttcgagc cgaactcatc gtggagcttg cccgcgcatt tgttcctgct
      781 ctccgagtgg tcggcgaact gcctcgtgcc ggacgatccg atgagctgcc acgacgcgct
      841 cgaccggccc ggcttgccga tcgatttcgc gcacgtgcac gacaagaagc tgcgcaagct
      901 cgccggcaag ccggactacg cgtggacgga cctgacgtac ttgctccaca agcacggcgt
      961 cagctgggga tactacatca aggaagggcg agcgcccgac tgcgacgatc cgaacgacgc
     1021 acagtgcgcc ccgagcgcgc tcaagccgcg gacgccgggc atatggaatc
//
LOCUS       DASUCJ010000286         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_400208, whole genome shotgun sequence.
ACCESSION   DASUCJ010000286 DASUCJ010000000
VERSION     DASUCJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400208"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFU90_03455"
     CDS             <1..822
                     /locus_tag="VFU90_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5478858.1"
                     /translation="VLMAVAAMAAPAAAEVSSIYHGGTYFGSVVVEQGQTVKGDLTVI
                     AGDATVEGVVDGNVDVIGGSFYVRPGGMVTGQVNTIGGDVASAVVPWSAPPERVAPAA
                     DYRILWRIASGLIAVLFFLIFPLRTRIALDRLERHPGLCAAVGLLGWVAVLPVALLLL
                     CTIVLIPFIGLEAVAIVAAIFLGKAALALLVGRRLCELVKPATTPAPLVALIAGLILI
                     TAAELVPIVGTLVTVFVFLIGMGAAILAFTGEPLVGSPVAQPSRPPLSGPPMPAA"
     gene            832..>1823
                     /locus_tag="VFU90_03460"
     CDS             832..>1823
                     /locus_tag="VFU90_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5478859.1"
                     /translation="MTTVSRAARFAERTAHLRASTIREMLKVTQQPDVISFGGGLPAP
                     ELFPAAEIADAARRVMDRRGAAALQYSVTEGIPEMRRGVAERLTRRMGRIFSADDVTI
                     VNGSQQGLDLVGKIFLDPGDHVVLENPSYLGAIQAFDAYQARYLTVDTDDDGAIPESL
                     ERVLEHADPFPKFLYLVPNFQNPTGRTLSAERRERIVRICERYDLPIVEDDPYGELRF
                     EGANLRALVSYESTAPILYSGTGSKIMAPGMRIAWLVVGDDDIREKIVLAKQGADLHS
                     GTFAQYVFHEYVSDADAFDRHVAAIAATYRVRRDVMAEALRRIGFEGLHCSLPSG"
BASE COUNT          270 a          626 c          592 g          335 t
ORIGIN      
        1 gtactgatgg ccgttgccgc gatggcggcg ccggccgccg ccgaagtttc gagtatctat
       61 cacggcggca cgtacttcgg atcggtcgtc gtcgaacagg gtcagaccgt caagggcgac
      121 cttacggtga ttgccggcga cgccaccgtc gagggggtcg tcgacggcaa cgtcgacgtc
      181 atcggcggca gcttctacgt gcggcccggc ggaatggtca ccgggcaggt caacacgatc
      241 ggcggcgacg tcgccagcgc cgtcgttccg tggtcggcgc cgcccgagcg ggtcgccccc
      301 gccgccgact atcgcattct gtggcgaatc gcttctgggc tgatcgcggt gctgttcttt
      361 ctgatctttc cgttgcgtac gcggattgcg ctcgaccggc tggagcgtca tccggggctg
      421 tgcgcggccg tcggattgct cggatgggtc gcagtcctcc cggtggcgct gctcctgctg
      481 tgcacgatcg tcctgattcc gttcatcggc ctcgaggcgg tcgcgatcgt cgccgccatc
      541 ttcttaggaa aagccgcgct ggcgctgctg gtcggacgcc ggctgtgcga actggtgaaa
      601 ccggccacga cccccgctcc gctagtcgcc ttgattgccg gcctgatact cattaccgcc
      661 gccgagctgg ttccgatcgt gggaacgctc gtaacggtat tcgtcttcct gatcggcatg
      721 ggggcggcga tcctggcctt taccggcgaa ccgctcgtcg gctcgcccgt cgcacagccg
      781 tcgcgaccgc cgctgagcgg acccccgatg cccgccgcct aactccccgg gatgaccacc
      841 gtctcccggg cggcccgatt cgcagagcgc accgcgcatt tgcgcgcttc gaccatccgc
      901 gagatgctga aggtcaccca gcagcccgac gtcatctcgt tcggcggcgg tctacccgcg
      961 cccgagctct tcccggccgc cgaaatcgcc gacgcggcgc gccgcgtcat ggaccggcgc
     1021 ggcgctgcgg cgctgcaata cagcgtgacc gaaggcattc ccgagatgcg ccgcggggtg
     1081 gccgagcgcc tcacccgccg gatgggccgc atcttttcgg ccgacgacgt cacgatcgtg
     1141 aacggttcgc agcagggtct cgatctggtc gggaagatct tcctcgatcc cggcgaccac
     1201 gtcgttttgg aaaatccgtc gtatctcgga gccatccaag ccttcgacgc gtatcaggcg
     1261 cgttatctta ccgtcgacac cgacgacgac ggcgcgattc cggagtcgct ggagcgggtc
     1321 ctcgagcacg ccgatccgtt cccgaagttc ctgtacctcg ttccgaactt tcaaaatccg
     1381 accggacgca cgctctcggc cgagcgccgc gaacggatcg tacgcatctg cgaacggtac
     1441 gatctgccga tcgtcgagga cgacccctac ggcgagctgc gtttcgaagg cgctaactta
     1501 cgcgcgctgg tgtcgtacga gagcaccgcg ccgattctgt actccgggac cggcagcaag
     1561 attatggcgc ccggcatgcg gatcgcctgg ctcgtcgtgg gcgacgacga catccgcgag
     1621 aagatcgtgc ttgccaagca aggcgcggat ttgcacagcg gcacgttcgc acagtacgtg
     1681 ttccacgagt acgtcagcga cgcggacgcc ttcgaccggc acgtggccgc gatcgcggcg
     1741 acgtaccgcg tgcgccgcga cgtcatggct gaggcgctcc ggcgcatcgg cttcgaaggg
     1801 ctgcattgtt cgctcccctc cgg
//
LOCUS       DASUCJ010000287         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_446383, whole genome shotgun sequence.
ACCESSION   DASUCJ010000287 DASUCJ010000000
VERSION     DASUCJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446383"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="VFU90_03465"
     CDS             <1..>1156
                     /locus_tag="VFU90_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478860.1"
                     /translation="LGHPAGVGAQIKPVAAGLAVTADGRRIAVANMYNDSLSVVDVPT
                     RHAIANIDLRPGKSGGAHGAPGGNYPYWVAIRGNDTAYVSSVRDREIVVVDLGTMRVA
                     RRIELPGNPNKMLLDRAERRLFVAMDNADAVAIIDTNRNEVTATVGTVAPDGVLEHAK
                     GYGGAAPNSLALSADESTLYVTDGGTNAIAVIDLRERKPEVEGLIPTGWYPQHVVVGG
                     PHSDMLYVVNSKSIPGPNRGNCEGGPRGCPKTSLIKYEYNQYVLQLSRSGFQAMPIPA
                     DARVLAALTKQVEANNTFRFAAGSRDAATMSFLREHVKHVIYIIRENRTYDQLLGDIG
                     KGNGDPTLTQFGEKTTPNQHALAKQFGLYDNVYDVGTNSAEGHNWLMQGD"
BASE COUNT          226 a          372 c          388 g          170 t
ORIGIN      
        1 cgttggggca cccggccggg gtcggggcgc agatcaagcc ggtggcggcg ggactcgcgg
       61 tgaccgccga tggccgccgc atcgccgtcg cgaacatgta caacgactcg ctctcggtgg
      121 tcgacgtacc cacgcgccac gcgatcgcga acatcgatct ccggccgggc aagagcggcg
      181 gtgcgcatgg agcaccgggc ggcaactatc cgtactgggt cgcgattcgc ggcaacgaca
      241 cggcgtacgt ctcgagcgtg cgcgatcggg agatcgtcgt cgtcgatctc gggacgatgc
      301 gcgtcgcgcg gcggatcgag ctccccggaa atcccaacaa gatgctgctc gatcgcgccg
      361 agcgccgtct cttcgtcgcg atggacaacg ccgacgccgt cgccatcatc gataccaatc
      421 gcaacgaggt cacggcgacg gtcggcacgg tggcacccga cggcgtcctc gagcatgcga
      481 agggatacgg cggcgcggcg ccgaacagcc tcgcgctctc ggccgatgaa tcgacgttgt
      541 acgtgaccga cggcggcacg aacgcgatcg cggtcatcga ccttcgagag cgcaagccgg
      601 aagtcgaggg gctgatcccg acgggctggt atccgcagca cgtcgtggtc ggaggtcccc
      661 attcggacat gctctacgtc gtgaacagca agagcatacc ggggccgaac cgggggaatt
      721 gcgagggcgg tccgaggggg tgtccgaaga cgagcttgat caagtacgaa tacaaccagt
      781 acgtgctgca gctctcgcgc tccgggttcc aagcgatgcc gatcccggcc gatgcgcgcg
      841 tgctcgcggc gctcacgaag caggtcgaag cgaacaacac gttccgattc gcggccggct
      901 cccgtgacgc agcgacgatg tcgttcctgc gcgagcacgt gaagcacgtc atctacatca
      961 ttcgcgagaa ccgcacgtac gaccagctgc tcggcgacat cggtaagggc aacggcgacc
     1021 cgacgctcac gcagttcggc gagaagacga cgccgaatca gcacgcgttg gccaagcagt
     1081 tcggtcttta cgacaacgtg tacgacgtcg gcaccaactc tgctgagggc cacaactggc
     1141 tgatgcaagg cgacaa
//
LOCUS       DASUCJ010000288         1223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_858919, whole genome shotgun sequence.
ACCESSION   DASUCJ010000288 DASUCJ010000000
VERSION     DASUCJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858919"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..539)
                     /locus_tag="VFU90_03470"
     CDS             complement(102..539)
                     /locus_tag="VFU90_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU5478861.1"
                     /translation="MRPGWDEYFMEIARTVATRATCPRASVGCVLVREHRILTTGYNG
                     SPRGVPHCTEVGCMLVNDHCMRATHAEANAIVQAALHGVNLSDSTAYCTHQPCVNCSK
                     LLISAGVRKIVYEVAYPDAVAQELLSEAGVGLTAFASLEGLRP"
     gene            complement(546..>1223)
                     /gene="upp"
                     /locus_tag="VFU90_03475"
     CDS             complement(546..>1223)
                     /gene="upp"
                     /locus_tag="VFU90_03475"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5478862.1"
                     /translation="RMAPNERLYVVDHPAVADRLARLRDRDTATPVFRKLLEELGAFL
                     AYEATRALPMRDEDVITPITPMVARRIAVRPVVAPILRAGLGLLPGFLQVIDDAVVAH
                     LGFYRDPQTLQPVPYYANVPHDLSQRHVFVLDPMLATGNSGAAAIEALVTRGATEVTF
                     ISLIAAPEGVRTIHAAHPSVRIVTGSLDERLNDHGYIVPGLGDAGDRMFGSTSSVPVA
                     VRPRENA"
BASE COUNT          242 a          388 c          394 g          199 t
ORIGIN      
        1 catgccgacg tggtgcgcca ggcggatgat gaagggcgtg agcagcgacg agacgacgcc
       61 cgcaagcacc agagccgcta tataaatgta caccggcgcg ttcacggccg cagaccttcc
      121 agggatgcaa acgccgtaag cccgaccccg gcttcggaga gcagttcttg agcgacggcg
      181 tcgggatatg ccacttcgta cacgatcttc cgtacgccgg cgctgatgag cagcttcgag
      241 caattcacgc agggctggtg cgtgcagtac gccgtcgaat cggaaagatt cacgccgtgg
      301 agcgctgcct gcacgatcgc gttcgcctcc gcatgcgtcg cgcgcatgca gtgatcgttg
      361 acgagcatgc agcccacttc cgtgcaatgc ggcactccgc gcggcgagcc gttatagccc
      421 gtcgtcaaga tccggtgttc gcgaacgagc acgcacccaa ccgaagcgcg cgggcatgtc
      481 gcgcgagtcg caaccgtccg cgcaatctcc ataaagtatt catcccaacc ggggcgcatg
      541 ataatttacg cgttctcccg cggccgcacg gcaaccggta ccgaactggt gcttccgaac
      601 atgcgatcgc cggcgtcgcc cagacccggg acgatgtagc cgtgatcgtt cagacgctcg
      661 tcgagcgagc cggtgacgat gcgcacggac ggatgcgccg cgtgaatcgt gcggacgcct
      721 tcgggcgccg cgataagact gatgaacgtt acttcggttg cgccgcgcgt aacgagcgct
      781 tcaattgccg ccgcgcccga gtttccggtt gcgagcatcg gatccagcac gaagacgtgc
      841 cgctgcgaga gatcgtgcgg gacgttcgca taatacggca ccggctgcag cgtctgcgga
      901 tcgcgataga atcccaaatg cgccacgacg gcgtcgtcga tgacttgcag aaagccgggc
      961 agcagaccga ggcccgcacg caagattgga gcgacgaccg gccggacggc gatgcgtctt
     1021 gcgaccatcg gcgttatcgg cgtgatgacg tcttcgtcgc gcatcggcag cgcgcgggtt
     1081 gcctcgtagg ccaagaaagc accgagctcc tcgaggagct tgcgaaagac cggcgtggct
     1141 gtatcgcgat cgcgcaaacg cgcaagacga tccgccacgg ccggatgatc gacgacgtac
     1201 agacgctcgt tcggtgccat gcg
//
LOCUS       DASUCJ010000289         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1317415, whole genome shotgun sequence.
ACCESSION   DASUCJ010000289 DASUCJ010000000
VERSION     DASUCJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317415"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFU90_03480"
     CDS             complement(<1..1063)
                     /locus_tag="VFU90_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478863.1"
                     /translation="MSLRDGMHETDSTRVARTDALNSDVWYDETLVSGDRVYVVGYRY
                     SLPGETANPRDGATEINAFRLANGHFERLTTTFIESWDYYSANNYASRLVDGRLVFYM
                     PFSAMPVYDGYDGAPRPQEPRVPHRLQYDAASNRVVDDGPLFEPGDVFRPFTKPASPM
                     FHSVVRCALPEDGSLQCLTTAVLGDFAREFYVSPTHVYVWTGDRVVMMGLRDGNVSAH
                     SVSGDPSDQFAFHEANAFTMLRLPLSSFDDGGSQSLDGLRTRLGDASYASAERWVGDT
                     FVAAVTPPARPVPMPGPNAPYAGVPVQPGYIVASSLVFFDAGRNGVRTVSINGPVQRI
                     EAAGARALVAVEVAGRRDLR"
BASE COUNT          205 a          378 c          353 g          163 t
ORIGIN      
        1 accgaaggtc gcggcgcccg gcgacctcaa cggcgacgag cgcgcgggct cccgctgctt
       61 cgatccgctg cacgggtccg ttgatcgaca ccgtccgcac accattgcgt cccgcgtcga
      121 agaacaccag tgagctcgcc acgatgtagc cgggctgcac cggcactccc gcgtacggcg
      181 cattgggacc gggcatcggg accgggcgcg ccggcggcgt caccgccgca acgaacgtgt
      241 cgcccaccca acgctcagca gatgcgtacg acgcgtcacc gagtcttgtg cgaagcccgt
      301 cgagtgactg cgaaccgccg tcgtcgaagc tcgagagcgg cagccgcaac atcgtgaacg
      361 cattcgcctc gtggaacgcg aactggtctg acggatctcc gctgacgctg tgcgcggaaa
      421 cgttgccatc gcgcaggccc atcatcacga cgcggtcacc cgtccacacg tacacatgag
      481 tgggcgagac gtagaactcg cgtgcgaagt cgcccaacac ggccgtcgtc aggcactgaa
      541 gcgatccgtc ctcgggcaat gcgcaccgta cgaccgaatg gaacatcggg gacgcaggct
      601 tcgtgaacgg gcggaagaca tcgccgggct cgaacagcgg tccgtcgtca acgacgcggt
      661 tcgacgccgc gtcgtactgc aaccggtgcg gcacgcgcgg ctcttgcgga cgcggtgcgc
      721 cgtcataccc gtcgtatacc ggcatggccg agaacggcat gtagaacacg agccgcccgt
      781 cgacgagccg cgacgcgtag ttgttcgcgc tgtagtaatc ccacgactcg atgaacgtcg
      841 tcgtcagccg ctcgaagtgg ccgttcgcaa gccggaacgc gttgatctcg gtcgcgccgt
      901 cgcgcggatt cgcggtctcc cccggcaatg agtagcgata accgacgaca tacacgcggt
      961 cgcccgagac aagcgtctcg tcgtaccaga catcggagtt cagcgcgtcg gtgcgcgcga
     1021 ctcgcgtcga atcggtctcg tgcatgccgt cgcgcaatga caccgcgtac agccggcctt
     1081 ggcgcaacac gacgagggc
//
LOCUS       DASUCJ010000290         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_461797, whole genome shotgun sequence.
ACCESSION   DASUCJ010000290 DASUCJ010000000
VERSION     DASUCJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461797"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFU90_03485"
     CDS             complement(<1..765)
                     /locus_tag="VFU90_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5478864.1"
                     /translation="MTRRSANAGLTKAIFSRREDPLRMDGKSPEGAPGSRPRTAPRRW
                     LGQVRARVWLLALALLLAAVSAGAALALVSRPASDRTANTVSAFGPAATWPPGVKAAP
                     DFSLRAQDGRRISLAALRGRTVLLTFIDPACRNLCPLEARELNGAVQSSPTAQRPAIV
                     SVNVNPWAETAAMLRADREKWALGSDWRWALGSLRELRPVWRSYRIAVADRTRTLAHV
                     VVHDVSHTEATYVIDRSGHVRALFLWPFSGDAVMRTA"
     gene            831..>1148
                     /locus_tag="VFU90_03490"
     CDS             831..>1148
                     /locus_tag="VFU90_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478865.1"
                     /translation="MRSTFAWKIGGIVLGVAAAGILIGFYIANWLLSGPPTYRPSASA
                     DRVNITIQTVAAVGPTLSPNPDWVSYLVRKPSGTWERSTIWTVPAHSLVHFTVYQFDG
                     DSGL"
BASE COUNT          177 a          377 c          408 g          186 t
ORIGIN      
        1 ggcggtgcgc atgaccgcgt cgccggaaaa cggccagagg aacaacgcgc ggacatggcc
       61 ggagcgatcg atcacgtatg tcgcctcggt gtggctgaca tcgtgcacga cgacgtgcgc
      121 gagcgtgcgt gtccggtcgg cgacggcgat ccgatagctt cgccagaccg ggcgaagctc
      181 acgcagcgag ccgagcgccc agcgccagtc gctcccgagc gcccactttt cacggtcggc
      241 gcggagcatc gcggccgttt cggcccaggg gttgacgttg accgagacga tcgcgggacg
      301 ctgcgccgtc gggctggact ggacggcgcc gttgagctct cgcgcttcga gcgggcagag
      361 gtttcgacag gctgggtcga tgaacgtcaa caggacggtt cggcctcgca gcgcggcgag
      421 cgagatccgg cggccatctt gggcgcggag gctgaagtcc ggcgctgcct tgacgccggg
      481 cggccacgtc gccgcggggc cgaacgccga aaccgtgttc gcggtgcggt cggaagccgg
      541 tcggctgacg agtgcaagcg cggcgccggc ggagactgct gcgagcagaa gggcgagcgc
      601 gagcagccag acccgtgcac ggacttgccc gagccaacgc cgcggcgctg tgcgtggtcg
      661 ggatccgggc gcaccttcgg gagacttgcc atccatccgc agcggatcct ctcggcgcga
      721 gaatatcgcc ttcgtcagcc ccgcgttcgc cgaacgacgc gtcacgggcc tgatgacagt
      781 ttcgtcattt tggtcgcttc gcgaccgcgg cctttgtacg gtcgtccgcg atgcgatcca
      841 ctttcgcgtg gaagatcggt ggcatcgttc tcggagtcgc cgccgccggc atcctcatcg
      901 ggttctacat cgcgaactgg ctgctgagcg gaccgccgac gtatcgcccc agcgcgagcg
      961 ctgaccgcgt caacatcacg atccagacgg ttgctgctgt cgggccgacg ctctcgccca
     1021 atccggattg ggtttcgtac ctcgtgcgga agccgtcggg cacgtgggag cgaagcacga
     1081 tctggacggt gccggcacac tcgctcgtcc acttcaccgt gtaccagttc gacggcgaca
     1141 gcggtctg
//
LOCUS       DASUCJ010000291         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_599085, whole genome shotgun sequence.
ACCESSION   DASUCJ010000291 DASUCJ010000000
VERSION     DASUCJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599085"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="dnaG"
                     /locus_tag="VFU90_03495"
     CDS             complement(<1..1042)
                     /gene="dnaG"
                     /locus_tag="VFU90_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5478866.1"
                     /translation="MRFDQGAIREIHARADIAALIGQYVQLKKRGNDLVGLCPFHGEK
                     TPSFHVHPDRGFFKCFGCGTGGDAIAFVQKIENVPFGDAVRMLAARTGVELEPENPFA
                     ARARTEREAIYDANRIAASFFARTLASDAGKRARAYCAKRGFADDTLAKFTLGYAPDS
                     WDALVEHLRREGVDLAVAATAGLVKSGQRGYYDFYRDRLMIPTYSTTGEVVAFGGRAL
                     GTGEPKYLNTSTTPVYTKGRHLYALNVARRAAQDERTLIVVEGYLDCIALHQAGFPNA
                     VASLGTSFTPEQAAELRKYAEYVYLCFDGDAAGNAAATKAVEIASKAIEHTGSSVRVV
                     LLPPGEDPDTFVR"
     gene            1122..>2342
                     /locus_tag="VFU90_03500"
     CDS             1122..>2342
                     /locus_tag="VFU90_03500"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5478867.1"
                     /translation="MKRIFDPIHHFIELSEAEARLLDLPPLQRLRRLRQLGLAYLAFP
                     SAEHSRFTHALGALAVGTRAFDELVRHGREFFNGDADVAYQRRLVRAALLLHDIGHGP
                     FSHACEAVLGIRHEARTGELLGLTEMGAALAALEVDAGEVLDLIAGNPQTRYPALREL
                     VSGPNLDADRMDYLQRDAYFTGVATGRYDAEQLIASLRLFERGGRMVMGIDSRGVVAL
                     ESFVMARYLMFASVYFHHTTRIFERVLHDVLAELWPNPRALDPIEEFLRWDDFRVLNE
                     LGSRTSEAARALRERIVVYGVAAEFNAERDLRVFEACRSALIDRFGEDNVWADEQTQL
                     LHRLPLGIGQANTVWVGTAGGVVDARQVSDLIAKLTGNAYWRKLFVRRDGADADEARR
                     ICAATIGSVTDRREI"
BASE COUNT          379 a          788 c          778 g          397 t
ORIGIN      
        1 cgcggacgaa cgtatcgggg tcttctcccg gcgggagcag caccacgcga accgacgagc
       61 cggtgtgttc gatggcttta gacgctattt cgaccgcttt ggttgcagcg gcgttgccgg
      121 cggcgtcgcc gtcgaagcag aggtagacgt attcggcgta cttgcgcagt tcggccgctt
      181 gctcgggagt gaacgaggtt ccgagcgacg ccacggcatt ggggaagccg gcctggtgga
      241 gcgcgatgca gtcgagatac ccttcgacga cgatcagcgt gcgctcgtcc tgcgccgcgc
      301 ggcgtgccac gttgagcgcg tacaagtgcc ggcctttggt gtagaccggg gtggtcgagg
      361 tgttgaggta cttcggttcg ccggtgccga gcgcacggcc gccgaacgcc accacttcgc
      421 cggtcgtcga atacgtcggg atcatcagcc ggtcgcggta gaaatcgtaa tagccgcgct
      481 ggccgctctt gaccagcccg gcggtcgcgg cgaccgcgag atccacgcct tcgcgccgga
      541 ggtgttcgac caatgcgtcc cacgagtcgg gcgcgtaccc gagcgtgaac ttcgcgagcg
      601 tgtcgtcggc gaacccgcgt ttggcacagt acgcgcgcgc gcgcttcccg gcgtcggagg
      661 cgagcgtccg ggcgaagaag ctcgccgcga tccggttcgc gtcgtagatc gcctcgcgtt
      721 cggtgcgcgc gcgggcggca aacggattct ccggctcgag ctcgacgccg gtgcgggcgg
      781 ccagcattcg aaccgcgtcg ccgaacggaa cgttttcgat cttttgaacg aacgcgatcg
      841 cgtcgccgcc ggtgccgcac ccgaaacact tgaaaaatcc gcgatccgga tggacgtgga
      901 acgagggcgt cttctcaccg tgaaacgggc acaggccgac gagatcgttg ccgcgcttct
      961 tgagctgcac gtactgaccg ataagcgccg caatatcggc gcgcgcgtgg atctcgcgaa
     1021 tcgcgccctg gtcgaatctc acctagcgaa ccttcttcgc cgcggcggct ttccgttccc
     1081 gaggaacgcc ggtacgccgc cggaaagaaa cgagtcggcc gatgaagcgc atcttcgatc
     1141 cgatccacca tttcatcgag ctttccgagg ccgaggcacg gctgctcgat ctgccgccgc
     1201 tgcaacgctt acggcggctg aggcagctgg gtctggcata cctcgccttt ccgtccgccg
     1261 agcattcacg cttcacccac gcactcgggg cgctggccgt cggaacccgc gctttcgacg
     1321 agctggtacg ccacggccgg gaattcttca acggcgacgc cgacgttgcg taccagcgcc
     1381 gcttggtgcg cgcagccctg ctgctgcacg atatcgggca cggaccgttt agccacgcct
     1441 gcgaagccgt cttgggcatc cgccacgaag cgcgcaccgg cgagttgctc gggctgacgg
     1501 agatgggtgc ggcactggcc gcgctggagg tggacgcggg cgaagtcctc gacctgatcg
     1561 ccggcaaccc gcagacccgc tatcccgcgc tgcgcgaact cgtcagcggg ccaaatctcg
     1621 acgccgaccg catggactac ctccagcgcg acgcctactt caccggcgtg gcgaccggcc
     1681 gctacgacgc cgagcagctc atcgcttcgc tgcggctgtt cgagcgcggc ggccggatgg
     1741 tcatgggcat cgactcgcgc ggcgtggtcg cgctggaatc gttcgtgatg gcgcgctacc
     1801 tgatgttcgc gtcggtgtac ttccatcaca ccacccgcat cttcgagcgc gtcttgcacg
     1861 acgtactggc cgaactctgg ccgaatccgc gcgccctcga tccgatcgag gaatttctgc
     1921 gctgggacga cttccgggta cttaacgaac tgggcagccg tacgagcgaa gcggcgcgcg
     1981 cgctgcgcga acggatcgtg gtctacggcg tcgccgccga gttcaacgcc gaacgcgacc
     2041 tgcgcgtctt cgaagcgtgc cgcagcgccc taatcgaccg gttcggcgaa gacaacgtct
     2101 gggccgacga acaaacgcag ctgctgcatc gtctcccgct ggggatcggc caggccaaca
     2161 ccgtctgggt cggcaccgcc ggcggcgtcg tcgacgcgcg ccaagtctcg gatctgatcg
     2221 ccaagctcac cgggaacgcc tattggcgga agctcttcgt tcgccgcgac ggcgccgacg
     2281 ccgacgaagc gcgccgcatc tgcgccgcaa ccatcggcag cgtaaccgat cggcgggaga
     2341 tc
//
LOCUS       DASUCJ010000292         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_477034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000292 DASUCJ010000000
VERSION     DASUCJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VFU90_03505"
     CDS             complement(<1..1447)
                     /locus_tag="VFU90_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5478868.1"
                     /translation="MSAPAARTRKRAAVQRPRAKTETVAPPASAPAPQHGTAVMAAFN
                     GGRTIPTPINEPIRSYAPGSSERKELKARLRTMSSERIDIPLVIGGREVRTGNTAPTA
                     MPHDHRHVLADSHLADAKHVTNAIDAARTAHREWSRWSLDDRAAVFLRAAELLATTWR
                     ATLNAATMLGQSKTAFQAEIDSACELIDFWRYNPWFAQELYDEQPRSDHSQWNALEYR
                     PLEGFVYAVTPFNFTSIGGNLPTAPALMGNTVVWKPASTAMLSAHYIMKILEAAGLPP
                     GVINLVSGDAASISSQLIGHRDFAGVHFTGSTGVFNGIWKQIGDNVSTYRSYPRIVGE
                     TGGKDFIVAHASADPAALAVAVTRGAFEYQGQKCSAASRVYVPHSLWPDVRDRIAAMI
                     DEIAVGDVADFRNFMGAVIDKKAFAKIGEYVAEAKRTARVIAGGGTNGEEGYFIEPTL
                     VETRDPGFRLLCEEIFGPVVTAYVYPDAKYEE"
     gene            1643..>1882
                     /locus_tag="VFU90_03510"
     CDS             1643..>1882
                     /locus_tag="VFU90_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478869.1"
                     /translation="MTASKVESGGRLLRMFGVVFGLAVIIGNTIAAGILATPGEIAGR
                     LPTPALFFGVWVAGGLYALLGALNLAELGAMLPRSG"
BASE COUNT          276 a          602 c          666 g          338 t
ORIGIN      
        1 tctcctcgta ctttgcgtcg ggatagacgt acgcggtgac gacgggtccg aagatctcct
       61 cgcacagcaa gcggaacccg gggtcgcgcg tctcgacgag cgtcggctcg atgaagtacc
      121 cctcctctcc gttagtccca ccgccggcaa tgacccgcgc ggtgcgcttg gcctcggcga
      181 cgtactcgcc gatcttggcg aacgccttct tgtcgatcac cgcgcccatg aagttgcgga
      241 aatcggccac gtcgcccacg gcgatctcgt cgatcatcgc cgcgatgcgg tcgcgcacgt
      301 ccggccacag cgagtgcggc acgtaaaccc ggctcgccgc cgagcacttc tgtccctggt
      361 attcgaatgc cccgcgcgtc accgcgactg ctaacgcagc cggatcggcc gacgcatgcg
      421 cgacgatgaa gtccttgccg ccggtctcgc cgacgatgcg gggataggac cgataggtgg
      481 aaacgttgtc gccgatctgc ttccagatac cgttgaacac accggtgctg cccgtgaaat
      541 ggacgcccgc gaagtcgcgg tggccgatga gctgcgacga aatcgacgcg gcatcgcccg
      601 acacgagatt gatgaccccc ggggggagac ccgccgcctc gaggatcttc atgatgtagt
      661 gcgccgacag catggcggta ctcgcgggct tccacacgac ggtgttgccc atgagcgctg
      721 gcgcggtggg gaggttgccg ccgatcgacg tgaagttgaa cggcgtcacg gcgtagacga
      781 aaccttccag cgggcggtac tcgagtgcat tccactgcga gtgatcggag cgtggctgtt
      841 cgtcgtacag ctcctgcgcg aaccacgggt tgtagcgcca gaaatcgatc agctcgcacg
      901 ccgaatcgat ctcggcctgg aacgccgtct tggattggcc gagcatcgtg gcggcattga
      961 gcgtcgcgcg ccaggtcgtg gcgagcagct ccgcggcgcg gagaaagacg gcggcgcgat
     1021 cgtcgagcga ccagcgcgac cattcgcgat gcgccgtgcg cgccgcatcg attgcgttag
     1081 tcacatgctt cgcgtcggcc aggtgagagt cggcgagcac gtggcggtgg tcgtgcggca
     1141 tggccgtcgg cgccgtgttg ccggttcgca cctcgcggcc gccgatcacg agcgggatgt
     1201 cgatgcgctc gctgctcatc gtgcggaggc gcgctttgag ctctttccgt tccgacgagc
     1261 cgggcgcgta cgagcgtatc ggctcgttga tcggcgtcgg aatggtgcgt ccgccgttga
     1321 aggcggccat gacggcggtc ccgtgctgcg gcgcgggtgc gctcgccggc ggcgctacgg
     1381 tttcggtctt ggcgcgcggg cgttggacgg cggcgcgctt gcgtgtgcga gcagctggtg
     1441 cggacatggg tacctccgtc gagccaacgg cggacgcgtg tgtccggccg ggcgctaaag
     1501 ataccgcggg gcaaccggtg cggccaacgc ccgggctctc tggccggcgc gcaggcggca
     1561 tcgcatcata gggtcgtgtc gtccccctcg cgcgtccagt cggtcacggt cgcgtcgttc
     1621 gcccattcgt cacccacgag caatgactgc gtccaaggtc gaaagcggcg gacgactgct
     1681 tcgcatgttc ggcgtggtgt tcgggctcgc ggtgatcatc gggaacacga tcgccgcggg
     1741 catcctggcc acgccgggtg agattgcggg acggttgccg acgcctgcgt tgttcttcgg
     1801 cgtgtgggtc gctgggggcc tttacgcgct gctcggtgcg ctcaatctgg cggagctcgg
     1861 cgcgatgctg ccgaggtcgg gc
//
LOCUS       DASUCJ010000293         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1302148, whole genome shotgun sequence.
ACCESSION   DASUCJ010000293 DASUCJ010000000
VERSION     DASUCJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302148"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..435)
                     /locus_tag="VFU90_03515"
     CDS             complement(64..435)
                     /locus_tag="VFU90_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5478870.1"
                     /translation="MNWFFRFIVNAIVLYLIAKYVPGFNHNAGIWTAIIAAIIFGVVN
                     AILGPILRLLTFPLTVVTFGLFSIVVNYILFAITVWVTPGLHTTGEINPWLANLYGAI
                     IMMIVSTIMHQGTRPETPAAS"
     gene            complement(510..2492)
                     /gene="uvrC"
                     /locus_tag="VFU90_03520"
     CDS             complement(510..2492)
                     /gene="uvrC"
                     /locus_tag="VFU90_03520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5478871.1"
                     /translation="MKLEQTLAQIPDVPGVYLMIGREGEILYIGKAVSLRNRVRSYFQ
                     DSAAHHPRTVAMVERVADVRTIVVTNEVEALILEANLIKRHQPPFNVRLRDDKRYPYL
                     KVTNEAFPRVVFTRMVKDDGARYFGPYTNAHGLRELIDLVRLVFPLRTCREPIDGRRK
                     RPCLQYHIKRCLAPCVGYQSEAEYDRMIDEVVLFLEGKQDSLLTRLQGEMTESAEHFN
                     FESAARIRDRIVAVRRVTEGQKVVWKSRLDMDLIAIARARGQACMQVFFVRGGKLIGQ
                     EHFILDGVADHPGSLLVAEFLKQFYTARTASAPEGSGVSAGRIARDIQVPVPVKARAR
                     TPSTSAAVPKEVLVAELPDERPTIEAWLSELKGQRVRILEPQRGVRADYLRLVRQNAE
                     QNLKAFLAHQEVQETAQARALTDLADALELPELPHRIECYDVSNIAGSNPVSSMVVFV
                     EGRAKKSDYRKFKIQYDRGPNDFAMMQETLRRRLRYLRRATDDEEGPLERELARKEKF
                     NKKPDLLLIDGGKGQLSAVVEVLEELDMTGLSVAGLAKEHEWLYLPGQTDPIVLPPNS
                     AALHLVQRVRDEAHRFAVSYHRRRRAKSMVQSALDSLDGVGPVRKKRLLIAFGSAAGI
                     RRAGLDEIAAVKGMTPALAARVKQALDEPAPSTSRR"
     gene            complement(2500..>2740)
                     /locus_tag="VFU90_03525"
     CDS             complement(2500..>2740)
                     /locus_tag="VFU90_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478872.1"
                     /translation="MNLAHFLLWTLLGSLVFCSGLIVVGYTLGDHLDAILPLLHRGGV
                     IVAVAAVELVVAAIVVVRMRARKRARPQRAAEGDS"
BASE COUNT          496 a          886 c          858 g          500 t
ORIGIN      
        1 ggcgacaccg tatcataaca cgccgtgcaa gcccaaggtt tcgggcggcc ggccgttcgc
       61 gggctatgag gccgccggcg tctccgggcg cgttccctgg tgcatgatcg tcgagacgat
      121 catcatgatg atcgcaccgt agaggtttgc aagccaggga ttgatctcgc cggtcgtgtg
      181 caggcccggc gtaacccaaa cggtgattgc aaacagaatg tagttcacga cgatcgagaa
      241 caacccgaac gtcacgaccg tcagcgggaa ggtgagcagc cttaagatcg gcccgaggat
      301 ggcgttgacg accccgaaga tgatcgccgc tatgatcgcc gtccaaatgc cggcgttatg
      361 gttgaacccg ggaacgtact tcgcgatcaa atataagacg atcgcgttga cgatgaagcg
      421 aaagaaccag ttcaacgttg tcccctttct tttttgcgcc gtctgcgcac cagaaggcgt
      481 atcctctcaa gccttcggat ggctctatct caacgcctgc tcgtcgaggg agcgggctcg
      541 tcgagtgctt gtttgacgcg cgccgcgagc gccggcgtca tacccttgac ggcggcgatc
      601 tcgtccaggc cggcgcgccg aattccggcc gccgatccaa acgcgatcag cagccgcttc
      661 ttccgtaccg gtcccacgcc gtcgagtgaa tcgagcgccg attgcaccat cgatttcgca
      721 cgccggcggc gatggtagct cacggcgaag cgatgcgctt cgtcgcggac gcgctggacg
      781 aggtgcagcg ccgccgagtt cggcgggagg acgatcggat ccgtttgtcc cggcaagtac
      841 agccactcgt gctccttcgc caacccggcc accgacaagc ccgtcatgtc gagttcttcg
      901 agcacctcga cgaccgcact caactgcccc ttgccgccgt cgatcaagag caggtcgggc
      961 ttcttgttga acttttcttt gcgcgcgagc tcgcgttcga gcggaccttc ttcgtcgtcg
     1021 gtggcacgcc gcaagtagcg cagccgccgg cgcagcgttt cctgcatcat cgcgaaatcg
     1081 ttggggccgc gatcgtattg aatcttgaac ttgcgatagt cgctcttctt cgcccggcct
     1141 tcgacgaaga cgaccatcga cgacaccgga ttcgagccgg cgatgttcga gacgtcgtag
     1201 cactcgatcc gatgcggcag ctcgggaagt tcgagcgcat cggccaagtc ggtcagcgcg
     1261 cgcgcctgcg ccgtttcctg cacctcttgg tgggctaaga acgccttgag gttttgctcg
     1321 gcattctgcc gcacgagccg cagatagtcg gcccgcacgc cgcgctgcgg ctcgaggatg
     1381 cgcacccgct ggcccttcag ctccgacagc caggcttcga tcgtcggccg ttcgtcggga
     1441 agctcggcga ccagcacttc cttgggcacc gcggcgctgg tcgaaggcgt gcgcgcccgc
     1501 gccttgaccg gaaccggaac ctggatatcg cgcgcgatcc gcccggccga cacgccgctt
     1561 ccctcagggg cgctggccgt acgcgccgtg taaaactgct tgagaaactc ggcgacgagc
     1621 agcgagcccg gatgatcggc gacgccgtcg agaataaagt gctcttgccc gatcagcttt
     1681 ccgccgcgca cgaaaaagac ttgcatgcac gcttgaccgc gcgcgcgggc gatcgcgatc
     1741 aaatccatgt ccaagcgcga cttccagacg accttctggc cttcggtcac ccggcgcacc
     1801 gccacgattc gatcgcggat tcgcgcggcg gactcgaaat tgaagtgctc ggcggactcg
     1861 gtcatctcgc cttgcaggcg cgtgagcagc gaatcctgtt tgccttcgag gaagagcacg
     1921 acctcgtcga tcatccggtc gtactctgct tccgactgat agccgacgca cggcgcgaga
     1981 caccgcttga tgtggtactg caggcacgga cgcttgcgcc ggccgtcgat cggctcgcgg
     2041 caggtacgca gcggaaagac taaacggacg aggtcgatca gctcgcgcag gccgtgcgcg
     2101 ttggtgtacg ggccgaaata gcgcgcgccg tcgtccttga ccatccgggt aaacacgacc
     2161 cgcggaaacg cttcgttggt gaccttcaag tagggatagc gcttgtcgtc gcgcagccgc
     2221 acgttgaacg gcggctgatg ccgcttgatt agattggctt cgaggatcaa cgcctcgacc
     2281 tcgttggtga cgacgatcgt gcgaacgtcg gcgacccgct cgaccatggc caccgtccgg
     2341 gggtggtgcg cggcgctgtc ttggaagtac gagcgcacgc gattgcgtag cgagacggcc
     2401 ttgccgatgt agaggatctc gccttcgcgg ccgatcatca ggtagacgcc cggcacgtcc
     2461 ggaatctggg cgagcgtctg ctcgagcttc accctacctt taactgtcgc cctcggcggc
     2521 gcgttgcgga cgtgcccgct tgcgcgcccg catgcggacc accacgattg ccgccacgac
     2581 caactcaacg gcggcgacgg ccacgatcac gccgccgcgg tggagcaacg ggagaatcgc
     2641 gtcgaggtga tcgccgagcg tgtagccgac gacgattaat ccgctgcaaa acacgagcga
     2701 cccgagcagc gtccacagca agaagtgggc gaggttcatc
//
LOCUS       DASUCJ010000294         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1011862, whole genome shotgun sequence.
ACCESSION   DASUCJ010000294 DASUCJ010000000
VERSION     DASUCJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011862"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFU90_03530"
     CDS             complement(<1..393)
                     /locus_tag="VFU90_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5478873.1"
                     /translation="MEHVAESRSKGFFRSSPSTAFDQYLQDIQKLPLITEPEEERRLA
                     RRAQKGDERAAERLVTANLRFVISYVKKYQGHGLDLSELVAIGNEGLLKAVKKFDPDQ
                     GVKFISYAVWWVRQAVLKALAEQTRSVRI"
     gene            complement(648..824)
                     /locus_tag="VFU90_03535"
     CDS             complement(648..824)
                     /locus_tag="VFU90_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478874.1"
                     /translation="MAPRIFVLAVVICIVGLAFAAYLLDVPPLWIAIAVALVVVRAAF
                     SIWRRARRERIENR"
     gene            complement(846..>1781)
                     /gene="aroB"
                     /locus_tag="VFU90_03540"
     CDS             complement(846..>1781)
                     /gene="aroB"
                     /locus_tag="VFU90_03540"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5478875.1"
                     /translation="VDSFEDPSPLLLRVPPGEVHKTRATWASLTDEMLDAGCGRDTTV
                     VALGGGVVGDLAGFVAATFMRGIPVVQVPTTLLAMIDASLGGKTGVDAPAGKNLVGAF
                     HQPSAVIADPQALATLPLEQRRAGAAEAIKHGVITDTAYFDRVAAALPALLHDPAGDA
                     MHELIIGSITIKAGVVLGDEREHGMRKILNFGHTLGHAIETASDYRLLHGEAIAIGMV
                     LEAALAEMIGVATAGTAPRIRDAVARAGLPTAPPPALSPERLLALTRTDKKARGGAVE
                     YALPLTIGTMAGAETQWGVRVRDDDVLAVLRAASH"
BASE COUNT          293 a          524 c          649 g          315 t
ORIGIN      
        1 aatgcggacg gagcgggttt gctcggcgag ggctttgagg acggcttgtc tgacccacca
       61 gacggcgtac gaaatgaatt tcacgccctg gtccgggtcg aacttcttga cggctttgag
      121 cagcccttcg ttcccgatcg ccaccagctc actgagatcc agaccgtgcc cctggtattt
      181 cttgacgtac gaaatgacga atcgaaggtt ggcggtcacg aggcgttcgg cggcgcgctc
      241 atcacctttt tgagcgcgtc gtgcgagacg tcgttcttct tcgggttcgg ttatgagggg
      301 tagcttctgg atgtcctgta ggtactgatc gaatgcggtg gagggagaag agcggaaaaa
      361 ccctttggac ctgctctcgg ccacatgctc catactcgcc ccgttccttt cgtaaaaggg
      421 tggacgaacc ggaaagcctt gcgtgctcga aaccgccagg ggagcgcacg ataatgtgag
      481 cccccggtta ggtcaacagt cacgaaatcg cagcgtgaga ggtcgttgtg tctttatggg
      541 ctaattgcat tggggcacaa cgatttgcga ctcccatgcg ttttttgcgc gaccgccgga
      601 cccgatggaa cagcgaagct cgcgtgatgt tgcgggctga cggggcgtca gcggttctcg
      661 attcgctcgc ggcgagcacg tcgccagatc gaaaatgcgg cgcgaacgac gacgagtgcg
      721 accgcgatgg cgatccacag cggcgggaca tcgaggaggt acgcggcgaa cgcgagaccc
      781 acgatgcaaa tgacgactgc gagcacgaag atgcgcggtg ccacgctggg actcctcgat
      841 cgaggtcagt gcgatgcggc gcggagcacg gcgagcacgt cgtcgtcgcg gacgcgcacg
      901 ccccactgcg tctcggcgcc ggccatggtg ccgatggtga gcgggagcgc gtattcgacg
      961 gcgccgcctc gcgctttctt atccgtacga gtgagcgcga ggagacgttc cggagacagg
     1021 gcgggcgggg gcgcggtcgg gagcccggcg cgcgccacgg cgtcgcggat gcgcggcgcg
     1081 gtccccgcgg tggcgacacc gatcatttcg gcgagtgcgg cctcgagcac catgccgatc
     1141 gcgattgctt cgccgtggag caggcgatag tcgctcgcgg tctcgatcgc gtggccgagg
     1201 gtgtggccga agttgaggat cttgcgcatg ccgtgctcgc gctcgtcgcc taacacgacg
     1261 ccggccttga tggtgatgct gccgatgatg agctcgtgca tcgcgtcgcc ggcgggatcg
     1321 tggagcagcg cggggagcgc ggcggcgacg cggtcgaaat aggcggtgtc ggtgatgacg
     1381 ccgtgcttga tggcttcggc ggcgccggcg cgccgctgct ccaggggcag tgttgcgagg
     1441 gcctgcgggt cggcgatgac ggcggacggc tggtggaacg cgccgacgag attctttccg
     1501 gcgggcgcgt cgacgccggt cttgccgcct aacgatgcgt cgatcatcgc gagcagcgtc
     1561 gtcggcacct ggacgacggg gatgccgcgc atgaacgtgg cggcgacgaa tcccgcgaga
     1621 tcgccgacca cgccgccgcc gagcgcgacg acggtggtgt cgcggccgca gccggcatcg
     1681 agcatctcgt cggtgagact cgcccaggtg gcgcgtgtct tgtgcacttc gcccggcggg
     1741 acgcgcagca gcagggggga cgggtcttcg aacgagtcga c
//
LOCUS       DASUCJ010000295         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_18521, whole genome shotgun sequence.
ACCESSION   DASUCJ010000295 DASUCJ010000000
VERSION     DASUCJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18521"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFU90_03545"
     CDS             <1..452
                     /locus_tag="VFU90_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478876.1"
                     /translation="LGDKFGRRLQFAADIRYTITEPEFEVESYLRHQADSFVKRFDAN
                     TYLYTSRALTYFDLARQYGGGSLARALAGVSARTLLIAFSSDWLYPPSASDEVAGALR
                     ATGKPAEFHVIEAPYGHDCFLLEEARQTPIIRQFLSRHCPAEGGAGG"
     gene            472..>1169
                     /locus_tag="VFU90_03550"
     CDS             472..>1169
                     /locus_tag="VFU90_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5478877.1"
                     /translation="MTDKPPRADEPHAFGFETRQLHAGQRPDPNTGARAVPIFQTTSY
                     VFEDPESAAAYFNLQEYGNTYSRIMNPTVAVFEERVANLEGGAGAVAFASGISAQAAA
                     LFTLLQPGDHVVSSSALYGGTVNQLKHLLRKMSVELTWVDPDDPDAWRQAVRANTKAF
                     FAETIGNPAGNVLDIETVAAIAHSRELPLIVDNTFATPYLCRPIEWGADIVLHSATKF
                     LGGHGTSIGGVVVEA"
BASE COUNT          193 a          396 c          401 g          179 t
ORIGIN      
        1 cgctgggcga caagttcggc cggcggctgc aattcgccgc cgacatccgc tacacgatca
       61 ccgaaccgga gttcgaggtc gagagctacc tgcgccacca ggccgactcg ttcgtcaagc
      121 gcttcgacgc gaacacctac ctctacacgt cgcgcgcgct gacctatttc gatctcgcgc
      181 gccagtacgg cggcggatcg ctcgcgcggg cgctcgcagg cgtttcggcg cggacgctgc
      241 tgatcgcgtt cagttccgac tggctgtacc cgccatcggc gtcggatgag gtcgccggcg
      301 cgctccgcgc caccggcaag ccggccgagt tccatgtgat cgaggcgccg tacggccacg
      361 actgcttcct gctcgaggaa gcgcggcaga cgccgattat ccgccagttc ctgagcagac
      421 actgcccggc cgaaggcggg gccggcggat gacgtcgagg aagggatctt cgtgacggac
      481 aagccccccc gggccgacga accccacgcg ttcgggttcg agacacggca gctgcacgcg
      541 gggcaacggc ccgacccgaa cacgggcgca cgggcggttc cgatcttcca gacgaccagc
      601 tacgtgttcg aggacccgga atcggcggcg gcgtacttca acctgcagga gtacgggaac
      661 acgtactcgc gcatcatgaa tccgaccgtc gcggtgttcg aggagcgggt ggcgaacctg
      721 gagggcggcg cgggcgcggt ggcgttcgcc agtgggatct ccgcgcaggc cgccgcgctc
      781 ttcacgctgc tgcagccggg cgaccacgtc gtctcgtcct cagcgcttta cggcgggacg
      841 gtgaaccagc tgaagcatct gctgcgcaag atgagcgtgg agctgacgtg ggtcgatccc
      901 gacgatccgg acgcgtggcg gcaggcggtc cgcgcgaaca cgaaggcgtt tttcgccgag
      961 acgatcggta acccggccgg gaacgtgctg gacatcgaga ctgtggcggc catcgcgcac
     1021 tcccgcgagc tgccgctgat cgtggacaac acgttcgcga cgccgtacct gtgccgcccg
     1081 atcgaatggg gcgccgacat cgtgctccac tcggcaacca agttcctcgg cggccatggc
     1141 acgagcattg gcggcgtcgt cgtcgaggc
//
LOCUS       DASUCJ010000296         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1225969, whole genome shotgun sequence.
ACCESSION   DASUCJ010000296 DASUCJ010000000
VERSION     DASUCJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225969"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFU90_03555"
     CDS             <1..412
                     /locus_tag="VFU90_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5478878.1"
                     /translation="RKALGKDGDYAFGMTSWLPSPTLKDRWFGNAEDFEKAWKAQFGF
                     EPDYHAASAVADVEAFAMAIEAAGTADPKKVRDALARIDFESLYGRIHFDSVGQISLP
                     QTVVQVQNGQVVAIYGDKGFINKAEYPMPDWSKR"
     gene            545..>1299
                     /locus_tag="VFU90_03560"
     CDS             545..>1299
                     /locus_tag="VFU90_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5478879.1"
                     /translation="MDLLWQGVVNGVLLGGLYALMALGLSLVWGVLNIVNLAHGALIM
                     LGGYVCYYLFTGAHLDPFAALPITMVVLFVVGYAIQRGLLNLVIRAPMFNTLLITFGI
                     DVVLTYAAQIAFSADFRTINPSYAGSNFLLFGATVPIVRLVAFAIAIGLTVALWLFLG
                     RVPLGRAIRATAQNLVAARLYGVEPRTIYALTFGIGAALAGAAGSLYGVVSQLNPYIG
                     TALTAKSFVIAIIGGLENPLGVIVGGLVLGLVES"
BASE COUNT          198 a          440 c          422 g          239 t
ORIGIN      
        1 ccgcaaggcc ctcggcaagg acggcgacta cgcattcggc atgacatcgt ggctgccgtc
       61 gcccacgctg aaggaccgct ggttcggcaa cgccgaggat ttcgagaagg cgtggaaagc
      121 gcagttcggc ttcgagcccg attaccacgc ggcttcggcg gtcgcggacg tcgaggcatt
      181 cgcgatggcg atcgaggcgg ccggcacggc cgatccgaag aaggtccgtg acgcgctcgc
      241 caggatcgac ttcgagtcgc tctacggtcg catccacttc gacagcgtcg gccagatcag
      301 cttgccgcag accgtcgtcc aggtccagaa tggccaggtg gtcgcaatct acggggacaa
      361 gggtttcatc aacaaggccg agtacccgat gcccgactgg tcgaagcgct gaagaagttg
      421 gcgtgacgcg gcgcgagccg cgccacgtcc gatgacgtaa cgacgcagtc ggagggacgg
      481 tcgcgccgcg ttcggcgcga ccgtcgcctt gtagcaagtc cgcaacggag cgggaggccc
      541 cgcgatggac cttctctggc aaggcgtggt caacggcgtc ctcctgggcg gactctacgc
      601 gctgatggcg ctcggccttt cgctggtctg gggcgtcctc aacatcgtca acctggcgca
      661 cggcgccctg atcatgctgg gcgggtacgt ctgctattac ctgttcaccg gcgcccacct
      721 cgatccattc gcggcgctgc cgatcacgat ggtggtgctg ttcgtcgtcg gctatgcgat
      781 ccagcgcggg ctgctcaatc tggtgattcg cgcgccgatg ttcaacacgc tgctgatcac
      841 gtttgggatc gacgtcgtgc tgacctacgc ggcgcagatc gccttcagcg cggatttccg
      901 cacgatcaac ccgtcctacg cgggctccaa cttcctgctg ttcggcgcaa cggttccgat
      961 cgtgcgcctc gtcgccttcg cgatcgcgat cggcctcacc gtcgcactgt ggctgttcct
     1021 gggccgcgtg ccgctcggcc gggcaatccg cgcgaccgcg cagaacctcg tcgccgcgcg
     1081 actgtacggc gtcgagccgc ggacgatcta tgcgctgacc ttcggcatcg gcgcagcgct
     1141 cgccggcgcc gccggctcgc tgtacggtgt cgtctcgcag ctcaatccct acatcggtac
     1201 agcgctgacc gcgaagtcgt tcgtcatcgc gatcatcggc ggcctcgaga acccgctcgg
     1261 cgtgatcgtc ggcggcctcg tcctcggcct cgtcgaatc
//
LOCUS       DASUCJ010000297         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_889839, whole genome shotgun sequence.
ACCESSION   DASUCJ010000297 DASUCJ010000000
VERSION     DASUCJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889839"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFU90_03565"
     CDS             complement(<1..775)
                     /locus_tag="VFU90_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU5478880.1"
                     /translation="MRTLWRIWPHLAYRLYSNDERKREDFLSTFAPFHLVLLLVVWVA
                     MLVIGWGLFFYGVRDELRPSNVGFASSVYYAGASLLTLGYGDIVATSTFARVMSLVAA
                     SSGLGVVAVVTSFLFAVFGAFQNRETFIVTIGARAGVPPSGLGLLAVHGFAGLRDDLP
                     QVFRDGQTWAAHVMESHLAYPVLMMFRSSHDYESWLGTLGTLLDAAALVITTVEGVNA
                     GQARIMYKIGRHLTHDFARYFHLDVTVSTGIEREEFEHAC"
     gene            complement(901..1350)
                     /locus_tag="VFU90_03570"
     CDS             complement(901..1350)
                     /locus_tag="VFU90_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5478881.1"
                     /translation="MRKLWVLGFVFLLSLGANGGGCSNPNAVGVQQYGTIVGRVLDAT
                     NNQPVGNVLISVGSIYTAYSDPSGAFMLSNIPIGHQAVTANKAGYERNSIDAAVHQDK
                     TTDIGYLRIMPLTGGPTAPPPATPAPTAGPPTPIPAETPTPSASSTP"
BASE COUNT          281 a          462 c          457 g          242 t
ORIGIN      
        1 cgcatgcgtg ctcgaactcc tcgcgttcta ttcccgtcga gaccgtcacg tccaaatgga
       61 aatagcgcgc gaagtcgtgc gtcaaatgac ggccgatctt gtacatgatc cgggcttgcc
      121 cggcgttcac gccctcgacc gtcgtgatga ccagcgcggc cgcgtcgagc agcgtgccca
      181 gcgtgcccag ccacgattca taatcgtgac tcgaacgaaa catcatcagc acggggtatg
      241 cgagatggct ctccatgacg tgcgccgccc acgtttgccc gtcgcggaac acctgtggaa
      301 gatcgtcgcg cagcccggca aagccgtgaa ccgcgagcaa gcctagaccg ctcggcggca
      361 cgcccgcgcg cgcgccgatg gtaacaatga aggtttcgcg gttctggaac gcgccgaaga
      421 ccgcaaacag aaacgacgtc acgaccgcca ccacgcctag cccgctcgat gcggcaacca
      481 gcgacatgac gcgcgcgaac gtcgaggttg cgacgatgtc gccgtacccc aacgtcagca
      541 gcgatgcgcc cgcgtaatag acggacgaag caaaaccgac gttgcttggg cgcagctcgt
      601 cgcgcactcc gtagaagaac agaccccagc cgatcaccag catcgcaacc cagacgacga
      661 gcagcagaac gaggtggaac ggcgcaaacg tgctcagaaa gtcttcgcgc ttgcgttcgt
      721 cgttgctata gagccggtac gccagatgcg gccagatgcg ccacagcgtg cgcatcagaa
      781 aatggctgat gcgcagcgtc gtgttctcgg cgcgcgggac gatgacgcct tgaaagacgt
      841 cgcgcagcgc aacgccgacg atgaagaggc cgccggcgac ggcggcgata tttggcgcgc
      901 ttacggggtg gagctcgcgc tcggcgtagg cgtttccgcg ggaatcggcg tgggcgggcc
      961 ggcggtcggc gcgggcgttg ccggcggcgg tgcggtggga ccgcccgtca gcggcatgat
     1021 gcgcagatag ccgatatccg tcgttttatc ttgatgcacg gcggcgtcga tactgttgcg
     1081 ttcgtagccg gctttgttcg cggtcaccgc ttgatggccg atcggaatat tcgagagcat
     1141 gaacgcaccg ctcggatccg agtacgcggt gtagatcgag ccgaccgaga tcagcacgtt
     1201 tccgacgggc tgattgttgg tcgcgtccag cacgcgtccc acgatcgtgc cgtactgctg
     1261 cacgccgacg gcattcggat tgctgcagcc tcccccgttg gcgcccaggc tcagcaagaa
     1321 gacgaaaccg agaacccaca atttgcgcat atctattcct tcgcgatcaa cgcgccgtgc
     1381 actcgccgcg tgccggtgcc actttggtag cacccttacg cccgctcgcc gcgagtaggg
     1441 gt
//
LOCUS       DASUCJ010000298         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1424505, whole genome shotgun sequence.
ACCESSION   DASUCJ010000298 DASUCJ010000000
VERSION     DASUCJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424505"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFU90_03575"
     CDS             <1..356
                     /locus_tag="VFU90_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5478882.1"
                     /translation="MPGAIKHDYFPHGGANPRDQLVHEVAVAPTSRLAALLEEQGAMV
                     NSMHHQGIKSVPPSLVATAHAPDGLVEGLESNDGAFLFGVQWHPEDLTNADWHMRRLF
                     AGLLDAARAWRGAAR"
     gene            422..889
                     /locus_tag="VFU90_03580"
     CDS             422..889
                     /locus_tag="VFU90_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478883.1"
                     /translation="MRHVVASLLSVALVVGGSPHPGVAATRADDACAVLSPDDIGKTI
                     SWKVGAGTPGKPIPGVLGRCTWTNGDNGRVIVTLADARHASITLQAWEGAGGKDLSGI
                     GTKAVLVKAADFTGGWIVTVEDAKGGFGVSVLGRTATEDNAIALAKIVEGRRK"
     gene            complement(911..1381)
                     /locus_tag="VFU90_03585"
     CDS             complement(911..1381)
                     /locus_tag="VFU90_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5478884.1"
                     /translation="MTQTMIQRVARPAIVLALAAACRTKGGAAPTRSDAPGAIAYPSH
                     IAAGGVAPRGATLTNPYAGDTAAVTAGAALFTAMNCDGCHGGGATGWVGPSLADGRWR
                     YGGAAGEIFSSIYYGRRQGMPAFGGIMPAAGIWKVVTYLQSLEPPNDVPTESWK"
     gene            complement(1419..>2703)
                     /locus_tag="VFU90_03590"
     CDS             complement(1419..>2703)
                     /locus_tag="VFU90_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5478885.1"
                     /translation="IADVSNGETTPMAPFVVKDRVLVGASGGEFGIRGWVKGLDLQAG
                     HLVWTGYNIGADSELLAKPGQFKPFYDHAPNLGASTWPKNAWQTGGAPVWGWMSYDST
                     LDLVYYGTGNPAPYNPEQRAGDNKWTTSVMARRPEDGTLVWAYQFTPHDSWDFDAAAE
                     MVLADLRIAGAMRKVLVHFDKNGFAYTIDRVTGQVLVAQPFVQENWAKRIDLETGRPV
                     VDSSKLTGASRGNVKNICPTLEGGKSPASPVAYSPRTHLFYVSTSNMCMDYQAVPAAH
                     IRGTPYIGANSPYYAGAGGYMGAFVAWDASVGRKVWEIKERWPVWSGVVATAGDVVFY
                     GTLDGWLRAADARTGKILWSFKVGSGVVGNPITYRGPDGKQYLAVYAGIGGDWFLLAG
                     DVRSDDPADVRPPADFAKDLARHTSQGGMVWIFAL"
BASE COUNT          449 a          912 c          893 g          449 t
ORIGIN      
        1 agatgccggg cgcgatcaag cacgactatt tcccgcacgg cggcgccaat ccgcgcgacc
       61 agctggtgca cgaggtcgcg gtcgcgccca cctcgcgcct ggcggcgctg ctcgaggagc
      121 agggcgcgat ggtgaacagc atgcaccacc agggcatcaa gtcggtgcca ccatcgttgg
      181 tcgccaccgc gcacgcaccc gatggactcg tggaagggct cgagtccaac gacggcgcgt
      241 ttctgttcgg cgtgcagtgg catcccgagg atctgacgaa tgccgactgg cacatgcgcc
      301 ggctgttcgc cggtttgctc gacgcggctc gcgcgtggcg cggcgcagcg cggtagctgc
      361 tggcatctac gctcaggtct aacggaatcg cgataacctc aacattgagg acggaggccg
      421 tatgcgtcac gtcgtcgcct cgttgctgtc ggtcgcactc gtcgtcgggg gtagcccgca
      481 tcccggagtc gccgcgaccc gcgcggacga cgcctgcgca gtcctcagcc cggatgacat
      541 cgggaaaacg atatcgtgga aggtcggcgc cggaacgccg ggtaagccga taccgggcgt
      601 cctcggcaga tgcacgtgga cgaacggcga caacgggcgc gtcatcgtca cattggctga
      661 cgctcgccat gcatcgatca cccttcaggc gtgggaaggc gccggcggga aagacctctc
      721 cggcatcggc acgaaggccg tgttggtcaa agctgcggat ttcaccggcg gctggatcgt
      781 gacggtcgag gatgccaagg gtgggttcgg cgtgagcgtc ctcggtcgca cggcgacaga
      841 ggacaacgcc atcgcgctgg cgaaaatcgt cgagggccgt cggaagtagg cctgcgacag
      901 gcgcgcggtt tcatttccag gactcggtcg gcacgtcgtt aggcggctcg agcgattgca
      961 gataggtgac gaccttccag atgccggccg ccggcatgat gccgccgaac gccggcatac
     1021 cctggcgacg cccgtagtag atggaggaga agatctcgcc ggcggcgccg ccatatcgcc
     1081 accgcccatc ggcgagactg ggtccgaccc aaccggtcgc tccgccgccg tggcagccat
     1141 cgcagttcat cgccgtgaac agcgcggcac cagcggtcac ggcggctgtg tcgccggcgt
     1201 atggattcgt gagcgtcgct ccgcgcggcg ccacgccgcc cgccgcaatg tgagagggat
     1261 acgcgatcgc gcctggcgcg tcgcttcgcg ttggcgccgc accgcctttg gttcgacagg
     1321 ccgccgcgag cgcaagcacg atcgctggtc gcgcaacccg ctgtatcatc gtctgcgtca
     1381 tcgggccgtc tcctccgctg cgcctaacga acggggactc agagcgcgaa gatccacacc
     1441 atgccgccct ggctcgtgtg ccgggccaga tccttcgcga agtcggccgg tggacgaacg
     1501 tcggccggat cgtccgaccg cacgtcaccg gcgaggagga accaatcacc gccgatcccg
     1561 gcatacaccg cgaggtattg tttgccgtcc ggtccgcggt acgtaatcgg gttgccgacg
     1621 actcccgatc cgaccttgaa gctccagaga atctttcctg tccgcgcatc ggcggcgcgg
     1681 agccagccgt cgagcgtccc gtagaacacc acgtcgcccg ccgtcgcaac gaccccgctc
     1741 cacaccggcc accgctcctt gatctcccac accttccgcc caacggacgc gtcccacgcc
     1801 acgaacgcgc ccatgtagcc gccggcgccg gcgtagtacg gcgagttggc gccgatgtac
     1861 ggcgtgccgc gaatgtgcgc ggcgggaacc gcctgatagt ccatgcacat gttgctcgtg
     1921 gacacgtaga agaggtgcgt gcgcggcgaa tacgcaacgg gtgaggccgg actcttgccg
     1981 ccttccagcg ttgggcagat gttcttcacg ttgccgcgcg acgcaccggt gagcttgctc
     2041 gagtcgacga caggccgccc ggtctcgaga tcgatgcgct tggcccagtt ctcctgcacg
     2101 aacggttggg cgacgagcac ctgtccggtc acacggtcga tggtgtacgc gaatccgttc
     2161 ttgtcgaaat ggacgagcac tttgcgcatc gcgccggcga tgcgcaggtc ggcgagcacc
     2221 atctcggcgg ctgcatcgaa gtcccaacta tcgtgcggag taaactggta ggcccagacg
     2281 agcgttccgt cctcggggcg ccgcgccatc acgctcgtcg tccacttgtt gtctccggcg
     2341 cgttgctcgg gattgtacgg cgcgggattg cccgtcccgt aatacacgag atcgagcgtc
     2401 gagtcgtacg acatccagcc ccacaccggc gcgcctcccg tctgccaggc gttcttgggc
     2461 cacgtcgacg cgccgaggtt cggggcgtga tcgtaaaacg gtttgaactg gcccggcttg
     2521 gcgagcagct cgctgtcggc tccgatgttg tagccggtcc aaacgagatg gccggcttgc
     2581 aggtccagac ccttgaccca tccgcggatc ccgaattcgc caccggacgc gccgacgagc
     2641 acgcgatctt tcacgacgaa cggcgccatc ggcgtcgtct cgccgttgga cacgtcggcg
     2701 atg
//
LOCUS       DASUCJ010000299         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1180069, whole genome shotgun sequence.
ACCESSION   DASUCJ010000299 DASUCJ010000000
VERSION     DASUCJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180069"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFU90_03595"
     CDS             <1..741
                     /locus_tag="VFU90_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5478886.1"
                     /translation="SNKNDPNDARAVALAALRAPALAEVRREDHVTVLRLLAKAHLDV
                     GRARSRACCRLHALVNELVAGGIRKEVVVSQAETLLARIEPAGVAQHQRLELAGELLD
                     EIRVLDAKLKRSKDRITTAVVASNTTLTDLYGVGPIIAATLIGYSGDIRRFPTVGHYA
                     AYNGTAPIELSSGGRTVHRLSRRGNRTLNHAIHMAAVTQIRNLDSEGRVYYDAKLAAG
                     KTSREALRALKRKISDRVYRQLLMDARR"
     gene            complement(995..>1281)
                     /locus_tag="VFU90_03600"
     CDS             complement(995..>1281)
                     /locus_tag="VFU90_03600"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEU5478887.1"
                     /translation="SAVNPKNLILTLAASTSIAATNLPGTDQAIAFLIYGLIATLGVA
                     APVIVYFTMGARASAILDDLKTWLGRHNAAIMAVIFAVIGAKVLGQGIAG"
BASE COUNT          259 a          425 c          390 g          207 t
ORIGIN      
        1 tcgaacaaga acgatcccaa cgacgcgcga gcggtcgcgc tcgcagcgct gcgcgcgccg
       61 gcactcgctg aagtgcgtcg cgaagaccac gtgacggtgt tgcggttgtt ggcgaaggca
      121 cacctcgatg tcggccgggc ccgatcacgc gcctgttgcc ggctacatgc gctcgtgaat
      181 gagctcgtcg cgggcgggat ccgcaaggaa gtcgttgttt ctcaggccga aacgctcctt
      241 gccaggatcg agccggctgg ggtcgcgcag caccaacgtc tcgagctcgc cggtgagttg
      301 ctcgacgaga ttcgcgtgct cgacgcgaag ctgaagcgct ccaaggaccg gatcacgacc
      361 gcggtcgtcg catcgaacac gacgttgact gacctctatg gcgtcggacc gatcattgcc
      421 gcgacgttga tcggatacag cggcgacatc cgacggtttc cgaccgtcgg ccattacgcc
      481 gcctacaacg gcaccgcgcc gatcgagctc agttccggcg gccgcaccgt gcatcggctc
      541 tcgcgccgcg ggaaccgcac cctcaaccat gcgattcaca tggccgccgt cacccagatc
      601 cgcaacctcg actccgaagg acgcgtctat tacgacgcga aactcgccgc tgggaagacc
      661 agccgcgaag cactgcgcgc gttgaaacgc aagatcagcg accgcgtcta ccgacaacta
      721 ttgatggacg cacgccgata ggtccgggag gacaatcagg gaacgactct tacatccagc
      781 gtgaccggcc aaagccctga acgccggcac ttcggatcaa gtcactcccg gacccgacaa
      841 cacgctacgc ccaacgctcc cgtcgcgtca cgggccgatc tcgcgcgccc gaacacatcc
      901 acggaacagc ctcttgacac aaagaggcct cgattggcac gcgctagagc ggaccgggga
      961 gtccttcata ttggctaagc gcaatgcgcc tcgatcatcc ggcgatgcct tggcccagta
     1021 ccttcgcgcc gatcaccgcg aagatgaccg ccatgatcgc ggcgttgtgg cggccgagcc
     1081 aggtcttgag atcgtcgagg attgccgagg cgcgcgcacc catcgtgaaa tagacgatga
     1141 ccggtgccgc aacacccagc gtcgcgatca gaccgtagat caggaacgca atcgcttgat
     1201 cggttcccgg gaggttcgtc gccgcgatcg aggtcgacgc ggccaatgtg aggatgagat
     1261 ttttcgggtt gactgccgag a
//
LOCUS       DASUCJ010000300         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_538013, whole genome shotgun sequence.
ACCESSION   DASUCJ010000300 DASUCJ010000000
VERSION     DASUCJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538013"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1355)
                     /locus_tag="VFU90_03605"
     CDS             complement(153..1355)
                     /locus_tag="VFU90_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5478888.1"
                     /translation="MQSIERAAVITAAVAALAACSANIGGGSLLSAGNGAPAAIRATQ
                     RALPPNVVRACPVTDASVMHCLALIRTDVGGISKFAGYTPADLQDAYALPSSKNGKGQ
                     VVAVIDAFDDPNVEADLATYRSNFGLPPCTSSNSCFRKVNQLGHTGHLPSPDPGWASE
                     QALDTQIVSAVCPHCSILLVEANTPSASNLAKSVDVAVALGANVVSNSYFGFGLKGMQ
                     YAAYYDHPGTIITASAGDEGYGISEPAGYPTVVAVGGTKLTVEHNKRGWKEIAWTWTG
                     SGCEARLAKPSWQTDTGCKGRTMNDVAAIADPARGVAIYDSYYEKGWIEVGGTSVSSP
                     LIAGVYALAENASSLDAAQSLYAPGASLWDVTLGSNGTCVRKYLCNARTGYDGPTGNG
                     TPNGISAF"
     gene            complement(1406..>1596)
                     /locus_tag="VFU90_03610"
     CDS             complement(1406..>1596)
                     /locus_tag="VFU90_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478889.1"
                     /translation="LQTDVPSRRAAVYRAASRRIWSDVPYIPVYSARRLIVRSSDLRN
                     YRVNPTSTPWWNAWQWDI"
BASE COUNT          284 a          514 c          519 g          279 t
ORIGIN      
        1 accgataaga agaccgtcaa cgacaccgaa atgtcgcgct tcaagaagtt cctggacgac
       61 ctcaaacctt ccgacttcaa ccggtaatcg tcgctcgtcc gtagtaacgc ctcggttact
      121 gcgtcctcgc tccttagtgt cttaagcgac ttctagaagg cggagatgcc gtttggcgtg
      181 ccgttccccg tcgggccgtc gtagccggtg cgggcgttgc agagatactt gcggacgcag
      241 gtgccgttgg agccgagcgt gacgtcccac agcgacgcgc ccggtgcgta cagcgattgc
      301 gccgcgtcga gcgacgaggc gttctccgcg agcgcgtaga ccccggcgat cagcggcgag
      361 ctcacgctcg tgccgccgac ctcgatccag cccttttcgt agtagctgtc gtagatcgcg
      421 acgccacggg cgggatcggc gatcgcggcc acgtcgttca tcgtgcggcc cttgcagccg
      481 gtatcggttt gccagctggg tttggcgagc cgcgcctcgc agccgcttcc ggtccaagtc
      541 caggcaattt ctttccagcc gcgcttgttg tgctcgaccg tgagtttcgt accgccgacc
      601 gccacgacgg tgggatagcc tgccggttcg gagatgccgt agccctcgtc gccggcgctc
      661 gccgtaatga tcgtccccgg atgatcgtaa tacgccgcgt actgcatccc tttgaggcca
      721 aagccgaagt agctgttgct gacgacgttc gcacccagcg ctacggcgac gtcgaccgat
      781 ttcgccagat tcgaagccga cggagtgttc gcttccacga gcagaatcga gcagtgcgga
      841 cagacggccg aaacgatctg cgtgtccagc gcttgctcac tcgcccaccc gggatcgggc
      901 gacggaaggt ggccggtgtg cccgagctga ttgaccttgc gaaaacacga attcgagctg
      961 gtgcacggcg gcaagccgaa atttgaacgg tacgtcgcga gatctgcttc cacgttggga
     1021 tcgtcgaacg catcgatgac ggcgacgact tgccctttac cgttcttcga cgacggcagc
     1081 gcgtaggcgt cctgcagatc ggccggcgta taccccgcga acttcgatat tccgccgacg
     1141 tcggtgcgaa tcagcgccaa gcaatgcatg accgacgcgt ccgtgacggg gcaggcgcgc
     1201 acgacgttcg gcggaagcgc gcgctgcgtc gcacggatcg cggccggggc gccgttgccg
     1261 gccgacagaa gcgagccccc gccgatattg gcggagcacg ccgcgagagc cgcgaccgcg
     1321 gcggtgataa ccgccgcccg ttcgatcgat tgcacgagag gaagccctcc aagaagaccg
     1381 aggctcgaga attcgcgcgg acgcgctaaa tatcccactg ccacgcgttc caccacggcg
     1441 tggacgtggg attgacgcga tagttgcgca ggtcggacga acggacgatc aggcggcgcg
     1501 cggagtagac cgggatgtac ggcacgtccg accaaatgcg ccggctggcg gcccggtata
     1561 cggcggctcg ccgcgacggg acgtcggtct gcagcc
//
LOCUS       DASUCJ010000301         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1271886, whole genome shotgun sequence.
ACCESSION   DASUCJ010000301 DASUCJ010000000
VERSION     DASUCJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271886"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFU90_03615"
     CDS             complement(<1..249)
                     /locus_tag="VFU90_03615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5478890.1"
                     /translation="MTGEPERSRVIRTVGEFTSGLTRWFAAHRQLANVAISGEVSDLH
                     DGRSGHLSFKLKEEQAVLECVAWADKRRSIPEFKNRTAV"
     gene            complement(246..2393)
                     /locus_tag="VFU90_03620"
     CDS             complement(246..2393)
                     /locus_tag="VFU90_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5478891.1"
                     /translation="MPKRNGGKGWGVLRGVLVALLLIVLFAAGTIAGMVAAYARNLPD
                     ISRMADYQPASSTRIFARDGTPLASVFKENRVWVPIGRIPPIVRDAFIANEDHNFYNH
                     HGVDFGGIVRAGFADLTHQEFQGASTITQQLARRLFLTDEVSISRKVQEALLAMEIER
                     FYTKDEILERYLNIIYLGAGAYGVDAAAHTYFARPVEKLTLAQAALLAGVVAAPSDYS
                     PFTNMGLARDRERHVLDRMVQSGFITQAQSDEAYAEPLELAGERATGLQGYAYPYFTT
                     YAIAQLEKTFGKRDVEQGGLQVYTTLDPRLQRLAQEAVTWGVGQALGEGINAHQAALV
                     ALRPSTGEIVAMVGGTGFSLANQFNRAWQAHRQPGSSFKAYVYTAAIDSGLTASATMD
                     DTPVSYPMGDGTRWSPYDDDGSYMGTVSLRTALALSRNVVAVKLAERIGVDKVIEYAH
                     RMGITAPLEANLSLALGSSVVSVLDQASGYSTLANQGLHVEPSPFRLVKDSLGNVVLD
                     NRYPQASDVISAGTAYVMTTMLEDVISKGTGYPNAVIGRPAAGKTGTTSNFRDAWFVG
                     YTPDLVTAVWLGNDDYSRMYESYGGNVPARIWARFMKAALAGTPKHDFAFPTDEVAKM
                     RSCGGGGYEYYLKGTEPATPCGQADAAYAPANAVPYATLPPYASLPPAPTLPPTASPD
                     SAATDTPADPTAAPATAPPQNSVIVSTPPPKRR"
     gene            complement(2380..2796)
                     /gene="nusB"
                     /locus_tag="VFU90_03625"
     CDS             complement(2380..2796)
                     /gene="nusB"
                     /locus_tag="VFU90_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5478892.1"
                     /translation="MPSRRLGREQALQALYSIAVGDRDPAEALTEVIGESVGSSHRTF
                     VRDLVLGTIDFRSQADVILGPLLEGWSVERLPTIDRLLLEMGTFELRGKSATPPAVAI
                     NEAVELAKRFSTEDSGRFVNGVLNAVAKGQVVDAQA"
     gene            complement(2816..>3653)
                     /locus_tag="VFU90_03630"
     CDS             complement(2816..>3653)
                     /locus_tag="VFU90_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478893.1"
                     /translation="TDAEDFSRAFQLARSEALSAFGSGDVYVEKYLARPRHIEFQILG
                     DKFGKVIHLGERDCSVQRRHQKLIEESPSPAMTPELRQKMGDAAVAGAKSIDYVGAGT
                     IEMLLDEDGSYYFMEMNTRIQVEHPVTEMVYGIDLVKEQIRIASGEALGYGQSDLVAR
                     GHAIECRVNAEDPRNNFAPQAGTVSDVVFPGGPGIRVDTHLYAGASIPPYYDSMLAKV
                     VAYGDTRDSAIARMERALSETVIDGVHTTIALSEEILTTPQFRSGRYDIGFLPAMLSS
                     AV"
BASE COUNT          596 a         1227 c         1173 g          657 t
ORIGIN      
        1 gacggcggtt cggttcttga actccggaat gctgcggcgt ttgtccgccc acgcgacgca
       61 ctcgaggacc gcctgttctt ccttgagttt gaagctcagg tggccgctgc gcccgtcgtg
      121 aaggtccgag acctcgcccg atatcgcgac gttcgcaagc tgacggtgcg ccgcgaacca
      181 gcgcgtaagg ccgctggtaa actcgccgac cgtgcggatg acgcgcgagc gttcgggctc
      241 gccggtcatc gtcgtttcgg cggcggcgtc gacacgatta cggaattttg cggcggcgcc
      301 gtcgccggcg cagcggtggg atccgcgggc gtatcggtcg ccgcgctgtc gggtgacgcg
      361 gtcggcggaa gggtcggcgc cggcggcaag gacgcgtacg gcggcagcgt tgcgtacgga
      421 acggcattcg ccggcgcgta cgccgcgtcg gcttgtccgc agggcgttgc gggctcggtc
      481 cccttcaaat aatattcgta accgccgccg ccgcacgagc gcatcttcgc cacttcgtcg
      541 gtgggaaacg cgaagtcgtg tttcggcgtg ccggccagcg ccgctttcat gaagcgcgcc
      601 caaatccgcg ccgggacgtt cccgccgtac gattcgtaca ttcgcgaata gtcgtcgttg
      661 ccaagccaca ccgcggtcac caagtccggc gtgtagccga cgaaccacgc gtcgcgaaaa
      721 ttcgacgtcg tccccgtctt gccggcggcc ggacgcccga tcaccgcgtt ggggtaaccg
      781 gttcccttcg agatcacgtc ttcgagcatc gtcgtcatga cgtacgcggt gccggcgctg
      841 atcacgtcgc tggcctgcgg atagcggttg tcgagcacca cgttgccgag cgaatccttg
      901 accagccgga acggcgacgg ttcgacgtgc agtccctgat tggccaatgt cgagtacccg
      961 ctggcctgat cgagcaccga gacgaccgac gagccgagcg cgagcgacag attcgcttcc
     1021 agcggggcgg tgattcccat ccggtgcgcg tattcgatca ccttgtcgac gccgatgcgt
     1081 tcggccagct ttaccgccac gacgttgcgc gacagcgcga gcgcggtgcg cagcgacacc
     1141 gttcccatgt acgagccgtc gtcgtcgtac ggcgaccagc gcgttccgtc gcccatcgga
     1201 tagctgaccg gcgtgtcgtc catcgtggcc gacgcggtga gccccgaatc gatcgccgcg
     1261 gtgtagacgt acgccttgaa cgacgaaccc ggctgacggt gcgcctgcca ggcgcgattg
     1321 aactgattgg ccagcgaaaa tccggtaccg ccgaccatcg ctacgatctc gccggtcgac
     1381 ggccgcaacg ctaccaaggc ggcctgatgg gcgttgatcc cttcgccgag cgcttgtccc
     1441 acgccccacg tcaccgcctc ctgcgcgagg cgctgcaagc ggggatcgag cgtcgtgtac
     1501 acctgcagcc cgccttgctc gacgtcgcgt ttgccgaacg ttttctcgag ttgcgcgatc
     1561 gcgtaggtcg tgaagtacgg ataggcgtag ccctgcaaac cggtcgcgcg ttcgccggcc
     1621 aattcgagcg gttcggcgta ggcttcgtcg gattgcgcct gggtgatgaa tccgctctgg
     1681 accatccggt ccagcacgtg ccgctcgcgg tcgcgcgcca gccccatgtt cgtaaacggc
     1741 gagtagtcgg agggcgcggc caccacgccc gccagcagcg cggcttgcgc cagcgtgagt
     1801 ttttcgaccg gccgcgcgaa gtaggtatgc gcggcggcgt cgacgccgta agcgcccgct
     1861 ccgagataga tgatgttgag atagcgctcg aggatttcgt ctttggtgta gaaccgctcg
     1921 atctccatcg ccagcagcgc ttcttggacc ttgcgcgaga tcgacacttc gtcggtcaga
     1981 aaaagccgcc gcgcgagctg ctgcgtgatc gtcgacgccc cttgaaactc ctggtgcgtc
     2041 aaatcggcga acccggcacg cacgatcccg ccgaagtcca cgccgtggtg gttgtagaag
     2101 ttgtggtctt cgttggcgat gaacgcgtcg cgcacgatcg gcggaatgcg cccgatcggc
     2161 acccatacgc ggttttcttt gaacaccgac gccagcggcg tgccgtcgcg cgcgaagatc
     2221 cgcgtcgagc tggccggctg gtagtcggcc atccggctga tgtccggcag gttgcgcgcg
     2281 tacgcggcca ccattccggc gatcgtcccg gcggcgaaca gcacgatcag cagcagcgcg
     2341 accagcacgc cgcgcagaac gccccagccc ttacctccgt tacgcttggg catcgaccac
     2401 ctggcccttg gcgaccgcgt tgagcacgcc gttgacgaac cggccggagt cttcggtcga
     2461 aaagcgcttg gccagctcga ccgcttcgtt gatggcgacc gccggcggcg tcgcggactt
     2521 cccgcgcagt tcgaaagtac ccatctctaa gagcaaacga tcgatcgtcg gcaagcgttc
     2581 gacgctccag ccctccagca gcggcccgag aatcacgtcg gcttgcgagc ggaagtcgat
     2641 cgtgccgagg acgagatcgc gcacgaaggt gcggtggctc gatcccacgc tttcgccgat
     2701 aacttcggtc agcgcctcgg cgggatcgcg atcgcccacc gcaatcgaat acagcgcctg
     2761 cagtgcctgt tctcgcccca atcgccggct cggcatcgcg tttcccagcg aacctctaga
     2821 cggccgagct cagcatggcc gggaggaatc cgatatcgta ccgcccggag cgaaattgcg
     2881 gcgtcgtcaa gatctcctcg ctcaacgcaa tcgtcgtgtg cacgccgtcg atcaccgtct
     2941 cgctcagcgc gcgttccatc cgcgcgattg ccgagtcgcg cgtgtcgccg taggcgacga
     3001 ccttggcgag catcgagtcg tagtacggcg gtatgctcgc cccggcatag aggtgagtgt
     3061 cgacgcggat ccccggaccg cccggaaaaa cgacgtccga aaccgtaccc gcctgcggcg
     3121 cgaagttgtt gcgcggatct tcggcgttga cgcggcattc gatcgcgtgg ccgcgggcca
     3181 ccaggtcgga ctgcccgtag cccagcgcct cgcccgacgc gatccgaatc tgttccttga
     3241 cgaggtcgat tccgtacacc atctcggtca ccggatgctc gacctgaatg cgcgtgttca
     3301 tctccatgaa gtagtacgag ccatcctcgt cgaggagcat ctcgatcgtg ccggcgccga
     3361 cgtagtcgat cgacttcgcg ccggcgacgg cggcgtcgcc catcttctgg cgaagctcgg
     3421 gagtcatcgc cggactcggg ctctcctcga tcagcttctg gtggcggcgc tgcaccgagc
     3481 agtcgcgctc accaagatgg atcaccttgc cgaacttgtc gcccaggatc tggaactcga
     3541 tgtggcgcgg ccgcgcgaga tacttctcga cgtacacatc accgctgccg aaggccgata
     3601 gcgcctctga tcgcgccagc tggaaggccc gcgagaaatc ctcggcatcg gtc
//
LOCUS       DASUCJ010000302         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_156060, whole genome shotgun sequence.
ACCESSION   DASUCJ010000302 DASUCJ010000000
VERSION     DASUCJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156060"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFU90_03635"
     CDS             <1..246
                     /locus_tag="VFU90_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5478894.1"
                     /translation="RDVMAQLEARGAGHIYVDGGVTIQRFLEAGLIDHLIVTRVPVLI
                     GSGIPLFGPLTRDIVVRHVATRQFGGGLVQSEYDVVR"
     gene            complement(348..791)
                     /locus_tag="VFU90_03640"
     CDS             complement(348..791)
                     /locus_tag="VFU90_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478895.1"
                     /translation="MTGTYDASAEPQLVLAGSGYGLAGDYSAANGVIIGTSTFPNGKT
                     GLWTVSTDAAAVKVFCGTYTSTQGNGGGTWNLVLDSKNNLTGVATVAGQLQGTLSGTS
                     LSVTYTGGTASGTLDPSTGAGSGTYDAPNIGQGDAGDWTANTGGC"
BASE COUNT          188 a          383 c          393 g          209 t
ORIGIN      
        1 cgcgatgtca tggcgcaact ggaagcgcgc ggcgccgggc acatctacgt ggacggcggc
       61 gtcaccatcc agcgatttct cgaggccggc ctgatcgatc atctcatcgt cacgcgcgtg
      121 ccggtgctca tcggctcggg catcccgctc ttcggaccgc tcacgcgcga cattgtcgtt
      181 aggcacgtgg cgacgcgcca gttcggcggc ggcctcgtcc agagcgaata cgacgtcgtg
      241 cgatagcggc gtcggcgcac tgatggccta acgctcgatg cccgggtgcg gcgaggtgga
      301 cgcgcagggc ggcgcaagcc gccgccgtgc gcgtttcgag gaaaaaatca gcagccgccc
      361 gtattggccg tccaatcgcc tgcgtcgccc tgaccgatgt tgggcgcatc gtacgttccg
      421 ctgccggcgc ccgtggacgg atcgagtgtg cccgaagccg taccgcccgt gtacgtcacc
      481 gagagcgacg tgcccgacag cgtaccttgt agttggccgg cgaccgtggc gacacccgtc
      541 aggttgttct tcgagtcgag cacgaggttc caagtgccgc cgccgttccc ctgggtgctg
      601 gtgtacgtgc cgcagaacac cttcaccgct gcggcgtcgg tcgagaccgt ccagagtccg
      661 gtcttgccgt tggggaaggt ggaagttccg atgatcacac cgttcgccgc ggagtagtcg
      721 ccggcgagtc cgtatccgct gccggcaagt acgagctgcg gctcggcgct cgcgtcgtac
      781 gtgccggtca tcgcgatgac ttcgccgcct ggcgtgagcg taagcgtgcc cgtgacctgt
      841 tcgactgtgc cagcctcagc aacctgcacc aatgaaaatc ccgacggacg ctgggatcgg
      901 ctggcgaccg atgccgggaa agtaatggtc aacactccgc tcgcgccgtc gcccacgagc
      961 gtgcccttga attctcccgt tggtccggtg gacggcgacg tcgagctcga cccgccgcat
     1021 gcgacggacg caaggatgac ggttgcggca agccatcgca tgagcgccgc tggccggatt
     1081 gcgagaacgt gtgactgggc tgtttgcatt tgagctcccc gttggaaaga ccagcgaccg
     1141 gacgtcttgg cgaatgggac cccacattcg agc
//
LOCUS       DASUCJ010000303         1458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1485713, whole genome shotgun sequence.
ACCESSION   DASUCJ010000303 DASUCJ010000000
VERSION     DASUCJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485713"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFU90_03645"
     CDS             <1..271
                     /locus_tag="VFU90_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478896.1"
                     /translation="WMPNGSAIVYRDRSRYYRVPVATKGGFHTGKPTLLIEGSFLSAF
                     AWNHAMSPDGRVLVLMNSPDRDARTLETITNFQSLVARTAHAGRR"
     gene            complement(326..>1458)
                     /locus_tag="VFU90_03650"
     CDS             complement(326..>1458)
                     /locus_tag="VFU90_03650"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5478897.1"
                     /translation="YGNVSDAASKLAQLSGNQSPLLALISIASQNTNTDTTIGNALQP
                     AHAVVPPGQTDKLIGQSVQGYMAALLTLQGSLGQVATAPPGQSEGAIANATQNANAAR
                     GEAQKLAQTFVVGREPAVATAVQRLLTEPLASIDPYLRNYGAGQLNGKGESFCARNAT
                     LFTKYPFNERSPIQASLDEVSTVFKPGTGSLWTYYNDALQNVIQAQGSTFAPKAGGTL
                     NVNPAFLSFFNRAAQFSAAIFPAGSNEPRLTLNLTNYLMPQSGRLRVVGDGLTVDYAA
                     AVKPHQFTWTFQPSGEATIAVEQGSTWANVASFHGTWAVFQLFGAAASWEPTATDYRA
                     EWSIPGNGAGAKVGLDVGLNNVPPVLRKGFFAGFSCVSRVVQ"
BASE COUNT          226 a          471 c          499 g          262 t
ORIGIN      
        1 ctggatgcct aacggtagcg cgatcgtgta tcgcgatcgc tcgcgctact accgcgtgcc
       61 cgtcgccacg aagggcggct tccataccgg gaagccgacg ctgctcatcg aagggtcgtt
      121 tctgtcggca ttcgcgtgga accacgcgat gagtccggat ggtcgggtgc tcgtgctcat
      181 gaacagtccc gatcgagacg cgcggacgct ggagacgatc acgaatttcc agtcgctggt
      241 ggcgcgcacg gcgcacgcgg gccgtcgtta ggacaagctc cgtcgtgccg cggaccaccg
      301 ctgaacgtgt ccggcgatgg gcggtctact gcaccacgcg gctcacgcac gagaacccgg
      361 caaagaatcc tttccgcagc accggcggca cgttgttcag gccgacgtcg agcccgacct
      421 tcgcaccggc gccgttgccg gggatgctcc attcggcgcg atagtcggtg gccgtcggtt
      481 cccacgaggc cgcggcgccg aacaactgga acaccgccca cgtgccgtgg aagctggcga
      541 cgttcgccca ggtactgcct tgctcgaccg cgatggtcgc ttcgcccgac ggttggaacg
      601 tccacgtgaa ttggtgcggc ttgaccgcgg cggcgtagtc caccgtcagt ccatcgccca
      661 ccacgcgcag gcgtccgctc tgcggcatca ggtaattggt gagattgagc gttaggcggg
      721 gctcgttgga gccggccggg aaaatcgccg ccgagaattg cgcagcgcgg ttgaagaacg
      781 agaggaaggc cggattgacg ttcagcgtgc cgcccgcctt cggcgcgaac gtgctgccct
      841 gggcctgaat cacgttctgg agcgcatcgt tgtaataggt ccacaacgac cccgtgcccg
      901 gcttgaagac cgtgctcacc tcatcgagcg acgcctgaat cggcgaccgc tcgttgaacg
      961 gatacttcgt gaagagcgtc gcgttcctgg cgcagaacga ttcgcccttt ccgttcagct
     1021 ggccggcgcc gtagttgcgc agatagggat cgatgctggc cagcggttcg gtgagcaggc
     1081 gctgcacggc ggtcgccacc gcgggctcgc gcccgacgac gaaggtctgc gccagcttct
     1141 gcgcttcgcc gcgcgcggca ttggcgttct gcgtcgcgtt ggcgatggcg ccttcggact
     1201 gcccgggcgg cgccgtcgcc acctggccta acgaaccctg cagggtgagc agcgcggcca
     1261 tgtatccctg caccgactgg ccgatcaatt tgtccgtctg gccgggcggc acgacggcgt
     1321 gcgccggctg cagcgcgttg ccgatcgtcg tatccgtgtt cgtgttctgc gacgcgatcg
     1381 agatcagggc gaggaggggc gactggttgc cggacagttg ggcgagcttg gatgcggcgt
     1441 cgctcacgtt gccgtacc
//
LOCUS       DASUCJ010000304         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_782555, whole genome shotgun sequence.
ACCESSION   DASUCJ010000304 DASUCJ010000000
VERSION     DASUCJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782555"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFU90_03655"
     CDS             complement(<1..451)
                     /locus_tag="VFU90_03655"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU5478898.1"
                     /translation="MPGLDTNVAYLKGVGPRRAEALHRLGLRTARDLLYHLPHRYHDA
                     STIARISTLTPGMDATVIGTVISKGIIPTRKGLRIFQAVLKDRTGLIEAAWPGQPFLD
                     RVVNKDDVLLVTGRVRFFHGRQLQPREMVNLGPAGDTDEVTGGRVLAV"
     gene            complement(517..>1044)
                     /locus_tag="VFU90_03660"
     CDS             complement(517..>1044)
                     /locus_tag="VFU90_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HEU5478899.1"
                     /translation="GKQVLVAAGDTYRAGAIDQLRVWADRVGAQFVGSAPGSDPAAVA
                     YNAIDSAVARRADVVIVDTAGRLHTQDDLMTELRKVARVIGKRLPGAPHETLLVLDGT
                     IGQNAVAQARSFSASVPLTGVVITKLDGTAKGGIVVAVHEALDVPIKFVGVGEKADDL
                     VPFDAAQYARDLFAE"
BASE COUNT          177 a          361 c          348 g          158 t
ORIGIN      
        1 acaccgcgag cacgcggccc cccgtcactt cgtccgtgtc gccggcggga ccgaggttca
       61 ccatctcccg cggctgcagc tgtcgtccat gaaagaatcg gacgcggccc gtgacgagca
      121 acacgtcgtc cttgttcacg acgcgatcga gaaacggctg gccgggccac gcggcttcga
      181 tcaagccggt acggtccttc aacaccgcct ggaagatccg aagacctttg cgcgtgggaa
      241 tgatgccttt cgagatcacg gtgccgatca ccgtcgcgtc catgcccggc gtgagcgtcg
      301 agatgcgcgc gatggtactg gcgtcgtgat agcgatgcgg caggtggtac aacaggtcgc
      361 gcgccgtgcg gagcccaagc cggtgcagcg cttcggcgcg gcgggggccc acgcccttca
      421 ggtacgcgac gttggtatcg aggcccggca cgggttaggc gaagaaagcg gacgcggccg
      481 gacacggccg gagatgatcg gagccaaacg gtcaggttac tcggcgaaca gatcgcgggc
      541 gtactgcgcg gcgtcgaacg gcaccaggtc atcggccttc tcgccgacgc cgacgaactt
      601 gatggggaca tcgagcgctt cgtgcacggc caccacaatg ccgcccttcg cggtcccatc
      661 gagcttggtg atcacgacgc ccgtgagcgg caccgatgcc gagaacgagc gcgcctgcgc
      721 caccgcgttc tgcccgatgg tgccgtccag cacgagcagc gtctcgtgcg gcgcgcccgg
      781 caaccgcttg ccgatcacgc gcgcgacttt ccgcaattcc gtcatgaggt cgtcttgcgt
      841 gtgcagacgg cccgcggtat ccacgatcac gacgtcggcg cggcgcgcca ccgccgaatc
      901 gatcgcattg taggcgacgg cggcgggatc gctacccggc gcgcttccga cgaactgcgc
      961 acccacacgg tcggcccaca cgcgcaactg gtcgatggcg ccggcgcgat acgtgtcgcc
     1021 ggccgcaacc agcacctgct tgcc
//
LOCUS       DASUCJ010000305         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_950848, whole genome shotgun sequence.
ACCESSION   DASUCJ010000305 DASUCJ010000000
VERSION     DASUCJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950848"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="dapF"
                     /locus_tag="VFU90_03665"
     CDS             complement(<1..593)
                     /gene="dapF"
                     /locus_tag="VFU90_03665"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5478900.1"
                     /translation="MRKLEFVKMHGCGNDYIYVVADRVRPADPAALAMRLSDRRFGIG
                     GDGLIMLCPSERADVRMEMYNADGSRGEMCGNGIRCVARLHYETSATRRNPITVDTDC
                     GIKTITLTLEDGRVVAATVDMGEPTLEGREIPVDIDGRVIGHPLQVAGRLEKITAISM
                     GNPHCVVFVNDESVFALDDADFARLGRQFEHHPFFPRR"
     gene            complement(586..>1198)
                     /locus_tag="VFU90_03670"
     CDS             complement(586..>1198)
                     /locus_tag="VFU90_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478901.1"
                     /translation="IVKGLRADGIALKYLDLGGGLGIPYQEEPPPPSEYATALLGPLR
                     GIGLKIIIEPGRVLVGNAGILVTKVLYVKETDVRRFIIVDGAMNDLIRPVLYEAFHDI
                     RPVIQRRGGRIVTADVVGPVCESGDFFARERDLPEAHSGDLLAVMSAGAYGFVMASNY
                     NSRPRAAEVLVDGAEFHVVRERETFEDLIRGEKIVTLKSVPDA"
BASE COUNT          215 a          369 c          402 g          212 t
ORIGIN      
        1 cggcgcggga agaaggggtg gtgttcgaac tggcggccga gccgggcaaa gtccgcgtcg
       61 tcgagcgcga agacactctc gtcgttgacg aagacgacgc aatgcgggtt gcccatcgag
      121 atcgcggtaa ttttctcgag gcgtcccgcg acctgcagcg gatggccgat cacgcgcccg
      181 tcgatgtcga cgggtatttc acgcccttcg agcgtcggtt cgcccatgtc gacggttgcg
      241 gcgaccacgc ggccgtcttc gagcgtaagg gtgatggtct tgatgccgca gtcggtgtcg
      301 acggtaatcg gattcctgcg cgtcgcggag gtttcgtaat ggaggcgcgc gacacaacga
      361 atgccgttgc cgcacatttc gccgcggctg ccgtcggcgt tgtacatttc catccgcacg
      421 tccgcgcgct cggacgggca aagcatgata aggccatcgc ccccgatgcc gaagcgacgg
      481 tcggaaagac gcatcgcgag cgcggccgga tcggccgggc gcacgcggtc agcgacaacg
      541 tagatatagt cgttgccgca tccatgcatt ttgacgaact cgagcttacg catcgggaac
      601 gctcttgagc gtcacgatct tctcgccgcg aatcaggtcc tcgaaggtct cgcgctcgcg
      661 cacgacgtga aattcggcgc cgtcgaccaa cacctcggcg gcgcgcgggc ggctgttgta
      721 gttcgacgcc atcacgaagc cgtaggcgcc ggcgctcata accgcgagca ggtcgccgct
      781 gtgggcctcg ggcaggtccc gctcgcgcgc gaagaaatcg ccgctttcgc acaccgggcc
      841 gacgacgtcg gcggtaacga tgcggccgcc gcggcgttga atgacgggtc tgatatcgtg
      901 gaaggcttcg tagagcaccg ggcgaatcag atcgttcatc gcgccatcga cgatgatgaa
      961 acgccggacg tcagtttcct tcacgtagag aaccttggtg accaggatgc cggcattgcc
     1021 gaccagtacc cggcccggtt cgatgatgat cttgagaccg ataccgcgca gaggtccgag
     1081 cagcgcggtg gcgtactcgg agggcggcgg cggttcctcc tggtaaggaa tgcccagacc
     1141 gccgccgaga tccagatact tgagcgcgat gccgtcggcg cgcagtccct tgacgatg
//
LOCUS       DASUCJ010000306         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1118846, whole genome shotgun sequence.
ACCESSION   DASUCJ010000306 DASUCJ010000000
VERSION     DASUCJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118846"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1002)
                     /locus_tag="VFU90_03675"
     CDS             complement(25..1002)
                     /locus_tag="VFU90_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478902.1"
                     /translation="MRLLFARFGISIFALAGTLALAACATGGGAGIPIPAPDGVPAVY
                     RATQGVQAPALFVASDSPYFVDGYTLPLHTGERASMMLRGINEPVPVAADSRSLYIGS
                     FDDGSIYTYGLPLEMTWQVPARSPGLATGLRDLSGLAVAGQYLFAAGEGPNGEEVLEY
                     RLPLVAGEKATSFVRGFPVLDFLAVGAERGTLYIASAADGTVGAYRLPLRNGETPEYT
                     IDTPPQIDGAVGVAIDRRNAHLYVSLYRIGDVYRYNLPYRAGEKPVILDVRAQTGGEL
                     PYGIAVGASHLYVTAGAILAYSLPINARSTPDATVSFAGGNAAGLAVSR"
     gene            complement(1158..>1906)
                     /locus_tag="VFU90_03680"
     CDS             complement(1158..>1906)
                     /locus_tag="VFU90_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478903.1"
                     /translation="EEESLDLDMVTAVCPNSHIVLVEANTATKAELGTAENQAISQGA
                     NVVSNSYGGGETGASNSNYNHPGHIITASAGDSGYGAQQPCSYSTVVCIGGTSLSRAN
                     NARGWSETAWSGSGSGCSRYVSKPSWQTDKGCTKRSESDVSADANPNTGVAVYDSYAY
                     QGYQGWLVFGGTSVSSPLIASVYALAGNAASLNYAQSIWAAGGNAAELNDVTSGSNGR
                     CPRQYPYICRAGTGYDGPTGWGTPNGIGAL"
BASE COUNT          328 a          629 c          624 g          325 t
ORIGIN      
        1 ccacggccaa cctgaggata tcgtctagcg gctgacggcc agcccggcag cgttgccgcc
       61 ggcaaaagat acggttgcgt ccggggtgct gcgcgcgttt atcggcaagc tataggcgag
      121 aatcgccccc gccgttacgt ataggtggct cgcgccgacg gcgatcccgt agggcagttc
      181 tcccccggtc tgtgcgcgca cgtcgagtat gaccggcttt tcgccggcgc ggtacggcaa
      241 gttgtagcgg tagacgtcgc cgatgcgata gagcgaaacg tacaggtgcg cgttccttcg
      301 atcgatggca acgccgacag cgccgtcgat ctgtggcggc gtatctatcg tgtactccgg
      361 agtctcgccg ttgcgcagcg gcaatcgata cgcgccgacg gtgccgtcgg ccgccgacgc
      421 gatgtacagc gtcccgcgct cggcgcctac cgctaggaaa tcgagcaccg ggaatcctcg
      481 cacaaagctc gttgctttct cgccggcaac caagggcaga cgatattcaa gcacctcttc
      541 accattgggg ccttcgcccg cagcaaacaa gtactggccg gccaccgcga gtcccgacag
      601 gtcgcgcaac cccgtcgcca gaccgggcga acgggccggc acctgccacg tcatctcgag
      661 gggaagcccg tacgtgtaga tcgagccatc gtcgaagctt ccgatgtaga ggctgcggct
      721 atcggccgca accgggacgg gctcgttgat accgcgcagc atcatcgacg cgcgctcgcc
      781 ggtgtgcagc ggcagcgtat agccgtcgac gaaatacggc gagtccgacg ctacgaaaag
      841 cgccggcgct tgaacgcctt gcgttgcccg atagacggcg ggcacgccgt cgggcgccgg
      901 gatcgggatg cccgctccgc cgcccgtcgc acacgccgcg agcgcgagcg tgccggccaa
      961 cgcgaaaatc gaaatgccga agcgagcaaa gagcaggcgc attcaactca attgtgcacg
     1021 gctgaccggc ggaccgaatc gcgcatcggg cccgaaagcg atgggactaa cgacctaggg
     1081 gtcctatatg ccggccagct taaagaaacg gggcgcggcc gtggccgcgc cccgaagctc
     1141 tccgatcggt taggcgctca aagggcgccg atcccgttgg gcgtccccca gccggtcggc
     1201 ccatcgtagc ccgtacccgc cctgcagatg tacggatact ggcgcgggca gcgaccgttg
     1261 ctgcccgagg taacgtcgtt gagttcggcc gcgttgccgc cggccgccca aatcgactgc
     1321 gcgtagttga gcgacgccgc gttgccggcc agtgcgtaca ccgacgcaat caacggcgag
     1381 ctgacgctgg tgccgccgaa gacgagccag ccctggtaac cctgatacgc gtagctgtcg
     1441 tagacggcta cgccggtgtt cgggttggca tcggccgaaa cgtcggattc cgaacgcttc
     1501 gtgcagccct tgtcggtttg ccacgacggc ttgctgacgt agcggctgca gccgctgccg
     1561 ctgcccgacc atgccgtttc gctccagccg cgcgcgttgt tggcccggga gagcgacgtg
     1621 ccgccgatgc acaccacggt cgagtacgag cacggctgtt gcgcgccgta gccgctgtcg
     1681 cccgcgctcg cggtgatgat atgccccgga tggttgtagt tggagttcga cgcaccggtc
     1741 tcgccgccgc cgtaggagtt gctcacgacg ttggcgccct gcgagatcgc ctgattttca
     1801 gccgtgccga gttcggcttt ggtcgctgtg ttggcttcga ccagcacgat atggctgttg
     1861 ggacaaacgg ccgtgaccat atccagatcc agcgactctt cttcgg
//
LOCUS       DASUCJ010000307         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_568986, whole genome shotgun sequence.
ACCESSION   DASUCJ010000307 DASUCJ010000000
VERSION     DASUCJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568986"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..865)
                     /locus_tag="VFU90_03685"
     CDS             complement(155..865)
                     /locus_tag="VFU90_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5478904.1"
                     /translation="MPRRARLRLAGIPLHVVQRGNNRLPCFLAPDDYPYFLDHLMLFA
                     DRFGCAVHAYALMTNHVHLLVTPGADGGVSTMMKHLSQRYVQHVNRVHGRTGTLWEGR
                     YKSCFVLDETYVLTCYRYIDLNPVRAGLVRHPADYEWTSYRANAEGSCSRSLVPHDAY
                     LGLGRSRDDRLRAYRALVAAEPEDESLQRIRDATTGNVSVGTPATLTAIQNRIGRRVT
                     RGKPGRPRRRAAAPATTT"
BASE COUNT          217 a          363 c          374 g          182 t
ORIGIN      
        1 gggttgatca gaaaaagtcc ttgccacgca aggggttgca cgcgaaatcg ctaccaaaac
       61 tgtcgccggg cggcgacgca agcccgaaaa accgggacgt accacgtttt ccgttggggc
      121 cccgagatcg gcgttcgaag cgacggcaat cgggtcaggt ggtcgtcgcc ggcgccgccg
      181 cccgacgccg cggccgccca ggctttcctc gcgtgacacg acgtccgatg cggttctgga
      241 tcgcggtgag cgtcgccgga gtccccacgg agacgttgcc cgtggtggcg tcgcgaatgc
      301 gctgcagtga ctcgtcttcc ggctccgccg cgaccagcgc tcggtatgcc cgcagacggt
      361 cgtcccggct gcgccccagg ccgagatacg cgtcgtgcgg cacgagtgac ctggagcagg
      421 atccctctgc gttcgcccga tagctcgtcc actcgtaatc ggccggatga cggacaagac
      481 cagcccgcac gggattgaga tcgatgtacc ggtagcaggt gagcacgtag gtctcgtcga
      541 gcacgaagca cgacttgtac cgcccttccc acagcgttcc ggttcgccca tgcacgcgat
      601 tgacgtgctg gacgtaacgc tggctgagat gcttcatcat cgttgacacg ccgccgtcgg
      661 cgcccggcgt gaccagcaga tgaacgtgat tggtcatcag cgcgtacgca tggacggcgc
      721 agccgaaacg atccgcgaag agcatcaggt ggtcgagaaa atacgggtaa tcgtccggcg
      781 cgaggaaaca cggcaaccgg ttattgccgc gctgaacgac gtgaagcgga attccggcaa
      841 ggcgcaggcg ggcacgacgc ggcatgggca cgattcccgg cggggaaatg atggcagacg
      901 cgaggcagcg cgatttcctc gcgccgatcg tgccaaggtt cctcgacgcg aacgggttgc
      961 gccatcatcc gttccgccga attcgagctc cggaaaacgt ggtacgtccc ggttttatgc
     1021 gccaaatgcc cggtccgctg cgtcgagcgt cgcggcgaca tccccgtccg aatgcgccgc
     1081 cgagacgaac cccgcctcgt aagcggaagg cgcgaaatag atgccggcat cgagca
//
LOCUS       DASUCJ010000308         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_966246, whole genome shotgun sequence.
ACCESSION   DASUCJ010000308 DASUCJ010000000
VERSION     DASUCJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966246"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="leuB"
                     /locus_tag="VFU90_03690"
     CDS             <1..961
                     /gene="leuB"
                     /locus_tag="VFU90_03690"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5478905.1"
                     /translation="VGGAALKRGLAAFPDETRALCDRSEAILFGSVGLPEYDGKPLLE
                     RPEYALFLLRRDYELYANLRPVRVFPGLEEASSLKPELVRGLDLVVVRELTGGIYYGR
                     PKEQRMAGGVEEAVDTMIYRAPEIERIARVAFELARTRAKRLTSVDKQNILETSRLWR
                     RVVERVAGDYPDVAVEHLLVDNAAMQLVRRPADFDVIVTENMFGDILSDEAAILTGSI
                     GTLPSASLGTRGTPGKRFGLYEPVGGTAPDIAGKGIANPTAAILSAAMLLRHSLDDEA
                     GALRIERAVERAYAGGARTADLAHDPARALSTAAFAQAVTAQL"
     gene            958..1965
                     /locus_tag="VFU90_03695"
     CDS             958..1965
                     /locus_tag="VFU90_03695"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU5478906.1"
                     /translation="MIEPPTTQTIVSAPARIAGLGVYAPERVVTNADFEKRLDTSDEW
                     IVKRTGIHRRHAVAEDQYSSHLAIAAVEDLLAHHPGIRLETIDYLIVGSSTPDYAYPS
                     LSAMVQAHFRLARTVGALDISTACAGFAYGINLAAGLIGTRQAARVLVIVADALTRSV
                     DFGDRSTCVLFGDGSGAALVEYSAEPRIFGMDAGADGNGGKFLYRTGLRSDINGVVDS
                     TRLLRQEGAAVYRWVLENVPATVRRVLARAGMTLGDVDWFVPHSANLRMIEALDKRLA
                     FPIERTLLSVEDYGNTSAVSIPLALTPAVRDGRVKAGDTVLIVGFGGGLVTAGNVVIW
                     S"
     gene            complement(1973..>2203)
                     /locus_tag="VFU90_03700"
     CDS             complement(1973..>2203)
                     /locus_tag="VFU90_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478907.1"
                     /translation="SVVMGFDLRGDTTLIVSLDLQSYDEMDDDVASAMLRSAVERWKG
                     AWLQAHPHEHAVLHVRFIDFIGRVIAERSANV"
BASE COUNT          363 a          725 c          750 g          365 t
ORIGIN      
        1 cgtgggcggc gcggcgctga agcgcggcct ggcggcattc cccgacgaga cgcgggcgct
       61 gtgcgatcgc agcgaggcga tcctgttcgg cagcgtcggg cttcccgagt acgacggcaa
      121 gccgctgctc gaacgtccgg aatacgcgct gttcttgctt cgccgcgact acgagctgta
      181 cgcgaacctt cgtccggttc gcgtgttccc cggcctcgag gaagcgtcgt cgctgaagcc
      241 cgagctggtg cgcggcctcg acttggtggt cgtgcgcgag ctcaccggcg gcatctacta
      301 cggccggccg aaggaacagc ggatggccgg cggcgtcgaa gaagccgtcg acacgatgat
      361 ctatcgcgca cccgagatcg aacgcatcgc acgcgtcgcc ttcgaactgg cgcgaacccg
      421 cgccaagcgc ctcacctcgg tcgacaagca gaacattctc gaaacgtcgc ggctgtggcg
      481 gcgggtcgtc gagcgcgtgg ccggcgacta ccccgacgtc gccgtcgagc atctgctggt
      541 cgacaatgcg gcgatgcagc tggtgcggcg gccggcggat ttcgacgtga tcgtcaccga
      601 gaatatgttc ggcgacattc tctccgacga ggcggcgata ctcaccggat cgatcggaac
      661 gctcccgagc gcgagcctcg gaacgcgcgg cacgcccgga aaacgtttcg gactctacga
      721 gccggtcggg gggaccgcac ccgacatcgc gggcaagggg atcgccaacc cgaccgccgc
      781 gatcttgtcg gcggcgatgc tgctgcgcca cagcctcgac gacgaggccg gtgccttgcg
      841 catcgagcgc gcggtcgagc gcgcgtacgc cggcggcgcg cgcaccgccg acctggcgca
      901 cgatcccgcg cgagcgcttt cgacggcggc gttcgcgcaa gcggtgaccg cgcagctatg
      961 atcgagccgc cgacgactca aacgatcgtc tcggcgcccg ccagaatcgc ggggctgggc
     1021 gtctacgcac ccgaacgcgt ggtcaccaac gccgacttcg aaaagcggct cgacaccagc
     1081 gacgagtgga tcgtcaagcg cacgggcatt caccggcgcc acgcggtcgc cgaagatcag
     1141 tactcgagcc acctggcgat cgcggcggtc gaggatttgc tcgcgcacca tcccggtatc
     1201 cggctcgaga cgattgacta cctgatcgtc gggtcgtcga cgcccgatta cgcctacccc
     1261 agtctttcgg cgatggtgca ggcacacttt cggctggcgc ggacggtcgg cgcgctcgac
     1321 atcagcacgg cctgcgccgg tttcgcgtac ggcatcaatt tggccgccgg attgatcggt
     1381 acgcggcaag ccgcgcgcgt gctcgtgatc gttgccgacg cgctgacgcg gtcggtcgat
     1441 ttcggcgatc gctcgacctg cgtgctattc ggcgacggct ccggtgcggc gctcgtcgaa
     1501 tacagcgccg aaccgaggat cttcggaatg gatgcgggcg ccgacggcaa cggcggaaaa
     1561 tttctctatc gaaccgggtt gcgttcggac atcaacggcg tggtcgattc gacccggctg
     1621 ctgcgtcagg aaggcgcggc ggtgtaccga tgggtgctcg aaaacgtgcc ggcaacggtt
     1681 cggcgggtgc tggcgcgcgc cggcatgacg ctcggcgacg tcgactggtt cgtgccgcac
     1741 agcgcgaacc tgcgcatgat cgaagcgctc gacaagcgtc tggcctttcc gatcgaacgg
     1801 acgctgttga gcgtcgagga ttacggtaac acgtcggcgg taagcatccc gctcgcgctc
     1861 actccggctg tccgcgacgg ccgggtgaaa gccggcgata ccgtgttgat cgtcggtttc
     1921 ggcggcggtt tggtcaccgc cggcaacgtc gtgatctgga gctgagagtc ttttagacgt
     1981 tcgccgagcg ttcggcgatc acgcggccga tgaaatcgat gaaacgcacg tggagcacgg
     2041 cgtgctcgtg cggatgcgcc tgcagccacg cacccttcca gcgttcgact gcggaacgca
     2101 gcatcgccga cgccacgtcg tcgtccattt cgtcgtagct ctgcagatcg aggctgacga
     2161 tcagcgtcgt atcgccgcgc aggtcgaatc ccatgacgac cga
//
LOCUS       DASUCJ010000309         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_874595, whole genome shotgun sequence.
ACCESSION   DASUCJ010000309 DASUCJ010000000
VERSION     DASUCJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874595"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFU90_03705"
     CDS             complement(<1..236)
                     /locus_tag="VFU90_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5478908.1"
                     /translation="MTFLAGYRLLDLAWFGPGPFCARILGDLGFDVIKITEVDSGRAR
                     PAPALFHESSAKRTDVSYRQANARSMQLNLKTDAG"
     gene            complement(273..>1317)
                     /locus_tag="VFU90_03710"
     CDS             complement(273..>1317)
                     /locus_tag="VFU90_03710"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5478909.1"
                     /translation="PDRSLQNHAGWAGNGSITLNLKLPAARDLALRLVAISDIAAENF
                     GPGVMDAFDLGYDAMCAVKPDIISMSMPAAGLSGPLKGVRTYGMSLSSITGLDSMTGY
                     VGGGPLPFENAFADPLGGVVGAFGALVALNHRKRTGKGQHVDYSQQEGVMQMVGPALM
                     DYFMNGRVAKPMGNRHPLRVGAPHGVFPCAGDDRWISIAVLTDEEWGNFVEAMGSPQW
                     ASAPEFATATARVENIDALHERVAAWTSTQDDRALAGMLQTHGVAAAPVLNVADLLHD
                     PHYRTRGTFVEVRHPLGFDETIYGAYVKTSTTEAAVRPGPCIGQDNEHVFLDLLGLPE
                     DEYRRLIAEEVIY"
BASE COUNT          197 a          431 c          460 g          229 t
ORIGIN      
        1 ccagcgtccg tctttaggtt gagttgcatc gagcgcgcgt tggcctggcg atagctgaca
       61 tccgtccgct tcgccgacga ctcgtggaac agtgccggcg ccggccgcgc gcgaccactg
      121 tcgacctctg tgatcttgat cacgtcgaaa cccaggtcgc cgaggatgcg tgcgcagaac
      181 ggcccggggc cgaaccacgc gagatcgagc agtctgtagc cagcgaggaa ggtcatagca
      241 ttcccggcga tgataccggc gccacggggg cgctaataga tcacctcctc ggcaatcagc
      301 cgccggtatt cgtcttcggg caggccgagc aggtcgagga agacgtgttc gttgtcctgc
      361 cctatgcacg ggccggggcg caccgccgcc tcggtggtgc tcgtcttgac gtaggcgccg
      421 tagatcgtct cgtcgaagcc gagcggatgc cgcacttcga cgaatgtccc gcgggtgcgg
      481 tagtgcggat cgtggagcag gtctgcgacg ttgaggacgg gcgcggccgc gacgccgtgc
      541 gtctgcagca tgccggcgag cgcgcggtcg tcctgcgtac tcgtccatgc ggcgacccgc
      601 tcgtggagcg cgtcgatgtt ctcgacgcgt gcggtcgcgg tcgcgaactc gggagcgctc
      661 gcccactgcg gcgatcccat cgcctcgacg aagttccccc attcttcgtc ggtcagcacg
      721 gcgatgctga tccagcggtc gtcgcccgcg caggggaaga cgccgtgcgg cgccccgacg
      781 cgcagcgggt gccggttgcc catcggtttg gcgacgcggc cgttcatgaa gtagtccatg
      841 agcgccgggc cgaccatctg catcacgcct tcctgctgcg agtagtcgac gtgctggccc
      901 ttgccggtgc gcttccgatg gttcagcgcg acgagtgcgc cgaacgcgcc gacgacgccg
      961 ccgagcggat ccgcgaacgc attctcgaat ggcagcgggc cgccgccgac gtagccggtc
     1021 atgctgtcga gtccggtgat gctgctcagg ctcatgccgt aggtacggac gcccttgagc
     1081 ggtccggaga gccctgccgc cggcatggac atcgagatga tatccggctt caccgcgcac
     1141 atcgcgtcgt acccgagatc gaacgcatcc atgacgccgg gaccgaagtt ctccgcggcg
     1201 atgtcggaaa tggcgacgag gcggagcgcg agatctcgtg ccgcgggcag cttgaggttc
     1261 agcgtgatgc tgccgttgcc cgcccatccg gcgtggttct gcaggctgcg gtcgggg
//
LOCUS       DASUCJ010000310         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_263219, whole genome shotgun sequence.
ACCESSION   DASUCJ010000310 DASUCJ010000000
VERSION     DASUCJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263219"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU90_03715"
     CDS             complement(<1..234)
                     /locus_tag="VFU90_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478910.1"
                     /translation="MIAPTRAACETIEIAIGLHLETLLEREHGGEIRGLARAGKGFGI
                     IAGTGTGKTLAIRPMAETILGTALRVGVVNRERE"
     gene            complement(378..869)
                     /locus_tag="VFU90_03720"
     CDS             complement(378..869)
                     /locus_tag="VFU90_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478911.1"
                     /translation="MTRFALTLLLALAAPLAAQTAEHGKTGDESVIAHRPPPGMCRIW
                     IDGVPAERQPAPTDCATAIRRRPSNARVIFGEELRPPRGQAQPPRLVEQPPKPVEPAP
                     NVTQGNEPKANDPKANEPRTIDPNTNQPRPRVQRPPPRMPPRQVPKPHKPSGPPMSHI
                     RIH"
     gene            complement(1072..2151)
                     /locus_tag="VFU90_03725"
     CDS             complement(1072..2151)
                     /locus_tag="VFU90_03725"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5478912.1"
                     /translation="MRIAISTGGGDAPGLNAVIRAAVLSAIGRGWEVLGVKRGYAGLL
                     GEDEVVPLTRESVRGIAHLGGTILRTTNRGDPFRYPGIAVDGTYCEIDRSAELIERAR
                     NLGIDALITIGGDGSLRIAQRLFERGMKVVGVPKTIDNDVSGTITTFGFDTAVNTALE
                     AIDKLHTTAESHDRVIVMEVMGRDAGFIALHSGLAGTADVILIPEIPYEIEKVCDKIR
                     SRDRAGRYFSIVVVAEGARPLGGSVSVLGPSLPGEELRLGGMADKLAREIQARTGKEC
                     RSLVLGHLQRGGMPTGYDRLLATRFGGAAVRAIAAGCFGQMVALQSPHIVCIPIAEAL
                     AVPKRVDPMHDVVMTARATGISFGD"
     gene            complement(2190..2432)
                     /locus_tag="VFU90_03730"
     CDS             complement(2190..2432)
                     /locus_tag="VFU90_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2400..2402),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HEU5478913.1"
                     /translation="MTITIEYCTLUNYEPRATRLAAELEARYPDASITMQPSRGGRFE
                     VSLDGRPIFEKSKLGRHAKEGEVLGLIERARAERAG"
BASE COUNT          411 a          913 c          809 g          448 t
ORIGIN      
        1 ctctcgctct cgattcacga cgcccacgcg caaggccgtg cctaaaatgg tttcggccat
       61 cgggcggatt gcgagcgtct tccccgtgcc cgtccccgcg atgataccga atcccttgcc
      121 ggcgcgcgcc aagccccgaa tttcgccgcc gtgctcgcgc tcgagcagcg tctcgaggtg
      181 cagtccgatc gcgatctcga tcgtctcaca cgcggcgcgt gtgggagcga tcacgatcac
      241 gcggcccgtc gccggttcgg ccgcgacgaa cgcagcgtga tcgggcggta gcccgtggtc
      301 ctgcggccgg cggaatcgcg gtgaatcact tgccacgtgc gaagcttgtg gcaagcgcga
      361 agcggaggga agccgcgcta gtgaatccgg atgtgcgaca tcggcggacc gctcggcttg
      421 tgcggcttcg gcacttgccg aggcggcatg cgcggcggcg gccgctgcac gcgcggacgc
      481 ggttgattcg tgttcggatc gatcgtgcgc ggctcgttcg cttttggatc gttcgccttg
      541 ggctcgttgc cctgcgtcac gttaggagcc ggctcgaccg gcttcggcgg ctgctccacg
      601 agccgcggcg gctgcgcctg tccgcgaggc gggcgcaact cctcgccgaa aatgacccgc
      661 gcgttggacg gacgccggcg aatcgccgtc gcgcaatccg tcggcgccgg ctgccgctcc
      721 gcgggaacgc cgtcgatcca gatgcggcac attcccggcg gcggccggtg cgcgatcacg
      781 ctctcatcgc cagttttccc atgctccgcc gtctgcgcag caagcggcgc agccagggcg
      841 aggaggaggg tcaaggcgaa gcgggtcatt gtatctccga acctgcgggt ctcacggcac
      901 ctcgggcctg cagaggcaat gccatgattc ccgcgattct aatctcttgt aacaactagc
      961 gacacaacat cttacgaatt ggcagcggca tggtccacga ggcggtgtcc acatgtccgg
     1021 acatgtccgt ccgaccggct ggactgacct ctgccgcggc acgcgccggt ttcagtcgcc
     1081 gaacgagatg cccgtcgccc gcgccgtcat caccacgtcg tgcatcgggt cgactcgttt
     1141 gggcacggct agcgcctcgg cgatgggaat acacacaatg tgcggcgact gcaacgccac
     1201 catctgcccg aagcatccgg ccgcaatcgc gcggaccgct gccccgccga agcgcgtcgc
     1261 gagcagcctg tcgtacccgg taggcatgcc gccgcgctgc agatggccaa gcaccagcga
     1321 ccgacattcc ttccccgtcc gcgcctggat ctcgcgcgcc agcttgtcgg ccatcccgcc
     1381 taaccgaagc tcctcgcccg gcaacgatgg tcccagcacc gacacggagc cgccgagcgg
     1441 ccgcgccccc tctgcaacga cgacgatcga aaagtagcga ccggcacgat cgcgcgatcg
     1501 gattttgtcg caaacctttt cgatctcgta ggggatttcc ggaatgagaa tcacatcggc
     1561 cgtgcccgcc agcccgctgt gcagcgcgat gaaccctgcg tcgcgcccca tcacttccat
     1621 gacgatcacg cgatcgtgac tctcggcagt ggtgtgcagc ttgtcgatcg cctcgagcgc
     1681 cgtgttcacg gcggtgtcga atccgaaggt cgtgatcgtg ccgctcacgt cattgtcgat
     1741 cgtcttgggg acgccgacca ctttcattcc ccgctcgaac aatcgttggg cgatgcgcag
     1801 cgagccgtcg ccgccgatcg tgatcaacgc gtcgatgccg aggttgcgcg ctcgttcgat
     1861 gagctccgcc gagcggtcga tctcgcagta ggtgccgtcg acggcgatgc cgggatagcg
     1921 gaacgggtcg ccgcgattgg tcgtgcgcag gatggtgccg cccaggtggg caatgccgcg
     1981 cacggattcg cgcgtcagcg gcaccacttc gtcttcgcct aacagaccgg cgtagccccg
     2041 cttgacgccc agcacttccc atccgcgtcc gatcgcggac agcaccgcgg cgcggatgac
     2101 ggcgttcaag ccgggtgcgt caccgccgcc ggtcgaaatc gcgatgcgca tccgccctcg
     2161 cgtctaacga tcgccgcgcg gcgcgcccat cagccggcac gctcggcacg cgcacgctcg
     2221 atcaagccga gcacctcgcc ctccttggcg tgacgcccga gcttcgattt ttcgaagatc
     2281 ggcctcccat cgagcgacac ctcgaaccgt cctccgcgcg acggctgcat cgtgatcgac
     2341 gcatccgggt accgtgcctc gagctcggcc gccaacctgg tggccctcgg ctcgtagttt
     2401 caaagcgtac agtattcgat cgtaatcgtc atgcgccctc acaggtagcc accatcggcg
     2461 cgcgctccgc gccacagcga accatacgcc tccaacgcag agcaatgcca gcggcagccg
     2521 agtaccaagc tcgccgagcg atccgtggag tctccgccgt tatccgtttg atccgtcttg
     2581 a
//
LOCUS       DASUCJ010000311         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_141198, whole genome shotgun sequence.
ACCESSION   DASUCJ010000311 DASUCJ010000000
VERSION     DASUCJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141198"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU90_03735"
     CDS             complement(<1..306)
                     /locus_tag="VFU90_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478914.1"
                     /translation="MTVSVSAREVLASRLALRPLPFVRFATDVLGIVLSKAWRVLLLV
                     AVDGIEPAELDGEERETARKLFGAVDTIPPAARRTLVWRLGRGSGKTTLAAALGLWIA
                     "
     gene            complement(318..653)
                     /locus_tag="VFU90_03740"
     CDS             complement(318..653)
                     /locus_tag="VFU90_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478915.1"
                     /translation="MNKEQHELSNRQQAIGAQKANEQENESHPGPLTSVSFSRPVVCG
                     PKSVSMIEPGKPGSAGWRVDAMLSAQLVRVSLEVERSDKSRIRFTTLVPLGDCHLRYE
                     REFAAPPTE"
     gene            complement(650..967)
                     /locus_tag="VFU90_03745"
     CDS             complement(650..967)
                     /locus_tag="VFU90_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478916.1"
                     /translation="MLFEKHGALVGRLLFLGRALIAHEPGDLQHNYGFVAFDAKPGVV
                     HWIYVKGVYRRMGVGRALWKAAGFGKPVAATHATDMAFGMMGAAVARYGVTYDPYLLE
                     DIR"
     gene            complement(1269..1616)
                     /locus_tag="VFU90_03750"
     CDS             complement(1269..1616)
                     /locus_tag="VFU90_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478917.1"
                     /translation="MSRVERWDAFDVSTLTQGDFDRMTLESLSYLRAIVRSVPTGRQM
                     GAGIAAAKHVLDFVAWRAGHDLAERKFALAAREGSDEKTGRAAQLDRLDDEELELLAK
                     FAEEREKKAKVPA"
     gene            complement(1682..>2048)
                     /locus_tag="VFU90_03755"
     CDS             complement(1682..>2048)
                     /locus_tag="VFU90_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478918.1"
                     /translation="VMGWVLMAALIAASFFETDCGGRVDEPEDVAAESTADPGETVVD
                     CSAMLDGYPLDFLVTIRGPSASLQLTWAGPDATSAVRTYPDWDGAIAVETASTTLEWQ
                     VGWTQLKDTNGLVSSSSCR"
BASE COUNT          343 a          752 c          601 g          352 t
ORIGIN      
        1 ggcgatccag aggcccagcg ccgccgccag cgtcgtcttc cccgagcctc gccctaggcg
       61 ccacaccagc gtccttcgcg ccgccggggg gatggtgtcg acggcgccaa acagcttgcg
      121 cgccgtctct cgctcctctc cgtcgagctc cgccggctcg atgccgtcga cggcgacgag
      181 cagcaggacg cgccacgcct tcgagagcac gatgccgaga acgtccgttg caaagcgaac
      241 aaatggcagc gggcgcagcg cgagccgcga ggcgaggact tcgcgggcgg agacggagac
      301 ggtcatcgct cgctgggtca ttccgtcgga ggcgccgcga actcccgctc ataccgaagg
      361 tggcaatcgc cgagcggtac gagcgttgtg aaccgaatgc gcgacttgtc cgaccgttcg
      421 acttcgaggc tcacgcgaac gagctgcgcg ctcagcatcg cgtcgacgcg ccagcctgca
      481 cttcccggct tgccgggctc gatcatcgag acggatttcg gcccgcagac gacggggcgt
      541 gagaatgaga cgctcgtgag cggccccgga tgcgactcat tctcttgctc gttcgccttc
      601 tgagcaccga tcgcctgctg ccgattgctg agttcgtgct gttccttgtt catcgaatgt
      661 cctccaacaa atacgggtcg tacgtcactc cgtagcgcgc caccgcggcg cccatcattc
      721 caaaggccat gtccgtcgcg tgtgtcgcgg ccaccggctt cccgaatccc gccgccttcc
      781 aaagcgcgcg gcccacaccc atccgccggt acacgccctt cacgtagatc cagtggacca
      841 cgcctggctt cgcatcgaac gccacgaacc cgtagttgtg ctgcaagtcc ccaggctcgt
      901 gcgcgatgag cgcgcgccca agaaacaaga gccgcccgac gagggcccca tgcttctcga
      961 agagcacgtc gtcgcgaacg aaccgaaccg aacgctcgcc tctcagatgg cgcagccacg
     1021 cggatcggat aaacgcgata tcgtcgttcg tagccgaacg aatggcaacc gggagcatca
     1081 cttttgcgag ctcccgatat ggtactggcc gcaccggtcg caccggtacg cgttcagttt
     1141 ttcgcgccag tccgcgttcg ccttccgatt ccggagcggg ccgcgccgat gagtctgcgc
     1201 ctcgacggcc agcatcgcgg cgccgcgcga cggaaacacc tgcttaccct cgcatgcctc
     1261 tatgcctctc atgccggcac ctttgctttc ttctctcgct cctcggcgaa tttcgccaag
     1321 agctccagct cttcgtcgtc cagtcgatca agctgcgccg cgcggcccgt cttctcgtcc
     1381 gacccttcgc gcgccgcgag cgcgaacttc cgttccgcca ggtcgtgccc cgcgcgccat
     1441 gcgacgaagt cgaggacgtg cttcgccgcc gcgatgccgg cgcccatctg ccggcccgtc
     1501 ggcacgcttc gcacgatggc ccgcaagtac gagagcgact cgagcgtcat gcgatcgaag
     1561 tcgccctgcg taagcgtcga cacgtcgaac gcgtcccacc gctcgacgcg cgacacgtcg
     1621 atagccgccc cctggccccc tgagtttttt cggtcggacg ggtcggacat agtttgtcac
     1681 gtcatctgca gctcgacgag gacacaaggc cattcgtgtc cttcaactgc gtccacccta
     1741 cttgccactc gagcgtcgtc gacgccgtct cgaccgcgat ggccccgtcc caatccggat
     1801 acgtccggac agcgctcgtc gcatcggggc ccgcccacgt gagttgcagc gacgcgctcg
     1861 gcccgcgaat cgtcaccagg aagtcaagcg ggtacccgtc gagcatcgcc gagcagtcga
     1921 ccaccgtttc cccggggtcc gccgtcgact ccgcagcgac gtcttccggc tcgtcgacgc
     1981 gacctccgca gtccgtttcg aagaacgaag cggcaataag cgccgccatc aggacccatc
     2041 ccataact
//
LOCUS       DASUCJ010000312         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_431317, whole genome shotgun sequence.
ACCESSION   DASUCJ010000312 DASUCJ010000000
VERSION     DASUCJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431317"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFU90_03760"
     CDS             <1..277
                     /locus_tag="VFU90_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478919.1"
                     /translation="IALVAFDHNRYAFPVMVAYVAYGLLKTFVLGLVDRLPSADPLLD
                     DDEDDEAMERSVELAEHPHRRRRRRRRNVPLGEQIHPFNPGPDEGRE"
     gene            295..534
                     /gene="purS"
                     /locus_tag="VFU90_03765"
     CDS             295..534
                     /gene="purS"
                     /locus_tag="VFU90_03765"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5478920.1"
                     /translation="MHIVPRKGILDPQGKAVADALHTLGFGDVQDARIGRHVVLDVAA
                     TTPDAAGDSVRKMCDRLLANPVTEDYEIARVEGLP"
     gene            531..>1126
                     /gene="purQ"
                     /locus_tag="VFU90_03770"
     CDS             531..>1126
                     /gene="purQ"
                     /locus_tag="VFU90_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5478921.1"
                     /translation="MKFGVVTFPGSNCDYDAFRTVVDVLGEHAVYLWHKEHDLQNADV
                     VILPGGFAYGDYLRAGAIARFSPIMQEVAVHARRGGPVLAVCNGFQIACEAGLLPGAL
                     LRNASLTFLGQPVRVRVENSDTMFTNRYAHGQVLTMPIAHGEGRYTAEADTLDRLEGE
                     GRVVFRYVNARGGLDDESNPNGSMRAIAGIVSAEGNVLG"
BASE COUNT          189 a          368 c          388 g          181 t
ORIGIN      
        1 catcgcgctc gtggcgttcg accacaaccg ctacgcgttc ccggtgatgg tcgcgtacgt
       61 ggcgtacggg ttgctcaaaa cgttcgtgtt aggactggtc gatcggttgc cgagcgccga
      121 cccgctgctc gacgacgacg aggacgacga ggcgatggag cgctcggtcg agctcgccga
      181 gcatccgcac cgacgacggc gccgccggcg tcgcaacgtt ccgttaggcg aacaaatcca
      241 cccgttcaac cccgggccag acgagggccg tgaatgagcc ggtatagagt gtccgtgcac
      301 atcgtgccgc ggaaggggat tctcgatcca cagggcaagg ccgtcgccga cgcgctgcac
      361 acgctgggct ttggcgacgt gcaggacgcg cgcattggcc gtcacgtcgt gctcgacgtg
      421 gccgcgacca cgccggacgc cgccggcgac tcggtgcgca agatgtgcga ccggctgctc
      481 gccaatcccg tcaccgaaga ttacgaaatc gcgcgcgtcg aggggctgcc atgaagttcg
      541 gtgtcgtcac gtttccggga tccaactgcg actacgatgc cttccgcacc gtggtcgacg
      601 tgttaggcga gcacgcggtg tatctctggc acaaagagca cgacctgcag aacgccgacg
      661 tcgtgatcct gccgggcggg ttcgcctacg gcgactacct gcgcgccggc gcgatcgcgc
      721 ggttcagccc gatcatgcag gaggtcgcgg tgcacgcgcg gcgcggcgga cccgtactcg
      781 cggtgtgcaa cggattccag atcgcgtgtg aggccgggtt gttgccgggc gcgctgctcc
      841 gcaacgcgag cttgacgttc ctgggccagc ccgtccgcgt ccgggtcgaa aactccgaca
      901 cgatgttcac caaccgctac gcgcacggac aggtgctgac catgccgatc gcgcacggcg
      961 agggccgcta cacggccgag gccgacaccc tggatcggct cgagggcgag gggcgtgtcg
     1021 tgttccggta cgtcaatgcg cgcggcggtc tcgacgacga atcgaatccc aatggatcga
     1081 tgcgcgccat cgccggcatc gtgagcgccg aaggcaacgt gttggg
//
LOCUS       DASUCJ010000313         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_538127, whole genome shotgun sequence.
ACCESSION   DASUCJ010000313 DASUCJ010000000
VERSION     DASUCJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538127"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1454)
                     /gene="metG"
                     /locus_tag="VFU90_03775"
     CDS             complement(<1..>1454)
                     /gene="metG"
                     /locus_tag="VFU90_03775"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5478922.1"
                     /translation="GITPEALIEGVWKRHTADLADFGIAYDHYSSTHTQANRELTARI
                     WKALKAGGHTESRPVKQFYDPVKAMFLPDRYIKGECPKCHSKDQYGDGCEVCGTAYTP
                     ADLINPYSVVSGATPELKESTHFFVKLADFEAMLKGWLAQRRDAGGLQAEVVNKLQEW
                     FADGLRSWDVSRDAPYFGFEIPDQPGKYFYVWVDAPIGYLSSFRDYCTAWNAKHPEKP
                     IRFEDFVERDDAAKTGTEMVHFIGKDIIYFHTLFWPAMLHGAKLRTPTAVNVHGFMTV
                     DGAKMSKSRGTFINARTYLDHLDPDYLRYYLATLLGPTLADLDLDLKAFEERVNSHVV
                     GKWVNIASRTAGFVQKFFGGKLVDALPESEGARYASAVERLRACAALFEARDYAAVTR
                     LIVEVADEANGYVAEKAPWVLAKDESRRAELHVVCTLAINYFRLLATYLAPIVPTMAR
                     LVGENFLGMMATGFRDADTLLLGHEIRPFATRIDR"
BASE COUNT          269 a          483 c          437 g          265 t
ORIGIN      
        1 cggtcgattc gcgtcgcgaa cggccggatc tcgtgtccaa gcagcagcgt atcggcgtcg
       61 cggaaacccg tcgccatcat gccgagaaag ttctcgccga cgagtcgcgc catcgtcggc
      121 acaatcggcg cgagataggt cgccagcaac cggaaatagt tgatcgcgag cgtgcagacg
      181 acgtgcagct ccgcgcgcct gctctcgtcc ttcgcgagca cccacggcgc cttctcggcg
      241 acatagccgt tcgcctcatc ggcgacttcg acgatcaacc gcgtcacggc cgcgtagtcg
      301 cgtgcttcga ataacgcagc gcacgcgcgc aagcgttcga ctgccgacgc gtagcgcgcg
      361 ccctcggact caggcaaggc gtccacaagc ttcccgccga agaacttctg cacgaaaccc
      421 gccgtccggc tcgcgatgtt cacccacttg ccgaccacgt gcgaattgac ccgctcctcg
      481 aacgccttca gatcaagatc aagatccgcc agcgtcggcc cgagcaatgt cgcgaggtaa
      541 taccgcaaat agtccggatc gagatgatcg agatacgtcc gcgcattgat gaacgtgccg
      601 cgcgacttcg acatcttggc gccgtcgacg gtcatgaagc cgtgcacgtt caccgccgtc
      661 ggcgtgcgga gcttcgcgcc gtgcagcatc gccggccaga acagcgtgtg gaaatagatg
      721 atgtccttgc cgatgaaatg caccatctcg gtgccggttt tggcggcatc gtcgcgctcg
      781 acgaaatcct cgaagcggat gggcttctcc ggatgcttcg cgttccacgc cgtgcagtag
      841 tcgcggaacg acgacagata cccgatcggc gcatcgaccc agacgtagaa atacttgccc
      901 ggctgatccg gaatctcgaa gccgaaatac ggcgcgtcgc gcgacacgtc ccagctgcga
      961 aggccatcgg cgaaccattc ctgcagcttg ttgacgactt ccgcctgcaa gccgccggcg
     1021 tcgcggcgct gtgccagcca gcctttcagc atcgcctcga agtcggcgag cttgacgaag
     1081 aaatgcgtcg attccttcaa ttccggcgtt gcgcccgata ccaccgaata gggattgatc
     1141 aggtcggccg gcgtgtaggc ggtgccgcag acctcgcagc cgtcgccata ctgatccttg
     1201 ctgtggcact tcgggcattc gcccttgatg tagcggtcgg gcagaaacat cgccttgacc
     1261 gggtcgtaga actgcttcac cggccgcgac tcggtgtggc cgccagcctt gagcgccttc
     1321 cagatgcgcg cggtcagctc gcggttcgcc tgcgtgtgcg tggacgagta atgatcgtag
     1381 gcgattccga aatccgcgag gtccgcggtg tgccgcttcc acacgccctc gatcagcgcc
     1441 tcgggcgtga tgcc
//
LOCUS       DASUCJ010000314         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_981390, whole genome shotgun sequence.
ACCESSION   DASUCJ010000314 DASUCJ010000000
VERSION     DASUCJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981390"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFU90_03780"
     CDS             <1..336
                     /locus_tag="VFU90_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU5478923.1"
                     /translation="KSTRLETRCPGADINAYLAVAACLAAGLDGIERKLKLTTAPITG
                     TNAGAESIPRAPRTLIETTRIFRASERARDWFGDEFVDHFAATREWEWRQWQDAVTDW
                     ERSRYFEII"
     gene            complement(341..1126)
                     /locus_tag="VFU90_03785"
     CDS             complement(341..1126)
                     /locus_tag="VFU90_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478924.1"
                     /translation="MRSLFKHLLRAGMVAALGICASAASAAPAAPAVTTCAGGDVAPG
                     VYGRVVVTGTCTVTGDVTVDGNVTIADGGDLDAAYAGTQLTINGDISVGKGAKLGLGC
                     TFGYHDCGFSPPNWIGKVTVNGNIVANQALTMYLDFTTVHGNIVVNGGGDLSLVDHPP
                     IEDGLVLPIKDNHVDGNIVVHGWQGAWFGIIRNSVGGNVMASHTEGTRLGDGDTPDST
                     EIATNVIGGNLICVQNMPSAQIGDSHGSPNTVGGNKIGECAGL"
BASE COUNT          238 a          496 c          442 g          255 t
ORIGIN      
        1 aaatcgacgc gcctcgaaac gcgctgcccg ggcgctgaca tcaatgccta tctcgcggtt
       61 gccgcctgcc tcgccgcggg cctcgacggc atcgagcgga agctgaagct cactaccgcg
      121 ccgatcaccg gcaccaacgc cggcgccgag agcatcccac gcgcgccgcg tacgctgatc
      181 gagaccacgc gcatcttccg cgcatccgag cgcgcgcgcg actggtttgg cgacgaattc
      241 gtcgaccact tcgccgccac tcgcgaatgg gagtggcggc agtggcagga cgcggtgacc
      301 gactgggagc gcagccgcta tttcgagatc atctgaggcg tcagagcccc gcgcactcgc
      361 cgatcttgtt gccgcccacg gtattcggcg agccatggct gtcgccgatc tgcgcggatg
      421 gcatgttctg cacgcagatc aggttgccgc cgatgacgtt ggtcgcgatc tcggtcgagt
      481 ccggcgtgtc cccgtcaccg agccgcgtgc cctcggtgtg cgacgccatc acgttgcccc
      541 ccacgctgtt gcggatgatg ccgaaccagg caccctgcca gccatgaacg acgatgttgc
      601 cgtcgacgtg attgtccttg atcgggagca cgagaccgtc ctcgatcggc ggatgatcga
      661 ccagcgacag atcgccgccg ccgttcacga cgatgttgcc atgcacggtc gtgaagtcga
      721 ggtacatcgt cagcgcctga ttggcgacga tgtttccgtt gacggtcacc ttgccaatcc
      781 agttcggtgg cgaaaacccg cagtcgtgat aaccgaacgt acagccaagc ccgagcttcg
      841 cacccttgcc gacgctgatg tcgccgttaa tcgtcagctg tgtgccagcg taggccgcgt
      901 cgaggtcgcc gccgtcggcg atggtgacgt tgccatcgac ggtcacgtcg cccgtgaccg
      961 tgcaggtgcc ggtgacgacg acgcgcccgt agaccccagg agcaacgtca ccgcccgcgc
     1021 aggtcgtcac ggcaggtgcg gcgggagcgg cggacgctgc cgatgcacag attccgagtg
     1081 cggcaaccat gcctgcgcga agcagatgct tgaacagact tctcatcgtc taatcctcct
     1141 ggagactaca atccgatacg ttcgacgtga gcgcgtcgat cgacgccgga tgtggacgcg
     1201 ctgtggagcg cgcccccccc gacggcatcg cttgattgtc gttcgcgggc gccggcgtgt
     1261 cgagcgaata ttcgcgccca cgctctgacg ggccgcgcgc ggtagcatca cccgatctcc
     1321 ccgcacttcg acttcccgtg tcctccattc acacggtctc gttccccgct ccgatccact
     1381 tcggcccagg cacccgcaag ttgatcgctt cttacctgcg cgagcagcgc g
//
LOCUS       DASUCJ010000315         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1073476, whole genome shotgun sequence.
ACCESSION   DASUCJ010000315 DASUCJ010000000
VERSION     DASUCJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073476"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFU90_03790"
     CDS             <1..356
                     /locus_tag="VFU90_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478925.1"
                     /translation="TVGRVYDSLPPSDRAKAVAFAQNYGEAAAVEFFAPRVPVISGHN
                     QYWLWGPRGHDGSVVIDIAGDCGAKQHLFKSTSKAATFSAPYVMPYEDDIPISVCRGL
                     TVPLQTLWPTLKHYE"
     gene            605..>1061
                     /locus_tag="VFU90_03795"
     CDS             605..>1061
                     /locus_tag="VFU90_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478926.1"
                     /translation="MAIVPALPPHQVQIISHFDYLSVDAQRRRVYAAHSGSQALLVVN
                     ANTGAVLGQVETGAVHGNAVDEATGHVYTGDGQSGTVSEVDPVKMTVVNQVDVGHPID
                     ALAYDPKTGRIFADEDSGTQVFVVDARSFKLLGSVPIPGHDLEYLAVDPD"
BASE COUNT          204 a          338 c          327 g          192 t
ORIGIN      
        1 cgaccgtcgg gcgtgtctat gattcgctcc caccgtccga tcgggcgaag gcggtcgcgt
       61 tcgcgcagaa ctacggcgag gctgctgcgg tcgagttctt cgcgccgcgc gttcccgtaa
      121 tcagcgggca caaccaatac tggctatggg ggccgcgggg ccatgacggc agcgtcgtga
      181 tcgacatcgc cggcgactgc ggcgcgaagc agcatctctt caagagcacg tctaaggcgg
      241 cgacgttttc ggcgccgtac gtcatgccgt acgaggacga catcccgata tcggtatgcc
      301 gggggctcac ggtgcctttg cagacgttgt ggccgacgct caagcattac gaataggcag
      361 ccgagcgtcg ctcggcatac gatgctgctc gcagaaaggc cgcgccggtc gaccgtaaag
      421 gtcgaccgct acatttccgt cgcgcgaggc cgctacattg tcgtcatgcg aggtcgaccg
      481 ctacatctct ttatgacaag gttaagaagc attcagatac cttaatgcag ccgcacaggc
      541 acgatggcga tcgtcgtcgg aatcgcatac ggtttgcgtc tacggcgagg ggagacacac
      601 gcggatggct atcgttcctg cattaccgcc gcatcaagtc cagatcatca gccacttcga
      661 ctacttgtcc gtcgatgcgc agcgcaggcg cgtctacgcg gcgcacagcg gcagccaagc
      721 tctgctcgtc gtgaacgcaa acaccggcgc cgttctcggt caggtcgaga ccggcgccgt
      781 ccacggcaac gccgtcgacg aagcgaccgg gcacgtatac acgggcgacg gccagtcggg
      841 caccgtatcg gaagtcgacc cggtcaagat gaccgtcgtc aatcaagtcg acgtcggtca
      901 tccgatcgat gcgctcgcct acgacccgaa gaccggacgt atcttcgcgg acgaggacag
      961 cggcacgcag gtattcgtcg tcgacgctcg ttcgttcaag ctgctcggca gcgtgccgat
     1021 cccgggtcac gatctcgaat acctcgccgt cgatcccgat c
//
LOCUS       DASUCJ010000316         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_996874, whole genome shotgun sequence.
ACCESSION   DASUCJ010000316 DASUCJ010000000
VERSION     DASUCJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996874"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFU90_03800"
     CDS             <1..831
                     /locus_tag="VFU90_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478927.1"
                     /translation="PDSTWLVQDSIARACLDTLSDGSVRPVTVYQVAAASDTSPAVLD
                     QVALISEHVGERVRAALGGGTGALPNVDSLVPWRRLAGRVPIRIVLHRDAAATWAVDG
                     SAGPTKAKLATLYAAVLRSMPDDALDVVLPAPPAPDSVLVWVTLTSSESSIRPLPRPG
                     YPMVAVFRMSGVAFTPAIARRNNPVPAYPLDAAERRIGADVVVGLVVRPGGRADAEVR
                     TIVISSPEPMDARAHDHFAREFRSAVEGVVKEMRFEPARIGGCVVPQRADFPFSFRRG
                     "
     gene            997..>1582
                     /locus_tag="VFU90_03805"
     CDS             997..>1582
                     /locus_tag="VFU90_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478928.1"
                     /translation="MRLQRWAGAFAAALGMTATARAQPPAAHDSSNVGVATPCRNPVE
                     RWAGAFDFVSQSLCAVDSANISASELLRLDQRIGEAAREALGGSDALIPRADTLGALG
                     DDGAVPLTIVLHRDAPATWHPAPSTDSADARLATFYRVVLGSMAPNDQRTPWPTGATS
                     DSAIIRVWMSTQQFGRLMNAGIAWRVVMEATIDAD"
BASE COUNT          234 a          538 c          543 g          267 t
ORIGIN      
        1 cccgactcga cctggctcgt ccaggattcc atcgcccggg cgtgtctcga tacgctgtcc
       61 gacggaagcg tgcgcccggt caccgtgtac caggtcgcgg ccgcttccga cacgagcccc
      121 gctgtgctcg atcaggtcgc gctgatttcc gagcacgtcg gcgagcgcgt gcgtgctgcg
      181 ttaggcggcg gtacgggtgc gctgccgaac gtggattcgc tggtgccgtg gcgacgcctc
      241 gccggacgcg tgccgatccg gatcgtcctc caccgcgatg ccgcggcgac ctgggcggtc
      301 gacggcagcg ccggcccgac gaaagcgaag cttgcgacgc tttacgccgc cgtgctccga
      361 agcatgcctg acgatgcgtt ggatgtggta ttgcccgcgc cacccgcgcc cgactcggtg
      421 ctggtctggg tcacgctgac cagcagcgaa tcgtcgattc ggccgcttcc gcggccaggc
      481 tatccgatgg tggcggtctt tcgcatgtcc ggcgttgcgt tcacacccgc catcgcgagg
      541 cggaacaacc cggtgcctgc ctaccccctg gatgccgcag agcggcggat cggcgcggac
      601 gtcgttgtcg gcctcgtcgt taggccggga ggccgcgctg acgcggaggt gaggaccatc
      661 gtcatctcat cgccggagcc gatggatgcg agagcccatg accactttgc tcgtgagttc
      721 aggtcggccg tcgaaggcgt cgtcaaggag atgcggttcg agcccgcccg catcggcggc
      781 tgcgtcgtgc ctcagcgggc ggactttccc ttcagcttcc ggcgcgggta agtcgctgcc
      841 cggcagcatg gccaagcagt ccatcagaca tgcgcctcga cgtcatgagc gacaatccat
      901 tcgagacggc gccgtcgagg tgcgcgaccg tgtttcacga ttccgtcgtt cgtcattcgg
      961 gaaagccggt gccacatgcg ttggaggatg agtcctgtgc gactgcagcg ttgggccggg
     1021 gcgttcgccg ccgctctcgg gatgacggcc accgcgcggg cgcagccccc agccgcgcac
     1081 gattcgtcga acgtcggcgt ggcgaccccg tgccgaaacc ccgtcgaacg ttgggccggg
     1141 gcgttcgatt tcgtcagtca atcgctctgc gccgtcgaca gcgccaatat ctcggcaagc
     1201 gagctgctcc ggctcgacca gcgcatcggc gaggcggcgc gcgaagcgtt aggcgggtcg
     1261 gacgcactca tcccgcgcgc cgatacgtta ggcgctctcg gtgacgatgg cgcggtgccg
     1321 ctgacgatcg tcctgcaccg cgacgcgccg gccacctggc acccggcgcc gtccacggac
     1381 agcgcggatg cacgcctggc cacgttctac cgcgtggtgc tcggcagcat ggcgcctaac
     1441 gaccagcgga ctccctggcc gacaggagcg acgagcgact cggcaataat ccgcgtgtgg
     1501 atgagcacgc agcaattcgg ccgactcatg aatgccggga ttgcatggcg cgtcgtcatg
     1561 gaagcgacca tcgacgcgga cg
//
LOCUS       DASUCJ010000317         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_920548, whole genome shotgun sequence.
ACCESSION   DASUCJ010000317 DASUCJ010000000
VERSION     DASUCJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920548"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1122
                     /locus_tag="VFU90_03810"
     CDS             130..1122
                     /locus_tag="VFU90_03810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEU5478929.1"
                     /translation="MSTDIALHGVTENGARNSAEAEFDVFVVRKEKIADDAMSITLRS
                     AGSPLPEWSPGAHIDLVLPSKKVRQYSLCGDPSNREEWRLGVLREQAGRGGSAYVCDE
                     LAEGTRLTVRGPRNHFALVPAASYLFIAGGIGITPILPMLRAATQAGARLELWYGGRR
                     ASSMAFVEELGEYGDRVHLWPQDERGLLPLDELLAGRGDDTLVYCCGPAPLLEAAEAR
                     SREWPTGTLHVERFAPIAVDHAGDQQFEVELARSRKTLTVLPGTSILHTIEAAGVFVL
                     SSCQEGTCGTCETDVLEGTPDHRDCVLTEAERAAGDIMMICVSRSRSSRLVLDL"
     gene            1196..>1832
                     /locus_tag="VFU90_03815"
     CDS             1196..>1832
                     /locus_tag="VFU90_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU5478930.1"
                     /translation="MRTSSARIFQLLDAFNSAAKGLSLTEISRRSGLPLTTVHRFAHE
                     LRRLGVLECDPDGRYRIGLRLWEIAAHAPRGVGLREAAMPFLEDLYEATHENVQLAVR
                     DAHDVVYIEHIAGRRAVGVRTQVGLRFPLHATGVGLVLLAFADEALQEEVMNAPLAGF
                     TPKTIQDPAMLRRKLADVRQQGFAISDRQVTMDALSVAAPIRDAEDAVVAAV"
BASE COUNT          317 a          592 c          605 g          318 t
ORIGIN      
        1 gcgcccagct ggagtcggaa gaccccatcg agttgtggca gtggctcacc ggccatccgg
       61 tgggccacgg acaatccgat tcgctgaggc agccggcgcc gcacgacctc gtcgcgatgg
      121 ggagttgaga tgtcgaccga catcgcgctt catggcgtga cggagaacgg cgcgcgcaac
      181 agcgcagaag ccgagttcga cgttttcgtc gtccgaaaag aaaaaatcgc ggacgacgcg
      241 atgtcgatca ccctgcgcag cgccggatcg ccgctacccg agtggtctcc aggcgcgcac
      301 atcgatctcg tgctgccaag taaaaaggtg cggcagtact cgttgtgtgg cgacccctcg
      361 aaccgcgagg aatggcgtct tggcgtgctg cgcgagcagg caggccgcgg cggttcggcg
      421 tacgtgtgcg atgagctcgc cgagggaacc cggctcacgg tgcgtgggcc acgaaaccat
      481 ttcgcccttg tgccagcggc aagctacctg ttcatcgccg gtggtatcgg catcacgccg
      541 atcctgccaa tgctgcgcgc cgcgacacag gccggcgcgc gattggagct ttggtacggc
      601 ggccgccgag cgtcgtccat ggcctttgtc gaagagttgg gcgagtacgg cgatcgcgtt
      661 catctgtggc cccaagacga acgcggtctg ctgccgctcg acgagttgct cgccggccgc
      721 ggcgacgaca ccctggtcta ctgctgcggc ccagcgccat tgctcgaagc cgccgaggcg
      781 cgctcccgcg aatggccgac cggcacgttg catgtcgaac ggttcgctcc gatcgctgtc
      841 gatcacgcgg gagaccagca gttcgaggtc gaactcgccc gttctcgcaa gacgttgacc
      901 gtgctgcccg ggacgtcgat cctgcatacc atcgaggcgg caggagtctt cgtgctctcg
      961 tcctgccagg agggcacctg cggcacctgc gagacggacg ttctcgaagg gacgccggat
     1021 caccgtgatt gtgttctcac cgaagccgag cgtgccgcgg gcgacatcat gatgatctgc
     1081 gtttcccggt cacgaagcag ccgactcgtg ctcgacttgt agtagccgcc gtcacccgtg
     1141 gcggctagga ggcgacatca tgctggcggc caagcctgaa cgggctagcg atggcatgcg
     1201 cacgtcgtcc gcgcggattt ttcaactcct cgacgcgttc aactcggcgg caaaagggct
     1261 gtcgctcacc gaaatcagcc ggcgcagcgg actgccgctc accacggtgc accgcttcgc
     1321 gcacgagttg cgcaggctgg gagtgctcga gtgcgatccc gacggcaggt accggattgg
     1381 cttacgcctg tgggagatcg ccgctcacgc cccgcgcggc gtcggattgc gcgaagcggc
     1441 gatgccgttc ctcgaagacc tctacgaagc cacccacgag aacgtgcagc tcgccgttcg
     1501 cgacgctcac gacgtcgtct acatcgagca catagccgga cgacgcgcgg tcggcgtgcg
     1561 cacccaggtc ggattgcggt ttccgttgca cgccaccggc gttgggcttg tgttgctcgc
     1621 gttcgccgac gaggcattgc aggaagaagt catgaacgcg ccattggctg gcttcacacc
     1681 gaagacgatt caagatcccg cgatgttgcg ccgcaaactc gccgacgtcc gacaacaggg
     1741 tttcgcgatc agcgatcgtc aggtgacgat ggacgccctc tcggtggccg cgccgatcag
     1801 ggacgccgaa gacgctgtgg tcgccgcagt tt
//
LOCUS       DASUCJ010000318         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_614815, whole genome shotgun sequence.
ACCESSION   DASUCJ010000318 DASUCJ010000000
VERSION     DASUCJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614815"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFU90_03820"
     CDS             <1..201
                     /locus_tag="VFU90_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478931.1"
                     /translation="TEVCSRALRALDDRGVFGAPREVVLVSAMHDESEESFMSAISEL
                     NSERATARVSAEREAGFAIVPR"
     gene            complement(232..660)
                     /locus_tag="VFU90_03825"
     CDS             complement(232..660)
                     /locus_tag="VFU90_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478932.1"
                     /translation="MKSIGSTIHGVTVAMGILGVCFVARDARADLHGMRLVLNFGGTQ
                     YTGAAQTVEESATAVNGAVQYRVNLHATASDGRKLLCAIAVPSHADAESYRTELLNEH
                     TAVVTCACSHPVTRDGTGDTFVFLNTMTPSAGDSLSIESR"
     gene            complement(789..1280)
                     /locus_tag="VFU90_03830"
     CDS             complement(789..1280)
                     /locus_tag="VFU90_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478933.1"
                     /translation="MPNGNQPTNPPGTMNGNQPANPSGTMNGNQPANPSGAMNGNQPA
                     NPFGTTNGNQPANPSGTMNGSQPANPFGATTGNQPTNPHATPTNPYATATNPFATQFN
                     PYATPANPYATQTNPFATQYNPFATATNPYATPTDPNFGLPSSFGAAPGYGNPYGFGH
                     PPY"
     gene            1267..>1785
                     /locus_tag="VFU90_03835"
     CDS             1267..>1785
                     /locus_tag="VFU90_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478934.1"
                     /translation="MPFGIAVSLGAGAPFVPGCALGAPFVMSVVGSPAGIMLGAAVEL
                     GAAVDDEDGALVSVPVLEGAGWLVDAGAPELPELEEAGAGALVCTSPPDPVVVGAEPH
                     ATRACIVATAETMYINVDRMAGLLDEPASRSYVVPVAPCAASLPRNRRRRAGERPPLA
                     HARNARVTGRGRV"
BASE COUNT          228 a          569 c          661 g          327 t
ORIGIN      
        1 accgaggtct gctcccgcgc gctgcgagct ttggacgatc gaggcgtgtt cggtgcaccg
       61 cgagaggtcg tgctcgtatc cgcgatgcac gacgagtcgg aggagagctt catgagcgcg
      121 atttcggagc tcaattcgga acgagcgacc gcgcgcgtgt cagccgagcg ggaggctggt
      181 tttgcgatcg tgccgcgcta gtcgagcctc cgggctcttg cgaccgtccg gctagcgact
      241 ctcgatgctg agcgaatcgc cggcgctggg cgtcatcgtg ttgaggaaca cgaacgtgtc
      301 ccccgttccg tccctggtca ccgggtggga gcaggcgcac gtcaccacgg ccgtgtgctc
      361 gttcaggagc tcagtgcggt agctctcggc gtctgcgtgg gatggaaccg cgatcgcgca
      421 cagcagcttc ctgccgtccg aagccgtcgc atgcaggttg acccggtact ggactgcgcc
      481 gttcacggcg gtcgcgctct cttccacggt ctgggcggcg cccgtgtatt gcgtcccgcc
      541 gaagttgagg acgagtcgca tcccgtgcag gtctgcccgc gcatcgcgcg ccacgaagca
      601 gacaccgaga atgcccatcg ccaccgtcac cccatggatc gtggatccga tgctcttcat
      661 gcggctcttt cctcccagga acgagcctcc atcatcgcac gggaatcgac cgctgccgca
      721 gatgccggaa aagccgaccg gcggaaccgc cgattgctcg gcgattccgc tcggccgggg
      781 cgactgcctc agtagggcgg gtgtccgaag ccgtacggat ttccgtagcc gggcgcagcc
      841 ccgaagctgg agggcaggcc gaagttgggg tccgtcggcg tggcgtaggg attcgtcgcc
      901 gtggcgaacg ggttgtactg cgtggcgaac ggattcgtct gcgtggcgta ggggttcgcc
      961 ggcgtggcgt aggggttgaa ctgcgtggcg aacggattcg tcgccgtggc gtaggggttc
     1021 gtcggcgtgg cgtggggatt cgtcggctgg ttgccggtcg tcgcgccgaa cggattcgcc
     1081 ggttggctgc cgttcatcgt gccggatgga ttcgccggct ggttgccgtt cgtcgtgccg
     1141 aacggattcg ccggctggtt gccgttcatc gcgccggacg gattcgccgg ctggttgccg
     1201 ttcatcgtgc cggacggatt cgccggctga ttgccgttca tcgtaccggg tggattcgtc
     1261 ggctggttgc cgttcggcat cgcggtgtca ctcggcgccg gcgcgccgtt cgtccccggc
     1321 tgcgcgctcg gcgcgccgtt cgtcatgtcg gtcgtgggct ccccggcggg catcatgctc
     1381 ggtgccgccg tggagctggg tgcagcggtg gacgacgagg acggagcgct cgtatcggtg
     1441 ccggtgctcg aaggcgcagg gtggctcgtc gacgcgggcg cgcccgagct ccccgagctc
     1501 gaggaggccg gcgccggcgc gctggtctgc acctcgcccc cggatccggt cgtggtgggg
     1561 gcagagccgc acgcgacgag ggcgtgcatc gtggcgacgg cggagacgat gtacatcaac
     1621 gtggatcgca tggcagggct ccttgacgag ccggcgagcc gttcatacgt cgtgccggtg
     1681 gccccatgcg cagcttctct gccgcggaac cgacggcgcc gcgcgggcga acggcctccc
     1741 ctcgctcatg cgaggaacgc ccgcgtgacc ggccgcggcc gcgtg
//
LOCUS       DASUCJ010000319         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1424740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000319 DASUCJ010000000
VERSION     DASUCJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1126)
                     /locus_tag="VFU90_03840"
     CDS             complement(41..1126)
                     /locus_tag="VFU90_03840"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEU5478935.1"
                     /translation="MPQVFRHATPLVAAALAVGAIALARPLAAQYAAGANVGVSVVPI
                     TTGLVRVSADAGVGRNMSWTAPRTLLSLGSTAELGNKALGASLGFFAQRAAEADSEGP
                     VFAMRASAWHAIGPITLRLGLAEQALRFAGQPGTFDTAEVFVDSIRTDSGYVQYHGVT
                     VDTIRHPGTPSGVRIWSELEAGAQWVAGRVQLEAFVGARPAVASFPAAMWAQAGGSID
                     LPHGFGLQFAAGTSPARIGLGIPSTRFVSVGLRVRPMRASPETMAKPSAPIVSFAVHA
                     DGGRRFTFTYAGVSTGTVQISGDFTAWTPIDLTQDGHGRWRATVALTPGVHHVSLRVN
                     GGQWFAPPGIPAVPDDFGGTTGIIDVP"
     gene            1341..>1800
                     /locus_tag="VFU90_03845"
     CDS             1341..>1800
                     /locus_tag="VFU90_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478936.1"
                     /translation="MDSILRDVRYGFRSLIKSPGLTLVATLALTLGIGLTTVMFSMVY
                     GVIIKGLPYPNGDRIVEVIRNNKARGFNHMSVPISDYADFRNQQHTLAPVAAYYSGTV
                     NVSGKTQAERYTGSFVTASTFDVTGVQPVLGRTFRAGEDGPAGEKAVVISY"
BASE COUNT          294 a          620 c          606 g          280 t
ORIGIN      
        1 ttggtacctt cgcacccggg cggccgtcac gcgtcaccga ttatggaacg tcgatgatgc
       61 cggtggtgcc gccgaaatca tcgggcaccg cgggtatgcc gggcggcgca aaccactgac
      121 cgccgttcac gcggagactc acgtggtgca cgcccggcgt gagcgcgacg gtcgcgcgcc
      181 agcggccatg tccatcttgc gttaggtcga tcggcgtcca cgcggtgaag tcgccggaaa
      241 tctgtaccgt tccggtggac acgcccgcat acgtgaacgt gaatcgacgg ccgccatcgg
      301 cgtgcaccgc gaacgagacg atcggcgcag atggtttggc cattgtctcg ggcgacgccc
      361 gcattggccg cacgcgcagg ccgacgctca cgaaccgcgt gctcgggatg ccgagcccga
      421 tgcgcgcggg cgatgtgccg gcggcgaact ggaggccgaa gccatgcggc agatcgatgc
      481 tgccgccggc ctgcgcccac atcgcggcgg gaaacgatgc gacggccggt cgagcgccga
      541 caaatgcttc gagctgcacg cggcccgcga cccattgtgc gcccgcctcg agctccgacc
      601 agatgcggac acccgatggc gtgccggggt ggcggatcgt gtcgacggtc actccgtggt
      661 actgcacgta tccgctgtcg gtgcgaatcg agtcgacaaa gacctcggcc gtgtcgaagg
      721 tgccgggctg gccggcgaat cgcagcgcct gctcggcgag cccgagccga agggtgatcg
      781 gcccgatcgc atgccaggcc gatgcgcgca tcgcgaagac cggtccttcg gagtcggctt
      841 ccgccgcccg ttgggcgaag aagccgagcg atgcgcctaa cgccttgttc ccgagctccg
      901 cggtgctgcc taacgaaagg agggtgcgcg gcgccgtcca cgacatgttg cgtcccacgc
      961 cggcgtccgc gctcacgcgc accagtcccg tcgtgatcgg gaccacggac acgccgacat
     1021 tcgcgccggc cgcgtactgc gcggcgagcg gtcgcgcgag tgcaatcgcg cccacggcga
     1081 gagctgcggc cacgaggggc gttgcgtgtc gaaacacttg cggcatgacg actcctccgg
     1141 ttcgtgccgg cctcaggcaa gcatgtcgtc gtcgagcgtc gctggcaagc cgcccaaccg
     1201 aagccgggat cgcgcacccg cgcggatgta ctaccctcgc atcggcgcct cgcgtcacac
     1261 tgcgaaaccc tgtcgcaccc ggagccgtac gggcacgagt cggcctctcg tagcgaatcc
     1321 tcctgccgcc gggtctctcc atggattcga tcctccgtga tgtccgctac ggcttccgtt
     1381 ctctcatcaa gagtccgggg ctcacgctgg tcgccaccct ggccctcacg ctcggcatcg
     1441 gtctcacgac cgtcatgttc agcatggtgt acggcgtgat catcaagggg ctgccgtacc
     1501 cgaacggcga ccgcatcgtc gaagtgatcc ggaacaacaa ggcgcgtggc ttcaaccaca
     1561 tgtccgttcc gatcagcgat tacgccgact tcaggaatca gcagcacacg ctcgcgccgg
     1621 tcgccgcgta ctactccggg acggtgaacg tgagcggcaa gacgcaggcg gagcggtaca
     1681 cgggttcgtt cgtcaccgcg agcacgttcg acgtgaccgg cgtgcagccg gtgctcggcc
     1741 gcacgtttcg cgcgggcgaa gacggccccg ccggcgagaa ggcggtggtg atcagctacc
//
LOCUS       DASUCJ010000320         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1272085, whole genome shotgun sequence.
ACCESSION   DASUCJ010000320 DASUCJ010000000
VERSION     DASUCJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272085"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..218
                     /locus_tag="VFU90_03850"
     CDS             45..218
                     /locus_tag="VFU90_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478937.1"
                     /translation="MTRGRTLRKRLVEILLLLTAVFLLWILHVEIAYRQNVSRMPALL
                     NFHRSVITRLSRA"
     gene            complement(229..1422)
                     /locus_tag="VFU90_03855"
     CDS             complement(229..1422)
                     /locus_tag="VFU90_03855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU5478938.1"
                     /translation="MASNGRPEIVIVDGARTPFGNFGGALKDVSAIDLGVAAAKGALS
                     RSNVEPGKIDTIVFGNVIQTSADAIYGARHVGLKAGCPIHTPALIVNRLCGSGLQAIV
                     SGAQSLILGEATYVLAGGMENMSQSPHVIRGARWGLGLGQGKLEDALWEGLTDSYNGM
                     GMAITAENLAKRYEISREQADEFSLKSQAGAAKAIESGYFAEEIVPVEVPGKKGPTVI
                     DKDEGPRPGVTMEQLARLPARFIKDGIVTPGNASGITDGASAVVLTTARQAEKDGLQP
                     MGRLVSWGVVGVDPDIMGIGPAFAIPQAVERAGMKLDDMDLVEINEAFAPQVLACVDE
                     LGLDGSKLNPNGGAIALGHPLGASGARLVTTAMYQLLRTRGRYALCTMCIGVGQGIAM
                     VLERV"
BASE COUNT          259 a          529 c          507 g          302 t
ORIGIN      
        1 gatggtcgat tacgaggtta cgaacaacaa gtaggcggac gaatttgacg agaggtagaa
       61 cactgcggaa gcggctggtc gaaatattac tcctgctgac cgcagtgttt ctgctttgga
      121 tactgcacgt cgagatcgcc tatcgccaga acgtcagccg catgccggcc cttctcaact
      181 tccatcgcag cgtgatcacg cgtttgtctc gagcgtagcg agacatttct acactctttc
      241 cagaaccata gcgatacctt gtcccacgcc gatgcacatc gtgcacagcg catagcggcc
      301 gcgggtgcgc agcagttggt acatcgccgt cgtaacgagc cgcgcgccgc tcgcgccgag
      361 cggatggccg agcgcgatcg cgccgccgtt cggattgagc ttcgatccgt cgagcccgag
      421 ttcgtcgacg catgcgagaa cctgcggcgc gaacgcctcg ttgatctcga cgaggtccat
      481 gtcgtcgagc ttcatgcccg cgcgctcgac cgcttgcggg atcgcgaagg cgggtccgat
      541 gcccatgatg tcgggatcga cgccgacgac gccccacgac acgagccggc ccatcggctg
      601 caagccgtct ttttcggctt gtctcgcggt cgtgagcacg acggccgacg cgccgtcggt
      661 gatgccgctc gcgttgccgg gcgtgacgat gccgtctttg atgaagcgcg ccggcaatct
      721 cgcgagctgt tccatcgtga cgccggggcg cggcccttca tctttgtcga tgacggtcgg
      781 gcccttcttg cccggcacct cgacgggcac gatctcctcc gcgaagtagc cgctctcgat
      841 ggccttggcc gcacccgcct gcgacttgag gctgaactcg tcggcctgct cgcgcgatat
      901 ctcgtagcgc ttcgcgaggt tctcggccgt gatcgccatg cccatgccgt tgtacgaatc
      961 cgtgagacct tcccagagcg cgtcttcgag cttcccctgg ccgaggccga gaccccagcg
     1021 cgcgccgcgg atgacgtgcg gcgactggct catgttctcc atgccgccgg cgagcacgta
     1081 cgtcgcctcg ccgaggatga gcgactgcgc gccggagacg atcgcctgca gcccggagcc
     1141 gcacaggcgg ttgacgatga gcgccggcgt gtggatcggg cagccggctt tgagaccgac
     1201 gtggcgcgcg ccgtagatcg cgtccgcgct cgtctggatg acgttgccga agacgatcgt
     1261 gtcgatcttg cccggctcga cgttcgagcg cgaaagcgcc cccttcgcgg ccgcgacgcc
     1321 gaggtcgatc gcgctgacgt ccttgagcgc acccccgaag ttgccgaacg gcgttcgcgc
     1381 gccgtcgacg atgacgatct ccggtctccc gttgcttgcc ataacaatcc aatccgctct
     1441 tgtagtaggc cgagcgaagc tcggctgtgg gaggcttgtc ctttgtcgaa agggccgtgc
     1501 tgccctcgca cgcaatgccc gcgaccatgc ctcaggtcgc acggaagacc cgttgttcgt
     1561 gggcgacggg cgacgcaaga atggtcgaat accacga
//
LOCUS       DASUCJ010000321         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_309265, whole genome shotgun sequence.
ACCESSION   DASUCJ010000321 DASUCJ010000000
VERSION     DASUCJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309265"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFU90_03860"
     CDS             complement(<1..802)
                     /locus_tag="VFU90_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478939.1"
                     /translation="MNPLRGIIQQTSRFFTYPLTIAAVRLLAVVQTVAFPIGAVIPDW
                     LVFALLGYAVFAAILALPPRATPTSVEQKAALRPGGYGLLVALDVLATAVVAWKSGAS
                     TIAMLPALDAAVLGIGGAAALIAFASAAFATGMVLGQRGSIGGAFAQGLVLGVGPYLA
                     AAFSLIARRSAERQIRAINHVLDAGSDLGTKLAMAEVLTQLLNMLRQFRESVPWQNVV
                     VYVARYDEKAGEEVLCAEAISGSHADFYRGSKLRFSDGLVGYSATEQRP"
     gene            complement(828..>1120)
                     /locus_tag="VFU90_03865"
     CDS             complement(828..>1120)
                     /locus_tag="VFU90_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478940.1"
                     /translation="HFHELVGLSESFGLIENLHVAVADEQAGPVFSHRVLHGASSRSY
                     GIAVAKMAGLPAEVVARAQEIADEIESRPRLKSKPVRRRTAEGPDHQLRLTI"
BASE COUNT          205 a          373 c          364 g          178 t
ORIGIN      
        1 gcgggcgctg ctcggtcgcc gaatatccga cgagtccgtc cgagaatcgc agcttgctgc
       61 cgcggtagaa atcggcatgc gatccggaga tcgcctccgc gcagaggacc tcttcgcccg
      121 ccttctcatc gtagcgcgcg acgtagacga cgacgttctg ccacggcacc gattcgcgga
      181 actgacgcag catgttgagc agctgcgtca gcacctcggc catcgcgagt ttcgtgccga
      241 gatcggagcc tgcgtcgagg acgtggttga tggcgcggat ctgacgctcc gcgctgcgac
      301 gtgcgatgag cgagaacgct gcggcgagat acggcccgac gccgaggacc aagccttgcg
      361 cgaacgcgcc gccgatcgaa cccctttggc cgagcaccat gccggtcgcg aaagccgccg
      421 aagcgaatgc gatgagcgcc gcggcgccgc cgattccgag caccgcagca tcgagggcag
      481 gaagcatcgc gatcgtgctc gctcccgact tccacgcgac gaccgcagtc gccaatacgt
      541 cgagcgcgac gagcaacccg tagccgcccg gacgcagcgc cgccttctgt tcgaccgagg
      601 tcggtgtcgc ccgcggcggc agcgcgagga tcgccgcgaa gaccgcgtat ccgagaagcg
      661 cgaagacgag ccaatctggg atgaccgcgc cgatcgggaa cgcgacggtc tgcacgaccg
      721 cgagcaggcg cacggcagcg atcgtgagcg gatacgtgaa gaaacggctc gtttgctgga
      781 tgatgccccg caacgggttc atcggttctt taggggaccc cacttcgtca gatagtgagg
      841 cgcaattggt ggtccggacc ttcggccgta cggcgccgaa cgggcttgga tttcaatcgc
      901 ggcctgctct ctatctcatc ggcaatttcc tgtgctcttg ccacgacctc ggcgggcagc
      961 cccgccatct tcgccaccgc gatcccgtac gagcggctgc tcgcgccgtg aagcacgcgg
     1021 tgcgaaaaca caggccccgc ctgctcgtct gcgacggcga cgtggaggtt ttcgattaga
     1081 ccgaacgact ccgataaccc cacgagctcg tgaaagtgtg
//
LOCUS       DASUCJ010000322         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_645514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000322 DASUCJ010000000
VERSION     DASUCJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFU90_03870"
     CDS             <1..513
                     /locus_tag="VFU90_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5478941.1"
                     /translation="RAGTPLAGLLFGGGQESGRRAGTENLAGIVGLASALEPALAERE
                     AAGARIASLRDRLEAGVLDAVENVWVNGGAAERLPNVTNLSFAGVEPEALLARLDLEG
                     VAASAGSACTSGVLERSHVIAALGAPEWADAAIRFSLGGPTTQTEVDRVLEIVPPVVE
                     SLRRPTRIPA"
     gene            612..1028
                     /locus_tag="VFU90_03875"
     CDS             612..1028
                     /locus_tag="VFU90_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU5478942.1"
                     /translation="MTSSEIGVLVLDVLTGVGALLVGVGVLVGMLALAKTLKRVNVTL
                     DGLDRQLDNLGEPVAKTLGHVDGIADTADQTLARLGGVVQSLEDVAGSVSQTAQLAKN
                     ALSPAIVNVGAAVTGVSSGLRRLVTGKNSTDQPQQE"
     gene            1036..1386
                     /locus_tag="VFU90_03880"
     CDS             1036..1386
                     /locus_tag="VFU90_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478943.1"
                     /translation="MSNGKGGGFFAGFLLGSIVGGAAAVLLIQEETRDLLVGKAREAG
                     NFAKDATDDLRGRVNDVATQWQSSASDLYERGKSVVDSARSNIHAAVDEGKASAEQAR
                     DNLQEQAEGSNLGH"
     gene            1388..1720
                     /locus_tag="VFU90_03885"
     CDS             1388..1720
                     /locus_tag="VFU90_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5478944.1"
                     /translation="MQVREAPGDCYVVDLSGEIDVYTSPKVKDAIGELIDRGVYHLVI
                     NLEKVRYIDSTGLGVLIGGLKRVREHGGSVNLVCTNPQIRKIFDITGLVKIFGIFDNE
                     DAAMKALV"
     gene            1717..2151
                     /locus_tag="VFU90_03890"
     CDS             1717..2151
                     /locus_tag="VFU90_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5478945.1"
                     /translation="MNPAVEPATAPDLVELRIPCRPEWVALARLAAAAVANRLKFSIE
                     EIEDVKLAVAEACTAVIQHEKHGEFIELTCEARPDALRIRVHDAGRHLPAADRQAPMD
                     FDEARVAGLGVFLIRTLMDEVSYDVHPELGTDLRMTKRLIGR"
     gene            2210..>2463
                     /locus_tag="VFU90_03895"
     CDS             2210..>2463
                     /locus_tag="VFU90_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEU5478946.1"
                     /translation="MRFVEVRARHERDPEAPNPEYERLRDELVVAHLNLARFLAVKFA
                     NRGEPLDDLVQVGTVGLLKAIDRFDAARGVEFTTYATPTIV"
BASE COUNT          442 a          772 c          837 g          412 t
ORIGIN      
        1 cgcgccggca cgccgctggc cgggctgctg ttcggcggcg gtcaagaatc cgggcgccgg
       61 gccggcaccg agaatctcgc gggaatcgtg ggtcttgcga gcgcgctcga accggcgctg
      121 gccgagcggg aggcggccgg cgcgcggatc gcgagcctgc gcgaccggct cgaagccggg
      181 gtgttggacg ccgtcgaaaa cgtgtgggtc aacggcggcg ccgccgaacg gctgccgaac
      241 gtcaccaacc tttcgttcgc cggagtcgaa cccgaggcgt tgctcgctcg gctcgacctt
      301 gagggcgtgg ccgcctccgc cgggagcgcc tgcacgtcgg gcgtgctcga gcgcagccac
      361 gtgatcgccg ccctgggtgc gcccgagtgg gcggacgccg ccatccggtt ttcgctcggc
      421 gggcccacaa cccaaaccga ggttgaccgc gtcttggaga tcgtgccgcc ggtcgtcgag
      481 tccctgcgcc ggccgaccag gattcctgcg taagattcgc aggcgtaagg ggataagacc
      541 atagcggccc gaacggccag cgctcggcag ccgcgcaccc ggaaacagct tttgatagga
      601 gtagaaccgc ggtgacttcg tcggaaatcg gcgttttggt gctcgacgtg ctgacgggcg
      661 tgggggcgtt gctggtcggg gtcggcgtcc tggtcgggat gctggcgttg gcgaagacgc
      721 tcaaacgcgt caacgtgacc ctcgacgggc tcgatcgtca gctcgacaat ttaggcgagc
      781 cggtagcgaa aacgctcggt cacgtcgacg gaatcgccga taccgcggac cagaccttgg
      841 cgcgcttagg cggcgtggtg cagtcgctcg aggacgttgc gggcagcgtt tcccagactg
      901 cccagctcgc caagaacgcc ctctctccgg ccatcgtcaa cgtcggcgcg gcggtcaccg
      961 gcgtcagcag tggtttgcgg cgcctggtaa cgggaaagaa ctcaaccgac caaccccaac
     1021 aggagtaggt tttttatgag taacggcaag ggcggaggat tcttcgccgg ttttctgctc
     1081 ggatccatcg tcggaggagc cgcagcggtt ctgctcatcc aagaagaaac gcgcgacctg
     1141 ctggtcggga aagcgcggga agccggtaac ttcgcgaaag atgcgaccga cgatttgcgc
     1201 ggaagggtca acgacgtcgc cacccagtgg cagtccagcg cgtccgacct gtacgagcgc
     1261 ggtaagagcg tcgtcgatag cgcccgttcg aacattcacg ccgccgtcga cgaaggtaag
     1321 gcgagcgcgg agcaagctcg cgacaacctt caggagcagg ccgagggcag taatcttgga
     1381 cattaaagtg caggtgcgcg aagcgcccgg cgactgttac gtcgtcgatc tcagcggcga
     1441 aatcgacgtg tacacgtcgc cgaaagtgaa ggacgcgatc ggcgaactca tcgatcgcgg
     1501 cgtctaccac ttggtcatca atctggaaaa agtgcgctac atcgattcga ccggactggg
     1561 cgtactgatc ggcggtctca agcgcgttcg ggagcacggc ggttccgtca atctggtgtg
     1621 caccaatccg cagatccgca agatcttcga catcaccgga ctcgtgaaga tcttcggaat
     1681 attcgacaac gaagacgccg ccatgaaggc gctggtgtga accccgccgt cgaacctgcg
     1741 acggctcccg atctggtcga acttcgcatc ccctgccgcc ccgaatgggt ggctttggcg
     1801 cggctcgccg ccgccgccgt cgcgaatcgc ttgaagtttt cgatcgagga gatcgaagac
     1861 gtcaagctgg ccgttgccga ggcgtgcacc gccgtgattc agcacgaaaa acacggcgag
     1921 ttcatcgagc tcacctgcga agcccgtccc gacgcgctgc gtatccgggt gcacgacgcg
     1981 gggcgccacc tgccggccgc cgaccgccaa gcgccgatgg atttcgatga ggcgcgcgtc
     2041 gcgggcttgg gggttttcct gatccgcacg ttgatggacg aagtgagcta cgacgtgcat
     2101 cccgagttgg gaaccgatct gcgtatgacc aaaaggctga tcggccgcta gtccggtgta
     2161 cgaacgggac gattcgcgtc cggatcggga gcggacgcgg gcccttttct tgcgcttcgt
     2221 cgaggtgcgc gcgcgtcacg agcgcgatcc ggaggcgccc aatcccgaat acgaacgtct
     2281 gcgcgacgag ctggtcgtgg cccacctcaa cctcgcccgg ttcctggccg tcaagttcgc
     2341 caaccgcggg gagccgctcg acgatttggt gcaggtcgga acggtgggac tgctcaaggc
     2401 gatcgaccgg ttcgacgccg cgcgcggcgt cgagttcacc acctacgcca cgccgacgat
     2461 cgt
//
LOCUS       DASUCJ010000323         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1012200, whole genome shotgun sequence.
ACCESSION   DASUCJ010000323 DASUCJ010000000
VERSION     DASUCJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012200"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1542)
                     /locus_tag="VFU90_03900"
     CDS             complement(124..1542)
                     /locus_tag="VFU90_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5478947.1"
                     /translation="MVEHFDDLEEDFARVGRWIAGYFEHPEAYRVLPDVRPGELAARL
                     PAAAPERGEAFERILDDFERLIVPATTHWNHPRFFAYFATSAAPAAVAAEALAATLDV
                     KAMLWRTSPAATELEQVTMRWLGRLLGLPDSWTGIIYDTASIAGFTALAAARDALLPD
                     IRRRGMFELDRPLRVYVTEHTHSHIEKAAIALGIGQENVVPVACDAAFRMRPEALAER
                     VDADAADGMQPLATVATVGTTSTTAIDPLPAIADVTQRYGMWLHVDAAYAGMAAMLPE
                     FGDLLAGVERADSLVVNPHKWMFVPMDLSVLFLKDEATVRRAFSLVPEVLATPEGDEV
                     NYMNYGLQLGRRFRALKLWFVLRRYGAEGIRAKLREHIALAQTFAQWVRADSQWEVLA
                     PHPFSLVCFRYGAGDPAELDAFNERLMNAVNATGELFISHTKIDGRFALRLAIGNLRT
                     TRADVERAWEILRREAERLAAG"
     gene            1616..>2380
                     /locus_tag="VFU90_03905"
     CDS             1616..>2380
                     /locus_tag="VFU90_03905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5478948.1"
                     /translation="MKYRTYPNTDVTVSEVDFGLWTTSTGWWGNNTDDDAVAMLREAA
                     DLGITLFDAADTYGNGRSEEQLAKAFAGRRESVVYATKFGYDFYTPQNVEKRRGQFEL
                     PHDFSPAFVRKALEESLRRLQTDYVDIYQMHNARMAQIDDDALWELLESFKREGKIRM
                     YGVALGPAIGWLYEGVDAVEKRNVPSLQIIWNALEQYPGNEQIRAAAESGADTGYMIR
                     VPHSSGLLEGHYTEDTVFPDNDHRRHRPRSWLVNGVK"
BASE COUNT          438 a          821 c          768 g          353 t
ORIGIN      
        1 gcggccgcag ccgcgcagtc gaaggacgct gtgtcatcct gagcggagcg gccgcagccg
       61 cgcagtcgaa ggacgctgtg tcatcctgag cggagcggcc gcagccgcgc agtcgaagga
      121 cggctagccc gcagccaatc gctccgcttc ccgccgcaaa atttcccacg cgcgttcgac
      181 gtcggcgcgc gtcgtgcgga ggttgccgat cgccaagcgc agcgcgaacc ttccgtcgat
      241 cttcgtatgc gagatgaaga gctcgccggt cgcgttgacc gcgttcatca gccgttcgtt
      301 aaacgcgtcc agttccgccg gatcgccggc cccgtagcga aagcacacca gcgagaacgg
      361 gtgcggcgcg agcacttccc actgcgaatc cgcgcgcacc cactgcgcga acgtttgcgc
      421 gagcgcgata tgctcgcgca gttttgcccg gatgccctcg gcgccgtaac gccgcaggac
      481 gaaccacagc ttgagcgcgc ggaagcggcg ccccaactgc aacccgtagt tcatgtagtt
      541 gacttcgtcg ccttcgggcg tagccagaac ctcgggaacg agactaaacg cacgccggac
      601 ggtcgcttcg tctttgagaa acagcaccga caggtccatc ggcacgaaca tccatttgtg
      661 gggattgacg accagcgaat cggcccgctc gacgcccgcc agcagatcgc cgaactccgg
      721 gagcatcgcc gccatcccgg cgtaggccgc gtcgacgtgc agccacatcc cgtaccgctg
      781 cgtgacgtcc gcaatcgccg gcagcggatc gatcgcggtc gtcgaggtcg tgccgacggt
      841 cgcgaccgtc gccagcggct gcatcccgtc ggcggcgtcg gcgtctacgc gctccgcgag
      901 cgcttccgga cgcatccgga acgcggcgtc gcacgccacc ggcacgacgt tttcctgacc
      961 gatgccgagc gcgatcgccg ctttctcgat gtgcgaatgc gtgtgctcgg taacgtagac
     1021 gcgcagcggc cgatcgagct cgaacatgcc gcgtcgccga atatccggca ggagtgcgtc
     1081 gcgggcggcc gccagcgccg tgaaccccgc gatcgacgcg gtgtcgtaga tgatgcccgt
     1141 ccacgagtcc ggcaagccga gcagccgccc gagccagcgc atcgtgacct gctcgagctc
     1201 ggtggcggcc ggcgaggtac gccacagcat cgctttgacg tcgagcgtcg ccgccagcgc
     1261 ttcggcggcg accgctgccg gcgccgcgct ggtcgcaaag tatgcgaaga agcgcggatg
     1321 gttccagtga gtcgtcgccg ggacgatcag ccgctcgaag tcgtcgagaa tccgctcgaa
     1381 cgcttcgccg cgctcggggg cggccgccgg gagccgcgcg gccagctcgc cggggcggac
     1441 gtccggcagc acgcggtagg cttcggggtg ctcgaagtag cccgcgatcc atcgcccgac
     1501 ccgtgcaaag tcttcttcta agtcgtcgaa atgctccacc atggaacagg ttggcgggcg
     1561 gctcgcaaac gcccctgcgg cacgaatgcc ttaacggcga ggacaggttg catcgatgaa
     1621 ataccggacg tacccgaaca ccgacgttac cgtgagcgag gtcgacttcg gcctgtggac
     1681 gacctcgacc ggatggtggg gaaacaacac cgacgacgac gcggtcgcga tgctgcgcga
     1741 agcggccgat ctcggcatca cgttgttcga cgccgccgac acctacggca acggccgcag
     1801 cgaagaacag ctcgcgaaag cgttcgccgg acgccgcgag tcggtcgtct acgccaccaa
     1861 gttcggctac gacttctaca ccccgcagaa cgtcgaaaaa cggcgcggcc agttcgagct
     1921 gccgcacgat ttctcgcccg cgttcgttcg taaagcgctc gaagaatcgc tgcgccggct
     1981 gcagaccgac tacgtcgaca tctaccagat gcacaacgcg cggatggcgc agatcgacga
     2041 cgatgccctc tgggagctgc tcgaatcgtt caaacgcgaa ggcaagatcc gcatgtacgg
     2101 cgtcgcgctc ggccccgcga tcggctggct ctacgaaggc gtcgacgcgg tcgagaagcg
     2161 caacgtgccc agcctgcaaa tcatttggaa cgcgctcgaa cagtacccgg gcaacgagca
     2221 aatccgggcg gccgccgaaa gcggcgcgga caccggctac atgatccgcg tcccgcattc
     2281 gagcggcttg ctggaaggcc actacaccga agacacggtc tttccggaca acgaccaccg
     2341 ccgccaccgc ccgcgctcgt ggctggtcaa cggcgtcaag
//
LOCUS       DASUCJ010000324         1711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1180294, whole genome shotgun sequence.
ACCESSION   DASUCJ010000324 DASUCJ010000000
VERSION     DASUCJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180294"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VFU90_03910"
     CDS             complement(<1..1341)
                     /locus_tag="VFU90_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5478949.1"
                     /translation="MSQLAISPNGKAVLLITRIASERSRPTEVPKWVTKSGYVEDIKG
                     RDKVGDSQDSGRVALVSLPSGNARWLHAIPGDSIANRGLIRMLGWNDDGSRALLFTVR
                     SDYKERYIETVAEDGRMDTVDVLRDTAWIEGPCFGCGGWYDNGKRAWFVSEADGWAHL
                     YSVNADGSGRTQLTKGKWEVLDAVLSPDKKEFWMHTSEPSPYERQFFRMAVTGGARER
                     ITAAKGGHDVTVSPNGLLLADVYSTSNRPPELFVAPFRAGASLAQLTVSPTKAWLAYH
                     WIDPQIIQIPASDGVMIPARIYRPQDLGAQPNGAAVIFVHGAGYLHNVVDYWSSYYRE
                     YMFNQFLATNGYVVLDEDYRGSAGYGRDWRVAVYRHMGGRDLQDEVDASRYLGSKFGI
                     PPERVGIYGGSYGGFMTLMAMFTEPKHFGAGAALRAVTDWSHYNHWYTMRILNLP"
     gene            1342..>1711
                     /locus_tag="VFU90_03915"
     CDS             1342..>1711
                     /locus_tag="VFU90_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478950.1"
                     /translation="MLIFQQIQRLAADAFGARLLGLGDAVGAILVANHLEEALLVCLE
                     RFALRRVRRIFSWRRSRTDIGELLDEPSVQVGGKHVVVARKEHAAVADIELGVGLAGF
                     GVGETAYRLGFDIDDVDVALV"
BASE COUNT          268 a          523 c          609 g          311 t
ORIGIN      
        1 cggcagattc aggatccgca tcgtgtacca gtgattgtag tgcgaccagt cggtgaccgc
       61 gcggagcgcg gcgccggcgc cgaagtgctt gggttcggtg aacatggcca tgagggtcat
      121 gaagccgccg tagctgccgc cgtaaatgcc gacgcgctcg ggcgggatgc cgaacttgct
      181 gccgaggtag cgggaggcat cgacttcatc ctggagatcg cgtccgccca tgtgccggta
      241 gacggcgacg cgccaatcgc gcccgtagcc ggcggagccg cggtagtcct cgtcgagcac
      301 gacgtagccg ttggtggcga ggaactggtt gaacatgtac tcgcgataat agctggacca
      361 atagtcgacg acgttgtgca ggtagccggc gccgtggacg aagatgacgg cggctccgtt
      421 aggctgcgcg cccagatcct gggggcgata gatgcgcgcg ggaatcatga cgccatccga
      481 cgccggaatc tggatgatct gcgggtcgat ccagtgatac gcgagccacg ctttggtggg
      541 cgacaccgtg agctgcgcca gcgatgcgcc ggcgcggaag ggcgcgacga agagctccgg
      601 cggccggttg gacgtggagt agacgtcggc caggagcagg ccgttcggcg aaacggtgac
      661 gtcgtggccg cccttggcgg cggtgatccg ctcgcgcgcc ccgccggtga cggccatgcg
      721 gaagaactgg cgctcgtacg gcgacggctc gctcgtgtgc atccagaact ccttcttgtc
      781 gggcgacagc acggcgtcga gcacttccca cttgcccttg gtgagctgcg tgcggccgct
      841 cccatcggcg ttgacgctgt agagatgcgc ccagccgtcg gcttccgaca cgaaccaggc
      901 gcgtttgccg ttgtcgtacc agccgccgca gccgaagcac ggaccctcga tccaggcggt
      961 gtcgcggagg acgtcgaccg tgtccatgcg gccgtcctcg gcgacggtct cgatgtagcg
     1021 ctccttatag tctgagcgca ccgtgaacag cagcgcgcgc gagccgtcgt cgttccagcc
     1081 gagcatgcgg atgaggccgc ggttggcaat cgagtcgccg ggaatggcgt gcagccaacg
     1141 cgcgttgccg ctgggcaggc tgacgagggc gacgcggccg ctgtcctgcg agtcgccaac
     1201 cttgtcgcga cccttgatgt cttcgacgta tccgctcttg gtgacccact tgggcacctc
     1261 ggtgggtcgc gagcgctcgg acgcgatcct ggtgatgagc agcacggctt tgccgttagg
     1321 cgagatcgcg agctggctca cgtgctcatc tttcaacaga tacagcggct tgccgcggat
     1381 gccttcggcg cgcgccttct cggccttggc gatgctgtcg gcgcgatact cgtcgcgaat
     1441 cacctggaag aggcgcttct ggtctgcctc gagcgctttg cgctgcggcg agttcgccga
     1501 atcttctcgt ggcgccggtc ccggacggac atcggtgagc tgctggacga gcccagtgtg
     1561 caggtcggcg gcaaacacgt tgtcgtcgcg cgtaaagaac acgcggccgt cgccgacatc
     1621 gaactggggg tcggtctcgc gggcttcggt gttggtgaga cggcgtaccg tttgggtttt
     1681 gacatcgacg acgtagatgt cgccctggta g
//
LOCUS       DASUCJ010000325         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1440254, whole genome shotgun sequence.
ACCESSION   DASUCJ010000325 DASUCJ010000000
VERSION     DASUCJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440254"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFU90_03920"
     CDS             <1..889
                     /locus_tag="VFU90_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478951.1"
                     /translation="NAKPVDVFVESPGAAIAPQIAVDANGTIAIADNKDVRVFRPPLH
                     GFAPSSAKLTIRLTPAITEIGPGGADLVDVEFDPNLNLWLLNDLGGEISELRAPLSKS
                     SIAALTIGFGAPGSKTAGFSALIQARFDVSASLYVYANQANTGRSRVFKLSFPYAKPP
                     SPLGIDLAQADFVDGSQYLPTNPNPRPLALGQYFGPLQSPPPGSPPPLPVNELSQFAQ
                     PFNPVLGIFPDATIQTIVGALIADPPRSAVYTLDTATGRLDEYGLPLRSNARPKLSLP
                     CLGGPSNCDGKGEHLFLAP"
     gene            complement(1078..>2056)
                     /gene="clpX"
                     /locus_tag="VFU90_03925"
     CDS             complement(1078..>2056)
                     /gene="clpX"
                     /locus_tag="VFU90_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5478952.1"
                     /translation="KRVNMGATADDVELQKSNILLVGPTGSGKTYLAQTLAKILDVPL
                     AMADATSLTEAGYVGEDVENILLKLIQAADYDVKRAEKGIVYIDEIDKIARKSENPSI
                     TRDVSGEGVQQALLKILEGTTANVPPQGGRKHPHQEFIQIDTTNVLFICGGAFDGLER
                     IIESRVAANNMGFRAKPEGKREVRVGKLLQQLMPEDLLKFGLIPEFIGRLPIVVTLDA
                     LDELALRRILTEPRNALVRQFQKIMNMDGVDLQFTKEALVAIAIKAQSRKTGARGLRS
                     ILEETMLEVMYDLPSLQGVKKCIVNKDVIEKKESPTLIYHPEQKKDMPA"
BASE COUNT          336 a          686 c          616 g          418 t
ORIGIN      
        1 gaacgccaag ccggtcgacg tcttcgtcga atccccgggc gccgcgatcg cgccgcaaat
       61 cgccgtcgac gccaacggaa cgatcgcgat tgccgacaac aaagacgtgc gcgtttttcg
      121 tccgccgctt cacggcttcg cgccgtcgtc cgctaagctc accatccggc tgacccccgc
      181 gattacggag atcggtcccg gcggcgccga tctcgtcgac gtcgaattcg atccgaatct
      241 caacctgtgg ctgctcaacg atctcggtgg cgagatctcc gagctgcgcg ccccgctctc
      301 gaaatccagc atcgcggcgc tcacgatcgg gttcggcgcg cccggaagta agacggcggg
      361 cttctccgcc ttgatccagg cgcgcttcga cgtcagcgcc tcgctgtacg tctacgcgaa
      421 tcaagccaac accggccgct cgcgggtgtt caagctgagc tttccgtacg ccaaaccgcc
      481 gtcgccgttg ggcatcgact tggcgcaggc cgatttcgtc gacggcagcc agtatctgcc
      541 caccaacccc aacccgcggc cgctcgcgct cggccagtat ttcggaccgc tgcagtcccc
      601 gccgccgggt tcgccgccgc cgctgcccgt caacgagctc tcgcagttcg cgcagccgtt
      661 taatccggtg ttggggatat ttcccgacgc cacgatccag acgatcgtcg gcgctctaat
      721 cgccgatccg ccgcgcagcg cggtctacac gctcgacacc gccaccggac ggctcgacga
      781 atacgggctt ccgctacgtt ccaacgctcg tccgaagctc tcgctgccgt gtctcggcgg
      841 tccgtccaac tgcgacggca aaggcgaaca cctcttcctc gcaccatgat taaccgtgcg
      901 agtcgcggct aacgcttcgg tgacgggctt ctcgattttc gggcaaacct ggacggtttc
      961 ttcaaaactt cgttctcccg aaaatcgagc cgagcgagcg gaatcacagg acgtgtgaag
     1021 cgagcgtccc ggaaccgaag cgttaccgcg gcgagcggcg gatagtctgg ggcccgccta
     1081 cgcgggcatg tccttcttct gctcggggtg atagattagc gtcggcgact ctttcttctc
     1141 tatgacgtct ttgttgacga tgcacttctt gacgccttgg agcgacggca agtcgtacat
     1201 cacctcgagc atcgtctcct cgagaatcga gcgcagcccg cgggcgccgg tcttgcggct
     1261 ttgcgctttg atcgcgatcg cgaccagagc ttctttcgtg aactgcaggt cgacgccgtc
     1321 catgttcatg atcttctgga actggcggac cagcgcgttg cgcggctcgg tcaaaatgcg
     1381 gcgcagcgcc agctcgtcga gcgcgtcgag cgtcacgacg atcggcaagc ggccgatgaa
     1441 ctccggaatc aagccgaact tgagcaaatc ctcgggcatc agctgctgga gcagcttgcc
     1501 gacgcgcact tcccgtttgc cctcgggttt cgcgcggaag cccatgttgt tggccgcgac
     1561 ccgcgactcg atgatccgct cgagcccgtc gaacgcgccg ccgcagatga agagcacgtt
     1621 ggtcgtgtcg atctgaatga actcttggtg cggatgcttg cgtccgccct gcggcggcac
     1681 gttggcggtg gtgccttcca gaatcttgag cagggcctgc tgcacgcctt cgccggaaac
     1741 gtcgcgcgtg atcgacgggt tctcgctctt gcgggcgatt ttgtcgattt cgtcgatgta
     1801 aacgatgcct ttttcggccc gtttgacgtc gtagtccgcc gcttgaatca gcttgagcag
     1861 aatgttctcg acgtcttcgc cgacgtagcc ggcttccgtg agcgaggtgg catcggccat
     1921 cgccagcgga acgtcgagaa tcttcgcgag cgtctgcgcg aggtacgtct tacccgagcc
     1981 cgtcgggccg acgagcagaa tgttggactt ctgcagctcg acgtcgtccg cggtggcgcc
     2041 catgttcacg cgtttg
//
LOCUS       DASUCJ010000326         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1516628, whole genome shotgun sequence.
ACCESSION   DASUCJ010000326 DASUCJ010000000
VERSION     DASUCJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516628"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VFU90_03930"
     CDS             complement(<1..1464)
                     /locus_tag="VFU90_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5478953.1"
                     /translation="MNERRRVLGAFAAGIAGVLVPVRDAVYAATGAAPVPDDLAPAGT
                     VPAARPGLPPNLRWETNNDEPLIGSPKAIRGGTLRYELGAYPLTFRLMGPNSNDFFAA
                     WNRLFTFNFTLVQRHPVSDRYIPMMATHWSVQDDQRTIYFKLDPAARFSDGHPVTAAD
                     YVFTWQMMRSPHIVDPFYNTYAKQYYESVDRIDDHTLRIVGTRWSWRPLYDFAGLWAT
                     PAHATKLDADWVQRSNNEFQITVGPYVLKSVVRGESVTFGRVPNWWGEGRRYFIGQYN
                     FDRIELRVIPEGRSLDYLRRGELDVITEGSARAWHEDYDFPAVDNGWLRRARVMVDTP
                     AGVFGLHMNLEAPIFDNRHFRKAMQYLFNFGRINRNLMFDEYFRLVSFFEGTPFADRK
                     LKPYGFHPALARKELALAGYHRPAELRPRGFLGQLWNALRGIVFTRSDTADVLVNERG
                     EKASFSVIYGSKGLERHLTVIQQDFRRAGVDMQLRLLE"
     gene            complement(1457..>1750)
                     /locus_tag="VFU90_03935"
     CDS             complement(1457..>1750)
                     /locus_tag="VFU90_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5478954.1"
                     /translation="FRHLLPNCLTPLETYTTYAVVGAIFALTGHDNLGYGLPAPTPSW
                     GELIGQALEASNRDKLWLTLAPFCALTVTLVLVVFIGESVREAFDPKPYARYE"
BASE COUNT          293 a          551 c          610 g          296 t
ORIGIN      
        1 ctcgagcagg cgcagctgca tgtcgacccc cgcgcggcgg aaatcctgct ggatcaccgt
       61 gaggtggcgc tcgagcccct tgctgccgta gatcacgctg aagctcgcct tctcgccgcg
      121 ctcgttcacc agcacatcgg cggtatcgga gcgcgtgaag acgatgccgc ggagtgcgtt
      181 ccagagctgg ccgaggaaac cgcgcggccg cagctcggcc gggcggtggt agccggccag
      241 tgcgagctcc ttgcgtgcga gggcgggatg gaagccatag ggcttcagct tccggtcggc
      301 aaatggcgtg ccctcgaaga acgataccag ccggaaatac tcgtcgaaca tcaggttgcg
      361 gttgatacgc ccgaagttga acaggtactg catcgccttg cggaaatgcc ggttgtcgaa
      421 gatcggcgct tcgaggttca tgtgcaggcc gaagacgccc gccggcgtgt ccaccatcac
      481 gcgggcgcgc cgcagccagc cgttgtcgac ggccgggaag tcatagtcct cgtgccaggc
      541 gcgtgccgac ccctcggtga tgacgtcgag ctcgccgcgg cgcaggtagt cgaggctgcg
      601 cccctccggg atcacgcgca gctcgatgcg atcgaagttg tactggccga tgaagtaacg
      661 ccggccttcg ccccaccagt tgggcacgcg tccgaaggtt accgattcgc cgcgcacgac
      721 gcttttgagc acatacggcc cgacggtgat ctggaactcg ttgttgctgc gttggaccca
      781 gtcggcgtcg agcttcgtcg cgtgcgcggg cgttgcccac agcccggcga agtcatatag
      841 cggccgccag ctccagcgcg ttcccacgat gcgcagcgta tggtcgtcga tgcggtcgac
      901 cgactcgtag tactgcttcg cgtaggtgtt gtagaacgga tcgacgatgt gcggcgagcg
      961 catcatctgc cacgtgaaga cgtagtcggc cgccgtcacc gggtggccgt cgctgaagcg
     1021 cgcggcggga tcgagcttga agtagatcgt gcgctgatcg tcctgcaccg accagtgcgt
     1081 cgccatcatc gggatgtagc ggtcgctgac cggatgcctc tgaaccagcg tgaaattgaa
     1141 cgtgaacaga cggttccacg cggcgaagaa gtcgttggaa ttcgggccca tgaggcgaaa
     1201 tgtcagcgga taggcgccga gctcgtagcg aagcgtgccg ccgcgaatcg ccttcggcga
     1261 gccgatcagc ggctcgtcgt tgttggtctc ccagcgcagg ttcggcggca gccccggcct
     1321 ggcggcgggc acggtgcccg cgggggcgag gtcgtcaggg accggtgcgg cgccggtcgc
     1381 cgcgtacacc gcgtcgcgca ccggtaccag cacgccggcg atgcccgccg cgaacgcacc
     1441 gagcactcgg cggcgctcat tcatagcgcg catacggttt cgggtcgaac gcctcgcgca
     1501 ccgattcgcc gatgaacacg accagcacga gcgtcaccgt gagggcgcag aacggtgcga
     1561 gggtgagcca cagcttgtct cggttcgacg cttcgagagc ctggccgatc agctcgcccc
     1621 agcttggggt cggcgccggt agtccgtacc cgaggttgtc gtggccggtg agcgcgaaga
     1681 tcgcgccgac gacggcgtac gtggtatacg tctcgagcgg tgtcaggcag ttcggcagta
     1741 ggtgccggaa
//
LOCUS       DASUCJ010000327         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_309301, whole genome shotgun sequence.
ACCESSION   DASUCJ010000327 DASUCJ010000000
VERSION     DASUCJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1041)
                     /locus_tag="VFU90_03940"
     CDS             complement(<1..>1041)
                     /locus_tag="VFU90_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU5478955.1"
                     /translation="AAAHAARIAWVFNVRGSRNVWVADGPGFTGRQLTHYTGDDGMPI
                     ASLELTPNGNTVVYARGSETNAQGEVADPTSDVHQPEQQVWAADVSNGEPRLLGTMNC
                     AFEGCEDIQISPDGRFAVWSARHALWQAPISGQEPARQLAYIRGDNAEPRWSPDGKAI
                     AFTSDRGAYSLIGIYRFDSRTLQYVDPSVFRDMLPRWSADGSKLAFIRLTGGRGGFFS
                     GRLEPWSIWVWDAASGKTSQIWASTSDANGSYPGEWESDAFQFAAGNRIVFASEADGW
                     VHLYSIAAAGGAPLLLTPGAFSFQGGVALTPDGKTLLYASNQNDTDRRHIWRVDVDHP
                     GPVALTHGESIE"
BASE COUNT          155 a          352 c          366 g          168 t
ORIGIN      
        1 cattcgatgg actcgccatg cgtcagcgcg acagggccgg ggtggtcgac atcgacgcgc
       61 cagatgtggc ggcgatcggt gtcattctgg ttcgacgcat agagcagcgt cttgccatcg
      121 ggcgtgagcg ccacgccgcc ctggaaggaa aatgcgccgg gcgtgagcag cagcggcgct
      181 cctcccgcgg ccgcgatcga gtacaggtgg acccagccat cggcctcgga cgcgaagacg
      241 atgcggttcc cggcggcgaa ctggaacgcg tcgctttccc attcgccggg gtaggatccg
      301 ttcgcgtcgg acgtgctcgc ccagatctgg ctcgtttttc cgctggcggc gtcccagacc
      361 cagatcgacc acggctcgag gcggccggaa aagaatccgc cccggccgcc agttaggcgg
      421 atgaacgcga gcttcgagcc gtcggccgac cagcggggca gcatgtcgcg aaagacgctg
      481 ggatcgacgt actgcagcgt gcggctgtcg aaccggtaga taccgatcag cgaatacgcg
      541 ccgcggtcgc tcgtgaacgc gatcgccttg ccgtcgggcg accaccgcgg ttcagcgttg
      601 tcgccccgaa tgtaggcgag ctgccgtgcg ggttcctggc ctgaaatcgg cgcctgccag
      661 agcgcgtgcc gcgcgctcca gaccgcgaag cggccgtcgg gggaaatctg gatgtcctcg
      721 catccctcga atgcgcagtt catggtgccg aggagccgcg gctcgccgtt cgagacgtcg
      781 gccgcccaga cctgctgctc cggctggtgc acatcgctgg tcgggtcggc cacctcgccc
      841 tgggcgttcg tctcgctgcc gcgcgcgtag acgaccgtgt tcccgttagg cgtgagctcg
      901 aggctcgcga tcggcatgcc gtcgtcgccg gtgtagtgcg tcagctgccg gccggtgaag
      961 ccgggaccgt cggccaccca cacgttgcgc gatccgcgca cgttgaacac ccacgcgatg
     1021 cgcgcggcgt gcgccgcggc c
//
LOCUS       DASUCJ010000328         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1455430, whole genome shotgun sequence.
ACCESSION   DASUCJ010000328 DASUCJ010000000
VERSION     DASUCJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455430"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1442)
                     /locus_tag="VFU90_03945"
     CDS             complement(<1..>1442)
                     /locus_tag="VFU90_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5478956.1"
                     /translation="LTGAGFAYGAIIAAIALGVVLTYRGSGIVNFANGAIAMYVAYVY
                     SILRTRGELFLPPLPNPLKIFGSGAPNIPTAISFGADNMQFWPALLISLGFCVLLGLA
                     LHFLVFRPLRTAPPLAKVIASVGVFVFLQATIIRRYNTTSQVTPALGFVHKSTQVNLG
                     IFKMPADQLFVAVIVIVFATLLWALFKFTRFGLATRAAAENEKGAIVLGYSPDFLAGA
                     NWVLSMLITGLLGIFVASTQTSIDPLVLAALIVPALTAALVGSFASFGRTTAAAFVLG
                     MQIPLVQYISNKSWFPTYAGSAWPGFEYLLPLLGIVLVLFLAGSTLPERGAITSGRLP
                     FSPTPPKWAIRIAGPLLIVLTVLVGLFWFGPDYRLALTNTLVGIIICLSVVIITGFVG
                     QISLAQLSFSGIAAFTVSKLSADHGWPFPWPILAGAFAAMILGLLVALPALRVRGVNL
                     AIVTLGFAVAVDHVVFSNSAVTGGYNGAKV"
BASE COUNT          302 a          433 c          525 g          182 t
ORIGIN      
        1 ttcaccttgg cgccgttgta accgccggtg acggcgctgt tgctgaacac cacgtggtcg
       61 accgcgaccg cgaatccgag ggtgacgatc gcaaggttga cgccgcgcac gcgcagcgcc
      121 ggcaacgcga cgagcaaccc aaggatcatg gccgcgaacg cacccgcgag gatcggccac
      181 gggaacggcc agccatggtc ggcggagagt ttcgacaccg tgaacgcggc gatgcccgag
      241 aacgacaact gcgcgagcga gatctggccg acgaagccgg tgatgatcac caccgacagg
      301 cagatgatga tgccgacgag cgtgttggtg agggcgagcc ggtagtcggg accgaaccag
      361 aacaacccca ccaagaccgt gagcacgatg agcagcggcc cggcgatgcg gatcgcccac
      421 ttgggcggcg tcggcgagaa ggggaggcgg cccgaggtga tcgcgccgcg ctccggcagc
      481 gtgcttccgg cgaggaagag cacgagcacg atcccgagca gcggcaggag gtactcgaaa
      541 ccgggccatg cggagccggc gtaggtcggg aaccagctct tgttgctgat gtactggacc
      601 agcgggatct gcatgccgag cacgaacgcg gcggcggtcg tgcggccgaa tgacgcgaag
      661 ctgccgacga gcgcggccgt cagtgcgggc acgatgagcg ccgcgagcac gagcggatcg
      721 atggaggtct gcgtggacgc gacgaagatg ccaagcagac cggtgatcag catcgacagc
      781 acccagttcg ctcccgcgag gaagtcgggg gagtagccga gcacgatcgc gcccttctcg
      841 ttctccgcgg cggcgcgcgt cgcgaggccg aagcgcgtga acttgaacaa cgcccacagc
      901 aacgtcgcga agacgatcac gatgacggcg acgaacaact ggtcggcggg catcttgaag
      961 atgccgaggt tcacctgcgt cgacttgtgc acgaagccga gcgccggtgt cacctgtgaa
     1021 gtcgtgttgt accggcggat gatcgtggcc tggaggaaca cgaacacgcc gaccgacgcg
     1081 atgaccttcg ccagcggggg cgccgtgcga agcggtcgga acacgagaaa gtgcagcgcg
     1141 agcccgagca acacgcagaa gccgagcgag atgagcaacg ccggccagaa ctgcatgttg
     1201 tcggcgccga acgagattgc ggtcgggatg ttgggcgcgc cgctcccgaa gatcttcaaa
     1261 gggttgggga gtggcgggag gaacagctcg ccgcgcgtgc ggaggatcga atagacgtac
     1321 gcgacgtaca tcgcgatggc gccgttggcg aagttgacga tccccgagcc gcgatacgtc
     1381 agtacgacgc cgagcgcgat ggcggcgatg atcgcgccgt acgcgaaccc ggcaccggtg
     1441 ag
//
LOCUS       DASUCJ010000329         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1211054, whole genome shotgun sequence.
ACCESSION   DASUCJ010000329 DASUCJ010000000
VERSION     DASUCJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211054"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..748
                     /locus_tag="VFU90_03950"
     CDS             77..748
                     /locus_tag="VFU90_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5478957.1"
                     /translation="MIDDSSSTTPRRDFLGKVAAASIALGLGGALPERLNAAVDAVSP
                     DVEKWPDVHGKHRQLYDSVSWNNGFSLIWSMIFLDTNKASSNLADSDLNAVVVMRHEA
                     IPLAFTDPIWKKYKLGEAFKINDPATKAPAERNPFYNAKESELMFPGMAIEKLMQRGV
                     AFGCCNVALTVYSGMRAEAIGVDKATAKEEWVAGLIPGFTLLPSGVWGVNRAQEHGCT
                     YCYAS"
     gene            872..>1265
                     /locus_tag="VFU90_03955"
     CDS             872..>1265
                     /locus_tag="VFU90_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478958.1"
                     /translation="MRAVCISRHVFLGEHICSIMRSAGIECESVIGLREGMDASRVAR
                     PDVVICDYDLLATAPRHEWRRGSGRDDLPIVAVSLTRRPEEAHLVDGRAIAGFLYLPA
                     VDSAAVERTVLDATTRSARQRARDVEVPV"
BASE COUNT          226 a          400 c          406 g          233 t
ORIGIN      
        1 gcgcccttga cgttgcacga gtacctgccg cccacccccg cttctccgga tatcaacatc
       61 gctcaggagg cagcacatga tcgacgactc ttcctcgacc acgccccgcc gggattttct
      121 gggcaaggtc gcggccgcat ccattgcgtt aggcttgggc ggcgcgctcc ccgagcggtt
      181 gaacgccgcc gtcgatgcgg tctccccgga cgtcgagaag tggccggacg tccacggcaa
      241 acatcgccag ctgtacgact cggtcagctg gaacaacggc ttctcgctca tctggtcgat
      301 gatcttcctc gacaccaaca aagcgtcgag caacttggcg gacagcgacc taaacgcggt
      361 cgtggtcatg cggcacgaag cgattccgct cgcgttcacc gacccgatct ggaagaagta
      421 caagctcggt gaggcgttca agatcaacga tcccgctacg aaagcgcctg ccgagcgcaa
      481 tccattctac aacgcgaagg aaagcgagct gatgttcccc ggcatggcca tcgaaaaact
      541 gatgcagcgc ggggtggcgt ttggatgttg caacgtcgcg ctcaccgtgt acagcggaat
      601 gcgcgccgaa gccatcggcg tcgataaggc cacggcgaaa gaggaatggg tcgcgggtct
      661 cattcctgga tttacgctgc tgccgagcgg cgtgtggggt gtgaaccgcg cgcaggagca
      721 cggctgcacg tattgctacg cgtcgtagcc ggcgatatcg aaccgacggg cgggcgcaac
      781 ggcccgctcc gtcggatcgg ttcaccgcgg tgggaactct gtttcacagc tcggacgttt
      841 ggcgcggcca tcgtcgctat attgccgcat catgcgcgct gtctgcatct cgcggcacgt
      901 ttttttgggt gagcacatct gctcgatcat gcgaagcgcc ggcatcgagt gcgaatcggt
      961 gatcggcctg cgtgaaggga tggatgcgtc gcgcgtcgct cggcccgacg tcgtgatctg
     1021 cgattacgat ttgctcgcga cggcgcctcg tcacgaatgg cggcgcggat ccggccgcga
     1081 cgatctgcct atcgtcgctg tgagtctcac gcggcgacca gaagaagcgc acttggtcga
     1141 cgggcgcgcg atcgccggct tcctgtactt gcccgccgtc gacagcgctg ccgtcgagcg
     1201 aacggtgctc gatgccacga cgagatccgc gcggcagcgc gctcgcgacg tggaagtgcc
     1261 cgtgc
//
LOCUS       DASUCJ010000330         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_538250, whole genome shotgun sequence.
ACCESSION   DASUCJ010000330 DASUCJ010000000
VERSION     DASUCJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538250"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFU90_03960"
     CDS             complement(<1..435)
                     /locus_tag="VFU90_03960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU5478959.1"
                     /translation="MTAIGWLQAVVFFLIVLALTKPVGLYMARVFQGERTWLSPLFVP
                     IEKIAYRLGGVREDEEMTWYAYALSMLAFSVIGLVYLYVLLRTQKWLPFNPQHLDNMA
                     PDLAWNTAVSFLTNTNWQFYSGEATMSYLSQMAGLAWHNFVSA"
     gene            complement(448..537)
                     /gene="kdpF"
                     /locus_tag="VFU90_03965"
     CDS             complement(448..537)
                     /gene="kdpF"
                     /locus_tag="VFU90_03965"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEU5478960.1"
                     /translation="MPFDDVLGLVLTVAGLAYLIYAMLRPEKF"
     gene            867..1742
                     /locus_tag="VFU90_03970"
     CDS             867..1742
                     /locus_tag="VFU90_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478961.1"
                     /translation="MTVPARVLVSAALAALLLATGAVARAQPSTASPSPAPSPAPTPS
                     APSDPCGSLLSIVNRPTFGTGVCTVRTGHADIESGYTNLVTTGPGGGNTATYPQSLIR
                     IGTFDPHLDFEVGPISENRSSLGGAFAGGMSDLAIGAKYELGYTSNADWGVSTVVTIP
                     NGATAFSAGNAQYTGNFNWAYTLNSEFALSGTLGFNAFSGYNAGGVPQSYFAFTPTLE
                     LSAALPGGPSQVSAEYAYFSQAGPNLGSKSFFDFVYQRDFGQHVQFDVEYGFSPTLIN
                     GQKQHYVGAGLSFMN"
     gene            complement(1732..>2829)
                     /locus_tag="VFU90_03975"
     CDS             complement(1732..>2829)
                     /locus_tag="VFU90_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5478962.1"
                     /translation="LWQIAAVVALGVSFGLLEPGRFTYAFGQTTLYVFLPPLLFEAAW
                     NLNYNAIRRQWAAIATLAGPGVLLTASIVAGALGIVRVPFGPAFLTGAILSATDPIAV
                     IAVFKRMRVPTTLATIVECESLFNDAVAVALYRIALGALALGVAGGGAIAAIVLKTAA
                     GAVAGAAVGVAIAFVAAALLRGRGRVTLQIAATVVVAYGAYFAADALSLSGIFATIAG
                     GIALRYYERRWLSLRIADDVNRFWDILALVANVLVFFLVGAALQIGEIAREPVFVVAT
                     LIAIAVARVAVAGLLLPGGYPRGWLDVVRLAGMRGALSLALALALPASVPYRQAIVDA
                     TFAVALATLAASSLTVARAVRRTERRPRFSS"
BASE COUNT          561 a          941 c          909 g          418 t
ORIGIN      
        1 ggcggagacg aagttgtgcc acgcgaggcc ggccatctgc gacagatagc tcatcgtggc
       61 ttctcccgag tagaactgcc agttggtatt cgtcaggaag ctgaccgccg tattccaggc
      121 gaggtccggc gccatgttgt ccaaatgttg agggttgaac gggagccatt tctgcgtgcg
      181 caacaggacg taaagataga cgaggccgat caccgagaag gcaagcatcg agagcgcgta
      241 ggcgtaccac gtcatctcct cgtcttcgcg aacgccgcct agacgataag cgatcttctc
      301 gatcggaacg aacagcggcg agagccaggt gcgttcgccc tggaagactc gagccatata
      361 aagcccgacg ggcttggtca gcgccaagac gatcaggaag aacacgacgg cctgcagcca
      421 gccgattgcg gtcattgtcg agtcctgtta aaacttctcg ggacggagca tcgcataaat
      481 gaggtaggca aggccggcta cggtgagaac gaggccgagg acgtcgtcga acggcatggt
      541 gcgcaggcct aaaccttctc gcaaccgaga acgtagaggt caaggacgac gaaacagacg
      601 atcgagagta cgatgatacc gatctcgagc attatctcac gtgcgagctc ccggcgcggc
      661 aacggcttcg gctgggtgcc gatgcggacg tgcgttcaac atgacgactt caagataagc
      721 gctctcgccg gcgagcgcca gccgtcgaac ggatcatccg aacggatgat tgacagtccg
      781 tcgcgccggt gtaaatatag gcgacggctt cctctgcgac ctttgctaga ggcaatggcg
      841 tgcgcccgac ccgaagggag tattcgatga ccgtccccgc ccgcgttctc gtctccgccg
      901 cgctggcagc gttgctgctt gcgacggggg ccgttgcacg ggcgcagcca tcgaccgcat
      961 cgccctcgcc ggcgccttcg ccggcgccca ccccgtcggc accgagcgac ccgtgcggct
     1021 cgctgctctc aatcgtaaac cggccgacct tcggcaccgg cgtgtgcacg gttcgcacgg
     1081 gccacgccga catcgagagc gggtacacga atttggtcac taccggaccg ggcggcggaa
     1141 acaccgcgac gtatccgcag tcgttgatcc gcatcggaac cttcgacccg cacctcgact
     1201 tcgaggtggg tccgatcagc gagaaccgtt cctcgctcgg cggcgcgttc gccggcggaa
     1261 tgagcgattt ggcgatcggg gctaagtacg agctcggcta tacgtccaat gcggactggg
     1321 gcgtcagcac ggtggtaacc attccgaacg gcgcgaccgc cttcagcgcg gggaacgccc
     1381 agtacaccgg caacttcaac tgggcttaca cgctgaactc ggagttcgcg ctctcaggca
     1441 cgctcggctt caacgctttc agcggataca acgcgggcgg cgtgccccag tcgtacttcg
     1501 ccttcacccc gacgctcgag ctcagcgcgg cgctccccgg cggaccctcg caggtcagcg
     1561 cggagtacgc gtacttctcg caagccgggc cgaacctcgg ctccaagtcg ttcttcgact
     1621 tcgtctatca gcgcgacttc ggccagcacg tgcagttcga cgtcgagtac ggcttctcgc
     1681 cgacgctgat caacggccaa aaacagcact acgtcggcgc cggcctctcg ttcatgaact
     1741 aaatcgaggt cggcgctccg tgcggcgaac ggcccgcgcg accgtaaggc tgctggcggc
     1801 gagcgtggcg agcgcgaccg caaacgtcgc gtcgacgatc gcttgacgat acggaaccga
     1861 tgccggcagc gcgagcgcga gcgccagtga gagcgccccg cgcattcccg caagccgcac
     1921 gacgtccagc cacccgcgcg gatagccgcc gggcaggagc agtccggcca cggcgacccg
     1981 cgcgaccgcg attgcgatca gcgtcgcgac gacgaagacc ggctcgcggg cgatctcgcc
     2041 gatctgcaac gccgccccga ccaaaaagaa caccagcacg ttggcgacca aagcgaggat
     2101 gtcccagaac cggttgacgt cgtccgcgat ccgcagcgag agccagcgcc gttcgtagta
     2161 gcgcaacgcg ataccgccgg caatcgtcgc aaagataccc gacagcgaaa gcgcatcggc
     2221 ggcaaaatag gctccgtagg cgacgacgac ggttgcagcg atctgcagcg tcacgcggcc
     2281 gcgcccgcgc agcagcgccg ccgctacgaa ggcgatcgcg acccctaccg ccgctccggc
     2341 gaccgcgccc gcggcggttt tgagcacgat cgcggcgatc gcgccgccgc cggcgacgcc
     2401 gagcgcaagc gctccgagcg cgatccggta gagcgccacc gcgaccgcgt cgttgaagag
     2461 cgactcgcat tcgacgatcg tcgccagcgt cgtcggcact ctcatccgct tgaagaccgc
     2521 gatcaccgcg atcgggtcgg tggcggaaag gatggcgccg gtgagaaagg cgggcccgaa
     2581 cggcacgcgg acgatcccga gcgcacccgc tacgatcgac gccgtcagca gcacgccggg
     2641 accggcgagc gtcgcaatgg ccgcccactg gcggcggatc gcgttgtaat tgagattcca
     2701 cgccgcctcg aacagcagcg gcggcagaaa cacgtacagc gtcgtttggc cgaacgcata
     2761 ggtgaaacgg ccgggctcga ggagcccgaa cgatacgccg agcgcgacca ccgcggcgat
     2821 ctgccagag
//
LOCUS       DASUCJ010000331         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1455433, whole genome shotgun sequence.
ACCESSION   DASUCJ010000331 DASUCJ010000000
VERSION     DASUCJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455433"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1042
                     /locus_tag="VFU90_03980"
     CDS             102..>1042
                     /locus_tag="VFU90_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5478963.1"
                     /translation="MTRQGVRVEEVEAATARDAELRAGRRGTVVSVKRVRSVRMSGLK
                     YGDRQILLSKLSAMLASRVGASQALTLLRDTFTGRIKHVAHDLLMRVEAGASVPEAIS
                     AQGPRDFPSTVSAMIEAGANSGATPQALRDAVDFERNLNEIRRTSSRGIWGALVSFLV
                     GIVFVVGTVFGMVPKIMDSPLITMTGGSASFSRSIHFAHMIGYGTLTVLIVAILLGLL
                     GTVGRKIAPLFADAVILRIPLYKDIVLARGYFIAFYGLGLLVKAGVRMERALELMAES
                     TPPGALCADFRRARDAVKNGRPWAAVMRTLQPTDRAS"
BASE COUNT          164 a          356 c          341 g          181 t
ORIGIN      
        1 gaagcacgcc cgtatcttgc cgacgacgcc gaccatgcag cgcccgcggg acaagagccc
       61 ggaaaggtgg cggcgtaatg gcgcgctacg caacccgtgt gatgacccga cagggtgtcc
      121 gcgtcgagga agtcgaggcg gcaaccgcgc gcgacgcgga attgcgcgcc gggcgtcgcg
      181 gcacggtcgt ttccgtcaag cgcgtgcgca gcgtccgtat gtcgggcttg aaatacggcg
      241 accggcaaat tctcctgtcg aaactgtccg cgatgctcgc ctcgcgggtt ggcgcatcgc
      301 aagcattgac cctgctgcgc gacacgttca ccgggcgcat caaacacgtt gcgcacgact
      361 tgctgatgcg ggtcgaggcg ggcgcgagcg tcccggaggc catctccgcc caagggccgc
      421 gcgacttccc gagcacagtc tcggcgatga tcgaagcggg cgccaactcc ggcgcgaccc
      481 cgcaggcgtt gcgcgacgcg gtcgacttcg agcgcaatct caacgaaatc cgtcgcacgt
      541 cctcgcgcgg catttggggc gcgctcgtgt cgttcctcgt cggcatcgtg ttcgtcgtcg
      601 gaaccgtctt cgggatggtg ccgaaaatca tggacagccc gctcatcacc atgacgggcg
      661 gcagcgcgag tttcagccgc tccatccact tcgcccacat gatcggttac ggcacgctca
      721 cggtgctgat cgttgcgatc ctgctcgggc tgctcggcac cgtgggccgc aagatcgcgc
      781 cgctgtttgc cgacgcggtg atcctgcgca ttccgctgta caaggacatc gttctcgccc
      841 gcggctactt cattgcgttc tacggcctcg gcctcctggt caaggccggc gttcgcatgg
      901 aacgcgcgct cgaactgatg gccgaatcca cgccgcccgg tgcgctgtgc gccgactttc
      961 gtcgggcgcg cgacgccgtg aaaaacggcc ggccgtgggc tgccgtcatg cgaaccctgc
     1021 agccgactga ccgcgcgtcg tt
//
LOCUS       DASUCJ010000332         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1042860, whole genome shotgun sequence.
ACCESSION   DASUCJ010000332 DASUCJ010000000
VERSION     DASUCJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042860"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="VFU90_03985"
     CDS             <1..>1051
                     /locus_tag="VFU90_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEU5478964.1"
                     /translation="TYRVSGIADNTAQQPAGYYLTIGPKAYGTVPLPNAGDTIAIQGS
                     ATPSLDSIEAAIGGGPLLVKDGAWYADPDGPNKGEFLTHMPASAAGITSDGTLLLFEI
                     DGRQPALSIGVLQPQLASLMIAFGVVTGMQFDGGGSSTIVARLPGDMQASVLNSPSDG
                     VERRVADALLVYSDAPTGPPAKIFATPQVVRALPGARVPLHVAVTDAAEHAVRTCPCN
                     MRFRVIPASAGRVESGTFIAGDKPEDAVIRVQSGALQMDLPVHVTTAVARGEILPPHP
                     ALTKNGRLQLNARAFDKEGYPIALPATLPWVTSTGSIDGRGAFVASDRDAIASVRLGD
                     ALVRQQITVGEHAVP"
BASE COUNT          189 a          354 c          330 g          178 t
ORIGIN      
        1 cgacctatcg cgtctccggc atcgccgata ataccgcaca gcagcccgcg ggctattacc
       61 taacgatcgg gccgaaggcg tacggcaccg tgccgctgcc gaatgcgggc gataccatcg
      121 cgattcaagg cagcgcaacg ccgtcgctgg atagcatcga agccgcgatc ggcggcggcc
      181 cgctgctcgt caaagacggt gcatggtacg cggatccgga cggcccgaat aaaggcgagt
      241 ttctcacgca tatgccggcg agcgcggcgg gcatcacgag cgacggcacg ctgctgctct
      301 ttgaaatcga cggccgccag cccgcgcttt caataggcgt gctgcagccg cagctcgcct
      361 cgctgatgat cgcgttcggg gtcgtcaccg gtatgcagtt cgacggcggc ggcagctcca
      421 cgatcgtcgc gcgtttgccg ggcgatatgc aggctagcgt cttgaactcg ccgtcggacg
      481 gcgtcgagcg ccgcgttgca gatgctctgc tcgtttacag cgatgcgcca accggtccgc
      541 ctgcaaaaat ctttgcaacg ccgcaagtcg tccgggcgct ccccggcgcg cgcgttccgc
      601 tgcacgtcgc cgttaccgat gcagccgaac acgccgtgcg cacgtgtccg tgcaacatgc
      661 gctttcgcgt tattccggca agcgccggac gagtcgagag cggcacgttc atcgcaggcg
      721 ataaacccga agacgctgtg attcgcgtgc aaagcggcgc actgcaaatg gatctgcccg
      781 tgcatgtcac gacggcggtc gcacgcggcg agattctgcc gccgcatccc gcgctcacca
      841 agaacggccg cctacagttg aatgctcgcg cattcgacaa agagggctat ccgatcgcac
      901 tacccgccac acttccgtgg gtcacatcga cggggtcgat cgacggtcgc ggcgccttcg
      961 tcgcatcgga ccgcgacgca atcgcaagcg tgcgtctggg agacgcgctc gttcgacagc
     1021 agattaccgt cggcgagcac gccgtaccgt t
//
LOCUS       DASUCJ010000333         1327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_844272, whole genome shotgun sequence.
ACCESSION   DASUCJ010000333 DASUCJ010000000
VERSION     DASUCJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844272"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VFU90_03990"
     CDS             complement(<1..1093)
                     /locus_tag="VFU90_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5478965.1"
                     /translation="MPPNLRPLAQLFARQPLEWTASLDPKSPTLRRVLGWPALTAIGL
                     GTMLGGIFPTIGAGAHAAGPGVIVAYVLSGLVSVCVALCYAEFASMVPVAGSAYTYAY
                     ATLGELVAWVIGWDLILEYGLSVAPTASSLSDYFQHLLANVGIAFPAWAQTAHVAGPH
                     PQVDVCAALVTLAISALVAVGIRESAGVNGALVVVQIVAMLVFVAAVAHAVSPSNLHP
                     FAPFGYHGILTSTALVFFAYIGFDTVTVASEEAKNPERDVPIGIILALVLGGLLYVAL
                     TVCTVGVLRFDRLSDGAAMLDALGSVSKNHALYLVVAIGGIAGNTTVMLTSLLGQVRI
                     FYVMARDRMLPPGIARVHHVFRTPARMTAI"
     gene            complement(1107..>1327)
                     /locus_tag="VFU90_03995"
     CDS             complement(1107..>1327)
                     /locus_tag="VFU90_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478966.1"
                     /translation="DVEAQKAITICGKQPAQYFKARGSLKGNSNSNVEMVMTNAGGTT
                     YMAMYVYPNGVQTNADAEAALRELCTKP"
BASE COUNT          264 a          435 c          433 g          195 t
ORIGIN      
        1 tgatcgccgt cattcgggcg ggcgtgcgaa aaacgtgatg gacgcgcgcg attccgggcg
       61 gaagcatgcg atcgcgcgcc atgacgtaga atatccgtac ttgtccgagc agcgacgtca
      121 gcatgacggt ggtgttgccc gcgatccctc cgatcgcgac caccaaatac agcgcgtggt
      181 tcttgctgac gctgccgagc gcgtcgagca tcgcggcacc gtccgaaagg cggtcgaagc
      241 gcaggacgcc gacggtgcac accgtcagag cgacgtacag cagtccgccg agaacgagcg
      301 ccaagatgat cccgatcgga acgtcgcgct cgggattctt cgcctcctcg gaagcgaccg
      361 tcacggtgtc gaaaccgata tacgcgaaga ataccagcgc cgtactggtc aagatgccgt
      421 gatagccgaa cggcgcgaac ggatggagat tcgacggcga aaccgcatgc gcgaccgccg
      481 ccacgaacac cagcatcgcg acgatctgca ccacgaccag cgcgccgttg acgccggccg
      541 actcgcgaat gccgaccgcg accagcgcgc tgatcgcgag cgtcaccaag gcggcacaga
      601 cgtcgacctg cggatgcggg ccggcgacgt gcgcggtttg cgcccaagcc ggaaacgcga
      661 tgccgacgtt agcgagcaag tgctggaaat agtccgacag cgacgaagcc gtcggcgcga
      721 cggagaggcc gtactcgagg atcaggtccc agccgataac ccaggcaacc agttcgccca
      781 gggtcgcgta cgcatacgta taggcgcttc ccgcgaccgg caccatcgac gcaaactccg
      841 cgtaacaaag cgccacgcag acgctgacca gccccgacag gacgtaggcg acgatgacgc
      901 ccggcccggc cgcgtgggcg ccggccccga tcgtcggaaa aatcccgccc agcatcgttc
      961 ccaagccgat ggcggtgagg gccggccagc ccaagacgcg ccgcagcgtc ggacttttcg
     1021 ggtcgagcga ggcggtccac tcgagcggct gacgcgcgaa gagctgcgcg agcggacgga
     1081 ggttcggagg caagcgaagc ggcggtctac ggcttcgtgc agagttcccg caacgcggcc
     1141 tcggcatccg cgttggtttg cacgccgttc ggatagacgt acatcgccat gtacgtcgtg
     1201 ccgccggcgt tagtcatgac catctcgacg ttgctgttgc tgttgccctt gagcgaaccg
     1261 cgagccttga aatattgcgc cggctgcttc ccgcagatcg tgatcgcttt ttgcgcctcg
     1321 acgtcgg
//
LOCUS       DASUCJ010000334         1919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1409605, whole genome shotgun sequence.
ACCESSION   DASUCJ010000334 DASUCJ010000000
VERSION     DASUCJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409605"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFU90_04000"
     CDS             complement(<1..681)
                     /locus_tag="VFU90_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478967.1"
                     /translation="MSCRRLPRSAARMMQHVRRSLIGFPALVLAVASVQAQGTRFLRR
                     PTVSHDMVAFEYGGDLWVVPRAGGAARRLTSTPSVETDPAFSPDGSMLAYTATVAGNA
                     DVYVVPVAGGEPKRLTYHPGGDFVRGWTPDGKKVVFASSRGTVPTPGANSFLRMWTVA
                     STGGMPEQVPMPRAFAGSYSSDATHMAYQPLGVAFFAANWAEPQYSQWRRYRGGRTEP
                     IHIMDLANY"
     gene            complement(714..>1919)
                     /locus_tag="VFU90_04005"
     CDS             complement(714..>1919)
                     /locus_tag="VFU90_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEU5478968.1"
                     /translation="DITTLEQSNPGQLGPDVFAPKNADLIVNSSRGDFECAFNEYIAA
                     SGLFMDEMADAISQTANFDLDRRSITPDSPYGTNTCDSQQQPGVYTPLSVARASNDVA
                     VQHLEGWTDEQVPDRSHLIAVASAYGGYSFVLMGEGMCSAAFDLGPEEQPQQIFGDAM
                     VRFDTAVAAATRANDATTLNLALLGRARTELDLGNSADAATDAALIPAGFEVDIDHDP
                     TATRRQNLVFIQTIQAQFGSIDTSIINRFVVDSDPRIAVTSTGELGSDGHTVVWFANK
                     DATATAPQALAKFSEAQLIIAENDVNTGDLNDAVTILNTLRSAAHQPAYSGGLTAPAV
                     MADIVEQRRREFFLEGHRLGDIRRLGLPLSPATGAPYVNGGTYADQACFPLPNVERIN
                     NPNLDRAAR"
BASE COUNT          313 a          611 c          655 g          340 t
ORIGIN      
        1 gtagttggcg agatccatga tgtgaatcgg ctcggtgcgt ccgccgcggt agcgtcgcca
       61 ctgactgtac tgcggctccg cccagttcgc cgcgaagaac gcaacaccga gcggttgata
      121 cgccatgtgc gtcgcatcgg acgagtacga gccggcgaac gcgcgcggca ttggcacctg
      181 ctcgggcatg ccgccggtcg acgcgaccgt ccacatgcgc aggaacgaat tggccccggg
      241 cgtcgggacc gtgccgcgcg aggatgcgaa caccaccttc ttgccatcgg gcgtccatcc
      301 gcggacgaag tcgccgcccg ggtggtacgt taggcgtttg ggctcgccgc cggcgacagg
      361 aaccacgtaa acgtcggcgt tgcctgccac cgtcgccgtg taggcgagca tcgatccgtc
      421 cggcgagaat gccggatcgg tttccacgga cggcgtgctg gtgagccgtc gcgcggcgcc
      481 tcccgcgcgc ggcaccaccc acaagtcgcc gccgtattcg aacgcgacca tgtcatggct
      541 gacggtcggc cgtcgcaaga aacgcgtgcc ctgtgcctgc acggaagcga ccgcgagcac
      601 gagcgccggg aatccgatca gcgatcgccg cacgtgctgc atcatccgtg cggcgctgcg
      661 cggcagtctt cgacacgaca tgtccgatct cctggagtac gaagtgaaag gcgtcaacgg
      721 gcggcgcgat cgagattcgg gttgttgatg cgctccacgt taggcagcgg gaagcacgcc
      781 tggtcggcat acgtgccgcc gttcacgtac ggggcgccgg tggcggggct caacggcagc
      841 ccgagccgcc ggatgtcacc gagccggtgt ccctcgagga agaactcgcg gcggcgctgt
      901 tccacgatgt ccgccatcac cgcgggagcc gtaaggccgc cgctgtaggc cggctggtgg
      961 gcggcactgc gtaatgtgtt caggatggtc accgcgtcgt tcaggtcgcc cgtgttgacg
     1021 tcgttctccg cgatgatgag ttgcgcttcc gagaacttgg cgagtgcctg cggcgcggtt
     1081 gccgtcgcgt ccttgttcgc aaaccaaacc acggtgtggc cgtccgagcc cagctcgccc
     1141 gtcgacgtca cggcgatccg cgggtcgctg tccacgacga accggttgat gatcgacgta
     1201 tcgatcgacc cgaactgcgc ctggatggtt tggatgaaca ccaggttttg gcgccgcgtc
     1261 gccgtcggat cgtgatcgat gtcgacctcg aagcctgcgg gaatcagtgc cgcatcggtc
     1321 gccgcgtccg ctgaattccc gagatccagc tcggtccgcg cacgaccgag cagcgcaaga
     1381 ttgagcgtcg ttgcatcgtt cgcgcgcgtc gcagcagcga cggccgtgtc gaatctgacc
     1441 atcgcatcac cgaagatctg ttgcggctgc tcctcggggc cgaggtcgaa tgccgcggag
     1501 cacatgccct cgcccatgag cacgaagctg tagccgccgt atgcggacgc cacggcgatg
     1561 aggtgagagc ggtcgggaac ctgttcgtcg gtccatccct cgaggtgctg caccgcgaca
     1621 tcgttcgaag cgcgcgcgac cgagagcggc gtgtagacgc cgggctgctg ctggctgtcg
     1681 caggtgttcg ttccgtacgg ggaatcgggg gtaatcgagc ggcgatcgag atcgaaattc
     1741 gcggtctgtg agatcgcgtc ggccatttcg tccatgaaca atccgctcgc cgcgatgtac
     1801 tcgttgaacg cgcactcgaa gtcgccgcgc gagctgttca cgatgaggtc cgcgttcttc
     1861 ggtgcgaaca catcggggcc taactgaccg gggttcgact gctcgagggt ggtgatgtc
//
LOCUS       DASUCJ010000335         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_431507, whole genome shotgun sequence.
ACCESSION   DASUCJ010000335 DASUCJ010000000
VERSION     DASUCJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431507"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFU90_04010"
     CDS             complement(<1..184)
                     /locus_tag="VFU90_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478969.1"
                     /translation="MTRNRLCAVMAVAVLYAAAASAQEAAPAAAPDLKKAQQTATTVC
                     AACHGPDGNSTVPANPN"
     gene            233..970
                     /gene="yihA"
                     /locus_tag="VFU90_04015"
     CDS             233..970
                     /gene="yihA"
                     /locus_tag="VFU90_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU5478970.1"
                     /translation="MVKLVRAKPSVSRAKLSILAKRPVTAKARGASARPAAGSKRPAA
                     APAPLVGAAFETGAVAAEQFPGSGEREIAFAGRSNAGKSSAINALARHRGLARASRAP
                     GRTREINFFRLRGGTRVADLPGYGYAAVSDTLKQAWQELLWTYVSARTQLVGLVLVAD
                     ARRGLSELDRRLLDVYLPSGRPALILATKMDKLGSSARRAAAAAILAQARLDHPTGGG
                     RITVVAFSATQPLGLEAADAVLAEWTA"
     gene            1191..>2023
                     /gene="hemB"
                     /locus_tag="VFU90_04020"
     CDS             1191..>2023
                     /gene="hemB"
                     /locus_tag="VFU90_04020"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU5478971.1"
                     /translation="MRRMRRHDFSRRLARETRLAADDFIYPVFVCEGSQHAEPVTSLP
                     GVSRKSIDLVLTDAERCVELGVPAIALFPVVPADLKSDDAAAAFDDAGLVPCTIRALK
                     SRFPKLGVVTDVALDPYTRHGQDGLIDASGYVVNDETVAALVKQALCHARAGVDIVAP
                     SDMMDGRIGALRKALDDARHLDVRILAYSAKYASAFYGPFRDAVGSAAGLGGGDKYSY
                     QMDPANGNEALREVFLDIGEGADMVMVKPGLPYLDIVRRVKDAFAMPTFVYQVSGEYA
                     M"
BASE COUNT          316 a          675 c          692 g          340 t
ORIGIN      
        1 gattcggatt agcgggcacg gtgctgttgc cgtcgggccc gtggcatgcc gcacagacgg
       61 tcgtcgcagt ctgctgtgcc ttcttgagat caggcgcggc ggcgggcgca gcctcctggg
      121 cggatgcagc ggcggcgtac agcacggcca ccgccatgac ggcacagagg cggttgcggg
      181 tcatgggcaa agctccattc ttcgattcgt tgtgcagggc cgcccgaaat ccgtggtgaa
      241 gctcgtgcgg gcgaagccat cggtgtcgag ggctaaacta tctattctag cgaaacgccc
      301 cgtcacggcg aaggcccggg gtgcatcggc gcggcccgct gccggatcga aacggcccgc
      361 cgccgcgccg gcgccccttg tcggtgccgc gttcgagacc ggcgccgtcg ctgcggaaca
      421 gtttcccgga tcgggcgagc gcgagatcgc gttcgccggg cgctcgaacg ccgggaagtc
      481 gagcgccatc aacgcgctcg cgcggcaccg cggtcttgcc cgcgcaagtc gggcgcccgg
      541 acgaacgcgt gagatcaact tcttccggct gcgcggcggt acgcgggtgg cggatctccc
      601 cggctatggc tacgccgccg tatcggatac cctgaagcag gcgtggcagg agctgctgtg
      661 gacgtatgta agtgctcgca cccagcttgt cgggctggtg ctcgtcgccg atgcgcgccg
      721 cggcctttcc gagctcgacc gccggttgct cgatgtctat ctgccgagtg gtcgtcccgc
      781 gctgattctc gccacgaaaa tggacaagct cgggtcctcg gcgcgccgtg cggcggccgc
      841 ggccatactc gcgcaggcgc gcctcgatca cccgaccggc ggcgggcgca tcaccgttgt
      901 cgccttctct gcgacgcagc cgttggggct cgaagcggcc gacgcggtgc tggcggaatg
      961 gaccgcgtga gcgcaaaaag aaaaggcccc gtggtcaagg ggagtgacct ggggcctcga
     1021 aacgccgaca ccagcgaggc tggcgtcggc acccgctcag ggaggaaagc gggagacgta
     1081 aaccgtccga tcatgagact cgcgccgcga cgtcgagttc cgtcgccgca gcatccgtac
     1141 cggagaatgc aatgacgacg agctttgccg ggaccttccc ccgaacgcgc atgcggcgca
     1201 tgcgccgcca tgatttttcc cggcgcctcg cgcgcgaaac gcgcctcgcc gccgacgatt
     1261 tcatctaccc ggtattcgtc tgcgagggca gtcagcacgc cgagcccgtg acgtcgcttc
     1321 cgggcgtgtc gcgcaagtcg atcgacctgg tcctcaccga cgccgagcgc tgcgtcgaac
     1381 tcggcgttcc ggccattgcg ttgtttccgg tcgttccggc cgacctcaaa tcggacgatg
     1441 ccgctgccgc attcgacgat gcggggctcg tcccgtgcac catccgcgcg ctgaaatcgc
     1501 gcttcccgaa gcttggcgtc gtcaccgacg ttgcgctcga tccctatacc cgccacgggc
     1561 aggatggact gatcgatgcg agcggttatg tcgtcaacga cgaaaccgtc gcggcgctcg
     1621 tcaagcaggc gctttgccac gcgcgggcgg gcgtcgacat cgtcgcgccg tccgacatga
     1681 tggacgggcg catcggcgcg ctgcgcaaag cgctcgacga tgcgcgccat ctcgacgtgc
     1741 gcatcctcgc ctattcggcg aaatacgctt cggccttcta cggcccgttc cgcgacgcag
     1801 tgggctcggc agcaggcctc ggcggcggcg acaagtattc gtatcagatg gacccggcca
     1861 acggcaacga ggcgctgcgc gaggtgttcc tcgatatcgg cgaaggcgcc gacatggtga
     1921 tggtgaagcc gggattgccg tacctcgaca tcgtgcgccg cgtgaaggac gcgttcgcga
     1981 tgccgacgtt cgtctaccag gtctcgggcg aatacgcgat gat
//
LOCUS       DASUCJ010000336         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1104034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000336 DASUCJ010000000
VERSION     DASUCJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFU90_04025"
     CDS             <1..418
                     /locus_tag="VFU90_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HEU5478972.1"
                     /translation="AGDVLWFERPFDLAHEWSHDAAYAREDEANYLAIVSCLRSPDPA
                     IRYSGWLELFLYLPQKRHYARHEFGPLVWQDFEALRLRNSRHVNVLLAHWTWRTYNAY
                     LKTNRVAGGVENYNEVTRLMLGIPLDRAGLPEARGT"
     gene            415..981
                     /locus_tag="VFU90_04030"
     CDS             415..981
                     /locus_tag="VFU90_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478973.1"
                     /translation="MNPFDYLTVLVSIVLGLAIANVLTGLATIMHARERVTFYWPPVA
                     WAIWLFFVAAEHWWAEWGVHNARSWNFGSFLLELLVPVDLFLLASLVLPPRDPQGRID
                     LYAWYARNRAWFFGLLFCLPLLSIAEELSRTGRMESIVNLYFLLAFAAVALLGWIWKS
                     RRAHEWITAQTMVLTLVYVGVLYFQLSR"
     gene            1000..1623
                     /locus_tag="VFU90_04035"
     CDS             1000..1623
                     /locus_tag="VFU90_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5478974.1"
                     /translation="MIYNQIAGRSVGRLAALSDGVFAFAMTLLVLDLRVPAAELVHSE
                     HDLLRQLAALTPNVLTYFMSFMTLGMFWVGQQTQLNKVDHSDRDFAWLHLAFLLAVTM
                     VPFSTKLLAAFIAYRTALLIYWLNILALGATLWLSWRYVRRAKLLKADAPSEVDPAIV
                     RRIAIAQALYAFGAALCVFNTYVSSGFIILVQLNYVLAPKIGALRRL"
     gene            complement(1618..2244)
                     /locus_tag="VFU90_04040"
     CDS             complement(1618..2244)
                     /locus_tag="VFU90_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5478975.1"
                     /translation="MNKSRFEAFSDGVFAFAITLLVLGFVLPELHDPNSNRELASALL
                     RMWPNLLAYALSFGVIGIMWQNHHALFRLVRAIDRTTVFLNLLLLGGTAFIPFATNAL
                     GAHPTLPAATFLYGVTLSFCSTMYNLLLGHLVGARAFDPAVEATSIARTVRAYRTGWV
                     GYVAATVLALVVPVVSFAAFLAIAAYYLIPHGVDADAASEPAGGRPDS"
     gene            2310..2549
                     /locus_tag="VFU90_04045"
     CDS             2310..2549
                     /locus_tag="VFU90_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478976.1"
                     /translation="MRHTLGAMFDVGESLRGALETARERLNDAQRAVARANAGGDAGR
                     SAGAAMAATAQAAIFAEALLGAERARLQEIKAVTR"
     gene            2546..>2874
                     /locus_tag="VFU90_04050"
     CDS             2546..>2874
                     /locus_tag="VFU90_04050"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEU5478977.1"
                     /translation="MIELLDAAASAMDAQRAALDVAARNVAAAEAAGPDGAYAREVPE
                     FTVIAGAGGEADRVAFAGSHLERGTNVDILTEMIAVMNASRAYESNASLFDVGKTLAE
                     KTIELGRL"
BASE COUNT          480 a          920 c          972 g          502 t
ORIGIN      
        1 ggccggcgac gtgctgtggt tcgagcggcc gttcgatctg gcgcacgagt ggagccacga
       61 cgccgcttac gcgcgcgaag acgaagccaa ttacttggca atcgtcagct gtttgcgttc
      121 ccccgatccg gcgatccggt actccggctg gctcgaactg tttctctacc tgccgcagaa
      181 gcggcattac gcgcggcacg agttcgggcc gctggtctgg caagacttcg aagccctgcg
      241 tctgcgcaac tcgcgtcacg tcaacgttct gctcgcgcac tggacgtggc gcacttataa
      301 cgcctatctc aagaccaacc gcgtcgccgg cggcgtcgaa aactataacg aggtaacgcg
      361 cttgatgctc ggcattccgc tcgaccgcgc cggcttgccg gaggcccgcg gtacgtgaac
      421 ccgttcgact atctcaccgt cctcgtttcg atcgtgctgg ggctggcgat cgccaacgtc
      481 ctcaccggtc tggcgacgat catgcacgcg cgcgaacggg taacgttcta ttggccgccg
      541 gtcgcgtggg caatctggct gttcttcgtg gccgccgaac actggtgggc cgagtggggc
      601 gtgcacaacg cccgcagctg gaacttcgga tccttcctgc tcgagctgct ggtgccggtg
      661 gacctgttct tgctggcgtc gctggtgctg ccgccgcgcg atccgcaggg gaggatcgat
      721 ctctacgcgt ggtacgcgcg caaccgggcg tggtttttcg ggctgctgtt ctgcctcccg
      781 ctgctcagca tcgccgagga gctgtcgcga accggccgga tggagtcgat cgtcaacctg
      841 tactttctgc tcgcgttcgc cgcggttgcg ctgctggggt ggatctggaa gtcgcgccga
      901 gcgcacgaat ggattaccgc gcaaacgatg gtattgacgc tggtgtacgt cggcgtcctg
      961 tactttcagc tttcgagata agcacgtgag gcggaaccgg tgatctacaa tcaaattgcg
     1021 gggcgcagcg tcgggcgcct cgcggcgctc agcgacggcg tcttcgcctt tgcgatgacg
     1081 ctgctggtgc tcgatttacg cgtacccgcc gccgagctgg tgcactccga gcacgacttg
     1141 ctgcgccagc tggcggcgct cacccccaac gtgctcacct acttcatgag cttcatgacg
     1201 ctgggcatgt tctgggtcgg ccagcagacg cagctcaaca aggtcgacca ctccgaccgc
     1261 gacttcgcct ggctgcactt ggcgttcttg ctggcggtaa cgatggtgcc gttttcaacc
     1321 aagctgctgg cggcgttcat cgcgtaccgc acggcgctgc tgatctactg gctcaacatc
     1381 ctggcgctgg gcgcgacgtt gtggctgagc tggcggtacg ttcgccgcgc gaaactcctg
     1441 aaggccgacg cgcccagcga ggtcgatccg gcgatcgtgc gcaggatcgc gatcgcgcag
     1501 gcgctctatg cgttcggcgc cgcgctgtgc gtcttcaata cctacgtcag cagcggtttc
     1561 attattctcg tacagctgaa ctacgtgctg gcgccaaaga tcggtgcgct ccggcggcta
     1621 tgaatccggc cggccgccgg caggctcgga ggcggcgtcg gcatcgacgc cgtgcggaat
     1681 caggtagtac gccgcgatcg ccaagaacgc cgcgaagctg accaccggga cgaccagcgc
     1741 gagcaccgtt gcggcgacgt agccgaccca gcccgtgcgg taggcacgca cggttcgggc
     1801 gatcgacgtc gcttcgaccg ccgggtcgaa cgccctcgcg cccacgagat gcccgagcag
     1861 cagattgtac atcgtcgagc agaacgacag cgtcacgccg tagagaaacg tggccgcggg
     1921 cagcgtggga tgcgcgccga gcgcgttggt ggcgaacggg atgaacgccg ttccgcccag
     1981 caggagcagg ttcaagaaga cggtggtgcg gtcgatcgcg cggacgagcc ggaagagcgc
     2041 gtgatggttt tgccacatga tgccgatcac gccgaagctg agggcgtagg ccagcagatt
     2101 cggccacatg cgcagcagcg ccgacgcgag ctcgcgatta gagttggggt cgtgcagctc
     2161 gggaaggacg aagccgagca ccaacagcgt gatcgcaaac gcgaagacgc cgtcggagaa
     2221 ggcctcgaac cgggacttgt tcatagcgac cgttcgcagc gcatgcggcg acatacttcg
     2281 gcaatcgcgg ggccgccttc cgaacatcgg tgcgccatac gctcggcgcc atgttcgacg
     2341 tgggagaatc gctgcgcggc gccctcgaaa cggcgcgcga acggctgaac gacgcgcagc
     2401 gcgccgtcgc gcgcgccaac gccggcggcg acgccggacg cagcgccggc gcggcgatgg
     2461 ctgcgacggc gcaggcggcg atcttcgccg aagcgctgct gggtgcggag cgcgcccgtt
     2521 tacaggagat caaggcggtg acgcgatgat cgagctgctg gacgccgctg ccagcgcgat
     2581 ggacgcgcag cgggcggcgc tcgacgtcgc ggcccgcaac gtcgccgcgg ccgaagccgc
     2641 cggccccgac ggagcctacg cgcgcgaagt tcccgagttt accgtgattg ccggagccgg
     2701 tggtgaagcc gatcgcgtcg cctttgccgg ttcgcatctg gaacggggca cgaacgtcga
     2761 cattctcacc gagatgatcg ccgtgatgaa cgcctcgcgg gcctacgaat cgaacgcgtc
     2821 gctgttcgac gtcggcaaaa cgctggctga aaaaacgatc gagctcggac ggct
//
LOCUS       DASUCJ010000337         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1211120, whole genome shotgun sequence.
ACCESSION   DASUCJ010000337 DASUCJ010000000
VERSION     DASUCJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211120"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="VFU90_04055"
     CDS             <1..>1096
                     /locus_tag="VFU90_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU5478978.1"
                     /translation="PPLQLQTRAENFSLSALNARGEVLIAFLGDVLRDQSVVVTERSA
                     NRLAGHILRGEAPVDEDQRTRRATVMSLVRDLVAAFGSSEDALLGLFGAFAYDLVFQI
                     EDLVQKRAREDDQRDIVLFVPDRLLAYDRATARGVVLSYDFAWNGKSSGGLARETSES
                     VYARTPRQAFSDHAPGEYQVTVETARASFARGDLFEAVPGQLFGEPCERSPAEVFQRL
                     CRINPSPYGALMNLGDGEFLVSASPEMFVRSDGRRIETCPISGTIARGVDAIGDAEQI
                     RQLLNSQKDEFELNMCTDVDRNDKARICVPGTIKVLARRQIETYSKLFHTVDHVEGML
                     RPGFDSLDAFLTHAWAVTVTGAPKLWAMQFV"
BASE COUNT          182 a          340 c          355 g          219 t
ORIGIN      
        1 tccgccgctg caattgcaga cgagggccga gaatttttcg ttgtccgcgc tgaatgcgcg
       61 cggcgaagtc ctgatcgcct ttctgggtga cgtgcttcgc gatcaaagcg tcgtcgtcac
      121 cgagcgcagc gcaaatcgcc tcgccggcca tatcctgcgg ggcgaggcgc ccgttgacga
      181 agatcagcgc acccgccgcg ccactgtcat gtcgctggtg cgcgatctcg ttgcggcgtt
      241 cggctcgagc gaagatgcgc tgctcggcct gttcggcgcc tttgcctacg atctggtgtt
      301 ccagatcgag gacctggtgc agaagcgcgc ccgcgaagac gaccagcgcg acatcgtgtt
      361 gttcgtgccc gaccggcttc tcgcctatga tcgcgccact gcgcgcggcg tggtcttgag
      421 ctatgacttt gcctggaacg ggaagtcgtc cggcgggttg gcacgcgaga cctccgagag
      481 cgtctacgcg cggacaccac ggcaggcctt ttccgaccat gcgccaggtg agtatcaggt
      541 cacggtggag acggcgcgtg catcgtttgc gcgtggtgat ttgttcgagg ccgtgccggg
      601 gcaactgttt ggcgaaccct gcgagcgttc gccggccgaa gtgttccagc ggctgtgccg
      661 gatcaacccg tcgccctacg gcgcgctgat gaatctcggc gacggcgagt ttctggtgtc
      721 ggcatcgccg gagatgttcg tccgttccga cggccgtcgg atcgagacgt gtccgatttc
      781 cggcaccatt gcccgtggcg tcgacgcgat cggcgatgct gaacagatcc ggcagctcct
      841 gaattcgcag aaagacgaat tcgaactcaa catgtgcacc gacgtcgatc gcaacgacaa
      901 ggcgcggatc tgcgtgccgg gcacgatcaa ggtgctggcc cgtcgtcaga tcgaaaccta
      961 ttcaaaactg tttcacaccg tcgaccacgt cgaaggcatg ctgcgaccgg gcttcgattc
     1021 gctcgacgca ttcctgaccc atgcctgggc cgtcacggta acgggcgcgc cgaaattgtg
     1081 ggcgatgcag ttcgtc
//
LOCUS       DASUCJ010000338         1226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_141425, whole genome shotgun sequence.
ACCESSION   DASUCJ010000338 DASUCJ010000000
VERSION     DASUCJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141425"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFU90_04060"
     CDS             complement(<1..807)
                     /locus_tag="VFU90_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478979.1"
                     /translation="MRAPTFVSVFALLLIGCGDDASRTGEPGDSSASDVGSEAGNPID
                     AGAFDATAPRDATHADAPSSPSSNADASGIADATGNVDSRAVDAMSAADASAVNGPDG
                     NGEDAGPCANGSWDGGFCCGAANTPTPEGPDDCGRCGHRCGGGACSSGVCQPFVVATL
                     PDTFGPYDIAANDSLVFVSGMNSNEDASVIYSVPLDGGGAHVLTHASDPPVQLFADPS
                     TLYWAGIQSISSDSIDGGGVTTIAARDTSSIAGFGADPSGAFLYWGEVSNT"
     gene            complement(972..>1226)
                     /locus_tag="VFU90_04065"
     CDS             complement(972..>1226)
                     /locus_tag="VFU90_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478980.1"
                     /translation="GYAVYLKAAASSAGGANWYWYEQLPPATAQSFGLEYTSTGVVAD
                     GFGGAGQPQTVCVGCHGAAGSDAPHTPSAGGGDEVYTPVH"
BASE COUNT          195 a          397 c          440 g          194 t
ORIGIN      
        1 ggtgttcgag acttcgcccc aatagaggaa cgcgccggat ggatcggcgc cgaagccggc
       61 gatggacgag gtatcgcgcg ccgcgatggt cgtgacgccg ccgccgtcga tcgaatccga
      121 agaaatcgat tggatgcccg cccagtagag cgtggaagga tcggcgaaga gctgcaccgg
      181 cggatccgac gcatgcgtca ggacgtgcgc tccgccgcca tcgaggggca ccgagtagat
      241 gacggaggcg tcttcgttcg agttcatgcc gctcacgaag acgaggctgt cgttcgcggc
      301 aatgtcgtag ggcccgaacg tgtccggcag agtggcgacc acgaagggct ggcacacgcc
      361 ggagctgcat gccccgcctc cgcaacgatg cccgcaacga ccgcagtcgt ccggtccttc
      421 gggggtcggg gtgttcgcgg ctccgcaaca aaagccgccg tcccagcttc cgtttgcaca
      481 cgggccggcg tcctcgccat tgccgtcggg cccattcact gcgcttgcgt cggcggcgga
      541 catcgcgtcg acggcgcgcg agtcgacgtt tccggtggcg tcggcgatcc cggacgcatc
      601 ggcgttcgaa gaagggctgc tgggtgcatc cgcgtgggtc gcgtctcgcg gcgcggtggc
      661 gtcgaaggcg ccggcgtcga taggattgcc ggcttcggag ccgacatcgg atgcgctgga
      721 gtcccccggt tcaccagtcc tgctcgcgtc gtcgccgcaa ccgatgagca agagtgcgaa
      781 gaccgagacg aaagtcggcg cgcgcatacc ggcatcgtcg ggggctcggc gccgaatgtc
      841 gacggtggga attcgacgct cggatacgac agcggcgcac cgaagacgtt gacgacgacg
      901 ccggttgcgt ctgccttgat ggcggacggg cctttccgtc agtcgtcggt gcatcgcgac
      961 cgagcgcctc ctcagtggac cggggtataa acctcgtcgc cgccgccggc gctcggagtg
     1021 tgcggggcat cggagcccgc ggcgccgtgg cagcctacgc acaccgtttg cggctgcccc
     1081 gcgccgccga agccgtcggc gacgacgccg gtcgacgtgt actcgagtcc aaatgattgg
     1141 gcggtcgcgg gaggcagctg ctcataccag taccagtttg ctccgccggc gctggaagcg
     1201 gccgccttga ggtagacggc gtaccc
//
LOCUS       DASUCJ010000339         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1333287, whole genome shotgun sequence.
ACCESSION   DASUCJ010000339 DASUCJ010000000
VERSION     DASUCJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333287"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFU90_04070"
     CDS             <1..618
                     /locus_tag="VFU90_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5478981.1"
                     /translation="RLLERAAKLSDELGGGSMTALPVIETQAGDFSAYIPTNVISITD
                     GQIYLTPQLFFQGIRPAVDVGLSVSRVGGAAQTKAMKSVAGQLKLELAQYRDLAAFSK
                     LASDLDKATQNQLMRGEKMTELLKQPQYQPQPMEDQVAIIYAATNGYVNEIATSRLQN
                     WARGFIDFVHAKHPDIPQAIAASGQLSDDVKKQLDAAIAEFNQSF"
     gene            620..1489
                     /gene="atpG"
                     /locus_tag="VFU90_04075"
     CDS             620..1489
                     /gene="atpG"
                     /locus_tag="VFU90_04075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5478982.1"
                     /translation="MPSVKDLRDRIRSLKNTQQITKAMKQVAGAKIRRAENARKQARP
                     YADTIAEMLRDLTQAVAHVDHPFMRPGKSGAPAGILLMTADKGLAGAFNSNVIRMAES
                     YARERGGALFYTVGLKGRNAVRRMGITDRPSWPLGGTSKLDTAREVARRAAADFDRGE
                     ISELVLVSSKLLSMLSQKPETRRLIPIEGAAAGDGIGDDKPRGAVEFAPSPEFVLSRL
                     LPKYLEFSIYSAMLETDAAFFAAQLVAMSNATDNASKLIDELTIAMNNARQAAITKEL
                     LEIVAGAEALGVA"
     gene            1521..3002
                     /gene="atpD"
                     /locus_tag="VFU90_04080"
     CDS             1521..3002
                     /gene="atpD"
                     /locus_tag="VFU90_04080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5478983.1"
                     /translation="MAAATGKVVQVLGNVVDVEFAPETLPKISDALLVRVNDDAHASS
                     NGAASPAVAGIDLGGSAMAPRELTLEVQDELGNNQVRCLAMGSTDGLVRGAPVTSVGG
                     PILVPVGEGTLGRIFNVLGKTIDSDEPVKAAAMWPIHRPAPEFKFQDPTPRIFETGIK
                     VIDLMAPYTRGGKVGLFGGAGVGKTVLIQELIRNIAYVHKGFSVFTGVGERTREGNDL
                     WIEMRESGVLAQTTLVFGQMDEPPGVRFRVAQTGVTMAEYFRDELGADVLLFMDNIFR
                     YLQAGSEVSALMGRMPSAVGYQPTLGTDMGVLEERITSTRKGSITSVQAVYVPADDYT
                     DPAVAVTFAHLDATTALSRPISELGIYPAVDPLASTSRILDPQIIGEEHYAVARGVQE
                     ILQRYKDLQDIIAILGVEELSEEDKVIVGRARRIQRFFSQPFFVAEQFTGRAGKYVKL
                     SETIASFKDILEGKVDNLPEAAFFYAGTIDEVKEAAAKMEAAA"
     gene            3008..3427
                     /gene="atpC"
                     /locus_tag="VFU90_04085"
     CDS             3008..3427
                     /gene="atpC"
                     /locus_tag="VFU90_04085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEU5478984.1"
                     /translation="MSGTIPFKLITPDRVAFEGDAELVIAVTTEGEEGILPRHAPFLA
                     ALKPGITRANVVREGAPSRLELATGEGFMQALPDRVTILVDEALRFEDVDVARSREEL
                     AAALERQKAAAGDPQGFAREQAVVDFASAKLRLTGHH"
     gene            3494..4846
                     /gene="murA"
                     /locus_tag="VFU90_04090"
     CDS             3494..4846
                     /gene="murA"
                     /locus_tag="VFU90_04090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5478985.1"
                     /translation="MLDRLETTLRVRGGARLEGSVATHGAKNAALPIMAAALLAKGTV
                     TLRRVPRITDVSVMWSLLEALGARIRYEGDDSVTIDASNVASYRAPYALVRKLAASFD
                     VVGPLLGRFGRAEVPLPGGCVLGTRATDMHEQAFVALGCEVHNAHGYLIAESRHRRLQ
                     SAEIEFRLPSVGATKNAMLAAVTAEGTTTLKNAAMEPEVADLAGFLVAMGAKIAGQGT
                     DTIVIEGVAELHGAEYEIIPDRIVTGTLLLAGAVTRGDVTVTACRPEHLRSALDKFVE
                     CGAAITTGKDWIRLQAGAIAGGTDILTAPYPGFPTDLQPQAVAFLATCPGTSVVEESI
                     FNARFSYVNELARMGADVKVAMESNAAVIKGVALLSGAPVEAPDIRAGAGLVVAGLAA
                     AGETEIIGLEYIDRGYERLEELLSDLGGQVQRSSGVTPLVEPAGFFETSEYPRVSAAG
                     "
     gene            4882..>5792
                     /locus_tag="VFU90_04095"
     CDS             4882..>5792
                     /locus_tag="VFU90_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5478986.1"
                     /translation="MDIGIDLGTANVLVHVKGRGIVLREPSVVAKDMNNGRVLAVGEE
                     ARLMLGKTPAHIQAIRPLRDGVIADFEVTEAMLSYFIKKVMKDRSWWSSIFKPKPHVT
                     ICVPAEITSVEERAVKDAAKLAGARDVDIVEEPMAAAIGAGLPIDGPSGSMVVDIGGG
                     TTDVAVISLGGIVVSQSIRVAGNKMDDAIVRYIRRVYNLMIGERTAEEIKIKLGSAYK
                     LEQELAMEIRGRDLINGLPKTVKITSEEIREALSEPVGAIVEAVKAVLEKTPPELAAD
                     VIDRGIVLTGGGALLRHMDELLTQETGV"
BASE COUNT         1084 a         1885 c         1886 g          937 t
ORIGIN      
        1 cggctgctcg aacgcgccgc gaaactctcc gacgagctcg gcggcgggtc gatgacggcg
       61 ctgccggtga tcgagacgca ggccggcgac ttctcggcgt acattccgac caacgtcatc
      121 tcgattacgg acggtcaaat ctacctgacg ccgcagctgt tcttccaggg catccggccg
      181 gccgtcgacg tcggtctttc ggtgtcgcgc gtcggcggcg ccgcgcaaac caaagcgatg
      241 aaatcggtcg ccggccagct gaagctcgag ctcgcgcaat accgcgatct ggcggccttt
      301 tccaagctcg ccagcgatct ggacaaggcg acgcaaaacc agctgatgcg cggcgaaaaa
      361 atgaccgagc tgctcaaaca gccgcaatat cagccgcagc cgatggagga ccaggtcgcg
      421 atcatttacg ccgcgaccaa cggctacgtc aacgaaatcg cgacgtcgcg cctgcaaaac
      481 tgggcgcggg gattcatcga cttcgtccac gccaagcatc ccgatatccc gcaagccatc
      541 gcggccagcg gccagctctc cgacgacgtc aagaagcagc tcgacgccgc catcgcggaa
      601 ttcaaccaga gcttctagca tgccgtccgt taaagatctg cgcgaccgaa tccggtcgct
      661 gaagaacacg cagcagatca ccaaggcgat gaaacaggtc gccggggcga agatccggcg
      721 tgccgaaaac gcccgaaagc aggcgcggcc gtacgcggac acgatcgccg agatgctgcg
      781 cgacctcacg caagcggtcg cgcacgtcga ccatccgttc atgcggccgg gaaaatccgg
      841 cgcgccggcc ggcattttgc tgatgaccgc cgacaaaggg ctggcgggcg cgttcaactc
      901 caacgtcatc cggatggccg aaagctacgc gcgcgagcgc ggcggcgcgc tgttctacac
      961 ggtgggcctc aaagggcgca atgcggtacg ccggatgggc ataacggacc ggcccagctg
     1021 gccgctcggc ggcacttcaa aactcgacac cgcccgcgag gtcgcgcgcc gcgctgccgc
     1081 cgacttcgat cggggggaaa tttccgagct cgttctcgtt tcgtcgaagc tgctttcgat
     1141 gctgtcgcaa aagcccgaaa cgcgccgctt gatcccgatc gaaggcgcgg cggccggcga
     1201 cggcataggc gacgacaaac cgcggggcgc cgtcgagttc gcaccgtcgc ccgagttcgt
     1261 tctctcgcgc ctcctcccca agtatctcga gttttcgatt tactcggcga tgctggagac
     1321 cgacgcggca ttttttgccg cgcagctcgt cgcgatgagc aacgcaaccg acaacgcctc
     1381 gaagctcatc gacgagctca ccatcgcgat gaacaacgcg cggcaagccg cgatcactaa
     1441 ggaattgctc gaaatcgtcg ccggcgcgga agcgctcggc gtcgcgtaac accggaaatc
     1501 tcacaaagga tcgaaacgtc atggctgcag ccaccgggaa agtcgtccaa gtgctcggaa
     1561 acgtcgtcga cgtcgagttc gcgccggaaa ccctgcccaa aatcagcgac gcgctgctcg
     1621 tccgcgtgaa cgacgacgcg cacgcctcga gcaacggtgc cgcttccccc gccgtcgcgg
     1681 ggatcgacct cggaggctcg gcgatggcgc cgcgcgagct gacgctggaa gtccaagacg
     1741 agctcggcaa caaccaggtg cgctgcttgg cgatgggatc gaccgacggt ctcgtgcgcg
     1801 gcgcgccggt aaccagcgtc gggggaccga tcctcgttcc cgtcggggaa ggcacgctgg
     1861 gccgcatctt caacgtgctc ggcaaaacca tcgacagcga cgaaccggtg aaagccgcgg
     1921 cgatgtggcc gatccatcgt cccgctccgg agttcaagtt tcaggatccg acgccgcgca
     1981 tcttcgaaac cggcatcaag gtcatcgact tgatggcgcc ctacacccgc ggcggcaaag
     2041 tcgggctgtt cggcggcgcg ggcgtcggga agaccgtgct cattcaagag ctgattcgca
     2101 acatcgccta cgtccacaag ggcttttcgg tgttcaccgg cgtcggcgaa cggacgcgcg
     2161 aaggcaacga cctgtggatc gagatgcgcg aatcgggcgt gctcgcacaa accaccttgg
     2221 tgttcggtca gatggacgaa ccgccgggcg tacgcttccg cgtcgcgcaa acgggcgtca
     2281 ccatggcgga atatttccgc gacgagctcg gcgccgacgt gctgctcttc atggacaaca
     2341 ttttccggta tctgcaggcc ggctccgagg tgtccgcgct gatggggcgc atgccgtcgg
     2401 cggtcggcta tcagccgacg ctcggcaccg atatgggcgt gctcgaggag cgcatcacct
     2461 cgactcgcaa aggctcgatc acgtcggtgc aagcggtcta cgttcccgcc gacgattaca
     2521 ccgaccccgc ggtagcggta acgttcgcgc atctcgacgc gacgaccgcg ctgtcgcgcc
     2581 cgatttcgga gctgggcatc tatcccgcgg tcgacccgct ggcctcgacg tcgcgcattc
     2641 tcgatccgca gatcatcggc gaagaacatt acgcggtcgc gcgcggcgtg caagagatcc
     2701 tgcagcgata caaagacctc caggacatca tcgcgatcct cggcgtggaa gagttgtcgg
     2761 aagaagacaa agtcatcgtg gggcgcgcgc gccgcattca acggttcttc tcgcagccgt
     2821 tcttcgttgc cgagcagttc accggacgcg ccggcaagta cgtcaagctt tccgagacga
     2881 tcgcttcctt caaggacatc ctcgagggca aggtcgacaa cttgcccgag gcggcgttct
     2941 tctatgccgg gacgatcgac gaagtcaagg aagccgccgc caagatggaa gcggcggcgt
     3001 aagcgacgtg tccggaacga ttccgttcaa gctgatcacg ccggaccgcg tcgccttcga
     3061 aggcgatgcg gagctggtca ttgcggtaac gaccgagggc gaggagggca tccttccgcg
     3121 gcacgcgccg tttctcgcgg cgctgaagcc ggggatcacg cgcgccaacg tcgtgcgtga
     3181 gggcgcgccg tcgcggctcg agctggcgac cggcgaaggc ttcatgcagg cgctgccgga
     3241 tcgcgttacg attctggtcg acgaggcctt gcgtttcgag gacgtcgacg tcgcacgcag
     3301 ccgcgaggaa ctcgcggcgg cgctcgagcg gcagaaggcc gccgccggcg atccccaagg
     3361 cttcgcgcgc gaacaggcag tcgtcgactt tgcgagcgcc aaactccggc tcaccggaca
     3421 ccactagcag ctgcggtttc tcgaggccac aatgttccgc cgcctcgcct agcgaagcgg
     3481 tggcggcagt aggatgctgg atcggttaga gacgacgctc cgcgttcggg gcggtgcgcg
     3541 gctggagggc tcggtggcaa cgcacggcgc gaagaacgcc gcgcttccga tcatggctgc
     3601 cgccctgctg gccaagggaa cggtgacgct gcgccgcgtc ccgcgcatta ccgacgtctc
     3661 cgtgatgtgg tcgctgctcg aggcgctcgg cgcccgcatt cgctacgaag gcgacgacag
     3721 cgtaacgatc gacgcgagca acgtcgcctc gtatcgcgca ccctacgcgc tggtgcgcaa
     3781 gctggccgct tcgttcgacg tcgtcggccc gctgctcggc cgattcggtc gtgccgaggt
     3841 tccgctgccc ggcggctgcg tgctcggcac gcgcgcgacc gacatgcacg aacaggcttt
     3901 cgtcgcgctc ggctgcgagg tgcacaacgc gcacggctat ctcatcgccg agtcgcgcca
     3961 ccggcgcctg cagtcggccg aaattgaatt tcgcttaccg agcgtgggcg cgacgaagaa
     4021 cgccatgctc gcggccgtga cggccgaagg cactaccacg ctgaagaacg ctgcgatgga
     4081 acccgaagtc gccgatctgg ccggctttct cgtcgcgatg ggtgcgaaga ttgccggaca
     4141 agggaccgac acgatcgtca tcgagggcgt cgcggaactg cacggcgcgg aatacgaaat
     4201 catccccgac cggatcgtga ccgggacgct gctgctcgcc ggcgcggtga ctcgcggcga
     4261 cgtcaccgtt accgcctgcc gtcccgaaca tctgcgcagc gcgctcgaca agttcgtcga
     4321 atgcggcgcc gccatcacga ccggcaagga ttggattcgt ttgcaagccg gggcgatcgc
     4381 cggcggaacc gacatcctca ccgctccgta ccccggcttc ccgaccgatc tgcagccgca
     4441 ggccgtcgcg tttctcgcga cgtgtccggg aaccagcgtc gtcgaagagt cgatcttcaa
     4501 cgcgcgcttt tcctacgtaa acgagctggc gcgcatgggt gccgacgtga aggttgcgat
     4561 ggaaagcaac gccgcggtga tcaaaggcgt cgcgctcctc tcgggtgccc cggtcgaggc
     4621 acccgacatc cgggcgggtg ccggcttggt ggtcgccggt ctcgcggcgg ccggcgaaac
     4681 ggaaatcatc ggtctcgaat acatcgatcg cgggtacgag cggctcgagg agctgctttc
     4741 ggatttgggc gggcaggtgc agcgcagcag cggcgtcacg ccgctcgtcg agccggccgg
     4801 gtttttcgaa acgagcgaat atccgcgagt ttcggcggcc ggatagtcaa tcgaatcgtt
     4861 ttttccaact aggagcgtgt tttggatatc gggatagatc tcggcacggc caacgtgctc
     4921 gtccacgtca agggcagagg catcgtactg cgcgagcctt ccgtcgtggc caaggacatg
     4981 aacaacggca gggtgctggc cgtcggcgag gaggcccgct tgatgctggg caaaaccccg
     5041 gcgcacattc aagccattcg tccgctgcgc gacggcgtca tcgccgattt cgaagtcacc
     5101 gaagcgatgc tctcgtattt catcaagaag gtcatgaaag accgttcctg gtggtcgtcg
     5161 atcttcaagc cgaagccgca cgtgacgatt tgcgttccgg cggagatcac cagcgtcgag
     5221 gaacgcgccg tcaaggacgc cgccaagctc gcgggcgcgc gcgacgtcga catcgtcgag
     5281 gagccgatgg cggcggcgat cggcgcgggc ttgccgatcg acggaccgtc ggggagcatg
     5341 gtcgtcgaca tcggcggcgg cacgaccgac gtcgccgtca tctcgctggg aggcatcgtc
     5401 gtttcgcagt cgatccgcgt cgccggcaat aagatggacg acgcgatcgt gcgctacatt
     5461 cggcgcgtct acaacctcat gatcggcgaa cgcacggccg aggagatcaa gatcaaactc
     5521 ggctcggcct acaaactcga acaggagctg gcgatggaga ttcgcggccg cgacttgatc
     5581 aacggcctgc cgaagaccgt caagatcacc agcgaagaga ttcgcgaggc gctctccgaa
     5641 ccggtcggcg cgatcgtcga agcggtcaag gccgtgctcg agaagacgcc gccggagctg
     5701 gccgccgacg tcatcgatcg cggcatcgtc ctcaccgggg gcggtgcgct cctgcgccac
     5761 atggacgagc tgctcacgca ggagacaggc gt
//
LOCUS       DASUCJ010000340         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1104078, whole genome shotgun sequence.
ACCESSION   DASUCJ010000340 DASUCJ010000000
VERSION     DASUCJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104078"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..>1274)
                     /locus_tag="VFU90_04100"
     CDS             complement(95..>1274)
                     /locus_tag="VFU90_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5478987.1"
                     /translation="ALVALLTLALTVPTGGRALAQAKIINDPSIFEPVFASDPTGAIR
                     AARERIAAGDLADAVKGLEIYVVSHPGEAGPERFLGDLYYRQGRFDKAEEVYKNILTH
                     DPGDRETHNRLGVVYATENRVDDAIVQFDKALPGTDSVADLVAMHLRKGDLQQYRQQM
                     ERLAAAQPTDADIQAEMGQVYAALHRPADALGYFMRALDSDPQSLTALNGLGLAYFDL
                     HNFSEAEKQFETCLALDNTNYTCTNNLGAAYLESGDDDACKPYLLRARSLAPERPEAL
                     VNFGFLADRHGDWKQAVDFYVRATAVGPYLPEAYIDLGIDYEHNGLYQLAQAALLKGI
                     AAAPEDGRIRYLLAVAYNAQGEKSLALEQLKVAEQSLDPDVARIATEESAKLSAAPHG
                     "
BASE COUNT          197 a          391 c          447 g          239 t
ORIGIN      
        1 gcacgatggt ggcgtgctcg caagcgaggc gtgcgaccac cctaccgtcg ggctgcggcg
       61 ggtcccggaa aggaagcgtt agcgcgggcg aggcttaccc gtggggcgcg gcgctcagtt
      121 tcgcggattc ttcggtagcg atgcgcgcga cgtcgggatc gagcgactgc tcggcgacct
      181 tcagctgctc gagcgcgagc gatttttcgc cctgcgcgtt gtacgcgacc gcgagcagat
      241 agcgaatccg cccgtcttcg ggcgcggccg cgatgccttt gagcagcgcc gcctgcgcga
      301 gctggtagag gccgttgtgc tcgtagtcga tgcccagatc gatgtaggct tcggggagat
      361 acgggccgac cgccgtggcg cgtacgtaga agtcgacggc ctgtttccaa tcgccgtgcc
      421 ggtcggccag gaatccgaag ttcaccagcg cttcggggcg ctcgggcgcg agcgagcggg
      481 ctcgcagcag atacggcttg caggcgtcgt cgtcacccga ctccaggtac gccgcgccga
      541 ggttgttcgt gcacgtatag ttcgtgttgt cgagcgcgag acaggtttcg aactgctttt
      601 ccgcttcgct gaagttgtgc aagtcgaagt acgccagccc cagtccgttg agcgccgtca
      661 acgactgcgg atcgctgtcg agcgcccgca tgaagtagcc gagggcgtcg gccggacggt
      721 gcagcgctgc gtacacttgt cccatttcgg cctgaatgtc ggcgtcggtc ggttgggccg
      781 ccgccagccg ttccatctgc tggcgatact gctgcaggtc gcccttgcgc aaatgcatcg
      841 ccacgagatc ggctaccgaa tcggtgccgg ggagtgcctt gtcgaactgc acgatcgcgt
      901 cgtccacgcg attctcggta gcgtagacca cgccgaggcg attgtgcgtt tcgcggtcgc
      961 ccggatcgtg ggtgaggatg ttcttgtaca cctcttcggc tttgtcgaag cggccttgcc
     1021 ggtaatacaa gtcgccgagg aagcgttcgg gaccggcttc gccggggtgc gagacgacgt
     1081 agatctccaa acccttgacc gcgtccgcca gatcgccggc ggcgatccgt tcgcgagccg
     1141 cgcgaatcgc gccggtcgga tccgacgcga ataccggctc gaaaatcgac ggatcgttga
     1201 tgatcttggc ttgagcgagc gcgcggccgc cggtggggac ggtcaaggcc agcgtcagca
     1261 gcgcgacgag cgcg
//
LOCUS       DASUCJ010000341         1401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_508031, whole genome shotgun sequence.
ACCESSION   DASUCJ010000341 DASUCJ010000000
VERSION     DASUCJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508031"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1401)
                     /locus_tag="VFU90_04105"
     CDS             complement(<1..>1401)
                     /locus_tag="VFU90_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478988.1"
                     /translation="LSQIFQARSQRATAPTVYLFTDNQASGWREAASQGIERLVPDDA
                     QLVVVDVGSHEPLANRAVIGEPPRQHRAIVGLPLVLRPRVANDSETELAEVTVGVFVD
                     DAEIARVALTIKPGDSEQQEIVFTPAEPGVVRGRFEIDADRFPDDDQFLFTLSVSPPI
                     KVLLVNGNPAGDVYENEVLYLRTALTAHGEEQERPNEPAPAEVSAASEPASPLGPSKQ
                     YVRSLDVQEIQEGQVNPDTLRDASVVILANCGGLNQQHFLWLHDFVSAGGGLLIFPGD
                     RVNYDVYNKQFFAVPGPLKERLTPVEMGPPLGDPQKPETFVELSAVDFAHPALAVFDD
                     PDARYLTTAHFSRRFPLTFDEATPGAWALARFSPKQAALVESRFRDGAVLVAAFPMHA
                     KWTNLPLKPEFVPLVLRLVNHLEHRPELDAPSVVPADGAAEIAVAGDWSPLSGRVIDG
                     EQRATDLEFKRSGSRLV"
BASE COUNT          250 a          452 c          468 g          231 t
ORIGIN      
        1 gaccagccgc gaaccggaac gtttgaattc caagtcggtc gcgcgctgtt cgccgtcgat
       61 cacgcggccc gacaggggcg accaatcgcc cgccacggcg atctccgccg caccgtcggc
      121 cggcacgacc gagggcgcat cgagttccgg acgatgctcg agatgattga ccagccgcag
      181 caccagcggc acgaactcgg gcttcagcgg cagattcgtc catttggcgt gcatgggaaa
      241 ggccgctacg agcacggcgc cgtcgcgaaa gcggctttcg accagcgcgg cctgcttggg
      301 cgagaagcgg gccaaggccc aggcgcccgg cgtcgcttca tcgaaagtga gcggaaagcg
      361 gcgcgaaaag tgcgccgtgg tcagatagcg cgcgtcggga tcgtcgaaca ccgccaaggc
      421 cgggtgggcg aaatcgaccg cgctcagttc gacgaaggtt tccggcttct gcggatcgcc
      481 caacggcggc cccatctcga ctggcgtcaa acgctccttg agcggccccg gcacggcgaa
      541 gaactgtttg ttgtaaacat cgtaattcac gcgatcgccg ggaaagatca gcagtccgcc
      601 gcccgccgag acgaaatcgt gcagccagag gaagtgctgc tggttcagcc cgccgcaatt
      661 ggccaggatc acgacgctgg cgtcgcgcaa ggtatcgggg ttcacctgcc cttcctgaat
      721 ctcctgaacg tcgagcgagc gaacgtactg cttgctcggc cccagcggcg acgccggttc
      781 gctggcggcg ctgacttcag ccggcgccgg ctcgttcggc cgctcctgct cttcgccgtg
      841 tgcggtcaaa gcggtgcgca gatagaggac ttcgttctcg taaacgtcgc ccgccggatt
      901 gccgttgacc aacagcacct tgatcggcgg ggaaaccgaa agcgtaaaca ggaactggtc
      961 gtcgtccgga aagcggtcgg cgtcgatctc gaagcggccg cgaactacgc ccggttcggc
     1021 gggggtgaac acgatttcct gttgttccga gtcgccgggc ttgatcgtga gcgccacgcg
     1081 ggcgatttcg gcgtcgtcga cgaatacgcc gaccgtgact tcggccaact cggtttcgga
     1141 atcgttggcc acgcgcggcc gcagcacgag cggcaaaccg acaatcgcgc gatgctgccg
     1201 cggcggctcg ccgattaccg cccggttggc cagcggttcg tgcgagccga cgtcgactac
     1261 gaccaactgt gcgtcgtccg gcacgagccg ctcgatgcct tgcgaggccg cttcgcgcca
     1321 gccgctggcc tggttgtcgg tgaacaggta gacggttggc gcggtcgcac gctgcgagcg
     1381 agcttgaaaa atttgcgaca g
//
LOCUS       DASUCJ010000342         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1501505, whole genome shotgun sequence.
ACCESSION   DASUCJ010000342 DASUCJ010000000
VERSION     DASUCJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501505"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1085)
                     /locus_tag="VFU90_04110"
     CDS             complement(<1..>1085)
                     /locus_tag="VFU90_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5478989.1"
                     /translation="LDRLQHVDLGIKTANVLTFDLHLPDARYDSTARGLTYERVARTL
                     EALPGVRAAGGVSKLPATGQFNTWGARPLSGPLAGDQKHSVSDENRVVSGDYFRAVGI
                     PIVRGRAFDGRDVPGAPPAMIITQSLADKLFPGVDPIGQSLTTGGRNGTVVGIAGETA
                     VTNEGTPGMFVYHPHRQFAGDRNWALTQVVALKGPAALNGAILRSVRSAIASIDPLLV
                     VYHPTTLDEAVGRGAAQRVFTLRILGAFAVVALILAALGLFGVLSYGVRLRGREFGIR
                     MALGAQRSSIRVMVLRQGLIVSGLGIAIGVAFAAGLSRFMRAMLFEVSPLNPIVFGGA
                     VGFMIAVAALSAYLPAHRATAADPRRA"
BASE COUNT          206 a          379 c          356 g          144 t
ORIGIN      
        1 cgcgcgccga ggatccgcag cggtcgcccg atgcgctggg agatacgccg acagcgccgc
       61 gacggcgatc atgaatccca ccgcaccacc gaagacgatg gggttgaggg gactcacctc
      121 gaacaacatc gcgcgcatga accgcgaaag cccggcggcg aaggcgacgc cgatggcgat
      181 gccgaggcca ctcacgatca atccctgacg cagcaccatg acgcgaatgc tcgaccgctg
      241 cgcgccgagc gccatccgaa taccgaattc ccgtccgcgc aggcgaacgc catacgacag
      301 cacgccgaag agaccgagcg ccgcgagaat cagcgcgacg accgcgaacg ccccaagaat
      361 tcgcaacgtg aacacgcgct gagcggcgcc gcggccgacg gcttcgtcca gcgtcgtcgg
      421 gtgatacacg accagcagtg gatcgatcga cgcgatggcg gaacgcaccg atcggagaat
      481 cgcgccgttc aaggcggccg gtcccttcag cgccacgacc tgcgtgagcg cccagttgcg
      541 atcgcccgcg aactgtcggt gcgggtgata tacgaacatg ccgggcgtgc cctcgttggt
      601 gaccgccgtt tcgccagcga tgccgaccac cgtaccgttc cgaccgccag tcgtcaggct
      661 ctgtccaatc ggatcgacgc cgggaaacag cttgtcggcg agcgattgcg tgatgatcat
      721 ggccggcggc gcacccggga catcgcgacc gtcgaacgcg cggccgcgca cgatggggat
      781 gccgaccgcc cggaagtagt cgcccgacac cacccgattc tcatcgctca ccgagtgctt
      841 ctggtcgccc gccagcggtc cgctgagcgg acgagcgccc cacgtgttga attgtcccgt
      901 agccggcagc ttcgacacgc caccggccgc gcgcacaccg ggcagcgcct cgagcgttcg
      961 cgcgacccgc tcgtaggtga ggccgcgcgc ggtcgagtcg taacgagcgt cgggcaggtg
     1021 caggtcgaac gtcaagacgt tcgcggtctt gatgccgagg tcgacgtgct gcaggcgatc
     1081 cagac
//
LOCUS       DASUCJ010000343         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1226425, whole genome shotgun sequence.
ACCESSION   DASUCJ010000343 DASUCJ010000000
VERSION     DASUCJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226425"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1418
                     /gene="dnaE"
                     /locus_tag="VFU90_04115"
     CDS             24..>1418
                     /gene="dnaE"
                     /locus_tag="VFU90_04115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5478990.1"
                     /translation="MTVLCQNREGYLHLSQLISRSYTQGRHGDRALVDPAWFDGHGAG
                     LIALAGRESDIGRNLIAGKRDAALASTRAWRRHFDDRLYLELSRCGHADEAHFISAAL
                     QLAQDADCPVVATNDVRFGVREDFEAHEARVCIHQGRLLNDPKRPRDYTPEQYLKSAE
                     EMIELFADIPEALENSVEIAKRCNLEMKFGEYFLPAFPVPALDSEASFIRSQAHTGLE
                     VRLSKHGLAPDFTHADYTSRLERELDVIVKMGFAGYFLIVADFIGWAKKNGIPVGPGR
                     GSGAGSVVAWSLGITDLDPLRYGLLFERFLNPERVSMPDFDVDFCMDRRDEVIDYVAN
                     KYGREKVSQIITYGTMAAKAVLRDAGRVLGMGYGQVDQIAKLIPLKPADPLSLDDALG
                     ASERSRRETDRIVPEFKARYESEDEVRELVDLARRIEDLVRNAGKHAGGVVIAPSALT
                     DFAPLYCEGSGDGVV"
BASE COUNT          257 a          487 c          437 g          237 t
ORIGIN      
        1 ccagtgacgc gtcgccgtcg cgcctgaccg tgctatgcca gaaccgcgaa ggttacctgc
       61 acctttcgca actgatctcg cgcagctaca cgcaaggtcg gcatggcgac cgcgcgctcg
      121 tcgatccggc ctggttcgac gggcacggcg ccggactcat cgcactggcc ggacgcgaga
      181 gcgacatcgg ccggaatctc atcgccggca agcgcgacgc tgcgctggcc agcacccgcg
      241 cctggcggcg tcacttcgac gatcgcctgt atctggaatt gtcgcgctgc ggccacgccg
      301 acgaggcgca tttcatttcc gccgcgctgc agctcgcgca ggatgccgac tgtcccgtcg
      361 tcgcgaccaa cgacgtgcgt ttcggcgtgc gcgaggattt cgaagcgcac gaggcacgcg
      421 tgtgcatcca ccagggtcgc ttgctcaacg acccgaagcg cccgcgcgac tacacacccg
      481 agcagtacct gaaatccgcc gaggaaatga tcgagctgtt cgccgatatt cccgaagcgc
      541 tggaaaacag cgtcgagatt gccaagcgct gcaatctgga aatgaagttc ggcgaatact
      601 tcctgcccgc ctttccggtt ccggcgctcg actcggaagc ctcgttcatc cgcagccaag
      661 cgcacaccgg cctggaagtg cgtctgtcca agcatggact cgcccccgat ttcacgcacg
      721 cggactatac gagccggctc gagcgcgaac tcgacgtgat cgtgaaaatg ggcttcgccg
      781 gctacttcct gatcgtcgcc gacttcatcg gctgggcgaa aaagaacggc attcccgtcg
      841 ggccgggacg cggctccggc gccggctcgg tcgtcgcctg gagtctcggc atcaccgacc
      901 tcgatccgct gcgctatgga ttgctgttcg aacgcttcct caatcccgaa cgcgtgtcca
      961 tgcccgactt cgacgtcgac ttctgcatgg accgccgcga cgaagtgatc gactacgtcg
     1021 ccaacaagta cggccgcgag aaagtcagcc agatcatcac ctacggcacg atggcggcca
     1081 aggccgtgct gcgcgacgcc ggccgcgtgc tcgggatggg ctacggccag gtcgatcaaa
     1141 tcgccaagct catcccgctc aagccggccg atccgctcag cctcgacgat gcgctcggcg
     1201 ccagcgagcg ctcgcgccgg gaaaccgatc gcatagttcc cgaatttaag gcgcgctacg
     1261 aatccgagga cgaggtgcgc gaactggtgg atctggcgcg ccgcatcgag gacctcgtgc
     1321 gcaatgccgg caagcatgcc ggcggcgtgg tgatcgcacc gagcgcgctg accgatttcg
     1381 ccccgctcta ttgcgaaggc agcggcgacg gcgtcgtc
//
LOCUS       DASUCJ010000344         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_966516, whole genome shotgun sequence.
ACCESSION   DASUCJ010000344 DASUCJ010000000
VERSION     DASUCJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966516"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VFU90_04120"
     CDS             <1..1349
                     /locus_tag="VFU90_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HEU5478991.1"
                     /translation="AIVRWLAAESNDPRTASFVVSTDMLAYGGLIASRIPGASYADAY
                     SRLQELASIRARRPAAWIGAFGTVMRLAPTGTPAASGFFAAYPAWTYLQQYANLPDPP
                     PAGDAQPAQTLRDKIGAATLDAYLATRARNLAVDRHLLRLAAAGTIDRLALGQDDAGP
                     VGLHVREVALLQAEVAQLDAGDRVSIEPGADELGMALVANALARQAGWTPRIGVRYST
                     PDGARFQDPLEFAPIADAIDGLIGLCGGVRDDANPDIVLFVRVPNTPETDDDAFAASM
                     DRAIGAGTRAALADLSFLKSYRDQAAFAQRILQSGLAAKLDAYAAWNTNANTVGTALA
                     EAVAASAGRRTGTYDALAHRTFTFVRFVDDYAFHDEIRPSLNASLDAQGVTDHTMLPA
                     SVAVPLSERNRALLWNRAQSILNLLDPGYHIAAMQIELPWDRTFETAVDVGIAPNL"
     gene            1395..2027
                     /locus_tag="VFU90_04125"
     CDS             1395..2027
                     /locus_tag="VFU90_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU5478992.1"
                     /translation="MDARFIATAFATAITIIDPIGMIPLTLGATARVSTQRRNAIVDQ
                     AVLVAAAIIAFMGLVGRPLLDYLGITLPAFTIAGGILLFLIAIDMLFARPTGAKRTEA
                     EEREAAEGENPAVFPLAVPMIAGPGTIATVLLLVDLSHGDRANLGVVAAAYAGALLVT
                     WLCMRGSTLLLRVIGNTGIHVISRILGIILAALAVQFVINGLLQTPLLKH"
BASE COUNT          325 a          718 c          749 g          367 t
ORIGIN      
        1 acgcgatcgt tcgctggctc gccgccgagt cgaacgatcc gcggacggcg tcgttcgtgg
       61 tgtcgaccga catgctcgcg tacggcggcc tgatcgcgtc gcgcatcccg ggcgcgtcgt
      121 acgccgacgc ctactcccgg ctgcaggagc tggcttcgat tcgcgcgcgg cgtcccgcag
      181 cgtggatcgg cgcgttcgga accgtcatgc gcttggcgcc gaccgggacg cccgccgcga
      241 gcggattttt tgcggcgtat ccggcgtgga cgtacctgca gcaatacgcc aacctgccgg
      301 atccgccgcc ggccggcgac gcgcagccgg cgcagacgct gcgcgacaag atcggcgcag
      361 cgacgctcga tgcgtatctg gccacgcgcg cgcgcaactt ggcggtcgac cggcatttgc
      421 tgcggctcgc ggcggccggc acgatcgacc ggctggcgct cgggcaggac gacgcgggcc
      481 cggtcgggct gcacgtgcgc gaggtggcgc tgctgcaggc ggaggtcgcg cagctcgatg
      541 ccggcgaccg cgtttcgatc gaacccggcg ccgacgagct ggggatggcg ctggtcgcta
      601 acgcgctcgc gcggcaggcg ggctggacgc cgcggatcgg cgtgcgctat tcgacgccgg
      661 acggcgcgcg gtttcaggat ccgctggagt tcgcgccgat cgccgacgcg atcgacggat
      721 tgatcggctt gtgcggcggc gttcgcgacg atgccaaccc cgatatcgtg ctgttcgtgc
      781 gcgtgccgaa tacgccggag accgacgacg atgcgttcgc ggcttcgatg gatcgcgcga
      841 tcggcgccgg tacgcgggcg gcgctggccg atttgtcgtt tctcaaatcg taccgcgatc
      901 aagccgcgtt cgcgcagcgc atattgcaat cgggactggc ggcgaagctc gacgcgtacg
      961 cggcctggaa caccaacgcc aacaccgtcg gaacggcgct ggcggaagcg gtcgcggcga
     1021 gtgcagggcg gcgaacggga acctacgacg cgctcgcgca tcgcacgttt acgttcgtgc
     1081 gcttcgtcga cgactacgcg tttcacgacg aaatccgtcc gtcgctcaac gcatcgctcg
     1141 acgcgcaggg cgttacggac cacacgatgc tgccggcgag cgtcgccgtg ccgctgtccg
     1201 aacgcaaccg cgcgctgctc tggaaccggg cgcagagcat cttgaacctc ctcgacccgg
     1261 gctatcacat cgcggccatg cagatcgagc tgccctggga ccggacgttc gagaccgcgg
     1321 tcgacgtggg gatcgccccc aatctgtagc cggcggcgcg gttttcgatt gaccgcgctg
     1381 ttatgatgga gccgatggac gcccgcttca tcgcgaccgc cttcgccacg gcgataacga
     1441 tcatcgatcc gatcggcatg attccgctga cgctcggcgc gaccgcgcgc gtttcgacgc
     1501 agcggcgcaa cgcgatcgtc gatcaagcgg tgctggtggc ggccgcgatc atcgcgttca
     1561 tggggctggt cggacggccg ttgctcgact atctcggcat cacgctgccg gcctttacga
     1621 tcgcgggcgg catcttgctg tttctgatcg cgatcgacat gctgttcgcg cggccgaccg
     1681 gcgcgaaacg aacggaagcc gaagaacgcg aagccgccga aggcgaaaat ccggcggtat
     1741 ttccgctcgc agtgccgatg atcgccggtc ccggaacgat cgcgacggtg ttgctgctgg
     1801 tcgatctctc gcacggcgat cgcgccaacc tcggcgtggt ggcagccgcc tacgccgggg
     1861 cgctgctggt cacgtggctg tgcatgcgcg gctcgacgct gttgctgcgc gtgatcggca
     1921 ataccggcat ccacgtcatc agccgcatcc tcggcatcat cctcgccgcg ctggccgtgc
     1981 agttcgtgat caacggcttg ctgcagacgc cgttactgaa gcactaaatc cgctcgtttt
     2041 gcggtaaggc ttccggacac aaccgtgagg gtggggatgc cgtcgctcgg acgttcgtcg
     2101 ctcgctaaca caacggtgtc atcctgagcg gagctcgcgt gagcgagcgc agtcgaagg
//
LOCUS       DASUCJ010000345         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_202509, whole genome shotgun sequence.
ACCESSION   DASUCJ010000345 DASUCJ010000000
VERSION     DASUCJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202509"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFU90_04130"
     CDS             complement(<1..768)
                     /locus_tag="VFU90_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5478993.1"
                     /translation="MFSSSGRKVVAAWIVLSAIATPIVAIVVGPHLPPGNGSAQATEQ
                     VFDNTWMTAIVTPIICLLVVFFAYVLVVNRAPKGQELRDGPPLRNDATVQTVWIALTS
                     ITVLFLAGFGTYELLQNGSGGGQGPSPIAVPGGTKIPVQVIAQQWQFTYRYPTFGGLE
                     TPQLVLPADSYIELHVTSLDAIHSFWAYELGVKADANPGVDNVAYVKTKGPRTFHVRC
                     AELCGLWHGYMFDNGRVVPQAQFTAWIKSQQHDFAAVQ"
     gene            complement(758..>1217)
                     /locus_tag="VFU90_04135"
     CDS             complement(758..>1217)
                     /locus_tag="VFU90_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478994.1"
                     /translation="LVRDTHGKWLRHTVWRLPANATVHVTIYNFDGAGGLRNPLYARP
                     QGIDGNSFQLDGKATDVINPNDTSHTFAVPAYGLVVPLTGVADNAKNQCGYAPCAMSM
                     AHHTITFTFHTGKAGHYRWQCFVPCAAGWIDGFGGPMQTIGYMDGFLDVL"
BASE COUNT          196 a          400 c          422 g          199 t
ORIGIN      
        1 ctgcaccgct gcgaagtcat gctgctgcga cttgatccac gccgtgaact gcgcctgcgg
       61 cacgaccctg ccgttgtcga acatgtagcc gtgccagagc ccgcacagct cggcgcaccg
      121 aacgtggaac gtgcgcggcc ccttcgtctt cacgtacgcg acgttgtcga cgcccgggtt
      181 cgcgtccgcc ttgaccccga gctcgtacgc ccagaacgaa tggatcgcgt cgagcgaagt
      241 gacgtgcagc tcgatgtacg agtcggccgg gaggacgagc tgcggcgtct cgagcccgcc
      301 gaacgtcgga tagcggtacg tgaactgcca ctgctgggcg atgacctgaa cggggatctt
      361 cgttccgccc gggacggcga tcgggctcgg gccctggccg ccgccggagc cgttctgcag
      421 gagctcgtac gtcccgaacc cggcgaggaa gagaacggtg atcgaggtga gcgcgatcca
      481 gaccgtctgg acggtggcgt cgttgcgcaa cggcgggccg tcgcgaagct cctgaccctt
      541 cggcgcgcgg ttcaccacga gcacgtacgc gaagaacacg acgaggaggc agatgatcgg
      601 cgtgacgatc gccgtcatcc aggtgttgtc gaagacctgc tcggtcgcct gcgcgctgcc
      661 gttcccgggc gggaggtgcg ggccgacgac gatcgcgacg atcggcgtcg cgatcgcgct
      721 gagcacgatc cacgccgcga cgaccttgcg gccgctgcta gagaacatcg aggaagccgt
      781 ccatgtagcc gatcgtctgc atcggtccgc cgaagccgtc gatccagccg gcggcgcacg
      841 gcacgaagca ctgccagcgg tagtggccgg cctttccggt gtggaacgtg aacgtgatcg
      901 tgtggtgcgc catcgacatc gcgcacggcg cgtagccgca ctgattcttc gcgttgtcgg
      961 ccacgccggt gagcggcacg acgagcccgt acgccggcac cgcgaacgtg tgcgacgtgt
     1021 cgttcgggtt gatgacgtcc gtcgccttgc cgtcgagttg gaacgagttc ccgtcgatgc
     1081 cctgcggtcg cgcatacagc ggattgcgca gaccgcccgc gccgtcgaag ttgtagatcg
     1141 tgacgtgcac ggtggcgttc gccggcagcc gccagaccgt gtggcgcagc cacttcccgt
     1201 gcgtgtcgcg cacgaga
//
LOCUS       DASUCJ010000346         9543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1195866, whole genome shotgun sequence.
ACCESSION   DASUCJ010000346 DASUCJ010000000
VERSION     DASUCJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..481
                     /locus_tag="VFU90_04140"
     CDS             158..481
                     /locus_tag="VFU90_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478995.1"
                     /translation="MTLVIRVKVPLDLHSVAADGSGAFALATPLRVRELLALAVREAI
                     GSRAPEDKFARSVHRTLAGLASGDFTLVIDGRTFADPDAVVVCDGTADVRFFIASRRR
                     AGVRR"
     gene            564..1190
                     /locus_tag="VFU90_04145"
     CDS             564..1190
                     /locus_tag="VFU90_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478996.1"
                     /translation="MAVAMGVQALVAVVWRIPHGADLDGAIVLPILTTLVYGFVAADA
                     RPEPAAASSVWERVLERSWAVIVIDFVVSYVTALGLAGSTSPNALDLIAGLSAFVLSV
                     LFVFADASATVDGDVGVLTVIPYGFVRSVQAAWRRPVLPRALAIFSIQLLVAAVQIAL
                     GALLQRGHVADALFWSEIPLLTVVVPPLSALTVLVYLDARAAPAQSAE"
     gene            1311..2075
                     /locus_tag="VFU90_04150"
     CDS             1311..2075
                     /locus_tag="VFU90_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478997.1"
                     /translation="MSATTLGAAADQKYDVHGEDVYRVGSSPSASRVAYTGEQRLLVE
                     RDGASTRFDAQASYTRDADGVKRRIDARFVQELLPDGAFEDRIDDDPDFLTILNQPFA
                     IRLDGATLRDLQNLRAPVPFDASSPLGGRDVLRGYLRPAPPGKICGRPVVAVAFEAEG
                     PMTAPMPTRTSATMSGRMRMQGTAYYARSDAMLLALDATLTIVAQLHEGTSVTPVRVV
                     YRRFIRAALPSPAPAAKTPPPTPLPTGGGTAPPPAR"
     gene            complement(2011..2403)
                     /locus_tag="VFU90_04155"
     CDS             complement(2011..2403)
                     /locus_tag="VFU90_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5478998.1"
                     /translation="MRQTIEPTEISFDAYTMCSFLPNLDSARAIPLALIGYRQNEDPW
                     FVWLVPKDGIPNEGLTDDPYYAKVLRDPRAYFKKKLEKYVEETGCVKRGIEMLVDCHQ
                     NHLAFTPLTELGEVPFRHPLEVELVGAF"
     gene            2721..3353
                     /locus_tag="VFU90_04160"
     CDS             2721..3353
                     /locus_tag="VFU90_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5478999.1"
                     /translation="MPSAVPTGKIAAARPAATAVPGIETFERVVDDYQRRLYGFALRM
                     TGNREDAEEIVQDAFVRAYRALGKMSPEQRAELRLQPWLYTITLNVTRNRLRSKRPSN
                     VALDALADPDALLRESNEGPPQPETIVERNTDVALVERALMQLPMHLRAAATLRFIEG
                     RSHPEIAEILHQPIGTVKSHVHRAVRILRRILGPQVGRFTPEGAPTNALS"
     gene            3340..4071
                     /locus_tag="VFU90_04165"
     CDS             3340..4071
                     /locus_tag="VFU90_04165"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU5479000.1"
                     /translation="MRCRDVEIMWDEIRGDCKESLKETVETHLLACPPCQELYEEFEG
                     VAYCLSCLPQPEPSCNLTKKVVEHIASLKLKDRTPILLCSVETPIGRAYVGFKERRIA
                     YVGIDTGDPLEEVRGRVERRLRRPVVMGEAPAWLRDTLDRFFHTWRVEDATVDISDLT
                     PFEQAALRAAAQIPPGEVRSYAWVATQIGRPKAARAVGRVMARNPLPLLFPCHRVVDS
                     SGALHNYYYGLDMKARILKLEGYRG"
     gene            4281..5807
                     /locus_tag="VFU90_04170"
     CDS             4281..5807
                     /locus_tag="VFU90_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479001.1"
                     /translation="MLFLWFFHACRRLSPRFLMVADHINYLTFEDPGAVNTLRRALKL
                     AHAGDLHGAAETAGVDVAHATVVSEALRRGMRFSIGAEADNDPRARPDAQNIVDAMRP
                     DGLIRSVSFVNVDHPEKGADWAWAFDNEEFAAIHQLVGPDRLWELYVDKLIDDLEKLP
                     ANIVGHFYGPANLGGWPAAKRLEEYEDRLLNVCHARALAVEINTRFLYRENPPDLKKR
                     YLEANSRLARKAKARGVGIAIGSDAHSPKDQGNAFDVILRLLDDAKINELVFPVGGRL
                     ARVALRATREHLEAEAKRRVGHLPGSSITGFSRAELGLPEQAEVPSAREGARAAREAA
                     SRKRPVGSTRAGKRSEAPPPKSAAKSGSRRTAESSTSSIKAKAARTAKPPAAKKAPAK
                     KVPAKKAPPKKAAAKPKAAPKKAAAKPHPRAPIAKPAPAAKRKAPATARPAKKKASPK
                     RVTAVRKAAAKKPVHKPAAKSAKGARTVKAPAKKTVKKTAKTPSKGSATKKRPTAKRR
                     "
     gene            5843..7879
                     /locus_tag="VFU90_04175"
     CDS             5843..7879
                     /locus_tag="VFU90_04175"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5479002.1"
                     /translation="MADIARLRNIAFVGPHHAGKTTLVEAILAYTGAIGRRGSIADGS
                     TVTDHEPEDVAHAQSTTVGFAHAGDDGIDITIVDTPGFVDFFQETKAVLAGVDAAIVV
                     VEADPGRVVQSQGIVDLLESMRMPHLFVVNKMDRPGADFAGTLTALQNAYGRHVVAEQ
                     LPIGSAEQFRGYVDLAEMHARTFDGDRETDAALPDDVREPAQRARGELLEAMADFDDH
                     LMEELLEGVEPPLEEVERDLCSECSHDQIVPVLVASGASGAGVPALVRAMEKWFPSPA
                     DAPKVDAEGRPIAPDPAAPVVVQIVKTAINPQSGKLSIARILSGTLKSDATLTNVTRD
                     GERARLGGLYRLQGKKQEAIGEAGPGMLVALARLESVSTGDTLTSNGHKVLLPRIPFA
                     EPVFAAAIKPKERIDEAKISQMLARIVDEDPSLRLQRADITHELLLLGSGEQHVSIAV
                     ERLSRKYKVEVETAPPSIPYLETITGGTEIHSRYKHQTGGHGQFGDVWLRFEPRQRGN
                     GVTFEDKIVGGVVPRQFIPAVEKGVREALSHGPGGNPVTDLHVTLFDGQYHDVDSSEQ
                     SFKTAAGMGVRDALPKCNPVVLEPIVHVQVEVPTAYTSAVIQQLTGKRGQILGMNPAE
                     RAGFDLVEAYVPQVELSRYITELRTATQGLGTYSWRHERYDPVPGNRVGPKAAV"
     gene            7888..9516
                     /locus_tag="VFU90_04180"
     CDS             7888..9516
                     /locus_tag="VFU90_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5479003.1"
                     /translation="MKRLAALAAAVACAAGCSKVSTQTAGARHSWTQAGVLRVALQQD
                     VKNLNPLLNSNTTDGFIAFLMFEPLLAADAKGNPVPILATAVPTVENGGISRDGLTIA
                     YHLRTDAKWTDGVPVTSKDVQWSWQAIMNPNNNVVSRHGYDYIKSIDTPNPSTVVVHL
                     KTKFSPFVNSFFAMSDQPFPIAPEHVLAKYPNINQIPFNNAPSVSDGPFKFVEWIHGD
                     HVTLARNDGFFMGKPGLDRVELRVIPDENTSVNLLKTHAIDYMFQASPNTYPALQSAT
                     DVKLDYVNINGYEDVQLNLSRPNLTDPRVRLAIAYAIDKSQLVSTLTHGQMTMATEDV
                     PDWMWAFNPTVHSYPHDPVKARDLLRRAGWAPGPGGVMYKNGRPLSLLLVSNNSNATR
                     REASLQLQAMLKQAGIDTEIKYYPGDTLFAPAGMGGILQLGRFDLSLAGWYAGIDPDD
                     SSQFMCQNVPPGGYNYSRYCNPDMQAAQIAALTHYDRPSRMAAYYKIQELLARDNPEI
                     FFWWTRQIEPISVDFKGFDPNPVVESWNAWQWSI"
BASE COUNT         1756 a         3242 c         2982 g         1563 t
ORIGIN      
        1 caagccgcgt tcgtaaacgc cggcgagttc tccgtcatcc attttggcga cgagcttctc
       61 aaccgtgccc accagcacgt tggccggtac attccccaac agggacacgc ctctaccgtt
      121 atccccgtcg gcaggcagct cctcgaagcg gacgaaaatg accctcgtga ttcgcgtcaa
      181 ggtgccgctg gatctccact ccgtcgcggc cgacggctcg ggcgcctttg cgctcgccac
      241 gccgctgcgg gtgcgcgagc tgctggcgct cgcggtacgc gaagccatcg gctcgcgtgc
      301 cccggaagac aagttcgcgc gcagcgttca ccgtacgctc gccggacttg catcgggaga
      361 tttcacgctg gtcatcgacg gccgtacctt cgcggatccc gacgcggtcg tcgtttgcga
      421 cggcaccgcc gacgttcgtt tcttcatcgc ttcgcggcgg cgcgccggag tgcggcgtta
      481 aaccaccaca cgcctccttc ctcagcctcg tagggctgtc gtttcgtctc atcgctcgcc
      541 gctggaagct cttcgttccg gcggtggcgg tcgcgatggg cgtgcaagcc ctcgtcgccg
      601 tcgtgtggcg catcccgcac ggagccgacc tggacggcgc aatcgtcctg ccgattctca
      661 ccacgctcgt ctacggattc gtggccgccg acgcccggcc ggagccggcc gcagcctcga
      721 gcgtttggga acgcgtactc gagcgttcgt gggcggtcat cgtcatcgac ttcgtcgtct
      781 cgtacgttac ggcgctggga ttggcgggaa gcacgtcgcc taacgcgctg gacctgatcg
      841 cggggttgtc ggccttcgtg ctgagcgtgc tgttcgtctt cgccgacgcc agcgccacgg
      901 tggacggcga cgtcggcgtc ttgaccgtga tcccgtacgg attcgttcgc agcgtccaag
      961 cggcatggcg gcgtccggtg cttccccgag cgctggcgat cttttcaatc cagctgctcg
     1021 tcgccgcggt gcagatcgcg ctcggcgcgc tgctgcagcg cggtcacgtc gcggacgcgc
     1081 tgttttggtc ggagattccg ctgctgacgg tggtggtgcc gccgctttcc gccctgacgg
     1141 tgctcgtcta tctcgacgcg cgggccgcgc ccgcgcaatc cgccgagtaa aagcaggcag
     1201 gctgcaacgc ccttgcggcg gatggtagtg tagagaaaag ccatcttctc gaaacggaac
     1261 gcaccatttc ttcccgccgg atcaccgcca cgctcgcctt cgtcgccgcg ttgtcggcaa
     1321 caaccctcgg tgcggctgcc gaccagaaat acgacgtcca cggagaagac gtctatcgcg
     1381 tcggttccag cccttcggcc tcgcgagtcg cctataccgg cgaacagcgt ctactggtcg
     1441 agcgcgacgg cgcgagcacg cgcttcgacg cgcaggccag ttacacgcgc gacgccgacg
     1501 gcgtcaaacg gcggatcgac gcgcggttcg tccaggagct tcttccggac ggcgccttcg
     1561 aggaccggat cgacgacgat cccgactttt tgacgatcct caaccaaccg ttcgcgatcc
     1621 ggctcgacgg cgccacgctt cgcgatctgc agaacttgcg cgcgccggtt ccgttcgacg
     1681 ccagttcgcc gctcggcggc cgggacgtgc tgcgcggcta tctgcgtccg gcgccccccg
     1741 gaaagatctg cggacggccg gtcgtggcgg tcgctttcga agccgaaggg ccgatgacgg
     1801 cgccgatgcc cactcgcacg tcggccacga tgtcgggccg gatgcggatg caagggacgg
     1861 cgtactacgc ccgcagcgac gcgatgctgc tcgcgctcga cgcaacgctc acgatcgtcg
     1921 cgcagctgca cgagggaacg agcgtaacgc cggtccgcgt cgtctaccgg cgcttcattc
     1981 gcgcggcctt gccgagcccg gcgccggcgg ctaaaacgcc cccaccaact ccacttccaa
     2041 cgggtggcgg aacggcacct cccccagctc ggtgagcggc gtaaacgcca aatggttttg
     2101 gtggcaatcc accagcatct cgatgccgcg ctttacgcag cccgtttcct cgacgtattt
     2161 ctcgagcttt ttcttaaagt aggcgcgcgg atcccgcaac accttggcgt agtacggatc
     2221 gtcggtgaga ccttcgttgg gaatgccgtc cttcggtacg agccatacga accacggatc
     2281 ctcgttctgc cggtagccga tcagcgctag cggaatcgcg cgcgccgaat ccagattcgg
     2341 aagaaagctg cacatcgtgt aagcatcgaa cgagatctcc gtgggttcga tggtttgccg
     2401 catagatcgc acctcttcgt aacagcctcg gcggcggcgg cgggacgaaa ttgaaaaacc
     2461 gcttcattca cttgccgcaa gaatgaagcg gttttccgga cgttcgtcgc gagccggcct
     2521 gccggaacaa aagcgccatg gcgacgactt tgcgagccga ggggatgttc ctacgccccg
     2581 gcagcgcgcc ggcgcgggct cggtgggccg gttttacgcc tcaggaattt tccgggcgca
     2641 tgtgaaacca ggcggcaggc ggcagtggtc cgagtcactg tatgagtgtg ctcgccgcac
     2701 cgctccttca gggagtattc gtgccttcag cggttccgac cgggaaaatc gcggccgctc
     2761 ggccggcggc gactgccgtc cccgggatcg aaacgttcga gcgggtcgtc gacgactacc
     2821 agcgccgcct ctacggcttt gccttgcgca tgacggggaa tcgcgaggat gccgaagaga
     2881 tcgtgcagga cgcattcgtc cgcgcttacc gggcgctcgg aaagatgtcg ccggagcagc
     2941 gtgcggagct gcgcctccag ccgtggcttt acacgattac gctcaacgtc acgcggaacc
     3001 ggctgcgaag caagcgcccg tccaacgtag cgctcgacgc gctggcggat cccgacgctc
     3061 tgctgaggga gtcgaacgag ggcccgccgc agccggagac catcgtcgaa cgcaacaccg
     3121 acgtagccct ggtggagcgc gcgctcatgc agctgcccat gcacctccgc gcggccgcga
     3181 ccctacggtt catcgaggga cgcagccatc cggagatagc cgagatactt caccagccga
     3241 tcggcacggt gaaatctcac gttcaccggg ccgtccggat cctgcgacgg atcctcggtc
     3301 cgcaggtcgg acgatttacc cccgaaggag caccaacgaa tgcgctgtcg tgacgtcgag
     3361 attatgtggg acgagatccg cggcgactgt aaggagtcgc tcaaggaaac cgtcgagacg
     3421 cacctgctgg cgtgtccgcc ctgccaggag ctctacgaag agttcgaagg cgtcgcctac
     3481 tgcctctcgt gccttccgca gcccgaaccg tcgtgcaatc ttaccaagaa ggtcgtcgag
     3541 cacatcgcct cgctcaagct caaggaccgt acgccgatcc ttctgtgttc cgtcgaaacc
     3601 ccgatcgggc gcgcctacgt cggcttcaag gagcggcgca ttgcctacgt cggcatcgat
     3661 accggcgatc cgctcgagga agtccgcggc cgcgtcgagc ggcgcttgcg ccgcccggtt
     3721 gtgatgggtg aagctccggc ctggctgcgc gacaccctcg accgcttctt ccacacctgg
     3781 agggtcgaag acgcgacggt ggacatcagc gacctcacgc ctttcgagca ggccgccctg
     3841 cgtgcggccg cgcagatacc gcccggcgaa gtccgctcct acgcttgggt ggcgacgcag
     3901 atcgggcgcc cgaaggcagc gcgcgcggtc gggcgcgtga tggcgcgcaa tccgctgccg
     3961 ctgctatttc cgtgtcatcg cgtcgtcgac tcgtccggcg ccctgcacaa ctactactac
     4021 ggtctcgaca tgaaggcgcg cattctcaag ctggaaggat atcgcggcta gcgatcggcc
     4081 ccgcgtctgc cggaggggaa cgcaaaatcg gcgccaacct tagcggaacg taacgggcga
     4141 acccgcctcg ttccacgcaa ggtaaaggaa gtacatcgct gagcgaccca cgtatcgcgg
     4201 ccatccccga cgttcacgga catgccaagg gggagcaccg gccgtttgcc gaggagcgtc
     4261 cgatcgccta ttcggctcgg atgctctttc tttggttctt ccacgcctgc cggcgcctct
     4321 cgccgcgttt cttgatggtc gccgaccaca tcaactatct gacgttcgag gacccgggcg
     4381 cggtcaatac cctgcgccgc gcactgaagc tggcgcatgc cggcgatctc cacggcgcgg
     4441 ccgaaaccgc cggggtcgac gtcgcccacg cgacggtcgt ctccgaagcg ctgcggaggg
     4501 gaatgcggtt ctcgatcggc gccgaagccg ataacgatcc gcgcgcccgc cccgacgcgc
     4561 agaacatcgt ggatgccatg cgcccggacg gactgatccg ctcggtgagt ttcgtgaacg
     4621 tcgaccaccc cgaaaaaggc gccgactggg cttgggcgtt cgacaacgag gagttcgccg
     4681 cgatccacca gctcgtcggc cccgatcggc tgtgggagct ctacgtcgac aagctgatcg
     4741 acgatctcga gaaactgccc gccaatatcg tcggtcactt ctacggtccg gccaatctcg
     4801 gcggctggcc cgcagcgaag cggctcgaag agtacgaaga ccgcctgctc aacgtctgtc
     4861 acgcgcgcgc tctggccgtc gagataaaca cccggttcct ctatcgagaa aatccgccgg
     4921 atctcaagaa gcgctacctc gaagccaact cgcgcttggc tcgcaaagcc aaggcccgcg
     4981 gcgtcgggat cgcgatcggc tccgacgccc acagccccaa ggaccaagga aacgccttcg
     5041 acgtcatctt gaggcttctc gacgacgcca agatcaacga gctcgtcttt ccggtcggcg
     5101 gacggctggc gcgcgtcgcg ttgcgggcta ctcgcgagca cttggaagcc gaagcgaagc
     5161 ggcgcgtcgg gcatctgccc ggcagcagca tcaccggatt cagccgcgcc gagctcggcc
     5221 tccccgagca agccgaagtc ccatcggctc gcgagggcgc acgggccgcg cgcgaggccg
     5281 cttcacggaa acgtccggtg ggctcgacgc gcgccggtaa gcgctccgaa gcgcccccgc
     5341 cgaagagcgc cgccaagagc ggttcgcgcc ggaccgcgga gtcgtccacg tcgtcgatca
     5401 aagccaaggc cgcacgcacc gcaaagccgc ccgccgccaa gaaagcgccg gccaaaaagg
     5461 ttcccgccaa aaaggcgccg cccaagaagg cggcggccaa accgaaagcc gcgcccaaaa
     5521 aggccgctgc taaaccgcat ccgcgcgccc cgatcgccaa gccggcgccc gcggcgaagc
     5581 gaaaggcgcc ggcaaccgcc cggccggcaa agaaaaaagc atcgccgaag cgggtgacgg
     5641 ccgtccggaa agcggccgcc aagaagccgg ttcacaaacc cgccgcgaaa tcggcaaagg
     5701 gcgcgcgcac cgtcaaagct ccggccaaaa aaaccgtgaa gaagaccgcg aagacgccga
     5761 gcaagggttc ggcgacaaag aaaagaccaa cggccaagcg tcgttaagac gccagccacc
     5821 gtaagaccga gaggagagtg ggatggcgga tatcgctcgc ctgcgcaata tcgctttcgt
     5881 cggaccgcac catgcgggaa aaacgaccct ggtcgaggcg attctcgcct ataccggtgc
     5941 catcgggcgg cgcggttcga tcgccgacgg atccaccgtc accgaccacg agcccgagga
     6001 cgtcgcgcac gcccaatcga cgacggtcgg attcgcgcac gccggcgacg acggcatcga
     6061 catcacgatc gtcgacaccc ccgggttcgt cgatttcttc caagagacca aagcggtgct
     6121 cgccggcgtc gacgccgcga tcgtcgtcgt cgaagcggat cccggccggg tggtgcagtc
     6181 gcaagggatc gtcgacctcc tcgagtcgat gcggatgccg catctgttcg tcgtcaacaa
     6241 gatggaccgt ccgggagccg actttgcggg cacgctgacg gctttacaaa acgcctacgg
     6301 ccggcacgtc gtcgccgaac agctgccgat cggcagcgcc gagcagtttc gcggctacgt
     6361 ggatctcgcg gagatgcacg cgcgtacttt cgacggcgat cgcgagaccg acgccgcgct
     6421 gcccgacgac gtccgcgagc cggcgcagcg cgctcgcggc gagctgctcg aggcgatggc
     6481 cgacttcgac gatcacttga tggaggagct gctcgagggc gtggaaccgc cgctcgagga
     6541 agtggagcgc gacctctgct ccgagtgttc gcacgatcaa atcgttccgg tgctggtggc
     6601 ctccggcgcc tcgggggccg gcgttccggc gttggtgcgc gcgatggaaa aatggttccc
     6661 gtcgcccgcg gacgccccca aagtcgacgc cgaaggccgt ccgatcgcgc ccgatcccgc
     6721 ggctccggtc gtcgtccaga tcgtgaagac ggcgatcaat ccgcaaagcg gaaaactttc
     6781 gatcgcccgc atcctgtcgg ggactctcaa gtcggatgcg acgctcacga acgtcacccg
     6841 cgacggcgag cgcgcgcgcc tcggcggcct ctaccggctg cagggcaaga aacaagaagc
     6901 gatcggcgag gccggtcccg ggatgctcgt cgcgctcgcg cggctggaaa gcgtttcgac
     6961 cggggatacg ctgacgtcga acggtcacaa agtgctgctg ccgcgcatcc ccttcgccga
     7021 gccggtcttc gccgcagcga tcaaaccgaa ggaacgcatc gacgaggcga agatctcgca
     7081 gatgctggcg cgcatcgtcg acgaggatcc gtcgctgcgg ttgcagcgtg ccgacatcac
     7141 gcacgaactc ttgctgctgg gaagcggcga gcagcacgtg tcgatcgccg tcgagcggct
     7201 ctcgcgcaaa tacaaagtcg aagtcgaaac cgctccgccc tcgatcccgt atctggagac
     7261 gatcaccggt ggtaccgaaa tccactcgcg ctacaagcac caaaccggcg gacacggcca
     7321 gttcggcgac gtgtggctgc gcttcgagcc gcgccaacgc ggtaacggcg tgaccttcga
     7381 agacaagatc gtcggcggcg tggtcccgcg gcaattcatc cccgcggtgg aaaagggcgt
     7441 gcgggaagcg ctctcgcacg gtcccggcgg aaatccggtt accgacctgc acgtcacgct
     7501 gttcgacggg cagtaccacg acgtcgactc cagcgagcag tcctttaaga ccgccgccgg
     7561 gatgggcgtt cgcgacgcgc tcccgaagtg caatccggtc gtactcgaac cgatcgtcca
     7621 cgtgcaagtc gaggtgccga cggcgtacac gtcggcggtg attcagcagc tcaccggcaa
     7681 acgcgggcag atcctcggca tgaacccggc cgagcgcgcc ggcttcgacc tggtggaggc
     7741 ctacgttccg caagtcgagc tgtcgcgcta catcaccgaa cttcgcacgg cgactcaggg
     7801 cttgggcacc tattcctggc gtcacgaacg ctacgatccg gtgccgggaa accgcgtcgg
     7861 acccaaagca gcggtttagg gcaagaaatg aaacgactcg ccgcgctcgc ggccgccgtc
     7921 gcgtgcgcgg ccggctgcag caaggtctcg acgcaaaccg ccggggcccg ccattcgtgg
     7981 acccaggccg gcgtgttgcg cgtcgcgctt caacaagacg tcaagaacct caatccgctg
     8041 ctcaactcga acacgaccga cggctttatc gcgtttttga tgttcgagcc gctgctcgcc
     8101 gccgacgcca aaggcaatcc ggtcccgatc ttggcgacgg cggttccgac ggtggaaaac
     8161 ggcggcatct cgcgcgacgg cttgacgatc gcctaccatt tgcggaccga tgcgaagtgg
     8221 accgacggcg ttccggtgac gtcgaaggac gtccagtggt cgtggcaagc gatcatgaac
     8281 cccaacaaca acgtcgtttc gcgccacgga tacgactaca tcaagtcgat cgacactccc
     8341 aacccttcga ccgtcgtggt tcacctgaaa accaaattct cgccgttcgt aaacagcttc
     8401 ttcgcgatga gcgaccagcc gtttccaatc gcgccggagc acgtgctggc aaagtatccc
     8461 aacatcaacc agattccgtt caacaacgcg ccgtccgtca gcgacgggcc gttcaagttc
     8521 gtggagtgga tccacggcga tcacgtgacc ctcgcgcgca acgacggctt tttcatgggg
     8581 aagcccgggc tggaccgagt cgagcttcgc gtaattcccg acgagaacac gtcggtgaac
     8641 ttgttgaaaa cccacgcgat cgactacatg tttcaggcgt cgcccaacac gtatccggcg
     8701 ctgcaaagcg ccaccgacgt caagctggac tacgtgaata tcaacggcta cgaagacgtc
     8761 cagctcaacc tgtcgcggcc gaacctcacc gatccgcgcg tccggctggc aatcgcctac
     8821 gccatcgaca agtcgcagct cgtatcgacg ctcacccacg ggcagatgac gatggccacc
     8881 gaggacgtcc cggactggat gtgggctttc aatccgaccg tgcactcgta cccgcacgac
     8941 ccggtcaaag cccgtgacct gctccggcgc gcgggctggg cacctggacc cggcggcgtc
     9001 atgtacaaga acgggcgccc gctctcgctt ttgctcgtct cgaataactc caacgccacc
     9061 cgccgcgaag cgagcctgca gctccaagcg atgctcaaac aagccggaat cgacaccgag
     9121 atcaagtact atccgggcga cacgctgttc gcgcccgccg gaatgggcgg catcttgcag
     9181 ctcggccgat tcgatctgtc gctggccggc tggtatgccg gcatcgatcc cgacgacagc
     9241 tcgcagttca tgtgccaaaa cgttccgccc ggcggctaca actactcgcg ctactgcaac
     9301 cccgacatgc aggcagcgca aatcgccgcg ctcacccact acgaccgccc ctcgcggatg
     9361 gcggcatact ataaaattca agagctgctg gcgcgcgaca atcccgagat ctttttttgg
     9421 tggacgcggc aaatcgagcc gatcagcgtc gacttcaaag gattcgatcc caatccggtg
     9481 gtcgagtcgt ggaacgcgtg gcagtggagt atctagcgga gcgtccggcc tgcggaacct
     9541 cga
//
LOCUS       DASUCJ010000347         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_446951, whole genome shotgun sequence.
ACCESSION   DASUCJ010000347 DASUCJ010000000
VERSION     DASUCJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446951"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /gene="alaS"
                     /locus_tag="VFU90_04185"
     CDS             complement(<1..1394)
                     /gene="alaS"
                     /locus_tag="VFU90_04185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5479004.1"
                     /translation="MKSQELRQAWIDFFVAKQHRLLPPATLVPHELSTTLFTIAGMEQ
                     FVPVFLGEQPAPAPRAVTIQRCLRVAGAKSDIENVGRTGRHGTFLEMLGNFSFGDYYK
                     SEAIAWAWEFATQVLRLDPARLYVTVHTGDDEAERIWTEEIGLDASRVSRFDDDNFWT
                     MGPTGPCGPCTELFYDLGERYADGPGDDGPNKGSRFVEFWNVVFQQYNRTSDGKLSEL
                     PRKAIDTGAGFERMLAICNGVASMYDTDLFADLVAAQPPAGRTALSQAEQRVRRNVVA
                     DHARAVTFLLNDGVYPSNTDRGYVLRFLIRRAIRQGRLLGYPKGFFTALVPAVVTSLE
                     PGYPELRQNASRIAAALDAEERAFERTLERGMAMLDDRIARAIEDGARTIDGAGAFLL
                     HDTYGFPVELTREIAAESGVTVDVVGFDALMNEQRERARDDAAAKRAAVGVTDLPAVR
                     SEFTGYGGLEGEGTV"
     gene            complement(1451..>1914)
                     /locus_tag="VFU90_04190"
     CDS             complement(1451..>1914)
                     /locus_tag="VFU90_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5479005.1"
                     /translation="AVKVPRRLQELNLAVNRAGDKLAIELGRSPTVGELAERLGAAEE
                     DILEAQELGQAYNLLSLDSEVAGETDKKSQTLSDTVGVTDAGLELLEDRANLERAFGV
                     LTGRERVIIYLRFYESVSQTEIAKRLNVSQMHVSRLQAKALEKLRAVLGSA"
BASE COUNT          325 a          648 c          608 g          333 t
ORIGIN      
        1 accgtgccct cgccctcgag accgccgtag ccggtaaact cgctgcgaac ggccggcaga
       61 tcggtgacgc cgaccgccgc gcgtttggcg gcggcgtcgt cgcgggcgcg ctcgcgctgc
      121 tcgttcatca gcgcgtcgaa accgacgacg tcgaccgtca cgccgctttc cgccgcaatc
      181 tctcgcgtca actcgaccgg aaagccgtag gtgtcgtgca acagaaaggc gcccgcgccg
      241 tcgatcgtcc gggcaccgtc ctcgatcgcc cgtgcgatcc ggtcgtcgag catcgccatt
      301 ccgcgttcca acgtccgttc gaacgcccgc tcttcggcgt cgagcgccgc cgcgattcgc
      361 gaagcgtttt ggcgcagctc cggatagccc ggctccagcg acgtaacgac ggccggaacc
      421 agcgccgtga aaaacccttt gggatagccg agcagccggc cttggcggat cgcgcgccgg
      481 atcagaaagc gcaggacgta tccgcgatcg gtattcgacg gatacacgcc gtcgttgagg
      541 aggaacgtca ccgcgcgcgc gtgatcggcg acgacgttgc ggcgcacgcg ctgctcggct
      601 tgcgagagcg cggtccgccc ggccggcggc tgcgccgcga ccaaatcggc gaacaggtcg
      661 gtatcgtaca tcgatgcgac gccgttgcaa atcgcgagca ttcgctcgaa acccgcgccg
      721 gtgtcgatcg ctttgcgcgg aagttcgctg agttttccgt cggacgttcg attgtattgc
      781 tggaacacga cgttccaaaa ctccacgaag cggctgcctt tattcggacc gtcgtcgccg
      841 ggaccgtccg cgtagcgctc gccgagatcg tagaacagct cggtgcaggg accgcacggc
      901 ccggtcggcc ccatcgtcca aaagttgtcg tcgtcgaaac gcgagacgcg cgatgcatcc
      961 agaccgatct cttcggtcca aatgcgctcg gcctcgtcgt cgccggtgtg gacggtcacg
     1021 tacaaacgcg ccgggtcgag ccgcagcacc tgcgtcgcga attcccacgc ccaggcgatc
     1081 gcctcggatt tgtaatagtc gccgaagctg aagttgccga gcatttccaa aaacgttccg
     1141 tgacggccgg tccgcccgac gttttcgatg tcgctcttgg cgcccgcgac gcgcaggcag
     1201 cgctggatcg tcaccgcgcg cggcgccggt gccggctgct cccctaagaa caccgggacg
     1261 aactgctcca tcccggcaat cgtaaagagc gtcgtcgaga gttcgtgcgg tacgagggtt
     1321 gccggcggca acagccggtg ctgcttggcg acgaagaagt cgatccaggc ttggcggagt
     1381 tcctgactct tcatgggcgt tgcagaatga tagcagaaat ccccgggggg aggctatcgc
     1441 caaatccggc ttaggcgctc ccgagaaccg cccggagctt ctccaacgcc ttggcctgca
     1501 ggcgcgaaac gtgcatttgc gacacgttca gacgcttcgc gatctcggtc tgcgagacgg
     1561 actcgtaaaa gcgcaggtag atgatcaccc gctcgcggcc cgtaagcacc ccgaacgcgc
     1621 gctccaagtt ggcgcgatct tccaacagct ccaggccggc gtcggtgacg ccgaccgtat
     1681 ccgacagcgt ctgcgacttc ttatcggttt cgccggcaac ttcgctgtcg agcgagagca
     1741 ggttgtaggc ctgcccgagc tcctgagctt ccaggatgtc ctcttcggcg gcgcccagcc
     1801 gttcggccag ctcgccgacg gtcgggctgc ggcccagctc gatggccagc ttgtcgccgg
     1861 cacgattgac cgcgaggttc agctcctgga gacggcgggg aaccttcacc gccc
//
LOCUS       DASUCJ010000348         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1134700, whole genome shotgun sequence.
ACCESSION   DASUCJ010000348 DASUCJ010000000
VERSION     DASUCJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134700"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFU90_04195"
     CDS             <1..955
                     /locus_tag="VFU90_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479006.1"
                     /translation="SQAEASASPSPPPQTPYSYPNSGGGSMNDAPAAFPLDPAAPGLC
                     GASVADDYGQVAQTSVQVMGWLTAAYAGKSYVRGTKPALALPASAFPQRGATATIGIS
                     KTYDAEPLQPAVAFDTACSPYVAFAARPGTTPRQPSRQPATATVALTLITMPKSKIDC
                     GGTIYDQYRGSLDGEGIPFNATIGQSPCPNSGNAQTGPRDGVCYDLYFIGTGMTQTGG
                     WTEESEVGLYVAHGTDGDALYRWVVDNGSCSIQNLIGTDFATWSVLLGNGDTTPPPLA
                     TPQPIPNPAGFGLPAIPDAVDVTAAPDPKPTVPPSFLCKGS"
     gene            complement(965..>1431)
                     /locus_tag="VFU90_04200"
     CDS             complement(965..>1431)
                     /locus_tag="VFU90_04200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479007.1"
                     /translation="TSTGMWERILATNLTGTFLCTREAVEDMIVAKAGRIVNVASIAG
                     LAGAPYIAAYCASKHGVVGFTRAIAAEFEGTGITANAVCPGYTETDMMRKALGNIVKH
                     TGAEEDAARAQLARSNPEGRIATVEEVADAVVDLILGDRTGAAVVVPGGVVA"
BASE COUNT          238 a          510 c          441 g          242 t
ORIGIN      
        1 ctcgcaggcc gaagcctccg cttcgccctc gccgcctcca caaacgccct actcctatcc
       61 gaattcgggc ggcggcagca tgaacgacgc tccggcagct tttccgctcg acccggcggc
      121 gcccggactg tgcggcgctt ccgtcgccga cgactacggg caggtcgcgc agacgtcggt
      181 tcaagtgatg ggatggctta ccgcagcgta cgctggaaag agttacgttc gcggaacgaa
      241 gccggcgctc gcgctgccgg cctcggcctt tccgcaacgc ggcgcgaccg cgaccatcgg
      301 catctccaag acgtacgatg ccgaaccgct gcagccggcg gtcgcgttcg acacggcctg
      361 ttcgccgtac gtcgcctttg ccgcgcggcc cggtacgacg ccgcgtcagc cgtcgcggca
      421 gcctgcgacc gccaccgtag cgctgacgct gatcaccatg ccgaaatcga aaattgactg
      481 cggcgggacg atctacgatc agtaccgcgg ctcgctcgat ggcgaaggca tcccgttcaa
      541 tgcgacgatt ggccagtcgc cgtgtcccaa cagcggaaac gcgcaaacgg ggccgcgcga
      601 cggcgtgtgc tacgatttgt acttcatcgg gacgggcatg acgcaaaccg gaggatggac
      661 cgaagaaagc gaggtcggcc tctacgtcgc gcatgggacc gacggcgacg cgctttaccg
      721 ctgggtcgtc gacaacggct cgtgctcgat tcagaatctc atcggcacgg acttcgcgac
      781 ctggagcgtg ctgctcggca acggcgatac gacgccgccg ccgctcgcga cgccgcaacc
      841 catccccaac cccgccggct tcggactgcc ggctatcccc gacgccgtcg acgttaccgc
      901 cgcccccgat ccgaagccga cggttccgcc gtcgttcttg tgcaaaggct cctaaagagt
      961 tcggctaggc gaccaccccg ccggggacga cgacggcggc accggtccgg tcacccaata
     1021 tcaagtcgac gaccgcatcg gccacctctt cgacggtcgc gattcttccc tccggattgg
     1081 aacgtgcgag ctgggcgcgc gcggcgtctt cttcggcgcc ggtgtgcttg acgatattgc
     1141 cgagcgcctt acgcatcata tccgtctcgg tgtagccggg gcagacggcg ttggccgtta
     1201 tccccgtgcc ctcgaactcg gccgcgatcg cgcgggtgaa tccgacgaca ccgtgtttgc
     1261 tcgcgcagta ggccgcgata tacggcgcgc ctgccagacc ggcgatgctt gcaacgttga
     1321 cgatccgccc cgcctttgcg acgatcatat cttccaccgc ctcgcgcgtg cacaggaacg
     1381 ttccggtaag gttggtcgca aggatccgct cccacattcc cgtcgaggtc c
//
LOCUS       DASUCJ010000349         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1440471, whole genome shotgun sequence.
ACCESSION   DASUCJ010000349 DASUCJ010000000
VERSION     DASUCJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440471"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2203
                     /locus_tag="VFU90_04205"
     CDS             <1..>2203
                     /locus_tag="VFU90_04205"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEU5479008.1"
                     /translation="KPGVIAINRRGQSSLWAYGISGDLPIVLLQLADLANIELARQLV
                     QAHRYWRLFGLAADLVILGDDRDGQSSALQERITKLVAACGGTEHVDRPGGIFVRPAG
                     KVSEDDRVLLQSAARVVINDADGSLAQQLERRGAEKNPTPTLPATRTGDPGIPAPAES
                     PPPMVDRDPIFGNGLGGFTSDGREYVITIASGRMTPAPWINVLANRTFGTLISESGRA
                     NTWSQNAQQFRLTPWSNDPVGDANTEAFYLRDEESGRFWSPTLLPSGGTTPYVTRHGF
                     GYSVFEHREEGIDSELAVYVAIDAPVKFVMLKLNNRSGRRRQLSVTGYLEWVLGDERS
                     KTQMHVGTERDADSAALFAHNAYNTEFAGRIAFFDVDEMTAASVCGDRREFLGRNGTL
                     RSPAAMSALALSGIVGAALDPCAAIRLPFDLAVGQTRELVFRLGAGASVDEARRLVRR
                     WRGADAAREALTVVKRYWEQTLGAVQVETPERSLDLLANGWLVYQILACRLWARNAFY
                     QSSGAFGFRDQLQDVMALVHAAPELVREHLLLCASRQFPEGDVQHWWHPQSGRGVRTR
                     FSDDLVWLPYVIDHYVNIACDTGIFDVEVPYITMRELNPDEHEVYDLPKVSDQTGTIY
                     DHCVRALRRATTKGQHGLPLIGSGDWNDGMNRVGIEGKGESVWLAWFLITTLRRFAEH
                     AERRGDAAVRDEMLAKADGYAEAIERSAWDGEWYRRAYFDDGSPLGSRTSDE"
BASE COUNT          393 a          677 c          749 g          384 t
ORIGIN      
        1 caagcccggc gtgatcgcca tcaaccgtag gggtcagtcg agcctgtggg cttacgggat
       61 ttcgggcgac ctgccgatcg tgctgctgca actcgcggac ctcgcgaaca tcgagctcgc
      121 gcgccaactc gtgcaggcgc acaggtactg gcggctcttt ggactggcag cggacctcgt
      181 gatcctcggc gacgatcgag acggtcagtc gtcggcactt caggaacgaa tcacgaagct
      241 ggtcgccgca tgcggcggta ccgagcacgt cgaccggccc ggcgggatct tcgttcgccc
      301 agccggcaag gtgtccgaag acgatcgcgt tcttctgcag tcggccgcgc gcgtggtcat
      361 caacgacgcc gacgggtcgc tcgcgcagca gctcgagcgg cgcggcgcag agaaaaaccc
      421 gacgccaacg ctgccggcga cgcgtaccgg cgaccccgga atcccggccc cggccgagtc
      481 gcctcctccg atggtcgacc gcgatccgat cttcggcaac gggctcggcg ggtttacttc
      541 ggacgggcgc gagtacgtca tcacgatcgc gtccgggcgg atgacgcctg cgccgtggat
      601 caacgtgctg gcgaaccgga ccttcggaac cctgatttcg gagagcggaa gagcgaatac
      661 atggagccaa aacgcgcagc agttccgact cacgccgtgg tcgaacgatc ctgtcggcga
      721 tgcgaatacc gaagcgttct atctccgcga cgaggagagc gggcgtttct ggtcgcccac
      781 gctgctgccg agcggtggta cgacgccgta cgtgacgcgc cacggcttcg gctacagcgt
      841 tttcgagcac cgggaggagg gtatcgactc cgagctcgcc gtgtacgtcg cgatcgacgc
      901 gccggtaaag ttcgtgatgt tgaagctgaa caaccggtcg gggcgccggc ggcagctctc
      961 cgtcaccggg tatctggaat gggtgctcgg cgacgagcgc tcgaaaacgc agatgcacgt
     1021 cggcaccgag cgcgacgccg atagcgcggc attgttcgcg cacaatgcct ataacaccga
     1081 gttcgccggg cgcatcgcgt tcttcgacgt cgacgagatg acggccgcga gcgtctgcgg
     1141 cgatcgccgc gagttcctcg gacgcaacgg cacgctgcgc agtcctgctg caatgagcgc
     1201 gctggccctt tccggcatcg tgggtgccgc actcgatccg tgcgccgcga tccggcttcc
     1261 gttcgacctc gctgtcgggc aaacgcgcga gctcgtcttc cggctcggtg ccggagcgag
     1321 cgtcgacgag gcacgtcggt tggttcgccg ttggcgtggc gcggatgccg cgcgcgaagc
     1381 gttgacggtt gtgaagcgat attgggagca aacgctcggc gcagtccagg tcgaaacccc
     1441 ggaacggtcc ctcgacctat tggctaatgg ctggctcgtc taccagattc tggcgtgccg
     1501 cttgtgggcg cgcaacgcgt tctatcagtc gagcggcgcg ttcggcttcc gcgatcaact
     1561 gcaggacgta atggcgctcg tccacgccgc accggagctc gtgcgcgagc acctgctgct
     1621 ctgcgcatcg cgccagtttc cggaaggcga cgttcagcat tggtggcatc cacaaagcgg
     1681 gcgcggtgtg cgcacgcgat tctcggatga cctggtgtgg ctgccatacg tgatcgatca
     1741 ctacgtcaat atcgcgtgcg atacgggaat cttcgatgtc gaggttccgt atatcacgat
     1801 gcgcgagctg aatccggacg agcacgaggt ctacgatctg ccgaaggtct ccgaccagac
     1861 aggcacgatc tacgatcact gtgttagagc tttgcgcaga gcaacgacca agggacagca
     1921 cggcctgccg ctgatcggca gtggcgactg gaacgacggg atgaaccggg tcggtatcga
     1981 agggaaaggc gaaagtgtct ggctcgcctg gttcctgata acgacgctgc gaagattcgc
     2041 tgagcatgcg gagcgtcgcg gcgatgcggc ggtgcgggac gaaatgctgg cgaaggccga
     2101 tgggtacgcg gaagcaatcg agcgctcggc gtgggatggc gaatggtatc gtcgcgcgta
     2161 tttcgacgat ggctcgccac ttggctcaag aaccagcgac gag
//
LOCUS       DASUCJ010000350         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_3914, whole genome shotgun sequence.
ACCESSION   DASUCJ010000350 DASUCJ010000000
VERSION     DASUCJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFU90_04210"
     CDS             complement(<1..352)
                     /locus_tag="VFU90_04210"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5479009.1"
                     /translation="MSRHRPFARAAVLGALLCGASPAIARAQKAPDWAAFDKYVAKAA
                     RDWRVPGMAIAVVKDDSLVFAKGYGVIELGKPEPVNEHTRFAIGSTTKAMTSAALAML
                     VDEGKISWDERVIDI"
     gene            complement(349..>1673)
                     /locus_tag="VFU90_04215"
     CDS             complement(349..>1673)
                     /locus_tag="VFU90_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5479010.1"
                     /translation="HWGIVPTDKPATPGEGSLKVDQLETMVDPKAEWAEIFRETWRTQ
                     REFFYDAKMHGNDWNQIYDKYKVFVPYLEHRADLDYLIASVGGELTVGHSYIESEGDL
                     PDTAHVSVGLLGADLALDHDRYRIQHIYTAGSWNPHLQAPLAVPGLKVAEGDYILEVN
                     GNALAAPTNPYSLFVGTAGKPTTLRVSHSPSTAGSWLVTVEPIPDDEQLRTQAWVDEN
                     RRTVDKLSGGRLAYVWLPNTSVAGFTAFNRYYFAQQDKQGVVVDERYNQGGSVADYIV
                     DQMSRQLMGYFAERAGDVVTMPMVGIMGPKVMIVNESAGSGGDALPYYFRVAKLGPLV
                     GTRTWGGLVGTLGVPLSVDNGAITAPDLAFYDVNGKWAVENEGITPDIEVTETPADVI
                     HGHDPQLERAVAEALKLLKEHPAPNAPRPAPIDRVTTGVATTRSGGNQ"
BASE COUNT          274 a          577 c          519 g          303 t
ORIGIN      
        1 gaatatcgat gacgcgttca tcccagctga tcttgccctc gtccacgagc atcgccaacg
       61 ccgccgacgt catcgccttc gtggtggatc cgatcgcgaa tcgcgtatgc tcgttgacgg
      121 gttccggctt gcctaactcg atcacgccgt agcccttcgc gaacacgagc gaatcgtcct
      181 tcaccaccgc gatggccatc cccggcaccc gccagtcgcg cgcggccttg gccacgtact
      241 tgtcgaacgc ggcccagtcg ggcgccttct gcgcgcgagc gatcgcgggc gatgcgccgc
      301 aaagcagcgc gcccaacacg gcggcgcgcg cgaacggcct atgcctgctc attgattgcc
      361 gcccgagcgc gtggtggcga cgcccgtcgt cacacggtcg atcggcgccg gccgcggagc
      421 gttaggcgcg gggtgctcct tgagcagctt gagcgcttca gccaccgcgc gctcgagctg
      481 cggatcgtgg ccgtgaatca cgtcggccgg cgtctccgtc acctcgatgt ccggcgtgat
      541 gccttcgttc tccaccgccc acttgccgtt gacatcgtag aacgccaggt cgggcgcggt
      601 gatcgcgccg ttgtcgacgc tcaagggtac acccagcgtg cccaccaatc cgccccacgt
      661 gcgcgtgccc acgagcgggc cgagcttcgc gacgcggaag tagtacggca acgcgtcgcc
      721 acccgaaccg gcagactcgt tgacgatcat caccttcgga cccatgatgc cgaccatcgg
      781 catcgtcacc acatcgcccg cccgctcggc gaaatatccc atgagctgcc gcgacatctg
      841 atcgacgatg tagtcggcaa ccgaaccgcc ctgattgtac cgctcgtcga ccaccacgcc
      901 ctgcttgtcc tgctgagcga aatagtagcg gttgaacgcg gtgaatccgg caaccgacgt
      961 gttcggcagc cacacgtagg cgagcctgcc gccggacagc ttgtcgacgg tgcgccggtt
     1021 ctcatccacc cacgcctgcg tgcgcagctg ttcgtcgtcc ggaatcggct cgacggtgac
     1081 gagccacgaa ccggccgtcg acggcgagtg actaacgcga agcgtggtcg gcttgcccgc
     1141 ggtgccgacg aacaagctgt acggattcgt cggagccgcg agcgcgttgc cgttcacctc
     1201 gaggatatag tcgccttcgg ccactttgag gccgggcacg gcgagcggcg cctgcaagtg
     1261 cggattccaa ctgccggcgg tgtagatgtg ttggatgcgg tagcggtcgt ggtcgagcgc
     1321 gagatcggcg ccgagcagtc cgacgctcac gtgcgcggta tcgggcaggt cgccctcgga
     1381 ttcgatgtac gagtgcccaa cggtgagctc gccgcccacc gacgcgatca gatagtcaag
     1441 atccgcgcga tgctccaggt acggcacgaa gaccttgtac ttgtcgtaga tctggttcca
     1501 gtcgttgccg tgcatcttcg cgtcgtagaa gaactctcgc tgcgtcctcc acgtctcgcg
     1561 gaagatctcc gcccactccg ccttcggatc gaccatcgtc tccaactggt cgaccttgag
     1621 cgatccctcg ccgggcgtgg ccggtttgtc ggtgggaacg atgccccaat gat
//
LOCUS       DASUCJ010000351         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_278862, whole genome shotgun sequence.
ACCESSION   DASUCJ010000351 DASUCJ010000000
VERSION     DASUCJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278862"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFU90_04220"
     CDS             complement(<1..322)
                     /locus_tag="VFU90_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5479011.1"
                     /translation="MALAGAAVTLLYAAGAVAGFYTHATAQGTLHEPSLSARVTIARD
                     DRGIPHIVAASERDLFFAQGFAEGSDRLFQMELTRRYALGTVAEVLGPRALSLDEEQR
                     YYDVG"
     gene            complement(447..1169)
                     /gene="aqpZ"
                     /locus_tag="VFU90_04225"
     CDS             complement(447..1169)
                     /gene="aqpZ"
                     /locus_tag="VFU90_04225"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEU5479012.1"
                     /translation="MPIAKRLAAEFLGTFWLVFGGCGSAIFAAVFLTPDKVPVGIGFA
                     GVGLAFGLTVLTGVYALGPISGGHFNPAVTVGLWVGRRFDGKDVIPYVIVQCVAAICA
                     AAVLFLIVSGKTGFAGAGNFAANGYGDHSPGGYNLVSCFVAEVVLTFFFLAVILGSTD
                     RRAPAGFAGLAIGLCLTLIHFVDIPITNASVNPARSLGPALFAQGGWAIPQLWLFWVA
                     PLIGAAIAGIVYSQMFAEKGTA"
     gene            complement(1281..1646)
                     /locus_tag="VFU90_04230"
     CDS             complement(1281..1646)
                     /locus_tag="VFU90_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479013.1"
                     /translation="MAVNVTEYFNKMQQEGLAGLKQTQDATIAAFEKFRAFGSEFSEK
                     PGTVPTFENMPTPTQFVEMSFGFAAQLLELRKAYTLKIAEMLVDTQKQAEANFKQATA
                     TVQNGSVSAPISKPVGSNK"
BASE COUNT          326 a          581 c          534 g          276 t
ORIGIN      
        1 cccccacgtc gtagtagcgc tgctcctcgt cgaggctcag agcgcgcggc cccagcacct
       61 ccgccaccgt tccgagcgca taccggcgcg tgagctccat ctggaacagc cgatccgagc
      121 cttcggcaaa gccttgggca aagaacaggt cgcgctccga agcggcgacg atgtgcggta
      181 tgccgcggtc gtcgcgtgcg atcgttacgc gcgccgacag cgagggttcg tgcaacgtac
      241 cctgcgcggt tgcgtgcgtg taaaacccgg cgacggcgcc cgcggcgtac agcagcgtca
      301 ccgccgcgcc cgcgagcgcc attccgatga ggtgccggta cgcccgcccg gcgaccgcgt
      361 cgaagagcgc gaccggccgg cagaggtctc gaacgagagc ctcgcgctgc cgggccgtat
      421 atcgtcgctc tgccgcgcgc gtaagcctac gccgttcctt tctccgcaaa catctgggaa
      481 tagacgatcc ccgcgatcgc cgccccgatc agcggagcca cccagaacag ccagagctgc
      541 ggaatcgccc agccgccctg cgcgaacagc gccgggccca ggctgcgggc cgggttgacc
      601 gacgcgttgg tgatcggaat gtcgacgaag tgaatcagcg tcaggcacag cccgatcgcg
      661 agccccgcga atcccgccgg ggcgcggcgg tcggtgctgc cgaggattac ggccaggaag
      721 aaaaacgtca agacgacttc tgcaacgaag cacgagacga gattgtaacc gccgggcgag
      781 tgatccccgt atccgttagc cgcgaagttc cccgccccgg cgaagccggt cttcccggaa
      841 acgatcagga aaagcaccgc cgcggcgcag atcgccgcca cgcactgcac gatcacgtac
      901 gggatcacgt ccttgccgtc gaaccgccgc ccaacccaca gcccgacggt gacggccgga
      961 ttgaaatgac cgcccgaaat cgggccgagc gcgtagactc ccgtcaggac cgtcaaaccg
     1021 aacgccagcc ccacgccggc aaaaccgatg ccgaccggca ccttgtcggg cgtcagaaag
     1081 accgccgcaa aaatggcact tccgcagcca ccgaatacca gccaaaacgt tccgaggaac
     1141 tccgccgcaa ggcgctttgc aatgggcatg tcggccctcc ttctgcgcgc attgtagcaa
     1201 cgcaagcgca gaaaaagaaa gcgcccggct gtaaagccgg gtgcttcccg cgtcacctgg
     1261 tagggcgaac cctacgagga ttacttgttc gagccgaccg gcttcgagat gggcgcgctg
     1321 acgcttccgt tctggacggt cgccgtcgcc tgcttgaagt tggcttcggc ctgcttttgc
     1381 gtatcgacga gcatctcggc gatcttcagc gtgtacgcct tgcgcagctc gaggagctgg
     1441 gcggcgaagc cgaacgacat ctcgacgaac tgcgtcggcg tcggcatatt ttcgaaggtc
     1501 gggacggtgc cgggcttctc cgagaactcg ctgccgaagg cgcggaactt ctcgaaggcc
     1561 gcgatcgtcg cgtcctgggt ctgcttcagg ccggcgagac cctcttgctg cattttgttg
     1621 aaatactcgg tgacgttgac ggccatggat tgctctcctt agggtgcttt gctgcttctc
     1681 agtgctaagt ataccaagca cccgcggtat ctgtcaa
//
LOCUS       DASUCJ010000352         1358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1241850, whole genome shotgun sequence.
ACCESSION   DASUCJ010000352 DASUCJ010000000
VERSION     DASUCJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241850"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFU90_04235"
     CDS             complement(<1..314)
                     /locus_tag="VFU90_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479014.1"
                     /translation="MAANEAADAELNKHRWLILLGLITAAIMEVLDTTIVNVALPQMA
                     GNLGATFDEIGWVATAYILSNVIVLPMTAFFTARFGRRNYLTASIAIFAFASFMCGTS
                     HSL"
     gene            complement(503..>1358)
                     /locus_tag="VFU90_04240"
     CDS             complement(503..>1358)
                     /locus_tag="VFU90_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU5479015.1"
                     /translation="AMLWAPCAGPILGLVIAAAAASGAGVRTASLYLAFAAGAATSLA
                     VVLAAGGRVLERLRGAAGAERWARRALGAGTLAVVLLLASGRDAAVFAHAGIATAPAE
                     QRLVALLHGGDGVGGRDVAVRTTPADPPSLPPLGVEGSFPGFTGGGAWINSAPLTPAS
                     LRGKVVMVNFWTFLCYNCLNALPHVEALERKYRDRGLVVVGVHTPEFPTETDEANVRD
                     AVKRLGVAYPVVMDNGYAIWKAFSNQYWPAAFLIDKSGKIRYHHFGEGAYDTEDRVVS
                     QLLSEPAP"
BASE COUNT          237 a          491 c          433 g          197 t
ORIGIN      
        1 aggctgtgcg acgtgccgca catgaacgac gcgaacgcga agatcgcgat cgacgccgtg
       61 agatagttgc gccgcccgaa tcgcgccgtg aagaacgccg tcatcggcag cacgatcacg
      121 ttcgagagaa tgtacgcggt ggcgacccat ccgatctcgt cgaacgtcgc accgaggttg
      181 cccgccatct gcggcagcgc cacgttcacg atcgtggtgt cgagcacttc catgatcgcc
      241 gcggtgatca ggcctaacag aatcagccag cggtgtttgt tcagctccgc atcggcggcc
      301 tcgttcgccg ccacggagtc agccgtcgcc gccgaagcgc ccgatgtgcg tgttggtgca
      361 gtcacagccc agctcgtctc ctgtcccgtt catggtaccg ccatacgcca gcccggctcg
      421 cacggcgcac gcctggcgtc gtccgaaccg gggtccgtag aatctaaacg gaggagcggg
      481 gcgcgatgtg cccgcgttgt cgctacggcg ccggctccga gagcagttgg gacacgacgc
      541 gatcttcggt atcgtaggcc ccttcgccga aatggtggta acggatcttg ccgctcttgt
      601 cgatcaagaa cgcggcaggc cagtactgat tgctgaacgc cttccagatt gcgtagccgt
      661 tgtccatcac caccggatac gcgacgccca accgtttcac cgcatcgcgg acgttggcct
      721 cgtcggtttc cgttgggaac tcgggcgtgt gcacacccac caccacgaga ccgcgatcgc
      781 gatacttcct ctcgagcgcc tcgacgtgcg gcagggcgtt gaggcagttg tagcagagga
      841 acgtccagaa gttcaccatc acgactttgc cgcgcagcga cgccggtgtg agcggtgcgg
      901 agttgatcca cgcgccgccg cccgtgaatc cggggaacga tccttcgacg ccgagcggcg
      961 gcagcgacgg cggatcggcc ggcgtcgtac gcaccgcgac atcgcgcccg cccacgccgt
     1021 cgcccccgtg caacaacgcg accaaccgct gctcggcggg cgcggtcgcg ataccggcat
     1081 gcgcgaacac cgccgcgtcg cgccccgacg ccaacaggag caccaccgcg agcgtcccgg
     1141 cgcctaacgc tcgccgcgcc caacgctcgg cgccggcggc gccgcgcaac cgctcgagga
     1201 cgcgtccccc ggccgcgaga accacggcca gcgaggtcgc cgcgccggcc gcgaacgcga
     1261 ggtacaacga cgccgtgcgc acacccgctc cgctcgccgc agccgcggca atcacgaggc
     1321 cgagaatcgg accggcgcac ggcgcccaca acatcgcg
//
LOCUS       DASUCJ010000353         1322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1363992, whole genome shotgun sequence.
ACCESSION   DASUCJ010000353 DASUCJ010000000
VERSION     DASUCJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363992"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFU90_04245"
     CDS             <1..460
                     /locus_tag="VFU90_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEU5479016.1"
                     /translation="VQGRSAWPFCNASCPAVTWHVAAVATLSLHFAFIVFVVVGGLLA
                     WWWRWVAWLHVPAAIWGTWVELSGTVCPLTYVENAFRERAGEAGYREGFVAHYLLAAI
                     YPAGLTRETQYVLATFVVAINLIVYARLLRSARPRVAHGRRSGGRDGSRR"
     gene            432..974
                     /locus_tag="VFU90_04250"
     CDS             432..974
                     /locus_tag="VFU90_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479017.1"
                     /translation="MEVETVLDGDCTAEQRHRNEAEVGLFDREAAARLDDFTVPIHCN
                     LHRDFTSETVECDRYAHVSRGGCRSGCRGRHLGSIDCGERVGPPDRNFDERIPLDMQH
                     AFEHVGACPRDVGGRRLRGEMRCAPQFGLVDAHPVEPRVEISASCGDIDKSAYVTARD
                     DVVVAEACELATAVDDYGDE"
BASE COUNT          194 a          429 c          455 g          244 t
ORIGIN      
        1 ggttcaggga agatccgcgt ggccgttttg caacgcttct tgccctgccg tgacctggca
       61 cgtcgccgcc gtcgccacgc tgtcgctcca tttcgcgttc atcgtcttcg tcgtcgtcgg
      121 cgggctcctc gcctggtggt ggcggtgggt cgcatggctg cacgtacccg cggcgatatg
      181 gggtacctgg gtcgagctct ctggaaccgt gtgcccgctc acctacgtcg agaacgcgtt
      241 ccgcgagcgc gcgggtgagg ccggctaccg cgaaggcttc gttgcgcact acctgctcgc
      301 ggcgatttac ccagcgggac tgacgcgcga gacgcagtac gtgctcgcga cattcgtcgt
      361 cgcgatcaac ctgatcgtct atgcgcggct gctgcggtcg gcgcggcctc gcgtcgccca
      421 cggacgtcga agtggaggtc gagacggttc tcgacggtga ttgcaccgcc gaacagcgac
      481 atcggaacga ggccgaagtc ggtctgttcg atcgagaagc tgccgctcgc ctcgacgact
      541 tcaccgtgcc gatccactgc aatctgcacc gggacttcac gagtgagacc gttgagtgtg
      601 atcgatacgc gcatgtcagt cgcggcgggt gtcgcagcgg gtgtcgcggc cgccatctcg
      661 gcagtatcga ttgcggagag cgcgtcggcc cccctgaccg caacttcgac gaacggatac
      721 ctctcgacat gcagcacgcg ttcgagcatg ttggtgcgtg tccccgcgat gtcggcggcc
      781 gacggctgcg tggcgaaatg cgctgcgcgc cgcagttcgg gctcgtcgac gctcatccgg
      841 tcgagcctcg cgtagagatc agcgcgtcct gcggtgacat cgacaagtcc gcgtacgtca
      901 cggctcgcga cgacgtggtc gtggccgagg cgtgcgagct tgccaccgcg gtggacgatt
      961 acggtgacga gtgattgcgc gggatcgacg gcatacacgt cgtctccccg ctttcgggcg
     1021 atttcgtagt cggcggacgg aaacctggcc ggcgcgttct ccgatgtctg cggcgcgcgc
     1081 tcgggtggcg aagcgcctgt ggacagcggt gcgtgctcgg ccagcggtgc gcaggccgcg
     1141 aacagcatcg ccatgccgaa cacggtcgca cgcagcatcg caggggctac gagtcggagc
     1201 acgccgcggc aagttacgga tgttcggcgc acgctcaacg gccctcgtcg gggagtgcga
     1261 ccctgcgtga tcccgaatta cgcgcctcgc gctccatttc gtgcgcgagc gcgcgaacct
     1321 gc
//
LOCUS       DASUCJ010000354         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_752590, whole genome shotgun sequence.
ACCESSION   DASUCJ010000354 DASUCJ010000000
VERSION     DASUCJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752590"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /gene="trxA"
                     /locus_tag="VFU90_04255"
     CDS             complement(<1..288)
                     /gene="trxA"
                     /locus_tag="VFU90_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEU5479018.1"
                     /translation="MSESIKHVTDTSFEHDVLQAGNPVLVDFWAEWCGPCKMFAPVLD
                     EVARAYEGRLTVAKLDIDANPATPTKFGIRGIPTVILYKDGQAHAQKVGALT"
     gene            473..796
                     /gene="fdxA"
                     /locus_tag="VFU90_04260"
     CDS             473..796
                     /gene="fdxA"
                     /locus_tag="VFU90_04260"
                     /inference="COORDINATES: protein motif:HMM:NF045490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEU5479019.1"
                     /translation="MTYVVTESCIRCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECPVEAIYAEDDVPGAQQQFKALNAELARLWKPIIERTPAPADADQWKDVADKL
                     RYLER"
     gene            793..1254
                     /locus_tag="VFU90_04265"
     CDS             793..1254
                     /locus_tag="VFU90_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5479020.1"
                     /translation="MIAIGAVGFDRDARRKLELRGIPPVVTFPLIRQTVQRSMIRHAV
                     FAVALMTAAATCAAQTTAPVGDPARGQDKTRMCEGCHGLDGYRTAYPVVYSVPRLGGQ
                     HPEYLAKALHEYRAGERSHPSMRAIAASLSDQDIADLAAYYGASPKATASK"
     gene            1308..1586
                     /locus_tag="VFU90_04270"
     CDS             1308..1586
                     /locus_tag="VFU90_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5479021.1"
                     /translation="MPAAAADAAAGAVKVKEVCQACHGMNGNSTSPDYPRLGGQHADY
                     LRQALRDYKSGARKNPIMNGFAATLSEQDIENVAAYFASQPQVVFTKR"
BASE COUNT          290 a          620 c          534 g          293 t
ORIGIN      
        1 cgtcagcgcg ccgaccttct gggcgtgcgc ctggccgtcc ttgtacagga tgacggtcgg
       61 gatgccgcga atgccgaact tggtcggggt cgccgggttg gcgtcgatat cgagcttcgc
      121 cacggtcaac cggccctcgt acgcccgggc gacttcgtcg agtaccgggg cgaacatctt
      181 gcacggaccg caccactccg cccagaagtc gaccagcacc ggatttcccg cctgcagcac
      241 gtcatgctcg aacgaagtgt ccgtgacatg cttgatggat tcgctcattg ccgtccttgc
      301 ctcccacccg cttcacgcgg cacaccccgc agaagttggg gcccgagccg cgccggaagt
      361 ttcaatgata ccgcaagcac ctgtgtcgtc gcccggtgcc ggcgcaccat ctgctaccat
      421 gccgccttcg ccgcgaaggc gatgcatcgt cccgccaccg cgtcctcgca ccatgaccta
      481 cgtcgtcacc gaaagctgca ttcgctgcaa gtacaccgac tgtgtcgacg tgtgcccggt
      541 cgactgcttt cgggaaggcc cgaatttcct ggtgatcgac cccgacgaat gcatcgactg
      601 cacgctgtgc gtcgccgaat gtccggtcga ggcgatctac gccgaggacg atgttcccgg
      661 ggcgcagcag cagttcaagg cactcaatgc cgagctcgcc cgactctgga agccgatcat
      721 cgagcgcacg cccgcgccgg ccgacgccga tcagtggaag gacgttgcag acaagcttcg
      781 ttatctcgag cggtgatcgc aatcggcgct gtcgggtttg accgcgacgc ccgccgcaaa
      841 ctagaattgc gcggcattcc ccccgttgtc acattcccac tgattcgcca gaccgtccaa
      901 cgatcgatga tccgccatgc cgttttcgcc gtcgccctga tgacggctgc tgctacctgc
      961 gccgcgcaga cgacggcccc cgtgggcgac cccgcgcgcg gccaggacaa gacccgcatg
     1021 tgcgagggct gtcacggtct cgacggctac cgcaccgcct atccggtcgt ctacagcgtt
     1081 ccccgtctcg gcggacagca ccccgaatac ctcgccaaag ccttgcacga gtaccgcgcc
     1141 ggcgagcgca gccacccgtc gatgcgcgcg atcgcggcat cgctttccga ccaggacatc
     1201 gccgatctcg ccgcgtatta cggcgcgagc ccgaaggcga cggcgagcaa atgatgaaca
     1261 ctcttcgcgc tgcgctcctc cttgcggggc tcgcgatgct ggcgagcatg ccggccgcgg
     1321 ccgccgatgc cgcggcgggc gccgtcaagg tgaaggaagt ctgccaggcc tgccacggca
     1381 tgaacggcaa cagcacatcg cccgactatc ctcgcctcgg cggccagcac gccgactacc
     1441 tgaggcaggc gctgcgcgac tacaagtccg gtgcccgcaa gaatccgatc atgaacggct
     1501 tcgcggcgac gctctcggag caggacatcg agaacgtcgc ggcgtatttc gcgtcgcagc
     1561 cgcaggtcgt cttcaccaag cgctgattcg gcgctgccac ctgagttggt cgaaggagcg
     1621 tgcggcgtcg cgtcgcgcgc acgacgcgcg tcgtcctgcg tccgggtgag cctcggctca
     1681 gcgcttctcc ggccactcgc agaggtcggc gatgacgcat tcggcgcacc gcgggtt
//
LOCUS       DASUCJ010000355         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_859584, whole genome shotgun sequence.
ACCESSION   DASUCJ010000355 DASUCJ010000000
VERSION     DASUCJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859584"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFU90_04275"
     CDS             complement(<1..299)
                     /locus_tag="VFU90_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479022.1"
                     /translation="MQLRIPTPWKCDLVVRSAGDAIVESTFVVTRAKRVEKPGDALLA
                     AAAEQVRAYFARRLRRFDLPLDPAGTPFQRTVWELVAALSFGEFVSYADVARAAGR"
     gene            463..>1202
                     /locus_tag="VFU90_04280"
     CDS             463..>1202
                     /locus_tag="VFU90_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479023.1"
                     /translation="MTRTFVRTMALVAGFALAACQGGSYGSSAIPQGPGVPAQQSMPE
                     QPSDMPSAPGAQAPAEQAGPELPVAGDGEIPITAANAVHQVCPRPLPGQRQCFALERQ
                     DLRANDLNPNATHQGYGPSDLQSAYNIAGGRPSLVAIVDAYGYPKASSDLASYRSSYH
                     LPACTTGNGCLKIRNQTGGTRLPRSNQNWDAEQAVDLDMVSAMCPGCKLLLVEANSDY
                     TTDLYQAVSEAGKLGAVVISSSFGASEY"
BASE COUNT          210 a          401 c          410 g          181 t
ORIGIN      
        1 cgtccggccg cgcgcgccac gtcggcgtac gaaacgaatt cgccgaacga cagcgcggct
       61 acgagctccc aaaccgtccg ttgaaacggc gtaccggccg gatcgagcgg caggtcgaac
      121 cgccgcagcc ggcgggcaaa atacgcgcgg acctgttcgg cggcggcggc gagcaaggcg
      181 tcgccgggtt tttcgacgcg cttcgcgcgc gtaacgacaa acgtgctttc gacgatcgcg
      241 tcgccggccg agcgaacgac caggtcgcac ttccacgggg tcggaattcg cagctgcatc
      301 gaggggcgga ttcggcgtca gcaaccttcc ggtctaccgg ccgggcggat tccgggggcg
      361 ggtaggtgca caccccagct atatgcaaga gtggggcccg gccggccgga accggcggcg
      421 gttcgggtgc gttccctggt tgactagaat ttggaggctc ctttgacccg cacgttcgta
      481 cgcacgatgg cactcgtcgc cggtttcgcg ctggccgcct gccaaggcgg aagctacggt
      541 tccagcgcga tcccgcaagg ccccggcgtc ccggcccagc agtcgatgcc cgagcagccg
      601 tcggatatgc cgtccgcacc aggcgcgcag gcgccggccg agcaggccgg tcccgagctg
      661 ccggtcgccg gcgacggcga gattccgatc acggccgcca acgcggtgca ccaagtgtgt
      721 ccgcgtccgc tgcccggcca gcggcagtgc ttcgccctcg aacgccaaga tctgcgcgcc
      781 aacgatctca acccgaacgc gacgcaccaa gggtacgggc cgtccgatct gcagagcgcc
      841 tacaacatcg cgggcggccg gccgagtttg gtcgcgatcg tcgacgcgta cggctatccg
      901 aaagcctcga gcgatttggc gagctaccgt tcgtcctatc acttgccggc gtgcaccacc
      961 ggcaacggct gtctgaagat tcgcaatcaa acgggcggca cgcgacttcc gcgatcgaat
     1021 caaaactggg acgcggagca ggcggtcgac ctcgacatgg tttcggcgat gtgcccggga
     1081 tgcaagctgc tgctggtcga ggcgaactcg gattacacga cggatctcta tcaggcggtg
     1141 agcgaagccg gcaagctcgg cgcggtggtg atcagcagct cgttcggcgc gagcgaatac
     1201 ag
//
LOCUS       DASUCJ010000356         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1241866, whole genome shotgun sequence.
ACCESSION   DASUCJ010000356 DASUCJ010000000
VERSION     DASUCJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFU90_04285"
     CDS             <1..1056
                     /locus_tag="VFU90_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479024.1"
                     /translation="ASGNVHAVLPSGTTLDGPHVDYYRVAPNIRTVTKMIATGRPTIT
                     IIERDSTGKPSQPMTVVANTVDMEGDSLTYASMNVVITRTDVIATGDSAAMNSATEFA
                     RLIRKPMIKARGDRPFTLTGDLIDLFGRNKAVDRVISRGHARSVSKDATLTGDTLDFA
                     MAQGRLQRVHAWGTSRAHAFNPTYDMLADSLDVRMPDQRLREIHALRDAYAQSVPDTT
                     KIHTSEHDWLRGDTIYAYFDSSAKALADTSTQPQIQRLISFGHARSFYQIAAKDTAAI
                     GPSINYVRGDRIQVAFLNREVQTVTIKGQATGVYLDPSKPPRDTTKKGKTDTATVKTP
                     NAAPKTRADTARGATPR"
BASE COUNT          241 a          396 c          349 g          165 t
ORIGIN      
        1 gcctcgggca acgtgcacgc cgtgctcccg agcggcacta cgttggacgg accgcacgtc
       61 gattactatc gcgtggcgcc gaacatccgc accgtcacca aaatgatcgc gacgggccgc
      121 ccgacgatca cgatcatcga gcgcgactcc acggggaagc cgtcgcaacc gatgaccgtc
      181 gtcgccaaca ccgtcgatat ggaaggcgac agcctgacgt acgcgtcgat gaatgtggtc
      241 atcacgcgca cggacgtcat cgccacgggc gattcggccg cgatgaacag tgccaccgag
      301 ttcgcgcgcc tcattcgcaa gcccatgatc aaggcgcgcg gcgaccgtcc gttcacgctc
      361 accggcgatc tcatcgatct cttcggccgc aacaaggcag tcgatcgcgt gatctcgcgc
      421 ggccacgcca ggagcgtgag caaggatgcc acgctcaccg gcgacacact cgacttcgcg
      481 atggcccagg gccggctgca gcgcgtgcac gcgtggggca cgagccgcgc gcatgcattc
      541 aatccgacgt acgacatgct ggccgattcg ctcgacgtgc gcatgccgga ccaacgcctg
      601 cgcgagatcc atgcgctgcg cgatgcgtat gcgcagagcg tgcccgacac gaccaagatc
      661 cacacgagcg agcacgactg gctgcgcggc gacaccattt acgcctactt cgacagctcg
      721 gccaaggcgc tcgccgacac gtccacacag ccgcagatcc agcggctcat ctcgttcggc
      781 cacgcgcggt ccttttatca aattgcggcg aaggacaccg cggccatcgg gccgtcgatc
      841 aactatgtgc gcggtgatcg catccaagtc gcgttcctga accgcgaagt gcaaacggtc
      901 acgatcaagg ggcaggcgac gggcgtctat ctcgatccga gcaaaccccc gcgcgacacg
      961 accaagaagg gcaagaccga taccgcgacg gtcaagacgc ctaacgcagc accgaaaacg
     1021 cgcgccgaca ccgcccgggg agccacaccg cgatgaccga cccgattcgt ttgcccgaag
     1081 aggtcgagaa cttcatcgaa gagaagtcgc gcggcgaccg ttcggtggcg ttggcgatga
     1141 aagcgttggt c
//
LOCUS       DASUCJ010000357         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_599720, whole genome shotgun sequence.
ACCESSION   DASUCJ010000357 DASUCJ010000000
VERSION     DASUCJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599720"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1399)
                     /locus_tag="VFU90_04290"
     CDS             complement(56..1399)
                     /locus_tag="VFU90_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479025.1"
                     /translation="MALALSAWLLAGCASAGNGAPGATVPQPGAGRPGTASGKIAHVV
                     VIVQENRSFNNLFQGFPGADTQPYGYTSSGKKVALRPVGLEADWDIVHSSKAYFDACD
                     GSGSLPGTGCKMDGFDKEPFVCSRGLHPYCPPRDSQYAYVPNAETKPYFAIAKQYVLA
                     DRAFTSNFDGSSFVSHQYFIAGQANAAVDYPSQPYWGCDGGKGDTVVTLTQARTYGPP
                     IRACFDDRTLANEMDAAGVSWRYYTSGIRQDSYIWNAFQAIRPVRYGPEWKTNVVTPQ
                     SRFFKDVQNGALPAVSWITPTCANSDHSSCGSNHGPAWVASLVNAVGESPYWNSTVVF
                     VMWDDYGGWYDHVPPPLADYDGLGIRVPLLVVSAYAKAGYVSHVQYEHGSVLKFIEDQ
                     FGLAPLAASDARANSPAADCFDFNQPPRAFKKIRAPLGPADFGREPLDPRPIDDE"
     gene            complement(1396..>1579)
                     /locus_tag="VFU90_04295"
     CDS             complement(1396..>1579)
                     /locus_tag="VFU90_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479026.1"
                     /translation="FVEDQFGLAPLAASDARANSPEPYCSNFTKPPRAFKTIPAPQQE
                     PYFLRQPSDSRPVDDE"
BASE COUNT          274 a          498 c          524 g          283 t
ORIGIN      
        1 caggatgaca cacattgtgc ggcgctccgc tcaggatgac acacattgtg cggcgctatt
       61 cgtcgtcgat cgggcgcggg tcgagcggct cgcgcccgaa atcggccggc ccgagcggtg
      121 ctcggatctt cttaaaagcg cggggcggct ggttgaagtc gaagcagtcg gccgccggcg
      181 agttcgcgcg cgcgtccgaa gccgccagcg gcgcgagtcc gaattgatcc tcgatgaatt
      241 tcagcacgct gccgtgttcg tattgcacgt gcgacacgta ccccgccttt gcgtaggcgg
      301 aaacgaccag cagcggcaca cgaatcccta agccgtcgta gtcggccagc ggcggcggca
      361 cgtggtcgta ccagccgccg tagtcgtccc acatcacgaa gacgacggtc gaattccagt
      421 aagggctctc gccgaccgcg ttcacgagcg atgccaccca ggccggtccg tgattcgagc
      481 cgcagctcga gtgatccgag ttggcgcagg tcggcgtgat ccagctcacc gccggaagcg
      541 ccccgttctg cacgtccttg aagaaccggg attgcggcgt gacgacgttg gttttccact
      601 cggggccgta acgcacgggc cggatggcct gaaaggcgtt ccagatgtag ctgtcctgcc
      661 gaatccccga ggtatagtag cgccacgaaa ccccggccgc gtccatctcg ttcgcgagcg
      721 ttcgatcgtc gaagcaagca cgaatcggcg gaccgtaggt gcgcgcttgg gtgagggtta
      781 cgacggtgtc gcctttcccc ccgtcgcatc cccaatacgg ttggctcggg tagtcgaccg
      841 ccgcattggc ttggcccgca atgaagtact ggtgcgacac gaagctgctg ccgtcgaagt
      901 tcgacgtgaa cgcgcggtcg gcgagcacgt attgcttggc gatggcgaaa tacggtttgg
      961 tctccgcgtt cgggacgtag gcgtactgcg aatcgcgcgg cgggcagtac ggatgtaagc
     1021 cgcggctgca cacgaacggc tccttgtcga agccgtccat tttgcagccg gttcccggta
     1081 aactacccga tccgtcgcac gcgtcgaagt acgccttcga cgagtgaacg atgtcccaat
     1141 cggcctccag cccgaccggg cgcaacgcga ccttcttccc cgaactggta tacccgtacg
     1201 gctgcgtgtc ggctcccgga aaaccttgga agaggttgtt gaagctgcga ttctcttgga
     1261 cgatcacgac gacgtgggcg attttaccgc tcgcggtgcc gggccggccc gcgcccggct
     1321 gcggaacggt cgcgcccggt gcgccgttac cggcgctcgc gcagcccgcg agaagccagg
     1381 ctgaaagtgc gagcgctatt cgtcgtcgac cggacgcgaa tcggacggtt gccgcaggaa
     1441 gtacggctcc tgctggggcg cgggaatcgt cttgaaggcc cgcggcggtt tggtgaagtt
     1501 ggagcaatac ggctccggag agttcgcccg cgcgtccgat gcggccagag gcgccagccc
     1561 gaactgatct tcgacgaac
//
LOCUS       DASUCJ010000358         5884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_294129, whole genome shotgun sequence.
ACCESSION   DASUCJ010000358 DASUCJ010000000
VERSION     DASUCJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294129"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFU90_04300"
     CDS             complement(<1..459)
                     /locus_tag="VFU90_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479027.1"
                     /translation="MFAAVKRHASGWKGAIVAYAAVSLLALAGLLLTPGTLGHHWDWL
                     IPADPAELRRLAEIQAYSWQDYAFGSYVTYRYATVLTGLLFGLPGFLGLSGAFVSKTL
                     VLASVFVSGLAMRFALLSLTHDDPDERDGAFATFGGFLYALAPYAYNQIVA"
     gene            519..1058
                     /locus_tag="VFU90_04305"
     CDS             519..1058
                     /locus_tag="VFU90_04305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5479028.1"
                     /translation="MIVLCRHGATDGNLAGAFLSSGDPPLNALGRTQSERLRAALFAM
                     KFGVAFASPMRRCVETLAIVAPDVPSSVREELREIGFGEWEGRTVEWLEIHDPDGVAR
                     RRSDPASFRPAGGESFLDVARRLHPFVEALRSRTAGDALVVAHRGTLGVLERLLRGLP
                     TGSREVTPLELGEYRLLTE"
     gene            complement(1064..2050)
                     /locus_tag="VFU90_04310"
     CDS             complement(1064..2050)
                     /locus_tag="VFU90_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5479029.1"
                     /translation="MRILISAGGTVTAQSLIKALREDGRASFVAIVDRDDLNATKAFV
                     DAFERVPSADDPEFVPACLDAVRRHRIDLFVPIIVEREFLPLIAHRGRFEESGCRVAV
                     PAREIVLNAGDKLAFAAFLESLGIDGPKTVAYTPDGPTVGFPAYLKPRRGSGSVGTQR
                     VESAPMLHAAARGRTDLIVQEAVDGTEFTVDCFAAEAGRVVAAVPRERIAIKAGVSVK
                     GRTYRHPQIEAIASDVVARTGIAGPANVQGMLRADGSFSIIEMNPRFSGTLALTTAAG
                     VNFASLLLDQIEGKPIADMRGRHRAGVTMLRYWSEAFEGPDGSAWLGTSMSR"
     gene            complement(2060..3061)
                     /locus_tag="VFU90_04315"
     CDS             complement(2060..3061)
                     /locus_tag="VFU90_04315"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5479030.1"
                     /translation="MASPKAVLITGGAGFIGSHVVEDLLASGHRVTVFDNFSSGSREN
                     LASIAGDVEIVTGDVLDRDAIVAAARGKDVVSHQAAQLEITKCMEDPIGDLRTNLIGT
                     INVLEAARQAGVERVINASSACIYGQAVARPADEDAHPHDPNWSYGASKLAAEKYAGV
                     FSNDYGFPAFSLRYGIVYGPREWYGRVLTIFLKRALEGKGPVVFGEGEQLRDFVYVDD
                     VVAVHHACMESALAGPHSFNAATGIATSIAELAALVCEVTGIDASPVREEVEPGQRST
                     LVDGRMRLPAELAVMHLSPAKTERLLGVRAKTSLRDGIAREWSWLLEHPQRWNEMHY"
     gene            3321..4442
                     /locus_tag="VFU90_04320"
     CDS             3321..4442
                     /locus_tag="VFU90_04320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5479031.1"
                     /translation="MHVPFVDLKAQYASIKPQVRESIDEVLDAASFIMGPQVRRFEEA
                     FARYVGARHCVGVESGTGALKVALEALDIGPGDEVILPANTYVASAMAVSATGALPVL
                     VDCDDAYLIDTAAVEAAITPRTKAVMPVHLYGQAVPMQPLLDLARRHGLRVIEDACQA
                     HGAYWRGRRVGSVGDVGCFSFYPGKNLGAYGDAGAVVTGDDAIAERVRLLRDFGQQRK
                     YEHLIRGDNCRLDSIQAAVLAVKLEHLDDWNARRLAHARRYDALLAQAGFPVPRRLAD
                     DGHVYHLYVTQVPERAAVAEELARRGVQTGIHYPIPIHLQPAYRDLGYERGRFPNAEA
                     AAERILSLPMFAELTGEQIEYVVAALVEATRPLRHPARA"
     gene            4454..5479
                     /locus_tag="VFU90_04325"
     CDS             4454..5479
                     /locus_tag="VFU90_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5479032.1"
                     /translation="MTPSRELTAAIVGLGYWGPNLLRNFTTSTKWNVGWIVDTDERRR
                     SGLARLYPNARATADLDVVLGDAAVDLVAIATPPVLHFPMASRAIAAGKHVLVEKPLA
                     EDAGHARALVEAAERAGVRLFTDHTFLYAGAVEAMRRSLTGGELGEIYYAESTRVNLG
                     LFQQTHVIWDLAPHDLSILNHVFGELPHSVACHAMAYIHPPVWDVAFLLLRYGSGRIA
                     QVHLSWLSPVKVRRMMLSASTRMVVWDDVDPSEKLRVYDKGVVRNPTSETLSQQMVSY
                     RLGDVRMPIVDGREPLAKMADDLYDSISGGAPSRIGGAVGYDVVRCIEAACKSAELNG
                     AWVNVEP"
     gene            complement(5448..>5884)
                     /locus_tag="VFU90_04330"
     CDS             complement(5448..>5884)
                     /locus_tag="VFU90_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479033.1"
                     /translation="GSRLRSESEGVEVRGSWKPYTLGRAGAPAALAPAAGDVSAVASR
                     PCPALTAAPEVPKTAFVFASGYDAGWRARAGGRFVAPMLANGWMMAWDAAAASQPLAY
                     LPAYLQAAGFAIAVVVLAGAFGLARRADARAAITAPRSPTHR"
BASE COUNT          979 a         2031 c         1923 g          951 t
ORIGIN      
        1 cgcgacgatt tggttgtagg cgtaaggcgc gagcgcgtaa aggaatccgc cgaacgtcgc
       61 gaaggctccg tcgcgttcgt cgggatcgtc gtgcgtgagc gagagcagcg cgaagcgcat
      121 ggccagcccg gagacgaaaa cgctcgccag cacgagcgtc ttgctcacga aggcgccgct
      181 caggccgaga aatcccggca atccgaacag caaaccggtc agcacggtcg cgtagcgata
      241 cgtgacgtaa gagccgaagg cgtaatcttg ccacgagtag gcttggatct cggcgagccg
      301 ccgaagctcc gcgggatccg ccgggatcag ccagtcccag tggtgtccga gcgttcccgg
      361 cgtcagcagc agtccggcga gcgcaagcag cgaaacggcg gcatacgcga cgatcgctcc
      421 cttccatccg gacgcatgac gcttaacggc agcgaacatc gccccgctcg ttggccacgg
      481 acggagggct tccctcctcg cgccgaaatg cgtacgcgat gatcgtcttg tgcaggcacg
      541 gcgcaaccga cggcaacctc gccggcgcgt tcctttccag cggcgatcca ccgctcaacg
      601 cactcggacg cacgcaaagc gagcggcttc gtgcggcgtt gttcgcaatg aagttcggcg
      661 tagctttcgc gagcccgatg cgccgctgcg tcgaaacgct cgcgatcgtc gcgccggacg
      721 ttccttcgag cgttcgcgaa gaactgcgcg agatcggttt cggtgagtgg gaagggcgca
      781 ccgtcgagtg gctcgagatc cacgatccgg acggcgtcgc gcgccgccgt tccgatccgg
      841 cgagcttccg tccggccggc ggcgagagct ttctcgacgt cgcgcggcgc ctgcatccgt
      901 tcgtcgaagc gctgcgttcg cgtacggccg gcgacgcgct cgtcgtcgcg caccgcggca
      961 cgctgggagt cctcgaacgg ctgctgcgcg gcttacccac cggctcgcgc gaggtgacgc
     1021 cgctcgaact cggcgagtac cggttactca cggagtagac tcgctagcgc gacatcgacg
     1081 tgccgagcca cgcgctgccg tcggggcctt cgaaggcctc gctccagtag cgcagcatcg
     1141 tcacgcctgc ccgatggcgc ccgcgcatgt cggcaattgg tttgccctcg atttgatcga
     1201 gcagcagcga tgcgaagttc acgccggcgg cggtggtcaa cgccaacgtc cccgaaaacc
     1261 gcggattcat ctcgatgatc gaaaaggaac cgtcggcacg cagcatgccc tgcacgttgg
     1321 cggggccggc gatgccggtg cgcgcgacga cgtcgctcgc gatcgcttcg atctgcgggt
     1381 ggcggtacgt ccggcctttc acggagacgc ccgctttgat cgcgatgcgc tcgcgcggta
     1441 ccgccgccac gacgcgtccc gcttcggcgg cgaagcaatc gaccgtaaat tccgttccgt
     1501 cgacggcttc ttgtacgatc aagtcggttc ggccgcgcgc cgccgcgtgc agcatcggcg
     1561 cgctctcgac gcgttgcgtc cccaccgaac cggatccgcg acgcggcttg aggtaggccg
     1621 gaaatcctac cgtcgggccg tcgggcgtgt aggcaacggt cttggggccg tcgatgccga
     1681 ggctttccag gaaggcggcg aaggcgagct tgtcgccggc gttgagcacg atctcgcgcg
     1741 ccggtaccgc gacccggcag cccgactcct cgaatcgccc gcggtgcgcg atcaacggca
     1801 gaaattcgcg ttccacgatg atcggaacga agagatcgat ccgatgccgc cgcacggcat
     1861 ccaagcacgc cggaacgaat tcggggtcgt cggccgaggg aacgcgctcg aatgcgtcga
     1921 cgaacgcctt ggtggcgttg agatcgtcgc gatcgacgat tgcgacgaac gacgcacggc
     1981 cgtcctcgcg cagtgccttt atcaacgact gcgcggtcac cgttccgccg gccgagatga
     2041 gtatcctcat tggggttcct taatagtgca tttcgttcca gcgctgcgga tgttcgagca
     2101 gccagctcca ctcgcgcgcg atcccgtcgc gcagcgaggt tttggcgcgg actcccagca
     2161 gccgttcggt tttggccggc gacaaatgca tgacggccaa ctcggcgggc aaccgcatgc
     2221 gtccgtccac cagcgtcgac cgttggcccg gctcgacctc ctcgcgcacc ggcgacgcgt
     2281 cgatccccgt cacctcgcac acgagcgcgg ccagctcggc gatcgaggtc gcgatcccgg
     2341 ttgcggcgtt gaacgaatgc gggccggcga gcgcgctctc catgcaggcg tggtggacgg
     2401 cgacgacgtc gtcgacgtag acgaaatcgc ggagctgctc tccttcgccg aagacgaccg
     2461 ggcctttccc ttcgagcgcg cgcttgagga aaatcgtcag cacccgcccg taccactcgc
     2521 gcggcccgta cacgatgccg tagcgcagcg agaacgccgg aaaaccgtag tcgttggaaa
     2581 acactcccgc gtatttctcg gccgcgagct tgctcgcgcc gtacgaccag tttggatcgt
     2641 gaggatgtgc gtcttcgtcg gcggggcgcg ccaccgcctg gccgtaaatg caggcggacg
     2701 acgcattgat cacgcgctcg acgcccgctt ggcgcgccgc ctcgagcacg ttgatcgtcc
     2761 cgatcaaatt cgttcgcaga tcgccgatcg gatcttccat gcatttggtg atctcgagct
     2821 gcgccgcctg atgcgaaacg acgtccttgc cgcgcgccgc cgcgacgatc gcgtcgcgat
     2881 cgagaacgtc gccggttacg atctcgacgt cgcccgcaat cgatgctaag ttctcgcgcg
     2941 atccgctcga gaagttgtcg aacacggtga cgcgatgtcc gctcgcgagc aggtcttcga
     3001 cgacgtgcga gccgatgaac cccgcgccgc cggtgatcag gaccgccttt ggggaagcca
     3061 ctatctcctc cgtcaaaaaa aagcgctccg agcgccgggc cgcgcgcgag cgaatgcacg
     3121 ccgtaaacgc gaacgaaccc tcgctcgcgg cgaggctgta ttgaacctcg gcaccgcaaa
     3181 ctccgctcga accgctctcg cccgggctgc cggaagagtc gcagcgcggg cacccgcgca
     3241 tggatagcca aggacgtccc cgaaaagggg gcgcggacct cgagcaccct cgcaatccct
     3301 tcaggagccg atcgcccgcc atgcacgtcc cgttcgtcga tctcaaagcg cagtacgcct
     3361 cgataaagcc gcaagttcgg gaaagcatcg acgaagtgct cgacgcggcc agcttcatta
     3421 tgggaccgca agttcgccgg ttcgaggagg cgttcgcacg ctacgtgggc gcccgtcact
     3481 gcgtcggcgt cgaatcgggg accggcgctc tgaaggtcgc gctcgaagcg ctcgacatcg
     3541 gccccggcga cgaagtgatc cttcccgcca acacctacgt cgcgtcggcg atggccgtct
     3601 cggccaccgg cgcgttgccg gttctggtcg attgcgacga cgcctatctc atcgataccg
     3661 ccgccgtcga agccgccatc acgccgcgta cgaaggccgt tatgccggtt cacctctacg
     3721 ggcaggcggt gccgatgcag ccgcttctcg acctcgcgcg gcggcacgga ctgcgcgtca
     3781 tcgaggacgc atgtcaagcg cacggcgcgt actggcgagg gcgtcgcgtc ggaagcgtcg
     3841 gcgacgtcgg gtgtttcagt ttttatcccg gcaagaacct cggcgcgtac ggcgacgccg
     3901 gcgcggtcgt caccggcgac gacgcgatcg ccgagcgcgt tcggctgctg cgcgatttcg
     3961 ggcaacagcg taagtacgaa catctgatca ggggcgacaa ctgccggctc gattcgatcc
     4021 aagcggcggt gctcgccgtc aagctcgagc atctcgacga ctggaacgct cgccggctcg
     4081 cgcacgcgcg ccgctacgac gcgctgctcg cacaggccgg cttcccggtc ccgcggcggc
     4141 tcgccgacga cggacacgtc taccatctct acgtgacgca ggtccccgag cgtgccgccg
     4201 tcgccgaaga gctggcgcgg cgcggcgttc aaaccggcat tcactacccg atcccgattc
     4261 atctgcagcc ggcgtatcgc gatctcggct acgagcgcgg ccggtttccc aacgccgaag
     4321 cggcggccga acggattctc tcgctcccga tgtttgcgga gctgaccggc gagcagatcg
     4381 agtacgtcgt cgccgcgctc gtcgaggcga cgcgcccgct gcgccacccg gcccgagcgt
     4441 agggctcttc ccggtgactc cgtcgcgcga gcttaccgcg gcgatcgtcg gcctcgggta
     4501 ctggggcccg aacttgctgc gcaatttcac gacctcgacg aaatggaacg tcggctggat
     4561 cgtcgacacc gacgaacgcc ggcgaagcgg cttggcgcgg ctctacccca acgcgcgcgc
     4621 gaccgccgat ctcgacgtgg tgctcggcga cgccgcggtc gacttggtcg cgatcgcgac
     4681 gccgccggtt ctgcacttcc cgatggcgtc gcgggcgatc gcggccggaa aacacgtcct
     4741 cgtcgaaaag ccgctggcgg aggacgccgg tcacgcgcgc gcgctcgtgg aagccgccga
     4801 acgcgccggc gttcggctgt ttaccgacca cacgtttctc tacgccggag ccgtcgaagc
     4861 gatgcgccgc agcctcaccg gcggcgagct cggcgagatc tattacgccg aatcgacgcg
     4921 cgtcaatctc gggctgttcc agcagaccca cgtgatctgg gatctcgcgc cgcacgacct
     4981 ctcgattctc aatcacgtgt tcggcgagct tccgcactcg gtcgcctgtc acgcaatggc
     5041 gtacattcat ccgcccgtct gggacgtggc cttcttgctg ttgcgctacg gcagcgggcg
     5101 catcgcccaa gttcacctga gctggctttc gccggtgaag gtgcgccgca tgatgctttc
     5161 ggcgagcacc cgtatggtcg tttgggacga cgtcgatcca tccgaaaagc tgcgcgtcta
     5221 cgataagggc gtcgtgcgca atcccaccag cgaaacgctg tcgcagcaga tggtcagtta
     5281 ccggctgggc gacgttcgca tgccgatcgt cgacggccgc gaaccgctgg ccaaaatggc
     5341 cgacgacctc tacgacagca tcagcggcgg agcgccctcg cgaatcggcg gtgcggtcgg
     5401 ttacgacgtc gtgcgctgca tcgaagccgc ctgcaagtcc gccgagctca acggtgcgtg
     5461 ggtgaacgtg gagccgtgat cgccgcgcgt gcgtcggcgc gcctcgccag cccgaaggca
     5521 cccgccagca cgacgaccgc gatcgcgaag ccggccgcct gcaaataggc cggcaggtag
     5581 gccagcggct gggatgccgc cgctgcatcc caagccatca tccagccgtt ggcgagcatc
     5641 ggcgcgacga aacggccgcc ggcgcgcgcg cgccagcccg cgtcgtaacc gctggcgaac
     5701 acgaaggcgg tcttcggtac ttccggcgct gccgtcaaag ccgggcaggg gcgcgaagcg
     5761 accgcgctca cgtcgccggc cgcgggcgcg agcgctgcgg gcgcgccggc gcgcccgagc
     5821 gtatacggtt tccaggaacc ccgcacctca acgccttccg attcgctccg cagtcgagat
     5881 ccgc
//
LOCUS       DASUCJ010000359         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_691360, whole genome shotgun sequence.
ACCESSION   DASUCJ010000359 DASUCJ010000000
VERSION     DASUCJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691360"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..2530
                     /locus_tag="VFU90_04335"
     CDS             338..2530
                     /locus_tag="VFU90_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5479034.1"
                     /translation="MPRYRFAARRLRAPRRAARSATSLGLAIAVLGAAVPAAAQMPNE
                     RLQYPAAHRDSTVDDYFGARVPAPYRWMEDENSPEVRSWVEEENAVTAAYMDRVPLRD
                     AFKKRLTDLWNYEKAGVPYRQAGRLFYSKNSGLQNQSVLYEQDRLGSAPRAMLDPNTL
                     SPDGSTALAGSSVSPNGEYLAYGLSQGGSDFEELHVRSLRDGRDTPDTVRWVKFSGIS
                     WTADNRGFFYSRFPAPAAGQALSTAAVDQKVYYHVLGTPDSTDKLIYARPDLPDWYIM
                     SSTTEDGRFAFITLVHGTDTKNQVFYMDLKDGAHPDLSAPLLPLVSQGDAEYAPLGND
                     GDTVFVQTTNQAPNRRVIAMVLPDTSRAHWRTVVPEQKSVLERATLAGGHIVAQYLED
                     VKSTLAIYGTDGSKQGAVALPGIGTVGGLSGRMDTPELFYSFSSFLFPATVYRYDFAT
                     KRSVPFQAPHVAFDASAYETRQVFYRSKDGTRVPMFITARKGLKLDGSHPTVLYAYGG
                     FDISVTPSFSPTTAVWLEHGGIYAVASLRGGGEYGEAWHHAGMLDNKQNVFDDFIAAA
                     EYLIHEHYTSTAHLAIHGYSNGGLLVGAVLDQRPDLFAAAYPGAGVMDMLRYQKFSAG
                     VGWVPEYGSSDDSTQFKYLIKYSPVQNLKKGTCYPATIVTTADHDDRVVPSHSYKFIA
                     TMQADQSCARPVLIRVETKTSHGYMPTDKRIAQLADVWAFTGWNTGMR"
     gene            2541..>3203
                     /locus_tag="VFU90_04340"
     CDS             2541..>3203
                     /locus_tag="VFU90_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479035.1"
                     /translation="MRVSGIGIGPSPNDAGDVRLEAHVTFDDASTPESVWIDVPAAYA
                     DSVSPNGNPWLACLMPLAMQRREPLHVDAPVDGALLDGMNEVARLWTAWFPPFTPVPV
                     TVASVLGPPDGGGPGTRLGAFFSGGVDSFFTLLRREGERPRNPHLAVDDLIVVHGFDV
                     PLSDGAAFDRLRTSLQRVADELGKTLIPVRTNIRVTRFRELEWARLGHGPALAAIALA
                     LEP"
BASE COUNT          526 a         1105 c         1038 g          534 t
ORIGIN      
        1 cgagcagccg agcgatccgt gccgttctcc gtgctgtcat ccgttcaatc gttcaatccg
       61 tgtctccaga gcgaggagac cgaccacctc gcatcttttc cggcacgggc cgcgcgtccg
      121 ctcgccaaaa tgaaccggtg ccagaaccgg ggtagaaata ccgtttggtc cggtaccgcg
      181 ctgagactcg ttgatcgcga cccgagtggc gtgatacacc cttccgactc acgcgtgagt
      241 cgccggtttg caccggctgt ggccggcgcc tactttccgg acgtccgagt cgatcggcgc
      301 gcgtgtgcgc gcccccgtca cgccacggag agtctggatg ccccgctacc gctttgcggc
      361 ccggcgcctg cgcgcccccc gccgcgctgc ccgttccgcc acatcgttag gcctggcgat
      421 cgccgtcctc ggcgccgccg tgcctgccgc cgcgcagatg cccaacgagc ggctgcagta
      481 ccccgccgcg catcgcgaca gcacggtcga cgactatttc ggcgcgcggg tgccggcgcc
      541 ataccggtgg atggaggacg agaacagccc ggaggtgcgc agctgggtgg aggaagagaa
      601 cgccgtgacg gccgcgtaca tggacagggt gccgctccgc gatgcgttca agaagcgcct
      661 aactgatctc tggaactacg agaaggccgg tgtgccgtac cggcaggccg gccgcctgtt
      721 ctacagcaag aactccggcc tccagaacca gagcgtgctg tacgagcagg accgcctggg
      781 gagcgcgccg cgcgcgatgc tcgaccccaa cacgctctcg cccgacgggt cgacggcact
      841 cgccggctcg tcggtctcgc ctaacggtga gtatctcgcg tacgggctgt cgcaaggcgg
      901 ctcggacttc gaggagctcc atgtgcgctc gctgcgcgac ggccgcgaca ccccggacac
      961 cgtgcggtgg gtcaagttct ccggcatctc gtggaccgcc gataaccgcg gcttcttcta
     1021 ttcgcggttt ccggcgcccg cggccgggca ggcgctgtcc acggccgccg tcgatcagaa
     1081 agtgtactac cacgtgctcg gcacacccga cagcaccgac aagctgattt acgcgcggcc
     1141 ggacctgccc gactggtaca tcatgtcgtc gaccaccgaa gacggacgct tcgcgttcat
     1201 cacgctcgtc cacggcacgg atacgaagaa ccaggtcttc tacatggatc tgaaggatgg
     1261 cgcccatccg gatctgtcgg cgccgctcct gccgctcgtc tcacagggcg atgccgaata
     1321 cgctccgtta ggcaacgacg gcgacacggt gttcgtgcag accaccaacc aggccccgaa
     1381 ccgccgcgtc atcgcgatgg tgctgcccga tacgtcgcgc gcgcattggc gcactgtggt
     1441 ccccgaacag aagagcgtcc tcgagcgcgc aacgctggcc ggcggacaca tcgtggccca
     1501 atacctcgag gacgtgaaga gcacgctcgc gatctacggt accgacggct cgaagcaggg
     1561 cgccgtcgcg ctgccgggca tcggcaccgt cggcggtctc tcggggcgca tggatacgcc
     1621 cgagctcttc tacagctttt cgtcgttcct cttcccggca accgtctatc gctacgactt
     1681 cgcgacgaag cgcagcgtgc cgtttcaagc gccgcacgtc gccttcgacg cgagcgcata
     1741 cgagacgcgc caggtgttct atcggtccaa ggacggcacg cgcgtgccga tgttcatcac
     1801 ggccagaaaa ggtctcaagc tcgatggctc gcacccgacc gtgctctacg cctacggcgg
     1861 attcgacatc agcgtgacgc catcgttcag tccgaccacg gccgtgtggc tggagcacgg
     1921 cggcatctac gcagtggcca gccttcgcgg cggcggcgaa tacggcgagg catggcatca
     1981 cgcgggcatg ctcgacaaca agcagaacgt attcgacgac ttcatcgcgg cggccgagta
     2041 tctcatccac gagcactaca cgtccacggc gcacctcgcc atccacggct actccaatgg
     2101 cggcctgctc gtgggcgcgg tgttggacca acgcccggac ctgttcgccg ccgcgtatcc
     2161 gggcgccggc gtgatggaca tgctgcgcta tcagaagttc agcgccggcg tgggttgggt
     2221 gcccgaatac ggctcgtcgg atgactcgac gcagttcaag tatctgatca agtattcgcc
     2281 ggtgcagaat ctcaagaagg ggacgtgtta tccggcgacg atcgtcacca ccgccgacca
     2341 cgacgaccgc gtggtgccga gccactcgta caagttcatc gccacgatgc aggcggatca
     2401 gtcgtgcgcg cggcccgtgc tcatccgtgt cgagacgaag accagccacg gctacatgcc
     2461 cacggacaag cggatcgcac agctggcaga tgtgtgggcg ttcacgggct ggaatacggg
     2521 catgcggtga cgaccgaagc gtgcgcgtaa gcggaatcgg catcgggccg tcgcccaacg
     2581 acgccggcga cgtgcgcctc gaggcgcacg tgacgttcga cgacgcgtcg acgccggagt
     2641 cggtgtggat cgacgtgccg gccgcgtacg ccgattcggt ctcgcctaac ggaaatccgt
     2701 ggctggcgtg tctcatgccg ctggccatgc agcgccgcga accgctgcac gtcgacgcgc
     2761 cggtcgacgg cgcgctgctc gatggcatga acgaagtggc gcgtctctgg accgcgtggt
     2821 tcccgccgtt cacgcccgtg cctgtgaccg tcgcgtcggt gctcgggccg ccggatggcg
     2881 gcgggccggg cactcggtta ggcgcgttct tttccggcgg cgtcgattcg ttcttcacgc
     2941 tgctgcgccg cgaaggcgaa cggccgcgca atccccacct cgccgtggat gacctcatcg
     3001 tcgtgcacgg cttcgatgtg ccgctgtccg atggcgccgc gttcgaccgg ttgcgcacga
     3061 gtctgcagcg cgtggccgac gagttgggca agacgctgat ccccgtacgc accaacatcc
     3121 gcgtcacgag gtttcgcgag ctcgagtggg cgcgcctcgg tcacggaccg gcgctcgcgg
     3181 ccatcgcgct ggccctcgag ccg
//
LOCUS       DASUCJ010000360         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_523492, whole genome shotgun sequence.
ACCESSION   DASUCJ010000360 DASUCJ010000000
VERSION     DASUCJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523492"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFU90_04345"
     CDS             complement(<1..533)
                     /locus_tag="VFU90_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479036.1"
                     /translation="MSDAVSHGFPPRGNAWKSRDVLRAAAIVFGLYFTLRLLWFANAL
                     VLVAFLGVLFGLAISAGVDQLARVRVPRAIGAPTIVLAFFALLYGAGALIAPTVSNQL
                     AVLKSRLPEAQARVEGWLAQRGGLMRAAMGSGPAADTGRAAAPQGQLGGPLAPVGRGA
                     AEATQRDTVGSGTSNPLA"
     gene            699..1880
                     /locus_tag="VFU90_04350"
     CDS             699..1880
                     /locus_tag="VFU90_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU5479037.1"
                     /translation="MPSHSSPPSHPRPATRGSGTSTRYIDTPADAERFLASIGEPNRL
                     AVDTEGASFHRFVDRIYLLQISTESVNAILDPLMIGKPRALGELLQSTRSETVFHDAD
                     YDLRLLFQDYGWHARRLFDTRIAAQLLGIRAFGLAALLEQFLGIRLDKKHQRADWSMR
                     PLPQDMLDYATQDTMYLLPLRDRLAEALEGAGRTAWAREEFDRLEGTRWEHDASADAY
                     QRIKGARDLSRRELAVLRELVAWRDGVARELDRATFRVAGNETLLELSRATPRTVDAM
                     REIRGMPRGVIERRGADVLDAIARGVAVPDEDLPRFPRAARWERDPDFDARVNRLRSI
                     RETAAQRLDLDPGFLISRERLETIARKRPTSLEELAQADDLRQWQIDVMGKDLLRALD
                     G"
     gene            complement(1907..2260)
                     /locus_tag="VFU90_04355"
     CDS             complement(1907..2260)
                     /locus_tag="VFU90_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5479038.1"
                     /translation="MNDDTPQPKPPDAPGTPATALTEDVVKATLRRVKDPELNLNIVD
                     LGLVYAVTVDGGLVTVDVTLTSPGCPSGGEILTTAEQQIRSLPGVEDVVMNLVWDPMW
                     SPDRMEPRIKAYLGF"
     gene            complement(2321..3673)
                     /locus_tag="VFU90_04360"
     CDS             complement(2321..3673)
                     /locus_tag="VFU90_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5479039.1"
                     /translation="MVLALLAAAAIAAGNGGLPVKAPASVGMSAARLRVIDRVVLRGI
                     HAGGFPGAAVVVGRKGAVVWEKGFGRIDWKSSSPRVSPSETLYDLASLTKVVGTTTAI
                     MILYDKGLFKLDDPVVKYLPEFGGGEKDSVTIRELLEHRSGLPADRELWRLASTPEEA
                     RHIVLSTPLDYKPGAQYVYSDLGAMTLGFLVEKLSGQPLDEFLEMWVFHPLGMMNTFY
                     RPADSVKYRAAPTEDTPPRGYPLKGEVHDENAYALGGVAGHAGLFSTAADLSIFAQMI
                     LNGGEFNGTRIVADSTIRLFTHRAAGSRALGWAMADGQWGSGRYLSDEAFGHVGYTGT
                     SLWIDPDRDMFVILLTNRVHEPRARRPEKVIADVRADLSDAAAVAVTDDPDQLIAMPV
                     SFRADKAKGWNHVEHRHHRRVRRRGTKSTVRITKHHTVTSSHSKKAVATPKKNHTTKK
                     "
     gene            4069..>4618
                     /locus_tag="VFU90_04365"
     CDS             4069..>4618
                     /locus_tag="VFU90_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5479040.1"
                     /translation="MASARSATEVDATVTNQANGMETDHALIRRAVGGDERALRQLWS
                     QYSPHIDAVVRRLVGGDPDLAADIAQDVWIQIFRALGSYRGDAQFGTWAHRIAVNRTL
                     NALRKARRLGRLEVDIEEDSSAVEMDSDRAVLAASIEDATARLPAGARTVFLLHDVEG
                     YTHDDIARELGITTGASKSQLFK"
BASE COUNT          805 a         1557 c         1512 g          744 t
ORIGIN      
        1 gctaacggat tcgacgtgcc cgagcccacc gtgtcgcgct gcgtcgcctc ggccgccccg
       61 cgcccgacgg gcgcgagcgg accgcctaac tgaccctgcg gcgccgccgc gcggcccgta
      121 tctgccgccg ggccggagcc catcgctgcc cgcatcaacc cgccgcgctg cgcgagccat
      181 ccctcgacgc gcgcctgcgc ctctggcaaa cgcgacttga gcacggcgag ctgattgctc
      241 accgtcggcg cgatcagcgc gcccgcgcca tagagaagcg cgaaaaaggc cagcacgatc
      301 gtcggtgcgc cgatggcccg tggcacgcgc acccgcgcca attgatccac accggccgag
      361 atcgccaggc cgaacaacac gcccagaaac gccaccagca ccagcgcgtt cgcgaaccac
      421 aacaaccgca gcgtgaagta caaaccgaac acgatggccg cggcgcgcaa cacatcgcgc
      481 gacttccagg cattcccgcg cggcgggaac ccgtgtgata ccgcgtcgga catggacacc
      541 gtcgcagaga gagtgtcgat accgctacga gtggactcca aagtagcagt ccgcttcctt
      601 tcgcgcgtcc cggacgagag aattcacgaa caccccggtg cgaccgcgcc agcccgggga
      661 ccgccaccgc ttggcctgct acacgaggta cgctccgagt gccgtcgcat tcttcaccac
      721 cgtcccaccc tcgaccggcc acgcgcggct ccgggacttc cacgcggtac atcgataccc
      781 cggctgacgc cgagcgcttt ctcgcctcga tcggcgaacc caaccgattg gccgtcgaca
      841 ccgagggcgc cagcttccac cggttcgtcg accgaatcta cctcctccaa atcagcacgg
      901 aatccgtgaa cgccattctc gatccactta tgatcgggaa accgcgcgca ctcggcgagc
      961 tgctgcagag cacgcggtcg gaaaccgtct ttcacgacgc cgactacgac cttcgcctct
     1021 tgtttcagga ctacggctgg cacgcccgcc gtctgttcga cacgcgcatt gccgcacagc
     1081 tgctcggaat ccgcgcgttc ggactcgccg cgctgctcga gcaattcctc ggtatcaggc
     1141 tcgacaagaa acaccagcgc gccgattggt ccatgcggcc gctgccgcag gacatgctcg
     1201 actacgccac ccaggatacg atgtatctgc tgccgctccg cgaccgcctc gccgaagcgc
     1261 tggagggcgc cggacgaacc gcgtgggcgc gcgaggagtt cgatcgactc gagggcacca
     1321 ggtgggagca cgatgcttcg gccgacgcct atcagcggat caagggtgcg cgcgacttga
     1381 gtcgtcgcga gctcgcggtc ctgcgagagc tggttgcctg gcgcgatggc gtggcccgcg
     1441 agctggatcg cgcgacgttt cgcgtggccg gaaacgagac gctgctggag ttgtctcgcg
     1501 ccacgccgcg cacggtggat gcgatgcgcg aaattcgcgg catgccgcgc ggcgtgatcg
     1561 agcgtcgcgg cgccgacgtg ctcgacgcga tcgcgcgcgg agtcgccgtt cccgacgagg
     1621 acctgccgag atttccgcgg gcggcgcgct gggagcgcga cccggatttc gacgcgcgcg
     1681 tcaaccgctt gcggagcatc cgcgagaccg ccgcccaacg gctcgatctc gatcccggct
     1741 tcctgatttc acgagagcgt ctggagacga tcgcccgcaa gcgtcccacg tcgctcgagg
     1801 agctggcgca ggcggacgac ctccgccagt ggcagatcga cgtgatgggg aaggacctgc
     1861 tgcgcgcgct cgacggctga tgcctaaccg ttaggcgccg acgcggtcag aagcccaggt
     1921 aggctttgat ccgcggctcc atccgatccg gactccacat cggatcccaa accaggttca
     1981 tcaccacatc ctcgacaccc ggcagcgatc gaatctgctg ttcggccgtg gtcaggatct
     2041 cgccacccga gggacatccc ggcgaggtga gcgtcacgtc caccgtcacg agtccgccgt
     2101 ccaccgtcac ggcgtacacc aaacccagat cgacgatgtt caggttgagc tcggggtctt
     2161 tcacccgccg cagcgtcgct ttgacgacgt cttcggtgag cgccgtggcg ggcgtgcccg
     2221 gtgcgtcggg cggtttcggt tgtggtgtgt cgtcgttcat gtcggctccg ggagactgca
     2281 taccgcaact tcaccggcgc cgagacggcg ggcaagactg ctacttcttt gtggtgtgat
     2341 tctttttggg cgtcgcgacc gctttcttgc tgtgcgacga cgtcaccgtg tgatgcttgg
     2401 tgattcgtac ggtcgacttg gtaccgcggc gccgcacgcg gcgatgatgg cgatgctcca
     2461 cgtggttcca ccctttcgcc ttgtccgcgc ggaacgagac gggcatcgca atcaactgat
     2521 ccgggtcgtc ggtcaccgca acggcggcgg cgtccgagag atcggcgcga acgtcggcga
     2581 tcactttttc aggccgccgc gcgcgcggct cgtgcacacg attcgtgagc aggatgacga
     2641 acatgtcgcg atcgggatcg atccagagcg acgtgcccgt atagccgacg tggccgaacg
     2701 cttcatcgct caggtagcgg cccgagcccc actggccgtc ggccatcgcc caaccgagcg
     2761 cgcggctgcc cgccgcgcgg tgcgtgaaga gccggatcgt cgagtcggcc acgatgcgcg
     2821 tgccgttgaa ctcgccgccg ttcaggatca tctgcgcgaa aatcgacagg tccgccgcgg
     2881 tgctgaagag cccggcgtgt ccggccacgc cgcctaacgc ataggcgttt tcgtcgtgga
     2941 cctcgccctt gagcgggtag ccgcggggcg gcgtgtcctc ggtgggcgca gcccgatact
     3001 tgacggagtc ggccgggcgg tagaaggtgt tcatcatgcc taacggatgg aacacccaca
     3061 tctcgaggaa ctcgtccagc ggctggccgc tcagcttctc gaccaggaac ccgagcgtca
     3121 tcgcgcccag gtcggagtac acgtattgtg cgccgggctt gtagtcgagc ggcgtgctga
     3181 gcacgatgtg gcgggcttct tcgggcgtcg aggcgaggcg ccacagctcg cgatccgccg
     3241 ggaggcccga ccgatgctcg aggagctctc gaatcgtcac gctgtccttc tcgccgccgc
     3301 caaactcggg cagatacttg acgaccgggt catcgagctt gaagagaccc ttgtcgtaca
     3361 aaatcatgat ggcggtggtc gtgccgacga ctttggtgag cgaggcgaga tcgtacaacg
     3421 tttcggaggg cgagacgcgg gggctggacg acttccaatc gatgcggccg aagcctttct
     3481 cccaaacgac cgcgcccttg cgacccacta cgacggccgc gccggggaac ccgccggcgt
     3541 gaatgccgcg cagcaccacc cggtcgatca cgcggagccg cgcagccgac atgccaacgg
     3601 acgccggtgc tttgaccggc agacctccat tcccagcagc gatggcagcg gcagcgagca
     3661 gcgcaagaac cacgatgaat ccctcttccg aagcgactgc gggaacctcg actcgcggag
     3721 gcgtgtccaa atgccacgac tcgaagcttg gggttgtacc ccgcgctcac gatacggcgg
     3781 cggacttgaa ttgtcgcaag aacagagcag cagcagagcg aaccgcgaac acgaaccgga
     3841 atctggcggg ctgacgtccg gcagaacgac cacacgactt gaggtcgggt gagctatcct
     3901 agtccaatat gtggggttgc attcatttgg caagacgaaa gacatcggtg ggcgtcacct
     3961 tttgcacgcc ggcggactcg agtgtaatgg tggtgcgtcc gcttcggctt gccgtggggg
     4021 cgcgtcatcg ctcggtcgct tcgaccggag gtttcgtgga ggccggccgt ggcgagcgct
     4081 cgttcggcaa ccgaagttga cgcaacggtg accaatcaag caaacggaat ggaaacggat
     4141 cacgctctca ttcggcgcgc cgtcggcggc gatgagcggg cgctgcggca gctgtggtcg
     4201 cagtactcgc cgcacatcga cgcggtcgtg cggcggctcg tgggcggcga tccggacctc
     4261 gcggcagaca tcgcgcagga cgtgtggatc cagattttcc gcgcgttagg cagctaccgc
     4321 ggcgacgcgc agttcgggac gtgggcgcac cgcatcgcgg tgaaccggac gctgaacgcg
     4381 ctgcgcaagg cgcggcggct ggggcggctg gaggtcgaca tcgaggaaga ttcctcggcc
     4441 gtcgagatgg acagcgaccg ggcggtcctc gccgcctcga tcgaagacgc gacggcgcgg
     4501 ctccccgccg gggcgcgcac ggtgttcctc ctgcacgacg tggaaggcta tacgcacgac
     4561 gacatcgccc gtgagttagg catcacgacg ggcgcatcga aatcgcaact cttcaagg
//
LOCUS       DASUCJ010000361         1471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1149953, whole genome shotgun sequence.
ACCESSION   DASUCJ010000361 DASUCJ010000000
VERSION     DASUCJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149953"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..370
                     /locus_tag="VFU90_04370"
     CDS             164..370
                     /locus_tag="VFU90_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479041.1"
                     /translation="MTLLFTDVEESTRLLQELGDGYGDALPARPARTRLEAELPPQQL
                     AAAREAAAAATLEDHERTARRELV"
     gene            complement(496..1206)
                     /locus_tag="VFU90_04375"
     CDS             complement(496..1206)
                     /locus_tag="VFU90_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479042.1"
                     /translation="MLTGARVLLTGATGRFGRVLASELIRNDCEVTLIVRAPSPETAR
                     ARVASVLGTDFRQVKLVCGDVTEPFFGASARERAWLRLSTDVVVHAAAQTSFGSSLEI
                     ARRANVHATRNTLEFAERMPRLLRFAYVSTAFVAGKRMGRILETELEHSHGFHNAYQQ
                     SKYEAEFLVRGRDDVLPVVVLRPAIVLERSDAVTQRRCLTRRRRCCHSTLGIKAATPS
                     SRRALDDETSAENRDRHP"
     gene            complement(1200..>1471)
                     /locus_tag="VFU90_04380"
     CDS             complement(1200..>1471)
                     /locus_tag="VFU90_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479043.1"
                     /translation="ELPNSFVKRRLGIAPGSLSRRHRVGQYIADQADSPAGGTLALAF
                     FLGLEADTLLIVFAVGFCLHVVMDQLYVLFAVKRRSSPPAVSPTC"
BASE COUNT          250 a          487 c          498 g          236 t
ORIGIN      
        1 tcgaacaagc cgcttgagca gcagacagca agaggctcga aaccgttacc cgtcgttagg
       61 cggatcgagc gttcaaatcc ctccccctcc gcttcttgtt cgctggacag cgaacgcgcg
      121 acggcgcatg atcgtgtgcg atggcgagcg gtctgccgac gtcgtgacgt tgctgttcac
      181 cgatgtcgag gagtccacgc ggctcttgca ggaactcggc gacggctacg gggacgctct
      241 ccccgcgcgc ccggctcgca cgcggctcga ggccgaactg ccgccccagc agcttgcggc
      301 cgcccgcgaa gcggccgccg cggcgacgct tgaagatcac gagcgaacgg cccgccgcga
      361 actcgtctga cgctttctcc ctggtgggag tggcccggcg cgaactccgg cgcgcgcctg
      421 acgacggata acggttttcg agcctcttcc tcccgcctga ttcagccgaa aactgcggag
      481 gcctgatccg cggatctacg gatgtcggtc gcggttttcc gcggaagtct cgtcatcaag
      541 agccctgcgg ctggatggtg ttgcggcttt gattccgagg gtggaatgac aacagcggcg
      601 tcgcctggtg aggcagcgtc gctgcgtgac tgcgtccgac cgttccagca cgatcgcggg
      661 ccgcagaacg accacgggca gcacgtcatc gcgcccgcga acgaggaact cggcctcgta
      721 cttcgactgc tggtacgcat tgtggaagcc gtggctgtgc tcgagctctg tctcgaggat
      781 ccgccccatg cgtttgccgg cgacgaacgc ggtgctgacg taggcgaacc ggagcaaccg
      841 cggcatgcgt tctgcgaact cgagcgtgtt gcgcgtcgca tggacgttcg cgcgccgggc
      901 gatttcgagc gacgagccga agctcgtctg cgccgccgcg tgcacgacga catccgtcga
      961 gaggcggagc cacgcgcgct cacgcgccga cgctccgaag aacggctccg tcacgtcccc
     1021 gcagacgagc ttcacttgcc ggaagtcggt gccgagcaca ctcgctaccc gcgcacgtgc
     1081 ggtttccggc gagggcgcgc gcacgatgag cgtgacctcg caatcgttgc ggatcagctc
     1141 actggcgagg acgcgtccaa atcgtccggt cgctcccgtc agcagtacgc gcgcgccggt
     1201 cagcatgtcg gggacaccgc cggaggcgag cttcgccgtt tgacagcgaa gaggacgtag
     1261 agctgatcca tcacgacgtg caggcagaag cccacggcga acacgatcaa aagcgtgtcg
     1321 gcctcgaggc cgaggaagaa cgccagcgca agcgttccac ccgcgggcga gtccgcctgg
     1381 tcggcgatgt actggcccac gcggtgtcgc cgcgagaggg aaccgggggc gatcccgagg
     1441 cggcgcttga cgaaactgtt cggcagttcg g
//
LOCUS       DASUCJ010000362         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1073944, whole genome shotgun sequence.
ACCESSION   DASUCJ010000362 DASUCJ010000000
VERSION     DASUCJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073944"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1018
                     /locus_tag="VFU90_04385"
     CDS             <1..>1018
                     /locus_tag="VFU90_04385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU5479044.1"
                     /translation="RKLRDMGLGAEVLIADGVRGDAAAEALASKLAADPAVEFAEVDH
                     LRHPLGSFPVNDQYIGSQFYLFTGSTGINAFDAWAVTHGSPNVTVAVIDTGYRPHIDL
                     AGRFLPGYDMISDPAVANDGGGRDADASDPGDWLTTLDVQSGTFAGCEARWSTWHGTS
                     VSGVIAANVNNIAYTAGIDWNAKILPVRVLGRCGGYDSDIVDGMLWAAGLSVPGVPDN
                     PHPAQVLNLSLGGESACPSVYPSAIAAIYRHGITRAIVVAAGNSAEDVSKSSPANCPG
                     VISVASTTTTGNLAAYSNFGPGILVSAPGGSANPSFTDQVVVVLSNLGSEGPTTDTVK
                     YEGGT"
BASE COUNT          165 a          342 c          340 g          171 t
ORIGIN      
        1 tgcgcaagct tcgcgacatg gggctcggtg ccgaggtgct gatcgccgat ggtgtccgag
       61 gcgatgcggc agccgaagcg ctcgcgtcga agctcgccgc cgatccggca gtcgagttcg
      121 ccgaggtcga tcacctgcgc catccgctcg gatcgtttcc ggtcaacgac cagtacatcg
      181 gatcgcagtt ctatctcttc accgggtcga ccggcatcaa tgcgttcgac gcgtgggcgg
      241 tgacgcacgg gtcgccgaac gtgacggtcg cggtgatcga caccggctac cggccgcaca
      301 tcgatctcgc cggacgtttc cttcccggct acgacatgat ctcggatccg gccgtggcga
      361 acgacggcgg cggccgcgat gctgatgcca gcgaccccgg cgactggctg acgacactcg
      421 atgtgcaaag cgggaccttc gccggctgcg aggcgcgctg gagcacctgg cacggcacct
      481 cggtctccgg agtcatcgcg gccaacgtga acaacatcgc ctacacggcg ggcatcgact
      541 ggaacgcgaa gatcctgccg gtgcgcgtgc tcggccgctg tggtggttac gattccgaca
      601 tcgtcgacgg catgttgtgg gcagccggcc tgtcggtgcc cggcgtcccg gacaatccgc
      661 atcccgcgca ggtcctgaac ctgagcctcg gcggcgagag tgcgtgcccg tccgtctatc
      721 cgtcagcgat cgcggcgatc taccggcacg gcatcacgcg tgcgatcgtc gttgcagcgg
      781 gcaacagcgc cgaggacgta tcgaagtcct cgccggcgaa ttgccccgga gtgatttccg
      841 tcgcgtcgac gacgacgacc ggcaatctcg cggcgtacag caacttcggc cccggcatcc
      901 tcgtatcggc gcccgggggc agcgccaatc cgtcgttcac cgatcaggtc gtcgtcgtgc
      961 tgtcgaatct cggcagcgag ggcccgacga cggatacggt caagtacgaa ggcggcac
//
LOCUS       DASUCJ010000363         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_400985, whole genome shotgun sequence.
ACCESSION   DASUCJ010000363 DASUCJ010000000
VERSION     DASUCJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400985"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..626)
                     /locus_tag="VFU90_04390"
     CDS             complement(63..626)
                     /locus_tag="VFU90_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU5479045.1"
                     /translation="MQGVEGKRIAALIGDGFEEIDLLEPRRALEDAGAVVTIVGIDER
                     ARQRIRGKRGLDDGQSVRAEELVADCTAEDFDALLVPGGLSPDRIRADREVQRFCREF
                     DVAKKPMFSIGHGAHVLISSQLARGRQITGAHSIADDIRNAGGLYRDQPTVTDSNWVS
                     TRGGDDMPQFNRAMLEKLAAAAPPQPV"
     gene            complement(651..1154)
                     /locus_tag="VFU90_04395"
     CDS             complement(651..1154)
                     /locus_tag="VFU90_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479046.1"
                     /translation="MTERATWDGREGTAGGAAFGAALESLARENEVTPETLAEAAGLS
                     AGEFAEVVAGSRRVGISALARFAVTLRLRPIEFLQRAHILSLEVYAFGLDPLYFLPEG
                     PVRYDARIYMREINPRHRVPEGDMTRRNPTLKALAGDGLLDALGKTEVELAYLLRAAV
                     EQTGGTL"
     gene            complement(1151..>1341)
                     /locus_tag="VFU90_04400"
     CDS             complement(1151..>1341)
                     /locus_tag="VFU90_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5479047.1"
                     /translation="PHQGGPVGEGIVEGATVTCPWHAWCFDLRTGTLTLGDFARLRSF
                     EAYVEAGSIFVSTEPSSE"
BASE COUNT          225 a          460 c          438 g          218 t
ORIGIN      
        1 ctgaaagaaa tccgcgccgc tatcgtttac gcgacgggct gtaaagccgc gggccgagga
       61 tcctagacgg gttggggcgg ggcggcggcg gccagcttct cgagcatcgc gcgattgaac
      121 tgcggcatgt cgtcgccgcc gcgggtcgag acccagttcg aatcggtaac ggtcggctgg
      181 tcgcgataca gtccgccggc attgcgaatg tcgtcggcta tcgagtgcgc cccggtaatc
      241 tggcgtccgc gcgcgagctg cgaggagatg agcacgtgag cgccgtgacc gatcgaaaac
      301 atcggcttct tggcgacgtc gaactcacgg cagaaccgct gcacttcccg gtcggcgcga
      361 atgcggtcgg gcgaaaggcc cccggggacg agcaaagcgt cgaaatcctc ggcggtgcag
      421 tcggcgacca gttcctcggc ccgcacgctc tgcccgtcgt cgagaccccg tttgccccga
      481 attcgctggc gggcgcgctc gtcgatgccg acgatcgtca cgaccgcacc ggcatcctcg
      541 agggcgcggc gcggctcgag caaatcaatt tcttcgaatc cgtcgccgat cagggccgcg
      601 attcgctttc cttccactcc ttgcatcggc cccttcgttc gcggtgctcc ctagagggtc
      661 ccacccgtct gctcgaccgc ggctcgcagc aggtatgcga gctcgacctc cgtctttccg
      721 agggcatcca gcagaccgtc gccggcaagg gccttgagcg tcgggttgcg ccgcgtcatg
      781 tcgccctcgg gaacgcggtg gcgcggattg atttcccgca tgtagatgcg cgcgtcgtag
      841 cgcaccgggc cttcgggcaa aaagtagagc ggatcgagcc cgaaggcgta gacttccagc
      901 gacaggatgt gggcgcgctg caagaactcg atcggccgaa ggcggagcgt tacggcaaag
      961 cgagcgagcg cgctgatccc tacccggcgc gagcctgcaa ccacttcggc aaactcgcca
     1021 gccgacagcc cggccgcctc cgcaagcgtt tccggcgtca cctcgttctc gcgagccagg
     1081 ctctccaaag cggcaccgaa ggcggcgccg cccgccgttc cttcgcggcc gtcccacgtc
     1141 gcgcgctcgg tcactcggag ctcggctcgg tggaaacgaa aatcgatccg gcctccacgt
     1201 aggcttcgaa agaacgcagc cgtgcgaaat cgccgagcgt cagcgtcccg gtgcgcagat
     1261 cgaagcacca ggcgtgccac ggacaggtca ccgtcgcgcc ctcgacgatt ccttcgccga
     1321 ccggaccgcc ttgatgcgga c
//
LOCUS       DASUCJ010000364         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_462564, whole genome shotgun sequence.
ACCESSION   DASUCJ010000364 DASUCJ010000000
VERSION     DASUCJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462564"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="hslU"
                     /locus_tag="VFU90_04405"
     CDS             <1..1264
                     /gene="hslU"
                     /locus_tag="VFU90_04405"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU5479048.1"
                     /translation="KNILMIGPTGVGKTEIARRLASLVGAPFVKVEATKYTEVGYVGR
                     DVESMVRDLVEASVRTVTDERREEVRDLAERHAVERLIDVLHPETRPPSAQQAGNPFA
                     ATLGSIFGSNYAPSQPTQPSPSPGASAETQRVREQTRSEIERGFYDVRLIEIEVEETP
                     NIPIGMIGAPEGGGADIGEMLGGILPKRRTKKRVTVAEARRIFEQEEAQKLIDMDAVK
                     REALRRAAEDGIIFIDEIDKVAAQGSRGGPDVSREGVQRDILPIVEGSTVNTKYGSVK
                     TDHVLFIAAGAFHISKPSDLIPELQGRFPIRVELDSLTADDFKTILTQPKNALVEQYK
                     ALLATENATLDFRPDGIEQIATFAMRVNETSENIGARRLHTVLERLLEDISFAAPEET
                     GTYVIDAAYVRQRLADIVEDADLSGYIL"
BASE COUNT          284 a          436 c          454 g          217 t
ORIGIN      
        1 gaagaacatc ttgatgatcg gcccgacggg ggtcgggaaa accgagatcg cgcgccggct
       61 ggcaagtttg gtgggcgcgc cgttcgtaaa ggtggaggcg acgaagtaca ccgaggtcgg
      121 ctacgtcggg cgcgacgtcg aatcgatggt gcgcgacttg gtcgaagctt cggtacgcac
      181 cgtcaccgac gagcgccgcg aggaagtgcg cgacctcgcc gagcgccacg ccgtcgagcg
      241 gctcatcgac gtgctgcatc cggaaacgcg cccgccctcc gcgcagcagg ccggcaatcc
      301 gtttgcggcg acgctcggat cgatcttcgg cagcaactac gcgccctcgc agcccacgca
      361 gccgtcgccc tcacccggcg cctcggccga gacgcagcgc gtccgcgaac agacgcgttc
      421 ggagatcgag cggggcttct acgacgtccg gctgatcgaa atcgaggtcg aagagacgcc
      481 caacattccg atcgggatga tcggtgcgcc ggaaggcggc ggagccgata tcggcgagat
      541 gctcggcgga attctgccga aacgccgcac caaaaagcgc gtgaccgttg ccgaagcgcg
      601 ccgcatcttc gagcaagagg aagcgcaaaa gctgatcgac atggacgcgg tcaagcgcga
      661 agcgctgcgg cgggcggccg aagacgggat tatcttcatc gacgagatcg acaaagtcgc
      721 cgcccaaggt tcgcgcggcg gacccgacgt ttcgcgcgag ggcgtgcagc gcgacatttt
      781 gccgatcgtc gaagggtcga ccgtcaacac gaagtacggc tcggtcaaga ccgaccacgt
      841 gctgtttatc gcggcgggcg cctttcacat cagcaaaccc tcggacttaa ttccggagct
      901 gcaagggcgt ttcccgatcc gagtcgagct ggattcgctg accgccgacg atttcaagac
      961 gatcctcacg cagccgaaga acgcgctggt ggaacagtac aaggcgctgc tcgcgacgga
     1021 gaatgcgacc ctcgactttc ggccggacgg aatcgagcaa atcgccacct tcgcgatgcg
     1081 cgtgaacgag acgagcgaga acatcggcgc tcgccggctg cacaccgtgc tggaacgctt
     1141 gctggaagac attagcttcg ccgccccgga ggagacgggc acctacgtga tcgatgcggc
     1201 ctacgtccgc cagcggctcg ccgacatcgt ggaggatgcc gacctctcgg gttacattct
     1261 ctagcaggcc gcgggttttc aggaacctcc gaataacctt ggtctatact gggaaggctc
     1321 tgggaacttc ctaatggttt tcccatcccg ccggaaggac gggaagccgt cgcgagagga
     1381 ttcgccaacc a
//
LOCUS       DASUCJ010000365         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_645920, whole genome shotgun sequence.
ACCESSION   DASUCJ010000365 DASUCJ010000000
VERSION     DASUCJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645920"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="ligA"
                     /locus_tag="VFU90_04410"
     CDS             complement(<1..1173)
                     /gene="ligA"
                     /locus_tag="VFU90_04410"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5479049.1"
                     /translation="MTSPNDRPSDCRRLEDRARELRGLIERANHCYYVLDAPEISDRE
                     YDLLMRELQQVEEQCPELRTPDSPTQRVGAPIQGGLAKVIHMVPMLSLGNAFDDDELR
                     DWETRAVRIAGADVRTAGYTAELKIDGNAVNLTYERGLLVRGATRGDGTEGEDVTVNL
                     RTIHDIPLRLDTPNPPDLIEVRGECYMTFDGFEKLNDRRREQDEKVFANPRNSAAGSL
                     RQLDPSITAQRRLRFFGYAAAPAPGVRLPFTTQWGLLDTLESWGFPVEPHRRRCKTLD
                     EVVSFVQEVEHALRAKLAFGIDGVVVKVDALNVQDELGDVGREPRWAVARKFAPDIEV
                     TRLLDIKVNVGRTGALNPYAELEPVEISGVIVKLATLHNEALVRQKDLRIGDWVQVK"
BASE COUNT          214 a          446 c          434 g          246 t
ORIGIN      
        1 cttcacctgc acccaatcgc cgatgcgcag gtctttctgg cggaccaacg cttcgttgtg
       61 cagcgtggcg agcttcacga tcacgccgct gatctcgacg ggttcgagct ccgcgtacgg
      121 attgagcgca ccggtgcggc cgacgttcac tttgatgtcg agcagcctgg tgacttcgat
      181 gtccggcgcg aacttccggg ccacggccca ccgcggctcg cggcccacgt cgcctaactc
      241 gtcctgcacg ttcagcgcat cgaccttgac gacgacgcca tcgatgccga acgccagctt
      301 ggcgcgcagc gcgtgctcga cctcttgtac gaacgacacg acttcatcga gcgtcttgca
      361 ccggcggcgg tgcggctcga cggggaagcc ccagctctcg agcgtatcga ggagtcccca
      421 ctgcgtggtg aacggaaggc ggaccccggg cgccggcgcc gcggcatatc cgaaaaaccg
      481 cagccggcgt tgcgccgtga tcgacggatc cagctgccgg agcgaccccg ccgccgagtt
      541 gcgcggattg gcgaaaacct tttcgtcttg ctcacggcgg cggtcgttga gcttctcgaa
      601 gccatcgaaa gtcatgtagc actcgccgcg cacttcgatg aggtcgggcg ggttaggcgt
      661 gtcgagccgg agcgggatgt cgtggatggt gcggagattc accgtgacgt cttcgccttc
      721 cgtcccgtcc ccgcgggtcg caccgcgcac caacaggccg cgctcgtacg tgagattgac
      781 cgcgttgccg tcgatcttga gctccgcggt gtagccggcc gtgcgaacat cggcgccggc
      841 gatgcgcacc gcgcgcgtct cccagtcgcg gagctcgtcg tcgtcgaacg cattgccgag
      901 agagagcatg ggcaccatgt gaatgacctt ggcgagcccg ccttggatgg gcgcgcccac
      961 gcgctgcgtc ggcgagtcgg gtgtccggag ctcgggacac tgctcttcga cctgctgtaa
     1021 ttcgcgcatc aggaggtcgt attcgcgatc cgagatctcg ggcgcgtcga gcacgtagta
     1081 acagtggttc gcgcgctcga tgagaccgcg gagttcgcgg gcccgatctt cgagacggcg
     1141 gcagtcggag ggacgatcgt tcggagacgt cacgaacgaa aaatatgtga atggggaagc
     1201 gcgcgcctcc ctgcctagct cttcgcctct tcccccacga cttcgagggt caccgacacc
     1261 agccgctcca gctcctcgtc cgccgacgtc gccaacagcg tctccgccca catgtgatct
     1321 tcggtattcg tgtagcggtc
//
LOCUS       DASUCJ010000366         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_126325, whole genome shotgun sequence.
ACCESSION   DASUCJ010000366 DASUCJ010000000
VERSION     DASUCJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126325"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFU90_04415"
     CDS             <1..409
                     /locus_tag="VFU90_04415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5479050.1"
                     /translation="MGTEDEAAAVIAHEIEHIDNGQVSRRIDSLVTSMHRDVTNASEW
                     RWDEFGASYGQVLEYRCDSLGAELVVKAGYSPYAYKTMMETFIALGKVHAPNATPARE
                     FTDRVAQIERQIAEMHWDALTKTRPLRLPGTGG"
     gene            complement(466..1947)
                     /locus_tag="VFU90_04420"
     CDS             complement(466..1947)
                     /locus_tag="VFU90_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5479051.1"
                     /translation="MRWWRDITVIAADSMHGRRTGTADYVEAARYVASQFQAAGLAPG
                     GSDGYFQTVHLAEARLVPESSSVVLESSGRSDTLPLRVRITPSSTGRADGSLVFVGYG
                     LALPGVHDDLAGVDVHGKVAVYLDRMPKGLNATLFAHGRASRWKELERRGAVAGIAIA
                     DPAPPNGNRPALRPGARPVMGLADDSLERGVLVSVRGEDAEQLFAGSGHTYAEMVALS
                     DAQKTLPTVPLAPRLHVRLDIQRTPVDAPNVVGVLRGSDAKLRDQYLVVSAHLDHLGV
                     GRPVNGDSIYNGAMDNASGIATLLETARAFKERHVRPKRSVIFLAVTGEEEGELGSAY
                     FALHPTVPASGIVADLNTDMYLPIQPLTGVFAYGWNESDLGKDILPVLRQHHLANLED
                     PEPEQVRFIRSDQYSFIQRGIPALAFKVGYTKDSPGDSTVTQWLDTRYHKPSDDLAQP
                     VNFETAIAFDAIYFDIVRAVADRPTRPAWYPTSVFASIKRPGQ"
     gene            2234..>2848
                     /locus_tag="VFU90_04425"
     CDS             2234..>2848
                     /locus_tag="VFU90_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5479052.1"
                     /translation="MMRAHLTSVSVGAACITAAAWAAVAFTPGAIAGPRITPAAASDD
                     GEWTMPGKDYASTRYSGLSQITPANAAHLHPVWSFSTGVLGGHEGQPLVVHNTMYVVT
                     PYPNALYAFDLTQPDYPLQWKYRPDVSPNAVGVACCDAVNRGAFYADGKIVYNLLDGH
                     TVAIDAQSGRQLWETTIADVSNGETTPMAPLVVKNRVLVGASGGE"
BASE COUNT          505 a          944 c          943 g          456 t
ORIGIN      
        1 catgggcacg gaggacgaag cggcagcggt gatcgcccac gagatcgagc acatcgacaa
       61 cggccaggtt tcacgccgga tcgacagcct ggtgaccagc atgcaccgcg acgtgaccaa
      121 cgcgtctgag tggcgctggg acgagttcgg cgcgagctac ggccaggtgc tcgagtaccg
      181 ctgcgattcg ttaggcgcgg agctcgtcgt caaggcgggc tactcgccct acgcctacaa
      241 aacgatgatg gagaccttca tcgcgctggg caaggtgcat gcgcctaacg caacgccggc
      301 cagggagttc accgaccgcg tcgcgcagat cgagcggcag attgccgaaa tgcactggga
      361 cgccctcacg aagacgcggc cgctgcggct gcccgggacc ggtggatgat cgcgaaacgt
      421 ctcgatcgtg cgcgatggca gtagatcgcc gcccgcggtg tcgccctact gccccgggcg
      481 cttgatcgag gcgaacacgc tcgtcgggta ccacgccggc cgcgttgggc ggtcggcaac
      541 ggcgcgcacg atgtcgaagt atatcgcgtc gaacgcgatg gcggtctcga aattcaccgg
      601 ctgggcaaga tcgtccgacg gcttgtggta gcgcgtgtcg agccactgcg tcaccgtcga
      661 atcgcctggc gaatctttcg tgtaaccgac cttgaacgcc agcgccggga tgccgcgttg
      721 aatgaagctg tactggtcgc tgcgaatgaa gcgaacctgc tccggctcgg gatcctcgag
      781 attcgccagg tgatgctgcc tgagcaccgg cagaatgtcc ttgcctaaat cggattcgtt
      841 ccagccgtac gcgaacacgc ccgtgagcgg ttgaatcggc agatacatgt cggtgttgag
      901 gtcggccacg atccccgacg ccggcacggt gggatgcagc gcgaagtacg cggagccgag
      961 ctcgccctcc tcttcgccgg tcacggcgag gaagatgacc gagcgcttcg gcctcacgtg
     1021 tcgttccttg aacgcgcgcg ccgtctcgag cagcgtggcg atgcccgacg cgttgtccat
     1081 cgccccattg tagatgctgt cgccgttcac cggccgccca acgcccaggt gatccaggtg
     1141 cgcgctcacg acgagatact ggtcgcgcag cttcgcgtcg ctgccgcgca gcacaccgac
     1201 gacgttcggc gcgtccaccg gcgtgcgctg gatgtccagg cgcacgtgca atcgcggtgc
     1261 gagcggaacc gtcgggagcg ttttctgcgc gtcggacagc gcgaccatct cggcatacgt
     1321 gtggcccgac ccggcgaaca gttgctcggc gtcttcgccg cggacgctca ccaacacgcc
     1381 gcgttcgagc gaatcgtcag ccagtcccat cacgggccgc gcccccggcc gcagagccgg
     1441 ccggtttccg ttaggcggag ccggatcggc gatcgcgatg ccggcaaccg cgccgcgccg
     1501 ctcgagctcc ttccaccgcg acgcgcgccc gtgcgcgaag agcgtcgcgt tgagtccctt
     1561 gggcatgcga tcgagataca cggccacctt gccgtgcacg tcgacgcccg cgagatcgtc
     1621 gtgcaccccc ggtaaggcga ggccgtagcc gacgaacacc agcgagccat ccgcccggcc
     1681 cgtcgacgat ggcgtaatac gcacccggag cggcagcgtg tcgctcctgc ccgacgactc
     1741 caacaccacg ctcgagctct cgggcacgag acgcgcctcg gccaggtgca cggtctggaa
     1801 gtatccatcg ctgccgcctg gtgcgaggcc cgccgcctgg aactggctcg cgacataacg
     1861 agcagcctcg acgtagtcgg cggtgccggt gcgccgcccg tgcatactgt cggcggcgat
     1921 gacggtgatg tcgcgccacc agcgcacggc gcctggaggc ggagtctgtt gggcgtgaag
     1981 cgacggaagc gcagaagcca gcgcccaagc ggcgacgagt acggcagaag agcgacgacg
     2041 acgtgtcata cgacgatggt tgtagagaga tgcgagtgat ttcaagctat tgaacgcacg
     2101 actcgggtgg atcgatgagc acggtttgcc ggtgagaagc aattctatga aatcgccctc
     2161 ggtcgcgcgc cgatagcatg gcgttcttcg ggcaacggag cgatgatcgt accgtcaccg
     2221 ctcgaggcga aacatgatga gggcccatct cacatccgtg tcggtcggag ccgcctgcat
     2281 cacggcagct gcgtgggcgg cggtcgcgtt cacaccggga gccatcgccg ggccgcgcat
     2341 cacgcctgca gcggcgtccg acgatggcga gtggaccatg ccgggcaagg actatgcgtc
     2401 cacccggtac agcggcctct cgcaaattac gccggctaac gccgcacatc tccatcccgt
     2461 gtggtcgttt tccaccggcg tgctgggcgg tcacgaaggg cagccgctcg tggtgcacaa
     2521 cacgatgtat gtggtgacgc cgtacccgaa cgcgctctac gcgttcgacc tcacgcagcc
     2581 cgactacccg ctccagtgga agtatcgccc cgacgtttcg cctaacgcag tcggcgtcgc
     2641 ctgctgcgac gcggtcaatc gcggcgcgtt ctacgccgac gggaaaatcg tgtacaacct
     2701 gctcgacggt catacggtcg ccatcgacgc ccagagcggc cggcagctct gggaaaccac
     2761 catcgccgac gtgtctaacg gagagacgac gccgatggcg ccgctcgtcg tgaaaaatcg
     2821 cgtgctcgtt ggcgcgtccg gcggcgaa
//
LOCUS       DASUCJ010000367         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1150008, whole genome shotgun sequence.
ACCESSION   DASUCJ010000367 DASUCJ010000000
VERSION     DASUCJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150008"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFU90_04430"
     CDS             complement(<1..502)
                     /locus_tag="VFU90_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479053.1"
                     /translation="MLVAASLLATLGSTVTAGHDLSDWFFSATAAQESGTASATIVPD
                     FLPGKSMSSMHCGGTGVAIGGGIWQLVKFDKTHDIGLAVASTDQCSVSVFKAAPPDAT
                     VPDADLSAMSTGRGLHVGSTYGDVLAAYGGKRITQSGRFVVTYASSVAGTGIAHPNKK
                     VENPETI"
     gene            complement(582..713)
                     /locus_tag="VFU90_04435"
     CDS             complement(582..713)
                     /locus_tag="VFU90_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479054.1"
                     /translation="MLIAHILGLPAEELLEPMTSGAFGAAAVMLAATVMSLARRLRH"
     gene            complement(723..1409)
                     /locus_tag="VFU90_04440"
     CDS             complement(723..1409)
                     /locus_tag="VFU90_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEU5479055.1"
                     /translation="MKTPPIVSPQEWEAARERLLVKEKQLTRARDALAAERRRMPWLA
                     VEKHYAFEGPNGKATLLDLFDGRRQLIVYRAFLEPGVIGWPEHACVGCSMIADQVADP
                     VHLNARDTTLAFASRAPQKDIQRVKERMGWQFPWYTITDGFDADFGVDEWHGTNVFFR
                     DGARVFRTYFVNNRGDEQLGGTWNYLDLTPLGRQEEWEDSPEGYPQSAPYEWWNWHDA
                     YGAGAPPLAK"
     gene            complement(1406..1885)
                     /locus_tag="VFU90_04445"
     CDS             complement(1406..1885)
                     /locus_tag="VFU90_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5479056.1"
                     /translation="MNDTRTPAAAPALVLRRVYAAPRQRVFDAWTKPELAIQFLGTGD
                     VTVPEARMDVRTGGSYTIVMQMPDGERWNVGGVYREVRPPERLSMTWRWEEDKPEDEY
                     DTLLTLEFNEVDGGTELVLTHEQFASADSRDRHQEGWTAIMAQADAVIAGPQARSSQ"
     gene            complement(1882..2226)
                     /locus_tag="VFU90_04450"
     CDS             complement(1882..2226)
                     /locus_tag="VFU90_04450"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5479057.1"
                     /translation="MVNNPERLDAVFSALGDPVRRQIVERLARGPQSVGEIASAFSIS
                     QPAISRHVRILEASGLLERQVLGRVHRCTLSPDAMKAASAWIDKQRTFWSAALDRLEH
                     LLAAPPTRKKKK"
BASE COUNT          366 a          739 c          758 g          452 t
ORIGIN      
        1 tgatggtttc gggattttcg accttcttgt tcgggtgagc gatgccggta ccggccacgc
       61 tggacgcgta cgtgacgacg aagcggccgc tttgggtgat tcgtttgccg ccgtaggccg
      121 ccagaacgtc gccgtacgtg ctgccgacgt gcaagccgcg cccggtgctc atcgccgaca
      181 aatcggcgtc gggtaccgtg gcgtccggcg gcgcagcctt gaagaccgag acgctgcatt
      241 gatcggtgct cgccaccgcc aatccgatgt cgtgcgtctt atcgaacttc acgagctgcc
      301 agataccgcc gccgatggcg acgccggtgc cgccgcagtg catcgaactc atcgacttcc
      361 cggggaggaa gtcgggtacg atcgtggcgg acgcggttcc cgactcctgc gccgcggtcg
      421 cgctgaagaa ccagtcgctc aagtcgtgac ccgccgtgac ggtcgatccc agcgtggcga
      481 gcagcgacgc ggcaacgagc aagtctcttg ctgaagaacg catgcagcgt tcaacttcgt
      541 gccctcgttt gccggctact gcagcggcga tccgccgtgc gttagtgcct caatcgacgc
      601 gcgagcgaca tcaccgtggc ggcgagcatc accgcagccg ccccgaacgc gccgctcgtc
      661 atgggctcca gcagctcctc ggccggcaag ccgaggatgt gtgcgatcaa catcgcgtcg
      721 gctcacttcg cgaggggtgg agctccggcg ccgtatgcgt cgtgccagtt ccaccattcg
      781 tacggcgcgc tttgcgggta gccctcagga gagtcttccc actcttcctg gcgtccgagc
      841 ggcgtcaggt cgagatagtt ccaggtgccg ccgagctgtt cgtcgccgcg gttgttgacg
      901 aagtaggtgc ggaacacgcg cgcgccgtcg cggaagaaaa cgttcgtgcc gtgccactcg
      961 tccacaccga aatcggcatc gaagccgtcg gtgatggtgt accacgggaa ttgccagccc
     1021 atgcgttcct tgacccgttg gatatccttc tgcggcgcgc gcgacgcgaa cgcgagcgtc
     1081 gtgtcgcgcg cgttcagatg gaccggatcg gcgacttgat cggcaatcat ggagcagccg
     1141 acgcaagcgt gctcgggcca gccgatgacg ccgggctcca ggaacgcgcg atagacgatc
     1201 aactggcggc gcccgtcaaa taggtcgagc agggtcgcct tgccgttcgg tccctcgaat
     1261 gcgtagtgct tctccaccgc gagccacggc attcgccggc gctccgcggc cagtgcgtcg
     1321 cgagcgcgcg tcagctgttt ttcttttacg agcagccgtt cgcgggctgc ttcccactct
     1381 tggggtgaga cgatcggcgg tgttttcatt gcgaactcct cgcttggggt ccggcgatga
     1441 ccgcatcggc ctgcgccatg atcgcggtcc agccttcttg gtgccggtcg cgggagtcgg
     1501 cgctcgcgaa ctgctcgtgg gtgaggacga gttccgtccc gccgtcgact tcgttgaact
     1561 cgagcgtcag cagcgtgtcg tactcgtctt cgggtttgtc ttcttcccag cgccaagtca
     1621 tcgagagccg ctcgggcggc cgcacttcgc ggtacacccc gcccacgttc cagcgctcgc
     1681 cgtcgggcat ttgcatcacg atggtatagc tgccgccggt tctgacgtcc atgcgggcct
     1741 cgggcaccgt gacgtcgccg gttccgagaa actgtatcgc cagctccggt ttcgtccagg
     1801 cgtcgaaaac gcgttgccgc ggagccgcgt agacgcggcg caatacgagg gccggggcgg
     1861 cggccggcgt gcgagtatcg ttcatttttt cttctttcgt gtaggcggcg ctgcgagaag
     1921 atgttcgagg cgatcgagcg cggcgctcca gaaggtgcgt tgtttgtcga tccaggccga
     1981 ggcggctttc atggcgtcgg gtgaaagcgt gcagcggtgc acgcgaccca agacttgccg
     2041 ctcgagcagg ccggacgctt cgaggatgcg cacgtgcctg gaaatcgccg gctgtgagat
     2101 cgagaaggcg gatgcgatct cgccgaccga ctgcggcccg cgtgccagcc gctcgacgat
     2161 ctgcctgcgg accggatcgc ccagagcgga gaacacggca tcgagcctct caggattatt
     2221 aaccatatcg ttatataact atactgttaa gcgacgcgga ttgtcaaggc cttggctgcc
     2281 catccgattc cgccggcatt cccctcggta actgt
//
LOCUS       DASUCJ010000368         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_722182, whole genome shotgun sequence.
ACCESSION   DASUCJ010000368 DASUCJ010000000
VERSION     DASUCJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722182"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..>1234
                     /gene="metK"
                     /locus_tag="VFU90_04455"
     CDS             313..>1234
                     /gene="metK"
                     /locus_tag="VFU90_04455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5479058.1"
                     /translation="MPERFLFTSESVTEGHPDKIADQISDGVLDAILAEDPGARVACE
                     TLVTTGLACVAGEITTTTYVSIPDIVRSTIADIGYTDATFGFDAKTCAVMSTIDRQSP
                     DIAMGVDTGGAGDQGMMFGYATDETPELMPMPIQLAHALTRALAERRNCGDLPWLRPD
                     GKAQVTVEYENGRPVRVPTVVVSTQHSDAISSDDVRAAIIGNVIVPTLPREMFDPRRA
                     AIHVNPTGRFVVGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRCAAYAA
                     RWVAKNIVAAGLAHKCELQIAYAIGVREPVS"
BASE COUNT          225 a          414 c          388 g          207 t
ORIGIN      
        1 agaggagcct gtgtggcctc tgtgcgagac gacaacctca tttctcttgg ttttcaacaa
       61 agaaaacgat cgctgaccac ccgcgacgca ctgtgatccg aagccgagcg atccgttcaa
      121 tccgtataat ccgcccaatc cgcgttcccc agagcgagga tgcccgcccg agcagtccgg
      181 cgttgtccgg ctccgtcccg gtgtgtccgc cttcgcgggt tgtctgaccc ccagtcaatc
      241 ggtatcattc gaccgtcatt tcactctcga tcagacgctg agccgcttgc gacggcagcg
      301 ctggagaatc gtgtgcccga gcgcttcctc ttcacctcgg agtccgtaac cgagggacac
      361 ccggacaaga ttgccgacca gatctccgat ggagtgctgg acgcgattct cgccgaagat
      421 ccgggtgcac gcgtagcctg cgagacgctc gtcacgacgg ggctcgcctg cgtggccggc
      481 gagatcacca ccacgacgta cgtctccatc cccgacatcg tccgctcgac cattgccgac
      541 atcggttaca ccgatgcgac gttcggcttc gacgccaaga cgtgcgcggt gatgagcacg
      601 atagaccggc agtcgcccga catcgccatg ggcgtcgaca ccggcggtgc aggcgatcaa
      661 ggcatgatgt tcggctatgc aactgatgaa acgcccgagc tcatgccgat gcccatccag
      721 cttgcgcacg cgctcacgcg cgcgttagcc gagcgtcgca actgcggcga tctgccgtgg
      781 ctcaggccgg atgggaaggc acaggtcacc gtcgaatacg agaacggacg gccggtgcgc
      841 gtgccgacgg tggtcgtgtc cacacagcac tctgatgcaa tttcgagcga cgacgtgcgc
      901 gcggcgatca tcggcaacgt gatcgtgccc acgctgccgc gcgagatgtt cgacccgcgg
      961 cgcgccgcga tccacgtcaa ccccacggga cggttcgtgg tcggcggacc gcagggcgat
     1021 gccggcctaa cgggacgcaa gatcatcgtc gacacctacg gcggcgccgc gcgccacggc
     1081 ggtggcgcgt tcagcggcaa ggatccctcc aaggtcgacc gatgcgccgc gtacgccgcg
     1141 cgctgggtcg ccaagaacat cgtcgcggct ggactcgcgc ataagtgcga gctgcagatc
     1201 gcgtatgcga tcggcgtgcg cgagcccgtg agcg
//
LOCUS       DASUCJ010000369         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_263804, whole genome shotgun sequence.
ACCESSION   DASUCJ010000369 DASUCJ010000000
VERSION     DASUCJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263804"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFU90_04460"
     CDS             complement(<1..634)
                     /locus_tag="VFU90_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEU5479059.1"
                     /translation="MPAVARGQTDFFVEEYVRPMMRFAQRCAVALAVALPAAVPASAQ
                     ITQHEYAARRDSLAARLDSGIVIAFGARTPVTDFGPFYQLASFRYLTGFQQPDAAFVM
                     VIREGRQTTTLFTTPVSARLAFYYGFRPDSATIAREGGLTARPLAALPSVMDSLAATG
                     LPVYAIRDVADDDFAAQDSLTRGQQFMKSFAAAHSSLTVKDAEPIVDQLRA"
     gene            complement(689..1258)
                     /locus_tag="VFU90_04465"
     CDS             complement(689..1258)
                     /locus_tag="VFU90_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479060.1"
                     /translation="MLAGGTRTHDTILIPRPSKGKHMITIPTLTTDRLILRAFEQRDL
                     DQYAPIVGDPEVTKHLGDGHALSREEAWRQMAVILGHWMLFGYGLWAVEERATGALIG
                     RIGCQQPEGWPGFEIAYTLGRSYWGRGYAREGAAAALAFAREVLGRTSIISVIRPANA
                     GSIRVAQSLGATLDGEMEFFGGPALIYRY"
     gene            1279..2688
                     /locus_tag="VFU90_04470"
     CDS             1279..2688
                     /locus_tag="VFU90_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEU5479061.1"
                     /translation="MTTTSRESGASPFTDVPPGDSVDGGTSHGFFGHPRGLSTLFFTE
                     MWERFSYYGMRAFLILYMVHALGFNDKHAGSVYGTYTASAWAAAIVGGIIADRWLGQY
                     RSVLYGGIIIALGHFTLAFHALSFFYAGLALIVIGTGLLKPNVSGIVGSLYEPNDARR
                     DAGFSIFYMGINLGAFIGPLIAGYLAQKVDWHLGFACAGIGMTFGVVQYVLGRDRLKP
                     AVERLERQRRESHAAARERKQTDVDARPTEPMFWGFTKVEWKRLAAMGVFFVFAAVFW
                     GAYEQAGSTLNLFGDRYSNTTILGFTFPSSWFVSVQAMFVIILAPAFAWLWIKLGRLE
                     PSTPTKFAIGLLFTGCSFLLLMIPARHLQASPGIRVSPFWLIGCYFIQELGELSLSPV
                     GLSVFTKLSPVKIVGLMLGVWFLADSVGNKIAGYAAGFISSAPLPDLFGTVAAVCLIA
                     SFIAFLMIKPVKSLMGGIH"
BASE COUNT          442 a          869 c          878 g          500 t
ORIGIN      
        1 gcgcgcggag ctggtcgaca atcggctccg catccttcac cgtcagcgac gaatgcgcag
       61 cggcgaacga cttcatgaac tgctgcccgc gcgtgaggct gtcctgggcg gcgaaatcgt
      121 cgtcggcgac gtcgcggatc gcatacacgg gaagtccggt cgcggcgagc gaatccatca
      181 ccgatgggag cgcggcgagc ggcctcgccg ttaggccgcc ctcgcgcgcg atcgtcgccg
      241 aatcgggccg aaacccgtag tagaacgcca agcgcgcgct cacgggcgtc gtgaagagcg
      301 tggtcgtttg ccgtccctcc cggatcacca tcacgaacgc ggcatccggc tgctggaacc
      361 ccgtgaggta gcggaaggat gcaagctgat aaaacggccc gaaatccgtg accggcgtgc
      421 gcgcgccgaa ggcgatgacg atgccgctgt cgagccgcgc cgcgagcgaa tcgcgccgcg
      481 cggcgtactc gtgctgcgtg atctgcgcgg acgcggggac ggcggccggc aacgccaccg
      541 cgagcgcgac cgcacaacgt tgggcgaaac gcatcatggg acgcacgtat tcctcgacga
      601 aaaagtcggt ctgtccgcga gcaacggccg gcaagtggtc aggactatag cgcggcgccg
      661 gacaagcggt aatccccgcg acgaacgttc aataccgata gatcagcgct ggtccgccga
      721 agaattccat ctcgccgtct aacgtggcgc cgagggattg cgcgacgcga atcgagcccg
      781 cgtttgccgg ccgaatcacg ctgatgatgc tcgtgcgccc cagaacctcg cgcgcgaacg
      841 cgagcgccgc cgcggcgcct tcccgcgcgt agccgcgccc ccaatatgat ctgccgagcg
      901 tgtacgcgat ctcgaagccc ggccaccctt cgggctgctg gcagccgatc cgtccgatga
      961 gcgcaccggt cgctcgctcc tccaccgccc acagtccgta gccgaacaac atccaatgac
     1021 cgaggatgac ggccatctgc cgccacgctt cctcgcgcga caatgcgtgt ccgtcgccga
     1081 ggtgcttcgt cacctcggga tcgccgacga tcggcgcata ctggtcgagg tcgcgttgct
     1141 cgaacgcgcg cagaatgaga cggtcggtcg tgagcgtggg aatcgtgatc atgtgcttgc
     1201 ccttggacgg tcgcgggatg agaatggtgt cgtgagtccg agtgccgccc gccagcatct
     1261 cctgtccccg gagtcctcat gacgacaacg agccgcgaga gcggagcctc cccgtttacc
     1321 gatgtgccgc cgggcgactc cgtcgatggc ggtaccagcc acgggttctt cgggcatccg
     1381 cgcggtttgt cgacgctgtt cttcacggag atgtgggagc gcttcagcta ttacgggatg
     1441 cgcgcgtttt tgatcctgta catggtgcac gcgctcggct tcaacgacaa gcatgccggc
     1501 tcggtgtacg gcacgtacac cgcgagcgcg tgggcggcgg cgatcgtcgg cggcatcatc
     1561 gctgatcgtt ggctcggaca atatcgaagt gtgctctatg gcggaatcat catcgcgctc
     1621 ggtcacttca cgctggcgtt tcacgcgctg tcgttcttct acgccgggct ggcgctcatt
     1681 gtcatcggca cggggctgct caagcccaac gtgagcggaa tcgtcggctc gttgtacgag
     1741 cccaacgacg cgcgccgcga cgccggcttc tcgattttct acatgggcat caacctcggc
     1801 gccttcatcg ggccgctgat cgccggatat ctcgcgcaga aggtcgactg gcacctcggc
     1861 ttcgcctgcg cggggatcgg gatgacgttc ggtgtcgtgc aatacgttct cggacgagac
     1921 cggctcaagc ccgccgtgga acgactcgag cgccagcgcc gcgagtcgca tgcggccgcg
     1981 cgcgagcgaa agcagacgga cgtcgacgcg cgcccaacgg agccgatgtt ctggggattc
     2041 accaaagtcg aatggaagcg tctcgccgcg atgggtgtgt tcttcgtgtt cgcggccgta
     2101 ttctggggcg cgtacgagca ggccggctcg acgctgaacc tgttcggcga ccggtacagc
     2161 aacacgacga tcctcgggtt cacgtttccg tcgagctggt tcgtgtcggt gcaggcgatg
     2221 ttcgtgatca ttctggcgcc ggcattcgcg tggctctgga tcaagctcgg gcggctcgag
     2281 ccatcgacgc cgacgaaatt cgccatcggc ctgctcttta cgggatgctc gtttctgctc
     2341 ctcatgattc cggcgcgcca cctgcaggcg tcgccgggca tccgggtgag tccgttctgg
     2401 ctgatcggct gctacttcat ccaggagtta ggcgagctgt cgttgtcgcc ggtggggctc
     2461 tccgtgttca cgaagttgtc gccggtcaag atcgtcgggc tcatgctcgg cgtgtggttc
     2521 cttgcagatt ctgtaggaaa caagatcgcc gggtacgcag ccggtttcat ctcgtcggcg
     2581 ccgctccccg acctcttcgg caccgtggcg gcggtgtgcc tcatcgcatc gttcattgcg
     2641 ttcctgatga tcaagcccgt caagtcgctc atgggcggca tacactaac
//
LOCUS       DASUCJ010000370         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1517107, whole genome shotgun sequence.
ACCESSION   DASUCJ010000370 DASUCJ010000000
VERSION     DASUCJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517107"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFU90_04475"
     CDS             complement(<1..284)
                     /locus_tag="VFU90_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479062.1"
                     /translation="MSILNDTKLEAFLAALHAQSEGQSGAIDAYFARRIKEGTLSWDG
                     LDAETTAFFRDKMVALEKDKAEFCYALCRALGAKRVVECGTSFGVSTLYLA"
     gene            complement(281..667)
                     /locus_tag="VFU90_04480"
     CDS             complement(281..667)
                     /locus_tag="VFU90_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5479063.1"
                     /translation="MKNASIGYINLAVTDFTRSLAFYRDVLALPLLFVDDAFQFAAFS
                     AGSVRFAVVGGADSKKMREARGGDFHTGIGFCVPDVDAAHRELAAKGVRFTMNPSKQP
                     WGGYMALFADPDGNIFYLDTPPEPPR"
     gene            complement(719..>1200)
                     /locus_tag="VFU90_04485"
     CDS             complement(719..>1200)
                     /locus_tag="VFU90_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5479064.1"
                     /translation="RRVPNGLQRPLTEERRMLVKNILGEKGREVVSITSDSTLSEATR
                     LLARRRIGAVVVRDETGALAGILSERDVVHAVADGSVSSLARPVSLFMTRAVATCCET
                     DSIEELMEMMTIGRFRHVPVVDNGMITGIVSIGDVVKTRIAETVQEAASLREYITAA"
BASE COUNT          226 a          386 c          377 g          211 t
ORIGIN      
        1 gccaggtaga gcgtcgagac gccgaacgag gtgccgcatt cgacgacgcg tttggcgccg
       61 agcgcacggc acagcgcata gcagaactcc gccttgtcct tttccagcgc caccatcttg
      121 tcgcggaaga acgccgtcgt ctcggcatcg agcccgtccc agctgagcgt gccttccttg
      181 atgcgacggg cgaaataggc gtcgatcgcg ccgctttggc cttccgactg ggcatgcagc
      241 gcggcgagga aggcttccag cttcgtatcg ttcagaatgc tcatcgtggc ggctcgggcg
      301 gcgtgtcgag atagaagatg ttgccgtcgg gatcggcgaa aagcgccatg tagccgcccc
      361 agggctgctt ggacggattc atggtgaaac gcacaccctt ggcggcgagc tcgcgatgtg
      421 cggcgtcgac gtcgggtacg caaaagccga tgccggtgtg gaaatctccg ccacgcgcct
      481 cgcgcatttt tttcgaatcg gcgccgccga cgacggcgaa acgcacgctg ccggcgctga
      541 atgcggcaaa ctgaaacgca tcgtcgacga acaggagcgg cagagcgagc acgtcgcgat
      601 agaaggcgag cgaacgggtg aagtccgtca ccgccagatt gatgtaaccg atcgacgcgt
      661 tcttcacgct ccctcccgcg ccccgagggc catgcgtgcg agttgaaacc ttcgaagcct
      721 aagcggccgt gatgtattcg cgcaaggacg cggcttcctg caccgtttcg gcgatgcgcg
      781 tcttgacgac gtcgccgatc gagacgatgc cggtgatcat gccgttgtcg accacgggca
      841 cgtggcggaa gcggccgatc gtcatcatct ccatgagttc ctcgatcgaa tccgtttcgc
      901 agcacgtcgc cacggcacgc gtcatgaaaa gactgacagg acgcgccagc gatgagacgc
      961 tgccatcggc cacggcgtga acgacatcgc gttcggacaa gatgccggca agcgcgccgg
     1021 tctcgtcgcg cactaccacc gcgccgatgc gacgccgcgc gagcaggcgc gtggcctccg
     1081 atagcgttga atcgctcgtg atcgaaacca cttcgcggcc cttctcgccc agtatgttct
     1141 tgaccaacat gcgtcgctcc tccgtcaacg gtcgttgcaa accgttcggc actcgacgcc
//
LOCUS       DASUCJ010000371         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_324585, whole genome shotgun sequence.
ACCESSION   DASUCJ010000371 DASUCJ010000000
VERSION     DASUCJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324585"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFU90_04490"
     CDS             <1..299
                     /locus_tag="VFU90_04490"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479065.1"
                     /translation="SAIEATIRALAAKTWAASRKTERIGRTVVLKLKTADFHILTRSH
                     TPPLPPQSEREFVEVAASLIARVELPATTLYRLAGVGLSNFREADEFTQPELFN"
     gene            536..>1018
                     /locus_tag="VFU90_04495"
     CDS             536..>1018
                     /locus_tag="VFU90_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479066.1"
                     /translation="MSIPGLVLLAGFCGAAFAAAPYPVTMCPADRLATNLNCTANDVE
                     VASLDVLNGVTTCVAGDPVSLHLQANIHANAQHRYNVGIFMAQDGKTGLLTSANGGSA
                     ACSTFGLPIQTSPPSPFADLDGNVCGDFDNSVLPSQPPPYPLDLGTVTVTCTPDASGY
                     L"
BASE COUNT          183 a          362 c          310 g          163 t
ORIGIN      
        1 tgtccgccat cgaagccacg atccgtgcgc tcgcagcgaa gacctgggcg gcaagccgca
       61 agaccgagcg catcggccgc accgtggtgc tcaagctcaa gaccgcggat ttccacatcc
      121 tcacgcgcag ccacacaccg ccactgccgc cacagtcgga acgcgagttc gtggaggttg
      181 ccgcgtcgct catcgcgcgc gtggaactgc cggcgacgac gctttaccgg ctggccggcg
      241 tggggttgtc gaatttccgc gaggcggacg aattcaccca gccggagctg ttcaactagc
      301 ggtagcacgc cagcaccgcg cccattacgg tgggtgtccc tacccaggca cccgaacgac
      361 gaatgcacgc gggcacggac ggagttgatc tggatcaata ccggcgccgg gcgtcgtccg
      421 atcatcgccg ccaaacgcgg ggagatccgt catgcgcgac tcgaaattga ccaccgaatg
      481 tgcgtcgaca ccaaagtctg gtaccgaagg atccgaacgg cgccggtttc ggcgcttgag
      541 cattcctggc ctcgtcctgc ttgccgggtt ctgcggagcc gccttcgcgg cggcgcccta
      601 tcccgtgacg atgtgccccg cggaccgctt ggccacgaac ctcaactgca cggcgaacga
      661 cgtggaagtc gccagcttgg acgttctcaa cggcgtcacg acctgcgtcg ccggcgatcc
      721 ggtcagcctg catcttcagg ccaacatcca cgctaacgca caacatcgct acaacgtcgg
      781 catcttcatg gcgcaggacg gcaagaccgg cctgctgaca tccgcgaacg gcggcagcgc
      841 ggcgtgctcg acattcggcc tgccgatcca gacgtcgccg ccgtcgccgt tcgccgatct
      901 cgacggcaat gtctgcggcg atttcgacaa cagcgtactg ccgtcgcagc cgccgccgta
      961 tccgctcgac ctcggcacgg ttaccgtcac ctgcacgccg gacgcgagcg gctacctg
//
LOCUS       DASUCJ010000372         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_202837, whole genome shotgun sequence.
ACCESSION   DASUCJ010000372 DASUCJ010000000
VERSION     DASUCJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202837"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VFU90_04500"
     CDS             complement(<1..1511)
                     /locus_tag="VFU90_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5479067.1"
                     /translation="MIETLERVFAAGGPLARALPGFEARPGQIQMAQLVERGILEGAH
                     TIVEAGTGVGKSLAYLVPAIRSGKKVVLSTGTIALQEQLVTKDIPLVQSALKANVRVT
                     LLKGRSHYLCRQKFERLRSDRLVASSQTMQRLWDWAARTQTGDRAELRFVPSGDEWEQ
                     LDADADDCVGEFCERYRDCFYFRKRDEAKYSDLIVVNHALFFLDLAVGGGLLPPYDVA
                     VLDEAHQCERWATDALTSTLSRATVGRMLRKLHRAYDLPATFDAELEEGMRAMESAFA
                     RVPGERYPLAANEGAWAPLEELRDAFYRLENWLFANWHAALKIKPDNDAEAERRRDLA
                     MRAITAHAATIERAGAPADEAIAWVERGDADGRYALNCAPHDVADFLRATLFARTQSV
                     VLTSATISVATPSEDGGHEASFAFLRRSLGIDRAQELVAPSPFDFDRQARLFIAPARL
                     NPKSADFAQLAAPLVEECLDRSSGRAFVLFTSYVRLREVYALLRERLAFPLRL"
     gene            complement(1508..>2035)
                     /locus_tag="VFU90_04505"
     CDS             complement(1508..>2035)
                     /locus_tag="VFU90_04505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5479068.1"
                     /translation="VLQGGQWWRIFTGAFLHGSVLHIGVNMLSLWYLGRFIEYVLGPW
                     RMLFVYVASLVAAGLGVVYFSASMVPTVGASGAIFGLFGALFAVGFKLGKPGMELVKA
                     NLGILILNLIITFTVPAISWHAHVSGLAAGFLLTLLIYAPPRRVQPVVVDAKTGSELE
                     TEYEPPQDRPSQPYP"
BASE COUNT          354 a          742 c          636 g          303 t
ORIGIN      
        1 tgcagccgca gcggaaacgc cagccgctcg cgcagcagcg cgtacacttc gcgcagccgc
       61 acgtacgacg tgaacagcac gaacgcgcgg cccgagctgc gatcgagaca ttcctccacc
      121 agcggcgcgg ccagttgcgc gaagtccgcc gacttcggat tgagccgtgc cggcgcgatg
      181 aacagccgcg cttgccggtc gaaatcgaac ggcgagggcg ccaccagttc ctgcgcgcga
      241 tcgattccca agctgcgccg caagaacgca aacgaggctt cgtggccgcc gtcctccgac
      301 ggcgtcgcaa ccgagatcgt cgcgctcgtc agcaccacgc tttgcgtccg cgcgaacagc
      361 gtcgcccgca ggaagtcggc gacgtcgtgc ggcgcgcagt tgagcgcgta acgcccgtcg
      421 gcgtcgccgc gctccaccca cgcgatcgct tcgtccgccg gcgcaccggc ccgctcgatc
      481 gtcgccgcgt gcgcggtaat cgcccgcatc gccaggtcgc gccgccgctc cgcttccgcg
      541 tcgttgtccg gtttgatctt cagcgccgcg tgccagttcg cgaacagcca gttctccagc
      601 cggtagaacg cgtcgcgcag ctcctccagc ggcgcccacg cgccttcgtt ggcggccagc
      661 ggatagcgct cgccgggcac ccgcgcgaac gccgattcca tcgcccgcat cccttcttcc
      721 agctcggcgt cgaaggtggc cggcaggtcg taggcgcggt gcagtttgcg cagcatccgg
      781 ccgaccgtcg cacgtgagag cgtcgacgtc agcgcgtcgg tcgcccagcg ctcgcactgg
      841 tgcgcttcgt cgagaacggc gacgtcgtac ggcggcagca gcccgccgcc gaccgccaaa
      901 tccagaaaga acagcgcgtg gttgacgacg atcaggtccg agtacttggc ttcgtcgcgt
      961 ttcctgaagt agaagcagtc gcgatagcgc tcgcaaaact cgccgacgca gtcgtcggca
     1021 tcggcatcga gctgctccca ctcgtcgccg ctgggaacga atcgcagctc ggcccggtcg
     1081 ccggtttgcg tgcgcgcggc ccaatcccac agtcgctgca tcgtctgcga cgacgccacc
     1141 agccggtcgg agcgcaagcg ctcgaacttt tgccggcaca agtagtggct gcgccctttg
     1201 agcagcgtga cccgcacgtt cgccttcaac gccgactgca cgagcgggat gtctttggtg
     1261 accagctgtt cctgcagcgc gatcgtgccg gtcgaaagca cgaccttctt gccgctgcga
     1321 atcgccggca cgagatacgc gagcgatttg ccgacgccgg taccggcttc gacgatcgtg
     1381 tgcgcgcctt cgaggatccc gcgctcgacc agctgcgcca tctggatttg tcccgggcgc
     1441 gcttcaaacc ccggcagcgc gcgcgccagc gggccgccgg ccgcgaagac gcgctccaac
     1501 gtctcgatca cggatacggc tgcgacggac gatcctgcgg cggttcgtac tcggtttcca
     1561 gctcgctgcc ggtcttcgca tccaccacca ccggctgcac gcgccgcggc ggagcgtaga
     1621 tcagcagcgt cagcaagaat ccggcggcca ggccggagac gtgcgcgtgc cacgaaatcg
     1681 ccgggacggt gaacgtaatg atcaggttca gaatcaagat gccgaggttg gccttcacga
     1741 gctccatccc cggcttgccg agcttgaacc cgaccgcgaa gagcgcgccg aacaaaccga
     1801 agatcgcacc gctcgcgccg accgtcggca ccatcgacgc gctaaagtac acgaccccga
     1861 gcccggccgc caccagcgac gcaacgtaaa cgaacagcat ccgccacggc ccgagcacgt
     1921 attcgataaa gcgcccgaga taccacagcg acagcatgtt gacgccgatg tgcagcacgc
     1981 tgccgtgcag gaacgcgccg gtgaaaatcc gccaccactg gccgccctgc agcac
//
LOCUS       DASUCJ010000373         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_737694, whole genome shotgun sequence.
ACCESSION   DASUCJ010000373 DASUCJ010000000
VERSION     DASUCJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737694"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFU90_04510"
     CDS             complement(<1..713)
                     /locus_tag="VFU90_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU5479069.1"
                     /translation="MRRLVLALALVVLARPLLAQDLSCSGAGPGNLQIRSLSFEGNDH
                     FSDSQLSNAIVTTPSSWFRRVLHIPIGAEHCLDTLEVARDALRLRLLYRQHGYYHTQA
                     TPHISPNGTSAVSVMFQIVEGPPVRIDSLTVAGLDSVSDRLRERLIRPLDRLRGGIYD
                     RDTLLAVIDTTVTRLLNNGYAHATDPLRDIEVDEAANRAVVKLTFLPGKIAHIGKIDI
                     QAEANKQGEKPAIDTETVH"
     gene            complement(829..>1560)
                     /locus_tag="VFU90_04515"
     CDS             complement(829..>1560)
                     /locus_tag="VFU90_04515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5479070.1"
                     /translation="CRAVKLNPFTARIPVLMLTARGDVEHKVEAFEAGADDYLAKPFD
                     PRELRARIVALLRLVRREGDRNPTSGLPGGQAIEEEIARRAKLRERFAICYIDLDNFK
                     PFADTFGFTIADVVIRDMGHAIRNAVDVVGSHEDFVGHIGGDDFIVVTTEQHAEAVAR
                     ECAIRFVEVAGRAVGDEALRRGHFMGIDREGRAREFPIARLSTAILFVDPDRWISVSH
                     IGSLAAEMKRRAKQRGPGTILVQAV"
BASE COUNT          276 a          543 c          470 g          271 t
ORIGIN      
        1 tcgtgtacgg tctccgtgtc gatcgcaggt ttctcccctt gtttgttggc ctcggcctgg
       61 atgtcgatct tcccgatgtg cgcgattttg ccggggagga acgtgagttt gaccaccgcc
      121 cggttggcgg cttcgtccac ctcgatgtcc cgcagtggat cggtcgcgtg tgcgtagccg
      181 ttgttcagca gccgcgtgac ggtggtgtcg atcacggcga gcagcgtatc gcgatcgtaa
      241 atgccgccgc gcagacggtc gagtggtcgg atgagacgct cgcgcagccg gtcggacacc
      301 gagtcgagac cggccaccgt cagcgaatcg atgcgcacgg gcggcccttc cacgatctga
      361 aacatcaccg acaccgcgct cgtgccgtta ggcgagatgt gcggcgtcgc ctgcgtgtgg
      421 tagtagccgt gctggcggta cagcagccgc agccgcagcg cgtcgcgcgc cacctcgagc
      481 gtgtccagac agtgctcggc gccgatcgga atgtgcagca cccgccgaaa ccagctcgac
      541 ggcgtggtga caatggcgtt gctcagctgg ctgtcggaga agtggtcgtt cccttcgaac
      601 gacaagctcc gaatctgcag attcccggga ccggcgccgc tacacgagag gtcttgcgca
      661 agcagtggtc gcgcgagcac gacgagcgcg agcgcgagta cgagccgccg catcaggcgt
      721 gagacgcccc gacgacaaaa aacgactgaa cgaaaacggc ccgccatgat cggcggccga
      781 cgcgcgttgc tcgcatgttc gctctcgttg ccttacgatc cgcgttcact acaccgcctg
      841 cacgagaatc gtgcccggcc cgcgctgctt cgcccgccgc ttcatctcgg cagccagcga
      901 cccgatgtgg ctcaccgaaa tccaccggtc cggatccacg aagagaatcg ccgtcgacaa
      961 ccgcgcgatc ggaaactctc tcgcacgtcc ttcgcgatcg atacccatga agtgcccgcg
     1021 ccggagcgcc tcgtcaccca ccgcacgacc cgccacctcg acgaaccgaa tcgcgcactc
     1081 gcgcgcgacc gcctcggcgt gctgttccgt cgtcacgacg atgaaatcgt cgccaccgat
     1141 gtgcccgacg aaatcctcgt ggctccccac cacgtccacc gcgttgcgaa tcgcgtgacc
     1201 catgtcccga atcaccacgt ccgcgatcgt gaaaccgaac gtgtccgcaa acggcttgaa
     1261 gttgtcgaga tcgatgtagc agatcgcgaa tcgttcgcgc agcttcgccc gccgcgcgat
     1321 ctcttcctcg atcgcctgac cgccgggcag cccactcgta gggttgcgat cgccttcgcg
     1381 ccgcaccaat cgcagcaacg ccacgatgcg cgcccggagc tcgcgcggat cgaacggctt
     1441 cgccaagtag tcatcggcgc ccgcctcgaa tgcttccact ttgtgttcga catcgccgcg
     1501 cgccgtgagc atcagcacgg gaatccgcgc cgtgaacgga ttcaacttca cagcgcgaca
//
LOCUS       DASUCJ010000374         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1180773, whole genome shotgun sequence.
ACCESSION   DASUCJ010000374 DASUCJ010000000
VERSION     DASUCJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180773"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFU90_04520"
     CDS             <1..351
                     /locus_tag="VFU90_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479071.1"
                     /translation="VFALSACATGGGPRGGAGHDPNTITAAEVESSHESNAYDVVRSI
                     RPNMLTTRGQSTFINSDPGIVVFLNGTQYGDIQSLRRINASDIDQIHFYSASEASLRH
                     GTGYPEGVIEVTTK"
     gene            373..>1106
                     /locus_tag="VFU90_04525"
     CDS             373..>1106
                     /locus_tag="VFU90_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479072.1"
                     /translation="MSLADVLRTALGQIRANKLRSFFTLLGIIVSVGFLVAVVAIIQG
                     MNAYVKENVADAIVGVNTFQVRRIPIKIGLFTDDELRAIQKRPKITMADAQVVARAVP
                     GADAVALQSGFPTPATQVAWHGRTLENVFVFGVTAPYQTVQDYRFRSGSPITDVDVRE
                     RRQVIAIGADVADKVFGDADPIGHTVRIMGARFTIVGVIAPKGRVLGQSFDSFVIMPI
                     TSFELLYGRRATTTISVKMPNAESIPS"
BASE COUNT          195 a          375 c          349 g          187 t
ORIGIN      
        1 gtcttcgcgc tgtccgcctg tgctaccggc ggcggcccgc gcggcggtgc gggccacgac
       61 cccaacacga tcacggcggc cgaggtcgag agcagccacg agagcaatgc ctacgacgtg
      121 gtccgctcga tccgccccaa catgctgacc acgcgcggcc agtccacgtt catcaactcc
      181 gaccccggta tcgtggtgtt cttgaacggc acgcagtacg gcgacatcca gtcgctgcgc
      241 cggatcaacg ccagcgacat cgatcagatc cacttctact cggcgagcga ggccagtctg
      301 cgccacggaa ccggctatcc cgagggcgtg atcgaagtga cgacgaaata ggaaaggcgt
      361 tatgttgcat cggtgagtct cgccgatgtc ctgcgcaccg cgctcgggca gatccgggcg
      421 aacaagctgc ggtcgttctt caccctgtta ggcatcatcg tgtccgtcgg ctttctcgtg
      481 gccgtggtcg cgatcataca ggggatgaat gcgtacgtga aagagaacgt ggccgacgcc
      541 atcgtcggcg tgaacacgtt ccaggtgcgc cgcattccca tcaagatcgg gctcttcacc
      601 gacgacgagc tgcgcgcgat ccagaagcgg cccaagatca ccatggccga tgcgcaggtg
      661 gtcgcgcgcg ccgtgcccgg ggccgacgcc gtcgccttgc agagcggatt ccccactccg
      721 gcgacgcagg tcgcctggca cggccgcacg ctcgagaacg tattcgtctt cggcgtgacc
      781 gcgccgtacc agacggtgca ggattaccgc tttcggagcg gcagccccat caccgacgtg
      841 gatgtgcgcg agcgccgcca ggtgatcgcc atcggcgccg acgtggcgga caaggtgttc
      901 ggcgacgccg atccgattgg ccacaccgtg cgcatcatgg gcgcacggtt cacgatcgtc
      961 ggcgtcatcg cgcccaaggg ccgtgtcctc ggccaatcct tcgactcgtt cgtgatcatg
     1021 ccgatcacga gcttcgagct gctctacggc cggcgcgcga cgaccacgat ctcggtcaag
     1081 atgcctaacg cagagtcgat cccgtc
//
LOCUS       DASUCJ010000375         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_981995, whole genome shotgun sequence.
ACCESSION   DASUCJ010000375 DASUCJ010000000
VERSION     DASUCJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFU90_04530"
     CDS             complement(<1..299)
                     /locus_tag="VFU90_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479073.1"
                     /translation="MFTIDLTGRRALVAGVADDAGYGFAIAKALAECGATVVVGTWPP
                     ALTIFNNLLERGKMDASRRLSSGAMMEFERIYPLDAAFDTMADVPDEIRTNKRYA"
     gene            614..>1481
                     /locus_tag="VFU90_04535"
     CDS             614..>1481
                     /locus_tag="VFU90_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5479074.1"
                     /translation="MPNKLLTFPISEHTLANDLRVIVVPTGLPDVVSVQIPVQAGSRN
                     EVEPGKTGFAHFFEHMMFRGTEAYPPARYQEVLTRAGARSNAYTTDDYTNYHATFSRD
                     DLARVLEIEGDRFQRLRYPEEDVKTEARAVLGEYNKNSADPTEKLFEVMRDHAYTTHT
                     YKHTTMGFLRDIEDMPNQFDYSRLFFDRWYRPEYTTILVAGDVSASDVLPLVERYWGA
                     WPRGSYRADVPRDPAPSGPVYAHVPWDTPTLPWVTTAFRAPAFDATSRAFAATDLMVD
                     LWFGRTSALYRRL"
BASE COUNT          274 a          494 c          479 g          234 t
ORIGIN      
        1 ttcgcgtacc gtttgttggt ccggatttcg tcggggacat cggccatcgt gtcgaacgcg
       61 gcgtcgagcg gatagatgcg ctcgaattcc atcatcgcgc cgctcgacaa gcggcgcgac
      121 gcatccatct tgccgcgctc caggagattg ttgaagatgg tgagcgcggg cggccacgtg
      181 ccgacgacga cggttgcacc gcattccgcg agcgccttcg cgatcgcgaa gccgtagccg
      241 gcgtcgtcgg ccacgcctgc gaccagtgcg cgccggcccg tgagatcgat ggtgaacata
      301 cgcggaggaa atagcgattc gcggtggcgc ccacaagggc ggataaaagg cggacacggc
      361 gcggacaagg acggacaacg ccggaccgct cggccgcagg ggaagacgtt ccggcagggg
      421 cggccaaggg cggatgagga acgcgagcat gcgtcggccg tagggcgtgg cctaacggat
      481 aaacgagccg taccttgttg aacaagaatg gccaagaagg tcgtgctggc tccgaggtga
      541 cctcatcacg tcgaggccga tcgggcgtgg gccgttactc gcacgcgatg gagcccgcgc
      601 ttatcctacg gacatgccga acaagctcct cacctttccg atttcggaac acaccctcgc
      661 gaacgatctt cgcgtgatcg tcgtgccgac cggcctgccg gacgtcgtgt ccgtgcagat
      721 tcccgtgcaa gcgggctcac gcaacgaagt cgagcccggg aagacgggct tcgcgcattt
      781 cttcgaacac atgatgttcc gcggcaccga ggcgtatcct ccggcgcgct atcaggaagt
      841 gctcacgcgc gcgggcgccc ggtccaacgc gtacaccacc gacgactaca cgaactacca
      901 cgcgaccttc tcgcgcgacg atctggcgcg cgtgctcgag atcgaaggcg accggttcca
      961 gcgcctgcgc tatcccgaag aggacgtcaa gaccgaagcg cgcgcggtgt taggcgaata
     1021 caacaagaac agcgccgacc ctaccgaaaa gttgttcgag gtgatgcgcg accacgcgta
     1081 caccacgcac acctacaagc acactacgat gggcttcctg cgcgacatcg aggacatgcc
     1141 caaccagttc gactactcga ggctgttctt cgaccggtgg tatcggcccg aatacacgac
     1201 gatcctcgtc gccggcgacg tgtcggcgag cgacgtgctt ccgttagtcg aacgctactg
     1261 gggcgcctgg ccgcgcggca gctatcgcgc cgacgtgccg cgcgacccgg cgccctcagg
     1321 ccccgtgtac gcgcacgtgc cgtgggacac gccgaccctg ccgtgggtca ccaccgcgtt
     1381 ccgcgcgccc gcgttcgacg cgacgagccg cgcgttcgcg gccaccgatc tcatggtcga
     1441 tctctggttc ggccgcacgt cggcgctgta ccgccgcctc g
//
LOCUS       DASUCJ010000376         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_80430, whole genome shotgun sequence.
ACCESSION   DASUCJ010000376 DASUCJ010000000
VERSION     DASUCJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80430"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFU90_04540"
     CDS             <1..423
                     /locus_tag="VFU90_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5479075.1"
                     /translation="GTRALLESLRERGMRLYLASGTDEEYMKHEADLLGVTPYFDGGV
                     YGAIDDYKNFSKGILVRRILESAEFRGSDFLGFGDGFVEIEEVKKVGGTAVGVATAEP
                     ECRAIDDWKRQRLIGVGADMIVPNYLELPILESALFPA"
     gene            428..>1017
                     /locus_tag="VFU90_04545"
     CDS             428..>1017
                     /locus_tag="VFU90_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479076.1"
                     /translation="MTSKYELFDRSRLKIRPLDERQHDLSLDNWLALDDAAPAFTHPD
                     LGVVASRLAAARESGVTRMLVMGAHLLRAGVNRHIIDLLNRGFINHVAMNGAGVIHDY
                     ELARIGATTESVERYIASGEFGLWAETGELNEWIREAAAFGLGLGENAGRRIAASDYP
                     HRDLSVLAAAYRLGIPATVHVGVGYDIIHEHPNCDGA"
BASE COUNT          197 a          312 c          322 g          186 t
ORIGIN      
        1 ggcacgcggg cgctgctcga gtcgctgcgc gaacgcggca tgcgcttgta cctggcgagc
       61 ggtaccgacg aggagtacat gaaacacgag gcggacctgc tcggggttac gccttacttc
      121 gatggcggag tgtacggcgc catcgacgat tacaagaact tttcgaaagg aatcctcgtt
      181 cgccggattc tggagtcggc cgaattccgc ggcagtgatt ttctcggctt cggcgacggc
      241 ttcgtagaga tcgaagaggt aaagaaagtg ggcggcaccg cggtcggcgt tgcgactgcc
      301 gagccggagt gccgggcgat cgacgattgg aaacgccagc ggctcatcgg cgtcggcgcg
      361 gacatgatcg tccccaatta cctcgaactc ccgatattgg aatccgcgct ttttccggcc
      421 tgaaccgatg acctcaaaat acgaactgtt cgaccgttca cggctgaaga tccgtccgct
      481 cgacgagcgg cagcacgatc ttagcctcga caactggctc gcgctggacg atgccgcgcc
      541 ggcgttcacg catcccgatc tcggcgtcgt cgcatcgagg cttgcagcgg cgcgtgaaag
      601 cggagtgacc cgcatgctgg tgatgggcgc acacctgctt cgcgccggtg tgaatcgtca
      661 catcatcgac ctgctcaatc gcgggttcat caaccatgtg gcgatgaacg gcgccggcgt
      721 cattcacgat tacgaactcg cgcgcatcgg cgcgaccacg gagagcgtcg aacgctacat
      781 cgcctcgggc gaattcggat tgtgggccga aacgggcgaa ctgaatgaat ggatccgcga
      841 ggccgctgca ttcggactcg gcctcggcga gaacgccggc cggcgaattg ccgcgagcga
      901 ctatcctcac cgcgatctga gcgtgctggc cgccgcatac cggctcggca ttccagccac
      961 cgttcacgtc ggcgttggat atgacatcat ccacgaacat ccgaactgcg atggcgc
//
LOCUS       DASUCJ010000377         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_263848, whole genome shotgun sequence.
ACCESSION   DASUCJ010000377 DASUCJ010000000
VERSION     DASUCJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263848"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFU90_04550"
     CDS             <1..812
                     /locus_tag="VFU90_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HEU5479077.1"
                     /translation="QLARSEALSAFGSGDVYVEKYLSRPRHIEFQIVGDKHGRVIHLG
                     ERDCSVQRRHQKLIEEAPSPAVGPALRERMGHDAVRGAKAIDYVGAGTIEMLLDEDGS
                     YYFMEMNTRIQVEHGVTEMLTGVDLVKEQLRVAAGEPLSIAELPPLRGHVIEVRVNAE
                     DPSRNFQPSPGRIDVFHPPGGPGVRIDSHVYAGYTVPPFYDSLLAKLICQGRDRAEAV
                     KRVQVALESFVLEGVTTTIPFLARVMQNAAFQAGQVDTKFLEREASLLREP"
     gene            826..>1129
                     /locus_tag="VFU90_04555"
     CDS             826..>1129
                     /locus_tag="VFU90_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU5479078.1"
                     /translation="MLFGPSMLAPATVTGRVVAVIDVLRASTTIATALANGARNVVPL
                     ESADAVMTRAKQLERADVRTAGERKMQPIPGFDLGNSPREMTREAVDGKTVLFTTTN"
BASE COUNT          195 a          335 c          404 g          195 t
ORIGIN      
        1 tccaactcgc gcggtccgaa gcgctgtccg cgttcggctc aggcgacgtg tacgtcgaga
       61 agtatctctc gcgacctcgg cacatcgagt tccagatcgt gggcgacaag cacggccggg
      121 tgattcattt aggcgaacgc gattgttccg tgcagcgccg ccatcagaag ttgatcgagg
      181 aagcgccgag ccccgccgtt gggcctgcct tacgggagcg gatgggacac gatgccgtcc
      241 gcggcgcgaa agccatcgat tacgtcggcg ccggcacgat cgagatgctg ctcgacgaag
      301 acggttcgta ctacttcatg gaaatgaaca cgcgcatcca ggtcgagcac ggcgtcaccg
      361 aaatgctcac gggcgtggat ctggtgaagg agcagctgcg cgtggccgcg ggggagccgt
      421 tgtcgattgc cgagctgccg ccgctgcgcg gacacgtgat cgaggtgcgt gtgaatgcgg
      481 aggatccgtc gcgcaatttc caaccgtcgc ccggtcgcat cgacgtgttc catccgcccg
      541 gcggaccggg cgtgcggatc gactcgcacg tctacgccgg ctacaccgtg ccgccgttct
      601 acgactcgtt gctggcgaag ctcatctgtc aggggcgcga tcgcgccgaa gcggtgaagc
      661 gggtgcaggt ggccctcgag agtttcgtgc tggaaggcgt gacgacgacc attccgtttc
      721 tcgcgcgtgt gatgcagaac gcggcgtttc aggccggcca ggtggacacc aagtttctcg
      781 agcgtgaggc gtcgctgttg cgggagcctt gagatgcgaa tcgatgtgtt gttcgggccg
      841 tcgatgctgg cgccggcgac ggtgacggga cgggtggttg cggtgatcga cgtgctgcgg
      901 gcgtcgacca cgatcgcgac ggcgcttgcc aacggcgcac gcaacgtggt acctctggaa
      961 agcgccgatg ccgtgatgac gcgcgccaag cagctcgagc gcgccgacgt gcgcacggcg
     1021 ggagagcgga agatgcagcc gatcccggga ttcgatcttg gcaactcgcc gcgggagatg
     1081 acgcgcgagg ccgtggacgg caagaccgtg ctgttcacga cgacgaacg
//
LOCUS       DASUCJ010000378         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1058600, whole genome shotgun sequence.
ACCESSION   DASUCJ010000378 DASUCJ010000000
VERSION     DASUCJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058600"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VFU90_04560"
     CDS             complement(<1..1030)
                     /locus_tag="VFU90_04560"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HEU5479079.1"
                     /translation="MRAFPVVAGLALAAFAVLQAPESPRSFAAAPASAVVPFTMVSDN
                     MIFPIRINGVRSYAIFDTGGRFVMTPDLARRLNLRTAAKGSVGGMGAGRPAYSVATVH
                     DVAIGDVTLHDQQFVVVPLPYDLTRGGPVPVDVDVGYELLSKYAARIDFKTREITLTE
                     NAAAQRPVGAAAVHLAFSDTTPMVTASIDGLSGRFLADTGSSFAVAFTAPFVAAHRMD
                     SRYPATKTLVTGEGLGGNTYAKLVRTKLLRIGDAEVVRPVAQLSADTAGTLASASYAG
                     VIGIDVLKCFTATFDYARSALYLAPRSPDCPQPFDRTGATVEKTDGNGYAVEGVLANG
                     PAALAGLRA"
     gene            1102..1356
                     /locus_tag="VFU90_04565"
     CDS             1102..1356
                     /locus_tag="VFU90_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479080.1"
                     /translation="MDDAKRLATALNALVKSPQFSNSKARGAARQDPGVDWPATKAHN
                     ADFNPDVDGFESFLMKDGSVCVWVPGQFRYVASATIADRG"
     gene            1596..1952
                     /locus_tag="VFU90_04570"
     CDS             1596..1952
                     /locus_tag="VFU90_04570"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5479081.1"
                     /translation="MKDLGGGAAAAREPRKQGSAAERSELPRMCRNIKTLHNFAPPAT
                     DEEIRASAIQFVRKLSGFNRPSKANEAAFDRAVDRVTDAARDLLASLVTHAPARDREI
                     EAAKARERSKARFATA"
     gene            complement(1959..>2443)
                     /locus_tag="VFU90_04575"
     CDS             complement(1959..>2443)
                     /locus_tag="VFU90_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479082.1"
                     /translation="HAVPHAERVVAELARVTRPGGTLHLIPEDYGMLHFQHRAAFDPA
                     DFWHRAPEQFGAATETDLFIGRDVFTILAKRSFEAIAIDYVVVDPLRVPRETFASILE
                     AWRDGYAESIEELTEFSRESATAYFDAMVANVRDPLGYAVWMVPVVSARKGPATVAPG
                     "
BASE COUNT          426 a          818 c          784 g          415 t
ORIGIN      
        1 cggcccggag accggccagc gccgccggac cgttcgccaa aacgccctcg accgcatatc
       61 cgttgccgtc ggtcttctcg acggtcgcgc cggtccggtc gaaaggctgc ggacagtccg
      121 gcgatcgcgg cgcgaggtag agcgcgctgc gtgcgtagtc gaacgtcgcg gtgaagcatt
      181 tcaggacgtc gatcccgatg acgcccgcat acgacgccga ggcgagcgtg ccggcggtat
      241 ccgccgacag ctgcgcgacc ggtcgcacga cctccgcatc gccgatccgc agcagtttcg
      301 tacggaccag cttcgcatac gtgttgccgc cgagcccttc accggtgacc agcgtcttgg
      361 tcgccggata gcggctatcc atccgatgcg ccgcgacgaa cggcgcggtg aacgcgaccg
      421 caaacgacga acccgtgtcg gctaagaacc ggccggagag tccgtcgatg ctggctgtca
      481 ccatcggcgt ggtgtcgctg aatgcgaggt gaaccgccgc tgcgcccacg ggacgttgag
      541 ctgccgcatt ttcggtgagc gtgatctcac gcgttttgaa gtcgatccgt gcggcgtatt
      601 tggaaagcag ttcgtagccc acgtcgacgt ccaccggaac cgggccgccg cgggtcagat
      661 cgtagggcag cggcaccacc acgaactgct gatcgtgcag cgtgacgtcc ccgatcgcaa
      721 cgtcgtgcac ggttgcgacg gaataagcag gccgacccgc acccattccg ccgacactcc
      781 cttttgctgc cgttcgcagg ttcaagcgtc gagccaggtc gggcgtcatg acgaaccggc
      841 cgcccgtatc gaagatcgcg tagctgcgca cgccgttgat gcgaatcggg aaaatcatgt
      901 tgtcgctgac catcgtgaaa ggcacgaccg cggacgccgg cgcggccgca aaggaacgcg
      961 ggctttccgg cgcttgaagc accgcgaacg cggccagcgc gaggccggcg accaccggga
     1021 acgctctcat gaggcttctc gcttcggcgc gctcgcctgc tcgcctgggc ttccgcgtcc
     1081 cgcgcagcga aggtcgcgac tatggacgat gcaaaacggc tcgccacggc gctgaacgcg
     1141 ctcgtgaagt cgccgcagtt ctcaaacagc aaagcgcgcg gggcggcgcg tcaggatccc
     1201 ggcgtggatt ggcccgcgac caaagcgcat aacgccgact tcaaccccga cgtcgacggt
     1261 ttcgaatcgt ttcttatgaa ggacgggagc gtctgcgtgt gggtgcccgg ccagttccgc
     1321 tacgtcgcga gcgcgacgat cgcggaccga ggctagtctt tcgcgcgccg caactcgaag
     1381 ggcttcggca cgtacgtgta cttttcgccg caaatgcggc acacgttcag cgggcttcca
     1441 aaatgttcgt gcgggggata ttctttcggc gcggctttcg cgcgcttcct gaccgtcgtg
     1501 gcgactgcga tgatttactc gttttcgctc ccgaggctgg gagcagtacg ctttcatggt
     1561 tgaaaagcgt tattttctct cttcggggtt ctgccgtgaa ggacctcggg ggcggcgccg
     1621 cggccgcgcg cgagcctcga aagcagggct ccgccgccga acggtcggag cttccacgta
     1681 tgtgccggaa catcaagacg ctccacaact tcgcaccgcc cgcgaccgat gaagaaattc
     1741 gcgcttcggc gattcagttc gtccggaagt tgagcggttt taaccgcccc tcgaaagcca
     1801 acgaagcggc tttcgatcgc gccgtcgacc gcgtgaccga cgccgcgcgc gacctgctag
     1861 cgagcctcgt cacccacgcg ccggcgcgcg accgggagat cgaagccgcg aaggcacgcg
     1921 aacgttcgaa ggctcgcttc gcaaccgctt gagggcgtct aacccggcgc gaccgtcgcc
     1981 gggcctttgc gcgcgctgac caccggcacc atccaaacgg cgtagccgag cgggtcgcgc
     2041 acgttcgcca ccatcgcgtc gaagtacgcc gttgcggatt cgcgcgaaaa ctcggtgagt
     2101 tcctcgatcg attcggcgta gccgtcccgc cacgcctcga ggatgctcgc aaacgtctct
     2161 cgcggcacgc gcagcggatc gacgaccacg tagtcgatcg cgatcgcttc gaaacttcgt
     2221 ttcgccaaaa tcgtaaagac gtcgcggccg atgaacagat cggtctccgt tgccgccccg
     2281 aactgctccg gcgcccgatg ccaaaagtcg gctggatcga acgccgcgcg gtgctgaaaa
     2341 tgcagcatcc cgtagtcttc cgggatcaga tgcaaggtgc cgccggggcg cgtcacccgc
     2401 gcgagttcgg ccaccacgcg ttcggcgtgc ggcaccgcgt gca
//
LOCUS       DASUCJ010000379         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_951591, whole genome shotgun sequence.
ACCESSION   DASUCJ010000379 DASUCJ010000000
VERSION     DASUCJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951591"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFU90_04580"
     CDS             complement(<1..562)
                     /locus_tag="VFU90_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479083.1"
                     /translation="MAVVGVGTTAYGAFPETDDYGLAARAFRNAVEDCGVDKNAIDGL
                     LCCRIPYYARMGEILGLDPRWTLPMPAHGRMSGMAIVEAMMALESGAADYVALLYANI
                     GRSRRVNYGGEELAGIWDPWGFTSPGAAHALMFRMHMERYGTTTRQLAEVSVAFRRHA
                     QMHPDAVMKKPITIDDHEAARPIVEPL"
     gene            complement(644..>1190)
                     /locus_tag="VFU90_04585"
     CDS             complement(644..>1190)
                     /locus_tag="VFU90_04585"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5479084.1"
                     /translation="VVKSDDTIWFTDPDYGILSDYTGTKAPSEIGVNHLFRFDPKSGE
                     LTPVISTMQKPNGLAFSPDERILYVADTSLSHSQTGHHHIMAWDVLDGRTASNPRVFA
                     VVSPGMSDGFRVDQDGNIWTSAGDGVHCYGPDGVLIGKIRFPEVTTNLTFGGPKRNRL
                     FVTTASCVYAVYVGQKGAQYP"
BASE COUNT          202 a          379 c          406 g          203 t
ORIGIN      
        1 gcagcggctc gacgatcggc cgcgccgctt cgtggtcgtc gatcgtgatc ggcttcttca
       61 ttacggcgtc cgggtgcatc tgcgcgtggc gccggaacgc gaccgagacc tcggcgagtt
      121 ggcgcgtggt cgtcccgtaa cgctccatgt gcatgcggaa catcagcgca tgcgccgcgc
      181 ccggcgacgt gaaaccccac gggtcccaga tgccggccag ttcctcgcca ccgtaattga
      241 cgcggcgtga gcggccgata ttggcgtaga gcagcgcgac gtagtcggcc gcgccgcttt
      301 ccagcgccat catcgcttcg acgatcgcca tgccggacat gcggccatgc gccggcatcg
      361 gcagcgtcca gcgcggatcg agaccgagga tctcgcccat gcgcgcgtag tacgggatcc
      421 ggcagcacag caggccgtcg atcgcgttct tgtcgacgcc gcaatcctcc acggcattac
      481 ggaaggcacg cgccgccaga ccgtaatcgt cggtttcggg gaatgcgccg taggcggtgg
      541 tccccacgcc gacgacagcc acccgattct tcaatcccat catgggcaag ggctccgaaa
      601 aacatggccg catgggccga catggcccga cggcgtactg acgtcagggg tactgcgcgc
      661 ccttctggcc gacatagacg gcgtagacgc aggaggcggt ggtgacgaac aggcggtttc
      721 gcttggggcc gccgaaggtg agattcgtcg tcacctcggg gaagcggatc ttgccgatga
      781 gcacaccgtc cggaccgtag caatggacgc cgtcgccggc gctggtccag atgttgccgt
      841 cctgatcgac ccggaagccg tccgacatgc cggggctgac gaccgcgaag acgcgcggat
      901 tggacgcggt ccggccatcg agcacgtccc acgccatgat gtggtgatgg ccggtctggc
      961 tgtgcgacag cgacgtgtcg gcgacgtaga ggatgcgctc gtccggcgag aaggcaaggc
     1021 cgttgggctt ctgcatggtc gagatgaccg gcgtcagctc gcccgacttg ggatcgaagc
     1081 ggaacagatg gttcacgccg atctcgctcg gtgccttcgt gcccgtgtag tcgctgagaa
     1141 ttccgtagtc gggatcggtg aaccagatcg tgtcgtcgga cttcacgacg
//
LOCUS       DASUCJ010000380         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1180845, whole genome shotgun sequence.
ACCESSION   DASUCJ010000380 DASUCJ010000000
VERSION     DASUCJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180845"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /gene="katG"
                     /locus_tag="VFU90_04590"
     CDS             <1..1655
                     /gene="katG"
                     /locus_tag="VFU90_04590"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEU5479085.1"
                     /translation="PDAWEPDESVYWGPESTWLADERYAGERILENPLAAVQMGLIYV
                     NPEGPNGNPDPLAAAKDIRETFRRMAMNDEETVALIAGGHSFGKTHGAGDPSQVGAEP
                     EAADITLQGLGWKSSFGKGHSGDTIGGGPEVTWTTTPTKWSNNFFESLFKNEWELTKS
                     PAGAKQWVAKNGAAPVPDAHDPSKHHKAGMLTTDLALRYDPIYEKISRRFYEHPDQFA
                     DAFARAWFKLTHRDMGPRARYLGPEVPAEELIWQDPVPKVDHPLVDATDVAALKQRIL
                     DSGLSISELVSTAWASASTFRASDKRGGANGARIRLAPQKDWEVNRPAQLAAVLKKLE
                     EIRHSFNGAQNNGKKISLADLIVLAGCAGVEKAAKNAGFDVTVPFAPGRTDASQEQTD
                     VESFAVLEPVADGFRNYLKDGVAIPAEMMLVDKAQLMTLTAPEMTVLLGGMRVLETNA
                     DGSKHGVFTSRPGALTTDFFVNLLDMHGEWKEKAANLYERQNSKAGTETGTRVDLIFG
                     SNSELRAVAETYGSSDASRKFVDDFVAAWAKVMNLDRYDLAG"
     gene            1674..>2320
                     /locus_tag="VFU90_04595"
     CDS             1674..>2320
                     /locus_tag="VFU90_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479086.1"
                     /translation="MSSRTAAAVVAFAAIILAGCSGATGSRLTPGGAAIPLGAASPTT
                     AYTYYDLGPGPNGKDAYGYGESTTAQAGAYFDHTISCGEDCSAKVYYPLAWSGQGSSP
                     VVLLPQHYVEGWAYGAGGSLIVGTLITNDGGYFGFAHAALWQGSSHTFTDLNPAGNCG
                     SCSPGSNAYGTDGTFIAGSGGSPQHALLWKLAQLKTPVDLNPSGFAYSEAYAVRGT"
BASE COUNT          452 a          733 c          759 g          376 t
ORIGIN      
        1 gtccggacgc ttgggagccg gacgagtcgg tttactgggg tcccgagagc acctggctcg
       61 ccgacgaacg ctacgcggga gaacggatcc tagaaaatcc gctcgcagcc gtgcagatgg
      121 ggcttatcta cgtcaatccc gaggggccga acggcaaccc cgatccgttg gcagctgcga
      181 aagatatccg cgagacgttc cgccgcatgg cgatgaacga cgaagagacc gtcgcgctga
      241 tcgcgggcgg acattcgttt ggcaaaacgc acggcgccgg cgatccctcg caagtcggcg
      301 ccgagccgga agccgcggac attaccctgc aaggcttggg ctggaaaagc agcttcggta
      361 aaggacacag cggggatacg atcggcggcg ggccggaagt gacctggacc acgaccccga
      421 cgaaatggag caacaacttc ttcgagtcgc tcttcaagaa cgagtgggag ctgaccaaga
      481 gcccggccgg cgcaaagcag tgggtggcga agaacggcgc ggcgcccgtg cccgacgctc
      541 acgatccttc gaaacaccac aaggccggca tgctgacgac cgatctcgcg ctgcgctacg
      601 acccgatcta cgaaaagatc tcgcgacgct tctacgagca tcccgatcag tttgccgatg
      661 cgttcgcgcg cgcgtggttc aagctgacgc atcgcgacat ggggccgcgc gcacgatatc
      721 tcgggccgga agtgccggcg gaagagctca tttggcaaga tccggttccg aaggtcgatc
      781 atccgctggt cgacgcaacc gacgttgcgg cgctcaagca acggattctc gactccgggc
      841 tctcgatctc ggagctcgtc tcgaccgcct gggcgtcggc gtcgacgttc cgcgcgagcg
      901 acaagcgcgg cggggcgaac ggcgcgcgca ttcgcctcgc accgcaaaag gattgggagg
      961 tgaatcgtcc ggcacagctg gccgccgtgc tgaaaaagct cgaagagatc cggcactcgt
     1021 tcaacggcgc gcaaaacaac ggcaagaaga tctcgcttgc cgacctgatc gtactcgcgg
     1081 ggtgtgcggg cgtggagaag gccgcgaaga acgccggatt cgacgtcacg gtgccgttcg
     1141 cgccggggcg caccgacgca tcgcaagagc agaccgacgt ggagtcgttc gccgtgctcg
     1201 agccggttgc cgacggattc cgcaactacc tcaaagacgg cgtcgcgatt ccggcggaaa
     1261 tgatgctcgt cgacaaggcc cagctcatga cgctgaccgc gccggagatg accgtgcttc
     1321 tgggcggcat gcgcgtgctc gaaacgaacg ccgacggctc gaagcacggc gtcttcacct
     1381 cgaggccggg tgcgctcacg accgatttct tcgtgaatct gctcgacatg cacggtgagt
     1441 ggaaagagaa ggctgcaaat ctgtacgaac ggcaaaactc taaggcgggc accgagacgg
     1501 ggacgcgcgt cgatctgatc ttcggctcga actccgaact acgagcggtc gccgaaacct
     1561 acggaagttc ggacgcaagc cgaaagttcg tcgacgactt cgtcgcggcg tgggcgaagg
     1621 taatgaatct ggatcgctac gaccttgcag gatagttcgg ccgtaggtcc ggcgtgagct
     1681 cgcgcaccgc ggcggcggtc gtcgctttcg ccgccataat tctggccggg tgttccggcg
     1741 cgaccgggtc gcgcctcacc ccgggcggcg cggccatccc gctcggcgcg gcttccccga
     1801 cgacggccta cacgtattac gatctcggcc ccggaccgaa tggcaaagat gcgtacggct
     1861 acggtgagag caccacggca caggccggcg cgtacttcga tcacaccatt tcgtgcggcg
     1921 aggattgctc ggcgaaagtg tattacccgc tcgcgtggtc gggacagggc tccagcccgg
     1981 tcgtcctctt gccccaacat tacgtcgaag gttgggcgta cggtgccggc ggctcgctga
     2041 tcgtcgggac cctcatcacg aatgacggcg gctacttcgg atttgcccac gccgcgttgt
     2101 ggcagggttc ttcgcacacc ttcaccgacc tcaacccggc cggcaattgc ggaagctgct
     2161 cgccgggatc gaatgcgtac ggcaccgacg gaacgtttat cgccggatcc ggcggatcgc
     2221 cgcaacacgc gctcctctgg aagctcgcgc agctgaagac gccggtcgac ctgaatccgt
     2281 ccggctttgc gtattccgaa gcctacgcgg tgcgcggtac
//
LOCUS       DASUCJ010000381         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_263916, whole genome shotgun sequence.
ACCESSION   DASUCJ010000381 DASUCJ010000000
VERSION     DASUCJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263916"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFU90_04600"
     CDS             complement(<1..312)
                     /locus_tag="VFU90_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479087.1"
                     /translation="MAIHCRIIAVALAGTLAGALAVSGAFAQAPASPGAGIVKRGEYL
                     AIAGDCGACHTKPGGKPFAGGGVVPTPVGDVVSTNITPSKTAGIGNDTLGQFSEAVRR
                     GI"
     gene            complement(316..>1717)
                     /locus_tag="VFU90_04605"
     CDS             complement(316..>1717)
                     /locus_tag="VFU90_04605"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5479088.1"
                     /translation="NNYLMQTGPYPYAAEYIRQVGGTTWHWAAHAWRNVPNDFRIKSL
                     YDVGVDWPLSYDELEPFYQEAEEIMGVSGAPNTGSPRTKPFPMEPVAEPWAMRRIRER
                     LASAYPVVTNTTARNSRTYDGRPACCGNNSCQPICPVDAQYNGGVAAASAEAAGVKLL
                     PNANVYRLEHDANARISAALYYDPDKRSHRVTGKTFIVAANGIESPRLLLLSASDRFP
                     HGLANSSDMVGRNLMDHPSTSLTFDADEDMWLGRGPQSPSSINTLRDGAFRAEHAPYR
                     LDFTNISRVDGATKALLAQGVYGSEFAKRLRHSAARELNVKNVLEVLPNPEHRITLAD
                     EKDAMGIPKPQAHYSIDDYTRRGAERSKEDFRRIASLLGGTNLRFSADGDYANNQHIC
                     GTVSIGADPHTSVCDAFGRTHDHENLFCVGTGVIPTCGTCNSTENGVALALRSAVEML
                     SKAGARPAAPSAEAGK"
BASE COUNT          250 a          557 c          599 g          311 t
ORIGIN      
        1 gatgccccgc ctcaccgctt ccgagaattg gccgagcgtg tcgttgccga tacccgccgt
       61 cttcgacggt gtgatgttgg tcgagacgac gtcgccgacc ggcgtcggaa ccacgccgcc
      121 gcccgcgaag ggcttgcccc cgggcttcgt atggcacgct ccgcagtcgc cggcgatcgc
      181 gaggtattca ccgcgcttca caatgcccgc gccggggctc gccggtgcct gtgcgaatgc
      241 gcccgacacg gcgagcgcgc ccgcgagcgt gccggcgagt gcgaccgcga tgattcggca
      301 gtgaatggcc atcgtctact tgccggcctc ggccgacggc gccgccggcc gtgctccggc
      361 cttgctcaac atctcgacgg cgctgcgcaa ggcgagcgcc acgccgttct cggtcgaatt
      421 gcaggtaccg caggtgggaa tcacgccggt accgacgcag aataggttct cgtgatcgtg
      481 ggtgcgcccg aatgcgtcgc acaccgacgt atgggggtcg gcgccgatcg agaccgtgcc
      541 gcagatgtgc tggttgttcg cgtagtcacc atccgccgag aagcgcagat tggtgccccc
      601 gagcagcgag gcgatgcgcc ggaagtcttc cttcgagcgc tcggcccctc ttcgtgtgta
      661 gtcgtcgatc gagtagtgcg cctgcggctt cggaatgccc atcgcatcct tctcgtcggc
      721 gagcgtgatg cggtgttcgg ggttcgggag cacctcgagt acgttcttca cgttcaactc
      781 gcgcgcggcg ctgtgccgca gccgcttcgc gaattccgag ccgtagacac cttgcgcgag
      841 cagcgctttc gtcgcaccgt caacgcggga aatgttggtg aagtcgaggc ggtagggcgc
      901 gtgctcggca cggaacgcgc catcgcgcag cgtgttgatc gaacttgggc tctgcggacc
      961 gcgcccgagc cacatgtcct cgtcggcgtc gaaggtgagc gaggtgctcg gatggtccat
     1021 caggttgcgg cccaccatgt ccgagctgtt cgcgaggccg tgcggaaagc ggtcgctggc
     1081 ggacagcagc agcaggcgcg ggctctcgat gccgttggcg gcgacgatga aagtcttgcc
     1141 ggtgacacgg tgcgagcgct tgtcgggatc gtagtagagc gcggcgctga tgcgcgcatt
     1201 ggcatcgtgc tcgagccggt agacgttcgc gttgggcagc agcttcacac cggcggcctc
     1261 ggccgacgcc gcggcgacac cgccgttgta ctgcgcatcg accgggcaga tcggctggca
     1321 actgttgttg ccgcagcaag cgggccggcc gtcgtaggtt cggctgttgc gcgccgtcgt
     1381 gttcgtcacg accggatacg ccgacgcgag acgctcgcgg atgcggcgca tcgcccacgg
     1441 ctcggcgacc ggctccatcg ggaacggctt cgttcgcggc gacccggtat tcggcgcgcc
     1501 agagaccccc atgatctctt cggcctcctg gtagaacggt tcgagctcgt cgtagctgag
     1561 gggccaatcg accccaacgt cgtagagcga cttgatccgg aagtcgttcg gcacgttgcg
     1621 ccacgcgtgc gccgcccagt gccaggtggt gccgccgacc tgccggatgt attcggcggc
     1681 gtacggatac gggccggtct gcatcaggta gttattg
//
LOCUS       DASUCJ010000382         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_921135, whole genome shotgun sequence.
ACCESSION   DASUCJ010000382 DASUCJ010000000
VERSION     DASUCJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921135"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..>1116)
                     /locus_tag="VFU90_04610"
     CDS             complement(8..>1116)
                     /locus_tag="VFU90_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5479089.1"
                     /translation="ARDNARRNPQRTASTASALMIGLALVTLVSVMAAGITKSFRGAV
                     DDLWVSGYAITAQNNFDPIPIAAGNAAARTPGVQAIANVRGGDAQIFNGVHQATAVGP
                     GGAQIFNIKWLDGGSNSVFTRLGAHGAFVDNNFAKTHHLTIGSALTLTAPTGRSVDLH
                     VIGIFKPPTGGSPFGPVTISDTAWDKIYDTPLNLFSFVRMKGGESDAAKAALERQLKA
                     FPNAKVQTKKEFIDNQISGLNSILNVLFVLLALSVIVSLFGIVNTLVLTVFERTRELG
                     MLRAIGMTRRQVRRMIRAESVITALMGGVIGIVLGLVLGGLLAARIDFIVFALPWVQV
                     IIFGVAAVIVGLLAAIFPARRAGKLNVLEALQYE"
BASE COUNT          180 a          354 c          391 g          191 t
ORIGIN      
        1 ccactgccta ctcgtactgc agggcctcca ggacgttcag cttgccggct cggcgggccg
       61 ggaagatcgc cgcgagcagc ccgacgatga cggcggcgac tccgaagatg atcacctgca
      121 cccatggcag ggcgaacacg atgaagtcga tgcgcgcggc cagcagtccg ccgagcacga
      181 gcccgagcac gatcccgatc acgccgccca tcagtgcggt gatgacgctc tcggcgcgga
      241 tcatgcggcg cacctgccga cgcgtcatgc cgatcgcgcg gagcatgccg agctcccggg
      301 tgcgctcgaa cacggtgagc acgagcgtgt tgacgatgcc gaacaggctc acgatcacgg
      361 acagcgcgag gagcacgaac aggacgttga gaatcgagtt caggccgctg atctggttgt
      421 cgatgaactc tttcttcgtc tgcactttcg cgttcgggaa ggctttcagc tgccgctcga
      481 gcgcggcctt ggctgcgtcc gattcgccgc ccttcatccg gacgaaggaa aagaggttca
      541 gcggcgtgtc gtagatcttg tcccaggcgg tgtcggagat cgtgacgggg ccgaagggcg
      601 agccaccggt cggtggcttg aagatgccga tcacgtgcag gtcgacgctc cttccggtcg
      661 gtgcggtcag ggtcagcgcc gagccgatcg tcagatggtg cgtcttcgcg aagttgttgt
      721 cgacgaatgc gccgtgcgcg ccgaggcgcg tgaagaccga gttgctgccg ccgtccagcc
      781 acttgatgtt gaagatctgg gcgccgccgg gccctacggc cgtcgcctgg tgcacgccgt
      841 tgaagatctg cgcgtcgccg ccgcgaacgt tggcgatcgc ctggacgccg ggcgtgcggg
      901 cggccgcgtt tccggccgcg atcgggatcg ggtcgaagtt gttctgcgcc gtgatggcgt
      961 aaccgctgac ccagaggtcg tcgaccgctc cgcggaagct cttggtgatt cccgccgcca
     1021 tcacggagac gagcgtcacg agcgcgagcc cgatcatcaa cgccgaggcg gtcgaggctg
     1081 tccgctgcgg gttccgacgc gcgttgtcgc gggcga
//
LOCUS       DASUCJ010000383         1471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_508453, whole genome shotgun sequence.
ACCESSION   DASUCJ010000383 DASUCJ010000000
VERSION     DASUCJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508453"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFU90_04615"
     CDS             <1..666
                     /locus_tag="VFU90_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479090.1"
                     /translation="DLGEQFYLWEVAVAAAGVILGIDAFDQPNVQESKDNTVALLKEY
                     AQTGTFAEPRTDVENHAFALSYLSGSKNLPSQNPVQALAGLLAQLRPHDYNAITAYVA
                     RNPEHIGLLEELRVKIRDARKVATTVGFGPRFLHSTGQLHKGGPDTCVVLQIVADDTE
                     DPKIPGMGLGFRTLLAAQALGDWMSLDKRNRRGVRVHLKGDVAAGLRALISAVDEALS
                     VRA"
     gene            663..>1471
                     /locus_tag="VFU90_04620"
     CDS             663..>1471
                     /locus_tag="VFU90_04620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5479091.1"
                     /translation="MIAAAPTTDGSVNPLREGLPPSDRIDEPCSIVFFGASGDLFKRM
                     LLPAVYAMRCNGTLPTDVALVGFARTPYDDAGFREYCRQQLDEFMPPDRKPEGALWDD
                     FARRISYVTADFKGTQDFLKLKEVLARNDAQFGTGGNRLFYLATPPSVFPVVIQQLKK
                     AGLESHDGDGSWTRIVVEKPFGTDLDSARALQRELEKVFPESAVYRIDHYLGKEPVQD
                     IIALRFANTIFEPIWNRNYVSNVQITAAESLGVEERGGYYDHAGALRDMIQ"
BASE COUNT          274 a          516 c          447 g          234 t
ORIGIN      
        1 gacctcggcg agcagttcta tctgtgggaa gtcgccgtcg ccgccgccgg cgtgatcttg
       61 ggaatcgatg cgttcgatca gcccaacgtc caggagtcga aagacaacac cgtcgcgctg
      121 ctcaaggagt acgcgcagac cggcacgttc gccgagccga gaaccgacgt cgagaatcac
      181 gccttcgcgc tctcgtatct ctcgggcagc aagaatctcc cgtcgcaaaa cccggttcag
      241 gcgcttgccg gactgctcgc gcagctgcgc ccgcacgact acaacgcgat caccgcctac
      301 gtcgcgcgca accccgaaca catcggcttg ctcgaagagc tgcgcgtgaa gatccgcgac
      361 gcccgcaagg tcgccactac cgtcggcttc ggtccgcggt tcctgcactc gaccggacag
      421 ctgcacaaag gcggcccgga tacgtgcgtc gtcctgcaaa tcgtcgccga cgacaccgaa
      481 gatccgaaga tccccgggat ggggctcggc ttccgcacgc tgctcgcagc gcaagcgctg
      541 ggcgattgga tgtcgctcga taagcgcaac cgacgcggcg tgcgcgttca cctcaagggg
      601 gacgtcgccg ccgggctgcg cgcgctgatt tcggccgtcg acgaggcgct ctcggtccgg
      661 gcatgatcgc cgccgccccc accacggacg gctccgtcaa tccgctccgc gagggccttc
      721 cgccgagcga ccggatcgac gagccgtgca gcatcgtgtt cttcggcgcc agcggcgact
      781 tgttcaaacg catgctgctg cccgcggtct acgcgatgcg ctgcaacggc acgctgccca
      841 ccgacgtcgc gctggtgggc ttcgcgcgca cgccctacga cgacgccgga ttccgcgaat
      901 actgccggca gcagctcgac gagttcatgc cgccggatcg caaacccgag ggcgcgctct
      961 gggacgactt cgcgcgccgg atcagctacg tcaccgccga tttcaagggc acgcaagact
     1021 tcctcaaact caaagaggtg ctcgcgcgca acgatgccca gttcggaacg ggcggcaacc
     1081 ggctcttcta tctggccacc ccgccgtcgg tctttccggt cgtcatccag caactcaaga
     1141 aggcggggct cgaatcgcac gacggcgacg gctcgtggac gcgtatcgtc gtcgaaaaac
     1201 cgttcggaac ggatctcgat tcggcgcgcg cgctgcagcg cgaactcgag aaggtcttcc
     1261 cggaaagcgc cgtctaccgc atcgaccact atctcggcaa agagccggta caagacatca
     1321 tcgcgctgcg cttcgccaac acgatcttcg agccgatctg gaaccgcaac tacgtttcca
     1381 acgtgcagat tacggcggcc gagtcgctcg gcgtggaaga gcgcggcggc tactacgatc
     1441 acgccggcgc cctgcgcgac atgattcaaa a
//
LOCUS       DASUCJ010000384         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_997539, whole genome shotgun sequence.
ACCESSION   DASUCJ010000384 DASUCJ010000000
VERSION     DASUCJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997539"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /locus_tag="VFU90_04625"
     CDS             complement(<1..>1131)
                     /locus_tag="VFU90_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5479092.1"
                     /translation="YSGGLTGQGLYGTLGFSDNNLHGTGNSGSIQFEKGARTGVAQIS
                     LQIPYLGNSPQSQKYSVGGSIFTNSQTYYYPVYGVTSAPGIPSVGGTPAPIPVTLYPG
                     TNAQQVNGVVATSESSSTGLNATIGRRLSDYTILSLQAGAQTIRYNTTVPSPYYFQGN
                     QPNIFVGPTPGPINSSVNSYGGSFGISASSIANVNTGAPYKLNTAILSLQSNNLDDPF
                     NPRRGINGLFSETLSIPAFGSNFQFTQSSLSIAKFFPVLKTATLGVHLLLDGSTGVIP
                     PSSLFTFSDQQMRGYNQVFYATDAGLGQIELRQPLALDRRLTVAVFVDELDFRIRGAY
                     PLLNPYTNRIVGYPANWALYGDYGAGIRFDVPQLGLRTIRIDF"
BASE COUNT          210 a          318 c          393 g          210 t
ORIGIN      
        1 aaaatcgatg cgaatggtgc gcaacccgag ctgcggcacg tcgaatcgga ttcccgcgcc
       61 gtagtcgccg tagagcgccc agttcgccgg atagccgacg atgcggttgg tgtagggatt
      121 gagcagcggg tacgcgccgc ggattcggaa atcgagctcg tcgacgaaca ccgccaccgt
      181 caggcgccgg tccagcgcga gcggctgccg cagctcgatc tggccgagcc cggcgtcggt
      241 ggcgtaaaag acttggttgt agccgcgcat ctgctggtcg gagaacgtga acagcgagct
      301 cggcggtatc acgcccgtcg agccgtcgag cagcaagtga acgccgagcg tcgccgtctt
      361 gagcacgggg aagaattttg cgatgctcag cgacgactgc gtgaactgga agttcgagcc
      421 gaacgccggt atcgacagcg tctcggagaa cagtccgttg attcctcggc gcggattgaa
      481 cggatcgtcc aggttgttgc tctgcaggct gaggatcgcg gtattgagct tgtacggagc
      541 gccggtattg acgttggcga tcgaggaggc cgagatgccg aacgagccgc cgtacgagtt
      601 caccgaagaa ttgatcgggc cgggcgtcgg gccgacgaag atgttcggct gattgccttg
      661 gaagtaatac ggcgacggca ccgtcgtgtt gtaccgaatc gtttgcgcgc cggcctgtag
      721 ggaaaggatc gtatagtcgc tgagccggcg gccgatggtt gcgttgagac ccgtcgagct
      781 cgactcgctg gtagcgacga caccgttgac ctgctgcgcg ttggtcccgg gatacagcgt
      841 gaccggaatc ggcgccggcg tgccgcccac cgacggaatg cccggcgccg acgtcacgcc
      901 gtacaccgga tagtagtacg tctggctgtt ggtgaagatc gatccgccga cgctgtactt
      961 ctgcgactgc ggcgagttgc cgagatacgg aatctgcagc gagatttgag cgacgccggt
     1021 gcgcgcgccc ttctcgaact ggatcgatcc ggagtttccg gtgccgtgca ggttgttgtc
     1081 ggaaaatccg agcgttccgt agagaccttg acccgtgagg ccgccggaat a
//
LOCUS       DASUCJ010000385         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_997560, whole genome shotgun sequence.
ACCESSION   DASUCJ010000385 DASUCJ010000000
VERSION     DASUCJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997560"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFU90_04630"
     CDS             complement(<1..559)
                     /locus_tag="VFU90_04630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5479093.1"
                     /translation="MPDDARRPSFDKAKLPSRHTTVGPERAPHRSYLYAMGLTAEQIA
                     QPLIGVVSTWNEAAPCNIALGRQAQAAKRGVAEAGGTPREFTTITVTDGIAMGHAGMK
                     SSLVSREVIADSVELTMRGHCYDAMVGLAGCDKSLPGLMMAMLRLNVPAVFLYGGSIL
                     PGRFHGKDVTVVDVFEAVGKHSAGNM"
     gene            655..1077
                     /gene="hisI"
                     /locus_tag="VFU90_04635"
     CDS             655..1077
                     /gene="hisI"
                     /locus_tag="VFU90_04635"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEU5479094.1"
                     /translation="MMAAKERVMADALSALLDEAKFDAGGLIPAIAQQHDSGEVLTLA
                     YMNRDSLAATLQSGQVTYWSRERKKLWRKGETSGQTQRLVELRLDCDGDAILLLVDQN
                     GVACHTGRKSCFYRAIRDDTLVTIAEPLVSPDALYPKS"
BASE COUNT          191 a          367 c          354 g          191 t
ORIGIN      
        1 tcatgttccc ggcactatgc ttgccgaccg cttcgaacac gtcgaccaca gtgacgtcct
       61 tgccgtggaa ccggccgggc aggatcgagc cgccataaag gaacaccgcc ggcacgttga
      121 gccgcagcat cgccatcatg aggccgggca gcgacttgtc gcagcccgcg agcccgacca
      181 tggcgtcgta gcaatggccg cgcatcgtca attcgaccga atccgcgatc acttcgcgcg
      241 acaccagaga cgatttcatg ccggcatggc ccatcgcgat gccgtcggtg acggtgatcg
      301 tcgtgaactc gcgcggcgtg ccgcccgcct ccgcgacgcc gcgttttgcc gcttgcgcct
      361 ggcggccgag cgcgatgttg cacggggccg cctcgttcca ggtcgaaacc acgccgatca
      421 gcggctgcgc gatctgctcc gccgtcaggc ccatcgcata gagataggag cgatggggcg
      481 ctcgctccgg cccgaccgtg gtgtggcggc tgggcagctt cgctttgtcg aaactcgggc
      541 ggcgcgcgtc gtcgggcatg gcactctctc cagtcaaaag gtcgcgggag catagggaaa
      601 tcgcccgccg ccagcaataa tcgctgtggt ttgagcgggt gacgctatcc acttatgatg
      661 gcggcgaagg agcgtgtcat ggcggatgca ttgtcggcgt tgctagacga ggcgaaattc
      721 gacgcgggcg ggctgatccc ggcgatcgcg cagcagcacg attccggcga ggtgctgacc
      781 ctcgcctata tgaaccgcga ttcgcttgcc gccacgctgc aatcgggtca ggtcacctat
      841 tggtcgcgcg aacgcaagaa actgtggcgc aagggcgaga cgtcgggcca gacccagcgc
      901 ctcgtcgagc tgcggctcga ttgcgacggc gacgcgattc tccttctggt cgaccagaac
      961 ggcgtcgcct gtcacaccgg acggaaaagc tgcttctatc gtgcgatccg cgacgacacg
     1021 ctcgtcacca tcgccgaacc gctggtgtcg cccgacgcgc tctacccgaa gagctagctc
     1081 ctcgccagct cacgctcgag ctt
//
LOCUS       DASUCJ010000386         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1226942, whole genome shotgun sequence.
ACCESSION   DASUCJ010000386 DASUCJ010000000
VERSION     DASUCJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226942"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="fni"
                     /locus_tag="VFU90_04640"
     CDS             complement(<1..808)
                     /gene="fni"
                     /locus_tag="VFU90_04640"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU5479095.1"
                     /translation="MPREQPEPRTRARKAEHLRINVDEDVGGKGITNGFEAYAFVHNA
                     LPEIDLDEVDLSTRFLNRRLGAPLLISCMTGGVAEATRINRALARVAQEFGLAMGLGS
                     GRALLESPASLETFDVRKFAPDIVLLANLGAVQLNLGCDAGDCRRLMRLLNADALALH
                     LNPLQEALQPEGDTCFRGLLEKIALVCSQLGAPVIVKEVGWGIPPDTVRALFDAGVAA
                     VDLAGAGGTSWSEVERYRIGEPWRANVAAAFAGWGIPTAECLSGAVEIAPR"
     gene            complement(789..1565)
                     /locus_tag="VFU90_04645"
     CDS             complement(789..1565)
                     /locus_tag="VFU90_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5479096.1"
                     /translation="MPTASAPLPLGEHRARVLQYLRKIALPRRSVASNIIQELRPPDD
                     QRFLLRPGLVLWACSACDGDLGDALAVAAAFDLFDRFMLLHDELVEAPPDANPDSLVA
                     RWGLGQSLNAGDALYAMALRALAQDVGNPQRRLRAAALVTRAVLESIEGRTSDIEAGT
                     QGSAAGLLARVRSMRRRSAVLTGAALLAGAVIAGAPAATLQGFDRAGRLLDVAATSDD
                     RAIAKRLIDKAITVVEGCIPDPSQLGPFEEVARYVAARAA"
BASE COUNT          283 a          578 c          531 g          272 t
ORIGIN      
        1 cgcgcggcgc gatctccacc gcgccggaca aacattccgc ggtcgggatg ccccacccgg
       61 caaacgccgc cgcgacgttc gcgcgccacg gttcgccgat gcgataccgc tcgacttcgc
      121 tccatgacgt gccgccggca ccggccaagt cgaccgccgc gacgcccgcg tcgaagagcg
      181 cgcgcaccgt atccggcggg ataccccagc cgacttcctt cacaatcacc ggcgcgccga
      241 gctgcgagca caccagtgcg attttttcca gcagcccgcg aaaacaggtg tcgccctcgg
      301 gctgcagcgc ttcctgcaag ggattgagat gcagcgcaag ggcgtccgcg ttcaacagcc
      361 gcatcagccg ccggcagtcg ccggcgtcgc accccaggtt cagttggacg gctcccaggt
      421 tcgcaagcag gacgatgtcg ggcgcgaact ttcgcacgtc gaacgtctcc agcgaggccg
      481 gcgactccag caacgcacgg cccgatccga gacccatggc tagtccgaat tcttgagcca
      541 cgcgcgcgag cgcgcgattg attcgcgtcg cttccgcaac cccgccggtc atgcacgaaa
      601 tcaggagcgg tgcgccgagc cggcggttta aaaaacgggt cgacagatcg acctcgtcga
      661 ggtcgatctc gggcagagcg ttgtggacga acgcgtaggc ctcgaacccg ttggtgatgc
      721 cctttccgcc gacgtcctcg tcgacgttga tgcgaaggtg ctccgccttg cgcgctcgcg
      781 ttcggggctc aggctgctcg cgcggcaacg tatctcgcta cttcctcgaa gggtccaagt
      841 tggctcggat ccggaatgca gccttcgacg acggtgatgg ccttgtcgat cagccgcttg
      901 gcgatagccc gatcgtcgct ggttgcggcg acgtcgagca gccgacccgc gcgatcgaac
      961 ccttggagcg tcgccgccgg tgcaccagcg atgaccgcgc cggccagcag cgcggcgccg
     1021 gtaagtacgg cgctgcgccg gcgcatcgat cgcacccgcg cgagcaatcc cgctgccgat
     1081 ccctgcgttc ccgcttcgat gtcgctggtt ctcccttcga tcgactcgag caccgcgcgc
     1141 gtgacgagtg ccgccgctcg cagccggcgt tgtggatttc cgacgtcctg cgccagcgcg
     1201 cgcagcgcca tcgcgtacag cgcgtcgccg gcgttcaggc tttgaccgag cccccagcgt
     1261 gctaccagcg aatcgggatt tgcatccggc ggcgcttcca cgagctcgtc gtggagcagc
     1321 atgaatcggt cgaataaatc gaaggccgcc gccacggcaa gcgcgtcgcc caaatctccg
     1381 tcgcaagccg agcaagccca taggacgagc cccggccgaa gcaggaatcg ctgatcgtcc
     1441 ggtgggcgga gctcctggat gatattgctg gccaccgaac gtcgcggcag cgcgatttta
     1501 cgcaggtact gcaggacgcg agcgcgatgt tcgcccaagg gcagcggtgc ggaggccgtc
     1561 ggcatagcgg tcacgttcgc cggtccgtcc catcatccgc ccggagagta agcgatgcga
     1621 attggttccg aagaacacaa gcagctgttt tgcagatcgt tcgt
//
LOCUS       DASUCJ010000387         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_584999, whole genome shotgun sequence.
ACCESSION   DASUCJ010000387 DASUCJ010000000
VERSION     DASUCJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584999"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..3544
                     /locus_tag="VFU90_04650"
     CDS             218..3544
                     /locus_tag="VFU90_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479097.1"
                     /translation="MTKAFLRRPVLAIVCSLIVLIGGGVVIPGLAVAQYPQIAPPVVT
                     VSAVYVGASPQAVEAQVTTPLEQAVNTVQGLRYISSTSAQGVSTITLTFTLETNLDIA
                     AADVQNAAQTALGQLPASVQQLGVTVSKNSGTFVLGIALTSDTKNVDNLALSNYAQLN
                     IVNNLSRVAGVSGVRIFGQRQYAMRIWLNPRLLAQQGLDAGDVVSALQEQNVAVGVGS
                     IGAPPAPKNQAHTYTVNAVTQLSSPQEFANIILRANADGGFTRLGDVARVELGAQDYS
                     SALRFDGESRVVGLGVQQYSTANALDVSKAVLAKMEQLSQSFPAGVHYTVAFNTTTFV
                     RESIREVLITLLLSIVLVVLVIYVFLQNPRSTLIPAVTIPVSLVGTFFVMKIFGFSIN
                     TITLFGITLATGLVVDDAIVVIENIARHIEMNRGKKSPLESAAEAMTEIQSAVVASSL
                     VLLAVFIPVGFFPGATGQLYKQFALTIAAAITISLFQALTLAPVLSARLLGEEPESRF
                     LFFRWFNHGLKRFRDWYSRQLPKAFRYRWAVAGAFGIALLLTGLMFLRTPQAFIPDED
                     QGYFIVLLQAPEGTSLEAEQTIAKKAEKLILAQPEVQHLFDVGGFSFSGAAPNRGIMF
                     ALLTPWGDRKCSFMSALMLQPCSHNVASVLQRLNGAFYMQIPQAQIFGFNPPAINGIG
                     SVGGFQFELEDRGNVGLDKLMGLSYAMMGAAAQDPRLTGVFTQFRVNSPQLQVDVDRN
                     KAKSIGISLNDIFQTMEVQLGSLYVNNFTYLNRSWQVDVQADAPFRNDVASLQQIYVH
                     NGSAAPNIPTGTNPFATPPPGSATQQTTATGVVMTPLNALMSVKMQLGPPVISHYNLY
                     RNIELQGSAAPGRSSGEAIAAMEQIAQRVLPNGVSYEWTGLQLDQIAAGPLAVIIFAL
                     GIVFVFLVLSAQYESFIDPLIVLLAVPAALFGALGFINFRHFPVPFLFDPSLAQDAYA
                     QVGYVMLIGLASKSAILIVEFANQQLREGATVVQAALRAAQTRLRPILMTSIAFVVAV
                     TPLVFASGAGNAARHSLGTVVFGGMLVSTFLNLAITPVLYVIIKSLELRGKGPERNGS
                     TRARGTAVDGEPQTRLVTKV"
     gene            3677..>4729
                     /locus_tag="VFU90_04655"
     CDS             3677..>4729
                     /locus_tag="VFU90_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479098.1"
                     /translation="MLGKALVCVAAIALAIASAGLSLLPASADGVENVYEQSILPGAN
                     TAIKNAMDEANRKREAERRQASAPAGPSTSLLPPGTVGQIPPVSQQRIGPAIGFPTGF
                     GFSADVSSAYPFGDIGSYGKQWLMGGFDGSLSYAFSPTSRVVASMFELQHWPYGFNSG
                     QVPVYLAGFRNPVGCADLSGGNACGPGAGQNLNVATKDTFGVFMYEQLFLIKGMPGGH
                     VLPIVISPTYVARGGQIGESPDNNDVVPFAFNPPDGPVFTSVPTRTAQYKAVAVTLPF
                     LKTPKMFGTFTVAPTWLMHTAGVNSGNSMQLAQVLYLEYTPFANTKIWFEPQSARDYL
                     PTDPYPEHLIAYFLGIS"
BASE COUNT          818 a         1579 c         1463 g          869 t
ORIGIN      
        1 gggccaatat ctgcagccca gcaagaagca tctgccggtc gtcgagttcg tcacgccgca
       61 gacgttcgcg gccctcggga aactcgcgcg cggcaagggc ttccatcaag tcgtgtcgag
      121 cccgctgtcg cgcagctcgt atcacgcgga gcaagccttt ccggatcgct gaccgcccct
      181 gcaaccgggg ctcgcgtggg cgagtttgta ggctaacgtg accaaggcat ttctccggcg
      241 gcccgtcctc gcaatcgtgt gctcgctgat cgtcctgatc ggcggcgggg tcgtgattcc
      301 gggcctggcg gtggcacaat acccgcagat cgcaccgccg gtggtgaccg tcagcgccgt
      361 ttacgtcggt gccagcccgc aggcagtcga ggcgcaggtc acgacgccgc tggagcaggc
      421 ggtaaacacc gtccagggac tgcgctacat ctcgtcgacc agcgcgcagg gcgtttcgac
      481 catcaccctg acgttcacgc tcgagaccaa tctcgacatc gccgcggccg acgtgcagaa
      541 cgccgcgcag accgcgctcg ggcagctccc tgcgagcgtt cagcaactgg gcgtcaccgt
      601 ctcgaagaac tccggaacgt tcgtcctggg gatcgcgctg acgtcggaca ccaagaacgt
      661 cgacaacctg gcgctcagca actacgcgca gctgaacatc gtcaacaacc tttcgcgcgt
      721 cgccggcgtt tccggcgtcc gcatcttcgg gcagcgccaa tacgcgatgc gcatctggct
      781 caacccgcgg ttgctcgccc agcaggggct cgacgcgggc gacgtggtga gcgcgctgca
      841 ggagcaaaac gtcgcggtcg gcgtcgggag catcggcgcg ccgcccgcac ccaaaaacca
      901 agcgcacacg tacaccgtca acgcggtgac ccagctgtcg tcgccgcagg agttcgccaa
      961 catcatcctg cgcgccaacg ccgacggcgg cttcacccgg ctgggcgacg ttgcccgggt
     1021 cgagctgggc gcccaggatt actccagcgc gctgcggttc gacggcgagt cccgggtggt
     1081 cggcttaggc gttcaacaat actccaccgc taacgcgctc gacgtttcca aggccgtgct
     1141 cgcgaagatg gagcagcttt cccaatcgtt cccggcgggc gttcattaca cggtcgcgtt
     1201 caacacgacc accttcgtgc gcgagtcgat cagggaggtc ctgatcaccc tgctgctgtc
     1261 gatcgtcctg gtcgtcctgg tcatctacgt gttcctccag aacccgcgtt cgacgctgat
     1321 tccggccgtg acgattcccg tgtcgctggt cgggacgttc ttcgtcatga agatcttcgg
     1381 gttctcgatc aacacgatca cgctcttcgg gatcacgctc gcgaccgggc tggtcgtcga
     1441 cgacgcgatc gtcgtcatcg agaacatcgc gcgccacatc gagatgaacc gcggcaagaa
     1501 gagcccgctg gagagcgccg ccgaagcgat gaccgagatt caaagcgcgg tcgtcgcctc
     1561 gtcgctggtg ctgctcgccg tcttcatccc ggtcgggttc ttcccgggcg caaccggcca
     1621 gctgtacaaa cagttcgcgc tgacgatcgc ggccgcgata acgatctcgc tcttccaagc
     1681 gctgacgctc gcgccggtac tttcggcgcg cttgctcggc gaggagcccg aatcgcgctt
     1741 cctcttcttc cgctggttca accacggctt gaagcgcttc cgcgattggt attcgcgcca
     1801 gctgcccaag gcgtttcgct atcgctgggc ggtggcgggc gcgttcggca tcgcgctgct
     1861 gctgaccggc ttgatgttcc tgcgcacgcc gcaggccttc atccccgacg aggatcaggg
     1921 ttacttcatc gtgctgctgc aagcgccgga agggacgtcg ctggaagcgg aacagacgat
     1981 cgcgaaaaag gccgaaaaac tcatcctcgc gcagcccgaa gtgcagcatc tgttcgacgt
     2041 cggcggattc agcttcagcg gcgcggcccc gaatcgcggt atcatgttcg cgctgctcac
     2101 gccgtggggc gaccgcaagt gctcgttcat gagcgccctg atgctgcagc cgtgcagcca
     2161 caacgtcgct tcggtcctgc agcggctcaa cggcgcgttc tacatgcaaa ttccccaggc
     2221 gcagattttc ggcttcaacc cgccggcgat caacggcatc ggctcggtcg gcggcttcca
     2281 gttcgagctg gaagaccgcg gcaacgtcgg cctcgacaag ctgatggggc tcagctacgc
     2341 gatgatgggc gccgcggccc aagatccgcg gctgaccggc gtgttcacgc agtttcgcgt
     2401 caactccccg cagctgcagg tcgacgtcga ccgcaacaaa gccaagtcga tcggaatatc
     2461 gctcaacgac attttccaaa ccatggaagt acagctcggc tcgctgtacg tcaacaactt
     2521 cacctatctc aaccgctcgt ggcaggtcga cgtgcaggcc gacgcaccgt tccgcaacga
     2581 cgtcgcatcg ctgcagcaga tttacgtgca caacggctcc gccgcgccga acatcccgac
     2641 cggcaccaat ccgttcgcga ccccgccccc gggatcggcg acccagcaaa cgaccgcgac
     2701 cggcgtcgtg atgacgccgc tgaacgcgct gatgagcgtc aagatgcagc tcgggccgcc
     2761 ggtgatctcg cactacaacc tctaccgcaa catcgagctg cagggcagcg ccgcgccggg
     2821 ccgcagctcg ggcgaagcga tcgcggcaat ggagcagatc gcccagcgcg ttctgcccaa
     2881 cggcgtctcg tacgagtgga ccggcctgca gctcgatcag atcgcggccg gtccgctggc
     2941 ggtgatcatc ttcgcgctcg gaatcgtgtt cgtcttcctg gtgctgtcgg cgcagtacga
     3001 aagcttcatc gacccgctga tcgtgctgct ggccgtgccc gcggcgttgt tcggcgccct
     3061 cggcttcatc aatttccgcc atttcccggt accgttcttg ttcgatccga gcctggcaca
     3121 ggacgcctac gcgcaggtcg gttacgtcat gctgatcgga ctcgccagca agagcgcgat
     3181 tctgatcgtg gagttcgcca accagcagct gcgcgagggc gcgacggtcg tgcaggcggc
     3241 gctgcgggcg gcgcagacgc ggcttcgccc gattctgatg acgtcgattg cgttcgtggt
     3301 cgcggtgacc ccgctggtct tcgcatcggg tgcgggcaac gccgcgcggc attcgctcgg
     3361 aacggtggta ttcggcggaa tgctcgtatc gacgttcctg aacctcgcga tcacgccggt
     3421 gctgtacgtc atcatcaagt cgctcgaatt gcgcggcaaa gggcccgagc gcaacggcag
     3481 cacgcgagcg cgcggcacgg cggtcgacgg cgagccgcaa acgcgtttgg tgactaaggt
     3541 ttaggaacgc cgcgttctcg aagtagtttt tcggcgcaaa ccagggtttt aaagtttttt
     3601 agagcaacgt cccgcccgaa aggtgcccgc gcctagacga aaccgtcggg catcgccgcc
     3661 gattgggagt tatcgcatgc tcggaaaagc gctcgtttgc gtcgccgcaa tcgcacttgc
     3721 gatcgcgtct gccggtctgt ccctcctacc ggcaagcgcc gacggggtcg agaacgtcta
     3781 cgagcaaagc attctgcccg gcgccaatac tgcgatcaag aatgcgatgg acgaggcgaa
     3841 ccgcaaacgg gaagccgagc gccggcaagc ttccgcgccg gccggaccgt cgaccagttt
     3901 gcttccgccc gggacggtcg gtcagatccc tccggtgagc cagcagcgca tcggcccggc
     3961 catcggattt ccgaccggct tcggcttcag cgcggacgta tcgtccgcat atccgttcgg
     4021 cgacatcggc tcgtacggca aacagtggct gatgggcggt ttcgacggat ccctatcgta
     4081 cgcgttcagc ccaacctcgc gggtggtcgc gagcatgttc gagctgcagc actggccgta
     4141 cggtttcaac agcggtcaag tgccggtgta tctcgccggt ttcaggaatc cggtgggttg
     4201 cgccgatttg agcggcggga acgcgtgcgg gccgggggcc gggcagaatc tcaacgtcgc
     4261 caccaaagat acgttcggcg ttttcatgta cgagcagctg ttcctgatta aggggatgcc
     4321 gggcggtcac gtcttgccga tcgtaatctc gccgacgtac gtcgcgcgcg gcggccagat
     4381 cggcgagtcg cccgataaca acgacgtcgt tccgtttgcc ttcaatccgc ccgacggccc
     4441 ggtgtttacc agcgttccga cccgcaccgc gcaatacaaa gccgtcgccg tgacgctgcc
     4501 cttcctcaag acgccgaaga tgttcggcac gttcacggtt gcgccgacct ggctgatgca
     4561 taccgccggc gtcaactccg gaaactcgat gcagctcgcg caggtgctct acctcgagta
     4621 cacgccgttt gccaacacga agatttggtt cgagccgcag agcgcccgcg actatctgcc
     4681 gaccgatccg tatccggagc acctcattgc ctatttcttg ggcatctcg
//
LOCUS       DASUCJ010000388         1343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_799101, whole genome shotgun sequence.
ACCESSION   DASUCJ010000388 DASUCJ010000000
VERSION     DASUCJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799101"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1012)
                     /locus_tag="VFU90_04660"
     CDS             complement(233..1012)
                     /locus_tag="VFU90_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU5479099.1"
                     /translation="MKTTLLASAVLLALPLAALAADPPPPPTGWSGSGEAGLSIASGN
                     TKSQNLNAKLDIKFNDDQWTDDFYLLALRNKANTPAGYDLTANRYEGGASLGYKFDER
                     SYIVGAARYEHDEFSSYDYQYIVSIGYGYQILKNASDELAVEVGPGYKVAQPASYILL
                     PVDPQSPPVKVTPDSENNLVARGKLAYKHNFSDTVSFVDTFVVEAGSGNKFLQNDAGL
                     AVKMNSKLALKVGYQIRHNSDVEPGFKKTDGLFTTNLVYNF"
     gene            complement(1123..>1343)
                     /locus_tag="VFU90_04665"
     CDS             complement(1123..>1343)
                     /locus_tag="VFU90_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479100.1"
                     /translation="AARLRTICIVLIGAVPILAAVALFVLPGWSAIFACIAALAATAG
                     LFVERWLFFAEAKHVVMLYYGGVRPQSA"
BASE COUNT          248 a          385 c          460 g          250 t
ORIGIN      
        1 tgggcgaacg cgtcgcgcgc aatctgcttg cgcagggtgc cggggaattg ctggggcggt
       61 aattacgccc tcccgccggc gggaggggtg gagaattccg tgcaacggct gaaaaaccgt
      121 cgcgagcgat gacaggttgc acgccgccgg agcgcaggaa ccggagttta ctcggatgta
      181 aatgaggatt ccgagcaccg ccggcgtgca agatgtcgag cgcagcaggt ttttagaagt
      241 tgtagacgag gttcgtggtg aacaagccat ccgtcttctt gaaacccggc tccacgtccg
      301 agttgtggcg gatctggtag ccgaccttga gcgccagctt gctgttcatc ttcaccgcaa
      361 gtccggcgtc gttctggagg aacttgttgc cgctgccggc ttcgaccacg aacgtgtcga
      421 cgaacgacac cgtgtcgctg aaattgtgct tgtaggcgag cttgccgcgc gcaacgaggt
      481 tgttttccga atccggcgtg accttgaccg gcggcgattg cggatcgacc ggcaacagga
      541 tgtaggaagc cggctgcgcg accttgtagc ccgggccgac ttccaccgcg agttcgtcgc
      601 tcgcgttctt caggatctgg tagccgtagc cgatcgaaac gatgtactga tagtcgtacg
      661 acgagaattc gtcgtgctcg tagcgcgccg cgccgacgat gtagctgcgc tcgtcgaact
      721 tgtagccgag cgaggccccg ccttcgtagc ggttcgcggt caggtcgtag ccggccggcg
      781 tattggcctt gttgcgcagc gcgagcaggt agaaatcgtc cgtccactgg tcgtcgttga
      841 acttgatgtc gagcttggcg ttgaggttct gcgacttggt gtttccgctg gcgatggaca
      901 ggccggcttc gccgctgccg gaccagcccg tcggcggcgg cggcggatcg gcggcgagcg
      961 cggccagcgg cagcgcgagc aaaacggcgg atgcgagtaa ggtcgttttc atcgggaatt
     1021 ccttgttctg ggggacgcgc cgcgtgcgag gcggcgggcg cgcattgtaa acgcgcgccg
     1081 acgcaaaaaa gtggacttgt gcgcaaaacg tacaagcgcc gttcaggcac tttgcgggcg
     1141 cacaccgccg taatacaaca tcacgacgtg ctttgcttcg gcgaaaaaca gccagcgctc
     1201 gacgaacagg ccggccgtgg ccgcgagtgc cgcgatacac gcgaagatcg cgctccaacc
     1261 tggcagcacg aacagggcga ctgcagcgag aatgggcacg gcgccgatca ggacgataca
     1321 aatcgtgcgc aggcgcgcgg cgt
//
LOCUS       DASUCJ010000389         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_4346, whole genome shotgun sequence.
ACCESSION   DASUCJ010000389 DASUCJ010000000
VERSION     DASUCJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4346"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="VFU90_04670"
     CDS             complement(<1..2008)
                     /locus_tag="VFU90_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479101.1"
                     /translation="MDDPERERLDDMAMHPLSPWRRFGPYLSERQWGTVREDYSRDGT
                     AWEYLPHDHARSRAYRWGEDGIAGISDIHQHVCFALALWNGRDPILKERLFGLTGNEG
                     NHGEDVKEYYFYLDNVPSHAYMKMLYKYPQRAYPYAELVEENRRRTREDPEYELIDTG
                     IFDGDEYFDVTVEYAKIDPNDVLVRIGVTNRGPKPAELCVLPTLWFRNDWSWKPGVER
                     PELKLRKRYEWGSAVDVRHVKLEPFSFYTEGEDELLFTENETNNERLFGAPNAAPWVK
                     DGINDYVVRGKAEAVNQAGFGTKTSALHKLTVNPGETQYVRARLSSTTLETPFAAGFD
                     DAFERRRAEADAFYRNVNPHPITDELRAIQRQAFAGMLWTKQCYQFVVAEWLAGDPLT
                     PPPPPERLRGRNHDWGHLYAVDVISMPDKWEYPWFAAWDLAFHVVALALIDPEFAKHQ
                     LVLLTREWYMHPNGQLPAYEWNFGDVNPPVHAWAAGRVYQIEKKLRGKGDLEFLERVF
                     QKLLLNFTWWVNRKDVEGNNVFQGGFLGLDNIGIFDRSARLPTGGHINQSDGTSWMAV
                     YTLNLLAISLELAQHNHVYEDIASKFFEHFLYIAHALNNLAGDEGLWDEEDGFYYDVL
                     KLDDGTAIPIKVRSLVGLLPLLAVAVGERGMALRIPGFRERMIWF"
     gene            complement(2013..>2229)
                     /locus_tag="VFU90_04675"
     CDS             complement(2013..>2229)
                     /locus_tag="VFU90_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479102.1"
                     /translation="ECFAPLHGKRAAALARHAERLQTVLGERHDAVMIAQRLQSFGIA
                     QDVVVAPKPARWRPVWRRMRADYRRLR"
BASE COUNT          367 a          741 c          689 g          432 t
ORIGIN      
        1 gaaaccaaat catccgctcg cggaaccccg ggatccgcag cgccatcccg cgttcgccga
       61 ccgccaccgc cagcagcggc agcaggccca ccagcgagcg taccttgatc gggatcgccg
      121 ttccgtcgtc gagcttgagc acgtcgtaat agaagccgtc ctcttcgtcc cacagcccct
      181 cgtcgccggc cagattgttg agcgcgtgcg cgatgtagag gaagtgctcg aagaacttcg
      241 aggcgatgtc ttcgtacacg tgattgtgct gcgccagctc gagcgagatc gcgagcaggt
      301 tgagcgtgta gaccgccatc cagctggtgc cgtccgactg gttgatgtgg ccgccggtcg
      361 gcagacgcgc gctgcgatcg aagatgccga tgttgtcgag cccgagaaaa ccgccttgga
      421 agacgttgtt cccctcgacg tctttgcggt tgacccacca cgtgaaattc agcagcagct
      481 tttgaaagac gcgctccagg aattccaagt cgcccttgcc ccgcaatttc ttctcgattt
      541 gatacacgcg cccggccgcc caggcgtgca ccggcgggtt gacgtcgccg aagttccatt
      601 cgtaagccgg tagctggccg ttgggatgca tgtaccattc gcgcgtcagc aggaccagct
      661 gatgtttggc gaattcggga tcgatcagcg cgagcgcgac cacgtggaag gcgagatccc
      721 aggcagcgaa ccacgggtac tcccacttgt cgggcatcga aattacgtcg acggcataga
      781 ggtgccccca gtcgtggttg cggccgcgca gccgctccgg cggcggcggc ggggtgagcg
      841 ggtcgccggc cagccattcg gccaccacga actgatagca ctgcttcgtc cacagcatgc
      901 cggcgaacgc ctggcgctgg atcgcccgca gctcgtcggt gatcggatgc gggttgacgt
      961 tgcggtagaa cgcgtcggct tcggcgcggc gccgttcgaa cgcgtcgtcg aatcccgccg
     1021 cgaacggcgt ctcgagcgtc gtgctcgaca accgtgcccg cacgtattgc gtttcgcccg
     1081 gatttacggt cagcttgtgc agcgccgacg ttttcgttcc gaaaccggcc tggtttaccg
     1141 cttcggcctt ccccctgacg acgtagtcgt tgattccgtc ctttacccac ggcgcggcgt
     1201 tgggcgctcc gaacagccgc tcgttgttgg tctcgttttc ggtaaacagc agctcgtctt
     1261 cgccttcggt ataaaacgaa aacggttcga gcttgacgtg gcgaacgtcg accgcgctcc
     1321 cccactcgta gcgcttgcgc agtttgagct cgggccgctc gacccccggc ttccacgacc
     1381 agtcgttgcg aaaccacagc gtcggcagca cgcacagctc cgccggtttc ggcccgcgat
     1441 tggtgacgcc gatccgcacg agcacgtcgt tgggatcgat cttcgcgtat tcgaccgtca
     1501 cgtcgaagta ctcgtcgccg tcgaatatcc cggtatcgat cagctcgtac tccgggtcct
     1561 cgcgcgtacg ccggcgattc tcttcgacca gctccgcgta cggatacgcc cgctgcggat
     1621 acttgtagag catcttcatg taggcgtgcg acggcacgtt gtcgaggtaa aaatagtact
     1681 ccttgacgtc ctcgccgtgg ttgccttcgt ttccggtcag tccgaacaag cgctccttga
     1741 gaatcgggtc ccggccgttc cacagcgcga gcgcgaaaca cacgtgctgg tgaatgtccg
     1801 agatgccggc gattccgtct tcaccccagc ggtaggcgcg cgagcgcgcg tggtcgtgcg
     1861 gaaggtactc ccacgccgtt ccgtcgcggc tgtaatcctc gcggacggtg ccccactgcc
     1921 gctcgctcaa atacgggccg aagcgccgcc acggcgagag cgggtgcatc gccatgtcgt
     1981 ccaaacgttc gcgttccggg tcgtccatcg cgttaccgca gcctccggta gtcggcgcgc
     2041 atccggcgcc acaccgggcg ccagcgagcc ggcttgggcg caactactac gtcttgtgca
     2101 atcccgaaac tctgcaggcg ttgcgcgatc atcacggcgt cgtggcgctc gcccagcacc
     2161 gtttgcaggc gctcggcatg acgcgccagc gcggcggcgc gctttccgtg cagcggcgcg
     2221 aagcactcg
//
LOCUS       DASUCJ010000390         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_753004, whole genome shotgun sequence.
ACCESSION   DASUCJ010000390 DASUCJ010000000
VERSION     DASUCJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753004"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFU90_04680"
     CDS             <1..409
                     /locus_tag="VFU90_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479103.1"
                     /translation="AVPLRFFPRYVPCAVAITASMLEHFREAVGEPVRIAANGGYRSP
                     RHQLTTHASTHCWASAVNIYRIGDEYLDGRDRIERYAEIAREVLPGAWIRPVGEQLGY
                     ADDHLHIDFGYLIAVPHDAPGDPFNPKLEGDPL"
     gene            429..>1134
                     /locus_tag="VFU90_04685"
     CDS             429..>1134
                     /locus_tag="VFU90_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479104.1"
                     /translation="MAAPKERHKRERRVRLTTTGTRRRLPTAPIVVPPSLPAIGLEAE
                     FAVVLDGQPVRPEDVFGSPRRIVRGPLVHRTGRSYHLPTGSAVYFDTGVIEVATPMIE
                     LERGCAARAGRALWESIRFLRRELDVWQEDDGRDVNLVGFSTHYNVSFDLHADDQSNG
                     RTVDRLALLLTYVLPAPLMLLAANRQSTGIGVRPRGNRVEVTADFTPDAALMIAAATV
                     IVGIVRDVMSWPTFDLS"
BASE COUNT          178 a          398 c          363 g          195 t
ORIGIN      
        1 ggcggtaccg ctccggttct tcccccggta tgtgccctgc gccgtcgcaa tcaccgcttc
       61 gatgctcgag cactttcgcg aggcggttgg cgagcccgtt cgcatcgcgg cgaacggcgg
      121 atatcgctcg ccccgtcacc agctcacgac gcatgcatcc acgcattgct gggcgtcggc
      181 ggtgaacatc tatcgtatcg gcgatgagta tctcgacggc cgcgatcgca tcgagcggta
      241 cgcggagatc gcgcgcgagg tactgcccgg tgcctggatc cggccggtcg gcgagcagct
      301 cggctacgcc gacgatcacc tgcatatcga cttcggctac ctgatcgcgg tgcctcacga
      361 tgcaccgggt gatccgttca atccgaagtt ggagggcgac ccgctctgag cgggccggtg
      421 ttgcgcgcat ggcagcccca aaggaacgac acaaacgtga gcggcgcgtc cgcctaacga
      481 ccaccggcac gcgccggcgg ctcccgaccg cgccaatcgt cgttccgcca tccctgcccg
      541 caatcggact ggaggcggag ttcgccgtcg tgctcgacgg ccagccggtt cggccggagg
      601 acgtcttcgg ctccccgcgt cgcatcgtgc gagggccgct ggtacaccgc acgggacgat
      661 cgtatcacct gcccaccgga agcgcggtct acttcgacac gggcgtcatc gaggtcgcga
      721 cgccgatgat cgagctggaa cgcgggtgcg ctgcccgcgc tgggcgcgcg ctgtgggaga
      781 gcatacggtt cctgcgccgc gagctcgacg tctggcagga agacgacggt cgggacgtga
      841 acctcgtcgg cttcagcacg cactacaacg tttcgttcga cctgcatgcc gacgaccaat
      901 ccaacggacg cacggtcgat cggctcgcac tcctgctcac atacgtgctg ccggcgccgc
      961 tgatgctcct ggccgccaat cggcaatcca ccggtattgg cgtgcgaccg cgtggaaatc
     1021 gcgtcgaggt caccgcggac ttcacccccg acgccgctct catgatcgcc gcggccactg
     1081 tcatcgtcgg catcgttcgc gacgtcatga gctggccgac cttcgatctc tcgg
//
LOCUS       DASUCJ010000391         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_462898, whole genome shotgun sequence.
ACCESSION   DASUCJ010000391 DASUCJ010000000
VERSION     DASUCJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462898"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..>2629)
                     /locus_tag="VFU90_04690"
     CDS             complement(104..>2629)
                     /locus_tag="VFU90_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU5479105.1"
                     /translation="RDGRVRVATTGLRFDAVSRPGDVLADVSAPHADLEDFNNYFDTG
                     DTLAGRGSLAFSAAMTGDRLTTAGNIDVNGFRYRNLPIGDTRAQWSSARNVVAGSLEV
                     GGSEGMLHAGGSIGIAKAPDLESTFVRSRYDLRAIVDNLDLSLWVAALGFPNVPVTGR
                     ASGNATLHGRFPLVDLRGSAAIADGTIGPLTLETAKLSVHSIGSRIAVDGAELVTPGL
                     SATATGTMGVRPLDPLNLQVHAETDNLPRLIYQLARVKIPVSGAFESTLQIGGTYKAP
                     TFVAGLDATGVQAYGIGIASMFGEVRLHGRTLVLSNAGATFAKGSATLAGSLPLEISP
                     FRIGPPGQPVSFDVDVIGVDPAIFDAVLGNNTKLGGTIDGHFGLAGTLRQPVIVGHAS
                     LEKGSYVSDFERTPIGAVSARLTFDRTSASIDRFAASLGGGTFQGSGRVQFPQGFEST
                     AGYSFVAKGVAKGAQLNFPAYGNGTLDADLALTKTPASQAVLAGTVTLSNATLPFSAF
                     VQAATNAAGGLGFTLPPVAFDLTAAAGRAVRVRGSGYGAGLDIGVAGKVHLQGTFAAP
                     QLDGTITSTGGTLTYFDRAFRVQEGSVSFDPSDGVVPTIHAVATSSVVNPDPDRARNP
                     YGSAEITIDVDGPIQGLKIGFSSNPPGYTRDQIISMIAPFGGFINGIAFNGQSPYQVQ
                     SPSGITPLGTLSPLPEGAYQTRNGAITVGQEAFNILNAQFTAGLLAPLETALGQGLGL
                     SSVNLTLGYYGNVGVTATRLLGKTVSAVYATTFGLPQIQSFGIKFDPSAYTAATLSFY
                     YQTGPTKLFEQPVSVIGSSNQLLLAQPLLGNSGFSFSLQRYLY"
BASE COUNT          457 a          856 c          886 g          430 t
ORIGIN      
        1 cgcctcgccc gacgcggctc cttgccccca agattagcct aggattgagc ttagaacgca
       61 gcagccgagc gctggggcgg gtcgctatca cggggcggga acctcagtag aggtagcgct
      121 gcaaggaaaa gctgaagccg ctattgccca gcagcggttg ggccagcagc agctggttgg
      181 aggagccgat tacgctgacg ggctgctcga atagcttcgt cggcccggtc tgatagtaga
      241 agcttagcgt cgccgcggtg taggcgctcg ggtcgaactt gatgccgaac gactggatct
      301 gcggcagacc gaaggtcgtc gcgtagaccg cgctgaccgt cttgccgagc aaacgcgtcg
      361 cggtaacccc cacgttgccg taatagccca gcgtgaggtt gacgctcgat agtccgaggc
      421 cttggcccag ggccgtctcg agcggcgcga gcaagccggc cgtgaactgg gcgttgagga
      481 tgttgaacgc ttcctgtccc acggtgatcg cgccgttgcg agtttggtac gcgccctccg
      541 gcagcggcga gagcgtgccc agcggcgtga tgccggaggg cgactgcacc tgatagggcg
      601 attggccgtt gaacgcgatc ccgttgatga accctccgaa cggcgcgatc attgaaatga
      661 tttgatcccg cgtatagccg ggaggattgc tgctgaaacc gatcttcagc ccctgaatcg
      721 gaccgtcgac gtcgatcgtg atctctgccg agccgtacgg attgcgggca cggtcgggat
      781 ccggattgac gacgctcgag gtcgcgaccg catgaatcgt cggcacgaca ccgtcgctgg
      841 ggtcgaacga caccgagccc tcctgcaccc ggaacgcacg gtcgaagtac gtcagcgtcc
      901 cgccggtcga cgtaatcgtt ccgtcgagct gcggcgcggc gaaggtgcct tgcaagtgca
      961 ccttgccggc gacgccgatg tcgaggccgg cgccgtaacc gctcccccgg acgcgcacgg
     1021 cgcgaccggc ggcggcggtc aggtcgaacg ccaccggcgg aagcgtgaaa cccaggccgc
     1081 ccgcggcgtt cgtcgccgcc tgcacgaacg ccgaaaacgg cagcgtggcg ttgctcagcg
     1141 ttaccgtacc ggcgagtacg gcttgcgagg ccggcgtctt ggtcagcgcg aggtcggcgt
     1201 cgagcgtccc gttgccgtag gccggaaagt tcagctgcgc gcctttagcc acgcccttgg
     1261 caacgaacga ataacccgcc gtcgattcga agccttgcgg gaattgcacg cgcccggaac
     1321 cttggaacgt cccgccgccg agcgacgcgg caaaccggtc gatcgacgcg ctggtgcgat
     1381 cgaaggttaa gcgcgcgctg acggcgccga tcggcgtgcg ctcgaagtcg ctcacgtacg
     1441 aacctttttc caacgatgcg tgtccgacga tgacgggctg ccggagcgtg ccggcaaggc
     1501 cgaagtgccc gtcgatcgtt ccgcctagct tggtattgtt cccaagtacc gcgtcgaaga
     1561 tcgccgggtc gacgccgatc acgtcgacgt cgaagctcac cggctggccg ggcggtccga
     1621 tgcggaacgg cgagatctcc agcggcagcg atccggcgag ggtcgcactc cccttcgcaa
     1681 acgtcgcgcc ggcattcgac agcacgagcg tacgtccgtg cagacgaacc tcgccgaaca
     1741 tcgatgcgat cccgattccg tacgcctgca ctccggtggc gtccagtccg gcgacgaacg
     1801 tcggcgcctt gtacgtaccg ccgatttgca gcgtcgattc gaacgcgccc gaaaccggaa
     1861 tcttgacgcg cgcgagctgg taaataagcc gcgggagatt gtcggtttcc gcatggacct
     1921 gcaggtttag cggatcgagc ggacgcacgc ccatcgtccc cgtcgccgtc gcgctcaggc
     1981 cgggcgtcac cagttcggca ccgtcgacgg cgatccgcga gccgatcgaa tggacgctca
     2041 atttggcggt ttcgagggtg agcggaccga tcgtaccgtc ggcaatcgcg gcgctgccgc
     2101 gcaaatcgac cagcgggaag cgcccgtgca gcgtcgcgtt ccccgatgcc cgtccggtga
     2161 ccggaacgtt cgggaaaccg agcgcggcga cccaaagcga aagatcgaga ttgtcgacga
     2221 tcgcgcgcag atcgtagcgc gagcgcacga acgtcgactc gagatccggc gcctttgcga
     2281 taccgatcga tccgccggcg tgcagcatgc cttcgcttcc gccgacttcc agcgagcccg
     2341 ccacgacgtt gcgggcgctc gaccactgcg cgcgcgtgtc gccgatcggc agattacggt
     2401 accggaatcc gtttacgtcg atgtttcccg cggtggtcag gcggtcgccg gtcatcgccg
     2461 cggagaaggc gagcgatccg cggcccgcca gcgtatcgcc ggtgtcgaag tagttgttga
     2521 aatcctcgag atccgcgtgc ggcgcggaca cgtcggcgag tacgtcgccc ggacgcgaca
     2581 ccgcgtcgaa ccgcagtccg gtcgtcgcga cgcgaacgcg gccgtcgcg
//
LOCUS       DASUCJ010000392         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1318520, whole genome shotgun sequence.
ACCESSION   DASUCJ010000392 DASUCJ010000000
VERSION     DASUCJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318520"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFU90_04695"
     CDS             complement(<1..310)
                     /locus_tag="VFU90_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEU5479106.1"
                     /translation="MTGRGRISLSQVVAVAALTGSFLGASFVVHPSSALASADAAVAS
                     GVKPDAAHTVTYESNGTDAERTTSAATVGDFLHEQNVTVGSGDYVDPAIDVPISDGML
                     I"
     gene            742..>1532
                     /locus_tag="VFU90_04700"
     CDS             742..>1532
                     /locus_tag="VFU90_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479107.1"
                     /translation="MEHLRIGFFTEVYRPVVNGVVASIDALAEGLRGRGHDVYCFAPR
                     MPGGDENDGPVFRMPSLPLPTRTAYRLTLPLVSRRNVNNVIKRLSLVHVHSPFVTGWM
                     GMRYARRYGMPLVYTYHTQLEAYAHYVPFEPNATRYAASQLTRTFANLTDAVIVPTPA
                     MATRLRDLGVTARLEVVPSGIDVHRFGAGRRDEALRARLGAQPSDRLILCVSRLAKEK
                     NVELLLEAIAVARDPSLLLAIAGDGPAREELEARARECGVAARTR"
BASE COUNT          254 a          516 c          523 g          239 t
ORIGIN      
        1 ttatcagcat tccgtcggaa atcggaacgt cgatggccgg atcgacgtaa tcgccgctgc
       61 cgaccgtgac gttttgctcg tgcagaaaat cgccgaccgt tgcggcgctg gtggtccgtt
      121 ccgcatcggt gccgttggat tcataggtga cggtatgcgc cgcgtccggc ttcacgcccg
      181 aagcgacggc cgcgtcagcc gacgcgagcg ccgacgacgg gtgcaccacg aaactcgcac
      241 cgaggaagct gcccgtcagg gcggcgaccg cgacgacttg actgagactg atccggccgc
      301 gcccggtcaa acgaagggat tcctttctct ctcggctcga atcgcggtcg atccgcacaa
      361 acccgtggcg gacgaacggc aacgccgagc cgaatctcgc acatccggtg cgaaggcggg
      421 atatccggcg cggggagcac cggagggatg acgatcctcg ttacgttgtc ccgaaagggc
      481 ttgagaacgc catgcggagc gccggctgcc gaggctccgg ggagcccggg cacgtccgac
      541 gttcctgcga accgggctaa cgcacgcggt ccgcgggacg gctgccgaac cggcaaccgg
      601 gtctaacccg ggcactatag cacgcgcccg gaaagcagga cctatgccga aagtcgcacg
      661 cttgaaggag ctccctttaa gcggcgcagg cgaccgtttg gcgctagcgg accgcggcgg
      721 gtaaacgcga acgagcctgc cgtggagcat ctccgcatcg ggtttttcac cgaggtttac
      781 cggccggtcg tgaacggcgt cgtcgcgtcg atcgacgccc tcgccgaggg cttgcgagga
      841 cgcggtcacg acgtctactg ctttgcgccg cggatgccgg gcggcgacga aaacgacggc
      901 ccggtcttcc ggatgccgtc gctgccgtta ccgacgcgca ccgcctaccg gctgacgctt
      961 ccgctggtca gccggcgcaa cgtcaacaac gtcatcaaac gcctctcgct ggtgcacgtg
     1021 cactcgccgt tcgtcaccgg ctggatgggc atgcgttacg cgcgccggta cggcatgccg
     1081 ctggtataca cgtatcacac ccagctcgag gcatacgcgc actacgtccc gttcgaaccg
     1141 aacgctaccc gctacgcggc ttcgcagctg acgcggacct tcgcgaactt gaccgacgcg
     1201 gtgatcgttc cgacgcccgc gatggcgacg cggctgcggg atctcggcgt gaccgcgcgg
     1261 ctcgaagtcg tgccgagcgg gatcgacgtc caccggttcg gcgccggccg ccgcgacgaa
     1321 gctttgcgcg cgcgactcgg cgcgcagccg tcggaccggt tgatcctgtg cgtctcgcgg
     1381 ctggcgaagg aaaaaaacgt cgagctgctg ctggaggcga tcgcggtcgc gcgggatccg
     1441 tcgctgctgc tcgcaatcgc cggcgatggg ccggcccgcg aagagctgga ggcgcgggcc
     1501 cgcgagtgcg gcgtggccgc ccgcacgcgc tt
//
LOCUS       DASUCJ010000393         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_585120, whole genome shotgun sequence.
ACCESSION   DASUCJ010000393 DASUCJ010000000
VERSION     DASUCJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585120"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..620
                     /locus_tag="VFU90_04705"
     CDS             105..620
                     /locus_tag="VFU90_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479108.1"
                     /translation="MIAAIVLTALTLAQASPSETVPMTPLPATAPLESGACLDSSRPV
                     PRVLQPPVQRSNQVVRIDKVESTATLLNGEVIGFLYTLADGSTWLGQRTQPYMSGAAA
                     AAVNQVLAFTHMPGENVSKFPPQMAYGVATKYTQFFRVQIAASAPATLQIQVVPCVVW
                     PAGRPLPDPIM"
     gene            682..1062
                     /locus_tag="VFU90_04710"
     CDS             682..1062
                     /locus_tag="VFU90_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5479109.1"
                     /translation="MLRTRIGAFEPNADVFVDEEGGRVVAVVEIAGADPDTLHVGVDA
                     RDLYIGGQRLEAVRLRRGSFVQKEIAYGEFVKRIPLPVAVEFDGVAASYADGLLVVAL
                     PIAATAWRPTARTELHILVKRTHP"
     gene            1067..>2724
                     /gene="lon"
                     /locus_tag="VFU90_04715"
     CDS             1067..>2724
                     /gene="lon"
                     /locus_tag="VFU90_04715"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5479110.1"
                     /translation="MPEKKSTIVPANLIGILPLQEAVLFPHTVIPLAVVKKPGIQLVE
                     EALREGKPIGLSVLKDREIENPGPDDVHRVGTIGIIQKMLKVPDGTLRCIVAGQQVFK
                     VEQFTQVAPYMVAAYTELPDVTVENEQLVAMQRNLGGLFQKLLSYLPQAPREMEMEVQ
                     NITDPAVLTYFVASTMRLETADRQALLEERDTAKRMRKLTLLLTKELEVVELGHKIQS
                     DIQREMEKNQREFYLRQQLRAIQEELGEVDPQLAELNELREKVDAAGMPEDVKKAADR
                     ELDRLSKVPQASPEYSVIRTYLDWLITLPWKSETADHIDIKQARAILDEDHYDLEKIK
                     DRIVEYLAVGKLKKRLAGPILCFVGPPGVGKTSLGQSIARAMGRKFVRLSVGGVRDEA
                     EIRGHRRTYIGAMPGTIVRAIRDAGTRNPVLMIDEIDKVGADFRGDPQSALLEVLDPE
                     QNNNFRDHYLDLPFDLSQVLFICTANSLDTISPPLRDRMEIIALPGYTEMEKLQIAKR
                     YLLKKQRAANGVNQVQAQITDSALRAIINDYTREAGVRNLEREIG"
BASE COUNT          533 a          884 c          872 g          435 t
ORIGIN      
        1 gtggtgcttc gtcgcgccgc acgcgaatca tcgaagcgcg gcgtgcgaga aaaagcgcgc
       61 gcccgacgcg gaaatacggg acgctgctgc gaatgctctt cgccatgatt gcagccatcg
      121 tacttaccgc cctcacgctg gcgcaggcct cgccctcgga gaccgttccg atgacgcccc
      181 tcccggcaac cgctccgctc gagagcggag cgtgcctgga ttcgtcgcgt ccggttccgc
      241 gcgtgcttca gccgccggtc cagcgttcca accaggtcgt ccgaatcgac aaggtcgagt
      301 cgaccgcgac gctgctcaac ggtgaagtga tcggtttctt gtacaccttg gcagacggat
      361 ccacgtggct aggccagcgg acgcagccgt acatgtcggg cgccgccgcg gcggccgtca
      421 atcaggtgct cgcgttcacg cacatgccgg gcgaaaacgt ttccaaattc ccgccccaga
      481 tggcgtacgg cgtggcgacc aagtacacgc agttcttccg ggtccagatt gcggcgagcg
      541 ccccggcgac gctccagatt caggtcgtcc cgtgcgtggt ctggccggcc gggcgcccgc
      601 tacccgaccc gataatgtag cggccggcgg caacttagca cccggcgcat ccgagtgcta
      661 gaatctggag ttatggggtt cgtgctgagg accaggatcg gggcgttcga gccgaacgcc
      721 gacgttttcg tcgacgagga gggcggccgc gtggtggcgg tcgtcgagat cgccggcgcc
      781 gatcccgaca cgcttcacgt cggcgtcgac gcgcgggacc tgtatatcgg cgggcagcgc
      841 ctcgaggcag tccggctgcg gcgcggctcg ttcgtgcaaa aagaaatcgc gtacggcgag
      901 ttcgtcaagc gcattccgct gccggtggcc gtcgagttcg acggcgttgc cgccagctac
      961 gccgacggat tgctggtcgt ggccctgccg atcgcggcaa cggcttggcg gccgaccgcg
     1021 cgtaccgaac tccacatttt ggtaaagagg acccacccct aagatcatgc ccgaaaaaaa
     1081 atccacgatc gtccccgcga acctgatcgg cattctgccg ctgcaggagg cggtgttgtt
     1141 cccgcacacc gtcatcccgc tggccgtcgt caagaagccc gggatacagc tggttgagga
     1201 agcgctgcgc gaaggcaagc cgatcggcct gtcggtgctg aaggaccgcg agatcgagaa
     1261 cccggggccc gacgacgtgc accgcgtcgg cacgatcggc atcattcaga agatgctgaa
     1321 ggttcccgac ggcacgctgc gctgcatcgt cgccggccaa caggtcttca aggtcgagca
     1381 gttcacgcaa gtcgctccgt acatggtcgc cgcgtacacc gagctgcccg acgtcacggt
     1441 cgaaaacgaa cagctggtcg ccatgcagcg caatctcggc ggcctcttcc agaagctgct
     1501 ctcgtacctg ccgcaagcgc cgcgcgagat ggagatggaa gtccagaaca tcacggatcc
     1561 ggcggtgctc acgtacttcg tggcgtcgac catgcggctc gaaacggccg accgtcaagc
     1621 gctgctggag gagcgcgata ccgccaagcg gatgcgcaag ctcacgctgc tgctcaccaa
     1681 agaactcgag gtcgtcgagc tcggacacaa gattcagagc gacatccagc gcgagatgga
     1741 aaagaaccag cgcgagttct acctccgcca gcagttgcgc gcgattcagg aagagctcgg
     1801 cgaagtcgat ccgcagcttg ccgagctcaa cgaactgcgc gagaaagtcg acgccgccgg
     1861 gatgcccgag gacgtgaaaa aagcggccga ccgcgagctg gaccggcttt cgaaggtgcc
     1921 gcaagcgagc cccgagtaca gcgtcatccg cacgtatctc gactggctga tcacgctgcc
     1981 gtggaagagc gagacggcgg atcacatcga catcaaacag gcgcgggcga ttctcgacga
     2041 ggaccactac gatctcgaga agatcaagga ccggatcgtc gagtacctcg ccgtcggaaa
     2101 gctcaagaag agactcgcgg ggccgatcct gtgtttcgtc gggccgccgg gcgttggaaa
     2161 gacgtcgctc ggccagtcga tcgcgcgcgc gatgggccgc aaattcgtgc gtctgtcggt
     2221 cggcggcgtg cgcgacgaag ccgagatccg cgggcaccgg cggacgtaca tcggagcgat
     2281 gccgggtacg atcgtccgcg cgattcgcga cgccgggacg cgtaacccgg tgctgatgat
     2341 cgacgaaatc gacaaggtcg gcgccgattt ccgcggcgat ccgcagtcgg cgctgctcga
     2401 agtgctcgac cccgaacaga acaacaactt ccgcgatcac tacctcgatt tgccgttcga
     2461 cctgtcgcaa gtgctgttca tctgcaccgc caacagtctc gacaccatct ccccgccgct
     2521 gcgcgatcgc atggaaatca tcgcgctccc gggttacacc gagatggaga agctgcagat
     2581 cgccaagcga tatctgctca agaagcagcg cgccgccaac ggcgtgaacc aggtacaggc
     2641 ccagatcacc gattcggcgt tgcgcgcgat catcaacgat tacacccgcg aggcgggcgt
     2701 gcgcaacctc gaacgcgaga tcgg
//
LOCUS       DASUCJ010000394         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_324916, whole genome shotgun sequence.
ACCESSION   DASUCJ010000394 DASUCJ010000000
VERSION     DASUCJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324916"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1095
                     /locus_tag="VFU90_04720"
     CDS             37..1095
                     /locus_tag="VFU90_04720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479111.1"
                     /translation="MSILPDYGSSPIPAFRQWEAVRALEQPQRVLRTDMYDGHALEIS
                     IRTMREEDVPQADRIMRLAFGTYLGMPEPEATMGDRDFVGTRRRANPANAFVAECDGR
                     IVGSNVAARWGSVGFFGPLTVSPELWNRGVAKRLMEPVMDLFAGWNTACAGLFTFADS
                     ERHVGLYQRFGFWPRFLTALMAKPVEPRPAVAAATPFSATAAGERAGMLDACREVAGS
                     VFEGLDLTAEIRAVEDQRLGDTLLLWSGSRLTGFAVCHTGAGTEAGSGTSYVKFAAVR
                     AGAGGDAFATLLYACEAHAAASGAGVLTAGVNLGRHEAYRRLLAQGYRTQMQGIAMHK
                     DNVAGYSVPGAFVIDDWR"
     gene            1107..>2229
                     /locus_tag="VFU90_04725"
     CDS             1107..>2229
                     /locus_tag="VFU90_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479112.1"
                     /translation="MSRQRFPFVAIAGGAALFVLVELLAFYVPLYRALIDPNSTTGAF
                     EGTIAGLGAVRTDPRRDVLVLGDSRIYSGLDPAVASAASGGRLRFVDGSVPGTTPRCW
                     YFFARAVDPASRRFRAIVVPVDTYADDDSAIGSLDGDRRELDLHYIVFRVEPADVAKL
                     AASFGDWHARLTVGANVLLRGPELRDDVQALAANPAARLQALAAARASEPYDPFRAHP
                     RSETLAGLRADFARGRIDAPRGVDAAERAAIAVQVLHVARPSASYALYRRRWLGPIVE
                     RYAAAGVPVIFVRLPTRPLARASAPAASGSLLEMARAPGVRLLPQAPYLALERPALFA
                     DHDHLDARGSRAFSAQLGRDVEGALAAMAPPAADPSRRDP"
BASE COUNT          303 a          770 c          788 g          368 t
ORIGIN      
        1 atctcgccct cgcgttcgac ctcaacgagt tggtgggtga gcatactgcc agactacggg
       61 tcctcgccga ttccggcctt ccggcaatgg gaagccgtgc gcgcgctcga acagccgcag
      121 cgggttttgc gaacggacat gtacgacggc cacgcgctcg agatttcgat ccggacgatg
      181 cgggaagagg acgttccgca ggccgaccgc atcatgcgtc tcgctttcgg cacctatctc
      241 ggaatgcccg aacccgaggc gacgatgggc gaccgcgatt tcgtcgggac gcgccggcgc
      301 gccaatccgg ccaatgcttt cgtcgcggag tgcgacggcc gaatcgtcgg gtccaacgtg
      361 gccgcccgtt gggggagcgt ggggttcttc ggaccgctga cggtcagccc ggaactctgg
      421 aaccgcggcg tggccaagcg cctgatggaa cccgtcatgg acttgttcgc cggctggaac
      481 acggcgtgcg cgggactatt caccttcgcc gacagcgaac ggcacgtggg gttgtatcaa
      541 cgcttcgggt tttggccgcg attcctcacc gccttgatgg ccaagccggt cgaaccgcgg
      601 ccggcggtgg ccgcagccac gccgttttcc gcaacggcgg ccggggagcg cgccggaatg
      661 ctcgacgcgt gccgcgaggt tgccggcagc gttttcgaag ggctggactt gacggccgaa
      721 atccgcgcgg tggaagacca gcggctcggc gacacgctgc tgctgtggag cggaagccgc
      781 ctcaccggat ttgcggtgtg ccataccggt gcgggtaccg aggccggcag cggcacgtcg
      841 tacgtgaagt tcgccgccgt ccgagccggt gcgggcggcg acgcgttcgc tacgctgctg
      901 tacgcgtgtg aagcgcacgc ggccgcttcg ggtgccggcg tgctgaccgc cggcgtcaat
      961 ctcgggcgcc acgaagccta ccgccgcctc ctggcccagg gctatcgtac gcagatgcaa
     1021 ggcatcgcga tgcacaagga caacgtcgcg ggttacagcg ttcccggggc gtttgtgatc
     1081 gacgactggc gctagcgtcg aaccgcttgt cgagacagcg tttcccgttc gtggcgatcg
     1141 ccggcggcgc cgcgctcttt gtgctcgtcg aactgctggc gttttacgta ccgctctacc
     1201 gcgcgctgat cgacccgaac tcgacgaccg gtgccttcga gggtacgatc gcggggctcg
     1261 gggcggtgcg caccgacccg cgccgcgacg tcctggtgct cggcgattcg cgaatttatt
     1321 cgggcctcga tcccgcggtc gcgtcggccg caagcggcgg ccggctgcgg ttcgtcgacg
     1381 gcagcgtccc cgggacgacg ccgcgctgct ggtatttctt cgcccgcgcg gtcgacccgg
     1441 cgagccgccg ctttcgcgcg atcgtcgtgc cggtcgacac ttacgccgac gacgatagtg
     1501 cgatcggcag tctcgacggc gaccggcgcg agctggatct ccactacatc gtatttcggg
     1561 tcgaaccggc cgacgtcgcg aaactggcgg cgtcgttcgg cgactggcat gcgcgcctaa
     1621 ccgtcggcgc caacgttttg ctgcgcggac ccgaactgcg cgacgacgta caggctttgg
     1681 cggcgaaccc ggcggcgcgg ctgcaggcgc tcgccgcggc ccgcgcgagc gaaccgtacg
     1741 atcccttccg cgcgcatccg cgcagcgaaa cgctcgcggg cctgcgcgcc gatttcgcgc
     1801 gcggacggat cgatgcgccg cgcggcgtcg acgcggcgga gcgcgcggcg atcgcagtcc
     1861 aggtgctgca cgttgcgcgt cccagtgcgt cgtacgcgct ctaccgccgg cgctggctcg
     1921 gcccgatcgt cgaacgctac gccgccgccg gagttccggt gattttcgtg cggctgccca
     1981 cgcgtccgct ggcgcgcgcg tccgctccgg ccgcgagcgg atcgctgctc gagatggcgc
     2041 gcgcacccgg cgtgcggctg ttaccgcagg caccgtacct cgcgctcgaa cgtccggcgc
     2101 tgtttgccga tcacgaccat ctcgacgcgc ggggaagccg cgcgttcagc gcacagctcg
     2161 ggcgcgacgt cgagggcgcg ctcgccgcga tggcgccgcc ggctgcggat ccgtcgcgcc
     2221 gcgatccgc
//
LOCUS       DASUCJ010000395         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1349394, whole genome shotgun sequence.
ACCESSION   DASUCJ010000395 DASUCJ010000000
VERSION     DASUCJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349394"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1174
                     /locus_tag="VFU90_04730"
     CDS             30..>1174
                     /locus_tag="VFU90_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479113.1"
                     /translation="MIVVSDQGTAVSVNAGRNWSDWYNQPTGQFYHLAADNRFPYWIY
                     SGQQDSGTVGIASRSDYGAISFRDWHPVGGDERDYDLPDPEDANIVYGTGLGSRISRW
                     DARTGEVQNVSPWPTSAYGKRPTDIKYRYTWISPIAFSAHAPYALYAGAEVLFRTTDK
                     GMHWDTISPDLTGREDGAKHCDGDVPIANATACGYGVIYSIAPSPRTNDEIWIGTDNG
                     LIQMTADGGKNWRNVSPNNIPKWSKIASLDLSAQESGTAYAAVDNHRQDEFHPIAVRT
                     HDNGKTWTKITNGLPDGHFVAVVRADPVRKGLLYAGTDTGVYVSFDDGDAWQPLQRNL
                     PVAWVRDLLVHGNDLIAATQGRAIWVLDDVSPLRQIDASLAHADAHL"
BASE COUNT          226 a          440 c          354 g          154 t
ORIGIN      
        1 tctggatcaa cccgaagcat ccggaccaca tgatcgtggt cagcgaccag ggcaccgccg
       61 tcagcgtcaa cgccggccgc aactggagcg actggtacaa ccagccgacc ggccagttct
      121 accacctcgc cgccgacaac cgcttcccgt actggatcta ctcgggccag caggattccg
      181 gcaccgtcgg catcgcgagc cgcagcgact acggcgcgat ctcgttccgc gactggcacc
      241 cggtcggcgg cgacgagcgc gactacgacc tgccggatcc cgaagacgcg aacatcgtct
      301 acggcacggg cctcggctcg cgcatctcgc gctgggacgc gcgaaccggc gaagtgcaga
      361 acgtctcgcc gtggccgacg tccgcgtacg gcaagcggcc gaccgacatc aaataccgct
      421 acacgtggat ctcgccgatc gcgttctccg cgcacgcgcc gtacgcgctc tacgcgggcg
      481 cagaggtgct gttccgcacc accgacaaag gcatgcactg ggacacgatc agccccgacc
      541 tcaccggcag agaggacggc gcgaaacact gcgacggcga cgtgccgatc gcgaacgcga
      601 cggcctgcgg ctacggcgtc atctactcga tcgcgccgtc gccgcgcacc aacgacgaaa
      661 tctggatcgg caccgacaac ggcctgatcc agatgaccgc cgacggcggc aagaactggc
      721 gcaacgtctc gccgaacaac atcccgaagt ggtcgaagat cgcctcgctc gatctctccg
      781 cgcaggaatc cggcaccgcg tacgccgccg tcgacaacca ccggcaggac gagttccacc
      841 cgatcgccgt gcgcacgcac gacaacggca agacctggac gaagatcacg aacggccttc
      901 cggacggcca tttcgtcgcc gtcgtccgcg ccgatcccgt caggaaaggc ttgctctacg
      961 ccggcaccga caccggcgtc tacgtctcgt tcgacgacgg cgacgcatgg caaccgctgc
     1021 agcgcaacct gccggtcgcg tgggtgcgcg acctgctcgt ccacgggaac gacctcatcg
     1081 ccgcgacgca aggccgcgcg atctgggtgc tcgacgacgt gtcgccgctc cgccagatcg
     1141 acgcgagcct cgcgcacgca gacgcgcacc tctt
//
LOCUS       DASUCJ010000396         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_691838, whole genome shotgun sequence.
ACCESSION   DASUCJ010000396 DASUCJ010000000
VERSION     DASUCJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691838"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFU90_04735"
     CDS             <1..286
                     /locus_tag="VFU90_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479114.1"
                     /translation="DFLFAHRPAAANLLGNAEVKETGLGRLRVVSAEGLIGFKLQAFV
                     NNPKRTQDIEDIRSLLRVNQHSLNMSEVKGYFALFGREALLDEIIADLDR"
     gene            309..452
                     /locus_tag="VFU90_04740"
     CDS             309..452
                     /locus_tag="VFU90_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479115.1"
                     /translation="MREAGVAYRPSPARDPFDAWISLMEAVEALCPRWPAREHRLTGE
                     FKL"
     gene            463..1176
                     /locus_tag="VFU90_04745"
     CDS             463..1176
                     /locus_tag="VFU90_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5479116.1"
                     /translation="MSIELVGFDGDDTLWHSEGYYQNAAAEFERIVGAWIDVRDARVR
                     ERLHAVERRNLKLFGYGAKGMTLSMVEAAIELTDSRIGAQDIHRIVALGRRVLEHPVE
                     LLPGVRAAVEAVARDHRVVLITKGDLFHQESKVERSGLGDLFHRIEIVSEKNEASYAR
                     VLDEFQVAAPAFAMIGNSLRSDIEPVIGLGGWGIHMPYHVTWAHELQHGIESGHPRVI
                     AIGSAAEIADAVARCGQES"
BASE COUNT          224 a          376 c          393 g          209 t
ORIGIN      
        1 ggatttcctg ttcgcgcacc gtcccgctgc ggcgaatctg ctcggcaatg ccgaagtgaa
       61 agagaccggg cttggccgtt tgcgtgtcgt gtcggcggaa ggcttgatcg gattcaagct
      121 gcaggcgttc gtcaacaatc cgaaacgcac acaggatatc gaggacattc gttcgctgtt
      181 gcgcgtcaac cagcactcac tcaatatgtc cgaagtgaaa ggttactttg ccctgttcgg
      241 acgcgaggct ctgctcgatg aaatcatcgc cgatctcgac cgctgacgat tcacgacgcg
      301 gattcttcgt gcgcgaggcc ggcgttgcgt atcggccttc gcccgcgcgc gatccgttcg
      361 atgcatggat ttcattgatg gaagccgtcg aagcgctgtg cccgcgctgg cccgcgcgcg
      421 agcaccggct gacgggcgaa ttcaagttgt gagcggatcg gagtgagcat cgaattggtc
      481 ggcttcgacg gcgacgacac gctctggcac agcgagggtt actaccagaa cgccgccgcc
      541 gagttcgagc gcatcgtcgg cgcgtggatc gacgtccgcg acgcgcgcgt gcgcgaacgc
      601 ctgcacgcgg tcgagcggcg caatctgaaa ttgttcggct acggcgccaa gggcatgacg
      661 ctgtcgatgg tcgaggcggc gatcgagctg accgacagcc gcatcggcgc gcaggacatc
      721 caccgcatcg tcgcgctcgg ccgccgcgtg ctcgaacatc cggtcgaact gctgcccggc
      781 gtccgcgccg cggtcgaagc ggtcgcgcgc gaccatcgcg tcgtgctgat caccaagggc
      841 gacctgttcc accaggaaag caaggtcgag cgcagcggac tcggcgacct gttccatcgc
      901 atcgagatcg tgtcggagaa gaacgaggcc agctacgcgc gcgtgctcga cgaattccag
      961 gtcgccgcgc ccgcattcgc gatgatcggc aactcgctgc gttcggacat cgagccggtc
     1021 atcggcctcg gcggctgggg catccacatg ccgtatcacg tgacctgggc gcacgaactc
     1081 caacacggca tcgaaagcgg ccatccgcgc gtgatcgcga tcggatcggc agccgaaatc
     1141 gcggatgcgg tcgcgcgctg cggccaagaa tcataacgaa acggagtgag cgacatgggc
     1201 at
//
LOCUS       DASUCJ010000397         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1013066, whole genome shotgun sequence.
ACCESSION   DASUCJ010000397 DASUCJ010000000
VERSION     DASUCJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013066"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>2180)
                     /locus_tag="VFU90_04750"
     CDS             complement(108..>2180)
                     /locus_tag="VFU90_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEU5479117.1"
                     /translation="AGLTRDQLVGAYRTMLLSRRLDDKEIQLKGQNKIFFQISGAGHE
                     AVLTAAGMALRPGHDWFFPYYRDRALCLQLGMTPAEMLYEAVGAARDPNSGGRQMPSH
                     WGSKRLNIVSSSSPTGTQFLHATGCAEASLRASLLSITEGFESDEVTYVSAGDGTTSE
                     GEFWESLNTSCNLGLPVVYLIEDNGYAISVPVEVNTPGGNISKLLAAMPGLFIEEVDG
                     CDVVASYAAMSKAVAYARARRGPALVHAHVIRPYSHSLSDQETMYRPPEEREADAARD
                     PLVTFPKWLVAEGHISDKDLEALKAEVDAEILAATDDALAQPQPAADTVLYAVYSPDV
                     DPTSEQFDTEDDPKFSGEPTTMVDLLNACMKDEMHRDGNILVFGEDVADASRSQYLGK
                     VKGKGGVFKVTWGLQKEFGGDRVYNSPLAEANIAGRAVGLAVRGFKPVVEIQFFDYIW
                     PAYMQIRNELATMRWRSNNEFSAPAVVRVTYGGYIRGAIYHSQTGASLFTHCPGLRVV
                     CPATALDANGLLRTAIRCDDPVIFLEHKHLYRQTYNKGRNPGPNFMIPFGKAKILREG
                     AGITLVTYGATVQRALVAANKVAEDGIDVEVIDLRTLSPWDRDAVMTSVRKTSRVIVA
                     YEDSLSWGYGAEIAAAIADECFAWLDAPVKRVASTDTFVGYAPSLEDAILPQTDDFVR
                     AYRELAAF"
BASE COUNT          376 a          744 c          684 g          376 t
ORIGIN      
        1 cactcgccgc cgccgctaca ccagccagtt agccggatta tcggccggat gcaacgaagc
       61 ccgcgcggac gcgggcctcg tgccgcctaa cggaccaccg ccggcagtca gaacgccgcg
      121 agctcccggt aggcgcggac gaaatcgtca gtctgcggca ggatcgcatc ctcgagcgac
      181 ggcgcatagc cgacgaacgt gtcggtggac gccacgcgct tcaccggcgc gtcgagccac
      241 gcaaagcact cgtccgcgat cgcggccgcg atctccgcgc cgtagcccca cgagagcgaa
      301 tcctcgtagg ccacgatcac acgcgatgtc ttgcgcaccg acgtcatcac cgcatcgcga
      361 tcccacggcg acagcgtgcg caaatcgatc acctcgacgt cgatgccgtc ctccgccact
      421 ttgttcgccg ccaccagcgc gcgctgcacc gtcgcgccgt acgtcaccaa cgtgatgccc
      481 gcgccctcgc gcagaatttt cgccttcccg aacggaatca tgaagttcgg cccgggattg
      541 cggcccttgt tgtacgtctg gcgatagagg tgcttgtgct cgaggaaaat caccggatcg
      601 tcgcagcgaa tcgcggtgcg caacagtccg ttcgcatcga gcgcggtggc cgggcagacg
      661 acgcgcaacc ccggacaatg cgtgaacagc gacgcccccg tctgcgagtg atagatcgcg
      721 ccgcggatgt agccgccgta cgtcacgcgc accacggccg gcgcgctgaa ttcattgttc
      781 gaacgccagc gcatggtcgc gagttcgttg cgaatctgca tgtacgcggg ccagatgtag
      841 tcgaagaatt gaatctcgac caccggcttg aatccgcgca ccgccaatcc aaccgcgcgg
      901 ccggcgatgt tcgcttcggc taacggagaa ttgtagacgc ggtcgccgcc gaattccttc
      961 tgcaagcccc acgtcacctt gaacacgccg cccttgcctt tcaccttgcc gaggtactgc
     1021 gagcgcgacg cgtcggccac gtcctcgccg aacacgagaa tgttcccgtc gcggtgcatt
     1081 tcatctttca tgcacgcgtt gagcagatcg accatcgtcg tcggctcgcc cgaaaacttg
     1141 ggatcgtcct cggtgtcgaa ctgttcgctc gtcgggtcga cgtcggggga gtacaccgcg
     1201 tagagcaccg tgtcggccgc cggctgcggc tgggcgagcg cgtcatccgt cgccgcgaga
     1261 atctccgcgt cgacctcggc tttgagcgcc tcgaggtcct tgtcgctgat gtggccttcg
     1321 gccacgagcc acttcgggaa agtgactaac ggatcgcgcg ctgcgtccgc ttcgcgctcc
     1381 tcgggcggcc ggtacatcgt ttcctgatcg gacaacgagt gcgagtacgg ccggatcacg
     1441 tgcgcgtgca ccaacgccgg cccgcggcgt gcgcgcgcgt acgcgacggc cttgctcatc
     1501 gccgcgtagc tggccaccac atcgcagccg tccacctcct cgatgaacag ccccggcatc
     1561 gccgcgagca gcttcgagat gttgccgccc ggcgtattga cctcgaccgg caccgagatg
     1621 gcgtagccgt tgtcttcgat gagataaacg acgggaaggc cgaggttgca ggaggtattg
     1681 agcgattccc agaactcgcc ttcgctggtc gtgccatcgc ccgccgacac gtacgtgact
     1741 tcatcggact cgaagccttc ggtgatcgag agcagcgatg cgcggagcga cgcttcggcg
     1801 catccggtgg cgtgcagaaa ctgcgtgccg gtgggcgacg atgacgacac gatgttgagg
     1861 cgcttgctgc cccagtggct cggcatctgc cggccgccgg aattggggtc gcgcgcggcg
     1921 cccaccgcct cgtacaacat ctcggccggc gtcatgccta actggagaca cagggcgcgg
     1981 tcgcggtagt acgggaagaa ccagtcgtgc ccgggccgca gcgccatgcc ggcggccgtc
     2041 aacaccgctt cgtgaccggc gccggagatc tgaaagaaga tcttgttttg tcccttgagc
     2101 tggatctctt tgtcgtccag ccggcgagac agcagcatcg tacggtacgc gcccacgagc
     2161 tggtcgcgag tcaggccggc
//
LOCUS       DASUCJ010000398         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1257645, whole genome shotgun sequence.
ACCESSION   DASUCJ010000398 DASUCJ010000000
VERSION     DASUCJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257645"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..>1492)
                     /locus_tag="VFU90_04755"
     CDS             complement(136..>1492)
                     /locus_tag="VFU90_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479118.1"
                     /translation="AFGLAGAVVGVYWERLEFAGSHLATMMFRASGFNSATHVGGAYL
                     EATLAMLVPFGLAAAAIVDRNGLKAWWYGIVVASAGAVLMTLSRAAVAAWLIGVVAFA
                     VLARLKSTTPASNVGALRSWWRWAFAAACLIVLPFVAGQSELLWGRLGTSRTDLEVRV
                     GHWRDALGLMRERPGSALFGMGLGSFPRDFYLANAERMQLPAYRLSTDASGGLPYLEL
                     LGGQGMYVDQRVSAGSARTSRLTGEVRSSAPGAAIAASLCEKSMLNSARCTFARIRAT
                     TNWSRFDVDLPSPRGATSGFVPAMPLAFSLANPQFGTRVEVTELSLRDGRRELLRNGS
                     FRHGLDHWFMTSDDHLAWHTKNTALEIFFEQGALGILAWLALAISAVAIVLEAGRRPA
                     ATAAFAGSVVAFLAVGTFDSILDSPRIVVLIALIVVTGWLAVRATHLPRRSESATEGP
                     S"
BASE COUNT          294 a          509 c          472 g          217 t
ORIGIN      
        1 cagaccgacc tgctcgtttc gcctggcata gggttgctgc gctggattcg taatgcgatt
       61 cgatcgttac gctgaaatgt gaaatgacgt cggtaaacgt cggtactgaa gtgctcgagg
      121 gactcgttgc gcgtctcagg atggaccctc ggtagcggat tcggatcgtc gcggcaggtg
      181 ggttgcgcgc acggcaagcc atcccgtgac cacgatcaag gcgatcagga cgacgatccg
      241 cggcgagtcg aggatgctgt cgaacgttcc gacggccagg aacgcaacga ccgaacccgc
      301 aaaggccgcc gtcgcggccg gccgccgccc ggcctcgagg acgatcgcga cggcagagat
      361 cgccagcgcg agccacgcga ggatgcccag cgcaccctgt tcgaagaata tttcgagcgc
      421 cgtgttcttc gtgtgccagg ccagatggtc gtccgacgtc atgaaccagt ggtccaggcc
      481 gtggcgaaag gagccattcc gaagcagctc ccgccggccg tcacgcaacg acaattcggt
      541 cacttcgacg cgcgtcccga attgcgggtt ggcgagcgag aatgctagcg gcatcgcggg
      601 cacgaacccg gatgttgcgc cccgcgggga tggcaggtcg acgtcgaagc gactccagtt
      661 ggtcgttgcc cggatccgcg cgaaggtgca gcgagcagaa ttcaacatcg acttctcgca
      721 caggcttgcg gcaatggccg cacccggagc acttgagcgt acttcgccgg tcaggcgcga
      781 cgtgcgcgcg gatccggcac tgacccgctg atcgacgtac atcccttgcc ccccgagcag
      841 ttccaggtac ggcagcccgc cgcttgcatc ggtcgagaga cggtaagcgg gcaactgcat
      901 ccgttccgcg ttcgcgagat agaaatcgcg cgggaagctt ccaagaccca ttccgaacag
      961 cgccgagccc gggcgctcgc gcatcaaccc gagcgcgtcg cgccagtgcc cgacgcgtac
     1021 ttcgagatcc gtgcggctgg tgccaaggcg cccccacaag agctccgact gcccggcgac
     1081 gaacgggagc acgatcaggc atgctgcggc gaacgcccat cgccaccatg accggagtgc
     1141 tcccacattc gaagccggcg tggtcgattt cagccgagcc agcacggcga acgcgaccac
     1201 tccgatcagc cacgcggcaa ccgcagcccg tgacagcgtc atcagcaccg cgccggcgct
     1261 cgcgacgacg atgccatacc accacgcctt cagcccgttc cggtcgacga tcgccgcagc
     1321 cgcgagaccg aacggcacca gcatcgcgag cgtcgcctcc aggtaggcac cgccgacatg
     1381 cgtggccgaa ttgaaaccgc tcgctcggaa catcatcgtc gcaaggtgcg aaccggcgaa
     1441 ctccaggcgc tcccagtaga cgccgacaac cgcaccggcg agaccgaacg cc
//
LOCUS       DASUCJ010000399         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_814422, whole genome shotgun sequence.
ACCESSION   DASUCJ010000399 DASUCJ010000000
VERSION     DASUCJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814422"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..702)
                     /locus_tag="VFU90_04760"
     CDS             complement(100..702)
                     /locus_tag="VFU90_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5479119.1"
                     /translation="MRATRPSNTALAAAQQLTELESCTLGVIRQCQPCSTYRVRSEFA
                     RSSTTAWSASAGAIYPVIERLVRAGYVRIRRRSTDSRGRRDLALTRRGARAVQDWIMQ
                     LEPSIARSTPDPIRSRVCFLDQLASDGDRQEFLTLAEQLTGLMISDLRAQERSTGDMT
                     EIDRLVVIGSRLQLEARLAWLGIVRRRLGLPRTPTTAGRA"
     gene            777..>1177
                     /locus_tag="VFU90_04765"
     CDS             777..>1177
                     /locus_tag="VFU90_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HEU5479120.1"
                     /translation="MHPAWMVTVPLLFAWPSPRPAIAQMPAAREAQTAARDARLRVTP
                     VLVAPGFPVHVVITGLPPGAAATVHAQSVVTDDSGHLQPYYADATFLGNRGRTVSLDT
                     AAPVTGYYRGADPRGLFWSARPTARDSAARTA"
BASE COUNT          189 a          414 c          382 g          192 t
ORIGIN      
        1 agccagatcc gtgcgctcgc cgtggatcag atggccgacg acgccgaaca cgtcccatgt
       61 gttctcgcct tcgtcgtgcg acgtcggggg gtaccatgct caagctctcc cagctgtggt
      121 cggagtccgc ggaagcccga ggcgccgtcg cacgatgcca agccatgcga gacgcgcctc
      181 cagctgcaac cgactaccga tgaccacgag ccggtcgatc tccgtcatgt cgcccgtcga
      241 tcgttcttgc gcccgcaggt cgctgatcat cagaccggtg agctgttcgg ccagcgttag
      301 aaattcctgg cgatcgccgt cggacgcgag ctgatcgaga aagcagacgc gcgaccggat
      361 cggatcgggc gtcgatcgcg cgatcgatgg ttcgagctgc atgatccagt cttgcaccgc
      421 acgcgcgccg cgcctggtga gcgccaggtc gcgccggccg cgcgaatccg tggagcgccg
      481 gcggatgcga acatacccgg cccgcaccaa gcgctcgatg accgggtaga tcgcaccggc
      541 gctggcactc cacgccgtcg tgctggagcg tgcgaattcg ctgcgcaccc gataggtgga
      601 gcacggctgg cattggcgga tcacgccgag ggtgcaactc tcgagctccg tcaattgctg
      661 cgccgccgcg agggcggtgt tcgatggtct cgttgcgcgc atagtacgga tagtactata
      721 ttgacgccgg cgttggaagg ccggtgagct tccccgatga tcacaggaaa gccgcaatgc
      781 acccagcatg gatggtgacg gttccgctgc tgttcgcctg gcccagcccc cgccccgcca
      841 tcgcacaaat gccggccgcg cgcgaggcgc aaaccgcggc acgcgacgct cgcctgcgcg
      901 tcacgccggt tctggtcgca ccggggttcc ccgttcacgt cgtcatcacc ggcctgccac
      961 ccggcgccgc ggcaacggta catgcgcagt ccgtcgtcac tgacgactcc ggccacctgc
     1021 aaccgtatta cgccgacgcg acgttcctcg gcaaccgcgg ccgcacggtc tcgttagaca
     1081 ccgccgcgcc ggtcacggga tactatcgcg gcgccgatcc gcgcggattg ttctggtcgg
     1141 cgcgaccgac ggcgcgcgat tccgccgcgc ggacagc
//
LOCUS       DASUCJ010000400         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1272951, whole genome shotgun sequence.
ACCESSION   DASUCJ010000400 DASUCJ010000000
VERSION     DASUCJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272951"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFU90_04770"
     CDS             <1..1146
                     /locus_tag="VFU90_04770"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479121.1"
                     /translation="STDAGKTWTHLGIDDTQAISKIRVDPRNADIVYVAAFGHPYGRN
                     ENRGVFKSTDGGKTWRKVLYRDDKTAPIDVEIDPSNSNVLYAALWEAYRTPWSMSSGG
                     PGSGLFKSTDAGETWSEITRNPGLPTGVIGKIGISVSPVDPNRVYAIVENANGGVFRS
                     DDAGATWKLVNDERKLRQRAFYYTHVVADTKLRDRVYVLNVSFFRSDDGGVHFDSTVN
                     VPHGDNHDFWIAPNDNQRMIEANDGGGTVSVNAGKTWTAEDFPTAQLYHVAVTTDFPY
                     HVCGAQQDNTTLCLPSNDWSNLRGASSRRLGDWMYEVGGGESGYIAPDPKDPNVFYAG
                     SQGAPPRNRHRQRPDAATIATRRGVSLPAMMVRRSSSLPRRTSRARH"
BASE COUNT          233 a          448 c          400 g          187 t
ORIGIN      
        1 tccaccgacg ccggcaagac ctggacacac ctcggcatcg acgacacgca ggcgatctcg
       61 aagatccgcg tcgacccacg caatgccgat attgtgtatg tcgccgcgtt cggccatccg
      121 tatggacgca acgagaatcg cggcgtcttc aagtccaccg atggcgggaa gacctggcgc
      181 aaggtgctct atcgcgacga caagacggcc ccgatcgacg tcgaaatcga tccctccaac
      241 tcgaatgtgc tctacgccgc gctgtgggag gcgtaccgca cgccgtggtc gatgtcgagc
      301 ggcggcccgg gaagcgggtt gttcaagtcc actgacgccg gcgaaacgtg gtccgagatc
      361 acgcgcaacc cggggctccc cacgggcgtc atcgggaaga tcggcatctc cgtctccccc
      421 gtcgatccga accgggtcta cgccatcgtc gagaacgcga acggcggcgt ctttcgctcc
      481 gacgacgccg gcgccacctg gaagctcgtg aacgacgagc gcaagctgcg ccagcgcgcg
      541 ttctactaca cgcacgtcgt cgccgacacc aagctcaggg accgtgtcta cgtcctgaat
      601 gtgagcttct tcagatcgga cgacggcggc gtccacttcg attccaccgt caatgtgccg
      661 cacggcgaca atcacgactt ctggatcgcg ccgaacgaca atcagcggat gatcgaagcc
      721 aacgacggcg gcggcacggt ctcggtgaac gccggcaaga cgtggacggc cgaagacttc
      781 cccacggcgc agctctacca cgtcgccgtc accaccgatt tcccgtatca cgtgtgcggc
      841 gcgcagcagg acaacacgac gctctgcctc ccctcgaacg actggtcgaa cctgcgcggc
      901 gccagttcgc gccggctcgg cgactggatg tacgaggtcg gcggcggcga atcgggctac
      961 atcgcccccg acccgaaaga cccgaacgtc ttctacgccg gcagccaggg cgcgccgccc
     1021 cgaaatcgcc atcgccagag gccagacgcg gcgacgatcg cgactcgacg gggtgtgtcg
     1081 ctgccggcga tgatggtgcg ccggtcctct tccctgccgc ggcgcacctc gagagcgcgc
     1141 cactgaccga cgggcgcggt ggcccccacg gttatcggcg ggagactgtc ccacgggagg
     1201 ccgctcagag cgggagcgag aggcgaaggc ggcgcgtcgg ccgcgtacca ctcgccttcc
     1261 ccgctttc
//
LOCUS       DASUCJ010000401         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_554593, whole genome shotgun sequence.
ACCESSION   DASUCJ010000401 DASUCJ010000000
VERSION     DASUCJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554593"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFU90_04775"
     CDS             complement(<1..649)
                     /locus_tag="VFU90_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5479122.1"
                     /translation="MLPLLVAAMIVYGQCAVAQSANAASPKTFYRPADLAWTGAFALG
                     SFAISRVDPAVAKYFQEPQHQTDPVMRESADVFTHLQETTLTLGGLATYGIARLVGAG
                     RDVQEIGLHAAEAIAAASITSQVIRGPLGRARPKDATPKFEDQYEFHWFNGFRNFKYR
                     AYPSIHASSSFAAATVVVAETHFRSPRSTWIVAPLAYGIAAGPGYARMYLGQHWAS"
     gene            complement(742..1182)
                     /locus_tag="VFU90_04780"
     CDS             complement(742..1182)
                     /locus_tag="VFU90_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5479123.1"
                     /translation="MSKGRVLLAHGNADCRKIYGSVLAFSGYDVEVVADGESALRQLE
                     TLSFDIVVSDLYLSSDGDECLPRTVRTNPHTAHLPLVVITGWMTEPHRRLALDVGADA
                     FLALPTRPRDLVEIVARLLEQPSVPLTSIDVVNDPRNHTVANGH"
BASE COUNT          251 a          460 c          489 g          230 t
ORIGIN      
        1 cgctcgccca gtgctggccg aggtacatcc gcgcgtagcc cgggccggcg gcgattccgt
       61 acgcgagcgg ggcgacgatc cacgtcgagc ggggggaccg gaaatgggtc tcggcgacga
      121 cgaccgtcgc ggccgcgaac gacgaggacg cgtggatcga cggatacgcg cgatatttga
      181 agttgcgaaa cccattgaac cagtgaaact catactggtc ctcgaacttc ggcgtcgcgt
      241 ccttcggccg cgcgcgcccg agcgggccgc ggatcacctg actcgttatg ctcgcggcgg
      301 cgatcgcttc cgcggcgtgc agcccgatct cctgcacgtc gcgaccggcg ccgaccagac
      361 gcgcgattcc gtacgtcgcc agcccgccaa gcgtcagcgt cgtctcctga agatgcgtga
      421 agacgtccgc gctctcgcgc atcacggggt cggtctgatg ctgcggttcc tggaaatact
      481 tcgcgacagc gggatcgacg cgagagatgg cgaacgagcc gagtgcgaag gcgccggtcc
      541 acgccaggtc ggcgggccga tagaaggtct tcggcgacgc ggcgttcgcg ctctgcgcga
      601 cggcgcattg cccgtaaacg atcatcgccg cgacgagaag cggaagcaca cgacgagaac
      661 gcgcgagcgg caaacccgtg cctcgatgag agtcccgccg gctaaatgca gccgacgtgc
      721 cgggcgagcc ttgtcgcgcg ctcagtgccc gttggcgacc gtgtgattgc gcgggtcgtt
      781 gacgacgtcg atggaggtca gcggcaccga tggctgctcg aggaggcgcg cgactatctc
      841 aacgagatct cgtggtcggg tggggagggc cagaaaggca tcggcgccca catcgagggc
      901 cagtcgccgg tgcggctccg tcatccagcc agtgatcaca acgaggggga gatgcgccgt
      961 gtgcggattc gtgcgcaccg tccgcggcag gcactcgtcg ccatcgctgg agaggtagag
     1021 atccgagacg acaatgtcga aggagagcgt ctcgagctga cgcaacgcac tctctccgtc
     1081 ggctacgact tccacgtcat agccgctgaa cgccagcacg ctgccgtaaa tctttcggca
     1141 gtcagcgttt ccgtgagcga gcagaacgcg tcccttggac atccgctttc ccgacgggtt
     1201 ggggagctgc ggcgtcgcat tggcaccata tgtccaaaca caaggcgccg caagcaggcg
     1261 caaggcctct tcacgcgact gctatcaacc cgttacgacg cgggatgttc cggtgcctgc
     1321 gccctaccgc cctcggcggc cacgagcggc atccagctct cggcggacca gtcggtggtc
     1381 agcagattga tggtgtgctg ctcctccagg gggtgttcgg ccgtgtcgac
//
LOCUS       DASUCJ010000402         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_463023, whole genome shotgun sequence.
ACCESSION   DASUCJ010000402 DASUCJ010000000
VERSION     DASUCJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463023"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFU90_04785"
     CDS             <1..374
                     /locus_tag="VFU90_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479124.1"
                     /translation="AGYLLRMILDAPSPAPISSLDIVSRKSKDRLVSEPPEAIYGGYA
                     NARGETLLFGKELVYRVNFKNRTIQQRPKLNPAAQARYDATEAGENFEYCNLMFRRLS
                     GAEIVEICASGAFRVTPKLLN"
     gene            complement(367..2349)
                     /locus_tag="VFU90_04790"
     CDS             complement(367..2349)
                     /locus_tag="VFU90_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU5479125.1"
                     /translation="MASLTGHGDRRVELTLRGLVLGAGITLVFTAANVYLGLKVGLTF
                     ASTIPAAVISMAVLRAFKSATIFENNIVQTVASAAGTLSAVIFVLPGLVMIGWWTNFP
                     FWESFGVCAIGGVLGVMYSVPLRRALVTRSDLPYPEGVAAAEVLKVGIGGAGTESAAE
                     SNRLGLLTLVVGTVASAGFAAVVAMRVFAGTIAAYVRAGSAATGIGFSLSLALVGAGH
                     LIGLSVGLAILAGLVVAWGIATPVLTALHPAAGPAADVATAVWSHQVRFIGAGAIGVA
                     ALWTLARLAKPVWNGVVSAIAASRRLAGGDENLPRTERDIPIVWVAVISASCLVPLGL
                     LLAAFLHGSLASGTIGPLVIAAVAYVVVAGFLVAAACGYMAGLIGSSNSPVSGLAILA
                     VLGASLLLLALSGRFDAAYGQVLVAYALFVTAVLLCVATISNDNLQDLKTGQLVDATP
                     WRQQAALIFGVVMGAIVIPPILDLLNHAYGFAGAPQHATIGQPLPAPQATLIAALAKG
                     VIQGRIDWGLIGIGATIGLAFVLLDELLRMTGRLRLPPLAVGLGIYLPASTTAPVVLG
                     ALIGWFYNRWASRREQGDRAKRLGVLLASGFIVGESLFGVLLAGLIVVMNTPNPLAVV
                     GDAFQTAATALGVVAFVAAVAGLYWWTARASERSSS"
     gene            complement(2680..3468)
                     /locus_tag="VFU90_04795"
     CDS             complement(2680..3468)
                     /locus_tag="VFU90_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5479126.1"
                     /translation="MNFVNLELILIGLIVGVLVGLTGVGGGSIMTPLLVALLKVNPLT
                     AVGTDLLYSVPTRLYGAFLHRQQGTVNGEIVRALLWGGLPASLLSLALLFWLRHHIDV
                     ALVELWTRRAIGITLFLAAGMILAQPLLKRHGVASLGQPFSWKPGQRARVIALGVVVG
                     FIVTLTSIGSGAVTLPLLALALPLVGLPELIGSDIAFAAFLIPAAALGRWTMGDVNVG
                     LAVNLLIGSLPGVYIGSKLCTRLHARWLRPAVAVTLIFVGSRLI"
     gene            complement(3636..>4369)
                     /locus_tag="VFU90_04800"
     CDS             complement(3636..>4369)
                     /locus_tag="VFU90_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEU5479127.1"
                     /translation="PSEAQIRAKVHDLLDLIQLRNQAGRYPSQLSGGQRQRVALARAL
                     AVEPSVLLLDEPFGALDARVRTELRAWLRRLHDETHTTTVIVTHDQEEALEVADRVAV
                     MNEGRIEQIGTPEEVYNRPATSFVFNFLGSVNLFHGRIDNGTAYIDRTATDHLVFVRP
                     HSLEITRRLPAGVDHFRATTRHINAAGPRVKVEAVTEWGAPVQVEMSQDRFRELSLTK
                     GEEVFVVPTEVAVFRSGGRAPTANP"
BASE COUNT          859 a         1472 c         1375 g          663 t
ORIGIN      
        1 gtgcgggtta cttgttacgc atgattctgg acgcaccgtc acccgccccc atttccagcc
       61 tcgatatcgt cagccgtaag tccaaggacc gtctcgttag cgagccgccc gaagccattt
      121 acggcggcta cgccaacgcg cgcggcgaga cgctcctttt cggtaaggag ctggtgtatc
      181 gggtgaactt caaaaaccgg acgattcagc agcggccgaa gctcaatccg gcggcccagg
      241 cgcgttacga tgcgaccgag gccggcgaga acttcgagta ctgcaacctg atgttccgcc
      301 gcttgagcgg cgccgaaatc gtcgagatct gtgcgtccgg tgcgtttcgc gtgacgccga
      361 agcttctcaa ctagaggaac gctccgaagc ccgcgccgtc caccaataga gtccggctac
      421 ggccgcgacg aacgcgacga cgccgagcgc cgttgcggcc gtctgaaacg catcgcccac
      481 cacggcgagc ggattgggcg tattcataac gacgatcagc ccggcgagca acacgccgaa
      541 cagactctcc cccacgatga acccggaagc gagtaagacg ccaagccgct tggcgcgatc
      601 cccttgctcc cggcgcgatg cccaacggtt gtaaaaccag ccgatcagcg cgccgaggac
      661 cacgggtgcg gtagtcgacg ccggcaggta aattcctaag ccgacggcca gcggcggaag
      721 ccgcaggcgt cccgtcatac gcaagagttc gtcgagcagg acgaaggcga gtccgatcgt
      781 cgcgccgatg ccgatcaaac cccagtcgat ccggccctgt atgacgccct tcgccaatgc
      841 ggcgatcagc gtcgcttgcg gagccggcag cggttgaccg atcgtcgcat gctgcggcgc
      901 accggcaaag ccgtacgcat gattgagcaa gtcgaggatc gggggtatca cgatcgcgcc
      961 catcaccacg ccgaaaatca gggccgcctg ctgacgccac ggcgtcgcgt cgaccagctg
     1021 accggtcttg agatcttgta gattgtcgtt cgagatcgtc gcaacgcata acagcacggc
     1081 ggtcacgaac agcgcgtaag cgacgagcac ctgaccgtag gccgcgtcga aacgtccgct
     1141 cagcgcaagc aggagcaacg aagcaccgag aacggccaag atggcaagtc cggagaccgg
     1201 gctattcgag gagccgatca gccccgccat atatccgcag gccgccgcga cgaggaatcc
     1261 ggcgacgacc acgtaggcga cggcagctat caccagcgga ccgatcgtcc cggatgcgag
     1321 gcttccgtgg aggaaagccg cgagcagcag ccccagcggc acgaggcacg acgcgctgat
     1381 aacggcgacc cacacgatcg gaatgtcgcg ctcggtgcgc ggaaggtttt catcgcctcc
     1441 ggcgaggcgc cgcgaagcgg cgattgccga aacgacgccg ttccacaccg gtttcgccag
     1501 ccgcgcgagc gtccacagcg ccgcgacgcc gatcgcaccg gctccgatga aacgtacctg
     1561 atgcgaccag accgcggtcg caacgtcggc ggccgggccg gcggcggggt gcagcgcggt
     1621 cagaaccggc gtcgcgatgc cccaggcgac gaccagcccg gcgagaatcg caagcccgac
     1681 cgagagcccg atcaaatggc cggctccgac gagcgcgagc gacagcgaaa agccgatccc
     1741 ggtcgccgcc gaacccgccc gcacgtaggc ggcgatcgtc cccgcgaaga cgcgcatcgc
     1801 gacgaccgcc gcaaaaccgg cggaggcgac ggttccgacg accagcgtga gcagtcccaa
     1861 gcggttcgac tccgccgccg attccgttcc ggcgccgccg attccgactt tcaagacttc
     1921 ggcggcggca acgccttcgg gatacggcaa gtcggatcgg gtcacgagcg cgcgccgcaa
     1981 cgggacgctg tacatcacgc cgagcacgcc gccgatcgca caaactccga aggactccca
     2041 gaacgggaag ttcgtccacc agccgatcat cacgaggccc ggcagtacga agatcacggc
     2101 cgacagcgta ccggcagccg acgcgacggt ttgcacgatg ttgttttcga agatcgtagc
     2161 gctcttgaac gcacgcagca ccgccatcga aatgacggcc gcagggatag tcgaagcgaa
     2221 cgtcagccca accttgagcc cgagatagac gttggcggcg gtaaacacca gcgtaatgcc
     2281 ggcgccgagc acgagcccgc gaagcgtcag ctcgacgcgc cgatcgccgt ggccggtcag
     2341 actcgccaaa ttgcgctttc gtccatcgcg ggcgcagttc ttcagtgcac gcaacaaagc
     2401 ccgcaacgcg aggctgcagg ctttgcgata gctccggtcg tccgcctgtt tgggaccgtt
     2461 accaatccct caccgacgtg tcgatccggt ttggtccgaa cgctcgaatc gcgctttcgc
     2521 gacgatcgtc aacacacgcg gaccgcggct tttatgttcc gcgcttcgtt ttcacgaagt
     2581 gacgtcatgg tatcaaaagt acgaacgcgc tcgcaagagg ttttgcgcgc gctccacagc
     2641 gtctccacaa gaattttatt ttactcacag gttagggact tagatcagcc ggctgccgac
     2701 gaaaatcagc gtgaccgcaa cggccggccg cagccagcgt gcgtgcaagc gagtgcacag
     2761 cttacttccg atgtatacgc cgggcagcga gccgatcagt aagttgacgg ccagcccaac
     2821 gttgacgtca cccatcgtcc agcgcccgag cgccgcggcc ggaatcaaga acgctgcgaa
     2881 cgcgatgtcg gatccgatca gctccgggag cccgacgagc ggcagcgcga gcgcgagcag
     2941 cggcagcgtc acggctccgc tgccgatcga cgtcagcgtc acgatgaatc cgacgacgac
     3001 gccgagcgcg atgacgcgcg cccgctgacc cggcttccag ctgaaaggct ggccgagcga
     3061 cgccacgccg tggcgcttga gcagcggctg cgccagaatc atgccggccg cgagaaagag
     3121 cgtgatgccg atcgcgcggc gcgtccacag ttcgaccaat gcgacgtcga tgtgatggcg
     3181 cagccagaag agcagcgcca agctcaggag cgacgccggc agcccgcccc acagcagcgc
     3241 tctgacgatc tcgccgttca cggtcccctg ctgacggtgc aggaacgccc cgtacagacg
     3301 cgtggggacg ctgtagagca ggtccgtccc gaccgccgtg agggggttga ccttcaggag
     3361 cgcgacgagc agcggggtca tgatgctccc gcccccgact cccgtcagcc cgacgagaac
     3421 gcccacgata agaccgatga gaatcagctc gagattgacg aagttcatct atttccgcaa
     3481 aatttgggga gcctcgtccg atgactatag ctaacttttg ggggaatgcc accccccgtt
     3541 cgtgacgagc ccccgggctc ccccgacgcc ccgatttttc cgatcggctc agcggaaacg
     3601 ggcgctccga gcgcgcgtac ccggccgtga ttcggctatg gattcgcggt tggggcacgc
     3661 cctccgctcc ggaaaaccgc gacttcggtc ggcacgacga agacctcctc gcctttggtc
     3721 agcgagagtt cccggaaccg gtcctgcgac atttcaacct gtaccggcgc gccccactcg
     3781 gtcaccgcct cgaccttcac tcgcggcccg gcggcgttga tgtgccgggt ggtcgcgcga
     3841 aagtgatcga ctcccgccgg gagccggcgc gtgatctcga gtgagtgcgg ccggacgaag
     3901 acgagatggt cggtcgcggt ccgatcgatg taggccgtgc cgttgtcgat ccggccgtgg
     3961 aagagattca cgctgccgag aaagttgaac acgaacgagg tcgccggccg gttgtagact
     4021 tcctccggcg tgccgatttg ctcgatccgc ccttcgttca tcaccgcgac ccgatccgct
     4081 acttccagcg cctcttcttg atcgtgcgtc acgatcacgg tcgtcgtgtg cgtctcgtcg
     4141 tgcaggcgcc gcagccacgc gcgcagctcc gtgcgcaccc gtgcatcgag cgcgccgaac
     4201 ggctcgtcga gcagcaacac gctcggttcg accgcgaggg cgcgcgcgag cgcaacgcgc
     4261 tggcgttgac ctcccgagag ctgcgacgga tagcggccgg cctgattccg cagctgaatg
     4321 agatccagca ggtcgtggac cttggcacgg atttgcgctt cgctgggtc
//
LOCUS       DASUCJ010000403         1362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_523977, whole genome shotgun sequence.
ACCESSION   DASUCJ010000403 DASUCJ010000000
VERSION     DASUCJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523977"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFU90_04805"
     CDS             complement(<1..560)
                     /locus_tag="VFU90_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5479128.1"
                     /translation="MLTALVQRGNEFVAKASLLDAAWPHAVVEEGNLAMQISAIRRVL
                     AEAGGARWIETLHRRGYRFVGPVKVIDDNSATSMSQRSNLPAALTSFIGREGELAELK
                     RLLSTKRLLTIVGAGGIGKTRVALQAAAEVVDAYPDGVWLTELAPIRDPLLVAATVAR
                     ALGIEERTRTPAIESLRGYLKSRQALL"
     gene            789..1082
                     /locus_tag="VFU90_04810"
     CDS             789..1082
                     /locus_tag="VFU90_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479129.1"
                     /translation="MSPILGQFLRLSARREVAIYLLEGRLWVADFVDGRGALFEPETW
                     FRFNCASPGARAARRRMALESGLPLSAEIVAKIDELHRAASETGVASPGNDAA"
     gene            1079..>1362
                     /locus_tag="VFU90_04815"
     CDS             1079..>1362
                     /locus_tag="VFU90_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479130.1"
                     /translation="MKSYDEVRRSPSLLREDSFTRGILMNTRPSIATLATRLCTAVLA
                     VCTTIGFASLLARRDAAAATIFVTTTQQKISDSGGCSLQEAIWASRLQLSA"
BASE COUNT          233 a          451 c          444 g          234 t
ORIGIN      
        1 agcagcgcct gccgcgactt gagatacccg cgcagcgact cgatcgccgg ggtacgcgtt
       61 ctctcctcga tgccgagcgc gcgcgcaacg gtggcggcaa caagcaaggg gtcgcgaatc
      121 ggtgccagct cggtgagcca gacgccgtca gggtaggcat cgacgacttc ggctgccgcc
      181 tgcagtgcga cgcgggtctt gccgatgccg ccggctccga caatcgtcag caggcgcttg
      241 gtcgatagca gccgcttaag ctccgccagc tcgccttcgc ggccaatgaa cgaagtgagt
      301 gcggccggta gattcgaccg ctgcgacatc gatgtcgcgg agttgtcatc gatcaccttg
      361 accggcccga cgaagcgata accgcggcgg tgtaacgttt cgatccatcg cgcaccgccc
      421 gcttcggcga gcacccggcg gatcgcagag atctgcatcg cgaggttgcc ttcctcgacg
      481 acggcgtggg gccacgccgc atcgagcagc gatgccttgg caacgaattc attgccccgc
      541 tgcaccagcg cggtgagcac agcgactccg cggacaccca gcctcgtcgg gcgtccgtcg
      601 cacgtgagcg cgccgacgtc cgggtccagc cggaagctgc cgatctcgaa gatctgggac
      661 atgcccttcc tcggtcaacg gcagttttat cgattcttca ggatttccac aagactcggg
      721 cgtccgacgc gggcgacgat tgaggcccgg atcgccgacg tagcgtacaa gccgacggaa
      781 gtccactggt gagcccgatc ctcggacagt tcctgcggct ctcggcgcgg cgcgaggtcg
      841 cgatctacct gctcgaaggg aggctatggg tggcggattt cgtcgacggc cgcggcgcgc
      901 tcttcgagcc cgagacgtgg ttccgcttca actgcgcgtc gcccggtgcc cgtgcggcga
      961 gacgaaggat ggcgctggaa tcgggcttgc cgctgtcggc tgaaatcgtc gcgaagatcg
     1021 acgagcttca tcgagcggcg agcgagacgg gcgtcgcaag tccagggaac gacgccgcgt
     1081 gaagtcgtac gacgaagttc gacgatcgcc gtcgttgctg cgtgaagact cgttcacccg
     1141 agggattctc atgaacacca ggccatcgat cgccacgctc gccacccgac tttgcaccgc
     1201 ggttctcgcc gtttgcacga ccatcggctt cgcttcgctc cttgcacgcc gcgacgcggc
     1261 tgccgccacc atcttcgtca cgacgaccca gcagaagatc agcgacagcg gcggttgttc
     1321 gctgcaagag gcgatctggg cgtcgcgtct tcagctgagc gc
//
LOCUS       DASUCJ010000404         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1395139, whole genome shotgun sequence.
ACCESSION   DASUCJ010000404 DASUCJ010000000
VERSION     DASUCJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395139"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFU90_04820"
     CDS             <1..312
                     /locus_tag="VFU90_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479131.1"
                     /translation="FAAGEFDADTAAGELATALRAEQLVFLTDVDGVLDGERRVMPAL
                     SVAQTQALIAEGVAAGGMIPKLEAAVRAAGAGCRTRIVNGTHAHALASALAGEGGTAV
                     V"
     gene            complement(529..1140)
                     /locus_tag="VFU90_04825"
     CDS             complement(529..1140)
                     /locus_tag="VFU90_04825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5479132.1"
                     /translation="MDVRETDADDLAVTLSDGIVTLRSWSRDFAEFMAEASADPAIQR
                     YNSPLDRLGRPPPPLSIMDATAIIDEFALKWRAFSTSGTPSGVAFTIMNAASGELVGC
                     WGVDDWSKADVAQIGYWLAPSARRRGFATRAVILLTRWLFELGAARGFLTIVAGNEAS
                     IAVAQRAGFVYEGTMRSHGVWQGRRCDVMWFAALPVEWVRKTD"
BASE COUNT          203 a          402 c          376 g          202 t
ORIGIN      
        1 ttcgcggccg gcgagttcga cgccgacacc gccgcgggcg agctggcgac ggcgctacga
       61 gcggagcaac tcgtgttcct gacggacgtc gacggcgtgc tcgacggcga gcgtcgcgtc
      121 atgccggcgc tttccgtcgc ccaaacgcag gcgctgattg cggaaggcgt cgccgccggc
      181 ggcatgatcc cgaagctcga agccgcggtg cgcgctgcgg gcgccggctg ccgcacgcgc
      241 atcgtcaacg gcacgcacgc ccacgcgctc gcgtccgcgc tcgccggcga gggcgggacg
      301 gcggtcgttt agacgcttca cggcacgatc acaccaggtt caaattcgga acagacgtct
      361 cggagtggcc atcgagcgct tcgaccgctc ggctggggcc aaatggtccc tggccgtggc
      421 taggcgtcga accgatgatc gttaggacag cactccgtcg tcgcggtcgg attcgcgctc
      481 ggtgacattt ctggtcggcc ctgttggctc cggatcgcgt cacgtctgtt aatctgtctt
      541 gcgcacccat tcaaccggca gcgcagcgaa ccacatcacg tcacagcgcc ggccctgcca
      601 cacgccgtgt gaacgcatcg ttccctcgta cacaaacccc gctcgttgag cgaccgcgat
      661 tgacgcttca ttcccggcga cgatcgtcaa gaagccacgc gcggcgccaa gctcgaacaa
      721 ccagcgtgtg agcagaatca ccgctcgagt cgcgaagccg cgtcgtctcg cacttggtgc
      781 gagccagtaa ccgatctgtg ctacgtccgc tttcgaccag tcgtctaccc cccagcaccc
      841 gaccaattcg ccggacgccg cgttcatgat cgtgaacgcg acaccagagg gtgtcccgct
      901 cgtggagaat gcccgccact tcagcgcgaa ttcgtcgatg atcgccgtag cgtccatgat
      961 cgagagcggc ggaggcgggc gcccgagtcg gtcgaggggc gagttatagc gttgaatcgc
     1021 ggggtcggca ctcgcttcgg ccatgaactc ggcgaagtct cgtgaccacg accggagcgt
     1081 gactatgccg tcggaaagcg tcacagcaag atcgtcggcg tccgtctctc gcacgtccac
     1141 tccggaatcc ttgaccggta actccaggtt cgaggcgtga tcg
//
LOCUS       DASUCJ010000405         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_447603, whole genome shotgun sequence.
ACCESSION   DASUCJ010000405 DASUCJ010000000
VERSION     DASUCJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447603"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFU90_04830"
     CDS             <1..365
                     /locus_tag="VFU90_04830"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEU5479133.1"
                     /translation="APGAAFCAFVPRLFDYHPLAVPVPYNHSSVDCDEVLYYVSGNFM
                     SRRGVEEGSITLHAAGAPHGPQPGAVEASLGKTSTDEIAVMVDTFKPLRIGEWASSCE
                     DGDYYRSWLENPASAASS"
     gene            complement(362..>1049)
                     /locus_tag="VFU90_04835"
     CDS             complement(362..>1049)
                     /locus_tag="VFU90_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5479134.1"
                     /translation="LDVELEVGFFTGRPNALGVPIPAGDAYEHIAGIVLVNDWSARDI
                     QAWEYQPLGPFLGKSFATTISPWVVTLDALEPFRCAAPVQDPVPLPYLAEPVRRGYDV
                     ALTIELQSARMRDRGIVPQIVSRTNFRAMYWTMAQQLAHATANGAATQAGDLFASGTI
                     SGSQPDSYGSFIELTWRGERPLELPGGERRAFLEDGDEVTLRGRCERAGARGIGFGTA
                     RGRIVPARPA"
BASE COUNT          173 a          372 c          341 g          163 t
ORIGIN      
        1 aagcgcccgg cgccgcattc tgcgcgttcg tgccgcgcct tttcgactat catccactcg
       61 ccgtgcccgt tccgtacaat cactccagcg tcgactgcga cgaagtgctg tactatgtca
      121 gcggcaattt catgagccgc cggggcgtcg aggaaggttc gatcacgctg catgcggcgg
      181 gcgcgccgca cggaccgcag ccgggcgccg tggaggccag cctcggcaag acgtcgaccg
      241 acgagatcgc cgtaatggtc gatacgttca agccgctgag gatcggcgag tgggcgagca
      301 gctgcgaaga cggcgactac tatcgctcct ggctcgaaaa ccccgcctcc gccgcgtcct
      361 cctaagccgg gcgcgccgga acgatccgcc cgcgcgccgt tccgaagccg atgccgcgcg
      421 cacccgcccg ctcgcaccgg ccgcgcagcg ttacctcgtc gccgtcttcc aggaacgcgc
      481 gccgctcgcc gccgggaagc tccagcggcc gttcgccgcg ccacgtcagc tcgatgaaac
      541 ttccgtagct atcgggctgc gaccccgaaa tcgttccgga tgcgaataga tcgccggcct
      601 gggtcgcggc cccgttggcg gtcgcgtgcg cgagctgctg cgccatcgtc cagtacatcg
      661 cgcgaaagtt cgtgcgcgag acgatttgcg gcacgatacc ccggtcgcgc atccgcgcgc
      721 tttgcagctc gatcgtcagc gcgacgtcgt agccgcggcg gacgggctcc gcgagatacg
      781 gcaacggcac cggatcttgc acgggcgccg cgcaacggaa cggctcgagc gcgtcgagcg
      841 tgacgaccca cggtgagatc gtggtggcaa acgatttacc caggaacggt ccgagcggct
      901 gatactccca ggcttgaatg tcgcgcgcgc tccagtcgtt gaccaggacg atccccgcaa
      961 tgtgttcgta cgcgtcgcca gccggaatcg ggacgccgag cgcgttcggc cgcccggtga
     1021 agaaaccgac ctccagctcg acgtcgagc
//
LOCUS       DASUCJ010000406         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1119975, whole genome shotgun sequence.
ACCESSION   DASUCJ010000406 DASUCJ010000000
VERSION     DASUCJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119975"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VFU90_04840"
     CDS             complement(<1..1019)
                     /locus_tag="VFU90_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479135.1"
                     /translation="MKIRKVSNAIVSVALGAILASCGGRTGAVVPPLAPASNAAAYAL
                     ARDVLPTPIRHVVIIFQENRTPDYMFQDVPGADVVKSAVDSYGHHVTLQPVSLAAPWG
                     LAHSRNAFLRDYDGGKMDGFNAKLPLSERLRPYGFGIPSEIRPYRDMAVQYVAADHMF
                     QSNRGPSYPAHLYIVSGTATEPKIHDDRISSNPFDGKTHQPRGGGCDTPSNTRVYTVD
                     LRTGSGGPAVYPCVDRPVLSDFLDARHISWRYYQNGLGPGLWHGFNSIWHVRFGRGFK
                     NVISPPQTVLADVAAGRLAGMSWVMPDAEHSDHADNRSAAGPSWVAAVVNAIGKSKYW
                     NSTAI"
     gene            complement(1006..>1670)
                     /locus_tag="VFU90_04845"
     CDS             complement(1006..>1670)
                     /locus_tag="VFU90_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479136.1"
                     /translation="TCFERPVLSDFLDRQHVAWRYYQQNRGPGLWHGFNSIRHVRFGP
                     DYANVISPPQTILSDVAAGRLPGVAWVMPDGPHSDHAANQSAEGPSWVAAVVNAIGKS
                     KYWSSTAIFVTWDDWGGWYDHVKPPIYNAYELGFRVPLIVVSPYAKRGYVSKVQHEFG
                     SILAFTEETFGIAKGALNATDKRADDLRDAFNFAQKPRAFTTIKAPPFKPGVLGVDDE
                     DP"
BASE COUNT          304 a          526 c          532 g          308 t
ORIGIN      
        1 aagatcgccg tgctgttcca gtatttgctc tttccgattg cgttgacgac ggcggctacc
       61 cacgagggtc ctgcagcact gcggttgtcg gcgtggtcgg agtgctccgc gtccggcatc
      121 acccatgaca tccccgccaa ccgccccgcg gcaacgtcgg cgagcaccgt ctggggcggc
      181 gaaatgacgt tcttaaagcc gcgcccgaac cgcacgtgcc aaatcgaatt aaatccgtgc
      241 cacagtcccg ggccgagtcc gttttggtag tagcgccacg aaatgtgccg cgcgtccaaa
      301 aagtccgaga gcaccggacg atcgacgcac gggtacaccg ccggtcctcc cgaaccggtg
      361 cgcaggtcta cggtgtacac gcgagtattg gagggcgtat cgcacccgcc gccccgcggt
      421 tggtgcgttt tcccgtcgaa cggattactc gaaattcgat cgtcgtggat cttcggctcc
      481 gtagcggttc cgctcacgat gtacaggtgg gcgggatagc tcggtccgcg gttgctctga
      541 aacatatggt cggcggcgac gtactgcacc gccatgtcgc gatacggccg aatctccgac
      601 gggatgccga agccgtacgg tcgcagccgt tcgctcaaag gaagcttcgc gttaaatcca
      661 tccattttgc cgccgtcgta atcccgcagg aaggcgttgc gcgagtgcgc gagcccccac
      721 ggggcggcca gcgacaccgg ctgcaaagta acgtggtggc cgtacgagtc gacggcgctc
      781 ttgaccacgt ccgcccccgg cacgtcttga aacatgtaat cgggcgtgcg gttttcctga
      841 aagatgatga cgacgtgccg aatcggcgtt ggcagtacgt cgcgcgccaa ggcataggcg
      901 gccgcgttcg acgccggcgc gagcggaggg acgacggctc cggtccgtcc gccgcacgac
      961 gccaagatgg cgccgagcgc cacggaaacg atcgcgttgg agactttacg gatcttcatc
     1021 gtcgacaccg agcactccag gtttgaacgg cggggctttg atcgtggtaa aggcgcgcgg
     1081 cttctgcgcg aaattgaacg cgtcccgaag gtcgtccgcc cgcttgtcgg tcgcgttcaa
     1141 cgcgcctttg gcgataccga atgtttcctc ggtgaaggcg aggatgctgc cgaattcatg
     1201 ctgcaccttc gatacgtatc ctcgcttcgc atagggcgat acgacgatca gcggcacgcg
     1261 gaagccgagc tcgtacgcgt tgtagatcgg cggctttacg tgatcgtacc agccgcccca
     1321 gtcgtcccag gtcacgaaga tcgcggtgct gctccagtac ttgctcttgc cgatcgcatt
     1381 gacgactgcg gccacccacg acgggccttc agcgctctga ttggcggcat ggtccgagtg
     1441 cggaccgtcc ggcatcaccc acgcgacgcc cggcagccgt cccgccgcga cgtccgacaa
     1501 aatcgtctga ggcggcgaga tgacgttggc gtagtcgggg ccgaagcgga cgtggcgaat
     1561 cgagttgaat ccgtgccata accccggtcc gcgattctgc tggtaatacc gccacgcaac
     1621 gtgctgccgg tcgagaaagt cggagaggac cgggcgttcg aagcacgtga
//
LOCUS       DASUCJ010000407         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1318699, whole genome shotgun sequence.
ACCESSION   DASUCJ010000407 DASUCJ010000000
VERSION     DASUCJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318699"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..554
                     /locus_tag="VFU90_04850"
     CDS             402..554
                     /locus_tag="VFU90_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479137.1"
                     /translation="MTIRRGSSSQTLTARTTPDVAPGSRESVIAGLFRRLLNDLQDRM
                     RPYMVI"
     gene            complement(683..1204)
                     /locus_tag="VFU90_04855"
     CDS             complement(683..1204)
                     /locus_tag="VFU90_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5479138.1"
                     /translation="MDQIPAIAEVFAYLSLLTIGGGMAAYPEMKTMTIDVHHWLTNAQ
                     LTHIYSVGQMAPGPNMMMVSAIGVRVAGIAGAAVAALAFFIPTGILTFAVGRIWTKLA
                     DWPWRNSIQLGLAPVAIGLAVAGTISFGKTAVTGWVTVLVALAVFYATMRTKVNPALL
                     ILGGAIVGGIALR"
     gene            complement(1183..1842)
                     /locus_tag="VFU90_04860"
     CDS             complement(1183..1842)
                     /locus_tag="VFU90_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5479139.1"
                     /translation="MSTSEQPPGEDSAAAATPATPPAAMATRPPPSVLDVFLTFLLIG
                     ATSFGGGVVAYLRAALVRKKAWMDDKEFLELMSISNTLPGLNATNMSILAGDRMRGTV
                     GAIAGLLGMCIPGFIFMTIAGILYSHEGDRPIVTAALKGVAAAATGLIAATWYQIGKK
                     SVRGFYDAFFILAAVLGVNHFKLSVPIVLLAVGALSIFANRPQKEHRKEPLAEWIRSL
                     R"
     gene            1963..>2773
                     /locus_tag="VFU90_04865"
     CDS             1963..>2773
                     /locus_tag="VFU90_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5479140.1"
                     /translation="MKRLALLGCGLALSGCAALLNAGRPAYNGPVLTPLWHVADLEVV
                     DTPLIGDGRVYTAARAGQSQPPRAYAFDAQTGGRLWVSSAPASGLLALAGGTVLAADV
                     RGTAHAVDARDGRTIPAFAPRPVAFAVAQDDAFYLTNGAQLAALRSMGQTRWTHALPI
                     ERAVPVVAGETVYAFGRHHVDEMTPDLIGVYAFATQSGAARWKREWRQSFGKYRYDPK
                     LRAMTWKVKPDYTTIAAVVADDRTAYVFEQLRDERLLATAQRIVAVDARTGT"
BASE COUNT          500 a          975 c          834 g          464 t
ORIGIN      
        1 acgtcacgtc ggcgctccgg tgcgagttac gtacgataat gaacgcatta ccggcgaaaa
       61 tattcgcaac tctctgcctc gtcttcaccg gtacattcgg gcgcaccgaa gccggcgacg
      121 acctgcggta cgccgccata acgcgaattg aactacgtgg atcgatcgta gccgggtgcg
      181 taatcgaagg ctgggcgacg ccggacaagg tttcgctcaa cgaagccgtc tgcgtacgcc
      241 gcggatcatc gtattccgtc ggtgggtacg ccagctttat gcccaaaagg tcgccgggcg
      301 agatattcgc cgtactggtg gccgatatca agtccgcgcg cgctctggat gtagcgcagc
      361 cacccttacc gcaaggctcc ccaaacgcgc ccaccggcag cgtgaccatt cggcgtggca
      421 gcagcagcca aacgctgacg gctcgcacga cgcccgacgt cgcgccggga agtcgagaat
      481 cggtgatcgc cgggctcttc cgccgcctgc tcaacgacct gcaggatcgc atgcggccat
      541 acatggtcat atgaccttga ccccaaaaaa ggaatacggc cgccggtgcg ggcctcggcg
      601 accgtatagc tttcggtatt ggataccggt ccgtatgata ccgcaaaacg gcccttacag
      661 tccatcacca attttggcta ggtcagcgca aggcgatgcc gcccacgatg gcgccgccca
      721 ggatcagcag cgccggattg accttcgtgc gcatcgttgc gtaaaacacc gccagcgcga
      781 ccagcacggt cacccagccg gtcaccgccg ttttcccgaa gctgatcgta ccggccacgg
      841 ccagcccgat cgccaccggg gccagcccca gctggatcga gttgcgccac ggccagtcgg
      901 ccagcttcgt ccagatgcgc ccgaccgcga acgtcaagat tccggtcgga atgaagaacg
      961 ctagcgccgc caccgccgcc ccggcgatgc cggcaacccg cacgccgatc gcagagacca
     1021 tcatcatgtt cggccccggc gccatctgcc cgaccgagta gatgtgggtc agctgcgcgt
     1081 tcgtcagcca gtgatgaacg tcgatcgtca tcgtcttcat ctcgggatac gcggccatac
     1141 cgccgccgat cgtcagcagc gagagatagg cgaagacttc ggctatcgca gggatctgat
     1201 ccattcggcc aagggctcct ttcgatgttc tttttgcgga cggttggcga agatcgacag
     1261 cgcgccgacg gccagcagca ctatcggaac ggacagcttg aagtggttta cacccagcac
     1321 cgccgccaag atgaagaacg cgtcgtaaaa gccgcgcacc gacttcttcc cgatttggta
     1381 ccacgtggcc gcgatcagtc cggtcgccgc cgccgccacg cctttgagcg ccgccgtcac
     1441 gatcggccgg tcgccttcgt ggctgtagag aattccggcg atcgtcatga agatgaagcc
     1501 gggaatgcac atgcccagca gcccggcgat cgcgccgacc gttccgcgca tccggtcgcc
     1561 ggccaagatc gacatgttcg tcgcgttgag ccccggcagc gtgttgctga tcgacatcag
     1621 ctcgagaaac tctttgtcgt ccatccacgc ttttttgcgc accagcgcgg cgcgcagata
     1681 ggccaccacg ccgccgccga aactggtcgc gccgatcagc agaaacgtta ggaagacgtc
     1741 gagcaccgac ggcggcggtc gcgtcgccat tgcggccggc ggcgttgccg gcgtcgcagc
     1801 agcggcagaa tcttcgccgg ggggctgctc cgatgttgac atacgcgtcg aaacctccga
     1861 aagcacctac gcgcccctca tcttaatccc aatcgcattt cgacctcccg cttcgcgcca
     1921 aaaaggtcgc tcccgcgcgc ttcgcgtatc gctcgcagca cggtgaaacg cctcgcgctt
     1981 ctaggctgcg gtctcgcgct gtcgggctgc gccgcgttgc tcaatgcggg ccgtccggcc
     2041 tataacgggc cggtcctcac gccgttgtgg cacgtcgccg atctcgaggt cgtcgacacg
     2101 ccgctgatcg gcgacggccg cgtctacacg gcggcccgcg ccggccaatc gcagccgccg
     2161 cgtgcgtacg cgttcgacgc ccaaaccggc ggccggctgt gggtgtcctc cgcgccggct
     2221 tcgggtttgc tcgcacttgc cggcggcacc gttctcgccg ccgatgtccg cggcaccgcc
     2281 catgccgtcg acgcgcgcga cggccgcacc atccccgcgt tcgccccgcg tccggtcgcg
     2341 tttgccgtcg cacaagacga cgcgttctat ctcaccaacg gcgcgcagct cgcagcgctg
     2401 cgcagcatgg ggcaaacccg ctggacgcac gcactgccga tcgaacgcgc cgtcccggtc
     2461 gtcgccggcg agaccgtcta cgccttcggc cggcatcacg tcgacgaaat gacgcccgac
     2521 ttgatcggcg tctatgcgtt cgcaacgcag agcggcgcgg cgcgctggaa acgcgagtgg
     2581 cgccaaagct tcggcaagta tcgctacgat ccgaaactgc gcgccatgac gtggaaggtc
     2641 aaacccgact acaccacgat cgcggccgtc gtcgccgacg accgcaccgc ctacgttttc
     2701 gaacagctgc gcgacgagcg tctcttggcc accgcgcaac ggatcgtggc cgtcgacgcc
     2761 cgtaccggca cga
//
LOCUS       DASUCJ010000408         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_615747, whole genome shotgun sequence.
ACCESSION   DASUCJ010000408 DASUCJ010000000
VERSION     DASUCJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615747"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFU90_04870"
     CDS             <1..332
                     /locus_tag="VFU90_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479141.1"
                     /translation="LVSSIVGISGNAGQAAYAASKAGLIALAKSLAKELGSRNIRVNA
                     VAPGLIETAMTQKMPDTARELLIKQAALGRPGTPEDVSGAVAFLCSDAAGYITGQTLV
                     VDGGVLI"
     gene            376..624
                     /locus_tag="VFU90_04875"
     CDS             376..624
                     /locus_tag="VFU90_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5479142.1"
                     /translation="MPTTFDKVKKIIVEQLGVDESEVTPEASITDDLGADSLDQVELV
                     MAFETEFNIDIPDEEAEKIKTVGDAVKRVDEATAGSSA"
     gene            578..1963
                     /gene="ffh"
                     /locus_tag="VFU90_04880"
     CDS             578..1963
                     /gene="ffh"
                     /locus_tag="VFU90_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5479143.1"
                     /translation="MRSNASTRRPPGLRHNRNLFDQLSDRLTAVFARLSGRGRLSESD
                     VTEALGEVRVALLEADVSLGVAKSFVARVKEAAIGANVLESLTPAQTIVKIVYDELVA
                     LLGGAQARLQFSDAPPSVIVLVGLQGSGKTTQAGKLALRLKEQGRRSLLIAADVARPA
                     AIAQLQTLGKQVDLPVFERGSGDPVAIARDGVAEARRLGISTAIVDTAGRLQIDEQLM
                     DELGRIVEAVRPKEILFVADAMTGQEATNVAKGFHDRLGITGVILTKMDGDTRGGAAL
                     SIYSETGAPIKLVGVGEKLSALEPFYPERLASRILGMGDVLTLIEKTQNLYSAEQTKQ
                     LEEKLLKHSFTLEDFLDQMRQVRKLGSMTDILKMVPGLSNRLPKDFEIPEKELGKIEA
                     IICSMTRRERRNPEMLNGSRRKRIALGSGTQVSDVNRLVKQFEQSRQMMKQFGGGKKR
                     RMPRLPAGLFR"
     gene            1988..>2172
                     /gene="rpsP"
                     /locus_tag="VFU90_04885"
     CDS             1988..>2172
                     /gene="rpsP"
                     /locus_tag="VFU90_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5479144.1"
                     /translation="MVRIRLRRMGAKKQPTYRFVVADARAPRDGRFIEILGHYNPRTE
                     PRTIVVDEGKAKEWLSKG"
BASE COUNT          447 a          653 c          710 g          362 t
ORIGIN      
        1 tcctggtctc gagcatcgtg ggcatctcgg gaaacgcggg gcaagccgcc tacgccgcat
       61 cgaaagcggg cctgatcgcg ctcgccaaat cgctggcgaa ggagttgggt tcgaggaata
      121 taagagtcaa cgccgtcgcg ccgggtctca tcgagacggc catgacgcag aaaatgcccg
      181 acacggcccg ggagctctta atcaagcaag ccgccctggg gcggcccggg acgcccgagg
      241 acgtgagcgg cgcggtggct tttctctgtt ccgacgccgc cggctacatc accgggcaaa
      301 ccctggtcgt cgacggcggc gtgttaatct gagttcggat ctcgatttcg tacccgtcgt
      361 caataaggag acgtaatgcc aaccaccttc gacaaagtga agaagatcat cgtcgagcag
      421 ctcggcgtcg acgagtcgga agtgactccc gaggcctcga tcaccgacga tcttggtgcc
      481 gattcgctcg accaggtgga gctggtgatg gcgttcgaga ccgagttcaa catcgacatc
      541 cccgatgagg aagccgagaa gatcaaaacg gtaggcgatg cggtcaaacg cgtcgacgag
      601 gcgaccgccg ggtcttcggc ataaccgtaa tttgttcgat caactcagcg accgtctgac
      661 ggccgttttc gctcggttga gcgggcgcgg ccggcttagc gaaagcgacg tcaccgaagc
      721 attgggcgaa gtccgcgtcg ccctgctgga ggccgacgtt tcgttgggcg tcgcgaagtc
      781 gttcgtcgct cgagtcaaag aggcggcgat cggtgcaaac gttctcgaat cgctgacgcc
      841 cgcccagacg atcgtcaaga tcgtctacga cgaactcgtc gcgctcctgg gcggggcgca
      901 ggcgcgcctg cagttctccg acgcgccgcc gtcggtgatc gtcttggtcg ggctgcaggg
      961 ctccggaaag acgacgcagg ccggtaaact cgcgctgcgg ctcaaagaac agggccggcg
     1021 ctcgctgcta atcgccgccg acgtcgctcg cccggcggcc atcgcgcagc tgcagacgct
     1081 cggaaagcag gtcgacttgc cggtgttcga acgcggcagc ggcgacccgg tcgcgatcgc
     1141 gcgcgacggc gtagcggaag cccgccggct gggaatctcg acggcgatcg tcgataccgc
     1201 cggacgcctg cagatcgacg agcagctcat ggacgagctc gggcggatcg tcgaggcggt
     1261 gcgcccgaaa gagattctgt tcgtcgccga tgcgatgacc gggcaagagg cgaccaacgt
     1321 cgccaagggt tttcacgatc ggcttgggat caccggcgtc atcctcacca agatggacgg
     1381 cgacacccgc ggcggtgcgg cgctctcgat ttactccgag acgggcgcgc cgatcaagct
     1441 ggtcggcgtc ggcgagaagc tctcggcctt ggagccgttt taccccgagc ggctcgcgtc
     1501 gcggattctc gggatgggcg acgtgcttac gctcatcgag aagacgcaaa atctctactc
     1561 cgcggagcag acgaagcagc tcgaggaaaa gcttctcaag cacagcttta cgctcgagga
     1621 cttcctcgat caaatgcgtc aggtgcgcaa gctcggctcg atgaccgaca tcttgaaaat
     1681 ggtacccggg ctgtcgaacc ggttgccgaa ggatttcgaa attccggaaa aagagctcgg
     1741 caagatcgaa gcgatcattt gctcgatgac gcggcgcgag cgccgcaatc cggagatgct
     1801 caacggctcg cggcgcaagc ggatcgcgct gggatcgggc acccaagttt ccgacgtgaa
     1861 ccggctcgtc aaacagttcg agcagtcgcg tcagatgatg aaacagttcg gcggaggcaa
     1921 gaagcgtcgg atgcctcggc ttccggcggg actgtttcga taaatactac ccacacgaaa
     1981 gcagaacatg gtaagaatta gactgcgccg gatgggcgcc aagaaacagc cgacgtaccg
     2041 tttcgtcgtc gcggacgcgc gagcgccgcg cgacgggcgg ttcatcgaaa tcctgggaca
     2101 ctacaatccc cgcaccgaac cccgcacgat cgtcgtggac gaaggcaaag cgaaagagtg
     2161 gctgtccaag gg
//
LOCUS       DASUCJ010000409         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_218379, whole genome shotgun sequence.
ACCESSION   DASUCJ010000409 DASUCJ010000000
VERSION     DASUCJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218379"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFU90_04890"
     CDS             complement(<1..747)
                     /locus_tag="VFU90_04890"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEU5479145.1"
                     /translation="MSDALEFGRAVCGDLAAAERREWLAVNGLGGFASGTIAGSLTRR
                     YHGLLIAALRPPGQRTLVLTKIDETVRYRGTPYALATNRWRDGYVAPQGFTTIERFYL
                     DGTTPVWEFALADALLEKRVWLEPGANTVYVRYRCLRAGGPLDLSLLALANYRDFHGN
                     THAGNRPAAVTPAGARSLRVEAGPRPMWIAADAGTVAVENVWYRDFVLAQETRRGLDD
                     RDDNLAAGTFTATLAPLESVTLAASTDADPS"
     gene            complement(744..>3407)
                     /locus_tag="VFU90_04895"
     CDS             complement(744..>3407)
                     /locus_tag="VFU90_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479146.1"
                     /translation="LQQDKRGVEAWRKWGPYLSERQWGTVREDYSPDGTAWDYFPHDH
                     ARSRAYRWGEDGIGGICDEHQLLCFAVACWNGRDPILKERLFGLTNREGNHGEDVKEY
                     YFYLDNLPSHAYMKMLYRYPHAAYPYARLVEENARRTKTDPEFELIDTGAFDEDRFFD
                     VTIEYAKNAPEDIFVRLTAVNHGPEAATLHLLPTIWFRNTWSWRHGAFKPNLRREESH
                     AAGVDLVVAEHERFGEMRLYCDAGELLFTENETNFARLYGVSNAGPFVKDGIGEYVVG
                     GRSDAVNPQHAGTKAAIHYELTLQPHESASVRLRLTGRSDLADPFAGLSNVVEKRRIE
                     ADEFYAGVAGVPLDDDVRAIQRQAFAGILWSKQFYYYVVQEWLDGDPAMPPPPAQRRR
                     GRNCGWEHLHAADVLSMPDTWEYPWFAEWDLAFHCVVLALLDPEYAKKQMDLLTREWY
                     LHPNGELPAYEWAFGDVNPPLWAWAARRVYQIEAKMTGSGDRLFLERVFQKLLLCFTW
                     WVNRKDADDNNVFQGGFLGLDNIGVLDRDAALPPGAHIDQSDGTAWMAIFSGNMLAIA
                     LELAAGQPAYEDIASKFFAHFLYIAKAINEIGADGLWDNADGFYYDYLHVDGGEPIPL
                     RVRSLVGLIPLIASEVAEGDVLEKLPDFAKHVGWFVRHRPDLTESIAYMERGGIDERY
                     LLALADDDKLIRLLDRMLAPNEFLSAYGIRSLSRVHLEHPYVLSLDGTEHRIGYEPGE
                     SVTGTFGGNSNWRGPIWFPINFLLIEALQKLHYYYGDNFKVQMPAGSGYYSTLWDVAT
                     DLSQRLISIFERDASGRRPVFGNNETFQTNPLWRDLIPFYEYFHGDDGTGLGAAHQTG
                     WTALVGKLILQCGEYAGQDKHPLTGFRWKTP"
BASE COUNT          581 a         1057 c         1175 g          594 t
ORIGIN      
        1 ggacgggtcg gcgtcggtgg aggcggcgag cgtcaccgat tcgagcgggg ccagcgtcgc
       61 ggtgaaggtt ccggcggcga gattgtcgtc gcgatcgtcc agcccgcgcc gcgtttcttg
      121 cgcgagcacg aaatcgcgat accagacgtt ctcgaccgcg acggtgccgg cgtcggcggc
      181 gatccacatc ggccgaggac cggcctcgac gcgcagcgag cgcgcgccgg ccggcgttac
      241 ggcggccggc cggtttccgg cgtgcgtgtt gccgtgaaaa tcgcggtagt tggcgagcgc
      301 gagcagcgac agatcgagcg gaccgccggc gcgcagacag cggtaacgca cgtagacggt
      361 attggcgccg ggctccagcc agacgcgttt ttcgagcagc gcgtcggcca gcgcgaactc
      421 ccaaaccggc gtcgtgccgt cgagatagaa gcgctcgatc gtggtgaagc cctgcggcgc
      481 gacgtagccg tcgcgccagc ggttggtcgc aagcgcgtag ggagtgccgc gatagcgcac
      541 cgtttcgtcg attttcgtca gcacgagcgt tcgttggccg ggaggccgga gggccgcaat
      601 cagcagaccg tgatagcggc gcgtgagcga gcccgcgatc gtccccgacg cgaaaccgcc
      661 tagcccgttg acggccagcc actcgcggcg ttcggccgcg gccagatcgc cgcagaccgc
      721 gcggccgaac tcgagcgcat cgctcacggg gttttccagc ggaatcccgt cagcggatgc
      781 ttatcctgac cggcgtactc cccgcattgc aggatcagct tgccgaccag cgcggtccag
      841 ccggtttgat gcgcggcgcc gagcccggtg ccgtcgtcgc cgtgaaagta ctcgtaaaac
      901 ggaatgaggt cgcgccatag cggattggtt tgaaaggttt cgttgttgcc gaacaccggc
      961 cggcgcccgg aggcgtcccg ttcgaaaatc gagatcaagc gctgcgacaa gtcggtcgcg
     1021 acgtcccaca aggtcgaata atagccggag ccggccggca tttgaacctt gaagttgtcg
     1081 ccgtaatagt agtgcagctt ctgcagcgct tcgatcagca agaagttgat cggaaaccaa
     1141 atcggaccgc gccagttgga attcccgccg aaggttccgg tgaccgactc gccgggctcg
     1201 taaccgatgc gatgttcggt gccgtcgagc gagagcacgt agggatgctc gaggtgcacg
     1261 cgcgacagcg agcggatgcc gtacgcgctg aggaattcgt tgggtgccag catccgatcg
     1321 agcagccgga taagtttgtc gtcgtcggcc agtgcgagca ggtagcgctc gtcgataccg
     1381 ccgcgctcca tgtaggcgat cgattcggtg agatcggggc ggtggcggac gaaccagccg
     1441 acgtgcttgg cgaaatcggg gagcttttcg aggacgtcgc cctcggccac ctcgctcgcg
     1501 atcagcggaa tcaacccgac cagcgagcgc acccgcagcg gaatcggctc gccgccgtcg
     1561 acgtggaggt aatcgtagta gaagccgtcg gcgttatccc acaggccgtc ggcgccgatt
     1621 tcgttgatcg ccttggcgat gtagaggaag tgcgcaaaaa acttcgaggc gatgtcttcg
     1681 taggcgggct gaccggccgc cagctcgagc gcgatcgcga gcatgttgcc gctgaagatc
     1741 gccatccagg cggtgccgtc ggactgatcg atgtgcgcac ccggcggcag cgcggcgtcg
     1801 cggtcgagca cgccgatgtt gtcgagcccg agaaaccccc cttgaaaaac gttgttgtcg
     1861 tcggcgtctt tgcgattgac ccaccaggtg aagcagagca gcagcttctg aaagacgcgc
     1921 tcgaggaaga gccggtcgcc gcttccggtc atcttcgctt cgatctggta aacgcgccgc
     1981 gcggcccacg cccacagcgg cgggttgacg tcgccgaagg cccattcgta agcgggcagc
     2041 tcgccgttgg ggtgcaagta ccactcgcgc gtgagcaagt ccatctgttt cttggcgtac
     2101 tcggggtcga gcagcgccag cacgacgcag tggaacgcga gatcccactc ggcgaaccac
     2161 gggtattccc acgtgtcggg catcgaaaga acgtcggcgg cgtggaggtg ctcccacccg
     2221 cagttgcgtc cgcggcggcg ctgtgcgggc ggcggcggca tcgcggggtc gccgtcgagc
     2281 cactcctgca cgacgtaata gtagaactgt ttgctccaca agatgccggc gaacgcctgc
     2341 cgctggatcg cgcgcacgtc gtcgtcgagc ggaacgccgg cgacgccggc gtaaaactcg
     2401 tcggcttcga tccggcgttt ctcgacgacg ttggacaggc cggcaaacgg atcggcgagg
     2461 tcgctgcggc cggtcagcct gagacggacc gacgcggatt cgtggggctg caacgtcagc
     2521 tcgtagtgga tcgccgcttt ggtaccggcg tgttgcggat tgacggcgtc gctgcgcccg
     2581 ccgacgacgt actcgccgat cccgtctttg acgaacgggc cggcgttgga cacgccgtag
     2641 aggcgcgcga agttcgtttc gttttcggta aacaggagct cgccggcatc gcagtacagg
     2701 cgcatttcgc cgaagcgttc gtgttcggcc acgacgagat ccacgccggc ggcgtggctt
     2761 tcctcgcgcc ggaggttcgg cttgaacgcc ccgtggcgcc acgaccaggt gttgcgaaac
     2821 cagatcgtcg ggagcagatg gagcgtcgcg gcttccggcc cgtgattgac ggccgtgagc
     2881 cgcacgaaga tatcctccgg cgcattcttc gcatattcga tcgtgacgtc gaagaagcgg
     2941 tcctcgtcga acgcgccggt gtcgatcagc tcgaattcgg gatccgtctt ggtgcggcgc
     3001 gcgttttctt cgacaagtcg cgcgtacgga taggccgcgt gcgggtagcg atagagcatc
     3061 ttcatgtacg cgtgcgacgg caggttgtcg aggtagaagt agtactcctt gacgtcctcg
     3121 ccgtgattgc cttcgcggtt ggtcaagccg aacagacgct ctttgagaat cggatcgcgg
     3181 ccgttccagc aggcgaccgc aaagcacagc agctggtgct cgtcgcagat gccgccgatg
     3241 ccgtcttcgc cccagcgata ggcgcgcgag cgcgcgtgat cgtgcgggaa gtaatcccag
     3301 gcggtgccgt ccgggctgta gtcctcgcga acggtgcccc attgccgctc gctgaggtac
     3361 gggccccact tgcgccacgc ctcgacgccg cgcttgtctt gctggag
//
LOCUS       DASUCJ010000410         1411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1135477, whole genome shotgun sequence.
ACCESSION   DASUCJ010000410 DASUCJ010000000
VERSION     DASUCJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFU90_04900"
     CDS             complement(<1..742)
                     /locus_tag="VFU90_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5479147.1"
                     /translation="MSRASIVGMLIAAEIVIVGIALYAIRGSANFANFHQVAFAAKPI
                     APIDAGSAPRITVDDPNSRIVVATSSDGQVHVSDLTSVSGSIFGDRWHVAQLQVQRTA
                     DGVSVSRPGSDDLHLHFIFGSFERRVEVDVPAASHVEIARCAGADVAGIEGGVAVRSQ
                     DGHVTLTDLRGTVDGRSDDGSVSATRVHGDSLALQSGDGHLSLQNVAVGSLNAQTSDG
                     SIEARDVTVSGTSPHAVLHSDDGSIRVDG"
     gene            1033..>1411
                     /locus_tag="VFU90_04905"
     CDS             1033..>1411
                     /locus_tag="VFU90_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5479148.1"
                     /translation="MRVGPEDGDLVAMTLRGNPEAFATLVERYDRAVYHLAYRTLHDV
                     EDARDATQEAFFKAFRSLRTFKPGARFSTWIFAIAYHACCDRLNRRKRYSNEELPDRA
                     DAGPGPEQQAIALDEAARLRTAID"
BASE COUNT          235 a          449 c          498 g          229 t
ORIGIN      
        1 atccatcgac gcgaatcgaa ccgtcgtcgc tatgcagcac ggcgtgcggc gacgtaccgc
       61 tgacggtgac gtcgcgggcc tcgatgctgc cgtcgctggt ttgcgcgttg agcgagccga
      121 ccgcgacgtt ttgcagcgac agatgtccgt cgccggactg cagcgcgagc gaatcgccgt
      181 gcacgcgggt cgccgaaacg gaaccgtcgt cgctgcgtcc gtcgaccgtg ccgcgcaaat
      241 cggtgagcgt cacgtgcccg tcctgcgaac ggacggccac gccgccctcg atgccggcga
      301 cgtcggcacc cgcacagcgc gcgatctcga cgtgggacgc ggccggaacg tcgacctcga
      361 cgcgccgctc gaacgaaccg aagatgaagt gcaagtgaag gtcgtccgaa ccgggacgtg
      421 agaccgatac gccgtcggcc gtgcgctgca cttgcagctg cgcgacgtgc catcgatcgc
      481 cgaagatgct gccgctgacg ctggtcagat cgctcacgtg cacttggccg tcgctcgagg
      541 ttgcgacgac gatccgcgag ttcgggtcgt cgaccgtaat ccggggcgcg ctgcccgcgt
      601 cgattggtgc gatcggctta gcggcgaaag cgacttgatg aaagttggcg aagttggcgc
      661 tgccgcggat cgcgtagagc gcgatcccga cgatgacgat ttcggcggcg atcagcatgc
      721 cgacgatcga ggcacgggac atgcttagat cctcggaccc cagccggtga aggtggggcg
      781 caacgactgg gcggagttcc gctccggtcc gtgtaccgtc gggcgggcct caggaagagt
      841 gtagttcggg atgatcacca tgatcgggtc gagcctcctt gggaatgtct gccctagtta
      901 cggccggcgg ggccacttgt ttcgattggg ccgagccagg cgagccggcc tcgcgggcgc
      961 gcgaaacaac ggcccggcga gcgccgtacc tcttacgtgc cggtacttgc ggaggccccg
     1021 cgcctcgatc gtatgagggt cggcccggaa gatggcgact tggtggcgat gacgctccgg
     1081 ggcaacccgg aagcgttcgc tacgctggtc gagcggtacg accgggcggt gtatcacctg
     1141 gcctaccgca cgctgcacga cgtcgaggat gcccgggacg cgacgcagga agcctttttc
     1201 aaagcgttcc ggagcctgcg gacgttcaag ccgggcgcgc ggttctcgac ttggatcttt
     1261 gcaatcgcct atcacgcttg ctgcgatcgt ttgaaccggc gcaagcgtta cagtaacgag
     1321 gagctgccgg accgcgccga cgcggggccc ggaccggaac agcaggcgat cgcgctcgac
     1381 gaggcggcgc gcctgcgcac cgcgatcgac g
//
LOCUS       DASUCJ010000411         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_691943, whole genome shotgun sequence.
ACCESSION   DASUCJ010000411 DASUCJ010000000
VERSION     DASUCJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691943"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFU90_04910"
     CDS             <1..1232
                     /locus_tag="VFU90_04910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5479149.1"
                     /translation="GETVTEGTVAQWLKNVGDPVEKYEAFVEVSTDKVNAEVPSPVTG
                     TIREVLVKEGETVATGTPIAVIDEVGAAVAPAAPAAEAPPPPAAAAAPAQAPSTNGSA
                     AAGSPNGAAEPALRGASPAVRRLAREHRVDVRAIKGSGANGRVTAEDVLAAARAGSAT
                     APPRAPAAAGAPSYAAPVAGATIPLTQARRIIAERMVESKHTAPHAWSMVQIDVTNVW
                     KWRAREKDRFERETGYKLTLLPFFIRAAVESLAAFPLMNAKFTPDGIYVNEDVNVGIA
                     IGLATNLVVPVIRRADQLSIRGLAIAAGELIERARKGKLNVDDLAGGTFTVNNNGANG
                     SWASAPIINAGQAGILTMETVVKQPVVIEGDAIAIRHIMNSCLSLDHRVVDGYVASGY
                     LADLKRRLERMGPEGSL"
     gene            1259..1645
                     /gene="queD"
                     /locus_tag="VFU90_04915"
     CDS             1259..1645
                     /gene="queD"
                     /locus_tag="VFU90_04915"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEU5479150.1"
                     /translation="MQIRRHFRFEAAHVLPFHAGKCARMHGHSYRLEVAVRGPVQTDG
                     PARGMIVDFDEIRRIVHHTAIDPLDHQTLNDFIENPTAERIVLWIWERVDPVLAGLDE
                     LVLWETSNSCAVLRRSDFHAAGPAER"
     gene            1591..2220
                     /locus_tag="VFU90_04920"
     CDS             1591..2220
                     /locus_tag="VFU90_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEU5479151.1"
                     /translation="MRGLTAIGFPRGRTGRTLMLALAEVFYSVQGEGGYAGTPAVFVR
                     LAGCNLACDFCDTDYSTKAIVSVDDVVRMVREAGGSCPSVILTGGEPLAQTETAELID
                     ALRADGRRVHVESNGTIFVGLPTDVWLCVSPKERVDPRMAQRANEVKLIVDGRVPEEH
                     LPLFAGKPAILLQPEGNKPANVAIALDYVKAHPQRFRLSLQTHKFIGIP"
BASE COUNT          417 a          813 c          774 g          380 t
ORIGIN      
        1 tcggcgaaac cgtgaccgaa gggaccgtcg cgcaatggct caagaacgtc ggcgaccccg
       61 tcgaaaaata cgaggccttc gtcgaggttt ccaccgacaa ggtcaacgcc gaagttccct
      121 cacccgtcac cggaacgatt cgcgaagttt tggtcaagga aggcgaaacg gtcgccaccg
      181 gcacgccgat cgccgtgatc gacgaagtcg gtgccgcggt tgcgccggcc gcacccgcgg
      241 cggaagctcc gccgccgccc gccgccgccg ccgcgcccgc gcaggcaccc tcgaccaacg
      301 gcagcgcagc ggccggatct cccaacggcg cagccgagcc cgcgttgcgc ggcgcctctc
      361 ccgcggtacg ccggctcgcg cgcgagcacc gcgtcgacgt ccgtgcgatc aaaggttccg
      421 gcgccaacgg ccgcgttacc gccgaagacg tgctcgccgc cgcgcgcgcg ggcagcgcga
      481 cggcaccgcc ccgcgcgccc gccgccgccg gggcgccgag ctacgcagcg ccggtcgccg
      541 gcgcgaccat tccgctcacg caggcgcgcc gcatcatcgc cgagcgcatg gtcgagagca
      601 aacacaccgc tccgcacgcg tggtcgatgg tccaaatcga cgtgacgaac gtttggaaat
      661 ggcgcgctcg cgaaaaggac cggttcgagc gcgagaccgg ctacaagctc acgcttctgc
      721 cgttcttcat tcgcgctgcg gtcgaatcgc tcgccgcctt cccgctgatg aacgcaaaat
      781 tcacgcccga cggtatctac gtcaacgagg acgtcaacgt cggaattgcg atcgggcttg
      841 cgacgaactt ggtggtgccg gtcatccggc gcgccgacca gctgtcgatt cgcggcctcg
      901 cgatcgcggc gggcgagttg atcgaacgcg cgcgtaaggg gaaactcaac gtcgacgatc
      961 tcgccggcgg gacgtttacc gtcaacaaca acggcgccaa cgggtcgtgg gcgtcggcgc
     1021 cgatcatcaa cgccgggcaa gcgggaattc tcacgatgga gacggtcgtc aagcagccgg
     1081 tcgtgatcga aggcgacgcc atcgccatcc ggcacatcat gaactcctgc ctgtcgctcg
     1141 atcaccgcgt ggtcgacggc tacgtcgcga gcggctacct ggcggacctc aagcggcggc
     1201 tggagcgtat gggccccgaa ggctcgctgt aaaacccgac gccgcccgag ccgtttcgtt
     1261 gcagattcgc cgacactttc gtttcgaggc ggcgcacgtg ctgccgtttc acgccggcaa
     1321 gtgcgcccgg atgcacggac attcgtaccg cctggaagtt gccgtacgcg gtcccgtgca
     1381 aaccgacgga cccgcgcgcg gaatgatcgt cgatttcgac gagatccggc ggatcgtgca
     1441 ccataccgcg atcgacccgc tggatcatca aacgctcaac gactttatcg agaatccgac
     1501 cgccgaacga atcgtgttgt ggatttggga acgcgtcgat ccggttctcg ccggcttgga
     1561 cgagctggtg ctctgggaaa cctcgaactc gtgcgcggtc ttacggcgat cggatttcca
     1621 cgcggccgga ccggccgaac gctgatgctc gcgctggccg aagtcttcta cagcgtgcag
     1681 ggtgaaggcg gctacgccgg aacgccggcc gttttcgtca ggctggcggg ctgcaatctg
     1741 gcctgcgatt tttgcgatac cgactactca accaaggcga tcgtctcggt cgacgacgtc
     1801 gtccggatgg tgcgcgaagc cggcggctcg tgtccgtcgg taattctcac cggcggcgaa
     1861 ccgctcgcgc aaaccgaaac cgccgagctg atcgacgcgc tgcgcgccga cggccggcgc
     1921 gtgcacgtcg aatccaacgg gacgattttc gtcggcctgc cgaccgacgt ttggctgtgc
     1981 gtcagtccca aggagcgcgt cgacccgcgc atggcgcagc gcgcgaacga ggtaaagctg
     2041 atcgtcgacg gacgcgttcc cgaggaacat ctgccgctct ttgccggtaa gccggcgatc
     2101 ttgctgcagc ccgaggggaa caaaccggcc aacgtcgcga tcgcgctcga ttacgtcaag
     2161 gctcatccgc agcgcttccg gctgtcgctg cagacgcata aattcatcgg aataccttag
     2221 gacgctcggc taacgtgatt ttgctgacgg ccgccgtcga acgcgaattg agccgctggg
     2281 ccggccggcc cggtgtcgag accgtcgtca cgggcgtggg cccggtcgaa gcggcctgcg
     2341 cgatcgccga tcgtttggcc cggcagcgct acgagctcgt cgtg
//
LOCUS       DASUCJ010000412         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_463093, whole genome shotgun sequence.
ACCESSION   DASUCJ010000412 DASUCJ010000000
VERSION     DASUCJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463093"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFU90_04925"
     CDS             <1..1014
                     /locus_tag="VFU90_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5479152.1"
                     /translation="IRHEPRDRELPAVDVERLFPKVLAHRDVCSREPLYRRYDWVVRG
                     ATVLPRGAADAGVLAPVPKSTLGVALSVAGNPRYGRIDARYAAELAVLESVRRVVAVG
                     ARPIGLTDCLNFGNPRKPEQYGEMIAAIEGLDRAAREMGLAFVSGNVSLYNESADGSA
                     IPASAIVACIGALTDVSRTITPGLKRSGSSLLWIGSRELAVGGSVLADLLGIEGPLPE
                     ISYDAERAAIGLVESAMSSCVLLACRAVSDGGMLTALARLAFAAHQAGRPLGAELDFG
                     NPFCEAGGFLCEVSDDSALDLSGILKVGTTIDEPRLIVNGTAFDVGALHETWSAPLAE
                     IYP"
     gene            1011..1781
                     /locus_tag="VFU90_04930"
     CDS             1011..1781
                     /locus_tag="VFU90_04930"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5479153.1"
                     /translation="MTHRIAVLVFPGTNSEDETLRLLRDCGGEAELVHWSRGETLGSF
                     DAYVLPGGFAYEDRIRAGAIPAHDRMMDFVIAGALEGKLVLGVCTGAQILLEAGLVPG
                     TGAVRRPTAAFTDNGPVPHFVCRHVYVKLALPPARSPITAALPDDAVIPAYAANGEGR
                     LAASREHLAEIVAGGHLAFVYAQADGSAGTAAVPNESALGCAGLINAAGNVLAIMPHP
                     ERDAWNFNHLDRSEGSDALAPSGGAALFRSFVAAVNAR"
     gene            1778..2221
                     /locus_tag="VFU90_04935"
     CDS             1778..2221
                     /locus_tag="VFU90_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479154.1"
                     /translation="MSLRVVAIELKIPDNAAFTALVALRRLGLPVERVERSEIWRFED
                     AGDPKTFAARVRANQTIFNPNKHRLVELERAVPRAGEAWIEKASEGNEVREHLGGKGI
                     DGITASHRSVGWRLLGSNGAPAARETLAAAVTGLLCNPAIEKAVY"
     gene            2229..>2889
                     /locus_tag="VFU90_04940"
     CDS             2229..>2889
                     /locus_tag="VFU90_04940"
                     /inference="COORDINATES: protein motif:HMM:NF018548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1246 domain-containing protein"
                     /protein_id="HEU5479155.1"
                     /translation="MNRPYTIATLGSHSALQILKGAHDEGFKTLAISNRDTERLYRSF
                     AFVDDVIALENYSEFMTCVDELARRKTIVVPHGSFVAYLSLEEHKKMTVPYFGNKAVL
                     DWEASRELQRQWLSRAGLTLPRQFKSGAEIDRPVIVKLYGAAGGKGYMFIRDTNDFEA
                     RAAHLKEDYIIQEYVIGVPLYIHYFYSPLKNTLEIMSMDRRYETNVDSLGRIPADAQG
                     GM"
BASE COUNT          481 a          929 c          993 g          486 t
ORIGIN      
        1 attcgccacg aaccgcgcga ccgcgagttg ccggcggtcg acgtcgagcg gcttttcccg
       61 aaagttctcg cccatcgcga cgtgtgctcg cgcgaaccgc tgtaccgccg gtacgactgg
      121 gtcgttcggg gcgcgacggt actgccgcgc ggcgccgccg acgccggcgt gctcgcgccg
      181 gttccgaagt cgaccctggg cgtcgctttg agcgtcgcgg gcaacccgcg atacggccgg
      241 atcgacgcgc ggtacgccgc cgagctcgcc gtgctcgaat cggtgcggcg cgtcgtcgcc
      301 gtcggtgcgc gtccgatcgg tttgaccgat tgcctgaact tcggtaaccc gcgcaaaccg
      361 gagcagtacg gcgagatgat cgccgcaatc gaaggattgg atcgtgcggc ccgcgagatg
      421 gggctggcct tcgtctcggg caacgtgagc ttgtacaacg agtcggcgga cggctcggcg
      481 attccggcgt cggcgatcgt ggcctgcatc ggcgcactta cggacgtttc gcggacgatc
      541 acgccgggtc tcaaacgctc cggctcgtcg ctgttgtgga tcggcagccg cgaactggcg
      601 gtcggcgggt cggtactcgc cgatctgctg ggaatcgaag gaccgctgcc cgagatttcg
      661 tacgatgcag agcgcgccgc catcggcttg gtcgaaagcg cgatgtcgtc ctgcgtgctg
      721 ctcgcgtgcc gcgcggtctc ggacggcggc atgttgacgg ccctcgcgcg gctcgcgttc
      781 gcggctcatc aagccgggcg tcctttgggc gccgagctgg actttggaaa tccgttctgc
      841 gaagcgggcg ggttcttgtg cgaggtcagc gacgacagcg cgctcgacct cagcgggatc
      901 ctcaaggtcg gcacgaccat cgacgagccc cgcctgatcg ttaacggcac ggcgttcgac
      961 gtcggggcgc tgcacgaaac gtggtcggcg ccgctcgcgg agatctatcc gtgacgcacc
     1021 gcatcgccgt cttggtgttt ccgggcacca attcggaaga cgaaacgctg cggttgctgc
     1081 gcgattgcgg cggcgaggcc gaactggtgc attggtcgcg cggcgaaacg ctcggaagtt
     1141 tcgacgcgta cgttttgccg ggagggtttg cctacgaaga ccgcattcga gcgggcgcga
     1201 tcccggcgca cgaccggatg atggatttcg tgatcgccgg agcgctcgaa ggaaagctcg
     1261 tgctcggggt atgcaccggc gcccagattc tgctggaggc cgggctcgtg cccggcacgg
     1321 gcgcggtgcg ccgcccgacc gcggcgttta ccgacaacgg tccggttccg catttcgtct
     1381 gccgccacgt ctacgtgaag ctcgcgcttc cgccggcgcg cagcccgatc accgcggcgc
     1441 tgccggacga tgcggtgatt ccggcctacg ccgccaacgg cgaggggcgg ctcgcggcct
     1501 cgagggagca cctcgcggag atcgtcgccg gcgggcattt ggcgttcgta tacgcgcaag
     1561 ccgacggcag cgccggcacc gccgcggtcc cgaacgaatc ggcgctcggc tgcgcgggat
     1621 tgattaatgc ggccggcaac gtgctcgcga tcatgccgca tcccgagcga gacgcgtgga
     1681 acttcaacca cctcgatcgc agcgaaggga gcgacgcgct cgcgccgtcg ggcggggcgg
     1741 cgctgtttcg cagcttcgtc gcggcggtga acgcgcgatg agccttcgcg tggtcgcgat
     1801 cgaactgaag attcccgaca acgccgcgtt caccgctctc gtggcgctgc gccggctggg
     1861 gctgccggtc gaacgcgtcg agcgcagcga gatctggcgc ttcgaagacg cgggagatcc
     1921 gaaaacgttc gcggcgcgcg tgcgcgcgaa ccaaacgatc ttcaacccca acaaacaccg
     1981 gctggtcgag ctcgaacgcg ccgtgccccg tgccggggag gcgtggatcg agaaagcaag
     2041 cgaaggcaac gaggtacgcg agcacttggg cggcaaaggc atcgacggca tcacggcgtc
     2101 acaccggtcc gtcggctggc gcctgctcgg ttcgaacggt gcgccggctg cgcgagaaac
     2161 gctcgccgcc gcggtaacgg ggctgctctg caatccggcc atcgagaaag cggtttatta
     2221 gaggaagcat gaacaggccg tacacgatcg cgacgctcgg ctcgcactcg gcgctgcaga
     2281 tactcaaggg cgcgcacgac gagggattca agaccctcgc gatctcgaac cgcgataccg
     2341 agcggctcta ccggtcgttc gcgttcgtcg acgacgtcat cgccctggag aattactcgg
     2401 agttcatgac ctgcgtcgac gagctggcac gccgcaagac gatcgttgtc ccgcacggat
     2461 ccttcgtggc ctatctctcg ctcgaagaac acaagaagat gacggtcccc tacttcggga
     2521 acaaggccgt gctcgattgg gaggccagcc gggagctgca gcgccagtgg ctgtcgcgcg
     2581 cgggccttac gctgccgcgg cagttcaaga gcggcgccga gatcgaccgg ccggtgatcg
     2641 tgaaactcta cggcgccgcg ggcggcaagg gctatatgtt cattcgcgac accaacgatt
     2701 tcgaagcgcg cgccgcccat ctcaaagaag attacatcat tcaggagtac gtcatcggcg
     2761 ttccgctcta catccactac ttctattcgc cgttaaagaa tacgctggag atcatgtcca
     2821 tggaccggcg ttacgagacc aacgtcgatt cgctcggacg aattccggcg gacgcgcaag
     2881 gcgggatgg
//
LOCUS       DASUCJ010000413         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_371421, whole genome shotgun sequence.
ACCESSION   DASUCJ010000413 DASUCJ010000000
VERSION     DASUCJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371421"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFU90_04945"
     CDS             complement(<1..936)
                     /locus_tag="VFU90_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479156.1"
                     /translation="MRRTTLAAGAVIALAACSTSGRLTGNNINLAPPNNLTYQLIPSG
                     NADFPEGIDLRWDPVNDPNIADYAVFSRSSANGAWNLRAKTTSPSFEDLGAPDLQYYV
                     ASEAADGSMSAPSNVITVDNTNKLPPPDSLEAFALDQAVELSWSPNARLSNPSAFSYY
                     RVYSTVYHAGQNLCDTAWVLEGTTVSEDFIASGLPNGQPRCFDVSAISIDGHESDWPT
                     PVAATPSATAGDIVVFSSADSLNKSGFVFPSSASTRSVVASATRPDIDFRVDRHADGS
                     MWLVPAKSGTTVALYSSQPLASISAFGVAPSTGYSS"
BASE COUNT          184 a          333 c          358 g          176 t
ORIGIN      
        1 ggaggagtat ccagtgctcg gcgcaacacc gaaggcggag atgctggcga gcggctgcga
       61 gctgtacagc gcgacagtcg tccccgattt cgccggcacc aaccacatgc ttccatcggc
      121 gtgccggtcg acacggaagt cgatgtccgg gcgcgtcgcg ctcgccacga cgctgcgcgt
      181 gctcgcggac gacgggaaga cgaacccgct cttgttcaac gaatcggccg acgagaacac
      241 cacgatgtcg cccgccgtgg cgctgggcgt ggccgcgacc ggcgtcggcc aatcgctttc
      301 gtggccgtcg atgctgatgg cgctcacatc gaagcagcgc ggctgcccgt taggcaagcc
      361 gctggcgatg aagtcctccg acacggtggt gccctcgagc acccaggcag tgtcgcagag
      421 attctgtccg gcgtgataga cggtcgaata cacccgatag tagctgaacg ccgacgggtt
      481 cgacagccgt gcgttaggcg accacgacag ctccacggcc tggtcgagcg cgaacgcttc
      541 gaggctgtcc ggcggcggca gcttgttggt gttgtcgacg gtgatgacgt tggatggcgc
      601 gctcatcgac ccgtcggccg cctcggaggc gacgtagtac tggagatccg gtgcgcccag
      661 atcctcgaac gacggagacg tggttttcgc acggagattc cacgcgccgt tcgccgagga
      721 ccgcgagaac accgcgtagt cggcgatatt gggatcgttc accgggtccc accgcaaatc
      781 gatcccctcc ggaaaatcgg cgttcccgct cgggatcagc tggtacgtga ggttgttggg
      841 cggggcgagg ttgatgttgt tcccggtcag gcggcccgag gtcgagcaag cggcgagtgc
      901 aatcacggca ccagccgcca gagtcgtgcg gcgcattgca ggctccattg gcgtatgcgc
      961 ccctgcagtg cacgccccgc gccgagcgtt gcggcgcggc aaaatcaatg acatcaaggc
     1021 gttactttcg aacgcaccgc ggacgccgtc c
//
LOCUS       DASUCJ010000414         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_355951, whole genome shotgun sequence.
ACCESSION   DASUCJ010000414 DASUCJ010000000
VERSION     DASUCJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355951"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFU90_04950"
     CDS             <1..1081
                     /locus_tag="VFU90_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479157.1"
                     /translation="ASVPNAFVVSPPQSYAGGTATLHVTANFSGQSANPCVQPGASCS
                     GNVTLDVRQLLAVANSSINTVTLYAGAQSTPLVTIQGGVTNPQALVFDAAGDLFVASQ
                     PGTVTEYTVPYTGTPSTIALGVNHPQAMALDARGNLFVANGSASNTVTEYAPPYSGAP
                     IATISTGIDDPVSLGLDSAGNLFVSNQAANTVTVYAPPYTGANIALSNGLNGPNSLAI
                     DGRGNLFVSNLNSTPNSVVEFVPPFSSGSIPTVTVANGVNEQGTIGLSTAANLFVPNQ
                     GANTVTEYAPPYAGTPTTISGGQSQPVALAIDVSGNLFVANYGNNTVTEYAPPYAGAS
                     WITFSNGVNSPQALALSPPTTSLIP"
     gene            complement(1089..3023)
                     /locus_tag="VFU90_04955"
     CDS             complement(1089..3023)
                     /locus_tag="VFU90_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5479158.1"
                     /translation="MDHACQNCGARPAPVFDGTRRLCTLCAQKRTLTGALPFLGAALA
                     AAGLIAGSMLLAEKMQGDANERGSSPLDELTKRFRGTPTLGNFSRDLTDLARAGKLDP
                     VIGRDDEIERLVSILARRSKNNPVLVGEPGVGKTAIVEGLAQRIVAGNVPASLRGKRV
                     LALSLGPLVAGTKYRGEFEGRVKRILDEVKRSARDVILFIDELHTLVGAGAAEGALDL
                     SSMIKPELARGELQCIGATTFDEYRKYIESDAALERRFQPVMVEEPSIEQTIAILNGL
                     RDGYAQHHRVAIGDGAVDAAATLSARYIADRFLPDKAIDLMDEAAASVALQNKGAVDR
                     PEVTRDHVAAVVTRWTGIPQAALSEAQANRLLDLERVLGKRVIGQDLAIAAASDAIRR
                     ARAGLHDPRKPLGSFLFKGPSGVGKTELAKALAEALFGSEAALVRIDLSEFTEQHSVS
                     RLLGAPPGYAGHDEPGQLTEPVRRRPYCVVLFDELEKAHPDVAAVLLQILDDGRVTDA
                     KGRTIDFRHALIVLTTNLEDDELPFALRPELVNRIDEIVAFRPLGLPEIEAIVAIHVD
                     ALAARLGARDVRLRLSEEARRFLADESMAAGTGARFVQRTVSRHVSTPLSSAILRGVL
                     QAGSTAGVDLQDGTLVVRAA"
     gene            complement(3076..>3473)
                     /locus_tag="VFU90_04960"
     CDS             complement(3076..>3473)
                     /locus_tag="VFU90_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5479159.1"
                     /translation="HPDGERYGYTVSRGFNVKVRDIAKAGRVADACTSSGATSLNGVT
                     FGLADSTAARNSATKKAVDDARTYAEALAAAAHLRIVGIEKIDLGGGGMVAPAPLMRM
                     AANAATPTQFDQSNVSVSVSVTVTFLAEP"
BASE COUNT          568 a         1175 c         1167 g          563 t
ORIGIN      
        1 ggcctcggtg ccgaacgcat ttgttgtatc gccgccgcag tcgtatgccg ggggcaccgc
       61 cacgctgcac gtcaccgcca atttttccgg acagtccgcc aatccgtgcg tgcagccggg
      121 agcgtcgtgc agcggaaacg taacgctgga cgtacgccag ctgctcgcgg tcgcgaactc
      181 gagcatcaat acggtcacgc tgtacgccgg cgcgcagagc acgccgctcg tcaccattca
      241 aggcggcgtc accaacccgc aggcgctggt gttcgacgcg gccggcgacc tgttcgtcgc
      301 gagccagccg gggaccgtga ccgaatatac ggtgccgtac accgggacgc cgtcgacgat
      361 cgcgctcggc gtcaaccatc cgcaggcgat ggccctcgac gcccgcggga acttgttcgt
      421 cgccaacggc agtgcgagca acacggtgac cgaatacgcg ccgccgtact cgggcgcacc
      481 gatcgccacg atctcgacgg ggatcgacga tccggtgagt ctggggctcg actcggccgg
      541 caatctgttc gtctcgaatc aggccgcgaa caccgtcacc gtgtacgctc cgccctatac
      601 cggcgcgaac atcgcgctgt cgaacggatt gaacggaccg aactcgctgg cgatcgacgg
      661 gcgcggcaac ttgttcgtct caaacctcaa cagcacgccc aactccgtgg tcgagttcgt
      721 gccgccgttt tcgagcggca gcatccccac cgtcacggtc gccaacggcg tcaacgaaca
      781 aggcacgatc ggactgagta ccgcggccaa cctattcgtt cccaatcagg gcgcgaatac
      841 ggtgaccgaa tacgcgccgc cgtacgcggg aactccgacg acgatcagcg gcggtcaaag
      901 ccagccggtc gcgctcgcga tcgacgtctc cggcaactta ttcgtcgcca actacggcaa
      961 caacacggta accgaatacg cgccgccgta cgccggcgca tcgtggatta cgttttcgaa
     1021 cggcgtcaac agcccgcagg cgctggcgct ctcgccgccg acgacgagtt taataccgta
     1081 gcgggcggct aagcggcccg gacgacgagg gtgccgtctt gcagatccac gccggcggtg
     1141 ctgccggctt gcagcacgcc gcgcagaatc gccgacgaca gcggcgtcga gacgtgacgc
     1201 gatacggtcc gttggacgaa acgcgcgccg gtgccggccg ccatcgactc gtcggctaag
     1261 aatcttcgag cctcttccga cagtcgcagg cgcacgtcgc gcgcgccgag ccgggcggcc
     1321 agcgcgtcga cgtggatcgc gacgatcgct tcgatctcgg gcaagccgag cggacggaag
     1381 gcgacgattt cgtcgatgcg attgaccagc tcgggacgca gcgcgaacgg cagctcgtcg
     1441 tcttcgagat tggtcgtaag cacgatcagc gcgtggcgga agtcgatggt gcggcctttc
     1501 gcatcggtga cgcggccgtc gtcgagaatc tgcaggagca ccgcagcgac gtcggggtgc
     1561 gctttttcga gctcgtcgaa gagcacgacg cagtacgggc ggcggcgcac cggctcggtg
     1621 agctgaccgg gctcgtcgtg gccggcgtat cccggcggcg cgcccagcag ccgcgaaacc
     1681 gagtgctgct cggtgaattc cgagaggtcg atgcgcacca gcgcggcttc gctgccgaac
     1741 agcgcttcgg cgagcgcctt cgcgagctcg gttttgccga cgccgctcgg ccctttgaag
     1801 agaaaactgc ccagcggctt gcgcggatcg tgcaacccgg cgcgcgcgcg gcggatcgcg
     1861 tccgacgcgg cggcgatcgc gaggtcttga ccgatgacgc gtttgccgag cacccgctcg
     1921 agatcgagca ggcggttggc ttgcgcttcg ctcagcgccg cctgcgggat gccggtccag
     1981 cgcgtcacga cggcggccac gtgatcgcgg gtgacttcgg gccgatcgac ggcgccttta
     2041 ttctgcagtg cgaccgacgc ggcggcttcg tccatcagat cgatggcttt atcgggaaga
     2101 aaacggtcgg cgatgtagcg cgccgacaac gtagccgcgg cgtcgacggc gccgtcgccg
     2161 atggcgacgc gatggtgctg ggcgtagccg tcgcgcaggc cgttgagaat cgcgatcgtc
     2221 tgctcgatgc tcggctcttc caccatgacc ggctggaagc gccgctccag cgcggcatcc
     2281 gactcgatgt acttgcggta ctcgtcgaag gtggtcgcgc cgatgcactg cagctccccg
     2341 cgcgcgagct cgggcttgat catcgagctc aagtcgagcg cgccttcggc cgcgcccgca
     2401 ccgaccagcg tatgcagctc gtcgataaac aagatcacgt cgcgggcgct gcgcttgact
     2461 tcgtcgagga tgcgtttgac gcgcccttcg aactcgccgc gatacttcgt acccgcgacc
     2521 agcgggccga gcgagagcgc gagcacgcgt ttgccgcgca gcgaggccgg aacgttgccg
     2581 gcgacgatgc gctgggccag gccttcgacg atggcggttt tccccacgcc gggctcgccg
     2641 acgagcaccg ggttattttt gctgcggcgc gcgagaatcg agacgagccg ttcgatctcg
     2701 tcgtcgcgtc cgataacggg atcgagcttt ccggcgcgcg ccaagtcggt gaggtcccgc
     2761 gaaaagtttc ccagcgtcgg ggtgccgcga aagcgcttcg tcaactcgtc gagcggcgag
     2821 ctgccgcgct cgttcgcatc gccctgcatt ttctccgcca gcagcatgct gccggcgatc
     2881 aagccggcgg ccgctaaggc cgcgccgagg aacggaagcg ctccggtcag cgttcgcttt
     2941 tgagcgcaga gggtgcagag ccggcgagtc ccgtcgaaga cgggcgccgg ccgagccccg
     3001 cagttttgac acgcgtggtc catcgatgcg atgtacccta gccgcccgcc cggagtttcg
     3061 ccggccgccg cagggctacg gctcggccag gaacgtgacg gtgaccgaga ccgaaacgct
     3121 cacgttggat tggtcgaact gggtgggggt cgcggcgttg gccgccatcc gcatcagcgg
     3181 cgcgggcgcg accatcccgc cgccgcccag atcgatcttc tcgatgccga cgatccgcaa
     3241 gtgcgccgcc gcggccagcg cttcggcgta ggtgcgcgcg tcgtcgacgg ctttcttagt
     3301 ggcgctgttg cgcgccgccg tcgaatccgc caacccgaag gtcacgccgt tgagcgacgt
     3361 cgcgcccgaa ctcgtgcaag cgtcggccac gcgcccggcc ttcgcgatgt cgcgtacctt
     3421 cacgttgaaa ccacggctga cggtgtagcc gtagcgttcg ccgtcggggt gcg
//
LOCUS       DASUCJ010000415         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_386409, whole genome shotgun sequence.
ACCESSION   DASUCJ010000415 DASUCJ010000000
VERSION     DASUCJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386409"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="VFU90_04965"
     CDS             complement(<1..1600)
                     /locus_tag="VFU90_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479160.1"
                     /translation="MPAFQQDWAWPLSRNLSLQWLHAFAGLWDPRSLGQGNPLPWQTY
                     AVFVQVACILVFGPAAGLALWIAALEFLAGYGCVRLLGSFGVRSPLARFVPALFYALS
                     PVTFTRIAAGHLAYLLAYALLPLALWLARRTIESPRAMFVPALGVAVGLAGSQIQFLA
                     IAWLAILPLAAAISRAPGWSARLAIAAAISLCVQLQALLPLAFSSAPSLYAAQPALLS
                     FEYNNSSPFASAPVMLGYFTHYYESHALVGTFAVLYALLAGAVLLALWAQRRAGLYAV
                     ALVAIGTVLTAGLYGPLSVPLAWAFERVAYFAVFRDLHYFAALTAAGIALALGLGLQR
                     APRALAWGALAAAVWIAVPVLGGAELRELLVPRAYVDDVLADMAAVAQHGPGRVLWLP
                     AEEPLGLRGAGIQGRDFTAYGPAGNPSVSDDYQNPQLAYALATLRSGRPDWKSFAAMN
                     VRYLVFRNYVKSGRTENFGTGLPMAFSGLSDAGIGILLQGTPRLIRLRSSASSTVYEL
                     PGYAGPTYVARTDAGARLFSQLNAG"
     gene            complement(1696..>4131)
                     /locus_tag="VFU90_04970"
     CDS             complement(1696..>4131)
                     /locus_tag="VFU90_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479161.1"
                     /translation="DNTFIGVPRLVYHLGIDALIATFHNWQMAQWIWYSSMSALGLFG
                     AFALARRLGAGIYSIPLAIFYAFNLWSYDRIAQGPIYLSYQAMPLVIYLLLRYLARPS
                     VAAALYFACSLLFVIPALQISYLAALICLGIAVREVVLRGWRVIGKLALLAVAVVAAN
                     AFYVFSMLADLWLNAGGNIALVNQRFDVGIFLHYAENVSVFNTLRLTSFYYTSLERQL
                     PVVQFAVVLIPLLLLSLLLLARKPTVRSKFYGGVALALLGIWLVDGIVIAPAFYQWFR
                     NAIPGLRSFVEPDYYSPLYVAGAFVMLASALRLGARAYGLWWKIGVWIAALTGVLAFL
                     PINGPESGMPQTGQPRQYAEFSRSKVRGSTLWIPPERGVEYRWSPYVINGFTSLNSPS
                     DAIGPTMAEWVAPGTARVQARLANGFMAGQLNTVETLAPLLGVGTVAIAADSLGQLGE
                     WPNPEITGALQTLSALQDRGFLSLRGDYRDERVRLVTATIASTLPELGVYDAPVDIGG
                     FDNYMWLLALSKTGTAPYTPVAADAPPGASYGGRPMRFVAVPAPHLRYVPIEAFAGAS
                     CSLPAKRHRTPYGPAPRSLRTEMLPRCFVLPLSRIREIAALTVNVDGRLPELAPPGDE
                     LQPQLIFDGNGSDADWIDPRLAAQEVPARARSAAIRIDVPPYTLAVLRGVTIRWAARG
                     QTAAIPPVPTCATSQVRWSEQNPLVYTLRGNVNGRCTLVFRQSFAPIWTLFASGGSAK
                     VLGHLQIDGFANGWIVDASGPVTFRIVNRAIFAYAAGMILTLASVLLALGFALHARLT
                     RAEPRESRVPSPA"
BASE COUNT          806 a         1391 c         1396 g          538 t
ORIGIN      
        1 cgcccgcgtt cagctgcgaa aaaaggcgcg cgccggcgtc ggttcgcgcg acgtacgtcg
       61 gccccgcgta gccgggcagt tcgtaaacgg tcgacgacgc cgacgaacgc agccggatca
      121 gccgcggcgt accctgcagc agaatcccga tcccggcgtc gctcaagcct gaaaacgcca
      181 tcggaagacc ggtgccgaag ttctcggtac gccccgactt cacgtagttg cgaaacacga
      241 gataccgcac gttcatcgcg gcgaacgact tccagtcggg ccgcccgctt cgcagcgtcg
      301 cgagtgcgta ggcgagctgc ggattctggt agtcgtcgct caccgacggg tttcccgcgg
      361 gtccatacgc cgtgaagtcg cggccttgga tgcccgcccc gcgcagaccg agcggttctt
      421 cggcgggaag ccaaaggacg cgtcccggcc cgtgctgcgc gaccgcggcc atgtcggcca
      481 gcacgtcgtc gacgtacgcg cgcggcacca gcagctcgcg cagttccgca ccgccgagca
      541 ctggaacggc gatccagacg gccgccgcca gcgcacccca cgcgagcgct cgcggcgcgc
      601 gctgcaggcc gagcccgagt gcgagcgcga tgccggcggc ggtcagtgcg gcgaagtagt
      661 gcaggtcgcg gaagaccgca aaatacgcga cgcgctcgaa cgcccacgcg agcggaacgc
      721 tcaacggccc gtacagtccg gccgtcagca ccgtcccgat cgcgaccagc gcgacggcat
      781 agagaccggc cctccgctgc gcccaaaggg ccagcagcac ggcgcccgcg agcagcgcgt
      841 agagtacggc gaacgtcccc acgagcgcgt gcgactcgta gtagtgggtg aaatatccga
      901 gcatgaccgg cgccgatgca aacggcgagc tattgttgta ctcgaagctc agcagtgcgg
      961 gctgggcggc gtaaagcgac ggcgccgagc tgaatgcgag cgggagcagc gcctgcagct
     1021 gcacgcagag cgaaatcgcg gccgcgatcg ccaatcgcgc ggaccagccg ggggcgcgcg
     1081 aaatcgcggc cgccagcggg aggattgcaa gccaggcgat tgccagaaac tggatttgcg
     1141 aaccggccag cccgaccgcg accccgagcg ccggcacgaa catcgcgcgc gggctttcga
     1201 tcgtgcggcg tgccagccaa agcgccaacg gcagcagggc atacgccagc aggtaggcga
     1261 gatgtccggc cgcgatccgc gtgaacgtaa ccgggctcaa agcgtaaaac agggccggca
     1321 cgaaccgtgc gagcggcgaa cgcacgccga aggatccgag cagccgcacg caaccgtagc
     1381 cggcgagaaa ctcgagcgcc gcaatccaca acgcgagccc ggcggcgggc ccaaaaacga
     1441 gaatgcacgc gacttgcacg aagaccgcat acgtctgcca cggaagcgga ttgccttggc
     1501 cgagcgaacg cggatcccac aatccggcga acgcgtgcag ccactgcagc gagagatttc
     1561 gcgacagcgg ccaggcccag tcttgttgaa aggccggcac ggtttgcgcg accggcagcg
     1621 gccaaaccga ggccgcgcac gcgatcgcga gcagcccgaa cgcggccgcc tctactcggc
     1681 ggtcgagcgg accgcttacg ccggagacgg tactcggctc tcgcgtggtt cggccctggt
     1741 gagtcgcgcg tgcagcgcga acccgagggc cagcagcacg gacgcgagcg tcaggatcat
     1801 gcccgccgcg taggcgaaga tcgcgcggtt cacgatccgg aacgtcaccg ggccggaagc
     1861 gtcgacgatc cagccgttgg cgaatccgtc gatctgcagg tgccccagca ctttcgcgga
     1921 tccgccgctg gcaaagagcg tccagatcgg cgcgaaagac tgccggaaca ccagcgtgca
     1981 gcggccgttg acgttgccgc gcagcgtgta cacgagcgga ttttgctcgg accaccggac
     2041 ctgcgaggtc gcgcacgtcg gcaccggcgg aatcgccgcg gtttgcccgc gcgcggccca
     2101 gcgaatcgtg acgccgcgta acacggcgag cgtatacggc ggaacgtcga tccggatcgc
     2161 cgccgagcgg gcgcgtgccg gcacttcttg cgccgcgagc cgcggatcga tccagtccgc
     2221 atcgctgccg tttccgtcga agatcagctg cggctgcagt tcgtcaccgg gcggcgcgag
     2281 ttccggcagc cggccgtcga cgttgaccgt gagcgcggcg atctcgcgga tgcgtgaaag
     2341 cggcaagacg aagcagcgcg gaagcatctc ggtgcgcagc gatcgcggcg ccggcccgta
     2401 cggcgtccta tgccgcttcg ccggcagcga gcacgaggcg ccggcaaatg cttcgatcgg
     2461 gacgtaccgg aggtgcggcg ccggcaccgc tacaaagcgc atcggccgtc cgccgtagct
     2521 cgcaccgggg ggtgcgtcgg cggcgaccgg cgtgtatgga gcggttcccg ttttcgagag
     2581 cgcgagcagc cacatgtagt tatcgaagcc gccgatgtcg accggcgcat cgtagacccc
     2641 cagctcgggc agcgtggagg cgatcgtcgc cgtcacgagc cgaacccgtt cgtcgcgata
     2701 gtcgccgcgc agcgagagaa agccgcgatc ttgcaacgcg ctcagcgtct gcagcgcgcc
     2761 ggtgatttcg gggttcggcc actcgccgag ctggccgagc gaatcggccg caatcgcgac
     2821 ggtccccacc ccgagcagcg gggcgagcgt ttcgaccgtg ttcagctgcc ccgccatgaa
     2881 tccgtttgcc agccgcgcct gtacgcgcgc cgttccggga gcgacccact ccgccatcgt
     2941 cggcccgatc gcgtcggacg gcgaattcaa cgaagtaaag ccgttgatga cgtacggaga
     3001 ccagcggtac tcgacgccgc gttcgggcgg tatccacagc gtagatccgc gcactttcga
     3061 gcgcgagaac tcggcgtatt gccgcggctg ccccgtctgc ggcattccgg actcgggtcc
     3121 gttgatcggc aaaaacgcca ggacgcccgt aagcgcggcg atccacaccc cgatcttcca
     3181 ccacagcccg tacgcgcgcg cgcccagccg tagggccgag gcgagcatca cgaatgcgcc
     3241 ggcgacgtac agcggcgagt agtagtcggg ttcgacgaac gaccgcagcc cgggaattgc
     3301 gttacgaaac cattggtaga acgccggcgc gatcacgatg ccgtctacca gccagattcc
     3361 cagcagcgcc agcgccacgc cgccgtagaa tttcgagcgc accgtgggct tgcgcgccaa
     3421 cagcagcagc gagagcagca gcagcggaat cagcacgacg gcgaactgca ccaccggcag
     3481 ctgacgctcg agcgaggtgt aatagaagct ggtcaagcgc agcgtgttga acacgctgac
     3541 gttctcggcg tagtgcagga agattccgac gtcgaaacgc tggttgacca gcgcgatgtt
     3601 gccgccggcg ttgagccaca gatcggcgag catcgagaac acgtagaacg cgttggccgc
     3661 gacgacggct accgccagca gcgcgagctt cccgataact cgccagcccc gcaagacgac
     3721 ctcgcggacg gcaattccca agcagatgag cgccgctaaa taactgattt gcagggccgg
     3781 gatcacgaac agcagcgagc acgcaaagta aagcgccgcc gccacgctcg gccgcgccag
     3841 gtagcgcagc agcaggtaga ttaccagcgg catggcctga taggagagat agatcggccc
     3901 ctgcgcgatc cgatcgtacg accacaaatt gaacgcgtag aagatcgcca gcggaatcga
     3961 gtagatgccg gcgccgagcc ggcgcgcgag cgcgaacgcc ccgaatagtc ctagggcgga
     4021 catgctcgaa taccaaatcc actgcgccat ctgccagttg tggaaggtag cgatcagcgc
     4081 gtcgatgccg aggtggtaga ccagccgcgg aacgccgatg aaggtgttgt c
//
LOCUS       DASUCJ010000416         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_875644, whole genome shotgun sequence.
ACCESSION   DASUCJ010000416 DASUCJ010000000
VERSION     DASUCJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875644"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFU90_04975"
     CDS             complement(<1..344)
                     /locus_tag="VFU90_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5479162.1"
                     /translation="MAETTYLEAIRQALFEEMDRDPNVFVLGEDIGAYGGAFKVTDGL
                     LAKYGESRVIDTPISEAAIVGAAAGAAHMGMRPVCEMQFIDFISCAYDMLTNYVATAR
                     YRAFLPCPMVVRG"
     gene            complement(337..>1001)
                     /locus_tag="VFU90_04980"
     CDS             complement(337..>1001)
                     /locus_tag="VFU90_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5479163.1"
                     /translation="RGQDRVGLVYIGDGATSTGAFHEGINFAAVQRCPLVVVVENNGY
                     AYSTPTSRQTAAAEFTDKALAYGIPGEQADGNDVQATYDVTKRAVDRARRGEGVTLIE
                     LLTYRRKGHAEHDNQSYVPEGEIARWASTNDPVDRYVAHLESAEGVSRGELTEIDARV
                     QREIDTATDLAEQSPMPEPSDALVGVYANPPSAEAPWYRQGVSSAVTANERPAAWGTF
                     NG"
BASE COUNT          153 a          359 c          311 g          178 t
ORIGIN      
        1 ccgcgcacca ccatgggaca ggggagaaag gcgcggtagc gcgccgtcgc cacgtaattg
       61 gtgagcatgt cgtaggcgca cgagatgaag tcgatgaact gcatctcgca caccgggcgc
      121 atgcccatgt gcgcggcgcc cgccgcggcg cccacgatgg cggcttcgga aatcggcgtg
      181 tcgatcaccc gcgactcgcc gtacttcgcg agcagtccat ccgtcacctt gaacgcgccg
      241 ccgtaggcgc cgatgtcttc gcctaacaca aagacgttcg ggtcgcggtc catttcctcg
      301 aacagcgcct ggcggatcgc ttcgagatac gtcgtctcag ccattgaagg tcccccaggc
      361 agccggccgt tcgttcgccg tcacggcact cgacacaccc tgccggtacc acggcgcctc
      421 ggccgacggc ggattcgcgt ataccccgac gagcgcgtca ctgggttctg gcatcggcga
      481 ctgctcggcg aggtcggtgg cggtgtcgat ctcgcgttgc acgcgcgcgt cgatctccgt
      541 cagctcgccg cgcgagacgc cctccgcgct ctccaaatgc gcgacgtacc ggtccaccgg
      601 atcgttcgtc gacgcccacc gcgcgatctc cccctcggga acgtacgatt ggttgtcgtg
      661 ctccgcgtgt cccttgcggc gatacgtgag cagctcgatc aacgtcacac cctcgccgcg
      721 ccgcgcacgg tcgacggcgc gcttggtcac atcgtaggtg gcctgcacgt cgttgccatc
      781 cgcctgctcg ccgggaatgc cgtacgcgag cgctttgtcc gtgaactccg ccgccgccgt
      841 ctgtctggac gtcggcgtcg agtacgcgta gccgttgttt tcgacgacca cgactaacgg
      901 acaccgttgc accgccgcga aattgatgcc ttcgtggaac gcgccggtgg acgtcgcccc
      961 gtcgccgatg tacacgaggc ccacgcgatc ctgtccgcgc a
//
LOCUS       DASUCJ010000417         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_96363, whole genome shotgun sequence.
ACCESSION   DASUCJ010000417 DASUCJ010000000
VERSION     DASUCJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96363"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFU90_04985"
     CDS             <1..384
                     /locus_tag="VFU90_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479164.1"
                     /translation="RVTHLPGVPYEEMPDYFVAGDVFFFPSHLEGYGIVVGEAMANGM
                     PVVTTRAKGIRGVIAEGETALAGDVGDVDAHADHLRRVIQDEMLRRRLGDAGRRRIAT
                     HFAWDGIIDRLMLALEQPIRAGSAA"
     gene            381..1523
                     /locus_tag="VFU90_04990"
     CDS             381..1523
                     /locus_tag="VFU90_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479165.1"
                     /translation="MTAVLDAGAVRPLAGWRAAALDRGGLTDCSLIIPTYERPTAVTT
                     LLDALGHLADTPAEVVIVDGSRRPETADAIDAWLAAHRPRFDLAYVPSPAGLTLQRNV
                     GIEASSGAFVYYLDDDCVPFPGYFQAIRDVFARDTTGAVGAVAGAIVNNMGSALSWRW
                     RMRFALGLVRRADPGSYAQSGASLPITHVPRFTGTRRVQVVPGGAVAYRRAVFERYRF
                     SLYFSGYAQGEDLEMSLRIGRDWTLLWCGDAHVSHNHAPEGRPNAARKGRMEVRNRYF
                     IWKRHARPRASDSLRFWLDFAYGMGVDLLEFAAHPTSIAPLQHARGTIGGLLSCVIDR
                     PRYDEPPIGRGYDITFADVTRQAGYPLGGAGEIGDARAPLHSGTPS"
     gene            1520..>2988
                     /locus_tag="VFU90_04995"
     CDS             1520..>2988
                     /locus_tag="VFU90_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479166.1"
                     /translation="MTTSTILQAFEPESACAPLSVDVPVIEYVGSSAHGAPSAWMPSG
                     AGRWSSADDTLRFGLVLGTLCLDEHSALRPVTLTAVAERPASIRQCSIRLGARCVGRP
                     HHLDRYCRWREIAHRAVTNESTPFVLRWRDPSGAMLELRSIRGCVPVAVSCSDGRLTI
                     EVQLDAAVLHPRWSFAAGQAESTAAPTRAIGWSSRVELLVRRVDRPAPTPAIVGRMPF
                     GAEAALAITDHPDFDDEDRFRVFAHGGNGSRGWIGRGLRLTKSVFAVESDSTPRPPVP
                     TLERPAYCALLDELYDEGSEIAPHGVNESANIGPERFRRALAAIAQRYRPDTWIDHGL
                     TLAYCYSMGGADNPDYDLLNRLREHGISTLWSYHDAPIDASGSLNLLAPNPRDLGQST
                     RGAVRHAANRDFLIAAHYLRSAFLSRATGALHHDVGKALSVGRRAYLTRTGSQASRPA
                     AALSAAGVGLLGLFEASRQRRRGASPSDRLFSRREGLEWA"
BASE COUNT          441 a         1020 c         1007 g          520 t
ORIGIN      
        1 cgagtgacgc acctccccgg tgtgccctac gaggagatgc cggattattt cgtcgccggc
       61 gacgtgtttt tcttcccaag ccatctcgag ggatacggga tcgtcgtggg tgaagcgatg
      121 gcgaacggga tgcccgtcgt gacgacgcgt gccaagggca ttcgcggcgt catcgcggag
      181 ggcgaaacag cgcttgccgg cgatgtgggt gacgtcgacg cgcatgcaga tcatctgcgc
      241 cgcgtgattc aggatgagat gctccgccgc cgattgggcg acgccggcag acggcgcatc
      301 gcgacgcact tcgcatggga tgggatcatc gaccgcctca tgctcgcact cgagcaaccg
      361 atccgcgcgg gttcggccgc gtgactgccg tgctggatgc aggcgctgtg cggccgctgg
      421 ccggatggcg cgccgcggcg ctcgaccggg gcggcctaac ggattgctcg ctcatcatcc
      481 ccacgtacga gcggccgact gccgtcacga cgctgctcga cgcgttaggc catctcgccg
      541 acacgccggc ggaggtcgtc atcgtggatg ggtcccgtcg cccagagacc gcggacgcca
      601 tcgatgcatg gctggcggcg cacagaccgc gcttcgatct cgcgtacgtg ccgagcccgg
      661 ccggcctcac cctgcaacgc aacgttggca tcgaggcgtc cagcggggcg ttcgtgtatt
      721 acctcgacga tgattgcgtg ccgttccccg gctatttcca ggcgatccgc gatgtgtttg
      781 cgcgcgatac gaccggcgcc gtcggcgccg tggctggggc catcgtcaac aacatgggga
      841 gtgcgctatc gtggcgatgg cgcatgcggt tcgcgctggg tctcgtccgc cgagccgatc
      901 cggggtctta tgcgcagagt ggcgcgtccc ttcccatcac ccacgtgccg cgctttaccg
      961 gtacacgtcg cgtgcaggtc gtccccggcg gcgcagtcgc ctatcgccgt gccgtattcg
     1021 agcggtatcg cttctcgctc tacttcagcg ggtacgcgca gggcgaggat ctcgagatgt
     1081 cgcttcgcat cgggcgcgat tggacccttc tctggtgcgg cgatgcccac gtatcacaca
     1141 accatgcgcc ggaagggcgt cctaacgccg ccaggaaggg ccgcatggaa gtgcgcaatc
     1201 gatacttcat ctggaagcgg catgcgaggc cgagggcgtc cgactcgctc agattctggc
     1261 tcgactttgc ttacgggatg ggtgtggacc tgctcgagtt cgcggcgcac cccacctcga
     1321 tcgcgccgct ccagcacgcg cgcgggacga tcggcggatt gctcagctgc gtgatcgacc
     1381 gcccgcgata cgacgaaccg cccattggcc gcggctacga catcaccttc gccgatgtca
     1441 ctcggcaggc cggctatccg ttaggcggtg ccggcgagat cggcgatgcc cgtgcaccgc
     1501 tgcactcggg cactccgtcg tgaccacgtc gacgatcctc caggcgttcg agcccgagag
     1561 cgcatgcgca ccgctgagcg tggatgtccc ggtgatcgaa tacgtgggat cgagtgccca
     1621 cggcgcgccg agcgcatgga tgcccagcgg cgcgggacgg tggtcgagcg ccgacgacac
     1681 gctgcgcttc ggactcgtgc tgggcacgct gtgcctggac gagcattcgg cgttgcggcc
     1741 ggtcacgctg acggcggtgg ccgaacgccc ggcgtccatt cgacagtgct cgatccggct
     1801 cggtgcgcgc tgcgtcggac ggccgcacca cctcgatcga tattgtcgct ggcgcgagat
     1861 cgcgcatcgt gcagtcacga acgaatcgac gccattcgtg ctgcgctggc gcgatccgtc
     1921 cggcgcgatg ctcgagctgc gctcgattcg cggatgtgta ccggtcgccg tgtcgtgtag
     1981 cgatggccgc ctgacgatcg aggtccagct cgacgccgcc gtgctccacc cacggtggtc
     2041 gttcgctgcg ggacaggccg agtcgacagc ggcgccgacc cgcgccatcg gctggtcgtc
     2101 acgtgtggag ctcctggtgc gtcgcgtcga ccggcccgcg ccgacgcccg cgattgtcgg
     2161 gcgcatgccg ttcggcgccg aagcagcgct ggcgatcacg gaccaccccg atttcgatga
     2221 cgaagaccga ttccgcgtgt tcgcgcacgg cgggaacggc tctcgcggct ggatcggccg
     2281 cggtctccgc ctaacgaaga gcgtgtttgc cgtggagagc gattcgactc cgcgaccacc
     2341 ggtgccgaca ctcgagcgtc ccgcctactg cgcgttgctc gacgagctgt acgacgaagg
     2401 aagcgagatc gcgccgcacg gcgtgaacga atccgcgaac attggccccg agcggttccg
     2461 gcgcgccttg gccgccatcg cccaacgcta tcgcccggac acgtggatcg accacgggct
     2521 gaccctcgcg tactgctatt cgatgggcgg cgccgacaat ccggactacg acctcctgaa
     2581 ccgtctgcgt gagcacggca tcagcacact ctggtcctac cacgacgcgc caatcgatgc
     2641 gtccggatcg ctcaaccttc tcgcgccgaa tccgcgcgac ttgggtcagt cgacacgcgg
     2701 cgcggtgcgt cacgccgcga accgcgattt tctcatcgct gcgcattatt tgcgctcggc
     2761 ctttctctcg agggcgaccg gcgccctaca tcacgacgtc ggtaaggcgc tgagcgtcgg
     2821 ccgccgtgcg tatctcacgc gcaccggatc gcaggccagc cgccctgccg ccgcactgtc
     2881 cgccgcgggc gtcgggcttc tcggactgtt cgaggcgtcg aggcagagac gtcgcggcgc
     2941 ttcgccctcc gaccgcttgt tcagccggcg cgaagggctc gagtgggc
//
LOCUS       DASUCJ010000418         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1502293, whole genome shotgun sequence.
ACCESSION   DASUCJ010000418 DASUCJ010000000
VERSION     DASUCJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502293"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1288
                     /locus_tag="VFU90_05000"
     CDS             101..1288
                     /locus_tag="VFU90_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479167.1"
                     /translation="MNKALVFAAALCMPPLLAACGNGGASEPPVTSVDPAASSRLQFA
                     VGVATIAFNGGQSIAYGLNTVLTLRQSNGLSGTLYNVPQIIGPSSFNVLISTQTGNEV
                     LSAGSDLGTNHITWGTLNQTLWTGPPRGLKAATTGAFGYGFCPCNSDAGPVNGTAPLY
                     QAFNLPVYGNNEERFYGGPPAFPAADPSVAALGFQGYSLGFTDFAVKPVLGAYRLYAA
                     VPPAYTTPQNPTPSPNPDGTPTPAPGVLAATAQLTKLAPLPAFSTPSFTADGKGGGAV
                     RLQVPAGVSEAAVIVRAIGGSGVGVCVQSHPVDSFSTLIARHAGTQRLVLPDKAGPAT
                     GSGQPSATICPKGSYQIYAVGADYPLYEASYPANLSQLPRIKGPSGQADVTTSDILNG
                     VYP"
     gene            1285..>2192
                     /locus_tag="VFU90_05005"
     CDS             1285..>2192
                     /locus_tag="VFU90_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479168.1"
                     /translation="MSAPPVRSFVARAFAIAASAVLAACSGGPGTPDGPAVNSPGGGA
                     PPPTPLVGVLLTVTVPPPSHGLGPDYVSPNMQSLSIGLASVDGSPVSGVGASVVNTFS
                     GAHGCKLRDGARICSAKIYGSPGDDVFALTTYAGRNAGGAVLSVGTAESHVSPGGGGV
                     SVNTLSLSIDGVIASLKLGVSPREVPRGKIARAAVSLRAYDASGAQIVGGSPFQAPIL
                     LAIQGDVDRAFILHTQHASGETLRIVKPPGDITLAYDGDANASPITLQATVEGPSDPS
                     ATARFSLRGNPPPPPVGTIFALNLGAA"
BASE COUNT          340 a          799 c          689 g          364 t
ORIGIN      
        1 agtacgcacc gcccgacgtt tcggcacacg gcatcgaccc gtacctcacg tatccgaacg
       61 agccgccgat ctcgttccgc ttctactacc aggtgacgat atgaacaaag cgctcgtttt
      121 tgcggccgcg ctctgcatgc cgccgctatt ggccgcgtgc gggaacggcg gcgcgtcgga
      181 acccccggtg accagcgtcg atcccgccgc ctcgagccgg ctgcagtttg ccgtcggcgt
      241 cgccaccatc gcgttcaacg gcggccaaag catcgcgtac ggactgaaca ccgtgctgac
      301 gctgcgtcag tccaacggcc tttcgggaac gctctacaac gttccgcaaa tcatcgggcc
      361 gtcgagcttc aacgtgctga tctccacgca gaccggcaac gaagtgctct ccgccggatc
      421 cgacctcgga acgaatcaca tcacctgggg gacgctcaac caaacgttgt ggacgggccc
      481 gccgcggggg ctcaaggccg cgaccaccgg ggcgtttggc tacggtttct gcccgtgcaa
      541 ctccgacgcc ggacccgtca acggcacggc gccgctgtac caagctttca accttcccgt
      601 ttacggcaac aacgaagagc gcttctacgg cggtccgccc gcgtttccag cagcggatcc
      661 gtcggttgcc gcgctcggat tccaagggta ctcgctcggc tttaccgact tcgcggtaaa
      721 gccggtactc ggcgcgtacc ggctgtatgc ggcggtgccg ccggcctata cgacgccgca
      781 aaaccccacc ccgagcccca atccggacgg gacgccgacg ccggctcccg gcgttcttgc
      841 ggctaccgcg cagctgacga agctcgcgcc gctgccggcc ttttccacgc cgtcgtttac
      901 cgccgacggc aagggcggcg gcgcggtgcg cttgcaagtt ccggccggcg tcagcgaagc
      961 cgccgtcatc gtacgcgcga tcggcggcag cggggtcggc gtctgcgtgc agtcgcatcc
     1021 ggtcgatagc ttctcgacgc tgatcgcacg gcacgccggt acgcagcgtc tcgtgctgcc
     1081 cgataaggcc ggccccgcaa ccggatccgg acagccttcc gcgacgatct gtcccaaagg
     1141 cagttatcag atctacgccg tcggcgccga ctacccgctg tacgaggcct cgtatccggc
     1201 caacctctcg cagttgccgc ggattaaagg accgagcgga caagccgacg tcacgacctc
     1261 ggatattctc aacggagtct atccgtgagc gcgccgccgg ttcgctcgtt cgtggcgcgc
     1321 gcgtttgcga tcgcggcatc ggccgttctc gcggcttgca gcggcggtcc gggaaccccc
     1381 gacggtccgg cggtcaactc gccgggcggc ggcgcacccc cgcccacccc gctcgtcggc
     1441 gtcttgctga cggtcacggt cccgcctccg tcgcacggtc tcgggcccga ctacgtctcg
     1501 cccaacatgc aatcgctctc gatcggtctc gcatcggtcg acggttcgcc cgtttccggc
     1561 gtcggcgcga gcgtggtgaa cacgttttcg ggcgcgcacg gatgcaagct gcgcgacggc
     1621 gcgcggattt gcagcgcgaa gatctacggt tccccgggcg acgacgtctt cgccctcacg
     1681 acgtatgcgg gccgcaacgc gggcggagcg gtgctctcgg tcggaacggc cgagtcgcac
     1741 gtctcccccg gcggcggcgg ggtcagcgta aacaccctgt cgctgtcgat cgacggcgtg
     1801 atcgcatcgc tgaagctcgg cgtttcgccg cgggaagtac cgcgcggaaa aatcgcgcgc
     1861 gcggccgtat cgctgcgcgc ctacgacgcg agcggcgcgc agatcgtcgg tgggagccct
     1921 tttcaggcgc cgatcttgct tgcgatccaa ggcgacgtcg atcgcgcctt catcctccat
     1981 acccagcacg cttccggcga gacgctccga atcgtgaagc cgcccggcga catcacgctc
     2041 gcctacgacg gcgacgccaa cgcgtcaccg ataacgctgc aagccaccgt cgaggggccg
     2101 agcgatccgt cggccacggc gcggttttcg ttgcgcggca acccgccgcc gccgccggtc
     2161 ggcacgattt tcgcactcaa cctcggagcg gc
//
LOCUS       DASUCJ010000419         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1043775, whole genome shotgun sequence.
ACCESSION   DASUCJ010000419 DASUCJ010000000
VERSION     DASUCJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043775"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFU90_05010"
     CDS             <1..702
                     /locus_tag="VFU90_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479169.1"
                     /translation="GTARLTSDPVAPEQLTNDIARLFDALAGGGIGLVPLDVAYAVIA
                     TTPSGIRRIFEVKRRSYEKPSGMFGNWRMSRDIHLMDDRRHAMVREIIEQERIPFSVV
                     APFRADHPLFGAVDPFVLQNSSKGGTLDMLLNAGQFHDAIAEGSLACGVAVFGSSANL
                     SLTGSKYRYADVDQPIREAAAIHFDYGQSKYAHKDGLSSTIIDFGDFSVIRVGHCFER
                     LKSAFATRFGVTLKS"
     gene            719..1363
                     /locus_tag="VFU90_05015"
     CDS             719..1363
                     /locus_tag="VFU90_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5479170.1"
                     /translation="MSDTYKDIGFDSNRIGFGRKAGIVVVDFQKGFTDGRFPLGGRPM
                     VDRAVNNTATLLAAARKAGLPIASCYTAYTSARDAPLWKIPAVVNDFHHGHPSTELDE
                     RIYDKSYDMIVCKTAPSIFFNTAVASYFVKEGVDTVIVTGCNTSGCVRASINDAFSCG
                     FRVIVPEPCCGDVEEGPHRDNLRDVGRRYADVVGLDEVLAYIETAGRRNANEAS"
     gene            1360..>1543
                     /locus_tag="VFU90_05020"
     CDS             1360..>1543
                     /locus_tag="VFU90_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEU5479171.1"
                     /translation="MTAQPLVADPGAIDEIAAAVARVDRRMDATLARTAIVRYDAYET
                     AGGRQSASVALLDANRS"
BASE COUNT          271 a          514 c          469 g          289 t
ORIGIN      
        1 ggaacggctc ggctcacgtc ggatccggtt gcgccggagc aactgacgaa cgatatcgcg
       61 cgcttattcg acgctctcgc cggcggcggc atcggcctgg tgccgctgga cgttgcctat
      121 gccgtgattg ccaccacgcc gagcggcatc cgccgcatct tcgaggtcaa gcggcgctcg
      181 tatgagaagc cctccggcat gttcggcaac tggcggatga gccgcgatat ccatctgatg
      241 gatgatcggc ggcatgccat ggtccgcgag atcatcgagc aagagagaat cccgttctcg
      301 gtcgtggcgc cgtttcgcgc cgatcatccg ctgttcggtg cggtcgatcc gttcgtgctg
      361 cagaactcat cgaaaggcgg cacgctcgac atgctgctca atgccggcca gttccatgac
      421 gcgatcgccg aaggctcgct tgcctgcggc gtcgcggtgt tcggctcgtc cgcgaacttg
      481 tcgctcaccg gcagcaagta ccgctacgcg gatgtcgatc agcccatccg cgaggctgcc
      541 gccatccatt tcgactacgg ccagtcgaaa tacgcgcaca aggatgggct atcgagcacc
      601 atcatcgact ttggcgattt ctccgtgatc cgcgtcggac actgcttcga gcggctgaaa
      661 agcgccttcg cgacgcgttt tggcgtcacc ttgaaaagct agctttggga ctttgcacat
      721 gagcgacacc tacaaggata tcggcttcga ctccaaccgc atcggcttcg gccgcaaagc
      781 cggtatcgtc gtggtcgatt tccagaaggg ctttaccgac ggccgttttc ccctcggagg
      841 ccgtccgatg gtcgatcgcg ccgtcaacaa caccgcgaca ctgcttgcgg ccgcccgcaa
      901 ggcaggtttg ccgatcgcgt cctgctacac ggcctatacc agcgcgcgcg acgcgccgct
      961 ttggaagatc ccggcggtgg tcaacgactt tcatcacggc catccttcga ccgagctcga
     1021 cgagcgcatc tacgacaaaa gctacgacat gatcgtatgc aagaccgcgc cctcgatctt
     1081 cttcaacacg gcggtcgcct cctacttcgt caaagaaggc gtcgacaccg tgatcgtcac
     1141 cggctgcaac acgtcgggct gcgtgcgcgc cagcatcaat gacgcctttt cctgcggctt
     1201 ccgcgtcatc gtgccggagc cctgctgcgg cgacgtggag gaaggcccgc accgcgacaa
     1261 tctgcgcgac gtcggacgcc gttacgccga tgtggtcggc ctcgacgaag tgctcgccta
     1321 tatcgagacg gccgggcgcc gcaatgcgaa cgaagcgtcg tgaccgcgca gcccctcgtt
     1381 gccgatccgg gcgcgatcga cgagatcgcc gccgcggttg cccgtgtcga caggcgaatg
     1441 gacgccacat tggcgcggac ggcaatcgtc cgctatgacg cctacgagac tgcgggcggg
     1501 cgtcaatccg cgtccgtggc gctgctcgac gcgaaccgct ccg
//
LOCUS       DASUCJ010000420         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_478409, whole genome shotgun sequence.
ACCESSION   DASUCJ010000420 DASUCJ010000000
VERSION     DASUCJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478409"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..>1218)
                     /locus_tag="VFU90_05025"
     CDS             complement(121..>1218)
                     /locus_tag="VFU90_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5479172.1"
                     /translation="LRAPAPADKMLGRTWANFSFRRGQLPMANRSIAVTALAEMTHGQ
                     EADLFALLTVKEELTTRDGKPYFKVGFRDARREVSFPIWGDSPWAEECRRQWTPGVAY
                     KLRAVFRETNYGPQLDIRKIREVVDADTADGYDPAMSLPQSRFDPAAMFDELLAIARD
                     EIADAALRRLTVSLLEENRTALLALPAATRNHHAFAGGFLEHVLSATRTAVFLAKKYD
                     DYYTDLEPRLNVSLVVAGAILHDIGKLRELEWKPEGAAYTSAGALIGHILQGRDLVRE
                     AAARQAAAGEPLDADVLLRLEHVIVSHQRLPEWGSPKPPMTPEALLVHYADDLDAKFQ
                     MVYLALRDDATPGPLTSKKNVLVQQFYRGEL"
BASE COUNT          212 a          383 c          409 g          214 t
ORIGIN      
        1 tcgtcgtaga cattcgtcac ctggcagtgg accaacaaag gaattctatc cagatttgag
       61 gtcatcgtca cgaggcggaa aactccagcc atacgaagat ccgcagaacg cattgtttcg
      121 ctataactcg ccccggtaaa actgctgcac gagcacgttc ttcttcgacg tgagcgggcc
      181 gggcgtggcg tcgtcgcgca gggcgagata gaccatctga aacttggcgt cgagatcgtc
      241 ggcgtagtgg acgagcaacg cctcgggcgt catcggcggc ttgggcgatc cccactcggg
      301 cagccgctgg tgcgagacga tgacgtgttc cagacgcaac agcacgtcgg cgtcgagcgg
      361 ctccccggcc gcggcttgcc gcgctgcggc ttcgcggacc aggtcgcggc cctggaggat
      421 gtggccgatg agggcgccgg cgctcgtgta agcggcgccc tcgggcttcc actccagttc
      481 gcggagcttg ccgatgtcgt gcagaatggc gccggcgacg acgagagata cgttcagccg
      541 cggctcgaga tcggtgtagt aatcgtcgta cttcttggcc agaaagacgg ccgtccgcgt
      601 ggcgctcagc acgtgctcca aaaagccgcc ggcgaaggcg tgatgattgc gcgtcgccgc
      661 cggcagcgcg agcaatgcgg tgcggttttc ctcgagcaga gaaaccgtca gccggcgaag
      721 cgcggcatcg gcgatttcgt cgcgggcgat cgccaacagc tcgtcgaaca tggccgccgg
      781 gtcgaaccgc gattgcggca gggacatcgc cgggtcgtag ccgtcggccg tatcggcgtc
      841 gacgacctcg cggattttgc ggatgtcgag ctgcggcccg tagttcgtct cgcgaaacac
      901 ggcccgcagc ttgtaggcca cgccgggcgt ccactgccgg cggcattctt cggcccaggg
      961 cgaatcgccc cagatcggaa agctgacttc gcggcgggcg tcgcgaaagc cgactttgaa
     1021 gtacggcttg ccgtcgcggg tggtcagctc ttccttgacc gtcagcagcg cgaacagatc
     1081 ggcctcctgg ccgtgcgtca tttccgccag cgccgtcacg gcgatcgagc ggttggccat
     1141 cggaagttgc cctcgtcgaa aggaaaagtt cgcccaggtt cgcccaagca tcttatcggc
     1201 cggcgccggc gctcgcaa
//
LOCUS       DASUCJ010000421         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_340765, whole genome shotgun sequence.
ACCESSION   DASUCJ010000421 DASUCJ010000000
VERSION     DASUCJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340765"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..850)
                     /locus_tag="VFU90_05030"
     CDS             complement(152..850)
                     /locus_tag="VFU90_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479173.1"
                     /translation="MKLSLTAAVAGALLLAACSGGTTLNQAPASGVAVNASNSGVPFA
                     ANSRLAAAPILAAAGAAGEADSDRVSPDETKAAAYCKQTGGTVYMRRAEYDTNGSKPL
                     VFAGRKGFCQYTATSDGSRIHILLSTLYAVKPSLAALAYILKPPTGQCNGNPASCYCT
                     LLGGSDQFGGASGAGGSWVNNKSEDVDLEACVFPDMSSIDSWGLAYNANGIIRGIDLT
                     TVMRYKYPSNLKVR"
     gene            complement(918..>1804)
                     /locus_tag="VFU90_05035"
     CDS             complement(918..>1804)
                     /locus_tag="VFU90_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479174.1"
                     /translation="SANTWSSTILAHGLPALGLLASVALRASPFALLALLAAVAAPFG
                     LRDDRRLGWAAFAAVVLFLAMPLAYADTMPQLATGASLRYAAPSIALGALLLAPWAGR
                     LPRVATAMLVLSSAFGAAVVLAIFWNDAPTRIAMAIALLAAGNVALARRLRAPWQIPA
                     GLGAVIVVTWMLAARYPLAYYTDALRVNGRATAVYGWIARTQPRAIGGWGLKLGAINA
                     LAPATRTLDLPESDPCAAARAHGVLLVAVAESTRSTAFDAARLNAARACGRVRFDDGL
                     AVVAGDFGAPAAARLGKP"
BASE COUNT          297 a          598 c          634 g          275 t
ORIGIN      
        1 agtcatgact cgtttccgcc ttcgggccgt ttttgcccgg atacttcgtt gctcatcggt
       61 cacgaagtga cggcttcgct ccctcttcgc gcctcgtctc cgaacaaaac tcggccccga
      121 atccgaaaac ggccataact ccggcggtct cctagcgaac tttcagattg ctgggatact
      181 tgtagcgcat caccgtcgtg aggtcgatgc cgcggatgat cccgttggcg ttgtaggcga
      241 gtccccacga gtcgatcgac gacatgtcgg ggaaaacgca cgcctcgaga tcgacgtctt
      301 cggacttgtt gttgacccag cttcccccgg cgccgctcgc gccgccgaac tggtcggatc
      361 cgccgagcag ggtgcagtag cacgatgccg ggttaccgtt gcactgaccg gtcggaggct
      421 tgaggatgta cgcgagcgcc gccagcgacg gtttcacggc gtacagcgtc gagagcagga
      481 tgtggatgcg cgacccgtcg gacgtcgcag tgtactggca gaagcctttg cggccggcga
      541 aaaccagcgg cttcgagccg ttggtgtcgt actccgcgcg ccgcatgtag acggttccgc
      601 cggtctgctt gcagtaggcg gcggctttgg tttcgtccgg gctcacccga tccgagtccg
      661 cctctccggc agcaccggcg gcggccagaa ttggggcggc ggcgagccgg ctattcgccg
      721 cgaacggaac gcctgagttg gaggcgttga cggcgacgcc cgacgcgggc gcctggttga
      781 gcgtggtccc gccgctgcat gcggcaagca aaagcgcgcc ggcgacggcg gcggtcaacg
      841 aaagcttcat gtcgttcccc caaaaaagtc aaattgcgct ccgaaaacgg tgcgcacacg
      901 tgtagtaccg attcgtgcta gggtttacct aggcgtgcag ccgccggcgc gccgaaatct
      961 ccggcgacga ccgcgagtcc gtcgtcgaag cgtacgcgcc cgcacgcgcg cgcggcgttc
     1021 aaacgagcgg cgtcgaacgc cgtcgagcgt gtactctcgg caaccgctac gagcagcacg
     1081 ccgtgggcgc gtgcggccgc acacgggtcg ctttcgggca ggtcgagggt gcgcgtggcc
     1141 ggcgcgagcg cgttgattgc gccgagcttc aacccccaac cgccgatcgc gcgcggctgc
     1201 gtgcgcgcga tccacccgta tacggcggtg gcgcggccgt tgacgcgcag cgcgtccgtg
     1261 taatacgcca gcggatagcg tgcggcgagc atccacgtga cgacgatgac ggcgcccagt
     1321 cccgccggga tttgccaggg tgcgcgcagc cggcgcgcca gcgctacgtt gccggcggcg
     1381 agcagcgcga tcgccatcgc gatgcgcgtc ggcgcgtcgt tccagaagat cgcgagcacg
     1441 acggctgcgc cgaaggcgct cgagagcacg agcatcgcgg ttgcgacgcg ggggaggcgg
     1501 ccggcccacg gtgcgagcag caacgcgccc agtgcgatcg acggtgcggc gtagcgcagg
     1561 gacgcgccgg tggcgagttg cggcatcgtg tcggcgtagg ccagcggcat cgcgagaaac
     1621 agcacgaccg ctgcgaacgc ggcccacccg agccgccggt cgtcgcgcag cccaaacgga
     1681 gcggcgacgg cggcgagcaa cgccagcaac gcgaacggcg acgcgcgcag cgcgacgctc
     1741 gcgaggagcc cgagcgccgg cagtccgtgc gcgagaatcg tcgagctcca cgtattcgcc
     1801 gacg
//
LOCUS       DASUCJ010000422         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_615807, whole genome shotgun sequence.
ACCESSION   DASUCJ010000422 DASUCJ010000000
VERSION     DASUCJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615807"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFU90_05040"
     CDS             complement(<1..945)
                     /locus_tag="VFU90_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5479175.1"
                     /translation="MLADGVGAADERTRSGLRIGEWSVDRQTNELRRGSEAVRIEPKA
                     MDVLMLLAEHVGRVVSREQLFAAVWPGVVVGDDALTQSIIKLRRVLGDDPRSPAYIET
                     ISKRGYRLIAPVRQGEAPDSAPRLDAAASSKPALPVRRHALRIALLAGVGLAIAAGAA
                     YYYYARHSEPAAEAAVDAASVRPSDWVTITVLPFEALGPDGDHAYLARGISDTLVTDL
                     SRLSDLRVIQPSGAANESTAGQARYRVSGSVQRDSGALRINVRLTDTRTGEQLWSGRF
                     ERPFGDLFSIQDEIIARLVELLPARISDAERERLAKRYT"
BASE COUNT          164 a          341 c          356 g          164 t
ORIGIN      
        1 cgtgtaccgc ttggcgagcc gctcccgctc cgcatcgctg atcctggccg gcagcaattc
       61 gaccagccgg gcgatgattt cgtcctggat cgagaagaga tcgccgaacg ggcgctcgaa
      121 tcgtcccgac cagagttgct cgccggttct ggtgtcggtg agacggacgt tgatccgcaa
      181 agcgcccgaa tcgcgttgca cgctgccgga aacgcggtag cgtgcctgtc ctgccgtcga
      241 ttcgttcgct gcgccggacg gctggatcac ccgcaagtcg gagagacgcg aaagatcggt
      301 taccagggtg tcgctgatgc cgcgggcgag gtaggcgtgg tcgccgtccg ggcccagggc
      361 ttcgaacggc agcaccgtga tggtgaccca gtcggacggg cggacgctcg ccgcgtcgac
      421 tgccgcctct gcggcgggtt cggagtgccg cgcgtagtag taataggcgg cgcccgcagc
      481 gatggccagc ccgacgccgg cgagcagcgc gatccgaagg gcgtgacggc ggacggggag
      541 cgcaggcttg gatgacgccg cagcatcgag gcgcggcgcg ctatccggtg cctccccctg
      601 ccgcaccggc gcaatcaagc ggtagccgcg cttcgaaatg gtttcgatgt aggccgggga
      661 gcgcggatcg tcgcccagca cccggcgcag cttgatgatg ctttgagtga gggcatcgtc
      721 cccgacgacg acgcccggcc agacggccgc aaagagctgc tcccggctga ccacccgtcc
      781 gacgtgctcg gcaagcagca tcaggacgtc catcgccttc ggttcgatgc gcaccgcctc
      841 gctgccgcgg cgcagctcgt tggtctggcg atcgaccgac cactcgccga tccgaaggcc
      901 cgatcgggtt cgttcgtcgg cggcgccgac gccatctgcc agcattgcaa ggtgcctcga
      961 cgtccgcatg caacgcgttg atcacgcggc tcgaatgata gcgtaggaag accatcgccg
     1021 ttccg
//
LOCUS       DASUCJ010000423         1358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_585363, whole genome shotgun sequence.
ACCESSION   DASUCJ010000423 DASUCJ010000000
VERSION     DASUCJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585363"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="ugpB"
                     /locus_tag="VFU90_05045"
     CDS             complement(<1..1016)
                     /gene="ugpB"
                     /locus_tag="VFU90_05045"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEU5479176.1"
                     /translation="MAYRFKRLACVASAIVFACAATAAQAVVEIHWWHSMTGALSDKV
                     NEIANKFNASQSDYKVIPVYKGQYDESMTAAIAAYRAGDPPQIVQVSEVGTATMMAAK
                     GAVKPVYQLMAEQHQPFDPKSFLGAVSSYYSDTSGHLISMPFNSSTQVLYVNDDAFKK
                     AGLDPANPPKTWPELEKDAEKLKAAGVPCEFTTGWQSWVQLESFSAWHNVPFASLQNG
                     FGGLGTRLEFNGPVQVRHIQNLADWAKKGLFTYKGRKDEPLAAFTSGECAMITTSSGS
                     YANIKANAKFAWRVATLPYYPDVKGAPQNTIIGGATLWVLTQKNRDVYPGIARFFTFL
                     SSPE"
BASE COUNT          220 a          446 c          459 g          233 t
ORIGIN      
        1 atctccggcg acgacaggaa cgtgaagaac ctggcgatgc cggggtagac gtccctgttc
       61 ttctgcgtca gcacccacag cgtcgcgccg ccgatgatcg tgttctgcgg cgcacccttg
      121 acgtcggggt agtagggcag cgtcgcgacg cgccaggcga acttcgcgtt ggccttgatg
      181 ttcgcgtacg agcccgacga cgtcgtgatc atcgcgcact cgccgctcgt gaacgcagcg
      241 agcggctcgt ccttgcgccc cttgtaggtg aagaggccct tcttcgccca gtcggcgaga
      301 ttctggatgt ggcgcacctg gacggggccg ttgaactcga gcctggtgcc gaggccgccg
      361 aagccgttct gcagcgacgc gaacggcacg ttgtgccacg cgctgaagct ttcgagctgc
      421 acccatgact gccagccggt ggtgaactcg cagggcacgc cggcggcctt cagcttctcg
      481 gcgtccttct cgagctcggg ccacgtcttc ggcggattgg cgggatcgag cccggccttc
      541 ttgaacgcgt cgtcgttgac gtacagcacc tgcgtcgagc tgttgaacgg catcgagatc
      601 aggtggccgg aggtatccga gtagtacgac gacacggcgc cgaggaacga cttcgggtcg
      661 aacggctggt gctgctcggc catcagctgg tataccggct tcacggcacc cttcgccgcc
      721 atcatcgtcg ccgtgccgac ctcggacacc tggacgatct gcggtggatc gccggcgcga
      781 taggcggcga tcgccgccgt catcgactcg tcgtattgcc ccttgtagac cggaatcacc
      841 ttgtagtcgg actgcgatgc attgaacttg ttggcgatct cgttgacctt gtcactcagc
      901 gcccccgtca tcgagtgcca ccagtgaatc tcgacgaccg cctgggccgc cgtcgccgcg
      961 cacgcgaaga cgatcgccga cgcgacgcac gcaagacgtt tgaagcgata tgccatgttg
     1021 tcctcctgaa ggaaccccct gcgggcccgg gggcccgtcc gggttgcgat tctagtgcaa
     1081 aacgactgcg gcgtgcggcg gccgcacgga cgtcggcgta cgacgcatcg cgggcgcgcg
     1141 aacgcctacg tgcggcgtgc gccgggtgcc agcgtcaccg cgagaacgag ccagccggcg
     1201 aggaatgcca cgccgccgac cggggtgagg tatgcccagc cgcgcgctgc gccgagtgcg
     1261 atgagctaga ggcttccgga gaagaggacg atcccgacga cgagcagcgt cgtaccgagc
     1321 gcgaggccgc gtcgggcacg cgcggtgcgc tgcagcac
//
LOCUS       DASUCJ010000424         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_401545, whole genome shotgun sequence.
ACCESSION   DASUCJ010000424 DASUCJ010000000
VERSION     DASUCJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401545"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFU90_05050"
     CDS             <1..256
                     /locus_tag="VFU90_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5479177.1"
                     /translation="SAALGIAYAAGGRVDLFTHTNVYPWDIAAGILFVREAGGAASDR
                     AGGPLRITSRAFAAGGRRVHDDFIARYSSMQGGVPGATPG"
     gene            272..946
                     /locus_tag="VFU90_05055"
     CDS             272..946
                     /locus_tag="VFU90_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5479178.1"
                     /translation="MNPRLLVVAAGCTFAIALTACSGGSSKPKASPTPSTPSAVDVIA
                     QWVHDNRNVDFVGDCDNAKQGVDIGKLCVRIAGRRGTLWAYNLGPTFSDPTALAMLQQ
                     QKDGTWKVLSVTNRDPSAGDVPGIAWPLEIGDQVVVVGLAQGDCLNVRDTPSTKGKQI
                     ACVPQGTKAIIQDGPQQGESYTWWKVSGDAGTGGFAGWAADKWMRLPEAIAQALQPQA
                     TPTPGG"
     gene            1090..1647
                     /locus_tag="VFU90_05060"
     CDS             1090..1647
                     /locus_tag="VFU90_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU5479179.1"
                     /translation="MQRGDRAGDEASIAFEHRFRRQNYCLRVWRRGRSLHLRGIPWLP
                     QAVRVLCQVVFHADIPVQVDIPRGVVFMHNGLGTVIHTRVRFAGPAIVFHNVTIGNRT
                     TADDRVPTIGRRVFIGAGAFIIGDIEIGDDCIIGANSVVTASVPSGHRAVGSPARISR
                     CADGVIDELFPGVPASTPGPRRAAA"
     gene            1730..1805
                     /locus_tag="VFU90_05065"
     tRNA            1730..1805
                     /locus_tag="VFU90_05065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1763..1765,aa:Gly,seq:tcc)
BASE COUNT          296 a          645 c          690 g          298 t
ORIGIN      
        1 cagcgccgcg ctcggcatcg cgtacgccgc tggaggtaga gtcgacttgt tcacgcacac
       61 aaacgtgtat ccatgggaca ttgccgctgg tattctattc gtgcgcgaag cgggcggagc
      121 cgcatcggat cgcgcgggcg ggccgttgcg gatcaccagc cgggcgtttg cagcaggcgg
      181 gcgccgcgtg cacgacgact tcatcgcgcg ctactcatcg atgcagggcg gggtaccggg
      241 cgcgacgccc ggatgacagg gaggatgtgt catgaatccg cgcttgctcg tcgtcgccgc
      301 cggctgcacg ttcgcgatcg cgctcaccgc gtgctcgggc ggctcgagca agcccaaggc
      361 gagcccgacg ccgtcgacgc cgagcgcggt cgatgtcatc gcgcaatggg tgcacgacaa
      421 ccgtaacgtc gacttcgtcg gtgactgcga caacgcgaag cagggcgtgg acatcggcaa
      481 gctctgcgtc cgtatcgcgg gccgtcgcgg cacgctgtgg gcctacaacc tcggtccgac
      541 gttctcggac ccgacggcgc tggcgatgct gcagcagcag aaggacggca cgtggaaggt
      601 gctgtcggtg acgaaccgcg acccgagcgc tggggacgtg ccggggatcg cctggccgct
      661 cgaaatcggc gaccaggtcg tcgtcgtcgg gctggcgcaa ggcgactgcc tgaacgtccg
      721 tgacacgccc tcgacgaagg ggaagcagat cgcgtgcgtg ccacaaggga cgaaggcgat
      781 catccaggac gggccgcagc agggcgagag ctacacgtgg tggaaggtgt ccggcgatgc
      841 gggcaccggc ggcttcgccg gctgggctgc cgacaagtgg atgcgactcc cggaggcgat
      901 cgcgcaggcg ctccagccgc aagcgacgcc gacgcctggc gggtagtcgc aggttccacg
      961 tcgcaggccg cgtgtacggt tgggcgcggc cggagcgtcg cgcacggtcg accgaatcgc
     1021 cggcggcagt ctcgcatcag cgggtgatcc ccgataccgt gcccggcgcc cggcggagac
     1081 actgaccgcg tgcaacgagg agatcgcgcg ggcgacgagg cgagcatcgc cttcgaacac
     1141 cggtttcgcc gccagaacta ctgcctgcgg gtgtggcgcc ggggccggtc gctccatctg
     1201 aggggtatcc cgtggcttcc gcaggccgtg cgcgtgctgt gccaggtcgt cttccacgca
     1261 gacatcccgg tgcaggttga catcccgagg ggcgtcgtct tcatgcacaa cgggctgggc
     1321 accgtgatcc acacgcgcgt acggttcgcc ggtccggcga tcgtcttcca caacgtgacc
     1381 atcggcaacc ggacgacggc cgacgatcgc gtgccgacga tcgggcggcg cgtcttcatc
     1441 ggcgcgggtg cgttcatcat cggcgacatc gagatcggcg acgactgcat catcggggcg
     1501 aacagcgtcg tgacggcgag cgtgccctcc gggcaccgcg ccgtcggcag cccggcgcgg
     1561 atctcgcgtt gcgccgacgg ggtcatcgac gaactgttcc ccggcgtccc ggcatcgacg
     1621 cccggcccgc gtcgcgcggc cgcctgagcc gaagctgcgt gtccggtgca ggcggcgccg
     1681 gcgccacctc ttcccgcagg tgctttcgat ataatcgtgt cgcgcacgtg cgggggtagc
     1741 tcaattggta gagcgctagc cttccaagct ggatgttgcg ggttcgagac ccgtcccccg
     1801 ctccactttg ctctcggcct acggaatttc ccgcgcgtgc tggaaagccc gcgcgacaga
     1861 ctgcactcct cagccgcgcg gccgccgtct gcgatacgct gcgtcaactg ctggtcggcg
     1921 accggccga
//
LOCUS       DASUCJ010000425         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_142398, whole genome shotgun sequence.
ACCESSION   DASUCJ010000425 DASUCJ010000000
VERSION     DASUCJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142398"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VFU90_05070"
     CDS             <1..1467
                     /locus_tag="VFU90_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5479180.1"
                     /translation="PFAQLAKEHFADKGDLRKYLMNMIYVGALARLLEIPAEAIEEGL
                     KAQFRTKPAAVESNMKAVLVGFDYASEHCEKRDPYRLEKMSGKTDGMLFMDGNRAAAL
                     GCVMGGCTVAAWYPITPSSSLCEYFIQYCERYRVDKESGEHKFAILQAEDELAAAGIV
                     FGAGWAGARAMTSASGPGISLMAEYAGYGYFAEVPGVIFDVQRAGPSTGLPTRTMQGD
                     VAFAYTLSHGDTKHVVLLPATVREAYEFAMEAFDLADRFQTPVFVLSDLDLGMNSWLS
                     PPLPYPEKSFDRGKVLTAEDLNRVPSWGRYRDVDKDGIPYRTLPGTKHANAGFFTRGT
                     GHDEEARYSELPEVWKRNLDRLVRKHETARTAVPAPVVSGKGHRIAILAYGTTHHAVV
                     EARDRLRDAGIEVDYLRVRALPLSPEIATFIQRHDRVYVVEQNRDGQLYGILRTELPT
                     HLVQRLESITHYNGVPIDAHAIIDPLLEAERVPAVVAE"
     gene            1475..>2336
                     /locus_tag="VFU90_05075"
     CDS             1475..>2336
                     /locus_tag="VFU90_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5479181.1"
                     /translation="MSVNLIGLTREVYKGLPTTLCAGCGHNSITNHLIKALYEYGVEP
                     HKLAKMSGIGCSSKTPAYFVEQAHGFNGLHGRMPSAATGAKVANRELLVMGISGDGDT
                     ASIGLGQYCHMIRRNVDLTYLIENNGCYGLTKGQFSATADVGSTQKSGKVNEYATIDV
                     CGLAIELGATFVARSFSGDGKQLVPLLQAAFSHRGTAILDIISPCVTFNDHQGSTKSY
                     AYVKDHDVVLHTADYIAGGREVAIDYEPGTVHDVQLEDGSHIVLRKLDHDYDPTDSLL
                     ALRTIHEATVR"
BASE COUNT          435 a          757 c          755 g          389 t
ORIGIN      
        1 cccttcgcgc agctcgccaa ggagcatttt gccgacaagg gcgaccttcg caagtacctg
       61 atgaacatga tctacgtcgg cgcgctggcg cgcctgctcg agattccggc cgaggcaatc
      121 gaggaagggc tcaaggcgca gttccgcacc aagccggcgg cggtcgagtc gaacatgaag
      181 gcggtgctgg tcggcttcga ttacgccagc gagcattgcg agaagcgcga cccgtaccgg
      241 ctcgagaaaa tgagcggcaa gaccgacggg atgctcttca tggacggcaa ccgcgccgcc
      301 gcgctgggct gcgtgatggg cggatgtacg gtcgccgcgt ggtatccgat cacgccgtcg
      361 tcgtcgctgt gcgagtactt cattcagtac tgcgagcgct accgcgtcga caaggagagc
      421 ggcgagcaca agttcgcaat tcttcaggcc gaagacgaac tcgcggctgc cggaatcgtg
      481 ttcggcgcgg gctgggccgg agcgcgcgcc atgacctcgg cctcgggacc ggggatctcg
      541 ttgatggccg aatacgccgg ttacggctat tttgccgaag tgccgggcgt gattttcgac
      601 gttcagcgtg ccggtccgtc gaccggcttg ccgacccgca cgatgcaggg cgacgtcgcc
      661 ttcgcctata cgctttcgca cggcgatacc aaacacgtcg tgctgctgcc ggcgaccgtc
      721 cgcgaagcgt acgagttcgc gatggaagcg ttcgacttgg ccgaccgctt ccagacgccg
      781 gtgttcgtac tgtcggatct cgatctcgga atgaactcgt ggctttcgcc gccgctgcct
      841 tacccggaga agtcgttcga ccgcggcaag gtgctcaccg cggaagatct caatcgcgtt
      901 ccgtcgtggg ggcgctaccg cgacgtcgat aaagacggca ttccctaccg caccctgccg
      961 ggcaccaagc acgccaacgc cggctttttt acgcgcggca ccggccacga cgaggaggcg
     1021 cgctactcgg agctgccgga agtgtggaag cgcaacctcg atcgcctggt tcgcaagcac
     1081 gaaacggcgc gcaccgcggt gccggcgccg gtcgtcagcg gcaaggggca ccggatcgca
     1141 attctggcct acggaaccac gcatcacgcg gtggtcgaag cgcgcgaccg cctgcgcgac
     1201 gccggcatcg aagtcgacta cctgcgggtg cgcgcgctgc cgctttcacc ggagatcgcg
     1261 accttcatcc agcgccacga ccgggtgtac gtggtcgagc aaaaccgcga cggtcaactg
     1321 tacggaatct tgcgcaccga gctgccgacg cacctcgtgc agcggctgga atcgattacc
     1381 cattacaacg gcgtgccgat cgacgcgcac gccatcatcg acccgctgct cgaggccgaa
     1441 cgcgtgccgg cggtcgtcgc ggaataggag actcatgagc gttaacttga tcggactcac
     1501 gcgcgaggtg tacaaggggc tgccgacgac cctgtgcgcc ggctgcggcc acaactcgat
     1561 cacgaaccat ctgatcaaag ccttgtacga atacggcgtc gagccgcaca agctcgccaa
     1621 gatgagcggc atcggctgct cttcgaaaac gcccgcctac ttcgtggagc aggcccacgg
     1681 cttcaacggg ctgcacggcc ggatgccgtc ggcggccacc ggcgcgaagg tcgccaaccg
     1741 cgagctgctg gtgatgggca tcagcggcga cggcgatacg gcgagcattg ggctcgggca
     1801 gtactgccat atgatccggc gcaacgtcga tctcacgtat ctgatcgaga acaacggctg
     1861 ctacggactg accaagggcc agttctcggc caccgccgac gtcggttcca cccagaagtc
     1921 cggcaaggtg aacgagtacg ccaccatcga cgtctgcggg ctggcgatcg agctgggcgc
     1981 gaccttcgtc gcgcgctcgt tttcgggcga cggcaagcag ctcgtgccgc tgctgcaggc
     2041 ggctttctcg catcgcggaa cggcgatctt ggacatcatc agcccgtgcg tgaccttcaa
     2101 cgatcatcaa ggctcgacca agagctatgc gtacgtgaag gatcacgacg tcgtgctgca
     2161 tacggccgac tatatcgcgg ggggccgcga ggtcgccatc gactacgagc cggggaccgt
     2221 gcacgacgtt cagctggaag acggctcgca catcgtgctg cgcaaactcg atcacgacta
     2281 cgatccgacc gattcgctgc tcgcgctgcg gacgattcac gaagccaccg tgcgcg
//
LOCUS       DASUCJ010000426         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1257853, whole genome shotgun sequence.
ACCESSION   DASUCJ010000426 DASUCJ010000000
VERSION     DASUCJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257853"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFU90_05080"
     CDS             <1..1366
                     /locus_tag="VFU90_05080"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU5479182.1"
                     /translation="AFGLDEKEFLGDGVITGRATVGGRQVFLFSQDFTVLGGSLGEAY
                     AEKICKTMDLALRTGAPVIGINDSGGARIQEGVVSLGGYAEIFWRNVACSGVIPQISL
                     VAGPCAGGAVYSPAITDFIVMTEKISQMFITGPEIIKTVTGEDVTFEELGGAMTHATR
                     SGVAHLVASDEDDLVDLAKLLLSYVPQNNLEDPPRFACDDDPHRSLDALDELVPDSPS
                     KPYDMRAVVTAVLDYEEFFEIQPHYAANILCGFGRVDGRSVGVVANQPSVLAGVLDIA
                     SSIKAARFVRFCDAFNIPLLTFVDVPGFLPGIDQEYGGIILHGAKLLYAFAEATVPKI
                     TVITRKAYGGAYDVMASKHIRADLNIAWPTAEIAVMGAEGAVKTIFRRELAAASDPEA
                     KMRELIDEYTERFASPYIAAQRGYVDDVVEARRTRSVVATALDMLANKRVERPKRKHG
                     NIPL"
     gene            1458..>2090
                     /locus_tag="VFU90_05085"
     CDS             1458..>2090
                     /locus_tag="VFU90_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479183.1"
                     /translation="MKLVEGKRAVVTGVANRWSIATGIARKLHEHGARLALTYQGERV
                     RDEVEKLAAELGSALVVECDVSSDASLAAMRDRLATEFGGIDALVHSIAFANKEDLSG
                     KVFETSRAGFSLALDVSSYSLIALVSALRDSLNDGASVMALTYLGATQIVPNYNLMGI
                     SKAALEAIVRYLAFDLGERGIRVNAISAGPIKTLAASGIKDFGKLLTEFAA"
BASE COUNT          375 a          707 c          647 g          361 t
ORIGIN      
        1 cgcgttcggt ctcgatgaaa aagagtttct cggcgacggc gtgatcaccg ggcgagcgac
       61 cgtcggcggc cgtcaggtct tcctcttctc gcaggatttc accgtcctcg gcggatcgct
      121 cggcgaagcc tacgccgaaa agatttgcaa gacgatggac ttggcgctgc gcaccggcgc
      181 gccggtgatc gggatcaacg attccggcgg cgcgcgcatt caagaaggcg tcgtgtcgct
      241 gggcggctac gccgaaatat tctggcgcaa cgtcgcgtgc agcggcgtga ttccgcagat
      301 cagcttagtt gccgggcctt gcgccggcgg cgcggtttac tcgccggcga ttaccgactt
      361 catcgtgatg accgaaaaga tctcgcagat gttcatcacc ggcccggaaa tcatcaagac
      421 ggtaaccggc gaggacgtta ccttcgaaga gctgggcggc gcgatgacgc acgccacccg
      481 cagcggggtc gcgcatctgg tcgcttccga cgaagacgat ctggtcgatc tcgccaagct
      541 gctgctctcg tacgttccgc aaaacaatct cgaggatccg ccgcgcttcg cctgcgacga
      601 cgatccgcat cgttcgctcg acgcactcga cgagctcgtc cccgactcgc ccagcaaacc
      661 gtacgacatg cgcgcggtcg tgaccgccgt tctcgactac gaagagttct tcgagattca
      721 gccgcattac gccgccaaca ttctgtgcgg cttcggccgc gtcgacggcc gcagcgtcgg
      781 cgtggtcgcc aatcagccca gcgtgctggc gggcgtgctc gacatcgcct cctcgatcaa
      841 ggcggcccgc ttcgtgcgct tctgcgacgc cttcaacatc ccgctgctca ccttcgtcga
      901 cgttcccgga tttttgccgg gcatcgatca agagtacggc ggcattattc tgcacggcgc
      961 caagctgctg tacgcgttcg ccgaagccac cgtcccgaag atcacggtca tcacccgcaa
     1021 agcctacggc ggcgcctacg acgtcatggc cagcaagcac atccgcgccg atctcaatat
     1081 cgcgtggccg accgcggaaa tcgcggtgat gggcgccgaa ggcgccgtga aaacgatctt
     1141 ccggcgcgag ctcgccgccg cgagcgatcc ggaggcgaag atgcgcgagc tgatcgacga
     1201 gtataccgaa cgcttcgcga gcccctacat cgccgcacag cgcggctacg ttgacgacgt
     1261 cgtcgaagcc cgccgcaccc gcagcgtcgt cgcgaccgcg ctcgacatgc tcgcgaacaa
     1321 acgggtcgag cgccccaaac gcaaacacgg caacatcccg ctgtaagcga ctgcaacgac
     1381 gcgacgccgc cctcgtcccc gccgtcgccg gcgcgcttcg gcaggacgcg cccgctcgcg
     1441 ctggtatgta gtccggcgtg aaactcgtag aaggaaaacg cgccgtcgtt accggcgtcg
     1501 caaatcgctg gtcgatcgcg accgggatcg cgcgcaagct ccacgagcac ggcgcgcggc
     1561 tcgcgctgac ctatcagggc gaacgcgttc gcgacgaggt cgagaagctc gccgccgagc
     1621 tcggcagcgc gctggtcgtc gaatgcgacg tctccagcga cgcgtcgctg gcggcgatgc
     1681 gcgaccggct cgcaaccgag tttggcggca tcgacgcgct ggtgcactcg atcgcgttcg
     1741 ccaacaaaga ggatctctcc ggaaaagtgt tcgaaacgtc gcgcgccggt ttctcgctcg
     1801 cgctcgacgt ctcgtcgtac tcgctaatcg ccctggtgag cgcgctgcgc gactcgctta
     1861 acgacggcgc ttcggtgatg gcgctgacct atctgggagc gacccaaatc gttccgaact
     1921 acaatttaat ggggatatcc aaggcggcgc tcgaggcgat cgtgcggtat ctcgccttcg
     1981 atctcggcga acgcggcatt cgcgtcaacg cgatctcggc cgggccgatc aagacgctgg
     2041 ccgcctcggg aatcaaggat ttcggcaagc tgctgaccga gttcgccgcc
//
LOCUS       DASUCJ010000427         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_340856, whole genome shotgun sequence.
ACCESSION   DASUCJ010000427 DASUCJ010000000
VERSION     DASUCJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340856"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2162)
                     /locus_tag="VFU90_05090"
     CDS             complement(117..2162)
                     /locus_tag="VFU90_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5479184.1"
                     /translation="MRELAAATIGALAIASVVWSPIAYAKPPGSGIEPAYVPGYVLVS
                     PRPGLPPSAFKGLLAAHGGNAVGRVGKLNVYVVDLAQTGGEKAIAQALSKNPNIKFAE
                     PDALVASSFVPNDAYYTSEWHLQTIGAPSAWNFATGNGVTVAILDSGIDPTHPDLATQ
                     LVPGWDFYDNSSNTADVYGHGTMVAGVVAAIGNNGIGVSGVAFNARLMPIRVTDTNGY
                     ASISALASGLSYAADHGARVANMSFAVQSYSTIISAAQYFMSKGGVVVNSAGNYGTLD
                     STAPSGALVSVSATGSTDAIASWSSYGPYVDVSAPGVSIWTTTMGGSYGAVSGTSFSS
                     PITAGVVALMMSANPNLAPTQIVSMLESTAVDLGTAGYDYYYGYGRVNAASAVLAAAE
                     GAASDTQVPSASITSPTGGTVSGIVAVNVSASDNVGVTHVDLYVNGGLLASDTVAPYG
                     FSWDSSALAGTSATLVAKAYDAAGNVGASKQVTVSVVSAAPPDTTPPTVSIASPTGGS
                     VSGVVPVSVNATDNVGVTRVDLLVNGAVVASDTTAPYTFTWDSSARIGTNVTLVATAY
                     DAAGNSASSQAVTVSVTSAAAPSNPDATPPSVTISNPPPGSTVSGIVSVSASANDNVA
                     VASISLAIDGSTVATTNSASISYRWNTKKVSKGTHTISATARDTSGNQSTTTIQVRR"
     regulatory      complement(2377..2465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          462 a          829 c          836 g          502 t
ORIGIN      
        1 cggcaatctg ccggcggttt cgtttggata atgcgcgagg accgcgaagt tggcagtcgt
       61 cggccgcgat cacctcgacc gcaaatcact ccggaagatc ggttgcgcga ccgacctcac
      121 cgccgcacct gaatcgtcgt cgtcgactga ttgcccgacg tatcacgcgc ggtcgcgctg
      181 atcgtgtgcg ttcccttgct cactttcttc gtgttccagc gatagctgat cgacgcgctg
      241 ttggtcgtcg cgaccgtact cccgtcgatc gcaagcgaga tgctcgctac ggcgacgttg
      301 tcgttggccg acgcgctgac gctcactatg ccggaaacgg tgctcccggg cggcggattg
      361 ctgattgtta cgcttggcgg cgtggcatcg ggattagacg gtgcggcggc cgacgtaacg
      421 gataccgtga cggcctgcga cgaagctgaa ttgccggcgg catcgtaagc ggtcgccacc
      481 agcgtgacgt tcgttccgat gcgtgccgac gaatcccacg tgaacgtata cggtgccgtg
      541 gtgtcgctcg cgacgaccgc gccattgacg agcaggtcca cgcgcgtgac gccgacatta
      601 tcggtcgcat tcacactcac gggcacgact ccgctgacgc tcccgcccgt cggagacgcg
      661 atgctgacgg taggtggcgt ggtgtcgggc ggcgcagcac tcacgaccga caccgtcact
      721 tgcttcgatg ctccgacgtt gccggcggca tcgtaggcct tggcgacgag cgttgcgctc
      781 gttccggcaa gcgcgctcga atcccagcta aagccgtagg gcgctactgt gtcggacgca
      841 aggagaccgc cgtttacata gagatcgacg tgcgtgacgc cgacgttgtc gctagcgctg
      901 acgtttaccg cgacgattcc gctcacggtt cccccggttg gcgaggtgat gcttgcgctc
      961 gggacttggg tgtcggacgc agcaccttct gccgcggcaa gcaccgcgga cgccgcgttc
     1021 acgcggccgt aaccgtagta ataatcgtag cccgccgttc cgaggtcgac tgccgtcgat
     1081 tcgagcatgc tcacgatctg ggtcggagcg agattcggat tggccgacat catcaatgcg
     1141 acgacacccg ccgtgatcgg actcgagaac gacgttccgg agaccgcgcc gtaactgccg
     1201 cccatcgtcg tcgtccagat gctgacaccg ggagctgaaa cgtcgacata tggaccgtag
     1261 ctcgaccagc tcgcgatcgc gtcggtgctg ccggttgccg acaccgagac aagtgcgccg
     1321 ctcggcgcgg tcgaatcgag cgttccgtag ttcccggccg agttgacgac gacgccgccc
     1381 ttgctcatga aatattgggc cgccgaaatg atcgtcgagt acgactgcac cgcgaagctc
     1441 atgttggcga cccgggcgcc atgatccgcg gcgtagctaa ggccgcttgc gagcgcgctg
     1501 atcgacgcgt agccattggt atcggttacg cgaatcggca tcaggcgggc attgaaggca
     1561 acgcccgata ctccgatgcc gttgttcccg atcgcggcga caactcccgc gaccatggtg
     1621 ccgtgcccgt agacgtcggc ggtgttgctg ctgttgtcgt agaagtccca tccggggacg
     1681 agctgcgtcg cgagatccgg atgcgtcgga tcgataccgc tgtcgaggat cgcaaccgtc
     1741 acaccgttcc ccgtggcgaa attccatgca ctcggcgccc cgatcgtttg cagatgccac
     1801 tcgctcgtgt aatacgcgtc gttcggtacg aagcttgaag caaccagggc gtccggctcc
     1861 gcgaacttga tattcggatt cttggaaagt gcctgcgcaa tcgccttctc gccacccgtc
     1921 tgcgcaaggt ctacgacata gacattgagc ttgccgacac gacccacggc attcccgcca
     1981 tgggcggcga gcaatccctt gaacgccgag gggggcaagc caggacgggg tgacacgagt
     2041 acgtagccgg gaacgtaggc aggctcgatt cccgatcccg gcggcttggc gtaggcgatc
     2101 ggcgaccaga cgaccgacgc gatggcgagc gcgccgatcg tcgccgcagc caactcgcgc
     2161 acgcacgatg caaaggtgga agtggtacgg tgggtcgccc gctgccgaat ggtttggaat
     2221 acgcgccgca tggaatcgcc tccggatcgt ggttgaccac ccacgagccc acgaggcgaa
     2281 gaattggcgg gtcttcgcgt cgacgcgtgc cgctcgacga tcgtgaagcc cgcttgcaga
     2341 ccgcagcgcg agacgcgcat ggcgccgcgc gatccgtccg gcaatcccgc tgtcgtaggg
     2401 cccgagagcc cgttagaccg gtgactttgc gcccccggct ttcgccgggt ttgcccttgt
     2461 acagaccgat ctttcgatat gacgttggca ttctaacgcg tgtgcctgcg tgagtcacaa
     2521 tgatttcgcg ccgccgagtg atattttcga cgaacggttg ctgcccaagg gcgatggcaa
     2581 ctgatgtcgc aattcttgca acgtaaccgc ggattgcttc ccatgtagc
//
LOCUS       DASUCJ010000428         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_677118, whole genome shotgun sequence.
ACCESSION   DASUCJ010000428 DASUCJ010000000
VERSION     DASUCJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677118"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2038
                     /locus_tag="VFU90_05095"
     CDS             <1..>2038
                     /locus_tag="VFU90_05095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479185.1"
                     /translation="YGLGGVTLDFEEVPPSLTDAIVKFEKRLKAKLGPQGRLVTQAVS
                     FNDNDVQLAKYGAVADKLFLMLYDEHYGGGDPGPVASQEWFVRTARHMMRFIPPTKAI
                     LALGAYGYDWNDSEDSASAMTFQDVMSAVRDAAVDGQPAAIHFDTAALNPYATWRDPD
                     STDHVDWFLDGVTAYNEIIASDALGAGGVAVWRLGSEDPAIWAVIGKRGIDAPAESLS
                     VIPSGYDPEFQPKAGTGELLELRARPTDGAREIKVDPVTRLVVDEDVTRYATPWIIRR
                     FGSGSPHKIALTFDDGPDGRYTSAILDTLKSRHAPATFFVIGQNAEAHIPLLRREYAE
                     GHEIGNHTFTHPNLAFASDRVTKLQLDATERLLEAALGRRTAFFRPPYFGDAEPTTPD
                     ELEPIAVATDRGYISVGLRIDAEDWQSPGVTTIIDTVLHQLPKGGNVVLLHDGGGNRT
                     QTVAALGTLIDSIRARGDTLVPLSELVGIPRDVAMPPLPPEGRFGRFGELLAFGGVGV
                     AEWVLYWLFTIAVVLGFARLLFITTLAIIQRIKRHQNPDAPVTYAPTVSVIVPAYNEE
                     KVIVNTIRSLVTQEYPGELEVVMVDDGSPDRTMEVAQAAFGHHPRVTLLRKPNGGKAS
                     ALNFGLQRARGEIVICLDADTIFAPNTVAELVEPLHDPSVGAVAGNAKVGNRVNL"
BASE COUNT          343 a          684 c          701 g          310 t
ORIGIN      
        1 ctacgggtta ggcggcgtga cgctggactt cgaggaagtg ccgccgagtc tcacggatgc
       61 gatcgtgaag ttcgagaagc ggctcaaggc gaagctcggc ccgcagggcc gcttggtgac
      121 gcaggcggtt tcgttcaacg acaatgacgt gcagctggcc aagtacggcg cggtggccga
      181 caagctgttc ctgatgctgt acgatgagca ctacggcggc ggagatccgg ggcccgttgc
      241 gagccaggag tggttcgtcc gtacggcgcg ccacatgatg cgcttcattc cgcctaccaa
      301 ggcgatcctc gcgttgggcg cgtacggcta cgactggaac gactccgagg actcggcgtc
      361 ggccatgacg ttccaggatg tcatgagtgc ggtgcgcgac gccgcggtgg acgggcagcc
      421 ggccgcgatc catttcgata cggcggcgct caatccctat gccacgtggc gcgacccgga
      481 ttcgaccgac cacgtggact ggttcctcga cggcgtcacg gcgtacaacg agatcatcgc
      541 cagcgacgcg ttaggcgcag gcggcgtcgc ggtgtggcgt ctcgggtccg aggaccccgc
      601 gatctgggcg gtgatcggca agcgcggcat cgatgcgccg gcggagagcc tgagcgtgat
      661 cccgtcgggc tacgatcccg agttccagcc caaagccggc acgggcgagc tgctcgagct
      721 gcgcgcacgc ccaacggacg gcgcccgcga gatcaaggtc gatcccgtga cgcggctcgt
      781 cgtggacgag gacgtcacgc gatacgcgac gccgtggatc atccggcgct tcggcagcgg
      841 atcgccgcac aagatcgcgc tgacgttcga cgacgggccc gacggccgct acacgagcgc
      901 gattctcgac acgctcaagt cgcggcacgc gccggcgacg tttttcgtga tcgggcagaa
      961 cgcggaagcg cacatcccgc tgctccgccg cgaatacgcc gagggccacg agatcggcaa
     1021 ccacacgttc acgcatccga atctcgcgtt cgcgagcgac cgggtgacca agctccagct
     1081 ggacgccacc gagcggctgc tcgaggcagc gttaggcagg cgcacggcgt tcttccggcc
     1141 gccgtatttc ggcgacgccg agccgacgac gccggacgag ctcgagccga ttgccgtggc
     1201 caccgaccgc ggctacatca gcgtcggcct ccgcatcgac gccgaggact ggcagtcgcc
     1261 gggcgtcacg acgatcatcg atacggtgct gcaccagctg cccaagggcg ggaacgtcgt
     1321 gctgctccac gacgggggcg gcaaccgcac ccagacggtc gctgcgttag gcacgctcat
     1381 cgattcgatc cgcgcccggg gcgacacgct ggtgccgctc tcggagctgg tcggcattcc
     1441 gcgcgacgtc gccatgccgc cgctgccgcc cgagggccgg ttcggccgat tcggcgagct
     1501 gctcgccttc ggcggcgtcg gcgtcgccga gtgggtgctg tactggctct tcacgatcgc
     1561 cgtcgtcctc ggcttcgcgc gactgctgtt catcacgacg ctcgcgatca tccagcgcat
     1621 caaacgccac cagaatcccg acgcgccggt gacgtacgcg ccgacggtga gtgtgatcgt
     1681 gccggcgtac aacgaagaga aagtgatcgt caacacgatt cgcagcctcg tgacgcagga
     1741 atatcccggc gagctcgagg tggtgatggt ggacgacggc tcgcccgacc gcacgatgga
     1801 ggtggcgcag gcggcgttcg gccaccatcc gcgcgtgacg ctgctccgca agcctaacgg
     1861 aggaaaggcc agcgcgctca acttcggcct ccagcgcgcg cgcggcgaga tcgtgatctg
     1921 tctcgacgcg gacaccatct tcgcgcccaa tacggtcgcc gagctcgtcg agccgctgca
     1981 cgatccgagc gtcggcgcgg tggccggcaa cgcgaaggtc ggaaaccgcg tgaacctc
//
LOCUS       DASUCJ010000429         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_35200, whole genome shotgun sequence.
ACCESSION   DASUCJ010000429 DASUCJ010000000
VERSION     DASUCJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35200"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFU90_05100"
     CDS             <1..209
                     /locus_tag="VFU90_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479186.1"
                     /translation="SSNQDRDADDGAGEVGKALIVGLLGGLLSVAGYMVYRRLPDEQK
                     EKLHSQVRNAVQQRVTELRQNFNI"
     gene            275..820
                     /locus_tag="VFU90_05105"
     CDS             275..820
                     /locus_tag="VFU90_05105"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5479187.1"
                     /translation="MKIALLAFLAMPAFFTGRAAAGVPAAGGAVALYARALQHFNPRL
                     DGETARQLAAATIEQADREGLDARLLVALIAVESRWNPAARSPAGARGLGQLMPQTAA
                     SLGVDAGDPIDNIAGAARHLHALLNRFRDAGDPARFALALAAYNAGTAAVSRYGGIPP
                     FLETRVYVRRVIRLWYRLAGR"
     gene            complement(786..1070)
                     /locus_tag="VFU90_05110"
     CDS             complement(786..1070)
                     /locus_tag="VFU90_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5479188.1"
                     /translation="MFSVPDILVVSVLALLLFGPDRLPKMMRQAGRFMREVQNTSHSF
                     VAEMERAADLPEPPAPAHVPPRESAAHEATSPGLPEPPEVTGRPTDTTSG"
     gene            1153..1623
                     /locus_tag="VFU90_05115"
     CDS             1153..1623
                     /locus_tag="VFU90_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479189.1"
                     /translation="MKIIYTRGNRTETLASLATLRKILNRFLGHRVFYEVSSRSKRGH
                     EFFSTKNVFVVGLVEVTNFEHLKILIFDAHSQAHSIEILNPQTMRIYDEMPGRGFAVS
                     FLSENEGIETRCYIRDEGEDEARINARTALEKITLPQLFEYLEEITTVDAGVRS"
     gene            complement(1592..>1809)
                     /locus_tag="VFU90_05120"
     CDS             complement(1592..>1809)
                     /locus_tag="VFU90_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479190.1"
                     /translation="NRGNLLLDEGRVDDAIASYRAAILADPDYAIAHLNLGVAYKRAG
                     RIEEGVRELRLSQRLERANFLRRRRPS"
BASE COUNT          318 a          607 c          582 g          302 t
ORIGIN      
        1 ctagctccaa tcaggatcgc gatgccgacg acggcgccgg cgaagtgggg aaagcgctga
       61 tcgtcggctt gctcggcgga ctcctttcgg tcgccggtta catggtctat cgccgtttgc
      121 cggacgagca aaaggaaaag ctgcattcgc aagttcgcaa cgcagtccag cagcgcgtaa
      181 ccgaactgcg ccaaaacttc aacatctaat cgcgcgcgac cgatccgcac ccccgttggc
      241 ttgacggcct cgccaaacgg gcacttgcgg gcgggtgaag atcgcgctgc tcgcgtttct
      301 cgcaatgccg gcattcttta ccggacgcgc cgcggccggc gtacccgccg ccggcggagc
      361 ggtcgcgctc tacgcgcgag cgctgcagca cttcaatccg cggctcgacg gcgaaacggc
      421 tcggcagttg gccgcggcga cgatcgaaca agccgaccgc gaagggctcg acgcgcgctt
      481 gctggtggct ttgatcgcgg tcgaatcgcg ctggaatccg gcggcccgct cgcctgccgg
      541 cgcgcgcggc ctcggccagc tgatgccgca gacggcggcg agtctcggcg tcgacgccgg
      601 cgatccgatc gacaacatcg ccggtgcggc acgccacctg cacgcgcttt tgaaccggtt
      661 ccgcgacgcc ggcgatccgg ctcgtttcgc gctcgcactc gcagcgtaca acgcgggcac
      721 cgcagcggtg agtcgctacg gcggcatccc gccgtttctc gaaacgcgcg tctacgtgcg
      781 gcgcgtcatc cgcttgtggt atcggttggc cggccggtga cctccggcgg ttccggcagg
      841 ccgggcgacg tcgcttcgtg cgctgcggat tcgcgcggcg gcacgtgcgc cggtgccggc
      901 ggctcgggca ggtcggccgc acgctccatt tccgcaacga acgaatgcga cgtgttctgc
      961 acctcgcgca tgaaccggcc ggcttgccgc atcatcttcg ggaggcggtc cggcccgaac
     1021 agcagcagcg cgagcacgga gacgaccagg atgtccggaa cggagaacac gcgcctagtt
     1081 gcggctccgc cgggcgtttg gcctgcttgg cagggctcgc ctgcagggcc gtgcaagcac
     1141 cggagtccgt ccgtgaagat catctacacc cgcggcaacc gcaccgaaac gctggcctcg
     1201 ctggcaaccc tgcggaaaat cctcaaccgc ttcctcggcc atcgcgtctt ttacgaagtc
     1261 tcgagccgga gcaaacgcgg tcacgagttc ttttcgacca agaacgtctt cgtggtcgga
     1321 ctcgtcgagg tgacgaactt cgaacacctc aaaatcctaa ttttcgacgc ccacagtcag
     1381 gcgcattcga tcgagatcct caacccgcag acgatgcgaa tctacgacga aatgccggga
     1441 cgcggcttcg ccgtttcctt tctgagcgaa aacgaaggca tcgagacgcg gtgttacatt
     1501 cgcgacgaag gtgaggacga agctcgcatc aacgcacgga ccgcgctcga gaagatcacg
     1561 ctgcctcagc tgttcgaata cctcgaagag atcacgacgg tcgacgccgg cgtaagaagt
     1621 tagcgcgctc gagccgctgc gacaggcgca gctcccgcac gccctcttcg atgcggccgg
     1681 cgcgcttgta cgcaacgccg aggttgagat gcgctatcgc gtagtccgga tcggccagga
     1741 tggcggcgcg atagctggcg atggcgtcgt cgacgcggcc ctcgtcgagc agcaaatttc
     1801 cccgattgg
//
LOCUS       DASUCJ010000430         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_891157, whole genome shotgun sequence.
ACCESSION   DASUCJ010000430 DASUCJ010000000
VERSION     DASUCJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891157"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1199
                     /locus_tag="VFU90_05125"
     CDS             168..1199
                     /locus_tag="VFU90_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479191.1"
                     /translation="MTAAAVPIEQAKDAGLRYVSDRDPGIERLKARGGFRYVMPDGTP
                     VRDRSTLDRIARLAIPPAYTGVWICRQADGHLQATGRDARGRKQYRYHERWRKIRDAT
                     KYDRMLAFSAALPKIRRAVDRDLAKPKMPREKVIAAVVALLETTLIRIGNEEYVRANG
                     SFGLTTLRNRHARVRGAKLRFEFDGKSGIRHYVDLHDRRLARVVARCQELPGQHLFSY
                     VDDEGNAVPIDSSDVNGYIREISGDDFSAKDFRTWLATVYCARWLLEHPEESAGGRQR
                     AIVDATRAVAKALRNTPAVCRNCYIHPQLFESYRATGRLQHPTRARSGRGLSADERLT
                     IGILRSRTA"
     gene            1254..>1911
                     /locus_tag="VFU90_05130"
     CDS             1254..>1911
                     /locus_tag="VFU90_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5479192.1"
                     /translation="MPILESPAASSVPPRRITDNRVLSRLEPPILALLEASAEVRQVP
                     TNFPICEPGEAVSFFCFPLTAVASTVTCMSNGDAIEAVTIGNEGVVGISAILGGTQPT
                     LRSFIQVPGEACLVSAGTFLRIVREHPPVFEFLLKYANVLINVISQSAACNRLHNVVQ
                     RCARWLLTTADRARSEEFQLIQELLAEMVGARRPTVSDAAATLQHAGLIRYKRGEVRI
                     V"
BASE COUNT          329 a          626 c          625 g          331 t
ORIGIN      
        1 gcccactacg tgcgcgacgt cgagtcgcaa aatttcggac ccggcggcat cacgttatcg
       61 ctcgtcggcg ctgcggcgga aggtctcgtg gcgtgggtgg ctggtttggg gctcttggtt
      121 ttgctgtgcg tccggctggg agcgatcccg cacgaagaag atcgcgcgtg actgcggccg
      181 cggtgccgat cgagcaggcg aaggacgcgg gtcttcgcta cgtgagcgac cgcgatcccg
      241 gtatcgaacg tttgaaagcc cgcggcggct ttcgctacgt catgcccgat ggaacgccgg
      301 tgcgggatcg ctcgacgctc gatcgcatcg cgcgtctcgc cattccgccc gcctacaccg
      361 gcgtctggat ttgccgtcag gccgacgggc atcttcaggc aaccggccgc gacgcccgcg
      421 gtcgcaagca ataccgctac cacgagcgct ggcgcaaaat acgcgacgcg acgaagtacg
      481 atcgaatgct cgctttttcg gcggccctgc caaagattcg ccgtgccgtc gaccgcgatt
      541 tggcaaaacc gaagatgccg cgcgaaaagg tcatcgccgc ggtcgtcgca ctgctcgaga
      601 ccaccctgat tcgcatcgga aacgaggaat acgtgcgcgc gaacgggtcc ttcggtctga
      661 cgacgctccg aaatcggcac gcgcgggttc gcggagccaa attgcgcttc gagttcgacg
      721 gcaagagcgg catccgtcac tacgtcgacc ttcacgatcg gcgcctcgct cgagtcgtcg
      781 cgcgatgcca agagctcccg gggcaacacc tgttcagcta cgtcgacgac gaagggaacg
      841 cggtgccgat cgactcgtcc gacgtcaacg gctatatccg ggagatctcg ggcgacgact
      901 tttcggcgaa agacttccgg acgtggctgg cgacggtgta ctgcgcgcga tggctcctcg
      961 agcatccgga ggaatcggcc ggcggccgcc agcgggcgat cgtcgacgcg acgcgtgcgg
     1021 tcgccaaagc gctgcgaaac acaccggcgg tgtgccggaa ctgttacatc cacccgcagc
     1081 tgttcgaatc gtatcgcgcg acgggacggc tgcagcaccc aacccgagcc cgttctggtc
     1141 gcgggctgtc ggccgacgaa cggctgacca tcggcatatt gcggtcgcga accgcctagg
     1201 aatccaccgc ggcccggatt agcgaccgcg gcgaaccggg tatacggact gtcatgccga
     1261 ttttggaatc gccggccgct tcgagcgtgc cgccccgccg gattaccgac aatcgcgtgc
     1321 tctcgcggct cgagccgccg atcctggcac tgctggaagc ctccgccgaa gtccgccagg
     1381 tcccgacgaa ttttccgatt tgcgagccgg gcgaagcagt gtcgttcttc tgcttcccgc
     1441 tgacggccgt ggcttcgacg gtgacctgca tgagcaacgg cgacgccatc gaggcggtga
     1501 ccatcggaaa cgaaggcgtg gtcggaatat cggcaatctt gggcggaacg cagccgacgc
     1561 tgcggtcgtt catccaggtt cccggcgagg cttgcctcgt ctcggctggg acgttcttac
     1621 gaatcgttcg cgagcacccg ccggtgttcg agtttctcct gaagtacgcg aacgtgctga
     1681 tcaacgtcat ctcgcagtcg gcggcgtgca accggctcca caacgtcgtc caacgttgcg
     1741 cgcgctggct gctgacgacc gcagaccgcg cgcgcagcga ggaattccag ctgattcaag
     1801 agctgctcgc ggagatggtc ggggcgcgcc ggccgacggt ttccgacgca gccgccaccc
     1861 tgcagcacgc cggcctcatc cgctataagc gcggcgaggt gcgcatcgtc g
//
LOCUS       DASUCJ010000431         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_264473, whole genome shotgun sequence.
ACCESSION   DASUCJ010000431 DASUCJ010000000
VERSION     DASUCJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264473"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFU90_05135"
     CDS             complement(<1..373)
                     /locus_tag="VFU90_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479193.1"
                     /translation="MKFKSWPSVAALAALPFAIHPAARASDAGCAITIFPRMGTFEPS
                     GRLYGPGLVLAGGGAEEAPASMFPWMRRRLAGPRSGRFGNIVFLRASDTTEYDKLFYD
                     GGAFRSVQTVLIPPCAPRAQVD"
     gene            complement(389..>1333)
                     /locus_tag="VFU90_05140"
     CDS             complement(389..>1333)
                     /locus_tag="VFU90_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5479194.1"
                     /translation="DVVDTQIDPVQSVARDGTLAFVGTAAGRPNELYALAPGASAPQR
                     LTDFNAAVAALQLGRVTTVRWDSCDGFHEAGVLTYPPGYVAGKRYPPVLRIHGGPNET
                     SEAFFEPFYQLAAQRGYLVFAPNYRGSTNLGSAFERAIFNDASDGPGRDIVDGIAAVE
                     RLGIVDRSRLAVSGWSYGGQLTSWLIGRYHIWKCAVTGAAVNDLVLDYTIADDLVAAR
                     EAFDAPPFSEKLSAWQRQSPITYVRDVRTPLLMFGNVYDVRVPIAEQYEMYRALRDNR
                     VPVRFFVYPSGGHLPQGPNRLASVYRRWLAWFDRYLKA"
BASE COUNT          239 a          416 c          477 g          201 t
ORIGIN      
        1 gatcgacttg cgcgcgcggg gcgcacggcg gaatcaacac cgtttgcacc gatcgaaacg
       61 cgccgccgtc gtaaaacagc ttatcgtact cggtggtatc ggaggcgcgc aagaagacga
      121 tgttgccgaa gcgcccggag cgcgggccgg ccagccggcg ccgcatccac ggaaacatcg
      181 aggcgggtgc ttcctcggcg ccgccgccgg cgagcaccag accgggcccg tagagccgtc
      241 cgctgggttc gaaggttccc atgcgcggaa agatcgtgat cgcgcaaccg gcgtcggaag
      301 cgcgcgcggc aggatggatt gcgaaaggca gcgctgcgag cgcggcgacc gacggccaag
      361 atttgaattt catgctttca gataccggtc atgctttcag atagcggtcg aaccacgcga
      421 gccagcggcg atagacggag gccaaacggt tggggccttg cggcaaatgg ccgccgctgg
      481 ggtagacgaa gaatcgaacg ggaacgcgat tgtcgcgcag cgcgcggtac atttcgtact
      541 gttcggcgat cggaacgcgc acgtcgtaga cgttgccgaa catcagcagc ggcgtgcgca
      601 cgtctcggac gtaggtaatc ggggactgcc gctgccaggc ggagagcttc tcgctgaacg
      661 gcggagcgtc gaaggcctcg cgcgcggcca cgaggtcgtc ggcgatcgtg tagtcgagca
      721 cgaggtcgtt gacggcggcg ccggtcacgg cgcacttcca gatatggtag cggccgatta
      781 gccacgaggt cagctgcccg ccgtacgacc agccggaaac cgccagtcgc gatcggtcga
      841 cgattccgag ccgttccacg gccgcgattc cgtccacgat atcgcgtccg ggcccgtcgc
      901 tggcatcgtt gaagatcgcg cgctcgaacg cgctgccgag attggtgctg ccgcggtagt
      961 tgggtgcgaa gaccaggtag ccgcgctgcg ccgcgagctg atagaacggc tcgaaaaacg
     1021 cctccgaggt ttcgttcggg ccgccgtgga tgcgcaatac gggcggatag cgtttccccg
     1081 cgacgtagcc gggcggatac gtcagcacgc cggcttcgtg aaatccgtcg cacgaatccc
     1141 agcgaacggt ggtgacgcgg ccgagctgca gcgcggcgac ggcggcgttg aaatcggtca
     1201 gccgctgcgg cgcggaggcg cccggcgcga gcgcgtagag ctcgttgggc cgtccggcgg
     1261 cggtcccgac gaaggccagc gtgccgtcgc gcgcgacgct ctgcaccgga tcgatctgcg
     1321 tatcgaccac gtc
//
LOCUS       DASUCJ010000432         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_585461, whole genome shotgun sequence.
ACCESSION   DASUCJ010000432 DASUCJ010000000
VERSION     DASUCJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585461"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFU90_05145"
     CDS             <1..734
                     /locus_tag="VFU90_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5479195.1"
                     /translation="EVAGLAGLERLTFISPHPKDFTEKIVASLGHLTRLNPRVHLPLQ
                     SASDRVLRRMNRKYTIAEFHDKAELLRRHIPDCAITTDIIVGFPGETEDDFERTLDYV
                     RTGAFANAYTFMYSIRRGTPAARWEQVPREVATARFARLVEAQNAVTRAHHDRKLAKT
                     VRALVAGDSKKDASKLAVKALDNVTIVVPKPDDYDVDTYAREPWVDVEIESAHLWGCT
                     GSIARRSARYDLPGSAVKRTLVSLL"
     gene            861..>1252
                     /locus_tag="VFU90_05150"
     CDS             861..>1252
                     /locus_tag="VFU90_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU5479196.1"
                     /translation="MQSMPPSGAAPRGSTARSAGFTTEFVGFWESWSDTNKRDAFYRL
                     GSVPSQVTTADVAFSIADSNKIANPQNTYPLRSGARRIHARGGKVLLSFGGATSNFEI
                     TDPNAFVRNLKAYLKAHPGVYDGFDFDD"
BASE COUNT          227 a          402 c          401 g          222 t
ORIGIN      
        1 gggaggtcgc gggacttgcg gggctggaac gcctgacctt catctcgccg catcccaagg
       61 acttcaccga aaagatcgtc gcgtcgctcg ggcacctgac gcggctcaat ccgcgggttc
      121 atttgccgct gcaatccgcc agcgatcggg ttctccggcg gatgaaccgg aagtacacga
      181 tcgccgaatt tcacgataag gccgagctgc ttcggcgcca cattccggat tgcgcgatca
      241 cgaccgacat catcgtcggg tttcccggag aaaccgagga cgacttcgaa cgaacgctcg
      301 actacgtgcg caccggcgcg ttcgccaacg cgtacacctt catgtattcg attcgtcgcg
      361 ggacgccggc agcgcgctgg gaacaggttc cgcgcgaggt cgctacggcg cgtttcgcgc
      421 ggctggtcga ggcgcaaaat gccgtcaccc gcgcgcatca cgatcgaaaa ctcgcgaaga
      481 cggtacgcgc gctggtagcc ggcgattcga agaaggacgc gagcaaactc gcggtcaagg
      541 cgctcgacaa cgtcacgatc gtcgttccga agccggacga ttacgacgtg gatacgtacg
      601 cgcgcgagcc gtgggtcgac gtcgagatcg agtcggcaca cttgtgggga tgcaccggat
      661 cgatcgctcg gcggtcggcg cgctacgatt tgcccggcag cgcggtgaag cgcacgcttg
      721 taagccttct ctaatctggg cgggacgccg accacaggag cggttcccgc tcgcccgcaa
      781 gctttcgggg tatgagccgg ctacgcgctg tcctggcagc ctcgctggtc gcggccttga
      841 cggcgtgcgg cggaggtacc atgcagtcga tgccgccttc gggtgcggcg ccgcgcggaa
      901 gtacggcccg ctcggccggg tttaccaccg agttcgtcgg tttttgggag tcgtggtcgg
      961 acaccaacaa acgcgacgcc ttctatcgcc tcgggagcgt gccgtcgcag gtgacgaccg
     1021 ccgacgtcgc tttcagcatc gccgacagca ataaaatcgc gaatcctcag aatacctatc
     1081 cgcttcgctc gggcgcgcgc cgcattcacg cgcgcggcgg aaaggttctg ctctcgttcg
     1141 gcggagcgac ctcgaacttc gagattaccg acccgaacgc gttcgtgcgg aatctcaagg
     1201 cgtatctcaa agcccatccc ggcgtttacg acggcttcga cttcgacgac ga
//
LOCUS       DASUCJ010000433         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_524221, whole genome shotgun sequence.
ACCESSION   DASUCJ010000433 DASUCJ010000000
VERSION     DASUCJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524221"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFU90_05155"
     CDS             complement(<1..977)
                     /locus_tag="VFU90_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5479197.1"
                     /translation="MNKGIEIARGKTKVLYEVPGQPAQLVVAQTDQISAGDGARRHEI
                     PGKGRLAAQTTARVFRLLNLCGLPTHYLNGGEDDDDNEMVVRRCNMIPLEVVARGVAA
                     GSFVKRNPAISRGMLLVPRVIEFFLKDDARHDPLIAPESVVAENIAAPHEVAAMTELA
                     RLTFDILAHAWRRRDVMLVDLKIEFGRLASGENQGQLVIADVIDNDSWRIWPQGREDL
                     MLDKQRYRNLETVDDAALAEVKRGYERAADMVGSFPVMRPGMAAVIADATAQVERVNE
                     IVGALGQLGMPSVRHVASPATTPGYVLQLVAQLETSFARLAFVAVGSDDL"
     gene            complement(970..2412)
                     /gene="purB"
                     /locus_tag="VFU90_05160"
     CDS             complement(970..2412)
                     /gene="purB"
                     /locus_tag="VFU90_05160"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5479198.1"
                     /translation="MDYHTYASPFSWRYGRASLRALFSEQTRRRLWRAAWVALAEAQG
                     AQGLVGEDELEDLRAHAGRVDIEAALAIEREIHHDLMAEIRVFASQAKLGGGKLHLGA
                     TSMDIEDTVETYRLRLALAWIGESLHGLLAAFATQIRRYADLVCMGFTHLQPAEPTTL
                     GYRLAVYAQDLLIDDANLRFVFDNLTTKGFRGAVGTAASYEQLLDRSGRSADVEARVL
                     DYFGLQAREISTQTYPRKLDYLLLAGLAGLGASLSKFAHDVRVLGSPAFGEVGEPFGR
                     KQVGSSAMPFKRNPILSERIDSLARLLVGYSDVAWQNAATNLLERTLDDSANRRTILP
                     EALLCSDEIVSLARKTIDGLRVDDRKIAQNLRTYGPFAGTEAVMMEAVRSGGDRQVLH
                     EAIRNASMESWEALAAGQDNPLSRLLTEDRELTALVDPAEIRRLLDPGKHVGTAPERA
                     RQLAARIDRLEPFPQQPEVVLASAGSQTHE"
     gene            2559..>2890
                     /locus_tag="VFU90_05165"
     CDS             2559..>2890
                     /locus_tag="VFU90_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479199.1"
                     /translation="MRYAAAITCALLASVVSAGCTRTATSPPAGRHAWSVPGELRVAV
                     VQEPKSLNPLLATTTADVFVDRLMFEPLLSADPRGNPVPMLATEVPTTGNGGISRDGL
                     TIVYHLRE"
BASE COUNT          478 a          994 c          921 g          497 t
ORIGIN      
        1 agatcgtccg atcccaccgc tacgaaggcc agccgcgcga acgacgtttc cagctgcgcg
       61 accagctgca gaacgtagcc cggggtcgtg gcgggactcg cgacgtgacg caccgacggc
      121 attccgagct ggcccagcgc gccgacgatt tcgtttacgc gctcgacctg cgccgttgcg
      181 tcggcgatca ccgccgccat ccccgggcgc attaccggaa acgaaccgac catgtcggcg
      241 gcccgctcgt acccgcgctt cacctccgcc aacgcggcgt cgtcgaccgt ttcgaggttg
      301 cggtagcgct gtttgtcgag catcaagtct tcgcggcctt gcggccagat tcgccacgag
      361 tcgttatcga tcacgtcggc gatcacgagc tggccttggt tctcgccgct cgcgagccgg
      421 ccgaattcga ttttcagatc gaccagcatc acgtcgcgcc gtcgccacgc gtgcgccagg
      481 atgtcgaacg tcaaccgggc gagctcggtc attgccgcga cttcgtgcgg cgccgcgatg
      541 ttttcggcga cgacgctttc gggggcgatc agcgggtcgt gccgcgcgtc gtccttcagg
      601 aaaaactcga tcacgcgggg caccagaagc atcccgcgcg atatcgccgg attgcgtttg
      661 acgaacgaac ccgccgcgac gccgcgcgcc acgacttcga gcgggatcat gttgcagcgg
      721 cgcacgacca tctcgttgtc gtcgtcgtct tcgccgccgt tgagataatg cgtcgggagc
      781 ccgcacaggt tcagcagccg gaacacgcgc gcggtggtct gcgccgcgag ccggcccttg
      841 cccgggatct cgtgccggcg ggcgccgtcg cccgcggaga tttgatcggt ttgggcgacc
      901 accagctgcg ccggctggcc cggaacctcg tagagcacct tcgtcttgcc ccgggcgatc
      961 tcgatacctt tattcatgcg tttgcgatcc tgcggaagcg agcaccactt cgggctgctg
     1021 cggaaacggc tccaaacggt cgatccgggc ggccagctgc cgcgcgcgtt cgggagcggt
     1081 gccgacgtgc ttgcccggat cgagcaaacg tcgaatctcc gccggatcca ccagcgcggt
     1141 cagctcgcga tcctccgtca gaagccgcga cagcggattg tcttgaccgg cggccagcgc
     1201 ctcccacgat tccatcgaag cgttccggat cgcttcgtgc agcacttgcc ggtcgccgcc
     1261 ggagcggacc gcttccatca ttaccgcctc cgtgccggca aacggaccgt aggtccgcag
     1321 gttctgcgcg atcttgcgat cgtccacccg caatccgtcg atcgtcttgc gcgccagcga
     1381 cacgatttcg tcgctgcaaa ggagcgcttc gggaagaatc gtgcggcggt tcgcgctgtc
     1441 gtcgagcgtg cgttcgagca gattggtcgc ggcgttctgc cacgcgacgt cgctgtagcc
     1501 caccagcagc cgcgcgagcg aatcgatacg ttcgctcaag attggattgc gtttgaaggg
     1561 catcgccgag cttcctacct gtttgcggcc gaagggctcg cctacctcgc cgaatgcggg
     1621 actgccgagc acgcgcacgt cgtgagcaaa ctttgacagc gacgcgccca aaccggccaa
     1681 gcccgcgagc agcaggtagt cgagcttgcg cggataggtt tgcgtgctga tttcgcgcgc
     1741 ttgcagcccg aagtaatcga gcacacgtgc ctcgacgtcg gccgagcggc ccgaacggtc
     1801 gagcaattgt tcgtacgacg ccgccgtccc gacggcgccg cggaagccct tggtcgtgag
     1861 attgtcgaag acgaatcgca aattggcgtc gtcgatcagc aaatcttgcg cgtagacggc
     1921 cagccggtag ccgagcgtcg tcggctcggc gggctgcaga tgcgtgaatc ccatgcacac
     1981 gagatcggca tagcggcgaa tttgggtcgc gaaggcggcg agcagcccgt gcaggctttc
     2041 gccgatccac gccagcgcca gccgcagacg atacgtttcg accgtatcct cgatgtccat
     2101 cgacgtcgca ccgagatgca gcttcccgcc gccgagcttt gcctgcgatg caaaaacccg
     2161 tatttcggcc atcagatcgt ggtggatctc gcgctcgatc gccaacgccg cctcgatgtc
     2221 gacgcggccg gcgtgcgcgc gcaaatcttc gagctcgtcc tcgccgacga gaccctgggc
     2281 accctgcgcc tccgccagcg caacccacgc ggcgcgccac agccggcggc gcgtttgctc
     2341 ggagaagagc gcgcgcagcg aggcgcgtcc gtatctccac gaaaacggcg aagcgtaggt
     2401 atggtaatcc atggggtacg ctcccgttcg ccccaccccc aaccgtgtca tcctgagcgg
     2461 agctcggtga gctacgctca ccgagcgcag tcgaaggacg aagggcgaag ggcccgagcg
     2521 cacagggctt cctcgccggg ccaaggtagt tgcgacttat gaggtatgcc gccgcgatta
     2581 cgtgcgcgct gctcgcgtcg gtagtctcag ccggctgcac gcgcaccgcg acgagcccgc
     2641 cggcgggccg tcacgcctgg agcgttcccg gcgagctgcg cgtcgccgtc gttcaggagc
     2701 ccaagagtct caacccgctg ctcgcgacca cgacggccga cgtgttcgtc gatcgcctga
     2761 tgttcgagcc gctgctttcc gccgatccgc gcggcaaccc cgtaccgatg ctcgcgaccg
     2821 aagttccgac gaccggcaac ggcggcatca gccgggacgg cctaacgatc gtctaccacc
     2881 tgcgagaaga
//
LOCUS       DASUCJ010000434         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_783854, whole genome shotgun sequence.
ACCESSION   DASUCJ010000434 DASUCJ010000000
VERSION     DASUCJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783854"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFU90_05170"
     CDS             complement(<1..963)
                     /locus_tag="VFU90_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5479200.1"
                     /translation="MRIVVVGATGNVGTSVVRALSARPDIDEIIGIARRLPMTAEPKT
                     GYETADIARDDLVPHFAGADAVFHLAWLFQPTHDPVATWQTNAVGSVRVFDAVAAAGV
                     KTLLYASSVGAYSPGPDEVVDESWPTHSNPAAGYGREKAYVERALDAFEARHLDVRCV
                     RFRPAFIFKRGAASAQRRLFAGPAMPNAIARPGLLPAVPLPAGLRFQAVHTRDVADAF
                     VRALMRDVRGAFNIASEPVIDGPLLARVLGGRAIEVPRGVVRAVLASAWQLHLVPSEP
                     ALFDLFLQLPLLDCSRARTELEWMPAVSAIDAIAEALTGIAEGAG"
BASE COUNT          228 a          425 c          404 g          160 t
ORIGIN      
        1 gcccgccccc tcggcgatgc cggtcagggc ttcggcaatc gcgtcgatgg cagacaccgc
       61 gggcatccat tcgagctcgg tccgggcgcg cgagcaatca agcagcggca actgcaagaa
      121 caagtcgaac agtgcgggtt ccgacggcac gaggtgcaac tgccacgcgc tcgccagtac
      181 ggcccggacg acaccccgcg gcacctcgat cgcgcgaccg ccgagtaccc gggcgagcag
      241 aggcccgtcg atgacgggct ccgacgcgat gttgaacgca ccgcggacgt cgcgcatcaa
      301 cgcgcgcacg aacgcgtcgg cgacgtcgcg cgtgtgcacc gcctggaaac gtaatccggc
      361 cggtagcggg accgccggga gaagtcccgg gcgcgcgatc gcgttcggca tcgccggacc
      421 cgcgaagagc cgtcgctgag ccgacgcagc gccgcgcttg aagatgaacg ccggacggaa
      481 acgaacgcac cgcacgtcga ggtgacgcgc ctcgaacgcg tccagcgcgc gctcgacata
      541 cgccttctcg cgtccgtacc cggcagccgg gttgctgtgc gtcggccatg attcgtcgac
      601 gacttcatca ggtcccggcg aatacgcacc aaccgaagac gcgtacagca acgtcttcac
      661 accggcggcg gccaccgcgt cgaagacgcg cacgctcccg accgcgttcg tttgccaggt
      721 cgcgaccggg tcgtgcgtcg gttggaacag ccacgcgaga tggaacaccg cgtcggctcc
      781 ggcgaaatgc ggcacgagat cgtcacgcgc gatgtcggca gtctcgtaac cggtcttcgg
      841 ttcggcggtc atcggcaaac ggcgcgcgat cccgatgatc tcgtcaatat cgggacgcgc
      901 cgagagcgct ctgacgacgc tcgttccaac atttcctgta gctcccacaa cgacgattcg
      961 catcgtgcgc gcgttcccgc gacggcccgc cgctaacccg caccgccggt ccgatgtacg
     1021 gtgcgaacgc ggtggggcaa gggaggacgc gacatgacgg atctggcagc ggacgcaggc
     1081 gaacagctcg agaatggccg gcgaccctcg acgccgcgcg gcgacaacct tctctgggac
     1141 ttcatcgacg ccgaagcgag cggctacggc gcaatcgcag cggccaagaa ggcagcccga
     1201 cgcgtcgacg aacggtt
//
LOCUS       DASUCJ010000435         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_646599, whole genome shotgun sequence.
ACCESSION   DASUCJ010000435 DASUCJ010000000
VERSION     DASUCJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646599"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..249)
                     /locus_tag="VFU90_05175"
     CDS             complement(115..249)
                     /locus_tag="VFU90_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479201.1"
                     /translation="MKYALLSDVHANLPALDAVLGGIARSNDVVATYHGGPGRRRAVA
                     "
     gene            complement(246..725)
                     /gene="arsN2"
                     /locus_tag="VFU90_05180"
     CDS             complement(246..725)
                     /gene="arsN2"
                     /locus_tag="VFU90_05180"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEU5479202.1"
                     /translation="MTTSTNIGAASVRRATRDDLPGIERLLSSADLTTAGVADIVAAR
                     PGDFFVAEMNAMPKQIVAAAGLEVRGTTAVLRSVAVDPEWRGRGMGHELVRQIVCDAE
                     SRGIHALYLLTMTAEHYFPRLGFERIDRSSVPNEIAETAEFTSACPATAVAMKRALP"
     gene            complement(993..1328)
                     /locus_tag="VFU90_05185"
     CDS             complement(993..1328)
                     /locus_tag="VFU90_05185"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5479203.1"
                     /translation="MSSAVAVPHTTTAARLFHAVSDETRLALIHMLRDGERCVCELQD
                     ALGAAQSRLSFHLRVLKDAGLLSDRKEGRWVYYSLNADGIAELEEAAVRLKPPRRALR
                     VRHASDCCD"
     gene            complement(1372..1992)
                     /locus_tag="VFU90_05190"
     CDS             complement(1372..1992)
                     /locus_tag="VFU90_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5479204.1"
                     /translation="MTTTSEQPGTADRNTRALSLLRIAVGVLFLFFGEYKVFGTRFTL
                     GGGFEGWIHRFLADGAAYPFMIPVLRGFVLQHHVAIAFLVAYGELAIGVALVLGVLMR
                     TASVAGLVYMLALLFSSNYPGAQVAPWQYLGASLDHLVLAMCFATFATSRPFAFSLSA
                     MLARRTSVGTPVMERIREIQGGRTPVIVVPGGDRVAEAESESRPGE"
     gene            complement(2085..>3014)
                     /locus_tag="VFU90_05195"
     CDS             complement(2085..>3014)
                     /locus_tag="VFU90_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5479205.1"
                     /translation="IFSGTEWHTDIGVAGLTLSMRDSSTAADKVGSGYAHTIGAHLIA
                     GRDFGPEDEGVPPSTALVNESFAKFYFHGANPIGQLVQFDDSSIVHIVGEIADVRGQA
                     LDTSGTSGAARRIYIPYLYRSGTTKFPQPTELRLLVRTRGDPDRVLQALRRAIVATDP
                     AIAIDDLESVTHLIRFSIRDERLVAQLATGLGALALVLAAIGLFGVMSYSVGRRTNEI
                     GVRSALGAERSAIAWMVLRDGLRPVAIGIVLGFPVSIAAVRLLQHHLNDISSDAVSIA
                     VAIGVLLAAAAAAVLVPARRATRIDPISALREE"
BASE COUNT          559 a         1019 c          975 g          461 t
ORIGIN      
        1 attcacctga cggatccggc gcaggtggca gcagcgcact gatacggaca cagcacgggc
       61 acatcggaca ccgcacggac cgctggatcg caggggtccg tgccgtgtcc ttcgttaggc
      121 cacggcgcgt cgccgaccag gtcccccatg atacgtcgcg accacgtcgt tggagcgtgc
      181 tattccgccg agcaccgcgt cgagcgccgg caggttggca tgcacatccg acagcagcgc
      241 atacttcacg gcagcgctcg cttcatcgcg acagcggtgg ccggacaggc cgacgtgaac
      301 tccgccgtct cggcgatctc gttcggcacc gacgaccgat cgatgcgctc gaaccccaac
      361 cgcggaaaat agtgctcggc cgtcatcgtg agcaggtaga gggcgtgaat gccgcgcgat
      421 tcggcgtcgc acacgatctg cctaacgagc tcgtgcccca tcccgcggcc gcgccactcg
      481 gggtccacgg cgacggagcg gagcacggcc gtagtgccgc ggacctcgag acccgctgcg
      541 gccacgatct gcttgggcat ggcgttcatc tcggcgacga aaaaatctcc ggggcgcgcg
      601 gccacgatgt ccgccacgcc ggccgtcgtc aggtctgcgg agctcaacag gcgctcgatc
      661 cccggcaggt cgtcacgcgt ggcgcgccga acggacgcgg ccccgatgtt ggtcgatgtc
      721 gtcatatgca ccctcgttgc taacggtagg aacaaactac ttgcaacagt cgtcgctgcg
      781 cccgcatgcc cgcgacgatg gtttcagcgc caggtccatg cgggtttccc gtgtcgacgc
      841 ctcggcaacg tgcttggcga cggtaccgta gggctcgcgc acgacgtcgc gtatcgcgtc
      901 gagcccggcc ggctcgcttc cctcatgact ggtcatcgat cgctcccatt ttgatcaaaa
      961 aatattgata tgttagacaa gaacgacacg catcagtcgc agcaatccga agcgtgacgc
     1021 acccgcaacg cacggcgggg cggcttgagc ctgaccgccg cctcctcaag ctcggcgatg
     1081 ccatcggcgt tcagcgagta gtacacccag cgcccttcct ttcgatcgct cagcagtcct
     1141 gcgtccttga gcaccctgag gtgaaacgaa agccgcgact gcgccgcgcc gagcgcgtcc
     1201 tgtaattcgc acacacagcg ctcgccgtcc cgcagcatgt gaatgagcgc gaggcgcgtt
     1261 tcatccgaca cggcgtgaaa caaccgagcg gccgtggtgg tgtggggaac ggcaacggcg
     1321 gagctcatgc cgcaatatat caactaattt tgatccgaca aggccgacac gctattcgcc
     1381 cggtcgcgac tccgactccg cctcggccac gcggtcaccg cccggcacga cgatgactgg
     1441 ggtcctgccg ccctgaatct cgcggatccg ctccatgacc ggcgtgccga cggaggtacg
     1501 acgggccagc atggcggaga gcgagaacgc aaagggccgg ctcgtcgcaa aggtggcaaa
     1561 gcacatcgcc agcaccagat ggtctaacga ggcgccgagg tattgccacg gtgcgacctg
     1621 cgcaccggga tagttcgacg agaacagcag cgcgagcatg tagacgaggc cggcgacgct
     1681 ggcggtgcgc atcagcacgc cgagcacgag cgccacaccg atcgcaagct cgccgtacgc
     1741 gaccaggaac gcgatggcca cgtggtgctg cagcacgaac ccgcgcagca ccgggatcat
     1801 gaacggatag gcggcgccgt cggcgagaaa gcggtggatc catccctcga agccgccgcc
     1861 taacgtgaac cgcgtcccga ataccttgta ctcgccgaaa aagagaaaca acacgcccac
     1921 ggcgatgcgc aggagcgaaa gggcgcgggt gttccggtcg gcggtgccgg gctgctcgct
     1981 ggtagtcgtc acgcacctcg ggttcggagc atcgatcact gcatcctgcg ccagcgcgga
     2041 tcccgcacgt cgagtgccgc cgccggttgc cggcggcggc ttcgtcactc ctcgcggagc
     2101 gcgctgatcg gatcgatccg tgtcgcccga cgcgccggaa cgagcacagc cgccgcggcc
     2161 gccgccagca agacgccgat cgcgaccgca atggacaccg cgtcactcga gatgtcgttg
     2221 agatggtgct gcagaagtcg cacggccgca atggacaccg ggaacccgag cactatgccg
     2281 atcgcgactg ggcgcaggcc gtcccgcagc accatccacg caatcgcgga ccgttcggcg
     2341 cctaacgccg aacgcacgcc gatttcgttc gtgcgccgcc ccacgctgta gctcatcacg
     2401 ccgaacaatc caatcgccgc gagcaccagc gcgagggcgc cgagccccgt tgccagctgc
     2461 gccaccagcc gctcatcccg aatggaaaag cggatgaggt gtgtgaccga ttcgaggtcg
     2521 tcgatggcga tggccggatc ggtcgccacg atcgcgcgtc gaagcgcttg cagcacgcgg
     2581 tcggggtcgc ccctcgtgcg gacgaggagc cgcagctcgg tgggctgcgg gaacttggtc
     2641 gtgccgctcc gatagagata gggaatgtag atgcgacgcg cagcgccgga tgtgcccgac
     2701 gtgtcgagcg cctgtccgcg gacgtccgcg atttcgccga cgatgtggac gatactcgag
     2761 tcgtcgaact gcacgagctg cccgatcgga ttggcgccgt gaaaatagaa cttcgcgaac
     2821 gactcgttga cgagcgccgt cgatggcggg acgccttcgt cttccgggcc gaaatcccgg
     2881 ccggcgatga gatgcgcccc gatcgtgtgc gcgtacccgg atcccacctt gtccgccgcg
     2941 gtcgagctgt cgcgcatgct gagcgtcagg ccagcgacgc cgatgtccgt gtgccattcg
     3001 gtgccggaaa agat
//
LOCUS       DASUCJ010000436         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1166162, whole genome shotgun sequence.
ACCESSION   DASUCJ010000436 DASUCJ010000000
VERSION     DASUCJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166162"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VFU90_05200"
     CDS             complement(<1..1449)
                     /locus_tag="VFU90_05200"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEU5479206.1"
                     /translation="MDLHRFAFFHGFPAEELEQLGAAVTSAAFDADRFVFREGDPRRI
                     FALIESGSIAIEKGGDGRAVRLATLGTGEALGEGMLLEEDAPHGTSARTLEPTTIAFW
                     NALDVRAILQERPALYARLVARAARAISQRLRSADATLAGHGRMLGFADGRRRREHDL
                     LGERDVPDDAMYGIQTLRALENFPITGLPVREFPVLIAALGAVKEAAALANAELGLLP
                     TPIRDAIVAAAREIRAGRHHEHFLVDMIQGGAGTSTNMNANEVIANRALELLGHKRGE
                     YETVHPNNHVNLSQSTNDVYPTAIKLALHASIEELRDAMRTLANAFLDKGAEFAALLK
                     MGRTQLQDAVPMTLGQEFAAFGHTMLEDVDRLAETQGLLREINMGATAIGTGITAPGG
                     YAERVRAHLAAITGLPLVTAPDLVEATADTGVFVQLSGVLKRCAVKLSKICNDLRLLS
                     SGPRAGLGEINLPPMQPGSSIMPGKVNPVIPEV"
     gene            complement(1484..2398)
                     /locus_tag="VFU90_05205"
     CDS             complement(1484..2398)
                     /locus_tag="VFU90_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479207.1"
                     /translation="MANTVQADQVAQMCDERRTAIMALCQKLATDGAHYLWGAEGLRP
                     SSKAPQYFAPRAVSLDQSARGSTTFAAAQHFDQHAQRFVCAGRCFAVSLPANAIIANA
                     ASDNKLEAFIKNCIDKHLEPWNCHEKLTPRLILGTGDGEPRDYKESTEYTKQTHGHLD
                     KTLVWGESCNDKLHFDCGGFVRWVVSQVCGMSIAGISTYPTQKNALGQPMARLLTKDD
                     TVMKADILVYAGHIAFATGTPAVKYDPQTHYSVAQADCATNGVNYNVVHPQDNTSCIR
                     LSDSTLVGSVIASAVRAGMDVLSFGPRA"
     gene            2799..3893
                     /locus_tag="VFU90_05210"
     CDS             2799..3893
                     /locus_tag="VFU90_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU5479208.1"
                     /translation="MSLTRRDFIGAGAAAAAGLAMARTASASALPDSPVGRRRGAAAD
                     NRPAVVSSANGIRGVAKAREMMLQNADPLDAAIAGVNITELDPTDHSVGLGGLPNEDG
                     VVQLDASCMHGPTRRAGAVGAIEDIATPSLVAKAVMDYTDHIFLVGAGAQKFALEMGF
                     KQQNLLTDEARQIWLKWKANHDPNDAWLDHDDDVKVQFTHGTINMNAVTAGGDIGSVT
                     TTSGMSYKIPGRVGDSAIVGAGQYCDNDVGASGSTGRGEANIKIAGAFFVVEQMRNGA
                     SPEEACLRALQRAIQMTEARLVDERKRPRYDLTYYAIAKDGRYGSANIYSGLQFAVAD
                     ARGARLEPCAYLFKESERPRGPVPMHALSR"
     gene            3912..5531
                     /locus_tag="VFU90_05215"
     CDS             3912..5531
                     /locus_tag="VFU90_05215"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479209.1"
                     /translation="MRRGVRRLTVAAIAAVLAACATAHPAPNAERASGPRFHIAYAIA
                     MPDTIGHLYEIQIDIGGSLGDTLRLQMPVWSPGRYARMDFARNVRDESVQGANGRPVR
                     FDRENGSLWRIYPAGATHVTMRYRVFADNLSGTFSVLDTAHANWNGASLFMYVVGHKP
                     DSVSLAVTPPNGWHIINGASTSPDQRQFRFANYDEMIDTPTEVAKHFDVDSFTVDNRL
                     YRVVVHHNGSQNGYHQGFVSAVEKIVRYENTVVAPPPLTMYTFLFNVGYDGSDGMEHL
                     YSTQIQTRAPWTDSSAVMATISDAAHEYFHTWNVKRIRPMLLGPFDYSSERYEPSLWV
                     AEGWTQYYGEIGLLRAGIVSKDDYYQTLAHVIDANLESPGRKVVSARMASFLAPFWDG
                     AATPMRVDRGSFFSYYTKGEGLALLLDLDIRERTHNARSLDDALRALKRMTWDAPNAS
                     YYLQGRGYTEDDVVRAASEAAGADLTPWFDRYVGGVEDPPFAASLAEAGLRLQSQPGP
                     RGTEYTVVEDPNATADQLRVRHGWLTGTVGP"
     gene            complement(5551..>5925)
                     /locus_tag="VFU90_05220"
     CDS             complement(5551..>5925)
                     /locus_tag="VFU90_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU5479210.1"
                     /translation="GHAGQGDGEVDQTAIETSLTGRFQVIVRKDLHFEWPRAETPTEL
                     MTMGMDKDLTQATRVAVQQMVDLLGEQLGLPHDKAYALASVAADLKIAELVDGNMCVY
                     MTIPKKIAGPTFKPEAATGAGP"
BASE COUNT          978 a         1918 c         2013 g         1016 t
ORIGIN      
        1 gacttcgggg atgaccgggt tcactttgcc cggcatgatg gatgagccgg gttgcatggg
       61 aggcaaattg atttcgccca acccggcgcg cgggcccgag ctgagcagtc tgaggtcgtt
      121 gcagatcttc gagagcttga ccgcgcagcg cttgagcact ccagacagct gcacgaacac
      181 gccagtgtcg gcggtggctt cgacgaggtc gggtgcggtg actaacggaa ggccggtgat
      241 cgccgcgagg tgcgcccgca cgcgttcggc gtatccgccc ggcgcggtga tcccggtgcc
      301 gatggcggtc gcgcccatgt tgatctcgcg gagcaggccc tgcgtttccg cgaggcggtc
      361 gacgtcctcg agcatcgtat gtccgaacgc ggcgaactcc tggcccagcg tcatggggac
      421 ggcgtcctgc aactgggtgc ggcccatctt gaggagcgcg gcgaactcgg cgcctttgtc
      481 gaggaacgcg ttggcgagcg tgcgcatcgc atcgcgcaat tcttcgatgc ttgcgtgcag
      541 cgcaagtttg atcgcggttg gatagacgtc gttcgtcgac tgcgagaggt tgacgtgatt
      601 gttggggtgg accgtctcgt actcgccgcg cttgtggccg aggagctcga gcgcgcgatt
      661 ggcgatgacc tcgttggcgt tcatgttggt cgaggtgccc gcgccgccct ggatcatgtc
      721 gacgaggaaa tgttcgtggt ggcggccggc gcggatttct cgcgcggcgg cgacgatggc
      781 atcgcggatt ggagtcggca ggaggcccag ctccgcgttg gcgagcgctg ccgcttcttt
      841 gacggcgccg agcgcggcga tgaggacggg aaattcgcgc accggcaggc cggtgatcgg
      901 gaagttctcg agggcgcgca gcgtctggat gccgtacatg gcatcgtcgg ggacgtcgcg
      961 ctcgcctaac agatcgtgct cgcgccgccg gcgtccgtcg gcgaagccca gcatgcggcc
     1021 gtggccggcg agggtcgcgt cggcggagcg gaggcgctgg gagatggcgc gggcggcgcg
     1081 ggcgacgagg cgtgcataga gggccggccg ttcttgcagg atcgcgcgga cgtcgagcgc
     1141 gttccagaac gcgatggtcg tcggttcgag cgtgcgcgca ctcgtcccgt ggggcgcgtc
     1201 ttcctcgagc agcatgccct cgccgagggc ttcgccggtg cctaacgttg ccaggcggac
     1261 ggcgcgtccg tcgccgcctt tctcgatggc gatgctgccg ctctcgatga gggcgaagat
     1321 acggcgcggg tcgccttcgc ggaacacgaa gcggtcggcg tcgaaggcgg cggacgttac
     1381 ggcggcgccg agctgttcga gctcctcggc gggaaagccg tgaaaaaacg cgaagcggtg
     1441 gaggtccaca ggtcgttagg ccgtgcaggc ggaaggacga agctcaggcg cgcgggccga
     1501 aagaaaggac atccatgcct gcgcgaacgg cgcttgcgat tacgctgccg accagcgtcg
     1561 agtcggacag acgaatacac gacgtgttgt cttgtggatg cacgacgttg tagttgacgc
     1621 cgttcgtcgc gcagtcggcc tgcgcaacgc tgtagtgcgt ttgcgggtcg tatttcaccg
     1681 ccggcgtgcc cgtcgcaaac gcaatgtggc cggcgtagac gaggatgtcg gccttcatca
     1741 cggtatcatc tttcgtcagc aatctcgcca tgggctggcc gagcgcgttc ttctgcgtgg
     1801 gatacgtgct gatgcccgca atcgacatcc cgcagacctg cgagacgacc caccggacga
     1861 atccgccgca gtcgaagtgc agcttgtcgt tacaagactc gccccagacg agcgttttgt
     1921 ccaagtgccc gtgcgtctgc ttggtgtact cggtgctttc cttgtagtcc cggggttcgc
     1981 catctccggt acccaggatc aaccgcggcg tgagtttctc gtggcagttc cacggctcga
     2041 gatgtttgtc gatgcagttc ttgatgaatg cctccagctt gttgtcagac gctgcgttgg
     2101 cgatgatcgc gttcgccggc aacgacaccg cgaagcagcg gcctgcgcat acgaatcgtt
     2161 gcgcgtgctg atcgaagtgc tgtgcggccg cgaacgtcgt cgatccgcgc gccgattggt
     2221 cgagactgac cgcccgagga gcgaagtatt gtggcgcctt actggacggc cgcaggcctt
     2281 cggcgcccca gaggtagtgc gcgccgtcgg tggccaactt ctggcacagc gccatgatcg
     2341 cagtgcgccg ctcatcacac atctgcgcca cttggtcggc ctggacggtg ttcgccattg
     2401 agactccttc gatcaggcgt cggatgatga gattcgagcg ctttcgcaac gtcggaagat
     2461 tacgttcgcg gccgacaacg gaaagagcga gcggtagagg tacagtttgg tcagtacggg
     2521 gctgagactc atcagtcgcg acccgagtgg cgcgaggcac ccttctcgcc cgtggtgggc
     2581 ttcctcgctt tgatgatatt tccagagcga ggaagctcac cacctcgcat ctattccggc
     2641 actcgtcccc gcgccggcac cccaaactgc accggtaccg agcgagctgg cgcctggtgt
     2701 ccctcgaagc gtgggatggc gctgttgcgg ttgcttcgcg cggggccgcg gatcaaattc
     2761 gcggcgttcg cagttccctt ggctcgagga ggcaccgatt gtctctcacc cgccgtgatt
     2821 tcattggcgc cggcgccgcc gctgccgccg gtctcgcgat ggcccggacg gcgagcgcgt
     2881 ctgcgttgcc ggattcgccg gtcgggcgcc ggcgcggcgc ggccgccgac aaccgtcccg
     2941 ccgtcgtctc gagcgccaac ggcattcgcg gcgtcgccaa ggcgcgcgag atgatgctcc
     3001 agaacgccga tccgttagac gcggcgatcg cgggcgtcaa catcacggag ctcgatccga
     3061 ccgaccactc ggtggggctc ggcggtctgc ctaacgaaga cggggtcgtc cagctggatg
     3121 cgtcgtgcat gcacgggccg acgcggcgcg ccggcgccgt cggcgcgatc gaggacatcg
     3181 cgacgccgtc gctcgtcgcc aaggcggtga tggactacac cgaccacatc tttctcgtcg
     3241 gcgccggcgc ccagaaattc gccctcgaga tgggcttcaa gcagcagaac ctgctcaccg
     3301 atgaagcgcg gcagatctgg ctcaagtgga aagccaacca cgatccgaac gatgcctggc
     3361 tcgaccacga cgacgacgtg aaggtgcaat tcacgcacgg caccatcaac atgaacgccg
     3421 tcaccgcggg cggcgacatc ggcagcgtga cgacgacgag cgggatgtcc tacaagattc
     3481 ccggccgcgt cggcgacagc gccatcgtcg gcgccggcca gtactgcgac aacgatgtcg
     3541 gagcgtccgg gagcacggga cgcggggaag ccaacatcaa gatcgctggc gcgttcttcg
     3601 tcgtcgagca gatgcggaac ggcgcatcgc cggaagaggc atgcctgcgg gcgctgcagc
     3661 gcgccatcca gatgaccgag gcgcgcctgg tcgatgagcg caagcggccg cgctacgacc
     3721 tgacgtatta cgcgatcgcg aaggacggcc ggtacggcag cgcgaacatc tattcggggc
     3781 tgcagttcgc ggtggccgac gcgcgcggcg cgcggctgga accgtgcgcc tacctgttca
     3841 aggagagcga gcgcccgcgc ggcccggtgc cgatgcacgc gctgtcgcgc tgacgatcaa
     3901 tgcctaacca catgcgccgg ggcgtccggc gcctgaccgt cgccgcgatc gccgcggtcc
     3961 tcgcggcctg cgccaccgcg caccccgcgc ccaacgcgga gcgcgcgtcc ggcccgcgct
     4021 tccacatcgc gtacgccatc gcgatgcccg acacgatcgg gcacttgtac gagatccaga
     4081 tcgacatcgg cggctcgtta ggcgatacgc tccggctcca gatgcccgtc tggtcgccgg
     4141 gccgctatgc gcgcatggac ttcgcgcgca acgtgcgcga cgagtcggtg cagggtgcga
     4201 acggccgacc cgtacgcttc gatcgcgaaa acggctcgct ctggcgcatc tatcccgccg
     4261 gcgcgacgca cgtcaccatg cgctaccgcg tgttcgccga caacctgtcg ggcacgttca
     4321 gcgtcctcga caccgcgcac gccaactgga acggcgcgtc gctgttcatg tacgtggtgg
     4381 gacacaagcc ggactcggtc tcgctcgccg tgaccccgcc caacggatgg cacatcatca
     4441 acggcgccag cacgtcgccc gaccagcgcc aattccgctt cgcgaactac gacgagatga
     4501 tcgacacgcc caccgaagtg gccaagcact tcgacgtcga cagcttcacc gtggacaacc
     4561 ggctctatcg cgtcgtcgtg caccacaacg ggtcgcagaa cggctatcac cagggcttcg
     4621 tcagcgccgt cgagaaaatc gtgcgctacg agaacaccgt cgtcgcgccg ccgccgctca
     4681 ccatgtacac cttcctgttc aacgtgggtt acgacggcag cgacgggatg gagcatctgt
     4741 actcgacgca aatccaaacg cgcgcgccat ggaccgattc gtcggccgtg atggcgacga
     4801 tctcagacgc ggcgcacgag tatttccaca cgtggaacgt gaagcgcatc cggccgatgc
     4861 tgctcgggcc gttcgactat tcgtccgagc ggtatgaacc gagcctgtgg gtggcggagg
     4921 gatggacgca gtactacggc gaaatcggac tgctgcgcgc cgggatcgtg agcaaagatg
     4981 actactacca gacgctcgcc cacgtcatcg acgcaaacct ggagtccccg ggccgcaaag
     5041 tcgtctcggc gcgcatggcg tcgtttctgg cgccgttctg ggatggcgcg gcgacgccga
     5101 tgcgcgtcga ccgcgggagc ttcttctcgt actacaccaa aggcgaaggc ctcgcgctgc
     5161 tgctcgacct cgacatccga gaacggacgc acaacgcgag atcgctggac gatgcgctgc
     5221 gcgcgctcaa gcgcatgacc tgggacgcgc ctaacgcaag ctactacctg caaggacgcg
     5281 gctacaccga agacgatgtg gtgcgcgccg cgagcgaggc ggccggcgcg gatctgacgc
     5341 cgtggttcga ccgatacgtg ggcggtgtcg aagatccgcc gttcgcggcg tcgctcgccg
     5401 aggccggctt gcgcttgcag tcacaacccg ggccgcgcgg caccgagtac acggtggtgg
     5461 aagatccgaa cgcaaccgcc gatcagctcc gcgtgcggca cggatggttg accggaacgg
     5521 tcggtccctg agcgtccatg cggtgccgca tcaggggccg gcgcccgtcg ctgcctcggg
     5581 cttgaacgtc ggtccggcga tcttcttcgg gatagtcatg tacacgcaca tgttgccgtc
     5641 caccagctcg gcgatcttga gatccgcggc gacgctcgcc agcgcgtagg ccttgtcgtg
     5701 cggcaggcct aactgctcgc ccaacaaatc gaccatctgc tgtacggcga cgcgcgtcgc
     5761 ctgcgtcaga tccttgtcca tgcccatcgt catgagctcg gtgggcgtct cggcgcgggg
     5821 ccactcgaag tggagatcct tgcgcacgat cacctggaac cggccggtga gcgacgtctc
     5881 gatcgccgtc tggtccacct cgccgtcgcc ctgacccgcg tggcc
//
LOCUS       DASUCJ010000437         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_279726, whole genome shotgun sequence.
ACCESSION   DASUCJ010000437 DASUCJ010000000
VERSION     DASUCJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279726"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="guaA"
                     /locus_tag="VFU90_05225"
     CDS             <1..1216
                     /gene="guaA"
                     /locus_tag="VFU90_05225"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5479211.1"
                     /translation="VVNRGTPLFDDVPVHSRVWMSHGDSVIKMPDGFVSLASTQRCNV
                     AAMGDAERKIYGVQFHPEVVHSDNGRLILSNFLYKVAGLADEWRMESFVEGAIAAIRA
                     KVGSDRVICALSGGVDSAVAATLVSHAIGEQLICVFVDHGLLRKNEAQEVVAAFRDVL
                     HLNVRVVDAGKKFLKKLKGVRDPEKKRIIIGHQFIRVFEKEAALIPGVKYLVQGTLYP
                     DVIESKTPESKAGHKIKSHHNVGGLPARMNLALIEPLRALFKDEVRELGRVLGLPGEI
                     VERQPFPGPGLAVRIIDDVTKDRLEILREADAIVREEIDAAVLDPHPWQYFAVLTPVK
                     SVGVMGDGRTYANLVAIRAITSEDGMTADWARLPHDLLERISSRIVNEVPGVNRVAYD
                     VTTKPPATVEWE"
     gene            1221..2504
                     /gene="purD"
                     /locus_tag="VFU90_05230"
     CDS             1221..2504
                     /gene="purD"
                     /locus_tag="VFU90_05230"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5479212.1"
                     /translation="MRILVIGNGAREDALSWRLAQSPSCDAIFATPGNAGTASRGENW
                     GIAATYGRAIVERCQAERVDLAVIGPETAIAAGVGDRLREAGIAVFGPNRSSGRLESS
                     KVFAKRFMQRHGVPTARGVVAHNLDGALKTLEEWRGAVVVKADGLAAGKGVVVTANPD
                     EARAVLQEWYGANKVPGGGSDVLLEEKLDGREVSVFAIADGRAMAPFAAACDYKRAGD
                     GDTGPNTGGMGAYSPPSGFPEDLDDRVRERILAPVLRGLLAEGEEYVGVLYCGLMWTA
                     DGPSVIEFNARFGDPETQVLMPRVRGDFAALLRSAADGAMDLSLAALSAQSCAGVVLA
                     TSDYPRSSTPLAGLTADVSLPEGCQAFWGGSRRVDGTVEAGGGRVLTVTALGDELDEA
                     RRRAYEAVRALAGRLGSSALTYRTDIAAVGPKVPQ"
     gene            2593..2946
                     /locus_tag="VFU90_05235"
     CDS             2593..2946
                     /locus_tag="VFU90_05235"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU5479213.1"
                     /translation="MAMLLQATHETDSGRIDARLLREPTGFSLAEIARFIGVKVQTLK
                     SNSSAPSAQPGLAKLVNAWEILQTIFPSDAAIRGWLHHPIRRFKGRTPKWLLESEGID
                     AFEALVEEMAAGSHG"
     gene            2987..3481
                     /locus_tag="VFU90_05240"
     CDS             2987..3481
                     /locus_tag="VFU90_05240"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU5479214.1"
                     /translation="MKAPSLQFSGIVARCAFEKYRATATSPAGAARRGGRYTPIGTNA
                     LYTSIKRTTAFSEFTQYYTDDDPLPVTCVFTLMVFVRRMVDLTDPATVAALGTTPEEL
                     GGVRIPGRPFFAQAIGEVANGLGIHALLVWSAQEPAEKNLVVLPDNAPPMTATCLVVT
                     ALKS"
BASE COUNT          646 a         1121 c         1329 g          612 t
ORIGIN      
        1 ggtcgtcaat cgcggcacgc cgctgttcga cgacgtgccg gttcattcgc gggtgtggat
       61 gtcgcacggg gattcggtta tcaaaatgcc ggacggcttc gtttcgctgg cgtcgacgca
      121 gcgctgcaac gtggccgcga tgggggacgc cgagcggaag atctacggcg tgcagttcca
      181 tccggaagtc gtccactccg acaacgggcg cctgatcctc agcaacttcc tgtataaggt
      241 ggcgggtctc gcggacgagt ggcggatgga gtcgttcgtc gagggcgcga tcgccgcgat
      301 ccgcgcgaag gtgggcagcg accgggtgat ttgcgcgctg tcgggcgggg tggactcggc
      361 ggtcgcggcc acgctggtgt cgcacgccat cggcgagcag ctgatctgcg tcttcgtcga
      421 tcacggcttg ttgcgcaaga acgaagccca ggaggtcgtg gcggcgttcc gcgacgtgct
      481 gcatctcaac gtgcgggtcg tcgacgccgg taagaagttc ttgaaaaagc tcaaaggggt
      541 gcgggatccc gagaaaaagc ggatcatcat cggccatcag ttcattcgcg tcttcgaaaa
      601 agaagcggcg ctcattccgg gagtcaagta cctggtgcag ggaacgctct accccgacgt
      661 catcgaatcg aaaacccccg agagcaaggc cggtcacaag atcaaatcgc accataacgt
      721 cggcggtttg ccggcgcgca tgaatctcgc gctgatcgaa cccttgcgcg cgctgttcaa
      781 ggacgaggta cgcgagctag gccgggtgct cgggcttccc ggcgagatcg tcgagcgcca
      841 gccgttcccc ggcccggggc tcgcggtgcg aatcatagac gacgtcacca aagatcgctt
      901 ggagatcctc cgcgaggcgg acgccatcgt ccgcgaggaa atcgacgccg cggtgctcga
      961 tccgcatcct tggcagtact tcgccgtact gacgccggtc aagagcgtcg gcgtgatggg
     1021 cgacggacgc acgtacgcga atttggtcgc cattcgggcg atcacgagcg aagacgggat
     1081 gacggccgac tgggcgcggc ttccgcacga cttgctggaa cgcatctcgt cgcgaatcgt
     1141 caacgaagtt cccggcgtga accgggtcgc gtacgacgtc acgacgaaac cgccggccac
     1201 cgtcgagtgg gagtagcgcg ttgcgcatcc tcgtaatcgg caacggcgcg cgcgaagacg
     1261 cgctgtcgtg gcggctggcg caatcgccgt cgtgcgatgc gatcttcgca acgccgggga
     1321 atgcgggcac cgcgtcgcgc ggcgagaatt ggggaatcgc cgcaacctac ggacgggcca
     1381 tcgtcgagcg ctgccaggcc gagcgcgtcg atcttgcggt tatcgggccg gagacggcga
     1441 tcgcggccgg ggtgggcgac cgcctgcgtg aggcgggaat tgcggtattc ggccccaatc
     1501 gctcgagcgg gcggctggag tcgagcaagg tgttcgccaa gcgcttcatg cagcggcacg
     1561 gcgttccgac ggcgcgcggc gtggtggcgc acaatctcga cggcgcgctc aaaacgctcg
     1621 aagagtggcg cggcgcggtg gtggtcaaag cggacggtct ggccgccggt aagggcgtgg
     1681 tggtgacggc gaatccggat gaagctcgcg cggtgctgca ggagtggtac ggtgcgaaca
     1741 aagtgccggg cggcggcagc gacgtgctgc tcgaggaaaa gctcgacggg agagaagtca
     1801 gcgtgttcgc gatagccgac ggacgcgcga tggcgccgtt cgccgccgcg tgcgactaca
     1861 agcgcgccgg cgacggcgat accggtccga ataccggagg tatgggggcg tactcgccgc
     1921 cgagcggttt cccggaggat ttggacgatc gcgtccgcga gcggattctc gccccggtgc
     1981 tgcgcggctt gctcgcggaa ggcgaagaat acgtcggcgt tttgtactgc ggactgatgt
     2041 ggacggcaga cggaccgagc gtcatcgagt tcaacgcgcg cttcggcgat cccgagacgc
     2101 aagtgctgat gccgcgcgtg cgcggcgatt tcgccgcgct gctcagatcg gcagccgacg
     2161 gtgcgatgga cttgtcgctg gcggcgcttt cggcgcaaag ctgcgccggg gtggtgctgg
     2221 ccaccagcga ttacccgcgc agcagcacgc cgctcgcggg gctgaccgcg gacgtctcgc
     2281 tccccgaggg ctgccaggcg ttttggggcg gctcccgccg cgtcgatggt acggtggaag
     2341 ccggcggggg gcgggtgctg accgttaccg cgctcggcga cgagctcgac gaggcgcgac
     2401 ggcgggcgta cgaggccgtg cgcgcgctgg cgggcaggct cggctcgagc gccctgacct
     2461 accgcaccga catcgccgcg gttggtccga aggtaccgca ataggatgca ctctaggtgg
     2521 taagaagggt gtattttcgt agacatattg ggtgcattac ggtataatcg ccggtaggag
     2581 gactcccttg ccatggccat gctcctgcag gcgacccacg agaccgacag cggccggatc
     2641 gacgcccggc tgctgcgcga gccgaccgga ttttcgctgg ccgagatcgc ccgttttatc
     2701 ggggtaaaag tgcaaacgct caaatccaac tccagcgcgc ccagcgccca gccggggctc
     2761 gccaaactcg tcaacgcgtg ggagatcctg cagacgatct ttccgtcgga cgccgcgatt
     2821 cgaggctggc tgcaccaccc gatccgccgc ttcaaagggc gtacgcccaa gtggctgctg
     2881 gagagcgaag gcatcgacgc ctttgaagcg ctggtagagg agatggccgc cggaagccat
     2941 ggctaggagc gcgcccctga atagggtcaa ggtacagacg gcgctgatga aggcgccgtc
     3001 gctgcaattt tccggaatcg tcgcccggtg cgccttcgag aagtatcgag cgacggccac
     3061 ctcgccggcc ggtgcggcgc gccggggcgg ccggtatacg ccgataggca cgaacgcgct
     3121 ctatacgtcg atcaagcgaa cgaccgcgtt ttccgagttc acgcagtact ataccgacga
     3181 cgatccgctc ccggtgacgt gcgtcttcac gttgatggtg ttcgtgcgcc gaatggtcga
     3241 tctgacggat cccgcgaccg tcgcggcgct ggggacgacg ccggaggaac tgggcggcgt
     3301 gcggattccc ggccggccgt ttttcgcgca agcgatcggg gaagtcgcca acggcttggg
     3361 cattcacgcg ctgctggtgt ggagcgcaca ggagccggcc gaaaagaacc tggtcgtctt
     3421 gccggacaac gcaccgccga tgacggcgac ctgtctggtg gtgacggccc taaagtcgta
     3481 gatcttcgcg acctcttccc gattcggcgc taccatgcga gagagcaccg aatcggaggc
     3541 gtgttatggc gaagtttacg attatcgaag ggcatccgga tccgtcggcg agccggctca
     3601 accgggcgct ggcggaccgc tacgcggaag cggccgtcgc cgcgggacac gaagtgcggc
     3661 gaatcgacgt ggcgcgaatg gagattccgc tgctgcgcag cgccgacg
//
LOCUS       DASUCJ010000438         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_279732, whole genome shotgun sequence.
ACCESSION   DASUCJ010000438 DASUCJ010000000
VERSION     DASUCJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279732"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFU90_05245"
     CDS             <1..186
                     /locus_tag="VFU90_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479215.1"
                     /translation="KGRRRTIRKTTPLALTMPSEFKAQNGASFKQSTRIAVQGCAKGK
                     AGKGKKAKKGKGGKHKK"
     gene            404..1210
                     /locus_tag="VFU90_05250"
     CDS             404..1210
                     /locus_tag="VFU90_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479216.1"
                     /translation="MQDGLNNPNTDNRGRLAPFADHQAIGSRKQCKGAVREMQRLKML
                     GLAVVAVLALGAAVTAAASAEVSFLPEGTAAEPVTGSGTAGPGTMQTLSGQSIQCAAL
                     KGTGEVTSKQSGHAVLELEGCHAVVGGVEVKCADLSHEDASGIVKIEGTFHLRMGLTG
                     QPLGIIAALVNPDVHFLCSITLWVALGCIAGEVTQLETLSNEMEGSLRQNAGENEILS
                     IDNEAETAMEECTLLVKKGTGTEETAGLEGHAKIGNFKKGTTSVTALVHA"
     gene            complement(1432..2085)
                     /locus_tag="VFU90_05255"
     CDS             complement(1432..2085)
                     /locus_tag="VFU90_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479217.1"
                     /translation="MHASGTAHRKAALWLAATVGVLALSLASAALSQAEESNPNNYNC
                     LGGLSAGTPVPGSEEQQVQYSFYCNGPITGYQVQSQIPVTGVSSPPIVTNVEKRELAD
                     TFSCSGEIPGWAANCVGSTANGYETVTGQFAIGAKLCAEPRVDALLTVAYAYLEKGKV
                     TQAISGPYDLGRPKGCKPDWYSHWNRFNPQPLTQKKHPAKKGAKRKSSSKQRWSKKK"
     gene            complement(2145..2687)
                     /locus_tag="VFU90_05260"
     CDS             complement(2145..2687)
                     /locus_tag="VFU90_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479218.1"
                     /translation="MWLVELSGSSFAEATSNAAAAPYITGQLLRQGTLLSGWSALGAS
                     AFASDAALAEHRATVGGAPPALHTIVQPPCAEGAAEASCAPGTPAGVAAADRFAQATL
                     ATITATSTYAEHGLVVVTYATVADPSTAELPAGAAQATLGATPPAGALLVSPFAKAGA
                     RSAVIFNSTSPTRSLEALLH"
BASE COUNT          494 a          900 c          953 g          480 t
ORIGIN      
        1 aagggcaggc gcaggacgat cagaaagacc acgccgctgg ccctgacgat gccgagcgaa
       61 ttcaaggcgc agaacggggc cagcttcaag cagagcacga ggatcgcggt gcaggggtgc
      121 gcgaagggca aggcgggcaa gggcaagaag gcgaagaagg gcaagggcgg gaagcacaag
      181 aagtagccag gcgcttcggc gctctggccg gagcgggtgc gccaagcgcg ccagcgcagc
      241 gcccgaaggt cgcgccttcg acccaggggc tcgatggggc cttgagctga agtcacgcca
      301 gccgcgcccg cgcacaactg acaagaccgt caccacttgt ctgcagtcgg agacgatcgc
      361 ggccgatccc gcgctagctc gtcgcggcgt gccatattcg gctatgcaag acggcctgaa
      421 taatcccaat accgacaacc gaggtcgtct tgcgccgttc gccgatcatc aggcgatcgg
      481 ctctcgcaaa caatgcaaag gagcagtgag agagatgcaa cgactgaaga tgctgggtct
      541 cgcggtggtc gctgtgcttg cgctcggcgc cgccgtcacg gctgcggcgt ccgctgaagt
      601 gagcttctta ccggaaggga cggctgcgga accggtgaca ggtagcggga cggccgggcc
      661 cggaaccatg caaacattat cgggacagag cattcaatgc gcagcactga aaggcacggg
      721 tgaagtcacc tcgaaacagt cagggcacgc tgtgctcgaa cttgaagggt gtcacgctgt
      781 tgtcggcggc gtggaagtca aatgcgccga tttatctcac gaagacgcat cgggaatcgt
      841 gaagatcgaa ggtacattcc atctacggat gggcttaacg gggcagccgt tagggatcat
      901 cgcagcgtta gtcaaccctg acgtgcattt cttatgttcg atcacgttat gggtggcgtt
      961 aggatgcatc gccggggaag tcacgcagct agaaacctta tcgaatgaaa tggaagggtc
     1021 gctacggcaa aacgcaggcg agaacgaaat cttatcgatc gacaacgaag ccgaaacggc
     1081 gatggaagag tgcacgctat tagttaaaaa aggtacaggc accgaggaaa ctgcgggtct
     1141 ggaagggcac gcgaaaatag gcaacttcaa aaaaggcacg acgtcagtca cggctttagt
     1201 tcatgcataa cgacagacga tgacgcgtct gaatcgatga acaccacaag cgggggcgcg
     1261 gcgcgccccc gcttgtggtg tgcaagtcgt ctgcgtgatc caagacgaac gatcagtccc
     1321 cgcgatctgc atcgtgcccg ggccgcgccg tgcgaaatgc ggcgcgcgcc gacgcgcgcg
     1381 gacgatccct cagagccgcc cgcgcgacgc cgcccgcgtg tcctcgcgtc cctacttctt
     1441 cttgctccac ctctgcttcg agctcgactt ccgcttggca cccttcttcg ccggatgctt
     1501 cttctgcgtc agcggctgcg ggttgaagcg gttccagtgc gagtaccagt ccggcttgca
     1561 gcctttcggc cggccgaggt catacggacc ggagatcgcc tgcgtgactt tgcctttttc
     1621 gaggtacgcg tacgcgacgg tcagcagcgc gtcgacgcgc ggctcggcgc acagcttcgc
     1681 gccgatcgcg aactggcccg tgaccgtctc gtagccgttc gccgtcgagc cgacgcagtt
     1741 cgccgcccag cccgggattt cacccgagca cgagaacgtg tcggccagtt ccctcttttc
     1801 gacgttcgtc acgatcggcg ggctcgacac tcccgtcacc gggatctgcg actgcacctg
     1861 gtagccggtg atcgggccgt tgcagtagaa gctgtactga acctgctgtt cttcgcttcc
     1921 gggcaccggc gtgccggcgg acaggccgcc gaggcagttg tagttgttcg ggttcgattc
     1981 ttcggcctga ctcagcgccg cgctcgcaag cgacagggcg agcacgccca cggtcgcggc
     2041 cagccacagc gcggcctttc ggtgggcggt tccagatgcg tgcaacaggt tctccgtttc
     2101 gtttcgtggg acgtggatct ggttgtgggt cggtgcttcg tgcgtcagtg caggagcgct
     2161 tccaggctcc gcgtgggtga ggtcgagttg aagatgactg ccgatcgtgc gccggccttg
     2221 gcgaacggcg agacgagcag tgcgcccgcc ggcggcgtcg ccccgagcgt cgcctgagcc
     2281 gcgcccgccg gcagttcggc cgtgctcggg tccgcgaccg tcgcgtacgt cacgacgacg
     2341 agcccgtgtt ccgcgtacgt gctcgtcgcc gtgatcgtcg ccagcgtcgc ctgcgcgaac
     2401 cggtcggccg ccgcgacgcc cgccggcgtg cccggcgcgc acgacgcttc cgccgcgcct
     2461 tccgcgcacg gcggctgcac gatcgtgtgc agcgccggcg gcgcgccgcc gaccgtcgcg
     2521 cggtgttcgg ccagcgccgc gtcgctcgcg aacgcgctcg cgccgagcgc cgaccagccg
     2581 ctcagcagcg tgccctgccg cagcagctgg cccgtgatgt acggcgccgc cgccgcgttc
     2641 gacgtcgctt cggcgaacga cgagcccgac agctcgacca gccacacgct cgtgatcggc
     2701 ggcagcgtcg cgccggcgct cggtttcctc ggcgtcgacg gtttcgtttg ggattcgctt
     2761 gccggcgtcg gcgtcgtgct ggccggcgcc ggcgtactcg cggccgtcgt cgtcggcgcg
     2821 cggctcg
//
LOCUS       DASUCJ010000439         1493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_982684, whole genome shotgun sequence.
ACCESSION   DASUCJ010000439 DASUCJ010000000
VERSION     DASUCJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982684"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1493)
                     /locus_tag="VFU90_05265"
     CDS             complement(<1..>1493)
                     /locus_tag="VFU90_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5479219.1"
                     /translation="SQHDEQLPSYNAYSADGDVTGPLVYVNYGVPADYDELARHGISV
                     KGAIVIARYGGSWRGIKPKVAAEHGAIGCLIYSDPRDDGYAAGDVFPKGPFRPRDGVQ
                     RGSVADMPLYSGDPLTPGVGAVPGAKRLTLAEAKTLTKIPVLPISYGDAQPLLAAMTG
                     PVAPAAWRGALPVTYHLGPGTGRVHLRVQSNWSTKPMYDVIATLRGSTYPDEWVIRGN
                     HHDGWVNGAGDPLSGQSALLDEARALGDLVRQGWHPKRTIVYAAWDGEEPGLLGSTEW
                     VETHADELRQHAAVYINSDGNDRGLLNMGGSHSLEQFANGIARDIQDPETNMSVWQRL
                     RLSEISDAKNSDERNELRTRPDLRIAPLGSGTDFTGFLDHLGIATLDLSYGGEDPQGI
                     YHSIYDDFYWYTHFSDKDFVYGRALSQTIGSSVMRLADADILPFDFTDFADTVHKYTK
                     GLEKLLADKQDEARERDKELDEGVFKATFDPRRPTVAPPREEVPPHL"
BASE COUNT          243 a          488 c          484 g          278 t
ORIGIN      
        1 cagatgcgga ggcacttctt cccgcggagg cgcaaccgtc ggccgccgtg gatcaaacgt
       61 cgccttgaac actccttcat cgagttcttt atcgcgctcc cgcgcttcgt cctgtttgtc
      121 ggccaacaac ttttcgagcc ctttcgtgta tttgtgcacg gtgtcggcga aatcggtgaa
      181 atcaaacggc agaatgtccg catcggcaag gcgcatcacc gagctgccga tcgtctgcga
      241 caatgctcgt ccatagacga aatccttgtc ggagaaatgc gtgtaccagt agaaatcgtc
      301 gtagatggag tgatagatgc cctgcggatc ttctcctcca tagctcaggt cgagcgtggc
      361 gatgccgaga tgatcgagga atcccgtgaa atcggtgccg gagccgagcg gggcaatccg
      421 cagatcgggc cgggtccgca gttcgttgcg ttcgtcagaa ttcttcgcat cagaaatttc
      481 tgacagacgc agccgctgcc agacactcat attcgtttcc ggatcttgaa tgtcgcgcgc
      541 aatgccgttg gcaaattgtt cgagcgagtg cgatccgccc atgttgagca ggccgcggtc
      601 gttgccgtcg gagttgatgt agaccgcggc atgctgccgc agttcgtcgg cgtgcgtctc
      661 gacccattcg gtgctgccta acagaccggg ctcctcgccg tcccaggccg cgtagacgat
      721 ggtgcgcttc gggtgccagc cctgccgcac caggtcgcct aacgcacggg cctcgtcgag
      781 cagcgccgac tggccggaga gcggatcgcc cgcgccgttc acccagccgt cgtggtggtt
      841 gccgcggatc acccactcgt cggggtacgt cgatcctctg agcgtggcga tcacgtcgta
      901 catcggcttg gtcgaccagt tcgactggac gcgcagatgg acacgccccg tgccgggccc
      961 caagtggtac gtcacgggca gcgcgccgcg ccaggcggcc ggcgccaccg gacccgtcat
     1021 ggcggcgagc aggggctgcg cgtcgccgta cgagatgggc agcaccggaa tcttcgtgag
     1081 cgtcttcgct tcggcgagcg ttaggcgctt ggcgccgggc accgcgccca cgccgggcgt
     1141 gagcgggtcc cccgagtaga gcggcatgtc ggccacgctg ccgcgctgca cgccatcgcg
     1201 cggacggaac gggcccttcg ggaacacgtc tcccgccgcg tagccgtcgt cgcgcggatc
     1261 ggagtagatg agacagccga tcgcaccgtg ctcggccgcg accttcggct tgatgccgcg
     1321 ccacgagccg ccgtaccgcg cgatcacgat cgcgcccttc accgaaatgc cgtgtcgcgc
     1381 gagctcgtcg tagtccgccg gcacgccgta gttcacgtac acgagcggac cggtgacgtc
     1441 gccgtcggcc gagtacgcgt tgtagctcgg cagctgctcg tcgtgctgcg acg
//
LOCUS       DASUCJ010000440         1479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_249137, whole genome shotgun sequence.
ACCESSION   DASUCJ010000440 DASUCJ010000000
VERSION     DASUCJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249137"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFU90_05270"
     CDS             complement(<1..251)
                     /locus_tag="VFU90_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479220.1"
                     /translation="MRRAFFAIALVLAACGRNERARTPAAPADHPVPQQSAGPDALVL
                     WFPRAGGHVRAFPYPSLDSALWTSADKAPALERVLGFDD"
     gene            complement(314..>1479)
                     /locus_tag="VFU90_05275"
     CDS             complement(314..>1479)
                     /locus_tag="VFU90_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5479221.1"
                     /translation="YRVRFAPDYVARPSVGYATDDFGSGVYGGTAIVLSDMTGNNQLA
                     FAGAINGRIADAQLFAGYTNLARRFQYSTGIYQQPYYFANGYGLAGDAKGFFETQIIT
                     RYMVRQAYGVGIYPLDRFTRVELGMRLTNLERNDLVFTRQLDSSYTQIDGFNEADQND
                     RTVNYVQPLLAYVSDNVLWGYTAPIYGRRYRFQVQPALGSLKWTEYLADYRRYDPILF
                     SYLTVATRVMANISTGRDADSLRKYIGYSDEMRGYDGATFSSAQATCPTTTTSALYRC
                     SPLLGSSVAMASAELRFPIYRGGTIAGVIPLPPIEGAFFYDVGTAWFGGQDVVLHRVR
                     VDDPTNVRGLLTSHGVSIRINMFNYVILGWSYAIPTEATGHRGVWQFSIYPPF"
BASE COUNT          268 a          436 c          541 g          234 t
ORIGIN      
        1 tcatcgtcga atccgagcac gcgctcgagc gccggcgcct tgtccgccga ggtccagagt
       61 gcggagtcga gcgacgggta tgggaatgcg cgaacgtgtc ctccggctcg cggaaaccag
      121 agcacgagcg catcgggacc agcggactgc tggggaacgg gatgatcggc gggcgcggcg
      181 ggagtgcggg cgcgctcgtt ccgaccgcac gccgccagca cgagcgcaat cgcgaaaaac
      241 gctctacgca cgcatgcaag ctaaggcagc gggaaaactt gggaaaggga agcgcgccaa
      301 agcgcgcgct tgtctagaac ggcgggtaga tcgagaactg ccagacgccc cggtgacccg
      361 tcgcttccgt cgggatcgcg tagctccacc cgaggatcac gtagttgaac atgttgatcc
      421 ggatgctcac accgtggctg gtgagcaggc cgcgcacgtt ggtgggatca tcgacgcgca
      481 cgcgatggag caccacgtcc tggccgccga accaggcggt gccgacgtcg tagaagaacg
      541 cgccttcgat gggcgggagc gggatcacgc cggcaatcgt gccgccgcgg taaatcggga
      601 agcgcagctc ggcgctcgcc atggcgacgc tgctgcccag gagcggagag cagcgataca
      661 gcgctgatgt cgtggtcgtg gggcaggtgg cctgcgcgga cgagaacgtc gcgccatcat
      721 agccgcgcat ctcgtccgag tacccgatgt acttgcggag cgagtcggcg tcgcgaccgg
      781 tggagatgtt cgccatgacg cgcgtggcga cggtgaggta gctgaacagg atcggatcgt
      841 accggcgata gtcggccaga tattcggtcc acttgaggct gcccagcgcg ggctgcacct
      901 gaaagcggta gcgccgtccg tagatcggcg cggtgtagcc ccagagcacg ttgtccgaca
      961 cgtaggccag gagcggctgc acgtaattga cggtccggtc gttctggtcg gcttcgttga
     1021 agccgtcgat ctgcgtgtac gacgagtcga gctgccgcgt gaacacgagg tcgttgcgct
     1081 cgaggttcgt taggcgcatg cccaactcga cgcgcgtgaa gcggtcgagc ggatagatgc
     1141 cgacgccgta cgcctggcgg accatgtagc gcgtgatgat ctgcgtctcg aagaatccct
     1201 tcgcgtcgcc ggcgagaccg tagccgttgg cgaagtagta cggctgttga tagatgccgg
     1261 tggagtactg gaagcggcgg gcgaggttcg tgtagccggc gaacagctgg gcgtcggcga
     1321 tgcggccgtt gatcgcgccg gcgaacgcga gctggttgtt gccggtcatg tcgctgagca
     1381 cgatggccgt gccgccgtac acgccgctcc cgaaatcgtc ggtggcgtag ccgaccgagg
     1441 gacgggccac gtaatccggc gcgaagcgca cgcggtagc
//
LOCUS       DASUCJ010000441         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_81076, whole genome shotgun sequence.
ACCESSION   DASUCJ010000441 DASUCJ010000000
VERSION     DASUCJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81076"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFU90_05280"
     CDS             <1..761
                     /locus_tag="VFU90_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU5479222.1"
                     /translation="VIVFVGENESSRAYVRNLVKTGERAGVDVEVDRLGEDASLGAVR
                     ERLERLRDDPAVHGVMLQQPLPPHLPIRAIADAIPANKDVDGTHPMNQGHLAFGSGTD
                     FVPATPAAVMLLLERSPHWPLRGKHAVMVGRSIVVGAPVALLMLAQDASVTVLHKETP
                     DLRPFLRMADVVVVATGVPGLIRGEHLKPGATVIDVGTTVVDGALKGDVDFESVSRVA
                     AAVTPVPGGVGPVTNVALLRNVVKSAERMNSLDV"
     gene            complement(781..1470)
                     /locus_tag="VFU90_05285"
     CDS             complement(781..1470)
                     /locus_tag="VFU90_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5479223.1"
                     /translation="MASMSERLRLFPLNAVLFPGSSLHLHVFEARYKRMIEECLEAGE
                     SFGVALIAEGAEAGDPDVVPHAVGSIAEILDVVPLPQGRYYVTTVGRERFKIVRVLSR
                     DPYLLVEAERIEEREALEDRRLAALRERVRRLFDEYLDIVAELSGRRPEFDLPADARR
                     ASFAVGDALQVDQNVKQRLLELDTAEGRLKVELEVLMQSLPPLRRLLRQRRSSAVDVG
                     TVRFGKYFSRN"
BASE COUNT          282 a          522 c          514 g          261 t
ORIGIN      
        1 tcgtcatcgt tttcgtgggc gagaacgaat cgagccgcgc gtacgtccgg aatttggtaa
       61 aaaccggcga gcgcgccggg gtcgacgtgg aagtcgaccg gcttggggaa gacgcttccc
      121 tcggcgcggt gcgcgaacgg ctcgaacggc tgcgcgacga cccggccgtc cacggggtca
      181 tgctgcagca gcctttgccg ccgcacttgc cgatccgcgc gatcgccgac gcgatcccgg
      241 ccaacaaaga cgtcgacggc acccatccga tgaatcaagg gcatttggcc ttcggcagcg
      301 gcaccgactt cgttcccgct acgcccgcgg cggtaatgct tctgctggaa cgcagcccgc
      361 actggccgct gcgcggcaag catgcggtga tggtcgggcg ctcgatcgtc gtaggcgctc
      421 cggtggcgct gctgatgctc gcgcaagatg cgtcggtaac ggtcctgcac aaggaaacgc
      481 cggatctgcg gccgtttctt cgaatggccg acgtcgtggt ggtcgcaacc ggcgttccgg
      541 gcctgattcg cggcgagcat ctcaagcccg gcgcgacggt gatcgacgtc ggcacgacgg
      601 tcgtcgacgg cgcgctcaag ggcgacgtcg atttcgaaag cgtctcgcgg gtggcggcgg
      661 cggtgacgcc ggtgccgggc ggcgtcggac cggttaccaa cgtggcgcta ttgcgaaacg
      721 tcgtcaagag cgccgagcgc atgaactcac tcgacgtttg aacgtccgcc tcgtcctcaa
      781 ctaatttcgc gaaaagtatt tgccgaagcg caccgtcccg acgtcgaccg cgctgctgcg
      841 ccgttgccgc agcaagcgcc gcagcggcgg taacgactgc atgagaacct cgagttcgac
      901 cttcaaacgg ccctcggccg tatcgagttc gagcagccgc tgcttcacgt tctgatcgac
      961 ctgcagcgca tcgccgacgg cgaacgaagc ccgccgcgcg tcggcgggca agtcgaactc
     1021 cgggcggcgg ccggaaagct ccgcaacgat gtcgagatac tcgtcgaaca ggcgccggac
     1081 gcgttcgcgc agcgccgcga ggcgacggtc ttcgagcgct tcgcgttcct cgatccgctc
     1141 ggcctcgacc agcagatagg gatcgcgcga cagcacccgg acgatcttga aacgctcccg
     1201 cccgacggta gtaacgtagt accttccttg cggcaacggt acgacgtcga ggatttccgc
     1261 aatcgaaccg acggcgtgcg gtacgacgtc cggatcgccg gcttccgccc cttcggcgat
     1321 cagcgcgacg ccgaagcttt caccggcttc cagacactcc tcgatcatcc gtttgtaacg
     1381 cgcttcgaag acgtgaagat gcagcgagct tcccggaaag agcaccgcgt tgagcgggaa
     1441 cagtcgcagg cgctcggaca tagacgccat cagttcgggg aggtcggctg tggttcccgg
     1501 ccaacagaac ctccgaatgg agcacgacgc gcgtttcctc gagacgatcc atccggaacc
     1561 gcacccgctg ctgcgcgaa
//
LOCUS       DASUCJ010000442         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1166210, whole genome shotgun sequence.
ACCESSION   DASUCJ010000442 DASUCJ010000000
VERSION     DASUCJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166210"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFU90_05290"
     CDS             <1..289
                     /locus_tag="VFU90_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479224.1"
                     /translation="HIAEHWALEDGGAIGHKHYQHGGELELEEGRRYIVDVGSVGQPR
                     DLNPAASLAFYEPELRRVQWARYEYPIADVQRKIRAAGLPEYLADRLEVGR"
     gene            286..729
                     /locus_tag="VFU90_05295"
     CDS             286..729
                     /locus_tag="VFU90_05295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5479225.1"
                     /translation="MSEVPFDDGNCFACGPENPVGMHLHFDRAADGDGVFAAVTLAPQ
                     YQGWRGIAHGGIVMALLDEAMAHAAGFAGHRGVTASVNVRFRMPVPLETPLEVRGRVN
                     WQRRNVLGVEATIRDRDGTLLVRGEGNVVSRGALDAAADRRNPSR"
     gene            735..1226
                     /gene="smpB"
                     /locus_tag="VFU90_05300"
     CDS             735..1226
                     /gene="smpB"
                     /locus_tag="VFU90_05300"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5479226.1"
                     /translation="MKSKQQRAAAKVDEKRRDAPAIDNRRARHEYEILESLEAGLALT
                     GTEVKSIRAGGVSLNEAYARFRDGEAWLLGMHVPPYKQGSFSNVDPNRPRKLLMHREQ
                     IVRLHSRVGEKGLTIVPLRLYFTRGIVKVQLGLARGKKLWDKRATAAKRDAERDIARQ
                     VRR"
     gene            1231..2118
                     /gene="tilS"
                     /locus_tag="VFU90_05305"
     CDS             1231..2118
                     /gene="tilS"
                     /locus_tag="VFU90_05305"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5479227.1"
                     /translation="MRGARPERGVERAVEREGVLRRGERVLVACSGGPDSAALGGVLD
                     ALVPGLDLDVRVAHVNHGIRASAGQDECVAAAIAAGLGRPFEAVHLAGAASAEASLRE
                     ARYAALLELAQRRGCTAVATAHHAEDQSETVLLALFRGAGPEGVGGMRVRRPLGDGVD
                     LVRPLLRVPASELRDYCHRRGLPYVVDPSNADLGLRRNAVRSALEALRPAFPGLDEAV
                     ARAAELVAGEREGTGRAQLRRRVRERLAGEDELRDVDFTHVEAAVRTLERGGSGTFHM
                     KAGVRLEILHGTIAGITRE"
     gene            2115..2726
                     /gene="hpt"
                     /locus_tag="VFU90_05310"
     CDS             2115..2726
                     /gene="hpt"
                     /locus_tag="VFU90_05310"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5479228.1"
                     /translation="MTTGGGRAGVPGRRLYSAGEIAAAVKRLAAAIAADWSGKPLVLL
                     GVLKGALYFTVDLSRELAAIPDGPSEIIVEYVCVSSYEGGAESNGEPRILLDPAVPLE
                     GRNILIVDDVADNGFTLDALGAFLKERGAAALGACVLFDKPARRQASVPLDYVGLTLP
                     DAFAVGYGLDYQELYRTLPYLAELDAPGCPPIQGHRKTTVPTS"
     gene            2723..>3098
                     /locus_tag="VFU90_05315"
     CDS             2723..>3098
                     /locus_tag="VFU90_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEU5479229.1"
                     /translation="MSKNLRSIVLIIGAIALVFFLVERFVQPSEGATHLDYGAFYQKL
                     EVGQVQSFHAVGLAATGELTNGTKYNVSVPNVDQTFVDEVYKKVKSGAISFDQQANSG
                     LLSSLIGLAPLAIMVMILFFILR"
BASE COUNT          497 a          990 c         1140 g          471 t
ORIGIN      
        1 gcacatcgcc gaacactggg cgttggaaga cggcggggcg atcgggcaca aacattacca
       61 gcacgggggc gagctggagc tggaggaggg gcgccgttac atcgtcgacg tgggcagcgt
      121 cggacagccg cgcgacctca atcccgcggc gtcgctggcg ttctacgagc ccgagcttcg
      181 ccgagtccag tgggctcgct acgagtatcc gattgccgac gtgcagcgca agattcgcgc
      241 cgccggcctg cccgagtatt tggccgaccg gctcgaggtg ggacgatgag cgaagtaccg
      301 ttcgacgacg gcaactgctt cgcgtgcggc ccggaaaatc cggtggggat gcacttgcat
      361 ttcgaccggg cggccgacgg cgacggggtg ttcgcggcgg taacgctcgc accgcaatac
      421 caaggatggc gcgggatcgc gcacggcggg atcgtgatgg cgctgctcga cgaggcgatg
      481 gcgcacgcgg ccggatttgc cgggcatcgc ggcgtgaccg cgagcgtgaa cgtgcgcttt
      541 cgcatgccgg tgccgttgga aacgccgctg gaagtgcggg gacgcgtgaa ctggcagcgg
      601 cgtaacgtgc tgggcgtcga ggcgacgatc cgcgatcgcg acggcacgct gctggtgcgc
      661 ggcgagggca acgtcgtctc gcggggcgca ttggatgcgg ccgccgatcg ccgcaatccg
      721 agccggtagg acgcgtgaaa tcgaaacagc agcgcgccgc cgctaaagtt gacgagaaac
      781 gccgcgacgc gcccgcgatc gacaaccggc gcgcgcgcca cgagtacgaa attctcgaat
      841 cgctggaagc cgggctcgcg ctcaccggca ccgaggtgaa gtcgatacgc gccggcggcg
      901 taagtttgaa cgaagcctac gcacgctttc gcgacggcga agcgtggctg ctcggcatgc
      961 acgtgccgcc gtacaagcag ggaagttttt cgaacgtcga tcccaatcgc ccgcgcaaac
     1021 tgctgatgca tcgcgagcag atcgttcggc tgcacagccg ggtcggcgaa aagggcttga
     1081 cgatcgttcc gctgcggctg tactttacgc gcggcatcgt gaaggtacag ctcggtctgg
     1141 cgcgcggtaa gaagttgtgg gacaagcgcg cgaccgctgc caagcgcgac gccgagcgcg
     1201 acatcgcgcg gcaggtgcgg cggtgacgcg atgaggggcg cgcgtccgga acgcggcgtc
     1261 gaacgcgcgg tcgagcgcga gggcgtcctg cgccgcggcg aacgggtgct ggtcgcttgc
     1321 agcggcgggc cggattcggc ggcgctgggc ggcgtcctgg acgcgcttgt cccggggctc
     1381 gacctcgacg ttcgggtcgc gcacgtcaac cacgggattc gcgcgtcggc cggtcaggac
     1441 gaatgcgtcg ccgccgcgat cgcggccggg ctcggccggc cgttcgaagc cgtgcacctg
     1501 gccggtgccg cgagcgccga agcgtcgctg cgcgaggcgc gctacgccgc gctgctggag
     1561 ctggcgcagc gccgcgggtg caccgcggtc gcgaccgccc accacgccga ggaccagagc
     1621 gagacggtgc tgctcgcact cttccgcggg gccgggccgg agggggtcgg cgggatgcgc
     1681 gtccgccggc cgctgggcga cggggtggat ctggtccggc cgctgctgcg cgtgccggcg
     1741 agcgagctgc gcgactactg ccaccgccgc ggcttgccgt acgtggtcga tcccagcaac
     1801 gccgacctcg ggcttcggcg caacgccgta cggtcggcgc tggaggcgtt gcggccggcc
     1861 tttccgggac tcgacgaagc ggtcgcccgg gcggccgagc tggtggccgg cgagcgcgag
     1921 ggcaccggtc gcgcgcagtt gcgccgccgg gtccgggagc ggctggccgg cgaggacgaa
     1981 ctgcgcgacg tcgacttcac ccacgtggaa gccgcggtgc ggacgctcga acgcggcggc
     2041 agcggaacct tccatatgaa ggcgggcgtc cggctggaga ttctgcacgg cacgatcgcg
     2101 ggtataaccc gggaatgacg accggcggcg ggcgagccgg agttccgggg cggcggctct
     2161 actccgcggg cgaaatcgct gcggccgtca agcggctggc agccgcgatc gcggccgact
     2221 ggtccgggaa accgctggtg ctgctcgggg tgttgaaagg agcgctgtac tttaccgtcg
     2281 acctctcgcg ggagctcgcg gcgattcccg acggaccgag tgaaatcatc gtggagtatg
     2341 tctgcgtttc ctcatacgag gggggcgccg aatcgaacgg cgagccgcgg atcttgcttg
     2401 acccggcggt gccgctggag ggccggaaca ttctgatcgt cgacgacgtc gccgacaacg
     2461 gattcacgct cgacgcactt ggagcgttct tgaaggagcg tggggcagcc gccctgggag
     2521 cgtgcgtcct gttcgacaag ccggcgcgcc ggcaggcgtc cgtgccgctc gactacgtag
     2581 gcctgaccct gccggacgcc ttcgccgtag gttacggttt ggactaccag gaactgtacc
     2641 gcacgctacc ctacctggcc gagctcgacg cccccggctg cccgccgata caaggtcaca
     2701 gaaagacgac ggttccgact tcgtgagcaa aaatctccgc tcgatcgtac tcatcatcgg
     2761 tgcgatcgct ctggtcttct tcctggtgga gcgattcgtg cagccgagcg agggcgcgac
     2821 ccatctcgac tacggcgcct tctaccagaa gctcgaagtc gggcaagtcc agtcgttcca
     2881 cgcggtcggc ctggcggcga ccggcgagct gaccaacgga accaagtaca acgtttcggt
     2941 accgaacgtc gaccagacgt tcgtggacga ggtctacaag aaggtcaaga gcggcgcgat
     3001 cagcttcgac cagcaggcca actcggggct gctctcgagc ctgatcggtc tcgcgccgct
     3061 ggccatcatg gtgatgattc tgttcttcat tctgcgac
//
LOCUS       DASUCJ010000443         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_325278, whole genome shotgun sequence.
ACCESSION   DASUCJ010000443 DASUCJ010000000
VERSION     DASUCJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325278"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFU90_05320"
     CDS             complement(<1..1159)
                     /locus_tag="VFU90_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5479230.1"
                     /translation="MAVFRADRQTVWDQFRHGGGLNDVVANPNLPARPSWRFTQPRKG
                     TSASPVVAGKLVLVAANDRAVYAVDAADGKPVWQWDGDNEVMSSPVYRDGVAIAGTGD
                     SDSLVWSPPDYAVVGMGRSDLNGIDLRTGKTRWTFALTGTGMPMPALAGDTVLHVDGS
                     GVLVALDARTGAYRWRRLFYSNASMASILLGGNGTAYFGGGFPGAVYAVRSSDGAPVW
                     THRFARGTGSFDDCPLATDGSRIFGMYSAPLEGAPRALVAAGVHARQHAYALDSATGK
                     TRWDTALPVSGVVPAYNESAIPMYAGGTVYYGSAIAPIVTALDARTGRVRWTLHVGGP
                     VKGGFVLRDGALYFGDLGGRLWAVDPVTGRVLGSVQTDLRFNVGSPIALNGS"
     gene            1432..>1955
                     /locus_tag="VFU90_05325"
     CDS             1432..>1955
                     /locus_tag="VFU90_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5479231.1"
                     /translation="MTLLYGDVGAQAFGDEPAIRAVDEIFEAAIRARASDIHIEPCTG
                     GGRVRERVDGTLRESKRVSAHLLPRVLSRIKLLAGMDIADRRLPQDGRYALDRPAAAL
                     EARVSSIPTIAGEKLVIRLLDAHAVVPNLEKLGMSTQCAACFRQALHASAGFVVICGP
                     TGSGKTTTLYAAIT"
BASE COUNT          341 a          661 c          647 g          306 t
ORIGIN      
        1 gcgagccgtt cagcgcgatc ggcgagccga cgttgaaacg caaatccgtt tgtacgcttc
       61 cgagcacgcg gccggtaacg ggatcgaccg cccagaggcg accgccgagg tccccaaaat
      121 acagcgcgcc gtcgcggagt acgaagccgc ccttaaccgg gccgccgacg tgcagcgtcc
      181 agcgaacgcg tccggtgcgg gcgtcgagcg cggtgacgat cggcgcgatc gcgcttccgt
      241 agtagacggt cccgccggcg tacatcggaa tcgcggactc gttgtacgcc ggaaccacgc
      301 cggagacggg cagtgcagta tcccagcgcg tcttccccgt agccgaatcg agcgcgtacg
      361 cgtgttgccg cgcgtggacg cccgcggcta ccagcgctcg cggggcgcct tcgagcgggg
      421 ccgagtacat gccgaagatg cgactgccgt cggtcgcgag cggacagtcg tcgaacgagc
      481 cggtgccgcg cgcgaaacga tgggtccaca ccggggcgcc gtctgaggat cgcaccgcgt
      541 acaccgctcc cgggaagccc ccaccgaaat aggccgtgcc gttaccgcct aggagaatac
      601 tggccatcga cgcgttggag tagaagagcc gccgccagcg gtacgcgccg gttcgcgcgt
      661 cgagtgctac gaggacgccg ctgccgtcga cgtgcagcac ggtgtcgccg gccagcgccg
      721 gcatcggcat gccggttccg gtgagagcga acgtccagcg cgttttcccg gtgcggagat
      781 cgatcccgtt gaggtcgctg cgccccatac cgacgacggc gtaatccggc ggggaccaaa
      841 cgagcgaatc ggaatcgccc gtgccggcga tcgcgacgcc atcgcggtag accggggacg
      901 acatgacctc gttgtcgcca tcccactgcc aaaccggttt tccgtcggca gcgtcgacgg
      961 cgtagacggc gcgatcgttg gcggcgacga gcaccagttt gcccgccacg accggcgagg
     1021 ccgaagttcc cttgcgcggc tgggtgaagc gccagctcgg gcgcgcgggg agattcgggt
     1081 tggcgacgac gtcgttgagc ccgccgccgt ggcggaactg atcccaaacg gtctgccgat
     1141 cggcgcgaaa gactgccaac tcgctttggg cgcgcgctac aaccggcgca acgctcgcaa
     1201 gcgcaaccgc gagcgcgcca agccgcctgc cgtagcccat actctctgcg ccatagcaga
     1261 cccacggccg caaatccttt tcagtacccc gcgcacgtgc gacgaggaca ttaagatcat
     1321 tccggatcga gggctatttc gccccgaccc gtagtgtgct ataaccgttt cctacgcaag
     1381 aaggcgcggc tcgttggccg tactctgttg cgcagacgaa gaggtcgggg tgtgactctt
     1441 ttgtacggcg acgtcggcgc gcaagcgttc ggcgacgaac cggctattcg agccgtcgac
     1501 gaaattttcg aagcggcaat ccgcgcgcgc gcatccgata tccacatcga gccgtgtacg
     1561 ggcggcggtc gcgttcgcga acgggtcgac ggcacgctgc gtgaatccaa acgcgtctcg
     1621 gcacatctgc tgccacgcgt cctatcccga attaagctgc tcgcgggaat ggacatcgcg
     1681 gaccgccggc tcccgcaaga cggccgctac gcgctcgatc gtccggccgc cgccctcgag
     1741 gcgcgcgttt cgtcgattcc gacgatcgcc ggtgagaaac tcgtcattcg ccttctcgac
     1801 gcccacgcgg tcgtcccgaa tttggaaaag ctcggcatgt cgacccagtg tgcggcgtgc
     1861 tttcgacagg cgcttcacgc gagcgccggc ttcgtcgtca tttgcggccc gacgggcagc
     1921 gggaaaacga cgaccctgta cgctgcgatt accga
//
LOCUS       DASUCJ010000444         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_493571, whole genome shotgun sequence.
ACCESSION   DASUCJ010000444 DASUCJ010000000
VERSION     DASUCJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493571"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFU90_05330"
     CDS             complement(<1..652)
                     /locus_tag="VFU90_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479232.1"
                     /translation="MTGSSMSPTVLMADNGPGTVVRIHGRHMGDRVYRGAITFFAVCV
                     PLLVALVAWEIAVAGWPTFRHFGLSFLTSSDWDPVKERFGAAPAIYGTLVSSAIALVI
                     ATPLAVGVSVFLSEFAPRWMRQPVGFLVDLLAAIPSVVYGLWGIFVLLPLLRDPVMPF
                     LRDTLKLGSTPFFTGPAYGPSMLAAGLILAIMVLPYIASVSREVLLAVPRAQREAAL"
     gene            complement(649..>1649)
                     /gene="pstS"
                     /locus_tag="VFU90_05335"
     CDS             complement(649..>1649)
                     /gene="pstS"
                     /locus_tag="VFU90_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5479233.1"
                     /translation="DTTASARSTAGVDLTGAGATFPYPIYSKWFSDYARETSIKINYQ
                     SIGSGGGIRQLTEHTVDFGASDAPMSDDELARANGPIMHFPTVIGAVAVTYNLPSLKG
                     PIRLTGDVLADMFLGHITKWNDSRITADNPGVALPATDVLVVHRAEASGTTYIFSDYL
                     SAVSPDWAKAPGKGKELSWPVGIGAKGNEGVAGQVKQTPGAIAYVELAYAKQNNLPTA
                     QIKNASGAFVAPSIESATAAAAGIADKLPNNTDYRISIVNAPGAGAYPIASFTWLLVY
                     RNQTDATKAKKLKDFLHWYLHSGEATAASLDYAPLPDVMKTRLDARVDSVTVGGAA"
BASE COUNT          291 a          540 c          546 g          272 t
ORIGIN      
        1 cgagggcagc ctcccgttgg gcgcgcggca ccgcgagcag cacttcgcgc gacaccgacg
       61 cgatgtacgg gagcaccatg atcgcgagaa tgagtcccgc cgccagcatg ctcggtccgt
      121 atgcggggcc ggtgaagaac ggcgtgctgc ccagtttgag cgtgtcgcga aggaacggca
      181 tcaccggatc gcggagcaac ggcagcagca cgaagatgcc ccagagaccg tacaccacgc
      241 tcggaatcgc ggccaggaga tccaccagga atcctacggg ctggcgcatc caccgcggcg
      301 cgaactcgga gaggaacacc gacacgccca ccgccagcgg cgtcgcgatg actaacgcaa
      361 tcgcggacga gaccagcgtg ccgtagatcg ccggcgcggc gccgaagcgc tccttcaccg
      421 gatcccagtc gctcgacgtg aggaacgaga gcccgaagtg ccggaacgtc ggccagccgg
      481 ctactgcgat ctcccacgcc accagcgcca ccagcagcgg gacgcacacc gcgaagaacg
      541 tgatggctcc gcggtagact cgatctccca tgtgccgccc gtggatacgg acgacggtcc
      601 ctggtccgtt gtcggccatg agcacggtcg gagacatcga ggagccggtc atgccgcgcc
      661 gcccaccgtc acggagtcca cacgcgcgtc gagtcgcgtc ttcatcacgt cggggagagg
      721 cgcgtagtcg agcgatgccg ccgtcgcttc gccggagtgc agataccagt ggaggaagtc
      781 cttcaacttt ttcgccttgg tcgcgtcggt ttgattgcga tacacgagca gccacgtgaa
      841 cgacgcgatg gggtaggcac ccgcgccggg cgcgttcacg atcgaaatcc gatagtcggt
      901 attgttaggc agcttgtcgg cgatgcccgc cgcggcggct gttgccgact cgatcgacgg
      961 cgccacgaac gcacccgatg cgttcttgat ctgcgccgtg ggcaggttgt tctgcttcgc
     1021 gtacgcgagc tccacgtagg cgatggcgcc gggtgtctgc ttcacctggc cggccacgcc
     1081 ttcgttgccc ttcgcgccga tgccgacggg ccacgacagc tcctttcctt tgccgggcgc
     1141 cttggcccaa tcggggctca ccgccgacag atagtcggag aagatgtagg tcgtaccgct
     1201 cgcctcggcg cgatgcacga ccagcacatc ggtggccggc agcgcgacgc cgggattgtc
     1261 cgctgtgatg cggctgtcgt tccacttcgt gatgtggccg aggaacatgt cggcgagcac
     1321 atcgccagtt aggcgaatcg gacctttgag cgacggcaag ttgtacgtga ccgcgacggc
     1381 cccgatcacc gttgggaaat gcatgatcgg cccgttggct ctcgcgagct cgtcgtcgga
     1441 catcggcgca tccgatgcac cgaaatcgac cgtgtgctcg gtgagctgcc ggatgccccc
     1501 gcccgacccg atcgactgat agttgatctt gatgctcgtc tcgcgcgcgt agtccgaaaa
     1561 ccacttgctg tagatcggat acgggaaggt cgcgccggcg ccggtgaggt cgacacccgc
     1621 ggtggatctg gccgatgcgg tagtgtcgc
//
LOCUS       DASUCJ010000445         1323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_799735, whole genome shotgun sequence.
ACCESSION   DASUCJ010000445 DASUCJ010000000
VERSION     DASUCJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799735"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFU90_05340"
     CDS             complement(<1..746)
                     /locus_tag="VFU90_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479234.1"
                     /translation="MGQTHDYDVVIVGAGPAGLTAGLYAGRAMLRTAVLERGAAGGEL
                     LNTELIEDYPGFERIEGWELAQRLEEHTRKFGAELALGVTVDRVHRAADGTFETTTDA
                     GDVYRSPAVIVTAGGTPVKLGVPGEAEYAGKGVSYCAICDGAFFKGHTIAVVGGGDAA
                     AEESDFLTRYAEKVYLIHRRHELRASKIIQQRVFENPKIEIIWDTVVEIVEGDTHGLV
                     DNLVLRNVHTNETRDLAVTGLFVFVGFRPNT"
     gene            complement(834..>1323)
                     /locus_tag="VFU90_05345"
     CDS             complement(834..>1323)
                     /locus_tag="VFU90_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479235.1"
                     /translation="FGSLDILVNSAAIMVRRSLDELTPDDWDDTFALNLRAPFFCARA
                     AARAMADRGGVIVNLADLAGIETWPAYAAHGISKAGVIHMTRVLARILAPRVRVNAIA
                     PGAVLLPESWSADDAAHLERTTPLRRLGSPNDVAQAMLYFIEADYVTGDLLIVDGGRH
                     IR"
BASE COUNT          225 a          468 c          408 g          222 t
ORIGIN      
        1 gtattcggac gaaaccccac gaacacgaac aggcctgtca ccgccagatc gcgcgtctcg
       61 ttcgtgtgca cgttgcgcaa caccaggttg tcgaccaggc cgtgcgtgtc gccctcgacg
      121 atctccacca cggtgtccca gatgatttcg attttcgggt tctcgaacac gcgctgctgg
      181 atgatcttgg acgcgcgcag ctcgtgacgc cggtggatga gatagacttt ctcggcgtac
      241 cgcgtgagga aatcgctttc ctccgcggcg gcgtcgccgc ctccgacgac ggcgatcgtg
      301 tggcccttga agaacgcgcc gtcacagata gcgcagtacg acacaccctt cccggcgtac
      361 tcggcttccc cgggcacgcc taacttgacc ggcgtgccgc ccgcggtgac gatcaccgcc
      421 ggcgaccgat agacgtcgcc ggcatcggtg gtggtctcga aggtgccgtc ggccgcccga
      481 tgcacccggt ccaccgtcac gcctaacgca agctcggccc cgaatttccg ggtgtgctcc
      541 tcgagcctct gcgccagctc ccagccctcg atgcgctcga acccgggata gtcttcgatg
      601 agctccgtat tgagcagctc gccgccggcc gcgccgcgct ccaggaccgc cgtgcgcagc
      661 atcgcccgtc ccgcgtacaa ccccgccgtc agacccgccg gtccggcccc aacgatcacc
      721 acgtcgtagt cgtgtgtctg acccaccgcc atgctccttc gatcacgggt tgagggaccg
      781 ccgttggtcc tctgcactcc gaaagataaa gcgtcgggaa gatcaccccg ttcctagcga
      841 atgtgccgcc cgccatcgac gatgagcaga tcaccggtca cgtagtcggc ctcgatgaag
      901 tacagcatcg cctgcgcgac atcgttcgga gaacccagac gacgcaacgg tgtggtccgc
      961 tcgagatgcg ccgcatcgtc ggcgctccag ctctcgggta gcaacacggc gcccggcgcg
     1021 atcgcgttca cgcgcacgcg cggtgcgagg atccgcgcga gcacgcgcgt catgtgaatg
     1081 acgccggcct tcgagatgcc gtgcgctgcg tacgcgggcc aggtttcgat ccccgcgaga
     1141 tcggcgagat tcacgatcac gccgccgcga tccgccatcg cgcgcgctgc cgcacgggca
     1201 cagaaaaacg gtgcgcgcag attgagcgcg aacgtgtcgt cccagtcgtc gggtgtgagc
     1261 tcgtcgagcg agcgccgcac catgatcgcg gccgagttga cgaggatgtc gagcgagccg
     1321 aac
//
LOCUS       DASUCJ010000446         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_921708, whole genome shotgun sequence.
ACCESSION   DASUCJ010000446 DASUCJ010000000
VERSION     DASUCJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921708"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..>1201
                     /locus_tag="VFU90_05350"
     CDS             80..>1201
                     /locus_tag="VFU90_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5479236.1"
                     /translation="MPSPAKPQEGATNGAEPKQITYDPAKAFGAIARIYRQSDAMSPQ
                     QQTRLQNLVDRYCAKTKRSKEHTQEHRKHHADPRVVNGFKPRIKELIYPIVTSRASGC
                     RLWDLDGNEYVDALNGFGSNFFGYSAKVIADALHAQIDRGFEIGPQTTLAGESAALVC
                     ELTGMDRAAFCNTGSEAVMGALRIARTVTGRSLVVAFSGSYHGIFDEVIVRDTKTMRS
                     VPAAPGILQESVANILVLEYGTEKSLAVIRERASEIAAVLVEPVQSRRPDFRPKAFLQ
                     EVRKITKASGSAMIFDEVITGFRAHPAGAQAFYEIEADLATYGKVVGGGMPIGVIAGR
                     NPWMDALDGGQWQYGDDSAPTAGVTYFAGTFVRHPMAMAA"
BASE COUNT          207 a          431 c          383 g          180 t
ORIGIN      
        1 ccccacgctc gtttccgcag ccgctcccgc ttccccgccc gccccagttc aggtcgcgcc
       61 ggcttctgtc gagcccgcag tgccctctcc cgcgaaacct caggagggcg cgaccaacgg
      121 agcggagccg aagcagatca cgtacgaccc cgcgaaggcg ttcggcgcca tcgcccgcat
      181 ctaccgccag agcgacgcga tgtcgccgca gcagcaaaca cgcctgcaga acctcgtcga
      241 tcgctactgc gccaagacga agcgctccaa ggagcacacg caagagcacc gcaaacacca
      301 cgccgatccg cgcgtcgtga acgggttcaa gccgcgcatc aaggagctca tctatccgat
      361 cgtgacatcg cgcgcgtccg gctgccgtct ctgggacctc gacggcaacg agtacgtcga
      421 cgcgctgaac ggcttcgggt cgaacttctt cggctactcg gccaaagtca tcgccgacgc
      481 gctgcacgca cagatcgacc gcggcttcga gatcggtccg cagacgaccc tcgcgggcga
      541 gtccgcggcc ctcgtttgcg agctgaccgg catggaccgg gcggcgttct gcaacaccgg
      601 ctccgaagcg gtcatgggcg cgctgcgcat cgcgcgcacc gtcacggggc gatcgctcgt
      661 cgtcgccttc tcgggctcgt accacggcat cttcgacgaa gtgattgtcc gggacacgaa
      721 gacgatgcga tcggtcccgg cggctccggg catcctgcaa gagtcggtgg cgaacatcct
      781 cgtgctcgaa tacggcacgg agaaatcgct cgccgtgatt cgcgagcgcg cgtccgagat
      841 cgctgcggtt ctcgtcgaac cagtccagag ccggcgcccc gatttccggc cgaaggcgtt
      901 cctccaggag gtgcggaaga tcaccaaggc ctccggcagc gcgatgatct tcgacgaggt
      961 catcaccggg ttccgcgccc accccgcggg cgctcaggcg ttctacgaaa tcgaggcgga
     1021 cctcgcgacg tacggcaaag tcgtcggcgg cgggatgccc atcggcgtca tcgcggggcg
     1081 caatccgtgg atggacgcgc tcgacggcgg ccagtggcag tacggggacg actccgcgcc
     1141 gaccgcgggc gtcacgtact tcgccggtac gttcgtccgc catccgatgg cgatggccgc
     1201 g
//
LOCUS       DASUCJ010000447         1452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_264665, whole genome shotgun sequence.
ACCESSION   DASUCJ010000447 DASUCJ010000000
VERSION     DASUCJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264665"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..942)
                     /gene="whiA"
                     /locus_tag="VFU90_05355"
     CDS             complement(73..942)
                     /gene="whiA"
                     /locus_tag="VFU90_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU5479237.1"
                     /translation="MISADTKDALARDVPASVHCREALLWGLALYGAAGKAEFVTHRN
                     AIARLFWSLLSDRKSHPIETRPPTRLQHLPTFAIALPGELRTDPPKAARKCDRLMEIR
                     AAFLACGSLAAGAHGYHLEFVLASSPLAGRLEWTLRSAGVPPKLTARKGRTVAYYKDF
                     DAIVELLTLIGAFGAVLRLEDVRALRETKNRIHRLVNTEAANLERTAAAAAAQRHVIE
                     YLRSAYGLPQLTPALREIAELRLAHPDESLAELGRRCNPQIAKPTVSSRLTALSRLAE
                     RVRRGQGLPKQAR"
     gene            complement(939..1214)
                     /locus_tag="VFU90_05360"
     CDS             complement(939..1214)
                     /locus_tag="VFU90_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU5479238.1"
                     /translation="MRLDKFMKVSRLSKRRSEAHEALEHGRITKDGKALKPGYQVKPG
                     DVLEIHYATRYVTVAVREVPLRVTPGIKPAELYEILETRKDEPADWI"
BASE COUNT          223 a          455 c          509 g          265 t
ORIGIN      
        1 cccgatgcgg ccgaagccgt tgattccgat acgcatgaac ggcgctccct tgaaggaaag
       61 tgtaggcctg tcttaccggg cctgcttcgg cagcccctgc ccccgccgca cgcgctcggc
      121 cagacgcgag agcgcggtga ggcggctgct gacggtcggt ttggcgatct gcggattgca
      181 gcggcgaccg agctcggcga gcgactcgtc cggatgcgcg aggcgcaatt cggcgatctc
      241 ccgcagtgcg ggcgtcagct gcggcaagcc gtaggcgctg cgcaggtatt cgatcacgtg
      301 gcgttgagcg gcggccgcgg cggcggtgcg ctcgaggttc gccgcctcgg tattcaccag
      361 ccggtggatg cggtttttcg tctctcgcag cgcgcgtacg tcttccaggc gaaggaccgc
      421 gccgaaggcc ccaatcagcg tgagcagctc gacgattgcg tcgaagtcct tgtagtacgc
      481 gaccgtgcgc ccttttcgcg cggtgagttt cggcggtacg cccgcgctgc gcagcgtcca
      541 ctccaggcgc ccggcgagcg gcgacgacgc gagcacgaac tcgagatgat agccgtgcgc
      601 gccggcggcc agcgatccgc aggcgaggaa agccgcgcga atctccatca aacggtcgca
      661 cttgcgagcc gctttcggcg gatcggtgcg cagttcgccc ggcaaggcga tcgcaaacgt
      721 cggcaggtgc tgcagtcggg tcggcggccg cgtttcgatg gggtgcgact tgcggtcgct
      781 cagcagcgac cagaacagtc gcgcgatcgc gtttcgatgg gtaacgaact cggctttccc
      841 ggcggcgccg tacaacgcca ggccccagag caacgcttcg cggcaatgaa cgctcgccgg
      901 gacgtcgcgc gcgagcgcgt cttttgtgtc ggccgagatc atatccagtc ggccggttcg
      961 tctttacgcg tctcgagaat ttcgtagagc tcggccggct tgattccggg ggtgacgcgc
     1021 agcggaacct cgcgcacggc gacggtgacg tagcgcgtcg cgtagtgaat ctcgagaacg
     1081 tcccccggtt tgacttgata gcccggcttg agcgccttcc cgtctttggt gatgcggccg
     1141 tgctcgagcg cttcgtgcgc ttcgctgcgc cgctttgaga gacgggagac cttcatgaac
     1201 ttgtcaagcc gcacgcggga cgggttcgtg ctcgctccga tcggctccgg gcgtcgcgcg
     1261 gcgctcgaac ggcgccaagg gcaccaactg aagagtattg acagtatgcc gatatatcga
     1321 atactgtagc gacatgagag gacaattctg gggcggcccc ggttgggcgg ccttcgcagc
     1381 gggcggcttc ggacggcggg tgcggcgttt caaacgcggc tttctgaagt tcgtcgtgct
     1441 caagctgctg gc
//
LOCUS       DASUCJ010000448         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_814916, whole genome shotgun sequence.
ACCESSION   DASUCJ010000448 DASUCJ010000000
VERSION     DASUCJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814916"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFU90_05365"
     CDS             complement(<1..799)
                     /locus_tag="VFU90_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479239.1"
                     /translation="MSVDVLQAEKLSGEGESKAALALLNRHLAQHPNDARALVDRGDV
                     YEALDDQRAAIADYSAAIAVNPVYAYAYASRCESRWEIDQNHEALADCDKAVELDPRM
                     GYALRQRALVELDLGDLEAARRDADRAVELSPDQPYSLSTRCRVRVASGDYDGAVKDC
                     NASIGLDDTRTTPFFYRGRAEIAQKNWGAAIADFSNVLDKDPTDFGAYYWRALARQNT
                     AAYASGLKDVDRYIAAHPDDGDGHLLRAQLELGLGNRSEARAAATEAL"
BASE COUNT          166 a          341 c          323 g          186 t
ORIGIN      
        1 gcaaagcctc ggtcgccgcc gcccgcgctt cgctgcgatt gcccaaccct agttcgagct
       61 gagcgcgcag caggtgtccg tcgccgtcgt cggggtgcgc cgcgatgtag cgatcgacgt
      121 ccttgagtcc gctcgcgtag gccgcggtat tctgtcgagc cagcgcgcgc cagtagtacg
      181 ctccgaaatc ggtgggatct ttgtcgagca cgtttgaaaa atcggcaatc gcggcgcccc
      241 agtttttctg agcgatctcg gcacgtccgc gatagaaaaa cggcgtcgtg cgcgtgtcgt
      301 cgaggccgat ggaggcattg caatctttga cggcgccgtc gtaatcgccg cttgccacgc
      361 gcaccctaca ccgggtcgac aagctatacg gctggtcggg gctcagttcc accgcgcggt
      421 cggcgtcgcg acgcgccgcc tcgaggtcgc ccaggtccaa ctcgaccaag gcacgctggc
      481 gcaaagcgta acccatacgc gggtcgagct cgacggcctt atcgcagtcg gcgagcgcct
      541 cgtgattctg atcgatttcc caacgcgatt cgcaccgcga cgcgtaggcg tacgcataga
      601 ccggatttac cgcgatcgcg gctgagtagt cggcgatcgc cgcgcgctga tcgtcgagcg
      661 cttcgtagac gtcgccgcga tccaccagcg cccgcgcgtc gttgggatgc tgcgccaagt
      721 gccggttgag gagtgccagc gcggccttgg actctccctc cccgctcagc ttttcggcct
      781 gcaggacgtc gacgctcacg ctcgtctcgg gctcgggcgc cgcactgcct cgggcggcaa
      841 acccggcaca aagtacgatc gccgctgcgg cgctgccggc gcgacggaaa atcgttgcaa
      901 ccatagtttg ccctccgaat gcggcggtcg gcgacgcaca aaagcccgtc gcgactacga
      961 cgggcttttg tttagagcta aatctggcac cgacctactt tcgagggacc ctgcgg
//
LOCUS       DASUCJ010000449         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_325364, whole genome shotgun sequence.
ACCESSION   DASUCJ010000449 DASUCJ010000000
VERSION     DASUCJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325364"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="prfA"
                     /locus_tag="VFU90_05370"
     CDS             complement(<1..1011)
                     /gene="prfA"
                     /locus_tag="VFU90_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5479240.1"
                     /translation="MTSIQARLETMLRRFDEIEAELANPGGSFDQARYTALLKERARL
                     EAPVATFRRLRKTRDEIASSEALARESDGEMRALAEDEARALRERERELQDELQGLMI
                     PRDPNDDKDVFVEIRAGAGGDEAGIFAGDLARAYMRFAESLGMTVELASENAGDAGGY
                     REIVFGVRGGDGPYRYFKHESGVHRVQRVPETEAQGRIHTSTATVAVLPQVEDDVEVE
                     IKPGDLQIDTYKASGAGGQYVNKTESAIRITHVPTGIIVASQQERSQTQNREKAMQML
                     RAMLYDRKRREQEEAVGSLRRSQVGTGDRSEKIRTYNFPQDRVTDHRINRNFGNMRGI
                     MNG"
     gene            complement(1079..1351)
                     /gene="rpmE"
                     /locus_tag="VFU90_05375"
     CDS             complement(1079..1351)
                     /gene="rpmE"
                     /locus_tag="VFU90_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5479241.1"
                     /translation="MKEKIHPKWHPQARVHCACGNTFTTGSTLPEIAVEICAACHPLF
                     TGQQKLIDTAGRVDKFNQRAAAAKKKQEEAAARSATRAARKAAAEA"
     gene            1452..2093
                     /locus_tag="VFU90_05380"
     CDS             1452..2093
                     /locus_tag="VFU90_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479242.1"
                     /translation="MKPASPSVWLGAAVFAVALLAGGTRFAVPTSAATPPPTPPPLPP
                     PATPAADAATPGPTSTPIPIPSGFGIPAMTGSPAPGKATPSPPPDARKGIEGVWEVQI
                     QRGTSTDYAHFRLEQTGTTLTGSFLNDAGKRFPLAGSLDGQSVRLVVSMPDGTSLLLE
                     GRLDGTTDMIGMITTPQEQVPFTAAYRPKEKWIDNLNASPGGLGGTGGGYSPP"
     gene            complement(2107..2616)
                     /locus_tag="VFU90_05385"
     CDS             complement(2107..2616)
                     /locus_tag="VFU90_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5479243.1"
                     /translation="MESKLRGHAHKYGKNVDTDVIIPGKYCNIVDQAELGKHALEGLD
                     PEYAGRMQAGDIIVADRNFGCGSSREVAPIAIKGSGTAAVIAKSFARIFYRNALNIGL
                     PIFESEEAVDGIESGDEIEVEPASGVVRNLTKGTEYRAAEFPPFMRALIDAGGLVPYV
                     EKRLAEAGS"
     gene            complement(2621..>3364)
                     /locus_tag="VFU90_05390"
     CDS             complement(2621..>3364)
                     /locus_tag="VFU90_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5479244.1"
                     /translation="LRTVGELGIDGATYRAIEYHGSTVDELSITGRITMANMAIEAGA
                     KNGIFHADAKTIAYVTERTNRPFTVESADADAVYERVLEIDVDALEPQVACPHTPDNV
                     HPISEVAADDIAVDQVFVGSCTNGYIEDLRIVAKILEGKRIANSVRVIVNPGSQKVWM
                     QAAQEGILTTLAAAGCAVNTPGCGACFGGHMGTLGDGERAVSTTNRNYVGRMGSPKAE
                     VYLASPATCAASALTGKITDPRAVVLAEV"
BASE COUNT          474 a         1157 c         1071 g          662 t
ORIGIN      
        1 gccgttcatg atgccccgca tgttcccgaa gttgcggttg atccggtgat cggtcacgcg
       61 atcctgcggg aaattatagg ttcggatctt ctccgagcgg tcgccggtgc ctacttggga
      121 acgccgcagc gaaccgaccg cttcttcctg ctcgcggcgt ttgcgatcgt acagcatcgc
      181 gcgcagcatc tgcatggctt tctcgcggtt ttgggtttgc gaacgctcct gctgcgaggc
      241 cacgatgatt ccggtcggga cgtgcgtgat gcgaatcgcc gactcggtct tattgacgta
      301 ctggccgccc gccccggacg ccttgtaggt gtcgatctgc aaatcgcccg gtttgatctc
      361 gacctcgacg tcgtcctcga cctgcggcaa gacggccacc gtggcggtgc tcgtgtggat
      421 gcggccttgc gcttcggtct ccggtactcg ctgcacgcga tgcaccccgg actcgtgctt
      481 gaagtagcga tacggtccgt ccccgccgcg cacgccgaac acgatctcgc gatatccgcc
      541 ggcgtcgccg gcgttttccg acgcgagctc caccgtcatc cccagcgact ccgcgaaccg
      601 catgtacgcg cgcgcgaggt cgccggcaaa gattcccgcc tcgtcgccgc ccgcgccggc
      661 gcgtatctcg acgaatacgt ccttgtcgtc gttgggatcg cgcgggatca tcagtccctg
      721 cagctcgtcc tgcagctcgc gctcgcgttc gcgcagggcg cgcgcctcgt cttcggcgag
      781 cgcccgcatc tcgccgtcgc tctcgcgcgc gagcgcttcg ctggaagcga tctcgtcgcg
      841 cgtcttgcgc agccggcgga acgtcgcgac cggcgcttcc agccgcgccc gctctttcag
      901 cagcgcggta tagcgtgcct gatcgaagct tccgccgggg ttggccagct cggcttcgat
      961 ttcgtcgaat cgccgcagca tcgtttcgag ccgcgcttga atggacgtca ctctacctcc
     1021 gtcaaaacaa aagacgagcc gcattccgag cggctcgtct tcgaacgttc cggcgcgctt
     1081 aagcttcggc ggcggctttg cgcgcggcgc gcgtcgcgct gcgagcggcg gcctcttcct
     1141 gcttcttctt ggcggcggcg gcgcgctgat tgaacttgtc gacccggccg gcggtatcga
     1201 tgagcttctg ctgccccgtg aacagcggat ggcaggcggc gcaaatttct acggcgattt
     1261 ccggcagcgt cgaaccggtc gtaaacgtgt ttccgcaggc gcagtgcacg cgcgcttgcg
     1321 ggtgccactt cgggtggatc ttctctttca cagcttctgc attatagcag aggcggcaag
     1381 agggttgccc tgtcggccgt atgagcgctc ggacgggagg tgcggccgct tttccgacct
     1441 aagctggccg cgtgaaaccc gcatcgccgt ccgtctggct gggcgccgcc gtcttcgccg
     1501 ttgcgctgct tgcgggcggg acgcgcttcg ccgtgccgac ctcggccgcc accccgccgc
     1561 ccaccccgcc gccgctcccg ccgccggcta cgccggccgc cgacgcggcc acgccggggc
     1621 cgacctcgac gcccatcccg atcccatccg gattcggaat tcccgcgatg acgggcagcc
     1681 ccgcacccgg caaggcgacg ccgagcccgc cgcccgacgc gcgcaaaggg atcgaagggg
     1741 tgtgggaagt gcaaatccag cgcggtacct cgacggatta cgcgcacttc aggctcgagc
     1801 agaccggcac gacgctgacg ggcagcttcc tcaacgacgc cggcaaacgg tttccgctgg
     1861 cgggctcgct cgacggccaa tcggttcgcc tcgtcgtctc gatgcccgac ggaacgtcgc
     1921 tgctgctcga gggacgtctc gacggcacta ccgacatgat cggaatgatc acgacgccgc
     1981 aggagcaggt tccatttacc gccgcctacc gccccaaaga gaagtggatc gacaacctga
     2041 acgcctctcc cggcgggctc ggcggaaccg gcggaggcta ctcgccgccc tagcggcgga
     2101 cgctcgctag gagccggctt cggccagccg cttctccacg tacggtacga ggccgccggc
     2161 atcgatcagc gcccgcatga acggcgggaa ctcggccgcc cggtactccg tgcctttggt
     2221 gaggttgcgc accacgccgc tcgccggctc gacttcgatc tcgtcgccgc tttcgatccc
     2281 gtcgaccgct tcttccgact cgaaaatcgg cagcccgatg ttcagcgcgt tgcgataaaa
     2341 aattcgggca aagctcttgg cgattacggc ggcggttccc gagcctttga tggcgatcgg
     2401 cgccacttca cggctggagc cgcagccgaa gttccggtcg gcgacgatga tgtcgccggc
     2461 ttgcatgcgc ccggcgtatt cggggtcgag cccttccagc gcgtgcttcc ccagctccgc
     2521 ttgatcgacg atgttgcagt acttgccggg aatgatcacg tcggtatcga cgttctttcc
     2581 gtatttgtgc gcgtgcccgc gcagtttcga ctccatcgcg ttatacctcg gccagcacga
     2641 cggcgcgcgg atcggtgatc tttccggtga gcgcggaagc ggcgcacgtc gccggtgaag
     2701 cgaggtacac ctccgccttc ggcgagccca tccggcctac gtagttccgg ttggtggtcg
     2761 aaacggcgcg ttctccgtcg ccgagcgttc ccatgtgccc gccgaagcaa gctccgcacc
     2821 cgggcgtgtt taccgcgcag ccggccgccg cgagcgtcgt gaggatgccc tcttgggcgg
     2881 cctgcatcca gactttctgg ctgcccgggt tgacgatcac gcgcacgctg tttgcgatcc
     2941 gcttgccttc gagtattttc gccacgatcc gcagatcttc gatatatccg ttggtgcacg
     3001 agccgacgaa cacttggtcg accgcgatgt cgtcggctgc gacttccgaa atcgggtgga
     3061 cgttgtcggg cgtgtgcggg caggcgactt gcggttcgag cgcgtcgacg tcgatttcga
     3121 ggacgcgctc gtagacggcg tccgcgtcgg cgctttcgac cgtgaacggc cggttggtac
     3181 gttccgtaac gtacgcgatc gtcttcgcgt cggcgtggaa gatgccgttc ttcgctcccg
     3241 cttcgatggc catgtttgcc atcgtgatgc ggcccgttat cgagagttcg tctaccgtcg
     3301 agccgtggta ctcgatcgcg cggtaggtcg cgccgtcgat tccgagctcg cccaccgtgc
     3361 gcag
//
LOCUS       DASUCJ010000450         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_447996, whole genome shotgun sequence.
ACCESSION   DASUCJ010000450 DASUCJ010000000
VERSION     DASUCJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447996"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFU90_05395"
     CDS             <1..204
                     /locus_tag="VFU90_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479245.1"
                     /translation="GRHHRNRRNVVRPDYERNEKERDQAGRQQRVEIFADGRRRGRPP
                     RDGCKWFGRERDARDGEKAEEAE"
     gene            complement(226..576)
                     /gene="rplS"
                     /locus_tag="VFU90_05400"
     CDS             complement(226..576)
                     /gene="rplS"
                     /locus_tag="VFU90_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5479246.1"
                     /translation="MNVIDALNRDQLKDAVPAFRAGDTVKVYSKVVEGGKERVQMFEG
                     VVTVRKGGGAKASITVRRVAHGVGVEKTFLVHSPRVERIEVGKRGVVRRSRLYYLSDK
                     IGKAARIKEKKVVK"
     gene            complement(653..>1114)
                     /locus_tag="VFU90_05405"
     CDS             complement(653..>1114)
                     /locus_tag="VFU90_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479247.1"
                     /translation="RRAIAVPSPSGTPFCQADAARFAALDRVIFVCGHYEGIDDRLAS
                     LYSVEEYSLGDFVVTGGEIPALAMMDATVRLLAGALRPESLESESFSGSLLDYPSFTR
                     PPVFRGVAVPEVLLSGDHARIAEWRREQSRRRTAIRRRDLLDDALHPEERP"
BASE COUNT          202 a          340 c          385 g          187 t
ORIGIN      
        1 ggtcggcacc atcgaaaccg acggaatgta gtacgtccgg attacgaacg taatgagaaa
       61 gagcgcgatc aggccggccg ccagcagcgc gtcgagatat tcgcggacgg ccgccgccga
      121 ggccgacctc cccgggacgg ctgcaaatgg tttggtcgag agcgcgacgc gcgcgacggc
      181 gaaaaagcag aggaggccga gtagctgcag cggtgtcagg gccggctact tgacgacttt
      241 cttctctttg atgcgggcgg cttttccgat cttgtcgctg aggtagtaga gccggctccg
      301 gcggacgacg ccgcgcttgc cgacttcgat ccgctcgacg cgcggactgt gcacgaggaa
      361 cgtcttttcg actccgaccc catgagcgac gcggcgtacg gtgatcgacg ctttggcacc
      421 gccgcccttg cgaacggtca ccacgccttc gaacatctga acgcgttcct tgccgccctc
      481 gacgactttg gaatagacct ttacggtgtc gcccgcgcgg aacgcgggga cggcgtcttt
      541 gagctgatcc cgattgaggg catcgatgac gttcatgaca cttttctgct ggcggtgaaa
      601 aaaaatgcgc cgggcgtagg tgagccgggc tttggacaac cacggtatgg tatcacgggc
      661 gttcctccgg gtgcaaggcg tcgtccagca ggtcccggcg ccggatcgcg gtccgccgcc
      721 gggattgctc gcggcgccac tcggcgatcc tggcatggtc gccggagaga aggacttcgg
      781 ggacggccac gccgcggaag acgggcggcc gcgtgaagct cggatagtcg agcaggctgc
      841 cggaaaacga ctcgctttcg agggattcgg ggcgcaaggc gccggccagc aggcggacgg
      901 tcgcatccat catcgcgagc gccggaatct cgcccccggt cacgacgaag tcgcccagac
      961 tgtactcttc gaccgaatag agcgaagcta gccggtcgtc gatcccttcg tagtggccgc
     1021 agacgaagat cacgcggtcg agggctgcga accgggcggc gtcggcttgg caaaaaggtg
     1081 tgccgctcgg gctcggtacg gcgatcgcac ggcg
//
LOCUS       DASUCJ010000451         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1013462, whole genome shotgun sequence.
ACCESSION   DASUCJ010000451 DASUCJ010000000
VERSION     DASUCJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013462"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>1011
                     /locus_tag="VFU90_05410"
     CDS             124..>1011
                     /locus_tag="VFU90_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479248.1"
                     /translation="MAARLPLGLDLGSSRVRLARAERSAEGPQIAAVATRDLPDDAVS
                     AYTVNDVELVAAIVEDLRHEVGGRERRCVLSVNVPTASLRLVHFPDMGASERRRAAAF
                     EAERLTEQEDGDAASVVRVHPVPGGNGLHAVGIVRRTTLAARTLCAKRSGLLPIAVDH
                     DVLAYRRSFPRHDAVLDVGLHCATLHAFAPGGLQSTSIPSAGLDITRGIASDLSIDAN
                     AAEKRKRTLGTAGAGEGSRNSFVFRVKAAIEKARERAPLRRIALVGNGARLIGFAAVL
                     ESAVGATAETAVSELLRGSA"
BASE COUNT          159 a          328 c          346 g          178 t
ORIGIN      
        1 tagcggagga gcgttcgcgc tagccctgat cctagcgcgc aaggcgcgtc gcgacacgag
       61 ggttagtttt gctcccttct tggcactcgg agcctcgtgc atcacgctgg tatggcacta
      121 acgatggccg cacggttgcc gctgggcctc gacttgggat cgtcgcgcgt tcggcttgcg
      181 cgtgcggagc gctcggccga gggcccgcaa atcgcggcgg tcgcgacccg tgaccttccg
      241 gacgacgccg taagtgcgta taccgtcaac gacgtcgagc tggtggcagc catcgtcgag
      301 gatttgcgac acgaagtagg cggccgagaa cggcggtgcg tcctgtcggt gaacgttccg
      361 acggcatcgc tgcgactcgt tcactttccg gatatgggcg cttccgagcg acgtcgcgca
      421 gccgcctttg aagccgaacg cctaaccgag caggaagacg gcgatgccgc ctcggtcgtg
      481 cgcgtgcatc cggttcccgg cggcaacggc ctccacgccg tcggtatcgt gcgacggacg
      541 acgctggcgg cccgaaccct atgcgcgaaa cgttccgggt tgctcccgat cgcggtggat
      601 cacgacgtcc tcgcgtaccg gcgttcgttc ccccggcacg acgccgtcct cgacgtcgga
      661 ctgcactgcg cgacgttgca cgcctttgca ccgggaggac tgcaatcgac gtccataccg
      721 agtgccgggc tcgatatcac ccggggaatc gcttcggatc tttcgatcga cgcgaacgcc
      781 gccgagaagc gcaagcgcac cttgggcacc gccggcgccg gcgaaggatc gcgaaacagc
      841 ttcgtgtttc gcgtaaaggc ggcgatcgag aaggcccgcg agcgggcacc gctgcgacgc
      901 attgcgttgg tcggaaacgg tgcccgtctg atcggttttg cggcggtact cgagtcggcg
      961 gtcggggcta ctgcggagac ggcggtcagc gaacttctcc gcggcagcgc t
//
LOCUS       DASUCJ010000452         1999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_952279, whole genome shotgun sequence.
ACCESSION   DASUCJ010000452 DASUCJ010000000
VERSION     DASUCJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952279"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFU90_05415"
     CDS             <1..908
                     /locus_tag="VFU90_05415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU5479249.1"
                     /translation="NQVQAQITDSALRAIINDYTREAGVRNLEREIGTVFRKVARKIA
                     EAPKLRARVKSENLSEYLSKPRFFNEVRKRVASVGVATGLAYTPVGGDILFIETQVMP
                     GTGKLVLTGQLGDVMKESAQAAVSFLRSRSGELGLSEDYFAKHDVHVHVPAGATPKDG
                     PSAGVALATSIASILTGLKVDPNLAMTGEITLTGQVLPIGGLKEKVLGAKRAGIGKIL
                     FPKRNEVDLDDVPKEVRDTMTFVPVEELSEVLYHALGKRVISPVPLGAEGAVKSNVVP
                     MRRRNGVAKRARANDRKRRAIARKR"
     gene            complement(920..1957)
                     /gene="selD"
                     /locus_tag="VFU90_05420"
     CDS             complement(920..1957)
                     /gene="selD"
                     /locus_tag="VFU90_05420"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU5479250.1"
                     /translation="MNAAGIRLTELSSCAGCAAKLGAAELHAVLRRVRAADPDPRVLV
                     GYGTSDDAGVYLVSDGVALVQTVDFFTPIVDDPFDFGRIAATNALSDVYAMGGTPLTA
                     LNIVGFPEDLDGAILSQILDGGAAVAAEAGVAVLGGHTIKDAEPKYGMAVTGIVDPAR
                     IVTNAAARPGDALVLTKPLGTGILTTALRRGAIAAEELGEAIRWMTARNDVAARTMLA
                     HGVRAATDVTGFGLLGHAGGMASASGVAIAVDAAAVPLMPRVLELIAGGVVPGGTLHN
                     ARTHAEFTTFAPRVGDALRIALSDAQTSGGLLIAVAPERADALSRDLRAAGVPAVRIG
                     AVRPGSGIAVS"
BASE COUNT          350 a          665 c          688 g          296 t
ORIGIN      
        1 tgaaccaggt acaggcgcaa atcaccgatt cggcattgcg cgcgatcatc aacgattaca
       61 cccgcgaggc gggggtgcgc aacctggaac gcgagatcgg aaccgtgttc cgcaaggtcg
      121 cccgcaagat cgccgaggcg ccgaagctgc gggcgcgggt gaagtccgag aacctttcgg
      181 agtacctcag caagccgcgc ttcttcaacg aagtgcgcaa gcgggtggcg tcggtcggcg
      241 tggctaccgg cctcgcctat acgccggtcg gcggcgacat tctgttcatc gaaacccagg
      301 tgatgcccgg aaccggcaag ctcgtgctca ccgggcagct cggcgacgtg atgaaagaaa
      361 gcgcccaggc ggcggtgtcg ttcttgcgat cgcgttccgg cgaactgggg ctttcggaag
      421 actactttgc caagcacgac gtgcacgtgc acgtgccggc gggtgccacg ccgaaagacg
      481 gcccgtcggc cggcgtcgcg ctcgcgacct cgatcgcgtc gattctcacc ggcctgaaag
      541 tcgatccgaa cctcgcgatg accggcgaaa tcacgcttac cggtcaggtt ctgccgatcg
      601 gcggactaaa agaaaaagtg ctcggcgcca aacgcgccgg catcggcaag atactctttc
      661 ccaagcgcaa cgaggtcgat ctcgacgacg tccccaagga ggtccgcgac acgatgacct
      721 tcgtgccggt cgaagagctg tcggaagtgc tctaccacgc gctcgggaag cgcgtcatct
      781 cgccggtgcc gctcggcgcc gagggagcgg tgaagagcaa cgtcgtcccg atgcgacgcc
      841 gcaacggcgt cgccaaacgg gcgcgggcga acgatcgcaa gcggcgcgcg atcgcacgca
      901 agcgctgatt ctcgcgatcc tacgaaacgg cgatccccga gccgggacga accgcgccga
      961 tccggacggc cggcacgccc gccgcgcgca aatctcgcga tagggcgtcg gcccgctccg
     1021 gagcgacggc gatcagcagc ccgcccgacg tctgcgcgtc ggagagtgcg atccgcaacg
     1081 cgtcgccgac tcgaggtgcg aacgtcgtaa attcggcgtg cgtccgggcg ttgtgcagcg
     1141 taccgccggg gacgacgccg ccggcgatca gctccaaaac gcgcggcatc aacgggaccg
     1201 cggcggcgtc gacggcgatc gcgacgccgg atgcgctcgc catcccgccg gcgtgtccga
     1261 gcagaccgaa accggtgacg tcggttgcgg cgcgcacgcc gtgcgcgagc atcgttcgcg
     1321 cggcgacgtc gttgcgcgcg gtcatccagc ggatggcttc gccgagttct tcagccgcga
     1381 ttgcgccgcg gcgcagtgcg gtcgtgagga taccggtgcc gagcggtttc gtgaggacga
     1441 gcgcgtcgcc gggacgcgcc gcggcgttgg tgacgatgcg cgccggatcg acgatgcccg
     1501 tgaccgccat accgtatttc ggctcggcgt ccttgatcgt gtgtccgccg agcaccgcga
     1561 cgcccgcctc ggcggcgacc gccgcgccgc cgtccagaat ttgcgagagg atcgcgccgt
     1621 cgagatcttc cgggaaccca acgatgttga gcgccgtgag cggcgtgccg cccatcgcgt
     1681 agacgtcgga gagcgcgttg gttgcggcga tgcggccgaa gtcgaacggg tcgtcgacga
     1741 tcggcgtaaa gaaatcgacc gtctgtacca gcgcgacgcc gtcgctcacg agatagacgc
     1801 ccgcgtcgtc gctcgttccg taaccgacca agacgcgcgg gtccggatcg gcggcccgga
     1861 cgcggcgcag caccgcgtga agctcggcgg cgccgagctt tgcggcgcaa cccgcgcacg
     1921 agctgagttc ggtgaggcgg atgcctgcgg cgttcataga agctctattc catgaaaaag
     1981 acgctgctgc tccttcccg
//
LOCUS       DASUCJ010000453         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_539546, whole genome shotgun sequence.
ACCESSION   DASUCJ010000453 DASUCJ010000000
VERSION     DASUCJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539546"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2520
                     /locus_tag="VFU90_05425"
     CDS             88..2520
                     /locus_tag="VFU90_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5479251.1"
                     /translation="MHALLLAACCLIGGNVHAPSGAPIAHAQVVLSGTQSARTSTDVR
                     GNFAFTVPPGHYTIDAVAGGYASVQAYAGDVTADTRIEIVLEPSDTPKLRTIGEVSVN
                     GGYSLVRNVIPETNVSRAQMDAQGYSSVLEALKQVPSLVIQYPDSGSPTAPAVVSLRG
                     PDPSEALVTLDGQTLNDGNTGDLDLSQFAVPAFGGVNVTEGLGPTDSEGSNTFGGAVN
                     LVSLRPTLEPHFSLSGSLGSYGTSQSWLNATGTIGKLGYALAGNVYQQAGQVDQYALV
                     YPSNNLPAKNCGPNAPPGATPQNCPLLTHLGSTIDARLGMVNLDYNFSQRADAGVRVF
                     TLGNFRDESSAVNGIAGNPFEPCDPTDAQAPPCPSGFTSTPNPNLGEHVGSGNSIFAQ
                     NVRAYDAYSRSVLGSGSLLADYYAGDNNVDVSGGSGGTSPYDVSHRDKRYNEGLSWGR
                     TFETSEFAFGGYARQESLTGAGITSTLSQSIDSYFVRGTQQVGSRLRLSGGLYDAQYS
                     TFGNTFNWRLGASYDAGEASVVRFSAGTGFRAPLLIEQYYFPPVLVNGKPEPNPGLPP
                     PDQNCVVAGQGNPDERPEHATEYELGFSHLFSSDSLLDVSLYRSNLRDTIENYYPFGA
                     CSSKLGYAYEIPINIGNAVYEGAELRFKQRFPRQNLAMTLSYGLNVAYPYSLGPNVSN
                     PTSGGSLVDDEQFLGVPQQQGSALVEWAQNGWHAATSFTFVGRNNPLNQAPYTLTDLA
                     VGKTLGRLDFTVAATNIFNSVSGPFTYYNAGTPYRGLYAGPGGSSYLANLPTDRFSVL
                     PASVRFILTLHQ"
     gene            2733..3500
                     /locus_tag="VFU90_05430"
     CDS             2733..3500
                     /locus_tag="VFU90_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479252.1"
                     /translation="MKPYTFAIFAAALTAAGGCARASGNAQAAATPGIVWQAGSPTLG
                     RWVAANLGQCGTPVQNGREFRFTLVQAGTGCGRNQANPLDRDGGIFLLAPGKTYTWTW
                     HETDGPPPGMGRDAQAESLVWQIHGLTEPHTPCARLGFINGPDQVSAPQMWGFFTCSG
                     SSTESTLVWSGAYVPGESDDWKITARISNGTDGWVELWRNGVKQGRWTGATFHNAEQC
                     WWNFGPYKWRWLLAGEGGSTMTTVSQTFQGMTLTLEP"
     gene            complement(3507..>5364)
                     /locus_tag="VFU90_05435"
     CDS             complement(3507..>5364)
                     /locus_tag="VFU90_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479253.1"
                     /translation="GFAAGDLEESSREPGQLGAAALQAFVTFVCERLSLAVVAVCSVV
                     MVLVFVTVEVNATRIGYYPLHFENNTDRVQVQSGSSAGGLQTGDRIDLQALTPQQRFS
                     LLAPPSDAQMTVLVTRGQRTFPVHVTASPPDYSRRAMLARDIGIPLSFFLSLGLASAL
                     FLMRPRPITLAFYVYTMLMLVKVYQTPLDLATWPMNVASDLAIQVVYPAAQLMILVFA
                     QRLYGRPSRTFPLFFGSALALSVLVFVAWVDPIVWTVFQRFGLPGPTRLMMSVSDTLL
                     LVVVLCGLAYIASGATAIDRRRVSWVVAGIALSPILDLTWALTDIISALVGDRSTVLL
                     AISVWTDALAPWFGLLGVIFVLYGFLSQRVVDLRVVIGRAVLYGATTLVLVLIFGVVE
                     WWAEQLFESTRPALYLSLAAALVIGFAMKAIHGRIEDFLNRVFFREQRRAEEVLGRAA
                     RALANTSSQKTVVEFLITEPVAVLGLTSAALFLSAEGDGAFERVASEGWTGGETERID
                     AEDPLIVALRADLSSIQLDGRQLVGIAALPGGRKTPSLIVPLVMRGSVFGFVFYGSRS
                     DGTGLTPSERSLLEAIALSAAAAYDHIDADRSRARIRALEERLSELGAAASG"
BASE COUNT         1003 a         1798 c         1721 g          842 t
ORIGIN      
        1 tcgctcacgg tgagcccgcc gctttcggcg caacccgaaa ccatcctatc gaggcacgcc
       61 gtacggatgc gtcgaagcgg gcggcggatg cacgccctcc tgctcgcggc atgctgtttg
      121 atcggcggaa acgttcacgc accgtcgggt gcgccgatcg cgcacgcgca agtcgttctc
      181 tccggtacgc agagcgcacg aacgtcgacc gacgttcgcg gcaattttgc atttacggta
      241 ccgcccgggc actacacgat cgacgcggtc gccggcgggt acgcttcggt gcaagcctac
      301 gccggcgacg taaccgccga cacgcgcatc gagatcgtgc tcgaaccttc cgatacgccc
      361 aagctgcgca cgatcggcga agtcagcgtg aacggcggct attccttggt gcgcaacgtg
      421 atccccgaaa cgaacgtttc gcgcgcgcag atggatgcgc aagggtattc gagcgtgctc
      481 gaagcgctca aacaggtacc gtcgctggtc attcagtacc cggattccgg ttcgcctacc
      541 gcgccggcgg tcgtctcgct gcgcgggccc gatccatcgg aggcactggt tacgctcgac
      601 gggcagacgc tcaacgacgg caacaccggc gatctcgatc tctcgcagtt cgcagttccc
      661 gcgttcggcg gcgtcaacgt taccgaaggg ctgggcccca ccgatagcga ggggagcaac
      721 acgttcggcg gcgcggtgaa tctcgtgtcg ctgcggccga cgctggaacc gcatttcagc
      781 ctttccggat cgctcggctc ttacggcacg tcgcagtcgt ggctcaacgc gaccggtacg
      841 atcggaaagc tcggctacgc gctggccggc aacgtctatc agcaggccgg acaagtcgat
      901 cagtacgcgc tggtgtatcc gtcgaacaac ctgccggcga agaactgcgg accgaacgca
      961 ccgccgggcg caacgccgca aaactgcccg ctgctcaccc atctcggttc gacgatcgac
     1021 gcacgcctcg gcatggtcaa tctcgactac aacttttcgc agcgggccga cgccggcgtg
     1081 cgcgtcttca cccttgggaa cttccgcgac gagagcagcg ccgtcaacgg catcgccggt
     1141 aacccgttcg aaccgtgcga ccccaccgac gcgcaagccc cgccttgtcc gagcggcttc
     1201 acgtcgacgc cgaaccccaa cctcggcgag cacgtcggca gcggcaactc gattttcgcg
     1261 caaaacgtac gcgcctacga cgcctattcg cgctccgtgc tcggttcggg atcgctgctg
     1321 gccgactact atgccggcga caacaacgtc gacgtctccg gcggctcggg cggaacctcg
     1381 ccgtacgacg tctctcatcg ggacaaacgt tacaacgaag gtctctcgtg ggggcggacg
     1441 ttcgagacga gcgagtttgc gttcggaggg tacgcgcgcc aggagtcgct taccggcgcc
     1501 ggcattacca gcacgctgtc gcagagcatc gattcgtatt tcgttcgcgg cacgcagcag
     1561 gtcggcagcc gcctgcgcct ttcgggcgga ctctacgacg cgcagtactc gaccttcggc
     1621 aacacgttca actggcggct cggcgcgagt tacgacgcgg gggaggcgag cgtcgtgcgc
     1681 ttctcggccg gcacgggctt tcgcgcgccg ctgctgatcg agcagtacta tttcccgccg
     1741 gtattggtca acgggaagcc ggaacccaat ccgggccttc cgccgccgga ccaaaactgc
     1801 gtggtcgcgg gtcaagggaa tcccgacgaa cggccggagc acgccaccga gtacgagctg
     1861 ggattctcgc acctgttttc gagcgactcc ctgctcgacg tctcgctgta tcgctcgaat
     1921 ctgcgcgaca cgatcgagaa ctactatccg ttcggtgcgt gcagcagcaa gctcggctac
     1981 gcctacgaga tcccgatcaa catcggtaac gcggtgtacg aaggcgccga gctgcgcttc
     2041 aagcagcgct tcccgcgcca gaatctcgcg atgaccctct cctacgggct caacgtcgcc
     2101 tatccgtatt cgctcggccc gaacgtttcg aatccgacct cgggcggcag tctagtggac
     2161 gacgaacagt tcctcggcgt gccgcagcaa cagggttcgg cgctggtgga atgggcgcag
     2221 aacgggtggc acgccgccac ctcgttcacg ttcgtggggc gcaacaatcc gctcaatcag
     2281 gcgccgtaca cgctcaccga cctggccgtc ggcaagaccc tcggacgact cgactttacg
     2341 gtcgccgcca ccaacatttt caactcggtg tccggccctt ttacgtacta taacgccggc
     2401 actccgtatc gcgggctgta cgcgggtccg ggcggatcgt cgtatctcgc caacctcccc
     2461 accgatcgct tcagcgtatt gccggcgtcg gtgcgattca ttctgacgct tcatcagtag
     2521 cgcccggcgg agggcgcaag gtttgcacgc cgccgcagtt cgggcagcgt ccgccggttg
     2581 cgtccgagca ctccggacaa aacgtgcagc cgtacgagca cacgaaggcg cgggcctccg
     2641 gccccagcgc gacgccgcag ccttcacatt cggacttcat ggattgcgag gttgcccgtg
     2701 agggccgtgc gaatccttga aaagcggtcg acatgaaacc ttacacgttt gcaatattcg
     2761 cagccgcact gacggcggcc ggcgggtgcg cgcgggcatc cggcaacgcg caggcggcgg
     2821 cgactccggg gatcgtttgg caagccggct cgccaacgct cgggcgatgg gtcgccgcca
     2881 acttaggtca gtgcggaacc cccgtgcaaa acggtcggga gtttcgcttt acgctcgttc
     2941 aggcgggaac cgggtgcggc cgcaatcagg ccaatccgct cgaccgcgac gggggcattt
     3001 tcttgctcgc tccggggaag acgtacacct ggacgtggca cgagaccgac ggcccgccgc
     3061 cggggatggg ccgcgacgcg caagcggagt cgctcgtctg gcaaattcac ggcttgacgg
     3121 agccgcacac gccctgcgcg cggctgggat tcatcaacgg tccggatcaa gtgagcgcgc
     3181 ctcaaatgtg gggattcttc acctgcagcg gcagcagcac ggaatcgacc ctcgtttggt
     3241 cgggcgcgta cgtgccgggc gaaagcgacg attggaaaat tacggcacgc atctccaacg
     3301 gcaccgacgg ttgggtcgag ctgtggcgca acggcgtcaa gcaggggcgc tggacgggcg
     3361 cgacgttcca caacgccgag caatgctggt ggaacttcgg gccgtacaag tggcgctggc
     3421 tgctcgcggg cgaaggcggc tcgacgatga cgaccgtttc gcaaaccttt caaggcatga
     3481 cgcttacgtt ggaaccatag cgtaggctaa ccgcttgcgg ccgcgccgag ttccgacaag
     3541 cgctcttcca gcgcgcgaat gcgggcgcgc gaacggtcgg catcgatgtg gtcgtaggcg
     3601 gccgccgcac tcagcgcgat cgcttcgagc agcgagcgct ccgacggcgt aaggccggtg
     3661 ccgtcgctgc ggctgccgta aaaaacgaac ccgaacacgc tgccgcgcat gaccagcggg
     3721 acgatcaggg acggcgtttt gcggccgccc ggcagcgccg cgattccgac cagctgccgt
     3781 ccgtcgagtt gaatcgacga gaggtccgcg cgcagcgcga cgatcagcgg atcctcggcg
     3841 tcgatccgtt cggtctcgcc gccggtccag ccctcggagg cgacgcgctc gaacgctccg
     3901 tcgccctccg cggagagaaa gagtgcggcc gacgttagcc cgagcacggc aaccggctcg
     3961 gtgatcagaa actcgacgac ggttttctgc gacgaggtgt tggcgagcgc gcgggcggcg
     4021 cggcccagca cctcttcggc ccggcgctgc tcgcgaaaga acacgcggtt gaggaaatct
     4081 tcgattcgcc cgtggatcgc tttcatcgcg aaaccgatga cgagcgccgc cgcgagactg
     4141 agatacagcg ccggccgggt gctttcgaag agctgctcgg cccaccactc gaccacgccg
     4201 aagatcagca ccagcacgag cgtggtggcg ccgtagagaa ccgcgcgccc gatgacgacg
     4261 cgcaaatcga cgacccgctg cgacaagaat ccgtacagca cgaagatcac gccgagcaat
     4321 ccgaaccacg gcgcgagcgc gtcggtccac acgctgatcg ccagcaacac ggtcgagcga
     4381 tcgccgacca gcgcactgat gatgtcagta agcgcccagg tgagatcgag gatcggcgag
     4441 agcgcgatgc cggcgaccac ccagctcacg cgccggcggt cgatcgcggt cgcgcccgac
     4501 gcgatgtacg cgagcccgca caacaccacc accagcagca gcgtgtcgga gacgctcatc
     4561 atgagacgcg tcggccccgg caggccgaac cgctggaaaa cggtccagac gatcgggtcg
     4621 acccaagcga cgaacaccag caccgacagc gccagcgcgc tgccgaagaa cagtggaaag
     4681 gtgcggctcg gccggccgta gagccgctgc gcgaaaacga gaatcatcag ctgggccgcc
     4741 ggatagacga cttgaatcgc gagatcgctg gcgacgttca tcggccacgt cgccaaatcg
     4801 agcggcgtct gatagacctt gacgagcatc agcatcgtgt agacgtagaa tgccagcgtg
     4861 atcgggcgcg ggcgcatcag gaacagcgcc gacgccagcc cgagcgacag aaaaaagctc
     4921 agcgggatgc cgatgtcgcg cgccagcatc gcccggcgcg aatagtccgg cggactcgcg
     4981 gtgacgtgca ccgggaacgt gcgttgtccg cgcgtcacca atacggtcat ctgggcatcc
     5041 gacggcggag ccaaaagcga aaaccgctgc tgcggcgtga gcgcctggag atcgatgcgg
     5101 tcgccggttt gcagcccgcc ggcggacgag ccggactgca cctgcacccg gtcggtgttg
     5161 ttctcgaagt gcagcgggta gtagccgatc cgggtcgcgt tgacctcgac cgtcacgaag
     5221 accagcacca tcacgacgct gcagaccgcc accacggcga gcgagagccg ctcgcaaacg
     5281 aaggttacga acgcctgcaa cgccgccgct cccagctggc cgggctcacg cgagctttct
     5341 tctaagtccc cggcggcaaa gcct
//
LOCUS       DASUCJ010000454         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_203641, whole genome shotgun sequence.
ACCESSION   DASUCJ010000454 DASUCJ010000000
VERSION     DASUCJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203641"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFU90_05440"
     CDS             complement(<1..286)
                     /locus_tag="VFU90_05440"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5479254.1"
                     /translation="MNSVFEVIAEPNRRAILSLLVSSEQSVGDIERRLRMPQPTVSKH
                     LRVLREAGFVESTVDAQRRLYRLKPEPLQEVDDWLAQFRRFWSTHIDALER"
     gene            complement(365..>1147)
                     /locus_tag="VFU90_05445"
     CDS             complement(365..>1147)
                     /locus_tag="VFU90_05445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5479255.1"
                     /translation="GNDRPMHSADGGDVRALLTNVSARSSSRAELWTSFLAATGLARV
                     AEVGVWKGDFAAALLAGCPAIDTYLMIDPWRHLDDWNKPANVATDTFETIRQEAMAKT
                     DFAASRRRVMRGTTLEVVDTIDDGSLDFAYIDGDHTLRGIITDLIAMRPKIRAGGFLG
                     GDDFCPSVWQHGPAYEPTLVCPAAVHVALAMRATIFALPFNQFLIAMPNGERGAGGFV
                     DLVGRYGALDLASQVRPPHAGARARLRGALAALRRRAGRPRG"
BASE COUNT          181 a          368 c          405 g          193 t
ORIGIN      
        1 ggcgctcgag cgcatcgatg tgggtggacc agaagcgccg gaactgcgcg agccagtcgt
       61 cgacctcttg cagtggttcc ggcttgagtc ggtacagacg gcgctgtgcg tccacggtgg
      121 actccacgaa cccggcctcg cgcagcacgc gcaggtgctt ggagacggtg ggttgcggca
      181 tgcgaagccg gcgctcgatg tctcccacgg actgttccga cgagaccagg agactcagaa
      241 tcgcgcgccg gttcggctcg gcgatgactt cgaacacgga gttcatggat tgaatatact
      301 ggtggtggta tatacgtgtc aaggtatatg ccgaacgcat cgccccgcgt ccgcgggcgc
      361 tgcgctatcc cctgggccgg ccggcacgcc gccggagcgc cgccagggcg cctcggagcc
      421 gagcgcgggc gcctgcgtgc ggaggccgca cctgcgatgc gaggtccagc gcgccgtagc
      481 ggccgacgag gtcgacgaac ccgccggccc cgcgctctcc gttcggcatc gcgatcagaa
      541 attgattgaa cggcagtgcg aagatggtcg cccgcatcgc gagcgccacg tgcacggccg
      601 cggggcacac gagggtcggt tcgtatgccg gtccgtgctg ccacacgctc ggacagaagt
      661 cgtcgccgcc caggaatccg ccggcgcgaa tcttcggtcg catggcgatg agatcggtga
      721 tgatcccgcg cagcgtgtgg tcgccatcga tgtacgcgaa atcgagcgag ccgtcgtcga
      781 tggtatcgac gacttcgagc gtcgtcccgc gcatcacccg tcggcgcgat gcggcgaaat
      841 cggtcttcgc catcgcttcc tgccggatgg tttcgaacgt gtcggtggcg acgttggcgg
      901 gtttgttcca gtcgtcgaga tgccgccacg gatcgatcat caggtacgtg tcgatcgcgg
      961 ggcatccggc gaggagggcc gcggcgaagt cgcctttcca tacgccgact tcggcgacgc
     1021 gcgcgagtcc ggtcgcggcc agaaacgacg tccagagctc cgcgcgagag ctcgatcggg
     1081 cgctcacgtt cgtcaggagc gcgcgaacgt cgccgccgtc ggcgctgtgc atcggtcgat
     1141 cgttgcc
//
LOCUS       DASUCJ010000455         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1120471, whole genome shotgun sequence.
ACCESSION   DASUCJ010000455 DASUCJ010000000
VERSION     DASUCJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120471"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFU90_05450"
     CDS             complement(<1..187)
                     /locus_tag="VFU90_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479256.1"
                     /translation="MRRSNFLLGTISGLAVVANGDHVIARALAQSPLPGLPGGTGRCL
                     VLVNLNGGNDGLNCVVPH"
     gene            complement(195..1682)
                     /locus_tag="VFU90_05455"
     CDS             complement(195..1682)
                     /locus_tag="VFU90_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEU5479257.1"
                     /translation="MALPQTPVDFGGMSRPQGRLDAATALAPYAGPLTQRSAAHLLRR
                     AGFGGTPDEVRRYAAMPVGRAVESLVRFPASANAQAAPGDVYSPVELLSQYGPQGVRA
                     LSPEQRKALNREIRGSEFRSTLALQLWWLNRMLATPAPLQEKMALYFHGHFTSTAVQK
                     GISPAMIYNQNQLFRDNALGNLRELTRKVSTDPAMLVYLDNDANFKLHPNENYARELM
                     ELFTLGVDQYSEDDVRNSARAWTGWRFDRFTETAAFDPRFHDDGLKTFLGRTGNFTGD
                     DVVNIIFAQPQCARFFAAFLLNSFVYNDPEPELVEGVAQLLRKHDYELAPVMSALLRS
                     NVFYSERAYRALVKSPVEFVVGSYRTLGLPQMDASVLPVLRQMGQVLFYPPNVAGWPG
                     GANWLTSDTMIARQNFLKRLLNSQTIAASSWLRDVPMHPRDATQTLIAAILQGDVAPA
                     SSFEIAGYLGGSGTAALAALTPENYDQRVSGAAYLAMAMPAYQLN"
     gene            1806..1946
                     /locus_tag="VFU90_05460"
     CDS             1806..1946
                     /locus_tag="VFU90_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479258.1"
                     /translation="MALLETHLDGASEAFKKNAARMEGLVDELRRRLIAVREGGGADT
                     VA"
     gene            1959..3389
                     /locus_tag="VFU90_05465"
                     /pseudo
     CDS             1959..3389
                     /locus_tag="VFU90_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
BASE COUNT          635 a         1121 c         1167 g          554 t
ORIGIN      
        1 cgtgcgggac gacgcagttg aggccgtcgt tgccgccgtt gagattcacg agcaccaggc
       61 agcggcccgt gccgcccggc agcccgggca gcggcgactg ggcgagcgcg cgcgcgatca
      121 cgtgatcgcc gttggcgacc accgcgagtc ccgaaatcgt tccgagtaaa aagttgcttc
      181 gtctcattac atcgtcaatt cagctggtag gcgggcatcg ccatcgcgag gtaggcggcg
      241 ccgctgacgc gctggtcgta gttttccggg gtcagcgcgg ccagcgccgc ggtgcccgag
      301 ccgccgagat agccggcgat ctcgaacgac gaggccggcg cgacgtcgcc ctgcaggatc
      361 gcggctatca gcgtctgggt ggcgtcgcgg ggatgcatag gaacgtcgcg cagccacgac
      421 gatgcggcga tcgtctgcga gttcagcaaa cgcttcaaga agttctgccg cgcgatcatc
      481 gtgtcgctgg tgagccagtt cgcgccgccc ggccagccgg cgacgttggg cggatagaac
      541 agcacctgcc ccatctggcg cagcaccggc aagaccgagg cgtccatctg cgggagcccg
      601 agcgtgcggt acgacccgac gacgaactcg accggcgatt tcacgagcgc gcggtaggca
      661 cgctcggagt agaagacgtt gctgcgcagc agcgccgaca tcaccggcgc cagctcgtaa
      721 tcgtgcttgc gcagcagttg cgcgacgccc tcgaccagct ccggttcggg atcgttgtag
      781 acgaacgaat tgagcaggaa agccgcaaaa aagcgcgcgc actgcggttg ggcaaagatg
      841 atattgacga cgtcgtcgcc ggtgaagttg ccggtgcgcc cgaggaacgt cttgaggccg
      901 tcgtcgtgaa agcgcggatc gaacgcggcg gtctcggtga accggtcgaa gcgccagccc
      961 gtccaggcgc gcgccgagtt gcggacgtcg tcttcggaat actggtcgac gccgagcgtg
     1021 aacagttcca tcagctcgcg cgcgtagttc tcgttgggat gcagcttgaa gttcgcgtcg
     1081 ttgtcgagat agaccagcat cgcggggtcg gtcgacacct tgcgggttag ctcgcgcaga
     1141 ttgccgagcg cgttgtcgcg gaacagctga ttctggttgt agatcatcgc cggcgaaatg
     1201 cccttttgaa ccgccgtgct ggtgaaatgg ccgtggaagt acagggccat cttctcttgc
     1261 agcggcgcgg gcgtcgccag catccggttt agccaccaca gctgcaaggc gagcgtcgag
     1321 cgaaactcgc tgccgcggat ctcgcgattg agcgctttgc gctgctcggg gcttagcgcg
     1381 cgcaccccct gcggaccgta ctgcgacagt aactcaaccg ggctgtagac gtcgccgggt
     1441 gctgcttgcg cgttcgccga cgccggaaac cgcacgagcg actcgaccgc gcgcccgacc
     1501 ggcatcgcgg cgtagcgccg tacctcgtcg ggcgtgccgc cgaagccggc gcgccgcagc
     1561 agatgcgcgg cggagcgctg ggtcaacgga ccggcgtacg gtgcgagtgc ggttgcggcg
     1621 tcgagacgtc cctgcggacg gctcatgccg ccgaaatcga ccggcgtctg gggtaaagcc
     1681 atcggccgta agtatagcat tccgggcctg tgacgccgcc ccgaagcccg cacatctaac
     1741 ccgcgcttca tggccggaga cctgcctacc ggaacgagcc ggccaagcgc cgaaacgcgc
     1801 ggccgatggc actcttggag acgcacctcg acggcgcttc ggaggcgttc aagaaaaacg
     1861 ccgcgcgcat ggaaggcctg gtcgacgaac tgcgccggcg gttgatcgcc gtgcgcgaag
     1921 gcggcggtgc cgataccgtc gcgtaacacc gcaagcgcaa caagctaacc gcgcgcgaac
     1981 ggatcgaacg gctggtcgac cccggctcgg attttctcga actatcggcg ctggccgccg
     2041 gcgggatgta ctccgacgac tcgccgtcgg ccggcatcgt gaccggcatc ggcgtcgtgg
     2101 aaggacagca ctgcgcgatc gtcgccaacg acgcaaccgt caagggcggc acgtactatc
     2161 cgatgacggt caagaagcat ctccgcttgc aggagatcgc cgagcaaaac catcttccgt
     2221 gcatctatct ggtcgactcg ggaggtgcgt tcttaccgct gcaggccgac gtcttccccg
     2281 accgggatca cttcgggcgg attttctaca atcaggcgcg gatgtcgagc aaacgcattc
     2341 cgcaaatcgc cgccgtcatg ggatcgtgca cggccggcgg tgcgtacgtg ccggcgatga
     2401 gcgacgaaac ggtaatcgtc aaaggcaacg gcacgatctt tctgggtgga ccgccgttgg
     2461 tcaaagcggc caccggcgaa gaggttaccg ccgaagagct gggcggcgcc gacgtgcata
     2521 cgcgcatctc gggcgttgcc gaccacttcg ccaacgacga cgcgcacgcg ctcgcgatcg
     2581 tccgcgaaat cgtcgcgaat ctccatctcg agctgccgcg ccagtgggac cgcgcgcaac
     2641 cccagccgcc gaaatacgat ccgcgcgaca tctacggcgt gatcccggcc gacagccgcg
     2701 tgggttacga cgttcgcgag gtaatcgcgc ggctggtcga cgcctcggag ctgcacgagt
     2761 tcaaggcacg ctacggcacg acgctcatct gcggattcgc tcatatcgaa ggccatccga
     2821 tcggcatcct cgcaaacaac ggcattttat tcagcgagag cgcgctcaaa ggcgcgcact
     2881 tcatcgagct gtgcgcgcag cggggaacgc cgctgctctt cctgcagaac atcaccggct
     2941 tcatggtcgg caaggagtac gaaaacggcg ggatcgccaa ggacggcgcg aagctcgtga
     3001 ccgccgtcgc gtgcgccgaa gtaccgaagt tcacggtggt gatcggcggc agtttcggcg
     3061 ccggcaacta cggcatgtgc ggacgcgcgt attcgccgcg ccagctttgg atgtggccga
     3121 acgcgcgcat cagcgtgatg ggcgggccgc aagccgcgag cgtgctttcg accgtcaagg
     3181 gcgcgctctc gccggaagaa aaaacggcct tcgaggctcc gatcctcgag aaatacgagc
     3241 gcgaaggaaa tccctactac tcgaccgcgc gtctgtggga cgacggaatc atcgacccgc
     3301 tggacacgcg ccgcgcgatc gcgatcggcc tcgatgccgc cgcgcacgcg ccgcagccgc
     3361 gcacgtcgtt cggcatcttc cgcatgtagg ctagcccggc ggaaagcgcc cgggaaacag
     3421 cgcgtaatac ttcggcgttt cggtccaggc gccgttgcga tagctcagca cggcggc
//
LOCUS       DASUCJ010000456         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_5072, whole genome shotgun sequence.
ACCESSION   DASUCJ010000456 DASUCJ010000000
VERSION     DASUCJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5072"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..548)
                     /locus_tag="VFU90_05470"
     CDS             complement(132..548)
                     /locus_tag="VFU90_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479259.1"
                     /translation="MDDFRTELTAVVDEMRAAGIEPTFLVVDLDGIGYVKQARGAESA
                     DTFRASATDAIAAAAGGCDTFRYGEERIVAILPGYTRLKTFALIERLRRALPLLGQSF
                     DCPLQVEFDVLEYDEQHGVPALVAQLAALTRNRDVA"
     gene            complement(645..>1166)
                     /locus_tag="VFU90_05475"
     CDS             complement(645..>1166)
                     /locus_tag="VFU90_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479260.1"
                     /translation="AIHFPLQDNERVLLVCRRHWIYLWPKTLLYALFAIVPPIAAGIL
                     LSMADQYGGMAARVFWIASAVWMLYWAVRMLLNWYRYHNDIWVITNQRLIDSNRPTPF
                     NLKLSTADLVNVQDMTVEREGLLQTMLDYGDIVCQTAADMQEFRLPGIPKPREVQLLV
                     DRERDRERTRVAG"
BASE COUNT          197 a          360 c          422 g          187 t
ORIGIN      
        1 cgagtgctta gtttgggtca ttctgcgccc cggaaacgtt gagatcaagc gcaacgcggg
       61 acgcgggtcg cggctgcgga cggtaacggg gattgtgggt gatcccgttc gattacgtca
      121 aggcgaaggg attacgcgac gtcgcgatta cgcgtgaggg ccgcgagctg ggcgaccagc
      181 gcggggacac cgtgctgctc gtcgtactcg aggacgtcga actcgacctg gagcgggcag
      241 tcgaacgact gcccgagcag cggcagcgcg cggcggaggc gctcgatcag ggcgaaggtc
      301 ttgaggcgcg tgtacccggg aaggatcgcg acgatgcgct cctcaccgta gcggaatgtg
      361 tcgcagccgc cggcggccgc cgcgatggcg tcggtcgcgg acgcgcggaa ggtgtccgcc
      421 gattcggcgc cacgcgcctg tttcacgtag ccgatgccgt ccaagtcaac gacgaggaac
      481 gtcggctcga tcccggccgc gcgcatctca tcgacgacgg cggtcagctc cgtgcggaag
      541 tcgtccatgc acgtagtatc ggcgtgccgc cgcggggccg ggagcagggt tgtcacctag
      601 ggacgaggcc accgcgcctt atcgtgccgt gatgcgaccg acggctatcc ggcgacgcgc
      661 gtccgctcgc ggtcgcgctc gcggtcgacg agcaactgca cctcgcgcgg cttcgggatg
      721 ccgggcaggc ggaactcctg catgtctgcc gccgtctggc agacgatgtc gccgtagtcg
      781 agcatcgtct gcaggaggcc ctcgcgctcg acggtcatgt cctgaacgtt cacgaggtcc
      841 gcggtcgaga gcttcaggtt gaacggtgtc ggacggttgc tatcgatgag ccgctggttc
      901 gtgatgaccc agatgtcatt gtggtagcga taccagttga gcagcatccg gacggcccag
      961 tagagcatcc acaccgccga cgcgatccag aagacgcgcg ccgccatgcc accgtactgg
     1021 tcggccatcg acagcaggat gccggcggcg atcggcggca cgatcgcgaa gagcgcgtag
     1081 agcagcgtct tcggccagag gtagatccag tgccggcggc agacgagcag cacgcgctcg
     1141 ttgtcctgca gcgggaagtg gatcgc
//
LOCUS       DASUCJ010000457         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_325461, whole genome shotgun sequence.
ACCESSION   DASUCJ010000457 DASUCJ010000000
VERSION     DASUCJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325461"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFU90_05480"
     CDS             <1..1059
                     /locus_tag="VFU90_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479261.1"
                     /translation="DTGRDGALHLTVHNVGLAIGNARYVDGIRLNFRDSGHYVVHGLN
                     ATIWTPYQHSDGLVQGIALGVPLTGARRIDGVGLGIGLGADDDLSGVAVGVLGAGAGK
                     SVHGILFGGLGAGSGSDVEGLAAGGLGVGAGRDVEGVIIGGLGVGAGHDVTGVSLAGL
                     GSGAGNRVRGLSVAGLGVGAGESLDGISIAGVGVGAGQSVRGLTLAGVGVGAGDNIVG
                     ITVAGLGVGAGKRVKGLTIAGIAVGAGGSIDGITLAGLAVGAPRLHGFVASLVAGGNY
                     VKGGVIAPAWLRISREGSFTGFSLSAFNQVKGTQHGLTLGIFNFARELHGVQIGLLNY
                     AGNNSGLAKLLPLVNAHH"
     gene            1112..1330
                     /locus_tag="VFU90_05485"
     CDS             1112..1330
                     /locus_tag="VFU90_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479262.1"
                     /translation="MDALVKARFDLQHAAHDFGGHRVKALQATDAALEQLKICLEYDG
                     QHRPRSIEAGRVSPAGRASTSRRVVLPA"
     gene            complement(1327..1491)
                     /locus_tag="VFU90_05490"
     CDS             complement(1327..1491)
                     /locus_tag="VFU90_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479263.1"
                     /translation="MENITPICRAAALPASARRALSGDDDFRTALEWNADRRALRSGS
                     GGAMRDEDLR"
     gene            1529..2497
                     /locus_tag="VFU90_05495"
     CDS             1529..2497
                     /locus_tag="VFU90_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479264.1"
                     /translation="MIEAFPVGRRLAFDPARGRLWVVCTHCERWNLSPLDERWEAIDD
                     CERRFRATRLRMSTDNIGLARIADGMTLVRIGQPLRPELAAWRYGDQFGRRRRFRVAQ
                     AGVGVTAVGATLAGGAAVGVGVGGIAFAVTQWGRALLYGSPSAIVARIPIGNGIVAPV
                     RRADLDRLAVRGGTSATDWALCVRYAASHNNTPPVELEGEAAQRAAAMLLPVVNRFGG
                     SRVQIAAAVRVLEEAVTPLGVFALTVRQQDHALVLPLPVPVRLALEMAAHEERERRAL
                     EGELAELEREWREAEEIAEIADSLLLPTHVDEVLGGMRHRVEPTRT"
     gene            2494..>3155
                     /locus_tag="VFU90_05500"
     CDS             2494..>3155
                     /locus_tag="VFU90_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479265.1"
                     /translation="MTSITAPRIAGDHREAVLATFLGWTLDAFDFFLVVFALTSIAGE
                     FGRTDKALALSLTLTLAFRPVGAFIFGLMADRYGRRIPLMIDLVFFSVIEVLTGLAPN
                     FTTFLILRALFGIGMGGEWGVGASLAMEKAPTRLRGVLSGLLQEGYAVGYLLAAVCFF
                     FVFPRWGWRPMFFIGGLPALLALFIRVRVKESEVWQRTRHESWSGLGRAIVGNWKLFA
                     YLV"
BASE COUNT          440 a         1000 c         1142 g          573 t
ORIGIN      
        1 gacaccggtc gtgacggcgc gctgcatctc accgttcaca atgtcggact cgccatcggc
       61 aacgcgcgct acgtcgatgg catccgcctc aactttcgcg acagcggcca ctacgtcgtt
      121 cacgggttga acgcgacgat ctggacgccg taccagcaca gcgatgggct cgtccaaggg
      181 attgcgctcg gcgtgccgct caccggagcg cggcgcatcg acggcgtcgg tctcggcatc
      241 gggctgggcg ccgacgacga tctctccggt gttgcggtgg gtgtcctcgg cgccggagcc
      301 ggcaagtcag ttcacgggat cctgttcggc ggactcggcg ccgggtccgg atccgacgtg
      361 gagggtctcg ccgcgggcgg actgggggtc ggcgccggcc gggacgtgga gggcgtgatc
      421 attggtggac tcggggtcgg cgcggggcac gatgtgacgg gcgtcagcct ggccggactt
      481 ggcagcggcg ccgggaatcg cgtgcgcggg ttgagcgtgg ccggcctcgg cgtcggtgcg
      541 ggagagagcc tggacgggat atccatcgcc ggcgttggcg tgggcgcggg gcaaagcgtg
      601 cgcggcttga cgctcgccgg cgtcggcgtc ggcgccgggg acaacatcgt gggcatcacc
      661 gtcgccggct tgggcgttgg cgccggcaaa cgggtcaagg gcctcacgat agcaggaatc
      721 gctgtcggag ccggggggtc gatcgacggg atcaccctgg caggtctggc cgtcggcgcg
      781 ccgaggctgc acggattcgt ggccagtctg gttgcgggcg gcaactacgt gaaaggaggg
      841 gtgatcgctc ctgcgtggct gcgaatttct cgagaaggat cgttcaccgg gttttcactg
      901 agcgcgttca accaggtgaa gggcacacag cacgggctca cgctcggcat cttcaatttc
      961 gcgcgcgagc tgcacggcgt tcaaatcggc ctgctcaatt acgccggcaa caactccggt
     1021 ctcgcgaagc tgctgcctct cgtgaacgcg catcactgat ccgctgggat gagggtttct
     1081 cgacgagcgt catcccgcga tcaatgcggc ggtggacgcg ctggtaaagg cgcgcttcga
     1141 tctgcagcat gcggcgcacg atttcggcgg tcaccgtgtg aaggctctcc aggctaccga
     1201 cgctgcgctc gagcagctca agatctgcct cgagtacgac gggcagcatc gtccccgatc
     1261 gatcgaggcc ggccgcgtga gtccggccgg ccgggcctcg acatcgaggc gcgtggttct
     1321 tcccgcctaa cgcagatctt catcgcgcat cgccccaccg ctgcccgagc gcaatgcgcg
     1381 acggtcagca ttccattcga gcgccgtacg gaagtcgtca tcgcccgaca atgcgcgtcg
     1441 cgcgctcgcg ggcagcgccg cggcgcgaca tattggggtg atgttctcca cgtgcatctt
     1501 ctgccacgcg tcactgggga caaatcccgt gatcgaggcg ttcccggtgg gtcggcggtt
     1561 ggcgttcgat cccgcgcggg gacggctctg ggtagtctgc acgcactgtg aacggtggaa
     1621 cctctcgccg ctcgacgagc gatgggaggc catcgacgat tgcgagcgga gattccgcgc
     1681 cacgcggctg cgcatgtcga cggacaacat cggcctggcg cgcatcgcgg acggcatgac
     1741 gctcgtgaga atcggacagc cgcttcgtcc ggagcttgcg gcgtggcgct acggcgacca
     1801 attcggccgg cgtcgtcgat ttcgcgtcgc gcaagctggc gtcggcgtga ccgctgtcgg
     1861 agcgacgctc gccggcggcg cggcggttgg tgtcggtgtc ggcggtatcg cattcgcggt
     1921 cacgcagtgg ggacgggcgc tcctgtacgg atcgccgtcg gcgatcgtgg cgcgcattcc
     1981 catcggcaac ggcatcgtcg cgccggtgcg gcgcgcggat ctcgatcggc tggcggtgcg
     2041 cggcgggacg agtgcaaccg actgggcgtt gtgcgtccga tatgcagcgt cgcacaacaa
     2101 caccccgccg gtcgagctgg aaggtgaggc tgcccaacgc gcggcggcga tgctcctgcc
     2161 ggtggtgaac cggttcgggg gatcgcgcgt ccagatcgcg gcggccgtcc gggtgctcga
     2221 ggaggccgtg actccgttag gcgtgttcgc gctcaccgtg cggcagcagg accacgcctt
     2281 ggtgcttccc ttgcccgtcc ccgtgcggct ggcgctcgag atggcggcgc acgaggagcg
     2341 ggagcgccgc gcgctcgagg gtgagttggc tgagctcgag cgcgaatggc gggaagccga
     2401 ggaaatcgcg gagatcgcgg attcgctgtt gctgccgacg cacgtcgacg aggtgctcgg
     2461 cggcatgcga catcgcgtcg agccgacccg cacgtgacgt cgatcaccgc accccgcatt
     2521 gccggcgacc atcgcgaagc ggtactcgcc acgttcctcg gctggacgct cgacgcattc
     2581 gatttcttcc tcgtcgtgtt cgcgctcacg tcgatcgccg gcgaattcgg acgcaccgac
     2641 aaggcgctcg cgctctcgct cacgctcacg ttggcgttcc gccccgtggg cgctttcatt
     2701 ttcgggctga tggcggatcg ctacggccgg cgcattccgc tgatgatcga cctcgtgttt
     2761 ttctcggtga tcgaagtgct caccggactc gcgcctaact tcacgacgtt tctcatcctg
     2821 cgcgcgctct tcggcatcgg gatgggcggc gaatggggcg tcggcgcatc gctggcgatg
     2881 gagaaagcgc cgacgcgctt gcgcggcgtg ctgtccgggt tgctgcagga gggctacgcc
     2941 gtgggatatc tgctcgcggc ggtctgcttc tttttcgtct tcccgcgctg gggatggcgt
     3001 ccgatgttct tcatcggcgg cctgcctgcg ctgctcgcgc tgttcatccg cgtgcgcgtc
     3061 aaggaatccg aagtgtggca gcgcacgcgt cacgagagct ggtcgggtct tggccgcgcg
     3121 atcgtcggca actggaagtt gttcgcgtac ctcgt
//
LOCUS       DASUCJ010000458         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1059453, whole genome shotgun sequence.
ACCESSION   DASUCJ010000458 DASUCJ010000000
VERSION     DASUCJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059453"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /gene="ppk1"
                     /locus_tag="VFU90_05505"
     CDS             complement(<1..1162)
                     /gene="ppk1"
                     /locus_tag="VFU90_05505"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU5479266.1"
                     /translation="MTRDADLDLQEDEADDLLRAIESELQRRRFGEPVRLEIERGMPE
                     YLREFLLKALELSSVDCYEIDGMMGLGDLWTIVNLDYKQLHDPPFTPAIPKRLIGVTD
                     MFAAIREGDVLLHHPYESFDPVVQFLQHAAEDDKVLAIKATLYRTSGKNSPIVRALLQ
                     AAEEEKQVAVVIELKARFDEENNIEWARRLERAGVHVVYGFAGLKTHGKSILVVRREE
                     DGIRRYMHFGTGNYSEKSARLYTDLSLFTCRPELGADVSQLFNALTGFSKITDYEDLL
                     VAPVNLRRELSALIDRETEHAREGRPSGIRAKLNAITDGEIGRLLYRASQAGVPVDLQ
                     VRGMCVVRPGVPGVSERIRVRSIVGRFLEHSRIYVFENGGDREVFIGSADWMG"
BASE COUNT          219 a          379 c          355 g          225 t
ORIGIN      
        1 ggcccatcca gtccgcggag ccgatgaaga cctcgcggtc gccgccgttc tcgaacacgt
       61 agatgcgtga gtgctccagg aagcgcccca cgatactgcg tacgcgaatt cgctcgctca
      121 cgcccggcac gcccgggcgg acgacgcaca tacctctgac ctgcagatcg acgggaacgc
      181 cggcctgtga ggcgcgatag agcagccgcc cgatctcgcc gtcggtaatc gcgttgagct
      241 tcgcgcgaat gccgctgggg cgtccctcgc gcgcgtgctc ggtttcgcgg tcaatcagcg
      301 cgctgagctc gcgccgcagg ttgaccggcg cgacgagcag gtcctcatag tcggtgatct
      361 tcgagaagcc ggtcagcgcg ttgaaaagtt gggagacgtc ggcgccgagt tccggacggc
      421 acgtgaacag gctcaaatcc gtgtacaggc gcgcggactt ctcgctgtaa ttgccggtgc
      481 cgaaatgcat gtagcggcga atgccgtctt cttcgcggcg caccactaaa atcgacttgc
      541 cgtgtgtttt caaaccggca aagccgtaca cgacgtgcac gccggcgcgc tccaggcgcc
      601 tcgcccattc gatgttgttc tcttcatcga agcgcgcctt gagctcgatc accaccgcaa
      661 cttgcttttc ttcttcggcc gcctgcagca acgcgcgaac gatcggcgaa tttttgcccg
      721 acgtacgata gagcgtcgcc ttgatcgcga gcactttatc gtcctctgcg gcgtgttgca
      781 agaattgtac gaccggatcg aacgattcgt acgggtggtg cagcagcacg tccccttcgc
      841 gaatcgcggc gaacatatcg gtcaccccga tcaagcgctt cggaatcgcc ggcgtgaacg
      901 gcggatcgtg cagctgctta tagtcgaggt tcacgatggt ccaaagatcg cccaggccca
      961 tcatgccgtc gatctcataa cagtcaacgc tgctgagctc gagcgctttg agcaagaact
     1021 cccgcagata ttccggcatg ccgcgctcga tctccagacg aacgggctcg ccgaagcggc
     1081 ggcgctgcag ctccgattcg atcgcgcgca gcaagtcgtc ggcttcgtct tcctgcaaat
     1141 cgaggtcggc gtcgcgcgtc acgcggaaca gatacgag
//
LOCUS       DASUCJ010000459         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1059460, whole genome shotgun sequence.
ACCESSION   DASUCJ010000459 DASUCJ010000000
VERSION     DASUCJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059460"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..985)
                     /locus_tag="VFU90_05510"
     CDS             complement(41..985)
                     /locus_tag="VFU90_05510"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEU5479267.1"
                     /translation="MPIEVTPETYIRAETDRIFHNLEQMAGGVNRLFHLRAPLPLDQQ
                     DVVRMNLDTLYSVAIVDTQGGATLTLPPVPDRRFMSALVIDNDHYAPAVYYRPGTYQL
                     PGNTKYVQVVIRVQVNAKDPADVAVANQLQDGVVIRAHSAEPMAPDRWDPASLTALRV
                     QYEKDASAYGSFKGMMGPRGSVDEKTRHIAAAAAWGLNPETDATYLNYAGNAPANGCY
                     TATYHVPPNDAFWSITVYGNDGYIKSDNALLNENNVEFNTDGTFTVYFGSEASCGSKP
                     NRLDISPGWNFLMRVYRPGKAVLDGSYTLPKAVPVALR"
     gene            1097..>1973
                     /locus_tag="VFU90_05515"
     CDS             1097..>1973
                     /locus_tag="VFU90_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479268.1"
                     /translation="MTVFARTRYVAAATALALLLAGCSGSQSSHALPLGMTNAGNQAA
                     SRNAGAVELLRGGMTQSRLLELQLAGRLEAPVPPKVLRAQLAQPSAGRPHFALHPDAR
                     VAIWASNTNFNYLLGQDATGSHTEIAVDASQNSCYSPIALKVDHARNLWVACELTSPS
                     TVSGALQEYSPTGTLKKQYLPQCPKNISGCSGFGGYGFDSGLDAQHDVFAALNLYSYQ
                     VCTPSCQTTLGAGFEWWPKGAGTATKPTLISVSANCSPICGVGYMDVDSNGNLWFTLA
                     GYDPSGNFGFGLGEIK"
BASE COUNT          359 a          657 c          584 g          373 t
ORIGIN      
        1 cctgagcaga gcgccgcagg cgcgcagtcg aaggacgcgc tcaccgcaac gcaaccggca
       61 cggctttcgg aagcgtgtag ctgccgtcca gcacggcctt tcccgggcgg tagacgcgca
      121 tcaggaaatt ccaacctgga ctaatatcga gccggttcgg ttttgatccg cacgacgctt
      181 ccgagccgaa gtataccgtg aacgtcccgt ccgtgttgaa ctcgacgttg ttctcgttga
      241 gaagcgcgtt gtcgctcttg atgtagccgt cgttaccgta gacggtaatc gaccagaacg
      301 catcgttcgg cggcacgtgg tacgttgcgg tgtaacagcc gttcgccgga gcgtttccgg
      361 cgtagttcaa atacgtcgca tccgtttccg ggttcaaacc ccacgctgca gcggccgcga
      421 tgtgacgcgt cttctcgtcg accgacccgc gtgggcccat catccctttg aagctgccgt
      481 acgcggacgc gtccttttcg tactgcaccc gcagggccgt cagcgaagcg ggatcccagc
      541 gatccggcgc catcggctcc gcgctgtgag cccgaatgac gacgccatcc tggagctggt
      601 ttgcaaccgc gacgtccgcc gggtccttcg cattgacctg aacgcggatg acgacttgca
      661 cgtattttgt attgccggga agctgatagg taccgggcct gtagtacacc gcgggcgcgt
      721 agtggtcgtt gtcgatcacc agcgccgaca tgaagcggcg gtcgggaacg ggcggaaggg
      781 taagtgtggc gccgccctgc gtatcgacga tcgcgacgga atacagggtg tctaagttca
      841 ttcggacgac gtcctgttgg tcgagcggta gcggcgctcg cagatgaaac aatcggttga
      901 cgccgccggc catctgctcg agattatgga aaatgcggtc ggtttcggcg cgaatgtacg
      961 tctccggcgt gacctcgatc ggcatcgttt cctccgcccc gtgccggggg ccccatcata
     1021 ctccatttct gccatttttc ggaaagcatc gcacatcctc aagaggccag cgcggtcttg
     1081 tcgataagcc ttacgcatga ccgttttcgc acgtacccga tacgtcgccg ccgcgacggc
     1141 cctcgcgctg ctgctcgccg gctgctccgg ctcccagagc tcgcatgccc ttccgctcgg
     1201 catgaccaat gccggcaacc aagcagcttc gcgcaacgcc ggcgccgtcg aactcctacg
     1261 cggcggcatg acgcaatcgc gactgctcga gctgcagctc gccggccgcc tcgaagcccc
     1321 cgttccgcca aaagtgctgc gcgcgcaact cgcgcaaccg agcgccggcc gcccgcactt
     1381 cgcgctgcac cccgacgcgc gcgtcgcgat ctgggcctcg aacacgaact tcaattactt
     1441 actcggtcaa gatgcgaccg gctcgcacac cgagatcgcc gtcgacgcat cccagaactc
     1501 gtgctactcc ccgatcgcgc tcaaggtcga ccacgcgcgc aacctctggg tcgcctgcga
     1561 gctgacgtcg ccgagcacgg tcagcggcgc cttgcaagaa tatagcccta ccggcacgtt
     1621 gaaaaaacaa tacctgccgc aatgcccgaa gaacatttcg ggctgctcgg gcttcggcgg
     1681 ctacggtttc gatagcgggc ttgacgccca gcacgacgtc ttcgctgcac tgaacctcta
     1741 cagttatcaa gtttgcaccc cgagctgcca gacgacgctc ggcgcaggct tcgaatggtg
     1801 gccgaagggc gccggcacgg cgaccaaacc gacgctcatc tcggtcagcg ccaactgttc
     1861 gccgatctgc ggcgtcggct acatggacgt cgattccaac ggcaaccttt ggttcacgct
     1921 cgccggatac gatccgagcg gcaacttcgg tttcgggcta ggcgaaatca aga
//
LOCUS       DASUCJ010000460         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1472035, whole genome shotgun sequence.
ACCESSION   DASUCJ010000460 DASUCJ010000000
VERSION     DASUCJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472035"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFU90_05520"
     CDS             complement(<1..227)
                     /locus_tag="VFU90_05520"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEU5479269.1"
                     /translation="MRNLVQSVVAIAALTVVIGFGYPALMTSFASVAFPHQAAGSLIE
                     VNGAVVGSKLAAQQFTSPKYFMERPSGTTPA"
     gene            complement(227..>1527)
                     /gene="kdpA"
                     /locus_tag="VFU90_05525"
     CDS             complement(227..>1527)
                     /gene="kdpA"
                     /locus_tag="VFU90_05525"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU5479270.1"
                     /translation="LAVQNFVSAGVGLAVLVAVVRGIARRSSTELGNFWRDLSRTIAY
                     VLVPLSVILAVALIWQGVPQTFHAHAAATTLQGAHQTIARGPAGSQIAIKQLGTNGGG
                     YYNSNSAVPFENPTGLTNFLEMLSILLIPAACVFMFGKMVGARRHGWMVFAAMFAVFA
                     IGVAINLPAEQHGSSVLRKSGVNITMSGSQAGGNMSDKEVRFGQAISAVWTVATTDAS
                     NGSVNNGHDAQTPVGGAVPLVNIFLGEVIFGGVGSGLYGMFFYILIAVFIAGLMVGRT
                     PEWLGKKIEAREIKLAALGALFVPTMALAMTAVAIVTKSGLASIYNPGAHGFTEALYA
                     YTSQSNNNGSAFAGYGATSFSATLGTVALYFGRFVPLLSALAIGGSLAKKKIVPASAG
                     TFRTDGPTFVVLLVGVIALTAGLMILPALTLGPIVEGLMH"
BASE COUNT          274 a          494 c          527 g          232 t
ORIGIN      
        1 taggccggcg tcgttcctga cggccgctcc atgaagtact tcggcgaggt gaactgctgt
       61 gccgcgagct tcgacccgac cacggccccg ttcacctcga tgaggcttcc tgccgcctgg
      121 tgtgggaatg cgaccgacgc gaagctggtc atgagcgcgg gatagccgaa cccaatcacc
      181 acggtgaggg cggcgatcgc gacgaccgac tggacgaggt tgcgcatcag tgcatcagcc
      241 cttcgacgat cgggccgagt gtgagcgcgg gcaggatcat caagccggcg gtgagcgcga
      301 tgacgccgac gagcaggacg acgaacgtcg gcccgtcggt gcggaacgtc cctgctgagg
      361 ccggaacgat cttcttcttc gcgagcgatc cgccgatggc gagcgcggag agaagcggga
      421 cgaagcggcc gaagtagagc gcgaccgtgc cgagcgtcgc ggagaagctc gtcgccccat
      481 agccggcgaa cgcgctgccg ttgttgttcg actgcgatgt gtacgcgtac agcgcctcgg
      541 tgaagccgtg cgcgccgggg ttgtagatcg acgcgagccc ggatttcgtc acgatcgcca
      601 cggccgtcat ggcgagggcc atcgtcggta cgaacaacgc gccgagcgcg gcgagcttga
      661 tctcgcgcgc ctcgatcttc ttgcccagcc actcaggcgt ccggcccacc atcaatccgg
      721 cgatgaacac cgcgatgagg atgtagaaga acatcccgta cagcccagac ccgacaccgc
      781 cgaagatcac ttccccgagg aagatgttca cgagcggcac tgcgccgccg accggcgtct
      841 gtgcgtcgtg gccgttgttg accgagccgt tcgaggcgtc ggtcgtcgcg accgtccaga
      901 cggcggagat ggcctggccg aagcgcactt ccttgtcgga catgttgccg cccgcttggc
      961 tgccgctcat ggtgatgttc acgcccgact tgcgcagcac ggacgacccg tgctgctcgg
     1021 ccggcaggtt gatcgccacg ccgatggcga acaccgcgaa catcgcggcg aacaccatcc
     1081 agccgtgacg gcgcgcgccg accatctttc cgaacatgaa gacgcacgcg gccggaatga
     1141 gcaggatcga gagcatctcg aggaagttcg tgagcccggt cgggttctcg aacgggacgg
     1201 ccgagttcga gttgtagtag ccgccgccgt tcgttccgag ctgcttgatc gcgatctgcg
     1261 accccgccgg gccgcgggcg atggtctgat gcgcgccctg cagcgtcgtc gcggccgcgt
     1321 gcgcatggaa cgtctgtggc acgccctgcc agatgagagc gaccgcgagg atgaccgaaa
     1381 gcggaacgag gacgtacgcg atcgtgcgag agaggtcgcg ccagaagttc ccgagctcgg
     1441 tgctggaacg gcgggcgatg ccgcgcacga ccgcgacgag aaccgcgagg ccgacgccgg
     1501 ccgagacgaa gttctgcacc gcgagcc
//
LOCUS       DASUCJ010000461         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_845542, whole genome shotgun sequence.
ACCESSION   DASUCJ010000461 DASUCJ010000000
VERSION     DASUCJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845542"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFU90_05530"
     CDS             <1..202
                     /locus_tag="VFU90_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479271.1"
                     /translation="EIAELLAPAEARSAESVAHAAAAKLDEVDAKLRELMALRCRLRQ
                     LLRVCAHGDTTECAALQLVRTA"
     gene            228..563
                     /gene="trxA"
                     /locus_tag="VFU90_05535"
     CDS             228..563
                     /gene="trxA"
                     /locus_tag="VFU90_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5479272.1"
                     /translation="MSLHATTDESFARDVLASPMPVLVEFTADWCPPCRMIVPVLQKI
                     ADDEASRLRVVSIDVDANPVTTRAYGVMSMPTLALFVDGKPVVQFVGAKPRTTIMRLI
                     ESHLPVAAG"
     gene            complement(640..1020)
                     /locus_tag="VFU90_05540"
     CDS             complement(640..1020)
                     /locus_tag="VFU90_05540"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5479273.1"
                     /translation="MSDLIKLSGTTVNAPDAIALARFYAEITGGVARGDENWALVEGP
                     SGNIGFQQVHDYRPPTWPEGEVGMQMHLEFFVDELEATEARVLAAGATRFEYQPNADH
                     CLVYADPAGHPFCLSMWDAPRARD"
BASE COUNT          228 a          461 c          468 g          238 t
ORIGIN      
        1 cgaaatcgcc gagctgctgg cgccagcgga ggctcgatca gctgagagcg tcgcgcacgc
       61 ggccgccgcg aaactcgatg aagtcgacgc aaaactgcgc gagctcatgg cgttgcgttg
      121 ccgacttcgg cagttgctgc gcgtctgcgc gcacggcgac acaacggagt gcgcggcgct
      181 gcaactggtg cgcaccgcgt gaactgcgga cctttgaggg agacgacatg tcactgcacg
      241 ccacgaccga tgagtcgttc gcgcgcgacg tgctggccag cccgatgccg gtgctcgtgg
      301 agttcaccgc cgactggtgc ccgccgtgcc ggatgatcgt gccggtgctg cagaagatcg
      361 cggacgacga agcgtctcgg ctgcgcgtcg tctcgatcga cgttgacgcg aatccggtga
      421 cgacgcgcgc gtacggcgtg atgagcatgc cgacgctggc gttgttcgtc gacgggaagc
      481 cggtggtgca gttcgtcggc gctaagccgc ggacgacgat catgcggctc atcgagtcgc
      541 atctgccggt ggctgcaggc tgatcgggcg cgagcgcgac acgcccggga tgcggttcag
      601 ctgaacgcgg ctgctcggca cagaacgatg gaggtcgcgt cagtcgcggg ctctgggtgc
      661 gtcccacatt gacaggcaaa acggatgccc ggccgggtcg gcgtaaacga ggcagtggtc
      721 ggcgttgggc tgatactcga atcgggtagc gccggccgcc agcacgcgtg cctcggtcgc
      781 ctccagttcg tcgacaaaga attcgaggtg catctgcatc cccacctcgc cctccggcca
      841 ggtgggcggc cgatagtcgt ggacctgctg gaacccgatg ttgccgctcg gcccttcgac
      901 gagcgcccag ttctcgtcac ccctcgcgac cccgccggtg atctcggcat agaaccgcgc
      961 gagcgcgatt gcgtcggggg cgttgacggt cgttccgctg agcttgatca gatccgacat
     1021 caaccgatct ccgtccgctc gcacatgggc gtgagctctt cccagcgttg cttccagccg
     1081 ctgaccggca tcggagtccg agctctccgt acggaccggt cgtcaacctc cagcgaccga
     1141 ccgagatcct cgaagtggac ccgccaaccc gaggcgtgaa acgggaagcg cgtcatcggc
     1201 aggccacgct cctcgatcac ccgccggatc tttgaacgct cctcactatc gtcgcatcgc
     1261 ggtgccgcgt ctccggcatt gccgccgtga cacacggcgc gcgtcacgca ggcttgagcg
     1321 cggcgcgccc gggatgcggt tcagctgaac gcggttatcc tcaggacgag atgtcgactg
     1381 actcagatca ccttc
//
LOCUS       DASUCJ010000462         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_524530, whole genome shotgun sequence.
ACCESSION   DASUCJ010000462 DASUCJ010000000
VERSION     DASUCJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524530"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1090)
                     /gene="ftsZ"
                     /locus_tag="VFU90_05545"
     CDS             complement(<1..>1090)
                     /gene="ftsZ"
                     /locus_tag="VFU90_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5479274.1"
                     /translation="GGNAVNRMIEEHLEGVEFISVNTDAQALLNSKSDVKIQIGKKLT
                     RGLGAGARPEIGRQAIEENRDEVGRAMTNADLVFVTCGMGGGTGTGAAPIVCEMAREA
                     GALTVGIVTKPFLFEGRKRMRQADAGISEMRKYVDTMIVVPNERLLAVVGKGIPFGDA
                     LKKADEVLLHATQGISNLISVTGLVNVDFADVRTVMQSGGSALMGTGIGRGENRAMEA
                     AQQAISSPLLDNISVGGATGVLINIMGGNDLTLGEVHQINEIVHDAVGDDAEIIFGAV
                     NEPAMQGEIRVTVIATGFDRALQSGGVAEPPSRVMMPTTPVLPFPAQRQRQPVVPPRA
                     TPPIERRASQPGTPSDKTSQDLSDMEIPT"
BASE COUNT          150 a          392 c          342 g          206 t
ORIGIN      
        1 cgtcggaatc tccatgtcgc tgagatcctg cgacgtcttg tcggacggcg ttccgggctg
       61 cgacgcgcgg cgctcgatgg gcggcgtcgc gcgcggcggc acgacaggct gccgttgccg
      121 ttgggccggg aacggcagca ccggtgtcgt cggcatcatc acgcgcgacg gcggctccgc
      181 cacgccgccg gactgcaggg cgcggtcgaa cccggtcgcg atcacggtga cgcggatttc
      241 gccttgcatc gccggctcgt tcaccgcgcc gaagatgatt tcggcgtcgt cgcccacggc
      301 gtcgtgcacg atttcgttga tctggtgcac ttcgcctaac gtgaggtcgt tgccgcccat
      361 gatgtttatc aggacgcccg tcgcgccgcc taccgagatg ttgtcgagca gcggcgagga
      421 gatggcctgc tgcgccgctt ccatcgcccg attctcgccg cggccaatgc ccgtgcccat
      481 gagggccgag ccgccgctct gcatcacggt gcggacgtcg gcgaagtcga cgttcacgag
      541 gcccgtcacg ctgatcaggt tcgagatgcc ctgcgtggcg tgcagcagca cttcgtcggc
      601 cttcttgagc gcgtcgccga agggaatgcc cttcccgacc acggccagca accgctcatt
      661 gggcacgacg atcatcgtgt ccacgtactt gcgcatctcg ctgatgccgg cgtccgcctg
      721 gcgcatgcgc ttccgtccct cgaacaggaa cggcttggtc acgatgccca ccgtgagcgc
      781 gcccgcctcg cgcgccatct cgcacacgat cggcgccgcc ccggtgcccg tccccccgcc
      841 cataccgcac gtcacgaaaa cgaggtcggc gttcgtcatt gcgcgcccga cctcgtcccg
      901 gttctcctcg atggcctgcc gaccgatttc cggccgcgct ccggcgccga gcccgcgcgt
      961 cagcttcttc ccgatctgga tcttgacgtc cgacttcgag tttagcaggg cctgcgcatc
     1021 cgtgttgacg gagatgaact cgaccccttc gagatgttcc tcgatcattc gattgacagc
     1081 gttaccgccg
//
LOCUS       DASUCJ010000463         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_386908, whole genome shotgun sequence.
ACCESSION   DASUCJ010000463 DASUCJ010000000
VERSION     DASUCJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386908"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU90_05550"
     CDS             <1..184
                     /locus_tag="VFU90_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479275.1"
                     /translation="FYGKNNNVHLADKQALITFMVSANAARIIKKLEYTPLSGSVHTA
                     IINAMNGSGSKAPCLQ"
     gene            262..1005
                     /locus_tag="VFU90_05555"
     CDS             262..1005
                     /locus_tag="VFU90_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEU5479276.1"
                     /translation="MTHKFRAVAAAAALLAAGCGGSGSSAAIPGDSNRISVRFVEGAP
                     VLETLVNGVPTSIGEAYLAVDAETIASSFPYGDVTPFVTRPAGVQSIEARDSLGYSVG
                     PLKTDALPAGKSYTVVVAGTYPNYRALAFLETAGDGGASLAVYEASPAYPVADFGTFR
                     ASRGNGFKKLGSVRLGNVAAVSLGRTVANLGAYAGKGTVPIGTLTVRAVNQFDQRNAL
                     PFHSASRFSIFLLDPAAGSGGPLFGSLDQ"
     gene            1002..>1283
                     /locus_tag="VFU90_05560"
     CDS             1002..>1283
                     /locus_tag="VFU90_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479277.1"
                     /translation="MNGKTAFALLTMLAAAATAISGCGGASGPAPVALASPPLPTPAP
                     PARRLYADHNGTLYEYTLPLQANAKPVDVFVESPGAAIAPQIAVDANGTI"
BASE COUNT          215 a          446 c          398 g          224 t
ORIGIN      
        1 gttctacggg aaaaacaata acgtccacct cgccgacaag caggccctga tcacgttcat
       61 ggtctcggcc aatgcggcga gaatcatcaa gaaactcgaa tacaccccgc tttccggatc
      121 cgttcacacc gctattatca acgccatgaa cggcagcgga agcaaggcac cctgtctcca
      181 gtaaccctgg agccctcgtc gtcacgcgcg ccgctccggg cggcgcgcgt gtgcgacatc
      241 ttcctgcgag gctctcgttc gatgactcat aagtttcgcg cggtggctgc ggctgcggcg
      301 ctgctggccg ccgggtgcgg cggctcgggc tcgtcagccg cgattccggg cgacagcaac
      361 cgcatttcgg tgcgtttcgt cgagggagcg ccggtcctcg aaacgctggt caacggcgtg
      421 ccgaccagca tcggcgaggc ctatctcgcg gttgacgccg agacgatcgc atcgtcgttt
      481 ccgtacggcg acgtcacgcc gttcgtcacg cggccggcgg gcgtgcaatc gatcgaggca
      541 cgcgatagcc tcggctactc ggtcgggccg ctcaaaaccg atgcgttgcc ggccgggaaa
      601 agctacaccg tcgtcgtggc ggggacgtat ccgaactacc gcgcgctcgc gtttctggaa
      661 accgccggcg acggcggcgc atcgctggcc gtctatgaag cctcgcccgc ctatccggtt
      721 gcagatttcg gaacctttcg cgcctccagg gggaacgggt tcaaaaagct cggcagcgtt
      781 cgtctcggca acgtcgccgc cgtctcactc ggaaggacgg tcgcgaatct cggcgcctat
      841 gccggtaagg gaaccgttcc catcggcacg ttgacggtgc gcgcggtaaa ccagttcgat
      901 cagcgcaacg cactgccgtt tcactccgcg agccgctttt cgattttctt actcgacccc
      961 gccgccggca gcggcggccc gctattcgga agtctggatc aatgaacggc aagaccgcgt
     1021 tcgcgcttct gacgatgctc gccgctgccg cgaccgcgat ctcggggtgc ggtggcgcga
     1081 gcggcccggc gccggtcgcg ctggcgtcgc cgcctctgcc cacgcccgcg ccgcctgcac
     1141 gccggctcta cgccgatcac aacggcacgc tgtacgaata tacccttccg ctgcaagcga
     1201 acgccaagcc ggtcgacgtc ttcgtcgaat cgccgggcgc cgcgatcgcg ccgcaaatcg
     1261 cggtcgacgc caacggaacg atc
//
LOCUS       DASUCJ010000464         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_860828, whole genome shotgun sequence.
ACCESSION   DASUCJ010000464 DASUCJ010000000
VERSION     DASUCJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860828"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>1039
                     /locus_tag="VFU90_05565"
     CDS             163..>1039
                     /locus_tag="VFU90_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5479278.1"
                     /translation="MRTAEPLVMAAAPDAVLNALPHPVIVVAADGRVAHANVAAEAFF
                     EMSLPLLRRHSLGDLVPFGSPLLVLVDQVRGSGAAVNEYKVDLGTPRNPGDRSVDLHV
                     APLPEQPDYVVVMLQERSIADKMDRQLTHRGAARSVIALAAMLAHEIKNPLSGIRGAA
                     QLLEQSVGDEDRTLTRLICDEADRIVKLVDRMEVFSDERPVEREPVNIHVVLDHVKRL
                     AQSGVARHVKFVEDYDPSLPPVLANRDQLVQIFFNLVKNAAESIADSPGGEIALSTAF
                     RPGVRLSIPGSKTRIS"
BASE COUNT          173 a          346 c          318 g          202 t
ORIGIN      
        1 ctctatgcag aatgaaggcg tcgcctaatt cgtgtgcata gcgttcttat gctccctttt
       61 tccgatcagg ttgcctccag gtcaagcgtc tttcctggat cggagccctc ggaatgcacg
      121 cgatccggcg caagcagcaa ggatggttgc gaggatggtc gtatgagaac cgccgagcca
      181 ttggtcatgg ccgcggctcc cgacgcggta ctcaatgcgc tgccgcatcc agtcattgtc
      241 gtcgctgccg acggacgcgt cgcgcacgcc aacgtggcgg ccgaagcctt tttcgaaatg
      301 tcgctgccgc tgctgcggcg ccattcgctg ggcgacctcg tgcccttcgg cagtcctctg
      361 ctcgttctcg tcgatcaggt ccgcggcagc ggagccgcgg tcaacgaata caaggtcgat
      421 cttggaacgc cgcgcaatcc cggcgaccgc tcggtcgatt tgcacgtcgc gccgctgccc
      481 gagcagccgg actacgtggt cgtcatgctg caggagcgtt cgattgccga caagatggac
      541 cggcagctca cccatcgcgg cgccgcgcgt tcggtcatcg cgcttgccgc gatgctcgcc
      601 cacgaaatca agaacccgct gtccggaatt cgcggcgcgg cgcaactgct cgagcaatcc
      661 gtcggcgacg aagaccgcac tctgacgcgg ctgatctgcg acgaggccga tcgcatcgtc
      721 aagctcgtcg atcgcatgga agtcttctcc gacgagcgcc cggtcgagcg cgagccggtc
      781 aacattcacg tcgtgctcga tcacgtaaag cggctcgccc agtcgggcgt cgcccgccac
      841 gtgaaattcg tcgaggacta cgatccgtcg ctgccgccgg ttctggcgaa ccgcgatcag
      901 cttgtgcaga tctttttcaa tctggtgaaa aacgccgccg agtcgatcgc cgacagcccg
      961 ggcggcgaga tcgcgctgtc cacggccttt cgtcccggcg tgcggctctc gattccgggc
     1021 tcgaagacgc gcatttcgc
//
LOCUS       DASUCJ010000465         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_769234, whole genome shotgun sequence.
ACCESSION   DASUCJ010000465 DASUCJ010000000
VERSION     DASUCJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769234"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFU90_05570"
     CDS             <1..854
                     /locus_tag="VFU90_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU5479279.1"
                     /translation="AMLVGLLVLWGCMLIAGYGAIFWGLRGGLTPAVHSLGVATYFAG
                     TSMTTIGFGDVAGKTLGTRFFSILAGATGLGLFSIITAYLFALFGSFQTRESFVVMLG
                     ARTGTPPSGVDLLAIAGYSSTLGDLDVLLLEAQRWTAQIMESHLAYPVLAYFRSSHDY
                     QSWVGTLGTLLDASTLLMTTVEGVPSGEARIFYNLGRHATHDLARHLAILPENVTPGL
                     ERGEFETACNRLARAGYRLHDRDAAWERFATMRSTYAPHINALARFFEIPPLQWIGDR
                     GPVEGPH"
     gene            complement(872..>1286)
                     /locus_tag="VFU90_05575"
     CDS             complement(872..>1286)
                     /locus_tag="VFU90_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5479280.1"
                     /translation="APAKVLAANADLDVALLRTSRRDLPVVRLGTSAHLQSEVGRRIG
                     LLGYPVPDEFDDESLGLATSLNAGLLSSVRKDALEVTMSIVPGESGAPVFFADSGEVI
                     GIAESRFDEEHSIGFALPIDDATKFLHRVDRAHGF"
BASE COUNT          192 a          456 c          408 g          230 t
ORIGIN      
        1 cggcgatgct cgtcggcctg ctggtactgt ggggatgcat gctgatcgcg ggctacggcg
       61 cgattttttg gggtttgcgc ggcgggctca cgccggcggt gcattcgctc ggcgtcgcaa
      121 cctactttgc cggcacctcg atgaccacga tcgggttcgg cgacgtcgcc ggaaaaacgc
      181 tgggaacgcg cttcttctcg atcctcgccg gcgccaccgg gctcgggctc ttttcgatca
      241 tcaccgcgta cttgttcgcg ctcttcggtt cgtttcaaac gcgcgagtcg ttcgtcgtga
      301 tgctcggcgc gcgcaccggc acgccgccca gcggcgtcga tttgctggcg attgcgggct
      361 actcgtcgac cctcggcgat ctcgacgtgc tgctgctcga agcccagcgg tggacggcgc
      421 agatcatgga gagccatctc gcctatcccg tgctcgccta cttccgttcc agccacgact
      481 atcagtcgtg ggtgggaacc ctcggcacgc tgctcgacgc ttcgacgctg ctgatgacca
      541 ccgtcgaagg cgtacccagc ggcgaagcgc gcatcttcta caatctgggg cggcacgcga
      601 cccacgattt agcgcgccac ctcgccatct tgcccgagaa cgtcacgccc gggctcgagc
      661 gcggcgaatt cgaaacggcc tgcaaccggc tcgcgcgcgc cggctaccgc cttcacgacc
      721 gcgacgccgc gtgggaacgt ttcgcgacga tgcgctccac ctacgccccg cacatcaacg
      781 cgctcgcgcg ctttttcgag attccgccgc tgcagtggat cggcgaccgc ggccccgtcg
      841 agggcccgca ctagccgccg ccgcgcgcgc cctaaaagcc gtgcgctctg tcgacgcggt
      901 ggagaaactt cgtcgcgtcg tcgatcggca gtgcgaaacc gatgctgtgc tcttcgtcga
      961 agcgcgattc cgcaatgccg atcacctcgc cgctgtcggc aaagaacacc ggcgctccgc
     1021 tttcgccggg gacgatcgac attgtgactt cgagcgcgtc cttgcgcacc gacgacagca
     1081 gtccggcgtt gagcgacgtg gccagcccga gactttcgtc gtcgaactcg tccggcacgg
     1141 gatagccgag cagaccgatg cggcgcccga cctcggactg caagtgggcc gacgtcccga
     1201 gccgcaccac cggtaaatcc cgccgtgacg tccgcagcag cgcgacgtcg aggtcggcgt
     1261 tggccgcgag caccttggcc ggcgcg
//
LOCUS       DASUCJ010000466         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_539604, whole genome shotgun sequence.
ACCESSION   DASUCJ010000466 DASUCJ010000000
VERSION     DASUCJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539604"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..944)
                     /locus_tag="VFU90_05580"
     CDS             complement(114..944)
                     /locus_tag="VFU90_05580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5479281.1"
                     /translation="MPSLYRAYVKDSSARGLMGSAMRRPDPLETALEAAAEAGAALLA
                     YLAGPREIAEKGRRADLVTDADRASEAIVLARLRAAYPDATIVAEESGTHAGTSDERW
                     IVDPLDGTTNFAHRYPLFCISIAYERAGELIAGVVYAPAMDEWFAAERGAGARLNART
                     IGASNVATLAGAMTCTGFHPADFERNAACFRAVSQRAQAVRRDGSAALDLAYVACGRF
                     DGFWEFDLNPWDVAAGTLLVREAGGTVTRADGSPASLDARSILATNGRIHAELAAALE
                     "
     regulatory      947..1143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            1148..>1574
                     /locus_tag="VFU90_05585"
     CDS             1148..>1574
                     /locus_tag="VFU90_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5479282.1"
                     /translation="MAFTEGFISELVGRRATVNDLSIGKVADFLVSKPEDTFPQIDGL
                     VIKTSRGLRFAPIDTVADVDRNGTVALTMAPKEPAPSNEEELYLIADLLDKQIVDVDG
                     RKVVRINDIEVADAGGHLRVVGADVGVAGLLRRLGLKSFG"
BASE COUNT          264 a          527 c          534 g          249 t
ORIGIN      
        1 cgcaaaagcc aggatggcgt attcctttgg tgttgcgaaa aagcgcccga gcaggagcga
       61 gcacggacgc gagcgacgag cgtccgagcg acggcatccc cgccatcgcg cgttcattcc
      121 agggcggcgg cgagttcggc gtggatgcgc ccgttggtgg cgaggatcga acgtgcgtcg
      181 agcgacgccg gcgagccgtc ggcgcgcgtg acggtgccgc cggcttcgcg caccagcagc
      241 gttccggcgg cgacgtccca cggattgagg tcgaactccc agaagccgtc gaagcggccg
      301 cacgccacgt aggcgagatc gagcgcggcc gacccgtcgc gccgcaccgc ctgcgcgcgt
      361 tgcgagaccg cgcggaagca cgccgcgttg cgctcgaaat ccgccggatg aaagcccgtg
      421 caggtcatcg cccccgccag cgtcgcgacg ttggacgcgc cgatcgttcg ggcgttcagc
      481 cgggcgcccg ccccgcgctc cgcggcgaac cactcgtcca tcgccggggc gtacacgacg
      541 cccgcgatca gctcgcccgc gcgttcgtac gcaatcgaaa tgcagaacag cggatagcga
      601 tgtgcgaaat tcgtcgtacc gtcgagcgga tcgacgatcc agcgctcgtc gctggttccg
      661 gcgtgcgtcc cgctttcttc ggcaacgatc gtcgcgtccg gatacgccgc ccgcagccgc
      721 gccagcacga tggcttcgct cgcgcggtcg gcgtcggtca cgaggtcggc gcggcgccct
      781 ttttcggcga tttctcgcgg gccggcgagg tacgcgagca acgccgcgcc cgcttccgca
      841 gctgcttcca gcgccgtttc gagcgggtcg gggcgtcgca tcgccgagcc cattagaccg
      901 cgcgctgagg agtccttgac gtaggcgcgg tataatgaag gcatggacta gacctcgcta
      961 ggtgaggcgt ccacgcattt acacgccgct gcccggaaac gtcgagagac gccaagtggg
     1021 tagaccagac tacgccgaga caaggcgatg tctaacgcgg ctgagcctct gtgggccgtg
     1081 cgcccaggag agtcggggct cgacggtgcg tggtgccaaa gctcgctgcg aatggggtac
     1141 gagagacatg gcgttcacgg aagggttcat ctccgaactg gtcggccgcc gcgcgaccgt
     1201 caacgatctt tccattggaa aggtcgccga ctttttggtc agtaagcccg aagacacgtt
     1261 tccgcaaatc gacggcttgg tcatcaagac ctcgcgcggt ctgcgcttcg cgcccatcga
     1321 cacggtcgcc gacgtcgatc gcaacggcac cgtcgcgctg acgatggcgc ccaaagagcc
     1381 ggcgccgagc aacgaagaag agctctatct catcgccgat ctgctggaca agcagattgt
     1441 cgacgtcgac ggccgtaagg tcgttcgcat caacgatatc gaagtcgccg acgccggcgg
     1501 ccacctgcgc gtcgtcggcg ccgacgtcgg cgtcgccggt ctcttgcggc gccttgggct
     1561 caagtcgttc gggc
//
LOCUS       DASUCJ010000467         1122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_708117, whole genome shotgun sequence.
ACCESSION   DASUCJ010000467 DASUCJ010000000
VERSION     DASUCJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708117"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1122
                     /locus_tag="VFU90_05590"
     CDS             <1..>1122
                     /locus_tag="VFU90_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU5479283.1"
                     /translation="DDFAAPANPSSARLALGALFLGITLAIAGGTIGVALRHPPLSLN
                     EIVRAVAERLVGVRTISLPSRIDHLVTPALLAVGIGIALAFCWLLFRPAVAARASTYR
                     PLSRERARFLVERYGTDSLSYFALRGDKEWFGFRDTLVAYRVHNGIALVSPDPVGPVG
                     QRSEAWGAFREFADEHGWSVAVMGASAEWLPAYRASGMHDLYIGDEAVVDVRRFSLEG
                     KQNKSLRQSVGRVEKAGYRVEFFDPAKIEPALRDELRGLMSESRKGDVERGFSMTLGR
                     AFEAEDKGLLLAVAFDGDNHAAGFCQYVPARSIQGWSLDLMRRSETRDVPNGLTEFIV
                     TSTIAHLRERGDVGLALNFATFRAVLASEAGDRLVHRAQ"
BASE COUNT          164 a          361 c          382 g          215 t
ORIGIN      
        1 gcgacgactt cgcggcgccg gccaacccgt cctcggcacg tctcgcattg ggcgcgctgt
       61 tcctcggcat cacacttgcg atcgccggcg gaacgatcgg tgtcgcgttg cgtcacccgc
      121 cgttgtcgct caacgagatc gtccgtgctg tcgcggaacg cctcgtcggc gtgcgcacga
      181 tctcgttgcc gagccggatc gaccacctcg tgacgccggc tttgctcgct gtcggcattg
      241 gcatcgcgct ggcgttctgc tggctgttgt tccggcctgc cgtcgccgcg cgggcgagca
      301 cgtaccgtcc gttgtcgagg gaacgcgccc gcttcctcgt cgagcgttac ggcaccgatt
      361 ccctttcgta cttcgcgttg cgcggcgaca aagaatggtt cgggttccgc gacacgctgg
      421 tggcgtaccg agtgcacaac ggaattgcct tggtttcacc ggacccggta ggtccggtcg
      481 gtcaacgttc cgaggcatgg ggcgcgttcc gcgagttcgc cgacgagcat ggctggtcgg
      541 ttgcggtcat gggcgcgtcc gccgaatggc tcccggcgta tcgcgcaagc ggcatgcacg
      601 acctgtacat cggcgacgag gcggtcgtcg acgttcgacg cttctcgctc gagggcaagc
      661 agaacaagag ccttcggcag tcggtcgggc gcgtcgagaa ggccggctac cgcgtcgaat
      721 tcttcgatcc cgcgaagatc gagccggcgt tgcgtgacga gctgcgcggc ttgatgtccg
      781 agagccggaa aggcgacgtc gagcgcgggt tctcgatgac gctcggtcgc gcattcgaag
      841 ccgaggacaa gggattgctg ctcgcggtgg cgttcgacgg cgacaaccac gcggcaggct
      901 tttgtcagta cgttcccgcg cgctcgatcc agggctggtc gctcgatctc atgcgccgtt
      961 cggagacacg cgacgttccg aacgggttga ccgagttcat cgtgacctcg acgatcgcgc
     1021 acctgcgcga gcgcggtgac gtcggtcttg cactgaactt cgcgacgttc cgagcggtgc
     1081 tcgcaagcga agcaggcgac cgactcgtgc atcgcgcgca ga
//
LOCUS       DASUCJ010000468         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_876251, whole genome shotgun sequence.
ACCESSION   DASUCJ010000468 DASUCJ010000000
VERSION     DASUCJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876251"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFU90_05595"
     CDS             complement(<1..300)
                     /locus_tag="VFU90_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479284.1"
                     /translation="MQAAVLAVDPGRSKAGFALVAADGGVIESGIVAVGELQGRLAAL
                     LAAYPVEALALGRGTNAAALASQLGGLGLRLTLVDEFETSRRARELYFLEHPPRGW"
     gene            complement(306..1247)
                     /locus_tag="VFU90_05600"
     CDS             complement(306..1247)
                     /locus_tag="VFU90_05600"
                     /inference="COORDINATES: protein motif:HMM:NF022727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HEU5479285.1"
                     /translation="MNFVEIVRGAGNVGIVLLIAGAVAYVGDRVGHQVGRRRLSLFGI
                     RPRYTSTIIAIGTGMVIALVVTLVAIFASQQVKTAFFKLNSINQQIGELTRREQDLET
                     KVNTGRLVEPVDVLMVPFYRIVDQSASPKTRLATIRAFYFDSVKFINANYPRLGLKPY
                     AIPPDVDKKLEALDLMIAAKQSLGNVMLTATSDQNLFQNDQIHFGINATADVRVYQKG
                     QAIAQLTIPGKSGANANIALGQLENYVAIDARRLGMPPFLADNVQPLQLIPDVHQMQQ
                     RISRSGTYVLTAFAAEDFYPHNARIPIVIVLTQQPEG"
     gene            complement(1267..>2122)
                     /locus_tag="VFU90_05605"
     CDS             complement(1267..>2122)
                     /locus_tag="VFU90_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU5479286.1"
                     /translation="DSELTAMKAAGITFSRIVAPLLAVGIVMSFVTLYLQEELVPFAN
                     DQVVEIEDNAINHTSAYNRDLTVSAPLPGGGRQVTIATAYEPNSRALLHVTLIQYDNR
                     NQPRQIVFADRAEFAVDKWTLQNSSLYRFDPDGSVLAQPRIPEQQVEIGENPTDLVKR
                     IKNDDPDSMSRAEIAGVVRSGQLTESELRKYVTTYQEKLARPFACFVFILIAVPFGLR
                     SVRSGGSSASLGFGLSLAIVFVYYVVMTICSYLGEAFLSVATLWAWMPNLIFTAIGLS
                     RLRRAATV"
BASE COUNT          379 a          690 c          663 g          390 t
ORIGIN      
        1 ccaaccgcgc ggcggatgtt ccaagaagta gagttcgcgc gcccggcggc tcgtctcgaa
       61 ctcgtcgacc agcgtcaacc ggaggcccaa accgccaagc tgcgacgcca gggcggcggc
      121 gttggtcccg cggcccagcg cgagggcttc gacggggtac gccgccagca gcgccgcgag
      181 ccgtccctgg agttcgccga ccgcgacgat cccggactcg attacgccgc cgtcggcggc
      241 taccagcgcg aagccggcct tgctccggcc cgggtcgact gccagcaccg ccgcctgcac
      301 gcaggtcagc cttccggctg ctgggtcaat acgatcacga tcgggatgcg cgcgttgtgc
      361 ggatagaaat cctcggccgc aaacgccgtc aacacgtagg ttcccgacct cgaaatgcgc
      421 tgctgcatct gatggacgtc gggaatcagc tgcagcggct gcacgttgtc ggcgaggaac
      481 ggcggcattc ccagccgccg cgcgtcgatt gccacgtaat tctccagctg cccgagcgcg
      541 atgttcgcgt tcgcaccgct cttgccggga atcgtgagct gagcgatcgc ctggcccttt
      601 tgatacaccc gcacgtcggc ggtggcgttg attccaaagt ggatctggtc gttttgaaag
      661 aggttctgat ccgacgttgc ggtcagcatc acgttcccaa gcgactgctt cgcggcaatc
      721 atcaaatcga gggcttcgag cttcttgtcg acgtcgggcg gaatcgcgta cggcttgagc
      781 ccgaggcgcg ggtagttggc gttgatgaac ttcaccgagt cgaagtagaa cgcacgaatc
      841 gtcgcgagcc gcgtcttcgg cgacgccgat tggtcgacga tccggtaaaa cggtaccatc
      901 agcacgtcca ccggctcgac cagccgcccc gtgttcacct tggtttcgag gtcctgctcg
      961 cggcgcgtca gctcgccgat ctgctggttg atcgagttca gcttgaagaa cgccgtcttt
     1021 acctgctgcg aagcgaaaat cgccaccagc gtcaccacca gcgcgatcac catccccgtg
     1081 ccgatcgcga tgatcgtcga cgtgtagcgc gggcggatgc cgaacagcga cagccgccgc
     1141 cgccccactt gatgaccgac ccggtcgccg acgtaggcta cagcgccggc gatcagcagc
     1201 acgatgccga cgtttccggc gccgcgaacg atttcgacga aattcattcg ccggcttccg
     1261 cggccttcac acggtcgctg cccggcgcag ccgcgagagc ccgatcgccg taaagatgag
     1321 gttcggcatc cacgcccaga gggttgcgac gctcaagaac gcttcgccga gatacgaaca
     1381 gatcgtcatc acgacgtagt acacgaacac gatcgcgagc gacaagccga agccgaggct
     1441 cgcactgctg ccgccgctgc gcaccgagcg caagccgaac ggcaccgcga tcagaatgaa
     1501 aacgaagcac gcaaatgggc gcgcgagctt ttcttgatac gtcgtgacgt acttgcgcag
     1561 ttcgctctcc gtcaactgac ccgagcgcac gaccccggcg atctcggccc ggctcatgct
     1621 gtcgggatcg tcgtttttta ttcgcttgac cagatcggtt ggattttcgc cgatttcgac
     1681 ctgctgctcc gggattcgcg gctgggctag caccgaaccg tcggggtcga accggtagag
     1741 gctggaattt tgcagggtcc atttatcgac ggcgaactcg gcccgatccg cgaacacgat
     1801 ttgccgcggc tgattgcggt tgtcgtactg aatcagcgtc acgtgcagca gcgcccgcga
     1861 gttgggctcg tacgcggtcg cgatcgttac ctgccggccg ccccccggga gcggcgccga
     1921 tacggtgagg tcgcggttgt aggcgctggt gtggttgatc gcgttgtcct cgatttccac
     1981 cacctggtcg ttggcgaacg gcaccagctc ctcttgaagg tagagggtga cgaacgacat
     2041 tacgatcccg accgccagca gcggggcgac gattcgcgaa aacgttattc cggcggcctt
     2101 catcgcggtg agttcactgt cg
//
LOCUS       DASUCJ010000469         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_769265, whole genome shotgun sequence.
ACCESSION   DASUCJ010000469 DASUCJ010000000
VERSION     DASUCJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769265"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFU90_05610"
     CDS             <1..229
                     /locus_tag="VFU90_05610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479287.1"
                     /translation="AFFVHPAFARRGLARGLYAECVGAARAAGFRRIELMATLPGEPV
                     YRALGFSEIERIEATLPGGVVIPLVRMARAV"
     gene            344..814
                     /locus_tag="VFU90_05615"
     CDS             344..814
                     /locus_tag="VFU90_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479288.1"
                     /translation="MLPHVLGVIALIAVALLFGATWYETVVMAPNYERDIPASIALAR
                     QFLAGATPARFFRVVAPAAQLLTLGAVVFDWAKPGDNAFLVALIALIVADVITYTFHY
                     PRLAVMFTSPEPQDPAVLRRAARQWATGNWVRGILLFVVFIALLAGTVHLARHL"
     gene            840..1157
                     /locus_tag="VFU90_05620"
     CDS             840..1157
                     /locus_tag="VFU90_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5479289.1"
                     /translation="MIGRTWHGRVPAKKADAYHKYLLRTGLSDYAATPGNRGVFVLRR
                     DDGDVTHFLLLTLWESLDAIRAFAGEAYDRARYYPEDDDYLLEREPFVVHYDVLAASG
                     VKE"
BASE COUNT          166 a          423 c          381 g          218 t
ORIGIN      
        1 cgcgttcttc gtgcacccgg cgttcgctcg acgcggcctg gcgcgaggac tctatgccga
       61 atgcgtcggc gcggcgcgcg cggcgggatt ccgtcggatc gagctcatgg ccacgctccc
      121 cggtgaaccc gtgtatcgcg cactgggctt ctcggagatt gagcgcatcg aggccacact
      181 gccgggcggc gtcgtgatcc cgctcgtgcg catggcgcgc gctgtgtgac cccgacatcc
      241 gcatttgcgc agggcaaggg accggtgcca cattcagcgg cgaccgtttc cggcgtcgtg
      301 catcgccgcc gcgccgtcct cgttgcctaa cgcaaaccca tcgatgcttc cccacgtcct
      361 cggtgtcatc gcgctcatcg ccgtcgcgct gctcttcggc gcgacgtggt acgagaccgt
      421 cgtcatggcg cccaactacg agcgggacat tcccgcgtcc atcgcactcg cgcggcagtt
      481 tctggcgggc gccacgccgg cgcggttctt ccgcgtcgtc gcccctgccg cacagctgct
      541 cacgctcggc gccgtggtgt tcgactgggc caagcccggc gacaacgcat ttctcgtcgc
      601 cctcatcgcg ctgatcgtgg ccgacgtgat cacgtacacg ttccactacc cgagactcgc
      661 tgtgatgttc acgtcgccgg agccgcagga tccggcggtg ttgcgccgcg cggctcgcca
      721 gtgggcgacg ggcaactggg tgcgcggcat cttgctgttc gtcgtgttca tcgcgctgct
      781 cgccggcacg gtgcatctcg cccggcacct ctaagcctaa cgctagccat cgagggccca
      841 tgattggacg aacctggcac ggccgcgtgc cggccaagaa agccgacgcc tatcacaagt
      901 atctgctgcg tacgggactc tccgactacg ccgcgacccc cggcaaccgc ggcgtattcg
      961 tgctgcgccg cgacgacggt gatgtgacgc acttcttgct gctcacactc tgggagtcgc
     1021 tcgacgcgat ccgcgcgttc gccggcgagg cctacgaccg ggcgcgctac tacccggagg
     1081 acgacgatta tctgttggaa cgcgagccgt tcgtcgtgca ctatgacgtg ctcgcggcct
     1141 cgggcgtgaa ggaatgagcc ccgaaacgcg tcgcaccgcg ggcttgta
//
LOCUS       DASUCJ010000470         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1151080, whole genome shotgun sequence.
ACCESSION   DASUCJ010000470 DASUCJ010000000
VERSION     DASUCJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151080"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /gene="uraH"
                     /locus_tag="VFU90_05625"
     CDS             <1..356
                     /gene="uraH"
                     /locus_tag="VFU90_05625"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU5479290.1"
                     /translation="MGRLSTHVLDTVRGGPADGVRIDLVVADGTDWRTLKSVTTNDDG
                     RTGEPVLEGDALKPGRYELVFHVADYFARRGTAQGTPPFLDTVPIRFTIADPDAHYHV
                     PLLVTPWSYSTYRGS"
     gene            375..1580
                     /locus_tag="VFU90_05630"
     CDS             375..1580
                     /locus_tag="VFU90_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU5479291.1"
                     /translation="MEAYVVDWLNMLVRWLHLIAGIAWIGASFYFVMLDNSLMPPKKE
                     EDRNHGVFGELWAVHGGGFYNSRKYLTGPKGEPLSEHLHWSKWEAYTTWISGLGLLAI
                     IYWHGASAFLIDTGMMNLTPTAAIAISIGFLVGGWLVYDALCRILEGQEMLLGLVLFV
                     LIVIADWGLFHLFSARAAYIHVGAMIGTMMVANVFFHIIPGQKRMVAAIRAGKEPDPR
                     PGVIGKQRSVHNTYFTLPVLFIMISNHYPMTYGNPQGWVVLAAIMLAGVLIRQFFVLR
                     HRGRVNYALPAAGAVILAAVAIAIAPQPTPARASVGDAKVAFSEVRPIIAERCAVCHS
                     EKPTQPGFAAPPAGVLLDSPDHIHDNAQRIEQQAVATQTMPLANLTKMTPAERALIGR
                     WIAEGAKTH"
     gene            1594..2955
                     /locus_tag="VFU90_05635"
     CDS             1594..2955
                     /locus_tag="VFU90_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HEU5479292.1"
                     /translation="MSKSTHPVDEVLPFGQMLAVGIQHVLVMYAGAIAVPLLMGGVLK
                     MPKDQVAFLISSDLFSCGIVTLMQCVGIWKFGIRLPAIMGVSFAPVGAMIAIANGGAG
                     IQGILGAVIGAGIFGVLIAPVFGKLMRFFPPVVTGSVITIIGVTLFPVGINWAAGGFG
                     SKTFGDPRFIGISLVVLVVILLINKFLRGFVANISVLLGMVAGFIIALALGFVDFTGV
                     GAAAWFAPVRPFAFGAPTFDAAAIASLCLVMIVIMVESLGMFLALGDITGRPANHDSV
                     TRGLRADGLATVFAGIFNTFPHTSFSQNIGLVSITGVRSRWVVVVSGVILFMLGLLPK
                     ISTIVASIPQAVLGGAGIAMFGMVAATGIKILSNVNYESKNNLLIVAVSFGMGMIPLV
                     SPSFFQHFPAWSSPITHSGITLTAITAILLNALFNRAESIEEVERELAADATFPLDSP
                     DKA"
     gene            3112..3936
                     /locus_tag="VFU90_05640"
     CDS             3112..3936
                     /locus_tag="VFU90_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479293.1"
                     /translation="MKRITVGACAVALIATTAAMQQAAAEEWSDTSIGWRYGTQFKEP
                     YVANGAAISKNIIDLQHVGGYKYGTNFFNVDFLMSDSTDSNAQEAYVTYRNTIDFGKV
                     TGQKYQFGPVRSVGMTLGFDWNTKNDPGYGSKKRMLVIGPTLMFDVPGFLDASVLVLH
                     ESNKPVGVDSRYTYRTHPMLSLAWGIPFGSTGLAFEGYFNWIAAKGTNEFGGPTAPET
                     NFDGEVMYDVASLVHLPKNALRVGFEYQYWRNKFGNPSDVPGSLAKTPMVRVEYHF"
     gene            4163..>4471
                     /locus_tag="VFU90_05645"
     CDS             4163..>4471
                     /locus_tag="VFU90_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479294.1"
                     /translation="MKPEPTPTSSAPGRTAPGFLVLLVASGTIISLCMGLRQSLGLFM
                     RPMTLDLGVSAATFGFSIALQNIVWGLSQPVVGALADRHGPRPVLIATSIVYAAGLAL
                     M"
BASE COUNT          739 a         1519 c         1437 g          776 t
ORIGIN      
        1 cgatgggacg gctctcgact cacgtgctcg acaccgtgcg cggaggcccg gccgacggcg
       61 tccgcatcga cctcgtcgtc gcggacggga cggactggcg cacgctgaag tcggtcacca
      121 ccaacgacga cgggcgcacc ggcgagccgg tcctcgaagg cgacgcgctg aagcccggac
      181 gctacgagct cgtgttccac gtggccgact acttcgcccg acgcggcacc gcgcagggca
      241 cgccgccgtt cctcgacacg gtgccgatcc gcttcacgat cgccgatccg gacgcccact
      301 accacgtgcc gctgctcgtc acgccgtgga gctactcgac ctaccgcgga agctgagcga
      361 cacggaggcc cccgatggaa gcgtacgtcg tcgactggtt gaacatgctg gtgcgctggc
      421 tccatctgat cgccggcatc gcgtggatcg gcgcgtcgtt ctacttcgtg atgctcgaca
      481 actcgctgat gcctccgaag aaggaagaag accggaacca cggcgtgttc ggcgagcttt
      541 gggccgtgca cggcggcggt ttctacaaca gtcggaagta cctgaccggg ccgaagggcg
      601 agccgctctc cgagcacctg cactggtcga agtgggaggc ctacacgacg tggatatcgg
      661 ggctcgggct cctcgcgatc atctactggc acggtgcgag cgcgttcctg atcgacacgg
      721 gcatgatgaa cctgacgccc actgcggcga tcgcgatcag catcggcttc ctcgtcggcg
      781 gctggctcgt ctacgacgcg ctttgccgca tcctggaagg ccaggagatg ctgctcgggc
      841 tcgtcctgtt cgtgctcatc gtcatcgccg actggggcct ctttcacctc ttcagtgcac
      901 gcgccgcgta catccacgtg ggcgcgatga tcggcacgat gatggtggcg aacgtgttct
      961 tccatatcat ccccgggcag aaacgaatgg tcgccgcgat ccgcgccggc aaggagcccg
     1021 atccgcgccc tggcgtcatc ggcaagcagc gctcggtgca caacacctat ttcacgctgc
     1081 cggtgctgtt catcatgatc agcaaccact atccgatgac ttacggcaac ccgcagggct
     1141 gggtcgtcct cgcggcgatc atgctggccg gcgtgctgat ccggcagttc ttcgtgctgc
     1201 gccaccgcgg cagggtgaat tacgcgctgc cggctgcggg tgcggtgatc ctcgccgccg
     1261 tcgccatcgc gatcgcgccg cagccgaccc cggcccgcgc gagcgtcggc gacgcgaagg
     1321 tcgcgttcag cgaggtgcgg ccgatcatcg ccgagcgctg cgccgtctgc cactcggaga
     1381 aaccgacgca gccgggcttc gcagccccgc cggcaggcgt gctcctcgat tctcccgacc
     1441 acatccacga caacgcgcag cgcatcgagc agcaggccgt ggcgacgcag acgatgccgc
     1501 tcgcgaacct cacgaagatg acacctgccg agcgcgcgct catcggcagg tggatcgccg
     1561 aaggcgcgaa gacccattga ccaggagagg cccatgagca aatcgaccca ccccgtcgac
     1621 gaggtcctgc cgttcggcca aatgcttgcg gtaggcatcc agcatgtgct cgtcatgtac
     1681 gccggcgcga tcgcggtgcc gctcctcatg ggcggcgtgc tgaagatgcc caaggatcag
     1741 gtcgcgttcc tgatcagctc cgatctgttt tcctgcggca tcgtcacgct gatgcagtgc
     1801 gtcggcatct ggaagttcgg catccggctg ccggcgatca tgggtgtgag cttcgccccg
     1861 gtcggcgcga tgatcgcgat cgccaacggc ggcgccggga tccagggcat cctcggcgcc
     1921 gtcatcggag ccggcatctt cggcgtgctg atcgcgccgg tgttcggcaa gctgatgcgc
     1981 ttcttcccgc cggtggtcac cggatcggtg atcacgatca tcggcgtcac gctgttccct
     2041 gtcgggatca actgggccgc cggaggattc ggcagcaaga ccttcggcga tccgcgcttc
     2101 atcggcatca gcctcgtcgt gctggtcgtg atcctgctga tcaacaagtt cctgcgcggc
     2161 ttcgtcgcca acatctcggt gctgctgggg atggtggcgg gcttcatcat cgcgctcgcg
     2221 ctcggcttcg tcgacttcac cggcgtcggc gccgcagcct ggttcgcgcc ggtgcgcccg
     2281 ttcgccttcg gtgcaccgac cttcgacgcc gccgcgatcg cctcgctctg cctcgtgatg
     2341 atcgtcatca tggtcgaatc gctcggcatg ttcctggcgc tcggcgacat caccggtcgc
     2401 ccggccaacc acgacagtgt cacgcgcggg ctgcgcgccg acggcctggc aacggtgttc
     2461 gcgggcatct tcaacacgtt cccgcacacg tcgttctcgc agaacatcgg cctggtcagc
     2521 atcaccggtg tcagaagtcg ctgggtggtc gtggtttccg gcgtgatcct gttcatgctc
     2581 gggctgctgc cgaagatctc gacgatcgtc gcctcgatcc cgcaggcggt gctgggcggc
     2641 gcgggcatcg cgatgttcgg catggtcgcc gcgaccggaa tcaagatcct gtcgaacgtc
     2701 aattacgaga gcaagaacaa tctgctgatc gtcgccgtga gcttcggcat ggggatgatt
     2761 ccgctcgtgt cgccgagctt cttccagcac tttcctgcgt ggagcagccc gatcacgcac
     2821 agcggcatca cgctgaccgc gatcacggca atcctgctca acgcgctgtt caaccgcgcc
     2881 gagtcgatcg aggaagtcga gcgcgagctc gcggcagacg ccactttccc gctcgacagc
     2941 cccgacaagg cgtaacgcgc gtcaacacca tgtgcgcgag cgctaacggc agccgtgggc
     3001 cgccggaacg atgaagttgc cgcgcgcgcc cggccgcgac cggaccgcac ggagtggtgc
     3061 tgcaccccgc aatccagaca cttttattcc atccaatcgg agaacatcga gatgaagagg
     3121 attaccgtcg gagcgtgcgc cgttgcactg atcgcaacga cagccgcgat gcagcaggcc
     3181 gccgccgagg agtggagcga cacgtcgatc ggctggcgct acggcaccca gttcaaggag
     3241 ccctacgtcg ccaatggcgc cgcaatctcc aagaacatca tcgacctgca gcacgtcggc
     3301 ggctacaagt acggcaccaa ctttttcaac gtcgacttcc tgatgtccga cagcaccgac
     3361 agcaatgcgc aggaggcgta tgtcacttac cgaaatacga tcgacttcgg caaggtgacc
     3421 ggacagaagt accagttcgg gccggttcgc agcgtcggca tgacgctcgg cttcgactgg
     3481 aacaccaaga acgatcccgg ctatggttcg aagaagcgga tgctggtgat cggcccgacg
     3541 ctgatgttcg acgtccccgg ctttctcgat gcgagcgtgc tggtgctgca cgagagcaac
     3601 aagccggtcg gggtcgacag ccgctacacg tacaggaccc acccgatgct gagcctcgcc
     3661 tggggcatcc cgttcggctc gaccgggctg gccttcgagg gctatttcaa ctggatcgcc
     3721 gcgaagggga ccaacgagtt cggcggcccg acagcgccgg agacgaattt cgacggtgaa
     3781 gtcatgtacg acgtcgcgtc gctcgtgcac ctgccgaaga acgcgctgcg cgtcggcttc
     3841 gagtaccagt actggcgcaa caagttcggc aatccttccg atgtcccggg ctcgctcgcg
     3901 aagaccccga tggtgcgtgt cgagtaccac ttctgaccca cccctccggc acggcggcgg
     3961 gccgcgcgca tgagcgcgcc gacccgccgg cgacgctatg gccggccctg cgaggccgcc
     4021 gcgccgatgc gccgcggcga gtccatcgaa cgaacgcgca tgagcgaggc gcatgcgccg
     4081 cgtgagcaaa gagcattggg cgatcgcgag cccggtgcct taagatgtcg cagccgctcc
     4141 ctgcggcaac gcgcgagcgc tagtgaagcc cgaacccacc ccgacgtcct ccgcgcccgg
     4201 ccggaccgcg cccggctttc tcgtcctgct cgtcgcgagc ggcacgatca tctcgctgtg
     4261 catggggctg cggcagagcc tcggattgtt catgcgaccg atgacgctcg acctcggcgt
     4321 gtcggcggcg acgttcggct tctcgatcgc gctgcagaac atcgtctggg ggctgtcgca
     4381 gccggtcgtc ggcgcgctcg ccgaccgcca cgggccgcgg ccggtgctga tcgcgacgtc
     4441 gatcgtgtat gccgcggggc tcgcgctgat g
//
LOCUS       DASUCJ010000471         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1258344, whole genome shotgun sequence.
ACCESSION   DASUCJ010000471 DASUCJ010000000
VERSION     DASUCJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258344"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>1384
                     /locus_tag="VFU90_05650"
     CDS             48..>1384
                     /locus_tag="VFU90_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5479295.1"
                     /translation="MNDHVQNEKTRVAAISVFASAGMAVAKLVVGIVIGSLALISEAL
                     HSSVDVVATIITWMVVRVSDRPADDEHHYGHGKFESISALGVIALLYVLAGGILVESY
                     SRLREGVAPPTISAIPFVVLLVDIAVNFWRARALQRTARETQSQALAADALHFASDVL
                     GSIAVIIGLALAGLGFRWGDSAAAIAVAIMIAALGLRMARSTVETLLDRAPEGVSEKA
                     RTAIEQVPGVVAVERVRVRMVGATHFIDAIVKVPRTYPIDRVDEIKRKAQGAVSAALG
                     DADLTFTVAPVALDNESVRERIMVIARNSGLAVHHVTVHDLGGRLIVSIDLEVDGDMA
                     LTAAHDITRGLERSIRDEFGAEVEVDTHIEPLEPELPFGIDAPPDRVETIRTALTRFA
                     ADTPIHDIHNVRVRDTDAGEIVNFHCRAAASMSVIKVHETVDEIERALRRAFPT"
BASE COUNT          220 a          462 c          447 g          255 t
ORIGIN      
        1 tgatcccatg ctttcttgtc cctgccgggg ctgctaagca ggcgccgatg aacgatcacg
       61 tgcagaacga aaagacccgc gtcgcggcga tctcggtctt tgccagcgcc ggcatggccg
      121 tggccaagct cgtcgtcggc atcgtcatcg gctcgctcgc gctgatttcc gaggcgctgc
      181 actcttcggt cgacgtggtc gcgaccatca ttacctggat ggtggtccgt gtctccgatc
      241 gccccgccga cgacgagcat cattacggtc acggcaagtt cgaaagcatc tccgcgcttg
      301 gcgtgatcgc gctgttgtac gtgctggcgg gcggcatcct cgtggaatcc tacagccggc
      361 tgcgcgaagg cgtagccccg ccgaccatct cggcaatccc gtttgtcgtg ctcctggtcg
      421 acattgccgt caatttctgg cgcgcccgcg cgctacagcg cactgcgcgt gagacccaaa
      481 gccaggcgct ggcggccgac gcgctgcatt tcgcctcgga cgtgctgggc tcgattgccg
      541 tcattatcgg gctcgcgctc gcgggcctcg gtttccgatg gggcgattcg gccgccgcta
      601 tcgcggttgc gatcatgatc gcggctctgg gtttgcggat ggcgcgttcc acggtggaga
      661 ccctgctcga ccgcgcgccc gaaggcgtct cggaaaaggc cagaaccgcg atcgagcagg
      721 tcccgggcgt cgtcgccgtc gaacgggtgc gggtgcgcat ggtcggcgcc acccatttca
      781 tcgacgcgat cgtgaaggtg cctcgcacct atccgatcga ccgggtcgac gagatcaagc
      841 ggaaggcgca aggcgcggtc agtgccgcgc tcggcgatgc cgacctgacc ttcaccgtcg
      901 ctcccgtggc gctcgacaat gagagcgtcc gcgagcggat catggtgatc gcccgcaatt
      961 ccggcctcgc cgttcatcac gtcaccgtgc acgatctcgg cggcaggctg atcgtgagca
     1021 tcgatctcga ggtcgacggg gatatggcgc tgacggccgc tcacgacatc acccgaggtc
     1081 tggagcgcag catccgcgac gagttcggcg ccgaggtcga ggtcgacacg catatcgagc
     1141 cgctggaacc ggaattgccg ttcggcatcg acgccccgcc cgaccgcgtc gagaccatca
     1201 ggacggcgtt gacgcgcttt gccgccgaca cccccatcca cgacatccat aatgtgcgag
     1261 tgcgcgacac cgacgccggc gagatcgtga actttcattg ccgcgccgcg gcatcgatga
     1321 gcgtgatcaa ggttcacgag actgtcgacg agatcgagcg cgcgttgcgt cgcgcctttc
     1381 cgac
//
LOCUS       DASUCJ010000472         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_800058, whole genome shotgun sequence.
ACCESSION   DASUCJ010000472 DASUCJ010000000
VERSION     DASUCJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800058"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFU90_05655"
     CDS             <1..314
                     /locus_tag="VFU90_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5479296.1"
                     /translation="FRTIRDLGTVSGSASRPRNRLRSTFRSLGMLIGLAAGEFLSDAE
                     QLRGDALDDLRRRADTLGANAVVGLQFFVSENSDGACKVVATGRAVFVDKASAGETDD
                     A"
     gene            304..984
                     /locus_tag="VFU90_05660"
     CDS             304..984
                     /locus_tag="VFU90_05660"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5479297.1"
                     /translation="MTLEERARRERLLAQAAAAANACRRCAIGSERRNNVYGEGDSLA
                     RLMVVGEGPGETEDLLGRPFVGRAGQLLDRMLAAIGFAREEVYICNTVKCRPTLPGPR
                     GPRNRAPEPQEMANCRPFLDEQIDIIAPQVILALGAPAAKSFLGSDFQITKMRGRWYT
                     GPNGIDLMVSFHPAYVLRQTGGEIHAVKRLVWADLKAVRAKLDELAARPPERPPEPEQ
                     RGLFGDAL"
     gene            1009..>1412
                     /locus_tag="VFU90_05665"
     CDS             1009..>1412
                     /locus_tag="VFU90_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479298.1"
                     /translation="MNREPRALWLQHVAGRAGFDGVEAFEREYGLKPYRLRQLYRAAT
                     KELVPDVEAVTPLPKELRAALTARGVAFSSVDPIVERRSSDGQTTKALFRLHDGKEVE
                     AVLMEHAHGRTTVCISSQAGCAFACAFCSTGQA"
BASE COUNT          224 a          446 c          505 g          237 t
ORIGIN      
        1 gcttccggac gatccgcgat ctgggaaccg tttcgggcag cgcgtcgcgg ccgcgcaacc
       61 gcttgcgctc gacctttcgc agcttaggga tgctgatcgg gttggcggcc ggcgaatttt
      121 tgagcgacgc cgagcagctg cgcggcgacg cgctcgacga tttgcgccgg cgcgccgata
      181 cgctgggcgc caacgccgtg gtcgggctgc agtttttcgt gagtgaaaat tcggacggcg
      241 cgtgcaaggt ggttgccacc ggacgcgcgg tcttcgtgga taaagcttcg gcgggcgaaa
      301 ccgatgacgc ttgaagagcg ggcgcgccgc gagcggctgc tcgcgcaagc ggccgccgcc
      361 gccaacgcct gccggcggtg tgcgatcggt tcggaacggc gcaacaacgt gtacggcgag
      421 ggcgactcgt tagcgcggct gatggtggtc ggcgagggcc cgggagaaac cgaggatttg
      481 ctcgggaggc cgttcgtcgg gcgggcgggc caattgctcg atcggatgct cgcggcgatc
      541 gggttcgcgc gcgaagaagt ctacatctgc aacaccgtga agtgccggcc gacgctcccg
      601 gggccgcgcg gtccgcgcaa tcgagccccc gagccgcagg agatggcgaa ctgccgtccg
      661 ttcctcgacg agcagatcga catcatcgcg ccgcaggtga ttctggcgct cggcgctccg
      721 gctgcgaagt cttttctcgg cagcgatttt caaatcacca agatgcgcgg tcgctggtat
      781 accggtccga acgggatcga tctgatggtt tcgttccatc ccgcttacgt gctgcggcag
      841 acgggcggag agatccatgc ggtcaaacgg ctggtctggg cggatctgaa ggcggtgcgc
      901 gccaagctcg acgaactcgc ggcgcgtccg ccggaacggc cgccggaacc cgagcagcgc
      961 gggctattcg gcgatgcgct ctagagcacg cgtgctacaa tgggccgcgt gaaccgggaa
     1021 cctcgcgcgc tctggctgca gcacgtcgcc ggccgagccg gcttcgacgg cgtcgaggcg
     1081 ttcgagcgcg agtacggcct caagccgtac cgtctccgcc aactctatcg cgcggcgacc
     1141 aaggaacttg tgccggacgt cgaggccgtc acgccgctgc ccaaagagct gcgcgccgcg
     1201 ctgaccgcgc gcggtgtcgc cttctcgtcg gtcgatccga tcgtggaacg ccgttcgagc
     1261 gacgggcaga ccaccaaagc gctttttcgg cttcacgacg gcaaggaggt cgaagcggtg
     1321 ctgatggaac acgcgcacgg gcggacgacg gtttgcatct cgtcgcaagc cggatgcgcg
     1381 tttgcatgcg ctttttgctc gaccggccag gc
//
LOCUS       DASUCJ010000473         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1319402, whole genome shotgun sequence.
ACCESSION   DASUCJ010000473 DASUCJ010000000
VERSION     DASUCJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319402"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..>1063)
                     /locus_tag="VFU90_05670"
     CDS             complement(88..>1063)
                     /locus_tag="VFU90_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5479299.1"
                     /translation="HISMTGALINLPAVLIVAAISYVCYVGVTQSASLNAVVVAIKLV
                     VILLFVAFSLQFVNPANWHPFVPPEDASGHFGWPGVFRAASIVFFSYIGFDAVSTAAQ
                     EAKNPQKDMPIGILGSLVVCTILYIIVSAVLTGITPYTMLGTAHPVSTAIEGYPSLRW
                     LRIVIEVGAIAGLSSVILVMLMGQPRIFYTMAKDGLMPPSFGKVHPKYQTPYVGQAIV
                     GVIAAILAGLLPINVLGDLVSMGTLLAFTTVCIGVLVLRRTRPDLPRGFRVPAAPVVC
                     VLGALSCFALFFIAFMDNWLWMSAWIVIGLLVYFMYGYRRSKLRGAQR"
BASE COUNT          217 a          328 c          366 g          152 t
ORIGIN      
        1 accagtagtt ggtcgagcat ggtcatgtcg cgtcggcagt cgtgcggcta gaaaaaagcc
       61 ggcgggcatg ccgccggctt tcgggtttca gcgctgtgcg ccgcgcagct tgctgcgtcg
      121 gtagccgtac atgaagtaga cgagcaggcc gatgacgatc caggccgaca tccacagcca
      181 gttgtccatg aacgcgatga agaacagcgc gaagcagctg agcgcgccga gcacgcagac
      241 gaccggcgcc gccggcacgc ggaatccgcg cggcaggtcc ggacgcgtcc ggcgaagcac
      301 gagcacgccg atgcagacgg tcgtgaacgc gagcagcgtg cccatcgaga cgagatcgcc
      361 gagcacgttg atcggcagca gtcccgcgag gatcgcggcg atgacgccga cgatcgcctg
      421 gccgacgtac ggcgtctggt acttcggatg caccttgccg aacgacggcg gcatcaggcc
      481 gtccttcgcc atcgtgtaga agatgcgcgg ctggcccatc agcatgacga ggatgaccga
      541 cgagaggccc gcgatcgcgc cgacctcgat cacgatgcga agccagcgca ggctgggata
      601 accctcgatc gccgtcgata ccgggtgcgc ggttccgagc atcgtgtacg gcgtgattcc
      661 agtcaggacg gccgagacga tgatgtagag gatcgtgcag acgaccagcg agccgaggat
      721 gccgatcggc atgtccttct gcggattctt cgcttcctgc gccgcggtcg agaccgcgtc
      781 gaagccgatg tacgagaaga acacgatcga cgccgcgcgg aacacgccgg gccagccgaa
      841 gtgcccgctc gcgtcttcgg gcggcacgaa cggatgccag ttggccgggt tcacgaactg
      901 cagcgagaac gcgacgaaga ggaggatcac gacgagcttg atcgcgacga cgaccgcatt
      961 cagcgacgcc gattgcgtga cgccgacata gcagacgtag ctgatggccg cgacgatcag
     1021 caccgccggc aggttgatca gcgcgccggt catcgagatg tgg
//
LOCUS       DASUCJ010000474         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1365385, whole genome shotgun sequence.
ACCESSION   DASUCJ010000474 DASUCJ010000000
VERSION     DASUCJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365385"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFU90_05675"
     CDS             complement(<1..1197)
                     /locus_tag="VFU90_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5479300.1"
                     /translation="MTVDATPTRGVETLDEVVIRFAGDSGDGMQLVGDRFTELSAAFG
                     NDLATLPNFPAEIRAPAGTIAGVSSFQVHISDHDIVTPGDTPNVLVAMNPAALRANIA
                     DLVPGCTLLVNVDTFDTRNLDKAGYAQNPLRDGSLAAYRVFEIPMTSVTIEATKPLGV
                     KPRDAERSKNFFALGLICWMYTRPTEPLFDWIKKRFANRPEVMEANLAAFRAGYNFGE
                     TAELFDHPFEVLPARLAPGEYRQITGNVALAYGLVAAAQQAKLPLFYASYPITPASDI
                     LHELSKLKNFGVKTLQAEDEISAAGIAIGAAFAGNLAVTGTSGPGVDLKSEALGLAVS
                     LELPMIIVDVQRGGPSTGLPTKTEQADLMLAMYGRHGEAPMPIIAASSPSDCFAAAFE
                     AAPLAIK"
BASE COUNT          203 a          429 c          430 g          206 t
ORIGIN      
        1 cttgatcgca agaggtgccg cttcgaacgc ggcggcgaag cagtcgctcg gcgagctcgc
       61 ggcgatgatc ggcatcggcg cctcgccgtg ccgcccgtac atcgcgagca tgaggtcggc
      121 ttgctcggtc ttcgtgggca ggccggtcga cgggccgccg cgctgcacgt cgacgatgat
      181 catcggcaac tcgaggctca ccgcgagccc gagcgcctcc gacttcaggt cgacgccggg
      241 accgctcgtg ccggtcaccg cgaggttgcc ggcgaacgcc gcgccgatcg cgatgccggc
      301 cgcggagatc tcgtcttcgg cttgcaaggt cttcacaccg aagttcttca acttcgacag
      361 ctcgtgcagg atgtcggacg ccggcgtgat ggggtacgac gcgtagaaca acggaagctt
      421 cgcctgctgc gcggccgcga cgagcccgta ggcgagcgca acgttgcccg tgatctggcg
      481 gtactcaccg ggcgcgagcc gcgcgggcag cacttcgaac gggtggtcga acaactccgc
      541 ggtctcgccg aagttgtacc ccgcgcggaa cgccgcgagg ttggcctcca tgacctccgg
      601 ccggttcgcg aaacgcttct tgatccagtc gaacagcggc tcggtcggac gcgtgtacat
      661 ccagcagatc agcccgagcg cgaagaagtt cttcgaacgc tcggcgtcac gcggcttcac
      721 accgagcggc ttcgtcgctt cgatcgtcac cgacgtcatc gggatctcga acacgcggta
      781 cgcggcgagc gaaccgtcgc gcaacgggtt ctgcgcgtag cccgccttgt cgagattgcg
      841 cgtgtcgaac gtgtcgacgt tcaccagaag cgtgcagccc ggcacgaggt cggcgatgtt
      901 cgcccgcaac gccgcggggt tcatcgcgac gaggacgttc ggcgtgtcgc cgggcgtgac
      961 gatgtcgtgg tccgagatgt gcacctggaa cgacgacacg cccgcgatcg tcccggcggg
     1021 cgcgcggatc tcggcgggga aattcggcag cgttgccaag tcgttgccga acgcggcaga
     1081 cagctcggtg aagcgatcgc ccaccagttg catgccgtcg cccgagtcgc ccgcgaaccg
     1141 gatcacgacc tcgtcgagtg tctccacgcc gcgggtcggc gtcgcgtcta cggtcaccgt
     1201 tgaccaccag ctttctcagt acctgtggcg acgggcgaca ccacgccatc gggatggcgc
     1261 cccacgcg
//
LOCUS       DASUCJ010000475         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_708227, whole genome shotgun sequence.
ACCESSION   DASUCJ010000475 DASUCJ010000000
VERSION     DASUCJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708227"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFU90_05680"
     CDS             complement(<1..183)
                     /locus_tag="VFU90_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479301.1"
                     /translation="MPENEHEYVARLTWDGNTGEGTASYERYGRGYRVHIAGKSDLAG
                     SADPMFRGDGRLHNPED"
     gene            complement(278..>1140)
                     /locus_tag="VFU90_05685"
     CDS             complement(278..>1140)
                     /locus_tag="VFU90_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479302.1"
                     /translation="GMLLDIHDPVNPKRLYAASDSNFSYWPSATFNNDGTKMLFSDEW
                     GGGGQPKCRATDPKDWGADAIFTITNNALHFQSYYKMPAPQTEMENCVAHHGSLIPIP
                     GRDVMVQSWYQGGISVFDWTDAAHPKEIAFFDRGPIDSTRMMLGGTWSAYWYNGVIVS
                     SEIVRGLDILELTPSAYLSQNEIDAAKTVHLDYLNVQGQPKFVWPPSFALARAYVDQL
                     ERSNGLSGSRISSVRKSLTEAEQASGSSRQTGLTQLASQLDGDSGRSSDGAKVQTLAG
                     AVRALAGASQ"
BASE COUNT          194 a          393 c          349 g          204 t
ORIGIN      
        1 gtcctccgga ttgtggagcc ggccgtcgcc gcgaaacatc ggatcggcgg aacccgcgag
       61 atcggatttg cccgcgatgt gtacgcggta gcctcgcccg taccgctcgt agctcgcggt
      121 gccctcaccg gtgttcccgt cccacgtgag ccgggccacg tactcgtgct cgttctccgg
      181 catcgcccct cccgcgttgc agcgtgaatg cggcgagaaa cgacgagggg catccgcgat
      241 cgggatgccc ctcatcgcta tgcctaacgg tagtgactca ctgcgacgcg cccgcgagcg
      301 cgcgaaccgc gccggccagc gtctgcacct tcgcgccgtc cgacgagcgg ccggaatcgc
      361 catccaactg cgacgccagc tgcgtgagtc ccgtctgccg cgacgagccg gatgcctgct
      421 ccgcctcggt caacgacttc cgaacggaag agatgcgcga ccccgacagc ccgttggacc
      481 gctcgagctg atcgacgtac gcgcgcgcca gcgcgaagct cggcggccac acgaacttgg
      541 gctgcccctg cacgttcaga tagtccaggt gcaccgtctt cgccgcgtcg atctcgttct
      601 gcgataaata cgcgctcggc gtcaactcca ggatgtcgag accgcgcacg atctccgagc
      661 tcacgatgac gccgttgtac cagtacgcgg accacgtccc gcccaacatc atgcgcgtcg
      721 agtcgatcgg tccgcggtcg aagaacgcaa tctccttcgg gtgcgcggcg tcggtccaat
      781 cgaacaccga gatgccgccc tgataccacg actgcaccat cacgtcgcgt ccgggaatcg
      841 gaatgagcga gccgtggtgc gccacgcaat tctccatctc cgtctgcggc gccggcatct
      901 tgtagtaact ctggaagtgc agcgcgttgt tcgtgatcgt gaagatcgcg tcggcgcccc
      961 aatctttggg atccgtcgcg cggcacttgg gctgaccgcc gccgccccac tcgtccgaga
     1021 acaacatctt ggtgccgtcg ttgttgaacg tcgcggaggg ccagtacgag aagttcgaat
     1081 cgcttgcggc gtagagacgt ttgggattca cggggtcatg gatgtccaac agcatcccgt
//
LOCUS       DASUCJ010000476         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_325763, whole genome shotgun sequence.
ACCESSION   DASUCJ010000476 DASUCJ010000000
VERSION     DASUCJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325763"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VFU90_05690"
     CDS             complement(<1..1216)
                     /locus_tag="VFU90_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479303.1"
                     /translation="MALGAFVACGGPSSAPTGPRDDAGSNAAPESDAGGESDAASRTN
                     AGSAADASAGNGGPTGTDASKGGDFATGDASSGADGGDGGVPSKATVSSWLGTNVAAD
                     LPRVDVTYQLTPFDTPSAQLDANGYPIAGASGTSSTDIGFVLPSGTYNIEYTGAGALT
                     VSGIGRLGGAWQSAGGEQRNTLAITGTPGQFGNFLKLTIANGAGQTVQGIHIYYPGFD
                     FDSPTTFLPQFISLLGPFRAMRFMDWESTNNSTLANWADRPAAAHFGQSSFGEPYEHI
                     AALVNETGKDCWITVPELATTDFITQFASFMASNLDFGRIQSARDRAGFTEPFQIIVE
                     NSNETWNQGFSAYGTFLAVANADTARYTGAYSGSYGPSWMTQSVDLLKVAEVEADRLV
                     TIGNVFRSAFGAV"
BASE COUNT          220 a          426 c          455 g          245 t
ORIGIN      
        1 cgaccgcgcc gaatgccgag cggaagacgt tgccgattgt gactaagcga tcggcttcga
       61 cctcggccac cttcaagagg tccaccgatt gtgtcatcca cgacgggccg tagctgccgc
      121 tgtacgctcc cgtgtaccgc gccgtatcgg cgttcgcgac ggcgaggaac gtcccgtacg
      181 ccgagaatcc ctggttccac gtctcgttgc tgttctcgac gatgatctgg aacggctcgg
      241 tgaatccggc gcggtcgcgc gcggactgga tgcgtccgaa atcgaggttc gacgccatga
      301 agctcgcgaa ctgcgtgatg aagtccgtcg tcgccagttc tggcacggta atccagcagt
      361 ctttgccggt ttcgttgacg agcgcggcga tgtgctcgta cggctcgccg aagctcgact
      421 gaccgaagtg tgccgcggcg gggcggtcgg cccagttggc gagggtgctg ttgttggtgg
      481 actcccagtc catgaaccgc atcgcgcgga acggaccgag aagcgaaatg aactgcggga
      541 ggaacgtcgt cggggaatcg aaatcgaatc ccggataata aatgtggata ccctggactg
      601 tctgtcccgc gccgttcgcg atggtgagct tcaggaaatt gccgaattga cccggggtcc
      661 ccgtgatcgc cagcgtgttt cgctgctcgc cgccggccga ctgccaggcc ccgccgagtc
      721 ttccgattcc ggataccgtg agtgcgccgg cgcccgtgta ttcgatgttg taggtcccgc
      781 tcggaagaac gaagccgatg tccgtcgagc tcgtgcccga agccccggcg atgggatagc
      841 cgttcgcgtc cagctgagcc gaaggcgtgt cgaagggcgt cagctggtag gtgacgtcga
      901 cgcgcggcag atcggcggcg acattggtcc cgagccacga agagaccgtg gctttcgagg
      961 ggacgccgcc gtcgccgcca tcggccccgc tgctcgcatc gcccgtcgcg aagtcgccgc
     1021 ccttcgatgc atcggtgccc gtagggcccc cgttacccgc cgaagcatcg gcggcgctgc
     1081 ccgcgttcgt ccgggacgcc gcgtcgcttt cgccgccggc atccgattcc ggcgcggcgt
     1141 tcgatccggc gtcgtcgcgt ggcccggtgg gcgccgagga tggtccgccg caggcgacga
     1201 atgcgcccag agccactgcg agcagccaat gcgcggggca gctgtctttc gggtgcatgc
     1261 gaggacctcc gcgaaagagg attgcccgcc ttaagcgaga ttgcatgtcg aacattgcat
     1321 cgacgcaatc gctggtgcac gaaatg
//
LOCUS       DASUCJ010000477         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1426378, whole genome shotgun sequence.
ACCESSION   DASUCJ010000477 DASUCJ010000000
VERSION     DASUCJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426378"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFU90_05695"
     CDS             complement(<1..868)
                     /locus_tag="VFU90_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479304.1"
                     /translation="MKLKGILALAASLVAATAFAQVKVGVITSATGPTAFVGIPQKNA
                     VALLPKKAGDLTIEYIVYDDASDPTQSVQLVRKLLSEDRIDALIGPSGSPNAMGVLQF
                     MAEAKTPMLAPVGTSAVVVPMDERKHWVFKTHPNDDVIAEGLIAAMTKRGVKTLGFIG
                     YNDPYGESWYKTFAPMAQKAGINIVANERYARTDTSVTGQVAKLIAARPDAVLVAGVA
                     AGAALPHMQLVDAGYKGAIYHTHGSASGAFVQVGGRKVEGALIVGPMLLVLGEIPDSV
                     SSKRVALEFVSSY"
     gene            complement(899..>1188)
                     /locus_tag="VFU90_05700"
     CDS             complement(899..>1188)
                     /locus_tag="VFU90_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479305.1"
                     /translation="RVHALASRKPSIASAPVAIAPLAPIEPQSASGQTLSREVLAILE
                     DSVHRAAAAPTLAQALDIGYRAFGASACTAAACEGIQANREGRKPDFGTTG"
BASE COUNT          173 a          417 c          381 g          217 t
ORIGIN      
        1 cgtagctcga cacgaactcg agcgcgaccc tcttcgacga cacgctgtcg gggatctcgc
       61 ccagcaccag cagcatcggc ccgacgatca gcgcgccctc gaccttcctg ccgccgacct
      121 gcacgaacgc tccggaggcc gagccgtgcg tgtgataaat cgcacccttg tagcctgcat
      181 cgacgagctg catgtgcggc agcgccgctc ccgccgcgac gccggcaacc agtaccgcgt
      241 cgggcctggc cgcgatcagc ttcgccacct gtccggtcac gctggtgtcg gtgcgtgcgt
      301 agcgctcgtt ggcgacgatg ttgatgccgg ccttctgcgc catcggcgcg aaggtcttgt
      361 accagctctc gccgtacgga tcgttgtagc cgatgaagcc gagcgtcttg acgccgcgct
      421 tcgtcatcgc cgcgatcaat ccctcggcga tcacgtcgtc gttcgggtgg gtcttgaaca
      481 cccagtgctt ccgttcgtcc atcggcacga ccaccgccga cgtgccgacc ggtgccagca
      541 tcggcgtctt cgcctcggcc atgaactgca gcacgcccat cgcgttgggc gagccgctgg
      601 gcccgatcag tgcgtcgatc ctgtcttcgg acagcagctt cctgacgagc tgcacgctct
      661 gcgtcgggtc gctcgcatcg tcgtagacaa tgtattcgat cgtgaggtca cccgccttct
      721 tcggcagcag cgccaccgcg ttcttctgcg gaatgccgac gaacgcggtc gggccagtgg
      781 ccgacgtgat cacgccgacc ttcacctgcg cgaaggccgt tgcggcaacg agcgacgccg
      841 cgagtgcgag gatgcccttg agcttcatct gtctttctcc tgggagtgaa tcgatggtct
      901 agccggtcgt gccgaaatcg ggcttgcgtc cttcccgatt cgcctggatg ccttcgcacg
      961 ccgccgcggt gcaggcggag gcgccgaacg cgcggtagcc gatgtcgagc gcttgcgcga
     1021 gcgttggcgc cgcagccgcc cggtgcaccg agtcttcgag gatcgccagc acctcgcggc
     1081 tcagcgtctg cccgctggct gactgcggct cgatcggcgc caggggagcg atcgcaaccg
     1141 gcgcggaagc gatcgatggc ttgcgcgacg ccagcgcatg cacgcgtg
//
LOCUS       DASUCJ010000478         1027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_769409, whole genome shotgun sequence.
ACCESSION   DASUCJ010000478 DASUCJ010000000
VERSION     DASUCJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769409"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFU90_05705"
     CDS             <1..470
                     /locus_tag="VFU90_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479306.1"
                     /translation="LTPAIGSEMAITFTLPKNTVGAELPSEGVGSFTDPTWGKIGGYT
                     QSTTAQVLAFPPGVTITFTNLSSTHPHTLNVVKKVLGPPAHFPLNPSLPTQPMGNGIL
                     GKGFASGSVSPGKSVSIKLLNKGNYLIGCAFHYHEGMQDVIRVNAHATPGPTH"
     gene            471..>1027
                     /locus_tag="VFU90_05710"
     CDS             471..>1027
                     /locus_tag="VFU90_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5479307.1"
                     /translation="MTLRSALAAFAAAAALALQPSVGAAVQSAPQLVDQTGRTFTFES
                     LRGRPLIVTFVSAHCTDACPLVNAQFADAAERIAQRHLSTRLLTITLDPANDPPSLMR
                     ALARRFAADPRYWLVASGRPADVDAVLNAFGVVARLGKSGYREEHTTFVYVLDSSGKL
                     SKTMLASTGLTGDIVDAARSLQPRTG"
BASE COUNT          187 a          356 c          305 g          179 t
ORIGIN      
        1 ggctcacccc tgccatcggc tcggaaatgg ccatcacgtt cacgctgccg aaaaataccg
       61 tcggcgcgga acttccatcg gaaggtgtgg gcagcttcac cgatcctacg tggggaaaaa
      121 tcggcggata cacgcaatcg acgaccgcac aagttctagc tttcccgccg ggcgttacca
      181 ttacgtttac caacctgtcg tcgacgcatc cgcacaccct caacgtcgtc aagaaggtct
      241 tggggccgcc ggcgcacttc ccgctgaacc catcgttgcc gacgcagcca atgggtaacg
      301 gcattctcgg aaagggtttc gccagcggca gcgtctcgcc gggcaagtcg gtcagcatca
      361 agctgctcaa caaaggcaac tatctgatcg ggtgcgcgtt ccactaccac gaagggatgc
      421 aagacgtcat tcgcgtcaac gcgcacgcta cgccggggcc cacgcactaa atgaccctgc
      481 gatccgcttt ggcggccttc gcggccgcgg ccgctctcgc actgcagccc tccgtaggcg
      541 ctgcggtgca gagcgcgccg cagctcgtcg atcaaaccgg ccgcaccttc acgttcgaat
      601 cgctgcgcgg acgtccgttg atcgtgacgt tcgtgtcggc ccactgcacc gacgcgtgtc
      661 cgctggtcaa cgcgcagttc gcggatgccg ccgaacggat cgcacaacgg cacctctcca
      721 cgcggctgct cacgatcacc ctcgaccccg ccaacgatcc gccgagcttg atgcgggcgc
      781 tcgcacggcg gttcgcggcg gatccgcgct attggctggt cgcgagcgga cggcccgccg
      841 acgtcgacgc cgtattgaac gctttcggcg tggtcgcacg cttgggcaag agcggctatc
      901 gcgaggagca cacgaccttc gtctacgttc tggattcgtc cggtaagctc agcaaaacta
      961 tgctggcgtc gaccggactc accggtgaca tcgtcgacgc cgcgcgctcg ctgcagccga
     1021 ggaccgg
//
LOCUS       DASUCJ010000479         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1487530, whole genome shotgun sequence.
ACCESSION   DASUCJ010000479 DASUCJ010000000
VERSION     DASUCJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487530"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1214)
                     /locus_tag="VFU90_05715"
     CDS             complement(192..1214)
                     /locus_tag="VFU90_05715"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479308.1"
                     /translation="MLIAHLRLGLLLAMLIASLGVAAEPRVWTLTDVQVQRFSPNALS
                     NPTDPVTGWFVYDDATRTITNWSVRFREPYFDFPSFTYVPGNSLTYVALLGGPIDPPF
                     LGFSAVLGEGPDFAVRQLQLVPLGALDGSNAVVALDTSESRVDYVLLGPTTRRSIATG
                     SLTLAPQPPPVVIAQVDEFYHAVLRHYYITADEAEKQALDAGVYPGWQRTGESFRAYA
                     AASRTGGSINPVCQFYSPPIIEGGDELDVVGADSHFFSADAAECLAVFRKWWWFWGFP
                     QDNVFQIDVPDRKSGACAVGTIPVYRLWNQRVDSNHRYTTSAAIRAQTLAAGYLDEGV
                     RMCALQ"
BASE COUNT          249 a          445 c          488 g          234 t
ORIGIN      
        1 catgtgtcgc tgctggtgcg cgtcgccggc gacgccagtg gatcattcgc ctggacgcgc
       61 gtcggggggc tgctcaatgc gctcgcgctc gcggcgttcg tgctcggcac ggcgagcgcg
      121 gccgtgcgcg ggaagcatgc cgcggcgcgc gcagcctgat cgccaagggt cttgccgcga
      181 tttctggctg gttactgcaa tgcgcacatc cgcacgcctt cgtcgagata gccggcagcg
      241 agcgtctgcg ccctgatcgc tgcactcgtc gtgtagcggt ggttggaatc gacgcgctga
      301 ttccacaggc ggtacacggg aatggttccg accgcacatg cccccgattt cctgtcgggc
      361 acgtcgatct gaaacacgtt gtcctggggg aaaccccaaa accaccacca cttccggaac
      421 accgccaggc actcagccgc gtccgccgaa aagaagtgcg aatcggcgcc cacgacgtcc
      481 agctcgtcgc cgccctcgat gatcggaggg ctgtagaact ggcacaccgg gtttatggag
      541 ccaccggttc tgctggcggc ggcgtacgcc ctgaatgact ccccggtgcg ttgccagccc
      601 ggatagacgc cggcgtcgag ggcctgtttc tcggcctcgt cggcggtgat gtagtagtgg
      661 cgcaagaccg cgtgatagaa ctcgtcgacc tgggcgatga cgaccggcgg tggctgcggc
      721 gcaagcgtga gcgagccggt tgcgatcgac cggcgggtcg tcggtccgag gagaacgtaa
      781 tcgactctcg attcggaagt gtcgagcgca acgacagcgt tgctgccgtc cagcgctccg
      841 agcggcacga gttgcagttg ccggaccgcg aaatccggtc cttcgcccag cacagcggag
      901 aaacccagga acggcggatc gattggaccg ccgagaagcg cgacgtacgt cagagagttg
      961 cccggaacgt aggtgaagct cggaaaatcg aaatacggct cgcgaaatcg cacgctccag
     1021 ttcgtgatcg tccgcgtagc atcgtcgtag acgaaccagc cggtgacggg gtccgtcggg
     1081 ttggaaaggg cgttggggct gaacctctga acctgcacgt cggtgagagt ccaaacccgc
     1141 ggctcggcgg cgacgccaag cgaagctatg agcattgcaa gcaggaggcc gaggcgaagg
     1201 tgtgcgatca gcatgatgag cctccctgcg agttcgatgc gcgggcggcc ctggatccgc
     1261 tgcggccgcg cactcgcgct acgcctcgtg aacgtaggcg acaagtgggc cgaccttacg
     1321 gcagcgcgcg taggagttgt tcggccggca gccgcgcgct ttcaggcgcc gcaacgcccg
     1381 ggccagactt ccgccccggt cagcgatgca gctgtg
//
LOCUS       DASUCJ010000480         1381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_402240, whole genome shotgun sequence.
ACCESSION   DASUCJ010000480 DASUCJ010000000
VERSION     DASUCJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402240"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFU90_05720"
     CDS             complement(<1..228)
                     /locus_tag="VFU90_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5479309.1"
                     /translation="MHEPVARNSTRAVFRARGLAKDYVAGTVVVRALHDVDLDLFEGE
                     FVVILGPSGSGKSTLLNILGGLDVPTAGDVVF"
     gene            complement(244..945)
                     /locus_tag="VFU90_05725"
     CDS             complement(244..945)
                     /locus_tag="VFU90_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5479310.1"
                     /translation="MQTNRTRVLIVDDEPQILEVLQAYLEREGLAVECSGSVNGALEA
                     VERARPDLMVLDVTLPDGSGLDVLRKTAIPGERIPTIMLTARSAEADRVVGLELGADD
                     YVTKPFSPREVVARVRALLRRIAEGSVTPKPHKTTRIGELEIDHEFHEVRIGGRPASL
                     TATEFRILALLAENPGEVFTRSHMLDRLKDGGEIYERTLDRHINNLRKKIEVDASRPE
                     YVVTVYGVGYKMRRP"
     gene            complement(949..>1381)
                     /locus_tag="VFU90_05730"
     CDS             complement(949..>1381)
                     /locus_tag="VFU90_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479311.1"
                     /translation="PALAVFQTRLWFFGMLIMSFSMHFAGLLGAPRRTAEVAYMGAAA
                     ANSWHPLMILAAAGGFFLFASILAFVAVAIGTLVQNEKTHSMDASFATPANPADATPA
                     ALQHLYRWGLVALAFAVLAYIGPLGELLRHPGYLAPGMRTW"
BASE COUNT          237 a          458 c          467 g          219 t
ORIGIN      
        1 aaagacgacg tcgccggcgg tcgggacgtc gagcccgccg aggatgttca gcagagtcga
       61 tttcccgctt cccgacgggc ctagaatgac gacgaactct ccttcgaaca agtcgagatc
      121 gacgtcgtgg agcgcgcgaa cgacgaccgt gccggcgaca taatccttgg ccaggccgcg
      181 cgctcggaaa acggcccgcg tcgaattccg cgctaccggc tcgtgcatgg gcctacggcc
      241 gcctcagggg cgccgcattt tatagccgac gccgtagacg gtcacgacgt attcggggcg
      301 gctggcgtcg acttcgattt tcttgcgcag gttgttgatg tgccggtcga gcgtgcgttc
      361 gtagatttcg ccgccgtcct tcaggcggtc gagcatgtgc gagcgcgtga acacctcgcc
      421 ggggttttcg gccagcagcg cgaggatgcg aaactcggtg gccgtgaggc tggcgggcct
      481 tccgccgatg cgcacctcgt gaaactcgtg atcgatctcg agctccccga tgcgggtcgt
      541 cttatgcggt ttgggcgtga ccgagccttc ggcgatccgg cgcagcaacg cgcgcacccg
      601 ggccacgact tcgcgcggtg agaacggctt ggtgacgtag tcgtcggcgc cgagctccaa
      661 gccgaccacc cggtcggctt ccgccgaacg cgccgtcagc atgatcgtcg gaatccgctc
      721 gccgggaatc gcggtcttgc gcagcacgtc gaggccgctg ccgtcgggca aggtgacgtc
      781 gagcaccatc aaatcgggcc gcgcgcgctc gaccgcctcg agcgcaccgt taacgctgcc
      841 ggagcactcg acggccagcc cttcacgctc gagataggct tggagcacct cgaggatctg
      901 cggctcgtcg tcgacgatca gcacgcgcgt ccggttcgtc tgcatgcgtc accaggttcg
      961 catccccggc gcgagatacc ctggatggcg cagcagctcg ccgagcggtc cgatgtaggc
     1021 cagcaccgcg aaggccagcg cgaccagccc ccaccgatac aggtgctgca gggccgccgg
     1081 cgtcgcatcg gccgggttgg ccggggtggc gaacgaggcg tccatcgagt gagtcttctc
     1141 gttctgcacg agcgtgccga tggcgacggc cacgaacgcg aggatgctgg cgaacaggaa
     1201 gaatccgccc gccgccgcga ggatcatcag cggatgccac gagttggcgg ccgcggcgcc
     1261 catgtacgcg acctcggcgg tacggcgcgg cgcgcccagc aatccggcga agtgcatcga
     1321 gaacgacatg atcagcatac cgaaaaacca cagccgcgtt tgaaaaacgg ccagcgccgg
     1381 c
//
LOCUS       DASUCJ010000481         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1212780, whole genome shotgun sequence.
ACCESSION   DASUCJ010000481 DASUCJ010000000
VERSION     DASUCJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212780"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFU90_05735"
     CDS             complement(<1..527)
                     /locus_tag="VFU90_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479312.1"
                     /translation="MYRPDPLDVRLADALPPGSLYAVGGRVRDELRAATENIQPSGKD
                     LDYLVTGVPIEELRRRLSGLGRVDVVGAAFAILKLGIDGRTVDVGLPRRERSVGHGHR
                     DFEIQSGPDVPLEEDLARRDFRMNMLARALPGGALIDPYGGEADIRARRIDVLRPEAF
                     EEDPLRMLRAAQFAAR"
     gene            complement(940..1614)
                     /gene="rsmG"
                     /locus_tag="VFU90_05740"
     CDS             complement(940..1614)
                     /gene="rsmG"
                     /locus_tag="VFU90_05740"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5479313.1"
                     /translation="MAGELFEPLQAAGVDKPLALRLARYGALVLEANRSFNLTGAKTA
                     ADLVPHLLDSLSVAAFVGTSLCDVGSGAGLPAIPVALATGVPITLIEATRKKAAFLQR
                     VLAAFDLTGEVVAERAEIAAHRPDLREAFAAGTARAVAAAPTVAELLLPFVEIGGLAI
                     LQRGTMDERERTALTDACLMLGAEIEEERRLDEQRRIVLVRKRTATPARFPRRTGIPE
                     KRPLCT"
     gene            complement(1620..>3130)
                     /gene="mnmG"
                     /locus_tag="VFU90_05745"
     CDS             complement(1620..>3130)
                     /gene="mnmG"
                     /locus_tag="VFU90_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU5479314.1"
                     /translation="LSIATGLVETLIVEGGEVRGVACADGTRYFARRVVLATGTFLGA
                     KTFRGSVVAAEGRHGEAPAVGLARALRRLGFPTRRLKTGTPPRIDKTTIDAEAMLLQP
                     PSDTPLLFSYSSVARFGGPQLPCYVTDTNARTHALVRDNLHRSPLYGLDLIRGIGPRY
                     CPSIEDKVVKFAHNPTHQVFIEPEGWNEPTLYVGGFSTSLPADVQLAMLHTLPGLERC
                     AMLRAGYAVEYDMVPPTELQDTLETRRVAGLYHCGQLNGTSGYEEAAAQGLIAGINAA
                     RAAHGKPPVRLSRSQAYVGVMIDDLVTRGVDEPYRMLTSRAEHRVVLRHDNADLRLTP
                     VGRDAGLIDDEAWDAFQLRREAVHGGIARARRTKLGVAAIREQLFDPGTTIADALRRP
                     AIEFADVADRFEPPLEPAAGERVAIEIKIAGYVARQEAAIEKAAKTERITIPDEFDYA
                     SIAALSREAREKLSRQRPRTLGSAGRIPGVTPADVAILSLFVHRARETATAP"
BASE COUNT          486 a         1046 c         1085 g          513 t
ORIGIN      
        1 cgggccgcga actgggcggc ccggagcatg cggagcgggt cctcctcgaa cgcctccggc
       61 cgcaggacgt cgatgcgccg ggcgcggatg tcggcctctc ccccgtaggg gtcgatcagc
      121 gccccgccgg ggagcgcgcg cgcgagcatg ttcatccgaa aatcgcggcg tgcgaggtcc
      181 tcctcgagcg ggacgtcggg gccgctttgg atctcgaaat cgcggtgacc gtgaccgacc
      241 gagcgctcgc gccgcggcag gccgacgtcg accgtacggc cgtcgatgcc cagcttcaga
      301 atcgcgaagg ccgccccgac cacgtcaacc cgccccaggc ccgacaagcg acggcggagt
      361 tcctcgatcg ggaccccggt tacgaggtag tcgaggtcct tgcccgacgg ctggatgttt
      421 tcggtggcgg cgcggagctc gtcgcgcacg cggccgccga ccgcgtaaag gctgcccggc
      481 ggtaaagcgt cggccaaacg cacgtccagc gggtcggggc ggtacatcaa ggcccatcgt
      541 accgcacccg gtcgagtatt tcacgtgaaa caaggaacgg ccgcgacgcc gctcgctgcg
      601 tcatacgttt cacgtgaaac gccggtgatc ggcaaggtgc gatcggctcg ggcatcccgg
      661 aggtctgggt caagcgctgc gaccccggta ctgtcgaaga acgctttgcc ctaccggtcc
      721 tcgtccaagg gccgcgctgc tcgtgggttg cggtcgcgcc gctcgagccg gtaaacgatc
      781 cgatgccggg gcggcggctt atcgcgccgc gatcggcccg tttggccgat cgttgccggc
      841 tgcgccggaa acgggtgcct agccggaaag gggcttggat gcgaacacat gttcgagtca
      901 aattcgtaac gctcctcgta cgcttggtct tttcaagcgc tacgtgcaga gcgggcgttt
      961 ttcggggatg ccggtacggc gcggaaagcg cgccggcgta gccgtccgct tgcgaacgag
     1021 cacgatccgc cgctgttcgt cgagccgccg ttcttcttcg atttcggcgc cgagcatcaa
     1081 acacgcgtcc gtcagggcgg tgcgctcccg ctcgtccatc gtgccgcgct gaaggatcgc
     1141 gaggccgccg atctcgacga acggtaagag cagctcggct acggtcggtg cggcggcaac
     1201 ggcgcgcgcg gttccggcgg caaaagcctc gcgcagatcc ggccggtgag cggcgatttc
     1261 cgcgcgctcc gcgacgactt cgccggtgag atcgaacgcc gcgagaacgc gttgtaagaa
     1321 cgccgctttc ttgcgcgtcg cttcgatcag cgtgatcggc acgccggtgg cgagcgccac
     1381 cggaatcgcc ggcaacccgg cgcccgaacc gacgtcgcac agcgaggtgc ccacaaaggc
     1441 agccacgctt aagctgtcga gaagatgcgg caccaggtcg gccgccgttt tcgcgccggt
     1501 aaggttgaac gagcgattgg cctcgagcac cagcgcgccg tagcgcgcga gccgcagcgc
     1561 gagcggttta tcgaccccgg ccgcctgcag cggttcgaag agctcgccgg ccacctgtcc
     1621 tacggtgccg tcgcggtttc gcgcgcgcga tgtacgaaca agctcaggat cgcgacgtcg
     1681 gccggcgtta cgccgggaat tcgtccggcc gagcccagcg ttcgcggtcg ctgacgcgac
     1741 agtttctcgc gcgcttcgcg cgagagcgcc gcgatactcg cgtagtcgaa ctcgtcgggt
     1801 atcgtaatac gctcggtttt ggcggctttt tcgatggccg cttcttggcg cgcgacgtaa
     1861 cccgcaatct tgatttcgat cgcaacccgc tcgccggccg cgggttcgag cggcggctcg
     1921 aagcgatcgg cgacgtcggc gaattcgatc gcggggcgcc gcagcgcgtc cgcgatcgtc
     1981 gtgccgggat cgaaaagttg ctcgcggatc gcggcgacgc ccaacttcgt ccggcgagcg
     2041 cgcgcgatgc cgccgtggac ggcttcgcgg cgcagttgga aggcgtccca agcttcgtcg
     2101 tcgatcagtc cggcgtcgcg accgaccggc gtaagccgca ggtcggcgtt gtcgtgccgc
     2161 aacaccacgc gatgctcggc gcgcgacgtc agcatccggt agggctcgtc cacgccgcgc
     2221 gtcaccaggt cgtcgatcat gacgccgacg tacgcctgac tgcgggagag ccgcaccggc
     2281 ggtttcccgt gcgccgcgcg cgcggcgttg attccggcga tcagtccctg cgcggcggct
     2341 tcttcgtacc cggacgtgcc gttgagctgt ccgcagtgat agagtccggc gacgcgtcgc
     2401 gtttcgagcg tgtcctgcag ctcggtaggc ggcaccatat cgtattcgac ggcatagccg
     2461 gcgcgcaaca tcgcgcatcg ctcgagcccg ggcagcgtgt gcagcatcgc gagctgaacg
     2521 tcggcgggca gcgacgtgga gaagccgcca acgtagaggg tcggctcgtt ccagccctcg
     2581 ggttcgatga agacctgatg cgtcggattg tgcgcgaact tgacgacctt gtcttcgatc
     2641 gacgggcagt agcgcgggcc gatcccgcgg atcaaatcca gcccgtacag cggcgagcga
     2701 tgcaaattgt cgcgcaccag cgcgtgcgta cgcgcgttgg tgtcggtcac gtagcacggc
     2761 agctgcggac cgccgaaacg tgcgacgctc gagtaggaga agagcagcgg cgtatcgctc
     2821 ggcggttgca gcagcatcgc ctcggcatcg atcgtcgttt tgtcgatccg cgggggcgta
     2881 ccggttttca gtcgccgggt gggaaacccg agccttcgca aggcgcgtgc gagcccgacg
     2941 gcgggcgctt cgccgtggcg gccttcggcg gcgacgacgc ttccgcggaa ggtttttgcg
     3001 cccaggaacg tgccggtcgc gagcacgacg cgccgcgcga aatagcgcgt tccgtccgcg
     3061 catgctacgc ctcgaacctc gccaccctcg acgatcaacg tctcgaccag cccggtcgca
     3121 atcgaaagcc
//
LOCUS       DASUCJ010000482         1257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_20433, whole genome shotgun sequence.
ACCESSION   DASUCJ010000482 DASUCJ010000000
VERSION     DASUCJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20433"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1257)
                     /locus_tag="VFU90_05750"
     CDS             complement(<1..>1257)
                     /locus_tag="VFU90_05750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5479315.1"
                     /translation="MNLSLYGVGDERRRQWLIVDCGVSFAAEQHLPGVDLILPDIRYL
                     VAERRNIVGLVLTHAHEDHMGALIDLWPRLNVPIYATPFTAALFEARRLAEPGAPKIP
                     VNVVPLGGSVTLGPFTVDFINVAHSIPESNALAIRTPYGTVLHTGDWKIDPTPVIGAP
                     TDASRLAALGDAGVLALVGDSTNAIRDGRSPSEAEVAKTLAELILSAPARVAVTTFAS
                     HVGRLRAVANAALTAGREVVMVGRAMERVVQVARETGYLDGVQEFRGAETYGYLPPDK
                     VLALCTGSQGEPRAALARIAENAHPEVALARGDRVVFSSRVIPGNEKAVDRVINGLVN
                     QGVEVITDRSHLVHVSGHPRRAELVDMIGWVRPKILIPVHGEALHLAEHAKLARAAGV
                     PHVVVCRNGDLVRLAVEGPEIIDEVP"
BASE COUNT          222 a          420 c          420 g          195 t
ORIGIN      
        1 cgggcacctc atcgataatt tctggtccct ctacggcgag gcggacgaga tcgccgttgc
       61 ggcaaacaac cacatgcggc accccggcgg cgcgggcgag cttggcgtgc tcggcaaggt
      121 gcagggcttc gccgtggacc ggaatcaaaa tcttcggccg cacccagccg atcatgtcga
      181 caagctcggc ccgacgcgga tgtcccgata cgtggacgag gtgcgagcgg tccgtgatca
      241 cctcgacacc ctggttcacg agcccgttga tcacccgatc cacggccttc tcgttgccgg
      301 ggatgacacg ggaggagaac accacgcgat cgcctctggc cagcgcgacc tcggggtgcg
      361 cgttctcggc gatgcgcgcg agcgcggccc gtggctcacc ctggctgccg gtgcatagcg
      421 caagcacctt gtccggcggg aggtagccat aagtctccgc gccgcgaaac tcctgtacgc
      481 cgtcgagata gcctgtttcg cgtgctacct ggaccacgcg ctccatggca cgaccgacca
      541 tgaccacctc gcggccggcc gtaagcgccg cgttagccac tgcacgcagg cgtccgacgt
      601 gcgaagcaaa cgtcgtcacc gcgacacgag ccggcgcgga gaggataagc tcggctagcg
      661 tcttcgccac ctcggcttcc gagggcgaac ggccgtcgcg gatcgcattg gtggaatcgc
      721 cgacgagcgc gagcacgccg gcatcgccca gtgccgcaag tctcgacgca tccgtcggcg
      781 cgccgattac cggcgtcgga tcgatcttcc agtcgccggt gtgtagcacg gtgccgtacg
      841 gtgtgcggat cgccaacgcg ttcgattccg gaatcgaatg ggcgacgttg atgaaatcga
      901 ctgtgaacgg accgagcgtc acgctgccgc cgagcggcac cacgttcacc gggatcttgg
      961 gtgcgcccgg ctcggcgagg cgccgcgcct cgaacagagc cgccgtgaac ggcgtcgcat
     1021 agatcggcac attgagccgc ggccacaggt cgatgagcgc gcccatgtgg tcctcgtggg
     1081 cgtgggtgag cacgaggccg acgatgttgc gccgctccgc gaccagatag cgtatgtcgg
     1141 gaaggatcag atcgacaccg ggcaggtgct gctcggcggc aaaggagacg ccgcagtcga
     1201 cgatgagcca ctgccgccgc cgctcgtcgc cgacgccata gagcgaaaga ttcattc
//
LOCUS       DASUCJ010000483         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_387160, whole genome shotgun sequence.
ACCESSION   DASUCJ010000483 DASUCJ010000000
VERSION     DASUCJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387160"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VFU90_05755"
     CDS             <1..1282
                     /locus_tag="VFU90_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479316.1"
                     /translation="LAVVFRAQPAFAMRAAASGAGRGGNLSRFRATLVVAEVAMALVL
                     MSAATSLLGTFVHLQHVDLGFRPDSTFVSRVSLSGTKYPERSDLVLFYERMQKALAAI
                     PGVEAAGMVSVAPFSGVIATETFDVVGRESATARDRPFANYRAISPQYLSTIGARLVA
                     GRMLREDDGETTQPVALVNQTVANRYFAGASPVGKDIWIDDDGVARRRVHIVGVVANL
                     RQVTLDGPATNDVFVPLRQLPKAAIPFVVATSFWSVRTRTQPASFAPTFRRLLNAVDP
                     DAASSGSTSLREYVDDSLAPRRYGVTILVIFVGLSIFLAALGVHGVMRFDVEQRRREI
                     AVRMAFGATTLRIVGWTLRRMLLVAGGGLLLGIVGSLVAGQLIAGLLFGTTPYNPVFL
                     AASSALILVVALVATAVPAVVAARTAPSVVLSSS"
BASE COUNT          236 a          518 c          518 g          336 t
ORIGIN      
        1 actcgctgtc gtctttcgtg cgcaaccggc gttcgcgatg cgagccgccg caagcggtgc
       61 aggacggggc ggcaacctct cgcgatttcg cgccacgctc gtcgtggcgg aggtcgccat
      121 ggcgctcgtg ttgatgagcg ccgcgacgtc tctactcggc acgttcgttc acctccaaca
      181 tgtcgatctg gggttcagac cggattccac attcgtgtcc agggtgtcct tgtccggcac
      241 caagtaccca gagcgttccg atctcgttct cttctacgag cggatgcaga aggcactcgc
      301 ggcgattccg ggggtggaag cggccggcat ggtctccgtg gcgccgttca gcggtgtcat
      361 cgcgaccgag acgttcgacg ttgttggacg cgagtcggcg acggcccgag accgcccgtt
      421 cgcgaactat cgagcgatca gcccacagta cctctccacc atcggcgcga ggctcgtcgc
      481 tggtcgcatg ctccgtgagg acgatggcga gacgacccag cccgtcgcgc tcgtcaatca
      541 gaccgtcgcg aatcggtatt tcgccggcgc ctcaccagtg ggcaaggaca tctggatcga
      601 cgacgacggc gtcgcgcgcc gacgagttca cattgttggc gtcgtggcca acctgcgtca
      661 ggtgaccctt gacggaccag ccaccaacga tgttttcgtg ccgctgcgtc aactcccgaa
      721 agccgccatt ccgtttgtgg tcgcgacttc gttttggagc gtccgcacgc gaacgcagcc
      781 ggcgagcttc gcccccacct tccggcgcct gctgaatgcg gtcgaccccg acgctgcgag
      841 ctccggttcg accagtctgc gagaatacgt tgacgatagc ctggcgccgc ggcgctacgg
      901 ggtcacgatc ctcgtgatct tcgtcggact ctcgatcttc ttggccgcac tcggcgttca
      961 cggagtgatg cgtttcgacg tcgagcagcg acggcgggag atcgcggtgc gcatggcgtt
     1021 tggcgcaacc acactccgca ttgtcggatg gactctacgg cggatgttgc tggtcgccgg
     1081 cggtggcctg ctgctgggaa ttgtcggatc gcttgtcgcc ggtcagctga ttgctgggct
     1141 gttgttcgga acgacgccgt acaatccggt gttcctcgcg gcaagctccg ccctgattct
     1201 cgtcgtcgca ctggtcgcga ccgccgttcc agccgttgtg gccgcgagga ccgctccctc
     1261 agtcgttctt tcctcgtcct gagttcgatg cttggatgag cggctgacgc cgtgttccgc
     1321 gcgatctgac cgagccaatc tgtaaatggc cgccgatccc gtatcgtagc gcgagctaga
     1381 cgacgcggct cggcgttgtc atctgcgact ccgttgagcg gttgggcgcg ccggcggaac
     1441 ttatgcgccg tgcgcgtatc tgcctcgctg ctgtcacaac ggcggcgggg ttggcatgcc
     1501 gtttcgagta gatgagcgat cccgttcttg aaggcgatcg agaccgcgca ggccgaggcc
     1561 tctcgttcta cggtcccgat ggcgcgtcgc cctgcataat gcggagca
//
LOCUS       DASUCJ010000484         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_219193, whole genome shotgun sequence.
ACCESSION   DASUCJ010000484 DASUCJ010000000
VERSION     DASUCJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219193"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFU90_05760"
     CDS             complement(<1..926)
                     /locus_tag="VFU90_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479317.1"
                     /translation="MRLSTHALTVGAIASFAQLNSGTWAAQGSLGGAVYSPSLGPLRG
                     EFDVDAAGSDRQDGTQTGQLEGFARVDLTRAAWGAWLGGGAGRAWDGFSGHSLVLADA
                     GLWLGRHALTANLSATPTAIDDSIQYTDFEAAARWARARLELRASLGARAGHALAAAS
                     RGRTWGSAGALYWVGPRIGISADAGLYPPDIAQGFPNGRYVSLGVRLSVHPMWRSRIP
                     TSSGTDLSEQSGAASADAGMIEGFDVESAGTQRTIRVHAPHARLVEFASDVTNWSPVA
                     LSRDRAGSWTGTFTVAPGIHQVSLRVDGQKWVV"
BASE COUNT          160 a          357 c          337 g          156 t
ORIGIN      
        1 acgacccact tttgcccgtc cacacgcagg ctcacctgat ggattcctgg cgccaccgtg
       61 aacgtacccg tccacgagcc tgcgcgatcc cgcgacaatg cgaccggact ccagttcgtc
      121 acgtcgcttg cgaattcgac aagccgcgca tgtggtgcgt gcacgcggat cgtgcgttgc
      181 gtccccgcgc tctctacatc gaaaccttcg atcataccgg cgtccgctga agcggcgccc
      241 gactgctcgc tcaagtcggt ccccgaactg gtcggaatac gcgatcgcca catggggtgc
      301 acgctcaatc gcacgccgag cgatacgtag cggccgttgg gaaatccttg tgcgatgtcc
      361 ggcggataca gaccggcgtc ggccgagatt ccgatgcgcg gccccaccca gtacagggcg
      421 ccggccgaac cccacgtgcg gccccgcgac gccgcggcga gtgcgtgtcc cgctcgcgcg
      481 ccgagcgatg cgcgcagctc gagtcgtgcg cgcgcccacc gagcggcggc ctcgaagtcg
      541 gtgtactgga tgctgtcgtc gatcgcggtt ggcgtggcgc tgagattggc cgtgagtgcg
      601 tgtcggccga gccagaggcc ggcgtcggcc agcacgagcg agtgccccga gaacccgtcc
      661 cacgcgcggc cggcgccgcc gcctaaccag gcaccccacg cggcgcgcgt caggtcgacg
      721 cgggcgaatc cctcgagctg accggtttgc gttccatcct ggcgatcgga tccagcggcg
      781 tcgacgtcga actccccgcg caggggtccc agcgacggcg agtagacggc cccgcctaac
      841 gaaccctgtg cggcccaggt tccggagttg agttgcgcga aactggcgat ggcgccgacc
      901 gtgagtgcgt gcgtcgagag ccgcacgctc ggcgatagaa ctgctgcgcc ggcgtttacg
      961 gaatcgctat agcgaatacc cgcggcgccg gcgtccaggg tcgcatcgat
//
LOCUS       DASUCJ010000485         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_861048, whole genome shotgun sequence.
ACCESSION   DASUCJ010000485 DASUCJ010000000
VERSION     DASUCJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861048"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VFU90_05765"
     CDS             complement(<1..1499)
                     /locus_tag="VFU90_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5479318.1"
                     /translation="MQTIQPATPPRVDIAELLRNLPRIGGTIPMYLDGRWKLAEDGGT
                     RDLANPANGETIVTVAEGAAADAELAIRSARKAFDEGPWRETSAADRAALLLRVADAI
                     DASRDEFVRIDTLNNGKPLRECEYDAIDAANCFRYYAGLATKPHGQTFDVPAPSQTFT
                     VREPIGVCGQIVPWNYPLLMAVWKLAPALAAGNVCVLKPSELTPLSALRLAAIFEQLE
                     FPPGVVNVVTGAGATVGHTIAVSPLVDKIAFTGGTKTGRSIMQDAAATLKRVSLELGG
                     KSPNVVFADAEFETAVDYALYGIYANAGQVCSAGSRLLLEESLHDRFVERLVERARKI
                     RVGDGFDPSTEMGPIISPVHRQRIEDYIKVGREEGASLVCGGQRLGGALAAGNFIEPT
                     VFDRVDPQMRIVQEEIFGPVLVVQTFKDEADAVRLANDTVYGLAGAVFTEDVAKAHRV
                     IRKLRAGITWINTYHPTYNEAPWGGYKQSGFGRELGTYGYDAYTEVKQI"
     gene            complement(1507..>2433)
                     /locus_tag="VFU90_05770"
     CDS             complement(1507..>2433)
                     /locus_tag="VFU90_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5479319.1"
                     /translation="TEAVEGALKLARAYDPTKQTIVAATKGFHGKSYGSLSASAKAEF
                     RRPFGPMLPNIEHVPFNDLLALADMMTSCKAVGEDVGAVLLEPIQGEGGVNIPSDDYL
                     PGVRALCDEFGALLILDEVQTGMGRTGTMFCCERYGVAPDLMCLAKAFGGGVMPAGAV
                     VGRREIFARLFDNPFLHTSTFGGNPLACAAALATINVLIEERLPERATKMGERMLAGI
                     RRGVAGHEDIVADVRGMGLLIALEFHDHAAGFEFGKRMLDRGVLVSGTLVNARTIRIE
                     PPLTITEEQADYVCQAIADSLNAMSAFAKTGV"
BASE COUNT          399 a          776 c          807 g          451 t
ORIGIN      
        1 ttgatctgct tgacttcggt atatgcgtcg tagccgtagg tgccgagctc gcggccgaaa
       61 ccggattgtt tgtagccgcc ccagggcgct tcgttgtagg tcggatgata ggtgttgatc
      121 caggtaattc cggcgcgcag cttgcggatc acgcgatgcg ccttagcgac gtcttcggtg
      181 aacacggcac cggccaatcc gtagactgtg tcgttggcca gccgcaccgc gtcggcttcg
      241 tctttgaacg tttgcaccac caataccggg ccgaagattt cttcttgaac gatgcgcatt
      301 tgcggatcga cccggtcgaa cacggtgggc tcgatgaagt tcccggcggc cagcgcaccg
      361 cccaaacgtt gcccgccgca caccagcgac gcgccctcct cgcggcccac cttgatgtag
      421 tcttcgatgc gctggcgatg caccggcgag atgatcggcc ccatctcggt gctcgggtcg
      481 aagccgtcgc cgacgcgaat cttgcgcgcg cgctcgacca ggcgttcgac gaaacgatcg
      541 tgcagcgatt cctcgagcag caggcgcgag cccgccgagc agacctgccc ggcgttggcg
      601 tagatgccgt acagcgcgta atcgaccgcc gtttcgaatt cggcgtcggc gaagacgacg
      661 ttcggcgact tcccgcccag ctcgagcgag acgcgcttga gcgtcgcggc cgcgtcttgc
      721 atgatgctgc ggccggtctt cgttccgccg gtaaaagcga ttttgtcgac cagcggactg
      781 accgcgatcg tgtggccgac ggtggccccg gctccggtga cgacgttgac gacgccgggc
      841 ggaaactcga gctgttcgaa gatcgccgcg agccgcagcg ccgagagcgg cgtcagctcg
      901 gagggtttga gcacgcacac gttcccggcc gccaacgcgg gagcgagctt ccacaccgcc
      961 atcagcagcg gatagttcca cggaacgatt tggccgcata cgccgatcgg ctcgcgcacc
     1021 gtgaacgttt gcgacggtgc cggcacgtcg aaggtttgac cgtgcggttt cgtcgctaac
     1081 ccggcgtaat agcgaaaaca gttggccgca tcgatcgcgt cgtactcgca ctcgcgcagc
     1141 ggtttgccgt tgttgagcgt gtcgatgcga acgaactcgt cccggctcgc atcgatcgcg
     1201 tcggccaccc gcaggagcag cgcggctcga tcggcggcgc tggtttcgcg ccagggacct
     1261 tcgtcgaagg ccttgcgcgc ggaccggatc gccaactcgg cgtcggccgc ggctccctcg
     1321 gcaacggtaa cgatcgtctc gccgttggcg gggttggcga ggtcgcgcgt gccgccgtcc
     1381 tccgcgagtt tccagcgccc gtcgaggtac atcggaatgg tgccgccgat gcggggaagg
     1441 ttgcggagca gctcggctat gtcgacgcgc ggaggcgttg ccggttgaat cgtttgcatg
     1501 atggatttag actccagtct tggcgaatgc ggacatcgcg ttcaaactgt cggcgatggc
     1561 ttgacacacg tagtcggctt gttcctccgt aatcgtcaac ggcggctcga tccggatcgt
     1621 gcgcgcgttg acgagggtcc ccgacacgag cacgccgcga tcgagcattc gcttgccgaa
     1681 ctcgaagccg gcggcgtgat cgtgaaactc cagcgcaatc agcaaaccca ttccgcgcac
     1741 gtcggcgacg atatcctcgt gtccggcgac gccgcgccgg attccggcga gcatgcgctc
     1801 gcccatcttg gtcgcgcgct cgggaaggcg ttcttcgatt aggacgttga tggttgcgag
     1861 cgccgcggcg caggccaagg ggttgccgcc gaacgtcgac gtgtgcagga acgggttgtc
     1921 gaacaggcgc gcaaaaatct cgcggcgtcc gacgaccgcg cccgcgggca tgacgccgcc
     1981 gccgaaggcc ttggccagac acatgaggtc gggggcgacg ccgtagcgtt cgcagcagaa
     2041 catcgtgccg gtgcgtccca ttccggtttg gacttcgtcg aggatcagca gcgcgccgaa
     2101 ctcgtcgcac agcgcgcgta ctcccggcag gtaatcgtcg gacggaatgt tgacgccgcc
     2161 ttcgccttgg atcggctcga gcagcaccgc tccgacgtcc tcgccgaccg ctttgcacga
     2221 ggtcatcatg tcggccagcg cgagcaagtc gttgaacgga acgtgctcga tgttcggcag
     2281 catcgggccg aacggacgcc ggaactcggc tttggcgctg gccgaaagcg agccgtagct
     2341 cttgccgtga aagcctttgg tcgcggcgac gatcgtctgc ttggtgggat cgtacgcacg
     2401 agcgagtttc aacgcgcctt cgacggcttc cgt
//
LOCUS       DASUCJ010000486         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1411241, whole genome shotgun sequence.
ACCESSION   DASUCJ010000486 DASUCJ010000000
VERSION     DASUCJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411241"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..155)
                     /locus_tag="VFU90_05775"
     tRNA            complement(84..155)
                     /locus_tag="VFU90_05775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Thr,seq:cgt)
     gene            313..960
                     /locus_tag="VFU90_05780"
     CDS             313..960
                     /locus_tag="VFU90_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479320.1"
                     /translation="MKITAMLAALVALAVPASALADQDATYHGPLSPGKNAFVLSIRS
                     DLTKRFPTAADAEKAGYVRYTNADDSGAVSYANQQWQSADIRHPSQLWYDKNGRLLGA
                     DYSVLLSTSPARPQLWGVNPGRWVEFDGHVHWVAKNPATGALTYDHWIMDPKFRAAGG
                     DPEHPTAEALVAAKKVAAASDVVTIFHFPSLWDLIVWVLPNPNGAFADKNPNVKP"
     gene            complement(970..1236)
                     /locus_tag="VFU90_05785"
     CDS             complement(970..1236)
                     /locus_tag="VFU90_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479321.1"
                     /translation="MHRHRTPLRGVKAIPHPRPDDPNYTSLNYEYADREGHIHEVVEI
                     GGRQLAKIGFPDRKIVYYLLDDVELDESATRGTFHDDDVRRGKD"
     gene            1278..1820
                     /locus_tag="VFU90_05790"
     CDS             1278..1820
                     /locus_tag="VFU90_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5479322.1"
                     /translation="MLITYRGKRPAVDPSAFVAPNAVLIGDVEIGPESSVWFGAVLRG
                     DNGPIRVGARTSIQDNAVVHVSEHGGTYIGDDVTVGHCAVMEDCDIKRYALIGSNATL
                     LNGCTIGEGALVAAGSVVGERAEIPPRVVAAGAPARVKKTLEGEAANWVEISAGEYVK
                     LSRSYLAQSIGAVEDQETTI"
     gene            1820..2284
                     /locus_tag="VFU90_05795"
     CDS             1820..2284
                     /locus_tag="VFU90_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479323.1"
                     /translation="MSVRDRIARFVESEGHRVEVTFVADAPELAELVFRTRGQTYSVA
                     VSELEPQRFSLSTAYEVPDSMRDPAQTRDALGDVAGSFPETRFSLAHDDTIFIATVEY
                     EAPDVEAFLENFWAIVGRLRDAGTDAIEHMVDRSESKAAADKFIRQFMKGER"
     gene            2281..2691
                     /locus_tag="VFU90_05800"
     CDS             2281..2691
                     /locus_tag="VFU90_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEU5479324.1"
                     /translation="MNDADHLLPVESAIDDEGLLFTRDNDDEALRITFKSEGDRYFVV
                     GYRDDPNFVMIGSGWALPPDVELPEARAAANALNVRKKFVKTAIWEEERDALFTVELC
                     VENSIEIHPHFGRLLDALRDTALEFFNELRDDGD"
     gene            complement(2694..>3143)
                     /locus_tag="VFU90_05805"
     CDS             complement(2694..>3143)
                     /locus_tag="VFU90_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5479325.1"
                     /translation="CRLLKKSTGSNLGAIFIDYLQLMRPSLLTRNTNRNEELSEICRT
                     LKITAKELNVPIVALAQLNRAVETRADKRPMLADLRDSGSIEQEADVVAFLFREGYYN
                     PEGSDPDVTEFIIAKHRNGPTGHVRLRFQREFTLFVPYGDDSHYPSP"
BASE COUNT          539 a         1024 c         1032 g          548 t
ORIGIN      
        1 agcagcgcta ccctggtcgt cttggcaaac gctgggatca tgcctcccat ttattgggtc
       61 acagcttaga tattttttgg gtaagccgac ggccgggatt gaaccggcga cctattgttt
      121 acgaaacaat tgctctaccg ctgagctacg tcggcgcgta cgtcggcaac ccggagaatt
      181 gccgacggcc ctggttccac gacgcgagtt gggcccctgc ccgcgccggt tcgtgcctcg
      241 ggactgcttc gccggcactt tgatgcgctc gtgatggttt ccggcagcgc ccggggttac
      301 gatacccgca ctatgaagat caccgccatg ctcgcggcgc tggtagcgct cgcggtcccc
      361 gcctccgcgc tggccgatca agacgccacc tatcacggcc cgctcagccc tggcaagaac
      421 gccttcgtcc tctcgattcg ttccgatctc accaagcggt tcccgaccgc tgccgacgcc
      481 gagaaggccg gctacgtccg ctataccaac gcggacgaca gcggcgccgt cagctacgcg
      541 aaccagcagt ggcagtcggc cgacatccgc caccccagcc aactctggta cgacaagaac
      601 ggccggctgc tcggcgccga ctattcggtc ctgctctcga ccagcccggc ccgtccgcag
      661 ttatggggcg tcaacccggg gcgctgggtt gagttcgacg gccacgtgca ctgggtcgcg
      721 aagaacccgg cgaccggcgc gctgacctac gaccactgga tcatggatcc gaagttccgc
      781 gccgcgggcg gcgatccgga gcatccaacc gccgaggcgc tggtggccgc caagaaggtc
      841 gccgccgcat ccgacgtcgt cacgatcttc cactttccgt cgctgtggga cctgatcgtc
      901 tgggtgcttc cgaacccgaa tggcgcgttt gccgataaga acccgaacgt gaagccgtaa
      961 cctccggcgt cagtctttcc cgcgacggac gtcgtcgtcg tgaaaggtgc cgcgcgtcgc
     1021 gctctcgtcg agttccacgt cgtcgagcaa gtagtagacg atcttccggt cgggaaagcc
     1081 gatcttcgcc aactgccgcc cgccgatctc gacgacttcg tggatgtgcc cctcgcgatc
     1141 ggcgtattcg tagttgagcg acgtgtagtt cggatcgtcg gggcgcggat ggggaatggc
     1201 cttcaccccg cgcagcgggg tccggtgcct atgcatgcgg gcgaggttcg acggccgggg
     1261 ctgcgaagca tgggcgtatg ctgatcacct accgaggtaa acgcccggcg gtcgacccct
     1321 cggcgttcgt cgcgcccaac gccgtgctga tcggcgacgt cgaaatcggc cccgaatcga
     1381 gcgtgtggtt cggtgccgtg ctgcgcggcg acaacgggcc gatccgcgtc ggcgcgcgca
     1441 cttcgattca agacaacgcg gtcgtgcacg tgagcgagca cggcggcacc tacatcggcg
     1501 acgacgtgac cgtcgggcac tgcgcggtta tggaagactg cgacatcaag cggtacgcgc
     1561 tgatcggcag caacgcgacg ctgctgaacg gctgcacgat cggcgaaggg gcgctcgtcg
     1621 ccgccggcag cgtggtcggc gaacgcgccg agatcccgcc gcgggtggtc gccgccggcg
     1681 cgccggcccg cgtgaaaaaa acgctcgagg gcgaagccgc gaattgggtc gagatttcgg
     1741 ccggcgagta cgtcaagctc tcgcgatcct acctcgccca gagcatcggt gccgtggagg
     1801 accaggagac cacgatttag tgagcgtccg cgaccgaatc gcgcgattcg tcgaaagcga
     1861 aggtcatcgc gtcgaggtga ccttcgtcgc cgacgcgccc gagctcgccg agctggtgtt
     1921 tcgcacgcgc gggcagacgt actccgtggc cgtgtccgag ctggagccgc agcgcttcag
     1981 tttgtccacc gcgtacgagg tgcccgactc gatgcgcgac ccggcgcaaa cgcgcgacgc
     2041 gctcggcgac gtcgccggca gctttcccga gacccgcttc tcgctcgcgc acgacgacac
     2101 gatcttcatc gcgaccgtgg aatacgaggc ccccgacgtc gaggcgttcc tcgaaaactt
     2161 ttgggcgatc gtcggccggc tgcgcgacgc cggtaccgac gcgatcgagc acatggtcga
     2221 ccgcagcgag agcaaggccg cggccgacaa gttcattcgc cagttcatga aaggcgaacg
     2281 gtgaacgacg ccgatcacct cctgccggtc gagtcggcga tcgacgacga agggctgctg
     2341 tttacgcgcg ataacgacga cgaggcgctg cgcatcacgt tcaagagcga gggcgatcgc
     2401 tacttcgtgg tcggctatcg cgacgatccg aacttcgtga tgatcggctc cggttgggcg
     2461 ctcccgccgg acgtcgagct gccggaagcc cgcgcggccg ccaacgcgct caacgtgcgc
     2521 aagaagttcg tcaagaccgc gatctgggaa gaggagcgcg acgcgctgtt caccgtggag
     2581 ctgtgcgtag aaaactcgat cgaaatccat ccgcacttcg ggcggctgct cgacgcgctg
     2641 cgcgacaccg cgctcgagtt cttcaacgag ctccgcgacg acggcgatta acgctagggg
     2701 gacgggtagt gcgagtcgtc tccgtacggc acgaacaggg tgaattcgcg ctggaagcgc
     2761 agccgcacgt ggccggtcgg gccgttgcgg tgcttggcga tgatgaactc ggtgacgtcg
     2821 ggatcgctgc cttcgggatt gtagtatccc tcgcggaata gaaacgcgac cacgtcggct
     2881 tcctgttcga tcgaacccga atcgcgcagg tccgccagca tcgggcgctt gtcggcccgc
     2941 gtttcgacgg cgcgattgag ctgcgcgagc gccacgatcg gcacgttgag ctccttggcg
     3001 gtaatcttca gcgtgcggca aatctccgac agctcttcgt tgcggttggt gttgcgcgtc
     3061 agcaagctgg ggcgcatcag ctgcaagtag tcgatgaaga tcgcgccgag gttggaaccg
     3121 gtgcttttct tcagcagccg gca
//
LOCUS       DASUCJ010000487         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1304217, whole genome shotgun sequence.
ACCESSION   DASUCJ010000487 DASUCJ010000000
VERSION     DASUCJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304217"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VFU90_05810"
     CDS             complement(<1..1544)
                     /locus_tag="VFU90_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5479326.1"
                     /translation="MHLTRLDWLIIVLSLGVTFVPAIVLARRAGRNITEFFAAGRQAP
                     WWLIGISLVATTFSTDTPNLVTNLVRDGGVAENWVWWSFLLTGMATVFFYARLWRRSG
                     VLTDLEFYELRYSGRAASFVRGFRAVYLGLFFNCVIIATVNLAAVKIAGVLFGWPRFE
                     TLLLLAAVPIVFAATAGLWGVMVTDMIQFCITITSAFAAAYFAVHAPGVGGLHGLLAH
                     FHGTNASLLSILPNFADWPTALAIFVIPLTVQWWSVWYPGAEPGGGSYVAQRMLAART
                     ESDALYGTLAFQVMHYALRPWPWIVVALASTIVYPSLHDIAARFPSVQPSLIGNDIAY
                     PAMLVFMPAGFAGFMVAGLFAAYRSTIETHLNWGTSYLVHDFYQRFVRPGSTQRELVL
                     AGRIVTVLLMAAGVVFSLYLDTAKDAFNLLLSIGAGTGLIYLLRWFWWRINAWSEVSA
                     MFASFAVALAFFVAAKLGHRVADTTVLLTTVGVTTVAWLAVTFATPPVERAALAAFYA
                     KVRPAGP"
     gene            1539..>1744
                     /locus_tag="VFU90_05815"
     CDS             1539..>1744
                     /locus_tag="VFU90_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479327.1"
                     /translation="MHGRARYGQAARAPVDGDGAYNAGNGASLNGGQYVWQFTAKQRA
                     ARLHPIKSGSDGSCGGSYLCQAGT"
BASE COUNT          386 a          576 c          559 g          223 t
ORIGIN      
        1 gggccggccg gccggacctt ggcgtaaaag gcggccaaag cggcccgctc gaccggcggc
       61 gtggcaaacg ttaccgcgag ccacgctacg gtggtcaccc ccaccgtggt cagcagcacc
      121 gtcgtatcgg cgacccgatg accgagtttg gcggcgacga aaaacgccag tgccaccgca
      181 aacgatgcga acatcgcgct gacttcgctc cacgcgttga tccgccacca aaaccagcgc
      241 agcaaataaa tcagcccggt gccggcgccg atcgacagca gcagattgaa cgcgtccttc
      301 gcggtgtcca ggtagagcga aaagacgacg ccggccgcca tcaacagcac ggtcacgatc
      361 cgccccgcta aaaccagctc gcgctgcgtc gaacccggcc gcacgaaccg ttgatagaaa
      421 tcgtgcacga ggtaggacgt gccccagttc aggtgcgttt cgatggtcga ccggtaggcc
      481 gcaaacaaac cggcgaccat gaagcccgca aatccggccg gcataaagac gagcatcgcg
      541 ggataggcga tgtcgttgcc gatcagcgac ggctgcaccg acgggaatcg cgcggcgatg
      601 tcgtgcagcg aaggatagac gatggtcgac gccagtgcga cgacgatcca cggccagggc
      661 cgcagtgcgt agtgcatcac ttgaaacgcc agcgttccgt agagtgcgtc ggattcggta
      721 cgcgccgcga gcatgcgctg cgcgacgtaa ctcccgccgc cgggctcggc gcccggatac
      781 cacaccgacc accactgcac ggtcagcgga atcacgaaaa tcgcgagcgc ggtcggccaa
      841 tcggcaaaat tcggtaaaat cgacagcagg ctcgcgttcg taccgtggaa atgtgccagc
      901 agcccgtgca gcccgccgac cccgggagcg tggacggcaa aatacgccgc ggcgaacgcc
      961 gacgtgatcg tgatgcagaa ctggatcatg tcggtgacca ttacgcccca gagcccggcg
     1021 gtggctgcga acacgatcgg caccgccgcg agcagcagca gcgtttcgaa gcgcggccag
     1081 ccgaacagca cccccgcgat cttgacggcc gcgaggttca cggtcgcgat gatgacgcag
     1141 ttgaagaaca gccccaggta gacggcgcgg aacccgcgca cgaacgatgc ggcgcgcccc
     1201 gaatagcgca gttcgtagaa ctcgaggtcg gtcaagacgc cgctgcggcg ccacagccgc
     1261 gcgtaaaaga acaccgtcgc catcccggta agcaggaacg accaccagac ccagttttcg
     1321 gcgaccccgc cgtcgcgcac caagttggtg acgaggttcg gagtatcggt ggaaaacgtg
     1381 gtcgcgacca gcgaaattcc gatcagccac cacggcgctt ggcggccggc ggcaaagaac
     1441 tcggtaatgt tgcggccggc gcggcgcgcg agcacgatgg ccggaacgaa cgtcacgccg
     1501 agcgagagca cgatgatcag ccaatcgagc ctcgtcagat gcatggacgg gcgcgttacg
     1561 gccaagccgc gcgcgctcct gttgatggcg acggcgctta caacgccggc aacggcgcca
     1621 gcctgaacgg cggtcaatac gtctggcagt tcaccgccaa acaacgcgcc gcgcgtctgc
     1681 atccgataaa gagcggcagc gacggcagct gcggcggaag ctacctctgc caggccggca
     1741 ccaa
//
LOCUS       DASUCJ010000488         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1166790, whole genome shotgun sequence.
ACCESSION   DASUCJ010000488 DASUCJ010000000
VERSION     DASUCJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166790"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFU90_05820"
     CDS             complement(<1..334)
                     /locus_tag="VFU90_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479328.1"
                     /translation="MRPYVLLVASVAALGGLLFGYDTGVISGAILFISKDFSLSTRVQ
                     EFTISVVLLGCILGAAVTGTVADAIGRRLTLLAAGAVFLIGAIVSAFAPHEATLLAGR
                     FVVGIGVGL"
     gene            375..1214
                     /locus_tag="VFU90_05825"
     CDS             375..1214
                     /locus_tag="VFU90_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5479329.1"
                     /translation="MEIASALEYARRHALEAFVAMRGDDVVAETYDGGFTSKKPHPLY
                     SGTKSFWGVTACCAQRDGLLELDEPAAATLESWRGDPWKRRVTLRMLLSLTSGFGFGG
                     LGSAVPPYARAVATALKNEPGTTFTYGGIPLQVFGAVLAHKLAARKQTPHAYLRERIL
                     DPAGVAVASWRTLADGTQPLPTGASLTAADWLRYGRFVLRERAAFDACFHGSPANARY
                     GLGWWLGSPGAPSDLFYASGSGGQALYVAPSLDLAIVHFGKSASYKHDALLKRLFATP
                     ASD"
     gene            complement(1223..>2791)
                     /locus_tag="VFU90_05830"
     CDS             complement(1223..>2791)
                     /locus_tag="VFU90_05830"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479330.1"
                     /translation="DRGERWTPISPDLTLDLKEHQKPAGGPLAYDVSGAEYSDTILSI
                     EGSPIGSGEIWVGTDDGLVQLTRDGGAHWQNVTPPGVPPFGRVETAAPSPLVAGTAYV
                     NIDRHRSGDYAPYLFVTHDYGKTWTKIVAGLPADQYVRTVRGDSVNSNLVYAGTERGL
                     YLSYDGGNHWQNFRLNLPPVSVRDVRVQPQFDDLLLATHGRSLWILDDAASLQNLPAA
                     QAAGVYLFPPRVAYEYHLHANDEGTYTRFAGANPPLGAIVDFYQQKPGAKAPAIDILD
                     ASGNVIRRVSGTHKERGKDVPDVTNKAGINRYVWDFREDGPVLWTGAAREAYRGPKVG
                     ALVVPGTYSVRVVLDGRTLAQKFTVKPDPRDAWTQADYQAGYAFAKKYLGDYSQIDRV
                     LNNLDALKKSLESAERAAAKAGDSALAAQLRDAETARAGIFALFTADYHNDEDSIQRP
                     GALREDVPSGGFLLRGNQPPTPALLEYAARFDAAYAAAFAKYDGMVSNLAPLQSQLKS
                     AGLPALQGAAPVSP"
BASE COUNT          443 a          950 c          939 g          459 t
ORIGIN      
        1 tgaggcccac gccgatgccc acgacgaagc gcccggctag gagcgtcgct tcgtgcggcg
       61 caaacgccga aacgatcgcg ccgatcagga agacggcgcc cgccgccagc agcgtcagcc
      121 gccggccgat ggcgtcggcg acggtacccg tcaccgccgc gccgagaatg cagccgagca
      181 gcacgacgct gatcgtgaac tcttggacgc gcgtcgacag cgagaagtct ttgctaataa
      241 agagaatcgc gcccgaaatg acgcccgtgt cgtaaccgaa gagcagcccg ccgagagccg
      301 cgacggacgc caccaagagg acgtacggcc tcatggaggc gcttttgcgg cagctgcgcg
      361 aacgctcctt ccctatggag atcgcctccg cgctcgagta cgcacgccgg cacgcgctcg
      421 aggcgttcgt cgcgatgcgc ggcgacgacg tcgtcgcgga aacgtacgac ggcgggttca
      481 cttccaagaa gccgcacccg ctctacagcg gaaccaagag cttttggggc gtcaccgcct
      541 gctgcgcaca acgcgacggc ctgctcgagc tcgacgagcc ggcggccgca acgctcgagt
      601 cgtggcgcgg cgatccttgg aagcggcgcg tgacgctgcg gatgctgctc tcgctaacgt
      661 ccggcttcgg cttcggcggc ctcggaagcg cggtcccgcc ctacgcccgc gccgtggcga
      721 cggcgttgaa gaacgagccg ggaacgacgt ttacctacgg cggcatcccg ttgcaagtct
      781 tcggcgccgt gctggcgcac aagctcgccg ctcgcaagca aactccccac gcctacttgc
      841 gcgaacgcat tctcgatccg gccggcgtcg cggtcgcgtc gtggcgcacg ctcgccgatg
      901 gaacgcagcc gctcccgacc ggcgcttcgc tgaccgccgc cgactggctg cgctacggac
      961 gcttcgtgct gcgcgagcgc gcggcgttcg acgcatgctt tcacggctcg cccgccaacg
     1021 cgcgctacgg tctgggctgg tggctcggtt cgcccggcgc gccgagcgat ctcttctatg
     1081 caagcggctc gggcggccag gcgctctacg tggctccgtc gctcgacctg gcgatcgtcc
     1141 acttcggcaa gagcgcgtcg tacaagcacg acgcgctcct gaagcgtctg ttcgctacgc
     1201 cggcgtcaga ctgacgccgg cctcacgggg acaccggagc ggcgccctgc agcgcgggca
     1261 agcctgcgct cttgagctgc gactgcaacg gcgcgaggtt cgacaccatc ccgtcgtact
     1321 ttgcaaacgc tgccgcgtac gccgcgtcga aacgcgccgc gtattcgagg agcgccggcg
     1381 tcggcggctg atttccacgc aataagaacc cgccgctagg cacgtcctcg cgcagcgctc
     1441 ccggccgctg gatcgagtct tcgtcgttgt ggtagtcggc ggtgaagagc gcaaagatgc
     1501 cggcgcgggc ggtttcggcg tcgcgcagct gcgcggccaa tgccgaatcg ccggctttag
     1561 cggcggcccg ttccgcggac tcgagcgatt tcttcagcgc gtcgagattg ttcagcaccc
     1621 ggtcgatctg ggaatagtcg cccagatact ttttggcgaa ggcatagccg gcttggtagt
     1681 cggcctgcgt ccacgcatcc cgcggatccg gcttcaccgt gaacttctgc gcgagcgtgc
     1741 gcccgtccag aacgacgcgc acggagtagg tgcccggcac caccagcgcg ccgaccttcg
     1801 ggccgcggta ggcttcgcgc gcggcgccgg tccatagcac gggtccgtcc tcgcgaaagt
     1861 cccaaacgta acggttgatg cccgccttat tcgtcacgtc ggggacgtct ttgccgcgct
     1921 ctttgtgcgt accggagacg cggcgaatca cgtttccgga agcgtcgagg atatctatgg
     1981 ccggcgcttt cgcgccgggt ttttgctggt agaaatcgac gatcgcaccg agcggcgggt
     2041 tggcgcccgc gaagcgcgtg tacgttccct cgtcgttggc gtggaggtgg tattcgtacg
     2101 cgacccgcgg cgggaagaga tacacgcccg ccgcctgcgc cgccgggagg ttctgcagcg
     2161 acgcggcatc gtcgagaatc cacagcgatc gtccgtgcgt cgcgagcagc aggtcgtcga
     2221 actgcggctg cacgcggacg tcgcgcaccg agaccggcgg caggttcaag cgaaagtttt
     2281 gccagtggtt accgccgtcg taggagagat acaacccgcg ctcggttccg gcgtagacga
     2341 gattcgaatt gaccgagtcg ccgcgcacgg ttcgcacgta ctggtcggcc gggagacccg
     2401 caacgatctt cgtccaggtc ttcccgtaat cgtgcgtcac gaacagatac ggcgcgtagt
     2461 cgccggagcg atgacgatcg atgttgacgt aggcggtgcc ggcgaccagc ggcgacggcg
     2521 cggcggtttc gacccgtccg aacgggggca cgccgggcgg cgtcacgttt tgccagtgcg
     2581 cccccccgtc gcgggtgagc tgaacgaggc cgtcgtcggt cccgacccaa atctcgccgc
     2641 tgccgatcgg cgacccttcg atcgagagaa tcgtgtcgga gtactcggct cccgaaacgt
     2701 cgtacgccag cggcccgccg gcgggcttct ggtgctcctt gagatcgagc gtgaggtccg
     2761 gactgatcgg cgtccagcgt tcgcctcggt c
//
LOCUS       DASUCJ010000489         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_341541, whole genome shotgun sequence.
ACCESSION   DASUCJ010000489 DASUCJ010000000
VERSION     DASUCJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341541"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="apaG"
                     /locus_tag="VFU90_05835"
     CDS             complement(<1..348)
                     /gene="apaG"
                     /locus_tag="VFU90_05835"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU5479331.1"
                     /translation="MSDDKRYAISVSPKSTYLESQSDPGKGQFVFAYTIIVTNTGNVA
                     AQLVSRHWIITDAEHRVQEVRGAGVVGKQPVLRPGESFEYTSGTSLTTAVGTMHGSYQ
                     MIAEDGTAFDAPIP"
     gene            399..>1045
                     /gene="rpe"
                     /locus_tag="VFU90_05840"
     CDS             399..>1045
                     /gene="rpe"
                     /locus_tag="VFU90_05840"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5479332.1"
                     /translation="MKRNFRIAPSLLSADFARLGDEVRAVIGAGADMLHFDVMDNHYV
                     PNLTIGPLVCEAIRPHSTVPIDVHLMVKPVDRIVPDFAAAGASIISFHPEASEHVDRT
                     IGLIHESGCKAGLVFNPATPLDWLMHVIDSLDLVLVMSVNPGFGGQSFIPHALDKLKA
                     ARRLVDAASERTGREILLEVDGGVKVDNIAAIAAAGADTFVAGSAIFGSADYGAT"
BASE COUNT          164 a          348 c          335 g          198 t
ORIGIN      
        1 cgggatcggc gcgtcgaacg cggtgccgtc ctcggcgatc atctggtagc tgccatgcat
       61 cgtcccgacc gccgtcgtga ggctcgtccc gctcgtgtac tcgaagcttt ccccgggcct
      121 cagcaccggc tgcttgccga ccacccccgc gccacgtacc tcctgcacgc ggtgctcggc
      181 atcggtgatg atccagtgcc ggctgacgag ctgggcggca acgttgccgg tgttggtgac
      241 gatgatcgtg taggcgaaca cgaactgccc cttgccggga tcggactggc tttcgagata
      301 ggtcgatttc ggcgataccg aaatcgcgta ccgcttgtcg tccgacatgc ctgcgttccg
      361 tgcgccattc gccccgattt ctgcgattat agtcggcgat gaagcgcaac ttccggattg
      421 ccccgtcgct cttgtcggcc gatttcgccc gtctcgggga cgaggtgcgc gcggtgatcg
      481 gcgccggtgc cgacatgctg cacttcgacg tgatggacaa ccactacgtg ccaaacctga
      541 cgatcgggcc gctggtgtgc gaagcgatcc ggccgcacag cacggtgccg atcgacgtgc
      601 acctgatggt gaagccggtc gatcgcatcg ttcccgattt cgcggccgcg ggcgcgtcga
      661 tcatcagctt ccatcccgag gcgtcggagc acgtcgatcg cacgatcggg ctcattcacg
      721 aaagcggctg caaggccggg ctcgtcttca acccggcaac acccctcgac tggctgatgc
      781 acgtgatcga ttcgctcgat ctcgtgctcg tcatgtcggt caatccggga tttggcgggc
      841 agtcgttcat tccgcacgcg ctcgacaagc tcaaggcggc gcgcaggctc gtcgacgcgg
      901 cgagcgagcg caccggccgc gagatcctcc tcgaagtcga cgggggagtg aaggtcgaca
      961 acatcgccgc gatcgccgcg gccggcgctg acaccttcgt tgccggaagc gcgatcttcg
     1021 gcagcgccga ctacggcgcg acgat
//
LOCUS       DASUCJ010000490         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_845879, whole genome shotgun sequence.
ACCESSION   DASUCJ010000490 DASUCJ010000000
VERSION     DASUCJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845879"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFU90_05845"
     CDS             <1..212
                     /locus_tag="VFU90_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479333.1"
                     /translation="AERLVLLGVASLVDSPLTRALHWHNGALLLTVIVLIGAGSIGTA
                     VYRTVFVARELRKRERETGEGKHIV"
     gene            complement(228..>1690)
                     /gene="acnA"
                     /locus_tag="VFU90_05850"
     CDS             complement(228..>1690)
                     /gene="acnA"
                     /locus_tag="VFU90_05850"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5479334.1"
                     /translation="SVRMRGQTFTITHGSVVIAAITSCTNTSNPSVMIGAGLLARKAV
                     EKGLTVKPWVKTSLAPGSKVVSDYYRKSGLMASLEKLGFYLAGYGCTTCIGNSGPLPA
                     EIGEALEQNKLVVAAVLSGNRNFEGRVNALTRFNYLASPPLVVAYALAGRMDIDLATE
                     PLGVGADGPVFLRDIWPSSKEVQDTILSSVKSEMFTKEYANVFDGDERWRALPVPTGD
                     LYAWSEDSTYVQNPPFFQGMTLEPPPVRPIRGARVLAMLGDSITTDHISPAGSIPVKS
                     PAGQWLIAHGVKASDFNSYGARRGNHDVMVRGTFANIRLRNELAPGTEGGWTALEAGA
                     TPITIYDASVSYQQAGTPLLIIAGKEYGTGSSRDWAAKGTLLLGVSAVIAESFERIHR
                     SNLVGMGVIPLEFVDGATRQSLGLTGFETYEIEGLSNTLAPRAVLTVRATGSDGTAKP
                     FTVRSRIDTPEEATYYRNGGILQYVLRNLAGGKARR"
BASE COUNT          275 a          573 c          559 g          283 t
ORIGIN      
        1 gggccgagcg tctggtgttg ttgggcgtag cgtcgctggt cgacagcccg ctgacccgcg
       61 cgctccactg gcacaacggc gcgctcctgc tcacggtgat cgtgttgatc ggcgcgggct
      121 cgatcgggac ggcggtgtat cggacggtgt tcgtggcgcg cgagctcagg aagcgggagc
      181 gggagacggg ggaagggaag cacatcgtct agcgggaagc gcgccgccta acggcgtgcc
      241 tttccccctg cgagatttct caacacgtac tgcaaaatcc ccccattcct atagtacgtc
      301 gcttcctccg gcgtatcgat ccgcgagcgc acggtgaacg gcttcgccgt gccatcggat
      361 cccgtggcgc gcacggtgag cacggcccgc ggcgcgagcg tgttcgacag tccctcgatc
      421 tcgtaggtct cgaaccccgt caggcctaac gactggcgcg tggcgccgtc cacgaactcg
      481 agcgggatca cgcccatgcc caccaggttc gaccgatgga tgcgctcgaa cgattcggcg
      541 atgaccgcgg acacgcctaa cagcaaggtg cccttggcgg cccagtcgcg cgacgagccg
      601 gtcccgtact ccttgccggc gatgatcaag agcggcgtgc cggcctgttg gtacgacacc
      661 gacgcatcat agatggtgat cggcgtcgcg ccggcctcga gcgccgtcca gccgccttcc
      721 gtaccgggcg cgagctcgtt gcgcagccgg atgttcgcga acgtgccgcg caccatcacg
      781 tcgtggttgc cgcggcgcgc gccgtacgaa ttgaagtcgg aggccttgac cccgtgcgcg
      841 atcagccact gtcccgcggg actcttcacc ggaatcgagc cggcgggcga gatgtggtcc
      901 gtcgtgatcg agtccccgag catggccagc acgcgcgcgc cgcggatcgg ccgaacgggc
      961 ggcggctcga gcgtcatgcc ctggaagaac ggcggattct ggacgtacgt cgaatcctcc
     1021 gaccacgcgt agagatcgcc ggtcgggacc ggcagcgccc gccaccgctc gtcgccgtcg
     1081 aacacgttcg cgtattcctt ggtgaacatc tcgctcttca ccgacgacag aatcgtgtcc
     1141 tgcacttcct tggacgatgg ccagatgtcg cgcagaaaca cgggcccgtc ggcgccgacg
     1201 cccaacggct cggtagcgag gtcgatgtcc atgcgtcccg ccagcgcgta cgcgacgacc
     1261 agcggcggcg acgccaggta attgaaccgc gtgagggcat tcacccggcc ctcgaagttg
     1321 cgattgccgg acagcactgc cgcgacgacg agcttgttct gctccagcgc ctcgccgatc
     1381 tcggccggca gcggaccgga gttgccgatg cacgtcgtgc acccgtagcc ggcgaggtag
     1441 aagccgagct tctcgagcga cgccatgagg ccggacttcc gatagtaatc cgacacgacc
     1501 ttcgagcccg gcgcgaggct cgtcttgacc cacggcttca ccgttaggcc tttctcgacg
     1561 gccttccgcg ccagcaagcc ggcgccgatc atcaccgacg gattggacgt gttggtgcag
     1621 ctcgtgatgg cggcgatcac gaccgatccg tgggtgatcg tgaacgtctg tccccgcatg
     1681 cgcacgctga
//
LOCUS       DASUCJ010000491         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1059825, whole genome shotgun sequence.
ACCESSION   DASUCJ010000491 DASUCJ010000000
VERSION     DASUCJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059825"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFU90_05855"
     CDS             <1..608
                     /locus_tag="VFU90_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479335.1"
                     /translation="LVKQHLTLVGISGSAAIVSGVALGVWLSRPSMARYAEGAMQAVN
                     MATSIPTLGKLALMMSLLGIGALPAIVGLWIATLLPIIRNTYEGIRAVPEHVLDAARG
                     MGMGPRAILMRVELPNALFVIFAGVRTAMAVNVGTAPIAFLIGGGGLGELIFTGIDLQ
                     EHGMMLAGAIPTALLAVAVDFLFGQAQYWLVPRGVNPLRNS"
     gene            638..>1417
                     /locus_tag="VFU90_05860"
     CDS             638..>1417
                     /locus_tag="VFU90_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5479336.1"
                     /translation="MKRLLGGWLAGVALAILCCGGVSAQMIVVGGKAFTEQQIMTAMT
                     VALLKAKGFTPGRKAGMGSAAVRATLENGQSDVYWEYTGTALGVYHKITDKCPSADAA
                     HQKIKELDAAKGIVWLNMSAVNNTYGFAMNKDDAQKRGIASMSDLAKAINGGAKLTFA
                     SNAEFYARPDGLPGWQKAYGFEFERDNVKRMDTGLTYSALKDRQVDTAVVFATDGRIP
                     AFNFVVLKDDKNYAAPYNLTPVVRKEILDKNPKLGDALNSVA"
BASE COUNT          258 a          438 c          483 g          238 t
ORIGIN      
        1 acctcgtcaa gcagcatctg acgctggtcg ggatctccgg ttcggctgcg atcgtcagcg
       61 gcgtcgcgct cggcgtctgg ctgtcgcggc cgtcgatggc gcgctacgcc gaaggtgcga
      121 tgcaggccgt aaacatggcg acgtcgattc cgacgctcgg caagctcgcg ctgatgatga
      181 gcctcctcgg catcggggcg ctgccagcca tcgtcggcct gtggatcgcg acgctgctgc
      241 cgatcattcg caacacctac gaagggatcc gtgccgtgcc ggagcacgtg ctcgatgcgg
      301 cgcgcggaat ggggatggga ccgcgggcaa tcctgatgcg ggtcgagctg ccgaacgcgc
      361 tcttcgtgat cttcgccggc gtgcgcactg cgatggccgt caacgtcggc acggcaccga
      421 tcgcgttcct gatcggcggt ggcggcctgg gcgagctgat cttcaccggg atcgatttgc
      481 aggaacatgg catgatgctc gccggcgcga tcccgacggc gctgcttgcc gtcgccgtgg
      541 attttttgtt cggtcaggcg cagtattggc tcgtgccgcg cggcgtgaat ccgctgcgga
      601 attcctgaga atttcaacgg gtgaggaggg gagaacgatg aagcggcttt tgggcgggtg
      661 gctcgccggc gtggcgctgg caattctttg ctgcggcggc gtgtccgcgc agatgatcgt
      721 cgtcggcggc aaggcgttca ccgaacagca gatcatgacg gcgatgacgg tcgcgctgct
      781 caaggccaag ggattcacgc cgggccgcaa ggcgggaatg ggcagcgccg ccgtgcgagc
      841 cacgctcgag aacggccaga gcgacgtcta ctgggaatac acgggcactg cgctcggcgt
      901 ctatcacaag atcaccgaca agtgcccgtc cgccgacgcc gcccaccaga agatcaagga
      961 gctcgacgcc gcgaaaggca tcgtctggct caacatgtcg gcggtcaaca acacctacgg
     1021 tttcgcgatg aacaaggacg acgcgcagaa gcgcgggatc gcgtccatga gcgacctcgc
     1081 caaagcgatc aacggcggcg cgaagctcac gttcgcgtcg aacgccgagt tctacgcgcg
     1141 gcccgacggg ctcccgggct ggcagaaggc gtacggattc gagttcgagc gcgacaacgt
     1201 caagcggatg gacacggggc tcacgtattc ggcgctgaag gaccgccagg tcgacaccgc
     1261 cgtcgtgttc gcaaccgacg gccgcattcc ggcattcaac ttcgtcgtgc tgaaggacga
     1321 caagaactac gctgcgccct ataacctgac gcccgtggtg cgcaaggaaa tcctcgacaa
     1381 gaatccgaag ctcggcgatg cgttgaacag cgtcgcc
//
LOCUS       DASUCJ010000492         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1502995, whole genome shotgun sequence.
ACCESSION   DASUCJ010000492 DASUCJ010000000
VERSION     DASUCJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFU90_05865"
     CDS             <1..1290
                     /locus_tag="VFU90_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5479337.1"
                     /translation="VDWAVHMERLSDDDRADHLLGAGAVDEAFVDRIAARIAEFHRTA
                     ATSYEIRRYGDAAVVARNVAENFEQVGDGLERSLSAAEAREVIDYQVAFLREHRARFD
                     ARIAADRIRDGHGDLRLEHVYRRGGEGIVVLDCIEFNDRFRYADVCADIAFLGMDFAA
                     NGRADLAERLLATYARESQDYDLYGVVDFYESYRAFVRGKIASMLARDPGLNDTARTY
                     AETQARRFFRLALSFHRFSLLGPMLVTVGGVIASGKSSLADRLGAMLSAPVIDADRTR
                     KTMAGIGLVERNEDPAFQGAYDPAFSERVYSELFRRASVVLASGRPVVLDASFRTASL
                     REAARQVAATHGVPFRFVECRAPREICLARLASREQGPSVSDGRRAIFDDFVAHYEPV
                     SEMRPAQHAVVDTTKPLDENLGWLRDLLLAWPRGLPG"
     gene            complement(1302..>1664)
                     /locus_tag="VFU90_05870"
     CDS             complement(1302..>1664)
                     /locus_tag="VFU90_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5479338.1"
                     /translation="RVGAVVGPKQIAVRAEVTIRELRGLLAEAPASTVLVVDQDNRLL
                     GTIDSSSLEQAPAAAPTILRMRARTAIAETASVAEAVDCLVHSHSRSVAVVDKDDRAV
                     GVLTDVELLRWFARHGND"
BASE COUNT          267 a          556 c          544 g          297 t
ORIGIN      
        1 gtcgactggg ccgtccacat ggagcgtctc tccgacgacg atcgggccga tcacttgctt
       61 ggtgcgggcg ccgtcgacga ggccttcgtc gatcggatcg ccgcgcgcat cgcggagttt
      121 caccggaccg cggcgacgag ctacgaaatt cgccgctatg gcgacgccgc cgtcgtcgct
      181 cgcaacgtcg ccgagaactt cgaacaggtg ggcgacggcc tcgagcggtc gctctcggcc
      241 gccgaggcgc gcgaagtcat cgactaccaa gtcgcgttct tgcgcgaaca tcgcgcgcga
      301 ttcgacgcgc ggatcgccgc cgatcgcata cgagacggtc acggcgacct acgactcgag
      361 cacgtgtacc ggaggggagg cgagggcatc gtcgttctcg attgcatcga gttcaacgat
      421 cgatttcgct acgcggacgt ctgtgcggac atcgcgtttc tgggaatgga cttcgccgca
      481 aacggacgag ccgatctcgc cgaacgcttg ctcgccacgt atgcgcgcga atcgcaagat
      541 tacgacctct acggggtggt cgatttctac gagagctacc gcgctttcgt gcgcggcaaa
      601 atcgcatcga tgctcgcgcg cgacccgggc ctgaacgaca cggcgcgaac gtacgccgaa
      661 acgcaagcgc gccggttctt ccggctcgcc ctgtcgtttc accggttctc gctgctgggc
      721 ccgatgctcg tcacggtcgg cggggtgatt gcttcgggga agagctccct ggccgatcgg
      781 ctgggcgcca tgctcagcgc accggtgatc gacgccgatc gcacgcgcaa gaccatggcg
      841 ggcatcggtc tcgtcgaacg aaacgaagat cccgcgttcc aaggtgcata cgatcccgct
      901 ttcagcgagc gcgtttattc cgagctcttt cgccgcgcat ccgtggtcct cgcgtcgggc
      961 cgccccgtgg ttctcgatgc gtcgttccgc accgcgtcgc tgcgggaggc ggctcgccag
     1021 gtcgccgcca cccacggtgt tccctttcgg ttcgtggagt gtcgggcgcc tcgcgaaatc
     1081 tgcctcgccc ggctggcgtc gcgcgaacag ggcccttccg tgagcgatgg ccggcgcgcc
     1141 atcttcgacg acttcgttgc ccactacgag ccggtgtccg agatgcgacc ggcgcagcat
     1201 gccgtggtgg atacgacgaa acctctcgac gagaacctcg gatggctgcg ggacttgctt
     1261 ctcgcgtggc cgcgagggct gccgggttga acgacgagcc ttcagtcgtt gccgtgccgg
     1321 gcgaaccacc gcaggagctc gacgtccgtc agaacgccga cggcgcggtc gtccttgtcg
     1381 acgacagcga ccgaacgcga gtgactgtga accaggcaat cgacggcttc tgcgaccgac
     1441 gcggtctcgg caatggcagt tcttgcgcgc atgcgcagaa tcgtcggtgc agccgcgggc
     1501 gcttgttcga gcgacgacga atcgatcgtt ccgaggaggc ggttgtcctg atccacgacc
     1561 agaaccgtgc tcgcaggagc ctcggcaagt aagcctctca actcgcgaat cgtcacctcc
     1621 gcccgcacgg cgatttgctt cggcccgacg acggccccga cgcg
//
LOCUS       DASUCJ010000493         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_372267, whole genome shotgun sequence.
ACCESSION   DASUCJ010000493 DASUCJ010000000
VERSION     DASUCJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372267"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..>1267)
                     /locus_tag="VFU90_05875"
     CDS             complement(188..>1267)
                     /locus_tag="VFU90_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479339.1"
                     /translation="PRAVSPNHHALAERFGIFDRFFTNAEVSAQGHNWSTAAYASDYV
                     EKTVAAQYSDRRPYYDYEGANRNQLTDDDVASPSTGYLWNLAQRAGASFRNYGEFVVS
                     NRELTGRRDLPESYRGDKPFLAAHTDSAYPGFDLSIPDQTRIDRWLEEFRAFETTGDM
                     PALEIVRLPNDHTSGARVGMPTPRAAFADNDLALGRLVEALSRSRFWRSTVVFVVEDD
                     AQNGPDHVDSHRAPFFMISAYSHAGVVHRFVNTTDVIATIADILHLGSLSQFDYYGRP
                     LRGLFADSPDLTPYVAQAPEQRLDDRNPARGPGAAASARLDLRLEDESDDNLFNHVLW
                     EAIKGPAVPYPGTTHHAMAKGALAY"
BASE COUNT          228 a          370 c          460 g          209 t
ORIGIN      
        1 cggccggcgg ccaactcgtg accacgatgt acaacgacac cccgatggcc gcggcaaacg
       61 atacgatcgc gagaagccaa cgggcgggcg tcatgagtgc aaagagggag tcgctgggtt
      121 gcggacggtg acgacgatga cgagcggtgc gccggaatcg agaaagggta cagcgtgcag
      181 cgtgacttca gtaggccagc gcgccctttg ccatcgcgtg gtgcgtcgtg ccgggatacg
      241 gcacggccgg ccccttgatc gcctcccaca gaacgtggtt gaagaggttg tcgtccgatt
      301 cgtcttccag ccgaaggtcg agccgggcgg atgccgcggc gccgggaccg cgtgccggat
      361 tgcgatcatc gagccgctgc tccggcgcct gtgctacgta cggcgtcaga tccggcgaat
      421 cggcgaagag tccccgcagc gggcggccgt aatagtcgaa ttgcgacagg gaaccgaggt
      481 ggagaatgtc ggcgatggtg gcaatcacgt ccgtggtgtt cacgaatcgg tgcacgactc
      541 cggcatgact gtaggcgctg atcatgaaaa acggcgcgcg gtgcgaatcc acatggtcgg
      601 gtccgttctg cgcgtcgtct tccacgacga acacaactgt actgcgccag aagcgcgagc
      661 gcgagagggc ctcgacgaga cggcccaacg ccaggtcgtt gtcggcaaac gcggcacgag
      721 gcgtgggcat gccgacccgg gcgccggagg tatggtcgtt gggcagacgg acgatctcca
      781 acgccggcat gtcgccggtc gtctcgaacg cgcgaaactc ctcgagccat cgatcgatgc
      841 gcgtctgatc ggggatcgag aggtcgaatc cgggatacgc ggaatcggtg tgcgcggcga
      901 gaaacggttt gtcgccgcgg taggactcgg gcagatcccg gcggccggtg agctcgcgat
      961 tgctcaccac gaactcgccg taatttctga atgaggcccc ggctcgttgg gcgagattcc
     1021 agaggtagcc ggtggacggc gaggccacgt cgtcgtcggt gagctggttg cgattcgcgc
     1081 cctcgtagtc gtagtagggc cggcggtcgg agtactgcgc ggcgaccgtc ttctcgacgt
     1141 agtcgctcgc gtacgcggcg gtcgaccagt tgtgcccttg cgcgctcacc tcggcgttcg
     1201 tgaagaagcg gtcgaagatg ccgaagcgct cggccagggc gtgatggtta ggcgaaacgg
     1261 cgcgcgg
//
LOCUS       DASUCJ010000494         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1380809, whole genome shotgun sequence.
ACCESSION   DASUCJ010000494 DASUCJ010000000
VERSION     DASUCJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380809"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..629
                     /locus_tag="VFU90_05880"
     CDS             153..629
                     /locus_tag="VFU90_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479340.1"
                     /translation="MPIFALAAMLVAQSLLTLPIDGIQCNATEGVAEHIHTYLQIFDR
                     GKPVPVPADVGIPPGAGCLYWVHTHAAGGYIHIESPVIRTFTLGQFFDVWDRDLSWTQ
                     AAGAKAPHGARVSVWVNGKAWHGSNPRAIPLRDHETIVIQSAAPFAKPSPADWANL"
     gene            643..>1599
                     /locus_tag="VFU90_05885"
     CDS             643..>1599
                     /locus_tag="VFU90_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEU5479341.1"
                     /translation="MSGVELDGHHLTLEDLEAVVDGVPVAIAPPARERVARARAFVDE
                     RFAGGDAIYGVTTGFGRLANVPVDPADAEQLQLNLVRSHAAGMGAPLQPRLVRAAGVL
                     RANSLSAGHSGIRPATLELIVELLNRGVTPVVPCQGSVGASGDLAPLAHMTLTLIGEG
                     EAYYRGERLPSAVALERAGLKPVVLGAKEGLALINGTQVMTGIAGLGVLRAERLAAVA
                     DVIGAMSLEAYHGTERVFDRRLNELRPHPGQLRVAANLRALLAGSEIVKSHAECGRVQ
                     DPYSFRCIPVVHGAVRDSIAHVRRVTETEINSVTDNPLVFPED"
BASE COUNT          259 a          524 c          553 g          263 t
ORIGIN      
        1 ggaatctgtt ccgaagttcg agcatcgtag catacgaaaa cggcaacgac aagcgctcgt
       61 tcgccggaat tcacagactc tccacaattt ttttgcgctt atgcaccgtg gccggttggg
      121 ttatccacgg tggttgacga acgtgcgctc gcatgccaat tttcgcactc gccgcgatgc
      181 tcgtagcgca gagcctgctc acgctgccga tcgacggaat ccaatgcaac gcgaccgaag
      241 gcgtcgccga acacatccac acgtatttgc aaattttcga ccgcggaaaa ccggtgccgg
      301 tgcccgccga cgtcggcatc ccgccgggag ccggttgcct ctactgggtg cacacgcacg
      361 cggcgggcgg ctacatccac atcgagtcgc cggtgattcg gaccttcacg ctcggccagt
      421 ttttcgacgt ttgggatcgg gatttgagct ggacgcaagc ggccggtgcc aaagcgccgc
      481 acggcgcgcg cgtctccgtg tgggtcaacg gcaaagcgtg gcacgggtcg aacccgcggg
      541 cgatcccgct gcgcgatcac gaaacgatcg tgatccaaag tgccgcgccg tttgcgaaac
      601 cgtcgccggc cgactgggcg aacctctagg actgctcgaa tcgtgagcgg cgtcgaactc
      661 gacgggcacc atctcaccct cgaagatctc gaagcggtgg tcgacggcgt gccggtggcg
      721 atcgcgccgc cggcgcgcga gcgcgtcgcc cgggcccggg cgttcgtcga cgagcggttt
      781 gccggcggcg acgcaatcta cggcgtcacg acgggctttg ggcggctggc caacgttccg
      841 gtcgatccgg cggacgccga gcaactgcag ctcaacctgg tgcgcagcca cgccgccggg
      901 atgggcgcgc cgctgcagcc gcggctggtg cgcgcggcgg gcgtgctgcg cgccaactcg
      961 ctgtcggcgg gtcactcggg catccggccg gcgacgctcg agctgatcgt cgagctgctc
     1021 aatcgcggcg tcacgccggt ggtgccgtgc cagggatcgg tcggcgcaag cggcgatctc
     1081 gcgccgctcg cccacatgac gctgacgctg atcggcgagg gcgaggcgta ctatcgcggc
     1141 gagcggcttc cgagcgccgt cgcgctcgaa cgtgccgggc tcaaaccggt cgtgctcggc
     1201 gccaaggaag ggctggcgct gatcaacggg acgcaggtga tgaccggcat cgcggggctc
     1261 ggcgtgctgc gagccgagcg tttagccgca gtggccgacg tgatcggtgc gatgtcgctg
     1321 gaagcctatc acgggaccga gcgcgtgttc gaccggcggc tcaacgagct gcgtccgcat
     1381 cccggacagc tgcgcgtggc cgccaacctg cgcgcgctgc tggccggttc ggagatcgtc
     1441 aaatcgcacg cggagtgcgg gcgggtgcaa gacccgtact cgttccgctg cattccggtc
     1501 gtgcacggtg cggtccgcga ttcgatcgca cacgtgcgcc gggtgacgga aaccgaaatc
     1561 aactcggtga ccgacaaccc gctggtcttt ccggaggac
//
LOCUS       DASUCJ010000495         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_952714, whole genome shotgun sequence.
ACCESSION   DASUCJ010000495 DASUCJ010000000
VERSION     DASUCJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952714"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VFU90_05890"
     CDS             <1..1061
                     /locus_tag="VFU90_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479342.1"
                     /translation="RWQSFKNDLPSVSVRDIRIQPRADDLVIATHGRGVYIMDDVRPV
                     QELQRAVSAGSWLFAPRAAREWTLHSNDEGTYTNYAADNPPYGVTVTFYQREPQKGNP
                     ALEILDSSGHVIRSISGTHKIGGKDVPYITNKVGLNRYTWDFTVNGPVRWNGAPQEFL
                     KGPTSGPGVVPGKYAVRMRLSGRTYVEHFAVEPDPRSRFTMAEYRASFDEAMRQMARL
                     SQLDTILNTLDDLKKALADASDAAKKANNSALAGKLADAETARATLFDSLAANVRGEG
                     TEDETKLHEDVTGAYFTAQGLITPAAMDFIERVDVTYRAGIARYNAFVTGVLPGVNAA
                     LRGAGQKALPVAQPASAK"
     gene            1058..>1368
                     /locus_tag="VFU90_05895"
     CDS             1058..>1368
                     /locus_tag="VFU90_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479343.1"
                     /translation="MKALVAAAALAGSAALVLPSAARAADYDNLTWRSIGPAVSGGRI
                     AAVAGTPQDDRLYYVGTAGGGVWKSADGGATWQPVFDKQPVAAIGAVAIDPHNAGVVY
                     VG"
BASE COUNT          247 a          450 c          466 g          205 t
ORIGIN      
        1 cgcgctggca atcgttcaag aacgatctgc ccagcgtctc ggtgcgcgac attcgcattc
       61 agccgcgcgc cgacgatctc gtgatcgcga cgcacggccg gggcgtgtat atcatggacg
      121 acgttcggcc ggtccaagag ctgcagcgcg cggtgtcggc cggctcttgg ctgttcgcgc
      181 cgcgcgcggc gcgcgagtgg accctgcatt ccaacgatga gggaacctac acgaactacg
      241 cggccgacaa cccgccgtac ggcgtgacgg tcacgttcta ccagcgcgag ccgcagaagg
      301 gcaacccggc cctggaaatt ctcgattcga gcgggcacgt gatccgaagc atttcgggaa
      361 cgcacaaaat cggcggcaaa gacgtgccgt acattaccaa caaggtaggc ctcaaccgct
      421 acacgtggga ttttacggtc aacgggccgg tgcgctggaa cggcgcaccg caggagttcc
      481 tcaaggggcc gacctccggg ccgggcgtcg tgcccgggaa atacgccgtg cggatgcgtc
      541 tttcgggccg cacctacgtc gagcatttcg ccgtcgaacc ggatccgcgc tcgcgcttca
      601 cgatggccga ataccgagcg agcttcgacg aagcgatgcg gcagatggcg cgtctctcgc
      661 agctcgatac gattctcaac acgctcgacg atctgaagaa agcgctggcc gatgcgtcgg
      721 atgctgccaa gaaggccaat aactcggcgc tcgccgggaa gctggcggac gccgagaccg
      781 cccgcgcgac gctcttcgac tcgctggccg ccaacgtgcg cggcgaaggc accgaagacg
      841 aaaccaaact gcacgaagac gtgaccggcg cctactttac ggcgcaaggc ttgatcacgc
      901 cggcggcgat ggatttcatc gagcgcgtcg acgtcacgta tcgcgccggc atcgcacgct
      961 acaacgcgtt cgttacgggc gttctgccgg gcgttaacgc ggcgttgcgc ggcgccggcc
     1021 agaaggcgct tccggtggcc caaccagcca gcgcaaaatg aaggcgctcg ttgcggcggc
     1081 ggcgctggcg ggctcggcag cgctcgtgct gccgtcggcc gcgcgagcgg ccgactacga
     1141 taacctgacg tggcgttcga tcggaccggc ggtatccggc ggacgaatcg cggcggttgc
     1201 cgggaccccg caggacgacc gcctctacta cgtgggaacg gccggcggcg gcgtttggaa
     1261 gagcgccgac ggcggcgcga cgtggcagcc ggtcttcgac aagcagccgg ttgcggccat
     1321 cggcgcggtc gcgatcgacc cgcacaacgc cggcgtcgtg tacgtcgg
//
LOCUS       DASUCJ010000496         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_158122, whole genome shotgun sequence.
ACCESSION   DASUCJ010000496 DASUCJ010000000
VERSION     DASUCJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158122"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1078
                     /locus_tag="VFU90_05900"
     CDS             <1..>1078
                     /locus_tag="VFU90_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479344.1"
                     /translation="VFPLPQTIAEVQMIRGGSSLLYGPEPPPAINLVSAKPSTEPMAG
                     YTENVVGNDGLFATFNKVSGTQGDWDYLVDAHYRTTDGQRDNGDNKLRGADLHIGYRP
                     DETSYTALDFHAYDLDTGNAGLLTYPQYQANADQTTTPYDRLWTQRYVLSLAHDRHLD
                     ENTELVAKVWAGYQDQASRSQDRGAAPTTTTLQDEQFRFTGIDARLVHHWGRGNAFTA
                     GTTAYHSDAPFRQWDDFGLASDQYDRPGVACATPASTDCAKLRQARSTNYAAVFAENV
                     FRLPHEWHVVPSVRLEREQVNIDETIKPPALNRGLVDRSVSHNVPLFGIGIGNDFGHL
                     NESYFNVSQGWRPVRYFDIGSPFGS"
BASE COUNT          200 a          378 c          326 g          174 t
ORIGIN      
        1 cgtgttcccg ctgccacaga ccatcgccga agtgcagatg atccgcggcg gatcgtcgct
       61 gctgtacgga ccggaaccgc cgccggcgat caacctggtc agcgccaagc cgtcgaccga
      121 gccgatggcc ggctacaccg agaacgtggt cggcaacgac ggcctgttcg ccacattcaa
      181 caaggtatcg ggcacgcagg gcgactggga ttacctcgtc gacgcgcatt accgcacgac
      241 ggacggccag cgcgacaacg gcgacaacaa gctgcgcggc gcagacctgc acatcggcta
      301 tcgtccggat gaaacctctt acacggcgct ggatttccac gcctacgacc tcgataccgg
      361 caatgccggc ctgctcacct atccgcagta ccaggccaat gccgatcaga cgacgacgcc
      421 gtacgaccgg ctatggacgc agcgttacgt gctgagcctt gcccatgatc ggcacctgga
      481 tgagaacacc gaactcgtcg ccaaggtctg ggctggctat caggatcagg cctcgcgttc
      541 gcaggatcgc ggcgcggcgc cgaccacgac gacgctgcag gatgagcagt tccgcttcac
      601 cggcatcgac gcgcgcctcg tccaccattg gggccgcggc aacgcgttca ccgccggcac
      661 cacggcgtac cactccgacg cgccattccg gcaatgggac gatttcgggc tcgcctccga
      721 tcaatacgac cgcccgggcg ttgcctgcgc gacgccggca tccaccgatt gcgcgaagct
      781 gcgccaggcg cgttcgacga attacgccgc cgtgttcgcc gagaacgtgt tccgcctgcc
      841 gcacgaatgg catgtcgtgc cgtccgtgcg cctggaacgc gagcaggtga acatcgacga
      901 gacgatcaag ccgcccgcgc tcaaccgcgg cctcgtcgat cgctcggtca gccacaacgt
      961 tccgctgttc ggcattggca tcggcaacga tttcgggcat ctgaacgaga gctacttcaa
     1021 cgtctcgcag ggctggcgtc cggtgcgcta tttcgacatc ggctcgccgt tcggctcg
//
LOCUS       DASUCJ010000497         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_280382, whole genome shotgun sequence.
ACCESSION   DASUCJ010000497 DASUCJ010000000
VERSION     DASUCJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280382"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFU90_05905"
     CDS             <1..483
                     /locus_tag="VFU90_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479345.1"
                     /translation="KQNCTHQFDAACYAITHAAWGRDERVYDVEVPMRDWDDDGASHV
                     RLWVDGALRLEWNIKWDRIENASAPFDAAPWKGGFMRWADATLGEEDAENAITLRRAS
                     DIGMGRSMDLDGIPVATQLPQIMAGICHTMQPGVVEVAFRHVGSIRDFAQHPERLLAD
                     "
     gene            complement(480..1082)
                     /locus_tag="VFU90_05910"
     CDS             complement(480..1082)
                     /locus_tag="VFU90_05910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5479346.1"
                     /translation="MRLGTHVFNIGLRHPFATARGVQTVDVLSGGRFEFGIGASWLEE
                     EWNATQLDFATRGRRVDEAVEVCKRLWTEETVSHHGEFFAFDEAVFEPKPVQKPWPPI
                     LVGGESKAALRRAARLGDGWIGVGHSFDSAKAQIVLLRSYLNESGRGDEGFQIVLGGP
                     VTSRADVTQWEDLGVTRLIVSPWKRSPEAVESLQRFAKFL"
BASE COUNT          213 a          361 c          345 g          186 t
ORIGIN      
        1 aagcagaatt gcacgcacca gttcgacgcc gcttgctacg cgatcacgca cgcggcatgg
       61 ggacgcgacg aacgcgtcta cgacgtcgag gtgccgatgc gcgattggga cgacgacgga
      121 gcatcgcatg tacggctctg ggtcgacggc gcactgcgac tcgaatggaa catcaagtgg
      181 gaccgcatcg agaatgcgtc ggcgccgttc gacgcggcgc cgtggaaggg cgggttcatg
      241 cgttgggccg acgcgacgct cggcgaggaa gacgccgaga atgcgatcac gctgcgccgc
      301 gcttccgaca tcgggatggg ccgcagtatg gatctcgacg gcattcccgt cgcgacccag
      361 ctgccacaga tcatggccgg gatctgccac accatgcagc ccggcgtcgt ggaagttgcg
      421 ttccggcacg tcggttcgat ccgcgacttc gcgcagcacc ccgaacgcct gctcgccgat
      481 taaaggaatt tcgcgaagcg ctgcaacgat tcgaccgctt cgggcgatcg tttccacggc
      541 gacacgatga gtcgagtcac gccgaggtct tcccattgcg tgacgtcggc gcgcgacgtg
      601 actggtccgc cgagcacaat ctgaaagcct tcatcgccgc ggcctgattc attgaggtac
      661 gaacgcagca gcacaatctg tgccttcgcc gaatcaaatg aatgccccac gccgatccaa
      721 ccgtcgccga gtcgagcggc gcgccgcaat gccgccttcg actcgccgcc aaccaggatg
      781 ggcggccaag gtttctggac gggcttcggt tcgaacacgg cttcgtcgaa cgcgaagaac
      841 tcgccgtgat gcgacacggt ttcttcggtc cacaagcgct tgcagacttc gaccgcttcg
      901 tcgacgcgcc ggccgcgcgt tgcaaaatcg agctgagtcg cgttccattc ttcttcgagc
      961 cacgatgcgc cgatgccaaa ctcgaagcgc cctcccgaca gcacatcgac cgtttgcaca
     1021 ccgcgcgcag tcgcaaatgg atgacgcagt ccgatgttga agacgtgcgt gccgaggcgc
     1081 acgcgctcgg tgcgccccgc gagaa
//
LOCUS       DASUCJ010000498         1387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_555557, whole genome shotgun sequence.
ACCESSION   DASUCJ010000498 DASUCJ010000000
VERSION     DASUCJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555557"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFU90_05915"
     CDS             <1..321
                     /locus_tag="VFU90_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU5479347.1"
                     /translation="GDALAVETGGGQTVYGTSHGRATAGEACLSVRAVHFTLEKERPP
                     GERNVWSATVHRSVFLGDLTQVHVLWGDRELVVRQSGMQLWEDGQPVWLSVDPRHCVL
                     LEPN"
     gene            complement(328..>1387)
                     /locus_tag="VFU90_05920"
     CDS             complement(328..>1387)
                     /locus_tag="VFU90_05920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5479348.1"
                     /translation="PVNATARADPAPGRMRLSAMKLSVFSVQDHYPAQSRTVQQLYGE
                     VIAQAELADALGYDTFWVAEHHFHEYGVVPNPAVMLSSLAQRTKRLRLGTAISILTFH
                     NPLTVAESYAMVDVLSGGRLVYGVGSGYLSHEFAGYRIDPSEKRDRFDENLAIVRRLL
                     AGERVTADGKFGKVDAVALNVVPVQREVPIYVAVLRREAAFHIGKQGNNLMCVPYASL
                     EHFDQIGDLIAEYRRGKAEAGVAPSGDDAVVTLHTHVAESDDAARRNAEKAFNLYVDT
                     RLYARKSTYDDAMRNGLHLFGSVETVADKLVALHKAGVRHVSTLQNFGLMPQPLVRAS
                     MERLMREVAPRVVARTGA"
BASE COUNT          230 a          443 c          482 g          232 t
ORIGIN      
        1 ggcgatgcgc tcgcggtcga gaccggaggc gggcaaaccg tttacggcac gtcgcacggg
       61 cgcgcgacag ctggcgaggc gtgcctgtcg gtgcgcgcgg tgcacttcac gctcgaaaaa
      121 gagcggccgc ccggcgagcg caacgtctgg tcagctaccg tgcaccggtc ggtgttcctc
      181 ggcgacctga cgcaggtgca cgtactttgg ggcgaccgcg aacttgtcgt gcggcaatcg
      241 ggaatgcagc tctgggagga cggccagccg gtgtggctgt cggtcgatcc gcgccactgc
      301 gtgctgctgg agccaaacta gcccgcgtta ggccccggtg cgcgcaacga cgcgcggcgc
      361 cacttcgcgc atgagccgtt ccatcgacgc gcgaacgagc ggctgcggca tcaggccgaa
      421 gttttgcagc gtcgagacgt ggcgcacgcc ggccttgtgc aaggcgacca gcttgtcggc
      481 gaccgtttcg accgagccga acaggtgcag gccgttcctc atcgcatcgt cataggtcga
      541 cttgcgcgcg tagaggcgcg tatcgacata gaggttgaac gccttttcgg cattgcgccg
      601 cgcggcgtcg tccgattcgg cgacgtgagt gtgcagcgtc acgaccgcat cgtcgccgga
      661 gggcgcgacg ccggcttccg ccttgccgcg gcgatattcg gcgatcagat cgccgatctg
      721 gtcgaaatgc tccagcgacg cgtaaggcac gcacatcaga ttgttgccct gcttgccgat
      781 gtggaaggcg gcctcgcgcc gtagaacggc gacatagatc gggacctcgc gctggaccgg
      841 cacgacattg agcgcgacgg cgtccacctt gccgaacttg ccgtccgccg tgacgcgctc
      901 gccggccagg agccgccgca cgatcgcgag gttctcgtcg aagcggtcgc gcttttcgga
      961 tggatcgatc cggtagccgg caaattcgtg agacaggtag cctgagccga cgccgtagac
     1021 gagccgcccg ccggacagca cgtcgaccat ggcatagctt tccgcgacgg tgagcggatt
     1081 gtggaaggtg aggatcgaaa ttgcggtgcc gagacgcagg cgcttcgtgc gctgcgccag
     1141 gctcgacagc atcaccgccg ggttgggcac gaccccgtat tcgtgaaagt gatgctcggc
     1201 cacccagaac gtgtcgtagc cgagcgcgtc ggcaagctcg gcctgggcga tgacctcgcc
     1261 atagagctgc tgcaccgtgc gcgactgggc ggggtaatgg tcctgcaccg agaagaccga
     1321 aagtttcatc gcgctcaacc gcattctccc cggggccggg tccgctcgtg ccgtcgcgtt
     1381 gacaggc
//
LOCUS       DASUCJ010000499         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_571075, whole genome shotgun sequence.
ACCESSION   DASUCJ010000499 DASUCJ010000000
VERSION     DASUCJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571075"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFU90_05925"
     CDS             complement(<1..1056)
                     /locus_tag="VFU90_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5479349.1"
                     /translation="MRTLTPLARITATLLLTAFLVVALAIAVQGAFQTRASIADTFSR
                     QARIQAAQITLEELLRLQIVEENALRGYAQTHDSFYVDQYRTAAASYDEKVRSIRSAL
                     EPERLVRAEQLLTEYARIQSQWRDEIAVPMVRHPNQGLAELDKRSKVFSDYENRIVSA
                     IRAELQAANEAFARSTQAQLARTSYVRAFWLLLFGLLAILFNAYRSRLNQELEEERTT
                     TEILEQAFRSESEQVPNLDVGTAYRSASRHAAVGGDVFDLYRLSDRLALLTIADVSGK
                     GIDAAVLTAFIKFEIRSIALRLNEPGAILAEFNHALSRVVRNPYLFVSMFVGVLDTET
                     SALRYASAGHDSAFLRRS"
     gene            1112..2461
                     /locus_tag="VFU90_05930"
     CDS             1112..2461
                     /locus_tag="VFU90_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479350.1"
                     /translation="MQTRVNPGIFKSYDVRGIYPAELNDDVAYAIGRSFADLLGARRI
                     AVGRDMRPSGEKLFEAFARGATESGADVVDVGMVSTDALYFAVGKYGLDGGVMITASH
                     NPAQYNGMKFTRSQAQAISLDTGLAEIRDRLMAGDLPPKAARAGTVTHRNILDDFAAH
                     CLSFVDPATIRPFKIAVDAGNGMAGETAPHVFARLNCEIVPLYFELDGSFPNHPASPI
                     EPENMADVQAAVKKHGCDLGVAFDGDADRMFVVDEKGALVDGSTVTALVALNTLKRYP
                     GAKILYNLICSRSVPELIEKAGGVPIRSKVGHSIIKAVMREQDVVFGGEHSGHFYFRD
                     NWYADSGMIALLQCLELFGKAGRPVSEVIAPIDTRFRSGEINSQVRDVAAKLAQLEAH
                     YRDAAIDHLDGITISYPDWWMNVRPSNTEPLLRLNVEGDTRELMERHRDEALALIRE"
     gene            2454..>2738
                     /locus_tag="VFU90_05935"
     CDS             2454..>2738
                     /locus_tag="VFU90_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479351.1"
                     /translation="MSRVDADAMRRYERTRDDLVARLSALEPLLEGERRASVRDARLL
                     LERGKFVLAVVGEFSSGKSYLLNALLGKFRYEQTGSSREVAGLLATDINPS"
BASE COUNT          497 a          873 c          885 g          483 t
ORIGIN      
        1 tgaacgtcgc aggaaggccg aatcgtgccc cgcgctcgca tatcgcagcg cggacgtttc
       61 ggtgtcgagc acgcccacga acatcgagac gaacagatag gggttgcgca ccacgcggga
      121 gagcgcatgg ttgaattccg ccaggatggc gcccggctcg ttgagccgca gcgcgatcga
      181 gcggatttcg aatttgataa aggcggtcag caccgccgcg tcgatgccct tgccgctgac
      241 gtcggcgatg gtcagcagcg cgagccgatc ggaaagccgg tacaggtcga agacgtcgcc
      301 gccgacggcc gcatgcctgc ttgccgaacg gtaggcggtg cccacgtcca gattcggcac
      361 ttgctccgat tcgctgcgaa aggcttgttc gagaatctcg gtagtggtgc gttcttcctc
      421 gagctcttga ttgagccggc tgcgataggc gttgaacaga atcgccagca gcccgaacag
      481 caagagccaa aaagcgcgta cgtacgacgt gcgcgcgagc tgcgcttggg tgctgcgcgc
      541 gaacgcttcg ttggccgcct gcagttcggc gcggatcgcc gacacgatcc ggttttcgta
      601 gtcggagaaa actttcgaac gcttgtccag ctcggccaag ccttggttcg gatggcgtac
      661 catcggcacg gcgatctcgt cgcgccactg gctttggatg cgcgcgtatt cggtgagcag
      721 ctgctccgca cgcaccaggc gctcgggttc gagcgcggag cggatcgagc gcaccttttc
      781 gtcgtacgag gcggcggcgg tacgatattg atcgacgtag aacgaatcgt gcgtctgcgc
      841 gtagccgcgc agcgcgttct cctcgacgat ctgcagccgc aagagttcct cgagcgtgat
      901 ttgcgcggcc tgaattcgcg cctgacgcga gaacgtatcc gcgatgctcg cgcgggtctg
      961 aaacgcaccc tgaacggcga tggcgagcgc caccaccaga aaggcggtca gcagcagcgt
     1021 cgcggtgatg cgcgcgagcg gcgtgagagt cctcatcgag gcgcccgcgg gctaggtttt
     1081 gctatgggtc cctccgaaca cgaagcctgg catgcaaacc cgagtaaacc ctggaatttt
     1141 caagtcgtac gacgtccgcg gaatttatcc ggccgaattg aacgacgacg tcgcgtacgc
     1201 catcggccgc agcttcgcgg atctgctcgg agcgcgccgg atcgcggtgg gccgcgacat
     1261 gcgtccctcg ggggaaaagc tcttcgaggc gttcgctcgc ggcgccaccg aatcgggcgc
     1321 cgacgtggtc gacgtcggca tggtttccac cgacgcgctg tattttgcgg tcgggaagta
     1381 cggattggac ggcggcgtga tgatcaccgc ctcgcacaat cccgcgcagt acaacggcat
     1441 gaaatttacg cgctcccaag cgcaggccat ctcgctcgat accgggctgg cggaaattcg
     1501 cgaccgcctg atggcgggcg atctcccgcc caaagcggcg cgcgccggca ccgtcacgca
     1561 ccgcaacatc ctcgacgact tcgcagcgca ctgcttgtcg ttcgtcgacc ccgctacaat
     1621 ccggcctttc aagattgccg tcgacgccgg caacggaatg gccggcgaaa cggcgccgca
     1681 cgtcttcgcg cggttgaact gcgagatcgt tccgctctat ttcgagctcg acgggagctt
     1741 cccgaatcat cccgccagcc cgatcgaacc cgaaaacatg gccgacgtcc aagccgccgt
     1801 caagaagcac ggctgcgatt tgggcgtcgc gttcgacggc gacgccgacc ggatgttcgt
     1861 cgtcgacgaa aagggcgcgc tggttgacgg cagcacggta acggcgctgg tggcactcaa
     1921 cacgctcaaa cggtatcccg gtgcgaagat cctctacaat ctcatctgca gccgcagcgt
     1981 gcccgagctg atcgagaagg ccggcggcgt tccgattcgc tccaaggtcg ggcattccat
     2041 catcaaggcg gtgatgcgcg agcaagacgt cgtcttcggc ggcgagcaca gcgggcattt
     2101 ctacttccgc gacaactggt acgccgattc cggcatgatc gcgctcctgc agtgtctgga
     2161 gctgttcggc aaggccggcc gcccggtcag cgaggtcatc gctccgatcg acacgcggtt
     2221 ccgctccggc gagatcaaca gccaagttcg cgacgttgcc gccaagctgg cgcagctgga
     2281 agcgcactat cgcgacgccg cgatcgacca tctcgacggc atcacgattt cctatccgga
     2341 ttggtggatg aacgtccgcc cttcgaacac cgaaccgctg ctgcgcctca acgtcgaggg
     2401 cgacacgcgg gaattgatgg aacgccaccg cgacgaggcg ctagcgctca ttcgtgagta
     2461 gggtcgacgc cgatgcgatg cgtcggtacg aacggacgcg cgacgatctc gtcgcgcgtc
     2521 tctcggcgct cgaaccgctg ctcgaaggcg agcggcgggc gtcggtgcgc gacgcgcgcc
     2581 tgttgctcga gcgcggcaag ttcgtgctcg ccgtggtcgg cgagttctcc agcggcaagt
     2641 cttacctgct caacgcgctg ctcggaaagt tccgttacga gcaaaccgga tcgtcgcgcg
     2701 aggtcgccgg gttgctcgcg accgacatca atccgtcg
//
LOCUS       DASUCJ010000500         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_173394, whole genome shotgun sequence.
ACCESSION   DASUCJ010000500 DASUCJ010000000
VERSION     DASUCJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173394"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFU90_05940"
     CDS             <1..596
                     /locus_tag="VFU90_05940"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479352.1"
                     /translation="WLGKATERLFLPLLQLVMPEIVDMNLPVEGGFHNLAIVSIRKSY
                     PGHAKKVMSALWGLGHMMMLTRVLIVVDADVDVQDTRAVAWFVLNNLAPERDVVMMPG
                     PVDDLDHGSYSIAYGTKAGIDATRKDAAEGYTREWPPDTVMEQSVRERVAERWHEYGL
                     DAVARALRESDGWSGQGPQAYAKLVAGAAPPGEAGAR"
     gene            593..1462
                     /locus_tag="VFU90_05945"
     CDS             593..1462
                     /locus_tag="VFU90_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEU5479353.1"
                     /translation="MKAVLFLKELRIEHTLFALPFAYVGALLAARGFPSWAALGWITL
                     AVVGARTAAMAANRYLDRDIDARNPRTARRAVASGALAPATMLWAVAAGLIVLLGAAW
                     MLNPLCVELMPVAALALLIYPLCKRFTWLAHFVLGAVDALAPLGAYIGIAGRADWPAV
                     LLFLAVTLWVAGFDIIYALMDLPIDRAQGIRSLPARFGETSAHALPLALHLVMLGLLA
                     WAGWTAHAGWLYYAGVAAAAVLVAYERRLLDTAENPFALNDRIFVSNMTFSVVFLGTT
                     AANYLAPAARGGG"
     gene            1459..2625
                     /locus_tag="VFU90_05950"
     CDS             1459..2625
                     /locus_tag="VFU90_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479354.1"
                     /translation="MRRRRFLTGLAAGAAATAASARAQVPLPNQQFLQQLTVGVNVTL
                     SGPLEKYGADVVRGAQAAVDETNRFTTPINHVWGLRAFDDRNDPALAATNASVAAADP
                     SVVGIVGNLTAPMTLAALSRYANVNFAVVVPTVTADAITARGFHNVFRLPANDSAAGR
                     LFAGAVLPDKKSAPALAVAFDGDYGFDVARGFVAQAKADRHPADVLLFPTNGVDPAAA
                     ARTVLDRSPGYVFLCGKTAALGPVAAALRLAGYTGEFGASDGFYNGETLTSYAKVLDG
                     AFVSASLPPLERVPSIMALLTDFQREVGAITAFSAFGYAAAQVLIAAAQRSGATTRFS
                     LLPAMQRQSTFITLVGQYSFSVTGDPLIPNVYLYTVTKDGFKFARPAIRTGFVL"
     gene            complement(2646..2798)
                     /locus_tag="VFU90_05955"
     CDS             complement(2646..2798)
                     /locus_tag="VFU90_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479355.1"
                     /translation="MEILGTNFVLSLGAWRLRFVLAIEDTDAQPARKAAPPHRMRVVP
                     EDEYVR"
     gene            complement(2803..3837)
                     /locus_tag="VFU90_05960"
     CDS             complement(2803..3837)
                     /locus_tag="VFU90_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479356.1"
                     /translation="MTYVEWLRVRAALKWTVVVLGALVLIALVLRVSLLAFGKDDALS
                     FVNGMETDANSRVAHSTLPDGTRRTTVDNAKEDVHIVIDDSGYQGKRITIYESSSHGK
                     RHAPKTIEMGSLNVQSTAQGQETVTTIETNQPESFAYYAAIATFIALIVATVLGAPFA
                     RENDGHLEIALTKPIDRSALALQTIGVDLLGILAAWAVAVIALVVGHSIFEAPHFIFR
                     PVDATIVVLGLVGVTAWYAMLCAATASMRRAFGVVLGLSWPVSALVVLFARLPLQGSA
                     LGQVVHAIATPFAWINPFFYLHFGPAVTVDGQPKGSLAVDPLHELPALAILALIYLAL
                     AVWQWRRVEA"
     gene            complement(3834..4748)
                     /locus_tag="VFU90_05965"
     CDS             complement(3834..4748)
                     /locus_tag="VFU90_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5479357.1"
                     /translation="MSTIQISHLRKTYGPLAAVDDVSLEIPSGTVFGLLGPNGAGKTT
                     AFKCMLGLARPSAGQILYDGKPLEPATFHDVGYVPERSVLYEWMTVAEHVEMNRRAFA
                     NFDPVRAAELLRQFSIDPRKRARALSKGMRTAVMVALAFARNAAILILDEPTSGLDPI
                     NQRHVLSLIINESARGNTVIFSSHQVGQVERAAERIGVLDRGRLILDGAIDDLKADRK
                     IVEGIFPGVDYALDGIAGDPRVARLDRTERIVRLLVLRDADEIAARLTNAGASGVRVL
                     DLNLEDIFLYAVSPSDATADVVAQGSPS"
BASE COUNT          803 a         1647 c         1620 g          816 t
ORIGIN      
        1 cttggctcgg gaaggccacc gagcgtctct ttcttccgtt gctgcagctg gtgatgcccg
       61 agatcgtcga catgaatctc ccggtggagg gcgggtttca caacctcgca atcgtttcga
      121 tccgcaagtc ctaccccggg catgcaaaga aagtgatgag cgcgctctgg gggctcgggc
      181 acatgatgat gctcacgcgc gtgctgatcg tcgtcgatgc cgacgtcgac gtgcaggata
      241 cccgcgccgt tgcgtggttc gtgctcaaca acctcgcgcc cgaacgcgac gtcgtgatga
      301 tgccgggacc ggtcgacgac ctcgaccacg gttcgtacag catcgcctac ggtacgaaag
      361 ccgggatcga cgccacccgt aaggatgccg ccgaggggta tacccgcgaa tggccgcccg
      421 acacggtcat ggagcaaagc gtgcgcgagc gcgtcgccga acgatggcac gaatacggtc
      481 tggacgccgt cgcccgcgcg ttgcgcgaat ccgacggatg gtcgggtcaa ggaccgcaag
      541 cttacgccaa gctggtggcc ggcgccgcgc cgccgggcga agcgggcgcg cgatgaaagc
      601 cgtcttgttt ctcaaagagt tgcggatcga gcacacgctc ttcgcgctgc cctttgcgta
      661 cgtcggcgcg ctgctggcgg cgcgcggctt cccatcgtgg gccgcactcg gctggataac
      721 gctcgcggtc gtcggcgcgc gaaccgccgc gatggccgcg aaccggtatc tcgatcgcga
      781 catcgacgcg cgcaatccgc gaacggcgcg gcgcgcggtc gccagcggcg cgctcgctcc
      841 ggctacgatg ctgtgggcgg tcgccgccgg cctgatcgtg ctgttggggg cggcctggat
      901 gctcaacccg ctttgcgtcg agctcatgcc ggtggccgcg ctggcgctcc tgatctatcc
      961 gctctgcaag cgcttcacct ggctcgcgca cttcgtactc ggcgcggtcg acgcgctcgc
     1021 cccgctcggc gcctacatcg gcattgcggg aagagccgac tggccggcgg tgctgctgtt
     1081 cttggcggtc accctatggg tcgccggatt cgacatcatc tacgcgctga tggatcttcc
     1141 aatcgaccgc gcgcagggta tccggtcgct gccggcgcgg ttcggcgaaa ccagcgccca
     1201 cgcgctcccg ctggcgctcc acctcgtcat gctcggattg ctcgcgtggg ctggatggac
     1261 ggcgcacgcc ggttggctct actacgccgg cgtcgccgcc gccgcggtgc tcgtcgccta
     1321 cgaacggcgg ctgctcgaca cggcggagaa tccgtttgcg ctcaacgacc gcatcttcgt
     1381 ttccaacatg acgttctcgg tcgtgttttt gggaaccacc gccgcgaact acctcgcgcc
     1441 ggcagctcga ggcggcggat gaggcgccgc cggttcctca cggggctcgc ggcgggagcc
     1501 gcggcgacgg cggcgtcagc ccgcgcgcaa gtgccgctcc cgaatcagca atttcttcag
     1561 cagctcacgg tcggcgtcaa cgtgacgctt tcggggccgc tcgaaaagta cggagccgac
     1621 gtcgttcgcg gagcgcaggc cgccgtcgac gagacgaatc gctttaccac cccgatcaat
     1681 cacgtctggg ggctgcgcgc cttcgacgat cgcaacgatc cggcgctcgc cgctaccaac
     1741 gccagcgtcg ccgcggccga cccgagcgtc gtcggcatcg tcggaaacct caccgcaccg
     1801 atgacgctgg cggccctgtc gcgctacgcc aacgtcaact tcgcggtggt cgtcccgacg
     1861 gtaaccgccg acgcgatcac ggcgcgcgga tttcacaacg tgttccggct tcccgccaac
     1921 gacagcgcgg ccggccggct gttcgccggc gcggtgctgc ccgacaagaa gagcgccccc
     1981 gcactcgccg tcgcattcga cggagactac ggcttcgacg tcgcgcgcgg cttcgtcgca
     2041 caggccaaag cggaccggca tccggccgac gtcctgctct ttccaacgaa cggcgtcgac
     2101 ccggcggcgg cggcgcgcac ggtgctcgac cgcagccccg gttacgtatt cctgtgcgga
     2161 aaaaccgcgg cgctcgggcc ggtcgccgcg gcgctgcgcc tcgccggcta caccggcgaa
     2221 ttcggcgcga gcgacggttt ctacaacggc gagacgctga cgagctacgc caaagttctc
     2281 gacggcgcgt tcgtaagcgc gtcgctgccg ccgctcgaac gcgtccccag catcatggcg
     2341 ctgttgaccg attttcaacg cgaagtcggg gcgattaccg cgttctccgc gttcggttac
     2401 gcggccgcgc aggtgctgat cgcggccgcc cagcgcagcg gcgccacgac gcgcttttcg
     2461 ctgctgccgg cgatgcagcg ccagtccacc ttcatcacgc tggtcgggca gtacagcttc
     2521 agcgttaccg gcgacccgct gatccccaac gtctacctct acaccgtcac caaggacggc
     2581 ttcaagttcg cgcgacccgc gatccggacg gggttcgtgc tgtaagaacg gccctcgacg
     2641 gcgcgttaac ggacgtactc gtcttccggg acgacccgca tacgatgcgg cggagccgcc
     2701 ttgcgcgccg gctgcgcgtc ggtgtcctcg atggcgagca cgaagcgcaa gcgccacgca
     2761 ccgagcgaga ggacgaagtt ggttccgaga atctccatgt ccctatgcct ccacgcgacg
     2821 ccactgccat acggccagcg ccaagtaaat caaagcgagg atcgcgagcg ccggcagctc
     2881 gtgaagggga tctacggcga gcgatccttt gggctgtccg tcgacggtca ccgccggccc
     2941 aaagtgcaag tagaaaaacg gattgatcca ggcgaacggg gttgcgatcg cgtggacgac
     3001 ttgcccgagt gcgctgcctt gcagcggcag ccgcgcaaac aagacgacca gcgcgctcac
     3061 gggccacgac agacctagga cgacgccgaa ggcccgccgc atcgacgcgg tcgccgcgca
     3121 cagcatggcg taccacgccg tcacgccgac cagccccaag acgacgatcg tcgcgtcgac
     3181 cggccgaaat atgaagtgcg gtgcttcgaa aatcgagtgc ccgacgacca gcgcgatgac
     3241 ggccaccgcc caagcggcca gaatgccgag caggtccacg ccaatcgttt gcagcgcgag
     3301 ggcgctgcgg tcgatcggtt tcgtcagcgc gatctccaaa tggccgtcgt tctcccgagc
     3361 gaacggtgcc cccagcaccg tcgccacgat caacgcgatg aaggtagcga tggcggcgta
     3421 gtaggcaaag ctttcgggct ggttcgtctc gatcgtcgtc accgtttcct ggccttgggc
     3481 ggtcgactga acgttcaagc tgcccatctc gatcgtcttg ggagcgtgcc tctttccatg
     3541 agacgacgac tcgtagatcg taatgcgctt cccctgatag ccgctgtcgt cgatgacgat
     3601 gtgcacgtct tccttggcgt tatcgacggt cgtgcgccgg gttccgtcgg gcagcgtcga
     3661 atgggcgacg cgcgagttcg cgtcggtttc catcccattt acgaacgaga gcgcgtcgtc
     3721 tttcccgaac gcgagcagcg aaacgcgcag aacgagggcg atgaggacga gcgcgcctaa
     3781 aacgactacc gtccacttca gcgcggcgcg gacgcgcagc cattccacgt aggtcatgag
     3841 gggctccctt gagcgacgac gtcggccgtc gcatccgacg gcgagacggc gtaaagaaag
     3901 atgtcttcca gattgaggtc gagcacgcgc acgccggagg cgccggcgtt cgtaagccgg
     3961 gcggcgatct cgtcggcatc gcgcagcacg agcagccgga cgatgcgctc ggtgcgatcg
     4021 aggcgcgcga cccgcgggtc gccggcgata ccgtcgagtg cgtagtcgac gccggggaag
     4081 atgccctcga cgatcttgcg gtcggctttc aggtcgtcga tcgcgccgtc gaggatgagc
     4141 cggccgcggt cgagcacgcc gattcgctcc gccgcgcgct ccacctggcc gacctgatgc
     4201 gacgaaaaaa ttaccgtgtt gccgcgagcc gactcgttga tgatgaggct cagcacgtgg
     4261 cgctgattga tcggatcgag cccgctggtc ggttcgtcga gaatcaggat cgcggcgttg
     4321 cgcgcgaagg cgagcgcgac catcactgcg gtgcgcatgc ctttcgagag cgcgcgagcg
     4381 cgtttgcgcg gatcgatgct aaactggcgc agcagctcgg cggcgcgcac cggatcgaaa
     4441 ttcgcgaacg cgcggcgatt catctcgacg tgttcggcga cggtcatcca ctcgtacagc
     4501 acgctgcgtt cgggtacgta cccgacgtcg tggaaggtcg ccggctcgag cggtttgccg
     4561 tcgtaaagga tctggccggc ggacgggcgt gccaggccga gcatgcactt gaaggccgtg
     4621 gtctttccgg cgccgttggg gccgagcaaa ccgaatacgg taccgctggg gatctcgagc
     4681 gagacgtcgt cgacggcggc cagcggtccg tacgtcttgc gcaagtgtga aatttggatc
     4741 gtgctcatcg ttcctccgga aaccagcggt cgagcgccgc gtcgatcaag ccgcgcacct
     4801 cgtcgcgagt gagtcccacc gatttggcct cgcgcaggcc gaggacgagc gcgtcgcgag
     4861 cgacgtcgct ggcggcgctc cgcgcc
//
LOCUS       DASUCJ010000501         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_662538, whole genome shotgun sequence.
ACCESSION   DASUCJ010000501 DASUCJ010000000
VERSION     DASUCJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662538"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2366
                     /locus_tag="VFU90_05970"
     CDS             303..2366
                     /locus_tag="VFU90_05970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5479358.1"
                     /translation="MSALCAVCVAATAPRAQDSLGNNRFALRDIFELQWAADPQISPD
                     GRQVVYARSRYDIMKDRERSTLWVVSADGGEQRPLTSGGERNETSARWSRDGERIAYV
                     SNADGASEIHVRWLASGVDGIVTHVEHGPSALAWSPNGEFIAFAMFVPEPVKPLVTLP
                     AKPDGADWGPPTRYTDALVYRRDGGGDVPSGHVHIFVVSADGSAPRQITSGPFDDGAP
                     VWSPDGKTIYFSANRHPDGNYTPLNTEVYAVPVAGGAVRALTTRDGPDNSPAISPDGR
                     MVAYTGFDDHHQGYQVTHLYVMNADGSSPREVTPNFDRDLFAPSWAADGRGIYALYDD
                     QGDTKVGLVTLDGKVTRIVSHVGGLDIGRPYPGGSYSTSHVGRIAFTETDPDHPADVA
                     VTGARAGSVARLTRLNDGLFGPGGKTLGAVQEIHVKSSFDQRDVEGWVITPPGFDASH
                     KYPLLLEIHGGPYLDYGDRWSAELQQYAAAGYVVLYMNPRGSTSYGETFGNLINHDYP
                     DHDYDDLMAGVDAMIAKGYIDPDNLYVTGGSGGGVLTAWIVGHTHRFRAAVVQKPVIN
                     WYSWVLTTDLSEFGLTYWFTGAPWTQADTYMKYSPITYVGNVTTPTMMITGDVDYRTP
                     SSEAEQFYEALKLRKVPAALVRVPNASHEIAATPSHMMVKIAYVLAWFKKWQRAGAQN
                     AVGQR"
BASE COUNT          429 a          718 c          899 g          405 t
ORIGIN      
        1 atcgatccgt tgtttgttga acaacaacac aacagcacgg acatgcgacc aggctagccg
       61 gcgcggagca gccgagcgat ccgtgctgct atccgtgctg tcatccgttc aatccgtgtc
      121 tccggagcgt ggaagctcgc cacctcgcaa ctgctccggc attggccgcg cgtcgggcgc
      181 cccaacttgg accggtaccc tcgctcggga gagcttgcgc gggggagtcc ggtgcccaac
      241 ttaaaagcgg cgatggtggc gcgttgggcg tccctgaatc gggagatgtg atgcgaacgt
      301 tgatgagtgc actgtgtgcg gtgtgtgtgg cggcgacggc gccgcgggcg caggattcgt
      361 taggcaacaa ccgattcgcg ctgcgcgaca ttttcgagct gcagtgggcg gccgatccgc
      421 agatttcgcc ggatgggcgg caggtggtgt atgcgcgttc gcgctacgac atcatgaagg
      481 atcgcgagcg atcgacgctg tgggtggtga gcgccgatgg cggcgagcag cgtccgttga
      541 cgagcggcgg ggagcggaac gaaacgtcgg cgcgatggtc gcgggatggc gagcgcatcg
      601 cgtacgtgtc gaacgcggac ggcgcgagcg aaatccacgt gcggtggctg gcctcggggg
      661 tggacggtat cgtgacgcac gtggagcacg ggccgtcggc gctggcgtgg tcgcctaacg
      721 gggagttcat cgcgttcgcg atgttcgtgc cggaaccggt caagccgttg gtcacgctgc
      781 cggcgaagcc ggatggcgcg gactgggggc cgccgacgcg gtacaccgac gcgttagtct
      841 accggcgtga tggcggcggg gatgtgccat cggggcacgt gcacattttc gtggtgagcg
      901 cggatgggag tgcgccgcgt cagatcacga gcgggccgtt cgacgacggc gcgccggtgt
      961 ggtcgcccga tgggaagacg atttactttt ctgccaaccg gcatccggac ggcaactaca
     1021 cgccgttgaa tacggaggtc tacgcggtgc cggtggcggg cggggcggtg cgcgcgctga
     1081 cgacgcgtga tggccccgac aactcgccgg cgatttctcc agacgggcgg atggtggcgt
     1141 acacgggatt cgacgaccac catcaggggt accaggtgac gcatctgtac gtgatgaacg
     1201 cggacgggtc gtcgccgcgc gaggtaacgc ctaacttcga tcgcgacctg ttcgcgccgt
     1261 cgtgggcggc ggacgggcgc ggaatctacg cgctgtacga tgaccagggc gacacgaagg
     1321 tcggattggt gacgttggac gggaaggtga cgcggatcgt ctcgcacgtc ggcgggttgg
     1381 acatcggtcg gccgtaccct ggcgggtcgt actcgacgtc gcacgtgggg cggatcgcgt
     1441 tcacggagac cgacccggac catccggccg acgtcgcggt cacgggcgcg cgggccggct
     1501 ccgtggctcg cctcacgagg ctcaacgatg gattgttcgg gccgggcggg aagacgttag
     1561 gcgcggtgca ggagatccac gtcaagtcgt cgttcgacca gcgcgacgtg gaagggtggg
     1621 tgatcacgcc gccgggcttc gacgcctcgc acaaatatcc gctgctgctc gagatccacg
     1681 gcggtccgta tctcgactac ggcgaccgct ggtcggcgga gctgcagcaa tatgcggccg
     1741 cgggctacgt cgtgctctac atgaacccgc ggggcagcac cagctacggc gagacgttcg
     1801 gcaatctcat caatcacgat tacccggacc acgactacga cgatctgatg gcgggcgtgg
     1861 atgcgatgat cgcgaaaggg tacatcgacc ccgacaatct ctacgtcacg ggcggcagcg
     1921 gcgggggggt gctcacggcg tggatcgtcg ggcatacgca ccggttccgc gcggcggtcg
     1981 tgcagaagcc ggtgatcaac tggtacagct gggtgttgac gaccgacctg tccgagttcg
     2041 gcctaacgta ctggttcacg ggcgcgccgt ggacgcaggc cgacacctac atgaaatatt
     2101 cgccgatcac ctacgtgggc aacgtgacga cgccgacgat gatgatcacc ggcgatgtcg
     2161 actaccggac gccgagctcg gaagccgaac agttttacga ggcgctcaag ctgcgcaagg
     2221 tgccggcggc cctggtgcgc gtgcctaacg catcgcacga aatcgcggcg acaccgagcc
     2281 acatgatggt gaagattgcc tacgtgctgg cgtggttcaa gaaatggcaa cgcgccggcg
     2341 cgcagaacgc ggtgggccag cggtagcgga tgtggaacgc ccccgcatac ctgtcggcac
     2401 aggtaccggg ggcgccccca ctcggaggaa gtagaagccc gaaggcttca a
//
LOCUS       DASUCJ010000502         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_677874, whole genome shotgun sequence.
ACCESSION   DASUCJ010000502 DASUCJ010000000
VERSION     DASUCJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677874"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..491
                     /locus_tag="VFU90_05975"
     CDS             69..491
                     /locus_tag="VFU90_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU5479359.1"
                     /translation="MATWRDVRRIALALPETSEGVSFGKASWSVKGKLFAWERPLRRS
                     DLAALGDAAPNGAILGVRTPDLEMKEVLLARNPGVYFTTPHFDGYPAVLIRLAKIRSA
                     ELKSIVLEGWLARAPQRVAGAFLQERGRPAHPKRTPRR"
     gene            564..1907
                     /locus_tag="VFU90_05980"
     CDS             564..1907
                     /locus_tag="VFU90_05980"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5479360.1"
                     /translation="MDTAASQRRATMEEITALAKRRGFVFQSSEIYGGINGFWDYGPL
                     GAVLKRNVKEAWWRDTVELRDDVVAFDSSIVMHPLVWTASGHVEAFNDLLVDCKVCKH
                     RFRFDHLKDRTKCPDCGSKDSFTEPRSFNLMMKTFVGPMEDTSSAAYLRPETAQGIFV
                     NFKNIYQTTRRRPPFGIAQIGKSFRNEITPGNFTYRVREFEQAELEYFVPDDGKDLEV
                     FREWVERRKAWYSNYGIAPERLRFNELSESERPHYARAGVDVEYAFPWGWGELESIAH
                     RGTYDLDAHMKLSGKDLQFFDEASKSHYTPLLIESSAGMDRTALTFLVDAFERESSVD
                     PNGKQTERTVLRFHPQIAPVQAAVFSLARNKPELVERARAIEARLRPRLRTQYDEGNI
                     GQLYRRQDEIGTPLCVTVDYETIADGSVTVRARDSMRQERVSAEALESHLAERLA"
     gene            1904..>2151
                     /locus_tag="VFU90_05985"
     CDS             1904..>2151
                     /locus_tag="VFU90_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5479361.1"
                     /translation="MTPQTSAKSVYFFEEGSASMVDELGGKGAGLAEMTSAGLPVPSG
                     FTITAGTCLRFYEAGRTFPAGLQDEVDAAMRELERRTG"
BASE COUNT          394 a          671 c          723 g          363 t
ORIGIN      
        1 agctgcgggc ggagacgcag ccgaaggcgc accccgccgg tgcactgcgg atccccagcg
       61 tgtaagccgt ggcgacctgg cgggacgtcc ggcggatcgc gttggcgttg ccggaaacga
      121 gcgaaggcgt ctcgttcggt aaagcgtcgt ggagcgtgaa gggcaaactg tttgcgtggg
      181 agcgtccgtt gcgccgctcc gaccttgcgg cactcggtga cgccgcgccg aacggggcga
      241 tcttgggcgt gcggacgccg gacctcgaaa tgaaagaggt gctgctcgcg cgcaacccgg
      301 gcgtatattt tacgacgccg catttcgacg gctacccggc ggtgctgatc cggctggcaa
      361 agatccgaag cgccgagttg aagagcatcg ttctcgaggg gtggctcgcg cgcgcgcccc
      421 agcgcgtcgc cggcgcgttc ttgcaggagc gcggacgccc ggcgcatccc aaacgcacgc
      481 cgcggcgtta acggtcgcgg gagggccgcc gcgacggccc tcttgcgggt atccggggga
      541 ccgccggaaa acctccggcc ggtatggaca ccgcggcttc gcaacggcgc gcgacgatgg
      601 aggagatcac ggcgctggcg aagcgccgcg gcttcgtttt tcaatcgagc gaaatctacg
      661 gcggcatcaa cggcttttgg gactacgggc cgctgggcgc cgtactcaag cgcaacgtta
      721 aagaggcgtg gtggcgcgac accgtcgagc tgcgcgacga cgtcgtcgcg ttcgactcct
      781 cgatcgtgat gcatcccctc gtctggaccg cttcggggca cgtcgaagcc ttcaacgacc
      841 tgctcgtcga ctgtaaggtc tgcaagcacc gcttccggtt cgatcatctg aaagatcgca
      901 cgaagtgtcc cgactgcggc agtaaggatt cgtttaccga gccgcgcagc ttcaacctca
      961 tgatgaagac gttcgtcggc ccgatggaag acacgtcgtc ggcggcctat ctccggcccg
     1021 aaaccgctca ggggatcttc gtcaacttca agaacatcta tcaaacgacg cggcgccggc
     1081 cgcctttcgg catcgcgcag atcggcaaat cgtttcgcaa cgaaattacg cccggtaatt
     1141 ttacgtatcg cgttcgcgaa ttcgaacaag ccgagttaga gtacttcgta cccgacgacg
     1201 gcaaggactt ggaggtcttt cgcgagtggg tcgagcggcg caaggcgtgg tattcgaatt
     1261 acggcatcgc ccccgagcgg ctgcgcttta acgagttatc cgaatccgaa cgcccgcact
     1321 acgcgcgcgc cggcgtggac gtcgagtacg cctttccgtg gggctggggc gagctcgaat
     1381 cgatcgcgca ccgcggcacg tacgacctgg acgcgcacat gaagctttcg gggaaggact
     1441 tgcagttctt cgacgaggcc agcaaatcgc attacacgcc gctgctgatc gagagctccg
     1501 ccggtatgga ccgcacggca ctcacgttct tggtcgatgc gttcgagcgc gagagcagcg
     1561 tcgatcccaa cggcaagcag accgaacgga cggtcttgcg gttccatccg cagatcgcgc
     1621 cggttcaggc ggcggtcttt tcgctggcgc gcaacaagcc cgagctggtc gaacgggccc
     1681 gcgcaatcga agcgcgcctg cgtccgcgtt tgcgcacgca gtacgacgaa gggaatatcg
     1741 ggcagctgta ccgccggcag gacgaaatcg ggaccccgct gtgcgtgacc gtcgactacg
     1801 aaacgatcgc cgacggaagc gtcaccgtgc gggcgcgcga ctcgatgcgg caggagcgcg
     1861 tatctgccga agcgctcgag agccacctcg cagaacggct ggcatgacgc cgcaaacgtc
     1921 tgccaagtcc gtctatttct tcgaggaagg gagcgcgtcg atggtcgacg agctcggcgg
     1981 aaaaggggcc ggtttggcgg agatgacgtc ggccgggttg ccggtgccgt cgggatttac
     2041 gatcaccgcc ggcacgtgtc tgcgctttta cgaagccggc cgcacgttcc cggccggcct
     2101 ccaagacgaa gtcgacgccg cgatgcgcga gctcgagcgg cgtaccggta a
//
LOCUS       DASUCJ010000503         1381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_97358, whole genome shotgun sequence.
ACCESSION   DASUCJ010000503 DASUCJ010000000
VERSION     DASUCJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97358"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFU90_05990"
     CDS             complement(<1..1260)
                     /locus_tag="VFU90_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479362.1"
                     /translation="MVIARGLLVLAGAIALNAAAATLLPLDVGAQHVPGFLNPTVAGV
                     IAADFEGIGHDDIAVAGMQRPGSPYGMTSGEAHSGFVAILRFNTQSQTYAIVQTLVTD
                     DADLAGIALDPTSSAGPGLVVVSQAGTLTRYSGWPLQLISHVSLKQYVSGMQVGDVDG
                     DGRPDVVVTHPVSGLAAYDLVTGALERTYAGRAGNSLALAQLDADPALEIVLSGTPGY
                     VVDGATNATDWEYAGGIGGVLAAGTVLAGNGQGFVAAESRYLSAFRSTPYSPIWSLDG
                     GYTAVDFAAIDGSPRLLLGSSDGVLEVLDPVTQAIQANLVADPVGHLAAGHLEDATVL
                     QVVSSSGLSTTAGDSLQIHGFDGSLRFALDDEDGPFVVAAPGDVDGDGRADGLWASYY
                     SASEYEGGYIHIYDPITGAERSRAMLTD"
BASE COUNT          232 a          462 c          467 g          220 t
ORIGIN      
        1 gtcggtcaac atcgcgcgcg agcgctctgc gccggtaata ggatcgtaga tgtgtatgta
       61 tccgccctcg tactcgctgg cgctgtagta cgaggcccac aggccgtcgg cgcgtccgtc
      121 gccgtcgacg tcgcccggtg cggcgaccac gaacgggccg tcctcgtcgt caagcgcgaa
      181 gcgcaggcta ccgtcgaagc cgtggatctg gagcgaatcg ccggcggtgg tcgagagccc
      241 gctcgacgag accacttgca gcaccgtagc gtcttccaga tgcccagcgg ctagatgtcc
      301 caccgggtcc gccacgaggt tcgcctgtat ggcttgggtc accgggtcga gtacttcgag
      361 tacgccgtcg ctgctgccga gcaacaggcg cgggctgccg tcgatcgcgg cgaaatccac
      421 cgccgtgtag ccgccatcga gcgaccagat aggcgaatac ggcgtgctgc ggaacgccga
      481 gagatagcgg ctttccgcgg cgacgaagcc ctggccgttg cccgccagca cggtgcccgc
      541 ggcgagcacg ccgccgatgc cgccggcata ttcccaatcg gtcgcgttgg tggcgccgtc
      601 gaccacgtag cccggtgtac ccgagagcac gatttcgagc gccggatcgg cgtcaagctg
      661 cgctagcgcg agcgagtttc cggcgcgtcc ggcatacgtg cgttctagcg cgccggtgac
      721 gaggtcgtaa gcggccaggc cgctgaccgg atgcgtgacg acgacgtcgg gacggccgtc
      781 tccgtccaca tcgccgacct gcatgccgct cacgtattgt ttgagcgaca cgtggctgat
      841 gagctgcagc ggccagccgc tgtagcgcgt cagcgtgccg gcttgcgaca cgaccaccag
      901 cccggggcca gcgctggagg ttgggtcgag cgcgattccg gcgaggtccg cgtcgtcggt
      961 taccagcgtc tgcacgatcg catacgtctg gctctgcgta ttgaaacgca ggatcgcgac
     1021 gaagccggaa tgcgcttcgc cggacgtcat gccgtagggt gagcctggac gctgcatgcc
     1081 cgcgacggcg atgtcgtcgt ggccgatgcc ttcgaaatcg gccgcgatca caccggcgac
     1141 cgtcgggttg agaaaaccgg gcacatgctg cgcgccgacg tcgagcggca acagtgtcgc
     1201 cgccgcggca ttcagcgcta tggcaccagc cagaaccaac aaaccgcgcg cgatcaccat
     1261 gaatcgaatc cgtccgagaa cagtgcatcg ttcgagtcga gcgtcagcgc ccacacgccg
     1321 acgccgtcgc cgacgtacgc ggtggtgacg ccgccttgcg cacgcgtgcg caggatgcca
     1381 t
//
LOCUS       DASUCJ010000504         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_937805, whole genome shotgun sequence.
ACCESSION   DASUCJ010000504 DASUCJ010000000
VERSION     DASUCJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937805"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..>1035
                     /locus_tag="VFU90_05995"
     CDS             399..>1035
                     /locus_tag="VFU90_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479363.1"
                     /translation="MRGIISAAGYVPYRRLQRSEIAKLFGAGGGRGTRSVASYDEDTT
                     TMGVEAARLALHRAGSTAPSVLWFATASPAYLDKNNASTVHAALRLDSDIAAFDFGGA
                     LRSSVGALGAALKSRDAVLVVASDLRTGLPTSADEAQTGDGAAALIVGDDTDGPVLAE
                     FLGGAHATEEFLERWRTPGSTRSRAWEEKFGEIKYAPLVEQAWNGALKAAEL"
BASE COUNT          167 a          352 c          366 g          150 t
ORIGIN      
        1 gacaacaacg gcgacagtcc agttgcgcag tcgatcgaag cgccgcatct cctgattatc
       61 ccgtttggag caaacgtttg ggaacctgcc gcagactcgc agcaaccgca cagcgcacgc
      121 tcaaagaact catcgggcgg cgcggtctac tcgtcgtgcc acgacgcggc gcgtaactgc
      181 ttgggcgcgg cgctggtggg cacgacgcgc gcctccacgg atgggggcgt gcccgtcggg
      241 gcgcgaacgc cgtcacctca cctctgccgc ccggcatcga aggagggacg catgccatcg
      301 acgtgtgctt ctacggcaat cggaagcgcg atcgccgccg ccggtgcgct gaatcggcct
      361 cggaggccgg tcccggccgg tcggtagact gcccgttcgt gcgaggcatc atcagcgccg
      421 ccggatacgt gccgtatcgc cggctgcaac ggtcagagat cgcgaagctt ttcggcgcag
      481 gtggtggccg gggcacccgt tcggtcgcgt cctacgacga agacacgaca accatggggg
      541 tcgaggctgc ccggctcgcg ttgcaccgcg ccggcagcac ggcgccgagc gtgctgtggt
      601 tcgcgaccgc gagccccgcg tatctcgaca agaacaacgc gtcgaccgtg cacgcggccc
      661 tgcggctcga cagtgacatc gccgcgttcg acttcggcgg ggcgttgcgg tcgagcgtcg
      721 gcgcgttggg tgccgcactc aagagccgcg acgccgtgct cgtcgtcgcg tccgatctcc
      781 gcaccggctt gcccacgagc gccgacgagg cgcagacggg cgacggcgcg gccgcgctga
      841 tcgtcggcga cgacaccgac ggcccggtgc tcgccgagtt cctcggtggc gcgcacgcga
      901 ccgaagagtt cctcgaacgg tggcggacgc ccggttcgac gcgatcgcgc gcatgggaag
      961 agaagttcgg cgagatcaag tacgcgccgc tggtcgaaca agcctggaac ggcgcgctga
     1021 aagcggccga gttgc
//
LOCUS       DASUCJ010000505         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_97362, whole genome shotgun sequence.
ACCESSION   DASUCJ010000505 DASUCJ010000000
VERSION     DASUCJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97362"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFU90_06000"
     CDS             complement(<1..334)
                     /locus_tag="VFU90_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479364.1"
                     /translation="MRDDDTIAAVATPPGKGAIAIVRVSGPGVRAVAKRVVRARAPLP
                     ARQATPATLLDEAGRPIDRGLALFFPAPASYTGEDVLELHVHGSPAVAREVMRALIAG
                     GARHAGPGE"
     gene            complement(348..806)
                     /locus_tag="VFU90_06005"
     CDS             complement(348..806)
                     /locus_tag="VFU90_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479365.1"
                     /translation="MAYHHEQIGRFYVAMLAVAALMVVAAAVVVGAVGERAPAGFPGF
                     VAALLGIIIVTFCRLNIDVGATELRWSMTFGWPYGRIPIADIAGAEIVPVTFWMGIGI
                     HLTLRGWLWNVALGKGVRIRKRDGIAIVLGTDDPEGLLTAIANVRAADTP"
     gene            complement(831..1490)
                     /locus_tag="VFU90_06010"
     CDS             complement(831..1490)
                     /locus_tag="VFU90_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU5479366.1"
                     /translation="MLYEDDFEFEEQPANIRDRAIPGTSGGRGPGGDRRRGGGGGGRG
                     RGNGGRRYGPPLHHTGPSTLEHFELPAEAAPARELLVEILGLMGVGPVDIHYIPRSEG
                     EYLEVTGPDLGMLIGRHGNTLEALNLVFNNILNAGVRTNRRYYTIDAEGYRARRAEQL
                     KSLAFATRERCLRDGAPQKLEPMLPSERKIVHLALADSPAVRTESEGEEPERRVVVFP
                     V"
     gene            complement(1500..>2080)
                     /locus_tag="VFU90_06015"
     CDS             complement(1500..>2080)
                     /locus_tag="VFU90_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU5479367.1"
                     /translation="NPLAGCWPMLVQWPFILSVYYVVMNNKTHFENQNWLWIGSHLST
                     LYPAFLATSLAHSDVVLLVLYAVSMYLSVRFGSMPATDPQQAQTQRIMSFFSPLMLGF
                     FGWRYHWPSAMVLYWFSYNAFTMAQTFYMLRRYHEPLSFADSAHVITDDVDGGKPSKA
                     LPKNAAGSNGASQKTTPRPASSRSKKKNKKGA"
BASE COUNT          351 a          690 c          692 g          347 t
ORIGIN      
        1 actcgcccgg cccggcgtga cgcgccccgc cggcgatcag tgcgcgcatc acctcgcgcg
       61 cgacggcggg cgaaccgtgg acgtgcagct cgaggacgtc ttccccggtg tagctggcgg
      121 gggccggaaa gaacagcgcc agcccgcggt cgatcggccg gccggcttcg tcgagcagcg
      181 tggcgggcgt cgcctgccgc gccggcagcg gcgcgcgcgc tcgtacgacg cgtttcgcaa
      241 ccgcgcgaac gcccgggccg ctgacgcgca cgatcgcaat cgctcccttt cccggaggcg
      301 ttgcgaccgc tgcgatcgtg tcgtcgtcgc gcaccggtgc ggacgcctta cggcgtgtcg
      361 gcggcacgca cgtttgcaat cgccgtcagc agcccttcgg gatcgtcggt gccgagcacg
      421 atggcgatgc cgtcgcgttt gcgaatccgc acccccttgc cgagcgccac gttccacaac
      481 cagccgcgca gcgtcagatg aatcccgatg cccatccaaa acgtcaccgg cacgatctcc
      541 gcgccggcga tgtcggcgat cggaatccgg ccgtacggcc acccgaacgt catcgaccag
      601 cgcagttcgg tcgcgccgac gtcgatgttc aagcggcaaa aggtgacgat gatgataccg
      661 agcagagccg cgacgaagcc ggggaaaccc gccggcgccc gctcgccgac ggcgccgacg
      721 accaccgccg ccgctaccac catgagggct gcgacggcga gcatcgcgac gtaaaagcgc
      781 ccgatttgtt cgtgatggta ggccacgggg cagcctcccg gttaaggccg ctacaccgga
      841 aagacgacga cccgccgttc gggctcttcg ccttccgact cggtgcggac cgccggcgaa
      901 tcggccagcg cgaggtgaac gatcttgcgc tcagacggaa gcatcggctc gagtttttgc
      961 ggcgcgccgt cgcgcaggca acgctcccgc gtggcaaacg ccaaactctt cagctgctcc
     1021 gcgcgccgcg cgcgatatcc ttcggcatcg atcgtgtagt agcgccggtt ggtgcgcacg
     1081 cccgcgttga gaatattgtt gaacacgagg ttgagcgctt cgagcgtgtt gccgtggcgg
     1141 ccgatgagca tcccgaggtc cgggccggtc acctcgagat actcgccttc ggagcgcggg
     1201 atgtagtgga tgtcgaccgg cccgacgccc atcaggccga gaatttccac gagcaattcg
     1261 cgggcgggcg ccgcttccgc gggcagctcg aaatgctcga gcgtgctcgg cccggtatgg
     1321 tgtaagggcg gtccgtagcg gcgtcctccg ttgccgcggc cgcgtccgcc gccgccgccg
     1381 ccgcgccgcc ggtcgccgcc gggtccgcgc cctccggaag ttccgggaat cgcgcgatcg
     1441 cggatgttcg cgggctgctc ttcaaactcg aaatcgtctt cgtacagcat cgtaagcgtc
     1501 tatgcgcctt tcttgttttt cttcttggac cggctcgaag cgggccgcgg agtggttttt
     1561 tgggacgcgc cgttggagcc ggcggcgttc ttcggcagtg ccttggaagg tttgccgcca
     1621 tcgacgtcgt cggtgatgac gtgggcgctg tcggcgaacg aaagcggctc gtgatagcgg
     1681 cgcagcatgt aaaacgtttg cgccatcgta aacgcgttgt acgagaacca gtacagtacc
     1741 atcgcggacg gccagtgata ccgccatccg aagaagccga gcatcagcgg tgagaagaac
     1801 gacatgatcc gctgcgtctg agcctgttgc ggatccgtcg ccggcatgct gccgaagcgg
     1861 acgctcagat acatcgaaac cgcgtagagg accagcagca ccacgtcgct gtgggccaag
     1921 ctggttgcca agaacgcggg atagagggtc gacaagtggc tgccgatcca cagccagttc
     1981 tggttctcga agtgcgtctt gttgttcatc acgacgtaat aaacgctcag gatgaacggc
     2041 cattgcacca gcatcggcca gcagccggcc agcggattga
//
LOCUS       DASUCJ010000506         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_173441, whole genome shotgun sequence.
ACCESSION   DASUCJ010000506 DASUCJ010000000
VERSION     DASUCJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173441"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1341)
                     /locus_tag="VFU90_06020"
     CDS             complement(<1..>1341)
                     /locus_tag="VFU90_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479368.1"
                     /translation="GGATWTRQNRLDERGNYYGQIRVDPTNPNNVWVPLTALHVSIDG
                     GKTFTEDSVNVRVHPDNHALWIDPHQPDHMMLGNDGGVWFTYDGARHWEYINNLPIGQ
                     LYDIAIDHRDPYWIYGGLQDLGTFTFPSGTHSRGSLTDVGVTFLEYGDGFQVAVDPTD
                     PRFVYTNSQNGRGYVVDLVTREERRITPLSADSAQPYRFNWNTAILLSPNDPHTYYYG
                     ADKLLATSDRGTTWKVLSPDLTRNEKDWKKLSLGEGIPLRDSTTPSRDDGTGLYGNIT
                     TISESSKAAGTMYVGTDDGNVQMTTDGGAPWTNLTPRFHLRAAHWVSAVRASKFDART
                     AYVTFDGHYDDDLAPYVFKTTDGGATWTSIAGDLPPGTPVKTLTEDPRNPSVLFAGTE
                     FGLYWTFDGGRHWQFPGGTLPRVMVDRILVDDSTNDLILGTYGRSVIILDDIRAL"
BASE COUNT          224 a          405 c          447 g          265 t
ORIGIN      
        1 gagcgcgcga atgtcgtcga ggatgatgac gctgcgtccg tacgtgccta aaatgagatc
       61 gttggtgctg tcgtcgacga gaatgcggtc gaccatcacg cgcggcagcg tcccaccagg
      121 aaattgccag tggcggccgc catcgaacgt ccaatagagt ccgaactcgg tgcccgcaaa
      181 gagcacgctg ggattgcgcg ggtcttcggt gagcgtcttg accggagtgc ccggcgggag
      241 atcaccggcg atcgatgtcc acgttgcgcc gccgtcggtg gtcttgaaga cgtatggcgc
      301 gagatcatcg tcgtagtgtc cgtcgaacgt gacgtaggcc gtgcgcgcgt cgaacttcga
      361 cgcgcgaacc gcgctcaccc aatgagcggc gcggaggtga aaccgcggcg tgaggttcgt
      421 ccagggcgca ccgccgtcgg tggtcatctg gacgttccca tcgtccgtcc cgacgtacat
      481 cgttccggcg gccttcgacg attcgctgat cgtcgtgatg ttgccgtaga ggccggtgcc
      541 atcgtcgcgc gacggcgtgg tgctgtcgcg cagcggaatg ccctcgccca ggctcagctt
      601 cttccagtct ttctcgtttc gcgtgagatc cgggctcaac accttccacg tggtgccgcg
      661 atcgctggtc gcgagcagct tgtcggcgcc atagtaatac gtgtgcggat cgttaggcga
      721 gaggaggatg gccgtgttcc agttgaaccg gtacggttgg gccgaatcgg ccgacagcgg
      781 tgtgatgcgc cgctcttcgc gcgtcacgag atccacgaca tagccccggc cgttctgcga
      841 gttcgtgtac acgaagcgcg ggtccgttgg atcgaccgcc acctggaacc cgtcgccgta
      901 ctcgagaaac gtaacgccga cgtcggtgag cgagccgcgc gagtgcgttc cactcgggaa
      961 ggtgaacgtg ccgaggtcct gcaatccgcc gtagatccag tacggatcgc gatggtcgat
     1021 cgcaatgtcg tacaactggc cgatcggcag attgttgatg tattcccaat ggcgcgcgcc
     1081 gtcgtaggtg aaccacaccc cgccatcatt gccgagcatc atgtggtccg gctgatgcgg
     1141 atcgatccag agcgcgtggt tgtcgggatg cacgcgtacg ttcacggagt cctcggtgaa
     1201 cgtcttgccg ccgtcgatcg acacgtgcag cgcggtcagc ggcacccaca cgttgttcgg
     1261 gttggtcgga tcgacgcgga tctggccgta atagtttccg cgctcatcga ggcgattctg
     1321 gcgcgtccac gtggcgccgc c
//
LOCUS       DASUCJ010000507         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_219433, whole genome shotgun sequence.
ACCESSION   DASUCJ010000507 DASUCJ010000000
VERSION     DASUCJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219433"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1203
                     /locus_tag="VFU90_06025"
     CDS             <1..>1203
                     /locus_tag="VFU90_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479369.1"
                     /translation="DPTNGNRFAVAHDGGVAITMNRGRNFLRVQLPIAQMYHVTVDNR
                     IPYDVYGNKQDGTSYMGPSNSKLGADGGIWRGEWRSVGGGESGFAIPDTVDSNLVWSS
                     ASGSGAIGGIVVRYNVPTHIIDNVEVWPENVAGAPADMAKYRFVWNFPLMISPHDHNT
                     VYTGSQFVHRTTNGGQSWDVISPDLTRNDKSKQGISGGLTPDNIGVEYADVIYWIAES
                     PLAKGELWVGTNDGLVQLTRDGGKTWSNVTNLPSGYPTLGTVASIEPSRFTPGTAYMA
                     IDGHQVNNFDPWVYRTTDWGKTWTLITHGIAKSPISYAHTIYEDPARKGLLYLGTEGG
                     MYVSFDAGDNWMPLQSGLPHAPVYGVTVQPHFQDLVIGTYGRGFWILDDLTPLHEWTP
                     ELASKSLAL"
BASE COUNT          238 a          386 c          391 g          188 t
ORIGIN      
        1 cgacccgact aacggaaacc ggttcgcggt cgcgcacgac ggcggcgtcg ccatcacgat
       61 gaatcgcggc cggaacttcc tgcgcgtgca gctgcccatc gcgcagatgt accacgtgac
      121 ggtcgacaat cgcattccgt acgatgtgta cggcaacaag caggacggca cctcgtacat
      181 gggaccgagc aactcgaagc tcggcgctga cggcgggatc tggcgcggcg aatggcgcag
      241 tgtcggcggc ggcgagagcg gcttcgcgat tcccgacacg gtggactcga atctcgtatg
      301 gtcgagcgcg tcgggatcgg gcgcgatcgg cggcatcgtc gtccgctaca acgtcccgac
      361 gcacatcatc gacaacgtgg aagtgtggcc ggagaacgtc gccggtgcgc ccgccgacat
      421 ggcgaagtac cgcttcgtgt ggaacttccc gctcatgatc tcgccgcacg atcacaacac
      481 cgtgtacacg ggctcgcagt tcgtgcaccg gaccacgaac ggcggccaga gctgggacgt
      541 gatcagcccg gacctcacgc gcaacgacaa atcgaagcag ggcatctccg gcggcctaac
      601 gccagacaac atcggcgtcg agtacgccga cgtcatctac tggatcgccg agtcgccgct
      661 agcgaagggc gagctgtggg tggggacgaa cgacggcctg gtgcagctga cgcgcgacgg
      721 cggtaagacg tggtccaacg tcacgaattt gccgagcggg tatcccacgt taggcacggt
      781 ggcgagcatc gagccgtcgc gcttcacgcc gggcacggcg tacatggcga tcgacggcca
      841 ccaggtgaac aacttcgatc cgtgggtcta ccgcaccacc gactggggca agacctggac
      901 gttgatcacg cacggcatcg ccaagtcgcc gatttcatac gcgcacacca tctacgaaga
      961 tccggcgcgc aaaggcttgc tctacctcgg caccgaaggt ggcatgtacg tgagcttcga
     1021 cgccggcgac aattggatgc cgctgcagag cggtctgccg cacgcaccgg tctacggcgt
     1081 cacggtacag ccgcactttc aggatctcgt gatcggaacg tacgggcgcg gcttctggat
     1141 attggacgac ctcacgccgc tgcacgagtg gaccccggag ctcgcgtcga aatcgctggc
     1201 gct
//
LOCUS       DASUCJ010000508         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_387433, whole genome shotgun sequence.
ACCESSION   DASUCJ010000508 DASUCJ010000000
VERSION     DASUCJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387433"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1404)
                     /locus_tag="VFU90_06030"
     CDS             complement(<1..>1404)
                     /locus_tag="VFU90_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479370.1"
                     /translation="FLHAWRNAAIAAISIPASILASFAAMWTAGFTIDVLSLMGLSLT
                     IGILVDDSIVILEAIARRVRAGLAGDEAALAGRRELGPAAIAITLVDVAVFAPIAFTG
                     GIVGAFMREFGLVVVFATAFSLLVSLTLTPLLAARWALRERPAVPVERLPWMLRTRFA
                     GGCAFVIAGLQAAFARSEARVVDVYARVWLPAAFGYSRRFFAGAAVLTVASLVPLATG
                     AIGTEFSPPVDRGSATVDVRFPAGTSLERADAVLNRFSQRLLDDGTIAHVVAIAGRGF
                     NGSYDVAAPNLAQITAVLIDPAASGDAAAGEIKSLQPLAPDAHLAASGRGMGGTAPIR
                     YTIGGARDPADAAARRIAAQLDADPSADDVRLSDAGLEQRLEIDVDSRRAVFLGVSAD
                     DAAQTARIATGGAIAARLRLPGGLTDVLVRGDAAERGDLDAVLAANVRDRGGRLIPLG
                     DVVDVARGTQPAIVEREN"
BASE COUNT          261 a          487 c          474 g          182 t
ORIGIN      
        1 gttttcgcgc tcgacgattg cgggctgcgt gccgcgcgcc acgtcgacga cgtcgccgag
       61 cggaatcagg cggccgccgc ggtcgcgaac gttggccgcc agtacggcgt cgagatcgcc
      121 gcgctcggcc gcgtcgccgc gaaccagcac atcggtaagg ccgccgggca gccgcagccg
      181 tgccgcgatc gcgccgccgg tcgcgatgcg cgcggtttgc gcggcgtcgt cggcggaaac
      241 gccgagaaat acggctcgcc gcgaatcgac gtcgatttcc agccgctgtt ccaaccccgc
      301 gtcgctcaaa cgcacgtcgt ccgccgaagg atcggcatcc agctgggcgg cgattcgtcg
      361 agccgcggcg tcggccggat cgcgcgcccc gccgatcgtg taccgaatcg gcgcggtccc
      421 gcccatcccg cgccccgatg cggcgaggtg cgcgtcgggc gccagcggct gcagcgattt
      481 gatttcgccg gcggctgcgt cgccggatgc cgccggatcg atcaaaacgg cggtgatctg
      541 cgcgagattc ggcgcggcga cgtcgtacga accgttgaaa ccgcgaccgg cgatagcgac
      601 gacgtgcgcg atcgtgccgt cgtcgagcaa gcgttgcgaa aagcgattca gcacggcgtc
      661 ggcgcgttcg agggacgtgc cggccgggaa acgtacgtcg acggtggcgg acccgcgatc
      721 gaccggcggc gaaaattccg taccgatcgc gccggtggcc agcggtacca gcgaggctac
      781 ggtcaggacg gcagcgccgg cgaaaaaacg gcgggaatat ccgaacgccg ccggcagcca
      841 tacgcgcgcg tagacgtcga cgactcgcgc ctcagaccgt gcaaacgccg cttgtaaccc
      901 ggcgataacg aacgcgcacc ctccggcaaa ccgcgtacgc agcatccagg gaagcctttc
      961 gacgggaacc gcgggccgct cgcgcaacgc ccagcgtgcc gccagcaggg gcgtaagcgt
     1021 cagcgacacg agcagcgaaa acgcggttgc gaatacgacg accaggccga actcgcgcat
     1081 gaatgcgccg acgattccac cggtaaaggc gatcggtgca aataccgcca cgtccaccaa
     1141 ggtgatcgcg atcgccgccg gtccgagttc gcgccggccg gctaacgccg cttcgtcgcc
     1201 cgcgagcccg gcgcgcaccc gccgtgcgat cgcctccaaa atcacgatcg agtcgtccac
     1261 cagaatgccg atcgtaagcg acagacccat cagcgagagc acgtcgatcg taaagcctgc
     1321 ggtccacatt gctgcgaacg atgcgaggat cgacgccgga attgaaatcg ccgcgatcgc
     1381 cgcgttgcgc caagcgtgaa gaaa
//
LOCUS       DASUCJ010000509         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_647481, whole genome shotgun sequence.
ACCESSION   DASUCJ010000509 DASUCJ010000000
VERSION     DASUCJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647481"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1362)
                     /locus_tag="VFU90_06035"
     CDS             complement(232..1362)
                     /locus_tag="VFU90_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEU5479371.1"
                     /translation="MIDPSHLPAVVTGLAFALAFVFGAVAQRVNFCTMGAVSDVVNMG
                     DWGRMRMWVLAIAVATAGTGIMQSMGLIDTAKTIYTGERIPWLSHVVGGFLFGFGMTL
                     GSGCGSKTLIRTGAGNLKSLVVLVFLAISAYMTLKGAFAPWRVNGLDTFRVDVHAATS
                     DLPTLAMAMGLPRAVHLWMPLIVAAALAWWIFRDATFRTSRGYIIGGIIIGAVILGGW
                     YVSGHLGFLAEDPQTLEAKYVATDSGRMESFSFVAPSAYLLELLLLWTDQSRLLTFGI
                     AGVLGMLVGSGAMALATRSFRWESFASAEDVGNHIAGGVLMGFGGVTALGCTIGQGIS
                     GVSTLAVGSILALASIIAGAVAAMKYQVWRVERTSAGVRAYA"
     gene            complement(1411..>1741)
                     /locus_tag="VFU90_06040"
     CDS             complement(1411..>1741)
                     /locus_tag="VFU90_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479372.1"
                     /translation="DAGHECVVDGFVTIEARRNLTAKYPNAIEGFERLIATCTTIATP
                     AMAATSLPGLDWLPAKDQPVLAAAIRGRCQVLVTGDRKHFGPGYGRTFGGVAIHSPRS
                     LYLLISA"
BASE COUNT          323 a          591 c          581 g          246 t
ORIGIN      
        1 tcgcgccgac ggaatgcgct cgacgcgcgc atcgtcgcag atcgggcagg tcacgaggcc
       61 gtgctcctgc tgctgcgcaa agtcctcgcc cgaggcgaac cagccctcga agcggtgacc
      121 atgcgcgcag gcgagctcga agatgatcat gtggcagcaa cgaccgtcgg gaggcggcgg
      181 ttgaccgctc ccgcgaccct gatgctacct cagccggggc tgccgccccg gtcacgcgta
      241 cgcgcgcacc cccgcgctcg tgcgctcgac gcgccacacc tggtacttca tcgcggcgac
      301 ggcaccggcg atgatcgacg cgagtgccag gatcgagccg acggcgaggg tcgacacgcc
      361 gctgatgccc tgaccgatcg tgcagccgag cgcggtgacg ccgccgaagc ccatcagcac
      421 gccgcccgcg atgtggttgc caacgtcctc ggcggacgcg aagctctccc agcggaacga
      481 ccgcgtcgcg agcgccatcg ctcccgagcc gacgagcatg ccgaggacgc cggcgatgcc
      541 gaaggtgagc aggcgcgact ggtcggtcca cagcagcagc agctcgagca ggtacgccga
      601 cggtgcgacg aagctgaacg actccatccg tcccgagtcc gtggcgacgt acttcgcttc
      661 cagcgtctgc ggatcctcgg cgagaaagcc caggtgaccc gacacgtacc agccgccgag
      721 gatcaccgcg ccgatgatga tcccgccgat gatgtaccca cggctggtgc ggaacgtcgc
      781 gtcacggaag atccaccacg cgagtgccgc cgcgacgatg agcggcatcc acaggtgcac
      841 ggcacgcggt aggcccatcg ccatggcgag cgtcggaaga tccgacgtcg cggcgtgcac
      901 gtcgacgcgg aaggtgtcga gaccgttgac gcgccacggc gcgaacgcgc ctttcagcgt
      961 catgtaggcg gagatcgcga ggaacaccag cacgaccagc gatttcagat tgccggcgcc
     1021 ggtgcggatc aacgtcttgc tgccacagcc cgagcccagc gtcatgccga agccgaacag
     1081 gaagccgccg acgacgtgcg acagccacgg aatgcgctcg ccggtgtaga ttgtcttcgc
     1141 cgtatcgatg agccccatcg actgcatgat gccggtgccc gcggtcgcga ccgcgattgc
     1201 cagcacccac atgcgcatgc gtccccaatc gcccatgttg acgacgtcgg agacagcgcc
     1261 catcgtgcag aaattgacgc gctgggcgac agcgccgaac acgaacgcga gcgcgaacgc
     1321 gagcccggtg acgacggcgg ggaggtggga aggatcgatc acaggggcaa tcctaccgcc
     1381 ggagcgccat ttggtgtacg cgccccgcgt ttatgcggag ataaggagat atagggaacg
     1441 tggagaatga attgcgacac ccccgaacgt ccgtccgtag cccgggccga agtgcttcct
     1501 gtcgccggtc accaacacct ggcagcggcc gcggatcgcg gcagcgagca ccggctggtc
     1561 ctttgccggc agccagtcga ggcccgggag gctcgtggcg gccattgcgg gcgttgctat
     1621 cgtcgtgcat gtcgcaatca gccgctcgaa gccttcgatc gcgttgggat atttcgcggt
     1681 gagattgcga cgcgcttcga tcgtcacgaa accgtcgacg acgcattcgt gccccgcatc
     1741 g
//
LOCUS       DASUCJ010000510         1393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_372499, whole genome shotgun sequence.
ACCESSION   DASUCJ010000510 DASUCJ010000000
VERSION     DASUCJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372499"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFU90_06045"
     CDS             complement(<1..1191)
                     /locus_tag="VFU90_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479373.1"
                     /translation="MIRLLEYLAQALEALMRNKVRTILTMLGMLIGVAAVLSVYGLSE
                     GAAAAINSNVNSSDNPSLTAAPDPQQADPAIAQLSYRDYRVVEDALGGLATRVTPYYA
                     PSFGQHGIFNVRRQTKHLVAFGWSWYGGDSKFQVLAGRALTAADEHSHAHVAVVNPDL
                     AFEFFGSHDGAIGQQLDVRGTRFNIVGVANDKQGTAAGYFGGNYYFVLPYTTFHDFSP
                     GAVDGLYIWLDSPSDEDTAKQVLLDTLHRVHGKRAAYRVQSNREVLETTKRVVDVVAA
                     GLTAIGAISLLVAGIGIMNIMLVSVTERTREIGIRKSIGASRRDIVVQFLSEAALISV
                     AGGLLGFLLSLLALNVASSALEAKMGTLPIPYGNLFIYGLSFSIVIGITFGVYPALRA
                     SRLDP"
     gene            complement(1205..>1393)
                     /locus_tag="VFU90_06050"
     CDS             complement(1205..>1393)
                     /locus_tag="VFU90_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479374.1"
                     /translation="YVIAGGKARKRAIRTGASNDTQTVILSGVREGDRGIADKNPLLH
                     DGAAVVAQSPSATATPGA"
BASE COUNT          258 a          418 c          455 g          262 t
ORIGIN      
        1 cggatcgagc cggcttgcgc gcagggcggg atagacgccg aacgtgatgc cgatgacgat
       61 cgaaaaactc aagccgtaaa tgaagagatt tccgtacggt atcggcagcg tgcccatctt
      121 ggcctcgagc gcactcgagg ctacgttaag cgcgagcagg ctgagcaaga atcccagcaa
      181 tccgccggcg acggagatca gggccgcctc ggaaagaaac tgcacgacga tgtcgcgtcg
      241 cgatgcgccg atggatttgc ggatcccgat ctcgcgcgtg cgctcggtta ccgaaacgag
      301 catgatgttc atgatgccga tgccggcgac caggagcgag atggcaccta tcgccgttaa
      361 ccccgcggcc acgacgtcga cgacgcgttt ggtcgtttcc agaacttcgc gattgctttg
      421 gacgcggtac gcggcgcgct taccgtggac tcggtgaagt gtgtcgagca atacctgctt
      481 ggccgtatct tcgtcgctcg gcgagtccag ccagatgtac agaccgtcga cggcgcccgg
      541 cgagaagtcg tggaacgtcg tgtacggcag cacgaagtaa tagtttccgc cgaaataccc
      601 cgcggccgtt ccctgtttgt cgttcgctac gccgacgatg ttgaagcgcg ttccgcgcac
      661 gtcgagctgc tgtccgatag cgccgtcgtg gctcccgaag aactcgaatg ccaaatcggg
      721 attgacgacg gcaacgtgcg cgtggctatg ttcgtcggct gccgtgagcg cgcggcccgc
      781 gagcacctga aatttcgaat ctccgccgta ccaggaccag ccgaaggcga cgaggtgttt
      841 agtttgccgg cgtacgttga agatgccgtg ctgcccgaac gacggggcgt agtaaggcgt
      901 cacgcgcgtt gccaaaccgc cgagcgcgtc ttccacgaca cgatagtctc gatagctcag
      961 ttgtgcgatt gcggggtcgg cttgctgcgg gtccggagcc gccgtgagcg agggattatc
     1021 gctcgaattg acgttcgaat taatcgcggc cgccgcgcct tcgctcagtc cgtataccga
     1081 gagcactgcg gcaacgccga tgagcatgcc gagcatcgtc aagatcgtac gaactttgtt
     1141 gcgcatgagc gcctctaacg cttgcgcgag atactccagc aggcggatca tcagaccggg
     1201 ggggttaggc gccgggcgtc gcggtggcgg acggcgattg cgcgacgacg gccgcgccgt
     1261 cgtgcagcag cgggttcttg tccgcaattc cgcgatcgcc ctcgcgaacg ccgctcagga
     1321 tcacggtctg cgtgtcgttc gaagccccgg tccggatcgc gcgtttgcga gccttgcctc
     1381 cggcgatgac gta
//
LOCUS       DASUCJ010000511         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_311170, whole genome shotgun sequence.
ACCESSION   DASUCJ010000511 DASUCJ010000000
VERSION     DASUCJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311170"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFU90_06055"
     CDS             <1..337
                     /locus_tag="VFU90_06055"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479375.1"
                     /translation="RNKGYALIVLTYLLQWLPWMKSPRITFAYHFYVDIPLICLCNAI
                     VLQRVWQWGTRQGTERGRWLGGLAAGGYAALALAAFVYFYPILAAHPISWNAWHQRMW
                     FGYWVIGPG"
     gene            407..1153
                     /locus_tag="VFU90_06060"
     CDS             407..1153
                     /locus_tag="VFU90_06060"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5479376.1"
                     /translation="MSGHSKWHNIKLKKGKVDAQRGALFTKLSKEIILAAKGGSADPE
                     ANYRLKMAVEKAREFNMPHDNIKRAIARGAGQTGEREIEEIRYEGYGPHGIAVVADAA
                     TDNRNRTAGELRFLFAKHGGTMGESGSVAWMFDAVGIVEADPAGDDEDRFTERALVDG
                     VQDVEFEPEFATVYTEPGRLASARDALRAAGVKISDAFLGMRPKNRIELQGEELAAGL
                     AFLEALDEHEDVQRVFGNLDASDVSMEALA"
     gene            1183..2229
                     /locus_tag="VFU90_06065"
     CDS             1183..2229
                     /locus_tag="VFU90_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU5479377.1"
                     /translation="MPRDWLFPIVLAIFVAVLVWFGRQIHDFLLPPSNMVTVPSFTGQ
                     TLTDANAEIGRLKLSSAGVDHTTSDRYPKNVVISQRPDAGEQVREGRQISFVISDGIV
                     TRLMPDLRYQSMREVALDLSRVRLRLAKVTYVKSDVIPEGHVISQDPAPLSSVAEGDP
                     VTLVVSRGGLSVVQVPNFGGMPIDDARALADKSGVKLGQIVWTPLGPNAPPHGTVVRQ
                     LPAPGGKIASYDPVSLQVSAGPFESGYIIRQVRVLVSVPAPDTSGDNTQSQQQQQQLD
                     VRLTVTDATGKYDLYHAFAEPGQKLDFTVTSVGTSLVDMYVNNQLVGETRLGTEPAVI
                     YGKKPASPAPKATK"
     gene            2226..2885
                     /gene="rpe"
                     /locus_tag="VFU90_06070"
     CDS             2226..2885
                     /gene="rpe"
                     /locus_tag="VFU90_06070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5479378.1"
                     /translation="MSAIKIAPSLLSADFARIAEQIADVQNAGADYLHLDVMDGRFVP
                     NITWGPKIIGDLRRLSPLVFDAHLMIVEPERYVADFRAAGCDRITFHLEATCHAQRLL
                     THVKSLGAKAGVAICPQTPVAMLQDIIEDCDVVLVMSVNPGFGGQRFIPRTFEKLREA
                     RALIERRGLTCAIEVDGGVGESNVRAIVDAGADELVMGSTIFGAADPGAELRRIRSLL
                     S"
     gene            2882..3853
                     /gene="thiL"
                     /locus_tag="VFU90_06075"
     CDS             2882..3853
                     /gene="thiL"
                     /locus_tag="VFU90_06075"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU5479379.1"
                     /translation="MKEDALVAAIRALVEPPIGRSIVLGIGDDAAVWQPSRSHRSVVT
                     SDMLVEGVHFTRALMTLEDAGWRAMTANASDVAAMGARPVLATVSLGMPEGTSEADVR
                     ELYRGIAGSSRAAGLNVVGGDLSRAPALTIAITAVGEVRASNVKTRAGGRPGAVLAVT
                     GALGAARAGLDAASGRIAIEGALAERALRAFRRPLARCAEGRYLGASANVQAMMDLSD
                     GLSTDLDRLCAASGCGASLENVPVAEAARAAAATAGSDPEHYALAGGEDFELLVAIAP
                     RAFGHLAARFRERFGRELLRVGTLRAQPGIEFRGERIERTGWEHFAT"
BASE COUNT          661 a         1219 c         1442 g          641 t
ORIGIN      
        1 gcgtaacaaa ggctacgcgc tgatcgtctt gacgtacctg ctgcagtggc tgccgtggat
       61 gaaatcgccg cgcatcacgt tcgcgtacca cttttacgtc gacatcccgc tgatctgcct
      121 gtgcaacgcg atcgtgctgc agcgcgtgtg gcagtggggg acgcgccagg gaacggagcg
      181 gggccgctgg ctgggcggcc tggccgccgg cggatacgcg gcgctcgcgc tggcggcgtt
      241 cgtgtatttt tatccgatcc tggcggcgca cccgatttcg tggaacgcct ggcaccagcg
      301 gatgtggttc ggttactggg tcatcggacc cgggtaggtt ttcccggcgc ccggcgtgcg
      361 ggaagttcgc gccggtgcgt gaatcggagc ttcccgagag gttgacatgt ccggacattc
      421 taagtggcat aacatcaagc tcaagaaagg taaagtcgac gcgcagagag gcgcgttgtt
      481 taccaagctg agcaaagaga tcattctggc cgccaagggc ggctcggcgg atcccgaggc
      541 caactaccgg ctgaagatgg cggtggaaaa ggcccgcgaa ttcaacatgc cgcacgacaa
      601 catcaagcgg gcgatcgcgc gcggcgccgg acagacgggc gagcgcgaga tcgaagagat
      661 tcgctacgaa ggctacggcc cgcacggcat cgcggtggtc gccgacgccg cgaccgacaa
      721 ccgcaaccgc accgccggcg aactgcggtt tctgttcgcc aagcacggcg gcacgatggg
      781 cgaatcgggc agcgtggcgt ggatgttcga cgcggtcggg atcgtggaag ccgatccggc
      841 cggcgacgac gaggaccgct tcaccgagcg cgcgctggtc gacggcgtgc aggacgtcga
      901 attcgaaccc gagttcgcga cggtctatac cgaacccgga cggctggcga gcgcgcgcga
      961 cgcgctgcgg gctgcgggcg tgaagatttc cgacgccttc ctcggcatgc ggccgaagaa
     1021 ccgcatcgag ctgcaaggcg aggagctggc cgcggggctc gcgttcctgg aggcgctcga
     1081 cgagcacgaa gacgtacagc gcgtcttcgg caatctcgac gcgagcgacg tgtcgatgga
     1141 ggcgctcgcc tgagaattac gcgcgacgcg attcgcgagt acgtgccgcg cgattggctg
     1201 tttccaatcg tgcttgcgat tttcgtcgcc gtcttggtgt ggttcgggcg gcagatccac
     1261 gattttctgc tgccgccgtc gaacatggtt acggtgcctt cgttcaccgg acaaacgctc
     1321 accgacgcca acgccgagat cggacgcctg aaactgtcgt ccgcgggcgt ggatcatacg
     1381 accagcgacc ggtatccgaa gaacgtcgtg atctcgcagc gccccgatgc cggcgaacag
     1441 gtgcgcgaag gccgccaaat ctcgttcgtg atcagcgacg ggatcgtgac gcggctgatg
     1501 cccgacctgc gctatcaatc gatgcgcgag gtcgcgctgg atttgtcgcg cgtacgcttg
     1561 cgccttgcca aggtgacgta cgtgaagagc gacgtgattc ccgagggaca cgtgatctcg
     1621 caggatccgg cgccgctgtc gagcgtggcc gagggcgatc cggtgacgct ggtggtgagc
     1681 cgaggcgggc tttcggtcgt gcaggttccg aacttcggcg ggatgccgat cgacgacgcg
     1741 cgcgccttgg ccgataagtc gggggtgaag ctcgggcaga tcgtctggac gccgctgggg
     1801 ccgaacgcgc cgccgcacgg cacggtggtg cggcagctgc cggcgccggg cggaaagatc
     1861 gcgtcgtacg atccggtctc gctgcaagtc agcgcggggc cgttcgagtc cggctacatc
     1921 attcggcagg tgcgcgtgct ggtgtcggtg ccggcgcccg atactagcgg cgacaacacg
     1981 caatcgcaac agcaacagca acagctcgac gtgcggctga cggttaccga tgcgaccgga
     2041 aagtacgatt tgtaccacgc gttcgccgaa ccggggcaga agctcgactt cacggtgacc
     2101 tcggtcggga cgtcgctggt ggacatgtac gtcaacaatc agctggtcgg cgaaacgcgc
     2161 ctgggcaccg agccggcggt gatctacggc aagaaaccgg cgtcgccggc gccgaaggct
     2221 acgaaatgag cgcgatcaaa atcgcgccgt cgctgctgtc ggccgatttc gctcgcatcg
     2281 ccgagcaaat cgccgacgtg cagaacgccg gcgccgacta tctgcacctg gacgtgatgg
     2341 acggacggtt cgtgccgaac atcacctggg ggcccaagat tatcggcgat ttgcgccggc
     2401 tctcgccgct ggtgttcgac gcgcacttga tgatcgtcga gccggaacgc tacgtcgccg
     2461 acttccgggc ggccggctgc gaccgcatca ccttccattt ggaagccacc tgccacgcgc
     2521 agcggctgct gacgcacgta aaatcgctgg gggccaaggc cggggtcgcg atttgcccgc
     2581 agacgccggt cgcgatgctg caagacatca tcgaagactg cgacgtcgtg ctggtgatga
     2641 gcgtcaatcc ggggttcggc ggacagcgat tcatcccgcg cacgttcgag aaactgcgcg
     2701 aggcgcgcgc gctgatcgag cggcgcgggc tcacctgcgc gatcgaggta gacggcggcg
     2761 tcggcgaatc gaacgtgcgc gcgatcgtgg atgccggcgc cgacgagctg gtgatgggat
     2821 cgacgatctt cggcgccgcg gatccgggcg cggagctgcg ccgcatccgt tcgctccttt
     2881 cgtgaaagag gacgccctcg tcgcggcgat ccgcgcgctc gtcgagccgc cgatcggccg
     2941 ttcgatcgtc cttggaatcg gcgacgacgc ggcggtctgg cagccgtcgc gctcgcatcg
     3001 cagcgtcgtc accagcgata tgctggtgga aggcgtgcac ttcacccgcg cactgatgac
     3061 gctcgaggac gccggctggc gcgcgatgac cgccaacgcg agcgacgtgg cggcgatggg
     3121 cgcgcggccg gtcctggcga cggtttcgct cggcatgccg gaagggacga gcgaagcgga
     3181 cgtgcgcgag ctgtatcgcg ggatcgcagg ttcgtcacgc gcggcggggt tgaacgtggt
     3241 gggcggcgac ttgtcgcgcg cgccggcgtt gacgattgcg attacggccg tgggcgaagt
     3301 ccgtgcgtcg aacgtgaaaa cgcgcgcggg cggccggccg ggagcggtgc tggcggtgac
     3361 cggcgcgttg ggggcggcgc gcgccggact cgacgcggca agcggacgga ttgcaatcga
     3421 aggagcgttg gcggaacgag ccttgcgcgc gtttcgccgt ccgctcgcgc gttgcgccga
     3481 agggcgctat ctcggcgcca gcgccaacgt gcaggcgatg atggatcttt ccgacgggct
     3541 gtcgacggat ctcgaccggc tgtgcgccgc gagcggctgc ggcgcgtcgc tcgaaaacgt
     3601 tccggtcgcg gaggcggcgc gcgccgccgc ggcgacggcc ggaagcgatc ccgagcacta
     3661 tgcgctggcg ggcggcgagg acttcgagct actggtcgcg atcgcgccgc gggcgttcgg
     3721 gcatttagcg gcgcgctttc gcgagcgctt cgggcgcgag ctgctgcgag tgggcacgct
     3781 gcgcgcgcag ccggggatcg aatttcgcgg cgagcggatc gaacgcacgg gctgggaaca
     3841 cttcgcaacg tgagcgatct gcataacggc ggtccgttcg aggtgcgtct cggcgacgag
     3901 agcggggcga acctgcgcgt cgagatcgcc gggtacgaac atcccgaggt agccgagccg
     3961 cac
//
LOCUS       DASUCJ010000512         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_479308, whole genome shotgun sequence.
ACCESSION   DASUCJ010000512 DASUCJ010000000
VERSION     DASUCJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479308"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..627)
                     /locus_tag="VFU90_06080"
     CDS             complement(55..627)
                     /locus_tag="VFU90_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5479380.1"
                     /translation="MRKPFFPIGLSVDGRTCVVIGAHDDREAIEKAAALRESGADVRR
                     IEDPQSLREEDVASAFFVISTPQDAALSKRLREYADRHRFLLCCIDQPAFGFVAMLAI
                     AKAGPVRVAISTAGLAPRVGKVLKLALQQAMDSTFVRFVERLAAERERVRAAHPSPSE
                     SELRRAAAIETASGFEADVRLKYPDWFRDG"
     gene            complement(639..1487)
                     /locus_tag="VFU90_06085"
     CDS             complement(639..1487)
                     /locus_tag="VFU90_06085"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU5479381.1"
                     /translation="MFRIVEAPAEVPAPPSIDVAVAADWLTAFLRDELILRRHIGRAV
                     VGLSGGVDSAVTAFLCARALGPDHVYAFRLPYVTSSPASLEDARLVIDALGIHSRTIE
                     ITAAVDGYLQFEPEADARRRGNVMARVRMAVLFDQSAKLDALPIGTGNKTERLLGYFT
                     WHADDTPPLNPLGDLFKTQVWALAAYLGVPQRLIDKAPTADLEAKQTDEADLGISYAR
                     ADAILAQLLLGYSDAQLIERGFAPADVALVRRRVDGTHWKRHLPTTAMLSGTAINEFY
                     LRPVDF"
     gene            complement(1471..>2024)
                     /locus_tag="VFU90_06090"
     CDS             complement(1471..>2024)
                     /locus_tag="VFU90_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5479382.1"
                     /translation="HRKMFLATYGVFDEERFLSRGRKLGVFDTRFGRMAMLVCEDAWH
                     AIVPTLAAVKGARILIVPSASPGRGIDGNGELTSVARWREMLVSAAVEHGIFVLYAGL
                     TGFEGGKGMSGNSCAIDPRGRVLVQAPANDAAIVRADLDLREIDLARAGLPLLGDLDA
                     VLPDLLLDEELPLPRAGTDVSNR"
BASE COUNT          351 a          699 c          650 g          324 t
ORIGIN      
        1 ccgagcagca gctccgttcc gacggcgatc agctcgacgc tcgccggacg ccgctcatcc
       61 atcccgaaac cagtcgggat acttcaaacg cacgtcggcc tcgaagccgc tagccgtttc
      121 gatcgccgcc gcccggcgca gctccgattc ggacggcgaa ggatgcgccg cccgtactcg
      181 ctcgcgttcg gcggccagcc gttcgacgaa ccgcacgaag gtcgagtcca tcgcctgctg
      241 cagggcgagc ttgagcacct tgccgacgcg cggcgccaaa ccggcggtgg aaatcgccac
      301 gcgcaccgga cccgccttgg cgatcgccag catcgcgacg aaaccgaagg ccggctggtc
      361 gatgcagcac agcaaaaagc gatggcggtc ggcgtattcg cgcaagcgct tcgagagcgc
      421 ggcgtcctgc ggcgtcgaaa ttacgaaaaa cgcgctcgcg acgtcctctt cgcgcaaact
      481 ttgcggatcc tcgatccgtc gcacgtcggc gccggactcg cgcagcgccg ccgctttctc
      541 gatcgcctcg cggtcgtcgt gcgcgccgat caccacgcac gtccgtccgt cgacgctcag
      601 cccgatcgga aaaaatggct tgcgcatccc cgcgatcgct aaaagtccac cggacgcagg
      661 tagaactcgt tgatcgcggt tccgctcagc atcgcggtcg tcggcagatg acgcttccag
      721 tgcgtgccgt cgacgcgccg ccgcacgagc gcgacgtcgg cgggagcgaa cccgcgttcg
      781 atcagttggg cgtcggagta gccgagcagc agctgcgcga ggatcgcgtc ggcgcgtgcg
      841 taggaaatgc cgaggtcggc ctcgtcggtt tgcttggctt cgaggtcggc cgtcggcgct
      901 ttgtcgatca gcctttgcgg cacgccgaga tacgcggcca gcgcccacac ctgcgttttg
      961 aagagatcgc cgagcggatt cagcggcggc gtgtcgtccg cgtgccacgt gaaatatccc
     1021 agcaggcgtt cggttttgtt cccggttccg atcggcagcg cgtccagctt tgccgattgg
     1081 tcgaagagaa cggccatccg gacgcgcgcc atcacgttgc cgcggcgccg cgcgtccgcc
     1141 tcgggctcga actgcaaata tccgtcgacg gcggcggtga tctcgatcgt acgcgagtga
     1201 atgccgagcg cgtcgattac cagccgtgcg tcctccaggc tcgccgggct cgacgtaacg
     1261 tacggaagcc ggaatgcata cacgtggtcg ggcccgagag cgcgcgcgca taagaaagcg
     1321 gttaccgcgg agtcgacccc gccggagagt ccgaccaccg cccggccgat atgccggcgc
     1381 agtatcagct cgtcgcgcaa aaaggcggtc agccaatccg cggctaccgc gacgtcgatg
     1441 gacggcggcg cgggtacttc cgccggcgct tcaacgattc gaaacatcgg ttccggctcg
     1501 cggcagcggc agctcttcgt cgagcagcag gtctggaagg acggcgtcca gatcgccgag
     1561 cagcggcagc ccggcgcgcg ccagatcgat ctcgcgcaaa tcgaggtcgg cgcgaacgat
     1621 cgccgcatcg tttgccggcg cctgcacgag cacgcgtccg cgcggatcga tggcgcacga
     1681 gtttccgctc atgcccttcc cgccttcgaa cccggtgagg cctgcgtaca acacgaagat
     1741 gccgtgttcg accgccgccg aaacgagcat ttcgcgccac cgcgcgacgc tggtcagctc
     1801 gccgttgccg tcgattcccc gtcccggcga agcgctcggg acgatcagta tccgcgcgcc
     1861 tttcaccgcc gcgagcgtcg gaacgatggc gtgccatgcg tcctcgcaaa cgagcatcgc
     1921 cattctgccg aagcgggtgt cgaagacgcc cagcttccga ccgcgcgaca aaaagcgttc
     1981 ttcgtcgaac acgccgtacg tcgcgagaaa cattttgcga tgaa
//
LOCUS       DASUCJ010000513         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1243607, whole genome shotgun sequence.
ACCESSION   DASUCJ010000513 DASUCJ010000000
VERSION     DASUCJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243607"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFU90_06095"
     CDS             <1..427
                     /locus_tag="VFU90_06095"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5479383.1"
                     /translation="IAGNGLWLTFYVILTIALTDIFGMVVGTLIGRHHLTKVSPSKTV
                     EGSFGALAVVTAFATAATWVPQLHLLWWQGLVLGLLTGIAAQAGDLVESALKRDAGVK
                     DTGAMIQGHGGVLDRFDSYLFGGVAFFATLHLLGVLQVN"
     gene            429..1583
                     /locus_tag="VFU90_06100"
     CDS             429..1583
                     /locus_tag="VFU90_06100"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5479384.1"
                     /translation="MARKRVAILGSTGSIGTQALDVIARHRERFEVVALAAGRNVELL
                     RHQAAEFAPKIATTVRDGPDGLMRAALESEPDVVLAATDGAVAFDAIFAAVERGIAIA
                     VANKELIVAAGELLVAAARESGARLLPVDSEHSAIFQCLVGEAPASVAEIVLTASGGP
                     FWRTSREEMERADLAAALAHPTWRMGTKNTIDSATMMNKGLEVIEASRLFGLPGSRVR
                     FVVHPQSIAHGFVIFTDGSVKGQMAAPDMRLPIGYALAYPDRLDAPPGSMPADGALTA
                     LGGAVGADGLRYDFEAPDPERFPCVRLAYEALEAGGTVPAVLSAANEVAVEAFVEGRI
                     AFGQIAGIIEGAMDGVPRGQPTLDGVRSADRAAREAANNLVESIERSPCR"
     gene            1586..2701
                     /locus_tag="VFU90_06105"
     CDS             1586..2701
                     /locus_tag="VFU90_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU5479385.1"
                     /translation="MVTLVGVGKVVTFIVMLSVLVVLHELGHFVMARRCGVRVNEFAV
                     GMGPKLFGWRSPRSGTLYSFRALPIGGYCAMQGEDGKTSEAEQQREFRETHAADGGNF
                     QAKSAWQRLAIVLAGPIANFVLCYVILLVGALAFGVAGSANQPVVGVVVKGSPAAVAG
                     LRAGDRIVAIDTAAVANGGTLVDTIHNSLGKRLDLVYERNGARTEVYVTPALCPIPGK
                     KWGCIGFSPVPAFQRVGPLEAIRVSGSEFGNIADETVGSIVLLATQFTKYAPQISGPI
                     GMGQVAATVEDWGWGPYFSLAATISFALGLFNLLPIPALDGGRAAFIIAELIRGKPVD
                     LEKEAMVHIAGFAALMALMLLVAFHDIARIASGNGVI"
     gene            2706..>2955
                     /locus_tag="VFU90_06110"
     CDS             2706..>2955
                     /locus_tag="VFU90_06110"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5479386.1"
                     /translation="MLRLRRRSKPIFLQNKTGKADAKGVWIGGDHPIVVQSMTTTDTA
                     DADKTLEQVYGLAMEGCEVVRVTVKDPPDAAGLPAIVYR"
BASE COUNT          471 a          898 c         1055 g          531 t
ORIGIN      
        1 gatcgccgga aacgggttgt ggctgacctt ctacgtcatt ctgacgattg cgctgacgga
       61 catctttgga atggtcgtcg gcacgttgat cggccggcac catctcacca aggtctcgcc
      121 gagtaagacg gtggaaggct cgttcggtgc gctggcggtc gtgacggcgt ttgcaaccgc
      181 cgccacgtgg gttccgcagc tgcacctcct gtggtggcag ggattggtac tcgggctgct
      241 gaccgggatc gcggcgcaag cgggcgacct ggtggaatcg gcgctcaagc gcgacgccgg
      301 cgttaaagat accggcgcga tgattcaagg gcacggcggc gtgctcgatc ggttcgattc
      361 gtatttgttc ggcggcgtcg cgtttttcgc aacgctgcac ttgctcggcg tcctgcaggt
      421 caactgagat ggcgcgcaaa cgggtggcga ttctcggctc caccggatcg atcggcacgc
      481 aagccctcga cgtgatcgcc cggcaccgcg agcgcttcga ggtggtggcg ctggcggcgg
      541 gccgcaacgt cgagctgctc cggcaccaag ccgccgagtt cgctccgaag atcgcgacga
      601 ccgtgcgcga cggcccggac ggcctgatgc gcgcggcgct ggaaagcgag cccgacgtcg
      661 tgctggcggc gaccgacggt gcggtggcgt tcgacgcaat ctttgcggcg gtcgaacgcg
      721 gcatcgcaat tgcggtcgcc aacaaagagc tgatcgtggc cgccggcgag ctgctggtgg
      781 ccgccgcgcg agagagcgga gcgcgtttgc tgccggtcga cagcgaacac agcgcgatct
      841 tccagtgttt ggttggggaa gcgccggcga gcgtcgcgga aatcgtgctg acggcgtcgg
      901 ggggtccgtt ttggcgtacg tcccgcgagg agatggaacg tgccgatttg gcggcggcgc
      961 tcgcgcaccc gacgtggcgg atgggaacga agaacacgat cgattcggcg acgatgatga
     1021 acaaagggct cgaggtgatc gaggccagcc ggctcttcgg attgcccggc agccgagtac
     1081 gtttcgtcgt gcacccgcag tcgatcgcgc acggctttgt gattttcacc gacggcagcg
     1141 tcaaagggca gatggcggcg cccgacatgc gcctgccgat cggctacgct ctcgcctacc
     1201 cggaccggct ggatgctccg ccgggctcga tgcccgccga cggcgcgctg acggccctgg
     1261 gaggcgccgt cggggccgac gggctgcgtt acgatttcga ggcgcccgat ccggagcgct
     1321 ttccgtgcgt ccgcttggcc tacgaggcct tggaagccgg cggaaccgtc ccagccgtcc
     1381 tctcggctgc aaacgaagtc gcggtcgagg cgtttgtcga aggtaggatc gcgttcgggc
     1441 aaattgccgg tatcatcgag ggagcgatgg atggcgtccc gcgcggccag ccgacgctcg
     1501 acggcgtgcg gtcggcggat cgtgcggccc gcgaagccgc gaacaatcta gtggaatcta
     1561 tcgaaaggtc gccgtgtcgc tagctatggt tacgttggtc ggggtcggga aggtcgtcac
     1621 gtttatcgtg atgctctccg tcctggtcgt gctgcacgaa ctcgggcatt tcgtgatggc
     1681 gcgccggtgc ggcgtcaggg tcaacgagtt tgccgttggg atggggccga agctgttcgg
     1741 gtggcgcagt ccccgcagcg gcacgctcta ctcgtttcgt gccctgccga tcggcggcta
     1801 ctgcgcgatg cagggtgagg acggcaagac gtccgaggcc gagcagcagc gcgaattccg
     1861 cgagacgcac gcggccgacg gcggcaactt ccaggcgaag tccgcatggc agcggctggc
     1921 gatcgtcctt gccggcccga ttgcgaactt cgtcttgtgc tacgtcatcc tgctggtggg
     1981 ggcgctggcg ttcggcgtag ccggcagcgc caaccagccg gtcgtcggcg tcgtcgtcaa
     2041 gggctcgccg gcggcggttg ccgggttgcg cgccggggat cgcatcgtcg cgatcgatac
     2101 cgccgccgtc gccaacggcg gaacgctggt cgacacgatc cacaactcgc taggcaagcg
     2161 cctcgactta gtctacgagc gcaacggcgc gcgcaccgaa gtgtacgtca ccccggcgct
     2221 ctgtccgatt cccgggaaga agtggggctg tatcgggttc tcgccggttc cggcctttca
     2281 gcgcgtcggc ccgctcgaag cgattcgggt gagtggatcg gagtttggaa acatcgccga
     2341 cgagacggtc ggcagcatcg tactgctggc aacgcaattc acgaaatacg cgccgcagat
     2401 ttcggggccg atcggcatgg gtcaggtcgc ggcgaccgtc gaggattggg gctgggggcc
     2461 gtacttttca ctggccgcga cgatttcgtt cgcgctcggg ctcttcaact tgctgccgat
     2521 ccccgcgctg gacggcgggc gcgcggcgtt catcatcgcc gaactgattc gcgggaagcc
     2581 ggttgatctg gagaaggaag cgatggtgca catcgccggc ttcgccgccc tcatggctct
     2641 gatgctcttg gtggcgttcc acgacatcgc ccgcattgcc agcggaaacg gggtaatcta
     2701 agaccgtgct acgcctgcga cgccgcagta aaccgatctt tctgcagaat aagaccggaa
     2761 aggccgacgc caaaggcgtg tggatcggcg gcgatcatcc gatcgtcgtg cagtcgatga
     2821 cgacgaccga tacggccgac gccgacaaga cgctcgagca ggtgtacggg ctcgcaatgg
     2881 aaggttgcga agtcgtgcgg gtgacggtga aggacccgcc cgacgccgcc ggactgccgg
     2941 cgatcgtcta tcgtt
//
LOCUS       DASUCJ010000514         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1136629, whole genome shotgun sequence.
ACCESSION   DASUCJ010000514 DASUCJ010000000
VERSION     DASUCJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136629"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VFU90_06115"
     CDS             <1..1518
                     /locus_tag="VFU90_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479387.1"
                     /translation="FVPVTSEPDRWTFLDNDLAHPVKGRVMPGDVFEFPDLPAHPSLT
                     ATSAVIGTRNYVRFAGVTAYLGGAGLPLGSYVVNFSPNQYFAQNSLTGANFDATWNLA
                     GSNNSLSALHLRYDSQNHTYLAFEQHFVGQHDYAIFSVNPLTKPERFFNLMLYEKLGQ
                     RFQVQTFTQFYVNQGVLLSAPPLAETQTTFVNATQAFPHSYLQLSSNFTNYNLLGPGS
                     LNPANYHPVAAGSLNHPSQMQLTFSSFQNRIGTSPFYEQIYGGYGFNHDSVGPNQYWY
                     PLQPSQVGLQYFGNTTYTTIWNTIFGFNLFMPSVKLGDRDNPYKTYYLNAVFNKQRQW
                     NSLPHHVDTTTTTFSLSRQFSRPVNSYLSYQIQNTGDYYLQGGYQPCTPQPNPQLSGY
                     CPPSFTSFRGVSTLRTLSLGINYDPNPEFNASLVGRHHDDFPIPVPDLFPLPLTNVLG
                     QPLYNNYLGQPPYDLTGDVRFKILPHMVLDVQRTYYFNFGNIRWSPYFIVQVLPE"
     gene            1515..2396
                     /locus_tag="VFU90_06120"
     CDS             1515..2396
                     /locus_tag="VFU90_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479388.1"
                     /translation="MSRLRVAFSIAVAALAIALTAAAPAIVVTGQLLAYQDGFVFFTT
                     GDGFRLAPGVAIRNADGSGPAAVAPAPRIWARAHFDPNGTVTELDLSRTKLPPEGNFA
                     DVARYAVALSSPIPNPDLMPATPAPGKPLVPSTQHFSGKPVLVTFTVQVPPNTAFSAS
                     VYITTDASGWNPQAIPMDRTDSLHYQITRRLNSGTVFRYLYDRGSFQAVETAENGLQR
                     EPRAIVVTDADVRVVRDTVYAWVDALQGGANGRQPNGLPTPYNPAPFPNLPGGFPSPG
                     IPTPHAGKPPAGFAKER"
     gene            complement(2383..2994)
                     /locus_tag="VFU90_06125"
     CDS             complement(2383..2994)
                     /locus_tag="VFU90_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479389.1"
                     /translation="MKFRRVTLLLVLAVAACSHAKTSSNPATPTAAPTPAPTPTARVR
                     PAPEPRPSAGGSSPSATGGPATPAPRLIVQPTPGPVTTPTGGAPAASAAPTATPQPES
                     SVPLLPPNAPPRIVAVDISETTVHSGDTVSGMVITSSNVASVEVRVATYGMSLAKIGV
                     GRFSLEYTVGSLPFFVHGTYQMRIIARNTRGDATQTTLPITVP"
     gene            3048..>3992
                     /locus_tag="VFU90_06130"
     CDS             3048..>3992
                     /locus_tag="VFU90_06130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5479390.1"
                     /translation="MLTVEPQASMNASPSQHPVLSVVVPLYDEAGNVAPLLARLTAIL
                     DRLPERPSYEIVLVNDGSADATADLVRAEMRVRPHIVLVNLSRNFGHQLAATAGIELA
                     AGDAVILMDGDLQDPPELVEAFVGKWREGYDVVYAVRRTRKGESAFKLITAGLFYRMI
                     KRLTKVAIPVDTGDFRLMSRRVVEALRRSPERHRFLRGMVSWVGYKQIGVPYDRDERR
                     SGTTKYPLPRMIRFAIDGITSFSDIPLRFASYFGFAVSGIAFVYALIVVIAKLFRVYP
                     PGYTPGWASTIVAVVFLGGVQLISLGIIGEYLGRIYDEV"
BASE COUNT          679 a         1393 c         1198 g          722 t
ORIGIN      
        1 ttcgttccgg taacgagcga gccggatcgc tggacgtttc tcgacaacga tctcgcgcat
       61 ccggtgaaag gccgcgtcat gccgggcgac gttttcgaat tccccgacct gccggcgcat
      121 cccagtctca ccgctacctc cgcggtgatc ggcacccgca actacgttcg ctttgcggga
      181 gtgaccgcgt acctcggcgg cgcggggctg cccctcggct cgtacgtcgt gaacttctcc
      241 cccaatcaat acttcgcgca gaactcgctg accggcgcga acttcgacgc gacctggaac
      301 ctcgccggca gcaacaactc gctgtcggcg ctgcacctgc gctacgactc ccaaaaccat
      361 acctatctgg cgttcgagca acacttcgtg gggcagcacg actacgcgat cttttcggtc
      421 aacccgctga ccaaaccgga acggttcttc aacctgatgc tttacgaaaa gctcgggcag
      481 cgcttccagg tccaaacgtt cacccagttt tacgtcaatc aaggcgtgct cttgtcggcg
      541 ccgccgctgg ccgagaccca gaccaccttc gtcaacgcga cgcaggcgtt tccgcactcg
      601 tacctccagc tcagctcgaa cttcaccaac tacaacttgc tgggaccggg ctcgctcaat
      661 ccggccaact accacccggt ggcggccggc agcctgaatc acccctcgca gatgcagttg
      721 accttctcga gctttcaaaa ccgcatcggc acctcaccgt tttacgagca gatctacggc
      781 ggctacggct tcaaccacga ttccgtcggt ccgaaccagt actggtatcc gctgcagccg
      841 tcgcaagtcg gcctgcagta cttcggcaac accacctaca cgacgatctg gaacacgatc
      901 ttcggtttca acctcttcat gccgtcggtg aaactcggcg accgcgacaa cccctacaag
      961 acgtattacc tcaacgccgt attcaacaaa cagcggcagt ggaactcgct tccgcaccac
     1021 gtcgatacga cgaccacgac cttcagcctg agccgccagt tctcgcgccc cgtgaactcg
     1081 tatctcagct accaaataca gaacaccggc gactactact tgcagggcgg ctaccagccg
     1141 tgcacgccgc agccgaaccc gcagcttagc ggttattgcc cgcccagctt cacgtcgttc
     1201 cgcggcgttt cgacgctgcg cacgctgtcg ctggggatca actacgaccc gaacccggag
     1261 ttcaacgcct cgctggtcgg ccgccatcac gacgactttc cgattcccgt accggacctg
     1321 tttccgctgc cgctgaccaa cgtgctggga cagccgctct acaacaacta tctggggcag
     1381 ccgccctacg atctcaccgg cgacgtacgt tttaagatcc tgccgcacat ggtgctcgac
     1441 gtccagcgca cctactactt caatttcgga aacatccgct ggagcccgta cttcatcgtg
     1501 caggttctgc ccgaatgagc cgcctgcgcg tcgcgttttc gatcgcggtc gcggcgctcg
     1561 cgatcgcgct gacggccgcc gctccggcca tcgtggtaac gggccagctg ctcgcctacc
     1621 aagacggctt cgtcttcttc accaccggcg acggattccg gctcgcgccc ggcgtggcga
     1681 ttcgcaacgc cgacgggagc ggacccgccg cggtggcgcc cgcgccgcgc atttgggcgc
     1741 gcgcgcactt cgatccgaac ggaaccgtga ccgagctgga tctttcgcgg acgaagcttc
     1801 cgccggaagg aaacttcgcc gacgtcgcgc gctacgcggt cgccctctcg tcgccgattc
     1861 ccaaccccga cctcatgccg gcgacgcccg cgccggggaa accgctcgtc ccgtcgacgc
     1921 agcatttttc gggaaagccg gtgctggtga cgttcacggt gcaagtgccg ccgaacaccg
     1981 cgttctcggc cagcgtgtac atcacgaccg acgccagcgg ctggaacccg caggcgattc
     2041 cgatggaccg caccgattcg ctgcactatc aaatcacgcg ccgcctcaac tccggtaccg
     2101 tgttccggta tctctacgac cgcggttcct tccaagcggt cgaaaccgcg gaaaacggtc
     2161 tgcagcgcga gccgcgtgcg atcgtcgtca ccgacgccga cgtgcgcgtc gttcgcgaca
     2221 ccgtgtacgc ttgggtcgac gcgctgcaag gcggtgcgaa cggccgccag cccaacggct
     2281 tgcccacgcc gtacaatccc gcgcccttcc ccaacctgcc cggcggtttc ccgtcgccgg
     2341 gaatcccgac gccgcacgcg ggcaaaccgc cggccggctt cgctaaggaa cggtaatcgg
     2401 cagggtcgtc tgggtcgcgt cgccgcgcgt gttgcgcgcg atgatccgca tctgatacgt
     2461 gccgtgtacg aagaacggca gcgatccgac cgtgtactct aaggagaacc ggccgactcc
     2521 aatcttggcc agcgacattc cgtaggtggc gacgcgcacc tcgacgctcg cgacgttcga
     2581 actcgtgatc accatccccg aaacggtgtc gcccgagtgc accgtcgttt cgctgatgtc
     2641 gaccgcgacg atccgcggcg gtgcgttggg cggaagcagc gggacgctcg actcgggctg
     2701 cggcgtcgcg gtcggcgccg cactggctgc gggtgcccct ccggtggggg tagtcaccgg
     2761 tcccggcgtc ggctgtacga tcaggcgcgg cgcgggggtt gcgggcccgc cggtggcgct
     2821 cggcgagctc ccgccggccg acggccgcgg ctccggagcc ggacgaacgc gcgcggtggg
     2881 ggtgggcgcc ggcgtcggtg cggcggtcgg cgttgccggg ttcgacgacg tcttggcatg
     2941 gctgcacgcg gcaaccgcga gaacgagcag cagcgtaacc cggcgaaatt tcacaagccg
     3001 ccaggttctc gaccgaaacg gcggttccgc ttgacgcgag gcgggcggtg cttaccgtcg
     3061 aaccgcaagc gagcatgaac gcttcgccct cgcagcatcc ggtgttgagc gtcgtcgttc
     3121 cgttatatga cgaagccggc aacgtcgcac ccttgctcgc gcgcctgacg gcgattctcg
     3181 accggctgcc ggaacggccg agttacgaaa tcgtgctggt caacgacggc agcgccgacg
     3241 cgaccgccga cctcgttcgc gccgaaatgc gcgtccgtcc gcatatcgtt ctcgtcaact
     3301 tgtcgcgcaa cttcggacat cagctggcgg cgaccgccgg catcgagctc gccgccggcg
     3361 acgcggtgat cctgatggac ggcgacttgc aggatccgcc cgagctggtc gaggccttcg
     3421 taggcaagtg gcgcgagggg tacgacgtcg tgtacgcggt ccgtcgcacg cgcaagggcg
     3481 agagcgcgtt caagctcatc accgccgggc tcttctatcg tatgatcaaa cgtttgacga
     3541 aggtcgcgat cccggtcgac accggcgatt tcaggcttat gagccggcgc gtggtcgagg
     3601 cgttgcggcg ttcgcccgag cggcatcgct ttctgcgcgg catggtgagc tgggtcggct
     3661 acaaacaaat cggcgtgccg tacgaccgcg acgagcggcg ctcgggtacc accaagtacc
     3721 cgcttccgcg aatgatccgc tttgcgatcg acggtatcac gtcgttctcc gacatcccgc
     3781 tgcggtttgc gtcgtatttc ggctttgcgg tcagcggcat cgcgttcgtc tacgcgctga
     3841 tcgtcgtgat cgcgaagctg tttcgcgtct atccgcccgg ttacacgccg ggctgggcgt
     3901 cgacgatcgt cgcggtcgtg tttctcggcg gcgtacagct gatcagcctc ggcatcatcg
     3961 gcgagtacct cggcagaatc tacgacgaag ta
//
LOCUS       DASUCJ010000515         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_204335, whole genome shotgun sequence.
ACCESSION   DASUCJ010000515 DASUCJ010000000
VERSION     DASUCJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204335"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFU90_06135"
     CDS             complement(<1..245)
                     /locus_tag="VFU90_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479391.1"
                     /translation="MLEFDAALIERYGGSGPRYTSYPTADRFHAGYTSADYAREIAKR
                     NERASTESLSLYVHLPFCSSLCFYCACNKIITRNRGRS"
     gene            375..2711
                     /locus_tag="VFU90_06140"
     CDS             375..2711
                     /locus_tag="VFU90_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5479392.1"
                     /translation="MRQLAGFLSVVKHLPLVLSLAALLPSLATAQAPGGDATLPTVNV
                     IGTSPLIGSGIDRDAIPAQTGVLSSADLSRSGTPNMIGALNGQVGGAALGEAAGNPFQ
                     PTFFYHGFAASPLQGTTEGLAVYVNGMRFNQPFGDTVDWELIPDIAIDRLDILGSNPV
                     FGLNALGGALNVRLKDGFGFHGFEGDVSGGSFQQAQGEFQFGTRSGNTAAYVAGNAVH
                     QTGWRDLQSSSIRNLYGDIGWRGDSADVHLNLTYASSNLNGPGTSPVELLQADRKAQF
                     TAPNEVANEYAAAGLTGNVDLTGRLSLQVLAYYRHFRQNVTNGNVANDAPCNDGSGLL
                     CSDVGVVSTTAGGVPIPDFLAGGPYSQLDVQKTVTDAYGASVQITHAGELFGLRNHFV
                     GGVSFDAARNDFTARSSIGGLTLDSREFVGPGIVLDEPGTSVPVSARVTNATRGAYVA
                     DTLSLTARAAVTLSGRYNAAEVDLHDNNGGDLSGNHRYDHFNPAAGFTYRFARSLTAY
                     AGYAIANRTPTPAELSCAGPQNSCSLANFFVGDPHLDQVIAHTVETGVRGTLTPFAGA
                     DVKYELALFQTDLDDDIAFVNSVTQGRAYFANIGRTQRRGVDARVSLVTDRWNAYATY
                     ASIQARFRDGFIEGSGDNPMAGADGDITVRSGDRLPGTPAQQLKLGAHYKVTDRWTVG
                     LIGLASTGAYLFGDEANLTPRLPGYYTLNASTTYQLTKHVQLFAWIDNLTNQKYYTFG
                     TFSPTSSVFLVQAPNATNPRAYSPAAPIGGFVGMRLTY"
     gene            complement(2712..>3167)
                     /locus_tag="VFU90_06145"
     CDS             complement(2712..>3167)
                     /locus_tag="VFU90_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HEU5479393.1"
                     /translation="HLLLMVVAASTLGIGAASAHNNAAHSDESAFGHPGDPARVARTI
                     EVSMTDAMRFTPAAITVRKDETVRFVVRNDGRLVHEMVLGTDQDLREHAAMMREHPGM
                     AHAEPNMVRVEPGAKAQMVWTFDKAGRFTFACLVPGHFEAGMKGKVEVD"
BASE COUNT          527 a         1107 c          978 g          555 t
ORIGIN      
        1 gaacgcccgc ggttgcgggt gatgatcttg ttgcaggcgc agtagaagca gagcgagctg
       61 cagaacggaa ggtggacgta gagcgacagc gactccgtcg acgcgcgctc gttgcgcttc
      121 gcgatctcgc gcgcgtagtc cgccgacgtg tatccggcat ggaagcggtc ggctgtcgga
      181 taggacgtat agcgcggtcc cgatccaccg tatcgctcga tcagcgcagc gtcgaattcc
      241 agcatcgccg atcttgccgg tcgcgtgtca ccgtccaagg gatctctccg ccgcctgcgt
      301 catgcgattt gacttcggcc ttctcatgca atttggcatg gaatatgcca gcatgcgcgc
      361 tgccccgcct tgacatgcgt caactggcgg gattcctgtc cgtcgtgaag cacctgccgc
      421 tcgtcctctc gctcgccgcg ctcctgccgt cgctcgccac cgcgcaggcg ccgggtggtg
      481 acgccactct tccgacggta aatgtgattg gcacgtcgcc actgatcggt tcggggatcg
      541 accgtgacgc gattcccgcg cagaccggcg tgctgtcgag cgccgacctc tcgcgcagcg
      601 gaacccccaa catgatcggc gcgctcaacg ggcaggtcgg gggtgctgcg ctcggcgaag
      661 cggccggcaa cccgttccag ccgacgttct tctaccatgg gttcgccgct tcgccgctgc
      721 agggcaccac ggaaggactg gccgtctacg tcaacggcat gcgcttcaac cagccgttcg
      781 gcgatacggt cgactgggag ctcatccccg acatcgcaat cgaccggctc gacatcctcg
      841 gctcgaaccc cgtattcgga ctgaatgctc tcggcggcgc gctcaatgtg cgtctcaagg
      901 atggcttcgg cttccatggt ttcgagggcg acgtgtcggg cggctccttc cagcaggcgc
      961 agggcgagtt ccagttcgga acgcggagcg gcaacaccgc cgcgtacgtc gcgggcaacg
     1021 ccgtccacca gacgggctgg cgcgacctcc aatcctcgtc gatccggaat ctgtacggtg
     1081 atatcggatg gcgcggcgat tccgccgacg ttcatctcaa tctgacctac gcgagctcaa
     1141 atctcaacgg tccgggcacg tcgccggtcg aactcctgca ggccgaccgc aaggctcagt
     1201 tcactgcgcc gaacgaggtc gcgaacgagt atgccgcagc gggactcacc ggcaacgtcg
     1261 atctcaccgg tcgcctttcg ctgcaggtgc tcgcctacta tcgtcacttc cggcaaaacg
     1321 tcaccaacgg caacgtcgcc aacgatgcgc cgtgcaacga cggctcgggc ctgctgtgct
     1381 cggacgtcgg ggtcgtcagc acgaccgccg gtggcgtgcc gatccccgat ttcctcgccg
     1441 gcggcccgta ttcgcagctc gacgtgcaga agaccgtcac cgacgcctat ggcgcgtcgg
     1501 tgcagatcac gcatgccggg gaactgttcg ggctgcgcaa tcacttcgtc ggcggcgtga
     1561 gcttcgacgc cgcacgcaac gacttcaccg cgcgctcgtc gatcggcggc ctcacgctcg
     1621 atagccgcga gttcgtcggc cccggcatcg tgctcgacga gcccggcacc agcgttccgg
     1681 tttccgcgag agtgaccaat gcaacccggg gcgcctacgt cgccgacacc ctcagtctga
     1741 ccgcccgggc ggcggtaacg ctttccgggc gctacaacgc agcagaggtc gatctccacg
     1801 acaacaacgg cggcgatctc tctggcaacc atcgctacga tcacttcaac ccggcggccg
     1861 gattcaccta ccgcttcgcg cggtcgctga cggcgtacgc gggctatgcg atcgccaacc
     1921 gcacgccgac gccggcggag ctctcctgcg cggggccgca gaactcgtgc agcctcgcca
     1981 acttcttcgt cggcgatccg catctcgacc aggtgatcgc gcacacggtc gaaaccggcg
     2041 tgcgcggcac gctgacaccg ttcgccggcg cggacgtgaa gtacgagctc gcgctctttc
     2101 agaccgatct cgatgacgat atcgcattcg tcaacagcgt gacgcaggga cgcgcctact
     2161 ttgccaacat cggccggacg cagcgccgcg gcgtggacgc gcgcgtcagt ctcgtgaccg
     2221 atcgctggaa tgcctatgcc acgtacgcgt ccatccaggc gcggttccgc gacggcttca
     2281 tcgagggctc gggcgacaac ccgatggccg gcgccgacgg cgacatcacc gtgcgatcgg
     2341 gcgaccggct gccgggcacc ccagcgcagc agttgaagct cggggcccac tacaaggtta
     2401 ccgaccgctg gaccgtcgga ctcatcggcc tcgcatcgac cggtgcgtac ctgttcgggg
     2461 acgaggccaa cctgacgccg cgcctgccgg gctactacac gctcaacgcc agcacgacat
     2521 accagctcac gaagcacgtc cagctcttcg cgtggatcga caacctcacc aaccagaagt
     2581 actacacgtt cggtacgttc tcgccgacct cgtcggtgtt cctggtccag gcgccgaacg
     2641 cgacgaaccc tcgcgcctac agcccggccg cgccgatcgg cggctttgtc ggcatgcgcc
     2701 tcacgtattg atcaatccac ctcgactttg cctttcatgc cggcctcgaa gtgtccggga
     2761 acgagacagg caaaggtgaa gcggccggct ttgtcgaagg tccacaccat ctgtgccttc
     2821 gctcccggct cgacgcgcac catgttcggt tcggcgtggg ccatcccggg gtgctcgcgc
     2881 atcatcgcag cgtgctcgcg cagatcctgg tcggtgccga gcaccatctc gtgcacgagt
     2941 cggccgtcgt tgcgcacgac gaaacgcacc gtctcatcct tcctgacggt gattgccgcg
     3001 ggtgtgaagc gcatcgcgtc agtcatgctc acttcgatgg tccgcgcgac cctcgcggga
     3061 tcgcccggat ggccgaacgc gctctcgtcg ctatgtgcgg cattgttgtg cgcagaggct
     3121 gcgccgatgc cgagcgtcga cgccgcgaca accatcaaca gcaggtg
//
LOCUS       DASUCJ010000516         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1442227, whole genome shotgun sequence.
ACCESSION   DASUCJ010000516 DASUCJ010000000
VERSION     DASUCJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442227"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VFU90_06150"
     CDS             complement(<1..1589)
                     /locus_tag="VFU90_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479394.1"
                     /translation="MRRGVAGRFAGVAAAAAIFGSAAALAQPASPPPSTPPPLTYPSP
                     LPTSAASPGAAPAQSAAPQAPSAAPTPPPITVTPDNASVPIGGSTRLSVGSVLAPIAA
                     TARDPSLVDVAVDQPTQTITLAGKAPGSTVVTVSDARCLRRDVPVRVAYFAGSVPQQL
                     TLSITGDPASEDYVRREVAAAIVRSAQVRPGAQAVVGPDDVPFHGELGQDRVSVLSVP
                     VLLQGSAYVEVDSTIRVDVRNVAAPRISPDSLMVSDYPERLEENGLLFTADLRSEQPS
                     RFLYFHYNPPGQPNRRIVLRAQNLSPQPANVQFISGRGGPSPNEMEAGHVATKAFLIN
                     VVQNQGRLITIPGNTSLNVVEQDIPAGNVSCNLLQLRLLSGGSVQLTLFAQPADVDPN
                     EPLGSGGSLLEGTHPHARGIYPIPEFHYATQWYVDGEYLELPIGQIPLPNDLQGQALA
                     GDYGVLQSFVVTMQNPLSTPQAVAIYENPRGGRATGTYLIDGVLVQSHQVPPFSRYKI
                     RQYVVPARGFVRVTIVTMPEAG"
     gene            complement(1582..3219)
                     /locus_tag="VFU90_06155"
     CDS             complement(1582..3219)
                     /locus_tag="VFU90_06155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEU5479395.1"
                     /translation="MDLRASLRANFGFEGFYPGQEEVVSRVLRGQDTLAILATGAGKS
                     LTYQLPALMLEGTTVVVSPLIALMKDQLDMLRERGIEAVVAINSTLSEEQEVRARERI
                     RSGAIKIAYTTPEKLEDEAFLSLLQSIRVPLFVVDEAHCISQWGHDFRPAYLALGGAI
                     KKLGEPTVLALTATATPSVREDILRQLGIEDVKPIVRGFDRPNLIYEARKADKEADKV
                     KILERLFTGSDALEGTGIIYTATIKNALEVQSYLSQHLNMPAAVYHSKLHKEDRTSVQ
                     NVFMSETIRAVVATNAFGLGIDKPNIRFVVHYDLPGSLEAYTQEAGRAGRDGLPSRCV
                     LIYRMSDTRVQNYFLTGKYPDVEEVQRVFGTIEVFGEQAGGVAMTDLRKILQLPLTKL
                     KVILALLKKSGYIETSGKSAYGLTEAVRKNRSLVLSLANYETKKSYDQSKLAMMLQYA
                     ETTSCRRRFILNYFGEDFDVANCGACDNCLRAIRRGVGEGASTGGFKISDVVLHPKFG
                     VGTVERTERDLVTVLFPNVGYKTLLASAVRRSEAKIA"
     gene            3375..>4376
                     /gene="dnaX"
                     /locus_tag="VFU90_06160"
     CDS             3375..>4376
                     /gene="dnaX"
                     /locus_tag="VFU90_06160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5479396.1"
                     /translation="MSLYRTWRPRSFSDLVGQDAVVRTLTAAIEGGKLAHAYLFSGPR
                     GSGKTSAAKILARCIDCVNGPTATPDNSCENCLAILSGTALDVLEIDAASNRGIDEIR
                     ALRDAVKFAPSSMRMKVYVIDEAHMLTKEGANAFLKTLEEPPPHAVFILATTEPEKLP
                     VTILSRCQRYAFRRIAIPVMIAKMQEIARAEGIAADEGALAAIAYRADGGLRDALTML
                     EQAAAFAGGERITPQILDRAFGATGREFAEAVLDATVARDAAGALAAIERASDAGADM
                     HVLTRALIAAFRNALVARVDPDLLARDLAPEDAQRAAERAKVLPQAAGVRALRLLTDA
                     "
BASE COUNT          752 a         1406 c         1449 g          769 t
ORIGIN      
        1 cccgcttccg gcatcgtgac gatcgtaacg cgcacgaagc cgcgcgccgg cacgacgtac
       61 tggcggatct tgtagcgcga gaacggcgga acttgatgcg actgtacgag cacgccgtcg
      121 atcagatacg tgccggtcgc gcggccgccg cgcggatttt cgtaaatggc gaccgcttgc
      181 ggcgtcgaca gcgggttctg catcgtcact acgaacgatt gcagcacgcc gtagtcgccg
      241 gccagcgcct ggccctgcag gtcgttggga agcggaatct gaccgatcgg cagctccaag
      301 tattcgccgt cgacgtacca ttgcgtcgcg taatgaaact ccggaatcgg atagatgccg
      361 cgcgcgtgcg ggtgcgtacc ttcgagcagc gagccgccgc tcccgagcgg ttcgttggga
      421 tcgacgtcgg ccggctgcgc gaacagcgtg agctgtaccg aaccgccgga gagcagccgc
      481 agctgcagca ggttgcacga gacgttgccg gccgggatgt cttgttcgac cacgttcagc
      541 gacgtattgc cgggaatcgt tatcaaacgt ccctggttct gcaccacgtt gatgagaaac
      601 gccttggtcg cgacgtgccc cgcttccatt tcgttgggcg acggaccgcc gcgtccgctg
      661 atgaactgca cgttggccgg ctgcggcgag agattctggg cgcgcagcac gatgcgccgg
      721 ttcggctggc ccggcggatt atagtgaaag tagaggaaac gcgagggctg ttcgctgcgc
      781 aagtcggccg tgaacagcaa gccgttttct tccagccgtt cggggtaatc gctcaccatc
      841 agtgagtcgg gagaaatgcg cggcgcggcg acgttgcgca cgtcgacccg gatcgtgctg
      901 tcgacctcga cgtacgcgct tccctgaagg agaacgggta ctgaaagcac gctgacgcgg
      961 tcttgcccga gctcgccgtg gaagggcacg tcgtcgggtc ccaccacggc ttgcgcgccg
     1021 gggcgcacct gcgccgaacg gacgattgcg gcggccactt cgcggcggac gtagtcttcg
     1081 gaagcgggat ctccggtgat cgaaagcgtc agctgctgcg gcacgctgcc ggcaaaatag
     1141 gccacgcgca ccgggacgtc gcggcgaagg cagcgcgcgt cggagaccgt gacgacggtc
     1201 gagccgggcg ccttgccggc gagcgttatc gtttgcgtcg gctgatcgac cgcaacgtcg
     1261 accagcgagg ggtcgcgggc ggttgcggcg atcggcgcga gcaccgagcc gacgctcaaa
     1321 cgtgtcgagc cgccgatcgg aaccgacgcg ttgtcgggcg taacggtaat cggcggtggg
     1381 gtaggagcgg cggagggagc ttgcggggcg gcgctttgag ccggagccgc gcccggcgat
     1441 gcagcggagg tgggaagcgg gctcggatag gttaacggcg ggggcgtaga cggcggcggg
     1501 gaggcgggtt gcgcgagggc ggcggcgctg ccgaaaatcg cggccgcggc cgcgacgccg
     1561 gcgaaccgtc cggcgacccc tctacgcaat cttcgcttcg gaacggcgaa ccgccgatgc
     1621 cagcagcgtc ttatacccca cgttcggaaa gagcacggtc acgagatcgc gttccgtgcg
     1681 ttcgacggtg cccactccga acttcggatg cagaaccacg tcggagattt tgaatcctcc
     1741 ggtcgacgct ccctcgccga ccccgcgccg gatcgcgcgc aggcagttat cgcacgcgcc
     1801 gcagttggcg acgtcgaaat cctcgccgaa atagttgagg atgaagcgcc ggcggcacga
     1861 agtcgtttcg gcgtactgca gcatcatcgc gagcttgctc tgatcgtacg acttcttggt
     1921 ttcgtaattg gccaggctca agacgagcga acgattcttg cgcacggctt cggtgagacc
     1981 gtacgcggat ttgccggagg tctcgatgta gcccgacttc ttgagcagcg cgaggatcac
     2041 tttgagcttc gtgagcggca gctgaaggat tttccgcaga tcggtcatcg ccacgccgcc
     2101 ggcctgctcg ccgaagactt cgatcgttcc gaaaacgcgc tggacctctt cgacgtcggg
     2161 atacttgccg gtaaggaagt agttttgcac gcgagtgtcg ctcatccggt agatgagcac
     2221 gcagcgcgac ggcagcccgt cgcggccggc gcggccggct tcttgcgtat acgcctcgag
     2281 cgaccccggt agatcgtagt gcaccacgaa ccggatgttc ggtttgtcga tgcctagccc
     2341 gaaggcgttg gtcgcgacca ccgcgcggat cgtttcgctc atgaagacgt tctgaaccga
     2401 ggtgcggtct tctttgtgca gcttcgagtg gtagacggcg gccggcatgt tcaaatgctg
     2461 cgacagatag ctctgcactt cgagcgcgtt cttgatcgtc gcggtataaa tgatgccggt
     2521 gccttcgagc gcgtcgctcc cggtaaacag gcgctcgaga atcttgactt tgtcggcctc
     2581 cttatcggcc ttgcgcgctt cgtaaatgag gttgggacga tcgaacccgc ggacgatcgg
     2641 cttgacgtcc tcgatgccga gctgccgcag gatgtcctcg cggaccgacg gcgtcgcggt
     2701 agccgtcagc gcgagcaccg tcggctcgcc gagtttcttg atcgctccgc cgagcgccag
     2761 ataggcgggg cggaaatcgt gcccccactg gctgatgcag tgcgcttcgt cgaccacgaa
     2821 cagcggcacg cggatcgatt gcagcaagct caaaaaagct tcgtcttcga gtttttcggg
     2881 cgtcgtgtac gcgatcttga tcgcgccgga gcgaatgcgc tcgcgcgcgc gcacctcttg
     2941 ctcctccgag agcgtcgagt tgatggcgac gaccgcttcg atgccgcgtt cgcgcagcat
     3001 gtcgagctgg tctttcataa gcgcgatcag cgggctgacg acgaccgtcg tcccttccag
     3061 catcagtgcc gggagctgat aggtgaggct cttccccgca ccggtcgcga gaattgccag
     3121 cgtatcctgg cctcgcaaga cccgggatac gacctcctct tgacctgggt aaaacccttc
     3181 gaacccgaag ttggctcgca gcgacgcgcg caggtccacg aaaacccttt cgtccatcct
     3241 ttccggccgg gaactccaaa cgccggagct tccattgcgg cgccccagca ttgcctttaa
     3301 ccgttcccgt tccgaacccg tttcactcta ccccgcggca ggcgcgccca aacgctcctc
     3361 gtaagtcccc gggagtgtcc ctctaccgaa cgtggcgacc aaggtcattt tccgacctcg
     3421 tgggccagga tgccgtcgtg cgaaccctga ccgccgccat cgagggcgga aaactcgcac
     3481 acgcctactt gttctcaggt cccaggggtt ccggaaaaac ttccgccgcg aagattctgg
     3541 cgcgctgcat cgactgcgtc aacggcccga ccgctacgcc cgacaatagc tgcgaaaact
     3601 gcctggcgat cctctccggg acggcgctgg acgtgctcga gatcgacgcg gcgagcaacc
     3661 gcggcatcga cgagatccgc gcgctgcgcg acgccgtcaa gtttgcgccg tcctcgatgc
     3721 gcatgaaagt gtacgtcatc gacgaagcgc acatgctgac caaggaaggc gccaacgcgt
     3781 ttctcaagac gctcgaagag cctccgccgc acgccgtgtt cattctggcc accaccgagc
     3841 cggagaagct tccggtaacg attttgtcgc gctgccagcg gtacgcgttt cgccgcatcg
     3901 ccattccggt gatgatcgcc aaaatgcagg aaatcgcgcg agccgagggg attgcggccg
     3961 acgagggcgc gctcgccgca atcgcctacc gggccgacgg cggtctgcgc gacgcgctga
     4021 cgatgctcga gcaggccgcg gcattcgccg gcggcgagcg gatcacaccg cagatactcg
     4081 atcgtgcctt cggcgcgacc ggtcgcgaat ttgccgaagc ggtgctcgac gcaaccgtcg
     4141 cgcgcgacgc cgccggcgcg ctggccgcga tcgaacgggc gagcgatgcc ggcgcggaca
     4201 tgcacgtgct gacgcgcgcg ctgatcgccg cctttcgcaa cgcgctggtg gcccgcgtcg
     4261 atccggacct gctggcccgc gacctcgctc cggaagacgc ccagcgcgcg gccgaacgcg
     4321 cgaaggtgct gccgcaggcc gccggggtgc gcgccctgcg actgctcacc gacgcc
//
LOCUS       DASUCJ010000517         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_173503, whole genome shotgun sequence.
ACCESSION   DASUCJ010000517 DASUCJ010000000
VERSION     DASUCJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173503"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..385
                     /locus_tag="VFU90_06165"
     CDS             128..385
                     /locus_tag="VFU90_06165"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEU5479397.1"
                     /translation="MEITWKPVRRPDEARSALPTSAFAFPAERKEPLIDATHVRSAVA
                     RFAQVTGVSDGERAQAFANIKAAAAYYGVTVGAKNWHDLVG"
     gene            456..>1109
                     /locus_tag="VFU90_06170"
     CDS             456..>1109
                     /locus_tag="VFU90_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479398.1"
                     /translation="MVLRVEGDAADGAVESWLERLDAAGAGPTPSRTHVRYVVTRREI
                     YYVPRIEIEAYAASVLRDGTLSSLRPVDILNTGKLGSTSVTPADRTIARLAGVSGAVR
                     PSLSSPSPLILATLLEAAIAVGRLYWQSASNPALQRQAIETGRIVWGTGEDGRQRPRI
                     AGRPHAVLLACNPVWYVDPVTMRAGPVDLGIAPDLAAAAVSAPALTARQAERSQIAWR
                     "
BASE COUNT          186 a          337 c          395 g          191 t
ORIGIN      
        1 ggacaggttc gtacgaccga agagatcgtc gacgctctgg cctcgcgggg gcgcaatccg
       61 taacgcgcaa tcgatcgtcc atcgttcggt cgcgcttcgg acatcgccgg cacgtacgtt
      121 cgaaggcgtg gagattactt ggaaacccgt acgccggccc gacgaagcgc gttcggcgtt
      181 gccaacaagc gcgtttgcct ttccggcgga gcgcaaggag ccgctgatcg acgccacaca
      241 cgtgcgcagc gccgtagcgc gcttcgctca ggttaccggc gtcagcgacg gcgagcgcgc
      301 tcaagcgttt gcaaacatca aggcggcggc agcatactac ggagtaaccg tcggcgcaaa
      361 gaactggcac gacttggtgg gatgagccca agctcgctca cgcgtgaggt tcgaaagagt
      421 gtatccggag gcctcgcgtc gtatgtagac gggttatggt tttgcgggtt gagggcgacg
      481 ccgccgacgg tgcggtcgaa agctggctcg agcggctcga cgccgcgggc gcggggccga
      541 cgccgagccg gacgcacgtg cggtacgtcg tcacgcgtcg cgagatttac tacgttcccc
      601 ggattgaaat cgaggcgtac gcggcgagcg tcttgcgcga cggaacgctc tcgagtctgc
      661 gaccggtcga cattctcaac accgggaagc tcggcagcac ctccgtaact ccggcggatc
      721 gcacgatcgc acggttggcc ggcgttagcg gggcggtgcg gccttcgctg tcgtcgccgt
      781 caccgctgat tttggcgacc ttgctggagg ccgcaatcgc cgtcgggcgg ctgtattggc
      841 agagcgcgtc gaatccggcg ttgcagcgcc aggcgatcga gacgggacgt atcgtgtggg
      901 gcaccggcga ggatggacga cagcgtccac ggatcgccgg ccgtccgcac gcggtgctgc
      961 ttgcgtgcaa tcccgtttgg tacgtcgatc ccgtaacgat gcgggccgga ccggtagatc
     1021 ttggtattgc gccggacttg gcagcggcgg cagtatccgc gccggcgctg acggcacggc
     1081 aggcggaacg ttcgcagatt gcctggcgg
//
LOCUS       DASUCJ010000518         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1090563, whole genome shotgun sequence.
ACCESSION   DASUCJ010000518 DASUCJ010000000
VERSION     DASUCJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090563"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFU90_06175"
     CDS             <1..316
                     /locus_tag="VFU90_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479399.1"
                     /translation="GLNSPRGVAVDSKGRIYVTDSNRVLVYAANANGNATPLRTISGS
                     NTQLNGAAGLAVHGSVIVVANSASASITVYPTTGNGNIAPLREISGSLTLLVTPEDID
                     VH"
     gene            480..>1803
                     /locus_tag="VFU90_06180"
     CDS             480..>1803
                     /locus_tag="VFU90_06180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5479400.1"
                     /translation="MKRLTIFALAGALAATAGCGHQGGAPGGFGGGGAGGFSIPVAAA
                     PLIRGDITQTFSVTGTVTPLQTASLSSVVSGTVLAVNAQIGQHVSRGQLLVQIDDSSL
                     QAQLRSSEASLESAQAKLAQTRAGTAGDVASTNAGLLAAQAANQTAQSNLTRDQQLYK
                     EGYVSKQALDQAWSDSMAAAAQLRSAEVAAQNAQVNPSAQSVAQANLKDAQASVDVAQ
                     SQVSLIQSQIAQTQVRAPFDGVVDWRNVDPGSLALPNTTLMQVVQLDPVYIDVGISGS
                     SLQFVHVGTPVEISVGTIPNRTWKGAVQYFNLAAEPGTLTYLARIRVANADLALRGGM
                     VASVAFEQAHKSGVILAPRAAVYETDFGDSMYVIDGGKARVVPVVVGLQNDKLEEVTG
                     AGLAPGVQAILNHPVTLQPGMGVMVMPPGGGPPGAGGGKPGAGKPSSGA"
BASE COUNT          320 a          617 c          598 g          268 t
ORIGIN      
        1 gggcctcaac tcgcctcgcg gcgtcgccgt cgattcgaaa ggccgcatat acgtcaccga
       61 cagcaaccgc gtgctcgtgt acgcggcgaa cgcgaacggc aacgcgacgc cgctccgcac
      121 gatctcgggg tcgaatacgc agctgaacgg cgcggccggc ttggcggtgc acggcagcgt
      181 catcgtcgtc gcgaactcgg cgagcgcgtc aatcacggtc tacccgacga ccggcaacgg
      241 caacatcgcg ccgttgcgcg agatctcagg ctcgctcacg ctgctcgtga cgccggaaga
      301 tatcgacgtc cactaaggcc gcgccggtcg accgtaaagg tcgaccgctc cccgtggatg
      361 gagcgttacc gcgtggtgag acactgcccg ttaggacagt gccatctatg ggcatacccg
      421 aacttaatcc tgacaacgcc gaagggtgcc catatcggcg gcccgtatag ccgttcgata
      481 tgaaacggct tacgattttc gcgctcgcgg gcgcgctcgc cgcaaccgca ggatgcggac
      541 accaaggggg tgctcccggc gggtttggcg gcggcggagc cggcggcttc tcgattcccg
      601 tcgcggcagc gccgctgatc cgcggcgaca tcacccagac gttctccgtg accggcactg
      661 tgacgccgct ccaaaccgct tcgctgtcgt cggtcgtttc cggcaccgtg ctcgcggtca
      721 acgcgcaaat aggccagcac gtctcgcgcg gccagctgct cgtgcagatc gacgacagtt
      781 cgctgcaggc gcagctcagg tcgtccgaag cgtcgctcga atcggcgcag gcgaagctcg
      841 cccagacgcg agccggcacg gcgggcgacg tcgcttcgac gaacgccggg ctgctcgccg
      901 cgcaagctgc gaaccaaacc gcgcagtcga acctaacccg cgatcagcag ctatacaagg
      961 aaggctacgt ctcgaaacag gcgctcgacc aggcgtggtc ggactcgatg gccgcggccg
     1021 cgcagcttcg cagcgccgaa gtggccgcgc agaacgcaca ggtcaacccg tccgcgcaaa
     1081 gcgtcgcaca ggcgaacctc aaggacgcgc aggcatcggt cgacgtcgcg cagtcgcagg
     1141 tctcgctcat ccaatcgcag atcgcgcaga cgcaagtgcg cgcgcccttc gacggcgtcg
     1201 tcgactggcg aaacgtcgat cccgggtcgc tcgcgttgcc aaacacgacg ctcatgcaag
     1261 tcgtgcagct cgatcccgtg tacatcgacg tcggcatctc aggctcgagc ctgcagttcg
     1321 tccacgtcgg cacgccggtc gagatctcgg tcggcacgat cccgaaccgc acctggaagg
     1381 gcgcggtgca gtacttcaac ctcgcggccg agccgggcac gctcacgtac ctcgctcgca
     1441 tacgcgtcgc gaatgccgac ctcgcgctgc gcggcggcat ggtggcgagc gtcgcgttcg
     1501 agcaagcgca caagtcgggc gtcatcctcg cgccgcgggc tgcggtgtac gagaccgatt
     1561 tcggcgattc gatgtacgtc atcgacggcg gcaaggcgag ggtcgtgccc gtcgtcgtcg
     1621 ggctgcagaa cgacaagctg gaagaagtga ccggggccgg gctcgcgccg ggcgtccaag
     1681 cgatcctcaa ccatccggtg acgctgcaac cgggcatggg ggtcatggtg atgcctcctg
     1741 gcggcggacc gccgggagca ggcggcggta agccaggtgc aggcaagccg agctccggcg
     1801 ccg
//
LOCUS       DASUCJ010000519         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1029544, whole genome shotgun sequence.
ACCESSION   DASUCJ010000519 DASUCJ010000000
VERSION     DASUCJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029544"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFU90_06185"
     CDS             complement(<1..879)
                     /locus_tag="VFU90_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5479401.1"
                     /translation="MTGVPSYVQGAASVPLIGETIGAHFDRIAARHADGDALIVRHQN
                     VRWSYRELARRVDDLAVALLGLGLEPGDRIGIWSQNCAEWVLTQFATAKAGLVLVNIN
                     PAYRRVELEYALKKVGCRALVLSPSFKTSDYLAILREVAPEIAASPPGKLHAAALPDL
                     ELVIRLGSAKTPGMLNFDDLATKVTADSLAELARIGDTLQFDDAVNIQFTSGTTGAPK
                     GATLTHHNILNNGFFIGEAMRFTTRDRLCIPVPLYHCFGVVLGSLACVTHGATMVFPG
                     EGFEARAVLETVEAERC"
     gene            1090..>1442
                     /locus_tag="VFU90_06190"
     CDS             1090..>1442
                     /locus_tag="VFU90_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5479402.1"
                     /translation="MAIWKDNTAARPAPAPVPTPVPEAKEPLRFDAAPKPEAPVNQSA
                     VRSEAPLRHDAGHARKESLIAADITIEGKIEGGGSVRIAGRFKGDVNVQGDLTIEAGA
                     KLTGSVRADRVTIAG"
BASE COUNT          230 a          492 c          469 g          251 t
ORIGIN      
        1 gcagcgctcc gcttcgaccg tctccagcac cgccctcgcc tcgaatcctt cgccggggaa
       61 gaccatcgtc gcgccgtggg tcacgcaggc gaggctgccg agcaccacgc cgaagcagtg
      121 gtagagcggc accggaatgc acagccggtc gcgtgtggtg aaacgcatcg cctcgccgat
      181 gaagaagccg ttgttgagga tgttgtggtg cgtcagcgtc gcgcctttcg gcgcgccggt
      241 ggttcccgag gtgaactgga tgttgacggc gtcgtcgaac tgcagcgtgt cgccgattct
      301 cgcgagctcg gcgaggctgt ctgccgtcac cttcgtcgcg agatcgtcga agttgagcat
      361 tcccggcgtc ttggccgatc ccagccggat cacgagctcg aggtcgggca gggccgccgc
      421 gtgcagcttt cccggtggcg acgctgcgat ttccggagcg acctcgcgca aaatcgcgag
      481 atagtcgctg gtcttgaagc tcggcgacag cacgagtgcg cggcagccga ccttcttcag
      541 tgcgtactcg agctcgacgc gccggtacgc ggggttgatg ttgacgagca cgagccccgc
      601 cttcgccgtc gcgaattgcg tcagcaccca ttcggcgcag ttctgcgacc agatgccgat
      661 ccggtcgccc ggctcgaggc cgagaccgag cagcgcgacg gcaagatcgt cgacgcgccg
      721 ggcaagctcg cggtagctcc agcggacgtt ctggtggcgg acgatcagcg cgtcgccatc
      781 cgcatggcgc gcagcgattc gatcgaaatg cgcgccgatc gtctcgccga tgagcggcac
      841 gctcgccgca ccctgcacgt aactcggaac cccggtcatt gcccttcctc cccgcccctc
      901 gttcctcgct tctcgctccc ggattctcct gtttcgattg tcgcacgggg tcccgccaga
      961 gaccgtcgtc ctttgcagga tttactcgca gattcgcctc gttccgacgg tttcttatga
     1021 cttttcggtt gccaccgatc gcgaatggcg ttagcctcac tggccatttg ccgctcggag
     1081 gtcgatacca tggcgatctg gaaggacaac accgcggcgc gccctgcgcc cgcccccgtt
     1141 cccacaccgg tgcccgaggc gaaggagccc ctgcgcttcg acgccgcgcc gaagccggag
     1201 gctcccgtca atcagtcggc ggtccgcagc gaggcgccgc tgcgccacga tgcaggacac
     1261 gcgcgaaagg aatcgctgat cgccgccgac atcacgatcg aaggcaagat cgaagggggc
     1321 ggcagcgtcc gcatcgccgg ccgcttcaag ggggacgtca acgtccaggg cgatctcacg
     1381 atcgaggccg gtgccaagtt gaccggaagc gtccgcgccg atcgcgtgac gatcgcgggc
     1441 ga
//
LOCUS       DASUCJ010000520         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_250041, whole genome shotgun sequence.
ACCESSION   DASUCJ010000520 DASUCJ010000000
VERSION     DASUCJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250041"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFU90_06195"
     CDS             <1..1108
                     /locus_tag="VFU90_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU5479403.1"
                     /translation="MMQSFMSLGWTTVLWFAFGYSESFGPDWHGIIGNPLSYAFLHGV
                     TLHTMFTGNDAGIPLVVHIAYQMMFAIITPALITGAFANRVTFKAYFWFLTGWLIFVY
                     FPFVHMLWSPDGILAKWGVLDFAGGIVVHASAGFAALASVLYVGRRHVIDNKPHNVPL
                     IALGTGLLWFGWYGFNAGSELRVDAVTASAFLNTDVAASFAAIAWLFAEWIRGRQPKF
                     VGLLTGAVAGLATITPAAGYVSPATAVIIGIAAGLVCYAAVTLKNQLGWDDALDVWGV
                     HGVGGFLGIVLLGVFASTLWNPNGADGLLRGNPAFLGKQFVAALVCSAWAFGFTYAML
                     ALINIVTRVKVDEVAEERGLDVELHGEEAYPLGV"
     gene            complement(1159..>1587)
                     /locus_tag="VFU90_06200"
     CDS             complement(1159..>1587)
                     /locus_tag="VFU90_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479404.1"
                     /translation="TYCAQDKALYVADTDNNEIRKVTLEGVVTTIAGSLEAGYADGPA
                     ATARFNHPAGIVCDNVGNLYIADTNNNLIRELATVGTVSTLAGTTDAGTVDGVGAAAR
                     FSHPGDITFDPVTGALYVVDFDSNDIRKVTTSSQGVTSKP"
BASE COUNT          257 a          487 c          487 g          356 t
ORIGIN      
        1 catgatgcag agcttcatgt cgctcggctg gacgaccgtc ctatggttcg cgttcggata
       61 ctcggagagt tttgggcccg actggcacgg catcatcggc aacccgctga gctatgcgtt
      121 cctgcacggc gtgacgctgc atacgatgtt caccggcaac gacgccggca ttccgctggt
      181 cgtccacatc gcctaccaaa tgatgttcgc gatcatcacg ccggcgctga tcacgggagc
      241 tttcgctaac cgcgttacct tcaaagcgta cttctggttc ttgacggggt ggctgatttt
      301 cgtctacttc ccgttcgttc acatgctgtg gagtccggac ggcattctgg cgaagtgggg
      361 cgtactcgat ttcgccggcg gaatcgtcgt gcacgcttcg gccggcttcg cagcgctggc
      421 atcggtgctc tacgtagggc ggcggcacgt tatcgacaac aagccgcaca acgtcccgct
      481 gatcgctctc ggcaccggac tattgtggtt cggctggtac ggtttcaacg ccggctcgga
      541 gctgcgcgtc gatgcggtca ccgcttcggc gtttctcaat accgacgtag ctgcgtcgtt
      601 cgcggccatc gcgtggctct ttgccgaatg gatccgcgga cggcagccaa agttcgtcgg
      661 cttgcttacc ggcgccgtcg ccgggctcgc aacgatcacg cccgcggccg gttacgtgtc
      721 gcccgcaacc gccgtgatca tcggcatcgc cgccggactg gtctgctacg ccgccgtcac
      781 gctcaaaaac cagctcggct gggacgacgc cctcgacgtg tggggcgtgc acggagtcgg
      841 cggcttcctc ggcatcgtgc tgctcggcgt ctttgcatcg actctctgga atcccaacgg
      901 cgccgacggt ctgctgcgcg gcaatccggc gtttctcggc aagcagttcg tcgcggccct
      961 ggtctgcagc gcgtgggcct tcggtttcac ctatgcaatg ctcgcgttga tcaatatcgt
     1021 cacgcgcgtg aaggtggatg aagtagccga ggagcgcggt ctcgacgtcg agcttcacgg
     1081 cgaggaagcc tacccgctcg gggtctgacg gctgtcggcc ttaccacgac ctgtagctta
     1141 cttgggtgag tctagccgct acggtttcga cgtaacgcct tggctggaag ttgttacctt
     1201 ccggatatcg ttcgaatcaa aatcgacgac gtacaatgcg cccgtaacgg ggtcgaacgt
     1261 gatgtcgccg ggatgagaga accgcgccgc cgcgccgacg ccgtcaaccg ttcccgcatc
     1321 cgtagtgccg gcaagggtgc tcacagttcc tacagtcgcg agttcgcgaa tgaggttgtt
     1381 gttagtgtcg gctatataga gatttccaac gttgtcgcag acgattcccg ccggatgatt
     1441 gaatctagcg gttgcggcag gtccgtccgc gtatccggct tcgagcgagc ccgcgatcgt
     1501 ggtcacgacg ccctctaacg tgacctttcg aatttcgttg ttgtcggtgt cggcaacgta
     1561 cagcgcctta tcttgagcgc aataagt
//
LOCUS       DASUCJ010000521         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_739239, whole genome shotgun sequence.
ACCESSION   DASUCJ010000521 DASUCJ010000000
VERSION     DASUCJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739239"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1382)
                     /locus_tag="VFU90_06205"
     CDS             complement(162..1382)
                     /locus_tag="VFU90_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEU5479405.1"
                     /translation="MTAVRAKLSAMMFLEYFVWGAWFVTMGTYLGTSLKFDGTQVGLA
                     YGATAIAAMISPFFVGMVADRFFATEKILALLHIVGGIVLWIASTMHGFGAFYAVLIV
                     YALCYMPTLALTNSISFDHMTDPRREFPLVRVLGTIGWIVAGLIVGVLKLEATAVPLQ
                     LAAAASLVLGVFSFALPHTPPHAAGKTITVRDVLGLDALSLLKDHAFAIFILGSFLLC
                     IPLQFYYTFTNPFLNEIGLQDAAAKMTLGQFFEIGFMLLLPWALSRLGVKRILLLGMA
                     AWAVRYLFFAFGNVTVNVWMLYAGIILHGVCYDFFFVTGQIYVDQRAGVSIRAAAQGF
                     LAFITQGVGLFIGSLVSGPVVDRYRLPGGAHDWRSVWLVPACGAAVIFVLFALLFRPR
                     AQTEEPRPAAAMAA"
     gene            1491..>1725
                     /locus_tag="VFU90_06210"
     CDS             1491..>1725
                     /locus_tag="VFU90_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479406.1"
                     /translation="MTTRLYYSDSYLTEFDARVVDRSEDGRRVYLERTAFYPTSGGQP
                     NDTGTLGGAAVRDVIDEGERIAHVLDTAVTEDAV"
BASE COUNT          356 a          573 c          574 g          222 t
ORIGIN      
        1 cgcccacgcc gatgcaggcg atgttgagcg tatcgctggg cgcgcgatag ccgcggccga
       61 gcacgtgacg cggcacgatg gtgaacgccg cgccggcgag cgcgacgtcg cccagaaact
      121 cccggcggga taccggaccg atggtcatgg aatgcgccgc gttaggcagc catggctgcc
      181 gccggtcgtg gctcttccgt ctgggcacgc ggacggaaga gcagtgcaaa cagcacgaag
      241 atcacggccg cgccgcacgc cggcacgagc cacacggatc gccaatcgtg cgcgccgccc
      301 gggagacggt acctgtccac cacgggcccc gacaccagcg acccgatgaa caggccgacg
      361 ccctgtgtga tgaaggcgag gaagccctgc gccgccgcgc ggatcgacac gccggcgcgc
      421 tgatcgacgt agatctgccc cgtcacgaag aagaaatcgt agcagacgcc gtggaggatg
      481 atgccggcat agagcatcca cacgttcacc gtgacgttgc cgaacgcgaa gaacagatag
      541 cgcaccgccc acgccgccat gcccaacaac agaatccgct tcacgcctaa ccgcgacagc
      601 gcccacggca ggagcagcat gaacccgatc tcgaagaact ggcccaacgt catcttggcc
      661 gcggcgtcct ggagtccgat ttcgttcagg aacgggttgg tgaacgtgta gtagaactgc
      721 agcgggatgc agagcaggaa cgagccgagg atgaaaatcg cgaacgcgtg gtccttgagc
      781 agcgacagcg catcgagccc gagcacgtcg cgcacggtga tcgtcttgcc cgccgcgtgg
      841 ggcggcgtgt gcggcagcgc gaacgagaat acgcctaaca cgagcgaggc cgccgccgcc
      901 agctgcagcg gcaccgccgt cgcctcgagc ttgagaacgc cgacgatgag cccggccacg
      961 atccacccga tcgtgccgag cacgcgcacg agcggaaact cgcgcctcgg gtcggtcatg
     1021 tggtcgaagg aaatcgaatt ggtgagcgca agcgtcggca tgtagcacag cgcgtacacg
     1081 atgagcacgg cgtagaacgc gccgaacccg tgcatcgtgg atgcgatcca caagacgatg
     1141 ccgcccacga tgtggagcag cgccaggatt ttctcggtgg cgaagaaccg gtcggcgacc
     1201 atgccgacga agaacggcga gatcatcgcc gcgatggccg tggcgccgta cgcgaggccg
     1261 acctgcgtgc cgtcgaactt gagcgacgtc cccaggtacg tgcccatggt gacgaaccac
     1321 gcgccccaga cgaagtactc gaggaacatc atcgccgaga gcttggcgcg aaccgcggtc
     1381 atgtccggtc tccagataat ggacggcgcg cgagaagata ccgcgcgcgc cgcacgcagc
     1441 aaggtgaggc gcctctaccg cacgtcgggt ctcgcgcgca tggttcgcgt atgacgacac
     1501 ggctgtacta ctccgactcc tatctcaccg aattcgacgc gcgcgtcgtg gaccgttccg
     1561 aggacgggcg ccgcgtgtac ctcgagcgga cggccttcta cccgacgtcc ggcggccaac
     1621 caaacgacac cggcaccctg ggcggcgcag ccgtgcgcga cgtgatcgac gagggcgagc
     1681 gcatcgcgca cgtgctcgac acggccgtga ccgaagacgc ggtga
//
LOCUS       DASUCJ010000522         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_586419, whole genome shotgun sequence.
ACCESSION   DASUCJ010000522 DASUCJ010000000
VERSION     DASUCJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586419"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFU90_06215"
     CDS             <1..429
                     /locus_tag="VFU90_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5479407.1"
                     /translation="YWMLDNGFLERTHYGDLRDLILRPYSQRDTVVVGPAEPLTTAYQ
                     RMKLYDVSQLPVMDGDKIVGIVDESDVLLHVYGDESKFRDPVSTAMASRLQILDVKSP
                     IESLLKIFEAGRVAIVMDGERFLGLITRIDLLNYLRRRVQ"
     gene            430..>1089
                     /locus_tag="VFU90_06220"
     CDS             430..>1089
                     /locus_tag="VFU90_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5479408.1"
                     /translation="MAKNEKTVGLGTRAIHAGQSPDPSTGAIMTPIYATSTYVQASPG
                     VHKGYEYSRTQNPTRMAYERCVADLEGGKAGFAFASGLAAAATVLDLLDSGSHVIAMD
                     DLYGGTYRLFERVRRRSAGLDFTFIDLNDAAALKAALKPNTRMIWAETPTNPMLKLVD
                     LAKVGAFARKHGLILVVDNTFSSPMLQRPLEHGAHLVLHSATKYLNGHSDMVGGIVVA
                     GD"
BASE COUNT          207 a          360 c          328 g          194 t
ORIGIN      
        1 tactggatgc tcgacaacgg cttcctcgaa cgcacgcact acggcgacct gcgcgatttg
       61 atcctgcgtc cgtattcgca gcgcgacacg gtcgtggtcg gacctgccga accgttgacc
      121 acggcctacc agcgcatgaa actctacgac gtgtcgcaat tgccggtcat ggacggcgac
      181 aagatcgtcg gcatcgtcga cgaatccgac gtgctgctgc acgtctacgg cgacgaatcc
      241 aaattccgcg acccggtatc caccgcgatg gccagccgcc tgcagatact cgacgtgaaa
      301 tcgccgatcg aatcgctgct gaaaatcttc gaggccgggc gcgtcgcgat cgtgatggat
      361 ggggagaggt tcctcggatt gatcacgcgc atcgatcttc tgaattatct acgcaggcga
      421 gtgcagtaaa tggcaaagaa cgaaaaaacc gtgggtctcg gtacccgcgc catccacgcc
      481 gggcaaagcc ccgatccgtc caccggcgcg atcatgacgc cgatctatgc gacctcgacc
      541 tacgtgcagg ccagtcccgg cgtgcacaag ggttacgagt attcgcgcac gcagaacccc
      601 acgcgcatgg cctacgagcg ctgcgtcgcg gatctggaag gcggcaaggc gggcttcgcg
      661 ttcgcctccg gcctggcggc ggcggcgacc gtgctggact tgctcgattc cggtagccac
      721 gtcatcgcga tggacgatct ttacggcggc acctaccgcc tgttcgagcg cgtgcgccgg
      781 cgcagcgcgg gcctggattt caccttcatc gatctcaacg atgcggcggc gctgaaggcc
      841 gcgctcaagc ccaacacacg catgatctgg gccgagacgc cgaccaatcc catgctcaag
      901 ctcgtcgacc tggccaaggt cggcgcgttc gcgcgcaagc acgggctgat cctcgtcgtc
      961 gacaacacgt tttcgtcgcc catgctgcag cgtccgctcg aacacggcgc gcatctggtc
     1021 ctgcattcgg cgaccaagta cctcaatggg cattcggata tggtcggcgg catcgtcgtc
     1081 gccggcgac
//
LOCUS       DASUCJ010000523         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_571275, whole genome shotgun sequence.
ACCESSION   DASUCJ010000523 DASUCJ010000000
VERSION     DASUCJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571275"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFU90_06225"
     CDS             complement(<1..215)
                     /locus_tag="VFU90_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479409.1"
                     /translation="MPNPKPESRYEVLALFAAAMVAASLFVVATGTLTPFLESAFHLG
                     GTQAGLVLSVQMVGSLLATAVAGALTDR"
     gene            275..544
                     /locus_tag="VFU90_06230"
     CDS             275..544
                     /locus_tag="VFU90_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479410.1"
                     /translation="MKRRAELTRYLTIVALAAPAAALVWLVFHRVFASLTQTEAAMGY
                     VDPTTQMGIDLGYLAMFGGTIVFAAIALWAAVCGASLWLRSRRER"
     gene            549..1388
                     /locus_tag="VFU90_06235"
     CDS             549..1388
                     /locus_tag="VFU90_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEU5479411.1"
                     /translation="MRALLVINGRSRAGREAGALAKRELEARGIEVVPFQPRSRGAPP
                     PFDCIVATGGDGTLVPLIGRAMNAGVPIGIVPAGTYNELARTLDLPHGVADACAVIAG
                     GRTRAIDVAQVNGVYYVSEASIGVSSRAARFQTSELKRRYGFWAIVATTLRALWYSRP
                     MFAEVTYDGRVETFKTIQLTVANSHRFGGVFAVADAAIDDGWLDLYSVEIDTFREAFE
                     VTRAILQGKRATVPGLRTLRSTQFSVRQHRRHHVTADGEPAGRTPATFRILPKALRVY
                     VPE"
     gene            1395..2369
                     /locus_tag="VFU90_06240"
     CDS             1395..2369
                     /locus_tag="VFU90_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEU5479412.1"
                     /translation="MRYRTLGHSGLQLSVVGLGSWLTFGSSVDRDATRACVLRAWERG
                     VNFIDTANVYARGAAEQTLGPILQELDRGALVLATKVYFPMGESPNERGLSRKHVRDQ
                     VDLSLARLRVDYIDLYQCHRYDGVTPLEETCGMMNDLVRQGKILYWGVSEWNADQIAA
                     AATLCAARGWPAPIGNQPQYSALWRRVEARVLPTCRRYGLGNVVWSPLAMGILTGKYA
                     QASRPPEGSRAAGPFKDMMEDYLTQPVLDAVQRLVPLAERAGCTLAQLSLAWCLREPA
                     VTSAIVGATKVEHVDENLAAADLDVDPAVFEEMNRILAPVIPSEPYLA"
BASE COUNT          406 a          825 c          823 g          409 t
ORIGIN      
        1 cgatcggtca gcgctccggc caccgcggtc gcgagcagcg agccgaccat ctggaccgac
       61 agcacgaggc cggcctgcgt gccgcccagg tggaacgccg attccaagaa cggcgtcagc
      121 gtcccggtcg cgacgacgaa gagcgaggcc gcaaccatcg ccgcggcaaa gagcgcgagg
      181 acttcgtaac gcgattcggg tttgggattc ggcatcggca aactatacgg tttccccgca
      241 ccgcctcccc gcaagcgcgc cggagcgatg aaaaatgaag cgtcgagcgg aacttactcg
      301 ctatctcacg atcgtcgcgc tggccgcgcc ggcggccgcg ctggtgtggc tggtgttcca
      361 ccgggtgttc gcgagcttga cgcaaaccga ggcagcgatg ggttacgtag atccgacgac
      421 tcagatgggt atcgatctcg gatatctcgc gatgttcggc gggacgatcg tctttgccgc
      481 gatcgcgctg tgggccgccg tttgtggcgc gagcctttgg ctgcgctcgc gccgcgagcg
      541 ctaagcggat gcgcgcgctg ctggtgatca acggccgttc gcgcgccggc cgcgaggcgg
      601 gcgccctcgc gaagcgcgaa ctcgaggcgc gcggtatcga ggtcgttccc tttcaaccgc
      661 gctcccgcgg cgcaccgccg cccttcgact gcatcgtcgc aaccggcggc gacggcaccc
      721 tcgtgccgct gatcgggcgg gccatgaacg ctggggtccc gatcggcatc gtgccggccg
      781 gaacgtacaa cgagctggcg cggacgctcg accttccgca cggcgtcgcc gatgcttgcg
      841 cggtcatcgc cggcggacga acgcgtgcca tcgacgtcgc gcaagtcaac ggcgtctact
      901 acgtcagcga agcgagcatc ggcgtctcca gccgggcggc gcgcttccaa accagcgagc
      961 tcaagcggcg ctacggattc tgggcgatcg tcgctacgac gctgcgcgcg ctatggtatt
     1021 cgcggccgat gttcgccgaa gtcacctacg acggccgcgt cgaaacgttc aaaacgattc
     1081 agctgaccgt tgccaatagt caccgcttcg gcggagtgtt cgcggtcgcc gacgcggcca
     1141 tcgacgacgg atggctcgac ctctattccg tggagatcga caccttccgg gaagcttttg
     1201 aggtaacgcg cgccattctc cagggaaagc gcgcaaccgt tccgggtctg cgaacgctgc
     1261 gttcgacgca gttctccgtc cgtcagcatc gccggcatca cgtgaccgcc gatggcgagc
     1321 cggccggacg aacgcccgcg acctttcgca ttctgccgaa ggctttgcgc gtctacgtgc
     1381 ccgaatgacc gcccatgcgc tatcgaacgc tcgggcattc cggtctgcag ctgtcggtcg
     1441 tcggcctcgg gtcgtggctg acgttcggca gcagcgtcga tcgcgacgcg acccgcgcct
     1501 gcgtcctgcg tgcttgggag cgcggcgtga acttcatcga cacggcgaac gtgtacgcgc
     1561 gaggtgcggc tgagcagacg ctcgggccga ttctccagga gctcgaccgc ggcgcgctcg
     1621 tgctcgccac caaagtgtat tttccgatgg gcgagtcgcc caacgaacgc gggctttcgc
     1681 gcaagcacgt tcgcgatcaa gtcgacctgt cgttggcgcg cctgcgcgtc gattacatcg
     1741 acctctacca atgtcaccgc tacgacggcg tcacgccgct cgaagaaacg tgcggcatga
     1801 tgaacgacct ggtgcggcag ggaaagattc tctactgggg cgtttcggag tggaatgccg
     1861 atcagatcgc tgcggccgca acgctctgtg ccgctcgcgg ctggccggcg ccgatcggca
     1921 accagccgca gtacagcgcg ctctggcggc gcgtcgaagc gcgcgtcctg ccgacgtgcc
     1981 gccgctacgg tctcggaaac gtcgtgtggt cgccgctcgc gatgggaatt ctcaccggga
     2041 aatacgcgca ggcatcgcgt ccgcccgagg ggagccgggc ggccgggccc ttcaaggaca
     2101 tgatggaaga ctacctcact cagcccgttc tcgacgcggt gcaacggctc gttccgcttg
     2161 ccgaacgggc gggatgcacc ctcgcccagc tgtcgctggc gtggtgcctg cgcgagcccg
     2221 ccgttaccag cgcgatcgtg ggcgcgacca aggtcgagca cgtcgacgag aatctggccg
     2281 ccgccgacct ggacgtcgac ccggccgtct tcgaggaaat gaaccggatt ctggcgccgg
     2341 tgatcccgtc cgagccgtat ctcgcctagg ggaaaaagca aagcaagggg gcggccgccg
     2401 ggcggagccc ttcaacttgc ccccgccgga tgaaaacttt gcccggactg cggcgtataa
     2461 gag
//
LOCUS       DASUCJ010000524         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1426859, whole genome shotgun sequence.
ACCESSION   DASUCJ010000524 DASUCJ010000000
VERSION     DASUCJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426859"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1817)
                     /locus_tag="VFU90_06245"
     CDS             complement(219..1817)
                     /locus_tag="VFU90_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479413.1"
                     /translation="MRTRLLLLSTALSFLAVGTTAAQQRQGPPGQRPGAQRPAEQRDS
                     GAADSTAGVAPVERVSTTQHTITVDGKPVAYTARAGTMVLRDSTGKPAATVFYISYTK
                     DGVDAANRPVTFFFNGGPGSASIWLDEGIMSPKHPEMGPAGSEPAPPYNLVDNPNSPL
                     DVTDLVQVDAMMTGYSRPAPGVKTSEFTGAVNDVAMFGQFIRDYLDKYDRWASPKYLF
                     GESYGTFRSAGLASELQEREGIELNGIMLLGTVLDFQYISQEPTNDIAYSTFLPTYTA
                     TAWYHKKLPSSLQSESLEQVVAQSRQYAFGDYMTALAKGNDLSQSERQAVAQKLAQLT
                     GISEQYLMNTNLRLDAGSFRTELLRDERLIMGRYDTRLTGINGNAANQRQDYDPSDAA
                     PSGAFMAAFMRYLHQDLKYTTDLQYYMGGHAGRWDYSSLNSRGFPSEVESLRLAMAKD
                     PYLHVMVGAGYYDTATPFANAEYTFSHLGFDQTYKDRVQFKYYESGHMAYLNQASAKQ
                     LKSDIANFIASTQHLTPVTAQATH"
     gene            2392..4461
                     /locus_tag="VFU90_06250"
     CDS             2392..4461
                     /locus_tag="VFU90_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HEU5479414.1"
                     /translation="MNALAVDPDRVPPDPAPSPAVTRRDFLERSGGLVLGIGALSLGI
                     APAGVAPSRRSDEIVLGNDAITAAWTTAGGALRPLRLTDGVNTTALPVPAQVFTLAFA
                     DKTTLDASDMRIVAAPVTETLAPNAAAPRGVERVGGRRVTVRLRDTAGRIEADWRAVL
                     RDGGAYVRQELVLRALGADVPLRSVTMFAVRAPNAMVPGTVRGTPVVIGNAYVAVEHP
                     LADNVVDGDDVRCRATRTLPLRRGAALELSSVIGVTRPGQLRRDFLAYVERERAHPYR
                     TFLHYNSWYDIGYFNRYSEADALGAITALGRELHERRGVRLDSFLFDDGWDDPATLWH
                     FNAGFPDGFTKVAAATHRFGAAPGVWLSPWGGYGKPREERLAYGKQQGFETNADGFAL
                     SGPIYYKRFRDTCVDMIRRYGVNQFKIDGTGDASSASPGSPFDSDFDAAIHLIGELRA
                     EEPDLYVNLTTGTYPSPFWLRYADSIWRGGDDHDFTGVGSSRQRWITYRDADTYDHVV
                     RAGALFPLNSLMLHGMIYAKQAHNLMTDPGGDFPSEVRSYFGTGTQLQEMYVTPALLS
                     SRDWDTIAECATWSRTNAATLRDTHWVGGDPQRLEPYGWASWSPEKGILTLRNPSNAT
                     QEIALDVARAFELPDGAPRRYAAVSPWASHRDAGALDLAAGAEHRFSLAPFEVVTLEC
                     TPTRATR"
     gene            4575..>5596
                     /locus_tag="VFU90_06255"
     CDS             4575..>5596
                     /locus_tag="VFU90_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479415.1"
                     /translation="MRLTPLDWVIVAVSLALCYLPALFYIRRSQSGLAEFFASGQSAP
                     WWLVGTSMVATTFSTDTPNLVTDFVRSHGVSYNWVWWAFLLTGMATVFFYAQLWRRSG
                     VLTDLEFYELRYAGKPAALVRGFRAVYLGFFFNIMIMSTVTLAAIKIANVMLGWDRLE
                     TVVIAGTACVLFASISGLWGVLTTDLVQFALAMIGVIAAAVVALQQPQVGGLTGMLSK
                     VSPRTLNLLPDFHDTSLTLSVLIIPLTIQWWSVWYPGSEPGGGSYIAQRILASRNERH
                     ALGATLWFNVANYALRPWPWIIVALCSMLVFPTLGDIHRAMPHVDPALIGNDLAYPAM
                     LTLLPVG"
BASE COUNT          863 a         1824 c         1907 g         1002 t
ORIGIN      
        1 cacagcaagc tcgagttcaa ctcccggcgg ggtctcgaga aaaccaccga gacgctggtg
       61 catctggtcc agctcgtcgc ggaacgcgag tcctgacggg cggcacgcgc gccgctgcgg
      121 cgcgaacccc ctgaacggcg aatcgcccgg acgcatcgtg cgcgtccggg cgattcgccg
      181 tgcggcgatc cgttaggcgg atggcggcgc ggcgccgctc agtgcgtcgc ttgggccgtg
      241 accggcgtca gatgctgcgt cgacgcaatg aaattcgcga tgtccgactt gagctgcttg
      301 gcggacgcct gattcaggta cgccatgtgg ccgctctcgt agtacttgaa ctgcacgcga
      361 tccttgtacg tctggtcgaa ccccagatgc gagaacgtat actcggcgtt cgcgaacggc
      421 gtcgcggtgt cgtagtagcc cgcgccgacc atgacgtgca ggtacggatc tttcgccatc
      481 gccagacgca gcgactcgac ttcgctcggg aagccgcgcg aattgagcga gctgtagtcc
      541 cagcggccgg cgtggccgcc catgtaatac tgcaggtcgg tggtgtactt gagatcctgg
      601 tgcaggtatc gcatgaacgc cgccatgaac gcgcccgacg gcgcggcgtc ggatggatcg
      661 tagtcctgcc gctggttggc cgcgttgccg ttgatgcccg ttaggcgcgt gtcgtaccgg
      721 cccatgatca gacgctcgtc gcgcagcaac tcggtgcgga agctgccggc gtcgaggcga
      781 aggttggtgt tcatcaagta ctgctcggag atgccggtga gttgcgccag cttctgcgcc
      841 accgcctgcc gctcggattg cgagagatcg ttgcccttcg ccaatgcggt catgtagtcg
      901 ccgaacgcat actggcgcga ctgcgccacc acctgctcga gcgactcgct ctgcaggctc
      961 gacggcagct tcttgtgata ccatgccgtg gccgtgtacg tcggcaggaa cgtcgagtac
     1021 gcgatgtcgt tggtcggttc ctgtgagatg tactggaagt cgagcaccgt gcccaacagc
     1081 atgatcccgt tgagctcgat gccttcccgc tcctgcagct cgctcgccag accggccgag
     1141 cggaacgtgc cgtagctctc cccaaagagg tatttgggcg atgcccaacg atcgtacttg
     1201 tcgaggtagt cgcggatgaa ctgcccgaac atcgcgacgt cgttcaccgc gccggtgaac
     1261 tcggaggttt tgacgccggg cgcggggcgc gagtagccgg tcatcatcgc gtccacctgc
     1321 accaggtcgg tcacgtctaa cggagaattc ggattgtcga ccaggttgta cggcggcgcg
     1381 ggttcagacc ccgcgggccc catctccgga tgcttcgggc tcatgatgcc ctcgtccagc
     1441 cagatcgacg ccgagccagg accgccgttg aagaagaacg tcaccgggcg gttggcggca
     1501 tcgacgccat ccttcgtgta cgagatgtag aacacggtcg cggcgggttt ccccgtggag
     1561 tctcgcagca ccatcgtgcc ggcgcgcgcc gtataggcga cgggttttcc gtcgacagtg
     1621 atcgtgtgct gtgtggtcga cacacgctcg accggcgcga cgccggccgt ggagtcggcc
     1681 gcaccgctgt ctcgttgctc ggcgggacgt tgagcgcccg ggcgctgtcc gggcggtcct
     1741 tgccgttgtt gggccgcggt cgtgccgacg gccaggaatg agagagcggt gctgagcagc
     1801 agcaaacgcg tgcgcatgac gggcactcct tgagaccggt tcacagtggg atcgaacgac
     1861 ggtggtgatc gagcatagat gtcgtcacga taggaaaacg aagaggtggc tcggatcgct
     1921 gcacgatccg cgatcgagtg gcgcggctga ttcgaatgaa tgggaggcga ctggctgcgg
     1981 aggcgaatcg ccgcgcgcgg ccgccagggc taaagtgttg taacgcaaga gattgcggca
     2041 gccactcgcc gtcagtgata cggcctcgag ggcaatcgag cgattccagc cgcgcatgtc
     2101 gaggattgtg tcgcccgtgc cgtcgatcgt aaaggaccct cgagacagcg gggggattgg
     2161 cggcgaccgt cgttctcgtg acgttcgaca gcgcatcgga cggaacaccg aacctgtccc
     2221 gggcggcgag ttgcgcgccg cctaacgaac cggctggacg gtgtcctcgc cgtcgtccgc
     2281 gagcagagac gaagttgtcg agccggctgc cggcgccgca tgctttcctg cgccaggctc
     2341 cattgacgat gccgctgcgg cgcgatagtc tcgccacggc gcatccatcg agtgaacgca
     2401 ttggccgtcg accccgaccg tgtaccgccg gatcctgctc catctcccgc cgtcacgcgc
     2461 cgtgactttc tcgagcgcag cggcggcctg gtgctcggca tcggcgcgct ctcgttaggc
     2521 atcgcgccgg cgggcgttgc cccgtcgcgc cgctcggacg agatcgtgct gggcaacgac
     2581 gccatcaccg ccgcgtggac cacggccggc ggggcgctgc ggccgctgcg tctcaccgac
     2641 ggtgtgaaca cgaccgcgct gccggttccg gcgcaggtct tcacgctcgc cttcgccgac
     2701 aagaccaccc tcgatgcgag cgacatgcgc atcgtggccg cgcccgtcac cgagacgctc
     2761 gcgccgaacg cagccgcgcc gcggggcgtc gagcgcgtgg gcggacgccg cgtgacggtg
     2821 cgcctgcgcg ataccgccgg ccggatcgaa gccgactggc gcgcggtgct tcgcgacggc
     2881 ggcgcctacg tgcggcagga actggtgctc cgcgcactgg gcgccgatgt gccgctgcgc
     2941 tcggtgacga tgttcgccgt ccgcgcgcct aacgcgatgg tgccggggac ggtgcgcggc
     3001 acgccggtgg tgatcggcaa cgcgtacgtg gcggtcgaac atccgctcgc cgacaacgtc
     3061 gtcgatggcg acgacgtgcg ctgccgcgcc acacgcacgc tgccgctgcg tcgcggcgcc
     3121 gcgctcgagc tcagcagcgt gatcggcgtg acgcggcccg gccagctgcg gcgcgacttc
     3181 ctggcgtacg tcgagcgcga gcgggcgcac ccgtatcgga cgttcctgca ctacaactcg
     3241 tggtacgaca tcgggtactt caaccgctat agcgaagccg atgcgctggg cgccatcact
     3301 gcgttaggca gagagttgca cgagcggcgc ggcgtcaggc tcgacagctt cctgttcgac
     3361 gatggatggg acgaccccgc cacgttgtgg cacttcaacg ccgggttccc ggacggcttc
     3421 acgaaggttg ccgccgccac acaccgcttc ggggccgcgc cgggcgtgtg gctctcgccg
     3481 tggggcggat acgggaaacc gcgtgaggaa cggcttgcct acgggaagca gcaggggttc
     3541 gagacgaatg ccgatggctt tgcgttgtcc ggtcccatct attacaagcg cttccgcgac
     3601 acgtgcgtcg acatgattcg ccggtacggc gtcaaccaat tcaagatcga cggcaccggc
     3661 gatgcgtcga gcgcgagtcc cggcagtccg ttcgacagcg acttcgacgc cgcgattcat
     3721 ctcatcggcg agctgcgcgc ggaagaaccc gacctctacg tgaacctcac cacgggaacg
     3781 tatccatcgc cgttctggct gcgatacgcc gattcgatct ggcgcggcgg cgacgatcac
     3841 gatttcaccg gcgtcggctc gtcgcgccaa cgatggatca cctatcgcga cgcggatacc
     3901 tacgaccacg tggtgcgcgc cggcgcgctc ttccccttga actcgctcat gttgcacggg
     3961 atgatctacg cgaagcaggc gcacaacctg atgacggatc ccggcggcga tttcccgagt
     4021 gaagtgcgga gctatttcgg aaccggcacc cagttgcagg aaatgtacgt cacacccgcg
     4081 ctcctctcat ctcgtgactg ggacacgatc gccgagtgcg cgacgtggtc gcgaacgaac
     4141 gcggcgacgc tgcgcgacac gcactgggtg ggcggcgatc cgcagcggct cgagccgtac
     4201 ggttgggcgt cgtggtcgcc ggagaagggc attctcacgt tgcgcaaccc gtccaacgcg
     4261 acgcaagaga tcgcgctcga tgtcgcgcgc gcgttcgagt tgccggacgg cgcaccgcgg
     4321 cggtatgcgg ccgttagtcc gtgggcatcg catcgcgacg cgggcgccct ggatctcgcg
     4381 gcgggggcgg agcaccgctt ctcgctcgcg ccgttcgagg tcgtgacgct cgagtgcacg
     4441 ccgacgcgag ccacgcggtg accgggacgc tcgcgcagcc gccgatcggt ccagcatgat
     4501 tccccgcgcc ggtcattcac gtcgcggacg cgggtgccgc gggcacgcgc gcgtgcaccg
     4561 gttgatcgcc tgacgtgagg ctcaccccgc tcgactgggt catcgtcgcc gtcagtctgg
     4621 cgctctgcta cctgccggcg ttgttctaca ttcggcggtc gcaatccggc ctggcggagt
     4681 tcttcgcgtc ggggcaatcg gcgccgtggt ggctcgtggg cacgtcgatg gtcgcgacca
     4741 cgttcagcac cgacacgccg aacttggtca ccgacttcgt gcggtcgcac ggggtgtcgt
     4801 acaactgggt ctggtgggcg ttcctgttga ccggcatggc caccgtcttt ttctatgccc
     4861 aactgtggcg acggtcgggc gtgctcacgg atctcgagtt ctacgagctg cgctacgcgg
     4921 ggaagccggc ggcgctcgtg cgcggattcc gcgcggtgta cctgggcttc ttcttcaaca
     4981 tcatgatcat gtcgaccgtg acgctcgccg cgatcaagat cgccaacgtg atgttgggct
     5041 gggaccgact cgagaccgtc gtgatcgccg gcacggcgtg cgtgctcttc gcgtcgatct
     5101 cgggtttgtg gggcgtgctc accaccgatc tcgtgcagtt cgcgctggcg atgatcggcg
     5161 tgatcgccgc ggcggtcgtg gcgctccagc agccgcaggt tggcggccta acgggaatgc
     5221 tgtccaaagt gagcccgcgc acgctcaacc tgctgccgga ttttcacgat acgtcgctca
     5281 cgctcagcgt cctcatcatt ccgctcacca tccagtggtg gtcggtgtgg tatccgggat
     5341 cggagccggg cggcgggagt tacatcgccc aacgcattct ggcgtcgcgc aacgagcgcc
     5401 atgcgttggg cgcgacgctg tggttcaacg tcgccaatta cgcgctcagg ccgtggccgt
     5461 ggatcatcgt cgcgctgtgc tcgatgctcg tgtttcccac gttgggtgac atccatcgcg
     5521 cgatgccgca tgtggacccg gcgctcatcg gcaacgacct cgcctacccg gccatgctca
     5581 cgctgctgcc ggtggg
//
LOCUS       DASUCJ010000525         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_815805, whole genome shotgun sequence.
ACCESSION   DASUCJ010000525 DASUCJ010000000
VERSION     DASUCJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815805"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1550)
                     /locus_tag="VFU90_06260"
     CDS             complement(39..1550)
                     /locus_tag="VFU90_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5479416.1"
                     /translation="MVLPISAIGAQGTRSDRWRAVARADLDAVHRTIVEAHPGAIDAE
                     NPSFRTWMEDGYRQALALLPHVTDYSTALDAVRWYVTGFRDGHLLLSDDTRPSDELIP
                     SKGWGVAMRHGHFVVTNVASHWQAALPPPGATVLSCDGRSPQAIATEDVAPYIDRRDL
                     PLSRQIVAEYIGEPSLSMRKRLKSCTFAPSDGRAAMALDVVYRPLVFEQWASIMNDYR
                     RPRARVNDYDLTDGLLWIRAQTFSLDHEQYAVLESMLQELRRLHGVRAIVFDVRGNGG
                     GNSEFGGEIFDAATGGLRYDHRGLTRLPAIYAEWRVSAISIEAAKTGLANALRVSGPG
                     SSTVEWYRDHLRSLLVAQAAGKPWARQDTGERRLTRKEMKRRQARLRRFAGPIAVLTD
                     EYCASACLDFVDLVRQVPGALQLGATTGADSVYIDTGRVRLPSGNHLVLPLKVWRNRL
                     RSNNEAMVPDITYDGDLDDDAAVRIWVVSVLAGRGSRNAFQAATHHAAVIARP"
BASE COUNT          325 a          590 c          576 g          288 t
ORIGIN      
        1 ggacgcagcg atgcgctcat cgggcccgac cagcgacgtc acgggcgagc gatgacggcc
       61 gcgtggtgcg tcgcggcctg aaatgcgttc cgggaaccgc ggcctgcaag taccgaaacg
      121 acccatatgc gaaccgcggc atcatcgtca agatcgccgt cgtacgtaat gtcggggacc
      181 atcgcctcgt tgttgctccg cagtcggttg cgccacactt tcaacggcag aaccaggtgg
      241 ttgccgctgg gcaggcgtac ccttcccgtg tcgatgtaga cggagtcagc gccagtcgtc
      301 gcgccgagct gcagagcgcc cggtacctga cgcacgagat ccacgaaatc caggcacgcg
      361 ctcgcgcagt actcgtcggt cagcaccgcg atcggccccg cgaagcgccg caatcgggcc
      421 tgccttcgct tcatctcctt gcgagtaagc cgtcgctcgc ccgtgtcctg gcgcgcccag
      481 ggtttcccgg ccgcctgggc gacgagcagt gaccgcaagt ggtcgcgata ccactcgacc
      541 gtgctgctgc caggaccgga cacccgcagt gcgttcgcga gtcccgtctt cgcggcctcg
      601 atcgatatcg ccgacacgcg ccactccgca tagatggcgg gcaacctggt gaggccgcgg
      661 tggtcgtagc gaagaccacc cgtcgcggcg tcgaagatct ctccgccgaa ttcgctgttg
      721 ccgccgccgt tgccgcgcac gtcgaagacg atcgcgcgaa cgccatgcag gcggcggagc
      781 tcctgcagca tcgattcgag caccgcgtac tgctcgtgat cgaggctgaa ggtctgcgcg
      841 cggatccaca gcagcccgtc cgtcagatcg tagtcgttga cgcgagcgcg cgggcgacga
      901 taatcgttca tgatcgatgc ccactgctcg aacaccagcg gtcgatagac gacgtcgagc
      961 gccatcgcgg cgcgaccgtc ggagggcgcg aacgtacacg acttcagccg cttccgcatc
     1021 gaaaggcttg gttcgccaat atattcggcg acgatctgcc ggctcaacgg caggtcccgt
     1081 cgatcgatgt acggagcaac atcctccgtg gcgatcgcct gcggtgagcg tccatcgcac
     1141 gaaagcacgg tcgcgcccgg tggcggtagc gcggcctgcc agtgcgaagc gacgttcgtg
     1201 acgacgaagt ggccgtggcg catcgccacg ccccaaccct tggaaggaat cagctcgtcc
     1261 gacgggcgcg tatcgtcgga gagaagcaga tggccatcgc gaaagccggt aacgtaccag
     1321 cggacggcat cgagcgccgt gctgtaatcg gtgacgtgcg gcaatagtgc cagagcctgc
     1381 cggtagccat cctccatcca ggtccggaac gacggattct ctgcgtcgat cgcgccgggg
     1441 tgcgcctcga cgatcgtcct gtggacggca tcgaggtcgg cccgcgcgac ggcgcgccaa
     1501 cggtccgagc gcgtgccttg cgcaccgatt gcagaaatgg gcagcaccat tgcgagaccg
     1561 aggagcgacc aaacgaatcg attgcgtgcg aacgtctggg ttgcattcaa aggcattgcc
     1621 atcccctggg aagtggcgga tgtgcgtatc ctgcactagc ggaccgcgta gatgggcatc
     1681 gccgcgccgg gtgatccgac cgaggcgaga ctgaccaccg attcaccatc cgttccaagt
     1741 tccgcactcg cgggcgtcac aagtcgcaac cgacggcaa
//
LOCUS       DASUCJ010000526         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1457519, whole genome shotgun sequence.
ACCESSION   DASUCJ010000526 DASUCJ010000000
VERSION     DASUCJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457519"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2274)
                     /gene="ppdK"
                     /locus_tag="VFU90_06265"
     CDS             complement(<1..>2274)
                     /gene="ppdK"
                     /locus_tag="VFU90_06265"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU5479417.1"
                     /translation="LGLNDATVAALARLTGNERFAWDSYRRFVAMFAHVVLGVDKDRF
                     EELIGEAKAALGVRKDPEVDAATWQRLVDRFKRVVREETGLDFPQDVREQLRLAIAAV
                     FDSWNCKRAKDYRRFNKIPDDWGTAVTVMEMVFGNTGDDSGTGVAFTRDPNTGEKRLF
                     GEYLRNGQGEDVVAGIRTPEKIADLAQSQPGVFQQFQEIAGRLERHYRDMQDLEFTVE
                     RGKLFMLQTRSGKRSAEAAVKIALDLVNEGLIDRRRAVAMVDAQSLDQLFHARIDPNE
                     RVQVAGKGLNASPGAAAGQIVFSADDAVSWKEAGKHTILVRVETNPDDVHGMIAAEGI
                     LTAKGGATSHAAVVARGMGKPCVAGCDALEIDRRKKVATLGGITFEEGDWITIDGTTG
                     NVAAGRLSLVPPPSALPPWLRTFLEWADELRRMEVWANADTPEDAARARALGAQGIGL
                     CRTEHMFMQQDRLPTVQRMILADDLAAREDALAKLLPFQRDDFAGILAAMEGLPVTIR
                     LLDPPLHEFLPSLEELLVQVTELRIQRGSDSPDFKIKDAVLHRVLQLHEQNPMLGLRV
                     CRLGIVYPEIYAMQVRAIFEAACDLKARGVDARPEVMIPGVGTKEEMQTTRAAVEAVA
                     AEVLAERDVTLEYHVGTMIELPRACVVAGELAEHAEFFSFGTNDLTQTTYGYSRDDAE
                     ASFIPVYLEKRILKSDPFQVLDRQGVGSLMREAVRLGRGARDGLKIGICGEHGGEPSS
                     VAFCESLGLNYVSCSPY"
BASE COUNT          358 a          777 c          729 g          410 t
ORIGIN      
        1 ggtacggcga gcacgaaacg tagttgagcc ccaggctttc gcagaatgcg acgctgctgg
       61 gctcgccgcc gtgttcgccg cagatgccga tcttgagacc gtcgcgcgcg ccgcgcccga
      121 gccggacggc ctcgcgcatc aggctcccga cgccctgccg gtcgagtact tggaagggat
      181 cgctcttaag gatccgcttc tcgaggtaaa ccgggatgaa cgacgcttcg gcgtcgtcgc
      241 ggctgtagcc gtaggtcgtt tgcgtgaggt cgttggtgcc gaacgagaag aactccgcgt
      301 gttcggccag ctcgccggcg acgacgcaag cccgcggcag ctcgatcatc gtgccgacgt
      361 ggtactcgag cgtgacgtcg cgctccgcca atacctcggc cgcaaccgct tcgacggcgg
      421 cccgggtcgt ttgcatttcc tccttggtac cgaccccggg gatcatcact tccggccgcg
      481 catccacccc gcgcgccttc agatcgcacg cggcttcgaa aatcgcccgc acctgcatcg
      541 cgtaaatctc gggatagacg atgccgagcc ggcagacgcg caggccgagc atcggatttt
      601 gctcgtgcag ctgcagcacc cgatggagca cggcgtcttt gatcttgaaa tcgggggaat
      661 cgctgccgcg ctggatacgc agctcggtga cttggaccag cagttcttcg agcgacggca
      721 ggaactcatg cagcggcgga tcgagcagcc ggatcgtcac cggaagccct tccatcgccg
      781 cgaggatgcc ggcgaaatcg tcgcgctgaa acggcagcag cttcgcgagc gcgtcttccc
      841 gcgccgccag gtcgtcggcg agaatcattc gctgcacggt cgggagccgg tcctgctgca
      901 tgaacatatg ctcggttcgg cataacccga tgccctgcgc gccgagcgcg cgggcccgcg
      961 cagcgtcctc cggcgtgtcg gcgttcgccc acacctccat ccggcgcagc tcgtccgccc
     1021 attccaggaa cgtgcgcagc cacggcggca gcgccgacgg cggaggaacc agcgacagcc
     1081 gtccggccgc aacgttgccg gtggtgccgt cgatcgtgat ccaatcgcct tcctcgaacg
     1141 tgatgccgcc gagcgtcgca actttcttgc ggcggtcgat ttcgagcgcg tcgcaaccgg
     1201 cgacgcacgg ctttcccatc ccgcgggcga ccaccgccgc gtgcgaggtg gcaccgccct
     1261 tggcggtgag gatgccttcg gcggcgatca tcccgtgcac gtcgtcggga ttggtctcga
     1321 cgcggacgag tatcgtgtgt ttgccggctt ccttccagct gacggcgtcg tcggccgaga
     1381 agacgatctg cccggcggca gctcccggcg acgcgttgag ccccttgccg gcgacctgca
     1441 cgcgttcgtt cggatcgatt cgcgcgtgga agagctggtc gagcgattgg gcgtcgacca
     1501 tcgcgaccgc gcgccggcga tcgatcaggc cttcgttcac taagtcgagc gcgatcttca
     1561 cggcggcttc ggcgctgcgc ttgccgctgc gcgtctggag catgaagagc ttgccgcgct
     1621 ccacggtaaa ctccaggtcc tgcatgtcgc ggtagtgacg ttcgagccgg ccggcgattt
     1681 cctggaactg ctggaagacg ccgggctggc tctgcgctag gtccgcaatc ttctcgggcg
     1741 tgcgaatgcc ggcgacgacg tcttcgccct gtccgttacg caggtattcg ccgaacagcc
     1801 gcttctcgcc cgtattcgga tcgcgcgtaa aggcgacgcc cgttcccgaa tcgtcgcccg
     1861 tattgccgaa caccatttcc atgacggtga cggcggttcc ccaatcgtcg ggaatcttat
     1921 tgaagcggcg atagtctttc gcgcgcttgc agttccaaga atcgaaaacc gccgcaatcg
     1981 cgagcctgag ctgttcgcgc acgtcctgcg ggaaatcgag cccggtctcc tcgcgcacga
     2041 cccgtttgaa gcggtcgacc agccgctgcc aggtcgccgc atcgacttcc ggatccttgc
     2101 gtaccccgag cgccgccttg gcctcgccga tcagctcttc gaagcgatct ttatcgacgc
     2161 cgagcacgac gtgcgcgaac atcgccacga accgccggta cgagtcccac gcaaaccgct
     2221 cgttgccggt gaggcgcgcc agcgcggcga cggtcgcgtc gttgagcccg aggt
//
LOCUS       DASUCJ010000527         1119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1381081, whole genome shotgun sequence.
ACCESSION   DASUCJ010000527 DASUCJ010000000
VERSION     DASUCJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFU90_06270"
     CDS             complement(<1..541)
                     /locus_tag="VFU90_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479418.1"
                     /translation="MRLTYLFAACALSAPVAAQAPVLRTVADVPVPGHAVRFDYQSLD
                     TAANRLYVAHMNAGQLVVFDVATRRVIATLDGFDRVHGVWAVPELGRVYASVTGRHEV
                     AVVDAKSLAVLARVPGIDYPDGIAYAPAVRRVFVSDERGRADGVIDATTNTLVTSVPL
                     GGEAGNTVYDPTAKRILVAV"
     gene            complement(548..>1119)
                     /locus_tag="VFU90_06275"
     CDS             complement(548..>1119)
                     /locus_tag="VFU90_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479419.1"
                     /translation="SGDDFQVVIIRDLDAPTDTFSLARGVAIQRGRYWWTQAGAQFQS
                     SHGRPISATALFSTGTFYDGHSTETDGGLTWRSSGHLILGAGVSRTDVTLPAAHFVAI
                     QSTGRVEYAFTTRMDFLAFAQYENALHRSDFDLRFHWTPVIGDDLYVVWNSGYTNDPL
                     SPFRFPDSRTIARPLTATFTVKYVHRIAP"
BASE COUNT          205 a          366 c          370 g          178 t
ORIGIN      
        1 gcacagcgac cagaatgcgc ttcgccgttg ggtcgtatac ggtgttgccc gcctcgccgc
       61 cgaggggcac cgacgtgacg agggtgttcg tcgttgcgtc gatcacgccg tctgctcgtc
      121 cgcgctcgtc cgacacgaac acccgcctaa cggccggggc gtacgcgatt ccatctggat
      181 agtcgatgcc cggcacgcgc gcgagcacgg ccagcgattt cgcatcgacg accgcgacct
      241 cgtgcctgcc ggtcaccgac gcgtacacgc gtccgagttc gggcacggcc cacacgccgt
      301 gtacgcgatc gaacccgtcc agggtcgcga tcacgcgacg cgtcgccacg tcgaacacga
      361 cgagctgccc ggcgttcatg tgcgcgacat agagccggtt ggccgccgtg tcgagactct
      421 ggtagtcgaa cctgacggcg tgtccgggca ccggcacgtc ggcgacggtg cggaggaccg
      481 gcgcctgggc ggcgaccggt gcggacaggg cacacgcggc gaaaagatac gttaggcgca
      541 tggcgattca tggtgcgata cggtggacgt acttgaccgt gaaggtcgcg gtcaacggcc
      601 gggcgatcgt ccggctgtcg ggaaatcgaa acggcgataa cggatcgttg gtgtaccccg
      661 aattccagac cacgtacaga tcatcgccga tgaccggcgt ccaatggaag cggagatcga
      721 aatcgctgcg atgcagcgca ttctcgtatt gcgcgaaggc gagaaaatcc atgcgcgtcg
      781 taaacgcgta ctcgacacgc ccggtggact ggatcgccac gaagtgggca gccggcagcg
      841 tgacatcggt ccgggacacg ccggcaccga ggatgagatg cccgctcgat cgccacgtta
      901 ggccgccatc ggtctccgtg ctgtgcccgt cgtaaaacgt tccggtcgag aacaacgccg
      961 tcgcgctgat cggccggccg tgcgaagact gaaactgtgc gcccgcctgc gtccaccagt
     1021 accggccgcg ctgaatcgcg acgccccggg ccaagctgaa cgtgtcggtc ggtgcatcca
     1081 ggtcgcgaat gatcaccacc tggaagtcat ccccgctct
//
LOCUS       DASUCJ010000528         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1274171, whole genome shotgun sequence.
ACCESSION   DASUCJ010000528 DASUCJ010000000
VERSION     DASUCJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274171"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFU90_06280"
     CDS             <1..321
                     /locus_tag="VFU90_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479420.1"
                     /translation="FVSGESDQLTPAMRRGLLRSLDEARVGARCGCGQPDCRSFRIAG
                     ANPTRDSRRVRFIVHGELSVICDEAGNLQRVEWLVQAPDPTRRHYEFTPDGCIERAIC
                     PFPF"
     gene            412..858
                     /locus_tag="VFU90_06285"
     CDS             412..858
                     /locus_tag="VFU90_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5479421.1"
                     /translation="MMRLSIPAFVTIVLVGAAAAIGASAPATFTPAGMAWVAGTGTAK
                     GTSQVILVGHPDKTGTAVIRVKMPDGYVNQPHYHATAEYITVIQGALLFGTGDTVDKA
                     TATLLPAGSFIEVPAGVHHWSVTKGITIEQVDGEAPLNNIPIKHSM"
     gene            complement(934..2208)
                     /locus_tag="VFU90_06290"
     CDS             complement(934..2208)
                     /locus_tag="VFU90_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5479422.1"
                     /translation="MDECRTVLRGARIFDGKNASPIEGRDVLVVGALIKDLIPAGHAV
                     EGAREIDCAGKFVMPGLIDAHWHAILAAAPLPVVMAGSVPYLYLLAAQEAERTLLRGF
                     TTVRDLGGPTFALKRAIDEARFAGPRIYPSGGMLSQTSGHGDFRLPNELGPASHCAIP
                     MAEAAGISVLADGVPAVLRGAREQLMQGASQIKIMAGGGVASAFDPIDTLQYTPDEMR
                     AAVAAAADFGTYVCAHVYTAAGITRCLDNGVKSIEHGQLADEESMRRIADAGAFWSVQ
                     PFLNDEDANPLATEQQRRDSEAIAQGTVRAIELAKKHGVKTAFGTDILFSPAGTRTQG
                     KQLTKFARWFDNADVLRLATSNAAELLALSGARDPYPAALGVIEGGALADVLVVDGDP
                     LRDIGVLANSERHLQLIMKDGRIYKDAMTTGS"
BASE COUNT          419 a          842 c          649 g          398 t
ORIGIN      
        1 ttcgtcagcg gcgaaagcga ccagttgacg cccgcgatgc gccgcggtct cctgcgttcg
       61 ctcgacgaag cacgcgtcgg cgcgcgatgc ggctgcgggc agcccgactg ccgaagcttc
      121 cgcatcgccg gcgcgaaccc gacgcgcgac tcccgccgcg tacgctttat cgtccacggc
      181 gaactatcgg taatctgcga cgaagccggg aatctgcagc gcgtcgaatg gctcgttcaa
      241 gcccccgacc ccactcgccg tcactacgag ttcacgcccg atggctgcat cgaacgcgcc
      301 atctgcccct ttcccttcta actgcggcgt ttcgcatcgc aaagcgttcc cgggagggcg
      361 ttcccaacgg aacactgaac gcgccgaaca ctccagaaag gagctcgttt catgatgaga
      421 ctctctatac cggcattcgt cacgatcgtt ttggtcggcg cggctgcagc gatcggagca
      481 tcggctcccg cgacgttcac gcccgccggc atggcttggg tggccggaac cggcaccgcg
      541 aagggcacgt cgcaggtcat tctcgtcgga cacccggaca aaacgggcac ggcagtcatc
      601 cgcgtcaaaa tgcccgacgg ctacgtcaac cagccccact atcacgcaac cgcggaatac
      661 attaccgtaa ttcagggagc gctgctgttt gggaccggcg ataccgtcga caaggcaacg
      721 gcgacgcttt taccggccgg ctcgttcatc gaggttcccg ccggcgtcca tcactggtcg
      781 gttaccaagg gcatcacgat cgaacaagtc gacggcgagg ccccccttaa caacatcccg
      841 atcaaacact cgatgtaagc gtttcccaaa acgctactcc agccggttcg gctttcggat
      901 gcgagccgaa cggctggaaa cacgatcccg gagctaggaa cccgtcgtca tcgcatcttt
      961 atagatgcgt ccgtctttca taatcagctg cagatggcgc tccgaattcg ccagcacgcc
     1021 gatatcgcgc agcggatccc cgtcgaccac caacacatcc gccaatgctc ccccctcgat
     1081 aacccccaac gccgccggat acggatcgcg cgcgcccgac agcgccaaga gctccgctgc
     1141 gttcgacgtc gccaagcgca gcacgtcggc gttgtcgaac cagcgcgcaa acttcgtcag
     1201 ctgcttgccc tgcgtgcgcg tgccggccgg gctaaacaga atgtcggtgc cgaacgctgt
     1261 cttcacgccg tgcttcttcg ccagctcgat cgcccgcacg gttccctgcg cgatcgcctc
     1321 gctgtcgcgg cgctgctgct cggtcgccag cggattggcg tcttcgtcgt tgagaaacgg
     1381 ctgcacgctc caaaacgcgc ctgcgtcggc tatccgccgc atcgactctt cgtcggccag
     1441 ttgcccgtgc tcgatcgact tcacgccgtt atccaaacac cgcgtaatcc cggcggccgt
     1501 gtacacatgc gcgcacacgt acgtgccgaa atccgccgcc gccgcgaccg ccgcgcgcat
     1561 ctcgtcgggc gtgtactgca gcgtgtcgat cggatcgaac gccgatgcca ccccgccgcc
     1621 cgccatgatc ttgatctggc tcgcaccttg catcagctgc tcgcgcgcgc cgcgcaacac
     1681 cgccggtacg ccgtcggcca gaacggaaat gccggccgcc tccgccatcg gaatcgcgca
     1741 gtgcgacgcc ggcccgagct cgttcggcaa tcggaaatcg ccgtgtcccg aggtctgcga
     1801 cagcatcccg ccgctcggat agatccgcgg cccggcaaac cgggcttcgt cgatcgcgcg
     1861 cttgagtgcg aacgtcggcc cgcccaaatc gcgcaccgtc gtaaagccgc gcaacagcgt
     1921 gcgctccgct tcctgcgccg ccagcagata aaggtacgga accgatcccg ccatcaccac
     1981 cggcagcggc gcggcggcca aaatcgcatg ccagtgcgcg tcgatcagcc ccggcatcac
     2041 gaacttgccc gcgcagtcga tctcccgtgc gccctcgacc gcgtgaccgg ccggaatcaa
     2101 gtccttgata agcgcgccga cgaccagaac gtcgcgtcct tcgatcggcg acgcattctt
     2161 gccgtcgaaa atccgcgcgc cacgcagcac cgtccggcat tcgtccattg cttgcaccgc
     2221 ctagcctatc gctttaccct ccgcatttcc tggcggtcgc gtcatggccc gaataccaag
     2281 gagttcagtt gccagatgta tccgtgga
//
LOCUS       DASUCJ010000529         1323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_128164, whole genome shotgun sequence.
ACCESSION   DASUCJ010000529 DASUCJ010000000
VERSION     DASUCJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128164"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..715
                     /gene="hisH"
                     /locus_tag="VFU90_06295"
     CDS             101..715
                     /gene="hisH"
                     /locus_tag="VFU90_06295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU5479423.1"
                     /translation="MQPKVVIVDYGAGNLRSVSRAVAHVGYEPSITGDPHDLDDAHAV
                     ILPGVGAAADTMRNLRESGMEAPVRDFIASGRPFFGVCMGQQALLSVSEEGGEHQCLG
                     IIPGRVVRLPKGQKVPHMGWNEVRQRTPHPIFEGIPDASYFYFVHSYVPAPDDPTVVV
                     GETDYGVTFASVLARDNIVATQFHPEKSGEMGLRMYKNFLSQLD"
     gene            complement(1068..>1323)
                     /locus_tag="VFU90_06300"
     CDS             complement(1068..>1323)
                     /locus_tag="VFU90_06300"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5479424.1"
                     /translation="TRGLMAGHVAPEAFVGGPIAAVRDGDIISFDIPNRRLDVEISDD
                     EMAARLRDWRAPAPRYTAGVMAKYAAEVSSASEGAITRAP"
BASE COUNT          224 a          436 c          456 g          207 t
ORIGIN      
        1 ggagatcatg tcggtgagaa cggtaggggc ccaaagcttt gtgcccctac ggctacagtg
       61 ccggatctgc gtggcggacc acggctgtta gactgcacct atgcagccga aggtcgtcat
      121 cgtggactac ggcgccggga acctgcgcag cgtctcccgc gcggtcgcgc acgtcggcta
      181 cgagccgtcg atcacgggcg acccgcacga cctcgacgac gcccacgcgg tgatcctgcc
      241 gggcgtcggc gccgccgccg acacgatgcg caacctgcgc gagagcggca tggaggcgcc
      301 ggtgcgcgac ttcatcgcca gcgggcggcc gttcttcggc gtctgcatgg gccagcaggc
      361 gctgctctcc gtcagcgaag agggcggcga gcaccagtgc ctcgggatca tccccggccg
      421 cgtcgtccgc ctgccgaagg gccagaaggt gccgcacatg ggctggaacg aggtccggca
      481 gcggacgccg cacccgatct tcgaaggcat cccggacgcg agctacttct acttcgtgca
      541 cagctacgtg cccgcgcccg acgacccgac ggtggtcgtc ggcgagacgg actacggcgt
      601 gacgttcgcg tcggtgctgg cacgcgacaa catcgtcgcg acgcagttcc acccggagaa
      661 gtccggcgag atgggcctgc ggatgtacaa gaactttctg tcgcagctcg actgacacgc
      721 gacggaacca cagatgtcac agatgggcac agatgggcac agatgggtcc tgggctatcc
      781 gcagatgacg cagataggcg cagatgattc gagttgggcg cgaacggcgt atcgaacatg
      841 gaatcagcac gactcgtttc gaacgtcgag cgcggctacg acaaccgcgt ccgcacagcg
      901 tttctctcgt agttgagctg aaccacagat gtgacagatg ggtacagatg gcgtctgggg
      961 ctgatccgca gatgacgcag atgggcgcgg atgaatgtgc ggggcgcgtg ccgctgggcc
     1021 aacgccttcc gacctctgtc ctctgacctc cggcacctcg gcgacgtcta cggcgcgcgg
     1081 gtgatcgcgc cttcgcttgc gctcgagacc tcggcggcgt acttggccat gacgccggcg
     1141 gtgtaacgcg gcgccggggc gcgccagtcg cgcaggcgag ccgccatctc gtcgtcgctg
     1201 atctcgacat cgagccggcg gttcgggatg tcgaagctga tgatgtcgcc gtcgcgcacc
     1261 gcggcgatcg gcccgccgac gaacgcctcc ggcgccacgt gcccggccat caggccgcgc
     1321 gtc
//
LOCUS       DASUCJ010000530         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1197866, whole genome shotgun sequence.
ACCESSION   DASUCJ010000530 DASUCJ010000000
VERSION     DASUCJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU90_06305"
     CDS             complement(<1..234)
                     /locus_tag="VFU90_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479425.1"
                     /translation="MLADPNADGQRIYDTTKHLSVDIGPRVAGSDGEQAARDYLEPML
                     RSYGYDVTEQAFPFDATAYLPARVDLASDAYGAI"
     gene            complement(393..842)
                     /locus_tag="VFU90_06310"
     CDS             complement(393..842)
                     /locus_tag="VFU90_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479426.1"
                     /translation="MSKPENGQDRIHREIEELLDRLDNFVPEERLATKIRNRARRKEP
                     DSPGILKRIGARFGRVSLGQLMIAGFILIIAAYFLRGPLGGLTPWLIGHGLVMTGAAL
                     VLSIVRGGNSRTIDGSVEKRWRGQVIRYSTGPSAADRIRDWFRRRRR"
     gene            950..>1657
                     /locus_tag="VFU90_06315"
     CDS             950..>1657
                     /locus_tag="VFU90_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479427.1"
                     /translation="MAAAGRIYGAFTPRCTAALPSPRTLPLLEMERERREGDQPMRIS
                     TKALALGATLVIIAAATFGAFRYASAQEGTPSPDATAAASSTPTRRQQAVYAFIDKVA
                     KNLNVSSDQLKSAVKDAALQTVDEQVAAGKLTQAQGDKIKAAINSGKYPQLARLFRGR
                     QAILTQVRRGIVVSSAKAIGIAPKDLASELKSGKSIADVAGEHNVSLDTVKSQITSAF
                     KTKLDELVKGGKITQAQE"
BASE COUNT          285 a          538 c          578 g          256 t
ORIGIN      
        1 gatcgcgccg tatgcgtcgg aggcgaggtc gacgcgcgcc ggcaggtatg cggtcgcgtc
       61 gaaggggaac gcctgctcgg tcacgtcata gccataggag cgaagcatcg gttcgaggta
      121 atcgcgcgcg gcctgttctc cgtcgctgcc ggcgactcgg ggtccgatgt ccaccgagag
      181 atgcttcgtg gtgtcgtaga tgcgctgccc gtccgcgttc gggtccgcga gcacgtcggg
      241 cgtggacgca gccggcgtgg cgctcgccgc cggtgtgaca ccgtacggcg tcgcggtgtt
      301 cgcgtgcgag ccgccgctac tgcacgcgac ggcgagcgtt gcgacgatcg cgatgccgat
      361 gctgaggatc gagccggaac gcggaatgac cgtcagcggc gccgcctgcg gaaccagtcg
      421 cgaatgcggt ccgcggcgga cggcccggtc gagtagcgga tcacctgccc gcgccagcgc
      481 ttctcgacgg agccgtcgat cgtgcgggag ttgccgcctc ggacgatcga cagcaccaag
      541 gcggcgccgg tcatgacgag gccgtgcccg atgagccacg gcgtgaggcc gccgagcggt
      601 ccgcgcagga agtacgcggc gatgatgagg atgaagccgg cgatcatcag ctgaccgagc
      661 gagacgcgcc cgaagcgcgc tccaatccgc ttcaggatgc ccggcgagtc gggctcctta
      721 cggcgcgcgc ggttgcggat cttcgtcgca agccgctctt ccggcacgaa gttatcgagc
      781 cggtcgagca actcctcgat ctcgcgatgg atccgatcct ggccgttctc gggtttgctc
      841 atgagggctc cgggtgctga gtctattgta gccgcgtccc cgcagacccg tccagaatca
      901 gatacgcctt ccgacgctag ccggttcacc cggtgggctc gtggccactt tggccgcggc
      961 cggccgaatt tacggagcgt ttacgccgcg ttgcacggcg gcgctgcctt cgcctcgtac
     1021 gctgccgttg ttggagatgg aacgggagag gagagaagga gaccaaccca tgagaatctc
     1081 gacaaaggca ctggcgctcg gcgccacact cgtcatcatc gccgccgcca cgtttggtgc
     1141 gttccgctac gcctcggcac aggaaggcac gccctcgccc gacgcgactg ccgccgcatc
     1201 ttcgacgccg accaggcggc aacaggccgt ctatgcgttc atcgacaagg tcgcgaagaa
     1261 cctcaacgtc tcgtccgacc agctgaagtc cgcggtgaag gacgccgcgc tgcaaacggt
     1321 cgacgagcag gtcgccgccg gcaagctgac gcaggcgcag ggcgacaaga tcaaggccgc
     1381 gatcaacagc ggcaagtacc cgcagctcgc gcgcctcttc cgtggccgcc aggcgatcct
     1441 gacccaggtg cggaggggca tcgtcgtgtc gtcggcgaag gcgatcggca tcgcgccgaa
     1501 ggatctcgcg tcggagctga agagcggcaa gtcgatcgcc gacgtcgcgg gcgagcacaa
     1561 cgtgtcgctg gacacggtga agtcgcagat cacgtccgcc ttcaagacga agctcgacga
     1621 actggtcaag ggtggcaaga tcacgcaggc gcaggag
//
LOCUS       DASUCJ010000531         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_831017, whole genome shotgun sequence.
ACCESSION   DASUCJ010000531 DASUCJ010000000
VERSION     DASUCJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831017"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1455
                     /locus_tag="VFU90_06320"
     CDS             19..1455
                     /locus_tag="VFU90_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479428.1"
                     /translation="MRKRALAASVALVAAVAAGCIAAETAASRSSAPSGDIHKIAHVV
                     IIMQENRSFDTYFGTFPGADGIPMKEGVPAVCVPSPAGGPCVRPFYDPKDRNRGGPHN
                     FHASQVDVNGGKMDGFLNAIKGLARCTTYGDPNCAGRARTRDVMGYHDERQLANYWKY
                     ARDFVLQDHLFENVASWSLPQHLFMVSEWSAKCDQLHVAMSCTNEPESPDYPPDFRRN
                     VPGAHGPGRPDYAWTDLTYLLHRKRVSWAYYVMSGTQPDCANDLESCKAMSQSYRTPG
                     IWNVLPAFDTVRQDGELGNVRDLRQFYADAQSGRLPSVVWICPAGKYSEHPSAAISNG
                     QAYVTGLINTIMRSPEWNSTVILLSWDDWGGFYDHVPPPRVDENGYGIRVPGIVISPY
                     ARKGFIDHQVLSHDAYVKFIEDDFLGGERIDPKTDGRPDRRPTVREDSPQLGDLRADL
                     DFSQAPRPPEILPNATIWNGRTADPESP"
     gene            complement(1470..>2663)
                     /locus_tag="VFU90_06325"
     CDS             complement(1470..>2663)
                     /locus_tag="VFU90_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479429.1"
                     /translation="AMFLISDTGGGHRSAANAITAALDEIGTPHPFEHRVEDVAAHCS
                     FPLTQLGLGYSMALRYAPPVYGALYYATNGRRRYKALIRFCEPLYRERLRDLFVDYRP
                     DVIVSVHPLLNHAALRARADADMQQVPIVTVVTDLGKVHESWLVADADAVVVPAREVY
                     QRALSRGVPPARLRLLGHPIHPKFDDVTGTPAELRAQLGLPTDRMIVMLMAGGEGGGK
                     LLSTTLALARARLPVHLVVVCGRNDHLQQKLQEMAASLPTPMHVLGFTDNIPEYFRAA
                     DLLVTKAGPGTLAEANAAQLPVVVYDYVPGQERGNVDFVRHNGLGAVAIHGASEVVAA
                     VRSMIRTPERLTAIRHNQEIVAPRRSSRRIAALIARIAETGTIPPQQDLPAAEPAALR
                     QAAGV"
BASE COUNT          486 a          874 c          882 g          421 t
ORIGIN      
        1 tggaggtgga acgaaactat gcgtaaacgt gccctcgccg catcggtcgc cctggtggcg
       61 gccgtcgcgg ccggttgcat cgccgccgaa acggccgctt cgcggtcgag tgcgccgagc
      121 ggcgacattc acaagatcgc gcacgtcgtc atcatcatgc aagagaaccg ctccttcgat
      181 acctatttcg gcaccttccc cggcgcggac gggatcccga tgaaggaggg cgtcccggcc
      241 gtctgcgttc cgtcgcccgc cgggggcccg tgcgtccggc cgttctacga tccgaaggat
      301 cgcaatcgcg gcgggccgca caacttccac gcttcgcagg tcgacgtcaa cggcggaaag
      361 atggacggct ttctgaacgc gatcaaaggc ctcgcacgct gcacgaccta cggcgacccg
      421 aactgcgccg gccgcgcgcg cacgcgcgac gtcatggggt accacgacga acgtcagctc
      481 gccaactact ggaagtacgc gcgcgacttc gtgctgcagg accatctctt cgaaaacgtc
      541 gcctcgtgga gccttccgca gcacctgttc atggtttccg agtggtcggc aaaatgcgac
      601 cagctgcacg tcgcgatgag ctgcacgaac gaacccgaga gccccgacta tccgccggac
      661 tttcgccgca acgttcccgg cgcacacggc cccggccgcc ccgattacgc ctggaccgac
      721 ttgacgtatt tactgcatcg caaacgcgtc agctgggcgt actacgtgat gagcgggacg
      781 cagccggatt gcgccaacga tttggaatcg tgcaaggcga tgagccagag ctatcggacg
      841 ccgggaatct ggaacgtgct tccggcgttc gataccgttc gccaagacgg cgagctcgga
      901 aacgttcgcg acctgcgcca gttctacgcc gacgcgcaat ccggacgtct cccgagcgtc
      961 gtctggatct gtccggctgg aaagtacagc gagcacccgt cggccgcgat cagcaacggc
     1021 caggcctacg tcaccggtct aatcaacacg atcatgcgca gccccgagtg gaacagcacg
     1081 gtgattctgc tcagctggga cgactggggc ggcttctacg atcacgtacc gccgccgaga
     1141 gtcgacgaga acggttacgg gatccgagtt ccgggaatcg tgatcagccc gtacgcgcgt
     1201 aaaggcttca tcgatcacca agtgctcagc cacgacgcgt acgtcaagtt catcgaggac
     1261 gacttcctcg gcggcgagcg catcgacccg aaaaccgacg gacgcccgga tcgccggccg
     1321 accgtccgcg aggactcacc gcagctcggc gacttgcgcg ccgacttgga tttctcgcaa
     1381 gctccgcgcc cgccggagat tctgcccaac gcgacgatct ggaacggccg aacggccgat
     1441 cccgaatcgc cgtagggtac gaaaggcgct taaacgccgg cggcttgacg gagagccgcc
     1501 ggttcggcgg cgggcaagtc ttgctgcggc gggatcgtgc cggtctcggc gatccgcgcg
     1561 atcaaggcgg cgattcggcg cgagctgcgg cgcggtgcga cgatttcctg attgtgacgg
     1621 atcgccgtta gccgctcggg ggtgcgaatc atggaacgca cagccgcgac cacctcggaa
     1681 gctccgtgaa tggcgaccgc tcccaacccg ttgtgccgaa cgaagtcgac gtttccgcgc
     1741 tcctggccgg gaacgtagtc gtacaccacg accggcagct gggcggcgtt tgcctcggca
     1801 agcgttccgg ggccggcttt ggtaaccagc aagtcggccg cgcggaaata ctcggggata
     1861 ttgtcggtga agccgagcac gtgcatcggc gtcggcaggc tcgcggccat ttcttgcagc
     1921 ttctgctgca aatggtcgtt acggccgcaa acgacgacga ggtgaacggg aagacgcgcg
     1981 cgggccagcg ccagcgtcgt cgaaagcagc tttccgccgc cttcgccgcc ggccatcagc
     2041 atcacgatca tccggtcggt ggggagaccc agctgcgcgc gcagctcggc cggcgtcccg
     2101 gtcacgtcgt cgaatttcgg atgaatcgga tggccgagca gccgcaggcg cgccggcggc
     2161 acgccgcgcg agagcgcccg ttgataaact tcccgggcgg ggacgacgac cgcgtcggca
     2221 tcggcgacca gccacgactc gtggaccttt cccaagtcgg tgacgaccgt cacgatcggc
     2281 acctgctgca tgtccgcgtc ggcccgcgcg cgcagggcgg cgtgattgag caacgggtgg
     2341 accgagacga tcacgtcggg gcgatagtcg acgaacaagt cgcgcagccg ctcgcggtag
     2401 agcggttcgc aaaagcggat cagcgcctta taccgccgcc gtccgttggt ggcgtagtag
     2461 agcgcgccgt acaccggagg ggcgtagcgc aaggccatgc tgtagcccag cccaagctgg
     2521 gtcaacggga aggagcagtg ggcggcgacg tcttcgacgc ggtgctcgaa ggggtggggc
     2581 gtcccgatct catccagcgc ggcggtaatc gcgttggcgg cgctgcgatg gccgccgccg
     2641 gtatccgaga tcaggaacat ggc
//
LOCUS       DASUCJ010000532         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_112756, whole genome shotgun sequence.
ACCESSION   DASUCJ010000532 DASUCJ010000000
VERSION     DASUCJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112756"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFU90_06330"
     CDS             complement(<1..523)
                     /locus_tag="VFU90_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479430.1"
                     /translation="MKMRLGAAVAAALVFLPAARPPIAMAQATAITGTWSAERTATPG
                     RLHVEFSSTDADGKSDISSSFDQDGAQFGLTAAQLDSPGTHVAFTFAREAGSFACQGW
                     VAHGKGGGPFTFTPSAAYAAGIRSRGYSGLTSRDQMAAAVLDLTTGYADAIASAGYPH
                     LAFSKLVAFRDLKI"
     gene            complement(520..>1110)
                     /locus_tag="VFU90_06335"
     CDS             complement(520..>1110)
                     /locus_tag="VFU90_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479431.1"
                     /translation="TNTVTAVVTAPSGSPAQTFVQFRAPEPKSFVAIGGSAAGTVAQS
                     VVVAANSPDYIDSLASVGYTNLSVDDLVRLKAVGVTAEYIRGLQDAGFSHPSVRDLIQ
                     MRAMDVDGAYIRAMRAYFGSAVTLREVAQLHALGVTEDYLRGMASAGYPNLKVREASE
                     LRAMGIDPTFVASAASHGMHNLTVEQLVRLKASGVL"
BASE COUNT          179 a          379 c          365 g          187 t
ORIGIN      
        1 cgatcttcag gtcacgaaac gcgacgagct tgctgaacgc caggtgcgga tagcccgccg
       61 atgcgatcgc gtcggcgtag ccggtcgtga ggtccagcac cgcggcggcc atctgatcgc
      121 gcgaggtgag cccgctatat ccgcggctcc gtatgccggc ggcgtacgcg gcgctcggcg
      181 tgaaagtgaa cggcccgccg cctttgccgt gcgcgaccca gccttggcac gcgaacgatc
      241 ccgcttcgcg tgcgaacgta aacgcgacgt gcgttccggg tgaatcgagc tgcgcggcgg
      301 tcagcccgaa ttgggcgccg tcctggtcga acgaactgct gatgtcggat ttcccgtccg
      361 catcggtgct gctgaactcg acgtgcagcc tgcccggcgt cgcggtgcgc tccgccgacc
      421 acgttccggt aatcgccgtc gcctgcgcca tcgcgatcgg cgggcgcgct gccggcagga
      481 agaccagtgc cgcggcgacg gccgcgccca aacgcatctt catagcacgc cgctcgcttt
      541 cagccggacg agttgctcga cggtaaggtt gtgcatcccg tgcgaggccg ccgatgcgac
      601 gaaggtgggg tcgattccca tcgcccgcag ttcgctggct tcccgcactt tcagattcgg
      661 gtaacccgcg ctcgccatcc ctcgcaggta gtcttcggtc acgccgagcg cgtgcagctg
      721 cgcgacttcc cgcaaggtga ccgcgctgcc gaagtatgcg cgcatcgcgc gaatgtaggc
      781 tccgtcgacg tccatcgcgc gcatctgaat cagatccctg accgagggat ggctgaagcc
      841 ggcgtcctgc agcccacgga tatactcggc ggtcacgccg acggctttca gccggaccaa
      901 atcgtcgacc gaaaggttcg tgtagccgac gctcgccaga gagtcgatgt agtcgggcga
      961 gttggcggcg acgaccaccg actgcgctac cgtcccggcg gcactgccac cgatcgcgac
     1021 gaagctcttc ggctccggtg cacgaaactg cacgaaggtc tgcgcgggac tgcccgacgg
     1081 agcggtaacg accgccgtca ccgtattggt
//
LOCUS       DASUCJ010000533         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_678139, whole genome shotgun sequence.
ACCESSION   DASUCJ010000533 DASUCJ010000000
VERSION     DASUCJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678139"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1180
                     /locus_tag="VFU90_06340"
     CDS             251..1180
                     /locus_tag="VFU90_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEU5479432.1"
                     /translation="MKLHGNAKLTPVQRRLLVRRVLEERWTIADASDAAGVSERTAYR
                     WVRRWRDGDYELNDRSSAPKRVYRTPARTVELIETLRRTRMSSPKIAAKLRMAVSTVC
                     AVLARIGLNRISKLGPPEPPNRYERRRPGDLIHVDIKKLGRFVRPGHRVTGRDAKGSR
                     ANIGHGWDFVHVAIDDHSRVAYVEILDSERAEDATAFLERAIEWFADLGVRVRSVMTD
                     NGSCYVSRDWAATCKRGRITHLRTRPNRPRTNGKAERMIQTLQREWAYAAVYRTSTHR
                     NRALQPWMRFYNHRRPHGSLGHQPPASRLPAAA"
BASE COUNT          266 a          462 c          434 g          215 t
ORIGIN      
        1 cgacgctgcg aaggcgatct gtgccgtctg cccggtgcgc gaagcgtgcc tcgagcacgc
       61 gctcgagacg cgtccgcccg agggtgtgtg gggtggtctg accccgatcg aacggcaccg
      121 gctgatccgg cgccggcaga aggccgcgcg caaagctcgc gccgcctagt tgtagttccc
      181 aatgacgttc gtcagtacgg gtctccttgg agactggggg tgctgaagcc tccaactcca
      241 aggagaaccc gtgaaactgc acggtaacgc caagctcacc cctgttcagc ggcggctgtt
      301 ggtccgtcga gtgctcgagg agcgttggac gatcgctgac gcttctgatg cggccggtgt
      361 ttcggaacgc acggcatatc ggtgggtgcg gcgctggcga gatggcgact acgagctcaa
      421 cgatcggtcg tccgctccga agcgggtata tcgcacgccg gcgaggactg tggagttgat
      481 cgagacgttg cgacgcacgc ggatgtcgtc accgaagatc gccgcgaagt tgcgaatggc
      541 ggtctccacg gtgtgcgcag tgctcgcccg catcggactg aaccgcattt cgaagctcgg
      601 tccgcccgaa cctcccaacc gttacgagcg gcgccggccc ggtgatctca tccacgtcga
      661 tatcaagaaa ctcggccgct ttgttcgccc tggtcaccga gtcactggcc gagacgccaa
      721 aggatcgcga gcgaacatcg ggcatggatg ggatttcgtg cacgtcgcga tcgacgatca
      781 cagccgcgtc gcgtacgtcg agatcctcga cagtgaacgc gccgaagacg cgaccgcgtt
      841 cttggaacgc gcgatcgagt ggttcgcgga tctcggcgtc cgcgtccgct cggtcatgac
      901 cgacaacggc agctgctacg tctcacgcga ctgggccgcg acctgcaaac gaggacgcat
      961 cacgcatcta cgcacacgac cgaaccgacc gcgcaccaac ggcaaagccg agcgcatgat
     1021 ccaaacactg caacgcgagt gggcctacgc cgcggtctac cggactagca cacaccgcaa
     1081 ccgagcgctc caaccctgga tgcgcttcta caatcaccgc cgaccccacg gcagcctcgg
     1141 tcaccaacca cccgcaagcc gcctacccgc ggcagcctga ccaacgtgcc taggaactac
     1201 aactagctca ttggtttaca accaatgcgg cgactccagg ctcgaagcgc gtccgagcgg
     1261 gagcgtcacc gaatcctgcg gcgtcgcagg aaggtcgcca cgcaggcata gatcggcgcc
     1321 ggctcggcgc tgaagccgca gccaatgccg gcgatcacgt cggcgagcgg cgccgcc
//
LOCUS       DASUCJ010000534         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_800693, whole genome shotgun sequence.
ACCESSION   DASUCJ010000534 DASUCJ010000000
VERSION     DASUCJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800693"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFU90_06345"
     CDS             complement(<1..1056)
                     /locus_tag="VFU90_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479433.1"
                     /translation="MRIKPAYLAAAVAFAIAGLVSHLRPSPYDNYVLLAQAFLHGHAW
                     IDWPGAYIDALGYGGRYYVIEAPLPAVLLLPAVALFGTSANQTLLAAVLCAIAVGAAW
                     NLGERFGLSPLKNAWICAFLLAGTDLLWCAMLGDVWFVAHVAAACFTLLALAECFGRR
                     RGWLVALWAACAFESRFSLIVVVPVYLYLLLQDANAPPVLDARRRGVLLGFAAVLIPV
                     AVLWVWYNRARWGTWNDVGYTLWYHQDQAGFPTGSPFRLEYLPYQLQSFFVQWPTWLG
                     AFPFFRPEYSGVALTWTSPALVLALLARTPPRWVAALWVAALLAAAPNFLYYVNGFAQ
                     FGMRHALDFEPFLVALMM"
     gene            complement(1059..>1272)
                     /locus_tag="VFU90_06350"
     CDS             complement(1059..>1272)
                     /locus_tag="VFU90_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479434.1"
                     /translation="IHFHTQGAGAHGTGEVQYAAFGPYWVPVVASVDASVGGKPARER
                     ITWGDYRFPEDLPPSTFQAPRPLPPH"
BASE COUNT          282 a          423 c          416 g          151 t
ORIGIN      
        1 catcatcaac gccaccagaa acggttcgaa atccagcgcg tgacgcatgc cgaactgcgc
       61 gaacccgttc acgtaataca gaaagttggg cgcggcggcg agcagcgccg cgacccagag
      121 cgcggcaacc cagcgaggcg gcgtccgggc gagcagcgcg aggacgagcg ccggcgacgt
      181 ccaggtgagg gccacgcccg aatactccgg acggaaaaac ggaaacgcgc cgagccacgt
      241 cggccattga acaaagaacg actggagttg gtacggcaag tactcgagcc ggaacggcga
      301 tccggtcgga aagccggcct gatcttggtg ataccatagc gtgtacccga cgtcgttcca
      361 agttccccag cgcgcgcgat tgtaccacac ccagagcacc gcgacgggaa tcaacacggc
      421 tgcaaacccg agcagcacgc cgcgtcggcg cgcgtcgagc accggcggcg cgttcgcatc
      481 ctgcagcagc aggtacaggt acacgggcac gacgacgatc agcgaaaagc gcgattcgaa
      541 cgcacacgcc gcccatagcg cgaccagcca gccacggcgc ctgccgaagc actccgcgag
      601 cgccagcaac gtgaagcacg cggccgcaac gtgcgccacg aaccacacgt cgccgagcat
      661 cgcgcaccat agcaggtcgg tgcccgccag caggaacgca caaatccacg cgttcttcaa
      721 cggcgagagc ccgaagcgtt cgccgagatt ccaagcggcg ccgacggcaa tcgcgcacag
      781 gacggcggcc agcagcgttt gattcgcgga cgtaccgaag agcgcgaccg ccggcagcag
      841 caagactgcc ggaagcgggg cttcgatgac gtagtagcgc ccgccgtatc cgagcgcgtc
      901 gatatacgcc cccggccagt cgatccaagc gtgaccgtgc aaaaacgctt gcgccagcag
      961 aacgtagttg tcgtacgggc tcggccgtaa gtgcgaaacg agccccgcga tcgcgaaagc
     1021 gacggcggcg gcgaggtacg cgggcttaat gcgcaacgtt agtgcggagg taacggacgc
     1081 ggcgcttgaa aggtcgacgg cggcaggtct tcgggaaacc ggtagtcgcc ccacgtgatg
     1141 cgttcgcgag cgggcttgcc cccgacgctc gcgtccaccg aagcgactac cggcacccaa
     1201 tacgggccga aggccgcgta ctgaacttcg ccggttccgt gtgcgccggc accctgggtg
     1261 tgaaagtgga tc
//
LOCUS       DASUCJ010000535         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1167307, whole genome shotgun sequence.
ACCESSION   DASUCJ010000535 DASUCJ010000000
VERSION     DASUCJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167307"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFU90_06355"
     CDS             <1..257
                     /locus_tag="VFU90_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5479435.1"
                     /translation="MACHGPDGRGMAGAGYPQLAGQWADYVQAKLKDFKAGTAWGDDN
                     NAKIMPEIARHLGDSDIAALASYVEGLHSAAADAKATASR"
     gene            360..1031
                     /locus_tag="VFU90_06360"
     CDS             360..1031
                     /locus_tag="VFU90_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEU5479436.1"
                     /translation="MLKRFVAVLFGLAVLPLAHAQALQYEAGKQYFLVEPPQPTTTGD
                     KVEVLEVFSYACPACNAFQSAANKIKSELAANAQMAYLPAAFRADEDWPVFQRAFYAA
                     QALGLVEKTHDAMFDAIWKEGSLKISDPVTHKLVQPMPTIDDVAKFYAKYGVKAEDFV
                     GTANSFAVNAKIKRADAQVKAYGVDSTPTIIVNGKYRITGASAGGMDKVPALVTYLVG
                     LESHK"
BASE COUNT          196 a          348 c          356 g          172 t
ORIGIN      
        1 gcatggcctg ccacgggccc gacggccgcg gcatggccgg cgccggctat ccgcagctcg
       61 cgggccagtg ggccgactac gtccaggcca agctcaagga cttcaaggcc ggcacggcct
      121 ggggcgacga caacaacgcg aagatcatgc cggaaatcgc caggcacctc ggcgatagcg
      181 acatcgcggc gctcgcctcg tacgtcgaag gcctgcactc ggccgcggcc gacgccaagg
      241 cgacggcttc gcgctgaacg caagccgggc tggagcgggt cccatgggcg acgccggctg
      301 aacttggcgc gctccatccg ctccaagccg gtcgctcgat cgtttttatc ggaggcacca
      361 tgttgaagcg tttcgttgct gtgctgttcg gactcgcggt gctgccgctc gcgcatgcgc
      421 aggccctgca gtacgaagcc ggcaagcagt atttcctcgt cgagccgccg cagccgacga
      481 cgaccggcga caaggtcgaa gtgctcgaag tgttctcgta cgcgtgcccc gcgtgcaacg
      541 ccttccagtc ggccgcgaac aagatcaagt ccgagcttgc ggcgaacgcg cagatggcgt
      601 acctcccggc tgcgttccgt gccgatgagg actggccggt gttccagcgc gcgttctatg
      661 cggcgcaggc gctcgggctc gtcgagaaga cgcacgatgc gatgttcgac gcaatctgga
      721 aggaaggctc gctcaagatc tcggatcccg tcacgcacaa gctcgtgcag ccgatgccga
      781 cgatcgacga cgtcgcgaag ttctacgcga agtacggcgt caaggccgaa gacttcgtcg
      841 gcaccgcgaa ctcgttcgcc gtgaacgcga agatcaagcg cgccgatgcg caggtgaagg
      901 cttacggcgt cgactcgacg ccgacgatca tcgtcaacgg caagtaccgg atcactgggg
      961 cgtcggccgg cggcatggac aaggtgccgg cgctggtgac gtatctcgtc ggtctcgaaa
     1021 gccacaagtg aaccttcgga atgccaggcg gtctcgcttc caacgtttga gg
//
LOCUS       DASUCJ010000536         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_770000, whole genome shotgun sequence.
ACCESSION   DASUCJ010000536 DASUCJ010000000
VERSION     DASUCJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770000"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..739)
                     /locus_tag="VFU90_06365"
     CDS             complement(155..739)
                     /locus_tag="VFU90_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5479437.1"
                     /translation="MTRGAICKSVLVAVAAITPMHVRQHGMSSRAPVHYGLGRPATAA
                     DIQAWDVTVMPDGRGLPSGSGTPAQGAAIFAQRCARCHGPTGVEGPFDRLVGREPRQG
                     FPFGRDTSLVRTIGNYWPYSTTVYDYIDRAMPYDAPGSLTPNEIYSLVAFLLWKNEII
                     ADTATMDARTLPRVRMPARDRFVVDNRRGGPEVR"
     gene            complement(736..>1748)
                     /gene="soxC"
                     /locus_tag="VFU90_06370"
     CDS             complement(736..>1748)
                     /gene="soxC"
                     /locus_tag="VFU90_06370"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEU5479438.1"
                     /translation="LTPLQDLHGIITPSDLHYERHHAGVPAIDPHEYKLLIHGMVDRP
                     TVFDLEALKRFPAVSRFHFLECSGNGSSGYTDVRPDQSPQVVDGLTSTSEWTGVSLST
                     LLREVGVHPRATWFLAEGGDAALMARSIPIAKAWDDAMIAYGQNGEALRPEQGYPARL
                     LLPGWEGNTNVKWIRRLEFADRPFMTRDETSKYTDPLPNGTARMFSFVMDAKSIITSP
                     AYPAALTGHGWWDVTGLAWSGRGRISRVDVSTDGGTRWSPAVLEQPVLPKCHTRFTFP
                     WKWDGAEALLMSRATDETGYVQPTLAELRRVRGLGTNYHFNNMRAWRVRRDGSVLFGL
                     EG"
BASE COUNT          280 a          613 c          563 g          292 t
ORIGIN      
        1 cccatgtgat gcgtccatcg cgcgacgcga gcgccgcgtt ctcgatcgca tggatgtcac
       61 cctgcccgcc tgcggcagcg caggtggcca acatcccgat ctctcgtatg acaggcacga
      121 tagtgtctcg acgctccgcg atggcgttgc ccgactaacg aacttcgggt ccgccgcgac
      181 ggttgtccac cacgaagcga tcgcgcgccg gcatgcgcac acgcggaagc gtccgggcgt
      241 ccatggtggc cgtgtcggca atgatctcgt ttttccacag cagaaacgcg acgaggctgt
      301 agatctcgtt cggcgtcaac gaccccggcg cgtcgtacgg catggctcgg tcgatgtagt
      361 cgtagacggt cgtcgaatag ggccagtagt tgccgatggt ccggacgagc gacgtgtcgc
      421 ggccgaacgg aaagccctgc cgcggctcgc gcccaacgag gcgatcgaac ggcccctcga
      481 cgccggtcgg accgtgacag cgcgcgcatc gctgcgcgaa gatggccgcg ccctgggccg
      541 gcgtcccgct gccgctgggg agtcctctcc cgtccggcat cacggtgacg tcccatgcct
      601 ggatgtcggc ggcggtcgcg gggcgcccca ggccgtagtg cacgggcgcc cgggacgaca
      661 tgccgtgctg ccgaacgtgc atcggcgtga tggccgccac ggcgacgagc acgctcttgc
      721 agatcgcgcc cctggtcatc cctcgagccc gaacaggacg ctgccatccc gccgcacgcg
      781 ccacgcccgc atgttgttga aatggtaatt cgtgcctaac cctctcacgc ggcgaagctc
      841 ggcgagcgtc ggctgcacgt atcccgtctc atcggtggcg cgactcatga gcagcgcctc
      901 ggcgccgtcc cacttccacg ggaacgtgaa tctcgtgtgg cacttgggca gaaccggctg
      961 ctcgagcacc gcgggcgacc accgcgtgcc gccgtccgtc gagacgtcga cacggctgat
     1021 ccgccctcgg ccgctccacg ccagaccagt gacgtcccac catccatggc cggtcagtgc
     1081 cgccggatac gccggggacg tgatgatgga cttcgcgtcc atgacgaacg agaacatccg
     1141 cgccgttccg ttaggcagcg gatccgtgta cttcgacgtt tcgtcgcgcg tcatgaacgg
     1201 ccggtcggca aactcgagac gccggatcca cttcacattg gtgttgcctt cccacccggg
     1261 cagcagcaac cgcgccgggt agccctgctc gggtcgcagt gcctcgccgt tctggccgta
     1321 cgcgatcatc gcgtcgtccc aggccttcgc gatcgggatg ctccgcgcca tcaacgcggc
     1381 gtctccgccc tcggcgagaa accacgtggc gcgcgggtgc acacccacct ctcgcagcag
     1441 cgtcgacagc gagacgcccg tccattcact cgtgctcgtt aggccatcca cgacttgcgg
     1501 cgactggtcc ggacgcacgt ccgtatatcc gctcgagccg ttgcccgagc actcgagaaa
     1561 gtggaagcgc gagaccgccg ggaatctctt gagcgcctcg aggtcgaaca ccgtcggccg
     1621 atcgaccatg ccgtgaatca gcagcttgta ttcgtgcgga tcgatggccg gcacgcccgc
     1681 gtgatgccgc tcgtagtgca gatccgacgg cgtgatgatg ccgtgcagat cttgcagcgg
     1741 ggtgagcg
//
LOCUS       DASUCJ010000537         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1426956, whole genome shotgun sequence.
ACCESSION   DASUCJ010000537 DASUCJ010000000
VERSION     DASUCJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426956"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="kdpA"
                     /locus_tag="VFU90_06375"
     CDS             <1..1141
                     /gene="kdpA"
                     /locus_tag="VFU90_06375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU5479439.1"
                     /translation="VVGLFLISQGVPQNFHAYGTLKSIEGFAQSITGGPMASQEVIKE
                     LGTNGGGFVNANSASPNENPNPLTNLVEMLLIFLLGAGLTYTYGTMVKNTRQGWAIFT
                     AMAIVFFAGLALAYSSEAAGNPIVHSLGVAGSNMEGKECRFGAAASALFATVTTDTSC
                     GAVNAMHDSFMPLGGLVPLVNMQLGEIIFGGVGSGLYGMIVFVVLTVFIAGLMVGRTP
                     ELLGKKIERREVQFAILAALVTPVLSLVPTAVAAIVPAGLATLNNSGPHGFSEILYAF
                     TSTNANNGSAFAGLGPNLFYNLTTGVNMMFGRFTVAIPALALAGALAGKKAVPESAGT
                     FTTHSPVFVALLIGVIVIVGALTFLPADALGPIVEHLFVQQGKTF"
     gene            1155..>2865
                     /gene="kdpB"
                     /locus_tag="VFU90_06380"
     CDS             1155..>2865
                     /gene="kdpB"
                     /locus_tag="VFU90_06380"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU5479440.1"
                     /translation="MLPVRRLERRPKARSLFDRAIIVRAIGDSFKKLDPRGQARNPVM
                     FVVEVGALVTSIFFVRDLARHTGTAGFDLSISAWLWFTVLFANFAEAVAEGRGKAQAD
                     YLRRTKSDTTARLLGEDGKEEAVPSTSLRKGDRFVVAAGEIVAADGEVLEGAATVDES
                     AITGESAPVIRESGGDRSAVTGGTRVLSDRIVVRVTANPGESFLDRMIALVEGAQRQK
                     TPNEIALSILLAGLTIIFVIAVATLAPYSIYAGAAQSVTVLIALLVCLIPTTIGGLLS
                     AIGIAGMDRVMQRNVLAMSGRAVEAAGDVDTLLLDKTGTITLGNRQAVEIIPADGIDM
                     QDAARMAYLSSMADETPEGRSIVVLAQAQGSRDGEPEGSTFVPFSAYTRMSGLDLADG
                     SKIRKGAPDSVLAWVREQGGNPSAELSPHIDRIARSGGTPLLLARNAVVLGVIYLKDI
                     LKPNMRERFDRMRAMGIRTVMITGDNPLTAATIAKEAGVDDFLAEATPETKMALIRRE
                     QTSGRLVAMTGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDSKPTKQIDVVEE
                     GKQL"
BASE COUNT          495 a          899 c          955 g          516 t
ORIGIN      
        1 cgtcgtcggc ttgttcctca tttcgcaggg ggtgccgcag aactttcacg cttacggcac
       61 gctgaagtca atcgagggct tcgcgcagtc gatcacgggc ggtccgatgg cttcgcagga
      121 agtcatcaag gagctgggga ccaacggcgg cggtttcgtc aacgccaact cggcctcgcc
      181 caacgaaaac cccaacccgc tgaccaatct cgtcgagatg ctgctgattt tcttgcttgg
      241 cgccggactg acgtatacgt acggcacgat ggttaagaac acgcggcagg ggtgggcgat
      301 cttcaccgcg atggcgatcg tctttttcgc cggattagcc ctggcctact cgtcggaagc
      361 ggccggcaat ccgatcgttc atagcttggg ggtggccggc agcaacatgg aggggaagga
      421 gtgccggttc ggggctgcgg cgtcggcgct gttcgccacc gtcacgaccg acacgtcgtg
      481 cggcgcggtc aacgccatgc acgattcgtt catgccgctc ggcgggttgg ttccgctggt
      541 gaacatgcag ctcggcgaaa tcatcttcgg cggggtcggc agcgggctct acggcatgat
      601 cgtcttcgtc gtcttgaccg ttttcatcgc ggggttgatg gtcggtcgca cgccggagct
      661 gctcggaaag aagatcgagc gccgcgaggt gcagttcgcg atcctggcgg cgctggtcac
      721 gccggtgcta agtctcgtac ccacggcggt cgccgcaatc gttccggcag gcctcgcgac
      781 gctgaacaat agcggtccgc acggcttctc ggaaattctc tacgccttca cctcgaccaa
      841 cgccaacaac ggctccgcgt tcgccggact cgggccgaac ctgttctaca atctcaccac
      901 cggcgtcaac atgatgttcg ggcgctttac ggtggcgatt ccggcgctcg cgctggcggg
      961 cgcgctggcc ggcaaaaaag ccgtgccgga aagtgccggt accttcacca cccattcgcc
     1021 ggttttcgtc gccctgctga tcggagtgat cgtcatcgtc ggcgcgctga cgttcctgcc
     1081 ggccgacgcg ctcggcccga tcgtcgaaca cctcttcgtc caacaaggaa aaacgttcta
     1141 gtgaaaggtc actaatgctg ccggttcgtc gtttggagcg ccgtcccaag gcccgctcgc
     1201 tgttcgaccg ggcgatcatc gtccgtgcga tcggcgactc gttcaaaaaa ctggatccgc
     1261 gtgggcaggc gcgcaacccc gttatgttcg tggtggaagt cggcgcgctc gtcacctcga
     1321 tattcttcgt gcgcgaccta gcgcggcaca ccggcaccgc cgggttcgat ttatcgattt
     1381 cggcttggct gtggttcacg gtactgttcg cgaatttcgc cgaagccgtg gccgaggggc
     1441 gtggaaaagc gcaagccgat tacctgcggc ggacgaaatc cgataccacc gcacgactgc
     1501 tcggcgagga cggcaaggaa gaagccgttc cgagcacgag cttgcgcaag ggcgatcggt
     1561 tcgtcgttgc ggccggcgag atcgtcgcgg ccgacggcga ggtgctcgag ggcgcggcga
     1621 ccgttgacga gtcggcgatt accggcgaat ccgctccggt cattcgggag tccggcggcg
     1681 atcgcagcgc ggttaccggc gggacgcgcg tgctgtccga ccgcatcgtc gtacgcgtga
     1741 ccgccaatcc cggggagagt tttctcgacc ggatgatcgc gctggtcgaa ggcgcgcagc
     1801 gccagaagac gcccaacgaa atcgcgcttt cgattttgct cgccgggctg acgatcatct
     1861 tcgtgatcgc ggtggctacg ctcgcaccgt attcgatcta tgccggcgcc gcgcaaagcg
     1921 tcacggtctt gatcgctttg ctcgtgtgcc tcattccgac gacgatcggg ggcctgctgt
     1981 cggccatcgg catcgccggg atggaccgcg tcatgcagcg gaacgttttg gcgatgagcg
     2041 gccgtgcggt cgaggcggcc ggcgacgtcg atacgctgct gctcgataag accggtacga
     2101 tcacgctcgg gaatcgccaa gccgtcgaga tcattccggc cgacgggatc gacatgcaag
     2161 acgcggcccg gatggcctat ttgtcgtcga tggccgacga gacgccggag ggacgttcga
     2221 tcgtcgtgct cgcgcaggct caaggctcgc gcgacggcga gccggaaggt tcgacgttcg
     2281 tcccgttcag cgcgtatacg cgcatgagcg gcctcgattt ggccgacggc tcgaagattc
     2341 gcaagggcgc ccccgactcg gtgctggcgt gggtccgcga acaaggcggg aatccgtcgg
     2401 cggagctctc gccgcacatc gaccggatcg cccgcagcgg cggcacgccg ctgctgctgg
     2461 cgcgcaacgc ggtcgtgctc ggcgtcatct acctcaaaga cattctcaag ccgaacatgc
     2521 gcgagcgttt cgaccggatg cgcgcgatgg gcatacggac ggtgatgatc accggcgaca
     2581 acccgctgac cgcggcgacg atcgcgaagg aagccggcgt cgacgatttc ctggcggaag
     2641 cgacgcccga gacgaagatg gccctcatcc ggcgcgagca gacctcggga cggttggtcg
     2701 cgatgaccgg cgacggaacc aacgacgccc cggcgctggc gcaagccgac gtcggcgtcg
     2761 ccatgaactc gggaacgcag gcggccaagg aagccgggaa catggtcgac ctcgactcga
     2821 aaccgacgaa gcagatcgac gtggtcgagg agggcaagca gctcc
//
LOCUS       DASUCJ010000538         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_265588, whole genome shotgun sequence.
ACCESSION   DASUCJ010000538 DASUCJ010000000
VERSION     DASUCJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265588"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFU90_06385"
     CDS             <1..508
                     /locus_tag="VFU90_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU5479441.1"
                     /translation="MTPQEFNQFRDNLNPASGFQSVQFREMEFMCGLRRPDVLKYVEL
                     DDAQRARLERRQNQPSLYDHAKALLARRGFGVDSSEALVESFRRIYSAESEYYDLYLL
                     LEDLIEFDERLLLWRGRHVRMVERMIGQKKGTGGSLGAAYLAKTLDNKLFPELWEVRT
                     LLGGPGYG"
     gene            516..1664
                     /locus_tag="VFU90_06390"
     CDS             516..1664
                     /locus_tag="VFU90_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5479442.1"
                     /translation="MTDAIATQALRREDFPILADSTYLVSHSMGAASLASRRALEEYW
                     SQWAADGPEAWERWLPAIGEIADGIGAIVGAVPGSVFLGPNVSVLQAAVATSIDFSGT
                     RNEVVYEALQFPSLTYVWREWERYGAVTRVVPSGDGRSIPTERIVEAITEKTAIAVLS
                     HAYYVSGAIADVRTIQRHCREVGALLCVDAYQTTGIYPYDVTEWDLDLVTGGSHKWLC
                     GGPGCGWLYVKPSLLERFRPAVTGWMAHARPFAFEPAPIVPAPNMYRFGNGTPTIPGY
                     AVAKPGHDAIRAVGVPRIREHNVRLTTRLAEMALERGLRVNTPLEPSRRTGWIGIDFD
                     GSERVCRALIARRVFVDYRPGCGIRVSPHFYTADDEIGAFFEALDALR"
     gene            1698..3035
                     /locus_tag="VFU90_06395"
     CDS             1698..3035
                     /locus_tag="VFU90_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5479443.1"
                     /translation="MIVDHRNVGAAFRRLSIPSAAAQLGDQVLGVVDTIAIGSIGTVA
                     LAGATAATTVFMTLLFGIFGLMTGTSIIAAQRIGAHDVDGFARTVRAGAIVPFLAALV
                     CFAAACAGSPAVIHALVGNLASAPASANYLVLRCASMLPIVVSGTLIVGLGAAGNRAL
                     GVLVLVVINLIHIPLLLMLGLGWWTHHPMGVTGIGLSSLLSETAGAIYAVAYVARRPI
                     YRIFSGWTVSWRLALSSARLGLPESVFLLGVMLPDVFIVAMLAQLGPIVVSAFRALNV
                     VSDLTFVFPSPLQSAAQTVIGQRLGARDVAGARWFLKRAQSVALVVTAIAGVAAAALA
                     WPLAYVFTLNAAVASLAALPLALHMITLPLKGWSMVSLSPIRASGDTRFSMTVGIACS
                     ALVIPFAWFGIERLHLGLYAVPLGWIAAWSARALLTQWKLREGGWMRAPALAA"
BASE COUNT          433 a         1057 c         1005 g          548 t
ORIGIN      
        1 gatgacgccc caagagttca accagtttcg cgacaatctc aatccggcca gcggatttca
       61 gtcggtgcag ttccgcgaga tggaattcat gtgcggtctt cgccggcccg acgtgctcaa
      121 gtacgtcgag ctcgacgacg cccagcgcgc gcgcctcgaa cgccgccaga accagccgtc
      181 gctgtacgac catgcgaaag cgctcctggc gcggcgcgga ttcggcgtgg actcctcgga
      241 ggcgctggtc gaaagctttc gccggatcta ttccgccgaa agcgaatact acgacctcta
      301 cctgctgctc gaagacctga tcgagttcga cgaacgcttg ctcttgtggc gcggccgcca
      361 cgtccgtatg gtcgagcgca tgatcgggca aaagaaagga accggcggat cgctgggtgc
      421 ggcgtatctc gccaagacgc tcgacaacaa actctttccg gagctgtggg aagtacgcac
      481 cctgctcggc ggccccggat acggctagga gaacgatgac cgacgcgatc gcaacgcagg
      541 ccttacggcg cgaagacttt ccgattctcg ccgattcgac gtatctcgtc agccattcga
      601 tgggagccgc ttcgctcgcg tcgcggcggg cgctcgagga atactggtcg cagtgggcgg
      661 ccgacggccc cgaggcctgg gaacgctggc ttccggccat cggcgaaatc gccgacggga
      721 tcggcgcgat cgtcggtgcg gtgcccggca gcgtattcct ggggccgaac gtgtcggtgc
      781 tccaggcggc ggtggcgacc tcgatcgatt tctccggcac ccgcaacgaa gtcgtttacg
      841 aggccctgca gtttccgtcg ctcacgtacg tatggcgcga gtgggagcgc tacggcgcgg
      901 taacgcgcgt cgtgccgtcc ggcgacggac gctcgatccc gaccgagcgc atcgtcgaag
      961 cgattaccga aaagaccgcg atcgcggtgc tgtcgcacgc gtactacgtg tcgggcgcga
     1021 tcgccgacgt acgaaccatc cagcggcact gccgcgaagt cggcgcgctg ctgtgcgtcg
     1081 acgcgtatca aaccaccggt atctatccgt acgacgtcac cgaatgggat ctcgatctcg
     1141 ttaccggcgg ctcgcacaag tggctgtgcg gcggccccgg ctgcggctgg ctctacgtca
     1201 aaccgtcgct gctcgaacgc ttccgtccgg ccgtcaccgg gtggatggcg cacgcgcgtc
     1261 cgttcgcgtt cgaacccgcg ccgatcgtcc ccgcgcccaa catgtatcgc ttcggcaacg
     1321 gcacgccgac gatcccgggc tatgcggtcg ccaaacccgg ccacgacgca atccgcgccg
     1381 tcggcgtgcc gcgcattcgc gaacacaacg tccggctgac gacgcggctc gccgagatgg
     1441 cgctcgaacg gggcctgcgc gtgaacacgc cgctcgagcc gagccgccgc accggctgga
     1501 tcggcatcga cttcgacggc tccgagcgcg tctgccgcgc gctgatcgcg cgccgcgtct
     1561 tcgtcgatta ccggcccggc tgcggcatcc gcgtgagccc gcatttctac accgccgacg
     1621 acgaaatcgg cgcgttcttc gaggcgctcg acgcgctgcg ctgaaaccgc ggcgtgccgg
     1681 tgcgggtcgc acggtacgtg atcgtcgatc atcgcaacgt aggcgccgca ttccggcgcc
     1741 tttcgattcc gagtgccgcc gcgcagctcg gcgatcaggt gctgggcgtc gtcgacacga
     1801 tcgcgatcgg cagcatcggc acggtcgcgc tggccggcgc gaccgccgcc accaccgtct
     1861 tcatgacgct gctgttcggc atcttcggcc tgatgacggg cacgtcgatc attgcggcgc
     1921 agcgcatcgg cgcgcacgac gtcgacggtt tcgcgcgaac ggtgcgcgcc ggcgcgatcg
     1981 ttccgttcct ggccgcgctc gtctgctttg cggccgcctg cgccggaagt ccggccgtga
     2041 tccacgcgct tgtcggcaac ctcgcgagcg ccccggccag cgcgaactac ctggtgctgc
     2101 gctgcgcgtc gatgcttccg atcgtcgtct cgggaacgct gatcgtcggg ctcggtgcgg
     2161 ccggcaatcg cgctctcggc gtgctggtgc tggtcgtcat caacctcatt cacattccgt
     2221 tgctgctgat gctcgggctc ggctggtgga cgcatcaccc gatgggcgtc acggggatcg
     2281 ggctctcgtc gctactgagc gaaacggccg gcgcgatcta cgccgtcgcc tacgtcgcgc
     2341 gccggccgat ctatcggatc ttctccggct ggaccgtttc gtggcgcctg gcactgtcga
     2401 gcgcgcgttt gggactgccg gaatcggtct tcctgctcgg cgtcatgctg cccgacgttt
     2461 tcatcgtggc gatgctcgcg cagctcggcc cgatcgtcgt ctcggcattt cgcgcgctca
     2521 acgtcgtctc cgatctcacg ttcgtcttcc cgagcccgct gcagagcgcg gcgcagacgg
     2581 tgatcgggca gcgtctcggt gcgcgcgacg tggccggagc gcgctggttc ctcaagcgcg
     2641 cgcagagcgt cgcgctggtc gtcaccgcga tcgccggggt cgcggccgcc gcgctcgcct
     2701 ggccgctggc gtacgtcttc accctgaacg ccgcggtcgc aagcttggcg gcgctgcccc
     2761 tcgcgctgca catgatcacg ctgccgctca aaggctggtc gatggtgtcg ctctcgccga
     2821 tccgcgcctc cggcgatacg cggttttcga tgaccgtcgg aatcgcgtgc agcgcgctgg
     2881 tgattccgtt tgcgtggttc ggcatcgaac ggctgcatct cgggctctac gccgtcccgc
     2941 tcggctggat cgccgcctgg agcgcgcgcg cgctgctcac gcagtggaag ctgcgcgaag
     3001 gcggctggat gcgcgccccc gccctcgccg cgtaatcgct cgt
//
LOCUS       DASUCJ010000539         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_402767, whole genome shotgun sequence.
ACCESSION   DASUCJ010000539 DASUCJ010000000
VERSION     DASUCJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402767"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1045
                     /locus_tag="VFU90_06400"
     CDS             53..1045
                     /locus_tag="VFU90_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479444.1"
                     /translation="MSTLRSVCLRACSLLAVALLSACAGGSMQPPPPGGFAPAARRAG
                     MQTLQPLRSWVNPEAKTQKLLYISDLTDQVVSMYSYPGGKLEGQITGFFNPEGLCVDH
                     VGNVWVTNDTSDGVHQITEYAHGGTTPIANLLDPDGRTNGCSINPVNGDLAVTNFFGA
                     TGQGSVSIYKKAQGSPIEYIDLDLYYYYYCGYDAKGDLFVDGWSSGSNFAFAELPRGG
                     KKLKTVVLNHSITYPGGVQWDGKHVAVGDQYGPIYQFDIQGNVGTEVGSTPLNGEKQI
                     VQFWIQGRYVVGPNEYGANVMLWNYPAGGSVVKTLSGPGQGATVSRVPPSAPPV"
BASE COUNT          248 a          425 c          370 g          210 t
ORIGIN      
        1 gatttcgaaa tgctatgctg aatgcgaaac cgccagcgcc gaggagggaa ccgtgagcac
       61 tttgcgttcc gtctgtctca gagcgtgttc gctgcttgcc gtcgcgctgc tgagcgcctg
      121 cgccggcggc tcgatgcagc ccccgccgcc cggcggattc gcgcccgcgg cgcgtcgcgc
      181 cggcatgcaa acgctccagc cgctccgctc gtgggtaaac cccgaagcca agactcagaa
      241 gctgctctac atctccgacc tcaccgatca ggtcgtctcg atgtattcct atccgggcgg
      301 caagttggaa gggcaaatca ccggcttctt caatcccgag ggcttgtgcg tcgaccacgt
      361 cggcaacgtg tgggtcacca acgacacgtc cgacggcgtg catcagatca ccgaatacgc
      421 ccacggcggc acgacgccga tcgccaatct gctcgatccc gacggacgca ccaacggctg
      481 ttcgatcaat cccgtcaacg gcgatttggc cgtcaccaac ttcttcggtg cgaccggtca
      541 aggcagcgtt tcgatctaca aaaaagccca gggcagcccc atcgaataca tcgacctcga
      601 cctctactat tactactact gcggctacga tgccaaaggc gatctcttcg tcgacggctg
      661 gagcagcggc agcaacttcg cgttcgccga gctgccgcgc ggcggaaaga agctgaaaac
      721 ggtcgtgctc aatcacagca tcacgtatcc cggcggggtc cagtgggacg gcaagcacgt
      781 cgccgtcggc gaccagtacg gcccgatcta tcagttcgac attcaaggca acgtcggcac
      841 cgaagtcggc tcgacgccgc tcaacggcga aaaacaaatc gtgcagttct ggattcaggg
      901 acgctacgtc gtcggcccca acgagtacgg cgccaacgtc atgctgtgga actacccggc
      961 cggcggttcg gtggtcaaga cgctcagcgg ccccggccag ggcgcgaccg tcagccgcgt
     1021 tccaccgtcg gcgccgccag tttagcgccg acagcccgca gcggtgtcat cctgagcgga
     1081 ggcgcgaagt gtgtgtcatc ctgagcggag gcgcgaagcg ccgcagtcga aggacgcact
     1141 tgcatctgca cacgctgttc gccgaatacg cacccgtcca gcccgtcgaa atttacggcg
     1201 tccccggcgc caccgccggc agaatgtact tcggaacgaa cacgcgctgc ggc
//
LOCUS       DASUCJ010000540         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1396532, whole genome shotgun sequence.
ACCESSION   DASUCJ010000540 DASUCJ010000000
VERSION     DASUCJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396532"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..502
                     /locus_tag="VFU90_06405"
     CDS             101..502
                     /locus_tag="VFU90_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479445.1"
                     /translation="MFSTSRRHVALLALLTAASLPAAAVAQQHCSQEVLKVRGTPVTI
                     GYCVTGNPRNDGAGEVVVPVAATYSAPAASFGQQRDLRFIAGERNSRILESLNLDRLG
                     MTGVLHLTLVYTGEVVRVEGALLTPGAITIK"
     gene            640..>1942
                     /locus_tag="VFU90_06410"
     CDS             640..>1942
                     /locus_tag="VFU90_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479446.1"
                     /translation="MPCTAVKPDLTVRERAHHLYQAIPPGLFRVDDANESVAWRVSPE
                     PFALSAAQVDEIERLGHDLLVFYRALNDLYNRSARGTAPAFVAEYLDRGKPEGIVKLA
                     RQNRFKQELPGVIRPDLMLTEDGFVATELDSVPGGMGFLGAMTQAYCQLGIESLGGDD
                     GIPKGFGDMLRHSTGVDRPTVAVVVSEESADYRNEMGWLCGELRRLGTVNAWLRKPQD
                     VFFTEEGLFIRHEDGREERLDAIYRNFELFDLLNVPKQELMLYAARHNRVKMTPPPKA
                     DLEEKLAFALFHHYALAPLWRAALGAERFERLRAIFPKTWIMDPRPLPPQAIVAGLEA
                     GGAPVADFRSLIDLGKSERDYVVKPSGFSALAWGSRGVKIANDLTKEEWSATVDAALA
                     AFDTTPYVLQRFHKGKRLRQTYFDRESDDVRTYDGRARLCPY"
BASE COUNT          357 a          662 c          601 g          322 t
ORIGIN      
        1 atcgcgggcg gcggcaccac gtcaactata acccgcgcgt caatcgaaac ggggcgaacg
       61 accggcgggt gcgacctttg acggaggcga atggggcggc gtgttctcaa cctcccggcg
      121 gcacgtcgcg ctgctcgcgc tgctgaccgc tgcctcgctc cccgccgcag ccgtcgcgca
      181 gcagcattgc agccaagagg tgctcaaggt tcgcggtacg ccggtcacca tcgggtactg
      241 cgttaccggc aatccgcgca acgacggggc gggcgaagtc gtcgtaccgg tggcggcgac
      301 ctactcggca ccggcggcga gcttcgggca gcagcgcgac ctgcgcttca tcgccggcga
      361 gcgaaactcg cgaatcctag aaagcctgaa tctcgaccgg ctcggcatga ccggggtact
      421 ccatcttacg ctggtgtaca ccggcgaggt cgtgcgcgtt gagggggcgc tgctgacccc
      481 cggggcgatc acgatcaagt aggccgcgcc ggcgaaactg caaccatctc ctgcggacgg
      541 cactttaaaa actaacagaa aaccatgcgg cgcctgtaca tcgctttcag cagctcgaag
      601 atacccgacg gttgtcgata gggagaccgc ctctttacca tgccctgtac ggctgttaaa
      661 cccgatctga cggttcggga gcgagcgcac cacctctacc aggccattcc acccggactt
      721 ttccgggtcg acgacgccaa cgaaagcgtc gcctggcgcg tgagccccga accgtttgcg
      781 ttatccgccg cccaggtcga cgaaatcgaa cggctcggcc acgacctcct cgtcttctat
      841 cgcgcgctca acgatctcta caatcgcagc gcgcgcggca ccgcgccggc cttcgtcgcc
      901 gagtacctcg atcgcggcaa gcccgaaggc atcgtgaagc tcgcgcggca aaaccgtttc
      961 aagcaggagc ttcccggcgt catccggccg gatttgatgc tgaccgagga cggcttcgtc
     1021 gccaccgagc tcgacagcgt ccccggcggc atgggattcc tcggtgcgat gacccaggcg
     1081 tactgccagc tcggcatcga gagcttgggc ggcgacgacg gcatccccaa aggcttcggc
     1141 gatatgctgc gtcactctac cggcgtcgat cgcccgaccg tggccgtcgt ggtttccgaa
     1201 gagtccgccg actaccgcaa cgagatgggg tggctgtgcg gcgagctgcg ccggctcggc
     1261 accgtcaacg cgtggctgcg caaaccccaa gacgtcttct tcaccgaaga gggactcttc
     1321 atccgccacg aagacggacg cgaggaacgt ctcgacgcga tctaccggaa tttcgagctg
     1381 ttcgatttgc tcaacgtccc caagcaggag ctgatgctct atgcggcgcg gcacaaccgc
     1441 gtgaagatga ccccgccgcc gaaagccgat ctcgaggaga agctcgcgtt cgcgctgttc
     1501 catcactacg cgctcgcgcc cctatggcgc gccgcgctcg gtgccgagcg cttcgagcgc
     1561 ttgcgcgcaa tctttcccaa aacctggatc atggatccgc gaccgctccc tccacaggcg
     1621 atcgtcgccg gactggaagc cggcggcgct ccggtagcgg actttcgcag cctgatcgac
     1681 ttgggaaaga gcgagcgcga ctacgtcgtc aagccctcgg gtttctcggc actggcctgg
     1741 ggatcgcgcg gggtaaagat cgccaacgat ctcaccaagg aagaatggag cgccaccgtc
     1801 gacgccgcgc tcgcggcgtt cgacaccacg ccgtacgtgc tccagcgatt tcataagggc
     1861 aaacggctgc gccagaccta tttcgaccgc gagagcgacg atgtccggac gtacgacggg
     1921 cgcgcgcgcc tctgtcccta ct
//
LOCUS       DASUCJ010000541         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_723853, whole genome shotgun sequence.
ACCESSION   DASUCJ010000541 DASUCJ010000000
VERSION     DASUCJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723853"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFU90_06415"
     CDS             complement(<1..208)
                     /locus_tag="VFU90_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5479447.1"
                     /translation="MKTTSSKDGPRTDSRPFNLQLRLPPLPRSARTGRDALAALAGVH
                     DVAAADIENLTFALGEALANAIEHA"
     gene            235..1077
                     /locus_tag="VFU90_06420"
     CDS             235..1077
                     /locus_tag="VFU90_06420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5479448.1"
                     /translation="MHRVKPAGEGAAVYVLRCADGSLYTGATTDLARRLATHRRGTAA
                     KYTRSRLPVSLIAWWNPPAYDLARSHEARFKRMTRAAKTALLAADEAFGYPLRKAPST
                     APPIEEEDRRFMPYTPPQPDELESLKEEARTSRVRIATDKGDIVVTLFPDDAPVHSAA
                     FLKLAKAGFYDGLAFHRVEPGFVIQGGDPDGDGTGGPGYRLHAEFNARPHLRGTLAMA
                     RASDPNSAGSQFYLCLDDARFLDGQYTVFGTMTDGFEALDQIRRGDKMRKLTIEPNGA
                     PASG"
     gene            complement(1069..1935)
                     /locus_tag="VFU90_06425"
     CDS             complement(1069..1935)
                     /locus_tag="VFU90_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5479449.1"
                     /translation="MNRAAFLAGAAAVAAVRPAPASAATLYGRVAAAAQSLPGEIGVY
                     ARTLAPGGPLVTYQAHEVFPTASVIKVLIMATAFAAQERRPGALSETIVFDRGADLIG
                     GSDFMSEQDDGARFTVKQLIVPMIQVSDNTAANLLIEHFGTNAINAMGKRAGMTRTHL
                     GRTFLDTSAVVHHHDNVSTPADMGRLLYAIERGAHEAIPTIVSAEHCRSMVDVMLGQT
                     DREKIPQGLPPGTKVANKTGEITGTRNDVAIIGPFGDQPVILTIMTKDLTDYWAANAA
                     IHAIARAVYASA"
     gene            complement(1932..>2206)
                     /locus_tag="VFU90_06430"
     CDS             complement(1932..>2206)
                     /locus_tag="VFU90_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5479450.1"
                     /translation="LRWVVMSLEQDFTRYPISIYSRGVRIVLSFVLPFAFMNYFPATY
                     FLQKTDTGKHLNPAVGLLTPAIGIAWACVAYAFWRIGLRHYQGTGS"
BASE COUNT          382 a          704 c          753 g          367 t
ORIGIN      
        1 cggcgtgttc gatcgcgttg gccagcgcct cacccagggc gaaggtgaga ttttcgatgt
       61 cggcggcggc gacgtcgtga acgccggcca acgcggccag cgcgtcgcga ccggtacggg
      121 ccgaacgcgg caggggaggg aggcgtaact gcaggttgaa ggggcgcgag tcggtgcggg
      181 gcccgtcctt actcgaggtc gttttcatcg gaaccgttcg agattctacc gcaagtgcac
      241 cgggttaaac cggccggcga aggcgccgcg gtttacgtgt tgcgctgcgc cgacgggtcg
      301 ctctacacgg gggcgacgac cgacctggcg cggcggctgg cgacccaccg gcgcggcacg
      361 gcagccaagt atacgcgtag ccggttgccg gtcagcttga tcgcgtggtg gaacccgccc
      421 gcgtacgatc tcgcccgttc gcacgaagcc cggttcaagc ggatgacgcg cgccgccaaa
      481 acggcgctgc tggcggcgga cgaggctttc ggctatccgt tgcgcaaggc cccgagcacc
      541 gcaccgccga tcgaagaaga ggaccgccgt ttcatgccgt acacgccgcc gcagcccgac
      601 gaactcgaga gcttgaagga agaagcgcgc acctcgcgcg tacgcatcgc caccgacaag
      661 ggcgatatcg tcgtcacgct cttcccggac gacgcgccgg tgcattcggc cgcctttctg
      721 aagctggcga aagccgggtt ttacgatggg ctggcgtttc atcgcgtcga gccgggtttc
      781 gtaattcagg gcggcgatcc ggacggcgac gggaccggcg gcccgggtta ccggctgcac
      841 gccgagttca acgcgcgccc gcacctgcgc ggaacgctgg cgatggcccg cgcgtcggat
      901 ccgaacagcg cgggttcgca gttctacctc tgcctcgacg acgcgcgctt tctcgacggc
      961 cagtataccg tcttcgggac gatgaccgac ggtttcgagg cgctcgatca gatccggcgc
     1021 ggcgacaaga tgcgcaagct gacgatcgaa ccgaacggcg cgccggcgtc aggctgaggc
     1081 gtacaccgca cgggcgatcg cgtgaatggc ggcgttggcg gcccaatagt ccgtgagatc
     1141 tttcgtcatg atcgtgagga tcaccggctg atcgccgaag ggaccgatga tcgcgacgtc
     1201 gttacgcgtt ccggtgatct cgccggtctt attggcgacc ttcgtccccg gcggcaaccc
     1261 ttgcggaatc ttctcgcgat cggtttgtcc gagcatgacg tcgaccatcg aacggcagtg
     1321 ttcggccgaa acgatcgtcg gaatcgcttc gtgcgcgccg cgctcgatgg cgtacaatag
     1381 ccgccccatg tcggcgggcg tcgagacgtt gtcgtgatgg tgcacgaccg ccgacgtgtc
     1441 gagaaacgtc cgccccagat gcgtgcgcgt catcccggcg cgtttgccca tagcgttgat
     1501 cgcgttggtt ccgaagtgtt cgatgaggag gttagccgcg gtgttatcgc tgacttgaat
     1561 catcggaacg atcagctgtt tcaccgtaaa gcgcgcgccg tcgtcctgtt ccgacatgaa
     1621 atcggatcct ccgatcaagt cggcgccgcg atcgaagacg atcgtttcgc tcagcgcccc
     1681 cggccggcgc tcctgcgccg caaaggcggt ggccatgatc agaaccttga tgaccgacgc
     1741 ggtggggaag acttcgtggg cttgataggt taccagcgga ccgcccggcg cgagcgtgcg
     1801 agcgtagacg ccgatctctc ccggcaagct ctgcgcggcc gccgcgaccc tgccgtagag
     1861 cgtcgcggcg gaagccggtg ccgggcgcac cgccgcgacc gccgcggcgc cggcgaggaa
     1921 cgcggcgcgg ttcacgaacc ggtcccctga tagtgacgta gtccgatccg ccagaacgcg
     1981 tacgccacgc acgcccaagc gatgccgatg gccggcgtca gcaacccgac ggccgggttc
     2041 aggtgcttcc cggtatcggt tttttgcagg aaatacgtcg ccgggaagta gttcatgaag
     2101 gcaaacggca ggacgaagga caggacgatg cgcacgcccc gcgagtagat gctgatggga
     2161 taacgcgtaa aatcctgctc gagcgacatg acgacccagc gcagcg
//
LOCUS       DASUCJ010000542         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_372771, whole genome shotgun sequence.
ACCESSION   DASUCJ010000542 DASUCJ010000000
VERSION     DASUCJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372771"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..>1114
                     /locus_tag="VFU90_06435"
     CDS             57..>1114
                     /locus_tag="VFU90_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479451.1"
                     /translation="MTFAAPRRAIGIAALALGAVASVAFAEEPIRIGSFLSVTGPAAF
                     LGDPEQKTLQMYVERINAAGGILGRPIVLTVYDDGGDADKARTFAKRLLDEDKVDVIV
                     GGSTTGTTMAVIPLVEQAQVPFVSLAGAVSIVEPVKKWVFKTPHTDRMACERIFGDMR
                     ARKLVKVALISGTGGFDKSMRGECLKVAPKYGIEVVADETYGAGDTDMTAQLARIKAT
                     PGVQAVLNAGFGQGPAIVTKNYRQIGLTLPLYQSHGVASKEYIKLSGDAAEGVRLPAA
                     ALLVAEILPESDPQRAVVVAYKHDYESRYKTDVSTFGGHAYDGLMLVVDAVKRAGGTD
                     HAKIREALESTHGYIGTGG"
BASE COUNT          202 a          352 c          391 g          169 t
ORIGIN      
        1 attctagccg cttgcggcga cgcgcacgat agctgtcgag gctggaggag attgccatga
       61 cgttcgcagc accacgcaga gccatcggta tcgccgcgct tgcgctgggc gcggtggcat
      121 ccgtcgcgtt cgccgaggag ccgatccgca tcggctcgtt cctctcggtg accgggccgg
      181 cggcatttct gggcgacccc gagcagaaga cgctccagat gtacgtcgag cgaatcaacg
      241 ccgcaggcgg aattctcggt cgcccgatcg tgctcacggt ctacgacgac ggcggcgacg
      301 cggacaaggc acgcacgttc gcgaagcggc tgctcgacga ggacaaggtc gacgtcatcg
      361 tcggcggctc gaccaccggc accacgatgg cggtgattcc gctcgtcgag caggcgcagg
      421 tgccgttcgt gtcgctcgcc ggtgcggtca gcatcgtcga gccggtgaag aaatgggtgt
      481 tcaagacgcc gcacaccgac cgcatggcgt gcgagcgcat cttcggtgac atgcgtgcgc
      541 gcaagctcgt caaagtcgcg ctgatctccg gcaccggcgg cttcgacaag tcgatgcgcg
      601 gcgagtgcct caaggtcgct ccgaaatacg gcatcgaagt cgtggcagac gagacctacg
      661 gcgccgggga caccgacatg accgcacagc tcgcgaggat caaggcgacg ccgggtgtgc
      721 aggcagtgct caacgccggc ttcggtcagg ggccggcgat cgtgacgaag aactaccggc
      781 agatcgggct gacgctgccg ctgtaccagt cgcacggcgt ggcgtcgaag gaatacatca
      841 agctctccgg cgatgcggcg gaaggcgtgc ggctgcctgc ggcggcgctg ctcgtcgcgg
      901 agattctgcc cgagagcgat ccgcaacgcg cggtggtcgt cgcctacaag cacgattacg
      961 agagccgcta caagaccgac gtgtcgacgt tcggcggcca tgcgtacgac ggtctgatgc
     1021 tggtggtcga cgcggtgaag cgcgccggcg gcaccgatca cgcgaagatc cgcgaagcgc
     1081 tcgagagcac gcacggctac atcggcaccg gagg
//
LOCUS       DASUCJ010000543         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_265630, whole genome shotgun sequence.
ACCESSION   DASUCJ010000543 DASUCJ010000000
VERSION     DASUCJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265630"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFU90_06440"
     CDS             <1..216
                     /locus_tag="VFU90_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479452.1"
                     /translation="NGPHVRLGTYAWVAGLAASLPFWNQQWYTGPFAAAFPQFGDLSY
                     YVGFAVAAIVMTAAKRETSRVAASSTP"
     gene            216..1115
                     /locus_tag="VFU90_06445"
     CDS             216..1115
                     /locus_tag="VFU90_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5479453.1"
                     /translation="MLRVDRVRKEFGSVQAVRDVTFTIARGSTFGLLGPNGAGKTTTM
                     RMIVGIYPVDGGSITWNNSPIDDRMRRRFGYLPEERGLYGKMKVREQIVYFGRLHGLH
                     DPEISQRADSWLEQLGLTEYANRPCAELSKGNQQKVQVACAAVHDPELLILDEPFSGL
                     DPVNAEILLKTLTELKKRGTTLVLSSHQMWQLEQICDEFCIITGGVNRASGTLEQLRA
                     AWPTRVIKVEPPSDALRSIMERVPSARALEGNGALYYEVPVGTPYPALLRDLVAADAV
                     TRFDALEPSLQEIYLHTIEARTP"
     gene            1112..>1345
                     /locus_tag="VFU90_06450"
     CDS             1112..>1345
                     /locus_tag="VFU90_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479454.1"
                     /translation="MNEFVTVYSAEIMRRLQSRTFWIGLVFGIIGIAAMMELPRFFAS
                     YTIQSKHVLIAGDPKLVASARPLLDKDFTIVGER"
BASE COUNT          250 a          423 c          418 g          254 t
ORIGIN      
        1 aacggcccgc acgtacggct cggaacgtac gcatgggtag cgggcctcgc ggcgtcgctg
       61 ccgttttgga accagcagtg gtacaccgga ccgttcgccg cagcgtttcc gcagttcggc
      121 gatctttcat attacgtagg ctttgcagtc gccgcgatcg tgatgaccgc cgcaaagcgg
      181 gaaacttcgc gcgtggcggc aagtagtacg ccctagtgct tagagtcgat cgcgtccgca
      241 aagagttcgg ttcggttcaa gccgttcgcg atgtaacctt taccatcgcg cgcggttcga
      301 ctttcggtct cttgggcccc aacggcgccg gcaagaccac gacgatgcgc atgatcgtcg
      361 gcatctatcc ggtcgacggc ggcagcatta cctggaataa tagcccgatc gacgatcgga
      421 tgcgccggcg cttcggctat ttgcccgagg agcgcggact gtacggcaag atgaaagtcc
      481 gcgagcaaat cgtgtacttc ggacgcctgc acggcttgca cgatccggag atttcgcagc
      541 gcgcggattc gtggctcgag cagctcggct tgaccgaata tgcgaatcgc ccgtgcgccg
      601 agctctcgaa gggcaatcag cagaaagtcc aggtcgcgtg cgcggcggtg cacgatccgg
      661 aactgctcat cctcgacgag ccgttctccg gactggaccc ggtcaacgcg gagattttgc
      721 tcaagacgct gacggagctg aaaaagcgcg gcacgacgct cgtgctttcg agccaccaga
      781 tgtggcagct cgaacagatc tgcgacgagt tctgcatcat taccggcggc gtcaaccgcg
      841 cctcgggaac gctcgaacag ctgcgcgccg cgtggccgac gcgcgtcatc aaagtcgagc
      901 cgccttcgga tgcgcttcgg agcatcatgg aacgcgtccc aagcgcccgt gccctcgagg
      961 gcaacggcgc gctctactac gaggtaccgg tgggaacgcc gtaccccgcg ctgcttcgcg
     1021 acctggtcgc cgccgacgcc gtcacgcgct tcgacgcact cgagccgtcg ctgcaagaga
     1081 tctacctgca cacgattgag gcacgcacgc cgtgaacgaa tttgttaccg tttattccgc
     1141 agagattatg aggcggctgc agtcgcgcac gttctggatc gggctggtct tcggcatcat
     1201 cggcatcgcc gcgatgatgg aactgccgcg gtttttcgca tcgtacacga ttcagagcaa
     1261 gcacgtgctg atcgcaggcg atccgaagct cgtcgcttcg gcgcgcccgc tgctcgacaa
     1321 ggatttcacc atcgtcggcg agcgc
//
LOCUS       DASUCJ010000544         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1518929, whole genome shotgun sequence.
ACCESSION   DASUCJ010000544 DASUCJ010000000
VERSION     DASUCJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518929"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFU90_06455"
     CDS             complement(<1..849)
                     /locus_tag="VFU90_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5479455.1"
                     /translation="MPTQILFATNRARTDNGAGMPQFGSTAITNPDNLWCGVATVDGV
                     SDDDPATGTISAIANLNQGWFAQAQIDALTQSTNDLLVFVHGTDNDFVDAVTRCAYNQ
                     TWLSNGAGRGIDVIVFSWPSLAYGDLIDIFQDHTDYETDQKTASASAPHFGLFLQRLL
                     ALKPLLGGRTVSILCHSMGNYMLGAAVEAWFAQPQTAPPLFAQAILAAADEPSTAFLG
                     SKGKRLSKLRQLAAGITVYFNDNDILMALSDIVNPFTPLGLAGPTGASNTHIYPTDAY
                     QFVDCTN"
     gene            complement(877..>1066)
                     /locus_tag="VFU90_06460"
     CDS             complement(877..>1066)
                     /locus_tag="VFU90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479456.1"
                     /translation="ERVSVAEGVESAAAAALLAQRLAIDMPITATVNAILHRGAAIDD
                     AIAALLSRPRKSEGHATS"
BASE COUNT          182 a          315 c          366 g          203 t
ORIGIN      
        1 gttggtgcag tcgacgaact gataggcatc ggtcggatag atgtgcgtgt tcgacgcgcc
       61 ggtcggaccg gcgaggccga gcggcgtgaa cggattcacg atgtcgctca gcgccatcag
      121 aatatcgttg tcgttgaaat agaccgtgat gccggcggcg agctgacgaa gcttcgacag
      181 gcgcttgccc ttgctgccga ggaacgcggt gctcggctcg tcggcggcgg cgaggatcgc
      241 ctgcgcgaac agcggcggcg cggtttgcgg ttgcgcgaac cacgcctcga ccgccgcgcc
      301 gagcatgtag ttgcccatgc tatgacaaag gatcgatacg gttcgaccgc cgagcagcgg
      361 cttcagcgcc agaagccgct gcaggaacag gccgaaatgg ggcgcggacg cgctcgccgt
      421 cttctggtcc gtctcgtagt cggtgtggtc ctgaaaaatg tcgatgaggt cgccatacgc
      481 caggctgggc cagctaaaaa cgatgacgtc gatcccgcgg ccggccccgt tgctcagcca
      541 ggtctgattg taggcgcagc gcgtgacggc atcgacaaag tcgttgtcgg tgccgtgcac
      601 gaaaaccagg agatcgtttg tcgattgcgt cagggcgtcg atctgcgctt gcgcgaacca
      661 gccctgattc agattggcga tggcggaaat ggttccggtt gccggatcgt cgtcgctgac
      721 gccgtcgacg gtcgcgacgc cgcaccataa attatcgggg ttggttatcg ccgtactgcc
      781 gaattgcggc attccggctc cgttgtccgt tctggctcga ttggtggcga acagaatctg
      841 ggtcggcatc cattccccct ttcgtccggc ggcgatttac gacgtggcgt ggccctcgct
      901 tttgcgcgga cgggacagca gcgccgcgat ggcgtcgtcg atggcggcac cgcgatgcag
      961 gatcgcgttg accgtcgcgg taatcggcat atcgatcgcg agacgctgcg ccagcaacgc
     1021 ggcggcagca gcggattcca ccccttcggc gacggagacg cgctcg
//
LOCUS       DASUCJ010000545         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1457698, whole genome shotgun sequence.
ACCESSION   DASUCJ010000545 DASUCJ010000000
VERSION     DASUCJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457698"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1315
                     /locus_tag="VFU90_06465"
     CDS             200..1315
                     /locus_tag="VFU90_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5479457.1"
                     /translation="MRSLDGNSVITRRRLLQRAAGIWASCGVLSFVSAERTEAGLTSV
                     KDTTSDSVSSGKIALEEHFVIPETLAASYGALGSAEFQRRLADIGSARIAEMDRGGLD
                     VCILSLVGDGIQAIPSIPEAIRVARRANDHLAEQIAKNPKRFKGFAALPLQDPQVAAQ
                     ELTRCIKELGFCGALVNGYSQIDTAESTVFYDLPQYRDFWATVQELDVPFYLHPRSAS
                     AGEPAYEGHRWLISSVWGFAAETSVHALRLMGSGLFDDYPRLKIILGHLGEGLPCNIW
                     RIDNRVSRTLGAKPSAKRPIGHYLRENFYITTSGNFRTPTLIEVVAEVGADRVLYSVD
                     FPFEDTAIAADWFDHAAISDSDREKIGRGNAQRLFHI"
     gene            complement(1360..2349)
                     /locus_tag="VFU90_06470"
     CDS             complement(1360..2349)
                     /locus_tag="VFU90_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5479458.1"
                     /translation="MLRLPRFQYVSPPTVMDALSARFELGSDAMYVAGGTDLYPNMKR
                     RQQTPRHVISLAGIEPLREIHSGPELGLRIGSCVTLAQLAADPIVRRQYPAVAAAAGL
                     VSTPPLRNMGTIGGNLLLDTRCNYYDQTYEWRKAIDFCMKKDGRICWVAPSSPRCWAV
                     QSSDGAPVAVALGASVMLASRGGVRRVAAEDLYHNDGIQYLTKRPEELLVWYVLPPIG
                     AWRASYVKLRRRGSFDFPVLGVAARVDFFPDGTVRDARIVLGAVASYPMRIAAAREAL
                     VGRPLDAASIADAADASFKPAKPMDNTDFTLSWRKEMVRVYVTKALEEIASQS"
     gene            complement(2349..>2786)
                     /locus_tag="VFU90_06475"
     CDS             complement(2349..>2786)
                     /locus_tag="VFU90_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479459.1"
                     /translation="MEEMTYRDAERRLVHHHPSMLEYKSPTTFEMCDVESYLIEAAEP
                     GGPFGAKEVGQGPWLPIPPAVANAVYDAVGVRIDEVPCAPHAVLRALRDKTRGKEPRH
                     GPSSFPDYAFPEPTLIRTPAQGGDGREADHRPHSEVGGPGRAG"
BASE COUNT          507 a          884 c          877 g          518 t
ORIGIN      
        1 ccaaagatat gtcgtagata tatcggcaga ctaagatatg tcgtccgata tatcttcgca
       61 ataccctcgc gaaggtttcg ccgagctcat ctataagatg aggtcgctgt tggggatcat
      121 cccgttcaca catcccgttg cgcgaccgtt tgtcgtagag ttgagagcct ttttcgaccc
      181 agcgaacggg agggcgcgaa tgaggtccct cgacggcaac agcgtgatta cacgacgtcg
      241 gctgctccag cgcgccgccg gaatctgggc atcgtgcggc gtattgtcat tcgtcagtgc
      301 cgaaagaacc gaggccggcc taacgagtgt aaaggacaca acgtccgact cggtctcgtc
      361 cggcaagatt gcgttggaag agcactttgt catccctgag actctagccg cgagctatgg
      421 ggcgctgggt agcgccgaat ttcaaaggcg gttggccgat attggaagtg cgcgaatcgc
      481 cgagatggac cgaggcggtt tggacgtttg cattctttcg ctagtcgggg acggcatcca
      541 ggcaattccg agcattcccg aggcgattcg cgtggcgcgg cgcgcgaacg accatctcgc
      601 cgagcagatc gccaagaatc cgaaacgttt caagggattt gctgcgctac cgctgcagga
      661 tccgcaggtt gcggctcagg agctaacgcg ctgcatcaaa gaactgggat tctgtggggc
      721 gctcgtcaac ggatactcgc agatcgacac ggcggaatcc accgtgttct acgacctgcc
      781 gcagtaccgt gatttctggg ccacagtgca ggagcttgat gttccatttt atctgcaccc
      841 gcgatcggca tccgccggcg aaccggctta cgaaggccat cgttggttaa tcagctcagt
      901 atggggattc gcggcggaga cttcggtcca tgccctccgc ctcatgggca gcggattgtt
      961 cgacgactat ccgaggctca agattattct cggtcacctg ggagaagggc tcccctgcaa
     1021 catctggcgt atcgataacc gcgtatcgcg aacattggga gccaagccga gcgccaagcg
     1081 tcccattggt cattatttgc gagagaactt ttacatcacg acgagcggca attttaggac
     1141 gccgacgctg atcgaagtcg tggccgaagt cggcgccgac cgcgtcctct actcggtgga
     1201 tttcccattc gaagacacgg ccatcgcggc agactggttc gatcacgccg cgatcagcga
     1261 ttcggaccgt gaaaaaattg ggcgtggcaa cgcacagcgg ctatttcaca tttaggtcgg
     1321 cacgttcgac gcgaatgcgc cgtgcactga aatggtcgac tacgactgcg acgctatttc
     1381 ctccagcgcc ttcgtcacgt acacccggac catctctttg cgccacgaga gcgtgaagtc
     1441 ggtgttgtcc atcggttttg ccggcttgaa cgacgcgtcg gcggcgtcgg cgatcgatgc
     1501 ggcatcgagc ggccgcccaa cgagcgcttc ccgcgccgcc gcgatgcgca tcggatacga
     1561 cgcgacggcg ccgagcacga tgcgcgcgtc gcggacggtg ccgtcgggaa agaagtcgac
     1621 gcgcgcggcg acgcctaaca ccggaaagtc gaacgagccg cggcgcctga gcttcacgta
     1681 cgatgcccgc catgcaccga tcggcggcag cacgtaccac acgagcagct cctccggccg
     1741 cttggtgaga tactggatgc catcgttgtg gtagaggtct tccgccgcga cccggcgcac
     1801 gccgccgcgg ctggccagca tcaccgatgc gcctaacgca acggcgacgg gcgctccgtc
     1861 cgacgattgc accgcccaac agcgcgggct cgacggtgcg acccagcaga tccgcccatc
     1921 cttcttcatg cagaagtcga tcgccttgcg ccactcgtac gtctggtcgt aatagttgca
     1981 gcgcgtgtcg agcagcaggt tgccgccgat ggtgcccatg ttgcgcaacg gtggcgtcga
     2041 cacgagcccg gccgcggcgg cgaccgccgg atactgccgt cgcacgatcg gatccgccgc
     2101 cagctgcgcc aacgtgacgc aggagccgat gcgcaggccc agctcggggc cgctgtggat
     2161 ttcccggagc ggctcgatgc cagcgagcga gatgacgtgc cgcggcgtct gctggcgccg
     2221 cttcatgttc gggtacaggt cggtgccgcc ggccacgtac atcgcatcgc tgcctaactc
     2281 gaatcgcgca ctgagcgcat ccatcaccgt tggcggcgac acgtactgga atcgcggaag
     2341 gcgcagcatc agcctgcccg gcccggtccg ccgacctcgc tgtgcggccg atggtccgcc
     2401 tcgcgaccgt cgccgccctg cgcgggcgtc cggatgagcg tcggttcggg gaacgcgtag
     2461 tccgggaacg acgaaggtcc gtggcgcggc tccttgccgc gcgtcttgtc gcgcagcgcg
     2521 cgcaacaccg catgcggcgc gcacggtact tcgtcgatgc gcacgccgac cgcgtcgtac
     2581 accgcattgg ccaccgccgg cggaatcggg agccagggtc cctgtccgac ttctttcgcg
     2641 ccgaacggtc cgccgggctc cgccgcttcg atcagatacg actccacgtc gcacatctcg
     2701 aacgtggtcg gcgacttgta ctcgagcatg ctcgggtggt ggtgcacgag gcgccgctcg
     2761 gcgtcgcggt aggtcatctc ctccat
//
LOCUS       DASUCJ010000546         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1411811, whole genome shotgun sequence.
ACCESSION   DASUCJ010000546 DASUCJ010000000
VERSION     DASUCJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411811"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFU90_06480"
     CDS             complement(<1..789)
                     /locus_tag="VFU90_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5479460.1"
                     /translation="MTNASNSPGNVDVLIAGAGPVGLFLATECARRGLRFRIVEAQPT
                     QSQHSKALAIFPRTLEIFDMAGVSAPFLEAANRVTTVKLIAHAHTLGQIAFHPDDTPY
                     PFVAMVPQDVTEKLLVEALGGRGGAVEYETQLVSAVQDDAGVTATLDCRGTRSDVRAS
                     FLVGCDGAHSAVRHLLNLTFEGGEYPDSFMLADVHTNDALPADELQLCPNEAGPLAIF
                     PMNATRRRIVATVDAPEGDAPSLEFVRQTLAQRAPAGIEAHSLVW"
     gene            899..1267
                     /locus_tag="VFU90_06485"
     CDS             899..1267
                     /locus_tag="VFU90_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEU5479461.1"
                     /translation="MKNAFSLFASSVNRFVSSPAATGVAFAVVAVWAVFGPLTHYSTG
                     WQLVINTGTTIVTFLMVFVLNNAQYRDTAAINVKLDTLILAIEAADNRLVGLERMPVS
                     AAEQISEEIGQIGDEAKKKG"
BASE COUNT          246 a          375 c          462 g          219 t
ORIGIN      
        1 ccacacgagc gagtgcgctt caattccggc gggcgcgcgc tgcgcgagcg tttgccggac
       61 aaactccagc gagggcgcgt cgccctcggg tgcgtccacg gtggcgacga tccggcggcg
      121 ggtggcgttc atcggaaaga tcgccagcgg accggcttcg ttggggcaca gctgcagttc
      181 gtcggccggc aaagcgtcgt tggtgtggac gtcggccagc atgaacgaat cgggatattc
      241 gccgccttcg aacgtgaggt tcagcagatg acggaccgcg ctgtgtgcgc cgtcgcagcc
      301 caccaggaag gaagcgcgca catcggaacg cgttccgcga caatccagcg tggcggtaac
      361 gccggcgtcg tcctgcacgg ccgagacgag ctgcgtttcg tattcgaccg caccgccgcg
      421 gcccccgagc gcttcgacga gcagcttctc ggtgacgtcc tgcggcacca tcgcaacgaa
      481 cgggtaaggc gtgtcgtcgg gatgaaaggc gatttgcccg agggtgtgcg cgtgggcgat
      541 cagcttgacg gtggtgacgc ggttggcggc ctcgagaaac ggcgcgctca cgccggccat
      601 gtcgaaaatt tcgagcgtgc gcggaaaaat ggcgagcgct ttggagtgct gcgattgggt
      661 gggctgcgct tcgacgatgc gaaaacgcaa accgcggcgg gcgcattcgg tcgccagaaa
      721 caggccgacg gggcccgcgc cggcgatcaa gacgtcgacg ttgcctgggg agttcgatgc
      781 gttggtcatc gccgtcgatg atgtcacgaa tgcgcggcgt ttgtccacac cgggaaacgg
      841 tgaaaccggg aaagggcaag aaggtggatg gcagggtgtg gcggcgaagc gggccttaat
      901 gaaaaacgca tttagtctgt tcgcgtcgtc cgtcaaccgg ttcgtctcgt cgccggctgc
      961 gaccggggtg gcctttgcag tggtcgcggt gtgggcggtg ttcggcccgc tgacgcacta
     1021 ctccaccgga tggcaactgg tcatcaacac cggcacgacg atcgtcacgt ttttgatggt
     1081 gttcgtgctc aacaacgcgc agtaccgcga cacggccgcg ataaacgtca aactcgatac
     1141 cctgatcctc gcgatcgagg cagccgacaa ccggctggtt ggactcgagc ggatgccggt
     1201 gagcgcggcc gagcaaatca gcgaagaaat cggacagatc ggcgacgagg cgaaaaagaa
     1261 aggctagccc ggcgctttgg tggcgtcgag cgtgaagcac at
//
LOCUS       DASUCJ010000547         1245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1442551, whole genome shotgun sequence.
ACCESSION   DASUCJ010000547 DASUCJ010000000
VERSION     DASUCJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442551"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1245)
                     /locus_tag="VFU90_06490"
     CDS             complement(<1..>1245)
                     /locus_tag="VFU90_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479462.1"
                     /translation="FDRNLYGSLGLTNATFFDRASFGVDRLVVGTPTVTGADSWACRL
                     RPPRPIEAVAAEFPMSAAARTRLINLYTHPRDCLAGKTKAGKIAHLRRVSYRDYLIHD
                     AGLGGEAVKFFDGSTNDFWGMGPDIVPAYDALTSRYPGFAGLGLDDRIDPAYGEPYIY
                     HFPDGNASIVRLLVRDLIPPAASGKTMEDVVLASFDYAKLDDSGNPVRLRLNATAVKI
                     VDLADGVDVGYVRAGQLRRVRARHCVLACWNRMIPHIMPQLPLAQRQALVLAVKQPLV
                     YINVALRNWHPFVRLGVERINSPRAYFSDVKLDYPVAMGGYRNPRSPDEPILVHMLRV
                     PLAPNQGLSDVEQYKVGRYELLTTTFAEYETEIVGQLTRMLGAGGFDAARDIAGITVN
                     RWPHGYAYTFDTLFDKRTDPMP"
BASE COUNT          217 a          385 c          436 g          207 t
ORIGIN      
        1 agggcatcgg atcggtgcgc ttgtcgaaca gcgtgtcgaa agtgtacgca tacccgtgag
       61 gccagcggtt gacggtaatg cccgcgatat cgcgcgcagc atcgaagccg ccggcaccca
      121 gcatgcgcgt gagctggccg acgatctcgg tctcgtattc cgcgaacgtc gtcgtgagta
      181 gctcgtagcg cccgaccttg tattgctcga cgtcggagag cccctggttc ggcgccagcg
      241 ggacgcggag catgtggacc aggatgggct cgtccggcga gcgcggattg cgatagcctc
      301 ccatggcgac gggatagtcc agcttgacgt cgctgaagta ggcgcgcggg ctgttgattc
      361 tctcgacgcc gagcctgacg aaggggtgcc agttccgcag cgcgacgttg atgtagacca
      421 gcggctgctt caccgccagc acgagtgcct gccgctgcgc gagcggcaac tgcggcatga
      481 tgtgcgggat catgcgattc cagcacgcaa gcacgcaatg tcgcgcgcgc acccgccgca
      541 actggccggc gcgcacgtaa cctacatcga cgccgtcggc gagatcgacg atcttgaccg
      601 ccgtggcgtt caagcgcagc cgcacgggat tgccggagtc gtcgagcttc gcgtagtcga
      661 agctcgcgag cacgacgtcc tccatggtct ttccggaggc ggcagggggg atgaggtcac
      721 gcaccagcag gcgcacgatg gacgcgttcc cgtccgggaa gtgatagatg tagggttcgc
      781 cgtacgcggg atcgatccga tcgtcgagcc cgagtcccgc aaagcccgga tagcggctgg
      841 tcagggcgtc gtacgccggc acgatgtccg ggcccatgcc ccagaagtcg ttggtggagc
      901 cgtcgaagaa tttcaccgct tcgccgccca gcccggcgtc gtggatgaga tagtcgcggt
      961 agctcacccg gcgcagatgg gcgatcttcc cggccttcgt tttgccagcc aggcagtcgc
     1021 gaggatgggt gtaaaggttg atgagccggg tccgcgccgc cgccgacatc gggaattcgg
     1081 cagcgaccgc ctcgatggga cgcggcgggc gcagcctgca ggcccatgaa tcggcgccgg
     1141 tcaccgtcgg tgtgccgacg accagtcggt cgacgccgaa ggaggcccgg tcgaagaacg
     1201 tcgcgttggt cagcccgagc gatccataga gattgcgatc gaagt
//
LOCUS       DASUCJ010000548         1486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_21072, whole genome shotgun sequence.
ACCESSION   DASUCJ010000548 DASUCJ010000000
VERSION     DASUCJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21072"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..582)
                     /locus_tag="VFU90_06495"
     CDS             complement(97..582)
                     /locus_tag="VFU90_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5479463.1"
                     /translation="MTDTQRILDSIRRLVRLLRLTDRAAQSEVGLSAAQLFVLHELGK
                     TPSLSLSEVAERTRTDQSSVSVVVTRLVEGGYITRDRDKRDARRLVLNLTKSGRAVAE
                     KSPPAAQERILEALDKISPAERRRFADTFARVIDELGEGRAPAPMIFEDEPGRRTNPK
                     R"
     gene            624..1214
                     /locus_tag="VFU90_06500"
     CDS             624..1214
                     /locus_tag="VFU90_06500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5479464.1"
                     /translation="MQKRYDQAYFDRWYRGRTKIGPEPEVRRKVAMAVGVTEYLLRRG
                     IRNAIDIGCGEAPWFTHLVAVRPNVRYAGYDPSDYVVRTFGAARNIRRGSFGELAELN
                     IRERFDLVICADVLHYLGDDEIRDGLPQLVRLTRGAAFLEVLTREDEVVGDTTGMYRR
                     SADWYRDVFRRAKLRQVAPFLWAPPKIVEDAAALEL"
     gene            complement(1293..>1486)
                     /locus_tag="VFU90_06505"
     CDS             complement(1293..>1486)
                     /locus_tag="VFU90_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5479465.1"
                     /translation="IADSGPGIPEAVRGDLFTPFFSTKRDGRGLGLALVQEILTNHGA
                     AFWLQNRAGGGAEFGIRLP"
BASE COUNT          243 a          465 c          511 g          267 t
ORIGIN      
        1 gcggaacgcg tgcggcttcg agagtgggtg cgacagaggg agcgacaggg agacctacgg
       61 cggtagccgc ctggtcgaga tcggattcag ttttcttcac cgcttggggt tcgtcctcct
      121 gccaggttcg tcttcgaaaa tcatcggcgc gggggcgcgc ccttcgccga gctcgtcgat
      181 gacgcgggcg aacgtatccg cgaagcggcg ccgctccgcg ggcgagattt tgtcgagcgc
      241 ttcgaggatc cgctcctgcg ccgccggcgg cgacttctcg gcgaccgcac ggccgctctt
      301 cgtcagattg agcacgaggc gccgcgcgtc gcgcttgtcg cggtcgcggg tgatgtagcc
      361 gccctcgacg agacgcgtga cgaccaccga caccgagctc tgatcggtgc gcgtgcgctc
      421 ggcgacctcc gacagcgaga gcgacggcgt ctttccgagc tcgtgcagaa cgaagagctg
      481 cgctgccgac agtcccactt cgctctgcgc ggcgcgatcg gtcagccgca gaagacgaac
      541 gaggcgccgg atcgagtcga ggattcgctg cgtgtcagtc aatggcgatg tatagattac
      601 caaacgttgt acgcttgcgc ctcgtgcaaa aacgatacga ccaggcttat ttcgaccgct
      661 ggtatcgcgg acggacgaag atcggaccgg agccggaggt gcggcggaag gtggccatgg
      721 ccgtcggggt caccgaatac ctgctgcggc gcgggatccg gaatgcgatc gacatcggct
      781 gcggcgaggc gccgtggttt acgcatttgg tggccgttcg accgaacgtc cgttatgccg
      841 gctacgatcc gagcgattac gtcgtgcgca ccttcggcgc cgcgcgcaac atccggcgcg
      901 gttccttcgg cgagcttgcc gagctgaaca tccgcgagcg gttcgacctc gtcatctgcg
      961 ccgatgtcct gcactacctc ggcgacgatg agattcgcga cgggcttccg cagcttgtgc
     1021 ggctcacgcg cggcgcggcg tttctcgagg tcctgacgcg cgaggacgag gtggtcggcg
     1081 atacgaccgg aatgtatcgc cgttccgccg actggtaccg cgatgttttc cggcgggcga
     1141 agctgcgtca ggttgcgccg ttcctgtggg ccccgccgaa gatcgtggag gatgcggccg
     1201 cgctcgagct ttgatgtttc accgcagagg tgacgcaggg aacggagaaa ttaaccacag
     1261 gtgaaaactt ctccgtctcc tctgtgatga agtcaaggca gccggatgcc gaactccgcc
     1321 ccgccgcccg cccggttctg cagccagaac gccgcgccgt ggttcgtcag gatctcctgc
     1381 acgagcgcga gccccagtcc gcggccgtcg cgcttcgtgc tgaaaaatgg cgtgaagaga
     1441 tcgccgcgta cggcctcagg gataccggga ccgctgtcgg cgatgg
//
LOCUS       DASUCJ010000549         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1045034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000549 DASUCJ010000000
VERSION     DASUCJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFU90_06510"
     CDS             <1..1047
                     /locus_tag="VFU90_06510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5479466.1"
                     /translation="EREDDEFGSDAELFDGLDALPDDVSVDAFFARYERDAAMRPRIE
                     AARGFIEGFDAADPRIASARAIAQEWRSGIDSTAARPRAGYGPAFAFLLAECAKRGAD
                     VRFSATVTRIGWGDGGVTIALSDAGGRAYTLHARICVVTLPAGVLRQAGAAAAPAFDP
                     PLPAEKRGALAAIEMGNAVKAVLWFRTRFWEGLAGGRYRNGAFFQGGGAFAVFWTALP
                     RRDGPVTAWVGGPGSAALEGSPNAVVDRAVDEFGAIFGETELVRKEFATGAAHDWRND
                     PFARGAYSYVRVGGGDARTVLGASLGGVLFFAGEATALGGTSGTVEGALESGERAGRE
                     ALQALASYEGANRE"
     gene            1040..1579
                     /locus_tag="VFU90_06515"
     CDS             1040..1579
                     /locus_tag="VFU90_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479467.1"
                     /translation="MSESATALLGAWSTFFVMLGSSSAALTGLMFVVIGIVTSGVRAQ
                     SSHDGISVFSTPTVVHLCAALLISAIFLAPWRALTMPAVLTALAGGAGIAYMLLLAYR
                     TRKFDRYRADAEDWAWYNVLPFVAYGALCAGGLLALGNPARATFTIAGAAVLLVFIAI
                     RNAWDIVTYIATGQMNSDR"
     gene            complement(1659..1895)
                     /locus_tag="VFU90_06520"
     CDS             complement(1659..1895)
                     /locus_tag="VFU90_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479468.1"
                     /translation="MVSRGCGGLVAHVAAEVRVAVHGGATGGAGTTGGAAPKSTTPAM
                     AVEPARADNHLLFIDKHPPGVIVVIAIRRYTASY"
     gene            complement(2089..2382)
                     /locus_tag="VFU90_06525"
     CDS             complement(2089..2382)
                     /locus_tag="VFU90_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU5479469.1"
                     /translation="MPGEQALRIDEATTHEILARLRRVEGQVRAVQRMIQERRDCHAI
                     ANQMAAARGALERATVQLMATSMAQCLRPNGKQSVVDERELHRLTETFVKLLS"
BASE COUNT          376 a          783 c          861 g          438 t
ORIGIN      
        1 gaacgcgaag acgacgagtt cggatccgac gccgaactgt tcgacggtct cgatgcgctg
       61 ccggacgacg tcagcgtcga cgcgtttttc gcgcgctacg aacgcgacgc ggcgatgcgg
      121 ccgcgtatcg aagcggcacg cggattcatc gaaggattcg acgccgccga tccgcggatc
      181 gcgagcgcgc gcgcgatcgc gcaagaatgg cgctccggga tcgattcgac cgccgcccgt
      241 ccacgcgccg gttacgggcc ggcgtttgcg tttctcttgg cggagtgtgc gaagcgcggc
      301 gcggacgtcc gcttttccgc aaccgtgacg cggatcggct ggggagacgg cggcgtgacg
      361 attgcgctaa gcgacgccgg cggccgcgcg tacacgctgc acgcgcgaat ttgcgtcgta
      421 acgctgccgg ccggcgtgtt acggcaggcc ggcgcggcgg ccgcgccggc attcgatccg
      481 ccgctgccgg cggaaaaacg gggcgcgctc gccgcgatcg agatgggaaa cgccgtcaaa
      541 gcggtgctgt ggttccgaac gcgcttctgg gaaggtctcg ccgggggacg ctatcgcaac
      601 ggcgcgttct ttcaaggcgg cggcgccttc gcggtatttt ggaccgcgct tccgcggcgc
      661 gacggtccgg ttaccgcgtg ggtcggcgga ccgggaagcg ccgcgctgga gggctcgccc
      721 aacgccgtcg tcgatcgagc ggtcgacgaa ttcggcgcga ttttcggcga aaccgagctc
      781 gtgcgtaagg aattcgcgac cggcgcggcc cacgactggc gcaacgaccc gttcgcccgc
      841 ggcgcttaca gctacgtacg ggtaggcggc ggcgacgcgc gcacggtgct gggcgcttcg
      901 ctgggcggcg tgctgttttt tgccggtgaa gcgaccgcgc tgggaggcac gagcggaacg
      961 gtagaaggcg cgttagaaag cggcgagcgc gccggccggg aggcgttgca ggcgctggcg
     1021 tcttacgagg gggccaatcg tgagtgaatc cgcaacggcg ctgctgggcg cgtggtcgac
     1081 gttcttcgtg atgctggggt cgtcgtcggc cgcactgacc ggcctcatgt tcgtcgtgat
     1141 cgggatcgtc acgagcggcg tgcgcgcgca aagctcgcac gacggcattt cggtgttcag
     1201 cactccgacc gtcgtgcact tatgcgccgc cctgctgatt tcggcgatct tcctggcacc
     1261 ctggcgcgcg ttgacgatgc cggccgtgct gacggcgctg gccggcggcg ccggcatcgc
     1321 gtacatgctg ctgctggcgt accggacgcg caagttcgac cgctatcgtg ccgacgccga
     1381 agattgggcg tggtacaacg tgctgccgtt cgtcgcgtac ggtgcgctgt gcgcgggcgg
     1441 cctgctggcg ctaggtaatc cggcacgcgc gacgttcacg atcgccggcg ctgcggtgct
     1501 gctggtattc atcgcgatcc ggaacgcgtg ggatatcgtt acctatattg cgaccggtca
     1561 gatgaactcg gatcgctgat tctctcaaga gacaggccgg tgctcgctcg ttgcaaaacg
     1621 atgacgctcg ttagttttcg acaccctcta atgcgacgct aatatgaggc ggtgtagcga
     1681 cgaatcgcga tgaccacaat tacgcccgga ggatgtttat cgatgaatag caagtggtta
     1741 tcagctctcg ccggttcgac cgccatcgcg ggcgtcgtgc tcttgggcgc ggcaccgccg
     1801 gtcgttccgg cgccgccggt agcgccgccg tgtacggcta cgcgaacctc ggcggcgacg
     1861 tgcgcgacga ggccgccgca tccgcgcgag accatgatac cgttccaacg cgtcgatccg
     1921 atgcaggtgg ggaacccacg gacgatcgcg ccgtcgcgca cggtggtggc accgccgcgc
     1981 gctcccagct ccgccggtaa ggcggtatcg cgaccgccgc aatagccgaa ccgcccaacg
     2041 cgccctccgc aaaaaaaacg gagggcgcgc cgctttaaag cattcccttt acgacagcag
     2101 ctttacgaag gtttccgtga gccggtgcag ctcccgctcg tcgacgacgc tttgcttacc
     2161 gttggggcga agacactgcg ccatactcgt cgccatcagc tgaacggtcg cgcgctcgag
     2221 tgcgccccgc gccgcggcca tttgattggc gatcgcgtga cagtcgcggc gctcctggat
     2281 catccgctgc accgcccgga cctggccttc gactcggcgg aggcgagcga gaatctcgtg
     2341 cgtcgtcgct tcgtcgatgc gcagggcctg ttcgccgggc attttccttg ccatacgtgc
     2401 cgactatagc gcaggtccgc ggtgcttgtc tataccctgg ggggtatggt ataaggag
//
LOCUS       DASUCJ010000550         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1182716, whole genome shotgun sequence.
ACCESSION   DASUCJ010000550 DASUCJ010000000
VERSION     DASUCJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182716"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1117
                     /locus_tag="VFU90_06530"
     CDS             104..1117
                     /locus_tag="VFU90_06530"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5479470.1"
                     /translation="MNHPPLIFSGNSNPQLAEEIAKRLKLHVGKALVGEFKNAETRIE
                     IGENVRGSEVFVVQSICKMPNGKSVNDALMELLLMIDALRRASAARITSVIPYYGYAK
                     QDKKTKGREPISAKVVANLLKVCGSRRIVTMDLHAAQIQGFFDIPVDNLMATHVLCNY
                     LKKAGLCDDTIVVVSPDAGGVHRAEIFAKRLGSSLAIVFKRRPEPDVSEVTDIVGDVS
                     GKVAVIVDDMISTGGTLAKAAEAIKSRGATRVYTVASHGIFADDAVDVLERSGIEKVI
                     VTNTIPFTNVERHPKFVQLSIAQTFADAISRITTNRSVSELFTGDEPAKLDPPALLAE
                     TAV"
     gene            1140..1793
                     /locus_tag="VFU90_06535"
     CDS             1140..1793
                     /locus_tag="VFU90_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5479471.1"
                     /translation="MANKELKLAVETRTSVGTTRSNALRRVGKFPAVLYGHGAAPEHV
                     AIDAHAFEELLHHGGRNAIITLTGGQKKGETALVRVVQYHPLSHRVVHVDLQRVSANE
                     TIASKLAVVTAGVARGIREAGGVMDVVTHELEIEGPANRIPENIEIDVTELGLHEHVT
                     AADVKLPDGFKMLTPPETTVVSIEPSRTERELEEAAAGPVEAAEPEVIGAAPETEAE"
     gene            1821..2375
                     /gene="pth"
                     /locus_tag="VFU90_06540"
     CDS             1821..2375
                     /gene="pth"
                     /locus_tag="VFU90_06540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5479472.1"
                     /translation="MVVGLGNPGKEYASTRHNVGFMVADEVARRYGVSSWKKKDSAQQ
                     AYDSARRVVFVKPLSYMNLSGTPVRLISSWYRTPPEDVLAIVDEMDLPFGTLRMRPFG
                     GHGGHNGLRSMIATIGDGFPRLRVGVGRPEYASVEHVLGPFTIAERKVLPQIVDAAAD
                     AVELWLDEGLDPAMRFANTWAAPA"
BASE COUNT          450 a          797 c          771 g          365 t
ORIGIN      
        1 aaaaggtcgc ccctggcggg ctcgtcccac ggggagcgag gaggcgcgga gcggccgcgt
       61 gaacacctgt ggtccgctgc ttttcgcccc ggggagaaca gccttgaacc atccgccgct
      121 catcttcagc ggaaactcca atccgcaact cgccgaagag atcgccaagc gcctcaagct
      181 ccacgtcggc aaggcgctgg tcggcgaatt caaaaacgcc gagacccgaa tcgagatcgg
      241 cgagaacgtg cgcgggtcgg aagtcttcgt cgttcagtcg atttgcaaga tgcccaacgg
      301 caagagcgtc aacgacgccc tgatggagct gctgctgatg atcgacgcct tgcggcgggc
      361 ctcggccgcc cggatcacgt cggtcattcc ctactacggc tatgccaagc aggacaagaa
      421 gacgaaaggg cgcgagccga tctcggccaa ggtggtcgcc aacctgctca aggtctgcgg
      481 atcgcggcgc atcgtcacga tggacctgca cgccgcgcag attcagggct ttttcgacat
      541 cccggtcgac aacctgatgg ccacccacgt gctctgcaac tacctcaaga aggccggcct
      601 ctgcgacgac acgatcgtcg tcgtttcgcc ggatgccggc ggggtgcatc gcgccgagat
      661 cttcgccaag cgcctgggca gctcgctggc catcgtgttc aagcggcgcc ccgagcccga
      721 cgtttccgag gtcaccgaca tcgtcggcga cgtttccggt aaggtcgcgg tgatcgtgga
      781 cgatatgatc tcgaccggcg ggacgctcgc gaaggcggcc gaggcgatca agtcgcgcgg
      841 ggcgacccgg gtctacacgg tggcctctca cggcatcttc gccgacgacg cggtcgacgt
      901 gctcgaacgc tcgggcatcg agaaggtcat cgtgaccaat acgattccgt ttaccaacgt
      961 cgaacggcac ccgaagttcg tgcagctgtc gatcgcacaa acgttcgccg acgcgatcag
     1021 ccggatcacg accaaccgtt cggtctccga actcttcacc ggcgacgaac cggcgaaact
     1081 cgacccgccg gcgctgctgg cggaaaccgc agtctaagaa aggcatcctc gaaatcccta
     1141 tggctaacaa agagctcaag ctcgccgtcg aaacgcgcac cagcgtcggc accacccgct
     1201 cgaacgcgct ccgtcgcgtc ggcaagtttc ccgccgtcct ctacggccac ggcgccgccc
     1261 ccgagcacgt cgcgatcgac gcgcacgcgt tcgaagagct gctccatcac ggcggccgaa
     1321 acgcgatcat cacgctcacc ggcgggcaga aaaaaggcga aaccgcactc gtacgcgtcg
     1381 tgcagtacca tccgctttcg caccgggtcg tgcacgtcga tctgcagcgg gtttcggcaa
     1441 acgagacgat cgcttcgaaa ctcgcggtcg tgaccgccgg cgtcgcccgc ggcatccgcg
     1501 aggcgggcgg cgtgatggac gtcgtgacgc acgagctgga aatcgagggg cccgcaaacc
     1561 gcatccccga gaatatcgaa atcgacgtga ccgagctggg gctgcacgag cacgtcaccg
     1621 ccgccgacgt gaagctgccg gacggcttca agatgctcac gccgcccgaa acgacggtcg
     1681 tttcgatcga gccgtcgcgt acggaacgcg agctggaaga ggccgccgcc ggcccggtcg
     1741 aagcggccga acccgaggtc atcggcgccg ctcccgagac cgaagcggag taacgcctgg
     1801 ccgagcaacg cgcgccgcgg ctggtcgtcg gactcggcaa tccgggaaaa gagtacgcct
     1861 cgacgcgcca caacgtcggc ttcatggtcg ccgacgaggt ggcgcggcgc tacggcgtct
     1921 cgagctggaa gaaaaaggac agcgcgcagc aagcctacga ttccgcccgg cgggtcgtgt
     1981 tcgtgaagcc gctgtcgtac atgaacctga gcggcacccc ggtccggctg atttcttcgt
     2041 ggtatcgcac gccgcccgag gacgtgctgg ccatcgtcga cgagatggac ctcccgttcg
     2101 gcacgctgcg gatgcggccg ttcggcggtc acggcggtca caacgggctg cgttcgatga
     2161 tcgcgaccat cggcgatgga tttccgcggc tgcgcgtcgg cgtcggccgc ccggagtacg
     2221 cgtcggtcga gcacgtgctc gggccgttca cgatcgccga acgaaaggtc ttgccgcaga
     2281 tcgtcgacgc cgccgccgac gcggtggagc tttggctcga cgaagggctg gatccggcga
     2341 tgcgctttgc gaatacgtgg gcggcgccgg cgtaaaaccg gcc
//
LOCUS       DASUCJ010000551         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1060407, whole genome shotgun sequence.
ACCESSION   DASUCJ010000551 DASUCJ010000000
VERSION     DASUCJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060407"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..473)
                     /gene="pal"
                     /locus_tag="VFU90_06545"
     CDS             complement(21..473)
                     /gene="pal"
                     /locus_tag="VFU90_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU5479473.1"
                     /translation="MFAAALAISACANNPDQNAQANAAGMATPGSQQDFVVNVGDRVF
                     FETDSTDLTPTSRGTLDKQAQWLNQYSQYGQFTIEGHADERGTREYNIALGARRAQAV
                     RDYLASRGVSPSRMRTISYGKERPVAVCNAISCWSQNRRAVTVLNTSS"
BASE COUNT          185 a          315 c          332 g          186 t
ORIGIN      
        1 cttccccccg tgtcccctgc tcaggaactg gtattgagca ccgtcaccgc acgccggttc
       61 tgcgaccagc atgaaatggc gttgcacacc gcgaccgggc gctccttgcc gtaggagatc
      121 gtgcgcatgc ggctcggcga gacgccgcgc gaggcgaggt agtcgcggac cgcctgggcg
      181 cgccgagcgc cgagcgcgat gttgtattcg cgcgtgccgc gctcgtcggc gtggccttcg
      241 atcgtgaact ggccgtattg cgaatactga ttgagccact gcgcctgctt gtcgagcgtg
      301 ccgcgcgatg tgggcgtcag gtccgtcgag tcggtctcga agaagacgcg gtcgccgaca
      361 ttgacgacga aatcctgctg gctgcccggc gtcgccatgc cggcggcgtt cgcctgggcg
      421 ttctggtcgg gattgttggc gcaggccgag atcgccagcg cggcggcgaa caccgcggcg
      481 aatttcgccc cgtgcagcat tttcctgaga tcgatcatac cggttgactc cattgacgcg
      541 cagcgaagca cgttcacccg tacgcacatt tcacaaagcg cattccgcgt ccggacgcgg
      601 tgcgcgactt cggaaatttc tagcagcgcc ttcgttaagc gaacgttcca tcaaccttga
      661 tggaaccttg ccgggatcga cttaagacga caggcccccg taaaccgtct gtgatggtta
      721 agagacggtg aatttaaggc agcggcatga cggcgcagca agccgcgacg cgctgtggac
      781 gcagcgcaac acttttgtgc gcgaacaaac atcgagaagc accaactacc gttcgtcccg
      841 gcgatcggga ccggtgccag caaaccgtgc ggtcgctacg acagcagcgg cgaccaagcc
      901 gggtcggagc cgaagctcgg cgtcggctcc ttgagttcgt tccgtccggt gatgtcgatg
      961 gtgtagagat acggaccggg cccgaggtcg cggaaaaaca tgatgacgcg gccgttcg
//
LOCUS       DASUCJ010000552         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_510247, whole genome shotgun sequence.
ACCESSION   DASUCJ010000552 DASUCJ010000000
VERSION     DASUCJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510247"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFU90_06550"
     CDS             <1..847
                     /locus_tag="VFU90_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5479474.1"
                     /translation="DQHPMLRLNLDAQLLGEQPVYNTVAELKGTEHPDEYVMLSAHFD
                     SWDGSSGATDNGTGTLTMLEAMRILQKVFPHPKRTILAGHWSGEEEGEVGSKAFTEDH
                     PEVLKGLQALFNQDNGTGRIVRLSGAGLPDASAHIARWLDQIPLELRNQIQFGGAGFP
                     AGGGSDDYSFSCYGLPAFGLGALPWDYGNYTWHTNRDTPDKIVYDDLKSNATLTAMLA
                     YLASEDPTMIKRDRVDLVAAAKRLEAELPPPTAGRNAAFRFRRLPTTWPECTKAPRST
                     KPRLK"
     gene            complement(855..>1768)
                     /locus_tag="VFU90_06555"
     CDS             complement(855..>1768)
                     /locus_tag="VFU90_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5479475.1"
                     /translation="ADYHHMVGFAHHALDWLSHKWPGVPYPYEKSTVVQGSADMEYPM
                     MVNDGSTPDTIFSRFVVEHEIAHTYFPFYMGINETRYPFMDEGWATTFEYLIGTSDLG
                     PARATNFFKQFRVAGWITDPSPLEDLPIVTPADVLKDAAYGNNAYGKAALGYLALKQL
                     LGDDTFKRCLHAFMDRWHGKHPTPWDFFYTFNDVSGRNLDWFWNSWFFSHAYIDLGIT
                     GARRGANGWTVTLENVGGMPAPVDLVVRYTDATVDSVHETPAIWRTNLHRATVTVPAK
                     KAVTSIALDHGIWMDADSTNDRWTVKR"
BASE COUNT          322 a          588 c          556 g          302 t
ORIGIN      
        1 cgaccagcat ccgatgctgc ggctcaacct cgacgcgcag ttgttaggcg agcagccggt
       61 ctacaacacc gtcgccgagc tcaagggcac cgagcatccc gacgagtacg tgatgctgtc
      121 ggcgcacttc gactcgtggg acggctcgtc gggcgccacc gacaacggca ccggcacgct
      181 cacgatgctc gaggcgatgc gcatcctgca gaaggtgttt ccgcatccca agcggacgat
      241 tctcgccggg cactggagcg gcgaggaaga gggcgaggtg ggctcgaagg cgttcaccga
      301 ggatcacccc gaggtgttga agggcttgca ggcgttgttc aaccaggaca acggcaccgg
      361 acgcatcgtg cgcttgagcg gcgcgggtct gccggatgcg tcggcgcaca tcgcgcgctg
      421 gctcgatcag attccgctcg agctccgcaa ccagatccaa ttcggcgggg ccggtttccc
      481 ggccggcggc ggcagcgacg actactcgtt ctcctgctac ggactaccgg cgttcgggtt
      541 aggcgcactg ccgtgggact acggcaacta cacgtggcac acgaaccgcg acacgccgga
      601 caagatcgtc tacgatgacc tcaagtccaa cgcgacgctg accgcgatgc tggcgtatct
      661 ggcgtccgag gatccgacga tgatcaagcg cgatcgcgtc gacctggtgg ccgccgcgaa
      721 gcggctcgaa gccgagctgc cgccacccac cgccggccgc aacgccgcct tccggttccg
      781 ccgcctgcca accacgtggc cggaatgcac gaaggcgccg cgcagcacga aaccgcggtt
      841 gaaatgagcg ccgcctaacg cttgaccgtc cagcgatcgt tggtgctgtc cgcgtccatc
      901 caaatgccgt ggtcgagcgc gatcgacgtc accgctttct tcgccggaac ggtgaccgtc
      961 gcgcggtgca gattcgttcg ccagatggcg ggcgtctcgt gcacactgtc gactgtcgca
     1021 tcggtgtagc gcaccaccag gtccacgggc gccggcatgc cgccgacgtt ctcgagcgtc
     1081 accgtccagc cgttcgcacc gcgccgggcg ccggtgatgc ccagatcgat gtacgcatgg
     1141 ctgaagaacc agctgttcca gaaccaatcc agattgcggc ccgacacatc gttgaacgtg
     1201 tagaagaaat cccacggcgt cggatgcttg ccgtgccagc ggtccatgaa cgcatgcagg
     1261 catcgcttga acgtgtcgtc gcctaacaat tgcttcagtg ccaggtagcc gagcgccgct
     1321 ttgccgtacg cgttgttgcc gtacgccgca tccttgagca cgtcggccgg cgtcacgatc
     1381 gggaggtcct cgagcggcga cggatccgtg atccatccgg ccacacggaa ttgcttgaag
     1441 aagttggtcg cgcgcgccgg gcccaagtcc gatgtgccga tgagatactc gaacgtcgtc
     1501 gcccaaccct cgtccatgaa cgggtagcgc gtctcgttga tccccatgta gaacgggaag
     1561 tacgtgtgcg cgatctcgtg ctccaccacg aaccgcgaga aaatcgtgtc cggcgtcgat
     1621 ccgtcgttca ccatcatcgg atactccatg tccgccgagc cctgcaccac cgtgctcttc
     1681 tcgtacggat acggaacgcc cggccatttg tgcgacaacc agtcgagcgc gtggtgcgcg
     1741 aagccgacca tgtggtgata gtcggccg
//
LOCUS       DASUCJ010000553         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_128384, whole genome shotgun sequence.
ACCESSION   DASUCJ010000553 DASUCJ010000000
VERSION     DASUCJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128384"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1080
                     /locus_tag="VFU90_06560"
     CDS             <1..>1080
                     /locus_tag="VFU90_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479476.1"
                     /translation="GVALVFIGFIPFLRALGASDRVSYTAPALALTVFFLLPFFIVET
                     FTGALAKDFTIFVLAGLLIVMGATWLTMYNTDIILRGAMAVFGRVRWLAPVLKTAIAY
                     PLSSRFRTGITFAMFTLIVFTLVTMATIITSVNRVIDNYEPFAGGYDIHAETAPISPI
                     NDLGGAIKNDPSLKASDFRSVASGSLLQMKARQAGTNGEFQDYLVRGMDNSFLENNTY
                     GFALTAPGYNSARDVWRALESDPNLAVVDATVVPHRTNYSAALGGYDFRLSGFYFEDG
                     KLPPTQIEIQDERTGTIRTLTVIGVLNEVASPFVTLGITSSQASMQAAYGDRAQPNMH
                     FIQLAPGVDAKTAAKSLESAFLPNGL"
BASE COUNT          182 a          360 c          353 g          185 t
ORIGIN      
        1 cggcgtcgcg ctcgtgttca tcggcttcat ccccttcctg cgcgcgctcg gagccagcga
       61 ccgcgtgtcg tacacggcgc cggcgctggc gctcacagtg ttcttcctgc tgccgttctt
      121 catcgtcgag acgttcacgg gtgcgctcgc gaaggacttc acgatcttcg tgctcgccgg
      181 gctgctgatc gtgatggggg cgacctggct gaccatgtac aacacggaca tcatcctgcg
      241 gggcgcgatg gccgtgttcg ggcgcgtgcg ctggctcgcg ccggtgctga agacggcgat
      301 cgcgtacccg ctgagcagcc gcttccgcac ggggatcacg ttcgcgatgt tcacgctcat
      361 cgtgttcacg ctcgtgacca tggcgaccat catcacgtcg gtgaaccgcg tcatcgacaa
      421 ctacgagccg ttcgcgggcg gctacgacat ccatgcggag accgcgccga tcagtccgat
      481 caacgatctg ggcggcgcca tcaagaacga cccgtcattg aaggcgtccg acttccggag
      541 cgtggcgtca ggctcgctgc tgcagatgaa ggcgcggcag gcgggcacga acggcgagtt
      601 ccaggactac ctcgtgcgcg ggatggacaa ctcgttcctc gagaacaaca cgtacggctt
      661 cgcgctgacg gcgcccggat acaacagcgc gcgcgacgtc tggcgcgcgc tcgagtcgga
      721 cccgaacctc gccgtcgtcg acgcgacggt cgtgccgcac cgcacgaact acagcgcggc
      781 gctcggcggc tacgacttcc gcctcagtgg gttctacttc gaggacggga aactgccgcc
      841 gacgcagatc gagatccagg acgagcggac gggcacgatt cgaacgctga cggtgatcgg
      901 cgtgctcaat gaagtagctt cgccgttcgt gacgctcggc attacgagct cgcaggcgtc
      961 catgcaggcg gcatacgggg accgcgcgca gccgaacatg cacttcatcc agctggcgcc
     1021 gggcgtcgac gcgaagaccg ccgcgaagtc actggaatcg gcgttcctgc ccaacggcct
//
LOCUS       DASUCJ010000554         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_846491, whole genome shotgun sequence.
ACCESSION   DASUCJ010000554 DASUCJ010000000
VERSION     DASUCJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846491"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..657)
                     /locus_tag="VFU90_06565"
     CDS             complement(100..657)
                     /locus_tag="VFU90_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479477.1"
                     /translation="MTTGASTSTIIIGIIALIVIAAIIVAAVRVTRSRRLARRFGPEY
                     DHAVREHGDRGAAERELANREARVRRLHIEELPAGAKARYVEEWRTVQSRFVDEPSLA
                     IAEADRLVSSVMRDRGYPLGNFEQRAGDLSPDHPRVIEDYRIAHDIVRRSERGEVPTE
                     DLRQAMVHYRTLFSDLVEADERTPR"
     gene            723..>1586
                     /locus_tag="VFU90_06570"
     CDS             723..>1586
                     /locus_tag="VFU90_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU5479478.1"
                     /translation="METEQTTHPPSRAERSRHILAILAKHGLSAAGAGLTSRNGRSEA
                     TAREVRLACEELGTTFIKLAQLLSARSDLLPTDYRDELAKLQDDVPPVEPALVERVFM
                     EEFGAHPLELFASFDLVPAASASIGQVHDARLADGREVAVKIRKPGVRELVERDLEIL
                     RGLTGAAERYFPSAAGYDLDGLLQEFDDTIHDELDYTREARNIDMFAGFFSDEPGIEV
                     PEVVRELSTSQILTMTRAGGSRPEDVGGMTPRRRVAAAGRIARFVLEPALVHGVFHAD
                     PHEGNVLVRRNG"
BASE COUNT          258 a          485 c          547 g          296 t
ORIGIN      
        1 gatcggtttc cgccgagccg cgcgtcgttt gctcgcggtc gcgcgtttgc gtcgcggaag
       61 catcggaacg gtcggctttc tcggggcggt cgttgtcgtt catcggggag tcctttcgtc
      121 ggcttcaacg aggtcgctga atagggtgcg gtagtgtacc atcgcctggc gcaggtcttc
      181 ggtcggcact tcgccgcgtt cgctgcgtcg cacgatgtcg tgagcgatgc gataatcttc
      241 gatcacgcgc ggatggtccg gtgagagatc gccggcgcgc tgttcgaaat tcccaagcgg
      301 gtagccgcga tcgcgcatga cgctcgaaac gagccgatcg gcttccgcaa tggcgagcga
      361 gggttcgtcg acgaaccggc tctgcacggt gcgccactct tcaacgtacc gcgctttggc
      421 gccggcggga agctcctcga tgtgaaggcg gcggacccgc gcttcgcggt tggcgagttc
      481 gcgctcggcg gcgccgcgat cgccgtgctc gcgcacggca tggtcgtact cggggccgaa
      541 gcgccgtgcg agccgtcgcg agcgggtgac gcgaacggcc gcgacgatga tcgcagcgat
      601 gacgatgagg gcgataatgc cgatgatgat tgtcgaggtt gacgctccgg tcgtcatggt
      661 ggtcctacct ccgctgaaag atttcactct tgttacccgc atttgggtac aagtaagcca
      721 cgatggaaac cgagcaaacg acccacccgc cgagccgcgc cgagcgatcg cgtcacattc
      781 tcgcgatact ggccaagcat gggcttagtg ccgcgggtgc tgggctaacg tcgcgcaacg
      841 ggcgttcgga ggcgacggcg cgcgaagtgc gcctcgcctg cgaagagcta ggcacgacct
      901 tcatcaaatt ggcacaattg ctttcggcac gcagcgattt gctgccgacc gactatcgcg
      961 acgagctggc aaagctgcaa gacgacgtac cgccggtcga gcccgcgctc gtcgagcggg
     1021 tcttcatgga agaattcgga gcgcatcccc tggaattgtt cgcgtccttc gacttggtgc
     1081 cggccgcgtc ggcgtcgatc gggcaagtgc acgacgcgcg gctcgcggat gggcgcgagg
     1141 tcgcggtgaa gattcgcaaa cctggggtgc gcgagctcgt cgagcgcgac ctcgagattc
     1201 tgcgcggact gacgggcgcc gccgaacggt atttcccttc ggcggcgggt tacgatctcg
     1261 acggcctact gcaagagttc gacgacacga ttcacgacga gctcgattac acgcgcgaag
     1321 cgcgcaatat cgatatgttc gcgggattct tctccgacga gccgggtatc gaggtgccgg
     1381 aagtcgtgcg cgagctatcg acatcgcaga tcctcacgat gacgcgcgcc gggggcagcc
     1441 gtccggagga tgtcggcggt atgacgccgc gccgccgcgt ggccgcggcc ggacggatcg
     1501 cgcgctttgt gctggagccg gccttggtcc atggcgtttt ccacgccgat cctcacgagg
     1561 gcaacgtcct cgtccggcgc aacggt
//
LOCUS       DASUCJ010000555         1499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1090945, whole genome shotgun sequence.
ACCESSION   DASUCJ010000555 DASUCJ010000000
VERSION     DASUCJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090945"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1298
                     /locus_tag="VFU90_06575"
     CDS             255..1298
                     /locus_tag="VFU90_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5479479.1"
                     /translation="MGKVTVSDIAAASGLSRAAISLVLQDSNRVSAATKVRVRQIMSE
                     LGYVYDRRAANLRTQKSMIVGLIVTNVRNPYFAELTMSIEQRLQTAGYTLLLGYSHDE
                     DDRQRRLLATMTEHRVDGIVLLPAAETTRASLAGLDGTPHVLIARRVRGHNADYAGVA
                     NVKAGALLGRHLIDIGARDIAFVGGPAGSIARTERERGLRRELETAGVSIAPQNSIGS
                     PSTPSGGIEAAEKLLEQGAPPDVIVCYSDAVAFGVLHTLHERGLRIGHDVAVAGFDDT
                     TEARHQNPPLTSVATFPERIGEEAARLLLERIDQPDRVPSAFIASPELNVRESTSTWT
                     PLAARRRPSRSGR"
     gene            1295..>1499
                     /locus_tag="VFU90_06580"
     CDS             1295..>1499
                     /locus_tag="VFU90_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5479480.1"
                     /translation="MSAALRWGLIGGSDIAATRMIPAMRASGHAVVGIMSGDSERAAR
                     YAERNQLEFSTSEIGALLDRSDID"
BASE COUNT          287 a          491 c          477 g          244 t
ORIGIN      
        1 accgcacccg cggtcagcag atgggcgagt cgccgaggga ttcccatgcc acaacccaag
       61 ccaacggcca gcgggcggca ccctggcccc gaggctaggt tgccctcgcg acagcgcgcc
      121 tcggataacg aattcggcac gacatgttga catcgaacgg gcggcgcaac agacttctgc
      181 taaatcgatc cagcagatgg ctgctagatc gtttgagcag acgaagtcga cgcgagcgct
      241 agggggcaag gaacgtgggc aaggtcacgg tcagtgacat cgcggccgcg agtggtttgt
      301 cacgtgctgc catcagcctc gtgttgcaag acagcaaccg ggtctcggca gccacgaagg
      361 tgcgtgttcg gcagatcatg tccgagctcg gctatgtgta cgaccgccgc gccgccaatc
      421 tgcgcaccca gaagtcgatg atcgtgggat tgattgtcac taacgtgcgc aacccgtact
      481 tcgccgagct cacgatgtcg atcgagcagc ggctgcaaac cgccggttac acactgctgc
      541 tgggctacag ccacgatgaa gatgatcgtc agcgtcgctt gctcgccacg atgaccgagc
      601 atcgcgtcga cggcatcgtg ctgcttcctg ccgcggagac cacccgagcg agtcttgccg
      661 gattggacgg cacgccacac gtcctcatcg cgcggcgcgt ccgcggtcac aacgctgact
      721 acgccggcgt cgccaacgtg aaggcgggtg ccttgttggg tcggcacctg attgacatcg
      781 gcgcccgcga tatcgccttc gtcggaggcc cagccggctc tatcgcgcgc accgagcgcg
      841 agcgcggctt gcgcagagaa cttgagacgg ctggtgtgtc aatcgccccg caaaactcga
      901 tcggttcgcc gtcgacgccg tcaggcggga tcgaggcggc cgagaagttg ctagaacaag
      961 gcgctccacc ggacgtcatc gtttgttaca gcgacgccgt cgccttcggc gtgctgcaca
     1021 ccttgcatga gcgcggtctg cgcattggac acgacgtcgc agtcgccgga ttcgacgaca
     1081 caacggaggc gcgtcaccaa aatcccccgc tgacttccgt cgcgacgttc ccggaacgga
     1141 tcggcgagga agcggcccgc ttgcttcttg agcggatcga tcaacccgat cgcgtgccga
     1201 gcgcgttcat cgcgtcgccg gaactcaacg ttcgcgagtc cacatcgacg tggacgccgc
     1261 tcgcggcacg ccgacgccca tcacggagcg gcagatgagc gcggccctga gatggggact
     1321 gatcggcggg agcgacatcg ccgccacccg aatgattcca gcgatgcgcg cgtcgggcca
     1381 cgccgtcgtt ggaatcatga gtggcgactc cgaacgcgca gccagatacg ccgagcggaa
     1441 tcagctcgag ttctccacat cggagatcgg cgccctactc gaccgcagcg acatcgacg
//
LOCUS       DASUCJ010000556         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1351067, whole genome shotgun sequence.
ACCESSION   DASUCJ010000556 DASUCJ010000000
VERSION     DASUCJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351067"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>1144)
                     /locus_tag="VFU90_06585"
     CDS             complement(138..>1144)
                     /locus_tag="VFU90_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5479481.1"
                     /translation="FGVLFKNAEAIETLRRVDTLVVDKTGTLTQGRPVLQDVVALDAR
                     ADAAIVQLAASLERGSEHPLATAIVQGAQERGIALKEVTGFEAAPGKGVRGQIDDHQV
                     ALGNAALMSTLGVDVTVSTSHVERLRAAGKTAMFVAVDGRLAGLVAVADPIKDTTPPA
                     IKALHDAGVRIVMLTGDNETTARAVASQLGIDEIVADVLPDQKAQVVKRLQSEGKFVA
                     MAGDGINDAPALAQAQVGIAMGTGTDIAMESAGVTLVKGDLRGIVRARRLSEATMRNM
                     YQNLVWAFIYNVFGVPIAAGVLYPAFGMLLSPMIAAAAMSFSSVSVIGNALRLRAVRV
                     "
BASE COUNT          187 a          389 c          368 g          200 t
ORIGIN      
        1 ctcgcggcgc tcgcgcaccg cgtaggttcg gcatacgcat ggctgacgtt ccgcacccct
       61 gcaaacgaca cgttcgattc gctgcaagca ttgcgagaac cgtcgtgatg ccatccacgt
      121 gacccgaagc aacagattca aacccgaacg gctctcaggc gaagcgcgtt cccgatgacc
      181 gacacagagc tgaacgacat cgcggcggcc gcgatcatcg gtgagagcag catgccgaaa
      241 gccggataca agacgccggc tgctatcgga acgccgaaga cgttgtagat gaacgcccag
      301 acgaggttct ggtacatgtt ccgcatcgtc gcctcgctca ggcgtcgcgc gcgaacgatt
      361 ccgcggaggt cgcccttgac gagggtcacg ccggcgcttt ccatcgctat gtccgtgccg
      421 gtgcccatcg cgatgccgac ttgagcctgc gcgagcgctg gtgcgtcgtt gatcccgtcg
      481 ccggccatcg ccacgaactt gccctccgac tgcagccgtt tgactacctg cgccttctga
      541 tcgggcagca cgtccgcgac gatctcgtcg atgccgagct gcgaggcgac ggcccgagcc
      601 gtcgtctcgt tgtcgccggt cagcatgacg atgcgcactc cggcgtcgtg cagcgccttg
      661 atcgcgggcg gcgtcgtgtc tttgatcgga tcggcgaccg ccaccaggcc ggccaggcgg
      721 ccgtcgacgg cgacgaacat cgcagttttt cccgcggcac gtagacgctc cacatgcgac
      781 gtcgacacgg tgacgtcgac gccgagcgtg ctcatgagcg cggcgttgcc gagcgcgact
      841 tgatgatcgt cgatctgccc acggacgcct ttgccgggcg ccgcttcgaa gccggtcacc
      901 tccttgagcg cgatgccacg ctcttgcgcc ccttgtacga tggccgtcgc gagcggatgc
      961 tcgctgccgc gctcgagcga agcggcgagc tggacgattg cggcatcggc gcgggcgtcc
     1021 agcgcgacga catcttggag caccgggcgc ccctgggtca gcgttccggt cttgtcgacg
     1081 acgagcgtgt cgacgcggcg caacgtctcg atcgcttcgg cgttcttgaa caggacgccg
     1141 aacg
//
LOCUS       DASUCJ010000557         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1442639, whole genome shotgun sequence.
ACCESSION   DASUCJ010000557 DASUCJ010000000
VERSION     DASUCJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442639"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(583..>1239)
                     /locus_tag="VFU90_06590"
     CDS             complement(583..>1239)
                     /locus_tag="VFU90_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU5479482.1"
                     /translation="DGKTASGDPTGNSGSGEGDVIGVDVANIKLGSGGGTYTGNALNN
                     VFYAGTGGASTVNGSTGDDTLSEGADANNGGSETFNGGAGTDTVDYSSRANPLSVTMD
                     GSTASGDTVNSEADVIGTDVENVYGGAGTDTIVGNDNDNDLEGNGGGDTICGGPGNDT
                     LLGNHAVGDSTAGDAAHLHGGDCTDDAEAGYNLCLNTGTDASATPAAAGSANCELVSH
                     "
BASE COUNT          177 a          396 c          440 g          226 t
ORIGIN      
        1 cgctcgagcc agaggagccg ctcatgccgc tcgtctcttg gctaccggcc ttgtgcgagc
       61 cggagcacgc tgcgagcgcc gcgctcgtcg tcagccagaa gcccagtcta ccgatcttca
      121 tttggaccca ttctcctcga cgctgttcgc tcccttcagt gcactcgcga tgccatcgtt
      181 cgagcggccc gtttctcgtg gctttcgcga atcgtcgggt gccgggtgat cgccgtcgcc
      241 cggcgatttg acgacgagct gcggcggatg cgaagaaggt gccgtcagcg cgatgacggt
      301 gcgcgtcagg catccgtcgg ctctccgccc gccgacaaaa agcgaaaacg acgggacgac
      361 accatgtcgc accgtcgctt cgcttgtttg cgggttggga gaaacagaaa cgcgagagag
      421 gggagaaaca gaaacgcgag agaggggaga aaccgaaacg cgagagaggg gagaaacaga
      481 aacgcgggtg ggagatgcgg atacgggcgg gcctcgatac gcgcgagaga aacgcggggt
      541 ggaggtgcgt aaaggggccc gtccggaggg ggatcgaatc actcagtgcg agacgagctc
      601 gcagttggcc gagccggccg ccgcgggcgt cgcgctcgcg tcggtgcccg tgttgaggca
      661 gaggttgtag ccggcttccg cgtcgtcggt gcagtcgccg ccgtggaggt gcgccgcgtc
      721 gcccgccgtc gagtccccca ccgcatggtt gccgagcagg gtatcgttgc cgggaccgcc
      781 gcagatcgtg tcgccgccgc cgttgccttc gaggtcgttg tcgttgtcgt tgccgacgat
      841 ggtgtccgtg ccggcgccgc cgtacacgtt ctcgacgtcc gtgccgatca cgtcggcctc
      901 gctgttgacg gtgtcgccgc tcgcggtgct gccgtccatc gtgacgctca gcgggttcgc
      961 gcggctgctg tagtcgaccg tgtccgtacc ggcgcccccg ttgaaggtct cgctgccgcc
     1021 gttgttcgca tcggcgcctt cgctgagcgt gtcgtcgccc gtgctgccgt tgaccgtcga
     1081 agcgccgccg gtaccggcgt agaagacgtt gttcagcgcg ttgccggtgt aggtgccgcc
     1141 gccgctgccg agcttgatgt tcgcgacgtc gacgccgatg acgtcgcctt cgcccgagcc
     1201 ggagttgccc gtggggtcgc cgctcgccgt cttgccgtc
//
LOCUS       DASUCJ010000558         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_953270, whole genome shotgun sequence.
ACCESSION   DASUCJ010000558 DASUCJ010000000
VERSION     DASUCJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953270"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFU90_06595"
     CDS             complement(<1..459)
                     /locus_tag="VFU90_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEU5479483.1"
                     /translation="MNVAVAVHARPRSKRVMLAAIVVVLASPPVAARAQSGGTTGDAW
                     QIVQPEQSSLVFARDGTSLIGEIGREIRTSVSIRSLPKYVGEAFISVEDQRFYQHDGV
                     DVVGVAAALKDALRGHARGASTITQQLVGNMHPDVINRSDRSIARKLREQA"
     gene            628..>1655
                     /locus_tag="VFU90_06600"
     CDS             628..>1655
                     /locus_tag="VFU90_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5479484.1"
                     /translation="MPSVIDVATVQFAPRKGEYEANLARLGELFAQLDGLTPRPQVLH
                     LPETALTGYFLEGGVREFALTAGAQAADLQRVYRNAVPQAADRTLEIVLGFYEVWNNI
                     LYNSAMWVRVGGAAPLVRHVHRKNFLPTYGLFDEERFVERGQSFRAFDTPWGRCAVLI
                     CEDAWHSLTGTLLALDGARAIFICSAAPARGPWPREDGIPGPASVARWERLVRDIAEE
                     HGVFVSFSNLASSEAGKSFPGGALVAGPKGDVRIRGPMWSEAILPIAMDLGDLTRARA
                     DMPLLADLRTAIPHLVGTLARIQYGHADALQFDSDGCEARTIPPATTSTARARSMDVV
                     DGGMAAAGT"
BASE COUNT          264 a          569 c          541 g          281 t
ORIGIN      
        1 cgcctgttcg cgcagcttgc gcgcgatgct ccggtcgctg cgattgatca cgtccggatg
       61 catgttgccg acgagctgct gcgtgatcgt gctcgcgccg cgcgcgtgcc ctcgcagcgc
      121 gtctttgagc gcggccgcga cgccgacgac gtccacgccg tcgtgctggt aaaagcgctg
      181 atcctcgacg gaaatgaacg cctcgccgac gtatttcggc agcgagcgga tcgacacgct
      241 cgtgcgaatc tcgcggccga tttcaccgat gagggacgtg ccgtcgcgcg caaacaccaa
      301 cgatgactgc tccggctgta cgatctgcca ggcgtcccca gtggtgccac cgctctgtgc
      361 gcgtgctgcg accggcggcg aagcgagcac aaccactatc gccgcgagca tcacccgctt
      421 cgaccgcggg cgcgcgtgca cggccactgc aacgttcata tcgttcacca aagctccagc
      481 caacccggcg caccatgcgc cggacgttcc caatgtgata cccgacagcg ttccgaatga
      541 cacacgatcc gcgggcggca agataacccg cccgtccccg aattctgtga cattgctcca
      601 ctgagcccgt cgcaccacct ttttgtcatg ccttccgtca tcgacgtcgc aaccgtccag
      661 ttcgcgcctc gcaagggcga atacgaggcg aacctcgcgc gcttgggcga gttgttcgcc
      721 caactcgacg gcctcacgcc ccggccgcag gtccttcacc tgcccgagac cgcgctcacc
      781 ggatatttcc tcgagggcgg cgttcgcgaa ttcgcgctca cggcgggcgc acaagcggcc
      841 gatctgcagc gcgtgtaccg caacgcggtc ccccaggcag ccgaccggac gctcgagatc
      901 gtgctcggct tttatgaggt ctggaacaac atcctctaca acagcgcgat gtgggtgcgg
      961 gtcggcggcg ccgcgccgct ggtcagacac gtccaccgca agaacttcct gccgacgtac
     1021 ggactgttcg acgaggaacg gttcgtcgag cgcggtcagt cgttccgggc tttcgacacg
     1081 ccgtggggtc gctgcgccgt actcatctgt gaggacgcct ggcacagcct gacggggacg
     1141 ctgctcgcac tggatggcgc ccgcgcaatt ttcatttgct cggcagctcc ggcgcgaggt
     1201 ccgtggcctc gggaggacgg aattcccggt ccggcaagcg tggcccgatg ggagcggctc
     1261 gtgcgcgaca tcgccgaaga gcacggggtg ttcgtgtcgt tctccaacct cgcatcgagc
     1321 gaggccggca aatcgtttcc ggggggcgcg ttggtcgcgg ggcccaaagg cgacgtgcgc
     1381 atccgtggtc cgatgtggtc ggaggcgatt ctgcccatcg cgatggatct gggcgatttg
     1441 acgcgtgccc gggccgacat gccgcttctg gccgacctcc gcaccgcgat accccacttg
     1501 gtcgggacgc tggcgcgaat ccagtacggc cacgccgatg ccctgcagtt cgacagcgac
     1561 ggctgcgagg cgcggacgat tccgccggcg acgacgagca cggcgcgggc gaggtcgatg
     1621 gacgtcgtgg acggcgggat ggccgcggcc ggcac
//
LOCUS       DASUCJ010000559         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1198140, whole genome shotgun sequence.
ACCESSION   DASUCJ010000559 DASUCJ010000000
VERSION     DASUCJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198140"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFU90_06605"
     CDS             <1..495
                     /locus_tag="VFU90_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5479485.1"
                     /translation="LAVTLVAFDAVVHTRGSAGSRDLPIANFFRLPGSTPQLETALEP
                     GALVTGVTVPLLPTGTRSTYLKVRDRAQYEFALASAAVAITIDNHTIREARIALGGVA
                     TIPWRAPEAERVLASSSPTRDNFEKAAAAALAGARGSGHNDFKIVLAQRTLVRALEIA
                     TAGT"
     gene            492..>1009
                     /locus_tag="VFU90_06610"
     CDS             492..>1009
                     /locus_tag="VFU90_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479486.1"
                     /translation="MTQPLIGKPLDRVDGPAKVTGAAKYTADHAFDGMLHAAVVTSTV
                     ARGRIVEIDDVQARAADGVVAVMTHANAPRVHPGKAMENHSILFLLQNDAVEFDGQPV
                     AVVLAETFEQATHAAGLVTVRYDAKPPHTDLLTGKLYVPEEVFGAAVTSQRGNPKAAY
                     DDAPVRLTHVYTT"
BASE COUNT          181 a          409 c          281 g          138 t
ORIGIN      
        1 ctcgcggtga cgctcgtcgc cttcgacgcc gtcgtccaca cgcgcggcag cgctggctcg
       61 cgcgacctgc cgatcgcaaa cttcttccgc ctccccggca gcacgccgca gctcgaaacc
      121 gcgctcgaac ccggcgcgct cgtcaccggc gtcaccgtcc cgctgctccc gaccggcacc
      181 cgctccacgt acctgaaggt gcgcgaccgc gcgcagtacg agttcgcact cgcttcggcg
      241 gccgtcgcca taacgatcga caaccacacc atccgcgaag cgcgcatcgc gctcggcggc
      301 gtcgccacca tcccgtggcg cgcacccgaa gccgaacgcg tgctcgcaag ctcctccccg
      361 acgcgcgaca atttcgaaaa agccgccgcc gccgccctcg ccggcgcgcg cggttccggc
      421 cacaacgatt tcaaaatcgt cctcgcgcaa cgcacgctcg tgcgcgcact cgaaatcgca
      481 acggccggga catgacccaa cccctcatcg gcaaaccgct cgaccgcgtc gacggccccg
      541 ccaaagtcac cggcgccgcc aagtataccg ccgaccacgc gttcgacggc atgctgcacg
      601 ccgccgtcgt aaccagcacc gtcgcccgcg gccgcatcgt tgaaattgac gacgtgcaag
      661 cgcgcgccgc cgacggcgtc gtcgccgtca tgacgcacgc caacgccccg cgcgtgcatc
      721 ccggaaaggc gatggagaat cattcgattc tctttctgct gcagaacgac gcggtcgagt
      781 tcgacggcca gccggtcgcg gtcgtcctcg ccgaaacgtt cgaacaagcc actcacgccg
      841 ccggtctcgt gaccgtgcgc tacgacgcga agccgccgca taccgatctg ctcaccggca
      901 aactttacgt gcccgaagaa gtcttcggtg ccgccgtgac cagtcaacgc ggcaacccca
      961 aagccgctta cgacgacgcc ccggtgcggc tgacgcacgt ctacacgac
//
LOCUS       DASUCJ010000560         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1167523, whole genome shotgun sequence.
ACCESSION   DASUCJ010000560 DASUCJ010000000
VERSION     DASUCJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167523"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..>1105)
                     /locus_tag="VFU90_06615"
     CDS             complement(59..>1105)
                     /locus_tag="VFU90_06615"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5479487.1"
                     /translation="SPRSFSYMPMTTANKNAAGNGAASKNGDVTHSVTPGERRLLTAL
                     RAFRSGDFSVRLPVKGSGAEAEIAQAFNEVLETKTRLLGEIKRVSRSISRDGRLSLRA
                     QLEGASGGWQEHVEAYNSLIETVAAPMAETNRVLAAVAQGDLSQRMTVEIDGHPLRGD
                     FLKSAKVINAMVDQLNAFASEVTRVAREVGSEGKLGGQASVKGVAGTWKDLTDSVNTM
                     ASNLTSQVRNIADVTTAVAKGDLSRKITVEARGEILELKNTINTMVDQLNSFAGEVTR
                     VAREVGSEGKLGGQAEVAGVSGTWKDLTESVNFRASNLTSQVRNIAEVTTAVARGDLS
                     RKISVDVQGEILEL"
BASE COUNT          179 a          352 c          334 g          240 t
ORIGIN      
        1 cgcgcgtcac ttccgacgcg aaggcgttca gctggtccac catcgtgttg atcgtgttct
       61 acaactcgag gatttcaccc tggacgtcga ccgagatctt gcgcgagaga tcgccgcgcg
      121 cgacggccgt cgtgacttcg gcgatgttac gaacctgaga cgtcaggttc gaagccctga
      181 agttcacgga ttccgtcaag tctttccacg tgccggaaac gcccgcaacc tctgcttgtc
      241 cgcccagttt gccttccgag cccacttcgc gcgcgacgcg cgtgacttcg cctgcgaacg
      301 agttgagctg gtcgaccatc gtgttgatcg tgttcttcaa ctcgagaatc tcgccgcgcg
      361 cctcaacggt gatcttacgg gagaggtcgc cctttgccac ggccgtcgtc acgtcggcga
      421 tgttacgcac ctgcgaggtg aggttcgatg ccatcgtgtt gacggagtcc gtcaagtctt
      481 tccacgtgcc tgcgacgcct ttgacactcg cctgaccgcc gagtttgcct tcgcttccga
      541 cttcgcgagc gacgcgcgtg acctccgacg cgaacgcgtt cagctggtcg accatcgcgt
      601 tgatgacctt tgccgatttc aagaagtcgc cgcgcagcgg atgtccgtcg atctcgacgg
      661 tcatgcgctg ggagagatcg ccctgcgcca cagccgcgag aacgcggttg gtttcggcca
      721 tcggcgccgc aacggtttcg atgagggagt tgtacgcctc gacgtgctcc tgccatccgc
      781 cgctcgcgcc ttccagctgc gcgcgcagcg agaggcggcc gtcgcgcgag atcgaacggc
      841 tgacgcgctt gatctcgccg agcagacgcg tcttcgtttc caacacttcg ttgaaagctt
      901 gcgcgatctc ggcttcggct ccgctgccct tgaccgggag gcgtacgctg aagtcgccgc
      961 ttctaaatgc gcgcagcgcc gtcagtagac ggcgttcgcc gggggtaacg gaatgcgtaa
     1021 cgtcgccgtt tttagacgca gcgccgttac cagcggcatt tttattggcc gtggtcatag
     1081 gcatgtaaga aaagctccgt gggct
//
LOCUS       DASUCJ010000561         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_464528, whole genome shotgun sequence.
ACCESSION   DASUCJ010000561 DASUCJ010000000
VERSION     DASUCJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464528"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="lnt"
                     /locus_tag="VFU90_06620"
     CDS             <1..837
                     /gene="lnt"
                     /locus_tag="VFU90_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU5479488.1"
                     /translation="EVAAVQGNIAQSLKWNSLELAVLRYETMTRAAAARRPKLIVWPE
                     TVIPTVLDRDPILTNRFAGLAVASHATLVVGSESEHGRRLYNSLFVFQPDGTYATYDK
                     RQLVPFAESFPGRRVLSWLPYVGELSGRFGTGRVDAVYPTAALSIAPLICWESAFSDL
                     SYAQIRRGAQLLVVSTDDAWFGTTSGPYMHAQIAQLRAVESGAYVVRAAATGISGVIA
                     PDGSWQRRSRMEEQVVVTGKVGPRAATFFASIGPVPVALALAALYVAILLGGRKRGDV
                     AG"
     gene            824..1408
                     /locus_tag="VFU90_06625"
     CDS             824..1408
                     /locus_tag="VFU90_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479489.1"
                     /translation="MSPASKRTWRRVAIVAGVALIVWVVVEVILAGQGAPPPPPSSIS
                     MDLRGGHVLNNRISTKSWTFDYDHARLSPDGFSGSVDGVRNGIVFRKGKPYLRISAEH
                     IQLDIQSLDFTAIGKVHVERIDDPQKRAFDTDLVTWTNDAKLLRMTHPAYVHTNGQTL
                     RIDGITVDFDADTIHLGKVGGQVDLHKERAVPAP"
BASE COUNT          260 a          521 c          512 g          298 t
ORIGIN      
        1 gaagtggccg cggttcaggg aaacatcgcg cagtcgctca agtggaactc gctcgagctg
       61 gcggtcctgc ggtacgaaac gatgacgcgc gccgccgccg cgcgcaggcc gaagctcatc
      121 gtctggccgg agaccgtcat cccgacggtc ctcgatcgcg atccgatcct gacgaaccgg
      181 tttgccggcc tcgcggtcgc atcgcacgcc acgctcgtcg tcggaagcga aagcgagcac
      241 ggccgccggc tgtacaattc gctgttcgtg tttcagcccg acggaacgta cgcgacctac
      301 gacaaacgcc agctggtgcc gtttgccgaa tcgtttccgg gcaggcgcgt gctctcgtgg
      361 ctgccctacg tgggtgagct gagcggccgc ttcggaacgg ggcgcgtcga cgccgtctat
      421 ccgaccgccg ccttgagtat cgcgccgctg atctgctggg aatccgcctt ttcggatctg
      481 agctacgcgc agatccggcg cggcgcgcaa ctgctggtcg tcagcaccga cgacgcctgg
      541 ttcgggacga cgtcggggcc ttacatgcac gcgcagatcg cgcagctgcg ggcggtcgaa
      601 agcggagcct acgtcgtgcg cgcggccgcg accggaatca gcggggtgat cgcaccggac
      661 ggcagctggc agcggcgcag ccgcatggaa gagcaagtcg tcgtcaccgg aaaggtcggg
      721 ccgcgggcgg cgacgttctt cgcgtcgatc ggaccggtgc cggttgcgct cgcgctcgcg
      781 gcgctctacg ttgcgattct gctcggcggc cgaaagcgcg gcgatgtcgc cggctagcaa
      841 acgcacctgg cgtcgcgtcg cgatcgtcgc gggcgtcgca ttgatcgtct gggtggtggt
      901 cgaagtgatc ctcgccgggc agggcgcgcc cccgccgccc ccgtcgtcga tctcgatgga
      961 tttgcgcgga ggccacgttc tgaacaatcg catctcgacc aaatcgtgga cgttcgacta
     1021 cgatcacgcg cgcctctcgc ccgacgggtt ctccggcagc gtcgacggcg tgcgcaacgg
     1081 catcgtcttt cgcaagggaa agccgtatct gcgcatctcc gccgagcaca ttcagctcga
     1141 catccagtcg ctggatttta ccgccatcgg caaggtgcac gtggaacgca tcgacgaccc
     1201 gcaaaagcgc gcgttcgaca ccgatctcgt cacctggacg aacgacgcca agctgctgcg
     1261 tatgacgcat ccggcgtacg tgcacacgaa cggtcagacg ttgcgcatcg acggaatcac
     1321 cgtcgacttc gacgccgaca cgatccatct cgggaaggtg ggggggcagg tagacctgca
     1381 caaagaacgc gcggtgccgg ccccgtaatc ggggccggct tttcgctatg cgttgccttt
     1441 ttcgtcgcga agtttctttt tggcctcggc gatcagtttc ttgaccttct gacctccctt
     1501 atggccgatt tcctcgtaaa agtcggagcc gtatttttct ttgacgacct ttccgccttt
     1561 ttgcccgatg gactcgtaga actccacgcc g
//
LOCUS       DASUCJ010000562         7541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_234962, whole genome shotgun sequence.
ACCESSION   DASUCJ010000562 DASUCJ010000000
VERSION     DASUCJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234962"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFU90_06630"
     CDS             complement(<1..211)
                     /locus_tag="VFU90_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479490.1"
                     /translation="MAEGTLSFAADVRPMFTDLDVDHMQPLGIDLSDRAAVEANADAI
                     YAAVSNGSMPPAGTGERWSPEMCARF"
     gene            365..2044
                     /locus_tag="VFU90_06635"
     CDS             365..2044
                     /locus_tag="VFU90_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479491.1"
                     /translation="MATLLAVLRGALCAGLLGLLGDLAPAAAAEPPVPPPSAPPGTVA
                     QTLPSATPTPDPFKWSGTFRSYYFTRQNASNNPGAQFNFTPGAKYSTTGVNQATWNSS
                     IGLHGEYAIPDTGGVFVGGSYLYANPLDGPCSVAYPEHIKGQPCVSQTPPNTNPDDTL
                     PGFTLDTFYEAYLGYRDRSWYAKVGDQLFTSPWANPADTRLKPEAFQGADVVFSGVKN
                     WTFETADMIQFENRTSNAFQSNTLLTSYPAGNSGLPANIYVPGGNFIQTNGFFYAKAG
                     YANASTGLSLDGYFYSVSALMNILWFDGKYAFNDSRLEPFIELQGGTESNAGASYLGK
                     IDSQVFGVRVGANVTKNVVLTFGLDTLPWHNDTIVLPHGASCSSATNQITAKGVTVPY
                     FLPLVGNGTGQCANNADGTTTIYYGGWASPYTDNYTADPFFTTNGSQGMVERRSAGTS
                     ERVALTYTSSNNRIVFLAAYGWLNYGNGAAPSANTNEYDLDGIYRFSPYKGTGPYRGL
                     MLRDRYFVRSIDNTFCGAAATTCPAGSAYGAQYLGGLPLFKYNRAMLEYDF"
     gene            2103..3152
                     /gene="tdh"
                     /locus_tag="VFU90_06640"
     CDS             2103..3152
                     /gene="tdh"
                     /locus_tag="VFU90_06640"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5479492.1"
                     /translation="MKALSKTAAGRGFTLTDVAVPEIGPTDVLIRVEKAGVCGTDLHI
                     YEWDKWAQARIRPPMVVGHEFMGTIAAVGDAVRSVRIGDRVSAEGHIADLTCYLCRTG
                     QAHICERVQIIGVDRDGAFAEYVAMPEYNVWKLDPAIPDEYAAIFDPLGNAVHTVMAA
                     GVSVKSVAITGVGSIGLMAIPVARAAGAASVFAIDVNPGKLELAKRLGADEAFSATQP
                     GLIDEIVARTNGDGVDVLLEMSGSGAAIDAGLQMVRNGGTAALLGIPSDSVNINLAER
                     IIFKGLTVLGINGRRMFETWYQTQALVKSGRIDLRPIITHVLPYAQFDRAFELMRSGE
                     AAKIVLDFTGDIAKP"
     gene            3149..4372
                     /locus_tag="VFU90_06645"
     CDS             3149..4372
                     /locus_tag="VFU90_06645"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU5479493.1"
                     /translation="MNRAFEEKLRGDLENLKRAGTYKHLRHITTPMAPEVHMEEAGDV
                     IVLSSNNYLGLSDQPEVVEAGKRGLDRYGAGTASVRFICGTFDIHRTLEERIANFLGT
                     EAALSYVSCWNANTGLFATICDEGSAIVSDELNHASIIDGVRLASKARRERYKHSDMR
                     ELAAKLEAVAGCFPIVIVTDGVFSMEGDLAKLPEIVELARQHGAIVVVDDSHGTGVMG
                     KTGRGTIEHYGLTGQIDVITGTLGKALGGAAGGFVAGSGALIDTLIQRSRPQLFSNAL
                     PATVACSALAAIDYLDAHPETIDRLRANTKYFREGLERIGYRPLPGESAIVPIIVGET
                     AFAIAMSDRLLKRGVFVTGFGFPVVPEGTARIRVQISAALTKDEMDRALAAFKSAGEE
                     AGLLKDGAAATGSAG"
     gene            complement(4353..4499)
                     /locus_tag="VFU90_06650"
     CDS             complement(4353..4499)
                     /locus_tag="VFU90_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479494.1"
                     /translation="MTGLTIFRSLNEALQRGYQVYDRTPEGYLVRIRTERGWALALVI
                     PQTP"
     gene            4612..5130
                     /locus_tag="VFU90_06655"
     CDS             4612..5130
                     /locus_tag="VFU90_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479495.1"
                     /translation="MGGSGLILQIFLVTAVLAIGVMLFLANAGAGAPLPQEAVTARGY
                     RLRRYWFWIVFAGAIAAFVLTIPHFPYPRAAASAGVKHVRVVAQQYGFTVDAVVPLRQ
                     PVVFDVTSKDVNHGFGIYAPDGSLVSQVQAMPEYVNHLPVTFNVPGHYTIRCLEYCGI
                     AHAAMQAGFDVR"
     gene            5127..6590
                     /locus_tag="VFU90_06660"
     CDS             5127..6590
                     /locus_tag="VFU90_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5479496.1"
                     /translation="MTALPSAPGTRVMWTYIATGLAIFALMLLAGIGLRASQAGWLAV
                     DPGTFYSILSLHGVGMIVAMAIAGLGLLWYLVRRETELDERPAWIAYGFFVAGVACAI
                     VAVVPGRYGSLWTMLYPLPFVGTYWPSWATGAWLIGNALVMLGFMVWCGQILGALLQR
                     YGGLRGMLAIDYVLRNREFVQRGGQPPPPQMFAAAVTAFDGFITGTVGLVIGTALIVH
                     WIDPSVTIDPLWAKNLTYLFGHSFANLTMYMAVAALYVALPLCVNRPYKTSPQLAAAW
                     WGTLLFVMIAYFHHLYMDFVQMRWLQYVGEIASYLAAIPVVVVTVYGGVMLVHRSGLR
                     WSLGAMFLYAGMIGWVVGGIAAVLDATIPANLDLHNTLWVPGHFHTYLLEGVLLFLLG
                     WVFVNLEQRADGRSSLLTRWLVGIGVFGGGALFLLSFYVAGAAGVPRRYALEPAPGPL
                     WSGLATIGAIVLLAGLALAAFEGVRLARARAAAVTAP"
     gene            6587..7306
                     /locus_tag="VFU90_06665"
     CDS             6587..7306
                     /locus_tag="VFU90_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5479497.1"
                     /translation="MNGTVRVIAFVVLAAVVLFALAPLVLGAEEIPTARHHFLHAVML
                     AAAAFAGILLAGRSRRAGNAGWLVVAMVSPVIAMLLMWPSEYAYFETHRYGHVLEHLA
                     LIGCGFLTGYGGQRYADGIGWAAGLSVVAMALIGIWGYGVGPASATAPVSQTVQPPVF
                     AVPDVAHGATVFKQNCAVCHGVDGRGAEAPSLRNESSRKRLAAAEAWILHPAPPMPAL
                     YPDRLSAQDVADVAGYIETLR"
     gene            complement(7320..>7541)
                     /locus_tag="VFU90_06670"
     CDS             complement(7320..>7541)
                     /locus_tag="VFU90_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479498.1"
                     /translation="KERRFGTMWCENADPTLAALRALPNGLRGRCAGCRYAGICGGNF
                     RARALNATGDLWGSDPACYLSDDEVATSP"
BASE COUNT         1274 a         2404 c         2521 g         1342 t
ORIGIN      
        1 tgaaccgcgc gcacatttcc ggcgaccatc gctcgcccgt acccgccggc ggcatgctgc
       61 cgttgctgac cgccgcatag attgcgtcgg cgttggcctc gacggcggcc cggtcggaga
      121 ggtcgatacc gagcggctgc atatgatcga cgtcgaggtc ggtgaacatc ggccgcacgt
      181 cggcggcaaa actcaaggtg ccttcagcca tcactgaaat ctcctaataa cgggagtcta
      241 ctacgggact ctacccgttc gagcgagctc cgccccgttg gctgaaaagg atgacctagg
      301 ttattttcgc tggttgtaaa aagagatgct agtcaacttt tctgcaaagg agactcctct
      361 ctcaatggct acactgctcg cagtcctccg gggagcgctg tgcgccggcc tcttagggct
      421 cctaggggac ctcgcaccgg ccgccgcggc cgaaccgccg gtcccgccgc cgtcggcgcc
      481 gccggggacg gtggcgcaaa ccctcccgtc cgctacgccc acccccgatc cgttcaagtg
      541 gagcgggacg tttcgttcgt actactttac gcgccaaaac gcttcgaata atcccggcgc
      601 acaattcaac ttcacaccgg gtgcgaagta ctccaccacc ggcgtcaacc aggcgacgtg
      661 gaacagctcg atcggtctgc acggcgagta tgcaataccg gacacgggcg gcgtgttcgt
      721 cggcggatcg tacctctacg ccaacccgct cgacggcccg tgttcggtcg cctaccccga
      781 gcacatcaaa gggcagccgt gcgtgagcca aacgccgccc aatacgaatc ccgacgacac
      841 gcttcccggc ttcacgctgg acacgttcta cgaagcgtat ttgggatatc gcgaccgcag
      901 ctggtacgcg aaggtgggcg atcagctgtt cacgtcgccg tgggcgaatc cggccgacac
      961 gcggctcaag ccggaggctt ttcagggcgc cgacgtcgtc ttctcgggcg tgaagaactg
     1021 gacgttcgaa acggccgaca tgattcagtt cgagaaccgc acgagcaacg cgtttcaaag
     1081 caacacgctg ctgacgagct atccggccgg caactccggc ttacccgcca acatctacgt
     1141 tccgggcggc aacttcatcc aaaccaacgg attcttctac gccaaagccg gctacgccaa
     1201 cgcttcgacc gggctgagcc tggacggcta cttctacagc gtgagcgcgt tgatgaacat
     1261 tctctggttc gacggcaagt acgcgttcaa cgatagccgt ctcgaaccgt tcatcgagct
     1321 gcagggcggc accgaaagca acgccggcgc ctcgtacctc gggaagatcg acagccaagt
     1381 tttcggcgta cgggtcggag ccaacgtgac gaaaaacgtc gtgctgacct ttgggctcga
     1441 cacgcttcca tggcacaacg acacgatcgt gctgccgcac ggtgcgagct gcagcagcgc
     1501 taccaatcaa atcaccgcca aaggcgtgac cgtcccgtac ttcttgccgc tagtcggcaa
     1561 cggtaccggt cagtgcgcca acaacgccga cggaacgacg acgatctact acggcggctg
     1621 ggcgagcccg tacaccgaca actacaccgc cgacccgttc ttcacgacca acggatcgca
     1681 aggaatggtc gagcgccgct ccgcggggac ctcggagcgg gtggcgctca cctacacgtc
     1741 gagcaacaac cgcatcgtgt ttttggcggc gtacggctgg ctcaactacg gcaacggcgc
     1801 ggcaccgtcg gcgaacacga acgaatacga cctcgacggc atctaccgct tcagtccgta
     1861 caaaggcacc ggaccgtatc gcgggctgat gctgcgcgat cgctacttcg tgcgcagcat
     1921 cgacaacacg ttctgcggag cggccgcgac gacgtgcccg gccggctcgg cgtacggcgc
     1981 gcaatatctc ggcggcttgc cgctgttcaa gtacaatcgc gcgatgctcg aatacgactt
     2041 ctagccggca gcaggatgac aggggcggct cggggtgcaa tacttcgggc cgcatgccct
     2101 ccatgaaagc actctcgaaa accgccgccg gacggggctt tacgttgacc gacgtcgcgg
     2161 tgcccgagat cggcccgacc gacgtgctca tccgggtcga gaaagccggc gtctgcggca
     2221 cggacctgca catctacgaa tgggacaagt gggcgcaggc acggattcgg ccgccgatgg
     2281 tcgtcgggca cgagttcatg gggacgatcg cggccgtcgg cgacgccgtg cgctcggtgc
     2341 ggatcggcga ccgcgtttcg gcggaggggc acatcgccga cctcacgtgt tacctgtgcc
     2401 gcaccgggca agcgcacatc tgcgaacgcg tgcagatcat cggcgtcgat cgcgacggcg
     2461 catttgccga gtacgtggcg atgccggagt acaacgtctg gaagctcgat cccgcgatcc
     2521 ccgacgagta cgccgcgatc ttcgatccgc tcggcaacgc cgttcacacg gtgatggcgg
     2581 ccggcgttag cgtcaagagc gtcgcgatca cgggcgtcgg ctcgatcggg ctgatggcga
     2641 ttccggtggc gcgcgccgcc ggcgcggcct cggtgttcgc catcgacgtc aatccgggaa
     2701 aattggaact ggccaagcga ttgggcgccg acgaggcgtt ttcggcgacc cagccggggc
     2761 tgatcgacga gatcgtggcg cgaaccaacg gcgacggcgt cgacgtgctg ctggagatgt
     2821 cgggcagcgg cgcggcgatc gacgcgggct tgcaaatggt gcgcaacggc ggtaccgcgg
     2881 cgctgctcgg catcccgagc gacagcgtca acatcaacct ggccgaacgc atcatcttca
     2941 aagggctgac ggtgctgggc atcaacgggc ggcggatgtt cgagacgtgg tatcagacgc
     3001 aggcgctggt caagagcggg cggatcgact tgcggccgat catcacgcac gtgctgccgt
     3061 acgcgcagtt cgaccgcgcg ttcgagctga tgcgcagcgg cgaagcggcc aagatcgtat
     3121 tggacttcac gggggacatc gcaaaaccat gaatcgagct ttcgaggaaa aactgcgcgg
     3181 cgatctcgag aacctcaaac gcgcgggcac gtacaaacat ctgcgccaca tcacgacgcc
     3241 gatggcgccg gaagtccaca tggaagaagc cggcgacgtg atcgtgctct cgagcaacaa
     3301 ttacctcggc ctttccgacc agccggaagt ggtggaagcc ggcaaacgcg ggctcgaccg
     3361 gtacggcgcg gggacggcct cggtgcgatt catctgcggc acgttcgaca tccaccgcac
     3421 gctcgaggaa cgcatcgcca atttcctcgg aactgaggcg gcgctgtcgt acgtctcgtg
     3481 ctggaacgcc aacaccgggt tgttcgcgac gatttgcgac gagggctcgg cgatcgtttc
     3541 cgacgagctg aaccacgcgt cgatcatcga cggggtgcgg ctggcctcga aggcgcggcg
     3601 cgagcgttac aagcacagcg acatgcgcga gctggcggcg aagctcgagg cggtcgcggg
     3661 ctgcttcccg atcgtgatcg tgaccgacgg agtattttcg atggagggcg atctcgcgaa
     3721 actacccgag atcgtcgagc tggcgcgcca gcacggggcg atcgtggtcg tcgacgactc
     3781 gcacgggacc ggcgtgatgg ggaagaccgg gcgcggaacg atcgagcatt acggcttgac
     3841 cgggcagatc gacgtcatca ccggtacgct cggaaaggcg ctcggcgggg cggccggcgg
     3901 attcgtggcg ggcagcggcg cgttgatcga tacgctgatc cagcgttcgc gcccgcagct
     3961 cttttcgaac gcgctgcccg cgacggtggc gtgcagtgcg ctggcggcga tcgactatct
     4021 cgacgcacac cccgaaacga tcgaccgcct ccgagcgaac acgaagtact tccgcgaggg
     4081 gctcgaacgg atcggctatc gcccgctgcc gggggaaagc gcgatcgtgc cgatcatcgt
     4141 cggcgaaacg gcgttcgcaa tcgcgatgag cgaccggctg ctcaaacgcg gcgttttcgt
     4201 gacggggttc ggattcccgg tcgtgcccga ggggacggcg cgcatccgcg tgcagatcag
     4261 cgcggcgctg acgaaggacg agatggaccg cgcgctggcg gcgttcaagt cggccggcga
     4321 agaagccggc ctgttgaaag acggcgcggc ggctacgggg tctgcgggat aaccagcgcc
     4381 agcgcccagc cccgctcggt acggatccgc acgagatagc cttcgggggt ccggtcgtag
     4441 acttggtacc cgcgctgcag tgcctcgttc agagacctga atatcgtcaa accagtcatc
     4501 gtcctcgtcc tccagctgcc tcctcatcga ccgtgcatcc aacatacccc aaaaatgtga
     4561 agaatgtttt gaagtccggt taaagaacgc cgtagtccgg cggaggtcaa gttgggcggc
     4621 agcggtctaa ttcttcaaat cttcctcgtc acggcggtcc tggcgatcgg cgtcatgctg
     4681 ttccttgcca acgcgggggc aggcgcgccg ctcccgcagg aggccgtgac cgcgcgggga
     4741 taccggctgc ggcggtactg gttttggatc gtttttgccg gcgcgatcgc ggcttttgtc
     4801 ttgacgattc cgcactttcc ctatccgcgg gcggcggcgt cggccggcgt caaacacgtg
     4861 cgcgtcgtcg cccagcagta cggcttcacg gtcgacgcgg tggtgccgct gcggcaaccc
     4921 gtcgttttcg acgtcacttc gaaggacgtc aatcacggct tcggcatcta cgcacccgac
     4981 ggttcgctgg tcagccaggt gcaagcgatg cccgagtacg tcaatcatct gccggtgacg
     5041 tttaacgtgc cggggcacta cacgatccgc tgcttggaat actgcggcat cgcgcacgcc
     5101 gccatgcagg cggggttcga cgtccgatga ccgcgttgcc gagcgcgccc ggcacgcgcg
     5161 tgatgtggac gtacatcgcg accggactcg caatcttcgc gctcatgctg ctcgcgggga
     5221 tcggtttgcg cgcgtcccaa gccggctggc tggccgtcga tcccggtacc ttttactcga
     5281 tcttgagcct gcacggcgtc gggatgatcg tcgcgatggc gatcgccggc ctcgggctgc
     5341 tgtggtatct cgtacggcgc gagacggagc tcgacgaacg tccggcgtgg atcgcctacg
     5401 gatttttcgt cgccggcgtc gcgtgcgcga tcgtcgcggt cgttccgggg cgatacggat
     5461 cgctgtggac gatgctctat ccgctgccgt tcgtgggaac gtactggccg agctgggcga
     5521 ccggcgcgtg gctgatcggc aacgcgctgg tgatgctcgg attcatggtg tggtgcggcc
     5581 agattctcgg cgcgctgctg cagcgctacg gcggactgcg cggaatgctc gcgatcgatt
     5641 acgtgctgcg caaccgcgag ttcgtgcagc gcggcgggca gccgcccccg ccgcagatgt
     5701 tcgccgccgc ggtcacggcc ttcgatgggt tcatcaccgg aacggtcggg ctggtcatcg
     5761 gcacggcgct gatcgtgcac tggatcgatc catcggtaac gatcgatccg ctctgggcga
     5821 aaaatctgac gtacctcttc gggcattcgt tcgcgaatct cacgatgtac atggcggtag
     5881 cggcgctcta cgtcgccctg ccgctgtgcg tcaaccgtcc ctacaaaacg tcgccgcagc
     5941 tggcggccgc gtggtgggga acgctgctgt tcgtcatgat cgcctacttc caccatctct
     6001 acatggattt cgtccagatg cgctggctgc agtacgtggg tgagatcgct tcgtatttgg
     6061 cggcgattcc ggtggtggtg gtgaccgtgt acggcggcgt gatgctggtg catcgttccg
     6121 ggctgcgctg gtcgctgggc gcgatgttcc tgtacgccgg catgatcggc tgggtggtcg
     6181 gcgggattgc ggccgtgctc gacgcgacca ttcccgccaa cctcgatctg cacaacacgc
     6241 tatgggtgcc gggacacttc cacacgtact tgctggaagg cgtgctgctg ttcttgctcg
     6301 gctgggtgtt cgtgaacttg gaacagcgcg cggacggccg ctcttcgctg ctgacgcgct
     6361 ggctggtcgg gatcggggtg ttcggcggcg gcgcgctgtt cttgctgtcg ttctacgtcg
     6421 cgggtgcggc cggcgttccg cgccgctacg cgctggagcc ggcgccgggt ccgctgtggt
     6481 cgggcctcgc aacgatcggc gcgatcgtac tgctcgccgg gctcgcgctg gcggcgttcg
     6541 aaggggttcg gctggcacgc gcgcgcgctg ccgcggtgac ggcgccgtga atggaaccgt
     6601 gcgcgtcatt gcgttcgtcg tgctggcggc ggtcgtactg ttcgcgctgg cgccgctggt
     6661 gctgggcgcg gaagaaatcc ccacggcgcg ccatcatttt ctgcacgccg tgatgctcgc
     6721 cgccgccgcg ttcgccggca ttctgctggc cggccgcagc cggcgcgcgg ggaacgcggg
     6781 gtggctggtg gtcgcgatgg tttcgccggt gatcgcgatg ctgctgatgt ggccttcgga
     6841 gtacgcgtat ttcgaaacgc atcgctacgg ccacgtgctc gaacatttgg cgttgatcgg
     6901 ctgcggattt cttaccggct acggcgggca gcgctatgcc gacgggatcg gctgggcggc
     6961 cggattgagc gtggtcgcga tggcgctgat cggcatttgg ggctacggcg tcggcccggc
     7021 gagcgcgacc gctccggtat cgcaaacggt gcagccgccg gtattcgccg tgcccgacgt
     7081 cgcgcacggc gcgaccgttt tcaaacaaaa ctgcgcggtg tgccacggcg tagacggacg
     7141 cggcgccgag gcgccctcgc tgcgcaacga gtcgtcgcgc aagcggctgg ccgcggccga
     7201 agcgtggatc ttacatccag cgccgccgat gccggcgctc tatcccgacc ggctgtcggc
     7261 acaagacgtc gccgacgtcg ccggctacat cgaaacgctg cgctaggcgt tacgcggcgc
     7321 tacgggctgg tcgcgacctc gtcgtcgctc agatagcacg ccggatccga accccagaga
     7381 tcgccggtag cgttgagcgc gcgagcgcgg aagttcccgc cgcagatgcc ggcgtaccgg
     7441 caacccgcgc agcggccgcg taaaccgttg ggcaacgcgc gcagcgcggc gagggtcggg
     7501 tcggcgtttt cgcaccacat cgtgccgaag cggcgctctt t
//
LOCUS       DASUCJ010000563         1371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_250460, whole genome shotgun sequence.
ACCESSION   DASUCJ010000563 DASUCJ010000000
VERSION     DASUCJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250460"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VFU90_06675"
     CDS             <1..1251
                     /locus_tag="VFU90_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479499.1"
                     /translation="AYKDPNGWDMAHPVGTGPYRLADWRRGQRIVLEANPGFRDERYP
                     EGGDAADRELARGLAGRRLPLAKRVEISIIEEAQPRMLEFRRGNLDYVAVPNDLISKV
                     LLPDGKLAPDLAKAGVRMAHGVQPAIAYVYFNMDDPVLGGYKPAQIALRRAVAMAYDV
                     NEEIRVLREGQGFPATQVVPPNMTGHDPDYDGRISYDPAAAKALLERFGYVDRNGDGF
                     RDQPDGAPLVLKIGSDTASDSRQYDELWKKSLNAVGLRVEFVKQKWPDLLKAARLGQL
                     PMWYLGNINTTPDGFGFFGLLYGKNAGFSNLSRFALPEFDRLYEEARAMPNGPERLAV
                     ERKMSELVRAYAPWILTAFRYETILLQPWLRGYKYNPTYQMPFQYLDVGPRYGKAPTA
                     SQIDADAGRAAPAAGAAGMDRGAH"
BASE COUNT          233 a          466 c          468 g          204 t
ORIGIN      
        1 gcgtacaagg atcccaacgg ctgggacatg gcgcacccgg tcggcaccgg gccgtaccgg
       61 ctcgcggact ggcgccgcgg ccagcggatc gtgctcgaag cgaatcccgg atttcgggac
      121 gaacgctacc ccgagggcgg cgacgccgcc gaccgcgagc tcgcgcgagg gctcgcggga
      181 cgccgcttgc cgctcgcgaa acgcgtcgag atcagcatca tcgaggaagc gcagccgcgg
      241 atgctcgaat tccggcgcgg caatctcgac tacgtcgcgg taccgaacga cctcatcagc
      301 aaggtgctgc tccctgacgg caagctcgcg cccgacctcg cgaaggcagg agtgcgcatg
      361 gcgcacggcg tgcagccggc gatcgcctac gtgtacttca acatggacga tccggtgctg
      421 ggtggctaca agcccgcgca gattgccctg cgccgcgcgg tcgcgatggc ctacgacgtc
      481 aacgaggaaa ttcgcgtgct gcgcgaaggg cagggcttcc ccgcgacgca ggtggtgccg
      541 cccaacatga ccgggcacga tcccgactac gacggcagga tttcctacga tccggcggcg
      601 gcgaaagcgc tcctcgagcg cttcggctac gtcgatcgca acggcgacgg ttttcgcgac
      661 cagcccgatg gcgctccgct cgtgctgaag atcggctccg acaccgcttc ggacagccgc
      721 cagtacgacg agctatggaa gaagtcgttg aacgcggtcg ggctgcgcgt cgagttcgtc
      781 aagcagaagt ggccggatct cctgaaggcg gcacggctcg ggcagctccc gatgtggtac
      841 ctcggcaaca tcaacacgac acccgacggc ttcggcttct tcggtcttct ctacgggaag
      901 aatgccggct tctccaacct gtcgcgcttc gcgctgcccg aattcgaccg cctgtacgaa
      961 gaggcgcgcg caatgccgaa cggtcccgag cggctcgcgg tcgagcggaa gatgtccgag
     1021 ctcgtgcgcg cgtatgcgcc gtggatcctc accgcgtttc gctacgagac gatcctgctg
     1081 cagccttggc tgcgcggcta caagtacaac ccgacgtatc agatgccgtt ccagtacctc
     1141 gacgtcgggc cgcggtacgg caaggcaccg acagcgtcgc agatcgatgc cgacgcgggg
     1201 cgtgcggcgc ctgcagccgg agctgcggga atggatcgtg gcgcgcactg acgatcggcc
     1261 cgatcggccg aaccgcccgc ggcgccgcgt ggttgcggca ctcgtcgcgc tggcagcgct
     1321 gccttccgtc gcggcgacgg cgcagccgcg cggcggcaag gacagggcgt c
//
LOCUS       DASUCJ010000564         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1198193, whole genome shotgun sequence.
ACCESSION   DASUCJ010000564 DASUCJ010000000
VERSION     DASUCJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198193"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFU90_06680"
     CDS             <1..936
                     /locus_tag="VFU90_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU5479500.1"
                     /translation="VTFETATQARKLADALGGKAHAVVLGSGAAALADQLKAYPLDTI
                     HVNDDSDVDKFLLDPVVDYLEAVARATGPALVLVPNTLSGRDVAGRLVARLGAGLGAD
                     VTDFTVANGSVECVSPKMGGAVVTVCAIKPADYGIATVRPNAFAAAPGGGGAAIAPLD
                     KPAGKSYRTAIEQDVEEGAGELALEEAPVVVAGGRGLGGPEPFETLLKPLAHALGGAV
                     GASRAAADAGWVPYNLQIGQTGKTVTPNLYIAVGISGAIQHKVGMRASGTIVSINKDA
                     GAAIAEFSDLLVVGDAFQIVPELTKLIEAAKSTNS"
     gene            968..2491
                     /locus_tag="VFU90_06685"
     CDS             968..2491
                     /locus_tag="VFU90_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479501.1"
                     /translation="MKRLSLTLIVALGCAFAEFAATTPTAQAGLFGGKTSTPSPSPSP
                     SALPTASPEPPSVAIPRLQAKLKANPNDQQAMSELAAEFLQINRPDLTGQLTQHLLQV
                     GDKTAQVYYYDGVAASELGNVRVAMYDLEQASNLDPTNMGVLAQLADIYLKTNRPADA
                     ERIAKRAVTFNKDQPMAIMTLGSIYASEQKFDDARTQFELAYALNKKDTTPLYQIATT
                     YAQQNNIPMALQTIDRALMVDPKSVQSLVFKADLYAKQHDDARTSAAYDDAIVAAPSD
                     DEKAQIMVRKASYFISEKKNPQGEAILQQTVTQFPKVPAGFVAYGDYYAQVHQWDKAN
                     QMLKQALTLEPDDAPALLALGEIAMQTGHAGDSVTYLKHYTQVSPDAQGFALLGQAYS
                     QTHNYAGARDSCGKSFEIQRSPETLSCVAGADFELKNYKEAAQIFDVLDKNAKGFLDQ
                     NPTLLYIAAKSYQNANECTKAVAAYKRLLPMMRKGTKEYTNVKQSASDPCHPVKKTP"
     gene            2464..3471
                     /locus_tag="VFU90_06690"
     CDS             2464..3471
                     /locus_tag="VFU90_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5479502.1"
                     /translation="MPSGEENALKFVTDPIYGDHLRGLGHPESPDRVEVVASRLRAAG
                     VLSDPLTARDANDAELLRAHAPRYLELVKRETEATAGPRYLSTGDVVVDDRSLAVARR
                     AAGGAIVAAETAAAGTGVFALVRPPGHHAEPVRGMGFCLFNNAVVAARAYQAATGGRV
                     LIADFDYHHGNGTEASAGDGLSYVSTHAAPAYPGTGYRSYRLGEDAVANVPLPASGIS
                     TEGFLAVWEALLPRVAALVRPDLLLVSAGFDYVAGDPVGDLGVDVSAAEGLAATVNRV
                     AAEHGGRVAYVLEGGYDLDALTKSIELIARAEGRGAAETSGAEAGSIPAPVAATLSQI
                     S"
     gene            3595..>4663
                     /locus_tag="VFU90_06695"
     CDS             3595..>4663
                     /locus_tag="VFU90_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5479503.1"
                     /translation="MIRTSLRHVVTALVLLVAFLSQGTWALAGTTGGLSGSVVDAETS
                     APVAGAQVTVASPSQNATGTTDASGHFTFLTLPPDTYTVTIAKNEYQTISVPGQVVFA
                     DTVQTLTVRLPKALKTIAHVSATGVGTLVKAGTTADIYSVNAGMQKAASALGGGGSLN
                     SAYSAVASVPGAYVPANQTGYFQTVTIRGGDYDQVGYEFDGVPVNRSFDNYPSSSASS
                     LGNAEVQVYTGANPANSESQGLSGYINQVIKTGTYPGYAQAELGIGTPAYYHRAMVEA
                     GGSTPDRLFSYYVGVAGYNQDFNYVDNQNGASLDNWVGAPMSLAPNLSYAPTVNYLVG
                     APAPWYYFMGPFNYALLSNISA"
BASE COUNT          905 a         1558 c         1482 g          718 t
ORIGIN      
        1 gtcacgttcg agaccgcgac gcaggcgcgc aaactcgccg acgcgctcgg cgggaaagcg
       61 cacgccgtcg tgctcggcag cggtgcggcc gcactcgccg atcagctcaa ggcgtatccg
      121 ctcgatacga ttcacgtcaa cgacgattcg gacgtcgaca aattcttgct cgatcccgtg
      181 gtcgactatc tggaggcggt cgcgcgggcc accggccccg cgctggtgct cgttccgaac
      241 acgctttccg ggcgcgacgt tgccggccgg ctggtcgctc gattgggcgc ggggctgggc
      301 gccgacgtta ccgactttac ggttgcaaac ggcagcgtcg agtgcgtctc gccgaagatg
      361 ggcggcgcgg tggtcaccgt gtgcgcgatc aaaccggccg actacggcat cgccaccgtc
      421 cggcccaacg cctttgcggc cgcacccggc ggcggcgggg cggcgatcgc tccgctcgat
      481 aagcccgccg ggaaaagcta tcgcacggcg atcgagcaag acgtcgagga aggcgcgggc
      541 gagctggcgc tcgaggaagc gccggtcgtc gtcgcaggcg ggcgagggct cggcggcccg
      601 gaaccgttcg aaacgttgtt gaagcccctc gcgcacgctc tcggcggcgc ggtcggcgcg
      661 tcgcgcgcgg ccgccgacgc cggttgggtg ccgtacaact tgcaaatcgg tcaaacggga
      721 aaaaccgtca cgccgaatct ctacatcgcg gtcggcatct cgggcgcgat tcaacacaag
      781 gtcggcatgc gcgcctccgg gacgatcgtt tcgatcaata aggatgccgg cgcggcgatc
      841 gcggagttct ccgatctctt ggtcgtcgga gacgcgttcc aaatcgtacc cgaactgacc
      901 aaactcatcg aagccgccaa gagcacgaac tcataggctc gagcgttata cgaacgtcaa
      961 aggaattatg aagagactct ccctcaccct gatcgtcgcc ctcggctgtg cttttgcgga
     1021 atttgccgca acgacgccga ccgcgcaggc cggcttgttc ggcggaaaaa cgtcgacgcc
     1081 cagtccgtcg ccgtccccgt cggcgctccc gacggccagc ccggaaccgc ccagcgtcgc
     1141 aattccgcgc ctccaggcga agcttaaagc caatcccaac gatcaacagg cgatgtcgga
     1201 actggcggcc gaatttctgc agatcaatcg ccccgatctc accgggcagc tcacgcagca
     1261 tctcttgcag gtgggcgaca aaaccgcgca ggtctactac tacgacggcg tggccgccag
     1321 cgagctgggg aacgtgcgcg tcgcgatgta cgacttagag caagcgtcga acctcgatcc
     1381 caccaacatg ggcgtcctcg cacaactggc cgacatctac ctgaagacca accgtccggc
     1441 cgacgccgaa cggatcgcga agcgagcggt caccttcaac aaggatcagc cgatggcgat
     1501 aatgacgctc ggcagcatct atgcctcgga acaaaagttc gacgacgccc gcacgcagtt
     1561 cgagctggcc tacgcgctca ataagaagga cacgacgccg ctctaccaga tcgcgacgac
     1621 gtacgcccag caaaacaaca ttccaatggc gctgcagacg atcgatcgcg ccttgatggt
     1681 agatcccaag agcgtgcagt cgctggtgtt caaggccgac ctctacgcca agcagcacga
     1741 cgacgcgcgc accagcgctg cctacgacga cgccatcgtc gccgcgccga gcgacgacga
     1801 aaaagcacag atcatggtgc gcaaagccag ctacttcatc tccgagaaga agaacccgca
     1861 aggcgaagcg atcctgcagc agacggtcac gcagttcccg aaggttcccg ccgggttcgt
     1921 cgcctacggc gactactacg cgcaagtgca tcagtgggac aaggccaacc agatgctcaa
     1981 gcaggcgctg acgctcgagc ccgacgacgc gcccgcgctg ctcgcgctcg gcgagatcgc
     2041 gatgcaaacc ggacacgccg gcgacagcgt gacgtacctg aagcactaca cgcaagtctc
     2101 gcccgacgcg caaggattcg cgctgctcgg acaggcgtac tcgcaaacgc acaactacgc
     2161 cggggcgcgc gactcgtgcg ggaagagctt cgaaatccaa cgctcgcccg aaacgctcag
     2221 ctgcgtcgcc ggtgcggact tcgaactaaa gaactacaag gaagccgcgc agattttcga
     2281 cgtgctcgac aagaacgcca aaggcttcct cgaccagaat ccgaccctgc tgtacatcgc
     2341 ggcgaaatcg taccagaacg ccaacgagtg cacgaaggcg gtggcggcct acaagcggct
     2401 gctcccgatg atgcgcaaag gtaccaagga atacaccaac gtgaaacagt cggcgtccga
     2461 tccgtgccat ccggtgaaga aaacgccctg aagttcgtca ccgatccgat ctacggcgac
     2521 cacctccgcg ggctcggcca tcccgaatcg cccgaccgcg tggaggtggt cgcttcgcgt
     2581 ttacgagcgg ccggagtgct ctcggacccg ctgacggcgc gggacgcgaa cgacgccgaa
     2641 ctgctgcggg cgcacgcgcc gcgctacctg gagctggtca aacgtgagac ggaggcgacg
     2701 gcggggccgc gctacctctc gaccggcgac gtggtcgtgg acgaccgttc gctcgcggtg
     2761 gcgcggcggg cggccggcgg ggcgatcgtg gcggccgaga cggcggcggc cggaaccggc
     2821 gttttcgccc tggtgcgtcc gccgggacat cacgccgagc cggtgcgcgg gatgggcttc
     2881 tgcctgttca acaacgcggt cgtggcggcc cgagcctacc aggcggcgac cggcgggcgg
     2941 gtgctgatcg ccgacttcga ctaccatcac ggcaacggta cggaggcgag cgccggcgac
     3001 gggctgtcgt acgtctcgac ccacgccgcg ccggcgtatc ccgggacggg gtaccggagc
     3061 tatcggctgg gcgaggatgc cgtcgcaaac gtccccttgc ctgcttcggg catttctacc
     3121 gagggattcc tcgccgtctg ggaggcgctg ctgccccggg tcgcggcctt ggtccggccc
     3181 gatctgctgc tggtgagcgc gggcttcgac tacgtggccg gtgacccggt gggggacctc
     3241 ggcgtcgacg tgagcgcggc cgaagggctg gcagccaccg tcaaccgggt cgcggcagaa
     3301 cacggcgggc gcgtcgccta cgtgctcgag ggtggttacg acctcgacgc gctcactaag
     3361 tcgatcgagc tgatcgcgcg cgccgagggc cgcggagcgg ccgaaacgag cggcgccgag
     3421 gccggatcga tccccgcgcc ggtggccgcg acgctcagcc aaatctcata agaggcccaa
     3481 ggaggcgagc ccggggctgc gaactagcgt tctcagcggg ccgatgaagg aaaatccagg
     3541 ctgggttttc ctgatttggc ttggccaaca aagcaactac ctgaggaggt attaatgatc
     3601 cggacatcgc tccggcacgt cgttaccgct ctggtacttc tcgtcgcctt ccttagccag
     3661 ggaacatggg cactggctgg aacgacgggc ggtctcagcg gcagcgtcgt tgacgccgaa
     3721 acatcggctc cggtcgccgg tgcgcaagtg acggtcgcaa gcccgtcgca aaacgccacc
     3781 ggtaccaccg atgccagcgg tcactttacg tttctgacgc ttccgccgga cacgtatacg
     3841 gtgaccatcg ccaagaacga atatcaaacg ataagcgttc cgggccaagt cgtcttcgca
     3901 gatacggtcc aaacgctgac cgtgcgcctg ccgaaggcgc tcaagaccat cgcgcacgtc
     3961 tctgccaccg gcgtcggaac gctggtgaaa gccggtacca cggccgatat ttattcggtc
     4021 aacgccggca tgcagaaagc cgcgtccgca ctgggcggcg gcggatcgct gaactcggcc
     4081 tactcggccg tcgccagcgt tccgggcgcg tacgtacccg ccaaccaaac cggctacttc
     4141 caaaccgtca cgattcgcgg cggtgactac gaccaagtcg gttacgagtt cgacggcgta
     4201 ccggtaaacc gttcgttcga caactacccg tcgagctcgg cctcgtcgct cggtaacgcc
     4261 gaagttcagg tgtacaccgg cgcgaacccg gcgaactcgg aaagccaggg tttgtcgggc
     4321 tacatcaacc aagtcatcaa gaccggtacc tatccgggct acgcgcaagc ggagctgggc
     4381 atcggcacgc ccgcctacta ccatcgcgcg atggtagaag ccggcggctc gacgcccgac
     4441 cgcctgttct cgtactacgt cggcgtggcc gggtacaacc aggacttcaa ctacgtcgac
     4501 aaccagaacg gggcgagcct cgataactgg gtcggcgcgc cgatgtcgct ggcgcccaat
     4561 ctcagctacg cgcccacggt caactacctc gtcggtgctc cggcgccgtg gtactacttc
     4621 atgggcccgt tcaactacgc gttgctgtcg aacatctcgg cac
//
LOCUS       DASUCJ010000565         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_785209, whole genome shotgun sequence.
ACCESSION   DASUCJ010000565 DASUCJ010000000
VERSION     DASUCJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785209"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..292
                     /locus_tag="VFU90_06700"
     CDS             152..292
                     /locus_tag="VFU90_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479504.1"
                     /translation="MAQQDYIPKVGDQIRYVYQGVTYIRVVVGVEDRSDRGYFRLFTQ
                     KV"
     gene            323..>1038
                     /locus_tag="VFU90_06705"
     CDS             323..>1038
                     /locus_tag="VFU90_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEU5479505.1"
                     /translation="MPPRNVPENRTIDVDVRDLHTQGRTVSGYAAVYDVESTDLGGFR
                     ERIAPGAFRTVLGADADVRCLLNHDPNIVLGRTRAGTLRLSDDARGLRFEVELPESRT
                     DIREAIARGDVDGASFRFDVARDSWLGEVRTLEEIRTLHDVSIGTYPAYPAASIELRT
                     REPAPASTPSPASGLIVRDRAPRFRRSGAERPTLAGEFRAAGFPGGHGERATIDFDRF
                     FAVGRGSEQRAYGDNPGVTYS"
BASE COUNT          160 a          365 c          322 g          191 t
ORIGIN      
        1 ggtcagccgc tgcgttcgcg tttctgaacc cgcagggtaa gacgcttccg tgggatcagc
       61 cgccgcccgt caacatcgac gttcccgcat ggtcttactg ttacggccgc tacaacctcg
      121 acgatctctg tgtcgtcgat acgggcgcct gatggctcaa caggactaca taccgaaggt
      181 gggcgatcag atccgctacg tctaccaggg cgtgacgtac attcgtgtcg tcgtcggcgt
      241 cgaggatcgt tctgaccgcg gctactttcg cctgttcacg cagaaggtct aaacgtccgc
      301 cggtgctgta ggattccgcg taatgccgcc ccgcaacgtt cccgagaatc gaacgatcga
      361 cgtcgatgtt cgtgatctcc acacgcaggg caggaccgta agcggctacg ccgcggtcta
      421 cgacgtcgaa tcgaccgatc tcggcgggtt tcgagagaga atcgcccccg gcgccttccg
      481 caccgtcctg ggtgctgacg ccgatgtgcg gtgtctactg aaccacgacc cgaacatcgt
      541 gctcggccgc acccgcgccg gcacgttgcg tctgtcggac gacgcgcgcg gcctgcgctt
      601 cgaggttgag cttcccgagt cgcgcaccga cattcgcgag gcgattgccc gcggcgacgt
      661 tgacggcgcg agcttccgtt tcgacgtggc acgcgactca tggctcggcg aggttcgcac
      721 gctcgaagaa atccggactt tgcatgacgt ctcgatcggc acgtaccccg cgtaccctgc
      781 cgcctccatc gagctacgga cccgcgagcc ggcaccggca tccactccgt cgccggcttc
      841 cgggttgatc gtccgcgacc gcgcgccgcg tttccgtcgc tcgggcgccg agcgcccgac
      901 gctcgcaggt gagttccgcg ccgccggctt cccgggcggg cacggcgagc gcgccacgat
      961 cgacttcgac cgcttcttcg ccgtcggccg tggcagcgag cagcgcgcct acggcgacaa
     1021 tccgggcgtc acctactc
//
LOCUS       DASUCJ010000566         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_464652, whole genome shotgun sequence.
ACCESSION   DASUCJ010000566 DASUCJ010000000
VERSION     DASUCJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464652"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFU90_06710"
     CDS             <1..1155
                     /locus_tag="VFU90_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5479506.1"
                     /translation="EFQFGTNWARFSSAAGGVVGFTLAMEGLFAFFAESTFLGLFLYG
                     EKKLGPRGHFVAASMVFAGSWLSGYFIIVTNAFMQHPLGYTLSPDGVFSLADFGAYVF
                     NPWAIWEYAHTMTAAVITGAIVVASIAAFWLLQGQFTRHAQAALRVAIMTGLAAAVLQ
                     LFPTGDRQGKLVAEYQPIALAAMEGKFESTARAELAIVGQPNVEERRLENPIVVPGVL
                     SFLAYGSFGATVVGLNDAPRDQWPDNVELLYFAYHIMVGLGTLLLALLGIAAILLWRG
                     TLFRTRSVLWTLMLAAPFPYIATTAGWTTAELGRQPWVVYGILRTSRASSTSVAAGDV
                     AFTTIGFMGLYLLVGMLFVVLLLRQIARGPAAHADPRSGVTPTPPVARAT"
BASE COUNT          168 a          406 c          368 g          233 t
ORIGIN      
        1 gagttccaat tcggcacgaa ctgggcgcgc ttttcgagcg ccgcgggcgg cgtcgtcgga
       61 ttcaccctcg cgatggaggg gctctttgca ttcttcgccg aatccacgtt cctagggctg
      121 ttcttgtacg gcgaaaagaa gctcggcccg cgcgggcact tcgtcgctgc ttcgatggtc
      181 ttcgccggct cgtggctgtc cggctatttc atcatcgtca cgaacgcttt catgcagcat
      241 ccgctgggct acacactctc gcccgacggc gtcttttccc tcgccgactt cggcgcgtac
      301 gtcttcaatc cctgggccat ttgggaatac gcgcacacga tgacggcggc ggtcatcacg
      361 ggcgcgatag tcgtcgcgtc gatcgccgca ttctggctgc tccaaggcca gttcacccgc
      421 cacgcgcaag ccgcgctccg cgtcgcgatc atgaccggcc ttgccgcggc cgtgctccag
      481 ctcttcccga ccggtgaccg tcagggcaag ctcgtcgcgg aataccagcc gattgcgctg
      541 gccgccatgg agggcaagtt cgagagcacg gcgagggccg aactggccat cgtcggtcag
      601 cccaacgtcg aagagcggcg cctcgagaat cccatcgtcg tgcccggtgt cctgagcttt
      661 cttgcatacg gatcgtttgg agccaccgtc gtcgggctga acgacgcgcc gcgcgaccaa
      721 tggcccgaca acgtggagct cctgtatttc gcgtaccaca tcatggtggg cctcgggacg
      781 ctgctcctcg cgctcctcgg catcgcggcg atcttgctct ggcgcggcac actgttccgg
      841 acgcgttccg tgctctggac cttgatgctc gccgccccct tcccttacat cgcgacgacc
      901 gcgggatgga ccaccgcgga gctcgggcgg cagccgtggg tcgtctatgg aatcttgcga
      961 acgagccgcg cgtcgtcgac gtccgtcgcc gccggcgacg ttgctttcac cacaatcggg
     1021 ttcatgggtc tctacctgct cgtcggaatg ctcttcgtcg tgctgctcct gcggcaaatc
     1081 gcgcgcggac cggcggcgca cgcggatccg cggagcggcg tgactccgac gccgccggta
     1141 gcccgagcca cctgaagctc aggagtgggg cgctc
//
LOCUS       DASUCJ010000567         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_724174, whole genome shotgun sequence.
ACCESSION   DASUCJ010000567 DASUCJ010000000
VERSION     DASUCJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724174"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFU90_06715"
     CDS             <1..425
                     /locus_tag="VFU90_06715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5479507.1"
                     /translation="VLPLRSPLDQYQLRSDFGPRIDPINHRASFHPGLDLAAPYKSAV
                     YSTGPGTVVFTGTREGYGRMVEIDHGHGIVTRYAHLHRILVARGQKVAAHTKVGELGS
                     TGRSTGPHVHYEVDVDGTPLDPAKFMEAGKNVVQISGK"
     gene            400..873
                     /locus_tag="VFU90_06720"
     CDS             400..873
                     /locus_tag="VFU90_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5479508.1"
                     /translation="MWFKSAANNNVGGTSQRRLAPSILSDGFRVEGDVVCSGEVHVDG
                     ALSGNLTAAKITIGEGGSVSGTVEAENAVINGSLSGRLVAVDVVLGRSSHVTADIVYC
                     SMRIEPGAVFEGYSRRVERDDVPASDTKQLPASVESLHSAADQVDEIAAHAEVGH"
     gene            complement(867..>1236)
                     /locus_tag="VFU90_06725"
     CDS             complement(867..>1236)
                     /locus_tag="VFU90_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5479509.1"
                     /translation="VVAAEPEQLDDMRRSLLAGARVANDPAARSICDALLAPMPGEIT
                     FALARRLLAGGVAVSDAEVRHAMRVAFEELKLVLEPGGAAALAAVLANKIPVRGKTVA
                     VVASGGNVDRATFAAALADQ"
BASE COUNT          220 a          417 c          400 g          199 t
ORIGIN      
        1 aggtcctgcc gctccgctcg ccgctcgatc aataccagct cagaagcgat ttcggccccc
       61 gcatcgatcc cattaatcac cgggcgtcat tccatccggg cctcgatctg gcggcaccgt
      121 acaagagcgc ggtctacagc accggtccgg gcaccgtcgt cttcacgggg actcgcgagg
      181 gctatggccg catggtcgaa atcgaccacg gtcacggaat tgtcacgaga tacgcgcatc
      241 tccatcgcat tctcgttgcc cgcggccaga aggttgccgc ccacaccaaa gtcggcgagc
      301 tcgggagtac cggacgcagc accgggccgc acgtgcatta cgaagttgac gttgacggga
      361 cgccgctcga tccggcgaag tttatggaag caggcaagaa tgtggttcaa atcagcggca
      421 aataataacg tgggcggcac ctcgcagcgc cgccttgccc cgtcgatctt gagcgacggg
      481 ttccgagtcg aaggcgacgt cgtgtgctcg ggcgaggttc acgtcgacgg cgcgctttcc
      541 ggcaatttga cggcggccaa gatcaccatc ggcgaaggcg gttccgtcag cggcaccgtc
      601 gaagccgaga acgcggtcat aaacggctcc ctgtccgggc ggctggtggc cgtcgatgtc
      661 gtgctcggcc gttcctcgca tgtgaccgcc gacatcgtct attgctcgat gcgcatcgag
      721 ccgggtgcgg tgttcgaggg ctatagccgc cgcgtcgagc gtgacgacgt tcccgcgtcg
      781 gacaccaagc agctaccggc ctcggtcgag agcttgcata gcgccgccga ccaggtcgac
      841 gaaatagcgg cgcacgccga agtcggtcac tgatcggcaa gcgcggccgc aaaggtcgcg
      901 cggtcgacat ttccgcccga cgccaccacg gcgaccgtct tgcctcggac ggggattttg
      961 ttggcaagca ccgcggcgag cgccgctgcg ccgcccggct ccagcacgag cttcaactcc
     1021 tcgaaagcga cgcgcatcgc gtgccgtacc tccgcgtcgc tgacagccac gccgcctgcg
     1081 agcagccggc gcgccaaggc gaaggtgatc tctccgggca tcggcgcgag cagagcatcg
     1141 cagatcgagc gcgccgcagg atcgttggcg acgcgcgcgc ccgcgagcag cgaacgccgc
     1201 atatcgtcga gttgctcggg ttcggccgcg acgacc
//
LOCUS       DASUCJ010000568         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_97928, whole genome shotgun sequence.
ACCESSION   DASUCJ010000568 DASUCJ010000000
VERSION     DASUCJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97928"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFU90_06730"
     CDS             <1..583
                     /locus_tag="VFU90_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479510.1"
                     /translation="DGTTFDSMYGLLGVAAYTIETNGFDFFEDCATFESDTAPRNLEA
                     LRYISRALHAPYRLPAGPDTIDVSASAAQIAAGDPLTITTHLDSSRFNNSNGTEPVHN
                     IAGASAYVDALPWDAAAVGIPLVANDGMFNSPQETAQGSIPTSDLVPGRHFVYVQAFS
                     LDVPDRLFANGFDDTTDGRRTGTPNAVFFDVTP"
     gene            635..>1001
                     /locus_tag="VFU90_06735"
     CDS             635..>1001
                     /locus_tag="VFU90_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479511.1"
                     /translation="MQKGLLGILVGLTFAGAASADEWVVEAHFADQAALQRAARHFEH
                     VIVDRQRNMLRIDTNDAGISTLESEGLSVQIDLAASARVQSLAAQVDAARAQGNGIDS
                     IPGFECYRTVEETYATLTGL"
BASE COUNT          184 a          350 c          304 g          163 t
ORIGIN      
        1 cgacggcacc accttcgact cgatgtacgg cctcctcggc gtcgccgcgt acacaatcga
       61 gacgaacggc ttcgacttct tcgaagattg cgcgacgttc gagagcgaca ctgcgccgag
      121 aaacctcgag gcgctgcgct acatctcgcg cgcgctgcac gcgccctacc gcctgccggc
      181 gggtcccgac acgatcgacg tatcggcaag tgccgcgcag atcgcggcgg gcgatcccct
      241 gacgatcacg acgcacctgg acagcagccg cttcaacaac tccaacggta ccgaacccgt
      301 ccacaacatc gccggcgcct cggcctacgt cgatgcattg ccgtgggatg ccgcggcggt
      361 cgggataccg ctcgtcgcga acgatggcat gttcaactcg ccacaagaga ccgcgcaggg
      421 atcgattccc acctcggatc tcgtgcccgg ccggcatttc gtctacgtcc aggcattcag
      481 tctcgacgtg cccgaccgcc tgttcgcgaa cgggttcgac gacacaaccg acggccgtcg
      541 cacgggaacg cccaatgccg tcttcttcga tgtcacaccg taggcgttcg cgcggcgcgc
      601 agctatgatc gcccctccaa tcggggggtc gatcatgcag aagggcttgc tgggcattct
      661 cgtcggcttg acattcgccg gcgccgcgag tgccgacgag tgggtcgtcg aagcgcattt
      721 cgccgatcag gccgcgctgc agcgcgcggc gcgtcatttc gagcacgtca tcgtcgatcg
      781 ccagcgcaac atgttgcgca tcgacaccaa tgacgccggc atcagcacgc tcgaaagcga
      841 agggctatcg gtgcagatcg acttggccgc gagcgcccgc gtgcagtccc tcgccgcaca
      901 ggtcgacgca gcccgtgcgc aagggaacgg aatcgacagc atcccgggtt tcgagtgcta
      961 ccgcacggtc gaagaaacct atgcgacgct gaccggcctg g
//
LOCUS       DASUCJ010000569         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_984134, whole genome shotgun sequence.
ACCESSION   DASUCJ010000569 DASUCJ010000000
VERSION     DASUCJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984134"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="fdh3B"
                     /locus_tag="VFU90_06740"
     CDS             complement(<1..455)
                     /gene="fdh3B"
                     /locus_tag="VFU90_06740"
                     /inference="COORDINATES: protein motif:HMM:NF038355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEU5479512.1"
                     /translation="MARMKFLCDAERCIECNGCVTACKQEHDIPWGVNRRRVVTINDG
                     VPGERSISVACMHCSDAPCMAVCPVDCFYRTDDGVVLHDKDLCIGCGYCFYACPFGAP
                     QFPQTGAFGVRGKMDKCTFCAGGPEPNNSEAEFTKYGRNRLAEGKLPACA"
     gene            complement(484..>1162)
                     /locus_tag="VFU90_06745"
     CDS             complement(484..>1162)
                     /locus_tag="VFU90_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEU5479513.1"
                     /translation="GKNWKTDLSGGIQRVCMKHGVHFWGNAKARAVVWNFPDGIPQHR
                     EPLYSPRPDLVAKYPAQDDKKAFWRLPTLFKSVQEKNKDIGRDFPLIMTSGRLVEYEG
                     GGEETRSNPWLAELQQVNFVEINPKDANDRGVRDGEWVWVKTPTGAQIKVQAMVTDRV
                     GPGTTFIPFHFSGWWQGQDMLEYYPEGAHPIVRGEAVNTATTYGYDSVTMMQETKTTL
                     CQVAKFA"
BASE COUNT          176 a          386 c          379 g          221 t
ORIGIN      
        1 gcgcacgccg ggagcttccc ctcggcgagg cggttgcggc cgtacttcgt gaattccgcc
       61 tcggagttgt tcggctccgg gccgccggcg cagaaagtgc acttgtccat cttgccgcgc
      121 acgccgaacg cgccggtctg cgggaactgc ggcgcgccga acgggcacgc gtagaagcag
      181 tagccgcagc cgatgcagag atccttgtcg tgcaggacga cgccgtcgtc ggtacggtaa
      241 aagcagtcga ccgggcagac ggccatgcag ggcgcgtcgg agcagtgcat gcacgcgacc
      301 gagatcgagc gctcgccggg aacgccgtcg ttgatcgtga cgacgcggcg gcggttcacg
      361 ccccatggaa tgtcgtgttc ctgcttgcac gcggtgacgc agccgttgca ctcgatgcag
      421 cgctcggcgt cgcagagaaa cttcattcgg gccatcgttg cctcctatcc ctgtccgtgc
      481 gcgtcaggcg aacttcgcga cctggcagag cgtcgtcttg gtttcctgca tcatcgtcac
      541 cgagtcgtaa ccgtatgtcg tcgcggtgtt gaccgcctcg ccgcgaacga tcggatgcgc
      601 gccctcgggg tagtactcga gcatgtcctg accctgccac catcccgaga aatggaacgg
      661 gatgaacgtg gttcccgggc cgacacgatc ggtgaccatc gcctgcacct tgatctgcgc
      721 gccggtgggc gttttcaccc agacccactc gccgtcgcgt actccgcgat cgttggcgtc
      781 cttcgggttg atctcgacga agttcacctg ctgcagctcg gcgagccacg gattcgagcg
      841 cgtctcctcg ccgccgccct cgtactcgac gagacggccg ctggtcatga tcagcgggaa
      901 gtcgcggccg atgtccttgt tcttctcctg cacgctcttg aacagcgtcg gcaggcgcca
      961 gaaggccttc ttgtcgtcct gcgccgggta cttggcgacg aggtcgggcc gcggcgagta
     1021 gagcggctcg cggtgctgcg gaatgccgtc ggggaagttc cacacgaccg cgcgcgcctt
     1081 ggcgttgccc cagaagtgca cgccgtgctt catgcacacg cgctggatgc cgcccgaaag
     1141 gtcggtcttc cagttcttcc ct
//
LOCUS       DASUCJ010000570         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_770404, whole genome shotgun sequence.
ACCESSION   DASUCJ010000570 DASUCJ010000000
VERSION     DASUCJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770404"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFU90_06750"
     CDS             complement(<1..719)
                     /locus_tag="VFU90_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5479514.1"
                     /translation="MTTIRDAADLSRDEHAELERLRAEVAAWRARFDADRKRDLAFTN
                     SGGDVDPLYTALDTASLDPAALGTPGTYPFTRGIHPTGYRGRLWTMRQFAGFGSARDT
                     NERYKYLLAHGQTGLSVAFDFPTLMGYDSDHPRSEGEVGKCGVAISSVADMETLFDGI
                     PLDQVSVSMTINGPAIILYCFLIAAAERQGVPSEKLRGTIQNDILKEYMAQHAWIYPV
                     EPALRLIVDGFAWSATHAPHW"
     gene            complement(754..>1545)
                     /locus_tag="VFU90_06755"
     CDS             complement(754..>1545)
                     /locus_tag="VFU90_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479515.1"
                     /translation="TGGALLGDRIRMEAVALDEGIFIRSMATRGSLGGLAASTREVAD
                     VLDGYGFDRILIETVGVGQSELDIARTADTTVVVLVPESGDSVQAMKAGLMEIADVFV
                     VNKADRPDAERLRHEIEMTLGLRLGGAMRNVPAHHGVDLKTLRNPAHAARQAAQDDAS
                     DDWTPPVLATIADKGEGIDALADALDRHFDYLSRTGTLDVRRRERLRERVVEAVERRL
                     RQRLWGNPDIAAWVDAQLPALEAGSLTPFGVADTLLARNAARLTQ"
BASE COUNT          235 a          534 c          519 g          257 t
ORIGIN      
        1 ttccagtgcg gcgcgtgcgt ggccgaccac gcgaagccgt cgacgatgag gcgcagcgcg
       61 ggttccacgg gatagatcca cgcgtgctgc gccatgtact ccttgaggat gtcgttctgg
      121 atcgtcccgc gcagtttctc ggacggtaca ccctggcgct ccgcggcggc gatgaggaag
      181 cagtagagaa tgatcgccgg accgttgatg gtcatggaga cggacacctg gtctaacgga
      241 atgccgtcga acaacgtctc catgtcggcg acgctcgaga tggcgacgcc gcacttcccc
      301 acctcgcctt ccgaccgcgg atgatcggag tcgtagccca tgagcgtcgg gaaatcgaac
      361 gcgaccgaga ggccggtctg gccgtgggcg agcagatact tgtagcgttc gttggtgtcg
      421 cgcgcgctgc cgaatccggc aaactggcgc atggtccaca gacggccgcg gtatccggtc
      481 gggtggatgc cgcgtgtgaa cggatacgtg cccggtgtgc cgagcgccgc gggatcgagg
      541 ctcgccgtgt cgagcgccgt gtagagcgga tcgacgtcgc cgccggaatt ggtgaacgcc
      601 agatcgcgct tgcggtcggc gtcaaaccgg gcgcgccacg cagcgacttc cgcgcgcagg
      661 cgttccagct cggcgtgctc gtcgcgactc aggtcggccg catctcggat ggtggtcatg
      721 cgtcgattcc tcgtctccgg cggcgtgcgc cggtcattgc gttaggcgcg cggcgttccg
      781 cgccagcagc gtgtccgcca ccccgaacgg cgtcagggat cccgcctcga gcgccggcag
      841 ctgcgcatcc acccaggcgg cgatgtccgg atttccccac agccgctgcc gcagacgtcg
      901 ttccaccgcc tccaccaccc gttcgcgcag ccgttcccgc cgccggacat ccaacgtgcc
      961 ggtccgtgaa agatagtcga agtgccggtc gagagcgtcc gccaacgcgt cgatgccttc
     1021 gcccttgtcg gcgatcgttg ctaacacggg cggcgtccag tcgtcggatg catcgtcctg
     1081 cgcggcctga cgcgcggcgt gcgccgggtt gcggagcgtc ttgagatcga cgccgtgatg
     1141 cgccggaacg tttcgcatgg caccgcccaa ccggagaccc agcgtcatct cgatctcgtg
     1201 ccgcaaccgc tcggcgtccg gccgatcggc cttgttgacc acgaacacgt cggcgatctc
     1261 catgagcccc gccttcatgg cctggacgga atcgccggac tcgggcacga gcacgacgac
     1321 ggtggtgtcc gcggtgcgcg cgatgtcgag ctcgctctgc ccgacgccga cggtctcgat
     1381 gaggatgcgg tcgaacccgt agccatcgag cacgtcggcc acctcgcgcg tactggccgc
     1441 gagcccgcct aacgatccgc gcgtcgccat cgaccggatg aagatgccct cgtccagcgc
     1501 caccgcctcc atgcggatgc ggtcgcccaa cagcgcgccg ccggt
//
LOCUS       DASUCJ010000571         1320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1289880, whole genome shotgun sequence.
ACCESSION   DASUCJ010000571 DASUCJ010000000
VERSION     DASUCJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289880"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFU90_06760"
     CDS             <1..625
                     /locus_tag="VFU90_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479516.1"
                     /translation="LDCSDHEPHGPYDQVISAIGRLPNVESLRLDAAGVELGRINEIV
                     TDDFENTSAAGVYAIGDVNGKPALTPVAIAAGRQLADRLFGGKPDAHLDYANIPSVVF
                     SHPPVGSVGLSEAEAHRQYGDAVKIHRTRFTPMLWSLAGREGKTMMKLVCAGDDERVV
                     GVHGIGVGMDEMLQGFAVAVKMGATYADFLRTVAIHPTSAEEFVTMN"
     gene            646..1038
                     /locus_tag="VFU90_06765"
     CDS             646..1038
                     /locus_tag="VFU90_06765"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HEU5479517.1"
                     /translation="MAAMFVLALHLAIIAFNVAGCVLIPIGAWRHWRWVRGFLWRSAH
                     LLCLAAVAVQALAGRACFLTIWQGDVSGTSPVQPLIAGWIDRLIYWPLPLWGFAVAYV
                     AVFAYVIVLWICVRPRMPWRRETHRRRA"
BASE COUNT          199 a          419 c          478 g          224 t
ORIGIN      
        1 gctggattgt tcggaccacg aaccacacgg cccgtacgac caggtcatca gcgcgatcgg
       61 gcggctgccg aatgtcgagt cgctgcggct cgacgccgcg ggcgtcgagc tcggcaggat
      121 caacgaaatc gtcacggacg atttcgagaa cacttccgcg gcaggcgtgt acgcgatcgg
      181 cgacgtcaac ggcaagccgg cgctgacgcc ggtggcgatc gcggcggggc ggcagctcgc
      241 ggatcgattg ttcggcggca agccggacgc gcatctcgac tacgcgaaca ttccgagcgt
      301 ggtgttctcg catccgccgg tcggcagcgt gggactgtcg gaggccgagg ctcacagaca
      361 gtacggcgac gccgtgaaaa tccacaggac acgcttcacg cccatgttgt ggtcgctggc
      421 cgggcgcgaa ggcaagacca tgatgaaact ggtgtgcgcg ggcgacgacg aacgcgtcgt
      481 cggcgtgcac ggcatcggcg tcggcatgga cgagatgctg cagggcttcg cggtcgcggt
      541 gaagatgggc gcgacctacg ccgacttcct gcgcaccgtc gcgatccatc cgacgtcggc
      601 ggaagagttc gtcacgatga actgaaggcg gcggggcggg cgcacatggc ggcgatgttc
      661 gtgctcgcgc tgcacctcgc gatcatcgcg ttcaatgtcg cgggctgcgt gctgatcccg
      721 atcggcgcgt ggcgccactg gcgctgggtg cgcgggttct tgtggcgatc ggcgcacttg
      781 ctgtgcctcg ccgccgtcgc ggtgcaggcg cttgcgggcc gcgcgtgctt cctgacgatc
      841 tggcagggcg acgtgtccgg cacgtcgccc gtgcaaccgc tgatcgccgg ctggatcgac
      901 cggctcatct actggccgct gccgctctgg gggttcgcgg tcgcgtacgt cgcggtgttc
      961 gcgtacgtga tcgtgctgtg gatctgcgtg cggccacgga tgccgtggcg ccgggagacg
     1021 catcggcgcc gtgcgtaacg cacgcgcttt tcacgcacgc gcaccgccgc ggtttccacg
     1081 gcgttcggcg cgaggccgcg aatcatgccg ccacggagaa cggcgaagcc gctgtagagt
     1141 gtatggatcg gtgttcggga aatgcgattc atggatgccc tctcggctga agtttccgaa
     1201 tccgccgcga acctcgaacg gcttgcggcg tggctggctg cgcgctccag cgtgaccgcg
     1261 atcaccggcg ccggcgtgag taccgaatcc ggcatccccg attaccgcga cggcgacggc
//
LOCUS       DASUCJ010000572         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1335832, whole genome shotgun sequence.
ACCESSION   DASUCJ010000572 DASUCJ010000000
VERSION     DASUCJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335832"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..703
                     /locus_tag="VFU90_06770"
     CDS             149..703
                     /locus_tag="VFU90_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5479518.1"
                     /translation="MVFRLLVLLASAALVPLAGCAPRGTPAPDFTLVADSGAPWTLSA
                     QGGKAVLLTFGFSHCPDTCPATLAKLAHLTKTRLGARASAVEIVMVTVDPKRDTPAVL
                     HEFVGRFGGPIVGLTGTPARLRDVERAYHVWAQPLPPKRAGGYDVVHGAEIYLIDGDG
                     RLRGVREDDDSESAIADAVRAILG"
     gene            706..1653
                     /locus_tag="VFU90_06775"
     CDS             706..1653
                     /locus_tag="VFU90_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5479519.1"
                     /translation="MLHFIFAAALGVIPVHGVVVDRVGPDAAIVRTDPAPGMLPPSIR
                     RYRVEPQAPANGTGIDALLDRTTQPWTLRETQIAGPFSPGLPEPGRVIPVELGSWLPA
                     ARLVDQAGRIVELDRAFRGKTTLLSFVFTRCPDRTLCPAISGKYAYLQSRVDPQRFAL
                     VEITLDPPYDSPEILRKYGASYGADPAVWSLLTGKGSTIARLLDVFRISSLRVSTSNF
                     IHSDKLFIVTPEGRIGYVADTGGWDPEAALAQARAIAGLASNPFERFKLSLIANVVAL
                     CGGSQFAGIVLLELALFFIVLLLVAGALWWVARVLWARP"
BASE COUNT          241 a          613 c          555 g          288 t
ORIGIN      
        1 cccgccgaaa ggagcaatcc cccgagcacg atccggacca cgaagaccgc ggcgttcatc
       61 cgggccttcc gttgcgccca gcgggcggcc gcgccctgct cgtgggatga aggcggcacg
      121 ctccgccgcc ggcgcggcgt agaatgagat ggtgttccgg cttctggttc tgctcgcgag
      181 tgcggcgctc gtgccgctcg ccggctgcgc gccgcgcggc actccggcgc ccgatttcac
      241 gctggtcgcc gattccggcg ccccgtggac gctctcggcc cagggcggca aagccgtctt
      301 gctcacgttc ggcttcagcc actgtccgga cacgtgcccg gcgacgctgg ccaagctcgc
      361 gcatctcacc aagacccggc tcggagcccg cgcgagcgcc gtcgaaatcg tcatggtgac
      421 ggtcgatccg aaacgcgata ctcccgcggt cctgcacgaa ttcgtcggac gcttcggcgg
      481 cccgatcgtc ggattgaccg gtacccccgc gcgtctccgc gacgtcgagc gcgcctatca
      541 cgtgtgggcg cagccgcttc cgcccaaacg cgccggcggt tacgacgtgg tgcacggcgc
      601 cgagatttac ttgatcgacg gcgacggccg cctccgcggc gtgcgcgaag acgacgactc
      661 cgaaagtgcg atcgccgacg cggtccgcgc gatcctggga taaccttgct gcatttcatc
      721 ttcgccgccg cactcggcgt cattccggtg cacggcgtcg tcgtcgatcg cgtcggcccc
      781 gatgcggcca tcgtgcgcac ggatccggcg cccgggatgc tcccgccgtc gatccggcgc
      841 taccgcgtcg aaccgcaagc gcccgcgaac ggcaccggca tcgacgcgtt gctcgatcgc
      901 acgacgcagc cgtggacgct gcgcgaaacg cagattgcgg ggccgttctc gcccggactt
      961 cccgagcccg ggcgggtgat tccggtcgag ctgggcagct ggctgcccgc tgcgcgactg
     1021 gtcgatcagg ccggccggat cgtcgaactc gaccgcgcgt ttcgcggaaa aaccacgctc
     1081 ctgtcgtttg tttttacgcg ctgccccgac cgcacgctct gcccggcgat cagcggaaag
     1141 tacgcctacc ttcagtcgcg cgtcgatccg cagcgcttcg cgctggtcga aatcacgctc
     1201 gaccccccgt acgattcgcc cgaaatcctg cgcaagtacg gggcgtcgta cggcgccgat
     1261 ccggcggtct ggtcgctgct caccggtaaa ggatcgacca tcgcgcgtct gctcgacgtc
     1321 ttccgcatca gctcgctgcg cgtcagtacg agcaacttca ttcatagcga caagctcttc
     1381 atcgtgacgc ccgaaggccg catcggctac gtcgccgaca ccggcggatg ggatccggag
     1441 gcggcgctcg cacaagcgcg cgcgatcgcc ggcttggcga gcaatccgtt cgaacgcttc
     1501 aagctctcgc tgatcgccaa cgttgtggcg ctctgcggcg gcagccagtt cgccgggatc
     1561 gtcttgctcg agctggcgct gttcttcatc gtgctgctgc tggttgccgg cgcgctgtgg
     1621 tgggtcgcgc gagtcctctg ggcgcgcccc tgagggcggc cgcaaaacga gcgaacgccc
     1681 gcagcgttga aggtccg
//
LOCUS       DASUCJ010000573         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1152220, whole genome shotgun sequence.
ACCESSION   DASUCJ010000573 DASUCJ010000000
VERSION     DASUCJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152220"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFU90_06780"
     CDS             complement(<1..1010)
                     /locus_tag="VFU90_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEU5479520.1"
                     /translation="MSGTDNSFFVADSPGVGTLFYGDGGTDDAEDPDIVLHEYGHAIQ
                     ESIAPGAFNGSSSSQARALGEGFGDYWSFASNYDATVPSGRDPFCIGDWDARCANDNP
                     DQLCGYPLGADCLRRVDGTKTMADFINSDVSGTEHQNGEIWSSALREIFITIGRRTTD
                     TLVLEATFGVAAGPTFAQIAERMLAADRTLNNGANISAICLAMTKRGILGSADCSPSP
                     RGEVTWFQSTSSTITITDPRPIVNLTLHTSIIGDAQVTLMGPDGTRGDPGAFRGKSAA
                     GQWTLVITSPQPVTLVSWSLAVQFQGDTPAATRPATSGKFIAAAGNAPGANGTRFITD
                     VRL"
BASE COUNT          174 a          333 c          336 g          180 t
ORIGIN      
        1 agccgcacat cggtgatgaa tcgcgttccg ttcgcgcccg gcgcgttgcc ggcggcggcg
       61 atgaacttgc ccgaagttgc cggacgcgtt gccgcaggcg tgtcgccctg gaactgcacc
      121 gcgagcgacc atgacacgag cgtcaccggc tgcggcgacg tgatcaccag cgtccactgc
      181 cccgccgcgc ttttgccgcg aaacgcgccc gggtcgccgc gcgtgccgtc aggtcccatg
      241 agcgtcacct gcgcatcgcc gatgatggat gtgtgcagtg tgaggttcac gatcggacgc
      301 ggatcggtga tggtgatcgt cgacgacgtg ctctggaacc acgtcacttc gccccgcggc
      361 gatggcgagc agtccgcgct gccgaggatg ccgcgcttgg tcatcgccag acagatcgcc
      421 gaaatgttcg cgccgttgtt cagcgtgcga tcggcggcaa gcatgcgctc ggcgatctgc
      481 gcgaacgtcg gtccggccgc aacgccgaat gtcgcttcga gcacgagcgt gtcggtcgtc
      541 cggcggccga tcgttatgaa aatctcgcga agcgcggacg accagatctc gccattctga
      601 tgttcggtgc cggagacatc gctgttgatg aagtcggcca tggtcttcgt gccgtcgacg
      661 cggcgcaggc aatccgcgcc taacggatag ccgcagagct gatcgggatt gtcgttcgcg
      721 cagcgcgcgt cccagtcgcc gatacagaac ggatcgcggc cggacggcac cgtcgcgtcg
      781 tagttcgacg cgaaggacca gtaatcgccg aaaccctcgc ctaacgcgcg cgcctgcgat
      841 gacgatgagc cgttgaacgc gcccggcgcg atcgactcct gaatggcgtg gccgtactca
      901 tgaagaacga tgtccggatc ttcggcgtca tccgttccgc catcgccgta gaacagcgtt
      961 cccactccgg gcgagtcggc gacaaagaat gaattgtccg tgccgctcac cgcatgcgga
     1021 tcg
//
LOCUS       DASUCJ010000574         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1259448, whole genome shotgun sequence.
ACCESSION   DASUCJ010000574 DASUCJ010000000
VERSION     DASUCJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259448"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFU90_06785"
     CDS             <1..739
                     /locus_tag="VFU90_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5479521.1"
                     /translation="ELLKRAIEITNGGFRRVLSFVRPDVTEYEVEAEFAHEFTRRRAK
                     FAYTPIVASGANGCVLHYIANDQPCRAGEMLLMDVAANYANFNADLTRTIPVDGRFTP
                     RQRAVYDAVLRVMRASLAGGVVGKLHRDWTREAQLVMNDELLSLGLITAEDVQKQTPD
                     EPACRKYFMHGLGHPLGLDVHDVGLMHEPFAPGWVLTVEPGTYIPDEGFAVRLATNVL
                     VTESGPIDLMAAIPLEAEEIEALMQRA"
     gene            complement(794..>1117)
                     /locus_tag="VFU90_06790"
     CDS             complement(794..>1117)
                     /locus_tag="VFU90_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479522.1"
                     /translation="VVTFQGKPLTNAIVSFMPAGGRPASGVTNERGEFTLSTFAPSDG
                     ALPGKHKVIVSEPTPEPKEGDYSVPEPTPSRFPEKYANPRLSPLEFEVKPGGENKFTI
                     ELKDG"
BASE COUNT          196 a          349 c          371 g          201 t
ORIGIN      
        1 cgaattgctc aagcgggcga tcgagatcac caacggcggc tttcgccgcg tgctgtcgtt
       61 cgtgcggccc gacgtgaccg agtacgaagt cgaggccgaa ttcgcccacg aattcacgcg
      121 gcgccgggcg aaattcgcct acacgccgat cgtggcctcg ggcgccaacg gctgcgtgct
      181 gcactacatc gccaacgatc agccctgccg ggccggcgaa atgctgctga tggacgtggc
      241 ggccaactat gccaacttca acgccgatct gacgcgcacg attccggtcg acggccgctt
      301 cacgccgcgg cagcgggccg tgtacgacgc cgtgctgcgc gtgatgcggg cttcgctggc
      361 cggcggcgtg gtcggcaagc tgcaccgcga ttggacccgc gaagcgcagc tggtgatgaa
      421 cgacgaactc ctatcgcttg ggctgattac cgccgaagac gttcaaaagc aaacgcccga
      481 cgaaccggcc tgccgcaagt atttcatgca cgggctgggg catccgctgg ggctcgacgt
      541 tcacgacgtc gggctgatgc acgagccgtt cgcgccgggc tgggtgctga cggtcgagcc
      601 gggcacctac atccccgacg aaggcttcgc cgtgcggctg gcgaccaacg tactcgtgac
      661 cgagtcgggg ccgatcgact tgatggcggc gatccccctc gaggccgaag agatcgaagc
      721 gttgatgcag cgtgcgtagg ttacgcttca gcgtaacctg gccggacgtt aggctaaagc
      781 gtgacctagt tgattagccg tccttgagtt cgatcgtaaa cttgttctcg ccgcccggtt
      841 tgacctcgaa ctcgagcgga gatagccgcg gattggcgta tttctctgga aaccgcgaag
      901 gcgtcggctc ggggacggaa taatctcctt ccttcggttc gggcgtcggc tccgagacga
      961 tgaccttgtg cttgcccggc aaagcgccgt cgctgggcgc gaacgtcgaa agcgtgaact
     1021 cgccccgctc gttggtgacg ccagaggccg gccttccgcc cgcgggcatg aaggaaacga
     1081 tggcgttggt tagcggcttg ccctggaagg tcacgac
//
LOCUS       DASUCJ010000575         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_785394, whole genome shotgun sequence.
ACCESSION   DASUCJ010000575 DASUCJ010000000
VERSION     DASUCJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785394"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VFU90_06795"
     CDS             complement(<1..1608)
                     /locus_tag="VFU90_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5479523.1"
                     /translation="MLRRAIPLLLLAAVTTSHAAIAGAGEATPAATEGVAHAHAYRVV
                     VDAPGNLRAVLATSVDLVRWETYAEMTAELFAELVRVAPDEAKEAAATEGYFSAKATV
                     SVDRTSEPVGVTLRVEAGPPTLVRNVEIVVTGPAASNADARSTIDALRRDWSLPRDSV
                     FTQATWQSAKARAVAMITAGPWAAAKITASEARVDPATDRADLSVTIDSGPPFRFGAL
                     DIGGLERYPPEMVRHYASFAPGDPWSRGSLDQFVRRLNGTGYFASVQATLDNDPAHAD
                     AAPVRVAVIEAPPRRVEAGVGFSTDTAFRGNLSYRDVDFASRAIQMHIDARIESKLQS
                     GAIRFVTPPAANGWSTSTFANVERTDIAGLVTQTGSAGLRMNSLDERDQWQYGAAMYE
                     DEQHPADAEAVSSHALYIDAERIWRRVDDLTSPTRGYSVLLGAGAGVPGVSTRTFERV
                     VAHAAAWWPIDRNWSAYFRAEGGAVLAASREGIPSTLLFRTGGDTTVRGYAFESLGVR
                     EGNAVVPGRYYGAASVELTRWINSALGI"
     gene            complement(1559..2056)
                     /locus_tag="VFU90_06800"
     CDS             complement(1559..2056)
                     /locus_tag="VFU90_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479524.1"
                     /translation="MTNSIEIGNLGARDCANAGPGLVIAEVSMQRATGIMLVTIAAAM
                     LLGGCALRGTRDDYMYSRSSDGLWDADYGLIDWNFPTSQNQPPLEGPKRLRPERTAIG
                     VTWATTDSWPYYHRPAHAADPNAGTVAKADEPAAKPASTSGGAAASKDAAAGNPAPAA
                     GGGNH"
     gene            2396..2941
                     /gene="mraZ"
                     /locus_tag="VFU90_06805"
     CDS             2396..2941
                     /gene="mraZ"
                     /locus_tag="VFU90_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU5479525.1"
                     /translation="MKWEDGVAAGPAANQGGNERATGASPTDGSADGIGFRGVTELSL
                     DAKGRLAVPARHREGLVNGSRGELVVTADPGGCLLIFPRLAWEPLQQQLMSFSSFNET
                     VRRLQRLLVGHADDVEIDAAGRILVPPALRRYAALDKHVVLVGQGRKLELWDAVRWQA
                     QTAQAIALPGELPTDLAGFSL"
     gene            2957..>3109
                     /gene="mraW"
                     /locus_tag="VFU90_06810"
     CDS             2957..>3109
                     /gene="mraW"
                     /locus_tag="VFU90_06810"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEU5479526.1"
                     /translation="MPGDHHVPVLLAESVAALVTRDDGTYVDATFGRGGHSRAILARL
                     AASGPLV"
BASE COUNT          478 a         1032 c         1065 g          534 t
ORIGIN      
        1 gatgcccagc gccgaattga tccagcgcgt cagctcgacg ctcgcggcgc cgtagtagcg
       61 gcccggaacg acggcgttgc cttcgcgcac accgaggctc tcgaacgcat agccgcgcac
      121 cgtggtgtca ccgccggtac gaaacaagag cgtcgatgga atgccttcgc ggctcgcggc
      181 gagcaccgct ccgccttcgg cacggaagta ggctgaccag ttgcggtcga tcggccacca
      241 ggcggcggcg tgcgcgacga cgcgctcgaa cgtgcgcgtc gatacgccgg gaacgccggc
      301 cccggctccg agcagcacgc tgtagccgcg cgtcggcgac gtgaggtcgt cgacacgtcg
      361 ccagatgcgc tcggcgtcga tgtagagcgc gtgcgacgac accgcctcgg catcggctgg
      421 gtgctgctcg tcctcgtaca tcgccgctcc gtactgccac tggtcgcgct cgtcgagcga
      481 attcatccgc aggccggcgg aaccggtttg cgtgacgagg ccggcgatgt cggtgcgctc
      541 gacgttcgcg aacgtcgacg tcgaccagcc gttggcagcc ggcggcgtca cgaagcggat
      601 cgcgccgctc tgcagcttcg attcgatcct tgcgtcgatg tgcatctgga tcgcccgcga
      661 cgcgaaatcg acgtcgcgat aggaaaggtt gccgcggaat gccgtatcag tggagaagcc
      721 gacgccggct tcgacgcgcc gcggcggcgc ctcgatcacc gcaactcgca ccggcgccgc
      781 gtcggcgtgc gcggggtcgt tgtcgagcgt cgcctgtacg ctcgcgaaat agccggtgcc
      841 gttgaggcgg cgcacgaact ggtcgagcga accgcgcgac cacgggtcgc cgggagcgaa
      901 gctcgcgtag tggcggacca tctcgggcgg atagcgctcg agaccgccga tgtcgagcgc
      961 gccgaagcga aacggcggcc cgctgtcgat cgtcaccgag agatcggccc ggtcggtcgc
     1021 cggatcgacg cgcgcttcgc tcgcggtgat cttcgcagcc gcccagggcc cggcagtgat
     1081 catcgcgacg gcgcgcgcct tcgcgctttg ccatgtcgcc tgcgtgaaga cactgtcgcg
     1141 cggcagcgac cagtcgcggc gcagcgcatc gatcgtcgag cgggcatccg cgttcgacgc
     1201 ggcgggtccg gtcacgacga tctcgacgtt gcgcacgagc gttggtggtc ccgcttcgac
     1261 gcgcagcgtg acgccgaccg gctcgctcgt gcgatcgacc gaaacggtgg ccttcgccga
     1321 gaagtagcct tcggtggccg cggcttcctt cgcttcgtcg ggcgccacgc gcacgagctc
     1381 ggcgaagagc tccgccgtca tctcggcata cgtctcccag cgcacgaggt cgacgctcgt
     1441 cgcgagcacg gcacgcagat tcccgggtgc gtcgacgacg acgcgatagg cgtgcgcgtg
     1501 tgcaacgcct tccgtcgcgg cgggcgtggc ttcgccggcg cccgcgatgg ctgcgtggct
     1561 agtggttacc gccgccagca gcaggagcgg gattgcccgc cgcagcatcc ttcgaggccg
     1621 cggcgccgcc cgatgtgctg gcaggtttcg cagcgggctc atcggccttc gcgaccgtgc
     1681 ccgcgttcgg atcggcagcg tgcgccggcc gatgatagta gggccacgag tcggtggttg
     1741 cccacgtcac gccgatcgcc gtgcgttcgg ggcgcagccg cttcgggcct tcgagcggag
     1801 gctgattctg cgaagtcggg aaattccaat cgatcaatcc gtagtcggcg tcccacagtc
     1861 cgtcgctcga ccgcgagtac atgtaatcgt cgcgtgtgcc acgcagtgcg cagccgccga
     1921 gcagcatcgc tgccgcgatc gtaaccaaca tgatgccagt ggcgcgttgc atcgaaacct
     1981 ccgcaatcac gagtccggga cctgcgttcg cgcaatcgcg cgcgccgagg ttcccgatct
     2041 cgatcgaatt cgtcatcgat gatcggtcga tcgagtgtac cggcaggtcg agccggcgac
     2101 caagcatgtg ccgattcatg ccaacgtcat cgggtgcgca tttgcaacac cccggagtgg
     2161 gcgtggaacc cgtccgaagc atcgctgttt tgaccggtat cgaacgaacg taccggtttt
     2221 cggccgttcg tccggcaatg gtgggagatt accttcataa attgcgctaa gtacatcatt
     2281 acatggcgct tttcgattgt tgcattcgta cgataagtcc ttgttattca atcggaaaaa
     2341 cgttgacctc gcgttcggac agcagatata gtgccgccag gtgggaaaaa gtgggttgaa
     2401 gtgggaggat ggggtggcgg ccggtccggc agcaaatcag ggtgggaacg agcgcgcgac
     2461 cggtgccagc ccgacggacg gcagcgccga cggtatcggc tttcgcggcg ttaccgagct
     2521 ctcgctggac gccaaaggcc gtctcgccgt tcccgcgcgt catcgcgagg gtctcgtcaa
     2581 cggcagtcgc ggcgagctcg tcgtcaccgc ggacccgggc ggctgcctct tgatcttccc
     2641 gcgcctcgcg tgggaaccgc tgcagcagca gttgatgtcg ttctcgtcgt tcaacgaaac
     2701 ggtgcgccgg ctgcagcgtc tgctcgtcgg ccacgccgac gacgtcgaga tcgatgcggc
     2761 ggggcgcatc ctcgttccgc cggcgctgcg tcgttacgcc gctctcgaca agcacgtcgt
     2821 gctggtcggg cagggcagga agctcgaact gtgggacgcg gtgcgctggc aggcgcagac
     2881 cgcgcaggcg atcgcgcttc ccggcgagct tcccaccgac ctcgcgggct tcagtctctg
     2941 acgctgcgat ggctcgatgc ccggcgacca tcatgtaccg gtgcttctcg ccgagagcgt
     3001 tgcggcgctc gtcacgcgcg acgacggcac ctatgtcgat gcgacgttcg gccgtggcgg
     3061 gcactcacgc gcgatcctcg cgcggctcgc agcttccggc cccctggtt
//
LOCUS       DASUCJ010000576         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_449425, whole genome shotgun sequence.
ACCESSION   DASUCJ010000576 DASUCJ010000000
VERSION     DASUCJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449425"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFU90_06815"
     CDS             complement(<1..316)
                     /locus_tag="VFU90_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479527.1"
                     /translation="MLRNLLAATALLAAATLAACGGGGGGTGTGGGLYGGVPPTPPPQ
                     QSNMPQRQTVGGKAAFVDPGNDFTLYFLDVDTPAGGACTGACLSEWFPLKPSSGATGQ
                     GNF"
     gene            609..1052
                     /locus_tag="VFU90_06820"
     CDS             609..1052
                     /locus_tag="VFU90_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479528.1"
                     /translation="MDMNPADRCPPACVLALALLFAAPGCSSEASAAMPPAAGFAPSD
                     SLRGYAVRQSPAQRCRVFAAQFDVFAGCERADGTLRSFPSATIGWRVRYFVRYQRMPC
                     NEAPTEDESYVFHMVVNDLDGDPDLNGAQRTAIRNAMFEGTPRCK"
     gene            complement(1070..>1449)
                     /locus_tag="VFU90_06825"
     CDS             complement(1070..>1449)
                     /locus_tag="VFU90_06825"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5479529.1"
                     /translation="IPVVGHIGVTPQTAGLGPGFKMRTHRDRLIDDARAIEAAGAYAI
                     VLEVVDHEISREITEMLSIPTIGIGSGPHCDSQVLVLHDILGMYSHSPSFAKRYAEIG
                     RAATEALAQYAREVRDGSFPTPS"
BASE COUNT          230 a          455 c          499 g          265 t
ORIGIN      
        1 tgaaatttcc ctgcccggtc gcaccgctgc tcggcttgag cggaaaccat tcgctcaagc
       61 aggcgccggt gcacgcaccg ccggccggcg tgtcgacgtc gaggaagtag agcgtgaagt
      121 cgttgcccgg atcgacgaac gccgcttttc cgccaacggt ctggcgttgg ggcatattcg
      181 actgttgcgg cgggggcgtt ggcggaactc cgccgtagag tccgccgccg gtcccggtgc
      241 cgccgcctcc gccgccgcag gcagcgagcg tcgcggcggc gagtaacgcc gtcgcagcga
      301 gtagatttcg taacatggcc gtgccgttct ccttgtaagc gatcgagtga ttcgaatcgc
      361 acgttaccga ggaggcgtga gaagtgtatg aagcgcgccg ggttttttgc gcgcttttag
      421 actcatttca tcgttgtgaa gaaagtgtta cgaccggatc gggctgcatc gcgacgccgc
      481 cgcaggtaac gttcaaaacc cggcaggcgc cttatcggcg cgaacgggcg ctcgtgcggc
      541 tggtttgtgc tgatgtgtat tatccggcgg cgagcggcga ggcactatcc agggtaccag
      601 ggaggactat ggatatgaac cccgccgatc gttgcccgcc agcctgcgtt ctcgccctcg
      661 cgctgctctt cgccgcgccg ggctgcagct cggaagcttc ggccgcgatg ccgccggcag
      721 ccggcttcgc gccgtccgat tcactccgcg gttacgcggt acggcagtcc cccgcgcaac
      781 gctgccgcgt ttttgccgcg cagttcgacg tattcgccgg gtgcgagcgc gccgacggga
      841 cactgcggtc gttcccgagc gcgacgatcg gctggcgcgt gcggtacttc gtgcggtacc
      901 agcggatgcc gtgcaacgaa gcgccgaccg aagacgagtc gtacgtcttt cacatggtcg
      961 tcaacgacct ggacggcgat cccgatctca acggcgcgca gcgcaccgcc attcgcaacg
     1021 cgatgttcga gggaacgccg cgctgcaaat gagcttcgag ttccccgctt taagaaggcg
     1081 tagggaagct gccgtcgcgg acttcgcggg catactgcgc gagcgcctcg gtcgccgcac
     1141 gcccgatttc cgcgtagcgt ttggcaaacg agggcgaatg cgagtacatg cccaggatgt
     1201 cgtgcagcac cagcacctgc gagtcgcagt gcgggcccga gccgatgccg atcgtgggga
     1261 tggagagcat ctcggtgatc tcgcgggaga tctcgtgatc gaccacttcg agcacgatcg
     1321 cgtacgcgcc ggcggcttcg atggcgcggg cgtcgtcgat cagccggtcg cgatgggtgc
     1381 gcatcttgaa gccggggccg aggccggcgg tttgcggggt gacgccgatg tgtccgacca
     1441 ccgggatgc
//
LOCUS       DASUCJ010000577         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_739845, whole genome shotgun sequence.
ACCESSION   DASUCJ010000577 DASUCJ010000000
VERSION     DASUCJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739845"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFU90_06830"
     CDS             complement(<1..597)
                     /locus_tag="VFU90_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5479530.1"
                     /translation="MAGLVPAIHVFTVRRKKTWMPGTRPGMTEFMTQPSASTAPIVSL
                     RNVGKTFDNGTAALDGLTLDVGDGEFVSLLGPSGCGKSTALRIIAGLSAPSAGTVEWP
                     NGKGRIGFVFQEPTLMPWADVASNVRLPLTLAHADETVARDTALQVLSHVGLGDFARS
                     YPRELSGGMKMRASIARALVTETQVLLMDEPFAALDEIT"
     gene            complement(604..>1101)
                     /locus_tag="VFU90_06835"
     CDS             complement(604..>1101)
                     /locus_tag="VFU90_06835"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479531.1"
                     /translation="ADKNAAMEGYVTSEPYAIEKRAHFKPVVFLLADYGFNSYSTLIE
                     TRRDLAAKRPDLVQRFVDASAIGWYHYLYGDARPGNALIKRQNPEMTDGQLAYSIAQM
                     KHDGIVDSGDTLKLGIGAMTDARWRSFYDKMVHAGVVKPGLDYKRGYTLQFVDKGVGV
                     DLRPK"
BASE COUNT          187 a          357 c          359 g          198 t
ORIGIN      
        1 cgtgatctcg tcgagcgcgg caaacggttc gtccatcagc aggacttgcg tctcggtcac
       61 cagcgcccgc gcgatcgagg cgcgcatctt catgccgccc gacagttcgc gcggatagct
      121 tcgcgcaaag tcgccgaggc cgacatgcga cagaacctgc agcgcggtgt ctcgcgcgac
      181 cgtctcgtcg gcatgcgcca gtgtcagcgg cagccgcacg ttcgacgcca cgtcggccca
      241 cggcatcagc gtcggttcct ggaacacgaa gccgatacgg cctttgccgt tcggccattc
      301 cacggtgccg gcgctcggcg cgctgagacc ggcaatgatg cgcagcgccg tcgacttgcc
      361 gcagcccgac ggcccgagca gcgagacgaa ttcgccgtcg ccgacgtcga gcgtcaggcc
      421 gtcgagcgcg gccgtgccgt tgtcgaacgt cttgccgacg ttgcgtagcg agacgatggg
      481 agcggtggat gcggaaggct gagtcatgaa ctccgtcatg cccggccttg tgccgggcat
      541 ccacgtcttc ttgcgtcgta cagtaaagac gtggatggcc gggacaagcc cggccatgac
      601 ggcttacttg ggccgcaggt caacgccgac gcccttgtcg acgaattgca acgtgtagcc
      661 gcgtttgtag tcgaggccgg gcttgaccac gccggcatgc accatcttgt cgtagaagct
      721 ccgccagcgc gcgtccgtca tggcgccgat gccgagcttg agcgtatcgc cggagtcgac
      781 gatgccgtcg tgcttcatct gcgcgatgga gtaagcgagc tggccatcgg tcatctccgg
      841 gttctgccgc ttgatgagcg cgttgccggg gcgcgcatcg ccgtaaagat agtggtacca
      901 gccgatggcc gaagcatcga caaaacgctg caccaggtcg ggccgcttcg ccgcgagatc
      961 gcgccgcgtc tcgatcagcg tcgagtagct gttgaagcca taatctgcga gcaggaagac
     1021 gacgggcttg aaatgcgccc gcttctcgat cgcatagggc tccgacgtga cgtagccttc
     1081 catcgccgca ttcttgtcag c
//
LOCUS       DASUCJ010000578         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1152274, whole genome shotgun sequence.
ACCESSION   DASUCJ010000578 DASUCJ010000000
VERSION     DASUCJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152274"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFU90_06840"
     CDS             <1..811
                     /locus_tag="VFU90_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479532.1"
                     /translation="APSATPVPLPSPDRLLFLVRRQFRSHRPPPPYVTYTLVRKQTLD
                     DGYPDLVNSYTYHIWCRTLDRAALGRRVYRGSARGTLEFLRPSFNEPWDPGPPTADLF
                     EPAPAHPHSNPRDFVPTPEPTGSVPPVIASVTVMGEFDYRVTGVTHEGNLLHVTLVPR
                     RDPDRNRLRDLYVDAKTLELQEVIATDKLYEEGSRHVYPMMFTVTLGWQHGMPVVTHI
                     HGTPTLESDSEYLGKDATVDYDFTDLAFPSSLPDWYFDPRSYAAHVDDAPG"
     gene            complement(808..1587)
                     /gene="msrA"
                     /locus_tag="VFU90_06845"
     CDS             complement(808..1587)
                     /gene="msrA"
                     /locus_tag="VFU90_06845"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5479533.1"
                     /translation="MRFSAVAPLLLAPLVTAPADASARTLQMIDSLEVVDVAGGASSN
                     CARHALHSRMKAFLAALLSAALLAAAPAPPASQTQRVVLAGGCFWGMQLVFGSLKGVR
                     SAVAGYAGGAAATAHYEVVSTGATGHAESVEITYDPAVISFKQLLDVYFLVAHDPTEL
                     NRQGPDDGTQYRSEIFYTTAGQQHEAQALIAQLTRSHAYSAPIVTQLAPLKGFYPAEG
                     YHQNFAVHNPDNPYIVANDLPKLRTLRAKYPQLVKPDAPFG"
     gene            1663..>2605
                     /locus_tag="VFU90_06850"
     CDS             1663..>2605
                     /locus_tag="VFU90_06850"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEU5479534.1"
                     /translation="MRAQPLISPARTVAFATVAYALIFFVLGADRYATFHSGADLGLF
                     VQTIASAFAGFHNTVEGTSHFAYHFSPILYLCAPLLWLTRSPLALVFIQAAATAAIAP
                     AMYVIAKRRTREGWAAALACIALLYPPLQGVTFTDFHEVAFVPAAVAWLLWAIDARRF
                     GIAALLLAAVLAIKEDQAPAMAFAGLAGCVYFARRGERAGVLFSASAVAASAIVFWAY
                     FALVRPLAGAPGQWIPIHFYSWNGGGPRSPLQTQILGRVTYLLEAFVPLAFVPLRSRV
                     VLLALPGFAEVLASREPLTYTMGQHYAAVWIPYVLVAF"
BASE COUNT          406 a          849 c          876 g          474 t
ORIGIN      
        1 ggcaccgtcg gcgactccgg ttccgctgcc gtctcccgat cggctgctgt ttctggtccg
       61 gcggcagttt cgatcgcacc ggccgccgcc gccgtacgta acgtacacgc tggtgcgcaa
      121 acaaacgctt gacgacggtt acccggatct cgtcaacagc tatacgtatc acatctggtg
      181 ccgcaccctc gaccgcgcgg ccctggggcg gcgcgtgtat cgcggttccg cgcgcggcac
      241 gctcgagttt ctgcggccgt cgttcaacga gccgtgggat cccggccccc cgacggccga
      301 cctgttcgag ccggcaccgg cgcacccgca cagcaatccg cgcgatttcg tccccacgcc
      361 ggaaccgacc gggagcgtgc cgccggtgat cgcgagcgtg accgttatgg gagagttcga
      421 ctatcgcgtg accggcgtga cgcacgaggg caacctgctc cacgtgacgc tggtgccgcg
      481 ccgcgacccc gaccgcaacc gtttacgcga cctctacgtc gacgcgaaaa cgctcgaact
      541 gcaggaagtg atagccaccg acaaactcta cgaggagggg agccggcacg tctacccgat
      601 gatgtttacg gtaacgctcg gctggcagca cggcatgccg gtggtcacgc acattcacgg
      661 aacgccgacg ctcgagagcg actccgagta cttagggaag gacgcgaccg tcgactacga
      721 tttcaccgac ctcgccttcc cgtcgtcctt gccggactgg tacttcgacc cgcgttcgta
      781 cgccgcacac gtcgacgacg cgccgggcta gccgaacggg gcgtcgggct tgacgagctg
      841 cggatacttg gcgcgcagcg tgcgaagttt agggagatcg ttggccacga tgtacgggtt
      901 gtcgggattg tgcaccgcga agttctgatg gtaaccctcg gcgggataga agccctttag
      961 cggggcgagc tgcgtaacga tcggcgcgga ataggcgtgg cttcgcgtca gctgcgcgat
     1021 caaagcttgg gcttcgtgct gctgaccggc ggtggtatag aaaatctcgg accggtattg
     1081 cgtgccgtcg tcgggaccct ggcgattgag ctcggtcggg tcgtgcgcga cgaggaaata
     1141 cacgtcgagc agctgcttga aagagatcac cgccgggtcg tacgtgattt cgaccgattc
     1201 ggcgtgccct gtagcgcccg tgctgaccac ttcatagtga gcggtcgccg cggcgccgcc
     1261 ggcgtagccg gcgacggcgg accggacgcc tttgagcgat ccgaaaacga gctgcatccc
     1321 ccagaaacat ccacccgcca gcaccactcg ctgggtttgc gaagccggcg gagcgggagc
     1381 ggcggccagt aaggcggcgg acaagagtgc ggcgagaaag gctttcatac gactatgtaa
     1441 cgcatggcgg gcgcaattgg atgacgcgcc cccggcgacg tcgacgacct ccagagaatc
     1501 gatcatctgc agggtgcgcg ccgatgcatc ggccggcgcg gtaaccaacg gcgcgagcag
     1561 cagcggcgct acggcagaaa atcgcatggg acgtcgttct caagcaggcc cgggctgcct
     1621 tccgagagcg aagggcgcca cgcgtgtcga acgagtaagc gcatgcgcgc gcaaccgctg
     1681 atatcgccgg cgcgaacggt cgcgttcgcg accgttgcct acgcgctgat ctttttcgtg
     1741 ctgggtgcgg accgttacgc gacgttccat tccggcgcgg acctcgggct attcgtccag
     1801 acgattgcca gcgcgttcgc gggctttcac aataccgtcg aaggcacgtc gcacttcgcg
     1861 taccactttt cgccgatctt gtacctctgc gcgccgttgc tgtggctgac gcgctcgccg
     1921 ttggcgctgg tcttcattca agccgccgcg accgccgcga tcgcgcccgc catgtacgtc
     1981 atcgccaagc gccgaacccg cgaaggctgg gccgcggcgc tggcctgcat cgcgctgctc
     2041 tacccgccgc tgcagggcgt gacgttcacg gactttcacg aagtcgcgtt cgtcccggcg
     2101 gcggttgcgt ggctgctgtg ggcgatcgac gcgaggcgtt ttgggattgc ggcgctgctg
     2161 ctcgccgccg tgctggcgat caaggaggat caagcgccgg cgatggcgtt cgcgggtctt
     2221 gccggctgcg tgtatttcgc gcgccgcggg gaacgcgccg gcgtgctctt ttcggcgagc
     2281 gcggtggccg cgtccgcaat cgtgttctgg gcgtacttcg cgctcgtccg cccgttagcc
     2341 ggcgcgcccg ggcagtggat cccgatccat ttctattcgt ggaacggcgg cgggcctcgg
     2401 agtccgctgc aaacgcaaat cctcggccgc gtcacctacc tgctcgaggc gttcgttccg
     2461 ctggcgttcg tgccgttgcg ctcgcgcgtc gtgctgctcg cgctgccggg gttcgccgag
     2521 gtgctggcgt cgcgcgagcc gttgacctat acgatggggc agcattacgc ggccgtctgg
     2581 attccatacg tgctggtggc gttcg
//
LOCUS       DASUCJ010000579         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_984251, whole genome shotgun sequence.
ACCESSION   DASUCJ010000579 DASUCJ010000000
VERSION     DASUCJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984251"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="rsfS"
                     /locus_tag="VFU90_06855"
     CDS             <1..240
                     /gene="rsfS"
                     /locus_tag="VFU90_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5479535.1"
                     /translation="FAIVTGRSKIQTRAIADAIVAAAQQERRAVSRVEGHAEGGWILI
                     DLGNLIVHVFTPEQRTFYNVERLWSEATARQAQGS"
     gene            237..383
                     /locus_tag="VFU90_06860"
     CDS             237..383
                     /locus_tag="VFU90_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479536.1"
                     /translation="MKAKRPQHETRAARGRRDFYKRVGVWIFILFFALSVAGGLIVIS
                     VVSR"
     gene            425..1747
                     /locus_tag="VFU90_06865"
     CDS             425..1747
                     /locus_tag="VFU90_06865"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5479537.1"
                     /translation="MQTLDAAAQHARYVLTPWVAQGGRRPPALVRGEGSYLYDDAGKR
                     YLDFSSGLVAVNLGHGHPAIARAIAEQAERLAYASPGFVLDKRAELAKAIVEIGPWEE
                     GGRIFFTTGGGEANEDAVKFARALTGRHKVLTAYRSFHGSAPGAGTLTGENRRWPNEP
                     GIPGVVHFFAPFPYRSPFFTRDPAEEVERAVAHLEEIVRYEGGDRIAAILIEPVVGSN
                     GVIVYPDGYLARLRELCDRHGILLIFDEVMTGFGRTGAAFAAQRFGVVPDLFTFAKGV
                     TSAYVPLGGVALRESLAAHFDTRPLPSGHTFSGHPVAMAAGLATVTAYREERTFDRAR
                     ALEPELRRRFEELQRRHPGVVGEVRGVGAFFGLELVSDPQTRRPLVDWQGTASLQTFF
                     DDLLQRGLYAFGRYNVAILAPPLNANAGELDEAAAIFDAALGKLGGTR"
     gene            1744..3141
                     /locus_tag="VFU90_06870"
     CDS             1744..3141
                     /locus_tag="VFU90_06870"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5479538.1"
                     /translation="MTTDLSVRNPATREIIGRVPVAGSRDVDAAVAAALGAFDSWSKV
                     PVVERARLMFRFADALERRRNDLALSVSRENGKTLADARAEVRRGIEAVEFACGMPSL
                     LMGDALPDVARGIDSVSVRYPLGVCAGITPFNFPAMIPLWMLPIAVAAGNAFVLKPSP
                     QTPLTAELLAETARECRFPDGLFSVVHGGAETVDALIDHPGIAAVSFVGSSKVARLVQ
                     ERAVRAHKRVQALGGAKNYLVVMPDGVTRATVDAVISSAFGGAGQRCLAGSVAVAVGE
                     AGDRFVPMLVEAARALRLGPGTDEDVDMGPVVSDEAFARVEGYVARARAAGERVLLEP
                     AASPATGSFLSPAIFDSVSPSSELVREEIFGPVLAVVRAATLDEAIALANASRYGNAS
                     SIFTASGAAARTFASRIAVGMVGINVGVAAPMAFFPFGGVKDSIFGDLRCHGKDAVAF
                     YTQQRVVMSRWAEPS"
     gene            3138..>4138
                     /locus_tag="VFU90_06875"
     CDS             3138..>4138
                     /locus_tag="VFU90_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5479539.1"
                     /translation="MNEPPALRSGSLTFLEVLATSVALIGPSMTPILIAPYMFANAGN
                     GTWLAYAFGGTMLVFVALNINQFARRSSEAGSMYGYVAANLGPMLGAIAGWSLLWAYG
                     FVASAVLGAMALFVELLLGYGNLHAPAVAIVCAIAVVAWQAAYRGVQVSAIVMLVLEA
                     ISVTVICVLIGIVLVKHGPSLDVSQVKIAGGFPGGIGLAIMFAVFSFVGFESATAFGA
                     EARNPLVTIPRAVIGSVVFVSIFFIVATYAEVLGLRGAPIPLDQQTFPLGTLANVFDV
                     GYLRIPIAIGALASAFSVCLACITTAGRVAYAMAVSGLLPPSFARIEPRHDTPNVAVT
                     "
BASE COUNT          608 a         1392 c         1390 g          748 t
ORIGIN      
        1 tttgcgatcg tcacgggacg gtcgaaaatt cagacgcgcg cgattgccga cgcgatcgtc
       61 gcggcggcgc agcaggagcg gcgggcggtc tcgagggtcg aagggcatgc cgagggcggg
      121 tggatattga tcgacctcgg caacttgatc gtccacgttt tcacgcccga gcagcgcacg
      181 ttctacaacg tcgagcgcct ctggtcggag gctaccgcgc gccaggccca gggctcgtga
      241 aggccaaacg gccgcagcac gaaacgcgcg ccgcccgcgg acggcgcgat ttctacaagc
      301 gcgtcggggt ctggattttc atccttttct tcgccctgag cgtggccggc gggctgatcg
      361 tcatcagcgt cgtcagccgc tgatcccagc gggataaatt gtcgacaatg tgcaaaccgg
      421 gggcatgcaa accctcgacg ccgccgccca acacgcccgc tacgtcctaa ccccctgggt
      481 cgcgcaaggc ggccgccggc cgccggcgct cgtccgcggc gagggatcgt acctttacga
      541 cgacgcggga aaacgctacc tcgacttctc gtcggggttg gtcgcggtca acctcgggca
      601 cggccatccg gccatcgcgc gcgcgattgc cgaacaagcc gaacggctgg catacgcctc
      661 gcccggcttc gtcctcgaca agcgcgccga gctcgccaaa gcgatcgtcg aaatcggccc
      721 ctgggaagag ggcgggcgca tcttcttcac caccggcggc ggcgaggcca acgaggacgc
      781 cgtcaagttt gcgcgcgcgc tgaccggccg gcacaaggtg ctgacggcgt accgttcctt
      841 ccacggctcg gcacccggcg ccggaacgct gaccggcgag aaccggcgct ggccgaacga
      901 acccggtatc ccgggcgtcg tgcatttttt tgcgcccttt ccgtaccgca gtccgttctt
      961 cacgcgcgac ccggccgaag aggtcgaacg cgcggtcgcg cacctcgaag agatcgtacg
     1021 ctacgaaggc ggcgaccgca tcgcggcgat cttgatcgaa ccggtggtcg gttccaacgg
     1081 cgtgatcgtg tatcccgacg gttacctggc acgtctgcgc gagctgtgcg accgacacgg
     1141 cattctgctg atcttcgacg aggtgatgac cggattcgga cgcaccggtg cggcatttgc
     1201 cgcgcagcgc ttcggcgtcg tacccgacct gtttacgttt gcgaaaggcg tgacgtcggc
     1261 atacgtgccg ctcggcggcg tcgcgctgcg cgaatcgctc gcagcgcatt tcgacacgcg
     1321 tcccctcccg agcggtcaca ctttcagcgg acatccggtc gccatggcgg ccggtcttgc
     1381 gacggtgacg gcctatcgtg aagagcggac tttcgaccgc gcgcgcgccc tcgagcccga
     1441 gctgcgccgg cgtttcgagg agctgcaacg ccgccatccc ggcgtcgtcg gcgaggtgcg
     1501 cggcgtcggt gcgttcttcg gcctcgagct cgtatccgat ccccaaactc gccgtccgct
     1561 ggtcgattgg caaggaacgg cgagcctgca aacgtttttc gacgaccttc tgcagcgcgg
     1621 tctctacgcg ttcggacgtt acaacgtcgc gatcctcgcc ccgccgctca acgcaaatgc
     1681 cggcgaactc gacgaagcgg ccgcgatctt cgacgccgcg ctcggaaaac tcggaggtac
     1741 tcgatgacga ccgacctttc ggttcgcaac cccgcgacgc gcgaaatcat cggccgcgtt
     1801 cccgtggccg gcagccggga cgtcgacgcg gccgttgcgg cggcgctcgg cgcgttcgac
     1861 tcgtggtcga aggttccggt cgtcgaacgg gcgcgcttga tgttccgatt tgccgacgcg
     1921 ctcgaacgtc gccgcaacga cctggcgctt tcggtatcgc gcgaaaacgg caagacgctg
     1981 gccgacgccc gcgccgaggt acggcgaggg atcgaagccg tcgagttcgc gtgcgggatg
     2041 ccgtcgctgc tgatgggcga cgcgctgccc gacgtcgcac gcggaatcga ctccgtgtcg
     2101 gtccgttacc cgctgggcgt gtgcgcgggc atcacgccgt tcaattttcc ggcgatgatt
     2161 ccgctgtgga tgctgccgat cgcggtagcg gccggcaacg cgttcgttct caagccgtcg
     2221 ccgcagacgc cgctcacggc cgaactcctc gcggagacgg cgcgcgagtg ccgcttcccg
     2281 gacggtttgt tcagcgtggt tcacggcggg gcggaaaccg tcgacgcgct gatcgaccat
     2341 ccgggcatcg cggccgtctc gttcgtcggc tcctcgaagg tcgcgcgtct cgtgcaagag
     2401 cgcgcggtcc gcgcgcacaa gcgcgtgcaa gcgctcggag gcgccaagaa ctacttagtg
     2461 gtgatgcccg acggcgttac gcgggcgacc gtggatgcgg tgatttcctc ggccttcggc
     2521 ggtgccggcc agcgctgttt ggcgggctcc gtcgccgtag ccgtcggcga ggccggcgac
     2581 cggttcgtcc cgatgctcgt cgaagcggcg cgcgcgctgc gtttgggtcc cggcaccgac
     2641 gaggacgtcg acatgggacc ggtcgtcagc gacgaggcgt ttgcgcgcgt cgaaggctac
     2701 gtcgcacgcg cgcgcgccgc cggcgagcgc gtgcttctcg agccggcggc ttcacccgcg
     2761 accggatcgt ttctctcgcc ggccatcttc gattccgtaa gcccatcatc ggaactggtt
     2821 cgcgaggaga tcttcgggcc cgtgctggcg gtcgtccggg ccgcgacgct cgacgaagcg
     2881 atcgcgcttg ccaacgcttc gcgctacgga aacgcgtcgt cgatcttcac cgcgagcggc
     2941 gccgccgcgc gtacctttgc atcgaggatc gccgtgggga tggtcgggat caacgtcggc
     3001 gtcgccgctc cgatggcgtt cttccccttc ggcggcgtca aggattcgat tttcggcgat
     3061 ctgcgctgtc acggaaagga tgcggtcgca ttctacacgc agcagcgagt cgtaatgagc
     3121 cgttgggccg agccgagttg aacgaaccgc ccgccctccg ctccggatcg ctgacgttct
     3181 tggaagtgct cgcgacgtcg gtggcgctga tcggccctag catgacgccg attctcatcg
     3241 cgccctacat gttcgccaat gccggcaacg gcacgtggct ggcgtacgca ttcggcggga
     3301 cgatgctggt gttcgtcgcg ctcaacatca atcagttcgc gcggcggtcg agcgaagccg
     3361 gttccatgta cggctacgtc gccgccaatc tcggaccgat gctgggtgcg atcgccggct
     3421 ggtcgctgct gtgggcgtac ggtttcgtcg cgtcggccgt tctgggcgcg atggcgctct
     3481 tcgtcgagct gcttctcggc tacggcaacc tgcacgcccc cgccgtggcg atcgtgtgcg
     3541 cgatcgcggt cgtcgcctgg caggcggcct accgcggagt tcaggtatcg gcgatcgtga
     3601 tgctcgtgct cgaagcgatc tcggtaacgg tgatctgcgt actgatcggg atcgtgctgg
     3661 tcaaacacgg accgtcgctc gacgtgagcc aagtgaagat cgcgggcgga ttcccgggcg
     3721 ggatcgggct ggcgattatg ttcgcggtgt tcagcttcgt cggcttcgag agcgcgaccg
     3781 cctttggcgc cgaagcccgc aacccgctgg tgacgatccc gcgggccgtg atcggcagcg
     3841 tcgttttcgt cagcatcttc ttcatcgtcg cgacctacgc cgaagtgctc ggcttgcgcg
     3901 gcgcgccgat cccgttggac cagcaaacct ttccgctcgg aacgctcgca aacgtgttcg
     3961 acgttggata tctccggatt ccgatcgcca tcggagccct cgcgagcgcg ttttcggtgt
     4021 gcctcgcctg catcaccacc gccggccgcg tggcgtacgc catggcggtg tccggcctgc
     4081 tgccgccgtc gttcgcgcgc atcgaaccgc ggcacgacac gccgaacgtc gcggtaac
//
LOCUS       DASUCJ010000580         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_235230, whole genome shotgun sequence.
ACCESSION   DASUCJ010000580 DASUCJ010000000
VERSION     DASUCJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235230"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..189)
                     /locus_tag="VFU90_06880"
     CDS             complement(28..189)
                     /locus_tag="VFU90_06880"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU5479540.1"
                     /translation="MGAILWAIIVVLVILWLLGFLAFHIAGGLIHILIVVAVVLLIVN
                     LLTGRGARV"
     gene            complement(238..1323)
                     /locus_tag="VFU90_06885"
     CDS             complement(238..1323)
                     /locus_tag="VFU90_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479541.1"
                     /translation="MLSPKPRTPARAFGDRFGARIERLGLVLEPDGNPDEAAGILNPA
                     AARTPGGELLLYPRCVASGNVSRVGTVLATANERGFDVRRLDYALVPQAEYEIRPHPG
                     YGCEDPRVTYVPVLGAYLMAYTAFGPEGPRIAIALSEDAYRWTRLGLVRFDEAGVIIG
                     DDKDAAFFPEPVISPSGRESLAFYHRPMLHLSAVDGRAAIPMIERMPFEDRESIRIAY
                     VPLDAVLKDRRNLLAVAESVQVLSPDANWGSIKIGAGTPPVRIAEGWLSLFHGVDVIE
                     GPGSKPALSYSAGAVVHDLERPDRILYRSPKPILTPTTERERVGIVDNVVFPTGLDPR
                     PDLGERTFDVFYGMGDHSIGAARMHVD"
     gene            complement(1364..1795)
                     /locus_tag="VFU90_06890"
     CDS             complement(1364..1795)
                     /locus_tag="VFU90_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479542.1"
                     /translation="MKRWWALAALLLLVACREVRVQTLAQGTTTALGDDQIIVVRNPY
                     DLAKLGIHAPVHFRGEFGVILLMGPHARTGYKQIIESIRANDQRVRVVAFEQEPADGG
                     EPSAAYRTYTLWIVPNSVYRRGVHVEVVTPSDQPIASTFLP"
     gene            1933..2853
                     /locus_tag="VFU90_06895"
     CDS             1933..2853
                     /locus_tag="VFU90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU5479543.1"
                     /translation="MNTLRRLALGADLVALGTVMLGSWTRINGAGETCPDWPLCHGSV
                     VPAMSGGTLWEWMHRLLAFLVAPLVVALIAVAWRKRHESPFIAPTVVVIAVLFLAQVL
                     LGAATVQLANAPISVVLHWGTAMAFIAALSAMAIFAAAAGDGRVRTPQATSSFSVGAV
                     LCVTAVSAFATMCVGAYVSSSGAGLACLSLPGCAGNVVVYTDGQYVQMVHRIAAGITL
                     LCAAGSLAVAWARPASGRVRIAVTAGVSLVCLQVLLGLLNVALRLPTDLREAHAANAA
                     LTFLAFVAAAVFAALDANLVRAPERLEANR"
     gene            2850..3728
                     /locus_tag="VFU90_06900"
     CDS             2850..3728
                     /locus_tag="VFU90_06900"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU5479544.1"
                     /translation="MSALAARVGSYYELSKPRIILLLLITTAAAMLMAARGLPPLALA
                     FWTLLGGALSAASAAALNCAYDADIDGLMKRTSARPIPQGRVSVHDAVLYAVAVGAAG
                     FAILYFLVNPLAAWLSLAGNVYYVLIYSIWLKRLTPLNIVIGGAAGAIPPLVGWAAVT
                     HAIGGPALGLFALIFLWTPPHFWSLSLMTELDYAKAKVPMLPNVYGERRTKLEIVYYS
                     AILVAASLALYPLHVMGALYFAAAAVLGGIFMLDAIRTLRERDGNLWARRLFRFSLLY
                     LALMCAIMVIDRIVLS"
     gene            3725..>4059
                     /locus_tag="VFU90_06905"
     CDS             3725..>4059
                     /locus_tag="VFU90_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479545.1"
                     /translation="MKPLLLTLSLGVFAGALDLSVLAPALPALGREFGVATGDLAWVF
                     TLYLLVTVVSIAVASSLADRYGRRAVYLGCIALFAAGSILAIVAPSYVVFLSARALQA
                     LGAGGVFPVA"
BASE COUNT          590 a         1359 c         1343 g          767 t
ORIGIN      
        1 ggtgctcgcg cgctagcggc ttcggcgcta gacgcgcgcc ccgcgtccgg tcagcaggtt
       61 gacgatcaac agcacgaccg caaccacgat caaaatgtgg atgaggccgc cggcgatgtg
      121 aaaggccagg aagcccaaca gccagaggat gaccagtacg acgatgatcg cccagagaat
      181 tgcgcccata gactttctcc tcgcagtgtc cggcgtgctg gagtgcctat acccgtttta
      241 gtcgacgtgc atgcgggcgg cgccgatgga atgatcgccc atcccgtaaa acacgtcgaa
      301 cgtcctctcg cccagatccg gccgcgggtc gaggccggtc ggaaatacga cgttgtcgac
      361 gattccgacg cgttcgcgct cggtcgtcgg cgtgagaatc ggtttgggcg accggtaaag
      421 aatgcggtcg ggccgctcga gatcgtgcac gaccgcaccc gcgctgtaac tcagcgccgg
      481 cttactgccg ggcccttcaa ttacgtccac gccgtggaac agcgacagcc agccttccgc
      541 aatccggacc ggtggagtcc ccgcgccgat ttttatcgat ccccagttgg cgtcgggcga
      601 gaggacttga acgctctcgg ccaccgcgag cagattgcgg cggtccttca gcaccgcatc
      661 gagcgggacg taggcgatgc gaatcgattc ccggtcctcg aacggcatgc gttcgatcat
      721 cggaatcgcg gcgcgcccgt cgaccgccga gagatgcagc atcggacggt gatagaatgc
      781 caagctttcg cgcccgctcg gcgagattac cggttccgga aagaaggctg cgtctttgtc
      841 gtcgccgatg atcacgccgg cctcgtcgaa acgaaccagc ccgagccgcg tccagcgata
      901 ggcatcttca gagagtgcaa tcgcgatccg cggaccttcg gggccaaacg ccgtgtacgc
      961 catgaggtac gcccccagca ccggcacgta cgtgacgcgc ggatcttcgc agccgtagcc
     1021 gggatgcggg cggatttcgt attcggcctg cggcacgagc gcgtagtcga gccgccgcac
     1081 gtcgaatccg cgctcgttcg cggttgcgag cacggtgccg acgcgcgaaa cgttgccgga
     1141 cgccacgcaa cgcggataca gcagcagctc gcccccgggg gtgcgggcgg cggccggatt
     1201 gaggatcccg gcggcttcgt cggggttgcc gtcgggctcc agcacgaggc ccagccgctc
     1261 gattctcgca ccgaagcgat cgccgaatgc acgcgcgggc gtccggggtt tgggtgaaag
     1321 catcgctggt ggtttcgtcc gatctaggct cagcccgtct tttttatggc aggaaggtgc
     1381 tcgcgatcgg ctgatcgctc ggcgtgacga cctcgacgtg cacgccgcgc cggtacacgc
     1441 tgttggggac gatccaaagt gtgtaggtgc ggtaggccgc cgagggctcg ccgccgtcgg
     1501 ccggttcctg ttcgaaagcg accacgcgca cccgctgatc gttcgctcga atcgactcga
     1561 tgatctgctt atacccggta cgggcgtgcg ggcccatcag gagaatcacc ccgaactcgc
     1621 cgcggaagtg caccggcgcg tgaatgccga gcttcgccaa gtcgtatggg tttcgcacga
     1681 cgatgatctg gtcgtcacca agcgcggtcg tcgtgccctg tgcgagcgtc tgcactcgga
     1741 cctcccggca ggcgaccagg agcaagagcg cagccagcgc ccaccagcgt ttcacggccg
     1801 acggcttcgc ccgcgcgggc ttgccgcccc ccaatgcaac ggccgatttt cggccgcgcc
     1861 aaggctgtat cgcgtcaagc ctccaatgtc attgtgaccg atgctcctcg cacgctacgc
     1921 ttttagccgt tcatgaacac tctgcgccgt ctcgcgctgg gagccgatct cgtcgcgctc
     1981 gggaccgtca tgctgggaag ctggacgcgc atcaacggcg ccggcgagac ctgcccggat
     2041 tggccgctct gccacggatc ggtcgtcccc gcgatgtccg gcggaacgct ttgggagtgg
     2101 atgcaccgcc tgctggcatt cttggtcgcg ccgctggtcg tcgccctgat cgccgtcgct
     2161 tggagaaagc gtcacgagtc gccgttcatc gcccccacgg tcgtcgtcat cgcggtcttg
     2221 tttctggcac aggtcctgct cggcgcggcg accgtccagc tcgccaacgc gccgatttcc
     2281 gtcgtactgc attggggaac cgcgatggcg ttcatcgcgg cgctttcggc gatggccatc
     2341 ttcgcggccg ccgccggcga cggccgcgtg cggacgcccc aagcgacgtc gtcgttctcg
     2401 gtcggtgcgg tgctgtgcgt taccgccgtc tcggcgtttg cgacgatgtg cgtcggcgcg
     2461 tacgtcagct cgagcggcgc cggtctcgcg tgcctttcgc ttcccgggtg cgcgggtaac
     2521 gtggtcgtct acaccgacgg ccagtacgtc cagatggtgc atcgcatcgc cgccgggatc
     2581 acgctgctgt gcgccgccgg gtcgctggcg gtggcgtggg cgcggccggc gtccggccgc
     2641 gttcgcatag ccgtcacggc cggcgtttcg ctcgtgtgcc tgcaagtgct gctcggcttg
     2701 ctgaacgttg cgctgcgcct gccgaccgat ctgcgcgaag cacacgcagc caacgccgcg
     2761 ctgacctttc tggcgttcgt cgccgcagcg gtgttcgcgg cgctcgatgc caacctcgtg
     2821 cgcgcgcccg aacggctcga ggcgaaccgg tgagcgcgct ggcggcgcgc gtcggaagct
     2881 attacgagct ttcgaaaccg cggattattt tgctgctgct gatcacgacg gccgccgcga
     2941 tgctgatggc cgcgcgcggc cttccgccgc tcgcgctcgc gttctggacg ctgctcggcg
     3001 gagcgctttc ggccgcctcg gccgccgcgc tgaactgcgc gtacgacgca gacatcgacg
     3061 gcttgatgaa acgcaccagc gcgcgcccga tcccgcaagg acgagtttcg gtccacgacg
     3121 ccgtgctcta cgccgtcgcc gtcggcgcgg ccggctttgc gattctctat tttctcgtca
     3181 atccgctggc ggcgtggctg tcgcttgccg gaaacgtgta ctacgtgctg atctattcga
     3241 tttggctgaa gcgtttgacg ccgctcaaca tcgtcatcgg cggggcggcc ggggcgatcc
     3301 cgccgctggt cggctgggcc gccgtgacgc acgccatcgg cggaccggca ctcggtttgt
     3361 tcgcgctgat tttcctgtgg acgccgcccc acttctggtc gctgtcgctg atgaccgagc
     3421 tcgactacgc caaggccaag gtgccgatgc ttcccaacgt gtacggcgag cgccgcacca
     3481 agctcgaaat cgtgtactac agcgcgatcc tagtcgccgc gtcgctggcg ctctacccgc
     3541 tgcacgtgat gggggcgctg tatttcgccg cggcggcggt attgggcggc atcttcatgc
     3601 tcgacgcaat tcgcacgctg cgcgaacgcg acggaaacct ttgggcgcgg cgcctcttcc
     3661 gtttctcgct gctctatctc gcgctgatgt gcgcgatcat ggtgatcgac cgcatcgtcc
     3721 tctcgtgaaa ccgctactcc tcacgctgtc gctgggcgtc tttgccggcg cgctcgactt
     3781 gagcgtgctc gcgcccgcgc tgcccgcact gggacgggaa ttcggcgtag cgacgggcga
     3841 cctcgcctgg gtcttcacgc tgtatctgct ggtgaccgtc gtttcgatcg cggtggcgag
     3901 cagcttggcg gaccgctacg gacggcgggc ggtgtatctc ggctgcatcg cgctgttcgc
     3961 ggcggggagc atcctggcca tcgtcgcacc gagctacgtc gtctttttgt cggcccgcgc
     4021 gctgcaagcg ctcggtgcgg gcggagtttt cccggtagc
//
LOCUS       DASUCJ010000581         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_144040, whole genome shotgun sequence.
ACCESSION   DASUCJ010000581 DASUCJ010000000
VERSION     DASUCJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144040"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFU90_06910"
     CDS             complement(<1..544)
                     /locus_tag="VFU90_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEU5479546.1"
                     /translation="MFDELSEKLDAVFARLRGRGVLSEADIKEGLREIRRVLLEADVN
                     FQLTREFLERVEQKAVGVSALRTVSPGQQLVKIVHDELTAMLGEQREGLRMSTVPPTI
                     VLMVGLQGSGKTTTAAKLARRLAGEGRPTRLVAADVYRPAAIDQLETLGQQIGAPVTL
                     DRSTTDVVKIAKKGVDEARRA"
     gene            687..>1155
                     /locus_tag="VFU90_06915"
     CDS             687..>1155
                     /locus_tag="VFU90_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5479547.1"
                     /translation="MARAQRKEEILRSELPPTLPLMALRSTIVYPLGTIAVQMGAPEN
                     LSIIRSNDEPGLIVGLLVATGDHDEPINADAFVGRVGVAARVHERINLPGDTIQITLQ
                     GLRRIVVESVVSREPYPVAVIKPAKETPLDPIEGEELMARLVAAAETLAELVDR"
BASE COUNT          187 a          369 c          395 g          204 t
ORIGIN      
        1 gcgcgcgccg cgcttcgtcg acgccctttt tggcgatctt gacgacgtcg gtggtggacc
       61 ggtcgagcgt gaccggcgcg ccgatctgct gtcccagcgt ttcgagctga tcgatggcgg
      121 cggggcgata gacgtcggcc gcgaccaacc gagttgggcg gccctcgccc gccaagcggc
      181 gcgcgagctt ggcggcggtc gtggttttcc ctgaaccctg caggccgacc atgagcacga
      241 tggtgggcgg cacggtgctc atgcgcaggc cttcgcgctg ttcgcccaac attgcggtca
      301 gctcgtcgtg gacgatcttg acgagttgct gtccgggcga gacagtgcgg agggcagaga
      361 cgccgacggc tttctgctcg acgcgctcga ggaattcgcg ggtgagttgg aaattgacgt
      421 cggcttcgag gaggacgcgg cggatctcgc gcagtccttc cttgatgtcg gcttccgaca
      481 ggacgccgcg gccgcgcaga cgcgcgaaca ccgcgtccag cttctcactc agctcgtcaa
      541 acatagccgt gaaagataga tcgacgtatc attgagttca acgacgccgc gacgatcgag
      601 gcccttgatc gtgtcccacc gcggcgccgg ccggatgcca gcaagccact cgccgtcttc
      661 ttcgacgtct ccctcactca accgaaatgg ctcgcgcaca gcgtaaggaa gagattcttc
      721 gctcggagct cccgccgacg ttgccgctca tggcgcttcg gtcgacgatc gtctatccgc
      781 tgggcaccat cgccgtgcag atgggcgcgc cggagaacct gtccatcatc cggtccaacg
      841 atgagcccgg attgatcgtg ggactgctcg tggcgacggg cgatcacgac gaaccgatca
      901 atgccgacgc gttcgtcggc cgggtgggcg tcgcggcgcg cgtgcacgag cgcatcaatc
      961 tgccgggcga cacgattcag atcaccctcc agggactgcg gcgcatcgtg gtcgagtcgg
     1021 tggtgtcgcg cgagccgtat ccggtggccg tgatcaaacc ggcgaaagag acgccgctgg
     1081 atccgatcga gggcgaggag ctgatggcgc gcctcgtcgc cgcggccgag acgctggccg
     1141 agctggtgga tcgca
//
LOCUS       DASUCJ010000582         1074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1091301, whole genome shotgun sequence.
ACCESSION   DASUCJ010000582 DASUCJ010000000
VERSION     DASUCJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFU90_06920"
     CDS             complement(<1..1034)
                     /locus_tag="VFU90_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479548.1"
                     /translation="MHRLLRDGTRVLTLLALCACGGTESPTLSTPVKIVAISGGGQAA
                     DLGQPLAQPLVARVTTSTGTPASAVSVTFAVTSGAGTLSPATVSTDGSGNASTTFTMG
                     QTPGVTTVSATSGAVAGAAADFIVSTGPTIVVHVALAAGNVRLPALSHGPLRAPVVMP
                     GIVPRPIKGLQLDVAYRPSAIGVPGPGAIRSLGLDQARVIAASIRSRLTTMPIASRFE
                     VMAVSPAIAVARLRVHAASDRDSVMRALRADPAVASVSVDGLVSRGPIAKSAFTPVSH
                     GRPRASANPGAGTIMFPATQGMDAQLWNYTLIDAPRAWHVVTGSPSVVVAVIDDGINQ
                     HPDIAANLDMS"
BASE COUNT          182 a          363 c          382 g          147 t
ORIGIN      
        1 gacatatcga ggttggccgc gatgtcgggg tgttgattga ttccgtcgtc gatcacggcc
       61 accacgacgc tcggactccc ggtcacgacg tgccaggcgc gcggcgcatc gatgagagtg
      121 tagttccaga gttgggcatc catgccctgg gtcgcgggga acatgatcgt gccggcgccc
      181 ggattagctg acgcacgcgg ccgcccgtgc gacacgggtg tgaacgcgct ctttgcaatg
      241 gggccgcggc tcacgagacc atccacgctc accgacgcga cggccggatc ggcacgcagt
      301 gcgcgcatca cggagtcgcg gtcggatgcc gcatgcacgc gaaggcgcgc caccgcgatc
      361 gccggcgaca cggccatgac ctcgaagcgc gacgcgatcg gcatcgtcgt taggcgcgag
      421 cggatcgacg ccgcgatcac gcgcgcctgg tccaggccga gcgaccggat ggcaccgggg
      481 ccgggcacgc cgatcgccga ggggcgatac gccacgtcga gctgcagtcc cttgatgggg
      541 cgcggcacga taccgggcat caccaccggt gcgcggagcg gaccgtgcga gagcgcggga
      601 agccgcacgt tgcccgcggc gagcgcgacg tgcacaacga tcgtcggacc ggtgctcacg
      661 atgaaatccg cggccgcacc ggccacggcg ccgctcgtgg cgctcacggt cgtcacgccc
      721 ggcgtctggc ccatcgtgaa cgtcgtcgac gcgttgcccg acccatcggt gctaacggta
      781 gccggcgaca atgttccggc acccgacgtg accgcgaagg tcaccgatac ggcggacgcg
      841 ggtgtcccgg tcgaggtcgt gacgcgcgct accagcggct gcgccagcgg ctgtcccaga
      901 tcggccgcct ggccgccgcc cgaaatcgcg acgatcttga ccggtgtgga cagagtcggc
      961 gactccgttc cgccgcatgc gcagagtgcg agcagcgtga gaacgcgtgt cccgtcgcgg
     1021 agcaaccgat gcatcgacat gtgcaacacc agatgggcaa agcgaagacg ttgg
//
LOCUS       DASUCJ010000583         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_480032, whole genome shotgun sequence.
ACCESSION   DASUCJ010000583 DASUCJ010000000
VERSION     DASUCJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480032"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFU90_06925"
     CDS             complement(<1..574)
                     /locus_tag="VFU90_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479549.1"
                     /translation="MPWHISFPALFALFLFVVIPAAAAAGVHALFRRFVPATRLLPHQ
                     EVAGFLVAVVGVLYAVVLGFIVVTTWSAYDEAQRTADVEAGDVGDAFGFASMLPEPRR
                     GDMQRLLAQYAIEVRDREWQTMQHGREDLRARALLIDAARALGEPVVKPSRDLDEALN
                     RATTRTAVAASLRDIADNRRLRLIEAENGMQ"
     gene            665..>2695
                     /locus_tag="VFU90_06930"
     CDS             665..>2695
                     /locus_tag="VFU90_06930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5479550.1"
                     /translation="MKTTKILSWTGLLIALGATAAYAARPVVPEDLFKLTLLSNAAIS
                     PDGTHVLVVAAKANGPKNTYDRSIDLIDVSTGALTANVTGRTGDADYAWMPDSASFVF
                     VRTLPKGKPQLYRYVLASRKSVKLTQIKQGVSNPVVSHDGKHIALTVTDIDPPGSAHI
                     DFTKAGFKPSASQKQTDIRTTDALFFEANGQGDVYRDHAHIWTIQADGSNPKQLTSGK
                     WSEGFDAWSPDDRTIAFDSLRYDAVDSGPSDVYVVPASGGTPRKIASPQPANYGLFFN
                     GDGTRFFAFRAGVKDAAGLPALVSSQLDGSDARTVVAEDRLSWGDSLLADMKEGGGLC
                     AAPLPGGTRALMNLDGPGYANLRTVDLTTGALTDVTPPRGEAWSCTVSRDGRTATYLY
                     SDFLHPADVYVANLAAGTQRRLTDVNAAYLSSVALSTPQEFSVKDPAGNTVQAWFMPA
                     TAGPPGTKHPTILNIHGGPETQFGDTFFLEFQYYASLGYNVVFSDPAGSTGHGYAFEE
                     ALESNYGDAMFQDVQAVMDAAVQRPDVDASRLGVTGGSYGGYATLWVIAHTDRYKAAV
                     AERAVSFLQSENLAADFAGKNGLGGGYYTWGPPWDPASTLYAKFSPLTYVANVRTPLL
                     ILHADLDTRAPIDQTLQEFTALKILGRTVEYVAVPDENHDLSRTGSPIHRVER"
BASE COUNT          494 a          891 c          884 g          426 t
ORIGIN      
        1 gctgcatccc gttttcggcc tcgatcaggc gcaatcgccg gttgtcggcg atatcccgca
       61 ggctcgcggc gaccgccgtt cgcgtcgtcg cgcggttgag cgcttcgtcg agatcccggc
      121 tcggtttgac aaccggctcg ccgagcgcgc gagcggcgtc gatcagcagc gcgcgggcgc
      181 gcagatcctc gcggccgtgc tgcatcgtct gccactcgcg gtcgcggact tcgatggcgt
      241 attgcgcgag cagccgctgc atgtcgccgc gccgcggctc gggcagcatg ctcgcgaatc
      301 caaaggcgtc accgacgtcg cccgcctcga cgtcggcggt ccgttgcgct tcgtcgtagg
      361 cggaccacgt cgttacgacg atgaagccga gcacgacggc gtagagtacg ccgacgaccg
      421 ctaccagaaa tccggcgacc tcctgatgag gcaacagccg cgtggccggc acgaagcggc
      481 ggaacagagc gtgcacgccg gccgccgcgg ccgccgggat cacgacgaag agaaaaagcg
      541 cgaacagcgc cgggaacgag atgtgccaag gcatggcacg cggttcggca cgccgtagca
      601 aaagtcgttc tacgcgtccg cggccaccgg gaatagcgat tcggagcgcg gaaacgcagc
      661 cgccgtgaag acgacgaaga tcctcagttg gaccgggttg ctgattgcgc ttggcgcgac
      721 cgccgcctac gcggcgcggc ccgtggtacc cgaagacttg ttcaagctga cgctgttgag
      781 caacgccgcg atttctcccg acggtaccca cgtcttggta gtcgccgcaa aggcgaacgg
      841 tccgaaaaac acctacgatc gctcgatcga cttgatcgac gtgtcgaccg gtgcgctgac
      901 cgccaacgtg accggacgga ccggcgacgc cgattacgca tggatgcccg actcggcgag
      961 cttcgtcttc gttcgcacgt tgcccaaagg caagccgcag ctctaccggt acgtgctggc
     1021 gtcgcgcaaa tcggtgaaac tcacgcaaat caaacaaggg gtctcgaacc cggtcgtctc
     1081 gcacgacggc aagcacatcg cgcttaccgt caccgacatc gatccgcccg gtagcgcaca
     1141 catcgatttc accaaagccg gattcaaacc gtccgcttcg caaaaacaaa ccgacatccg
     1201 gacgaccgac gcgctgttct tcgaagcgaa cgggcaagga gacgtctatc gcgatcacgc
     1261 gcacatctgg acgatccaag ccgacggctc gaatcccaaa cagctgacct ccggaaaatg
     1321 gtcggaaggc ttcgacgcat ggtcgccgga cgatcgcacg atcgcgttcg actcgctgcg
     1381 ctacgacgcc gtcgattccg gacccagcga cgtgtacgtc gtgccggcgt caggcggtac
     1441 gccgaggaag atcgcgtcgc cgcagccggc caactacggg ctgtttttca acggcgacgg
     1501 aacgcgcttc tttgccttcc gcgccggcgt caaagacgcg gccggactcc cggcattggt
     1561 gtcgtcgcag ctcgacggga gcgacgcgcg caccgtcgtc gccgaagatc gcctttcgtg
     1621 gggcgacagt ttgctcgcgg acatgaagga aggcggcgga ctgtgcgccg cgccgctgcc
     1681 gggcggaacg cgcgcgctga tgaatctcga cggcccgggc tatgcgaacc tgcgcacggt
     1741 cgacctgacg acgggtgcgc tgaccgacgt tacgccgccg cgcggcgaag cgtggtcctg
     1801 cacggtgtcg cgcgacggcc ggacggcgac gtacctttat agcgactttc tgcacccggc
     1861 cgacgtgtac gtcgcaaacc tcgcggcggg gacgcaacgc cggctcaccg acgtgaacgc
     1921 cgcctacctg tcgtcggtcg cgctctcgac gccgcaggaa ttctcggtga aggatccggc
     1981 gggcaatacg gtccaggcgt ggttcatgcc ggcgacggcc gggccgccgg gaacgaaaca
     2041 tcccaccatc ctcaacattc acggcgggcc ggagacgcag ttcggcgaca cgttcttctt
     2101 agaatttcaa tactacgcgt cgctcggcta caacgtcgtt ttctcggatc cggccggcag
     2161 caccgggcac ggctacgcgt tcgaggaagc gctggaaagc aactacggcg acgccatgtt
     2221 tcaagacgtg caggcggtga tggacgctgc ggtccagcgg ccggacgtcg acgcgtcgcg
     2281 gctcggcgtg accggcggct cgtacggcgg gtacgcgacg ctgtgggtga tcgcgcatac
     2341 ggatcgctac aaggccgccg tggccgaacg tgccgtgagc ttcctgcaaa gcgagaacct
     2401 cgcggcggat ttcgccggga agaatggcct gggcggcggg tactacacgt ggggaccgcc
     2461 gtgggatccg gccagcacgc tctacgccaa gttctcgccg ctgacgtacg tggcaaacgt
     2521 gcgtacgccg ctgcttatcc tgcacgcgga tctcgatacg cgcgcgccga tcgatcagac
     2581 gttgcaggag ttcaccgcgc tgaagatact gggccgcacg gtcgagtacg tggcggtgcc
     2641 cgacgaaaac cacgatctct cgcgcacggg ttcgccgatt catcgcgtgg agcgg
//
LOCUS       DASUCJ010000584         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1229091, whole genome shotgun sequence.
ACCESSION   DASUCJ010000584 DASUCJ010000000
VERSION     DASUCJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229091"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFU90_06935"
     CDS             complement(<1..722)
                     /locus_tag="VFU90_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5479551.1"
                     /translation="MSTPLPRREFAVTERFVYLNHAAVGVLPACSVEAIEGYVRAHAR
                     EGVLGTHPYDARMPEYRERLARFIGASGGELAIVPSTSAGANVVALGIDWRPGDEVLL
                     CDNEFPSNAVPWTALQRRGVSVRWISAPARRLTPDVLRDALAPQTRAVAVSWVSYSDG
                     YRHDLAALSEIAHAAGALLCVDAIQGLGAFPLDVRRCGIDALYAGAGKWLLGLHGAGL
                     LYVSEPLIERFELAAPGWRSME"
     gene            complement(719..1366)
                     /gene="phoU"
                     /locus_tag="VFU90_06940"
     CDS             complement(719..1366)
                     /gene="phoU"
                     /locus_tag="VFU90_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5479552.1"
                     /translation="MGEYVRIAYHEALEGTRLDVVRLGALVGDAIRLAVDALNSRDSA
                     AGARVVAGDDVIDDLRRRIEHHCIELIWRQQPVAGELREIAAMLEIATDLERVGDYAV
                     DIAKNAIKLSDQPMRPQKVEIDRIAGVAHAMLLDVMRAYTERNATLASEVIDRDDEVD
                     KLYKRSIKLLQEEMRADPEIVRPGTRYLFVLASLERVGDRAGNIAWHTKDMIGAE"
     gene            1483..2337
                     /locus_tag="VFU90_06945"
     CDS             1483..2337
                     /locus_tag="VFU90_06945"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU5479553.1"
                     /translation="MSEIRTVGVCGAGLMGNGIAQTCAMAGFDVVLVEVEQAPLDRGM
                     SAISKSLDKFAEKGKLSHADRDAAGKRIRATTSIGDLKACDLVIEAVVENAGVKKELF
                     RQLDELLAPHAIVCTNTSSLCVIELAAATRRPDRVAGLHFFNPVPIMKLVEVVKTIAT
                     SQATVDALFAFSKRLGKEPILAQDTPGFVVNRLLVPYLLYAIRLLEQGLASKEDIDKG
                     MKLGCGYPMGPFELLDFVGLDTTYYIAEIMFDEFKDPAMAAPPLLKRMVLAGRLGRKS
                     GKGFYDYA"
     gene            2330..>3086
                     /locus_tag="VFU90_06950"
     CDS             2330..>3086
                     /locus_tag="VFU90_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5479554.1"
                     /translation="MPDAAPYRDILVEIDGPVGIVTLNRPNVLNALRTGLLAELSDAL
                     SDLERNPGVLAIVLTGAGEKAFAAGADISELNALESAGAGADQARTGQALTRKIERLR
                     KPVIAAVNGFALGGGCELAMACDIRIASENAKFGQPEVNLGLIPGYGGSQRTTRLTGK
                     GMALYLCLTGEIIDAHEALRIGLVERVVPLADLMTEAKRIAGVIASKAPLAITACKRA
                     IHDGAHLPIDDALALEALEFGALVDTQDIKEGTG"
BASE COUNT          548 a         1025 c          987 g          526 t
ORIGIN      
        1 ttttccatcg agcgccatcc cggagcggcc agctcgaagc gttcgatcaa cggctcgctc
       61 acgtacagta atcccgcgcc gtgcagtccg agcagccatt ttcccgcgcc cgcgtagagc
      121 gcgtcgatgc cgcatcggcg gacgtccagc gggaaggcgc ccagcccctg aatcgcgtcg
      181 acgcagagca gcgcgccggc cgcgtgtgcg atctcgctca gcgccgcgag atcgtgccgg
      241 tatccgtccg aataggacac ccacgagacc gcaaccgcgc gcgtctgcgg cgcgagcgca
      301 tcgcgcaaaa cgtccggagt gagccgccgc gcgggtgcgg aaatccagcg aaccgacacc
      361 ccgcggcgct gcagcgccgt ccacggtacc gcgttggatg gaaactcgtt gtcgcacaac
      421 agcacttcgt cgccgggccg ccaatcgatg cccagcgcta ccacgttggc gcccgcgctc
      481 gtgctgggga cgatcgcgag ctcgccgccg ctcgcgccga tgaatcgcgc cagacgctcg
      541 cgatattccg gcatccgggc gtcgtacgga tgcgttccca gcacgccttc gcgcgcgtgc
      601 gcgcgcacgt acccttcgat ggcttcgacc gagcatgcgg gaagcacgcc gacggcggcg
      661 tggttgaggt agacgaagcg ttcggtgacg gcgaattcgc gacgcgggag cggcgtgctc
      721 actcggcgcc gatcatgtcc ttcgtgtgcc aggcgatgtt gccggcacgg tcgccgacgc
      781 gctcgagcga cgccaggacg aacaggtacc gcgtcccggg ccggacgatc tccgggtcgg
      841 cgcgcatttc ctcttgcagc agcttgatgc tgcgcttgta gagcttgtcg acctcgtcgt
      901 cgcggtcgat gacctcgctt gcgagcgtcg cgttgcgctc ggtatacgcg cgcatcacgt
      961 cgagcagcat cgcgtgcgcc acgcccgcga tccgatcgat ctcgaccttc tgcggccgca
     1021 tcggctgatc ggaaagcttg atcgcgttct tggcgatgtc cacggcgtag tcgccgacgc
     1081 gctcgagatc ggtcgcaatc tcgagcatcg cggcgatttc acgcagctct ccggctaccg
     1141 gctgctgtcg ccaaatcagc tcgatgcagt ggtgctcgat ccggcggcgc aaatcgtcga
     1201 tcacgtcgtc gcccgcgacc acgcgagcgc ccgccgcgga atcgcggctg ttgagggcgt
     1261 cgacggccaa gcggattgcg tcgccgacta acgcgccgag ccgaacgacg tcgagccgcg
     1321 ttccttccaa agcctcgtga taggcgatcc gcacgtattc tcccatctgc agagcgggcg
     1381 cccctagtat agggcaaagg taacttcccc gcaacgcaac gaatggacct gcgcccttta
     1441 tcaaaccata aggtagtccg aagcttccac aggagaacgt tcgtgtctga aattcgcacc
     1501 gtcggcgtct gcggcgccgg cctgatggga aacggtatcg cgcaaacgtg cgcgatggcc
     1561 ggtttcgacg tcgtgctcgt cgaggtcgag caggcgccgt tggatcgcgg tatgtcggct
     1621 atttccaagt cactcgataa gttcgccgaa aaaggcaagc tctctcacgc cgatcgcgac
     1681 gccgccggca agcgcatccg agcgaccacg tcgatcggcg atctgaaggc ttgcgatctc
     1741 gtcatcgaag cggtcgtcga aaacgccggc gtaaaaaaag agctcttccg tcagctcgac
     1801 gagctgctcg cgccgcacgc catcgtctgc acgaacacgt ccagcctatg cgtcatcgag
     1861 ttggctgcgg cgacccggcg ccccgaccgg gttgcgggat tacacttctt caacccggtg
     1921 cccatcatga agctcgtcga agtcgtgaag acgatcgcga cgtcgcaggc gacggtcgac
     1981 gcgctctttg cgttttcgaa acgtcttggg aaagagccga tcctcgcgca agatacgccc
     2041 gggttcgtcg tcaaccgttt gctggtaccg tacctgttgt acgcgatccg gctgctggag
     2101 caggggctcg cgtcgaaaga ggacatcgac aaggggatga agctcggctg cggctatccg
     2161 atggggccgt tcgagctgct cgacttcgtc ggactcgaca cgacctatta catcgccgaa
     2221 atcatgttcg acgagttcaa ggatcccgcg atggcggcac cgccgctgct caaacgcatg
     2281 gtcctcgccg gacgcctcgg gcgcaagagc ggaaaggggt tctacgacta tgcctgacgc
     2341 cgcaccctat cgagacatcc tcgtcgaaat cgacggaccg gttggaatcg ttacgttgaa
     2401 tcggccgaac gttctcaacg cgctgcgcac cggtttgctc gccgaactct cggacgcgct
     2461 ctcggacctc gagcgaaacc ccggcgtgct ggcgatcgtg ctcaccggtg cgggtgaaaa
     2521 agcgtttgcg gcgggagccg atatctccga gctcaacgcc ctcgaaagcg ccggcgccgg
     2581 cgcggatcag gcccgcacgg gtcaggcgct gacccgcaag atcgagcgct tgcgcaaacc
     2641 ggtgatcgcg gccgtcaacg gcttcgcgct gggcggcggc tgcgagcttg cgatggcctg
     2701 cgacatccgg atcgcttcgg agaacgccaa attcggtcag cccgaagtca atctcgggct
     2761 gattccgggt tacggcggct cgcagcgcac gacgcgcctg accggcaagg gaatggcgct
     2821 gtacctgtgc ctgaccggcg aaattatcga tgcgcacgag gcgctgcgga tcggactggt
     2881 cgagagggtc gtgccgctcg ccgatctgat gacggaggcg aaacggatcg ccggcgtcat
     2941 cgcctccaag gctccgcttg cgatcaccgc ctgcaagcgc gcgatccacg acggcgcgca
     3001 cctgccgatc gacgacgcgc tcgcgctcga agcgctcgaa ttcggggcgc tcgtcgacac
     3061 gcaggacatc aaagaaggca ccggcg
//
LOCUS       DASUCJ010000585         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_235296, whole genome shotgun sequence.
ACCESSION   DASUCJ010000585 DASUCJ010000000
VERSION     DASUCJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235296"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1224)
                     /locus_tag="VFU90_06955"
     CDS             complement(25..1224)
                     /locus_tag="VFU90_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5479555.1"
                     /translation="MTAQEQTKTETLDLALIGNSCAAALVNRNARIVWWCFPLFDSDP
                     VFSRLIAGDEEKGFCDITLAERVETESRYMRNTAIVETVLTDANGSKVRVSDFAPNFD
                     HYERVYRPPQLIRRIEPIAGLPRITIRVRPTHHYGCPITSFVFGSNHIRYMGGGQVLR
                     LTTDAPLSYIVNEISFPLTRPLNLIFGVDDPFLSAIDTTSREFLDRTRDYWHTWVRNL
                     ALAYEWQAEVVRAAITLKLCSFAETGAIIAAHTTSLPEARSSPRTWDYRYCWLRDAYF
                     VVDALNRLGATQIMESYIHYITTIATIGEDIRPVHGIVPYASLEETTATDLKGFLGHG
                     PVRVGNQAADQIQNDVYGSVVLAATQMFVDERLPKMGDEALFRLLEGLGEHAITRAFE
                     PDAGLWE"
     gene            complement(1221..>1812)
                     /gene="otsB"
                     /locus_tag="VFU90_06960"
     CDS             complement(1221..>1812)
                     /gene="otsB"
                     /locus_tag="VFU90_06960"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU5479556.1"
                     /translation="ALISGRPIDELDRLFEPLRLRASGVHGAEMRFDPGLPPTSASAA
                     RALPQSLWADLTRIAAAFPGAFVENKRFSFTVHYRLALESERRLREAVLRLIDSSQIP
                     VEVMSAHYAIELKPPGCDKGGAIAAFLSAPPFRGRTPVFVGDDTTDESGFAVVAARGG
                     YAFSVGKRRPGAIGTFAEPSAVRGWLAEFVGRGGGE"
BASE COUNT          335 a          560 c          608 g          309 t
ORIGIN      
        1 ggtgtgggcg cgcgagcgct tgcgttattc ccacaaaccg gcgtcgggct cgaaagcgcg
       61 ggttatggcg tgttcgccga gaccctcgag gaggcggaac agcgcctcgt ctcccatttt
      121 cggcagccgt tcgtcgacga acatctgcgt cgcggcaagc acgacgctgc catagacgtc
      181 gttttgaatt tgatccgccg cctgattgcc gacccgcacc gggccgtggc caaggaagcc
      241 cttgagatcg gtcgctgtgg tttcctcgag cgaagcgtac ggcacgattc catgcacggg
      301 gcggatgtcc tcgccgatcg tggcgatcgt ggtgatatag tgaatatacg attccatgat
      361 ctgcgtggcg ccgagccggt tcagcgcgtc gacgacgaaa taggcgtccc tcagccagca
      421 gtagcgatag tcccaggtgc gcggcgacga ccgggcctcg ggcagggacg tggtgtgcgc
      481 ggcgatgatg gcgccggttt ccgcgaagct gcagagcttg agcgtgatcg ccgcgcgcac
      541 cacttcggcc tgccattcgt aagcgagcgc gaggttgcgc acccaggtgt gccaatagtc
      601 gcgggtgcgg tcgagaaact cccggctggt ggtgtcgatc gcgctgagga aggggtcgtc
      661 cacgccgaag atgaggttga gcggccgggt cagcgggaag gaaatctcgt tgacaatgta
      721 cgagagcggg gcgtcggtcg tcaggcgcag aacctgtccg ccgcccatgt atcggatgtg
      781 gttggagccg aagacgaaac tggtgatcgg acacccatag tggtgggttg ggcgcacgcg
      841 aatcgttatg cggggcaggc ccgcgatcgg ctcgatgcga cgaatgagct gcggcggccg
      901 gtacacgcgc tcgtaatgat cgaagtttgg cgcgaaatcc gagacgcgca ccttcgagcc
      961 gttcgcatcc gtcagaaccg tctcgacgat ggccgtgttg cgcatgtacc gagactcggt
     1021 ttcgactcgc tccgcgagcg tgatgtcgca gaatcctttc tcctcgtcgc cggcgatgag
     1081 ccgcgaaaac acgggatcgg aatcgaacag cggaaagcac caccatacga tgcgggcgtt
     1141 gcgattgacg agcgccgccg cgcaactgtt gccgatcagc gccagatcca aagtctcggt
     1201 tttcgtctgc tcttgcgcgg tcattcgccg ccaccgcgac cgacgaattc ggcgagccag
     1261 cctcggaccg cgctcggctc cgcgaacgtc ccgatcgcgc ccggccgccg cttgcccacg
     1321 gaaaaggcgt agcctccccg cgcggcgacg acggcaaagc cgctttcgtc ggtcgtatcg
     1381 tcgccgacaa agaccggcgt ccggccgcgg aatggcggcg cggaaagaaa cgcggcgatg
     1441 gcgccgccct tgtcgcaacc cggcggcttc agctcaatcg cataatgcgc gctcatcact
     1501 tcaaccggga tttgggaaga atcgatgagc cgaagaacgg cctcccgcag acgccgctcc
     1561 gattcaagcg ccaggcggta atgaacagtg aaactgaagc gcttgttctc gacgaacgcg
     1621 cctgggaacg ccgcggcgat ccgggtcaga tccgcccaaa gcgattgtgg caacgccctc
     1681 gccgcggagg ccgatgtcgg cggcaagccc ggatcgaagc gcatttcagc tccgtggacc
     1741 ccgcttgcac ggagacgcag cggctcgaac aggcggtcga gctcatcgat gggacggccg
     1801 ctgatcagcg cc
//
LOCUS       DASUCJ010000586         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_831458, whole genome shotgun sequence.
ACCESSION   DASUCJ010000586 DASUCJ010000000
VERSION     DASUCJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831458"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /gene="radA"
                     /locus_tag="VFU90_06965"
     CDS             complement(<1..1264)
                     /gene="radA"
                     /locus_tag="VFU90_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5479557.1"
                     /translation="MSKARSVYACSGCGFESPRWLGRCPGCNAWNSFDERPFTVKPVR
                     ARAVPRGPAAVEPVALRDVDGERLVRMTLGMPEFDAALGGGIVPGSLTLVGGPPGAGK
                     STLLLQIAQRLARYGDVVYVCGEESAAQVKLRAQRLKIDAPVLVYPETNLRAVLDRLE
                     SAVPAVLVIDSIQTVWLPESEAYAGSVTQIRDCTQALIEFSKRTGCATLIVGHVTKDG
                     AIAGPRLLEHLVDTVLYFEGEANGEYRILRAYKNRFGSIDEICVFAMHDDGLAEVGNP
                     SELFLGSRAERPSGSCVVASVVGSRPLLIEMQALVGETSYGTPRRLANNLDQQRLAMV
                     LAVLERRAGLHLGTHDVYASVAGGLRVVEPAADLGIALAVVSSFRNLPLPPATAVFGE
                     LGLAGEIRQVAGADRRAAEARKLGYGELI"
BASE COUNT          241 a          476 c          496 g          209 t
ORIGIN      
        1 agatcagctc gccgtagccg agcttgcggg cctcggcggc gcgccggtcg gcgcccgcaa
       61 cctgccggat ttcgccggcc aaacccaact cgccgaagac cgcggtcgcg ggcggcagcg
      121 gaaggttgcg aaacgacgag acgaccgcca gggcgatgcc gagatcggcg gccggctcga
      181 cgacgcgcaa accgccggcc accgaggcgt agacgtcgtg ggtgccgaga tgcaagccgg
      241 cgcggcgttc gagaacggcg agcaccatcg cgaggcgctg ctgatcgagg ttgttggcca
      301 gccgccgcgg cgttccgtag ctcgtttcgc cgaccagcgc ctgcatttcg atcagcagcg
      361 ggcgcgagcc gacgaccgac gcgacgacgc acgatccgct cggacgctcc gcacgcgagc
      421 cgagaaacag ctccgatgga ttgccgactt cggcgagccc gtcgtcgtgc atcgcgaaga
      481 cgcagatttc gtcgatcgaa ccgaagcggt tcttataggc gcgcaggatg cggtattcgc
      541 cgttggcttc gccttcgaag tacaacacgg tatcgacgag atgctcgagc aggcgcggac
      601 cggcgatcgc gccgtctttg gtgacgtggc cgacgatcag cgtggcgcag ccggtgcgct
      661 tggagaattc gatcaacgcc tgggtacagt cgcgaatctg ggtgacgctg ccggcgtagg
      721 cttcggattc gggcaaccaa acggtttgga tcgaatcgat caccaggacc gcgggcacgg
      781 cgctctcgag ccggtcgagg acggcgcgca aattcgtttc cggatagacg agcacgggcg
      841 cgtcgatctt caagcgctgc gcgcgcagct tcacctgggc cgcggattct tcgccgcaca
      901 cgtagacgac gtcgccgtag cgcgccagcc gctgggcgat ttgcagcagc agcgtcgatt
      961 tcccggcgcc gggcgggccg ccgaccaggg tcaggcttcc gggcacgatg ccgccgccga
     1021 gcgccgcatc gaactccggc atgccgagcg tcatccgcac gagccgttcg ccgtcgacgt
     1081 cgcgcagcgc caccggttcg acggcggccg gaccgcgcgg caccgcgcgg gcgcgtaccg
     1141 gttttaccgt aaaagggcgt tcgtcgaacg agttccacgc gttacagccc gggcaacgcc
     1201 cgagccaccg cggcgactcg aatccgcagc cggaacaggc gtagaccgat cgcgctttgc
     1261 tcacggtacg ggtttttcgg cggcggtttg cgaaaaccgc cgcccgtgag ccagacgact
     1321 ccgctgcaga tcgcgatcga cggaccggcc gcttcgggga aaacgacggt ggcgcggctg
     1381 ctcgcgcgcc ggctgaacgc gttgtatctc gacaccgggg cg
//
LOCUS       DASUCJ010000587         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_785540, whole genome shotgun sequence.
ACCESSION   DASUCJ010000587 DASUCJ010000000
VERSION     DASUCJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785540"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFU90_06970"
     CDS             complement(<1..551)
                     /locus_tag="VFU90_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479558.1"
                     /translation="MEDELSRYVTALYEMLSSVLGQDKLVLRAGKVNALRMMRSQNLP
                     DRLCALQRLVFEDPTLERATTRAQQRKTIAEIEEAMADAIAQRHAEEAIDRRVNEKMA
                     ERHEDYLKDLKLEALRETTGPETPASKAKLDELDALSQRSLAASALQQLRPQTLRDIV
                     GQEAAIKALLAKLTSPYPQHVIL"
     gene            complement(618..1067)
                     /gene="rplI"
                     /locus_tag="VFU90_06975"
     CDS             complement(618..1067)
                     /gene="rplI"
                     /locus_tag="VFU90_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5479559.1"
                     /translation="MKLILLADVKPLGKKGEVVDVAEGYARNYLLPRKLATEADKGAL
                     ARLGSQKKASEKREAQVLADAKALAARLEAAKVAVKAKAGGNGKLFGAVTNADVASAI
                     HDTLAVAIDKHKIELKSAIKALGSYSVEIKLHKNVVAKTTVDVVSTT"
     gene            complement(1074..>1275)
                     /gene="rpsR"
                     /locus_tag="VFU90_06980"
     CDS             complement(1074..>1275)
                     /gene="rpsR"
                     /locus_tag="VFU90_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5479560.1"
                     /translation="RKSCNFCGDKVLDISYRDVNRLKKYVSERGKIVPRRISGNCAKH
                     QRLLTVAIKRARIVAFLPYVSE"
BASE COUNT          194 a          395 c          412 g          274 t
ORIGIN      
        1 tacaagatga cgtgctgcgg gtacggcgac gtcaacttcg cgagcagcgc tttgatggcg
       61 gcttcctgcc cgacgatgtc gcgcagcgtt tgcggccgca gctgctgcag cgccgaagcc
      121 gccagcgatc gctgcgagag cgcgtcgagc tcgtcgagct tggccttcga tgcgggcgtc
      181 tccgggccgg tcgtctcgcg cagcgcttcg agcttgagat ccttgagata gtcttcgtgc
      241 cgctcggcca tcttctcgtt gacgcggcgg tcgatcgctt cttcggcgtg gcgctgcgcg
      301 atcgcgtcgg ccatcgcttc ctcgatctcg gcgatcgtct tgcgctgctg ggcgcgggtg
      361 gtcgcgcgct ccagcgtcgg gtcttcgaac accaagcgct gcagcgcgca cagccggtca
      421 gggagatttt gcgaccgcat catgcgcagc gcgttgacct tgccggcgcg cagcacgagc
      481 ttgtcttggc cgagcaccga agacagcatt tcgtagagcg cggtcacgta ccggctgagt
      541 tcgtcttcca cgccgaactt tcgccgaaag cgcgacgcgt aggaaggttg cgcgctcccc
      601 gccacgacga cgtccgccta cgtggtcgaa acgacgtcga cggtggtttt tgcgaccacg
      661 tttttgtgga gcttgatttc caccgagtag gagcccagcg ctttgatcgc gctcttcagc
      721 tcgatcttgt gcttgtcgat ggcgacggcc agcgtgtcgt gaattgcgga cgcgacgtcg
      781 gcgttggtca ccgcgccgaa cagcttgccg ttgccgccgg cctttgcctt gaccgcgacc
      841 ttggcggctt ccagccgcgc ggccagcgct ttagcgtcgg ccaagacttg ggcctcgcgt
      901 ttttccgacg cttttttttg cgagccgagc cgggcgaggg cgcccttgtc ggcttcggtg
      961 gcgagcttgc gcgggagcag atagttgcgc gcgtatccct cggcaacgtc caccacctcg
     1021 cccttcttac cgagcggttt cacgtcggct agaagaatga gcttcacgat tgattactcc
     1081 gaaacgtaag gaaggaaggc gacgatccgt gcgcgtttga tcgcgacggt gagaagacgc
     1141 tgatgcttgg cgcagttgcc ggagatgcgg cgcggaacga tcttgccgcg ctcggaaacg
     1201 tacttcttca aacggttcac gtcgcggtag ctgatgtcca gaaccttatc gccgcagaaa
     1261 ttacatgact tgcgc
//
LOCUS       DASUCJ010000588         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_908051, whole genome shotgun sequence.
ACCESSION   DASUCJ010000588 DASUCJ010000000
VERSION     DASUCJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908051"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFU90_06985"
     CDS             complement(<1..946)
                     /locus_tag="VFU90_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5479561.1"
                     /translation="MKNIPYALGHRVLVVDDNPNLQKIISTNLELAGYEVATVSDGRE
                     ALATIEHDVPDLLLLDLMMPNMDGYEVARRVRSHANPSVANVPIIMLTAKGETEDKLR
                     GFEAGADDYITKPFGSRELLARVRAKIRRVEVDTALSPLTRLPGNLAIEGDIRRRLAA
                     GELFAVLYLDLDNFKAFNDVYGFTHGDEAIQLVARAAVDAVRRRGTQDDFIGHIGGDD
                     FIIVTQPDRAEEIAREILDDFDRSIRALYSARDLRLGYIVTRDRRGAVNRFPIMSLSI
                     AIVANQTRKLEDYAQVGEIAAELKQHAKSIAGSVYVKDK"
     gene            complement(956..>1219)
                     /locus_tag="VFU90_06990"
     CDS             complement(956..>1219)
                     /locus_tag="VFU90_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479562.1"
                     /translation="VGATNLYIRIPFICEGLLDGLAGALLAVGVLAIARATLWPRLLE
                     ALPWIQLNAVAINPVMLVAQLLAVGGAVGIVASWISVGRHLRT"
BASE COUNT          217 a          383 c          406 g          213 t
ORIGIN      
        1 gtttatcctt tacgtacacc gatccggcga tcgacttggc gtgctgcttc agctcggcgg
       61 cgatctcgcc cacctgggcg tagtcttcca gtttgcgcgt ttggttggcg acgatcgcga
      121 tcgaaagcga catgatcgga aaacggttga cggcgccgcg ccggtcgcgc gtgacgatgt
      181 agcccaagcg caggtcgcgt gccgagtaca gcgcgcggat gctccggtcg aaatcgtcca
      241 aaatctcgcg cgcgatttcc tcggcgcgat ccggctgcgt gacgatgatg aaatcgtcgc
      301 cgccgatgtg gccgatgaaa tcgtcttgcg tcccgcggcg gcgcaccgcg tcgaccgccg
      361 cgcgagcgac gagctggatg gcttcgtcgc cgtgcgtgaa tccgtagacg tcgttgaacg
      421 ccttgaagtt gtcgagatcg agatacagca ccgcgaacag ctcgcccgca gcgagccggc
      481 gccggatatc gccctcgatg gcgaggttgc cggggaggcg agtcagcggc gatagcgccg
      541 tgtcgacctc gacgcgccgg attttcgcgc gcacgcgcgc cagcagctcg cgcgagccga
      601 acggcttggt gatgtagtcg tcggccccgg cctcgaaacc gcgaagcttg tcttcggtct
      661 cgcccttggc ggtcagcatg atgatcggaa cgttggcgac cgaggggtta gcgtggctgc
      721 gcacccgccg cgcgacttcg tacccgtcca tattcggcat catgagatcg agcagcaaga
      781 gatcgggaac gtcgtgttcg atcgtcgcga gcgcctcgcg cccgtcggag acggtcgcca
      841 cctcgtagcc ggccagctcg agattggtcg agatgatctt ttgcaggttc gggttgtcgt
      901 cgacgaccaa gacgcggtgg ccgagggcgt acggaatgtt cttcacgtgc gccggttacg
      961 tccgtagatg gcgtccgacc gaaatccacg acgcgacgat gccgacggcg ccgccgaccg
     1021 cgagcagctg agcgaccagc atcaccggat tgatcgcaac ggcgttgagc tgaatccacg
     1081 gcagggcttc gagcagcctc ggccagagcg tcgcgcgcgc gatcgccaaa acgccgaccg
     1141 ccagcaaagc cccggccaga ccgtcgagca aaccttcgca gatgaacgga atgcgaatgt
     1201 agagattggt cgcgccgac
//
LOCUS       DASUCJ010000589         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1244514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000589 DASUCJ010000000
VERSION     DASUCJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFU90_06995"
     CDS             complement(<1..630)
                     /locus_tag="VFU90_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5479563.1"
                     /translation="MQGVVRIAHDDLERRMSQPNPAYNYAGEEFLIYELSQLLNVPAT
                     RVVDETQLPLLMTRTGDQRAAVRIDSVIGSREIVVKSVGPQISSIPGIFGATIMGDGS
                     VVMILDLAPLVRRSAALRERAEAAGEVAILPVAPAAPVESVIEQRAHPLIMVVDDSIT
                     MRKVTTRVLERNDMEVVTAKDGLDAVEKLQDKVPDIMLLDVEMPRMDGYE"
     gene            604..>1003
                     /locus_tag="VFU90_07000"
     CDS             604..>1003
                     /locus_tag="VFU90_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479564.1"
                     /translation="MGDAHHALHRRHRHCVRRFAEAHQNRLRHRQRERQADDERAART
                     ARGIDGERTAELLDLVVHDVHADAAAGKLGHLLSGREAGFQDEAVQVAVGQLDVGFEQ
                     AAFDRLAPDRVAIHAAAVVGHAQHDFRRLAA"
BASE COUNT          162 a          334 c          329 g          178 t
ORIGIN      
        1 ctcgtagccg tccatgcgcg gcatttccac gtcgagcagc atgatgtccg gtaccttgtc
       61 ctgcagtttc tcgaccgcgt ccaggccgtc cttcgcggtg accacttcca tgtcgttgcg
      121 ttcgagcacg cgcgtcgtga ccttgcgcat ggtgatcgaa tcgtcgacca ccatgatcaa
      181 tggatgcgcg cgctgctcga tgacggactc gaccggggcc gcgggcgcca ccggcaggat
      241 tgcgacttcg ccggctgcct ccgcgcgctc gcgcaacgcg gcgctgcggc gcacgagcgg
      301 agcaagatcg agaatcatca cgaccgagcc gtcgcccatg atcgtcgcgc cgaagatgcc
      361 ggggatcgaa ctgatctgcg gaccgaccga cttgaccacg atttcgcgcg aaccgatcac
      421 gctgtcgatg cgtacggcgg cgcgctgatc gccggtgcgg gtcatgagca gcggcaactg
      481 cgtctcgtcg accacgcgcg ttgccggcac attgagcagt tgcgacaatt cgtagatcag
      541 gaattcctcg ccggcatagt tgtaggccgg gttgggctgg ctcatgcgcc gctccaggtc
      601 gtcgtgggcg atgcgcacca cgccctgcac cgaagacatc ggcactgcgt acgtcgcttc
      661 gccgaggcgc accaaaatcg cctgcgtcac cgccagcgtg aacggcaggc ggatgatgaa
      721 cgtgctgccc gcaccgcgcg tggaatcgat ggcgagcgta ccgccgagct gcttgatctc
      781 gttgtgcacg acgtccatgc cgacgccgcg gccggcaagc tgggtcacct gctcagcggt
      841 agagaagccg gtttccagga tgaagccgta caggtcgcgg tcggacagct ggacgtcggg
      901 tttgagcagg ccgcgttcga tcgccttgcg ccggatcgcg tcgcgatcca tgccgcggcc
      961 gtcgtcggcc acgcgcagca cgacttccgt cgcctcgcgg ctg
//
LOCUS       DASUCJ010000590         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_556524, whole genome shotgun sequence.
ACCESSION   DASUCJ010000590 DASUCJ010000000
VERSION     DASUCJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556524"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFU90_07005"
     CDS             <1..639
                     /locus_tag="VFU90_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5479565.1"
                     /translation="DVVNVSLGGYLRRSSPFDLAVADLIQRAIDYATTRGALVVAAAG
                     NEALNSNTATGPHGSYADSVHVLAGGIRQVISVGATGPVNGTNFDSIAVYSNFGKVDV
                     AVFAPGGGLVTQDSANAADLVIGACSAALFSVCADERHYVANAGTSFSSPLVAAEAAV
                     IKAQAVTKPTPSALESCILSSADAPKGKSKPDINYNFGRIDVLNGVLHSKCK"
     gene            complement(683..1195)
                     /locus_tag="VFU90_07010"
     CDS             complement(683..1195)
                     /locus_tag="VFU90_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5479566.1"
                     /translation="MAAAMVPGLVAAQSSSSPVADAFRDNASRVGKNLIAAAETMPAD
                     KYSFKPTPAQMSFGQIVVHLSQGNDYLCGAIGGEKAPTRTKIADTASKEELVARLKET
                     FAFCDQALAKLDDSKLSEQLPFFGGKTRSRAAIMTLTTGDWADHYSQSSVYLRMNGEL
                     PPTAKGKQAD"
BASE COUNT          224 a          430 c          399 g          235 t
ORIGIN      
        1 gacgtcgtca acgtcagcct cggcggctac ctcagacgct cgagtccgtt cgatctcgcc
       61 gtcgcggatc tcatccagcg cgccatcgat tacgccacga cgcgcggtgc gttagtcgtc
      121 gccgccgcgg gcaacgaagc cctcaatagc aacaccgcta ccggcccgca cggcagctac
      181 gccgatagcg tgcacgtgct cgccggtggt atccgccagg tgatcagcgt gggcgcgacg
      241 ggaccggtca acgggaccaa cttcgactcg atcgccgtgt actcgaattt cggcaaggtc
      301 gatgtcgcgg tgttcgcgcc gggcggcggc ctcgtcacac aagattccgc gaatgctgcc
      361 gatctggtga tcggcgcctg cagtgcggcg ctgttctcgg tgtgcgcgga cgagcgccac
      421 tacgtcgcga acgcgggcac gagcttctcg tctccgctcg tcgccgcgga agcggcagtg
      481 atcaaggcgc aggcggtcac caaaccgacg ccaagcgcgc tcgagtcctg catcttgagc
      541 tcggccgacg cgccgaaggg caagtcgaag ccggacatca actacaactt cggacggatc
      601 gacgtgctca acggagtgct ccactccaag tgcaagtaaa acatccggag ctccgccgac
      661 ccgtccgccc aactcgatag cgtcagtccg cctgcttgcc cttcgccgtc ggcggcagct
      721 cgccgttcat ccgcaaatac acggaagact ggctgtaatg gtcggcccag tcacccgtgg
      781 tcagcgtcat gatcgcggcg cgcgaccgtg ttttcccgcc aaagaacggt agctgctcgc
      841 tcagcttcga gtcgtccagc ttcgccagcg cctggtcgca gaacgcgaac gtttccttga
      901 ggcgcgcaac gagctcttcc ttactggcgg tatccgcaat cttggtgcgc gtgggcgcct
      961 tctcgccgcc gatcgcgccg cacaggtagt cgttgccctg cgacaggtgc acgacgatct
     1021 ggccaaagct catctgcgcg ggcgtcggct tgaagctgta cttatcggct ggcatcgtct
     1081 cggctgccgc gatcaagttc ttaccgacac ggctggcatt gtcgcggaac gcgtctgcca
     1141 ccgggctcga cgacgattgc gccgccacaa gtcccggcac catggcggcg gccatggtcg
     1201 caatacaaat acggacacgc atggatctcg ctcgggttaa ggtaggcgtt gagtttggtc
     1261 cacgttgtgg gcgatgtcca gaccaggg
//
LOCUS       DASUCJ010000591         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1183061, whole genome shotgun sequence.
ACCESSION   DASUCJ010000591 DASUCJ010000000
VERSION     DASUCJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183061"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFU90_07015"
     CDS             complement(<1..370)
                     /locus_tag="VFU90_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479567.1"
                     /translation="MAPASPSAALSVLALGFLLGLRHATDADHVLAVATIVSRERRPL
                     RSSWVGVLWGIGHTLTILLVGGAILFFRLVVPPRIGLCLELAVAAMLVTLGTANLVRP
                     PGHVAAATEGAATHHGPHDRT"
     gene            complement(414..779)
                     /locus_tag="VFU90_07020"
     CDS             complement(414..779)
                     /locus_tag="VFU90_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479568.1"
                     /translation="MPTLIPKPTRIVAAGNKEKIIEEYVGRVNSKTSAASVARMKSPS
                     GWIEPGQSPEFDEYTLVLHGTLRVTSKSGVLDVHAGQAIIAHAGEWVQYSSPGDEGAE
                     YVAICLPAFSIDTVHRDPK"
     gene            complement(797..>1098)
                     /locus_tag="VFU90_07025"
     CDS             complement(797..>1098)
                     /locus_tag="VFU90_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479569.1"
                     /translation="PAASILGPSFGGTTAKQPFVVACASGAPAVGIYGTADHLVRSLG
                     LVCAHGGETQKTSTVGERRANGAAGFDLRCPPSTSLVGIEGRAGDLVDAVGIACR"
BASE COUNT          185 a          381 c          358 g          174 t
ORIGIN      
        1 gcgtccgatc gtgggggcca tgatgcgtag ccgcgccttc cgtcgccgct gcgacatgcc
       61 ccggaggccg caccaggttg gccgtgccca acgtcacgag catggcggcc accgcaagct
      121 ccaagcacag accgattcgc ggcgggacga ccagccggaa gaacaagatc gcaccgccga
      181 cgagcagaat ggtcagcgtg tgtccgatcc cccatagaac gccgacccac gaggaccgca
      241 gcggccgccg ttcgcggctc acgatcgttg ctaccgcgag gacgtggtcg gcgtccgtcg
      301 catggcgaag cccgagaagg aacccgagcg ccagaacgga gagggcggcg ctcggggacg
      361 cgggagccat gcccagcgac gtagcacgac gcttcgtagg tgtgccacga agtctatttc
      421 gggtcgcgat gcaccgtatc gatcgagaag gccggtaggc agatcgccac gtactctgcg
      481 ccttcgtccc ccggcgacga gtactgcacc cattcgccgg cgtgcgcgat gatcgcttgg
      541 cccgcgtgca cgtcgaggac gccggatttc gacgtgacac gcaacgtccc gtggagcacg
      601 agcgtgtact catcgaactc gggcgactgc ccgggctcga tccatccgct cgggctcttc
      661 atgcgcgcca cactggccgc gctcgtcttc gaattgacgc gcccaacgta ctcttcgatg
      721 attttctctt tgttgccggc ggcaacgata cgcgtgggct tgggtatgag ggtcggcatc
      781 ccgttaccgt aacccgttac cggcaggcga tccccaccgc gtccacgagg tcaccggcgc
      841 gcccttcgat cccgacgagg gacgtcgagg gcgggcatcg cagatcgaag ccggccgcac
      901 cgttcgccct tcgctcgccg accgtgctcg tcttttgcgt ctctccgcca tgcgcacata
      961 cgaggccgag cgatcggacc agatggtccg ccgtgccgta gatcccgacc gcgggtgcgc
     1021 cgctcgcgca cgccactacg aagggctgct tcgcggtggt tccgccgaac gatgggccca
     1081 ggatcgaggc cgccggag
//
LOCUS       DASUCJ010000592         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_510766, whole genome shotgun sequence.
ACCESSION   DASUCJ010000592 DASUCJ010000000
VERSION     DASUCJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510766"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFU90_07030"
     CDS             <1..340
                     /locus_tag="VFU90_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU5479570.1"
                     /translation="CDGGACDLVVTTGGTGLSKRDVTPEATRAVISAEAPGFGERLRD
                     HATRFPRASLSRGVAGVRARTLIVNLPGSPGGVRDGLATLEPVVSHACDILSGRFTEH
                     PAEHRSRDGA"
     gene            402..>1107
                     /locus_tag="VFU90_07035"
     CDS             402..>1107
                     /locus_tag="VFU90_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HEU5479571.1"
                     /translation="MLEAAGITTSMVSAVDNLATEIRRTRPELIVLTGNIADPAVRQV
                     VRDQLWEGIAVIGLADVADPQLIERLRAIGFVDVYTKPVVAEDLFDAVKRVLERRRLA
                     ESTGLVGQSDAIREVLVRVEQMAPVSSTVLIEGESGTGKELVARAIHQLSPRRSRPFI
                     AVNVGALPETLLESELFGHEKGAFTGAAERRIGRFELADTGTLFLDEIGDVPPATQVK
                     LLRVIEEREVTRVGGTT"
BASE COUNT          172 a          335 c          399 g          201 t
ORIGIN      
        1 ctgcgacggc ggcgcatgcg atctcgtcgt cacgaccggc ggcaccgggt tgtcgaagcg
       61 cgacgtcact cctgaagcaa cacgcgccgt gatatcggcg gaggcgcccg gcttcggcga
      121 gcggctgagg gatcacgcga cgcgttttcc gcgtgcgtca ctttcgcgcg gtgtcgccgg
      181 cgtgcgcgca cgaacgttga tcgtgaacct tcccggctca ccgggtggcg tgcgcgacgg
      241 gctggctacg ctggagccgg tggtgtcgca tgcgtgcgac attctgagtg ggcgttttac
      301 cgagcatcca gccgagcatc ggagccgtga tggcgcgtag cggcggtgca accgtggtga
      361 tcacgctcgc cgacgtggag cccgcggtgc gtctcaacgc gttgctcgaa gccgccggca
      421 tcaccacgtc gatggtttcg gctgtggaca atctcgcgac cgagatccgt cgtacgcgac
      481 cggagctgat cgtgctgacg ggaaacatcg ccgaccctgc ggtgcgccag gtcgtgcgcg
      541 accagctctg ggaaggaatt gcggtgatcg gccttgccga cgtcgccgac ccgcagctga
      601 tcgagcgctt gcgtgcgatc ggttttgtgg atgtgtacac caagcccgta gtcgcggaag
      661 atctgttcga cgcagtgaag cgtgtgctcg agcgtcgccg gctcgctgaa tcgaccggac
      721 ttgtgggcca gtcggacgcg atccgtgaag ttctcgtgcg cgtggagcag atggcgcccg
      781 tgtccagcac ggttctgatc gagggcgaga gcgggaccgg caaggagctt gttgcgcgtg
      841 caattcatca gttgagtcca cgccggtcgc ggccgttcat cgctgtcaac gtcggtgcgc
      901 tgccggaaac gttgctcgag agcgagctgt tcggtcacga gaagggtgcg ttcaccggcg
      961 ctgccgagcg ccgcatcggc cgcttcgagc ttgccgatac cggaacgctg ttcctcgacg
     1021 agatcggcga cgtgccgcca gcgacccagg tcaagctgct gcgcgtgatc gaggagcgtg
     1081 aggtgacgcg ggtcggagga acgacgc
//
LOCUS       DASUCJ010000593         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_801392, whole genome shotgun sequence.
ACCESSION   DASUCJ010000593 DASUCJ010000000
VERSION     DASUCJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801392"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFU90_07040"
     CDS             <1..691
                     /locus_tag="VFU90_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5479572.1"
                     /translation="YAAITERNVVAQQICSIEDPIEIRIPGTAQMQVNVRAGLTFAVG
                     LRSFLRQDPNVIAVGEMRDPETAAVAASAALCGQLVLTTLHSGNALGAFERLRELGMT
                     SRRIGNAVTAIASQRLLRRLCAECRVPAAAGAEAVRFGVARGSPANVARGCEACAGSG
                     YRGRMGVFEIVAMSSELRHAIETEAAPGELRDAALRGGYLPMARAAGQLVTTGESSVE
                     EASRVLSAGLA"
     gene            688..1758
                     /locus_tag="VFU90_07045"
     CDS             688..1758
                     /locus_tag="VFU90_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5479573.1"
                     /translation="MNAALLPAVRFFDALEAARERGGSDVHVSAGLPPVLRVDGSLER
                     LPEEPFSAEDVCAIVTELFRDDQFARLKAKGDHTTARCDARFGTLRVHAYAGMGGWTI
                     AVRLLDREIPSLEALRLPQAMHSLAGRERGLVIIAGPTGSGKSTTLAALVDRINDTSG
                     RRIVTIEDPIEYRHESRQSVVTQRELGRDTPELGVALRGALRADPDVIVVGEMRDAES
                     MAGALTAAETGHLVMTTLHTGNAAQTVERIVDSFSGSQQTQVRTQLAQVLAAVACQRL
                     VPRARGIGRCAIVELLFGTDAVRNLIRESKAHQLKNVMATGKRFGMQTFEQHVAELVR
                     DRELDPAEAVRLGFADAGKTVA"
     gene            1760..2974
                     /locus_tag="VFU90_07050"
     CDS             1760..2974
                     /locus_tag="VFU90_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5479574.1"
                     /translation="MLLYYAGRTIEGTRVRGSIEAATRETALGHLRSRAVFVTTLETA
                     ATVRGAWTRLGIMLGRDAVPPAAFYRSFGTLIAAGIPVRRALETLIRQTDGGFAEVLS
                     SIAADVENGSALSKAMELHPHEFSRIAIAIVRAGETGGTLDEALQVLAELEERDRGLR
                     KRLSAALAYPIAVVVAASGLVAFLLVNTMPSFAAMFSAMHVALPLPTRVLIAIGVRLQ
                     DPRSWIAAAAFAASIAVAARRLKESDSPLAALADRARLRSPVLGGVIAKTAAARFART
                     LGSLLRAGVDVVLALDAAAGVVDGFVYRSGLTAVDRALRRGETLTSAFAASGLFDATF
                     LQLVRAGEESGGLDPMLLRLAHYYELDVETALATLTTVLEPALICILGIVIGTIVASI
                     IIPLYTMIGNIQ"
     gene            2971..3687
                     /locus_tag="VFU90_07055"
     CDS             2971..3687
                     /locus_tag="VFU90_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5479575.1"
                     /translation="MTQLPSNLELWTRDGRREHRDAVVAEYRYLCRRAARRFVRAGLD
                     PADLEQVAAIGLIKAVDRYDPKQTTPFEAYAWLLVLGELMHHVRDGERLLRAPRRVRD
                     LERRWAVAERDLWRVLGREPREDDVATYVNATPAERREVRDYRASCRLVSFEVVRVSE
                     RRQGAAAYDAVLDKMTLEKFFAALSPLESQIVRSIHLEGSSVVDVAKKLGYSRRHLTR
                     LHRNALLRLRDLGRNVSSPT"
BASE COUNT          665 a         1204 c         1338 g          688 t
ORIGIN      
        1 gtacgctgcg attaccgagc gaaacgtcgt cgctcaacaa atctgcagca tcgaggatcc
       61 gatcgaaata cggattccgg gaaccgctca aatgcaggtt aacgttcgag ccgggttgac
      121 gtttgcggtc ggccttcgtt cgttccttcg ccaagatcca aacgtgatcg cggtcggcga
      181 gatgcgcgat cccgaaaccg cggcggtggc ggcgtcggcc gcgctgtgcg gacagctggt
      241 gctgaccacg ctgcacagcg gcaacgcact cggtgcgttc gagcggctgc gcgagctggg
      301 gatgacgtcg cgacgaatcg gcaacgccgt aacggcgatt gcgtcgcaac gtttgctgcg
      361 gcgcttgtgt gccgagtgca gggtcccggc cgctgcgggg gcagaggcgg tgcgcttcgg
      421 agtagcccgc ggctcgcccg cgaacgtagc gcgcggctgc gaagcgtgtg cggggagcgg
      481 ctatcgcggc cggatgggag tttttgaaat agtggcgatg tcttcggagc tgcgccatgc
      541 gatcgaaacg gaggcagccc ccggcgagct acgggatgcc gcgcttcgtg gcggttacct
      601 tccgatggcg cgcgctgccg gacagctcgt gacgacgggt gaatcgagcg tcgaggaagc
      661 ctcgcgcgtg ttgagcgccg gactcgcatg aacgctgcgc tgcttccggc ggttcgtttt
      721 ttcgacgcgc ttgaagcggc acgcgagcgg ggcggctccg acgttcacgt atcggcgggt
      781 ttgccgccgg tcttgcgggt ggacggttcc ctcgaacggc tgcccgagga accgttctca
      841 gcggaagacg tgtgcgcgat cgtaacggag ctgtttcgtg acgaccagtt cgcgcgcctt
      901 aaggcgaagg gcgaccatac aacggcgcgg tgtgacgccc ggtttggaac gctgcgcgtg
      961 cacgcctatg cgggaatggg cggctggacg attgcagtcc gcctgctgga ccgagagatt
     1021 ccgtcgctgg aggcattacg gctacctcag gcgatgcatt cgttggcggg gcgcgagcgc
     1081 gggctcgtga tcatcgcagg ccccaccggc agcggtaagt cgacgacctt ggcggctctg
     1141 gtggaccgta taaacgacac gtcgggacga cgaatcgtga cgatcgagga tccgatcgaa
     1201 tatcgtcacg agagccggca gtcggtcgtc acccagcgag agctcggacg ggatacaccc
     1261 gaactggggg tagcgttgcg cggcgcactg cgcgccgatc ccgacgtgat cgtggtcggc
     1321 gagatgcgag acgccgagag catggcgggc gcgcttacgg cggccgaaac cggacacctc
     1381 gtcatgacga ccttgcacac cggaaatgcc gcgcagacgg tcgagcgaat cgtggattct
     1441 ttctcgggat cgcaacaaac gcaggtccgt acgcagctcg cacaagtgct cgccgccgtc
     1501 gcctgccaac ggctcgtgcc gcgggcccgc gggattggcc gttgcgcaat cgtggagctg
     1561 cttttcggaa ccgatgcggt gcgcaatctg attcgagaat cgaaggcaca tcagctcaag
     1621 aacgtgatgg ccaccggcaa gcgcttcggg atgcaaacgt tcgagcagca cgttgccgag
     1681 ttggtgcgcg accgcgagct cgatcccgcc gaggccgttc ggctgggatt tgccgacgcc
     1741 ggcaagacgg tcgcttaacg tgctgctcta ctacgcgggg cgcacgatcg aagggacgcg
     1801 agtgcgcggc agcatcgaag ccgcaacccg cgaaaccgcc ctcgggcact tgcgcagccg
     1861 ggcagttttc gtcacgacgc tggagacggc cgctacggta cggggagcgt ggacccgatt
     1921 ggggattatg ctcgggcgcg acgccgtgcc tccggccgcg ttttatcgtt cgttcgggac
     1981 gctcatcgcg gccgggattc cagtgcgccg ggctctcgag actttgatcc ggcagaccga
     2041 cggcgggttc gccgaagtgc tcagttcgat cgccgccgac gtcgaaaatg gatcggcgct
     2101 ctcgaaagcg atggaactcc acccccacga gttctcgcgc atcgcgatcg ccatcgttcg
     2161 tgcgggcgag acgggcggta cgctcgacga ggcgctgcaa gtgctcgcgg agttggagga
     2221 gcgcgatcgc ggcctccgta aacggcttag cgccgctctg gcgtatccga tcgcggtggt
     2281 cgtggctgcg tcgggtttgg tcgcgttctt gctcgtgaac acgatgccgt cgtttgccgc
     2341 gatgttctcg gccatgcacg tcgcgctgcc tctgccgacg cgcgtgttga tcgcgatcgg
     2401 agtgcgctta caggacccgc ggagctggat cgccgcggcg gccttcgcgg cgtcgatcgc
     2461 ggtcgccgca cggcgcttga aagagtccga ctcgccgttg gccgcccttg cagaccgcgc
     2521 gcgtttgcgc tcgccggtcc tgggaggagt catcgcaaaa acggccgccg cgcgatttgc
     2581 gaggacgctg ggatcgctct tgcgagccgg cgtggacgtc gtgctcgcgc tcgacgccgc
     2641 cgccggcgtg gtcgacggat tcgtctaccg cagcggactc accgcggtcg accgggcgct
     2701 gaggcgcggc gagaccctga cgtcggcttt cgccgcaagc ggcttgttcg acgcgacgtt
     2761 tctccagctg gttcgagcgg gtgaggagag cggcggcctc gacccgatgc tgctgcggct
     2821 ggcacactac tacgagctcg acgtcgagac ggcgctcgca acgctcacga ccgttctgga
     2881 accggccctc atctgtatcc tcggtatcgt catagggacg atcgttgcat ccatcatcat
     2941 cccactctac acaatgatcg gaaacataca atgacgcaac ttccttcgaa tctagaactc
     3001 tggacccgcg acggacggcg cgaacatcgc gacgcagtcg tcgccgaata ccggtatctc
     3061 tgccggcgcg ccgcccggcg attcgtacgc gccggtctgg acccggccga tctcgagcaa
     3121 gtcgccgcaa tcggcctgat caaagcggta gaccgttacg atcctaagca gacgacgccg
     3181 ttcgaagcct acgcgtggct gctggtactg ggcgagctca tgcatcacgt tcgcgacgga
     3241 gagcggttgc tgagggcccc gcgccgcgtg cgcgacctcg aacggcgctg ggccgtcgcc
     3301 gaacgggact tgtggcgagt gctcggacgg gagccgcgtg aggacgacgt cgccacgtac
     3361 gtcaacgcga ctccggccga gcgccgcgag gtgcgcgatt atcgcgccag ctgccggctg
     3421 gtttcgtttg aagtcgttcg cgtgtccgag cggcggcaag gcgccgcggc atacgacgcg
     3481 gtgctcgata aaatgacgtt ggaaaagttc tttgcggcat tatcgccgct ggaatcgcaa
     3541 atcgtacgct cgatccacct ggaaggcagc agcgtcgtgg acgtggcaaa aaagttaggc
     3601 tattcgcgac ggcacctgac tcggctgcac cggaacgcgc tattgcggct tcgcgacttg
     3661 ggaagaaacg tctccagccc gacctagccc gggcacttag ccgaacgaac gaaaggtctg
     3721 ccgccgatgg aacaacgtac gcgcttgcgc tcgtccgcag gacgggacgc gcggctcgag
     3781 atcgcgtccc agcgaggatt tacactgatc gaaatgatga tcgtcgtcgc gatcatcgcg
     3841 atcctcgtga gcattctcgt gccgaacttc gtcagagctc gggcgcaagc gcaaa
//
LOCUS       DASUCJ010000594         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_984446, whole genome shotgun sequence.
ACCESSION   DASUCJ010000594 DASUCJ010000000
VERSION     DASUCJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984446"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFU90_07060"
     CDS             <1..265
                     /locus_tag="VFU90_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479576.1"
                     /translation="GKNRMRPITMTTVAAILTLMPLALAIGHGSQMQQPLAIAIISGL
                     IVALPLVLLVMPVLYNVFTRGGRVRPAPPPAHPQSATDASSGS"
     gene            complement(343..741)
                     /locus_tag="VFU90_07065"
     CDS             complement(343..741)
                     /locus_tag="VFU90_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479577.1"
                     /translation="MPRSSKLKVIVPSVLCAALLAAPGFVPIAQAQAGAMSASTHVSP
                     LGDLSEYRTIAEDALKLTKSGDFAGAHKKMKDFETAWDNHEDSNKKKSYNTCRAIDNQ
                     LDFTLGKLEAKKPDQAACEKALENMISRLR"
     gene            complement(914..1681)
                     /locus_tag="VFU90_07070"
     CDS             complement(914..1681)
                     /locus_tag="VFU90_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5479578.1"
                     /translation="MMRWVRYRTSAGGVAFGTLDGESIDEHEGDPFAGARPTGSRANL
                     ADVALLAPCEPSKIVALWNNFHALAVKLDKPVPERPLFLIKPASSVAGPGDAIERPTG
                     YTGRIAYEGELGIVIGKRCRNVPPNEAADHIFGYTCVNDVTAVDVMTESPHFTQWCRA
                     KGYDTFGCLGPVIATGFDWATATVVTRVDGVERQSYPLSDMVFSPAEQVSLISHDMTL
                     VPGDVIACGTSLGVGSIRDGATVEVSIAGIGSLVNRL"
     gene            complement(1709..>2797)
                     /locus_tag="VFU90_07075"
     CDS             complement(1709..>2797)
                     /locus_tag="VFU90_07075"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5479579.1"
                     /translation="SMKIAIIGAGAIGGYVGVKLALAGEDVTFIVRGANLAAIRANGI
                     RLLAADGGEPLVARVKATDDYAAAGPQDMVILAMKAHQVEAVAPKVPQLFDRDTFVVT
                     MQNGIPYWYFHGHGGDLAGSPVRSVDPTGIIGDNIPPQRVLGCVVYPASELVAPGVIR
                     HIEGNRFPVGELDGSASERVQRTAECFTRAGFKAPVLPDIRAEIWLKLWGNLTFNPIS
                     ALTRSTLVDICQYGPTRSLAASMMSEAQAVAEKLGIRFRVGLDQRIAGAEKVGKHKTS
                     MLQDVEAGRAPEIDALVGSVLELGRLTGTPTPNIEAVHALVNLLARECAVCGDTRAQP
                     DAHTQPVRGAFAPPPAQALQSPSAVLQA"
BASE COUNT          454 a          923 c          947 g          473 t
ORIGIN      
        1 ggggaagaac cggatgcgcc cgatcacgat gacgacggtc gcggcgattc tcacgctgat
       61 gccgctggca ctggcgatcg gacacggctc gcagatgcag cagccgctgg cgatcgcgat
      121 catctccgga ttgatcgtcg cattgccgct cgtgctcctc gtgatgccgg tgctctacaa
      181 cgtcttcacc aggggcggtc gcgtccgccc ggcaccgccg ccggcgcacc cgcaatccgc
      241 gaccgacgcg agcagcggca gttgaacgaa tccgccccgg cgtgatgccg gggcgggacc
      301 tgcgaaccgg gaattgcagc gaagtggtgc gtcggtcttc gatcagcgca gccgcgagat
      361 catgttctcg agcgccttct cgcacgcggc ctggtcgggc ttcttcgcct ccagcttgcc
      421 gagcgtgaag tcgagctggt tgtcgatcgc gcggcaggtg ttgtagctct tcttcttgtt
      481 cgagtcctcg tggttgtccc aggcggtctc gaagtccttc atcttcttgt gcgcgccggc
      541 gaaatccccg gatttcgtca gcttcagcgc gtcctcggcg atcgtgcggt actcggagag
      601 atcgcccagc ggcgaaacgt gcgtcgacgc ggacatcgcg ccggcctgtg cctgcgcaat
      661 gggcacgaat ccgggtgccg cgagcagcgc ggcgcacaac acacttggaa cgatcacctt
      721 cagcttcgac gaacgtggca tggcagtctc cagggttggg cggaacggca gttccgcggg
      781 gtccggcatc gaacgaccga acgtttctct tgtggttgca gacgatcgtg gatcgcactg
      841 cgtgaagcac ggcgatcata gcagcgcacg tcgatgaaat cgagcgaaac gcggcacgct
      901 cgcggtgagc gagctagagg cgattgacga gcgagccgat gcccgcgatg ctgacttcca
      961 cggtcgcgcc gtcgcggatc gatccgacgc cgagcgaagt cccgcaggcg atgacgtccc
     1021 ccggaaccag ggtcatgtcg tgagagatca ggctgacctg ctcggccggc gagaacacca
     1081 tatcggacag cggatagctc tgccgctcca cgccgtcgac gcgcgtgacg accgtcgccg
     1141 tcgcccagtc gaaaccggtc gcgatcaccg gaccgaggca gccgaaggtg tcgtagccct
     1201 tggctcggca ccactgcgtg aaatgcgggc tctcggtcat cacgtcgacg gcggtgacgt
     1261 cgttgacgca ggtgtagccg aagatatggt cggcggcctc gttgggcggc acgttccggc
     1321 agcgcttccc gatgacgatg ccgagctctc cttcgtaggc gatgcggccg gtgtagcccg
     1381 tcggacgctc gatcgcgtct ccgggaccgg cgaccgagct cgccggcttg atgaggaaga
     1441 gcgggcgctc gggtaccggc ttgtcgagct tcaccgcgag cgcgtggaag ttgttccaca
     1501 gtgcgacgat cttcgacggc tcgcacggtg cgagcagcgc gacatcggcg aggttcgccc
     1561 tggaacccgt cggacgtgcc ccggcgaacg gatcgccctc gtgctcgtcg atcgattcgc
     1621 cgtcgagtgt gccgaatgcg acgcccccgg cgctcgtccg gtagcgcacc cagcgcatca
     1681 tcgcgaactc cggagcacgg cgtccgggtc aggcctgcag caccgcggac ggcgattgca
     1741 gcgcctgagc gggcggcgga gcgaacgcgc cgcgaaccgg ctgcgtgtgt gcgtccggtt
     1801 gtgcgcgtgt atcgccgcac accgcgcact cgcgcgcaag caggttgacg agcgcgtgca
     1861 cggcctcgat attgggcgtc ggcgtgccgg tcaggcggcc gagctcgagc accgagccga
     1921 ccagggcatc gatctcgggt gcgcgcccgg cctcgacgtc ctgcagcatc gacgtcttgt
     1981 gcttgccgac cttctcggcc ccggcgatgc gctgatcgag gccgacgcga aaacggatgc
     2041 cgagcttttc ggccacggcc tgcgcctcgg acatcatcga ggccgcgagc gagcgcgtgg
     2101 gcccgtactg gcagatgtcg acgagggtcg agcgcgtgag cgcgctgatc ggattgaacg
     2161 tcaggttgcc ccacaacttg agccagatct cggcgcggat gtcgggaagc acgggtgcct
     2221 tgaatccggc gcgcgtgaag cactcggcgg ttcgctgcac gcgctcgctc gccgacccgt
     2281 cgagctcgcc caccgggaaa cggttgccct cgatatgtcg gatcacgccc ggcgccacga
     2341 gctccgatgc cggatagacg acgcagccca gcactcgctg cggcggtatg ttgtcgccga
     2401 tgatcccggt cggatcgacg ctgcgaacag ggcttcccgc gagatcaccg ccgtggccgt
     2461 ggaaatacca gtacggaatc ccgttctgca tcgtgacgac gaacgtatcg cgatcgaaga
     2521 gttgcggcac tttcggtgcc accgcttcga cctggtgcgc cttcatcgcg aggatcacca
     2581 tgtcctgagg gcccgcggca gcgtaatcgt cggtcgcctt cacgcgcgcg acgagtggct
     2641 cgccgccatc ggcagcaagc aaacggatgc cgttcgcgcg gatggccgcg agattggcgc
     2701 cgcggacgat gaacgtgaca tcctcgccgg cgagcgcaag cttcaccccg acgtagcccc
     2761 cgatggcccc ggcacctatg atcgcgatct tcatcga
//
LOCUS       DASUCJ010000595         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_892994, whole genome shotgun sequence.
ACCESSION   DASUCJ010000595 DASUCJ010000000
VERSION     DASUCJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892994"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VFU90_07080"
     CDS             <1..1433
                     /locus_tag="VFU90_07080"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479580.1"
                     /translation="GGTLRLQMPVWSPGRYARMDFARNVRDESVQDANGHAVRFDREN
                     GSLWRIYPAGAGHVTVRYRVFADNLSGTFSVLDTAHANWNGASLFMYVVGHKPDSVSL
                     AVTPPAGWHIINGASTSPDQRHFRFANYDEMIDTPTEVAKYFDVDSFTVDNRLYRVVV
                     HHNGPQNGYHQGFVRAVERIVRYENTVIAPPPLAMYTFLFNVGYDGSDGMEHLYSTQI
                     QTRAPWTDSASVMATVEDAAHEYFHTWNVKRIRPLLLGPFDYSAERYQPSLWVAEGWT
                     QYYGEIGLLRAGIVSKGNYYQTLAHVLDANLESPGRKVVSARMASFLAPFWDGAATPM
                     RVDRSSFFSYYTKGEGLALVLDLDIRARTHNAKSLDDALRVLKRETWDAPNASYYLQG
                     RGYTEDDVVRAASEAAGADLAPWFDRYVAGVDDPPFAAALAQAGLRLESAPGPQGTEY
                     TVVEDPNATPDQLRVRHGWLTGTVGP"
     gene            complement(1497..>1698)
                     /locus_tag="VFU90_07085"
     CDS             complement(1497..>1698)
                     /locus_tag="VFU90_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479581.1"
                     /translation="VAVQQMVDVLGEQLGLPKEQAYALASVAADLKIAELVDGNVCVY
                     MTIPKKIAGRTFKPSAKNGAGT"
BASE COUNT          287 a          554 c          564 g          293 t
ORIGIN      
        1 taggcggcac cctccgcctc cagatgccgg tgtggtcgcc cggccgctac gcgcgcatgg
       61 acttcgcgcg caacgtgcgc gacgaatcgg tgcaggatgc gaacggccac gccgtacggt
      121 tcgatcgcga gaacggctcg ctgtggcgca tctatccagc gggcgcgggc cacgtcacgg
      181 tgcgctaccg cgtgttcgcc gacaacctct cgggcacgtt cagcgtcctc gatacggcac
      241 acgcgaactg gaacggcgcg tcgctcttca tgtacgtggt ggggcacaag ccggactcgg
      301 tgtcgctcgc ggtcacaccg ccagccggct ggcacatcat caatggcgcg agcacgtcgc
      361 ccgaccaacg ccatttccgg ttcgccaact acgacgagat gatcgacacg ccgaccgaag
      421 tggccaaata cttcgatgtc gacagcttca cggtggacaa tcggctctat cgggtagtgg
      481 tgcaccacaa cggaccgcag aacgggtatc accaggggtt cgtacgcgct gtcgagcgca
      541 tcgtgcgata cgagaacacg gtgatcgcgc cgccgccgct cgcgatgtac acgtttctgt
      601 tcaacgtcgg ctacgatggc agcgacggga tggagcacct ctactcgacc cagattcaaa
      661 cgcgtgcgcc gtggaccgac tcggcgtcgg tgatggcgac ggtcgaagat gcggcgcacg
      721 agtacttcca tacgtggaac gtgaagcgca tccgcccgtt gctccttggg ccgttcgact
      781 attccgcgga gcggtatcag ccgagtttgt gggtggccga gggttggacg cagtactatg
      841 gcgaaatcgg attgctgcgc gcgggcatcg tgagcaaggg caactattac cagaccctcg
      901 cgcacgtact cgacgcgaat ctggagtcgc ccggccgaaa agtcgtgtca gcacggatgg
      961 cgtcgttcct cgcgccgttc tgggatgggg cggcgacgcc gatgcgcgtg gatcgctcga
     1021 gcttcttctc gtactacacc aagggcgaag ggctcgcgct ggtactcgat ctcgacatcc
     1081 gggcgcggac gcacaacgcg aaatcgctgg acgacgcgct gcgggtgctc aagcgcgaga
     1141 cctgggacgc gcctaacgca agctactatc tgcagggccg cgggtacacc gaagacgacg
     1201 tcgtgcgcgc ggcgagtgag gcggccggcg ccgacctcgc gccgtggttc gaccggtacg
     1261 tggccggcgt ggatgacccg ccgttcgcgg ccgcgctcgc gcaggccggt ttgcggctgg
     1321 agtcagcgcc gggcccgcag ggcaccgagt acactgtggt cgaggatccg aacgcgacgc
     1381 ccgatcagct acgcgttagg catggctggc tcaccggtac cgtcggtccg tgagctcctg
     1441 cggctgatcg cacgtgtcgt gccccgtacg ctcggggtag atgccgcgtg cccgcgtcac
     1501 gttccagcgc cgttcttggc gctcggcttg aacgtccggc cggcgatctt cttcgggatc
     1561 gtcatgtaca cgcacacgtt gccgtccacg agctcggcga tcttgagatc ggcggcgacg
     1621 ctcgccagcg cgtacgcctg ctccttgggc aagcctaact gctcgcccaa cacgtcgacc
     1681 atctgctgca cggcgacg
//
LOCUS       DASUCJ010000596         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1381898, whole genome shotgun sequence.
ACCESSION   DASUCJ010000596 DASUCJ010000000
VERSION     DASUCJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381898"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFU90_07090"
     CDS             <1..283
                     /locus_tag="VFU90_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479582.1"
                     /translation="VLAIAATGAASLRLETSANRRAHAASSVSTRFELLHRAGCASSA
                     GADSALGVSAVWRSIGVDSVQQSVDSVVIVDRLAAHPITQVFKSAAPCR"
     gene            274..>1139
                     /locus_tag="VFU90_07095"
     CDS             274..>1139
                     /locus_tag="VFU90_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5479583.1"
                     /translation="MPMTRGSLRGFTLIELVIGLVVAAVVGATAISIAVRQEHLAFAL
                     EQIIETRRALREGVDALRYDLRATSPPRGGIYAMGDTYVEFRMATGFSILCGLDSSRT
                     LLVFPSRSSSVANLTSWISAPEAGDTVMVFTAHADADSARWDAYAMASATTSGRGCLA
                     DFRAPDATASTMAVRLTTPVPRGVGPGAALRFVRRARYELYKSADGGWYLGFRDCLAT
                     RATPCAIVQPVSGPYSVGGMRFVFRDSADAPTADPNRVTRIDVMMRAASAARLHLPGI
                     RPGVFVDSVLFT"
BASE COUNT          164 a          379 c          365 g          231 t
ORIGIN      
        1 cgtgctcgcc atcgccgcaa cgggtgctgc cagcctgcga ctcgaaacga gtgccaaccg
       61 gcgggcacac gctgcatctt ccgttagtac gcggttcgag ctgctgcatc gcgccggctg
      121 cgcgtcgtct gccggtgccg acagcgcact cggtgtttca gcggtctggc ggagcattgg
      181 cgtcgacagc gtccagcaaa gcgtcgacag cgtcgtgatt gtcgaccgac tcgccgcaca
      241 tcccatcacc caagtcttca aatcggcggc gccatgccga tgacccgcgg atcgctccgg
      301 ggcttcacgc tgatcgagct cgtcatcggc ctggtcgttg ccgccgtcgt cggagcgacg
      361 gcgatttcga tagctgtgcg ccaggaacat ctcgcgtttg cgctcgaaca gatcatcgaa
      421 acgcgtcgag cacttcgcga gggggtcgac gcgttgcgat acgaccttcg ggccacgtcg
      481 ccgccgcgtg gcgggatcta tgccatgggc gatacgtacg tcgagtttcg aatggcgacc
      541 gggttctcga ttctctgcgg gctcgactcc tcgcgcacgt tgctcgtgtt tccgtctcgc
      601 tcatcgtccg tagccaatct cacgtcgtgg atctcggcgc ccgaggcggg tgacaccgtc
      661 atggtgttca ctgcgcacgc cgatgctgat tccgctcgct gggatgcata cgccatggca
      721 tcggcgacga cgtcggggcg tggatgcttg gctgatttca gagcgccgga cgccaccgcg
      781 tcgacgatgg ccgttcgcct aacgaccccg gtgcctcggg gcgttgggcc tggtgccgcg
      841 ctgcgcttcg ttcgccgcgc caggtacgag ctgtacaagt ccgcggacgg cggctggtat
      901 ctgggctttc gcgactgtct cgctacgcgc gcgacgccct gcgccatcgt ccagcccgtg
      961 agtggcccgt attcggtggg cggcatgcgt tttgtctttc gagacagcgc ggacgcgcca
     1021 accgccgatc caaatcgcgt cacacgcatc gatgtcatga tgcgagccgc cagtgccgcg
     1081 cgtctgcatc tcccgggcat ccggccgggc gtattcgtcg attcagtgct attcaccat
//
LOCUS       DASUCJ010000597         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_572130, whole genome shotgun sequence.
ACCESSION   DASUCJ010000597 DASUCJ010000000
VERSION     DASUCJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572130"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFU90_07100"
     CDS             complement(<1..760)
                     /locus_tag="VFU90_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479584.1"
                     /translation="MKIKNARLALGACAMIGLLAGCAGGGASNGGVTGAVPQVRSVAM
                     RAPEALPQARILARLGRGLPAHSSSGRSWMDPSAVTSDLLYVSDTDTSEVYVFSYPQG
                     KPKGALTGFTDPAGVCVDAHGNVFVTNTGGFNVLEYAHGGTTPIATLKDAGYFPVGCS
                     VDPKTGNLAVTNFATSSSTQGNVVIYKGAKGRPTGFYTNPNMSEMLLCGYDASGNLFV
                     DGTTASSAAKFAELPSGKTKLVLLTLNQSIASPGG"
     gene            complement(833..1081)
                     /locus_tag="VFU90_07105"
     CDS             complement(833..1081)
                     /locus_tag="VFU90_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479585.1"
                     /translation="MLAYIGSSIWLLLLIGLVVGLLPIWFLWRIIDRTGLVGAMALLS
                     FLPGGFLVVLGVVAFSDWPNMPSAPERIPPQWTPGQPG"
     gene            complement(1110..2216)
                     /locus_tag="VFU90_07110"
     CDS             complement(1110..2216)
                     /locus_tag="VFU90_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479586.1"
                     /translation="MTDPNHGRPIEPQGHTLRAVRIILQDGKPRDAKQILAEAIARHL
                     LPPSTKEKYIYSALLEYIVRTSGHGHRPFVVQDSFRRFRLNEPVDEWPPEQPPPVPPP
                     DAATQALIDRLERTAPGGDPSAFELAVCDAFAHLGFVATHVGGRANPDGYADAALGVL
                     EYRTMLECKTGGTVVTNPDALEASKFKEQYGAQVCAIVGSRFSEKIELSTELQTHGVA
                     AFSVEDLQQLLRVRSNPVEMRPLFAPGFASDRIGDLLWERVHGLVKRVGLICDYVCEG
                     GWAAQMAYAKSGHPREASQAPRLTIDAAMLLVDQRLACEGTTATCARVEVEQAFADLT
                     SPRDGRAVWTSDARDAIVLTGASFDYGAARLRSG"
     gene            complement(2213..>2765)
                     /locus_tag="VFU90_07115"
     CDS             complement(2213..>2765)
                     /locus_tag="VFU90_07115"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479587.1"
                     /translation="QESAPSVPRFGRILLEPDREFHGSVAQAAIPMRDVLHREGLDAI
                     PLLDGRRGLALWVPIGLSMTYPELRRWMHAICNKTVEQHSDRFSVQPNTTVEGKVHLH
                     VDSNAPGRFSCLPYSLRGGEELYVCTPVRWEELPHVHDGDFTAETFPKRLAASGDLFD
                     GYLRTFGGDDDAVRVKPAGVRAP"
BASE COUNT          468 a         1013 c          816 g          468 t
ORIGIN      
        1 cgccgcccgg gctcgcgatg ctctgattga gcgtaagcag gacgagcttc gtcttcccgc
       61 tgggcagctc cgcaaacttc gccgcgctcg aggccgtcgt gccgtcgaca aacagattcc
      121 cgcttgcgtc gtacccgcac aacagcatct cactcatgtt cggattggtg taaaatcccg
      181 tcggacgccc cttcgcgcct ttgtagatca ccacgttgcc ttgcgtactg ctgctggtcg
      241 cgaagttcgt caccgccaga ttcccggtct tcgggtcgac cgaacacccc accggaaagt
      301 aaccggcgtc cttcagcgtc gcgatcggcg tcgtaccgcc gtgcgcgtac tcgagcacgt
      361 tgaacccgcc ggtgttcgtg acgaacacgt tgccgtgtgc gtcgacgcac acgccggccg
      421 gatcggtaaa tcccgtcagc gcgcccttcg gctttccttg cggatacgaa aacacgtaga
      481 cttcgctggt atcggtatcc gaaacgtaca gcaagtcgct ggtcaccgcg ctcggatcca
      541 tccacgaccg tccgctcgag gagtgcgccg gcagaccgcg tcccaaccgc gccaagatgc
      601 gcgcttgcgg caaagcctcg ggcgcgcgca tcgcaaccga tcgcacctgc ggtaccgcac
      661 cggttacgcc gccgttcgac gctccgccgc cggcacagcc cgccagcaat ccaatcatcg
      721 cgcacgcacc cagcgcaagc cgcgcgttct taatcttcat aaacgagcct gcctctttcg
      781 caaaacgaag cctcttgcat tgcggccgcc gccgccggct tcctcttgcg agctatcccg
      841 gctggccggg cgtccactgc ggcggaattc gctccggtgc gctcggcatg ttcggccaat
      901 ccgaaaacgc caccacgccg agcaccacga gaaaaccgcc cggcagaaac gacagcagcg
      961 ccatcgcccc tacgagtccg gtgcgatcga tgatccgcca cagaaaccaa atcggcagca
     1021 gacccaccac caacccgatg agcagcagca gccaaatcga ggatccgatg tatgccagca
     1081 tgcgcgctgg attcgccgcc tcccctgtgt catcctgagc ggaggcgcgc agcgccgtag
     1141 tcgaaggacg cacccgtcaa cacgatcgca tcgcgcgcgt cgctcgtcca caccgcgcgc
     1201 ccgtcgcgcg ggctcgtgag atcggcgaac gcctgctcca cttccacccg cgcgcacgtc
     1261 gccgtcgttc cctcgcacgc caatcgctga tcgaccagca gcatcgccgc gtcgatcgtc
     1321 agccgcggcg cttgtgacgc ctctcgcgga tgccccgact tcgcgtacgc catctgcgcc
     1381 gcccaaccgc cttcgcacac gtagtcgcag atcagcccga cgcgcttgac cagcccgtgc
     1441 acgcgttccc acaacagatc gccgatccga tcgctcgcaa accccggcgc gaacagcggc
     1501 cgcatctcga ccggattgct gcgcactcgc aagagctgct gcaggtcctc gacgctgaag
     1561 gccgccaccc cgtgtgtctg cagctcggtg ctcagctcga tcttctcgct gaaccgcgac
     1621 ccgacgatcg cgcacacctg cgccccgtac tgctccttga acttcgaagc ttccaacgcg
     1681 tcggggttcg tgacgacggt tccgcccgtc ttgcactcga gcatcgtccg gtactcgagc
     1741 acgcccagcg ccgcgtcggc atacccgtcg ggattggcgc gcccgccgac gtgcgtcgcg
     1801 acgaatccca gatgcgcgaa cgcatcgcat accgccagtt cgaatgcgct gggatcgccg
     1861 ccgggcgccg tgcgttccag ccggtcgatc aacgcctgcg tggccgcatc cggcggcggc
     1921 accggcggcg gctgttccgg cggccactcg tcgactggtt cgttgaggcg aaaccgccgg
     1981 aagctgtcct gcacgacgaa cggacgatgc ccgtgcccgc tcgtccgcac gatgtactcg
     2041 agcagcgcgg agtagatgta cttttccttc gtgctcggcg gcagcaagtg ccgcgcgatc
     2101 gcttcggcga gaatctgctt ggcgtcgcgc ggcttgccgt cttgcaggat gatccgaacc
     2161 gcgcgcagcg tgtgcccctg cggttcgatc ggcctgccgt ggttcgggtc ggtcacggcg
     2221 cccgaacgcc cgccggtttg acgcgcaccg catcgtcgtc gccgccgaac gttctgaggt
     2281 agccgtcgaa taggtcgccg ctggcggcca agcgtttggg aaacgtctcg gcggtgaagt
     2341 cgccgtcgtg cacgtgcggc aattcttccc accgcaccgg cgtgcagacg tacagctcct
     2401 cgccgccgcg caacgagtac ggcaaacagc tgaagcggcc gggcgcgttg gaatcgacgt
     2461 gcagatgcac tttcccctcg acggtggtgt tcggctgcac cgagaatcga tccgagtgct
     2521 gctcgactgt cttattacag atggcgtgca tccaacgccg cagctctggg tacgtcatcg
     2581 aaagcccgat cggaacccac agcgccaggc cgcgcctgcc gtcgagcaac ggaatcgcgt
     2641 cgagcccttc gcggtgcagc acgtcgcgca tcggaatcgc cgcctgcgcg accgagccgt
     2701 gaaactctcg atcgggctcg agcagaatcc gtccgaaccg cggcaccgac ggcgcgcttt
     2761 cctgg
//
LOCUS       DASUCJ010000598         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_862309, whole genome shotgun sequence.
ACCESSION   DASUCJ010000598 DASUCJ010000000
VERSION     DASUCJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862309"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFU90_07120"
     CDS             <1..384
                     /locus_tag="VFU90_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5479588.1"
                     /translation="ADELLADLMESRSTASAALDLTPTLTVVRNDRRDDATPAPEEAR
                     ITILTGPHAGEATPMNKAQFVFGRPGVSVAAIRRSGGAYRLVPLAGESPPVVNGGAVD
                     RTGADLAFGDTIEVAGVKLRFERRA"
     gene            652..1083
                     /locus_tag="VFU90_07125"
     CDS             652..1083
                     /locus_tag="VFU90_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU5479589.1"
                     /translation="MKRVQQGFTLIELMIVVAIVGILAAIALPAYQDYVIRSKMSEAE
                     AAIAACKTSVSEYTSTHAGTLPADTTASGCSTQSTHYVASLSVTSGVIVATSANTGAS
                     PGECVLSLTPTAVTDATIGTWTGTYQTGSCDSKYVPSSFRG"
     gene            1189..>1787
                     /locus_tag="VFU90_07130"
     CDS             1189..>1787
                     /locus_tag="VFU90_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479590.1"
                     /translation="MLGRLLRSEEKRVVSAADTVGSATSSTVHGDHGRPRAIALFAAG
                     RDWEASRAFERARNSGALDAECLRKLGWARFASGDAAGAEAAMREALVRAPYEWASHY
                     GVGAVLRGRDDHVAMTEFVAALELATGNVDCLLALSSCAQALGDASAAERYARDALAV
                     ASGRRDAWLALGVALLAQERLQEAHDAFARAEMLTDETFG"
BASE COUNT          311 a          567 c          603 g          306 t
ORIGIN      
        1 gcggacgagc tcctcgcgga cctgatggaa tcgcgctcga ccgcatccgc cgcgctcgac
       61 ctcacgccga cgctcaccgt cgttcgcaac gaccgccgcg acgacgctac gccggcgccg
      121 gaggaagctc gcattacgat actgaccggc ccgcacgccg gcgaagcgac gccgatgaac
      181 aaggcgcagt tcgtgttcgg ccgtccaggc gtgagcgtcg cggcgatccg ccggagcggc
      241 ggcgcctacc gcctcgtccc cctcgcggga gagtcgcccc cggtcgtcaa cggcggcgcg
      301 gtcgaccgca ccggagccga cctcgccttc ggcgacacga tcgaggttgc cggcgtgaag
      361 ctccggttcg aacgccgggc ctgatggggc gatgtgacgc tcagcgtcac atggatgcgg
      421 ccgtgctagg gcagcactta cacggctcgc cgatccgcca agccctggag ccggcccgag
      481 caactggctg acggcaggcg ccgaacgaga aaatcacttg ctgaacaata ggttgcgcgt
      541 ccggataggc ggcgacgcag gggtcaggtg tttggcatgt ggcttgctga actcgaccca
      601 gcatcgctgg atgccgatgt gaagcagtgc catcactcaa ggagaaattc aatgaaacgt
      661 gttcaacaag gcttcacgct catcgaactg atgatcgtgg tggcgattgt cggcatcctg
      721 gcagcgatcg ccttgccggc gtatcaggac tacgtgatcc gctcgaagat gtcggaagcc
      781 gaagccgcga tcgcggcgtg caagacgtcg gtgtccgagt acacgtcgac gcacgcgggg
      841 acgttgcctg cagataccac ggcgtcgggt tgcagcacgc agtcgaccca ctacgtggcg
      901 tcgctcagtg tgacgagtgg cgtgatcgtc gcgacgtcag cgaataccgg ggctagccca
      961 ggcgagtgcg tgctctcgct caccccgact gcggtcaccg acgcgacgat cggcacctgg
     1021 acgggcacct atcaaacggg ctcgtgtgac tcgaagtacg taccgtcctc gttccgcggc
     1081 tgattcctgt atccaggctg agcgctgcgc ggccacgatc gaaccagcac taccattcaa
     1141 ccggcgggag tgatcccgcc ggttttcatt tgtagatccg taataacgat gctcggtcga
     1201 ctgctcagga gtgaggagaa gcgcgtcgtg agcgccgcag acaccgtggg ctcggcgact
     1261 tcgagcaccg ttcatggtga tcatggccgg ccacgggcca tcgcactttt cgcggccggt
     1321 cgcgactggg aggcaagcag ggctttcgag cgagcgcgga acagcggtgc tctcgatgcc
     1381 gaatgcttgc gcaagctcgg ctgggcccgc ttcgcgagcg gtgacgcggc tggcgccgag
     1441 gcagcgatgc gcgaagcgct ggttcgggca ccttacgaat gggcatcgca ttacggggta
     1501 ggtgccgtcc tgcgcgggcg cgacgatcat gtcgcgatga ccgagtttgt cgcggcgctc
     1561 gaacttgcga ccggaaatgt cgattgcctc cttgctcttt cttcgtgtgc tcaggcacta
     1621 ggcgacgcct cagcggcgga gcgctacgca cgcgatgcgc tcgccgtggc atcggggcgt
     1681 cgcgacgcct ggttggctct cggcgttgca cttctcgcgc aggagcgcct gcaagaagcc
     1741 cacgatgcgt tcgcgcgggc ggaaatgttg accgacgaaa cgttcgg
//
LOCUS       DASUCJ010000599         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_419034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000599 DASUCJ010000000
VERSION     DASUCJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1179)
                     /locus_tag="VFU90_07135"
     CDS             complement(19..1179)
                     /locus_tag="VFU90_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479591.1"
                     /translation="MDRNIVRAIARLVAVSIVAAAAGCGAGNVGVEPAIGSVNPIATS
                     KLQFAVGTATINDVANNRVVEVLNTVETLRQPNGLSAVLYSIPYITGPADFEGQPDPQ
                     FGTPTNIISGSGPKIPCDKTTLQCTGGAFGYGFAPDNTVPNQQTVSFAHYIQPVAVGN
                     LDTLPILAYYGGEPAFPTFNNGTFPAGFQGYSAGFVSFQSPAVLGSYHLEVDIPTGPG
                     TFGKKTADATLTSLTGLPPMSAPSAFPDPQNPGGLKIDVTVPNGVTETWVFVQDSGGC
                     YPHGQGNSTNVQYYTVLSTQTGPQQLTVPPDLGPTNGSGTTPTICSSQDNQQASGNPN
                     APGDTYSVYAAGFDYPAFGAAYPFNLEQMPQFVGANGQVDVTLSPSASFVYP"
     gene            complement(1184..>1368)
                     /locus_tag="VFU90_07140"
     CDS             complement(1184..>1368)
                     /locus_tag="VFU90_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479592.1"
                     /translation="FNQIYGVPLVNLQYFEPVATGISGGANPNGYPGEAFPTSPFVIL
                     PDQPPLTMRFYWQIKV"
BASE COUNT          221 a          428 c          470 g          249 t
ORIGIN      
        1 gtcgaccgcc gcgtgccgct atgggtagac gaagctcgct gacggagaca gcgtgacgtc
       61 gacttgcccg ttcgcgccga cgaactgcgg catctgttcg agattgaacg gatacgcggc
      121 gccgaacgcc ggatagtcga acccggccgc gtagacggaa tacgtatccc ccggcgcgtt
      181 cggattgccg ctcgcctgtt ggttgtcttg cgacgagcag atcgtcggcg tcgtgcccga
      241 accgttcgtc gggccgagat ccggcggcac ggtcagctgc tgcggtcccg tctgcgtcga
      301 gagcaccgta tagtactgca cgttcgtcga gttgccctga ccgtgaggat agcagccgcc
      361 ggagtcttgg acgaagaccc acgtctcggt gacgccgttc ggcaccgtga cgtcgatctt
      421 gagcccgccc ggattctgcg gatcggggaa cgcggagggc gccgacatcg gcggcaatcc
      481 ggtgagcgac gtcagcgtcg cgtcggcggt cttcttgccg aacgttcccg gcccggtcgg
      541 gatgtcgacc tcgagatgat agctgccgag caccgccggg ctctggaacg agacgaagcc
      601 ggccgaatac ccttgaaagc ccgcggggaa cgtgccgttg ttgaacgtcg ggaacgccgg
      661 ctcgccgccg tagtacgcca agatcggcag cgtgtccaga tttccgacgg cgaccggttg
      721 tatgtaatgc gcgaacgaca ccgtctgctg gttcggcacg gtgttgtccg gcgcgaagcc
      781 gtagccgaac gcaccgcccg tgcactgcag cgtcgtcttg tcgcacggta tctttgggcc
      841 gcttcccgag atgatgttcg tgggggtgcc gaactgcggg tccggctggc cttcgaaatc
      901 cgccggcccc gtgatgtacg ggatgctgta gagcacggcc gacagcccgt tcggctgccg
      961 cagcgtctcg accgtgttca gcacttcgac cacccggttg ttcgcgacgt cgttgatcgt
     1021 ggcggtgccg accgcgaact gcagcttgct cgtcgcgatc ggattgacgg aaccgatggc
     1081 aggttcgacg ccgacgttgc ccgcgccgca tccggcggcc gctgcgacga tgctgacggc
     1141 gacgagccgc gcgatggcgc ggacgatgtt gcgatccatc agatcagacc tttatctgcc
     1201 agtagaagcg catcgtcagc ggcggctggt cgggcagtat gacgaagggg ctcgtcggga
     1261 acgcctcgcc cggatagccg ttcgggttcg cgccgcccga tatgccggtc gcgaccggct
     1321 cgaagtactg aaggttgacg agcggcacgc cgtagatctg gttgaaca
//
LOCUS       DASUCJ010000600         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_113509, whole genome shotgun sequence.
ACCESSION   DASUCJ010000600 DASUCJ010000000
VERSION     DASUCJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113509"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..>1087
                     /locus_tag="VFU90_07145"
     CDS             393..>1087
                     /locus_tag="VFU90_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479593.1"
                     /translation="MPDTVVVAAVRTPVGKLGGSLSSLAATTLGGEAIRVAVERSGVA
                     PSQIDHSIVGQVIQAGAGQAPHRQVTFIAGLPKTVTSETVNKVCASGTLAIASAHRLI
                     QAGAHRSIVAAGMESMSRAPYLVPEARFGYRMGDAKLIDSMVNDGLWDAFFQLTMAQE
                     SDRVASHLGLGRARQDEFALRSHHRAVDAFEKGRLADEMIGLKVASKARGRLIIEKIP
                     ASRELVGVGAMRI"
BASE COUNT          193 a          359 c          352 g          183 t
ORIGIN      
        1 aaggtcgacc gctccaaggt cgaccgctcc ccgggacatt tgttgggccg agcaagaatt
       61 ccgaatcgtg ggaccatctc ataggctatt ccgaacgccc gttggcttgt tggcgggagg
      121 accgcgatgc tacccttgga atgcacccat cccgccggta cgcttcgggc cggttctgcg
      181 gagatcaggc gagcgccgcc gcctgaatat cggcccgagc aaggtacaaa gtgtggcgat
      241 tttcgagaac gccggcgggg aaatgcaacc acggccgctg acaacagcgg ccgctttttt
      301 tatccgaccg aaggcgcgtc cggccaagtg ccgtaacgtg cgaaggtcga atcatcgcgc
      361 atcgcacgtt cccacgacag gagccttcat ccgtgcccga taccgttgtc gtcgccgccg
      421 tccgcacgcc ggtcggcaaa ctcggcggct ctctctcttc gctcgcagcg acgacactcg
      481 gtggtgaagc catccgcgtc gcggtcgaac gctccggcgt cgcgccgtcg cagatcgacc
      541 actcgatcgt cggccaagtg atccaagccg gcgccggaca agcgccgcac cgtcaagtga
      601 cgttcatcgc cggtctgccg aagacggtca cgtcggagac cgtcaacaag gtgtgcgcat
      661 ccggcacgct cgcgatcgca tcggcgcacc ggctcatcca agcgggcgcg catcgttcga
      721 tcgtcgcggc cggcatggag agcatgtcgc gcgcgccgta tctcgtcccc gaggcgcgct
      781 tcggctatcg gatgggcgac gcgaagctca tcgattcgat ggtgaacgac ggtctatggg
      841 acgccttctt ccagctcacg atggcgcagg agagcgatcg ggtcgcgagt catctcggcc
      901 tcggccgcgc gcggcaagac gagttcgcgc tgcgcagcca tcatcgcgcg gtcgacgcgt
      961 tcgaaaaggg ccgccttgcc gacgagatga tcggcctgaa ggtggcatcg aaggcgcgcg
     1021 gccggctcat catcgagaaa ataccggcgt cgcgcgagct cgtcggcgtc ggcgcgatgc
     1081 gcatcga
//
LOCUS       DASUCJ010000601         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_220440, whole genome shotgun sequence.
ACCESSION   DASUCJ010000601 DASUCJ010000000
VERSION     DASUCJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220440"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFU90_07150"
     CDS             <1..1109
                     /locus_tag="VFU90_07150"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU5479594.1"
                     /translation="VDIHAIGSGVGSLARVDSVSKTLSVGPESAGADPGPACYDKGGT
                     TATVTDADVVLGTINPDNFLGGRIRLDREKAVAAVGEIAAALGLSLMEAAAGVARIAE
                     FKMSDIIRKMTVEKGYDPRDFVLFAFGGAGPAHAGVFAYELGVEKVIVPQKAIASSWC
                     AFGAASADILHVYEQVDIQASPFDPARVNRMLEALEKKAHAQMDRDGVASPRRRFHFS
                     LDMRHRGQINEVEVPLPEKRLKAGFDAALRRRFAARYEQLYGRGSSYAEARTEIVTLR
                     LRASAATPRPRLVRTAKRSRIGAAALLGKRSIHWADLRRATTTPIYDGARLGSGNRIA
                     GPAVIETTDTTVVVHPGRSLFVDAFGNFEIRFGK"
BASE COUNT          197 a          416 c          396 g          191 t
ORIGIN      
        1 aggtcgacat ccatgcgatc ggctcgggcg tcggctcgct tgcgcgcgtg gacagcgtgt
       61 cgaagacctt gtccgtcgga ccggagtcgg cgggtgcgga tcccggtccc gcgtgctacg
      121 acaagggcgg cacgaccgcc acggtgaccg acgccgacgt cgtgctcggc acgatcaatc
      181 ccgacaactt cctcggcggg cgcatcaggc tcgatcgcga aaaagcggtc gcggccgtcg
      241 gagagatcgc cgccgcgctc ggtctctcgc tcatggaagc ggcggccggc gtcgcgcgca
      301 tcgccgagtt caagatgtcg gacatcattc gcaagatgac ggtggagaaa ggctacgacc
      361 cgcgcgactt cgtgctcttt gccttcggcg gcgccgggcc ggcccacgcc ggcgtgttcg
      421 cctacgagct gggcgtggag aaggtgatcg tcccgcagaa ggcgatcgcc tcctcctggt
      481 gcgcgttcgg cgcggcctcc gcggacatcc tccacgtcta tgaacaggtc gacatccagg
      541 cgagcccgtt cgacccggca cgcgtcaacc ggatgctcga ggccctggag aagaaggcac
      601 acgcgcagat ggaccgcgac ggcgtcgcgt ccccgcgccg gcgcttccac ttctcgctcg
      661 acatgcgcca tcgcggccag atcaacgagg tcgaagtgcc gcttcccgag aagcgattga
      721 aggccgggtt cgacgcggcg ctgcgtcgcc gtttcgccgc gcgctacgag cagctctacg
      781 gccgcggctc ctcctacgcc gaagcgcgga ccgaaatcgt cacgctgcgg ctgcgcgcct
      841 ccgccgcgac gccgcgcccg cggctcgtca ggacggcgaa acgttcgcgc atcggtgcgg
      901 cggctctgct cggcaagcgt tccatccact gggccgatct gcgccgcgcg acgaccacgc
      961 ccatctacga cggcgcgcgg ctcggttccg gcaaccgcat cgcgggaccg gccgtgatcg
     1021 agaccaccga cacgactgtg gtggtgcatc ccggacgctc gctcttcgtg gacgcgttcg
     1081 gtaatttcga gatcaggttt ggaaaatgaa cgacgttcat atcgcgccca aggcatcttc
     1141 aacgaagccg gtcttcgacg gcaagcgaac cggctacatt ccaccgaaga ggttgtccat
//
LOCUS       DASUCJ010000602         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1137628, whole genome shotgun sequence.
ACCESSION   DASUCJ010000602 DASUCJ010000000
VERSION     DASUCJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137628"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFU90_07155"
     CDS             complement(<1..1323)
                     /locus_tag="VFU90_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479595.1"
                     /translation="MSRYLASLIALPLFVFTTTAHAQSESALRKSDLVRYLTGTTYSK
                     SEIAAIVRRNCLAFVPSARDREDLKQLGANDAILREIDRCVNNNNKASSSESSDNPSR
                     PVAPAPASPPTLTVINGLASAMSGNVAYITVDLARGDTPMPGARLMLKGATNIPGGAQ
                     ADPVAITDAKGRATFTVPAGTHAGTYHMTVVTSDGSPLEGTTAVSLSTMPAGASLATV
                     NPTAIAIGAGARGTRELSVAVNDAVGNPVSKATVQVRPYPARSGVGTLTQITNDAGVA
                     KFSIQTQPLQAGDSLVVSVGDRAVASVRVTAAEQVTALLLEAERQLAIGRSGAIAAYD
                     SVLSVDPANTRALLGRGYAESAEGKFDAANKDFATALREGEDVSGAHTGLGYTALRRG
                     DLVGAAQHFQEALHSAAGDPAASTGLAYAELWRIDSRQAPHRADALSSP"
BASE COUNT          231 a          510 c          540 g          271 t
ORIGIN      
        1 cgggctcgag agcgcgtcgg cgcggtgcgg cgcttgccgc gagtcgatgc gccacagctc
       61 ggcgtaggcg agaccggtgg acgctgccgg atctccagcc gcggaatgca gcgcctcttg
      121 aaagtgctgc gctgcgccga cgagatcacc gcgccggagc gccgtgtatc cgagtccggt
      181 gtgcgcgccg gacacatctt cgccctcgcg cagcgctgtg gcgaagtcct tgttggcagc
      241 gtcgaacttt ccctccgccg actcggcgta gccgcggccc aggagcgccc gcgtgttcgc
      301 cggatccacc gagaggacgc tgtcgtaggc ggcgattgcc cccgatcgcc cgatggctaa
      361 ctgacgctcg gcttccagca gcagcgcggt cacttgctcg gccgccgtga cgcgcacgct
      421 cgcgacggcg cgatcgccga cgctcaccac caggctgtcg ccggcctgga gcggctgggt
      481 ttgaatcgag aatttggcca cgccggcatc gttcgtgatt tgcgtcagcg tgcctacccc
      541 tgagcgcgcg ggatagggcc gcacctgcac cgtcgccttg ctcaccggat tgccgaccgc
      601 gtcgttcacc gccacgctca gctcgcgcgt gccccgtgca ccggcgccga tcgcgatcgc
      661 cgtcgggttg accgtggcta acgatgcgcc ggccggcatc gtgctgagcg agactgccgt
      721 cgtgccctcg agcggcgagc catcggaggt gacgacggtc atgtgatacg tcccggcgtg
      781 cgtgccagcg ggcacggtga acgtcgcgcg tcccttcgcg tcggtaatgg cgacggggtc
      841 ggcctgcgcg ccgccgggaa tgttcgtcgc gcctttgagc atgagccgcg cgcccggcat
      901 cggcgtgtcg ccgcgggcca gatcgacggt gatgtacgca acgttaccgc tcattgccga
      961 cgccagcccg ttgatcaccg tgagcgtcgg cggcgatgcc ggcgccggcg cgaccggccg
     1021 cgatggattg tcggacgact ccgacgacga agccttgttg ttgttgttca cgcagcgatc
     1081 gatctcgcgc aggatcgcgt cgttggcgcc gagctgcttg agatcttcgc ggtcgcgcgc
     1141 cgacggaacg aaggcgaggc agttgcgtcg tacgatcgcc gcgatctcgc ttttcgagta
     1201 cgtggtgccc gtgagatagc gaaccaggtc gctctttctg agcgccgact cggactgcgc
     1261 gtgtgcggtc gtcgtgaaga cgaacagcgg aagagcgatc agcgaagcga ggtagcgcga
     1321 catggagtcc tgattggtgc gagagtccta cagtatttca gccgttctga ccggcggtgc
     1381 agagatcggc tgggttgccg catcccgaat caatcctcgt cggatacgga ccgggcgcaa
     1441 gtgaggtgcc acgaatcggg cccacgtgtg cggtcggcac ccgggcagta tcgagcatcg
     1501 aacacgttcg caccagtgaa cttagtcggt gcaagcgccg agctcgctct cg
//
LOCUS       DASUCJ010000603         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1397249, whole genome shotgun sequence.
ACCESSION   DASUCJ010000603 DASUCJ010000000
VERSION     DASUCJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397249"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFU90_07160"
     CDS             <1..1384
                     /locus_tag="VFU90_07160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5479596.1"
                     /translation="KLGEVVLVHAKDRPFRRVLAVGLGESAKFELSALARYAAVAVRF
                     LGRKNVTAFAVALPPQARGHELVAGSFVAEGALTGSFDTTVYQMAPEKAMAVENVTLL
                     KGDCDAAALERGVARGTILGEAVNLARRLAITPANDMTPTILARAAADVAKEASLEID
                     VLDEERARSEGMGSFLSVAQGSAQPPKFIVLRYTGDPSGKELLALVGKGITFDSGGIS
                     IKPADRMEEMKYDMSGGAGVIAAMSAIGKLKPKINVVGIVPATENMPGGKATKPGDIV
                     KAMNGKTIEVINTDAEGRLILADALCYANKLGATKIVDVATLTGAVVIALGHAAAAVI
                     SNDDAFVNDFLAVAKPSGERYWQMPYYDDYLGQMKSDIADLKNTGGRAAGTLTAAAFL
                     REFVDKTPWIHVDIAGTAYLDNESGWQAKGPTGIPVRTFVALAESLAGAPVIGNAVGN
                     GARKPAAVPT"
     gene            complement(1391..>1571)
                     /locus_tag="VFU90_07165"
     CDS             complement(1391..>1571)
                     /locus_tag="VFU90_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEU5479597.1"
                     /translation="LAVLSHGERVVVRAPDGAARLILVAGRPLHEPVARHGPIVMNTH
                     DELRQAFEDLRSGRF"
BASE COUNT          287 a          514 c          522 g          248 t
ORIGIN      
        1 aaagctcggc gaggtcgtgc tggtgcacgc gaaagaccgg ccgttccgcc gcgtccttgc
       61 ggtcggcttg ggagaaagcg cgaagttcga gttgtcggcg ctggcacggt atgcggccgt
      121 cgcggtgcgt tttctggggc gcaagaacgt gaccgctttt gcggtcgcgc ttccgccgca
      181 agcgcgcggg cacgagctgg tcgccggatc gttcgtcgcc gagggggcgc tgacgggttc
      241 gttcgacacg accgtctatc agatggcgcc cgagaaagcg atggccgtcg agaacgtcac
      301 gctgctgaag ggcgactgcg acgcggccgc gctcgagcgt ggcgtcgcac gcggtacgat
      361 tttgggcgaa gccgtcaatc tggcgcgccg gctggcgatt acccccgcca acgacatgac
      421 gccgacgatc ctcgcccgcg cggcggcaga cgtcgccaag gaggcgtcgc tcgagatcga
      481 cgtgctcgac gaggagcgcg cgcgcagcga aggcatgggg tcgttccttt cggtcgcaca
      541 gggcagcgcg cagccgccca agttcatcgt gctgcgctac accggcgatc cgtccggcaa
      601 agaacttttg gccttagtcg gcaagggcat taccttcgac agcggcggta tttccattaa
      661 gccggccgat cgtatggaag agatgaagta cgacatgtcc ggcggtgccg gcgtgatcgc
      721 cgccatgagc gcgatcggca agctcaagcc gaagatcaac gtcgtcggca tcgtcccggc
      781 gaccgaaaac atgccgggtg gaaaagccac caaacccggt gacatcgtca aggcgatgaa
      841 cggaaaaacc atcgaagtga tcaacaccga cgccgaaggg cggttgatcc tcgccgacgc
      901 gctgtgctac gccaacaaac tcggtgcgac caagatcgtc gacgtcgcca ccctcaccgg
      961 tgcggtcgtg atcgcgctcg gccatgcggc ggccgccgtt atctccaacg acgacgcctt
     1021 cgtcaacgat tttctcgccg tcgccaagcc gagcggcgag cgttactggc agatgccgta
     1081 ctacgacgat tatctggggc agatgaagag cgacatcgcc gacttgaaga acaccggcgg
     1141 acgcgccgcc gggactctta cggcggcggc gtttctgcgc gaattcgtcg acaagacgcc
     1201 gtggattcac gtcgacatcg ccggcaccgc gtatctcgat aacgaatccg gctggcaggc
     1261 caagggtccc accggaattc cggtccgcac cttcgtcgcg ctggccgaat cgctggccgg
     1321 cgccccggtc atcgggaacg cggtcggcaa cggcgcacgc aaaccggccg ccgtaccgac
     1381 ctaagccggc ttaaaagcgc ccgctgcgca gatcctcgaa cgcctgccgc agttcgtcgt
     1441 gggtgttcat gacgattggc ccgtggcgcg cgaccggttc gtgcaacggc cggccggcca
     1501 ccaggatcaa ccgcgccgcg ccgtccggcg cgcgcacgac gacgcgttcg ccgtgcgaca
     1561 gcacggctag c
//
LOCUS       DASUCJ010000604         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_923478, whole genome shotgun sequence.
ACCESSION   DASUCJ010000604 DASUCJ010000000
VERSION     DASUCJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923478"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1121
                     /locus_tag="VFU90_07170"
     CDS             <1..>1121
                     /locus_tag="VFU90_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479598.1"
                     /translation="ENLGFTDEQLADWSFSILRDGLGFTQQQIEEASDVICGRMTLEG
                     APHLKDEHLPVFDCANPCGKHGSRYIRPLAHIEMMAAAQPFVSGAISKTINLPQHATI
                     DDVKEAYRYSWERMIKAVALYRDGSKLSQPLAATYDFGGDVPEEEAALAPAPFQTPLQ
                     VAERIVYRYIAKRRRLPERRSGYTQKAIVGGHKVYLRTGEYEGGQLGEIFIDMHKEGA
                     AFRSLMNNFAIAVSLGLQHGVPLEEFVEAFTFTRFEPNGPVVGHDHIKMATSILDYIF
                     RELAVSYLGRYELAHVQPSMEMDAMGPKVEEEYVAEETGEVRYVPAAIREKLHPTSTH
                     LHPGHEHNPEETPHVVAEERRSERRDPSPVSSSTGVATV"
BASE COUNT          232 a          367 c          342 g          180 t
ORIGIN      
        1 aagagaatct cggcttcacc gacgagcagc tcgcagactg gagtttctcg attctgcgcg
       61 acggcctggg cttcacgcag cagcagatcg aggaagcctc ggacgtcatt tgcggccgca
      121 tgacgctgga aggcgcgccg catctcaaag acgagcatct gccggtcttc gactgcgcca
      181 atccgtgcgg caagcacggt tcgcgctaca tccgcccgct cgcgcacatc gagatgatgg
      241 cggcggcgca gccgttcgtg agcggcgcga tctccaagac gatcaatctg ccgcagcatg
      301 cgacgatcga cgacgtcaag gaagcctatc gctactcgtg ggagcgcatg atcaaagcgg
      361 tcgcgctcta ccgcgacggc tcgaagctct cgcagccgct cgccgcaacg tacgacttcg
      421 gcggcgacgt acccgaagag gaagcagcac ttgcgccggc gccgttccag acgccgctgc
      481 aggttgcgga acgcatcgta taccggtaca tcgccaagcg ccgccgtctg ccggaacgcc
      541 gcagcggcta cacgcaaaaa gcgatcgtcg gcggacacaa agtctacttg cgcacgggcg
      601 aatacgaagg cggccaactc ggcgagatct tcatcgacat gcacaaggaa ggcgccgcgt
      661 tccgctcgct gatgaacaac ttcgcgattg cggtctcgct gggcttgcag cacggcgtgc
      721 cgctcgagga gttcgtcgaa gcgttcacct tcacgcgctt cgagcccaac ggtccggtcg
      781 tcggtcacga tcacatcaag atggcgacct cgattctcga ctacatcttc cgcgaactgg
      841 ccgtcagcta tctcggacgc tacgagctcg cgcacgtgca gccgagcatg gagatggacg
      901 cgatgggccc gaaggtcgag gaagagtacg tcgccgagga aacgggcgaa gtgcgctacg
      961 ttccggctgc cattcgcgag aagctgcatc caaccagcac gcatttgcat cctggacacg
     1021 agcataaccc ggaagaaacg ccgcacgtcg tcgcggaaga acgccgcagc gagcgccgcg
     1081 atccgtcgcc ggtctcttct tccaccggcg tagccaccgt a
//
LOCUS       DASUCJ010000605         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_235514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000605 DASUCJ010000000
VERSION     DASUCJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFU90_07175"
     CDS             <1..190
                     /locus_tag="VFU90_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU5479599.1"
                     /translation="LLQIHDELIFEVAVEQRYDVARLVRRNMEEAIVLSVPLEVTVKS
                     GSNWYAVETLAEAESPRV"
     gene            183..635
                     /locus_tag="VFU90_07180"
     CDS             183..635
                     /locus_tag="VFU90_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU5479600.1"
                     /translation="MFRTAILAIAVFATAVAANAPSLPVVVVHAPKADLHLEVARTDP
                     EMERGLMYRTAIAPHTGMLFVFQTDAPVEFWMKNTLVPLDMIFIAADGTVRKIYAGVP
                     APAPNVSDEKIPREPGVAKYVIELGAGEAQRDGITEGVRLVVPGAAHA"
     gene            628..1440
                     /gene="mutM"
                     /locus_tag="VFU90_07185"
     CDS             628..1440
                     /gene="mutM"
                     /locus_tag="VFU90_07185"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5479601.1"
                     /translation="MPELPEVETIVRGLKRAIAGKRIARAEIRLPKIAVAPPGVAFAP
                     AVAGERVAGVRRRGKYAVLELASGRSLVTSLRMTGRLVVQADGEADFPGTHVVLWFGD
                     GLRLSFADVRTFGRMRLVEPAEAWDRELGAEPLSAGFTPEAFMGMLAGRTTPIKALLL
                     DQRRVAGIGNIYACEALWEARIRPSRPSKALTKPAIRRLHQAIVEVLKRAIAMRGTSV
                     DDYVDAGGLRGGFQNVLSVYGRSDRPCARCGKPIVRTVLAQRGTWWCRNCQR"
     gene            1582..>1808
                     /locus_tag="VFU90_07190"
     CDS             1582..>1808
                     /locus_tag="VFU90_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5479602.1"
                     /translation="MLTGTIVQKDKDELLVDVGGKSEGVLPFRELSLAIDPRLLKIGD
                     TLEVMIHRIDDMDGTLFLSERRARALKTWEKV"
BASE COUNT          343 a          554 c          600 g          311 t
ORIGIN      
        1 gctgctgcaa attcacgacg agctcatctt cgaagttgcc gtcgagcagc gctacgacgt
       61 ggcgcgcctg gtgcgccgga acatggagga agcgatcgtg ctttcggtac cgctcgaagt
      121 caccgtaaag tcgggttcga actggtacgc ggtcgagacc ctcgcggaag cggagagtcc
      181 tcgtgtttag aacggcgatt ctggcgattg ccgtattcgc gaccgcggtc gctgcgaacg
      241 cgccgtcgct gccggtggtg gtggtgcacg ctcccaaggc agaccttcat ctcgaagtgg
      301 cacggacgga tccggagatg gagcgcggct tgatgtaccg caccgcgatc gcgccgcaca
      361 ccgggatgct gttcgtcttt caaaccgacg caccggtcga gttctggatg aagaacacgc
      421 tggtaccgct cgacatgatc ttcatcgcgg ccgacggaac cgtccgcaaa atctatgccg
      481 gcgttccggc gcccgcgccc aacgtttcgg acgaaaagat tccgcgcgag ccgggcgtcg
      541 cgaagtacgt gatcgaactc ggcgccggcg aggcgcagcg cgacggcatc acggagggag
      601 tgcggctcgt cgttcccggc gccgcacatg cctgagcttc ccgaagtcga gacgatcgtc
      661 cgggggctga aacgcgcgat cgccggcaag cgtatcgcgc gcgccgagat ccgtctcccc
      721 aagattgccg tcgcaccgcc cggagtcgcc ttcgcgccgg cagtggccgg cgaacgcgtc
      781 gccggcgttc ggcgccgcgg gaagtacgcc gtgctcgagc tcgcatccgg acgcagcctg
      841 gtcaccagtc tgcggatgac cgggcggctc gtggtgcagg ccgacggcga agccgacttt
      901 cccggcacgc acgtggtcct gtggttcggc gacggactgc gtttgagctt cgcggacgtg
      961 cggacgttcg gacgtatgcg gctggtcgag ccggccgaag cgtgggaccg ggaactcggt
     1021 gcggagccgt tgtctgcagg ctttacaccc gaggccttta tgggtatgct tgcggggcgg
     1081 acgacgccga tcaaggcatt gctgctcgat cagcggcgcg tcgcgggcat agggaacatc
     1141 tatgcgtgcg aggcgctgtg ggaagctcgg attcgtccga gccgtccctc gaaggcgttg
     1201 acgaaaccag ccattcgccg gttacaccag gccatcgtcg aggttttgaa acgcgcgatt
     1261 gcaatgcgcg ggacgagcgt cgacgattac gtggacgccg gcgggctccg gggaggattt
     1321 caaaacgtcc tctcggtgta cggccggagc gaccggccct gcgcccgatg cggtaagccg
     1381 atcgtgcgaa cggtcctcgc ccaacgcgga acctggtggt gtcgcaactg tcaacggtga
     1441 cgtctgcaaa aaatacaaaa cgtaaggtag gatctcaaca tctcaacgac tcaaaccgct
     1501 tccccgaata tagaagaaga ccaactagca ctcgaacagc agctctacga agagtcgctg
     1561 aaggtcctcg acgagggcca agtgctcacc ggcacgatcg tccagaagga caaagacgaa
     1621 ctgctggtcg acgtcggcgg taaatccgaa ggcgtgctcc ccttccgcga actctcgctc
     1681 gcaatcgatc cgcggctgct caagatcggc gatacgctcg aggtgatgat tcaccgtatc
     1741 gacgacatgg acggaacgct gttcctgtcg gagcggcgtg cccgcgcgct caagacctgg
     1801 gagaaagt
//
LOCUS       DASUCJ010000606         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_999943, whole genome shotgun sequence.
ACCESSION   DASUCJ010000606 DASUCJ010000000
VERSION     DASUCJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999943"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFU90_07195"
     CDS             <1..209
                     /locus_tag="VFU90_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479603.1"
                     /translation="VQYEFGSLLKFTESTFALGSLHTTDARANDLSDAFDFTQSPRRF
                     VTIKAPPFSPSPDALEGSRNVEDP"
     gene            211..>1112
                     /locus_tag="VFU90_07200"
     CDS             211..>1112
                     /locus_tag="VFU90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479604.1"
                     /translation="MIRRAFACAIAVAVAACGGHSPGAALPQTAPAPAEFANRGLPTP
                     LRHIVIVVQENRSFENFFAGFPGADAPMTGKTHDGKTVRLKPITFAKVSLNHFYKDGR
                     IGYNHGRMNGFDRVPPMQPFGKLEPYSYVERSLIRPYWTMAQQYVLADHMFPTEWGPS
                     FTAHLNLVAGTTVIRRGVAIVDMPTLDGAVKGGGCDADPGTVTSLLTAQGEYRQNEGP
                     FPCFSFQTLAGVLDPGHVSWKYYAAYWPNGKTLWNAFDAIRAVRYGPDWKQHVVKTPK
                     QILTDAAAGKLPAVSWVIPTMPDS"
BASE COUNT          205 a          371 c          344 g          192 t
ORIGIN      
        1 acgtgcagta cgagttcggc agtttgctta agtttacgga atcgaccttt gcactcggtt
       61 cgctgcacac taccgacgcc cgcgccaacg atctctccga cgcgttcgac tttacccaga
      121 gcccgcgccg gttcgtcacg atcaaagcgc cgccgttcag ccccagcccc gacgcgcttg
      181 agggcagccg caacgtcgaa gatccgtagc gtgatacgcc gcgcgtttgc gtgcgcgatt
      241 gccgtagcgg tcgccgcctg cggcgggcac tcgccgggcg cggcactgcc gcaaacggcg
      301 ccggcgccgg ccgagtttgc aaatcgcggg ctgccgacgc cgcttcgcca tatcgtgatc
      361 gtcgtacagg aaaaccgcag cttcgaaaac ttttttgctg gtttccccgg cgccgatgcc
      421 ccgatgaccg gcaagacgca cgacggcaag acggttcggc tcaagccgat cacgtttgca
      481 aaagtctcgc tcaaccactt ttacaaagac gggcgcatcg gctacaacca cgggcgtatg
      541 aacgggttcg atcgcgtgcc gccgatgcag ccgttcggca agctcgaacc ctactcgtac
      601 gtcgagcgtt cgctgatccg gccgtactgg acgatggcgc agcagtacgt cctggcagat
      661 cacatgttcc caaccgaatg ggggccgagc ttcaccgcgc atctgaacct ggttgccggg
      721 accacggtga tccgccgcgg cgttgcgatc gtcgacatgc cgacgctcga cggcgcggtg
      781 aagggcggcg gctgcgacgc cgaccccgga accgtgacca gcctgttgac cgcccagggc
      841 gagtaccggc agaacgaagg gccgtttccg tgcttttcgt tccaaacgct ggccggcgtg
      901 ctcgaccccg ggcacgtgtc gtggaaatat tacgcggcgt attggccgaa cgggaagacg
      961 ttgtggaacg ccttcgacgc cattcgagcg gtgcgatacg gacccgactg gaagcagcac
     1021 gtcgtcaaaa cgcccaagca gatcctcacc gacgcggcgg cggggaaact cccggcggtc
     1081 tcgtgggtga tccccaccat gcccgattcc ga
//
LOCUS       DASUCJ010000607         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_831663, whole genome shotgun sequence.
ACCESSION   DASUCJ010000607 DASUCJ010000000
VERSION     DASUCJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831663"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFU90_07205"
     CDS             <1..288
                     /locus_tag="VFU90_07205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5479605.1"
                     /translation="RPLLIARLYAAMLAATQLLRPLLFAFSRSREWAADRFALAATAD
                     ARTGAASLRRLRDCNLADEDPPGWYELFFASHPSLKARIAALENSRTPLSS"
     gene            complement(378..659)
                     /locus_tag="VFU90_07210"
     CDS             complement(378..659)
                     /locus_tag="VFU90_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479606.1"
                     /translation="MPKVGRVELRISQIEGFLVKIHHLDGRDVRSDRQGMPSWPYERA
                     AKDSWTVADWRRERFNAVYPGFDVDVLDGDCNPVPGQTRLENLRESYDD"
     gene            complement(670..>1252)
                     /locus_tag="VFU90_07215"
     CDS             complement(670..>1252)
                     /locus_tag="VFU90_07215"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HEU5479607.1"
                     /translation="DARGTRRVTGVEAEGMTACPCAQAMMREHSLRELLEAGFSDADA
                     RRALDALPVATHNQRGRGTLLIGCAGEPDDAVRAEDLVEIVENAMSSETYDLLKRPDE
                     FFIVNKAHHKPRFVEDVVRGILAGALDMYADFGDDTFAFAGQRNYESIHKHDAFAEAF
                     GRFGELRDELRTGSRAASKTELDGWLRDRDARP"
BASE COUNT          189 a          430 c          409 g          224 t
ORIGIN      
        1 cgcccgttgt tgattgcccg cctctacgcc gcgatgctcg ccgcgaccca gctgctgcgc
       61 ccgttgctgt tcgcattttc ccgctcgcgc gaatgggccg ccgaccggtt tgcgctcgcg
      121 gcgaccgccg acgcccgcac cggcgccgcc tcgctgcggc ggctgcgcga ctgcaatctc
      181 gccgacgagg atccacccgg ctggtacgag ctgttcttcg cctcgcatcc gtcgctcaaa
      241 gcccgcatcg ccgccctcga aaactcccgt accccgttgt catcctgagc ggagcctgcg
      301 aagcaggcgc agtcgaaggg ctgagcggag cgccgcaggc gcgcagtcga aggacgagtg
      361 cgacgtagcg ctacagtcta atcgtcgtac gattcgcgga gattttcgag gcgggtttga
      421 ccgggaacgg ggttgcagtc gccgtccagc acgtcgacgt cgaagccggg gtagaccgcg
      481 ttgaagcgct cgcggcgcca gtcggcgacc gtccacgaat ctttggcggc gcgctcgtac
      541 ggccagctcg gcatgccttg gcggtcggag cgaacgtcgc gaccgtcgag atggtggatt
      601 ttgaccagaa aaccttcgat ttgagagatg cgcagctcga cgcggccgac tttcggcatc
      661 gactcctcct tacggccgcg cgtcccggtc gcgcagccag ccgtcgagtt ccgtcttgga
      721 agccgcccgg ctgccggtgc gaagctcgtc gcgtagctcg ccgaaacggc cgaacgcttc
      781 ggcgaaggcg tcgtgtttgt gaatggactc gtagtttcgc tgaccggcga acgcaaacgt
      841 gtcgtcgccg aagtcggcgt acatatcgag cgcccccgcg agaatcccgc gtaccacgtc
      901 ctcgacgaac cgcggcttgt ggtgcgcttt gttgacgatg aagaactcgt ccggacgttt
      961 gagcagatcg taggtttcgc tcgacatcgc gttctcaacg atctcgacca gatcttcggc
     1021 ccggacggca tcgtcgggtt cgccggcgca gccgatcagc agcgtcccgc ggccgcgctg
     1081 gttatgggtg gcgaccggga gcgcatcgag cgcgcgccgc gcgtcggcat ccgagaaccc
     1141 ggcttcgagc aactcgcgca gcgaatgctc gcgcatcatc gcctgcgcgc acgggcaggc
     1201 ggtcattcct tcggcttcca ctccggtcac gcgccgcgtc ccgcgcgcgt cg
//
LOCUS       DASUCJ010000608         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1152566, whole genome shotgun sequence.
ACCESSION   DASUCJ010000608 DASUCJ010000000
VERSION     DASUCJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152566"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..2722)
                     /gene="galA"
                     /locus_tag="VFU90_07220"
     CDS             complement(191..2722)
                     /gene="galA"
                     /locus_tag="VFU90_07220"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HEU5479608.1"
                     /translation="MSESKMSRRDALLTGAASVLGLVSIPSPLDALRSGTDRVVGTTP
                     ARASLARGDAGISPAVAGGGRERIRFDTDWRFHLGHASDPARDFGYGADEEFAKSGEL
                     FAPVSHADFDVSSWRRLDLPHDWAVELPFVDEHGLTSYGFKPVGRAHPDTSIGWYRKS
                     FAVPAEDLGRRISLEFDGVFRDCMVALNGHLVARNLSGYAPFAVDITPELNYGGDNVL
                     VVRVDATEHEGWFYEGAGIYRHVWLVKTAPVHVALWGTFVRATVDGAKRSATAHITTT
                     IQNDGDADARCAIELRMLDGAGQVVQTSRSDPVTIPAAGSSAVDHAMRIDAPSLWSLD
                     APHLYTLVTLLTVDQVEVDRTETPLGIRTVQFDPDRGVLVNGEHVEIRGTCNHQDHAG
                     VGSALPDRLQAYRIERLKAMGANAYRTSHNPPAAELLDACDRIGMLVLDETRMFGADP
                     EALSQLGRLVRRDRNHPCVFAWSLANEEDGEQGTDRGARMAEVMKATVRQLDPTRPVT
                     AAMNGDWGKGFTGVIDVQGFNYRVGREMDAFRARFPRKPAMGTEVASTVSTRGIYAND
                     TARGYVSAYDRNFPPWASTAEAWWTAYLARPWASGGFVWTGFDYRGEPTPYAWPCINS
                     HFGIMDTCGFPKDNYYYYQAQWGSAPVLHVFPHWNWAGHEGQPIEVWCHTNLERVELF
                     LNGARVGGGAERVPPNGHAAWHVPFAPGVLEARGYRGGRVVLTARRETAGAPAAVALE
                     ADRIRLTADGMDVSVVAARIVDARGRTVPTASNRVTFGVTGPLAVIGVGNGDPSSHES
                     DKDTSRSAFNGLCCAIVQAGTERGPAKVTAAADGLRGSAIDVVVG"
     gene            2810..>3159
                     /locus_tag="VFU90_07225"
     CDS             2810..>3159
                     /locus_tag="VFU90_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479609.1"
                     /translation="MAGSGVRPDTAAAAGDRLTKNAPLALRVATMADVSALNAMIAES
                     ARALSAGYYAPAQVEALVTHVFGVDTHLVTDGTYYVIDAPDGAPAAAGGWSSRRALYG
                     GDQMASHDTTDERL"
BASE COUNT          505 a         1111 c         1039 g          504 t
ORIGIN      
        1 gccgctgctg tcgctcatct tccagttcga gaacggagga gggatcaccg ggcagttcac
       61 cggaacgctc accgcgccga atcacctcca ggggtccgtc atcttctacg ggatcaccca
      121 gacgccgcaa acggccacgg cgtcgtacac caggcggtga tcgcagatcc gccggcgtgg
      181 tgatcgctgc ctaaccgacg acgacgtcga tcgccgaccc gcgcaatccg tccgccgccg
      241 ccgtcacctt cgccggtccg cgctccgttc ccgcctgcac gatcgcgcaa cagagcccgt
      301 tgaacgcact gcgcgacgta tccttgtcgc tctcgtgcga gctgggatcg ccgttcccca
      361 caccgatcac cgccagcggt cccgtgacgc cgaaggtcac gcggttcgat gccgtcggca
      421 cggtccggcc cctggcgtcg acgatgcgcg ccgcgaccac cgacacgtcc atgccgtctg
      481 ccgtcagtcg aatccggtcg gcctcgagcg cgacggcggc cggcgccccg gcggtctcgc
      541 ggcgcgccgt gagcacgacg cggccgccgc ggtagccgcg cgcctcgagg acgccgggcg
      601 cgaacggcac gtgccacgcc gcgtgtccgt taggcggcac gcgctccgcg ccgccgccga
      661 cgcgcgcgcc gttcaggaac agctcgacgc gctcgagatt cgtatggcac cacacctcga
      721 tcggctgccc ttcgtgtccg gcccagttcc agtgcgggaa aacgtggagc acgggcgccg
      781 agccccactg tgcctgatag tagtagtagt tgtccttcgg aaagccgcac gtatccatga
      841 tgccgaagtg cgaattgatg cagggccacg cgtacggcgt gggctcgccg cgataatcga
      901 atcccgtcca cacgaatcct cccgacgccc acggccgggc gaggtacgcc gtccaccacg
      961 cctccgccgt cgacgcccag ggcgggaaat tccggtcgta cgccgatacg tagccgcgcg
     1021 cggtgtcgtt cgcgtagatc ccgcgcgtcg acacggtgct cgccacctcg gtgcccatcg
     1081 ccggcttgcg cgggaagcgc gcgcgaaacg catccatctc gcgcccgacg cgataattga
     1141 agccctgcac atcgatcacg cccgtgaagc ctttccccca gtcgccgttc atcgccgccg
     1201 tcaccggccg cgtcggatcg agctgcctaa cggtggcctt catcacctcc gccatgcgtg
     1261 cgccgcggtc ggtgccctgt tcgccgtctt cctcgttggc cagcgaccag gcgaagacgc
     1321 acgggtgatt tctgtcgcgg cgcaccagac gaccgagttg gctcaacgcc tccggatccg
     1381 cgccgaacat gcgcgtctcg tccagcacca gcatgccgat ccggtcgcag gcgtcgagca
     1441 gctcggcagc cggcggattg tgcgacgtgc ggtacgcgtt ggcgcccatc gccttgaggc
     1501 gctcgatccg gtaggcctgc aggcggtcgg gcagggcaga gccgacaccg gcgtgatcct
     1561 ggtgattgca cgtgccgcga atctcgacgt gttcgccgtt cacgagaacg ccgcggtcgg
     1621 ggtcgaattg cacggtgcga atgcctaacg gcgtctccgt gcgatccact tccacctggt
     1681 ccacggtgag cagcgtgacg agcgtgtaga gatggggggc gtcgagcgac cacagcgacg
     1741 gcgcatcgat gcgcatcgcg tgatcgacgg cgctggaacc ggccgccgga atcgtgaccg
     1801 gatccgatct cgaggtttgt acgacctgtc cggcgccgtc gagcatccgc aattcgatgg
     1861 cacaccgcgc gtcggcgtcg ccgtcgttct ggatggtcgt cgtgatgtgc gccgtggcgc
     1921 tgcgcttcgc gccgtccacc gtcgcgcgaa cgaacgtgcc ccagagcgcc acgtgcaccg
     1981 gcgccgtctt gacgagccac acatggcgat agattccggc gccctcgtaa aaccaccctt
     2041 cgtgctcggt cgcatccacg cgcaccacga gcacgttgtc gccgccgtag ttcagctctg
     2101 gtgtgatgtc caccgcgaac ggggcgtacc cgctcagatt gcgcgccacc agatggccgt
     2161 tgagcgcaac catgcaatcg cgaaacaccc catcgaactc gagtgaaatg cgtcggccca
     2221 ggtcctcggc gggaaccgcg aacgacttgc gataccagcc gatgctcgtg tcgggatgtg
     2281 cgcgtcccac cggcttgaac ccgtaactcg ttaggccgtg ctcgtcgacg aacggcagct
     2341 ccacggccca gtcgtgcggc aggtcgaggc gccgccatga cgagacatcg aagtcggcgt
     2401 gcgagacggg agcgaacagc tcgcccgact tggcgaactc ttcatcggcg ccgtagccga
     2461 agtcgcgggc gggatctgat gcgtgtccca gatgaaagcg ccagtccgtg tcgaatctga
     2521 tgcgttctcg cccgccgccc gcgaccgccg gcgagattcc ggcgtcaccg cgcgcgagcg
     2581 acgcccgcgc gggcgtggtc ccgacgacgc gatcggtgcc gcttcgcagg gcgtcgagcg
     2641 gcgatgggat gctgacgagg ccgaggacgg aggctgctcc tgtgagcagg gcatcgcggc
     2701 gggacatctt ggactcggac atggttccgt gtggttggtc gcgacgcata actttcaacc
     2761 aatgcgcggg cctgacaagc gatcagcggg agacctgccc cggaacgagg tggccggctc
     2821 cggcgtgcgt cccgatacgg cagccgcggc cggcgatcgc ctaacaaaga acgcgcctct
     2881 cgcgctccgt gtggcaacga tggccgacgt gtcggcgctc aacgccatga ttgcggaatc
     2941 cgctcgggca ctgagcgcag gctattatgc gcctgcgcag gtcgaggcgc tggtgacgca
     3001 tgtgtttggc gtcgacacgc atctcgtcac cgacggcacg tactacgtga tcgacgcgcc
     3061 ggacggcgcg cccgccgcgg cgggcggctg gagctctcgt cgcgccctgt atggtggcga
     3121 tcagatggcg tcgcacgaca ccacagatga gcgactcga
//
LOCUS       DASUCJ010000609         1405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1275156, whole genome shotgun sequence.
ACCESSION   DASUCJ010000609 DASUCJ010000000
VERSION     DASUCJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275156"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFU90_07230"
     CDS             <1..637
                     /locus_tag="VFU90_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5479610.1"
                     /translation="HALNTLLRDDAIVTTDSGTITTWIARHLMIRGEMMFSCSGNLAT
                     MACGLPYAIAAAVAHPGRQVVAFVGDGGLTMLLGELATCVKYGLDVKVVVIKNNVLGQ
                     IKWEQMVFLGNPEYVCELQPIDFATVARGFGVKGYVADDPKTCGDVLREALATPGPAL
                     VEAVVDPHEPPMPPKATLKQVSNLARSLAEGTPAGNKIALTVASDIVRELV"
     gene            643..>1405
                     /locus_tag="VFU90_07235"
     CDS             643..>1405
                     /locus_tag="VFU90_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5479611.1"
                     /translation="MSGSSPRIERVSAATYRIPTDAPEADGTIAWDATTLVVADVAAA
                     GQSGMGYAYADAGSAGIIEHTLCNVLTGKDAFDLPALWSAMVAAVRNIGRDGVAMGAI
                     SALDVAMWDLKARLLDVGLVDLFGRARESVPIYGSGGFTSYSNERLATQLSGWTGKEG
                     CRWVKMKIGTEPAADCERVRVAREAIADAGLFVDANGAYSRKQALDFAERFAGLGVSW
                     FEEPVSSDDLEGLRLLRDRAPAGMEIAAGEYGYNVF"
BASE COUNT          238 a          436 c          499 g          232 t
ORIGIN      
        1 gcatgcgctg aacacgctgt tgcgcgacga tgctatcgtt accaccgatt ccggtacgat
       61 caccacgtgg atcgctcgcc acctgatgat ccgcggcgaa atgatgttct cgtgctcggg
      121 caaccttgcc accatggcct gcgggctacc ctacgcgatt gcagcggccg tggcgcatcc
      181 cggccgacag gtggtcgcat tcgtcggcga cggcgggctg acaatgctgc tgggcgagct
      241 cgcgacctgc gtcaaatatg gactggacgt gaaggtcgtg gtcatcaaga acaacgttct
      301 gggccagatc aagtgggaac agatggtgtt cctgggcaat ccggaatatg tctgcgagct
      361 gcagccgatc gatttcgcga cggtggcgcg cggcttcgga gtcaagggct atgtggccga
      421 cgatcccaag acctgcggcg acgtgctgcg cgaggcgctg gcgacgccgg gtccggcgct
      481 ggtcgaggcc gtggtcgatc cgcacgaacc accgatgccc cccaaggcga ctctcaagca
      541 ggtctcgaac ctcgcccgtt cgcttgcgga aggcacgccg gcgggcaaca agatcgcgct
      601 gaccgtcgcc tcggacatcg tccgcgaact cgtctagcga cgatgtccgg ctcgtctcct
      661 cggatcgagc gcgtcagcgc cgcgacttat cgcattccga ccgatgcgcc cgaggcggac
      721 ggcacgatcg cgtgggacgc cacgacactg gtggtcgccg acgtcgcggc ggccggccag
      781 agcggcatgg ggtatgctta cgccgacgcg ggaagcgcag gcatcatcga gcacacgctg
      841 tgcaacgtgc tgacgggcaa ggacgctttc gatctcccgg cgctatggtc ggcgatggtc
      901 gcggcggtcc gcaacatcgg ccgcgacggc gtcgcgatgg gagccatctc ggcgctcgac
      961 gtcgcgatgt gggacctgaa ggcgcggctg ctcgacgtcg gccttgtcga cctgttcggg
     1021 agagcgcggg agagcgtgcc gatctacggc agcggcggat tcacatcgta ctcgaatgag
     1081 cggctcgcga cacagctcag tggctggacg ggcaaggagg gttgccgctg ggtaaagatg
     1141 aagatcggca ccgagccagc ggcggactgc gagcgggtgc gcgtggcacg ggaggcgatc
     1201 gccgacgcgg gtctgttcgt cgacgccaat ggcgcgtact cgcgcaagca ggcgctcgat
     1261 ttcgcagagc ggttcgccgg gctcggcgtc agctggttcg aagagccggt ctcgagcgac
     1321 gacctcgagg gactgcggct gttacgcgac cgcgccccgg ccggcatgga aatcgcggcg
     1381 ggcgagtacg gctacaacgt ctttg
//
LOCUS       DASUCJ010000610         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_495462, whole genome shotgun sequence.
ACCESSION   DASUCJ010000610 DASUCJ010000000
VERSION     DASUCJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495462"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1763)
                     /locus_tag="VFU90_07240"
     CDS             complement(<1..>1763)
                     /locus_tag="VFU90_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479612.1"
                     /translation="ASVAIGQAQRGGGRQPKTAQPAVEPRATKHPKRWIALVGLGGML
                     LLAVVVALALEPVRQSIFGWQRHLPEIVQLPFDLLWLGVIAIVVAGFMAPLETLGWWA
                     GWYGDEIDTSEVTRPSRHAAGAAAVSRYVVYLDGIAQSSSRYTPDIETFLDALGPALP
                     ADVKLVRGVMAYSVLNRPLDDDPILSNFWKLVDASRSTHMNSLLGMFVNLRNVTIVAV
                     SADPRYGPMYNFGIADVIYRSLIANGYEKKSGTPVTLIGYSGGGQMACGAASFLKRAL
                     DAPVDVISLGGVISGNDPILQLEHLYHLVGDKDRVERIGPVMFASRWPIAPLSYWNRA
                     KRLGTLSCVSLGPVAHQVPGGLLDPDARLPDGRSYLQQTLEYIHSILTDRFAAKTARP
                     RLPSNYQRYVEGTGELWGALASSRCPAATADRFLPVAQWMGRLILPEREARFGGVWFE
                     VLNAPPEHAHLLGTRVRLRWSESPAVRRRVRAAVRDVNFSAEARSASEYGGLVMPVRL
                     NRWRLVDPLESLAGAHPIDDVVVKLGGEVRASAAGGEVTLRIEREPIQITGTHYALVS
                     FRGPAPRTADGFRAVHFDRSA"
BASE COUNT          312 a          601 c          576 g          274 t
ORIGIN      
        1 ggcggagcga tcgaagtgaa cggcgcgaaa gccgtcggcc gtgcgcggtg ccgggccgcg
       61 aaacgacacg agcgcgtaat gcgtgccggt gatttgaatc ggctcgcgct cgatccgcag
      121 cgtgacctcg ccgccggcgg cgctcgcgcg gacctctccg cccagcttta cgacgacgtc
      181 gtcgatcggg tgtgcgccgg ccagcgattc gagcggatcg accagccgcc aacggttcaa
      241 gcgcaccggc atcacgaggc cgccgtactc gctggccgag cgcgcctcgg cgctgaagtt
      301 cacgtcgcgc accgccgcgc gaacgcgccg ccggacggcc ggactctccg accagcgcaa
      361 ccgcacgcgc gttcccagca gatgcgcgtg ttcgggcggc gcattcagca cctcgaacca
      421 gacgccgccg aagcgcgcct cgcgctccgg caggatgagc cggcccatcc actgcgccac
      481 gggcaagaac cggtccgccg tcgcggccgg acaacggctg gaggccagcg ccccccagag
      541 ttctccggtc ccctcgacgt accgttgata gttactggga aggcgcggcc gcgccgtttt
      601 cgccgcgaac cggtcggtca ggatgctgtg aatgtattcg agcgtttgtt gcagatacga
      661 gcgcccgtcc ggcaatcgcg cgtcgggatc cagcaagccc cccggcactt gatgcgcgac
      721 cgggccgagc gagacgcagg agagcgttcc gaggcgcttg gcgcggttcc aatacgacaa
      781 cggcgcgatc ggccagcgcg aagcgaacat caccggaccg attcgctcga cgcgatcctt
      841 gtcgccgacc agatggtaga gatgttcgag ttgcaggatc gggtcattgc cgctgatcac
      901 tccgcccagc gagatcacgt cgaccggagc gtcgagcgcg cgtttgagga acgacgccgc
      961 accgcacgcc atctgcccgc cgccgctata accgatcaac gtcaccgggg taccgctctt
     1021 cttttcgtag ccgttggcga tcagggagcg gtagatgacg tcggcgattc cgaagttgta
     1081 catcggcccg tagcgcggat ccgccgacac ggcgacgatc gtgacgttgc gcaggttgac
     1141 gaacatcccg agcagcgaat tcatgtgcgt gctgcggctc gcatccacca gcttccagaa
     1201 gttcgataga atcggatcgt cgtcgagcgg acggttcaaa accgaatacg ccatcacgcc
     1261 gcgcacgagc ttgacgtcgg ccggcagcgc gggtccgagc gcgtccagga acgtttcgat
     1321 gtcgggtgtg taccggctgc tcgattgcgc gatgccatcg agataaacca cgtaacgcga
     1381 gaccgctgcg gcgccggcgg cgtgacgcga cggacgagtg acttcgctcg tatcgatttc
     1441 gtcgccgtac catccggccc accagccgag cgtttcgagc ggcgccatga aaccggccac
     1501 cacgatcgcg atcacgccga gccatagcag gtcgaacggc agttgaacga tctcgggaag
     1561 atgccgctgc caaccgaaga tactctgacg aaccggctcg agggcgagcg ctacgacgac
     1621 cgcgagcagc agcatgccgc cgagtccgac cagcgcgatc cagcgcttcg ggtgtttcgt
     1681 cgcacgcggt tcgaccgccg gctgcgccgt tttcggctgc cggccgccgc ccctttgagc
     1741 ttggccgata gctacgctcg cgt
//
LOCUS       DASUCJ010000611         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_159477, whole genome shotgun sequence.
ACCESSION   DASUCJ010000611 DASUCJ010000000
VERSION     DASUCJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="ftsA"
                     /locus_tag="VFU90_07245"
     CDS             <1..649
                     /gene="ftsA"
                     /locus_tag="VFU90_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5479613.1"
                     /translation="ATLAPEEKQVGVVLLDIGGGTTDIAVYSLGSAIYTATLPVGGNI
                     LTNDISLGLKTTLAEAEDVKKRFGAAVRDAGGEERFSVRTLDGRSSKDYSTEQLRQIV
                     VPRVLETLRMAKQKIIDNVPRDLVLGEVVVTGGGALLPDIESLAEDVFGLPVRVGMPN
                     TIGGLTDAIALPQYSTAIGLVLFGASDERDAADMGRRRGGSIVHRLQKWLSDLWN"
     gene            925..2007
                     /gene="ftsZ"
                     /locus_tag="VFU90_07250"
     CDS             925..2007
                     /gene="ftsZ"
                     /locus_tag="VFU90_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5479614.1"
                     /translation="MAEGRRSNTDPQHAATIKVIGIGGGGCNAVNRMVRAGLAGVDFF
                     SVNSDVQALRSSATENTVHIGGGQTRGLGAGANPTLGREAAEESREDLALILDGADLV
                     FLTAGMGGGTGTGAAPVIAEIAREAGALTIAVVTKPFAFEGKKRMQVAEHGIVELESK
                     VDTLITIPNERILQVIEKKTPLNEAFAYADDVLRQGIQGISDLITQPGLINLDFADVK
                     TIMTDAGSAMMGIGEGSGEHRAADAAQKAIASPLLETTIEGARGVIFNITGGPDMAMY
                     EVNEAAEMISRAVDPDAQIIFGASVDPSMSGKVRVTVLAAGFGAPRGYRDTQSGYAFD
                     KTGKLETVAPVNMDDIEVPAFLRYRG"
BASE COUNT          414 a          673 c          674 g          317 t
ORIGIN      
        1 ggcaacgctg gcaccggaag agaagcaggt cggcgtcgtg ttgctcgaca tcggcggcgg
       61 caccaccgat atcgccgttt actcgctcgg cagcgcaatt tatacggcga cgctccccgt
      121 cggcggcaat atcctcacca acgacatctc actgggcctc aaaacgaccc ttgccgaagc
      181 cgaggacgtc aagaagcgct tcggcgccgc ggtacgcgac gccggcggcg aagaacggtt
      241 ttcggtacgg acgctcgacg gccgctcgag caaggactat tcgaccgagc agctgcggca
      301 aatcgtggtg ccgcgcgtgc tcgagaccct gcggatggca aagcagaaga tcatcgacaa
      361 cgtgccgcgc gacctggtac tcggcgaagt cgtggtaacc ggcggcggtg cgttgcttcc
      421 cgacatcgaa tccctggccg aagacgtttt cggtctgccg gtacgggtgg gcatgccgaa
      481 cacgatcgga ggactaaccg acgcgatagc gcttccgcaa tactcgacgg caatcgggct
      541 cgtgctcttc ggtgcgagcg acgagcgcga tgccgccgac atggggcggc gccgcggcgg
      601 ttccatcgta catcgcctgc agaaatggtt gtcggactta tggaactgaa acctcgcaaa
      661 ccccgtaaac gcgtctcgcg gccgcacgta ccgctcgacg aggtcgcgac cgcggagcta
      721 cctgccgacg tgcccgagcc gctcgtcgcg acggagccga tcgcatcgcc gccgctcgag
      781 gagccggccg tcgccgcgca cgcggatgcg acggacgacg atttcgaacc ggaattttct
      841 cccccaatcg gcgcggacga acgcgacgtt cctccagcgc ccccgccaac aacgcttttc
      901 acgacggagg aacccaaacg aagaatggct gaaggtcgac gctcgaacac ggatcctcaa
      961 cacgccgcga cgatcaaagt gatcggcatc ggcggaggcg gctgcaacgc cgtcaatcgc
     1021 atggtgcgcg ccgggctcgc cggcgtggat ttcttttcgg tgaactccga cgtgcaggcg
     1081 ctgcgcagtt cggccacgga gaacaccgtg cacatcggcg gcggtcagac gcgcgggcta
     1141 ggagccggcg cgaatccgac gctcggacga gaagccgccg aggaatcgcg cgaggacctc
     1201 gcgctgattc tagacggtgc ggatctcgtg tttctcaccg ccggcatggg cggcggaacc
     1261 ggcaccggcg cggctccggt gattgccgag atcgcccgcg aggcgggggc actgacgatt
     1321 gccgtcgtca ccaagccgtt tgcgttcgaa ggcaagaaac gcatgcaggt ggccgagcac
     1381 ggcatcgtcg agctggagtc gaaggtcgac acgctgatca ccatcccgaa cgagcggatc
     1441 ctgcaagtca tcgaaaagaa gacgccgctc aacgaagcgt tcgcctacgc cgacgacgtc
     1501 ctccgccagg gaatccaagg catcagcgac ctgatcacgc agcccggcct catcaatctc
     1561 gatttcgccg acgtcaagac gatcatgacc gatgcgggct cggcgatgat gggcatcggt
     1621 gaaggttccg gcgaacaccg cgccgccgac gccgcgcaga aagcgatcgc ctcgccgctg
     1681 ctcgaaacga cgatcgaggg cgcgcgcggc gtgatcttca acatcacggg cggtccggac
     1741 atggccatgt acgaggtgaa cgaggccgcc gaaatgatca gccgcgcagt cgatcccgat
     1801 gcccagatca tcttcggggc atcggtggat cccagcatga gcgggaaagt gcgggtcacc
     1861 gtgctggcag ccggcttcgg cgcgccgcgc gggtatcgcg acacccaaag cggctacgca
     1921 ttcgacaaga ccggcaagct cgaaacggtc gcaccggtga acatggacga catcgaagtt
     1981 ccggcgtttc tacgctatcg cggctaatcg ccggcggtca tggcgtactc cgcccgaata
     2041 ctgctcgatt cggtaagtcc ggcgggggtt aggctcac
//
LOCUS       DASUCJ010000612         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_908352, whole genome shotgun sequence.
ACCESSION   DASUCJ010000612 DASUCJ010000000
VERSION     DASUCJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908352"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFU90_07255"
     CDS             <1..959
                     /locus_tag="VFU90_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479615.1"
                     /translation="SDDAVLLKRIPEKVLVIGGGVIGLEFATVFARMGAAVLVVEMLP
                     QILSGTDLDIAKTLGRILKKQGVEIMLGTKVGKIEKDGKTLRATFSGEGTGGKDETRE
                     FDTVLVAVGRRPVTDNLNLAAAGLAADEKGFIAVDAQLRTKVPNVFAIGDVSGQPLLA
                     HRAMKQGVIAAEVIAGDKSAAFDPAAIPNCVYTDPEVATVGLSEEEAKAKGYEVRIGK
                     FPLAASGRARTMNESDGAIKLVGDAKSDLLLGMHIVAPQAESLIGEGVIALEMGATLE
                     DIGLSVHPHPTLTEGIMDAAEAAHGKAIHIVNPKPKTPAAAS"
     gene            964..1647
                     /gene="lipB"
                     /locus_tag="VFU90_07260"
     CDS             964..1647
                     /gene="lipB"
                     /locus_tag="VFU90_07260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5479616.1"
                     /translation="MAIAEAPVTSARLLDLGVRPYREVWALQHALHDAVRHGREPDTW
                     IVVEHPPTITLGRQAKREHVLFSSEELAARGIDLVEIERGGDVTYHGPGQLVVYPIRR
                     LERFREVVPLVRALEGAVIEACDAFGVPAQRWSEHAGVWVGGDQICAIGLAIRNMVSL
                     HGIALNVSTELSYDRLIVPCGLPGRGITSLSKEAGRPVGVEEAKHALLLALERTFDLT
                     FNSWPSTSI"
     gene            1626..>2356
                     /locus_tag="VFU90_07265"
     CDS             1626..>2356
                     /locus_tag="VFU90_07265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5479617.1"
                     /translation="MAIDVDLNALPHPRPPLHVRKPGWLRVSLPSGDDYERVKAKVDA
                     LALNTVCKEAACPNLAECWGAGTATIMILGDTCTRGCRFCNVKTGNPRGEVDWLEPVR
                     VAEAVRDLGWKYLVLTAVDRDDLPDGGALIFANTVREIHARVPGAKVEILSGDYRGDL
                     TALDIVLDARPDVFAHNLETVARLTPAVRDKRAGYAQSLKVLEHAKRRAPERYTKTSL
                     MLGLGETEPEIETAMDDARTAGVDI"
BASE COUNT          443 a          772 c          778 g          363 t
ORIGIN      
        1 actccgacga cgccgtcctc ctcaagcgca ttcccgagaa agtgctcgtg atcggcggcg
       61 gcgtcatcgg tttggaattc gcaaccgttt tcgcacgcat gggcgccgcc gtgctggtag
      121 tggagatgct gccgcagatt ctcagcggta ccgacctcga tatcgccaag acgctcggac
      181 gaattctgaa aaaacaaggc gtcgagatca tgctcggcac caaggtcggc aaaatcgaga
      241 aagacggaaa gacgctccgt gcgaccttca gcggggaagg caccggcggt aaagacgaaa
      301 cgcgcgagtt cgatacggtg ctggtcgccg tcggccggcg ccccgtcacc gacaacctca
      361 acctggcggc cgccggacta gcggccgacg aaaagggttt catcgcggtc gacgcccagc
      421 tgcgcacgaa ggtgccgaac gtcttcgcca tcggcgacgt tagcgggcag ccgctgctcg
      481 cgcaccgcgc gatgaaacag ggcgtgatcg ccgccgaagt aatcgccggc gacaaatcgg
      541 cggcgttcga tccggccgcc attcccaact gcgtctacac cgaccccgag gtcgccaccg
      601 tcggcttatc cgaggaagag gccaaagcca aaggctacga agtacggatc ggcaagttcc
      661 cgctggcagc cagcggacgc gctcgaacga tgaacgagtc cgacggtgcg atcaagctcg
      721 tcggcgatgc caagagcgat ctgctgctcg ggatgcacat cgtcgcaccg caggccgaat
      781 cgctgatcgg cgaaggcgtc atcgcgctgg aaatgggcgc gacgctcgaa gacatcggcc
      841 tgtcggtaca tccgcatcca acgctgaccg aaggcatcat ggatgccgcc gaagcggcgc
      901 acgggaaagc gattcacatc gtcaatccga aaccgaagac gccggccgcc gcgtcgtagg
      961 ggtgtggcga tagcggaggc gcccgttacg agcgcgcggc tgctggatct cggggtgcgg
     1021 ccctaccggg aagtgtgggc gttgcagcac gcgctgcacg atgcggttcg gcatgggcgc
     1081 gaaccggata cctggatcgt ggtcgaacat ccgccgacga tcacgctcgg ccgccaagcc
     1141 aagcgcgaac acgtgctctt ctcgagcgag gagctggccg cgcgcgggat cgacctcgtc
     1201 gagatcgagc gcggcggcga cgtgacctat cacgggcccg ggcagctcgt cgtctacccg
     1261 attcgccggc tggagcgctt tcgcgaggtg gtgccgctgg tacgcgcgct cgaaggtgcg
     1321 gtcatcgagg cttgcgacgc gttcggcgtg ccggcgcagc gctggagcga gcatgccggc
     1381 gtgtgggtcg gcggcgatca gatttgcgcg attgggctgg cgatccgcaa catggtttca
     1441 ctgcacggca tcgcgcttaa cgtgtcgacc gagctgagtt acgatcgcct gatcgtgccg
     1501 tgcggcttgc cgggccgcgg catcacctcg ctatctaagg aagccggacg gcccgtcggc
     1561 gtcgaagaag ccaagcacgc cctgctcctc gcgctcgagc gcaccttcga tctgaccttc
     1621 aattcatggc catcgacgtc gatctaaacg ccctgccgca tccccggccg ccgctgcacg
     1681 tgcgcaagcc cggctggctg cgcgtttcgc tgccctccgg cgacgactac gagcgggtca
     1741 aggctaaggt cgacgcgctg gcgctcaaca ccgtctgcaa ggaagcggcc tgccccaacc
     1801 tggcggagtg ctggggtgcg ggcacggcga cgatcatgat cttaggcgac acgtgtaccc
     1861 gcggctgccg cttctgcaac gtaaaaaccg gcaatccgcg cggcgaggtc gactggctgg
     1921 agcccgtccg ggtggccgaa gccgtccgcg atttgggctg gaaatatctg gtgctcaccg
     1981 ccgtcgaccg cgacgacctc cccgacggcg gcgcgctgat cttcgcgaac acggtgcgcg
     2041 aaattcacgc gcgcgttccc ggagcgaagg tcgagattct cagcggcgac tatcgcggtg
     2101 acctgacggc gctcgacatc gtcctggacg cgcgccccga cgtgttcgca cacaacctcg
     2161 aaacggtcgc ccgtctgacg cccgcggtcc gcgacaagcg cgccggctac gcgcaatcgc
     2221 tgaaagtgct cgagcatgca aagcgccgcg cgccggaacg ctacaccaaa acgtcgctga
     2281 tgctcggcct gggcgaaacg gaaccggaaa tcgaaaccgc aatggacgac gcacggacgg
     2341 ccggcgtcga catctt
//
LOCUS       DASUCJ010000613         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1168291, whole genome shotgun sequence.
ACCESSION   DASUCJ010000613 DASUCJ010000000
VERSION     DASUCJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168291"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1030
                     /locus_tag="VFU90_07270"
     CDS             <1..>1030
                     /locus_tag="VFU90_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU5479618.1"
                     /translation="AELRADGVQATAICLINAHQNPENERAVAAMLQARLPDLPIVAS
                     TEIVRERGEFERTSTTILNAYLMPTMQQYLSSLATALKERSVSVPLNIMGSNGGAMTF
                     EQASRFVVGTFLSGPVGGAIASMELGRTLDVGDFITFDMGGTSTDVLLVHRGQPRLSF
                     DNQIYAYPLRAPQLDIHTIGAGGGSIIRAQLDGTIEVGPQSAGAVPGPACYGRGGELA
                     TISDANLLLGRLPSEHDLSSNLRLSVPRAEDAMDRLARDISPDGGVDRVRLAAAAISL
                     AVSKMAGAVREISVHRGFDPREFVLLPFGGAGPLHAFLVANELGMQYVIVPPFPGHVS
                     ALGQMCAD"
BASE COUNT          174 a          349 c          321 g          186 t
ORIGIN      
        1 tcgccgaatt acgcgccgac ggcgtacagg caacagccat ctgcctaatc aacgcccacc
       61 agaatccgga aaacgagcgt gcggtggcgg cgatgctgca ggcccgcctg cccgatctgc
      121 cgatcgtcgc gtccacagag atcgtgcgcg agcgtggtga atttgagcgc acgtcgacca
      181 ctatcctcaa cgcgtacttg atgccgacga tgcagcagta cctgagctca ctcgctacgg
      241 cgctcaagga gcgctccgtt agcgtgccgc ttaacattat gggctcgaat ggcggcgcca
      301 tgaccttcga gcaggcatcg cggttcgtcg tcggaacctt tctatcgggg ccggtcggag
      361 gcgcaattgc gtcgatggaa ctcggccgca cgcttgacgt cggcgacttc atcacgttcg
      421 acatgggtgg cacgagcacg gacgtgctgc tcgtccatcg tggacagccg cgtctatcgt
      481 tcgacaacca gatctacgcg tatccacttc gcgcgccgca gctcgacatc catacgatcg
      541 gcgcgggcgg tggcagcatc atccgcgcac agcttgacgg caccatcgaa gtcggcccgc
      601 agagtgcggg tgcagtgccc ggtcctgcgt gttacggacg cggtggcgag ctcgcgacga
      661 tctcggacgc caacctgcta ctcgggcgtc tgccgtcgga acacgaccta tcgagcaatc
      721 tccgcctgtc cgtcccgcgc gccgaggacg ccatggaccg cctcgcccgg gacatatcgc
      781 ccgatggcgg cgtcgaccgc gttcgacttg ccgcggccgc gatcagcctc gccgtatcga
      841 agatggcggg cgcagtgcgc gagatctccg tccatcgcgg cttcgacccg cgggagttcg
      901 tgctgctgcc attcggcggc gccggacctc tacacgcatt cctggtcgcc aacgagctcg
      961 gaatgcagta tgtcatcgtc ccgccgttcc cagggcacgt gtcggcgctc ggtcagatgt
     1021 gcgccgactt
//
LOCUS       DASUCJ010000614         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1183372, whole genome shotgun sequence.
ACCESSION   DASUCJ010000614 DASUCJ010000000
VERSION     DASUCJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183372"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..927
                     /locus_tag="VFU90_07275"
     CDS             265..927
                     /locus_tag="VFU90_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5479619.1"
                     /translation="MQDQSTDMPTTPEPAPPAPAAVPPRAADTPARALELLREGNARF
                     VEGRGFTGSLTARRLELTAGQSPFAIVLGCSDSRVPLETIFDQIPGQLFVVRVAGNFL
                     SDDGIGSIEYGVAVLHAPEIVILGHGSCGAVTAARDYVRDGVAPPGFIRHLVRAVEPA
                     VIAVRNEPGDWLDNAIARNVRDNVAALTQRSEIVAAAVAAGEVGVTGAVYDLGTGRVN
                     FL"
     gene            complement(1032..1733)
                     /gene="rplA"
                     /locus_tag="VFU90_07280"
     CDS             complement(1032..1733)
                     /gene="rplA"
                     /locus_tag="VFU90_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5479620.1"
                     /translation="MPQHHGKRFKKLVAEYDRKQSFTPPEAIALVKKNANAKFNETVE
                     AHIRLGVDPKKSDQNVRGTVLLPHGTGRVVRVIAFAKGDNAKAATEAGADVVGDQDLI
                     DRVKTGFTEFDVAVATPDMMAQVGKELGRILAQKMPNPKAGTVTPNIGAAIRDIKAGK
                     VEFRLDKTGIIHTIVGKANFEEKQLVENVATLIDAIVRAKPAAAKGTYLRSVTLTSTM
                     GPGVKIDPNRVKATA"
BASE COUNT          265 a          606 c          556 g          345 t
ORIGIN      
        1 tagtcggtca agaagaggtt ctgcgcggcg gccagccggt cgcgcaactc ttggaccgcg
       61 gtttcttttc gtgcggttgg cattcgatgg tctttctaaa agtgccgaag gaacgaaaaa
      121 agcgctcctc gatccgaaag agggcgctcg acgggacaaa ctacgtcacg cggttgcgtc
      181 ctcggcaggt cttgcggatt aaggccgaaa tcggccgcct gcggtcttcg gagcctggag
      241 taggctacca caggtttggg gccggtgcaa gaccaatcga ccgacatgcc gacgactccc
      301 gaacccgcgc cgcccgcgcc cgccgccgtt cccccgcgcg ccgccgacac gccggcccgc
      361 gccctcgagc tgctgcgcga aggcaacgcc cgcttcgtcg aaggccgcgg cttcacgggc
      421 tcgctgacgg cccgccgtct cgagctcacc gccgggcaaa gcccgtttgc gatcgtgctc
      481 ggctgctccg attcgcgcgt cccgctcgaa acgatcttcg atcagattcc cgggcagctg
      541 ttcgtcgtgc gcgtcgccgg caatttcctc agcgacgacg ggatcggctc gatcgaatac
      601 ggcgtggccg tcttgcacgc gccggaaatc gtgattctcg gccacggctc gtgcggcgcg
      661 gtcaccgccg cgcgcgacta cgtccgcgac ggcgtcgcgc cgcccgggtt catccggcat
      721 ctcgtgcgcg ccgtagaacc ggcggtgatc gcggttcgca acgaacccgg agattggctc
      781 gacaacgcaa ttgcgcgtaa cgtgcgcgac aacgtcgccg cgctcaccca gcgctcggaa
      841 atcgtggccg ccgcggtcgc cgccggcgaa gtcggcgtga ccggcgccgt ttacgatctc
      901 ggcaccggcc gcgtcaactt tctctaaccc gctgtcatcc tgagcggagc gagcggagcg
      961 ctcgcaaccg tgtcatcctg agcggagcga gcgtcagcga gcgcagtcga aggacgaagg
     1021 acgaagcgcg tctaggcggt cgctttgacg cggttcgggt cgatcttcac gcccgggccc
     1081 atcgtgctgg tgagcgtcac gctgcgcagg taggtgccct tcgccgcggc cggcttggcg
     1141 cgcacgatcg cgtcgatcag cgtcgcaacg ttttccacca gctgtttttc ctcgaagttc
     1201 gccttgccga cgatcgtgtg gatgatcccc gttttgtcga gacggaactc caccttaccg
     1261 gctttgatgt cgcgaatcgc cgcgccgatg ttcggcgtca ccgtgccggc cttcgggttc
     1321 ggcattttct gcgcgagaat acggccgagc tctttaccga cttgcgccat catgtccggc
     1381 gtcgcgaccg ccacgtcgaa ctcggtgaag ccggtcttta cgcgatcgat cagatcctga
     1441 tcgccgacca cgtcggcccc ggcctcggtc gcggctttcg cgttgtcgcc tttggcgaac
     1501 gcgatcacgc gcaccacgcg gcccgtgccg tgcggcagca agaccgtgcc gcgcacgttc
     1561 tgatcgctct tcttcggatc gacgcccagc cgaatgtgcg cttcgaccgt ttcgttgaac
     1621 ttggcgttgg catttttctt taccagcgcg atcgcttcgg gcggcgtgaa gctctgcttg
     1681 cggtcgtatt cggcgaccag ctttttgaaa cgctttccgt gatgctgcgg cattacgctt
     1741 ccacctccac gcccatcgag cgcgcggtgc cc
//
LOCUS       DASUCJ010000615         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_526198, whole genome shotgun sequence.
ACCESSION   DASUCJ010000615 DASUCJ010000000
VERSION     DASUCJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526198"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFU90_07285"
     CDS             complement(<1..979)
                     /locus_tag="VFU90_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5479621.1"
                     /translation="MVVTTHLVLVLASYTLAFVLRFDGMIPSAYGRIFVVTLAPLVVF
                     RLGAFAFYRLYSGWWRYVGMLDMFALIKAIAISSALFTVLLVFTGQAYAFPRSVIVID
                     AVLTLFFIGGVRFALRALRENRRPAAGGPRLRRVLIIGAGDAGELLLREMHNNRRLGY
                     VPVGFVDDDVRKTGFHIHGVPVLGTTESLGTTLATNPADELIIAIPSASRDQIQNIVN
                     RCLATRLPFKILPAIAALSGRVQMSQVRPVRVEDLLGRDPVDLDSAIVRGAIAGRRVL
                     ITGAGGSIGAELARQIATFDPAALTLMERSENALFLIEQELHRKHPALDV"
     gene            complement(1062..>1395)
                     /locus_tag="VFU90_07290"
     CDS             complement(1062..>1395)
                     /locus_tag="VFU90_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5479622.1"
                     /translation="YYDRAFADVPEVRTPTIDAGNESIYNQYTIRADRRDGLQAHLKG
                     KGIGSAVYYPLPLHLQPCFRYLGYREGSCPESERAAREVLSMPVYPELTRAQQDEVIG
                     AVRSFYGH"
BASE COUNT          262 a          461 c          476 g          196 t
ORIGIN      
        1 ggacgtcgag cgcgggatgc ttccggtgca gctcctgctc gatgagaaag agcgcgtttt
       61 ccgagcgctc catgagcgtg agcgccgcgg ggtcgaaggt cgcgatttgc cgagcgagct
      121 cggcgccgat cgacccgccg gcgcccgtga tgagcacccg gcgccccgca atcgcgccgc
      181 gtacgatcgc ggagtcgagg tccacgggat cccggccgag caggtcttcg acacgcaccg
      241 gacgcacctg gctcatctgc acgcgtcccg agagcgccgc gatggccggc agaatcttga
      301 acggcaggcg cgtcgccaga cagcggttga cgatgttctg gatctggtcc cgcgacgccg
      361 acgggatcgc gatgatcaac tcgtccgccg gattggtcgc gagcgtcgtg cctaacgact
      421 cggtggtgcc gagcaccggc acgccgtgga tgtggaagcc ggtcttgcga acgtcgtcgt
      481 ccacgaagcc gacgggcacg tagcccaggc ggcggttgtt gtgcatttcg cgcagcagca
      541 gctcgcccgc gtcgccggcg ccaatgatga ggacgcggcg gagccggggg ccgccggccg
      601 ccggacgccg gttctcacgc aacgcgcgca gcgcgaaccg cacgccgccg atgaagaaca
      661 acgtgagcac cgcgtcgatc acgatcaccg agcgcgggaa cgcatacgcc tggcccgtga
      721 acacgagcag cacggtgaac aacgcgctgc tgatggcgat cgccttgatc agcgcgaaca
      781 tgtcgagcat gccgacgtac cgccaccacc cgctgtacaa ccggtagaag gcgaacgcgc
      841 ctaaccgaaa cacgacgagc ggcgccagcg tcacgacgaa gatgcggccg tacgccgacg
      901 gaatcatccc gtcgaagcgc agcacgaacg ccaacgtgta gctggcgagc acgagcacca
      961 aatgcgtcgt gacgaccatg agacgccggt accggaaggc cggcgtcgac agccatgcca
     1021 gtgcgcgctc cgtgaaggac ggaggcgcgg gttccgccgg ctcagtggcc atagaatgaa
     1081 cggacggcgc cgatcacctc atcctgttgc gcgcgcgtga gctcgggata caccggcatc
     1141 gagagcacct ctcgcgcagc gcgttccgat tccgggcacg agccctcgcg atacccgaga
     1201 taccggaagc acggctgcag gtgcagcggc agcggataat agacggccga gccgatgcct
     1261 ttgcccttga gatgtgcttg caagccgtcg cggcgatccg cgcgaatggt gtactggttg
     1321 tagatcgact cgttgccggc gtcgatcgtc ggcgtgcgca cctcgggcac gtcggcgaac
     1381 gcgcgatcat agtac
//
LOCUS       DASUCJ010000616         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1473851, whole genome shotgun sequence.
ACCESSION   DASUCJ010000616 DASUCJ010000000
VERSION     DASUCJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473851"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFU90_07295"
     CDS             complement(<1..1080)
                     /locus_tag="VFU90_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5479623.1"
                     /translation="MPAIQANENRVPAGTLSNGVLHLTLDARTGLWQPDGPSREVLVV
                     QAFGEPGRPLQAPGPLVRVPAGTIVDATVRNELPGKTLAMHGLQDRPANLDESFKLAY
                     GAQRTVRFRLNKAGTYYYWATTTGQPFSVREGADAQLNGAIVVDPPNYKFNPNNDRVF
                     VITDWDDVHKGHKMAFIYQQEMINGRAFPATERLSYERGTNVIWHLVNASYEDHPLHL
                     HGFFFDVLARGNGLTDAPYPAGQRDREVTELSTSGTTSTLAWTASRPGNWMLHCHLAY
                     HIMAHTPLNLPPNDKPSFERYEHSGKMGGMIVLLTVTPKPGDPPVTDPKAARNLKLIV
                     ERAPEDKPHAPAFAYVLEENGTATTG"
     gene            complement(1234..>1699)
                     /locus_tag="VFU90_07300"
     CDS             complement(1234..>1699)
                     /locus_tag="VFU90_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5479624.1"
                     /translation="KIATAEQAARALQPLAGHFAAWLFALGMVGTGLLAIPALVGSSA
                     YVIAEVLGFKRGLGQKPRRAKEFYGVLAGGIAVSILMGFLRVDPIPALFWSAVLNGVV
                     AVPLLAIIVLFCSNRGLMKEWASSPLARAWGWATVAVMALAAIGMFAFWGKS"
BASE COUNT          282 a          516 c          597 g          304 t
ORIGIN      
        1 tccggtggtg gccgttccgt tttcttcgag cacgtaggcg aacgcgggag cgtgcggctt
       61 gtcttcgggc gcccgctcca cgatcagctt gaggttgcgc gcggctttcg gatcggtgac
      121 gggcggatcg ccgggtttgg gcgtgaccgt cagcagtacg atcatgccgc ccatttttcc
      181 ggagtgttcg taccgttcga acgagggctt atcgttgggc ggcaagttca gcggcgtgtg
      241 ggccatgatg tggtacgcga ggtggcaatg gagcatccag ttgccagggc ggctggccgt
      301 ccacgcaagc gtgctggtcg taccggacgt gctgagctcg gtgacttcgc gatcgcgttg
      361 tccggcggga tagggcgcgt cggtcagccc gttgccgcgc gcgagcacgt cgaaaaagaa
      421 gccgtgcaga tgcagcggat gatcttcgta gctggcgttg acgaggtgcc aaatcacgtt
      481 ggtgccgcgt tcgtacgata gccgctcggt cgcggggaag gcgcggccgt tgatcatttc
      541 ctgctgatag ataaaggcca tcttgtggcc tttgtggacg tcgtcccaat cggtgatgac
      601 gaagacgcgg tcgttattcg ggttgaactt gtagttcggc gggtcgacga cgatcgcgcc
      661 gttgagctgc gcgtcggcgc cttcgcgaac gctgaaaggc tggccggtgg tcgtcgccca
      721 gtagtaatac gtgccggctt tgtttaggcg aaagcggacc gtgcgctgcg cgccgtacgc
      781 gagcttgaac gactcgtcga ggttcgcggg ccgatcttgc aggccgtgca tcgcaagcgt
      841 tttgccgggg agctcgttgc gcacggtggc gtcgacgatc gtgccggcgg gaacgcgcac
      901 gagcggtccc ggcgcctgca gcggccggcc gggttcgccg aacgcttgca cgacgagcac
      961 ttcgcggctg ggaccgtcgg gttgccacaa accggtgcgc gcgtcgagcg tgaggtgcag
     1021 cacgccgttg gacagcgtgc cggcgggaac gcggttctcg ttcgcctgaa tcgccggcat
     1081 cgcagccgac gctcggcttg gtggggcgat caagagggcc gcgcccaacg caaaaaagag
     1141 ggctgctcta ggtcggttca ttccgcaacg cctttccccc ggtccgaccg ctcagttcga
     1201 tgccaagcta gggagcgtcc tctcggcgac gcgctaggac ttgccccaga aagcgaacat
     1261 gccgatcgcg gcgagcgcca tgacggcgac ggtcgcccat ccccacgcgc gcgcgagcgg
     1321 cgaactcgcc cactctttca tcaggccgcg attgctgcag aacaagacga tgatcgcgag
     1381 cagcgggacc gcgacgacgc cgttgaggac cgccgaccag aacagcgccg ggatcggatc
     1441 gacgcgcaga aagcccatga gaatcgacac cgcgatgccg ccggccagca cgccgtagaa
     1501 ctctttggcg cggcgcggct tctgcccgag accgcgcttg aaccccaaga cttccgcgat
     1561 cacgtacgcg gacgagccga ccagcgcggg gattgcgagt agaccggtgc cgaccattcc
     1621 gagtgcaaac agccacgcag caaaatggcc ggccagcggc tgaagcgctc tcgcggcctg
     1681 ttcggcggtg gcgattttg
//
LOCUS       DASUCJ010000617         1993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1473856, whole genome shotgun sequence.
ACCESSION   DASUCJ010000617 DASUCJ010000000
VERSION     DASUCJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473856"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..922
                     /locus_tag="VFU90_07305"
     CDS             209..922
                     /locus_tag="VFU90_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479625.1"
                     /translation="MRIFAGAAILACLGASAPPGRLVYAFTYQSSQTNSAVHAYGVDK
                     MDPGGGGTFLFHNVNQHFIAPGANAPQTRTGTIAVDVVRQQSDGGLVVRVSETPAAAG
                     GEKPATCVTYGDTTVVCDPEHPLGPEANVLLRLLGKDFVDAGRLDAQRHWQIAGVGVP
                     GETDDYRIEGTAGGSIEILESGVIPANGTGKTTIDAKIDYDGVRALPTSVYRSTVEHS
                     RRGAVDETVSTQTTLKLEP"
     gene            complement(919..>1993)
                     /locus_tag="VFU90_07310"
     CDS             complement(919..>1993)
                     /locus_tag="VFU90_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479626.1"
                     /translation="VLANYPNVNQVPFDRDPSVSDGPFELVSWGHGDRLVFAANPHFF
                     KGLPGLKRVIVEIIADDNTAINQLRTHAIDYFFQPSIATYPALKDVPDARVVFVDMNA
                     YEGLEFNLSHPGVSDPVVRLAIAHAIDKPSFVTRLTHDQDKVATEDIPDWMWAFDPNV
                     KSYPYDVAAAKALLTRAGYDFDAGGMARRNGRPFHLLLATDSANVTHREESLLVQEAL
                     RAIGIDVEIKYFPQSMLYATAAMGGILQSGKFDLTLAPWYAGIDPDDSSQFLCATIAP
                     RGYNESRYCNPDMEAAQRSALDHYDQALRGVAYSRIQRLLARDNPYVFLWWQRQQEAV
                     SVDFKGFAPNPVTESWNAWQWRI"
BASE COUNT          364 a          610 c          692 g          327 t
ORIGIN      
        1 cgtccgctcg cgcgccgcgt cggcgagcgg cgcgcgcggc tcgcgtacga gcgcgcactg
       61 cgcggcgtgg aggcggtggt gaccggaaaa gcggggaacg acccggacga ctatcgcacg
      121 ctcgtcgaac ggatcagcct ggagctgtcg gggctgtcgg gcggctaacg cgcgtcacgg
      181 ccggtcgcgt gcgtgcgttc ttgcgtcgat gcgcattttc gcgggggcgg cgatccttgc
      241 gtgtctggga gcgagcgcgc ctcccggccg gctcgtgtac gccttcacgt accagagcag
      301 tcaaaccaac agcgcggtgc acgcctacgg cgtcgataag atggacccgg gcggcggcgg
      361 cacgttcttg tttcacaacg tcaaccagca cttcatcgcg cccggcgcga acgcgccgca
      421 aacgcgcacc ggcacgatcg cggtggacgt cgtccgccag cagtccgacg gcggattggt
      481 cgtacgggtg agcgagacgc cggcagccgc gggcggcgaa aaaccggcga cctgcgtgac
      541 ctacggcgac acgaccgtcg tgtgcgatcc ggagcatccg ctcggaccgg aagcgaacgt
      601 gctgttacgg ctgctcggaa aggatttcgt cgacgcggga cgcctcgacg cgcagcggca
      661 ctggcaaatc gccggtgtgg gcgtgccggg cgagaccgac gactaccgga tcgaaggcac
      721 cgccggcggg tcgatcgaga tcctcgagag cggcgtgatc cccgcaaacg gaaccggcaa
      781 aacgacgatc gatgcaaaga tcgactacga cggcgtgcgt gcgctgccga catcggtgta
      841 tcgctcgacc gtcgagcata gccggcgcgg tgcggtcgac gaaacggtct cgacgcaaac
      901 cacgctcaaa ctcgaaccct agatccgcca ctgccaggcg ttccacgatt cggtcaccgg
      961 gttgggcgcg aaccctttga agtcgacgct gacggcctct tgctggcgct gccaccagag
     1021 gaacacgtac ggattgtcgc gcgccagcag ccgctgaatg cgggaatacg cgacgccgcg
     1081 caatgcctga tcgtagtgat cgagcgccga acgctgcgcc gcctccatgt cgggattaca
     1141 gtagcgcgac tcgttgtaac cgcggggtgc gatggtggcg cacaggaact gcgaagaatc
     1201 gtccggatcg atgccggcat accacggcgc gagcgtcaaa tcgaattttc cgctttggag
     1261 gatgcccccc atcgccgcgg tggcgtagag catgctctgc ggaaagtact tgatttcgac
     1321 gtcgatcccg atggcacgca gcgcttcctg cacgagcagg ctctcctcgc gatgggtgac
     1381 gttggcgctg tcggtcgcga gcagcaagtg gaaaggacgg ccgttcctac gtgccatgcc
     1441 gccggcgtcg aaatcgtacc cggcgcgcgt cagcagcgcc ttcgcagcgg cgacgtcgta
     1501 gggataggac ttgacgttcg ggtcgaacgc ccacatccag tcgggaatgt cttcggtcgc
     1561 gactttgtcc tgatcgtgag tcagacgcgt tacgaaactc ggcttgtcga tcgcgtgcgc
     1621 gatcgcaagg cggacgaccg gatcggagac gccggggtgc gagaggttga actcgagacc
     1681 ttcgtaggcg ttcatgtcga cgaacacgac tcgggcgtcg ggaacgtcct tgagcgccgg
     1741 atacgtcgcg atcgagggct ggaagaaata gtcgatcgcg tgcgtgcgca gctggttgat
     1801 ggcggtgttg tcgtcggcga tgatttcaac gatcacgcgt ttcaagccgg ggagcccctt
     1861 gaagaagtgc gggttggcag cgaacacgag ccggtcgccg tggccccacg acacgagttc
     1921 gaacggaccg tcgctgacgg aggggtcacg atcgaacggc acctgattga cgtttggata
     1981 gttcgcgagc acg
//
LOCUS       DASUCJ010000618         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_220647, whole genome shotgun sequence.
ACCESSION   DASUCJ010000618 DASUCJ010000000
VERSION     DASUCJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220647"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      187..377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            598..>1543
                     /locus_tag="VFU90_07315"
     CDS             598..>1543
                     /locus_tag="VFU90_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5479627.1"
                     /translation="MGLIFRLTGAALALGLAAQDAHAQDNGAPLVLPTIDVGVTRIGN
                     GGITGASTSVITSEDIARSPRQTLTEIISEQAGVQIQHVGAGVNGARDVIDLRGFGAS
                     ATSNVLVLVNGRRFNDFDLQGFDYSSVPLNAIERIEVTRGNSGAVLYGDGAVGGVINI
                     VTKNGVGQPPSARVETAFGSFRTKEVRTSASGSSGPLSVALNTFGLTTDGYRENSKLH
                     QKGINGDIRYTTSEGSVYFNVLADDQKLGLPGGRTVDPSIGVNQLITDRTGAATPNDY
                     SNKQGQNYTLGVSRVLAPGLEAIVDGSYRRKTQQAELFF"
BASE COUNT          292 a          499 c          512 g          240 t
ORIGIN      
        1 tgccgcggtg aagacgcaca aggcgaagaa gcatgtcgcg cttcatcacc accgcaagca
       61 cgtgaagcac gtcaagcatg ccaagcgtca tgtgaagcat gccgtgaaga cggccggctg
      121 atcttctccg gctagcgagc aggcgccgcg tagcccgcgg cgcttgactc gtcgcgacct
      181 tccgcgccac attttcgccg tgctggttcc ccgggagacc ggggacgaaa gagggaacac
      241 ggaccatatc cgtggctgcc cccgcaactg tgagcggata gctcgcgtcc tgatggccac
      301 tgggaaaccg ggaaggccgg acgcaactga gcgacgaccc gcgagccagg agacctgcca
      361 gcacagtcac ccaaccggtg gacggggcgt ccatacggag cggtctttcg cagcggtgac
      421 ggcaatgccg gtgcgaaggc cgtgcggcgg gtgaattgtc cctccaatcg tcttcgaaac
      481 gacagcgtcg cgcggcaggg tccgtcgggc gtttcgtttc cttcgcatcg ggtccgctct
      541 gacccgctgt gcgcgacgca tggaatgcgt tgcgcgcgaa cgaaggaacg ttgcgacatg
      601 ggactcatat ttcgtctgac cggcgcggca ctcgcgctcg gtcttgccgc gcaggacgcg
      661 cacgcgcaag acaatggcgc gccgcttgtg ctgccgacca tcgatgtcgg tgtcacgcgc
      721 atcggcaatg gcggcatcac cggcgcgtcg acatcggtaa tcacgtcgga agacatcgcg
      781 cgctcgccgc ggcagacgct caccgagatc atcagcgagc aggccggcgt gcagattcag
      841 cacgtcggcg ccggcgttaa cggcgcgcgc gatgtcatcg acctgcgcgg ctttggcgcc
      901 agcgcgacat cgaacgtgct cgtcctggtg aacggccggc gcttcaacga tttcgacctg
      961 caaggcttcg actattcgtc ggtcccgctc aatgcgatcg agcgcatcga ggtgacgcgc
     1021 ggcaacagcg gcgccgtgct ctacggcgac ggcgcggtcg gcggggtgat caacatcgtc
     1081 acaaaaaacg gcgtcggcca gccgccgtcg gcgcgcgtcg agaccgcctt cggctcgttc
     1141 cggaccaagg aagtgcggac ctcggccagc ggctcgtccg ggccgctgtc ggtggcgctg
     1201 aatacgttcg gcctgacgac cgacggctat cgcgagaaca gcaagctgca ccagaaaggc
     1261 atcaacggcg atatccgcta cacgacgagc gagggcagcg tctatttcaa cgtcctcgcc
     1321 gacgatcaga agctcggact tccgggtggg cggacggtcg atccgagcat cggcgtcaac
     1381 cagctgatca ccgaccgcac cggcgcggcg acgccgaacg attacagcaa caagcagggc
     1441 cagaattaca cgctcggcgt cagccgggtg ctggcaccgg ggctcgaggc gatcgtcgat
     1501 ggcagttatc gccgcaagac gcagcaggcg gagctgttct tca
//
LOCUS       DASUCJ010000619         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_648694, whole genome shotgun sequence.
ACCESSION   DASUCJ010000619 DASUCJ010000000
VERSION     DASUCJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648694"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFU90_07320"
     CDS             complement(<1..626)
                     /locus_tag="VFU90_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479628.1"
                     /translation="MNLRQDRGYAAESRELDAGILGVIRRWHDAGESPSDAAFDDLAL
                     RLCAHQLRFNRPYARYCATLGVTPSSLPRSWADIPPAPAAAFKDAELATFDPAVAALS
                     FETSGTTAGRAGRHFMESAALYEAALLAAFDRFVLPDRAALQYFNLVPDPRERPASSL
                     GYMMGRVSEQRGAGETGWYVHGDRLSYGELLRDLREAIAAERPVCIAAT"
     gene            complement(623..1099)
                     /locus_tag="VFU90_07325"
     CDS             complement(623..1099)
                     /locus_tag="VFU90_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5479629.1"
                     /translation="MILNTPAQRRALLDRVSTIAVVGASANPLRPSYTVFSYLRTQTP
                     YVVAPINPTIDAIDGVPAFPSLAAFAARYGAPDLVDVFRRPEQLVGVVDEAIAAGARA
                     IWFQYGVVNDAAIALADRAGMTVVVDRCIKVEHARFSGGLSAGGLNSGLITSRRRP"
     gene            complement(1096..>1315)
                     /locus_tag="VFU90_07330"
     CDS             complement(1096..>1315)
                     /locus_tag="VFU90_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU5479630.1"
                     /translation="LWSHLANVGDAKSLVIHPASTTHSQLSDAELAKAGVPPESVRLS
                     AGLEDVDDLIWDVDRALRAAVPAAATVS"
BASE COUNT          242 a          419 c          472 g          182 t
ORIGIN      
        1 gtggcggcga tgcaaaccgg acgctccgct gcgatcgcct cccgcaaatc gcggagcagc
       61 tcgccgtacg aaaggcggtc gccgtgcacg taccagcccg tctcgcccgc gccgcgctgc
      121 tccgaaaccc ggcccatcat gtatccgagc gacgacgcgg gacgttcgcg cggatccgga
      181 acgaggttga agtactgcag tgcggctcgg tcggggagca cgaagcggtc gaatgccgcc
      241 agcaaagcgg cctcgtagag cgcggcgctt tccatgaagt gccggccggc gcggccggcc
      301 gtcgtaccgc tggtttcgaa ggaaagcgcg gcgacggcag gatcgaacgt ggcgagttcg
      361 gcgtctttga aagcggccgc cggggcgggc gggatatcgg cccacgagcg gggcagcgac
      421 gacggcgtga cgccgagcgt cgcgcaatag cgcgcgtacg ggcggttgaa gcgcagttga
      481 tgcgcgcaca gacgcaaagc taaatcgtcg aacgcggcgt cgctcggcga ttcgccggcg
      541 tcgtgccagc gccggatgac gcccaaaatc ccggcatcga gctcccgcga ctcggcggcg
      601 tagccgcggt cttgccggag gttcacggtc gccggcggga ggtgatcaat ccgctattga
      661 gaccgcctgc agagagaccg ccggagaaac gcgcgtgctc caccttgatg cagcgatcga
      721 cgacgaccgt cattccggcg cggtccgcaa gcgcgatggc ggcatcgttg acgacgccgt
      781 attgaaacca gatcgcccgc gcgccggcgg cgatcgcttc gtcgactacg ccgaccagct
      841 gctccggccg gcgaaaaacg tcgacgagat ccggcgcacc gtagcgggcg gcgaaggccg
      901 caagcgacgg gaaggcgggg acgccgtcga tcgcgtcgat agtcggattg atcggcgcca
      961 ccacgtaggg cgtctgggtg cggagatacg aaaataccgt ataactggga cgcagcgggt
     1021 tggccgacgc cccgacgacc gcgatcgtcg aaacgcggtc gaggagcgcg cgacgttgcg
     1081 ccggcgtatt gaggatcacg aaaccgtggc tgccgcaggc accgccgcgc gcagcgcgcg
     1141 atcgacgtcc cagatcaaat cgtcgacgtc ttcgagaccg gccgaaaggc gcaccgactc
     1201 cggcggaacg ccggctttgg cgagctctgc gtcggacagc tgcgaatggg tggtagatgc
     1261 gggatggatt acaaggcttt tggcgtcgcc gacgttggcc aagtgactcc acagc
//
LOCUS       DASUCJ010000620         6541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1137807, whole genome shotgun sequence.
ACCESSION   DASUCJ010000620 DASUCJ010000000
VERSION     DASUCJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137807"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFU90_07335"
     CDS             <1..400
                     /locus_tag="VFU90_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479631.1"
                     /translation="GGIVHVNARLPERTTVPMQHIAFGDPATIYPPEKAAEVARRLEF
                     YETVFNLESGDDVRARAAEAYAKFLRKIHAQDTTLGEHRNVKPPPRRSGEEPPPTQAT
                     QVDKVVDVMMLELEEMEHRRQEAIKRQKER"
     gene            413..1216
                     /locus_tag="VFU90_07340"
     CDS             413..1216
                     /locus_tag="VFU90_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5479632.1"
                     /translation="MEWPAGLAPYVEFAKKAFAREATYRMEVLTEIGSLVLRVYILRS
                     LWTALYAQNVAPVNLPLHGMITYATVAMLMSLILEVDGTRLIREKVREGTIATDLMKP
                     ISLPLYFFSDGIGQTLLHAVLVVPSLLFALLLVRIDVPNPATFAAFAVAFLIGYCVNF
                     FLNFLMNSIAFWTLETFAVQLIVRWGSDLLSGQIIPLTFFPGILGRIVFGLPFAAIYS
                     TPLLIYVGIIPPSRWAVSIAVQLVWLLLFATLSTVVWRAASQKVVIQGG"
     gene            complement(1229..2629)
                     /locus_tag="VFU90_07345"
     CDS             complement(1229..2629)
                     /locus_tag="VFU90_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479633.1"
                     /translation="MRSDRFSLKRILAVAAAAASLGACAGGGNETAGGLPSAPNAGSF
                     RVDDGPMNLLRQKIKHVVIIVQENRSFDNLFQGFPGADTQSYGYTSGGQQVALQSVGL
                     ETSWDIVHDLSDYLNACDGSGSIPGTNCKMDGFDKEYAGCGHRYQPPCPIQFPEYAFV
                     PANETKPYFAMGEQYVVADRMFTSNLDGSSFISHQYIIGAQASSAVDYPSGAWGCDGG
                     PSDMVHTITQQRQYGPQESACFDNQTLGDELDKAGVSWHYYATAQYGQDFSFIWSAYQ
                     AIKHIYNGPDWSADVISPQTQIINDAQNGKLPAVSWVTPTCANSDHAGCGSNTGPAWV
                     ASVVNAIGQSQYWNSTAIFLMWDDYGGWYDHVSPPYEDYDGLGIRVPLVIISPFAKKA
                     FVSHVQYEHGSILRFVEDVFGLPRLSASDTRANSPAKDCFNFARLPRKFHVIPSSHDA
                     QFFLHQPPDPRPVDTQ"
     gene            complement(2645..4036)
                     /locus_tag="VFU90_07350"
     CDS             complement(2645..4036)
                     /locus_tag="VFU90_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479634.1"
                     /translation="MRSNLSGIGRILAVAVSASMLAACAGGTGSPGGFLAPSTAQAVR
                     SDDGPLNLMRQKIKHVVIIVQENRSFDNLFQGFPGADTQSYGYTTGGQQVTLQPVGLE
                     TSWDIDHSLGSYLAACDGSGSLPGTNCKMDGFNNEWAGCGHRSQPPCPNSNPQYSYVP
                     QSETKPYFAMGEQYVVADRMFASNLDGSSFISHQYIIAGQAASAVNYPSSYWGCDGGP
                     SDTVYTITQQRQYGNQEQACFDEQTLGDELDKAGVSWHFYTSTVNGDGGIWSAYQAIN
                     HIRYGPDWNSDIITPQSQIIQDAQNGNLPAVSWVTPTCAHSDHAGCGSNTGPAWVASV
                     VNAIGQSQYWDSTAIFLMWDDYGGWYDHVPPPLEDYDGLGIRIPLVMISPYAKKAFVS
                     HVQYEHGSILRVVEDVFGLPRLTASDTRANSPAKDCFDFARLPRKFHVIPSNLNAQYF
                     LHERPDLRPPDNE"
     gene            complement(4241..5101)
                     /locus_tag="VFU90_07355"
     CDS             complement(4241..5101)
                     /locus_tag="VFU90_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5479635.1"
                     /translation="MIRFIAVLAFAAPLFASASAQTARPVSVLYAGSLVTPMEGPIKA
                     SLAASGIDFQGEGGGSKVLANLIASGAKNPDVFVSVDPALVTGLGARVSYAATFASTS
                     LGIGWSSQSRYAALFASVAAGKTPLLAALSTRGLAIGRTDPKLDPKGAYTIEAMTLLA
                     GKDGERKILGDDENQAQTFPEEDLLARIETGQADAGFFYKTETVARGLKFFPLPGAAS
                     LSDRITYTLALMKGAPHPQAAAAFRDFLLRGDGKRILERAGLTYLAAPKALVGGPLSA
                     VRQDDGKIGR"
     gene            complement(5098..6009)
                     /locus_tag="VFU90_07360"
     CDS             complement(5098..6009)
                     /locus_tag="VFU90_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479636.1"
                     /translation="MRFGAGMELPVRAPFRLDLTVDALRRLATNAVDVVGPEGTFYRA
                     VRDARGSALLAIRSAGPRSVEVRATGRDARRWLPLVERMLGTETDLRDWYERSARIDW
                     LRPLAATLRGVKPPRYPSLWEACAHAVVFAQISIHAASAIMRRLVERLAEPVTAGGIR
                     AWIFPEPARWLAAPDAALREAGLSQNKIRNVRSIATALSDGTLSERALNLLPTPAAAE
                     ALVGVRGIGPWSASVVLLRGLGRLDTFPLRDSGVARSVALIAGTSVDLDAVLDILGPT
                     RGMLYFHLLLGRLHHAPPTAGGKDRTG"
     gene            6015..>6541
                     /locus_tag="VFU90_07365"
     CDS             6015..>6541
                     /locus_tag="VFU90_07365"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5479637.1"
                     /translation="MLAAYRQYWRVNFLTMVEYRANFVMWFFFTIMYHGVALAALYVT
                     MRQFPSMNGWDFRQMFFLYALWMTGHELHNSLFFTIVSVPDYIREGRFDRFLVRPLDT
                     LFQVLTVPQQIVPDGLLLAIATLVAATVAAGVRIDWIFVIFVPLVAIGGALIDFGISL
                     VVATVSFWFIRVDTLR"
BASE COUNT         1113 a         2106 c         2088 g         1234 t
ORIGIN      
        1 gggcggaatc gtccacgtga acgcgcggct gccggagcgc acgacggttc cgatgcagca
       61 cattgcgttc ggcgaccccg ccacgatcta tcctcccgag aaggccgccg aagtggcgcg
      121 gcgattggag ttctacgaga cggtcttcaa cttggaatcc ggcgacgacg tccgcgcgcg
      181 ggcggccgaa gcctacgcga agttcctgcg caagattcac gcgcaggaca cgacgctggg
      241 cgagcatcgc aacgtcaagc cgccgccgcg gcgttccggc gaagagccgc cgccgacgca
      301 ggcgacgcaa gtcgacaaag tggtcgacgt gatgatgctc gagctcgaag agatggagca
      361 tcggcggcag gaagcgatca aacgtcagaa agagcgttag gaaaccgcac tggtggaatg
      421 gcccgccggg ctagcgccct acgtggagtt cgccaagaag gcgttcgcgc gcgaggccac
      481 ctaccgtatg gaggtcctca ccgagatcgg ctcgctggtg ctgcgcgtct acatcttgcg
      541 ctcgctgtgg accgcgcttt acgcccaaaa cgtcgctccg gtcaaccttc ccctgcacgg
      601 catgatcacg tacgcgacgg tcgcgatgct gatgtcgctg atcttggagg tcgacggaac
      661 gcggctgatt cgcgagaagg tccgcgaggg cacgatcgca accgacttga tgaagccgat
      721 tagtttgccg ctttatttct tcagcgacgg catcggtcaa acgcttctcc acgccgtgct
      781 ggtcgttccg tcgctcctgt tcgcgctgct gctggtgcgt atcgacgtgc cgaatccggc
      841 cacgttcgcc gcgttcgccg tcgctttcct gatcggctac tgcgtgaact tcttcttgaa
      901 tttcctgatg aactcgatcg ccttttggac gctcgaaacg ttcgccgtcc agctgatcgt
      961 ccgctgggga tccgatctgc tgtcgggcca aatcatcccg ctgacgttct tccccggcat
     1021 cctcggacgg atcgtcttcg gattgccgtt cgccgcaatc tactccacgc cgctgctaat
     1081 ttacgtcggg atcattccgc ccagccgctg ggccgtcagc atcgcggttc agctcgtctg
     1141 gctgctgctg ttcgcgaccc tgtcgacggt cgtctggcgc gccgcatcgc agaaggtcgt
     1201 catccaggga ggctgaaccg cgccgccgtc actgcgtatc gaccggccgc ggatcgggcg
     1261 gctggtgcag gaagaattgc gcgtcgtggc tcgacgggat tacgtggaac ttgcgcggca
     1321 ggcgcgcgaa gttgaagcaa tccttcgccg gcgagttggc gcgggtatcg ctcgcgctca
     1381 gccgcggcaa accgaacacg tcctcgacga accgcaagat gctgccgtgc tcgtactgca
     1441 cgtgcgagac gaacgctttc ttggcaaacg gcgagatgat gaccagcgga acgcggatgc
     1501 cgaggccgtc gtagtcctcg tacggcggcg aaacgtggtc gtaccatccg ccgtagtcgt
     1561 cccacatcag gaagatggcg gtcgagttcc agtactggct ctggccgatc gcgttgacga
     1621 ccgaggccac ccaggccggg cccgtattcg agccgcagcc ggcgtggtcg gagttcgcgc
     1681 acgtcggcgt cacccagctc accgccggga gcttgccgtt ctgggcgtcg ttgatgatct
     1741 gggtttgcgg cgagatgacg tcggcgctcc aatccggtcc gttgtagatg tgcttgatcg
     1801 cctggtaggc gctccagatg aagctgaaat cctggccgta ctgggcggtc gcatagtagt
     1861 gccacgaaac gccggccttg tcgagctcgt cgccgagcgt ctgattatcg aagcaggccg
     1921 attcttgcgg accgtattgg cgctgctgcg tgatcgtgtg caccatgtcg ctcgggccgc
     1981 cgtcgcagcc ccacgcgccg ctcggatagt cgaccgcgga gctggcctgc gcgccgatga
     2041 tgtactgatg cgagatgaag ctgctgccgt cgaggttcga ggtgaacatc cggtcggcga
     2101 cgacgtactg ctcgcccatc gcgaaatacg gtttcgtttc gttcgcggga acgaacgcgt
     2161 actccggaaa ctggatcggg cacggcggct ggtaacggtg tccgcagccg gcgtactcct
     2221 tgtcgaagcc gtccatcttg cagttcgtgc ccggaatgct cccggagccg tcgcaggcat
     2281 tcaggtagtc gctcaaatcg tgcacgatgt cccagctcgt ttcgagcccg accgactgca
     2341 gcgccacctg ctgcccgccg ctggtataac cgtacgactg ggtgtcggca cccggaaatc
     2401 cctggaacag attgtcgaag ctgcggttct cttgaacgat gatgacgacg tgcttgatct
     2461 tctggcgtaa gaggttcatc ggcccgtcgt cgacgcggaa gctgccggcg ttcggcgcgg
     2521 acggcaggcc gcccgccgtt tcgtttccac cgcccgcaca agcgccgagc gatgctgccg
     2581 ccgcggcgac ggccaggatt cgcttgagac tgaaacgatc gcttcgcata tcgctggctc
     2641 ctcgctattc gttatcgggc ggccgcagat ccggacgctc gtgcaggaag tactgcgcgt
     2701 tgaggttcga cggaatcacg tggaacttgc ggggcaagcg cgcgaagtcg aagcagtctt
     2761 tcgcgggcga gttggcgcgc gtatcgctcg cggtcaggcg cggaaggccg aacacgtcct
     2821 cgacgacccg caagatgctg ccgtgctcgt attggacgtg cgagacgaac gccttcttgg
     2881 catacggcga gatcatcacc agcggtatcc ggatgccgag accgtcgtag tcttcgagcg
     2941 gcggcggaac gtgatcgtac caaccgccgt agtcgtccca catcaggaag atcgcggttg
     3001 agtcccagta ctggctttgc ccgattgcgt tgaccaccga cgccacccac gcgggccccg
     3061 tgttggagcc gcagccggcg tgatcggagt gcgcgcaggt cggcgttacc cagctcaccg
     3121 ccggcaggtt gccgttttgg gcgtcctgga tgatctgcga ctgcggcgtg atgatgtcgc
     3181 tattccagtc cggtccgtag cggatgtggt tgatggcttg atacgcgctc caaatgccgc
     3241 cgtcgccgtt gaccgttgag gtatagaagt gccacgaaac cccggccttg tcgagctcgt
     3301 cgcccagcgt ctgttcgtcg aagcaggctt gttcttgatt cccgtactgg cgctgctgcg
     3361 tgatggtgta gaccgtgtcg gacggcccgc cgtcgcagcc ccagtagctg ctgggatagt
     3421 tcacggccga agcggcttgc ccggcgatga tgtactgatg cgaaatgaag ctgctgccgt
     3481 cgaggttcga ggcgaacatc cggtcggcca cgacgtactg ctcgcccatc gcaaaatacg
     3541 gcttcgtctc gctttgggga acgtagctgt actgcggatt ggagttcggg caaggcggct
     3601 gcgaacgatg cccgcatccg gcccactcgt tgttgaagcc gtccatcttg cagttggtcc
     3661 ccggcaggct cccggagccg tcgcacgcgg cgaggtagga gccgaggcta tgatcgatgt
     3721 cccacgacgt ttcgagccca accggctgca gcgtcacttg ctgaccgccg gtcgtgtagc
     3781 cgtacgactg cgtgtcggcg cccggaaagc cttggaagag attgtcgaag ctgcgatttt
     3841 cttgaacgat gatgacgacg tgtttgatct tctggcgcat cagattcaac ggcccgtcgt
     3901 cgctgcgcac ggcttgcgcc gtgctcggtg ccagaaaccc tcccggcgag ccggtgccgc
     3961 cggcgcaggc agcgagcatc gacgccgaca ccgcgaccgc gagaattcgg ccgatacccg
     4021 aaaggttgct tcgcatgctg tctcctaaaa aaatgacgtc agttataaaa tgtcatccgg
     4081 ccggttggct aaaatcgccg accggctacc gtcgcgttcg tcgtgggcgt aagaaaaata
     4141 acggcccatg gctctccctc gattgccgac gaactcccgc acccgcgacg aaaggcgatt
     4201 ctatcgcaat tagagagacg cccagctact aggaaaagcg ttacctcccg attttgccat
     4261 catcctgccg gaccgccgaa agcggcccgc ccaccagcgc tttcggcgcg gcgagatacg
     4321 tgaggccggc tcgctcgagg atccgctttc cgtcgccccg cagcaaaaaa tcgcggaatg
     4381 cggccgcggc ctgcggatgc ggcgcgccct tcatcaatgc gagcgtgtac gtgatgcgat
     4441 cggagagcga cgccgcgccg ggtagcggga aaaacttcaa acctcgcgct accgtttccg
     4501 ttttgtaaaa gaagccggcg tcggcttgtc ccgtctcgat gcgcgccagc agatcttcct
     4561 cgggaaaggt ctgcgcttga ttctcgtcgt cgccgaggat ctttcgttcg ccgtccttac
     4621 ccgcaagcag cgtcatcgct tcgatcgtgt acgcgccctt cggatcgagt ttcggatcgg
     4681 tgcgcccgat cgccagcccg cgggttgaca gtgccgcgag cagcggcgtc tttcccgcgg
     4741 cgaccgaagc gaacagcgcg gcgtaccgcg actggctcga ccagccgatc cccaagctcg
     4801 tgctcgcaaa cgtagcggcg tacgagacgc gcgcgcccag gcccgtaacc agcgccgggt
     4861 cgacgctgac gaagacgtcg gggttcttgg cacccgacgc gatcaggttg gcgagcacct
     4921 tgctgccgcc gccctcaccc tgaaagtcga tgccggacgc cgccagcgac gctttgatgg
     4981 gaccttccat cggcgtgacg agcgaccccg cgtagagtac cgaaaccggt cgcgcggttt
     5041 gtgccgaggc cgaggcgaag agcggcgccg cgaacgccag cactgcgata aagcgaatca
     5101 accggtcctg tctttcccgc ccgccgtagg cggcgcgtga tgcaggcgtc cgagcagcaa
     5161 gtgaaagtac agcatccccc gcgtcggacc gagaatatcg agtacggcat cgagatcgac
     5221 gctcgtgccg gcgatcagcg cgacgctgcg cgccacgccg gaatcgcgca gcggaaacgt
     5281 atccagccgc cccaagccgc gcagcagcac caccgaagcg ctccacgggc cgatcccgcg
     5341 aacgccgacg agtgcttccg cagccgccgg agtaggcagc aggttcagcg cgcgctccga
     5401 caacgttccg tcgctcaagg cggtcgcgat cgaacgcacg tttcgaattt tgttttgcga
     5461 gagtccggcc tcacgcagcg ccgcgtcggg cgcggccagc cagcgcgccg gctccggaaa
     5521 gatccacgcg cgtatcccgc cggcggtcac cggttcggcg agccgctcca cgaggcggcg
     5581 catgatcgcc gaggcggcgt gtatcgaaat ttgtgcgaag acgacggcgt gcgcgcacgc
     5641 ttcccagagc gacggatagc ggggcggttt cacgccgcgc agcgtcgcgg cgagcggtcg
     5701 cagccagtcg atgcgggcgc tccgctcgta ccagtcgcgc aaatcggtct ccgttccgag
     5761 catgcgctcg accagcggca gccagcgccg cgcatcccgg ccggtcgccc gcacttcgac
     5821 cgaacgtgga ccggccgagc ggatcgccag cagcgcgctt ccgcgcgcgt cgcgtaccgc
     5881 acggtagaag gttccttctg gtccgaccac gtcgaccgca ttggtcgcca gccggcgcag
     5941 cgcgtcgacc gtgaggtcga gccggaacgg cgcgcggacg gggagctcca tcccggcacc
     6001 gaaacgcatc accgatgctc gcggcctacc ggcagtactg gcgcgtcaac ttcctcacga
     6061 tggtcgagta ccgcgcgaac ttcgtcatgt ggtttttctt taccatcatg tatcacggcg
     6121 tcgcgctggc cgcgctgtac gtgacgatgc gccagttccc atccatgaac ggctgggact
     6181 tccggcagat gttcttcttg tacgcccttt ggatgacggg ccacgagctg cacaactccc
     6241 tctttttcac catcgtcagc gtgccggatt acattcgcga gggtcgcttc gaccgctttc
     6301 tggtacgccc gctcgatacg ctctttcaag tgctgaccgt tccacagcaa atcgttcccg
     6361 acggtttgct gctggcgatc gccaccctgg tggccgcaac ggtcgcggcg ggcgtgcgga
     6421 tcgactggat cttcgtcatc ttcgtgccgc tggtggccat cggcggagcg ctgatcgatt
     6481 tcgggatctc gctggtggtc gcgaccgttt cgttttggtt catccgggta gatacgctgc
     6541 g
//
LOCUS       DASUCJ010000621         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_449919, whole genome shotgun sequence.
ACCESSION   DASUCJ010000621 DASUCJ010000000
VERSION     DASUCJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449919"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1368)
                     /locus_tag="VFU90_07370"
     CDS             complement(76..1368)
                     /locus_tag="VFU90_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5479638.1"
                     /translation="MRASHAWRPYVALAFVASCTVAAGCSSSGTVSQPPTPNPIGGAA
                     CLTTVPSPLPSPVASAFAEGISKVQGVPGLAISLVYKNAVYQYYSGYADVRTGRCMTA
                     GSIVELGSLTKTFTAALLALAYANGSLKGGDNPARFIKTLGHGNDVGPGCSPPPAPTP
                     VPPSAYGVMATMTLTMLATHTSGLPDVPPKEGALSERPCYSPNQLINFVETYPSPLPA
                     PTPPHPWLYSNIGFGTLGYVLQGINKKLWFEQVTQSLLLPLNMRRTYDVYDVPAKNYA
                     QGYDCTGTLAVQHWPIDAWPAAGTLRSTLPDMTRYLSAAMQRAGTPATIAKAMALAET
                     PVSGAYTPQGAKQGMAWATTATVPPVIYKDGATDGFNSFIGIVAARRGKPSTGIVVLT
                     NRAPVKQSPAPGPTCAPFVGSPADQIGFAVVRTLLTRR"
     gene            complement(1513..2304)
                     /locus_tag="VFU90_07375"
     CDS             complement(1513..2304)
                     /locus_tag="VFU90_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479639.1"
                     /translation="MNYLIFVANPWWLVVVLTIVLGLAIEVPFRAGLLLKVRIPGKMD
                     ALTAVQAGLLTLASFVLGLSFSQAEGRFDARRALVIREANAIGTTWLRADQLDEGQRR
                     RFRRILVDYTSTRLQAYQLPAASPGVSYRSDLDRSNRDQDAMWAIVSPALRAHPGDLG
                     LGLLMQTLNDTIDVSAEQLQALTSHIPTAVIELSLALYTLGALSLGLRFAADGVRPAA
                     LSAVYVVASVIVVTMMIDYDRPQTGFVTVNLSPLKVQLETMQASK"
     gene            complement(2382..2810)
                     /locus_tag="VFU90_07380"
     CDS             complement(2382..2810)
                     /locus_tag="VFU90_07380"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEU5479640.1"
                     /translation="MYDNRVTWKCPRCSRTFRRANQRHACGTGDDASLLANRPPELAE
                     LYRKLEAAVRSYGGVEIVARDRYALFRTTRIFADLTVMRDALRVVIHLARRVKKPCFV
                     KVGASGKRVSHVALVRTGDDLRTILPFLREAFDLAQSENT"
     gene            2870..3508
                     /locus_tag="VFU90_07385"
     CDS             2870..3508
                     /locus_tag="VFU90_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU5479641.1"
                     /translation="MSAQLFEAFYSFVTLLAMVNPIEAAAAFTTLTAGRSPQEQARIA
                     LRATVVAAAILLGFSYAGEALLNAMGISLAAFKIAGGLLLLKVGFDMVFAEKSATGGA
                     GGPAGPPASDPSVFPLAIPLISGPGALTAGVTLVSRAHENRVLADLAFVAIALVVFAI
                     TYLAMRGSERITKLLGPIGVDAAGRLVGIIVTAIAVQLVVNGAGSLVHYVVR"
     gene            complement(3505..>4115)
                     /locus_tag="VFU90_07390"
     CDS             complement(3505..>4115)
                     /locus_tag="VFU90_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479642.1"
                     /translation="IVRLLRNSPDFAGTAGLADALLHPRDRAYSVDQVMAFLERAGLR
                     FVRWIRQAPYLPECGAVAATPHRALLDRLSPRERFAAMELFRGTMVRHNFIAYRDDAG
                     VPDTPNFEGEAWLDYVPVRLPDTVSVRDRLPPGSTAVLINRNHTYTDLYLPVDKLREQ
                     LVKGIDGARTIGEICRPLASRDLAREFFLQLWQWDQVVFDAS"
BASE COUNT          708 a         1296 c         1402 g          709 t
ORIGIN      
        1 tgaaatttac cctccgcccg ggcaggttcg taagcaacgg cgcgctcgcc ttcgtaaggc
       61 gaagcctgcc aaagctcagc gccgagtgag cagcgtacga acgacggcga aacctatttg
      121 atcggccggc gagccgacga acggcgcaca ggtcggcccg ggcgcgggcg actgctttac
      181 gggagcgcgg ttcgtcaaca ccacgattcc ggtcgacggc ttcccccggc gagcggcgac
      241 gatgccgatg aagctgttga agccatcggt cgcgccgtct ttatagatga ccggcgggac
      301 cgttgcggtc gtcgcccacg ccatcccctg tttggcgcct tgcggcgtgt acgctcccga
      361 aaccggcgtt tcggccagcg ccatcgcctt ggcgatcgta gccggcgttc cggcccgctg
      421 catcgcggcg gagagatagc gcgtcatatc gggcagcgtc gagcgcagcg tgccggccgc
      481 cggccacgca tcgatcggcc agtgttgcac cgcgagcgtg ccggtacagt cgtatccttg
      541 cgcgtagttc ttagccggca cgtcgtacac gtcgtacgtt cgccgcatgt ttagcggcag
      601 cagcagcgac tgcgtcactt gctcgaacca tagtttctta ttgatgcctt gcagcacgta
      661 gccgagcgtg ccgaatccga tgttcgaata gagccatggg tgaggcggcg tgggagcggg
      721 gagcggactc ggataggttt cgacaaaatt gatgagttgg ttcggtgaat agcagggccg
      781 ctccgacaac gcaccttctt tcggcggcac gtcgggcagt ccggacgtat gggtggcgag
      841 catcgtcagc gtcatcgttg ccatcacgcc gtacgcgctc ggaggaaccg gcgtcggagc
      901 cggcggcggc gagcatccgg gcccgacgtc gttaccgtgt ccgagcgtct tgatgaaacg
      961 cgcggggtta tcgccgccct tcaggctgcc gttcgcgtag gcgagcgcga gcagtgcggc
     1021 ggtaaaagtt ttcgtaagcg atcctagctc gacgatcgag ccggcggtca tgcatcgccc
     1081 ggtccgcacg tcggcatagc cgctatagta ttgatagacg gcattcttgt agacgagcga
     1141 tatcgcgagc cctgggacgc cctgcacctt cgaaatgcct tcggcgaagg ccgaggcgac
     1201 gggcgagggc agcggcgacg gcaccgtggt cagacaggcc gcgccgccga tcgggttggg
     1261 cgtgggcggc tgacttaccg ttcccgacga gctgcagccg gctgcgacgg tgcacgacgc
     1321 gacgaaggcg agggcgacgt acggccgcca cgcgtgggaa gcgcgcatct ctcgcccatt
     1381 cgggcggacg ccgcgggcct ccgcccggcg ccacgttgaa gtatcgcgac cgtagaagcg
     1441 gtcggaaagg atgctggtca tcggggccgt accggctgtt tgcgaatccc gatttatttc
     1501 gcggacaatt tcctatttcg aagcctgcat cgtctcgagc tggaccttaa gcgggctgag
     1561 attgacggta acgaatccgg tctgcggccg gtcgtagtcg atcatcatcg tgacgacgat
     1621 gacgcttgcg accacgtaaa cggcgctgag cgccgcggga cgtactccgt ccgccgcaaa
     1681 gcgcaggccg agcgacagcg ctcccaacgt gtagagcgcc aacgacagct cgatcaccgc
     1741 ggttgggatg tggctcgtca gcgcctgaag ctgttcggcc gaaacgtcga tcgtgtcgtt
     1801 gagcgtttgc atcagcagtc cgagccccag gtcgcccgga tgggcgcgca aggcgggcga
     1861 gacgatcgcc cacatcgcat cttgatcgcg gttgctccgg tcgagatccg accggtacga
     1921 gactcccggc gaggctgcgg ggagttgata cgcttgcagc cgcgtcgagg tatagtcgac
     1981 caagatccgc cggaatcggc ggcgctggcc ttcgtcgagc tgatcggcgc gcagccaggt
     2041 ggtgccgatc gcgttggcct cccttattac cagcgcgcgc cgcgcgtcga agcgcccttc
     2101 cgcttgcgag aacgacaatc ccaacacgaa ggatgcgagc gtgagcaggc ccgcctgcac
     2161 cgccgtgagc gcgtccatct tgccggggat ccttaccttc agcagcagtc cggcccgaaa
     2221 gggaacctcg atcgcaaggc cgagcacgat cgtcaggacc acgaccagcc accacggatt
     2281 ggcaacgaaa atcaagtagt tcatgagacg ctttcgtagc tatgcgctcg cggaccgggc
     2341 gcgacctctc ccgcgacgcc ggccgcaaaa ctcgcgaacg gctaggtatt ctcgctttgt
     2401 gcgagatcga aggcttcccg taagaacggc aggatcgtcc gcaggtcgtc accggttcgc
     2461 acgagagcaa cgtgcgacac gcgcttcccg ctcgccccaa ctttgacgaa gcaaggcttt
     2521 ttgacgcggc gtgccaggtg aatcacgacg cgcagcgcat cccgcatcac ggtcagatcc
     2581 gcgaagatgc gggtcgttcg aaacaaggcg tagcggtcgc gagccacgat ttcgacgccg
     2641 ccgtaactgc gaaccgcggc ttcgagcttg cgatagagct cggccagctc gggcggacga
     2701 tttgccagca ggcttgcgtc atcgccggtt ccgcaggcgt gacgctgatt ggctcgacgg
     2761 aacgtccggc tgcagcgcgg gcatttccag gtcacccggt tgtcgtacac cgcaggcggc
     2821 gcaccgtcaa gagatgttcc gaaccgaaag ccggcgaagc ggtaagcgcg tgagcgccca
     2881 gctgttcgag gctttctatt cgttcgtgac gctgctcgcg atggtgaatc cgatcgaggc
     2941 cgcagcggcg tttacgacgc tcaccgcggg ccgttcgccg caggagcagg cgcgcatcgc
     3001 gctgcgggcg accgtcgtcg cggccgcgat tcttctcgga ttcagctatg ccggcgaggc
     3061 gctgctgaac gcgatgggta tctcgctggc ggctttcaag atcgcgggcg gactgctgct
     3121 gctgaaagtc ggcttcgaca tggtttttgc ggagaagagc gcgaccgggg gcgccggcgg
     3181 accggcaggg ccgccggcca gcgatccgtc ggtgtttccg ctcgccatcc cgctcatcag
     3241 cggccccggc gcgcttacgg ccggggtcac gctggtaagc cgggcgcacg aaaaccgcgt
     3301 gctggccgac ctcgccttcg tcgcgatcgc gctggtggtt ttcgcgatta cctacctggc
     3361 gatgcgcggc tccgaacgca tcacgaagct gctcggtccg atcggcgtcg acgcggcggg
     3421 acggctcgtc ggcatcatcg tgacggcgat cgcggtgcag ctggtcgtca acggcgccgg
     3481 cagtctcgtc cactacgtcg tgcgttagga cgcatcgaag acgacttggt cccattgcca
     3541 cagctgcagg aagaattcgc gcgcgaggtc gcggcttgcg agagggcggc aaatctctcc
     3601 gatcgtgcgc gcgccgtcga tgcctttcac gagctgctcg cgcagtttgt cgaccgggag
     3661 atagagatcg gtgtaggtgt ggttgcgatt gatgagcacc gcggtggacc cgggcggtag
     3721 ccggtcgcgc accgaaaccg tatccggcag ccggaccggg acgtaatcga gccaagcttc
     3781 gccctcgaag ttcggcgtat ccggcacgcc ggcgtcgtcg cgataggcga tgaagttgtg
     3841 gcgtaccatc gtcccgcgaa acaattccat ggccgcgaac cgttcgcgcg gcgacaggcg
     3901 gtcgaggagc gcgcggtgcg gagtcgccgc gacggcgccg cactcgggaa ggtacggcgc
     3961 ctggcgtatc cagcggacga agcgcaggcc ggcgcgctcg agaaacgcca tgacttgatc
     4021 gacgctgtac gcgcggtcgc gcggatgcag cagcgcgtcg gcgagcccgg ccgtgccggc
     4081 gaagtccggg gagttgcgca gcaaccgcac gatcg
//
LOCUS       DASUCJ010000622         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_21960, whole genome shotgun sequence.
ACCESSION   DASUCJ010000622 DASUCJ010000000
VERSION     DASUCJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21960"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="dusB"
                     /locus_tag="VFU90_07395"
     CDS             complement(<1..847)
                     /gene="dusB"
                     /locus_tag="VFU90_07395"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5479643.1"
                     /translation="MKIGPIAIWPPLVLAPMSGVTNRTMRALYKPFGLGLTVTEFVSA
                     NALQFGSRRTMEMIDQHGLEKPVSTQLWGDDPSTMAKAAKVVRECGADIVDINFGCPA
                     PKITKTNGGSACLRDPDRCEAIMRAVVAAVDCPVTMKMRLGWTENQLVYVEVARRAQA
                     AGVLAVTLHARTAKQFYKGDADWDHIARLKRSVDIPVIGNGDLDDAHVAMQRMRDSGV
                     DAIMLGRATLGNPWLISQIADLMEGRPAQPSPSPADRLRFSLVHYRTMVDELGEARAV
                     PQMRKH"
     gene            complement(886..1539)
                     /locus_tag="VFU90_07400"
     CDS             complement(886..1539)
                     /locus_tag="VFU90_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEU5479644.1"
                     /translation="MAGARTHVLLYTLTLVAGYGDAVAFFGLGVFTANMTGNTVLLGG
                     ALAGHLVRRLPGDIGIALPLLSLAGFVAGGSAAALFLRDERGRPPMRTAAVLLVVAIL
                     LGVTAGLQRWNGAGSVPAAVTVLSAVMGLQSIVAVRAGVEGVSTTFVTGTLVRSIMNL
                     LGTPTENALLQAEGRTNAWVWGCYLGGALVGAVALRTLGSDALWLPAVVVTLLLPVI"
     gene            complement(1546..2340)
                     /locus_tag="VFU90_07405"
     CDS             complement(1546..2340)
                     /locus_tag="VFU90_07405"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5479645.1"
                     /translation="MLLTIDVGNTETKLGCFSASGELAHTWRVTTDVRRTPDEYGAFF
                     MQLFASGGIDARAFDAVAIASVVPKLDPVLETVCARFFGAKPLFLKPQTQTLIPMGTA
                     YPEESGADLVAAAIGGRARFGAPLIVVSYGTATVFIAISGTGEYLGVAIAPGITISID
                     ALIGRTAKLPQIALEMPERAIGRTTNAALQAGIVYGFAGQTEAIVTRMRAELGEPAKV
                     VATGGLAELFAKHTPVVDAVDPHLSLAGLRLFYESVHGQGTSSEVP"
     gene            complement(2331..3518)
                     /gene="coaBC"
                     /locus_tag="VFU90_07410"
     CDS             complement(2331..3518)
                     /gene="coaBC"
                     /locus_tag="VFU90_07410"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5479646.1"
                     /translation="MKDARIFLGVAGGIAAYKAASLASNLVQRGALVDVVMTAEAERF
                     VAPLTFASLTARPVYTSLWDAPERIPHIRLIREAAVAIVAPATANLIAKLAAGLADDL
                     LAAALLAARIPVVLAPAMNDAMYAHPATRANLDTLRERGYEIVEPERGHLAEGESGIG
                     RLASEERLVEAVARALARRRSLEGKRVAITAGPTREAFDPVRFVSNASTGAMGIELAR
                     EAALRGAGVTLFLGPTLLEPPPGVTVVRFTTAGELYDAALAQAVGADLTLATAAVADW
                     RPERYSDSKVKKTDEDVTVRMVRNPDVLAALGERKRGTFLVGFAAETDDHERNAREKL
                     ARKDLDAIAVNDVRDERGFGTGENALVLLWGTDGRRDLGSAGKAALAARLLDAVEELM
                     ACS"
     gene            complement(3515..>4025)
                     /locus_tag="VFU90_07415"
     CDS             complement(3515..>4025)
                     /locus_tag="VFU90_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5479647.1"
                     /translation="TFEGVVRPTHFRGVATIVAKLLHVVRPDVLFLGQKDAQQTAVLR
                     KMVRDLAFGVTVEVVPTIRERDGLAMSSRNAYLTPEQRPQAASLHRALLAMRDVFERG
                     GSKGEAVTAARSALSPSATPDYFDVVDAESFEPVERPDGSAFIIGAARFGSIRLIDNL
                     HVTAGSDRP"
BASE COUNT          649 a         1429 c         1317 g          630 t
ORIGIN      
        1 cgtgtttgcg catttgcgga accgctcgcg cttcacccag ttcgtcgacc atcgttcgat
       61 agtgcacgag gctgaaccgc aagcgatcgg ccggcgacgg cgacggttgt gcgggccggc
      121 cttccatcag gtcggcaatc tgcgagatga gccacggatt tccgagcgtc gcgcggccga
      181 gcatgatcgc gtcgacgccg ctgtcgcgca tgcgttgcat cgcgacgtgc gcgtcgtcca
      241 ggtcgccgtt gccgatcacc gggatgtcga cgctgcgctt gagccgcgcg atgtgatccc
      301 aatcggcgtc gcccttgtag aactgtttgg cggtccgcgc gtgcagcgtt accgccaaca
      361 ccccggcggc ctgcgcgcgc cgcgcgactt ccacgtagac gagctggttt tcggtccagc
      421 cgagccgcat cttcatcgtc accggacagt cgaccgcggc caccaccgcg cgcatgatcg
      481 cttcgcagcg atcgggatcg cgcaagcacg ccgaaccgcc gttggtcttg gtgattttgg
      541 gcgccgggca gccgaagttg atgtcgacga tgtcggcgcc gcactcgcgc acgactttcg
      601 ccgccttcgc catcgtcgag ggatcgtcgc cccacagctg cgtcgaaacc ggcttttcga
      661 gaccgtgctg gtcgatcatc tccatcgtgc gccggctgcc gaactggagc gcgttggccg
      721 aaacgaactc ggtgacggtc agcccgaggc cgaacggctt gtagagcgcg cgcatcgtgc
      781 ggttggtcac gcccgacatc ggggcgagga ccagcggcgg ccaaatcgca atcgggccga
      841 ttttcaaggg ggcgggacgg tgagccgcag ccatggcgga acctactata tcacgggcag
      901 gagaagcgtc actaccaccg ccggcagcca cagcgcgtcg gaaccgagcg tgcgcagcgc
      961 gaccgcgccg accagcgctc cgcccaggta gcagccccac acccaggcgt tggtgcggcc
     1021 ttccgcttgc agcagcgcat tttcggtcgg ggtgccgagc aagttcatga tcgagcgcac
     1081 cagcgttccg gtgacgaacg ttgtcgagac gccttccacg ccggcccgca ccgccacgat
     1141 gctctgcagt cccatgaccg ccgaaagcac cgtcaccgcg gccggcaccg agcccgcgcc
     1201 gttccagcgc tgcagccccg cggtaacgcc gagcagaatc gcgaccacca gcagcaccgc
     1261 cgccgttcgc atcggcgggc gtccgcgttc gtcgcgcagg aagagtgcgg ccgcgcttcc
     1321 gccggccacg aaaccggcca acgacagcag cggtagcgcg atgccgatgt cgcccggcag
     1381 ccgccgcacc agatgaccgg cgagcgctcc gcccagcaag acggtgttgc cggtcatgtt
     1441 cgcggtgaac acgcccaaac cgaagaaggc caccgcgtcg ccgtagccgg cgaccagcgt
     1501 cagcgtgtaa agcagtacgt gagtgcgcgc gcccgccacc gccgtttaag gaacctccga
     1561 tgaggtcccc tgcccgtgga ccgattcgta gaacagccgc aagcccgcca agctcaagtg
     1621 cggatcgacg gcatcgacga ccggcgtgtg cttggcgaat agttcggcga gtccgccggt
     1681 cgcgaccacc ttcgccggtt cgcccaactc ggcgcgcatc cgcgttacga tcgcctcggt
     1741 ttgcccggca aatccgtaaa cgatccccgc ctgcagcgcc gcgttggtcg tccggccgat
     1801 cgcgcgttcc ggcatctcga gcgcgatctg cggcagtttg gcggtgcgcc cgatcagcgc
     1861 gtcgatcgaa atcgtgatcc cgggggcgat cgccactcct aaatattcac cggtgcccga
     1921 aatcgcaatg aacaccgtgg ccgtgccgta gctcaccacg atcaacggcg cgccgaagcg
     1981 cgcgcgcccg ccgatcgcgg cggccaccaa atcggcgccg gactcttccg gatatgccgt
     2041 ccccatcgga atcagcgtct gcgtctgcgg cttcaaaaac agcggcttcg caccgaaaaa
     2101 tcgcgcgcag acggtttcga gaaccggatc gagtttcggc acgacgctgg cgatcgctac
     2161 cgcgtcgaac gcgcgcgcgt cgatcccgcc cgacgcgaac agctgcatga agaacgcgcc
     2221 gtactcgtcg ggcgtccggc gcacgtcggt ggtcacgcgc caggtgtgcg cgagctcgcc
     2281 ggaagcgctg aagcagccga gtttggtttc ggtgttgcca acgtcgatcg ttaggagcat
     2341 gccatcagct cttcgacggc gtccagcagg cgcgccgcca gcgccgcctt gccggcgctc
     2401 ccgagatcgc gccgtccgtc ggtgccccac agcaggacga gcgcgttttc acccgtcccg
     2461 aagccgcgtt cgtcccgcac gtcgttgacg gcgatcgcat cgaggtcctt gcgcgcgagt
     2521 ttctcgcgcg cgttgcgttc gtgatcgtcg gtttccgcgg cgaagcccac caagaacgtg
     2581 ccgcgcttac gctcgcccag cgcggccagc acgtccggat tgcgcaccat tcggaccgtt
     2641 acgtcttcgt cggtcttctt caccttggag tcgctgtagc gttcggggcg ccagtccgcc
     2701 accgccgccg tcgcaagcgt gagatccgca ccgaccgcct gcgcgagcgc cgcatcgtag
     2761 agttcgccgg cggtggtgaa ccggacgacg gtcacgccgg gcggcggctc gagcagcgtc
     2821 gggccgagaa acagcgtcac gccggcgccg cgcagcgccg cttcgcgcgc cagctcgatg
     2881 cccatcgcgc cggtcgaagc gttgcttacg aaacgcaccg gatcgaatgc ctcgcgcgtc
     2941 ggtcccgcgg tgatcgccac ccgcttgcct tccagcgagc gccgccgcgc gagtgcgcgc
     3001 gcgaccgctt cgacgagccg ctcttccgaa gcgaggcggc cgatgccgct ctcgccttcg
     3061 gccagatggc cgcgctccgg ttcgacgatt tcgtagccgc gttcccgcag cgtatcgagg
     3121 ttcgcacgcg tcgccggatg cgcgtacatc gcatcgttca tcgccggcgc gagcacgacc
     3181 ggtatgcgcg ccgccagcag tgccgcggcg agcaggtcgt cggccaaacc ggccgcgagc
     3241 ttcgcgatca gattggccgt cgcgggcgcc acgatcgcaa ccgcggcctc gcgaatcagg
     3301 cgaatgtgcg ggatgcgttc gggcgcgtcc cacagcgacg tgtagacggg gcgcgcggtc
     3361 agcgacgcaa acgtcaacgg cgcgacgaac cgttcggctt cggccgtcat caccacgtcg
     3421 acgagcgctc cgcgctgcac caaattgctg gcaagcgatg cggctttgta ggcggcgatc
     3481 ccgcccgcga cgccgagaaa aatgcgtgcg tccttcacgg ccggtcgctg cccgcggtca
     3541 cgtgcaagtt gtcgatcagg cggatgctgc cgaaccgcgc cgcgccgata atgaaggccg
     3601 atccgtccgg ccgttcgacc ggctcgaacg attcggcgtc gacgacgtcg aagtaatccg
     3661 gcgtcgccga cgggctgagc gccgagcgcg ccgcggtgac cgcttcaccc ttcgaaccgc
     3721 cgcgctcgaa cacgtcgcgc attgccagca gcgcgcggtg cagcgacgcc gcctgcgggc
     3781 gctgttccgg cgtcagatac gcattgcggc tcgacatcgc gagcccgtcg cgttcgcgaa
     3841 tcgtcggcac gacctcgacg gtaacgccga aagcgaggtc gcggaccatc ttgcgcagca
     3901 cggcggtctg ctgcgcgtcc ttctgcccca agaacagcac gtcggggcga acgacgtgca
     3961 gcagtttggc gacgatcgtc gcgacgccgc ggaaatgcgt ggggcggacg acgccttcga
     4021 acgtc
//
LOCUS       DASUCJ010000623         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_709804, whole genome shotgun sequence.
ACCESSION   DASUCJ010000623 DASUCJ010000000
VERSION     DASUCJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709804"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFU90_07420"
     CDS             <1..220
                     /locus_tag="VFU90_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479648.1"
                     /translation="DMYFLRKRSIVNDHHWQNWVAALVPVARMPLAHKIFDNAVERNA
                     IDPEFAAFVRPLFEGGTLADPKGSAPGR"
     gene            263..>1792
                     /locus_tag="VFU90_07425"
     CDS             263..>1792
                     /locus_tag="VFU90_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479649.1"
                     /translation="MITVDEIRAVPIFSDLGEPELERLSKRSADIRLQAGEYAAHEGD
                     GRALFVVLEGEANVTRNVDGTERVVGKRVAGDVFGEVPIVLGTPFLASLRAATTVRLM
                     RIEPREFHAVCATEPELAAQIGALAKDRIEGLHDLAAEQPAPRATIVGDRYDSQCREL
                     RDFLDLNGVTFDWITPDDPELPARVPGFGGIKGRYPAVALDGGGLLIRPQQRELARRL
                     GLTTTPAFASYETVIIGGGPAGLAAAVYGASEGLRTLMIEREAPGGQAGTSSRIENYL
                     GFPNGVTGGELAGRALAQAKRFGAEILVTRSVIDIDHDTREIELDGGEVVNAGTIILA
                     TGVSWRRIPLEGFEELTGRGIYYGASQSEASTLAGLDVFLIGAGNSAGQAAMYFSSYA
                     RTVTLIVRGSSLEKSMSHYLVEQLGTKENVKIRLGAEVTAVHGSDHLETLDVTEKKTG
                     VTERVAAAALYVFIGADAETKWLPAAIDRDERGYILTGEEAGKSGNWRLQRDPYLLET
                     SV"
BASE COUNT          320 a          546 c          628 g          298 t
ORIGIN      
        1 agacatgtac ttcctgcgca agcggtcgat cgtcaacgac caccactggc aaaattgggt
       61 ggccgcgctg gtgccggttg cgcgcatgcc gctcgcgcac aaaatcttcg ataacgccgt
      121 cgagcgcaac gccatcgatc ccgagtttgc ggccttcgtg cggccgctgt tcgaaggcgg
      181 aacgcttgcg gatccgaagg gttcggcgcc gggccgttga gactcgaaag gccgttttgg
      241 tgcgggtaag aaccaccccg gcatgattac ggtcgacgaa atccgggcgg tgccgatttt
      301 ctcggatctc ggcgagccgg agctcgagcg gctctccaag cgatccgccg acattcgcct
      361 gcaggccggt gagtacgccg cgcacgaggg cgacggccgc gcgctgttcg tggtgctcga
      421 aggcgaagcc aacgtcacca gaaacgtcga cggcaccgag cgcgtcgtcg ggaagcgcgt
      481 cgccggcgac gttttcggtg aggttccgat cgtgctcggg acgccgtttc tggcgagcct
      541 tcgggccgcg acgacggtgc gtctgatgcg catcgaaccc cgcgaatttc acgccgtttg
      601 cgcgaccgag ccggagctgg cggcgcagat cggtgcgtta gcgaaagatc ggatcgaagg
      661 gctgcacgat cttgccgccg agcagccggc gccgcgggca acgatcgtcg gcgaccgtta
      721 cgattcgcag tgccgcgagc tgcgcgattt tctcgacctc aacggcgtca cgttcgactg
      781 gataacgccc gacgatcccg agcttccggc gcgcgtcccc ggcttcggag gcatcaaggg
      841 ccgatacccg gcggtcgcgc tcgacggcgg cggtcttctc atccggcccc agcagcgcga
      901 actcgcccgg cgtctcggct taacgacgac gccggcattt gcgagttacg agaccgttat
      961 catcggcggc gggccggccg gtctggccgc cgccgtttac ggcgcttcgg aagggctgcg
     1021 gacgctcatg atcgagcggg aagccccggg cggccaggcc ggtacgtcat cgcggatcga
     1081 aaattatctc ggctttccca acggggtgac cggcggcgaa ctggcgggcc gcgcgctcgc
     1141 gcaagccaag cgcttcggcg ccgaaattct ggtgacgcgc tcggtgatcg acatcgatca
     1201 cgacacgcgc gagatcgaac tcgacggcgg cgaggtcgtc aacgccggca cgattatttt
     1261 ggcgaccggc gtttcgtggc gccgcatccc gttggagggg ttcgaggagc tgacgggtcg
     1321 cggcatctat tacggcgcct cgcaaagtga agcgagcacg ctggcagggc tggacgtgtt
     1381 tctgatcggc gccggcaact cggcaggaca ggcggcgatg tatttttcga gctacgcgcg
     1441 cacggtaacg ctgatagtac ggggatcgtc gctggagaaa agcatgtcgc actatctcgt
     1501 cgagcagctc gggacgaaag aaaacgtaaa aatacggctg ggcgcggagg tcacggcggt
     1561 ccacggcagc gatcacttgg agacgctcga cgttaccgag aagaagacgg gcgtgacgga
     1621 acgggtcgcg gccgcggcgc tgtacgtgtt tatcggagcg gacgccgaga cgaagtggct
     1681 accggcggcg atcgatcgcg acgagcgcgg ttacatcctt acgggcgagg aggccgggaa
     1741 aagcggcaac tggcggctgc agcgagatcc gtatctgctc gagacgagcg ta
//
LOCUS       DASUCJ010000624         1316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_770934, whole genome shotgun sequence.
ACCESSION   DASUCJ010000624 DASUCJ010000000
VERSION     DASUCJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770934"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..521
                     /locus_tag="VFU90_07430"
     CDS             300..521
                     /locus_tag="VFU90_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479650.1"
                     /translation="MLKWGFVWFGLAVATGLVGATVHDARTFALAQILFFGCVVLFVV
                     FTLVGAVLPHPIDARGGEALPHHDEWFDR"
     gene            complement(666..935)
                     /locus_tag="VFU90_07435"
     CDS             complement(666..935)
                     /locus_tag="VFU90_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479651.1"
                     /translation="MMPIVLACATIALSACSSSSTTGAVAVSGKPGYPLPLSEPKITL
                     TAPAASHDYSPPPPNAGHVAPGTDATVALAADAKAAKAAAPAKSG"
     gene            1178..>1316
                     /gene="ssrA"
                     /locus_tag="VFU90_07440"
     tmRNA           1178..>1316
                     /gene="ssrA"
                     /locus_tag="VFU90_07440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          218 a          435 c          457 g          206 t
ORIGIN      
        1 cgagcgcgac cagcatgccg agaatgatcg gcaccaggac gcggaacggg agcagacgtt
       61 tcatgacagc agacgatacc tcccggcgca gggggaggac ggagacgcgc caatcgcaga
      121 gcgcgcggat cgaagtgaca cactatacgc ccgacgcgca ggcatgcgac ctcccgaggg
      181 gtaggtgttc gtccgacacg cggatgcgag accgcccgat accggcgccc cgcagcgcct
      241 cctagactcg ctccggtatg cgaacccagg gcacgaacga agcgacacct gcgacccgcg
      301 tgttgaagtg gggtttcgtg tggttcggac tcgccgttgc gaccggactg gtcggcgcaa
      361 cggtgcacga tgcgcggacc ttcgcactcg cgcaaattct gttcttcggc tgcgtcgtgc
      421 tgttcgtcgt cttcacgctc gtcggcgcag tgctgccgca cccgatcgat gcgcgcggcg
      481 gcgaggcgct gccgcaccac gacgaatggt tcgatcgctg acgatgccgg acgctgcggc
      541 ggcccgcgcg tcctgacggc tccctccccc tcgcgcccaa gcgaggggac ctcgacgccg
      601 ccccagggcg gcgtcttttt tggcgtgctc aggtcacgtg ctcagcccga cctggcgtcc
      661 agtgctcagc ccgacttcgc cggtgctgcg gccttcgccg ccttcgcgtc ggcggcgagc
      721 gcgacagttg cgtcggtgcc gggcgcaacg tgtccggcgt tcggcggcgg cggactgtag
      781 tcgtgcgaag cggcaggcgc cgtcaacgtg atcttcggct ccgacagcgg tagcgggtat
      841 cccggcttac ccgagacggc aactgcgccg gtcgtcgacg acgagctgca ggcagagagc
      901 gcgatcgtcg cgcaggcgag gacgatgggc atcatagtga cgagggtcag cggcttcatg
      961 gttggctccg caatgcatgg cgatgcggtc caccgtagcg cgcggcggcg cgtacggcga
     1021 ccggcggcac cctcgacctc gcgtagggaa agtccgatat ccgacgcgcg aacgacgcgc
     1081 aaaggtcgtt ccggcgcaag cggcagcgcg acccgtgctg gtatgatcac gctctcagcg
     1141 cttgccggcg aaggccggcg gccccatatc ggataccggg ggtgtactgg tttcgacggg
     1201 ggtatcgaag cagcgcgggg catgccgagg cgcttgtcct cgtaaaactc gagcactcgc
     1261 aaacttaact gcgaacgacg aaagctacgc tctggcggct taatcccgcc ggacct
//
LOCUS       DASUCJ010000625         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_801740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000625 DASUCJ010000000
VERSION     DASUCJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1292
                     /gene="dnaN"
                     /locus_tag="VFU90_07445"
     CDS             177..1292
                     /gene="dnaN"
                     /locus_tag="VFU90_07445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5479652.1"
                     /translation="MKFTCNTKDIAAAVGAASKVVNAHTTVPILSNVLLQSEEGRIAV
                     RATDLELTLEHAFAAEVGEDGSVTVPAKLFSSYLGNLPAGMLELTGTPTRASVKCERS
                     NYDFHALPADEYPPLPAATRGSHFTIGGKKFRDGIDATIFAASGEEARGAVLMGTLLE
                     VEGNALTMVATDGYRLAKFATTLDDGIAGSEKYIVPSRALAEVARNLGGSEPIAVSAL
                     GAQSNQLQMTAGSVSITVRLVDGQYPNYQQVIPAKFDRTMTVNTSALIGSLRRAELVA
                     GDRASMVKLAMGNQTLVVTANSDISGTAYEELEVEQTGEDLTIAFNARYLVEILNHIK
                     SERTVVEFLGPLSPAAIRPLEPLDSGQQLYVLMPLRQ"
     gene            1403..>2006
                     /locus_tag="VFU90_07450"
     CDS             1403..>2006
                     /locus_tag="VFU90_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479653.1"
                     /translation="MRITLADFRNYATLEHEPAAGLNVYVGANAQGKSNLLEAVAMLG
                     TGKSFRTARDGDAVRYGAESATVRGEALVRAGAVDLACAIAKSPVGTRKRYTLNGRDV
                     RYGSFLGKLRVVTFVPADLLLAGGAPGLRRAFLNAALSQEDPRYYRELARYRKAVSQK
                     NALLRGAIEPDSQLLRVYDTALLESGTQIVLARERFVRALS"
BASE COUNT          372 a          633 c          677 g          324 t
ORIGIN      
        1 cagccgtgga aatgtgcatt cgcggggcgg tcccgtccac aggttattct cggcggaaag
       61 ccgcacggtg cggggcgttt gggcactttt ccgcagaatg caccgcccta ctgctgttac
      121 gtctcaatac ataattcttt acccggaaaa cgcgcccggc tccagaacgg tggaagatga
      181 agttcacctg taacaccaag gacatcgcag cggccgtcgg agccgcgagc aaggtggtca
      241 acgcacacac gaccgtcccg atcctctcca acgtgctgct gcagtcggag gaaggccgga
      301 tcgcggtacg ggcgacggac ctcgagctca cgctcgagca cgcgttcgcg gccgaagtcg
      361 gcgaggacgg ctcggtgacc gtgccggcca agctgttttc gagttatctc gggaacctgc
      421 cggcggggat gctcgagctg accggaacgc cgacgcgcgc cagcgttaag tgcgagcgca
      481 gcaactacga cttccacgcg ctcccggccg acgagtatcc gccgctgccg gccgcgaccc
      541 gcggatcgca cttcacgatc ggcgggaaaa agtttcgcga cggcatcgac gccacgatct
      601 tcgccgcgtc cggcgaggag gcgcgcggcg ccgtgctgat gggcacgctg ctcgaggtcg
      661 aaggcaacgc cctaacgatg gtcgcgaccg acggataccg gctggccaag tttgcgacca
      721 cgctcgacga cgggattgcc ggaagcgaga agtacatcgt gccgtcgcgc gcactcgccg
      781 aggtcgcgcg aaacttgggc ggaagcgagc cgattgcggt gagcgcgctg ggcgcgcaga
      841 gcaatcagct gcagatgaca gccggaagcg tttcgattac ggtgcggctg gtcgacgggc
      901 agtatcccaa ctaccagcaa gtcataccgg cgaagttcga ccgcacgatg accgtcaaca
      961 cgagcgcgct gatcggcagc ctgcggcggg cggagctcgt cgccggcgac cgcgccagca
     1021 tggtgaagct cgcgatgggg aatcagacgc tcgtcgtcac cgcaaattcc gacatttcgg
     1081 ggactgcgta cgaagaactc gaggtggaac aaaccggcga ggatctcacg atcgccttta
     1141 acgcccggta tctggtcgag attctcaacc acatcaaaag cgaacgaacc gtcgtcgagt
     1201 tcttggggcc gctctcaccg gcggcgattc gaccgctgga gccgctcgat tccgggcagc
     1261 agctctacgt gctgatgccg ttgcggcaat gagccgcacg gggcgccggt atgagcagct
     1321 tcgaatcggc ggcgcgggcc gccgtttcac ttttcgcgcc tccgccggcg gtgcggacgc
     1381 acgctgagcg aggatgcgcc tcatgcgaat aacgctcgcc gatttccgga actacgcgac
     1441 gctggagcac gaaccggcgg cggggctgaa cgtctacgtc ggcgcgaacg cgcagggaaa
     1501 gagcaatttg ctcgaggccg tcgcgatgct cggcaccgga aaatcgtttc gtaccgctcg
     1561 cgacggcgac gcggtgcgct acggcgcgga atccgcgacc gtccgcggcg aggcgctcgt
     1621 gcgcgccggg gcggtcgatc tggcgtgtgc gatcgcgaag agccccgtcg ggacgcgcaa
     1681 acgctacacc ttgaacggtc gcgacgtgcg ctacggaagc ttcttaggca agctgcgcgt
     1741 cgtgacgttc gtaccggcgg atttgctttt ggcgggcggc gcgccgggtc tgcgccgggc
     1801 ctttctcaac gcggcgctct cgcaagaaga tccgcgctac taccgggagc tggcgcgtta
     1861 ccggaaagca gtttcgcaga agaacgcgct gctgcgcggc gcgatcgagc cggattcgca
     1921 gctcttgcgc gtatacgata cggcgctgct cgaaagcgga acgcaaatcg tactcgcccg
     1981 ggaacgtttc gtccgggcct tgagcg
//
LOCUS       DASUCJ010000626         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1290448, whole genome shotgun sequence.
ACCESSION   DASUCJ010000626 DASUCJ010000000
VERSION     DASUCJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290448"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFU90_07455"
     CDS             complement(<1..960)
                     /locus_tag="VFU90_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5479654.1"
                     /translation="MADSFHPLVSDWFAERFGQPTEPQVRGWPSIAAGGDTLISAPTG
                     SGKTLAAFLMCLDDLVRRSAAGNLPDETLVVYVSPLKALTNDVRKNLELPLAELVARA
                     AAANVPLMPIRTAVRTGDTTAAERQRMLRTPPHVLVTTPESLYILLTAEKPRALFANV
                     STVIVDEIHAVANSKRGSHLMLTLARLDDLVTAGGRSKPQRVGLSATIRPLERIANFL
                     SASPAIVDVGSRRAMGLSVEVPDEELGAVASGEMWAQIYDDVAEQILANRTTLVFVPT
                     RRMSERVAYALEQRLGEGVVMPHHGSLARDVRFDAENRLKNGEL"
     gene            1144..>1549
                     /locus_tag="VFU90_07460"
     CDS             1144..>1549
                     /locus_tag="VFU90_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479655.1"
                     /translation="MRIAQTPAASGNLLERLLAITHDILQARDIVPSLESIAESVSLL
                     FGFRYVTIVAADEPGGDLYRRVLLGARPGANNDRLGERISRDDIKTLLLPEFEVLPHC
                     FYNPVERDTSQSPRQAPDEWHERDSLTLVLPDR"
BASE COUNT          249 a          530 c          523 g          247 t
ORIGIN      
        1 cagctcgccg ttcttgagac gattttcagc gtcgaagcgc acgtcgcgcg ccaggcttcc
       61 gtggtgcggc atgacgacgc cttcacccag ccgctgctcg agtgcgtaag cgacgcgttc
      121 gctcatgcgc cgggtcggca cgaacacgag cgtcgtccgg ttggcgagaa tttgctcggc
      181 gacgtcgtcg tagatttgcg cccacatctc gccgctcgca accgcgccca gttcttcgtc
      241 gggcacctcg accgagaggc ccatcgcacg ccggctgccg acgtcgacga tcgccggcga
      301 cgcgctcaag aagttcgcga tgcgctccag cggccgaatg gtcgccgaaa ggccgacccg
      361 ctgcggtttg ctccggccgc cggcggtcac caaatcgtcc agccgcgcga gcgtcaacat
      421 gagatgcgag ccgcgcttgc tgttggcgac ggcgtgaatt tcgtcaacga tgacggtcga
      481 aacgttggcg aacagcgcgc gcggcttttc ggcggtgagc agaatataca gcgattccgg
      541 cgtggtgacg agcacgtgcg gcggggtccg cagcatgcgc tgacgctcgg cggcggtggt
      601 gtcgccggtg cgcaccgcgg tgcgaatcgg catcagcggc acgtttgcgg cagcggcgcg
      661 cgcgaccagc tcggccagcg gcagttccaa gttcttgcgg acgtcgttgg tcagcgcttt
      721 gagcggcgat acgtagacga ccagcgtttc gtcgggcaag ttgcccgcgg ccgaccgtcg
      781 cacgaggtcg tccaagcaca tcaggaacgc ggccagcgtc ttgcccgagc cggtcggggc
      841 ggagatcagc gtatcgccgc cggccgcgat cgacggccag ccccggactt gcggttcggt
      901 cggctgaccg aagcgctcgg cgaaccaatc gctgacgagc ggatggaaag agtcggccat
      961 gttcaccctt caaccccgcc gcgcccggcg gcgtttcggt cgccgccgcg cccgggcagc
     1021 cgttgccgga ggcgtgcgat taccggcgcc cgaatggctt acccgggcac gggtcgcccg
     1081 gagcacgcga gctcgggacg gcgtggcgag ccgcgggcaa aaactcggtc accgacgagg
     1141 aagatgcgga tcgcgcagac ccccgccgcg agcggcaatc tcttagaacg gctgctcgcg
     1201 atcacccacg acatcctgca ggcgcgggac atcgttccgt cgctcgaatc gatcgccgag
     1261 agcgtttcgc tgctgttcgg attccgctac gtcacgatcg tcgccgccga cgaaccgggc
     1321 ggcgatctct atcgccgcgt gctgctcggg gcacggccgg gcgccaacaa cgaccgtttg
     1381 ggcgagcgca tctcgcgcga cgacatcaag acgcttctgc tgcccgagtt cgaagtgctc
     1441 ccgcactgct tttacaatcc ggtcgaacgc gacacgtcgc agtcgccgcg ccaagcgccc
     1501 gacgagtggc acgaacgcga ctcgctcacg ctcgtgctgc ccgatcgcg
//
LOCUS       DASUCJ010000627         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_22034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000627 DASUCJ010000000
VERSION     DASUCJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="rplB"
                     /locus_tag="VFU90_07465"
     CDS             complement(<1..561)
                     /gene="rplB"
                     /locus_tag="VFU90_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5479656.1"
                     /translation="MPVKKYRPTSPGRRFITTMDFSELSAAGPERSLVEVKKKHSGRN
                     NNGHITVRRKGGGTRTQYRIIDFKRSKDGIPAKIATIEYDPNRSCRIALVHYRDGEKR
                     YILAPLGLKVGDLVESGPSADIKIGNALPIRNIPVGTVIHNVELRPGQGGKLVRSAGV
                     SAQLMAKEDDYSQIRMPSGEVRKVHVE"
     gene            complement(590..898)
                     /gene="rplW"
                     /locus_tag="VFU90_07470"
     CDS             complement(590..898)
                     /gene="rplW"
                     /locus_tag="VFU90_07470"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5479657.1"
                     /translation="MDARDVIIAPRITEKSMAEALANQYTFSVNPHATKTQIRHAIEE
                     IFKVNVTKVNTVNVRGKSRTFARRGRRTTGKQSDTKKAVVTLKPGQKIELGGVNYFEQ
                     "
     gene            complement(898..1557)
                     /gene="rplD"
                     /locus_tag="VFU90_07475"
     CDS             complement(898..1557)
                     /gene="rplD"
                     /locus_tag="VFU90_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5479658.1"
                     /translation="MPNVIDARGNVVREVATPAVFEPDYSDRVHVMYRAVHREFAGAR
                     AGTASTKKRDEVSGGGRKPWKQKGTGRARQGSIRSPQWRHGGVVFGPQPRSYVESLNK
                     KERRLAFVAALSERFKSGAVTLLDAGEFPIAKTSELATLLFGNPKNAKSGPTTLIVCA
                     RGETHGDAIVRVGRNLRRVGVTHDGALDVKDVLRYERIVFTTGGYDAVVGKLDERKES
                     E"
     gene            complement(1562..2200)
                     /gene="rplC"
                     /locus_tag="VFU90_07480"
     CDS             complement(1562..2200)
                     /gene="rplC"
                     /locus_tag="VFU90_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5479659.1"
                     /translation="MKNILGRKVGMTSVFTEDGRYVPVTVIEAGPCTVVERRTKEKHG
                     YEAIALGFGTVKKRRLTRALAGHYKKQGVDPVRYVREFRDDIEGVEAGATVTVAEFEA
                     GDRVDVVGVSKGHGFSGGIKRHNFSGGGASHGSMIHRQPASNGDTNAGRTVKGSRRPG
                     HYGVDRTTLQNLEVVRADAERNLLLIRGPVPGAKNALVIVRKSVKSAQAAGA"
     gene            complement(2369..3559)
                     /locus_tag="VFU90_07485"
     CDS             complement(2369..3559)
                     /locus_tag="VFU90_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5479660.1"
                     /translation="MTAAAGRRAASPDAIDFIDVASQLSDEERLIRDTTRAFVRANVL
                     PNVAEWFEEGVLPADLGRELGKLGLLGMHLHGFGCAGASAVAYGLACMELEAGDSGVR
                     SFASVQGSLAMYALYRWGDDAQKAAWLPHMASGETIGCFGLTEPDFGSNPAGMRTTAR
                     RDGGDWVLNGTKMWITNGTIAQVAIVWAQTGDGIRGFIVPAGTKGFTATNIHRKLSLR
                     ASVTSELVFQDCRLPAESMLAEARGLSGPLACLNEARYGIVWGAMGAARTCYETALDY
                     AKTRRQFDRPIGGFQLTQAKLANMLLELNKGTLLALHLGRMKDEGRLHPAQVSFGKLN
                     NVREALAIAREARTVLGANGVTLEYPVIRHMNNLESVLTYEGTSEMHALIVGKEITGL
                     DGFT"
     gene            complement(3556..4542)
                     /locus_tag="VFU90_07490"
     CDS             complement(3556..4542)
                     /locus_tag="VFU90_07490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5479661.1"
                     /translation="MIQRRLGKNGPFVSALGLGCMGMSDFYGPRDEAESLRTIRRALD
                     LGVTMLDTADMYGHGENERLVGKAIAKRRDGVFLATKFGIVRDPEEPSKRSFNGRPDY
                     VRSACEASLRRLGVDCIDLYYQHRVDLDVPIEETVGAMSELVKAGKVRYIGLSEASAP
                     TLRRAAAIAPIAAVQNEWSLWSRDLEDNGQLAAARECGASVVAYSPLGRGFLTGGIDS
                     PETLAPDDFRRSAPRFVGENFEKNRELVRQVAALAERKGCSASQIALAWVLARGDDVL
                     AIPGTKRVRYLEENLAALDVDLSAGDLESLHAIFPPGAASGARYPDMSFVNR"
     gene            complement(4560..4985)
                     /locus_tag="VFU90_07495"
     CDS             complement(4560..4985)
                     /locus_tag="VFU90_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479662.1"
                     /translation="MPIELHETRSRACHGTKRRIYHLLEEIGTSGDQIWPFASQPFMR
                     SPGPLIPGKTEEWHLGMHGILEEAAPEERIVWRIQNEGIDGTHGFHLATEGKETVLLY
                     RIDAVLSDTDGRLLWRRFEDQFERSTEALFDKLARVLKR"
     gene            complement(5030..>5228)
                     /locus_tag="VFU90_07500"
     CDS             complement(5030..>5228)
                     /locus_tag="VFU90_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479663.1"
                     /translation="IVGLVWSSVWTIGRGTTVVTLPPGSLGMVVSSAIVFAVTLLMLR
                     SKLGAPLLILLSGVVGIFALR"
BASE COUNT          850 a         1763 c         1586 g         1029 t
ORIGIN      
        1 ctcgacgtga accttgcgca cctctcccga cggcatgcgg atttgggagt agtcgtcttc
       61 tttggccatc agctgcgccg aaacgcccgc ggagcgtacg agctttccgc cctggccggg
      121 ccgcagctcg acgttgtgaa tgacggtgcc gaccgggatg ttccggatcg gcagcgcgtt
      181 gccgattttg atgtcggccg acggccccga ctcgacgaga tcgccgacct tcagtcccag
      241 gggtgccagg atgtagcgct tttctccgtc gcggtaatga acgagcgcga ttctgcacga
      301 ccggttcgga tcgtattcga tcgttgcgat cttcgccgga ataccgtctt tcgaacgttt
      361 gaagtcgatg atccggtact gcgtgcgggt cccgccgcct ttacggcgca ccgtaatatg
      421 gccgttgttg ttccgtcccg aatgcttctt cttcacctcg acgagcgagc gctccggccc
      481 cgccgccgac agctccgaga agtccatcgt cgtgatgaag cgccgccccg gcgacgtcgg
      541 tcgatatttc ttgactggca ctaccttgcg cccccaattc gcctgctcgt cattgctcga
      601 agtaattcac tccgccgagt tcgatctttt gcccgggctt cagcgtgacc acggccttct
      661 tcgtatcgct ctgcttgccg gtcgtccggc gcccccggcg cgcgaacgtt ctggacttac
      721 cgcgcacgtt cacggtgttg accttggtga cgtttacctt gaatatctct tcgatcgcat
      781 gccggatttg ggttttcgtc gcatgcggat taacggaaaa cgtgtactga ttggccagcg
      841 cttcggccat cgatttctcg gtgatgcgcg gagcgataat cacgtcgcgg gcgtccatta
      901 ttcgctctcc ttccgctcgt caagctttcc gacgacggcg tcgtacccgc cggtggtaaa
      961 gacgatccgc tcgtagcgca ggacgtcttt gacgtccagc gcgccgtcgt gcgtaacgcc
     1021 gacgcggcgg aggttccggc ccacccggac gatcgcgtcg ccgtgcgttt cgccgcgagc
     1081 gcacacgata agcgtcgtgg gaccgctctt ggcgtttttc ggatttccga agagcagcgt
     1141 cgccagttcc gaggttttcg cgatcggaaa ctcgccggcg tcgagcagcg tcaccgcgcc
     1201 gctcttgaat cgttcggaga gtgcggctac gaacgcgagc cgccgttcct ttttattgag
     1261 cgattccacg tagctgcgcg gctggggtcc gaagaccacg ccgccgtggc gccactgcgg
     1321 cgaccggatg gacccctggc gtgcccgccc ggtccccttt tgtttccacg gcttgcggcc
     1381 gccgcccgaa acttcgtcgc gtttcttggt cgatgccgtg ccggcgcgcg cgccggcaaa
     1441 ctcgcggtgt acggcccggt acatcacgtg gacgcggtcg ctatagtccg gctcgaaaac
     1501 cgccggggtg gctacttcgc ggacgacgtt tccgcgggcg tcgataacgt tcggcatcgc
     1561 gttaggctcc cgccgcctgg gccgacttca cactcttgcg gacgatcacc agcgcgtttt
     1621 tcgccccggg aacggggccg cggatcagga gtaaatttct ctcggcatcg gcgcgaacga
     1681 cctcgaggtt ctgcaaggtc gtccggtcca cgccgtaatg ccccggccgg cggctacctt
     1741 tcacggttcg cccggcgttg gtatcgccgt tcgacgccgg ttgccggtgg atcatcgatc
     1801 cgtggctcgc gccgccgccg ctaaagttat ggcgcttgat gccgccggag aaaccgtggc
     1861 ccttcgacac gcctacgacg tcgacgcgat cgcccgcttc gaactccgcg acggtgaccg
     1921 ttgcgccggc ttcgacgccc tcgatatcgt cccgaaactc gcgcacgtac cgcaccggat
     1981 cgactccttg cttcttataa tgtccggcaa gggcgcgggt gagccggcgc tttttgaccg
     2041 tgccgaagcc caaagcgatg gcttcgtagc catgcttttc cttcgtgcgc cgctccacga
     2101 ccgtgcaggg accggcttcg atcaccgtga cggggacgta tcgcccgtcc tcggtgaaaa
     2161 cgctcgtcat cccaaccttg cggccaagga tgttcttcat gacgtcctcg tcctacggaa
     2221 aatgtgactt taatgacacg ggcccaggcg tactggcctt agctttttcc ggcgcgcaaa
     2281 gcaccgccgc ggccggcctg gaccacaacc tgggcagtgt atcaggcgcc gctttccccg
     2341 tcaaggctca ccggcgtttg ggccggagct aggtgaagcc gtcgaggccg gtgatctcct
     2401 tcccgacgat cagcgcgtgc atctcgctgg tgccctcgta ggtcagcacc gattcgaggt
     2461 tgttcatgtg gcggatcacg gggtactcga gggtgacgcc gttggcgccc aggacggtcc
     2521 gagcctcgcg ggcgattgcc agcgcctcgc gcacgttgtt cagcttcccg aagctcacct
     2581 gggccgggtg cagccgcccc tcgtccttca ttctaccgag gtgcagcgcc agcagcgttc
     2641 ccttgttcag ctcgagcagc atgttagcga gcttggcctg cgtcagctgg aacccgccga
     2701 tcgggcggtc gaactgccgg cgcgtcttcg cgtaatccag cgccgtttcg taacacgtcc
     2761 gcgctgcccc catggcgccc caaacgatgc cgtagcgcgc ttcgttcaga caggcgagcg
     2821 gtccgctcaa gccgcgcgct tccgccagca tcgactcggc ggggagacgg caatcttgaa
     2881 aaaccagctc gctcgtcaca gacgcgcgca gcgataattt tcgatggatg ttcgtcgcgg
     2941 tgaagccctt cgttccggcg ggcacgatga atccgcggat gccgtccccg gtctgcgccc
     3001 agacgattgc cacctgcgcg atcgtgccgt tggtgatcca catcttcgtc ccgttgagca
     3061 cccaatcgcc gccgtcgcgg cgcgcggtgg tgcgcatccc cgcggggttg cttccgaaat
     3121 ccggctccgt cagcccgaag catccaatcg tttcgccgga tgccatgtgc ggcagccagg
     3181 ccgctttttg cgcgtcgtcg ccccagcggt agagcgcata catcgcgagc gagccctgca
     3241 ccgaggcaaa actgcgaacg ccgctgtcgc ccgcttccag ctccatgcac gcaagcccgt
     3301 aggcgaccgc actggcgccc gcgcagccga aaccgtgcag gtgcatcccc aacaagccga
     3361 gctttccgag ttcgcgcccg agatccgcgg gcagcacgcc ttcctcaaac cattcggcga
     3421 cgttgggcaa gacgttcgct cgtacgaacg cgcgcgtcgt gtcgcgaatc aggcgttcct
     3481 cgtccgaaag ctggctcgcg acgtcgatga agtcgatcgc gtcgggcgag gccgcgcgcc
     3541 gaccagccgc tgccgtcacc ggttgacgaa actcatatcg ggataacgcg ctccgctggc
     3601 ggcccccggc gggaagatcg cgtgcagcga ttcgaggtcg ccggcggaaa ggtcgacgtc
     3661 gagcgccgcg aggttttcct ctagataacg cacgcgtttg gttccgggaa tcgcaaggac
     3721 gtcgtcgccg cgtgccaaca cccatgcgag tgctatttgc gatgcggaac agcccttgcg
     3781 ctcggcgagc gccgcgacct gtcgcaccag ctcccgattc ttttcgaaat tctcacccac
     3841 gaatcgcggc gcgctgcgcc ggaaatcgtc gggcgccagc gtctccgggc tatcgatccc
     3901 accggtcaag aatccccgtc cgagcgggct atacgcgacc acgctcgcgc cgcactcccg
     3961 tgccgccgcc agctgtccgt tatcctcgag atcgcgcgac cagagcgacc attcgttttg
     4021 caccgccgcg atcggcgcga tcgcggccgc ccggcgcagc gtcggcgcgc tcgcttcgga
     4081 taggccaatg tagcgaacct tcccggcctt gaccagctcg ctcatcgcac cgaccgtttc
     4141 ctcgatcggc acgtcgagat cgacgcgatg ctggtagtac aagtcgatgc aatcgacgcc
     4201 tagacggcgc agcgaagctt cgcacgccga gcgcacgtag tcgggccggc cgttgaagct
     4261 gcgcttcgag ggttcctcgg gatcgcggac gatgccgaac ttcgtcgcca aaaagacccc
     4321 gtcgcgacgc ttggcaatcg cttttccgac gagacgctcg ttttcgccgt gtccgtacat
     4381 atccgccgtg tcgagcatcg tgacgccgag atccaacgct cgccggatcg tacgcaacga
     4441 ctcggcttcg tcgcggggac cgtagaaatc cgacatcccc atgcagccca aacccagcgc
     4501 cgaaacgaac gggccgtttt ttcctaaccg tcgttgaatc atgtcctgta ggaaaccggt
     4561 cagcgtttca agacgcgtgc gagtttgtcg aacagcgcct cggtggagcg ttcgaactga
     4621 tcctcgaagc gtcgccacag cagccggccg tcggtgtccg aaagcaccgc gtcgatccga
     4681 tacaacagta cggtttcttt gccttcggtc gcgagatgga atccgtgcgt accgtcgatg
     4741 ccttcgtttt gaatgcgcca gacgattctt tcctccggcg ccgcttcttc gaggattccg
     4801 tgcatgccga ggtgccactc ctcggttttc ccgggtatca gcgggccggg ggaacgcatg
     4861 aagggctgcg aggcgaacgg ccagatctga tcgcccgacg ttccaatctc ctcgaggaga
     4921 tggtaaatcc ggcgcttcgt cccatgacac gcacgcgacc gggtttcgtg gagttcgatc
     4981 ggcatggtcc gtcgttcgtt aggggccagc caagactcct agcagaccgc tagcgcaagg
     5041 cgaagatccc cacgacgccg gagagcagga tcaacagcgg cgcgccgagc ttcgaacgca
     5101 gcatcagcag cgtcacggcg aagacgatcg cgctcgagac gaccatgccg agcgagcccg
     5161 gcggcagggt gacgaccgtg gttccgcgac cgatcgtcca tacgctcgac cacaccagtc
     5221 cgacgatc
//
LOCUS       DASUCJ010000628         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1061325, whole genome shotgun sequence.
ACCESSION   DASUCJ010000628 DASUCJ010000000
VERSION     DASUCJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061325"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="priA"
                     /locus_tag="VFU90_07505"
     CDS             <1..1094
                     /gene="priA"
                     /locus_tag="VFU90_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5479664.1"
                     /translation="ESYAAARAGKIGLIELRRRATGAPLPIVQIVDLAKEFEAGNRRM
                     FSTVLVQALAQRLKDGEKSVLFLNRRGSARFVLCRNCGLVPHCPRCSVSLAVHRSEGL
                     LRCHYCDYQSAISNACVSCGGTNVKEFGVGTEAVAEEVRRLFPQARVVRMDSDTTTRV
                     GDHARILTAFAGEGDVLVGTQMVAKGLDYPTVTLAAVVAADIGLHVPDFRAAERSFGL
                     IAQVCGRSGRTSAGEAIVQTYTPAHPAVAFGAEHDYDGFARRELEERKLLGFPPARRL
                     VYLGAIGRSRTEALTAARRYADALRELAGTEVLGPAPYPIARLNGEWRFRVAVKTARP
                     GALRAAIRAQVLPAARADRRTRLSINVDP"
BASE COUNT          213 a          401 c          459 g          195 t
ORIGIN      
        1 tggagagcta cgccgcagcg cgggccggaa agatcggcct gatcgagctg cggcggcgcg
       61 ctaccggcgc accgctgccg atcgtccaaa tcgtcgacct ggcgaaagag ttcgaggccg
      121 gaaaccggcg gatgttcagc accgttttag tacaagcgct cgcgcagcgg ctgaaagacg
      181 gcgagaagag cgtgctgttc ctgaaccggc gcggaagtgc gcgcttcgta ctgtgccgca
      241 actgcggact cgtgccgcac tgcccgcgct gcagcgtttc gctcgcggtc catcgcagcg
      301 aaggactgct gcgctgtcac tactgcgact atcagtccgc aatttcgaat gcgtgcgtct
      361 cgtgcggtgg aaccaacgtc aaggagttcg gagtcgggac cgaggccgtc gcggaagaag
      421 tgcggcggtt atttccgcaa gcccgggtcg tgcgcatgga ttccgacacg accacgcgcg
      481 tcggggatca cgcgcgcatc ctgacggcat ttgccggcga gggcgacgtc ctggtcggca
      541 cgcagatggt ggccaaaggg ctggactatc cgacggtcac gctggccgcc gtcgtagcgg
      601 cggacatcgg tctgcacgtt cccgattttc gcgccgccga gcgtagcttc ggactgatcg
      661 cgcaagtctg cggccgcagc ggacggacga gcgcgggcga ggcgatcgtc cagacgtaca
      721 cgccggcgca cccggccgtc gcgtttggag ccgagcacga ctacgacggc tttgcccggc
      781 gcgagctgga ggagcgcaag ctgctgggct ttccgcccgc gcggcggctc gtatacctcg
      841 gcgcgatcgg acgcagccgg accgaggcgc tcaccgcagc gcggcgttat gcggatgcac
      901 tgcgggaact ggcaggcacc gaggtgctag gcccggcgcc gtatccgatc gcgcggctca
      961 acggcgagtg gcgctttcgg gtggcggtaa aaacggcgcg accgggagcg ctccgggcgg
     1021 cgatccgggc gcaggttctc ccggcggcgc gtgcggaccg tcgcacgcgc ttatcgatca
     1081 acgtcgaccc gtagaggcta gcgcgaacgc ccggcgtgcg cgcggcggcg cagcgtcgaa
     1141 gccggaagga cgagctgcga cagcagcaag ccggcgacga tttgaatcgc cgcgacgatc
     1201 gtattgccgg ccaggatcaa cgcgctgtcg atgttgtgct ggaaggcgaa caggacgctt
     1261 tcgtagct
//
LOCUS       DASUCJ010000629         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1061352, whole genome shotgun sequence.
ACCESSION   DASUCJ010000629 DASUCJ010000000
VERSION     DASUCJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061352"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1809)
                     /locus_tag="VFU90_07510"
     CDS             complement(130..1809)
                     /locus_tag="VFU90_07510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5479665.1"
                     /translation="MIVPPPPAAPYVRVVPLGGCGEIGRNMTAIETNDDLVVVDCGLM
                     FPDDEMYGVDVVINDFTYVRERRAKLRALLVTHGHEDHIGGIPYFLRDFPDTPIVGTP
                     LSLALIKAKSRGQKTADAAFVEVRPGDRVTYGAIEAEFVHINHSVAGACALALRTPVG
                     TIFHTGDFKFDQTPIDGKPADFARIARIGDEGVLCMLSDSTNAERPGHTLSERIVGEA
                     FTTIFSRAKGRILIASFASNVPRIQQAVDHAVRFGRKVAFLGRSMQNVVHFSSELGHL
                     KIPPDTVIKIDEIDDHPPERLVVVTTGSQGEPMSGLARMSVRDHRKFRIVPGDTVVIS
                     ATPIPGNERSVYKTINNLAKLGATVIHGTDGRSHVSGHGSQEELLLMLNLIRPEFFVP
                     VHGEYRMLMQHGRLAVQTGVEPGNVYVVENGDVLEFTSEYADKIGKTYGGHVYVDGSG
                     VGDVGEAVLRDRKALSNDGIMVVVVAIDSEEARITAGPDLISKGVFYLAEAGGVLDEL
                     RAGLAASLAEISVEGMRDVDTVKEHIRSGLAQAIYARTKRRPVIVPVVMEV"
     gene            2234..>2431
                     /locus_tag="VFU90_07515"
     CDS             2234..>2431
                     /locus_tag="VFU90_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479666.1"
                     /translation="MGVDRAGAPKANGLKTALDTIISPKEAFEQLRVAPTWGWALLLT
                     IVLYAFASYLLTPAIVHGMQAD"
BASE COUNT          405 a          783 c          798 g          445 t
ORIGIN      
        1 agttcaccga cgccgtcatc gaaagcgttc gccgcaaact cgacgtttgg tcgacgttgt
       61 aagcgccggc tggtgtcagc gtccttcgac tgcggcgctg cgcgcctccg ctcaggatga
      121 cacccggggt taaacttcca tgactacggg gacgatgacg gggcggcgtt tggtgcgcgc
      181 gtagatcgcc tgcgccaggc cgctgcgaat gtgctctttg acggtgtcga cgtcgcgcat
      241 cccttccacc gatatttcgg cgagcgaggc ggccagcccg gcgcgcagct cgtcgagcac
      301 gccgccggct tccgctaagt agaagacgcc cttggaaatg agatcggggc cggcggtgat
      361 gcgcgcttcc tcgctgtcga tcgcgacgac caccaccatg atcccgtcgt ttgaaagcgc
      421 cttgcggtcg cgcagcacgg cttcgccgac gtccccgacg cccgaaccgt cgacgtagac
      481 gtggccgccg tacgtcttac cgatcttgtc ggcatactcg gaggtgaact cgagcacgtc
      541 gccgttctcg acgacgtaca cgtttccggg ctccacgccg gtctgcacgg cgagccggcc
      601 gtgctgcatc agcattcggt attcgccgtg aaccggaacg aagaactccg gacggatcaa
      661 gttgagcatc agcagcagct cttcttggga tccgtggccg gagacgtgcg agcgcccgtc
      721 ggttccgtgt atcacggtgg caccgagctt ggcgaggttg ttgatcgtct tgtacacgct
      781 gcgctcgttg ccgggaatcg gcgtggcgga gatcaccacc gtgtcgccgg gaacgatccg
      841 gaacttgcgg tggtcgcgta ccgacatccg ggcgagcccg ctcatcggct cgccttgcga
      901 accggtcgtc accacgacga gacgttcggg cggatggtcg tcgatctcgt cgattttgat
      961 gaccgtgtcc ggcggaatct tcagatgccc gagttcgctg gaaaagtgga cgacgttctg
     1021 catcgagcgc ccgaggaacg cgaccttgcg gccgaagcgg acggcgtggt cgacggcctg
     1081 ctggatgcgc ggcacgttcg aagcaaacga cgcgatcaga atccggcctt tcgcccgcga
     1141 gaagatcgtg gtaaaggctt cgccgacgat tcgctccgag agcgtgtgcc cgggccgctc
     1201 ggcgttggtg gagtcggaga gcatgcacag cacgccctcg tcgccgatcc gcgcgatccg
     1261 ggcgaaatcg gccggcttgc cgtcgatcgg cgtctggtcg aacttgaagt cgccggtgtg
     1321 aaagatcgtc ccgaccggcg ttcgcagcgc cagcgcgcag gcgcccgcta ccgaatggtt
     1381 gatgtgtacg aattccgcct cgatcgctcc gtacgtcacg cggtcgccgg gacgcacttc
     1441 gacgaacgcc gcgtcggcgg tcttctgccc ccgggatttg gctttgatca gcgccagcga
     1501 gagcggcgtt cccacgatcg gcgtgtcggg aaagtcgcgc agaaagtacg ggatgccgcc
     1561 gatgtgatct tcgtgcccgt gcgtgaccaa gagcgcgcgc agcttggcgc gccgctcgcg
     1621 gacgtaggtg aaatcgttga tgacgacgtc cacgccgtac atctcgtcgt cgggaaacat
     1681 caacccgcag tcgaccacga cgagatcgtc gttggtctcg atcgcggtca tgttgcggcc
     1741 gatttcgccg catccgccca gtggaacgac gcgcacgtac ggcgcggcgg gcggcggggg
     1801 aacgatcaac gtatccggat tcaattcatt cgggccttgc gctgcgcgag tccggcgtga
     1861 agtgagatcg aagccacctt tcgccggagg atcctatgcg agttctcgtt tcgatgctgc
     1921 tcgccgtctt caccgcggct gcgtcccggc cccttgggaa ggagacccct tcgccgaacc
     1981 ccgccgaaac gcacgctcct cgcgccgtcg aactgctctc gccgcgcgcg ctccggcgcg
     2041 cgcgtctgct gatacaactg cggatgctgg agctgcgcga cgaacggctc gaatgcgtgc
     2101 gcgcagcgat tctgcgccgg ctcccgcccg acttgcggcc gacaggcgag cctgcttcgg
     2161 ccgtgaaaca atccggtgca acgcacgcta gaaactctcg gggcgcgctt ccatttgaaa
     2221 gcgaggactg tggatgggtg tcgatagggc tggggcgccg aaagcaaacg gactaaaaac
     2281 ggcgctcgac acgatcatat cccccaagga agccttcgag cagctgcgcg tcgcgccgac
     2341 gtggggctgg gcgctgctgc tcacgatcgt gctctacgcc ttcgccagct atctgttgac
     2401 cccggcgatc gtgcacggca tgcaggccga c
//
LOCUS       DASUCJ010000630         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_939089, whole genome shotgun sequence.
ACCESSION   DASUCJ010000630 DASUCJ010000000
VERSION     DASUCJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939089"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFU90_07520"
     CDS             complement(<1..1007)
                     /locus_tag="VFU90_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5479667.1"
                     /translation="MPGMMDTVLNLGLNNASVQGLAKQTGNERFAWDAYRRFVQMFAT
                     VVLGMKREPFDEVVKRYIAKARIASESDLTALAFRNMTSEFKALVRHHTKKDFPEDVF
                     VQLRMAVEAVFNSWNSKRAIEYRKHERIPDWWGTAVNVVAMVFGNMGDDSGTGVAFTR
                     NPSTGDKKLYGEFLPNAQGEDVVSGSRTPFPIAELEKRNPKLYKQFADIAATLEKHYH
                     DMQDIEFTVERAKLYVLQCRSGKRSAAAAIQIAADMVKEHLIQREEALTRIDPASIQA
                     LLHAQIDDKAHATPLTKGLNAAPGAAAGIVVFDPDTAVEMRRENKKTILVRPMTTPDD
                     V"
BASE COUNT          155 a          319 c          349 g          215 t
ORIGIN      
        1 tggacgtcgt ccggcgtcgt catcggacgg acgaggatcg tcttcttgtt ctcgcgccgc
       61 atctcgacag cggtgtccgg gtcgaagacg acgatgccgg cggcagcccc gggcgcagca
      121 ttgaggccct tcgtgagcgg cgtggcgtgg gctttgtcgt cgatctgcgc gtggagcagc
      181 gcttggatcg acgccggatc gatgcgcgtg agcgcctctt cgcgctggat gagatgctct
      241 ttcaccatgt cggcggcgat ctggatggcc gccgcggcgc tccgcttgcc gctgcggcac
      301 tgcaggacgt agagcttggc gcgctcgacc gtgaactcga tgtcctgcat gtcgtggtag
      361 tgcttctcga gcgtcgcggc gatgtcggcg aactgtttgt agagcttcgg attgcgcttt
      421 tcgagctcgg cgatcgggaa cggcgtgcgg ctgccggaaa cgacgtcttc gccttgcgcg
      481 ttgggcaaga actcgccgta gagcttcttg tcgccggtcg acggattgcg cgtgaacgcg
      541 acgcccgtgc ccgagtcgtc gcccatgttg ccgaacacca tggccacgac gttcacggcg
      601 gtgccccacc agtcggggat gcgctcgtgc ttgcggtatt cgatcgcgcg cttgctgttc
      661 cacgagttga agaccgcttc gaccgccatg cgcagctgga cgaagacgtc ttccgggaag
      721 tccttcttcg tgtgatggcg gacgagcgct ttgaattcgc tcgtcatgtt gcggaacgcg
      781 agcgccgtga ggtccgactc cgacgcgatg cgcgccttgg cgatgtaacg cttgacgacc
      841 tcatcgaaag gctcgcgctt catgccgagc acgaccgtcg cgaacatctg gacgaaacgc
      901 cggtacgcat cccaggcgaa ccgttcgttg ccggtctgct tggcgagacc ctgaacggac
      961 gcgttgttca gtccgaggtt caggaccgtg tccatcatcc ccggcatgga gaatttcgcg
     1021 ccggagcgga cgctcacc
//
LOCUS       DASUCJ010000631         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_205682, whole genome shotgun sequence.
ACCESSION   DASUCJ010000631 DASUCJ010000000
VERSION     DASUCJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205682"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFU90_07525"
     CDS             <1..299
                     /locus_tag="VFU90_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5479668.1"
                     /translation="DGLTPVLVDDIISTGQTMIAAVEAVRQSLPASRPAVCIGVHALF
                     TRRVLDAMLAAGATRVVTTNTVPHISNGIDITGSVAAALRRQLAAPRTASIRAS"
     gene            349..477
                     /locus_tag="VFU90_07530"
     CDS             349..477
                     /locus_tag="VFU90_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479669.1"
                     /translation="MSEAIKQPASFQVPSSYPTTYSITHDAAQRVTRVTLDSLVAR"
     gene            complement(523..>1197)
                     /locus_tag="VFU90_07535"
     CDS             complement(523..>1197)
                     /locus_tag="VFU90_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479670.1"
                     /translation="AFEEDGRLVGFVFGLTGVEGGRLVHWSDMLAVREHLRDHGIGRR
                     LKEFQRDTVRKLGVSRIYWTFDPLVSRNAHFNLTRLGAHVVEYVPDMYGGETNSALHR
                     GVGTDRFIVAWDIDGAMHGVVGDDAANGRSPGADAPILNPIGDDGLPAVPNVAGHLPA
                     PMVRIAVPDEIERVQSQSVDNAARWRASTRRAFLWALEDGYRVSTFVRDDGAHYGFYV
                     CTRDHR"
BASE COUNT          198 a          423 c          391 g          185 t
ORIGIN      
        1 acgatggact cacaccggtc ctcgtggacg acatcatctc gactggtcaa acgatgatcg
       61 cggcggtgga agcggtgaga cagagccttc cggcgtcgcg gccggccgtc tgcatcggcg
      121 tccacgcgct gttcacgcgc cgcgtcctcg acgcgatgct cgccgcgggc gcgacacgcg
      181 tggtgacgac gaacaccgtt ccgcacatct cgaacggtat cgacatcacc ggctcggtcg
      241 cggcggcgct tcgccggcag ttggccgcgc cgcgcactgc ttcaatcagg gcgagttgac
      301 cttcatcgag ccgatggtgg cgacggcgta cttgcagcag caccggacgt gagcgaagcc
      361 atcaaacagc cggcatcctt tcaggtgccg agcagctacc cgacgaccta cagcatcacg
      421 cacgacgccg cgcagcgcgt gacgcgggtg acgttggact cgctcgtcgc acgatgagtc
      481 gtggggcgag ggaatcgcgc tccggcagga cggttccctc gcctaacgat gatctcgcgt
      541 gcacacgtag aacccgtagt gtgccccatc gtcccgaacg aaggtgctca cccgatagcc
      601 atcctcgagc gcccacagga acgcccgccg cgtgctcgcg cgccagcgcg ccgcgttgtc
      661 caccgactgc gactgcacgc gttcgatttc gtcgggtacc gcgattcgca ccatcggcgc
      721 cggtaggtgg ccggcgacat tcggaacggc tggcaacccg tcgtcgccga tcgggttgag
      781 aatcggcgca tcggcgccgg gcgacctgcc gttcgctgcg tcatcgccga cgacgccgtg
      841 catcgcgccg tcgatgtccc acgcgacgat gaaccggtcg gtgcccacgc cgcggtgcag
      901 cgcgctgttg gtctccccgc cgtacatgtc gggcacgtac tccacgacgt gcgcgccgag
      961 ccgcgtgaga ttgaaatgcg cgttgcggga cacgagcggg tcgaacgtcc agtaaatgcg
     1021 ggacacgccg agcttccgca ccgtgtcgcg ctggaactcc ttgagccggc gcccgatgcc
     1081 gtgatcgcgc aggtgctcgc ggaccgcgag catgtccgac cagtgcacga ggcggccgcc
     1141 ctcgacgccc gttaggccga acacgaagcc gaccagccgg ccgtcctcct cgaacgc
//
LOCUS       DASUCJ010000632         1459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_434933, whole genome shotgun sequence.
ACCESSION   DASUCJ010000632 DASUCJ010000000
VERSION     DASUCJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434933"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFU90_07540"
     CDS             <1..269
                     /locus_tag="VFU90_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU5479671.1"
                     /translation="AAAVHDIVHRHGGRYLSRSETVDTLEGTPLDTDLIALIEFPSKE
                     AVTAFATDPLYAPFAAARRSGSDSRLQLIDGNDVAGTISYLPKG"
     gene            335..610
                     /locus_tag="VFU90_07545"
     CDS             335..610
                     /locus_tag="VFU90_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU5479672.1"
                     /translation="MKRFVIERHIPGVGRLNAAQYKELAATSNAAVAKLAGQVQWMHS
                     HIAADNTFCVYLGPSEDLIREHSRIAGFPITRVNEIVTVIDPMTAFS"
     gene            complement(650..>1459)
                     /locus_tag="VFU90_07550"
     CDS             complement(650..>1459)
                     /locus_tag="VFU90_07550"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5479673.1"
                     /translation="REGMYTSCHNYGLNYGDVDKGHRANGFTTDGGFCEYQVNNINTL
                     VAITDEMSDEEATLVVTAGTAMYALTELGGLVAGESVCVTGPGPIGLLAVAVAKALGA
                     QPVILTGTRDNRLDIGRALGADSVVNVRKTPDVVAEVKKANGGKGVDYVVECSAAASA
                     VNDAVRMVNRGGKVCLAAFAHEGEVPVDVAHIVRNNIYLYGIRGEGRSATHRAEAFMR
                     QKRFDATKIHTHTFPLADLPTALRYAKDRVEDAIKVVVKANMGSPQKAAAE"
BASE COUNT          244 a          506 c          449 g          260 t
ORIGIN      
        1 atgccgcggc ggtgcacgac atcgtgcacc gccacggcgg ccggtatctc tcgcgcagcg
       61 aaaccgtcga caccctcgaa ggcacgccgc tcgacacaga cctgatcgcg ctgatcgagt
      121 ttccgtcgaa agaagcggtt acggccttcg cgaccgatcc tctctacgca ccgttcgccg
      181 ccgcgcgccg cagcggcagc gacagccgcc tgcaactgat cgacggcaac gacgtcgccg
      241 gcacgatcag ctatctgccg aaaggctgaa tgcgcgactg cgccgctcgt ccgcccgcgc
      301 agcgcgacct ctccgcaatt gaaagaacgg atccatgaaa cgctttgtca tcgaacgtca
      361 cattcccgga gtcggccgtc tcaacgccgc tcagtacaag gaactcgccg cgacatcgaa
      421 cgcggcggtc gccaagctcg cgggtcaggt ccaatggatg cactcgcaca tcgccgccga
      481 taacacgttc tgcgtctatc ttgggccaag cgaggacctg atccgcgagc actcgcgcat
      541 tgccggcttc ccgatcacgc gggtcaacga aatcgtcacc gtcatcgatc cgatgaccgc
      601 gttctcgtga cactttcggc gccgggggac gactccggcg ccggacgccc tactccgccg
      661 ccgccttttg cggagatccc atattcgcct tcaccacgac cttgattgcg tcctcgaccc
      721 ggtccttggc gtaacggagc gccgtcggca gatcggcgag cgggaaggtg tgggtgtgga
      781 tcttggtggc gtcgaagcgc ttctgccgca tgaaggcttc ggcgcggtgc gtcgcgctgc
      841 ggccctcgcc gcggatgcca tagaggtaga tgttgttgcg cacgatgtgc gcgacgtcga
      901 ccggaacctc gccctcatgg gcgaatgcgg cgaggcagac tttgccgccg cgattgacca
      961 tgcgcacggc gtcgttgacg gcgctcgccg ccgccgagca ttcgacgacg taatcgacac
     1021 ccttgccgcc attcgccttc ttcacctcgg cgacgacgtc cggcgtcttg cgcacgttga
     1081 ccacgctgtc tgcgccgagc gcgcggccga tgtcgagacg gttgtcgcgc gttccggtca
     1141 ggatcaccgg ctgcgcgccc agcgccttcg ccaccgcgac tgccaagagc ccgatcggcc
     1201 ctggcccggt gacgcaaacg ctctcgccgg cgacgaggcc gccgagctcg gtcagcgcat
     1261 acatcgccgt gccggcggtg acgacgaggg tcgcctcctc gtccgacatc tcgtcggtaa
     1321 tcgcgaccag cgtgttgatg ttgttgacct ggtattcgca gaagccgccg tcggtggtga
     1381 agccgttggc gcgatggccc ttgtcgacgt cgccgtaatt caatccgtag ttatggcaag
     1441 aagtatacat gccctcgcg
//
LOCUS       DASUCJ010000633         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_694390, whole genome shotgun sequence.
ACCESSION   DASUCJ010000633 DASUCJ010000000
VERSION     DASUCJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694390"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1161
                     /locus_tag="VFU90_07555"
     CDS             <1..>1161
                     /locus_tag="VFU90_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479674.1"
                     /translation="VAACNDVNRPDLNNASVSSFSKITSRSQVAALAVGVLSRDRANN
                     ETEITINEIIGRDAYILTTSEPRFVTQLLGTSVDPSGFLGTALWPYDGIRLANIGIDG
                     IAAVDPSTGVLTGDEINSAQGYLRTMKALLYLRALESRDTAGIPIAVDVPPTDPPAAI
                     VCKPDALRFIVALLDSADAELKAGINSPFPFSLPSGFAGFDEPGTFDRFNRALTAKVN
                     VYLAYRDFATNGSIDQTALSAAALDLDSSFVDTTNALNLNLGPVYVYTTNTGDATNGL
                     FGDPSSTTTRANPRVLTESDPGDQRVARDVVSTSTIKVAGVSSDITFDLYPSPTSTTP
                     LMLNKELILLKAEVDWGQGSLPQALALANFVRTNDGGLPAKSLSSASDILNQ"
BASE COUNT          213 a          387 c          355 g          206 t
ORIGIN      
        1 ggtcgcggcg tgcaatgacg tcaatcgccc cgacctgaac aacgcgagtg tcagcagctt
       61 ttccaagatc acgtcgaggt cgcaggtcgc ggcgctcgcc gtgggtgtgc tgagcagaga
      121 ccgggcgaac aacgaaaccg agattaccat caacgagatc atcggtcgtg acgcctatat
      181 cctcaccacg tccgaaccgc ggtttgtcac gcagttgctc ggcacgagcg tcgatcccag
      241 cggcttccta ggcacggcgc tctggccgta cgacggcatc cggctggcaa acatcggcat
      301 cgacggcatc gccgcagtcg atccatcgac cggcgtccta acgggcgatg agatcaactc
      361 ggcgcagggc taccttcgca cgatgaaggc gctgctgtac ctgcgggcgc tcgagtcgcg
      421 cgacacggct ggcatcccga tcgcggtgga cgttccgccg acggatccgc cggcggcgat
      481 cgtctgcaag ccggacgcgc tgcggttcat cgtcgcgctg ctcgattccg cggacgccga
      541 gctgaaagcc gggatcaact cgccgttccc gttctcgctg ccgtccggct ttgcggggtt
      601 cgacgagccg ggtacgttcg acaggttcaa ccgagcgctc acggcgaagg tcaacgtcta
      661 cctcgcctac cgcgattttg caacgaatgg gtcgatcgat cagaccgcgc tctcggcggc
      721 ggcgctcgac ctggattcgt cgttcgtcga cacgacgaac gcgctaaacc tgaacctcgg
      781 tccggtgtac gtctacacga ccaacaccgg cgatgcgacc aacgggttgt tcggcgatcc
      841 gtcgagtacg acgacgcgcg ccaatccgcg cgtcttgacg gaatccgacc ctggcgacca
      901 acgcgtcgcc cgcgacgtcg tttcaacgag cacgatcaag gtcgccggcg tgtcctcgga
      961 tatcacgttc gacctctatc cgtcgccgac gagcacgacg ccgctcatgc tcaacaagga
     1021 gctcattctc ctcaaggccg aggtcgattg gggacagggc agcctgccgc aggcgctcgc
     1081 actggccaac ttcgttcgga ctaacgacgg cggtctgccg gccaagtcgt tgtcttcggc
     1141 gagcgacatt ctgaatcaga t
//
LOCUS       DASUCJ010000634         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_174862, whole genome shotgun sequence.
ACCESSION   DASUCJ010000634 DASUCJ010000000
VERSION     DASUCJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174862"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..>1114
                     /locus_tag="VFU90_07560"
     CDS             369..>1114
                     /locus_tag="VFU90_07560"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479675.1"
                     /translation="MLPPTAVAFLERLLDTPGPSGFESAPARLWREEAARFATVRGDV
                     HGNSMATVEGGGAPTIMLAGHIDEIGVIVQHVDDDGYVYIAPIGGWDVQVLVGQRIRF
                     LGAQGDVFGIVGKKPIHLLRADDREKASKLTDLWVDIGSTSAKETTARLNVGDAGVID
                     SHTMTFPNNRIVSRSIDDRIGAFIVLEALRRYAEKPGAARVVATATTQEEIGYRGGGA
                     GVSAAAIGARMAIVVDVSFATDHPGIEKKEL"
BASE COUNT          184 a          355 c          402 g          173 t
ORIGIN      
        1 cgcatcggat gtcctcgggg gatgggcaga cggcgtccca atatggccgc accgcggcgc
       61 cacgacaaac gttcgggcgt ccgaattgtg tgcggcgcga tattccttcg acagtccggt
      121 atgtgggcca gggcgacgtt cggcatcggc ggtgttggct ccgccccggc gtggccgccg
      181 cgccggaggt cgccgatgcg cagcggttgg gcgtgcgggt gcggagggcg caacggcgtg
      241 ccggcccaac ggcccggcct tacggaaggc ggtgctggtc tcgcatattg ggtggttcgc
      301 acgcgccgca cgcccgcgga gttaggcggc gcataccgac cttctccgaa cggaacgacg
      361 cgatcaccat gctgcccccc accgcagtgg cctttctcga gcgattgttg gatacgcccg
      421 gcccgtcggg cttcgaatct gcacccgcgc gcctgtggcg cgaagaagcg gcgcgcttcg
      481 ccaccgtacg gggcgacgtc cacggcaaca gcatggcgac cgtcgaaggc ggcggcgccc
      541 cgaccatcat gctcgcgggc cacatcgatg agatcggcgt catcgtgcag cacgtggacg
      601 acgacgggta cgtctacatt gcgccgatcg gcgggtggga cgtgcaggtg ctcgtcggcc
      661 agcgcatccg atttctcggc gcccagggcg atgtgttcgg catcgtggga aaaaagccga
      721 tccacttgct gcgcgctgac gatcgcgaaa aagcgtccaa gctcacagac ttgtgggtcg
      781 acatcgggtc gacgagcgcg aaggagacca cggcgcgtct caacgtcggc gatgccgggg
      841 tgatcgattc gcacacgatg acgtttccga acaatcgaat cgtgtcacgg tcgatcgacg
      901 atcgcatcgg cgcgttcatc gtgctcgaag cgctgcgccg ctacgcggag aagcccggcg
      961 cggcacgcgt cgtcgcgacg gccacgacgc aggaggagat cggatatcgg ggcggcggcg
     1021 ccggcgtgag tgcggcggcg atcggcgcgc ggatggcgat cgtggtcgac gtcagcttcg
     1081 ccaccgacca cccgggcatc gagaagaagg agct
//
LOCUS       DASUCJ010000635         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_541453, whole genome shotgun sequence.
ACCESSION   DASUCJ010000635 DASUCJ010000000
VERSION     DASUCJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541453"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..>1051
                     /locus_tag="VFU90_07565"
     CDS             97..>1051
                     /locus_tag="VFU90_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5479676.1"
                     /translation="MLTVERKTIPADFEYVGQAEASKRVEVRAQVSGVIIARPYVEGT
                     DVRKGDVLFRIDPTPYEAALRSAQAQQADADARFANAERNLNRLVPLLGEHAVAQKDV
                     DDARTEEEQARAAVNNAKGAVVRAQRDYDNTFVRAEISGRAGRANLVLGALVSGPTDL
                     LTTVEQVDPIYVNFSPSDQDVLAWRRGLSDKSLIAAPGPMQVRATLADGSVYPIEGTL
                     DFADLAVQQNTGTLALRASFKNPQHTLLPGQFVRVTLLGMKRVGAILVPQRAVQQSIG
                     GSFVYVVDSANKVSTRDVKGDTFVGGQWLISSGLTAGDRVVV"
BASE COUNT          177 a          352 c          364 g          158 t
ORIGIN      
        1 cgtcgctttc ggtttggctg tctgcatccc gccgcgctcg tcaaggcggc gtgtcatcat
       61 gcgccgccgc cggcaccgcc gccgccggag gtgtcggtgc tcaccgtcga gcggaaaacc
      121 attcctgcgg atttcgagta cgttgggcaa gccgaggcct cgaagcgcgt cgaagtgcgc
      181 gcgcaggtgt cgggcgtgat catcgcgcgc ccgtacgtgg aaggcaccga tgtgcgcaaa
      241 ggcgacgtgt tgttccgcat cgacccgacg ccgtatgaag ccgccctgcg cagcgcgcag
      301 gcgcaacaag ccgacgccga cgcccgcttc gccaacgccg agcggaatct caaccgcctg
      361 gtgccgttgt tgggcgagca cgcggtcgcg cagaaggatg tggacgacgc gcgcacggaa
      421 gaagagcagg cgcgcgcggc ggtgaacaac gcaaagggcg cggtggtgcg ggcgcagcgc
      481 gactacgaca acacgttcgt ccgagccgaa atctccggac gtgccgggcg ggcgaacctc
      541 gtgctcggcg cgttagtcag tggcccaacg gacctgctca cgacggtcga gcaggtggat
      601 ccgatctacg tgaacttcag cccatcggat caggacgtgc tggcctggcg tcgagggctt
      661 tccgacaagt cgctcatcgc tgcacccggg ccgatgcagg tgcgcgcgac gctcgccgac
      721 ggcagcgtct acccgatcga aggcacgctc gacttcgccg atctggccgt ccagcagaac
      781 accggaacgc tcgcgctccg cgcgtcgttc aagaacccgc agcacacgct gctccccgga
      841 cagttcgtgc gcgtgacgct actgggcatg aagcgcgtcg gcgccatcct cgtcccgcag
      901 cgcgccgtgc agcagagcat cggcgggtcg ttcgtctacg tcgtcgactc ggccaacaag
      961 gtgagcacgc gcgacgtgaa aggcgacacg ttcgtcggcg gccagtggct gatcagcagc
     1021 ggcctgaccg ccggcgatcg cgtggtggtg g
//
LOCUS       DASUCJ010000636         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_434995, whole genome shotgun sequence.
ACCESSION   DASUCJ010000636 DASUCJ010000000
VERSION     DASUCJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFU90_07570"
     CDS             <1..277
                     /locus_tag="VFU90_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479677.1"
                     /translation="KTGTAAAFFTTAEEPDINQPTIDYVAAFVPHATGAHFNPHVTIG
                     VGTVDSLEKMLAEPFNEFTFSPAGASVYQLGDYGTARKALKLLDLKP"
     gene            274..1227
                     /locus_tag="VFU90_07575"
     CDS             274..1227
                     /locus_tag="VFU90_07575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5479678.1"
                     /translation="MSAADDPLPSWNEGAAKTAILDFVRRTTDSKDPDFVREGDRIAA
                     FDQDGTLWVEHPYYTQVAFALQRLADLAPAHPEWQTTEPYKSALAGDLAAFAKFTMND
                     LEQLVAATHAGMTTAGFHSIVVDWIAKAKHPRWDRPYTDLVYQPMLEVMQYLRANRYK
                     VYVVTGGGQAFVRAYADRVYDAQPEEIIGSSLATQFEYDDGGEAVLMRPPKLLLYDDA
                     AGKPQDIYLFLGRPPRAAFGNSTGDQQMLEYTQSGGGARLMALVLHDDDQREYAYGPD
                     SAQTDSKFGAFTQALYDKAKAKGWIVVSIKRDWKRIFAFEH"
     gene            complement(1263..2147)
                     /locus_tag="VFU90_07580"
     CDS             complement(1263..2147)
                     /locus_tag="VFU90_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU5479679.1"
                     /translation="MIVITAPTGKIGRRVLAGVLETGARVRAIVRDPGKLPDGVRDRV
                     EVVEGSHGDAEVVDRAFAGADTVFWLVPPDPRAVSVDEAYVGFTRPAAEALTRHQLER
                     VVSISALGRGSPWADKAGFVTASLAMDDLIANTGVAFRAVTNPSFMDNIARQTVSIRE
                     KGTFSLPLDGDRKMPTCAARDIAEIAARLLLDPRWDGTGYVAALGPEDLSLNDVAALM
                     SEVLDKPIRYEQISFDAYKAGFVERGVSAAMAQGLTDMAWAKNEGLDNAEPRTPENTT
                     PTSFRRWCEEELKPAVFG"
BASE COUNT          456 a          733 c          693 g          415 t
ORIGIN      
        1 taagaccggg accgccgcgg cgttctttac gacggccgag gaaccggata tcaatcagcc
       61 cacgatcgac tacgttgcgg cgttcgtccc gcatgcaacc ggcgcgcact tcaatccaca
      121 cgttacgatc ggcgtgggca ccgtagactc cctagaaaag atgctcgccg aaccttttaa
      181 cgagtttacg ttctcgccgg cgggggcgtc ggtctatcag ctgggcgact atgggactgc
      241 gcgtaaggca ctcaaacttc tggacttgaa gccatgagcg cggccgacga tccgctgccg
      301 tcgtggaacg aaggcgcagc caaaacggcg attctcgatt tcgttcgcag gacgaccgat
      361 tctaaggatc ccgatttcgt tcgcgaaggg gatcgcatcg ccgcattcga ccaggacggc
      421 acgctgtggg tagaacaccc ctactatacg caggtcgcgt tcgcgctgca gcgactcgcc
      481 gacttagctc cagcgcaccc cgagtggcag acgaccgagc cttataagtc cgccctcgcc
      541 ggcgatctag cagcgtttgc gaagttcacg atgaacgact tagagcagct ggtcgccgcc
      601 acgcatgcgg gcatgacgac ggccggcttt cactcgatcg tcgttgactg gatcgctaag
      661 gctaagcatc cgcgctggga tcgcccatat accgacctgg tttatcagcc gatgctggaa
      721 gtgatgcaat atctgcgcgc aaatcgctac aaagtgtacg tcgtcacggg cggcggccag
      781 gctttcgtgc gcgcttatgc ggaccgggta tacgacgctc aacccgaaga gatcattgga
      841 tcgtcactcg ccacgcagtt tgaatacgat gatgggggcg aggcggttct catgcgccct
      901 ccaaaactgc tgctatacga cgacgctgcc ggcaagcccc aggacattta cctcttcctc
      961 gggcggccgc cgcgagccgc cttcgggaat agtacgggcg accaacagat gttagagtat
     1021 acgcagtcag gcggcggagc gcgtttgatg gccctcgtgt tgcacgacga tgaccagcgc
     1081 gagtacgcat atggccccga tagcgcgcaa accgactcga agttcggagc gtttacccag
     1141 gcgctctatg acaaggccaa ggctaagggt tggatcgtcg tgagcataaa gcgcgactgg
     1201 aagcgtattt tcgccttcga acattaaccg cacaccctcg acaaggttcg tcgacgcgcg
     1261 gatcagccga acacggccgg cttcaattct tcctcgcacc accggcggaa gctcgtgggt
     1321 gtcgtgttct cgggcgtgcg gggctcggca ttgtcgaggc cttcgttctt cgcccaggcc
     1381 atgtcggtca aaccctgcgc catcgcagcc gacacaccgc gttcgacgaa cccggcctta
     1441 taggcgtcaa aagagatctg ctcgtagcga atgggtttgt caagcacctc ggacatgagc
     1501 gccgccacgt cgttcaacga gaggtcctcg ggcccaagcg ccgcgacata ccccgtcccg
     1561 tcccaacgag gatccagcaa caggcgagcg gcgatctccg cgatgtcccg tgccgcacag
     1621 gttggcatct tccggtcgcc gtccaacggg agcgagaacg tgcccttctc gcggatcgaa
     1681 accgtctgcc gggcgatgtt gtccatgaac gacgggttgg tcacggcacg aaaagcgacg
     1741 ccggtgttcg caatcaagtc gtccattgcg agcgacgcgg tgacaaaccc agccttgtcc
     1801 gcccaagggc tgccgcgtcc gagcgcggag atgctcacca cgcgttctag ctggtgacgc
     1861 gtcagcgctt ccgccgcggg acgcgtgaag ccgacatagg cttcatcgac ggaaacagcc
     1921 ctgggatcgg gcggaaccag ccaaaacacg gtgtctgcgc cggcgaacgc gcggtcaacg
     1981 acttcggcat cgccgtgcga accctcgacc acctcgacgc gatcgcgcac gccgtccggt
     2041 agtttgccgg gatcgcgcac gatcgcgcgg acccgcgcgc cggtttcgag cacgccggcg
     2101 agaacccgcc ggccgatttt ccccgtgggc gcggtaatga cgatcacgat ccgatcggtg
     2161 tcgcgattac agcgcccgat cccacgggcc gacctcttcg tcgtatccgc gagatcgagg
     2221 cgctgctagg caaaggttct agattcgctt cgcggccgtc acaccgagcg gaagatcgaa
     2281 acttcccgtg aacgtcg
//
LOCUS       DASUCJ010000637         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_771166, whole genome shotgun sequence.
ACCESSION   DASUCJ010000637 DASUCJ010000000
VERSION     DASUCJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771166"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFU90_07585"
     CDS             <1..676
                     /locus_tag="VFU90_07585"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5479680.1"
                     /translation="AVNVPATPPSFRHDIAATWYAPQNVVARHVSPPLSEDVYVTLKV
                     SDNLHAALLPYMWALYVDRARNDLLKTAFAQEHALLSGAGLDLRGAVQNEGLGGSAFF
                     SPDFMVAYLAWARGRPWFPALMRGLPVLGVDGTLFNIQTSSPARGRVFAKTGTWGSEN
                     LLDDDGLVTKGLAGFTTTRRGRHLAFAFYINRMAGKSSVDLRKDAAHNAGQMLGEMAA
                     DAYLLL"
     gene            693..1439
                     /locus_tag="VFU90_07590"
     CDS             693..1439
                     /locus_tag="VFU90_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEU5479681.1"
                     /translation="MKRLLVFDLDGTLAPSKSAIDSEMAGDLAALLASIKVAIISGGD
                     FPQFQTQVLARLQADGHLSNLSILPTCGTKFFTYDAGTWRKLYSEDLDAQQKTAIVAA
                     LNQAVAESGFKAEKTWGEAIEDRGSQITYSALGQQAPLDAKKVWDPDLSKRRRIKALL
                     DPMLPQFSVNIGGETSIDVTLPGIDKAYGIRKLRDILGIPLDAMLYVGDALFPGGNDY
                     PARGTGVTCIQVRDPDETKRVVETATAFLG"
     gene            complement(1444..>2898)
                     /locus_tag="VFU90_07595"
     CDS             complement(1444..>2898)
                     /locus_tag="VFU90_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU5479682.1"
                     /translation="QSAGAMTLRLHTPPHPPHPTHVVTARDRARAAAAGWRPVAAAPF
                     GTNGADTPLLMTDGTVMVHVTNATGWYALIPNNRGSYLKGKWKAMASLPSGYAPLYFS
                     SAVLPDGKLIVNGGEYNFGQGAETTLGAIYDPLQNAWTNVPPPSGWGSIGDASNVVLG
                     NGTYMLANCCSSQEALLNENTLTWTSTGSGKADTNSEEGWTLLPNGDVLTADVFGAPN
                     SELYNPGTGSWSSAGSLPANLTQGDEMGPQVLRFDGTVFVAGANSNTAIYNLTTGTWS
                     AGPQFPVGQGGQLDVADGPATLLTNGSELIAASPGLYQAPATFLKLSGTKLKIIPGPP
                     NAPNDSSYNVRLLMLPTGQVLETDNSDDVEIYTPGVQPKAGFAPSITSVPTTLSRGQT
                     YTVKGVHLNGFSQTNAYGDDATMAENYPLVRLTTSTGRVLFARTHGFSSMAIASPATV
                     TASFDVPANAPAGSAQLEVVTNGIASTPVSVTIQ"
BASE COUNT          507 a          881 c          962 g          548 t
ORIGIN      
        1 ggcggtaaac gttccggcaa ccccgccgtc cttcaggcac gatatcgcgg cgacgtggta
       61 cgcgccgcag aacgtcgtcg cgcgccacgt gtcgccgccg ttatccgaag acgtctacgt
      121 gacgttgaag gtcagcgaca acctgcacgc tgcgctatta ccgtatatgt gggcgcttta
      181 cgtcgatcgc gcgcggaacg atctgctgaa gacggcgttt gcgcaggagc acgcgctgct
      241 gagcggcgcc ggcctcgact tacgcggagc ggtgcagaac gagggcttgg gcggttcggc
      301 gttcttttcc ccggatttca tggtcgcgta tcttgcttgg gcgcgcggcc ggccttggtt
      361 ccccgcgctg atgcgcggac ttccggtcct tggggtcgac ggcacactgt tcaacatcca
      421 gacgagttcg ccggcgcgag gacgggtttt cgcgaagacc ggaacgtggg gctcggagaa
      481 cttgctcgac gacgacggac tcgtgaccaa aggactcgcg ggcttcacga cgactcgccg
      541 cgggcgtcac ttggcgttcg cgttctacat caaccgcatg gccggaaaga gcagcgtcga
      601 tctccgcaaa gacgccgcgc acaacgccgg ccaaatgctc ggcgaaatgg cggccgacgc
      661 ctatctgctg ctatgaacgc tggaaggctt caatgaaacg gttactggtc ttcgacctcg
      721 atgggacgct cgcgccgagc aagtcggcga tcgattccga gatggccggc gatttggccg
      781 ccttgctggc atcgatcaag gtcgccatca tctcaggcgg agacttcccg cagtttcaga
      841 cccaagtgct cgcacggctt caggcggacg gccatttgag caacctgtcg attttgccga
      901 cctgcgggac gaagttcttc acatacgacg cggggacgtg gcgcaagctg tattccgagg
      961 acctcgacgc gcagcagaag acggcgatcg tcgcggcgct gaaccaagcg gttgcggaat
     1021 cgggcttcaa ggcggagaaa acgtggggcg aggcgattga ggatcggggc agccaaatca
     1081 cgtattccgc attaggacag caggcgccgc tcgatgccaa gaaagtgtgg gaccccgatc
     1141 tttcgaaacg ccgccgaatc aaggctttgc tcgacccgat gctgccgcag ttttccgtaa
     1201 acatcggagg cgagacgtcg atcgacgtga cgctccccgg gatcgacaaa gcgtacggaa
     1261 ttcgtaaact ccgcgacatc ctggggatac ctctggacgc gatgctctac gtcggcgacg
     1321 cgttatttcc gggcggtaac gattatcccg cccggggaac cggcgtaacg tgcattcagg
     1381 ttcgcgatcc cgacgagacc aagcgcgtcg tcgaaaccgc gacggcgttt ctcggctaag
     1441 acgtcactga atcgttacgc tgaccggcgt cgaagcgatc ccattggtga cgacttccag
     1501 ctgcgcggag cccgccggcg cgtttgcggg tacgtcgaag ctcgccgtga cggtggctgg
     1561 ggaagcgatc gccatcgaac tgaagccgtg cgtgcgggcg aacagcacgc gaccggtcga
     1621 ggtcgtaagc cgcacgagcg gatagttctc cgccatggtc gcgtcgtcgc cgtacgcgtt
     1681 cgtctgtgaa aaaccgttga ggtgaacgcc cttcacggtg tacgtctgtc cgcgcgatag
     1741 cgtcgtcggc acgcttgtga tcgacggtgc gaatccggcc ttgggctgaa ctcccggcgt
     1801 gtaaatctcc acgtcgtcgc tgttgtcggt ttccaggact tggcccgtgg gcagcatcag
     1861 caggcgtacg ttataggagg agtcgttggg cgcgttcggc ggccccggga tgatttttaa
     1921 tttcgttccg ctgagcttga ggaacgttgc cggggcttga tacagccccg gacttgcggc
     1981 gatcagttcg ctgccgttgg tcagaagcgt cgcgggaccg tcggctacgt cgagctgccc
     2041 accttgacct accgggaact gcgggccggc gctccaggtt cccgtcgtca gattgtagat
     2101 tgcggtgttg ctgttcgcgc ccgcgacgaa cacagtgccg tcgaaccgca agacctgcgg
     2161 acccatctcg tcgccttgcg tgagattggc aggcagagag ccggccgacg accacgagcc
     2221 cgtgcccgga ttatacagct ccgagttcgg cgcgccgaag acgtcggcgg tcaacacgtc
     2281 gccgttgggg agcagcgtcc agccttcctc gctgttggtg tcggctttgc cggagccggt
     2341 cgaggtccac gtcaacgtgt tctcgttgag cagcgcctct tgggacgagc aacagttggc
     2401 gagcatgtac gttccgttgc cgagcacgac gttggaggcg tcgccgatgc tgccccaacc
     2461 ggaaggcggc ggtacgttcg tccaggcgtt ttgcagggga tcgtagatcg cgccgagcgt
     2521 cgtctcggca ccttggccga agttatattc gccgccgttg acgatcagct tcccatcggg
     2581 aaggaccgcc gaagagaagt agagcggcgc gtaacccgac ggcaaagacg ccatcgcctt
     2641 ccacttgccc ttgaggtagc tgccccgatt gttggggatc agcgcgtacc agcccgtagc
     2701 attagtcacg tgaaccataa cggtgccgtc ggtcatcagc agcggcgtgt cggcgccgtt
     2761 cgtgccgaac ggtgccgcgg cgaccggacg ccatccggcg gcggccgcgc gcgcccggtc
     2821 gcgggccgtt acaacgtgcg tcgggtgcgg cgggtggggc ggcgtgtgca gcctgagcgt
     2881 catcgcgccg gcactctg
//
LOCUS       DASUCJ010000638         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1443690, whole genome shotgun sequence.
ACCESSION   DASUCJ010000638 DASUCJ010000000
VERSION     DASUCJ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443690"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFU90_07600"
     CDS             <1..265
                     /locus_tag="VFU90_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479683.1"
                     /translation="MNATLATIPALASVGTYDEVYSHLGFYPKASIGVGTAPEFVVYD
                     ERYVSQHWTGTVVPRVRSALAAGTYGVVSDRDGVVLLRRRAAR"
     gene            complement(239..>1002)
                     /locus_tag="VFU90_07605"
     CDS             complement(239..>1002)
                     /locus_tag="VFU90_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5479684.1"
                     /translation="PLTADPRVRHAVAEAIDVPTLIAKAYRGALQSRGAGAGLFLWAY
                     DPHVYPDVPYDPAGARRLLDDAGWRIGPAGIRTKNGRRLDLLMIIQAATPGDATVADN
                     VAQYERAVGVGVTLKQYNITQFVAPASQSGPVYGGKFQLALYSFENGDDPDTTDQFAC
                     KNVPPNGYNKSRVCDPQIDALLAQGLASYDPARRKAVYRKLQAVLYRDLPIALIYRRA
                     QLNAFTTRLRGQSTSLSGAFWNVGGWSLASRAPAQ"
BASE COUNT          164 a          303 c          361 g          174 t
ORIGIN      
        1 gatgaacgca acgctagcga cgattcccgc gctagcgagc gtcggaacgt acgacgaagt
       61 ctattcgcac ctgggtttct atccgaaggc ctcaatcggc gtggggacgg ccccggagtt
      121 cgtggtctac gacgagcgct acgtctcgca acattggacc ggaacggtcg ttccgcgcgt
      181 gcgctcggcg ctggcggcgg ggacgtacgg ggtggtgtcg gatcgcgacg gcgtcgtgtt
      241 attgcgccgg cgcgcggctc gctaacgacc agccgccgac gttccagaag gctcccgaga
      301 gcgacgtgct ctgaccgcgc aggcgggtcg taaaggcgtt gagctgcgcg cgccggtaga
      361 tcagagcgat cggcaagtcg cggtagagca ccgcctgcag tttgcggtag accgctttgc
      421 ggcgcgccgg atcgtagctg gcgagcccct gcgcgagcag cgcgtcgatc tgagggtcgc
      481 aaacgcggga cttgttgtag ccgttgggcg gcacgttctt acacgcgaac tgatcggtgg
      541 tatcgggatc gtcgccgttt tcaaacgagt agagggcgag ctgaaacttg ccgccgtaga
      601 ccgggccgct ttgcgaagcc ggtgcgacga actgggtgat gttgtactgc ttgagcgtca
      661 cgccgacgcc gacggcgcgc tcgtactgcg cgacgttatc ggcgacggtc gcatcgcccg
      721 gcgtggcagc ttgaatgatc atgagcaggt cgagccggcg tccgttcttg gtacggattc
      781 cggcgggacc gatccgccag ccggcgtcgt cgagaagacg gcgcgctccc gcgggatcgt
      841 acggaacgtc gggatagacg tgcggatcgt acgcccacag gaaaaggccc gcgcccgctc
      901 cgcggctttg cagtgcgccg cgatacgctt tggcgatcag cgtcggaacg tcgatcgcct
      961 cggccacggc gtgccgcacg cgcggatcgg cggtcaacgg at
//
LOCUS       DASUCJ010000639         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1290633, whole genome shotgun sequence.
ACCESSION   DASUCJ010000639 DASUCJ010000000
VERSION     DASUCJ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290633"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFU90_07610"
     CDS             <1..1002
                     /locus_tag="VFU90_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5479685.1"
                     /translation="WLATPPDIIAQSDAYAKVIFLSMPIFFVYFVYATILRGTGDSTT
                     PFYALIVSAVLAIAITPLFIVGWFGLPKLGVVSAALAGAIANAAAFVWLLAYLARRDH
                     PLKFDRETLSDMRLDWKILGAVVRIGVPTGLQVMMVSLAELAVISFVNRFGSSATAAY
                     GAVNQVASYVQFPAISIGIAASIFGAQCIGAKREDKLGSVIRSAVGLNYAVGAVIIGA
                     CYAFAWNILGWFITDAHTLNIAHELLMITLWSYAVFGNSAVLSGIMRGSGTVLWPTAI
                     GIFAIWGVEVPAAYLLMHLVGLDGVWMGYPLAFCAGLALQFCYYEFVWKRKTHERLV"
     gene            complement(1013..1411)
                     /locus_tag="VFU90_07615"
     CDS             complement(1013..1411)
                     /locus_tag="VFU90_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEU5479686.1"
                     /translation="MATSEDASSLIRLRMSAHDAHYGGNLVDGARLLALFGDVATELL
                     IRLDGDEGLFVGYESVEFLAPVYAGDYVEAEGRIVRIGNTSRRMEFEARKVIAARPEL
                     GESAADALEPPVVVCRATGTCVTPKAKKRR"
     gene            complement(1381..2571)
                     /gene="mazG"
                     /locus_tag="VFU90_07620"
     CDS             complement(1381..2571)
                     /gene="mazG"
                     /locus_tag="VFU90_07620"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU5479687.1"
                     /translation="MLVRVVGLGPGDPGLLTIGSFEALRAVGRAVALLAPPDLSNYLR
                     DHGVEIVRDLVDDPALFVRGGNDEIERFVARLLQRDGDLGLGVLGNPLSDFPGLPPLL
                     RLLERDGVRAEIVPGMPRSTLSASIAMPLVPLPPKSSHHTWDDLVEIMARLRLGCPWD
                     REQTHRTLVPYLIEEAYEVVEAVENDEADALCEELGDLLLQIVFHSQVATESGKFTVA
                     DVVDALANKMIRRHPHVFGDAVIEDVDAQWRNWEQLKALEKTGLRRKSRLDGIPKHLG
                     ALQRGQRMQEKASRVGFDWPDVGGVLEKLDEELHELAEARREKQDDPHVREELGDVLF
                     TLANLARTLGVDAETAMRQANEKFYRRFTFMEERAAAEGKALSDLTLDELEELWQLAK
                     TQAA"
     gene            complement(2673..>2953)
                     /locus_tag="VFU90_07625"
     CDS             complement(2673..>2953)
                     /locus_tag="VFU90_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479688.1"
                     /translation="SPPVKSPFGYHIIQVEARTPGQKATLANSTERIKEMLQQQQEAP
                     LIQPFLQGLQQKAQIVVLDPRFQDLFPSPPPSAPAATTAPAASPAPTK"
BASE COUNT          453 a          947 c         1002 g          551 t
ORIGIN      
        1 tggctggcta ccccgcccga catcatcgcg cagtccgacg cgtatgcgaa ggtcatcttt
       61 ctctcgatgc cgattttttt cgtgtacttc gtgtacgcca cgattctgcg cggaaccggc
      121 gactcgacca cgccgtttta cgcgctgatc gtttccgccg tgctggcgat cgcgatcacc
      181 ccgctgttca tcgtcggctg gttcgggctt cccaagctcg gcgtcgtcag tgcggcgctg
      241 gcgggtgcga tcgccaacgc ggcggcgttc gtatggctgc tcgcgtacct ggcccggcgc
      301 gaccacccgc tgaagttcga tcgcgaaacg ctgtccgaca tgcggctgga ctggaaaatt
      361 ctcggcgccg tcgtacggat cggcgtgccg acggggctgc aggtcatgat ggtttcgctc
      421 gcggagctcg cggtgatctc gttcgtgaac cggttcgggt cgagcgcgac cgcggcgtac
      481 ggggcggtca atcaagtcgc cagctacgtc cagtttccgg ccatctcgat cggcatcgcc
      541 gcctcgatct tcggcgcgca gtgcatcggc gcaaaacgcg aggacaagct cggcagcgtc
      601 atccgctcgg ccgtcggcct gaactacgcc gtcggggcgg tcatcatcgg ggcgtgctac
      661 gcgttcgcct ggaacatcct gggctggttc attaccgatg cgcacaccct gaacatcgcg
      721 cacgagctgc tgatgatcac gctgtggagc tacgcggtct tcggaaactc cgccgtgctc
      781 agcggaatca tgcgcggcag cgggacggtc ctctggccga cggcgatcgg aattttcgcg
      841 atttggggcg tcgaagttcc ggccgcgtac ctgctgatgc acctggtcgg tttggacggc
      901 gtatggatgg gctacccgct ggcgttctgc gcgggcctcg cgctgcagtt ctgctattac
      961 gagtttgtgt ggaaacgcaa gacgcacgag cgcctggttt aagccgggcg ggttagcgcc
     1021 gcttctttgc cttgggcgtg acgcaggtac cggtagcccg gcagaccacc accggcggct
     1081 ccagcgcgtc ggcggcgctt tcgccgagct cgggccgggc ggcgataact ttgcgcgcct
     1141 cgaactccat gcggcgcgag gtgttgccga tgcgtacgat ccggccctcc gcttcgacgt
     1201 agtcgccggc gtagacgggc gccagaaact cgaccgactc gtaaccgacg aatagcccct
     1261 cgtcgccgtc gagacggatc aagagctcgg tggcgacgtc gccgaacagc gccagcaggc
     1321 gggcgccgtc gacgaggttg ccgccgtagt gcgcgtcgtg cgcgctcatg cgcagccgga
     1381 tcaggctgct tgcgtcttcg ctagttgcca cagttcctcg agttcgtcga gcgtgagatc
     1441 ggaaagcgcc ttcccttcgg cggcggcgcg ctcttccatg aaggtaaacc gccggtagaa
     1501 cttttcgttg gcctgacgca tcgccgtttc ggcgtcgacg ccgagcgtcc gcgcgagatt
     1561 cgccagcgtg aaaagcacgt cgccgagctc ctcgcggacg tgcgggtcgt cctgcttctc
     1621 gcgccgcgct tcggcgagct cgtgcagctc ctcgtcgagc ttttcgagaa cgccgccgac
     1681 gtcgggccag tcgaagccga cgcgcgacgc cttttcctgc attcgctgtc cgcgctgcag
     1741 cgcgccgaga tgcttgggga tcccgtcgag ccggctcttg cgccgcaaac ccgttttttc
     1801 caacgctttg agctgctccc agttgcgcca ctgcgcgtcg acgtcttcga tgacggcgtc
     1861 gccgaagacg tgcgggtgcc ggcgaatcat tttattggcg agcgcgtcga ccacgtcggc
     1921 caccgtaaac ttgccggact cggtggcgac ctgcgagtga aagacaattt gcagcagcag
     1981 atcgccgagc tcttcgcaga gtgcgtcggc ctcgtcgttc tcgacggcct cgacgacctc
     2041 gtacgcctcc tcgatcaagt acggaaccag cgtgcggtgc gtttgctcgc ggtcccacgg
     2101 gcagccgagc cgcagccgcg ccatgatctc gactaaatcg tcccaggtat ggtgcgacga
     2161 ttttggcggc aggggcacga gcggcatggc gatcgaggcg gagagcgtgg agcgcggcat
     2221 tccgggaacg atctcggcgc gtacgccgtc gcgttcgagc aagcgcagga gcggcggaag
     2281 gcccgggaag tcggagagcg ggttgccgag aacgcccagg ccgagatcgc cgtcgcgctg
     2341 cagcaaacgc gcgacgaagc gttcgatttc gtcgttgccg ccgcgcacga agagagccgg
     2401 atcgtcgacg agatcgcgca cgatctcgac gccgtggtcg cgcaggtagt tgctcagatc
     2461 gggcggtgcc agcaatgcga cggcgcgccc gaccgcgcgc agggcttcga agctcccgat
     2521 cgtcaggagg cccggatcgc cgggacccaa tcctacgacg cggaccagca tggcagcgcg
     2581 atttccagca gggcgcggaa aacccgcctg acggatgccg aaaggccccg gcgcgtgccg
     2641 gggcctgtcg ggtgcgtcgt tgcgaaacgc gtttacttag tcggagcggg cgatgccgcc
     2701 ggcgccgtcg ttgccgccgg cgcggagggc ggcggcgacg ggaacagatc ctggaagcga
     2761 ggatcgagca cgacgatctg cgccttctgc tgcaggcctt ggaggaacgg ctggatcagc
     2821 ggcgcctcct gctgctgctg gagcatctcc ttgattcgct cggtggagtt ggccagcgta
     2881 gccttttgac cgggcgttcg cgcttcgact tgaatgatgt ggtagccgaa cggcgatttc
     2941 accggcggac tga
//
LOCUS       DASUCJ010000640         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1504663, whole genome shotgun sequence.
ACCESSION   DASUCJ010000640 DASUCJ010000000
VERSION     DASUCJ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504663"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFU90_07630"
     CDS             <1..699
                     /locus_tag="VFU90_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5479689.1"
                     /translation="IVMPADAPEVKLAATRGYGAEIVPYERARADRAEIARDIAASLG
                     ATLVPPFDDPRIVAGAGTAALELLEEAGRLDAIVVPVGGGGLLSGTAIAAHAVDPSTA
                     IYGVEPEAGDDFARSLASGELVRTEVPQTIADGLQTISPGELTLRIAREHAARIVTVS
                     DAELVDAMRFAFERMKLIVEPSAAAPIAALLRQRLPEVAGKRVGVVVSGGNVDAARYA
                     RLLSEAGDGLRPIE"
     gene            complement(648..1265)
                     /locus_tag="VFU90_07635"
     CDS             complement(648..1265)
                     /locus_tag="VFU90_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEU5479690.1"
                     /translation="MRSRPARPSPASRFGEPDEAELPADIRDVYAANRSKIGFVPNVF
                     RAYARRPEHFRAFMAYHDVLMKGAGGLSRAEREAIVVAVSSENACQYCLTAHGAALRI
                     LGKDPILAEQIANNWRTADLSPRWRAMLAFASRVNEPGFAATDDEIAALRASGFSDDD
                     VWDIAAIAAFFGFSNRMAGAMDMRPNAEFYSMGRNPSPASESKRA"
     gene            complement(1246..1662)
                     /locus_tag="VFU90_07640"
     CDS             complement(1246..1662)
                     /locus_tag="VFU90_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU5479691.1"
                     /translation="MKTFRLVLAALALAAGCTIGAAGPTSAATSSLTVDLGAQNASGE
                     TGTAALTQKPEGVEVVITLANAPATAQPAHIHQGTCGQLGGVAYPLTSVTGGNSTTIV
                     KGVTIDQLLASPMAINVHKSTSDLATYVACGEIKAS"
     gene            complement(1720..4662)
                     /locus_tag="VFU90_07645"
     CDS             complement(1720..4662)
                     /locus_tag="VFU90_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479692.1"
                     /translation="MPRIATPFVLIALLGVSPALYHDMHWRFVGPLRGGRTTSIAGVP
                     NRPNLFYIGVVNGGIWKTTDAGRTWTPIFDAEPTGSIGALTIAPGAPNVIYAGSGEGL
                     QRPDLSIGDGIYKSTDGGTSWTHLGLRDGQQIAAIAVDPADANRLFVAVLGHPFGPNG
                     ERGIYRSIDGGTSFARVLYKGPDAGAYDVVLDPHDSNTLYATLWAARQAPWEIGGSFE
                     IPGSGIFKSTDGGSTWTQLTAGLPARIGRAEVAVAAGDPRVVYAYADTEDLGGAVYRS
                     TDGGAHFTRTNADPEIAQRGDDLVSIAVDPRDAGVVYLTNTSFYRSSDGGRTFVAIKG
                     APGGDDYHAVWLDPNDANTIAVASDQGAAISVNHGATWSSWYNQPTAQMYHASADGRF
                     PYWICGGQQESGSACVSSRGNWGQITQRDWHTAGAQEYGYVVSDPLHPGVFFGGKVER
                     FDERTGQAQEVSPIALRSVGYRTVRTEPLAFDNFDRRRLYFGANVVFQTDDGGMRWRV
                     ISPDLTRARPGVPPVLGTFESDDPQRGRHRGVVYALAPSYRSPGSLWAGTDDGLVWVT
                     HDGGRHWRNVTPPGLSPWSKVAQIDASHFDGGTAFVAVNRFRLDDLHPYAYVTRDGGA
                     RWKSIVAGLPVQPVNAVRQDSQEPNLLYAATENGVFVSFDAGAQWQSLQLDLPHTSVR
                     DVIVHGNDLIVATHGRGFWILDDVTPLRELAHDGGDPFAVSHRSAYLFPPERAYRVRD
                     NVNTDTPLPPEEPAGENPPNGAILDYALREGARRVTLTIYDASGRIVRRYASDDAPPP
                     PIPNLDKPAYWERPFRRPATGAGMHRFVWDLREAPPQATEQGLPMAAVAHDTPRVTLG
                     PLVVPGRYLVELDADGMKTRRVLAVTMDPRIAISAASLERQYRLAQRLAALMDRSYRD
                     AAAAKAAGRSAASAPFDRLNGDAASLLDAVDGADAAPTYAATAAVATLEARLRDAEQP
                     LAQPRRNP"
     gene            complement(4678..>5512)
                     /locus_tag="VFU90_07650"
     CDS             complement(4678..>5512)
                     /locus_tag="VFU90_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5479693.1"
                     /translation="VRKISGPNLLRNYRRAIGRGALFFGRLPRGVARVCVTERTAGVR
                     GEWVFRNADPDARVVMYYLHGGGYVAGSPATYRTLTGAFARRCATRVFALDYRLAPEH
                     PFPAPVHDAVTGYRWLLAAGIAPRDIVVAGDSAGGGLAVSMLVTLRDEGTPLPAAAVL
                     IAPWVDLAAERNLVAADRRERQIAQAYVGERSLDHPLASSLYAELHDLPPLLIQASTI
                     EMLRDDAVRLDAKARAAGVDSKLRLWDGVPHVWHIFAGLRESREAFGDIAAFVARVTQ
                     P"
BASE COUNT          872 a         1838 c         1956 g          846 t
ORIGIN      
        1 atcgtcatgc cggctgacgc accggaagtc aaactcgccg ccacccgcgg ctacggcgcg
       61 gagatcgtgc cgtacgagcg cgcgcgcgcc gaccgcgcgg agatcgcgcg cgacatcgcc
      121 gccagccttg gggcgacgct ggtgcctccg ttcgacgatc cgcgcatcgt cgcgggcgcc
      181 ggtacggccg ccctcgagct gctggaagaa gcgggtcggc tcgacgcgat cgtcgtgccg
      241 gtcggcggcg gcgggctgct gtcgggcacg gcgatcgccg cacacgcggt cgatccgtcg
      301 acggcaattt acggcgtcga gcccgaggcc ggcgacgact tcgcgcgctc gctcgcatcc
      361 ggcgaactcg tccggacgga ggtcccgcag acgatcgccg acggcttgca aacgatcagc
      421 ccgggcgagc tgaccctacg gatcgcgcgc gagcacgccg cccggatcgt tacggtgagc
      481 gatgccgagc tggtcgatgc gatgcggttt gcgttcgaac ggatgaaatt gatcgtcgag
      541 cccagcgccg ccgcgccgat tgccgcgctg ctgcggcaac gcctccccga ggtagccggg
      601 aaacgcgtcg gcgtcgtggt gagcggcgga aacgtcgacg ccgcgcgcta cgcgcgcttg
      661 ctttccgaag ccggcgacgg gttgcggccc atcgagtaga actcggcgtt gggacgcatg
      721 tccatcgcgc cggccatccg gttggagaaa ccgaagaaag cggcgatcgc cgcgatgtcc
      781 caaacgtcgt cgtccgagaa accgctggca cgcagcgcgg cgatctcgtc gtcggttgcg
      841 gcgaaccccg gctcgttgac gcgcgacgca aacgccagca tcgcgcgcca tcgaggggag
      901 agatcggcgg tacgccagtt attggcgatc tgctcggcca aaatcggatc cttgccgaga
      961 atccgcaagg ccgcaccgtg cgcggtcagg cagtactggc acgcattctc cgacgaaacc
     1021 gcgaccacga tcgcctcgcg ttcggcgcgg ctcaatccgc cggcgccttt catcaagacg
     1081 tcgtgatagg ccatgaacgc ccggaagtgc tccggccgcc gcgcgtacgc tcggaaaacg
     1141 ttcggcacga agccgatctt cgaccggttc gccgcgtaga cgtcgcgaat gtcggccgga
     1201 agctcggcct cgtcgggttc tccgaaacgc gaggcgggcg acggcctagc tggccttgat
     1261 ctcaccgcag gcgacgtacg ttgcgagatc cgaagtgctc ttgtgcacgt tgatcgccat
     1321 cggcgacgcg agcagctgat cgatcgtgac gcccttgacg atcgtcgtgg agtttccgcc
     1381 ggtgacgctc gtgagcggat acgcgacgcc gccaagttgg ccgcacgtgc cctgatggat
     1441 atgggcgggc tgcgcggtcg ccggcgcgtt ggccagcgtg atcacgactt cgacgccttc
     1501 gggtttttgc gtcagcgccg ccgtgccggt ctctccggaa gcattttgcg caccgaggtc
     1561 gacggtcagc gaactggtcg ccgccgacgt cggtccggcg gccccgatgg tgcatccggc
     1621 ggcgagcgcg agcgcagcca agacgagacg gaaggttttc atggacgaac tgctcctttc
     1681 gtgaagaacg cctacgttat acccagcggt ggagcgggcc taggggtttc gccgaggctg
     1741 ggcgagaggc tgctcggcgt cccggagccg ggcctcgagg gttgcgaccg ccgcggtcgc
     1801 cgcgtaggtc ggcgcggcgt ccgcaccgtc gaccgcgtcg agcagggacg ccgcgtcgcc
     1861 gttgaggcga tcgaacggtg ccgacgcggc actccgcccg gcggctttgg cggccgccgc
     1921 gtcgcgatag ctgcggtcca tcagcgcggc gagccgctgc gcgagccggt attgccgctc
     1981 cagcgacgcc gcggagatcg cgatgcgcgg gtccatggtc accgccagca ctcgccgcgt
     2041 tttcatcccg tcggcatcga gctcgaccag gtagcggccg ggaaccacca gcggacctag
     2101 cgtaacgcgg ggagtgtcgt gcgcgaccgc cgccatcgga aggccctgct ccgtggcctg
     2161 tggcggcgcc tcgcggagat cccaaacgaa ccggtgcatg cccgcgccgg tcgccggccg
     2221 gcggaacggg cgctcccaat aggccggttt gtcgagattt gggatcggcg gcggcggcgc
     2281 gtcgtcgctg gcgtatcggc ggacgatccg tccgctcgca tcgtagatcg tcaaggtgac
     2341 gcggcgcgcg ccctcgcgga gcgcgtaatc gagaatcgca ccgttgggcg gattctcgcc
     2401 ggccggttct tcgggcggca agggcgtgtc ggtgttgacg ttatcgcgca cgcggtacgc
     2461 gcgttcggga ggaaacagat acgcgctgcg atgcgaaacc gcaaacggat cgccgccgtc
     2521 gtgcgcgagt tcgcgcaggg gcgtgacgtc gtcgaggatc caaaagccgc ggccgtgggt
     2581 ggcgacgatg aggtcgttgc cgtggacgat cacatcgcgt accgacgtgt gcggcagatc
     2641 gagctggagc gactgccact gcgcgcccgc atcgaacgag acgaaaacgc cgttttcggt
     2701 ggcggcgtac agcaggttgg gctcctgcga gtcctgacgc acggcgttga cgggttgaac
     2761 cggcaagccc gcgacgatcg acttccagcg cgcgccgccg tcgcgggtga cgtacgcgta
     2821 cgggtgcaga tcgtcgaggc ggaaacggtt caccgcgacg aacgcggtgc cgccgtcgaa
     2881 atgcgacgcg tcgatctgag cgactttact ccacggcgaa aggccgggcg gggtgacgtt
     2941 tcgccagtga cgcccgccgt cgtgcgtgac ccagacgagc ccgtcgtcgg tgcccgccca
     3001 cagcgacccc gggctgcggt acgaaggtgc gagcgcgtac acgacgcctc gatgccggcc
     3061 gcgttgggga tcgtcgctct cgaacgtacc cagcaccggc gggacgcccg gacgcgcgcg
     3121 cgttagatcc gggctgatca cgcgccagcg catcccgccg tcgtcggtct ggaagacgac
     3181 gttcgcgccg aagtacaaac ggcgccggtc gaagttgtcg aaggcgagcg gttcggtgcg
     3241 aacggtgcga tagcccaccg agcgcagcgc tatcggagaa acctcttgtg cttgtccggt
     3301 gcgctcgtcg aatcgctcga ccttcccgcc gaagaacacg ccggggtgga gcgggtcgga
     3361 aacgacgtag ccgtattcct gcgcgccggc ggtatgccag tcgcgctgcg taatctgccc
     3421 ccaattgccg cggctgctga cgcacgccga gccgctttcc tgctgcccgc cgcaaatcca
     3481 atacggaaac cggccgtccg cggaagcgtg gtacatctgc gcggtcggct gattgtacca
     3541 agaactccac gtggctccgt gattgaccga aatcgccgcg ccctgatcgc tcgcgacggc
     3601 gatcgtgttg gcgtcgttcg gatcgagcca aacggcgtgg tagtcgtcgc cgccgggcgc
     3661 gcccttgatg gcgacgaacg ttcgaccgcc gtcgctcgaa cggtagaacg acgtgttcgt
     3721 cagatagacg acgccggcat cccgcggatc gaccgcgatc gaaacgaggt cgtcgccgcg
     3781 ctgcgcgatc tccggatccg cgttggtgcg cgtgaagtgc gcgccgccgt cggtgctgcg
     3841 atagaccgcg ccgccgagat cctcggtatc ggcgtaggcg tagacgacgc gcgggtcgcc
     3901 ggctgccacc gcgacttcgg cgcgtccgat tcgagccggc aggccggcgg tgagctgcgt
     3961 ccaggtactc ccgccgtcgg tcgacttgaa aatgccgctt ccgggaattt cgaacgagcc
     4021 gccgatttcc cagggcgctt gccgagccgc ccacaaggtc gcgtagagcg tgttggaatc
     4081 gtgcggatcg agaacgacgt cgtacgcgcc ggcatccggt cctttataga gcacgcgcgc
     4141 aaagctcgtg ccgccgtcga tcgagcgata gattccgcgt tcgccgttgg gtccgaacgg
     4201 gtggccgagg accgcgacga acagccggtt agcgtcggcc ggatcgacgg cgatggccgc
     4261 aatctgctgg ccgtcgcgca ggccgaggtg cgtccacgac gttccgccgt cggtggattt
     4321 gtagatgccg tcgccgatcg agaggtccgg ccgctgcagc ccttcaccgc tgcccgcata
     4381 gatgacgttg ggggcgcccg gcgcgattgt gagcgcgccg atcgaacccg taggctcggc
     4441 gtcgaagatc ggcgtccacg tccgccctgc atcggtcgtc ttccaaattc cgccgttgac
     4501 caccccgata tagaagaggt tcgggcggtt cggaacgccg gcgatcgagg tcgttcgtcc
     4561 gccgcgcaac ggcccgacga accgccagtg catgtcgtgg tagagcgcgg gcgagacgcc
     4621 cagcagagcg atcagcacaa acggcgtcgc gatgcgcggc atacggaatc gatctcccta
     4681 cggttgcgtg acgcgggcga cgaaggcggc gatgtcgccg aacgcttcgc ggctttcgcg
     4741 caggcccgcg aaaatatgcc agacgtgcgg aaccccgtcc cacaggcgca gcttcgaatc
     4801 gacgcccgcg gcgcgagctt tcgcgtcgag ccggaccgcg tcgtcgcgca gcatctcgat
     4861 cgtgctcgcc tgaatcagca gcggcggcag gtcgtgcagc tcggcgtaca ggctcgatgc
     4921 gagcggatga tcgagcgatc gctcgccgac gtacgcttgc gcgatctggc gctcgcggcg
     4981 atccgcggcg accaggttgc gctcggcggc gagatcgacc cagggcgcga tcagcaccgc
     5041 agcggcaggc agcggcgtgc cttcgtcgcg cagcgtcacc agcatcgata cggcgagccc
     5101 gccgccggcc gagtcgccgg cgacgacgat gtcgcggggt gcgattccgg ccgcgagcag
     5161 ccagcggtag ccggtcacgg cgtcgtgtac gggggccgga aaggggtgct ccggcgccag
     5221 ccggtagtcc agcgcgaaga cgcgcgtcgc acagcgccgg gcgaacgcgc cggtgagcgt
     5281 gcggtacgtg gcgggggagc ccgccacgta cccgccgccg tgcaggtagt acatgacgac
     5341 gcgcgcgtcg ggatcggcgt tgcgaaacac ccactcgccg cgcacgccgg cggttcgctc
     5401 ggtgacgcac acgcgagcca cgccgcgcgg aagccggccg aaaaagagcg cgccgcggcc
     5461 gatcgctcgc cggtagttgc gcagcaagtt cggtccgctg atctttcgca cc
//
LOCUS       DASUCJ010000641         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_403802, whole genome shotgun sequence.
ACCESSION   DASUCJ010000641 DASUCJ010000000
VERSION     DASUCJ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403802"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..906)
                     /locus_tag="VFU90_07655"
     CDS             complement(199..906)
                     /locus_tag="VFU90_07655"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5479694.1"
                     /translation="MRKSIQSILGLTTVALAIAACGNGNNEPPKSPDNPPGTTAPAPA
                     TGSAMDAGTTMLTPPATPAPVGTSSSEMASTERMASEPSTSPPTDDQILQIVHVANEG
                     EIEQAKLAQSKAKDPRVKKFAAMMQKDHTAADDKGLSMGRRDHIDLSPSKASSDLESG
                     ANDNTQMLKGASGADFDKSYVDTQVKEHQEVLDAIDQKLVPNAKNADLRTYLAEVRTK
                     VAMHLQHAKDLQAQLAK"
BASE COUNT          183 a          395 c          371 g          242 t
ORIGIN      
        1 acttaagcgg attcgccata aaccaaggca tatcacgcgc tcgcgcgaca ccgcacgggc
       61 gaatccacca gccgtcgttc tttccagccc gaccgtagag gccgtcgcgc aagacgccgc
      121 tgcgcgggcg tctcggccca cagcggcgct cgtggaacga cggcggtcct agcgcgcgca
      181 ctcggtgatg ctcgcgcgct acttcgccag ttgcgcctgc aggtctttgg catgctgcag
      241 gtgcatcgca accttcgtcc gtacttcggc gagatacgtc cgcaggtctg cgttcttcgc
      301 gttgggcacg agcttctggt caatcgcgtc gagcacctct tggtgctctt tgacttgcgt
      361 gtcgacatag ctcttgtcga agtccgcgcc cgatgccccc ttcaacatct gggtattgtc
      421 gttcgcgccg gactcgagat ccgagctcgc tttgctcggc gataggtcga tgtgatcgcg
      481 ccggcccatc gaaagaccct tgtcgtccgc cgccgtgtgg tccttctgca tcatcgcggc
      541 gaacttcttc actcgggggt ccttagcctt cgactgcgcc aatttcgctt gttcgatttc
      601 cccctcgttg gccacgtgca cgatctggag aatctgatcg tcggtcggag gcgacgtcga
      661 cggctccgaa gccatccgct cggtcgaggc catttcgctc gacgacgtcc cgaccggcgc
      721 cggcgtcgcc ggcggggtca gcatcgtcgt cccagcgtcc atcgcagagc ccgtagcggg
      781 cgccggcgcc gtggtgcccg ggggattgtc cggcgacttc ggcggttcgt tgttgccatt
      841 gccgcaagcg gcgatagcga gtgcgaccgt ggtcaaaccg agaatgcttt gaattgattt
      901 tctcatggcg gacgcgccac acagcaatct ggatgccagc gagcaacggg tctctttccg
      961 ctcgaaggca cgggagcgac gcggtcacca cagccatttc gccgcgacga ggcgagcgcg
     1021 cgacacggac gcacaccatt cggaggtccg gcgctgtcgc attccgccgg ctgcaccgtc
     1081 gccgaacgca gcggtgagcc ggaggcaagg ctcggcggag gacctagagc gccgctcaat
     1141 cgattggaac acttctcgtt cggcgctcta gtttctcggg tggatcgctc c
//
LOCUS       DASUCJ010000642         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_465530, whole genome shotgun sequence.
ACCESSION   DASUCJ010000642 DASUCJ010000000
VERSION     DASUCJ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465530"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFU90_07660"
     CDS             <1..396
                     /locus_tag="VFU90_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU5479695.1"
                     /translation="SAIINGDRTGAGFGSGGIWEDANAYTFPDWVQIDFAGTKTLDHV
                     IVYSMQDSYGQPVEPTDTMTFHSYGVIDFMVQGWDGSQWVTLGTVTGNDLVKRTVTFP
                     AFTTDRIRVVITNAENGYSRLAEVEAFGH"
     gene            552..>1759
                     /locus_tag="VFU90_07665"
     CDS             552..>1759
                     /locus_tag="VFU90_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479696.1"
                     /translation="MTNMSSFLDAPVGRRVDPHELPESSMRIFAAVSRPASQLCRLLT
                     LAALALASAAAWAAPGALVSKTLTQIPGNDTKSYAIALQSDGKILTAGYAYLGTKQFA
                     ITRYNADGTVDTGFADAGTRLEPLGATNSEAHAIGVQSTGKIVVGGFAVGSSSQFGIA
                     RFNTDGSLDTSFGGGGALTSVGTDSGALAMAIQSDDKIVLAGWSTVSGTKVFALARYD
                     ASSPTLDSTFGSGGVVIGSADALDEQINGVALQADGKIVAVGSYGSGNSNETTVRRYN
                     ADGSLDTSFGTSGTQFLTLMQDGHAVAVLANGSILVAGEYGGAFTVARLTSAGTLDTT
                     FGVNGYAQQGDGSSSGLGKAMQVQQDGKIVVAGTGGNGSASAIAIARFNADGSNDTSF
                     GNSGSTIVQF"
BASE COUNT          316 a          636 c          559 g          248 t
ORIGIN      
        1 tcggcgatca tcaacggcga tcgcaccggc gcaggcttcg gctcgggcgg catctgggaa
       61 gacgccaacg cctacacctt cccggactgg gtgcagatcg acttcgccgg cacgaagacc
      121 ctcgaccacg tgatcgtcta ctcgatgcag gacagctacg gccagccggt cgagcccacc
      181 gacaccatga ccttccacag ctacggcgtg atcgacttca tggtgcaggg ctgggacggc
      241 agccagtggg tgaccttggg cacggtcacg ggcaacgacc tcgtgaagcg caccgtcacc
      301 ttccccgcgt tcacgaccga tcgcatccga gtcgtgatca ccaacgccga gaacggctac
      361 tcgcgcctcg cggaggtgga agcgttcggc cactgaagca cagcgacaac gcagcaaccg
      421 acaaaggccg gcctcgtgaa atcggggccg gttctttttt tgcgggaaat cgcgacgccg
      481 atttcctaca tcgcttgcca gcgccgaccg acaaaacgtg gcgcgacggc cgacagaaaa
      541 acaagcgcgg catgacgaac atgtcgtcat tcctcgacgc gcccgtcgga cgtcgggtcg
      601 atccacacga actgccggag tcgtccatgc gcattttcgc cgccgtttct cgccccgctt
      661 cgcagctctg ccgcctgctc accctcgccg cgctcgcgct cgcgtccgcg gccgcctggg
      721 ccgcgcccgg cgcgctggtc tcgaagacgc tcacccagat tcccggcaac gacaccaagt
      781 cgtacgcgat cgcgctgcag tccgatggca agatcctcac cgcgggatac gcgtacctag
      841 gcacgaagca gttcgcgatc acgcgctaca acgcggacgg cacggtcgac acgggcttcg
      901 ccgacgcggg cacccgcctc gagccgctcg gcgcgaccaa ctccgaagcg cacgcgatcg
      961 gcgtgcaatc gaccggcaag atcgtggtgg gcggcttcgc ggtgggcagc agcagccagt
     1021 tcggcatcgc gcgcttcaac accgacggca gcctcgacac ctcgttcggc ggcggcggag
     1081 cgctcaccag cgtgggcacg gactccggcg cgctcgccat ggcgatccag tccgacgaca
     1141 agatcgtcct cgccggctgg tcgaccgtga gcgggaccaa ggtcttcgcc ctcgcgcgct
     1201 acgacgcgag ctcgccgacg ctcgacagca cgttcggctc gggcggcgtg gtcatcggca
     1261 gcgccgacgc gctcgacgag cagatcaacg gcgtggccct ccaggccgac ggcaagatcg
     1321 tcgcggtggg ctcctatggg agcggcaaca gcaacgagac gaccgtgcgc cgctacaacg
     1381 cggacgggag cctcgacacc tccttcggta ccagcggcac ccagttcctc acgctcatgc
     1441 aggacggcca cgcggtcgcc gtcctcgcga acggcagcat cctcgtcgcc ggcgagtacg
     1501 gcggcgcctt cacggtcgcg cgcctcacct cggcgggcac gctcgacacg acgttcggcg
     1561 tgaacggata cgcgcagcaa ggcgacggca gctcgtccgg cctcggcaag gccatgcagg
     1621 tgcagcagga cggcaagatc gtcgtcgcgg gcaccggcgg caacggctcc gcctcggcga
     1681 tcgcgatcgc gcgcttcaac gccgacggct cgaacgacac gagcttcggc aacagcggct
     1741 cgacgatcgt ccagttcag
//
LOCUS       DASUCJ010000643         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_969879, whole genome shotgun sequence.
ACCESSION   DASUCJ010000643 DASUCJ010000000
VERSION     DASUCJ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969879"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFU90_07670"
     CDS             complement(<1..1034)
                     /locus_tag="VFU90_07670"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5479697.1"
                     /translation="MSASTHLSVDRPGASRAYRTCLQIAIVLGLVAIVHAALDGTVAR
                     LIGRGLEARGLDPSLLRPGVIWVSLGVLMLVGRTVLWFAYRPRRPAEFGAAPTMTVVI
                     PAYNEGPTVERAIDSVAGACYPAERLQIIVVDDGSTDDTWDWIVRAAARHSGRVTTLR
                     LSHNSGKREALAAGFRVARGAILVTVDSDSVIEHDALLAMAGPFRDPRIGAVAGKVAA
                     WNRDRGAISRMLHVRFILSFDVQRSVQSTCRTVYCCPGALAAYRASVVKRVLEPWLSQ
                     RFLGARCTFGEDRALTNDIMACGFDTVYQSNAVVQTLVPTTYAGLCRMYLRWDRSHVR
                     EELRFLRIVW"
     gene            1216..1959
                     /locus_tag="VFU90_07675"
     CDS             1216..1959
                     /locus_tag="VFU90_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5479698.1"
                     /translation="MTHQPRNEPPARAGTILVVDDDAEIRSLVAEYLGQQGYEVVVAR
                     DGTAMFTALDTRRPDLVVLDVMLPGEDGLALCRRLRAQSDLPVIMLTARGDDIDRIIG
                     IEMGADDYLAKPFNPRELLARIRSVLRRVRAPPAAAGQVRQLRFAGWTLDCVARHLVD
                     ADGVVVPMSGAQFRLLMAFVDRPQRVLDRDQLLDATRGITADAFDRSIDVQISRLRQM
                     LRDNGRRPRIIKTVRNGGYVLAATVERIG"
     gene            1959..>2378
                     /locus_tag="VFU90_07680"
     CDS             1959..>2378
                     /locus_tag="VFU90_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479699.1"
                     /translation="MRLVPRSLFGRTLLVLVAGLVLAQVASQTLNFFDRGSGVYRLAA
                     QQTALRIARAARVLNRVPPHERQAIVDEMNGGSFSVALANAPVGMAVGFAGYDRYERE
                     LSALIRHYLRADWRTSVVITSLGRGERPRNYEAVDTTP"
BASE COUNT          398 a          794 c          806 g          380 t
ORIGIN      
        1 ttccatacga tccgcaggaa gcgcaattcc tcacgcacat ggctgcgatc ccagcgcagg
       61 tacatcctgc acagtcctgc gtaagtcgtc ggcaccagcg tctgcacgac cgcgttgctc
      121 tggtagacgg tgtcgaagcc acacgccatg atgtcgttgg tcagcgcgcg gtcctcaccg
      181 aacgtgcatc gtgcaccgag gaagcgttgc gaaagccacg gctcgagaac cctcttcacg
      241 acacttgccc ggtaggcggc gagcgccccc gggcagcagt agacggtccg acacgtcgac
      301 tgcacgctgc gctggacgtc gaaggagaga atgaatcgta cgtggagcat ccgcgagatt
      361 gcgccgcgat cgcgattcca ggccgccacc ttgccggcga cggcgccgat gcgcggatcg
      421 cggaacggtc ccgccatcgc gagcagcgca tcatgctcga tcacggagtc cgaatcgacc
      481 gtcaccagga tcgccccgcg ggcgacgcgg aatccagcgg caagcgcttc gcgcttgccg
      541 ctgttgtgcg acagccgcag cgtcgtcacc cgacccgaat ggcgggccgc agcgcgcacg
      601 atccagtccc aggtgtcgtc ggtgctcccg tcgtcgacga cgatgatctg caggcgttcg
      661 gccggatagc aggcaccggc gaccgagtcg atcgcccgct cgaccgtcgg gccttcgttg
      721 tacgccggga tcacgaccgt catcgtcggc gcggcgccga actcggcggg ccgtcgcggg
      781 cggtaggcga accagaggac ggttcgtccg accagcatca ggactccgag cgaaacccag
      841 atgacgccgg gtcggaggag cgacggatcg aggccccgcg cctcgagtcc cctgccgatc
      901 aggcgcgcga cggtgccgtc gagcgccgcg tggacgatcg cgacgagtcc gagcacgatg
      961 gcgatctgca ggcaggttcg gtacgcgcgg gacgctcccg gacgatcgac ggaaaggtgt
     1021 gtggaagcgg acatcggcgg ggcacaggtg gagccgtcat ggcgattaca actcgccagt
     1081 gccgccggac gatttccaga atattgcgcc gtgtgacaaa gacatcgccg atgttgcgta
     1141 ctgtttccgg gcgaaacccg gatcacatta cgatacggtc tgtgtcccga aggagactgt
     1201 gcgcgaggaa ccgtcgtgac ccaccaaccg cggaatgaac ctccggcccg agccggcacc
     1261 attctcgtcg tcgatgacga tgcggagatc cgcagcctcg ttgccgagta tctcgggcaa
     1321 cagggttacg aggtggtggt cgcccgcgac gggaccgcga tgttcacggc gctcgacacg
     1381 cgccgtccgg atctcgtcgt actcgacgtg atgcttcccg gcgaggacgg cctcgcgctg
     1441 tgccgccggc tgcgtgcgca gtcggacctg ccggtgatca tgctgaccgc ccgcggcgac
     1501 gacatcgatc gcatcatcgg catcgaaatg ggtgctgatg attacctcgc gaaaccgttc
     1561 aatccgcgcg agctgctggc gcgcattcgc agcgtgcttc gacgcgtgcg tgcgccgccg
     1621 gctgccgccg ggcaggtgcg gcaactgcgt ttcgcgggat ggacgctcga ctgcgtcgcc
     1681 cgccacctcg tcgatgcgga cggagtcgtc gtgccgatga gcggcgcgca gttccggctt
     1741 ctcatggctt tcgtcgatcg cccccagcgg gtgctcgacc gcgaccagct cctcgacgcc
     1801 acgcgcggca tcaccgcgga tgcgttcgat cgcagcatcg acgtccagat aagccggctg
     1861 cgacagatgt tgcgcgacaa cgggcgcagg ccgcggatca tcaagacggt gcgcaacggc
     1921 ggctacgtgc tcgcggcgac cgtcgagcgg atcggctgat gcggctcgtt ccgcgctcgc
     1981 tgttcgggcg cacgttgctg gtgctcgtgg ccggtctcgt gctcgcccag gttgcgagcc
     2041 agacgctcaa tttcttcgat cgcggaagcg gcgtgtatcg gctcgccgca cagcagacgg
     2101 cgcttcgcat cgcacgcgcg gcgcgggtgc tcaaccgcgt gccgccgcac gagcggcagg
     2161 cgatcgtcga cgaaatgaac ggcggcagtt tcagcgtcgc acttgccaat gcgccggtcg
     2221 gcatggcggt cggattcgcc gggtacgacc gttacgagcg cgagctgtcg gcgctgatcc
     2281 gccattacct gcgcgccgac tggcgcacgt cggtcgtgat caccagcctc ggccgcgggg
     2341 aacgcccgcg gaactacgag gcggtcgata cgacaccg
//
LOCUS       DASUCJ010000644         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1199117, whole genome shotgun sequence.
ACCESSION   DASUCJ010000644 DASUCJ010000000
VERSION     DASUCJ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199117"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFU90_07685"
     CDS             <1..800
                     /locus_tag="VFU90_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5479700.1"
                     /translation="YYEESGVIRDMFQNHLMQLLTLTAMEPPNAFDADAVRGEKAKVL
                     GAIHPIAPEDADDMSVIGQYDAGEVNGEHVIAYRDEPGVAKDSRVPTYAAIRFTVDNW
                     RWHGVPFFLRSGKRLAKHATEIAVQFKEPPHSLFRIRGGLPIERNVLVMRIQPDEGIS
                     LRFETKVPGVDVQMAPVRMVFNYADAYGQSTHPAYETLLLDCMLGDATLFARWDEVEA
                     SWRIIDPIIDGWKNPGLVKLPNYTSGTWGPSAADALMAKDGAAWREP"
     gene            785..>1340
                     /gene="rpiA"
                     /locus_tag="VFU90_07690"
     CDS             785..>1340
                     /gene="rpiA"
                     /locus_tag="VFU90_07690"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU5479701.1"
                     /translation="MAGAVTDSRAAAKRDAAVRAVEAVRSGAVVGLGTGSTAAFAIEE
                     LGRRVAAGTLRDIVGIPTSNASHHLALEYGVPLTTLERHPRVDVTIDGADEIDPAGRV
                     LKGGGGALLREKIVATRTARWILVVDPGKLVPHLGARYPLPVEVVTFGWQAHLDALRA
                     LGAEPMLRLGGAEHPFVTDEGHYLI"
BASE COUNT          219 a          448 c          465 g          208 t
ORIGIN      
        1 cgtattacga agagtcgggt gtgattcgcg acatgtttca gaaccacctg atgcagctgc
       61 tcacgctcac cgcgatggag ccgcctaacg cattcgacgc cgatgccgtt cgcggcgaga
      121 aggccaaagt gctcggcgcg attcacccga tcgcgcctga agatgccgac gacatgtcgg
      181 tgatcggaca gtacgacgcc ggagaggtga acggcgagca cgtgatcgcg taccgcgacg
      241 agcccggcgt cgccaaggac tcgcgggtgc cgacgtacgc cgccatccgg ttcacggtgg
      301 acaactggcg ctggcacggc gtgccgttct ttctgcggtc gggcaagcgc ctcgccaagc
      361 acgccaccga gatcgccgta cagttcaagg agccgccgca ctcgctcttc cgcatccgcg
      421 gcggcctgcc gatcgaacgc aacgtgctcg tgatgcgcat ccagccggac gaaggcatct
      481 cgttgcgatt cgagaccaag gtgcccggcg tggatgtgca gatggcgccc gtccgcatgg
      541 tgttcaacta cgccgacgca tacgggcagt cgacgcaccc ggcgtacgag acgctgctgc
      601 tcgactgcat gttgggcgac gccacgttgt tcgcccgctg ggacgaggtg gaagcgtcgt
      661 ggcggatcat cgatccgatc atcgatggat ggaagaatcc cggtctggtc aaattgccga
      721 actacacttc cggcacctgg ggaccatcgg cggccgacgc gctgatggcg aaggacggcg
      781 ccgcgtggcg ggagccgtga cggattcgcg cgcggccgcc aagcgcgacg cggccgtgcg
      841 cgcggtcgag gccgtgcgct ccggcgcggt cgttgggtta ggcaccggca gcacggcggc
      901 gttcgcgatc gaagagctcg gccgccgcgt cgccgccggg acgctgcgcg acatcgtcgg
      961 cattccgacg tcgaacgcgt cgcatcatct ggcgctcgaa tacggcgtcc cgctcaccac
     1021 cctcgagcgc cacccgcgcg tggacgtcac catcgacggc gcggacgaga tcgatcccgc
     1081 cggtcgcgtg ctcaagggcg gcggcggcgc gctgctgcgc gagaagatcg tcgccacgcg
     1141 gacggcgcgg tggatcctcg tcgtcgaccc gggcaagctc gtgccgcacc tcggcgcgcg
     1201 ctacccgctg ccggtggaag tggtcacgtt cggctggcag gcccatttgg acgcgctgcg
     1261 tgcgttaggc gcggagccga tgctccggtt aggcggcgcc gaacacccgt tcgtgaccga
     1321 cgaaggccac tacctgatcg
//
LOCUS       DASUCJ010000645         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_7056, whole genome shotgun sequence.
ACCESSION   DASUCJ010000645 DASUCJ010000000
VERSION     DASUCJ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7056"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /gene="infB"
                     /locus_tag="VFU90_07695"
     CDS             <1..2257
                     /gene="infB"
                     /locus_tag="VFU90_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5479702.1"
                     /translation="HEPIPQLRPVPAGQSSIVRRSPPPPTTTASASPGPQVGLPGRPG
                     VAPRPGQVMPGQGGKAPGVPARPAAMQQTIAAPVRRHAGNGPFRPMGPGQRAPSGPGR
                     PGMPTGVADMPSPSTGSGRPGDRIRTHEDKASAKKDREKELLLEKERQRKKKGDHVAP
                     PVQRALETIEIPDLLTVQELATSMIIPVKDVITELIKMGTMATINQNIPSDVAIAVGR
                     KFGFNAVVKEAGEEVTVEQEEDKPEMMTPRPPVVTVLGHVDHGKTSLLDRIRQANVAA
                     GEAGGITQKIGAYTVEQHDRKITFIDTPGHEAFTAMRARGAKVTDVAILVVAADDGVM
                     PQTKEAIAHVKAAGVPIVVAVNKMDKPDAQPDRVKQQLSEEGLQPVDWGGTVEMVPVS
                     AKTGDGIGQLLDTVLLEADIRDLKANRNRRASGVVIESALDRGRGAVATVLVQNGTLR
                     VGDIIVVGGSFGKVRALIDDKGKQVKKAGPSIPVEIMGLSDVPSAGDTLAAVSDERVA
                     REAAEKRATRRRDVRMAATGSQRVSLETFMTMPAEGKKTLNLILKADGQGSLEALRAR
                     MESLSNDDVDIRVIHGGVGAISPNDVNLASASNAVLIGFNIRPDETARRLAENEGVDL
                     RFYQVIYEIEEDLKKAMRGMLAPVVREVTLGRAEVRQVFKVSKVGTIVGCYVTSGKIA
                     RNSKARVLRDSAVVFDGEIESLRRFKDDVREVAEGFECGIQIAKFQDLKEGDVIEAYA
                     METVAPELVRA"
     gene            2254..2607
                     /gene="rbfA"
                     /locus_tag="VFU90_07700"
     CDS             2254..2607
                     /gene="rbfA"
                     /locus_tag="VFU90_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5479703.1"
                     /translation="MKPQRLAKIDHEIQRVLGTLISQELQDPRLSFVTVTRVEVSDDL
                     RHCKVFVSVIGDRQAARQSLDALRHAGRFLRGELGRKIDLRHTPELVFVEDRSAERAI
                     ALAKTLREAAKEKPA"
     gene            2604..3584
                     /locus_tag="VFU90_07705"
     CDS             2604..3584
                     /locus_tag="VFU90_07705"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU5479704.1"
                     /translation="MIERDEEIATTAEVVDELRARQAFVMVSHVKPDGDTLGAGLALG
                     LALKRLGKRVCYFQQDPVPRNLRFLPEAEFVSRELPADLPPDTLYVFCDMSDHARAGE
                     YLPAVTRENMLDVDHHLGNSLFGKLNFVLETECSTGTVVMHLLRGLGVPIDKDIATCI
                     LTTIMTDTGGFMHSNTSPEALELAAELMRAGAEKEEITEQIFLRRRVAATKLLAKIVD
                     AMVFVHDGRYCYSYVDDAMLAATGADGEDTEDVVNVLRGQDGVEAAALFKAIDGEIRV
                     SLRSSGRINVQAAAKRLGGGGHFRASGLTFRGTLAEAFGAVDAALVAEGL"
     gene            3581..>3782
                     /locus_tag="VFU90_07710"
     CDS             3581..>3782
                     /locus_tag="VFU90_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479705.1"
                     /translation="MSHGAVPLAAGIFLTVFGAAGVWFLSRHFPGARFLRASYGLIAG
                     GGGLFVAWALSNVLAVGVAAAAL"
BASE COUNT          688 a         1220 c         1309 g          565 t
ORIGIN      
        1 ccacgagccg attccgcagc tccgcccggt tccggccgga caatcctcga tcgtgcgccg
       61 ttcgccgccg ccgccgacca cgaccgcctc ggcttcgccg ggcccgcaag tcggcttgcc
      121 gggccgcccc ggggtcgcgc cgcggcccgg acaagtcatg cccggccagg gcggcaaggc
      181 acccggcgtg ccggcccgtc cggccgcgat gcagcagacg attgccgcgc cggtccgccg
      241 gcatgccggc aacggtccgt tccgcccgat gggcccgggc cagcgcgctc ccagcggtcc
      301 cggccgtccg ggaatgccga ccggcgtggc cgacatgccg tcgccgtcga ccggaagcgg
      361 gcgccccggc gaccgcatcc gcacgcacga agacaaagcc tcggcaaaaa aggatcgcga
      421 gaaagaactg ttgctcgaaa aggagcggca gcgcaaaaag aagggcgacc acgtcgcacc
      481 gccggtgcag cgcgcgctgg agacgatcga gattcccgac ctgctcaccg tgcaagagct
      541 cgccacgtcg atgatcattc cggttaaaga cgtcatcacc gagctcatca agatgggcac
      601 gatggcgacg atcaatcaga acattcccag cgacgtggcg atcgcggtcg gacggaagtt
      661 cggcttcaac gccgtcgtga aggaagccgg cgaagaagtc accgtcgagc aagaggaaga
      721 caagcccgag atgatgacgc cgcgtccgcc ggtcgtcacg gtgctcggtc acgtcgatca
      781 cggcaagacc tcgctgctcg accggatccg ccaagccaac gtcgcggccg gcgaagccgg
      841 cggaatcacc cagaagatcg gcgcgtacac ggtggaacag cacgatcgca agatcacgtt
      901 catcgatacg cccggtcacg aagcgttcac cgcgatgcgc gcgcgcggcg cgaaggttac
      961 cgacgtggcg atcctggtgg tcgccgccga cgacggcgtc atgccgcaaa ccaaagaagc
     1021 gatcgcccac gtcaaagcgg cgggcgttcc gatcgtggtc gcggtcaaca aaatggacaa
     1081 gccggacgca cagcccgacc gcgtgaagca gcagctctcc gaagagggac tgcagccggt
     1141 cgattggggc ggcaccgtcg aaatggtgcc ggtctcggcg aaaaccggcg acggcatcgg
     1201 ccaattgctc gacacggtct tgctggaggc cgacattcgg gatctcaaag ccaaccggaa
     1261 ccggcgcgcg agcggcgtgg tgatcgaatc ggcgctcgac cgcgggcgcg gtgcggtcgc
     1321 gaccgtcctg gtgcaaaacg ggacgctgcg cgtcggcgac atcatcgtgg tcggcggatc
     1381 gttcggcaaa gtgcgcgcgc tgatcgacga caagggcaaa caagtcaaga aagccggacc
     1441 gtcgattccg gtcgagatca tgggcctgtc ggacgttccg tcggccggcg acacgctcgc
     1501 ggcggtcagc gacgagcgcg tcgcgcgcga agccgccgag aagcgtgcta cgcgccgtcg
     1561 cgacgtccgg atggcggcca ccggctcgca gcgcgtctcg ctggagacgt tcatgacgat
     1621 gccggccgag ggcaagaaga cgctcaacct catcctcaaa gccgacgggc aaggctcgct
     1681 ggaagcgctg cgcgcgcgga tggagtcgct gtcgaacgac gacgtcgaca ttcgcgtgat
     1741 tcacggcggg gtcggcgcga tctcgcccaa cgacgtcaac ctcgcgagcg cctcgaacgc
     1801 cgtgctgatc gggttcaaca ttcgtcccga cgagacggcg cggcggctgg ccgagaacga
     1861 aggcgtcgac ttgcgcttct atcaggtgat ctacgagatc gaggaagacc tcaagaaggc
     1921 gatgcgcggc atgctcgcgc cggtcgttcg cgaggtcacg ctcgggcgtg ccgaagtgcg
     1981 gcaggtgttc aaggtcagca aggtcggtac gatcgtcggc tgctacgtca ccagcggcaa
     2041 gatcgcccga aactccaagg cgcgcgtgct gcgcgactcg gcggtggtct tcgacggcga
     2101 gatcgagtcg ctgcgccgct tcaaagacga cgtgcgcgaa gtcgccgaag gcttcgagtg
     2161 cggcattcaa atcgccaaat tccaggacct gaaagaaggc gacgtgatcg aagcgtacgc
     2221 gatggagacg gtcgctcccg agctggtgcg cgcgtgaagc cgcagcgtct ggccaagatc
     2281 gatcacgaga tccagcgcgt tctcgggacg ctgatctcgc aggagctgca agatccgcgg
     2341 ctgtcgttcg tcacggtgac gcgggtcgaa gtctccgacg acttgcgcca ctgcaaggtg
     2401 ttcgtatcgg tcatcggcga tcgccaggcg gcgcgtcagt cgctcgacgc gctccgtcac
     2461 gccgggcggt tcttgcgcgg cgagctgggg cgcaagatcg atttgcgcca cacgccggag
     2521 ctggtgttcg tcgaggaccg ctcggccgag cgggcgatcg cgctggcgaa aacgctgcgc
     2581 gaagccgcca aggagaaacc ggcgtgatcg agcgcgacga ggagatcgcg accaccgccg
     2641 aggtcgtgga cgagctgcgc gcgcggcagg cgttcgtgat ggtgagccac gtcaaacccg
     2701 acggcgacac tctgggcgcg ggcttggcgc tcggcctcgc gctcaaacgg ctcggcaaac
     2761 gcgtctgcta tttccaacag gatccggtgc cgcgcaattt gcggtttctt ccggaagcgg
     2821 aattcgtctc gcgcgagctg cccgcggatc tgccgcccga cacgctgtac gtgttttgcg
     2881 acatgagcga ccacgcgcgc gccggcgagt atctcccggc ggtgacgcgc gagaacatgc
     2941 tcgacgtcga tcatcacctc ggcaactcgc tgttcggcaa gctgaacttc gtgctggaaa
     3001 ccgagtgctc gaccggcacc gtcgtgatgc acctgttgcg cgggctgggc gtgccgatcg
     3061 acaaggacat cgcgacctgc atcctgacga cgatcatgac cgacaccggc ggtttcatgc
     3121 actcgaatac gtcgcccgag gcgctggagc tggccgccga gctgatgcgc gcgggcgccg
     3181 aaaaggaaga gatcaccgaa cagatattcc tgcgccggcg cgtggccgca acgaaactgc
     3241 tggcgaaaat cgtcgacgcg atggtcttcg tgcacgacgg gcgttactgc tactcgtacg
     3301 tcgacgacgc gatgctggcc gcgaccggcg cggacggcga agataccgaa gacgtggtca
     3361 acgtcttgcg agggcaggac ggcgtcgaag cggcggcgct attcaaagcc atcgacggcg
     3421 aaattcgcgt gagcctgcgt tccagcgggc gcatcaacgt acaggccgcc gccaagcggc
     3481 tcggcggcgg cggccatttt cgcgcgtccg gcttgacctt tcgcggcacg ctggcggagg
     3541 catttggcgc ggtggatgcc gcgctcgtcg ccgaaggcct atgagccacg gcgccgttcc
     3601 gctggccgcc ggaatctttc tcaccgtctt cggcgcggcc ggagtctggt tcttgagccg
     3661 gcattttccg ggcgcgcgat ttttgcgcgc gagctacgga ttgatcgcgg gcggcggggg
     3721 gctgttcgtg gcgtgggcgc tttcgaacgt gctcgcggtg ggcgtggcgg cggcggcgct
     3781 ag
//
LOCUS       DASUCJ010000646         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1290686, whole genome shotgun sequence.
ACCESSION   DASUCJ010000646 DASUCJ010000000
VERSION     DASUCJ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290686"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VFU90_07715"
     CDS             complement(<1..1057)
                     /locus_tag="VFU90_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEU5479706.1"
                     /translation="MIIGLVLAIVLFWQATPSTSPARDQIDYGQFLTLVQQDHVSSVT
                     IDSATGKITGVYAPKGFSPNDNNRTQFSTQGPVNNQGIPDADLALLDKHNVARKYKGH
                     STDWLAALVPWVILFVFIGALWWFIARRAQGQMGAVMNIGRSRAKVYNTDKPKTTFGD
                     VAGYGAVKEEITEVVDFLKMPAKFKDIGARIPKGVLLVGPPGTGKTLIARAVAGEAGV
                     PFISITGSDFMEMFVGVGAARVRDLFQTARKQAPAIIFVDEIDSIGRKRGAGLGGGHD
                     EREQTLNQMLSEMDGFEATEGIVMMAATNRPDILDPALLRPGRFDRQILVPLPTQDER
                     VEILKVHMRDKKMSSDVD"
BASE COUNT          219 a          448 c          471 g          269 t
ORIGIN      
        1 cgtcgacgtc ggacgacatc ttcttgtcgc gcatgtggac cttgaggatc tcgactcgct
       61 cgtcctgcgt gggcagcggc acgaggatct ggcggtcgaa ccggccggga cgcaacagtg
      121 cggggtcgag gatgtcggga cggttcgtcg ccgccatcat cacgatgccc tcggtcgcct
      181 cgaagccgtc catctccgag agcatctggt tgagcgtctg ctcgcgttcg tcgtgaccgc
      241 caccgagacc ggcgccgcgc ttgcggccga tggagtcgat ctcgtcgacg aagatgatcg
      301 cgggcgcttg cttgcgcgcc gtctggaaca agtcgcgcac gcgcgcggcg ccgacgccga
      361 cgaacatctc catgaagtcg gagccggtga tcgaaatgaa cggcacgccg gcctcgcccg
      421 cgactgcacg cgcgatcagc gtcttgccgg tgccgggcgg accgacgagc aacacgccct
      481 tgggaatgcg tgcgccgatg tctttgaact tcgccggcat cttcaagaag tcgacgactt
      541 cggtgatttc ttctttcacc gcgccgtaac cggcgacgtc gccgaacgtc gtcttcggtt
      601 tgtcggtgtt gtagaccttg gcgcgcgatc tcccgatgtt catgaccgcg cccatctgcc
      661 cttgggcgcg ccgcgcgatg aaccaccaca gcgcgccgat gaacacgaac aagatcaccc
      721 atggaacgag ggccgcgagc cagtcggtgc tgtggccctt gtacttgcgc gcgacgttgt
      781 gcttgtcgag cagtgcgaga tcagcgtcgg ggatgccttg gttgttgacg ggaccctgcg
      841 tcgagaactg tgtgcggttg ttgtcgttcg gcgaaaagcc cttgggcgcg tagacgccgg
      901 tgatcttccc ggtcgcggaa tcgatcgtca cgctcgagac gtgatcctgt tgcaccaacg
      961 tgaggaactg gccgtagtcg atctggtcgc gcgcgggaga cgtgctcggc gttgcttgcc
     1021 agaacaatac gatcgcgagg acgaggccga tgatcaccca gccgatccaa cgcggccgcg
     1081 acgacgacga acgcggcggc gaacccgggc gcggcggacg gccgggcggt tccggcgggc
     1141 gccactgatc ttccgggcca ggactcatct gttgttctcc acgcgatcgc ttcggaacat
     1201 gctacggcgg gcagctgtgc attcccgttc cgggtacacg ctcgaccgcg aaagcggtct
     1261 gagtagcttt atccggtgga tccccagccc tgggacgtgc cgcccgccga tctcgcgccg
     1321 cccctccgct ggggcatcgg tgacttcttc tgggtgtacg tcgtcggttt gttggtgagc
     1381 caggtcgcgg cggcgatcgc ctacggc
//
LOCUS       DASUCJ010000647         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1000408, whole genome shotgun sequence.
ACCESSION   DASUCJ010000647 DASUCJ010000000
VERSION     DASUCJ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000408"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFU90_07720"
     CDS             <1..1091
                     /locus_tag="VFU90_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479707.1"
                     /translation="TPKLLNTLQTTLNNANDLTVSTKRSLSQLSGELLALGSSLQGNL
                     SAAGANVVALTDTLNGTATKDSAKVDQLLDQLNATSVSLNKSMGALQGLATDPQLKSN
                     VLTTTQSIADATQTLAGLLKDLRTVSSDPQTQAQIRNTIANLDATMQKANSLLGELGG
                     TSSVYGVDANATPAPPVVPGTPYPLPSGGAAPPSEPSQATQSSQSRTRLQGKLANVAR
                     NLVALQLRLSGLSVQKNAGLNPVLTSSQGPLGDLNLVFLPHASTSLMVGANAIGNNTT
                     WNAVLMKGSDTGFRFGGGVLYSQIGILAQMNALKGFGLETRVYDLTYPMIDLYGNIRI
                     MKGAELFFGERDLNHASRRNTYGLQYTF"
     gene            1110..>1556
                     /locus_tag="VFU90_07725"
     CDS             1110..>1556
                     /locus_tag="VFU90_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5479708.1"
                     /translation="MDQTRPRVGIIGAGAMGTLFGFHLAELCDVTILDRNPAVNDAIA
                     RDGLSVNDLPARPVSIARHPRELYGSQVLFLFVKAVDTLRALRPFAGELNPSSPIVSL
                     QNGVGNEDAIKTALGGAVPVILGITTESSHSVGAGRVRSSGEGHSII"
BASE COUNT          291 a          514 c          488 g          263 t
ORIGIN      
        1 ggacgccgaa actgctcaat accctgcaaa cgacgctcaa caacgccaac gatctcacgg
       61 tcagcaccaa acgatcgctg tcgcagctat cgggcgaatt gctcgccttg ggctcgagcc
      121 tgcaagggaa tttgagcgcc gccggcgcca acgtcgtcgc gctgaccgac acgctcaacg
      181 gtacggcgac caaggactcg gccaaggtag accagctgct cgatcaactc aatgccacct
      241 cggtgtcgct caataagtcg atgggagcgc tccaggggct cgcgaccgat ccgcagctca
      301 agtcgaacgt tctgacgacc acgcaaagta tcgccgacgc gacccaaacg ctggccggcc
      361 tgttgaaaga cctccgcacc gtcagcagcg atccgcagac gcaggcgcag attcgcaaca
      421 cgatcgccaa tctcgacgcc acgatgcaga aggccaactc gctgctcggc gagctgggcg
      481 gcaccagcag cgtttacggg gtggacgcca acgcaacgcc ggcgcctccg gtcgtgccgg
      541 gcacgccata cccgctgccc tcgggtggcg cggcgccgcc ctcggagcct agccaggcga
      601 cgcaatcctc gcagtcgcgc acgcgtctgc aaggtaaact cgccaacgtc gcgcggaatc
      661 tggtcgcgct gcagctgcgg ctgagcggcc tctcggtgca gaagaacgcg ggcttgaatc
      721 cggtgcttac cagcagtcag gggccgctcg gcgacctcaa cctcgtcttc ctgcctcacg
      781 cctcgacgag cctgatggtg ggggcgaatg cgatcggcaa caatacgacc tggaacgccg
      841 tgctaatgaa gggctccgac accggcttcc gcttcggcgg cggcgtgttg tattcgcaga
      901 tcggcatctt ggcgcagatg aacgcgctca aggggttcgg cttggagacg cgcgtttacg
      961 acttgaccta tccgatgatc gatttgtacg gaaatatccg gatcatgaag ggcgcggagc
     1021 tgttcttcgg cgagcgcgac ctgaatcacg ccagccgtcg taatacctac ggcctccagt
     1081 acacctttta gcgcggagcg ccgtcggcga tggatcagac tcgaccgaga gtaggaatca
     1141 tcggtgccgg cgcgatgggc acgctgttcg gctttcattt ggccgagctg tgcgacgtca
     1201 cgatcctcga ccgtaacccg gccgtcaacg acgcgatcgc ccgcgacggt ctatcggtca
     1261 acgatctgcc ggcccgtccg gtgtcgatcg cgcgccaccc gcgcgagctg tacgggtcgc
     1321 aggtgttgtt cctgttcgtg aaagcggtcg acaccctgcg cgcgctgcgt ccgttcgcgg
     1381 gcgagctgaa cccgtcgtcg ccgatcgtgt cgctgcagaa cggcgttgga aacgaagacg
     1441 cgatcaaaac tgcgctgggc ggcgcggtgc cggtgattct cggtatcacc accgagtcgt
     1501 cgcactccgt cggtgccggg cgcgtccgca gctcgggcga ggggcactcg atcatc
//
LOCUS       DASUCJ010000648         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_144828, whole genome shotgun sequence.
ACCESSION   DASUCJ010000648 DASUCJ010000000
VERSION     DASUCJ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144828"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFU90_07730"
     CDS             <1..543
                     /locus_tag="VFU90_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479709.1"
                     /translation="TFASCLAVDSEDYACTNDLGSLYLDRKRYADARRELLRAHQLAP
                     EGPEALVNLGYLSDELGNRPAAVAYYAEAIYVWPYFADAYVNLSFDQIRQGSIDQAEG
                     VLRKGLSIAPKDPRIHYLLGVVDEVHGKRAEAVAEFRFAESSPDPEVANSARENVSDI
                     ERTGASAGARPVPSPPSPHR"
     gene            complement(651..>1077)
                     /locus_tag="VFU90_07735"
     CDS             complement(651..>1077)
                     /locus_tag="VFU90_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479710.1"
                     /translation="AIAAIATSKYDLIVMNYANADMVGHTGKWGPTVEALELLDACLD
                     RLARAVCEAGGLLAITADHGNAEEKLDAAGNPLTAHTTNPVPLLLIAPALHGTLAPGG
                     KLGDVAPTLLRLMHLPVPPAMTGDNLFTPSAESSRPLSG"
BASE COUNT          192 a          338 c          362 g          185 t
ORIGIN      
        1 accttcgcga gctgtctcgc cgtcgattcc gaagactacg cgtgtaccaa cgatctcgga
       61 tcgctctatc tcgatcgcaa acgctatgcc gacgctcggc gcgagctgtt gcgggcgcat
      121 caactcgccc ccgagggacc cgaggcgctg gtgaacctcg gctacctctc cgacgaactg
      181 ggaaatcgcc cggccgcggt ggcgtattac gccgaggcga tctacgtgtg gccgtatttc
      241 gcggacgcgt acgtgaatct aagcttcgat caaatccggc aaggctcgat cgatcaggcg
      301 gaaggcgtcc tgcgcaaagg gctgtcgatc gcgccgaagg acccgcggat ccactatctg
      361 ctgggcgtcg tcgacgaagt gcacgggaag cgggcggaag cggtcgccga atttcgattc
      421 gccgaatcgt cgccggaccc ggaagtcgcc aactcggcgc gcgagaacgt cagcgacatc
      481 gagcgaacgg gggctagtgc aggtgcgcgg ccagtgccat cgccaccgtc gccgcaccga
      541 tgagcacgaa ccagaccatc acgcgtgcca gaaccaattc ttggttgacg tcggggtgct
      601 tgcgcatcgg gggtcgcctc ccttcggtga actcccgcta tcgaaccacg tcaacctgaa
      661 agcggtctgg acgattccgc cgagggcgta aaaaggttat cgccggtcat cgccggcggt
      721 accggcagat gcatgagccg caagagcgtc ggcgcgacgt cgccgagctt cccgccgggc
      781 gcgagcgtgc cgtgcagggc gggagcgatc aagagcagcg gaaccggatt ggtggtatgc
      841 gcggtaagcg ggtttccggc cgcgtcgagt ttttcttcgg cgttgccgtg gtcagcggtg
      901 atcgcgagca ggccgccggc ctcgcagacg gcacgggcga ggcgatccag gcaggcgtcg
      961 agcagctcga gagcctcgac ggtgggcccc cacttcccgg tgtgtccgac catgtcggcg
     1021 ttcgcatagt tcatgacgat cagatcgtac ttgctcgtcg cgatcgcggc gattgcc
//
LOCUS       DASUCJ010000649         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_236020, whole genome shotgun sequence.
ACCESSION   DASUCJ010000649 DASUCJ010000000
VERSION     DASUCJ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236020"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFU90_07740"
     CDS             <1..894
                     /locus_tag="VFU90_07740"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479711.1"
                     /translation="ARGLLEARPLPFVKGADIGFVRVANPIAENEAYLRRDLLDTLAR
                     RAEYNLARMQRDVRLFEIGSVFMPSSEALPVEELRAALIVMGHRRPPHWTETRPPDVD
                     EWDAKALAVELAHSAYPSAEIGLESDRDSDWLWTIAVDGTTRGGVRRVALDAPVWAAP
                     AFGVELTLLTVPSDFVAQPGASRYDSTAAASKPRHVQYRPLPDTPAAEFDLALLVPND
                     MPASRVEEVIRGAGGELLEQVAIFDEYRGDAVPNGYRSLAWRLTFRHPERTLRDKEVA
                     GRRDKLLRTLEGELGVRQRTT"
     gene            1162..1455
                     /locus_tag="VFU90_07745"
     CDS             1162..1455
                     /locus_tag="VFU90_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5479712.1"
                     /translation="MSVAHKSTVKVTILGEEYAIRSDETPEHTRAVAEHVDATIRALM
                     NAGVVVESQKAAILAAMQITSELFKSRQGPEELAERMRNISSEVRRMLPPQKR"
     gene            1715..>2239
                     /locus_tag="VFU90_07750"
     CDS             1715..>2239
                     /locus_tag="VFU90_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEU5479713.1"
                     /translation="MSVTALYAAFGVVLILAVAVFFVLGRAAGRAAEHRAQIAAKSTA
                     EETATRITGEARREAETLRKSAIVAGKEETIKLREAWEVDAGRRREELERAERRMQER
                     ETGLDRKYDILDQRDKELGRRGSDLGRREKIVGERESELDKLIAEERRRLEQLAGMSA
                     RDAKAELMSRLAEEA"
BASE COUNT          420 a          709 c          758 g          352 t
ORIGIN      
        1 gcgcgcggcc tgctcgaggc gcgcccgctc ccgttcgtca aaggcgcgga catcggcttc
       61 gtccgcgtgg ccaatccgat tgccgaaaac gaagcgtatc tccggcgcga cttgctcgac
      121 acgctcgcgc gacgcgccga gtacaacctg gcgcgcatgc agcgagacgt gcgactgttc
      181 gagatcggat cggtgttcat gccgtcgtcc gaggcgctgc ccgtggaaga gctccgcgcc
      241 gcactcattg tcatgggaca tcgccggccg ccgcactgga ccgagacgcg gccacccgat
      301 gtcgatgagt gggacgccaa agcactcgcc gtcgaattgg cgcactcggc ctatccgtcg
      361 gcagagatcg gcctcgagtc cgatcgcgat tcggactggc tctggaccat cgccgtcgat
      421 ggaacgacac gaggcggcgt tcgtcgggtg gcgctcgacg cacccgtgtg ggccgcgccc
      481 gcgttcggcg tcgagctgac gctgctcacc gttccgtccg acttcgtcgc gcagcccgga
      541 gcgtctcgat acgactcgac cgcggccgcg tcgaaaccgc ggcacgtgca gtaccggccg
      601 ctgccggaca cgccggcggc ggagttcgat ctggcgctcc tcgtgccgaa cgacatgccc
      661 gcctcgcgtg tggaggaggt gatccgtggc gcgggcggcg agctgctcga gcaggtggcg
      721 atctttgatg aataccgcgg cgacgccgtg cccaacggat atcgaagtct cgcgtggcga
      781 ttgacgttcc gccatccgga acgcactcta cgggacaagg aggtagcggg tcgtcgcgac
      841 aagctcctgc gcactctcga aggagaactc ggtgtccgac aacgaacgac ctgatctcgc
      901 cgcgtttcag gagctggaac agctcatcca tgcgctggca gaggaaatgg cggcgtggcg
      961 acgccgggcg cacgaagcgg aatcgcgttt gcgcacgctg tccaacggat cgggcagctc
     1021 gagcaagtcc ggcgcgcgat ccgacctgga acgggagaac gaagaccttc gtcggcgtct
     1081 cgacagcgca caacaacgca cgaaacaact cctggaccgt ctgcggttcc ttcggcagca
     1141 acacgaactc ggagcagatc gatgagcgtc gcccataagt cgaccgtgaa agtcacgatc
     1201 ctgggcgagg agtacgcgat tcgcagcgac gaaacgccgg agcacactcg cgcggtggcc
     1261 gagcacgtcg acgcgacgat ccgcgcgctg atgaacgccg gggtcgtcgt cgaatcgcag
     1321 aaagcggcca tccttgcggc catgcaaatc acgtccgagc tgttcaagtc gcgacagggc
     1381 ccggaggagc tcgccgagcg gatgcgaaac atctcgtccg aagttcgtcg catgctgccg
     1441 ccgcaaaaac ggtaaggcgg acaagacaga caaaggccgg acacggccct gtccgttttg
     1501 tgcgccttac ctcttgcttg aggacacctg cgacgcgtag tttgtgtgag tcctcaaggg
     1561 ttttccccta gtcgcatgtg ccggttgcag ttagcgttgt ccgcctcgca ccctacgtcg
     1621 gtgctgggcc ggcagcagct acgtgattgg ccgcggcgcc ctggggcgtt tccacgatcg
     1681 cgtgacgcgt gcgtcgcgtt ggagcataga tccaatgagc gtaaccgcgc tgtatgccgc
     1741 gtttggcgta gtactcatcc tcgccgtcgc ggtttttttt gtactcggac gtgcggccgg
     1801 acgcgcagct gaacatcgcg cgcaaatcgc ggccaagtcg acggcggaag aaacggcaac
     1861 gcgcatcacc ggagaagcgc gccgcgaggc cgagaccctc agaaaatcgg cgatcgtcgc
     1921 cggcaaagag gagacgatca agctccgcga ggcctgggaa gtcgacgccg ggcggcgccg
     1981 tgaggagctc gagcgtgccg agcgtcgcat gcaggagcgc gagacgggcc tcgatcgcaa
     2041 atacgacatt ctcgaccagc gagacaagga gctggggcgt cgcggcagcg atctgggccg
     2101 ccgcgagaaa atcgtcggcg agcgcgagag cgaactggac aagttgatcg ccgaggagcg
     2161 acgacgtctc gagcagttgg cggggatgtc cgcgcgcgat gcgaaagcgg agctcatgag
     2221 ccgcctcgcc gaggaagcg
//
LOCUS       DASUCJ010000650         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1305969, whole genome shotgun sequence.
ACCESSION   DASUCJ010000650 DASUCJ010000000
VERSION     DASUCJ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305969"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFU90_07755"
     CDS             complement(<1..225)
                     /locus_tag="VFU90_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479714.1"
                     /translation="MSISALFVRRPVMTMLVMIGILIFGIVGYRLLPVNALPNVDFPT
                     IQVQAQLPGASPDTMASAVATPLEKQFSTIA"
     gene            complement(222..>1422)
                     /locus_tag="VFU90_07760"
     CDS             complement(222..>1422)
                     /locus_tag="VFU90_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5479715.1"
                     /translation="SRSLHAARAQESFGMTRARRTLTLAALAILVIGIGGWLMHQRSG
                     SRAATPKVVAAVPVTVATVARHDVPVRLYAIGNIEPYTTVAVKALVDGQIVAVHFAEG
                     DEVHQGAVLFEIDRRPFEAQLAQAQANLVKDRAVLDHAAEQEKRYKDLLAQKFISPDA
                     YGQVKANAESAAAQVRADQAAIEGVKLQLNYCTIRAPITGYAGKIMIQRGNLVKANDT
                     NALVVINQVRPIYASFSIPEQNLDAVRRYLADGELAVTASVPSSGSAPVPNPASAPVT
                     GKLTFIDNTTDVSTGTIKLKAEFANTGKELWPGQFVNVVLTLTLEKNAIVMPSTAVQN
                     GPDGQYVFVVGADGHVALRTVKIERTENGDAVVASGVTLGERVVTAGQLRLAPGVAVT
                     IDSAKGA"
BASE COUNT          224 a          469 c          480 g          249 t
ORIGIN      
        1 ggcgatggtc gagaactgct tctcgagcgg cgtcgcgacc gccgaggcca tcgtgtcggg
       61 gctcgcaccc gggagttgcg cctgaacctg gatcgtcggg aagtcgacgt tgggaagcgc
      121 gttgaccggg agcagccggt atccgacgat gccgaagatg aggatgccga tcatgaccag
      181 catcgtcatg accggacggc gaacgaacag cgccgaaatg ctcacgcgcc cttcgcgctg
      241 tcgatcgtca ccgcaacgcc cggcgcgagg cgaagctgcc cggccgtgac tacgcgctcg
      301 ccgagggtca cgcccgaggc gacaaccgcg tcaccgttct cggtgcgctc gattttcacc
      361 gtgcgcagcg cgacgtgccc gtcagcgccg acgacgaaca cgtactgacc gtcgggcccg
      421 ttctgcacgg cagtcgacgg catgacgatc gcattcttct ccagcgtcag cgtcagtacg
      481 acgttgacga actgccccgg ccagagctct ttgccggtgt tcgcgaattc cgccttcagc
      541 ttgatcgtgc cggtggagac gtcggtcgtg ttgtcgatga aggtgagctt ccccgtgacc
      601 ggcgcactcg ccgggttcgg aacaggcgca ctgcccgagc tcggaaccga cgcggtgacc
      661 gcgagctcgc cgtcggcgag gtacctgcgc accgcatcga ggttctgttc gggaatcgag
      721 aacgacgcgt agatcggacg cacctggttg atcaccacca gcgcgttggt gtcgttggcc
      781 ttcaccaggt tgccacgctg gatcatgatc ttgccggcgt agccggtgat cggcgcgcgg
      841 atcgtgcagt agttgagctg gagcttgacg ccttcgatcg ccgcctggtc ggcgcgtacc
      901 tgcgccgcgg cgctctcggc atttgccttc acctggccgt aggcgtcggg cgagatgaac
      961 ttctgcgcaa gcaggtcctt gtagcgcttc tcctgctcgg cggcgtggtc gagcacagca
     1021 cgatccttga cgaggttcgc ctgcgcctgc gcaagctgcg cttcgaacgg gcggcggtcg
     1081 atctcgaaca gcacggcgcc ctgatgcacc tcgtcgccct cggcgaagtg cacggcgacg
     1141 atctggccgt cgacgagcgc cttgacggcg acagtcgtgt acggctcgat attgccgatc
     1201 gcgtagaggc gaaccgggac gtcgtgtctc gcgaccgtcg cgacggtcac cggcacggcg
     1261 gcgaccacct tcggggtggc agcgcgcgag ccggagcgct ggtgcatcag ccatccgccg
     1321 atgccgatca ccagtatcgc caacgcggcc agcgtcagcg tgcgccgcgc tcgtgtcatg
     1381 ccgaaactct cctgggcgcg ggccgcatgc agggatcggc tc
//
LOCUS       DASUCJ010000651         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_572760, whole genome shotgun sequence.
ACCESSION   DASUCJ010000651 DASUCJ010000000
VERSION     DASUCJ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572760"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFU90_07765"
     CDS             complement(<1..686)
                     /locus_tag="VFU90_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479716.1"
                     /translation="MAMTSETSGAIVRVAEDNTDPIQPFAHSSGFSTAQRMANALASS
                     TMVPDAYRGNIANCLIALELAARIRASVLMVMNNLDIVHGRPSWRATFLIATVNTCGR
                     FTPIRFRFEGTRGTDAWGCRAVATDKASGEECVGTLITIALAKAEGWYGRNGSKWQTI
                     PEQMLQYRAAAFWVRIYCPELANGMRTTDEESDSVIDVVPEPVTTSTPAALEQSLQGP
                     IAATMPAAAPA"
     gene            complement(697..1524)
                     /locus_tag="VFU90_07770"
     CDS             complement(697..1524)
                     /locus_tag="VFU90_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HEU5479717.1"
                     /translation="MNTALELAHGFHADVAEGTYHARVPGLVSKHAIDLVRRSPAHYK
                     AWLDGAESEDREAFRLGRATHCALLEPYRFSCEYVVEPEFGDCRKTENKNRRNEWREE
                     HKNAIWLPASDHAAILGMAKAIAAHPVAGKLFTGGHAELTLRWRDAETGLECKARDDY
                     FRDDLGAVIDLKTAENAGAEAFRRSVVNFGYHRQHAHYARGHQALGRPLKNFVFVVVE
                     KAPPYAVAVYSLDSAAEAKGIEQVAEAMRTLAECVRVGRYPGYPENIQTLELPPWAA"
     gene            complement(1521..1799)
                     /locus_tag="VFU90_07775"
     CDS             complement(1521..1799)
                     /locus_tag="VFU90_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479718.1"
                     /translation="MSAPTIAEIIGDARSLHRASVLLRVDAQAVAGYSIPAGDALAAD
                     TAVLAESLRLFIGKLEAFELGHVIESIDAGIPPTDPTPGLSTWGKEGA"
BASE COUNT          302 a          649 c          664 g          344 t
ORIGIN      
        1 gcaggcgccg ccgcgggcat cgtcgcggca atgggcccct gaagcgactg ctcgagcgcg
       61 gcgggtgtcg acgtcgtcac gggctccgga acgacgtcga tcaccgaatc cgactcttcg
      121 tccgtcgtcc gcatcccgtt cgccaactcg gggcagtaga ttcgcaccca aaatgccgcg
      181 gcacggtact gcagcatctg ctcggggatc gtctgccact tggaaccgtt gcggccgtac
      241 cagccttcgg ccttcgcgag agcgatcgta atgagcgtgc ctacgcactc ttcgccgctc
      301 gccttgtccg tcgcgacggc tcggcagccc catgcgtcgg ttccgcgcgt tccctcgaat
      361 cgaaagcgga tcggcgtgaa gcgcccgcac gtattcacgg tcgcgatcaa gaacgttgcg
      421 cgccagctcg ggcgaccgtg gacgatgtcc agattgttca tcaccatgag gacggatgcg
      481 cggatacgcg cagcgagctc caaagcaata aggcaattag cgatgttccc gcgatatgca
      541 tcagggacca ttgtgctgga cgcgagcgcg ttcgccatcc gctgggcggt ggaaaacccg
      601 ctcgaatgcg caaacggctg gatcgggtcg gtgttgtctt ctgcaacgcg cacgatggcg
      661 ccgctggtct cactggtcat tgccataatt ggagcctcac gccgcccacg gcggcaattc
      721 gagagtttgg atgttctccg gatatcccgg atatcggccg acgcgcacgc attcggcgag
      781 cgtccgcatc gcctcggcga cttgctcgat gcccttcgct tccgcggccg agtcgaggct
      841 gtagaccgcg acggcgtacg gcggcgcttt ctcgacgacg acgaacacga agttcttgag
      901 cggtcgcccg agcgcttggt gcccccgtgc atagtgcgcg tgctggcggt ggtatccgaa
      961 attcacgacc gatcgccgga acgcctctgc gcctgcgttc tccgcggtct tcaggtcgat
     1021 gacggccccg agatcatctc ggaagtagtc gtcgcgcgcc ttgcattcga ggccagtctc
     1081 cgcgtcgcgc catcgaagcg tgagctccgc gtggccgccg gtaaagagct ttccggcaac
     1141 ggggtgcgct gcgatggcct tcgccatgcc gaggattgcc gcgtggtcgc ttgcaggcag
     1201 ccagattgcg ttcttgtgct cttcgcgcca ctcgttgcgg cggttcttgt tctcggtctt
     1261 tcggcagtcg ccgaactcgg gctcgacgac gtactcgcac gagaaccggt acggctcgag
     1321 cagcgcgcag tgcgtcgcgc gcccgagccg gaaggcctcg cgatcctcgc tctccgcgcc
     1381 gtcgagccac gccttgtagt gggccgggga gcggcggacg agatcgatcg catgcttgct
     1441 cacgaggccg gggacgcgcg cgtggtaggt gccctccgcg acgtcggcgt ggaatccgtg
     1501 tgcgagctcg agtgcggtgt tcacgcgccc tccttccccc aagtcgaaag ccccggcgtt
     1561 ggatcggtgg gcggaatgcc cgcgtcgatc gactcgatga cgtgccccag ctcaaaggcc
     1621 tcgagcttgc cgatgaagag acgcagcgac tccgcgagga ccgcagtgtc cgccgcgagc
     1681 gcatcgccgg ccggaatcga gtagcccgcg acggcttgcg cgtcgacgcg gaggagcacg
     1741 gaggcccggt gtaggctccg cgcgtcgccg atgatttcgg cgatggtcgg agcgctcacg
     1801 ggccgactcc cggccgcacg gtagcgacgg cggtctgcgc gacggcgcgc caggtcgccg
     1861 aatccgggtc gacgcgccga gccggcggca tcaggggcgc tgcggcaggc cccgggcgca
     1921 tgcacgaacg gtggaccggc tcgatggtcg cgacgacga
//
LOCUS       DASUCJ010000652         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_602797, whole genome shotgun sequence.
ACCESSION   DASUCJ010000652 DASUCJ010000000
VERSION     DASUCJ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602797"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1210)
                     /locus_tag="VFU90_07780"
     CDS             complement(155..1210)
                     /locus_tag="VFU90_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5479719.1"
                     /translation="MALMLALAGSTLAATSALARPVTSRGPLAPDEQRTIEIFEKSKR
                     SVVYISTSERVRDLWSRNVRTIPRGTGSGIIWDEAGHVITNYHVIAGASAATVRLNDG
                     RDYPATLVGASQFHDIAVLHIGVTSNPPPPLPLGASHDLKVGQRVYAIGNPFGLDWTL
                     TTGIVSALDRSIDDDNGNTIEHLIQTDAAINPGNSGGPLIDSAGRLIGMNTAIYSPSG
                     ASAGISFAVPADTINRVVTALIAHGRYARPTLGIEIDPDLNDLLSSRLGVEGVFVLRV
                     DPHGPAATAGLREAKIERGSIVPGDVIVSVGGKPVTTPSRLQARLDDFNPGDHVRLGV
                     LRNGKEIAVTVTLAAIR"
BASE COUNT          204 a          439 c          477 g          216 t
ORIGIN      
        1 ccgggcaggg gcgcgtcgct cgcgcaacgt cccggtgccg cccgatgttg cggcgcggac
       61 acgctcgggt caagcagagt aatcacccag cgaaaccgcc cggtctggcg ggtgaggcgg
      121 gccttcggcg tccgtcaacg gcttctggcg ccgctcagcg gatcgcggcg agcgtcacag
      181 tcaccgcgat ctccttgccg ttgcgcagca cgccgaggcg cacatggtcg ccgggattga
      241 aatcgtcgag gcgtgcctgt agccgggacg gggtcgtcac cggcttgccg ccgaccgaca
      301 cgataacgtc tccgggaacg atcgagcccc gctcgatctt cgcttcgcgc aagcccgccg
      361 tggccgcagg tccgtgcggg tcgaccctca gcacaaatac gccctccacg ccgaggcgcg
      421 agctcaggag atcgttgaga tcggggtcga tctcgattcc gagcgtcgga cgcgcgtagc
      481 ggccgtgcgc gatcagcgcc gtcacgacgc ggttgatcgt gtcggcgggc accgcaaagc
      541 tgattcccgc cgaggcgccg ctcgggctgt agatcgcggt gttcatgccg atcagtcgcc
      601 ccgcgctgtc gatgagcggg ccgccggagt ttccggggtt gatcgccgcg tcggtctgga
      661 tcaggtgctc gatcgtattg ccgttgtcgt cgtcgatcga gcggtcgagc gccgacacga
      721 tgccggtggt gagcgtccag tcgagcccga acggattgcc gatcgcgtat acgcgctggc
      781 cgaccttgag gtcgtggctc gcgccgagcg gcagcggcgg cggcggattc gaggtcacgc
      841 cgatgtgcag caccgcgatg tcgtggaact ggctcgcgcc gaccagcgtc gccgggtagt
      901 cgcggccgtc gttcaggcgc accgtggcgg ccgacgcgcc ggcgatcacg tgatagttgg
      961 tgatgacgtg gcccgcttcg tcccatatga tgcccgagcc cgtgccgcgc ggaatcgtgc
     1021 ggacgttgcg tgaccacagg tcgcgcacgc gctccgaggt cgagatgtag acgaccgagc
     1081 gcttcgactt ctcgaagatc tcgatcgtgc gctgctcgtc gggggcgagc gggccgcgag
     1141 atgtcaccgg gcgggcgagc gccgaggttg cagccaaggt cgaccccgcg agcgcaagca
     1201 tgagcgccat cacggtggct cgaccggacg gcgcagacgc cgggccgaaa cgcccggacg
     1261 ggaaacggaa acgcatgggc aatctgattc gcagcatcaa cgctttcatt ctaccggctg
     1321 cgatgtcttc ggccca
//
LOCUS       DASUCJ010000653         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_679474, whole genome shotgun sequence.
ACCESSION   DASUCJ010000653 DASUCJ010000000
VERSION     DASUCJ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679474"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFU90_07785"
     CDS             <1..615
                     /locus_tag="VFU90_07785"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5479720.1"
                     /translation="GEIEILLGTQMIAKGLDFPNVTLVGVIDADVGINMPDFRASERS
                     FQLLSQVAGRAGRGPKGGDVVIQTRVPAHHAVRCAVTHDYHAFVRTELPTRVKPPYPP
                     NVRLVNIVCSGLEETATASLALDAAKWLTRLIDARAKDQVTLIGPAPCAIDRIKRRWR
                     WHVLLKSDQPAALTSVVRYFVRRFEAPHAAQLRIAVDRDPVSVL"
     gene            697..>1680
                     /locus_tag="VFU90_07790"
     CDS             697..>1680
                     /locus_tag="VFU90_07790"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479721.1"
                     /translation="MPEFKHPDFSAARFAECPEARFVAAPADGVLPEGFFSTTNLPTY
                     VHIGREWRMPREPRMDGALVLDAGGTLWVKEMRRVAKGELVAVGMAEDGSEGIFVYTE
                     AFLGDAAEGGEFKFMTSEVSREKPIDYALMARLLVEERERGGYPVWVTGPALVHSRAR
                     TDMTWFIQHGFVGALLAGNAVAVHDIEASIFGTTLGMTGSGRATSGGHGLHMRAINRV
                     RAAGSIEAAVRQGIITDGIMHACVTSGVPYVLCGSIRDDGPLPSVIADSIDCQDAMKV
                     HTTKATMAILIATALHAIATGNMLPAFVTDPDGTLRELPTICVDSSEFVVSK"
BASE COUNT          272 a          590 c          546 g          272 t
ORIGIN      
        1 ggcgagatcg agattctgct cggcacccag atgatcgcca aaggactcga ctttccgaac
       61 gtcacgctcg tgggcgtcat cgatgccgac gtcggcatca acatgccgga ttttcgtgca
      121 tcggagcgca gcttccagct gctgagccag gtggccggcc gcgccggtcg cggccccaag
      181 ggcggcgacg tcgtcattca aacccgagtc cccgcgcacc acgccgtgcg ctgcgccgtg
      241 acgcacgact atcacgcgtt cgtgcgcacc gagctgccca cccgcgtcaa gccgccgtat
      301 ccgccgaacg tgcgactcgt gaacatcgtg tgcagcggac tcgaggagac cgcgacggcc
      361 tcactggcgt tggacgcggc aaagtggctc acgcgattga tcgatgcgcg cgccaaagat
      421 caggtcacgc tgatcgggcc ggccccgtgc gccatcgacc ggatcaagcg ccgctggcgc
      481 tggcacgtgc tgctcaagag cgaccagccc gcggcgctca cgtcggtcgt gcggtatttc
      541 gtccgtcgct tcgaggcgcc gcacgcggcg cagttgcgaa tcgcagtcga ccgggacccg
      601 gtatccgtcc tctgaccccg gtcctggcta accaacgccg atgaccggcg gcctcgtcca
      661 catgccggaa aagagctcgt cctagtatct ttctgcgtgc ccgagttcaa acatcccgat
      721 ttcagcgccg cgcgcttcgc cgagtgtccc gaggcccgct tcgtggcggc tcccgcggat
      781 ggtgtcctgc ccgaagggtt tttctcgacc accaaccttc ccacctacgt ccacatcgga
      841 cgcgagtggc gcatgccgcg cgagccgcgc atggacggcg cgctggtgct cgacgccggt
      901 ggcacgctct gggtgaagga gatgcgccgc gtcgccaagg gcgagctcgt cgccgtcggg
      961 atggccgagg acggttcgga aggcatcttc gtgtacaccg aggccttcct gggcgacgcc
     1021 gcggaaggcg gcgagttcaa gttcatgacc agcgaggtgt cgcgcgagaa gccgatcgac
     1081 tacgcgctca tggcgcgtct gttagtcgaa gagcgcgagc gcggcggcta cccggtctgg
     1141 gtcaccggtc ccgcgctcgt gcactcgcgc gcgcgcaccg acatgacgtg gttcatccag
     1201 cacggcttcg tcggcgcgct cctggccggc aacgccgtcg cggtgcacga catcgaagcg
     1261 tcgatcttcg gcaccacgtt aggcatgacc ggcagcggcc gcgcgacgag cggcggccac
     1321 ggcctccaca tgcgcgccat caaccgcgtg cgcgcggccg gctcgatcga ggcagccgtg
     1381 cggcagggga tcatcaccga cggcatcatg cacgcgtgcg tgaccagcgg cgtcccctac
     1441 gtgctgtgcg gttccatccg cgacgacggc cccttgccca gcgtgatcgc cgattcgatc
     1501 gactgccagg acgcgatgaa ggtgcacacc accaaagcga cgatggcgat cctcatcgcc
     1561 acggcgctcc acgcgatcgc taccggcaac atgctcccgg cgttcgttac cgatcccgac
     1621 ggcacgctcc gcgagctgcc gacgatctgt gtggactcat cggagttcgt ggtcagcaag
//
LOCUS       DASUCJ010000654         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1336702, whole genome shotgun sequence.
ACCESSION   DASUCJ010000654 DASUCJ010000000
VERSION     DASUCJ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336702"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFU90_07795"
     CDS             complement(<1..516)
                     /locus_tag="VFU90_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479722.1"
                     /translation="MTMSKLALAPLASVCMCIAAASLGAQQIPGTAAKNAARRVVAAT
                     NAHIDAMQNTDGPAPKRPTATPTRVADATHNRAPSDRSTGGVSPAAASARTHGRAAAD
                     HASHAATKGRDTVTIGAQGTSGDGTTSISERGGKNEIALTREIFSYNADGRRDPFVSL
                     IESGELRPMVSD"
     gene            complement(513..>1114)
                     /gene="pilO"
                     /locus_tag="VFU90_07800"
     CDS             complement(513..>1114)
                     /gene="pilO"
                     /locus_tag="VFU90_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU5479723.1"
                     /translation="LVLVCVLAIAAIGLYWYMVYSPRSDALTKQQAHVEALTAMNQRA
                     KSEMSKGNLSELRAQLATYQQNLSLIRTLVPAGNEVPALLEQVSTAARHAGLDLASVD
                     PQPVQEGSNYDTYRYGMAMVGGYHQLAEFFANVGSLQRIVLPVNVQLTLSTNANAKAA
                     RTDPNAAVIEARFQLQTFVTHNPAEDADDQPPPKKGAKS"
BASE COUNT          159 a          357 c          376 g          222 t
ORIGIN      
        1 gtcggagacc atcggtctga gctcaccgga ctcgatgagc gacacgaatg gatcccgccg
       61 cccgtcggcg ttgtacgaga atatttcgcg cgtgagcgcg atttcgttct tcccgccgcg
      121 ctcggagatc gacgtggttc cgtctccgct cgtgccttgt gcgccgatgg tcaccgtgtc
      181 gcggcctttt gttgcagcgt gggacgcgtg atcggccgcc gcgcgaccgt gcgtccgcgc
      241 cgatgccgcg gccggggaga caccaccggt cgaccggtcg gacggcgccc gattgtgggt
      301 cgcgtccgcg acacgcgtcg gggtcgcggt cggccgttta ggcgccgggc cgtccgtgtt
      361 ctgcatcgca tcgatgtgcg cgttcgtcgc cgcgaccacg cggcgcgctg cgtttttcgc
      421 ggcggtgcct ggaatctgct gtgcgcctaa cgacgccgcg gcgatgcaca tgcacaccga
      481 cgccagcggg gcgagtgcga gtttgctcat ggtcatgatt tcgccccctt cttcggggga
      541 ggctgatcgt ccgcgtcttc cgcgggattg tgcgtcacga acgtctgcag ctggaagcgc
      601 gcttcgatga ccgcagcgtt cgggtccgtc cgcgcagcct tcgcattggc gttcgtcgac
      661 agcgtcaact ggacattcac cggcagtacg atacgctgga gcgatccgac attcgcgaag
      721 aactcggcga gctggtgata tccgcccacc atcgccatgc cgtagcggta cgtgtcgtag
      781 ttgctgcctt cctgcaccgg ctgcggatcg acggaagcaa ggtcgagccc cgcgtgccgt
      841 gcagcggtcg agacttgctc gaggagcgcc ggcacttcgt ttccggccgg cacgagcgtt
      901 cgaatgagcg acaggttctg ttgatacgtg gccagttggg cacgcagctc gctcaagttg
      961 cctttactca tctcggactt ggcgcgctgg ttcatggcgg tgagcgcctc gacgtgcgct
     1021 tgctgcttcg tgagcgcatc cgaccgcggc gagtagacca tgtaccagta gagaccgatc
     1081 gctgcgatcg cgagcacgca cacgagcacg agcg
//
LOCUS       DASUCJ010000655         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1168656, whole genome shotgun sequence.
ACCESSION   DASUCJ010000655 DASUCJ010000000
VERSION     DASUCJ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168656"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFU90_07805"
     CDS             <1..1124
                     /locus_tag="VFU90_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5479724.1"
                     /translation="TSTAWLAVDQNPERFVRGVATLKQPYVVIPELSALLSPFAEYRD
                     DFRDRSWAIGSDATLLYRLGPILLLSGKYSYSSRHVLQYRNGDFSSGRIDFLTLLAQN
                     ALLGSLHTTVQRSTLTFDATLGTLDNVNAPRNGIVLRPSIELTAGSALNTVEYTRLDV
                     TGAAYHPINRRVGIAIRFSAGRLYPRGKSLLLGGSDSTLKFLELRDVAFTAGGSADVR
                     GWSSGLLGPKFPDLRFDIRGTDTTAVASGYVPIGGLARIAGTFELRMPFPGLGDNWGT
                     HVFVDGGRVWTPDERFKTSFTLPGDDRFFTSVGTGVDYNTVIGAVRVSIGYKLNPSLD
                     DVRDPQKVLDAIIAGTPITAVPTEWWRRFQIHLALGTAF"
BASE COUNT          192 a          411 c          362 g          204 t
ORIGIN      
        1 gcacgtcgac ggcgtggctg gccgtggacc agaaccccga acgcttcgtg cgcggggtgg
       61 cgacgctcaa gcagccgtac gtcgtcatcc ccgaattgtc cgccttgctc agtccgttcg
      121 ccgagtaccg cgacgacttt cgcgaccgct cgtgggccat cggatcggac gccaccctgc
      181 tctatcggtt aggcccgatc ctcctgctgt cgggcaagta cagctactcg tcgcggcacg
      241 tgctccagta tcgcaacggc gacttctcat ccggccgcat cgactttctc acgctgttgg
      301 cgcagaacgc cctgctcggt tcgctgcaca ccacggtcca gcggagcacg ctcacgttcg
      361 acgcgaccct cggtaccctc gacaacgtca acgcgccgcg gaacggcatc gtgctgcgac
      421 caagcatcga gctcacggcc ggcagcgcgc tcaacacggt ggaatacacg cggctcgatg
      481 tcaccggcgc cgcgtaccat ccgatcaacc ggcgcgtcgg catcgcgatc cggttcagcg
      541 ccggccggct gtaccctcgg ggcaagagtc ttctgttagg cggcagcgat tcgacgctca
      601 agttcctcga gctgcgcgac gtcgccttca ccgccggagg ctccgccgac gtgcgcggct
      661 ggagcagcgg actcttgggc cccaaatttc ctgacctgcg cttcgacatc cgcggcaccg
      721 acacgaccgc cgtcgcgagc ggctacgtgc ccatcggcgg cctcgctcgc atcgcgggca
      781 cgttcgagct ccgcatgccg tttcccgggt taggcgacaa ctggggcacg cacgtgttcg
      841 tcgacggcgg ccgcgtatgg acgcccgacg agcgcttcaa gacgagcttc accctgccgg
      901 gcgacgaccg cttcttcacg tcggtgggaa caggagtcga ctacaacacc gtgatcggcg
      961 ccgtgcgggt gtcgatcgga tacaagctca atccgtccct cgacgacgtt cgcgacccgc
     1021 agaaagtgct cgacgccatc attgccggaa cgccgatcac ggccgtcccc acggagtggt
     1081 ggcgacgatt ccagattcac ctcgcgctcg gcacggcttt ttagtgagcg ttggagttgc
     1141 tgtttccggc aacagcggga agggggaag
//
LOCUS       DASUCJ010000656         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_679495, whole genome shotgun sequence.
ACCESSION   DASUCJ010000656 DASUCJ010000000
VERSION     DASUCJ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679495"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1163
                     /locus_tag="VFU90_07810"
     CDS             <1..>1163
                     /locus_tag="VFU90_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479725.1"
                     /translation="HVLAKSDTAAIGDPIVQPGLIDAGLTPDTKCDATAATTVANLSQ
                     FVNLEAESSASSANIDAAIAQVVPGQVDPAGNILFLGGAADSNGVPLPGAPNAGGGIP
                     ATLGLEVAKSGRSTGLTCSTVLSVSVDTSVEYNKSCDGTGAKFTVDYDNQVDVTGGDF
                     AAEGDSGSLIVSQNTADPVALLYAGSDSDVVGNPVSQVLAFFSSGGSPTTFVGGGQHA
                     VIGCTLPSGPQSATTRTAVTLAVKTPVVEQSELQRATAARDARASALMAQSAVQAVGV
                     GASLDNPGEPAIEFFVTRGASHAGIPSEVNGIRTRIVEGDLFVKRGAQISAADSATNE
                     EAGAAPRLVYSISAAEMTRAYTVKTAHAKDLMKLAGVQGVGVTSSVDSPGEAA"
BASE COUNT          205 a          262 c          479 g          217 t
ORIGIN      
        1 tcatgtgctg gcgaagagcg acacagcggc gatcggcgat ccgatcgtgc agccgggatt
       61 gatcgatgcg ggactgacgc cggatacgaa atgcgatgct acggcggcga ctacggtggc
      121 gaatctgtcg cagtttgtga acctggaggc ggagagttcg gcgtcgtcgg cgaatatcga
      181 cgcggcgatc gcgcaggttg tgccggggca ggtcgatccg gcggggaata ttttgttttt
      241 gggcggcgcg gcagattcga acggcgtgcc gctgccggga gcgccgaatg cgggcggggg
      301 aattcctgcg acgctggggt tggaagtggc gaagagcggg aggtcaacgg gattgacgtg
      361 ttcgacggtg ttgtcggtga gcgtggatac cagcgtcgag tacaacaaga gctgcgatgg
      421 gaccggcgcg aagttcacgg tggactacga caatcaggtg gacgtgaccg gcggggattt
      481 tgcggcagag ggagattcgg gatcactgat cgtgtcgcag aacacggctg atcctgtggc
      541 gctgttgtat gccggatcgg attcggatgt cgtggggaat ccggtgtcgc aggtgttggc
      601 gtttttttcg agcggcggga gtccgacgac gtttgttggc ggcgggcagc acgcggtgat
      661 cgggtgcacg ttgccgagcg ggccgcagtc ggcgactacg cgaacggctg tgacgttggc
      721 ggtgaagacg ccggtggtgg agcagagcga gttgcaacga gctacggcgg cgcgcgatgc
      781 gcgggcttcg gcattgatgg cgcagtcggc ggtgcaggct gtgggagtcg gggcgagcct
      841 ggacaatccg ggtgagccgg cgatcgaatt ttttgtgacg cgaggagctt cgcacgcggg
      901 cattccgagc gaggtgaacg ggattcggac acggattgtg gaaggcgatt tgttcgtgaa
      961 gcgcggtgcg cagatttcgg cggcggacag cgcgacgaat gaagaggcgg gggcggcacc
     1021 gcggctggtg tattcgattt cggcggccga gatgacgcga gcttacacgg tgaagacggc
     1081 gcacgcgaag gatttgatga agttggcggg cgtgcaagga gttggagtga cgtcgagcgt
     1141 ggattcgccg ggtgaagcgg cgc
//
LOCUS       DASUCJ010000657         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_282095, whole genome shotgun sequence.
ACCESSION   DASUCJ010000657 DASUCJ010000000
VERSION     DASUCJ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282095"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1450
                     /locus_tag="VFU90_07815"
     CDS             62..1450
                     /locus_tag="VFU90_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEU5479726.1"
                     /translation="MRKMSVIAMGLLLTGVATGGRLAAAQTAALPVTDTLQPIGTRDM
                     RAFGQAARGVFLAAFIPYSYNRWVRHVPWAQTTLASWGANLRSGWKWDTDHFLANQFA
                     HPYQGSFYYSAARSSGYSFWHAAPFALAGSAMWEYFGERFPPSLNDMVNTTLGGITLG
                     EATYRLADAVSTTPDGRRPGVLRRVAATVLNPERAFAGATGGAGDPLGARLPAISAHV
                     EVGAQRLVTGAFGALGKGSSYPFVYSSLAYGDPFAGDVRKPFGAFRVDMSIAGGRMAE
                     LRALGFLAVHVLDGTPPTQRQLALAMHYHYYDNRAYQGGGQGFSGGLLSDHDLGHHTR
                     LRTEVWVTGLLLTAIKSDHGPSADSVANETARNYDYGSGVGARFLLLLTHRGRPLLDV
                     AYQTFWVGVLSGTAQNHYYDTWSICSELPLVWNSALGARALMYHRVSNYARYPTVHRS
                     DPQLRIFLATDW"
     gene            complement(1480..>2132)
                     /locus_tag="VFU90_07820"
     CDS             complement(1480..>2132)
                     /locus_tag="VFU90_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEU5479727.1"
                     /translation="TGHTAHSSQIFTKEFGAGAIYEAARVLDQFYRTLSTQRYLTFNP
                     GVVVGGTAVSLDSTQSAGTAAGKTNVVSDTAVVAGDLRTLSPAQLAGAQRSMQAIVRQ
                     NLPGTTAHLEFADGYPPMAPSAGNKRLLAMYDRASRDLGFGPVVAVDPSRAGAADVSF
                     VANEVPMAIDALGLSGHDDHSPQETADLRMLPVQTTRAAVLMYRLTQQGGAHTITP"
BASE COUNT          314 a          700 c          756 g          362 t
ORIGIN      
        1 gtgacgtgcc ggagcgcgtc gggcgccagc ttgttcacga actccgagcg gagaagagat
       61 catgcgcaag atgagcgtca tcgcaatggg tctgttgctc acgggtgtcg cgaccggtgg
      121 ccggctcgca gcggcgcaga cggctgcgct gcccgtcacc gacacgctgc agccgatcgg
      181 gacgcgcgac atgcgtgcat tcggccaggc ggcgcgcggc gtttttctcg ccgcgttcat
      241 tccgtactcg tacaatcgat gggtgcgcca cgtgccgtgg gcgcagacga cgctcgcctc
      301 gtggggtgcg aacctgcgct cggggtggaa gtgggacacg gatcactttc tcgcgaacca
      361 gttcgcgcac ccgtatcagg ggagcttcta ctacagcgcg gcgcggagca gcggctattc
      421 gttctggcac gccgcgccct tcgcgttggc cgggagcgcg atgtgggagt acttcggcga
      481 gcgctttccg ccgtcgctca acgacatggt gaacacgaca ctcggcggca tcacgttagg
      541 cgaggccacc taccggttgg cggacgccgt atcgacgaca cccgacgggc gtcgtcccgg
      601 agtgttgcgc cgcgtggcgg cgaccgtgct caatcccgag cgtgcgttcg ccggcgcaac
      661 cgggggcgcc ggcgatccgt taggcgccag gctgccggcg atcagcgcgc acgtagaagt
      721 cggcgcccaa cgcctggtca ccggtgcgtt cggcgcgctc gggaagggct cgagctatcc
      781 gttcgtgtac tcgtcgctgg cctatggcga cccattcgcg ggagacgtga ggaagccctt
      841 tggtgcgttt cgcgtggaca tgtcgatcgc cggaggccgg atggccgagc tccgcgcgct
      901 cggctttctc gcggttcacg tcctggatgg tacgccaccg acgcagcgcc aactcgcgct
      961 cgccatgcat taccactact acgacaaccg ggcgtatcaa ggcggcggcc agggattcag
     1021 cggcggcctt ctcagcgacc acgacctcgg gcaccacact cgcttgcgca ccgaggtctg
     1081 ggttaccggg ttgctgctca cggcgatcaa atcggatcac gggccgagtg ccgattcggt
     1141 cgcgaacgag acggcgcgca actacgacta cggttccggc gtcggcgcgc ggtttctttt
     1201 gctgctgacg caccgcggcc gcccgcttct ggacgtcgcg tatcagacgt tctgggtcgg
     1261 cgtgctgagc ggcacggcgc agaatcatta ttacgacacg tggagcatct gcagcgagct
     1321 tccgttagtc tggaactcgg cgctcggggc tcgcgcgttg atgtaccacc gcgtcagcaa
     1381 ctacgcgcgc tacccgacgg tgcaccgcag cgacccgcag ctccggatat tcctggccac
     1441 cgactggtga gccgcgcggg gccgtggccg gcccggccgt cacggcgtga tcgtgtgagc
     1501 gccgccctgc tgcgttaggc gatacatcag gaccgcggcg cgcgtggtct ggaccgggag
     1561 catgcggagg tcggccgtct cctgcggcga atggtcgtcg tgtccggaca ggccgagcgc
     1621 atcgatcgcc atgggcacct cgttggcgac gaacgagacg tccgcggcgc cggcgcgcga
     1681 cggatcgacc gccacgaccg gtccgaaccc caagtcgcgg ctggcgcggt cgtacatcgc
     1741 gaggaggcgt ttgttgccgg cgctgggcgc catgggcggg tagccgtcgg cgaactcgag
     1801 atgcgccgtg gtgccgggga gattctgcct aacgatcgcc tgcatcgatc gttgggcgcc
     1861 ggcgagctgc gcggggctca aggtcctgag atcgccggcc accaccgccg tatcggacac
     1921 gacgttggtc tttccggcgg ccgtcccggc gctctgcgtc gagtcgagcg agacggccgt
     1981 gccgccgacc acgacgccgg ggttgaacgt gaggtagcgc tgcgtcgaca gcgtccggta
     2041 gaactggtcg agcacgcgtg ccgcctcgta gatcgcgccg gcgccgaact ccttggtgaa
     2101 gatctgcgac gagtgcgccg tgtggcccgt cg
//
LOCUS       DASUCJ010000658         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1428056, whole genome shotgun sequence.
ACCESSION   DASUCJ010000658 DASUCJ010000000
VERSION     DASUCJ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428056"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFU90_07825"
     CDS             complement(<1..1236)
                     /locus_tag="VFU90_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479728.1"
                     /translation="MQETAPRSRPFCDLVMKGGVASGIVYPDAIYEISRSFDLKSIGG
                     TSAGAIAAALAAAAQYRRLRAAGGDAEAGFERVRAIPDTLAAEGRLFKLFAPNRSTRA
                     LFAIAVDLFSPATPLAVKTVCLLRAYPLHALAGLLPGALYAFATAAGRAPVPILHYAV
                     ALVVALLGAIAACVFGLLWDMLRKLPGNCYGFVTGIDDADPEGTLALDAWLAAELEET
                     AGLEAGKVPLTFGMLWNPQAAPGPCSIDTPPPGDARAVNLQMVTTCLTEGRPYQFPTR
                     TARFYFKPAEFAKFFPAHVVQWLRDCSRPDDGALEGLVALPPIGDLPVIVAVRMSLAF
                     PVLLSAVPLYAIDFTDLAPQQPQPVWFSDGGLTSNFPIQMFDSPIPRWPTLALNLGPF
                     GPRTPADGVATAGSTGSGRL"
     gene            1303..>2176
                     /locus_tag="VFU90_07830"
     CDS             1303..>2176
                     /locus_tag="VFU90_07830"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEU5479729.1"
                     /translation="MSDATLYPYVLDPKLAPAIWGGGELVADYGKRGDPNAKLGESWE
                     CWDADKVTNGALAGATVADLRARLGAELLGDLDPQRIFPVLTKIITAHDWLSVQVHPD
                     DAYAQRVEHQPNGKTECWYVFAAEPGAQLVLGWTRDTSRAEYERRVADGTLGDILRKI
                     PVRAGDSIYIPAGMVHAIGAGVVLFETQQASDLTYRMFDWNRVGADGKPRELHVRKAA
                     DVLDYAAGTAGPLVQIAYRFEGLERLALIADRHFVVERIVATSDPASLATNGRPLAIM
                     SLDAPLNVASAGVSV"
BASE COUNT          398 a          749 c          712 g          317 t
ORIGIN      
        1 caagcgtccg cttcccgtcg atccggccgt cgcgacgccg tcggcgggcg tgcgcggccc
       61 gaacggaccg agattcagcg ccagcgtcgg ccagcgaggg atcggcgaat cgaacatttg
      121 aattggaaag ttgctcgtta gcccgccgtc ggaaaaccac accggctgcg gctgttgcgg
      181 tgccagatcc gtaaaatcga tcgcgtacag cggaacggcg ctgagcagca ccggaaacgc
      241 caggctcatg cgcacggcga cgatcaccgg taaatcgccg atcggcggca acgcgacgag
      301 accctcgagc gcgccgtcgt cgggacgcga gcagtcgcgc agccattgca cgacgtgcgc
      361 ggggaagaat ttcgcaaact ccgccggctt gaagtagaac cgcgccgtcc gcgtcggaaa
      421 ctgatacgga cgcccctcgg tcagacacgt cgtcaccatt tgaagattca ccgcacgcgc
      481 gtcgccgggc ggaggcgtgt cgatcgagca cgggccgggc gcggcctgcg gattccacag
      541 catgccgaac gtcagcggga ccttccccgc ttccaagccg gccgtttctt ccagctcggc
      601 ggcgagccac gcatccagcg cgagcgttcc ctcgggatcc gcatcgtcga taccggtaac
      661 gaacccgtag cagttgcccg gcagcttgcg cagcatatcc cacagcagcc cgaaaacgca
      721 cgccgcgatt gcgcccagca gcgcgacgac gagcgcgacc gcgtagtgca ggatcggcac
      781 aggcgcgcgc ccggctgccg tcgcgaaggc gtacagcgcg cccggcagca gccccgccag
      841 cgcgtgcagc ggataggcgc gcaacagaca aacggtcttc accgcaagcg gagtcgccgg
      901 cgagaacagg tcgacggcga tcgcaaacag cgcgcgcgtg gatcgattcg gtgcgaacag
      961 cttaaagaga cggccttccg ccgcaagcgt atccggtatc gcgcgcactc gttcgaagcc
     1021 ggcctccgca tcgccgccgg cggctcgcaa gcgccggtac tgcgccgctg cggcgagcgc
     1081 cgcggcaatc gctcccgccg aagtgccgcc gatgctcttg aggtcgaaac tgcgggaaat
     1141 ctcgtaaatc gcgtccggat agacgattcc gctggcaacg ccgcccttca tcaccaaatc
     1201 gcaaaacggg cgcgaacgcg gtgcggtctc ttgcatacct tccatgcgtc cttggaccgg
     1261 tgcacgcgag gcggcggttt cgcgacagcg aagtgaggca cgatgagcga tgcgacgttg
     1321 tatccgtacg tcctcgaccc gaaattggcg ccggcaattt ggggcggcgg cgagctggtg
     1381 gccgattacg gtaaacgcgg cgacccaaac gccaaactcg gcgagtcgtg ggaatgctgg
     1441 gacgccgaca aagtcaccaa cggcgcgctc gcgggcgcga cggtggcgga cctgcgcgcg
     1501 cggcttggcg ccgagctcct cggcgacctc gacccgcagc gcatctttcc ggtgctcacc
     1561 aagatcatca ccgcgcacga ttggctttcc gtgcaggtcc atcccgacga cgcgtacgcg
     1621 caacgggtcg agcatcagcc caacggcaag acggagtgct ggtacgtctt cgccgccgag
     1681 ccgggcgcgc agctggtgct cggctggacg cgcgacacgt cgcgcgccga atacgaacgc
     1741 cgcgtcgccg acggcacgct cggcgatatc ctgcgcaaga ttccggtcag ggcgggcgat
     1801 tcgatttaca ttccggccgg catggtgcac gcaatcggag ccggcgtggt gctgttcgaa
     1861 acgcagcaag cctccgacct gacctatcgc atgttcgatt ggaaccgcgt cggcgccgac
     1921 ggcaagccgc gcgagctgca cgttcgcaaa gcggccgacg tgctcgacta cgccgccgga
     1981 acggccggac cgctcgtaca aatagcctat cgcttcgagg gcctcgagcg gttggcccta
     2041 atcgccgacc ggcacttcgt cgtcgagcgc atcgtcgcca cctccgatcc ggcgtcgctg
     2101 gcgaccaacg gacggccgct cgcgattatg tcgctggacg cgccgctaaa cgtcgcctcc
     2161 gccggcgtct cggtcg
//
LOCUS       DASUCJ010000659         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_541653, whole genome shotgun sequence.
ACCESSION   DASUCJ010000659 DASUCJ010000000
VERSION     DASUCJ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541653"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU90_07835"
     CDS             <1..295
                     /locus_tag="VFU90_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479730.1"
                     /translation="LAGRLAGADVALLDGTFFTDDEPQRFGLGDRTAAQMGHAPISGK
                     AGTLGRFGGLPCRHKVYTHINNTNPILVEDSPERRTVELAGVVVGADGMTFQI"
     gene            298..1047
                     /gene="pqqC"
                     /locus_tag="VFU90_07840"
     CDS             298..1047
                     /gene="pqqC"
                     /locus_tag="VFU90_07840"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEU5479731.1"
                     /translation="MAASSFTTMSAPPPPPIPRLERPLPPNELADALRAFHSSYYVEH
                     PFHQLMHDGQLTQRQLQGWVANRLCYQRAVPRKDGAILSNCPVPAVRRQWIQRLIDHD
                     GTREGEGGIEKWIQLGIGLGLSREEMEDERYVLPGVRFATEAYVTFCKTKPWVDAVAA
                     SLTELFAPDIMRRRLASMPKNYPWIKPEAYAYFESRLTQAPRDSAEGLAIVTTYCTTV
                     ETQQRAFEALRFKIEMLWAMIDTIHHAYREE"
     gene            1050..1349
                     /gene="pqqD"
                     /locus_tag="VFU90_07845"
     CDS             1050..1349
                     /gene="pqqD"
                     /locus_tag="VFU90_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEU5479732.1"
                     /translation="MGFGGGTRPRLWRFARLDFDPVRQRRVLLYPEGAILLNDTAAEI
                     LALCDGERTIDAIAATLGERYGADVRADVLDYLDKLGERALVRDAAAPPSVPTSP"
     gene            1349..>2259
                     /locus_tag="VFU90_07850"
     CDS             1349..>2259
                     /locus_tag="VFU90_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5479733.1"
                     /translation="MRFRLRLCLAVCASLVFGAGTAAAQRPRSAAPAAAGAPAALPAA
                     AAGMRATLEKVIHKTVLPNGLEVIVIENHGVPLATVEIDVRNGAFTQTPEYEGLAHMY
                     EHMFFKSNASYPEPEQFVQRAAELGAVFNGSTREEVVNYYITLPSDSVAAGLSFLAAA
                     FRAPLFRPDELEAERQVVLGEYDRQESSPFFKFTEDMNHLLWRSQYSRKNMIGNRDVI
                     LHVTPAQMREIEHRYYVPNNAALIVAGDVRPDSVFALAQRTFGSIPRGPDPFTKYPIP
                     DVPPLPHDEAEIVEQPVSTVFVQEQWLG"
BASE COUNT          376 a          801 c          732 g          350 t
ORIGIN      
        1 cctcgccggc cgtctcgcgg gcgcggacgt tgcgctcctc gacggcacct ttttcacgga
       61 cgacgagccg caacgctttg gcctgggcga ccgcaccgcg gcgcagatgg gacacgcgcc
      121 catcagcggc aaggcgggca ccctgggccg cttcggaggg ctcccttgcc gtcacaaggt
      181 gtacacgcac atcaacaata cgaacccgat cctggtcgag gattctccgg aacgccgcac
      241 cgtggagctc gcgggcgtcg tcgtgggagc ggacgggatg acatttcaga tctgaccatg
      301 gccgcttcgt cattcaccac catgtcggcg ccgccgccgc cgccgatccc gcggctggag
      361 cggccgcttc cgcctaacga actggccgac gcgctgcgcg ccttccattc gtcgtattac
      421 gtcgagcatc cgttccatca gttgatgcac gacggccagt tgacgcagcg gcagctgcaa
      481 gggtgggtcg ccaaccgcct gtgctaccag cgggcggtgc cgcgcaagga cggggcgatt
      541 ctctcgaact gcccggtccc cgcggtccgc cggcagtgga tccaacgcct catcgaccac
      601 gacgggacgc gcgagggcga aggcggcatc gaaaaatgga tccagttagg catcggactg
      661 ggcctctcgc gcgaggagat ggaagatgag cgctacgtcc tgcccggcgt gcgcttcgcc
      721 accgaggcgt acgtcacctt ctgcaagacc aagccgtggg tcgatgcggt ggccgcgtca
      781 ttgacggagc tgttcgcgcc ggacatcatg cgccgccgtc tcgcgtcgat gccgaagaac
      841 tatccgtgga tcaagcctga ggcctacgcg tacttcgaga gccgcctgac gcaggcgccg
      901 cgcgacagcg ccgaagggct ggcgatcgtg accacgtact gcaccaccgt cgagacgcag
      961 cagcgcgcgt tcgaggcgct gcggttcaag atcgagatgc tgtgggccat gatcgacacg
     1021 atccaccacg cctaccgcga ggagtagccg tgggattcgg cggtggcacg cggcccagac
     1081 tctggcggtt cgcccgcctc gacttcgacc cggtgcggca gcgtcgcgtg ctgctctatc
     1141 ccgaaggggc gatcctgctc aacgacacgg cggccgagat cctcgcgctg tgcgacggcg
     1201 agcggacgat cgacgcgatc gcggcgacgt tgggcgagcg ctacggcgcc gacgtgcggg
     1261 ccgacgtgct cgactacctc gacaagttgg gcgagcgcgc gctcgtccgc gacgccgccg
     1321 cgccaccctc ggtcccgacc tccccgtgat gcggttccgc ctccgcctgt gcctcgcggt
     1381 ctgcgcgtcg ctcgtcttcg gcgccggaac cgcggctgcc caacgaccgc gttccgcggc
     1441 cccggcggcg gcgggcgcgc ccgcggcgct gccggcggcc gcggccggca tgcgcgccac
     1501 gctcgagaaa gtgatccaca agaccgtgct gcctaacggg ctcgaggtga tcgtcatcga
     1561 aaaccacggc gtcccgctgg cgacggtcga gatcgatgtg cggaacggcg cgttcacgca
     1621 gacccccgag tacgaggggc tcgcgcacat gtacgagcac atgttcttca agtcgaacgc
     1681 ctcctacccc gaacccgagc agttcgtcca acgcgcggcc gagctcggcg ccgtgttcaa
     1741 cggcagcacg cgcgaagagg tggtgaacta ctacatcacg ctgccatcgg acagcgtcgc
     1801 ggccggcctg tcgtttctcg ccgccgcgtt tcgcgcgccg ctctttcgcc cggatgagct
     1861 cgaggccgaa cgccaggtcg tgttaggcga atacgaccgc caggaatcga gcccgttctt
     1921 caagttcacc gaagacatga accatctctt gtggcgctcg cagtacagcc gcaagaacat
     1981 gatcggcaat cgcgacgtca ttctgcacgt caccccggcg cagatgcgcg agatcgagca
     2041 ccgctactac gtccccaaca acgcggcgct catcgtggcc ggcgatgtcc gacccgacag
     2101 cgtgttcgcg ctcgcccaac gcaccttcgg cagcattccg cgcggccccg acccgttcac
     2161 caaatacccg atccccgacg tcccgcccct gccgcacgac gaagccgaga tcgtcgagca
     2221 gccggtgagc acggtgttcg tccaggagca gtggttagg
//
LOCUS       DASUCJ010000660         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1306060, whole genome shotgun sequence.
ACCESSION   DASUCJ010000660 DASUCJ010000000
VERSION     DASUCJ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306060"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFU90_07855"
     CDS             <1..312
                     /locus_tag="VFU90_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5479734.1"
                     /translation="AAARGPLWLDTAKFPIARFRSTAITVDGGDRYRVTGRLTLKGIT
                     RDCVVPIRVTTDGAGNRVATGTFTLKRLEYHVGEGEWADPAAVADDIAVRIRLVLPAS
                     G"
     gene            363..965
                     /locus_tag="VFU90_07860"
     CDS             363..965
                     /locus_tag="VFU90_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5479735.1"
                     /translation="MNLRSLLAVPLAGVAGVAGVPGVALASPEVYVLDPVHSQVSFEA
                     RHMGMTTQRGRFMRSSATVTLDTAAKKGSVDVTIEAASVRTYDPHLDAIVKGPKFFDV
                     ERFPTITYKSTKVVFDGDRVVGVDGELTMLGVTHPVTLTIVDYVCGPQPFNRKPMCGG
                     DATATIRRSEWGMTANLPLAPADEIKLDIPFEGYRQAPPG"
     gene            complement(980..>1551)
                     /locus_tag="VFU90_07865"
     CDS             complement(980..>1551)
                     /locus_tag="VFU90_07865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5479736.1"
                     /translation="TATEIIRAGTVHVWGDLEWRTLAAPGHDTGALVFFNDAHGILIS
                     GDALWRNGYGFVMPAAFDAAALPATRATLEMLATLGARCVIPGHGEPFADVDDAIERA
                     LRRTAAFEADPLRLVWHAMKVMLVFSLLDRERMPASAIPGWVEHTGIIRDFNAQYLGL
                     APDVLAERLAIELVKTGALRRDGSDLVPA"
BASE COUNT          257 a          504 c          536 g          254 t
ORIGIN      
        1 gccgcagcgc gcggaccgct gtggctcgat accgcgaaat tcccgatcgc tcgctttcgc
       61 tcgaccgcga tcaccgtgga tggcggcgac cgctatcgcg tcaccggtcg cctgacgctc
      121 aagggaatca cccgcgactg cgtcgtacct atccgggtca cgaccgatgg cgccggcaat
      181 cgtgtcgcaa ccggcacctt tacgctgaag cggctcgagt accatgtcgg cgaaggcgaa
      241 tgggcggatc ctgcggcggt cgccgacgac atcgcggtgc ggatcagact ggtgttgccc
      301 gcctcgggat gacggcccgg cgacgcgtat cgcagttcat cgctgccagg aggactttcg
      361 acatgaatct acgcagcctg ctcgcagtcc cgcttgccgg cgttgcgggc gttgccggtg
      421 ttcccggcgt cgcgctcgcc tcgcccgagg tctatgtgct cgatcccgtg cattcgcagg
      481 tctcgttcga ggcgcggcat atggggatga cgacgcagcg cggccgcttc atgaggtcgt
      541 cggcgacggt cacgctcgat accgcggcga agaagggttc tgtcgacgtg acgatcgagg
      601 cggcgtcggt gcgcacctac gatccgcacc tcgacgcgat cgtcaagggt ccgaaattct
      661 tcgacgtcga gaggtttccg acgatcacct acaaatcgac gaaggtcgtg ttcgacggcg
      721 atcgcgtcgt cggcgtcgac ggtgagctca cgatgctggg agtgacgcac ccggtgacgc
      781 tcacgatcgt ggattacgtc tgtggcccgc agccgttcaa caggaagccg atgtgtggcg
      841 gtgacgccac cgcgacgatc cgacgctcgg aatgggggat gacggcgaac ctcccgctcg
      901 cgcccgccga cgagatcaag ctcgacattc cattcgaagg ctatcgtcag gcgccgccgg
      961 gctgacgcga agcgtcgcgt cacgctggaa cgaggtcgct gccgtcacgg cgcaatgcgc
     1021 cggtcttgac gagctcgatc gcaaggcgct cggcgagcac gtccggcgcg agccccaggt
     1081 actgcgcgtt gaaatcgcgg atgatgccgg tgtgctcgac ccagccggga atggcggagg
     1141 ccggcatccg ctcccggtcg agaagcgaga acacgagcat caccttcatt gcgtgccaga
     1201 cgaggcgcag cgggtcggct tcgaacgcgg ccgtgcgacg cagtgcgcgc tcgatcgcgt
     1261 cgtcgacgtc ggcgaacggc tcgccgtggc ccgggatcac gcagcgcgca ccgagcgtgg
     1321 cgagcatttc gagcgtcgcg cgcgtcgcgg ggagcgccgc ggcgtcgaag gccgcaggca
     1381 tcacgaagcc gtagccgttg cgccagagcg catcgccgga gatcaggatg ccgtgcgcgt
     1441 cattgaagaa caccagcgca cccgtgtcgt ggccgggtgc ggcaagggtg cgccattcga
     1501 gatcgcccca gacgtgtacg gtgccggccc gaatgatctc cgtcgccgtg a
//
LOCUS       DASUCJ010000661         1410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_327661, whole genome shotgun sequence.
ACCESSION   DASUCJ010000661 DASUCJ010000000
VERSION     DASUCJ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327661"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFU90_07870"
     CDS             complement(<1..442)
                     /locus_tag="VFU90_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5479737.1"
                     /translation="MDWTLLAVLLVVGGLVGFLAGLLGIGGGLSMIPLLTIVFSVRAF
                     PPGHVVHMAVATSTATMMFTAASSAHAHARRGGVLWGVVAAMAPGIVLGSLIGPQISA
                     ILPGRVLAAVLGAFTWTAATRMTAARASRPGRTLPGRSSLFGVAA"
     gene            complement(590..1057)
                     /locus_tag="VFU90_07875"
     CDS             complement(590..1057)
                     /locus_tag="VFU90_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU5479738.1"
                     /translation="MRHWLMKSEPDEFSIDDLAKAPKRTTAWFGVRNYQARNYMRDDM
                     RVGDLAFFYHSSCPEPGIAGIVEVSKRAYPDATQFDPESDYYDPNATREAPRWFNVEV
                     TLIRKFPLVGVAALRVTPGLEEMVTLRRGNRLSITPVTPAEWKIVERLAAASR"
     gene            complement(1060..1305)
                     /locus_tag="VFU90_07880"
     CDS             complement(1060..1305)
                     /locus_tag="VFU90_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEU5479739.1"
                     /translation="MLNDHWLGPKNAYLCGAEITIADYFGAGLVSLGEVIGIDFAAYP
                     NIQRWLGNMKKLKTWNQVNEVFNGFVAGNRGKEFVRL"
BASE COUNT          239 a          478 c          475 g          218 t
ORIGIN      
        1 cggccgcgac gccgaacagc gacgatcttc ccggaagcgt gcgtccgggc cgcgaagcac
       61 gcgcggcagt catccgcgtt gctgcagtcc aggtgaacgc tcccagcacg gcggcaagga
      121 cgcggcccgg caggatcgcc gagatctgcg gtccgatgag cgagcccagc acgatgccgg
      181 gagccatcgc ggcgacgaca ccccagagca cgccgcctcg tcgcgcgtgc gcatgcgccg
      241 acgacgcggc ggtgaacatc atcgtcgcgg tcgacgtcgc gacggccatg tgaacgacgt
      301 gaccgggagg gaaggcccgt acgctgaaca cgatcgtcag gagcgggatc atcgacagcc
      361 cgccgccgat gccgaggaga cccgcgagga agccgaccag gccgccgacc acgagcagca
      421 ccgcgagcag cgtccaatcc atgggctcca agtatcgcag gaaggctgtt gccttccgga
      481 ccgatccgtg acggcacgcc gccggcgggc ccgtcgcatc cggtcgaccg gacggaccgc
      541 cgcgcccatt gcggacatgg gcgcgtacgg gttgtgaacg cgatcgcagt tatcggctcg
      601 ccgccgcgag acgttcgacg atcttccatt cggccggcgt gaccggcgtg atcgacaggc
      661 ggttgccccg gcgcagcgtg accatctcct cgaggccggg cgtgacgcgt agcgccgcga
      721 cgccgacgag cgggaacttt cggatgagcg ttacttcgac gttgaaccag cgcggagcct
      781 cgcgcgtcgc gttcgggtcg tagtagtcgc tttccgggtc gaactgcgtc gcgtccgggt
      841 acgcgcgctt cgacacttcg acgatgcccg cgatgccggg ctcggggcag ctcgagtggt
      901 agaagaaggc aagatcgccg acgcgcatgt cgtcgcgcat gtagttgcgc gcctggtaat
      961 tgcgcacgcc gaaccacgcc gtcgtccgct tcggcgcctt cgcgagatcg tcgatcgaga
     1021 actcgtcggg ctccgatttc atcagccagt ggcgcatcgc tacagccgca cgaactcctt
     1081 gccgcgatta ccggcgacga agccgttgaa gacctcgttg acctggttcc aggtcttcag
     1141 cttcttcata ttgccgagcc accgctggat attcgggtat gcggcgaagt cgatcccgat
     1201 gacctcgccg agcgacacca gccccgcacc gaaatagtcg gcgatggtga tctcggcacc
     1261 gcagagatac gcgttcttcg gcccgagcca atgatcgttc agcacctgca gtcagcgccg
     1321 tgcgccgtcc tggccccagg cgatcgcgcc ggcgtgcgcc tcgtcgctgc gccgcttgtg
     1381 gttgggaagg atccgcgggt agacgaggcc
//
LOCUS       DASUCJ010000662         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1107604, whole genome shotgun sequence.
ACCESSION   DASUCJ010000662 DASUCJ010000000
VERSION     DASUCJ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107604"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFU90_07885"
     CDS             <1..633
                     /locus_tag="VFU90_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479740.1"
                     /translation="KLLRSHHELWGLSWVNFIGYVAHEVFPNVWVIYCIAVFGWSTGS
                     IGLTLTLVGVLTAVNQATMIGPVVAKFGERRVLLASLVFAVAGTALMGTDNGIVFLAA
                     IVIVCLPMYNASSQALMTRRVGASEQGELQGALGSIRGISMLIGPGLFTFVFAQFIGP
                     WRAAGLPGAPWYLAALMYAAAFVLAWRVTGKADDVALPAPEPAAVSYVEG"
     gene            complement(639..1007)
                     /locus_tag="VFU90_07890"
     CDS             complement(639..1007)
                     /locus_tag="VFU90_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479741.1"
                     /translation="MIVPPVTVTIVAQAAATAAPASSDAAVAARAQSWLHAMQNGKID
                     RSQLDDKMNATLTDAKLAAVSAKLAPLGEPKSFTLEQKTSHPPYRVYVYKVVFAKMTL
                     LETFTLDASGKIAGFFLNRP"
     gene            complement(1075..>1661)
                     /locus_tag="VFU90_07895"
     CDS             complement(1075..>1661)
                     /locus_tag="VFU90_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5479742.1"
                     /translation="VDAALVEPLACVVKSLRRSGVQPGDSLCIVGLGVMGQMHVLAGR
                     ALGARTFGSDFIAERRALAERHGATAFHPDDAAAVLGEGARVVVCGPGTTAALQGALA
                     CTAPGGTLVMFTPFEPGTHIELDPARFYFGDVRLVASYSCGPDDTRAALRMIADRVVT
                     AAKLNATQVALEEVARAYGDLAAARIVKPIVVFG"
BASE COUNT          253 a          557 c          560 g          291 t
ORIGIN      
        1 aagctgctgc gctcgcatca cgagctgtgg ggtttgagct gggtgaactt catcgggtac
       61 gtcgcgcacg aagtatttcc gaacgtttgg gtcatctatt gcatcgcggt tttcggctgg
      121 agcaccggaa gcatcggtct caccctgacg ctcgtggggg tgctgacggc cgtcaatcaa
      181 gcgacgatga tcggccccgt cgtcgcaaag ttcggcgaac ggcgggtgct gctcgcgagc
      241 ctcgtttttg ccgttgccgg gacggcgctg atgggcaccg acaacggcat cgtattcctc
      301 gcggcaatcg tgatcgtctg tttgccgatg tacaacgcca gctcgcaagc gctgatgacg
      361 cgccgcgtcg gcgcctcgga acagggcgag ctgcagggcg cgctcggctc gattcgcggg
      421 atctcgatgc tgatcgggcc gggactgttc accttcgtgt tcgcacagtt catcggaccg
      481 tggcgagccg cgggcctccc gggcgcaccc tggtatttgg cggcgctgat gtatgcggcc
      541 gcgttcgtcc tggcctggcg cgtcaccggc aaagccgacg acgtcgccct tccggcaccc
      601 gaaccggccg ccgtttcgta cgtcgaggga taacgcgctt acggacgatt gaggaaaaag
      661 ccggcgatct tgccgctcgc gtcgagggtg aacgtctcga ggagcgtcat cttcgcgaat
      721 acgactttat acacgtacac ccggtagggc ggatgactcg ttttctgttc gagcgtaaac
      781 gatttcggtt cgcccaaggg agcgagcttg gcggacaccg cggccagctt cgcatcggtc
      841 aacgtcgcgt tcatcttgtc gtcgagctgc gagcgatcga tcttgccgtt ctgcatcgcg
      901 tgcagccagc tttgggcgcg cgccgcgacg gcggcatcgc tgcttgcggg cgcggccgtg
      961 gcggcggctt gcgcgacgat ggttacggtt accggcggaa cgatcatgcc cggctgttag
     1021 ggccccgcgc gggccgcacc tgtcgggcgg cgagcgcgtc gctcgcctcc gccgctaacc
     1081 gaagacgacg atcggtttga cgattcgcgc tgccgccaga tcgccgtacg cccgcgcgac
     1141 ttcctcgagc gccacctgcg tcgcgttcag tttcgcggcg gtcaccaccc ggtcggcaat
     1201 catccgtaac gctgcgcgcg tatcgtccgg tccgcacgag tagctcgcaa ccagccgcac
     1261 gtcgccaaaa tagaatctcg cggggtccaa ctcgatgtgc gtccccggct cgaacggggt
     1321 gaacatgacg agcgtcccgc cgggcgcggt gcaggcgagc gcaccctgca gcgcggccgt
     1381 cgtgccgggt ccgcacacga ccacgcgcgc gccctcgccg aggaccgcgg ccgcatcgtc
     1441 ggggtgaaag gcggtcgcgc cgtggcgctc cgcgagcgcg cgccgctccg cgatgaaatc
     1501 gctgccgaac gtgcgcgcgc cgagcgcccg ccccgccagc acgtgcatct gccccatcac
     1561 gcccaagccg acgatgcaga gcgagtcgcc gggctggacg ccgctgcggc gaagcgactt
     1621 aacgacgcac gcgagcggct cgaccagcgc ggcgtccacg a
//
LOCUS       DASUCJ010000663         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1352259, whole genome shotgun sequence.
ACCESSION   DASUCJ010000663 DASUCJ010000000
VERSION     DASUCJ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352259"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFU90_07900"
     CDS             <1..502
                     /locus_tag="VFU90_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479743.1"
                     /translation="AQFALLMKIRDKFSATNDAVKTIRYVLYQLDDREKSLPAAQKSA
                     FDAAATPLRDSLQSVADSLYQSQSHASEDPLNYPIRLNNRIGALMGVVSSAWGRPTKQ
                     SYEVYDLLNGLIDVQMRRMKAAMAGVPRVDAVLKAAGLQAIEVAAKEVPGSGNARNDE
                     GGEVEN"
     gene            complement(543..>1054)
                     /locus_tag="VFU90_07905"
     CDS             complement(543..>1054)
                     /locus_tag="VFU90_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479744.1"
                     /translation="SDIILSHIHWDHADGVDLFPNARIRLQQDEYDYYVGPGGAPLHP
                     VIDSLDAAMLFRLNAAGRVVKIPGDSAEVLPGIRAYLGGKHTYASEYLTVPTRSGIVV
                     LASDNVYLYRNLDEHRPIAQTLDSLSNLAAQQRMLKLASKPALIVPGHDPTVFGRFPK
                     IAEGVVRID"
BASE COUNT          190 a          285 c          406 g          173 t
ORIGIN      
        1 cgcgcagttc gcgctgctga tgaagattcg cgacaagttc tctgcgacca acgacgcggt
       61 gaagacgatc cgctacgtgc tgtaccagct cgacgaccgc gagaagagcc tgccggcggc
      121 gcagaagagc gcgttcgacg ccgcggcgac gccgttgcgg gattcgctgc agtcggtggc
      181 cgactcgctg tatcagtcgc agagccacgc gagcgaggat ccgctgaatt atccgatccg
      241 gctgaacaat cggatcggcg cgctgatggg cgtggtatcg agtgcgtggg gccggccgac
      301 gaagcagagc tatgaagtct acgatctgct gaacgggctg atcgacgtgc agatgcggcg
      361 gatgaaggcc gcgatggcgg gagtgccgcg ggtggatgcg gtactcaagg ccgccgggct
      421 gcaggcgatc gaggtcgcgg cgaaggaggt tccggggagc ggcaatgccc ggaacgacga
      481 gggaggggaa gtcgagaact gacgatcgtc cgactgtccg actgtgttct ccgggggcgt
      541 ggtcaatcga tcctgaccac gccctcggcg attttcggga agcgtccgaa gaccgtcggg
      601 tcgtggccgg gcacgatgag cgctggcttc gacgcgagct tcagcatccg ctgctgcgcg
      661 gcgaggttgg agagggaatc cagcgtctgc gcgatgggac ggtgctcgtc gaggttgcgg
      721 tagagataga cgttgtcgga ggcgagcacg acgatgcccg agcgcgtggg cacggtgaga
      781 tattcggatg cgtacgtatg cttgccgccg aggtaggcgc gaatgccggg gaggacctcg
      841 gcgctgtcgc cgggaatctt cacgacgcgt cccgcggcgt tcaggcgaaa gagcatcgcc
      901 gcgtcgagcg aatcgatgac ggggtgcagc ggcgcgccgc cgggcccgac gtagtagtcg
      961 tattcgtcct gctgcaggcg aatgcgggcg ttgggaaaga gatcgacgcc gtcggcgtgg
     1021 tcccagtgga tgtgcgagag gatgatgtcc gaga
//
LOCUS       DASUCJ010000664         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_557362, whole genome shotgun sequence.
ACCESSION   DASUCJ010000664 DASUCJ010000000
VERSION     DASUCJ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557362"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFU90_07910"
     CDS             <1..1247
                     /locus_tag="VFU90_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479745.1"
                     /translation="GETFRALRNRNYRLFYIGQSISLCGTWMQTIAQAWLVLELTNSK
                     VALGTVTMLQFLPITIFVLFAGVIADRVPKRNFIVCTQTLAMAQAFVLAALVWSGHIQ
                     LWHLYVLASVLGLANAFEQPTRQAFVVEMVGREDLMNAVTLNSGLFNAARLIGPAIGG
                     FIIAVVGVKFAFLVNGVSFIPVIIGLLMMRMSELYGQAARSSGRVNPIAELREGIGYA
                     FRTPAALLIVILVAIIGTFGYNFTVMLPLIDKYVLHQGATGLGFMTAAVGLGALISAL
                     ALASRKSATKYTLFAGGAAFAVLLFAVALSQWYFVTLFFLLLLGVANTAFAATANTSL
                     QFATPDHLRGRVMALYMLLFAGSTPIGGYLTGWMADRLGVQTAVGIEAVLCMFGVAVG
                     LFYYFSHREKVIATSDIAGAAA"
BASE COUNT          206 a          483 c          446 g          264 t
ORIGIN      
        1 ccggcgagac ctttcgcgca ctccgcaacc gaaactaccg cctgttttac atcggccagt
       61 cgatctcgct ctgcggcacg tggatgcaga caatcgccca ggcgtggctc gtgctcgagc
      121 tgaccaactc gaaggtcgcg ctcggcaccg tcaccatgct ccagttcctg ccgatcacca
      181 tcttcgtgct cttcgccggc gtcatcgccg accgcgtccc gaagcgcaac ttcatcgtct
      241 gcacgcagac gctggcgatg gcgcaggcgt tcgtgctggc agcgctcgtc tggtccgggc
      301 acatccagct gtggcacctg tacgtcctcg cgtcggtgct cggcctcgcg aacgcgttcg
      361 agcagccgac gcggcaggca ttcgtggtcg aaatggtggg ccgcgaggac ctgatgaatg
      421 ccgtgacgct gaactccggc ctgttcaacg cggcgcgcct catcggcccc gcgatcggcg
      481 gcttcatcat cgccgtcgtc ggcgtgaagt tcgccttcct cgtcaacgga gtcagcttca
      541 tcccggtgat catcggcctg ttgatgatgc ggatgtcgga gctgtacggg caggcggcgc
      601 gatcgagcgg acgcgtgaac ccgatcgcgg agctacgcga aggcatcggc tacgccttcc
      661 gcacgcccgc cgcgctgctc atcgtcatcc tcgtcgccat catcgggacg ttcggctaca
      721 acttcacggt catgctgccg ctcatcgaca agtacgtgct gcaccagggc gcgacggggc
      781 tgggcttcat gacggcggcg gtcgggctgg gggcgctcat ctccgcactg gcgctcgcca
      841 gccgcaagtc cgcgacgaag tacacgctct tcgccggcgg cgccgccttc gcggtgctgc
      901 tcttcgcggt cgcgctgagc cagtggtact tcgtaacgct gttcttcctg ctgctgctcg
      961 gcgtcgcgaa caccgccttc gccgcgacag cgaacacgtc gctgcagttc gcgacgcccg
     1021 accacctgcg cggccgcgtg atggcgctct acatgctgct gttcgccggc tcgacgccga
     1081 tcggcggcta cctcaccggt tggatggctg accggctcgg cgtccagacc gccgtcggca
     1141 tcgaagccgt gctctgcatg ttcggggtcg cggtcgggct gttctactac ttctcgcacc
     1201 gggagaaggt catcgcgacg tcggacatcg ccggcgccgc cgcctaatgc ggctccgacg
     1261 cgtgacgttc agtcgtacgc gggtgtcatg ctgagcgaag cggcggtcat tctgagcgaa
     1321 gcgacgaatc tctccgaacc gaagagcggg gccgcgtcgt gcgcactaag agacccttcg
     1381 ctggcgctca gggtgacaa
//
LOCUS       DASUCJ010000665         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_694714, whole genome shotgun sequence.
ACCESSION   DASUCJ010000665 DASUCJ010000000
VERSION     DASUCJ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694714"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..331
                     /locus_tag="VFU90_07915"
     CDS             44..331
                     /locus_tag="VFU90_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479746.1"
                     /translation="MGKPHLTEFLLKLATDTPTLTTFQNCTADAQNQMMIDAKLTDDQ
                     IAAITSWDSREIVEAVCDELEANASSDTGYGGTTLTIILPMDNLQHPHLSE"
     gene            356..1153
                     /locus_tag="VFU90_07920"
     CDS             356..1153
                     /locus_tag="VFU90_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5479747.1"
                     /translation="MTAVGIGIRAPAQATLEATARIEAAQKVFSLVADPLAEYWLRTL
                     NQNTESLAGLYAIGKDRRDTYREMIERILAAVREPLHVCAVAYGHPGVAAYPLHESIR
                     LARAEGYRAEMLAGVSAEDCLFADLGVDPVAGGCVSYEATDFLLYRRAIDPAGNLVLW
                     QIGAIAEAGYKRERNGAWNPAGVAVLTEVLLETYPPEHEVVVYEAAQVPLAHPRSARV
                     ALARLTEAPMTAMSTLFVPPLAKSEIDATMMQRLGLSTESKTSPETG"
BASE COUNT          238 a          344 c          383 g          205 t
ORIGIN      
        1 cggtaagctt agcgctacgg gtcaggcacg gaggacaacc acgatgggaa aacctcacct
       61 taccgagttt ttgctcaagc tcgctaccga tacgccgacg cttacgacgt tccaaaactg
      121 cacggccgac gcacaaaacc agatgatgat cgacgccaag cttacggacg atcagattgc
      181 tgcgatcacg tcgtgggact cgcgggagat cgtcgaggcc gtgtgcgacg agttggaagc
      241 gaatgcatcg agcgacacgg gctacggtgg cacgacgctc accatcatct taccgatgga
      301 caacctgcag catccgcacc taagcgagtg acgggtgccg agcgcccgag gatcgttgac
      361 ggcggttgga atcggcatcc gtgcgcccgc gcaggcgacg ctcgaagcga cggcgcgaat
      421 cgaagcggcc cagaaagtct tctcgctggt cgcggatccg ctggcggaat actggcttag
      481 aacgctcaac caaaataccg aatcgctcgc cgggttgtac gcgatcggca aagaccggcg
      541 cgacacctat cgcgagatga tcgaacgcat actcgccgcc gtgcgcgagc cgctgcacgt
      601 ctgtgcggtg gcatacggtc atcccggcgt agcagcgtat ccgttacacg agtcgattcg
      661 tctcgcgcgg gcggaaggct atcgcgcgga gatgctggcc ggcgtttcgg cagaagattg
      721 tctatttgca gatctgggtg tcgatccggt ggcgggcggt tgcgtgagtt acgaagcgac
      781 tgattttctg ttataccggc gtgccatcga tccggccggc aacttggtgc tgtggcaaat
      841 cggcgcgatt gcggaagcgg gatataaacg cgaacgcaac ggcgcgtgga atccggccgg
      901 ggtcgcggtg ctgacggaag ttttgctcga aacgtatccg cccgagcacg aagtggtcgt
      961 gtacgaagcg gcgcaggtgc cgctggccca tccgcggagc gcgcgggttg cgctcgcgcg
     1021 gctgaccgaa gcgcccatga cggcaatgtc gacgctcttc gtaccgccgc tggcgaagtc
     1081 cgaaatcgac gcgacgatga tgcagcgtct cgggctgtcg accgaatcga agacctcccc
     1141 cgagaccggc tgaccgcagg catttcggat
//
LOCUS       DASUCJ010000666         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_802190, whole genome shotgun sequence.
ACCESSION   DASUCJ010000666 DASUCJ010000000
VERSION     DASUCJ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802190"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1320)
                     /locus_tag="VFU90_07925"
     CDS             complement(313..1320)
                     /locus_tag="VFU90_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU5479748.1"
                     /translation="MTAVMEAANEPKIVLLVRLLNAIDEGNHSFEALKDRISEGAKRP
                     STRSLRRYLAILADAGFPWYFDRPGNAYRFAGGYSLKRLDLSNGELFGLVALRSLGAS
                     IGGAIGSSIDEVTEKLVGTAGRSAKARVASHTPVAFRLSEIRLDEQGERAFALLSSAE
                     RAARSVQFAYNDKNGNRSVRTIDPYGFIINAGRVYCIGYDHGRRDKRTFAIDNVSDPD
                     VLAKTFVKPEGFRIEEYAAGSISGVLHGSETTAVRVRFDSRVAKAAIAARVVAERQVE
                     RRDDGSAEITYRVNDVDELVRWILGWGAQAEILDPPAARSRIATLTREVALKYNHATT
                     Q"
     gene            complement(1371..>2409)
                     /locus_tag="VFU90_07930"
     CDS             complement(1371..>2409)
                     /locus_tag="VFU90_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479749.1"
                     /translation="GTTTLTVQNVALPPFAPALLFYDDDPEHLTQDGVLFRGSVDAAQ
                     PAGLYYYHDDGSDPRRLIVALTGNSQDPTSVELIDSSAGPNIDVMQVGHSVSRNFLTI
                     EPHGEGAIVDLAGDDPVVLHDVSLAARQGAAGQIDLRVLSGGPVTVTVLAVSPGVDPR
                     SLLNAAVLPDDGHHRTRVFRIAGFGHDRLDYTAGGEDAKLVIGDRDPTPPSVDPAAPG
                     HDYGDYGVLHSLDVNLRNPGSAPVTAYLYFRPIAGIDRASFLVDGNLVELGCVREPVP
                     YQISSFELAPGQQSRATVQTMTDGGSFYPAEIGVTATPPQPSAPPITAPDGCFPRPAA
                     SPEPSPAPPHR"
BASE COUNT          377 a          801 c          823 g          408 t
ORIGIN      
        1 gtgaaacgtt gcgatgagcg acgctcccga tggtaccagt caaaccgccg atcgacgtca
       61 gtggggctct cccacccggg agccaaacag gtgaaatccg gtgagtgaat acgtcggaac
      121 ccgtcgaggc tacgcttcgg cgggtttcgt gattctcggg ccgcgtgcgc cggcgcgatt
      181 cgccgcgacg tccgcgctag tcaaaagggg ttacgcgcga agggcccgcg ccgaaccggc
      241 ccgctcgaac gccgcgcccg cacaacgcgg gcacgaagtg tgcgacggtg tagtaggacc
      301 gcggcttcgc agttattgcg tggtcgcgtg gttgtacttg agcgcgactt cgcgcgtgag
      361 ggtcgcgatc cgcgatcgtg ccgccggcgg atcgaggatc tcggcttggg cgccccaccc
      421 gagaatccag cgtaccagct cgtcgacgtc gttcacgcga tacgtgattt ccgccgaacc
      481 gtcgtcgcgg cgttcgacct ggcgctcggc gacgacgcgc gcggcgatcg ccgccttggc
      541 gacccgcgaa tcgaagcgca cgcgtaccgc ggtggtttcg ctgccgtgca gtacgccgct
      601 gatcgacccg gccgcgtatt cctcgatgcg gaatccctcc ggcttcacga acgtcttcgc
      661 gagcacgtcc ggatcgctga cgttgtcgat agcgaacgtt cgtttgtccc gacggccgtg
      721 atcgtagccg atgcagtata cgcgccccgc gttgatgatg aacccataag ggtcgatcgt
      781 acgaacgctg cggttgccgt tcttgtcgtt ataggcgaac tgcacgctgc gcgccgcgcg
      841 ttcggccgac gagagcagcg cgaacgcccg ctccccctgt tcgtcgagcc ggatctccga
      901 cagccggaag gccaccggcg tgtgcgatgc cactcgcgct ttcgcgctcc gtccggccgt
      961 accgaccagt ttctcggtga cttcgtcgat cgacgaaccg atcgccccgc cgatgctggc
     1021 gcccagcgac cgcagcgcca cgagcccgaa cagttccccg ttcgaaaggt cgagccgttt
     1081 gagactgtat cccccagcga agcgatacgc gttgcccggg cggtcgaaat accacgggaa
     1141 gccggcgtcg gcgagaatcg ccaggtagcg ccgcaggctg cgcgtgctcg ggcgcttagc
     1201 gccctcggaa atccggtcct tcagcgcttc gaaggagtga tttccctcgt cgatggcgtt
     1261 gagcagccga acgagcagga cgattttggg ctcgttggca gcttccatca cggcggtcat
     1321 tccggtgcgt cgccctccga ggacatttac gcaccgcggt tatgaaagtt tcaccggtgc
     1381 ggcggcgcgg gtgaaggttc cggcgaggcc gccggccgcg ggaaacagcc gtccggcgcg
     1441 gtgatcggcg gagccgacgg ctggggcggt gttgccgtca cgccgatctc ggccggatag
     1501 aaggagccgc cgtcggtcat cgtctgcacc gtcgcgcgcg actgctgccc gggcgccagc
     1561 tcgaacgagg atatctggta cggcaccggc tcgcgcacgc acccgagttc gacgaggttc
     1621 ccgtcgacca ggaaactcgc gcgatcgatg cccgcgatcg gccggaagta gagatacgcg
     1681 gtgaccggtg cgcttcccgg attgcgcaag ttgacgtcga gcgagtgcag cacgccgtag
     1741 tcgccgtagt cgtgtccggg ggctgccgga tcgacgctcg gcggcgtcgg gtcccggtcg
     1801 ccgatgacca gcttggcatc ctcgccgccg gccgtgtagt cgagcctgtc gtgcccgaat
     1861 cccgcgatcc ggaagacgcg ggtgcgatgg tgcccgtcgt cgggcagcac cgctgcgttc
     1921 agcagcgatc gcggatcgac gcccggcgag accgccagca ccgtcaccgt taccggaccg
     1981 ccggagagga cgcgcaggtc gatttgcccg gcggcgccct gacgcgcggc cagcgaaacg
     2041 tcgtgcagca ccaccggatc gtcgcccgcc agatcgacga tcgcaccttc gccgtgcggc
     2101 tcgatcgtga gaaagttccg gctgacggag tggccgactt gcatcacgtc gatgttcggc
     2161 ccggccgacg agtcgatcag ctccaccgag gtggggtctt gcgaattgcc ggttagcgcg
     2221 acgatcagcc gccgcgggtc gctgccgtcg tcgtggtagt agtacagccc ggccggctgc
     2281 gctgcgtcga cgctgccgcg gaagagcacg ccgtcttggg tgaggtgttc gggatcgtcg
     2341 tcgtagaaca gcagcgccgg cgcaaacggc gggagcgcca cgttctgcac cgtcagcgtc
     2401 gtcgtcccc
//
LOCUS       DASUCJ010000667         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1245388, whole genome shotgun sequence.
ACCESSION   DASUCJ010000667 DASUCJ010000000
VERSION     DASUCJ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245388"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1055)
                     /locus_tag="VFU90_07935"
     CDS             complement(<1..>1055)
                     /locus_tag="VFU90_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEU5479750.1"
                     /translation="SMSWRESYQPFARQREFHNSPAKYRLFGGAAGPGKTKALLWEAI
                     RQAHDVPGSDSLLLRRTYPELESSLLAYFRRDVPRSYYRKYNESKHIVTWKNGSTTRF
                     GHCRNENDVYAYQGAEYLFIAVDELTHFTLKQWQFLTSRNRCPIRGSRANMAGATNPG
                     NIGHAWVKALWVDHTPPPGFDRPELYDASDYDFVRAQITDNPIYANDAAYRRTLEVLP
                     EHLRRAFLDGDWNVFAGQYFDVFDIGRHTARPDDIRLEPWWPRWISIDWGFKHDSAVY
                     WHCAVPSNVHVAGPSQSQATNQQSPRIISYREFVQSGLSPRMLAQAIAERSGRERIAD
                     VFLSPDAFAHRTSESSI"
BASE COUNT          205 a          301 c          349 g          200 t
ORIGIN      
        1 gatcgaggac tccgatgtgc gatgcgcgaa tgcgtcgggc gaaaggaaaa catcggcgat
       61 gcgttcgcgg ccgctgcgct cggcgatggc ttgcgccaac atgcgcggtg ataatcccga
      121 ctgcacgaat tcgcgatacg aaatgattcg cggtgactgt tgattcgtgg cttgcgactg
      181 cgaagggcca gcaacatgca cattcgacgg aaccgcacaa tgccaataca cggcactgtc
      241 gtgcttaaag ccccagtcga tcgagatcca gcgcggccac cagggctcga gccgaatgtc
      301 gtccgggcgc gccgtgtggc gaccgatgtc gaacacatcg aaatattgcc cggcgaaaac
      361 gttccagtcg ccgtcgagaa acgcgcgtcg caaatgttcg ggcaggactt cgagagtgcg
      421 gcgataggcc gcatcgttgg cgtaaatcgg attgtcggtg atttgcgcgc gaacgaaatc
      481 gtagtcgctc gcgtcgtaca gctccggccg atcgaatccc ggcgggggcg tgtgatcgac
      541 ccacagcgct ttcacccacg cgtggccgat gttgccgggg tttgtggcgc cggccatgtt
      601 ggcgcggctg ccgcgaatcg ggcagcgatt gcgcgatgtg agaaattgcc actgcttcag
      661 cgtgaagtgc gtcagctcat cgacggcgat gaataaatac tccgcgccct gataggcgta
      721 gacgtcgttt tcgttgcggc aatggccgaa gcgcgtcgtc gagccgttct tccacgtgac
      781 gatgtgcttc gactcgttgt acttgcggta gtacgagcgc ggcacgtcgc gccgaaaata
      841 cgcgagcagc gacgattcga gctcgggata tgtgcggcgc agcagcagcg agtcggagcc
      901 gggcacatcg tgcgcctgtc ggatcgcctc ccagaggagc gctttggttt tgccgggacc
      961 ggcggcgccg ccgaagagcc ggtacttcgc cggtgaattg tgaaattcgc gttgccgcgc
     1021 gaacggctga tacgactcgc gccatgacat cgaag
//
LOCUS       DASUCJ010000668         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_7260, whole genome shotgun sequence.
ACCESSION   DASUCJ010000668 DASUCJ010000000
VERSION     DASUCJ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7260"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VFU90_07940"
     CDS             complement(<1..1687)
                     /locus_tag="VFU90_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HEU5479751.1"
                     /translation="MSQIAPYRAASPLAQPELRPPTASVADVHDVERLFAALLRRWRV
                     FAAVAGGFVALVAIATILTPKSYTTTVRLLAGRSDTTATNPDSNASLPVLNALVLQSG
                     AQSAETFAELAQQRNIASSVITSLNLPTTPRALLGGVSVRPVANTALLNLSVSWGSPD
                     GSAQIANAFANAFVDQEREFVRAEAVAAIGFLSKELPNAEARMRDTASRLADFQSKHG
                     YIDAATHEQDVVSRVASIDQRVDQLTVDEREAQALLNSASGQMASLSPTVDSAKEVDR
                     NPVSSDLSSKLADVETQLAQAEEKYTPAHPAVIALRQQKAALEAEIASQPSAVVGQTT
                     VAPNPIYQTLAQQATTYRARIQGDEAQIKELQNERRAYGSNVKTMPQEAVQFAAVSDE
                     AKRAANVYNALAQKYSEALVAKTTAISDIVIVQPASADAAVKRPSLLMNVSIALAVGI
                     LLGLATIYVLDAIERRSAERNFSALLGLPVIARIPEFEAKSQRMIPWIQSMTVEAFLH
                     LCVTLHHMNQRNTGTMAVLSARRGEGKSTVSYNLAKSLASLQPRVLLVDGDLRQ"
     gene            1854..3293
                     /locus_tag="VFU90_07945"
     CDS             1854..3293
                     /locus_tag="VFU90_07945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5479752.1"
                     /translation="MATRIVERPVLPPKSIARSAPPAYPARWTAILLLTDVVLFSVAS
                     WLGALVGFHHWNSPRIVGHLLIAELLYVALWVLVFDRLGLYRRTFALSMKDELYYTVA
                     ALGLGIVPQLVLFTIYPEISTSRIALIFGLLFSIVLVGSSRAILHRMRENQWFKSPRK
                     ISIVGTGEHVERVLESMELTADSETQLIVLDDVEQTIDQIDLSRDPELKRIEWFNHAR
                     TWGCDTIVMTEMVPPHLLAHILEIAASERIHLAFAPPKIVRYCYDVSLQTDGSQVLIV
                     PARLRACSPRAQLLKRSMDLVFGTFALMLFAPVMLLAALAIVVDSGWPILYAQERVGI
                     RGKTFRILKFRSMRPDAENAVGAVWATTDDPRKTRIGAFLRRFSIDELPQLFNVLRGD
                     MSLVGPRPERPVFVDLFRTTMPRYDQRHLVRPGITGWSHVHMKRNVDTSAAGERLAYD
                     LQYLENWSPYLDVAVLFQTLCEFLFHRAA"
     gene            3305..>4125
                     /locus_tag="VFU90_07950"
     CDS             3305..>4125
                     /locus_tag="VFU90_07950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5479753.1"
                     /translation="MRVLHILPSIRGYGAERLIVELLKQLPSREIDAALLTIYEPPAG
                     TQLPFQVLHAGRKNRRDRFFLWRLTQQIRRYKPDIVHTHTHVGKYWGRVAALLAGVPR
                     IVHTEHNPCDVRRTSVERVADRLLHRATSRVVTFFDDQAVLLSELDRLPTRKLVAIPN
                     GLMPSGLHEERLPARRRLELGGDQFAILLIGRMEYQKNHILALRSFAALPPQTRAKSV
                     LFFAGSGENEIMLRALTHGLKIAERVRFLGYRTDVPELLPGADLVLMTSWFEGMPL"
BASE COUNT          657 a         1338 c         1352 g          778 t
ORIGIN      
        1 gctgacggag atctccgtcg accagcagca cgcgcggctg cagcgacgcg aggctctttg
       61 ccaaattgta cgagacggtc gacttgcctt cgccgcggcg tgcgctgagc acggccatcg
      121 tcccggtgtt gcgctggttc atatgatgca gcgtgacgca caagtgcagg aacgcctcga
      181 cggtcatcga ctggatccac gggatcatgc gctggctctt ggcttcgaat tccggaatcc
      241 gcgcgataac cggaagcccg agcagggccg agaaattccg ttcggccgaa cggcgttcga
      301 tcgcatcgag gacgtagatc gtcgcgagcc cgagcaggat gccgaccgcg agcgcgatcg
      361 acacgttcat cagcaggctg ggacgtttga cggcggcatc ggcgctggcc ggctgcacga
      421 tcacgatgtc gctgattgcg gtggtctttg cgaccagcgc ttcgctgtat ttttgcgcga
      481 gcgcgttata cacgttggcc gcgcgcttgg cttcgtcgga aacggccgcg aactgcacgg
      541 cctcttgcgg catcgtcttt acgttggagc cgtaggctct acgctcgttt tgcagttcct
      601 taatctgcgc ttcgtcgccc tggatgcggg cgcggtacgt ggtggcctgc tgcgcgagcg
      661 tctggtaaat cgggttgggc gcgaccgtcg tctgcccgac caccgccgac ggctgcgaag
      721 cgatctcggc ttcgagggcg gctttctgct gccgcagcgc gatcacggcc ggatgggccg
      781 gggtgtattt ttcttcggcc tgggcgagct gcgtctcgac gtcggccagc ttcgagctga
      841 ggtcggagga aacggggttg cggtctacct ctttggcgct gtcgaccgtc ggcgaaaggc
      901 tcgccatttg cccgctcgca ctgttgagca aggcttgcgc ttcacgctcg tcgacggtca
      961 gctgatcgac ccgctggtcg atcgaggcga cgcgggagac gacgtcctgt tcgtgggtgg
     1021 cggcgtcgat gtatccgtgt ttggactgga agtcggccag ccgcgaggcc gtgtcgcgca
     1081 tccgggcttc cgcgttgggc agctccttcg aaagaaagcc gatcgcggcg acggcttcgg
     1141 cccgtacgaa ctcgcgctcc tgatcgacga acgcgttggc gaaggcgttg gcgatctgcg
     1201 ccgagccgtc gggactgccc cacgagacgc tcaggttgag cagcgccgtg ttggccaccg
     1261 gccgcacgct cacgcccccc aacagcgccc ggggagtcgt cggaagattg aggctcgtga
     1321 tgacgctgct cgcgatgttc ctctgctgtg cgagctcggc gaacgtctcg gccgattgcg
     1381 cgccgctctg caggaccagg gcgttgagca ccgggagcga ggcgttgctg tcggggttgg
     1441 tggcggtggt gtccgagcgg cccgccagga ggcgaaccgt cgtggtgtac gacttcggcg
     1501 tcaagatcgt ggcaatcgcg accagcgcca cgaagccgcc ggcgacggcc gcaaacaccc
     1561 gccagcgccg caggagcgcg gcgaacagcc gctcgacgtc gtggacgtcg gcgacggagg
     1621 cagtcggggg ccggagctcg ggttgggcca atggggacgc agcgcggtaa ggcgcaatct
     1681 gagacatcgc agctaaatcg tacgctgcgg ctaggtgccg gcataaggac gactctgctc
     1741 aaagcgtggg accttcgcag cagggacttg tgtcctttac ccccggagtc gcacgcggta
     1801 gtgctaacga ctctgcgaag cgcggcggca gcctgcgtat catttgagca tccatggcaa
     1861 cgcggatcgt cgaacgcccg gtactgccgc cgaagtcgat cgcccgcagc gcgcccccgg
     1921 catatccggc gcgctggact gccatattgc tccttaccga cgtggtgctc ttctcggtgg
     1981 cgtcgtggct gggagcgctg gtcggattcc atcattggaa ttcgccgcgg atcgtcgggc
     2041 atctgctgat cgcggaactg ctgtacgtcg ccctatgggt gctcgtcttc gatcgcctcg
     2101 gcctctatcg ccgtaccttt gcgctgtcga tgaaagacga gctgtactat acggtagcgg
     2161 cgctgggcct gggcatcgta ccccagctcg tgctgttcac gatctatccg gagatctcga
     2221 cgtcgcgaat cgcgctgatt ttcgggctgc tgttctcgat cgtgctggtc ggctcgtcgc
     2281 gcgccatcct ccaccggatg cgcgagaacc aatggttcaa gagcccccgc aaaatcagca
     2341 tcgtcggcac cggcgagcac gtggagcgcg tgctcgagag catggagctg accgccgatt
     2401 ccgagacgca gctgatcgtg ctcgacgacg tcgagcagac catcgatcaa atcgacttga
     2461 gccgggatcc cgaactgaaa cggatcgagt ggttcaacca cgcacggacc tggggctgcg
     2521 acacgatcgt catgaccgag atggtgccgc cgcacctgct cgcccacatc ctcgagatcg
     2581 cggcgtccga acgcattcac ctggcattcg ccccgccgaa gatcgtgcgc tactgctacg
     2641 acgtcagcct tcagaccgac ggcagccagg tgctgatcgt gccggcccgg ctgcgcgcgt
     2701 gctcgccgcg cgcccaactg ctcaaacgct cgatggacct cgtcttcgga acgttcgcgc
     2761 taatgctgtt cgcaccggtg atgctgctcg ccgcgctcgc gatcgtggtc gattcgggct
     2821 ggccgattct ctacgcgcaa gagcgggtcg gcatccgcgg caagaccttc cgcatcctca
     2881 aattccgctc gatgcggccg gacgccgaaa acgcggtcgg cgccgtctgg gcgacgaccg
     2941 acgatccgcg caagacgcgg atcggcgcgt tcctgcggcg cttcagcatc gacgagctgc
     3001 cgcagctgtt caacgtgctg cgcggcgaca tgtccctggt tggcccgcgg cccgagcggc
     3061 cggttttcgt cgatctcttc cggacgacga tgccgcgcta cgaccagcgc cacctcgtgc
     3121 ggccgggcat caccggctgg tcgcacgtcc acatgaagcg caacgtggac acctccgcgg
     3181 cgggcgaacg cctcgcctac gacttgcagt atctcgaaaa ctggtcgccc tatctcgacg
     3241 tagcggtgct gtttcagacg ctctgcgagt ttctgttcca ccgagcggct taggagccgt
     3301 ccgcgtgcgc gtgctgcaca tcctcccctc gattcgcggg tacggcgccg agcggctgat
     3361 cgtggaactg ctcaagcagc tgccgtcgcg agaaatcgac gccgccttgc tcacgatcta
     3421 cgagccgccg gccggcacgc aactgccgtt tcaagttctc cacgccggcc gcaagaatcg
     3481 gcgcgaccgc ttcttcctct ggcggctgac gcagcaaatc cggcgctaca aaccggacat
     3541 cgtgcacacc catacgcacg tagggaaata ctgggggcgc gtcgccgcgt tactggccgg
     3601 cgttccccgc atcgttcaca ccgagcacaa cccgtgcgac gtgcggcgca cttcggtaga
     3661 acgcgtggcc gatcggctgt tgcaccgcgc tacgtcccgg gtcgttacgt tcttcgacga
     3721 tcaggccgtc ctgctgagcg agctggatcg tttgccgacc cgcaaactgg tggcaattcc
     3781 gaacggcctg atgccgtcgg gtttgcacga ggaacgcctt ccggcgcgcc ggcgcctcga
     3841 gctgggcggc gatcaatttg cgatcctgtt gatcggacgg atggaatacc agaagaatca
     3901 catcctcgcg ctgcgctcgt tcgcggcgct gccgccccag acgcgagcca agtcggtgct
     3961 cttcttcgcc ggttcgggcg aaaacgaaat catgctgcgg gcgctcacgc acggcctaaa
     4021 gatcgccgag cgcgtccgct ttctcggtta ccggaccgac gttccggagc tcttgccggg
     4081 cgccgatctg gtgctcatga cgtcgtggtt cgagggcatg ccgct
//
LOCUS       DASUCJ010000669         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_802210, whole genome shotgun sequence.
ACCESSION   DASUCJ010000669 DASUCJ010000000
VERSION     DASUCJ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802210"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..889
                     /locus_tag="VFU90_07955"
     CDS             230..889
                     /locus_tag="VFU90_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479754.1"
                     /translation="MIPVYVGVAVLIVLIIAVFGGMKWWSAHELAVAQATPTPGPNTS
                     AKPVALADGGTIGARHFPIGDSKSGGTGQPVNGITCLTQEGEGLHIHAHVALFDHGKQ
                     MQIPRFIGFAPNPSLPGGGCLYWIHTHDASGIIHIEAPDLHPPGGGPYTLGLLFDIWG
                     QPLGTDGVAGFKGPVTAYVNGAKYDGDLRAIPMLAHQEITLEVGTPVVPPPLYTWPPN
                     D"
     gene            complement(1097..1666)
                     /locus_tag="VFU90_07960"
     CDS             complement(1097..1666)
                     /locus_tag="VFU90_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5479755.1"
                     /translation="MNQRQLGVLSIAVVVAIVASAITWYALAASHRLKSASQSPIVGK
                     AQVGQPAPEFAVATTGGPFDLKSTDRPVFLEVFATWCPHCQRETGVIDRLYQHFKGRV
                     DFVGVSGSDTGMDGTTAASQLDVLNWAQKFNVRYPIAYDASLDVANNYLQGGFPTLVV
                     IGKDKNVAYLNSGEVSYNDLAAALQKALK"
     gene            complement(1663..>2060)
                     /locus_tag="VFU90_07965"
     CDS             complement(1663..>2060)
                     /locus_tag="VFU90_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU5479756.1"
                     /translation="LLLSAGALKVGHAEELASAIAGFRLLPAFVVGPAALALPYFELL
                     LGGYLIVGLFVRSAAIVAGAQFFLYAVAVASAVMRHIPANCGCFGPNDTAVADWPHVA
                     FDLGLALLSGFIAYGAPGALALDRRFRSS"
BASE COUNT          384 a          684 c          652 g          340 t
ORIGIN      
        1 cggccggatg cgccgtcgcc gttacgccgc tggacgatgc ggtgaccgat tacgtcgcca
       61 actatttggt tccgggccgc ccgctcgatc cggccgacgc gtccgtcgcg ccggccctca
      121 aagcccccgc ttcataagag aggttcgctt gccacagcct tcccgcgccg aacggcgccg
      181 tcatgctcgc ggtaccggcg cttcggctcc gccgccgcat cgcgatccga tgattccggt
      241 gtacgtcgga gttgcggtgc tcatcgtgct gatcatcgcc gtcttcggcg ggatgaagtg
      301 gtggagcgct cacgagctcg cagtcgctca ggcgacgccg acacccggac ccaatacgag
      361 cgcgaaaccg gttgcgctcg ccgacggcgg cacgatcggc gcgcggcatt tccccatcgg
      421 cgattcgaaa tcgggcggca ccgggcaacc cgtcaacggc attacctgtc tcacgcagga
      481 gggcgaagga ctgcacatcc acgcgcacgt cgcgctcttc gaccacggca aacagatgca
      541 aattccgcgc tttatcgggt tcgcgccgaa tccgtcgctg cccggcggcg gctgcctgta
      601 ctggattcac acgcacgacg caagcgggat catccatatc gaagcgccgg atctgcatcc
      661 gccgggcggc gggccgtaca ccctcggcct gctgttcgac atttggggac aaccgttggg
      721 caccgacggc gtcgccggct tcaagggtcc ggttaccgct tacgtcaacg gtgcgaagta
      781 cgacggcgat ctgcgcgcga ttccgatgct cgcgcatcaa gagatcacgc tcgaagtcgg
      841 cacgccggtc gttccgcccc cgctctacac ctggcccccc aacgactaag gcgcgtctca
      901 cggctcttcg cctttatcgc tcggtagcgg gcgcgcgacg gcgggggtgc cgtgagcgag
      961 gcctccgact ttttcgcaaa agacagtcct tcgactgcgc tcgctaacgc gagctccgct
     1021 caggatgaca ccgttctaag ttgcgaaaaa gcggaggagt aggagcgcgg aaccgaagtg
     1081 ctaccgcgaa cgcgcgctac ttcagcgctt tttggagtgc ggcggcgagg tcgttgtacg
     1141 acacttcgcc gctattgagg taggcgacgt tcttgtcttt gccgatcacg accagcgtcg
     1201 ggaagccgcc ctgcaggtaa ttgttggcga cgtcgaggga ggcgtcgtac gcaatcgggt
     1261 accgcacgtt gaatttctgc gcccagttga gcacgtccag ttgcgatgcg gcggtcgtgc
     1321 cgtccatccc ggtgtcgctt cccgacacgc cgacgaaatc gacgcgaccc ttgaagtgct
     1381 ggtagagccg gtcgatgacc ccggtttcgc gttggcagtg cgggcaccac gtcgcaaaca
     1441 cttctaagaa cacgggccgg tcggtgctct tgagatcgaa cggtccgccc gtggttgcga
     1501 ccgcaaactc cggcgcgggc tgccccactt gtgcttttcc gacgatcggc gactgcgacg
     1561 cgctcttcag acggtgcgaa gcggcgagcg cgtaccaggt aatcgccgaa gcgacgatcg
     1621 cgaccacgac cgcaatcgaa agcacgccga gttgacgttg attcatgaag aacgaaatct
     1681 ccggtcgaga gcgagcgcgc cgggcgctcc gtaggcaatg aacccgctca gcagcgcgag
     1741 cccgagatcg aaggcaacgt gcggccagtc ggcaaccgcc gtatcgttgg ggccgaagca
     1801 gccgcagtta gccgggatgt gacgcatcac cgccgatgct accgcaactg cgtacaaaaa
     1861 gaactgagcg ccggcgacga tcgccgccga acgcacgaac agcccgacga tgagatagcc
     1921 gccgagcagc agctcgaagt aagggagcgc taacgcggcc ggccccacga cgaaggcggg
     1981 caacagccgg aagccggcaa tcgccgaagc gagctcctcg gcatgcccaa ccttcagcgc
     2041 gcccgccgaa aggagcaatc
//
LOCUS       DASUCJ010000670         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_282255, whole genome shotgun sequence.
ACCESSION   DASUCJ010000670 DASUCJ010000000
VERSION     DASUCJ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282255"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1340
                     /locus_tag="VFU90_07970"
     CDS             <1..>1340
                     /locus_tag="VFU90_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479757.1"
                     /translation="DQPYRDLAVNLLSTQSYPSTAQYPPYDDIAWTLGALYGVQVRAV
                     NDTSVFHWTGLERLEDTVAYSGAVRGAGPVYLLAYRAQSEVLPALFQLRERDRAATAA
                     VAEHAFVVGQDSFPAGSVIIEHAPAGAAKEIAERWGLPLVSADRAPDVQRHAVDLPRI
                     AVYHSWYDTQDAGWSRYTFDQLGIPYTSIDKDDLRRGALRKRFDVILVPNMNGSLDRM
                     INGVDRDFGPLPFETTSATPNLGTPFSSPDITGGPGFAGLEALRAFVDDGGELITLET
                     STRLAAESGIAAELSPHPTRTLFHPGSVVRVRMRHASPILYGFPDTTTVFRGNAPLYE
                     VPARDSAMLILQYGTKLPEAADTGAIMGEPNKAAPPRDSTAKSKKHGAEEPYVVSGMV
                     RGEDEIIGQGAIFDVPVRKGHVVAFTFDPLHRYLNHHEFPMVWNAIINWNVAPP"
BASE COUNT          225 a          456 c          448 g          211 t
ORIGIN      
        1 tcgaccagcc gtatcgcgac ctcgccgtca acctgctcag cacgcagagc tacccgagca
       61 cggcgcagta tccgccctac gacgacatcg cgtggacgtt aggcgcgctc tacggggtgc
      121 aggtgcgcgc ggtgaacgac acctcggtgt ttcactggac cggtctcgag cgcctcgagg
      181 acacggtcgc gtacagcggc gcggtgcggg gcgccggccc cgtctacctg ctcgcctacc
      241 gtgcgcagag cgaagtgctg cccgcgctct tccagctgcg tgaacgcgac cgcgcggcaa
      301 ccgcggcggt ggcggagcac gcgttcgtcg ttgggcagga cagcttcccg gcgggatcgg
      361 tgatcatcga gcacgcgccg gccggcgcgg cgaaggagat cgccgaacga tggggcttgc
      421 cgctggtgag cgcggaccgt gcgccggatg tccaacggca cgccgtcgac ctgccgcgca
      481 tcgccgtcta ccattcgtgg tacgacaccc aggacgccgg ctggtcgcgc tacacgttcg
      541 atcagttagg cattccgtac acgtcgatcg acaaggacga tctgcgccgc ggagcgctgc
      601 gcaaacggtt cgacgtcatc ctggtgccca acatgaacgg gtcgctcgac cgaatgatca
      661 acggcgtcga tcgcgacttc gggccgctgc cgttcgagac gacgtccgcg acgccgaact
      721 tggggacgcc gttctcgagc cccgacatca ccggcggccc cgggtttgcc ggtctcgagg
      781 ccctgcgcgc gttcgtcgac gatgggggcg agctgatcac gctcgagacg tcgacgcgcc
      841 tcgcggcgga gtcgggcatc gccgccgagc tctcgccgca tccgacgcgg acgttgttcc
      901 atccgggatc cgtcgtgcgc gtgcgcatgc gacacgcatc gccgatcctc tacgggttcc
      961 ccgacaccac caccgtgttt cgcgggaatg cgccgctcta cgaagtgccg gcgcgcgaca
     1021 gcgcgatgct gattctgcag tacggcacca agctgccgga agcggcggat accggcgcga
     1081 tcatgggcga gcccaacaag gccgcaccgc cgcgcgactc gacggcgaaa tcgaagaagc
     1141 acggagcgga agagccgtac gtcgtgtccg gcatggtgcg cggcgaggat gagatcatcg
     1201 ggcaaggcgc catcttcgat gtgccggtcc gcaaaggaca cgtggttgcg ttcacgttcg
     1261 atccgctgca tcgctatctc aaccatcacg aattcccgat ggtctggaac gcgatcatca
     1321 actggaacgt ggcgccgcct
//
LOCUS       DASUCJ010000671         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1321683, whole genome shotgun sequence.
ACCESSION   DASUCJ010000671 DASUCJ010000000
VERSION     DASUCJ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321683"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFU90_07975"
     CDS             complement(<1..483)
                     /locus_tag="VFU90_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479758.1"
                     /translation="MTWVYAMPAWLFSVAVFGVSTVLAGGGLLLARRIVKQSGEVTHN
                     DVAGPIIGTVGTILAVVLSFLLVTVWQEYDGAAGTVSQEASAVADLFHLAAHFPQPVA
                     GELRSTLRTYVDVVVYDEWPAMQSGGTSPQARAGALETFAIVARFQPRTAADQALQQQ
                     A"
     gene            616..689
                     /locus_tag="VFU90_07980"
     tRNA            616..689
                     /locus_tag="VFU90_07980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Gly,seq:ccc)
     gene            716..790
                     /locus_tag="VFU90_07985"
     tRNA            716..790
                     /locus_tag="VFU90_07985"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Cys,seq:gca)
     gene            801..1754
                     /locus_tag="VFU90_07990"
     CDS             801..1754
                     /locus_tag="VFU90_07990"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5479759.1"
                     /translation="MRVAVVGAGAIGGFLAGALARAGIDVAVVARGEHLAAIRRDGLR
                     IDGDLGEFRVRPRAAADLGELGEFEFLLLTFKAHQWPVLFPQLAPYARSATTLVTFQN
                     GLPFWYVREPPLRSVDPDGIIGATFPDDQVLGGVVHVSGHLVEPGHVRQSGGLRYVAG
                     DPRGGRSARLEALVAAMRGAGLAAEADANVRSTVWLKLVNNVGLNTVSALRRMTIRPL
                     LGDMVARAEVRELMSEALRVGQAMGVVDEVDIDARIEYAARLDDVKTSTLQDLERGRA
                     LELEPIAGAVIELAERYGVDVPAIRASYATLLDAQERARHG"
     gene            1747..>2806
                     /locus_tag="VFU90_07995"
     CDS             1747..>2806
                     /locus_tag="VFU90_07995"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5479760.1"
                     /translation="MDEPVARLSGVGPKTAPLFAALGIDTAHALLNYFPFRYEDLRFA
                     TPASRLGESGGEENAAGRIVNVKERRVRHLEIVDVTLHDDSGGTFFAKWIGQRRYVYG
                     RFRPGMRIFVRGRVERTLAGPVVNVTQYGALADDETYRGELVPVYRASKDLASRKIAV
                     VVKKNFATLLETAGEDPLPPEIVEQRRYLALRDAYRAVHAPSQPDDAQRARERFVFTE
                     FLALATAAALRRARRSRDHGARALTVPAGLLDEFAASLPYALTAAQRAAIAEIWDDMS
                     RDVPMNRLLQGDVGSGKTLVAAAAILLAARSNVQSALMAPTEILAAQHAAKLAPLLLP
                     FGITVEAVFGSQSAKARAS"
BASE COUNT          434 a          913 c         1006 g          453 t
ORIGIN      
        1 ggcttgctgc tgcagcgctt gatcggcggc cgtgcgcggc tgaaaacgcg cgacgatggc
       61 gaacgtctcg agcgcgccgg cgcgcgcttg cgggcttgtg ccccccgact gcatcgccgg
      121 ccattcgtcg tagaccacga cgtcgacata cgtgcgcagc gtgctgcgca gctcgcctgc
      181 aaccggttgc ggaaagtgcg cggccaaatg aaagaggtcc gcgaccgcac tcgcctcttg
      241 cgagaccgtc cccgcggcgc cgtcatactc ttgccacacg gtgacgagca gaaacgagag
      301 cacgaccgcc aagatcgttc cgaccgttcc gatgatcggc ccggcgacgt cgttatgggt
      361 cacctcgccg ctctgtttga cgatgcggcg tgcgagcagc aggccgccgc cggcgagtac
      421 cgtcgagacg ccgaacaccg cgaccgagaa aagccaagcc ggcatcgcgt agacccaggt
      481 cacgccgttg gccttcgcgc gggagggggt gcctccccgt gcgcatggtt gcgccgagcc
      541 gcaacgctta cgccggccgc gcggtttgta ggacggggtc cggcgttgct cgaaccggtg
      601 ggctcgccgc ggtgcgcggg tatgatgtaa tggcagcctg cgaccttccc aaggttgatg
      661 cgtgggttcg attcccacta cccgctccac cggacggcgc ggaggcgcgt gacaaggcga
      721 cgtagccaag tggtaaggca gggctctgca aaagccccat tcggcagttc aaatctgccc
      781 gtcgcctcca acgttctttc atgcgcgtag cggtggtcgg tgccggcgcg atcggcggct
      841 ttctggccgg cgcgctggcg cgcgccggaa tcgacgtcgc ggtcgtcgcg cgcggcgagc
      901 atttggcggc aatccgtcgc gacgggctaa gaatcgacgg cgatctgggc gagttccgcg
      961 tccgtccgcg cgccgccgcc gatttgggcg aactgggcga gttcgagttc ttgctgctga
     1021 cgttcaaggc gcaccaatgg ccggtgctgt ttccgcagct ggcgccgtat gcgcgctccg
     1081 cgaccacgct ggtgaccttc cagaacgggc tgccgttttg gtacgtccgc gaaccgccgc
     1141 tgcgcagcgt cgacccggac ggaataatcg gggcaacctt tccggacgat caggtcctcg
     1201 gcggggtcgt gcacgtttcc ggacacctcg tcgagcccgg gcacgtgcgt cagagcggcg
     1261 gcctgcgcta cgtcgccggc gatccgcgcg gcggccggtc cgcgcggctg gaggcgctcg
     1321 tggcggcgat gcgcggcgcg gggctggcag ccgaggccga tgcgaacgtt cgctcgacgg
     1381 tgtggctgaa gctggtcaac aacgtcgggc tcaataccgt cagcgcgctg cgccggatga
     1441 cgatccggcc gctgctgggc gatatggttg cgcgcgccga agtgcgcgaa ctgatgagcg
     1501 aggcgctgcg cgtcgggcag gcgatgggcg tggtcgacga ggtcgatatc gacgcgcgaa
     1561 tcgaatatgc cgcgcgtttg gacgacgtca agacgtcgac gctgcaggat ctcgaacgcg
     1621 gacgtgcgct cgagctggag ccgatcgccg gcgcggtcat cgagctggcc gagcgctacg
     1681 gcgtggacgt tcccgcaatc cgggcgagct acgcgacgct gctcgatgcg caggagcgcg
     1741 cgcgccatgg atgaaccggt cgcgcggctg agcggcgttg ggccgaagac ggcgccgctg
     1801 ttcgccgcgc tcggcatcga taccgcgcac gcgctgctga actactttcc atttcgctac
     1861 gaggatctgc gctttgcgac gccggcgtcg cggctgggcg aatcgggtgg tgaagaaaac
     1921 gccgccggcc ggatcgtcaa cgtgaaagag cggcgcgtac gccatttgga gatcgtcgac
     1981 gtgaccctgc acgacgactc cggcggcacg tttttcgcaa aatggatcgg acagcgccgg
     2041 tacgtctacg gccgcttccg gcccgggatg cgaatcttcg tgcgcggccg ggtcgagcgc
     2101 acgctggcgg gtccggtggt gaacgtcact cagtatggag cgctggccga cgacgaaacc
     2161 taccgcggcg agctggttcc ggtgtaccgg gcgagcaagg atctcgccag ccgtaaaatc
     2221 gccgtggtcg tgaagaaaaa tttcgcgacg ctgctggaga cggccggcga agatccgctc
     2281 ccgccggaga tcgtcgagca gcgccgctac ctcgccctgc gcgacgcata ccgggcggtg
     2341 cacgctccgt cgcaacccga cgacgcgcag cgcgcgcgcg agcgattcgt cttcacggag
     2401 ttcctggcgc tggcgaccgc cgccgcgctg cggcgcgcgc ggcgcagccg ggatcacggt
     2461 gcgcgggcgc tgacggtccc ggccgggctg ctcgatgagt ttgcggcgtc gctgccgtat
     2521 gcgttgacgg ccgcgcagcg ggcggcgatc gccgaaatct gggacgacat gtcccgcgac
     2581 gtgccgatga accgcctgct gcaaggcgac gtcggcagcg gtaagacgct ggtggcggcg
     2641 gcggcaattc tccttgcggc acgcagcaac gtgcagtcag ccctgatggc gcccactgag
     2701 attctcgccg cgcagcatgc ggcgaagctt gcgccgcttt tgctgccgtt cggcataacg
     2761 gtggaagccg tattcggcag tcaaagtgcc aaagcgcgcg cgtcag
//
LOCUS       DASUCJ010000672         1461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_99092, whole genome shotgun sequence.
ACCESSION   DASUCJ010000672 DASUCJ010000000
VERSION     DASUCJ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99092"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1461
                     /locus_tag="VFU90_08000"
     CDS             <1..>1461
                     /locus_tag="VFU90_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5479761.1"
                     /translation="SPMRAKVVFYALVATAAFLVWRLYDVQVVHGALYAERALAQRSD
                     TVEVFARRGSILDRNGNVLVRSLPSESVYAVPRDLADPGAAIAKLQKIFGKLDPSTVA
                     ALHDKTLWFAWIARKVPHDVAERVRALNVGGIALKEEQTGLRVDPAGKLAATVLGFVG
                     TDENGLDGIEYSQDRLLRGRSGAVVLETDEFGHPIPFGHERAVRPAEPGSSIELTIDS
                     YLQFVTERALAKQVGAFHALDGTAIVMDPWTGEVLAMANVPTFDPNRFWKYTGDQRRD
                     RAVMDAYEPGSTYKLLTAAAALDSRKVSLTSRFPAHDALVVGGRTIHNAEDGFMAGTG
                     SSETLAQIVEYSHNVGAAEVGMYIGGKTFYAMEQKAGFGTSTGIGLPGENPGIVPAPK
                     DWSASSLATMSFGQGVSVTPLAMVRYYSAIANGGLLMEPHVIRAVYDDDGNLVRKIAP
                     RVVRRVFSKRTAAELRGFLRLVVLHGTGNPTAQIPG"
BASE COUNT          257 a          478 c          475 g          251 t
ORIGIN      
        1 ctctccgatg cgggccaaag tcgtttttta cgcgctggtg gcgacggcag cgtttctcgt
       61 atggcgactg tacgacgtgc aggtcgtgca cggcgcgctc tacgcggagc gcgcgctcgc
      121 gcagcgatcc gatacggtcg aagtcttcgc gcgccgtgga agcattctcg accgtaacgg
      181 caacgtgttg gtgcgctcgc tgccctccga aagcgtctac gcggtgccgc gcgatctggc
      241 cgatcccggc gccgcgattg cgaagctgca gaagatcttc ggcaagctcg acccgtcgac
      301 ggtcgccgcg ctgcacgaca agaccttgtg gtttgcgtgg atcgcacgga aggttccaca
      361 cgacgtcgcc gagcgcgttc gcgccctgaa cgtcggcggc atcgcgctca aggaagaaca
      421 aacgggtttg cgcgtcgatc cggcgggcaa gctggcggcg accgttctcg gcttcgtcgg
      481 aaccgacgaa aacgggctcg acgggatcga gtactcgcaa gaccgcctgt tgcgcggccg
      541 ctcgggcgcg gtcgtgctcg aaaccgacga gttcgggcat cccattccgt tcggtcacga
      601 acgagccgtc cgtccggccg aaccgggctc gagcatcgag ctgacgatcg actcgtacct
      661 gcagttcgtc accgaacgcg cgctcgccaa gcaggtcggc gcgtttcacg ccctcgacgg
      721 aaccgcgatc gtgatggatc cgtggaccgg cgaagtgctt gctatggcaa acgttccaac
      781 cttcgatccc aaccgctttt ggaagtacac cggcgatcag cgccgcgatc gcgcggtcat
      841 ggacgcctac gagccgggct ccacctacaa gctcttgacc gccgccgcgg cactcgactc
      901 gcgcaaggtc agcttgacgt cgcgcttccc cgcccacgac gcgctggtgg ttggcgggcg
      961 aaccatccac aacgcggaag atggtttcat ggccgggacc gggagcagcg aaacgctcgc
     1021 gcagatcgtc gagtactcgc acaacgtcgg ggcggccgaa gtcggcatgt acatcggggg
     1081 aaaaacgttc tacgcaatgg agcagaaggc gggtttcggg acgtctaccg gaatcggtct
     1141 gcccggcgaa aaccccggca tcgtgccggc tcccaaggat tggagcgctt cgtcgctggc
     1201 gacgatgtcg ttcgggcaag gcgtttcggt aacgccgctc gcgatggtcc gctactacag
     1261 cgcgatcgcc aacggaggtt tgctgatgga gccgcacgtc atccgcgcgg tctacgacga
     1321 cgacggcaac ctggttcgca agatcgcgcc gcgggtcgtg cgccgcgtct tctccaaacg
     1381 caccgccgcc gaactgcgcg gcttcttacg attggtcgtg ctgcacggaa ccggcaaccc
     1441 cacggcgcag attcccggat a
//
LOCUS       DASUCJ010000673         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1031310, whole genome shotgun sequence.
ACCESSION   DASUCJ010000673 DASUCJ010000000
VERSION     DASUCJ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031310"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            672..884
                     /locus_tag="VFU90_08005"
     CDS             672..884
                     /locus_tag="VFU90_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479762.1"
                     /translation="MMMLVSFLVDRRRMPHLGADRHALDAVIRAPNRQCERDTIADAQ
                     HSRRSDKLDPYGTRLQMNHRSGWKCN"
     gene            complement(1173..1331)
                     /locus_tag="VFU90_08010"
     CDS             complement(1173..1331)
                     /locus_tag="VFU90_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479763.1"
                     /translation="MSEQKPITKVPDGKTPFYTTRQMAPNAKGFVGYETVWKPFQKEV
                     AYKTPKTP"
     gene            complement(1372..>1657)
                     /locus_tag="VFU90_08015"
     CDS             complement(1372..>1657)
                     /locus_tag="VFU90_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479764.1"
                     /translation="LVFTDRLGNVPDEYRQVGTSILMENDKGQTRSFIVTRIDNDTLT
                     IDGNNPLCGREVVFTLEILTVREATGDEMKAGGVIGAAPDVDPSLMRPVS"
BASE COUNT          296 a          525 c          554 g          282 t
ORIGIN      
        1 aatgtgcaga tcgtcccgcc tggtcgaggt ttgccgaaac gcgagaatgc gcgccggccg
       61 aggtgcaatc gcaggcggac gtggaagcat cagcgaccgc cgcgacccgt cggacgaact
      121 gcgagcatcg aatctccgac gccgcaacgg cgccggatga tcgcatctac gcgagcgcgg
      181 gaaggagcgg cggcgcccgc gcgtcaggaa gggatcgcct cggcgggcgc gccgacgcgc
      241 gaacgactgg ctcgagcgca ggcggcggtg tgaacgaccg cagcgtggca atgctggccg
      301 cggaaaccag ggctctcgcc ccggagagcg cgcagagcgc gcacccgccg caactcgcgg
      361 ggccggactg gtgattcgag gtgccgccgg acgtcggatg cgcgcggcac aaaccgtcgc
      421 ccgaatgatc gacgaccgcc agtgccggga tgggaagaag gagctgagcg agcagcgcga
      481 aaaacccgac cgtcctcctc gttcgtgcac gcatggtgct gcgatgctag cacagcggca
      541 cgccgcaaac gcagtagccg ccgcagcgga tgggcatcgg ttgctgagcg gacccacgag
      601 tgccgaggat cccgtggcga atccgctgcg tgagctgatt tagcgacttt gagccgcgat
      661 atttaacgtg cgtgatgatg ctcgtgagct tcctcgtcga ccgccgacgc atgccgcacc
      721 tcggcgctga tcgacacgcg ctcgatgctg tgatccgcgc gccgaatcgt cagtgtgagc
      781 gggacacaat cgccgacgcg cagcactcgc gtcgttccga caagctcgat ccgtacggca
      841 cccggcttca gatgaaccac cgttccgggt ggaagtgcaa ttgagggcga cggtccgctg
      901 gtaacaccga ggcagcgagc gccggtgcgg ccgccaccat gatcaacacc gcgacgccga
      961 atctcatcgg tcgtcatgcc tgacgcttca cggggagcgg catcggaatc tatcgtcgaa
     1021 gactcgacca gttatcgtcc gcgaacaccg gcggcgtggt atccattccg ccccggcatc
     1081 actccttcgg tctgatttgc agatatcgga cgcggccccg ttgtcgacga gatcgccccc
     1141 gcgggtccgc gggtccggta cgcagtgcgg gtctacggcg tcttcggtgt cttgtacgcg
     1201 acctccttct ggaacggctt ccagacggtt tcgtagccga cgaacccctt ggcgttcggc
     1261 gccatctgac gcgtcgtata gaagggcgtc ttgccgtcgg gaaccttggt gattggcttt
     1321 tgctcgctca tcggtcacct ccatgggtgc gaagtcagga aatgtggctc atcaggaaac
     1381 ggggcgcatc agcgacgggt caacgtcggg tgcagcgccg atcacgccgc cggctttcat
     1441 ctcgtcgccg gtggcttcgc gcaccgtcag gatttcgagg gtaaagacga cctcccggcc
     1501 gcagagcgga ttgttgccgt cgatcgtcaa tgtatcgtta tcgatgcgcg tcacgatgaa
     1561 gctgcgggtc tggcccttgt cgttctccat caggatggag gtgccgacct gccgatactc
     1621 gtcgggcacg ttgccgaggc gatcggtaaa gaccagc
//
LOCUS       DASUCJ010000674         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1260532, whole genome shotgun sequence.
ACCESSION   DASUCJ010000674 DASUCJ010000000
VERSION     DASUCJ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260532"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..124)
                     /locus_tag="VFU90_08020"
     tRNA            complement(52..124)
                     /locus_tag="VFU90_08020"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:His,seq:gtg)
     gene            518..1048
                     /locus_tag="VFU90_08025"
     CDS             518..1048
                     /locus_tag="VFU90_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479765.1"
                     /translation="MTGVLAVAALFVVAIPATGASAAKLLKLSSEKVMVPNGSPAHVG
                     LAIAGCVIVSEGTLTVNDAAKDKLVSASDMFAECETAGESASGVITEAQMGGTGKTSM
                     KGKISITKPGPCVYVYSKWKGVFAVPGFSSMEFSTTGKINKKVSAKEGCAATDTEAVA
                     AEAADEALSPFEATLG"
     gene            complement(1155..>2133)
                     /locus_tag="VFU90_08030"
     CDS             complement(1155..>2133)
                     /locus_tag="VFU90_08030"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5479766.1"
                     /translation="TAYAAPIAPGAVTDAPPSASWGTPTTGEALIAEQPDELLALATD
                     DFGVTSVHIIAGEEDLGTMSSAPYETTWTPPASLIGQSVTVMATVTDESGQTSVATEQ
                     VPVIAKPLAVEEKLGEEPHVEPKTEAVTPLVVSTPGAGPAPVIAIPATFARSALHSLD
                     LTPSITGSAAIVGVEYLLDNRTICKLTAAPFACHYLPTGADVGLHTLKIIATDASGET
                     TQVSESIRFARFAVHGIALHVKRSGSTLKLTGAIELPARVTHAEGCAKETVHVTFARG
                     RHKSSGVGTVTPRCTFSVSLAHAGGTGKLTASLSGNSVLAPLSRAAKVR"
BASE COUNT          351 a          685 c          740 g          357 t
ORIGIN      
        1 cccgacagcg accatcagac aaagacacag ctatttgcag cggctatcag gggtgagcga
       61 ccggattcga accggcgacc gcctggacca caaccaggag ctctaccaac tgagctacgc
      121 ccaccacggg aggagacgga gtctagcgtc ggagcctcgg ctccgccggc ggcccgcgcc
      181 tcgctcccgc gggtcgacga cggcgacgcg gcccgacctc tgtcgctcgc ttgggacttg
      241 caggctcgcg agagcgctca gatcaagccc tgagcttacg gtttcacctg atgcggagaa
      301 acccgtatca gttagaaaca gactttgcgt gagccttcgc catctggaaa gctcacgttc
      361 gttacatcga cgtggggatg atgtaccggc atttcacctg ccgggggctc gtagcgctct
      421 gttgtcttgt caaggacgac gcgctggtcc ctgcgtctaa acctggaact ggacaagagg
      481 gatgagggag gaataaatga gaaggatcaa actatacgtg accggcgtgc ttgccgtcgc
      541 ggctctcttc gtggtggcga tcccggcgac gggcgcctcg gccgcgaagc tgctgaagct
      601 gagcagcgaa aaagtgatgg tgccgaacgg ctcgccggcg cacgtcggcc ttgcgatcgc
      661 gggatgcgtc atcgtcagcg aaggcactct caccgtcaac gacgcggcga aggacaagct
      721 ggtctccgcc agcgacatgt tcgccgaatg cgaaaccgca ggcgagtcgg cgtctggcgt
      781 gatcaccgaa gcgcagatgg gcggcaccgg caagacgtcg atgaagggca agatctcgat
      841 caccaaaccg ggcccgtgcg tctacgtcta cagcaagtgg aagggcgtgt tcgcagtgcc
      901 tggcttctcc tccatggaat tcagcacgac gggcaagatc aacaagaaag taagcgccaa
      961 agaaggatgc gcggcgaccg acaccgaggc cgttgcggct gaggccgcgg acgaagcgct
     1021 gtcgccgttc gaagccacgc tcggctagct gaagctgggt gttcgcaagc tgggcccggg
     1081 cgctctgacg cgcgcccggg cccgcttcgc gcgccgagca tcgcgagcta tgcgtatgcg
     1141 atctcggccg gcgtctagcg gactttcgct gcgcggctga gcggcgcgag cacggagttc
     1201 cccgacaggc tcgccgtcag cttgccggtg ccgcccgcgt gcgcgaggct cacgctgaac
     1261 gtgcagcgcg gcgtgaccgt gccgacgccg gaggacttgt gcctgccgcg cgcgaacgtc
     1321 acgtggacgg tctccttcgc gcagccctcg gcgtgcgtga cgcgcgcggg gagctcgatc
     1381 gcgccggtca gcttcagcgt cgagccgctg cgcttgacgt gcagcgcgat gccgtggacg
     1441 gcgaagcgcg cgaagcggat cgactcgctg acctgcgtcg tctcgccgct tgcgtcggtc
     1501 gcgatgatct tcagcgtgtg cagcccgacg tcggcgccgg tcggcaggta gtgacaggcg
     1561 aacggcgccg ccgtcagctt gcagatcgtg cggttgtcga gcaggtactc gacgccgacg
     1621 atcgccgcgg agccggtgat gctcggcgtc aggtcgaggc tgtgcagcgc cgatctggcg
     1681 aacgtcgccg ggatcgcgat gaccggggcc gggccggcgc ccggcgtcga gacgacgagc
     1741 ggcgtgaccg cttccgtttt cggttcgacg tgcggttctt cgcccagttt ttcttcgacc
     1801 gcgagcggtt tggcgatgac cggcacctgc tcggtcgcga cgctcgtctg ccccgactcg
     1861 tcggtcaccg tcgccattac ggtgaccgac tggccgatca gcgacgccgg cggcgtccac
     1921 gtcgtctcat acggcgcgct ggacatcgtg ccgaggtctt cttcgccggc gatgatgtgc
     1981 accgaggtca cgccgaagtc gtcggtcgcg agcgcgagca gctcgtcggg ctgttcggcg
     2041 atcagcgctt cgccggtcgt cggcgttccc catgacgcgc tcggcggcgc atcggtgacc
     2101 gcgccgggcg cgatcggcgc ggcgtacgcg gtt
//
LOCUS       DASUCJ010000675         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1000639, whole genome shotgun sequence.
ACCESSION   DASUCJ010000675 DASUCJ010000000
VERSION     DASUCJ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000639"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFU90_08035"
     CDS             complement(<1..841)
                     /locus_tag="VFU90_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479767.1"
                     /translation="MNKFCFVVHPLSLDDVARYEPGAKGKGDAIMRKIMEWMPPWAAV
                     HVTGVRAPDGREVEGWFVSASLLPEQMVELPREEVYGRILAAIQIGADLGAQVAGLGA
                     FTAVVGDGGITINERSPIPVTTGNSLTIGAGVQSFFRAAQEMNIDVAESTAVVIGATG
                     SIGSACLQLIAPKVNHVVMVARNETRLRKFHERIAGELPCESSYTTDVGAAVRRAQLV
                     LTATSSTQEIVEPEDLRTGAVVCELSLPHDVSRRVARERPDVLVTEGGNMSVPGNPRF
                     ERVR"
     gene            complement(1074..1535)
                     /locus_tag="VFU90_08040"
     CDS             complement(1074..1535)
                     /locus_tag="VFU90_08040"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5479768.1"
                     /translation="MPYVESAIAIAAPAKIVYELAKDQERFPEFMPDVETVTVLERHP
                     DRVISRWKTLVEEAPIEWTEEDRFDDEALRIDYKLLEGDLDKFEGSWTFAERDGTTHV
                     RLGVDYDFGVPTLAELIGPTLQKKVVENSEMMLAALKRQAEAAATGEGSQT"
     gene            complement(1552..2823)
                     /locus_tag="VFU90_08045"
     CDS             complement(1552..2823)
                     /locus_tag="VFU90_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5479769.1"
                     /translation="MSLRPPSPPSADIAAEAFENYRQFVNPPLARVMKLSGSPAEVRA
                     RGCTIWDQNGKAYLDFAGGYGVFTLGHSHPRVLHAVREQMELMSLSGKTMFNPVLGRA
                     ARRLAEIAPGDLRISFWCNSGTEAIEGAIKLARAATGRAKIVSTLGAFHGKTMGALSV
                     SGRESFQTPFRPLLEGFAQIPYGETEQLETVLSDAAAFVVEPVQGEGGVNVPPDGYLR
                     AVRTACDRSGALLVADEVQTGLGRCGYRFACEREGVVPDAMTLAKGLSGGVIPVGAFI
                     ARPDAWERAFGKAPLLHTSTFGGGELACTAAIAAIDVLEDEGLAENARVRGEQLLEGA
                     RAIAAEFPAVVREARGLGLMVGVELASEGYGGWIIAEMLKSGVTAAWTLNMQRVIRLE
                     PPLIVTADEVEQALHALRGAVASGYAKLGIL"
BASE COUNT          461 a         1026 c          921 g          514 t
ORIGIN      
        1 cgcgcacgcg ttcgaagcgc ggattccccg gtaccgacat attgccgcct tcggtgacga
       61 gcacgtccgg ccgctcgcgg gcgacccggc ggctgacgtc gtgcgggagc gacagctcgc
      121 acaccaccgc gccggtgcgc aaatcctcgg gttcgacgat ttcctgcgtc gacgaggttg
      181 ccgtcagcac cagctgcgcg cggcgcacgg ccgcacctac gtcggtcgtg tagctggatt
      241 cgcacggcag ctcgccggcg attcgctcgt gaaacttgcg cagccgcgtt tcgttgcgcg
      301 cgaccatcac gacgtggttt accttcggcg cgatcagctg gaggcacgcg ctgccgatcg
      361 aaccggtcgc gccgattacc accgcggtcg actcggcgac gtcgatgttc atctcttggg
      421 cggcacggaa gaaactctgc acgcctgcgc cgatcgtcag cgagttgccg gtggtcaccg
      481 gaatcggcga tcgctcgttg atcgttatcc cgccgtcgcc gaccactgcg gtgaacgcgc
      541 ccagcccggc aacctgcgcg ccgaggtccg ctccgatttg gattgccgcg aggatgcgcc
      601 cgtacacttc ctcccgcgga agctcgacca tctgttcggg cagcagcgac gcggagacga
      661 accagccttc gacttcgcga ccatcggggg cgcgcacgcc ggtgacgtgc accgccgccc
      721 acggcggcat ccactccatg atcttgcgca tgatcgcgtc gcctttaccc ttggcgccgg
      781 gctcgtaacg cgcgacgtcg tcgagcgaga gcggatgaac gacgaaacaa aatttattca
      841 cgggcgctcg cctccatcgc acggggagga ccggtgctcg gcaagcgcgg cgtacgactt
      901 tttcgcaaaa gccatggatg gcgttttcct ttggtcgttg cgaaaaagcg tccgagcagg
      961 agcgagccgg atgcgagcga cgagcgtccg cgcggcctag cgccggtgct tcctgggcga
     1021 tcgggcgagc cggttctgcg cgctagaggc gttggctgca tgcaagtcct cgatcacgtt
     1081 tgggagcctt cgccggttgc ggcggcttcg gcttggcgtt tgagggcggc gagcatcatc
     1141 tcactgttct ccacgacttt cttctgcagc gtcgggccga tcagctccgc cagcgtcggg
     1201 acgccgaaat cgtagtccac gcccagccgc acgtgcgtcg tcccgtcgcg ttcggcgaag
     1261 gtccacgatc cttcgaactt gtcgaggtcg ccctccagca gtttgtaatc gatccgcagc
     1321 gcttcgtcgt cgaagcggtc ttcttccgtc cactcgatgg gcgcctcctc gaccagcgtt
     1381 ttccagcgcg agatcacgcg gtcggggtgc cgctcgagca cggtgacggt ttcgacgtcg
     1441 ggcatgaact ccggaaatcg ctcctgatcc ttggcaagtt cgtacacgat tttggccggt
     1501 gcggcgatcg cgatcgcact ctcgacgtag ggcatgcgcg ctccgtgtct cctacaggat
     1561 acccagcttg gcgtatccgc tcgcgaccgc gccgcgcagc gcgtgaagcg cctgctcgac
     1621 ttcgtcggcg gtgacgatga gcggcggctc gaggcgaatg acccgctgca tgttcagcgt
     1681 ccaggcagcg gtgacgccgc tcttgagcat ctccgcgatg atccagccgc cgtagccttc
     1741 cgatgccagc tcgactccga ccatcaagcc cagaccgcgg gcctcgcgca cgaccgccgg
     1801 gaactcggcc gcgatcgcgc gcgcgccttc gagcagctgt tcgccgcgaa cgcgcgcgtt
     1861 ttcggccagg ccttcgtctt ccaacacgtc gatcgcggcg atcgccgccg tgcaagccag
     1921 ctcgccgccg ccgaacgtcg aagtatgcag cagcggcgcc ttgccgaagg cgcgctccca
     1981 agcgtccggc cgcgcgatga acgcgccgac cgggatcacg cctcccgaaa gccccttggc
     2041 gagggtcatc gcgtcgggca cgacgccttc gcgctcgcat gcgaaacggt aaccgcagcg
     2101 cccgagcccc gtttgcactt cgtcggcaac caacagcgcg ccgctgcgat cgcaggccgt
     2161 ccgtacggcg cgcaaatatc cgtccggcgg aacgttgacg ccgccttcgc cctgcaccgg
     2221 ctccaccacg aacgcagccg cgtcgctcaa taccgtttcg agctgctccg tctcgccgta
     2281 cgggatctgc gcgaaacctt cgagcagcgg gcggaagggc gtttgaaacg attcgcgtcc
     2341 gctcaccgat agcgcgccca tcgtctttcc gtgaaaggca ccgagcgtgc tgacgatctt
     2401 ggcgcgcccg gtggcagcgc gcgcgagttt gatcgcgccc tcgatggctt ccgtcccgct
     2461 gttgcaccaa aacgaaattc gtaaatcgcc gggtgcgatc tccgccagcc ggcgcgccgc
     2521 ccgtcccagc accggattga acatcgtctt gcccgagagc gacatcagct ccatctgctc
     2581 gcgcaccgcg tgcagcaccc gcggatgcga gtgtcccaac gtgaagacgc cgtaaccgcc
     2641 agcaaaatcg agatacgcct tgccgttctg atcccaaatc gtgcagccgc gcgcgcgcac
     2701 ttccgccggc gaacccgaca atttcatgac gcgcgcgagc ggcgggttga cgaactgccg
     2761 gtagttttcg aacgcctccg cggcaatgtc cgcgctgggc ggcgacggcg gtcgcaaact
     2821 cacgcggctg ccggagcggc ttcgagccgg tcgatcgttt cgccgatcac ctccatggcc
     2881 tcggcggccg cgtcgacgtg catgatgcgt tcgcgcagcg cc
//
LOCUS       DASUCJ010000676         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1413331, whole genome shotgun sequence.
ACCESSION   DASUCJ010000676 DASUCJ010000000
VERSION     DASUCJ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413331"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFU90_08050"
     CDS             <1..364
                     /locus_tag="VFU90_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5479770.1"
                     /translation="PPSACAAVLACIEILRAEPERRERLWAHGRRMRRDLKGLGFDVG
                     AAEAPIVPVIIGGDLQTFAFWRRLYEGGVFANPVISPAVPENSARIRTSYIATHTADQ
                     LDFVLDVFDNVGKHMGVI"
     gene            538..>1138
                     /locus_tag="VFU90_08055"
     CDS             538..>1138
                     /locus_tag="VFU90_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479771.1"
                     /translation="MIDRERHPFHRHAEVELFLARNASGRVVGRVAAARNKLHEETHS
                     EPVGFFGFFETERDPAIACALLDAAGAWLAARGLTTMRGPASFSLTEECGLLVDGFDT
                     PPMVMMAHNPPWYADVLEAAGMRKTKDLLAYLRGVYPPQERLVAFERIARERYDVTVR
                     PFDKSKFGEEVERIRTLYNAAWEANWGFVPMTEAEFNHIA"
BASE COUNT          194 a          363 c          375 g          206 t
ORIGIN      
        1 gccgccatcc gcgtgcgccg ccgtgctggc gtgcatcgag attctgcgcg ccgagcccga
       61 gcgtcgcgag cggctgtggg cgcacggtcg tcgcatgcgc cgggacctga agggcctggg
      121 ttttgatgtc ggcgcggcgg aggctccgat cgttccggtc atcattggcg gagacctgca
      181 gactttcgcc ttctggcgtc ggctgtacga gggcggcgtc ttcgcgaacc cggtgatctc
      241 gccggcggtg ccggagaatt cggcacgaat tcgaacttcc tacatcgcga cacacacggc
      301 ggaccagctc gatttcgtgc tcgacgtgtt cgacaacgtc ggcaagcaca tgggggtcat
      361 ctgaccactt cagcttccat cggcctcgcg gccggtctca ccaacgcgtc cgctcagcca
      421 ctggacatcc aaccggtccg caccgcggct gaccggaggg cgttcatcga gctgccgtac
      481 cggctctatc gtgacgatcc gctctgggtg ccgccacttc gaagtgacgt cgctgaattg
      541 atcgaccgcg agcgacatcc cttccaccga cacgctgaag tcgagctctt cctggcgcgc
      601 aacgcgagcg gacgcgtggt gggacgcgtg gcggcggcgc gaaacaaact gcatgaggag
      661 acgcactcgg agccggtcgg atttttcggc ttcttcgaga cggagcgcga cccagcgatc
      721 gcgtgcgcac tgctcgacgc cgctggtgcg tggttggccg cgcgcggact gacgaccatg
      781 cgcggaccgg cgagcttctc acttaccgag gaatgcggtc tgctcgtcga cggcttcgat
      841 acgccgccga tggtgatgat ggcgcacaat ccgccatggt atgccgacgt gctcgaggcg
      901 gccgggatgc gcaagacgaa agacttgctt gcctatcttc gcggcgtcta cccgccgcag
      961 gaacgattgg tcgcgttcga gcgcatcgcc cgcgagcggt acgacgtcac ggtacgtccg
     1021 ttcgacaagt cgaagttcgg ggaagaagtc gagcgaattc gaactctcta caacgcggca
     1081 tgggaggcaa actggggctt cgtgccgatg accgaagccg agttcaatca catcgcca
//
LOCUS       DASUCJ010000677         1436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_114342, whole genome shotgun sequence.
ACCESSION   DASUCJ010000677 DASUCJ010000000
VERSION     DASUCJ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114342"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VFU90_08060"
     CDS             <1..1085
                     /locus_tag="VFU90_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5479772.1"
                     /translation="SEYLRLSLVAPVRGGEAVSVVLGTAIGSVSFTGSVVAFAKLQEL
                     LSSKPFQFPLQRAFNALLVVVILALGVTMVMGGDTASTPWIVFAVALALGALLVLPIG
                     GGDMPVVIATLNALTGIAAAFTGLVLHNQILVAAGILVGASGTLLTLLMGKAMNRSLG
                     NVFFGGFGAATAAAGGPAGSAVAKTVRQTSAEDTAIALAYARKVVIVPGYGLAVAEGQ
                     HAVRELAQELEKRGVDVKFAIHPVAGRMPGHMNVLLAEANIPYDELVEMDQINGDFDS
                     TDVVLVVGANDVVNPAAREDPTSPIYGMPILDVDKARNVIVLKRSMGTGFAGIDNALF
                     YRDRTRMLFGDAKKSLTSLVEALKAV"
     gene            complement(1114..>1436)
                     /locus_tag="VFU90_08065"
     CDS             complement(1114..>1436)
                     /locus_tag="VFU90_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479773.1"
                     /translation="SGAAADTIWVIAYYRAALVGFVTNDTALGAPPTTVTGGVTPSAA
                     FGSCVALRTQVPQDVTVPFGFGCQRHNVATEMSGRITVPGGTLDVTVPSQTIAGVRLL
                     ANAP"
BASE COUNT          223 a          488 c          491 g          234 t
ORIGIN      
        1 tgtccgaata tcttcggctg tcgctcgttg cgcccgtgcg cggcggcgaa gcggtgagcg
       61 tcgtgttggg caccgcgatc gggtcggtgt cgttcaccgg cagcgtcgtc gcgttcgcca
      121 agctccagga actgctctcg agcaagccgt tccagtttcc cctgcaacgc gcgttcaacg
      181 ccctgctcgt ggtcgtcatt ctcgcgctcg gcgtcaccat ggtcatgggc ggcgataccg
      241 ccagcacgcc gtggatcgtg ttcgccgtcg cgctcgcgtt gggcgcgctg ctcgtgctcc
      301 ccatcggcgg cggcgacatg ccggtcgtca tcgcgacgtt gaacgcgctc acgggcatcg
      361 ccgcggcgtt cacgggcctg gtgctgcaca accagatcct ggtcgccgcg ggcatcctgg
      421 tgggcgcgtc gggcaccctg ctgaccctgc tcatgggcaa ggcgatgaac cgctcgttag
      481 gcaacgtgtt cttcggcggc ttcggcgcgg cgacggcggc cgccggcggc ccggcgggca
      541 gcgccgtcgc gaaaaccgtc cgccagacga gcgccgagga tacggcgatc gccctcgcgt
      601 acgcgcgcaa ggtggtgatc gtgcccggct acggcctcgc ggtggccgag ggccagcacg
      661 cggtgcgcga gctggcgcag gagctcgaga agcgcggcgt cgacgtgaag ttcgcgattc
      721 acccggtggc cggccgcatg ccgggccaca tgaacgtgct cttggccgaa gcaaacattc
      781 cgtacgacga gctggtcgag atggaccaga tcaacggcga cttcgacagc accgacgtcg
      841 tgctggtggt gggcgccaac gacgtggtga atcccgccgc gcgcgaggat ccgacgagcc
      901 ccatctatgg tatgccgatc ctcgatgtgg acaaagcgcg caacgtgatc gtgctcaagc
      961 gcagcatggg cacgggcttc gccggcatcg acaacgcgct cttctaccgc gaccgcacgc
     1021 gcatgctgtt cggcgacgcg aagaagtcgc tcacgtcgtt agtcgaggcg ctcaaggccg
     1081 tctaacgcga caccgtctca ctcccggccc acgctaggga gcgttggcga gcaagcgaac
     1141 gccggcaatc gtttgcgacg gcacggtaac gtccaacgtc ccacctggga ccgtgatccg
     1201 ccccgacatc tcggtcgcca cgttatggcg ctgacagccg aagccgaacg gcaccgtcac
     1261 gtcctgcgga acctgtgtcc ggagcgcgac gcacgagcca aacgctgctg atggggtgac
     1321 gccgccggtc acggtggtcg gtggcgcccc gagcgccgtg tcgttggtca cgaagccgac
     1381 tagcgccgcg cgatagtagg cgatgaccca gatcgtgtcc gctgcggcgc ccgacc
//
LOCUS       DASUCJ010000678         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_358830, whole genome shotgun sequence.
ACCESSION   DASUCJ010000678 DASUCJ010000000
VERSION     DASUCJ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358830"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU90_08070"
     CDS             <1..723
                     /locus_tag="VFU90_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEU5479774.1"
                     /translation="NATPPATTPFDPNDPTSYNQASPFTVYDSLGQSHSATVYYVNTG
                     PGQWTANMYVDGTSMGSQPVGFDANGVLTTPAGGQLTFSGFTPPNGANPMDITVDASA
                     ITQYGDTFDPGTITQNGYAPGKVTGIDIDQNGIVSARFSNGQTKALGQLALADFENAQ
                     GLKNVGNTQWVETAQSGQVIRGAAGSGDFGAVQSGALEDSNVDLTSQLVNMIKAQRNY
                     QANAQSISTDDKMTQTILNIRN"
     gene            754..>1050
                     /locus_tag="VFU90_08075"
     CDS             754..>1050
                     /locus_tag="VFU90_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEU5479775.1"
                     /translation="MDRSLYVAMTGAGQTLQAQSSVAHNLANANTTGFKADLAAFQSV
                     PVQGAGLKTRVNAVSQGLGADFSPGAETTTGRELDIAVRGQGWIAVQAPDGSEAY"
BASE COUNT          211 a          345 c          344 g          150 t
ORIGIN      
        1 aacgcgacgc cgccggcgac gacgccgttc gatccgaacg atccgacgag ctacaaccag
       61 gcttcgccgt tcaccgtgta cgactcgctc ggccagtcgc attcggcaac ggtctattac
      121 gtcaacacgg gacccggcca gtggacggcg aacatgtacg tcgacggcac gtcgatgggc
      181 tcgcagccgg tcggattcga cgcgaacggc gtgctcacga cacccgccgg cgggcagctc
      241 acgttctccg gattcacgcc gccgaacggc gcgaacccga tggacatcac ggtcgacgca
      301 tccgcgatca cgcagtacgg cgacacgttc gatcccggca cgatcacgca gaacggctac
      361 gcgccgggca aggtcaccgg catcgacatc gaccagaacg gcatcgtgtc ggcgcgcttc
      421 tcgaacggcc agacgaaggc gctcggccag ctcgcgcttg ccgatttcga aaacgcgcag
      481 ggcctcaaga acgtcggcaa cacgcagtgg gtcgagacgg cgcagtcggg ccaggtcatc
      541 cgtggcgccg cgggcagcgg tgatttcggt gcggtgcagt cgggcgcgct cgaagattcg
      601 aacgtcgatc tgacctcgca gctcgtcaac atgatcaagg cgcagcgcaa ctaccaggcg
      661 aacgcgcagt cgatctcgac cgacgacaag atgacgcaga cgattctcaa catccgcaac
      721 taagtgatct gagaagacgc cggggagaag atcgtggatc gctcgctgta tgtcgcgatg
      781 acgggtgcgg gacagacgct gcaggcgcag tcgtcggtcg cgcacaacct cgccaacgcg
      841 aacacgaccg gcttcaaggc cgacctcgcc gcgttccaga gcgtgccggt gcagggcgcc
      901 ggacttaaga cgcgcgtcaa cgcggtttcg cagggactcg gcgccgattt ttcgcccggc
      961 gccgaaacta cgacgggccg cgagctcgac attgcggtgc gcggacaagg ctggatcgcg
     1021 gtgcaggcgc cggacggttc cgaggcgtac
//
LOCUS       DASUCJ010000679         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1306134, whole genome shotgun sequence.
ACCESSION   DASUCJ010000679 DASUCJ010000000
VERSION     DASUCJ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306134"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1201
                     /locus_tag="VFU90_08080"
     CDS             44..1201
                     /locus_tag="VFU90_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5479776.1"
                     /translation="MTRARRTLTLAALAILVIGIGGWLMHQRSGSRAAVPKEAAAVPV
                     TVATVARHDIPVRLYAIGNVEPYTTVSVKARVDGQIVAVRFAEGDEVHQGAVLFEIDR
                     RPFEAQLAQVQANLVKDRAVLDHAAEQEKRYKDLLAQKFISPDAYGQVKANAESAAAQ
                     VRADQAAIEGVKLQLSYCTIRAPITGYAGKIMIQRGNLVKANDTNALVVINQVRPIYA
                     SFSIPEQNLDAVRRYLADGELAVTASVPNSGSAAVANQAGAPVTGKLTFIDNTTDVST
                     GTIKLKAEFPNTGKELWPGQFVNVVLTLTQEKNAIVMPSTAVQNGPDGQYVFVVGADG
                     HAALRKVKIERTESGDAVVASGLAPGERVVTAGQLRLAPGVAVTIDSAKGA"
BASE COUNT          238 a          451 c          448 g          203 t
ORIGIN      
        1 gagccgatcc ctgcatgcgg cccgcgccca ggagagtttc ggcatgacac gagcgcggcg
       61 cacgctgacg ctggccgcgt tggcgatact ggtgatcggc atcggcggat ggctgatgca
      121 ccagcgctcc ggctcgcgcg cggcggtccc gaaggaggcc gccgccgtgc cggtcaccgt
      181 cgcgacggtg gcgcgacacg acatcccggt tcgcctctac gccatcggca acgtcgagcc
      241 ctacacgacg gtctccgtca aggcgcgcgt cgacggccag atcgtcgccg tacgtttcgc
      301 cgaaggcgac gaagtgcatc agggcgccgt actgttcgag atcgaccgcc gaccgttcga
      361 ggcccagctc gcacaggtgc aggcgaacct cgtgaaggat cgcgcggtgc tcgatcacgc
      421 ggccgagcag gagaagcgtt acaaagatct cctcgcgcag aagttcatct cgcccgacgc
      481 ctacggccag gtgaaggcca atgcggagag cgccgcggcc caggtgcgcg ccgaccaggc
      541 ggcaatcgaa ggcgtcaagc tccagctcag ctactgcacg atccgcgcgc cgatcaccgg
      601 ctacgccggc aagatcatga tccagcgcgg caacctggtg aaggccaacg acaccaatgc
      661 gctggtggtg atcaatcagg tgcgcccgat ctacgcgtcg ttctcgattc ccgagcagaa
      721 cctcgacgcc gtacgcaggt atctcgccga cggcgagctc gcggtcaccg cgtcggttcc
      781 gaactcgggc agcgcagcgg ttgcgaacca ggcaggcgcg ccggtgacgg gaaagctcac
      841 cttcatcgac aacacgaccg acgtctcgac cggcacgatc aagctcaagg ccgaattccc
      901 gaacaccggc aaggagctgt ggccggggca gttcgtcaac gtcgtgctga cgctgacgca
      961 ggagaagaat gcgatcgtca tgccgtcgac cgccgtgcag aacgggcccg acggtcagta
     1021 cgtgttcgtc gtcggcgctg acgggcacgc cgcgctgcgc aaggtgaaga tcgagcgcac
     1081 cgagagcggc gacgcggtcg tcgcctcggg cctcgctccc ggcgagcgcg tggtcacggc
     1141 cggccagctg cggctcgcgc cgggcgttgc ggtgacgatc gacagcgcga agggcgcgtg
     1201 agcatttcgg cgctgttcgt tcgccggccg gtcatgacga tgctgatcat gatcggcatc
     1261 ctcattttcg gcatcgtcgg ttaccggctg ctcccggtga acgcgcttcc caacgtcgac
     1321 ttcccgacga ttcaggtcga
//
LOCUS       DASUCJ010000680         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_251767, whole genome shotgun sequence.
ACCESSION   DASUCJ010000680 DASUCJ010000000
VERSION     DASUCJ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251767"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="tadA"
                     /locus_tag="VFU90_08085"
     CDS             <1..508
                     /gene="tadA"
                     /locus_tag="VFU90_08085"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU5479777.1"
                     /translation="PPKPKVGGSSPLGRAMDDESCMRRALELAAVASARGDVPVGAVL
                     VRNGEIAAVGFNQKEAAHDPTAHAEIVAIRQAAARSGGWRLSGSTLYVTKEPCVMCAA
                     ACVAARIDRIVYGCPDPKGGGAGSVGNVASHEKLNHRVAVEGGVLGEEAAQMLRAFFK
                     ERRAEELS"
     gene            525..613
                     /locus_tag="VFU90_08090"
     tRNA            525..613
                     /locus_tag="VFU90_08090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Ser,seq:cga)
     gene            670..757
                     /locus_tag="VFU90_08095"
     tRNA            670..757
                     /locus_tag="VFU90_08095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Ser,seq:gga)
     gene            778..877
                     /gene="ffs"
                     /locus_tag="VFU90_08100"
     ncRNA           778..877
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFU90_08100"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            966..1451
                     /locus_tag="VFU90_08105"
     CDS             966..1451
                     /locus_tag="VFU90_08105"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEU5479778.1"
                     /translation="MTDAWQTFVNGLAPAVTSPIFVVGAVFTIAALVVYHLTTARSTT
                     GRAPARPGAPQAAAPPDLSDVRRRLAALEAAAEGALQHVGFVRFNAFPDVGSELSYAL
                     AVVDGKGDGFVVSSIYSREEVRTYAKAVSGFGTDKETSEEERRALAIAKEKSGRPRLR
                     G"
BASE COUNT          280 a          480 c          565 g          249 t
ORIGIN      
        1 gcctccgaag ccgaaggttg gtggttcgag cccactcggg cgcgccatgg atgacgagtc
       61 ctgcatgcgc cgcgcgctcg aactggctgc ggtcgcgagc gcgcgcggcg acgtgccggt
      121 cggagcggtg ctcgtccgca acggcgaaat cgcggcggtc ggattcaatc aaaaggaagc
      181 cgcgcacgat ccgacagcgc acgcggagat cgtcgcgatc cgccaggcgg ctgcgagatc
      241 gggcggctgg cggctgagcg gttcgacgct ctacgtgacg aaggagcctt gcgtcatgtg
      301 cgcggcggcc tgcgtcgcgg cgcggatcga ccggatcgtc tacggctgtc cggatccgaa
      361 aggcggcggc gcgggctcgg tcgggaacgt cgcaagccac gagaagttga atcaccgcgt
      421 ggcggtagaa ggaggagtgc tcggggaaga agcggcgcag atgctgcgcg cgttcttcaa
      481 agagcgacga gcagaagagc tgtcgtgacg cgaacgacgc atgcggagag gtggcagagt
      541 tggttgatcg cgctcgcctc gaaagcgagt aacggcttat acccgttcgt gggttcgaat
      601 cccaccctct ccggatcttc gtctcaagct cgtcaagccg acaccgtgat gcaaggcacg
      661 tcacctcgcg gagaggtgct ggagtggtcg atcaggcacg cctggaaagc gtgtaggcgc
      721 taaaaccgtc tcgagggttc gaatccctcc ctctccgatc gcatgggagg cgatgacggt
      781 caggacccgc gcgacgaaaa cccgctaatc ccgtcaggac cggaaggtag cagcggcgtg
      841 tgggaccttt cgggtgccgc ggttagtcct ggctcgagcc tccccttatg acatgtgtcg
      901 ctcgacacgg gcgggacaaa aggacaggtg agagttccgg tgcgaacctc ccgcaggcca
      961 acgtcatgac tgacgcgtgg cagacgtttg tgaacggctt ggcgccggcc gtcacgtcac
     1021 cgatcttcgt cgtgggcgcc gtcttcacga tcgccgcgct cgtcgtctac catctgacga
     1081 ccgcgcggtc cacgaccggt agggcgcctg cgcgcccggg tgcgccgcaa gcggcggcgc
     1141 cgccggatct gtcggacgtg cgccgacggc tcgccgcgct cgaagcggcc gccgagggcg
     1201 cgctgcaaca cgtcgggttc gtccgcttca atgcgttccc cgacgtcgga tcggaattgt
     1261 cgtatgcgct cgccgtcgtc gacggcaagg gcgacgggtt cgtggtttcg agtatctact
     1321 cgcgcgaaga ggttcgcacg tatgcgaagg ccgtgagcgg attcgggacc gacaaagaga
     1381 cgagcgagga agagcgccga gcgctcgcca tcgccaagga gaagtccggt cggccgcgtc
     1441 tgcgcggctg agggcacgga ggcccgcggg gctcgcgagc gccgcacgcg gcgcgacgtt
     1501 caacggggga gagacgtgct gaaggaactg ttctcatcga cgaagcggat cgtcatcaag
     1561 atcatcccgc acag
//
LOCUS       DASUCJ010000681         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1505081, whole genome shotgun sequence.
ACCESSION   DASUCJ010000681 DASUCJ010000000
VERSION     DASUCJ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VFU90_08110"
     CDS             <1..1761
                     /locus_tag="VFU90_08110"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5479779.1"
                     /translation="SAGVNAAISQQPLPIEFRLLLYRPRPWTPQDSLAVSLAVSIALA
                     DSWHDVLTRNDVWQRYGARGYDQYYPLSDGRYDVTRNGTPAAHETSAVVAIRLPPALA
                     GQTVRAGSNAWAAGGRRTSDGRALLANDPHLDLTIPGLWYLEDLRAPGIHVAGASIPG
                     APGILLGHNERVAWGATNADASAMSLFVAGRVPASNWRPERFKVRFAKDVTRAYYRSA
                     DAFGVPDAYDGGRLVLVRWPAFAGRSSAIATFLALDRASSVHAALRVLARYRGTAENF
                     VIAGDDGEVAYHLAGGVPLDPAWSRYVHPARELRSVYGEIPFERLPEAAPSRDGVIVS
                     ANNKMYGPGYAYRLSATFDPPYRAYRVAQLLRNRRRYDAGWFANMQLDTVSPVDAEFA
                     RLASAQGADRDEPGFERVTRALSAWNGSFSRRSREATIEHAVRIRLEADSPSLFALLE
                     RLRAGNDTDAEADVRGALYYASTLSQPWGRAGAVRVEHPLAPMRFGFLNGATLPGDGD
                     EYTIHLQEPGFSQSFRAVWEPGNWDAGGIAIPSGESGEPGSGHYDDLSGAWIAGRLEP
                     LPFGNAAVRAATRERLLLEP"
     gene            complement(1775..3037)
                     /locus_tag="VFU90_08115"
     CDS             complement(1775..3037)
                     /locus_tag="VFU90_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479780.1"
                     /translation="MRARTLTAFVLAAALTAVPPAAVAGVRAVAIVGSEGVTSPAAPA
                     ALNRYAARRWPAWHLPPGRLTPHGAVLATLFGAYYRSYYARAGLLPPSGCADASFVYV
                     RADLRPASQATAAALLSGLAPQCDLTVAASAAPPDPAFDPLPALGKADPAQSAAAVTA
                     AAGGDLTAIPATYRPALARLDGLLGCSGIDCTRIATLPSRVVIDGATGLASLHGPVDT
                     AAAAVDELLLEYAGALPGESVGWGRLDLDTLGGLLQLRVLRQLVEDRNPYAARLTASS
                     LAAAVTATLDQSVSGRRDAQTPAPPASRFVAFIGPETNLARLAGLLQLAWVVPGAQPD
                     ATLPGSALVFELHDADRRAGENAPFVQAFFVAQPLLAMRRASAGSGALAVMRLPLRIP
                     GCPDVRCPLRTFDSVVRAAVDPSFVARF"
     gene            complement(3047..4204)
                     /locus_tag="VFU90_08120"
     CDS             complement(3047..4204)
                     /locus_tag="VFU90_08120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5479781.1"
                     /translation="MRPELLAAVRDLNFTEPTPIQVLAIPPALEGRDVLASAETGSGK
                     SAAFGLALLQRLVDEPPGKTRALVLAPTRELAVQIADHLKQLAKHTRVTVAAVYGGVG
                     FKAQAAAFKRGAAIVVATPGRLLDHVAQGTARLGAVDVLVLDEADRMLDMGFLPDVRR
                     IIRSLPARRQTLFFSATLPPQISGLVREILHDPARVELAAKAKPVRTLSQTLYAVPQE
                     HKTDLLVELLKDNAIYTAIAFTRTKARANRLAAALGKHRIPVDLIHGDRSQSQRTRAL
                     DDFKRGKFRVLVATDIASRGIDVEQLGHVINFDVPLAAEDYLHRVGRTARAQAAGDAI
                     TFVSSDEEPLIRQIEYVLGHRLARTKNPLFPEASVEPPKPRVVYRSRARRR"
     gene            complement(4299..>4612)
                     /locus_tag="VFU90_08125"
     CDS             complement(4299..>4612)
                     /locus_tag="VFU90_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479782.1"
                     /translation="MQPALVFALLLGAFMVLAFVFLFGVEDLALQLTMTALVAGTMGL
                     LLGVIVVFSAPYAGALRISPSGWTYVIEKNGFEAVAAGARQSRAGAATLPIDQNGPSV
                     P"
BASE COUNT          738 a         1493 c         1669 g          712 t
ORIGIN      
        1 agtgcgggcg tcaacgcggc gatatcgcag cagccgctgc cgatcgagtt ccgtttgctg
       61 ttgtaccggc cgcggccctg gacgccgcaa gactcgttgg ccgtctcgct ggccgtctcg
      121 atcgcgctgg cggattcgtg gcacgacgtg ctgacccgca acgacgtttg gcagcgctac
      181 ggcgcacgcg gttacgacca atactatccg ctgagcgacg gccgttacga cgtgacgcgc
      241 aacggcacgc cggcggcgca cgaaacgagc gctgtggtgg cgattcggct accgcccgcg
      301 ctcgcaggcc aaacggtacg tgcggggagc aatgcttggg cggccggcgg acggcgcacg
      361 agcgacggac gggcgctgct ggccaacgat ccgcacctcg acttgacgat tccggggctg
      421 tggtacttgg aggatctgcg ggctcccggg atccacgtcg ccggcgcctc gattccgggg
      481 gcacccggta ttctgctcgg tcacaacgag cgcgttgcct ggggcgccac caacgccgat
      541 gcgtcggcga tgagtttgtt cgtggcgggc cgcgtgccgg cgtcgaactg gcggcccgag
      601 cgcttcaaag tgcgctttgc gaaagacgtt acgcgcgcgt actatcgcag cgcggatgcg
      661 ttcggcgttc cggatgccta cgacggggga cgcctggtgc tggtccggtg gcctgcgttt
      721 gcgggacgca gctcggcgat cgcgacgttc ctcgcgctcg atcgtgcgtc gagcgtgcac
      781 gcggcactcc gcgtgctggc gcggtatcgc ggaacggcgg aaaacttcgt gattgccggc
      841 gacgacggcg aggtcgccta tcacctggcg ggcggcgtac cgctggatcc ggcgtggagc
      901 cggtacgttc acccggcgcg cgagcttcga tcggtctacg gcgagattcc gttcgagcgg
      961 cttccggaag ccgcgccgtc gcgcgacggc gtgatcgtga gcgccaataa caagatgtac
     1021 ggcccgggct acgcttaccg cctgtcggca accttcgatc cgccctatcg cgcgtaccgg
     1081 gtcgcgcagc tgctgcgcaa tcgtcgccgt tacgacgccg gctggtttgc gaacatgcag
     1141 ctcgatacgg tgtcgccggt cgatgccgag ttcgcgcggc tggcgagcgc acagggtgcc
     1201 gatcgcgacg agcccggttt cgaacgcgtg acgcgcgcgc tgagcgcgtg gaacggatcg
     1261 ttctcgcggc ggtcgcggga agctacgatc gagcacgcgg tccgcatacg cctcgaggcc
     1321 gactcgccgt cgctgttcgc gctgctcgaa cggctgcgcg ccgggaacga tacggacgcc
     1381 gaagccgacg tgcggggcgc gctctactat gcgagtacgc tgtcgcagcc gtgggggcgc
     1441 gccggcgcag tgcgggtgga gcatccgctc gccccgatgc gttttggttt cctcaacggc
     1501 gcgacgctgc caggcgacgg cgacgaatat acgattcacc ttcaggagcc gggtttttcg
     1561 caaagcttcc gtgcagtctg ggagcccggt aactgggatg ccggcggcat cgccatcccg
     1621 agcggtgaat cgggcgaacc aggctccggt cactacgacg atctgagcgg cgcctggatc
     1681 gccggacgcc tcgaaccgtt gccgttcggc aacgccgcgg tacgcgcggc aacccgcgaa
     1741 cggctgttgc tcgagcctta accggcggcg gtttttaaaa ccgcgcgacg aacgacgggt
     1801 cgacggcggc gcggaccacc gaatcgaacg ttcgcagcgg gcagcgaacg tcggggcagc
     1861 cgggaattcg tagtggaaga cgcatcacgg caagcgcccc cgatccggcc gatgcgcgac
     1921 gcattgcaag cagtggctgc gctacgaaga aggcttgcac gaacggcgca ttctcgccgg
     1981 cgcgccggtc ggcgtcgtgc agttcgaaca ccagcgcgct gccgggcagc gtcgcgtcgg
     2041 gttgagcgcc cggcacgacc cacgcaagct ggagcagtcc ggcaagccgt gcgaggttcg
     2101 tttcggggcc gatgaaggcg acgaaacggc ttgccggcgg cgccggcgtt tgggcgtcgc
     2161 gccgtcccga aacgctttga tcgagcgtcg ccgtaacggc cgccgccagg ctcgatgcgg
     2221 ttaaccgcgc ggcgtacggg ttgcggtctt cgacgagttg acgaaggacg cgcaactgca
     2281 gcagcccgcc cagcgtgtcg aggtcgagcc ggccccaacc gaccgattct cccggcagcg
     2341 cgcccgcata ttcgagcagg agttcgtcga cggcggccgc cgccgtgtcg accgggccgt
     2401 gcagcgacgc aagcccggtg gcgccgtcga ttacaacgcg ggaaggcagc gtggcgatcc
     2461 gcgtacagtc gatgccggag catccgagca gtccgtcgag ccgcgcgagt gccggacgat
     2521 aggtcgccgg aatcgccgtc aggtccccgc cggccgccgc agtcaccgcg gccgcggatt
     2581 gcgcggggtc cgccttccca agcgcgggga gcggatcgaa cgccggatcg ggaggcgcgg
     2641 cggatgcggc aaccgtgaga tcgcattgcg gcgcgagtcc cgacagcagg gcggcggcgg
     2701 tcgcctgcga cgccggccgc aaatcggcgc gtacgtacac gaacgatgca tccgcgcagc
     2761 cggacggcgg cagcaatccg gcgcgcgcgt aatagctgcg gtagtaggct ccaaagagcg
     2821 tcgccagcac ggcgccgtgg ggcgtgagcc ggccgggcgg cagatgccat gccggccaac
     2881 ggcgggccgc gtaacgattc agcgccgccg gcgcggccgg tgaggtcacg ccttcgctgc
     2941 cgacgatcgc taccgcgcgc acgccggcga cggcggccgg cggaacggcc gtcaatgcgg
     3001 ccgccagcac gaacgccgtt agcgtacgcg cgcgcactct agcctgctat cggcggcgcg
     3061 cccgcgatct atacacgacg cgcggttttg gcggctcgac gctggcttcg gggaagagcg
     3121 gattcttggt gcgcgcgagc cggtgcccga gcacgtactc gatttgccga atcagcggct
     3181 cttcatcgga cgacacgaac gtaatcgcat cgccggcggc ttgcgcgcgc gcggtgcgcc
     3241 cgacgcggtg gaggtagtct tcggcggcca aaggcacgtc gaagttgatg acgtggccga
     3301 gctgctcgac gtcgatgccg cgcgaggcga tgtcggtcgc aaccagcacg cggaacttgc
     3361 cgcgcttgaa atcatcgagc gcgcgcgtgc gctgcgactg ggagcggtcg ccgtggatca
     3421 agtcgaccgg aatacggtgc ttaccgagcg ccgcggccaa ccggttcgcg cgcgctttgg
     3481 tgcgcgtaaa ggcgatcgcc gtgtagatgg cgttgtcttt gagcagctcg accagcaagt
     3541 cggtcttgtg ttcttgcgga acggcgtaga gcgtttggct cagcgtgcgc accggcttcg
     3601 ccttggcggc cagttcgacg cgcgccgggt cgtgcaaaat ctcgcgaacg agccccgaga
     3661 tttgcggcgg gagcgtcgcc gagaagaaga gcgtctgccg gcgcgccggc aacgagcgga
     3721 tgatgcgcct cacgtccggc agaaagccca tgtcgagcat ccgatcggct tcgtcgagca
     3781 ccaggacgtc gacggcgccg aggcgcgcgg tgccctgtgc cacgtgatcg agcaaacgtc
     3841 cgggcgtggc gacgacgatc gcggcgcctc gtttgaaggc ggccgcttgc gctttgaagc
     3901 cgacgccgcc gtagactgcc gcaacggtta cccgcgtgtg cttggccagc tgcttgagat
     3961 ggtccgcgat ttgcacggcg agttcgcgcg tcggggcgag taccagtgcg cgcgtcttgc
     4021 cgggcggctc gtcgacgagc cgctgcagca gcgcgaggcc gaaggcggcg gattttccgc
     4081 tgccggtctc ggcgctggcg agcacgtcgc ggccttcgag agccggggga atcgcgagca
     4141 cctggatcgg ggtgggttcg gtgaagttga ggtcgcggac ggcagcaaga agctccggcc
     4201 gcaagccgag tgaactaaag gttgaaatga tgggtcgcgt ctttcgatgg tgtgcgcggc
     4261 tcgatcgcag ttcgaaagat cgcgcgggag ttgccctact atggcacgga cggcccgttt
     4321 tggtcaatcg gaagggtcgc agcgccggct cgggactggc gagcgcctgc cgcgaccgcc
     4381 tcgaaaccgt tcttctcgat gacgtaggtc catccgctcg gcgagatacg cagcgccccg
     4441 gcgtagggcg cactgaagac gacgatcact cccaaaagga ggcccatggt gccggcgacc
     4501 agcgcggtca tcgtcagctg cagcgcgaga tcctcgacgc cgaacaaaaa gacgaaggcc
     4561 aacaccatga acgcgccgag cagcagggca aataccagcg ccggctgcat cc
//
LOCUS       DASUCJ010000682         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_664398, whole genome shotgun sequence.
ACCESSION   DASUCJ010000682 DASUCJ010000000
VERSION     DASUCJ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664398"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFU90_08130"
     CDS             <1..255
                     /locus_tag="VFU90_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479783.1"
                     /translation="QAREEAGFLTDAAFSKVLEDLRDARAPLVEGDAGELELTPLGRR
                     VLSGEADWLHTLPIDRWIGGVHLVPQLNVRWDDGEEKFVE"
     gene            323..697
                     /locus_tag="VFU90_08135"
     CDS             323..697
                     /locus_tag="VFU90_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5479784.1"
                     /translation="MSHDELSIDLKSEDHGETLVFRLRGSLDLATSPTLRAALGEAAE
                     KGTKSIVVDLTQLEFLDSTGLGALIGAHRRAAERGGSVRLIVNDGPIARLLNITGLIR
                     VLAVYHTLDGALNDQDRVSAGV"
     gene            complement(694..>1107)
                     /locus_tag="VFU90_08140"
     CDS             complement(694..>1107)
                     /locus_tag="VFU90_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5479785.1"
                     /translation="GERVRDVLEVLDAMQLDPPAGKVVGAVTYQEPCHLAHAQHVTGA
                     PRRLLAKIPGLRLVEMDESAVCCGSAGIYNLANPEMAAALQRRKTDNVVRTRARIVAT
                     ANPGCALQVAAGLRAAGYQARVAHVVELLDEAYRV"
BASE COUNT          191 a          348 c          377 g          191 t
ORIGIN      
        1 caggctcgcg aggaagcggg ttttctaacc gacgcggcgt tttccaaggt tctcgaggac
       61 ctgcgcgacg cacgcgcgcc gctggtcgaa ggcgacgccg gcgagcttga gttgacgccg
      121 ctcgggcggc gagtgctctc cggggaagcg gattggctgc acacgcttcc gatcgatcgc
      181 tggatcgggg gcgtgcacct ggttccgcag cttaacgttc gctgggacga cggcgaggag
      241 aagttcgtcg agtgattcga cgcccacgcc cagcgggacc ggcacaacag gcacgcgggg
      301 catggcggag aagacgaggt ctatgtctca cgacgaactg agcatcgact taaagtcgga
      361 agatcacggc gaaaccctcg ttttcaggct ccgcgggagt ctcgatttag ccacgtctcc
      421 caccctgcgc gcggctctgg gcgaggctgc cgaaaaggga acgaagtcga tcgtcgtcga
      481 tctcacgcag ctcgaatttc tggattcgac cggactcgga gcgctgatcg gcgcgcatcg
      541 ccgggccgcc gaacgcggcg gctccgtgcg cctgatagtg aacgatgggc cgatcgcgcg
      601 cctgctcaac atcaccggac tgattcgcgt gctcgccgtc tatcacacgc tggacggcgc
      661 cctgaacgac caggatcgcg tcagcgccgg cgtctagacg cgatacgctt cgtcgagtaa
      721 ttcgacgacg tgcgcgacgc gcgcctgata gccggcggcg cgaagcccgg cggcaacttg
      781 aagcgcacat ccgggatttg cagtcgcgac gatacgggcg cgggtgcgta cgacgttgtc
      841 ggtcttgcgg cgctgcagcg ccgcagccat ctcgggattg gccagattgt agattccggc
      901 gcttccgcaa cagaccgcgc tttcgtccat ttcgaccagg cgtaggcccg gaattttcgc
      961 aagcaggcgg cgtggcgcgc cggtaacgtg ctgcgcgtgc gccaggtgac acggttcttg
     1021 atacgtgacg gcgccgacga ctttgccggc cggcgggtcg agctgcatcg catcgagcac
     1081 ctcaagtacg tcgcggacgc gctcgcc
//
LOCUS       DASUCJ010000683         1360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_358895, whole genome shotgun sequence.
ACCESSION   DASUCJ010000683 DASUCJ010000000
VERSION     DASUCJ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358895"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFU90_08145"
     CDS             complement(<1..511)
                     /locus_tag="VFU90_08145"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5479786.1"
                     /translation="MQKTALITGITGQDGSYLAELLLQKNYRVVGMTRRTSTEVHERI
                     EHIVDDIEIVSGDLLDQTSMTSIVNDVRPNEVYNLAAQSFVPTSWNQPVLTGEFTALG
                     VTRVLEAIRHVDPSIRFYQASSSEMFGKVCEVPQRETTPFYPRSPYGVAKVYGHWITV
                     NYRESYQMFA"
     gene            complement(521..1282)
                     /locus_tag="VFU90_08150"
     CDS             complement(521..1282)
                     /locus_tag="VFU90_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HEU5479787.1"
                     /translation="MGRPVKPYGDTMNDGKVQMSFTLPVEWSEAADEAARQLVAKMGF
                     ADVQVAEGRRIADGFSLFVVYASTPQSIDVDAIKAPSARAHAMTLEEIDAYVAERIGR
                     KIRVAGACIGTDAHTVGIDAILNMKGYKGDYGLERYHWFEVYNLGSQVAPEDLVRFAK
                     ERDVDVLLVSQVVTQKGSDVKNFTELVELLEAEGLRDRVVLICGGPRVTNLMATELGY
                     DAGFGRGTLPSEVAAFAAVTLAERRAREAHLSSIH"
BASE COUNT          237 a          426 c          431 g          266 t
ORIGIN      
        1 aggcgaacat ttgatacgac tcgcggtagt tgaccgtaat ccagtgcccg tagacctttg
       61 cgaccccgta cggactgcgc ggatagaacg gcgtcgtctc gcgctgcgga acctcgcaga
      121 cctttccgaa catctccgag ctggaggcct gatagaaccg gatcgaggga tcgacgtgcc
      181 ggatcgcttc caacacccgg gtgacgccca gggcggtaaa ctcgccggtg agcaccggct
      241 gattccaaga ggtcggaacg aacgattgag ccgcgagatt gtaaacctcg ttcggccgga
      301 cgtcgttgac gatcgacgtc atcgacgtct gatccagaag atcgcccgac acgatctcga
      361 tgtcgtcgac gatgtgttcg atgcgctcgt ggacctcggt gctggtccgg cgcgtcattc
      421 cgacgacgcg gtagttcttt tgcaagagca gctcggcgag gtacgagccg tcctggccgg
      481 tgattccggt gatgagtgcg gtcttctgca aacgtcgctc ctagtgaatt gacgaaagat
      541 gggcttcgcg cgcccggcgt tcggccagcg tgacggcggc aaaggcggct acctcgctgg
      601 gcagcgtgcc gcggccgaag ccggcgtcgt atcccagttc cgtcgccatc agattggtga
      661 cgcgcggtcc gccgcaaatc agcaccacgc ggtcgcgcag tccttcggcc tccagcagct
      721 cgacgagttc cgtgaagttc ttgacgtcgc tgcccttttg cgtgacgacc tgagaaacga
      781 ggagtacgtc gacgtcccgt tccttggcga aacgcaccag atcttcggga gcgacctgac
      841 tgccgagatt gtagacttcg aaccagtggt agcgctcgag accgtaatcg cccttgtagc
      901 ccttcatgtt caaaatcgcg tcgatcccga cggtgtgcgc gtcggtgccg atgcaggcgc
      961 cggctacgcg gattttccgg ccgatccgct cggccacgta ggcgtcgatc tcttccaggg
     1021 tcatcgcgtg tgcgcgcgcc gacggcgcct tgatggcatc gacgtcgatc gactgcggcg
     1081 tgctcgcgta cacgacgaat aacgagaaac cgtcggcgat ccggcggccc tcggcgactt
     1141 gtacgtcggc gaagcccatc ttggctacga gctgacgggc ggcttcgtcg gcggcttcgc
     1201 tccactcgac cggcagcgtg aacgacatct gcactttccc gtcgttcatc gtatcgccgt
     1261 acggtttgac cggccggccc attaggcggg aaccggctcc ggccgcagcg cgtcttcgaa
     1321 gggattgcta tagccggcgg cgcgttcgaa cacgccgtcg
//
LOCUS       DASUCJ010000684         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_496141, whole genome shotgun sequence.
ACCESSION   DASUCJ010000684 DASUCJ010000000
VERSION     DASUCJ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496141"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFU90_08155"
     CDS             <1..1000
                     /locus_tag="VFU90_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEU5479788.1"
                     /translation="VIGIYTLLVGFNLAAWAWALSAFHAYPLLLGTALLAYGFGLRHA
                     IDADHIAAIDNVARKLMHDGKKPLTVGFFFSLGHSTVVVLASVAIAVTAMALESRMST
                     LHATGSIIGTLVSALFLLAIAVMNLFILAAVWRAFHRVRAGGAFGDEDLNVLLAGRGL
                     IARILRPLFGMVRRSWHMYPLGLLFGLGFDTATEIGLLGISAAEAAKGMPIWSILVFP
                     ALFTAGMSLVDTTDSILMIGAYGWTFVKPIRKLYYNLTITFVSVVVALLVGGIEALGL
                     VGHRFALDGAFWRAVGALNEHFGMLGYLIVLVFVVSWAASVLIYRLKRYDELEPSA"
     gene            complement(1030..2055)
                     /locus_tag="VFU90_08160"
     CDS             complement(1030..2055)
                     /locus_tag="VFU90_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU5479789.1"
                     /translation="MTLHRFALAAAAIATLAAVPAPGPGYHIIKTYTLGGEGGWDYLA
                     YDSVGHRLFISRQNRVMVVDPATGRQLGEIPGFERSHGIAFDYKTGHGFATSGGDSSV
                     IMFDLKTLRVLGRTTAAPDADAILFDPATGRVFTFNGDSKSSSVIDGQSGKKIGTVDL
                     GAGPEFGVSDGAGKVYVNLEEEGEVAEIDAKTLKVTRKWPMGSCKGATGLAIDRAHHI
                     LFSGCRTKVMAISNAAEGKLITTLPIGAGVDATRYDAGTGDAFASTGDGTLTVIHEDS
                     PDRFSVVETDTTMRGARTMEIDEQNHRVFTVSAKFGPPPAESTAQNPRRRPPMLPDSF
                     TLLILDR"
     gene            2271..3638
                     /locus_tag="VFU90_08165"
     CDS             2271..3638
                     /locus_tag="VFU90_08165"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU5479790.1"
                     /translation="MATRLTEREPEAPDLRAAERGWRRARVTPSLAEAYRTIAIPGVS
                     RWRKALAFAGPGFLVAVGYMDPGNWATSLAGGSAFGYSLLSVVVISNLLAILLQALAA
                     KLGIATGRDLAQAVRDSYSRPTVIMHWLLCEVAIAACDLAEVIGSAIALNLLFRIPVV
                     VGVLITGLDVLVILVLQHRGYRTLEALVIALIGTIGVCFVFEIVVSRPDLAAAARGML
                     PSPEIIRNPAMLYLAIGILGATVMPHNLYLHSSIVQTRRYDETPRGKREAIRYAVLDS
                     TAALMGALFINAAILVVAAATFHRFGHTEVAEIQDAYKLLTPLLGVGGASTVFALALL
                     ASGQNSTLTGTLAGQIAMEGFLDIRMRPWLRRLLTRGIAIVPAVIAAAYFGESGTAKL
                     LILSQVILSMQLSFAVFPLVIFTSNKAKMGEYVNPAWLKSLAYAVAFVIAGLNVWLLA
                     QLVRR"
BASE COUNT          546 a         1238 c         1231 g          720 t
ORIGIN      
        1 cgtcatcggc atctacaccc tcctcgtcgg cttcaacctg gcggcgtggg cctgggcgct
       61 ctcggctttt cacgcgtatc cgctgctgct tggcacggcg ctgctcgcgt acgggttcgg
      121 gcttcgccac gcgatcgacg ccgatcacat cgcggccatc gacaacgtcg cgcgcaagtt
      181 gatgcacgat ggcaagaagc cgctcacggt cggcttcttc ttctcgctcg gacattccac
      241 cgtggtcgtg ctggcgtcgg ttgccattgc ggtcacggcg atggcgctcg agtcgcggat
      301 gtcgacgctg cacgcaacgg gcagcatcat cggcacgctc gtgtcggcgt tgttcctgct
      361 cgcgatcgcg gtgatgaacc tgttcattct cgccgcggtt tggcgcgcgt ttcaccgcgt
      421 gcgtgcgggc ggcgcctttg gcgacgaaga tttgaacgtg ctgctcgcgg gtcgcggatt
      481 gattgcgcgc atcctgcgcc cgttgttcgg catggtgcgc cgcagctggc acatgtaccc
      541 gctcggcctg ctcttcggac tcggcttcga caccgccacc gagattggtt tgttaggcat
      601 ctcggccgcg gaagcggcga agggaatgcc gatctggtcg attctcgtgt ttcccgcgct
      661 gttcaccgcg ggcatgagcc tcgtcgacac caccgatagc attctcatga tcggcgcgta
      721 cgggtggacc ttcgtcaagc cgatccggaa gttgtactac aatctcacca tcacgttcgt
      781 gtcggtggtg gtggcgttgc tggtgggcgg gatcgaagcg ctcggcctcg tgggccatcg
      841 gttcgcgctc gacggcgcgt tctggcgcgc cgtcggagcg ctcaacgaac acttcgggat
      901 gctcggctac ctgatcgtgc tcgtgttcgt ggtcagctgg gccgcatccg tgctcatcta
      961 ccgcctcaaa cgctacgacg agctggagcc gagcgcctaa cgggaggcac gcccgttagg
     1021 cgcggctggt cagcggtcca ggatgaggag cgtgaacgaa tcgggcagca tcggcggccg
     1081 gcgccgcgga ttctgcgccg tcgactcggc cggcggcggc ccgaacttcg ccgacaccgt
     1141 gaacacgcgg tggttctgct cgtcgatctc catcgtgcgc gcgccgcgca tcgtggtgtc
     1201 ggtctcgacc acgctgaatc tgtccggcga atcctcgtgg atcaccgtga gcgtgccgtc
     1261 gccggtcgag gcgaacgcgt ctcccgttcc cgcgtcgtac cgcgtcgcgt cgacgccggc
     1321 gccgatcggc agcgtggtga tcagcttccc ctcggccgcg ttcgagatcg ccatcacttt
     1381 ggtgcggcat ccgctgaaca gaatgtgatg tgcgcggtcg atggccaatc cggtggcgcc
     1441 cttgcacgaa cccatcggcc acttgcgcgt cactttgagc gtttttgcgt cgatctcagc
     1501 gacctcgccc tcctcctcga gattcacgta gaccttgccg gcgccgtcgg acacgccgaa
     1561 ttccggcccg gcgccgaggt cgacggtgcc gatcttcttg cccgactggc cgtcgatcac
     1621 cgaggaggat ttcgagtcgc cgttgaacgt gaacacgcgg cccgtcgccg gatcgaacag
     1681 gatcgcatcg gcgtccggcg cggcggtcgt cctgcctaac acacgcagcg tcttcaggtc
     1741 gaacatgatc acggagctgt cgccgcccga cgtcgcgaat ccgtggccgg tcttgtagtc
     1801 gaacgcgatg ccgtgcgagc gctcgaagcc ggggatttcg ccgagctgcc ggcccgttgc
     1861 cggatccacg accatcaccc ggttctgccg cgaaatgaac aggcgatggc cgacggaatc
     1921 gtaggcgaga tagtcccagc cgccctcgcc gcctaacgta tacgtcttga tgatgtggta
     1981 gcccgggccg ggtgccggga ccgccgccag ggtcgcgatc gccgccgcgg ccagtgcgaa
     2041 tcggtgcaat gtcatgtgga cgagcctcgc gcgtacgtga gaggtcggtg ctccggtcgc
     2101 gcgccgctcg acgcgtgacc gcgcagagaa tagtagaggc ggcacctgtc acgccgatta
     2161 cacgacgcgc gcggaccggc gcccgctgcc gggcgcgaac gttttgtgaa agccggattt
     2221 gcattttcgt atgcactaaa ttattgatta gccatgcgag aaatggcgct gtggcgaccc
     2281 ggctgaccga gcgagagccg gaggctcccg acctgcgcgc cgcggagcgc ggctggcgtc
     2341 gcgcgcgcgt cacgccgagc ctcgccgagg cgtatcgcac gatcgcgatt cccggagtca
     2401 gccgatggcg caaggcgctg gcgttcgccg gacccggctt tctcgtcgcc gtcggctaca
     2461 tggatccggg caactgggcc accagcctcg ccggcggctc cgccttcggc tattcgctgc
     2521 tcagcgtcgt cgtcatttcg aacctgttgg ccatcttgct gcaggcgctg gcggcaaagt
     2581 taggcatcgc caccggacgc gacctcgcgc aggcggtgcg cgacagctat tcgcggccga
     2641 cggtcatcat gcactggctg ctctgcgagg tggccatcgc cgcgtgcgac ctggccgaag
     2701 tcatcgggtc ggccatcgcg ctgaatctgc tgttccggat tcccgtcgtc gtcggcgtgc
     2761 tgatcaccgg gctcgacgtt ctcgtcattc tcgtgctgca gcaccgtggc tatcgcacgc
     2821 tcgaggcgct ggtcatcgcg ctcatcggca ccatcggcgt gtgcttcgtg ttcgagatcg
     2881 tcgtgtcgcg gccggatctc gccgcggccg cgcgcggcat gctgccgtcg ccggagatta
     2941 tccgcaaccc ggccatgctg tacctcgcga tcggcatcct cggtgccacg gtgatgcccc
     3001 acaacctcta tctgcactca tctatagtgc agacgaggcg atacgacgaa acaccgcgcg
     3061 gcaaacgcga agcgatccgg tacgccgtgc tcgactcgac ggcggcgctc atgggcgccc
     3121 tgttcatcaa cgcggccatt ctcgtcgtcg cggcggcgac cttccaccgg ttcggccaca
     3181 cggaagtggc ggagatccag gacgcgtaca agctgctcac gcctctgtta ggcgtgggcg
     3241 gagcgagcac cgtgtttgcg ctcgcgctcc tcgcgtcggg gcagaattcg acgctcaccg
     3301 gcacgctggc cggccagatc gccatggaag gcttcctcga catccgcatg cggccgtggc
     3361 tgcgccggct gctcacgcgt ggcatcgcca tcgtgccggc ggtgatcgcg gcagcgtatt
     3421 tcggcgagag cggcaccgcc aagctgctca ttctgagcca ggtgatcttg agcatgcagt
     3481 tgtcgttcgc cgtgttcccg ctggtgatct tcacgtcgaa caaggcaaag atgggcgaat
     3541 acgtcaatcc ggcgtggctc aagagtctcg cgtatgccgt ggcattcgtg atcgcggggc
     3601 tcaacgtctg gctgctcgcc caactggtga ggcgttgatg gccgcgcgcg cgaagggtcg
     3661 gaagaccggc gccggatcgc cggccggcga ggccgcgaag cttcccgatc tcacggcgcc
     3721 ggtcgaggac tacct
//
LOCUS       DASUCJ010000685         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1306208, whole genome shotgun sequence.
ACCESSION   DASUCJ010000685 DASUCJ010000000
VERSION     DASUCJ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306208"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..835
                     /locus_tag="VFU90_08170"
     CDS             320..835
                     /locus_tag="VFU90_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479791.1"
                     /translation="MTMQMRRAGNWAKRTLTSRGSTVSMASAFIIAVAVLTFSAFRMT
                     SGASHNESRCPTGVAMDGTHPFGVLVDSIGNMQRGADMPVRYDVCGLNEGTHIKSRLT
                     VARTDGGRKGAADRMQATFDDNATGIGTRLHHVVPVGELPAGSYRLTIVVIDERGRRR
                     DTESTLRILAH"
BASE COUNT          169 a          359 c          344 g          167 t
ORIGIN      
        1 ggtggcaacg ggggagcatc gccgctggtc tccgacaccg gcgcctatcc caacggcgac
       61 tcggcccgcc gctgcccttc cggcggcgca gccaagcccc ttcggatcct acgaagggcc
      121 ccagccgtcg ccggccctcg atgcggctat cgatgccagc gttcagctgc tgaagcgtca
      181 caagccgaaa ctgcgcgaga tcggcgccgt cgctcggcgc gcaggacggg gattgtcttc
      241 cattgccggc cgtgtgcgac gggcgttcca gcgcggcgag cacgcgacga cgcctggcgg
      301 ccgcgttgca ctgggcagca tgacgatgca gatgcggcgc gccggcaact gggcgaaacg
      361 cacgctcacc tcgcgaggat cgacggtatc gatggccagc gcgttcatca tcgccgtggc
      421 cgtactcacc ttctcagcct ttcgcatgac gtcgggcgcg agccacaacg agtcgcgctg
      481 tccgaccggc gtcgcgatgg acggcacgca tccattcggg gtgctcgtcg actcgatcgg
      541 gaacatgcag cggggcgccg acatgccggt gcgctacgac gtgtgcgggt tgaacgaagg
      601 aacacacatc aagtcacgcc tgacggtcgc ccgcaccgac ggaggccgca aaggcgccgc
      661 ggatcgcatg caagcgacgt tcgacgacaa cgcgactggt atcggcacgc gcctgcatca
      721 tgtcgtgcct gttggtgagc tgccggcagg ctcgtatcgc ctgacgattg tcgtcatcga
      781 cgagcgagga cgacgccgcg ataccgaatc gacgctgcgc atcctcgcgc actgatcact
      841 gcccgatcca gggcagccac cagaatcgct tcttctttcg cgctgtcgcg cggccgctcc
      901 ggatgacgcg ttccggcggg gcgtgtttga cgtctccata cgccacgctg atgacgcgcg
      961 tcgtctttcc cggaagcggc ggaatctgca gtcgcgcgct tcccggagcg gccggcaaca
     1021 ttggcgcgtt cgacgcgcc
//
LOCUS       DASUCJ010000686         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_99213, whole genome shotgun sequence.
ACCESSION   DASUCJ010000686 DASUCJ010000000
VERSION     DASUCJ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99213"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFU90_08175"
     CDS             <1..870
                     /locus_tag="VFU90_08175"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5479792.1"
                     /translation="GSYLKSLVSPSALLDANARFESTTWTATMVGPPLGGALICAFGP
                     VTTLIADAVSYLLSALGITAIPGREARPAVTPRRTRGEVLEGWRYILGHRALRALLAN
                     TMLFNGLIMATAPLIAVLMLGRLEIAPWQYGIAFAAPCVGGLIGSRAARRLVALYGGH
                     CVLRWSGALRACWLVALAFIPGGVPGVVFVASVQFGLVTTCAVFNPVFATYRLEQTPI
                     DRLARTLAAWTITNKASVAALTAIWGALAAFAGVRLAIGVAGLIVLVTPLLLPRRQHL
                     ASSERSEELVAAA"
     gene            complement(958..>1272)
                     /locus_tag="VFU90_08180"
     CDS             complement(958..>1272)
                     /locus_tag="VFU90_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479793.1"
                     /translation="VAVTEGLVRTGVLNRDLAVQDVAPLTSLGVVVSQAARRPLVRPC
                     VDWTERRYHVAGALPAAMTTAFFERGWLARVGRGRAVRLTDAGRVELRWLIAEPAAAQ
                     PA"
BASE COUNT          175 a          425 c          444 g          228 t
ORIGIN      
        1 ggaagctatc tgaaatcgct cgtctcgccg tccgctttgc tcgatgcaaa tgccaggttc
       61 gaatccacca cctggacggc gacgatggtc gggccacccc tcggcggagc gctgatctgc
      121 gcctttgggc cggtcacgac actgatcgcc gacgccgtca gctacctgct ttcggcgttg
      181 gggatcaccg cgattccggg ccgcgaggcg cgccctgccg tgacgccgcg ccgcacgcgc
      241 ggcgaggtgc tcgaagggtg gcgctacatc ctgggccatc gggccttgcg cgcgcttttg
      301 gcgaacacaa tgctgttcaa cggcttgatc atggcgaccg cgccgttgat cgcggtgctg
      361 atgctgggcc ggctcgaaat cgccccgtgg cagtacggaa tcgcgtttgc cgccccgtgc
      421 gtcggcggtc tgatcgggtc gcgcgccgcg cgcaggctcg tggcgctgta tggcggccac
      481 tgcgtgctgc gttggtcagg tgccttgcgc gcctgctggt tggttgctct cgcgttcatt
      541 ccaggcggcg tccccggcgt ggtcttcgtc gcgagcgttc agttcgggtt ggtgacgacg
      601 tgcgcggttt tcaatccggt gttcgcgacc taccggctcg agcaaacacc gatcgatcgg
      661 ctcgcccgaa cgctcgcggc ctggacgatc accaacaagg cgagcgtggc cgcgctgacc
      721 gcgatctggg gcgcgctcgc ggctttcgcc ggcgtacggc tcgcgatcgg tgttgccggc
      781 ttgatcgtgc tggtcacccc gctcttgctg ccgcggcggc agcacctcgc atcgtccgaa
      841 cgtagcgagg aacttgtcgc cgccgcctag ccgcacacga ttccggtgac gcttgctacc
      901 ggatttcgtg agtaagcgtc caaagaacga gctcaggcgg gttgcgcgag aaggggctca
      961 ggcgggttgc gcggcggcgg gttctgcgat gagccagcgc aactcgacgc ggcccgcgtc
     1021 ggtcagccgg acggcgcgtc cacggcccac ccgcgcgagc caacctcgtt cgaagaacgc
     1081 ggtcgtcatc gcggcgggca gcgcgccggc gacgtggtag cggcgctccg tccagtcgac
     1141 acagggccga acgagcggac gacgtgctgc ctgcgacaca acaacgccga gtgatgtcag
     1201 cggtgcgacg tcttgcaccg cgaggtcgcg gttgaggacg cccgttcgca cgagcccttc
     1261 ggtgaccgcg ac
//
LOCUS       DASUCJ010000687         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1031419, whole genome shotgun sequence.
ACCESSION   DASUCJ010000687 DASUCJ010000000
VERSION     DASUCJ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031419"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFU90_08185"
     CDS             <1..550
                     /locus_tag="VFU90_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479794.1"
                     /translation="YELADRPDDAVVAAERAVSTDSMSAAAAAELGDALYYARRYDDA
                     LSQLMKVVALRPPLRRTAFYLDEVYLVKHRWKEAIDVMRPDTSDRNSRGLIGYALARS
                     GRERDARRLLQRMLATKSTSAAAVAEVYIGLRRYDSAFVWLDRSFDDHSLHPMIMGPL
                     FDDVHARPEFRRVRKRLGLPPG"
     gene            complement(645..1004)
                     /locus_tag="VFU90_08190"
     CDS             complement(645..1004)
                     /locus_tag="VFU90_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479795.1"
                     /translation="MIGIIAAAAACGGSNSSITGTTKNPPPTPMGGANITIQDFSFSP
                     AVDTVKVGTTVTWMNNGPSEHSTTSDTGIWDSGGLMPTGATFQHTFNQTGSFPYHCTF
                     HPPSLYPNFVGTIVVTN"
     gene            1478..2563
                     /locus_tag="VFU90_08195"
     CDS             1478..2563
                     /locus_tag="VFU90_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5479796.1"
                     /translation="MRWSWKLGRVAGIELRVHATFLLLLAWAALTYYRLGGTTGAAVR
                     GVVFTLALFASVVLHELGHAVAARRFGVPTRDITLLPIGGVARLAYIPRQPRQELVIA
                     LAGPLVTLLIVLALYGIGLGFSLPNGTIDAALGARGGFVGQLMWANVFLLVFNLLPAF
                     PMDGGRVLRAALALHGDYAKATNAAARAGRAFALVFGLVGLLYNPFLVLIALFIWLGA
                     AGEAADERLHSSLDGVPVEQLMIRDVQTLTPRDTLGVALRHVLAGFQHDFPVIDNGNL
                     VGILTRDALVKALARHGENVLVDEVMDRGFRTAAPGEFVEGALARLRDSDCRTIPVMA
                     DHRLQGVLTLDNIGEFVMINSALHGAM"
     gene            3004..3699
                     /locus_tag="VFU90_08200"
     CDS             3004..3699
                     /locus_tag="VFU90_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEU5479797.1"
                     /translation="MHAPFVDRREAGRALAGKLREYVDQPNVLVLGLPRGGVPVAYEV
                     AVALGAPLDVFVVRKLGVPGHEEMAFGAIASGGVRVLDPVVIAQLRLSDAAIDAVTRE
                     ERMELEQREELYRPERQFSSVAGRTVILVDDGVATGASMYAAVRALRQERPAKIVVAT
                     PVASADAIRALANVADECVCVMRPVIFQAVGLWYLHFDQTSNDEVRRLLREARDRPIG
                     LTAAGSAPPGADA"
     gene            3824..>4081
                     /locus_tag="VFU90_08205"
     CDS             3824..>4081
                     /locus_tag="VFU90_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479798.1"
                     /translation="MTTQQETIGLRIPARGVTLDGDLIAPEMPAGIIVFAHGSGSSRL
                     SPRNRQVAAALRASNFATLLFDLLTPAEEAMDVDTGYLRFDI"
BASE COUNT          662 a         1276 c         1424 g          719 t
ORIGIN      
        1 gtacgaattg gccgatcgcc cggatgacgc ggtcgttgca gctgagcgcg cggtctctac
       61 cgactcgatg tccgccgccg ccgccgcgga gctcggcgac gcgttgtact acgcgcggcg
      121 ttacgacgac gcgctgtcgc agttgatgaa ggtcgtggcc cttcggccgc cacttcgacg
      181 cacggcgttc tatctggacg aggtgtacct ggtcaagcat cgatggaaag aggcgatcga
      241 cgtgatgcgg ccggacacgt cggaccgaaa cagccggggc ctcatcggtt atgcgctggc
      301 gcgatccgga cgtgagcggg acgcgaggcg actgctgcag cggatgctcg ccacgaagtc
      361 gacgtcggcg gcggccgtcg cggaggtgta catcgggctt cgacggtacg acagcgcctt
      421 cgtgtggctg gatcggtcgt tcgatgacca ttcgctgcat ccgatgatca tggggccgct
      481 gttcgacgac gtgcatgctc gccccgagtt caggcgcgtg aggaagcggt tggggctgcc
      541 gcccgggtag agtaatcgca ttgtggacgc atcgcgatcg ttcctaacgc ggtcaatcga
      601 cccgcgatgg tcgctcgcca ctccctcccc atccacacgg agtctcagtt ggtcacgacg
      661 atcgtcccga cgaagttggg atagagcgac ggcggatgaa acgtgcagtg gtacgggaag
      721 ctgcccgttt gattgaacgt gtgctggaac gtcgcaccgg tgggcatcag gccgccggaa
      781 tcccaaatgc ccgtgtcgct ggtcgtgctg tgctccgagg gcccgttatt catccacgtt
      841 accgtcgtgc cgactttgac cgtgtcgacg gcgggcgaaa aactaaaatc ctgaatcgtg
      901 atgttggcgc cgcccatcgg cgtcggcggc ggattcttgg tagtgccggt gatgctcgag
      961 ttgctgccgc cgcaggcggc ggccgcggca atgatgccga tcatgacgaa ttggttcggg
     1021 cgcatgcgca tctccgcgta cgagggtggg gagcacacgt gggcgcaaca atcgttcttg
     1081 gtcgagccgc tcgggcccgc acccgccgca gcgcggtgaa cggcacgcct gatagggcga
     1141 aggcgctgca cggcatgatc agcaacgcgc cattttgtcc gttgccaagc cacacagccg
     1201 gacgcgtccg tctttcgaca aaggaacctt cgcggacacg tgtaagggac gtcgccgacg
     1261 aactttgttg ctcgacgaac gtgtaggagc gaccgacgaa tccgttaggc gacaacgaag
     1321 cgatcgacgc actctgagcg acgcgcgacg cggcgtcgcg tgctcgcgca tcggggttcc
     1381 ccggacagac tgtccggcag agcacgacag cgcgcacggg gcggtgtgtg ctagcgtgca
     1441 ttcgatgtca cgccgggtca ctcttcgagg gggggacatg cgctggtcct ggaagctcgg
     1501 gcgggttgcg gggatcgagc tccgcgttca cgccacgttc ctgctgctgc tcgcctgggc
     1561 ggcgctcacg tactaccgac tgggcggaac gacgggggcg gccgtgcgcg gcgtggtgtt
     1621 cacgcttgcg ttgttcgcgt cggtcgtcct ccacgaactg gggcacgcgg ttgctgcacg
     1681 gcgtttcggc gtgccgacgc gggacattac gctgcttccc atcggcggcg tggcgcggct
     1741 cgcatacatc ccgcgacagc ccaggcaaga gctggtcatc gcgctcgccg ggccgctcgt
     1801 gacgctgctc atcgtgctcg cgctgtacgg catcgggctc ggcttctcgc tgcctaacgg
     1861 gaccatcgat gcggcgttgg gcgcgcgagg cggattcgtg ggacaactca tgtgggcgaa
     1921 cgtgttcctg ctcgtgttca acctgctgcc ggcgttcccg atggacggag gacgcgtgct
     1981 gcgggctgcc ctcgcgctgc acggcgacta cgcgaaggcg acgaacgcag cagcgcgcgc
     2041 cggccgtgcg ttcgcgctcg tcttcggatt ggtcggactg ctctacaacc cgttcctcgt
     2101 cctcatcgcg ctcttcatct ggctcggcgc cgccggagaa gccgccgacg agagactgca
     2161 ttcgtcactc gacggcgtac cggtggagca attgatgatc cgcgatgtgc aaacgctcac
     2221 gccgcgcgac accctcggcg tggccctccg acacgtcctc gccggcttcc agcatgactt
     2281 cccggtgatc gacaacggaa atctcgtcgg gattctcacc agagacgcgc tcgtcaaggc
     2341 gctcgcacgt cacggcgaga atgttctcgt cgacgaggtc atggaccgcg gcttccgaac
     2401 ggccgcaccg ggtgagttcg tcgaaggcgc gctggcgcga ctccgcgaca gtgactgtcg
     2461 gacgatcccg gtgatggcgg atcatcgcct ccaaggcgtg ctcaccctgg acaacatcgg
     2521 ggagttcgtg atgatcaaca gcgcgctgca tggcgcgatg taaaggatgc gatccaccgg
     2581 cgcaccgatg cgatgatcac ggctgcgatg aagatgccgg ccgcgagcag gccggcggaa
     2641 tgatcagcag cgatcccatg aggagcacgc agaggtagcg cagcccgagt gcgaccgtga
     2701 gactcagcga aattccggtg ctcccgcgtt aggcagagga cgtgcgtcgc gtaccggtac
     2761 accacgctca ggtcgtgcga gatgagccgc accgtgaccc cttcgtcgcg gcggagccga
     2821 tcgagcgtcg tgttgagacg ttcctggccc ggttggtcca cctctgccgt cagctcatcc
     2881 ggcaggagca cgttgggcct gagcggacgt cagggcttcc tttacccgat gtggtgccaa
     2941 caccgactgc cgcggcgccg ggcccgtggc acgttgttca tcgtccttca cggttggtga
     3001 accatgcacg caccgttcgt ggaccggcgc gaggccggtc gggcattggc gggaaagctc
     3061 cgggagtacg tcgaccagcc gaacgtgctc gtcctcggcc tgccgcgggg cggcgtgccg
     3121 gtggcgtacg aggtcgcggt cgcgttaggc gcgccgctgg acgtgttcgt ggtgcggaag
     3181 ctgggtgtgc ccggccacga ggagatggcg ttcggcgcca tcgcgagcgg aggggtgcgg
     3241 gtgctcgacc cggtcgtcat cgcccagctc cggctttcgg acgcggcgat cgacgcggtg
     3301 acgcgcgagg agcgcatgga gctcgagcag cgcgaggagc tctatcggcc cgaacgccag
     3361 ttcagcagcg tcgcaggtcg aacggtgatt ctggtcgacg atggcgttgc gaccggcgcg
     3421 agcatgtatg ccgccgtccg tgcgctgcgc caggagcggc cggcgaagat cgtcgtcgcg
     3481 acgcccgtgg cctcggccga cgcgattcgc gcgctggcca acgttgccga cgagtgcgtg
     3541 tgcgtcatgc ggccggtgat cttccaagcc gtggggctgt ggtatctgca cttcgatcag
     3601 acgtcgaacg acgaggtgcg acggctgctg cgcgaggcgc gtgatcgtcc gataggcctg
     3661 accgcggcgg gcagcgcgcc tcccggtgcc gatgcctaac aatgacgccg gctcggcggt
     3721 tcgcacactg cagctcacgc ggtcgccgac agccagtcag cctggatcgc gcgagagtac
     3781 acgaccatac aacgccggca ctgagaccaa gcaggaggac gtcatgacaa cgcaacaaga
     3841 aaccattgga ctccggatac ccgcgcgcgg cgtgacgttg gacggagatc tgatcgctcc
     3901 ggagatgccg gcgggcatca tcgtttttgc acacggcagc ggcagcagcc gcctgagtcc
     3961 gcggaaccgg caagtggcgg cggctctgcg cgcgtccaat ttcgcgacgc tgttgttcga
     4021 cttgctgacg ccggcggaag aagcgatgga tgtggatacg gggtatcttc gtttcgacat
     4081 c
//
LOCUS       DASUCJ010000688         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_954771, whole genome shotgun sequence.
ACCESSION   DASUCJ010000688 DASUCJ010000000
VERSION     DASUCJ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954771"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFU90_08210"
     CDS             <1..703
                     /locus_tag="VFU90_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479799.1"
                     /translation="PQIGVCFDNKTLGDELDAAGLSWRYYTSALSPFSDGTLWNAYQA
                     ISHIYNGPDWKKDVKSPQTRIFDDVKNGKLPAVAWVTPTCRNSDHAACESNHGPQWVT
                     SLVNAIGQSPYWNSTAIFIMWDDYGGWYDHVPPPLEDFDGLGVRVPLLIVSPYAKTGY
                     VSHVQYEHGSILKFIEDQFGLPRLAATDSRANSPAQDCFNFNQSPRRFTPLPTALGPR
                     YFENEAPDTRPVDTY"
     gene            703..>1430
                     /locus_tag="VFU90_08215"
     CDS             703..>1430
                     /locus_tag="VFU90_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479800.1"
                     /translation="MLAHRIIAIAALAALTACSGGAAAGTALPPAGATALVDGRRSSG
                     KITHVVVIVQENRSVDNLFQGFPGADTQSYGYNSKGQKVTLQPIGLEMNWDIDHSVKA
                     FFAACDGTGSLPGTNCKMDGFDKEALTCSRGIHPYCPPADSQYGYVPQSETKPYFAIG
                     KQYVLADHLFSSNIDGSSYTSHQYLIAGQADSAVDFPSAPIWGCDGGKGDTIPTITQS
                     RTYGPSLRVCFNYQTLGDEMDAAG"
BASE COUNT          281 a          451 c          436 g          262 t
ORIGIN      
        1 cccgcagatc ggcgtgtgct tcgataataa gacgctcggc gacgaactcg acgcggccgg
       61 gctgtcgtgg cgctactaca ccagtgcgct cagcccgttc agcgacggca cgctttggaa
      121 cgcttatcag gcgatctcgc acatctacaa cgggccggat tggaaaaagg acgtcaagag
      181 cccgcagacg cgcatcttcg acgacgtcaa gaacggtaaa ctgccggcgg tagcgtgggt
      241 gacgccgacc tgccgcaact ccgatcacgc ggcttgcgag tccaatcacg ggccgcagtg
      301 ggtgacgtcg ctcgtcaacg ctatcggcca atcgccgtac tggaattcga ccgcgatctt
      361 cattatgtgg gacgactacg gcggatggta cgatcacgtc ccaccgccgc tcgaggattt
      421 cgacgggctc ggcgtgcgcg tgccgttgct gatcgtatcg ccgtatgcca agacggggta
      481 cgtgagccac gtgcagtacg aacacggcag cattttaaaa ttcatcgaag atcagttcgg
      541 tcttccgcgg ctggccgcga ccgattcgcg cgccaactcg cccgcccagg attgcttcaa
      601 cttcaaccag tcgccccggc gcttcactcc gctcccaacc gcgctgggtc cgcgctactt
      661 cgagaacgag gcgcccgata cgcgcccggt ggacacctac taatgcttgc acatcgtatc
      721 attgccatcg cggcgcttgc cgcgcttacc gcgtgcagcg gcggcgccgc cgccggcacg
      781 gccctcccgc ccgcaggtgc gaccgcgctg gtcgacggac gccgttcgag cggcaagatc
      841 acgcacgtgg tcgtcatcgt tcaagaaaat cgcagcgtcg acaatttgtt ccagggattt
      901 cccggcgcgg atacccagtc gtacggttat aacagcaagg gccagaaggt cacgcttcag
      961 ccgatcggcc tggagatgaa ttgggacatc gatcactcgg tgaaagcgtt tttcgcagcc
     1021 tgcgacggga cggggagtct tccaggaacg aactgcaaga tggacggctt cgacaaggag
     1081 gcgttgacct gcagccgcgg catccatccc tactgcccgc cggcggattc gcagtacggg
     1141 tacgtaccgc agagcgaaac gaaaccgtac ttcgcgatcg gcaaacagta cgtgctggcg
     1201 gatcacctct tttcgtcgaa tatcgacggc agcagttaca cgtcgcatca gtatctgatc
     1261 gcgggtcaag cggattcggc ggtcgatttc ccctcggcgc cgatttgggg ctgcgacggc
     1321 gggaagggcg atacgattcc gacgatcacc cagtcgcgca cctatgggcc gtcgctgcgg
     1381 gtctgcttca attaccagac gctgggcgac gaaatggatg cggccggcat
//
LOCUS       DASUCJ010000689         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_343760, whole genome shotgun sequence.
ACCESSION   DASUCJ010000689 DASUCJ010000000
VERSION     DASUCJ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343760"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFU90_08220"
     CDS             <1..294
                     /locus_tag="VFU90_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479801.1"
                     /translation="FVPSVHYAGNQTSSVVVAGSQLVPETVTVQGATTMLAQVAAVHL
                     DVALPAEPKAIDEMVRPVPVNTLGNEIAGLEVSPDLVRVQIRFVAGTGETPKP"
     gene            291..1646
                     /gene="glmM"
                     /locus_tag="VFU90_08225"
     CDS             291..1646
                     /gene="glmM"
                     /locus_tag="VFU90_08225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5479802.1"
                     /translation="MNRLFGTDGVRGVANGDLTPDLAFRIGRAGASVLAGSSDRHRPF
                     VVGRDTRLSGTMLEAAIVAGVTSVGRDVALLGIVPTPAVACVTRREGAAAGIMISASH
                     NPVADNGVKFFGPDGFKLSDEIEREIESLLDSTDLPRPTGTGVGIASVRHNLGRHYYR
                     ELYESRADLHGLHAIVDAAFGAAFTVAPYALRRLGATVTELNCRNDGSRINVGCGATD
                     LGPLQAAVRARIEAGEEHVVGVAFDGDADRAMFVDETGSVVSGDRVMYAIGSDLHARG
                     ELAGDAVVGTVMSNVGFERALQRRGIALIRAPVGDRYVLERMLDGGYVLGGEQSGHVI
                     DLRRNTTGDGPMTAMTLFGIVTATRTRLHDLVSDVVEAPQVLLNVATPHKDAAASTGV
                     REAIAAGEAELAGSGRLFVRPSGTEALIRVMAEGDDPSLVAEVAARVVAAIEKEIGNR
                     P"
     regulatory      1698..1834
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1821..2423
                     /locus_tag="VFU90_08230"
     CDS             1821..2423
                     /locus_tag="VFU90_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU5479803.1"
                     /translation="MEEECIFLRHALAVGALVFSIAGSCAVASAAVRPTPSNAAALSP
                     DVAEWTSHLVINTPARHQAGIGRFAGGILARTSKLAQLLARSALRFLGTPYVFGGTSS
                     SGFDCSGFVQHVYGMAGISLPRTADAQYDYGRHAVGGPRVGDLVFFETYTEGVSHVGI
                     YLGKGEFVHASSSHGVMVSNLSDSYWRARYLGAKRLIAAH"
     gene            2513..2584
                     /locus_tag="VFU90_08235"
     tRNA            2513..2584
                     /locus_tag="VFU90_08235"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2545..2547,aa:Gln,seq:ttg)
BASE COUNT          438 a          855 c          901 g          467 t
ORIGIN      
        1 ttcgttccca gcgttcacta cgccggcaat caaaccagtt cggtcgtcgt agcgggctcg
       61 cagctcgttc ccgagacggt tacggtgcag ggggcgacga cgatgctcgc gcaagtcgcg
      121 gcggtgcatc tcgacgtagc cttgccggcc gaaccgaaag ccatcgacga aatggtccgc
      181 cccgtgccgg tgaacacgct cgggaacgag attgcgggct tggaagtgtc tccggatctc
      241 gtgcgcgtgc agattcgttt cgtggcgggc accggcgaga cgcccaaacc gtgaaccggc
      301 tgttcggcac cgacggggta cgcggcgtcg ccaacggcga tctgactccg gacctggcgt
      361 ttcgcatcgg gcgcgccgga gccagcgtct tggccggttc gagcgatcgg caccgcccgt
      421 tcgtggtggg acgcgatacg cgtctctcgg gaacgatgct cgaggcggca atcgtcgcag
      481 gcgttacttc ggtcgggcgc gacgtcgcgc tgctgggcat cgttccgacg cctgcggtgg
      541 cgtgcgtcac gcgccgcgag ggtgcggctg ccggcatcat gatcagcgcg tcccacaacc
      601 cggtcgccga caacggcgtg aaattcttcg gtcccgatgg tttcaagctg tccgacgaaa
      661 tcgaacgcga gatcgaatcg ttgctggatt cgaccgacct tccgcggccc actggaaccg
      721 gggtcgggat cgcgagcgtg cggcacaacc ttggacgtca ttactaccgc gaactctacg
      781 agagccgcgc cgacctgcac ggactgcacg cgatcgtcga cgcggccttc ggcgcggcgt
      841 ttacggtcgc cccatacgcg cttcgaagac tcggcgccac ggtaacggag ctgaattgcc
      901 gcaacgacgg gtcgcggatc aacgtaggct gcggtgcgac ggacctcgga ccgctgcaag
      961 cggccgtgcg cgcgcggatc gaggccggcg aggagcacgt cgtcggcgtg gcgttcgacg
     1021 gagacgccga ccgcgcgatg ttcgtggacg aaaccgggag cgtcgtcagc ggcgaccggg
     1081 tgatgtatgc gatcggcagc gatttgcacg ctcgcggcga actcgccggc gacgcagtcg
     1141 ttgggaccgt catgagcaac gtcgggttcg agcgtgcgct gcagcgtcgc ggcatcgcgc
     1201 tgattcgagc accggttggc gaccgttacg tgctggagcg gatgctcgac ggcggctacg
     1261 tactcggcgg cgagcagtcg ggtcacgtca tcgatttgcg ccgcaacact accggcgacg
     1321 gcccgatgac ggcgatgacc ttgttcggaa tcgtaacggc gacccgaacc cgtctgcacg
     1381 accttgtgag cgacgtcgtg gaggccccgc aagtgctgct caacgtcgcg acgccgcata
     1441 aggatgccgc cgcctcgacc ggcgtacggg aggcgatcgc ggccggcgag gcggagctcg
     1501 ccggctccgg ccgcctgttc gtgcggccct ccggcaccga ggcgctgatc cgggtcatgg
     1561 ccgaaggcga cgacccgtcg ctcgtggccg aggtggcggc ccgggtagta gctgcgattg
     1621 agaaggagat cgggaaccgg ccgtaaaccc aagccgagtc acccggagtt ctaaccgatt
     1681 ccgggaccca ctacgcctgc acaagatcac tgaatcgggc cgagcccgag cgggggattc
     1741 ccaggtaggg gtgagtgagc cggtccggct ctagggcgac tcttttccgc ccgaacccgt
     1801 caactaacct cgcaagtgcc atggaagagg agtgtatatt cttgcgtcac gcgttagctg
     1861 tcggggcatt ggtcttttcg atcgccggtt cgtgtgcggt cgccagtgcc gcggttcgtc
     1921 caaccccgtc taatgccgca gctctcagtc ccgacgtcgc cgaatggacg tcgcatctgg
     1981 tcatcaacac gcccgcacgg catcaggccg gcatcggccg gtttgccggc ggtattctcg
     2041 cgcgaacttc aaagctcgcg cagctgctgg cgcgcagcgc cttgcggttc ctgggaacgc
     2101 cgtacgtgtt cggcggcacc agctcgtccg ggttcgactg ttccggcttc gtccagcacg
     2161 tctacgggat ggcggggatc agcctgccgc gcaccgcgga cgcgcagtac gactacggca
     2221 ggcatgccgt cggaggtccg cgcgtcggag acctggtgtt cttcgaaacc tataccgagg
     2281 gcgtttcgca cgtcggaatc tacctcggca aaggcgagtt cgttcacgcc agctcgagcc
     2341 acggcgtcat ggtgagcaac ctttcggact cgtactggag ggcgcggtac ctgggggcaa
     2401 agcgcctcat cgccgcgcac tgagttctga aaccgccccc ggaagcggtc ttgcgccgct
     2461 tgacaggggg cggttccgtt ccccgataat ctgccttgcc gtcagccttc gatggggtgt
     2521 agccaagcgg taaggcagcg gactttgact ccgccatgcg ttggttcgaa tccagccacc
     2581 ccagcaggtt cccgagaaac gatgaccggc agcgcgtcaa cattgcacgt caccactggt
     2641 gacgcggttc tctacgtctt c
//
LOCUS       DASUCJ010000690         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_374504, whole genome shotgun sequence.
ACCESSION   DASUCJ010000690 DASUCJ010000000
VERSION     DASUCJ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374504"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFU90_08240"
     CDS             <1..977
                     /locus_tag="VFU90_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479804.1"
                     /translation="IGSTVDPTNGDQNPYGLIYVSTKPFGGGVIQKGDLVICNFNDKA
                     NVQGNGTTMEYMKSVAGSKPTRLLQNAKLKGCASLVINGFGQMYAANSGAKNVLGVSA
                     KAKIFQTLTNKAMVEPWGSGYATGFGYPPGDAIFVSDASNGKIFRINLGTSNGQPTYT
                     AVISGFDVNGGAPGSILGPSGVQYNQKADILYVVDGVDNTVVAFKHAYNTLNAANSVV
                     VQPGGKTFKGPQAKNAALIYAGSPLKGPVSSALLPNGNLVIGNTLNPNGTNLMVEIAS
                     NGKLLATKNVDKGAAGALFGIVAIGSSDATTKIFFNDDNKNNVQVLSE"
     gene            complement(934..>1498)
                     /locus_tag="VFU90_08245"
     CDS             complement(934..>1498)
                     /locus_tag="VFU90_08245"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479805.1"
                     /translation="RIMLHLNETRDPDGFDPPAGFVRTSICAVTGHAPQPGCPAVVEE
                     WVRPHDLASVRRTVPLRLGSEYDTWLALHPQDATAARLRIVFPRNGDVFVANPASTSL
                     QAREQELALRAVASGRAILWSANGKTVPLDSSGTAFLPVRVGTWTIVAEDGRQRDRVT
                     IRVVPRERDARPGFTRTTPARYSCCRR"
BASE COUNT          316 a          468 c          475 g          239 t
ORIGIN      
        1 ttatcggatc gacggtcgat cccaccaacg gcgatcagaa cccgtacggg ctgatctacg
       61 tttctacgaa accgttcggc ggcggagtca tccaaaaggg tgacctcgtc atttgcaact
      121 tcaacgataa agccaacgtg caaggcaacg gcacgacgat ggaatacatg aagtcggtag
      181 ccggctcgaa accgacgcgc ctcttgcaga acgcgaagct caagggctgc gcgtcgctgg
      241 tgatcaacgg cttcggccag atgtacgcgg cgaattcggg cgcgaagaac gtgctgggcg
      301 tttcggcgaa ggccaagatt ttccagaccc ttaccaataa agccatggtc gagccgtggg
      361 gaagcggcta cgcaaccggc ttcggctacc cgccgggaga cgcgatcttc gtctccgacg
      421 cctcgaacgg gaaaattttc cggatcaatc tcggcacgag taacgggcaa ccgacgtaca
      481 cggcggtcat cagcggattc gacgtcaacg gcggcgcacc ggggagtatt ctcggaccgt
      541 cgggcgtcca atacaaccag aaagccgaca tcctctacgt ggtcgacggc gtcgacaaca
      601 ccgtcgttgc cttcaaacac gcctataaca ccttgaatgc ggccaacagc gtcgtcgtcc
      661 agccgggcgg caagacgttc aaggggccgc aagccaagaa tgcggccttg atctacgccg
      721 gcagcccgct caagggcccg gttagctccg cgctgcttcc gaacggcaac ctcgtgatcg
      781 gcaacacact caatccaaac ggaacgaacc tgatggtaga gatcgcgagc aacggcaagc
      841 tgttggcgac gaagaacgtc gacaaaggcg cggccggggc gctgttcggc atcgtggcga
      901 ttggttcgtc ggatgcgacg accaagatct tcttcaacga cgacaacaag aataacgtgc
      961 aggtgttgtc cgagtaaatc ccgggcgcgc gtcgcgctcg cgcggcacga cgcgtatcgt
     1021 tacgcggtcg cgctgacggc cgtcctcggc aacgatcgtc cacgtcccca cacggacggg
     1081 cagaaaggcg gtcccgctcg aatcgagcgg gaccgttttt ccgttcgcgc tccagagaat
     1141 cgcgcggccc gaggcgaccg cacgcagcgc cagctcttgt tcgcgcgcct gcagcgacgt
     1201 ggaagccgga ttggcgacga atacgtcgcc gttgcgcgga aagacgatgc gcaggcgcgc
     1261 cgcggtggcg tcctgtgggt gcagcgcgag ccaggtgtcg tactccgaac cgagccgcag
     1321 cgggaccgtc cggcgcaccg atgcaagatc gtgcgggcgc acccattctt cgacgacggc
     1381 cggacagccg ggctgcggtg cgtggccggt gaccgcgcaa atactcgtgc gcacgaaccc
     1441 ggccggcgga tcgaagccgt ccggatcgcg cgtctcgttg agatgcagca tgatccgg
//
LOCUS       DASUCJ010000691         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_756212, whole genome shotgun sequence.
ACCESSION   DASUCJ010000691 DASUCJ010000000
VERSION     DASUCJ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756212"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1256)
                     /gene="dnaJ"
                     /locus_tag="VFU90_08250"
     CDS             complement(150..1256)
                     /gene="dnaJ"
                     /locus_tag="VFU90_08250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5479806.1"
                     /translation="MPTKDYYEVLGVARDASGDEIKAAYRKLARRHHPDVSDDKSDAE
                     LRFKAINEAYEVLSDPNKRAQYDRFGSTGNGAGDFGFGGGGFGDIFDMFFGNVRGAQA
                     RRAGPQRGSDLRYDVEIALEEAFSGTTKEIAFSHLARCDTCNGSGARPGTLATPCTRC
                     GGSGIERGVRNTPLGQFVTQTTCARCGGEGHVVEHPCEACGGRGARDAERRLTVTIPA
                     GVDDGSRIRIAGNGEAGTHGGPPGDLYVYVGIAAHPYFKRSGADTYADVPVSFPQAAL
                     GGPIAARTLEGPVEMNLPPGTQSGTMLRLRGKGMPQVRGSHRGDHHVTVHVVVPAKLN
                     KRQRELLEEYARAGGDAVEERTFFERVKDAFRPE"
     gene            complement(1274..2323)
                     /gene="hrcA"
                     /locus_tag="VFU90_08255"
     CDS             complement(1274..2323)
                     /gene="hrcA"
                     /locus_tag="VFU90_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5479807.1"
                     /translation="MRDEQGGLDKRKAYILATVVYEYIATAEPVGSQTLTQKYQLGVS
                     SATVRNEMAELEAEGYLVQPHTSAGRIPSDAGYRTYVDRLMEPEELGVEERRRIRDEL
                     HDASRELDEIIENTTRLLGRLSNNLAFVTKPHQETQTFKHIQLIWLTPRTGVAIVVTS
                     LGVEAQSLFELAAEMRPDDLTRFSNALNDRFTNYPLRDVLEKEVAKVAGEIGAHEDLR
                     RAVFAALQSARSSGEPNIASAGAQNLLDQPEFHDLRKLRSILRIVEEQKTLYQIVADA
                     MTSEAPSVRIGHELGHEELADLSVVTVPYRFGNAALGMLSILGPRRMPYGRLLALASG
                     TAASLSERLSDVEQH"
     gene            complement(2330..2839)
                     /locus_tag="VFU90_08260"
     CDS             complement(2330..2839)
                     /locus_tag="VFU90_08260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5479808.1"
                     /translation="MFGWGALILAALTATASTGLTGIRYDAPTPDFSIPSGHGARYLS
                     SLRGRVVVIDFWASWCDVCTAEMKYFVRAKQTFGDRLAVVTVSDEPHDVAASYFRLWN
                     IALPVVEDESGAISRVYSVSKIPVTLVLDPSGKVVYVSVGGLSWEELDGAIRQAGLAA
                     STQTSRVLQ"
     gene            2891..>3120
                     /locus_tag="VFU90_08265"
     CDS             2891..>3120
                     /locus_tag="VFU90_08265"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5479809.1"
                     /translation="MSNDAAASIEALSQESRTFPPDAAFARQANAQPGIYDDAERDYL
                     AFWASWARKLDWMKPFTTTLQWNEPYAKWFADG"
BASE COUNT          483 a         1061 c         1016 g          560 t
ORIGIN      
        1 cgccgagtcg acgatttcga tgacgtcgcc gtcgcgcagc cgcaggacgc gcgcgatctt
       61 atgggcgtcg ctcccggcga tcgcaacggt ctcgccggtc gagcgaacgc cttcgacgaa
      121 aaatcgccgg gcgggcatcg ctgttcgcgc tattccggcc ggaacgcgtc cttcacgcgc
      181 tcgaagaacg tgcgttcctc gacggcgtcg ccgcccgcgc gcgcgtattc ttcgaggagt
      241 tcgcgctgcc gtttgttcaa cttcgccggg acgacgacgt gcaccgtcac gtggtgatcg
      301 ccgcgatggc tgccgcgtac ttgcggcatt cccttcccgc gcagacgcag catcgtgccg
      361 ctctgcgttc ccggcggcaa attcatctcg accggccctt ccagcgtgcg cgccgcgatc
      421 ggtccgccga gcgccgcctg cggaaagctc accggaacgt cggcgtacgt gtcggcaccg
      481 ctgcgtttga aatatgggtg cgcggcgatt ccgacgtaca cgtacagatc gcccggcggc
      541 ccgccgtgcg tgcccgcttc gccgttgccg gcgatccgga tgcgcgatcc gtcgtcgacg
      601 ccggccggaa tcgtgacggt gagccgccgc tccgcgtcgc gcgctccgcg gccgccacag
      661 gcttcgcacg gatgctcgac gacgtggcct tcgccgccgc agcgcgcgca cgtcgtttgc
      721 gtgacgaact gtccgagcgg cgtgttgcgc acgccgcgtt cgatgccgct tccgccgcag
      781 cgcgtgcacg gcgtggcgag cgttccgggg cgagcgccgg agccgttaca cgtgtcgcag
      841 cgcgccaaat ggctgaacgc gatctctttc gtggtccccg aaaacgcctc ttcgagcgcg
      901 atctccacat cgtagcgcag gtccgagccg cgctgcggcc cggcccggcg cgcctgcgcg
      961 ccgcgcacgt tgccgaagaa catgtcgaag atgtcgccga agccgccccc tccgaagccg
     1021 aaatcgccgg ctccgttgcc ggtgcttccg aagcggtcgt actgcgcgcg tttgttcggg
     1081 tccgagagca cttcgtaggc ttcgttgatt gccttgaagc gcagctcggc atccgatttg
     1141 tcgtcggaga cgtcgggatg gtggcgccgc gcgagcttgc gatacgcggc tttgatctcg
     1201 tcgccggagg cgtcgcgtgc gacgccgagg acttcgtagt aatccttggt gggcataggc
     1261 tagattgcga gactcagtgc tgctctacgt ccgagaggcg ttcgctcaag ctcgcggccg
     1321 tgcccgacgc gagcgccagc agccggccgt acggcatccg ccgggggccg aggatcgaga
     1381 gcattcccag cgccgcattt ccgaagcggt acggcaccgt aaccaccgac agatcggcga
     1441 gctcttcgtg gccgagctcg tgaccgatcc gcacgctcgg cgcttcgctg gtcatcgcgt
     1501 cggcgacgat ctggtaaagc gtcttctgtt cttcgacgat ccgcagaatc gagcgcagct
     1561 tgcgcaggtc gtggaactcg ggctggtcga gcagattctg cgcgccggcc gacgcgatgt
     1621 tcggctcccc gctcgaacgc gccgactgca gcgcggcgaa caccgcgcgc cgcaaatctt
     1681 cgtgcgcgcc gatctcgccg gccaccttgg cgacttcctt ttcaagcacg tcgcgcagcg
     1741 ggtagttcgt gaagcggtcg ttgagcgcgt tggagaagcg cgttaggtcg tcgggacgca
     1801 tctcggcggc cagctcgaac aggctttgcg cttcgacgcc gagcgacgtg acgacgatcg
     1861 ccaccccggt ccgcggcgtg agccagatca gttgaatgtg cttgaaggtc tgcgtctctt
     1921 ggtgcggctt ggtgacgaac gcgaggttgt tcgaaagccg ccccagcagc cgcgtggtgt
     1981 tttcgatgat ctcgtccagc tcgcggctgg cgtcgtgcag ttcgtcgcga atccggcgcc
     2041 gctcttcgac gcccagctct tcgggctcca tcagccggtc gacgtaggtc cggtaacccg
     2101 cgtccgaggg gatgcgcccg gcggaggtgt gcggctgcac gaggtagccc tccgcctcca
     2161 gctccgccat ctcgttgcgg acggtggccg agctgacccc cagctggtac ttctgcgtga
     2221 gggtttgcga gccgaccggc tcggcggtgg cgatgtactc gtacaccacc gtcgctagaa
     2281 tataggcctt gcgcttgtcg aggccgccct gctcgtccct catagcctcc tactgtagca
     2341 ctcttgaagt ttgagtgcta gccgcgaggc cggcctgccg gatggcgccg tccagctcct
     2401 cccagctcag cccgcccacc gacacgtaga cgaccttgcc gcttgggtcg agtaccaggg
     2461 taaccggaat cttggagacc gagtagacgc gcgagatcgc ccccgactcg tcttcgacca
     2521 ccggcaacgc gatgttccac aggcggaagt aactggccgc gacgtcgtgc ggctcgtcgg
     2581 aaacggtcac caccgcgagc cggtcgccga acgtttgctt tgcccgcacg aagtatttca
     2641 tttcggcggt gcagacgtcg caccacgacg cccaaaaatc gatgacgacg acgcgtccgc
     2701 gcaggctcga cagataccgc gcgccgtgcc cgctgggaat cgagaaatcc ggcgtcggcg
     2761 cgtcgtagcg aatcccggtc aaaccggtgc tggccgtcgc cgtaagtgcc gcgagtatca
     2821 gcgcacccca accgaacacg tacgaccatt tcggcaggcg cgagcagccg gcctcgtcaa
     2881 aacggcggtt atgagcaacg acgcagcagc ctcgatcgaa gcgctttccc aagaatcgcg
     2941 aacgtttccg ccggacgccg cctttgcccg tcaagccaac gcgcagcccg gcatctacga
     3001 cgacgccgag cgcgactatc tggcgttttg ggcgtcgtgg gcgcgcaagc tcgattggat
     3061 gaaaccgttc accaccacgc tgcagtggaa cgagccgtat gcgaagtggt ttgccgacgg
//
LOCUS       DASUCJ010000692         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_22681, whole genome shotgun sequence.
ACCESSION   DASUCJ010000692 DASUCJ010000000
VERSION     DASUCJ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22681"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFU90_08270"
     CDS             <1..460
                     /locus_tag="VFU90_08270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU5479810.1"
                     /translation="WTDLQWIREAWGPAPIVVKGILDADDARRAVDAGADAVIVSNHG
                     GRQLDGVRPALRALPEVANAVGSQVEVLMDGGIRRGSDVVKALCLGARAVLAGRAYAY
                     GLGGAGGAGVARAIEILRSGVMRTLRLLGCHDVAALDRSYVECPAEWLRA"
     gene            515..1147
                     /locus_tag="VFU90_08275"
     CDS             515..1147
                     /locus_tag="VFU90_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479811.1"
                     /translation="MKVLQRQAAPAGRPQPLAFHRGRLWVGSWDTDRLYAIDPQTWKV
                     VDDVAAPGKPYGMAVLGDGIVTVVAIGDDDDRYLSRFVPGRGFDSNPTPCPDFTGSHL
                     AADGETLYLCQQGNRRILVLDEGGGVRREIALTTRCAGVAFESPGKSFIIAADAEFEN
                     LELAAYDLRQNAPEPAPVAAVPFDARGLTFDGTQWWTSHREENEIVSFTP"
     gene            complement(1137..2021)
                     /locus_tag="VFU90_08280"
     CDS             complement(1137..2021)
                     /locus_tag="VFU90_08280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5479812.1"
                     /translation="MIIFKGNNGVRSGWRFLAYVAIVVGLNYLIGLIAEPLFKSLHLS
                     NAWLNVLGPGQLFAGEVLFAVPVIVATFVMARLERRSILSYGFAPSPRSSERFFEGVL
                     LGVIAPGIVGVLMIAFGGMRIHGPGLSGTGWIVYPLGWLGVMLMVGFFEEACFRGYPL
                     YALSRGVGFWPAAIVVTLLFGAAHLTKQGENAVDISSVMLLGLFMCYTLLKTGSLWLA
                     AGFHFAFDFVQFFVIGTRNGSQVPHGTLFNVTFPGPAWVNGGPLGTEASYFVFPVTLA
                     LFAYVAWRFSDRNLIVKA"
     gene            complement(2018..2362)
                     /locus_tag="VFU90_08285"
     CDS             complement(2018..2362)
                     /locus_tag="VFU90_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479813.1"
                     /translation="MVVSAIQPRAWPAAPVAAPGAPPRIVRIWMSSLTVEAGTWLDGA
                     IVASTNVASVEVRTAAFSIDSDHVGPGLFRFHTHVLELPPLGRRHVLGLSIIARNTAG
                     EQAVEYASITIQ"
BASE COUNT          501 a          846 c          881 g          413 t
ORIGIN      
        1 gtggacggat ctccaatgga ttcgcgaggc gtgggggccg gcgccgatcg tcgtcaaggg
       61 gatcttggac gccgacgatg cgcgccgggc ggtcgatgcg ggcgccgatg cggtgatcgt
      121 ctccaatcac ggaggaaggc agctcgacgg cgtgcgtccg gcgctgcgcg cgctgcccga
      181 agtcgcaaac gccgtcggtt cgcaagtcga ggtgctgatg gacggcggca tccggcgcgg
      241 cagcgacgtc gtcaaggcgt tgtgtctcgg agcccgtgcc gtgctggccg gacgcgcgta
      301 cgcctacggc ctcggcggtg ccgggggcgc gggcgtcgcg cgcgcgatcg agattctgcg
      361 ttcgggcgtg atgcgtacgc tgcggctgct gggctgtcac gacgtggccg cgctcgaccg
      421 gtcctacgtc gagtgtcctg cggagtggct gcgcgcctga cgcgtgcgcc gcaaccaccg
      481 ccggctcgcg cgtatcctta aacgtatgca agacgtgaaa gtactacaac ggcaggcggc
      541 accggccggc cggcctcagc cgctggcgtt ccatcgcggc cgcctctggg tcggatcgtg
      601 ggataccgat cgcctctacg cgatcgatcc gcaaacgtgg aaggtggttg acgacgttgc
      661 ggcgccgggg aaaccctacg gcatggccgt cctcggcgac ggtatcgtga ccgtcgtggc
      721 gatcggcgac gacgacgatc ggtacttgtc ccggttcgtg ccgggccgag gcttcgattc
      781 gaatccgacg ccgtgccccg acttcaccgg ctcgcacctg gcggccgacg gcgaaacgct
      841 ctacctatgc cagcagggga accggcggat tctggtcttg gacgaaggcg gcggcgtgcg
      901 tcgcgagatt gcgctgacga cccgctgcgc gggcgtcgct ttcgagtcgc cgggcaaatc
      961 gtttatcatc gccgccgacg cggagttcga aaacctcgag ctggcggctt acgatcttcg
     1021 tcaaaacgca cccgagccgg caccggtggc cgcggttccg ttcgacgcgc gcggcctgac
     1081 gttcgacggc acgcagtggt ggacgagtca ccgcgaagaa aacgagatcg tgtcgtttac
     1141 gccttgacga tgagattgcg gtccgaaaag cgccacgcga cgtaggcgaa cagcgccagc
     1201 gtcaccggaa acacgaagta gctcgcctcg gttcccagcg ggccgccgtt gacccaggcc
     1261 ggtcccggga aggtcacgtt gaaaagcgtg ccgtgcggga cctgcgaccc gttacgcgtg
     1321 ccgatgacga agaactgcac gaagtcgaac gcaaaatgaa aaccggccgc cagccagagc
     1381 gatccggttt tcagcagcgt gtaacacatg aataacccga gcagcatgac gctcgagatg
     1441 tcgacggcgt tttcaccctg cttcgtgaga tgggccgcac cgaagaggag cgtcacgacg
     1501 attgcagccg gccaaaagcc gaccccgcgg ctgagagcgt acagcgggta tccgcgaaaa
     1561 caggcttcct cgaagaaacc caccatcagc atgacgccga gccatcccag cgggtaaacg
     1621 atccagcccg ttccgctcag acccggcccg tgaatgcgca tgccgccaaa ggcgatcatc
     1681 agcacgccga cgatgccggg agcgatcacg ccgagcagca cgccttcgaa gaatcgctcg
     1741 ctggatcgcg gcgacggcgc aaatccgtag gagaggatcg aacggcgttc gagccgcgcc
     1801 atcacgaacg tcgcgacgat caccggcacg gcgaacagca cctcaccggc aaacagctgg
     1861 ccggggccga gcacgttgag ccacgcattc gagaggtgca gcgacttgaa cagaggctcc
     1921 gcgatgaggc cgatcagata gttgagtccg acgacgatcg caacgtaggc gaggaatcgc
     1981 cagcccgacc ggacgccgtt gttccccttg aagatgatca ttgaatcgtt atcgaggcgt
     2041 actcgacggc ttgctcaccg gccgtgttac gcgcgatgat cgacaagccc agcacgtgcc
     2101 gccgcccgag cggcgggagc tcgagaacgt gcgtgtggaa gcggaagaga cccggcccga
     2161 cgtgatcgga gtcgatcgaa aatgccgccg tacgcacttc caccgacgca acgttggtgc
     2221 tcgcgacgat cgcgccgtcg agccacgttc cggcttcgac ggtcaagctc gacatccaga
     2281 ttcgcacgat gcgcggcggc gcgccgggcg ccgcaaccgg tgccgccggc cacgcgcgcg
     2341 gttggattgc cgaaacgacc atattgctcg gagttggaat cggcttggat gcgcatgcag
     2401 cgaacgtcat cgaaagcacc cccgacatta gcgcgggaag gggtttgacg gcgagtcgaa
     2461 acatgaaatt acttcatccc cttcctcgcg aggacaagcc gagcctttcg tgaaccagcg
     2521 cgctctcgtc gacgaccgat ccggcgccag ccggctcgat tggctgatca ccttcgccgg
     2581 ggtctggatc atgaccgggc tgttcatcga cgcgcacgag cacatcttcg agaccatcga
     2641 c
//
LOCUS       DASUCJ010000693         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_145263, whole genome shotgun sequence.
ACCESSION   DASUCJ010000693 DASUCJ010000000
VERSION     DASUCJ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145263"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>1112
                     /locus_tag="VFU90_08290"
     CDS             186..>1112
                     /locus_tag="VFU90_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5479814.1"
                     /translation="MPRLVVVSNRVGIPDGGARAGGLEVAIRPALKRRGGIWFGWSGK
                     ISDGATGPAKTTVQDNVSYVTVDMLRDDHEEFYNGFANRVLWPILHYRLDLAEFSRRD
                     LGGYFRVNEYFARNLETFLRPDDVVWVHDYHLIPLAKALRERGHRNRIGFFLHIPCPP
                     PEILTALPNHERLMPALGAYDLIGLQTETDASNICRYLERECRVRRTEDGVFDFDGRS
                     VRVGVYPVGVETEPFARLARRAIDTAFVREVLESLSGRAMLIGVDRLDYSKGIPERLN
                     AFERFLSNFPDWRNKVTYLQITPRSRTGIAEYA"
BASE COUNT          186 a          363 c          357 g          206 t
ORIGIN      
        1 ctgagaattg gtcgaaccgc gcgcgcatgc gccgaacggc ggtgcacttg taccgtcaaa
       61 aagcgcgaca ccgccgcgca tgctccctcc cgggccccct gaaccagagc agctttcagg
      121 cgttgttcca tcaggccgat ctggcgcatg cgggcttcgg cggccgccgg gggaggatcg
      181 gtaatttgcc aagattggtc gtcgtatcga accgcgtcgg aatcccggac ggcggcgcgc
      241 gtgccggcgg cctcgaggtg gcgatccgcc ccgcgctcaa gcggcgcggc ggtatctggt
      301 tcggctggag cggcaagatt tcggacgggg ccacggggcc ggcgaagacg acggtgcagg
      361 ataacgtctc ctacgtgacc gtcgacatgc tccgcgacga tcacgaggag ttctacaacg
      421 gctttgccaa ccgcgtgctg tggccgatcc tgcattaccg gctcgacctc gccgaatttt
      481 cacgccgcga tctcggcggc tattttcgcg tcaacgagta tttcgcgcgt aatctggaga
      541 cgttcctgcg gcccgacgac gtcgtgtggg tgcacgacta tcatctgatc ccgctcgcca
      601 aggcgctgcg cgagcgcggg catcgcaaca ggatcggttt cttcctccat attccgtgcc
      661 cgccgccgga gatcctgacg gcgctgccga accacgaacg gctgatgccc gccctcggcg
      721 cctacgattt gatcggcctt cagaccgaga ccgacgccag caacatctgc cgttatctcg
      781 aacgggaatg ccgcgtgcgg cgcacggagg atggcgtttt cgatttcgac ggacgcagcg
      841 tacgcgtcgg cgtctatccg gtcggtgtcg agacggagcc gttcgcgcgg ctggcgcggc
      901 gcgccatcga cacggccttc gttcgcgaag tgctcgagag cctgtcggga cgcgccatgc
      961 ttatcggcgt cgaccggctc gattactcga agggcatccc ggagcggctc aatgctttcg
     1021 agcgctttct ttcgaatttt ccggactggc gcaacaaggt cacctatctg caaatcaccc
     1081 cgcgcagcag aacggggatc gccgagtacg cc
//
LOCUS       DASUCJ010000694         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_99359, whole genome shotgun sequence.
ACCESSION   DASUCJ010000694 DASUCJ010000000
VERSION     DASUCJ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99359"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1666
                     /locus_tag="VFU90_08295"
     CDS             <1..>1666
                     /locus_tag="VFU90_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479815.1"
                     /translation="QATLQRVWLDVNATGNASWPVYAAQIGTFPATLYGSDVRRRDGM
                     RTSLAVEGATPEEHVVASGSGSGSARMAVPPGYGAGAVTVTLSPSVLAQLVQSLRLLQ
                     VYPYYCTEQTLSAALPALYVERALRRANATSFYDLRTRDVIVKALARLYQLRHLDGSY
                     GWWEHDAAHPFMTAYALYALAEFGKAGYFVGTGPTVDSLVEQLQASNDDTLRFWGGRQ
                     PGSEWNTRAFMLFALADAAPERVDPTMLADARAHAPAMNAYALAVLGLAERLVGDDAS
                     ARELLRMLDARVVDNGRLAYWPGQTWDYAWEDDPIETTAYALRLQTALEPESPLIPRV
                     VAFLRGQMRGGWAYTTKDTAATIYALSDALPADPNELNPNETVRVTVDGRTVRSLHVT
                     QPILDAADAEVVIPAGEVRNGSVVGVVRSGGGALYWATDFTRYVPPAARAVVDKQTPL
                     LERLFPPQPEVTISRRYVPEQAGPWRVGERIAVEITVTSRCDLQYVAIDDPFPAGAEH
                     PIDQGTAAEYNWSGLQFLDDRATFFADRLDAGETLVLRYALDLTTPG"
BASE COUNT          272 a          498 c          608 g          288 t
ORIGIN      
        1 caggcgacgc tgcagcgtgt ttggctcgat gtgaatgcga ccggcaacgc ttcgtggccg
       61 gtatatgcgg cgcagattgg aacgtttccg gcgacgttgt acggctcgga cgttcggcgc
      121 cgggatggga tgcggaccag cttggcggtc gagggcgcga ctccggagga gcacgtcgtc
      181 gcgagcggct ccggcagcgg cagcgcacgg atggcggtcc cgccgggata tggcgcgggc
      241 gcagtaacgg tgacgctgtc gcccagcgtg ctcgcgcaac tggtccagag cctgcggttg
      301 ctgcaggtgt acccgtacta ctgcaccgag cagacgctgt cggcggcgct cccggcgctg
      361 tacgtggagc gggcgttgcg ccgcgcgaat gcgaccagtt tctacgatct gcggacgcgc
      421 gacgtcatcg tgaaggcttt ggcgcggctg taccaactgc ggcatctcga cggatcgtac
      481 ggctggtggg aacacgatgc ggcgcatccg ttcatgaccg catacgcact ctacgcgctg
      541 gcggaattcg ggaaggccgg ctacttcgtc ggcaccgggc cgacggtcga tagtttggtc
      601 gaacagctgc aggcatcgaa cgacgatacg ctgcggtttt ggggcgggcg gcagccgggc
      661 agcgagtgga acacgcgcgc atttatgctc ttcgcgctgg cggatgcggc accggagcgg
      721 gtcgacccga caatgctggc cgacgcgcgg gcacacgctc cggcgatgaa tgcctacgcg
      781 ctcgcggtgc tcggattggc ggaacgtttg gtgggtgacg atgcttccgc gcgcgagctg
      841 ttacggatgc tcgatgcgcg cgtcgtcgat aacgggaggc tcgcgtactg gccggggcaa
      901 acctgggatt atgcgtggga agacgatccg atcgagacga cggcatacgc gctgcgtctg
      961 cagacggcac tggagccgga gagtccgctg attccacgcg tggtcgcgtt tctgcgcggg
     1021 cagatgcgcg gcgggtgggc atacacgacc aaggacaccg cggcgacgat ttacgcgcta
     1081 agcgacgcac tgccggcgga tccgaacgag ctgaatccga acgaaacggt gcgcgtgacg
     1141 gtcgacggcc ggacggtacg ttcgcttcac gtgacgcagc cgatcctcga tgccgccgat
     1201 gccgaagtcg taattcctgc gggtgaagtt cgcaacggct cggtcgtcgg cgtcgtccgg
     1261 agcggcggcg gcgcgttgta ttgggcgacg gatttcacgc gatacgttcc gccggcagcg
     1321 cgagcggtag tcgacaagca gacgccgctg ctcgagcggc tgtttccccc gcagccggag
     1381 gtgacgattt cacgccgcta cgttcccgag caggcggggc cgtggcgcgt aggcgagcgg
     1441 atcgccgtgg agattacggt aacgagccgg tgcgatcttc agtacgtcgc gatcgacgat
     1501 ccattcccgg ccggcgcaga gcatccgatc gatcagggca cggcggccga gtacaactgg
     1561 agcgggctgc agtttctcga cgatcgcgcc acgtttttcg ccgatcggct cgatgccggc
     1621 gaaactctgg tgttgcgcta cgcgctcgat ctgacgaccc caggtg
//
LOCUS       DASUCJ010000695         1359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_282548, whole genome shotgun sequence.
ACCESSION   DASUCJ010000695 DASUCJ010000000
VERSION     DASUCJ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282548"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFU90_08300"
     CDS             <1..819
                     /locus_tag="VFU90_08300"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU5479816.1"
                     /translation="PRKAESHMETPLKADIGVFGGSGFYSLIEGAREAWVETPYGPPS
                     DRIALGEIAGKRVAFLPRHGKDHRFPPQAINYRANAYAMKLLGVRWLLGPAAAGSLKE
                     SVAPGSMVVCDQMVDRTKGRIDTFYDGPRTTHVSFADPYCPTLRPLAVDALRSLGIET
                     QDGGTVVVIQGPRFSTRAESKWFQSQGWEVVNMTQYPECYLARELEMCYVNISLITDY
                     DVGLEGMEPVSKDAVIEVFNRNNARLNAAIGEIVRRIDVDAGCSCRHALDEARF"
     gene            819..>1359
                     /locus_tag="VFU90_08305"
     CDS             819..>1359
                     /locus_tag="VFU90_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479817.1"
                     /translation="MIRNLNLATYARAVPLLLRHPSILAVPLLAAVVDVLIAQLTPLM
                     TDALGGAGSGLFALLVQLVYLWAFGVAIVQASNIWRGRRGNFDEAWEESRAKFGGIAL
                     AAIGCQFVIWAASYVGSFFGSLSGYLSLALGAVAAFFLIYTIPAAAIGGMPGSLALSA
                     SIRGVRANVVPSLVLAIVFV"
BASE COUNT          215 a          430 c          448 g          266 t
ORIGIN      
        1 cctcgaaaag cggaatcgca catggaaaca cctttgaagg ccgatatcgg cgtattcggc
       61 ggctccggat tttattcgct gatcgagggt gcgcgcgagg cgtgggtcga gacgccgtac
      121 gggccgccca gcgatcgcat cgcgctcggc gaaatcgcgg ggaagcgggt ggcgttcctc
      181 ccacggcacg gaaaggatca ccggtttcca cctcaggcga ttaactatcg cgccaacgct
      241 tacgcgatga agctgctcgg cgtccgctgg ctgctcggcc ccgccgctgc gggctcactc
      301 aaggaatccg tcgcgcccgg ctcgatggtg gtttgcgatc aaatggtgga tcgcacgaag
      361 ggccggatcg atacgttcta cgacggtccg cgcacgacgc acgtcagctt tgccgatccg
      421 tactgcccga cgctgcgccc gctcgcggtc gacgcgcttc gttcgctcgg catcgaaacg
      481 caggacggcg gtacggtggt ggtgattcaa gggccgcgtt tttccacgcg ggccgaatcg
      541 aagtggtttc agagccaagg ttgggaagtc gtcaacatga cgcagtatcc cgagtgctac
      601 ctcgcgcgcg agctggaaat gtgctacgtc aacatctcgc tcatcaccga ctacgacgtc
      661 ggcctcgagg gaatggagcc ggtgtccaag gacgcggtca tcgaggtgtt caaccgcaac
      721 aacgcgcggc tcaacgcggc gatcggcgag atcgttcgcc gcatcgacgt cgacgccggc
      781 tgttcgtgcc ggcacgcgct cgacgaggct cgcttctagt gattcgcaat ttaaatctcg
      841 caacgtacgc gcgcgccgtt ccgctgctgc tgcggcatcc gtcgatcttg gccgtgccgc
      901 tgctggcggc agtcgtcgac gtcctgatcg cgcagctgac gccgctcatg accgacgcac
      961 tcggcggtgc cggcagcggg ctgttcgcgc tgctcgtaca attagtttat ctgtgggcgt
     1021 tcggcgtcgc gatcgttcag gccagcaaca tctggcgcgg acgccgcgga aacttcgacg
     1081 aagcgtggga ggaatcgcga gcgaagttcg gcgggatcgc gctcgcggcg atcggatgtc
     1141 agttcgtcat ctgggcggcg tcgtacgtcg gatcgttctt cggatcgctg agcggctacc
     1201 tctcgctcgc cctcggggcg gtcgccgcgt ttttcctgat ctacacgatt ccggcggccg
     1261 cgatcggcgg catgccggga tcgctcgcgc tctcggcgtc gatccgcggc gtgcgcgcga
     1321 acgtcgtccc gtcgcttgtc ctcgcgatcg tcttcgtag
//
LOCUS       DASUCJ010000696         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_175437, whole genome shotgun sequence.
ACCESSION   DASUCJ010000696 DASUCJ010000000
VERSION     DASUCJ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175437"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFU90_08310"
     CDS             complement(<1..393)
                     /locus_tag="VFU90_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5479818.1"
                     /translation="MRLLLVEDDPILGNGVEVGLKEAGFTVDWARDGRSAQLALETTA
                     YELVVLDLGLPRVTGMELLRALRARGSDLPVLVLTARDTVRDRVAGLEAGADDYLIKP
                     FDLAELVARIRALLRRAHGRSVDAIRYRD"
     gene            573..860
                     /locus_tag="VFU90_08315"
     CDS             573..860
                     /locus_tag="VFU90_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU5479819.1"
                     /translation="MKAYASKAAAALALSVLSVGALASTYNGECTDQPKSKWMTTADV
                     KARFEAQGYNVGRVKTGGSCYEVYAKDKNGTKVELFVNPVDARVVGQAGTQ"
     gene            857..>1087
                     /locus_tag="VFU90_08320"
     CDS             857..>1087
                     /locus_tag="VFU90_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479820.1"
                     /translation="MKPAAFSVAATRAPARVETVRVWDPLVRVFHWSLVAAFFGAYAL
                     GDDGGALHRALGYTALGLVGFRLAWGIVGGRYA"
BASE COUNT          181 a          367 c          363 g          176 t
ORIGIN      
        1 gtcgcggtag cggatcgcat cgacgctgcg cccgtgcgcg cgccgcagca gcgcgcggat
       61 gcgcgcgacg agctcggcga ggtcgaacgg cttgatcagg taatcgtcgg ctccggcttc
      121 gaggccggcc acgcgatcgc gtacggtgtc gcgcgccgtc agcacgagca ccgggaggtc
      181 gcttccgcgc gcacgaagcg cacgcaacag ctccatgccg gtgacgcggg gcaagccgag
      241 atcgagcacg accagttcat aggccgtggt ctcgagcgcg agctgcgcgc tgcgtccgtc
      301 gcgcgcccag tcgaccgtga agccggcctc cttcaggccg acttcgacgc cgttgccgag
      361 gatcggatcg tcctccacca gcaataaacg catcgttccc tcgtcgaacc tgcgcgtctt
      421 atcggcgttc cagcttaagc ggatctgaac gcgcgcggca gaattttttc gcggcttaag
      481 actttcttca gcgctctgcc gcacgctctc accgtgtcga acggcaaccc ggcatccgcc
      541 ggcccgatca ccaaccaatg aagggatcca tcatgaaagc atacgcatcg aaggccgcag
      601 ccgcactggc gctctccgtt ctgtccgtgg gcgcgctcgc ctccacttac aacggcgaat
      661 gcaccgatca gccgaaatcg aagtggatga ctacggccga cgtcaaggcg cgtttcgaag
      721 cccagggcta caacgtcggc cgcgtcaaga ccggcgggag ttgctacgag gtctacgcga
      781 aggacaagaa cggcacgaag gtcgagctct tcgtcaaccc ggtcgacgct cgcgtcgtcg
      841 gccaggcggg gacgcaatga agccggccgc cttcagcgtg gctgcgaccc gggcgccggc
      901 cagggtcgaa acggtccggg tgtgggatcc gctggtccgg gtcttccact ggtcgctggt
      961 ggccgcgttc ttcggtgcct acgcgctcgg agacgacgga ggcgcgctgc accgggcgct
     1021 cgggtacacg gcgctcggac tcgtcggttt ccgcctggca tggggcatcg tcggcggccg
     1081 gtacgcg
//
LOCUS       DASUCJ010000697         1420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_649540, whole genome shotgun sequence.
ACCESSION   DASUCJ010000697 DASUCJ010000000
VERSION     DASUCJ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649540"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1420
                     /locus_tag="VFU90_08325"
     CDS             <1..>1420
                     /locus_tag="VFU90_08325"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479821.1"
                     /translation="DLRRFLSRVVDELSKTIATFGDEAAAILRSDRAMKPDVVVGSLV
                     TDLDQLAGRPTVLALDDYHVIQASEIHDAVRILLDNLPPQATLAIATRADPPLPLPRM
                     RSRGELVEIRAAALRFTPEEAGAFLNDVMELRLQPSQIAALEGRTEGWAAGLQLAALS
                     LRGLDDADRFIAAFTGSHRFVLDYLVEEVLNARSEDVRNFLLETSILHRLTGRLCDAL
                     TGRNDGHQTLEELDRGNVFIVALDDERHWYRYHHLFADSLRARLLAEHPERVPALHVA
                     ASRWYEQTGDLADAIVHALAGADTKRAADLVELALPALRKRREDRTLRNWLQSLPDDV
                     LGSRPLLAAFQAWTRLSEGDLDSVEAWLDDAERTLRAQDNSQASPNAPTALADAVRQR
                     NEEMRALPATIAVYRAAVAQARGDIDATIASARNALNFAEPRDHLSLGAASGFLGLAA
                     WADGDLHTAVDTFSEAVSHLHAA"
BASE COUNT          252 a          483 c          442 g          243 t
ORIGIN      
        1 gacctccgcc ggtttctgag ccgggtcgtc gacgaactgt cgaaaacgat cgcgacgttc
       61 ggcgacgagg ccgcggcaat actgcggtcc gatcgggcga tgaaaccgga cgtcgttgtc
      121 ggcagcctcg tgaccgacct cgaccaactc gccggccgtc caaccgttct tgcgctcgat
      181 gactaccacg tgatccaggc gagtgagatc cacgacgccg tccggatact gctggacaat
      241 ctgcctccgc aggcgacgct cgccatcgcc acacgcgcgg acccgccgtt gccattgcca
      301 cggatgcgca gtcgcggcga actggtcgag atccgagccg cagctctgcg cttcacgccg
      361 gaggaggctg gcgcgttcct caacgacgtc atggaactgc gattgcagcc atcgcaaatc
      421 gccgcacttg agggccgcac cgagggctgg gcggccgggc tgcaactcgc ggcgctttca
      481 ctgcgcgggc ttgatgacgc cgaccgcttc atcgcggcgt tcacgggcag ccatcggttt
      541 gtcctcgact acctggtcga ggaggtgctc aacgcccgat ccgaggatgt gcggaacttc
      601 ctgcttgaaa cgtcgattct gcatcggctg accgggcggc tctgcgacgc gttgaccgga
      661 cgaaacgacg gacatcagac gctcgaagag ctggatcgcg gcaacgtgtt catcgtcgcg
      721 ctcgacgacg aacgccattg gtaccgctac caccatctgt tcgccgattc ccttcgcgct
      781 cggctgttgg ccgaacaccc agaacgggtg ccggcgctgc acgtggccgc gagccgttgg
      841 tacgaacaaa ccggcgatct cgccgacgca atcgttcacg cactcgccgg agctgacacc
      901 aaacgagcgg ccgacctcgt tgaacttgcc ttacctgcgc tgcgcaagcg tagggaggat
      961 cgcacattgc gcaactggct tcagtcgctt ccagacgatg tgcttggcag tcgaccgctg
     1021 ctcgccgcct ttcaagcgtg gacacggttg tccgagggcg atctagacag tgtggaggca
     1081 tggctggacg acgccgagcg cacattgcga gcccaagaca acagccaagc ctcgccgaat
     1141 gcgccgaccg cgttggcgga cgcagtacgt caacgtaacg aggagatgcg cgcgctcccc
     1201 gccaccatcg cggtgtatcg cgcggcggtt gcgcaagccc gcggcgacat cgatgccacc
     1261 atcgccagcg ctcgtaacgc cctcaacttc gctgaaccgc gcgaccacct ctcgctcggg
     1321 gccgcctcag gttttcttgg ccttgccgcg tgggcggacg gcgatctgca cacagccgtc
     1381 gacacgttca gcgaggcggt gtctcacctg catgccgcag
//
LOCUS       DASUCJ010000698         1141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_389547, whole genome shotgun sequence.
ACCESSION   DASUCJ010000698 DASUCJ010000000
VERSION     DASUCJ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389547"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..>1141
                     /locus_tag="VFU90_08330"
     CDS             64..>1141
                     /locus_tag="VFU90_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5479822.1"
                     /translation="MGEVVVVSDPEMVKQLFALSGDEAHAGKANVVVKPFLGKHSLLL
                     LDGPEHARQRKMMVPAFHGERMHAYGAAMLDLAHTAIDTWRQGEPFAVHREMQSITLR
                     IILRTVFGIEGGARFAEFERLLTASLDVASAPILLFPAMQRDLGRFSPWGKFRRLSQK
                     ISRAIREEITRARSGAARGRSDILAMFVDSRDDSGRALDDDEVHDQLVTLLVAGHETT
                     ATALAWTLRWVIEDPSLIARLRAEIADAGADPVRLANLPLLDATIKESLRLQPVLPIV
                     GRILQRPARIGPYEFTPGTAVTAAVYLVHRRAALYPDPHRFRPERFLSFKPASWEWLP
                     FGGGLRRCIGAAFALYEMKMVLAAV"
BASE COUNT          190 a          404 c          355 g          192 t
ORIGIN      
        1 gcgctcctcg ccgacggcca atcgcgcttc ggcgacgcat tccgaatcaa cctgccgggc
       61 cgcatcggcg aggtcgtcgt cgtctcggat cccgagatgg tcaaacagct gttcgcgctg
      121 agcggggatg aggcccacgc cggaaaagcg aacgtcgtgg tcaaaccgtt tctcggcaag
      181 cactccctgc tgctcctcga cgggccggag cacgcgcgtc agcgcaagat gatggtcccg
      241 gcgttccacg gcgagcgcat gcacgcgtat ggcgccgcca tgctcgacct tgcacatacg
      301 gcgatcgaca cgtggcgcca aggcgagccc ttcgcggtgc atcgcgagat gcaatcgatc
      361 acgctacgca tcatccttcg aacggtcttc ggcatcgaag gcggcgcccg gttcgccgaa
      421 ttcgaacgac tgctgaccgc atcgctcgac gtcgccagcg ctccgattct gctcttcccc
      481 gcaatgcagc gagacttggg gcgcttcagt ccgtggggca aatttcgtcg cctgtcgcag
      541 aagatcagcc gcgccatccg cgaagaaatc acccgggcgc gaagcggggc cgcccggggg
      601 cgcagcgaca ttctcgccat gttcgtcgat agtcgcgacg acagcggccg agccctcgac
      661 gacgacgaag tccacgacca gctcgtcacg ctcctcgttg ccggccacga gaccacggcg
      721 acggcgctcg cctggaccct ccgctgggtg atcgaagacc cgtcgctgat cgcccggctg
      781 cgtgcagaaa tcgcggacgc aggggctgat ccggtgcgcc tggcgaacct tccgctactc
      841 gacgccacga tcaaggaatc gctccgcttg cagcccgtac tcccgatcgt cggccgcatc
      901 ctgcagcgac cggcgcgcat cggcccttac gagttcaccc caggtactgc cgtcacggca
      961 gcggtttacc tagtccatcg gcgcgccgcg ctttatcccg acccgcaccg attccgaccc
     1021 gagcggtttc tctccttcaa accggcgagc tgggagtggc tgccgttcgg cggcggcctc
     1081 cggcgatgca tcggagcggc gttcgccctt tacgagatga agatggtcct ggcggcggtg
     1141 c
//
LOCUS       DASUCJ010000699         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_954908, whole genome shotgun sequence.
ACCESSION   DASUCJ010000699 DASUCJ010000000
VERSION     DASUCJ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954908"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFU90_08335"
     CDS             complement(<1..185)
                     /locus_tag="VFU90_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479823.1"
                     /translation="MEDTDARFGRGYLEQQYAQSDNLLVRYAPHARYSEQTSSFRSWV
                     MPHLSIEAGDLVCDAGCG"
     gene            complement(188..>1750)
                     /locus_tag="VFU90_08340"
     CDS             complement(188..>1750)
                     /locus_tag="VFU90_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5479824.1"
                     /translation="DPDTAEPADGAIAEAAPIGAAPPASPTPDVTATLVRWMQYDVMR
                     PFAFFALAALYVLSRAPLLDNGYGTKPDAWRIALSGYWLWEHGQFYPSRLPGYPIPEL
                     SYAAVFKGGWVATNSLTVLVSLVGVWFFAGIVRRLRLPNPALLTVGFAFTPLLWINSM
                     NTMDYTWALTFILGCYYFLLLDNDLAAGIMLGLAVGSRIPSAGMGVPFVLYMWRGGKR
                     DEIRTFLVAVVAVALAAFSPAIWKYGPSFFNFYDAKVGYREVIRLLAKDSLGLAGAAA
                     LLVATIWSLPRLMRLPADFVRDRNVMVWVLAIIITAATFSRLPHEAAYLIPLFPFAWL
                     VVGKYYRTGAIAGAAAVIIFAGFVDFTTTNHEVSASSIRHLRVGEGMLLSNRDTQNSQ
                     RGFTQDVENLKVPDNSLVIVGFVYPQFAMSNRDRLELAILHKDKSAISQLSDKGKAVD
                     AAHNITYVWLLNWDDFNHYRQTVQHIYYTIDADRSTEALYGYRVGEFPDTTVMDFGRT
                     PSGEPDTNSTQR"
BASE COUNT          306 a          578 c          588 g          278 t
ORIGIN      
        1 ccgcagccgg cgtcgcaaac taaatcgccg gcctcgatgc tgagatgcgg catcacccaa
       61 gagcggaacg acgacgtctg ctcgctgtag cgcgcgtggg gcgcgtagcg tacgagcagg
      121 ttgtcggact gcgcgtactg ctgctccagg tagccgcgcc cgaagcgcgc gtccgtgtcc
      181 tccaccgtca gcgctgcgtg ctgttcgtgt ccggctcgcc cgaaggcgtt cggccgaagt
      241 ccatcaccgt cgtgtccggg aactccccta cccggtagcc gtacaacgcc tcggtgctcc
      301 ggtcggcgtc gatggtgtag tagatgtgct ggaccgtctg ccggtagtgg ttgaaatcgt
      361 cccagttgag gagccagacg tacgtgatgt tgtgcgcggc gtccactgcc ttccccttgt
      421 cggacagctg gctgatcgcc gacttgtcct tgtgcaggat cgccagctcg agccggtctc
      481 ggttcgacat cgcgaactgc gggtagacga aaccgacgat cacgagcgag ttgtcgggca
      541 ccttcaggtt ctcgacgtcc tgcgtgaagc cgcgctgaga gttctgcgtg tcacggttcg
      601 agagcagcat gccttcgccg acgcgcaggt gccggatcga tgacgcgctc acctcgtggt
      661 tggtcgtcgt gaagtccacg aagcccgcga agatgattac ggccgccgcg ccggcaatgg
      721 cgcccgtgcg gtagtacttc ccgacgacga gccacgcgaa ggggaacagc gggatcaggt
      781 acgcggcctc gtgtggcagc cggctgaacg tcgcggccgt gatgatgatc gccagcaccc
      841 acaccatgac gtttcggtcg cgcacgaagt ccgccggcag acgcatgagg cgcggcagcg
      901 accagatcgt cgcgaccagc agcgccgcgg cgcccgcgag gccgagcgag tccttcgcga
      961 gcaaccggat cacctcgcgg tacccgacct ttgcgtcgta gaagttgaag aagctcgggc
     1021 cgtacttcca gatcgccggc gagaacgccg ccagcgccac cgcgacgacg gcgacgagga
     1081 acgtgcggat ctcgtcacgc ttgccgccac gccacatgta cagcacgaac ggcacgccca
     1141 tgcccgccga cgggatgcgc gagccgacgg cgagcccgag catgatcccg gccgcgaggt
     1201 cgttgtcgag cagcaggaag tagtaacagc cgaggatgaa cgtcagcgcc cacgtgtagt
     1261 ccatcgtgtt catgctgttg atccacagca gcggcgtgaa cgcgaagccg accgtgagca
     1321 gcgccgggtt cggcagccgc agcctccgca cgatgcccgc gaagaaccat acgccgacca
     1381 gcgaaacgag caccgtgagc gaattcgtcg ctacccaacc gcccttgaat acggccgcgt
     1441 aactcagctc ggggatggga tatcccggca ggcgcgaagg gtagaactgc ccgtgctccc
     1501 atagccagta gccgctgagc gcgatgcgcc atgcgtccgg cttcgtgccg tagccgttgt
     1561 ccagcagcgg cgcgcggctc agcacgtaca gcgcggcgag cgcgaagaac gcgaacggcc
     1621 gcatgacgtc gtactgcatc caccgcacga gcgtcgcggt gacgtcgggg gtcggcgatg
     1681 cgggcggcgc cgcgccgatg ggcgccgctt cggcgatcgc gccgtcagcc ggttctgctg
     1741 tgtccgggtc
//
LOCUS       DASUCJ010000700         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_649553, whole genome shotgun sequence.
ACCESSION   DASUCJ010000700 DASUCJ010000000
VERSION     DASUCJ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649553"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFU90_08345"
     CDS             complement(<1..862)
                     /locus_tag="VFU90_08345"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU5479825.1"
                     /translation="MSGAFVIAPDKFKGSLTATQAAEAIARGIKKVRPDVECRLCPMA
                     DGGEGTVDVFLARGAEAKRVAVRGPLGDPVEATIALRGDTAILEMSSASGMRLLEPAR
                     YDPVRADTFGTGQLIEAALDAGAKRLIVGLGGSATNDAGTGMLRALGVRFRAADGSEI
                     DGPTTAYERLTSIDLRGLDARLAGVRVLAATDVDNPLCGRDGAARTFAAQKGANADEI
                     ERLDAVLSHVADVAAATLRRDERDAPGAGAAGGLGFALIAFLGATAESGVALVAREWG
                     LKTLLHGAVVC"
     gene            complement(876..2399)
                     /locus_tag="VFU90_08350"
     CDS             complement(876..2399)
                     /locus_tag="VFU90_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5479826.1"
                     /translation="MKPLSRILAEGSDERGENALKRSLGPWALTAMGIGAIIGTGIFV
                     LTGVASATRAGPSITISFVVAGIVSALAALCYAEVSSKIPISGSAYTYTYATLGEFLA
                     WIIGWDLVLEYALGAATVSIGWSGYFVNIMHQTAAATGLGWLNIPPEWANGPHWGLAG
                     QAGVSGYANLPAAGIILIITALLARGTRESGTVNGVIVAVKLLIVLFFIAVGLGHIDG
                     ANYHLPAGGPAGLGGYFPFGVAGMLGGAAFIFFAYIGFDAVSTTAEEARNPGRDLPFG
                     IILSLVICTILYIIVVAILNGMVPFYKLNVAFPVAYAMNYVGLSWAGVIISLGAIAGL
                     TTVLLVMMFGQSRIFYAMSRDGLIPPSFTRVHKTWRTPIFSTAFFGICIAAISAFTPI
                     NIVGSLTNMGTLAAFILVSIAVPILRVRHPEVRGQFQLPLGPYVIPVLSAVTALGLIY
                     YLKVGNPVAWGFFPIVWLWFLIWLVVGLAFYFVYGRRKSTVALGETEGLAVAQPPVN"
     gene            2528..>3376
                     /locus_tag="VFU90_08355"
     CDS             2528..>3376
                     /locus_tag="VFU90_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479827.1"
                     /translation="MHEYDVLVVGGGNAGCAAAIAAARHGARTLLVERYGFLGGTATA
                     SMVGPWMTFHSGEDRIVGGIAQEIVERLMARGASPGHLRDSSDYVATVTPFDPEVHKA
                     LLFEMMREAGVALMLHAYVLSARVENGTLRGAAFATVGGIREYAGRVVIDATADAFVA
                     ASAGVETQQGDARGRVQPASLMFRLSHVDLSKLAAYLRTHPDQMRTSLKAHERTAPAL
                     TAVAGLYELWKKAASDGEIDIPRELVSFFISPYPDEVTVNMTRVTDIDPLDPGDLTRA
                     EVEARLQ"
BASE COUNT          615 a         1133 c         1120 g          508 t
ORIGIN      
        1 tgcagacgac ggcgccgtgg agcagcgttt tcagccccca ctcgcgcgcc accagcgcga
       61 cgcccgattc ggcggtcgcg ccgagaaacg cgatcagcgc gaagccgaga cctcccgcgg
      121 ctcccgcccc cggcgcgtcg cgctcgtcgc gccgcagggt tgcggcggca acgtcggcga
      181 cgtgcgacag caccgcatcc agccgctcga tctcatcggc gttcgcgcct ttttgggcgg
      241 cgaacgtgcg cgcggcgccg tcgcgcccgc acaaggggtt gtcgacgtcg gtggcagcca
      301 gaacgcgcac gccggccagg cgcgcgtcga gcccgcgcag gtcgatgctc gtcaaacgct
      361 cgtaggcggt cgtcggcccg tcgatctccg agccgtcggc ggcacgaaag cgaacgccga
      421 gcgcccgcag catgccggtg ccggcgtcgt tggtcgcgct gccccccagc cccacaatca
      481 atcgcttagc gcccgcatcg agtgcggctt cgatcagctg accggttccg aacgtgtcgg
      541 cacggaccgg atcgtaacgg gcgggctcga gcagccgcat acccgaggcg ctcgacatct
      601 cgaggattgc cgtgtcgccg cgcagcgcga tcgtcgcttc gacggggtca ccgagcggac
      661 cgcgaacggc gacgcgtttg gcttccgcac cgcgcgctaa aaaaacgtcg accgtgcctt
      721 cgccgccgtc ggccatcgga cagagccggc actcgacgtc cggccgcacc ttcttgatgc
      781 cgcgcgcgat cgcttccgca gcttgggtcg ccgtcaaact ccctttgaac ttatcgggag
      841 cgatgacgaa ggcgccggac acgggcgcgc gagcgtcagt tcaccggcgg ctgtgcgacc
      901 gccagccctt cggtttcccc caatgccacc gtgctcttac ggcgcccgta gacgaaatag
      961 aacgccaggc cgacgaccag ccagatcaag aaccacagcc acacgatcgg gaagaagccc
     1021 caggcgaccg ggttccccac cttgaggtag tagatcaggc cgagcgcggt aaccgccgac
     1081 agcaccggaa tgacgtacgg gcctaacggc agctggaact ggccgcgcac ttcgggatgg
     1141 cggacgcgca ggatcggaac tgcaatcgag acgagtatga acgccgccag cgttcccatg
     1201 ttggtcagcg agccgacgat attgatcggc gtaaacgcgg aaatcgccgc gatgcagatg
     1261 ccgaagaacg cggtcgagaa gatcggcgtg cgccacgtct tgtggacgcg cgtgaacgac
     1321 ggcggaatca gcccgtcgcg cgacatcgcg tagaagatgc ggctttgccc gaacatcatg
     1381 accagcagca ccgtcgtcag gcccgcaatc gcgccgagcg agatgatcac gccggcccag
     1441 ctcagcccca cgtagttcat ggcgtaggcg accggaaatg cgacgttcag cttgtagaac
     1501 ggtaccattc cgttgaggat ggccacgacg atgatgtaga ggatcgtaca gatgaccaag
     1561 ctcaggatga ttccaaaggg aagatcgcgg cccgggttgc gcgcttcctc ggccgtcgtc
     1621 gaaaccgcgt cgaagccgat gtacgcaaag aagatgaaag ccgcgccccc gagcatgccg
     1681 gcgacgccga acgggaaata gccgcccagg ccggccggac cacccgccgg cagatggtag
     1741 ttcgcgccgt cgatgtgacc cagtccgacc gcgatgaaga acagcacgat caacagcttg
     1801 accgccacga tgacgccgtt gaccgtaccg gattcgcgcg ttccgcgcgc cagcagcgcg
     1861 gtgatgatca ggatgatgcc ggctgcggga aggttggcgt aaccggaaac gccggcttgt
     1921 ccggcgagcc cccaatgcgg cccgttggcc cattccggcg ggatgttcag ccagccgagg
     1981 ccggttgccg ccgccgtttg gtgcatgatg ttgacgaagt agcccgacca gccgatgctg
     2041 acggtggccg ccccgagcgc gtattcgagc acgagatccc acccgatgat ccaggcgaga
     2101 aactcgccga gggtggcata ggtgtaggta taggcgcttc cggagatcgg gatcttgctc
     2161 gagacttcgg cgtagcacaa cgcggccagc gcgctgacga taccggctac cacgaacgag
     2221 atcgtaatcg aagggccggc gcgcgtcgcc gaggcgacgc cggtcagcac gaagatgccc
     2281 gttccgatga tcgcgccgat gcccatggcg gtgagcgccc agggacccag cgagcgcttg
     2341 agcgcgtttt cgccgcgctc gtcgctgccc tcggcaagaa ttcgtgagag gggcttcact
     2401 cggtcaaaca gcagcattgc gcaacggaat acaccctcgg cgtccgactc cctggttttt
     2461 cgccgccgaa cggctgcgag cggcgcgcac gaggcggcgt gcggcgcgcg agcgaacccg
     2521 gcgggggatg cacgagtacg acgtgctggt cgtgggcggc ggcaacgccg gatgcgccgc
     2581 ggcgatcgcg gcggcgcggc acggtgcgcg cacgctgttg gtcgagcgct acggttttct
     2641 cggcggaacc gccaccgcgt cgatggtcgg cccgtggatg acgttccact cgggcgaaga
     2701 tcgcatcgtc ggcgggatcg ctcaagagat cgtcgagcgg ctgatggcgc gcggcgcttc
     2761 gccgggacat ctgcgcgact cctcggacta cgtcgccacg gtcacgccgt tcgaccccga
     2821 agtgcacaaa gcgctgctct tcgaaatgat gcgcgaggcc ggcgtcgcgc tgatgctgca
     2881 cgcgtacgtg ttgtcggcgc gcgtggaaaa cggcaccctg cgcggcgccg cctttgcgac
     2941 ggtcgggggc attcgcgagt atgccggccg cgtcgtgatc gatgccaccg ccgatgcgtt
     3001 cgtcgccgcc tccgcaggcg tcgaaaccca acaaggcgat gcgcgcggcc gcgttcagcc
     3061 cgcaagcctg atgtttcgct tgagtcacgt cgacttgtcg aaacttgccg cctacctccg
     3121 cacgcatccc gatcaaatgc ggacgtcgct caaagcgcac gagcgtaccg cgcccgcgct
     3181 gacggccgtc gccggactct acgaattgtg gaaaaaagcg gcgagcgacg gcgagatcga
     3241 tatcccgcgc gagctggtat ccttctttat ctccccgtat ccggacgaag tgaccgtgaa
     3301 catgacgcgc gtcacggaca tcgatccgct cgaccccggt gatctcaccc gcgcggaagt
     3361 cgaagcgcgc ctgcaa
//
LOCUS       DASUCJ010000701         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1505316, whole genome shotgun sequence.
ACCESSION   DASUCJ010000701 DASUCJ010000000
VERSION     DASUCJ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505316"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFU90_08360"
     CDS             <1..736
                     /locus_tag="VFU90_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEU5479828.1"
                     /translation="FRTEVDYPRIVAYVAWFDHTRTALAWYPGRYEPPRAPVRGPFSV
                     PHDQRWRLLATFNGGFTYVDGQKGSAVNGHTNEPLKDGLATLVAYRNGRVAILRWTGG
                     PNAPANVAWARQSLPPIVWAGKLNPALDNSTKWGMTLGNAVRVWRTGVGIDRRGNLIY
                     AAANDQTVVTLAHILQRAGAVRAMQFDINPEWPTLITYTHDHGLVPNEVVPNPMQPPT
                     RYLVPDDRDFVAVYRRLTGPISVPFG"
     gene            complement(739..>1011)
                     /locus_tag="VFU90_08365"
     CDS             complement(739..>1011)
                     /locus_tag="VFU90_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479829.1"
                     /translation="QVNSVALTFAPGNLLLNAIASAVIGGVSLFGGKGEVRGALLGAV
                     LIGTLQNGLNTLNVSNGWIYIITGLVLLGAVTLDTFAVRLQARSGR"
BASE COUNT          171 a          353 c          339 g          148 t
ORIGIN      
        1 gttccgcacc gaggtcgact atccccgcat cgtcgcgtac gttgcgtggt tcgatcacac
       61 gagaaccgcg cttgcgtggt atccgggccg gtacgagccg ccgagggcgc ccgtccgcgg
      121 tccgttctcc gtcccgcacg atcagcgctg gcggctgctc gcaaccttca acgggggctt
      181 cacgtacgtc gacggccaga aggggtcggc cgtgaacggg cacacgaacg aaccgctcaa
      241 ggacgggctt gccaccctcg tcgcctatcg caacggacgc gtcgcgattc tccggtggac
      301 cggcggtccg aatgcgccgg cgaacgtcgc ctgggcacgg cagagccttc ccccgatcgt
      361 ctgggcgggg aagctgaacc ccgcgctcga caacagcacg aagtggggca tgacactcgg
      421 gaacgcggtg cgcgtgtggc gcaccggagt cggcatcgac cgtcgcggga acctcatcta
      481 cgccgctgcg aacgaccaga cggtcgtcac gctcgcgcac atcctgcaac gcgccggcgc
      541 cgtgcgcgcg atgcaattcg acatcaaccc ggagtggccg acgctcatca cgtacaccca
      601 cgaccacggc ctcgtcccga acgaggtggt gccgaatccg atgcagccgc cgacgcgcta
      661 cctcgtcccg gacgatcggg acttcgtcgc cgtctaccgc cggctgacgg gtccgatcag
      721 cgtcccgttc gggtaggcct aacgcccgga gcgcgcctgc aggcgcaccg cgaacgtgtc
      781 gagcgtaacg gcgccgagga gcacgaggcc cgtgatgatg tagatccagc cgttcgacac
      841 gttcaacgta ttgaggccgt tctggagcgt cccgatgagg acggcgccga ggagcgcgcc
      901 gcgcacctcg cccttgccgc cgaagaggct gacgccgccg atgactgcgg atgcgatggc
      961 gttcagcagg aggttcccgg gcgcgaacgt gagcgcgacg gagttcacct g
//
LOCUS       DASUCJ010000702         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_939851, whole genome shotgun sequence.
ACCESSION   DASUCJ010000702 DASUCJ010000000
VERSION     DASUCJ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939851"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFU90_08370"
     CDS             <1..267
                     /locus_tag="VFU90_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479830.1"
                     /translation="VIGGTDIAMHQRRLITSLQAIADCIQKIEPDANNRTGVMLAAPV
                     GAIAQLYGVDAVREELAGIVANDEFWNYQRDVAADIAKIRGPKT"
     gene            264..1139
                     /locus_tag="VFU90_08375"
     CDS             264..1139
                     /locus_tag="VFU90_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479831.1"
                     /translation="MTKKKEELPPYHELFELPEGETPQTFAPPPNFSAIKLWVKRFGP
                     NGLEECPRPWLLSEIPDRASFFDMFGGGRYEVEGRTLGGQFYARRTFVLVGDPKPLVP
                     RSASEDKPAAAPAAGGFSMPTGGGGDMASMFAAASGNPMALLFLVMMQNADRAEQRAD
                     RQANQSVEMFKAIATIMGGQSRGTDPAIANVFGSMTELVKAGIQQRPAEQPAQKHLTV
                     EEEIARAKQLIELARKTNPDEKPERIVDILKEIVPMIPPQVFQGLLQPVAGAANGSGI
                     IVEQAGSVVGGGSVG"
BASE COUNT          253 a          398 c          398 g          201 t
ORIGIN      
        1 gtgatcggcg gcaccgacat cgccatgcat caacggcgtt tgatcacgtc gcttcaagcg
       61 atcgccgatt gcattcagaa aatcgagccc gacgcgaaca atcgaacggg cgtcatgctc
      121 gccgcgcccg tcggcgcgat cgcgcagctc tacggcgtcg acgccgtgcg cgaagagctc
      181 gccggcatcg tcgcgaatga cgaattttgg aactatcagc gcgacgtcgc cgccgacatc
      241 gcgaaaatcc gagggccgaa gacatgacga agaaaaaaga agaattgccg ccgtatcacg
      301 agctcttcga gctgcccgaa ggcgaaacgc cgcaaacgtt cgcgccgccg ccgaatttca
      361 gcgcgatcaa gctgtgggtg aaacgcttcg gaccgaacgg gctcgaagag tgcccgcgcc
      421 catggcttct ttccgagatt cccgatcgcg cgagcttctt cgacatgttc ggcggcggcc
      481 gctacgaagt cgaaggccga acgctcggcg ggcaattcta cgcgcgacgc acgttcgtgc
      541 tcgtcggcga tccgaaaccg ctcgtgccgc gctcggcgtc cgaagacaag cccgccgcgg
      601 cgccggccgc gggcggtttt tcgatgccga cgggcggcgg cggcgacatg gcgagcatgt
      661 tcgcggccgc gtcgggaaat ccgatggcgc ttcttttcct cgtgatgatg cagaacgccg
      721 atcgcgccga gcagcgcgcc gatcgtcaag caaatcagtc ggtcgaaatg ttcaaagcga
      781 tcgctacgat catgggcgga caaagccgcg gcaccgatcc ggcaatcgcg aacgtcttcg
      841 gcagcatgac cgagctcgtg aaagccggaa tccagcaacg gcccgccgag cagcccgcgc
      901 aaaagcacct gaccgtcgaa gaagaaatcg cgcgcgcgaa acaattgatc gagctcgcgc
      961 gcaaaacgaa tcccgacgaa aagcccgaaa ggatcgtcga catcttgaag gaaatcgtgc
     1021 cgatgattcc gccgcaagtc ttccaaggct tgctgcagcc ggtcgcgggc gcagcgaacg
     1081 gatccggcat catcgtcgag caagcgggca gcgtcgtcgg cggcggatcc gtcggatgat
     1141 ttccggcgcg cagctcttct tcgccgtgtg gatcggctgc gcgtgcgtct acgccgtgcc
     1201 cgtgtgggcg accttgtcgc tttccaaggc gcggcgcgcg tgcggtgcgt
//
LOCUS       DASUCJ010000703         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_129912, whole genome shotgun sequence.
ACCESSION   DASUCJ010000703 DASUCJ010000000
VERSION     DASUCJ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129912"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..>1576)
                     /locus_tag="VFU90_08380"
     CDS             complement(224..>1576)
                     /locus_tag="VFU90_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5479832.1"
                     /translation="VDGTKLELGRDFLPASIAATHRDIANVPVVYGGSLGDPSSLIGP
                     EQSADRLVVLMPRDASLRGLRLDPRRFAGAKAIAIAMLDDLPPEFIARLRDGRPVPDT
                     TTNASIVPILWISRSAAAKLLGSASEGLSPGAEGQRVSGSFGFEHRPVPFPARNVVAI
                     LRGADPALRGEYVSLTAHNDHVGFDHSPVDHDSLRAFNRVIRPMGADSPLREPTAEEW
                     TRIRAILDSLRRVHPARLDSIRNGADDDGSGTVSILEIAESMARGPRPRRSILFVSHT
                     GEEDGLVGSRWYTDHATVPLDSVIGEIDQDMVGRALPTDFPHDGTGAGSPTYLEVIGF
                     KRLSREFGEALDSANAHQPVPFVFNLEYDQPGNPLQYYCRADHFSYARYGIPAVALSR
                     GEHLDYHQVTDEAQYIDYPDLARVSKMVLDMATRLANAPERPKLDVPKPADPHGPCKQ
                     "
BASE COUNT          266 a          523 c          520 g          267 t
ORIGIN      
        1 gttgttggag acctgcacga tacgcttcgc gatagcccag ccgttcatcg gctccagcga
       61 aatcgttttc agaatgagca ggtccagcgt gccttgcagc agcggcgtgt gacgggccat
      121 gggtctcctg tcggcactcg atacgaatat cggtcgcgct cgtatcgaat gtcaatagga
      181 gcgatagtac cgagagcctc gcgcgcctcc gtccgatcag ccactactgc ttgcagggcc
      241 cgtgcggatc cgccggtttc ggcacgtcca gtttcggccg ttccggcgcg ttcgccagcc
      301 gggtggccat gtccagcacc atcttcgaca cgcgtgccag atcgggatag tcgatgtact
      361 gcgcttcgtc cgtcacctgg tggtagtcga ggtgctcgcc ccgcgagagc gcgaccgccg
      421 gaataccgta tcgcgcgtag ctgaaatgat cggcgcggca atagtactgg agtgggttcc
      481 ccggttgatc gtactccaga ttgaacacga acggcaccgg ctgatgagca tttgctgaat
      541 cgagcgcctc gccgaactcg cgggacaggc gcttgaaccc gatcacctcg agatacgtcg
      601 ggctccccgc gcccgtgccg tcgtgcggga agtccgtcgg cagcgcgcgg ccgaccatgt
      661 cctggtcgat ctcgccgatc accgaatcca gcggtaccgt cgcatggtcc gtgtaccacc
      721 gagatcccac cagaccgtcc tcctcgccgg tgtgactcac gaagaggatc gaccgccgag
      781 gccgcggacc gcgcgccatc gactcggcaa tttcgagaat gctcaccgtg cccgagccgt
      841 cgtcgtcggc gccgttgcga atcgagtcga gccgcgccgg gtgcacccgc cgcaggctgt
      901 cgagaatggc gcgaatgcgc gtccactcct ccgccgtcgg ctcgcgaagc ggcgagtcgg
      961 cgcccatggg gcggatcacc cggttgaacg cccgcagcga atcgtgatcg accggcgaat
     1021 ggtcgaagcc gacgtgatcg ttatgcgccg tgagcgacac gtactcgcct cggagcgccg
     1081 ggtcggcacc gcgcaggatc gcgacgacat tgcgcgccgg gaatggtacc ggccggtgct
     1141 cgaatccgaa gctgccgctc actcgctgcc cttcggcccc cggcgaaagt ccctcggatg
     1201 cgctgccgag aagcttggcg gcagccgaac gagagatcca gaggatcggc acgatcgacg
     1261 cgtttgtcgt cgtgtccgga accggccgtc cgtcgcgcag ccgcgcgatg aattccggcg
     1321 gcaggtcgtc gagcatggcg atggcaatcg ccttcgcgcc ggcgaagcgc cgcggatcga
     1381 ggcgcaggcc gcgcagcgat gcgtcgcgcg gcatgagcac gacgagccgg tccgccgact
     1441 gctccggccc gatgagcgag cttgggtcgc ctaacgagcc gccgtagacg acaggtacat
     1501 tcgcgatgtc gcgatgtgtc gccgcgatcg atgccggaag aaagtcgcgg ccgagctcga
     1561 gcttcgttcc gtcgac
//
LOCUS       DASUCJ010000704         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_695118, whole genome shotgun sequence.
ACCESSION   DASUCJ010000704 DASUCJ010000000
VERSION     DASUCJ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695118"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1076
                     /locus_tag="VFU90_08385"
     CDS             <1..>1076
                     /locus_tag="VFU90_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479833.1"
                     /translation="IYPELVFPRITIVAQGSALGAQQIVFSITRPLEEAVSVVPGVSR
                     VDSKTIRGSSQIDVTFTPNTDMIYALQLVQARVNEARQQLPPGIQIDVARLTPSLFPI
                     LSYNLEGGDPATLYDIARYQIKPLLSRIPGVGLVEVQGSDVREIEVVADPERLASAGL
                     SYDDLAAAIDSAITPQAVGRVARDYRQYLIVTDNEAHDVDDVANVVVSDGLRVRDLAT
                     VMLGTEDHVSVIAGDGRPAALLNVTRQIGGNTLLIADSVAAIARQVQRTLPPGVRLKP
                     VYDQASLVRDAVRSVRDAMLVGAVLAVLILLVFLRHARITAISATSIPLTLMITVFVM
                     RLLGQTFNLMTLGAMAIAIGLVID"
BASE COUNT          165 a          362 c          362 g          187 t
ORIGIN      
        1 catctacccc gagctcgtgt tcccgcgcat cacgatcgtc gcccaaggct ctgcgttagg
       61 cgcgcagcag atcgtgttca gcatcacgcg gccgctcgag gaagcggtga gcgtggtgcc
      121 gggcgtcagc cgcgtcgatt cgaagacgat tcgcggttcg tcgcagatcg acgtgacctt
      181 cacgcccaac acggacatga tctacgccct gcagctcgtg caggcgcgcg tgaacgaagc
      241 ccggcagcag ctgccgccgg gcattcagat cgacgtggcg cggttgacgc catcgctctt
      301 tccgattctg tcgtacaatc tcgagggcgg cgatccggcc acgttgtacg acatcgcgcg
      361 gtaccagatc aagccgctgc tctcgcgcat cccgggtgtc gggctggtcg aggtgcaggg
      421 gagcgatgtc cgggaaatcg aagtggtggc cgaccccgaa cggctggcgt cggcgggcct
      481 gtcgtacgat gacctcgcgg cggcgatcga ctccgcgatc acgccgcagg ccgtcggtcg
      541 cgtcgcacgc gactaccggc agtatctcat cgtcaccgac aacgaggcgc acgacgtcga
      601 tgacgtggcc aacgtggtcg tgtccgacgg attgcgggtg cgcgacctcg ccacggtcat
      661 gttaggcacg gaggaccacg tgagcgtcat cgccggcgat ggccgtcccg cggcgctgct
      721 caacgtgacc cggcagatcg gcggcaacac gctgctcatc gccgacagcg tcgcggcgat
      781 cgcccggcag gtccagcgca cgctgccgcc gggcgtccgg ctcaagccgg tctacgatca
      841 ggcctcgctg gtgcgcgacg cggtgcggtc ggtgcgcgat gcgatgctcg tcggcgccgt
      901 gctcgcggtg ctgatcctgc tcgtgttcct ccggcacgcg cgcatcaccg cgatcagcgc
      961 gacgtcgatt ccgctcacgc tcatgatcac cgtgttcgtg atgcggctgc tcggccagac
     1021 gttcaatctg atgacgttag gcgcgatggc catcgccatc ggcctggtga tcgacg
//
LOCUS       DASUCJ010000705         1426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_160347, whole genome shotgun sequence.
ACCESSION   DASUCJ010000705 DASUCJ010000000
VERSION     DASUCJ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160347"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFU90_08390"
     CDS             complement(<1..469)
                     /locus_tag="VFU90_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479834.1"
                     /translation="MLPRLTIGFIVLSAAIAIAACSSYGAGSSVNVGPNFPPKTLYAS
                     NSNQNAISIYNKGQKTGTGPAFEIGGSSTTLNGPQYLAFDHGQNLWITNYNPSTNRAL
                     LVEIEALATGNVVPLQSVALPGHVRGVAISTQKKGQTSLMVIADVIPTEKYPSQ"
     gene            complement(551..703)
                     /locus_tag="VFU90_08395"
     CDS             complement(551..703)
                     /locus_tag="VFU90_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479835.1"
                     /translation="MPRQIIDTESSRPAYVRRRVTTFVITAVVVLAIVAAAWFLWGRY
                     AVAGAH"
     gene            complement(710..>1426)
                     /locus_tag="VFU90_08400"
     CDS             complement(710..>1426)
                     /locus_tag="VFU90_08400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5479836.1"
                     /translation="LVTPEMNVHNEIADDLPARAYRVTQESAPEDRSNRPKLIALTFD
                     DGPYPVFTPMLLDVLRDERVPATFFLIGRDAEQWPELTHRIVQDGHEVADHTYTHPDL
                     DREPDDAVRAEILQGRDALWKLTHDPAVRTLMRPPHGRYTERTLEIAQSLGYSVVLWT
                     DDSGDWRTLTPAEIERHLLVHATAPEIVLLHSGKLATIEAMPEVIERFEKAGYRFVTV
                     SELLARVTAHELNHPLRRAV"
BASE COUNT          226 a          454 c          478 g          268 t
ORIGIN      
        1 cttggctggg atatttctcg gtcgggatga cgtcggcgat taccatcagc gacgtttggc
       61 cttttttctg ggtcgagatg gcgacgccgc gcacgtgccc gggcagcgcg accgactgca
      121 gcgggacgac gttgccggta gcgagcgcct cgatttccac cagcagcgcg cgattggtcg
      181 aaggattgta gttggtaatc caaagattct ggccgtggtc gaacgcgaga tactgcgggc
      241 cgttgagggt cgtgctcgat ccgccgattt cgaaagcggg tccggttccg gtcttctgcc
      301 ccttgttgta gatactgatg gcgttctggt tgctgttgct cgcatacagg gtcttcggcg
      361 ggaagttcgg cccgacgttc accgacgaac cggcgccata cgagctgcac gcagcgatcg
      421 cgatcgccgc gctgagcacg atgaacccta tggtaagcct aggaagcatt ctttctcctc
      481 ggggttgggg gatcgcaccg gaaccggtcc caggttcccc tagtgcgaag actttcctgg
      541 aagcggcgcc ttaatgggcg cccgccacgg cgtagcggcc ccaaaggaac caggcggccg
      601 caacgattgc cagcacgacg acggccgtta tgacgaaggt cgtgactcgc cggcgcacgt
      661 aggccggccg gctggattcg gtatcgatga tctggcgggg cacgcgggct taaaccgccc
      721 gccgcaacgg atggttcagc tcgtgggcgg tgacccgggc cagcagctcg ctcaccgtca
      781 cgaaccggta cccggccttt tcgaagcgct cgatcacctc gggcatggcc tcgatggtgg
      841 cgagcttccc gctgtgcagc agcacgatct cgggcgcggt cgcgtgcacg agcaggtgcc
      901 gctcgatctc ggcgggcgtc agcgtccgcc agtcgccgct gtcgtcggtc cacagcacca
      961 ccgagtaacc cagcgattgg gcgatctcca gcgtccgctc ggtgtagcgg ccgtgcggcg
     1021 ggcgcatcag cgtgcgcacc gccgggtcgt gcgtcagctt ccaaagtgca tcgcggccct
     1081 gcagtatctc cgcgcgcacc gcgtcgtcgg gctcgcgatc gagatccgga tgcgtgtacg
     1141 tgtgatcggc cacctcgtgg ccgtcttgca cgatccggtg cgtcaactcc ggccactgct
     1201 cggcatcgcg accgatcaga aagaacgttg ccggaacgcg ttcgtcgcgc agcacgtcga
     1261 gcagcatcgg ggtgaacacc gggtacggac cgtcgtcgaa ggtcagcgcg atcagtttcg
     1321 ggcggttcga acgatcctcc ggcgccgact cctgcgtcac gcggtacgcg cgcgccggca
     1381 agtcgtcggc gatctcgttg tggacgttca tctccggcgt caccag
//
LOCUS       DASUCJ010000706         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_832618, whole genome shotgun sequence.
ACCESSION   DASUCJ010000706 DASUCJ010000000
VERSION     DASUCJ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832618"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /gene="truB"
                     /locus_tag="VFU90_08405"
     CDS             complement(<1..687)
                     /gene="truB"
                     /locus_tag="VFU90_08405"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5479837.1"
                     /translation="MNAPTSSLTNPNGENAPQAGRVKRQKRDIHGWLVLDKPVGMTST
                     HAVSVIKHLFSARRAGHAGTLDPLASGCLPIALGEATKTVPFVFDARKVYCFTIRWGE
                     ERDTDDAEGRVVATSAERPDRDAIERLLPRFTGTIEQVPPRFSAVKLGGERAYDLARD
                     GEAVELAPRTIKVHRLELMEIPDPDHAVLTAECGKGTYIRSLARDLGRELGTLGHVCG
                     LRRTRVGSFGE"
     gene            complement(785..>1113)
                     /gene="rbfA"
                     /locus_tag="VFU90_08410"
     CDS             complement(785..>1113)
                     /gene="rbfA"
                     /locus_tag="VFU90_08410"
                     /inference="COORDINATES: protein motif:HMM:NF001802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5479838.1"
                     /translation="ADMLARGEVHDSVIEHHMITVPEVRVTADLRLATIYVMPLGGRD
                     EKEVLDALERNSRYVRGEIARRVNLKFAPEIRFRIDERFDEAERIEKLLRTPAVQRDL
                     RSRNEK"
BASE COUNT          184 a          330 c          365 g          234 t
ORIGIN      
        1 ttcgccgaag gatcccacgc gggtacgtcg cagaccgcag acatgcccaa gcgtgccaag
       61 ctcccgcccc aggtcgcggg cgagtgagcg tatgtaagtt cctttgccgc attcggcggt
      121 taaaacagcg tggtcgggat cgggaatttc catcaattca agccggtgga ccttgatcgt
      181 gcggggagcg agttcaacgg cttcgccgtc ccgggccaga tcgtaggcgc gctcgccacc
      241 gagtttgacg gccgaaaaac gcggcgggac ttgctcgatc gtgccggtaa agcgcggcag
      301 cagccgttcg attgcgtccc ggtcgggacg ctcggcgctg gttgccacga cccggccctc
      361 ggcgtcgtcg gtgtcgcgtt cttcgcccca gcggatcgtg aagcagtaga cctttcgcgc
      421 gtcgaacacg aacggcaccg tcttggttgc ctcgccgagc gcgatcggta ggcatcccga
      481 ggccagcggg tcgagcgttc cggcatgacc ggcgcggcgc gccgagaaca ggtgtttgat
      541 gactgagacg gcatgggttg aggtcatgcc gacaggtttg tcgagcacga gccagccgtg
      601 gatgtcccgc ttttgccgtt tgacgcggcc ggcctgcgga gcgttctcgc cgttgggatt
      661 ggtgagcgag ctggtcggcg cgttcatgct ttggaaacca taattcaagc gagcggtaat
      721 ttatcggcgc tcggcgtacc cgccgccggc caggaattcg tcgcaacggc gttgatgtgg
      781 tccattattt ctcgtttcgg cttctcaggt cgcgctggac cgcgggcgtt cgcaaaagct
      841 tctcgattcg ttccgcttcg tcgaatcgtt cgtcgatgcg aaatctgatt tcaggtgcaa
      901 atttgaggtt aacgcgccgt gcgatctcgc cgcgaacgta ccggctgttg cgttcgagcg
      961 cgtcgagcac ttccttttca tcgcgtccgc caagcggcat gacgtaaatc gtcgccaggc
     1021 gcaggtcggc agtgacgcga acttccggaa cggtgatcat gtggtgttcg atcaccgaat
     1081 cgtggacctc gccgcgtgca agcatgtcgg cca
//
LOCUS       DASUCJ010000707         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1291261, whole genome shotgun sequence.
ACCESSION   DASUCJ010000707 DASUCJ010000000
VERSION     DASUCJ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291261"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="ccsA"
                     /locus_tag="VFU90_08415"
     CDS             complement(<1..506)
                     /gene="ccsA"
                     /locus_tag="VFU90_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5479839.1"
                     /translation="MTKMSLDEVLMVTALGSYVLAALALLAYFFSRQDWLRLFGMPLA
                     IIGCAAQFAQLIARFELTGVWPLLNLYGSLSLFAAMAVAIFIGFAFRYRLWFAGGFVL
                     ALAAIFLAYGVTWNEGTMPAVPSLQSYWAKVHVPIVVSSYAAFLVAFVFSCVYLLKYY
                     AERAFEGGV"
     gene            complement(503..1924)
                     /locus_tag="VFU90_08420"
     CDS             complement(503..1924)
                     /locus_tag="VFU90_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEU5479840.1"
                     /translation="MARDGVIGELRPALTGAYDDFVRTFGNVLFAVSLFGVWGLLTLV
                     GVIVDQGKDPSAYFGAYAAPIARAILRLGFDNVYHSPWYVGIIGLILLSLTVCTFKRV
                     IPARLPRLHPVKIDKIPLHATFEADGSPETVRQKLGAFLSGRGWQVRGRTFDGTEWTF
                     ADKHNWARRGVLVAHVGFVIVAAGTTLYWARGFSGEAAVLTGQTVVVPQTHATLRLDS
                     FAYKITPIATKSGIVYQPIDYVSHVTVTGNDGVAKHLTVRVNHPIDVDGTLYYQATYG
                     FGVTFAVTHRGRPVPQLSGRTLLEGDSLDLPGTQRSVVYERFVPTFDRQTGMPGADPR
                     VNNPGAILSIAQAGNSLGDALVPIGTWIDAGDGWQVVPKRYVVFSGFQYRYDPGIPLV
                     GIGAFVLLTGLIVSFYFLPARLYVRVDATAPSRSRVGVAATTVKGYDVFENEFGRLVV
                     ALTKDSGGPGGAAAGAPDWGLAT"
     gene            complement(1906..2673)
                     /gene="tatC"
                     /locus_tag="VFU90_08425"
     CDS             complement(1906..2673)
                     /gene="tatC"
                     /locus_tag="VFU90_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5479841.1"
                     /translation="MLLQQPATRPDEEAPWDQKEMPFTEHLRELRSRLLISIATVGGI
                     AVLLFWPSQFLIKWMMDQYFHGVTLHAFGPADVIFTEFKLSIVGGIVVGMPVLLHQLW
                     MFVVPAVHPRTRRMVYAFVLPSMLLALAGLAFAHFVVIPRVVAALLSITNAVATPTFG
                     VASTLNFVIILLALFAIVFQTPIVLVGLARLGIVNAPMLMRYRRHAIFAFFVAGGIAA
                     PDGNPMTMALLAIPMYVLYEISIWVILLLERTWRATA"
     gene            complement(2681..2983)
                     /locus_tag="VFU90_08430"
     CDS             complement(2681..2983)
                     /locus_tag="VFU90_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5479842.1"
                     /translation="MAIHLEMGLIDAPIIIGILVVAALLFGADKLPKLARSAGQAKKE
                     FVMGQIEADEAADRAREEARQRTAEQHADLMNNVEAGSMTGESVPPPTPGKGTLSS"
     gene            complement(3005..>4019)
                     /locus_tag="VFU90_08435"
     CDS             complement(3005..>4019)
                     /locus_tag="VFU90_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5479843.1"
                     /translation="RGRRDRRAPRNKAVKDALAKNVRAGASGDLHGLVETFFRSTFST
                     DNPLITEAIKRMLAAGGKRLRPRITLLSAAACGGEAVEHLHLAAYMELIHVATLIHDD
                     VVDNARTRRGVNATAVDYGNRVSVLAGDYLFAWIFKNVTADYPHPIPNILSATLADIC
                     DGEVLQLRALGNLALTPSAYVEIARKKTASLFAAAAECGAIMGGGNRDEVAALRAFGD
                     AFGIAFQMKDDLLDITADERSLGKPVGNDLTERKVTIPLVEALAAGDGHFRKAAGRFF
                     DGDPGETAASIVAGIERHGGLAGTRARIAGLVERAKAALEPIAWDAAKEELAKLADAL
                     IAG"
BASE COUNT          760 a         1322 c         1292 g          645 t
ORIGIN      
        1 acccctcctt cgaacgcgcg ctcggcgtag tacttcaaga ggtagacgca cgagaaaacg
       61 aaggccacca ggaaggccgc gtaggacgag accacgatcg gaacgtgaac cttcgcccag
      121 tacgactgca gcgacgggac cgcgggcatc gttccttcgt tccaggtgac gccgtacgcg
      181 agaaaaatcg cggccagcgc gagcacgaac cctccggcga accagagacg gtagcgaaac
      241 gcaaatccga tgaagatggc caccgccatt gccgcaaaga gcgagagcga gccgtagaga
      301 ttgagcagcg gccagactcc cgtaagctcg aagcgcgcga tcagctgggc aaactgcgcc
      361 gcgcagccga tgatggccag cggcatcccg aacagccgca gccagtcttg gcgggagaag
      421 aaatacgcca gcagcgcgag cgccgcgagg acgtaacttc cgagcgccgt taccatcagc
      481 acttcgtcga gcgacatctt ggtcatgtgg ctaaccccca atccggcgcg cccgcggcgg
      541 cgccgccggg accgccggaa tccttcgtca acgctaccac cagccggccg aactcgttct
      601 cgaaaacgtc gtagcccttc accgtcgtgg ctgcgacgcc cacgcggctg cggctgggag
      661 ccgtggcatc gacccgcacg tacagccgcg ccggcaggaa gtagaaggaa acgatcagtc
      721 ccgtcagcag gacgaacgcg ccgattccca ccagcgggat gccgggatcg tagcggtact
      781 gaaagccgct gaacacgacg taacgcttcg gaaccacctg ccagccgtcg ccggcgtcga
      841 tccacgtgcc gattggaacg agggcgtctc ccagcgagtt gccggcctgc gcgatcgaga
      901 gaatcgcgcc cgggttgttc acccgcggat cggcccccgg catccccgtc tgccggtcga
      961 acgtcgggac gaagcgttcg tagacgacgc tgcgctgcgt ccccggtagg tccagcgaat
     1021 cgccctcgag gagggtgcgc ccggagagct gcgggaccgg ccggccgcga tgcgttaccg
     1081 caaaggtgac gccgaagccg tacgtggcct gatagtagag cgtgccgtcg acgtcgatcg
     1141 gatggttgac gcgcaccgtc agatgtttcg caacgccgtc gttgccggtg acggttacgt
     1201 gcgacacgta gtcgatcggt tgatacacga tcccgctctt ggtggcgatc ggcgtgattt
     1261 tgtacgcgaa tgaatcgagc cgcagcgttg cgtgggtttg cggaacgacg accgtttggc
     1321 cggtcagcac cgccgcttca cccgagaaac cgcgcgccca ataaagcgtc gttccggccg
     1381 cgacgatcac gaaccccacg tgtgccacga gcacgccgcg gcgcgcccag ttgtgcttgt
     1441 cggcaaacgt ccattcggta ccgtcgaacg tgcgcccgcg gacctgccag ccacgcccgc
     1501 tcaagaacgc gccgagcttc tgccgcaccg tctcgggact gccgtccgcc tcgaaggtcg
     1561 cgtgcaacgg tatcttgtcg attttgacgg ggtgcaggcg cggcagccgc gccggaatga
     1621 cccgcttgaa cgtgcatacc gtcagcgaca gcagaatcaa tccgatgatg ccgacgtacc
     1681 acggcgagtg gtatacgttg tcgaatccca gccgaaggat cgcgcgggcg atcggcgccg
     1741 cataggcgcc gaagtacgcg ctcggatcct tgccttgatc gacgatcacg ccgacgagcg
     1801 tgaggagtcc ccagacgccg aacagcgaaa ccgcaaacag cacgttgccg aacgtgcgga
     1861 cgaaatcgtc gtaggcaccc gtcagcgccg gccgcagttc gccgatcacg ccgtcgcgcg
     1921 ccacgttcgc tcgagcagca gaatcaccca gatcgatatt tcgtagagca cgtacatcgg
     1981 aatcgccagc agcgccatgg tcatgggatt accgtccggt gcggcgatcc cgccggcgac
     2041 gaagaacgcg aagattgcgt ggcggcgata gcgcatcagc atcggcgcgt tgacgatgcc
     2101 cagtcgcgcc aggccgacga ggacgatcgg cgtttgaaag acgatcgcaa acagcgcgag
     2161 taagatgatg acgaagttca gcgtcgaggc gaccccgaag gtcggcgtcg cgaccgcgtt
     2221 ggtgatactg agcaacgccg ccacgacgcg cggaatgacg acgaagtggg cgaacgccag
     2281 gccggcgagc gcgagcagca tggacggcag gacgaacgcg tacaccatgc ggcgcgtgcg
     2341 cggatgaacg gccggaacga cgaacatcca gagctggtgg agcagtaccg gcatgccgac
     2401 cacaatgccg ccgacgatcg acagtttgaa ctcggtgaag atcacgtccg ccggtccgaa
     2461 tgcgtgcagg gttacgccgt ggaagtattg gtccatcatc cacttgatga ggaactgcga
     2521 cggccagaag agcaaaacgg cgatgccgcc gacggtcgca atcgagatca gaagacggct
     2581 acggagctcc cgaaggtgct ccgtgaaagg catctctttc tggtcccacg gcgcctcttc
     2641 gtcggggcgg gtcgcgggct gctgcagcag cacggcgtgc ttacgagctc agcgtacctt
     2701 taccgggcgt cggcggcggc accgattcgc cggtcatcga ccccgcttcg acgttgttca
     2761 tgaggtcggc gtgctgctcg gcggtccgct ggcgcgcctc ttcgcgggcc cgatcggcgg
     2821 cctcgtccgc ctcgatctgc cccatcacga actctttctt tgcctgtccg gcgctgcgcg
     2881 cgagcttcgg cagcttgtcc gcgccgaaca gcagcgcggc cacgacgaga atgccgatga
     2941 tgatcggtgc gtcgatgagt cccatttcca gatgtatcgc catgaccgtc tccttgctgt
     3001 cgccctatcc tgcgatcagg gcgtcggcga gtttcgccaa ttcttccttc gccgcgtccc
     3061 acgcaatcgg ctccaacgcg gccttcgccc gttccaccaa cccggcgatc cgcgcgcggg
     3121 ttcccgccag gccgccgtgc cgctcgatgc cggcgacgat cgacgcggcc gtttcaccgg
     3181 ggtcgccgtc gaagaaacgg ccggccgcct tgcgaaagtg tccgtcgcct gccgcgagcg
     3241 cctcgaccag cggaatagtc accttgcgct cggtcaaatc gtttccgacc ggcttaccga
     3301 gagagcgttc gtccgccgtt atgtccagca ggtcgtcttt catttgaaat gcgattccga
     3361 acgcgtcccc gaaggcgcgc aacgcggcca cttcgtcccg gttcccgccg cccatgatcg
     3421 ccccgcactc ggcggctgcc gcaaagagcg acgccgtctt cttgcgcgcg atctcgacat
     3481 acgcggacgg cgtcaaggcc aggttgccga gcgcgcgcag ctgcagcact tcgccgtcgc
     3541 agatgtcggc gagcgtcgcc gataaaatgt tcggaatcgg atgcggatag tcggcggtga
     3601 cgttcttgaa tatccaagcg aacagatagt cgccggccag cacgctcacg cgattgccgt
     3661 aatcgacggc cgtggcgttg accccgcggc gcgtgcgcgc gttgtcgacg acgtcgtcgt
     3721 gaatcagcgt ggcgacgtga atgagctcca tgtacgcggc cagatggagg tgttcgactg
     3781 cctcgccgcc gcaggcggcg gccgagagca gcgtgatgcg ggggcgcaga cgctttccgc
     3841 cggccgccaa cattcgcttg atcgcctcgg tgatgagcgg gttatcggtc gaaaacgtgg
     3901 accggaagaa cgtctccacc aatccgtgga ggtcgccgga agcgccggcc cggacgtttt
     3961 tcgcgagcgc gtccttcacg gctttattcc gtggtgcacg gcgatcgcgc cgcccacga
//
LOCUS       DASUCJ010000708         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1398314, whole genome shotgun sequence.
ACCESSION   DASUCJ010000708 DASUCJ010000000
VERSION     DASUCJ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398314"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFU90_08440"
     CDS             complement(<1..294)
                     /locus_tag="VFU90_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5479844.1"
                     /translation="MKLYLSCDMEGTAGVSAWQQCDPSDAYEYPIYRRYMTREVRAAI
                     DGARAAGVREVLVNDSHWDMRNLVWDELPEDEDLRVISGTRKPWSMAQGLDASF"
     gene            complement(291..722)
                     /locus_tag="VFU90_08445"
     CDS             complement(291..722)
                     /locus_tag="VFU90_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479845.1"
                     /translation="MSARRSTGLPPRIYLPILAVVAIAFLAIMAYLVAVGFGVTGSVF
                     GKAAKPSSAAAQAQGSQDAVEGGPPPAVAEQLKSLRERSAAHPNDDVALTQLGDMYLA
                     ANRFADAIPLYLRALRANPHNVAAQAGLSEARDALRGANAQ"
     gene            770..1333
                     /locus_tag="VFU90_08450"
     CDS             770..1333
                     /locus_tag="VFU90_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEU5479846.1"
                     /translation="MTTAILPTLAELQLLFARLNYQFFNGEVPDCRICYNERFSNSAG
                     RIDYAQRPVTIELSPKHFRRYPEALEETLLHEMIHAWCFARFRETGHGAAFKKKLRQC
                     GITSIYHDLGSVAPVKESAKRYILRCEHCAFEVLRKRKPGKPASCPRCNKRRFDPRYP
                     LTTYEIVETRRLGDTPAGRAARSLEAP"
     gene            complement(1330..1779)
                     /locus_tag="VFU90_08455"
     CDS             complement(1330..1779)
                     /locus_tag="VFU90_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479847.1"
                     /translation="MPLTIRRATSADTGAIAPLFDDYRRFFTQAPDLDVSTRFLRERL
                     EREESVVFAAFDGAEAAGFLQLYPLFSSWYARRQWFLSDLYVAPAFRERGIGKRLVEA
                     CAEYARQTHSRAILVELPYSEPHLTRFYGELGFAKDEIFELYRLALD"
     gene            1866..3296
                     /gene="fumC"
                     /locus_tag="VFU90_08460"
     CDS             1866..3296
                     /gene="fumC"
                     /locus_tag="VFU90_08460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU5479848.1"
                     /translation="MTKTDVSPNGARTRVESDSMGKIEVPADKYYGAQSARSLVHFDI
                     GDGSWPRDVMPKQVIVAMARLKKAAALVNGELGKLDREKTDLIVRAADEIVEGKLDEH
                     FPLRVWQTGSGTQTNMNVNEVISNRAIELAGGEMGSKKPIHPNDHVNMSQSSNDTFPT
                     AMHMAAAEAMSEMLPPVEALRDALDAKAKQWSHIVKVGRTHLQDATPLTLGQEFSGYV
                     TQLDRALAACREALDHLYDLAIGGTAVGTGLNAHPEFGERTAKKLAEITGLPFRSHPN
                     KFTALASHDDFVFASGALKMLAAALMKIANDIRWLASGPRAGIGELILPENEPGSSIM
                     PGKVNPTQSEAMTMVCVQVYGNDLAISFGASQGNFELNVFNPVMIYNFLHSTTLLRDA
                     CTMFREHAVEGLEANEEQIKQYLDQSLMVVTALSPHIGYDKAAEIAKHAHHHKSTLKE
                     AAIALGHVTEDQYDAIVVPKEMTRPK"
BASE COUNT          664 a         1127 c         1108 g          541 t
ORIGIN      
        1 aaagctcgca tcgagtccct gcgccatcga ccacggcttg cgcgttccgg aaatgacccg
       61 cagatcctcg tcctcgggca gctcgtccca cactaggttg cgcatgtccc agtgcgagtc
      121 gttcacgagc acctcgcgca cgccggccgc gcgggcgccg tcgatggccg cgcgcacctc
      181 gcgcgtcatg tagcggcggt agatcgggta ctcgtacgcg tcgctcgggt cgcactgctg
      241 ccaggcggag acgccggccg ttccctccat gtcgcacgag aggtagagct tcattgcgcg
      301 ttcgcgccgc gcagcgcgtc gcgcgcttcg gaaaggccgg cctgggcggc gacgttgtgc
      361 ggattggcgc gcagcgcgcg aagatacagc gggattgcgt cggcgaaacg gttggctgcg
      421 agatacatgt cgccgagctg cgtgagcgcg acgtcgtcgt tggggtgcgc ggcactccgc
      481 tcgcgcaaac ttttgagctg ttcggccacg gccggcggag ggccgccttc gaccgcgtct
      541 tgcgaacctt gcgcttgggc cgccgcggag ctcggtttgg ccgcttttcc gaacaccgaa
      601 ccggtaacgc cgaaaccgac ggcgaccaaa taggccataa tcgcgagaaa cgcgatggcg
      661 acgacggcca agatcggaag gtaaatgcgc ggcggaaggc cggtgcttcg acgggcggac
      721 acggaagagt cgtttggaca cgcggcgaag acgccctccg ccaccgcaca tgacgaccgc
      781 cattctgccc accctcgccg agctgcagct cctgttcgcc cggctgaact atcagttttt
      841 caacggcgaa gtgccggatt gccgcatttg ctacaacgag cgcttctcga attccgccgg
      901 ccgtatcgac tacgcgcagc ggcccgtcac catcgaactg tcgcccaagc actttcgccg
      961 ctaccccgag gcgctggaag aaactctgct ccacgagatg attcacgcgt ggtgcttcgc
     1021 gcgctttcgc gaaaccggcc acggcgccgc cttcaaaaag aaactgcgcc aatgcggcat
     1081 cacctcgatc tatcacgatc tcgggagcgt cgcgccggtc aaggagtcgg ccaagcggta
     1141 cattttgcgc tgcgaacact gcgcgttcga ggtgctgcgc aaacgcaagc ccggaaagcc
     1201 ggcgagctgc ccgcgctgca acaaacgccg cttcgatccc cgctatccgc tgacgaccta
     1261 cgaaatcgtc gaaacgcgcc ggctcggcga tacgccggcc ggccgggcgg cgcggtcgct
     1321 ggaagcgccc tagtctagcg cgaggcggta gagctcgaag atctcgtctt tggcgaaacc
     1381 gagctcgccg tagaagcgcg tcaggtgcgg ctcggaatac ggcagctcga cgagaatggc
     1441 gcgggagtgc gtttgcctag cgtactccgc gcacgcctcc accaatcgtt ttccgatgcc
     1501 gcgctcgcga aacgccggcg cgacgtagag atcgctgaga aaccactgcc ggcgcgcgta
     1561 ccacgacgag aacagagggt agagctgcaa gaatccggcg gcttcggcgc cgtcgaaggc
     1621 tgcgaagacc accgactcct cgcgctccag ccgctcgcgc aagaagcggg tcgaaacgtc
     1681 gaggtcgggc gcctgcgtga agaagcggcg gtagtcgtcg aacagcggtg cgatcgcacc
     1741 ggtgtcggcc gaggtcgctc ggcgaatcgt caacggcatc gccgccgctt ccgggcgttt
     1801 cggcccgctc cctgcgacct aggtccaggc agaaattcgg cattccgggc gaagataggc
     1861 cggcgatgac taagacggac gtttccccca acggggccag gacgcgcgtg gaaagcgact
     1921 cgatgggcaa gatcgaggtt cccgcggata agtattacgg cgcgcagtcg gcgcgctcgc
     1981 tcgttcactt cgacatcggc gacggcagct ggccgcgcga cgtgatgccg aagcaggtga
     2041 tcgttgcgat ggcgcgtctg aagaaggccg cggccctcgt gaacggcgag ctcggcaagc
     2101 tcgaccgcga gaagacggat ctgatcgtga gagccgccga cgagatcgtc gaaggcaaac
     2161 tcgacgagca cttcccgctg cgcgtgtggc aaaccggttc gggaacgcag acgaacatga
     2221 acgtcaacga agtcatttcg aaccgcgcga tcgagctggc cggcggggaa atgggctcga
     2281 agaagccgat ccacccgaac gatcacgtaa acatgtcgca gtcgtcgaac gatacgtttc
     2341 cgaccgcgat gcacatggcc gccgccgaag cgatgtcgga aatgctgccg ccggtcgagg
     2401 cgctgcgcga cgcgctcgac gcgaaggcga agcagtggtc gcatatcgtc aaggtcggcc
     2461 ggacgcatct tcaagatgca acgccgctca cgctcggcca agagttctcg ggatacgtta
     2521 cccagctgga tcgcgccctc gccgcctgcc gcgaggcgct ggatcatctg tacgatctgg
     2581 cgatcggggg gaccgcggtg gggaccggcc tcaacgccca tcccgagttc ggcgagcgca
     2641 ccgccaagaa gctcgccgag atcaccggtt tgccgttccg ctcgcatccc aataagttta
     2701 cggcgctggc gtcgcacgac gacttcgtgt tcgcatcggg cgccctgaag atgctggccg
     2761 ccgcgctgat gaagatcgcc aacgatatcc gctggctcgc ctcgggcccg cgcgcgggga
     2821 tcggcgagct gatccttccg gaaaacgagc cgggcagctc gattatgccc ggtaaagtga
     2881 acccgaccca gtccgaagcg atgacgatgg tatgcgtgca ggtttacggc aacgacttgg
     2941 cgatcagctt cggcgcgtcg caaggcaact tcgagctcaa cgtcttcaac ccggtaatga
     3001 tttataactt cctgcactcc acgacgttgc tgcgcgacgc ttgcacgatg ttccgcgaac
     3061 acgcggtcga aggcctggaa gccaacgaag agcagatcaa gcagtatctc gatcagtcgc
     3121 tgatggtcgt gacggccctt tcgccgcata tcggctacga caaggcggcc gaaatcgcca
     3181 agcacgcgca ccaccacaag agcacgctca aggaagcggc gatcgcgctc gggcacgtaa
     3241 ccgaagacca atacgatgcg atcgtcgtcc ccaaggagat gacccgcccg aagtagccgg
     3301 cgccctacaa cccgtcgacg acgaacgccg ccggtttgcc gcggtaggag ccgatgccgg
     3361 cgatcccgcc gccgggcgta aacgcgtagc cgcattcgaa gcgccatccg ggcgccgcga
     3421 ccacgtcgtc cagccgccgc
//
LOCUS       DASUCJ010000709         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_37980, whole genome shotgun sequence.
ACCESSION   DASUCJ010000709 DASUCJ010000000
VERSION     DASUCJ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37980"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1018
                     /locus_tag="VFU90_08465"
     CDS             272..1018
                     /locus_tag="VFU90_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5479849.1"
                     /translation="MIEHFQLRLADSRMLAPSVRHLAFERADGAPFAFTPGQFIQIHF
                     HYDDGKPTKRSYSVGTPAGEAGNVRRLELAVSYVEGGAATNLFDSLEAGGEIEASGPY
                     GRFCLMDDDVNRRYLLIATGTGITPYRAMLPRIEKLIREHGRRFVLLYGARNEQELLY
                     GEEFDAFAHQHPEFTFHPCLSRAARAAPRPNDRRGHVQDVLPELAPDPEHDIAYLCGN
                     PDMIDATFTQLKELGMPIKHIRREKYVSSR"
     gene            complement(1045..1413)
                     /locus_tag="VFU90_08470"
     CDS             complement(1045..1413)
                     /locus_tag="VFU90_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479850.1"
                     /translation="MRSIQQKHAFPLHDTSNVHASMRPRRYRRGDARQRDEVFEASEH
                     RGAGLPSHALQHPFEFQENGLRDEQVTAFQQVAREVRLRGVDLEVVAREDIGINRARS
                     AIFFAAARPFRSPTGIRGPE"
     gene            complement(1410..2048)
                     /locus_tag="VFU90_08475"
     CDS             complement(1410..2048)
                     /locus_tag="VFU90_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479851.1"
                     /translation="MADAVTAYYRGKGVDVRAGIKVEGRADDGRGLRLSDGSTLAADV
                     VVAGLGISPTVQLAEQAGLDVDNGIVVDDHLRSSDPDIYAAGDVASFPAPALGRRIRV
                     EHENAAISMGTHAGRCMAGEDAPYDELPFFYSDLFDLGYEAVGMLDSRLETVEQWVTP
                     FREGVVYYLDAGRVRGVLLWNTWGQVDAARNLIASPGPFDAENVLGRLPASA"
BASE COUNT          359 a          719 c          653 g          334 t
ORIGIN      
        1 gaagtcgcct tgcgccactt tcagcgaaac gcccttgagt gcctggacgc cgctggcata
       61 ggttttgcgc agatcgatga cttccagcgc cggaacggct cgggaaggat cggcggcagg
      121 catggaaggg gacgggttag gcaaagcgga atcggcagcc atcccctatt atagaagttt
      181 cgcccgcgcg acccgctgcc ctacggcagg ggcatcgcgg acccccaacc cacattattc
      241 ggcgaatgcc tgccgtcgcg cccaccgcac catgatcgaa catttccagc tacgactcgc
      301 cgacagccgc atgctcgcgc ccagcgtgcg ccacctcgcg ttcgagcgcg ccgacggcgc
      361 gccgttcgcg ttcacgccgg ggcagttcat ccagatccat ttccattacg acgacggcaa
      421 gcccaccaag cgcagctatt cggtcggcac gccggcaggc gaagccggca acgtgcggcg
      481 actggaacta gccgtctcct atgtcgaggg cggcgctgcg accaatctgt tcgacagcct
      541 cgaagcgggc ggcgagatcg aggcgagcgg cccgtacgga cgattctgcc tgatggacga
      601 cgacgtcaat cgtcgttatc tcctgatcgc gacgggcacc ggaatcacgc cctaccgcgc
      661 gatgctgcca cggatcgaaa aactcatccg cgaacatggc cggcgtttcg tgctcctcta
      721 cggcgcgcgc aacgaacagg agctactcta cggcgaggag ttcgacgcct tcgcgcacca
      781 gcacccggaa ttcactttcc acccctgcct gtcgcgcgcc gcacgcgccg cgccgcggcc
      841 caacgaccgc cgcggccacg tccaggacgt gctgccggaa ctcgcgcccg atcccgagca
      901 cgacatcgcg tacctgtgcg gcaaccccga catgatcgac gccaccttca cccagttgaa
      961 ggaactcggc atgccgatca agcacatccg gcgcgagaag tacgtttcct cgcgctagct
     1021 cggcagcggc ccggcgatcc tgggctattc cgggccacgt attccggtcg gcgatctgaa
     1081 cggccttgcc gcggcaaaaa agatcgcaga gcgtgcgcgg ttgataccga tatcctcgcg
     1141 cgccactacc tccaggtcga cgccgcgcag gcgaacctcg cgcgcgacct gttggaaggc
     1201 ggtgacttgt tcgtcccgaa gaccgttctc ctggaactcg aatgggtgct gcagggcgtg
     1261 gctgggcagc cccgcgccac ggtgctcgct tgcctcgaac acctcgtcgc gctgccgggc
     1321 atctccgcgg cgatatcgcc gcggcctcat cgaagcatgt acgttcgagg tgtcatgcag
     1381 cggaaacgcg tgtttctgct gtatggacct cacgcgctcg ccggcaatct tccgagcacg
     1441 ttctcggcgt cgaacggccc gggcgacgcg atcaggttgc gcgcggcatc gacttggccc
     1501 caggtattcc ataggagcac gccgcgtacg cggccagcgt cgaggtaata gaccacgcct
     1561 tcgcggaagg gcgtgaccca ttgttcgacg gtttccaggc gcgaatcgag catgccgacg
     1621 gcctcatagc caaggtcgaa gaggtccgaa tagaagaatg gcagctcgtc gtagggcgcg
     1681 tcctcgccgg ccatgcaccg gcccgcatgg gtgcccatgc tgatcgccgc gttttcgtgc
     1741 tcgacccgga tgcggcggcc cagcgcgggc gcgggaaagg acgccacgtc gcccgccgcg
     1801 tagatatcgg ggtcggagct gcgcaggtgg tcgtcgacca cgatgccgtt gtcgacgtcg
     1861 agaccggcct gctcggcaag ttgcacggtg ggcgagatgc cgagccccgc caccaccacg
     1921 tcggccgcca gcgtgctgcc gtcggagagg cgcaatccgc ggccgtcgtc cgcgcgtccc
     1981 tcgaccttga tgccggcgcg cacgtccacg cccttcccgc gatagtaggc cgtgaccgcg
     2041 tcggccagcg attcggggta gaccc
//
LOCUS       DASUCJ010000710         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_129946, whole genome shotgun sequence.
ACCESSION   DASUCJ010000710 DASUCJ010000000
VERSION     DASUCJ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129946"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFU90_08480"
     CDS             <1..517
                     /locus_tag="VFU90_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479852.1"
                     /translation="APNPPGNTVLPAVSWVIPDGVDSDHSNGGQDNGPDWVASVVNAI
                     GESSYWNSTAIIVVWDDWGGFYDHVPPPQLDSVGLGFRVPLIVLSPYAKTGYVSHTQY
                     EFGSILKFVESTFGLPSLGTTDVRANNLTDAFDFNQQPRKFVPIKTLNARHDRRYFLM
                     RPPSLEPVDDQ"
     gene            complement(524..1336)
                     /locus_tag="VFU90_08485"
     CDS             complement(524..1336)
                     /locus_tag="VFU90_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479853.1"
                     /translation="MDWLSRVSVLVALPAYALIFAGAAVLAHSAFRRFVPPERLREQY
                     DVAGFLVSVVGVLYSVVLGFLVGTVWTAFAAAQDTTNLEAGYVADAFNFARQLPQPPR
                     TELETLLARYAFEVRDIEPPILSARREDARAIVLINRAIDVIVRMPPPNTRNQGEILE
                     SSTIRDAVLGNLRNVGDQRRLRMLQAQSRLPPGMYEALILGASLVIAFAFFFGIRNYF
                     KQMAMTALVAASIGLFFGLVVELSTPYSGSIQVSRDAWTLIIDNNHMAAFIK"
BASE COUNT          290 a          518 c          502 g          276 t
ORIGIN      
        1 ggcgccgaac ccgcccggca acaccgttct gccggcggtc agctgggtga tccccgacgg
       61 ggtcgactcc gaccactcca acggagggca ggataacggt cccgactggg ttgcttcggt
      121 cgtcaatgcg atcggcgaga gctcctattg gaactccacc gccatcatcg tggtgtggga
      181 cgactggggc ggcttttacg atcacgtgcc gccgccgcag ctcgactccg tcggcctcgg
      241 cttccgggta ccgttgatcg tcctttcccc gtacgccaag accggatacg tctcccacac
      301 gcagtacgag ttcggcagca ttctgaaatt tgttgagtcg acgttcggtc tcccgagctt
      361 gggcaccacc gacgtgcgtg cgaacaacct caccgacgcg ttcgacttca atcagcagcc
      421 gcgcaagttc gtgcccatca agacgctgaa cgcgcgccac gaccgccgct acttcctcat
      481 gcggccgcct tcgctcgaac cggtcgacga ccagtaacgc tcgctacttt ataaaggccg
      541 ccatgtggtt gttgtcgatg atcagcgtcc acgcatcgcg cgacacctga atcgaacccg
      601 aatagggcgt gctcagctcg accaccaggc cgaagaataa gccgatcgag gcggcgacca
      661 atgcggtcat cgccatctgc ttgaaataat tccggattcc gaagaagaac gcgaaggcga
      721 tcaccagcga cgcgccgaga atcagcgctt cgtacatgcc cggtggaaga cggctctgcg
      781 cctggagcat tcgcagccgg cgctggtcgc cgacgttgcg cagatttccg agaaccgcat
      841 cccgaatcgt gctgctttcg aggatttcgc cctgatttct ggtgttgggc ggcggcattc
      901 taacgatgac gtcgatggcg cgattgatca gcacgatcgc gcgcgcgtcc tcgcgccgcg
      961 cgctgaggat cggcggctcg atgtcgcgca cttcgaaggc gtagcgcgcc aggagcgtct
     1021 cgagctcggt gcgcggcggc tgcggtaact gtctggcgaa gttgaacgcg tcggcgacgt
     1081 agccggcctc caagttggtg gtatcctgcg ccgccgcaaa ggccgtccaa accgtcccca
     1141 cgagaaagcc gagcacgacg gagtagagca cgccgaccac cgaaaccagg aagcccgcga
     1201 cgtcgtactg ttcgcgcagt cgctccggcg gaacgaagcg gcgaaacgcc gagtgggcga
     1261 gcaccgccgc gcctgcaaag atcagtgcgt aggcgggaag cgccaccaag acggaaaccc
     1321 gcgatagcca gtccatgtcg cgggggcgtt cgcgccgccg ctgcgcgcct ccgctcagga
     1381 cgctccgctc aggatgacag gaaagttcta tcgttgaatg cgagcgccgt ggtcagggtc
     1441 gaagtcgtcg tcccggagct ctggcacgcg ctcggcggcg tgatgcaact gcgaggcgac
     1501 gcttacgccg atgcggtcgc ttcccggccc ggcttcttgg tcgcgctggc gatcgtgctt
     1561 gccgccggat tgagcgaagc gctcgg
//
LOCUS       DASUCJ010000711         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1459566, whole genome shotgun sequence.
ACCESSION   DASUCJ010000711 DASUCJ010000000
VERSION     DASUCJ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459566"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFU90_08490"
     CDS             complement(<1..599)
                     /locus_tag="VFU90_08490"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU5479854.1"
                     /translation="MSDQRHSIAQAVSLEGIGLHLGRACRLTFRPAEAGTGVVFRRVD
                     LSGEPSIPARADFAVLSDRRTQLGSGPAALHTVEHVLAAVAAQDIDDLVIEMDGPEPP
                     ILDGSAAPYFDALGRAGIADRGGAVQYLAVTRPMSVKCGESMYEVLPAPSLSLKVTID
                     FPHPLIGRQTLDMGVTRDSFARELAGARTFGFTREVEMLS"
     gene            complement(596..1645)
                     /gene="lpxD"
                     /locus_tag="VFU90_08495"
     CDS             complement(596..1645)
                     /gene="lpxD"
                     /locus_tag="VFU90_08495"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU5479855.1"
                     /translation="MRLGDEREDGPSLTAEAIARLVGGTIRGDGGARVRGVAPLDRAT
                     TDDLSFLAQPRYAALFERSQAGVVLVAPELADAPGAAACRIVVRQPHEAMLRVLPELY
                     PPPPRESGVHGTAVIGRGARLGDGVAIGPYAVIGEGVQVGDDAVIGAHATIGAGVTVG
                     ARTWLADGVTLYAGTVLGERVRVHAGARIGCDGFGYVPGEDGHRKIPHVGRCIIGDDV
                     EIGANSTIDRGSVDDTVIGAGTKIDNLVHIAHNVRIGRLCLIMAQVGIAGSVHMGDGC
                     ILAGQVGVSGHHDIGAGARLAAQAGVFGDIPAGQTWSGYPARPHGEQLRAHAALYKLS
                     GLMRRIEKMLERDTG"
     gene            complement(1666..2319)
                     /locus_tag="VFU90_08500"
     CDS             complement(1666..2319)
                     /locus_tag="VFU90_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5479856.1"
                     /translation="MRSFVHAALSALAISTLVAGTALAQGGTVSPPASSSSGTIKIAY
                     VNSQKILAEVPGRTDAEAQMQKEMEGYRAEVQKMSDSLNAIVSAYNKAEPTLSATAKA
                     TRQKDIQTKESAYQQRVQQLEQQAQQRQAELMRPIMQQINRVIEQIRSENGYAMVFDA
                     GNQSGVVVAADSALDITDKVIQRLKAAGPVSTTPQKPPAGPTDKPLGASRSPDPRKP"
     gene            complement(2331..>2880)
                     /locus_tag="VFU90_08505"
     CDS             complement(2331..>2880)
                     /locus_tag="VFU90_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5479857.1"
                     /translation="SSFQRYTTELRNYSTLATWGGQQIGSQSMKLVFGLTARAGMLFG
                     NAGPFFYSQKFAMGGTQYGEQLRGYEEFSITPAGFVPGTNSNATVQSFGNAFFSTTAE
                     LGVRFNSMLYSDLFYDAGNVYDSPRDFDPTRLFRGAGIGVAIVTPLGPLGLDWAYGFD
                     RVDITGRPDPKWMLHFKLGQIF"
BASE COUNT          471 a         1063 c          870 g          476 t
ORIGIN      
        1 gacagcatct cgacctcgcg cgtgaatccg aacgtccgcg cgccggccag ctcccgcgcg
       61 aacgaatcgc gcgtgacgcc catgtccaac gtctgccggc cgatcagcgg atgcggaaaa
      121 tcgatcgtca ccttgagcga caacgacggc gccggcagca cctcgtacat cgactcgccg
      181 cacttcaccg acatagggcg cgtcacggcg agatattgca ccgccccacc gcgatcggca
      241 atccccgccc tgcctaacgc atcgaagtac ggcgccgcgc tgccgtccag gatcggcggc
      301 tccggcccgt ccatctcgat cacgaggtcg tcgatgtcct gcgccgccac ggccgccaac
      361 acgtgctcca ccgtgtgcag cgccgccggc ccgctgccga gctgcgtccg ccggtcgctc
      421 agcaccgcaa aatccgcccg cgccggaatg ctcggttccc ccgagagatc cacgcgacgg
      481 aagaccacgc ccgtgccagc ctccgccggc cggaacgtga gacggcacgc cctcccgaga
      541 tgcagtccga tcccctcgag cgacaccgcc tgcgcaatcg aatggcgctg gtcgctcacc
      601 cggtgtcgcg ctccaacatt ttctcgatgc gccgcatgag ccccgacaac ttgtacagcg
      661 cggcgtgcgc gcgcagttgc tcgccgtgcg gacgcgccgg atagcccgac caggtctgcc
      721 ccgcgggaat gtcgccgaac accccggcct gcgccgccag gcgggccccg gcgccgatgt
      781 catgatgccc gctcaccccc acctgccccg cgagaatgca cccgtcgccc atgtgcaccg
      841 atcccgcgat gcccacctgc gccatgatca gacagagccg gccgatgcgc acgttgtgcg
      901 cgatgtgcac gaggttgtcg atcttcgttc cggcgccgat cacggtatca tcgacgctcc
      961 cgcggtcgat ggtcgagttg gcgccgatct ccacatcgtc gccgatgatg caccggccga
     1021 cgtgcggaat cttgcgatgg ccatcctcgc ccggcacgta gccgaacccg tcgcacccga
     1081 tgcgcgcgcc cgcgtgcacc cgcacgcgtt cgcctaacac cgtgcccgcg tacagcgtca
     1141 cgccgtcggc cagccaggtg cgcgcgccca ccgtcacgcc ggctccgatc gtcgcgtggg
     1201 cgccgattac cgcgtcgtcg cccacctgca ctccctcgcc gatcaccgcg tacgggccga
     1261 tcgccacgcc gtcgcctaac cgagcgccgc ggccgatgac cgccgtgccg tgcacgccgg
     1321 actcgcgcgg cggcggcgga tagagttccg gcaacacacg cagcatcgcc tcgtgcggct
     1381 gccgcaccac gatccggcac gccgcggcac ccggcgcatc ggcgagctcc ggcgccacca
     1441 gcacgacgcc ggcctgcgac cgctcgaaca gcgccgcgta ccgcggctgc gcgaggaagc
     1501 tcagatcgtc cgtcgtcgcc cggtccaacg gcgccacacc gcgcacgcgc gccccgccgt
     1561 ccccccgtat cgtgccgcct accaaacgcg caatcgcctc ggcggtgagt gaaggaccat
     1621 cttctctctc atcgccgagg cgcatgtgcg tcacgtcgtt gggcattacg gcttgcgggg
     1681 atccggcgat cgcgatgcgc cgagcggctt gtccgtgggt ccggcgggcg gcttctgcgg
     1741 cgtcgtcgac accggaccgg ctgccttgag tcgctggatc accttgtccg tgatgtcgag
     1801 cgccgagtcg gccgcgacca ccacgcccga ctgattcccg gcgtcgaaca ccatcgcgta
     1861 gccgttctcg ctccgaatct gctcgatcac cctgttgatc tgttgcatga tcggacgcat
     1921 gagctcggcc tgccgctgct gcgcctgctg ctcgagttgc tgcacgcgct gctggtacgc
     1981 actctccttc gtctgaatgt ccttctgccg cgtcgccttc gccgtggcac tcagcgtcgg
     2041 ctcagctttg ttgtacgccg acacgatggc gttgagcgaa tcgctcatct tctgcacttc
     2101 ggcgcgatag ccctccatct ccttctgcat ctgcgcttcg gcatcggtgc ggccgggcac
     2161 ctcggccaga atcttttgcg agttgacgta ggcgatcttg atcgtgccgc tcgacgagct
     2221 cgccggcggc gacacggtgc ctccctgcgc caacgctgtt ccagccacga gggtggagat
     2281 tgccagcgcc gagagcgcgg catgaacaaa cgaacgcatt gtgactccgg ttagaaaatt
     2341 tgtccgagct tgaagtgcag catccacttc gggtccgggc gcccggtgat gtcgacgcgg
     2401 tcgaacccat acgcccaatc gaggccaagc ggcccgagcg gcgtcacgat cgcgacaccg
     2461 atgccggcgc cccggaataa ccgcgtcgga tcgaaatcgc gcggcgaatc gtacacgttg
     2521 cccgcgtcgt aaaagaggtc cgagtacaac atggaattga agcgaacgcc gagctcggcc
     2581 gtcgtgctga aaaatgcgtt tccgaaggat tggacggtgg cgttgctgtt cgttcccggt
     2641 acgaaccccg ccggcgtgat tgaaaattcc tcgtaaccgc gcagttgttc gccgtattgc
     2701 gtaccgccca tggcgaactt ctgagaatag aagaaaggtc cagcgttgcc aaacagcatg
     2761 ccagctcgcg ccgtgagccc gaacacaagc ttcatggact gtgagccgat ctgctggccg
     2821 ccccacgtag ccaacgtgga gtagttacgc aactcggtag tgtaccgctg gaagctggac
//
LOCUS       DASUCJ010000712         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_22820, whole genome shotgun sequence.
ACCESSION   DASUCJ010000712 DASUCJ010000000
VERSION     DASUCJ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22820"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..694
                     /locus_tag="VFU90_08510"
     CDS             317..694
                     /locus_tag="VFU90_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5479858.1"
                     /translation="MDSATRAQLWAARDSVWRAWFAGDSASLSHLLPAAVAAGSGGGE
                     GWETRNATMAAARQFADGGGKLTALRFDSTSIRLRGNVAVMQSRYILTLEQRGERATR
                     TGIATEVFVRTPGGWQNPFWYLE"
     gene            complement(698..>1008)
                     /locus_tag="VFU90_08515"
     CDS             complement(698..>1008)
                     /locus_tag="VFU90_08515"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5479859.1"
                     /translation="YAFGADSIPAVSASASRDRRGVVHITLSNLDPNGPRTVVAELHG
                     LSATSASGRILSAPAIDSYNSFERPDVVRPAAFTDAHVENGRLTVTLPPRSVVELELR
                     "
BASE COUNT          151 a          309 c          360 g          188 t
ORIGIN      
        1 agcggcatcg cggcggctcg acgatggcgc ttctcgccag cggggcaaga gtgagcgtgt
       61 ctcgacgccg gcgagcgata ttcgcggccg cgctcgagca acgcggatga cgactccgca
      121 tcgacgtcgc cgcagccgtt cgccgcgatg gatcgaccgc tcacggaacc gtgattgcgc
      181 cgagcggcgg tgctggctgg attagtggcg ttcgtgagcg gagtctctca tgcgtgctct
      241 tatgttggtc gggttcgtcg ccgccctggc cagtcgaact gcgtgggcgc aggacaccgc
      301 gctccccgtg cagtccgtgg actcggccac gcgcgctcaa ttgtgggcgg cgcgagattc
      361 ggtgtggcga gcgtggtttg ccggcgattc ggcgtcgctc tctcatcttc tccccgccgc
      421 cgtggctgcc ggctctggcg gaggggaagg ctgggagact cgtaatgcaa cgatggccgc
      481 ggcgcgtcaa tttgccgacg ggggcggtaa gctgaccgcc ttgcgatttg acagcaccag
      541 cattcgactt cgtggcaacg tcgcggtcat gcagtcgcgg tatatcctga ctctcgagca
      601 gcgtggcgag cgggcgacac gaactggcat cgcgacggaa gtgttcgtgc gcactccggg
      661 cggatggcag aacccatttt ggtatctcga atgatcgtca tcgcagctcg agttcgacga
      721 ccgatcttgg cggcagcgtg accgtcagcc ggccgttctc gacgtgtgcg tcggtgaacg
      781 ctgccgggcg cacgacatcg ggccgctcga acgagttgta gctgtcgatg gcgggggcgc
      841 tgagaatgcg tccgctcgcg ctggtcgcac tgagaccgtg gagctccgcg acaacggtcc
      901 gtggcccatt cggatcgagg ttgctcaacg tgatgtgcac cacgccgcga cggtctcgcg
      961 acgcgctggc gctcacggcc ggaatggagt cggcgccgaa cgcatagt
//
LOCUS       DASUCJ010000713         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1276191, whole genome shotgun sequence.
ACCESSION   DASUCJ010000713 DASUCJ010000000
VERSION     DASUCJ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276191"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3709)
                     /locus_tag="VFU90_08520"
     CDS             complement(<1..3709)
                     /locus_tag="VFU90_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEU5479860.1"
                     /translation="MAKRTAILQAHEFFRGGPASAEGWSQLIGRGREWESFYRDRWQH
                     DKVVRSTHGVNCTGGCSWKIYVKGGVVTWETQQTDYPRTRAGMPNHEPRGCSRGASYS
                     WYLYSANRLKYPMVRGRLVRLWREARRTLAPVEAWASIVENPDHAREYKSIRGRGGFV
                     RVTWDEAQEIVAAANVYTIRKYGPDRICGFSPIPAMSMVSHASGARYLSLIGGSLLSF
                     YDWYCDLPPSSPQVWGEQTDVPESADWYNSQYLVAWGSNVPQTRTPDAHFFVEARYNG
                     TRVVSITPDYAEVAKFGDLWLHPKQGTDAALGMAMGHVILKEYYVDRTVPYFDDYARR
                     LTDLPLLVMLEKQGDRWIAGRFLRASDFAGALGHENSPEWKTIALDEASGAPVVPQGS
                     IGYRWGSRVEHDLGKWNLEQKNGATGADVKLALTLLGRHDDTLPVTFPYFGGTLHEHF
                     PANDQGSDVLVRKVPAKRLATVAGEALVTTVFDLMCANYGVDRGFGDGTAKSFDDDVP
                     YTPKWQQSITGVDPAHAIQVAREFASTAERTHGRSQIIIGAGMNHWYHQDMNYRAAIN
                     IAMLCGGVGVSGGGWAHYVGQEKLRPQTGWQVLAFATDWVRPARQMNGTSFFYAHSDQ
                     WRYEKIEVSSLLSPTADRARYPGSMIDFNVRAERMGWLPSAPQLAANPLLVAKDAVTA
                     GVDPREYVAKGLADGRLKLACEDPDNPINYPRNLFLWRSNLLGSSGKGHEYFLRHLMG
                     AQHSVLGPDLGEAGAAKPQEVVWHDAAPEGKLDLLTVLDFRMSTSCLYADVVLPTATW
                     YEKNDLNTTDMHPFIHPLSAAVDPAWEARNDWEIYKGIARKFSELCEGHLGVERDVVL
                     QPVMHDSPGEIAQPYDVADWKRGECELVPGRTAPNIFVVERDYPNTYKRYTALGPLIA
                     KLGNGGKGITWPMEKTVAGLGALNHLVEEEGVTRGLPRIDSDIDAAEVILYLAPETNG
                     DVAVHAWDALSKQTGRSHRHLAETREDEKIRYRDIQAQPRKIITSPTWSGIESEKVSY
                     TAGYTNVNELIPWRTLTGRQQFYQDHRWMRDFGEGLCLYKPPVDTKTVASVVRKSNGN
                     PEIVLNFITPHQKWGIHSTYTDNLLMLTLSRGGPIVWISETDARKAGIEDNDWIELYN
                     ANGALTARAVVSQRVQEGMCLMYHAQEKIVNLPGSEVTGHRGGIHNSVTRTLLKPTHM
                     IGGYAQLSYGFNYYGPTGSQRDELVVIRRRSQ"
BASE COUNT          596 a         1284 c         1231 g          665 t
ORIGIN      
        1 cctgggagcg gcggcggatg accaccagct cgtcgcgctg agatccggtg gggccgtagt
       61 agttgaaccc gtaggagagc tgggcgtagc cgccgatcat gtgggtcggc ttgagcagcg
      121 tgcgcgtgac cgagttgtgg atgccgccgc gatgcccggt cacttccgag cccggcagat
      181 tcacgatctt ctcttgcgcg tggtacatca ggcacatccc ttcctgcacg cgctgactca
      241 ccaccgctcg cgcggtcagc gcgccgttcg cgttgtagag ctcgatccag tcgttgtcct
      301 cgatgccggc cttcctcgcg tcggtctccg agatccacac gatcggcccg ccacgcgaca
      361 gcgtcagcat caggaggttg tcggtgtagg tcgagtgaat gccccacttc tggtgcggcg
      421 tgatgaagtt cagcacgatc tcgggattgc cgttcgactt gcgcacgacg gaggcgacgg
      481 tcttcgtgtc gaccggcggc ttgtagaggc agaggccttc gccgaagtcg cgcatccagc
      541 ggtgatcctg gtagaactgc tggcgcccgg tgagcgtccg ccagggaatc agctcgttga
      601 cattggtgta gcctgccgta tagctcacct tctcgctctc gatgcccgac caggtcggcg
      661 acgtgatgat cttgcgcggc tgcgcctgga tgtcgcggta gcggatcttc tcgtcctcgc
      721 gcgtctcggc aaggtgccga tgcgagcgac cggtctgctt cgacagcgcg tcccacgcgt
      781 gcacggcaac gtcgccgttg gtctccggag cgagatagag gatcacctcg gcggcgtcga
      841 tgtcgctatc gatccgcggc agacccctgg tgactccctc ctcctcgaca aggtggttga
      901 gcgcaccgag cccggcgacc gtcttctcca tcggccaggt gatgccctta ccgccgttgc
      961 cgagcttcgc gatcagtggc cccagcgccg tatagcgctt gtaggtattc gggtagtcgc
     1021 gctcgacgac gaagatgttg ggcgccgtgc ggcccggaac gagctcgcac tcgccgcgct
     1081 tccagtcggc gacgtcgtac ggctgcgcga tctccccggg cgaatcgtgc atgaccggct
     1141 gcagcacgac gtcgcgctcg acgccgagat gcccttcgca gagttccgag aacttccgcg
     1201 cgatcccctt gtagatctcc cagtcattgc gcgcctccca cgccgggtcg acggccgccg
     1261 acagcggatg gatgaacggg tgcatgtcgg tcgtgttgag gtcgttcttc tcgtaccacg
     1321 tcgccgtcgg cagcacgacg tcggcataga gacaactcgt cgacatgcgg aaatcgagca
     1381 cggtgaggag atcgagcttc ccctcgggcg ctgcgtcgtg ccaaacgacc tcctgcggct
     1441 tcgccgctcc ggcctcgccg agatcggggc cgagcacgct gtgctgcgcc cccatcagat
     1501 gccgcagaaa gtattcgtgc cccttcccgg acgatccgag gaggttggag cgccacagga
     1561 acaggttgcg cggatagttg atcggattgt cgggatcctc gcaggcgagc ttcagtcgcc
     1621 cgtcggcgag ccccttcgcg acgtactcgc gcgggtccac gcccgcggtg acggcgtcct
     1681 tcgcgaccag aagaggattc gccgcgagct gcggcgccga cggcagccaa cccatccgct
     1741 cggcgcgcac gttgaagtcg atcatcgaac ccggatatcg ggcgcgatcc gcggtcggtg
     1801 acaagaggct cgacacctcg atcttctcgt accgccactg gtcggaatgc gcatagaaga
     1861 acgacgttcc gttcatctgc cgcgccgggc gcacccagtc ggtcgcgaac gcgagcacct
     1921 gccagccggt ctgcgggcgc agcttctcct gcccgacgta atgcgcccag ccgccgcccg
     1981 agacgccgac gccgccgcag agcatcgcga tgttgatcgc ggcgcgatag ttcatgtcct
     2041 ggtggtacca gtggttcatc ccggcgccga tgatgatctg cgagcgcccg tgcgtgcgct
     2101 cggccgtcga cgcgaactcg cgcgccacct ggatcgcgtg cgccggatcg acgccggtga
     2161 tcgactgctg ccacttcggc gtatacggga catcgtcgtc gaacgacttc gcagtgccgt
     2221 cgccgaaacc gcgatcgacg ccgtagttcg cgcacatcag atcgaacacg gtagtcacga
     2281 gcgcctctcc ggcgaccgtc gcgaggcgct tcgccggaac cttgcgcacg agcacgtcgc
     2341 ttccctgatc gttggccggg aagtgctcgt gcagcgtgcc gccaaaatag gggaaggtga
     2401 ccggcagcgt gtcgtcgtgg cggccgagga gcgtcagcgc gagcttcacg tccgctccgg
     2461 tcgccccatt cttctgctcg aggttccact tgccgagatc gtgctcgacg cggcttcccc
     2521 agcgatagcc gatcgaaccc tgcggcacga ccggcgcgcc gctcgcctcg tcgagcgcga
     2581 ttgtcttcca ctcggggctg ttctcgtgcc cgagcgcacc ggcaaaatcg gaagcacgca
     2641 gaaagcgccc ggcgatccag cgatcgccct gcttctccag catcacgagc agcggcaggt
     2701 cggtcaagcg ccgcgcgtag tcgtcgaagt acggcaccgt gcggtcgacg tagtactcct
     2761 tcaggatcac gtgccccatc gccataccga gcgcggcatc ggtgccctgc ttcgggtgga
     2821 gccagaggtc gccgaacttg gccacttcgg cgtaatcggg ggtgatcgac acgacgcgcg
     2881 taccgttgta gcgcgcctcg acgaagaagt gcgcgtcggg agtgcgcgtc tgcgggacgt
     2941 tcgagcccca cgcgacgagg tactgcgagt tgtaccagtc ggccgactcg ggcacgtcgg
     3001 tctgttcgcc ccacacctgc ggcgacgacg gcggcaagtc gcagtaccag tcgtaaaacg
     3061 acaagagcga gccgccgatc agcgacaggt aacgcgctcc cgatgcgtgc gagaccatcg
     3121 acatcgccgg aatcggcgag aagccgcaga tgcggtcggg gccgtacttg cggatcgtgt
     3181 agacgttcgc cgccgcgacg atctcctgcg cctcgtccca ggtgacgcga acgaatccgc
     3241 cgcggccgcg gatcgacttg tactcgcgcg cgtgatccgg attctcgacg atcgacgccc
     3301 acgcctcgac cggcgccagc gtccgccgcg cctcgcgcca caggcgcacg agacgcccgc
     3361 gcaccatcgg gtacttgagc cggttggcgc tgtacaggta ccaggaatac gacgcgccgc
     3421 gcgaacagcc gcgcggttcg tgattgggca tgccggcacg cgtgcgcggg taatcggtct
     3481 gctgcgtctc ccaggtgacc acgccgccct tgacgtagat cttccaactg cacccgccgg
     3541 tgcagttgac gccgtgcgtc gagcgcacga ccttgtcgtg ctgccagcgg tcgcggtaga
     3601 acgactccca ctcgcgcccg cggccgatca gctggctcca cccctcggcg gaggcggggc
     3661 cgccccggaa gaactcgtgc gcctggagga tcgcggttcg cttggccatg cccgcgcgtc
     3721 gattcttatc ggaccacgat cgtttcgggg tgcgccggca tcccagccat gtctcc
//
LOCUS       DASUCJ010000714         8968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_802632, whole genome shotgun sequence.
ACCESSION   DASUCJ010000714 DASUCJ010000000
VERSION     DASUCJ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802632"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="groEL"
                     /locus_tag="VFU90_08525"
     CDS             <1..836
                     /gene="groEL"
                     /locus_tag="VFU90_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5479861.1"
                     /translation="TAVAVKAPGFGDRRKRMLEDIAILTNGEVITEEMGLKLENTQLS
                     QLGRARRVVVAKDTTTIVDGAGDPPSIKGRINQIKGEIENTDSDFDREKLQERLAKLS
                     GGVAVVKVGAATETEMKEKKHRVEDALQATRAALEEGIVPGGGVALLQASAAISVSEN
                     GASADGSDDERTGARIVLRALEEPLRQIAANAGLEGSVVVNDVRKAKKGQGLNAATGE
                     IVDLVAEGVIDPAMVTRSALQNAASIAKNILTTEAIVAELPEKEGAGGGGGMPDMSGM
                     M"
     gene            1037..1441
                     /locus_tag="VFU90_08530"
     CDS             1037..1441
                     /locus_tag="VFU90_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479862.1"
                     /translation="MRQMLRSRPLESAEIDVGGKRLCVPTAAEILRIKAWLALTRNQT
                     RDYLDIAALADRIGLDEAAAVLGGIDEYYADINGRPEAVATQLVRQLAEPRPRDVEVT
                     AQLSSYKALDERWQDWSTVTAVLADLAERMLR"
     gene            1438..1905
                     /locus_tag="VFU90_08535"
     CDS             1438..1905
                     /locus_tag="VFU90_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5479863.1"
                     /translation="MTTQFRNVDFDRREPLDDWPAEAIATVIDRGSLSDWRRLAEAIR
                     RNPWGSAARTTETVVGWGEHYGVDALMSRLIAHAREQVNRRGRIAYAEQIRAWREQTG
                     MTLRQFARAAGTSASRLSDYENAKVAPTTDVLGRFRHVANEHRSGRKAAAHDA"
     gene            complement(1933..2373)
                     /locus_tag="VFU90_08540"
     CDS             complement(1933..2373)
                     /locus_tag="VFU90_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5479864.1"
                     /translation="MKLLDVNVVLAAHRDDHPDFELARAWLDYTLAERTPFAVVDLVA
                     GAFLRIATNRRIFAIPTPVDEAFAYLKALRAQPAHVMLAPGPPHLTLLERLCANGEAS
                     GDLVADAQLAAIALEHACELVSFDRDFARFSELRWSRPTGQEVS"
     gene            complement(2370..2606)
                     /locus_tag="VFU90_08545"
     CDS             complement(2370..2606)
                     /locus_tag="VFU90_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479865.1"
                     /translation="MDDHLLEAAKRRARERGETLGQVVEGALRRELAEPAAHEPVEVP
                     VFRGGNGPPAGVNLRSNSALRELLDRDAAMTKLR"
     gene            2879..3745
                     /locus_tag="VFU90_08550"
     CDS             2879..3745
                     /locus_tag="VFU90_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479866.1"
                     /translation="MLALATAAVALLGVLGMTSSAYAVTHHPKGEFAPFADCPLSNPA
                     AAQCLVAKTESGEIIVGKEDVPITNAITLQGGIEFTEAGEKLIAAEDGNTLSKSPQKV
                     PGGLSGLVKCNEISNFIERIACEVVFENGLTGVTATTELAAPASSVKIDQGALIFQSG
                     TALSLPVKLHLENPLLGSSCYVGSNAHPINIELTTGTTSPPAPNKPITGSPGEIGFNE
                     AETLITLKNNVLVNNSFPAPVAEGCGGLFAFLIDPIIDSRLGLPSAAGHNTAILRGTL
                     KAATAEAVKASE"
     gene            3933..4508
                     /locus_tag="VFU90_08555"
     CDS             3933..4508
                     /locus_tag="VFU90_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479867.1"
                     /translation="MLARSPHMRSPAAGLPAAVVLAVAALGACGCSGNTATTSTSASS
                     ATSTSRVSTSRVATSRPAAGGDSATFIARADAVCRRLNGQLAATAPHQGEAPAALARN
                     ALQHAALERRTSSELAKLPPPRALARDWARIVADRRTLAADLVTLARALSSGQSPQTI
                     GVLALKSATHKAMSAIAEHDGFADCAKVGSA"
     gene            4560..5414
                     /locus_tag="VFU90_08560"
     CDS             4560..5414
                     /locus_tag="VFU90_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479868.1"
                     /translation="METLVGDKDTFRLTRRRTPWQTGRAVDPRQSGDLAPEAGDRYRA
                     VAGSTRLAGDCRNKPSRRDKERFTMKRFKLLGLALVAVFALAAAFSASAFALPELLGQ
                     GANATYEGKNTAANPTLETTKSEKIVCKEATANGVQETNSLASFHISFKGCESSGFKC
                     NSSGDETGVILVLGSAHYVDSTPGNVAILFLFEELTIKCTALVTLKVKGDIVCPVLEA
                     LTSAATHEFHCTESAGKPSTTSWLNDENETQTAKVETSKNGGAFIESAEQALASVTFG
                     TAVAVMNE"
     gene            5868..6557
                     /locus_tag="VFU90_08565"
     CDS             5868..6557
                     /locus_tag="VFU90_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479869.1"
                     /translation="MSPASALAVAALVLAMTGGAYAAGRYVITSTKQIKPSVLKALKG
                     NAGPAGKAGAAGPAGSAGSQGAEGKQGPEGKQGPEGKAGPEGKSGEKGATGSPWTAGG
                     TLPKGATETGTWFAGVNSEKLGFDAISFSIPLAGALDAEHVHYVTIKEVEAAKAPAAC
                     PGSAAAPAARSGSFCVYEGALSNPQGGVEEAIIEDATAATAGAATTGAALVVVAKATE
                     STFWGSWAVTG"
     gene            6600..>8968
                     /locus_tag="VFU90_08570"
     CDS             6600..>8968
                     /locus_tag="VFU90_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479870.1"
                     /translation="MQPTTARSLMRRCAAAGGLALCLLAGLLGILSSPASAFKTRPLL
                     GKFGALSNPHGIAIDQSTGDLYVADTANNRIEKFDETGKFLLAFGANVGGAGVNTCTS
                     GCAAGTSGSAPGQFTTATFVAVDNSASASKGDVYVGDVGDNKVSKFTSSGELVSSWGT
                     GGQLDGSTTGFKTFAPLAGVAVDSSGNLFVLKGQTEPGRLFKFTQSGSFVTEEFGHSF
                     LRLNATLGLALDATGNIFKVNGDGSVDKLKSNLQEIGTVTPQKPAAKTRALDVDPATG
                     DLFLPTSAGTLDHYAFNASDQVIEPNGPPCPLVEAVGCTPSDSASIGFVGAGIAVNAA
                     SEDTYVSNPTTGQVFEYGPLVTVPDVTTEAATNVVSTAARFNGKVNPDELAVTTCEFE
                     YVSDAQLVELNGKDYEERIESGSSPAEIFEAVGSHAACEHPDAAEIGAGASNVAVHAD
                     VTGLTSGARYHFRLVAANANGPNAGSVLSFVTVTPPTIEGAEARDLTPTTANLEAEIN
                     PGSGETTYHIEYGTSTSYGTVVPVPDGDVGSGASDVPVAQHVAGLISGVAYHWRVVAS
                     NSAGTTIGADHVFIFKTIAATLPDGRAYEMVTPPQKNGSLIGDTGGLGLQPSIAAGGG
                     RLIATSLQCFAGAQSCNAQHGVGIGSPYSFERTSTAWITTPLAPPATGESPANTPWIY
                     LADNGSALFSMPTPPFGVDSLFKREAGGGFVAIGPISPSELGPQTPVAGKPGAFVQAQ
                     TADLSHIAWSSGSGPLHWPFDATEVKHSSIYEYTGTGNARPFLVGVTGGA"
BASE COUNT         1659 a         3027 c         3020 g         1262 t
ORIGIN      
        1 tcaccgccgt cgccgtcaag gcgccgggct tcggcgaccg ccgcaagcgc atgctcgagg
       61 acatcgcgat cctcaccaac ggcgaggtga tcaccgagga gatggggctg aagctggaga
      121 acacccagct atcccagctc gggcgagccc gccgtgtggt cgtggccaag gacaccacca
      181 cgatcgtcga cggcgccggt gacccaccgt cgatcaaggg ccggatcaac cagatcaaag
      241 gcgaaatcga gaacaccgac tcggacttcg accgcgagaa gctccaggag cgcctggcca
      301 agctctccgg cggcgtcgcg gttgtcaagg tcggggctgc gaccgagacg gagatgaagg
      361 agaagaagca ccgcgtcgag gacgcgctgc aggccacacg cgcggcgctc gaggagggca
      421 tcgtgccggg cggcggcgtc gccctgctgc aggcctccgc ggccatcagc gtctccgaga
      481 acggcgcgtc ggccgacggc tcggacgacg agcgcacggg cgcgcggatc gtcctgcgcg
      541 cgctcgagga gccgctgcgc cagatcgcgg cgaacgccgg cctggagggt tcggtcgtcg
      601 tcaacgacgt gcgcaaggcg aagaagggcc agggcctcaa cgccgcgacc ggcgagatcg
      661 tcgacctcgt cgccgaaggc gtcatcgacc cggcgatggt cacgcgctcg gcgctgcaga
      721 acgccgcgtc gatcgccaag aacatcctca cgaccgaggc gatcgtcgcc gagctgcccg
      781 aaaaggaagg cgccggcggc ggtggtggga tgcccgacat gtcggggatg atgtagagcc
      841 tcccttgcag ggaaagtccg accgtttctt ctggtcgatg cgagggcccg gcgactgccg
      901 ggccctcgtg ctggtgcgcc atgcacgact gcgtgccggg gtgacgttat cggattagat
      961 aacatgcgcg atatggatcc ctcgctcgag gaagtcctcg agaaggctgc gtagctgcgg
     1021 gggcatcgag acggggttgc gtcagatgct tcgaagccgg ccgctcgaga gcgccgagat
     1081 cgacgttgga ggcaagaggc tctgcgtgcc cacagcggct gagatcttgc ggatcaaggc
     1141 ctggctcgcg ctcactcgca accagacgcg tgactacctc gacattgctg cgctcgccga
     1201 ccggatcggg ctcgacgagg cggccgccgt gttgggaggc atcgacgagt actacgcgga
     1261 tatcaacggc cggccagagg cggtggcgac gcagttggtc aggcagttgg ccgagcctcg
     1321 tccgcgcgac gtcgaggtca ctgcgcagct ttcgagctac aaggcattgg acgagcgctg
     1381 gcaggactgg tcgaccgtca ccgcggtgct cgcggacctg gcggagagga tgctgcgatg
     1441 acgacacagt ttcgaaacgt cgatttcgac cgccgcgagc cgctggacga ttggccggcc
     1501 gaggcgattg cgacggtcat cgaccgtggt tcgctttccg attggcggcg gcttgccgag
     1561 gcgatccggc gcaacccgtg gggctcggcg gcgcgtacga ccgagacggt cgtcggatgg
     1621 ggcgagcact atggggttga cgcgctcatg tccaggctga tcgcgcacgc gcgcgagcag
     1681 gtcaatcgac gtggacggat tgcatacgcc gagcagatcc gcgcgtggcg cgagcagaca
     1741 ggcatgacgt tgcggcagtt cgcccgggcc gcaggcacct cggcgtcgcg gctgtcggac
     1801 tacgagaacg cgaaggtcgc cccgaccaca gacgtgcttg gccggttcag gcatgtagcg
     1861 aatgagcacc gcagcggccg gaaggcggcg gcacacgatg cgtgaacggc gggtcgacgg
     1921 cggcgatcag gatcaactga cctcttgccc ggttgggcgg ctccatctca gctcggagaa
     1981 gcgggcgaag tcgcggtcga aggaaaccag ctcgcaggcg tgctcgagcg cgatcgcggc
     2041 gagctgggcg tccgcgacga gatcgcccga ggcttcgccg ttggcgcaca gccgctccag
     2101 gagagtgagg tgcggcggcc cgggcgcgag catgacgtgc gcgggctggg cgcgtagcgc
     2161 cttcaggtag gcgaacgcct cgtcgaccgg cgtggggatc gcgaagatgc gcctgttggt
     2221 cgcgatgcgc agaaacgcgc ccgcgacgag atcgacgact gcgaagggcg tgcgctccgc
     2281 cagggtgtaa tcgagccacg ctctcgcgag ctcgaagtcc gggtggtcgt cgcggtgggc
     2341 cgcaaggacg acattgacgt cgagcagctt caacgcagct tggtcatcgc tgcatcacgg
     2401 tcgagcagct cccgtagcgc gctgttggag cgcaggttca cgccggccgg cggaccattg
     2461 cctccgcgga acaccggcac ctcgaccggc tcgtgcgctg cgggttcggc cagctctcgc
     2521 cgcagggcgc cctcgacgac ctgaccgagc gtctcgccgc gctcgcgtgc ccgccgcttg
     2581 gccgcctcca gcagatggtc gtccacggcg atcgtagtgc gcatgatgcc caaagtagca
     2641 catcaagcac atcaccgagc cgtatcgcgc gtgatccatc tcgagagaaa ggcgcgcggc
     2701 atggaccgag ggggtcagaa acaccgtcgc ccgggtatcg gaatcctatg aaagagggag
     2761 acaaaggttg ccgccctcca caacccccac atagcttcgc cctgcaggac tatctgaaac
     2821 cagaaaaacg caagggggac ttgtgactgg aagcaggagc acacgtacac gcagcacgat
     2881 gctggcgctc gcgacggccg ccgtcgcgct gctcggcgtc ctggggatga cctcctcggc
     2941 gtacgccgtc acgcaccacc cgaaaggcga attcgcgccg ttcgcggact gcccgctgag
     3001 caacccggcg gcggcgcagt gcctggtcgc gaagaccgaa agcggcgaaa tcatcgtcgg
     3061 caaagaagac gtgccgatca cgaacgcgat cacgctgcag ggcgggatcg aattcaccga
     3121 agcaggcgaa aaactgatcg ccgccgaaga cggcaacacg ctgtcgaaaa gcccgcagaa
     3181 agttcccggc gggctgtcgg gcctcgtgaa gtgcaacgaa atcagcaact tcatcgagcg
     3241 gatcgcgtgt gaagtcgtgt tcgaaaacgg cctcaccggc gtgacggcga cgaccgaact
     3301 ggccgcgccg gccagctcgg tcaaaatcga ccagggcgct ctgatcttcc agagcggcac
     3361 ggcgctgtcg ctgccggtca agctgcacct cgaaaacccg ttgctgggga gcagctgcta
     3421 cgtcggctcg aacgcgcacc cgatcaacat cgaactgacg acgggcacga ccagcccgcc
     3481 ggcgccgaac aaaccgatca cgggcagccc cggcgaaatc ggcttcaacg aagccgaaac
     3541 gctgatcacg ctgaaaaaca acgtgctcgt gaacaactcg ttcccggctc ccgtcgccga
     3601 aggctgcggc ggtctgttcg ccttcctgat cgatccgatc atcgacagca ggctcggcct
     3661 gccgtcggcg gccggccaca acacggcgat cctcaggggg acgctgaagg cggcgacggc
     3721 cgaagcggtc aaagcgagcg aatagctctc tgacgacgta gacgccgaag acgcacgtga
     3781 gggctcgccg cgccagcggc gggccccgcg tccgcccgcg tccgcccgcg atcgcccgcg
     3841 atcgcccgcg ggcgggcgcg tccgtgcagt cggagacatt tcgcccggtc gcttgacgcg
     3901 ctttcctcgc ctacgcgata ttcgggcgcg ccatgctcgc ccgcagcccg cacatgcgat
     3961 cgccggcggc cggcttgccg gccgccgtcg tgctcgccgt tgcggcgctc ggcgcgtgcg
     4021 gctgctccgg aaacacggcg acgacctcga cgagcgcctc gagcgcgacc tcgacgtcgc
     4081 gggtctcgac ttcgcgtgtc gcgacgtcga ggcccgccgc cggaggagac agcgcgacgt
     4141 tcatcgcgcg cgccgacgcc gtttgtcggc gcctgaacgg tcagctggcc gcgacggcgc
     4201 cgcatcaggg cgaagcgccc gcagcgctcg cccgcaacgc cctccagcac gccgcgctcg
     4261 agcgcaggac ctcgagcgag ctcgccaagc tcccgccgcc ccgcgcgctg gcgcgtgact
     4321 gggcgcggat cgtcgccgac aggcgcactc tcgcggcaga cctcgtcacc ctcgcgcggg
     4381 cgttgagctc tggccagagc ccgcagacca tcggggtgct cgccctcaaa tcggccacgc
     4441 acaaggcgat gtcggcgatc gccgagcacg acggcttcgc cgactgcgcg aaggttggat
     4501 cggcctagcg gcgccagtct gcgccggccg gatcgctcct gccgggcgcg ctctgactat
     4561 tggaaacctt ggtcggagac aaagacacgt tccgcttgac tcgccgccga acgccgtggc
     4621 agactggccg cgccgttgat cctcggcaat ccggtgatct tgctccggaa gccggggatc
     4681 gttaccgggc agtggccggc tcgactcgtc tggccggcga ctgccgcaat aagccaagca
     4741 gaagagataa ggagcgtttc acaatgaagc gattcaagct gctgggtctg gcgctggttg
     4801 ccgtgttcgc acttgcggcc gccttctccg cgtcggcctt cgcgttaccc gaactgttag
     4861 gtcagggagc gaacgcaacg tacgaaggca aaaacaccgc cgcgaacccg accctcgaaa
     4921 cgaccaaaag cgaaaaaatc gtctgtaaag aagcgacggc gaatggcgtc caggaaacca
     4981 acagcctggc gtccttccac atcagcttca aagggtgcga atcgtccggc ttcaaatgca
     5041 actcgtcggg cgacgaaaca ggagtcatct tagtgttagg cagcgcccac tacgtggaca
     5101 gcacccctgg caacgtcgca attctgttct tattcgaaga actgacgatc aaatgcacgg
     5161 cgctcgtgac cctgaaggtc aagggcgaca tcgtctgccc ggtgttagaa gcgttaacgt
     5221 cggctgcgac gcatgaattc cactgcaccg agtccgccgg caaaccgtcg acgacatcgt
     5281 ggctcaacga cgaaaacgaa acacagaccg ccaaggtcga aacctcgaaa aacggcggcg
     5341 cgttcatcga atcggccgag caggcattag cttcggtcac gttcggcacc gccgtcgcgg
     5401 tgatgaacga gtagcgatca gctcctcttt cagagaagcc tgaagacgat cgcggcgcgc
     5461 gggggatccc gcgcgccgcc tcgtcgtgct cagggcaagc gggatgcgtt atggcccggg
     5521 atctcgcgat gttcgggaaa ccgagtgaga gacacgcgtt gagagaccgg ccggctgtgt
     5581 ctagcggtgg cagtgcagga ctgggcgaca atcccgtggg ggcgtgagag gagagggctt
     5641 aggtgagtgg tgctgtcgcg cggcagggag agcggcgtct cgccgcgcct cggtcgcgcg
     5701 aggattcgtc ttctctcgag gccctgtcca accgtgtaca tagctcggtc gccgcgaggc
     5761 ggcgaccggc ccaaccgcgg ccgggcgcgc cgcgcgccga cgttcgagcc gtgtagaagg
     5821 aaggaaggcc cccaccgatg ttctcccccc tacgcaaacg aattcgtgtg agcccggcga
     5881 gcgcgctggc ggtcgccgcg ctggtgctcg caatgaccgg cggcgcatac gcggccggca
     5941 ggtacgtcat cacctcgacg aagcagatca agccgagcgt tctgaaggcg ctgaagggga
     6001 acgccggtcc cgccggcaag gctggcgcgg cagggcccgc cggttccgcc ggctcgcagg
     6061 gagccgaagg caagcagggt cccgaaggca aacagggtcc cgaaggcaaa gcgggccccg
     6121 aagggaaatc tggtgaaaaa ggagcgaccg gctcaccatg gacggccggc gggacgctcc
     6181 ccaaaggcgc gaccgagacc ggtacgtggt tcgccggcgt caattccgaa aagctgggct
     6241 tcgacgcgat ctcgttctcg atcccgctcg ccggcgcgct cgacgccgaa cacgtccact
     6301 acgtcacgat caaagaagtc gaagcagcga aagcgcctgc cgcgtgcccc ggcagcgccg
     6361 cggcgccggc agcccgatca ggcagcttct gcgtatacga aggcgcgctc tccaacccgc
     6421 agggcggcgt tgaagaagcg atcatcgagg acgccacggc cgcgaccgct ggcgctgcga
     6481 cgaccggagc cgccctggtg gtcgtggcga aagcgaccga atcgacgttc tggggcagct
     6541 gggcggtcac gggttaggcg gcacgcccgc cccgaggcac ggaaatggag ctcggcaagg
     6601 tgcaaccgac gacagcacgg tcgctcatgc gtcgctgtgc cgcggccgga gggctggcgc
     6661 tctgcctgct cgccggcctg ctgggcatcc tctcctcgcc ggcgtcggcg ttcaaaaccc
     6721 gcccgctgct gggcaagttc ggcgcgctga gcaatccgca cgggatcgcg atcgaccagt
     6781 cgaccggcga tctgtacgtc gccgacacgg ccaacaaccg gatcgagaag ttcgacgaaa
     6841 ccggcaaatt cctgctggcc ttcggcgcga acgtaggcgg cgccggcgtc aacacctgca
     6901 cgtcgggctg cgccgccggg acgagcggat cggcccccgg ccagttcacg acggcgacgt
     6961 tcgtcgcggt cgacaacagc gccagcgcct cgaaaggcga cgtctacgtc ggcgacgtcg
     7021 gcgacaacaa ggtgtcgaag ttcacctcct ccggcgaact cgtctcgagc tggggcacgg
     7081 gtggacagct cgacggctcg acgaccggct tcaaaacgtt tgctccgctc gcgggggtcg
     7141 cggtcgactc gagcggcaac ctgttcgtgc tgaagggtca gaccgaaccg ggcaggctgt
     7201 tcaagttcac gcagagcgga agcttcgtca cagaagaatt cggccactcc ttcctgcgcc
     7261 tcaacgcgac cctcggactt gcgctcgacg ccacggggaa catcttcaag gtcaacggcg
     7321 acggctccgt cgacaagctg aaaagcaacc tgcaggaaat cggcaccgtg acgccgcaga
     7381 aaccggcggc gaaaacccgc gcgctggacg tcgacccggc gacgggagac ctgttcctcc
     7441 cgacctcggc cggcacgctc gaccactacg cgttcaacgc ctccgaccag gtgatcgagc
     7501 cgaacggccc gccctgcccg ctcgtcgagg cggtcggttg tacgccgtcg gactcggcga
     7561 gtatcgggtt cgtcggcgcc ggcatcgccg tcaacgccgc gagcgaagac acctacgtct
     7621 cgaaccccac caccggccag gtcttcgagt acggaccgct cgtcacggtc ccggacgtca
     7681 cgaccgaagc cgcgacgaac gtcgtctcga ccgcggcccg cttcaacggg aaggtgaacc
     7741 ccgacgaact cgccgtgacc acgtgcgaat tcgaatacgt cagcgacgcg caactggtcg
     7801 aactgaacgg caaagactac gaagaacgca tcgaaagcgg cagctccccc gcggaaatct
     7861 tcgaagcggt cgggtcgcac gcggcatgcg aacaccccga cgcagccgaa atcggcgccg
     7921 gcgcaagcaa cgtcgccgtg cacgcggacg tgaccggcct gacctccggc gcgcgctacc
     7981 acttccgcct cgtcgccgcc aacgcgaacg gccccaacgc cggctcggtc ctgagcttcg
     8041 tcaccgtcac gccgccgacg atcgaaggcg ccgaagccag ggacctcacg ccgaccacgg
     8101 cgaacctcga agccgagatc aacccgggca gcggcgagac gacctatcac atcgaatatg
     8161 ggacctcgac ctcgtacggc acggtggtcc cagtgcccga cggggacgtc ggctcgggcg
     8221 cgagcgacgt gcccgtcgcg cagcacgtcg ccgggctgat ctccggcgtc gcctaccact
     8281 ggcgcgtcgt cgcgtcgaac tcggccggca cgacgatcgg cgccgatcac gtgttcatct
     8341 tcaaaacgat cgccgcgacg ctccccgacg gtcgcgcgta cgagatggtg acgccgccgc
     8401 agaagaacgg ctcgctgatc ggcgacaccg gcggcctcgg cctccagccg agcatcgccg
     8461 ccggcggcgg acgcctgatc gcgacgtcgc tccagtgctt cgccggcgcg cagtcctgca
     8521 acgcgcagca cggcgtgggc atcggctctc cttactcgtt cgaaaggacg agcaccgctt
     8581 ggatcacgac cccgctcgcg ccgcccgcca cgggcgaaag ccctgcaaac acgccgtgga
     8641 tctacctggc cgacaacggc tccgcgctgt tctcgatgcc gacgccgccg ttcggcgtcg
     8701 acagcctgtt caagcgcgaa gccggcggcg gcttcgtcgc gatcggcccg atcagcccat
     8761 ccgaactcgg tccgcagacg ccggtcgccg ggaagccggg cgccttcgtg caggctcaga
     8821 ccgccgacct gtcccacata gcgtggagca gcggcagcgg gccgttgcac tggccgttcg
     8881 acgcgaccga agtcaaacac tcctcgatct acgagtacac agggaccggc aacgcgcggc
     8941 cgttcctagt cggcgtcacc ggcggcgc
//
LOCUS       DASUCJ010000715         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_84082, whole genome shotgun sequence.
ACCESSION   DASUCJ010000715 DASUCJ010000000
VERSION     DASUCJ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84082"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..550
                     /locus_tag="VFU90_08575"
     CDS             83..550
                     /locus_tag="VFU90_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479871.1"
                     /translation="MQTGTLAYTLLVLAAFVAVAFLVVPGLILELVGRTHDVVLIRKT
                     YALASFHGRVGGPFSILILLFGLWAAVANGIPLGTTWLVISYVLYAILAGTGFGYHMR
                     RELRIAGLSQTSPDSAPSPELAAAIDDPLATPMLWVSGILWIALIVVMILRPG"
     gene            complement(569..1363)
                     /locus_tag="VFU90_08580"
     CDS             complement(569..1363)
                     /locus_tag="VFU90_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5479872.1"
                     /translation="MSQDGGAGGANGVPLRVEKLSAYYGARRAVADVSMGFAANAVTA
                     LMGPSGCGKTTILRCLNRIHEIAPGAYAEGSVLLHGEDVYDDMSVIELRRRVGMVFQR
                     PNPFPTMSILANVTSGLGRGVPAGEKMATAEKALRAAALWEEVKDKLHKPATALSGGQ
                     QQRLCIARALAVEPEVLLMDEPTSSLDPISTLSIEELMTALAADYAIVVVTHNMQQAA
                     RVSDYSAFMLADEPGVGRLVEMDETTKIFTKPSDRRTEDYITGRFG"
     gene            complement(1360..2232)
                     /gene="pstA"
                     /locus_tag="VFU90_08585"
     CDS             complement(1360..2232)
                     /gene="pstA"
                     /locus_tag="VFU90_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5479873.1"
                     /translation="MSAASAPLQPVRRALGLRHAVSWVGTAVTAVCASIGAVSLLIIL
                     GYVAFAGFRAVNFAFLTQLPVPVGEPGGGVANGIVGTLITVGLATLIATPIGLLSGLF
                     LALFGRGWFPEAVRFLSDVLSGIPSIAIGLFAYTLLVAPFRHFSAVSASFAFALLMLP
                     LILRTSEEAVRSVPRAIREGALALGLSEFSATMRVVVPAARPAIITGLLLAIARVTGE
                     TAPLLFTAFGSQFWEVNPVQPMAQLPLQVFTYAISPYQTWHQQAWAGALLLILAVLVL
                     NVLARLVLTPRSGR"
BASE COUNT          407 a          806 c          712 g          379 t
ORIGIN      
        1 ctttcggtcg agaggattcg acggccgtaa ggaattccac gcgcgtattg ggaagaaggc
       61 gagcgcttgg aggcactcat tcatgcagac gggtaccctt gcgtatactc ttctcgtgct
      121 cgcggcgttc gtcgcggtgg cctttctggt ggttcccggg cttatcttgg agctcgtcgg
      181 gcgcactcac gacgtcgtgc tgattcgcaa gacctacgcg ctggctagct ttcatggccg
      241 cgtcggcgga ccgttttcga ttctaatttt actgttcgga ctctgggccg ccgtcgccaa
      301 cggcattccg ctcggcacga cgtggctggt gatctcctac gtgctctacg cgattctggc
      361 cggcaccggc ttcggctatc acatgcggcg cgagcttcgt atcgccggcc tgtcgcaaac
      421 ctcgcccgat agcgcaccct ctcccgagct cgccgccgcc atcgacgacc cgctcgccac
      481 gccgatgttg tgggtgagcg gtatcttgtg gatcgcgctg atcgtggtga tgatcctgcg
      541 gccgggctga acctacgcgc gtgcgacgct atccgaaccg gcccgtgatg tagtcttcgg
      601 tgcgccggtc ggagggtttg gtaaatatct tggtggtttc gtccatctcg acgaggcgcc
      661 cgacgcccgg ctcgtcggcc agcatgaacg ccgaataatc ggaaacgcgc gcggcctgct
      721 gcatgttgtg ggtgaccacg acgatcgcgt aatcggcggc cagcgccgtc atcagctctt
      781 cgatgctgag cgtcgaaatc ggatcgagcg aagacgtcgg ctcgtccatc agcagcacct
      841 cgggttcgac ggccagcgcg cgcgcgatgc acagccgttg ctgctgcccg cccgagagtg
      901 cggtcgcggg cttatgcagc ttgtctttca cttcttccca cagcgccgcc gcgcgcaacg
      961 ccttttcggc cgtcgccatt ttctcaccgg ccggaacgcc gcgccccagt cccgacgtca
     1021 cgttcgccaa gatcgacatg gtcggaaacg gattcggacg ttgaaacacc atgccgacgc
     1081 gccggcgcag ctcgatcacg ctcatatcgt cgtacacgtc ctcgccgtgc agcaggacgg
     1141 agccttccgc gtaggcgccg ggcgcgatct cgtgaatccg gttcaaacag cgcaggatcg
     1201 tcgtttttcc gcacccggac ggccccatca gcgccgtgac ggcgttggcc gcgaaaccca
     1261 tggacacgtc cgccaccgcc cgccgcgcac cgtagtaggc cgatagtttt tcgacccgca
     1321 gcggcacgcc gttcgcgccg ccggcaccgc cgtcttggct catcgccccg accgcggcgt
     1381 cagcaccagc cgcgccagca cgttgagcac cagtacggcc agaatcagca gcagcgcgcc
     1441 cgcccacgcc tgctggtgcc acgtctggta cggcgaaatc gcgtaggtga acacctgcag
     1501 cggcagctgc gccatcggct gcaccgggtt cacctcccag aactggctcc cgaacgcggt
     1561 gaacagcagc ggcgcggttt cgccggtcac gcgcgcgatc gccagcagca acccggtgat
     1621 gatggccggc cgtgccgccg ggacgacgac gcgcatcgtc gcggaaaact ccgaaagccc
     1681 cagcgccaac gcgccctcgc ggatcgcacg cggaacgctg cgtaccgctt cctccgacgt
     1741 ccgtaagatg agcggcaaca tcagcagcgc gaacgcgaac gacgccgaca ccgccgaaaa
     1801 gtggcgaaac ggcgcgacca gcagcgtata ggcgaacagc ccgatcgcta tgcttggaat
     1861 tccggagagc acgtcggaca gaaagcgcac cgcctccggg aaccagccgc ggccgaacag
     1921 cgcgagaaac aaacccgaca gcagaccgat cggcgtcgcg atcagcgtgg ccagcccgac
     1981 cgtgattaac gtgccgacga tgccgttggc gactccgccg cccggctcgc ccaccggcac
     2041 cgggagctgc gtgagaaacg cgaagttcac cgcgcggaaa cctgcaaacg cgacgtaacc
     2101 gagaatgatc agcagcgaga ccgcgccgat cgaggcgcag accgcggtga ccgcggtccc
     2161 cacccagctg accgcatggc ggagtcccag cgcgcgccgc accggctgca gcggcgcgct
     2221 cgccgcgctc actttttctc cctcaggacg gcccagagca gcagccgcgc catcgcgttg
     2281 acgataaagc tcaccacgaa aagc
//
LOCUS       DASUCJ010000716         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_466223, whole genome shotgun sequence.
ACCESSION   DASUCJ010000716 DASUCJ010000000
VERSION     DASUCJ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466223"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFU90_08590"
     CDS             complement(<1..434)
                     /locus_tag="VFU90_08590"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479874.1"
                     /translation="MKRSHIVVRICVVAFSMVLPSIALAAPGSLDPAFGHGGFVVYPG
                     DPYAVTDIKVQSNGRILISGDMAGFAGGIGGFSVLRLLPGGKPDASFGVGGLAVAKFG
                     SKINTAESLAIQSDGKIVAAGLTAVDVPRGSDAMAIARFRPGG"
     gene            468..>1044
                     /locus_tag="VFU90_08595"
     CDS             468..>1044
                     /locus_tag="VFU90_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479875.1"
                     /translation="MKRALKKVAVLSRTTWTTPPGYLSYNRHIPSNGILIAFAHRPYD
                     DGQKENASMNALTRKDFVAGVAVAGAAIASGCSTPSQNASSGSTSVEGLGNDARSIIA
                     ARKLSATDVTAALRTFVPSARFDDYYMFASGGHSGQVVVIGIPSMRILKIIPVFTPDA
                     YGGWGYSTESKGVLAAGGVGDRTILHGDTHHP"
BASE COUNT          209 a          356 c          320 g          159 t
ORIGIN      
        1 ccgccgggcc tgaagcgggc gatcgccatc gcgtccgaac ctcgaggtac gtcgactgca
       61 gtgagaccgg ccgcgacgat cttcccatcc gattgtatcg caagcgattc cgcggtgttg
      121 atcttcgacc cgaacttggc cacagcgagg ccgccgacgc cgaacgaggc gtcgggcttg
      181 ccgcccggca gcagtctgag gacggaaaac ccgccgatcc cgccggcgaa accggccatg
      241 tcgccgctga tgaggatccg gccgttcgac tggaccttga tgtcggtcac cgcgtacggg
      301 tcccccggat agacgacgaa accgccgtgc ccgaacgccg gatcgagcga gcccggcgca
      361 gcgagcgcga tcgacggaag aaccatggaa aaggcgacga cgcagatgcg tacgacgata
      421 tgcgagcgct tcatgcgtgc atcgtacgga cacggactga aacgaccgtg aaacgagcgc
      481 tgaaaaaggt ggccgttctg tctagaacga cctggacgac gccgccgggc tacctttcct
      541 acaaccgcca cataccttcc aacggcattc tcatcgcctt cgcacaccgg ccatacgatg
      601 atggccagaa ggagaatgcc tctatgaacg cattgacccg caaagacttc gtcgccggcg
      661 tggccgtcgc gggggctgcc atcgccagcg gctgttcgac gccgagccaa aacgcgagta
      721 gcggatccac ctccgtagaa gggctcggca acgacgcccg cagcatcatc gccgcccgca
      781 agttgagcgc gaccgatgtc acggcagcgc tccgcacgtt cgtcccgtcg gcccgcttcg
      841 acgactacta tatgttcgca tcgggcgggc acagcggcca ggtcgtcgtc atcggcatcc
      901 cgtcgatgcg catcttgaag atcatccccg tcttcacgcc cgatgcgtac ggcggatggg
      961 gttactcgac cgaatcgaag ggcgtgctcg cagccggcgg cgtcggcgac cgcacgatcc
     1021 tccacggcga cacacatcac ccgg
//
LOCUS       DASUCJ010000717         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1108145, whole genome shotgun sequence.
ACCESSION   DASUCJ010000717 DASUCJ010000000
VERSION     DASUCJ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108145"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /gene="ygiD"
                     /locus_tag="VFU90_08600"
     CDS             <1..725
                     /gene="ygiD"
                     /locus_tag="VFU90_08600"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEU5479876.1"
                     /translation="NALELNRYSEAWRRLGTALPRPSAILAVSAHWYSDGVALTAMER
                     PRTIHDFYGFPRELYAMRYPAPGAPELAARVAELLAPVEVRLDHGWGLDHGTWSVLAH
                     LFPEADVPVVQLSLDARRPAAFHWDLGAALAPLRDEGVLVLGSGNAVHNLHLVDFGMP
                     GGFDWAVRFDEHVRAAVDGSDRDGLVEYLRHPDGRLAVPTPEHYLPLLYVAALRRGDE
                     PVTTIVEGLEAGSLSMRAIQVG"
     gene            984..1409
                     /gene="msrB"
                     /locus_tag="VFU90_08605"
     CDS             984..1409
                     /gene="msrB"
                     /locus_tag="VFU90_08605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5479877.1"
                     /translation="MTNDSKASGHAPEVRKSEAEWRDALGPERYHVLREAGTEAPFSG
                     SLLNVRDDGTYVCGGCGAELFGSDAKFDSHCGWPSFTHPEEQQNVRLLDDDSFGMHRT
                     EVRCRRCDSHLGHVFDDGPGPRGTRYCINSRSLDFRKRA"
     gene            complement(1406..1903)
                     /locus_tag="VFU90_08610"
     CDS             complement(1406..1903)
                     /locus_tag="VFU90_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479878.1"
                     /translation="MNARLLALALAAGAALAACSSSKTTTVQTRNGAATITTSQGGEN
                     VTVQTSEGTTSLGSHVDPSKLGVSIYPGAEADAQGIITTKEARGTSTVAAFKTADSFD
                     KVYAYYKDRLPAGSERMKVTSGNGSVASFQIGSPKTPDEVTVQVSSDKPNETDLLITR
                     VVRAR"
     gene            1965..2894
                     /locus_tag="VFU90_08615"
     CDS             1965..2894
                     /locus_tag="VFU90_08615"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5479879.1"
                     /translation="MTVKAQFPDEQTHGGEFVRQPDAFREWVRADGSSAYPAVAGRYH
                     LYVSLACPWAHRTIVVRKLKRLEDAIGMTVVDPVRDERGWRFVEPDPLNGWAFLSEAY
                     FASDPAYRARVTVPVLWDKQHRRIVSNSDGDIMRMLESEFDAFGDASLDLYPRDKRHE
                     IDELNELIYETVNDGVYRAGFATAQRPYERAAYKVFETLDKLDERLATQRYLFGPRPL
                     ESDWRLFVTLVRFDAVYYGHFKCNLRRIADYPNLSGYLRDLYQIDGVAETVDFDHIKR
                     HYYYTHDDINPTRIVPIGPLQDLGAPHGRERLT"
     gene            2967..3599
                     /locus_tag="VFU90_08620"
     CDS             2967..3599
                     /locus_tag="VFU90_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479880.1"
                     /translation="MKNLWLALALALLAVPASAMAQDAPSAAAVPAPPPHPQLTPAQR
                     QAMFKTMSDFHDKEMQLHKQLRSQMLGALSPEHRTAVANVIGQLAISASPDPGAAAKQ
                     IDALLTPAEKQGITTAHTQFSDQSRTLMQQMMSQMKSEMPSPPPGAPPHGTMMGQNWQ
                     KHNEVVDPGTILLTTLNGGGRMGMMMAHGFGMPPGPGMPPGPPGGGPPPP"
BASE COUNT          608 a         1171 c         1205 g          644 t
ORIGIN      
        1 tgaacgcgct cgagctgaac cggtactccg aggcgtggcg gcggctcggc acggcgctgc
       61 cgcgcccgtc ggcgattctg gccgtttcgg cgcattggta ttccgacggc gtcgcgctca
      121 ccgcgatgga gcggccgcgc acgattcacg acttctacgg atttccacgc gaactgtacg
      181 cgatgcgcta tccggcgccg ggtgcgcccg agctggcggc ccgcgtcgcg gaactgctcg
      241 cgccggtgga ggtgcggctc gaccacgggt gggggctgga tcacggaacg tggtcggtgc
      301 tggcgcatct cttcccggaa gcggacgtcc cggtggtgca gctgtcgctc gacgcgcgcc
      361 gcccggccgc ctttcactgg gatctcggcg ccgcgctcgc gccgctgcgt gacgaaggcg
      421 tcctggtgct cgggagcggc aacgccgtac acaacctgca cctggtcgat ttcggaatgc
      481 cgggcggctt cgactgggcg gtgcgcttcg acgagcacgt gcgcgccgcg gtcgacggtt
      541 ccgatcgcga cgggctggtc gagtatctgc gccatcccga cggccggctg gcggtgccga
      601 cgccggaaca ctatctcccc ctgctgtacg tcgcggcgtt gcgccggggc gacgagccgg
      661 tgacgacgat cgtcgaggga ctggaagcgg gctcgctctc gatgcgcgcc atacaagtcg
      721 ggtagtccgg cgacaccgag ggtactccaa caaaaatccg tcgtacttcg cgagagtgac
      781 cggaattttt gttggagcac cctcggtatc gccttagagg tttgaccgtt cgtccttcga
      841 ctgcgcaccc ttcgggcgct ccgctcagga tgacacggtt ggggcgctcc gctcagccct
      901 tcgactgcgc gtgcttcgca cgctccgctc aggatgacac ggggcgcatc cggggcggcg
      961 ccgccggcgt tctacgtagt gctatgacca acgattcgaa agcctccgga catgcgcccg
     1021 aggtccggaa gagcgaagca gaatggcgcg acgcgctcgg gccggagcgc taccacgtat
     1081 tacgcgaagc cgggacggag gcgccgttca gcggcagctt gctgaacgtt cgcgacgacg
     1141 gtacctacgt ctgcggaggc tgcggggcag agctgttcgg gtcggacgcg aagttcgact
     1201 cgcactgcgg ctggccgagc ttcacgcatc cggaggagca gcaaaacgtg cggttgctcg
     1261 acgacgacag cttcggaatg catcggaccg aagtgcgctg ccggcgctgc gattcgcatc
     1321 tcgggcacgt cttcgacgac gggccggggc cgcgcggaac gcgctactgc atcaactcgc
     1381 gctcgctcga ttttcgcaaa cgcgcttagc gcgcgcgaac gacgcgggtg atcagcaggt
     1441 cggtttcgtt tggcttgtcc gacgatacct gtacggtgac ttcgtcgggc gtcttggggc
     1501 tgccgatctg gaacgacgcg acggatccgt tgccgctggt gaccttcatc cgttcggagc
     1561 cggccggtaa gcggtctttg taatacgcgt acactttgtc gaacgaatcc gccgtcttga
     1621 aggcggcaac ggtgctggtt ccgcgggctt ctttggtcgt gatgattccc tgtgcgtcgg
     1681 cttcggcgcc cggatagatc gacacgccaa gcttcgacgg atcgacgtgc gaaccgagcg
     1741 aggtggtgcc ctcgctggtt tggacggtga cgttttcgcc gccctgactc gtcgtaatgg
     1801 tggccgcgcc gttgcgcgtc tggacggtcg tcgttttgga cgacgagcac gccgccagcg
     1861 cggcgccggc cgcgagcgcg agggcgagga gccgagcgtt cataaccgcc catttccgtt
     1921 tgcggacgga aaacccattg ccgcccgcgg gggttgtaga cggcatgacg gtcaaggcgc
     1981 aatttcccga cgaacaaacg cacggcgggg agttcgtgcg tcagccggat gcgtttcgcg
     2041 aatgggtgcg tgcggacggc agctccgcct acccggcggt agcaggacgg tatcacctct
     2101 acgtctcgct tgcgtgcccg tgggcgcatc gcacgatcgt cgtgcgcaag ctcaaacgcc
     2161 tcgaggacgc gattgggatg acggtcgtcg acccggtgcg cgacgagcgc ggctggcggt
     2221 tcgtcgagcc ggatccgctc aacggctggg cgtttttgag cgaagcgtac tttgcgtcgg
     2281 accccgcgta tcgcgcgcgg gtcaccgtgc ccgtgctgtg ggacaagcag catcggcgca
     2341 tcgtcagcaa ttccgacggc gacatcatgc gcatgctcga gtcggagttc gacgcgttcg
     2401 gcgacgcgtc gctggacctc tatccgcgcg acaaacggca cgaaatcgac gagctcaacg
     2461 agctgattta cgaaaccgtc aacgacggcg tgtatcgcgc cggcttcgcc accgcccaac
     2521 gcccctacga gcgcgccgct tataaagtgt tcgaaacgct cgacaagtta gacgagcggc
     2581 tggcgacgca gcggtatttg ttcgggccgc gaccgctcga gagcgattgg cggctgttcg
     2641 taacgctggt gcgcttcgat gcggtatatt acggccattt caagtgcaac ctgcggcgga
     2701 tcgccgacta cccgaacctg tcggggtact tgcgcgacct ctatcaaatc gacggcgtcg
     2761 ccgagacggt cgacttcgat cacatcaagc gccactacta ctacacccac gacgacatca
     2821 acccgacgcg aatcgtcccg atcggcccgc tccaggacct cggcgccccg cacggccgcg
     2881 aacgcctaac gtaattctca tgcagcggcg gggcgagcga gcgtttctca gccgtggtta
     2941 ggatagactg gtatatcatg ctttctatga agaacctttg gctcgcgctt gcgctagcgc
     3001 ttcttgccgt acccgcttcc gcgatggctc aagatgcacc gtcggcggcc gcggtgccgg
     3061 ccccgccccc gcatccccaa ctgacgccgg cgcaacgcca ggcgatgttc aaaacgatgt
     3121 ccgacttcca cgacaaggaa atgcagcttc acaaacagct gcgctcgcag atgctcggtg
     3181 cgctttcacc cgaacaccgc accgcggtag ccaacgtcat cgggcagttg gcgatctcgg
     3241 cgagccccga tccgggcgct gccgccaagc agatcgacgc gctgttgacg ccggccgaaa
     3301 agcagggcat cacgaccgcg cacacccagt tttcggatca gagccgcact ttgatgcagc
     3361 aaatgatgtc gcaaatgaag agcgagatgc cgtcgccgcc gccgggcgcc ccgccgcacg
     3421 gaacgatgat gggtcagaac tggcagaagc acaacgaggt cgtcgatccg gggaccattc
     3481 tgctgaccac gctcaacggc ggcggccgaa tgggcatgat gatggcgcac ggtttcggaa
     3541 tgccgcccgg ccccggaatg ccgccgggtc cgcctggtgg tggaccgccg ccaccctaag
     3601 ctttacgcct gcagtgacgt cgagggca
//
LOCUS       DASUCJ010000718         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_863588, whole genome shotgun sequence.
ACCESSION   DASUCJ010000718 DASUCJ010000000
VERSION     DASUCJ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863588"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="VFU90_08625"
     CDS             <1..>1077
                     /locus_tag="VFU90_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEU5479881.1"
                     /translation="IATLAELFDPDPLTAEGRLPLDSAQIGALDALLHEPGLSWLGAS
                     RARALIERLDAAPAGAVAVPAAFHGTLRAYQHAGVGWLQALGDYGFGALLADDMGLGK
                     TVQLLAHVSIERARGRLNAPVLIVAPTSVVPNWRAEIARFTPELRQLSLTGADRGGRF
                     SEIDGADIVLTTYALLQRDAERLLAREWSLAVLDEAQAVKNPRTKGAQVARRIRARQR
                     IALTGTPIENNLDELWSLFAFAVPALLGDRAAFGRRFRTPIEKHGDEKRRDSLAARVR
                     PFLLRRTKEAVEADLPPKSEIVQRVELAGAQRDLYETIRLALHDRVRAELRRRGAERS
                     RIVVLDALLKLRQVCCDPRLVKLP"
BASE COUNT          163 a          354 c          372 g          188 t
ORIGIN      
        1 tgattgccac gctcgcggag ctcttcgacc ccgatccgtt gacggccgaa ggccggttgc
       61 cgctcgattc tgcccagatt ggagcgctcg acgcgctcct gcacgagccc ggactttcct
      121 ggctgggcgc atcgcgagca cgcgccttga tagagcgtct cgatgccgcg ccggccggcg
      181 ccgtcgcggt tcccgcggcg tttcacggga cgctgcgggc ctatcagcac gccggggtgg
      241 gatggctaca ggccctcggc gattacggtt tcggcgcgtt gctggccgac gatatgggtc
      301 tgggaaaaac cgttcagctg ttggcgcacg tttcgatcga acgcgcgcgc gggcggctga
      361 acgctccggt gttaatcgtt gcaccgacga gcgtggttcc taattggcgc gccgagatcg
      421 ctcgctttac gccggagttg cggcagctgt cgctgaccgg cgccgaccgc ggcggccggt
      481 tctccgagat cgatggcgcg gacatcgtct tgaccacgta cgccctgctc cagcgcgatg
      541 cggagcgcct gctcgcacgc gagtggtcgc tcgcggtgct cgacgaagcg caggcggtaa
      601 agaatccgcg aaccaaaggc gctcaggtcg cacggcggat acgcgcccgc caacggatcg
      661 cactcaccgg aacgccgatc gagaataatc tcgacgagtt gtggtcgttg ttcgcgtttg
      721 ccgttccggc actgttgggt gaccgggcgg cgttcgggcg ccgcttccgt acgccgatcg
      781 aaaagcacgg cgatgaaaaa cggcgcgact cgctggccgc ccgcgtgcgg ccgttcttgc
      841 tgcggcgcac gaaggaagcg gtcgaagccg atttgccgcc caagtccgag atcgttcaac
      901 gcgtcgagct ggccggggcg cagcgcgacc tgtacgagac gattcgcttg gcgttgcacg
      961 accgcgtgcg cgccgaactc cgccgccggg gcgcggaacg cagccggatc gtggtgctcg
     1021 acgcgctcct gaagctccgc caagtgtgct gcgatccgcg cctggtgaaa ctacccg
//
LOCUS       DASUCJ010000719         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1306545, whole genome shotgun sequence.
ACCESSION   DASUCJ010000719 DASUCJ010000000
VERSION     DASUCJ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306545"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFU90_08630"
     CDS             <1..289
                     /locus_tag="VFU90_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5479882.1"
                     /translation="RVVVGVNKFTTDGAEPYQPLRVDPALEAEQVERVRAVRSARDAR
                     AASASLDAVRAAAAGDDNVLPPIRSALAARATVGVVCAALRDVWGSYEPSG"
     gene            410..679
                     /locus_tag="VFU90_08635"
     CDS             410..679
                     /locus_tag="VFU90_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479883.1"
                     /translation="MTLVIVVAAIGALVLLVSILALSRSRFADESERFRYVSDLTSKW
                     SRQQQSQSQPAMPATAVPAGPSEAGEAAAENSIDLRDAERGARRS"
BASE COUNT          178 a          340 c          362 g          145 t
ORIGIN      
        1 ccgggtggtc gtgggtgtga acaaattcac aaccgacggc gccgagccct accagccgtt
       61 gcgggtcgac ccggcgctgg aggccgagca agtcgagcgg gttcgcgccg tccggtctgc
      121 tcgcgacgcg cgcgccgcga gcgcgtcgct cgacgccgtt cgggcggccg cggccggaga
      181 cgacaacgtg ttgccaccga tcagatcggc gctcgcggcg cgcgcgaccg tgggcgtggt
      241 gtgcgcggcg ttgcgcgatg tgtgggggag ctatgagccc agcggatagc gcggaccgac
      301 cgaaaggagg gctatcgaaa cctgcttcgt agcaggacgc cggagaagcg cgccgcgccg
      361 agatcccttg cgctggcccg ctttgctcga acgaaaggaa ccactcgcca tgaccctcgt
      421 catcgtcgtc gcggcgatcg gagcgctcgt gctcctcgtg tcgatcctgg ccctcagccg
      481 gtctcggttc gccgacgaga gcgagcgatt tcgatacgtg tcggacctca cgtcgaagtg
      541 gagtcgccag caacagtcgc agtcgcaacc agccatgccg gcgacggcgg tgccggccgg
      601 cccgtcggaa gcgggcgagg cggctgcgga gaactcgatc gacctgcgcg atgccgagcg
      661 cggcgcgcgg cgttcgtgaa cggctagatc acggcgccat aacgctttga cgcgaccttg
      721 gcggacggct cagtcaagcg cataatctcc gggatgacgg cagcggcgcc gtcccgggaa
      781 catcgggcgg ccacgcgacg gcgtccggga tcgaggcggc acccgcgcca gagatccgct
      841 agtcccaaca gacccgcttg tcccaaacag accagggatt gattcaccag cggaacgaca
      901 ccggaggcgt cgtgaagaag accgtgccgg gcatcgcagc cattggcgtg actttcgccg
      961 tggcactggc gatgacggcg tgcagcagca agccgaagag ctcagccaac tcctcgtcca
     1021 gcacc
//
LOCUS       DASUCJ010000720         1411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_680080, whole genome shotgun sequence.
ACCESSION   DASUCJ010000720 DASUCJ010000000
VERSION     DASUCJ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680080"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1411
                     /locus_tag="VFU90_08640"
     CDS             <1..>1411
                     /locus_tag="VFU90_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEU5479884.1"
                     /translation="LSVVRPGTITDVYSVNPAVTKAAAPIGGGGGLNNVYSAIAAMPG
                     AYVPNGQMGVNQTVYIRGGYYDQIGYEYDGVPVNRSFDNYPAHSASTLGQQELQIYAG
                     GGGASANATGLAGFINQVVKTGTFPGYANVSGQIGTPTFYHDLSVEAGGATPDRLFSY
                     YVGTSGYNQDFRYLDQSNGADLMNEFPYGAGPSNLTTELPFYPAVYPTCTANATYSNP
                     ATKYSYNDPGCFSAFFPAYSEVSYIAGREVVANLHFGIPHRSDGGRDDVQLLYTSSAQ
                     FRQYYSGVNDGGLQLANGLVDQGWIGRPPHWPDYYTFPSGTQFLAPANVQPIAYLFPG
                     SPTDRCANVSGVPGACPSGTYALLPNDYRDARWDSANIFKLQYQKNFGSSAYLRLFGY
                     TFYSNTNRSGASRRGIGSGYGVENYDYEVDGHTRGLQLDFGDQINSANQFSANINYIT
                     STTLRMNNYNYDNTYYQQV"
BASE COUNT          276 a          525 c          373 g          237 t
ORIGIN      
        1 cgctcagcgt cgtgcgtccc ggcaccatca ccgacgtcta ctccgtcaat cccgcggtta
       61 ccaaagcggc cgcgccgatc ggcggtgggg gcgggctcaa caacgtctac tcggcgatcg
      121 ccgcgatgcc gggcgcgtac gtccccaacg ggcagatggg cgtcaaccag acggtctaca
      181 tccgcggcgg ctactacgat caaatcggct acgagtacga cggcgtcccg gtcaaccgct
      241 cgttcgataa ctatcccgcg cactcggcct cgacgctcgg acagcaagag ctgcaaatct
      301 acgccggcgg cggcggtgcg agcgccaacg ccaccggtct cgccggcttt atcaaccaag
      361 tcgtaaagac cggaaccttt cccggctacg ccaacgtgtc cggacaaatc ggcacgccga
      421 ccttctatca cgacctctcg gtcgaagcgg gcggcgcgac gcccgatcgc ctcttctcct
      481 actacgtcgg caccagcggt tacaatcaag atttccgtta tctcgatcag tccaacggcg
      541 ccgatttgat gaacgaattt ccgtacggcg ccggccccag caacctcacc accgagttgc
      601 cgttctatcc cgccgtctat ccgacgtgca ccgccaacgc cacctactcc aaccccgcga
      661 ctaaatattc gtacaacgat cccggctgtt tctccgcgtt cttccccgcc tatagcgaag
      721 tctcgtacat cgccggccgc gaggtcgtcg ccaacctgca cttcggcatc ccgcatcgca
      781 gcgacggcgg acgcgacgac gtgcagctgc tctacacgtc ctcggcgcag ttccgccagt
      841 actacagcgg cgtcaacgac ggcggcttac agctcgccaa cggcctcgtc gatcagggct
      901 ggatcgggcg tccgccgcac tggcccgatt actacacctt cccgagcggc acgcagttct
      961 tggcgccggc gaacgtccag ccgatcgcgt atcttttccc cgggtcgcct accgatcgct
     1021 gcgcgaacgt cagcggcgtt cccggcgcct gcccgtcggg cacctacgcg ctgctgccca
     1081 acgactaccg cgacgcccgc tgggacagcg ccaacatctt caagctgcag tatcagaaga
     1141 acttcggcag cagcgcctac ctgcggctct tcggttacac gttctactcc aacaccaacc
     1201 gcagcggcgc gagccgccgc gggatcggca gcggctacgg cgtcgagaac tacgactacg
     1261 aagtcgacgg ccacacccgc ggcctgcagc tggatttcgg cgaccagatc aatagcgcca
     1321 accagttctc cgccaacatc aactacatca cctcgacgac gctgcgcatg aacaactaca
     1381 actacgacaa cacctactac cagcaggtca g
//
LOCUS       DASUCJ010000721         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1199792, whole genome shotgun sequence.
ACCESSION   DASUCJ010000721 DASUCJ010000000
VERSION     DASUCJ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199792"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..574
                     /locus_tag="VFU90_08645"
     CDS             185..574
                     /locus_tag="VFU90_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479885.1"
                     /translation="MSRSVDGGKRREWSERLRRFKRCDLTVAAFCETEGVSTPSFYQW
                     RRRLDSLAMEAAPAATRITRQAFVPLRIATPAPAGAPVEIHLPNGARVCLPSNDPQGI
                     AAAIAAAAQAPAVERLNAARGREEQAC"
     gene            568..921
                     /gene="tnpB"
                     /locus_tag="VFU90_08650"
     CDS             568..921
                     /gene="tnpB"
                     /locus_tag="VFU90_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU5479886.1"
                     /translation="MLNLPTHASIFLCVQPVDLRKSFDGLSGLVESVFERNVLDGHLF
                     LFVNKRRDRIKALWWDRDGLALWYKRLERGSFELPSSADGQAQRTLDATELAMLLGGV
                     SLSGAKRRKRFSLAG"
     gene            1057..2826
                     /locus_tag="VFU90_08655"
     CDS             1057..2826
                     /locus_tag="VFU90_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEU5479887.1"
                     /translation="MDEVASQLPPELSPLPAEVPACHALLVEQARSLIELQASREKLS
                     QEIEELKLTVAKLLNQLYGRRSERSIIDPNQLLLEFGWDPAAPDALAAAAAEAETIVQ
                     EFTVRREIQKKSKPRDERLPAHLERYEVIADASDEQKLCPDHGERQVIGYDVTETLEF
                     ERPKLKVRVTKYAKYACVGKPACGIGQAARPNGLVEGNRYGTSVAAEIIAGKFFLHLP
                     LYRQQDQFAASGWTPSRSTLLNIATAATTVLEPLADDYRRRLLQSGGIGCDDTTLTLV
                     TPATIPKLDLNDPRSRRIHEVLSKAHAKGRPSITARMWGYRSFEAPINVFDFTVSRHR
                     DGPDEFLKDYVGSLMGDCWSGFQKIELRSDARIVRAACWSHARRKVFEGRSSHPQQAS
                     VLLALAGALFDIEDRGKLLSAEGRRELRERESRPALDRIRAFVDHPATACVLPKSAFG
                     EALSYLRNHWDALQTYVSDGRMPIDNNDDEQLMRQVAVGRKNWLFVGSLEAGARAATL
                     MTIVSTAARNDLDVYVYLKDVLDQLLVGSTDYRSLHADVWKQSHPEAVRSYRADERRD
                     AADRRHHRRAQRRLAQRSADQPS"
BASE COUNT          551 a          942 c          994 g          501 t
ORIGIN      
        1 gccacccaag agaagaaaaa gacgccgaat ccgaacgtgc ttctagaact tggctatgcg
       61 atcgggcgag ttggttggca acgaataatc ctagtgatga agtaaccgta ctccaccgcc
      121 atgattgacg ctcccggtaa cttgcagagt ttgcgggcac ggactttttg caagggagcg
      181 atcgatgtca cgaagcgtgg atggcgggaa gcggcgggag tggagcgaac ggctgcgacg
      241 gttcaaacgg tgcgacttga cggtcgcggc gttttgcgag acagagggcg tgtcgacgcc
      301 ctccttctat cagtggcgaa ggaggctcga ctcgctcgcg atggaggccg cgcccgcagc
      361 gacgcgcatc acgcggcagg cgttcgttcc gttgcgaatt gcgacgccgg cgcctgccgg
      421 cgcgccagtc gaaatccatc tgcctaacgg cgcgcgagtc tgtctgccga gcaatgaccc
      481 gcagggcatc gccgcggcga tcgcggcggc ggcgcaggcg ccggcggtcg agcggctcaa
      541 cgcggcgcgc ggcagggagg agcaagcatg ctgaacctgc cgactcatgc cagcatcttt
      601 ctttgcgtcc aaccggtcga cctgcgcaag agtttcgacg gactcagcgg cctggtcgag
      661 tcggtgttcg agcgcaacgt cctcgacggc catttgtttc tgttcgtcaa caagcgtcgc
      721 gaccgcatca aggcgctgtg gtgggaccgc gacggcttgg cgttatggta caagcgattg
      781 gaacgcggct cgttcgagtt gccgtcgtct gcggacgggc aagcgcaacg cacgcttgat
      841 gcgaccgaat tggcgatgct gctcggcggc gtctcactga gcggcgccaa gcgacgcaaa
      901 cgattctcgt tggccggttg atcgcgcgag cgagcgatcg cgaccgttcg agcgcgcgac
      961 gtgggacgcg caagccggaa gaactttttt tgttcttccg gctttttttt gcgccgcgtg
     1021 aaaccttggg ccgcatcgcg acactaatcg ggcgtcatgg atgaagtcgc gtctcaactc
     1081 ccgcccgagc tttcgccgct gcccgcagaa gtgccggctt gtcatgcgct gctcgtcgag
     1141 caagcgcggt cgctcatcga actgcaagct tcgcgcgaga agctctcgca agaaatcgaa
     1201 gagttgaagc tgaccgtcgc gaagctgctc aatcaactct acggccgccg cagcgagcgt
     1261 tcgatcatcg atcccaatca attgctcctg gagttcggct gggaccccgc cgcgcccgac
     1321 gcgttggccg ccgcggcggc cgaggcggaa acgatcgtgc aagaattcac ggtgcggcgc
     1381 gagatccaga agaagtcgaa accgcgcgac gaacgattgc ccgcgcatct cgaacgctac
     1441 gaagtgatcg ccgacgcgtc ggacgaacag aagctgtgcc ccgatcacgg cgagcggcag
     1501 gtgatcggct acgacgtgac ggagacgctg gagttcgagc gcccgaagct caaggtccgc
     1561 gtgaccaagt atgcgaaata cgcttgcgtc ggcaagccgg cgtgcggcat cggccaggcc
     1621 gcgcgtccga acggactggt cgaaggcaac cgctacggca cgagcgtggc ggccgaaatc
     1681 atcgcaggca agttcttcct gcacctgccg ctctatcggc agcaagacca attcgccgcc
     1741 agcggctgga ctcccagtcg ctcgacgctc ttgaacatcg cgaccgcggc gacgacggtg
     1801 ctcgagccgt tggccgacga ctatcgccgc cggctgctgc aaagcggcgg gatcggctgc
     1861 gacgatacga cgttgacgct ggtcacgccc gcgacgatcc ccaaactgga tctgaacgac
     1921 cctcgctcgc ggcgaattca tgaagtgctc agcaaggcgc acgccaaggg gcgcccgagc
     1981 atcacggcgc ggatgtgggg ctaccgttcg ttcgaagcgc cgatcaacgt cttcgacttt
     2041 acggtcagcc gtcaccgcga cggccccgac gaatttttga aagactacgt cggcagcctg
     2101 atgggcgact gttggtcggg ctttcagaag atcgagctgc gcagcgacgc gcggattgtg
     2161 cgcgccgcat gctggtcgca cgcgcggcgg aaggtgttcg aaggtcgcag cagccatccg
     2221 cagcaggcta gcgtgctatt ggctctggcc ggcgcgttgt tcgacatcga ggatcgcggc
     2281 aagctgttgt ccgccgaggg acgccgcgag ttgcgcgagc gcgagtcgcg gccggcactc
     2341 gatcgcatcc gcgcgttcgt ggaccatccc gccacggcgt gcgttctgcc caagagtgcg
     2401 tttggcgagg cgctctctta cctgcgcaac cactgggacg cgctgcaaac gtacgtgtcc
     2461 gacggccgga tgccgatcga caacaacgac gacgagcagt tgatgaggca ggtcgccgtg
     2521 ggcagaaaga actggctgtt cgtcggcagc ctggaggctg gggcgcgagc ggcgacgctg
     2581 atgacgatcg tcagcacggc ggcgcggaac gacctcgacg tgtacgtcta cctgaaggac
     2641 gtgctggacc aattgctggt cggctcgacc gactaccgct cgctgcacgc cgacgtctgg
     2701 aaacaatcgc atcccgaggc ggtgcgctct taccgagccg acgagcgtcg cgacgccgcc
     2761 gaccgtcgcc atcaccgccg cgcccaacgg cgactggccc agcgttccgc cgaccagcct
     2821 tcctagcccc gccggccccg gccccgcggc ccacctggcg ccggtgtacg gttacgcgga
     2881 agcgcgcggc ccaatgcccg cttcgaggct ctctgcgaac tggcggggat taagcctaag
     2941 cgagaccttg aaactgggga cgacgcgccc tgggtgctga aggatctg
//
LOCUS       DASUCJ010000722         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_771917, whole genome shotgun sequence.
ACCESSION   DASUCJ010000722 DASUCJ010000000
VERSION     DASUCJ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771917"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1744)
                     /locus_tag="VFU90_08660"
     CDS             complement(<1..>1744)
                     /locus_tag="VFU90_08660"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5479888.1"
                     /translation="VYRTGDGRPGAHYWQQRADYDLHATLDTANKSLSASMTMRYTNN
                     SPDTLRFIWLQLDMNAGSPDTRFAPLTNPRARQEPGFRAGATIERVAALRAARAGARA
                     TAAPLTWRVNSTMMRVDLDRPLPPHGVLSFVVAWHHQIPLRGRTGRQHFTTGWLYQVA
                     QWYPRLAVYDDVRGWNTDQYIGSSEFYLEYGDFDVTLTVPAVYTVAATGVLRNPLDVL
                     PSVIRARLAAAQHADTIVHIIAGDEVGTPALLPARTGATRTWHYSATNVRDFAWGTAP
                     NFLWDATSWNGILMQTFYPPTVADIWKTAADMNRHAVMIHSRWFHYPWPTAISVEGPV
                     GGMEYPMITFDNAENEKELYYTLAHEHGHEWFPMMVGSDERLYPWMDEGFNTFIDWFS
                     FRSRYPTDTTRIQSLEFGSMRAYQTFLQNYRGIETPIMESQDRSPSGLMSGWNAYGKP
                     AVALHFLREEVVDSNAFDDAFREYVRRWVYKHPQPADFFRTMNNALGEDLSWFWRSWF
                     IRSDHLDQAVDSVVQQDSGTQTLTRVFLSSRLEMVAPVELRITGADSTTRTVKLPVET
                     WLRGSRAVWATITPT"
BASE COUNT          290 a          581 c          576 g          297 t
ORIGIN      
        1 gtcggcgtaa tcgtcgccca gacggcacgc gagccgcgca gccacgtctc gactggcagc
       61 ttcaccgtgc gggtcgtact gtccgcgccg gtaatgcgca gctcgaccgg ggccaccatc
      121 tcgagccggc tcgacaggaa cacgcgcgtc aacgtctggg tccccgaatc ctgctgcacc
      181 accgaatcga ccgcctggtc gaggtggtcg gagcggatga accaggagcg ccagaaccac
      241 gacagatcct cgcccagcgc gttattcatg gtgcggaaga agtcggccgg ctgcgggtgc
      301 ttgtacaccc agcgccgcac gtactcgcgg aacgcgtcgt cgaaggcgtt cgaatcgacg
      361 acttcctcac gcaggaagtg cagcgccacc gccggcttcc catacgcgtt ccagccgctc
      421 atcaagccgc ttggcgagcg gtcctgcgat tccatgatcg gcgtctcgat gccacgatag
      481 ttctgcagga acgtctggta ggcgcgcatc gagccgaatt cgagcgactg aatccgggtc
      541 gtgtccgtcg ggtagcgcga acggaagctg aaccagtcga tgaaggtgtt gaacccctcg
      601 tccatccacg ggtagagccg ctcgtccgac ccgaccatca tcggaaacca ctcgtgcccg
      661 tgctcgtgcg cgagcgtgta gtacagttcc ttttcgttct ccgcattgtc gaaggtgatc
      721 atcgggtatt ccatcccgcc caccggcccc tcgacgctga tcgccgtcgg ccacggataa
      781 tgaaaccacc gcgagtggat catcacggcg tggcggttca tatccgcggc ggtcttccag
      841 atatcggcga cggtaggcgg gtagaacgtc tgcatcagga tgccgttcca gctcgtcgcg
      901 tcccagagga aattcggcgc cgtgccccag gcgaagtcgc gcacgttggt ggcgctgtag
      961 tgccaggtcc gcgtcgcccc ggtccgcgcc ggcaggagtg ccggcgtccc gacctcgtcg
     1021 cctgcgatga tgtggacgat cgtgtcggcg tgctgcgccg cggcgagccg cgcgcggatc
     1081 acgctcggca ggacgtcgag cggattgcgc agcaccccgg tggcggcgac cgtgtacacc
     1141 gccggcacgg tgagggtgac gtcgaagtcg ccgtactcga gataaaactc gctcgacccg
     1201 atgtactgat cggtgttcca gccgcgcacg tcgtcgtaca cggccagccg cggataccac
     1261 tgcgccacct ggtacagcca gccggtcgtg aaatgctgcc ggccggtgcg cccgcggagc
     1321 ggaatctggt ggtgccacgc gacgacgaac gacagcacgc catgcggcgg cagcggccgg
     1381 tcgagatcga cgcgcatcat cgtactgttg acgcgccagg tgagcggcgc cgcggtggca
     1441 cgtgctcccg cacgcgccgc ccgcaacgcc gcgacgcgtt cgatcgtcgc cccggcacga
     1501 aatcctggtt cctgccgggc gcgcgggttg gtgagcgggg cgaaccgcgt gtcgggagag
     1561 ccggcgttca tgtcgagctg cagccagatg aaccgcagcg tatcgggcga gttgttcgtg
     1621 tatcgcatcg tcatcgacgc gctgagcgac ttgttcgccg tgtccagcgt cgcgtgcaga
     1681 tcgtaatccg cgcgctgctg ccagtaatga gcgccgggcc gtccgtcacc ggtcctgtac
     1741 acgt
//
LOCUS       DASUCJ010000723         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1291404, whole genome shotgun sequence.
ACCESSION   DASUCJ010000723 DASUCJ010000000
VERSION     DASUCJ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291404"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VFU90_08665"
     CDS             <1..1500
                     /locus_tag="VFU90_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5479889.1"
                     /translation="GTVAILQPQLASLDVRTAGGRADLVAHGGAPLQPRVVTQNAGAI
                     VYEFAGVGTPLTGTRSVNAGGIRTLQIAQTGTVRDPKTVVTVSLAPGAVHDVPRSNDD
                     RDVVISFASSPAAASAPAAQSQTATAPAPGGPNAPVSADGNAAAPSGAAVTGVTVTPS
                     DDGYAVTIAITGNAAFEWHRLREPDNRFWIDVKGAALQGPPIDASEPDPVGALRVRQI
                     DPSTVRVALSLAGPKALMVSPTSTGLSVDVGRSDIADVPRAGNGMVGAAPAGVQTALV
                     TPAPIDSSGTAGDSDSAWKFGPRGSTYVPRNPRLIVIDPGHGGSDRGTSRHGTDEASL
                     TLDMSRRLRDILVARGWQVRLTHDADVDVYQPDDSAHDELQARVDVANNAGARLFLSI
                     HVNAYINSGPYGTTEYVSKPSDVALAREVESQLSSDGTKDDGIVKSHLYVTLHTQMPA
                     VLVETAFISNPSDYALLTSAAWRQKVAQSIADGIDQYNREYPVSNDPSQ"
     gene            1508..>2088
                     /gene="murI"
                     /locus_tag="VFU90_08670"
     CDS             1508..>2088
                     /gene="murI"
                     /locus_tag="VFU90_08670"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5479890.1"
                     /translation="MIGLFDSGLGGLTVIRRIRERLPSADIVYFADQAHVPYGDREPE
                     ELVELLVANLAWIDAAGADAIVMACNTSCAIGESYGWPSTRAPILDIIDSAATAVQRA
                     GYRRIGVIATAATVSAGSYGRTLRSAVPGAEVWEVAAPRLVPMIEAGRITGSEPRAAV
                     AAVCGRLPKDLDAVIYGCTHYPVLEPQFAAAFGR"
BASE COUNT          336 a          726 c          713 g          313 t
ORIGIN      
        1 ggcaccgtcg caatcttgca gccgcagctg gcgtcgctcg acgtgcgcac cgcgggtggc
       61 cgcgcggatt tggtggcgca cggcggagcg ccgctgcaac cgcgcgtcgt gacgcagaac
      121 gccggcgcga tcgtctacga atttgccggc gtcggcacgc cgctcaccgg cacgcgcagc
      181 gtgaacgccg gcggaatccg cacgctgcag atcgcccaaa ccggaacggt gcgcgatccc
      241 aagacggtcg tcacggtttc gctcgcgccc ggcgcggtcc acgacgtacc gcgcagcaac
      301 gacgatcgcg acgtcgtgat ttcgtttgcc tcgtcgccgg ccgccgcgag cgcgccggcg
      361 gcgcagtcgc aaacggcaac cgcgccggcg ccgggagggc ccaacgcgcc ggtctccgcc
      421 gacggcaacg ccgcggcgcc cagcggggcg gccgtcaccg gcgttaccgt aacgccgtcg
      481 gacgacggct acgccgtgac gattgcgata acgggcaacg ccgccttcga gtggcaccgg
      541 ctgcgcgaac cggacaaccg cttctggatc gacgtcaaag gcgctgcgtt acaggggccg
      601 ccgatcgatg cgagcgagcc cgacccggtc ggcgcgctgc gggtccgcca aatcgacccg
      661 agcaccgtgc gcgtcgcgct ctcgctggcc ggtccgaaag cgctgatggt ttcgccgacc
      721 tcgacgggct tgagcgtcga cgtcgggcga tccgacatcg ccgacgttcc tcgagccggg
      781 aacggcatgg tgggcgctgc gcccgccggc gtccagacgg cgctcgtcac gccggctccg
      841 atcgacagct cgggaaccgc cggcgactcc gattcggcgt ggaagttcgg accgcgcggc
      901 agtacgtacg tgccgcggaa cccgcgtctc atcgtgatcg atcccggtca cggcggaagc
      961 gatcgcggca cgtcccgtca cggcaccgac gaggcgtcgc tcaccctcga tatgtcgcgg
     1021 cggctgcgcg acattctcgt cgcccgcggc tggcaggttc ggctgacgca cgacgccgac
     1081 gtcgacgttt accagcccga cgacagcgca cacgacgagc tgcaggcgcg cgtcgacgtc
     1141 gccaacaatg ccggcgcgcg gctgttcttg agcatccacg tcaacgcgta catcaactcc
     1201 ggtccctacg gaacgaccga atacgtttcc aagccaagcg acgtcgcgct ggcgcgcgag
     1261 gtcgagagcc agctgtcgtc ggacggtacg aaagacgacg gaatcgtcaa aagccacctt
     1321 tacgtcacgc tgcacacgca gatgccggcg gtgctggtgg agacggcgtt catttccaat
     1381 ccgagcgact acgcgctgct gacgtcggcg gcttggcgcc aaaaggtagc gcaatcgatt
     1441 gccgacggaa tcgatcagta caaccgggag tatcccgtgt ccaacgatcc atctcaataa
     1501 ctcgccgatg atcggactct tcgattcggg cctgggcggc ctcaccgtca tccggcgtat
     1561 tcgcgagcgg ctcccgtcgg cggatatcgt ctattttgcc gatcaggcgc acgttccgta
     1621 cggcgatcgc gagccggagg aactcgtcga gctgctggta gcgaacttag cgtggatcga
     1681 cgccgcgggg gccgacgcga tcgtaatggc gtgcaacacg tcctgcgcca tcggcgagag
     1741 ctacggttgg ccgtcgaccc gcgcgccgat cctcgacatc atcgattcgg cggctacggc
     1801 cgtgcagcgg gccgggtatc gccgcatcgg cgtgatcgcg acggcggcga cggtcagcgc
     1861 gggctcgtac gggcgcacgc tgcgctccgc cgttccgggc gcggaggtct gggaagtcgc
     1921 ggcgccgcgg ctggtaccga tgatcgaagc cggccgcatc accgggagcg aaccgcgcgc
     1981 ggcggtcgcc gcggtgtgcg ggcgtttgcc gaaagatctc gatgccgtca tttacggatg
     2041 cacccactat ccggtgctcg agccgcagtt cgcggccgcc ttcggccg
//
LOCUS       DASUCJ010000724         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_328214, whole genome shotgun sequence.
ACCESSION   DASUCJ010000724 DASUCJ010000000
VERSION     DASUCJ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328214"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /gene="lepB"
                     /locus_tag="VFU90_08675"
     CDS             <1..536
                     /gene="lepB"
                     /locus_tag="VFU90_08675"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5479891.1"
                     /translation="VSMVPTLQVRDVLLVDEVAYRLHPPQTGDIAVFVPPVEAHGDDF
                     VKRVIGTPGDTIRIANGVVYRNGTALDEPYENQPPRYDLRIERYGIYVDGTALDPHVA
                     DVPPKSMWQSPDRVPRGFYFMLGDNRNYSDDSHVWGFAQTSGAFAAGPLAGKRRAGFA
                     GRAFLILWPFGRLRILH"
     gene            565..>1219
                     /gene="lepB"
                     /locus_tag="VFU90_08680"
     CDS             565..>1219
                     /gene="lepB"
                     /locus_tag="VFU90_08680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5479892.1"
                     /translation="MTPPELLAIVAVIAIARIVLSLRPVVAGSNGRSTSIAREFLDPF
                     IIAGLAAWVLITFVARTYYIPSGSMLPTLQIHDVLLVDKFEYRFHEPNRGDIVVFPPP
                     IPTPDDFIKRVIGRPGDTLAVRNGTVYVNGVALKEPYVEQRPAYDLEIRDYGIYVKSD
                     GSDWERLDPQSANVPPKTAWTAPDRIPPNCFMMFGDNRNESEDSHMWGFAQVAGRFAT
                     "
BASE COUNT          212 a          410 c          367 g          230 t
ORIGIN      
        1 cggtttcgat ggtgccgacc ctgcaagtgc gcgacgtgct gctagtcgac gaagtcgcct
       61 accggctcca tcctccgcaa accggcgata tcgcggtttt cgtgccgccg gtggaagcgc
      121 acggcgacga cttcgtcaag cgcgtcatcg ggactcccgg cgacacgatc cgcatcgcca
      181 acggtgtggt ctatcgcaac ggtacggcgc tcgacgagcc gtacgagaac cagccgccgc
      241 gatacgatct gaggatcgaa cgctacggca tttacgtcga cgggactgcg ctcgacccgc
      301 acgtcgccga cgtcccgccc aaatcgatgt ggcagtcgcc cgaccgggtt ccccgcgggt
      361 tttacttcat gctgggcgac aaccggaact attcggacga ttcgcacgtc tggggtttcg
      421 cacaaaccag cggtgcgttc gcggccggac cgctcgccgg caagcggcgt gcgggcttcg
      481 ccgggcgggc cttcttgatc ttatggccgt tcggccgctt gcgcattctt cactgacagc
      541 atcacggaaa gcgacgcttc tgtcatgacg cctcccgaac tactcgccat cgtcgccgtc
      601 atcgcgatcg cgcgcatcgt cctttcgctg cgcccggtcg tcgccggctc caacgggcga
      661 agcacctcga tcgcgcgcga gtttctcgac cccttcatca ttgcggggct ggcggcttgg
      721 gtgctgatca cgttcgtcgc gcgaacctac tatattccct cgggttcgat gctgccgacg
      781 ctgcagattc acgacgtgct gctggtcgat aagttcgagt accgatttca cgagcccaac
      841 cgcggcgaca tcgtcgtatt tccgccgccg attccgacgc ccgacgattt catcaaacgc
      901 gtgatcggcc ggcccggcga cacgctcgcg gtgcgaaatg ggacggtata cgtcaacggc
      961 gtcgcgctga aagaaccgta cgtcgagcag cgtcccgctt acgatctcga gatccgcgac
     1021 tacgggatct acgtcaagag cgacggaagc gactgggaac ggctcgatcc ccaatccgcc
     1081 aacgtccctc ccaagacggc gtggaccgcg ccggaccgca ttccgccgaa ctgtttcatg
     1141 atgttcggcg acaatcgcaa cgagtcggaa gactcgcata tgtggggatt cgcgcaggtc
     1201 gccgggcgct tcgcgaccg
//
LOCUS       DASUCJ010000725         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_634156, whole genome shotgun sequence.
ACCESSION   DASUCJ010000725 DASUCJ010000000
VERSION     DASUCJ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634156"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFU90_08685"
     CDS             complement(<1..660)
                     /locus_tag="VFU90_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5479893.1"
                     /translation="MARIIALVNQKGGVGKSTTAVNLGAALAVLGKRVLVVDADPQGN
                     TTTGLGIDKTQLRRDVYDVLLQEASADDVIVPTELENLWLVPATINLAGAEIELVAAL
                     SRETRLRQAIAPVAARYDYVFIDCPPSLGLLTINALTTAEECIIPVQAEYYALEGLSQ
                     LTSVVRRVREALNPQLHVSGVLVTMFDGRTRLAAEVLEELVKFFPEQMFKTQIPRNVR
                     LS"
     gene            complement(661..>1560)
                     /locus_tag="VFU90_08690"
     CDS             complement(661..>1560)
                     /locus_tag="VFU90_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5479894.1"
                     /translation="QFAARFEYAVTPRTLAAITAAAPLAATVSAERVADELSKLLLQA
                     PRPSIGLELLRSTGVLAQIWPELLEGVGVEQNEWHAYDVWYHSMATVDASPPGDLVLR
                     LAALLHDVGKPRTKEGPHFYRHEIAGAEMVRAMLGRLRYSNDTVERTALLVRQHMFAA
                     DPEMTGAAVRRFIRRVGTESIEHLFVLRAADVAGSGLPKRGDHNERFADRVRAEIAKK
                     PPFSVKDLRVSGADVIAALVRRGDASGDFRGDARVGRALQWLFEQVTDAPERNERTSL
                     LGLLEQYLDAHPRGVHLSEKLGS"
BASE COUNT          262 a          512 c          517 g          269 t
ORIGIN      
        1 cgataggcga acgttccgcg gtatctgcgt cttgaacatt tgttccggaa agaacttgac
       61 gagctcttcg agcacttcgg cggcgagccg cgttcgcccg tcgaacatcg tgacgaggac
      121 gccgctcacg tgcagctgcg gattgagcgc ctcgcgcacg cgccgaacga ccgacgtgag
      181 ctgcgagagc ccttcgagtg cgtagtattc ggcttgcacc gggatgatgc actcttcggc
      241 ggtcgtcagc gcattgatcg tgagcaatcc gagcgacggc gggcagtcga tgaaaacgta
      301 gtcgtagcgg gcggcgaccg gcgcgatcgc ttgccgcagg cgcgtttcgc gcgacagggc
      361 ggcgacgagc tcgatctccg cgcccgctag attgatggtg gcgggcacca gccagaggtt
      421 ttcgagctcg gtcggaacga tgacgtcgtc ggcgctcgct tcctgcagca gcacgtcgta
      481 aacgtcgcgc cgcagctgcg ttttgtcgat ccccaatccg gtggtggtgt tgccctgcgg
      541 atccgcgtcg acgacgagca cgcgctttcc gaggacggcc aaagccgcgc cgagattcac
      601 cgccgtcgta ctctttccga cgccgccttt ttgattgacg agggcgatga tgcgcgccag
      661 ttacgacccc aacttctcgg aaagatgcac gccgcgtggg tgcgcgtcca agtactgctc
      721 gagtaggccg agcagcgaag tgcgctcgtt acgctcgggt gcgtccgtga cctgctcgaa
      781 aagccactgc agcgcgcgtc cgacgcgcgc gtcgccgcga aaatcgccgg acgcatcgcc
      841 gcggcgcacg agcgcggcga tgacgtcggc gcccgatacg cgtagatctt tcaccgaaaa
      901 cggcggtttt ttcgcaattt ccgcgcgcac gcgatcggcg aaacgctcgt tgtggtcgcc
      961 gcgtttaggt aagcccgagc cggccacgtc ggctgcccgt aatacgaaca gatgttcgat
     1021 actttcggtc ccgacccgcc ggatgaagcg ccgcaccgcg gcgccggtca tctcgggatc
     1081 ggccgcaaac atgtgctggc gcaccagcag cgcggtccgc tcgacggtat cgttcgagta
     1141 ccgcagccgt ccgagcatcg cacggaccat ttcggcgccg gcgatctcgt ggcggtaaaa
     1201 atgcgggccc tccttcgtgc gcggcttgcc gacgtcgtgg agcagagcag ccagcctgag
     1261 gaccagatcg cccggcggcg acgcatcgac ggtggccatc gagtggtacc agacgtcgta
     1321 ggcgtgccac tcgttttgtt cgacgccgac cccttcgagc agctcgggcc agatctgcgc
     1381 gagcacgccg gtcgaccgga ggagctcgag cccgatcgaa ggccgcggcg cctggagcag
     1441 cagcttggag agctcgtcgg cgactcgctc tgccgaaacc gtcgccgcca gcggcgctgc
     1501 cgcggtaatc gccgccagcg tcctcggcgt gaccgcatac tcgaaccggg ccgcgaactg
//
LOCUS       DASUCJ010000726         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1291410, whole genome shotgun sequence.
ACCESSION   DASUCJ010000726 DASUCJ010000000
VERSION     DASUCJ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291410"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFU90_08695"
     CDS             complement(<1..719)
                     /locus_tag="VFU90_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5479895.1"
                     /translation="MGAPSLPPGRYFRMHIIGYFEGIDSERGIAWRCADSFSLRDFLR
                     LSNREKVPDHSWLSRTRSRLAHEAHETVFGWVLKLVAERGLVKGERIGVDGSTMEANA
                     ALRTIVRRDSGESYRAMLTRMAQESGIETPTAEDLARFDRKRKGKTLSNADWKSPTDR
                     DARIAKMKDGTTHLAYKPEHAVDLDTGVVVAAPIHPANEGDTATLSPTLDAAARNLDA
                     VGLAPTKEEPCVVAADKGYHAR"
BASE COUNT          170 a          409 c          335 g          187 t
ORIGIN      
        1 cgcgcgtgat agcccttgtc ggcggccacg acgcagggtt cctccttggt cggcgccagt
       61 ccgaccgcgt cgaggttgcg cgcagccgcg tccagcgtcg gcgaaagcgt cgccgtatcg
      121 ccttcgttgg ccggatggat cggcgcggcc accacgacgc cggtgtcgag atcgaccgca
      181 tgctccggct tgtaggccag atgcgtcgtc ccgtccttca tcttcgcgat cctggcgtcg
      241 cggtcggtcg ggctcttcca gtcggcgttc gacagcgtct tgcccttgcg cttgcggtcg
      301 aagcgcgcca ggtcctcggc cgtcggcgtc tcgatcccgc tctcttgcgc catccgcgtc
      361 agcatcgcgc gatagctctc accgctgtcg cgccgcacga tcgtgcgcaa cgcggcgttg
      421 gcctccatcg tcgagccgtc gacgccgatc cgctcgccct tcaccaggcc gcgctcggcc
      481 accagcttca gcacccagcc gaacaccgtc tcgtgcgcct cgtgcgccag acgcgagcgc
      541 gtgcgcgaca gccaggaatg gtcgggaacc ttctcccggt tcgacagccg caggaaatcg
      601 cgcagcgaaa acgagtccgc gcaacgccac gcgatgccgc gctcgctgtc gatcccctcg
      661 aaatagccga ttatgtgcat ccggaagtac cgccccgggg gaagcgacgg cgcgcccatc
      721 cgcggcgcgt agtagggctt gcacacctcc tccacgaacc cgtcgaatcc ggcttcccga
      781 agcaggtcct gcaagcgatc atagaatgcg tgccccggcg aacgcggcat atccgcccac
      841 gtcgccatca ggtcatcctg gaccgcttcc gatcgcccca tcgccatcgc tgcttcccgc
      901 cacgactcaa cgaaaagacg gaatcagcga tgatcgactt cgtcaacggg ctcctaaccc
      961 cggcgctggc gcgtgttctg cagacgacgt cccaacttgc cgagggtgcg atcggctggg
     1021 agttcgcggg ccgcctccac cacccgatgc gggcggccgg ggcggcttgg ctcgcctaca
     1081 tcgatcaact gatcgaagac c
//
LOCUS       DASUCJ010000727         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_404514, whole genome shotgun sequence.
ACCESSION   DASUCJ010000727 DASUCJ010000000
VERSION     DASUCJ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404514"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFU90_08700"
     CDS             complement(<1..181)
                     /locus_tag="VFU90_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEU5479896.1"
                     /translation="MKEEDPELSRAFAAREAWAYEAAYRLHGRTLYAAAFNVLRTAHD
                     AQDCVHDVLLRLWQRG"
     gene            complement(183..1055)
                     /locus_tag="VFU90_08705"
     CDS             complement(183..1055)
                     /locus_tag="VFU90_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5479897.1"
                     /translation="MNRRNFLSHVGWTGAGIAYALTAQGVVRAADPPAGPFFVQISDS
                     HIGFAQAANPDVAATLSATVAAINALPSRPQFVVHTGDVTHLAKAGQFDDAKQILAAL
                     RAPLIVLPGEHDVLGSTPARFLAAFPPPSGTDGWFSWEAGGVHYVALINVFDFEQMGV
                     LGNEQLTWLERDLAPLPRSRPVVVFAHVPLYALYPEWGWTTGDGAKALALLARFDAVT
                     VLNGHIHQVIEHVEGNIRFATAHATAYPQPAPGTAPKPGPLTLPRDQLLHVLGYRTVQ
                     LHGGAGALDDVTLG"
     gene            complement(1052..1393)
                     /locus_tag="VFU90_08710"
     CDS             complement(1052..1393)
                     /locus_tag="VFU90_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEU5479898.1"
                     /translation="MNLLIFAAVAALAPAALAANPAAATVHIRDFAFVPPTLTVVPGT
                     TVRFVNDDTEAHTVTASDRSFDSGGLDTGDSWTYRFASPGKFAYFCALHPYMHGVVTV
                     TAAPAHGSSAR"
     gene            complement(1430..1981)
                     /locus_tag="VFU90_08715"
     CDS             complement(1430..1981)
                     /locus_tag="VFU90_08715"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5479899.1"
                     /translation="MMLRRFAIVAAAAAFAATAPALAALSVGSKAPDFTLQATQGGNV
                     FTFDLASALKKGPVVLYFYPAAFTTGCTIEAHDFADAIDQYHALGATVIGVSHDPLDK
                     LQKFSVSECRSKFAVAADTDQTVEKAYDAVLAQHPQYANRTSYVIAPDGTIVYTYTNL
                     DPSQHVENTLTALKTWETDHPNK"
     gene            2065..2514
                     /locus_tag="VFU90_08720"
     CDS             2065..2514
                     /locus_tag="VFU90_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HEU5479900.1"
                     /translation="MEPSGRPRDAESRVVWIVVGVVLAVFVVTGTLVGLAVAFGFTLF
                     RLMDRTPAHVCGLAAVRSSPIAAALVGTPIEQRGFTGGSSSSENGELNERITFTVAGP
                     RGSAFVRAEGRRSPLDSHLEVRIGRNGRSAIVYSGPFDCPELHAPAR"
     gene            complement(2515..3051)
                     /locus_tag="VFU90_08725"
     CDS             complement(2515..3051)
                     /locus_tag="VFU90_08725"
                     /inference="COORDINATES: protein motif:HMM:NF027286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEU5479901.1"
                     /translation="MSADQVLAAASIGIAIVAMASSSILLVRQNQQLEHERNAMAILD
                     AISRLTDPAAMVAFESLEGIDARYPDDEAILTRFDDSEDDRNLLLVAQYFETVACLAR
                     RKVLDASLIADAVGFMLRTRWETIRPFVLRLRRLRGNETIFEHFEWIARYSAWWRTIA
                     PPLKNPNYDPGQFAGLER"
     gene            complement(3065..>3603)
                     /locus_tag="VFU90_08730"
     CDS             complement(3065..>3603)
                     /locus_tag="VFU90_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5479902.1"
                     /translation="MGWRASDTRELSFVDARVPEDHLLGPRGEGYKQFLTILDGGRIS
                     VAALSIGLALGAYDEALAYSKERHAFGRPISKFQAISFKLVDMLTEIEHARLMMLRAA
                     WEKDRGRDYVQSACFAKLFAGELSHRVVNEALQIHGGYGFMDEYPISRMYRDQKINEI
                     GEGTNEVQRVVLARLMGL"
BASE COUNT          587 a         1187 c         1208 g          621 t
ORIGIN      
        1 cgccgcgctg ccacagccgc agcagcacgt cgtgcacgca atcttgcgcg tcgtgcgccg
       61 tgcgcagcac gttgaacgca gccgcgtaga gcgtgcgccc gtgcaaccgg tacgccgcct
      121 cgtacgccca cgcttcgcgc gccgcgaacg cccgcgatag ctccggatcc tcttctttca
      181 acctacccca gcgtcacgtc gtcgagcgcg ccggctcctc catggagctg caccgtgcgg
      241 tagcccaaca cgtgcagaag ctgatcgcgc ggcagggtca gcggtcccgg cttcggggcg
      301 gttcccggcg ccggctgcgg gtacgcggtg gcgtgggcgg tcgcgaagcg gatgttgcct
      361 tcgacgtgct cgatgacttg gtggatgtgg ccgttgagca ccgtcacggc gtcgaaacgc
      421 gccagcagcg ccagcgcctt ggccccgtcg ccggtggtcc acccccactc cggatagagt
      481 gcgtacaacg gcacgtgggc gaagacgacg accggccgcg agcgcggaag cggcgccagg
      541 tcgcgctcca gccaggtcag ctgctcgttt ccgagcactc ccatctgctc gaaatcgaag
      601 acgttgatca gcgcgacgta gtgcacgccg ccggcctccc acgaaaacca gccgtccgtt
      661 ccggacggcg gcggaaaggc ggcgagaaaa cgcgccggcg tggagccgag cacgtcgtgc
      721 tcgccgggca gcacgatcag cggagcgcgc agcgcggcga ggatctgctt ggcatcgtcg
      781 aactgtccgg ccttcgcgag gtgcgtcacg tcgccggtgt gcaccacgaa ctgcggccgc
      841 gacggcagcg cgttgatcgc ggcgacggtg gcgctgaggg tcgccgccac gtccggattc
      901 gccgcttgcg cgaacccgat gtgactgtcg ctgatctgca cgaaaaacgg accggccggc
      961 ggatccgcag cgcgtacgac gccctgcgcc gtcagcgcgt aggcgattcc cgctccggtc
     1021 cagccgacgt gcgacaaaaa atttcgccgg ttcatcgggc ggagctcccg tgcgcgggcg
     1081 ccgcagtcac cgtcacgacg ccgtgcatgt acgggtggag cgcacagaag tacgcgaact
     1141 tcccgggcga cgcaaaccgg tacgtccacg agtctcccgt atcgagtccg cccgaatcga
     1201 acgaccggtc cgacgcggtg acggtatggg cttcggtgtc gtcgttgacg aagcgcacgg
     1261 tcgtcccggg caccaccgtc agggtcgggg gtacgaaggc gaaatcccgg atatggaccg
     1321 ttgcggcggc cgggttagcc gccagagcgg cgggcgcgag tgccgcgacc gccgcgaaaa
     1381 tcaagaggtt catgccctct ctatgcgcga gcgggccgaa cggttcggtc tatttgttgg
     1441 ggtggtcggt ttcccaggtt ttcagcgcgg tcagggtgtt ctcgacgtgc tgcgacggat
     1501 cgaggttggt gtaggtgtag acgatcgttc cgtcgggtgc gatgacgtac gacgtacggt
     1561 tggcgtactg cggatgctgc gcgagcaccg cgtcgtaggc cttctcgacc gtttgatcgg
     1621 tgtcggccgc gacggcaaac ttgctgcggc attcgcttac cgagaatttc tgcagtttgt
     1681 cgagcggatc gtgcgagacg ccgatcaccg tggcgccgag cgcgtggtac tggtcgatcg
     1741 cgtcggcgaa gtcgtgcgct tcgatcgtgc agccggtcgt gaaagcggcc ggataaaagt
     1801 agagaacgac cgggcccttt ttcagcgccg aggcgagatc gaaggtaaag acgttcccgc
     1861 cctgcgtcgc ctgcagcgtg aagtcggggg ctttgctgcc gaccgaaagc gccgccagcg
     1921 cgggcgcggt cgctgcaaaa gccgctgcgg cggccactat ggcaaaccgg cgtagcatca
     1981 tggatttcac tcctccggct cacacgatac cacggggcgg caaacggttt catgaaagct
     2041 ggacggggcg cgtagaatcg tcctatggaa ccgagcggcc gcccccgcga cgcggagtcg
     2101 cgcgtcgtct ggatagtggt cggcgtggta ctcgccgtct tcgtcgtgac cggtacgctg
     2161 gtcgggctcg cggtcgcttt cggcttcacc ctcttccgtc tcatggatcg cacgccggcg
     2221 cacgtgtgcg ggctcgcggc ggtgcgctcc agcccgatcg ccgccgcgct cgtcggcacg
     2281 ccgatcgaac agcgcggatt taccggcggc tcctcgtcgt ccgaaaacgg cgagctcaac
     2341 gaacggatca ccttcaccgt cgccgggccg cgcggcagcg cgttcgtgcg tgccgagggc
     2401 cggcgctcgc cgctggattc gcatctcgag gtgcgcatcg ggcggaacgg gcgcagcgcg
     2461 atcgtctatt ccggtccgtt cgactgtccg gagctgcacg cgcccgcgcg ctagctagcg
     2521 ctccaaaccg gcaaactgcc cgggatcgta gtttgggttc ttgagaggcg gcgcgatggt
     2581 tcgccaccac gcgctgtacc gcgcgatcca ttcgaagtgc tcgaagatcg tctcgttccc
     2641 gcgaaggcgg cgcaggcgca gcacgaacgg ccggatcgtt tcccaacggg ttcgcaacat
     2701 gaaacccacg gcgtcggcta tcaggctcgc gtcgagcacc ttgcgccgcg cgaggcacgc
     2761 gacggtttcg aaatactgcg cgaccagcag caggttccgg tcgtcctcgg agtcgtcgaa
     2821 acgcgtgagg atcgcttcgt cgtcgggata gcgcgcgtcg atcccctcga ggctttcgaa
     2881 ggcgaccatc gccgccgggt cggtaagccg gctgatcgca tccaggatcg ccatcgcgtt
     2941 gcgctcgtgc tcgagctgtt gattctgccg aacgagcaga atcgacgacg acgccatggc
     3001 gacgattgcg attccgatcg aggcggcggc gagaacctga tccgcgctca tcgccgcgag
     3061 cgcttcaaag ccccatcaag cgcgcgagca cgactcgctg tacctcgttc gtgccttcgc
     3121 cgatctcgtt gatcttttgg tcgcgataca tgcgcgaaat cggatattcg tccatgaatc
     3181 cgtaaccgcc gtggatctgc agcgcttcgt tgaccacccg gtgcgacaac tcgccggcga
     3241 acagtttggc aaagcaagcc gactgtacgt agtcgcggcc ccggtctttc tcccacgcgg
     3301 cgcgcagcat catcaaccgg gcgtgctcga tttccgtcag catgtcgacg agcttgaacg
     3361 agatcgcctg aaacttgctg atcgggcgcc cgaacgcgtg ccgttctttc gaataggcga
     3421 gcgcttcgtc gtacgctccc agcgcgagcc cgatcgacag cgccgccacc gagatgcggc
     3481 cgccgtcgag gatcgtcaag aactgtttgt agccctcgcc tcgcgggccg agcaagtggt
     3541 cttcgggcac ccgcgcatcc acgaacgaca gctcgcgggt gtcggatgcg cgccagccca
     3601 tct
//
LOCUS       DASUCJ010000728         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_603520, whole genome shotgun sequence.
ACCESSION   DASUCJ010000728 DASUCJ010000000
VERSION     DASUCJ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603520"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFU90_08735"
     CDS             complement(<1..757)
                     /locus_tag="VFU90_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEU5479903.1"
                     /translation="MDAWLESLPAAGAGALVVGGFLILTIVIGTLVGKFAPRRVRIVH
                     NDLAGFILAVIGVIYAVLLAFVTIGVWERYQLAESRTYDEAGMLAVAYRDADAFPQGH
                     QLRAAIRAYVEAVITDEWPKMRRGGSSDEADRWSERADRIVRSLSVKTSDQQDVHNQL
                     LAALNSAQTDRDARLSEDASGINGVMWFVLMAGAVVTVGFTFLFGFRNSAMQHLMTGS
                     LAALIGLVLFLAVALNYPYQGSISVAPEAFHNAL"
     gene            complement(769..>1412)
                     /locus_tag="VFU90_08740"
     CDS             complement(769..>1412)
                     /locus_tag="VFU90_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5479904.1"
                     /translation="AGIALAVATLWSATGCASSIQHWIVATRVHQGEAALDHGNVRDA
                     ELAYRLALRIDPADPRARAGYVRAAAGLAQSQYSKGNFDSALATIKDGLAVDPESVRM
                     AAMERTIEQARLKREIVISNYPTYREAGLQLQRAYEQLNSTNKLLLASLRRFGYTFDA
                     NDLTTAIKRSYEMQLDIVKNGNHLVLYRQLVTSGVPEAESGSATGSTSLLPLP"
BASE COUNT          257 a          468 c          444 g          243 t
ORIGIN      
        1 ggagcgcgtt gtgaaacgcc tcgggcgcta ccgaaatact cccttgatac ggatagttga
       61 gcgccaccgc caagaacagt accaacccaa tcaacgccgc cagcgaaccc gtcatcaggt
      121 gctgcatcgc gctgttccgg aagccaaaaa ggaacgtgaa gccgacggtc acgaccgcgc
      181 cggccatcag cacgaaccac atgaccccgt tgatgccgct ggcgtcttcc gacaggcggg
      241 cgtcacggtc ggtttgcgcg ctattgagcg ccgcgagcag ctgattgtga acgtcttgct
      301 gatcggaagt ttttaccgaa agcgatcgca cgatccggtc ggcccgttcg ctccaccgat
      361 cggcttcatc ggagctgccg ccccgccgca tcttcggcca ctcgtcggtg atgaccgcct
      421 cgacgtacgc gcggatggcg gcgcgcagct ggtggccttg cggaaacgca tcggcgtcgc
      481 ggtacgccac cgcgagcatc ccggcctcgt cgtacgttcg cgactcggcc agctggtagc
      541 gctcccagac gccgatcgtt acgaacgcca gcagaacggc gtagatcacg ccgatgaccg
      601 ccaagatgaa ccccgctaaa tcgttgtgca cgatccgcac gcgccgcggc gcaaacttgc
      661 cgacgagcgt accgattaca atcgtgagga tgaggaaccc cccgaccacg agcgcgccgg
      721 cgccggcggc gggaagcgac tcgagccacg cgtccatttt tatgaaccct acggcagcgg
      781 cagcagcgac gtactgccgg tggccgaacc cgattcggct tcgggaacgc ccgacgtcac
      841 gagctggcga tagagcacca gatgattacc gttcttgacg atgtcgagct gcatctcgta
      901 gctgcgtttg atggccgtcg tgagatcgtt ggcgtcgaac gtgtacccga accgccgcag
      961 actggccagc agcagtttgt tggtgctatt gagctgttcg taggcgcgct gcagctgcag
     1021 gccggcctcg cggtaggtcg gatagttcga gatgacgatc tcgcgtttga ggcgcgcctg
     1081 ctcgatcgtt cgctccatcg cggccatccg cacgctctcc ggatctaccg ccaagccatc
     1141 cttgatggtc gccagggcgg aatcgaagtt gcccttggaa tactgcgact gcgcgagtcc
     1201 ggccgcggcg cgtacgtaac ccgcgcgcgc ccgcggatcc gcggggtcga tccgcagcgc
     1261 caggcgatag gcgagctcgg cgtcgcgaac attgccgtgg tcgagcgccg cttccccttg
     1321 atgcacgcgc gtcgcgacga tccagtgctg aatcgagctc gcgcagccgg tcgcgctcca
     1381 cagagtcgca acggcgagtg ctatgccggc ga
//
LOCUS       DASUCJ010000729         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_374801, whole genome shotgun sequence.
ACCESSION   DASUCJ010000729 DASUCJ010000000
VERSION     DASUCJ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374801"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFU90_08745"
     CDS             complement(<1..237)
                     /locus_tag="VFU90_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479905.1"
                     /translation="MPARRLVAAAIALGAVALPSPGLRAETQLDSQYVLQRYALAIDA
                     APAPKYVVFTYAVSQLGPSNLEQRHTIYRSGNDVR"
     gene            complement(240..>1390)
                     /locus_tag="VFU90_08750"
     CDS             complement(240..>1390)
                     /locus_tag="VFU90_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5479906.1"
                     /translation="LDSSSFYAERGGQIGDRGILTAGDGTFEVRDTQYLGEAIAHHGI
                     VREGRIAAGERVRTSVYDWWRREIRRHHTSAHLLQRALKDVLGEEVNQAGSWVGIDRM
                     RFDFRWPGGALTGEQKRAVTRRVNEMIRDDSHLVTRELPLEQAKRTGAIWMAGENYGE
                     SIRVVTAGPSVEFCGGTHSHSTGELGLFVILSESSIGSGIRRIESCVSQAAEDFVDRQ
                     TDLVNGLSSSLATSPEELGERVGKLQRDVRDLQTALGQLKARMAAADAQTYVENAQRR
                     GDRAFVGAVVPEAGSEALRHLAQAIRSRLRSGAIALIGVDGNDVSVLVSASDDLVKAG
                     VHAGELVKLAAPLIGGKGGGQPGQAQGGGKNPAGAQAALDAVRAAVLG"
BASE COUNT          210 a          498 c          450 g          232 t
ORIGIN      
        1 gcgcacgtcg ttgccgctgc gatagatcgt atggcgctgt tcgagattgc tcggacccaa
       61 ttgcgaaacg gcgtacgtga agacgacgta tttcggggcc ggcgcggcgt cgatcgcgag
      121 cgcgtagcgc tgcaggacgt attgggaatc gagctgcgtt tcggcccgca ggcccggcga
      181 cggcagcgcg accgcgccca gcgcgatcgc cgccgcaacg agccggcgcg ccggcacgct
      241 caccccagga ccgccgcgcg cacggcgtcc agcgccgctt gcgcgccggc cgggttcttc
      301 ccgccgcctt gcgcctgtcc cggctggccg ccgcccttcc cgccgatcag cggcgccgcc
      361 agtttgacga gctcgccggc gtgcacgccg gcctttacga gatcgtcgct cgcgctcacc
      421 agcacgctga cgtcgttccc gtcgacgccg atcagcgcga tcgcgccgct gcgcagccgg
      481 ctgcgaatgg cttgcgccag atgccgaagc gcttcgctgc cggcttcggg aacgacggcg
      541 ccgacgaacg cccggtcgcc gcggcgctgc gcgttctcta cgtacgtctg cgcgtcggcg
      601 gccgccatcc gcgctttcag ctggccgagc gccgtctgca gatcgcgaac gtcgcgctgc
      661 agcttcccga cgcgttcccc aagctcttcg ggcgaggttg ccagcgacga cgacagtccg
      721 ttgaccaagt cggtctgacg gtcgacgaag tcttcggcgg cttgcgaaac gcacgactcg
      781 atacggcgga tcccgctgcc gatcgaggat tccgagagga tcacgaacag gcccagctcg
      841 ccggtcgaat gcgagtgcgt tccaccgcaa aattcgaccg acggtccggc ggtaacgacg
      901 cgaatcgatt ccccgtagtt ctcgccggcc atccaaatcg cgccggtccg tttggcttgc
      961 tcgagcggga gctcgcgcgt cacgagatgc gaatcgtcgc gaatcatttc attgacgcgc
     1021 cgggtcacgg cccgcttctg ctcgccggtg agcgctccgc ccggccagcg gaagtcgaac
     1081 cgcattcgat cgatcccgac ccacgatccg gcttgattca cttcctcccc gaggacgtct
     1141 ttgagcgcgc gctgcaggag atgcgccgac gtatgatggc gacgaatctc ccggcgccac
     1201 cagtcgtaca ccgaagtccg cacgcgttcg ccggccgcga ttcgtccctc gcggacgatc
     1261 ccgtggtggg cgatcgcttc gccgagatac tgcgtgtcgc gcacttcgaa ggtcccgtct
     1321 ccggcggtaa ggatgccgcg atcgccgatc tgtccgccgc gttcggcgta gaacgacgac
     1381 gaatcgagaa
//
LOCUS       DASUCJ010000730         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_22976, whole genome shotgun sequence.
ACCESSION   DASUCJ010000730 DASUCJ010000000
VERSION     DASUCJ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22976"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFU90_08755"
     CDS             <1..1176
                     /locus_tag="VFU90_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5479907.1"
                     /translation="HLFEHMLFQGSEDVQANEHFELIQRAGGTLNGSTWLDRTNYYET
                     VPSNELALALWLEADRMGALLPAMTQQKLDTQRDVVKNERRWSMDNQPYGTWSERLPA
                     LTYPPTHPFHHSLIGSMEDLSAASLEDIAQFFSTFYRPDNAVLSIAGDFEPAQARALV
                     EQHFGAIPRGASPVPPLADMTLPPRFGATHREVVPDTVMVPRLFLAFRAPVFGSDEYY
                     AGSVLGAILGLGKSSRLQRVLVRQSEIASEATAFTFDLSQGADLLVIDATARPEVSSD
                     ALEAGVTQVVDAVRRDGVTGAEVERAIALIETAFVTSLQSAGERADKLSMFAAMFDDP
                     RLLNEQTDRYRAVTVERVNAFARERLGTDNRAVLLYVPKHGEEGEPDEAEAASGAAR"
BASE COUNT          209 a          402 c          439 g          197 t
ORIGIN      
        1 cacctgttcg aacacatgtt gttccagggc tcggaggacg tccaggccaa cgagcacttc
       61 gagctcatcc agcgcgcggg tggcacgctc aacgggtcca cgtggctgga tcgaacgaac
      121 tactacgaaa ccgttccgtc aaacgagcta gcgctcgcac tgtggttgga ggcagaccgc
      181 atgggcgcgt tgctgcccgc aatgacgcag cagaagctcg acacgcagcg cgacgtggtg
      241 aagaacgagc ggcgctggag catggacaac cagccgtacg gaacgtggtc cgaacgcctt
      301 cccgctctca cctatccgcc gacgcatccg ttccatcact cgctcatcgg ctcgatggaa
      361 gatttgtcgg cggcaagcct cgaggacatc gcgcaattct tctcgacgtt ctatcggccc
      421 gacaacgcgg tgttgtccat cgccggcgat ttcgagccgg cccaggcgcg tgcgctggtc
      481 gagcagcact tcggagcgat cccgcggggg gcgtcgcccg ttccgccgct cgcggacatg
      541 acgctcccgc cccgcttcgg tgcgacgcat cgcgaggtgg tgccggatac ggtgatggtg
      601 ccgcggttgt tccttgcatt ccgggcgccg gtcttcggga gcgatgagta ctacgccggc
      661 agcgtgttag gcgcgatcct gggactcggc aagtcgagcc ggttgcaacg cgtgctggtg
      721 cggcagagcg aaatcgcgag cgaggcgacg gcgttcacgt tcgatctgtc gcagggcgcc
      781 gatctgttgg tgatcgacgc gacggcgcga cccgaagtgt cgagcgacgc gctcgaggcc
      841 ggggtcacgc aggtggtcga cgcggtgcgt cgcgacggcg tgacgggtgc cgaggtcgag
      901 cgcgcgatcg cgctgatcga gacggcgttc gtgacatcgc tgcaatcggc gggcgaacgg
      961 gccgacaaac tctcgatgtt cgcggcgatg ttcgacgacc cgcgcctcct caacgagcag
     1021 acggatcgct atcgggccgt gaccgtggag cgcgtgaacg cgttcgcccg cgagcggtta
     1081 ggcacggaca accgcgcggt cctgctctac gtgcccaaac acggcgagga aggcgagccc
     1141 gacgaagccg aggcggccag cggagcggcg cggtgaagca cgcgtcgatt gcgttaggcg
     1201 gagcgcccgc ggcccggccg cgtcccgtcg cggcggcgcc caagccc
//
LOCUS       DASUCJ010000731         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_909475, whole genome shotgun sequence.
ACCESSION   DASUCJ010000731 DASUCJ010000000
VERSION     DASUCJ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909475"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..163
                     /locus_tag="VFU90_08760"
     CDS             14..163
                     /locus_tag="VFU90_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5479908.1"
                     /translation="MDRGIIITGGGALLRGLDKLLSEVTGVPAIVADDPLSCVALGTG
                     SRVHV"
     gene            160..837
                     /locus_tag="VFU90_08765"
     CDS             160..837
                     /locus_tag="VFU90_08765"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5479909.1"
                     /translation="MSGGMGIGAAIDARITAEADVAEEVARTVFSGGTVIFPNDTSYG
                     IGCDPYRSEAIDRIYAAKGRPDTRPLTIHVATPQEFLEYAGDNPLAVLAAKRLLPGPV
                     ILLIRKPAFVSDELAAGLQTLAFRVPDDPFARMLLERCGPLAGTTVNAGGRPRYRGDG
                     DRSMLPPADLLVEHGPTRYDRESSIVDLSGLHARLLREGAVPASRLAELLGPIERPTI
                     KVRTQPT"
     gene            834..>1135
                     /locus_tag="VFU90_08770"
     CDS             834..>1135
                     /locus_tag="VFU90_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479910.1"
                     /translation="MIRSTWALAAALALATAFAQAAPTVLPPAKPPVHLPPPPPVPSP
                     AAGGGGARFDLGVWTVHASAVDANFKTGDFSTPAKVVMTREGGDVSADRANGNYKT"
BASE COUNT          173 a          405 c          367 g          190 t
ORIGIN      
        1 ggccgccgac atcatcgatc gcggcatcat catcaccggc ggcggcgcgc tgctgcgcgg
       61 gctcgacaag ctcctgtccg aggtaacggg cgtgccggcg atcgttgccg acgacccgct
      121 ttcgtgcgtc gcgctcggca ccgggtcacg cgttcacgtg tgagcggcgg tatggggatc
      181 ggcgccgcga tcgacgcgcg cataacggcg gaagccgacg tcgccgaaga agttgcgcgg
      241 acggttttca gcggcgggac ggtcatcttt cccaacgata cgagctacgg catcgggtgc
      301 gatccctatc gctcggaagc aatcgaccgc atctacgcgg caaagggccg tcccgacacg
      361 cgtccgctga cgatccacgt tgcgactcct caggagtttc tagagtacgc gggcgacaat
      421 ccattggccg tgctcgccgc caaacggctc cttccggggc ccgtcattct cctgattcgc
      481 aagccggcgt tcgtgagcga cgaactcgcc gcgggactgc agacgctcgc gtttcgcgtg
      541 cccgacgatc cgttcgcccg gatgctgctg gagcggtgcg gtccgttggc gggaaccacc
      601 gtgaacgccg gcggccggcc gcgctatcgc ggcgacggcg atcgctcgat gctgcccccc
      661 gcggacctgc tggtcgagca cggaccgact cgttacgatc gcgaatcgtc gatcgtcgat
      721 ctgtcgggtc tccacgcacg cttgctgcgc gaaggtgccg ttccggcgag ccgcctggcg
      781 gaacttctcg gcccgatcga acgtcctacg ataaaggtac gtacgcaacc gacatgattc
      841 gctcgacctg ggcactcgcc gccgcgctcg ctttggccac cgccttcgcg caagccgcgc
      901 cgaccgtgct cccgccggcc aagccgcccg tccaccttcc gcccccgccg ccggttcctt
      961 ccccggccgc cggcggcggc ggcgcgcgtt tcgacttggg cgtgtggacg gttcacgcca
     1021 gcgccgtcga cgcgaacttc aagaccggcg acttctctac gcccgccaaa gtcgtcatga
     1081 cgcgcgaagg cggcgacgtt agcgccgatc gtgcgaacgg taactataag acgca
//
LOCUS       DASUCJ010000732         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_680212, whole genome shotgun sequence.
ACCESSION   DASUCJ010000732 DASUCJ010000000
VERSION     DASUCJ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680212"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..783)
                     /locus_tag="VFU90_08775"
     CDS             complement(61..783)
                     /locus_tag="VFU90_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEU5479911.1"
                     /translation="MNPARAMPRILAALTCAAMLAAMLAGCATAPNRDDPFEPMNRES
                     YKVHQVVDGHFIKPIAQAYVNYTPKPVQTVIANFFGNVDDVISVINDFLQGKLDKAGN
                     DMGRVILNTGFGLAGMIDIASEAGIPKGNEDFGQTLGVWGFPQGPYLFIPVWGPTTVR
                     DGSGSLIRLYAGPVGYIPDVPLRNTLYGIGALNARAQALSAESVALSAALDPYAFIRR
                     AYLQHRLYLLYDGKPPPEKDSQ"
     gene            complement(780..1349)
                     /gene="mlaD"
                     /locus_tag="VFU90_08780"
     CDS             complement(780..1349)
                     /gene="mlaD"
                     /locus_tag="VFU90_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEU5479912.1"
                     /translation="MNRATLDLWVGVFVTLGIAAILFLSLKVGNLLTVSHDPGYRLTA
                     EFDNIGGLKLRAPVKAAGVVVGRVEAIQLDPKTYQAVVTMRIDRGYAFTKDTIASILT
                     SGLLGEVYIGLDSGGDTKMLVNGDRIAHTQSAIVLEKLISQFMFDRAATAGGAGASNS
                     AGANGGKAPSAPAPAAGGAPSASGAGAIK"
     gene            complement(1399..>1584)
                     /locus_tag="VFU90_08785"
     CDS             complement(1399..>1584)
                     /locus_tag="VFU90_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479913.1"
                     /translation="IIKSVVFGIAVSLIAVFEGYNARPTAEGVSTATTRTVVASSLSI
                     LALDFVLTVFMFRGMGE"
BASE COUNT          268 a          531 c          519 g          266 t
ORIGIN      
        1 acgcgagcgc gcgccatgcg cggaccgggg ccgcgccgcg cagtggaaat ttgcaagtgt
       61 tcattgactg tccttttccg gcggcggctt gccgtcgtag agcagataga gacggtgctg
      121 caggtaggcg cgccggatga acgcatacgg gtcgagggcc gcggagagcg ccaccgattc
      181 ggcggaaagc gcctgcgcac gcgcgttgag cgcgccgatg ccgtacagcg tgttgcgcag
      241 cggaacgtcg ggaatgtatc cgaccggacc cgcatagagc cggatgagac tcccgctgcc
      301 gtcgcggacc gtcgtcggcc cccacaccgg gatgaagagg tatggcccct gcgggaagcc
      361 ccagacaccg agcgtctggc cgaaatcctc gttgcccttc gggatgcccg cctcggaggc
      421 gatgtcgatc atcccggcga ggccgaagcc ggtgttgaga atcacccgcc ccatgtcgtt
      481 gccggccttg tcgagcttgc cttgcaggaa gtcgttgatg accgagatca cgtcgtcgac
      541 gttgccgaag aagttcgcga tgacggtctg caccggtttc ggcgtgtaat tgacgtaggc
      601 ctgcgcgatc ggcttgatga aatgaccgtc gacgacctgg tgcaccttgt acgactcccg
      661 gttcatcggc tcgaacgggt cgtcgcggtt cggcgccgtc gcacacccgg ccaacatcgc
      721 ggcgagcatc gcggcgcagg tgagtgcggc aagaatgcgc ggcatcgcgc gagccggatt
      781 cacttgatcg cccccgcgcc ggacgcgctc ggcgctccgc ctgcggccgg ggcgggtgcg
      841 ctcggcgctt tgcctccgtt cgcgccggcg gaattcgacg cgccagctcc gccggcggtc
      901 gccgcgcggt cgaacatgaa ctggctgatc agcttctcga gcacgatcgc cgattgcgta
      961 tgcgcgatcc ggtcaccgtt gacgagcatc ttcgtatccc ctcccgaatc gaggccgatg
     1021 tacacctcgc cgagcagccc cgaggtgagg atcgacgcga tcgtgtcctt cgtgaacgcg
     1081 tagccgcggt cgatgcgcat cgtcaccacc gcctgatagg tcttcgggtc gagctggatc
     1141 gcctcgaccc ggccgacgac gacgcccgcc gccttgaccg gcgcgcgcag cttcagcccg
     1201 ccgatgttgt cgaattcggc cgtcagccga taaccggggt cgtggctcac cgtcagcaga
     1261 ttgccgacct tgagcgacag gaagaggatc gccgcgatgc cgagcgtgac gaacacgccg
     1321 acccagagat cgagcgtcgc gcggttcatc gggctcccgc ctccgcattg ctgccggcac
     1381 tgccgaatcg accgccgctc attcgcccat cccccggaac atgaataccg tcaacacgaa
     1441 atcgagcgcg aggatcgaga gcgagctcgc caccacggtg cgcgtcgttg ccgtcgacac
     1501 gccctcggcg gtcggacgcg cgttgtatcc ctcgaacacg gcaatcaggc tcacggcgat
     1561 tccaaaaacg acgctcttga tgat
//
LOCUS       DASUCJ010000733         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_160595, whole genome shotgun sequence.
ACCESSION   DASUCJ010000733 DASUCJ010000000
VERSION     DASUCJ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160595"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFU90_08790"
     CDS             complement(<1..448)
                     /locus_tag="VFU90_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479914.1"
                     /translation="MPVRFGLAVYRTLDRVAPAPYVETTLAEHALSSNDPAAALRYAL
                     RLPASPSRDELLGRIWRARGDDALAYEYFLAAPDVDAVQAAIERRASADPAAAYALER
                     ILAVRLARLQTHPDAVAEARWQMGLLANRRAWQELPGSPAQAAWLDR"
     gene            complement(577..>1147)
                     /locus_tag="VFU90_08795"
     CDS             complement(577..>1147)
                     /locus_tag="VFU90_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479915.1"
                     /translation="GVEPSTMRVGPVQMSTRMEGAAMDAPQLSLKDSGYGAGATFDVR
                     AGARNLNVDLSSNYEHLSRADSAAPAAALGTASSWQLPDSDTPLVIPNYADMSKLSIG
                     AAVAVPVVNGLTLNLNYAADRLIGGYGLPGLTNLDATDNSYGGKLTFEMPHSASTLSI
                     SARALRYQDNILPANALQTREDVNFTVKF"
BASE COUNT          198 a          377 c          397 g          175 t
ORIGIN      
        1 aacgatccag ccacgcagct tgcgccgggc tgcccggcaa ctcttgccac gcccggcgat
       61 tggcgagcaa gcccatttgc cagcgggcct cggcaaccgc gtcggggtgc gtctgaagcc
      121 gcgcgagccg tacggccaag atgcgctcca acgcgtaggc cgcggccgga tcggccgatg
      181 cacgccgttc gatcgccgcc tggaccgcgt ctacgtcggg agcggccaga aagtattcgt
      241 acgcgagcgc gtcgtcgccg cgcgcgcgcc aaatgcggcc gagcagttcg tcgcgcgacg
      301 gcgatgcggg caaccgcaga gcgtaacgaa gggccgccgc cggatcgttg ctcgacagcg
      361 cgtgctcggc gagcgtcgtc tcgacgtacg gcgctggagc gacacgatcg agggttcggt
      421 acacggccaa tccgaaacgc accggcacgc gcgtcggcag cgtgcccggc gcggcggcgg
      481 cgccgtcgaa cgcatacgag gcgagctgaa tcgtaccgaa tgcggccgac aggacgacga
      541 cggcggcaac ggcgccggca atcgcgaatc tagatcttag aattttactg tgaagttgac
      601 gtcttcgcgc gtctgcagcg cgttggccgg cagaatgttg tcctgatagc gcaacgctcg
      661 cgcggaaatc gacagcgtgc tcgccgaatg cggcatttcg aacgtcagtt tgccgccgta
      721 cgagttgtcg gtagcgtcga gattggtcaa acccggcagg ccgtagccgc cgatgaggcg
      781 gtcggccgcg tagttcagat tcagcgtaag accgttcacg accggaacgg cgacggccgc
      841 gccgatcgaa agcttgctca tatcggcgta gttcggaatc accagcgggg tgtcgctgtc
      901 gggcagctgc cacgacgaag cggtgccgag cgccgccgcc ggcgccgcgg agtcggcgcg
      961 cgagaggtgc tcgtagttgc tcgacaagtc gacgttgagg tttcgcgcgc ccgcccgcac
     1021 gtcaaaggtg gcgccggcgc cgtatccgct atccttgagc gacagctggg gcgcgtccat
     1081 cgccgcacct tccatccgag tgctcatctg caccggaccg acgcgcatcg tcgaaggctc
     1141 gacgccg
//
LOCUS       DASUCJ010000734         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1291542, whole genome shotgun sequence.
ACCESSION   DASUCJ010000734 DASUCJ010000000
VERSION     DASUCJ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291542"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFU90_08800"
     CDS             complement(<1..308)
                     /locus_tag="VFU90_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479916.1"
                     /translation="MDCYFHSNVPSVAPCVDCAKPICTTCRDENGTCPSCRLAAKVDA
                     AGGTRRQLGGRVPPRPQPQAQPQPAVVRKAEVAVPADPLESRALVALGYPLWPLALLS
                     L"
     gene            442..2196
                     /locus_tag="VFU90_08805"
     CDS             442..2196
                     /locus_tag="VFU90_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5479917.1"
                     /translation="MSRRSILRRLAREARPYYPRILAATLLGALAGVLTVVPPAAFRI
                     IINQVLAPPAGRLADLHALYFALLITAGALVVANLAIYFQTYLTAWSGQHLIARLRIR
                     LFERLLNLPLETFDRWRPGELIARFSTDLQLMTDAVSVSVPQLVVALVTFVSSFSMMI
                     YLDWLLTLSLVIVAPLVSYAVSKFQALISGSTHRAQARIADLSANLTEVLQGQRVVKA
                     FGREEYEVGRFATRNEDFFGAYMKLTQFIQTQPVVVSTIMVAAVVFIMWLSVREVLRG
                     RLDTGTVFMYWALLVNLMNPMNRVAAFFGDISKAIVGAGRVFEVLDLGVETRDEAGAR
                     PLPAVRGRIEFDDVSFTYGVGEQPALSHVDATIEPGEIVALVGPSGAGKTTMVNLVPR
                     FYSPQGGKVAIDGIDIATVRLRDLRRAIAIVPQDPQLFRGSVLDNIRYGRLEATDAEV
                     RASARDANCDDFVESFADGYATQVGERGVRLSGGERQRVAIARAILRDPRILILDEAT
                     SALDSHSEAMIEQALDRLLQGRTTLIIAHRLSTVRRANKVLYIEAGRLVEVGTHEQLL
                     ARGGAYARLHAAQFARSQ"
BASE COUNT          377 a          778 c          831 g          431 t
ORIGIN      
        1 agcgacagca gcgccagcgg ccagagcgga tagccgagcg cgaccagcgc ccgcgactcg
       61 agcggatcgg cggggacggc gacttcggcc ttccggacga cggccggctg aggctgggct
      121 tgaggctgag gccgcggagg aacgcggccg ccgagctgcc tgcgcgtccc gccggcagcg
      181 tcgactttgg cggccagacg gcaactcggg caggtgccgt tctcgtcgcg gcacgtcgta
      241 caaatcggtt tggcgcagtc gacgcacggc gcgactgacg ggacgttcga gtgaaaatag
      301 cagtccatgg tggcggcggt gtctcccctt ggcgtcgcgc ccggacctct gcgtgacggg
      361 cgccgttcgc gttctcttct cagctaccgg ctgcgaagtc aggaggtttc acacgcgccc
      421 ggcggcaaga gcgtgccgcc gatgagccgc cgctcgattc ttcgacgtct ggcgcgcgag
      481 gcgcgcccct actacccgcg aattctagct gcgacgctgc taggcgcctt ggcgggggtc
      541 ctcaccgtcg tgcctccggc ggctttccgg atcatcatca accaagtgct ggcgccgccg
      601 gcggggcgcc tggccgacct gcacgcgctg tatttcgccc tgctgatcac ggccggggcg
      661 ctggtcgtgg ccaatctcgc gatctacttt cagacgtatc tcaccgcctg gagcgggcag
      721 catctgatcg cgcgcttacg gatccggctg ttcgaacggc ttctcaatct gcccctcgag
      781 acgttcgatc gctggcggcc cggcgagttg atagcgcggt tcagcaccga cctgcagctg
      841 atgaccgacg cggtcagcgt ctcggtgccg cagctggtgg tcgcgctggt gacgttcgtt
      901 tcgtcgtttt cgatgatgat ctatctcgac tggctgctga cgctttcgct ggtgatcgta
      961 gcgccgctcg tgagttacgc ggtttcgaag tttcaagcgc tgatcagcgg cagcacccac
     1021 cgcgcgcagg cgcgcatcgc cgatctctcc gcaaacctta ccgaagtgct gcagggtcag
     1081 cgcgtcgtca aggcgttcgg acgcgaagag tacgaggtcg ggcggtttgc gacgcgcaac
     1141 gaagatttct tcggcgcgta catgaagctg acgcagttca tccagacgca gccggtggtc
     1201 gtttcgacga tcatggtcgc cgccgtcgtc ttcatcatgt ggctctcggt tcgggaagtg
     1261 ctgcgcggac ggctggacac cggtaccgtc ttcatgtact gggcgctgct ggtgaacctc
     1321 atgaacccga tgaaccgggt ggcggcgttc ttcggcgaca tcagcaaggc gatcgtgggc
     1381 gcggggcgcg ttttcgaggt gctcgatctc ggcgtggaaa cgcgcgacga ggccggcgcg
     1441 cgtccgctgc cggccgtgcg cggccgcatc gagttcgacg acgtctcgtt tacctacggc
     1501 gtgggagaac agccggcgct ctcgcacgtc gacgcgacga tcgaaccggg cgagatcgtg
     1561 gcgctggtcg ggccgtcggg cgccggcaag acgacgatgg tgaatctcgt gccgcggttt
     1621 tactcgccgc aaggcgggaa agtcgcgatc gacggcatcg acatcgcgac cgtacgcctg
     1681 cgcgacctcc gccgcgcgat cgcgatcgtt ccgcaggatc cgcagctgtt tcgcggatcg
     1741 gtgctcgata atatccggta cgggcggctc gaagcgaccg atgcggaagt gcgggcatcc
     1801 gcgcgcgacg cgaactgcga cgatttcgtg gagtcgtttg ccgacggata cgcgacccaa
     1861 gtcggcgagc gcggcgtccg cctctcggga ggcgaacgcc agcgcgtcgc gatcgcccgc
     1921 gcgatcctgc gcgatccgcg catcctgatc ctcgacgagg cgaccagcgc gctggacagc
     1981 cactcggagg cgatgatcga acaggcgctg gaccggctct tacaggggcg tacgacgctg
     2041 attatcgccc atcgcctgtc gacggtacgc cgtgccaaca aggtgctgta catcgaggcc
     2101 ggacgactgg tcgaggtcgg cacccacgaa cagctgctgg ctcgcggcgg cgcctatgcg
     2161 cgcctgcacg ccgcccagtt cgctcgttcg cagtaacgct ccgggacttt gagcggtagc
     2221 gaagcgcgac ggtggggcgc ggatgcggta acgccccggt tccgggacgc tcgctccaaa
     2281 cgtccatgtg tctccgctcg ctcgactcga ttttcgggag aacgaagttt tgaagaaacc
     2341 atccaggttt gcccgaaaat cgagacgccc gtcaccgggg cgttaccgcg aacgagctcg
     2401 gcgaagcgcg acgggtg
//
LOCUS       DASUCJ010000735         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_970752, whole genome shotgun sequence.
ACCESSION   DASUCJ010000735 DASUCJ010000000
VERSION     DASUCJ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970752"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFU90_08810"
     CDS             complement(<1..402)
                     /locus_tag="VFU90_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479918.1"
                     /translation="MASVAPDLRSASLLDWYARNRRRSADIFALVADSAFYARPIALR
                     HPFVFYEGHIPAFSFLTVNERALGESPVDPEFERLFERGIDPGSGDEAARLSRSDWPT
                     RDAVHAFGRACDERVQRALAHALDDPAVPRLV"
     gene            complement(414..>1228)
                     /gene="hppD"
                     /locus_tag="VFU90_08815"
     CDS             complement(414..>1228)
                     /gene="hppD"
                     /locus_tag="VFU90_08815"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU5479919.1"
                     /translation="AAFETAIKGGAQPVAPPSVLEDSHGRVVQAKIATYGDTVHSFVQ
                     RDGYRGIFMPGFVEQRKTLAAAEKPGLLFIDHCVGNVGWGEMDAWGDFYARVFGFSQL
                     VSFDDKDISTEYTALRSKVMTDARHRVKFPINEPAHGKKKSQIEEYLDFYRSAGVQHM
                     AIRTDDIAATIRALRANGVEFLDTPDSYYEMLEERVGRIDEAIEMLREQRILVDRDDL
                     GYMLQIFTKPLQDRPTVFFEIIQRKGSLSFGKGNFKALFVSIEREQEKRGTL"
BASE COUNT          225 a          387 c          396 g          220 t
ORIGIN      
        1 cacgagtcgc ggaactgccg gatcgtccag cgcgtgcgcg agcgcgcgtt ggacgcgttc
       61 gtcgcaggca cggccgaagg cgtgcaccgc atcccgcgta ggccaatcgg agcgagaaag
      121 ccgcgcggct tcgtcgcccg atccgggatc gatgccgcgt tcgaaaaggc gttcgaactc
      181 cggatcgacc gggctttcgc ccagcgcccg ctcgttgacg gtgaggaagc taaacgccgg
      241 aatgtggcct tcgtagaaca cgaacgggtg gcgcagcgcg atcggacgcg catagaacgc
      301 ggagtccgcc acgagtgcga aaatgtcggc cgagcgccgg cggttgcgcg cgtaccagtc
      361 caagagcgaa gcgctccgaa gatcgggggc taccgacgcc atggaaaacc tccttacagc
      421 gtcccgcgct tttcctgttc ccgttcgatc gagacgaaga gcgctttgaa attgcccttc
      481 ccgaacgaca ggctgccctt gcgctggatg atctcgaaaa acacggtcgg ccggtcctgc
      541 agcggcttgg tgaagatctg cagcatgtac cccaggtcgt cgcggtcgac caggatgcgc
      601 tgttcgcgca gcatctcgat cgcctcgtcg atccggccga cccgctcctc gagcatctcg
      661 taataggagt cgggcgtatc caggaactcc accccgttgg cgcgcagcgc gcgaatcgtc
      721 gccgcgatgt cgtcggtgcg aatcgccata tgctgaacgc cggcgctgcg gtagaagtcg
      781 aggtactctt cgatttgcga tttcttcttg ccgtgcgcgg gctcgttgat cgggaacttg
      841 acgcggtgtc gcgcgtcggt catcactttg gagcgcagcg ccgtgtattc ggtggaaata
      901 tccttgtcgt cgaacgaaac gagctgcgag aagccgaaga cgcgcgcgta gaagtcgccc
      961 caagcatcca tctcgcccca gcccacgttc ccgacgcagt gatcgatgaa aagcagtccc
     1021 ggtttttcgg cggcggcgag tgtcttacgc tgttcgacga accccggcat gaagatgccg
     1081 cgataaccgt cgcgctgcac gaacgagtga accgtatcgc cgtacgtcgc aatcttggcc
     1141 tgcacgacgc gcccgtgaga gtcctcgagt acggacggcg gcgcaaccgg ctgggcgccg
     1201 cccttgatcg cggtttcgaa tgcggcgg
//
LOCUS       DASUCJ010000736         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1108325, whole genome shotgun sequence.
ACCESSION   DASUCJ010000736 DASUCJ010000000
VERSION     DASUCJ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108325"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1080
                     /locus_tag="VFU90_08820"
     CDS             <1..>1080
                     /locus_tag="VFU90_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5479920.1"
                     /translation="PNNAALVVAGDVSHAAVFAKAQRYFGAIPHKTLPHAIARKPVAA
                     TGKTVEAQFPFPFEVLDLAYSIPGDTEPGEPAVSTLATLLENQRSPFYQALVETNIAL
                     AIEANADTQLRGGLLNVFIILNPGHRGDEAQAIFQSTMNRVLQDGFSPEIVTAAKRLT
                     IAERMYSADSIDGIGDLAGYTYGIVGEKIQNEDDRLAALTGADLLHAARTYLSRPNVV
                     GHLTPNDSPPRGSSNKSDAAASDDFSKRVPSGPIVEPAWIAKAVRTPTTARSALAPAA
                     FTLPNGLRVIVQQKPDRPTFVLRGQIATSPAFQPAGLEGIERLASAVADYGSAQYPFA
                     ARRKATDEMGAFVETGQSFSAQGESR"
BASE COUNT          202 a          384 c          324 g          170 t
ORIGIN      
        1 tcccaacaac gccgcgctcg tcgttgccgg cgacgtttcg cacgccgcgg tcttcgccaa
       61 ggcgcaacgc tatttcgggg cgattccgca taagacgctg ccgcacgcga tcgcgcgcaa
      121 accggtcgcc gcgaccggca aaaccgtcga agcccagttt ccgttcccgt tcgaggtgct
      181 cgatctcgcc tattccatcc cgggcgacac cgagcccggc gagccggcgg tcagcacgct
      241 cgcaacgctt ctcgagaacc agcgttcgcc gttctatcaa gcgttagtcg aaacgaacat
      301 cgcgctggcg atcgaggcca acgccgacac gcagctgcgc ggcggcctgc tcaacgtttt
      361 catcattctc aacccgggac atcgcggcga cgaagcacaa gcaatttttc aatcgacgat
      421 gaaccgcgtg ctgcaagacg ggttcagccc ggaaatcgtc accgccgcta aacggctgac
      481 gattgccgag cgcatgtact cggccgattc gatcgacggc atcggcgatt tagccggcta
      541 cacctacgga atcgtcggcg agaagattca aaacgaagac gatcgcctgg ccgcgctgac
      601 cggcgccgat ctgctgcacg ccgcccgcac ctacttgagc cgtcccaacg tcgttggcca
      661 cctcacgccc aacgacagcc cgccgcgcgg cagctccaat aagagcgacg ccgccgccag
      721 cgacgatttc tccaagcgcg taccctcggg tccgatcgtc gagccggcct ggattgccaa
      781 ggcggtgcgc acgccgacca ccgcgcgcag cgcgctcgcg ccggctgcat tcacgctgcc
      841 caacgggttg cgcgtgatcg tgcagcaaaa accggaccgc ccgaccttcg tgctgcgcgg
      901 gcaaatcgcg acgtcgccgg ccttccagcc ggccggtctc gaaggtatcg aaaggctggc
      961 ctcggcggtg gccgattacg gcagcgcgca gtatccgttt gcggcgcgcc gcaaggcgac
     1021 cgacgaaatg ggcgcgttcg tcgagaccgg gcagtcgttt tccgcgcaag gcgaatcgcg
//
LOCUS       DASUCJ010000737         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_404640, whole genome shotgun sequence.
ACCESSION   DASUCJ010000737 DASUCJ010000000
VERSION     DASUCJ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404640"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFU90_08825"
     CDS             complement(<1..763)
                     /locus_tag="VFU90_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU5479921.1"
                     /translation="MTHLAAVPRAGRRLRRRIAGLGIRGRLALLTLAFGLPFLGYIGF
                     SAVEQMAQERELAKERNLAFARVVAARLDDYIGDVDQLLATLSHAVRTAPDATAQNDA
                     LLRNLRPNLPSHVSNLAIWDATGNNVGTLDDALRAHPFTVADRSYFRRALEGRALVIE
                     APLVSRSDGAIMAIFARPVLVDGRVVGVVAASTKLGQLENLLKPEGALPAGSVIALID
                     QSGTILARSLDPDRWVGQSVAARGEIMANVQKREGT"
     gene            complement(874..>1167)
                     /locus_tag="VFU90_08830"
     CDS             complement(874..>1167)
                     /locus_tag="VFU90_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479922.1"
                     /translation="EHGTIANAVNFPAIEMARESPYRMAIANANVPNMLGQISTAMAQ
                     AGLNIHNMLNKSRGETAFTLVDVDSPIEPGVVGSIAAIGGVLSVRYIPAEDAG"
BASE COUNT          192 a          379 c          396 g          200 t
ORIGIN      
        1 ccgtgccttc ccgcttctgg acgttcgcca tgatctcgcc gcgagccgcg acgctctgtc
       61 cgacccagcg gtccggatcg agtgagcgtg cgaggatcgt cccgctctgg tcgatgaggg
      121 cgatgacaga gccagcgggc agcgctcctt ccggtttgag cagattctcg agctgcccca
      181 gtttcgtcga cgcggccacg acaccgacga cccggccatc gacgaggacg ggtcgtgcga
      241 atatcgccat gatcgctccg tcgctgcgcg aaacgagcgg cgcctcgatg accagcgctc
      301 gtccttcaag cgcgcggcgg aaataggaac gatcggcgac ggtgaacgga tgtgctcgca
      361 aagcatcgtc cagcgtcccg acgttgtttc ccgtcgcgtc ccatatcgcg agattgctga
      421 cgtgggacgg cagatttggc cgcaggttgc gcagcagagc gtcgttttgc gccgtggcat
      481 ccggcgccgt gcgcacggca tgcgaaagtg tggccaacaa ctgatccacg tccccgatgt
      541 agtcgtcgag gcgcgcggcg acgacgcggg cgaacgcgag gtttctctcc ttggcgagtt
      601 ctcgctcctg ggccatctgt tccacggcgc tgaaaccgat gtagccgagg aacggcaagc
      661 cgaatgccag ggtgagcagc gccagccggc cccggatgcc gaggccggcg attcgccgcc
      721 gcaggcggcg tcctgcccgc ggtactgccg caaggtgggt caccagcgcc gtgtcgtgtc
      781 gtcggtgctc ggggcggcag cagaagccat gccacgcccg gggcggaacc agttcgaccg
      841 gcctgacgca ggcgtgcgcc aagcaacgtc gcgtcagccc gcgtcttcag cggggatgta
      901 gcggaccgac agcacgccgc cgatcgcggc gatcgacccg acgacgccgg gctcgatcgg
      961 actgtccacg tcgacgagcg tgaacgccgt ctcgccgcgc gatttgttga gcatgttgtg
     1021 gatgttcaac cccgcctggg ccattgcggt cgatatctgg ccgagcatat tgggaacatt
     1081 ggcgttggcg atcgccatgc gataaggcga ctcgcgggcc atctcgatcg ccgggaaatt
     1141 gaccgcattg gcaatggtgc cgtgctc
//
LOCUS       DASUCJ010000738         1248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1398680, whole genome shotgun sequence.
ACCESSION   DASUCJ010000738 DASUCJ010000000
VERSION     DASUCJ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398680"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..182)
                     /locus_tag="VFU90_08835"
     CDS             complement(12..182)
                     /locus_tag="VFU90_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479923.1"
                     /translation="MTLTTLILTNVVLAGALIYALVHFLTHAIHADRRHRASRVAELS
                     ALPERVRDRIAA"
     gene            314..976
                     /locus_tag="VFU90_08840"
     CDS             314..976
                     /locus_tag="VFU90_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479924.1"
                     /translation="MRAKLAILVAAVLAVAVAIGFYIGFLTGGPTPYRPPAASGAAGR
                     TVDLTLQTVAAIGPKLSPNETWVSYLVRENGVWHRSTVWTLPANTLVHVTVYNFDGKS
                     GLRNPFLAQARGVVGGSFAIDGKPTSTIAPDDASHTFAIPGMGVIVPIQGVDDNAPHQ
                     CDFAPCPMSNAHHTITFAFRTGKKGHYRWQCFVPCAAGFVYGFGGPMQTVGYMDGYVD
                     VV"
     gene            966..>1248
                     /locus_tag="VFU90_08845"
     CDS             966..>1248
                     /locus_tag="VFU90_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479925.1"
                     /translation="MWSSRSHGQRIAALTVIGTAIVLPLVVFVLGPHMPPGKGTPVAS
                     SQVTDNTVLLVVMTPFTVFILVYLGYALLAFRAAKDEGLRDGAPIRGHAR"
BASE COUNT          194 a          416 c          426 g          212 t
ORIGIN      
        1 gtacagaaga gctacgcggc gatgcggtcg cgcacgcgct ccggcagtgc ggagagctcg
       61 gcgacgcggc ttgcgcggtg acggcggtcg gcgtgaatcg cgtgcgtcag gaagtggacg
      121 agcgcgtaga tcaacgcacc cgcgagcacg acgttcgtga ggatcagtgt ggtgagtgtc
      181 atgcatccag ggttcccggc ctgcctgaaa ggtcgatgag agaaccctgt gtgtcggctg
      241 agagatgacg cccagatgac actttcgtca caaacctgtc gcggtggttc gtggggcgat
      301 atatcggcgg gcggtgcgcg cgaagcttgc gatcttggtc gccgcagtgc tggcggtcgc
      361 cgtcgcgatc ggcttctaca tcggcttcct cacgggtggg ccgacgccgt accggccacc
      421 ggctgcgagc ggggccgcgg gtcgcacggt cgacctgacg ctgcagaccg ttgccgccat
      481 aggcccgaag ctctccccga acgagacctg ggtgtcctat ctcgttcgcg agaacggcgt
      541 gtggcaccgc agcaccgtct ggacgctgcc cgcgaacacg ctcgtgcacg tgaccgtcta
      601 caacttcgac ggcaagagcg gtcttcgcaa cccgttcctc gcgcaggcgc gcggtgtcgt
      661 cggcgggtcg ttcgcgatcg acggcaagcc gacctcgacg atcgcgccgg acgacgcgtc
      721 gcacacgttc gcgatcccgg ggatgggcgt catcgtcccg atccagggcg tcgacgacaa
      781 cgccccccac cagtgcgact tcgcgccgtg cccgatgtcg aacgcgcacc acacgatcac
      841 gttcgcgttc cgcacgggga agaagggcca ctaccgctgg cagtgcttcg tgccgtgcgc
      901 ggccggtttc gtctacggct tcggcggccc gatgcagacg gtcggctaca tggacggata
      961 cgtcgatgtg gtctagccgg agtcacggcc agcgcatcgc tgcgctcacc gtgatcggaa
     1021 cggcgatcgt cctacccctc gtcgtcttcg tgctcggccc gcacatgccg ccgggcaagg
     1081 gcacaccggt cgcgtcgagc caggtgaccg acaacaccgt gctgctcgtc gtgatgacgc
     1141 cgttcaccgt cttcatcctc gtctacctcg gttacgcgct gctcgctttc cgcgccgcga
     1201 aggacgaagg gctacgggac ggcgcgccga tccggggtca cgcgcgcg
//
LOCUS       DASUCJ010000739         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_512284, whole genome shotgun sequence.
ACCESSION   DASUCJ010000739 DASUCJ010000000
VERSION     DASUCJ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512284"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFU90_08850"
     CDS             <1..261
                     /locus_tag="VFU90_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479926.1"
                     /translation="NFAIDGRAGELRFAAEARDVVSGRRLTVETTQPGLQLYTGKPGA
                     FALETQHFADSPNHPNFPSTVLRPGQTFRSTTAYAFSAEALD"
     gene            complement(253..2085)
                     /gene="typA"
                     /locus_tag="VFU90_08855"
     CDS             complement(253..2085)
                     /gene="typA"
                     /locus_tag="VFU90_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5479927.1"
                     /translation="MKTRADLRNVAIIAHVDHGKTTLVDAMLKQSGVFRQGQHVESRV
                     LDSNPLERERGITILSKNTAVQHGAVKFNIVDTPGHADFGGEVERVLQMVQGVLLLVD
                     AAEGVMPQTRFVLRKALELDLKAIVVVNKIDRRDARALEVVNETFDLFVELGANDEQA
                     DFPVIFTNAKAGVAKRALDDSSDSLEPLFETIVDRIPEPPAEEGSFQLLISAIDHNNY
                     VGRIGIGRIFRGRSRLNAPIVKVARDGAVAAGLRLTSLLTFAGLERIEVEEASAGDIV
                     CVSGIEDLNIGDTIADAAAPEGIHAVAVDEPTVSMSFSVNNSPFAGREGRYLTSRQIR
                     ERLMRELESNVALRVSATASPDTFEVRGRGELHLSILIETMRREGYELAVSKPQVIVT
                     ERDGKRWEPVEYVVVDVPEEYSGAVIESLGRRKAVMSNMLNVGDSRRLEYTMPTRAIF
                     GLRGELLTLTRGTAVMSHTYYDHQEWQGELPGRGVGALVASETGRTTGYALMGLEQRG
                     DYFVGPGVDVYEGMIVGRANDDKDIALNVVREKKLTNMRAAGSDENVKLAPPDELSLE
                     RAIEFIEDDELVEVTPKSIRLRKRMLSEGERLKTRKREAAARSV"
     gene            complement(2132..>2943)
                     /locus_tag="VFU90_08860"
     CDS             complement(2132..>2943)
                     /locus_tag="VFU90_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5479928.1"
                     /translation="GPIDGKVPSVDTLRQMQLFLNLVGLALATSRVHRAEVARRELLE
                     SNARLQNEFFGEVSHEVRSPLAAIAGAASLLEIHYETMDPKRRVELLNVLGNATTRLS
                     KIFEDFLLLSRMDADRLSLHVTRVLPASIIEESIARMRSEHAGRRFKVGYFDPTAGVM
                     ADEGRLVQVLANLLSNAVKYSNPDSPIYVDVKPAGDREIVVSVTNEGPGIPEGDRDKL
                     FKRFARLTQDNDSTGLGLYICRELVTKMGGSIGVESEPGDRTTFWFTLPRG"
BASE COUNT          474 a          966 c          960 g          543 t
ORIGIN      
        1 aacttcgcga tcgacggacg cgccggcgag ctgcgctttg ccgccgaagc ccgcgacgtc
       61 gtctccggcc ggcggttgac ggtcgagacg acgcaacccg ggttacagct ctacaccgga
      121 aaacccggcg cgttcgcgct cgaaacgcag catttcgccg actcgcccaa ccatccgaac
      181 tttccgagca ccgtcctgcg gccgggccag acgttccggt cgaccaccgc gtacgcgttc
      241 tcggccgagg cgttagactg atcgtgcggc ggcttcgcgt ttgcgcgtct tgagccgctc
      301 gccttcggag agcatgcgct tgcgaagccg gatcgacttg ggcgtaacct cgaccagttc
      361 gtcgtcctcg atgaactcga tcgcgcgttc cagcgacagc tcgtcgggcg gcgccagctt
      421 gacgttctcg tcggaaccgg cggcgcgcat gttggtgagc tttttttcgc gcacgacatt
      481 gagggcaatg tctttgtcgt cgttagcgcg gccgacgatc atcccttcgt agacgtcgac
      541 gccggggccg acgaagtagt cgccgcgctg ctcgagaccc atcagcgcgt agccggtggt
      601 ccgtccggtt tcgctcgcga cgagcgcgcc gacgccccgg ccgggcagct cgccctgcca
      661 ctcttgatga tcgtaatacg tgtgcgacat caccgccgtg ccgcgcgtga gcgtcagcag
      721 ctcgccgcga agcccgaaaa tcgcgcgcgt cggcatggtg tattcgaggc ggcgcgagtc
      781 gccgacgttg agcatgttcg acatcaccgc cttgcgccgg cccagcgact cgatcacggc
      841 tcccgaatat tcttcgggga cgtcgacgac gacgtactcg accggttccc agcgcttccc
      901 gtcgcgttcg gtaacgatga cctgcggctt ggaaacggcg agttcgtaac cctcgcgccg
      961 catcgtttcg atcaggatcg ataggtgcag ctcgccgcgt ccgcgcactt cgaacgtgtc
     1021 gggcgacgcg gtagcggaga cgcgcagcgc cacgttcgac tcgagctcgc gcatcagccg
     1081 ctcgcggatt tggcgcgagg tcaggtagcg tccctcgcgg ccggcgaacg gcgagttgtt
     1141 cacgctgaac gacatcgaga cggtcggctc gtcgaccgcg accgcgtgga tgccttccgg
     1201 cgccgccgcg tcggcgatcg tgtctccgat gttcagatcc tcgatccccg agacgcacac
     1261 gatgtcgccc gccgacgctt cctcgacctc gatccgctcg agccccgcaa acgtcagcag
     1321 cgaggtcagg cgcaatccgg cggcgacggc gccgtcgcgc gcgaccttga cgatcggtgc
     1381 gttcaaccgc gagcgtccgc ggaaaatgcg gccgattccg atccggccga cgtagttgtt
     1441 gtggtcgatc gcggagatga gcagctgaaa ggagccttcc tcggcgggcg gctcgggaat
     1501 gcggtcgacg atcgtctcga acagcggctc gagcgaatcg ctggaatcgt cgagcgcgcg
     1561 tttggccact cccgctttcg cgttggtgaa gatgacggga aagtcggctt gctcgtcgtt
     1621 cgcgccgagt tcgacgaaca ggtcgaaggt ttcgttgacc acttccagcg cgcgagcgtc
     1681 gcggcgatcg atcttattga cgacgacgat cgctttgaga tcgagctcca gcgctttgcg
     1741 caagacgaaa cgcgtttgcg gcatcacccc ttcggcggca tcgaccagca gcagcacgcc
     1801 ttgcaccatc tgcagcacgc gctcgacctc tcccccgaag tcggcatgcc cgggcgtgtc
     1861 gacgatgttg aatttcacgg ccccgtgctg caccgcggtg tttttggaga ggatcgtgat
     1921 cccgcgctcg cgttcgagcg ggttagagtc gagcacccgg ctttcgacgt gctggccttg
     1981 gcggaagacg ccgctctgtt tgagcatggc gtcgaccagc gtcgtcttcc cgtgatcgac
     2041 gtgggcgatg atggcgacgt tgcggaggtc ggcgcgggtc ttcacggccg gtctggttcg
     2101 cgcggcccta cgccgcggac ctggcgcggc ttcagccgcg cggcagcgtg aaccagaaag
     2161 tagtccggtc gcccggctcg ctctcgacgc cgatcgagcc gcccatcttg gtcaccagct
     2221 cgcggcaaat gtagagtccc aagccggtcg agtcgttgtc ctgggtcaag cgggcgaagc
     2281 gtttgaagag cttgtcgcgg tcgccttcgg ggatgcccgg gccctcgttg gtgaccgaaa
     2341 cgacgatctc ccggtcgccc gcgggtttga cgtcgacgta gatcgggctg tccggattcg
     2401 agtacttgac ggcgttggag agtaagttgg cgaggacttg cacgaggcgt ccttcgtcgg
     2461 ccatcacgcc ggcggtcggg tcgaaatacc ccaccttgaa ccgccggccg gcatgctcgc
     2521 tgcgcatccg cgcgatcgac tcttcgatga tggaggccgg caaaacgcgc gtgacgtgca
     2581 gggacaggcg gtccgcatcc atccgcgaca gcagcaaaaa gtcttcgaaa atcttcgaca
     2641 gccgggtcgt cgcgttgccg agaacgttga gcagctcgac ccggcgcttg gggtccatcg
     2701 tttcgtagtg aatttccagc agcgatgcgg cgccggcgat cgcggccagc ggcgagcgaa
     2761 cttcgtgcga aacctcgccg aagaactcgt tctgcagccg cgcattgctt tcgagcagct
     2821 cgcgccgcgc aacctcggca cgatgcacgc gcgaggtcgc gagcgccaat cccaccaggt
     2881 tgaggaacag ctgcatctgc cgcagcgtat cgaccgacgg aactttgccg tcgatcggcc
     2941 cgt
//
LOCUS       DASUCJ010000740         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_802813, whole genome shotgun sequence.
ACCESSION   DASUCJ010000740 DASUCJ010000000
VERSION     DASUCJ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802813"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFU90_08865"
     CDS             complement(<1..424)
                     /locus_tag="VFU90_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479929.1"
                     /translation="MPSASLLVWLFTRSLILPHEPATLVLSPRDFPSSGSVQGLVETA
                     VAPVTSDRFDAGGTGVGEPAKFGAYGGSWTQTTYRLGDLDITNPLRPGTPLVLPDTAG
                     LEGVSIMTAPSLDDVSATGPLFDLRPLRPGATPSFTFEG"
     gene            complement(493..>1796)
                     /locus_tag="VFU90_08870"
     CDS             complement(493..>1796)
                     /locus_tag="VFU90_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5479930.1"
                     /translation="SHVIVAGAGFAGLAAARALEARGASVTVIEARDRVGGRVLTIRD
                     GFANAQHAEGGADFIDSTQTSLISLARELKVKLVRINRRGFGYYGPDPSGRMSIQSIN
                     AVWESLGERLGPLIHAYKLADHRWTGPIASSIARQSVGSWLRSIKAPEWVVERLVGLR
                     GLFLADPDELSLLALVDFFADVDDAGWGETYRAADGNDLIATRIARDLASPIQFGTIL
                     RRLRQHASGIGATVESQSRLSEISADYAIVALPPTPTHAVQFEPGLPAAQRKALESLR
                     TGCATRLLVQFGRRFWDKRGRPTGFGSAQPTGAVWDGNEQQKGPAAILSFLAGGGASE
                     ELQALLRTGGVDGVVSTLQWLGRPAKVLASRTITWEDDPWAAGGYAFFDPGFDPALRD
                     WLALPHGRVFFAGEHTSVRWQGYMNGAIESGQRAAAEIAAM"
BASE COUNT          326 a          608 c          577 g          285 t
ORIGIN      
        1 ctccctcgaa tgtgaacgac ggtgtcgcgc ccgggcgaag cggccgcaga tcgaacagcg
       61 ggccagtcgc actcacatcg tccagacttg gcgccgtcat gatcgataca ccttcgagac
      121 ctgcggtgtc gggcaagacg agcggcgtgc cggggcgcaa tgggttggtg atatcgagat
      181 cacccagtcg ataggtcgtc tgcgtccagg atcccccgta cgcgccgaac ttcgcgggct
      241 cgccaacacc ggtgccgccg gcgtcgaagc gatcgctcgt gacgggcgcg accgcggtct
      301 cgacgagacc ttgcacgctt ccactcgagg ggaaatcgcg cggcgagaga acgagcgtcg
      361 caggctcgtg cggcaggatg agcgaacgtg tgaagagcca gaccagcagc gacgcagacg
      421 gcattcgcgt gaaaagtata ccgggaatac ttttcctgtc ctcttttgtg gaaagtattc
      481 ccggtatact tcttacattg cggcgatttc ggccgcggcg cgctggccac tctcgatcgc
      541 gccgttcatg tagccctgcc agcgcacgct cgtgtgctcg ccggcgaaga acacgcgacc
      601 gtgcggaagc gcgagccagt cgcggagcgc ggggtcgaag cccggatcga aaaacgcgta
      661 ccctccggcg gcccatggat cgtcttccca agtgatcgtg cgcgacgcca gcaccttcgc
      721 tggccggccg agccactgca gcgtcgagac gactccgtcc acaccgccag tgcgaagcaa
      781 cgcctgaagc tcttccgacg cgccgccgcc ggcgaggaag ctgaggatcg ccgccggtcc
      841 cttctgctgc tcgttgccgt cccagacggc tccggtcggt tgtgccgagc cgaaaccggt
      901 cggccggccg cgcttgtccc aaaaccgccg gccaaactgc acgagcagtc gggtggcaca
      961 accagtacgc aggctctcga gcgccttgcg ttgagccgcc ggcagcccgg gttcgaactg
     1021 caccgcgtgc gttggcgtcg gcggcagcgc gacaatcgcg tagtccgcgc tgatttcgct
     1081 gagccgcgat tgcgattcga cggtcgcacc gatcccagac gcatgctggc gaagacggcg
     1141 caagatcgtc ccgaactgaa tgggcgacgc gaggtcgcgc gcgatccggg tcgcgatcag
     1201 atcgttgccg tcggcggcgc gatatgtttc tccccacccg gcatcgtcga cgtccgcgaa
     1261 gaaatcgacg agcgcgagca gcgacagctc atccggatcg gcgaggaaca atccacgcag
     1321 gccgacgagc cgctcgacca cccattctgg cgctttgatc gatctgagcc acgagcccac
     1381 tgactgccgc gcgattgacg acgcgatcgg ccctgtccac cgatggtccg ccagcttgta
     1441 ggcgtggatg agcggcccaa gccgctcgcc gagcgactcc cacacggcgt taatcgactg
     1501 gatcgacatt cgcccgctcg gatcgggacc gtagtacccg aagccgcggc gattgatgcg
     1561 cacgagcttg accttgagct cacgcgcgag ggaaatcaat gacgtctgcg tggagtcgat
     1621 gaaatccgca ccgccctccg cgtgttgtgc gttggcgaac ccgtcgcgaa tcgtcaacac
     1681 gcggccgccg acgcgatcgc gcgcctcgat cacggtgacc gatgcaccgc gcgcttcgag
     1741 cgcgcgcgcg gcagcgagac ccgcaaatcc cgcgcccgcc acaatcacgt gtgatg
//
LOCUS       DASUCJ010000741         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_527533, whole genome shotgun sequence.
ACCESSION   DASUCJ010000741 DASUCJ010000000
VERSION     DASUCJ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527533"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFU90_08875"
     CDS             <1..992
                     /locus_tag="VFU90_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5479931.1"
                     /translation="ERKLLTIRTTTAYSGGAGTRHTLAPAELDAFETARRRYTGDWVR
                     AGIVKFFMDGVVETYTADLLTPYAGGVASARAYYAPTAFAAMLVELDRRGFAVMTHAI
                     GDGAVRATLDGYQAAIAANGPRDRRWRVEHIEVCDPHDVPRFAELGVIASMQPYHFCC
                     PAPDGSDTWDRHLGAARWPEGFVWQDIASAGTVLVHGSDWPVVTIDPMIGIYSALTRE
                     DPHGDPPGGWFPQQRLTLSQALAGYTRNAAYAAFMEDRIGTLEAGKLADLVVLDRDLR
                     AIPPAEILNVTIEKTVAGGRVVYEGGAVPDRVSELPVRAAPGCSCRRFAASRT"
     gene            complement(1095..1280)
                     /locus_tag="VFU90_08880"
     CDS             complement(1095..1280)
                     /locus_tag="VFU90_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479932.1"
                     /translation="MNFVEENYYAVAPLIRDWLVAQVLLALFIGLVAIVMAIVRTKEG
                     FKEERSRRSSRTGTFEP"
     gene            1622..1978
                     /locus_tag="VFU90_08885"
     CDS             1622..1978
                     /locus_tag="VFU90_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5479933.1"
                     /translation="MTNRGVPFVMYPVSDMARSVAFYRDVLGLKKEGLESDFWVEFDV
                     GGVTFGLGSFEQAGKPGTAQSFSIEVPDMASAREELSGKGHQSTEPFETQICFISMLR
                     DPDGNQIWLHQSKPAA"
     gene            1975..>2413
                     /locus_tag="VFU90_08890"
     CDS             1975..>2413
                     /locus_tag="VFU90_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEU5479934.1"
                     /translation="MRRALIAAAFVAAALSGSPAARAQQDPAYAQLDRLVGTWHSPGT
                     FVDSAYSKAGTADATTACAWSDGRFYLICQQTVVMNGKTTHGIAIYTYDLDNKKYRFY
                     NVSVTRANGTDIVVTPTSITYAGEFTDEGNHVLTRTLNLWENPQ"
BASE COUNT          423 a          799 c          799 g          392 t
ORIGIN      
        1 acgaacgcaa gctgctgacg atccgaacga cgaccgcgta ctccggcggc gcgggaacgc
       61 ggcatacgct cgcgccggcc gagctcgacg cgttcgaaac cgcgcgccgc cgttacaccg
      121 gcgactgggt gcgcgccggc atcgtaaagt tcttcatgga cggcgtcgtc gaaacgtaca
      181 cggccgacct gctgacgccc tacgccggcg gcgtcgcctc ggcgcgagcc tattacgcgc
      241 caaccgcatt tgccgcgatg ctcgtcgagc tcgaccggcg cggcttcgcg gtgatgacgc
      301 acgccatcgg cgacggcgcg gtgcgcgcga cgctcgacgg ctatcaggcg gcgatcgcgg
      361 ccaacggtcc gcgcgaccgg cgctggcgcg tcgagcatat cgaagtgtgc gatccgcacg
      421 acgttccgcg ctttgccgag ctcggcgtca tcgcgtcgat gcagccgtac catttctgct
      481 gcccggcgcc cgacggcagc gatacgtggg atcgccatct cggggcggcg cgctggcccg
      541 aaggcttcgt gtggcaggac atcgcgagcg ccggtaccgt gctcgtgcac ggcagcgatt
      601 ggccggtcgt gacgatcgat ccgatgatcg gcatctactc cgcgctgacg cgcgaagatc
      661 cgcacggcga tccgcccggc ggctggtttc cacagcagcg cctcacgttg agccaagccc
      721 tcgcggggta cacccgcaac gcggcctacg ctgcgttcat ggaagatcga atcggaacgc
      781 tggaagccgg aaagttggcg gacctcgtcg tgctcgatcg cgatctgcgc gcgattccgc
      841 cggcggaaat tctgaacgtc acgatcgaga aaaccgttgc cggcgggcgc gtcgtctacg
      901 agggcggcgc cgttcccgat cgcgtttcgg aattgccggt gcgcgcggcg cccggatgct
      961 cttgccggcg tttcgccgcg tcgcgcacgt gatgggcgta aaagaagttt acgatcgtga
     1021 ggctggcgtc gagctgacgg ctcgtccgcc cgatcggaaa actccgaaga tattgaacgc
     1081 cgccgggcgc cgaactacgg ctcgaacgta ccggtgcgtg acgagcgccg cgagcgctct
     1141 tctttgaatc cctctttggt gcggacgatc gccatcacga tcgccaccaa gccgatgaaa
     1201 agcgcgagca gcacctgcgc caccagccag tcgcgaatca acggagcgac ggcgtagtag
     1261 ttctcttcga cgaagttcat gcggacgaac cccggctcac gtcgctagta tagcaggaac
     1321 ggcgcctgcg gcgctccgct caggatgaca cggggcgctc cgctcaggat gacacagagc
     1381 gctccgctcg tccttcgact gcggcgctgc gcgcctccgc tcaggatgac acagagcgct
     1441 ccgctcgtcc ttcgactgcg cgccttcggc gctccgctca ggatgacaca gggcgctccg
     1501 ctcgtccttc gaccgcggcg ctgcgcgcct ccgctcagga tgacaggggg cgcttcgctc
     1561 gggatgacac gggtggggaa gggcgaagga ttggcgccta gccgaaagga gcgtattgct
     1621 aatgaccaac cgcggagtgc ccttcgtgat gtatccggtg agcgacatgg cgcgctcggt
     1681 cgcgttctat cgcgacgtgc tcgggctgaa gaaggagggg ttggagtcgg acttctgggt
     1741 cgagttcgac gtcggcggcg tgaccttcgg tttgggcagc ttcgagcagg ccggcaaacc
     1801 gggaacggct cagtcgtttt cgatcgaagt cccggacatg gcgtcggcgc gcgaagaact
     1861 cagcggcaaa ggccaccagt ccaccgagcc gttcgagacg cagatatgct tcatctcgat
     1921 gctgcgcgat cccgacggca atcagatctg gctgcatcaa tccaagccgg ccgcatgagg
     1981 cgcgcgctga ttgcggcggc gttcgtcgcc gcggcgcttt cgggttcgcc ggccgcgcga
     2041 gcgcagcagg acccggcgta cgcgcagctc gaccggctgg tcgggacctg gcattcgccg
     2101 gggacgttcg tcgacagcgc ctacagcaaa gccggaaccg cggacgcgac cacggcgtgc
     2161 gcgtggtcgg acggccggtt ttacttgatc tgccagcaga ccgtcgtcat gaacggcaaa
     2221 acgacgcacg gcatcgccat ctacacctac gatctcgaca acaagaagta ccgcttctac
     2281 aacgtcagcg ttacccgggc gaacggcacg gatatcgtgg tgacgcccac cagcatcacg
     2341 tacgccggcg aattcaccga cgagggcaac cacgtgctaa cgcgcacgct caacctctgg
     2401 gaaaacccgc agc
//
LOCUS       DASUCJ010000742         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_664919, whole genome shotgun sequence.
ACCESSION   DASUCJ010000742 DASUCJ010000000
VERSION     DASUCJ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664919"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..786)
                     /locus_tag="VFU90_08895"
     CDS             complement(163..786)
                     /locus_tag="VFU90_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479935.1"
                     /translation="MGEPSPSFEGVVGDWYVTEQAGARGLVVDGSQWRNGTPSANLAD
                     QAKRLYGDRYAEFLDGVKAFAFFPLAVWNGTPPVGDMRISVRFYPFAGKVDQGAGIAF
                     AISPEGTYLGIRANALEDNLLYFKVVKGRRTVFDDVRNVPTATKTWHTLSLELRGKHV
                     AATIDGQQRFEKALDSVPVGRVGLWSKADSQVLFDDFVVESLRPASK"
     gene            complement(1032..>1668)
                     /locus_tag="VFU90_08900"
     CDS             complement(1032..>1668)
                     /locus_tag="VFU90_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479936.1"
                     /translation="WFATAIASAGVLAAVGLRFDLSGVRRATVVVLVALVTGVTLFLV
                     AGSHRGYDWSEQRRASLPPAAALGLRRIRQPLRLDVFLDRDDSRRTQLETDVIAKLYL
                     ARPDLVVRTPLDDRTLVAEGQRDADYGRIEVHVGDVTRETRSTSRRELTTLIFEAANM
                     PAPDWSSPSYPGFPFVLEGTRRTLLALLAYLVLPLGLLGTGVLLTQRRVIR"
BASE COUNT          294 a          528 c          573 g          273 t
ORIGIN      
        1 gccgcgcgag caggcgcatc aagcggtcgc gcgctcgtcc ttgcaaccca aaacgcgaag
       61 cgccctcgct cgtggaagtg gtggaaccat cggcacgtcc ggttcgttcg gcgcgccgcg
      121 aggcccgccg ggcgccgaga cgctcgcaag atgggcgcag attcacttcg acgcggggcg
      181 gagcgactcg actacgaagt catcgaatag gacctgcgag tcggccttcg accacaaccc
      241 aacccgtccg acggggacgc tatcgagcgc tttttcgaat cgctgttgcc cgtcgatggt
      301 tgcggcgacg tgctttccgc gaagttcgag gctgagggtg tgccacgtct tcgtggccgt
      361 cgggacgttc cgcacgtcgt cgaatacggt gcgccgccct ttgaccactt tgaagtagag
      421 caaattgtct tcgagggcgt tggcgcggat gcccagatac gtcccctcgg gggagatggc
      481 gaacgcgatt cccgcgccct gatccacctt gccagcgaac gggtagaagc gaaccgagat
      541 acgcatatcg cctaccggcg gcgtcccgtt ccagaccgcg agcgggaaga acgcgaacgc
      601 cttcacgccg tcgagaaact cggcgtagcg atctccatag agacgtttgg cttggtccgc
      661 gaggttggcc gagggcgtgc cgtttcgcca ttggcttccg tcgacgacca atccgcgggc
      721 ccccgcctgt tccgtcacgt accaatcgcc gactacgcct tcgaacgacg gcgacggctc
      781 tcccatggtt tctgcgtcga acgtgacctt ggtgctcccc gtcgaggcgc tcgcgggtgc
      841 cgcgcctgcg ccgacaggct gcggcgactc cggaatcgga gaggagtcga tcgcctgtgt
      901 cgtcgccggg ggagtcgaag gggctgcagg cggcgtcgaa gccgcggagc tggcgggctg
      961 cgtcggtcgg tcgcaaccga gcgcactgga gagagcgcac atggccatga gatagaggct
     1021 tcggcttggt ttcatcggat gactctcctt tgggtcagta gaacgccggt gccgagcaat
     1081 ccgagcggaa ggacgagata ggcgagtagc gccaggagcg tgcgccgggt cccctcgagt
     1141 acgaacggaa aacccgggta tgaaggggac gaccagtcgg gcgcaggcat gttcgcggct
     1201 tcgaagatca gcgtggtcaa ctcgcgccgg ctggtggagc gcgtttcccg cgtgacgtcg
     1261 ccgacgtgca cttcgattcg gccgtagtcc gcatcgcgct ggccctcggc cacgagggtc
     1321 cgatcgtcga gaggcgtccg aacgacgagg tccggcctgg cgagataaag cttggcgata
     1381 acgtcggttt cgagctgcgt tcgcctcgaa tcatcgcggt cgagaaatac gtcgaggcga
     1441 agcggttgcc gaatgcgacg aaggccgagg gccgccgcgg gcgggagcga cgctcggcgt
     1501 tgctcgctcc agtcgtagcc gcggtggcta ccggcgacca agaagagcgt tacgcccgtc
     1561 acgagggcca ccagcaccac gacggtcgcc cgtcttacgc ccgacagatc gaagcgaaga
     1621 ccgacggccg caagcacgcc ggcgctcgcg atcgccgtcg cgaaccaa
//
LOCUS       DASUCJ010000743         1494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_236974, whole genome shotgun sequence.
ACCESSION   DASUCJ010000743 DASUCJ010000000
VERSION     DASUCJ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236974"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1494)
                     /locus_tag="VFU90_08905"
     CDS             complement(<1..>1494)
                     /locus_tag="VFU90_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5479937.1"
                     /translation="TKPAPAATDVTRATLTNGLRVVVVRDTLAPVVTTMLNYLVGSDE
                     QSIDGLAHAAEHMMFRGSATLSSSQLMDTMGITGGSFDADTQATVTQYFFTVPSQYLD
                     IALRVERSRAAGLTMAPDQWAQERGAITQEVTQDDSNALYRLFVKMERRIVAGTPYAK
                     NGLGTVQAFAGGVDSAQLRKFYATWYHPNNAVYVIVGDVDGPSTIAKVREFFSDVPAA
                     KLPARAAVRLQPLRGALYHDSSDQPYTAVLLGYRMPGYADPDYAAGQILADVLSSQRS
                     TFGGLAFTGKALGTQFFTQSFPQTAVGIAFAAVPVTTPPQTIDRELRAILDGYRRNGV
                     RADLVDAAKLREISELEFNGNSIQGLADEWSQAVAVQGLASPDDMIARFERVTPGDVD
                     RVLRKYLDNSTVVAAYAVPKNAGAASAGGGLHKENNAIPPSKHEPLPAWAQHVLDNLQ
                     VPQSTLNPTDVTLSNGMRLIVQPEHVTHTVVVSGEILNNADVQEPAG"
BASE COUNT          222 a          485 c          505 g          282 t
ORIGIN      
        1 tgcccggccg gttcctggac gtcggcgttg ttcaggattt ccccgctgac cacgacggta
       61 tgcgtgacgt gttcgggctg aacgatcagc cgcatgccgt tgctcagcgt gacgtcggtg
      121 gggtttagcg tcgactgcgg gacttgcagg ttgtcgagca cgtgctgcgc ccaggccggc
      181 aacggctcgt gtttgctcgg cgggatcgcg ttattttctt tgtgcaaccc gccgcccgcg
      241 ctcgcagctc cggcgttctt aggcaccgcg taggccgcca cgaccgtcga gttgtcgaga
      301 tacttgcgca gtacgcggtc gacgtcgccg ggcgttacgc gctcgaatcg cgcgatcatg
      361 tcgtcgggcg aggcgagccc ctgcaccgcg actgcctgac tccattcgtc ggccagccct
      421 tggatcgaat tgccgttgaa ttccagctcg gagatctcgc gcagcttggc ggcgtctacc
      481 aagtcggccc ggaccccgtt gcgccggtat ccgtcgagga tcgcacgcag ctcgcggtcg
      541 atcgtctgcg gcggggtcgt caccggaacg gctgcaaacg cgatgccgac cgccgtctgc
      601 ggaaagcttt gcgtgaagaa ctgcgtcccc aacgctttgc cggtgaatgc caggccgccg
      661 aaggtgctgc gctggctgga gagcacgtcg gcgaggattt gcccggccgc gtagtcggga
      721 tccgcgtaac cgggcatgcg atagccgagc agcacggcgg tgtaaggctg gtccgagcta
      781 tcgtgataga gcgcgccgcg cagcggctgc agccgtaccg cggcgcgcgc gggcaacttc
      841 gcggccggta cgtcgctgaa aaactcgcga actttggcga tcgtcgacgg tccgtcgacg
      901 tctccgacga tcacgtacac cgcgttgttc gggtgatacc acgtcgcgta gaacttgcgc
      961 agttgcgcgc tgtcgacgcc gccggcgaac gcctggaccg ttcccagccc gttcttcgca
     1021 tacggggtac cggcgacgat ccgccgctcc atcttgacga agagccggta gagcgcgttg
     1081 ctgtcgtcct gcgtgacttc ttgggtgatg gcgccgcgct cttgcgccca ttgatcgggc
     1141 gccatcgtaa gcccggccgc acgcgatcgc tcgacccgca gcgcgatgtc gaggtactgc
     1201 gagggcaccg taaagaagta ctgcgtcacc gtcgcttgcg tgtcggcgtc gaagctcccg
     1261 ccggtgattc ccatcgtgtc catcagctgc gacgacgaga gggtcgcgct gccgcggaac
     1321 atcatgtgct cggcggcgtg ggccagaccg tcgatcgact gttcgtcgga accgaccaga
     1381 tagttgagca tcgtcgtcac gaccggggcg agcgtatcgc gcaccacgac cacgcgcagg
     1441 ccgttcgtca gcgtcgcgcg cgtgacgtcg gtagcggccg gcgccggttt cgtc
//
LOCUS       DASUCJ010000744         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1276486, whole genome shotgun sequence.
ACCESSION   DASUCJ010000744 DASUCJ010000000
VERSION     DASUCJ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276486"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFU90_08910"
     CDS             complement(<1..545)
                     /locus_tag="VFU90_08910"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5479938.1"
                     /translation="MTRAISRRIAALILGVGCALNAAVPAPAQTLNIPVESHIRPIQT
                     AILFHEFFADFFPEKDGTTYVQTGDIPAMWLRDSAAQTIPYIRFVASYPALRYEFFGV
                     VQRDAKNILVDPHAEAFRADYTVWEGKWEIDSLAWPVVLAFMYYANTHDRTIFTPVLH
                     RALQVIVSTLQCEQHHATCSR"
     gene            647..1633
                     /gene="meaB"
                     /locus_tag="VFU90_08915"
     CDS             647..1633
                     /gene="meaB"
                     /locus_tag="VFU90_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5479939.1"
                     /translation="MANPNSGEELLRAFEARNPRALARAISWAEAGRGAELIRAVYAR
                     TGRAMTIGLTGPPGVGKSTLSSALVRKARALGRSVGVISVDPTSPFSHGALLGDRIRL
                     TEHFTDRDVFIRSMASRGHLGGLAGATADAALLMDAFGLDVVLIETVGVGQSEIEIAE
                     LAHTTLVALQPGSGDSIQVLKAGIMEIADIFVVNKSDHPMANQLRREIRSMMEMLAFP
                     GWVPDLVMTQALTGEGIDRLWEAIERHVAFLHESGEIAEKRRQAFLHQVRQLALGRLS
                     HRLDGVLREAAGGSDPYAAADRVLATFGGAGEDADVEDAPSLRPRPTVKGLT"
     gene            1725..3428
                     /locus_tag="VFU90_08920"
     CDS             1725..3428
                     /locus_tag="VFU90_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5479940.1"
                     /translation="MIDRPSGLGAPVTPEFEEQKRRWERASAKGKPRKGAGSGAVDRT
                     ISDVPLQTAYGPEDVAHLDLARDLAWPGEFPYTRGIHPNGYRDRLWTMRQFAGFGTAQ
                     QTNERYHFLLEQGQHGLSVAFDMPTLMGYDSDAPQARGEVGKCGVAIDSLADMETLFA
                     GIDMADITTSMTINGPAAIALAQYVAAAEKKGIPRAKLGGTIQADILKEYIAQKEWIF
                     PPRPHMRVIVDMIDFCTRELPRWNTISISGYDIREAGSTAAQELAFTLADGFAYVEAC
                     MAAGMDVDSFAPRLSYFFNSHIDFFEEIAKFRAARRIYARHMRDKYGAKNPKSWQLRF
                     HTQTAGCSATAQQPENNIVRVAFEAMAAVLGGTQSLHTNSMDEVLALPTEKSVEIALR
                     TQQVLAYETNVTNVVDPLGGSYYVEALTDEMERQAEAYFAQIAEYGGVVEAIEAGFFQ
                     KEIAEASYRYQRSIESKDRVVVGVNGFVRDGETVEIDLLRISEEMEREQARAVQDVRS
                     GRDDSAAQKTLERLQRACRDPKDNLMPHLIDCVNAYCTEGEIVDAMVQVYGRYTERSV
                     F"
     gene            3559..3975
                     /locus_tag="VFU90_08925"
     CDS             3559..3975
                     /locus_tag="VFU90_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5479941.1"
                     /translation="MKKRPLRIVIAKAGLDGHDRGAKVIARALRDAGMEVIYTGLFQA
                     PEQIVQTAIQEDADGIGLSILSGAHMTLFPLVLEQLRAQGADDIVFFGGGTIPPDDAL
                     ELKRLGVREIFTPGAPLKQIVDFVDRECGRRRELVG"
BASE COUNT          747 a         1290 c         1328 g          662 t
ORIGIN      
        1 taacggctgc aggttgcgtg gtgctgctcg cattgcaagg tcgacacgat gacttgcagc
       61 gcccgatgga ggacgggcgt aaagatcgtg cgatcgtggg tgttggcgta atacatgaac
      121 gccaggacca ccggccacgc aagcgagtcg atctcccact tgccctccca gaccgtgtag
      181 tcggcacgga acgcctccgc gtgcgggtcg accagaatgt tcttggcgtc gcgctggacg
      241 acgccgaaga actcgtaacg aagcgccgga tagctcgcga cgaagcggat gtacgggatc
      301 gtttgagcgg cggagtcgcg cagccacatc gccgggatgt cgcccgtctg cacgtacgtc
      361 gtcccgtctt tctcgggaaa gaagtcggca aaaaattcgt ggaagagaat cgcggtttga
      421 atcggtcgaa tgtgcgattc gacgggaatg ttcagcgttt gcgcgggggc cggaacggcg
      481 gcgttcaatg cgcagcccac gccgagtatc agcgcggcga ttcgtcgcga gatcgctctc
      541 gtcatgtcgc cgggaacttc gcctcgcggt ttgcggcgaa cctcttcgcg gcgtcgtcgg
      601 gtcgcaggag gttcgccgtc gctcgtgccg caatgatgca aggactttgg cgaatccaaa
      661 ctccggcgaa gaactcctgc gagcgttcga agcgcgcaat ccgcgcgcgc tggcccgtgc
      721 gatttcgtgg gcggaagccg gtcgcggtgc ggagttgatc cgcgcggtct acgcgcggac
      781 cggccgcgcg atgacgatcg gcctcaccgg accgccgggc gtcgggaaat cgacgctttc
      841 gtcggcgctg gttcgcaagg cgcgcgccct cggaagaagc gtcggcgtca tttcggtcga
      901 tcccacgtcg ccgttctcgc acggtgcgct gctcggagac cgcatccgcc tgaccgaaca
      961 cttcaccgat cgcgacgttt tcattcgttc gatggccagc cgcgggcact tgggcggcct
     1021 ggccggcgcc accgcggacg cggcgctgct gatggacgcg ttcggtctcg acgtcgtgct
     1081 gatcgagacg gtcggcgtcg ggcagagcga gatcgagatc gcggagctcg cgcacaccac
     1141 gctggtggcg ctccagccgg gaagcggcga ctcgatccaa gtgctcaagg cgggaatcat
     1201 ggagatcgcc gacatcttcg tggtcaacaa atccgaccac ccgatggcca atcagctgcg
     1261 ccgcgagatt cgctcgatga tggaaatgct cgcgttcccc ggctgggtgc ccgacctcgt
     1321 tatgactcag gcccttaccg gggaaggaat cgaccggttg tgggaggcga tcgaacggca
     1381 cgtggcgttc ttgcacgaaa gcggcgagat cgccgaaaag cgacgccaag catttttgca
     1441 ccaggtgcgc cagctcgcgt tggggcggct gtcgcaccgg ctggacggcg tgctgcgcga
     1501 ggccgccggc ggctccgacc cgtacgctgc cgcggatcgt gttcttgcga ctttcggcgg
     1561 agccggcgag gacgccgacg tcgaggatgc gccgtccctg cgaccgcgcc cgaccgtgaa
     1621 gggtctcacg tagcggcgtt ggggagaatc gggtgcgtcc gtcgaagcgc cccgcttcgg
     1681 aatcggatgc aatcccgaga ataggaaacc gtgacttggc caaaatgatc gatcgcccga
     1741 gcggcctggg cgcgccggtg acgccggagt tcgaggaaca gaaacgccgc tgggagcggg
     1801 cctcggccaa aggcaagccg cgcaaaggcg cgggttcggg cgccgtcgac cggacgattt
     1861 ccgacgtgcc gctccaaacg gcgtacggcc ccgaggacgt cgcgcatctc gacttggcgc
     1921 gcgacctcgc ctggccgggc gaatttccgt acacgcgcgg catccatccg aacgggtatc
     1981 gcgatcgctt gtggacgatg cggcagttcg ccggattcgg caccgcacag cagaccaacg
     2041 aacgctatca ctttctgctg gaacagggcc agcacggcct ctccgtcgcg ttcgatatgc
     2101 cgacgctgat gggatacgac tccgacgcgc cgcaggctcg cggcgaggtc ggcaagtgcg
     2161 gcgtggcgat cgattcgttg gccgacatgg agacgctctt cgccggcatc gacatggccg
     2221 acatcaccac ctcgatgacg atcaacggtc cggccgcaat cgcgctcgcc caatacgtcg
     2281 ccgccgccga gaagaagggc atcccgcgag ccaagctcgg cggcacgatt caagccgaca
     2341 tcctcaaaga gtacatcgcg cagaaggaat ggatcttccc gccgcgtccg cacatgcgcg
     2401 tgatcgtcga catgatcgat ttctgcacgc gggaactgcc gcgctggaac acgatctcga
     2461 tttccggcta cgacatccgc gaggcggggt cgacggccgc gcaagaactg gcgttcaccc
     2521 tcgccgacgg ctttgcgtac gtcgaagcct gcatggccgc cggtatggac gtcgactcgt
     2581 tcgcgccgcg cctgtcgtac tttttcaact cccacatcga cttcttcgag gagatcgcca
     2641 agttccgcgc ggcgcggcgc atttacgcgc gccacatgcg cgacaagtac ggcgcgaaga
     2701 atccgaaatc gtggcagctt cgcttccaca cgcaaaccgc cggctgcagt gccaccgccc
     2761 agcagcctga gaacaacatc gtgcgggtcg ccttcgaggc gatggcggcg gtgctcggcg
     2821 ggacgcaatc gctccacacg aactcgatgg acgaagtttt ggcgctgccc accgaaaaat
     2881 cggtcgagat cgcgctgcgc acccagcaag tgctggcgta cgaaacgaac gtcaccaacg
     2941 tcgtcgaccc gctcggcggt tcgtactacg tggaagcgct gaccgacgaa atggaacgcc
     3001 aagccgaggc ctatttcgca cagatcgccg agtacggcgg cgtggtcgaa gccatcgagg
     3061 ccggcttctt ccaaaaggaa atcgccgagg cgagctatcg ctaccagcgc tcgatcgaaa
     3121 gcaaggatcg cgtcgtcgtc ggcgtcaacg ggttcgtacg cgacggcgaa accgtcgaga
     3181 tcgacctgct gcggatcagc gaggagatgg agcgcgaaca ggcgcgcgcc gtgcaggacg
     3241 tgcgttccgg tcgcgacgac tccgccgcgc aaaagacgct ggagcggctc cagcgcgctt
     3301 gccgcgatcc caaggacaat ctgatgccgc acctgatcga ttgcgtcaac gcctactgca
     3361 ccgaaggcga aatcgtcgac gcgatggtcc aggtttacgg tcgctacacc gaacgctcgg
     3421 tgttctaaga aacgaggcaa ccccgcatgc gcttcgtaac cgaactcgaa cggcaccgcg
     3481 tgcgtcgcac gctcgcggag gtacggcgca aactggagga agcgctgcgc ctgagccgat
     3541 tgcgacacga aaggaaccat gaagaagcga ccgctgcgaa tcgtcatcgc taaggcgggt
     3601 ctcgacggcc acgaccgcgg cgccaaggtc atcgcgcggg cgctgcgcga cgccggcatg
     3661 gaagtgatct acacgggcct ctttcaggct cccgaacaga tcgtgcagac cgcgatccaa
     3721 gaagatgccg acggcatcgg cttgtcgatt ctctccggcg cgcacatgac gttgttcccg
     3781 ctggtgctcg agcagctgcg cgcgcaaggc gccgacgaca tcgtcttttt cggcggcggg
     3841 acgatcccgc ccgacgacgc gctggagctc aagcggctgg gcgtccgcga aatctttacg
     3901 ccgggcgcgc cgctgaagca gatcgtcgac ttcgtcgatc gcgagtgcgg ccgccggcgc
     3961 gaactcgtcg gctaggttct cccgatcttg gcgaaggtcc gcacgcgagt cgcgccctca
     4021 cccacgg
//
LOCUS       DASUCJ010000745         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1031979, whole genome shotgun sequence.
ACCESSION   DASUCJ010000745 DASUCJ010000000
VERSION     DASUCJ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031979"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFU90_08930"
     CDS             complement(<1..433)
                     /locus_tag="VFU90_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5479942.1"
                     /translation="MNFLLAAVLAAPSAFAPLWSAPHWIGAPPAAAAYAGRVVIVDIF
                     TVDCINCQHVVPELRRLRGKYGTRDLLVVGVHTPETPSERERSHVVHALTEQGITWPV
                     AIDNDEKLWNAFGAEYWPTQLIFDRHGTLQRTIVGEGSDDAV"
     gene            complement(466..>1127)
                     /locus_tag="VFU90_08935"
     CDS             complement(466..>1127)
                     /locus_tag="VFU90_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEU5479943.1"
                     /translation="LLNPVSYAEFSIYPKLVIPGQLEQTVANIAAQPTLFLVAAFCYL
                     ISFIGDVVIAWALYVLLAPVNAAVSLLAAWFRLIYAAMALTAWLNLVVVYRLVTTPEY
                     RSVFGARPLGAQIDLHLHTFRYGWSLSLLIFGSHLLLVGALIFRSAYVPRILGVILAI
                     DGLAWIVSVLQPYFYPNANVGFLFVAYFGELLFMLWLLIMGWRVPEPPIAHPQSDPAR
                     S"
BASE COUNT          244 a          342 c          372 g          169 t
ORIGIN      
        1 cgaccgcgtc gtcgctgcct tcaccgacga tcgtccgctg taacgtaccg tgacgatcga
       61 agatcagctg cgtcggccag tactcggcgc cgaacgcgtt ccagagcttt tcgtcgttgt
      121 cgatcgcaac cggccacgtg atgccttgct ccgtcagagc atggacgacg tgcgatcgct
      181 cgcgctcgga gggcgtctcg ggcgtgtgca cgccgaccac gagcaggtcc ctcgttccgt
      241 acttgccgcg caaccggcgc aactccggca cgacgtgttg gcagttgatg cagtccaccg
      301 taaagatgtc gacgatcacg acccggccgg catacgccgc cgcggccggc ggcgctccga
      361 tccagtgcgg tgcgctccag agcggcgcga acgcgctcgg tgccgccagg acggcggcca
      421 gtaagaagtt catcgaagta gcggggatgg atcgagcttc atgttctaag aacgcgcggg
      481 atcgctttgc ggatgagcga tcggcggttc cggaacccgc cagcccataa tcagcagcca
      541 cagcatgaac aacagctcgc cgaaataggc gacgaagaga aagccgacgt tcgcgttcgg
      601 atagaaatac ggctggagga cgctgacgat ccaggcgagc ccgtcgatcg ccaagatgac
      661 gccgaggatc cgcgggacgt aggccgatcg gaagatcagg gcgccgacga gcagcaaatg
      721 acttccgaag atcagcagac tcagcgacca gccgtagcgg aacgtatgca gatggaggtc
      781 gatctgcgcg ccgagcggcc gggcaccaaa gacggaccgg tactccggcg tcgtcaccag
      841 gcggtagacg acgacgagat tgagccaagc ggtcagcgcc atcgccgcgt atatcagccg
      901 gaaccatgcc gccagcaacg acaccgccgc gttgaccggc gcgagcaaga cgtagagcgc
      961 ccaggcgatg accacgtcgc cgatgaagct gatgagatag caaaacgctg ccacgaggaa
     1021 gagcgtgggt tgagcggcga tattcgcgac cgtctgttcg agctgccccg ggatgacgag
     1081 tttcgggtag atggagaact cggcatacga aacgggattg agcaggt
//
LOCUS       DASUCJ010000746         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_741617, whole genome shotgun sequence.
ACCESSION   DASUCJ010000746 DASUCJ010000000
VERSION     DASUCJ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741617"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFU90_08940"
     CDS             complement(<1..820)
                     /locus_tag="VFU90_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479944.1"
                     /translation="MSAQPTLVVIGASGDLAARLLFPALLKLEHRERLEDLKVIGYAR
                     QEWTTDQFHENLRAAIDVHGNDVDQKYVDRFIDRIEFHGGDISVESMRQLGGVLSGPA
                     IFYLALPPGVFADAAETIAKAGLADETNGWRRLVIEKPFGTDLESALALNEQLHRYWR
                     EDQIFRIDHFLGKETVQNMLVFRFANRFIEPVLKSAHVDEIQITVAETLGVEGRSRYY
                     DGIGAIRDMIQNHLIQMMTFATLEPPALWDSEMLRDHKVEVLKSVQPVDTERDAV"
     gene            complement(817..>1081)
                     /locus_tag="VFU90_08945"
     CDS             complement(817..>1081)
                     /locus_tag="VFU90_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5479945.1"
                     /translation="AGVVVRGRHAGGYGFPSGHTAVAFAVAAALAPDLPRPVRPFVWL
                     LAVIVGFARVYVGAHFPLDVLGGAALGYAIGSGARLAFRNLAR"
BASE COUNT          203 a          356 c          348 g          174 t
ORIGIN      
        1 gcaccgcgtc gcgctcggta tcgaccggtt gcaccgactt caacacttcg accttgtggt
       61 cgcgcagcat ctcggaatcc cacaacgcag gaggttcgag cgtcgcgaag gtcatcatct
      121 ggatgagatg gttctggatc atgtcgcgaa tcgcgccgat gccgtcgtaa tatcgcgaac
      181 ggccctcgac gccgagcgtt tcggcgacgg tgatttgaat ctcgtcgacg tgcgccgact
      241 tcaggaccgg ctcgatgaag cggttcgcga agcggaacac caacatgttc tgcacggtct
      301 ctttgccgag gaaatggtcg atgcggaaga tctggtcttc tcgccaatac cgatgtagct
      361 gttcgttgag cgcgagcgcg ctttcgagat cggtcccgaa cggcttctcg atcacgagcc
      421 gtcgccaacc gttcgtctcg tcggccagac ccgccttcgc gatcgtctcg gccgcatcgg
      481 cgaagacgcc gggcggcaac gcgagataga agatcgccgg cccgctcaag acgccgccca
      541 actgtcgcat cgactcgacc gagatgtcgc caccgtggaa ctcgatccgg tcgatgaacc
      601 gatccacgta cttctggtcg acgtcgttgc cgtgcacgtc gatcgcggcg cgcaggttct
      661 cgtggaactg atcggtcgtc cactcctggc gggcatagcc gatcaccttg aggtcttcga
      721 gccgctcgcg gtgctcgagc ttcagcaacg cggggaacag cagccgagcc gcgagatcac
      781 ccgaggcccc gatcacgacg agtgtcggtt gcgcgctcat cgggccaggt tacggaacgc
      841 gagccgagcg ccgctgccga tcgcgtatcc gagcgcagca ccgccgagca cgtcgagcgg
      901 gaagtgcgcg ccgacgtaga cgcgggcgaa gccgacgatg accgcaagca gccacacgaa
      961 cgggcgaacc ggccgcggga gatcgggcgc gagcgccgcg gcgaccgcaa acgcgacggc
     1021 cgtatgaccc gacggaaagc cgtagccacc ggcatggcgc ccgcgcacga cgacaccggc
     1081 g
//
LOCUS       DASUCJ010000747         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_389995, whole genome shotgun sequence.
ACCESSION   DASUCJ010000747 DASUCJ010000000
VERSION     DASUCJ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFU90_08950"
     CDS             complement(<1..1241)
                     /locus_tag="VFU90_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5479946.1"
                     /translation="MPYALFSGPGSLRDPAERIAFWLTAVCALPAALLIGYVLHESIG
                     ASQVALFIVIAMVYVTLARGRLLGSSVRVHETQYPRVFAIVKNACAALEIPMPLVFVR
                     EDNYVPVAALGFGEPYALVLSSHWIEAFRDDELAFMIGRELGHIAAGHTRFLSLLSVN
                     GKENPLISLIFGGWLRRCTMTCDKVGLLCCGTLDAAIRAIGVAEFHEFGRKVDYQAFA
                     EQHEEIEADTISRWGVWLGSEPYATTRIAALRRFNVSQAFVDAEQWFLRERGEEPPGL
                     AEPGAATVSSRDCAGWWRRFAAVSIDAVVVLAIINSFGGSGSPAPVYVRVDDSAPAPS
                     ASPHRSRRPVATPAPTAPPATAAPSAAPGTDQEIIGPLVFNDQTGPRLRIGSLVITPN
                     VADPNRSSGKTIWFAIYMTLLV"
BASE COUNT          294 a          518 c          517 g          244 t
ORIGIN      
        1 accagcagcg tcatgtaaat tgcgaaccag atcgttttgc cgctgctgcg attcgggtcc
       61 gcgacgttcg gcgtgatgac gaggctgccg atgcgcaagc gcggacccgt ttgatcgttg
      121 aacacgagcg gtccgatgat ctcctggtcg gtgccgggcg cggccgacgg tgcggccgtc
      181 gccggcggcg ccgtcggagc cggcgtcgcg accggccggc gcgatcgatg cggcgacgcg
      241 ctcggcgccg gagcgctgtc gtcgacgcga acgtacaccg gcgccgggct gccgctgccg
      301 ccgaacgagt tgatgatcgc gagcaccacg accgcgtcga tcgaaaccgc cgcgaaccgt
      361 cgccaccagc cggcgcagtc gcgcgacgat accgttgcgg cgccgggttc cgcgaggccg
      421 ggcggctcct cgccgcgctc gcgcaagaac cactgctccg cgtcgacgaa cgcttgcgac
      481 acgttgaagc gccgcagcgc ggcgatgcgc gtggtagcgt acggctcgct gccgagccac
      541 actccccagc gcgatatcgt atcggcctcg atctcctcgt gctgttcggc aaacgcttga
      601 tagtcgactt ttcttccgaa ctcgtgaaac tcggcgacgc cgatcgcgcg aatcgccgcg
      661 tcgagcgtgc cgcagcacag caatcccact ttgtcgcagg tcatcgtgca gcggcgcagc
      721 catccgccga agatcagcga gatcagcgga ttctctttcc cattgacgct cagcagcgag
      781 agaaagcggg tgtgaccggc ggcgatgtgt ccgagctcgc gcccgatcat gaacgccagc
      841 tcgtcgtcgc gaaaggcttc gatccagtgg ctcgacagca ccaaggcgta cggttcgccg
      901 aaacctaggg ctgccaccgg aacgtagttg tcttcacgca cgaacaccag cggcatggga
      961 atctcgagcg cggcgcaggc gttcttgacg atcgcaaaca cgcgcggata ctgggtctcg
     1021 tgcacgcgca cgctcgatcc gagcagccgc ccgcgcgcca gcgtgacgta aaccatggcg
     1081 atgacgatga acagtgccac ttgcgaagcc ccgatcgatt cgtgcagcac gtagccgatc
     1141 agcagggcgg cgggaagcgc gcagacggcg gtcagccaga aggcgatgcg ttcggccgga
     1201 tcgcgcagcg agcccgggcc ggagaagagc gcgtagggca ccagcacctt ctgggaggcg
     1261 gcaggccgcc tttccttcgt ggagggggat tgacggcgtc ccccgattgc gctattgtac
     1321 tagtgtacta gtacgataag agattggtac taggcacccg aagaaggaac caaccaaaat
     1381 gacgtacatg accatcggcc gcaccgacga gaacgcccgc gaagcgcgga ccacggtacg
     1441 cgtgcccgac caactgggaa cccgactgtt ccaaggctgg ggcccgaagt tctaggaggc
     1501 cccagttcga catgccggcc gtactgacgg tcgacccgcg tagcggcgtt ccgctctacc
     1561 tgcagatcat cga
//
LOCUS       DASUCJ010000748         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_894515, whole genome shotgun sequence.
ACCESSION   DASUCJ010000748 DASUCJ010000000
VERSION     DASUCJ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894515"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFU90_08955"
     CDS             complement(<1..859)
                     /locus_tag="VFU90_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEU5479947.1"
                     /translation="MRCVLMCPPDYFTIRDVKNPFMQSAAPIDRRRAFAQWRALRDAF
                     ERAGLCVATIEAVEDLEDMTFAANPVFAGTGRVHERFVVPSRMRYASREREVAHYVAW
                     FARRGFAVLDARLGEGDYLEGFGDLLPHPERAVIWAGYGYRSSHGGIARVASTLRPEG
                     LHVEPLRLADPTFYHLDTCLAPLNENAALCFFPAFAPDSLAALRRGWPRLYDVPRDDA
                     LRFACNGIVAGERYVVSHASSWLRATLADEGLTPLAIDVSEFEKSGGSVFCLKASFED
                     APDFKREATG"
     gene            complement(856..>2590)
                     /locus_tag="VFU90_08960"
     CDS             complement(856..>2590)
                     /locus_tag="VFU90_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEU5479948.1"
                     /translation="IVAPGIFPAGFRTMGGTVPVYFEAAAVITVLVLLGQVLELRARE
                     RTGDAIRALLNLAPKTAHRVRSDGDDEVVPIDAIAIGDRLRVRPGESVPVDGVVLDGA
                     GAVDESMVTGEPIPVAKAAGDTLVGGTVNGTGSLVMQAQRVGAETMLARIVAMVAEAQ
                     RSRAPIARLADTVSSYFVLAVVAAAAVAFVCWAVWGPPPSLAYALIVAVSVVIVACPC
                     ALGLATPMSIGVAVGKGATAGVLIRSAQALERLERAGVLVVDKTGTLTEGKPRVVATY
                     AVDGGAGDELLALAASLERSSEHPLAQAVLAAARERGLPISDAAGFASSTGKGISGRV
                     GAIEVALGNAAMMAGAGIALGDLDAKAEEFRRDGATVLFVSAAGRAAGLIAVADPLKT
                     TARAAIETLVRDGMAVVMLTGDNATTAGAVARSLGIEEVHAEVLPEQKQAIVERIQAG
                     GKVVAMAGDGINDAPALAAADVGIAMGSGTDVAIASAGITLVKGDLQGIVRARALSRA
                     TMRNVRENLTFAFGYNALGIPIAAGVLYPAFGLLLSPAVAALAMSFSSVSVIANALRL
                     RRLRLPGTRVR"
BASE COUNT          453 a          914 c          847 g          376 t
ORIGIN      
        1 tgcctgtcgc ttcgcgtttg aaatccgggg catcttcgaa ggacgccttc aggcaaaaga
       61 cgctgccgcc ggatttttcg aactccgata cgtcgatcgc caaaggagta agcccttcat
      121 ccgccagcgt cgcccgcagc cacgacgatg cgtgcgaaac gacgtaacgt tcgccggcga
      181 cgatgccgtt gcaggcgaac cgcagcgcgt cgtcgcgcgg aacgtcgtag agacgcggcc
      241 acccgcgacg gagggcggcg agcgagtccg gcgcaaatgc cgggaaaaag cacagcgccg
      301 cattttcgtt aagcggcgcc agacacgtgt cgaggtggta gaacgtcgga tcggctaatc
      361 gcaacggctc gacgtgcagc ccttcgggcc gcaacgtgct tgcaacccgc gcgatgccgc
      421 cgtgcgacga tcggtagccg taaccggccc agataaccgc gcgctccggg tgcggcagca
      481 ggtcgccgaa tccttcgaga tagtcgccct cgccaagccg cgcatcgagc accgcgaatc
      541 ccctgcgcgc gaaccacgct acgtagtgcg ccacctcgcg ttcgcgcgag gcgtatcgca
      601 tccggctcgg tacgacgaaa cgttcgtgca ctcggccggt cccggcgaaa accgggttgg
      661 cagcaaacgt catatcctcg agatcttcga cggcctcgat cgtcgcaacg cacaggccgg
      721 cacgctcgaa ggcgtcccgc aacgcgcgcc attgcgcaaa ggcgcgccgg cgatcgatcg
      781 gcgcggccga ctgcatgaac ggatttttca cgtcccggat cgtgaagtaa tcggggggac
      841 acatcaagac gcatctcatc ggacgcgggt tcccgggagg cggagccggc gtaagcgcag
      901 cgcgttggcg atcacggaaa cggagctgaa cgacatcgcg agcgccgcga ccgcggggct
      961 gagcagcagg ccgaacgcgg gatacaggac gccggcggca atcgggatcc ccagcgcgtt
     1021 gtatccgaac gcgaaggtca agttttcgcg aacgttgcgc atcgtcgccc ggctcagcgc
     1081 gcgcgcgcgg acgatacctt gaaggtcgcc tttgaccagc gtaatcccgg cgctggcaat
     1141 cgcgacgtcg gttccactgc ccatcgcgat cccaacgtcg gccgccgcga gcgcgggtgc
     1201 gtcgttgatg ccgtcgccgg ccatcgccac gactttaccg ccggcttgga tgcgttcgac
     1261 gatggcttgc ttctgctcgg gcagaacctc ggcgtgaact tcctcgattc cgagcgaccg
     1321 cgcgaccgcg ccggccgtcg ttgcgttgtc gccggtgagc atgacgaccg ccatgccgtc
     1381 gcgcacgagc gtctcgatgg ccgcgcgagc cgtcgtcttg agcggatcgg ctaccgctat
     1441 taggccggcc gcccggccgg cggccgacac gaacagcacg gtcgcaccgt cgcggcggaa
     1501 ttcctcggcc ttcgcatcga gatcgccgag cgcgataccg gcgccggcca tcattgcggc
     1561 gttcccgagc gcgacctcga tcgctccgac gcggccggag atcccctttc cggtcgacga
     1621 cgcaaacccg gctgcgtcgg aaatcggaag gccccgttcg cgtgccgcgg cgagcaccgc
     1681 ttgcgccagc ggatgctcgc tcgagcgttc gagcgatgcg gccagcgcga gcagctcgtc
     1741 accggccccg ccgtcgacgg catacgtcgc gacgacgcgc ggcttgcctt cggtaagcgt
     1801 gcccgttttg tcgacgacca gcacgccggc gcgctccagc cgttcgagcg cttgcgccga
     1861 tcggatcagc acgcccgcgg tcgcgccctt gccgacggca acgccgatcg acatcggcgt
     1921 ggccaatccg agcgcgcacg gacaggcgac gatcaccacc gataccgcga cgatcagcgc
     1981 gtaagccagc gacggcggcg gaccccaaac ggcccaacaa acgaacgcaa cggcggcggc
     2041 ggcgacgacg gccagaacga aatacgacga taccgtgtcg gccagccgcg cgatcggtgc
     2101 ccggctgcgc tgcgcctccg cgaccatcgc gacgatccgc gcgagcatcg tttccgcgcc
     2161 gacgcgctga gcttgcatga cgagggaacc cgttccgttg accgttccgc cgacgagcgt
     2221 atcccccgcc gccttcgcga ccgggatcgg ctcgccggtc accatcgatt cgtcgaccgc
     2281 gccggcaccg tccagtacca cgccgtcgac cggcacgctc tcgccggggc gcacgcgcaa
     2341 gcggtcgccg atcgcaatcg cgtcgatcgg aacgacctcg tcgtcaccgt cggagcggac
     2401 gcgatgcgcg gttttgggag cgagattcag cagcgcgcgg atcgcgtcgc cggtccgttc
     2461 gcgcgcgcgc agctcgagca cctgtccgag cagaacgagc accgtaatga ctgctgcggc
     2521 ttcgaagtag accggtaccg taccgcccat cgtgcggaag cccgccggaa aaatacccgg
     2581 ggcgacgatc
//
LOCUS       DASUCJ010000749         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_420568, whole genome shotgun sequence.
ACCESSION   DASUCJ010000749 DASUCJ010000000
VERSION     DASUCJ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420568"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFU90_08965"
     CDS             complement(<1..637)
                     /locus_tag="VFU90_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479949.1"
                     /translation="MPKIVTPSGIHQTTAQRNLAEAGTIPRNGKARAVPVVLADFERV
                     VMSPLCPQDELKQIQVHFMTIAQCAKRGITSERERAELNASIQEKMARYGASSRHIAA
                     RQVGVLHSPFVTHDPAATGPKKAYGDLRVATGELLDARMSLFEETACAVLDRVYAGET
                     DAPDGVVHATCSGYVSPSPVQVFLSRRRWLTTDVTHSYHMGCYGAFPAVRTA"
     gene            complement(789..>1073)
                     /locus_tag="VFU90_08970"
     CDS             complement(789..>1073)
                     /locus_tag="VFU90_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5479950.1"
                     /translation="YQGLGFRVPMLVISPYAKTVGYISHTPYEFGSILRFIEQNWNLK
                     PLGTTDVRAASIADMFDFSRSPRAFAPAPQSLPPAYFESRPASIDPPDDE"
BASE COUNT          182 a          320 c          357 g          214 t
ORIGIN      
        1 aggcggtgcg aaccgccgga aacgccccgt agcatcccat atggtaggaa tgcgtcacgt
       61 cggtggtgag ccagcggcgt cgcgacagga aaacctggac cgggctgggg gagacgtacc
      121 ccgagcacgt cgcgtggacg acgccgtcgg gtgcgtccgt ttcgccggcg taaacgcgat
      181 cgagcaccgc gcacgccgtc tcttcgaaga ggctcatgcg cgcgtcgagc agctcgccgg
      241 tcgcgacccg cagatcgccg taggcctttt tgggtccggt tgcggcgggg tcgtgcgtca
      301 cgaacggcga gtggagcacc ccgacttggc gcgcggcgat gtgacgcgac gacgccccat
      361 agcgcgccat tttttcttga atcgaggcgt tgagctcggc ccgctcgcgt tcggaggtga
      421 tcccgcgttt ggcgcactgc gcgatcgtca tgaagtgcac ctgaatttgc ttgagctcgt
      481 cttgcgggca cagcggcgac atcacgacgc gttcgaagtc ggcgagcacg acgggaaccg
      541 cgcgcgcttt accgttccgt ggtatggtgc ccgcttcggc gaggttgcgc tgcgctgtcg
      601 tttggtggat acccgatgga gtcacgatct ttggcataaa tatttcggtg ctggtgaggc
      661 tcctcttcag aaacaacggc gtgcgctgaa gcggtgccta ggttgcgtta cctttcccca
      721 gcctcgcacc cttccatttc gcgagcgatt ttcatgcttt ctgcggggac gagtaccacg
      781 gtcacccttt attcgtcgtc gggcggatcg atcgatgcgg gccggctttc gaagtaggcc
      841 ggcggcagcg attgcggggc cggcgcaaac gcgcgcggcg acctgctgaa atcgaacatg
      901 tcggcaatgc tggccgccct gacgtcggtc gttcccaacg gcttcaggtt ccaattctgc
      961 tcgatgaagc gaaggatgct tccgaactcg tagggcgtat gcgagatata gcccacggtc
     1021 tttgcatacg gcgagatgac gagcatcgga acgcgaaacc cgaggccctg ata
//
LOCUS       DASUCJ010000750         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_650055, whole genome shotgun sequence.
ACCESSION   DASUCJ010000750 DASUCJ010000000
VERSION     DASUCJ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650055"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFU90_08975"
     CDS             complement(<1..805)
                     /locus_tag="VFU90_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479951.1"
                     /translation="MKTACILGVALFASGVAVEAAAYTFTTCGATGAQGPTQAQCDTA
                     YGGAITVTVNGGIQSWTVPVSGTYHIVATGAQGGSGSLPSFVGGRGAQVSGDFALTAG
                     QVLKLVVGQAGTNGGTENGGGGGGSFVVSSSNAAMLIAGGGGGIRAGASQNGCDASLT
                     QFAVTGSGGSPTSTCALEATDLGLGGIVSSGSWGSAGAGFNGDGATDFGTHSGGYSWA
                     NGILGGTTDETCSTSAPGGFGGGGAGNGCWGGGGGGGYSGGDGGWIAGGG"
     gene            1050..1826
                     /locus_tag="VFU90_08980"
     CDS             1050..1826
                     /locus_tag="VFU90_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5479952.1"
                     /translation="MPAAFPATNAAFARSAADPIVAVAAPVVFVARNLSKIYRMGEVE
                     VHALRAVDLEIREREFIVLLGPSGSGKSTLLNILGGLVVPSGGEALWRDHDLAHADEA
                     MLTRYRRDHVGFVFQFYNLIPSLTVSENVALVTDIVENPLPVDEAVARVGLSERAHHF
                     PSQLSGGEQQRVAIARAIAKRPDVLLCDEPTGALDYATGKIVLEAIANINRDLGTTTV
                     VITHNAPIAQMADRVLRLADGRIVGEDRNERKRSPGEISW"
     gene            2003..>2976
                     /locus_tag="VFU90_08985"
     CDS             2003..>2976
                     /locus_tag="VFU90_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5479953.1"
                     /translation="MELKRAPLALIRDFERIPGVAAVQATVVFDVVLDVPNVAEPVTG
                     RLIGLANGDASTLDAVVIRRGRMPEPRSPSEVVVSEGFATARGLAPGASLKALINGKL
                     QTLQVVGIGLSPDYIYATRGGAFPDDRNFGVLWMARESLAAAYDMEGAFNHLALRLAP
                     GAPERAVLDAADRLLRRYGGTRAYGRDQQASHRILGQEIDQWKVIGALIPSIFLAVSA
                     FLLNVVLGRQVATQRGQIAALKALGYGNRALLRHYLLQVVAIVTIGVAIGVGIGWWFG
                     SAVTRLYADFFHFPSYRFSIPAWVLLVAGAITFGAGMSGAIIAVRKTVL"
BASE COUNT          448 a         1080 c          933 g          515 t
ORIGIN      
        1 cacctccgcc ggcgatccat ccgccgtcgc cgccggagta accaccacca ccgccaccac
       61 cccagcaacc gttaccagcg ccaccgccgc cgaaacctcc gggcgcgctc gtgctgcagg
      121 tctcgtcggt cgttccgccg agtatgccgt tcgcccatga atagccgccg ctgtgggtcc
      181 cgaaatcggt cgcgccgtcg ccgttgaaac ccgcgcccgc cgaaccccag ctgccgctcg
      241 agacgatccc gccgagaccg agatccgtag cctcgagggc acaggtcgac gtcgggctgc
      301 cgccgctgcc ggtcaccgcg aactgcgtca gcgacgcatc gcacccgttt tgactggcgc
      361 cagcccgtat tccgccgccg ccgccggcga tcagcatggc ggcattcgag ctgctgacga
      421 cgaagctccc gcctccgccg ccaccgttct cagtgccacc gttcgtgcct gcctgcccga
      481 caacgagctt cagcacctgc cccgcggtga gtgcgaaatc tcccgatacc tgcgcgccgc
      541 ggccaccgac gaaactgggc aagctacccg aaccgccctg cgcgccggtc gcgacgatgt
      601 ggtaggtccc gctcaccgga acggtccacg actggatccc gccgttcacc gtgacggtga
      661 tcgctccgcc gtaggcggta tcgcactggg cctgcgtcgg gccctgagct ccggtcgcgc
      721 cgcaggtagt gaaggtgtag gccgctgcct cgaccgcaac gccgctcgcg aagagcgcca
      781 ctcccaggat gcacgctgtt ttcatcgctt ccccgatcat tattgtggtt cggtcccgcg
      841 ttcgactgtg gagtatccgc gggcggcgac atggcgacaa cactgcgcgt cgaaattcgg
      901 ctccacgaga caggctatgt tgcgtcgccg ccaatagcgg ccgatggccg gccacgggac
      961 cgggcagcct tgcgccgacc cgttgagtcc gggctgcatc ccttgatcac agtcaacggc
     1021 gccggcgtcc ggccgcgtag catcgcacta tgcccgcggc tttccccgcg acgaatgccg
     1081 ccttcgcacg ctccgccgcc gatccaatcg ttgccgtcgc tgcgcccgtc gtcttcgtcg
     1141 cgcgcaacct ctcgaagatc tatcgaatgg gcgaggtcga ggtgcacgcg ctgcgcgcgg
     1201 tcgatctcga aatccgcgag cgcgaattca tcgtattgct cggcccctcc ggcagcggca
     1261 agtccacgct gctcaacatc cttggcggac tggtcgttcc ttcgggcggc gaagcattgt
     1321 ggcgcgacca cgatctcgcc cacgccgacg aagcgatgct cacgcgctac cgccgagatc
     1381 acgtcggttt cgtcttccag ttctacaacc tgatccccag cctcaccgtc agcgagaacg
     1441 tcgcgctcgt gaccgacatc gtcgagaacc cgctgccggt cgacgaggcc gtcgcgcgcg
     1501 ttgggctttc cgagcgcgcg catcactttc cgtcgcagct ctccggcggc gagcagcagc
     1561 gcgtggccat cgcacgcgcc atcgccaagc ggcccgatgt cctgctctgc gacgagccga
     1621 ccggggcact cgactacgcg accggcaaga tcgtgctgga ggcgatcgcg aacatcaatc
     1681 gcgacctcgg cacgacgacg gtggtgatca cccacaacgc gccgatcgcg cagatggcgg
     1741 atcgagtgct tcgcctcgcc gatggaagaa tcgtgggcga ggatcgcaac gaacgaaagc
     1801 ggtcccctgg ggaaatctcc tggtgaaagc gctcgatcgc aagctggttc gcgacctctg
     1861 gcgcctgcgc agccaggtcg tcacggttgc gctcgtggtc gcgagcgcct tctcgggctt
     1921 cgccggatcg ctggcgacgt atctctcgct cgtccgcgcc cgggacgact tctacgcatc
     1981 cgcccgtttc gcgcacgtgt tcgtggagct gaagcgcgcg ccgctcgcgc tcatccgcga
     2041 cttcgagcgg atccccggtg tcgcggccgt acaggcgaca gtcgtgttcg acgtcgtgct
     2101 cgacgttccc aacgtcgccg aacccgtcac cggccggctg atcgggctcg ccaatggcga
     2161 cgcgtcgacg ctcgacgcgg tcgtcatccg ccgtggccgc atgccggaac cgcgttcgcc
     2221 gtccgaagtg gtcgtgtccg aaggattcgc gaccgcccgt ggtcttgcgc cgggcgcctc
     2281 gctgaaggca ctgatcaacg gcaagctgca gacgctgcag gtcgtcggca tcggattgtc
     2341 ccccgactac atctacgcga cacgaggcgg ggcattcccc gacgatcgca acttcggggt
     2401 gctgtggatg gcgcgcgaaa gcctggccgc ggcctacgac atggagggtg cgttcaacca
     2461 cctggccctg cggcttgcgc ccggagcccc cgagcgcgcg gtgctggatg cggccgaccg
     2521 attgcttcgt cgatatggcg gcacgcgtgc ctacggccgg gaccagcagg cttcgcatcg
     2581 catcctcggg caggagatcg accagtggaa ggtcatcggt gcgctgatcc cgtcgatctt
     2641 cctcgccgtg tcggcattcc tgctcaacgt cgtcctcggt cgccaggtcg cgacgcagcg
     2701 ggggcagatc gcagcattga aggcactggg ttacggcaac cgcgccctgc tgcggcatta
     2761 cctgctgcaa gtcgtggcga tcgtcaccat cggcgtcgcg atcggcgtgg ggatcggttg
     2821 gtggttcggc tccgccgtca cccgtctgta cgccgacttt ttccacttcc cgtcgtaccg
     2881 gttctcgata cccgcctggg tgctgctcgt ggccggcgcc atcaccttcg gcgccggcat
     2941 gagcggtgcc atcatcgccg tccggaagac ggtcct
//
LOCUS       DASUCJ010000751         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1047300, whole genome shotgun sequence.
ACCESSION   DASUCJ010000751 DASUCJ010000000
VERSION     DASUCJ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047300"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFU90_08990"
     CDS             <1..1116
                     /locus_tag="VFU90_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479954.1"
                     /translation="CTLESGEWDRDYIPICRWQWTPPTYGCFGMGIAHELEGMQAAIS
                     QIVRNILRSIHLFAVPRVWIDKMAKVSVHTIDNDISVNQYTGPNPPIFSTPQAASPDI
                     YQFLQWLIDLCYKQLGLSQLTAQSEKPAGLNSGVAMRTYQDVETQRFAIIGQRWERFH
                     LEEAEIILDMASRMYAKGKSIAYDVPGRGFVETIDWKDVSMRRDQYDLEAFPTSLLPR
                     TPEGQLQTVQEFLSSGLMPRDVALGQLRVPNLDAWIEDETASRDNINMCLSRIRDKGK
                     YRSPNGIANIDQCVTMAMSAWLRADIDDTMDPKRVDMLLRFLDEALAIQASKNAPPAA
                     PPGAPGAAPPPAVGQAPTPPPAPFAAAGTGSVMPAAA"
     gene            1201..2223
                     /locus_tag="VFU90_08995"
     CDS             1201..2223
                     /locus_tag="VFU90_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479955.1"
                     /translation="MAPAPVVPAVVHKTSYRTKPGTRVTVTPVGGNAAPAPNIPIQPK
                     GGAQAAAAASQPASATASPVAPSNATEGTAAAADLGAAAPPSEGTTAASPDLPEPEAF
                     RRIAAVHRAEKRFAEKQRAFAESQRTNAAQLQRVSTLENAAKAAQQNPLAAVQALGLT
                     PQQVLDAILGDAAKTPQQRQEEAMRATAQRQADIEAEIRRDRERYAQEQQTRQVTSHI
                     EANITPIFAPTDAYPHLKHAASITGEDLGQAIYREQVRVWKATGGQNGGRIPTPKEIA
                     DKAESLYAQKAALLRGKSTEPTKPATSSGTAPPPANRESAQVTKPSSPYARRDKRAYT
                     AKVVAR"
     gene            2255..3589
                     /locus_tag="VFU90_09000"
     CDS             2255..3589
                     /locus_tag="VFU90_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479956.1"
                     /translation="MSSNVNNLDAAFKQRYSAEGIARLTFEDRPFWGLVPKEEIAGAD
                     QVTRAFAVPVLFANNAAVGGNFALSQARSALVSSKVNAFQITTSKYYGFVTLDMESVE
                     RSVGNENAFVDLKSLEIDQTIENVSNRLHRNAWNNGDGTIGQVGNSTQMPSFSTSVCV
                     LNDLERMVFIQPGDELTVSPSGTPQSGERAFGSNGHGLYVIAVNLDAGTFQVGDVNGN
                     AVNLNSVTDGIPTIAALDWLAIRGDRQVPGTVGGTCFAGFQGWIPNAATGIASSGDSF
                     YNVNRYQSVDWLAGTRFDGSNFAIEDALVQGSNILAKKGGTCNAGFINHKHFSALVNS
                     ISSRGQVQFLEVSPSEYPTIGYRGVRIIGAKSDIDIIPDYACPGTVAALMNLEDWAFA
                     SVGGEDPVRFMNGDGLEFLRQPTSDSIQAYVVNYGALYCRKPRNNANITLAA"
     gene            3598..>3895
                     /locus_tag="VFU90_09005"
     CDS             3598..>3895
                     /locus_tag="VFU90_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479957.1"
                     /translation="MSGNRLYSRTLGHFEKGAVIVDAFFALDGSSNVVGYAPTTPGSA
                     VATTPYSLPKGMSNALGPAGGVLNQPHTATGTYIFTLDEPWVCLLNADVALIDQG"
BASE COUNT          750 a         1315 c         1237 g          593 t
ORIGIN      
        1 tgcacgctcg agtcgggcga atgggaccgc gactacatcc cgatctgccg atggcagtgg
       61 acgccgccga cgtacggctg tttcggtatg ggcatcgcgc acgagctcga agggatgcag
      121 gcggcgattt cgcaaatcgt ccggaacatc cttcggtcga tccacctctt cgcagtgccg
      181 cgggtctgga tcgacaagat ggccaaggtg tcggtccaca cgatcgacaa cgacatttcg
      241 gtcaatcagt acaccggacc gaatccgccc atcttctcga cgccgcaagc ggcgagtccg
      301 gacatctacc agttcctcca gtggctcatc gacctctgct acaagcaact agggctctcg
      361 cagctcacgg cgcagagcga gaagccggcg ggcctgaaca gcggcgtcgc gatgcgcacg
      421 taccaggacg tcgagacgca gcggttcgcc atcatcggcc agcggtggga acggttccac
      481 ctagaagagg cggagatcat cctcgacatg gcctcgcgta tgtacgcgaa agggaaatcg
      541 atcgcgtacg acgtcccggg gcgcggcttc gtcgagacca tcgactggaa agacgtctcg
      601 atgcgacgcg accagtacga tctggaagcg tttcccacgt cgctcctgcc gcgtacgccc
      661 gaaggtcagc tgcaaacggt gcaggagttc ttgtcgtcgg gcctcatgcc tcgcgacgtg
      721 gcgctcggcc agctccgtgt gccgaacctc gacgcgtgga tcgaggacga gacggcgagt
      781 cgcgacaaca tcaacatgtg cctctcgcgc atccgcgaca agggcaagta ccgttcgccg
      841 aacggcatcg cgaacatcga ccagtgcgtg acgatggcga tgagcgcgtg gctccgcgcg
      901 gacatcgacg acacgatgga tcccaagcgc gtggacatgt tgctccgctt cctcgacgag
      961 gcgctggcga tccaggcctc gaagaacgcg cctcccgccg cgccgcccgg cgctcccggc
     1021 gccgccccgc ctcccgccgt cgggcaagcc ccgacgccgc cgcctgcacc ctttgccgcc
     1081 gccggaacgg gctccgtgat gcccgccgcg gcgtaacccg gagacctcct catgaccgat
     1141 gttgctgccc ccgttcccgc cgccgttgct actcccgtcg cgccttcggg cgcgagtccc
     1201 gtggcgccgg cccccgtcgt accggcggtc gtccacaaga cgtcgtaccg gaccaagccg
     1261 ggcacgcgcg tgactgtcac gccggtcgga ggcaacgcgg ctcccgcgcc gaacatcccg
     1321 atccagccga agggcggcgc gcaagcggct gccgcggcgt ctcaacccgc ctccgcaacc
     1381 gcgtctcccg ttgcgccgtc gaacgcgaca gaagggacgg ccgcggcggc ggatttaggc
     1441 gccgcggctc cgccctcgga aggcacgacg gcggcgagcc cagaccttcc cgagccggaa
     1501 gcgttccggc ggatcgccgc ggtgcatcgc gcggagaaaa ggttcgccga aaagcagcgc
     1561 gctttcgccg agagtcagcg cacgaatgcg gcgcagctgc agcgcgtctc gacgctcgaa
     1621 aacgccgcga aggcagcgca gcaaaacccg ctcgcggccg tccaggcgct cggccttacg
     1681 ccgcagcagg tgctcgacgc catcctcgga gacgccgcca agacgccgca gcagcgccag
     1741 gaggaggcca tgcgcgcgac ggcgcagcgg caggcggaca tcgaagctga gattcgcagg
     1801 gaccgggaac ggtatgccca agagcagcaa acccgtcagg tgacgtccca catcgaagcg
     1861 aacatcaccc ccatcttcgc tccaacggac gcgtatccgc atctcaagca tgcggcgagc
     1921 atcaccggtg aagatttggg acaggcgatc taccgggagc aggtgcgagt ctggaaggcg
     1981 actggtggac agaacggagg acgcattccg actccgaaag agattgcgga caaggcggag
     2041 tcgctctacg ctcagaaagc agcacttctt cgcgggaaat ccaccgagcc cacgaagcca
     2101 gctacgtcga gcgggaccgc gccaccgcca gccaatcgag agagcgccca ggtgaccaag
     2161 ccgagcagtc cctacgcacg tcgcgataag cgcgcgtaca cggccaaggt ggtcgcacgt
     2221 tagaggctgt tcggcccacc caagggcctg aacgatgtcc tcgaacgtca acaatctcga
     2281 cgcggcattc aagcaacggt actcggcaga aggaattgcg cgactcacct tcgaagatcg
     2341 ccccttttgg ggtctggtcc cgaaggagga aatcgcgggc gcagatcagg tgacccgcgc
     2401 gtttgcggtg cctgtcctct tcgcaaacaa cgcggccgtc ggcggcaact tcgcactctc
     2461 gcaggcgcgg agcgcgctcg tgtcctcgaa ggtcaacgcg tttcagatca cgacgagcaa
     2521 gtactacggc ttcgtcacgc tcgacatgga atcggtcgag cgatcggtcg gaaacgaaaa
     2581 tgcgttcgtc gacctgaagt cgctcgaaat cgaccagacg atcgagaacg tctcgaatcg
     2641 cctgcaccgc aacgcctgga acaacggcga cggcacgatc ggccaggtcg gcaactcgac
     2701 gcagatgccg tcgttctcga cgtcggtttg cgttctgaac gacctcgaac gcatggtgtt
     2761 cattcagccg ggcgacgagc tcacggtctc gccgagcggc actccgcagt cgggcgagcg
     2821 agccttcggc tcgaacggac acggcctcta cgtcatcgcc gtcaacctcg acgcgggaac
     2881 gttccaggtg ggcgacgtca acggcaacgc cgtgaacctg aacagcgtga cggacggtat
     2941 tccgaccatc gccgcgctcg actggctcgc cattcgcggc gaccgccagg tgccggggac
     3001 cgtcggcgga acgtgcttcg ccggcttcca ggggtggatt ccgaatgccg cgacgggcat
     3061 cgcgagctcg ggcgattcct tctacaacgt gaaccggtac cagtcggtcg actggctcgc
     3121 cggaacgcgg ttcgatggct cgaacttcgc catcgaggac gcgctggtgc aggggtcaaa
     3181 catcctcgcg aagaagggtg gcacctgcaa cgcgggcttc atcaatcaca agcacttctc
     3241 ggcgctcgtg aacagcatca gctcgcgagg gcaagtgcag ttcctcgagg tctcgccgag
     3301 cgagtacccg acgatcggct atcgaggcgt gcgtatcatc ggcgccaaga gcgacatcga
     3361 cattatcccg gactacgcgt gccccggcac ggtcgccgcg ctgatgaacc tcgaggattg
     3421 ggcgttcgcg tccgtcggcg gggaggaccc agtgcgattc atgaacgggg acgggctcga
     3481 gttcttgcgt cagccgacct cggactcgat ccaggcgtac gtcgtcaact acggcgccct
     3541 ctactgccgc aagccgcgca acaacgcgaa catcacgctc gcggcgtgag gtgacccatg
     3601 agcggcaatc gcctttattc gcgcacgctg ggccacttcg aaaaaggggc cgtcatcgtc
     3661 gacgcgttct tcgcgctcga cggatcgagc aacgtcgtgg gctacgctcc gacgacgcct
     3721 ggaagcgccg tcgcgacgac gccgtactcg ctgccgaagg gtatgagcaa cgcgctcggc
     3781 cccgccggtg gcgttctgaa tcagccccat accgcgacgg gcacgtacat cttcacgctc
     3841 gacgagccat gggtgtgtct gctgaacgca gacgtcgccc tcatcgatca gggcg
//
LOCUS       DASUCJ010000752         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1490440, whole genome shotgun sequence.
ACCESSION   DASUCJ010000752 DASUCJ010000000
VERSION     DASUCJ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490440"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VFU90_09010"
     CDS             <1..1377
                     /locus_tag="VFU90_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479958.1"
                     /translation="EATVASNTWRTADLANQSLVFAFAEPLGLQSPAPQPSGMRGYTP
                     VLMAGTHTTAAPAIVVPVPSAGPGLGAAAGAASQAAAAFGNATPSPTPSAAPAASGPI
                     PVALQLDVTVAAPNVANVTVTRPIFDRISAADRAAGRAATATLAPIAIYPFGTAWSVA
                     VNFGTGVAGGGDRKPVDPNADDPQAIGHALGAMQRAYYVTRRAVFADALGGARTPVIG
                     AHPSVTFVGLTPYTAGPVPYGLAVDRANEGAAPDGGNAAASLAWGVASVYAERLAVAA
                     PAMMRKSDALDQLPFDDALEMFYLARNTNVPPAMVHSSSDVAALTAPDDAKVRLTASI
                     AGGKSAVAPSKPVSYGGSNDFGWWILGSDGSVSDEMQNGMHAELEGEGIILENDAEAA
                     ITYRRNGFIVRCVGMAAGALMALGVTISPGGEGGQEIAEQAAEQINAIREANEAEEMR
                     EDALKCAE"
     gene            complement(1385..1705)
                     /locus_tag="VFU90_09015"
     CDS             complement(1385..1705)
                     /locus_tag="VFU90_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479959.1"
                     /translation="MEQPQQPKSLSLDVWSVTAASALTALIVAILSLPLHLMHAGRMV
                     GGAMGGPRAGMPMQGMMQPHLAPSPGMILVWVLIVLLVVFVYGGIAGAIFAALYNAIL
                     TRRP"
     gene            complement(1805..2263)
                     /locus_tag="VFU90_09020"
     CDS             complement(1805..2263)
                     /locus_tag="VFU90_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5479960.1"
                     /translation="MAVVLDASATLAWLFDDERDDGAIAMAEAILRDGAIVPALWRLE
                     LRNALLAAERRGRLTAKEVAEILADVRTLPIEAAQALSRSSSESELELARTHRLSVYD
                     AAHLELAVRLGVPLMSRDASLNAAAADLGLRWQPPKRRTSRRSRRRTVSS"
     gene            complement(2568..>2775)
                     /locus_tag="VFU90_09025"
     CDS             complement(2568..>2775)
                     /locus_tag="VFU90_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479961.1"
                     /translation="ASGGGLDALLSLLWQCLAVHGFLLLSSWAVFLAASPGVRFLIVA
                     GWVSYIVAAAAFVASVIRDARRSG"
BASE COUNT          494 a          891 c          979 g          411 t
ORIGIN      
        1 gaagcgacgg tggcaagcaa tacgtggcgc accgccgatc tcgcgaacca atcgctggtg
       61 tttgcattcg ccgagccgct cggactgcag tcgcccgcgc cgcagccgag cggaatgcgc
      121 ggctacacgc cggtgctgat ggccggcacg cacacgacgg cggcgccggc tatcgtggtg
      181 ccggtgccgt cggcggggcc gggtttgggc gcggcggcgg gcgcagcttc gcaagcagcg
      241 gcggcgttcg gcaatgcgac gccgtcgccc acgccgagcg cggcgccggc cgcgagcgga
      301 ccgattccgg tggcgttgca gctcgacgtc acggtggccg caccgaacgt tgcaaacgtt
      361 acggtgacgc ggccgatctt cgatcgcatt tccgcggccg accgcgcggc gggacgtgcc
      421 gcgaccgcaa cgcttgcgcc gatcgcgatc tatccgttcg gtacagcgtg gagcgtggcg
      481 gtgaatttcg ggaccggcgt agcgggcggg ggcgaccgca agccggtcga tccgaacgcc
      541 gacgacccgc aggcgatcgg gcacgcgctc ggcgcgatgc agcgcgcgta ttacgtgacg
      601 cgccgcgcgg tgttcgccga cgcgctcggc ggagcgcgca cgccggtgat cggcgcgcat
      661 ccaagcgtga cgttcgtcgg gctaacgccg tatacggccg ggccggttcc gtacgggttg
      721 gcggtcgacc gcgccaacga aggcgccgcg ccggacggcg gcaatgcggc cgcgagtttg
      781 gcgtggggtg tcgcatcggt gtacgccgag cggctggcgg tcgcagcgcc ggcaatgatg
      841 cgcaagagcg acgcgctcga tcagctgccg ttcgacgacg cgctcgagat gttttacctc
      901 gcgcgcaata ccaacgttcc gccggcgatg gtgcattcga gttccgacgt ggcggcgctg
      961 acggcgccgg acgacgccaa ggtgcgcttg acggcgagca tcgcgggcgg taagagcgcg
     1021 gtcgcaccgt cgaaaccggt gagctacggc ggatcgaacg acttcggctg gtggattctc
     1081 ggcagcgacg gatcggtgag cgacgagatg caaaacggga tgcacgccga gctggaaggc
     1141 gaggggatca tcctggaaaa tgacgccgag gccgccatca cctaccggcg caacggcttc
     1201 atcgtgcgat gcgtcggaat ggctgccggc gcgttgatgg cgctgggcgt cacgatcagt
     1261 ccgggcggcg aaggcggtca ggaaatcgcc gagcaggccg ccgagcagat caacgcgatt
     1321 cgggaagcca acgaagccga agagatgcgc gaagacgcgc tcaagtgcgc cgagtagcgc
     1381 gctgctaggg tcgccgggtg agaatggcgt tgtagagcgc ggcaaagatc gcgccggcaa
     1441 tgccgccgta cacgaacacg acgagcagta cgatcagcac ccagacgagg atcatccccg
     1501 ggctcggcgc caggtgcggc tgcatcattc cttgcatcgg catcccggcg cgcggaccgc
     1561 ccatcgctcc accgaccatc cggcccgcgt gcatgagatg cagcggcagc gataagatcg
     1621 ccacgattag cgccgtgagc gcggacgcag cggtcaccga ccagacgtcg agcgacaagg
     1681 atttcggttg ttgcggctgt tccatggtaa gccccctttc cgcgtattct acagcctatc
     1741 gctgaacgcg ggctaagtat aaaatagaaa gccgtccttc gactgcggcg ctgcgcgcct
     1801 ccgctcagga tgacacggtg cggcggcggg aacggcgcga ggtgcgcctc ttcggcggtt
     1861 gccagcgcag ccccaaatcg gcggccgcgg cgttgagcga ggcgtcgcgg ctcatgagcg
     1921 gaacgcctaa gcgaacggcg agttctagat gggccgcgtc gtacacgctc aggcggtgcg
     1981 tgcgggcaag ctcgagttcc gactccgacg acgatcgcga taacgcttgg gccgcttcga
     2041 tcggcagggt gcggacgtcg gcgagaatct cggcaacctc tttcgcggtc aggcgtccgc
     2101 gacgctcggc ggcgagcagc gcgttgcgca gctccagccg ccagagcgcg ggcacaattg
     2161 caccgtctcg aagaatcgct tcggccatgg cgatagcccc gtcgtcgcgt tcatcgtcga
     2221 acagccaggc cagcgttgcc gacgcgtcga ggacaaccgc cacctagcga cgccgcccct
     2281 cttcgatcag ggtgcgggcg cgaatcccga gccgtatcgt acgcgcaagg atccggtcta
     2341 gcgccgcgcg cgcggcttcc ttttccgggt cgccgagcgg acccagctga gcgacgggct
     2401 ttccatttcg agtaatcaca atcgtttcgc cgtgcgcggc ggcgtcgacg agagccgaga
     2461 agtgcgtctt accttcgaac gtccctacct tacgcatcta actagtctaa cagactagtt
     2521 agagagctgc aagctcgagg ttgaaatgct gcgtatcgga tggcggttta gccggacctt
     2581 cgtgcatccc gaatcacgct tgcaacgaac gcggcggccg cgacgatgta gctgacccag
     2641 ccggcgacga tgaggaatcg tacgccgggc gacgcggcga gaaacaccgc ccacgagctc
     2701 agcagcaaga agccgtgaac cgcgagacac tgccacagca gggaaagcag ggcatcgagc
     2761 ccgccgccgg acgcg
//
LOCUS       DASUCJ010000753         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_344367, whole genome shotgun sequence.
ACCESSION   DASUCJ010000753 DASUCJ010000000
VERSION     DASUCJ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344367"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="pnp"
                     /locus_tag="VFU90_09030"
     CDS             <1..1182
                     /gene="pnp"
                     /locus_tag="VFU90_09030"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5479962.1"
                     /translation="QRLDGIVALEDKRYMHFYNFPPYSVGETRPMRGPGRREIGHGAL
                     AERALLPVLPPKEEFPYTIRVMSEVLESNGSSSMASVCGSTLALMDAGVPIREHVAGV
                     AMGLILKGDKYAVLTDIQGLEDALGEMDFKVAGTKKGITAIQMDIKVQGITLKIMREA
                     MEEARKSRYFIIDKLAEAIAQPRTELSKYAPRMIVIKIDPAKIKDVIGPGGKVINKII
                     ADTGVTKIDIEDDGSVFITSLDGESGDKARQIVENLTKDIVVGETYTGTVTRIIPIGA
                     FVQVLPGKEGLVHISQLATQRVEHVEDVVKVGDDLTVKVVEIDGQGRLNLSHKAVLTG
                     GNGSGGPGPGGGRGSWEDGDRNRRRPRDTSETRDAAPAADAPGAPPMRRRRRPHGRRD
                     D"
     gene            complement(1183..>1880)
                     /locus_tag="VFU90_09035"
     CDS             complement(1183..>1880)
                     /locus_tag="VFU90_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479963.1"
                     /translation="GIDATGPGFEVTAGAGLFGVGYTGGWGGTSLPSFGHLATPSLTL
                     RLFPDSRWSANLGASGSFTLPSLWQQYGLPDSYGQLVYDRNSLYDATLSYTDEARVRV
                     SVEAASQRVSGYTNGLVTSTGASFSWQVAPTIALRAWTMYVADSTAPPAAVPYYPAGM
                     PSNVNAFWLTYENGSAVRIDAIYRRDVLNAQPFEHLDAAISGPLTEHVRWYAGVEDRL
                     RTTYLDIGVRLTR"
BASE COUNT          381 a          600 c          624 g          275 t
ORIGIN      
        1 cagcgcctcg acggcatcgt cgcgctcgaa gacaagcgct acatgcactt ctacaacttc
       61 ccgccgtatt cggtcggcga aacgcgcccg atgcgcggcc cgggacgccg cgagatcggg
      121 cacggcgcgc tcgccgagcg cgcgcttctg ccggtgctcc cgccgaaaga agagtttccc
      181 tacacgatcc gggtgatgag cgaagtactc gaatcgaacg gctcgtcgtc gatggcgtcg
      241 gtctgcggat cgacgctggc gctgatggac gccggcgtac cgattcgcga acacgtcgcc
      301 ggagtcgcca tggggctgat cctcaagggc gacaaatacg ccgttctcac cgacatccag
      361 ggcctcgaag acgcgctggg cgagatggac ttcaaagtcg ccgggacgaa gaaaggcatc
      421 accgcgatcc agatggacat caaggtccag gggatcacgc tgaagatcat gcgcgaagcg
      481 atggaagaag cgcgcaagtc gcggtatttc atcatcgaca aactggccga agcgatcgcg
      541 cagccgcgaa ccgaactctc gaagtacgcg ccgcggatga tcgtcatcaa gatcgatccg
      601 gcgaagatca aggacgtgat cggccccggc ggtaaggtga tcaacaagat catcgccgat
      661 accggcgtta ccaagatcga catcgaggac gacggttcgg tgttcatcac ctcgctcgac
      721 ggcgaatccg gcgacaaggc gcggcagatc gtcgagaacc tcaccaaaga catcgtagtc
      781 ggcgagacct atacgggcac cgtcacgcgc atcattccga tcggcgcgtt cgtgcaagtg
      841 cttccgggca aggaaggctt ggtgcacatc agccagctgg ccacgcaacg cgtcgagcac
      901 gtcgaagacg tcgtgaaagt cggcgacgac cttacggtaa aggtcgtcga gatcgacggt
      961 cagggccgct tgaatctctc gcacaaagcg gtactcaccg gcggcaacgg cagcggcggc
     1021 cccggccccg gcggcggccg aggttcgtgg gaagacgggg accgcaatcg ccggcggccg
     1081 cgcgatacgt ccgagacgcg cgacgcggcg cctgccgccg acgctccggg cgcaccgccg
     1141 atgcggcggc gtcgccggcc gcacgggcgg cgcgacgact agctagcggg ttagccgaac
     1201 tccgatatcg agatacgtgg tgcgcaggcg gtcttcgacc cctgcgtacc aacgtacgtg
     1261 ttcggtcagc ggccccgaaa tcgccgcgtc gagatgctcg aagggttgcg cgttgaggac
     1321 gtcgcgccgg tagatcgcat cgatccgtac cgccgagccg ttctcgtacg tcagccagaa
     1381 ggcgttcacg ttcgagggca taccggccgg gtagtaagga accgccgccg gaggcgcggt
     1441 cgaatcggcg acgtacatcg tccacgcgcg cagcgcgatc gtgggcgcga cctgccagga
     1501 aaacgaagcg ccggtcgaag tgacgagccc gttggtgtag ccgcttacgc gctgcgacgc
     1561 ggcttcgacg ctgacgcgca cgcgagcttc gtcggtatac gacagggtcg catcgtagag
     1621 cgaattgcga tcgtagacga gttgcccgta gctgtcaggc aggccgtact gctgccaaag
     1681 cgacggcaac gtgaacgaac cgctcgcgcc gaggttcgcg ctccagcgcg agtccggaaa
     1741 aagccgtagc gttaacgacg gcgtcgccaa atggccgaac gacggcaacg acgtaccgcc
     1801 ccagccgccg gtgtaaccga ccccgaagag tcccgctccg gccgtgactt cgaagcccgg
     1861 gccggtcgcg tcgatgccgg
//
LOCUS       DASUCJ010000754         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1291770, whole genome shotgun sequence.
ACCESSION   DASUCJ010000754 DASUCJ010000000
VERSION     DASUCJ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291770"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..>1149
                     /locus_tag="VFU90_09040"
     CDS             41..>1149
                     /locus_tag="VFU90_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5479964.1"
                     /translation="MTSRLASSLARLGVKRGDRVFLRLPNIPEFYVAALAVAKLGGVF
                     VPSSTQFRASEVAYRLKDSGAVAAITTSGLVDVIDEAAASIRELIQIIVVPYPEPMTL
                     GDQHIEFGKLVSEGSEAFVPAQTRNDDIAFIAYTSGTTGDPKGVVHYHRYPIAYEGLV
                     RYWHDYRPDDVVACPSELGWLLPIACTFLYALSRGLTVVLYDAMGDKFDAERWFALFQ
                     KYRITNFTAPPTTYRMMMAAAPAANRFDMSSWRHAVSAGEPLPANTLEAIQRQFGITP
                     IDGIGMTECMVYCFNRIGMPLKPGSCGRPGPGTVIELMDEDMRPVPVGAEGVLCLRRD
                     THPGMMKEYWNKPERTAEIFRGEWYYSGDVLARDE"
BASE COUNT          203 a          352 c          398 g          196 t
ORIGIN      
        1 gcatgcgagc cggtcgttgg agtactcgga actcgacgcg gtgacgagcc gactggcatc
       61 gtcgctggcg cggctgggcg tgaaacgcgg cgaccgcgtg ttcttgcgcc tgccgaacat
      121 cccggagttc tacgtcgccg cgttggcggt cgctaagttg ggcggcgtgt tcgttccgtc
      181 gagtacgcag ttccgagcga gcgaggtcgc gtaccggctg aaggattcgg gcgcggtggc
      241 ggcgatcacg acgagcgggc tggtggacgt gatcgacgag gcggcggcga gcatccggga
      301 gttgatccag attattgtcg tcccgtaccc ggaacccatg acactcggcg accagcacat
      361 cgagttcggc aagctggtca gcgaggggag cgaggcgttc gtcccagcac agactcggaa
      421 cgacgacatc gcgttcatcg cctacacctc gggcacgacc ggcgacccga aaggcgtggt
      481 tcactaccac cgttacccga tcgcttacga agggctggtg cggtactggc acgactaccg
      541 gccggatgac gtggtcgcgt gcccgtcgga actcggttgg ctgctaccaa tcgcgtgtac
      601 gttcctgtac gccttgtcgc gcgggctgac ggtcgtgctc tacgacgcga tgggcgacaa
      661 gttcgacgcg gagcgatggt tcgcgctgtt tcagaagtat cgcatcacga acttcacggc
      721 gccgccgacg acctaccgca tgatgatggc cgcagccccc gcagcgaatc gattcgacat
      781 gtccagctgg cggcacgcgg tcagcgcggg cgagccgctg ccggccaaca cgctggaagc
      841 gatccagcgt cagttcggta ttacaccgat agacggcatc ggcatgaccg agtgcatggt
      901 gtactgcttc aatcgcatcg gcatgccgct caagcccgga agctgcggcc ggcccggccc
      961 gggcacggtg atcgagctga tggacgagga catgcggccg gtgccggtcg gggctgaggg
     1021 tgtactctgt ctccgccgcg acacgcatcc gggcatgatg aaggaatact ggaacaagcc
     1081 cgagcgcacc gcggagatct tccgcggcga gtggtactac tccggcgacg tgctcgcacg
     1141 cgacgagga
//
LOCUS       DASUCJ010000755         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1306959, whole genome shotgun sequence.
ACCESSION   DASUCJ010000755 DASUCJ010000000
VERSION     DASUCJ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306959"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VFU90_09045"
     CDS             complement(<1..1117)
                     /locus_tag="VFU90_09045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5479965.1"
                     /translation="MTTVTETTTSAIARIRLRAASAEPWVAALVVLGLALRLWWLFTH
                     TNAIEKDGAEYARIAQNVLHGHGYVGLQPGPELLFPPLYPALLAGLSPIFGIEHAGRV
                     ISLLLGSALPIPAYLVGRRLFGRRTGIAAAAFIAFNPILIAYSTAVLSETTYLTLQLT
                     AAYLLLRAHDRRDLIRAAVAGACWTLAALVRPEAALILGLFTFAAVVVGLIYRSQRAA
                     MKVAVMALAVCAATFLPYAAWTSHQAGRLQWSGKATVSSFINQRIDDGMSYTVAISAV
                     DADGRPRGVLLDPNSYIKGDYGKAPLFSPSAELKFERSGVHHLWSNFGRNAVLGAPLL
                     ALLAAVGLLGGPWTRRHNENMLLLIVYGGANAVIFGAI"
BASE COUNT          224 a          346 c          389 g          175 t
ORIGIN      
        1 cgatggcgcc gaagatgacc gcgtttgcgc cgccgtacac gatcaacagc aacatgtttt
       61 cgttgtgtcg tcgggtccaa ggtccgccta agagaccgac ggcggcgagt aacgcgagca
      121 acggggcgcc aagcacggcg ttgcgcccaa aattcgacca aaggtggtgg acaccggagc
      181 gttcgaactt gagctcggcg ctcggcgaga acaacggcgc ttttccgtaa tcgcctttga
      241 tgtaagagtt ggggtcgagc aagacacccc tcgggcgacc gtcagcgtca accgccgaga
      301 tcgcaacggt gtaagacatg ccgtcgtcga tgcgttggtt gatgaacgac gacacggtcg
      361 ctttcccgct ccactgcaag cgccccgcct gatgcgacgt ccaagcggcg tagggcagaa
      421 acgtcgcggc gcagaccgcc agcgccatca cggcgacctt catcgccgcc cgttggctgc
      481 ggtaaatgag gccgacgacc acggcggcaa atgtgaaaag cccgaggatt agcgcggcct
      541 ctggtcgcac gagcgccgct agcgtccagc acgcgcccgc cacggccgct cggatgaggt
      601 cgcgccgatc gtgtgctcgc agcagcaggt acgcagcggt gagctgcagc gttagatagg
      661 tcgtctccga gagcacggcc gtcgaatacg cgatgagaat ggggttgaac gcgataaagg
      721 cggcggccgc gattccggtg cggcggccaa acagccgtcg gccgacgagg taggcgggta
      781 tcgggagagc gctcccgagc agcagcgaaa tcacccggcc ggcgtgctcg atgccgaaaa
      841 tcggcgaaag accggcgagg agagctggat acagcggtgg gaacaacagc tcggggcccg
      901 gctgcaatcc gacgtatccg tggccgtgca acacgttttg cgcgatgcgc gcgtactcgg
      961 cgccgtcttt ctcgatcgcg ttggtgtggg tgaaaagcca ccaaaggcga agggcgagcc
     1021 cgagcacgac gagcgcggcc acccacggct cggcgctcgc ggcgcgcagc cttattcggg
     1081 caatcgcact ggtggtggtc tcggtgaccg tcgtcacggc gtgttgcata tgcg
//
LOCUS       DASUCJ010000756         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1078001, whole genome shotgun sequence.
ACCESSION   DASUCJ010000756 DASUCJ010000000
VERSION     DASUCJ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078001"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFU90_09050"
     CDS             complement(<1..1026)
                     /locus_tag="VFU90_09050"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5479966.1"
                     /translation="MPLRFRAALLRASLLVACAPSIAHAQQFDSTMFAGLQWRMIGPY
                     RGGRTVASTGVRGQPNVFYAAATDGGVWKSTDYGRVWTPIFDREPVGSIGALAVAPSD
                     PAIIYVGSGEGLQRPDLSIGDGMYKSTDGGRAWTHLAGLRGGQQTAAILVDPRDADRL
                     FVAVLGHPYGPNVERGVYRSTDGGATFERVLFKDENTGAVALAFDPGNAQVVYAVLWA
                     ARQGPWEYDNAYAGPGSGLYKSVDGGATWTQLTTGLPTPAQGLGRIGIAVAPSDGRRL
                     YAMVQAPPKFGGVYRSDDAGATWTRVNGEERVYGRGDDFAEVQVDPTNPDEIYVANTS
                     TYRSTDGG"
     gene            1157..1597
                     /locus_tag="VFU90_09055"
     CDS             1157..1597
                     /locus_tag="VFU90_09055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479967.1"
                     /translation="MNVRPARTADASSIKALIDLYVSDGTLLPRTLDFIREHIADFLV
                     ATDDEVVVGCVHVEEYAPSLAEIRSLAVDPGAQGQGIGAALVRTAEELAQRRELATLF
                     AVSNSEQFFRGLGYEPEHVPELDRERSEVSKYKGVYARALPRRR"
BASE COUNT          254 a          514 c          590 g          251 t
ORIGIN      
        1 gccgccatcg gtcgagcgat acgtcgaggt gttcgcgacg tagatctcat cggggttggt
       61 cggatcgacc tggacttcgg cgaagtcgtc gccgcggccg tagacgcgct cttcgccgtt
      121 cacacgggtc cacgtcgcgc cggcgtcgtc ggagcggtac acgccgccga acttcggcgg
      181 cgcctgcacc atcgcgtaca gcctccggcc atcgctcggc gccacggcga tgccgatgcg
      241 gccgaggccc tgcgcgggcg tcgggagacc ggtggtgagc tgggtccacg tcgcgccgcc
      301 gtcgaccgac ttgtacaatc cgctgccggg cccggcgtac gcgttgtcgt actcccacgg
      361 cccctggcgc gcggcccaga ggaccgcgta caccacctgc gcgttgcccg ggtcgaacgc
      421 cagcgccacc gcgcccgtgt tctcatcctt gaacagcacg cgctcgaacg tggcgccgcc
      481 gtcggtcgag cgatagacgc cgcgctctac gttaggcccg tacggatgac cgagcaccgc
      541 gacgaagagg cggtcggcat cgcgcgggtc cacgagaatc gcggccgtct gctggccgcc
      601 gcggaggccg gccagatgcg tccacgcgcg gccgccgtcc gtcgacttgt acatcccgtc
      661 gccgatcgac aggtcgggcc gttgcaaccc ttcgccgctg cctacataaa tgatggcggg
      721 atcggatggc gccacggcga gcgcgccgat cgatccgacc ggttcacgat cgaagatcgg
      781 cgtccacaca cggccatagt cggtgctctt ccagacgccg ccatcggttg cggccgcgta
      841 gaacacgttg ggttgtccgc ggacgcctgt cgaggccacg gtgcggccgc cgcgatacgg
      901 gccgatcatg cgccattgga gaccggcgaa catcgtcgaa tcgaattgtt gggcgtgcgc
      961 aatcgatgga gcgcaggcaa cgaggagcga tgcgcggagc agtgcggcgc ggaagcgcaa
     1021 tggcatggag gatcggggcg cggtcacggg atggagacgg gcgagcagat agcagacaac
     1081 gcgggcgcgt cgggcgcaag atctacgcgg cgcgatgggc tcgcggcatc gttgatcacc
     1141 actgaaggag cgaagcatga acgtacgtcc cgctcgaacc gcggatgcgt cgtcgatcaa
     1201 ggcgttgatc gatctctatg tgtcggatgg cacgctgctg ccgcgcacgc tggacttcat
     1261 tcgcgagcac atcgccgact ttctggtcgc gacggacgat gaggtggtcg tgggctgcgt
     1321 gcacgtggag gagtacgcgc cgagtctggc agagatccgg tcgttggcgg tggatcccgg
     1381 cgcgcagggg cagggcatcg gcgccgcgct ggttcggacg gccgaggagt tggcgcagcg
     1441 gcgggagctt gcgacgttgt tcgcggtgag caacagcgag caattctttc gcgggttggg
     1501 ctacgagccc gagcacgtgc cggagctgga ccgggagcgg agcgaggtga gcaagtacaa
     1561 gggcgtctac gcgcgggcgt tgccgcgccg gcggtagtgc cgcgtcgct
//
LOCUS       DASUCJ010000757         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_191246, whole genome shotgun sequence.
ACCESSION   DASUCJ010000757 DASUCJ010000000
VERSION     DASUCJ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191246"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU90_09060"
     CDS             <1..184
                     /locus_tag="VFU90_09060"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5479968.1"
                     /translation="EALQRMFSMESAGTARDAALLDEIGRAMRDASICGLGQTAANAI
                     ASARTVLPGFSANGDV"
     gene            181..1080
                     /locus_tag="VFU90_09065"
     CDS             181..1080
                     /locus_tag="VFU90_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5479969.1"
                     /translation="MIRGPIPLDPPQPAKPAIELNVDGKPVTVAQGATILDACSAAGV
                     QTPTLCFLQTLTPVNACRVCVVEVEGARVLVPACARQAEAGMSVRTDTERVRHSRKMV
                     LEFLASSVDLSTAPDALRYCERYGADPTRYGADAATVKQPPKIDNNLYVRDYSKCILC
                     YKCVEACGTDAQNTFAIAAAGRGFDAHIATEFDVPLPDSACVYCGNCIGVCPTGALMF
                     VSEHEMRAAGTWDESRQTQTDTICPYCGVGCTLRMQVQDNEIVKVSSPLENGVTHGSL
                     CIKGRFGTVYVQNREPDRSRPPR"
     gene            1077..1946
                     /gene="fdhD"
                     /locus_tag="VFU90_09070"
     CDS             1077..1946
                     /gene="fdhD"
                     /locus_tag="VFU90_09070"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU5479970.1"
                     /translation="MTRAADAPNVRPGRCIDLEVLALDGAAGSLKNDRVVTEEPLELR
                     LVAGKNAQTLAVTMRTPGNDFELAAGFAFSEGIVTAREELAGLSYCLDPGVDPDQRYN
                     IVNVELARRDLPDLERFERHFTVGSACGVCGRTQLDSLRDRGVKPIEETMTVATGLLY
                     SLPGRMREAQRIFASTGGLHAAALFDESGEAVVVREDIGRHNAVDKLVGWGLLNGRLP
                     FTRSVLMVSGRAGYEILQKSAVARIPVVCSVSAPSSLAVDLAREFEITLAGFVRDDRA
                     NVYSRADRIAAAR"
     gene            complement(1966..>2559)
                     /locus_tag="VFU90_09075"
     CDS             complement(1966..>2559)
                     /locus_tag="VFU90_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5479971.1"
                     /translation="PDPDPSLITRAGFAADVLAVADAAGAGRFTLVGCSLGGVVAFEL
                     WKLAPERFDAMAIVGSFAYYPNARAYADGVGAIVREAGGMRAFAEARAAKLGLPPARL
                     AETIEQMACKSVPCYLASTEATWTGDYRELLPAIAVPTLVLCGERDAIAPYPLSEEIA
                     RGIPGARLAIVEGAGHVANADNPGRFNELLGDFLGSA"
BASE COUNT          427 a          878 c          860 g          394 t
ORIGIN      
        1 agaagcgctg caacgcatgt tctcaatgga gtcggccggc acggcacgcg acgcggcgct
       61 gctcgacgag atcgggcgcg cgatgcgcga cgcgtcgatt tgcggtttgg ggcagaccgc
      121 cgccaacgcg atcgcatccg cccgaacggt ccttcccggt ttttccgcga acggcgacgt
      181 atgatccgcg gcccgatccc cctcgacccg ccgcaaccgg ccaaacccgc gatcgagctg
      241 aacgtcgacg gaaagccggt taccgtcgcg caaggcgcga cgattctcga cgcttgcagc
      301 gccgccggcg tgcaaacccc gacgctgtgc tttctgcaga cgctcacccc ggtcaacgcc
      361 tgtcgcgtct gcgtggtcga agtcgagggc gcgcgcgtgc tcgttccggc ctgcgcgcgc
      421 caggccgaag cggggatgag cgtgcgtacc gacaccgagc gcgtccgcca cagccgcaag
      481 atggtgctgg agttcctcgc ttcgtcggtc gatctctcga ccgcccccga cgccctgcgt
      541 tactgcgagc gttacggcgc cgacccaacc cgatacggag cggacgccgc gaccgtgaag
      601 cagccgccga agatcgacaa caatctctac gtccgcgact actccaagtg catcctgtgc
      661 tataagtgcg tcgaggcctg cgggaccgat gcccagaaca cgtttgcgat cgccgccgcg
      721 ggacgcgggt tcgacgcgca catcgcgacc gagttcgacg ttccgctgcc cgactcggcg
      781 tgcgtgtact gcggcaactg catcggcgtc tgtccgaccg gcgcgctgat gttcgtctcc
      841 gagcacgaaa tgcgtgcggc gggaacgtgg gacgaatcgc ggcaaacgca aaccgacacg
      901 atctgcccgt actgcggtgt cggctgcacg ctgcgcatgc aagtgcagga caacgagatc
      961 gtcaaggtct cgtcgccgct cgagaacggc gtcacgcacg gcagcctctg catcaaaggg
     1021 cggttcggca cggtgtacgt gcagaatcgc gagccggatc gcagccggcc gccgcgatga
     1081 cgcgcgcggc ggacgccccc aacgtacggc cgggacgctg catcgatctc gaagtactcg
     1141 cgctcgacgg cgccgcgggc tcgctcaaaa acgatcgcgt ggtcaccgaa gaaccgctcg
     1201 aactgcggct ggtcgccggt aaaaacgcac aaaccctggc ggtgacgatg cgcacgccgg
     1261 gaaacgattt cgagctggcc gccggcttcg cgttcagcga gggcatcgtc acggcccgcg
     1321 aagagctggc gggcctgtcg tattgcctcg accccggcgt cgatcccgat cagcgttaca
     1381 acatcgtcaa cgtcgagctc gcgcggcgcg acttaccgga tttagaacgt ttcgaacgcc
     1441 acttcaccgt cggcagcgcc tgcggcgtct gcgggcgaac gcagctcgat tcgcttcgcg
     1501 atcgcggcgt gaagccgatc gaagagacga tgaccgtcgc gaccggcctc ctctactcgt
     1561 taccgggacg catgcgcgag gcgcagcgca tcttcgcctc gaccggaggc ttgcacgccg
     1621 ccgcgctctt cgacgagtcg ggcgaagccg tcgtcgtgcg agaagatatc ggccggcaca
     1681 acgcggtcga caagctcgtc ggctgggggc tgctcaacgg acgtctgcct tttacgcgct
     1741 cggtgctgat ggtgagcggc cgcgccggct acgagatcct ccagaagagc gcggtcgcgc
     1801 gcatcccggt cgtctgctcc gtttcggcac cgagcagcct cgcggtcgac ctggcgcgcg
     1861 agttcgagat cacgctcgcg ggtttcgtgc gcgacgaccg cgccaacgtc tacagccgcg
     1921 cggatcgaat tgcggccgcc cgatgacggc gccgtttgaa gtgctctagg cagacccgag
     1981 aaagtccccc aggagttcgt tgaaacggcc gggattgtcg gcgttggcca cgtggccggc
     2041 gccttcgacg atcgcgagcc gtgcgcccgg aatgccgcgc gcgatttctt ccgatagcgg
     2101 gtacggtgcg atcgcgtcgc gttcgccgca cagcacgagc gtggggacgg cgatggccgg
     2161 cagcagctcg cggtagtcgc cggtccacgt cgcttcggtc gaagcgagat agcacggtac
     2221 ggacttgcag gccatctgct cgatcgtctc ggcgaggcgt gccggcggca gtcccagctt
     2281 ggcggcccgc gcctcggcga aggcgcgcat gccgcccgcc tcgcggacga ttgccccgac
     2341 gccgtcggca tacgcgcgag cgttcggata gtacgcgaag ctgccgacga tcgccatcgc
     2401 gtcgaagcgt tcgggagcga gcttccatag ctcgaacgcg acgacgccgc cgaggctgca
     2461 gccgaccagc gtaaaccggc cggccccggc ggcgtcggcg accgccagga cgtcggcggc
     2521 gaaacccgct cgcgtgatca gcgacggatc gggatcggg
//
LOCUS       DASUCJ010000758         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1016788, whole genome shotgun sequence.
ACCESSION   DASUCJ010000758 DASUCJ010000000
VERSION     DASUCJ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016788"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="VFU90_09080"
     CDS             complement(<1..>1510)
                     /locus_tag="VFU90_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU5479972.1"
                     /translation="YDLVCVGGGISGLAAAVFFQKNRGGTCLVLENHPIFGGEAKRNE
                     FDVDGQRVVAHQGSAIFLVPQKGGYTDRVYEMIGMDRRAFEYQRWGGREPEMPLSNSP
                     YDEPKNYGFYFGSQFGQRPGVWVLDPWTRQLEGAPLSDAVKAELMRYRTVRDDGPRPQ
                     SEGDAISRQLDTITLEEHLMARHRISRETVRKFLSPIEGGGYGLGPDALSAYCAYAIE
                     TEFPEDGDDTLGDQMFPDGNTGLARLMVKTLIPETFAGPRTVDAVWRNPVRFSALDRA
                     GRPTRIRLNATVVRVEHEGDPAHASHVRVTYVKGGRLHRVRARGVVMAGGSWTTKHIV
                     HDLPTTHRDAYAQFYRSPCLMANIAVRNWRFLYKMGMSGCRWFGGLGDYLTVRKLALV
                     GGVRPTFGPDSPTVLTIKVLFARPGLPVGEQGSRGRQELLGTPFAQYERAFREQLGDM
                     FAPGGFDPRRDIAGIVLNRWGHAYVNPQPGFFFGVDGKPAPRDILRDRPHGRI"
BASE COUNT          254 a          519 c          482 g          255 t
ORIGIN      
        1 tatacggccg tgtggtcggt cgcgcaggat gtcgcgcggc gcgggtttgc cgtcgacccc
       61 gaagaagaat cccggctgcg ggttcacgta cgcgtgaccc caccgattga gaacgatccc
      121 cgcgatgtca cggcgcggat cgaagccgcc gggcgcgaac atgtcgccga gctgctcgcg
      181 gaacgcgcgc tcgtactgcg cgaacggtgt tccgaggagc tcctgccggc ctcggctccc
      241 ctgctcgccg acgggaagac cgggcctggc gaacaacacc ttgatcgtca acaccgttgg
      301 cgaatccggc ccaaacgtcg gcctcacgcc gccgaccagc gccagtttgc gcacggtgag
      361 gtaatcgcca aggccgccga accagcggca ccccgacatg cccatcttgt agaggaagcg
      421 ccagttgcgg acggcgatgt tcgccatgag acacggcgac cggtagaact gcgcataggc
      481 gtcgcggtgc gtcgttggca ggtcgtgtac gatgtgcttc gtcgtccagc tgccgccggc
      541 catgacgaca ccgcgcgcgc gaacgcgatg gagccggccg cccttcacgt acgtgacgcg
      601 cacgtgtgac gcgtgcgccg gatcgccctc gtgctcgacg cgcacgaccg tcgcattgag
      661 ccggatgcgc gtcggtcgcc cggcgcgatc cagtgccgaa aagcgaacgg gattgcgcca
      721 caccgcgtcc acggtgcgcg ggccggcgaa ggtctctgga atgagggtct tcaccatcag
      781 ccgcgcaaga ccggtgttgc cgtccgggaa catctggtcg cctaacgtgt cgtctccgtc
      841 ttcgggaaac tccgtctcga tggcgtaggc gcagtacgcc gatagcgcgt ccggtccgag
      901 cccgtacccg ccaccttcga tcggtgataa aaatttgcga accgtctcgc ggctgatgcg
      961 atgccgcgcc atcaggtgct cctcgagcgt gatcgtatcg agctgacgag agatggcgtc
     1021 gccttcgctc tgcggccgcg ggccgtcgtc gcgtaccgtg cggtagcgca tcaactccgc
     1081 tttcacggca tcgctcagcg gcgctccctc gagctggcgc gtccaggggt ccagcaccca
     1141 cacgcccgga cgttgcccga actgtgagcc gaagtaaaag ccgtagttct tcggctcgtc
     1201 gtacggactg ttgctgagcg gcatctccgg ctcgcgtcca ccccagcgct gatactcgaa
     1261 cgcgcgccga tccatgccga tcatctcgta gacgcgatcg gtgtagccac ccttctgcgg
     1321 gacgagaaag atcgccgaac cctggtgcgc gaccacgcgc tgcccgtcga catcgaactc
     1381 gttgcgcttc gcctcgcccc cgaagatggg atggttctcg agcaccaaac aggtgccgcc
     1441 cctgttcttc tgaaagaaca ctgctgccgc gagtccgctg atgccgccgc cgacgcagac
     1501 gaggtcgtac
//
LOCUS       DASUCJ010000759         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1383671, whole genome shotgun sequence.
ACCESSION   DASUCJ010000759 DASUCJ010000000
VERSION     DASUCJ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383671"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..>1117
                     /locus_tag="VFU90_09085"
     CDS             8..>1117
                     /locus_tag="VFU90_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479973.1"
                     /translation="MERRYRETESQMVREELAKYLSSQPCPECSGTRLRREARFVFVG
                     GESLPAISSRSVGDTLTFFDTLTLEGWRGEIAAKIVKEISARLSFLVNVGLDYLSLDR
                     SAETLSGGEAQRIRLASQIGSGLVGVMYILDEPSIGLHQRDNERLLQTLVRLRDLGNT
                     VIVVEHDEDAIRHADHVLDMGPGAGVHGGRVVAQGTPEEIAHNAASLTGQYISGRREI
                     ALPKARTRPDPKRQLRIRNARGNNLKGIDVEIPLGLMTCVTGVSGSGKSTLINDTLYR
                     YAARELNDASETPAACDGIEGLNQIDKIIDIDQSPIGRTPRSNPATYTGVFTPIRELF
                     AGTQEARSRGYQPGRFSFNVKGGRCEACQGDGVIKV"
BASE COUNT          217 a          351 c          357 g          192 t
ORIGIN      
        1 gccgaacatg gagcgccgct accgcgagac cgaatcgcag atggtgcgcg aggaactggc
       61 gaaatatctt tcgagccagc cctgcccgga gtgcagcggc acgcgtctgc gccgcgaagc
      121 gcgtttcgtg ttcgtcggcg gcgaaagcct gccggcgatt tcgtcacgct cggtgggcga
      181 tacgctgacg ttttttgaca cgctgacgct cgagggctgg cgtggcgaga tcgcggccaa
      241 gatcgtgaaa gaaatctccg cgcgactgtc gttcctggtc aacgtcgggc tggattatct
      301 gagcctcgac cgcagcgccg aaacgctttc aggcggcgaa gcgcagcgca ttcgcctcgc
      361 cagtcagatc ggctcggggc tggtcggcgt gatgtacatc ctcgacgaac cctccatcgg
      421 cctgcatcaa cgcgacaatg agcgcctgct gcagacgctg gtacggttgc gcgatctcgg
      481 caacacggtg atcgtggtcg agcacgacga ggatgcgatt cgtcacgcgg atcatgtact
      541 cgacatgggc ccgggcgccg gcgtgcacgg cggtcgcgtc gtcgcgcaag ggacacccga
      601 agagatcgcg cacaacgcgg cgtcgctgac gggacagtac atttccgggc ggcgtgagat
      661 cgccctgccg aaagcacgca cgcgcccgga tccgaaacgg caactccgga tccgcaacgc
      721 gcgcggcaac aatctcaagg ggattgacgt ggaaatcccg ctcggcctga tgacctgcgt
      781 gaccggcgtc tccggttccg gcaaatccac gctgatcaac gacacgctgt atcgttatgc
      841 cgcacgcgag ctcaacgatg cgtcggaaac accggccgcc tgcgacggca tcgaaggcct
      901 gaaccagatt gacaagatta ttgatattga tcagtcgccg atcggtcgca caccgcgctc
      961 caatcccgcg acttacaccg gagtcttcac gcccattcgc gaactgtttg ccggtaccca
     1021 ggaagcgcgt tcccgcggct accagccggg tcgtttcagt ttcaacgtga agggcggacg
     1081 ctgcgaagcg tgtcagggcg acggcgtgat caaggtc
//
LOCUS       DASUCJ010000760         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_69446, whole genome shotgun sequence.
ACCESSION   DASUCJ010000760 DASUCJ010000000
VERSION     DASUCJ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69446"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFU90_09090"
     CDS             <1..299
                     /locus_tag="VFU90_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5479974.1"
                     /translation="AIVHNAIAHAPARGHVRLKVARAGDAVRIEVADDGPGFTSAGLQ
                     HATERFWRGDSARPRGGTGLGLAIARTMVEANGGTLTLANSAGGGARVTVTLAG"
     gene            complement(309..854)
                     /locus_tag="VFU90_09095"
     CDS             complement(309..854)
                     /locus_tag="VFU90_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479975.1"
                     /translation="MIRLTFLGVALLLAAVPVYAHAQDMGAMSDSDYIAKVSTGAPAA
                     VVKGATIVKVNKDGTMRTLQSGTNGFTCLMVDTGTPGCADQNAMAWMKALMTHAAPPA
                     AVGFMYMLGGDSGASNTDPYATAPTRTNHWVKTGPHVMILGPAAASMGYPATADPDPT
                     KPYVMWASTPYAHVMIPVGGP"
     gene            complement(907..>1572)
                     /locus_tag="VFU90_09100"
     CDS             complement(907..>1572)
                     /locus_tag="VFU90_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5479976.1"
                     /translation="VVFCMDRAGFVGDDGPTHMGLYDIAYLRTLPNFTLMAPRCEAEL
                     LPMLEHALSLSGPSGIRYPRGSSSGRHDEPSAPIVHGKAQILRRGRGVAILAYGITAD
                     VALDAYDILRDGGEPQEADLPTVVNARFAKPLDEALLLELERDHGRIVTLEEHSSAGG
                     FGAAVVEFVSDRGLKLTVERVGVGNVLVQHDSQDKQRAMFGLNAASLAARVAGMASIP
                     AAR"
BASE COUNT          280 a          532 c          489 g          271 t
ORIGIN      
        1 tggcgatcgt ccacaacgcc atcgcccacg cacccgcgcg cggacacgtc cgcttgaagg
       61 tggcacgagc gggtgacgcc gtccgcatcg aagtagccga cgatggtccg ggctttacgt
      121 cggccggcct gcagcacgcc accgagcgtt tctggcgagg tgactcggcc cgcccacgcg
      181 gcggaaccgg cttagggctc gcaattgcgc gcacgatggt cgaagccaac ggcggaacgc
      241 tgaccctcgc caattcggcc ggtggcggcg ctcgcgttac cgtaacgctg gccggttagg
      301 gcgccccctt agggtccgcc gaccggaatc atgacgtgtg cgtacggcgt actagcccac
      361 atgacgtacg gtttcgtcgg atcgggatct gccgtcgccg ggtagcccat cgaggccgcc
      421 gccggtccga gaatcatcac gtgcggtccg gtcttgaccc agtgattggt ccgcgtcggc
      481 gccgtcgcat acggatcggt attgctggcg ccggaatcgc cgccgagcat gtacatgaat
      541 cccaccgcgg ccggcggtgc ggcatgcgtc atgagcgcct tcatccacgc catcgcattt
      601 tgatcggcac agccgggcgt ccccgtatcg accatcaggc aggtaaatcc gttggtcccc
      661 gattgcaggg tgcgcatcgt gccgtctttg ttgaccttca cgatcgtcgc acccttgacg
      721 accgccgccg gcgcgccggt cgaaaccttg gcgatgtaat cggaatcgga catcgctccc
      781 atgtcctgcg catgcgcgta caccggaacg gctgccagca agagcgctac gccgagaaac
      841 gtcaaacgaa tcatccgcta actccttcga aaagccgctt cacaagaatg gatttatgaa
      901 gccgaactaa cgggcggccg gaatgctcgc catccccgcg acgcgcgccg cgagggatgc
      961 ggcattcaac ccgaacatcg cccgctgctt gtcttgtgag tcgtgctgca cgagtacgtt
     1021 gccgacgccc actcgctcga ccgtgagttt caaaccccgg tcggacacga actcgacgac
     1081 cgccgcgccg aaccctccgg ccgacgagtg ctcctcgagc gtcacgatgc ggccgtggtc
     1141 gcgttccagc tcgagcagca acgcttcgtc gagcggtttg gcgaaacgag cgttgaccac
     1201 cgtgggaaga tcggcttctt gcggctcgcc gccgtcgcga aggatatcgt aagcatcgag
     1261 cgccacatcc gccgtgatac cgtaggcgag gatcgcgacg ccccgccccc ggcgcaggat
     1321 ctgcgccttg ccgtgtacga tcggcgcgct cggttcgtcg tgtctcccgc tgctggaacc
     1381 gcgaggatac cggattcccg atgggccgct gagcgacagc gcgtgttcga gcatcggcaa
     1441 cagctcggct tcgcagcgcg gagccatcag cgtaaaattg ggcagcgtcc gcaggtaggc
     1501 aatgtcgtaa agtcccatgt gggtcggtcc gtcgtcgccg acaaacccag cccggtccat
     1561 gcaaaaaacc ac
//
LOCUS       DASUCJ010000761         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_54066, whole genome shotgun sequence.
ACCESSION   DASUCJ010000761 DASUCJ010000000
VERSION     DASUCJ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54066"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VFU90_09105"
     CDS             complement(<1..1480)
                     /locus_tag="VFU90_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5479977.1"
                     /translation="MSVSSPFIHRPIATSLLGVAVMLGGLLGYLWLPVSALPQVDFPT
                     IQVTTQLPGANPQTMSTLVTASLERELGQIPALSTMTSTSAYGLSQITLQFNLGRDID
                     AAAQDVQSAINAAGSTLPRNLPYPPTYSKVNPADAAIVTLALTSDSVSMRDLSDLADT
                     LIAQRLSTVTGVGHVSVEGGIRPAVRIDADVTRLAAYGLSMTDLSNAIVGANVAGPKG
                     SLDGALQSYTIAANDQITSADAYRDIVVAYRNDGPVRLRDVASVEDGLENVNVGGWYN
                     GTPAVIVDVQRQPGANVIETVDRLKAELPRLKRALPPSVTLTLVHDRTGTIRASMHDV
                     QFTLCLSVALVVMVVLIFLRSTRATIIAGVALPVSLIATFGVMWFCGFSLDNLSLMAL
                     TIGTGFVVDDAIVMIENIVRHLEKGARPLHAALNGAREIGFTVISLTMSLIAVFIPLL
                     FMPGLPGRLFREFAVTLSVAVLISLVISLTTTPMMCAWVLRRG"
BASE COUNT          287 a          469 c          542 g          234 t
ORIGIN      
        1 cgccgcggcg cagcacccat gcgcacatca tcggcgtcgt cgtgagggag atcacgagcg
       61 agatcagcac cgcgaccgac agcgtcaccg cgaactcgcg gaagagccgt ccgggaagcc
      121 ccggcatgaa cagaagcgga atgaacaccg cgatcagcga catcgtgagc gagatgaccg
      181 tgaagccgat ctcgcgggcg ccgttgagcg ctgcatgaag cggccgcgcg cccttttcga
      241 ggtggcgcac aatgttctcg atcatgacga tggcatcatc gacgacgaaa ccggtgccga
      301 tcgtcagcgc catcagcgaa agattgtcga ggctgaaccc gcagaaccac atgacgccga
      361 aggtggcgat gagcgacacg ggcagcgcca cgccggcgat gatggtggcg cgcgtcgacc
      421 gcaggaagat cagcaccacc atcacgacca gcgcaacgct taagcacagc gtgaactgca
      481 cgtcgtgcat ggacgcgcga atggtgccgg tgcggtcgtg gacgagcgtg agagtcacgc
      541 tcggcggcag cgctcgcttg agccgcggca gctccgcctt cagccggtcg acggtctcga
      601 tgacgttggc gccgggctgt cgctgcacat cgacgatgac ggccggcgtc ccgttatacc
      661 agccgccgac gttgacgttc tccaggccgt cctcaacgct ggcgacgtcc ctcagccgga
      721 ccggcccgtc gttgcgatag gcgacgacga tatcgcggta ggcatcggcg gaggtgatct
      781 ggtcgttcgc cgcgatggtg taggactgca gcgcaccgtc gagcgatccc ttggggccgg
      841 ccacgttggc gccgacgatg gcgttgctca aatccgtcat gctcaggccg taggcggcga
      901 gccgggtaac atcggcgtcg atgcgaacgg cgggccggat gccgccctcg acggagacgt
      961 ggccgacgcc ggtcactgtg gagagccgct gggcgatgag cgtgtcggcg aggtcgctta
     1021 ggtcgcgcat cgagaccgaa tccgacgtga gcgcgagcgt gacgatcgcc gcgtcggcgg
     1081 gattgacctt cgagtacgtc ggcggatagg gcagattgcg cggcagcgtg gagcccgccg
     1141 cgttgatggc cgactgcacg tcctgcgcgg cggcatcgat atcacggccg aggttaaact
     1201 gcagcgtgat ttggctgagg ccgtaggcgc tcgtcgacgt catggtcgag agcgcaggga
     1261 tctggccgag ctcgcgctcg agcgaggccg tcaccagcgt cgacatcgtc tggggattgg
     1321 cgccgggaag ctgcgtcgtc acctggatgg tgggaaagtc gacctgcggc agcgccgata
     1381 ccggaagcca cagataaccc aagagcccgc cgagcatcac cgcgacgccg agcagcgacg
     1441 tcgcgatcgg tcggtggatg aagggcgagg agacgctcat atcgaatgac tacgctgttt
     1501 gggcattgag gcgccgcgag ctctgactat tg
//
LOCUS       DASUCJ010000762         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1169651, whole genome shotgun sequence.
ACCESSION   DASUCJ010000762 DASUCJ010000000
VERSION     DASUCJ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169651"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFU90_09110"
     CDS             <1..396
                     /locus_tag="VFU90_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479978.1"
                     /translation="DRRTVGARSGFAMYAASKLMNILHARELARRLDSTRVTSYAVHP
                     GAVASNIWRSLPWIIRRALMLFLISNEEGARTQVWCAAAPELASSSGRYYYDGKEARG
                     SALSRDAALAAELYTRTERAIADTLGLTA"
     gene            complement(409..1104)
                     /gene="msrA"
                     /locus_tag="VFU90_09115"
     CDS             complement(409..1104)
                     /gene="msrA"
                     /locus_tag="VFU90_09115"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5479979.1"
                     /translation="MYAAFVRTTSLLGMALGAAVFVAWPTTADAGRDPVPNRAVQTPR
                     PANLETAVLAGGCFWGMQLVFGHVKGVTAVAAGYAGGDKSTATYPQVETGQTGHAESV
                     RIVFDTTQVTYRQILQVYFMVAHDPTELNRQGPDAGTQYRSAIFYATPEQQQIAQAYI
                     AQLEKDKTFDSPIVTQVVPLKGFYPAEKYHQNYAVHHPDQPYIVINDLPKVASLREQL
                     PALYRDQMVQATS"
     gene            1282..2019
                     /locus_tag="VFU90_09120"
     CDS             1282..2019
                     /locus_tag="VFU90_09120"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU5479980.1"
                     /translation="MMLRHVFGATVALCLFSTAAVAAQQTDTTKKTIKFTGNFGLVNA
                     AGNSSVTTLNTDERIAWTAGVWGITESFSVIYGKNDSATTASTWSGLLRGDRALGSRL
                     SLYVLGTYDRNTFAGFDRRFEEGLGVSVQALSTPRDSINAELGVSYTEQHNTDSTSND
                     FPAARVAGTYTHTFSKTAYFKQGVDVLPDLKVGRNLRFNTESALVASLSANIALKVSY
                     VVHFDNLPQPGFKKSDRLLATGVQVTF"
     gene            complement(2081..2848)
                     /locus_tag="VFU90_09125"
     CDS             complement(2081..2848)
                     /locus_tag="VFU90_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479981.1"
                     /translation="MRTGQVRWVAATAAVLALAGTAAGSARAQAPSSGDQVIRAMHDR
                     YAGKWYKTLTFQQKTTRRTPADTMEIQTWYETAEVPGRLRIDIAPRGGMSYIYANDSI
                     YVTNKDSVLRRLPGRNPLLILGFDVYGQPAEKTIDVLKDEGFNLGPVHEDTWEGRPVY
                     VVGAAKGDTTSKQFWVDKDRLLFVRLLEPSPRDSSKEGDTRFDDYRPLGHGWISALVE
                     FYSGGKLMQREEYSDIKADPRIPANIFTPGSTPAPMK"
     gene            complement(2859..3650)
                     /locus_tag="VFU90_09130"
     CDS             complement(2859..3650)
                     /locus_tag="VFU90_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5479982.1"
                     /translation="MNLGLDGKVALVAAASRGLGRAIATELAAEGATISMCARDAAAL
                     ERARHDIESKTGRPVLAVTADVSRSDDVVRFVDETLARFGRVDVLVTNAGGPPAGLFE
                     AHDAAAWDNAFRLTLASAVELARRVLPGMKERRWGRIINVTSIAVKQPVDYLILSNSL
                     RAAVTGFARTLATEVAPFGITVNNVLPGYTRTERMEALAAARNKGEGISREAAFADWE
                     KQIPARRLGEPDELAALTAFLASERAAYITAQSIAVDGGWIRALY"
     gene            complement(3654..4034)
                     /locus_tag="VFU90_09135"
     CDS             complement(3654..4034)
                     /locus_tag="VFU90_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5479983.1"
                     /translation="MNDASKAVWYCWDKMPKERVSDTLERRLVTGERMMLAHVYLKKG
                     AVVPKHSHDNEQLTYILEGALKFWLGDNGERELVVGAGEVLYIPSNLPHKAEALHDTL
                     DVDVFSPPRADWLDKTDAYLREKR"
BASE COUNT          676 a         1403 c         1322 g          740 t
ORIGIN      
        1 gaccgccgca ccgtcggcgc gagaagcggc ttcgccatgt acgcggcgtc caagctcatg
       61 aacatcctgc acgcacgcga gctcgcgcgg cgactcgact cgacccgcgt cacgagctat
      121 gcagtgcacc cgggcgccgt cgcctcgaac atctggcggt cgctgccctg gatcatccgg
      181 cgcgcgctga tgctgttcct gatctcgaac gaagaggggg cgcgcacgca ggtctggtgc
      241 gcggccgcgc ccgagctcgc gtcgagctcg ggccggtatt actacgacgg caaggaagcg
      301 cgcgggtcgg cgctctcgcg cgacgcagcg ctcgcggccg agctctacac ccggaccgag
      361 cgcgccatcg cggacacact cggcctaact gcctaacgga ctaacggatc agctggtcgc
      421 ctgcaccatc tggtcgcggt acagcgccgg cagctgttcg cgcaggctcg ccaccttcgg
      481 caggtcgttg atcacgatgt agggctgatc gggatggtgc accgcgtagt tctggtgata
      541 cttctcggcc ggatagaagc ccttgagcgg caccacctgc gtgacgatcg gactgtcgaa
      601 cgtcttgtcc ttctccagct gcgcgatgta cgcctgggcg atctgctgct gctcgggcgt
      661 cgcgtagaaa atcgccgacc gatattgcgt gccggcatcc ggcccctgcc ggttgagctc
      721 ggtggggtcg tgcgcgacca tgaagtacac ctgcaggatc tgccgatacg tcacctgcgt
      781 ggtgtcgaac acgatgcgca ccgactcggc gtgtccggtc tggcccgtct ccacctgcgg
      841 gtacgtggcc gtactcttgt cgccgcccgc gtaccccgcg gcgacggcgg tcacgccctt
      901 cacgtgcccg aacacgagct gcatgcccca gaagcagccg cccgcgagca cggccgtttc
      961 cagattggcc ggacgcgggg tctgcaccgc tcggttaggc accgggtcgc ggccggcgtc
     1021 cgcggtcgtc ggccaggcga cgaacacggc cgcgcccagc gccatgccca acagagacgt
     1081 cgtgcgaacg aaggcagcgt acatagcggt ggacctcagg tatcgggcgt cggatgagca
     1141 cgccgcaccc gcaacgcgcg ccgtcggact cccttgtcga acatgataca cctaactaac
     1201 gaacgagatt cgcgcacggg aagtgcccgc gtcgttggcg ctcacagccc gccaggttca
     1261 ccctccggtc agggatcgtc catgatgctt cgacacgtgt tcggcgcgac ggtcgcgctc
     1321 tgcctttttt ctacagccgc ggttgccgcg cagcaaaccg acaccaccaa gaaaaccatc
     1381 aaattcaccg ggaacttcgg cctggtgaac gcggcgggca actccagcgt caccacgctg
     1441 aataccgacg agcgcatcgc ttggaccgcc ggtgtctggg gcatcacaga aagcttctcc
     1501 gtgatctacg ggaagaacga ttcggcgacg accgccagca cgtggagcgg actgctgcgc
     1561 ggcgaccgcg cgcttggctc ccggctgtcg ctgtatgtgt tgggcacgta cgaccgaaac
     1621 acctttgccg gattcgaccg ccgcttcgag gaagggctcg gcgtcagcgt tcaggcgctg
     1681 agcacgccgc gcgattcgat caacgcggag ctcggcgtgt cgtacacgga acagcacaat
     1741 acggacagca cctccaacga tttcccggcc gcgcgcgtcg cgggcaccta cacgcacacg
     1801 ttcagcaaga cggcgtactt caagcagggc gtcgacgtcc tgccggacct caaggtcgga
     1861 cgcaatctgc gcttcaacac cgagagtgcg ttagtcgcat ccctctccgc gaacatcgcg
     1921 ctcaaggttt cgtatgtcgt gcatttcgac aacttgccgc agcccgggtt caagaagagc
     1981 gaccggttgc tggccacggg cgtgcaggtg accttctgag ccgatgcgac gacggcgtcc
     2041 ggcatcgcgc cgggcgccgt ccgccgcacg cctcccgcat ttacttcatc ggcgccggcg
     2101 tcgagcccgg cgtaaagata ttcgccggga tcctcggatc cgctttgatg tccgagtatt
     2161 cttcgcgctg catcagcttc ccgccggaat aaaactcgac gagcgccgag atccatccgt
     2221 ggcctaacgg acggtagtca tcgaagcgcg tgtctccttc cttggaggaa tcgcgcggcg
     2281 acggctcgag caggcgcacg aagagcagcc gatccttgtc cacccagaac tgcttcgacg
     2341 tcgtatcgcc cttggccgcg cccacgacgt acacgggacg tccctcccat gtgtcctcgt
     2401 gcacggggcc gaggttgaac ccttcgtcct tgaggacgtc gatggttttc tcggccggct
     2461 gcccgtacac atcgaagccg agtatgagca gcggattgcg gccgggcagg cgacgcagca
     2521 cgctgtcttt gttcgtcacg tagatgctgt cgttggcgta gatgtacgac atgccgccgc
     2581 gcggcgcgat gtcgatgcgc agccgtcccg ggacttctgc cgtctcgtac cacgtctgga
     2641 tttccatcgt gtcggccggc gtgcggcgcg tcgtcttctg ctggaaggtg agcgtcttgt
     2701 accacttgcc ggcgtaccgg tcgtgcatgg cgcgaatcac ctgatcgccc gacgagggcg
     2761 cttgcgcccg ggcggatccc gccgccgtgc cggccaacgc cagcacggcc gcggtggcgg
     2821 cgacccatcg gacctgtccg gttctcatgc gtcctccgtt agtaaagtgc gcggatccac
     2881 ccgccgtcga cggcgatcga ctgcgccgtg atgtacgcgg cgcgctccga tgccaggaag
     2941 gccgtgagcg cggccaactc gtccggttcg cctaaccggc gcgccggaat ctgcttttcc
     3001 cagtcggcga acgccgcctc gcggctgatg ccctcgccct tgttgcgcgc cgccgccagc
     3061 gcctccatgc gttcggtgcg cgtataaccc ggcagcacgt tgttcaccgt gatgccgaac
     3121 ggtgccacct cggtggccag cgtacgcgcg aatcccgtca cggccgcgcg caagctgttg
     3181 gacaggatca gataatccac cggctgtttc accgcaatcg atgtcacgtt gatgatgcgt
     3241 ccccagcggc gctctttcat cccgggaagc acacggcgcg ccagctcgac ggccgacgcg
     3301 agcgtcaaac ggaacgcgtt gtcccatgct gcggcgtcgt gcgcctcgaa gagacccgcg
     3361 ggcggaccgc ccgcattggt gaccaataca tccacgcgtc cgaatcgcgc gagcgtctca
     3421 tcaacgaatc gcacgacgtc gtcggatcgc gacacatctg ccgtgaccgc gaggaccggt
     3481 cgccccgtct tcgactcgat gtcgtgccga gcgcgttcga gtgcggccgc gtcgcgagcg
     3541 cacatcgata tcgtggcgcc ctcggcggcc agctctgtgg cgatcgcgcg tcccaatcct
     3601 ctgctggccg ccgcgaccag cgccaccttt ccgtcgagac cgaggttcat gggtcagcgc
     3661 ttctcgcgaa gatatgcgtc ggttttgtcg agccagtctg cgcggggcgg tgagaacacg
     3721 tcgacgtcga gcgtgtcgtg caaggcttct gctttgtgcg gaagattgga tgggatgtac
     3781 agaacttcac cggcgccgac gacgagctcg cgttcgccgt tgtcgcccag ccagaacttg
     3841 agcgcgccct cgaggatgta cgtgagctgc tcgttgtcgt gcgagtgctt gggcacgacc
     3901 gcgcctttct tgagatagac gtgcgcgagc atcatgcgct cgccggtcac caggcgccgc
     3961 tcgagcgtat cggacacacg ttctttgggc attttgtccc agcagtacca cacggccttg
     4021 ctcgcgtcgt tcacggcgat tctccgtcag agggtgttgg tgcggtgcgt ccgggagaga
     4081 tagcaaacgg tgcggcacgg cgcgagcgcg ccgcgcctcc gacggcaggc agattcggcg
     4141 g
//
LOCUS       DASUCJ010000763         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_176089, whole genome shotgun sequence.
ACCESSION   DASUCJ010000763 DASUCJ010000000
VERSION     DASUCJ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176089"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFU90_09140"
     CDS             complement(<1..1116)
                     /locus_tag="VFU90_09140"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEU5479984.1"
                     /translation="MVTMRRRRAASAMVAVVALLGALLGARRPSARPAPICDPPSVSQ
                     PEPALDAPTRPDWLLAEHRYIALARWLAAASARPGTPASTLDYVRGVLANKENENAES
                     IRDLEPLVPRLDPVADSARFRVVLKTLADDYLKTYRYGDAANALDRLYRASGATMAPA
                     DRTDLRSELLLRRALRNAPRQRTVVASPFAIPLTPDAIGLRDVTVHVGRDSSKWIFDT
                     GANLSTITETVARRLRLHVITSGSTTSGITGVAVPSRVAVIPEMRIGAARVEHAVVLV
                     MPDRMLDIPQIKFQITAILGYPVLEALGRLAIGTDSLRVDPAAGEAPSDSSNLFLDDL
                     SPVVSATVGDSTRLFHFDSGADATMLSVRFCRAYSTLL"
     gene            complement(1332..2084)
                     /locus_tag="VFU90_09145"
     CDS             complement(1332..2084)
                     /locus_tag="VFU90_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479985.1"
                     /translation="MDEKLRKEVRILKAYAILSSLLFAVLLFTAFRSPKTVDFDQINA
                     HRINIRETNGTIRLAISNTDEFPGGTIGGVELKYRNGHRGAGLLFFNDDGDENGGLTY
                     ETHVVNGVPEASSTIRFDHFRQQEAVGLDYEQHGTTKQSGLLVWDQPNEPLTGDMARA
                     LNDIQAMKDTAAQRAAFQSFQRKYDKAFGHFTQRVFVGRTKNDDAAVLLMDKRGKARV
                     KLAVDSANNAFLEFLDDSGKVVYRLPNDSTGT"
     gene            complement(2332..3543)
                     /locus_tag="VFU90_09150"
     CDS             complement(2332..3543)
                     /locus_tag="VFU90_09150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5479986.1"
                     /translation="MSIGSVASRGVLTITPWYGGVEGGVGVATETISHTLLRTGSRCA
                     VIEMAADAVVPRLRRGRAGELIVGMCLRPDTSLTGTLRARIGYRVRQATAMGGLAMLT
                     ARFDANVAHFHYVNDSYIPLFAICRRLGLATVLTLHGTDVNDADGNAWARVDRLLRAA
                     DAVTTVSAALFRTIVARSPEMADKTAVVPNSVPVDITAAAEAVPGHRSEPAIDVLFLG
                     NLRVVKGPDVLLEAMQLVSARIGNVSVTIAGAGPLREQLMQRSRQIGIDHLVTFRGRV
                     SREEAVGLLCRSRILVIPSRSEGAPLVALEAQMLGVPIVATNVGGIPESVTQDGSGML
                     CPPNDPTALASAVLQLLGDDALRRRLGAEGRQRAATLFSPERIASRYHEVYEQAVARR
                     RAPPLLIAAAR"
     gene            4039..4899
                     /gene="hisG"
                     /locus_tag="VFU90_09155"
     CDS             4039..4899
                     /gene="hisG"
                     /locus_tag="VFU90_09155"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU5479987.1"
                     /translation="MLRIALPNKGRLADDARELFRDAGVDVRARDERALTSTLGDLFE
                     AIFVRAQDIPEFVADGAADAGVTGADLIAESDRALHTRADLEFGRCRLVVAATDASDI
                     RAVEAIPQGARVATSFPRLATRFFAAARRTVEIVPVSGASEIAPHIGIADVIVDLVAT
                     GSTLRVNGLHEVATILESSARMVTASVPRRPDCGRALDDLVGALESVLRARGQRYLMA
                     NVPRARLTQVRRVLPGLNGPTVVDIMNSGEHVAVHAVVPASGVYRIVAELKALDAEGI
                     LVTRIERLMP"
     gene            4896..>5095
                     /locus_tag="VFU90_09160"
     CDS             4896..>5095
                     /locus_tag="VFU90_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479988.1"
                     /translation="MSVAVAVPMRYTGRVRDLTPAVYRWLCDRSTSSDPGVRESVASL
                     IADVRARGDTALRELAQRYDDAS"
BASE COUNT          815 a         1744 c         1715 g          821 t
ORIGIN      
        1 gaggagcgtc gaatacgcgc ggcagaaacg caccgacagc atggtcgcat cggcgccgct
       61 gtcgaaatga aagagacgcg tcgaatctcc caccgttgcc gacacgacgg gtgacagatc
      121 gtccaggaac agattcgatg aatcactcgg cgcctcgccc gccgccggat cgacgcgcag
      181 gctgtccgtg ccgatcgcga gtcggcccaa cgcctccagc acgggataac caaggatggc
      241 ggtgatctgg aacttgatct gcggaatgtc gagcatgcgg tcgggcatca cgagcacgac
      301 ggcgtgctcg acgcgcgcgg cgccgatgcg catctcggga atcacggcca cgcgagacgg
      361 gacggcgacc cctgtgattc cgctcgtcgt cgatccactc gtgatcacgt ggaggcggag
      421 ccgccgcgcc accgtttcgg tgatggtcga caggttcgcg ccggtatcga aaatccattt
      481 ggacgaatcc cggccgacat ggacggtgac gtcgcgcagt ccaattgcgt caggcgtgag
      541 cggaattgca aacggcgacg ccacgaccgt gcgctgcctg ggcgcgttgc gcagcgcgcg
      601 gcgcagcagc aactcgctgc gcaggtcggt gcggtccgcc ggcgccatcg tcgcgccgct
      661 cgcccgatag aggcgatcga gcgcgttggc ggcgtcgcca taccgatacg tcttcaagta
      721 gtcgtcggcg agcgtcttca acaccacgcg gaaacgcgcc gagtcggcaa cgggatcgag
      781 acgcggcacc agcggctcga gatcccggat cgactccgcg ttctcgttct ccttgttggc
      841 gaggaccccg cgtacgtagt cgagcgtcga cgccggcgtg cccgggcgcg cgctcgcagc
      901 cgccagccac ctcgcgagcg cgatgtaccg gtgctcggcg agcagccagt cgggccgggt
      961 gggcgcatcg agcgccggct cgggctgact aacggacggc ggatcgcaga tgggagcggg
     1021 gcgcgcgctc ggccgacgtg cgccgaggag cgcaccgagc agcgcgacga ccgcaaccat
     1081 cgccgacgca gcacgtcgcc gacgcatcgt gaccatgcaa cacctcggac cgagaacgat
     1141 tggtcgtgcc taacgcgcga gccgaggatc ggcccgatcg accaagatga caggagaaaa
     1201 attggcccgg gcaatcgcgc tgtcaacgcg cccggagctc ggcatcacga gatgtgcgag
     1261 ctgggtgccc gcgcgccgcg ccgcgcgcgc ggatccgccc gcgccctgtc cgccgactga
     1321 cgccggccgg actacgttcc ggtcgagtcg ttaggcaagc ggtacacgac cttcccgctg
     1381 tcgtccagga actcgaggaa cgcgttgttc gcgctgtcca ccgccagctt gacgcgcgcc
     1441 ttccccctct tgtccatgag cagcaccgcg gcgtcatcgt tcttcgtccg cccaacgaac
     1501 acgcgctgcg tgaagtggcc gaacgccttg tcgtattttc gttggaacga ctggaatgcc
     1561 gcgcgctggg ccgcggtgtc tttcatggcc tggatgtcgt tgagcgcgcg ggccatgtcg
     1621 ccggtgagcg gttcgttcgg ctgatcccac accagcaggc cggattgttt cgtcgtcccg
     1681 tgctgctcgt agtcgagacc cacggcttcc tgctgacgga agtggtcgaa gcgaatggtg
     1741 ctgctcgcct cgggcacgcc gttcacgacg tgcgtctcgt acgtgaggcc gccgttctcg
     1801 tcgccgtcgt cgttgaagaa gagcagtccc gccccgcggt gtccgttgcg gtacttgagc
     1861 tcgacgccgc cgatggtgcc gccgggaaat tcgtcggtgt tggaaatggc gaggcggatc
     1921 gttccgttcg tttcgcggat gttgatgcgg tgggcgttga tctgatcgaa gtccacggtt
     1981 ttcggcgagc ggaacgcggt gaagagcagc acggcgaaca agagcgacga gaggatggcg
     2041 tacgccttca ggatgcgcac ttccttgcgg agtttctcat ccatggtggg tctccgtcgg
     2101 tgccgtcgct ggtggcagag ggggtgcgcg gcggcggatg gcaggttgtc cggaccgctg
     2161 gggccggcgg gcgatatgtc cggacaaata tagggacggt gcgctataat aatagtagga
     2221 acagctgtgc ccgcttttgc ccgatccgtg cgacccttcg cgcctcgagc gcgtgcctgt
     2281 gtccgcgggc gcgtggacgg gccgcccgac ccgggcgttg tggcaaaaag cctaacgcgc
     2341 agccgcgatc agcaacggtg gcgcgcgccg ccgggcgacg gcctgctcgt acacctcgtg
     2401 gtatcgcgac gcgatccgct cgggcgagaa cagcgtcgcg gcccgctggc gcccctcggc
     2461 gccgagccgg cggcgaagcg cgtcgtcgcc gagcagctgc agaaccgccg acgcgagcgc
     2521 cgttggatcg ttaggcggac agagcatgcc gctgccatcc tgcgtcacgc tctccggaat
     2581 cccgccgacg ttggtggcga cgatcggcac gccgagcatc tgcgcctcga gcgcgacgag
     2641 cggagcgcct tcggaccggc tggggatcac gaggatccgc gagcggcaca acaggccgac
     2701 ggcttcctcc cggctcacac ggccgcggaa cgtgaccaga tgatcgatgc cgatttgccg
     2761 cgaccgctgc atcagttgct cccgcagcgg accggcgccc gcgatcgtga ccgagacgtt
     2821 cccgatccgc gcgctcacca gctgcatggc ctcgagcagg acatcgggac ccttcacgac
     2881 acgcagattg ccgaggaaca gcacgtcgat ggccggttcg ctccgatgcc ccggcacggc
     2941 ctcggcggcg gccgtgatgt ccaccggcac ggagttgggc acgaccgccg tcttgtcggc
     3001 catctcgggc gatcgcgcaa cgatggtgcg gaagagtgcc gccgatacgg tggtcacggc
     3061 gtcggcggcg cggagcagtc ggtcgacgcg ggcccaggca tttccgtcgg cgtcgttgac
     3121 gtcggtgccg tgcagcgtca gcaccgtggc gagtccgagc cgccggcaga tggcgaagag
     3181 cgggatgtac gaatcgttca cataatgaaa atgcgcgacg ttcgcatcga agcgtgccgt
     3241 gagcatggcg agtccgccca ttgccgtcgc ttgccgcacg cggtacccga tacgggcgcg
     3301 cagcgttccc gttaggctgg tgtcggggcg caggcacatg ccgacgatca gttcgcccgc
     3361 acgcccgcgt cgcaggcggg gtacgacggc gtcggccgcc atctcgatca cggcgcagcg
     3421 cgagcccgtg cgcaacagtg tatgggagat ggtttccgtg gcgacgccga cgccgccttc
     3481 cacaccgccg taccacggcg tgatggtcaa gacgcctcgc gatgcaaccg aaccaatgga
     3541 catcacggcc tgtgatcctc gcgggacgcg tagcgggtcg caacgttccg aagatcggcg
     3601 tctgcgagtc acgggtcaag ctcatgcgtc gtgcagcgtc cggtgggtac cgagctgagc
     3661 gacggcactt cgcggtccaa ccggttcacg ttaggcgatc cggcgggacc tcgaacggcc
     3721 ccaccaccga gcgatccgtg ccgttctccg tgctgttctc cgttcaatcc gctctcagga
     3781 agcccgccac ggcgatgctc acgccactcg ggcgcgacca acgagttttt gtctcccgca
     3841 ctttgacacc cgcacgtcca tcgcatatgt tattggcatg cacattgacc gcgtcaccca
     3901 ccatcacgcg cttcaccacc acggcccgac gggtcgcggc ggagcgcgca tgcggatgac
     3961 tcggtaagca tccaccattc acgctctgct cgaggcccgt ccagttctgg acgggccttt
     4021 tctgtttgcc caacgaccgt gctacgaatc gccctgccca acaaaggccg actggccgac
     4081 gacgcccgcg agctgtttcg cgacgccggc gtcgacgtcc gcgcgcgcga tgaacgcgcg
     4141 ctcacgtcca ccctcggcga cctgttcgag gccatcttcg tccgcgccca ggacattccc
     4201 gagttcgtcg ccgacggcgc cgcggacgcc ggcgtcaccg gcgccgacct catcgccgaa
     4261 tccgatcgcg ccctccacac ccgcgccgat ctcgagttcg gtcgctgccg cctcgtcgtg
     4321 gccgcgaccg acgccagcga cattcgcgcg gtcgaggcaa tcccgcaggg agcgcgtgtc
     4381 gcgacgagct ttccgcgcct cgccacacgc ttcttcgcgg ctgcgcgccg caccgtggaa
     4441 atcgtgccgg tgtcgggcgc gtccgagatc gcgccgcaca ttggcatcgc ggacgtgatc
     4501 gtggatctcg tcgccaccgg ctccacgctg cgcgtgaacg gactgcacga agtcgccacg
     4561 atcctcgaat cctcggcgcg catggtgacg gcgagcgtcc cgcgccgccc ggactgcgga
     4621 cgcgcgctcg acgatctcgt cggcgccctc gagtccgtgc tgcgggcgcg cggccagcgc
     4681 tacctcatgg ccaacgtgcc gcgcgcccgc ctaacgcaag tcaggcgcgt gcttccggga
     4741 ctcaacggcc caacggtggt cgacatcatg aacagcggcg aacacgtcgc cgtgcacgcc
     4801 gtggtgccgg cgtcgggcgt ctaccgcatc gtcgccgagc tcaaggccct ggacgccgag
     4861 ggcattctcg tcacacgcat cgagaggctc atgccatgag tgtcgccgtg gcggtcccca
     4921 tgcggtacac cggacgcgtt cgcgatctca cgccggccgt gtaccgatgg ttgtgcgatc
     4981 gctccacgtc gagcgatccc ggcgtccgcg agagcgtggc atcgctgatc gccgacgtgc
     5041 gcgcccgtgg agacacggcg ctgcgcgagc ttgcccaacg ctacgatgac gcgtc
//
LOCUS       DASUCJ010000764         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1353260, whole genome shotgun sequence.
ACCESSION   DASUCJ010000764 DASUCJ010000000
VERSION     DASUCJ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353260"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFU90_09165"
     CDS             <1..345
                     /locus_tag="VFU90_09165"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479989.1"
                     /translation="VWGPGQRTPIHDHCTWGVIGMLRGEEIGRPYRLERGRVVPDGPE
                     ERLVPGQTMRVSPSIGDIHTVRNAFDDRTSISIHVYGADIGKQKRHVYDATSATVKMF
                     VSGYSNHPDYGK"
     gene            complement(365..>1439)
                     /locus_tag="VFU90_09170"
     CDS             complement(365..>1439)
                     /locus_tag="VFU90_09170"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5479990.1"
                     /translation="VEFDAAPTMTVVIPAYNEGPTVERAIDSIAGARYPAERLQIVVV
                     DDGSNDDTWEYIMRAAVRHSGRVTTLRLARNRGKREALAAGFRIARGAILVTVDSDSM
                     IERDALLAMAGQFRDPRIGAVAGKVAAWNRDRGVISRMLHVRFILSFDVQRSVQSTCR
                     TVYCCPGALAAYRASVVEQVLEPWLAQRFLGARCTFGEDRALTNDIMACGFDTVYQSN
                     AVVQTLVPTTYAGLCRMYLRWDRSHVREELRFLRIVWKRPLASMLFAALEKTMFGVGV
                     PIGWIGLAVLAAAIAADPAIALKVLLAIGIGAAFSMLYYLCSERSMRFVHGILYAYFA
                     FFALWWIFPWAIVTVRSRSWLTR"
BASE COUNT          281 a          476 c          463 g          219 t
ORIGIN      
        1 gtctggggcc cgggccagcg caccccgatc cacgatcatt gcacctgggg ggtgatcggc
       61 atgctgcggg gtgaggagat cggccgaccc tatcgcctgg aacgcgggcg tgtcgttccg
      121 gacggccccg aggagcgact cgtgcccggc cagacgatga gagtgtcacc gtccatcggc
      181 gatatccata cggttcgcaa cgcgttcgac gaccgcacct cgatcagcat tcacgtatac
      241 ggcgcggaca tcggcaagca gaagcgccat gtctacgatg cgacgagcgc cacggtgaag
      301 atgttcgtgt cgggctactc gaaccatccc gactatggca aatagggaag ctctgcggag
      361 tttcctatcg ggtgagccac gaacgtgagc ggacggtcac gatcgcccac gggaagatcc
      421 accacagcgc gaagaacgcg aagtaggcgt agagaatgcc gtgcacgaag cgcatcgagc
      481 gctcggagca gaggtaatag agcatcgaga acgcggcacc gatgccgatc gcgagcagca
      541 ccttgagggc gatcgccggg tcggccgcga ttgcggcggc gagaaccgcg agcccgatcc
      601 agccgatcgg aacgccgacc ccgaacatcg tcttttcgag cgccgcgaag agcatcgatg
      661 cgagcgggcg cttccatacg atccgcagga agcgcaattc ctcacgcaca tggctgcgat
      721 cccagcgcag gtacatcctg cacagtcccg cgtaggtcgt cggcaccagc gtttgcacga
      781 cggcgttgct ctggtagacg gtgtcgaagc cacacgccat gatgtcgttg gtcagcgcgc
      841 ggtcctcgcc gaacgtgcat cgtgcaccga ggaagcgttg cgccagccac ggctcgagga
      901 cctgctccac gacgctcgcc cgataggcgg cgagcgcgcc cgggcaacag tagacggtcc
      961 gacacgtcga ctgcacgctg cgctggacgt cgaacgagag aatgaatcgc acgtgcagca
     1021 tccgcgagat cacaccgcga tcgcgattcc aagccgcgac cttgccggcg acggcgccga
     1081 tccgcggatc gcggaactgt cccgccatcg cgaggagtgc gtcgcgctcg atcatgctgt
     1141 ccgaatcgac cgtcaccagg atcgcgccgc gggcaatgcg aaatcccgca gcgagcgcct
     1201 cgcgcttgcc gcggtttcgt gccagcctca acgtcgtcac ccgacccgaa tggcggaccg
     1261 cagcgcgcat gatgtactcc caggtgtcgt cgttgctccc gtcgtcgacg acgacgatct
     1321 gcaggcgctc cgcaggataa cgggcaccgg cgatcgaatc gatcgcccgc tcgaccgtcg
     1381 gaccttcgtt gtacgccggg atcacgaccg tcatcgtcgg cgccgcgtcg aattcgacc
//
LOCUS       DASUCJ010000765         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_390176, whole genome shotgun sequence.
ACCESSION   DASUCJ010000765 DASUCJ010000000
VERSION     DASUCJ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390176"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1614
                     /locus_tag="VFU90_09175"
     CDS             <1..>1614
                     /locus_tag="VFU90_09175"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5479991.1"
                     /translation="KHGQTIQLIAKNVSYTATAGTPEAFAVAAGFSDSLLATAPIASQ
                     PKPGTKSILVDANALLFADIPGASTVLERTYRQSYSFDARNSSFGKLWTSPDNVALEV
                     SAHYALAHIALPPPPPARSTLPRPPSTLPDIRSLFLGYYYTFATLPAEPMHPRLADDR
                     IGYFTTDRFDFTSDLPRLPIEHFIDRWRLEKKDPSAPISEPKHQIVYWLDRDIPVKYR
                     EPIREGILEWNKAFEKIGFKDAIRVEVQPDNADFDTSDIRHASVRWQTVAQSEYGAIG
                     PSVVDPRTGEILDADIGIDANNIRNVRNLRREYLPPASDAYRALAGPLWTLPGAARHG
                     WGSGFSAAGNEPMCSYASAATEEAAFGLSLLELRGEIAPDSADVDRFVDAFLKAVVMH
                     EVGHTLGLRHNFRASTIYTEAELSDPAFTRTHGIAGSVMEYNPWNIAAAGERQGAYDM
                     TTLGPYDYWAIEYGYREIPAADEAKTLAAVAGRSNEHDLAFASDEDVFMFGIDPAVNV
                     LDLGDDPLAYAKKRLELVHEVWQRTEALKL"
BASE COUNT          288 a          566 c          503 g          257 t
ORIGIN      
        1 aagcacgggc agacgatcca gttgatcgcg aagaacgtca gttacacggc gaccgccggt
       61 acgcccgaag cattcgcggt cgcagcgggg ttctcggaca gcctcctcgc caccgcgccg
      121 atcgcgtcgc agccgaagcc cggcacgaag tcgattctcg tcgacgcgaa cgcgcttctc
      181 tttgccgaca ttccgggcgc atcgacggtg ctcgagcgca cctatcgcca gtcgtactcg
      241 ttcgacgcgc gcaattcgtc gttcggaaag ctgtggacga gcccggacaa tgtcgcgctc
      301 gaagtctccg cgcattacgc gcttgcgcac atcgcgctgc cgccgcctcc gcccgcccga
      361 tcgacgctgc cgcgaccgcc gtcgacgctg ccggacatcc gcagtctctt ccttggttac
      421 tactacacgt tcgcaaccct cccggccgag ccgatgcatc cacgtcttgc ggacgaccgc
      481 atcggctact tcacgaccga ccgtttcgac ttcacctcgg acctgccgcg cctgccgatc
      541 gagcacttca tcgaccgatg gcgcctcgaa aagaaggatc cgtcggcgcc gatctccgag
      601 ccgaagcacc agatcgtgta ctggctcgac cgcgacattc cggtcaagta ccgcgagccg
      661 atccgcgagg ggatcctcga gtggaacaag gcattcgaga agatcgggtt caaggacgcg
      721 atccgcgtcg aagtgcaacc ggacaatgcc gacttcgaca cctcggacat ccgccacgca
      781 tcggtgcgct ggcagactgt cgcgcaaagc gagtacggtg cgatcggccc gtccgtcgtc
      841 gacccgcgca ccggcgagat cctcgatgcc gacatcggca tcgatgcgaa caacatccgc
      901 aacgtgcgga acctccgccg cgagtatctg cccccggcga gcgacgcgta ccgcgcgctc
      961 gcaggtccgc tgtggacgct gccgggcgcg gcccggcatg ggtggggctc cggcttctct
     1021 gccgcgggca atgaacccat gtgctcctac gcgagtgccg ccaccgagga agccgcgttc
     1081 ggattgtcgc tcctcgagct gcgcggtgag atcgcacccg acagcgccga cgtcgaccgc
     1141 ttcgtcgacg cgttcctgaa ggcggtggtg atgcatgagg tcgggcatac gctggggttg
     1201 cggcacaact tccgcgcgtc gacgatctac accgaagccg agctctccga tccggcgttc
     1261 acacgcacgc acgggatcgc cggctcggtg atggagtaca acccgtggaa catcgcggcg
     1321 gcgggcgagc gccagggcgc ctacgacatg acgacactcg gcccctacga ctactgggcg
     1381 atcgagtacg gttaccgcga gattcccgct gccgacgaag cgaagacgct ggcggcggtc
     1441 gccgggcgct ccaacgagca cgacctcgcg ttcgcgagtg acgaggacgt gttcatgttc
     1501 ggcatcgacc cggcagtcaa cgtcctcgat ctcggcgacg acccgctcgc ctacgcgaag
     1561 aagcgcctcg agctggtgca cgaagtgtgg cagcgcaccg aggcgctgaa gctc
//
LOCUS       DASUCJ010000766         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_756986, whole genome shotgun sequence.
ACCESSION   DASUCJ010000766 DASUCJ010000000
VERSION     DASUCJ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756986"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..>1047
                     /gene="ugpC"
                     /locus_tag="VFU90_09180"
     CDS             286..>1047
                     /gene="ugpC"
                     /locus_tag="VFU90_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5479992.1"
                     /translation="MASVVVNDVRKSFGTTAVLHGVNVTIEDGEFVVLVGPSGCGKST
                     LLRMIAGLENITSGEISIGDRVVNNLPPKARDVAMVFQNYALYPHMTVAANMAFSMKL
                     RGAAQDETDARVKRAAEILGLSNLLERFPRQLSGGQRQRVAMGRAIVRDPKVFLFDEP
                     LSNLDAALRGQTRVEIAKLHRELGATTIYVTHDQVEAMTMADKIVVLRDGNVMQVGAP
                     LDLYNAPANQFVAGFIGSPKMNFLPARLAGTGDAIE"
BASE COUNT          197 a          353 c          320 g          177 t
ORIGIN      
        1 cggtccggtt gcttcccggc gggcgcagag acggaacgag gtcctcggct gctaggccgc
       61 gcaggatttg caattccttc gcgcttgaac tctgacaacg gccctgcgtc ggcctcccgc
      121 cgcatcaaaa gcaaccagct accgcaccgc gcgcaaaagc cgcgaatttc ttcgcgcgaa
      181 acccctatga ttgcgcaaat tcgccgctac aatgcgccgc gtcgggtctt tgactcggca
      241 cgaataagaa taacgaagtc aaagcgttag ccccagggag ggttcatggc gtccgtcgtc
      301 gtcaacgacg tgcgcaagtc gttcggaacg acagccgtgc ttcacggcgt caatgtcacc
      361 atcgaagacg gcgaattcgt cgtgctcgtc ggtccgtccg gctgcggcaa gtctacactt
      421 ctgcgcatga tcgcggggct ggagaacatc acctccggcg agatcagcat cggcgatcgc
      481 gtcgtcaaca atctgccgcc caaggcgcgc gatgtggcga tggtctttca gaactacgcg
      541 ctctatccgc acatgaccgt cgccgcgaac atggcgttct ccatgaagct gcgcggcgcg
      601 gcgcaggacg agaccgacgc gcgcgtcaag cgcgccgccg aaatcctcgg cctgtcgaac
      661 ctgctcgagc ggttcccgcg gcaactctcc ggcggccagc gtcagcgcgt cgccatgggc
      721 cgcgccatcg tgcgcgaccc aaaagtgttc ctgttcgacg agccgctgtc caacctggac
      781 gccgcgctgc gcggccagac ccgggtggag atcgccaagc tgcaccgcga actgggcgcg
      841 actacgatct atgtcacgca cgaccaggtc gaggcgatga ccatggcgga caagatcgtg
      901 gtgctccgcg acgggaacgt catgcaggtc ggcgccccgc tcgatctcta caatgcgcct
      961 gcgaaccagt tcgtcgccgg cttcatcgga tcgcccaaga tgaactttct cccggcgcgc
     1021 ctggccggaa cgggggacgc catcgag
//
LOCUS       DASUCJ010000767         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1444910, whole genome shotgun sequence.
ACCESSION   DASUCJ010000767 DASUCJ010000000
VERSION     DASUCJ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444910"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFU90_09185"
     CDS             complement(<1..298)
                     /locus_tag="VFU90_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479993.1"
                     /translation="MRGRFAPLVLAIVAAALPGRAPALPIFAHRYGLSCQSCHTTVPT
                     LNAFGRYFLRHGFRLPGGRGTFPLTVKTQLTYTSGSNETDGLPLPKFIVDEIELL"
     gene            399..812
                     /locus_tag="VFU90_09190"
     CDS             399..812
                     /locus_tag="VFU90_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5479994.1"
                     /translation="MQVRPALPQELAVLERMPHALHYSHHPEFIRHALGTKRCLVAEI
                     DGAPAGFAVWDRGFYARPFLWMLGVDPAHQHRGVASELLEHVERLNAGLPLYTSTNES
                     NDAMHALLTSRGYAPTGRLDSLDPGDPEIFFVKRL"
     gene            complement(816..>1530)
                     /locus_tag="VFU90_09195"
     CDS             complement(816..>1530)
                     /locus_tag="VFU90_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5479995.1"
                     /translation="HLKNSDDGDLGLTQILGHKGDLMLTHYARGFEGLAYAQTLVDKL
                     ELREFLQPLGSYVSVLELGMYGATAKIHAQLESRGLPPQSPEWIAAFDELLREQEESP
                     YVGPRLWAKIPPRRYACFYPMNKRRGESVNWYALGFDERAKMMSEHGVVGRSYHGQVT
                     QVISGSIGFDDYEWGVDLYADDPLVFKKLIYEMRFDEASARYAEFGPFWAGLQFSPSE
                     LSVFLNGDAVPQLIAKSRK"
BASE COUNT          283 a          494 c          479 g          274 t
ORIGIN      
        1 acagcagctc gatttcgtcg acgatgaatt tgggcagcgg caagccgtcg gtttcgttgg
       61 agccgctcgt gtaggtgagc tgcgtcttga cggtcagggg gaaggttccg cggccgcccg
      121 gcagccggaa gccgtggcgc aggaaatagc ggccgaacgc gttgagggtc ggcacggtcg
      181 tgtggcaaga ctggcacgac agcccgtagc ggtgcgcgaa aatcggcagc gcgggggccc
      241 gcccgggcaa ggcagccgcc acgatcgcga ggaccagcgg agcgaaacgg ccgcgcaaaa
      301 aggttctcct aaatttatag ttaggtcaac ctaaattacg aagactatcc cggctctccc
      361 ttcaaggggg ctcgcccggc ggcgtcgcac ggccagggat gcaagttcgt cccgcacttc
      421 cccaagagct ggccgtgctc gagcgcatgc cgcacgcgct gcactacagc caccatcccg
      481 agttcattcg tcacgcgctc ggtaccaaac ggtgcctggt tgcggagatc gacggcgcgc
      541 cggccggttt cgcggtgtgg gaccgcgggt tctacgcgcg gccgtttctg tggatgctcg
      601 gcgtcgaccc ggcgcaccag catcgcgggg tcgcttccga actgctcgaa cacgtcgagc
      661 gtcttaacgc ggggctcccg ctttacacgt ccaccaacga gtccaacgat gcgatgcacg
      721 cgctgctgac gtcgcggggc tacgcgccga ccggacgtct cgacagtctc gatcccggcg
      781 atccggagat cttcttcgtc aaacgcctgt aggcgctact tacgggactt agcgatcagc
      841 tgaggaacgg cgtcgccgtt gagaaagacc gacagctccg acggcgagaa ttgcagcccc
      901 gcccagaacg gaccgaattc cgcgtaacgc gcgctggctt cgtcgaagcg catttcgtag
      961 atgagttttt tgaagaccag cggatcgtcg gcgtacagat cgacccccca ttcgtagtcg
     1021 tcgaacccga tcgagcccga gatcacttgc gtcacctgcc cgtggtacga gcgccccacc
     1081 accccgtgtt cgctcatcat tttcgcccgc tcgtcgaatc cgagcgcata ccagttcacg
     1141 ctttcgccgc gacgtttgtt catcggataa aagcacgcat agcggcgcgg cgggatcttc
     1201 gcccacagcc gcggaccgac gtacgggctc tcctcctgct cgcgcaacag ctcgtcgaag
     1261 gcggcgatcc attccggcga ttgcggcgga aggccgcgcg actccaactg tgcgtgaatc
     1321 ttggcggtcg cgccgtacat tccgagctcg agcaccgaaa cgtacgagcc caacggctgc
     1381 agaaactcgc gcagctctag tttgtcgacc aaggtctgcg cgtacgccag gccttcgaag
     1441 ccgcgcgcgt agtgcgtgag catcaagtcg cccttgtggc cgaggatttg cgtcagaccc
     1501 agatcgccat cgtcggaatt cttgagatgg
//
LOCUS       DASUCJ010000768         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_404999, whole genome shotgun sequence.
ACCESSION   DASUCJ010000768 DASUCJ010000000
VERSION     DASUCJ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404999"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFU90_09200"
     CDS             complement(<1..668)
                     /locus_tag="VFU90_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479996.1"
                     /translation="MMLVAEGHALAQPTPSSPASDPPPAPSGAADTAPDANDAAGSKP
                     VDSPDATGQTPPPAPTEDAEWVQRDRDINEAATLTGGSGLLRTQHAETGAAGQFRLGF
                     VGEWFSAGFLCSPQFQCPNPRGTGPSLSTDTMNHIGGTLTLGVSIAKLGAGTLEGYAA
                     TVAYANSDNADRTPLLQVLGDTDLGVKYMAPVGDVVRLGLFTELMLVNGLGSVGLDDS
                     GTSAR"
     gene            944..>1500
                     /locus_tag="VFU90_09205"
     CDS             944..>1500
                     /locus_tag="VFU90_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5479997.1"
                     /translation="MAQRGRILIVDDESNARAALSEILREEGYATETAADGFKALGKL
                     DEFGPDVILTDLKMPGLDGIAFMEKARVACPGASFVVMTAFGTISSAVAAMKKGAENY
                     LLKPLDPEALGAVVERAMEKASLVQEAQRLRDRLRERNAFSHIVSSDAKMEAVLELVS
                     RVGPSRASVLITGESGTGKELIAEA"
BASE COUNT          280 a          487 c          491 g          242 t
ORIGIN      
        1 cgcgcgctcg taccgctgtc gtccagcccg acggagccaa ggccgttgac gagcatcagc
       61 tccgtgaaaa gccccaggcg aacgacatcg cccacggggg ccatgtactt cacaccgagg
      121 tccgtatcgc cgagcacctg aaggaggggc gtgcgatcgg cgttgtcgct attcgcgtac
      181 gccaccgtcg ccgcgtagcc ttcgagagtg ccggcgccga gcttggcgat cgaaacgccg
      241 agcgtcagcg tcccgccgat atggttcatc gtgtccgtcg acaaactcgg ccccgttccc
      301 cggggattcg gacactgaaa ctggggtgag caaagaaacc ccgccgagaa ccactcgccc
      361 acgaagccaa gacgaaattg ccccgccgcg cctgtctcgg cgtgctgcgt gcgcaagagc
      421 ccggacccgc cggtcagcgt cgccgcttcg ttgatgtctc gatctcgctg cacccactcg
      481 gcgtcttcgg tcggcgcggg cggcggcgtt tgtcccgtcg catccggaga atcgacgggc
      541 ttgctaccgg cagcgtcgtt tgcatcgggc gccgtgtcgg ccgcgccgga tggcgcgggc
      601 ggcgggtcgc tcgccggaga gctcggcgtg ggctgcgcca aggcatggcc ttcagcaacg
      661 agcatcatga aggccacggc gtgcggtgca gcggcccatt tcgaatgaag tgcgcgggag
      721 cgtgtcattg gcactctcaa gatgaccagc gtctctgcca cccgtgacga aaaccgtgcc
      781 gacaccggcg ccgaaaagcg cgccgtaaaa ggcaaacggg cggcttgcaa gacaacaatg
      841 ggtgcggccg aacggtttct accatcaaag tgaggccgta ctccaaaaag gctgtcgtcc
      901 ctgtcgattt gccggcgcgt ccgccgggca ttaacgtcgc gtgatggcac aacgtggccg
      961 catcctcatc gtcgacgacg agtcgaatgc acgcgccgcc ctcagtgaga ttctgcgcga
     1021 agaaggttac gccacggaga cggccgccga cggattcaag gcgctcggaa agctcgacga
     1081 gttcggaccg gacgtcattt tgaccgacct caagatgccc ggcctcgacg ggatcgcgtt
     1141 catggagaaa gcgcgggtcg catgccccgg cgcgtctttt gtcgtgatga cggcattcgg
     1201 cacgatttcg agcgccgtcg ccgccatgaa gaaaggcgcc gagaactacc tcctcaagcc
     1261 gctcgacccc gaggcgctcg gcgcggtcgt cgaacgtgcg atggagaaag cttcgctcgt
     1321 gcaagaagcg cagaggcttc gcgaccgcct tcgggagcgc aatgccttca gtcacatcgt
     1381 gtcgagcgac gccaagatgg aggccgtcct cgagctcgtg tcacgggtcg gcccgagccg
     1441 cgcgagcgtt ctcatcacgg gcgaaagtgg aacgggcaaa gagctcatcg ccgaggcgat
//
LOCUS       DASUCJ010000769         1413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1215786, whole genome shotgun sequence.
ACCESSION   DASUCJ010000769 DASUCJ010000000
VERSION     DASUCJ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215786"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFU90_09210"
     CDS             complement(<1..199)
                     /locus_tag="VFU90_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5479998.1"
                     /translation="MTTHHHAASVFRRSIRWASVLALSIAALAWRPTARPSYLFMWAG
                     DSAHKASDFLAVIDATPTSPRY"
     gene            complement(300..710)
                     /locus_tag="VFU90_09215"
     CDS             complement(300..710)
                     /locus_tag="VFU90_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5479999.1"
                     /translation="MIATAKRTTPNVVDSSAWLAYFADEPGAAHFAGAIEAVDRLIVP
                     AVCLLEVFKVVLRQRGESDALQAVALMQQGTVVDLDATLAIAAASAGVAHKLPLADSI
                     VYATAQAAGAVVWTQDDDFDGLPGAEYYPRARAK"
     gene            complement(707..913)
                     /locus_tag="VFU90_09220"
     CDS             complement(707..913)
                     /locus_tag="VFU90_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5480000.1"
                     /translation="MDTVTISPKFQVVIPREIRERLGLEAGQQVRALAYENRIEFIPV
                     RPLKRMRGFLRGIDTAVRRDRDRV"
     gene            complement(1015..>1413)
                     /locus_tag="VFU90_09225"
     CDS             complement(1015..>1413)
                     /locus_tag="VFU90_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480001.1"
                     /translation="SGTSPEGILGREHPHPRAYATFPRILRKYVHDEHLLTLPDAIRK
                     FTALAAQREHLVDRGVIKRGMWADLVVFDPDSVRDVATYEKPNQLSVGMRWVLVNGVP
                     VIADGRMTGAKPGRILYGPGYRGAAPAAAR"
BASE COUNT          242 a          482 c          474 g          215 t
ORIGIN      
        1 cgtaccgtgg tgacgtcggc gtcgcatcga tcaccgcgag gaagtcgctg gctttgtgcg
       61 ccgagtcgcc ggcccacatg aagaggtagg acggacgcgc cgttgggcgc cacgcgagcg
      121 ccgcgatcga gagcgccaga acggatgccc aacgaatcga gcgccggaac acactcgcag
      181 catggtggtg cgtggtcatc gaatttgggg gaaagagacg gtggcgcggc gtccgcgggg
      241 gtcgcaaaaa gtatggccgc gcggcggacg tcgccagcgt tgccgagcgc gcgcagccgc
      301 tatttcgccc gcgctctcgg gtagtactcg gcgcccggaa gcccgtcgaa gtcgtcgtcc
      361 tgcgtccaga cgaccgcgcc agccgcctgt gcggtggcgt acacgatgct gtcggccagt
      421 ggcagcttat gcgcgacgcc tgccgacgcc gccgcgatgg ccagcgttgc gtcgaggtcg
      481 acaactgtgc cctgctgcat gagggcgacc gcctggaggg cgtcgctttc gccgcgctgc
      541 cgcagcacga ccttgaagac ctcgagcaga catacggccg gcacgatcag gcggtcgacg
      601 gcctcgatgg cgcccgcgaa gtgggcagcg cccggctcgt cggcgaaata ggcgagccag
      661 gcggatgagt cgaccacgtt gggcgtggtc cgcttggccg tcgcgatcac acgcgatcgc
      721 ggtcgcggcg caccgcggta tcgatcccgc gcaggaagcc gcgcatgcgc ttgagcggcc
      781 gcaccgggat gaactcgatc cggttttcat aggcaagggc gcgcacctgc tggccggcct
      841 ccagaccgag ccgctcccgg atctcccgcg gaatgaccac ctggaatttg ggcgagatcg
      901 tgacggtgtc caccggaacc tcgatcgata gagatttcgt actacggtag ctcaatctat
      961 cgacgattcg acatacctgc aaggaacgtt cgacacgcgg ttgcaccaac acgcctaacg
     1021 cgccgctgcc ggcgccgcac cccgataccc ggggccgtac aggatccgcc ccggcttcgc
     1081 accggtcatc cgcccatcgg cgatcaccgg cacgccgttc accagcaccc agcgcatccc
     1141 caccgacagc tgattgggct tctcgtacgt cgcgacatcc cgcaccgagt cgggatcgaa
     1201 caccacgaga tcggcccaca tcccgcgctt gatcacgccg cgatccacga gatgctcccg
     1261 ctgcgccgcc agcgccgtga acttccggat cgcatcgggc agcgtcaaca ggtgctcgtc
     1321 gtgcacgtac ttgcggagta tgcgcgggaa cgtcgcgtac gcgcgcgggt ggggatgttc
     1381 ccggccgaga atgccttcgg gcgacgtgcc gga
//
LOCUS       DASUCJ010000770         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_772426, whole genome shotgun sequence.
ACCESSION   DASUCJ010000770 DASUCJ010000000
VERSION     DASUCJ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772426"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1910
                     /locus_tag="VFU90_09230"
     CDS             <1..>1910
                     /locus_tag="VFU90_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480002.1"
                     /translation="SKSGYESATTTGVSVFADQAQSLRISLGPALKTIATVTTRSSMD
                     VVKPGTTTDVYSVNAAVTQAVQGVGGGGNLNNAYSAIATLPGAFVPPNQQGWDQVVYI
                     RGGNFDQIGYEFDGVPVNRSFDNYPGGTAGTLGQQELQVYAGGGTAGQSASGLAGFIN
                     QVIKTGTYPAYANVAFGLGTPTYYHNLQVEVGGASPDRLFSYYAGIGGYNQDYRYLDQ
                     FNGSNLGDVWGYPIIAYNTTFLYFGGVYPECGYTPRTGSGYYAGPNASPVYNPFDLRP
                     GQPGYLKLPAGVTNNPGCYQTISPAYASYSGISDRESVANLHFGIPHRRDSGHDDVQL
                     LYNITSMNSQYYSSANDVGPHLIAQLNQLATGKAIPEYWGDFVTWPGGTQFGENPAKV
                     APVPYFMPSSPGDRCANVTPTRAAGLPPIIPGACPGGVYSAVPQDARDAFWNDASIAK
                     LQYQRNIGSNAYLRVYGYTFYSDWLQTSPLSFASGLFGFGVTSYDYELESHTRGLAFS
                     FADQIDSKNLVTFDANYTTATTNRYNNTTFNNFLYTPATSLTNGAECFNYLTHAVAPC
                     NLPSTSGTFYRPEPAPAFPGARWEITYTGNSGFVNNVVPNFTSVALQETWSPSDKLIV
                     NLGLRDEVYEYNL"
BASE COUNT          373 a          692 c          514 g          331 t
ORIGIN      
        1 tcgaagagcg gttacgagag cgcgacgacg accggcgtct cggtgttcgc cgatcaggcg
       61 caaagcttgc gcatcagttt gggccccgcc ctcaagacga tcgccaccgt caccacgcgc
      121 tcctcgatgg acgtcgtcaa gcccggcacc acgaccgacg tctattcggt caacgcggcg
      181 gtcacgcaag cggtgcaagg cgtcggcggc ggcggaaatc tcaacaacgc gtactccgcg
      241 atcgcgacgc tgcccggcgc gttcgtcccg cccaaccagc aaggctggga tcaggtcgtc
      301 tacatccgcg gcgggaattt cgatcaaatc ggttacgaat tcgacggtgt cccggtcaac
      361 cgttcgttcg acaactatcc cggcggaacg gccggaacgc tcggccagca agagctgcaa
      421 gtgtatgccg gcggcggcac ggcgggacag agcgcgagcg gcctggccgg cttcatcaac
      481 caagtcatca agaccggaac ctatcccgcg tacgccaacg tcgcgttcgg cctcggaacg
      541 cccacctact atcacaacct gcaggtcgaa gtcggcggcg cttcgcccga ccggctgttt
      601 tcctattacg ccggcatcgg cggctacaat caagactacc gttatctcga tcagttcaac
      661 ggctcgaacc tcggcgacgt ctggggctat ccgattatcg cctacaacac cacgttttta
      721 tatttcggcg gcgtctatcc ggagtgcggg tacacaccgc gcaccggttc cggctactac
      781 gccggcccca acgcctcgcc ggtctacaat ccgttcgatc tgcgccccgg ccagccggga
      841 tacctgaaac tcccggccgg cgtcaccaac aatcccggct gctaccaaac gatctcgccg
      901 gcctacgcga gctactccgg catcagcgat cgcgagagcg tcgccaacct gcacttcggc
      961 atcccgcacc gccgcgactc cgggcacgac gacgtgcagc tgctctacaa catcacctcg
     1021 atgaacagcc agtactacag ttccgccaac gacgtcggcc cgcacttgat cgcgcagctc
     1081 aaccagctcg cgaccggcaa agcgattccg gagtactggg gcgatttcgt cacctggccg
     1141 ggcggtacgc agttcggcga gaatcccgcg aaggtcgcgc cggttccgta tttcatgccg
     1201 agctcgcccg gcgaccgctg cgccaacgtc acgcccacgc gagccgccgg actgccgccg
     1261 atcatcccgg gagcctgtcc cggcggcgtc tactccgccg tcccgcaaga cgcccgcgac
     1321 gccttctgga acgacgcctc gatcgccaag ctccaatacc agcgcaacat cggctcgaac
     1381 gcctatctcc gcgtctatgg ctacacgttt tactccgatt ggctgcaaac cagcccgttg
     1441 agtttcgctt ccggcctctt cggcttcggc gtcaccagct acgactacga gctcgagtcg
     1501 cacacgcgcg gtctggcgtt cagttttgcc gatcaaatcg actcgaagaa cttggtgacc
     1561 ttcgacgcca actacacgac cgcgacgacc aaccgctaca acaacaccac cttcaacaac
     1621 ttcctctata cgccggcgac cagcctcacc aacggtgcgg agtgcttcaa ctatctgacc
     1681 cacgcggtcg cgccgtgcaa cttgccctcg accagcggca ccttctaccg tcccgagccc
     1741 gcgccggcgt tccccggcgc gcgctgggaa atcacctaca ccggcaactc cggtttcgtc
     1801 aacaacgtcg tcccgaattt cacctcggtc gcgctgcaag aaacctggag tcccagcgac
     1861 aagctgatcg tcaatctggg gttgcgcgac gaagtttacg aatacaatct
//
LOCUS       DASUCJ010000771         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_69567, whole genome shotgun sequence.
ACCESSION   DASUCJ010000771 DASUCJ010000000
VERSION     DASUCJ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69567"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFU90_09235"
     CDS             <1..852
                     /locus_tag="VFU90_09235"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEU5480003.1"
                     /translation="RLPQYRGDGLARIRQSVLVDQPVDSASERMQLVSFLKSVQQALP
                     PNDPYRSIVLGLRAPEDVAASLLSLTKLASLSARQALLDGGKAAVDTSTDPMIVLARK
                     IAPLVDQVQEAEARLNDVISANADRVGQALFAVYNTKLPPDATFTLRITDGVVKGYPY
                     NGTIAPYKTTFYGMFGHATAFDDKPPFHLPDRWMDQRAKMDLSTPLDFVSTNDIIGGN
                     SGSPVINRDGELVGLIFDGNIESLPNRFIFTDDAARSVSVSSRAIIEALRNVYESARL
                     ADELEGK"
     gene            959..>1506
                     /locus_tag="VFU90_09240"
     CDS             959..>1506
                     /locus_tag="VFU90_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5480004.1"
                     /translation="MAQPIAPVVTEGRSPAAPVVTTPAPRWVEFVLLCGVAAVTFGVT
                     RAAAEWRAPLSPGFRISLSPAKLPYYAALSTLRMVLAYIVSLVFSLAYARFAASSKAA
                     ERVMLPLLDILQSIPILSFMPGVVLGLVALFPGRTLGLELAAVILIFTSQAWNLAFSF
                     HQSLITIPSELTEAARIYRLGF"
BASE COUNT          221 a          515 c          481 g          289 t
ORIGIN      
        1 cgcctgccgc aataccgcgg cgacggcctg gcgcggatcc ggcagtcggt gctcgtcgac
       61 cagccggtcg attcggcgtc ggagcgaatg cagctcgttt cgttcctcaa gtcggtgcag
      121 caggcgctcc cgcctaacga tccctaccgg agcatcgtgt tggggctgcg cgccccggag
      181 gatgtggcgg cgtcgctgct ctcgctcacc aagctggcgt cgctgtcggc gcggcaggcg
      241 ctgctcgacg gcggcaaggc agcggtggac acgtccaccg atccgatgat cgtcctggct
      301 cgcaagatcg ctccgttagt cgaccaggtt caagaggcgg aagcgcgtct caacgacgtc
      361 atctcggcca acgccgatcg cgtcggtcaa gcgctgttcg cggtgtacaa caccaagctg
      421 ccgcccgatg cgacgttcac gctgcgcatc accgacggtg tcgtgaaggg ctatccgtac
      481 aacgggacca tcgcgccgta caagaccacg ttttacggca tgttcggtca tgcgacggcg
      541 ttcgacgaca agccgccgtt tcacctgccc gaccgctgga tggaccagcg ggcgaaaatg
      601 gatctgagca cgccgctcga cttcgtgtcg accaacgaca tcatcggcgg gaactcgggc
      661 agtcccgtga tcaaccgcga tggcgagctc gtgggtctca tcttcgacgg caacatcgag
      721 tccctgccta accgattcat tttcaccgac gacgccgcgc gctcggtgag cgtgtcgtcg
      781 cgcgccatca tcgaggcgct gcggaacgtc tacgagtcgg cgaggctggc ggacgagctg
      841 gaagggaagt agtcccatgc ggtgacacgt cgggcccggc gcgaccgttc gcgcggggga
      901 cacttccgtc tgtggcctgc gccgtttttc ttcctcgtct cccgaacccc gactcaggat
      961 ggcgcagccg atcgctcccg tcgttacaga gggccgctcg cccgccgcgc cggtcgtcac
     1021 gactccggcg ccgcggtggg tggagttcgt cctcctgtgc ggcgtggccg cggtcacgtt
     1081 cggcgtcacc cgcgcggccg ccgagtggcg agcgccgttg tcgcccggct ttcgcatctc
     1141 gctctctccg gccaagctgc cgtactatgc cgcgctctcc accctgcgca tggtgctggc
     1201 gtacatcgtg tcgctcgtgt tctcgctcgc atacgcgcgc tttgccgcgt cgagcaaggc
     1261 ggccgagcgc gtgatgctgc cgctgctcga cattctccag agcataccca ttctgtcgtt
     1321 catgccgggc gtagtgctcg ggctcgtcgc gctcttcccc ggacgaacgc tgggactcga
     1381 gctcgcggcc gtgattctca ttttcacgag ccaggcctgg aatctcgcct tcagctttca
     1441 ccagtcgctg atcacgattc cgagcgagct caccgaggcg gcgcgcatct atcggttggg
     1501 attctg
//
LOCUS       DASUCJ010000772         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_328746, whole genome shotgun sequence.
ACCESSION   DASUCJ010000772 DASUCJ010000000
VERSION     DASUCJ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328746"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFU90_09245"
     CDS             complement(<1..441)
                     /locus_tag="VFU90_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480005.1"
                     /translation="MQQNAFSKRRAPAWERLDALLLKAGRRGVRALDAAEVAELGRLY
                     RAATSDLAYARGREFDRRLTEYLNRLTARAHAYVYGGSAESGGERVARFYRESFPREF
                     RRSIVYVAICTALTVVSAVVAYALVRAHPAEAYALLPRSIVPDEI"
     gene            543..>1006
                     /locus_tag="VFU90_09250"
     CDS             543..>1006
                     /locus_tag="VFU90_09250"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU5480006.1"
                     /translation="MDRNLDVRTPESIALTYELAGLGSRFLAVTIDMILQVVVLVSLI
                     GGLALAASRIPAPAKHAVASSAEKIGEALGIAILAAIVFLIFFGYFILFEAFWNGQTP
                     GKKMLGIRVVRDGGYPVDFMASLVRNLIRVGELAIGSYALSAVAMLLSPENKR"
BASE COUNT          162 a          325 c          339 g          180 t
ORIGIN      
        1 gatttcgtcg gggacgatcg agcggggcaa aagcgcgtac gcctcggccg gatgcgcgcg
       61 gaccagcgcg tacgcgacga ccgcgctcac gaccgtgagc gccgtgcaga tcgcgacgta
      121 gacgatcgag cgccggaatt cgcgcgggaa gctctcgcgg taaaagcgcg cgacccgttc
      181 gccgccgctc tcggccgagc cgccgtagac gtaggcgtgc gcccgggccg tcaaccggtt
      241 caggtattcc gtcaagcggc gatcgaattc gcgtccgcgc gcgtaagcca gatcgctggt
      301 cgcggcgcgg tacagccgcc cgagttcggc aacctcggcg gcgtcgagcg cgcgcacccc
      361 gcgccggccg gccttcagca gcagcgcgtc gagccgctcc cacgccggcg cccggcgttt
      421 cgaaaacgcg ttctgctgca ccggcgatac tttcggcgtg cgtttgcgtt cctccgcgcc
      481 ttacgcgcga tagcttggaa aagtggaggc cgagcgccgc ggggcgaacc accgagccga
      541 cgatggatcg taatctcgac gtccgtacgc ccgaaagcat cgcccttacg tacgaattgg
      601 cggggctggg cagccgcttt ctcgccgtga cgatcgatat gatcctgcag gtcgtcgtgc
      661 tcgtgtcgct gatcggaggg ttggcgctgg ccgcgtcgcg gattccggcg ccggccaaac
      721 acgccgtcgc ttcgagcgcg gagaaaatcg gcgaggcgct cggcatcgcc atcttggcgg
      781 cgatcgtctt tctgatattc ttcggatatt ttattctatt cgaagcattt tggaacggac
      841 aaacgcccgg aaagaagatg ctcggcattc gcgtcgtgcg tgacggaggc tatccggtcg
      901 atttcatggc gtcgctggtg cgcaatctca tccgggtcgg cgagctggcg atcggctcgt
      961 acgccctctc ggcggtcgcg atgctgcttt cgccggaaaa caagcg
//
LOCUS       DASUCJ010000773         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1307084, whole genome shotgun sequence.
ACCESSION   DASUCJ010000773 DASUCJ010000000
VERSION     DASUCJ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307084"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1925)
                     /locus_tag="VFU90_09255"
     CDS             complement(63..1925)
                     /locus_tag="VFU90_09255"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480007.1"
                     /translation="MSLRTVLLSAAFAAAAFFISRPLLAAPAPVTLVVDATQASRGIL
                     YAHERIPVTPGTLTLVYPKWIPGEHGPTGPLNDLAALRMSADGKPLDWRRDQVDLYAF
                     HVNVPAGVQTLDVDFDVLLNAPDDEMATRSLAILNWNRALLYKDGVNSHDYFFKPSIE
                     LPDGWDYATALREPAKTGNRIDFAVTPLSMLVDSPLDLGRYVKKWDLWQAGSAFVQLD
                     AFADYPQDLDIPAALLKAYERMPAEAFAMYGSRHFLDYHALLTLSDAIGFQGIEHHQS
                     SDDRAPDDFLTDPEESTAGGDLITHEFSHSWNGKYRRPADLTTPNFQVPMRTDLLWVY
                     EGMNQYLGDVLSFRTGIREPQKYPEYLANVYAGMDTESGRATTPLIDLTTGAPYYYEA
                     RGNYGSIRRDANDFYTEGELVWLDVDTIIRARSHGERSLDTFLHRFTEPSTTGPITQT
                     YTRAQVERLLNEVEPYDWNAFFEKYVYQTSIHPPSDELERAGWRLVYTSKPNEFVQAE
                     GHQLTHWYSYGANISTKGTVRDVRPDSPAWKAGLVPDMTILAVNGQEFDPDVLDYALA
                     QAQHSMAPISLIVKQNGWYQTLSLNYHEGVRYPHLERIAGTPDMLSSIVAPHAK"
BASE COUNT          362 a          586 c          679 g          379 t
ORIGIN      
        1 ctccgctcag cccttcgact gcgcgtgctg cgcacgctcc gctcaggatg acacagcggg
       61 ttctatttgg cgtggggggc gacgatcgac gagagcatgt cgggggttcc ggcgatgcgc
      121 tcgagatgcg ggtagcggac gccctcgtga tagttcagcg aaagcgtttg ataccagccg
      181 ttttgcttga cgatcagcga aatcggcgcc atcgaatgct gcgcttgcgc gagcgcatag
      241 tcgagcacgt cgggatcgaa ctcttggccg ttcaccgcca ggatcgtcat atcgggaacg
      301 agcccggcct tccaggccgg cgagtcggga cgaacgtcgc gcacggtgcc tttggtcgag
      361 atgttggcgc cgtaggaata ccagtgcgtg agctggtggc cttcggcttg gacgaactcg
      421 ttcggcttgg acgtgtacac caagcgccag ccggcgcgct caagctcgtc ggacggcggg
      481 tggatcgacg tctgatagac gtacttctcg aagaacgcgt tccagtcgta gggctcgacc
      541 tcgttcaaca gccgctcgac ctgagcgcgc gtgtaggttt gcgtgatcgg gccggtcgtc
      601 gacggctcgg tgaagcggtg cagaaacgtg tcgagcgagc gttcgccgtg ggagcgcgcg
      661 cgaatgatcg tatcgacgtc gagccacacc agctcgcctt cggtgtagaa gtcgttggcg
      721 tcgcggcgaa tcgacccgta gttgccgcgc gcctcgtagt aatacggcgc gccggtggtc
      781 aagtcgatca gcggcgtggt cgcgcgtccc gattcggtat ccatgccggc gtagacgttc
      841 gccaggtact cgggatattt ttgcggctcg cgaatgccgg tgcggaacga cagcacgtcg
      901 cccaaatatt gattcatccc ctcgtacacc cacagcaagt cggtgcgcat cggcacttga
      961 aagttcggcg tcgtcagatc ggcgggccgg cggtatttgc cgttccacga gtgcgagaac
     1021 tcgtgcgtga tcagatcgcc gccggcggtc gattcctcgg gatcggtgag aaaatcgtcg
     1081 ggcgcgcggt cgtcgctgga ctgatggtgc tcgattcctt gaaaaccgat cgcatcgctg
     1141 agcgtcaaca atgcatggta atcgaggaaa tggcgcgagc cgtacatcgc aaacgcctcg
     1201 gcgggcatgc gttcgtaggc tttcagcaag gctgcgggga tgtcgagatc ttgcggatag
     1261 tcggcaaaag cgtcgagctg cacgaaagcg ctcccggctt gccacagatc ccatttcttc
     1321 acgtagcggc cgagatcgag cggcgagtcg accagcatgc tgagcggcgt gaccgcgaag
     1381 tcgatccggt tgccggtctt tgccggctcg cgtagcgcgg tggcgtaatc ccagccgtcg
     1441 ggcagctcga tcgacggctt gaagaagtag tcgtgggagt tgacgccgtc cttgtagagc
     1501 agggcgcgat tccagttgag gattgcgagc gaccgggtcg ccatctcgtc gtccggcgcg
     1561 ttgagcagca cgtcgaaatc gacgtcgagg gtctgtacgc cggccggaac gttcacgtga
     1621 aaggcataga ggtcgacttg atcgcgccgc cagtccagcg gtttcccgtc ggcagacatc
     1681 cgcaacgcgg cgaggtcgtt gagcggtccg gtcggcccgt gttcgccggg aatccacttc
     1741 ggatacacca gcgtcagcgt tccgggcgtc accggaatcc gctcgtgcgc gtacagaatg
     1801 ccgcgcgacg cctgggtggc gtccacgacg agcgtcaccg gagccggcgc ggccagcagc
     1861 gggcgagaaa taaaaaacgc tgcagccgcg aacgcagcgc tcaacaagac ggtacgaaga
     1921 ctcatgcgtt ttcgatttct tagcgcgggc ggacgaacct ttccccgaac tgcaaccagg
     1981 cgggcgccgg cgacggtttg atgagc
//
LOCUS       DASUCJ010000774         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_436301, whole genome shotgun sequence.
ACCESSION   DASUCJ010000774 DASUCJ010000000
VERSION     DASUCJ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..871)
                     /locus_tag="VFU90_09260"
     CDS             complement(116..871)
                     /locus_tag="VFU90_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480008.1"
                     /translation="MAKTIARLDDVSLAFGERVVLKNCNLDIVEGAITCIVGLSGAGK
                     STILRLLDGLIRPNTGHVYLRGQDICHMSEEQLIPARRKISLAFQFAALLDSLTIGDN
                     VALPLEERGDLSRAEIRQTVMDALESVGLENTYDNLPSELSGGMLKRAGFARAVVTRP
                     DLILYDEPTTGLDPIVTHLITNTIVKLREKLNGTAVVISHDLQSIFMMADYVAMLFEG
                     AIIAYDSVENIRRSPNPIVQQFLQGSEVGPIPI"
     gene            complement(874..1644)
                     /locus_tag="VFU90_09265"
     CDS             complement(874..1644)
                     /locus_tag="VFU90_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480009.1"
                     /translation="MLDRVGRATIEFFEYAGGITLLSAESLSFMARLRIRFAETIAQC
                     YLLGVQSLIIVLLTSLFTGAVISLESAQQAVQYGVGSLVGGAVAYASVRELGPMLTAV
                     VVAGRVGAAIAAEIGSMVVTEQIEALRSMGLEPSRFLVVPRLLAMLIMLPLLTIFADV
                     VSILGGMWIAQTYAHISVDTFLSSARQTIGFEDVLKGLVKSGVFACIIAMVGAYQGLK
                     TRGGAAGVGVSTTGAVVLSIILIFISNFVLSFLLFGGK"
     gene            complement(1647..1973)
                     /locus_tag="VFU90_09270"
     CDS             complement(1647..1973)
                     /locus_tag="VFU90_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480010.1"
                     /translation="MAALAERVAAFHITDRAMKRAQADIERALHGGTADARARAAYLA
                     AVRRYFTAFENEARTNLRDVDRRLVHVNQVHFNLTAERGVAVKRIEATEAILRDVAGL
                     QEHPVS"
     gene            complement(1981..>2277)
                     /locus_tag="VFU90_09275"
     CDS             complement(1981..>2277)
                     /locus_tag="VFU90_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480011.1"
                     /translation="RDAARWPLAGAVAAASVGIVNGQPMLDLAYDEDSQAHVDMNVFM
                     TDAGRFVEIQGTAEAAPFDRRDLDSLLSLAESGIRRLFDAQRDALNAAGTVHAG"
BASE COUNT          433 a          737 c          729 g          378 t
ORIGIN      
        1 caacagcaag gctataagag caaaggcgcc gacttgtgcc tgcttggtca tgttgaagct
       61 atcttaagcg tcctcgccgc agaggcctcg tgagggccca aaggacccac gccgctcaaa
      121 tcgggatcgg ccccacttca cttccctgta agaattgttg gacgatggga ttcggggaac
      181 ggcggatatt ttcgaccgaa tcgtaggcga tgatcgcgcc ttcgaagagc attgcgacgt
      241 agtccgccat catgaagatc gactgcaggt cgtgcgagat gacgaccgcc gtgccgttga
      301 gcttctcgcg cagcttcacg atcgtgttgg tgatcagatg cgtgacgatc gggtcgagtc
      361 cggtggtagg ctcgtcgtac agaatcaggt ccggccgcgt taccaccgcg cgcgcgaaac
      421 ccgcccgttt gagcatcccg cccgacagct cgctcgggag gttgtcgtag gtgttctcca
      481 gcccaacgct ctcgagcgcg tccattacgg tctggcggat ctcggcgcgc gaaagatcgc
      541 cgcgttcctc cagcgggagg gccacgttgt cgccgatcgt caggctatcg agcagcgctg
      601 cgaactgaaa ggccaggctg atcttgcgcc tcgccgggat cagctgttcc tcggacatat
      661 gacagatatc ctgaccgcgc aagtacacgt ggccggtgtt cggacgaatc aggccgtcga
      721 gcaggcgcag gatcgtcgac ttacccgcgc ccgacaatcc gacgatgcag gtaatcgcgc
      781 cctcgacgat gtcgaggttg cagttcttca agacgacccg ctcgccgaac gccagcgaaa
      841 cgtcgtccaa gcgggcgatg gtcttcgcca tcgctatttc ccaccgaaca gcaagaacga
      901 gagcacgaag ttcgagatga agatcaggat gatcgacagt acaaccgcgc cggtggtcga
      961 gacgccgacg ccggccgccc cgccgcgggt cttgagccct tgatacgcgc ccaccatcgc
     1021 gatgatgcac gcgaagacgc ccgacttgac caagcccttg agcacgtctt cgaatccgat
     1081 cgtctgccgc gccgacgaga ggaacgtgtc gaccgagatg tgagcgtacg tttgcgcgat
     1141 ccacatcccg cccaaaatcg agacgacgtc ggcaaaaatc gtcagcagcg gcagcatgat
     1201 cagcatcgcg agaaggcgcg ggacgaccag aaaacgcgaa ggctccaagc ccatcgaacg
     1261 caacgcttcg atctgctcgg tcaccaccat cgagccgatc tcggccgcaa tcgcggcccc
     1321 gacccgtccg gccaccacga cggcggtgag catcggcccg agctcgcgca ccgacgcgta
     1381 ggccaccgct ccgccgacca agctgccgac gccgtactga acggcctgct gcgcggactc
     1441 gagcgaaatc accgccccgg taaacaacga ggtaagcagg acgatgatca gcgactgcac
     1501 gccgagcagg tagcattgcg cgatcgtttc ggcaaagcgg atgcgcagcc gcgccatgaa
     1561 gctcagcgac tcggcggaca gcagcgtgat cccgccggcg tattcgaaaa attcgatcgt
     1621 cgcgcgaccg acgcgatcga gcatcctcac gatacggggt gctcttggag accggcaacg
     1681 tcgcggagga tcgcctcggt cgcctcgatg cgcttgaccg cgacgccgcg ttcggcggtg
     1741 aggttgaagt gcacttggtt gacgtgcacg aggcggcggt cgacgtcgcg caggttcgtc
     1801 cgggcttcgt tttcgaaggc ggtgaagtag cgccgcaccg ccgcgagata cgcggcgcgc
     1861 gcgcgcgcgt cggccgttcc gccgtggagc gcgcgttcga tgtcggcctg cgcgcgcttc
     1921 atcgcccggt cggtgatgtg gaacgcggcg acgcgttcgg ccagcgccgc caacggatcg
     1981 tcacccggca tgaaccgtgc cggctgcgtt caaagcgtcg cgctgcgcgt cgaaaagccg
     2041 gcggatgccg ctttcggcga ggctcagcag cgagtcgagg tcgcggcggt cgaacggcgc
     2101 cgcttcggcc gtgccttgaa tttccacgaa cctgccggcg tcggtcatga agacgttcat
     2161 atcgacgtgc gcctggctgt cctcgtcgta agcgaggtcg agcatcggct gcccgttgac
     2221 gatcccgacg ctggccgctg cgacggcgcc ggccagcggc caacgggcgg cgtcccg
//
LOCUS       DASUCJ010000775         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_359822, whole genome shotgun sequence.
ACCESSION   DASUCJ010000775 DASUCJ010000000
VERSION     DASUCJ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359822"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFU90_09280"
     CDS             <1..435
                     /locus_tag="VFU90_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEU5480012.1"
                     /translation="NPHMTTRERFDAHVADPICAGCHDIIDPFGFPFEHFDGMGQHRT
                     TENGKKVDSRVVVNGKADYDGPYADSNALATAFAASPTVRECMARFLFRASAATGDSA
                     ATPGERQFIDFWRTIPAAAQGNIVETLIAYVKSPSFGVREVP"
     gene            486..>1087
                     /locus_tag="VFU90_09285"
     CDS             486..>1087
                     /locus_tag="VFU90_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEU5480013.1"
                     /translation="MALPFVRVVEASHAQEAGVALPQRFIGMYHPHGIAAELFTRRDD
                     ESETTLELDYAHSPLQPFDDARTFGKSFKQQIITIEGLDLLSDTNGHNSAGTILTGSR
                     IVGGNGGRPENSSLDQFLAVEQKLGDSTLVTSVALGVGNGRASGAETLSFGRGGVPIS
                     KIIDPVLAFDFLFKQAVVGTDAVAQAAAERQRKLGLSLID"
BASE COUNT          192 a          359 c          353 g          183 t
ORIGIN      
        1 aatccgcaca tgacgacgcg cgagcgcttc gacgcgcacg tcgcggaccc gatctgcgcc
       61 ggctgtcacg acatcatcga cccgttcggg tttcccttcg agcatttcga cggcatgggc
      121 cagcaccgca ccaccgagaa cggcaagaaa gtcgacagcc gcgtcgtcgt caacggcaaa
      181 gccgactacg acggtccgta cgccgacagc aatgcgctcg ccacggcttt cgccgcaagt
      241 ccgacggtgc gcgagtgcat ggcgcgcttc ctgtttcgtg catccgctgc gacgggcgat
      301 tccgccgcga caccgggcga gcgccagttc atcgatttct ggcgcacgat ccccgccgcg
      361 gcccagggca acatcgtgga gacgctgatt gcctatgtga agagcccgag cttcggcgtg
      421 agggaggtac cttgagccac gagcaaagcc gtcgtcggtt cttgtgcggc ctaggcgcta
      481 cggccttggc gctgcccttc gtgcgcgtgg tggaggcgtc gcacgcgcaa gaggcgggcg
      541 tggcgctccc gcagcgcttc atcggcatgt atcaccccca cgggatcgcc gcggagctct
      601 tcacgcggcg tgacgacgag tcggagacga ccttggagct cgactacgct cacagcccgc
      661 tgcaaccgtt cgacgacgca aggacgttcg gcaagagctt caagcagcag atcatcacga
      721 tcgagggcct cgacctgctc tcggatacga atggccacaa ctcggcgggc acgatcctca
      781 ccggcagtcg catcgtcggc ggcaacggcg gcaggccgga gaacagctcc ttggatcaat
      841 tcctcgccgt ggagcaaaag ctcggcgatt cgacgctcgt cacgagcgta gctctcggcg
      901 tcggcaatgg ccgggcgtcc ggcgccgaga cgctgtcgtt cggcagggga ggggtgccga
      961 tctccaagat catcgatccc gtcttggcct tcgacttctt gttcaagcaa gcggtagtcg
     1021 gcaccgatgc ggtggcgcag gcggccgcgg agcggcagcg caagctcggc ctcagtttga
     1081 tcgattt
//
LOCUS       DASUCJ010000776         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_558505, whole genome shotgun sequence.
ACCESSION   DASUCJ010000776 DASUCJ010000000
VERSION     DASUCJ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558505"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFU90_09290"
     CDS             <1..1186
                     /locus_tag="VFU90_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5480014.1"
                     /translation="CDSMRRENPENRDAALLAAQTLAWSGRLDSATSRYDDWLARHPK
                     DADAWNALAQVWQWSGRSERARDALRHALDAEPANATALAQMQLTEVALAPSVEPAIT
                     STDDSDQNRTLTYLVRAGLVAPWSARVQTNVSFRTADLGLAHGTSTTVHASSSWTPLD
                     GAWTLAGGLGVAQLGGDDGSGTRTTHTEPIASARLSGRFASRMSLGVGVERGAFDETA
                     ALIYSGIANTTVDGDADIALRRNLSLGGGGGWARLTGGSAPNTRLAGSGALRWTLTRS
                     LSVAAGVRTFGYDHAAADGYFAPKRYLLAEGSARLHLGGDLGWGLDSELGLGHQRITA
                     FDASNAGRFAQRFNIAAAYRPVPGVEWSLGGSFANVASPTTISSADYRAYSVSLKGRL
                     RL"
     gene            complement(1164..>2147)
                     /locus_tag="VFU90_09295"
     CDS             complement(1164..>2147)
                     /locus_tag="VFU90_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480015.1"
                     /translation="GVIAMRPGRQDSLIVIDGRANDWAARRALYTGRPSPTLAPPLQL
                     RSFSVASDEAYVYMKLEVGAIDWRRAAYLIGIDTHSPALGDGVLPITGTRSPVGLEFV
                     VDLHGPDGSHVLVDHPYNLYREKPIRGSRPAAVQQVYNRPFRSVANSAGHYDSLVVET
                     NRRRIGRDGTVYPPRTVDRSRLLFARQSETTLADWYADTTTGVIELRIPWGMLNVLDP
                     SSRLVLRGDPRTGKVAGVRTDGFRFLVQSYDPRRPAAPGDRLPAGTAGGFGAVPTWRW
                     PTWEEPRWYPELKPQFDSMRAAFGRIPDRPASLTAAGAQALRTKLRGAADP"
BASE COUNT          346 a          738 c          729 g          334 t
ORIGIN      
        1 atgcgacagc atgcggcggg agaatcccga gaatcgcgat gccgcgctcc ttgccgccca
       61 gacgctcgcc tggagcggcc ggctcgactc tgccacctca cgctacgacg attggcttgc
      121 gcgccacccg aaggacgccg acgcctggaa tgcgctcgcc caggtgtggc aatggtcggg
      181 acgctccgaa cgcgcgcgcg atgccttacg ccacgcgctc gacgccgagc ccgccaacgc
      241 caccgcgctg gcgcagatgc aactgacgga agtcgcgctg gcaccctcgg tggagccggc
      301 catcacgagc accgacgata gcgaccagaa tcgcacgctc acgtacctcg tccgcgcggg
      361 gctcgtggca ccgtggagtg cgcgggtgca gaccaacgtg agtttccgca ccgccgatct
      421 cgggctcgcg catgggacat ccaccaccgt gcacgcgtcg tcgagttgga cgccactcga
      481 tggcgcctgg acgttggccg gtggactggg cgtggcgcaa ctcgggggcg acgacggttc
      541 gggaacacgt acgacgcaca cggagccgat cgccagcgcg cgtctctccg gtcgcttcgc
      601 gtcccgaatg tccctgggag tcggcgtgga gcgcggcgcc ttcgacgaga cggcagcgct
      661 catctattcc ggcatcgcga acacgaccgt ggatggcgat gccgacatcg cgctccgccg
      721 caatctctcg ttaggtggtg gcggcggctg ggcgcggctc accggcggct cggccccgaa
      781 cacccggctc gccggctccg gagcgttgcg gtggacactc accagaagcc tctccgtcgc
      841 ggcgggagtt cgcacgttcg gttatgacca tgccgcggcc gacgggtact tcgcgcccaa
      901 gcgctacctc ctagccgagg gcagcgcgcg attgcatctg ggcggtgatc tgggatgggg
      961 actcgattcg gagctgggcc tcggccatca acgcatcacc gcgttcgacg cttccaacgc
     1021 cggtcgattc gcgcagcgat tcaacatcgc tgccgcttat cggccggtac ccggggtcga
     1081 gtggagtctc ggcgggagct tcgcgaacgt cgcctcgccc accacgatca gctccgccga
     1141 ctaccgcgcg tacagcgtct cgctcaaggg tcggctgcgc ctctgagttt cgtccgcagc
     1201 gcctgcgcgc cggcagccgt taggctcgcc gggcgatcgg gtatgcggcc gaacgctgct
     1261 cgcatggaat cgaactgcgg tttgagctcg ggataccagc gcggctcttc ccacgtcggc
     1321 catctccagg ttggaaccgc accgaaaccg cccgcagtgc cggcggggag gcgatcgccc
     1381 ggcgcagctg gacgcctcgg atcgtagctc tggacgagga agcggaagcc gtcggtgcga
     1441 acgccggcaa ccttgccggt cctcgggtct ccacgcaaca ccagccgtga cgacggatcg
     1501 agcacgttca acatacccca cgggatgcgc agctcgatga cgccggtggt cgtatcggcg
     1561 taccagtcgg cgagtgtcgt ctcgctctgc cgcgcgaaga gaagccggct gcgatcgacg
     1621 gtccgcggcg gatacaccgt tccgtctcgc ccgatgcgcc ggcggttggt ctccacgacg
     1681 agtgaatcgt aatgacccgc ggagttcgcg accgaccgga atggccggtt gtaaacctgc
     1741 tgaaccgccg ccggcctgct gccgcgaatc ggcttctcgc gatacaggtt gtaggggtgg
     1801 tcgaccagga cgtggctccc atcggggccg tgcaggtcga cgacgaactc gaggccgacc
     1861 ggcgagcgcg tgccagtgat cggtagcacg ccatcgccca gggccggaga atgtgtatcg
     1921 atgccaatga ggtatgcggc tcgccgccag tcgatcgcgc cgacctcgag cttcatgtag
     1981 acgtatgcct cgtcgctcgc caccgaaaac gagcgcagtt gcagcggagg cgcgagcgtg
     2041 ggagacgggc gcccggtgta gagcgcgcgt cgcgccgccc agtcgttcgc gcgcccatcg
     2101 atgacgatca gagaatcctg gcggccgggc cgcatcgcaa tcacgcc
//
LOCUS       DASUCJ010000777         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_390313, whole genome shotgun sequence.
ACCESSION   DASUCJ010000777 DASUCJ010000000
VERSION     DASUCJ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390313"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>1298
                     /locus_tag="VFU90_09300"
     CDS             39..>1298
                     /locus_tag="VFU90_09300"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5480016.1"
                     /translation="MTSPAVSLRDPFAADKAARALSALLDLSKDLGPEFDLPKLLEVI
                     AEKARVSGEDEAVVGALEQYDRSLRLAGEIQMRMLPAATVALPDDSPFAIRAFIRPAK
                     QIGGDLYDFFWNDESLSFCIGDVTGKGVGAALVMAMTKTLLRAYSVFQYDPARLMSAV
                     SARLSEETDPSMFVTAFCGLLDLRTGRFLYSNAGHEPPIVIRGGKPVDRLTSRAGIPL
                     GALRSFKYVVDTRMLEPGDVVLLFTDGVTEAANRKGELFSFQRLREVVERSGAADPTS
                     IVTDIVVAVDQFAEGTLQSDDIAMVCVQFRGASREAAAKFRRDVSELQKIFDFIGDFF
                     AARGIHEAARRSVELAVEEIFMNYIRHNVAGKSDVEIRMTLDGEWLRIALTDFNAPAF
                     DVKRAPQPDVAQPLSKRRPGGLGIALAK"
BASE COUNT          226 a          417 c          406 g          249 t
ORIGIN      
        1 ccctgacata caatctgggc accctaaata acctcgcgat gaccagcccg gccgtctcac
       61 tccgcgaccc ctttgctgcc gacaaggcgg cgcgggccct ctccgcgctt ctcgatctct
      121 ccaaagacct cggtcccgaa ttcgacctgc cgaaattgct ggaagtgatc gctgaaaagg
      181 cgcgcgtgtc gggcgaagac gaggcggtcg tcggcgcgct cgaacagtac gaccgctcgc
      241 tccgtctggc cggcgagatc caaatgcgca tgctgcccgc ggccaccgtc gcgctgccgg
      301 acgattcgcc tttcgcgatc cgcgcgttca tccgcccggc gaaacagatc ggcggcgatc
      361 tctacgactt cttctggaac gacgagagcc tctcgttctg catcggcgac gtcaccggca
      421 aaggcgtcgg tgcggcgctg gtcatggcga tgaccaagac gctcctgcgc gcgtattccg
      481 tttttcagta cgacccggcg cggttgatgt ccgcggtcag cgcgcggctc agcgaggaaa
      541 ccgatccgtc gatgttcgtc accgcgttct gcggtctcct tgatctgcgc accggacgtt
      601 ttctttacag caatgccggg cacgagccgc cgatcgtcat tcgcggcggc aagccggtgg
      661 atcgcctcac atcgagagcc gggattccgc tcggcgcgtt gcgcagcttc aaatacgttg
      721 tcgatacgcg catgctcgag ccgggcgatg tcgtgctgct gttcaccgac ggagtgaccg
      781 aagcagcgaa tcgaaagggc gagctcttct cgttccagcg actgcgggag gtggtcgagc
      841 gcagcggtgc ggccgatccc acgtcgatcg tcactgacat cgtcgtcgcc gtcgatcaat
      901 tcgccgaggg gacgctgcag tcggatgaca ttgcgatggt gtgcgtgcag ttccgcggcg
      961 cctcacgtga agcggcggcg aagttccggc gcgacgtcag cgagctgcag aagatcttcg
     1021 acttcatcgg cgatttcttc gctgcccgcg ggatccatga agccgcacgg cgctcggtgg
     1081 agctggccgt cgaagaaata ttcatgaatt atattcgtca taatgttgca ggaaaatcgg
     1141 acgtcgagat ccgcatgacg ctcgacggcg aatggctgcg catcgctttg accgatttca
     1201 acgcgccggc gttcgacgtg aagagggcgc cgcagcccga cgtcgcgcag ccgctttcga
     1261 agcgccggcc cggcggtctc ggtatcgccc tggccaaa
//
LOCUS       DASUCJ010000778         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_375349, whole genome shotgun sequence.
ACCESSION   DASUCJ010000778 DASUCJ010000000
VERSION     DASUCJ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375349"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /gene="ftsH"
                     /locus_tag="VFU90_09305"
     CDS             <1..1624
                     /gene="ftsH"
                     /locus_tag="VFU90_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5480017.1"
                     /translation="NSGILQSLFTIAPLALTALFLFLVLRQAQSGGSQALSFGRSRAK
                     MLSENRPKVTFADVAGVDEAKEELAEIVDFLKYPKKYQSLGARIPKGVLLLGPPGTGK
                     TLLARAIAGEAGVPFLSISGSDFVEMFVGVGASRVRDLFDQAKKSAPCIVFIDEIDAV
                     GRQRGAGLGGGHDEREQTLNQLLVEMDGFDQNTGVILIAATNRADVLDPALLRPGRFD
                     RQVVVDRADFKGREKILEVHARNKPLGREVSLRTLAKRTPGFSGADLENLLNEAALLA
                     ARRNKNVIEMIDCDEAIDRVMVGPERKSVVMSAKAKNIAAYHESGHAILGGLLDKSDP
                     IHKVTIIPRGMALGLTWSLPEDDRYQVTREELLAQITMALGGRLSEEIKFGDVTTGAS
                     NDFEKATELARRMVTQYGMSELGPIQYGRGAHQVFLGRDFGDERNYSEEIASKIDAQV
                     RAIIESCYENGRDILNKNWDKVERMVGSLLEYETVDADEVVAILKGEPFDRAAVEDAH
                     APEGESERSVSDEPKRADKPSRLPPKISPEPA"
     gene            1621..>2476
                     /locus_tag="VFU90_09310"
     CDS             1621..>2476
                     /locus_tag="VFU90_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480018.1"
                     /translation="MTPGARLAAAMLACASLLFAVPCGATEAREQQGSLPRGGSYVLE
                     PDPTVSAAAVGLWFRAPGAGYDNATPGVSRLAATAAAIAPLAGGKSLYTLVRSVGGVL
                     NINVYPDIVGIGAVVPSGSARRIVAAMTAAYFAPAIDDATVKVARNDAAVLAVGQRYS
                     FDSTLHDLLFKQIFATGPAHYPPVPDSIAALSRVSPEDVAAFAKRAFRSGNAVLALAG
                     NVDASSLSAVTDGNGNLSMDPPFASTLSGFAGETTASGAVGGVGLAWIGPPIADEKAA
                     TALDFVADY"
BASE COUNT          435 a          800 c          842 g          399 t
ORIGIN      
        1 gaacagcggc atcctgcaga gcctcttcac gatcgccccg ctcgcgctga ccgcgctctt
       61 cctgttcctc gtgctgcgtc aggcgcagag cggcggcagc caagccctct cgttcgggcg
      121 ctcgcgggcg aagatgctct ccgagaaccg cccgaaggtg actttcgcgg acgtcgccgg
      181 cgtcgacgaa gccaaagaag agctggccga gatcgtcgac ttcctgaagt atcccaagaa
      241 gtatcagtcg ctgggcgcgc ggattcccaa gggcgtgctg ctgctgggcc cgccggggac
      301 cggcaagacg ctgctggcgc gcgcgattgc gggcgaagcg ggcgtcccct tcctgtcgat
      361 ttcgggctcg gacttcgtcg agatgttcgt gggcgtgggc gcgtcgcgcg tgcgcgacct
      421 gttcgatcag gccaaaaagt cggcgccgtg catcgtgttc atcgacgaga tcgacgcggt
      481 cggccggcag cgcggcgcgg gacttggagg cggtcacgac gagcgcgagc agacgctcaa
      541 ccagctgctg gtcgagatgg acggtttcga tcagaacacc ggcgtgatct tgatcgcggc
      601 caccaatcgc gccgacgtgc tcgatccggc gctgctgcgg ccgggacgct tcgaccggca
      661 agtcgtggtc gatcgcgccg acttcaaggg acgcgaaaag attctcgaag tgcacgcgcg
      721 caacaaaccg ctgggccgcg aagtttcgct gcgaacgctg gccaagcgca cgcccggatt
      781 ttcgggcgcg gatttggaaa atctcctcaa cgaggccgcg ctgctggcgg cgcgccgcaa
      841 caagaacgtg atcgagatga tcgattgcga cgaggcgatc gaccgcgtga tggtgggccc
      901 ggaacgcaag tcggtggtga tgtcggccaa agccaagaac atcgcggcct atcacgaatc
      961 ggggcacgcg attctcggcg gactgctcga caagtcggat ccgattcata aggtaacgat
     1021 cattccgcgc gggatggcgc tgggactcac ctggtcgctg cccgaggacg atcgctacca
     1081 ggtgacgcgc gaagaattgc tcgcgcagat cacgatggcg ctgggcggcc ggctctccga
     1141 ggagatcaag ttcggcgacg tgacgaccgg cgcgagcaac gatttcgaaa aggccaccga
     1201 gctggctcgc cggatggtca cgcagtacgg catgagcgaa ctcggcccga tccagtacgg
     1261 ccgcggcgcg catcaagtgt tcctcggccg cgatttcggc gacgagcgca actattccga
     1321 agaaattgcg agcaagatcg acgcgcaagt tcgggcgatc atcgaatcgt gttacgaaaa
     1381 cggccgcgat atcctcaata agaactggga caaagtcgaa cgcatggtcg gctcgctgct
     1441 cgaatatgag accgtcgatg ccgacgaggt ggtcgcgatc ctcaagggcg agccgttcga
     1501 tcgcgccgcc gtcgaagacg cccacgcgcc ggagggcgag agcgagcgtt ccgtaagcga
     1561 cgagcccaag cgcgccgaca aaccgtcgcg cctgccgccg aagatttcac cggaacccgc
     1621 atgacgccgg gggccagact agcggccgcg atgctcgctt gcgcatcgtt gctgtttgcg
     1681 gtgccgtgcg gtgccaccga agcacgcgaa cagcagggat cgctgccgcg cggcggctcg
     1741 tacgtgctcg agcccgatcc gaccgtgagc gcggccgcgg tcggactgtg gttccgcgcg
     1801 ccgggcgccg gctacgataa cgccacgccg ggcgtttcgc ggctagccgc taccgccgcc
     1861 gcgatcgcgc cgctggccgg cggaaagtcg ctgtacacgc tggtgcgcag cgtcggcggg
     1921 gtcctgaaca tcaacgtcta tcccgacatc gtcggaatcg gcgcggtcgt gccgtcggga
     1981 tcggcgcggc gcatcgtcgc ggcgatgacg gccgcctatt tcgcgccggc catcgacgac
     2041 gcgaccgtaa aagtcgcgcg caacgacgcg gcggtgctcg ccgtcggtca gcgctactcg
     2101 ttcgactcga cgctgcacga tctgctcttc aagcaaatct tcgcaaccgg gccggcgcac
     2161 tatccgccgg tgcccgattc gatcgccgcg ctttcgcggg tctcgccgga agacgtagcc
     2221 gcgtttgcca aacgcgcctt tcgctcgggc aacgcggtgc tcgcgctggc gggcaacgtc
     2281 gacgcgtcgt cgctttcggc ggtgacagac ggcaacggca acctgtcgat ggatccgccg
     2341 tttgcctcga ccctctcggg attcgccggc gaaacgaccg catcgggtgc ggtgggcggc
     2401 gtcggcttag cgtggatcgg gccgccgatc gccgacgaaa aagcggcgac cgcgctcgac
     2461 ttcgtcgccg actatc
//
LOCUS       DASUCJ010000779         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1475385, whole genome shotgun sequence.
ACCESSION   DASUCJ010000779 DASUCJ010000000
VERSION     DASUCJ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475385"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFU90_09315"
     CDS             <1..458
                     /locus_tag="VFU90_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5480019.1"
                     /translation="PGRDVRTVQLGTARFALRVAHGVLGAAPLLRRVLEDSVARKETM
                     PARLVARYLAPYVGPDGVTHLLTIARALKADDVEELDLALIRAPVAIVWGEEQPWLDS
                     GLPERLQAAIAGSSLVRFHGTGALVPEENPDALAHVVFELLDRQRSAAS"
     gene            562..852
                     /locus_tag="VFU90_09320"
     CDS             562..852
                     /locus_tag="VFU90_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480020.1"
                     /translation="MAKQRNRRRETPAPTPFEQARDELFQHIMRCGVIEAVPEHQEEW
                     FHDTMPYLQDRYPELSDGELSELRTLGTRFCRPPRAKTEGETNEPADAVSAA"
     gene            complement(874..1359)
                     /locus_tag="VFU90_09325"
     CDS             complement(874..1359)
                     /locus_tag="VFU90_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480021.1"
                     /translation="MQARIAVAAFLVWTGVLAPPRVSAQRESGLQAGARFDVMASSIT
                     AVQAGVEMSKLASTNVRVGLTLAGGESSGHGRTGFSARADLVGRFLLDPNFRMRWAPY
                     TGGGLGLRYDTIGDWRGTIIAVIGVEGPNWGGVVPFAEGGFGGGVRLGVGFRATRRVG
                     R"
     gene            complement(1360..1884)
                     /locus_tag="VFU90_09330"
     CDS             complement(1360..1884)
                     /locus_tag="VFU90_09330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5480022.1"
                     /translation="MTSSRVLTVPNLLSLSRLPLAAAFLLVDGRSARVALVGLASLTD
                     FLDGWLARRGQTSQLGALLDPIADKTFVLIAISAFAIEGAVTAGEFVIVISRDLATAM
                     GFIVAYLLPGLDPRAFKARWPGKVVTVLQLAALFALLLRPSVFPALIPLIAVASAAAI
                     ADYTLVLHRQRVRS"
     gene            2024..2488
                     /locus_tag="VFU90_09335"
     CDS             2024..2488
                     /locus_tag="VFU90_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5480023.1"
                     /translation="MPATIDVLRNLPIFSGLSTDELERVSELCEEKTYASGDYIFREG
                     EPGNRLFIVIEGEVRISRQIPGAGEEALAVLKPGAMFGEMAVFDRSERSTDAISHGGT
                     RVITISRAEFEMLLDFHRDIAYKVLWAVVRVLSGRLRATNDNLRSVLAMSMF"
BASE COUNT          487 a          790 c          813 g          405 t
ORIGIN      
        1 gtccggggcg tgacgtgcgc accgtgcagc tcggcacggc gcgcttcgcg ttgcgcgtcg
       61 cgcacggcgt gctcggggcg gcaccgttgc tgcgtcgtgt cctggaagac agtgtggctc
      121 gaaaggaaac gatgcccgca cgtctcgtgg cgcggtacct cgcaccgtat gtcggccccg
      181 acggtgtcac gcacctgctc acgatcgctc gagcgctcaa ggcggacgac gtcgaagagc
      241 tcgatctggc gttgattcgt gcaccggtcg cgatcgtttg gggcgaggaa cagccgtggc
      301 tggacagcgg tctgcccgag cggctgcagg ctgcaatcgc cggaagcagt ctggtgcgtt
      361 ttcacggcac cggcgctctc gtgccggaag agaacccgga cgcgctggca cacgtcgttt
      421 tcgagctgct cgatcgtcaa cggtcggcgg cgtcgtaacg taacgtatgg ggagcccaat
      481 gaattcggca tccgacgcgc gagagcttca atcctcgcgc gaagcacaat atatttcgtt
      541 cacttcagcc aacaggattg aatggcaaaa cagcgcaatc gtcgccgtga gacccctgca
      601 ccgacaccat tcgaacaggc acgcgacgaa ttatttcaac acatcatgcg gtgtggcgtg
      661 atagaggcgg tcccggagca tcaggaagag tggttccacg ataccatgcc gtacctgcag
      721 gaccgctacc cagaattgtc ggacggcgag ctttctgaac tgcgcacgct tggcacgagg
      781 ttctgccggc ctccaagagc gaaaaccgaa ggcgagacca acgaacccgc tgacgcggtt
      841 tccgctgcct gatctttggg ctcggcgcga cggctaccgg ccgacacgac gtgtcgctcg
      901 gaatccgacg ccgagccgaa cacccccgcc aaatcctcct tcggcgaagg gcaccacgcc
      961 gccccaattc ggcccttcga cgccgatgac ggcgatgatg gtcccgcgcc aatcgccgat
     1021 cgtgtcgtag cgcaggccaa gcccgccgcc tgtgtatggc gcccaacgca ttcgaaaatt
     1081 cggatccagc agaaagcgtc cgaccaaatc ggcccgcgcg ctgaaacccg tgcgtccatg
     1141 ccccgatgat tcgccaccgg cgagcgtcag gccgactcgc acgttggtcg acgcaagctt
     1201 gctcatttca acgcccgcct gcaccgccgt gatgctcgac gccatgacgt cgaagcgtgc
     1261 gcccgcttga agtccagatt cacgctgcgc ggatacgcgc ggcggcgcca acactcccgt
     1321 ccagaccaga aacgccgcca ccgcgatgcg cgcctgcatt cacgaccgaa cgcgctggcg
     1381 gtgcaacacg agggtgtagt ctgcgatcgc cgccgcggac gcaacggcga tcagcggtat
     1441 caacgcgggg aacaccgagg gccgcagcaa cagtgcaaac agcgcggcga gctgcagcac
     1501 ggtcacgacc ttcccgggcc atcgtgcctt gaaggcacgc ggatcgaggc ccggcaggag
     1561 atacgcgacg atgaatccca tcgccgtcgc cagatcacgc gaaatgacga tgacgaactc
     1621 ccctgccgtc accgctcctt cgatcgcgaa tgccgagatc gcgatcagca cgaacgtctt
     1681 gtcggcgatg ggatcgagca acgcgccgag ttgcgacgtt tgtccgcggc gcgcgagcca
     1741 tccatcgagg aaatcggtga gggacgcgag cccaacgagc gccacgcgcg ccgagcgacc
     1801 gtcgacgagc agaaacgcgg ccgccagcgg gagacgtgag agcgacagga gattcggaac
     1861 cgtcaggacg cgagacgaag tcacggtgag tcaacttaga acaggtggga catcgcttcc
     1921 agccggtgag cgctggcggt tctcacgacg gaacgttcgg tccgggggtt tcgcagcgcg
     1981 gcttcgcgtg taggttggag cggctcactg cacgagggag gcaatgccgg ccacgatcga
     2041 cgtgttgcgc aatttgccga tcttcagcgg actatcgacc gacgagctgg agcgcgtgtc
     2101 ggagttgtgc gaagaaaaga cctatgcatc gggcgactac atctttcgcg agggcgagcc
     2161 gggaaaccgt ctcttcatcg tgatcgaggg agaagtgcgg atcagccgcc agattccggg
     2221 cgccggcgag gaggcgttag ccgtgctcaa gcccggcgcc atgttcggcg agatggcggt
     2281 gttcgaccgg agcgagcgct caaccgacgc catctcgcac ggcggcacgc gggtgatcac
     2341 gatcagccgg gccgaattcg agatgttatt ggattttcat cgcgacattg cgtacaaggt
     2401 gctgtgggcg gtggtgcgcg tgttgagcgg ccggttgcgc gccaccaacg acaacctgcg
     2461 ctcggtgctc gcgatgtcga tgttctgagc gcgcg
//
LOCUS       DASUCJ010000780         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1337954, whole genome shotgun sequence.
ACCESSION   DASUCJ010000780 DASUCJ010000000
VERSION     DASUCJ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337954"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFU90_09340"
     CDS             <1..1363
                     /locus_tag="VFU90_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480024.1"
                     /translation="RKGFHVAIVRGFSCFLALSAGLTGCSGLGRSLPPASRAAAGTTV
                     NFYSAQASLPSPIRHVVIIFQENRTADYMFQDVPGADVAKYAIDSRGKRVALLPVSLA
                     APYGLAHSRGAFLRDYDGGKMDGFDSRLPPGERLRPYGYGVRSEIRPYRDMAVQYVAA
                     DHMFQSNRGPSYPAHLYIVSGTATEPKIDDYRVSANPFDTRTKRPEGGGCDARPGTVV
                     ATAGLQRGGRGPALFPCLDRPVLPDLLDERHVSWRYYQHGRGPGLWHGLNSIRHVRFG
                     PDFKNVVSPSETILTDIARGRLAGMSWVMPDPEHSDHAGSNSAAGPSWVAAVVNAIGK
                     SQYWNNTAIFVTWDDWGGWYDHVPPPVDNACELGFRVPLIVISPYAKRGYVSKVQHEF
                     GSLLAFTEETFGIPKGALGATDKRADDLSDAFDFTQKPRAFVPIKAPPFSPGPPNDQS
                     EDP"
     gene            1488..2858
                     /locus_tag="VFU90_09345"
     CDS             1488..2858
                     /locus_tag="VFU90_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480025.1"
                     /translation="MEVRNGASIGTRTLTAALTALAVTAALAGCNGHAQSFVPTAPAG
                     GAAAFYDPAAQLPTPIRHVVIIFQENRTADYMFQDVPGADVTKSAIDSRGEPVRLHEV
                     SLATTYDLGHYHRDFVADYNGGRMNGFDSRLPDGKRALPYGYGIPSEIRPYHDMAVQY
                     VAADHMFQTNQGPSYPAHLYIVSGTATEPQIRNYRVEDNPFQAVTRKWRAGGCASAPD
                     VLVSTIDVHTGSAGPDVYPCFDRPVLSDFLDRQHVSWRYYQQQSQNGLWHALNSIRHV
                     RFGPGFSNVISPPQTILSDVAAGRLAGVSWVMPDNVHSDHASNRSAAGPSWVAAVVNA
                     IGRSKYWNSTAIFITWDDWGGWYDHVKPPIDNAYELGFRVPLIVVSPYAKRGYVSKNR
                     HEFGSILAFAEVTFGIPKGALNSTDVRADDLRDTFNFAQSPRTFVPIKAPPFQPGPAS
                     DRSEDP"
     gene            complement(2973..>3388)
                     /locus_tag="VFU90_09350"
     CDS             complement(2973..>3388)
                     /locus_tag="VFU90_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480026.1"
                     /translation="FIPAAGSGAAAAFYNPAGQLPTPIRHVVIIFQENRTPDYMFQDV
                     PGANVVKSAIDSYGERVYLHKQSLAVAYDLGHRRRDFVGDYNGGKMNGCARPLHPVSA
                     MPNARSATSEGVVRTPLRAKRPTEAVILLSPPIRR"
BASE COUNT          621 a         1089 c         1073 g          605 t
ORIGIN      
        1 ccgaaagggg tttcacgtgg cgatcgttcg cggattttct tgctttttgg cgctgagcgc
       61 cgggctgacg ggctgctccg gactcgggcg atcgctgccg ccggcaagcc gcgccgccgc
      121 tggtacgacc gtaaacttct actcggcgca agcgtcgttg ccgtcgccga ttcgtcacgt
      181 cgtcatcatc tttcaggaga accgcacggc cgactacatg ttccaggacg ttccgggagc
      241 cgacgtcgcc aaatacgcaa tcgactcgcg ggggaagcgc gtcgcgctgc tgccggtatc
      301 gctggccgct ccttacgggc tcgcgcattc gcgcggcgcg tttctccgcg attacgacgg
      361 cggaaaaatg gacggcttcg attcacggct tccacccggc gaacggctgc gtccgtacgg
      421 ctacggggtt cgttccgaaa ttcggccgta tcgcgacatg gcggtccagt acgtcgccgc
      481 cgatcacatg ttccagagca atcgcgggcc gagttatccg gcgcacctct atatcgtgag
      541 cgggacggcc acggagccga aaatcgacga ctatcgcgtt tcggccaatc cgttcgacac
      601 gcgcaccaag cggccggaag gcggcggctg cgacgcacgg ccgggaacgg tcgtcgcgac
      661 cgccggtttg caacgcgggg gccgcgggcc ggcgttgttt ccgtgtctcg atcggccggt
      721 gctgcccgac ttactggacg agcggcacgt gtcgtggcgg tattatcagc acgggcgcgg
      781 acccggttta tggcacggcc tcaattcgat ccggcacgtg cgttttggcc ccgatttcaa
      841 gaacgtcgtt tcgccgtcgg agacgatcct cacggatatc gcgcgcgggc ggctggcggg
      901 aatgtcgtgg gtgatgcccg atccggagca ctcggatcac gccggcagca acagcgcggc
      961 cgggccgtcg tgggttgcgg cggtggtgaa cgcgatcgga aagagccaat actggaacaa
     1021 caccgcaatt ttcgtgacgt gggacgattg gggcggctgg tacgaccacg tgccgccgcc
     1081 cgtcgacaac gcctgcgaac tcgggttccg cgtgccgctg atcgtgatct ctccgtacgc
     1141 aaagcgcggc tacgtctcga aagtgcagca cgaattcggc agcctgctgg cctttacgga
     1201 agaaacgttc ggaataccga aaggcgcgct gggcgccacc gacaaacgcg ccgacgatct
     1261 gagcgacgcc ttcgacttta cgcaaaagcc gcgggcgttc gtgcccatca aggccccgcc
     1321 gttttccccg ggtccgccga acgaccagtc cgaagacccg tagctccccg cgccggtcgc
     1381 gcgcgcgccg agcgcagtag atgagaagac ggtcgtcgag atagttcacg acatcttccc
     1441 atcgcggccg tatgctcttc cggtaaagac tgagagggga gcaaacgatg gaggttcgca
     1501 acggggcttc catagggacg cgtacgctga cggcagccct aaccgccctc gcggtgacgg
     1561 ccgcgctggc cggctgcaac ggacacgcgc aatcgttcgt gcccaccgcc cccgccggcg
     1621 gcgccgccgc gttttacgat cccgcggcgc agctgccgac gccgattcga cacgtcgtta
     1681 tcatcttcca ggagaaccgg acggccgatt atatgttcca ggacgttccg ggagccgacg
     1741 taacgaagag tgcgatcgat tcgcgcggcg agcccgtgcg gcttcacgag gtttcgctag
     1801 cgaccaccta cgacctaggt cactatcacc gggacttcgt tgccgattac aacggtggcc
     1861 ggatgaacgg ctttgattcg cgtctacccg acggcaagcg ggcgctccca tacggttatg
     1921 gtattccgtc ggagatccgg ccgtaccacg acatggccgt gcagtacgtc gccgcggatc
     1981 acatgttcca aaccaatcag ggtccaagct atccggcgca tctctacatc gtgagcggaa
     2041 cggcgaccga gccacagatc cgaaactatc gcgtcgaaga caatccgttt caggcggtca
     2101 cccgcaagtg gcgtgcgggc ggttgcgcga gcgcgcccga cgtactcgtc tccacgatcg
     2161 acgtccacac gggaagtgcc ggtccagacg tatacccatg cttcgaccgt cccgtgctct
     2221 ccgatttctt ggaccggcag cacgtctcgt ggcgttatta tcaacaacag tcccagaacg
     2281 gcctctggca cgcattgaac tcgattcggc acgttcgctt tgggcccggc ttctcgaacg
     2341 tcatttcgcc gccacagacg attttgtcgg acgtcgcagc gggacggctc gccggcgtct
     2401 cgtgggtgat gcccgacaac gtgcactcgg accacgcgag caatcgaagt gccgcggggc
     2461 catcgtgggt ggcggcggtc gtcaacgcga tcggtcgaag caagtactgg aacagcacgg
     2521 caatcttcat aacgtgggac gactggggcg gatggtacga ccacgtcaag ccgccgatcg
     2581 ataacgcgta cgagctcggc ttccgcgtgc ccctgatcgt cgtctcgccg tacgccaagc
     2641 gcggatacgt gtcgaagaac cggcacgagt tcggcagcat tctcgccttc gcggaggtga
     2701 cgtttgggat tcccaaggga gctttaaact cgaccgacgt gcgcgccgac gacctccggg
     2761 acacattcaa cttcgcgcag agcccgcgca cgttcgtgcc gatcaaagcc ccgccgttcc
     2821 agcccggccc ggcgagcgat cgatccgagg acccgtgacc aacgcgcggc cgtggcgcaa
     2881 taccggcgcc ccggaaatga gaagaagatc gaggccgcag ttcatgagtt cttcgcatcg
     2941 cggtcgtatg ctcgacgcga aaacgggcac gctcatcgcc gaattggagg agacagcaag
     3001 atgaccgctt ctgtaggacg cttcgcacgc aagggcgtcc gaacgacccc ctcactcgtg
     3061 gcgctcctcg cgttcggcat cgcgctgacc ggatgcaacg ggcgcgcgca gccgttcatc
     3121 tttccgccgt tatagtcgcc gacgaaatcg cggcgacggt gccccaagtc gtacgcgacc
     3181 gctaacgact gcttatgtaa atacactcgc tcgccgtagg aatcgatcgc gctcttgacg
     3241 acgttcgccc ccggaacgtc ttgaaacatg tagtcgggcg tgcggttttc ctgaaagata
     3301 atgacgacgt ggcggatcgg ggtcggcagt tgccccgcgg ggttgtagaa tgcagcggcg
     3361 gcgccggaac cggctgccgg aatgaacg
//
LOCUS       DASUCJ010000781         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1016995, whole genome shotgun sequence.
ACCESSION   DASUCJ010000781 DASUCJ010000000
VERSION     DASUCJ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFU90_09355"
     CDS             <1..350
                     /locus_tag="VFU90_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480027.1"
                     /translation="PSCRGAIDDVSRIIGRVVIEEGARVERSTVRGPAIIGRGTAIVD
                     SYVGPFTSIYHDCRIERTELEHSIVLESTTIVGVSRIEDSLIGKEVEVSPSTAMPRAH
                     RLMLGDHSKVSLA"
     gene            419..>1114
                     /locus_tag="VFU90_09360"
     CDS             419..>1114
                     /locus_tag="VFU90_09360"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEU5480028.1"
                     /translation="MVAAGYTLFEVQEQRHFRTAGYDLGIFAQAIHGYAHFHAPVSLM
                     KGVHNGFGPNFSILGDHFSPILAVLGPVYAVFPHPTTLLVAQGLLFAASVPFVWLFAQ
                     RKLGRVPAYLLALAYALSWGLQTAMDADFHEVAFAVPLLAIAIERLDAGRLRAATVAA
                     LLLLLVKEDYGLVVAMFALVVGVRTKRWRFAGVLALIGVVAPVVADKVFIPLAGGKSG
                     YYWDYYAALGANPL"
BASE COUNT          153 a          361 c          383 g          217 t
ORIGIN      
        1 agccgtcgtg ccggggcgcg atcgacgacg tcagccggat catcggccgg gtcgtcatcg
       61 aagagggcgc ccgtgtcgag cggtcaaccg tgcgcgggcc ggcgatcatc gggcgcggca
      121 ccgcgatcgt cgacagttac gtcgggccgt tcacgtcgat ctaccacgac tgtcggatcg
      181 agcgaaccga gctggagcac tcgatcgtgt tggagtcgac gaccatcgtc ggcgtcagcc
      241 gcatcgagga ttcccttatc ggtaaggaag ttgaggtgtc cccatcgacc gcgatgccac
      301 gtgcgcaccg gttgatgctc ggcgaccaca gcaaggtgtc gctcgcctga gcgcgctcgg
      361 cgagcgcacc cccggccggc ggcggctcga cgtcgccgca gtcagcgcgc tgacgttggt
      421 ggtcgcggcc ggctacacgc tgttcgaagt gcaggaacag cgccacttcc gcaccgcggg
      481 ctacgacctc ggcatcttcg cgcaggcgat tcacggctac gcgcactttc acgcgccggt
      541 gtcgttgatg aagggcgtgc acaacggctt cggcccgaac ttctcaatcc ttggcgacca
      601 tttctcgccg attctggccg tgctggggcc ggtctacgcg gtgttcccgc atcccacgac
      661 tttgctggtc gcgcaggggt tgctgttcgc ggcgtccgtg cccttcgtct ggctgttcgc
      721 gcaacgcaag ctcggccggg taccggccta cctgctggcg ttggcgtatg cgttgtcgtg
      781 gggattgcag accgcgatgg acgccgactt tcacgaggtc gcgttcgccg tcccgttgct
      841 cgcgatcgcg atcgagcggc tcgacgcggg gcggttgcgc gccgccacag tggccgccct
      901 cttgttgctg ctcgtcaagg aggactacgg cctggtcgtc gcgatgttcg cattggtggt
      961 cggggtgcgc accaagcggt ggcgattcgc cggagtgctg gcgctgatcg gggtcgtcgc
     1021 gcctgtcgtc gctgacaagg tgttcatccc gctcgccggc ggaaaatccg gctattactg
     1081 ggattactac gcagcgcttg gcgccaatcc gctg
//
LOCUS       DASUCJ010000782         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_833352, whole genome shotgun sequence.
ACCESSION   DASUCJ010000782 DASUCJ010000000
VERSION     DASUCJ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833352"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2635)
                     /locus_tag="VFU90_09365"
     CDS             complement(<1..2635)
                     /locus_tag="VFU90_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5480029.1"
                     /translation="MIEVFLKRPLFAGVLSFVVLLAGLICIPTLPISQYPQISPPTVT
                     VTANYPGADAEAVVRSVTTPLEININGADGLQYMQSYSSSNGVATITCTFALGTDPTT
                     DQTNVQHGVDLTLSQLPTAVQQQGVTVQKSSGNITIAVAMLSTSSSVSDVAVSNYADI
                     NVIEDLKRIPGVGQVEVLGDRTYGMRIWVDPHKLQANNVSLDNVINAIQQQNANVAPG
                     AIGQAPTTGSQPYQIPIKVNGRLQSPQEFKQIVVAVQPNGGYLRLGDVATVELGAQNY
                     VTSARYNQQTAVVLAIEGEPTANSLQISKSVRATMERLKPYFPTGMDYSIAFDTSDFV
                     RASIKEVIVTLLIAILLVVLVIFIFLQNWHSTLIPSITIPVSLIGTFAAMKVLGFTIN
                     TLTLFGLTLATGLVVDDAIVVLENIVRHIVDDKVGGFEATKRAMKEISGAVVATSLVL
                     MSVFIPVAFLPGTTGLLYQQFALTMAASIGISLFTALTLAPVLTYELVHSEEPSKNPF
                     MRWFRDRLDGLRTWYSGVVPRLVARRKMMVAIFVGGIVLLGILFKVTPQGFLPTEDQS
                     LLYVLVTLPTSASMDQTTAVVKRLEAMMLKMPQVEAVTSGIGFGFANTSSNQATIFVQ
                     LHPLADRRGLENSSLNLQYQLYESFQKIPGMTALPANPPAIPGLGSTGGFAIEIQDIN
                     GLGLPTLNRVGNQILAQAQKDNRLSQVRIPTLLTGPYVVAKFNRAKALALGVSPTSFF
                     DTLNATTGSDFVNFFDYGSRSYDVLVQAGAKHRTTPQDLSRIYVPNSSGDMMPVSQFL
                     DISRQTANVAIMRFNEFNAYEIDGSPGTSASSGQALDAIAQIATKNMPKGMKYDWSGI
                     ARQQVQSGPQTILVFAMGLLFV"
     gene            complement(2726..4402)
                     /locus_tag="VFU90_09370"
     CDS             complement(2726..4402)
                     /locus_tag="VFU90_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5480030.1"
                     /translation="MFAREKYLPNNLRPHNYSTNDLFGASAIFKVALLAIGLLAGGCT
                     RGGEPAAPAGTLRFDVAADPQTLNPLFLHPDAASVEHQLARLIFEPFIDLDSRGRPVP
                     ALLSEIPTRADGGLSADGRTIVYRLRPGVVWSDGRPVTAEDVLFTLRAILDPRNPVRS
                     HEGYDLIDAVAARDARTVVVHLKRAWAPAVMTYFSYGFAPQFVLPAHVLRAQTPLAQA
                     AFNAAPTVGDGPYRFVEWRRGEGLRYAANPRYWRGKPAVEALDVRVVPDPSTNLLLLQ
                     SGQVDWNLLAPAQLAVLSGKPGIDYVRVPTAVVAGIAFNTRRPPLDDVAIRRAIAMSI
                     DRDAISRKIALGIYPVTNVIQPQFSWAYDPAVREPAYDPAGADALFAAAGWRRGSDGT
                     LRKAGRRLALTYVQFPESTTGVRVATAIQAALRERGIDVAIKSISNAQLFLPKTGALA
                     SGAFDLAYVPWTMGADPDDSAVLACGGASNYMGWCDPSVDALERDALSATGQPSRKRL
                     YARIGRIVARQVPILYLFNADYVYAFRSRLRGFSPNPFLPTWNAGDWRLR"
     gene            4401..4871
                     /locus_tag="VFU90_09375"
     CDS             4401..4871
                     /locus_tag="VFU90_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5480031.1"
                     /translation="MITTTEELDELDLQLLDALQRNARSTFAELGSIIGLKAPAVHDR
                     VKRLEQRGYIQRYSAQLDAKRLGLQLTAFVSLYTAPDCDYDEFTRALGEMPEVCEVHS
                     VAGEETFVCKVVTGSTKHLDDLLARLKGMPGMARTRTTIVLTTPYDRGGITVRS"
     gene            4868..5950
                     /locus_tag="VFU90_09380"
     CDS             4868..5950
                     /locus_tag="VFU90_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HEU5480032.1"
                     /translation="MNAVSQRRLHPLGTHRVLEPAGAMPQTAWRLDNTPVAFANEILC
                     RVETLNIDSASFKQISQACDGDAERIAAHVTNLVEQRGKQHNPVTGSGGMFIGAVAEI
                     GEALRAKIDLRPGDRIASLVSLTLTPLHLEAVTRVDVATGRVWIRGHAILFESGLWAR
                     LPEDIDENVALAVLDVAGAPAQVARLCAPGQTVVVIGADGKSGMLSCAQAKARTGKNG
                     RVIGVVPDPSTPSSQLLLRAGLVDALIAADARDALDVSEKVAAAAPQLADVVINCVNV
                     PDTELGSILCAKQNATVYFFSMATSFTAAALGAEGVGRDVTMIVGNGYTQGHARIALQ
                     TLRDHPDVLAHFNEKYANSQPEEFSR"
     gene            5947..>7246
                     /locus_tag="VFU90_09385"
     CDS             5947..>7246
                     /locus_tag="VFU90_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU5480033.1"
                     /translation="MNDTIHHLKPPAPPDQFEYKNLKQGEFWRHIPAYKDVDEATFLD
                     HLWQQKHAVKTSAELLETIKDVCTPEFFADAAAGFSRAPMAVRVSPYAISLIDWSDPI
                     ADPIRRQFVPLASALLPDHPRLTLDSLHEQADSPVPGLVHRYVDKALFLPLNTCPVYC
                     RFCTRSYAIGPDTENVDKVALAKTPKQWEDAFKYVAERPELEDIVISGGDTYQLPPKN
                     VELIGNALLDIPHVRRMRFATKGPAIMPMKILTHTEWLDAITRIVERGRTMGKEVVLH
                     THFNAPEEITWITEKAMRMLFERGIFTRNQSVLIRGVNDTRERMQLLVKRLGHLNVHP
                     YYVYMHDMVKGVEELRTTIHTATDTEKFVRGSTAGFNTPTFVCDAPGGGGKRDVHSYE
                     YYDRENGIAVYAAPSVKPGKAFLYFDPIDRLSPDAQARWAV"
BASE COUNT         1344 a         2350 c         2345 g         1207 t
ORIGIN      
        1 agacgaacag cagccccatc gcaaacacca gaatcgtctg cggtccgctc tgcacttgct
       61 ggcgcgcgat cccgctccag tcgtacttca ttcccttggg catgttcttg gtggcgatct
      121 gcgcgatcgc gtcgagcgcc tgccccgagc tggcgctcgt cccgggcgaa ccgtcgattt
      181 cgtacgcgtt gaactcgttg aaacgcatga tcgccacgtt ggcggtttgg cgcgaaatgt
      241 ccaagaactg cgagaccggc atcatgtcgc cgctcgaatt cggtacgtag atgcgcgaca
      301 ggtcctgcgg cgtcgtccgg tgtttggcgc cggcttgaac gagcacgtcg tacgagcggc
      361 ttccgtaatc gaagaagttg acgaagtccg atccggtcgt cgcgttgagg gtatcgaaga
      421 aactcgtggg cgacacgccc agcgcgagcg ccttggcgcg gttgaactta gcgacgacgt
      481 acgggcccgt cagcagcgtg ggaatgcgaa cctgcgagag ccggttgtct ttctgcgctt
      541 gcgcgagaat ctggttgccg acgcgattga gggttgggag cccgaggccg ttgatgtcct
      601 ggatttcgat cgcaaaaccg ccggtcgagc ccagccccgg aatcgccggg ggattggccg
      661 ggagcgcggt catccccgga atcttttgaa agctttcgta gagctgatac tgcaggttca
      721 gcgagctgtt ctccaggccg cggcgatcgg cgagcgggtg cagctgaacg aagatcgtcg
      781 cctggttgga gctggtgttg gcgaatccga aaccgatccc cgacgtgacc gcctcgactt
      841 gcggcatctt cagcatcatc gcttcgagcc gcttcacgac cgcggtcgtc tgatccatcg
      901 acgcgctggt cggcagcgtc acgagcacgt agagcagact ctgatcttcc gtcggcaaga
      961 agccctgcgg cgtgactttg aagaggatcc cgagcagcac gattccgccc acgaagatcg
     1021 ccaccatcat cttgcggcgg gcgaccagcc tcggaaccac gcccgaatac caggttctca
     1081 acccgtcgag ccggtcgcga aaccagcgca tgaagggatt cttgctcggc tcctcgctgt
     1141 gcacgagttc gtaggtcaat accggcgcga gcgtcagcgc ggtaaacagc gagatgccga
     1201 tcgaggccgc catcgtcagc gcgaactgct ggtagagcag gccggtagtc cccggcagga
     1261 aggcgaccgg gatgaagacc gacatgagca ccagcgaggt cgcgacgacg gcgccgctaa
     1321 tttctttcat cgcgcgcttg gtcgcctcga aaccgccgac cttgtcgtcg acgatgtggc
     1381 gaacgatgtt ttccagcacg acgatcgcgt cgtcgacgac gagcccggtc gcgagcgtca
     1441 agccgaagag cgtcagcgtg ttgatcgtaa agcccagcac cttcatcgcg gcgaaggttc
     1501 cgatcagcga taccgggatc gtgatcgagg ggatcagcgt gctgtgccag ttctggagga
     1561 agatgaagat caccaagacg accagcagaa tcgcgatcag cagcgtgacg atcacctcct
     1621 tgatcgacgc gcgcacgaaa tcggaagtgt cgaaagcgat cgagtagtcc atcccggtcg
     1681 gaaagtacgg cttgagccgc tccatcgtgg cccggacgct cttggaaatc tgcagcgaat
     1741 tggcggttgg ttcgccttcg atcgcgagca ccacggccgt ctgctggttg tagcgtgccg
     1801 acgtcacgta gttctgcgcg cccagctcga cggtggcgac gtcgccgagc cgcaagtagc
     1861 cgccgttggg ttggaccgcg accacgatct gcttgaactc ttggggcgac tgcagccgcc
     1921 cgttgacctt gatgggaatc tgatacggct gcgatccggt ggtcggcgcc tgtccgatcg
     1981 cacccggcgc gacgttggcg ttttgctgct ggatcgcgtt gatgacgttg tcgagcgaga
     2041 cgttattggc ttgcagcttg tgcgggtcga cccagatacg catgccgtac gtgcggtcgc
     2101 ccagcacttc tacttgtccc accccgggaa tgcgcttgag atcctcgatg acgttgatgt
     2161 cggcgtaatt gctcacggcg acgtcgctca ccgacgaact cgtcgacagc atggcgacgg
     2221 cgatcgtaat gttgcccgag ctcttttgga cggtgacgcc ctgctgctgc acggcggtgg
     2281 gaagctggga gagggtgagg tcgacgccgt gctggacgtt ggtctgatcg gtcgtcgggt
     2341 cggttcccag cgcgaacgtg caggtaatgg tcgcgacgcc gttggagctc gaatacgact
     2401 gcatgtactg caggccgtcg gcaccgttaa tgttgatctc gaggggcgtc gtcacggagc
     2461 gcacgaccgc ctcggcgtcg gcgccgggat agttggccgt gacggtaacc gtcggcggcg
     2521 aaatctgcgg gtattgcgaa atcggcaggg tcggaatgca gatgaggcct gccagcaata
     2581 ccacgaaact caaaacgccg gcaaaaagcg gacgtttgag aaagacttcg atcaacgact
     2641 tactccgatc agtcgcattc gctccgccgt gaagctcgcc taccaagcct aagtatcggc
     2701 acgcccggcc ttctcccttc gcgtgctacc gcagccgcca gtcccccgcg ttccacgtcg
     2761 gaaggaacgg attcggggaa aagccgcgaa gccgcgagcg aaacgcgtag acgtaatcgg
     2821 cgttgaaaag gtacaagatc gggacctgcc gcgcgacgat tcggcctata cgcgcgtaaa
     2881 gccgctttcg cgacggttgc ccggtcgcgc tcaaggcgtc ccgctccagc gcgtcgacgg
     2941 acgggtcgca ccagcccata tagttcgagg ctccgccgca ggcgagcact gccgaatcgt
     3001 cgggatccgc acccatcgtc cacgggacgt aggcgaggtc gaacgctccg gaggcgagcg
     3061 cacccgtttt cgggagaaac agctgcgcgt tcgagatcga ctttatcgcg acgtcgatgc
     3121 cgcgctcgcg cagcgccgcc tgaatcgccg tcgcgacgcg cacgccggtc gtcgattccg
     3181 gaaactgcac gtaggtgagc gccaaccggc gcccggcttt ccgcaacgta ccgtcgctgc
     3241 cgcgacgcca gcccgccgcc gcgaacagcg cgtcggcgcc cgccggatcg tacgccggct
     3301 cgcgcaccgc cggatcgtac gcccacgaga actgcggctg aatcacgttc gttaccggat
     3361 agatcccgag cgcgatcttg cgcgagatcg catcgcggtc gatcgacatc gcgatcgccc
     3421 ggcggatggc gacgtcgtcg agcggagggc gccgcgtgtt gaacgcgatt cccgcgacga
     3481 ccgccgtggg aacgcggacg taatcgatgc cgggcttgcc gctcaaaacg gcgagctgcg
     3541 ccggggcgag caaattccag tcgacctgtc cggattgcaa cagcagcagg ttggtggagg
     3601 gatcggggac gacgcgcacg tcgagcgcct cgaccgccgg tttaccgcgc caatagcgcg
     3661 ggttggcggc gtaccgcagc ccttctccgc ggcgccattc cacgaaccgg tacggaccgt
     3721 cgcccaccgt cggggccgcg ttgaacgccg cctgcgcgag cggcgtttgc gcccgcagca
     3781 cgtgcgccgg caatacgaac tgcggcgcga agccgtagga gaagtacgtc atgaccgccg
     3841 gcgcccacgc gcgcttcaag tgcaccacga cggtgcgggc gtcgcgcgcc gcgaccgcgt
     3901 cgatcaggtc gtagccttcg tgcgaacgca cgggattgcg cggatccagg atcgcgcgca
     3961 gcgtaaacag cacgtcctcg gcggtgaccg gccggccgtc gctccagacg acgcccggcc
     4021 gcagccggta gacgatcgtc cgcccgtcgg ccgagagccc cccgtcggcg cgggtcggga
     4081 tttcggaaag gagcgccgga accgggcgcc cgcggctatc gaggtcgatg aacggctcga
     4141 agatcaggcg ggcgagctgg tgctcgaccg aggcggcgtc ggggtgaagg aagagcggat
     4201 tgagcgtctg cgggtcggcg gccacgtcga accggagggt tcccgccggc gcggccggct
     4261 cgcccccgcg ggtgcagccc cccgccagca ggccgatggc cagcagggca accttaaaaa
     4321 tggcacttgc gccgaataaa tcgttggtgc tatagttgtg cggccgaaga ttgttcggca
     4381 aatacttctc ccgagcgaac atgataacga ccaccgagga actcgacgaa ctcgaccttc
     4441 agctcctgga cgcgttgcag cgcaacgcgc gttctacctt tgcggaactc ggttcgatca
     4501 tcgggctcaa agcgccggcg gtgcacgacc gggtgaagcg gctcgaacag cgcggctaca
     4561 tccagcggta ttcggcgcag ctcgacgcca aacggctggg actgcagctg acggcgttcg
     4621 tcagtctcta caccgcgccc gactgcgact acgacgagtt cacgcgtgcg ctcggcgaga
     4681 tgccggaggt ctgcgaagtc cactcggtcg ccggcgagga gacgttcgtt tgcaaagtgg
     4741 tgaccggctc caccaagcac ctcgacgacc tgctcgcccg cctgaaagga atgccgggga
     4801 tggcacgcac gcgtacgacc atcgtcctga ccacgcccta cgatcgcggc ggaatcacgg
     4861 tgcgctcgtg aacgccgtat cgcagcgccg gctccacccg ctcggcacgc atcgcgtgct
     4921 cgaacccgcg ggcgcgatgc cgcagaccgc gtggcgtctc gacaacacgc cggtagcgtt
     4981 cgccaacgag attctttgcc gcgtcgaaac gctcaacatc gattccgcgt cgttcaagca
     5041 gatttcgcaa gcgtgcgacg gcgatgcgga acgcatcgcc gcccacgtca ccaacctggt
     5101 ggagcagcgc ggcaagcagc ataacccggt caccggcagc ggcgggatgt tcatcggcgc
     5161 ggtggccgag atcggcgagg cgctgcgcgc gaagatcgat ctgcggccgg gcgaccggat
     5221 cgcgtcgctg gtgtcgttga cgctgacgcc gctgcacctc gaagcggtga cgcgcgtcga
     5281 cgtcgcgacc ggacgcgtct ggatccgggg acacgcgatt ttgttcgaga gcggcctctg
     5341 ggcgcggctg ccggaggaca tcgacgagaa cgtcgcactg gccgtgctcg acgtcgccgg
     5401 cgctcccgct caggtagcgc ggctgtgcgc gcccggccaa accgtcgtcg tgatcggcgc
     5461 ggacggcaag agcggcatgc tgtcgtgcgc gcaagccaag gcccgcaccg gcaaaaacgg
     5521 ccgggtgatc ggcgtcgtgc cggatccttc caccccgtcg tcgcagctgc tgctgcgcgc
     5581 cggtctggtc gacgcgctga tcgcggccga cgcgcgcgac gcgctcgacg tgagtgaaaa
     5641 ggtcgcggcg gccgcgccgc agctggccga cgtcgtgatc aactgcgtca acgtacccga
     5701 taccgagctc ggctcgatac tctgcgccaa gcagaacgcg accgtgtatt tcttttcgat
     5761 ggcaacgtcg ttcaccgccg cggcgctggg cgccgaaggc gtcggacgcg acgtcacgat
     5821 gatcgtcgga aacggctaca cccagggtca cgcccggatc gccctgcaga cgctgcgcga
     5881 tcaccccgac gtgctcgctc atttcaacga aaagtacgcc aacagccaac ccgaggaatt
     5941 cagccgatga acgatacgat ccatcatctc aagccgccgg cgcccccgga ccagttcgaa
     6001 tacaagaacc tcaagcaggg cgagttctgg cgccacattc cggcgtacaa agacgtcgac
     6061 gaagcgacct tcctcgacca cctgtggcag caaaagcacg cggtaaaaac ctcggccgag
     6121 ctgctcgaga cgatcaagga cgtctgcacg ccggagttct tcgccgacgc ggcggcgggg
     6181 ttttcgcgcg cgccgatggc tgtgcgcgtt tcaccgtacg cgatttcgct gatcgactgg
     6241 agcgacccga tcgccgaccc gatccgccgg cagttcgttc cgctcgcctc ggcgctgctg
     6301 cccgaccatc cgcggctcac gctcgactcg ctgcacgaac aagccgactc gccggttccc
     6361 ggcctggtgc atcgctacgt cgacaaagcg ctgttcttgc cgctcaacac gtgtccggtg
     6421 tactgccgct tctgcacgcg cagctacgcg atcggccccg ataccgaaaa cgtcgataag
     6481 gtcgcgcttg ccaagacgcc caaacagtgg gaagacgcgt tcaaatacgt cgccgagcgg
     6541 ccggagctcg aagacatcgt gatctcgggc ggcgacacct accagctgcc gcccaagaac
     6601 gtggaactca tcggcaacgc gctgctcgac atcccgcacg tgcggcggat gcgctttgcc
     6661 accaaagggc cggcgatcat gccgatgaag atcctcaccc ataccgaatg gctggacgcg
     6721 atcacgcgca tcgtcgaacg cggccgcacg atgggcaagg aagtggtgct ccatacccat
     6781 ttcaacgctc ccgaagagat tacgtggatc accgaaaagg cgatgcgaat gctcttcgag
     6841 cgcgggatat tcacgcgtaa tcagtcggtg ctcatccgcg gcgtcaacga cacccgcgag
     6901 cgaatgcagc tgctggtgaa acgcctcggc cacctcaacg tgcaccccta ctacgtctac
     6961 atgcacgaca tggtcaaggg agtcgaggag ctgcgcacga cgatccacac ggcgaccgac
     7021 accgagaagt tcgtacgcgg cagcaccgcc gggtttaaca cgccgacgtt cgtctgcgac
     7081 gcgcccggcg gcggcggaaa gcgcgacgtg cactcctacg aatactacga ccgggagaac
     7141 ggcatcgccg tttacgccgc gcccagcgtc aaaccgggta aggcgtttct gtacttcgat
     7201 ccgatcgatc ggctttcgcc cgacgcgcaa gcgcgctggg cggtgc
//
LOCUS       DASUCJ010000783         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_695851, whole genome shotgun sequence.
ACCESSION   DASUCJ010000783 DASUCJ010000000
VERSION     DASUCJ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695851"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /gene="mutS"
                     /locus_tag="VFU90_09390"
     CDS             complement(<1..2178)
                     /gene="mutS"
                     /locus_tag="VFU90_09390"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5480034.1"
                     /translation="MSGSAAKYSPMLEQYFGMKNKHPEAILLSRVGDFYEAYGEDAET
                     IARALQIALTSKEAGAGRRVAMAGVPHHALAQYLARLVAQRFIVALAEQLEVPQPNKL
                     VRRDVVRLVTPGTLIEEQLLDGKQNNYLAAIAAIEGTFALAYADVSTGFCAATALSGE
                     ESYEELLAELGRVGPSEIVADLPADVRATMATAIESLGARLASPPLGLVESDPREVLD
                     GFSHDESLAIARALEALASFVKRTGVANVLRRAHDAHAALKRPELYRRQTFLALDPST
                     RKHLELTRSQGQNPRATLLATLDRCATSMGSRMLARWISAPLVDRPMLAARQDAVAVL
                     LEEHARREALRELLKGTFDLERIAQKVRFRRALPRDLASLRRTLEGLDPLRRIAPPAL
                     VPLIERIADFAELYEDLQRTLVDEPPAQLGDGGVIRPGVDAELAECVNLRTDGRSKLS
                     ELEERERARTGIKTLRIKYASAFGYAIEVGRSHAGTVPADYVRKQTLTNGERYVTPEL
                     KELELAISTAQSRQERLEQTLFDGLVERVAARTDDLLRAAEAIAEIDVLAALALCAAE
                     RGYVRPAFVDESTLAIEEGRHPVMEAILQTSFVPNDLALRDDAHRFILLTGPNMGGKS
                     TYLRQAGLLTIMAQIGSFVPAKAMTLGPVDRIFTRIGAGDDLSSGQSTFYMEMAEAAN
                     ILRRSTHRSLLLIDEVGRGTGTLDGLSIAQAICEFLLGQERRSP"
     gene            complement(2343..>2833)
                     /locus_tag="VFU90_09395"
     CDS             complement(2343..>2833)
                     /locus_tag="VFU90_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480035.1"
                     /translation="PKSIISFDADSAGADPHVDLPNYSGEDRLILREAGTAIDYVNVM
                     DYDQFGWKPSTNPNCTYAPGSPDDCYLDIMREFANVPMRGGGTFPASKIVMGLMIGEA
                     DDRAFLKPGDAAGYATWVVENGYRGIMIWDLDLDNPQTPMNGTGYPKGTYVKAIGSAL
                     GK"
BASE COUNT          458 a          913 c          950 g          512 t
ORIGIN      
        1 cggcgaacgc cgctcctggc cgagcaagaa ttcgcagatc gcctgggcga tcgagagccc
       61 gtccagcgtt ccggttccgc gcccgacttc gtcgatcaac agcagcgagc gatgggtgct
      121 gcggcgtaaa atgttcgccg cctcggccat ctccatgtag aacgtcgact gtcccgacga
      181 caaatcgtcg cccgccccga ttcgcgtaaa gatgcgatcg acgggtccga gcgtcatcgc
      241 cttggccgga acgaacgagc cgatctgcgc catgatcgtc agcaacccgg cctgacgaag
      301 ataggtcgac ttacccccca tgttcggccc cgtcaacaga atgaagcggt gcgcgtcgtc
      361 gcgcagggca agatcgttgg gaacgaagct cgtctgcagg atcgcttcca tgacgggatg
      421 ccggccttct tcgatggcga gcgtgctctc gtcgacgaac gccgggcgca cgtagccgcg
      481 ctcggcggcg cagagcgcaa gcgcggccag gacgtcgatt tcggcgatgg cttcggcggc
      541 gcgcaacagg tcgtcggtgc gggcggctac gcgttcgacc agcccgtcga agagcgtctg
      601 ctcgaggcgc tcttggcgcg attgggcggt cgaaatcgcg agctcgagct ccttgagttc
      661 cggcgtgacg tagcgctcgc cgttggtaag cgtctgtttg cgaacgtagt ccgcgggcac
      721 ggttccggcg tggctgcggc cgacctcgat cgcgtatccg aacgcgctcg cgtatttgat
      781 ccgcagcgtt ttgatgccgg tgcgggcgcg ctcgcgttcc tccagttcgg agagtttgga
      841 gcgcccgtcc gtgcggaggt tcacgcattc ggcgagctca gcatcgacgc cggggcgaat
      901 gactccgccg tcgcctaact gcgccggcgg ttcgtcgacc agcgtccgct gcaaatcttc
      961 gtacagctcc gcaaagtcgg caatgcgctc gatcagcggt acgagtgcgg gcggtgcgat
     1021 ccggcgcagc gggtccaaac cttccagcgt gcggcgcagc gacgcgagat ctcgcggcag
     1081 cgcccggcgg aatcggacct tctgcgcgat ccgctcgaga tcgaacgtcc ctttcagcag
     1141 ctcgcgcagg gcctcgcggc gggcgtgttc ctcgagcagc acggcgaccg cgtcttgccg
     1201 cgccgccagc atcgggcgat cgaccagcgg tgccgagatc cagcgcgcga gcatccggga
     1261 gcccatcgag gtggcgcacc ggtcgagcgt cgccagcaac gtcgcgcgcg gattctggcc
     1321 ctgcgagcgc gtcagctcca gatgcttgcg cgtcgacgga tcgagggcga gaaacgtttg
     1381 ccgccggtac agttcgggac gcttgagggc ggcgtgagcg tcgtgagcgc ggcgcaggac
     1441 gttggccacg ccggtgcgtt tgacgaagga cgcgagcgct tcgagcgcgc gggcgatcgc
     1501 gagcgattcg tcgtgcgaaa atccgtcgag cacctcgcgt ggatcgctct cgaccagtcc
     1561 gagcggcggc gacgccagcc gcgcgccgag cgactcgatg gcggtggcca tggtggcgcg
     1621 gacgtccgcc ggcagatcgg caacgatttc agacggtccg acgcggccca gctccgcgag
     1681 cagctcttcg tacgactcct cgcccgacag ggccgtcgca gcgcagaagc cggtggagac
     1741 gtcggcgtaa gccagcgcga acgttccttc gatcgcggcg atggccgcca gatagttgtt
     1801 ctgtttgccg tcgagcagtt gttcttcgat caacgtgccc ggcgtgacga ggcgcacgac
     1861 gtcgcggcgc accagcttgt tgggctgcgg aacctcgagc tgctcggcga gcgcgacgat
     1921 gaatcgctgc gcgaccaatc gggccaaata ctgcgcgagc gcgtggtgcg gaacgcccgc
     1981 catcgcgacg cggcgtccgg ctccggcctc cttgctggtc aaagcaatct gcagcgcccg
     2041 cgcgatcgtt tcggcgtctt cgccgtacgc ttcgtagaaa tcgccgacgc gcgataggag
     2101 aatcgcctcg ggatgtttgt tcttcatccc gaaatactgc tccagcatcg gcgagtattt
     2161 cgcggcgctc ccgctcactc ggctaaagtg ccgaaccccg ccccgatttc cttcctagcc
     2221 ctagccgctc gaacgtgtca tcctgagcgg agcgagcgtt agcgagcgta gtcgaaggag
     2281 cgctcgacgt cgggccgtcc ttcgactgcg cgcctacggc gctccgctca ggatgacagc
     2341 gcttattttc ctaaggcgga gccgatcgct tttacgtacg tacctttggg atagccggtg
     2401 ccgttcatcg gcgtctgcgg attgtcgaga tcgaggtccc aaatcatgat gccgcggtaa
     2461 ccgttttcta cgacccaggt tgcgtagccg gccgcgtcgc ccggcttgag gaaggcgcgg
     2521 tcgtcggcct ccccgatcat caatcccata acgattttgc tggccgggaa ggtgccgccc
     2581 ccgcgcatcg gcacgttcgc gaattcgcgc atgatgtcga ggtagcagtc gtcgggcgaa
     2641 ccgggtgcgt acgtgcagtt cggattggtc gacggcttcc agccgaactg atcgtagtcc
     2701 atgacgttca cgtagtcgat cgccgtgccc gcctctctga ggatgagccg gtcttcgccc
     2761 gagtaattcg ggaggtcgac gtgcggatcc gcgccggcgg agtcggcatc gaaactaatg
     2821 atggatttcg gaa
//
LOCUS       DASUCJ010000784         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_833382, whole genome shotgun sequence.
ACCESSION   DASUCJ010000784 DASUCJ010000000
VERSION     DASUCJ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833382"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1483)
                     /locus_tag="VFU90_09400"
     CDS             complement(170..1483)
                     /locus_tag="VFU90_09400"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU5480036.1"
                     /translation="MHSTPHETRRPAGKRDILKLAREQNVRFVRLVFADIFGVSKNVS
                     VPAEELENALEGRVTFDGGSIDGFVRGEELDMLLKPDPSTFAVYPWAAPNGHEARLIC
                     DIAMPDGQPFEGCPRTTLRRAIESASGLRGLRVGLEAEFYLFAQQSGDAASTSTSDVG
                     SYFDFSANDRGEEARNAIVAALQSMGVPIASAHHEHGAGQHEIDLAHDDPLSAADHAL
                     TLRTIAKHVAAGFDLDATFMPKPLEDRAGSGLHVDFRLDDAADEATALYAVGGLLAHA
                     KAATAICNPTVNSYKRLVAAWDAPIYTVWSQRSANALVRVPPEQRPPRIEMRSPDPAC
                     NPYLALSVLVGAANDGIATATLPGPPLAGSTYQLNEEERRERGIGTLPKSLRQAIDEL
                     DADPVVRAALGDHLYHAFRDAKLEEYERYRRAVHPWEREQYLRLY"
     gene            complement(1464..>2002)
                     /locus_tag="VFU90_09405"
     CDS             complement(1464..>2002)
                     /locus_tag="VFU90_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5480037.1"
                     /translation="LLFVPAGREVDASNARTAAALLPKNAAKRHPQLRFSGGVGTVET
                     PLALHLSAERAEAALAIGRRVFGAGRVSAYDELGAYPLLYEGADVRRLETFARRVLEP
                     LREYDRKHHTELERTLRLFFAVGQNVKTAAAELSVHRHTVFYRLRQIGEICGCSLDNP
                     HDQLTLRLAVAIDALHSS"
BASE COUNT          311 a          650 c          708 g          333 t
ORIGIN      
        1 cgcacgacgc gcgccgacgt cgaacgcgcg tgggaaattt tgcggcgcga agcggagcgg
       61 ttggctgcag actagccgcc cttcgactgc gcggcctttg gccgctccgc tcagcccttc
      121 gactgcgctc ggtgagcgaa gctcacctcg ctccgctcag gatgacaggc taatacaatc
      181 gcaggtactg ttcgcgttcc caggggtgga cggcgcggcg gtagcgttcg tactcttcga
      241 gcttggcgtc gcgaaacgcg tgatagagat gatcgccgag ggcggcgcgt acgaccgggt
      301 cggcgtcgag ctcgtcgatc gcttggcgca gcgatttggg gagcgtgccg atgccgcgct
      361 cgcggcgttc ttcctcgttg agctgatagg tcgagccggc cagcggaggt cccggaaggg
      421 tcgcggtggc gatgccgtcg ttggccgcgc cgacgagcac cgagagcgcg agatagggat
      481 tgcaggccgg atccgggctg cgcatctcga tgcgcggcgg ccgctgctcc ggcggcacgc
      541 gcaccagcgc gttggcgctg cgctgcgacc agaccgtata gatgggtgcg tcccaggcgg
      601 cgaccagccg cttgtaggag ttcacggtgg gattgcagat tgcggtggcg gccttcgcgt
      661 gggccagcag cccgccgacg gcgtaaagcg cggtcgcttc gtcggcggcg tcgtccagcc
      721 ggaagtcgac gtgcaatccg ctgccggccc ggtcttccag cggcttcggc atgaacgtcg
      781 cgtccaaatc gaagccggcc gcgacgtgtt tggcgatcgt gcgcagggtc aacgcgtggt
      841 cggcggcgga aagcggatcg tcgtgcgcga gatcgatctc gtgctgcccg gcgccgtgct
      901 cgtggtgcgc cgaagcgatc ggaaccccca tcgactgcaa ggccgcgacg atcgcgttgc
      961 gggcttcttc gccgcggtcg ttggcggaaa aatcgaagta cgaaccaacg tcggacgtcg
     1021 acgtcgatgc ggcgtcgccg gattgctgcg cgaacagata aaactcggcc tcgaggccga
     1081 cgcgcagacc gcgcagcccg ctcgcgctct cgatcgcgcg ccgcagcgtc gtgcgggggc
     1141 agccttcgaa cggctgtccg tcgggcatcg cgatgtcgca aatcaaacgc gcttcgtgcc
     1201 cgttgggcgc cgcccacgga tagaccgcga aggtcgacgg atccggcttg agcagcatgt
     1261 cgagctcttc gccgcggacg aacccgtcga tcgagccgcc gtcgaaggta acgcggcctt
     1321 cgagcgcatt ttcgagctcc tcggccggga ccgagacgtt cttgctcacc ccgaaaatgt
     1381 cggcgaacac cagccggacg aagcgcacgt tctgctcgcg cgccagcttg aggatgtccc
     1441 gcttaccggc ggggcgccgc gtctcatgag gagtggagtg catcgatcgc taccgccaga
     1501 cggagagtga gttgatcgtg aggattgtcg agcgagcatc cgcagatctc gccgatttgg
     1561 cgcagccggt agaacaccgt gtggcggtgc accgaaagct ccgccgccgc cgtcttgacg
     1621 ttttgcccga ccgcgaagaa cagccgcagc gtgcgctcca gctcggtgtg atgtttgcga
     1681 tcgtactcgc gcagcggctc caggacgcgg cgcgcaaacg tttccaaccg gcgtacgtcg
     1741 gcgccttcgt acagcagcgg gtaggcgccg agctcgtcgt acgcgctcac gcgtccggcg
     1801 ccgaaaacgc gccgtccgat cgccagtgcg gcttccgcgc gctcggcgct cagatgcagc
     1861 gcgagcggcg tttcgaccgt cccgacgccg ccgctgaatc gcaactgggg atggcgcttg
     1921 gcggcgttct tcggcagcag cgcggcggcc gtacgggcgt tgcttgcgtc gacctcgcgg
     1981 cctgccggaa cgaacagcaa ca
//
LOCUS       DASUCJ010000785         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1292096, whole genome shotgun sequence.
ACCESSION   DASUCJ010000785 DASUCJ010000000
VERSION     DASUCJ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292096"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="ctaD"
                     /locus_tag="VFU90_09410"
     CDS             <1..1437
                     /gene="ctaD"
                     /locus_tag="VFU90_09410"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEU5480038.1"
                     /translation="IFALTIRSELFEPGMQILRPERFNQLITLHGLIMIFGALMPGAV
                     GFANWMIPMQIGAPDMAFGRMNNLSFWLLPPAAIMLLIGLFVPGGAPETGWTLYPPLS
                     IQQGMGMDFTIFAIHIMGASSIMGSINIITTILNLRAPGMTLMKMPLFCWSWLITAYL
                     LIAVMPVLAGAVTMLLTDRHFGTSFFDAAGGGDPVLFQHVFWFFGHPEVYIIILPAFG
                     LISEVIPTFSRKPLFGYASMVYALCAIAILSFLVWAHHMFTVGLPAAGILFYMYATMM
                     IAVPTGVKVFNWLATMWKGSITLEAPMLFALGFLFLFTTGGFTGLILAIQPVDVQFQD
                     TYYVVAHFHYVMVAGTLFAFFAATYYWLPKWTGHMYNETVAKWHFWGSFIFFNVAFFP
                     MHFLGLAGMPRRIPDYAQQFTNWNQVSTIGAFGFGLSQLIFLYAVVSCIRGGKPAPAK
                     PWEGATSLEWTLPSPAPYHSFPTPPVVQ"
     gene            1434..1661
                     /locus_tag="VFU90_09415"
     CDS             1434..1661
                     /locus_tag="VFU90_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480039.1"
                     /translation="MTVADAARVPREDDFEARRRAAARRTGFGLLLVALIFFFGFIAS
                     RLWLSPDVSITAVGAAVVVFVIVAIGRHLRK"
     gene            1658..>2243
                     /locus_tag="VFU90_09420"
     CDS             1658..>2243
                     /locus_tag="VFU90_09420"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5480040.1"
                     /translation="MSAPATPPQPSGASPRSGEDAPARRLARSNRRVVVRLAVFAVAM
                     FGFGYALVPLYYTICNALGLRELDRPDAIVNTQVDATRTVHVQFDTNVRSMGWQFTAL
                     ERTADVHPGAVTQAVFRVVNASGHAITGQAIPSFGPRLAGGYFRKIECFCFQRQTLAP
                     GETRLMPVVFVIDRDLPREVSTITLSYTFFEVEGT"
BASE COUNT          315 a          768 c          711 g          449 t
ORIGIN      
        1 atcttcgcgc tcacgatccg ctccgagctc ttcgagcctg gaatgcagat cctgcgcccc
       61 gagcgcttca accagctcat cacgctgcac gggctgatca tgatcttcgg cgcgctgatg
      121 cccggcgccg tcggcttcgc caactggatg atcccgatgc agatcggcgc gccggacatg
      181 gcgttcggtc ggatgaacaa cctctccttc tggctgctgc cgccggcggc gatcatgctg
      241 ttgatcggcc tcttcgttcc cggcggcgcg ccggaaaccg gctggacgct ctatccgccg
      301 ctctccatcc agcagggcat ggggatggac ttcacgatct tcgcgatcca catcatgggt
      361 gcgtcgtcga tcatggggtc gatcaacatc atcacgacga tcctcaatct gcgcgcgccc
      421 ggcatgacgc tgatgaagat gccgctgttc tgctggtcgt ggctgatcac cgcctacctg
      481 ctgatcgcgg tgatgccggt gctggcgggc gccgtcacga tgctgctgac cgaccgccac
      541 ttcggcacct cgttcttcga tgcagccggc ggtggcgacc cggtgctgtt ccagcatgtg
      601 ttctggttct tcggacaccc cgaggtctac atcatcatcc tgccggcgtt cgggctcatc
      661 tccgaggtca taccgacctt ctcgcgcaag ccgctgttcg gctacgcgtc gatggtctac
      721 gcactgtgcg cgatcgcgat cctgtccttc ctcgtgtggg cgcaccacat gttcaccgtc
      781 ggcctgccgg cggcgggcat cctgttctac atgtacgcga cgatgatgat cgcggtgccg
      841 accggggtga aggtgttcaa ctggctggcg acgatgtgga agggctcgat cacgctggaa
      901 gcgccgatgc tgttcgcgct cggcttcctg ttcctgttca ccaccggcgg cttcaccgga
      961 ctgatcctcg cgatccagcc ggtcgacgtg cagttccagg atacgtacta cgtggtcgcg
     1021 cacttccatt acgtgatggt ggcgggaacg ctgttcgcgt tcttcgcggc cacctactac
     1081 tggctgccga agtggaccgg ccacatgtac aacgagaccg tcgcgaagtg gcacttctgg
     1141 ggctcgttca tcttcttcaa cgtggcgttc ttcccgatgc acttcctcgg gctcgccggc
     1201 atgccgcgac gcattcccga ctacgcgcag cagttcacga actggaacca ggtgtcgacg
     1261 atcggcgcgt tcggcttcgg cctgtcgcag ctgatcttcc tgtacgcggt ggtgtcgtgc
     1321 atccgcggcg gcaagccggc accggcaaag ccgtgggaag gcgcgacgtc gctcgagtgg
     1381 acgctgccgt cgccggcgcc gtatcactcg ttcccgacgc ccccggtcgt gcaatgacgg
     1441 ttgcggatgc cgcgcgcgtg cctcgcgaag acgacttcga ggcgcggcgc cgggctgcgg
     1501 cccggcgcac cgggttcggg ctgctgctcg tcgcgctgat cttcttcttc ggcttcatcg
     1561 catcgaggtt gtggctgtcc cccgacgtgt cgatcacggc cgtcggcgcc gcggtcgtcg
     1621 tgttcgtgat cgtcgccatc gggcgccacc tgcgcaaatg agcgccccgg cgacgccgcc
     1681 gcagccgtcc ggcgcatcgc cgcgcagcgg cgaagacgcg ccggcgcgcc ggctcgcgcg
     1741 gagcaaccgg cgcgtcgtcg tgcgtctcgc ggtcttcgcc gtcgcgatgt tcggtttcgg
     1801 ctacgcgctc gtcccgctct attacacgat ctgcaacgcg ctcgggctgc gcgagctcga
     1861 ccgtcccgac gcgatcgtca atacccaggt cgacgcgacg cgcaccgtgc acgtccagtt
     1921 cgacaccaac gtccgcagca tgggatggca attcaccgcg ctcgagcgca cggccgacgt
     1981 ccatccgggc gcggtgacgc aggcggtatt ccgcgtggtg aacgcgagcg ggcacgcgat
     2041 caccgggcag gcgatcccga gcttcggtcc gcgcctcgcg ggcgggtact tccgcaagat
     2101 cgagtgcttc tgcttccagc ggcagacgct cgctccaggc gagacgcgcc tgatgccggt
     2161 cgtgttcgtg atcgaccggg acctgccgcg cgaggtctcg acgatcaccc tttcctatac
     2221 gttcttcgaa gtcgaaggta ccg
//
LOCUS       DASUCJ010000786         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1353437, whole genome shotgun sequence.
ACCESSION   DASUCJ010000786 DASUCJ010000000
VERSION     DASUCJ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353437"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1427
                     /locus_tag="VFU90_09425"
     CDS             249..1427
                     /locus_tag="VFU90_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480041.1"
                     /translation="MNNTLIVGQIALSFVLLVSAGLVLKSFQKLLRTDLGFDPQRVTS
                     MTMALPPQRYGMADKTQNVVFTSALVERIRSVPGVTAAAAMFPAIYANDVNTDGYLVE
                     GQAPAAESSAATQTVQISATPGLIATLGMHLLYGRDFTSADQKSSVPVVIVDEELAHR
                     YWKGGEAIGKRIRMSGDTTWRTIVGEVESIRDENVATPARPHTFFPYAQNPGSRPTLA
                     VRTAGPTSAGTVAAIRRTIAELDASIALDNVGPLSNAISRSLEDRRLTELLLAGFAAA
                     ALALAVIGLYGVMALYVAGRQREFGVRAAIGAAPGRLVALVLREGATLAVVGLLIGGT
                     AAMLATRWKRSLLYDVSPNDPIVFAVLAATLLAVAVAACGVPAARAARSDPSRTLRAE
                     "
     gene            complement(1580..>1843)
                     /locus_tag="VFU90_09430"
     CDS             complement(1580..>1843)
                     /locus_tag="VFU90_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480042.1"
                     /translation="LSFRAVAGVWAAAFVWMAGGCLLNAWRCRRVHCYISGPVLLLGA
                     IFQGLVASGVTNVGPRLAGNAVNATLILALLSFVPEMVWKRYA"
BASE COUNT          327 a          583 c          630 g          303 t
ORIGIN      
        1 cacgctcggc gccgtggtgg cagccatgtt cgtcgttgca atattgtcgg cgtacgggcc
       61 ggcacggcgc gccgcccgcg ccgacccgat ggctgcgctg cggagcgagt agcgacttct
      121 gcctcacgcg tcgacattcg atgcgtcggc aggaactcaa ctcatgcgtt aggcggcaag
      181 gcaacgggat gcgacccctc gggagtctcg cgggcacgaa ggggagcgca caccgtgggg
      241 cgcgtcgatt gaacaacacg ctgatcgtgg gacagatcgc gctctcgttc gtgctgctcg
      301 tgtccgccgg gctggtgctg aagagcttcc agaagcttct ccggaccgac ctcggattcg
      361 atcctcaacg cgtgacatcg atgacgatgg cactgccgcc gcagcgctac ggcatggcgg
      421 acaagacgca gaacgtcgtg ttcacgtcgg cgttagtcga acgcattcgt tcggtccccg
      481 gcgtgaccgc cgcggccgca atgtttccgg cgatttatgc gaacgatgtg aatacggacg
      541 gatatcttgt ggagggccaa gcgccggccg ccgagtcgag cgccgcgacg cagaccgttc
      601 agatttccgc cacgcccggg ttaatcgcga cgctcgggat gcatctgttg tacggccggg
      661 acttcacgtc cgccgaccag aagagttcgg tgccggtcgt catcgtggac gaggagctgg
      721 cgcatcgcta ctggaagggt ggagaagcga tcggcaaacg gattcgcatg agcggggata
      781 cgacatggcg cacgatcgtc ggcgaggtcg agagcattcg cgacgaaaac gtggcgacgc
      841 cggcgcgccc gcacaccttc ttcccatacg cgcagaatcc gggaagtcgc ccgacgctcg
      901 ccgtgcgaac ggcaggcccg acgtccgcgg gcacggtggc cgcgatccga aggacgatcg
      961 ccgagctcga cgcgagcatc gcgctggaca acgtggggcc cttgtccaac gcgatcagcc
     1021 ggtcactcga ggaccgacgc ctaacggagt tgctactcgc cggattcgcc gcggcggcat
     1081 tggcgctggc ggtgatcggt ctctatggcg tgatggcgct gtacgtcgcg ggccggcagc
     1141 gcgagttcgg tgtccgtgcg gcgatcggcg ccgcgccggg tcgtctcgtc gcgctcgtgt
     1201 tgcgtgaagg agcgacgctg gccgtggtgg ggctcttgat cggcgggacc gcggcgatgc
     1261 tggccacgcg gtggaagcga tcgctcctgt acgatgtcag ccccaacgac ccgatcgtgt
     1321 tcgcggtgct cgcggcgacg ctgctcgcgg ttgccgtcgc ggcgtgcggc gttccagcgg
     1381 cgcgcgctgc gcggagtgat ccgtcgagga cgttgcgcgc cgagtgattc gccctcggtg
     1441 ctgatgtctc tcagcacgat cgtcgtcgcg ctcaacgcgc agctcttgcg acgagtgaag
     1501 gtataaatgt cgagacccaa gtcacgccga ctgttgttgg gcgtgagaag aggatcgaaa
     1561 tgctcaggca cggcgcgcgt caagcatagc gcttccaaac catctccggc acgaacgaca
     1621 gcagagccag aatcaatgtc gcattcaccg cgttgcccgc taggcgcggc ccaacgttgg
     1681 tcacgcctga cgccaccagt ccctggaaga tggcgccgag cagcaacacc gggcccgaga
     1741 tgtagcaatg cacacgacgg caacgccacg cgttgagcag gcagccgccc gccatccaga
     1801 cgaatgcagc ggcccaaacg ccagccacag ctcgaaatga aag
//
LOCUS       DASUCJ010000787         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_466955, whole genome shotgun sequence.
ACCESSION   DASUCJ010000787 DASUCJ010000000
VERSION     DASUCJ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466955"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFU90_09435"
     CDS             complement(<1..631)
                     /locus_tag="VFU90_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5480043.1"
                     /translation="MGRRAGGSGRRRLNPSAVTAVILTRDEERNLPRALTSLPRGMHV
                     LVLDALSRDHTVEFARGAGAEVVQREWTNFVDARNFALAQVRTPWVLMLDADEALDDV
                     LREAIAGAPDDVDGYRVKRTTYYCGHPLRQWRDEPLLRLFRAGRARLQAHPAAGGSAA
                     VHESWSCSGPTGDLAGKLLHFSYPDRASYRAKYERYTATEAVGLRVSPGS"
     gene            complement(592..2277)
                     /locus_tag="VFU90_09440"
                     /pseudo
     CDS             complement(592..2277)
                     /locus_tag="VFU90_09440"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
     gene            complement(2340..3209)
                     /gene="prmC"
                     /locus_tag="VFU90_09445"
     CDS             complement(2340..3209)
                     /gene="prmC"
                     /locus_tag="VFU90_09445"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5480044.1"
                     /translation="MTVAATPSLAQALRSGIGTLQGGSETPRSDALLLLARALDCRRE
                     WILAHGEAPMNAPAAERFERWCRQRARGVPLAYLVGDAGFYGRTFAVDPRVLVPRPES
                     EHLIDEVLAFVARAARKRVEILDVGVGSGALACTLAAELPQADVCGTDRCAGALEVAA
                     ANARRLGVARRCALYAGDLAAPVAGRRFDAVLANLPYVPSNEIDAAAPLRFEPRGALD
                     GGPDGLDAYRRFVPAAPALLKPGGLLLLEAAPPQMAGLLELVRAAFAPVKPSVGVDYA
                     GLARYVRLETAAS"
BASE COUNT          519 a         1146 c         1065 g          552 t
ORIGIN      
        1 cgctccccgg cgaaacgcgc agcccgacgg cttcggttgc cgtgtagcgc tcgtacttgg
       61 cgcggtacga ggcgcgatcc ggataggaaa agtgcagcag cttccccgcc aagtcgccgg
      121 tcgggccgga acagctccag ctctcgtgca cggcggcgct cccgccggcc gcggggtgcg
      181 cctgcaggcg cgcgcgaccg gcgcggaaca gccgcagcag cggctcgtcg cgccactgac
      241 gcagcggatg cccgcaatag tacgtcgtgc gcttgacgcg atagccgtcg acgtcgtcgg
      301 gcgcgcccgc gatcgcttcg cgcagcacgt cgtcgagcgc ttcgtccgcg tcgagcatca
      361 acacccacgg cgtgcggact tgcgcgagtg cgaagttgcg cgcgtccacg aagttcgtcc
      421 attcgcgctg cacgacttcc gcgccggctc cccgcgcaaa ctcgaccgta tgatcgcgcg
      481 acagcgcgtc gagcaccagc acgtgcatgc cccgcggcaa actcgtaagg gcgcgcggca
      541 gattgcgttc ttcgtcccgc gtcaggatga ccgcggtgac cgccgacggg ttcagtcgcc
      601 tccgcccgga cccaccggca cgccggccca ctcgacgctc gccgctgcta cgccttcggt
      661 aagacccgcg tgcccgagcg cggcgccgat gaaatcgcga tacagcggcg atggacgatt
      721 cggacgcgac ttgaactcgg ggtgcgcttg cgtacccacg aaccacggat gcacgtcggc
      781 cggaagctcg atcacttcca cgagccgcgt cttgccgatc gtgtgatggc cggtaaaccg
      841 cataccgtgc tcttcgaaga tcgggcggta ccgattgttg aactcgtacc ggtggcgatg
      901 ccgctcgctg atttcgagcg ttccgtaggc gcgcgccgcc tggctgtccg gctcgagcac
      961 gcacgaatac gtaccgagcc gcatcgttcc gccgtagacc tccaagtttc gctggtcggg
     1021 catgaaatcg atcaccggat ccaggctggt ctcgtcgact tcgctcgtca tcgcttccgg
     1081 cagtccgcag acgttgcgcg cgaactcgat gcaggccagc tgcatcccat aacagatgcc
     1141 caagaatggg acgcgatgct cgcgagcgtg cgcgatcgcg cgcagcttgc ccttgacgcc
     1201 gcgcgcgccg aaccccggcg ctaccaagat cccgtgggca ccgcgcagca gctcgacgcc
     1261 ttcgtcctcg atcagctccg aatcgatcct ccggatatcc accgcggcgt tgtggaacgc
     1321 tcccgagtga tggagggctt cgacgatcga aatgtacgcg tctttgagct cgacgtattt
     1381 cccgaccagc gccaccgtta cgcgccgctg cgggtggagc atccgctcgg cgatcgcggc
     1441 ccactcttcc agcttcggcg ccgccgtcgg caggttgagc tagcgcaccg cggcctgtgc
     1501 cagaccctcg atctcgaggt tgagcggtac ctggtagatc gattcggcgt ccgagttctg
     1561 cacgaccgcg ctcggcggta cgtcgcaaaa caacgcaatc ttctccttca gctccaccgg
     1621 catcggcgcc gacgacgtgg tgcggcatac gatcgcgtcg ggcgatatcc cgatgccgcg
     1681 cagctcgcgc accgagtgct gcgtcggctt ggttttcagt tcgtcggcgg ctcccaagtg
     1741 cggtaccagc gtgaggtgcg cgtacgtcac gttctcttcg ccggcatcgt aacgcatttg
     1801 gcgaatcgct tccaaaaacg gcagcgactc gatgtcgccg accgtcccgc ctacttccac
     1861 gatacagacg tcggcgtgcc cggcttcggc gacgcgcttg atatgcgcct tgatctcgtt
     1921 ggtgatatgc ggaatcactt ggacggtcgc gccgagataa tcgccgcggc gttctttttc
     1981 gatcaccgaa ttgtagactt gtccggtggt gacgttgttg gcgcgctgca agttttcgtc
     2041 gatgaagcgt tcgtagtgcc ccaaatcgag atcggtctcg gcgccgtcct cggtcacgaa
     2101 cacctcgccg tgctggtacg ggttcatcgt gcccgcatcc acgttgatgt agggatctag
     2161 tttctggatc gaaacgctga tgccgcgcgc tttgaggagt cgtccgaggg aggctgccgt
     2221 gatgcccttg ccgagcgaac tcacgacgcc gccggtgaag aaaatgaact tcgccatctc
     2281 gggatgatat tgttccgacg ccgcggcgaa tccacctgcg atgccgcccg ccgccggcct
     2341 tacgacgccg cggtttcgag cctcacgtag cgggcaagtc cggcatagtc gacgccgacc
     2401 gacggcttca ccggtgcgaa cgccgcgcgt accagctcga gcagcccggc catttgcggc
     2461 ggcgcggcct ccagcagcaa caggccgccc ggcttcaaca gggcgggtgc ggccgggacg
     2521 aaccggcggt acgcgtcgag accgtcgggg ccgccgtcga gcgcgccgcg cggctcgaaa
     2581 cgcagcggcg ctgctgcgtc gatttcgttc gacggtacgt acggcaagtt tgcaagcacg
     2641 gcgtcgaacc gccggcccgc aacgggcgcg gcgagatcgc cggcgtacag cgcgcagcgg
     2701 cgcgcgacgc cgagccggcg ggcgttggcg gcggcaactt cgagcgcgcc ggcgcaccgg
     2761 tccgtcccgc aaacgtcggc ttgcggaagt tccgccgcga gcgtgcacgc gagcgcgccc
     2821 gagccgacgc cgacgtcgag aatttccacc cgtttccgcg cggcacgtgc gacgaatgcg
     2881 agcacttcgt cgatcaaatg ctcgctttcc gggcgcggca cgagcacgcg cggatcgacc
     2941 gcgaacgtgc ggccgtaaaa gcctgcgtcg ccgacgagat atgcgagcgg cacgccgcgc
     3001 gcccgttgcc ggcaccagcg ctcgaaccgc tcggcggccg gcgcgttcat cggtgcctcg
     3061 ccgtgcgcga gaatccactc tcgccggcaa tccagcgcgc gcgccagcag caacagcgcg
     3121 tccgaccgcg gcgtctcgct gccgccctgc agcgtcccga tgcccgagcg cagcgcctgc
     3181 gccaggctgg gcgtcgcggc aaccgtcatg cgggcgcgga ttcgcccgtc aacagacgcg
     3241 cgcgctcgtc ggcgagcagt tcgtcgacga tctgtcccag tt
//
LOCUS       DASUCJ010000788         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1475460, whole genome shotgun sequence.
ACCESSION   DASUCJ010000788 DASUCJ010000000
VERSION     DASUCJ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475460"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU90_09450"
     CDS             <1..297
                     /locus_tag="VFU90_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480045.1"
                     /translation="LALKPAQAPTFYRVIFASLAAGAGLALLPIPTISFLFWVSVVAG
                     LVTPATLALMLLVARDSRTMRGRPIGRWTAFWGWVTTGIVALAALAFLVANLRA"
     gene            300..>1177
                     /locus_tag="VFU90_09455"
     CDS             300..>1177
                     /locus_tag="VFU90_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5480046.1"
                     /translation="MDTTRLVRFARVLPVLLVLAAGCTRSGGTVPPGPPHVLRIAYAG
                     DPASLVPLVAIDQEIIALDTLFCQPLIGLDARNRQVPLLVTAIPSRQNGGISQDGTRI
                     TYHLRRGVRFADGVELTSADVAFTYRAIFDPRNRATSVEPYRRIASLQTPDPYTVVVR
                     LRKPWNAAVAVLFAEADYVYGILPKHAFADTKVVGTPWENAPFGTGAFRVKEWLRGDR
                     IVLVRNAYYRPRPKLEQIVLQIVSNLNSNFVTLRSGSADVGTLTADNVEQAARIPSLR
                     VMRVPENATGYLYLQTR"
BASE COUNT          172 a          401 c          390 g          214 t
ORIGIN      
        1 ctcgcgttga aaccggcgca ggcgccgacc ttttaccggg tcatcttcgc gtcgctggcg
       61 gccggcgcgg ggctggcgtt gttgcccatc ccgacgattt cgtttctgtt ttgggtgtcg
      121 gtcgtcgccg gcctcgtaac cccggcgacg ctggcgctga tgctgctggt cgcgcgcgac
      181 tcacgcacga tgcgcggccg cccgatcggc cgctggacgg ccttctgggg ctgggtgacg
      241 accggaatcg tcgcgctcgc cgcgctcgca tttctggtcg ccaacctgcg agcgtagcgt
      301 tggacacgac gcggctggtc cgcttcgcac gcgtactgcc ggtgctgctg gtgctggccg
      361 ccggctgcac ccgcagcggc ggaaccgtcc cgccgggtcc gccgcacgtg ctgcggatcg
      421 cgtacgccgg cgatccggca tcgctggtgc cgctcgtcgc gatcgatcag gaaatcatcg
      481 cgctcgatac cctgttttgc cagccgctga tcgggctcga cgctcgcaac cggcaggtgc
      541 cgctgctcgt taccgccatc ccgtcgcggc aaaacggcgg catctcgcaa gacggtacgc
      601 ggatcaccta ccatttgcgg cgcggcgtgc ggttcgccga cggcgtcgag ctgacgtcgg
      661 cggacgtcgc gtttacctac cgggcaatct tcgatccgcg aaaccgggca acgtcggtcg
      721 agccataccg ccggatcgct tcgctgcaga cgccggaccc gtacaccgtc gtcgtccggc
      781 tgcgcaagcc gtggaacgcg gccgtagccg tgctgttcgc cgaagcggat tacgtatacg
      841 gcattctgcc gaagcacgcg tttgcggata cgaaagtagt cggaacgccg tgggagaacg
      901 caccgttcgg caccggggcg tttcgcgtga aggagtggct gcgcggcgac cggatcgtgc
      961 tcgtccgcaa cgcctactac cgccctcgcc cgaagctcga gcaaatcgtc ctgcagatcg
     1021 tttcgaatct caactcgaac ttcgtcacgt tgcgttccgg cagcgccgac gtcgggacgt
     1081 tgacggcgga caacgtcgag caagcggcgc gaattccgag cctgcgcgtg atgcgcgttc
     1141 ccgaaaacgc cacgggatat ctgtacctgc agacgcg
//
LOCUS       DASUCJ010000789         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_298607, whole genome shotgun sequence.
ACCESSION   DASUCJ010000789 DASUCJ010000000
VERSION     DASUCJ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298607"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..645
                     /locus_tag="VFU90_09460"
     CDS             337..645
                     /locus_tag="VFU90_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480047.1"
                     /translation="MTKRRDTAFDNLAHHVDEILNAHGILRAVDEDAWCWQLGNYRVA
                     LGEDNTDRGYPVALFSLATEPNGQRSWRRDLATDRYVESPAAAEQLASAIERRLSTGT
                     "
     gene            816..1247
                     /locus_tag="VFU90_09465"
     CDS             816..1247
                     /locus_tag="VFU90_09465"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEU5480048.1"
                     /translation="MRATWKPHEKHGKLTMRSDLPSSVYAFPKQRKEPLTDARHVRNA
                     IARFDQVTDASGADRELAFANIKKAAKHYGVDLSESSAGELGVHPQRGRKARARKTGA
                     TRKRTGAARKAAKKAVATKRRTGTLRKAARKAAVTRKKKAS"
BASE COUNT          251 a          418 c          419 g          186 t
ORIGIN      
        1 aacgagcgac taagcgccag gtcagtcgca tagacggcgg gtttgaaggc gcccgccggc
       61 gggaaactcc ttcgtgcggg ggggtcatcg cgccgagagc aggaggccgg agaatggtac
      121 cgtcgtggct cctcgagacc gccgagctgt tgaagtgggt ttctttggcc gtcctggtgc
      181 tcaacctggc cgcgatcctg atccgctacg ctcactggta ggccgaaatt cttgccgcgg
      241 acgcccccgt tcttcgagga gcgaaagcgg gccgcggcgg agtagttctc cggacgaagc
      301 accggctttg agaatgggag aaccaccgaa atgcccatga ccaaacggcg tgataccgcc
      361 ttcgacaatc tcgcgcacca cgtcgacgaa atcctgaacg cgcacggcat tctccgggcc
      421 gtcgatgaag acgcctggtg ctggcagctc ggcaactatc gcgtggcact gggggaagac
      481 aacaccgatc gcggctaccc ggtcgccctg ttttcgctgg ccaccgagcc caacgggcag
      541 cggagctggc ggcgcgatct cgcaaccgac cggtacgtgg agtctccggc ggccgcggag
      601 cagctcgcga gcgcgatcga gcggcgcctt tcgaccggta cctaaagcgg ccgagcggga
      661 gcacaacgtt cgctcaggcg attacgttag gatggtggtc atcgtcgcgc gactcgcgcc
      721 cgaggtaccc caccccgtcg cccgtcatcc ccttttctgg cgacggggtg tcgtttgcgt
      781 ttacgccgca tcgccgtaag ggtagaggtt cccttatgag agcgacctgg aaaccccacg
      841 agaagcacgg caagctgacg atgcgcagcg acttaccgtc gagcgtgtac gctttcccca
      901 agcagcggaa agaaccgctc accgacgcgc gccacgtacg caacgcgatc gcccgcttcg
      961 atcaagtcac cgatgcatcc ggcgccgacc gggaactcgc attcgccaac atcaagaagg
     1021 ccgcgaagca ctacggcgtc gacttgagcg aatcgtcggc cggcgaactc ggcgtgcatc
     1081 cgcagcgggg acgcaaagcg cgtgcccgca agaccggcgc gacgcgcaag cggacgggcg
     1141 ccgcgcgcaa ggccgccaag aaagcggttg cgaccaagcg gcgcaccggg acgctgcgca
     1201 aggccgcacg caaagccgcc gtcacgcgca agaaaaaagc gtcgtaaccg gttacggctg
     1261 cggcgatccg ggcg
//
LOCUS       DASUCJ010000790         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_665460, whole genome shotgun sequence.
ACCESSION   DASUCJ010000790 DASUCJ010000000
VERSION     DASUCJ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665460"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..932)
                     /gene="ispE"
                     /locus_tag="VFU90_09470"
     CDS             complement(66..932)
                     /gene="ispE"
                     /locus_tag="VFU90_09470"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5480049.1"
                     /translation="MLPPLLAPAKINLTLEILARRDDGYHGVRSVMIPLDLSDEITVE
                     PGERFAFTCSDAALEGDDNLVMRALRALGDLPPRHVTLHKRIPVQAGLGGGSSDAAAI
                     LRAAMAGAFGPPPPADWPAVARALGSDVPFFLAGTGALVEGTGERVTPLGALPPWFVL
                     VVKPPVGVSTADAYARLDRRQRTVRPRNASASLALLAALQRGSFDAVEAGMHNDFQEP
                     VAEATPEVATALEALAAAGARRPLLAGSGACVFALAPTRADAGRMLERLRLPPDYARF
                     VTAFAPTPDWRR"
     gene            1005..3464
                     /locus_tag="VFU90_09475"
     CDS             1005..3464
                     /locus_tag="VFU90_09475"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5480050.1"
                     /translation="MGPSYDAQRNEFCAYVLHESQTPYGLLLGNLKAYAQSTAKGGVR
                     GLWDADTDQIIFGMHHIAYRLRDADRSGKTLFPHQIERDLTFLPYAQISEFTLNDRVH
                     VTEAFYVPRGPEFDRAVAFVVDVTLYNPGREALEVAVFPWAMLVGQRFYGEPEHEVRA
                     WNDGRLICSKNLETGGERWWGGSRQPVAVELSLREQALLESMRRATLLHDDSSLHLED
                     ITPEQAELVSRRIFGAFEYSVAVAPGARESMRLAVVYHKDGTEKSRPVLEALLKDPRA
                     LHDTQRYYAERLADARFMTPSPEISRGVAWAKANMLRIVKEYPHGWGSTNSPPSDILV
                     SRDTSWFVHGFDYFWPEFSRNALEVFNRAVDPSGLMIEYIRGVSGYKTAYDLNINDDT
                     PLHVIAMLHHYNATLDDAWVREHLPLVIKLIDYMLTQRDDRDLVFCKAKGVDMYGISS
                     WRNIIPYYTLDGAVTEINAECVYALEAAAMLCAVAGDNEHWQIYVTQAQKMREAMMAY
                     LFNDDTGAFVLNYDQDQNYQDNFTADEIFPVLFNVADAKERRAILQRLHEADFITPVG
                     LRTISTADAWYFPSYGFGLLGGVWPDLTLWYAVALARNGMTDEAVNFLDVTYKAMEGG
                     SPRNTVPGEFAEWFDGGSLSNRGMYLSPWTGAKYLWAVAETVGGLDGYRTSGRPHLAP
                     LRPKGWDWIAAARVHWGGKRRTYVIDLRNDAIYGDMPELSAEEPFTCIYAGRDVSDEV
                     TTSPVEVGAIAFEDDRGAVRIFLGNHLDKPRNVLVEFRGSTARVDMAAGELREVHLIG
                     TPSDRRARAAKLDVVPQAARV"
BASE COUNT          627 a         1198 c         1219 g          581 t
ORIGIN      
        1 ccccggctga cggcgggcga gcgcgtcttc cggccccccg gcgagtacga ccgctccgat
       61 ggggctcaac ggcgccagtc cggcgtcggc gcaaacgccg taacgaaacg cgcgtagtcc
      121 ggcggcagcc gcagtcgttc gagcatgcgg ccggcgtcgg cgcgcgtcgg cgccagcgcg
      181 aacacgcagg cgcccgaccc cgcgagcaac ggccggcgcg cgcccgccgc cgccagcgcc
      241 tcgagcgccg tcgcgacctc gggcgtcgct tccgcgaccg gctcctggaa gtcgttgtgc
      301 atcccggctt cgaccgcgtc gaagctgccg cgctgcagtg cggccagcag ggcgagcgaa
      361 gcggaggcgt tgcgcgggcg gaccgtgcgc tgccggcgat cgagtcgcgc gtaggcgtcc
      421 gcggtggaaa ccccgaccgg cggtttgacg accagtacga accacggcgg cagcgcgccg
      481 agcggcgtca cgcgctcgcc cgtgccttcg accagcgcgc cggttccggc cagaaagaac
      541 ggtacgtcgg agccgagggc gcgcgcgacc gccggccagt cggcaggcgg cggcggcccg
      601 aaggcgccgg ccatcgccgc gcgcaggatc gcggccgcat cgctcgatcc tccgcccaac
      661 cccgcctgca cgggaatgcg cttgtgcagc gtcacgtgcc gaggcggcaa gtctccgagc
      721 gcgcgcagcg cgcgcatcac gaggttgtcg tcgccctcga gcgcggcgtc gctgcaggtg
      781 aaggcgaagc gttcgcccgg ttcgacggtg atctcgtcgc tcaaatcgag cgggatcatg
      841 acgctgcgca cgccgtgata cccgtcgtcg cggcgcgcga ggatttcgag cgtaaggttg
      901 atcttcgccg gtgcgaggag cggcgggagc atcgtcgcgc cgtagttagc ggccgagcta
      961 ggaggcgcct tcgtcgcgaa cgccgcaaag gtaccgggca aatcatgggc ccgagttacg
     1021 acgctcagcg aaacgaattt tgcgcgtacg tcctgcacga atcgcagacg ccttacgggc
     1081 tgctgctcgg caatctcaaa gcgtacgcgc agtctaccgc caagggcggc gtgcgcggat
     1141 tgtgggacgc cgacaccgat cagatcatct tcgggatgca ccacatcgcg tatcgtttgc
     1201 gcgacgcgga ccgctccggt aagacgctgt ttcctcatca aatcgagcgc gacttgacgt
     1261 ttctgccgta cgcccagata tcggagttca ccctgaacga ccgcgtgcac gtcaccgagg
     1321 ccttctacgt gccccgcgga ccggaattcg accgcgcggt ggccttcgtc gtggacgtca
     1381 ccctctacaa tcccggcagg gaagcgctcg aggtcgcagt ctttccgtgg gcgatgctgg
     1441 tcggacagcg gttctacggc gagcccgagc acgaggttcg ggcctggaac gacggccgtc
     1501 tcatctgctc gaagaacttg gaaaccggcg gcgagcgctg gtggggagga tcccgtcagc
     1561 cggtcgcggt cgagctttcg ctgcgcgaac aagcgctgct ggaatcgatg cgccgcgcga
     1621 cgctgctgca cgacgattcg agcctgcatt tggaagacat cacccccgag caggccgagc
     1681 tcgtcagccg gcgcatcttc ggcgcgttcg agtactcggt cgccgtcgcg ccgggcgcgc
     1741 gcgaatcgat gcggttggcg gtggtctatc ataaagacgg aaccgaaaaa agccgccccg
     1801 tgctcgaagc gctgctgaag gatccgcggg ccctgcacga cacccagcgc tactacgccg
     1861 aacgcctcgc cgacgcgcgc ttcatgacgc cgtcgcccga aatcagccgc ggcgtggcct
     1921 gggcgaaagc caacatgctg cggatcgtca aagaatatcc gcacggctgg ggttcgacca
     1981 actcgccccc gtccgacatc ctcgtatcgc gcgacacctc gtggttcgtg cacggcttcg
     2041 attatttctg gccggagttc agccggaacg cactcgaggt gttcaaccgt gcggtcgacc
     2101 cgagcggcct gatgatcgag tacattcgcg gcgtgagcgg ctacaagacc gcctacgacc
     2161 tcaacatcaa cgacgatacg ccgctgcacg tcatcgcgat gctccaccac tacaacgcca
     2221 ccttggacga cgcgtgggtg cgcgagcact tgccgctggt gatcaagctg atcgactaca
     2281 tgctgaccca gcgcgacgac cgggatctgg tgttttgcaa ggccaaaggc gtcgacatgt
     2341 acgggatatc ctcgtggagg aacatcattc cgtactacac gctcgacggc gccgtaaccg
     2401 agatcaacgc cgaatgcgtg tacgcgctgg aagcggcggc gatgctgtgc gccgtggccg
     2461 gcgacaacga gcactggcaa atctacgtca cgcaagcgca gaagatgcgc gaagcgatga
     2521 tggcgtattt gttcaacgac gacaccggcg cgttcgtgct caattacgac caggaccaga
     2581 actatcaaga caatttcacc gccgacgaga tctttccggt gctgttcaac gtcgccgacg
     2641 ccaaagagcg gcgcgcgatt ctccagcggc tgcacgaagc cgatttcatc acgcccgtcg
     2701 ggctgcgcac catctcgacg gccgacgcgt ggtactttcc gtcctacggc ttcggcttgc
     2761 tggggggcgt gtggccggat ctcacgcttt ggtacgcggt cgcgctcgcc cgcaacggca
     2821 tgaccgacga agcggtgaat tttctggacg ttacgtacaa agcgatggag ggcgggagtc
     2881 cgcgcaacac cgtgcccggt gagttcgccg aatggttcga cggcggttcg ctcagcaatc
     2941 gcggaatgta tctctcgccg tggacgggag cgaaatatct ttgggcggtc gcggagaccg
     3001 tcggcgggct cgacgggtat cgcaccagcg gacgtccgca cttggcaccg ctgcgtccga
     3061 agggctggga ttggatcgcg gccgcgcgcg tgcattgggg cggaaagcgg cgaacctacg
     3121 tgatcgattt gcgcaacgac gcgatctacg gcgacatgcc ggaactttcg gcggaagagc
     3181 cgtttacgtg catttacgcg ggtcgcgacg taagcgacga ggtgacgacc tcgcccgtcg
     3241 aagtcggcgc gatcgcattc gaggacgatc gcggagcggt gcgcatcttt ctggggaacc
     3301 atctcgacaa gccgcgcaac gtgctggtcg agttccgcgg cagcaccgcg cgcgtcgata
     3361 tggcggccgg cgagcttcgc gaagtgcact tgatcggaac gccgagcgac cggcgcgccc
     3421 gtgccgccaa actcgacgtc gtcccgcagg cggcgcgggt ataatgggct tcgtgccccc
     3481 gctgaggctc gcgaagtgcc cgatcgctcc aaacgctttc tgaccgccgt cctggccgcg
     3541 ggttcgctcg tgctgctggt cgcgatcgcg atcggcgagc gcatgggcga ccgcgtcctc
     3601 gggcaggcga ccgagcagca gccgc
//
LOCUS       DASUCJ010000791         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_405259, whole genome shotgun sequence.
ACCESSION   DASUCJ010000791 DASUCJ010000000
VERSION     DASUCJ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405259"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VFU90_09480"
     CDS             complement(<1..1396)
                     /locus_tag="VFU90_09480"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5480051.1"
                     /translation="MTIHHPGCTKHLIRLASAALALAVAAGASVPVSAPAQVGTTTDI
                     LTGTVTDPLGHPIEDADVEAMSLETEISRHAHTNDKGRYTIVFPDGGGQYRVTVRFIG
                     MAPSVASVARQADEDRLVSDIQMSPTAQRLATVTVNARRNNRRGNQRTPGSTERDITS
                     EMAARMPVDASDLNALATLAPGVVGLDATDSTDAAFSVAGLRPTANSLTLDGLSFGSG
                     NGAVPQDAVRSTQVITSTYDVARGEFSGGLVASTTRGGTNVPEGSFTFTLRDQGLAWG
                     AGDSSLSAAYTQNQLGGGFGGPIVKDKLFVFGAVQGRWRDNSLPSLLNVNPAVLQRLG
                     VNADSLDRFLSLVGTAGVRPDVPDIPPTRSADNTLGLVRADWLISDNQTLMIRGDWRD
                     ITQNPTRVSQFALPQTGGVSDEGGGGVMGVLTSYFGGSFINELRAYWSRDHRAADPFM
                     DLPDGRVQVASQLAD"
     gene            1603..>1937
                     /locus_tag="VFU90_09485"
     CDS             1603..>1937
                     /locus_tag="VFU90_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU5480052.1"
                     /translation="MAGRLSNNRWLHGALTLGMVALVVYYARTVHWDASWRAIRSASP
                     WLLFLAALANLATLAAKAVVWWIFLRPVGAPSLGLAMRATAAGAGINNLLIANSGEAA
                     RAVLVTRSSA"
BASE COUNT          304 a          604 c          703 g          326 t
ORIGIN      
        1 tgtcggccag ctgcgaggcg acctgcacgc gaccgtccgg cagatccatg aacggatcgg
       61 cagcacgatg gtcgcgcgac cagtaggcgc gcagctcgtt gatgaagctg ccgccgaagt
      121 acgacgtgag cacgcccatc acgccgccgc cgccctcgtc ggacacgccg ccggtttgcg
      181 gaagcgcgaa ctggctcacg cgcgtcggat tctgcgtgat gtcgcgccag tcgccgcgaa
      241 tcatgagcgt ctggttgtcc gagatgagcc agtcggcgcg caccaggcct aacgtattgt
      301 cggcgctgcg cgtgggcggg atgtcgggga catcgggccg aacgccggcc gtgccgacga
      361 gcgagaggaa gcggtcgagc gaatcggcat tcacgcctaa ccgttgcagc acggcggggt
      421 tcacgttgag cagcgacggc agcgagttgt cgcgccaccg gccctgcacc gcgccgaaga
      481 cgaacagctt gtccttcacg atcgggccgc caaaaccgcc gccgagctgg ttctgcgtgt
      541 aggcggcaga gagcgatgaa tcgcccgctc cccacgcgag cccctgatcg cgcagggtga
      601 acgtgaagga gccttcggga acattggtcc cgccgcgcgt cgtggacgcc acgaggccgc
      661 cgctaaactc tccgcgcgca acatcgtagg tgctggtaat gacctgcgtc gagcggacgg
      721 catcctgcgg cacggcgcca ttgcccgaac caaagctgag gccgtcgagc gtcagggagt
      781 tcgccgttgg gcgaagaccg gcaaccgaga acgccgcatc ggtggagtcc gtggcatcga
      841 gaccgacgac gccgggcgcg agtgtcgcca gagcgttgag gtcgcttgcg tccaccggca
      901 ttcgcgcggc catctcggat gtgatgtcgc gttccgtgga tccgggcgtg cgctggtttc
      961 cgcggcgatt gttgcgccgg gcgttgacgg tgaccgtggc cagacgctgg gcggttggcg
     1021 acatctggat gtcgctcacc agtcgatctt cgtctgcctg gcgcgcgacc gaggcgacgg
     1081 aaggcgccat cccaatgaag cgcaccgtga cgcggtactg gccgccgcca tccgggaaga
     1141 caatcgtgta tcgtcccttg tcgttggtgt gcgcgtgccg cgagatctct gtctccaacg
     1201 acatcgcctc gacatcggcg tcctcgatcg ggtgcccgag gggatcggtg acggttcccg
     1261 ttaggatgtc ggtggtcgtt ccgacctgtg cgggagcgga cacggggacc gacgccccgg
     1321 cggcgacggc gagcgcaagg gcggccgacg ccaggcgaat caagtgcttg gtgcacccgg
     1381 ggtggtggat cgtcacgatg ggtcgggcat ccggttgggg ggcggggggc ggcgtcgaac
     1441 gtcccgtgag cgacacgtag tacccgggtc gatccggcga tgttgggtgg gggctgacca
     1501 ggcgtgaccg ggcagctcga gaaactgccg gcgaacgaat gacaatgcgg cgatgtcctg
     1561 ctagcgttca gcgcgctctt ccctcacgcg ttctcggatg tcatggccgg tcgactgagc
     1621 aacaaccgat ggctgcacgg tgccctgacg ttgggcatgg tcgcgcttgt cgtgtactac
     1681 gcgcgaacgg tccattggga tgcatcgtgg cgcgcgatcc gctcggcgtc gccgtggctg
     1741 ctgttccttg ctgcgcttgc gaacctcgca acgctggctg ccaaagcggt cgtgtggtgg
     1801 atattcctga gaccggtggg cgcgccgtcg ttgggcctgg cgatgcgtgc gacggcggcc
     1861 ggcgcgggca tcaacaatct gctcattgcg aacagcggcg aagctgcgcg cgcggtgctg
     1921 gtgacgcgca gctcggc
//
LOCUS       DASUCJ010000792         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_207343, whole genome shotgun sequence.
ACCESSION   DASUCJ010000792 DASUCJ010000000
VERSION     DASUCJ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207343"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..483
                     /locus_tag="VFU90_09490"
     CDS             10..483
                     /locus_tag="VFU90_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU5480053.1"
                     /translation="MFEPDGEGGWTAVHHPFTSPKPEYAESFHEHPESALAYAYDIVL
                     NGNEIGGGSIRIHRGEMQQRVFDVLGLTKDEAEAKFGFLLEAFQYGPPPHGGIALGLD
                     RLCALLAGVESIREVIAFPKTASGGDPLTGAPTTITAAQRKEAGIDAVVPPASPS"
     gene            complement(562..>1135)
                     /locus_tag="VFU90_09495"
     CDS             complement(562..>1135)
                     /locus_tag="VFU90_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480054.1"
                     /translation="TNAARTVLDLARALPFDAALVAAESALNLKLTTLAELDEVLAFC
                     IDWPGARKAGRVVAFASPYSESPGETLGRIAFDALGVPQPHQQVYIFDEFGLIARCDY
                     FWEQFHTVGEFDGKVKYVGAKARDNSVLLEKKREDRLRTAGLEVCRFEWAESRNRSES
                     VRHKAFSAFDRSANRQGERSYRFKLQPLAR"
BASE COUNT          190 a          385 c          376 g          184 t
ORIGIN      
        1 gacgcgccga tgttcgagcc ggacggagag ggcggctgga ccgccgtcca tcacccgttc
       61 acctcgccca agcctgagta cgccgaatcg ttccacgagc acccggagtc ggcgctggct
      121 tacgcctacg acatcgtgct caacggcaac gagatcggcg gtgggtcgat ccgtattcac
      181 cgcggcgaga tgcagcagcg ggtgttcgac gtccttgggc tgacgaagga cgaggcggag
      241 gcgaagttcg gcttcctgct tgaggcgttc cagtacgggc cgccgccgca cggcggcatc
      301 gcgcttggac tcgaccggct gtgcgcgctg ctggccggtg tcgagtcgat ccgcgaggtg
      361 atcgcgttcc cgaagaccgc gtcgggcggc gacccgctga ccggggcgcc gacgacgatc
      421 accgctgcac agcgcaaaga ggccgggatc gacgccgtcg tcccgcctgc gtcgcccagt
      481 tgatctccct agaccctgaa actcgcgcgt ggacacctcc accgcggtgt ccaagcgcga
      541 gtttcagggt ctaaggcgcg ctcagcgggc cagcggctga agcttgaagc gatagctgcg
      601 ctcgccctgc cggttggccg atcggtcgaa cgccgaaaac gccttgtgcc gcacggattc
      661 tgaccggttg cgcgactctg cccactcgaa ccggcacacc tctagcccgg cggtgcgcag
      721 ccggtcttcg cgctttttct caagcaggac ggagttgtcg cgcgccttcg cgccgacgta
      781 cttgaccttg ccgtcgaact cgccgaccgt gtggaactgc tcccagaagt agtcgcagcg
      841 ggcgatcagg ccgaactcgt cgaagatgta gacctgctgg tgtggctggg gcacgccgag
      901 cgcgtcgaac gcgatgcgcc cgagggtctc gccgggcgat tccgagtacg gcgaggcgaa
      961 tgcgacaact cggccggctt tgcgcgcgcc tggccagtcg atgcaaaacg ccaacacctc
     1021 atccaactcg gcaagcgtcg tcagcttgag attcagcgcc gactccgccg caaccagtgc
     1081 cgcgtcgaac ggcaacgcgc gtgccagatc aagcacagtg cgcgccgcgt tcgtc
//
LOCUS       DASUCJ010000793         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_467036, whole genome shotgun sequence.
ACCESSION   DASUCJ010000793 DASUCJ010000000
VERSION     DASUCJ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467036"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFU90_09500"
     CDS             <1..728
                     /locus_tag="VFU90_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480055.1"
                     /translation="GVLRARGLNSIMAVALAPPSGWLAAGSPAGLYMFPPDVGPGALV
                     RPGDVAAIAGGQRHMYVAVNDRIEIAPLSDDTARTWPGITMGSPVHALLLDTDHGLLW
                     ATTDRNLIALRISSDSLLRVGAAPLDAVGLHLAQTGDTIAVALGERGVRLFDVTDPKA
                     PRPVASWTTAHFAYDVSIDKQRLFVAAGPEGVYVVDLSSTPMRTLGLAHEMGFATALA
                     SRRGYTYILDRRGVVLRRVLSDF"
     gene            735..1445
                     /locus_tag="VFU90_09505"
     CDS             735..1445
                     /locus_tag="VFU90_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5480056.1"
                     /translation="MGLVRVAALATLVAACAVAGCGSSSSDRSSSPSASAAVASQPPA
                     SEPATKADTALDRAADHARVQGNANAPLWVVEISDFQCPFCREWHDQSYQAIKSQYVD
                     TGKIRFAYINYPLSIHKNAWPAAEAAMCAAAQGKFWPMHDSLFASQSRWENAPNPQPA
                     LDSIAASVGVSRDAYTSCVTQHRMRALIQADVDRANESGVTATPTFIIGTAKITGAQP
                     LDQFRAVVDAQLAKVKAQ"
BASE COUNT          230 a          534 c          534 g          270 t
ORIGIN      
        1 cgggagtgct ccgcgctcgc gggctcaaca gcatcatggc ggttgccctg gcgccgccga
       61 gcggctggct ggcggcgggc tcgccggccg gcctgtacat gtttccgccc gacgtcggcc
      121 ccggcgcgct ggttcgcccg ggcgacgtcg ccgccatcgc cggcggtcag cggcacatgt
      181 acgtcgccgt gaacgaccgc atcgagatcg cgccgttgtc cgacgacacg gcccggacgt
      241 ggccgggtat caccatgggg tcgccggtgc acgctctcct gctcgatacc gatcacggtc
      301 ttctgtgggc gacgaccgat cgcaacctca tcgcgctgcg catctcgagc gactcgctgc
      361 tgcgcgtcgg cgcggcgccg ctcgatgcag tgggcctgca cctggcgcag accggcgaca
      421 cgatcgccgt tgcgttaggc gagcggggcg tccggttgtt cgacgtcacg gatcccaagg
      481 cgccccgacc cgttgcgtcg tggaccaccg cccattttgc gtacgacgtc tcgatcgaca
      541 aacagcggtt gttcgttgcc gccggtccgg agggcgtgta cgtggtcgat ctgagcagca
      601 ctcccatgcg cacactgggt ctggcccacg agatgggctt cgcgacggcg ctcgcatcgc
      661 gtcgcggcta cacatacatt ctcgatcggc gcggcgtggt gttgcgccgc gttctttccg
      721 acttctgagg actcatgggt ctggtacgcg tggccgcgct cgcgacgctc gtcgcggcat
      781 gtgcggtggc cgggtgcggc tcctccagca gcgatcgctc gtcgtcccca tccgcatcgg
      841 cggcggtcgc ctcgcaaccg ccggcatcgg agccggcgac caaggccgat acggcgctcg
      901 atcgcgcggc ggaccacgca cgcgtgcaag gcaacgcgaa tgcgcccctg tgggtggtgg
      961 agatcagcga cttccagtgc ccgttctgcc gcgagtggca cgatcagagt taccaagcca
     1021 tcaagtcgca atacgtcgac acggggaaga ttcgctttgc gtacatcaat tacccgctct
     1081 cgattcacaa gaacgcatgg ccggccgccg aggcggccat gtgcgcggcg gcgcagggaa
     1141 agttctggcc catgcacgac tcgctgttcg cgtcgcagtc gcgttgggag aacgctccga
     1201 atccgcagcc ggcgctcgac tcgatcgccg cgagcgtggg cgtgtcccgc gacgcgtaca
     1261 cgtcctgcgt gacgcaacac cggatgcgcg cgctgatcca ggccgacgtg gaccgggcga
     1321 atgaatccgg cgtgaccgcg acgccgacgt tcatcattgg cacggccaag atcacgggcg
     1381 cccaaccgtt agaccaattt cgcgcggtgg tcgacgcgca gttggcgaag gtcaaggcgc
     1441 agtaagtcga cgtcgctttc acgccggcgc ggcacatgct tcgtccgttc tacgcgtttg
     1501 ccgtcgaagt cgcgcgcgtt gcggcgtcgg tggcgccgga gcgcgagggc aaattctggc
     1561 gatcgctc
//
LOCUS       DASUCJ010000794         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1093625, whole genome shotgun sequence.
ACCESSION   DASUCJ010000794 DASUCJ010000000
VERSION     DASUCJ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093625"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>1028
                     /locus_tag="VFU90_09510"
     CDS             44..>1028
                     /locus_tag="VFU90_09510"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEU5480057.1"
                     /translation="MRWWLALSGVLVAIGAASPARAQRIPRPNVQPARRDTTRRDTTA
                     ADSALAAKLKLSAPDSVMQALMRRPGYNTTRYEGERVTFDAQNDLFQILALQGKRAIV
                     QRGDSQTVFADTGVFFNQRTKVATATGNIILHDPTSGQADVVGRGRLEYSLNQRSATI
                     TNPMFAANMGQVWEIRALKGKSIFGDSAAGKGSAFYGLGGEITSCTDSIPDYHFTFKE
                     IKRTGSNTLVARPAILYIKDIPVMWLPFLFSDTRPGRHSGLLTPRFGISDFIRTSPSY
                     RRNVENVGYFLSINDYMDASAWIDWRSSAGATVGDPGWTKFNAEWRYSWLDR"
BASE COUNT          188 a          359 c          324 g          157 t
ORIGIN      
        1 cccgacgcga ggcggcgctg gcagcggaag agggagtgcc ggcgtgaggt ggtggctcgc
       61 cctgagcggc gtgctcgtcg cgatcggcgc ggcgtcaccc gctcgcgcgc agcgcatccc
      121 ccggccgaac gttcaacccg cgcgccgcga caccacgcgc cgcgacacca ccgccgcaga
      181 cagtgcgctg gccgcgaagc tcaagctctc ggcgcccgat tcggtgatgc aggcactcat
      241 gcgtcgtccg ggctacaaca cgacgcgcta cgagggcgag cgcgtcacct tcgacgcgca
      301 gaacgacctg ttccagatcc tcgccctcca gggcaagcga gcgatcgtgc aacgaggcga
      361 ctcgcaaacc gtgttcgccg ataccggcgt cttcttcaat cagcggacga aagtcgcgac
      421 cgccaccggc aacatcatcc tccacgaccc caccagcggc caggccgatg tcgtcggccg
      481 cggacggctc gagtacagtc tcaaccaacg ctcggcgacg atcaccaacc cgatgttcgc
      541 cgcgaacatg ggccaggtct gggagattcg cgcgctgaag gggaaatcca tcttcggcga
      601 ttccgccgcg ggcaagggat cggccttcta tggactcggg ggcgagatca cgagctgcac
      661 cgactccatc ccggactatc acttcacgtt caaggagatc aagcgcaccg ggtcgaacac
      721 cctcgtggcg cgaccggcga tcctgtacat caaggacatt ccggtgatgt ggctgccgtt
      781 tctgttctcg gatacgcggc caggccggca cagcgggctg ctcacgccgc gattcggcat
      841 ctccgacttc attcgcacca gtccgtccta ccggcgcaac gtcgagaacg tcggctactt
      901 cctctcgatc aacgactaca tggacgcctc ggcgtggatc gattggcgga gcagcgccgg
      961 cgcgaccgtt ggcgacccgg gatggacgaa gttcaacgcc gagtggcgct acagctggct
     1021 ggaccggt
//
LOCUS       DASUCJ010000795         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_971434, whole genome shotgun sequence.
ACCESSION   DASUCJ010000795 DASUCJ010000000
VERSION     DASUCJ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971434"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFU90_09515"
     CDS             <1..415
                     /locus_tag="VFU90_09515"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU5480058.1"
                     /translation="EKALKRASRLVKMLGAQPLRMTAAAHDCAVAAMSALPQLASIAL
                     TLAASDAAGLTASRRAASRLAGPGYKDATRIAMSPYDIWRPAIAANRREILRCLRALE
                     RSTARIARAARQADARAMERLFSKARSVRKRVVAG"
     gene            500..>1047
                     /locus_tag="VFU90_09520"
     CDS             500..>1047
                     /locus_tag="VFU90_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5480059.1"
                     /translation="MSSIARRIAAVAVAAACLAAYSPAAHADIVYYTPPSFKNRVSPV
                     YPESARAKHETGAVVLKVLVGADGKAKQFILFKSSGHKDLDDAVLAAAKASTYNPATR
                     GSTPVVGFYDVTYRFTLTGVAQDEGTSSTLTKKLAANPKDVATRLQVGNQYLLAKNYG
                     AAEQTYQAGTQLMPTNAKLWAN"
BASE COUNT          201 a          355 c          332 g          159 t
ORIGIN      
        1 ggaaaaggcc ctcaaaaggg cgtccaggct cgtcaagatg ttaggcgcgc aaccgttgag
       61 gatgaccgcg gcggctcacg actgtgccgt cgccgcgatg agcgcgttgc cgcagctggc
      121 ctcgatcgcc ctgacgctgg cagcatcgga cgcggccgga ctgacggcgt cgcggcgcgc
      181 ggcgtcgcgg ctcgcagggc caggctacaa agatgcgact cgcattgcga tgtcgccgta
      241 cgacatctgg cgaccggcca tcgcggcgaa ccggcgcgag atactccgct gtcttcgagc
      301 gctggagcgc tcgaccgcgc ggatcgcccg agccgctcgg caggctgacg cccgggcgat
      361 ggaacgacta ttctccaaag cccgatcggt gcgcaagcgg gtcgtcgcag gctagtcggc
      421 gaatcgtcca gccaccgttt tacgttcttc tcaacgcgcc gttcatctcg gcccgcacag
      481 aaaacccgca aggagtccaa tgagcagcat cgcaaggcgg atcgccgccg tcgccgtggc
      541 cgcggcttgc ttagccgcct actcgcccgc agcacacgcg gatatcgtct actacacgcc
      601 cccgagcttc aagaatcgcg tcagtccggt gtatccggaa tcggcgagag cgaagcacga
      661 gacgggtgcc gtcgtcctca aagtcctcgt cggcgccgac ggcaaggcca agcagttcat
      721 cctcttcaag tcgtccggtc acaaggacct cgacgatgcc gtgttggcgg ccgcgaaagc
      781 gtcgacgtac aacccggcga cgcgcggatc gacgccggtc gtcggcttct acgacgtcac
      841 gtaccgcttc acgctcaccg gtgtcgcaca ggatgaaggc acctcgtcga cgcttacgaa
      901 gaagctcgcc gcgaatccga aagacgtggc gacacggctc caggtcggca accagtatct
      961 cctcgcgaag aactacggcg ccgccgagca gacctatcag gccggcacgc agctgatgcc
     1021 gacgaacgcg aagctgtggg cgaatca
//
LOCUS       DASUCJ010000796         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_161193, whole genome shotgun sequence.
ACCESSION   DASUCJ010000796 DASUCJ010000000
VERSION     DASUCJ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161193"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="VFU90_09525"
     CDS             complement(<1..>1215)
                     /locus_tag="VFU90_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480060.1"
                     /translation="EVRRFVSRSANAGQAFSIDGSSRYRDTNARHSLVRGLLDVDEDE
                     MLSLEELAQAETRLRLRDANDDAIVTLAELDDTLAGDSQAMMTAMAYRNTPAAMRLGP
                     LADWDGVIFTLAELYLRHGELPEEGFPLTPSLAQDLDADGDGGLDREEIERLDAIEPH
                     VALEVRFGMSGDELPGIFVEHMAPSLGAAEEVAVNSAGGVLLNLAGVRMRFEFVDRMP
                     AGAVGPSAEEQLAALDADKNGYLEKKELEEKSPGMAAMFDDWDANADGKVYAKEIESF
                     NRGRRVPQATAIRIAANDDQDSLFTCLDADFDGRLSPRELRRAPERLSVLDADGDGKL
                     SLDEIPGGMTVQIERGATMSEAPRPYAAAPVETRAAEGPPWFVFMDSNRDAEISAREF
                     PGSREKFARLDA"
BASE COUNT          186 a          409 c          403 g          217 t
ORIGIN      
        1 cggcgtcgag ccgggcgaac ttctcgcggc tgccggggaa ttcgcgagcg ctgatctcgg
       61 cgtcgcggtt ggagtccatg aacacgaacc agggcgggcc ttcggccgcg cgagtttcga
      121 ccggcgcggc ggcatagggt cgcggggctt cgctcatcgt ggcgccgcgc tcgatctgca
      181 ccgtcatccc gccgggaatc tcgtcgagcg ataatttgcc gtcgccgtcg gcgtcgagca
      241 cgctcaatcg ctcgggcgcc cgtcgcagct cgcgcggact gagccggccg tcgaagtcgg
      301 cgtcgaggca cgtgaacagc gagtcctggt cgtcgttggc cgcgatgcgg atggccgtgg
      361 cttgcggcac gcggcggccg cgattgaagg attcgatctc cttggcgtag accttgccgt
      421 cagcgttggc atcccagtcg tcgaacatcg cggccatgcc gggcgatttc tcttcgagtt
      481 cttttttctc caaatagcca ttcttgtcgg cgtcgagcgc ggccagttgc tcctcggccg
      541 acggcccgac cgcgcccgcc ggcatgcggt cgacgaactc gaaccgcatc cgcactcccg
      601 cgagattcag caatacgccg cccgccgaat tgacggcaac ttcttccgcc gcgcccaagc
      661 ttggcgccat atgctcgacg aaaatccccg gcagttcgtc gcctgacatg ccgaagcgaa
      721 cctccagtgc gacgtgcggc tcgatcgcgt cgagccgttc gatttcctcg cgatccaggc
      781 ctccgtcgcc gtcggcgtcg aggtcttggg ccaaggaagg cgtgagcgga aaaccttcct
      841 cgggcagctc gccgtggcgc aggtaaagct cggccagggt gaaaatcacg ccgtcccaat
      901 cggccagcgg accgagccgc attgcggccg gcgtgttgcg ataggccatc gcggtcatca
      961 tggcttgcga gtcgccggcc agcgtgtcgt cgagttcggc caaggtgacg atcgcgtcgt
     1021 cgttcgcatc gcgcagccga agccgcgttt cggcctgcgc cagttcctcg agcgagagca
     1081 tttcgtcttc gtcgacgtcg agcaagccgc gaacgagcga atgccgggcg ttcgtgtcgc
     1141 gatagcgcga cgagccgtcg atcgaaaacg cctgcccggc gttggccgag cgcgagacga
     1201 accgccgcac ctcgg
//
LOCUS       DASUCJ010000797         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_879926, whole genome shotgun sequence.
ACCESSION   DASUCJ010000797 DASUCJ010000000
VERSION     DASUCJ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879926"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..>1246
                     /locus_tag="VFU90_09530"
     CDS             263..>1246
                     /locus_tag="VFU90_09530"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEU5480061.1"
                     /translation="MLASLFGASRARAAITVYPRVGNQADSAITPQGPGLVIMGGGSD
                     VDGAFVWMHDTVVGAKSTSGADVVILRATGDNAYDSYIAGLAPFNSVQTLLIPTPASA
                     SDLSTAAAIVDKAEVVFFAGGNQADYVGWKGSPLLTAVAGCHQRGGVVGGTSAGAAIL
                     GQFVFDAVAAGSSNVATADAVANPYESTISFTHDLLPFAILDDAITDPHFVVRDRMGR
                     LTVFMARQLADGLASGTTHGIGVDEGTAVCIDKHGLGVIMQYTAGTGSAFVVSGGAAA
                     TIQAGTPLRYPALTVTRLDQQGQTYDFSHWCGTTTTYALSVDGTAMPPYSPA"
BASE COUNT          192 a          431 c          406 g          217 t
ORIGIN      
        1 actgtagcat ggctcttcgc tcgcgattcg ccgtcgggcc gagggctacc gtgcgagccc
       61 ctctcagcgt tggctgtcgc cgacgcccgc gccgggctca gcttctctcg taggtccggt
      121 accgcttcgg acctcgcgtg ctcgcgggat tgacgttaag tgttttctta aactactgct
      181 gcggggcatt ccaaccgcac ctatgacgga gcgatccacg atgccgccag gaagcttcac
      241 acgctcgctc agcaccttgt tgatgctcgc gtcgctcttc ggggcgagcc gcgcgcgcgc
      301 cgccatcacc gtctatccgc gcgtcggcaa tcaggcggac agtgcgatca cgccgcaggg
      361 cccgggcctc gtcatcatgg gcggcggcag cgacgtcgac ggcgccttcg tgtggatgca
      421 cgacaccgtc gtcggcgcca agagcacgtc gggtgccgac gtcgtgatcc tgcgcgccac
      481 cggcgacaac gcctacgact cgtacatcgc ggggctcgcg ccattcaact cggtccagac
      541 gctgctcatc cccacgccgg cgagcgcgag cgatttgagc acggcggccg ccatcgtcga
      601 caaggcggag gtcgtgttct tcgcgggcgg caaccaggcc gactacgtag gctggaaggg
      661 atcgccactt ctgaccgcgg tggcgggttg ccatcagcgc ggtggcgtcg tcggcggcac
      721 gagcgcgggt gcggcgatcc tcggccaatt cgtcttcgat gccgtcgccg ccggatcgag
      781 caacgtcgct accgcggacg cggtcgccaa tccgtacgaa tcgacgatca gcttcacgca
      841 cgacctgttg ccgttcgcga tcctcgacga tgcaatcacg gatccacact tcgtcgttcg
      901 cgatcgcatg ggacggctga ccgtcttcat ggcgcggcag ctcgccgacg gccttgccag
      961 cggcaccacg cacggcatcg gcgtcgacga aggcacggcg gtgtgcatcg acaagcacgg
     1021 tctcggcgtg atcatgcagt acacggccgg gacgggcagc gcgttcgtcg tcagcggcgg
     1081 cgctgccgcg acgatccagg ccgggacgcc gttgcgctat ccggcgctga ccgtgacgcg
     1141 gctcgaccag caagggcaga cgtatgattt ttcgcactgg tgcggaacga cgacgaccta
     1201 tgccctttcc gtcgacggaa cggccatgcc gccttattcg cccgcc
//
LOCUS       DASUCJ010000798         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_222517, whole genome shotgun sequence.
ACCESSION   DASUCJ010000798 DASUCJ010000000
VERSION     DASUCJ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222517"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2701)
                     /locus_tag="VFU90_09535"
     CDS             complement(<1..2701)
                     /locus_tag="VFU90_09535"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5480062.1"
                     /translation="MSHRRLQLAAAVVLCASTPFAFAATKLLRFPDVCGDRVVFTYAG
                     DLWTASTQGGTATRLTAGPGLEQSARFSPDCKTIAFTGEYGGDDQVYTIPAYGGEPKQ
                     LTFYPSKGPLPQRWGFDSQVYGWTPDGKSVLFRSYQDSTSESNPHLYTVPVEGGEPVM
                     LPMPTAGVGRYSPDGSQIVYSPKFRDFRTWNRYVGGWAQDLYIYDFAAKSAKNITNDP
                     NTDRDPVWIGNGIYFLADRGEHLNLYRYDTASGETKQLTDYKGQDARWASGDGKGQIA
                     FEVDGVLHVYDTHANTDKALDITVPSDLVRTRAQEVSVKDHVEDFSLSANGKRAVFTA
                     RGELFSVPLKDGITLDLTHSPGVHEREANWSHDGKHVAYVSDEGGEEAVWVRDADGGN
                     AKKLTTQTIGRLYAPRWSPDGAHIAFVDSDNNLRIVPANGGSAPVVAHDPSISRRDYA
                     WSPGGHYLAYTLTDKDTQQSQLYVRDIAAGKDVHLGQPRFDAYGPAFSPDGKYLYYLG
                     DREWTPQLSGVEWDFAANRDTGIFALALRKDVENPFAPRNDSASAGEKKGDAGKKDTD
                     KGKEKDKAKSPAQVNDRIDFDGLDQRVVRAPIEPDNIAGIAITKKAILYVVGDAPYYG
                     REGAFKTKLVSWSFEERKSSDVFAGVGDLSISDDGGTALVKSDGGYKWIDLNAGKPEA
                     KDVKTDGLFALVDPKAEYAEIFGEVWRRYRDYYYARNMHGYDWNAIRAKYEPMLAWVG
                     DRTDLNYVLGQMVAELSSGHAYVSGGDIGLPKKPHVGLIGARFALDAASGQYRIAKIM
                     QGQNDEERYRSPLTEVGIDVKQGDYVVAINGQPLTAHDNPYRLLRTAPGQLVQLTVNA
                     KPGSAGARTVLVKPIDDELPLNYLAWTEHNRAYVAKASNGEIGY"
     gene            2828..3175
                     /locus_tag="VFU90_09540"
     CDS             2828..3175
                     /locus_tag="VFU90_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480063.1"
                     /translation="MSVQRYYLSIDDLARARGQINELSFQGNSPDSFAALFQSALREP
                     TLWRRWRAMQPDPDAVDPALGASDPQATVSAEQADMHTDIVVTTSLPHAILKHRLTLL
                     VGAHWSLRDVKTA"
     gene            3229..>3731
                     /locus_tag="VFU90_09545"
     CDS             3229..>3731
                     /locus_tag="VFU90_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480064.1"
                     /translation="MFRIRLLPVAMTAVLAACAQAPQKPVPAKAAAAAPASQHVNPFL
                     AASTLPFQAPPFDKITDADYQPAIEEGMRQQIAEVDKIANDPSPPTFENTYVALEKTG
                     TLLYRVMAVFNGVSGANTDDTLQKLQETEAPKLAAHQDAIYLNGKLFARVDAVYNERA
                     KLKLDPES"
BASE COUNT          591 a         1205 c         1236 g          699 t
ORIGIN      
        1 cataaccgat ttcgccgttg ctggccttgg cgacgtacgc gcgattgtgc tcggtccagg
       61 cgagatagtt gagcggcagc tcgtcgtcga tgggttttac cagcaccgtg cgcgcgccgg
      121 cgctgccggg cttggcgttg acggtgagct gcacgagctg ccccggcgcc gtgcgcagca
      181 ggcggtacgg gttgtcgtgc gcggtcagcg gctggccgtt gatcgcgacg acgtagtcgc
      241 cttgcttgac gtcgataccg acctcggtga gcggtgagcg ataacgctcc tcgtcgttct
      301 ggccctgcat gatcttggcg atgcggtatt ggccgctcgc cgcgtcgagt gcgaagcgcg
      361 caccgatgag accgacatgc ggtttcttcg gcaggccgat gtcgccgccg ctgacgtagg
      421 cgtgcccgct cgacagttcc gcgaccatct ggccgagcac atagttgagg tcggtgcggt
      481 cgccgaccca cgccagcatc ggctcgtact tggcgcggat cgcgttccag tcgtagccgt
      541 gcatgttcct ggcgtagtag tagtcgcggt agcgacgcca cacttcgccg aagatttcgg
      601 catattcggc cttcggatcg acgagcgcga acaggccgtc ggtcttgacg tccttggcct
      661 cgggcttgcc ggcgttgagg tcgatccact tgtagccgcc gtcgctcttg accagcgcgg
      721 tgccgccatc gtcggagatc gacagatcgc cgacgcccgc gaacacgtcg ctgctcttgc
      781 gctcctcgaa cgaccagctc acgagcttgg tcttgaacgc gccttcgcgg ccgtagtacg
      841 gcgcgtcgcc gacgacgtag aggatggcct tcttcgtgat cgcgatgccg gcgatgttgt
      901 ccggctcgat cggcgcgcgc acgacgcgct ggtcgagacc gtcgaaatcg atgcggtcgt
      961 tgacctgcgc cggcgatttc gccttgtcct tttccttgcc cttgtcggtg tccttcttgc
     1021 cggcatcgcc tttcttctca cctgcggagg cgctgtcgtt gcgcggcgcg aacggattct
     1081 cgacgtcctt gcgcaatgcg agcgcgaaga tgccggtgtc gcggttcgcg gcgaaatccc
     1141 attcgacgcc cgacagctgc ggcgtccact cgcggtcgcc gaggtagtac aggtacttgc
     1201 cgtcggggga gaacgccggg ccataggcat cgaagcgcgg ctgacccaag tgcacgtcct
     1261 tgccggcggc gatgtcgcgc acgtaaagct gcgattgctg ggtgtccttg tcggtcaggg
     1321 tgtaggcaag gtagtggccg cccggcgacc aggcgtaatc gcggcgggaa atcgacggat
     1381 cgtgcgcgac gacgggcgcg gaaccgccgt tggccgggac gatgcgcagg ttgttgtcgc
     1441 tgtcgacgaa ggcgatgtgc gcgccgtccg gcgaccagcg cggtgcgtac aggcggccga
     1501 tggtttgcgt ggtgagcttc ttcgcattgc cgccgtccgc atcgcgcacc cagaccgcct
     1561 cttcgccgcc ttcgtcggag acgtaggcga cgtgcttgcc gtcgtgcgac cagttcgcct
     1621 cgcgctcgtg cacgccgggc gagtgcgtga ggtcgagcgt gatgccgtcc ttgagcggca
     1681 cgctgaacag ctcgccgcgc gcggtgaaca ccgcacgctt gccgttcgcg ctgaggctga
     1741 aatcctcgac gtgatccttg acgctgacct cttgcgcgcg cgtgcgcacg aggtcgctgg
     1801 gcacggtgat gtcgagggcc ttgtccgtgt tcgcatgggt atcgtagacg tgcagcacgc
     1861 cgtcgacctc gaatgcgatc tggcccttgc cgtcgccgct cgcccagcgg gcgtcctggc
     1921 ccttgtagtc ggtgagctgc ttcgtctcgc cgctcgccgt atcgtagcga tacaggttca
     1981 agtgctcgcc gcggtcggcg aggaagtaga tgccgttccc gatccacacc gggtcgcggt
     2041 cggtattcgg gtcgttcgtg atgttcttcg ccgacttcgc ggcaaagtcg tagatgtaca
     2101 aatcctgcgc ccagccgccg acgtagcggt tccaggtgcg gaagtcgcgg aacttcggcg
     2161 agtagacgat ttgcgaacca tcgggcgagt agcggccgac gccggcggtc ggcatcggca
     2221 gcatgaccgg ttcgccgcct tcgaccggca ccgtgtacaa gtgcggattc gactcgctgg
     2281 tcgagtcctg gtacgagcgg aacagcaccg acttgccgtc cggcgtccag ccgtagacct
     2341 ggctgtcgaa tccccagcgc tgcggcagcg gacccttgga cggatagaac gtgagctgct
     2401 tcggctcgcc gccgtaggca ggaatcgtgt agacctggtc gtcgccgccg tattcgccgg
     2461 tgaatgcgat cgtcttgcag tccggcgaga agcgcgccga ttgttccagg cccggacccg
     2521 cggtcaagcg cgtcgcggtg ccaccctgcg tcgaagcggt ccacaggtcg cccgcgtagg
     2581 tgaacacgac gcgatcgccg cagacatcgg gaaagcgcag caacttggta gcggcgaacg
     2641 cgaatggcgt cgacgcgcaa agcacgacag cggcggcaag ctgcaggcgg cggtgggaca
     2701 tgggcatctt ccagcggacg aaaggtggcg gattctagcg tccgccggcg cgcgccggca
     2761 ggggcgaaag tcacgggcct gcacgcgttc ggctctgcta gacttgcagg ccgatcgagg
     2821 agatttcatg tcggtgcagc gttattactt gtccatcgac gacctggccc gcgctcgcgg
     2881 ccagatcaac gagttgtcgt tccagggcaa ttcgcccgac agctttgccg ccttgttcca
     2941 atcggcattg cgcgagccca ccttgtggcg gcgctggcgc gccatgcagc cggatccgga
     3001 cgccgtcgat cctgcgctgg gcgcgagcga cccgcaggcc acggtcagcg ccgaacaagc
     3061 cgacatgcac accgatatcg tcgtcactac cagcctgccg catgccatcc tcaaacaccg
     3121 cctgaccctg ctggtcggtg cgcactggag tttgcgcgac gtcaagacag cgtgacgccg
     3181 cgtggacacg ttgctttttc cattcgtata cttcgtggag ttcctgccat gttccgaatc
     3241 cgtttgctgc ccgtcgccat gaccgccgtg ctcgccgcct gcgcgcaagc gccacaaaag
     3301 cccgtgccag ccaaggccgc cgcggcggcg ccggcgtcgc aacacgtcaa tccgttcctg
     3361 gcggccagca cgttgccttt ccaggcgccg cccttcgaca agatcaccga cgccgactac
     3421 cagccggcga tcgaggaagg catgcgccag cagatcgccg aggtcgacaa gatcgcgaat
     3481 gatccttcgc cgccgacctt cgagaacact tatgtggcgc tggaaaagac cggcacgctg
     3541 ctgtaccgcg tgatggccgt gttcaacggc gtcagcggcg cgaataccga cgacaccctg
     3601 cagaaattgc aggaaaccga agcgccgaaa ctggccgcgc accaggatgc gatctatctc
     3661 aacggcaagc tcttcgcccg cgtcgacgcg gtgtacaacg aacgcgcgaa attgaagctg
     3721 gatccggaat c
//
LOCUS       DASUCJ010000799         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_298787, whole genome shotgun sequence.
ACCESSION   DASUCJ010000799 DASUCJ010000000
VERSION     DASUCJ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298787"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="VFU90_09550"
     CDS             <1..>1051
                     /locus_tag="VFU90_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5480065.1"
                     /translation="EVPAPAAGVLAEILLDPDAEAVPGALLARIETKGIAVAPSATGK
                     VEARREQMTPRPASAPGNGTETRLSPSVRRALQQHGIDPSRIQGTGRDGRLTREDVDR
                     CVADATVVSVGERSTAQPMQFSAHDIPHDRMRMTIAENMARAVAEQPHVTAVFEADFS
                     AVAAHKAAMAAKGMKLSYTAYIVKAAAEAMAVAPAINGRWEKDRIAISPTIDVGIGTS
                     LGEKGLVVPVVKDAGALGLEQIGAKLDDLTRRARDGKLERSDVSGGSFTISNHGVSGS
                     LIAAPIILHQGQAAILGVGKLQKRVVVRTVNGRDAMLIHPMAYVTLTIDHRVVDGHQT
                     NAWLSRFVEIIEGWPA"
BASE COUNT          203 a          351 c          340 g          157 t
ORIGIN      
        1 ggaagtgccg gcgccggcgg ctggcgtgct cgccgagatc ctcctcgacc cggacgccga
       61 ggcggtgccc ggagcattgc tggcgcggat cgaaacgaaa gggatcgcgg tcgcaccaag
      121 tgccactggg aaggtcgaag cccggcgcga acaaatgact cctagacccg cgtctgcgcc
      181 ggggaacggc accgaaaccc gcctcagccc atcggtacgc cgcgccttgc agcagcacgg
      241 cattgatccg tcgcgcatcc aggggaccgg ccgcgacggg cgacttacgc gcgaagatgt
      301 cgaccgctgc gttgccgacg cgaccgtggt cagcgtcggc gaacgctcaa ctgcacagcc
      361 catgcaattt tccgcccacg acattcccca cgaccgcatg cggatgacaa tcgccgagaa
      421 catggcgcgc gcggtcgccg agcagccgca cgttactgcc gtgttcgaag ccgacttctc
      481 cgcggtggcg gcgcacaagg cagcgatggc cgccaaaggc atgaagctca gctacacggc
      541 atacatcgtc aaagccgcgg cggaagcgat ggcggttgct ccggccatca acggacgctg
      601 ggaaaaggac cggatcgcga tctcgccgac gatcgacgtc ggcatcggca cttcgctcgg
      661 cgagaagggt ttggtcgttc cggtcgtgaa ggacgccggc gcacttggcc tcgagcagat
      721 tggcgcgaag ctcgacgacc tcacccggcg tgcgcgcgac ggcaaactcg agcgatccga
      781 cgtgtcgggc ggaagcttca ccatttcgaa ccacggggtc tcgggctcgc tcatcgcggc
      841 cccgatcatc cttcatcagg gacaggccgc gattctgggc gttggcaaac ttcagaagcg
      901 cgtagtcgtc cgaaccgtga acggccgcga cgcgatgctc atccacccga tggcctatgt
      961 aaccctgacc atcgaccacc gtgtggtgga cggtcaccag acgaacgcct ggctcagccg
     1021 cttcgtcgaa attattgaag gctggccagc g
//
LOCUS       DASUCJ010000800         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_711593, whole genome shotgun sequence.
ACCESSION   DASUCJ010000800 DASUCJ010000000
VERSION     DASUCJ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711593"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFU90_09555"
     CDS             <1..280
                     /locus_tag="VFU90_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480066.1"
                     /translation="AELAREMIAAGVKARISCVDPSQIDRSFAGREFDAALLAELPAT
                     ADPCGEKGEFHTFVYDAPVFTRGIDVRSGVIVERDGFVFADVFSAPAI"
     gene            419..1345
                     /locus_tag="VFU90_09560"
     CDS             419..1345
                     /locus_tag="VFU90_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480067.1"
                     /translation="MRSRTIVVARATIAGVAVFALGACGGSSNTTGPKTGVPPAAVQQ
                     ELATTLATSIAAQIQAMTSTGASPFLGLFDRAPAMATLSTKARSRLHALAFTNCPEFA
                     PTPIVDTDGDGIPDSVSETYTTANCTDNSNGTLSLSGTFVIKEPDPTTPSLDYHAGID
                     NFLLHDAVTSGTEQVDITLGLGGTLDIAETIGSITEDGNYYISVNETKPQQVKDSVAA
                     NLTATYTFPADALLVEGQPLPGGTFSANGTESFTFDGTTYAFTLATATPLTVDPNCAT
                     SVTAGELTLTFGSAGSVHITWTGCGVYSVAQS"
     gene            complement(1370..>1724)
                     /locus_tag="VFU90_09565"
     CDS             complement(1370..>1724)
                     /locus_tag="VFU90_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480068.1"
                     /translation="SAFFAEMESLRAHHLRGAEPATLDVLLSEYRWLRTGDILSLAFC
                     NGWSEVQHDERGGSAHLLGDRLVLTPDPFGGAAVPLAVPGRELPNAPFASPAAAEHAY
                     AGAPATTLTGVAAGA"
BASE COUNT          293 a          559 c          584 g          288 t
ORIGIN      
        1 ggccgaactt gcgcgggaga tgattgccgc cggcgtgaaa gcgcgcatta gttgtgtgga
       61 cccatcgcag atcgaccgat cgttcgccgg tcgcgagttc gatgcggcgc tcctggccga
      121 gctgcctgcg acggccgatc cgtgcggcga gaagggcgaa ttccacacgt tcgtctacga
      181 cgcgcccgtg ttcacgcgcg gcatcgacgt ccggtcggga gtgattgtcg agcgagatgg
      241 atttgtgttt gccgacgtgt tcagcgcgcc ggcaatttga gttaggcatg gcctcgcgtg
      301 gcgggctctg ccgggcgatg agaagatgtt cgtcggcgcc acgtctctcg tagttggcag
      361 cgatgtcgct gccgcacacc gcgctgtctt cccatttttc gtgtcggagt gtcaaatcat
      421 gcgttcgcgt accatcgtgg tggcccgtgc gacgattgcc ggggttgcag tgtttgcgct
      481 cggtgcgtgc gggggaagta gcaatacgac aggtccgaaa accggtgtgc cgccggcagc
      541 cgttcagcaa gagttggcca ccacgctcgc gacgtcgatc gcggcgcaga tccaggcaat
      601 gacgtcgacg ggcgcatcgc cgttcctcgg attgttcgac cgggcgcccg cgatggccac
      661 cttgtcgacg aaggcgcgct cgcggctgca tgcgctggcg ttcacgaact gtcctgaatt
      721 tgcgccgaca ccgattgtcg acaccgacgg ggacgggatt ccggacagcg tgagcgagac
      781 gtacaccacc gccaactgca cggacaactc gaacggcacg ctgtcgctga gcgggacgtt
      841 cgtgatcaag gagccggacc cgaccacgcc ctcgctcgac taccacgccg gcatcgacaa
      901 cttcctgctg cacgatgcgg tgacgagcgg caccgagcag gtggacatca cgttaggcct
      961 cggcggcacg ctcgacatcg ccgagacgat cggttcgatc accgaggacg gcaactacta
     1021 catctcggtc aacgaaacga agccacaaca ggtgaaggac agcgtggccg cgaatctcac
     1081 ggcgacgtac accttcccgg cggatgcgct gctcgtcgag ggtcagccgc tgcccggcgg
     1141 tacgttctcg gccaacggca ccgagtcgtt cacgttcgac ggcacgacct acgcgttcac
     1201 gctcgcgacg gcgacgccgc tgacggtcga cccgaactgc gcgacgagtg tgacggccgg
     1261 cgagctcaca ctcaccttcg gcagcgccgg ttcggtgcac atcacgtgga cgggctgcgg
     1321 cgtgtactcg gtcgcgcaat cgtaagcgct ggacgcccgc atcgtgacgt taggcgccgg
     1381 cggcgactcc cgtgagagtc gtcgccggcg ctcctgcata tgcgtgctcg gcggccgcgg
     1441 gtgacgcgaa tggtgcgtta ggcagctcgc ggccggggac ggcgagcgga acggcggcgc
     1501 cgccgaacgg gtccggcgtc agcaccaacc ggtcgcccaa caggtgggcc gagccgccgc
     1561 gctcgtcgtg ctgcacctcg ctccagccgt tacaaaaggc gagcgagaga atgtctcccg
     1621 ttcgcagcca gcgatactcc gagagcagca cgtccagcgt ggccggctcg gcgccccgca
     1681 ggtggtgcgc gcgcagcgat tccatctccg cgaagaacgc cgac
//
LOCUS       DASUCJ010000801         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_360071, whole genome shotgun sequence.
ACCESSION   DASUCJ010000801 DASUCJ010000000
VERSION     DASUCJ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360071"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU90_09570"
     CDS             <1..723
                     /locus_tag="VFU90_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5480069.1"
                     /translation="ELLGAISHELRSPLGRARIALEIARDRVGDGAGLAPLDEVEKQL
                     SAVDTILGDLLDVTRAGLADLRLQTRPFVPWLRERVADEPTPPPVSVRMDAPAESPPL
                     AFDAALLARVVHNLLVNARAHGHPADVPIEVRVEAVDSQRAPTGGAERGSWIRVLVRD
                     RGAGFPPGFADRAFEPFVRGDASRSRPKEGAGYGLGLTIVRRIVEAHGGRVFARNAAD
                     AAGAEVGFELPCPPAERGAPDA"
     gene            791..1426
                     /gene="lepB"
                     /locus_tag="VFU90_09575"
     CDS             791..1426
                     /gene="lepB"
                     /locus_tag="VFU90_09575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5480070.1"
                     /translation="MKTVLSFVAWVAAIVSVTLFVVERVWLEAWKVPTDDPMLSASIE
                     PQLSAGDVVLVWRGPTFVRGNLLRCPDPQAAGRYVIARAIAAPGDNLSIDHEGISLDG
                     HAMRSPHACGQHDYTVFDPGRDESVNLTCITQEYGDRDFSALISADHPEPATKAHIDV
                     GQWFLVSDNRHIHVDSRDYGPIDASGCRHIVFRLWGRDGLGDANKRLSLVW"
     gene            1606..>1980
                     /locus_tag="VFU90_09580"
     CDS             1606..>1980
                     /locus_tag="VFU90_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5480071.1"
                     /translation="MPKSPVPKSPVPKSPVPSLWSPDDLKRRRQPSAPSLAGDAVPGD
                     AAVIEGADAGGVAVRAQAAETALSSANVSDDGIAPETHGRSELSQEELELMDQAKNYV
                     CKSCSTPVPLGHKFCGRCGAAIP"
BASE COUNT          291 a          681 c          681 g          327 t
ORIGIN      
        1 gagctcctcg gcgccatcag ccacgagctc cggtcccccc tcggccgggc gcgtatcgcc
       61 ctcgagatcg cgcgcgatcg cgtcggcgac ggggccggac tcgcgcccct cgacgaagtg
      121 gagaagcagc tctccgccgt cgatacgatt ctgggcgacc tgctcgacgt cactcgcgcc
      181 ggtcttgcgg acttgcgtct gcagacgcga ccgttcgtgc cgtggctccg cgaacgggtg
      241 gccgacgagc ccacaccgcc gcccgtttcc gttcggatgg acgcccccgc cgaaagcccc
      301 ccattggcct tcgacgccgc cctcctcgcg cgcgtcgtcc acaacttgct ggtcaacgcg
      361 cgcgcccacg ggcaccccgc cgacgtccct atcgaggtgc gcgtcgaggc cgtcgactcg
      421 caacgcgctc cgaccggcgg cgcggagcgt ggctcctgga ttcgggtcct cgtgcgtgat
      481 cgcggcgcgg gctttcctcc cgggttcgcc gatcgtgctt tcgagccgtt cgtgcgcggc
      541 gacgcgtccc gctcgcggcc gaaagaaggc gcgggctacg ggctggggct caccatcgtg
      601 cgccgcatcg tcgaggccca cggcgggcgc gtcttcgcgc ggaacgccgc ggatgcggcg
      661 ggggccgagg tgggcttcga attgccatgc ccgcctgccg agcggggcgc gcccgacgca
      721 tgaccctgag gtacgaacgg cggacgcacg actaacgtgc gtgccggtag gaaccgtaag
      781 aaagagccgg atgaagacgg tgcttagctt cgttgcctgg gtggcggcga tcgtctccgt
      841 gacgctgttc gtggtggagc gcgtatggct cgaggcgtgg aaagtcccga cggacgatcc
      901 catgctctcc gcgtcgatcg agccgcagct gagcgccggc gacgtcgtgc tcgtctggcg
      961 cggccctacc ttcgtgcgcg gcaacctcct gcgttgcccc gatccgcagg ccgcaggccg
     1021 ttacgtcatc gcgcgcgcca tcgccgcgcc cggcgacaat ctctcgatcg accatgaagg
     1081 catcagcctc gacgggcacg ccatgcggag tccacacgca tgcggacagc acgactacac
     1141 ggtgttcgat ccggggcggg atgagagcgt gaatctcacg tgcattacgc aggaatacgg
     1201 ggaccgggat ttttcggcgc tcatatcggc cgatcacccc gagccggcga cgaaggcgca
     1261 catcgacgtg ggccagtggt ttctcgtcag cgacaaccgc catatccatg tggactcacg
     1321 ggattacggc cccatcgatg cgtcgggctg ccgccacatc gtgtttcgcc tttggggccg
     1381 tgacggcttg ggagacgcga acaaacgcct gtctctcgta tggtgaccgc ggccggggta
     1441 agtttgccga gcttgcccct ttccgccgag aaggtatccg ccgaggtcgg ggtcgtttgc
     1501 ggcgtctgcg atcgttacag tgcgatcggc accgcagtct gctcgtgcgg ccgtccgctc
     1561 gtcatcgaac gcctcgtatc cgcgaagccc cctccggcga ggcgggtgcc gaagtcgccg
     1621 gtgccgaagt cgccggtgcc gaagtcgccg gtgcctagct tgtggagccc cgacgacttg
     1681 aagcgccgcc gtcagccgtc ggcccccagc cttgcgggcg acgcggtgcc cggcgacgcg
     1741 gccgttatcg agggggcgga cgccggcgga gtcgcagttc gtgcgcaagc agccgaaaca
     1801 gcgctatctt cggcgaacgt atccgatgat gggatcgcgc ccgaaacgca cggacgatcc
     1861 gagctgtcgc aggaggagct ggagctgatg gatcaggcca agaactacgt ttgcaaatct
     1921 tgctcgaccc cggtgccgct gggccacaag ttctgcggcc ggtgcggggc ggcgatcccg
//
LOCUS       DASUCJ010000802         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1170111, whole genome shotgun sequence.
ACCESSION   DASUCJ010000802 DASUCJ010000000
VERSION     DASUCJ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170111"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VFU90_09585"
     CDS             complement(<1..1184)
                     /locus_tag="VFU90_09585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5480072.1"
                     /translation="MQQIAPLLEMRGISKSFPGVQALSDVSLTLFAGEILVLVGENGA
                     GKSTLMKILSGAQRADRGDILIDGTPVQIATPRAAERLGIGMIYQEFTLVPQLTPPQN
                     IALGAEPLRAKLFVDDRAVRERAAAELADLGLELPLDVPTSELSVGQQQLVEIAKALA
                     RDARILVMDEPTAALSDREIERLFAIIGRLKAKGVGIIYISHRMDELPRIADRVTVLR
                     DGKAVETRRAADFPIGEIIRAMVGRQLDAHFPDLPPVAPDAPVVLAVEDLQREPGVRD
                     VSLRVRAGEIVGLAGLVGAGRTEMLRAIAGADVPRRGEIAVDGKPVRIRRPEDAIAAG
                     IAFITEDRKAQGLVLGMTVRENITLAHLAEFVARDRLIDGRREREAAAAMVDELHVRT
                     PG"
     gene            complement(1209..2174)
                     /locus_tag="VFU90_09590"
     CDS             complement(1209..2174)
                     /locus_tag="VFU90_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5480073.1"
                     /translation="MKAIRTLSILTICLAVAACSNSKTQATESSAGPSGGAASGAKTI
                     GVSIQNREAQFYQDMEAGMRGEAATYGYKLLVVDANRDNAKQQSQVEDFISKKVDAIV
                     LTPYDSQAIGSAIVEANNASIPVFTADIASTSNQGKVVAHIASDNVQGGYQAGKLICQ
                     AVGKSGDVAIVDEPEVTSVQDRVKGFKQALAQLCPGVTVVADVDSGGTRTKANSDTGD
                     ILQAHKDLKGIFGINDDSALGALTAVKAAGMTGKIAIVGYDATPEARAAIAAGTMYGD
                     AIQFPKKIGATTIDVIHDYFAGKTPAPVVKIGVGAFTQADAKGAH"
     gene            complement(2289..>2494)
                     /locus_tag="VFU90_09595"
     CDS             complement(2289..>2494)
                     /locus_tag="VFU90_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480074.1"
                     /translation="EASGLPPSPDSPAQSLLGWVLGLAGVYGALFSAGEFVYGRPAGG
                     TVWAVVAVASAAGLALLGRRLWE"
BASE COUNT          410 a          840 c          787 g          457 t
ORIGIN      
        1 atccccgggg tgcgaacgtg cagttcgtcg accatcgccg ccgccgcttc ccgctcgcgg
       61 cggccgtcga tcaaccgatc gcgcgccacg aattcggcca agtgcgcgag cgtgatattc
      121 tcgcgcaccg tcatcccgag caccaggccc tgagccttgc gatcctcggt gatgaacgcg
      181 atgcccgccg cgatggcgtc ttccggccgg cgaatgcgaa ccggctttcc atcgaccgca
      241 atctcgccgc ggcgaggaac gtcggcgccc gcaatcgccc gcagcatctc ggtgcggccg
      301 gcacctacca ggccggccaa gccgacgatc tcgccggcgc gcacccgcag gctcacgtcg
      361 cgcacccccg gctcgcgctg cagatcttcg acggcaagga cgaccggcgc gtccggcgcg
      421 accggcggca gatccgggaa gtgcgcgtcg agctgccgcc cgaccatcgc gcggatgatt
      481 tcgccgatcg gaaaatcggc cgcgcggcgc gtttccacgg ctttgccgtc gcgcagcacg
      541 gtcacgcgat cggcgatgcg cggcagctcg tccattcggt gcgagatgta gatgatgccg
      601 acgcccttgg ccttcagccg cccgatgatc gcgaaaaggc gctcgatctc gcgatcgctc
      661 agcgccgcgg tcggctcgtc catcaccagg atccgcgcgt cgcgggcgag cgctttggcg
      721 atctcgacga gctgctgctg cccgaccgaa agctccgagg tcggaacgtc gagcggtaac
      781 tccaagccga gatccgcaag ctccgcggcc gcgcgctcgc gaacggcgcg gtcgtcgacg
      841 aacagtttcg cccgcagcgg ttcggcaccc agcgcgatgt tttgcggcgg cgtcagctgc
      901 ggcaccagcg tgaattcctg gtagatcatc ccgattccga gccgctcggc ggctcgcggc
      961 gtcgcgatct gcaccggcgt tccgtcgatt aggatatcgc cgcggtcggc ccgctgcgcg
     1021 cccgaaagga ttttcatcag cgtcgatttt ccggcgccgt tttcacccac cagaaccaag
     1081 atttcgcccg caaacagcgt cagcgaaacg tcggaaaggg cctgcacccc cggaaacgac
     1141 ttggagatgc cgcgcatctc gagcagcgga gcgatttgct gcaacgtttt tgacttccgg
     1201 ccgtcccctt agtgcgcgcc cttcgcgtcg gcttgcgtga acgcgccgac gccgatcttc
     1261 accaccggcg cgggcgtctt cccggcgaaa tagtcgtgga tgacgtcgat cgtcgtagcg
     1321 ccgatcttct tcggaaattg aatggcgtcg ccgtacatcg ttccggcggc gatcgccgcg
     1381 cgggcctcgg gcgtggcgtc gtagccgacg atcgcaatct tgccggtcat cccggcagct
     1441 ttcaccgcgg tgagcgcgcc cagcgccgag tcgtcgttga taccgaagat acctttgaga
     1501 tctttgtgcg cttgcaggat gtcgccggtg tcgctgttcg ctttggtgcg ggtgcctccg
     1561 gaatccacgt cggcaacgac ggtcacgccg gggcacagct gcgcgagcgc ctgtttgaaa
     1621 cccttgacgc gatcttgaac gctggtgact tccggctcgt cgacgatcgc gacgtcgccg
     1681 cttttcccaa ccgcctggca gatcagcttc ccggcttgat agccgccctg cacgttatcg
     1741 ctcgcgatgt gggcgaccac tttgccttga ttgctggtgc tggcgatatc ggcggtaaag
     1801 accggaatcg atgcgttgtt ggcctcgacg attgcgctgc cgatcgcctg cgagtcgtac
     1861 ggcgtcaaaa cgattgcgtc gactttctta gagatgaagt cttcgacttg gctttgctgc
     1921 ttggcattat cgcgattggc gtcgacgacg agcagcttgt agccgtacgt tgcggcctcg
     1981 ccgcgcatgc cggcttccat atcctggtag aactgcgctt cgcgattctg gatggaaacg
     2041 ccgatggttt tcgcgcccga ggccgctccg ccggagggtc cagctgagga ctcggtcgct
     2101 tgcgtcttcg aattcgagca tgcggcaacc gccaggcaaa tcgtaagaat cgataaggtc
     2161 cggattgctt tcaagtcgtt ctcctaagac ggccgggtgc caagccagtg gttgggcaga
     2221 agtcgaacgc tagccttcgt tgtccacaaa ctgcgacgtt cgcttgtgca atctgtggag
     2281 aatgcacgtt attcccacag gcgacggccc aaaagggcaa gtccggcggc gctcgcgacc
     2341 gcgacgacgg cccaaacggt gccgccggcg gggcgcccgt aaacgaactc tccggccgaa
     2401 aaaagggcgc cgtagacgcc cgccagcccc aagacccacc cgagcaacga ctgcgccggc
     2461 gagtccggcg agggcggcag acccgacgcc tcgc
//
LOCUS       DASUCJ010000803         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_604420, whole genome shotgun sequence.
ACCESSION   DASUCJ010000803 DASUCJ010000000
VERSION     DASUCJ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604420"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFU90_09600"
     CDS             complement(<1..548)
                     /locus_tag="VFU90_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480075.1"
                     /translation="MMSSSRLRDAGIAACAAVALAAAFPKLGAAWIVPFGTAALFWAW
                     QGASWKSAAAIGWFSGMIFFAIGFDWIGHTVASYIGPYGPFLMLGPALVEAPYFALAA
                     VLAAVAYRYARPEFAPLAAAAAFAFAEWLRSVGLLGVPFDQLGYTQAGTPLRAIAAYA
                     GTYGVTFVLCALGAYLADAIRRR"
     gene            547..1284
                     /locus_tag="VFU90_09605"
     CDS             547..1284
                     /locus_tag="VFU90_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480076.1"
                     /translation="MRRLSFGFVVVTAFIVAACGRQVTPNPPGLGAGGAAPGYMSVTF
                     DVNAPFNFSNYQYWVVFNTTGNGLTPDTHPFQSNWAAYSSALEVAGVGGATFARAIQF
                     VKNSNPHIPPQFQPLITTPQQFQYNANSNGSGTEFSIIFQRSIFTSIASPGPGPTPTP
                     FSNNWRFNAFTTQANPSNQLIYVDSMGAGGPVDPQWNSPNIPITVCADQTYYALSSGL
                     QIDPPALIESVDIGNNPSPAPGASPCS"
     gene            complement(1295..>3286)
                     /locus_tag="VFU90_09610"
     CDS             complement(1295..>3286)
                     /locus_tag="VFU90_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480077.1"
                     /translation="YVAVIADRVAGGTHKGQPGDAIGNVHIYYGDEEIVGDKAHFDGI
                     RTITITGNPFLINHTHDSVLAGDVIFFDTIEETAKLVNGNGETAQGIERGFVHFRAGD
                     LHTDPDGTAHGLNPSVTTCENPRAGYHITGKSIDVVPGDKIVISKAILWLGAAAVFYL
                     PHLVIPLRTVQDQRARPHWFPEVGYDSYEGAWIKIQVPFGKDQYYYGYYIVNYFTKEG
                     LGLGYVGFYASKKGRRTVSINFYGMNNHLAGGKQYNIAAQEQENFSQHLRGNFQFGYQ
                     SNYGALTNVPPNENLSGAIVHQTQNTSQNYSYNHSSVGTQSSSNSFSFTDTRQFNQQL
                     SQTVSFNVSNSASNFAGESSFNQTSEFDYLMHYTTAGADYQLEYDKTFAQQPFGIDKI
                     PEFQVRPYDFFQHFFIPMSAQLTVGEYSEPTSQLATWRGDAGLVLGPALAKIWGSDFE
                     ATVNVDQYAYGTGDLKAAVQQDLSLTTPIGSHVVNALTYNEANYNGPALVPFQYLDQQ
                     PTENTKNAQDLIRFFNDDVYSVSLGFSTNFDGIAQPVSYQITARPSARSVVLLGGSFI
                     PGPGLGFETTNLQLSTPFGRDASLQFVTDLQWKGPEFFSNKIIYYTRTIGNCYQLQVL
                     YSEASQSVNVGLSLLAFPNQTATFAIGQSGSIIPSTFNF"
BASE COUNT          616 a          999 c         1038 g          633 t
ORIGIN      
        1 cgccggcgaa ttgcgtcggc gagatacgca ccgagcgcgc acagcacgaa cgtgacgccg
       61 tacgtccccg cgtacgccgc gatcgcgcgc agcggcgtcc cggcttgcgt gtatccgagt
      121 tgatcgaacg gtacgccgag caaaccgacg gaacgcagcc attcggcaaa cgcgaaagcc
      181 gccgccgccg ccagcggcgc gaactccggc cgcgcgtaac ggtaggcgac ggccgcgagc
      241 accgcggcga gcgcgaagta cggcgcctcc accagcgccg gtccgagcat cagaaacgga
      301 ccgtagggtc cgatgtagct ggcgaccgtg tgcccgatcc aatcgaatcc gattgcaaag
      361 aagatcatgc cggaaaacca gccgatcgcc gccgccgatt tccaggaagc tccttgccac
      421 gcccagaaca gcgcggcggt tccaaaagga acgatccagg cggcgccgag cttggggaaa
      481 gccgcggcga gcgcgaccgc cgcacacgcg gcgatcccgg cgtcgcgcag ccgtgacgag
      541 gacatcatgc gcaggttgag tttcggtttc gtggttgtta cggcgttcat cgttgcggcg
      601 tgcggccgac aggtcacgcc gaaccctccc ggtttgggag cgggcggcgc ggctccgggc
      661 tacatgagcg tgacgtttga cgtcaatgct ccattcaact tttcaaacta ccaatattgg
      721 gtagtgttca atacgacggg caacggactg acgcccgata cccatccgtt ccagagcaac
      781 tgggcggcat attcgtcggc gctcgaggtg gccggcgtcg gcggcgcgac cttcgcgcgc
      841 gccatccagt tcgtcaagaa ctcgaacccg catattccgc ctcagttcca gccgctgatc
      901 acgacgccgc agcagttcca gtacaacgcc aacagcaacg gctccgggac cgagttctcg
      961 atcatcttcc agcgctcgat tttcacgagc atcgcctcgc ccggaccagg tcccacgcct
     1021 acgccgtttt cgaacaactg gcgtttcaac gcgttcacca cccaagccaa cccctccaat
     1081 cagctgatct acgtcgactc gatgggcgcc ggcggaccgg tggacccgca gtggaattcc
     1141 ccgaacatcc cgataacggt atgcgccgac caaacgtact acgcgctctc gagcggcctg
     1201 cagatcgacc cgcccgcgct gatcgaaagc gtcgacatcg gcaacaatcc cagccccgcg
     1261 ccgggagcgt ctccctgctc gtaggcggcc gttcctagaa gttgaacgtc gacgggatga
     1321 tcgagccgct ctggccgatc gcgaacgtcg ccgtctgatt cggaaaggcg agcaggctca
     1381 agccgacgtt gaccgactgc gaggcttcgc tgtagagcac ttgcagttgg tagcagttgc
     1441 cgatcgtccg cgtatagtag atgatcttgt tggagaaaaa ctccggccct ttccactgta
     1501 aatcggtaac gaactgcagc gacgcatcgc gcccgaacgg cgtagagagc tgcaagttcg
     1561 tcgtttcgaa tcccaaaccg ggcccgggaa tgaacgaacc gcccagcagc accaccgatc
     1621 gcgccgacgg gcgcgcggtg atttgatagc tgaccggctg cgcgatcccg tcgaagttcg
     1681 tcgagaagcc gagcgatacc gaatacacgt cgtcgttgaa gaagcggatg agatcctggg
     1741 cgttcttcgt attctcggtc ggctgttgat cgagatactg gaacggcacg agcgccggac
     1801 cgttgtagtt ggcttcgttg tacgtcagcg cgttgacgac gtgcgacccg atcggcgtcg
     1861 tgaggctgag atcttgctgc acggcggcct tgaggtcgcc ggttccgtag gcgtactgat
     1921 cgacgttgac ggtcgcctcg aagtcgcttc cccaaatctt cgcgagcgcc ggtccgagca
     1981 ccaagcccgc gtcgccccgc caggtcgcga gctggctggt cggttcgctg tattcgccta
     2041 ccgtgagctg ggccgacatc gggatgaaga aatgctggaa gaagtcgtac ggccgcactt
     2101 gaaactcggg aatcttgtcg atcccgaagg gctgctgcgc gaacgtcttg tcgtactcca
     2161 gctgatagtc cgcgcccgcc gtggtgtagt gcatcaggta gtcgaactcg gaggtctgat
     2221 tgaacgacga ttcgcccgcg aaattcgagg cgctgttgga aacgttgaag ctgacggttt
     2281 ggctcagctg ctggttgaac tgccgcgtat cggtgaacga aaagctgttg ctgctggact
     2341 gggttcccac cgagctgtgg ttgtacgaat agttctgcga cgtgttctgc gtctggtgga
     2401 cgatcgcgcc gctcaggttt tcgttgggcg gaacgttggt tagggcgcca tagttggact
     2461 gatagccgaa ctggaagttg ccgcgcaaat gctgcgagaa attttcctgc tcctgcgccg
     2521 cgatgttgta ctgcttcccg ccggcgaggt ggttgttcat cccgtagaaa ttgatgctga
     2581 cggtgcgccg gcctttcttg ctcgcgtaga agccgacgta cccgagtccg agcccttcct
     2641 tggtaaaata gttgacgatg taatagccgt agtaatattg gtctttcccg aacggcactt
     2701 gaatcttgat ccaggcgcct tcgtacgagt cgtagcccac ctccggaaac caatgcgggc
     2761 gcgcgcgctg gtcctgaacg gttcgcagcg gaatgaccag gtgcggcaag tagaacacgg
     2821 cggccgcccc gagccagagg atggccttgc tgatgacgat cttgtctccg gggacgacgt
     2881 cgatgctctt gccggtgatg tgatagccgg cgcgcggatt ctcgcaggtc gtcacgctcg
     2941 gattgagacc gtgggcggta ccgtcggggt cggtgtgcag atcgcccgcg cgaaagtgga
     3001 cgaagccgcg ctcgatgcct tgagcggttt cgccgttgcc gttgacgagc ttcgcggttt
     3061 cctcgatcgt atcgaaaaag atgacgtcgc ccgccagcac ggagtcgtgg gtgtggttga
     3121 tgagaaacgg attgccggtg atcgtgatcg tgcggatgcc gtcgaagtgg gctttgtcgc
     3181 cgacgatctc ttcgtcgccg tagtaaatgt gcacgttgcc gatcgcgtcg ccgggctggc
     3241 ccttgtgcgt gccgccggcc acgcggtcgg cgatgaccgc gacgta
//
LOCUS       DASUCJ010000804         1471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1384204, whole genome shotgun sequence.
ACCESSION   DASUCJ010000804 DASUCJ010000000
VERSION     DASUCJ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384204"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1471)
                     /locus_tag="VFU90_09615"
     CDS             complement(<1..>1471)
                     /locus_tag="VFU90_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480078.1"
                     /translation="SPFAIGLAIRATMTRDRTWPAYALGSSLLMAVIVASAQVFVFTL
                     GVIWAICLAMRWSWYSVLRLAALSGAAFALCAFWILPAFVAGGAVHTVVQTSPIETAF
                     ATLAQFSNPLMTLTMLAFPGDFYVHALGWGAPLYFLAYAGLIALCVTALIRHRTALLI
                     TLAAIFLLTALLPLGGNPVLGPVILAVFKAFLPYSLFLRTPQHLMFVMALVVPMLVYL
                     SARTIPNRLRKPSLAIAVAVVALYGQGFFLHGTFFGLIGPFRESAGERATVAAADFPG
                     NEEYRTMFVPNSPSYYYHPAIFDYYFEGSDEPQIRFLPGMSMGAGAKWTPYDRTQQLL
                     KALDELVPDGGDPQTQTMLLQMTGVKHIVVHQIGTPTAGIRLTAQTGRTYLDGALRRS
                     GIASLEADFGDRSLWRFNRPVPREYAPDCIFGVSPYADPYDVLALAPAAAGCRRPAAV
                     ASAASARSEEVFSAATFQTDRQPGVALARPASNVEIVTAN"
BASE COUNT          305 a          479 c          482 g          205 t
ORIGIN      
        1 cgttcgcggt aacgatttcg acgttcgacg ccgggcgcgc cagcgcgacg ccgggctgac
       61 ggtcggtttg aaaggttgcc gccgagaaca cttcttcgga acgggccgag gccgccgacg
      121 cgaccgccgc cggccgccgg cagcccgccg ccgccggtgc gagcgccagc acgtcgtacg
      181 gatcggcgta gggagatacg ccgaaaatgc agtcgggcgc gtactcgcgc ggcaccgggc
      241 ggttgaaacg ccacagcgac cggtcgccga aatcggcttc gagcgacgcg atcccgctgc
      301 ggcgcagcgc gccgtcgaga taggtccgtc cggtttgcgc ggtcaaccgg atgcccgccg
      361 tcggcgttcc gatttggtgg acgacgatgt gcttgacgcc ggtcatctgc agcagcatcg
      421 tctgcgtttg cgggtcgccg ccgtcgggaa cgagctcgtc gagcgccttg agcagctgct
      481 gcgtgcggtc gtacggcgtc cacttggctc cggcgcccat cgacatgccc ggcaaaaaac
      541 gaatttgcgg ctcgtccgac ccttcgaagt agtagtcgaa gatcgccgga tggtagtagt
      601 agctcggcga attcggcacg aacatcgtgc gatattcctc gttgccggga aagtcggcgg
      661 ccgccaccgt cgcgcgctct ccggccgatt cgcgaaacgg tccgatcagc ccgaagaagg
      721 taccgtgcag aaagaaccct tgaccgtaga gggccacgac cgccacggca atcgccagcg
      781 acggcttgcg caagcgattg gggatcgtgc gcgccgacag atagaccagc atcggaacga
      841 ccagtgccat cacgaacatc aggtgctgcg gcgtgcgcaa aaacaaactg tacggcaaga
      901 aggctttgaa gacggcgaga atgaccggtc cgagcaccgg gtttccaccc agcggcagca
      961 gcgccgtgag aagaaagatc gccgcgagcg tgatcagcag cgcggtccgg tgccggatca
     1021 gcgcggttac gcacagcgcg atgagtcccg cgtacgccag aaagtacagc ggtgcgcccc
     1081 agcccaacgc gtgcacgtaa aagtcgccgg gaaaggcgag catcgtcagc gtcatcagcg
     1141 ggttcgagaa ctgcgcgagc gtcgcgaacg ccgtctcgat cggcgacgtc tgcacgaccg
     1201 tatgcaccgc acccccggcg acgaaggccg gtaagatcca aaacgcgcac aaggcgaaag
     1261 cggctccgct cagcgccgcc agccgaagca ccgagtacca cgaccaacgc attgccaagc
     1321 aaatcgccca aatcacgccc agcgtaaaaa cgaacacttg cgcggaagcg acgatgacgg
     1381 ccatcaacag cgacgatccg agtgcgtaag ccggccacgt ccggtcgcgc gtcatcgtgg
     1441 cgcggatcgc gagtccaatc gcaaacgggc t
//
LOCUS       DASUCJ010000805         1208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_910356, whole genome shotgun sequence.
ACCESSION   DASUCJ010000805 DASUCJ010000000
VERSION     DASUCJ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910356"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1208)
                     /locus_tag="VFU90_09620"
     CDS             complement(<1..>1208)
                     /locus_tag="VFU90_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5480079.1"
                     /translation="LATVASVDSGAVGYVTALSNAWFKTHFDPSNHWTIFAVAGGIIV
                     LSAILNSVGAKIMGRVARYGVYVETIGTFGVFAALAFFGFHQHLGFIFSTQGVQHPAT
                     NPLHLDFGGHWWTGAALVAILANVYIFYGFESAGDISEETIDAQRQVPRAMRNALFYG
                     GIASFVLVLGLLLATPSAGIAGVVSSGINVVLAQLPTWLQDFFLVMVIVAFFSCGTAV
                     QGAGARVAFALARDGALPLSNALRKVSPRHQTPVNAILVGTIVPFLFLLLVLVNPSKP
                     VHILWFDYPANVNALYALVSFATSGIYLAFFLTVFGALLARLRGWVPSGVFTLGKWGI
                     PVTVGGALYLLLMLLNIVWPSALSSGRAAFNYGWVTLLVMAIIVAAGAIYEAIARPDR
                     NVAKHRTDTV"
BASE COUNT          245 a          343 c          447 g          173 t
ORIGIN      
        1 cacggtgtcg gtgcggtgtt ttgcgacgtt gcggtcggga cgggcgatgg cttcgtagat
       61 tgcgccggcg gcgacgatga tcgccatcac gagtagcgtt acccagccgt agttgaaggc
      121 ggcgcggccg ctggagagcg cgctcggcca gacgatgttc agcagcatca gcaagagata
      181 gagcgcgccg ccgacggtga cggggatgcc ccatttgccg agcgtgaaga cgccgctcgg
      241 gacccagccg cgcagacggg caagcagcgc gccgaacacg gtgaggaaga acgcgaggta
      301 gatgccggac gttgcgaagg aaacgagcgc gtagagcgcg ttgacgttgg ccgggtagtc
      361 gaaccacaga atgtgcacag gtttgctcgg gttgacgagc accaggagca gaaataagaa
      421 cggcacgatc gtaccgacca ggatcgcgtt gaccggcgtt tggtggcgcg gcgaaacttt
      481 gcgtaaggcg ttgctcaacg gcagcgcgcc gtcgcgggcg agcgcgaagg cgacgcgggc
      541 gccggcgcct tgcacggccg tgccgcagct gaagaacgcg acgatcacca tcacgaggaa
      601 gaaatcttgc agccacgtgg gcagctgcgc gagcacgacg ttgatgccgc tgctcacgac
      661 gccggcgatc ccggcgctgg gcgtggcgag cagcaggccg aggaccagca cgaacgaggc
      721 gatcccgccg tagaacagcg cgttgcgcat cgcgcgcggg acttggcgct gcgcgtcgat
      781 ggtttcttcg gagatatcgc cggccgattc gaagccgtag aagatgtaga cgttggcgag
      841 gatcgcgacg agcgcggcgc cggtccacca gtggccgccg aagtctaagt gcagcgggtt
      901 ggtcgccgga tgctgcacgc cttgggtgct gaagatgaag ccgaggtgct ggtggaagcc
      961 gaagaacgcg agcgccgcaa aaacgccgaa cgtgccgatc gtttcgacgt agacgccgta
     1021 gcgggcgacg cggcccatga tcttcgcgcc gaccgaattc aaaatggcgg aaagcacgat
     1081 gatgccgccg gcgacggcga agatcgtcca gtggttggag gggtcgaaat gcgttttgaa
     1141 ccaggcgttc gagagcgcgg tgacgtagcc gaccgcgccc gaatcgaccg acgcgacggt
     1201 cgcgagca
//
LOCUS       DASUCJ010000806         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1200792, whole genome shotgun sequence.
ACCESSION   DASUCJ010000806 DASUCJ010000000
VERSION     DASUCJ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200792"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VFU90_09625"
     CDS             complement(<1..1371)
                     /locus_tag="VFU90_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5480080.1"
                     /translation="MIAAAAAVLAALSAIGFPVTTSDPEAQALIDRGLFLYYAYDGGD
                     AAGAFAAAGRRDPRLAMAPWGEALADGPDLNTPMTAERFARAQRAAQAAIALEAGASP
                     SERAYLDATALRYNGTWDDWPSDDAAYRTAMTAVAQRSSGPGGNDAKVLAAEALLEHG
                     GLAWDAAQPAAGDSRRALELVDAVLASDPNNVMANHLCIHLYDDAADRTPALPCARRL
                     DAAAFPPQAEHLAHMPAHYWIETGNYAAALASSERAYRLFTELQRVPDRNPEHDRYLQ
                     HDVYVGYSAALMLGNYATAQEWSARASQVFATPYDSLTALRFGRFDEAYRLAADASPA
                     GLAVRGYAALQLGRLSEARALGDRLRRITTAGYLAQLFFARLAETDGRDDEAAAWIDR
                     AAEQQSATLSAELIPLLPALEARAGLALRQRRYDAAVAAYTQALDAFPNDPRASFGLA
                     QALQAAG"
     gene            complement(1368..>1836)
                     /locus_tag="VFU90_09630"
     CDS             complement(1368..>1836)
                     /locus_tag="VFU90_09630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5480081.1"
                     /translation="PDSPGKSRMSASMLDGFPLVSRFSVPFADIDMMQHVNNVAYIRW
                     AEMMRSEYFAQVMNEPINGAHGIIQATINFTYERQLHYRECIAVGCRISRMGNKSWDF
                     AYEIWSETHGHRAAYGITTVVAYDFIAQATIQIPQSWRDAIAAYEAGPQHFFV"
BASE COUNT          289 a          594 c          654 g          299 t
ORIGIN      
        1 gccggcagcc tgcagcgcct gcgcgagccc gaacgacgcg cgcggatcgt tgggaaacgc
       61 gtccagcgcc tgggtgtagg cggcaacggc ggcgtcgtaa cgccgttgcc gcaacgccag
      121 cccggcgcgc gcttcgagcg ctggcaacag cggaatcaac tcggccgaaa gcgttgcgga
      181 ctgctgctcg gccgcgcggt cgatccaagc ggcggcttcg tcgtcgcgac catcggtttc
      241 ggccaaccgc gcgaagaaca gctgcgcgag atagccggcc gtcgtaattc tccgcaaccg
      301 gtcgccgagc gctcgcgctt cggaaaggcg cccgagttgg agcgcggcgt agccccgcac
      361 cgcgaggccg gcgggagaag cgtcggcagc aagccgatac gcctcgtcga agcggccgaa
      421 gcgaagcgcc gtcagcgaat cgtacggggt cgcaaagacc tggctcgccc gagccgacca
      481 ctcctgcgcg gtggcgtagt ttccgagcat gagggccgcc gaatatccga cgtagacgtc
      541 gtgctgcagg taccggtcgt gttcggggtt gcgatcgggg acgcgctgca gttcggtgaa
      601 gagcctatag gcccgctcgc tggaagcgag cgcggccgca tagtttccgg tttcgatcca
      661 gtaatgcgcc ggcatgtgcg cgaggtgttc ggcctgcggc gggaaggcgg cggcgtcgag
      721 ccggcgcgcg cacggcaacg ccggcgtgcg atccgcggcg tcgtcgtaga gatgaatgca
      781 cagatggttg gccattacgt tattcgggtc gcttgcgagc acggcgtcga cgagttccag
      841 cgcgcggcgc gaatcgccgg ccgccggctg tgctgcgtcc caagcgagac cgccgtgttc
      901 gagcagcgct tcggccgcca gaaccttggc gtcgttgccg cccggcccgc tcgagcgctg
      961 cgcgacggcc gtcatcgccg tgcgataagc cgcgtcgtcg ctcggccagt cgtcccacgt
     1021 tccgttatat cgcagcgccg tcgcatcgag gtacgcgcgt tcgcttgggc tcgcgccggc
     1081 ctcgagggcg atggctgcct gcgcggcgcg ctgcgcgcgt gcgaaccgct cggcggtcat
     1141 cggcgtgttc aagtcgggac cgtcggccag cgcctccccc caaggcgcca ttgcgaggcg
     1201 cgggtctcgc cggccggcgg cggcgaacgc gccggcggcg tctccgccgt cgtaggcgta
     1261 gtagaggaag agcccgcgat cgatcagggc ctgcgcctcg gggtcggagg tcgtgaccgg
     1321 aaaaccaatc gcgctgagag cggcgaggac ggcagcggcg gcggctatca cacgaaaaag
     1381 tgctgcggac cggcctcgta cgcggcgatt gcgtcacgcc aggattgcgg tatttgaatc
     1441 gtcgcctggg cgatgaaatc gtacgccacg acggtcgtga ttccgtaggc ggcgcggtgc
     1501 ccgtgcgttt cgctccaaat ctcgtaggcg aagtcccacg acttgttccc catccgcgaa
     1561 atgcggcaac cgacggcaat gcattcccgg tagtggagct ggcgttcgta cgtgaagttg
     1621 atcgtcgcct ggatgatgcc gtgcgccccg ttgatcggtt cgttcatcac ctgcgcgaag
     1681 tattccgagc gcatcatctc ggcccagcgg atgtaggcga cgttgttaac gtgctgcatc
     1741 atgtcgatgt cggcgaacgg cacgctgaaa cgcgatacga gcgggaaccc gtcgagcatg
     1801 gacgcgctca ttcgactctt acccggcgag tccggg
//
LOCUS       DASUCJ010000807         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1047985, whole genome shotgun sequence.
ACCESSION   DASUCJ010000807 DASUCJ010000000
VERSION     DASUCJ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047985"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFU90_09635"
     CDS             <1..429
                     /locus_tag="VFU90_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480082.1"
                     /translation="DGKVHADGGIAFDHYESDETVTLRYSQNDGRAQSGLMVTDRADV
                     PILSVIEKQDSLRKMPAGAARDSATKAFVENGGHPLAARRLFAGRDADQSSLVSLNDP
                     HGRPRLRLMVDSTGAASIQFLDTLGRVKRTITADSLGGSR"
     gene            426..>1029
                     /locus_tag="VFU90_09640"
     CDS             426..>1029
                     /locus_tag="VFU90_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480083.1"
                     /translation="MNTRTWERRACLTAAALMVAGASAFAQSARFDVTVAPSAHDGPL
                     TGRLIVAVSKLDKPEPRLMISPRGPAIFGVDLDQLPAGHTAVVDNSASSYPMKLSALP
                     PGDYYVEAVVNVYQQFHRSDGHTIWVHMGDGRVEFFNFAGGNLYSDVQKVHIDGHSTV
                     HLTVTHVIPVEPRPADTEWLKHVKIQSQLLTTFWGHPVYIY"
BASE COUNT          188 a          344 c          330 g          167 t
ORIGIN      
        1 gacggtaagg tgcacgccga cggcggcatt gcattcgacc actacgagtc cgacgaaacc
       61 gtcacgctgc gctactcgca aaacgatgga cgcgcgcaat ccggactcat ggtcacggat
      121 cgtgcggacg tgccgattct cagcgtcatc gagaagcagg attcgcttcg caagatgccc
      181 gccggcgcgg cacgcgactc ggccacgaag gccttcgtgg agaacggcgg tcatccgtta
      241 gccgcgcggc ggttgttcgc cgggcgcgac gccgaccaga gttcgttggt cagcctgaac
      301 gatccacacg gtcgtccgcg gttgcgcctc atggtcgact cgacgggcgc ggccagcatc
      361 cagttcctcg acaccctggg ccgcgtgaaa cgaaccatca ccgccgattc gctcggagga
      421 tcgcgatgaa cacccgcacg tgggaaaggc gcgcttgcct aacggcagcc gcgctcatgg
      481 tcgccggcgc cagtgccttc gcgcagtcgg cgcggttcga cgtgacggtc gcgccatcgg
      541 cgcacgacgg tccgctcacc ggccgcctga tcgtcgccgt gagcaagctg gacaagcccg
      601 agccgaggct catgatctcg ccgcgcgggc cggcgatttt cggagtggat ctcgaccagc
      661 tgccggccgg ccatacggcc gtcgtcgaca attccgcatc gagctatccg atgaagctct
      721 cggcgctgcc gcccggcgac tactacgtgg aggcggtggt gaacgtctat cagcagtttc
      781 atcgcagcga tggccacacg atctgggtgc acatgggcga cggccgcgtg gagttcttca
      841 acttcgccgg cggcaacttg tacagcgacg tccagaaagt gcacatcgac gggcacagca
      901 cggtgcacct caccgtgacc cacgtgattc ccgtcgagcc gcggcccgcg gacacggagt
      961 ggctcaagca cgtaaagatc cagagccagc tgctcacgac gttctgggga cacccggtgt
     1021 acatctatg
//
LOCUS       DASUCJ010000808         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_54652, whole genome shotgun sequence.
ACCESSION   DASUCJ010000808 DASUCJ010000000
VERSION     DASUCJ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54652"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFU90_09645"
     CDS             <1..432
                     /locus_tag="VFU90_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480084.1"
                     /translation="DAAMEYQKQGTPLVVIAGKEYGTGSSRDWAAKGTILLGVRAVIA
                     ESFERIHRSNLVGMGVLPLELTNGDTRQSLGLTGFETFDIVGLDESLRPRAKLTVRAQ
                     LPNGGSKEFGVVARIDTPEEMQYYRHGGILPYVLRQLVGNR"
     gene            429..>1309
                     /locus_tag="VFU90_09650"
     CDS             429..>1309
                     /locus_tag="VFU90_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480085.1"
                     /translation="MTERQKTRLVVGALCLTAVANGCFLRKYVYRPVLVADAIGDSTT
                     MLSDTRLHVYRTDQYEVYGPTATSVSFASQQIDRAYREFARHFGVDAPYMAVVVADSA
                     FAISPSDAGSFAKRRIHTFVYVRPHNLRDIPGISPELSEDEIWPVSARVAREMLTAYI
                     TKRRHLAPEVETATHASDYHNDPLPVWYVDAVVALLSDPGAPDRVMDYLKDHLAEAPP
                     LSELLDAKPPLAGTPDTLGASPERRTIVGATGVGLMLYLVEHEGPRIVGRVADAFLAG
                     GAARDVVRQAKHVPQNDR"
BASE COUNT          225 a          428 c          445 g          211 t
ORIGIN      
        1 gacgccgcca tggaatatca gaagcaaggc accccgctcg tcgtgatcgc cggaaaggag
       61 tatggaacgg ggtcgtcgcg cgactgggcg gcgaagggca cgatcctcct cggcgtacgg
      121 gcggtgatcg cggaatcgtt cgagcgcatt catcgatcga acctcgtcgg catgggcgtg
      181 ctcccactgg agctcacgaa cggggacacg cggcagtcgt tgggtctcac gggcttcgag
      241 acgttcgaca tcgttggcct cgatgaatcc cttcgcccgc gcgcgaagct caccgtccgg
      301 gcacagctgc cgaacggggg cagcaaggag ttcggggtgg tagcgcggat agatacgccg
      361 gaggagatgc agtactaccg gcacggtggc atcctgccgt acgtgctgcg gcagctcgtc
      421 ggaaaccgat gacggagcgg cagaagacgc ggctcgtggt cggcgcgctg tgcctaacgg
      481 ctgtggccaa cgggtgcttt ttgcggaagt acgtctatcg gcccgtgctg gtcgccgacg
      541 ccatcggcga ctcgaccacc atgctgtcgg acactcggct gcacgtctat cgcacggacc
      601 agtacgaggt gtacggccca acggcgacgt cggtgagctt cgcgtcgcag cagatcgacc
      661 gtgcctatcg tgagttcgcc aggcacttcg gcgtcgacgc gccgtacatg gcggtggtcg
      721 tcgccgacag cgccttcgcg atctcgccaa gcgacgcggg cagcttcgcg aagcgccgca
      781 tccacacatt cgtgtacgtg cggccgcaca acctgcggga catacccggc atcagtcccg
      841 agttgtccga ggacgaaatc tggccggtgt cggcgcgcgt cgcgcgcgag atgctcaccg
      901 cgtacatcac gaagcgacga caccttgccc ccgaagtcga aacggccacg cacgcgagcg
      961 actatcacaa cgatccgctc ccggtctggt acgtcgatgc agtcgtcgcg ctgctcagcg
     1021 atccgggcgc tccggatcgc gtcatggact acctgaagga tcacctcgcg gaggcgccgc
     1081 cgctctccga gctgctcgac gccaagccgc cgttggcggg cacccccgac acgctcggcg
     1141 cgtcaccgga gcggcgaacg atcgtcggcg cgactggcgt tggattgatg ctgtatctcg
     1201 tcgagcacga gggaccgcgg atcgtgggac gcgttgcgga cgcctttctc gccggcggtg
     1261 cggcgcgcga tgtcgtccgg caggccaagc acgtcccgca gaacgaccg
//
LOCUS       DASUCJ010000809         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_558946, whole genome shotgun sequence.
ACCESSION   DASUCJ010000809 DASUCJ010000000
VERSION     DASUCJ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558946"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..774)
                     /locus_tag="VFU90_09655"
     CDS             complement(112..774)
                     /locus_tag="VFU90_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480086.1"
                     /translation="MLLPGAHALRVPNLVDSSADVAVFTQRPTGDTTTRLVAHVAMAA
                     LRSDDGIRLTTNWTPPFQSSDTLIVDARSLRPVSEVLAFNHVRYAYRYAGAHVTGTIQ
                     HPDSAPRVFDQTFDVPVFAFNEVKPLVRSLEYRSGAQMVVPLFSEVDGALERDTLTVL
                     GRTRETGVDAWVVRFADPVITTRYLVDAHSRAILDAVTTQKKSGFRFHYAYATAKPRR
                     AG"
     gene            complement(910..>1533)
                     /locus_tag="VFU90_09660"
     CDS             complement(910..>1533)
                     /locus_tag="VFU90_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480087.1"
                     /translation="YAGRVRVAKITAGRVTPLVDATFTTVFRDSAGVPLMFMSSNSGG
                     ATGVTVLDRRTLAPYAIIQQCGTMRFQGTSIDGSQCQSGGASTPIHVKLETPAFFGNA
                     ADLVVEVLPRRTNVVYRMVLWQGNDSTETHLYRTTGREHVRVGGTLYRRAWVVDDYVA
                     GSTGGSNRLASRMWIIDAPPYMVRWVFYNTPAPGSEVRAEQSVTHLP"
BASE COUNT          271 a          492 c          521 g          249 t
ORIGIN      
        1 atggctgccg acaggcgctc ggccagcgac cgctgccgcc ggtggcctcg cgccgtcgcg
       61 catacgcgcc gacgatgcag acggcggtcg caaagcgcaa tggttcggcg gctacccggc
      121 gcggcgcggc ttggcggtcg cgtaggcgta gtggaagcgg aaccccgact tcttctgggt
      181 cgtcacggcg tcgaggatcg cgcgcgagtg cgcatcgacc agatagcggg tcgtgatcac
      241 cggatcggca aagcgcacca cccacgcatc gacaccggtc tcccgcgtcc ggcccaggac
      301 agtgagcgtg tcgcgttcga gggcgccatc cacctcggag aagagcggca cgaccatctg
      361 cgccccgctc cggtactcga gcgatcgcac cagcggcttg acctcgttga acgcgaacac
      421 gggcacgtcg aaggtctggt cgaagacacg cggcgccgag tcaggatgct gaatggtgcc
      481 cgtcacgtgc gcgccggcgt atcgatacgc ataccgcacg tggttgaacg ccagcacctc
      541 ggaaaccggt cgcaggctcc gggcatcgac gatgagcgta tcgctggact ggaaaggcgg
      601 cgtccagttt gtcgttaggc ggataccgtc gtccgaccgg agcgccgcca tcgcgacgtg
      661 tgccacgaga cgcgtcgtcg tgtcgccggt gggacgctgc gtgaacacgg ccacgtcggc
      721 cgacgaatcg acgagattcg gcactctgag cgcgtgggcg ccaggcagca gcatgctgcc
      781 cggagcatgc ggcgaggtcg cgcacgcggc ggcgatcgcg acgcttgcgg cgatcgtcgc
      841 ttgtctcgct gtttgtcccg ctcgccgtgc ggtggtgggg acggcaacgg gtctcatcag
      901 acggccattt cagggaaggt gtgtcacact ctgctccgcc cgcacttccg atccaggcgc
      961 cggcgtgttg tagaagaccc agcggaccat gtatggcggc gcatcgatga tccacatgcg
     1021 actcgccagc ctgttggacc cgccagtgga tcccgcgacg taatcgtcca cgacccatgc
     1081 gcgacgatac aaggttccgc caacgcgaac gtgctcgcgg cctgtcgtgc ggtagagatg
     1141 tgtttcggtc gagtcattgc cctgccagag caccatacgg tagacgacgt tagtgcggcg
     1201 cggcaggact tcgacgacga gatccgctgc attgccaaag aacgccggcg tctcgagctt
     1261 gacatgaatg ggcgtggacg cgccgccact ctgacactgg gatccgtcaa tggacgtgcc
     1321 ttggaatcgc atcgtcccgc attgctggat gatggcgtag ggagccaggg tacggcgatc
     1381 gagaactgtc acaccggtgg cgcccccgct gttcgagctc atgaacatca aggggacgcc
     1441 ggcggaatct cgaaagacgg tcgtgaacgt cgcgtcgacc agcggcgtaa ccctaccagc
     1501 agtgatcttc gcgacgcgca cgcggccggc gta
//
LOCUS       DASUCJ010000810         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_345022, whole genome shotgun sequence.
ACCESSION   DASUCJ010000810 DASUCJ010000000
VERSION     DASUCJ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345022"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /gene="thrS"
                     /locus_tag="VFU90_09665"
     CDS             <1..>1045
                     /gene="thrS"
                     /locus_tag="VFU90_09665"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5480088.1"
                     /translation="GIVEDFIRAGLRESGYQPVVTPHVVSERLFEISGHLENFGENMF
                     GPLEVEEQRFRLKPMNCPGHIFIYKSRLHSYRDLPVRFSEFGTVYRFERSGVMHGLNR
                     VRGFTQDDAHLFCTPEQLQDEFEQTLDEAIRLMKAFDFTDFDYVLSSRERDARTETDV
                     AAENAIRKALERYELPYTVDEGGGAFYGPKLDINVRDAIGRKWQLGTVQVDFVLPKRF
                     DLKYRGADGRDHAPVMIHRALAGSLERFFGILIEHYGGAFPAWLAPVQAAVLPVSEHQ
                     LDYAREVAATLAAAGYRIDVDDSNEKLGYKIRRWKTAKVPYILVVGKQEAADGTVNVN
                     ERGIEEKRTLAISS"
BASE COUNT          211 a          333 c          319 g          182 t
ORIGIN      
        1 cggaatcgtc gaggacttca ttcgcgccgg gttgcgcgaa agcggctatc aaccggtggt
       61 gacgccgcac gtcgtcagcg agcgcctttt cgagatctcc ggacatctcg agaacttcgg
      121 cgagaatatg ttcggcccgc tcgaagtaga ggagcagcgc ttccggctca agccgatgaa
      181 ctgccccggc catatcttta tctacaagag ccggctgcac agctaccgag acctgccggt
      241 gcgtttcagc gagttcggca ccgtgtaccg cttcgaacgt tcgggcgtga tgcacggttt
      301 gaaccgggtg cgcggtttca cccaagacga cgcgcatctg ttttgcacgc ccgaacagct
      361 gcaggacgag ttcgagcaga cgctcgacga agcgatccgc ctgatgaagg cgttcgactt
      421 tacggatttc gactacgtgc tgtcgtcgcg cgagcgcgat gcccggaccg aaaccgacgt
      481 cgccgccgaa aacgcaattc gcaaagcgct cgaacggtac gagctgccct acaccgtcga
      541 cgaaggcggc ggcgcgtttt acggaccgaa actcgatatc aacgtgcgcg acgccatcgg
      601 ccgaaagtgg cagctcggca cggtgcaagt cgatttcgtt ctccccaaac gcttcgacct
      661 gaagtaccgc ggcgccgacg gacgcgatca cgcgccggtc atgatccacc gcgcgctggc
      721 cggctcgctc gagcgcttct tcggcatcct catcgagcat tacggaggtg cgttcccggc
      781 gtggctggca ccggtccaag cggccgtgct gccggtgtcg gagcaccagc tcgactacgc
      841 gcgcgaggtc gccgccacgc tcgccgccgc gggctaccgg atcgacgtcg acgacagcaa
      901 cgagaaactc ggctataaga tccggcgttg gaagactgcg aaggtcccct atatcttggt
      961 cgttggaaaa caagaagccg cggacggcac cgtcaacgtc aacgagcgcg gaatcgaaga
     1021 aaaacgcacg ctcgcgattt cttcc
//
LOCUS       DASUCJ010000811         1323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_54670, whole genome shotgun sequence.
ACCESSION   DASUCJ010000811 DASUCJ010000000
VERSION     DASUCJ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54670"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFU90_09670"
     CDS             complement(<1..543)
                     /locus_tag="VFU90_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5480089.1"
                     /translation="MQVSSVRRPRLLLVVAGVSLALLQACSSSGHANANPGQTTNAST
                     ASASTGGNPSSAPSSSGGSAAPTGSPLKIAIVAEMSGAGAATGQQWDDGVRWAIKQVN
                     AQGGVLGHPFEALTTLDTQTVPSVSIAAMKKALQDDPYAIMGTIYSSSTQVNAPEVGA
                     AGVPQFAGSQSDSLMNVSPTL"
     gene            complement(599..934)
                     /locus_tag="VFU90_09675"
     CDS             complement(599..934)
                     /locus_tag="VFU90_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5480090.1"
                     /translation="MFETSGAEPAFRMALDATSVTGRVILIGQNRYQTTLTSRELVQR
                     QITVSGSLIYDHPHDFATTVEACQTHADELAAVMAAAYKPAEAQRAFTEARNVAGKSW
                     LDLTAGWTR"
     gene            962..>1323
                     /locus_tag="VFU90_09680"
     CDS             962..>1323
                     /locus_tag="VFU90_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480091.1"
                     /translation="MQSSAEAFRESQPTFVRLDNECLDADGEQMRAKQQTLRSGTDHQ
                     TAITLTDARAPDCGPRDRVGFDARERNRRNAVVHRPTKRSGNADSLGRDAVFGDAKRA
                     PRHARRRCARLAWSAAEAA"
BASE COUNT          231 a          417 c          438 g          237 t
ORIGIN      
        1 cagcgtcggc gacacgttca tcagtgagtc cgactgtgag ccggcgaact gcggaacgcc
       61 cgcagcgccg acctccggcg cgttcacttg ggttgacgat gagtagatcg tgcccatgat
      121 cgcgtacggg tcgtcttgga gcgccttctt catggcggcg atcgacaccg acggcaccgt
      181 ctgcgtgtcc aaagtcgtga gggcctcaaa tgggtgaccc agcacgccgc cttgcgcgtt
      241 aacttgtttg atggcccacc taaccccgtc gtcccattgc tgaccagtgg cggcgccggc
      301 gccgctcatc tcggcgacga tggcgatttt gagcggagag cctgttggag ccgccgagcc
      361 gccggacgat gacggcgccg atgacgggtt gcctccggta gaagctgacg ccgttgacgc
      421 gttcgtggtc tggccggggt tcgcgtttgc gtggccggac gacgagcacg cctgaagaag
      481 agcgagggaa acgccggcga cgactaaaag caatcgcggc cggcgaacgc tgcttacctg
      541 catcgatggt cctctctggg atttcgttcg gctgtgtggg tcggatgagg gcggtcggtt
      601 agcgcgtcca tccagcggtg aggtctagcc acgacttgcc ggccacattg cgcgcttcgg
      661 taaaggcgcg ttgtgcttcc gcaggtttgt atgccgcggc catcacggcc gcgagctcgt
      721 cggcgtgcgt ttggcacgcc tcgacagtcg tcgcgaagtc atgtggatgg tcgtagatca
      781 atgagccact aacggtgatt tgacgttgaa ccagttccct gctcgtcaat gtcgtctggt
      841 agcggttttg cccgatgagg atgacccggc cggtgaccga cgtcgcatcg agcgccatcc
      901 ggaacgccgg ctcagcaccc gaggtttcaa acacaaagag gtacggctcc gagtcgggtt
      961 cgtgcagtcg agcgccgagg cgttccgcga gtcgcagccg accttcgtgc ggctcgacaa
     1021 cgaatgtctc gacgccgacg gagagcagat gcgcgcaaag caacagaccc tgcgatccgg
     1081 caccgatcac caaacagcga tcaccctgac ggatgcccga gcgccggact gcggtccgcg
     1141 cgaccgcgta gggttcgacg ctcgcgagcg caatcggcgc aacgccgtcg tccaccggcc
     1201 aacaaaacgc agcgggaacg ctgactcgct cggccgcgat gccgtgttcg gtgatgccaa
     1261 gcgcgcgcct cgccacgcac gacgacgttg tgcccgcctt gcatggtccg cagccgaagc
     1321 agc
//
LOCUS       DASUCJ010000812         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_131036, whole genome shotgun sequence.
ACCESSION   DASUCJ010000812 DASUCJ010000000
VERSION     DASUCJ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131036"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1572
                     /locus_tag="VFU90_09685"
     CDS             202..1572
                     /locus_tag="VFU90_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480092.1"
                     /translation="MKYAALLQFALIVLVAACAGQSPHSALPSNGAAAGQPSLRRGSS
                     TSPITHVIIILQENRTPDDLFQGMAPHGADIATQALDSEGQIVDLRRRSLATSFDLGH
                     GHSSYTDDCDFQPSGVCKMDGFDKNLEHRYHMRPFSYAPQSEVQPYLDMAVQYVFADR
                     MFQSNQAGSFPAHQYIVSGTADGLPVTSDNVSSDPFNSVTGAKVPAGCDAPAQAAVDT
                     IDPANGYPGPTPFPCFDRPVLSDLLDQYGFTWRYYQRGLGAGLWHALDAVSHVRYGSD
                     YKYVVTPPEKILTDITHKRLRNVSWVMPADGPHSDHAGNNSAAGPSWVAAIVNTLGSS
                     IYWKHTAIFLTWDDWGGWYDHVSPPQMGNYYELGFRVPLVVISPYAKAGYVSHTQYEF
                     GSILAFTEETFGIPKGALGTTDVRANDLMDAFDFAQTPRPYVQISAPPFVPAQSGTKG
                     FAAEDP"
     gene            complement(1594..2769)
                     /locus_tag="VFU90_09690"
     CDS             complement(1594..2769)
                     /locus_tag="VFU90_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5480093.1"
                     /translation="MTTAAPQIDEANEEERMMLAAVRELVAEKVAPRAAAIDESGDFP
                     WDVKELFAQNDLLGVPIPAEYGGLGGTFLTYVKVVEEIAKACASSSLIVAVQELGMLP
                     VMIAGTEEQKHRFLPKIASGEHLAAYALTEVSSGSDALGSMRTRAVRRDDAYVLNGQK
                     IWITNGSVADVVCVFAVTDPEAGSRGVSAFVVEKGTPGFTVGKKEKKMGIRGSPTVEL
                     SFADCVVPAANRLGDEGQGFKIAMQVLDKSRPGIAAQALGIAAGALDYATNYAKERVA
                     FGKPIGQHQGVGFMLADMKTEVEAARLLLYEAARRCDAGGPDVTLWAAMAKLKCGDVA
                     MSVTTDAVQILGGFGYSSEYPVERMMRDAKITQIYEGTQQIQRLVISRALVGKAKPG"
     gene            2862..3620
                     /locus_tag="VFU90_09695"
     CDS             2862..3620
                     /locus_tag="VFU90_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEU5480094.1"
                     /translation="MEKVYASCAGAVADIPDGASLAVGGFGLCGIPHNLIQALLEQGA
                     TNLVTVSNNCGVDGWGLGVLLDNRRIARTTGSYVGENKEFERQYLSGELEVELCPQGT
                     LAERLRAGGCGIPAFYTPAGVGTLVAEGGLPWRYGSDGSVAVASPKKEIRTFRGRDYV
                     LEEGIVCDYALVRASIGDRDGNLLFHKATRNFNPLCAMAGRITIAEVERLVEPGEIVP
                     EEVQLPGIFVQRVVEVGTEGKRIERVTTRARAGA"
     gene            3625..4281
                     /locus_tag="VFU90_09700"
     CDS             3625..4281
                     /locus_tag="VFU90_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEU5480095.1"
                     /translation="MALTRTQLATRLAQELRDGQYVNLGIGLPTLIPNYVPPGITVTL
                     QSENGILGMGPYPFEGEEDPDLINAGKETITLLPGASFFDSALSFGMIRGGHMDLAVL
                     GAMQVSQHGDLANWMIPGKMVKGMGGAMDLVHGAKRVIVMMEHVAKGNAFKIVRECSL
                     PLTGRRVVQKIVTDLGAIDVEPEGLVLRELAPGVTVEEIQAATEPRLRVPSPPAVMNV
                     "
     gene            4314..4565
                     /locus_tag="VFU90_09705"
     CDS             4314..4565
                     /locus_tag="VFU90_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480096.1"
                     /translation="MRLKTSKLTVLAFVFAMIFSAVAGGVAVAATQVHMRNALGDLQS
                     ALAQLQAANADKAGHREQAIKLVNSAIEQVNQGIAAGAQ"
BASE COUNT          831 a         1537 c         1509 g          854 t
ORIGIN      
        1 gcgtgaggcg gcggcgacga tcgggttctg cccggtaaac tccgggtcca tcggatctgg
       61 cacgtttcct cgttcccccg gcgagcattc gccgccggtc gtgcggcgcc cggtgtccga
      121 caaggccatc aacgcagggg gacgaagcat gaccggcggc ttcctaaggt gaccgcacgt
      181 gccggctaag gggtttgatc gatgaagtac gcagctttgc tgcagttcgc cttgatcgtg
      241 ctggtcgccg cgtgcgcggg acaatcgccg cactccgcgt tgccctcgaa cggagcggcc
      301 gccggccagc cgtcgctgcg ccgcggatcg tcgacgtcgc cgattacgca cgtcatcatc
      361 attctgcaag aaaaccgcac gcccgacgac ctatttcaag gtatggcccc gcacggcgcc
      421 gacatcgcca cgcaagcgct ggattccgaa gggcagatcg tcgacctgcg ccggcgttcg
      481 ctggcaacct cgttcgattt ggggcacgga cactcgtcgt acaccgacga ttgcgatttt
      541 cagccgagcg gcgtttgcaa aatggacggt ttcgacaaga acctggagca ccgctatcac
      601 atgcggccgt tctcctacgc gccgcagagc gaagtccagc cgtatctcga catggccgtt
      661 cagtacgtgt tcgccgaccg gatgtttcag agcaatcaag ccggaagttt cccggcgcac
      721 cagtacatcg tcagcggcac cgcggacggc ctgccggtaa cttccgacaa cgtatcgagc
      781 gacccgttca acagcgtgac cggcgcgaaa gtgccggccg gctgcgacgc gccggctcag
      841 gcggccgtcg acacgatcga tcctgccaac ggctatccgg ggccgacccc gtttccgtgt
      901 ttcgatcgtc cggtgctctc ggatctgctc gatcagtacg gcttcacgtg gcgctactat
      961 cagcgcggct tgggtgccgg gctgtggcac gcgctcgacg ccgtctcgca cgtgcgctac
     1021 ggctccgatt acaaatacgt cgtgacgccg ccggagaaga ttctgaccga catcacgcac
     1081 aagcgcttgc gcaacgtctc gtgggtaatg ccggccgacg gcccgcattc cgaccacgcc
     1141 ggcaacaata gcgcggccgg accgtcgtgg gttgccgcga tcgtcaacac cctcggctcc
     1201 agcatctact ggaagcacac ggcaattttc ctaacctggg acgactgggg cggatggtac
     1261 gatcacgttt cacccccgca gatggggaac tactacgagc tgggcttccg cgtgccgctg
     1321 gtcgtcatct cgccgtacgc caaggcgggg tacgtctcgc atacgcagta cgagttcggc
     1381 agcatcctag cgttcaccga agagacgttc ggaattccga aaggtgcgct cggaacgacc
     1441 gacgttcgcg ccaacgactt gatggatgcg ttcgatttcg cccagacgcc gcggccgtac
     1501 gtgcagatca gcgcgccgcc gttcgtaccc gcccagagcg gcaccaaagg gtttgcggcc
     1561 gaagacccgt agggcttgcc gattcggtag cggctagccg ggcttcgctt ttccgaccag
     1621 cgcgcgggag atcaccagcc gctggatctg ctgcgttcct tcgtagatct gcgtgatctt
     1681 ggcgtcgcgc atcatgcgct cgaccgggta ttcgctcgag tagccgaacc cgccgaggat
     1741 ttgcacggca tcggtcgtca ccgacatcgc gacgtcgccg cacttgagtt tggccatcgc
     1801 cgcccagagg gtgacgtccg gtccgccggc gtcgcagcgc cgcgcggctt cgtaaagcag
     1861 cagccgcgcc gcttcgactt cggttttcat atcggcgagc atgaagccga cgccttggtg
     1921 ctgcccgatc ggctttccga aagcgacgcg ttctttggcg tagttggtgg cgtaatcgag
     1981 cgcgccggcg gcgatcccga gcgcttgtgc ggcgatcccc ggacgcgact tgtcgagcac
     2041 ctgcatcgcg atcttgaagc cctgcccttc gtcgccgagg cgattggcgg cgggcacgac
     2101 gcagtcggcg aacgacagct cgaccgtcgg cgatccgcgg attcccattt tcttttcttt
     2161 cttgccgacc gtgaagccgg gcgttccctt ctccacgacg aaggcgctga cgccgcgcga
     2221 accggcttcg ggatcggtta ccgcgaaaac gcacacgacg tcggcgacgc tgccgttggt
     2281 gatccaaatc ttttgcccgt tcaggacgta tgcgtcgtcg cgacgcacgg ctcgcgtgcg
     2341 catcgatccg agcgcgtcgg agccgctcga aacttccgtg agcgcgtacg cggccagatg
     2401 ttcgcccgac gcgatcttcg gaagaaagcg gtgtttttgc tcttcggttc cggcaatcat
     2461 gaccggcagc atgcccagct cttgaaccgc tacgatcagc gagctcgacg cgcacgcttt
     2521 ggcaatctct tcgacgacct tgacgtaggt gagaaaagtg ccgcccaatc cgccgtactc
     2581 cgccgggatc ggcacgccta agaggtcgtt ctgagcgaaa agttccttga cgtcccacgg
     2641 gaagtcgccg ctctcgtcga tcgcggccgc gcggggagcc accttttccg cgaccagctc
     2701 gcggacggcg gcgagcatca tgcgctcttc ttcgtttgct tcgtcgatct gcggggcggc
     2761 ggtcgtcatc gtaacgttct ccttggtccg gccgatttcg cggttcgagc cgccttctca
     2821 ttggcaggac gaacgcgcgt actcccgcat aagcatcgag catggagaaa gtctacgcat
     2881 cctgcgccgg cgcggtggcc gacatccccg acggcgcctc gctggccgtc ggcggcttcg
     2941 gcctgtgcgg cataccgcac aacctgatcc aagcgctgct cgagcaaggc gcgacgaatc
     3001 tcgttaccgt ttcgaacaac tgcggcgtgg acggctgggg cttgggcgtg ctgctcgaca
     3061 accggcgtat cgcgcgtacg accggatcgt acgtgggtga gaacaaggag ttcgagcggc
     3121 aatacctctc cggcgagctc gaggtcgagc tgtgtccgca gggtacgctg gccgaacgct
     3181 tacgcgccgg cggctgcggg attccggcgt tttatacgcc ggccggcgtg gggacgctgg
     3241 tcgccgaggg cggactgccc tggcgatacg gctcggacgg cagcgtcgcc gtcgcctcgc
     3301 cgaagaaaga aatccggacg ttccgcggcc gcgactacgt gctcgaagag gggatcgtct
     3361 gcgactacgc gctggtccgg gcctcgattg gcgaccgcga cggaaacctg cttttccaca
     3421 aagcgacgcg caacttcaat ccgctgtgtg cgatggccgg gcggattacg atcgccgagg
     3481 tcgagcggct ggtggagccg ggggagatcg ttccggaaga ggtgcagctg ccgggcatct
     3541 tcgtgcagcg cgtcgtcgag gtcggtaccg aaggcaagcg gatcgaacgc gttaccacgc
     3601 gcgcgcgcgc aggagcgtaa actcatggca ctcactcgaa cgcagctggc gacgcgactc
     3661 gcccaagagc tccgcgacgg gcaatacgtc aacctcggga tcggtcttcc gacgctgatt
     3721 cccaactacg taccgccggg gattaccgtg acgctgcaaa gcgaaaacgg tatcctgggc
     3781 atggggccgt accccttcga gggtgaagaa gatcccgacc tgatcaacgc cggaaaagaa
     3841 accatcacgc tgcttcccgg cgcgtcgttc ttcgattcgg cgctgtcgtt cgggatgatc
     3901 cgcggcgggc acatggacct cgcggtactc ggcgcgatgc aggtttcgca gcatggcgac
     3961 ctggcgaact ggatgattcc cggaaagatg gtcaaaggca tgggcggcgc gatggacctg
     4021 gttcacggcg ccaagcgcgt tatcgtcatg atggagcacg tcgccaaagg caacgcgttc
     4081 aagatcgtgc gcgagtgttc gcttccgctc accggacggc gtgtggtgca gaagatcgtc
     4141 accgatttgg gcgcgatcga cgtggagccg gaggggctcg tgctgcgcga actcgcgccg
     4201 ggcgtgaccg tcgaggaaat tcaggccgcc accgaaccgc gcttacgggt cccgtccccg
     4261 cccgcggtca tgaacgttta atcaaacacc cactactaac aagaggagaa accatgcgtc
     4321 tcaaaacgtc gaaactcacc gtactcgcat tcgtctttgc tatgatcttc agcgcggtcg
     4381 ccggcggcgt cgccgtcgcg gccactcaag tgcacatgcg caacgcgctc ggcgacctgc
     4441 agtcggcgct ggcgcagctc caagctgcca acgccgacaa ggccgggcat cgcgaacagg
     4501 ccatcaagct cgtcaacagc gcgatcgagc aggtcaatca gggaatcgcc gccggcgccc
     4561 aatagctcgc cgggaaaacc cgcgtgcgag accggcgatc gagccggtct cgctttttta
     4621 tgcggacagg tcgcgaaact gggtgcggta gagccgcgcg tacagcccgc cgcgcgccag
     4681 cagctcggcg tggcggccgg tttccacgat ccgtcccgcg tcgacgacga a
//
LOCUS       DASUCJ010000813         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1322963, whole genome shotgun sequence.
ACCESSION   DASUCJ010000813 DASUCJ010000000
VERSION     DASUCJ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322963"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1470
                     /locus_tag="VFU90_09710"
     CDS             37..1470
                     /locus_tag="VFU90_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5480097.1"
                     /translation="MSGSLAKVEEQSAALRRPLALRDLVLTQLLYVVGSSWVGVAARL
                     GESQSAFWLLAILLFYLPQAAVVIYLSRRLPLEGGLYQWTKVGFGEFWGFLTAWNLWV
                     YTLVLLAATSLAVSSTLAYAIGPRATALAGTERFQLIVGVALIAMMVVVSVFGLRVGK
                     WVHNFGGLAQLVAFTALIIAPVVAAARGVHVAYRPLATGVPHVSLFSANVLGKLGMGA
                     LSGFEWVAIMAGESRDPGRSISRSVLVATPIIALMFILGTSATYSLVQGNAIDLVAPI
                     PQSLRLAYGSSGILGVVAPLLVMMLFVRAVANANLLFTGNTRLPMVAGWDGLLPSWFT
                     TLHPRWRTPVNSIAFVAAITFAILVFGVTGVGLQEAFQMLDTASGVFYALTYLAMFAL
                     PVLAASRLGGRAPRGLVIAAISGFLMTLLYIALSVVPIVGVVSRWAFAARIVFTIGIA
                     NLIGAGLYMVAARRRAARLAPSSADAA"
     gene            1501..2073
                     /locus_tag="VFU90_09715"
     CDS             1501..2073
                     /locus_tag="VFU90_09715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5480098.1"
                     /translation="MTTPLKVLGVSGSLRTGSYNRALLRAAIELMPPDMSIEEYNGIG
                     DLPLYNPDIDVQGGPATAVAWRTAVHAADALLIVTPEYNFGPSAALKNAIDWASRPPA
                     TSVLHAKPIALMGASTGMSGTTRAQLALRQSFVFTQSPVLSGPEVLVPYAATRFDAAL
                     RLTDAATRDFIHRHLVRFAEFARRLAPKTP"
     gene            complement(2060..>2644)
                     /locus_tag="VFU90_09720"
     CDS             complement(2060..>2644)
                     /locus_tag="VFU90_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480099.1"
                     /translation="VPVTPGQSATPVCRPNAFLPPGDTIPTGKPAPSMDALLTRVDSM
                     SGQLSLIVHSLQVELVQKNGVQDLHKTILSTNALLVELHRVAAQQSQALSMTLASIRR
                     SAAALDSAALDTTVHNVAATTRNLQAMTAELQKTSSRLDSVIGQVQHGDGTVSKLLND
                     PGVYNELRTLLARMDSLTADVKAHPKKYFNVKVF"
BASE COUNT          361 a          910 c          841 g          532 t
ORIGIN      
        1 tgcaccgctg atgcatccga ccgtggtgct tcgcgtgtga gtggatccct cgccaaggtc
       61 gaggagcaga gcgcggcgct gcgccgaccg ctggcgctcc gcgatctcgt tctcacgcaa
      121 ctgctctacg tcgtcggctc ctcctgggtc ggcgtcgcgg cgcggttggg tgaatcgcag
      181 agcgcgttct ggctcctcgc cattctgttg ttctatttgc cgcaggccgc agtggtgatc
      241 tatctcagcc gccggctgcc gctcgagggc gggctctacc aatggaccaa agtcggcttc
      301 ggtgaattct ggggatttct caccgcgtgg aatttgtggg tgtacacgct ggtgttgctc
      361 gccgcgacga gcctcgccgt gtcgagcacg ctcgcgtacg cgatcggacc gcgtgcgacg
      421 gcgctcgccg gcacggaacg ctttcaattg atcgtcggcg tcgccctcat cgcgatgatg
      481 gtcgtcgtgt cggtgttcgg cttgcgggtg gggaagtggg tgcacaactt cggcgggctc
      541 gcccaactgg tcgcgttcac cgcgctcatc atcgcgcccg tcgtagcggc ggcgcgcggc
      601 gtccacgtcg cgtatcggcc gctcgccacc ggcgtcccgc acgtctcgct cttcagcgcc
      661 aacgtgctgg gcaagttagg catgggcgcg ttgagcggct tcgagtgggt cgccatcatg
      721 gccggcgaat cgcgcgaccc cggtcgcagc atttcgcgct ccgtgctcgt cgccacgccg
      781 atcattgcgc tcatgttcat cctggggacg agcgccacgt actcgctcgt ccagggcaat
      841 gcgatcgacc tcgtggcgcc gattccgcag tcgctgcggt tggcctacgg gtcgtcgggc
      901 atcctcggcg tcgtcgcccc gctcctcgtg atgatgctct ttgtgcgcgc cgtcgccaac
      961 gcgaacttgt tgttcacggg caatacgcgt ctgcccatgg tggccggctg ggatgggttg
     1021 ctcccgtcgt ggttcaccac gctgcatccc aggtggcgga cgccggtcaa ctcgatcgcg
     1081 ttcgttgccg cgatcacgtt cgcgatcctg gtgttcggcg tcaccggcgt cggtttgcag
     1141 gaagccttcc agatgctcga cacggcgtcg ggcgtgttct acgcgctcac ttatctggcg
     1201 atgttcgcgc tgcccgtgct ggccgcgtct cggttaggcg gacgcgcgcc gcgcggcctg
     1261 gtgatcgcgg cgatctccgg cttcctcatg accttgctgt acatcgcgtt gtccgtcgtg
     1321 ccgatcgtcg gcgtcgtcag ccgatgggcc tttgccgccc gcatcgtgtt cacgatcggc
     1381 atcgccaacc tcatcggcgc ggggttgtac atggtcgccg cgaggcgacg cgccgcgcgc
     1441 ctggcgcctt cgagcgccga tgccgcctag atttcccgca actcgacgca tcgcaacacc
     1501 gtgacgactc cgctcaaggt cctcggcgta tccggcagtc tgcgcaccgg ctcgtacaat
     1561 cgcgcattgc tgcgggccgc gatcgagttg atgccgccgg acatgagcat cgaggaatac
     1621 aatgggatcg gcgatctgcc gctctacaac cccgacatcg acgtccaggg cggaccggcg
     1681 accgccgtcg cttggcgcac ggccgtccac gccgcagacg cgctcctgat cgtgactccc
     1741 gaatacaact tcggacccag tgctgcgctc aagaatgcga tcgattgggc gtcgcgtccg
     1801 ccggcgacct cggtgctgca cgccaagccc atcgcgctca tgggcgcctc gaccgggatg
     1861 tcgggcacga cgcgcgcgca gttggcgctg cgccagtcgt tcgtgttcac gcaatcgccg
     1921 gtgctgtccg ggcccgaggt gctggtgcca tatgccgcga cgcgtttcga cgccgccctt
     1981 cgccttaccg acgccgccac ccgcgacttc atccaccgcc acctggtccg gttcgcggag
     2041 ttcgcgcgcc gactcgcgcc taaaacacct tgacgttgaa gtactttttc ggatgtgcct
     2101 tcacatccgc cgtcaaggaa tccatccgcg ccaagagcgt gcgaagctcg ttgtacacgc
     2161 ccgggtcgtt cagcagcttg ctcaccgttc cgtcgccgtg ctggacttgt ccgatcaccg
     2221 aatccagtcg cgacgacgtc ttctgaagct cggccgtcat cgcctggagg ttgcgcgtcg
     2281 tcgccgcaac gttgtgcacg gtcgtgtcca gcgccgcgct gtccagtgct gccgcgctcc
     2341 ggcgaatcga cgccagcgtc atcgacaacg cctgcgattg ttgggccgcc actcgatgca
     2401 gctccaccaa cagcgcgttg gtcgacagga tggtcttgtg gaggtcctgc acgccgttct
     2461 tctgcaccag ctccacctgc aacgaatgga cgatgagcga caactgcccg ctcatcgagt
     2521 ccacgcgcgt caacagcgca tccatgctcg gcgccggctt gcccgtgggt atggtgtcgc
     2581 ccggcggcag aaacgcgtta ggccgacaga ccggcgtcgc actttggccc ggcgtcaccg
     2641 gaac
//
LOCUS       DASUCJ010000814         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_497313, whole genome shotgun sequence.
ACCESSION   DASUCJ010000814 DASUCJ010000000
VERSION     DASUCJ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497313"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VFU90_09725"
     CDS             complement(<1..1424)
                     /locus_tag="VFU90_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480100.1"
                     /translation="MTIKRFFGLLAATTFGAASLLSTLPASAQVVYAPSAGAPISPYT
                     PILASLEPQGCPLYGNGQWAFINNVWIWCPPTAVAVAPPVGQAALSGYYGFGATMPPP
                     TIAVAPPQAIAYAPVYGSSFGAPSYATPYPYGTYGSPYAYGTPVAYIPPYGYGYPAPA
                     PAAYPYPYGGGYGYPQQYPQTAYPYPYGGGYGYPQPYPQQYPYPYGGGYPGGYGYPGG
                     GYGYPGGGYGYPGGGYGSLTGSPLLDMLIGVVATGLISSALQPSYNQGYGYPGYGYGA
                     PSYGYGAPSYGYQGYGYPYAQNYPVAYQNPGYGYGNGYGYGGGGNTYYYTKNVYKYYG
                     NGGNKGGSGSGKQPRIMHPYFHPINNELTWIARDRARIAMLRGEGGHAAQIAQLKRNI
                     WRDQNRLARLRTERNRFDQHVAGRNLVRANAPRLAAAHVVAPRMITAHVTAPRMTTAH
                     VTAPRMTTAHVIAPRMTTAHVVAPR"
     gene            complement(1510..1917)
                     /gene="mce"
                     /locus_tag="VFU90_09730"
     CDS             complement(1510..1917)
                     /gene="mce"
                     /locus_tag="VFU90_09730"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU5480101.1"
                     /translation="MEIDHVAVVVKDLDAAVFLYTQTLGFREIYREVIADQGVEAVGL
                     AAGSAVVELLRPLDEQSPIARYRGDAPTRLHHTAYRVADLQAKLDELKAAGVRLLDEQ
                     PRRGAHGNLIAFLHPKSTAGVLIELCQPPPGRF"
     gene            complement(1917..>2390)
                     /locus_tag="VFU90_09735"
     CDS             complement(1917..>2390)
                     /locus_tag="VFU90_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5480102.1"
                     /translation="GSYYVEALTAELIERGRALIAEVDALGGSVAAIESGWMQARIAD
                     SAYAAQQAIERGDAVVVGVNAFADAEEAGASIPLQRIDERIEREQCERVRAARAGRDA
                     ELVNRRLAAVRAAAAGTENLMPHFIEAVDAGATLGEICDVLRDVFGVYRAKEIVA"
BASE COUNT          400 a          753 c          840 g          397 t
ORIGIN      
        1 cgcggagcga cgacgtgggc cgtggtcatg cgcggagcga tgacgtgagc cgtggtcatg
       61 cgcggagcgg tgacgtgggc ggtggtcatg cgcggagcgg tgacgtgggc ggtgatcatg
      121 cgcggagcga cgacgtgggc agctgccagg cgcggggcat tcgcgcgaac cagattgcgt
      181 ccggcgacgt gctggtcgaa gcggttacgc tccgtccgca ggcgcgccaa gcggttctgg
      241 tcgcgccaga tgttgcgctt cagctgagcg atctgagctg cgtggccgcc ttcgccgcgg
      301 agcattgcga tgcgagcgcg atcgcgggcg atccaggtaa gctcgttgtt gatcggatgg
      361 aagtacggat gcatgatgcg cggctgcttg ccggatcccg atccgccctt gttcccaccg
      421 ttgccgtagt acttgtacac gtttttcgtg tagtagtacg tgttgccgcc gccgccgtat
      481 ccgtagccgt tgccgtagcc gtagcccgga ttttggtacg cgaccggata gttctgggcg
      541 tacgggtagc cgtagccctg atagccgtag ctcggggcac cgtaaccgta gctcggggca
      601 ccgtagccgt agcccgggta gccgtagccc tgattatacg aaggctggag ggccgacgag
      661 atcagacccg ttgcgaccac gccgatcagc atgtccagca gcgggctgcc ggtcagcgaa
      721 ccgtagccgc cgcccggata gccgtagccg ccgcccgggt aaccgtagcc gccgcccgga
      781 taaccgtagc cgccgggata tccgccgccg taaggatacg gatactgttg cgggtagggc
      841 tgcgggtaac cgtagccgcc gccgtaagga tacgggtacg cggtctgcgg atactgctgc
      901 ggataaccgt agccgccgcc gtacggatat ggatacgcgg ccggtgccgg cgcgggatac
      961 ccgtaaccat aaggcgggat gtacgcgacg ggcgttccgt acgcgtaggg cgatccgtac
     1021 gtcccgtagg ggtacggcgt cgcatagctg ggagctccga acgacgatcc gtaaaccggc
     1081 gcgtaggcga tggcctgcgg cggtgcgacg gcgatcgtcg gaggcggcat cgtggcgccg
     1141 aaaccgtaat aacccgacag cgccgcttgc ccgactggag gtgcgacggc aaccgcggtc
     1201 ggcggacacc agatccaaac gttattgatg aacgcccact ggccgttgcc gtacagcggg
     1261 cagccttgcg gctctaagga cgccagaatc ggcgtatatg gagaaatcgg tgcgcccgcc
     1321 gaaggcgcat agaccacttg ggcgctagcg gggagcgtcg agagcagcga tgccgcaccg
     1381 aacgtcgtgg ccgccaaaag cccgaagaac ctcttgatag tcatatgttg catgtacctc
     1441 tgtgccgatt gtgacgtaca tcggcccgag gcacgcgtgg attacgatta ggcggccgtt
     1501 aaagcggggt tagaagcgcc ccggcggcgg ctggcacaac tcgatcagca cgccggccgt
     1561 cgactttgga tgcaggaagg cgatcaagtt tccgtgcgcg ccgcggcgcg gctgctcgtc
     1621 gagcaggcga acgccggcgg ctttgagctc gtcgagcttc gcctgaagat cggctacccg
     1681 gtacgcggta tggtgcaagc gcgtgggggc gtcgccgcga tagcgcgcga tcggcgattg
     1741 ctcgtcgagc gggcgcagca gctcgacgac ggccgaaccg gcagccaagc cgactgcctc
     1801 gacgccttga tcggcaatca cctcgcgata gatctcgcga aacccgagcg tctgcgtata
     1861 gaggaagacc gccgcgtcga gatctttgac gacgacggcg acgtggtcga tctccactac
     1921 gcgacgatct ccttggcacg atagacgccg aatacgtccc gtaagacgtc gcagatctcg
     1981 ccgagcgtag caccggcgtc gaccgcctcg atgaagtgcg gcatgaggtt ctcggtgccg
     2041 gccgcggccg cgcgcaccgc cgcgagccgc cggtttacga gctcggcgtc gcgccccgcg
     2101 cgggcggcgc gcactcgctc gcactgctcc cgctcgatcc gctcgtcgat tcgctgcagc
     2161 gggatcgaag cgccggcttc ttcggcgtcc gcaaaggcgt tgacgccgac gaccaccgcg
     2221 tcgccgcgct cgatcgcctg ctgcgcggcg taggccgagt cggcgatgcg cgcttgcatc
     2281 cagccgcttt cgatcgcggc gacgctgccg cccagcgcgt cgacctcggc gatcagcgcg
     2341 cggccgcgct cgatcagctc ggcggtgagc gcttcgacgt agtacgaacc
//
LOCUS       DASUCJ010000815         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_711739, whole genome shotgun sequence.
ACCESSION   DASUCJ010000815 DASUCJ010000000
VERSION     DASUCJ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711739"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFU90_09740"
     CDS             complement(<1..780)
                     /locus_tag="VFU90_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEU5480103.1"
                     /translation="MAERRIDRWLRPVIVLPVLGALLLAAVIFSPGGFTSSVDRRLTT
                     YSTTPFGARALYEVLGRLGWHVVRRRSAFRSPMDSTDTYLILSPPYDASATEVSALLD
                     AVRRGATIVVSPDAGSALADSLRIRRTRFDQDLEVLRSAATDASDSASSFIAAAINAG
                     GFSRYLRPVATSERDTEPVFPSDTMTLLRVRADSGRVQPAVMVRKLGNGAAVVVADLS
                     FAQNDNVRDTTGMILAVRLLELAGIDRRHRLVFDEYHQGFGD"
     gene            complement(773..>1098)
                     /locus_tag="VFU90_09745"
     CDS             complement(773..>1098)
                     /locus_tag="VFU90_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU5480104.1"
                     /translation="GMRRSGGDDPWTAAETLAARGDYTSAAHALYAAMLESAARQQQI
                     RLHPSKTVGDYVRELRTHSSSLFTRFREFGRSYEVVIYGIGTCDAARYHQLYALAVPV
                     VRPNG"
BASE COUNT          198 a          390 c          356 g          154 t
ORIGIN      
        1 gtcgccgaag ccctggtgat actcatcgaa caccagacgg tgcctccgat cgatccccgc
       61 gagttcgagc agccgcaccg cgagaatcat cccggtggtg tcgcgcacgt tgtcgttctg
      121 cgcgaaggag aggtccgcca cgacgaccgc cgccccgttg cctaacttgc gaaccatgac
      181 ggcgggctgc acgcgcccgc tgtcggcgcg cacacggagc agcgtcatcg tatccgacgg
      241 aaacacgggc tccgtgtcgc gctccgacgt agccaccggc cgcaagtaac gggagaaccc
      301 gcccgcattg atggcggcgg cgatgaacga gctcgcgctg tccgacgcgt cggtcgcagc
      361 cgatcgcagc acctcgagat cctggtcgaa gcgcgtgcgc cggatgcgca gcgagtccgc
      421 cagcgccgac cccgcgtccg gcgagacgac gatggtcgcg ccgcgacgca ccgcgtctaa
      481 caatgcggac acttcggtgg cgctggcgtc gtacggcggc gacaaaatca ggtaggtgtc
      541 ggtcgagtcc attggtgacc ggaacgcgct gcgccggcgc acgacgtgcc aaccgagtcg
      601 cccgagaact tcatacaggg cccgcgcacc gaacggcgtc gtgctgtacg tcgtgaggcg
      661 cctgtcgacc gagctcgtga aaccgcccgg cgaaaaaatc accgccgcca gcaagagcgc
      721 gccgagcacg ggcagcacga tcaccggtcg cagccaccgg tcgatgcggc gctcagccat
      781 tgggacgaac caccggtacc gcaagcgcgt acaactggtg gtagcgtgcg gcatcgcacg
      841 tcccgatgcc gtagatcacg acttcgtacg agcggccaaa ttcacgaaac cgcgtgaaca
      901 gcgaggacga atgcgtgcgc agctcgcgca cgtaatcgcc caccgtcttc gacgggtgca
      961 accggatctg ctgctggcgt gcggcgcttt ccagcatcgc cgcgtacaac gcgtgcgccg
     1021 cgctcgtgta gtcgccccgt gcggccagcg tctccgccgc cgtccacgga tcgtcgccgc
     1081 cggacctgcg catcccgc
//
LOCUS       DASUCJ010000816         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1002076, whole genome shotgun sequence.
ACCESSION   DASUCJ010000816 DASUCJ010000000
VERSION     DASUCJ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002076"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFU90_09750"
     CDS             <1..193
                     /locus_tag="VFU90_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480105.1"
                     /translation="NYGLDDVRFPAPLTIPSRIRAHFKVAEVAPKSDGTKSVVKVSVE
                     SEGVEKPVCVADMLLLHYR"
     gene            203..841
                     /locus_tag="VFU90_09755"
     CDS             203..841
                     /locus_tag="VFU90_09755"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5480106.1"
                     /translation="MRLGASMEATVAVDDRGVIVAWNPAAEKLLGRLASEAIGMQCHE
                     VMHGKSPAGAPICQQDCAIVQLCREGRAARRYEMIARRPDGSELWLDVTSVTVFDDGR
                     PVAVHVLTESVTSSRLTTLAEDVARRLTAGHTDEGDKAAAIKIRQALTKRELEVLQLL
                     AAGLDTDSIAGQLGLTRATVRNHVQNLLPKIGAHTRIEAAVLALKAGLVHLH"
BASE COUNT          201 a          346 c          331 g          162 t
ORIGIN      
        1 caactacggg ctcgacgacg tgcgctttcc cgcgccgctg acgataccgt cgcgcatacg
       61 agcgcacttc aaggtcgccg aggtcgcgcc gaaatccgac ggaaccaaat cggtcgtcaa
      121 ggtctccgtc gaaagcgagg gcgtcgagaa gcccgtgtgc gtcgccgaca tgctcctgct
      181 gcactatcga taggagacgg gcgtgaggct aggagcgagc atggaagcca ccgtcgcggt
      241 cgacgaccgc ggcgtcatcg tcgcctggaa cccggccgcg gagaagttgt tgggcagact
      301 cgcgtccgaa gccatcggaa tgcaatgtca cgaggtcatg cacggcaagt cccccgccgg
      361 cgcgccgatc tgccagcagg attgcgcgat cgtccagctc tgtcgcgaag gcagggccgc
      421 gcggcgttac gagatgatcg ctcgacggcc ggacggcagc gagttatggc tcgacgtcac
      481 ttccgtcacg gtcttcgacg acggtcggcc ggtggcggtc cacgtcctga cggagagcgt
      541 cacgagcagc cgcctcacga cccttgcgga agacgtcgcc cgccgtctga ccgccggcca
      601 cacagatgag ggtgacaaag ccgcggcgat caagatccgt caagcgctca cgaaacgcga
      661 gctcgaggtg ctccaactcc tcgccgccgg cctcgacacc gactcgatcg ccggtcagtt
      721 gggtctcacc cgcgcgacgg tgcgcaatca cgtccagaac ctgttgccga agatcggcgc
      781 gcacacgcgc atcgaagccg ccgtcctcgc attgaaagcc ggcctagtgc acttgcatta
      841 gataacccgc gcagacgctt cttgcggcac gcgatgctcg cggcctatca tgttttcgaa
      901 cgaacgctcg aacgaaaagc gtcgttcgga gcacttggcc gatgaaagca ttcgagtcac
      961 gccttcggac cgcactggac gcggccgagc gcgtcttcgc cgagaaggac tacggcgatg
     1021 cgacgatgcg cgacgtcgcc
//
LOCUS       DASUCJ010000817         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_146515, whole genome shotgun sequence.
ACCESSION   DASUCJ010000817 DASUCJ010000000
VERSION     DASUCJ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146515"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFU90_09760"
     CDS             complement(<1..801)
                     /locus_tag="VFU90_09760"
                     /inference="COORDINATES: protein motif:HMM:NF044398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5480107.1"
                     /translation="MTGTAKTEEREFRDIYGLDVVVVPTNMPMVRKDNSDIVFKSEKA
                     KFEAVVDEIIGEHEKGRPVLVGTRSIEKSELLAAMLRRRGVECNVLNAKYHEQEAEIV
                     KDAGQEAQVTIATNMAGRGTDIKLGDGVAKNGGLHIIGTERHESRRIDNQLRGRSGRQ
                     GDPGSTRFYISLEDEVMRLFGGERMTAIMDRVGFTDEQPIESGLVTKSIERAQSKVEN
                     HNYEIRKHVLEYDDVMNKQRSIIYADRRAILEGKFDSRTFMLQTLEAKV"
     gene            complement(775..1566)
                     /locus_tag="VFU90_09765"
                     /pseudo
     CDS             complement(775..1566)
                     /locus_tag="VFU90_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          239 a          496 c          470 g          361 t
ORIGIN      
        1 gactttcgcc tcgagcgtct gcagcatgaa cgtgcgcgag tcgaacttgc cctcgagaat
       61 cgcacggcga tcggcgtaga tgatcgagcg ctgcttgttc atcacgtcgt cgtactcgag
      121 aacgtgttta cggatctcgt agttgtggtt ttccaccttg ctctgcgcgc gctcgatcga
      181 cttggtaacc agtcccgatt cgatcggctg ttcgtcggtg aagccgacgc ggtccattat
      241 cgccgtcatc cgctcgccgc cgaataggcg catcacttcg tcttcgagcg aaatgtaaaa
      301 tcgcgtcgat cccgggtcgc cctgccggcc ggagcggccg cgcagctggt tgtcgatccg
      361 gcgcgattcg tgacgctcgg ttccgatgat gtgcagtccg ccgttcttgg cgaccccgtc
      421 tccgagtttg atgtcggtgc cgcggcccgc catgttggtc gcgatcgtca cctgggcttc
      481 ttgcccggcg tctttgacga tctcggcttc ttgctcgtgg tatttggcgt tgagcacgtt
      541 gcattcgacg ccgcggcggc gcagcatcgc cgcgagcagc tcgctttttt cgatcgaacg
      601 cgtgccgacc agcacggggc ggcccttctc gtgctcgccg atgatctcgt cgacgaccgc
      661 ctcgaatttc gctttttccg acttgaacac gatgtccgag ttgtctttgc gaaccatcgg
      721 catgttcgtc ggaacgacca ccacgtccaa accgtagatg tcgcggaact cgcgctcctc
      781 ggtcttggcg gtgccggtca ttccggccag atgctcgaac agccggaaca gattttggaa
      841 cgtgatggtc gccagcgtct ggtcctcgcc gcggaccttg attccttcct tcgcttcgat
      901 cgcctggtgg ataccgtcgg agtagcgccg tccgtacatc agccggccgg tgaactcgtc
      961 gacgataatc acctcgccgt ctttgacgat gtactgctgg tcgcggtgga acagattcca
     1021 cgctttgagc gcggcgttga gctggtgggc cagctcgatg ttgcgctgat cgtagagatt
     1081 ctggatgccc agcatcttct cgaccttggt aacgcccggc tccatgatcg ggacggcgtg
     1141 cgccttctcg tcgaccgtga aatcctcgcc ctttttcagg cgcggaatga tctgcgcgaa
     1201 tttttcgtac agctcgcccg attcttgtga cggcccgctg atgatcagcg gcgtgcgcgc
     1261 ttcgtcaacc agaatcgagt cgacctcgtc gaccagcgcg aaatacagct cgcgctgcac
     1321 caggtcttcg acctgccacg ccatgttgtc gcgcaggtag tcgaacccga cctcgttgtt
     1381 ggtgacgtag gtgacgtcgc agttgtaggc cgcgcggcgt tgcgccgagt cgagcccgtg
     1441 ctggatgatc cccacgctca gcccgaggaa ctcctacagc gggcccatcc actcggcgtc
     1501 gcgccgggcg agataatcgt tgacggtgac gaccgtcacg ccgcggcctt cgagcgcgcg
     1561 cgcgta
//
LOCUS       DASUCJ010000818         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1063296, whole genome shotgun sequence.
ACCESSION   DASUCJ010000818 DASUCJ010000000
VERSION     DASUCJ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063296"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VFU90_09770"
     CDS             complement(<1..867)
                     /locus_tag="VFU90_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480108.1"
                     /translation="MTRRLVVSLLFALVQTACSGGGSQTGGSLLPGPKATPTPFPAPS
                     YIKHLVIMIQENRSYDDFFATYPGADGTTTGLTHTGQKVPLKEVGLISFDLDHTWQGY
                     QLEYDGGKMDGFDLVHLGAWGGGPPAGLYAYQYVKPADIQPYWTMAQQYVLADHMFET
                     QSSGSFIGHQDLIAGGTAINSTQSLVNYPNSRIWGCDAPKATHTSLITSDHQFLRGAG
                     PFPCLTYPTLRDSLDAGNVSWKYYVPTFSTIGRLWNAFDAIDAVRHGPEWNTNIAAPE
                     TRVFKDISDGKLP"
     gene            942..1787
                     /locus_tag="VFU90_09775"
     CDS             942..1787
                     /locus_tag="VFU90_09775"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5480109.1"
                     /translation="MRSNDAAGELRALHAGERVLILPNVWDAASAALFAAAGAGALAT
                     TSAGLAWSCGYPDGDALPRASLLFALGAICRVSGKLPVSADVEGGFSQDPETVADLVR
                     ELCALGVGGINLEDGGADPDVLSAKIRAVKRSVAACGGDVFVNARTDVYLREMATGDA
                     AVNEVVARARRYAAAGADGIFVPGLAAPAEIAAIASAVDLPLNLMAIPQLSPVRELYA
                     LGVRRLSAGDALAARAYGAARAAAEAFLAGGDAAVLFSEPAVDYASTNALLAGAAAAA
                     PAPRP"
     gene            complement(1792..>2652)
                     /locus_tag="VFU90_09780"
     CDS             complement(1792..>2652)
                     /locus_tag="VFU90_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEU5480110.1"
                     /translation="VANAHSVDVYDVQDWTRLRRLGNPNPRALAFDRQGNLYVADDHA
                     NTVTVYARGKTTRLRTIKAGLDAPNSMAFDSSGNLYVSNNNNKSVTVYAPGSGAVLRT
                     ITDGINGPHDVIFDASGNLYVGNNGSSHITVYPPGGTHVIRTIITHNGPLALAFDGAG
                     DLFCVNNFNVTVYKPGKTQPDRTITNGISLPSTLAFDPAGNLYVANWGTNMFRSTIAV
                     YGPGGSTPHKTIGDGVGYPLSLVFDNAGRLYVANSGFNTVTIYAPGASKPERTLTNGI
                     VAPRVLTFGP"
BASE COUNT          417 a          819 c          912 g          504 t
ORIGIN      
        1 cggcagcttt ccgtcggaga tgtccttgaa taccctcgtt tcgggtgcgg cgatgttggt
       61 gttccattcc gggccgtggc gcaccgcgtc gatagcgtcg aacgcattcc aaaggcggcc
      121 gatcgtcgag aacgttggga cgtagtactt ccacgacacg ttcccggcgt cgagcgagtc
      181 gcgaagcgtc gggtaggtca agcacgggaa gggtcccgcg ccccgcaaga actgatggtc
      241 gctggtgata agcgacgtat gggttgcctt cggcgcatcg catccccaga tccggctgtt
      301 gggatagttc accaaacttt gggtcgaatt gatggccgtt ccgccggcga tcagatcctg
      361 gtggccgatg aagcttccgc tgctttgggt ttcgaacatg tggtccgcca agacgtattg
      421 ctgcgccatc gtccagtacg gctgaatgtc ggccggcttg acgtattgat aggcatacag
      481 cccggccggc gggccgccgc cccacgcgcc gagatggact aaatcgaaac cgtccatttt
      541 tccgccgtcg tattcgagtt ggtagccttg ccaggtgtga tccaaatcga acgagatcag
      601 tccgacctct ttgagcggca ccttctgtcc cgtgtgcgtc aatccggtgg tggtcccgtc
      661 ggcgcccgga taggtcgcga agaaatcgtc gtaactgcgg ttctcttgga tcatgatgac
      721 gaggtgcttg atgtacgatg gcgcaggaaa gggcgtcggc gtcgccttgg gacccggcag
      781 gagcgagccg ccggtttggg acccgccgcc ggaacaagcc gtttggacga gcgcgaagag
      841 cagggacaca acgaggcggc gtgtcatcgt tgagacggaa taccgcccgg agaacggtcc
      901 ttccccccgg ttccggcaga ccaagcggag tcaacgatac gatgcgatcc aacgatgcag
      961 ccggcgagct gcgcgcactg cacgcgggcg agcgcgtcct cattctcccc aacgtgtggg
     1021 atgcggcatc cgccgcgctg tttgcggccg ccggagcggg agccctcgcg acgacgagtg
     1081 cgggcctcgc gtggtcgtgc ggttaccccg acggcgatgc gctgccgcgc gcaagcttgc
     1141 tgttcgccct tggtgcgatt tgccgcgttt ccggaaagct tcccgtgagc gccgacgtcg
     1201 aaggcggatt ttcgcaggac cccgagacgg ttgcggatct cgtgcgcgag ctgtgcgcgc
     1261 tcggcgtcgg cgggatcaat ctcgaagacg gcggcgcgga tccggacgtg ctgtcggcca
     1321 agattcgtgc ggtaaaacgc agcgtcgccg cgtgcggcgg cgacgttttc gtcaacgcgc
     1381 gaacggacgt gtatttgcgc gagatggcaa ccggcgacgc ggccgtgaac gaggtcgtgg
     1441 cgcgcgcgcg ccggtacgcc gcggcgggcg ccgacggcat cttcgttccc gggctggcgg
     1501 cgccggccga aattgcggcg atcgcgagcg ccgtcgattt gccgctcaat ctcatggcga
     1561 ttccgcagct ttcgccggtg cgggaactct acgcgctggg cgttcggcgc ttgagcgcgg
     1621 gcgacgcgct cgcggcgcga gcatacggtg ccgcacgggc cgccgccgaa gcgttcctgg
     1681 ccggcggcga cgccgccgtt ctgttctccg agccggccgt cgactacgcc tcgaccaacg
     1741 cgctgctcgc cggcgccgcg gctgccgcgc ccgcgccgcg gccgtagccc gttacggtcc
     1801 gaacgtcaga actctcggcg cgacgattcc gttcgtcagc gtgcgctcgg gcttcgacgc
     1861 ccccggagcg taaatcgtga cggtgttgaa gcccgagttg gcgacgtaga gccggccggc
     1921 gttatcgaac accagcgaga gcggataccc gacgccgtcg ccgatcgttt tgtgcggcgt
     1981 ggaaccaccg ggcccgtaca ccgcgatcgt cgagcggaac atgttggtgc cccagttggc
     2041 gacgtacaga ttaccggcgg ggtcgaacgc cagcgtgctg ggcagactta tcccgttggt
     2101 gatcgtccgg tccggctggg tctttcccgg cttgtacacc gtgacgttga agttgttgac
     2161 gcagaacagg tcgccggcac cgtcgaaggc cagcgcgagc ggaccgttgt gcgtgatgat
     2221 cgtgcgaatg acgtgggttc cgcccggcgg gtagaccgtg atgtgactcg agccgttgtt
     2281 tcccacgtag aggtttccgc tcgcatcgaa gatcacgtcg tgcggaccat tgatgccgtc
     2341 ggtgatcgtc cgcagcaccg cgccgctccc cggggcgtaa accgtgacgc tcttgttgtt
     2401 gttgttggat acgtacaggt ttcccgacga gtcgaacgcc atcgagttcg gagcatccaa
     2461 gcccgccttg atggtacgca gccgcgtcgt cttgccgcgc gcgtagacgg tcaccgtatt
     2521 ggcgtggtcg tcggcaacgt agagattgcc ttggcggtcg aatgcgagtg cacgcggatt
     2581 tgggttgccg agccggcgca gccgcgtcca gtcttggacg tcgtagacgt ccaccgaatg
     2641 cgcgttggcg ac
//
LOCUS       DASUCJ010000819         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1093863, whole genome shotgun sequence.
ACCESSION   DASUCJ010000819 DASUCJ010000000
VERSION     DASUCJ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093863"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFU90_09785"
     CDS             complement(<1..1322)
                     /locus_tag="VFU90_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480111.1"
                     /translation="MRSVVALLLALCVRHITAAAQQPDTTRRDTTRADTTARRLAKVV
                     VTGTRLTDVDERIPSQVESMDLQHISQGPDAAFRSLQQLPGVTFFDDQGTRLQPELEV
                     RGFQISPIVGTPQGVSVFLDGVRVNEPDAQEINFDLLPMAAIDHAELVRGSQALFGRN
                     SLGGALLFFTQRGMPTPSANIELTTGSFGEQVGTISAGGMSHGIDGFIMGSASNEDGW
                     RQATGARTRQLFATVGHRSSSGGPDTSDIALSVLYANNRISEAGSLPETWIQSNPRLN
                     YTPGDFFKPDLIHVALRGFRGAWGGTLRGTLFFRRNDIEQFNVNVPPPSTDAFIDNKS
                     GGGTAEWTRPLLLASLPTAFTIGAEYARQNVRFRLTNAGGGTSDSVATLAVVHEDDAA
                     AYAQAVVDLTPKLSATAAVRQDYVRIPFRDELDPANNGTNTYDRLSPFV"
     gene            complement(1319..>1716)
                     /locus_tag="VFU90_09790"
     CDS             complement(1319..>1716)
                     /locus_tag="VFU90_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480112.1"
                     /translation="RSFVPAAPSRSVRHRHGMGLALATLVASAAAIALWMGARPTTHA
                     SASGVYRMHDVCCFNLDGGERADDGMLTVSRAGEVVDCVLLYEDRAGIKRFAQRDPLR
                     FAGGEAAAARCGPSLLADLQTGLPIFRGS"
BASE COUNT          285 a          564 c          585 g          282 t
ORIGIN      
        1 acgaacggcg acaggcgatc gtacgtgttg gtcccgttgt tcgccggatc gagctcatcg
       61 cgaaacggga tgcgcacgta atcctgccgg accgccgccg tcgccgagag ctttggcgtc
      121 aagtccacta cggcctgcgc gtatgcggcc gcgtcatctt cgtgcacgac cgcaagcgtc
      181 gcgaccgaat ccgacgtccc gccgccggcg ttcgtcaggc gaaatcgcac gttctgtcgc
      241 gcgtattccg cgccgatcgt gaacgcggtc ggcagggatg cgagcagcaa cggtcgcgtc
      301 cactctgccg tgccgccgcc ggacttgttg tcgatgaatg cgtcggtact gggcggcggc
      361 acgttgacgt tgaattgctc gatgtcgttc cgccgaaaga agagcgtgcc gcgcagcgtg
      421 ccgccccatg cgccccgaaa tccccggagc gcaacgtgga tcaagtcggg cttgaagaaa
      481 tcgcccggcg tgtaattgag ccgcggattc gattggatcc acgtctcggg cagcgaaccc
      541 gcttccgaga tccggttgtt ggcgtacagc acgctgagcg cgatgtcgct ggtgtccggg
      601 ccgcccgacg acgatcgatg cccaacggtc gcgaacaatt ggcgcgtccg cgcgccggtc
      661 gcctgtctcc agccatcttc gttggaggcc gagcccatga tgaagccgtc gatgccgtgg
      721 ctcatgccgc ctgccgagat cgtgccgacc tgctcgccga aggacccggt cgtcagctcg
      781 atgttggccg acggcgtggg cattccccgt tgcgtgaaaa acaacagggc gccgcctaac
      841 gaattgcgcc cgaacaacgc ctgtgatccg cgcacgagct cggcgtggtc gatggcggcc
      901 atcgggagca aatcgaagtt gatctcctgg gcatccggct cgttgacgcg aacgccgtcg
      961 aggaagacgc tcacgccttg gggcgtgccg acgatcggag agatctgaaa gccgcgcacc
     1021 tcgagctcgg gctggagccg cgtcccctga tcgtcgaaga aggtcacgcc gggcagttgt
     1081 tgaagcgagc ggaacgccgc gtccgggccc tgcgagatgt gttgcaagtc catggactcg
     1141 acctgcgacg gaatgcgctc gtcgacgtcg gtgaggcgcg tgccggtcac gaccactttc
     1201 gccaggcgcc gagccgtggt gtcggcccgg gtcgtgtccc gtctggtggt atcgggctgt
     1261 tgggcggcag cggtgatgtg cctaacgcaa agcgcgagaa gcagtgcgac gacgctcctc
     1321 atgagccgcg aaagatcggg agcccggtct ggagatcggc gagaagcgac ggcccgcatc
     1381 tcgccgccgc ggcctcgcca ccggcaaacc ggagcgggtc gcgctgcgcg aaccgcttga
     1441 ttccggcccg gtcctcgtac aacagcacgc aatcgacgac ttcgccggcg cggctcaccg
     1501 tgagcatgcc gtcgtccgcg cgctcgccgc cgtcgagatt gaagcagcac acgtcgtgca
     1561 tgcgatagac gcccgacgcg ctcgcgtgcg tcgttggcct tgcgcccatc cagagggcga
     1621 tcgcggccgc cgatgcgacc agtgtcgcga gtgcgagacc catgccgtgc cggtgtcgca
     1681 ccgagcgcga cggcgcggcc gggacgaacg agcgca
//
LOCUS       DASUCJ010000820         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1063298, whole genome shotgun sequence.
ACCESSION   DASUCJ010000820 DASUCJ010000000
VERSION     DASUCJ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063298"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..992)
                     /locus_tag="VFU90_09795"
     CDS             complement(141..992)
                     /locus_tag="VFU90_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEU5480113.1"
                     /translation="MVGAITPPSAREAAVEEIELQLLLEAVARAAGIDYREYDPNTLR
                     RRVSDRMRAEQVATISALQERVLHDKGALDQFVAAMAGGHRELFYDPAFFMAFRLHVV
                     PLLQTYSFVRLWLPEAGAGEDAYGLAALLDEVGLLSRCIIYATSFSEVSSELARRGEY
                     AIDVLRAVRSAIRNAGFKHPLESFATLENGRVRFAGRLRDGIMFASCDPSSTASINEF
                     HAIVARNVFPLFSGAGQYRLHRVTYQSLARLGFLCLGGGESVAGTVHERAYRQVTPDH
                     NIYRRMR"
     gene            complement(977..>1691)
                     /locus_tag="VFU90_09800"
     CDS             complement(977..>1691)
                     /locus_tag="VFU90_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5480114.1"
                     /translation="AVASAKAALEALAAQEYDCMIVDLGLPDVSGEELIETVRREERF
                     AQLPAIVYTARTLTRDEETRLRELSSTVIVKDGRAPERLLDELRFFLNRVESELPAYK
                     RSRPADTATQTLAGCKALIVDDDSRNIAALQVTLEGHGVIVRTAESGLRAIELLQREP
                     EMDIILMDIMMPEMDGYETIRRIRADDAVKHIPIVALTAKAMKGDRDACIAAGASEYV
                     SKPVDVDQLISLLRIWLAR"
BASE COUNT          282 a          548 c          558 g          303 t
ORIGIN      
        1 tcgtggtgct cttcgtactc tggctgctgg ggtttgcgct acacttcggc ggcggactga
       61 tccacctgtt gctcgtcatc gcgttgatcc tgatcgtttg gaacctgctg atgggtcgcg
      121 gagcgcgcgt ctagcttacc ctagcgcatg cggcggtaga tgttgtggtc gggggtcacc
      181 tggcgataag cccgttcgtg caccgtcccg gccacagact cgccgccgcc gagacagaga
      241 aacccaagcc gcgcgaggct ttggtaggta acgcggtgga ggcgatactg ccccgccccg
      301 ctgaacagcg ggaacacgtt gcgggcgacg atcgcgtgaa actcgttgat cgaagcggtc
      361 gaactgggat cgcacgacgc gaacatgatg ccgtcgcgca gacggccggc gaagcgcacg
      421 cggccgtttt caagggtcgc gaagctttcc agagggtgtt tgaaaccggc atttcggatc
      481 gccgagcgaa cggcgcggag cacgtcgatc gcgtactctc cgcgacgtgc gagctccgac
      541 gagacctccg aaaaggaggt ggcgtaaatg atgcagcgcg acagcaggcc gacctcgtcg
      601 agcagcgccg ccaacccgta ggcgtcctcg ccggcgccgg cttccggcag ccacaaccgg
      661 acgaacgagt aggtctgcag cagcggaacc acgtgcagac ggaacgccat gaagaaggcc
      721 ggatcgtaga acagctcgcg atgcccgccc gccatcgccg cgacgaactg gtcgagcgcg
      781 cctttgtcgt gcagcacgcg ctcctgcagc gccgagatcg tggcaacctg ctcggcgcgc
      841 atccggtcgg aaacgcgccg tcgcagcgtg ttgggatcgt attcgcggta gtcgatcccg
      901 gcggcgcgcg ccaccgcttc gagcagcagc tgcagctcga tctcttcgac cgcggcctcg
      961 cgcgccgaag gcggcgttat cgcgccaacc atatcctcaa cagcgagatc agctggtcga
     1021 cgtcgaccgg tttactgacg tattccgatg cgccggccgc gatgcaggcg tcgcggtcgc
     1081 ccttcatcgc tttggcggta agcgccacga tcggaatgtg cttgaccgcg tcgtcggccc
     1141 gaatccggcg aatggtctcg taaccgtcca tctccggcat catgatgtcc atcaggatga
     1201 tgtccatttc gggctcccgc tgcagcagct cgatcgcccg caggccgctc tctgccgtcc
     1261 gcacgatcac gccgtggcct tcgagcgtta cctgcagcgc cgcgatgttg cggctgtcgt
     1321 cgtcgacgat caacgccttg cagccggcga gcgtctgggt cgccgtgtcg gccgggcggc
     1381 tgcgcttgta agccggcagc tcggattcga cgcggttcaa gaagaagcgc agctcgtcca
     1441 acaggcgttc gggagcgcgt ccatccttga cgatcactgt cgacgaaagt tcccgtagac
     1501 gggtttcctc gtcgcgcgtg agcgtgcgtg ccgtgtacac gatcgcggga agctgtgcga
     1561 accgctcttc gcggcgcacc gtttcgatca gctcctcgcc ggagacgtcc ggaagcccga
     1621 gatcgacgat catgcagtcg tactcttggg cggcgagcgc ctcgagggcg gctttcgcgc
     1681 tggcgacggc g
//
LOCUS       DASUCJ010000821         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_497358, whole genome shotgun sequence.
ACCESSION   DASUCJ010000821 DASUCJ010000000
VERSION     DASUCJ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497358"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>1037)
                     /locus_tag="VFU90_09805"
     CDS             complement(135..>1037)
                     /locus_tag="VFU90_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5480115.1"
                     /translation="NFCRHRGSRLLCGDGVVRGAIRCPYHGWTYSLDGRLIASPFVDS
                     AEVPPESRRLHHVALACWGGFAFLHLSPERVLAGDTLEAQLGAIPARLARYPLAALRT
                     ARSIRYEVGANWKVLLENYNECYHCAGVHPELCRVVPAFKQRGGAGLDWDRGIPHREG
                     AWTFSWSGGSDRAPFPTLDEDERVRHKGELIYPNFMLSLAADHAAAFALWPEGPDRTT
                     VVCDFLFHPDEIAKPGFDPEDAVGFWDLVNKQDWAICESVQAGMRSRAFRYGYYAPME
                     DASLDIRRYVAAHLGEDAVSVDDP"
BASE COUNT          194 a          321 c          360 g          162 t
ORIGIN      
        1 ttcgggcaaa cctggatggt ttcttcaaaa cttcgttctc ccgaaaatcg agtcgagcga
       61 gcggagatac atggacgttc ggagcgagcg tcccggaacc ggagcgttac cgtgaaacga
      121 gcgtaaccgc aaagtcacgg atcgtcgacg gagacggcgt cttcgcctaa gtgcgcggcg
      181 acgtaccggc ggatgtcgag gctggcgtct tccatgggcg cgtagtaacc gtagcggaac
      241 gcgcgcgagc gcatcccggc ttgtacgctt tcgcaaatcg cccaatcttg cttgttgacc
      301 aagtcccaga aaccgacggc gtcctccgga tcgaagccgg gtttggcgat ttcgtcggga
      361 tgaaacagaa agtcgcagac gaccgtcgtg cggtcgggtc cttccggcca cagcgcgaag
      421 gcggcggcgt gatcggcggc gaggctgagc atgaagttcg gatagatcag ctcgcccttg
      481 tggcggacgc gctcgtcttc gtcgagcgtg ggaaacggcg cgcgatcgct gccgcccgac
      541 cagctgaacg tccaggcgcc ttcgcggtgc ggaatcccgc ggtcccaatc gagcccggcg
      601 ccgccgcgct gcttgaacgc cgggacgacg cggcacagtt cggggtgcac gccggcgcaa
      661 tgatagcact cgttgtagtt ttcgagcagc actttccagt tggcgccgac ttcgtaccgg
      721 atcgagcgcg cggtgcgcag cgccgccagc ggatagcgcg ccagccgcgc gggaatcgcg
      781 ccgagttgcg cttcgagcgt atcgccggcg agcacgcgtt cgggcgagag atggagaaag
      841 gcaaaaccgc cccagcaagc gagcgccacg tgatgaaggc gccgcgactc gggcgggacc
      901 tccgccgaat cgacgaacgg cgaggcgatc aacctgccgt caagtgagta ggtccagccg
      961 tggtacgggc agcggatcgc gccgcgcacg acgccgtcgc cgcacaagag ccggctgccg
     1021 cggtggcggc agaagtt
//
LOCUS       DASUCJ010000822         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1307621, whole genome shotgun sequence.
ACCESSION   DASUCJ010000822 DASUCJ010000000
VERSION     DASUCJ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307621"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFU90_09810"
     CDS             complement(<1..568)
                     /locus_tag="VFU90_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5480116.1"
                     /translation="MTEYDAGQIQVLKGLEAVRKRPGMYIGSTSSRGLHHLVYEVVDN
                     SIDETLAGYADTIDVTIHADNAITVVDNGRGIPVDIHPTEKIPGVELAMTVLHAGGKF
                     DKNTYKVSGGLHGVGVSVVNALSGQLKVWVKREGQEYYMDFARGITTTKLKTLRKVPK
                     GERGTTVWFKPDDTIFTELAFDYATLATR"
     gene            843..2315
                     /locus_tag="VFU90_09815"
     CDS             843..2315
                     /locus_tag="VFU90_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5480117.1"
                     /translation="MRRPSGAVNAFLNGVVAALCALVLPLHRPSAQQTGASTTATVEG
                     VVYDSVRWAPLTGAVVQLVSRDEPSQIFTAETDSSGAFSIAGVPRGQFVIGFLHAELS
                     ELDLQAISRNVRVGSKPVVHVALSIPGAVAIRTVLCGAPAAGDSSGTVVGFVRDADSD
                     MPVAGARVTVAWRNFDIDTKGLHSEPRQVDARADASGWFAICGVPGDGIFDIRAQLGA
                     RATPFVQVNAASRGLAIRDLSLGGDSAAAAQPQAAGTSLGRPGAEATAVLEGVIRDPS
                     GTPLAGAEVTLPAAGAETSTGADGRFRIASLPSGTQPLVIAHVGLMPVRTAVDLASHH
                     PASIDIMMTEPVPVLATVKVQGKDVLSKLAGFEQRRTEGFGQFFTAADIAMMEATKLT
                     DVFRRAATLRVIRIDPKDSTFMLGIVSARGQVSVLMANIGFCYPAVYIDGTFMHDAAH
                     NINAFLDPGSVAGIEIYSDASTTPPQYKSGDCGTVLIWTH"
BASE COUNT          418 a          837 c          808 g          453 t
ORIGIN      
        1 ggcgcgtcgc gagcgtcgca tagtcgaacg cgagctcggt gaaaatcgtg tcgtccggct
       61 tgaaccaaac cgtggtgccg cgttcgccct tcggcacttt gcgcaatgtc ttgagcttcg
      121 tggtcgtgat gccgcgcgcg aagtccatgt aatactcctg gccctcccgc ttcacccaca
      181 ccttgagctg ccccgacagc gcattcacca ccgacacgcc cacgccgtgc agtccgcccg
      241 aaaccttgta tgtgttcttg tcgaacttgc cgccggcgtg cagcacggtc atcgcgagct
      301 cgacgccggg aattttttcg gtcgggtgaa tgtcgaccgg aataccgcgc ccgttgtcga
      361 cgacagtgat cgcattgtcg gcgtgaatcg tgacgtcgat cgtgtcggcg tagccagcca
      421 gcgtttcatc gatggagttg tcgacgacct cataaaccag gtggtgcaac ccacgggacg
      481 acgtggagcc gatgtacatg cccgggcgtt tgcgaaccgc ctcgaggcct ttgaggactt
      541 gaatttgacc agcgtcgtat tccgtcatcg atgctcgagc tccttcgcgg gttgaccttt
      601 gccgggcctg cgcgcaccac caaatagaca ccgaagtacg cttcgcgccc aaccatgaaa
      661 agatagctct aaatatttgt taaatcaagt agttaggtgg ctccgtccga ccccctgcca
      721 cgcgtcctca aacgctcgcc acagaactat tgcccggccc ggctttcgtc gttaggctga
      781 gccctgactc gcgacgccgc gcggtgcttc aggtcttcgt cggcagactc gggagggggt
      841 gtatgagacg cccgtcgggt gcagtgaacg ccttcctcaa cggcgtcgtg gccgcgcttt
      901 gcgctctcgt cctgcctctc catcggccca gcgcacaaca gacaggcgca tcgaccaccg
      961 caacagtcga gggcgtcgtg tacgacagcg tgcgctgggc gccgctcacc ggcgccgtcg
     1021 tgcaactcgt gtcgcgcgat gagccgtcgc agatattcac ggccgaaacc gattcctccg
     1081 gtgcgttcag catcgccggc gtgccgcgtg gacaattcgt catcggcttc ctgcacgccg
     1141 agctgagtga gctcgatctc caggccatct cgcgcaatgt gcgggtgggt tccaagccgg
     1201 tcgtgcacgt ggcgctgtcc attccgggag cggtggcgat ccgcaccgtg ctctgcggcg
     1261 cgccggctgc cggcgattcc agcgggacag tcgttggatt cgtgcgcgat gccgacagcg
     1321 acatgccggt cgcgggcgca cgcgtgacgg tcgcgtggcg caatttcgac atcgacacga
     1381 agggactcca cagcgaaccg cgccaggtcg acgccagagc cgacgccagc ggctggtttg
     1441 ccatctgcgg cgtgccgggc gacggcatct tcgatattcg tgcgcagtta ggcgcgcgcg
     1501 ccacgccgtt cgtgcaagtg aacgcggcct cgcgcggact ggccatccgc gacttgagcc
     1561 tgggcggcga cagcgccgcg gccgcgcagc cgcaggccgc gggcacgtcg ttaggcagac
     1621 ccggggccga ggcgacggcc gtgctcgaag gcgtgattcg ggatccgtcg ggcactccgc
     1681 ttgccggcgc ggaagtcacg ctgcccgcgg ccggcgccga aaccagcact ggcgccgatg
     1741 ggcggttccg gatcgcatcg ctcccctcgg gcacgcagcc gctcgtgatc gcgcacgtgg
     1801 ggctcatgcc ggtccgcacc gcggtcgacc tcgcgagcca tcatcccgcc tcgatcgata
     1861 tcatgatgac tgaaccagtg ccggtgttgg cgacggtcaa ggtgcaaggc aaggacgtgc
     1921 tctcgaagct cgccgggttc gagcagcgcc gaacggaagg gttcggccag ttcttcaccg
     1981 ccgccgacat tgcgatgatg gaggcaacga agctgaccga cgtgttccgg cgcgcagcca
     2041 cccttcgcgt gattcgcatc gatcccaagg actcgacctt catgctcggc atcgtgagcg
     2101 cgcggggcca ggtgagcgtc ctgatggcga acatcggatt ctgctacccc gccgtgtaca
     2161 tcgacgggac attcatgcac gacgccgcgc acaacatcaa cgcgtttctg gatcccggct
     2221 cggtcgcagg catcgagatc tactcggacg cgtcgaccac gccgccccag tacaagtccg
     2281 gggactgcgg cacggtgctg atctggacgc actgaccaga acactccgtt aggcgtggag
     2341 gcacgcgcga aacagaacgg gcgctccggc cggctcatcg ccgggggcgc ccgttcgtgg
     2401 ttcgtccgga gtcgacggct actgccggca ggccccgttc ggatcgggct tcgggtggtc
     2461 caccacgacg cgatgatcca ggttcgcgac gtgcagcgcc acgtccgcga cgaagt
//
LOCUS       DASUCJ010000823         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_39149, whole genome shotgun sequence.
ACCESSION   DASUCJ010000823 DASUCJ010000000
VERSION     DASUCJ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39149"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFU90_09820"
     CDS             <1..1486
                     /locus_tag="VFU90_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5480118.1"
                     /translation="RAAKRAGDEGIARRVTFTTAIPQHSSAAIAIEAVPEDFGIKRDV
                     FVKLAAALEPEALLATNTSSLSVGELAAAVDRPERVVGLHFFNPPAAMQLVEVVHTPE
                     TGDDALARAYAFVERIGKVAVLASDSPGFIVNRVARPYYLQAMRALERGVASIEELDA
                     LARSAGFRMGPFELMDFIGIDVNLATSESVYARTDAARLMPLDVQREMVAGGLLGRKS
                     GAGFYDYRNGDPPRLDVSAAPPGGAPNDEEFVAVIGFGGLADELAELLEQRYAHVQRI
                     ENDELLDELSLDTTMAIDVGDGIGDRGEVLAELDSLLGPETVFFTDAYATDVSACAKR
                     MKHPERLIGYGVLGSFAAQSAVEIVDSEAVSDDALELAQEFFGSLGKGVVLVEDVPGL
                     FLGRTVGSIVNEAVTAVEEDVAAPDDVDTAMRLGTNYPIGPIAWGREIGGRRVARILT
                     RLADAEGSAFAPHRALWVLDVEEEGATEPVETGADSAVTGLRGL"
     gene            1486..2691
                     /locus_tag="VFU90_09825"
     CDS             1486..2691
                     /locus_tag="VFU90_09825"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5480119.1"
                     /translation="MPGREVWVVDAVRTPIGRLGGALASVRPDDLAAAALRAVVDRTG
                     VPHDRIDDVYFGDANGSGEDNRNVARMAVLLAGLPVEIPGVTLNRLCGSSLQAINSAA
                     QAIAFGECDAVVAGGVESMTRAPYVLPKSSAPYDRALKLYDTVLGWRMINPRMPAEWT
                     ISLGETAENVAERYGITRAEQDAFALRSQQRCKTAMERGAFEAELVPVRVAATKASPA
                     RDVTVDEHPRPDTTIEALSALKPSFKTGGTVTAGNSSGINDGAAAALLCEASAARALG
                     LKPMARVVACASAGVAPDVMGLGPIPAGRKALALAGLRAADLDLVELNEAFASQSLAC
                     MRDLELDPDKTNVNGGAIALGHPLGASGARLVATLLHELRRRGGRFGMATMCVGVGQG
                     IATIFERTE"
     gene            2706..4202
                     /locus_tag="VFU90_09830"
     CDS             2706..4202
                     /locus_tag="VFU90_09830"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5480120.1"
                     /translation="MSTSTVPATKTQLLIGGAWRDASDGGAYHDDNPATGARLADVAD
                     ATHADLDAAVRAARKAFDGKWPTMAASRRAKIVYKLAQLVGERAKELMLLEVRDNGKA
                     VSTAKGEMGAIVDCFEFYAGAATKNYGVSMPPPLPTYLASTVREPVGVVGAIVPWNFP
                     LLLAAWKVAPALATGCTIVLKPAPSTPLTAIELGKLALEAGVPEGVLNVVTGSGRDLG
                     AWLVEHPGVDKIAFTGSTQTGKAVAASAAQTLKRVTLELGGKSPSVVFDDAHLDGAVA
                     GALYGIYYNAGQACDARSRILLQAGIYDAFVAAFVEKSRKLKVGNPEDPATQVGAITM
                     REQYEKIKAYCELGKGEGAEVLLGGGRAEELDETFDEGLFWSPTAFAAQSDHRIAREE
                     IFGPVATFVRFETEAEAIALANDSDYGLSASVWSRDIGRANRVARAIRSGTVAINTPY
                     AIFPGIPFGGYKQSGYGRELGMETMQAYCETKSVLTYIGEKPMDPFGV"
     gene            4211..4537
                     /locus_tag="VFU90_09835"
     CDS             4211..4537
                     /locus_tag="VFU90_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480121.1"
                     /translation="MADSIKIGQHYDATCGTLWKDGTYFPRGTRVQVVGMHVEQGFCV
                     RFPAELDGEPLETWEDWSETDFLADEGSIEPPNRPLSTAVLAEARDVLSDADDGCHLH
                     LHDEEE"
     gene            complement(4557..4952)
                     /locus_tag="VFU90_09840"
     CDS             complement(4557..4952)
                     /locus_tag="VFU90_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480122.1"
                     /translation="MPKRVSILLAALALGLAIPACNNNSSVTPTPAPSVSLSPNPKIS
                     TATVTVTIQQTPAADIPVQESTPRSSSSPRPGTPFVTQRTNKKGTTKFKNLKPGKTYC
                     WVAILGPHQTSSTCAGWEVWQFQPILLGT"
     gene            complement(5005..>5210)
                     /locus_tag="VFU90_09845"
     CDS             complement(5005..>5210)
                     /locus_tag="VFU90_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEU5480123.1"
                     /translation="SDEEAFVERLWLVHDYRKFAYVDPGLPSELVPAHWPGTRAAALF
                     RTYYDALDRKAQRYFDGCARAGA"
BASE COUNT          891 a         1664 c         1835 g          820 t
ORIGIN      
        1 gcgcgccgcc aagcgcgcgg gagatgaggg gatcgcgcgg cgggttacgt ttaccacggc
       61 gatcccgcag catagcagcg ccgcgatcgc gatcgaggcc gttcccgagg atttcggaat
      121 caaacgcgac gtcttcgtga agctggcggc agcgctggaa ccggaggcgc tgctcgccac
      181 caacacgtcg tcgctttcgg tcggcgaact ggccgcggcg gtcgatcgcc ccgagcgagt
      241 cgtagggctg catttcttca atccgccggc ggcgatgcag ctggtcgagg tcgttcatac
      301 gccggagacc ggcgacgacg cgctggcgcg cgcttacgcg ttcgtcgagc ggatcggaaa
      361 agttgcggtg ctcgcctccg attcgccggg attcatcgtc aaccgcgtcg cacggccgta
      421 ctaccttcaa gcgatgcgcg cgctcgaacg cggcgtcgcg tcgatcgagg agctcgacgc
      481 gctggcgcgc tcggccggct ttcgcatggg gccgttcgag ctcatggatt tcatcggcat
      541 cgacgtcaat ctggcgacca gcgagtcggt ctacgcgcgc accgacgccg cacggctgat
      601 gccgctggac gtacagcgcg agatggtcgc gggcggtctg ctcgggcgca agagcggcgc
      661 cggcttttac gactaccgaa acggcgatcc gccgcggctc gacgtctcgg cggcaccgcc
      721 cggcggtgcg cccaacgacg aggagttcgt cgccgtgatc gggttcggcg gcttggccga
      781 cgagctggcc gaattgctcg agcagcgcta cgcgcacgtg cagcggatcg aaaacgacga
      841 gctgctcgac gagctttcgc tcgacaccac gatggcgatc gacgtcggcg acgggatcgg
      901 cgatcgcggc gaagtgctgg ccgagctcga ctcgctgctc gggccggaaa ccgtgttctt
      961 taccgatgcg tacgccaccg acgtgagcgc ctgtgcgaaa cggatgaaac accccgagcg
     1021 gctgatcggg tacggcgttt tgggatcgtt cgcggcgcag agcgccgtcg agatcgtcga
     1081 ttccgaggcc gtctccgacg atgcactcga gctcgcacaa gagtttttcg gatcgctcgg
     1141 caagggcgtc gtcctggtgg aggacgtccc cggtctgttt ctcggccgca cggtcggctc
     1201 gatcgtcaac gaagcggtga cggcggtcga ggaagacgtc gccgcccccg acgacgtaga
     1261 taccgcgatg cgtttgggca ccaactatcc gatcggaccg attgcctggg gccgcgaaat
     1321 cggcgggcgc cgcgtggcgc gcatccttac gcggctggcc gacgccgaag gaagtgcgtt
     1381 cgcgccgcac cgtgcgctct gggtgctcga cgtcgaggaa gaaggcgcaa ccgaaccggt
     1441 cgaaacgggc gccgattccg ccgtaaccgg gttgcgcgga ctctgatgcc gggacgcgag
     1501 gtgtgggtcg tcgacgcggt gcgcacgccg atcgggcgat tgggcggcgc gctcgcgagc
     1561 gttcgccccg acgacctggc cgccgccgcg ctgcgcgcgg tggtcgatcg caccggcgtg
     1621 ccgcacgacc gcatcgacga cgtctacttc ggcgacgcca acggcagcgg cgaagacaat
     1681 cgcaacgtcg cgcgcatggc ggtgttgctg gccggacttc cggtcgagat tccgggcgtg
     1741 acgctcaacc gcttgtgcgg atcgagtttg caggcgatca attcggccgc gcaggcgatc
     1801 gcgttcggcg agtgcgacgc cgtcgtggcc ggcggcgtcg agtcgatgac gcgtgccccg
     1861 tacgtgctgc ccaaaagcag cgcgccctac gatcgcgcac tgaaactcta cgacaccgtg
     1921 ctgggttggc ggatgatcaa tccccggatg ccggcggagt ggacgatttc gctcggcgaa
     1981 accgccgaga acgtcgccga acgatacggc atcacgcgcg ccgaacaaga cgcgttcgcg
     2041 ctgcgctcgc agcagcgctg caaaacggcg atggagcgcg gcgcgttcga ggccgaactc
     2101 gtgccggtgc gcgtcgcggc gaccaaggcg tcgccggcgc gcgacgtcac cgtcgacgaa
     2161 cacccgcgtc ccgatacgac catcgaagcg ctgtccgcgt tgaagccgtc gtttaaaacc
     2221 ggggggacgg tgacggccgg aaactcttcc gggatcaacg acggtgcggc cgccgcgctc
     2281 ctgtgcgagg cctccgccgc ccgcgcgctg gggttgaagc cgatggcgcg cgtggtggcg
     2341 tgcgcctcgg ccggcgtcgc gcccgacgtg atggggctcg gcccgattcc ggccggccgc
     2401 aaggcgctgg cgctggccgg tctgcgggcg gccgacctcg atctcgtcga actgaacgag
     2461 gcattcgcat cgcagtcgtt ggcctgcatg cgcgacctgg agctggaccc ggacaagacg
     2521 aacgtcaacg gcggtgcgat tgcgctcggc cacccgctcg gagcaagcgg cgcgcggctc
     2581 gtcgccacgc tgctgcacga gcttcgccgg cgcggcggcc gattcgggat ggcgacgatg
     2641 tgcgtcgggg taggtcaggg tatcgcaacg atcttcgagc gaacggagta atgagcaaca
     2701 cggttatgag cacttccacg gttccggcta caaaaacgca actgctgatc ggcggagcgt
     2761 ggcgcgacgc aagcgacggc ggagcctacc acgacgacaa tccggctacc ggtgcgcggc
     2821 tggcggacgt cgccgacgcg acgcacgcgg atctcgacgc cgccgtcagg gcggcgcgta
     2881 aagcgttcga cggcaagtgg ccgacgatgg ctgcgtcgcg gcgcgccaag atcgtgtata
     2941 aactcgcgca gctcgtgggc gagcgtgcca aggagctgat gctgctcgaa gtgcgcgaca
     3001 acggcaaggc ggtctcgacg gcaaagggcg agatgggcgc gatcgtcgat tgcttcgaat
     3061 tctacgccgg cgcggcgacg aagaactacg gtgtgtcgat gccgccgccg ctgccgacct
     3121 acctggccag taccgtgcgc gagccggtcg gcgtggtcgg cgcgatcgtg ccgtggaatt
     3181 ttccgctgct gctggcagcc tggaaagtcg cgccggcgct ggcgaccgga tgcacgatcg
     3241 tattgaaacc ggcgccgtcc acgccgctga cggccatcga gctgggaaaa cttgcgctcg
     3301 aagccggcgt ccccgagggc gtgctgaacg tcgtcaccgg ctcgggccgc gatctcggcg
     3361 cgtggctggt ggaacatccc ggcgtcgaca agatcgcgtt caccggctcg acgcaaaccg
     3421 ggaaagccgt cgcggcctcg gccgcgcaaa cgctcaagcg cgtcacgctc gagctgggcg
     3481 gcaaatcgcc gtcggtcgtg ttcgacgacg cgcacctcga cggcgccgta gccggcgcgc
     3541 tgtacggcat ttactacaac gcgggccaag cgtgcgacgc gcgctcgcgg atcctgctgc
     3601 aggcaggcat ttacgacgcg ttcgtcgccg cgttcgtcga gaaatcgcgc aagctcaaag
     3661 tgggaaatcc ggaagatccg gcgacccaag tgggtgcgat cacgatgcgc gagcagtacg
     3721 aaaaaatcaa ggcgtattgc gagctgggca aaggtgaagg cgccgaagtg ctgctgggcg
     3781 gcggccgcgc cgaagagctc gacgaaacgt tcgacgaggg gctcttttgg agcccgacgg
     3841 cgttcgccgc gcagagcgat caccgcatcg cgcgcgagga gatcttcggg ccggtcgcaa
     3901 ccttcgttcg cttcgaaacc gaagccgaag cgatcgcgct ggccaacgac agcgattacg
     3961 gcctgtcggc cagcgtctgg tcgcgcgaca tcggccgcgc caaccgggtg gcgcgcgcga
     4021 tccgcagcgg aaccgtcgcc atcaatacgc cgtacgcgat cttcccggga attccgttcg
     4081 gcggttacaa gcagtccggc tacgggcgcg aactcggaat ggaaacgatg caggcctact
     4141 gcgaaaccaa gagcgtgctc acctatatcg gcgagaagcc gatggaccca ttcggggtat
     4201 aaacgaagcg atggcggact cgatcaaaat cggccagcac tacgacgcta cgtgcggaac
     4261 cttgtggaaa gacggcacgt actttccgcg cggtacgcgc gttcaagtcg tcgggatgca
     4321 cgtagaacaa gggttctgcg tgcgcttccc ggccgagctc gacggcgaac cgctcgagac
     4381 gtgggaagat tggagcgaaa ccgattttct cgccgacgaa ggttcgatcg agccgccgaa
     4441 ccgcccgctc tcgaccgcgg tcttagccga agcgcgcgac gtgctctccg acgccgacga
     4501 cggctgccat ctgcacctgc acgacgaaga agagtaacga gcgcagaccg cgcggtttag
     4561 gtgccgagca ggatcggctg gaactgccaa acttcccagc cggcgcacgt cgacgacgtc
     4621 tggtgcggac cgagaatcgc gacccagcag taggttttgc cgggtttgag attcttgaac
     4681 ttcgtcgtcc cttttttgtt cgtgcgctgc gtgacgaacg gcgtgccggg gcgcgggctc
     4741 gacgaggaac gcggcgtcga ctcttggacc gggatgtcgg cggcgggcgt ctgctggatc
     4801 gtgaccgtaa cggttgccgt cgaaatcttc ggattcgggc tgagcgagac cgacggcgcg
     4861 ggggtcggcg ttacggaact gttgttgttg caggcgggga tggcgagccc gagggcaagt
     4921 gccgccaaga gaatcgagac gcgtttgggc atggccgggt cggttcgacg cctccgggct
     4981 tcgctccgtt tcggcgcccg gccgtcacgc gccggcgcgc gcgcacccgt cgaaataacg
     5041 ctgcgccttg cgatccaggg cgtcgtaata ggtgcgaaag agggccgcgg cgcgcgtgcc
     5101 cggccagtgc gccggcacca gttcgctcgg cagccccgga tcgacgtagg caaatttccg
     5161 gtaatcgtgc actagccaga gccgctccac gaaggcctcc tcgtcgctca
//
LOCUS       DASUCJ010000824         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_421349, whole genome shotgun sequence.
ACCESSION   DASUCJ010000824 DASUCJ010000000
VERSION     DASUCJ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421349"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFU90_09850"
     CDS             complement(<1..199)
                     /locus_tag="VFU90_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480124.1"
                     /translation="MRQARFTIATALLALLALTGCGLTSSPADTLTFKAPAGWRGSPG
                     MMGFMQFWTSPSSRNEVLMLFK"
     gene            complement(252..932)
                     /locus_tag="VFU90_09855"
     CDS             complement(252..932)
                     /locus_tag="VFU90_09855"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5480125.1"
                     /translation="MKSSVSLILALAMGSCSVAPPAYGRYVPARLARARNIALSRPAP
                     SYVDAVAGAMPLRDGPPVGQPELAVARAILHTNPRVAQAYALLLAVETARAARAHDLP
                     PEFLGATLLQESAYDEGALSAAGAVGIAQFMPDTAAGIGIDPFDPFAAIGGAAELLGG
                     YVSAYRSTYAQPYATALAAYNAGPAAVERYAGVPPYPETREYVALIYDRWFRIVAYER
                     VAAPAAKR"
     gene            923..1468
                     /locus_tag="VFU90_09860"
     CDS             923..1468
                     /locus_tag="VFU90_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480126.1"
                     /translation="MTSSGVLLVAAAAVTLVAGDFASTFFYHVPQHAWFRLHLRTHHD
                     RRRTYWDHAVISRDPAVLLDGFLGAVPYLAVAALLTRVSWPGALLGLALGQLHVWWRH
                     TTDLGWRTPAWLRTALRPLCVVLPEDHDGHHRNPDIEFGDIFRFYDAPARALLARLAP
                     TSRRTRNATGRRNPEKVPVRA"
BASE COUNT          243 a          526 c          515 g          247 t
ORIGIN      
        1 acttaaagag catgaggact tcgttgcggc tgctcggcga ggtccagaac tgcatgaacc
       61 ccatcattcc gggcgagccc cgccaccccg ccggcgcttt gaacgtcaga gtatcggccg
      121 ggctgctcgt caagccgcag ccggtcagcg cgagcagggc caacagcgcc gttgcgatcg
      181 taaagcgcgc ctgtctcatc gaagatcctc ctgagttgcg ccgtaccgtt caactcgaag
      241 caggcggtcg cctatcgctt agcggccggg gccgcgacgc gttcgtacgc gacgatccgg
      301 aaccagcggt cgtaaataag cgcaacgtac tcgcgcgtct ccgggtaggg cgggacgccg
      361 gcatagcgct cgaccgccgc cggcccggcg ttgtaggcgg ccagcgccgt cgcgtacggt
      421 tgtgcgtacg tcgagcgata ggcgctcacg tagccgccca ggagttcggc ggcgccgccg
      481 atcgccgcaa acggatcgaa cgggtcgatt ccgattccgg ccgcggtgtc gggcatgaac
      541 tgcgcgattc cgacggcgcc ggccgccgac agcgcacctt cgtcgtacgc cgattcttga
      601 agcagggtcg cgcccaaaaa ttccggcggc agatcgtgcg cgcgcgcagc ccgcgccgtt
      661 tcgaccgcaa gcaacagcgc gtacgcttga gcgacccggg ggttggtgtg cagaatcgcg
      721 cgcgcgacgg cgagctccgg ctgcccgacc ggcggaccgt cgcgcagcgg catcgcgccc
      781 gcgacggcgt cgacgtacga cggtgcgggg cggctgagcg ctatgttgcg agcccgcgcg
      841 aggcgcgcag ggacgtagcg cccgtacgcg ggcggcgcaa cgctgcaact acccatcgcg
      901 agcgcgagta tcaacgatac ggatgacttc aagcggtgtt cttctcgtcg cagccgccgc
      961 cgtcaccctc gtcgccggcg acttcgcgtc tacatttttc taccacgtgc cgcagcacgc
     1021 gtggttcagg ctccatttgc gcacgcatca cgaccgccgg cggacgtact gggatcacgc
     1081 cgtgatctcg cgcgatccgg cggtgctgct cgacggtttt ctgggtgcgg tgccgtacct
     1141 cgccgtggcc gcactgctga cgcgcgtctc ctggccgggg gcactcctcg gattggcgct
     1201 gggacagctg cacgtctggt ggcgccacac gacggatctc ggctggcgga cccccgcgtg
     1261 gctgcggacg gccctccgtc cgctttgcgt cgtactgccc gaggaccacg acggccacca
     1321 ccgaaacccc gatatcgagt ttggcgacat ctttcgcttt tacgacgccc cggctcgggc
     1381 acttttggcc agactagctc cgacgagccg acggacgcga aacgccaccg gacgccgcaa
     1441 tccggagaag gtaccggtgc gggcctagcc ggcgggggtc tttccgcgac ctgctaaagg
     1501 gagagcgaat gacgaagcgg gccatgttcc t
//
LOCUS       DASUCJ010000825         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_360291, whole genome shotgun sequence.
ACCESSION   DASUCJ010000825 DASUCJ010000000
VERSION     DASUCJ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360291"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFU90_09865"
     CDS             <1..699
                     /locus_tag="VFU90_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5480127.1"
                     /translation="IGRAVAVLFAREGADVAIVFLSEDEDARRTREAVEHEGRECLLI
                     RGDVKDPVFCSQAVDRTLGKFGHLDVLVNNAAFQEHSENIEDLSEEHLQETLQTNIGG
                     YFHMARAAVPHLGEGSAIINTGSETGLFGNPMLLDYSATKGAIHAFTRSLAKNLLKRG
                     IRVNAVAPGPVWTPLNPADRPAGKLDEFGQGSAMGRAAQPEELSPAYVFLAAPSCSSY
                     ISGSVLEVLGGPTG"
     gene            696..1013
                     /locus_tag="VFU90_09870"
     CDS             696..1013
                     /locus_tag="VFU90_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480128.1"
                     /translation="MNTMMMMTDGDLCTLRFRFDGDADRVGTLVSMVRSMENIDRVVE
                     VGTDVPHLRDDSSSAGLSDDRGDFHDVEVHAVDRDAAELARDLVEVAARDLGVVVEYV
                     DRF"
BASE COUNT          179 a          326 c          356 g          173 t
ORIGIN      
        1 atcgggcgtg ccgtcgcggt gctgtttgcc cgggaaggcg cggacgtcgc gatcgtgttc
       61 ctcagcgagg acgaagatgc gcgccgcacg cgcgaagcgg tcgagcacga gggccgcgaa
      121 tgcctgctca tccggggcga cgtgaaggac ccggtcttct gctcgcaagc ggtcgatcgc
      181 accctcggca agttcggcca cctcgacgtc ctcgtcaaca acgccgcgtt ccaggaacac
      241 agcgagaaca tcgaggacct gagcgaggaa cacctccagg aaacgctgca gaccaacatc
      301 ggcggctatt tccacatggc gcgtgcggcg gtgccgcacc tgggggaagg cagcgcgatc
      361 atcaacaccg gttcggagac cggcctgttc ggaaatccga tgctgctcga ttattcggcg
      421 accaagggcg cgatccacgc cttcacgcgc tcgctcgcaa agaatttgct gaagcgcggc
      481 atccgggtga acgccgtggc gcccgggccg gtctggacgc cgctcaatcc cgccgatcgc
      541 ccggccggca agcttgatga gttcggccaa ggcagcgcga tgggccgggc cgcgcaaccc
      601 gaggagcttt cgccggcgta tgtattcctg gccgcgccgt cgtgctcgtc gtacatcagc
      661 ggcagcgtgc tggaagtgtt gggtggtccc acaggatgaa taccatgatg atgatgaccg
      721 atggagatct ttgcacgctg cgcttccgct tcgacggcga cgccgaccgg gtggggaccc
      781 tggtttcgat ggtgcgttcg atggagaaca tcgatcgcgt cgtcgaagtg ggaaccgatg
      841 tcccgcacct gcgcgacgac tcgagctcgg cagggctgtc cgacgaccgc ggggacttcc
      901 acgacgtcga agtccatgcg gtcgatcgtg acgccgccga gcttgcccgc gacctggtcg
      961 aggtcgcggc gcgcgatctc ggggtggtgg tcgagtacgt cgaccgcttc tgagcgatcg
     1021 cggatcgcag cgga
//
LOCUS       DASUCJ010000826         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1063359, whole genome shotgun sequence.
ACCESSION   DASUCJ010000826 DASUCJ010000000
VERSION     DASUCJ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063359"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFU90_09875"
     CDS             complement(<1..404)
                     /locus_tag="VFU90_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480129.1"
                     /translation="MLDAYRGEVFGAALFGALADAQPDEERRERLKTLQTVEARTATS
                     LRRLLANRALHAGDPKVQQDQARALAEKIEPENWHEFLQGLKDALPEFLAKFERTREI
                     APNPSDPALKALVNHERAIERFAELELAGDSTG"
     gene            complement(466..>1742)
                     /locus_tag="VFU90_09880"
     CDS             complement(466..>1742)
                     /locus_tag="VFU90_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480130.1"
                     /translation="FRPVCVTRSLARMADTIRLHWRKSETHGLEPDPEPREIRHTLFS
                     VDDHLVEPPDMFDDRLPAALQADAPRVVETEEGHEVWHFDGKVFFQVGLNAVVGRKRE
                     DWKVEPTRFDEMRPGCYDIDARIRDMDINGVWASVNFPSQITGFCGSVFSRCSDPDLG
                     LAVTRAWNDWFYDVWYTAYPERIVPMGITYLADAEKAAEEIRRNAARGFTAVTLPEMP
                     HRIGMEPIFSSWWDPIIAACAETETVICLHVGSSGVSDIIPGSPVVPLAATLFGQLSL
                     GACAEWLWSGYAVKHPNLKIIMSEGGIGWVAMLHDRLENIVDRSGYGEYFPGNLRPAE
                     VLHRNFWFATIDDPSTLSTRDTIGVDRLTFESDYPHGDGTWPDTQRVFGEVYGGLTED
                     EIAKISHENAAALFRHPLPPPGSPHAVGVRGQ"
BASE COUNT          268 a          583 c          575 g          316 t
ORIGIN      
        1 cccgtgctgt cgcccgccag ttcaagttcg gcgaaccgct cgatcgcgcg ttcgtggttc
       61 acgagcgcct tcagtgcggg atcggatggg ttcggcgcga tctcgcgggt ccgttcgaac
      121 ttggcgagaa actccggcag cgcgtccttg agcccctgga ggaactcgtg ccagttctcg
      181 ggctcgatct tctccgcgag cgcacgcgcc tgatcctgtt gcaccttcgg gtcgccggca
      241 tgtaatgcgc ggtttgcgag caggcgccgc aacgacgtgg cggtgcgcgc ttcgacggtc
      301 tgcaatgtct tcagccgttc ccgccgttcc tcgtcgggct gcgcgtccgc gagcgcaccg
      361 aaaagcgcgg cgccgaatac ctcgccgcga tacgcgtcga gcaacaggtc gtcgtactcg
      421 ttctcactca tggtttgcgc tcctggacct gcgcgtcggc tgtgctcact gccctcgcac
      481 tccgactgcg tgcggacttc cgggcggcgg cagcgggtgc cggaacagtg ccgcggcgtt
      541 ctcatggctg atctttgcga tctcgtcttc ggtgagaccg ccgtagacct cgccgaacac
      601 gcgttgcgta tcgggccagg tgccgtctcc gtgcgggtag tcggattcga acgtgaggcg
      661 atccacgccg atcgtgtcgc gcgtcgacag cgtcgacggg tcgtcgatcg tcgcgaacca
      721 gaagttgcgg tgcagcacct cggcagggcg caggttgccg gggaagtact cgccgtagcc
      781 cgaccggtcg acgatgttct ccaagcggtc gtgcagcatc gcaacccagc cgatcccgcc
      841 ttcgctcatg atgatcttca ggttcggatg cttgaccgcg taccccgacc acaaccactc
      901 cgcgcacgcg ccgagcgaga gctggccgaa cagcgtcgcc gccagcggaa cgaccggcga
      961 accgggaatg atgtcgctga cgcccgacga accgacgtgg agacagatga cggtttcggt
     1021 ttccgcgcac gcggcgatga tcgggtccca ccacgacgag aagatcggct ccattccgat
     1081 gcggtgtggc atctccggca gcgtcaccgc ggtgaacccg cgcgccgcgt tgcggcgaat
     1141 ctcctccgcc gctttctcgg cgtcggcgag ataggtgatg cccatcggga cgatgcgctc
     1201 cgggtatgcg gtgtaccaca cgtcgtagaa ccagtcgttc cacgcgcgcg tcaccgccag
     1261 cccgaggtcg ggatcgctgc agcgcgagaa caccgagccg cagaagcccg tgatctgtga
     1321 cgggaagttc accgacgccc acacgccgtt gatgtccatg tcgcgaatgc gtgcgtcgat
     1381 gtcgtagcag ccgggccgca tctcgtcgaa gcgcgtcggc tcgaccttcc agtcttcgcg
     1441 cttgcgtccg acgaccgcgt tcaacccaac ctggaagaac accttgccgt cgaagtgcca
     1501 cacctcgtgg ccttcctcgg tctcgacgac gcgcggcgca tcggcctgca gtgccgcggg
     1561 caggcggtcg tcgaacatgt ccgggggctc gacgaggtgg tcgtcgaccg agaagagcgt
     1621 gtggcggatc tcacgcggct cgggatccgg ctcgagtccg tgcgtctcgc tcttgcgcca
     1681 gtggaggcgg atcgtgtcgg ccatgcgcgc caggctacgt gtcacacaga cgggccggaa
     1741 gt
//
LOCUS       DASUCJ010000827         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_696341, whole genome shotgun sequence.
ACCESSION   DASUCJ010000827 DASUCJ010000000
VERSION     DASUCJ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696341"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFU90_09885"
     CDS             <1..542
                     /locus_tag="VFU90_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480131.1"
                     /translation="LPWQRFERFAHEYGLNARQATQLVENAPLAVYFDRVVLASKNPQ
                     QSTNFVLGELSRLSNESGVAVTDSAVSPESLAELIALVESKTVNSKIAKELLVRMWGG
                     AGSPKAIVEKEGLAQTSDPGAIAKLVDDVVASNAKVVADYKAGKTNVAGFLVGQIMKA
                     SGGKADPALVRELLQKKLE"
     gene            551..>2751
                     /locus_tag="VFU90_09890"
     CDS             551..>2751
                     /locus_tag="VFU90_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HEU5480132.1"
                     /translation="MLADERTLEALDFAGVRDRVVAATHTQRGRALAVALAPSTDFDG
                     VVRVEQQRTAAARDLVASADLHVTSAIDTAQLTQAASVGRTLGPSDLRAVADALAAAA
                     AAYNAVRSDAVLRDVAGGYVALRDLQRAISDGIDERGIVLDRASPALGRIRRSLAQAQ
                     VEARDRVGAILKSAKYAKAIQDNVVTIREGRFVVPVKAEFSGEFPGIVHDTSSSGQTL
                     FIEPLAALDSNNRLRTLRIEEEREVARILEEFSRRVGADSAAIERNVDILAAIDLLVA
                     KAELARTMDAVAPELSESPSVAIHNGRHPLLGARAVPQSLTLDDATRLLVISGPNMGG
                     KTVALKMVGLFVVMAGCGMQIPAALGTTIGRFERIVADIGDEQSIVANTSTFSAHLQR
                     MRELLDSADGRTLAIVDEIGGGTEPSAGAALAIAMLERLLALGARGVVTTHATELKLF
                     AHGAAGVANASVRFDPQTFRPTFHLDLGSPGQSLAFPLARTIGIDETIVDRAQQLLDN
                     RERDYESALAELSLRNAELQAEREGLASQRRAAESESTAVQRERALLEEERQRFGARA
                     EERLQQSLREFARELQRRADAAAPARPKVSASQAALLARTIDAMHRDLGIRPDEPERG
                     DDGTYEPNERVRVASLKQEGTVVEDYGDTLLIAVGPMKTVVKKSDVRRSEAASAPRKR
                     ARSSSGEARIETVARSTSQLDVRGKRYAEAEPLVERWLDDALLSGASPLRLV"
BASE COUNT          494 a          807 c         1022 g          428 t
ORIGIN      
        1 aactgccgtg gcagcggttc gagcgcttcg cgcacgagta cggattgaat gcgcgccagg
       61 cgacgcaact ggtcgaaaac gcgccgctgg cagtgtattt cgatcgagtg gtgctggcga
      121 gtaagaatcc gcagcagtcg acgaacttcg tgctcggcga gctgtcgcgg ctgtcgaacg
      181 aatcgggggt cgcggttacg gattcggcgg tgtcgcccga gagtctcgcc gaattgattg
      241 ctttggtcga atcgaagacg gtcaattcga agatcgccaa agagctgctc gtgcgaatgt
      301 ggggcggggc gggttctccg aaggcgatcg tcgagaaaga ggggctcgcc cagacgagcg
      361 atccgggggc gatcgcgaaa ctggttgacg acgtcgtcgc ttcgaatgcg aaagtcgttg
      421 ccgactacaa agccggcaag acgaacgtgg cgggattctt ggtggggcag atcatgaagg
      481 cgtcgggcgg aaaggccgat ccggcgctcg tgcgcgagct gctacagaag aagctggagt
      541 aactcgggcg atgctcgccg acgaacgaac gctcgaagca ctcgacttcg cgggcgtgcg
      601 cgaccgcgtg gtcgcggcga cgcatacgca gcgtggccga gcgttggcgg tggccttggc
      661 accatcgacc gatttcgacg gcgtggtgcg ggtcgagcag cagcgcactg cggcggcgcg
      721 agacctggtt gcgagcgccg atttgcacgt aacgtcggcg atcgatacgg cacaactcac
      781 gcaagcggcg tcggtggggc gaacgctcgg gccgagcgac ttgcgcgcgg tcgcggacgc
      841 gctggcggcg gcggcggcgg cgtacaacgc cgtgcgctca gacgcggtgc tgcgcgacgt
      901 ggccggcggg tacgttgcgc tacgcgatct gcagcgtgcg atttcggacg gaatcgacga
      961 acgcggcatc gtgctcgacc gggcatcgcc ggcgctgggc cgcatccggc gcagtctggc
     1021 ccaggcgcaa gtcgaggcgc gcgaccgggt cggcgccatt ctgaaatcgg cgaaatatgc
     1081 gaaggcgatt caagataacg tcgtgaccat tcgcgaggga cggttcgtcg tgcccgtcaa
     1141 ggcggaattt tcgggcgagt ttccggggat cgttcacgac acgagttcga gcgggcagac
     1201 gctcttcatc gaaccgctgg cggcgctcga ttccaacaat cggctgcgaa cgctgcgcat
     1261 cgaggaagaa cgcgaggtgg cgcggattct cgaagagttc tcacggcggg ttggtgccga
     1321 ttcggctgcg atcgaacgca acgtcgacat tctggcggcg atcgatctgc tggttgcgaa
     1381 ggcggagctg gcgcggacga tggacgcggt tgcgcccgag ttgagcgaat cgccgtcggt
     1441 cgcgattcac aacgggcgtc atccgttgct cggtgcgcgc gcggtgccgc aatcgttgac
     1501 gctcgacgac gcgacgagac tgctggtcat cagcggcccg aacatgggcg ggaagaccgt
     1561 cgccctgaaa atggtggggc tgttcgtggt gatggcgggg tgcgggatgc agattccggc
     1621 cgcgctcgga acgacgatcg gccgcttcga gcgcatcgta gcggatatcg gcgacgagca
     1681 gtcgattgtc gcgaacacgt cgacgttctc ggcgcacttg cagcggatgc gggagctgct
     1741 cgacagtgcg gacggtagaa ctcttgcgat cgtcgacgaa atcggcggcg gcaccgagcc
     1801 cagcgcgggt gcggcgctcg cgatcgcgat gctcgaacgg ctgctcgcgc tgggcgcacg
     1861 cggcgtcgtc acgacgcacg ctaccgagct caagctgttc gcccacgggg cggcgggcgt
     1921 cgcaaatgcg agcgtgcgat tcgatccgca gacgttccgt ccgacgtttc atctcgacct
     1981 cgggtcgccg ggccagtcgc tggccttccc gctggcccgc acgatcggca tcgacgagac
     2041 gatcgtagac cgcgcgcagc agctgctcga caatcgcgag cgcgactacg agtcggcgct
     2101 cgcagagctg tcgctgcgta acgccgaatt acaagccgaa cgggagggac tggcgtcgca
     2161 acggcgcgcc gccgaaagcg agagcacggc ggtacagcgc gaacgggctt tgctggagga
     2221 ggagcgccag cgttttggcg cgagggctga agaacgactg cagcagtcac tgcgcgaatt
     2281 cgcccgcgag ctgcaacggc gcgcggacgc agcggcgccg gcgcgcccca aggtgagcgc
     2341 gtcgcaggcg gcgttgctgg cgcggacgat tgacgcgatg catcgcgatc tcggaattcg
     2401 accggacgag ccggagcgcg gcgacgacgg gacgtacgaa ccaaacgaac gcgttcgcgt
     2461 ggcgtcgctg aagcaagagg gcaccgtcgt cgaggactac ggcgacacgc tgctgatcgc
     2521 ggtcggcccg atgaaaacgg tcgtcaagaa aagcgacgtg cgccggagcg aagccgcgag
     2581 cgcgccgcgg aagcgggcgc gttcttcgag cggtgaggcg cggatcgaaa cggtagcgcg
     2641 gagcacgtcg cagctcgacg tgcgcggaaa gcgttacgcc gaggcggagc ccctggtcga
     2701 acggtggctc gacgacgcat tgctgtcggg agcttcaccg ctccggctgg t
//
LOCUS       DASUCJ010000828         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_559076, whole genome shotgun sequence.
ACCESSION   DASUCJ010000828 DASUCJ010000000
VERSION     DASUCJ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559076"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFU90_09895"
     CDS             complement(<1..728)
                     /locus_tag="VFU90_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480133.1"
                     /translation="MSARYGIVSVGTNSTRLLLADVSGDVPRVALARTIGTRIGEGLG
                     ERGHLGEEPMRRTLDAVAQFYREIRGRYVRLFAVATSALRRADNGEEFLQRAGELLGV
                     PVRVLSGEEEARASYRGAITAFGRLRGERAGVVDIGGGSTEFAAGTGVTPERVLSFEI
                     GAVRLTEAVPALSGNAGAVDAGSLDRARTLAREALAPLDGGEPLERLALVGGTATTTA
                     SIVRGRSAPVDAFRLTRDDLAGVLA"
     gene            complement(731..1084)
                     /locus_tag="VFU90_09900"
     CDS             complement(731..1084)
                     /locus_tag="VFU90_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480134.1"
                     /translation="MSETIDGLVINLNVYGATVRLESGELATAPAADVDAHRGDYQKS
                     LVHRKSLVFELRRDGRRPTVLLAPQIHDEELDARIASYLRSTQEWDDPDGVPAADRHF
                     LQKKRRAALFESRHR"
     gene            complement(1081..1401)
                     /locus_tag="VFU90_09905"
     CDS             complement(1081..1401)
                     /locus_tag="VFU90_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480135.1"
                     /translation="MKRRVHARPVIRLLGRVTASALGLLVFTLVGIQFARVIGQDVSM
                     ARDLSSVNAQIAALKKHEIRQRAEIRRLRDPEGAVPEIHDRLRMVRSNEEIIFVSPAP
                     SPAP"
     gene            complement(1398..2384)
                     /locus_tag="VFU90_09910"
     CDS             complement(1398..2384)
                     /locus_tag="VFU90_09910"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5480136.1"
                     /translation="MSNNLVVEREKGLLELERRIAELRAANASQPVDMSSEIAALERK
                     YQDILQEVFGALTPWEKVHMARHPNRPTTLDYYSQFERFDELHGDRQFRDDPAIVAGF
                     ARLRGRSVMAIGQQRGRDTKENLRRNFGMPAPEGYRKVRRLAQVASRLGLPIVTFVDT
                     KGADPGISSEEHAQSEAIAGCLLEFTAARVPIVATVIGEGGSGGALALAIADRVLMLE
                     HSTYSVASPEGCAAILWSDAGKAEEAAARLKLTAQDLQSFGIVDEIVPEPLGGAHRDA
                     AGVVTRVLSSVDAAIARLEALSPEELLEDRYRKYRRLGAWQSERLEPIERSS"
     gene            complement(2381..3223)
                     /gene="accD"
                     /locus_tag="VFU90_09915"
     CDS             complement(2381..3223)
                     /gene="accD"
                     /locus_tag="VFU90_09915"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5480137.1"
                     /translation="MPMPDWLLTRRSKDAQSRDAALWSKCPKCGEVLYRRDLAANLYV
                     CTKCSHLFRMNAYDRINSIVDGDFTEIGGDVTSGDPLGWSDKKTYPAKLSGDREKSGL
                     SEAVVCGFTKIDGFDVGLGVMDFHFRGGTMGTVVGERITRLLEEATRRKVPCILFTAS
                     GGARMEEGMLALVQMAKTTAAVTRFVEGGNFLLVVLTDPTTGGVAASFAFQADVIVAE
                     SKAMIGFAGRRVIEQTIRQKLPDQFQTAEFLLEKGAIDMVVERHALKDTLVRLLQYAA
                     AGRR"
     gene            complement(3230..3943)
                     /locus_tag="VFU90_09920"
     CDS             complement(3230..3943)
                     /locus_tag="VFU90_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU5480138.1"
                     /translation="MALPFSIVIPTYNEAGGIERLLRALCAVFSANSLDGEIIVVDDK
                     SPDGTGAIVDRLADELPVRCLHRPGKLGLSSGVIDGWKLARKESAALGAMDADFSHDV
                     NVIPKMVAALESGYGLAVGSRYVEGGGITNWPPRRIVTSRVACWLARPLTAVKDVTSG
                     FFLVRREALEGVELDPIGFKIGLEVIVKGKYGKAVEIPYVFTDRVVGESKLNQKEIFN
                     YLKQLRKLYSARLLPSHKV"
     gene            complement(3959..>4595)
                     /locus_tag="VFU90_09925"
     CDS             complement(3959..>4595)
                     /locus_tag="VFU90_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU5480139.1"
                     /translation="SFSAAVTIEASAVNATLGEKTAPAKRVADFDVSVAQALPKGQKM
                     DFVVEKTTELGAGAILPFCSERAIAREASAAKLARWRRLAKTAAQQSGRADVPVVAGV
                     SSYAALLDRFAEFDAVLFAWELAARVPLRERLPQLLAGARRVLIVVGPEGGFTHAEAE
                     AASARGAAAIWLGERILRTETAALALLAIVDTFAPYGTETPAGPELSAGPS"
BASE COUNT          748 a         1628 c         1465 g          754 t
ORIGIN      
        1 gcgagcacgc ccgccaagtc gtcccgcgtc agccgaaaag cgtccaccgg cgcgctgcgc
       61 ccgcgcacga tcgaggccgt cgtggtggcc gtaccgccga ccagcgccag ccgttcgagc
      121 ggctcgccgc cgtccaacgg cgcgagcgct tcgcgggcga gggtgcgcgc ccggtcgagc
      181 gaccccgcat cgaccgcgcc ggcgttcccg gagagcgcgg gaacggcctc cgtcagccgc
      241 accgcgccga tttcgaacga cagcacgcgt tcgggcgtga cgcccgtgcc ggctgcgaat
      301 tcggtgctgc cgccgccgat gtcgaccacg ccggcgcgct cgccgcgcag ccgtccgaac
      361 gccgtgattg cgccgcggta cgaagcgcgc gcttcttcct cgcccgagag cacgcgcacc
      421 ggcacgccga gcagctcgcc cgcgcgctgg agaaactcct caccgttgtc cgcgcgccgc
      481 agcgcgctgg tcgcgaccgc gaacagccgc acgtaccgcc cgcgaatctc gcgatagaac
      541 tgtgcgacgg cgtcgagcgt gcgccgcatc ggctcttcgc cgaggtgccc gcgttcgccg
      601 agcccttcgc cgatgcgcgt tccgatcgtg cgcgcgagtg cgacccgcgg cacgtcgccc
      661 gaaacgtcgg ccagcagcag ccgcgtcgaa ttcgtgccga ccgaaacgat gccgtagcgg
      721 gcgctcacac ttaccggtgc cgggattcaa acagcgccgc gcgccgcttt ttctgcagaa
      781 aatgccgatc ggccgcggga acgccgtcgg ggtcgtccca ttcttgcgtg ctgcggagat
      841 acgacgcgat gcgcgcgtcg agttcctcgt cgtggatctg cggcgccaac agcaccgtcg
      901 ggcggcgtcc gtcgcgacgc agctcgaaaa ccagcgattt gcgatgcacc agcgattttt
      961 gatagtcgcc gcgatgggcg tcgacgtcgg ctgccggcgc ggtcgccagc tcgccgcttt
     1021 ccagccggac ggtcgcgccg tagacgttga ggttgatgac caggccgtcg atggtttcgc
     1081 tcacggggcc ggcgacgggg ccgggctcac gaagatgatc tcttcgttcg agcgcaccat
     1141 gcggagccgg tcgtgaattt ccggaacggc gccctcggga tcgcgcagcc gccggatctc
     1201 ggcgcgctgc cggatctcgt gcttcttcaa ggctgcgatc tgagcgttta ccgacgaaag
     1261 gtcgcgggcc atcgagacgt cttggccaat cacgcgcgcg aactggatgc cgacgagtgt
     1321 gaataccaaa aggcccagcg ccgacgccgt gacccgcccg agaagacgaa tgaccgggcg
     1381 agcgtgcacg cgccgcttca actgctccgc tcgatcggct ccaagcgttc gctttgccac
     1441 gcgcctagcc gccggtactt ccgatagcga tcctccaaca gttcttccgg cgagagcgct
     1501 tccaatcgtg cgatcgccgc atccaccgaa ctcaggacgc gcgtcacgac gccggccgcg
     1561 tcgcgatgcg ctcccccgag aggctccgga acgatttcgt cgacgattcc gaaactctgc
     1621 aggtcctgcg ccgttaactt caaccgcgcc gccgcttctt ccgctttgcc cgcatccgac
     1681 cacagaatcg ccgcgcagcc ttcgggcgaa gccaccgagt aggtgctgtg ttcgagcatc
     1741 agcacgcgat cggcgatcgc gagcgcgagc gcgccgccgg agccgccttc gccgatgacg
     1801 gtcgccacga tcggaacgcg tgcggccgta aactcgagca ggcaaccggc gattgcctcg
     1861 gactgcgcgt gctcttcgga gctgatgccg gggtccgcgc ctttggtatc gacaaacgtg
     1921 acgatcggca aacccagccg cgaggccacc tgagcgagcc gccgcacctt gcgatacccc
     1981 tcgggcgccg gcatcccgaa gttgcgccgc aagttttcct tggtatcgcg tcctcgctgc
     2041 tgcccgatcg ccatcaccga gcggccgcgc agccgcgcga agccggcgac gatcgccggg
     2101 tcgtcgcgaa actgccggtc gccgtgcagc tcgtcgaagc gctcgaactg cgagtaatag
     2161 tcgagcgtcg tcggccggtt gggatggcgc gccatgtgca ccttctccca gggcgtgagc
     2221 gcgccgaaga cttcctgaag gatgtcttga tacttgcgtt cgagcgccgc gatctccgac
     2281 gacatatcga ccggctgcga ggcgttggcg gcgcgcagct cggcgatccg gcgctcgagt
     2341 tcgagcaaac ccttttcgcg ctcgacgacc aggttgttgc tcatcggcgc cccgcggcgg
     2401 cgtattggag caggcgcacc agcgtatctt tgagcgcgtg acgctcgacc accatgtcga
     2461 tcgcgccctt ttcgagcaag aactcggcgg tttgaaactg atcgggcagc ttctgacgga
     2521 tcgtctgctc gatcacccgc cggcctgcga aaccgatcat cgctttcgat tccgccacga
     2581 tcacgtcggc ttgaaatgca aacgaggcgg cgacgccgcc ggtggtcgga tcggtgagga
     2641 cgaccagcaa gaaattgccg ccttcgacga agcgcgtcac cgcggcggtc gttttcgcca
     2701 tctgcacgag cgcgagcatc ccctcttcca tgcgcgcgcc gcccgaagcg gtgaatagta
     2761 tgcacggaac cttgcggcgg gtcgcctctt ccagcagccg cgtaatccgc tcgccgacga
     2821 ccgtccccat cgtgccgccg cggaagtgaa agtccatcac cccgagtccg acgtcgaaac
     2881 cgtcgatttt cgtgaaaccg caaaccaccg cttcggagag cccgctcttc tcgcggtcgc
     2941 cgctcagctt ggcgggatac gtcttcttgt cggaccagcc cagcggatcg cccgacgtca
     3001 cgtcgccccc aatttcggta aagtcgccgt cgacgatcga attgatacgg tcgtacgcgt
     3061 tcatccggaa gaggtgactg catttcgtgc agacgtacag attggccgcg agatcgcggc
     3121 ggtagagcac ctcgccgcac ttcgggcatt tgctccacag cgcggcgtcg cgcgactgcg
     3181 cgtctttgct gcggcgggtg agcagccagt ccggcatcgg catcggtacc tacaccttgt
     3241 ggctcggcag caaacgcgcc gagtagagct ttcgcagctg cttgaggtag ttgaagatct
     3301 ctttttgatt gagcttcgac tcgccgacca cgcgatcggt gaagacgtac gggatctcga
     3361 ccgcctttcc gtacttcccc ttgacgatca cttcgaggcc gatcttgaac ccgatcgggt
     3421 cgagctcgac gccctccaac gcttcgcgcc gcaccagaaa gaaaccgctg gttacgtctt
     3481 tgaccgccgt aagcgggcgc gccagccagc acgcgacccg cgacgtgacg attcggcgcg
     3541 gcggccagtt ggtgatcccg ccgccctcga cgtaacggct tccgaccgcc aaaccgtacc
     3601 ccgattcgag cgccgcgacc atcttgggaa tgacgttgac gtcgtgactg aaatcggcat
     3661 ccatcgcgcc gagcgccgcc gattctttgc gcgcgagttt ccatccgtcg atcacgcccg
     3721 acgaaaggcc cagctttccc ggccggtgca ggcagcgaac gggcagctcg tcggccagcc
     3781 ggtcgacgat cgcgccggtg ccgtcgggcg acttatcgtc gacgacgatg atctcgccgt
     3841 cgagtgagtt ggccgaaaag acggcgcaca gcgcgcgcag gagccgctcg atgccgccgg
     3901 cttcgttgta cgtcggaatg acgatggaaa agggtagcgc catagtcaat cggcgtatct
     3961 acgacgggcc tgcgctcagt tccggcccgg ccggcgtctc ggtgccgtac ggcgcgaacg
     4021 tgtcgacgat cgccagcagc gccagcgccg ccgtctcggt ccgcaggatg cgttcgccca
     4081 gccagatcgc cgcggcgccg cgcgccgagg ccgcctcggc ctcggcgtga gtgaagccgc
     4141 cctccgggcc gacgacgatc agcacgcggc gcgcgccggc cagcagttgc ggcagccgct
     4201 cgcgcagcgg cacgcgcgcg gcgagttccc acgcgaacag caccgcgtcg aactccgcaa
     4261 agcgatcgag tagcgccgcg tacgacgaca cgccggccac caccggaacg tccgcgcggc
     4321 ccgattgctg ggctgccgtt ttggccagcc gccgccagcg ggcgagcttg gcggcactcg
     4381 cctcgcgcgc gatcgcgcgc tccgagcaaa aaggaagaat cgcgcccgcc ccgagctcgg
     4441 tcgtcttttc gacgacgaaa tccatcttct gacccttcgg cagcgcctgc gcgacggaca
     4501 cgtcgaaatc ggcgacccgc ttcgccggcg ccgttttttc cccgagcgtc gcgttcaccg
     4561 ccgacgcctc gatcgtcacg gcggcgctaa acgag
//
LOCUS       DASUCJ010000829         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1170345, whole genome shotgun sequence.
ACCESSION   DASUCJ010000829 DASUCJ010000000
VERSION     DASUCJ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170345"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFU90_09930"
     CDS             complement(<1..431)
                     /locus_tag="VFU90_09930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5480140.1"
                     /translation="MAALLLRLPAPPKIVDRKLAGRMHSKARDAAAISFHYDLPLAFY
                     RTFLDAGLVYSCAYWDEGIASLEGAQRAKLDYVLDKLRLQPGMRFLDIGCGWGSLVMR
                     AAQRGAQALGITLSRPQYEEAQRRIAAAGLTANARVELRDYR"
     gene            complement(447..743)
                     /locus_tag="VFU90_09935"
     CDS             complement(447..743)
                     /locus_tag="VFU90_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480141.1"
                     /translation="MAIAARDKTAERAMEVVRLLFGDRLGRDAAVRLWDGTAVLARDP
                     RFELIVATPFALRAAFSPPLNLNPGRAYVEGWIDVAGDLEAAVDAMERCVEGLS"
     gene            complement(747..1442)
                     /locus_tag="VFU90_09940"
     CDS             complement(747..1442)
                     /locus_tag="VFU90_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5480142.1"
                     /translation="MSHTLTPADFVVRLAIGAGLGVLIGFERQWRQRTAGLHTSGLVA
                     TGATLFALLDTVINGNDVSRILAGIVTGVGFISGGVILRDGANVVGLNTAATIWATAA
                     VGALAGVGFFEQAAVAAVAILLLNVLMQPLAERIDYRSRHRKNRETIYKLQIACTAAQ
                     QSAVRHAIVTAVSESPLSLRSLINRNAAAANVEINADLYSPVRNDPLIEVLASELSAM
                     PGVSASDWHAVDV"
     gene            complement(1511..>1827)
                     /locus_tag="VFU90_09945"
     CDS             complement(1511..>1827)
                     /locus_tag="VFU90_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480143.1"
                     /translation="GHRWTRLRLEYETLAEHLGRFDTAYARPWQPGDVAYAGLIAASD
                     PATTPLRSYPFGEGATGRTDAGALADATVALAAGYSAAACIQTNVPRPTPSLVLRPNL
                     DR"
BASE COUNT          298 a          632 c          620 g          277 t
ORIGIN      
        1 cggtagtcgc gcagctcgac ccgcgcgttt gcggtgagcc cggcggcggc gatgcggcgc
       61 tgggcttctt cgtactgcgg ccgcgacagc gtgatcccca gcgcctgcgc gccgcgctgg
      121 gcggcgcgca tgaccagcga accccacccg cagccgatgt cgagaaaccg catgccgggc
      181 tgcagccgga gcttgtcgag cacgtagtcg agcttggcgc gctgcgcgcc ttcaagcgac
      241 gcgatgcctt cgtcccagta ggcgcacgag tacaccagtc ccgcgtcgag aaacgtccgg
      301 tagaacgcca acggcaaatc gtagtggaag ctgatcgcgg ccgcgtcgcg cgctttcgaa
      361 tgcatgcgcc ctgccagttt gcggtctacg atcttgggcg gcgccggcag gcgaagcagc
      421 aacgcggcca tgcgcgcgag gacggcctac gaaaggcctt cgacgcagcg ctccatcgcg
      481 tcgacggccg cttcgaggtc gcccgcaacg tcgatccaac cctcgacgta cgcacgcccc
      541 ggattcaaat tcagcggcgg cgaaaaggcc gcccgcagcg cgaacggcgt cgccacgatc
      601 agttcgaagc gcggatcgcg cgcgagcacc gccgttccgt cccacaagcg caccgcggca
      661 tcgcgaccga gccgatcccc gaacagcagg cgcacgacct ccatcgcgcg ttccgccgtc
      721 ttgtcgcggg ccgcgatcgc catcgctcag acgtccaccg cgtgccagtc ggacgccgag
      781 acgcccggca tcgccgacag ttccgacgcg agaacctcga tcagcgggtc gttgcgcacc
      841 ggcgagtaca agtcggcgtt aatttcgacg ttggcggcgg cggcgttgcg attgatcagc
      901 gaccgcaggc tcagcgggga ttcgcttacg gccgtgacga tcgcgtggcg aacggccgac
      961 tgctgcgcgg ccgtgcacgc gatctgaagt ttgtaaatcg tttcgcggtt cttgcggtgc
     1021 cgcgagcggt agtcgatgcg ctcggccagg ggttgcatca gcacgttcag cagcaggatg
     1081 gcgaccgcgg cgacggcggc ttgttcgaag aacccgacgc ccgccagcgc gccgaccgcg
     1141 gcggtggccc agatcgtggc ggcggtgttg aggccgacga cgttggcgcc gtcgcggaga
     1201 atgacgccgc ccgaaatgaa tcccacgccg gtgacgattc cggccaagat gcgcgagacg
     1261 tcgttcccgt tgatgacggt atcgagcagc gcgaacagcg tcgcgcccgt ggcaacgagt
     1321 ccgctggtgt gcagcccggc cgtgcgctgc cgccactggc gctcgaatcc gatgaggacg
     1381 ccgagccccg caccgattgc cagccggacg acgaaatccg ccggcgttaa cgtgtggctc
     1441 acgcgatccc aagcctttcc ttcacgccgg ttcgtcgttc ggcacgctcg ccgaattctc
     1501 tccgaaccgg ctagcggtcg agattgggac gcaacaccag cgacggcgtg ggccgcggaa
     1561 cgttggtttg aatgcaggcg gccgccgaat agccggccgc cagcgcaacg gtggcatcgg
     1621 ccagcgcccc cgcgtcggtg cggcctgtgg cgccctcacc gaacgggtac gatcgcagcg
     1681 gggtcgtcgc cggatcgctc gctgcgatca gtcctgcgta cgcgacgtcg ccgggttgcc
     1741 acgggcgtgc gtaagccgtg tcgaagcgcc ccaaatgctc tgccagcgtt tcgtattcca
     1801 aacgcaggcg cgtccagcgg tggccct
//
LOCUS       DASUCJ010000830         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_8902, whole genome shotgun sequence.
ACCESSION   DASUCJ010000830 DASUCJ010000000
VERSION     DASUCJ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8902"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..>1342)
                     /locus_tag="VFU90_09950"
     CDS             complement(196..>1342)
                     /locus_tag="VFU90_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU5480144.1"
                     /translation="TLRKSGGAIESHQDTYAALLVSIGSARNGVARLRGFETATEASY
                     FDRSIDPASVERTLRAVRASGAYGRYLGVAAKAPKPAFTPGRFAIPDALALILAAERP
                     MGTDYANAYAELFDPANRRLEICTAPECDDTGFSVGFAGVESGVYFGNFDGETHSLRA
                     VAHESGHAVHREFMARNQPIAAYNRGPSFMFESFAIFNELLFLNYVYQRAATDAQRAY
                     YLNYFLDDATFQVYGSAEETDLESAIYRGIAEGTVKTAADFDALNLKIFARYDPAAGT
                     DPATQLYWARNRLYYTDPLYDVNYLYAGLLALAYFKEFERAPQAFVPRYVALLKHGFD
                     DSPAALERRFLGIDLSDEPALVESAAATIDARTQTLAKLYSSPAEGR"
BASE COUNT          250 a          382 c          456 g          254 t
ORIGIN      
        1 gtgttggcga cgatcgaaca ccggagaaat attcgcacgc tcggagcgca gtacgccggc
       61 cggcggtggt cgctggcggt aattctcgga gcgctgtttt ctctgctggg gatcgtcgcg
      121 ctgctcgcga ttatctttcg aaaatagaac gccgcgcggc gacggccgcc gccgctccgt
      181 aaacgatcga gagggtcagc ggccttcggc cgggctcgag tagagctttg cgagcgtctg
      241 cgttcgcgcg tcgatcgtcg ccgcggcgct ttctaccagc gccggctcgt cggataagtc
      301 gatcccgaga aaacgccgtt ctaacgccgc cggggagtcg tcgaatccat gcttcagcag
      361 cgcgacgtat cgcggtacga aggcctgcgg ggcacgctcg aactccttaa aatacgcgag
      421 cgcgagcaat ccggcgtaca agtagttgac gtcatagagc ggatcggtgt agtagagccg
      481 gttgcgtgcc caatagagtt gcgtcgccgg atccgtaccg gctgccggat cgtagcgcgc
      541 aaagatcttg aggtttaagg cgtcgaaatc ggcggccgtc ttgacggtgc cctcggcaat
      601 gccgcgatag atggcgcttt cgaggtcggt ttcttcggcg gatccgtaaa cttgaaaggt
      661 cgcatcgtcg aggaagtagt tgaggtaata cgcgcgttgc gcgtcggtcg cggcgcgttg
      721 gtaaacgtag ttgaggaaca gaagctcgtt gaagatcgcg aacgactcga acatgaaaga
      781 cggtccgcgg ttgtaggcgg caatcggctg attccgggcc atgaactcgc gatggacggc
      841 gtgacccgac tcgtgggcga cggcgcgaag gctgtgagtt tctccgtcga agttgccgaa
      901 atagacgccc gactccacgc ccgcaaaacc gaccgaaaag ccggtgtcgt cgcattccgg
      961 tgccgtgcaa atttcgagtc gccgattggc gggatcgaag agctcggcat aggcgttcgc
     1021 ataatcggtt cccatcggtc gctcggcggc gagaatcagc gccaaggcat ccgggatggc
     1081 gaagcggccc ggggtaaagg ccggcttcgg cgcttttgcc gcgacgccga ggtagcggcc
     1141 gtaagcgccg gaagcgcgga ccgcacgtaa ggtccgttcg accgatgcgg gatcgatcga
     1201 tcggtcgaag tacgacgcct ccgtcgccgt ttcgaaaccg cgcagccgtg cgacgccgtt
     1261 tctggcgctg ccgatcgaga cgagcagcgc ggcgtacgtg tcctgatggc tctcgatggc
     1321 gccgccggat tttcgtaagg tc
//
LOCUS       DASUCJ010000831         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_131198, whole genome shotgun sequence.
ACCESSION   DASUCJ010000831 DASUCJ010000000
VERSION     DASUCJ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131198"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..678)
                     /locus_tag="VFU90_09955"
     CDS             complement(142..678)
                     /locus_tag="VFU90_09955"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU5480145.1"
                     /translation="MPDNGLVPNAGMSRRIARPPGAATHVVLVQPEIHWNTGNAGRTC
                     LATGSTLHLIEPLGFSLDEREVKRAGLDYWEHVDLHVWPSWDAFERELPDLGDAYFFS
                     TKAERLYWDAPLGAAKSVVLVFGRETAGLPPGLHERYRDRFLRMPMFSPLVRSLNLST
                     SVALALYEVLRQREAMTR"
     gene            complement(653..1285)
                     /locus_tag="VFU90_09960"
     CDS             complement(653..1285)
                     /locus_tag="VFU90_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480146.1"
                     /translation="MTTSEPDPLRAAVDAATAFFGDIDAQRWAAAAHAVDPETALRCR
                     DTALTVLLGWAAMQSVRPTPPDAPAAFEIRMTSGSAQLQRFGSTPLNGVRGVSTLAEL
                     AALTPDEFVAASFEHQHGSQAGDTGSETPAREILAAIADGAFAAQILYRTHRSSEMRS
                     FARPVEQLSMRLRDGRWFVDIEHHEASLSIPRSHVRTPQRDGDAGQRARA"
     gene            complement(1323..>1774)
                     /locus_tag="VFU90_09965"
     CDS             complement(1323..>1774)
                     /locus_tag="VFU90_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480147.1"
                     /translation="LLLLVYAGINALNSRTGEGGDYHSLATLRGVVTGVYTVRNRVEG
                     RRVGVWLDTMPQTEQVQMYGPGVSEADQRVHAIPVGTRVIVHYDGYTQKVWALERADG
                     GPGFSEADVRPWLADAHASERRMMHWLLGAGLVAIVAGIGFMLRDSS"
BASE COUNT          314 a          610 c          566 g          284 t
ORIGIN      
        1 actgcacccg cgcgccgtcc atctttgcgt taggcgctgc gcttgcctgc gcctgaatgc
       61 cggcgcgggc cgtgaacgcc gcgccgagca cggcgagcat gaaggcgcgc cggagtgagc
      121 tgctgcctaa cgttccaaga atcatcgcgt catcgcctcc cgttgtcgca acacctcgta
      181 cagcgccagc gcgacactcg tcgacaaatt gagcgaacgc accagcggcg agaacatggg
      241 cattcgcaga aaccgatcac gatatcgctc gtgcagcccc ggcggcagtc ccgccgtttc
      301 ccggccgaac acgagcacca cgcttttcgc cgcgccgagc ggcgcgtccc aatagaggcg
      361 ctcggccttc gtcgaaaaaa agtacgcatc gccgaggtcc ggcaactcgc gctcgaacgc
      421 atcccagctc ggccagacgt gcagatcgac gtgctcccaa tagtcgagtc cggcacgctt
      481 cacctcgcgc tcgtcgagcg aaaagccgag gggttcgatg agatgcagcg tactgcccgt
      541 tgccagacac gtgcggccgg cattgccggt attccagtga atttcgggct gcaccaggac
      601 gacatgcgtg gccgcacccg gcggccgggc gatgcgccgg ctcatacctg cgttaggcac
      661 gagcccgttg tccggcatcg ccgtcccgct gcggcgtccg cacgtgactt ctcggaatgc
      721 tcagcgaggc ctcgtggtgt tcgatgtcga cgaaccaccg gccgtcgcga agccgcatac
      781 tcagttgctc caccggacgt gcgaacgagc gcatctcgct cgagcggtgt gtccgataca
      841 ggatctgagc cgcgaatgcg ccgtccgcga tcgccgccaa tatctcgcgc gccggcgtct
      901 ccgagccggt atcgcccgct tggctgccgt gctgatgttc gaacgacgcg gcgacgaatt
      961 catccggcgt gagggcagcc aactcggcga gcgtcgagac gccgcggacg ccattcagcg
     1021 gcgtcgatcc aaaacgctgc agttgagccg agcccgacgt catgcggatc tcgaacgcgg
     1081 cgggcgcatc gggcggtgtc ggtcgaacgg attgcatcgc ggcccaaccg aggagcaccg
     1141 tcagcgccgt gtcccggcag cggagcgccg tctccggatc gacggcgtgc gccgcggcag
     1201 cccagcgttg ggcgtcgatg tctccgaaga aggcggttgc ggcatcgacc gccgcgcgca
     1261 gcggatcggg ttcgctcgtc gtcaccggcg gcaggcctac ctgttcgagt gcctggcgac
     1321 gatcacgagg aatcgcgaag catgaaaccg ataccggcca cgatcgcgac gagcccggcg
     1381 ccgagcagcc agtgcatcat gcggcgctcc gacgcatggg cgtcggcgag ccatggccga
     1441 acatctgcct cactgaaccc aggtccaccg tccgctcgct cgagcgccca cactttctgc
     1501 gtgtatccat cgtaatggac aatcacacgc gtgccgaccg gaatcgcatg gacgcgctga
     1561 tccgcttcgg acactcccgg cccgtacatt tgcacttgct cggtctgcgg catggtgtcg
     1621 agccaaacgc cgacgcgtct gccctcgaca cgattcctga ccgtgtaaac tccggtcaca
     1681 acgccgcgca gcgtggcgag cgaatgatag tcgccgcctt cgccggtgcg agaattgagc
     1741 gcgttgatgc cggcgtagac gagcaggagg aggc
//
LOCUS       DASUCJ010000832         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_452124, whole genome shotgun sequence.
ACCESSION   DASUCJ010000832 DASUCJ010000000
VERSION     DASUCJ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452124"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..>1140
                     /locus_tag="VFU90_09970"
     CDS             9..>1140
                     /locus_tag="VFU90_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480148.1"
                     /translation="MTPADLRGWRSIRSPLLSNDGKWFAYILAPNEGDASVIVRQTAD
                     GATEQRFPIGELPAALANPFGPPGGPPALAISGDSRFLAFTIYPTEREARRLRQQRQP
                     VQTKVAIVNLATGQKTEFEKIRRFAFSGEKPRALALYGYAAEAPTATAASERGGPGTA
                     STSRAEGADLLLYSLTNGSIINIGNVGEFAFDESGNYLAYTIDARDQIGNGVQLRDLR
                     SDVVRPLDGEPALYRHLVWADSGPAFAVLRGRPDSAARDTLFSVVAFTAVGSAAQKKV
                     VFDPTGRSDFPSGMVVSADRAPRIADDFATVFFGIHEAKPKQAGVMASRGSSRASIVQ
                     AGAPGEGGARNQPHVDPDDDTPTLVLWHWKDPRLQSQQLVQEQ"
BASE COUNT          196 a          390 c          370 g          184 t
ORIGIN      
        1 ggaagcagat gacccccgcc gacctcagag gctggcgctc gattcgatcg ccgctgctct
       61 cgaacgatgg caagtggttc gcgtacatcc tcgcgcccaa cgagggagac gcttccgtca
      121 tcgttaggca gacggccgac ggtgcgaccg agcagcgctt ccccatcgga gagcttcccg
      181 ccgccctggc caatcccttc gggccgccgg gtggaccgcc cgcactcgcc atctcagggg
      241 attcccgctt cctggcattc acgatctacc cgactgaacg cgaggcgcgc cgactccggc
      301 agcagcgaca acccgtgcaa accaaggtcg cgatcgtgaa tctcgcgaca gggcagaaga
      361 cggagttcga gaagatccgt cgcttcgcgt tcagcggcga gaagccgcgg gccctcgcgc
      421 tttacggcta cgcggccgaa gcgccgacag ccacagcggc ctcggagcgc ggcggtcccg
      481 gaaccgcctc cactagccgt gcggaaggcg ccgatctgct cctctacagc ctgacgaatg
      541 gcagcatcat caacatcggg aacgtcggcg aattcgcctt cgacgagtcg ggcaactatc
      601 tcgcatacac catcgacgcg cgcgatcaga tcgggaatgg tgtgcagctg cgtgacttgc
      661 ggagtgacgt cgtgcgcccg ctcgacggcg agccggcgtt gtatcgtcat ctcgtctggg
      721 cggacagcgg ccccgccttc gccgttctcc gcggtcggcc ggattcggct gccagggata
      781 cgctcttcag cgtcgtcgcg ttcactgctg tcggttccgc ggcgcagaag aaggtcgtct
      841 tcgaccccac ggggcggagt gatttcccgt cgggcatggt cgtgagcgcc gatagggcgc
      901 cgcgcatcgc ggacgatttc gccaccgtgt ttttcgggat tcacgaagcg aagccgaagc
      961 aagccggcgt gatggccagt cgcggctcga gccgtgcctc gatcgtgcag gccggcgcgc
     1021 ccggcgaagg cggtgcgcgc aatcagccgc atgtcgaccc cgacgacgac acgccgacac
     1081 tcgtgctctg gcactggaag gacccgcgcc tgcagtcgca gcaattggtc caggagcaac
//
LOCUS       DASUCJ010000833         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_467434, whole genome shotgun sequence.
ACCESSION   DASUCJ010000833 DASUCJ010000000
VERSION     DASUCJ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467434"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFU90_09975"
     CDS             complement(<1..230)
                     /locus_tag="VFU90_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5480149.1"
                     /translation="MTFRPVVLAVLDGWGCSDAAHGNAIAAAKLPHWRAFFERYPWTT
                     LAASGEAVGLPKGIMGNSEVGHMNLGSGRVVP"
     gene            complement(227..970)
                     /gene="tpiA"
                     /locus_tag="VFU90_09980"
     CDS             complement(227..970)
                     /gene="tpiA"
                     /locus_tag="VFU90_09980"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5480150.1"
                     /translation="MTRTIFAANWKMHKTAAETAAYFDRFLPLAASLPPEFEIVVAPP
                     FTAIAQASQRLAGSNVRLGAQTMHWELEGPFTGEVSAPMLLEFGVSYVILGHSERRAY
                     CNETDRTVNLKVLTALAQGLTPIVAVGETGDERAAGEIDDRVVTQTRDALKGVAQAAL
                     ARVVIAYEPIWAIGTGKNCDAGEADRVMATIRSCLPGLETTPILYGGSMKPENVADYL
                     AKPNVNGGLVGGASLDPDGFATLIANAAR"
     gene            complement(967..2145)
                     /locus_tag="VFU90_09985"
     CDS             complement(967..2145)
                     /locus_tag="VFU90_09985"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5480151.1"
                     /translation="MNVRSVADLDVRGKRVLVREDLNVPMRGPEIADYTRVDAALPTL
                     ALLRERGARTIVMSHLGRPDGTPQPGLSLRPLAAALSERLGAPVGFAPDCVGAAAREA
                     VAALHDGDVVLLENVRFHPEEERNDPAFAARLAELGDLYVNDAFGTAHRAHASTEGVA
                     HLLPSAAGLLMEAELKALARLTENPAKPFVCVIGGAKVKDKIGVFAHLMELVDAFCIG
                     GGMANTFLAARGVAVGRSLRDDDLEPARRVLELAKLHKVDVRLPTDVVIASSFDSGDA
                     HVAAVGDVGEGMILDIGPATAAAYADTVSLAKTIVFNGPMGVYEKPPYRRGTEVVGAA
                     IAAATAKGAFSVVGGGDAAAAAHMLGFASGVSHVSTGGGATLEFLEGKTLPGVAALQR
                     "
     gene            complement(2162..>3027)
                     /locus_tag="VFU90_09990"
     CDS             complement(2162..>3027)
                     /locus_tag="VFU90_09990"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5480152.1"
                     /translation="GLRLIRGAAGDDALLIDERRIKVMAERDPAKLPWKDLGVDVVIE
                     STGFFTDAAKARAHIDGGGAKKVLISAPAKGEDITIVLGVNDSAYDPLKHNVISNASC
                     TTNCLATAVKPIVDRLGWVKGFMTTIHSYTNDQNILDAPHKDLRRARNAATNIIPTST
                     GAAKALYLTIPEVKGTFDGFSLRVPTPTVSMIYLVAQTKKPTDRDALNAILREAAQGE
                     LAKYVAYTEEELVSSDYKKDPHSSIVDAKLTNALGDLVQIGAWYDNEWGYSCRLADLT
                     AMVLEKIPATA"
BASE COUNT          471 a          996 c         1028 g          532 t
ORIGIN      
        1 tgcggaacga cgcggccgct gccgaggttc atgtgtccga cctcgctgtt gcccatgatg
       61 cccttcggca acccgacggc ctcccccgaa gccgccagcg tcgtccaggg gtagcgctcg
      121 aagaaggcgc gccagtgcgg cagtttcgcg gcggcgatcg cgttgccgtg tgcggcgtcg
      181 ctgcagcccc agccgtcgag tacggcgagc acgaccggcc gaaacgtcat cgcgcggcgt
      241 tggcgatcag ggtcgcgaag ccgtcgggat ccagcgacgc gccgccgacc aagccgccgt
      301 tgacgttggg cttggcgaga taatcggcga cgttctcggg cttcatgctt ccgccgtaga
      361 gaatcggcgt cgtctccaaa ccgggtagac agctacggat cgtcgccatc acgcgatcgg
      421 cttcgccggc atcgcagttc ttgccggttc cgatcgccca gatcggctcg taggcgatca
      481 cgactcgcgc gagcgcagcc tgcgctacgc cttttagcgc gtcgcgcgtc tgggtgacga
      541 cgcgatcgtc gatttcaccg gcggcgcgct cgtcaccggt ctcgccgacc gccacgatcg
      601 gcgtcaaccc ttgcgcgagt gccgtcagta ccttcaagtt gacggtgcga tcggtttcgt
      661 tgcagtaggc gcggcgctcg gagtgtccca agatgacgta gctcacgccg aattcgagca
      721 gcatcggggc gctcacctca cccgtaaacg gaccctccag ctcccaatgc atcgtttgcg
      781 cgccgaggcg gacgttcgat ccggcaaggc gctgcgaggc ttgcgcgatc gcggtgaacg
      841 gcggggcgac cacgatctcg aactccgggg gcaacgaggc cgccagcggg aggaagcgat
      901 cgaagtaggc cgcggtctcc gcggccgttt tgtgcatctt ccagttggcg gcaaagatcg
      961 ttcgggtcat cgctgcagcg ccgcaacccc gggcagcgtc tttccttcca gaaactcgag
     1021 cgtcgcgccg ccgccggtcg acacgtgcga gacgccgctt gcgaagccga gcatgtgcgc
     1081 ggcggccgct gcgtcgccgc cgccgaccac gctgaacgca cccttggcgg tggcagcggc
     1141 gatcgccgcg ccgacgactt cggtgccgcg ccggtagggc ggcttttcgt acacgcccat
     1201 cggtccgttg aacacgatcg ttttggcgag cgagacggta tcggcatacg ccgccgcggt
     1261 cgccggcccg atgtcgagga tcattccctc gcccacgtcg ccgaccgcgg ctacgtgtgc
     1321 gtcgccggag tcgaacgaac tcgctatcac gacgtcggtg gggagacgga cgtcgacctt
     1381 gtgaagcttt gccagctcga gaacgcggcg cgcgggctcg agatcgtcgt cgcgcagcga
     1441 ccttccgacg gcgacgccgc gtgccgccaa gaacgtgttc gccattccgc cgccgatgca
     1501 gaacgcgtcg acgagctcca tgagatgcgc gaaaacgccg atcttgtcct taaccttcgc
     1561 gccgccgatg acgcacacga acggcttggc cggattttcc gtgagccgcg cgagcgcctt
     1621 caactcggct tccatcagga ggccggccgc gctcggcagc aagtgcgcca ccccttcggt
     1681 cgacgcgtgc gcgcgatgcg ccgtcccgaa ggcgtcgttg acgtacaggt cgccgagctc
     1741 cgcgagccgt gcggcgaacg ccggatcgtt gcgttcttct tcggggtgaa agcgcacgtt
     1801 ctcgagcagc acgacgtcgc cgtcgtgcaa cgccgcgacg gcctcgcggg ccgcggcgcc
     1861 cacgcagtcg ggcgcaaagc cgaccggcgc gccgagacgc tcggagagcg cagcggcgag
     1921 cggacgcagc gacaatccgg gttgcggcgt cccgtcggga cggccgaggt gcgacatcac
     1981 gatcgttcgc gcgccccgct cccgcagcag cgcgagggtc ggcagcgcgg cgtcgacgcg
     2041 cgtgtagtcg gcgatttccg gaccgcgcat cggaacgttc agatcttcgc gcacgagcac
     2101 gcgctttccg cgcacgtcga gatcggcgac cgagcgaacg ttcatcgagc gaagccgcgc
     2161 tttaggcggt cgcggggatc ttttcgagta ccatcgcggt caggtccgcg agccggcacg
     2221 aataccccca ctcgttgtcg taccaggcac cgatctgcac caagtcgccg agcgcgttgg
     2281 tgagcttggc gtccacgatc gagctgtgcg gatccttctt gtagtcgctc gacaccagct
     2341 cttcttcggt atacgccacg tacttcgcga gctcgccttg cgcggcctca cgcaggatcg
     2401 cgttgagcgc atcgcggtcg gtcggtttct tggtttgcgc caccagatag atcatcgaaa
     2461 cggtcggggt gggaacgcgc agcgagaagc cgtcgaacgt gccctttact tccggaatcg
     2521 tgagatagag cgctttggcg gcgccggtgg aggtcggaat gatgttggtg gccgcgttgc
     2581 gggcgcgccg caagtctttg tggggagcgt cgaggatgtt ttgatcgttg gtgtaggagt
     2641 gaatcgtcgt catgaatccc ttcacccaac cgagccggtc gacgatcggc ttgaccgcgg
     2701 tcgccaggca attcgtggtg cacgacgcgt tggagatgac gttgtgcttg agcgggtcgt
     2761 aggcggaatc gttcacgccc aggacgatgg tgatgtcctc gcctttggcc ggtgccgaaa
     2821 tcaagacttt cttggcgccg ccgccgtcga tgtgggcgcg cgccttggcg gcgtcggtga
     2881 agaagccggt cgattcgatg acgacgtcga cgccgagatc cttccagggg agcttggcgg
     2941 gatcgcgctc ggccattacc ttgatgcgcc gttcgtcgat cagcagcgcg tcgtcgccgg
     3001 cggcgccgcg aatgaggcgc agcccct
//
LOCUS       DASUCJ010000834         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1384418, whole genome shotgun sequence.
ACCESSION   DASUCJ010000834 DASUCJ010000000
VERSION     DASUCJ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384418"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFU90_09995"
     CDS             complement(<1..1066)
                     /locus_tag="VFU90_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5480153.1"
                     /translation="MHVTLLGGGFGRKSKPDLGIEAAVLSKAVDGKPVKVVWTRDDDL
                     HHDYLHTVSVEHLEAALDAKGMPVAWLHRSVAPTIMSTFVPDAKQEADWELAMGVINV
                     PFAVPNIRIENPDAVAHTRIGWFRSVSNIPHAFAVQSFVAECAAAAGRDPKDYLLDVI
                     GPARVVSPKMLGDTWNHGESPERYPVDTGRLRRVAETVARESGWGKTLPKGHGLGIAA
                     HYSFVSYIASVVEVAVDEKGNLVIPRVDIAIDCGATVNPDRVRAQMQGAVVMGIAAAT
                     LGEISFKNGRAQQDNFHQYEVTRMPAAPRDIRVYIIPGDYAKPLGGVGEPGVPPIPPA
                     LCNAIFAATGKRIRKLPIGDQ"
BASE COUNT          171 a          373 c          346 g          191 t
ORIGIN      
        1 gctgatcgcc gatcggcagc ttccggattc gcttccccgt cgctgcgaaa atcgcgttgc
       61 acagcgccgg cggaatcggc ggcacgccgg gctcgccgac accgcccagt ggcttcgcgt
      121 agtcgcccgg aatgatgtag acgcgaatgt cccgcggcgc cgccggcatg cgcgtcactt
      181 cgtactggtg gaagttgtcc tgctgcgccc gtccgttctt gaagctgatc tcgcctagcg
      241 tggccgccgc gatccccatc acgaccgcgc cctgcatttg cgcgcgcact cggtccggat
      301 tgaccgtggc gccgcagtcg atggcgatgt cgacgcgcgg aatgacgaga ttgccttttt
      361 cgtcgaccgc cacttcgacg acgctcgcga tgtaactgac gaagctgtag tgcgcggcga
      421 ttccgagtcc gtggcctttc ggcaacgtct ttccccagcc cgactcgcgc gccaccgttt
      481 cggcgacgcg ccgcaagcgt cccgtatcga cgggatagcg ctcgggcgat tcgccatgat
      541 tccaggtatc gcccagcatc ttcggcgaca cgacgcgcgc cgggccgatc acgtccagca
      601 ggtaatcctt cggatcgcga cccgccgcgg cggcgcactc cgcgacgaac gactgcaccg
      661 cgaacgcgtg cggaatgttc gacaccgagc ggaaccagcc gatgcgcgtg tgggcgaccg
      721 cgtccggatt ctcgatgcgg atgttgggaa cggcgaaggg gacgttgatg acgcccatcg
      781 ccagctccca atcggcttcc tgctttgcat cgggcacgaa cgtcgacatg atcgtcggcg
      841 cgacggaacg gtgcagccag gcgacgggca tgcccttcgc gtcgagcgcg gcctcgagat
      901 gctcgaccga cacggtgtgg agatagtcgt ggtgcagatc gtcgtcgcgc gtccagacca
      961 ccttgaccgg cttcccgtcg accgctttcg acaagaccgc cgcctcgatg cctaagtcgg
     1021 gcttcgactt gcggccgaag ccgccgccca gcagcgtgac gtgcacggtg acgtcgctca
     1081 c
//
LOCUS       DASUCJ010000835         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1201004, whole genome shotgun sequence.
ACCESSION   DASUCJ010000835 DASUCJ010000000
VERSION     DASUCJ010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201004"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="rnc"
                     /locus_tag="VFU90_10000"
     CDS             <1..563
                     /gene="rnc"
                     /locus_tag="VFU90_10000"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5480154.1"
                     /translation="NERMEFLGDSVLGFITASWLFERYGDEAEGLLTLRRAAIVNDVR
                     LAHTARRLGFADVLHLGSGMRTGGGADNTTVLAGAFEAFVAALYLAYGAEKARKFVVD
                     EHVERFDHSVDELIDAKTRLQHYAQEYLSATPVYRETSRGTPQQPAFSSRVSVKGKTL
                     GTGRGPSKKAAQQAAAEAALISLQVR"
     gene            566..>1301
                     /locus_tag="VFU90_10005"
     CDS             566..>1301
                     /locus_tag="VFU90_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5480155.1"
                     /translation="MKLKKIKSFGFKTFADPTTLEFGGGITAIVGPNGSGKSNLVDAF
                     RWVLGETSSRSLRGGKLEDVIFAGNDARKPLGLAEVSVTFDNTGGRLPIEFSEVEITR
                     RAYRAGESEYFINRTQVRLRDILDLLMGTGLGPGSYAIVSQGQVDAILTSKPADRRAL
                     FEETAGINKFLARKNESLRRLEHTEQNAIRISDLISELEHRVPELETQVRRAKRYRKV
                     SARVRDLEILGYLRAGASRRAERDGLR"
BASE COUNT          249 a          405 c          440 g          207 t
ORIGIN      
        1 ccaacgagcg gatggagttc ttgggcgatt cggtgctcgg attcatcacc gcgagctggc
       61 tgttcgaacg ttacggtgac gaagcggaag gcctgttgac gctgcgccgg gcggcgatcg
      121 tcaacgacgt gcggctcgcg catacggcgc gccggctggg attcgcggac gtcctgcacc
      181 tgggcagcgg gatgcgcacc ggcggcggcg ccgacaacac gaccgtgctg gccggcgcgt
      241 tcgaagcgtt cgtggcggcg ctctatctgg cgtatggagc cgaaaaagcg cgcaaattcg
      301 tcgtcgacga gcacgtcgag cgcttcgacc actcggtcga cgagctgatc gacgcgaaga
      361 cgcgcctgca gcattacgcc caagagtatc tgtccgcgac gccggtgtat cgcgagacca
      421 gccgcggaac gccgcagcaa ccggccttca gctcgcgcgt gagcgtcaaa gggaagacgt
      481 tgggaacggg ccggggtccc tcgaagaaag ccgcacagca ggccgcggcc gaagcggcgc
      541 tgatctcgtt gcaggtgcgt tgagggtgaa actcaagaag atcaagtctt tcggattcaa
      601 gacgtttgcg gatccgacca ccttggagtt cggcggcggc attaccgcga tcgtgggacc
      661 gaacggctcg gggaaatcca atctggtcga cgccttccgc tgggtgctcg gcgaaacgtc
      721 gagccgcagc ctgcgcggcg gcaagctcga agacgtcatc ttcgccggta acgacgcccg
      781 caaaccgctc ggcctcgccg aagtttcggt gacgttcgac aataccggcg ggcggctgcc
      841 gatcgagttc tccgaagtcg aaatcacgcg tcgcgcgtac cgcgccggcg aaagcgaata
      901 cttcatcaat cgcacgcagg tgcggctgcg cgacatcctc gatctgctga tgggcaccgg
      961 cctcgggccg ggctcgtatg cgatcgtgtc gcagggccaa gtcgacgcga tcctcaccag
     1021 caaaccggcc gaccggcgcg cgttgttcga agaaacggcc ggcatcaata aattcctggc
     1081 ccgcaagaac gagtcgttgc ggcgcctcga gcacaccgaa cagaatgcga tccgcatcag
     1141 cgatctcatt tcggaactgg aacaccgcgt accggagctc gagacgcagg tgcggcgggc
     1201 taaacgctat cgcaaggtga gcgcgcgagt gcgcgacctc gagattctcg gatatctgcg
     1261 cgcgggagcg tcgcggcgcg ccgagcgtga cggcctgcgc t
//
LOCUS       DASUCJ010000836         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1247018, whole genome shotgun sequence.
ACCESSION   DASUCJ010000836 DASUCJ010000000
VERSION     DASUCJ010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247018"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>1300
                     /locus_tag="VFU90_10010"
     CDS             209..>1300
                     /locus_tag="VFU90_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5480156.1"
                     /translation="MSRSLFGTRVRAALVFSAIVCLGAAPAQSLSPQLAEDVNQSYRL
                     LTASAYQRVDPQTLLASASDALAAEARKHGVTLTPPALRAESYDATTAELDDAIASSA
                     AAAHAAPTDFAYAAIAAMAKSIGDRYTQFFTPDEFKQFNAALDPDRISGIGVMIQPDA
                     VTKEIRLSYVVPGTPADRTGLRAGDLLTAIDTTSTKGLTVEEASKLLRGKAGTTVDVA
                     IERDGASDATYTIKREEVQPPTVVFKLLPGSIGYVYVMAFGKATPMEFDTAISRLKDQ
                     GAKALVLDLRNDGGGYVDSALLISRRFIANKTLLTVEERGNRATTIDADNDTSVAIPV
                     TVLVNQYTASASEITAGALQDDGIGMLVGT"
BASE COUNT          229 a          433 c          428 g          210 t
ORIGIN      
        1 ggccggtctg ggcgtcgcga tcatggaccg gctgcgccgc gcggcgagcc cttagggttg
       61 cccggccggc cgagcggcct gcccggtgct tttcgaaacg gccgttttgc gctacgctcg
      121 tgccgggaac gactcgggcg cttctttaac gagaacctcg ccgaaacgga cggggctttt
      181 cgtacgttgc gccataaaag aaggaacgat gtcaagatct ttgtttggca cgcgcgttcg
      241 tgccgcgctc gtattctcag cgatcgtctg tctcggtgcc gcgcccgcgc agagcttgtc
      301 cccgcaactc gcggaggacg tgaatcaaag ctaccgtctg ctgacggcca gcgcgtacca
      361 gcgggtcgac ccgcagacgc tgctcgcctc cgcgagcgac gcactcgcgg ccgaagcgcg
      421 taagcacggc gtgacgctga cgccgccggc gctgcgcgcc gaaagctacg acgcgacgac
      481 ggccgagctg gacgacgcga ttgcgtcgtc ggccgccgcc gcccacgccg ctccgaccga
      541 tttcgcctac gcggcgatcg cggctatggc caaatcgatc ggcgaccgct acacgcagtt
      601 cttcacgccc gacgagttca aacagttcaa cgcggcgctc gatcccgatc ggatcagcgg
      661 aatcggcgtg atgattcagc ccgacgcggt gacgaaagag atccggctct cgtacgtcgt
      721 gccgggcacg ccggcggatc gcaccggcct gcgcgccggc gatttgctga ccgcgatcga
      781 cacgacctcc accaaagggc tgaccgtcga ggaggccagc aaactcctgc gcggcaaggc
      841 cggcacgacg gtcgacgttg cgatcgagcg cgacggcgcc tccgacgcaa cctacacgat
      901 caagcgcgag gaagtgcagc cgccgacggt cgtcttcaag ctgctgccgg gatcgatcgg
      961 ctacgtctac gtcatggcgt tcgggaaggc gacgccgatg gagttcgata ccgcgatatc
     1021 gcggctaaag gatcagggcg ccaaggcgct ggtcctggat cttcgcaacg acggcggcgg
     1081 ctacgtcgac tcggcgctcc tgatcagccg gcgcttcatt gccaacaaga cgctgctcac
     1141 cgtcgaagag cgcggcaatc gcgccaccac gatcgacgcc gacaacgaca cgtcggtggc
     1201 gattccggtc acggttttgg tgaatcagta caccgcttcg gcttcggaga ttacggccgg
     1261 tgccctgcag gacgacggga tcgggatgct ggtcgggacg
//
LOCUS       DASUCJ010000837         1451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_253265, whole genome shotgun sequence.
ACCESSION   DASUCJ010000837 DASUCJ010000000
VERSION     DASUCJ010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253265"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1451)
                     /locus_tag="VFU90_10015"
     CDS             complement(<1..>1451)
                     /locus_tag="VFU90_10015"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5480157.1"
                     /translation="CVGGLLFGLVIYRQLQALPVHASMREVSELIYETCKTYLVQQGK
                     FLLLLEIFIGVVILLYFGVLQHYGAAKVVIILLFSLLGIAGSYGVAWFGIRVNTFANS
                     RTAFASLRGKPYPCFSIPLSAGMSIGMLLISVELVMMLCILLFSPGDYAGPCFIGFAI
                     GESLGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGDNAGDSVGP
                     SADGFETYGVTGVALITFIVLAVKEPAVQVQLLVWIFVMRVVMIVASGASYLINGWVA
                     KARYAAADKMNFEAPLTSLVWLTSIVSIVLTYVVSYLLLPRLGDGTMWWKISTVMTCG
                     TLAGALIPELVKVFTSVDSIHTREIVSSSREGGASLNILSGLVAGNFSAYWLGIVIMG
                     LMAIGAWVASFGMDALMVAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSIFEL
                     SLIEQEPNIAAELTRDFGFAPQFARSKDLLEEN"
BASE COUNT          308 a          494 c          428 g          221 t
ORIGIN      
        1 gttctcctcg agcagatcct tcgatcgcgc gaactgcggc gcgaatccaa agtcgcgcgt
       61 gagctcggcc gcgatgttcg gctcttgctc gatcaacgac agctcgaaga tcgactgcgc
      121 gttgtcggtc accggaccat acgagtcgac cgcgatcgtc accggaccca tgccgaggaa
      181 cccgaacgcg accaggccga acgcgaacac ggccggcgcc accatgagcg cgtccatgcc
      241 gaagctcgcc acccaggcgc cgatcgccat gaggcccatg atcacgatgc ccaaccagta
      301 ggcgctgaag ttccccgcca ccaagccgga cagaatgttg agcgacgccc cgccttccct
      361 ggaggagctc acgatctcgc gcgtgtggat cgagtcgacc gacgtgaaca ccttcaccag
      421 ctcggggatc aacgcgccgg ctagtgtgcc gcacgtcatc accgtcgaaa tcttccacca
      481 catcgtgccg tcgcccaacc gcggcagcag cagatacgag acgacgtagg tgagcacgat
      541 cgatacgatc gacgtcaacc acaccagcga cgtcagcggc gcctcgaagt tcatcttgtc
      601 ggccgccgcg taccgcgcct tcgccaccca gccgttgatg agatacgacg cgccgctggc
      661 gacgatcatc acgacgcgca tcacgaagat ccacaccagg agctgcacct gcaccgccgg
      721 ctcttttacc gcgagcacga tgaacgtgat cagcgcgacg cccgtcacgc cgtacgtctc
      781 gaacccgtcg gcgctcggcc caacggagtc gccggcgttg tcgcccgtgc agtccgcgat
      841 cacaccgggg ttgcgcgcgt cgtcttcctt gatgttgaac acgatcttca tcaagtcgga
      901 gccgatgtcc gcgatcttgg tgaagatgcc gccggcgata cgcagcgccg ctgcgcctaa
      961 cgattcgccg atcgcgaacc cgataaagca cgggccggcg tagtcgcccg ggctgaagag
     1021 caggatgcag agcatcatca ccagctcgac gctgatgagc agcatgccga tgctcatgcc
     1081 ggcgctcaac ggaatcgaga agcagggata cggcttcccg cgcagactcg cgaacgccgt
     1141 ccgggaattc gcgaacgtgt tcacgcgaat gccgaaccac gccacgccgt agctgccggc
     1201 gatgcccaac agactgaaca acagaatgat caccactttg gcggcgccgt agtgctgcag
     1261 cacgccgaag tacagcagga tgacgacgcc gatgaagatt tcgagcagga gcaggaattt
     1321 gccctgctgc accagatagg tcttgcaggt ctcgtagatc agctccgaca cctcgcgcat
     1381 ggaggcatgc accgggagcg cctgcagctg gcggtagatc acgaggccga acaggaggcc
     1441 gccgacgcac a
//
LOCUS       DASUCJ010000838         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_192049, whole genome shotgun sequence.
ACCESSION   DASUCJ010000838 DASUCJ010000000
VERSION     DASUCJ010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192049"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="VFU90_10020"
     CDS             complement(<1..>1025)
                     /locus_tag="VFU90_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480158.1"
                     /translation="FGGSLVFAVAATALMYRINPALTLVVLVPLVLAAGVVNILGGTI
                     RTYRRASRAATSRVTGFIGELFGAVQAVKVASAEESVVGAFRRLNADRRRFALKDNLI
                     TESVDSFNQNTGQLAIGVMLLVIAASLRAGTFTVGDFALFASYLGWINDFPRYGSRVL
                     TRYRHAGVSFERMLRILRGAPPETLVDHGPVYLSGAFPPVPHVAKSDGHRLDLLEAVG
                     LTYRHARSGRGIAGIDLRLPRGSFTVITGRIGSGKSTLLRVLLGLLARDAGEIRWNGE
                     TIADPARFLVPPRAAYTPQAPRLFSETLGDNILQGLPMDRVDPAEAIRLAVLERDVAA
                     MPRGLET"
BASE COUNT          187 a          341 c          356 g          141 t
ORIGIN      
        1 cgtctccaga ccgcgcggca tcgcggcgac gtcccgctcg agcaccgcca ggcgaatcgc
       61 ctcggccggg tcgacgcgat ccatcggcag cccttgaagg atgttatccc cgagcgtctc
      121 gctgaagagc cgaggcgctt gcggggtgta ggccgcgcgg ggcgggacga ggaagcgcgc
      181 cgggtcggcg atggtctccc cgttccagcg gatctctccc gcgtcgcgcg ccagcaagcc
      241 gagcaggacg cgcagcaagg tggacttgcc ggagccgatc cgcccggtaa tcacggtgaa
      301 cgagccgcgc ggcaggcgca ggtcgatccc cgcgatgccg cgcccggatc tcgcgtgccg
      361 ataggtcagc ccgacggcct ccagcagatc gagccggtgc ccgtcggact tggcaacgtg
      421 gggaaccggg gggaacgcgc cactcaagta gaccggtccg tggtcgacga gcgtttccgg
      481 cggcgcgccc ctgagaatcc gcagcatgcg ctcgaacgag acgccggcgt gtcggtaacg
      541 ggttaagacg cggctcccgt agcgcgggaa gtcattgatc cagccgaggt agctggcgaa
      601 cagcgcgaaa tcgcccacgg taaacgtacc ggcccgcagc gacgcggcga tgaccaggag
      661 catcacgccg atcgcaagct gaccggtgtt ctggttgaac gaatcgaccg actcggtgat
      721 caggttgtct ttcagcgcga atcggcgacg gtcggcgttg agtcgccgaa acgcgccgac
      781 gaccgattcc tcggccgagg cgactttcac cgcctgcacc gcgccgaata gctcgccgat
      841 gaagccagtc acacgactgg tcgccgctcg actcgcccgt cggtacgtgc gaatcgttcc
      901 gcccaggatg ttgacgacgc cggccgcgag tacgagcgga accagcacga cgagagtcag
      961 cgccggattg atccggtaca tgagagccgt cgcggcgacg gcgaagacga gcgagccgcc
     1021 aaagt
//
LOCUS       DASUCJ010000839         1476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_895402, whole genome shotgun sequence.
ACCESSION   DASUCJ010000839 DASUCJ010000000
VERSION     DASUCJ010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895402"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFU90_10025"
     CDS             <1..1069
                     /locus_tag="VFU90_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5480159.1"
                     /translation="AAELRAQLETTPDDQKQPIEAKLAKINADNTADAQIKNDARFPY
                     PHHTHRAEYETATAFGTMMLNNDLNSLQYANHLCNEYGFDTIAAGSTLSFAMECFENG
                     LITKEDTDGIDLRWGNSEAIIEALHKIGRKDGAFGELFGDGVRKASEKIGPKSVEFAM
                     EVGGEELPMHDAKLQPEYFTTYKLDATPARHTQYEGAGRPGWGFRKPPQDRTVATERG
                     PAHKDAAEYMHIVNATGMCQFIMMCAPNDRIPEWIGIETGWDTSVDEMRTVGERIANM
                     RMAFNVREGDLVTKRRIPARLSGAAPLQAGPHKDVSLDMNTLEQDYLAACDWDADTAK
                     PSKRKLESLGLGDVAEKIAAK"
     gene            1144..>1476
                     /locus_tag="VFU90_10030"
     CDS             1144..>1476
                     /locus_tag="VFU90_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480160.1"
                     /translation="MEHDKDQRSAMKQRIIAAARTDDRIVGLVDYGSGGEGRADDLSD
                     VDVAVFVRDDAYDAFARDWQVWAAQFGSPLLAYRGRYRHPWCVYDTQPVPLRVDFDLH
                     RASHAGRVI"
BASE COUNT          278 a          472 c          530 g          196 t
ORIGIN      
        1 ggccgccgag ctgcgcgcgc agcttgagac gacgcccgac gaccagaagc agccgatcga
       61 ggccaagctc gcgaagatca acgccgacaa cacggcggac gcgcagatca agaacgacgc
      121 gcgcttcccg taccctcatc acacgcaccg tgccgagtac gagacggcga ccgcgttcgg
      181 cacgatgatg ctgaacaacg acctcaactc gctgcagtac gcgaaccacc tctgcaacga
      241 gtacggcttc gacacgatcg ccgcgggctc gacgctgtcg ttcgcgatgg agtgcttcga
      301 gaacggcctg atcacgaagg aagacaccga tggcatcgac ctgcgctggg gcaacagcga
      361 ggcgatcatc gaggcgctgc acaagatcgg ccgcaaggat ggcgcgttcg gggagttgtt
      421 cggcgacggg gtccgcaagg cgtcggagaa gatcggcccg aagtcggtcg agttcgcgat
      481 ggaggtcggc ggcgaggagc tgccgatgca cgacgcgaag ctccagcccg agtacttcac
      541 gacgtacaag ctcgacgcga cgccggcgcg ccacacgcag tacgagggcg ccggccggcc
      601 cggctggggc ttccgcaagc cgccgcagga ccgcacggtc gcgacagagc gtgggcccgc
      661 gcacaaggac gcggccgagt acatgcacat cgtcaacgcg acggggatgt gccagttcat
      721 catgatgtgc gcgccgaacg accggatccc cgagtggatc gggatcgaga ccggctggga
      781 cacgagcgtc gacgagatgc ggacggtggg cgagcgcatc gcgaacatgc gcatggcgtt
      841 caacgtgcgc gagggcgacc tcgtcacgaa gcggcggatc ccggcgcggc tcagcggcgc
      901 ggcgccgctg caggccggcc cgcacaagga cgtctcgctc gacatgaaca cgctggagca
      961 ggactacctt gcggcgtgcg actgggacgc cgacacggcg aagccgagca agcgcaagct
     1021 cgagtcgctg ggcctcggcg acgtggccga gaagatcgcg gcgaagtagc cgcggccagc
     1081 aagttagcgg tgtagaagaa gggcctcggt agaggccctt cttgcgtttg aggcggtgcc
     1141 gtcatggagc acgacaagga ccagcgttcg gccatgaagc agcggatcat cgctgcggca
     1201 cgcaccgatg accggatcgt tgggctcgtc gactacgggt cgggcggcga gggccgcgcg
     1261 gacgatctgt cggacgtgga cgtggccgtg ttcgtgcgcg acgacgcgta tgacgcgttc
     1321 gcgcgcgact ggcaggtgtg ggcggcgcag ttcggttcgc cgctgctcgc gtatcgcggc
     1381 cgctaccggc acccgtggtg cgtctacgat acgcagccgg tgccgctacg cgtcgacttc
     1441 gacctgcacc gcgcgtcgca cgctggccgc gtcatc
//
LOCUS       DASUCJ010000840         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_650940, whole genome shotgun sequence.
ACCESSION   DASUCJ010000840 DASUCJ010000000
VERSION     DASUCJ010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650940"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFU90_10035"
     CDS             complement(<1..1010)
                     /locus_tag="VFU90_10035"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEU5480161.1"
                     /translation="MIILIAWTGLTRPAFMSALAALASAPVLYGIFSRGGHASAGWEC
                     LIVTIPVAAALGEILSWSMRRARKMTHLEAQRRLHDALTGAGNRTLLSVQLDHALARV
                     RRSHKALALLQVNLDDFKSVNETMGTFAGDDVLVETAARLRASIRETDTIARIGADEF
                     VVLCEDIDALHFATTIARRILDALSLPFTVESDSVRLTASIGIAFSSDGTETVDTLLQ
                     NAGLALYQAKHSGRGRYEVFGEAIRDQIAKRRELEAALARAVSRQELRLHYQPIVESE
                     SARVESFEALLRWERPGLGVIPPGDFIPLAEQTGLIVDIGAWVVQEACRQAASWGNER
                     LRS"
BASE COUNT          243 a          351 c          376 g          207 t
ORIGIN      
        1 gatcgcaagc gttcgttacc ccaactcgct gcttgcctac atgcttcttg gaccacccat
       61 gcgccgatgt cgacgatgag cccggtctgt tcggcgagcg gaatgaaatc gccgggcgga
      121 atcacaccga ggcccgggcg ctcccagcga agcagcgcct cgaagctttc gacgcgagcg
      181 gattcgcttt cgacaatcgg ctgatagtgc agccgcaact cttggcggga caccgcacgc
      241 gcgagcgctg cttcgagttc gcgccgcttt gcgatttgat cgcggatagc ttccccgaag
      301 acttcgtagc gcccgcggcc ggagtgtttc gcttggtaga gcgcgagtcc cgcgttctgc
      361 agcaatgtgt ctacggtttc ggttccgtcg gacgagaacg cgatgccgat actcgcggtg
      421 agccgtaccg agtccgactc aaccgtgaac ggcaacgaga gcgcgtccag aatccgccga
      481 gcgatcgtcg tcgcaaagtg cagcgcgtcg atgtcctcgc acagcacgac gaactcgtcg
      541 gcaccaattc tcgcgatggt gtcggtctct cgtatcgagg cacgcagacg cgcagccgtc
      601 tcgacgagaa cgtcgtctcc ggcgaacgtc cccatcgttt cgttcacgct tttgaagtcg
      661 tccagattga cttgaagcaa tgcaagtgct ttgtggctgc gacgaacacg cgcaagtgca
      721 tggtcgagtt gaacggagag gagcgtccgg ttgccggcgc cagtgagcgc gtcgtgcaat
      781 cgccgttgcg cctcgagatg cgtcatcttg cgcgcgcgac gcatcgacca agaaaggatc
      841 tcgccgagtg ccgcggccac gggaatcgtg acgatgaggc attcccaccc ggccgaagcg
      901 tgcccgccgc gggagaaaat tccgtagaga acaggggcgg aagcgagcgc ggcaagcgcg
      961 gacatgaagg ccggacgcgt gaggccggtc caagcgatca ggatgatcac gaatacggga
     1021 tatacggcca gagctgattc agttctcgag tagtggttca cggcgtctgt gacgaacaag
     1081 aacgcgagcg tcaagacgac gatcgagttc gcgacggccg tgaaatgacg cttccaggga
     1141 aggacgagaa cgaccgccgc gatcagcaaa cagctga
//
LOCUS       DASUCJ010000841         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_528601, whole genome shotgun sequence.
ACCESSION   DASUCJ010000841 DASUCJ010000000
VERSION     DASUCJ010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528601"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1127
                     /locus_tag="VFU90_10040"
     CDS             <1..>1127
                     /locus_tag="VFU90_10040"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEU5480162.1"
                     /translation="YCENALEAIGHTPLVKLNKVTDGAECLVLAKVEYVNPGGSVKDR
                     PAIAMLDAAEKQGLLKPGGTIVEPTSGNTGTGLAMAAAIRGYRCILVMPDKMSKEKID
                     LLRAYGADVVITPTNAPADSPESYYGVANRLASEIPGAFQPNQFHNQFNPEAHYHTTG
                     PEIWEQTAGTITHFVAGIGTGGTISGTARFLKERNPKIHVVGADPEGSIYSGDIPRSY
                     AVEGIGMNYLPETVDLRVIDEVIRVSDRDSFLLARRIAREEGLLVGGSSGTAGVAAVK
                     LAKTLPKDAVVVVIFPDSGRGYMSKIFSDDWMIANGFLADAKRRATVGDVLRSKAPLP
                     PLITVQYDDTVKTALDLLRKYEISQVPVMREHEHVGSVNDV"
BASE COUNT          225 a          381 c          336 g          185 t
ORIGIN      
        1 cgtactgcga aaacgcactg gaagccatcg gccatacgcc cctcgtcaag ctcaacaaag
       61 ttaccgacgg cgccgagtgt ctcgtccttg ccaaggtcga atatgtgaat cccggcggca
      121 gcgtgaagga tcggccggcg atcgcgatgc tcgacgcggc cgagaagcaa gggctgctca
      181 aacccggggg gacgatcgtc gagccgacca gcggcaacac cggaaccggg ctcgcgatgg
      241 ccgccgccat ccgcgggtac cgctgcatcc tcgtcatgcc cgacaagatg tccaaggaga
      301 agatcgacct attgcgcgcc tacggcgccg acgtcgtcat cacgccgacc aacgctccgg
      361 ccgattcccc cgagtcgtac tacggggtcg ccaatcggct cgcaagcgag atccccggcg
      421 cctttcagcc caaccagttc cacaatcaat tcaaccccga ggcgcactac cacacgaccg
      481 gccccgaaat ttgggaacaa accgccggca cgatcaccca cttcgtcgcc ggcatcggga
      541 ccggcgggac gatctccggt acggcgcgtt ttttgaagga gcgcaaccca aagattcacg
      601 tcgtcggtgc cgatccggaa ggctcgatct actccggcga cattccccgg tcgtacgccg
      661 tcgaaggcat cggcatgaac tacttgcccg aaaccgtcga cctgcgcgtg atcgacgaag
      721 tcattcgggt gtccgatcgc gactcgttct tgctcgcgcg ccggatcgca cgcgaagagg
      781 gcttgctggt cggcggatcg tcgggtaccg ccggcgtcgc ggccgtgaag ctcgccaaaa
      841 cgcttccgaa agatgcggtc gtggtggtga tctttcccga ctcgggacgc ggctacatgt
      901 cgaagatttt cagcgacgac tggatgatcg ccaacggctt cttggccgac gccaagcggc
      961 gcgccaccgt cggcgacgta ctccgctcga aggcgccgct gccgccgttg atcaccgtac
     1021 agtacgacga taccgttaag acggcgctcg atctcctgcg caagtacgag atctcgcagg
     1081 ttccggtcat gcgcgaacac gagcacgtcg gcagcgtcaa cgacgtc
//
LOCUS       DASUCJ010000842         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_757737, whole genome shotgun sequence.
ACCESSION   DASUCJ010000842 DASUCJ010000000
VERSION     DASUCJ010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757737"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFU90_10045"
     CDS             <1..935
                     /locus_tag="VFU90_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5480163.1"
                     /translation="IEAIEAARERIAGTILRTPLVKLQTGEGEPEIYLKLENLQPINS
                     FKLRGAANAVAMLPPQRRALGVWTISAGNAGQGVAYAARAAGVPCTVVTIETAPETKV
                     SRMRALGATIVKASFDACWEAMERRTFPGVDGTFVHPFDDDDFIAGNATMGLEIVEDL
                     PNVATVVTAMGGGGLATGVASGVRARKPDVRVLVAEPETAAPASLSFQTGVASPFPQW
                     EATFVDGAGGKSVFPRMWERMRPVVDGAIVVSLDETKRAMRVLAEKARVIAEGAGALA
                     VAAALSGKAGDGPIVAVVSGGNIDLETFCRLIGS"
     gene            complement(1024..1527)
                     /locus_tag="VFU90_10050"
     CDS             complement(1024..1527)
                     /locus_tag="VFU90_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU5480164.1"
                     /translation="MPSTISLGSTGNDVKRLQRVLARHLMWNPFGPITGTFDAGLETA
                     VKTFQQSNGLTVDGIVGPATWAALPAYREASPVLEKGAHGPAVAWLQKALSGADVAIS
                     FAPYAGALDGVFGPQTDTAVRALQTWAGTTVDGIVGDDTWFTWMTPGSAQQLTLEGAC
                     GLTNGLL"
     gene            complement(1593..3068)
                     /locus_tag="VFU90_10055"
     CDS             complement(1593..3068)
                     /locus_tag="VFU90_10055"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEU5480165.1"
                     /translation="MRAEAGKTAAAANARSAVPLHQRKRPRDSAFADTYATRAMDEFV
                     PKRKIPETGLRADVAYQLVTDELMLDGNARQNLATFCQTWFDDEIHKLMDLSLDKNMI
                     DKDEYPATAEIEGRCVHLLADLWNSPAAATTIGCSTTGSSEAAMLGGLALKWKWRERR
                     KAAGKPADKPNLITGPVQVCWHKFARYFDVELREVPLERDRLVMTPAEVLARVDENTI
                     GVVPTFGVTFTCQYEPVAEIAAALDQLERDTGLDVPIHVDAASGGYIAPFVRPDVVWD
                     FRLPRVRSINTSGHKFGLAPLGVGWVIWREASDLPEGLIFHVNYLGGDMPTFALNFSR
                     PGGQVVCQYYLFLRLGMDGYRRIHQACYDVAQYIGRHIAGFGPFEILFDGDSLNGIPA
                     VSWTMREDAGYSLFDLSDRLRSRGWQVAAYTMVPNVSDMAVMRVLVRHGFSHDFADGL
                     IDDMRRAIEYFKKHPVSQPMLASEGASHHHGSRAKQPVATG"
BASE COUNT          569 a         1046 c         1105 g          559 t
ORIGIN      
        1 cgatcgaggc gatcgaggcg gcgcgcgagc gaattgccgg aacgattctg cggacgccgc
       61 tggtcaagct ccagaccggc gagggcgagc cggagatcta cctcaagctc gaaaatcttc
      121 agccgatcaa ttcgtttaag ctgcgcggag cggccaacgc ggtcgcgatg cttccgccgc
      181 aacggcgcgc gctcggcgtt tggacgatta gcgccggcaa cgccgggcaa ggcgtcgcgt
      241 acgcggctcg cgccgccggc gtgccgtgca cggtcgtcac gatcgaaacc gcgccggaaa
      301 cgaaggtctc ccgaatgcgc gcgctgggcg cgaccatcgt caaggcgtcg ttcgacgcct
      361 gctgggaagc gatggaacgg cgcaccttcc ccggcgtcga cggcacgttc gtgcatccgt
      421 tcgacgacga cgacttcatt gcgggcaacg ccacgatggg tttggagatc gtggaggatc
      481 ttccgaacgt cgcaaccgtc gtcaccgcaa tgggcggcgg cggcctcgcg acgggggtcg
      541 cttcgggcgt tcgcgcgcgc aaaccggacg ttcgcgtgct ggtcgccgag ccggaaacgg
      601 ccgcgccggc gtcgctctcc tttcaaaccg gcgtcgcgtc gccgtttccg cagtgggagg
      661 cgacgttcgt cgacggtgcc ggcggcaaga gcgtgttccc gcggatgtgg gaacgcatgc
      721 gcccggtggt cgacggcgcg atcgtcgtca gtctcgacga aacgaaacgt gcgatgcgcg
      781 tgctggccga aaaggcgcgc gtcatcgccg aaggcgccgg agcgctggcc gtcgccgcgg
      841 cgttgagcgg aaaggccggc gacgggccga tcgtggccgt cgtgtcgggc ggcaacatcg
      901 atctggaaac gttttgtagg ctgatcggaa gctagcgcga ttatcgcgcg atgatcttga
      961 cgatctggcc gtcgggccag aacggatccg atcttcccag gtccggcccg ggcgtgcggg
     1021 gcgctagagc aagccgttcg tcaggccgca ggccccttcg agcgtgagct gctgcgccga
     1081 tccgggcgtc atccacgtga accaggtgtc gtctccgacg atcccatcga cggtcgtgcc
     1141 ggcccaggtt tgcagcgcac gcacggccgt gtcggtttgc ggcccgaaga cgccgtcgag
     1201 tgcgcccgcg tacggcgcga acgaaatcgc cacgtcggcg ccgctgaggg ctttctgcaa
     1261 ccacgcgacc gcgggaccgt gcgcgccctt ctcgagcacc ggtgaggctt cgcgatacgc
     1321 cggaagcgcc gcccacgtcg ccgggccgac gatgccgtcg acggtaagtc cgttgctctg
     1381 ttgaaacgtt ttgaccgcgg tttccaaacc cgcgtcgaag gttccggtga tcggaccgaa
     1441 cggattccac atcaggtggc gcgcaagcac gcgctgcagt cgtttgacgt cgttcccggt
     1501 cgagcccagg ctaatcgtag acggcatgat tcatccctcc cgtggggccc agtatacgac
     1561 gccgagcgcg tcccgagaat gggccgtcgg aatcagcccg tcgcgacggg ttgtttggcg
     1621 cgcgaaccgt gatgatgcga ggcgccttcg gaggcgagca tcggctggga taccgggtgc
     1681 tttttgaaat actcgatcgc acggcgcatg tcgtcgatca aaccgtcggc gaaatcgtga
     1741 ctaaagccgt ggcgcacgag cacgcgcatc accgccatat cgctgacgtt cggcaccatc
     1801 gtgtacgccg ccacctgcca gccgcgcgag cgcagccggt cggacagatc gaacagcgaa
     1861 tagccggcgt cctcgcgcat cgtccacgaa acggccggga ttccgttcaa actatcgccg
     1921 tcgaacagta tttcgaaggg gccgaatccg gcgatgtgcc gcccgatgta ctgcgcgacg
     1981 tcgtagcagg cttgatggat gcggcgatag ccgtccatcc cgagccgtaa gaacaggtag
     2041 tactggcaaa cgacctgtcc gcccggccgc gagaagttca gcgcaaacgt cggcatgtcg
     2101 ccgccaagat agttcacgtg gaagatcaag ccttcgggca gatcgctcgc ttcgcgccag
     2161 atcacccatc caacgcccag cggcgccagc ccgaacttat gccccgacgt attgatcgag
     2221 cgaacgcgcg ggaggcggaa gtcccacacg acgtccgggc ggacgaacgg cgcgatgtag
     2281 ccgccggagg cagcgtcgac gtgaatcggc acgtcgagcc cggtatcgcg ctcgagctgg
     2341 tcgagcgccg cggcgatctc cgcgaccggc tcgtactggc acgtaaacgt cacgccgaac
     2401 gtggggacga cgccgatcgt gttctcgtcg acgcgcgcga gcacttcggc gggggtcatc
     2461 accaagcgat cgcgttccag cggaacctcg cgcagctcga cgtcgaaata gcgggcgaac
     2521 ttgtgccaac acacttgcac cgggccggtg atgaggttgg gcttgtcggc cggtttgccg
     2581 gcggccttgc ggcgttcgcg ccacttccac ttcagcgcga gcccgccgag catggccgcc
     2641 tcgctcgatc cggtggtgga acagccgatc gtcgtggcgg ccgccggcga gttccacaaa
     2701 tcggcgagca gatgcacgca gcggccttcg atttcagcgg tggccggata ctcgtccttg
     2761 tcgatcatgt tcttgtcgag actgagatcc attaatttat gaatctcgtc gtcgaaccac
     2821 gtctggcaga acgtagcgag attttggcgt gcgttgccgt cgagcatcag ctcgtcggta
     2881 acgagctggt aggcgacgtc ggcacgcagg ccggtctcgg ggattttgcg cttgggaaca
     2941 aattcgtcca tcgcccgcgt cgcgtaggtg tcggcgaaag cggaatcgcg cggacgttta
     3001 cgttgatgta acggcacggc agatctggcg ttagccgcgg cggcggtttt tcccgcctcg
     3061 gcacgcacta gagcacgagc gtcagccccg cttccacatg gcgctcgccg acgtagacga
     3121 ggttcaggcg atcgtgaaga gtttccagcc ggcttcctgt gcttgctgaa cggcggccac
     3181 gccggccggg accatctgaa aaccgggcag catgtttgcc gaaaaatcgg cgagcacggc
     3241 cttcgggtcg ccgccgctct gcatggtggt gaccaacgc
//
LOCUS       DASUCJ010000843         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1247109, whole genome shotgun sequence.
ACCESSION   DASUCJ010000843 DASUCJ010000000
VERSION     DASUCJ010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247109"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFU90_10060"
     CDS             <1..597
                     /locus_tag="VFU90_10060"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480166.1"
                     /translation="PNLDELADISVMFATEPNHGDEFWPYLRDPETLARPWAIPGTPG
                     LMHRVGGIEKEDGTGNISYDPENHDKMTRLRAAKIAGIANDIPAVVVDDPDGADHLVV
                     SWGSTWGAAKAAVRRIRARDLEIAHVHLHHLHPFPRNLGEVLHRYRAILVPELNLGQL
                     SRLLRAEYLVDAHSYTRIAGIPFRAAELEQQILEMMNR"
     gene            594..>1311
                     /locus_tag="VFU90_10065"
     CDS             594..>1311
                     /locus_tag="VFU90_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU5480167.1"
                     /translation="MTDAPVKLARKDFQSDQEVRWCPGCGDYGILAAIQFMLPDTGIK
                     PEELVFVSGIGCAARLPYYMDTYGVHGIHGRAPAIATGVALARPDLHVWVIGGDGDML
                     SIGGNHLIHALRRNVNLKILLFNNQIYGLTKGQYSPTSELGKITKSTPMGSLDYPFNP
                     VALALGAEATFVARSIDRDRAHFAEVLRAAARHEGAAFVEIYQNCNVFNDGAFEGITG
                     KEVRADMLIELKHGEPIRFGA"
BASE COUNT          239 a          449 c          420 g          203 t
ORIGIN      
        1 ccgaacctcg acgaactcgc cgacatctcg gtgatgttcg cgaccgaacc gaaccacggc
       61 gacgagttct ggccgtactt gcgggatccg gaaacgttgg cgcggccgtg ggcgatcccg
      121 ggcacgcccg gcctgatgca ccgtgtcggc ggcatcgaaa aagaagacgg caccggcaac
      181 atcagttacg accccgagaa ccacgacaag atgacgcgat tgcgcgccgc aaaaatcgcg
      241 ggcatcgcga acgacatccc ggccgttgtc gtcgacgatc ccgacggcgc cgaccacctc
      301 gtggtttcgt gggggagcac gtggggtgcg gccaaagcgg cggtgcggcg catccgtgcg
      361 cgcgacctcg agatcgcgca cgtgcacttg caccatctgc atccgttccc gcgcaacctg
      421 ggcgaggtgt tgcaccgcta ccgcgcgatc ctcgttcccg agctcaacct gggccagctg
      481 tcgcgactct tgcgcgccga gtacctcgtc gacgcgcaca gctacacgcg aattgcgggc
      541 attccgttcc gcgcggcaga gctcgaacag cagatcttgg agatgatgaa ccgatgaccg
      601 acgcgcccgt gaaactggcc cgcaaggact tccagtcgga tcaggaagtc cgctggtgcc
      661 ccggttgcgg cgactacggc atcctcgccg caatccaatt catgcttccc gacaccggga
      721 tcaagcccga ggagctcgtg ttcgtgtcgg ggatcggctg tgccgcgcgg ctgccgtatt
      781 acatggacac ctacggcgtg cacggcatcc acggccgcgc gccggcgatc gcgacgggtg
      841 tcgcgctcgc gcgtcccgac ctgcacgtgt gggtgatcgg cggcgacggc gacatgctct
      901 cgatcggtgg caaccacctc atccacgcgc tgcgccggaa cgtcaacctc aagatcctgc
      961 tcttcaacaa ccagatctac ggcctgacga agggccagta ctcgccgacc agcgagctcg
     1021 ggaagatcac gaagtcgacg ccgatgggat cgctcgacta tccgttcaac ccggtcgcgc
     1081 tggcgctggg cgcggaggcg acgttcgtcg cgcgctcgat cgaccgcgac cgcgcgcact
     1141 tcgccgaggt gctgcgcgcc gccgcccggc acgagggcgc ggcgttcgtc gagatctacc
     1201 agaactgcaa cgtcttcaac gacggcgcat tcgaggggat caccggcaag gaagtgcgcg
     1261 ccgacatgct catcgagctc aagcacggcg aaccgattcg gttcggcgcc g
//
LOCUS       DASUCJ010000844         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_604795, whole genome shotgun sequence.
ACCESSION   DASUCJ010000844 DASUCJ010000000
VERSION     DASUCJ010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604795"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFU90_10070"
     CDS             complement(<1..323)
                     /locus_tag="VFU90_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5480168.1"
                     /translation="MHRAIDLRGAVAVNVITMIGIGPLVTMPLVIAALGGPLALVGWI
                     AGAIVALCDGLVWAELASRFPGSGGTYVYLRRAFGADSVGRALAFLFNWQFLLYAPCL
                     LASGY"
     gene            377..1417
                     /locus_tag="VFU90_10075"
     CDS             377..1417
                     /locus_tag="VFU90_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5480169.1"
                     /translation="MHERSDLARLMQLRGDVRVDFVESEALRGNALGDPARRPVAVYL
                     PPQYDELGSRRFPVLYVLHGYTGNAAALLASRPWETNVFEWIDRLIAQERMAPALLAV
                     VDGFTRLGGSQYVDSVHNGDYATYTVRDVVGHVDSRYRTIAAEGGRAVLGKSSGGFGS
                     MHLAMEHPGVFAAFASHSGDSYFRYAHPPAFVHVQRTLEQHDGNVAAFVAAFEGRRKR
                     SQADYLTMEMLGYAAAYSPRSSRAYDLELPFDARTGALDDAVFDRWLSFDPAERVASR
                     VRELGRLRLRYLDCGRRDEYALDIGARVTAQRMRDHGLEVRHEEFDDDHRNVGYRYEI
                     SLPALAAVLDAE"
     gene            1414..1998
                     /locus_tag="VFU90_10080"
     CDS             1414..1998
                     /locus_tag="VFU90_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480170.1"
                     /translation="MKRLAFFALTLALALAPAPARLRAQIPPPPTPSPDPHTYSDPAM
                     NFTAPPDAVLVGRRVIPVNQLGQDLQMVALWVLHPGRDDARTIQLAMESFEGPPDQWE
                     AQFESQMHSAGSSDLLVRRKQSMSLLNGMPASFVEITSGSGFTSKKEFAVVWADGQRG
                     IALSYTARLGDASSVDALAALKNATAVRYPIEQP"
     gene            complement(1964..>2637)
                     /locus_tag="VFU90_10085"
     CDS             complement(1964..>2637)
                     /locus_tag="VFU90_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU5480171.1"
                     /translation="DNTFATPYFQRPLALGADVVVHSTTKYIGGHSDTVGGVAIVKDR
                     ALHDVVKFHQNATGGVPGPHDAWLTMRGAKTLAIRMQEHARNAQIVAEFLEAHDGVAR
                     VYYPGLPSHPQHELAKRQMSGFGGMLSFVLEGAPERALDFAHRLRYFSLAESLGGVES
                     LICHPARMTHGSIPAEQRQRRGVTDGLLRLSVGIEDAGDLVDDLDNALAATEVTAARS
                     GTVPR"
BASE COUNT          447 a          876 c          886 g          428 t
ORIGIN      
        1 atgtagccgc tcgccaacaa gcacggggca tagagcagga actgccagtt gaacaggaaa
       61 gccagggcgc gcccgacgga atcggccccg aacgcgcgac gcaggtagac gtacgtcccg
      121 cccgatcccg gaaagcgcga cgcgagctcg gcccagacca ggccgtcgca cagcgcgacg
      181 atcgcgcccg cgatccagcc gacgagggcc agcggtccgc cgagcgccgc gatgacgagc
      241 ggcatcgtga ccagcggtcc gatgccgatc atcgtgatca cgtttaccgc gacggcgccg
      301 cgcaaatcga tcgcacgatg catggtggcc gaccttcggt gaggtgaagg ccgtacctgg
      361 cgacgaatca ccgcatatgc acgaacgttc ggatcttgcc aggctcatgc aactgcgcgg
      421 cgacgtacgc gtcgatttcg tcgaaagcga ggcgttgcgg gggaacgcgt tgggcgaccc
      481 tgcgcggcgc ccggtggcgg tctacctgcc gccgcagtac gacgaactcg gttcgcgccg
      541 ctttcccgtg ctctacgtgc tgcacgggta caccggcaac gcggccgcgc tgctcgcgtc
      601 gcggccgtgg gaaaccaacg tgttcgagtg gatcgaccgg ctgatcgccc aggagcggat
      661 ggcgcccgct ctcctggcgg tggtcgacgg ttttacccgc ctcggcggat cgcagtacgt
      721 cgattcggtt cataacgggg actacgcgac gtacacggtc cgcgacgtcg tggggcacgt
      781 cgattcccgc tatcgcacga tcgcggccga gggcggccgc gccgtgctgg gcaaatcgtc
      841 cggcggtttc ggctcgatgc acttggcgat ggaacatccc ggagtgtttg ccgcgttcgc
      901 gtcgcacagc ggcgattcgt attttcgtta cgcccatccg ccggcgttcg tgcacgtcca
      961 gcgcacgctg gagcagcacg acggcaacgt cgcagctttc gtcgccgcct tcgaaggccg
     1021 tcgcaaacgc agtcaagccg actacctgac gatggagatg ctcggctacg ccgccgccta
     1081 ttcgccccgc agcagccgtg cgtacgatct cgagctgccg ttcgacgcgc gcaccggcgc
     1141 actcgacgat gcggtcttcg accggtggct gagcttcgac ccggccgaac gagtggcatc
     1201 gcgggtccgc gaactagggc gattgcgctt gcggtatctc gactgcggcc gccgcgacga
     1261 atacgcgctc gacatcggcg cgcgcgtcac cgcgcagcga atgcgcgacc acgggctgga
     1321 ggtccgtcac gaggaattcg acgacgatca ccgcaacgtg ggctaccgct acgaaatctc
     1381 gctgccggcg ctggcggcgg tcctggacgc cgaatgaaac gactcgcctt tttcgctttg
     1441 accctggcgc tggcgctcgc acccgcgccg gcgcgattgc gcgcgcagat tccacctccc
     1501 ccgacgccgt cgccggatcc gcacacctac tcggacccgg ccatgaactt cacggcgccg
     1561 ccggacgcgg tgctggtggg ccggcgcgta ataccggtca accaactcgg ccaagatctg
     1621 cagatggtcg cgctgtgggt cctgcatccg ggccgggacg acgcgcgcac cattcaactg
     1681 gcgatggaat ccttcgaagg tccgccggat cagtgggaag cgcagttcga aagccaaatg
     1741 cacagcgcgg gcagcagcga tctgttggta cgccgcaagc aatcgatgtc gttgctcaac
     1801 ggcatgccgg ccagcttcgt ggagataacc tccggaagcg gtttcacgtc gaaaaaggag
     1861 ttcgcggtgg tgtgggccga cggtcagcgc ggaatcgcgc tctcgtatac cgcccggctc
     1921 ggcgacgcga gctccgtcga cgcgctcgcg gcgctgaaga acgctaccgc ggtacggtac
     1981 ccgatcgagc agccgtaact tcggtagcgg ccaaagcgtt gtcgaggtcg tcgacgaggt
     2041 cgccggcatc ctcgattcca accgaaaggc gcagcaagcc gtcggtaact ccccggcgct
     2101 ggcgctgttc ggccgggatc gagccgtggg tcattcgtgc cgggtggcag atcagcgact
     2161 cgacgccgcc gaggctttcg gccaagctga aatagcgcag ccggtgcgcg aaatcgaggg
     2221 cgcgctccgg cgccccctcg agtacgaacg acagcatgcc gccaaaaccg ctcatctgcc
     2281 gttttgcgag ctcgtgctgc gggtgggacg ggagccccgg gtagtagacg cgcgcgacgc
     2341 cgtcgtgcgc ttccaagaat tcggcgacga tttgggcgtt gcgggcgtgc tcttgcatcc
     2401 gaatcgccag cgtcttcgca ccgcgcatcg tcagccacgc atcgtgcgga cccggaacgc
     2461 cgccggtggc gttctggtgg aacttcacga cgtcgtgcag ggcgcggtct ttcacgatcg
     2521 cgaccccgcc gaccgtgtcg ctgtggccgc cgatgtactt ggtggtggag tgaaccacga
     2581 cgtcggcgcc gagcgcgagt ggccgctgaa aatacggcgt cgcgaaggtg ttgtcga
//
LOCUS       DASUCJ010000845         1168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_804017, whole genome shotgun sequence.
ACCESSION   DASUCJ010000845 DASUCJ010000000
VERSION     DASUCJ010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804017"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFU90_10090"
     CDS             <1..451
                     /locus_tag="VFU90_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5480172.1"
                     /translation="PLPNGATAIGDALQLAARNLPAHGHRVVILITDGVNNAGADPLE
                     AAQWLGAHRIPVYTVGIGTPSGGLIPGTNDVATIDEDALRQYASLSGGAYARAENATQ
                     LRDALARLGRLTSLERQRVNASLGFALAGAAGMLIAFVAGFGLGRYP"
     gene            448..1038
                     /gene="pdxH"
                     /locus_tag="VFU90_10095"
     CDS             448..1038
                     /gene="pdxH"
                     /locus_tag="VFU90_10095"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU5480173.1"
                     /translation="MNALARSQLDPNPFAQLQRWIADAAAAGIGEPSRMCLATVGPGG
                     GPSARMVLLRGLDDRGLRFYTSYFSRKGRELSENPAVAAVLYWPELERQVRVEGTAAQ
                     LSDEESDEYFATRPRGHRLAAWASEQSEPIEERDILEQRFAHFADRFDDDVPRPHSWG
                     GYLIAPARFAPKEEFAAVGFFGRIQGRHRTGHPRKI"
BASE COUNT          188 a          381 c          413 g          186 t
ORIGIN      
        1 tccgctgccc aacggcgcga ccgcaatcgg cgacgcgctg caactggccg cgcggaattt
       61 gccggcccac ggccaccgcg tcgtcatctt gatcaccgac ggcgtgaata acgccggcgc
      121 cgatcccctc gaagcggcgc agtggctggg cgcgcaccgc attcccgtct atacggtcgg
      181 catcgggacc ccgtcgggcg gactgattcc gggaacgaac gacgttgcga cgatcgacga
      241 agacgcgctg cggcagtacg cgagcctgag cggcggcgcc tacgcgcggg cggagaacgc
      301 gacccaactg cgcgacgcgc tcgcgcggtt ggggcggttg acctcactcg agcggcaacg
      361 cgtcaacgcc tcgctgggct ttgctttagc gggcgcggcc ggaatgctga tcgcgttcgt
      421 ggccggcttc ggattgggac ggtatccgtg aacgcgctgg cccgttcgca gctcgatccg
      481 aatccgttcg cgcagctgca acgatggatc gccgacgccg ccgcggccgg catcggcgaa
      541 ccgagccgca tgtgcttggc aaccgtcggc ccgggcggcg ggccctccgc gcgtatggtg
      601 ctgctgcgcg ggctcgacga ccgcggtctg cgtttctaca cctcgtactt cagccgtaaa
      661 gggcgggagt tgagcgagaa ccccgccgtc gcggcagtgc tctactggcc ggaactcgag
      721 cgccaagtgc gcgtcgaagg cacggccgct cagttatcgg acgaagagtc ggacgagtat
      781 tttgcgacgc ggccgcgcgg gcaccggctg gcggcgtggg cgtcggagca gagcgaaccg
      841 atcgaagagc gggacatcct ggagcaacgg ttcgcgcact tcgccgacag gttcgacgac
      901 gacgtgcccc ggccgcactc gtggggaggt tatctgatag caccggcgcg attcgcgcca
      961 aaggaggagt tcgccgccgt cgggtttttc ggtcggattc aaggccgtca tcgtacgggg
     1021 cacccgcgga aaatttgagc ggttgaaagc ggcttcggga aacctcgcac gctcccttgg
     1081 agcggcgcct acggctggag tcgacgcacc tgccaactgc tccctgagag cgtatattcg
     1141 aaccggtcgt gcatgcggtc ggggcgtc
//
LOCUS       DASUCJ010000846         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_849279, whole genome shotgun sequence.
ACCESSION   DASUCJ010000846 DASUCJ010000000
VERSION     DASUCJ010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849279"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..859)
                     /locus_tag="VFU90_10100"
     CDS             complement(188..859)
                     /locus_tag="VFU90_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5480174.1"
                     /translation="MIDDSSSATPRREFLGKVAAASIALGLGGALPQRLDAAIDAAAP
                     DVEKWPDVHGKHRQMYDAVSWNHGLSLVWAMIFLDTNKASSNLDDKDLSAVVVMRHEG
                     VVLAFTDPIWKKYKLGEAFKIDDPATKAPAERNPFFHAKDGELMFPGMDIAKLMDRGV
                     TFGACNVALTVYSGMRADALGIDKDTAKKEWTAGLIPGFTLLPSGVWGVNRAQEHGCS
                     YCYAS"
     gene            complement(935..1741)
                     /locus_tag="VFU90_10105"
     CDS             complement(935..1741)
                     /locus_tag="VFU90_10105"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5480175.1"
                     /translation="MEKSAQAAPTPPAGDVVPMRPPSDSDIPSGALGVSIRRGHALLT
                     ATRDSLPAYAPSGLRCVSCHLDDGRRAHASPFVGVYARYPVYNARSGKAYTIEDRIND
                     CFRRSLNGRALPMGSSDMRDIVVYFAWLSRGVPVGAAVEGQGLAKLTPLVGDTTRGRA
                     LFGAQCARCHGATGAGTTVAPPLWGKASFNIGAGMSRLRTAAAFIRYNMPFDRPGSLD
                     DQQAFDVALYITSRPRPDFAGKENDWPNGGAPPDVAYRTRAAASKDTARR"
     gene            complement(1966..2127)
                     /locus_tag="VFU90_10110"
     CDS             complement(1966..2127)
                     /locus_tag="VFU90_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480176.1"
                     /translation="MIRDLDLLRPIYYPTAAYGHFGREPGPQGTFSWESPSRASALQD
                     EARVVSAIS"
BASE COUNT          382 a          686 c          712 g          353 t
ORIGIN      
        1 gcgcttcgca tgatcgagca gatgtgctca cccagataga cgtgccgcga gatgcatacc
       61 gcgcgcatga tgcggcaata tagtgccgac cgtcgcgaca atcgtccgcg ttgtgaaaca
      121 gatgcgacgc cgatctcgac aaccgacggg gcgggcgcga acgcccgccc gtcggtcggg
      181 aattacgcta cgatgcgtag cagtagctgc agccgtgttc ctgcgcgcga ttcacgcccc
      241 acacgccgct cggcagcaag gtgaagccgg gaatgaggcc ggccgtccac tccttcttcg
      301 cggtgtcctt gtcgataccg agggcgtcgg cgcgcatccc gctgtaaacg gtgagtgcga
      361 cgttgcaggc gccgaacgtc acgccgcggt ccatcagttt cgcgatgtcc atccccggga
      421 acatcagctc gccgtccttg gcgtggaaga acggattgcg ctcggccggc gccttggtgg
      481 ccggatcgtc gatcttgaac gcctcgccga gcttgtactt cttccagatc ggatcggtga
      541 aggcaagcac aactccctcg tggcgcatca ccacaaccgc gctcaggtct ttgtcgtcca
      601 gattgctcga ggccttgttg gtatcgagga agatcattgc ccagacgagg gagaggccgt
      661 ggttccagct cacggcatcg tacatctggc gatgtttgcc gtgaacatcc ggccactttt
      721 cgacgtccgg cgcagccgca tcgatggcgg cgtccagccg ttggggcaag gcgccgccca
      781 agcctaacgc aatggacgcg gccgcaacct tgcccaggaa ttcgcgtcgg ggcgtggccg
      841 aggatgagtc gtcgatcatg tgctgcctcc tgagtcgtgt tgaagtcccg agaagcggtg
      901 gacggcaggt actggctacg gcaatcatgg accgctagcg tcgtgcggtg tccttcgatg
      961 cggcggcacg cgtccggtat gcgacatcgg gcggagcacc cccgttaggc caatcgtttt
     1021 ccttgccggc aaagtcgggt cggggacgcg acgtgatgta caacgcgacg tcgaaagcct
     1081 gctgatcgtc gagcgatccc ggccggtcga acggcatgtt gtaccggatg aacgccgccg
     1141 ccgttcgcag gcggctcatc ccggctccga tgttgaacga cgccttgccc cagaggggcg
     1201 gcgcgaccgt cgtgccggct cccgtggctc cgtggcaacg cgcgcactgg gcgccgaaca
     1261 gcgcgcggcc gcgggtcgta tcgccgacga gcggcgtgag cttcgcgagc ccctgccctt
     1321 cgacggctgc gccgaccgga acgccgcgcg acaaccaggc gaagtagacg acgatgtcgc
     1381 gcatgtccga gctgcccatc ggcagtgcgc gtccgttcag gctgcgacga aagcagtcgt
     1441 tgatccgatc ttcgatcgtg tacgccttac cgctccgcgc attgtagacc ggatagcgcg
     1501 catagacgcc gacgaatggc gatgcgtgcg cgcgccggcc gtcgtcgaga tgacagctca
     1561 cgcagcgcag gccgcttggc gcgtacgccg gcaggctgtc gcgcgtggca gtgagcagcg
     1621 cgtggccgcg ccggatggag acgcctaacg caccagacgg aatgtcgctg tcgctgggcg
     1681 ggcgcatggg gacgacatcg ccggcggggg gagtcggcgc cgcctgggcg ctcttttcca
     1741 cggccggcgg agacgatccg cgacacgcca ggagcgatgc caccaacacc acaacgcaac
     1801 cggcgtgcag atgacatgga cggaggagga tgggaaggcg catgcgacag ggctggcggg
     1861 tacgtcacgg atttcccccg ccacgccgcg tgagcaatcc gtgacaaacg tactgccgat
     1921 cctagcgcgc ccagagaaga aacgccagag catttcccgt gcaggtcaac tgattgcaga
     1981 taccacgcgc gcctcgtcct gcagcgcact cgcacgcgac ggcgactccc aactgaacgt
     2041 tccctgcggg cctggctccc ggccgaaatg gccgtatgcg gccgtcggat aataaatggg
     2101 ccgcaacaga tcgagatcgc ggatgatggc gta
//
LOCUS       DASUCJ010000847         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_238133, whole genome shotgun sequence.
ACCESSION   DASUCJ010000847 DASUCJ010000000
VERSION     DASUCJ010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238133"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFU90_10115"
     CDS             complement(<1..211)
                     /locus_tag="VFU90_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5480177.1"
                     /translation="MARIYENLADAVGNTPLVKIPRLNAGLGGTVLVKMESFNPAGSV
                     KDRIGVSMIEAAERDGRLRPGSIIIE"
     gene            306..>1094
                     /locus_tag="VFU90_10120"
     CDS             306..>1094
                     /locus_tag="VFU90_10120"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5480178.1"
                     /translation="MRSFQYVAVATAAAIAFPATVAAQSSRPSLDDAVAAIAAYAPAA
                     MAYQGTPGLALAITGRTKTIRILTFGLADVDAKTPVTPQSRFAVGSITKSMTALALMQ
                     LYDAKKLSLDAPVTRYLPWFSIDSSGVPIRVHELLSHTAGLPDDFSLENGYVYDLVAL
                     RGAHVLFKPGTSWSYSNDGFSAAGAILSRLDGRPWYAALQARVLDPLAMSDSAPAFTP
                     ETLATAAVGYQWRDNDRPGSLHPALVESPSMDFVDPAGSVLATPE"
BASE COUNT          171 a          379 c          347 g          197 t
ORIGIN      
        1 gctcgatgat gatcgaacca ggacgcagcc ggccgtcgcg ttcggcagcc tcgatcatag
       61 agacgccgat gcgatccttg accgagccgg ccggattgaa cgactccatc ttcaccaaca
      121 cggtgcctcc gagtccggca ttgaggcgcg ggattttgac caggggggtg ttgccgacgg
      181 cgtcggccag attttcgtaa atgcgtgcca tggtagagcc cttaaccgct cgaaacgccc
      241 gagcgtttca ccgctgacgg gaacgcctgc ggggcggcgg cgggagcgcc gagtatctac
      301 gccccatgcg ctcttttcag tacgttgcgg ttgcgacggc agccgcaatc gccttccccg
      361 cgacggtcgc cgcgcaaagc tcgcggccgt cgctcgacga cgcggtcgcg gccatcgcgg
      421 cgtacgcgcc cgcggcgatg gcgtaccagg gtacgcccgg gctcgcgctt gcgatcaccg
      481 gccgcaccaa aacgattcgc atcttaacct tcggcctcgc cgacgtcgac gctaagacgc
      541 cggtcacgcc gcagtcgcgc tttgccgtcg gctcgatcac caagtcgatg acggcgctgg
      601 ccttgatgca gctctacgat gcgaaaaaac tctcgctcga cgcgcccgtc acccgctatc
      661 tgccgtggtt ctcgatcgac tcgagcgggg ttcccattcg cgtgcacgag ctgctgtcgc
      721 acaccgccgg gcttcccgac gatttctcgc tggaaaatgg atacgtgtac gatctggtcg
      781 cgctgcgcgg cgcgcacgtg ctcttcaaac ccgggacgag ctggtcgtat tccaacgacg
      841 gcttttcggc cgccggagcg atcttgtcgc ggctcgacgg ccgtccgtgg tacgccgcgc
      901 tgcaggcgcg cgtgctcgac ccgctcgcca tgagcgacag cgcgccggcg ttcactcccg
      961 agacgctcgc gacggcagcc gtcggctacc agtggcgcga caacgatcgt ccgggttcgc
     1021 tacatcccgc gctggtggaa tcgccgtcga tggatttcgt cgatccggcc ggttcggtgc
     1081 tcgcaacccc cgag
//
LOCUS       DASUCJ010000848         1074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_987182, whole genome shotgun sequence.
ACCESSION   DASUCJ010000848 DASUCJ010000000
VERSION     DASUCJ010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987182"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..>1074)
                     /locus_tag="VFU90_10125"
     CDS             complement(350..>1074)
                     /locus_tag="VFU90_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480179.1"
                     /translation="LPLPFEHAKIAYAAIVAALAIAAVAARKAFVARTVPPIVTIAAC
                     AFVIFAAGTYAAGVHVLDRHAASLYLPVTLSAFAAIALLKPPASVRAAAVWTCVAVAA
                     SLIALGVTYRALAKPGDWARATAYVRAHERPGEPIAVFEAENALPFAYYYDGPNRIVP
                     IPEGVDFRRYDVSSFVVRDDAQLAAAMPRTARLWLVTAGECKASNIAFGCGLVEGYAV
                     RRYRVLSDESFFGSRVRLVERL"
BASE COUNT          213 a          353 c          355 g          153 t
ORIGIN      
        1 ggcgtgtcgc cggtgtggat cggccctccg ggagtatcgg ccggcgcaag cacgtgcgcg
       61 accgacgcgt tgtgtatcgg ggcgtgcgga ttagacgcga atgcgagtat gagtgcgagg
      121 agaaatgcca cgtttctcta cccttctccg gagctgcaaa ccagaaggta cgtagttaaa
      181 aatgaccagg tacccccaac cttactcctt atcgggagat tatgctagca tcgagagtcc
      241 aaaattttgg gggaagttgg tcaccggggg ggtgggacca cgataatatc tactcctgcg
      301 cacacagtcg ggcccggccg aagccaagag gattcctcga gccgcccggc tagagccgct
      361 ctaccagccg gacccgcgac ccaaagaacg actcgtccga aagcacccgg taccgccgca
      421 cggcgtatcc ctccacgagg ccgcacccga aggcgatgtt cgaggccttg cactcgccgg
      481 ccgtaaccag ccataggcgc gccgttcgcg gcatcgccgc cgccagctgg gcgtcgtcgc
      541 gcaccacgaa gctcgaaacg tcgtagcgcc ggaagtcgac cccctccggg atcggcacga
      601 tgcgattggg gccgtcgtaa tagtaagcga acggcaacgc gttttcggct tcgaagaccg
      661 cgatcggctc gcccgggcgc tcgtgggcgc gaacgtaggc ggtagcacgt gcccaatccc
      721 ccggcttggc gagcgcgcga tacgtaacgc ccagcgcgat cagcgaggcg gcgacggcga
      781 cgcacgtcca caccgccgcg gcgcggacgc tcgccggcgg cttcagcagc gcgatcgccg
      841 caaacgcgct cagcgtaacc ggcaagtaca gcgaagcggc gtgccggtcg agcacgtgca
      901 cgccggccgc atacgtcccc gcggcgaaaa ttacgaaagc gcaagcggcg atcgtcacga
      961 tcggcgggac ggtacgtgcg acgaacgctt tgcgcgcggc cacggccgcg atggcgagcg
     1021 ccgcaacgat cgcggcatac gcgatcttcg cgtgctcgaa gggcagcggc aaga
//
LOCUS       DASUCJ010000849         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_849309, whole genome shotgun sequence.
ACCESSION   DASUCJ010000849 DASUCJ010000000
VERSION     DASUCJ010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849309"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /locus_tag="VFU90_10130"
     CDS             complement(<1..2250)
                     /locus_tag="VFU90_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU5480180.1"
                     /translation="MSHITRQLRLLCTIALTAAALAPTAVRAQQAIDSAYTAQIKQDL
                     EDPRISTELVDYLPASATVPSPLKVLGHIIGAPGILDRSADIYKYFDALAAASPRVKV
                     WRIGKTEEGRDMILAAVADEQTIKELDKYRGMLAALGDPRKTTPEQAQQLIHTAKPIY
                     YITSGIHSPEFGGPEMLMELAYRLAVDSSQFIQNIRNNVIVLITPVIEPDGRDKAVDT
                     YYYNKKQPKGAPRLPLMYWGRYVQHDNNRDGMGQFLQLTKHTTQAVLDWHPTILHDLH
                     EAQTYLYASTGTGPYNEQIDPITIDEWWMLAENDVMEMTKRGVPGVWTYGFYDGWTPN
                     YLFFIAHTHNELGRFYEVQSYGPDNYVVKPRPTTTSREWFRPNPPLDSINWGPRNNTN
                     IQESAILFALSDVAKNKETFLDNYWRKNVRAVDKGKTGPVYGWVIPAQQYRKANAADA
                     VNDLRAQGLEVHTANAAFTAGGVSVKQGDYIIRGDQPYRTLADMYFALQHFSPKNPTP
                     YDDTGWTFPLMRDITITPIGDKRLLDQPMTILTKDAVAPGGVSGAGGVVVVDNTADNN
                     IAAFRFKFAKTRMDAAEQDFDADGHHFRAGALIIPNANRTQLDGALQRLGLSAYAMAS
                     VPHVKTHPLDVPRIGYVHSWTRTQDEGWVRAALDTYGIPYTYFGDVKLRDGNLRRKYD
                     VIIMPHIGGTVQSMVNGIPKRGATPLPYKKTAEFQSFGTPDSSSDIRGGMGLDGVRNL
                     ETFVEQGGTL"
     gene            complement(2366..2542)
                     /locus_tag="VFU90_10135"
     CDS             complement(2366..2542)
                     /locus_tag="VFU90_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480181.1"
                     /translation="MLWRVSARGGTARAASTLPFEPDRCYCALSSDGKRWVGAVTHPI
                     SDIFLLRGFDADLR"
     gene            2638..>2838
                     /locus_tag="VFU90_10140"
     CDS             2638..>2838
                     /locus_tag="VFU90_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480182.1"
                     /translation="MMRSGVFARASTTRSPAACSGLTYSGVPRLAPVSVRRLAASWTA
                     SAIPKSASTASPSCRRMLSGLMS"
BASE COUNT          429 a          888 c          948 g          573 t
ORIGIN      
        1 cagcgtcccg ccctgctcga cgaacgtctc caaattcctg acgccgtcga gtcccatgcc
       61 gccgcggatg tcgctcgacg aatcgggcgt gccgaagctc tggaactcgg cggttttctt
      121 gtagggcagg ggcgtggcgc cgcgcttcgg gatgccgttc accatgctct gcaccgtgcc
      181 gccgatgtgc ggcatgatga tcacatcgta tttccggcgc agatttccat cgcggagctt
      241 cacgtcgccg aagtacgtat acgggatgcc gtaggtatcg agcgcggcgc gcacccaccc
      301 ctcatcctgc gtacgcgtcc aactgtggac gtagccgatg cgcggaacat ccagcgggtg
      361 cgtcttgacg tgaggcacag atgccatcgc gtacgccgag aggccgagtc gctggagcgc
      421 gccgtcgagc tgcgtgcgat tggcgttcgg gatgatgagt gcgccggcgc ggaagtgatg
      481 gccgtcggcg tcgaaatcct gctcggcggc atccatcctg gttttcgcga acttgaatcg
      541 gaacgccgcg atgttgttgt ccgccgtgtt gtccaccacg acgacgccgc cggcgccgct
      601 cacgccgccg ggcgccaccg cgtccttcgt cagtatcgtc atcggctgat cgagcagacg
      661 cttgtcgccg atcggcgtga tggtgatgtc gcgcatgagc gggaaggtcc accccgtgtc
      721 atcgtacggc gtgggatttt tgggcgagaa gtgctgcagc gcgaagtaca tgtcggcgag
      781 cgtgcggtag ggttggtcgc cgcgaatgat gtagtcgccc tgcttcaccg agacgccgcc
      841 ggccgtgaag gcggcattcg cggtgtgcac ttcgagtccc tgcgcgcgca ggtcgttcac
      901 cgcgtccgcg gcgttcgcct tacgatattg ttgggcgggg atcacccatc catacaccgg
      961 accggtcttc cccttgtcca ccgcgcggac gttcttccgc cagtagttgt cgaggaacgt
     1021 ctccttgttc ttcgcgacgt cggagagcgc gaacaggatg gcggattcct gaatgttggt
     1081 gttgttgcgc ggcccccagt tgatgctgtc caacggcggg ttgggccgga accattcgcg
     1141 gctcgtggtc gtcggccgcg gtttcacgac gtagttgtcc ggcccgtagc tctggacttc
     1201 gtagaaccgg ccgagctcgt tgtgggtgtg ggcgatgaag aagagatagt taggcgtcca
     1261 gccgtcgtag aaaccgtacg tccacacgcc cggcaccccg cgcttggtca tctccatcac
     1321 gtcgttctcg gcgagcatcc accattcatc gatcgtgatc gggtcgatct gctcgttgta
     1381 cggaccggtg ccggtggacg cgtagagata cgtctgcgct tcgtgcaggt cgtgcagaat
     1441 cgtcgggtgc cagtcgagca ccgcctgcgt ggtgtgcttg gtgagctgga ggaactgccc
     1501 catcccgtcg cggttgttgt cgtgctgcac gtacctgccc cagtacatca gcggaagccg
     1561 cggggcgcct ttcggctgct tcttgttgta gtagtaggtg tcgaccgctt tgtcgcgtcc
     1621 atccggttcg atcaccggcg tgatcaacac gatcacgttg ttgcggatgt tctggatgaa
     1681 ctgcgacgaa tcgacggcca ggcgataggc gagctccatg agcatctccg gtccaccgaa
     1741 ctccggcgaa tggatgccgc tcgtgatgta atagatcggc ttcgcggtgt ggatgagctg
     1801 ctgcgcctgt tccggagtgg tcttgcgcgg gtcgcctaac gcagccagca tgccgcggta
     1861 tttgtccagc tccttgatcg tttgctcgtc ggccaccgcc gcgaggatca tgtcccggcc
     1921 ttcttcagtc ttgccgatgc gccacacttt cacgcgcggc gacgcggcgg cgagcgcgtc
     1981 gaagtacttg tagatgtccg cgctgcggtc gaggatgccc ggcgcgccga tgatgtggcc
     2041 caacaccttg agcggcgacg gcacggtcgc cgacgcgggc aaatagtcga cgagctcggt
     2101 cgatatgcgc ggatcctcga ggtcctgctt gatctgcgcc gtgtacgccg agtcgatcgc
     2161 ctgttgggcg cggactgccg tgggcgcgag cgctgccgcg gtgagcgcga tggtgcagag
     2221 cagccggagc tgccgcgtga tgtgggacat cctcatgttc ctctcgagag gggatcgagc
     2281 cgccggtaga tcgggttcgg ggcggcctga ggggccgccg gaagtagtac gcgatgggaa
     2341 cgcaggacgt tgaggcgcgt cctgcctaac gtaggtcggc gtcgaacccg cgcaagagaa
     2401 aaatgtcgct gatcgggtgc gtcaccgctc ccacccatcg cttgccgtcg ctcgacaggg
     2461 cgcagtagca gcggtcgggc tcgaacggca gcgtgctcgc ggccctggcc gttcctccgc
     2521 gcgccgacac ccgccacagc atgggccgat gcggccgccg ccaacgcgcc gaaataaatc
     2581 gacccgtcgg acatccaggc gaacggatcc agtacttcgt tgccgaactc gaaccaggtg
     2641 atgcgctctg gcgtcttcgc gcgcgcgtcc accacgcgat cgccggccgc ttgctccgga
     2701 ctcacgtact ccggcgtgcc caggctcgcg ccggtctccg ttaggcgtct cgccgcctcc
     2761 tggaccgcga gtgcgatccc gaagtccgcg agtaccgctt cgccttcgtg caggaggatg
     2821 ttgtcgggct tgatgtcc
//
LOCUS       DASUCJ010000850         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1094234, whole genome shotgun sequence.
ACCESSION   DASUCJ010000850 DASUCJ010000000
VERSION     DASUCJ010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094234"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFU90_10145"
     CDS             <1..389
                     /locus_tag="VFU90_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480183.1"
                     /translation="TISLNNGATTISMDDGGRASIESYGRNIPISAGQTVQLGDGASV
                     SCNQDGSLSVDACNAQGGRISTTLAAQGKGVNVDVTAHDVDLGGALVRDERLQPDPLQ
                     TPLPILDPVTAAPQGPERLYPDGYVE"
     gene            581..1192
                     /locus_tag="VFU90_10150"
     CDS             581..1192
                     /locus_tag="VFU90_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480184.1"
                     /translation="MRTAVEVVAIVAAGLWALYTFVYEQRIKPLSEAPSFSVPTTVDQ
                     GPTVDGVAFLTIHKRLDNTGNVGVDIAAEAFSVYGEKLDAHAGVRGVRTPVRAELSAD
                     VERRPVKVLYAVAKLRYGAVGGSPTDFFVPPHSSGVEDYLVAVPVRDYPVVLISRKDF
                     IEKSPIAPAIPVRIVKSPLGGYDLASPVLQGEFDSQVEYPIRP"
     gene            1259..>2089
                     /locus_tag="VFU90_10155"
     CDS             1259..>2089
                     /locus_tag="VFU90_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HEU5480185.1"
                     /translation="MNIAPFFACLLALTPALRTVDASAAVSPAELRMVVVVSRHGVRS
                     PTHPNELQAYAKDPWPAWSGHPGNLTTRGALLMRQAGEYYRSFYGPLLGMSAGACPPP
                     GSVFVWADVDQRTRTTGAAITQGFAQGCALAVHHATGDADPLFDPLKGSGGVNRAASR
                     ASILGAVGSNFAALNAAYEPAFVTLERVLGCAAPAACKQIATVPTTVANDGDGGLATI
                     SGGLAMAGDVAENILLEYVDGRSDVGWGRVDHSTLLQLLQFQVLGKKLEHGNRYTARA
                     H"
BASE COUNT          368 a          690 c          674 g          357 t
ORIGIN      
        1 cgacgatttc gctgaataac ggtgcgacga cgatctcgat ggacgacggc ggacgcgctt
       61 cgatcgaaag ctacggccgc aatatcccga tctcagccgg acaaacggtg cagctcgggg
      121 acggcgcgag cgtcagctgc aatcaggacg gatcgttgag cgtcgacgcg tgcaacgctc
      181 aaggcggacg aatctcgacg acgctcgcgg cccagggtaa gggcgtcaac gtagatgtga
      241 ccgctcacga cgtggatctc ggcggcgcgc tggtgcgcga cgagcgcttg cagcccgacc
      301 cgcttcagac tccgctgccg atcctggacc cggtcaccgc cgcgccccaa ggcccggaac
      361 ggctctaccc ggacggctac gttgaataag gaagagcgcg cccgtcgcag ccggggcaag
      421 cgaacgcgat cgtgttgcgc ccgggccgca agtcgagcat ctccctagag ccacgagcag
      481 atggcggtgt agaagtctgg ggtcggcgcc cgtcgaacta ggcggagctt ttatgcctcg
      541 agaattgcac gtccactcgg tcccgtcggc gggcgaaaac ttgcgaacgg cggtcgaagt
      601 cgtcgcgatc gtcgccgccg ggctttgggc gctctacacc ttcgtctacg aacagcgcat
      661 caagccgctg tcggaagccc cgtcgttttc ggtgccgacg accgtcgatc aggggccgac
      721 cgtggacgga gtcgcttttc tcaccatcca caagcggctc gacaataccg gcaacgtcgg
      781 cgtcgacatc gccgccgaag cgttcagcgt gtacggtgag aagctcgacg cgcacgccgg
      841 cgttcggggt gtccgcacgc cggttcgagc cgagttgagt gccgacgtcg aacggcggcc
      901 ggtgaaggtg ctctatgcgg tcgcaaagtt acgctacggt gcggtcggcg gcagcccgac
      961 ggacttcttc gtgccgccgc atagctccgg agtggaggac tatctggtcg ccgttccggt
     1021 gcgggattac ccagtcgtct taatctcccg caaagacttc atcgagaaga gtccgatcgc
     1081 tcccgcaatt ccggttcgga tcgtgaaatc gccgctgggc ggatacgatt tggcgtcgcc
     1141 ggtactgcag ggcgaattcg attcgcaagt cgagtatccg attcggcctt gacgtcatgt
     1201 agttgtcacg ttgcaccgct agaatcgcaa cgacgctctg ctgaaaggat tgccgctcgt
     1261 gaacatcgcc ccgttttttg cgtgcctgct cgctcttaca cccgcgttac gcaccgtcga
     1321 cgcgagtgcc gcggtttcgc cggcggagtt gcggatggtc gtcgtcgtca gccggcacgg
     1381 cgtgcgctcg ccgacccacc caaacgaact gcaggcctac gcaaaagacc cctggccggc
     1441 atggagcggc catccgggca acctcaccac gcgcggcgcc ctgctgatgc gacaagcggg
     1501 agagtactat cgaagcttct acggtccgct gctgggcatg agcgccggcg cttgtccgcc
     1561 gccgggctcg gtcttcgtat gggccgacgt cgatcagcgc acgcgcacca ccggagccgc
     1621 gatcacccag gggttcgcgc aagggtgcgc cctcgccgtg catcacgcga ccggcgacgc
     1681 cgatcccctc ttcgatccgc tgaagggcag cggtggcgtc aaccgcgcgg catcgcgcgc
     1741 gtcgatcctc ggcgccgtcg gttctaactt cgccgcgctc aacgccgcct acgagcccgc
     1801 tttcgtcacg ctcgaacgcg tgctcggatg cgccgcgccg gccgcctgca aacagatcgc
     1861 gacggtgccg acgaccgtcg caaacgacgg cgacggcggt ctggcgacga tctccggagg
     1921 cctggctatg gccggcgacg tcgccgaaaa tatcctgctg gaatacgtcg acggacgttc
     1981 cgacgtcggc tggggacgcg tcgaccacag taccttatta cagctgctgc agttccaagt
     2041 tctcggcaag aaactcgagc acggaaaccg ctacacggcg cgcgcgcat
//
LOCUS       DASUCJ010000851         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_39498, whole genome shotgun sequence.
ACCESSION   DASUCJ010000851 DASUCJ010000000
VERSION     DASUCJ010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39498"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFU90_10160"
     CDS             complement(<1..329)
                     /locus_tag="VFU90_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480186.1"
                     /translation="MKPAFRRRFGVFAVALACAALVCGSAAPAAVVMSTGIRYVIPSG
                     SAGDCSTKAQTALGTYLDAPVESSTGSGDWLAIGPKGRTGPSSSAAAVRCYPVGKGYV
                     VTFTCVV"
     gene            complement(326..772)
                     /locus_tag="VFU90_10165"
     CDS             complement(326..772)
                     /locus_tag="VFU90_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480187.1"
                     /translation="MVGTLYFRAACFAVALALSFLLVCRPAPADLGMVIGPRVVVTNQ
                     PVSDCSAKAKAALNSVLQNAFEAGDGTNQWLAYGPLDSTGHSSATAAIHCYPVDSGYV
                     ATLTCAAQVPPNPDTATTLCVKLAAAFPAQNAALIDRPGSVAGSAP"
     gene            complement(877..1068)
                     /locus_tag="VFU90_10170"
     CDS             complement(877..1068)
                     /locus_tag="VFU90_10170"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEU5480188.1"
                     /translation="MARKVIDCRDYPSERNCTVTIAADTADELLEVAVLHATHAHGHE
                     DTPELRRQLRDGVKEAAAT"
     gene            complement(1106..>1288)
                     /locus_tag="VFU90_10175"
     CDS             complement(1106..>1288)
                     /locus_tag="VFU90_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480189.1"
                     /translation="LDVLRPLPGYKIDHVDIEFVPYGHAGYAIPHYDVHAYYVAHPVH
                     VAYCPNGETELRKPAQ"
BASE COUNT          211 a          417 c          435 g          225 t
ORIGIN      
        1 tcgacgacgc acgtgaacgt cacgacgtaa cctttgccca cggggtagca gcgtaccgct
       61 gccgccgagc tggaaggccc ggtgcgccct ttcgggccga tcgccagcca gtcgccgctg
      121 ccggtcgacg attcgaccgg agcgtcgaga tacgtgccca gcgcggtttg cgctttggta
      181 ctgcagtcgc cggcggagcc gctcggaatg acgtaacgaa tgccggtcga catcaccacc
      241 gcggcaggtg cggccgagcc gcacacgagc gccgcgcacg ccagcgcgac ggcaaacacg
      301 ccaaagcggc gtcgaaacgc cggcttcacg gtgcgcttcc tgcgaccgaa ccgggccggt
      361 cgatcagtgc ggcgttttgg gcgggaaacg ccgccgccag cttgacgcac agcgtcgtcg
      421 ccgtatccgg gttgggcggt acctgcgccg cacaggtcag cgtcgcaacg taaccgctat
      481 cgaccggata gcagtggatg gcggccgtgg ccgaggagtg acccgtgcta tcgagcggtc
      541 cgtacgcgag ccactgattg gttccgtcgc cggcttcgaa ggcgttttgc agcaccgagt
      601 tgagcgcggc tttggctttg gcgctgcagt cgctcaccgg ttggttggtc acgacgacgc
      661 gcgggccgat caccatcccg agatcggcgg gggcgggccg gcagaccagc agaaaactca
      721 gcgcgagtgc aaccgcaaag caggcggcgc gaaaatacag tgtcccgacc acgtaacggc
      781 cctcctaccc cgaaggcgtt ttgacgaccc cgaggctcac tcccctaggg gtacgggctg
      841 ctaacggctg cggcgacggt cctgtgacgg ccgttcttac gtggccgcgg cttccttgac
      901 gccgtcgcgc aactgccggc gcagctccgg cgtgtcttcg tggccgtgcg cgtgcgttgc
      961 atgcaggacg gccacttcga gcagttcgtc cgccgtgtcg gcggcgatcg tcaccgtgca
     1021 gtttcgttcg ctcggatagt cgcgacaatc gatgacttta cgtgccatga caattctccg
     1081 ttcgccgcgc gtgcgcgcgg cagttttatt gagccggttt cctgagctcc gtttcgccgt
     1141 tgggacagta cgcgacgtgc accggatgcg ccacgtagta cgcgtggacg tcgtagtgag
     1201 gaatcgcata gccggcgtgc ccgtacggaa cgaactcgat gtcgacgtga tcgatcttgt
     1261 atcccggcag cgggcgcagg acgtcgag
//
LOCUS       DASUCJ010000852         1375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_329663, whole genome shotgun sequence.
ACCESSION   DASUCJ010000852 DASUCJ010000000
VERSION     DASUCJ010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329663"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFU90_10180"
     CDS             complement(<1..1323)
                     /locus_tag="VFU90_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480190.1"
                     /translation="MNGEERVYGRGDDFAEVQVDPTNADEIYVANTSTYRSTDGGHRF
                     TAIKGAPGGDDYHRIWINPDNPKIILIGSDQGATITVNGGQTWSSWYNQPTGQMFHVA
                     ADNRFPYRVYGGQQESGSAGVSSRGNDGAITFRDWHPVGVEEYGYAVPDPLHAGIIFG
                     GKVTRYDETTGQVQDVGPVVLRGGTYRFDRTAPIIFSPADPHVLYFAAQVLFKTTNGG
                     RSWSTISPDLTRPDPGVPASLGVYAQQAAQHPHRGVIYSIAPSGRDANLIWVGTDDGL
                     IQVTHDGGAHWTNVTPSGMEAWSKVTQIDASHFDSVSAYASASRLRVDDLHPHIYRTH
                     DGGRTWQSIMTGIPADEPVNVVREDPVRRGLLYAGTEHTVYVSFDDGAHWQSLRLDLP
                     ITSIRDLIVHDNDLVIATHGRSFWVLDDIAPLRQLDARLASSAAFLFKP"
BASE COUNT          223 a          443 c          457 g          252 t
ORIGIN      
        1 gggcttgaac aggaacgcgg ccgacgatgc caagcgcgca tcgagctgcc gaagcggcgc
       61 gatgtcgtcg agcacccaga acgagcggcc gtgcgtcgcg atcacgagat cgttgtcgtg
      121 cacgatgaga tcgcgaatcg atgtgatcgg caagtcgagc cgaagcgact gccagtgcgc
      181 gccatcgtcg aacgacacgt acaccgtgtg ctccgtgccg gcgtacaaca atccgcggcg
      241 cacgggatcc tcgcgcacca cgttcaccgg ctcgtcggcc gggatgccgg tcatgatcga
      301 ctgccacgtc ctgccgccgt cgtgcgtgcg gtagatgtgg gggtgcaggt cgtccacgcg
      361 caggcgcgac gccgacgcgt acgccgacac gctgtcgaag tgtgacgcat cgatctgcgt
      421 gactttgctc cacgcttcca tcccggacgg cgtgacgttg gtccagtgcg cgccgccatc
      481 gtgcgtcacc tgaatcaggc cgtcgtccgt acccacccag atgagattgg cgtcgcgccc
      541 cgatggcgcg atcgaataga tcacgccgcg gtgggggtgc tgtgccgctt gttgggcata
      601 gacgccgagg ctcgccggca cgcccggatc cggacgcgtg agatcggggc tgatcgtgct
      661 ccagctgcgg cctccgttag tcgtcttgaa cagcacctgc gccgcgaagt acaacacatg
      721 cggatccgcg ggcgaaaaaa tgattggcgc ggtgcgatcg aaccggtagg tgccgccgcg
      781 cagcacgacg ggtcccacat cctgtacctg ccccgtggtc tcgtcgtagc gcgtcacctt
      841 gccgccgaag atgatgccgg cgtgcagcgg atcgggaacg gcgtatccgt attcttcgac
      901 gccgaccgga tgccaatcgc ggaacgtgat cgcgccgtcg ttgccgcggc tcgagacgcc
      961 ggcggacccg ctctcctgct gtccgccgta cacgcgatac ggaaatcgat tgtcggcggc
     1021 gacgtgaaac atctgcccgg tgggctgatt gtaccacgag ctccacgttt ggccgccgtt
     1081 cacggtgatc gtcgcgcctt gatcgctgcc gatgaggatg atcttcgggt tgtcgggatt
     1141 gatccagatg cggtgatagt cgtcgccgcc gggcgcgccc ttgatcgcgg tgaagcggtg
     1201 accgccgtcg gtggagcgat atgtggaggt gttcgcgacg tagatctcgt ctgcgttagt
     1261 cggatccacc tgcacttcgg cgaagtcatc gccgcgcccg tagacccgtt cttcgccatt
     1321 cacgcgcgtc caggtttcgc cggcgtcgtc ggagcggtac acgccgccca acttc
//
LOCUS       DASUCJ010000853         1350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_513509, whole genome shotgun sequence.
ACCESSION   DASUCJ010000853 DASUCJ010000000
VERSION     DASUCJ010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513509"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFU90_10185"
     CDS             <1..900
                     /locus_tag="VFU90_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEU5480191.1"
                     /translation="TKLLITFSMMLFGGMPHGHGLGGWTGLNAVIGHLANGDNSYGWR
                     FLDVVFGALVVMLLYAFAKRITGSTIFASLTALLLTLDGMHFVQSRIATPEGIVIFFA
                     TLATYAFYRFWISSQAVERPHVEAPAWAFAAAAGAALVAAIVAASIGRAIWKFDGPTT
                     VIVALYLACGFYLLLRYTGLTRLFGDGRRELTFGEGSVALQSADGTGTVYAADGGTID
                     DRGKISRGVRSQNRGGALVYADGPLSIEYRRDASVVYETPAATATYAQDEVRVPERGS
                     ENGRASKMWLVLFTVALGCLVSS"
     gene            913..>1350
                     /locus_tag="VFU90_10190"
     CDS             913..>1350
                     /locus_tag="VFU90_10190"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480192.1"
                     /translation="MGFGVSFIVLIAVFLQRYALRLRPALWGNPRGFRLDGALVTIVF
                     VSVTVYGLVWAPDLLRHSTDPNEIHNLNDVVARQAEMFHYHDTLKATHPYSSKWWEWP
                     LDLVPVAYFYQDHRINQSDSSGCCIYEITSMPNPAILWFGLLCV"
BASE COUNT          201 a          421 c          464 g          264 t
ORIGIN      
        1 accaaactgc tgatcacgtt ttcgatgatg ctgttcggcg ggatgccgca cggccacggg
       61 ctgggcggtt ggacgggcct caacgccgtc atcggacacc tcgccaacgg cgacaactcc
      121 tacggctggc ggtttttgga cgtcgtcttc ggggcgctgg tggtgatgct gctgtacgcg
      181 ttcgccaagc gcatcaccgg atcgacgatc ttcgcgagcc tgaccgcgct gctgctgacg
      241 ctcgacggga tgcatttcgt gcagtcgcgg atcgcgacgc cggagggcat cgtgatcttc
      301 ttcgcgacgc tggccacgta cgcgttctac cggttctgga tttcttcgca ggcggtcgaa
      361 cggccgcacg tcgaggcgcc ggcgtgggcg ttcgcggcgg cggccggcgc ggcgctggtc
      421 gcggcgatcg tcgccgcgtc gatcggacgc gcgatttgga agttcgacgg gcccaccacg
      481 gtcatcgtcg cgctatatct cgcctgcggt ttctatctct tgctgcgcta taccgggctg
      541 acgcggctgt tcggcgacgg ccggcgcgaa ctaaccttcg gcgagggatc ggtcgcgctg
      601 caatcggccg acggcaccgg cacggtgtac gccgccgacg gcggcacgat cgacgatcgc
      661 ggaaagattt cgcgcggcgt gcgctcgcaa aaccggggcg gtgcgctggt ttacgccgac
      721 ggccccttaa gcatcgaata ccggcgcgac gccagcgtcg tgtacgaaac gccggcggca
      781 accgccacct acgcgcagga cgaggtgcgc gtgccggagc gcggcagcga gaacgggcgc
      841 gcgtcgaaga tgtggctggt gctgttcacc gtcgcgctgg gctgtctggt gagcagctag
      901 tggtaaggcg tgatgggctt cggcgtgagc ttcatcgtgc tgatcgcggt cttcttgcag
      961 cgctacgcgc tgcgtctgcg cccggcgtta tggggcaacc cgcgcggctt ccggctggac
     1021 ggcgcgctgg tgacgatcgt gttcgtcagc gtcaccgtgt acgggctggt gtgggcgccg
     1081 gatctgctgc ggcattcgac cgatcccaac gagatccaca acctcaacga cgtggtcgcg
     1141 cggcaggccg agatgtttca ctatcacgac actctcaaag ccacgcatcc gtactcctcg
     1201 aagtggtggg agtggccgtt ggatctcgtg ccggtggcgt atttctacca ggatcatcgc
     1261 atcaatcaaa gcgactcctc gggctgctgc atctacgaaa tcacgtcgat gcccaatccg
     1321 gcgattctct ggttcgggct gctgtgcgtg
//
LOCUS       DASUCJ010000854         1821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_161824, whole genome shotgun sequence.
ACCESSION   DASUCJ010000854 DASUCJ010000000
VERSION     DASUCJ010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161824"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFU90_10195"
     CDS             complement(<1..694)
                     /locus_tag="VFU90_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480193.1"
                     /translation="MERFARAAAAIAARSGKLEKIAVLADYLRELGDADLIAATRFFT
                     GRAFAARDSRTLSIGGRSIVKVARALWNFDDAALGEAYRSTGDLGSALAQLVRPPAAP
                     MLLPEPPLTPARLDDLFGEIGDAVGKNAAKRRESALEQILRACREPAVAAYVVKIVTG
                     DLRLGLREGLVLDAIAHAFAAEPAAVRRAVMASGDVGAAALAARDQRLDDVRVEYGTP
                     IGFMLASPIAYGQ"
     gene            complement(701..>1821)
                     /locus_tag="VFU90_10200"
     CDS             complement(701..>1821)
                     /locus_tag="VFU90_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5480194.1"
                     /translation="DPLAMSDSAPAFTPEALATAAVGYQWRDNDRPGSLHPALVESPS
                     MDFVDPAGSVLATPEDMARYMRLYLNGGRTAGGTPLISPQAFAKMTDADMRSDGKPAG
                     SAGVELPEAPEYYRRYGFGLSIFDQNGDRLIGHTGGISGYTACMQMNLTRGFGVIAMA
                     NLVEAPLHPCAIMLYAMRVLRAQSLGEPLPLPPPAPDPAQVTRAGDYAGTYAAPGAAT
                     LTVQAEGDHLAMLDGGTKIALYPRGADQFWADDPRFSLYLIAFGRDADKRVVELTYGS
                     QWYPNQRYAGPRAFPHPKAWDALVGRYENVYWGQPDVTRVVIVKDKLTVDGVAPLRPL
                     RQGAFALGDSVVRFDAFAGAFPQRMSIDDARFYRTELP"
BASE COUNT          297 a          604 c          639 g          281 t
ORIGIN      
        1 tctgcccgta agcgatcggc gacgccagca tgaagccgat cggcgttccg tactccacgc
       61 gcacgtcgtc gagccgttgg tcgcgggcgg caagtgccgc cgcgccgacg tcgcccgacg
      121 ccatcacggc ccgccgcacg gccgcgggtt cggcggcaaa cgcgtgcgcg atcgcgtcga
      181 ggaccaggcc ttcgcgaagt cctaaccgca gatcgccggt gacgattttt acgacgtacg
      241 cggcgactgc gggttcgcgg caggcgcgca ggatttgttc gagcgcggat tcgcggcgtt
      301 tggcggcgtt tttgcccacg gcgtcgccga tttcgccgaa cagatcgtcg agccgagcgg
      361 gcgtcagcgg cggttcgggc aacagcatcg gcgcggcggg cggccggacg agttgggcca
      421 gcgcgctgcc caagtcgccg gtcgaacggt acgcttcgcc gagcgccgcg tcgtcgaagt
      481 tccagagggc gcgcgccacc ttgacgatgc tgcgtccgcc gatcgagagc gtgcgcgaat
      541 cgcgcgcggc gaacgcgcgt ccggtgaaga agcgggtcgc ggcgatcaga tccgcgtcgc
      601 cgagctcgcg caggtaatcc gcgagcaccg cgatcttctc gagttttccg ctgcgagcgg
      661 caatcgccgc ggcagcgcgc gcgaaccgct ccatgcgggg ttacggcagc tcggtgcgat
      721 agaagcgcgc atcgtcgatc gacatccgct gcggaaacgc gccggcaaaa gcgtcgaagc
      781 ggacgaccga atcgcccagt gcgaaggccc cctgccgcag cggccgcagc ggcgcgaccc
      841 cgtcgaccgt gagcttatcc tttacgatta cgacccgcgt gacgtcgggc tggccccaat
      901 agacgttttc gtagcggccc accagcgcgt cccaggcctt aggatgcggg aaggctctcg
      961 gcccggcata gcgctggttc ggataccatt gcgagccgta ggtgagttcg acgacccgct
     1021 tgtcggcgtc gcgtccgaac gcgatcaagt atagcgaaaa gcgcgggtcg tcggcccaaa
     1081 actgatcggc gccgcgtggg tacagcgcga ttttggtgcc gccgtcgagc atcgcgagat
     1141 ggtcgccttc ggcctggacc gtaagcgtgg cggcaccggg cgccgcgtac gttccggcgt
     1201 agtcgccggc acgagtcacc tgcgcgggat cgggagccgg cggcggcaac ggcagcggtt
     1261 cacccagact ttgcgcgcgc agcacccgca tcgcatagag catgatcgcg cacgggtgca
     1321 gcggcgcttc gaccaaattc gccatcgcga tcacgccgaa gccgcgcgtg aggttcatct
     1381 gcatgcacgc ggtgtaaccg gagataccgc cggtgtgacc gatcagccga tcgccgttct
     1441 ggtcgaaaat cgagaggccg aagccgtaac ggcgatagta ctccggcgcc tcgggcagtt
     1501 cgacgccggc cgatccggcc ggctttccat cgctgcgcat gtcggcgtcg gtcattttgg
     1561 caaacgcctg cggcgagatc aacggcgtgc cgccggcggt tcggccgccg ttgagataca
     1621 agcgcatgta gcgcgccatg tcctcggggg ttgcgagcac cgaaccggcc ggatcgacga
     1681 aatccatcga cggcgattcg accagcgcgg gatgcagcga acccggacga tcgttgtcgc
     1741 gccactggta cccgacggct gccgtcgcga gcgcctcggg agtgaacgcc ggcgcgctgt
     1801 cgctcatggc gagcgggtcg a
//
LOCUS       DASUCJ010000855         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1033209, whole genome shotgun sequence.
ACCESSION   DASUCJ010000855 DASUCJ010000000
VERSION     DASUCJ010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033209"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(575..>1083)
                     /locus_tag="VFU90_10205"
     CDS             complement(575..>1083)
                     /locus_tag="VFU90_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480195.1"
                     /translation="RKVIGLYDATITATDPFPDREAFAGPDPTLAGISPAYTTAINRQ
                     LRSEIGVETDREYTVLSHEVNELWRNDAQQHFFTPPIGAADDFRYGMSLNPHMKAFIT
                     HGRYDLVTPYYATDRLRNLMRLDAETAERLTVRHFAGGHMFYAWEESRREFAGAIATF
                     VAHAVATA"
BASE COUNT          167 a          377 c          362 g          177 t
ORIGIN      
        1 tgccgccacg cctgaacgcg gccccgggac gccgcacaat cgcgccgggc acccacctcg
       61 cacaacatcc cgtgccacgg acattgcgcg caccccccga gccggcgccg tcgacgcgtc
      121 ccgcccggtt tcggatcaat ctgacaactc tcgccagacg cgctgtccag cttacgtctt
      181 tgtcgcacac gcggccgtgg acgccgcgct tccgcttcga gtgccgatcg gtgagaagga
      241 gcagcgtcgc gtcggcacca tcgccgcgtc ggacagctcc ctgttcgtga aagcaggtct
      301 ctcgctgcgg cacgctcgat gagcgggtcg tgctgtccgc gcacacgcac accgcggacc
      361 cggaccgcgc tcgacccaga gcattgtctg ccgtcgccgg ggcgattctg aaatcgacgg
      421 acgccgcgcc gagcgcgatc gcccgagggc actactttaa agccggtcgc cgcgaccgca
      481 tccgaggtct gaccgcgatg ccggccgtgt gagcggcgct cgcaaggctg gcaacgctca
      541 gcgcacgatc cggacgtggc gcacgtgccg aggactacgc tgtcgcgacc gcgtgggcga
      601 cgaacgtggc gatcgccccg gcgaactcgc gccggctttc ctcccacgcg tagaacatgt
      661 ggccgccggc gaagtggcgg acggtgagcc gctcggcggt ctccgcgtcg agccgcatga
      721 ggttccgcag ccggtcggtc gcgtagtacg gcgtcacgag gtcgtagcgc ccgtgcgtga
      781 tgaacgcctt catgtgcgga ttgagcgaca tgccgtagcg gaagtcgtcg gcggcgccaa
      841 tgggaggtgt gaagaagtgc tgctgcgcgt cgttgcgcca caactcgttc acctcgtggc
      901 tcagcaccgt gtactcgcga tcggtctcga cgccgatctc cgatcggagc tgccggttga
      961 tcgccgtcgt gtacgcggga ctgatgccgg cgagcgtcgg gtcgggaccg gcgaacgcct
     1021 cccgatcggg aaacggatcg gtggctgtga tcgttgcgtc atacaacccg atcactttcc
     1081 gtt
//
LOCUS       DASUCJ010000856         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_895641, whole genome shotgun sequence.
ACCESSION   DASUCJ010000856 DASUCJ010000000
VERSION     DASUCJ010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895641"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFU90_10210"
     CDS             <1..961
                     /locus_tag="VFU90_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480196.1"
                     /translation="AIPNSELQPKGKNAPMTLRFSLAVAAMFVALGAASGAARAQEAS
                     PAPSPAPVAAPEATEHPNFMDQQYDGRLHINAAPYVWAPTVKSNYQFTIPVLPRAVGG
                     VTQGSVQVPPVDYASKLNTAAMLAFSARMGQIMVLGDYIYLNASTSASVAGAITGPLG
                     RRAIPYTIDTNARLATSIWELAAGFAIAHGHNADLNVFAGWRQFPSSVTFDYNATVGK
                     RGIIAPAGTIVSKETQTDAIFGIQGKAYFSDDHWYIPYYADYGVGATNQSWQAYGGAG
                     YTFNHGQSIVVLYRTLNYSGFPQGDVIQKLSMGGPLIGYTFPI"
     gene            1155..>2001
                     /locus_tag="VFU90_10215"
     CDS             1155..>2001
                     /locus_tag="VFU90_10215"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480197.1"
                     /translation="MKRLFAALILTLLLVAPQPGGATGSATPFQQLTSMLHWRSIGPF
                     IGGRVVAVAGVPGKPNVFYFGAVQGGVWTSDDYGQHWRNLTDGKIPGIASPIGAIAVA
                     PSNPNVIYAGTGEADIRGDFASGDGIYKSTDAGKTWSYAGLRDTHMTAKLLVDPRDPN
                     VVYAASMGHVWAPNADRGVYKSTDGGKTWRKVLFVDSNTGGVDLAMVPRSPNVVYAAM
                     WQAQRLPWKLTSGGPGSGLFKTSDGGAHWRKISTNPGFARALLGRIGVSVAASDPRVV
                     YAIVQA"
BASE COUNT          373 a          652 c          629 g          347 t
ORIGIN      
        1 tgcgattccg aactcagagc tccagccgaa gggaaaaaac gcacccatga cgttacgatt
       61 cagcctcgcg gtcgccgcca tgtttgtggc actcggtgcg gcgtccggag cggcgcgcgc
      121 gcaagaagcc tcgccggcgc cttcgccggc acccgtcgcg gcgccggagg ccaccgagca
      181 cccaaacttt atggatcagc agtacgacgg ccgcctgcac atcaacgcgg cgccgtacgt
      241 ttgggctccc accgtgaagt cgaactatca gtttacgatt ccggtcttgc cgcgcgccgt
      301 cggcggggtc acgcagggca gcgtgcaggt tccgccggtc gactacgcct cgaaactgaa
      361 caccgccgcg atgctggcct tcagcgcgcg gatggggcag atcatggtgc tgggcgacta
      421 catttatctc aacgcctcga cgagcgcgtc ggtggcgggc gcgatcaccg gtccgctggg
      481 acgccgggcg attccgtata ccatcgatac gaacgcgcgc ctcgcgacgt cgatctggga
      541 gctcgccgcc ggattcgcga tcgcacacgg ccacaacgcc gacctcaacg tgtttgcggg
      601 ctggcggcag tttccgtcga gcgtgacgtt cgattacaac gcgaccgtcg gcaagcgcgg
      661 catcatcgcg cccgcgggga cgatcgtctc gaaagaaacg cagacggacg ccatcttcgg
      721 catccaaggc aaagcgtatt tcagcgacga ccactggtat attccttact acgccgacta
      781 cggagtcggt gccacgaacc aatcgtggca agcctacggc ggtgccggat atacattcaa
      841 tcacggtcag tcgatcgtcg tgctgtaccg aacgctgaac tattcgggat tcccccaagg
      901 cgacgttatt cagaaactct cgatgggcgg tccgctgatc gggtatacct tcccgatctg
      961 acgcgtccgg ccggcacggg agcgcgcggg tctggtgacc cgctcgaagc acgtgaacgc
     1021 cagtggaagc atattccgct ggcgtttgcg cgcaacgccg gctatctgat gcacgtctga
     1081 tgcagcgcgc acgtagacgc gtcgatcgcg gctgaaggag gacattctcg acgctcttgc
     1141 gcgatactcc gactatgaag cgcctctttg ccgccctcat tctgacgctg ttgctcgtcg
     1201 cgccccaacc cggcggagcg acgggcagcg cgaccccatt ccagcagctc acgagcatgc
     1261 tgcattggcg cagcatcggc cccttcatcg gcggacgcgt cgtcgcggtc gccggcgtgc
     1321 cgggaaagcc gaacgtcttc tacttcggtg cggttcaagg gggcgtttgg acgagcgacg
     1381 attacgggca gcactggagg aaccttactg acggaaaaat ccctggaata gcgagtccca
     1441 tcggcgcaat cgcggtcgct ccttcgaacc cgaacgtcat ctatgccggg acgggcgaag
     1501 ccgacattcg aggcgacttc gcatcgggcg acggtatcta taagagcacc gacgcgggca
     1561 aaacgtggtc gtacgccgga ttgcgcgaca cgcacatgac ggcgaagctc ctcgtcgatc
     1621 cgcgcgatcc caacgtcgtc tacgccgcct cgatggggca cgtttgggca cccaacgccg
     1681 accgcggcgt ctacaagtcg accgacggcg gcaagacgtg gcgtaaggtg cttttcgtcg
     1741 actcgaatac cggaggcgtc gatctcgcga tggttccgcg ctcgccgaac gtcgtttacg
     1801 ccgcaatgtg gcaagcgcag cgacttccct ggaagctcac cagcggaggc cccggcagcg
     1861 gcctcttcaa gacgtccgac ggcggcgcgc attggaggaa gatatcgacc aatcccgggt
     1921 tcgcacgcgc cctgctcggc aggatcggcg tgagcgttgc ggcgagcgat ccgcgcgtcg
     1981 tgtacgccat cgtacaggcc g
//
LOCUS       DASUCJ010000857         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_70493, whole genome shotgun sequence.
ACCESSION   DASUCJ010000857 DASUCJ010000000
VERSION     DASUCJ010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70493"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..544)
                     /locus_tag="VFU90_10220"
     CDS             complement(32..544)
                     /locus_tag="VFU90_10220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5480198.1"
                     /translation="MSVIPDVIEPVSLADYFEVMTRAVFQAGVSWKQIADRWESYRTA
                     FAGFDPRRVAAFDELGVERALATPGILQMRRKVLATVANARTMVELDGDSSAFSAYLR
                     SFESYQALAKDFKKRFSFMGDMNVWYFLFRTGEPVPHFETWVKTIPGDHPRMREMVER
                     ARAAGRSSEC"
     gene            complement(555..>1603)
                     /locus_tag="VFU90_10225"
     CDS             complement(555..>1603)
                     /locus_tag="VFU90_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5480199.1"
                     /translation="KLGYDVHRGIGRTGVVGVVRGERPGRTIMLRADMDALPITEETT
                     HSYRSRIDGAMHACGHDGHVAILLGAAELIAARREELSGTVCLVFQPAEEGAGGARVM
                     LEDGLFERFGIERAYGLHLSSKYGSGVLGFREGPMYASSDSIEITVLGVGGHGSAPHD
                     AVDPIYAAAAFVTSVQQVVSRQVDPLEPAVVTIGSIHGGTIHNVIPRTCRILGTVRAF
                     SEEVRQAMSGRIERILRACCDAAGAGYDYVYLDRYPVTANDPEQTRYAEALARANFED
                     ERVVSAGALMGAEDFSFYALRVPACFYTLGCSRGPDTSHPHHSSLFDLDEACLSTGVA
                     MMTALALDAPRHAP"
BASE COUNT          297 a          509 c          522 g          275 t
ORIGIN      
        1 gcacttgcga tcttcaacgc acgtgccgcg cctagcattc ggaggagcgt cccgcggcgc
       61 gcgcccgctc gaccatctcg cgcatgcggg ggtggtctcc gggaatcgtc tttacccacg
      121 tctcgaagtg cgggaccggc tcgcccgttc gaaaaagaaa gtaccagacg ttcatgtcgc
      181 ccatgaagct gaagcgcttc ttgaaatcct tcgctagagc ctgataggac tcgaacgagc
      241 gcaggtaggc cgaaaatgcg ctcgagtccc catcgagctc gaccatcgtg cgggcgtttg
      301 caacggtagc gagtaccttg cgccgcatct gcaggattcc gggcgtggcc agcgcccgct
      361 ccacgccgag ctcgtcgaac gccgcgacgc gccgcggatc gaatccggca aaagcggttc
      421 gatacgattc ccaacgatcc gcgatttgtt tccagctgac gccggcttgg aacacggccc
      481 gcgtcatgac ttcgaagtag tcggccagcg ataccggctc gatgacgtcg gggatgacgc
      541 tcatatccga tccatcaggg cgcgtgtcgc ggagcgtcaa gcgcgagtgc ggtcatcatc
      601 gcgacgccgg tcgagaggca ggcttcatcg aggtcgaaga ggctcgagtg gtgcggatgg
      661 ctcgtgtccg gcccgcgact gcagccgagc gtgtagaagc acgccggcac tcgaagcgcg
      721 taaaaggaaa aatcttcggc tcccatcagc gcgccggcgg agacgacgcg ctcgtcttcg
      781 aagttcgcgc gcgcaagtgc ttcggcgtag cgcgtttgct cgggatcgtt tgcggtcacc
      841 ggatagcggt cgaggtaaac gtagtcgtag ccggccccgg ctgcgtcgca gcaagcgcgc
      901 aggatacgtt cgatgcggcc ggacatcgct tggcgcactt cctcggagaa ggcccggacg
      961 gtgcccagga tgcgacaggt gcggggaatg acgttgtgga tggtaccgcc gtgaatcgag
     1021 ccgatcgtga cgacggccgg ctccagcgga tcgacctggc gcgacaccac ctgctggacc
     1081 gacgtaacga atgcggcggc ggcgtaaatc gggtcgaccg cgtcgtgggg agcggatccg
     1141 tggccgccga cgccaaggac cgtaatttcg atcgagtcgc tggatgcgta catcggaccc
     1201 tctcggaatc cgagcacgcc gctgccgtac ttggacgaca aatgcaatcc gtaggcacgc
     1261 tcgataccga agcgctcgaa cagaccgtcc tcgagcatga cgcgagcccc gccggcgcct
     1321 tcttcggcgg gctggaacac gagacaaacc gttccgctca gttcctcacg ccgggctgcg
     1381 atgagttcgg cggcaccgag caaaattgcc acgtgtccgt cgtgtccgca tgcgtgcatc
     1441 gcgccgtcga tgcgggaccg gtacgaatgc gtggtttcct cggtaatcgg caacgcgtcc
     1501 atatcggctc gaagcatgat cgtccggccg ggacgttcgc cgcgaacgac gccgacgacc
     1561 ccggtcctgc cgattccccg atgcacgtcg tagccgagct tca
//
LOCUS       DASUCJ010000858         1260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_268951, whole genome shotgun sequence.
ACCESSION   DASUCJ010000858 DASUCJ010000000
VERSION     DASUCJ010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268951"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..671)
                     /locus_tag="VFU90_10230"
     CDS             complement(258..671)
                     /locus_tag="VFU90_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5480200.1"
                     /translation="MADTHYDAVAFDLFGTLVDQTATAMDGAVERLGELRGAAWAIVT
                     SANASLAELLIAHARLPRPPVLVTGDDVERNKPAPEGYALAARRLRVECGRVLASRIR
                     RPAWPRPGPPGWTSSRCSTDAPAILRAPQRGWYGT"
     gene            670..>1260
                     /locus_tag="VFU90_10235"
     CDS             670..>1260
                     /locus_tag="VFU90_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480201.1"
                     /translation="MTSRSFAMLLAACFAAVAACAASLRADAACVGGTVRTTSPGQTP
                     SAAPGLTVPKGFAIFTIARVPHARELIALPDGDLLVGTSSRDVYLVPNAESRTPGTPS
                     VFFTAPDDRAAGVAFSPRRCEIYVATERAVYVASYANGQRAGGTLRKLLDVRTGPIAP
                     HSDGDVHSTTSVAYAETTDSLYVSVGSSCNACVEADP"
BASE COUNT          200 a          459 c          401 g          200 t
ORIGIN      
        1 gcagcgcgcc cgacccgcag gggcgccacg cccttcgcga tcaaatctat tcgatcttga
       61 gtcccgcgca gcaggcgcag cttcacgccg agatgcaggc gcagcgcgcg cggcacatgc
      121 agcggatgga agaacagcag caacagcagc cggtacagcc ggccaacccg cctcgttagc
      181 tacggcccag cgcgctcaaa cgcgcttccc aaactccact tccactgcgc cttcgaccga
      241 acgaaggcgc agtttcgtta ggtcccgtac cacccacgtt gcggcgcgcg caaaatcgcc
      301 ggagcgtccg ttgagcaccg cgacgacgtc catcccggcg gcccgggccg cggccacgcc
      361 ggacgacgta tcctcgacgc cagaacgcgg ccgcattcga ctcgcagccg tcgcgccgcc
      421 aaggcatagc cctcgggcgc cggtttgttg cgctcgacgt cgtctccggt taccaatacc
      481 ggcgggcgcg gcagtcgtgc gtgcgcgatc aggagttcgg ccagcgaggc gtttgccgaa
      541 gtcacgatcg cccaggccgc gccgcgcagt tcccccaagc gttcgacggc tccatccatc
      601 gcggtggccg tctgatcgac cagcgtgcca aagagatcga acgcgacggc gtcgtagtgt
      661 gtgtcggcca tgacctctcg atcgttcgcg atgctgttag ccgcctgctt cgccgcggtc
      721 gcggcctgcg ccgcctccct tcgcgcggat gcagcctgcg tcggtggcac agttcgcacg
      781 acttcgcccg ggcagacgcc gtcggccgcg ccgggactga cggtacctaa gggctttgcg
      841 atcttcacca tcgcgcgcgt gccgcacgcg cgtgagctga tcgcgctacc cgacggcgat
      901 ctcctggtcg gcacgtcgtc gcgcgacgtg tacctcgtgc ccaacgccga gagccggacg
      961 ccgggcacgc cgtcggtatt tttcaccgca cccgacgatc gcgccgccgg ggtggcgttt
     1021 tcgccacgac ggtgcgaaat ctacgtagcc accgaacgcg ccgtgtacgt tgcgagttat
     1081 gcgaacggcc aacgcgccgg cggaacgttg cgcaagctcc tcgacgtgcg taccgggccg
     1141 atcgcgccgc acagcgacgg cgacgtgcac tcgaccactt cggtggccta tgccgagacg
     1201 accgattcgc tgtacgtcag cgtcggttcg tcgtgcaatg cctgcgtcga ggccgatccg
//
LOCUS       DASUCJ010000859         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_55144, whole genome shotgun sequence.
ACCESSION   DASUCJ010000859 DASUCJ010000000
VERSION     DASUCJ010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55144"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1083
                     /locus_tag="VFU90_10240"
     CDS             <1..>1083
                     /locus_tag="VFU90_10240"
                     /inference="COORDINATES: protein motif:HMM:NF039608.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480202.1"
                     /translation="HLQANIHANAQHRYNVGIFMAQDGKTGLLTSANGGSAACSTFGL
                     PIQTSPPSPFADLDGNVCGDFDNSVLASQPPPYPLDLGTVTVTCTPDASGYLDIPTIV
                     TWMTNPNTAGCAAPPAAYFVPESKSKCEAGISQRIQVQVFGKIVVTKATNPAGAAASF
                     SYTASGPSAAPTNFSLSDGQSQTVVTAALGATAQNYVITEQPLAGWTPGAQIVCKDID
                     GDIAPFVDVNNATGTITAHMSVSTDQAFCTFTNTQQASITINKATLGGDGSFNFSGSP
                     NIAPFSITTSGGAGQHSITGLNAGTYSISEAVPAGWDLTGLSCVDPTNDTTVSLANAT
                     ATIALAAGENVTCTYTDTKRASITVVK"
BASE COUNT          215 a          380 c          317 g          171 t
ORIGIN      
        1 catcttcagg ccaacatcca cgctaacgca caacatcgct acaacgtcgg catcttcatg
       61 gcgcaggacg gcaagaccgg cctgttgacg tccgcgaacg gcggcagcgc cgcgtgctcg
      121 acattcggtc tgccgatcca gacgtcgccg ccgtcgccgt tcgccgatct cgacggcaat
      181 gtctgcggcg atttcgacaa cagcgtactg gcgtcgcagc cgccgccgta tccgctcgac
      241 ctcggcacgg ttaccgtcac ctgcacgccg gacgcgagcg gctacctgga catcccgaca
      301 atcgtcacct ggatgacgaa cccgaacacg gcgggctgcg cggctccgcc ggcggcgtat
      361 ttcgtaccgg aaagcaagtc caagtgcgaa gcgggcatct cgcagcgcat ccaggtccag
      421 gtcttcggca agatcgtcgt gaccaaggcg acgaatcccg cgggagcggc cgcatcgttt
      481 tcctataccg cgagcgggcc gtccgccgcg ccgaccaatt tttcgctgtc cgacggtcaa
      541 tcgcagacgg tcgtgaccgc cgcgctcggc gcgacggcgc agaattacgt gatcacggaa
      601 cagccgctcg caggctggac gcccggcgcg cagatcgtct gcaaggacat cgacggcgac
      661 atcgcgccat tcgtcgatgt gaacaacgcg accggcacga tcaccgcgca catgagcgtg
      721 tccaccgatc aggccttttg caccttcacg aacacgcaac aggcctcgat cacgatcaac
      781 aaggccacgc tcggtggcga tggcagcttc aatttcagcg gctcgccgaa catcgcgccg
      841 ttctcgatca cgacgagcgg cggcgccggt cagcacagta tcacgggtct caatgccggc
      901 acgtattcaa tttccgaagc cgtgccggcg ggctgggacc ttactggcct gagctgcgtc
      961 gatccgacca acgacacaac cgtcagcctc gccaatgcaa cggccacgat cgcattggcc
     1021 gcgggcgaga acgtcacctg cacctacacg gacacgaagc gcgcgtcgat caccgtcgtc
     1081 aag
//
LOCUS       DASUCJ010000860         7355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_177106, whole genome shotgun sequence.
ACCESSION   DASUCJ010000860 DASUCJ010000000
VERSION     DASUCJ010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177106"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VFU90_10245"
     CDS             <1..1588
                     /locus_tag="VFU90_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480203.1"
                     /translation="PVFRALQRVAARSAPTRGTFLRAAGLTAVGAAAFGELVACGSSQ
                     ARGRERIAIVGAGIAGLVTAMRLRDAGIGATIYESSARVGGRMHSERTYWSDGQHTEW
                     CGAMVDSMHVNVRGLARRFGLPLLDTIAARPPHARDTCYIDGAYYPMRDADRDFAKIY
                     PIMQMQLGKVNDNTTYANATAEARRLDGMSLHDWIVAYVPGGTASRLGRLIAVAYWNE
                     YGRAPEELSALNLVVQLGEQRRYAQNHEMNVLGYSDQKYIFAGGSQALPEAIARSLPA
                     GSIEFGYRLASIRRAAGGAYELTFSVGGGTQKVTADRVVLAIPFIVLRGLDYSGAGFD
                     AAKTRAIDELGYGYHTKLHLQFDRRAWMTQGRWPEPASGQIWTTLPSQSALDFSLGQP
                     GRDGLLEVFTAGPAAMVDTPPVPYARITDSPAVRRHVGTYFDQLNAIWPGVSRTWNGK
                     ATFGNAQADPNILASYSCWLVGQYTTIAGHEATPQGRVHFAGEHTSVANQGFMEGGAE
                     SGFRAAGEILHDYRVTARGE"
     gene            1592..3445
                     /gene="selB"
                     /locus_tag="VFU90_10250"
     CDS             1592..3445
                     /gene="selB"
                     /locus_tag="VFU90_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5480204.1"
                     /translation="MPIVGTAGHVDHGKSALVAALTGTNPDRWLEEQLRGMTLDLGFA
                     RLAIGDGIEAGVIDVPGHERFLRNMLAGAAGMDVLLLTVAADEGVMPQTREHLDVLRF
                     LNVRETIVVVTKIDLIPHELRAQTQTSIVDALHDTLAAGAPVACVSSLTGEGLDELRG
                     LIAQAVRALPPPASAAPVYLPVDRVFALPGIGTIVTGTLMQGSVAEGDTLALEPSGRR
                     ARVRRIEVFAARQARVEAGSRVALNLAGIDRHDVARGEAVASPEFQARRRFVVRFTPL
                     RDALPLLRKRVAVRAYVGAAEILGTLTFERPPDRAAEVRAELHLQRPALAFPGVRFVV
                     RRLSPKTLLGGGSIEGVATGEDVDARAPAENAVAAVLRSRGLEAVESGDVAFGANLRE
                     DAAREALEALAARDEVLELHRPTAYVDGAAAAELLQRIDAFLEDAQKAEPWALGVTSI
                     ALARALGVPEPLLVRVLTAMTESGRLSGRAGYYATLDHRPSITPEQRAFFDELVPVDA
                     ARPCLPVPYAGVVAAVRRAAVPGAGKAFDTLLARGVLVRVGADLYRGSQIAAIRSRVE
                     VYLRRNHRMTPAQFRDEIGSSRKYAVPLLEWLDSSGFTLRNGDHRTLRRPT"
     gene            3492..6467
                     /locus_tag="VFU90_10255"
     CDS             3492..6467
                     /locus_tag="VFU90_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480205.1"
                     /translation="MHSRFLASAAAASFLLASAAAAAAAAPFGNLAYRSIGPAIAGGR
                     TTAVVGSDRDALLYYAGGADGGVFKSTNGGASWEAVFDRASAASIGAIAIDPRDPGDV
                     WVGTGESNPRNDVESGNGIWHSADGGKTWTHAGLEDAGQISGVAIDPRNPRVVVVGVL
                     GQVFRDSQTRGIYLTRDGGAHWTRTLFAGPSSGISDVVRVPDRPSTLFAGMYEFRRQP
                     WMMTSGGSRGGLYRSDDGGATWRRVSGGGFPAGLTGRIGLAAAGGGRIYAIVQSKLGD
                     LWRSDDGGASWKLMPHNPYVGARPFYFSRIFVDPADRNRLIDVGLILSASKDGGRSFH
                     PIAENAGWDYHVVWWSHDGRRIAVGSDEGAIFSGDAGAHFWQPYDLPFAQAYHAGFGG
                     SLPGYDVCIGLQDNDSWCGPSASYNGIGVLNRDWYTVGPGDGMWALIDPADQDLVWST
                     TTNNDTGQVYLWNRRTQQAYEVSPSARYNSQAPEALPYRFNWDTPLAFASVPSPHVLV
                     GGNVVFESSDRGQHWTIVSPDLTRNDKTHQKASGGPISLDVSGAETSDTILDVETSAL
                     SGTLIWVGTDDGLVQVTRDGGARWSNVTPPGVPPWGRVSTVEPGHFAAGTAYAAIDRH
                     MLGDEHPYVYVTDDYGAGWRSLSANLPSNLFVRSIREDPKDPELLYAGTQRGVWTSFD
                     RGLHWRSLRLNMPATAIYDLEIQPSAGDLIVASHGRGVWILDDLQPLRELARGRVSGV
                     TFFAPRSAYRMFVASPINSFTDGTLPDNEFVGDNPEYGALLSYYLDRPARRRPTLEIS
                     DAHGRIVRHIDGKDVPNDAGIDRTSWDLAEDGPEQWHGTFSENRGPEEGPEVVPGAYT
                     ATLNVDGRAYRQTVDVLADPRDKMPLDDYARRHDFLEEIYAEIGGCNRMLNGIDRKLK
                     QANPQQAQALRAFRAELTFNPRNVEDLGEPPGLRDRLVDAIARSSLSFQPPTAAQLSE
                     AADLKALYANLSQRYEQLK"
     gene            6507..>7355
                     /locus_tag="VFU90_10260"
     CDS             6507..>7355
                     /locus_tag="VFU90_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5480206.1"
                     /translation="MNVDKMTERVGEALNSAYSRALGEHNTQTTPEHMLAALLEQERG
                     IAPDVLAKAGADVPTLERAAADAVARLPRLTGANADNAQVTVSPELARVMTAAENEAK
                     TLSDDYTSVEHVLLAMAQSPGAVGRIFRDAGVTRDKLLQALREVRGNQRVTSKDPEGT
                     YKSLERYGRDLTLEAERGTLDPVIGRDEEIRRIVQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLAQRIVRGDVPEGLKNKRVVSLDMGALIAGAKYRGEFEERLKAVLKDVANSEGQI
                     VLFVDEL"
BASE COUNT         1207 a         2447 c         2520 g         1181 t
ORIGIN      
        1 tcccgttttt cgcgcgttgc agcgagtagc cgctcgcagc gcgccgacgc gaggcacttt
       61 tttgcgcgcc gcgggactca ccgcggtggg cgcggctgcg ttcggcgagc tcgtcgcctg
      121 cgggagttcg caggcgcgcg gacgcgaacg gatcgcgatc gtcggcgcgg gtatcgccgg
      181 gctggtgacc gcgatgcggc tgcgcgatgc cggcatcggc gcgacgattt acgagtcgtc
      241 ggcgagggtc ggcggacgga tgcattcgga gcgcacgtac tggagcgacg ggcagcacac
      301 ggagtggtgc ggcgcgatgg tcgactcgat gcacgtgaac gtccgcgggc tcgcgcgccg
      361 cttcggcctg cccttgctcg atacgattgc agcgcgaccg ccgcatgccc gcgacacctg
      421 ttacatcgac ggcgcttact atcccatgcg cgacgcggac cgcgattttg cgaagatcta
      481 cccgatcatg cagatgcagc tcggaaaggt caacgataac acgacctatg ccaacgccac
      541 cgcggaagcg cgccggctcg acggaatgag tctgcacgac tggatcgttg cgtacgttcc
      601 cggcggaacg gcgtcgcggc tcggccgcct gatcgccgtg gcgtactgga acgaatacgg
      661 acgcgcgccc gaagagctga gcgcgctcaa cctcgtcgtg cagctcggcg agcagcgccg
      721 ctacgcccag aaccacgaaa tgaacgtatt ggggtattcg gaccaaaaat acatctttgc
      781 cggcggaagc caagcgctcc ccgaggcgat cgcccgatcg ctgccggccg gaagcatcga
      841 gtttgggtac cggctcgcga gcatccggcg cgccgccggc ggggcgtacg agctcacgtt
      901 ttcggttgga ggcggcacgc agaaagtgac cgccgatcgc gtcgtgctcg cgatcccgtt
      961 catcgtgctg cgcggtctcg attattccgg cgccgggttc gacgcggcca agacgcgcgc
     1021 gatcgacgag ctgggctacg gctatcacac caagctgcat ctgcagttcg atcggcgcgc
     1081 gtggatgacg cagggccgct ggcccgagcc ggccagcgga cagatctgga cgaccctgcc
     1141 gtcgcaaagc gcgctggatt tttcgctggg tcagccgggc cgcgacggat tgctggaagt
     1201 ctttaccgcg ggtccggcgg ccatggtgga caccccgccg gtgccctacg cgcggattac
     1261 cgattctccg gcggtgcggc gccacgtcgg cacgtatttc gatcagttga acgcgatttg
     1321 gccgggcgtc tcccgaacgt ggaatgggaa agccaccttc ggcaacgccc aagcggatcc
     1381 gaatattctg gcgagctact cgtgctggct cgtcggacaa tacaccacca tcgcgggcca
     1441 cgaagccacg ccgcaagggc gcgtacactt cgccggcgag cacacgtcgg tggcgaatca
     1501 gggcttcatg gaaggtgggg ccgaatccgg atttcgcgcg gccggtgaga ttttgcacga
     1561 ctatcgcgta accgcgcgcg gcgagtaacg cgtgccgatc gtcggcaccg ccggccacgt
     1621 cgatcacggc aaatcggcgc tggtcgccgc tctgaccggg accaatcccg accgctggct
     1681 cgaagagcag ctgcgcggta tgactctcga cctcgggttt gcccggctcg cgatcggcga
     1741 cggcatcgaa gcgggcgtca tcgacgtccc gggccacgag cgtttcttgc gcaacatgct
     1801 ggcgggtgcg gccggtatgg acgtgctgct gctgacggtc gcggccgacg aaggcgtgat
     1861 gccgcagacg cgcgagcacc tcgacgtgct gcgcttcctc aacgtgcgcg agacgatcgt
     1921 cgtcgtcacc aagatcgatc tcattccgca cgagctgcgc gcgcaaactc aaacgagcat
     1981 cgtggacgcg ctgcacgata cgctcgccgc cggcgcgccg gtggcgtgcg tatcgagcct
     2041 gaccggcgaa ggactcgacg agctgcgcgg gctgatcgcg caagccgtgc gcgcgctgcc
     2101 gccgccggcg tcggcggcgc cggtatatct gccggtcgat cgcgtttttg cgctgcccgg
     2161 aatcgggacg atcgtgaccg gaaccctcat gcagggaagc gttgcggaag gcgatacgct
     2221 cgcgctcgaa ccgagcggac ggcgggcgcg cgtacggcgt atcgaggtat tcgcagcgcg
     2281 gcaggcgcgc gtcgaagccg gatcgcgcgt cgccctcaac ctcgccggta tcgatcggca
     2341 cgacgtcgcg cgcggcgaag cggttgccag cccggaattt caggcgcggc gccgtttcgt
     2401 ggtccgcttt acgccgttgc gcgacgcttt gccgctgctg cgaaagcgcg tggcggtgcg
     2461 cgcgtacgtc ggtgccgccg aaatcctcgg cacgctgacg ttcgaacgcc cgcccgatcg
     2521 cgcggccgaa gttcgcgcgg agctgcacct gcagcgaccc gcgctcgctt ttccgggcgt
     2581 gcgtttcgtt gtgcggcgct tgtcgccgaa gacgctgctg ggcggcggct ccatcgaagg
     2641 cgttgcaacc ggcgaggacg tcgacgcgcg cgcgcccgcg gaaaatgcgg tcgccgcggt
     2701 gctccgatcg cgcggcttgg aagcggtcga atccggcgac gtcgccttcg gtgcgaacct
     2761 gcgcgaagat gcggcccgcg aggcgctcga agcgttggcc gctcgcgacg aggtgctcga
     2821 gctgcaccgg ccgacggcgt acgtcgacgg agccgccgcg gccgagctgc tgcagcgcat
     2881 cgacgcgttt ctcgaagacg cgcagaaggc cgagccctgg gctctcggcg ttacgtcgat
     2941 agcgctcgcg cgcgcgctcg gcgttcccga gccgctgctg gtccgcgtgc tgacggctat
     3001 gacggaaagc ggccggctga gcgggcgcgc gggatactat gcgaccctcg atcaccggcc
     3061 gtcgatcacc cccgagcagc gggcgttttt cgacgagctc gtcccggtcg acgccgcgcg
     3121 gccgtgtctc ccggtgccgt acgccggcgt cgtcgccgcg gtgcggcgcg ccgccgttcc
     3181 cggcgcgggc aaggcgttcg acaccctgct cgcgcgcggc gtgctggtgc gcgtcggcgc
     3241 cgatttgtac cgcggctcgc agatcgcggc gatccgctcg cgggtcgaag tctatctgcg
     3301 gcgcaaccat cgcatgaccc cggcgcagtt ccgcgatgaa atcggaagct cgcgaaagta
     3361 cgccgtgccg ctgctcgaat ggctcgactc gagcggtttc acgttgagaa acggcgacca
     3421 tcgtacgctg cgccggccca cgtagcgcgc gagagaggat acgctgcgtt cgcttggtaa
     3481 gcgtcaacgt catgcactcc aggtttctcg cgagcgctgc ggccgcatcg tttctgctgg
     3541 cgagtgcggc ggccgcggcg gcggcggcgc ccttcgggaa cttagcgtat cgttcgatcg
     3601 gcccggcgat tgccggcggg cgcaccaccg cggtggtcgg cagcgatcgc gacgcactgc
     3661 tgtactacgc cggcggcgcg gacggcggcg tcttcaagag caccaacggc ggtgcgtcgt
     3721 gggaggcggt cttcgaccgc gcgtcggcgg cgtcgatcgg cgcgattgcg atcgatccgc
     3781 gcgatcccgg cgacgtttgg gtaggaaccg gcgaatcgaa tccgcgcaac gacgtcgaga
     3841 gcggtaacgg catctggcat tcggccgacg gcgggaagac ctggacgcac gccggtctgg
     3901 aagatgccgg ccaaatctcc ggcgtcgcga tcgacccgcg caatccgcgc gtggtcgtcg
     3961 tcggcgtgct gggccaagtg tttcgcgact cccaaacgcg cggaatctac ctcacgcgcg
     4021 acggcggcgc gcactggacg cggacgctgt ttgcggggcc ctcgagcggc atttccgatg
     4081 tagtgcgcgt tccggatcgc ccgtcgacgc tatttgccgg gatgtacgag ttccggcgac
     4141 agccttggat gatgaccagc ggcggatcgc gcggcggtct ctaccgttcg gacgacggcg
     4201 gcgcgacctg gcggcgcgtg agcggcggcg gatttcccgc cggtttgacg gggcgcatcg
     4261 ggctcgccgc cgccggaggc ggacggatct acgccatcgt ccagtcgaag ctcggcgatc
     4321 tctggcggtc ggacgacggc ggtgcgtcgt ggaagctgat gccgcacaac ccgtacgtcg
     4381 gtgcgcgtcc cttctacttc agccgcatct tcgtcgaccc ggccgatcgc aaccggctga
     4441 tcgacgtcgg gctgattctc tccgcgagca aagacggcgg acgcagcttt catccgattg
     4501 ccgaaaacgc cggctgggat taccacgtcg tgtggtggtc gcacgacggt aggcgcattg
     4561 cggtcggcag cgacgaaggc gcgatctttt ccggtgacgc aggagcgcac ttttggcagc
     4621 cgtacgacct gccgttcgcg caggcgtatc acgccggttt cggcggatcg ctgccaggtt
     4681 acgacgtctg catcggcctg caggacaacg actcgtggtg cgggccatcg gcttcgtata
     4741 acggtatcgg cgtcctcaat cgcgactggt ataccgtcgg ccccggcgac ggaatgtggg
     4801 cgctgatcga cccggcggat caggatctgg tctggagcac gaccaccaac aacgataccg
     4861 gccaagtgta tctgtggaac cgccggacgc agcaagcgta cgaagtttct ccgtccgcgc
     4921 gctacaactc gcaagcgccg gaggcgctgc cgtaccggtt caattgggat acgccgctgg
     4981 cattcgcaag cgttccttcg ccgcacgtat tggtcggcgg caacgtcgtt ttcgagagct
     5041 ccgatcgcgg tcaacattgg acgatcgtca gccccgacct cacgcgcaac gacaagacgc
     5101 accagaaggc ctcgggcgga ccgatctcgc tcgacgtctc gggagccgaa acgtcggata
     5161 cgattctcga cgtcgagacg tcagcgctat cgggcacgct gatctgggtc ggcaccgacg
     5221 acggactcgt acaggtgacc cgcgacggcg gcgctcgctg gagcaacgtc acgccgccgg
     5281 gcgtaccgcc gtgggggcgc gtatcgaccg tcgagccggg acattttgca gccggaaccg
     5341 cgtacgccgc aatcgatcgt cacatgctcg gtgacgagca cccgtacgtc tacgtcaccg
     5401 acgattacgg cgccggctgg cgctctctct ccgcgaatct tccttcgaac ttgttcgtgc
     5461 gctcgattcg cgaggacccc aaggatccgg agctgctcta cgccggcacc cagcgcggcg
     5521 tttggacgtc gttcgatcgc ggccttcact ggcgttcgct gcggctgaac atgccggcga
     5581 cggcgatcta cgacctcgaa atccagccgt ccgccggcga cctgatcgtc gcctcgcacg
     5641 ggcgcggggt ctggatactc gacgaccttc agccgctgcg cgagctggcg cgcgggcgcg
     5701 tctcgggcgt aacgtttttc gcgccccgca gcgcctaccg catgttcgtc gcttcgccga
     5761 tcaattcgtt taccgatgga acgctccccg acaacgagtt cgtcggcgat aacccggaat
     5821 acggcgcgct gctgagttac tacctggacc ggccggcgcg ccgccggccg acgctggaga
     5881 tatccgacgc gcacggccgg atcgtgcgtc atatcgacgg taaagacgtg cccaacgacg
     5941 ccgggatcga tcgcacgagc tgggacctcg ccgaggacgg acccgagcag tggcacggaa
     6001 cctttagcga gaaccgcggc cccgaagagg ggcccgaagt cgttcccggc gcgtacaccg
     6061 cgacgctgaa cgtggacggc cgtgcgtacc ggcagacggt cgacgtgctc gcggatccgc
     6121 gcgacaaaat gccgctcgac gactacgctc gccggcacga ttttctcgag gagatctacg
     6181 ccgaaatcgg cggctgcaac cggatgctca acgggatcga tcgcaagctg aagcaggcga
     6241 acccgcagca ggcgcaggcg ctgcgcgcgt ttcgcgccga gctgaccttc aacccgcgca
     6301 acgtcgaaga cttgggcgag ccgcccgggc tgcgcgatcg cttggtcgac gcgatcgccc
     6361 gctcgagcct ctcctttcaa ccgccgacgg ccgctcagct tagcgaagcc gcggacctga
     6421 aagcgctcta cgcgaacctc tcgcaacggt acgagcaatt gaagtagggt cggcgcgctg
     6481 gcgcccggca gtataatggt ggaaccatga acgtcgacaa gatgaccgag cgcgtcggtg
     6541 aggcgctcaa ctccgcctac tcccgcgcgc tcggcgagca caacacgcag acgacgcccg
     6601 agcacatgct ggcggcgctg ctcgagcagg aacgcggaat cgcccccgac gtgctggcga
     6661 aggccggcgc cgacgtcccg acgctggaac gcgccgccgc cgacgcagtc gctcgcttgc
     6721 cgcggctgac cggcgccaac gccgacaacg cgcaagtcac ggtttcaccc gaactcgcgc
     6781 gtgtgatgac cgcggccgaa aacgaggcca agacgttatc cgacgactac acctcggtcg
     6841 aacacgtgct gttggcgatg gcgcagtcgc ccggagccgt cggccgaatc tttcgcgacg
     6901 ccggcgtgac gcgcgacaaa ctcctgcaag cgttgcgcga ggtgcgcggc aaccagcggg
     6961 tcacgagcaa agaccccgaa ggaacgtaca agtcgctcga acgctacggc cgcgacctca
     7021 cgctcgaagc cgaacgcggc acgctcgatc ccgtgatcgg acgcgacgag gagattcgcc
     7081 ggatcgttca agtgctgtcg cggcgcacca agaacaatcc ggtgctgatc ggcgagccgg
     7141 gcgtcggcaa gaccgcgatc gtcgaagggc tcgcgcagcg gatcgtccgc ggcgacgtgc
     7201 ccgaggggct gaagaacaaa cgcgtcgttt cgctcgacat gggggcgctg atcgccggcg
     7261 ccaaatatcg cggcgagttc gaagagcggc tcaaagccgt gctcaaggac gtcgccaact
     7321 ccgaggggca gatcgttttg ttcgtcgacg aactg
//
LOCUS       DASUCJ010000861         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_376274, whole genome shotgun sequence.
ACCESSION   DASUCJ010000861 DASUCJ010000000
VERSION     DASUCJ010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376274"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFU90_10265"
     CDS             <1..443
                     /locus_tag="VFU90_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480207.1"
                     /translation="FPQLSVAGNLMFALVPTVRGRASREAAIERALREIGLDRCANLH
                     PSALSEDERARVSLMRMLLARPRVLLLDEPYRRFDARTADEMRRFTLAHVRRRGIPTL
                     LATRERAAAEVADGRVIMLAGGRPAAPEAVEEAPRAMRRRVRIA"
     gene            522..1166
                     /gene="msrA"
                     /locus_tag="VFU90_10270"
     CDS             522..1166
                     /gene="msrA"
                     /locus_tag="VFU90_10270"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5480208.1"
                     /translation="MRLITSAMLAVALAGGSAFAAVVPAYTGPAAPVAATRATAVFAG
                     GCFWGVDAVFKHVKGVLDVVSGYSGGSAATAKYLIVGTGATGHAESVQVTYDPSKVTY
                     ADLLRVFFGVAHDPTELDRQGPDYGTQYRSVVFYANDEQKAIALRYIAELDRAKVFPK
                     PIVTQVTPLKGFYPAEAYHQNFLERHPDNPYIVYNDLPKLARLKQDYPDWYRPL"
BASE COUNT          196 a          420 c          439 g          226 t
ORIGIN      
        1 tgtttccgca gctctcggtg gccggcaacc tgatgttcgc gctggtgccg accgtgcgtg
       61 gccgcgcgtc gcgcgaggca gcgatcgagc gcgcgttgcg cgagatcggg ctcgaccgct
      121 gcgcgaatct ccatccgagc gcgttgtcgg aggacgagcg cgcacgcgtc tcgctgatgc
      181 ggatgctgct cgcgcggccg cgcgtgctgc ttctcgacga gccgtatcgg cgcttcgacg
      241 cgcgcactgc cgacgagatg cgtcggttca cgctcgcgca cgtgcgccgt cgcggcattc
      301 ccacgttgct cgcaacccgt gagcgcgccg ccgcggaggt ggccgatgga cgcgtgatca
      361 tgctcgccgg ggggcgtccg gcggcacccg aagcggtgga ggaagcgcca cgcgcgatgc
      421 gccggcgcgt caggatcgcc tgatccaggc atgacggccg cggtgtaacg acggcgcacg
      481 aatcggcgaa ttaccggtat cgaaacgaac aggaggtatc catgagactc atcacttccg
      541 caatgctcgc cgtcgcgctc gccggcggtt ccgcattcgc ggccgtcgtt cccgcttata
      601 cgggtcctgc ggcacccgtt gccgccacgc gggcgaccgc cgtgttcgcc ggagggtgct
      661 tctggggcgt cgatgcggtg ttcaagcacg tcaagggtgt cctcgacgtg gtgtcgggct
      721 attcgggtgg cagcgcggca accgcgaaat acctgatcgt cggaaccggg gcgaccgggc
      781 atgccgaatc ggtgcaggtc acctacgatc cttcgaaggt cacgtatgcg gacctgttgc
      841 gggtcttctt cggcgtcgcc cacgatccga ccgagctcga tcgtcagggc ccggattacg
      901 ggacgcagta ccggtcggtg gtgttctacg cgaacgacga acagaaggcg atcgccctgc
      961 gctacatcgc cgagctcgat cgcgcgaagg tgttcccgaa gccgatcgtc acccaggtga
     1021 cgccgctgaa ggggttctat cccgccgagg cgtaccatca gaacttcctc gagcggcatc
     1081 ccgacaatcc ctacatcgtc tataacgatc taccgaagct cgcccggctg aagcaggact
     1141 atcccgactg gtatcgcccg ctttgaagcg gattgcggct gggcctcccg gcattcccgc
     1201 caccggatgg gtggtgggaa tcgtctgcgc gtgggtgctg ttcggcctcg tcggtcacga
     1261 gccgtggaag cccgacgagg c
//
LOCUS       DASUCJ010000862         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_131529, whole genome shotgun sequence.
ACCESSION   DASUCJ010000862 DASUCJ010000000
VERSION     DASUCJ010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131529"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFU90_10275"
     CDS             complement(<1..230)
                     /locus_tag="VFU90_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480209.1"
                     /translation="MSQDTGGYARHHHAGFHFAGDYGTSAYKSICFDGNTREHDRARS
                     NERRLPNVRSAGQHRARRNVRAVANGAIVRDDR"
     gene            239..>1120
                     /locus_tag="VFU90_10280"
     CDS             239..>1120
                     /locus_tag="VFU90_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5480210.1"
                     /translation="MKPGKTHGRIFLSPPHMGDLEQKYVAEAFASNWIAPLGPHVEAF
                     QREFAATVGVSHALATISGTSALHLALQLVGVGIGDDVFVSTLTFAASVNPVRYLGAT
                     PVLVDSERESWNMDPHLLAQALHERAKIGKLPKAVVVVHLYGQSADMDPILESCRKYE
                     VPLIEDAAEALGATYKGRPVGTFGRAGVFSFNGNKIITTSGGGMLVSEDRRLVTHAFK
                     LATQARDPAPYYEHSQVGYNYRMSNVLAGIGRGQLRMLSDRVAARRRNFEFYTAALGD
                     VPGIAFMPEAPWGTHTRW"
BASE COUNT          192 a          371 c          366 g          191 t
ORIGIN      
        1 cggtcgtcgc gcacgattgc accgttggcg acggcgcgca cattgcgccg ggcgcggtgc
       61 tggccggcgg agcgcacgtt gggcagtcga cgctcgttgg agcgcgcgcg gtcgtgctcc
      121 cgggtgttac catcgaagca gatgctcttg taggcgctgg ttccgtagtc gccggcgaag
      181 tgaaagccgg catggtggtg gcgggcgtac ccgcccgtgt cttgagacaa cgagcgacat
      241 gaaacccggg aaaacgcacg gacgcatttt cctgtcgccc ccgcacatgg gcgacctcga
      301 gcagaaatac gttgctgaag cgttcgcatc caactggatc gcgccgctcg gtccacacgt
      361 cgaagcgttt cagcgcgaat tcgcggcgac cgtcggcgtg tcgcacgcac tcgcgaccat
      421 ctcgggcacc tcggcgctgc acctcgcgct gcagctcgtc ggcgtcggca ttggcgacga
      481 cgtcttcgtt agtacgctga cgttcgccgc gagcgtcaat cccgttcgct acctgggcgc
      541 cacgccggtg ctcgtcgaca gcgagcgtga atcgtggaac atggatccgc accttctcgc
      601 gcaggcgctg cacgaacgcg cgaagatcgg caagctgccg aaagcggtcg tcgtcgtgca
      661 cctctatggg cagagcgccg acatggatcc gatcctcgag agctgccgca aatacgaagt
      721 gccgctcatc gaggatgcag ccgaagcgct cggcgccacg tacaagggac gtcccgtcgg
      781 cacgttcgga cgcgcgggcg tgttctcgtt caacggaaac aagatcatca ccacgtccgg
      841 cggcggcatg ctcgtgagcg aagaccgtcg tttggtcacg cacgcgttca agctcgcgac
      901 acaagcgcga gatcccgcgc cgtactacga gcactcgcag gtgggctaca attatcgcat
      961 gagcaacgtg ctcgccggca tcggtcgcgg gcagttgcgc atgttgagcg atcgcgtcgc
     1021 cgcgcgccgc cgcaacttcg agttctacac cgccgctttg ggcgacgtcc ccggcatcgc
     1081 gttcatgccc gaagcgccct ggggcaccca cacgcgctgg
//
LOCUS       DASUCJ010000863         1362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1155144, whole genome shotgun sequence.
ACCESSION   DASUCJ010000863 DASUCJ010000000
VERSION     DASUCJ010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155144"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..742
                     /locus_tag="VFU90_10285"
     CDS             158..742
                     /locus_tag="VFU90_10285"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU5480211.1"
                     /translation="MSVLPQPPGRLLTIREYADLGEIEHGRYELQEGRLVMSPSPTPD
                     HMIAIARLYVALERQIPNDFAAVPDVDVDLQLAPATHPGSSRRPDLLIVRREALGRVR
                     KERGLLRAGEVLIVIEVVSPGSERTDYVVKRGEYAGAGIPYYWVIDLDPSGSLTAYQL
                     AGGFGYQENATFTGTFETDTPFAVRLKLEELRHK"
     gene            complement(745..924)
                     /locus_tag="VFU90_10290"
     CDS             complement(745..924)
                     /locus_tag="VFU90_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480212.1"
                     /translation="MLKAAPPGITSSNSNVLTARGALIRGVAAGVADVRIHQSTLCGS
                     AIVVACTLMRVTVVP"
BASE COUNT          246 a          474 c          431 g          211 t
ORIGIN      
        1 ccgaggctga gcgcaacctg tgaggcgagc caccggccgc gagggccagt gcggcagcca
       61 ccccgtgccc aagcgccgcg gcgcttcgcc ctgtggataa ccgcgttcac tgaacgcgca
      121 ccgtgacacg atggacgccg acgtgaggag aaccgcgatg agcgtccttc cccagccgcc
      181 gggccggttg ctgaccatcc gcgagtacgc ggatctcggc gagatcgagc acggccgata
      241 cgagctgcag gaggggcgcc tggtgatgtc accgagcccg acgcccgatc acatgatcgc
      301 gatcgctcgg ctgtatgtcg cgctcgagcg gcaaatcccg aacgactttg cggcggtgcc
      361 ggacgtcgac gtcgacctgc aactcgcgcc cgccactcat cccggctcgt cgcgacggcc
      421 agatctgctc atcgtccggc gtgaggcgtt ggggcgggtg cggaaggagc gcggcctact
      481 ccgagccggc gaggtgctca tcgtgatcga ggtcgtctcg ccgggatccg agcgcaccga
      541 ctacgtggtc aagcgtggtg aatacgccgg cgccggcatc ccctattact gggtcatcga
      601 tctggatccc agcgggtcgc tgaccgcgta tcaactggcg ggcggcttcg gctatcaaga
      661 gaacgcaacg ttcaccggca cattcgagac cgacacgccc ttcgcggtgc gactcaagct
      721 cgaagaactg cgccacaaat agcctcacgg cacaacggtg actcgcatca gcgtgcatgc
      781 gaccacaatc gcgctcccgc acaacgtcga ctggtgaatc cggacgtcgg ccacgcccgc
      841 ggcaacgccg cgtatcagcg cgccacgcgc cgtcagaaca ttggagttgg acgacgtgat
      901 gccgggcgga gcggctttaa gcatgcgcac ggtcgcggtc tctccgactc gaatcttgac
      961 cggcgtcccg acagcgacgc cggcgcagtt caacaaccct tcgcgggtcc cgttgctatc
     1021 gaggtagaca acgtcaacgc cgcagttcgt cttcacgccg ctggttgttg cgcaactcgc
     1081 cgtcacagcc gccgtgctca gcagcaggcc ggaggcgatc gctgcccgac caaatcgccc
     1141 gactttgtta accccaacca tcgtcttcac acgcgtcaga cgcagccgcg aaggcgctcg
     1201 ttccatcgcg cagcgaggcg cgccattccg ccttcaagca ctgcgcccgt cgagtcgtcg
     1261 ttcacggccg caggtaatcg ctggccggac agatcaccgc tctgaccgag cgcacggggt
     1321 cagccggtca gaaggatcgg cgtgcgctaa ccgagacggc cg
//
LOCUS       DASUCJ010000864         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_9277, whole genome shotgun sequence.
ACCESSION   DASUCJ010000864 DASUCJ010000000
VERSION     DASUCJ010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9277"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1012)
                     /locus_tag="VFU90_10295"
     CDS             complement(170..1012)
                     /locus_tag="VFU90_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5480213.1"
                     /translation="MHSGPLPLEDTFGDVLRKAMRGNGVDKAQLARATGIDERDVAAW
                     LKDEGAAAEAQARAIATVLHLDPGKLADTAAGRWRPPPIERADVRHHPQNPHPSNGYV
                     FFLDGGRRAALVDPAGIPQNLLRVLRDGDYHLQYVLITHKHADHCDATADVAKAFPQA
                     RIVMHDLDVHAIGPLASKALRVRDGEELPFGDGARIRMLHTPGHTDGSSCYLFDAALF
                     SGDTLFAASVGGAFGDASTYADILNSVRSKLFTLPGDTVVMPGHGPPTTIALERAHNP
                     FFPG"
     gene            1114..>1743
                     /locus_tag="VFU90_10300"
     CDS             1114..>1743
                     /locus_tag="VFU90_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480214.1"
                     /translation="MERVIVIGSGPAGLTAAIYAARANLEPLVLAGGLYGGQLMLTTE
                     VENYPGFPEGIMGPDLMIRFREQAERFGARIENVDATSVDFGRQPFIVRTAEHEYHGK
                     TVIVATGASARWLGIPGETQLRGRGVSTCATCDGAFFRDRNIVVVGGGDSAMEEALFL
                     TRFGRRVTVIHRRDHLRASKIMAERARSHEKIDFIWNTVVEEVLGETHMK"
BASE COUNT          290 a          549 c          603 g          301 t
ORIGIN      
        1 acagcaaggg tcgccgtcct tcgactgcgg cgctgcgcgc ctccgctcag gatgacatag
       61 ctagggtcgc cgtccttcga ctgcggcgct gcgcgcctcc gctcaggatg acacagctac
      121 ggtcctccgc tcaggatgac acagctgagg cgcttcgctg aggaggacgt tagcccggaa
      181 agaatgggtt gtgcgcgcgc tccagcgcga tcgtggtcgg cggtccgtgg ccgggcatga
      241 cgaccgtgtc gccgggaagc gtgaagagct tgctgcgtac gctgttgaga atgtcggcat
      301 aggtgctcgc atcgccgaat gcgccgccga cgctggccgc aaagagcgtg tcgccgctga
      361 acagggccgc atcgaaaaga tagcacgagg atccgtcggt atggcccggc gtgtgcagca
      421 ttcggatccg cgcgccgtca ccaaacggca gctcttcgcc gtcgcgcacg cgcagagcct
      481 tggacgccaa cgggccgatt gcgtggacgt cgaggtcgtg catgacgatt cgggcctgcg
      541 gaaaggcctt cgcgacgtcg gcggtggcgt cgcaatgatc ggcatgcttg tgcgtgatga
      601 ggacgtactg gagatggtag tccccgtcgc gcagcacccg cagcaggttt tgcggaattc
      661 ccgccggatc gaccaaggcc gcccggcggc cgccgtcgag aaagaagacg tatccgttgc
      721 tgggatgcgg attttgcgga tggtgccgca cgtcggcgcg ttcgatcggc ggcggacgcc
      781 accgcccggc cgcggtgtcg gcgagtttgc ccgggtcgag gtgcagcacg gtcgcgatcg
      841 cgcgggcttg cgcctcggcg gcggcacctt cgtccttcag ccaggcggca acgtcgcgtt
      901 cgtcgattcc ggtcgcgcgc gcgagctgcg ctttgtcgac gccattgccg cgcatcgctt
      961 tgcgcaggac gtcgccgaac gtatcttcca ggggcaacgg tccggagtgc atgcgcgtgc
     1021 gttcgacgtg cctcatccca agtcacctcg ccgggcggct cgcgttcgta gtccgcaacc
     1081 ggaaaggcag cgcgggcggt cgaaccggtc ggaatggaac gcgtgatcgt catcggctcc
     1141 ggcccggcgg ggcttacggc cgccatctac gcggcacgag cgaatctcga gccgctggtg
     1201 ctggcgggcg gtctgtacgg cggtcagctg atgctgacga ccgaggtgga gaactacccc
     1261 ggctttcccg aaggcatcat ggggcccgat ttgatgatcc gttttcgcga acaagccgaa
     1321 cgcttcggcg cgcgaatcga aaacgtcgac gccacttcgg tcgacttcgg gcggcagccg
     1381 tttatcgtcc ggacggccga gcacgaatac cacggcaaaa cggtgatcgt cgcaaccggc
     1441 gccagcgcgc gctggctcgg catccccgga gaaacgcagc tgcgcggtcg cggcgtttct
     1501 acctgtgcta cctgcgacgg agcgttcttc cgcgaccgga acatcgtcgt ggtcggcggc
     1561 ggcgactccg cgatggaaga ggcgcttttt ctgacgcgct ttggacgccg ggtcaccgtc
     1621 attcaccgtc gcgaccatct gcgcgcgagc aaaatcatgg ccgagcgcgc ccgcagccac
     1681 gaaaagatcg acttcatttg gaacacggta gtcgaggaag tcttagggga aacgcacatg
     1741 aag
//
LOCUS       DASUCJ010000865         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_559441, whole genome shotgun sequence.
ACCESSION   DASUCJ010000865 DASUCJ010000000
VERSION     DASUCJ010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559441"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VFU90_10305"
     CDS             complement(<1..1663)
                     /locus_tag="VFU90_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480215.1"
                     /translation="MYRPDKPEVKRRVDWRRMGALFAPYWGQQLTVLACIVVASVLGL
                     VPGFVTARLIDVAIPRRDLRELSIDVGLILASALTAAAVGVLQGYLNSLVGEGIMRDI
                     RTQLVAHLHRMPLTFFTGTKTGEIMNRVSNDVDNIDNVVTGTLTSIVTNVVVIASTLI
                     AMFVWNWRLALLSIVIVPLMILPLGPVGRRMYDIRKKTREKRDEIESITQETLSISGI
                     TLIKSFAREAYERSRFYDVGTRLMRLEIDLAMVGRWFLASVTAMIVIGPALVWFGGGW
                     YAIAGGLQVGVIVAFVSFIQGRLYGPAAALAGIQVQIVSALAVFERIFDYLDMQAEEY
                     VPAGAVALPGVRGDIAFDNVAFSYDESRRILDGVSFRVAAGQAVAFVGPSGGGKTTIT
                     SLVPRFYDPQEGRVSVDGHDVRTLELESLRRNIGIVTQETYLFHDTIANNLRYGNPDA
                     TDADLEAAARAASIHDFIATLPEGYATVVGERGHKLSGGERQRLAIARVLLKDPRILI
                     LDEATSSLDSENEAAIQGALLTAMRGRTSLVIAHRLSTILAADTIF"
     gene            complement(1724..>2033)
                     /locus_tag="VFU90_10310"
     CDS             complement(1724..>2033)
                     /locus_tag="VFU90_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480216.1"
                     /translation="WYENALVLTFAGPYVEDGVEKYNQANVGGGYFSLMAHEWMEREP
                     GSASGVVFEVDDIDATVAGLRAKGVAASDVYATPVCKISSFDDPEGNKVSLHQITVPH
                     "
BASE COUNT          365 a          656 c          662 g          350 t
ORIGIN      
        1 cgaagatagt atcggccgcg aggatcgtcg aaagacggtg cgcgatcacc aggctcgtgc
       61 ggccgcgcat cgccgtcagc agcgcgcctt ggattgccgc ctcgttctcg gaatccagcg
      121 agctagtcgc ttcgtcgaga atcaggatgc gcggatcttt gagcaaaacg cgggcgatcg
      181 cgagtcgctg gcgttcgccg ccggacagtt tgtggccgcg ctcgccgacg accgttgcgt
      241 acccttcggg cagcgtcgcg atgaaatcgt gaatgctggc cgcgcgcgcg gccgcctcga
      301 gatcggcgtc ggtggcatcg ggattgccgt accggagatt gttggcgatc gtgtcgtgga
      361 agaggtaggt ctcctgggtg acgatcccga tgttgcgccg caacgattcc agctcgagcg
      421 ttcgcacgtc gtgtccgtcg accgagacgc ggccttcctg cgggtcgtag aagcgcggca
      481 cgagcgacgt gatcgtcgtc ttccctccac ccgagggccc gacgaacgcg accgcctggc
      541 cggcggcgac gcggaacgag acgccgtcga gaatgcggcg cgactcgtcg tacgaaaacg
      601 ccacgttgtc gaaagcgatg tcgccgcgaa ctcccggcag cgcgaccgct cccgccggca
      661 cgtactcttc ggcttgcatg tcgagataat cgaagatgcg ctcgaagacc gcgagcgcgc
      721 tgacgatctg aacctgaatt cccgccagcg ccgcggccgg cccgtagagg cggccttgga
      781 tgaacgaaac gaaggcgacg atgacgccca cttgcagccc gcccgcgatt gcgtaccagc
      841 cgccgccgaa ccacaccagc gccggcccga tcacgatcat cgcggtgacc gacgccagaa
      901 accagcggcc gaccatcgcg agatcgattt cgaggcgcat cagccgggtt ccgacgtcgt
      961 agaagcgcga ccgttcgtac gcttcgcgcg cgaacgactt gatgagcgta atccccgaaa
     1021 tcgaaagcgt ctcttgggta atcgactcga tctcgtcgcg tttctcgcgg gtcttcttgc
     1081 gtatgtcgta catgcggcgg ccgaccggcc ccagcggcag gatcatcaac gggacgatca
     1141 cgatcgagag cagcgcgagg cgccagttcc agacgaacat cgcgatcagc gtcgaggcga
     1201 tcacgaccac gttggtgacg atcgaggtga gcgttccggt tacgacgttg tcgatattgt
     1261 cgacgtcgtt gctgacgcgg ttcatgatct cgccggtttt cgttccggtg aagaacgtca
     1321 gcggcatgcg gtgcaggtgc gccaccagct gcgtgcggat gtcgcgcatg atgccttcgc
     1381 ccacgagcga gttcagataa ccctgcaaga cgccgacggc ggccgccgtc agcgccgagg
     1441 cgaggatgag gcctacgtcg atgctcagct ccctgaggtc gcgccgcggg atcgcgacgt
     1501 cgatgagacg ggcggtcacg aagccgggca cgaggccgag caccgacgcg acgacgatac
     1561 acgccagcac ggtgagctgc tgtccccagt acggtgcgaa cagcgcgccc atccgccgcc
     1621 aatcgacccg ccgtttcacc tcgggtttgt cgggacggta catggccgac cacattaggc
     1681 cgtggccgct catcatggcg cggaaacctg cttccggcgg gcgctagtgc ggaacggtga
     1741 tctgatggag cgagaccttg ttgccttcgg gatcgtcgaa cgacgagatc ttacaaaccg
     1801 gcgtcgcgta cacgtcggaa gcggcgacgc ccttggcgcg cagaccggcc accgtcgcgt
     1861 cgatgtcgtc gacttcgaag acgacgccgg acgcgctgcc cggttcgcgc tccatccact
     1921 cgtgcgccat caggctgaag tagccgccgc cgacgttggc ttgattgtat ttttcgactc
     1981 cgtcttccac gtacggcccg gcgaacgtca gtacgagcgc gttctcgtac cat
//
LOCUS       DASUCJ010000866         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1002604, whole genome shotgun sequence.
ACCESSION   DASUCJ010000866 DASUCJ010000000
VERSION     DASUCJ010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002604"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="glmS"
                     /locus_tag="VFU90_10315"
     CDS             <1..1105
                     /gene="glmS"
                     /locus_tag="VFU90_10315"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5480217.1"
                     /translation="MIERGGFPHFMLKGIFEQPTTIENTMRGRLLVEEGTAKLGGINL
                     TNEELLEFDNIVITACGTSWHSALIGEFMIEELARIPVEVEYASEFRYRNPVIGPRTL
                     CIVISQSGETADTLAAMREAQKRGARALGIVNVVGSTIARESDGGIYIHAGPEIGVAS
                     TKAFTSQVIALALFTLRLARLRGLPAEEGRPIVEALQKLPAQIKDVLDRADEIESIAD
                     EFNDAANFLYLGRGYNFPAALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVV
                     FIAPHDSVFDKVVSNVQEVKARGGRVIALTSRSEPSLAGKIDYEIRVPETIDMFTPIL
                     AAVPLQLLAYYIAVKRGANVDQPRNLAKSVTVE"
     gene            1165..>2135
                     /locus_tag="VFU90_10320"
     CDS             1165..>2135
                     /locus_tag="VFU90_10320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5480218.1"
                     /translation="MHYVVTGGAGFIGSHLVEHLVTAAGGHRVTVLDNFSTGKRENLA
                     PWLDRVRVVEGSVTDPNACREAIAGADFVLHQAALPSVPRSLRDPVASHEANATGTLN
                     VLIAARDEHVKRVVYAASSSAYGNTAELPKREEMLPRPLSPYAAAKYAGEQYCRAFHA
                     SFGVGTIALRYFNVFGPRQDPTSQYAAVIPKFIVCAQAGESPTIFGDGEQTRDFTFVR
                     NVVRANLLACEAPPAALGEAYNVGCGERVSVNALWRRIRDLLGATVEARYEPSRTGDV
                     RDSLASLDRVHAMLGYAPVVALDDGLRETIRSLTGREPAAVGSPAAVR"
BASE COUNT          372 a          690 c          720 g          353 t
ORIGIN      
        1 catgatcgag cgaggtggat tcccgcactt catgctcaag gggatcttcg agcagccgac
       61 caccatcgag aacacgatgc gcggccgcct gctggtggaa gagggcaccg ccaaactggg
      121 cggcatcaac ctgaccaacg aagaactgct cgagttcgac aacatcgtca ttaccgcctg
      181 cggcaccagt tggcactcgg cgctcatcgg cgagttcatg atcgaggagc tggcgcgcat
      241 tccggtggaa gtcgagtacg cgtccgagtt ccggtaccgg aatccggtga tcggtcccag
      301 gacgctgtgc atcgtgatct cgcagtccgg cgaaacggcc gacacgctgg ccgcgatgcg
      361 cgaggcccag aagcgcggcg cacgagcgtt aggcatcgtg aacgtggtcg gctcgacgat
      421 cgcccgcgag tcggacggtg gtatctacat tcacgccggt ccggaaatcg gcgtcgcgtc
      481 caccaaggca ttcacgagcc aggtgatagc gttggcgctg ttcacgctgc ggctggcgcg
      541 cctgcgtgga ctgccggcag aggaaggccg cccgatcgtc gaagcgctgc agaagctccc
      601 ggcccagatc aaggacgtgc tggatcgcgc cgacgagatc gaaagtatcg ccgacgagtt
      661 caacgacgcc gcgaattttc tctatctggg ccgcgggtac aatttccccg ccgcgctcga
      721 gggcgcgctc aagctcaagg agatcagcta catccacgcc gaagggtatc cagcggcgga
      781 gatgaagcac ggtcccatcg cgctcatcga cgaaaacatg ccggtggtgt tcatcgcgcc
      841 gcatgactcg gtgttcgaca aggtcgtgtc gaacgtgcag gaagtgaagg ctcgcggcgg
      901 ccgggtgatt gcgctcacga gccggtcgga gccgtcgctg gccggcaaga tcgactacga
      961 gattcgcgtc cctgaaacga tcgacatgtt cacgccgatc ctggccgcgg ttccgctgca
     1021 gttgctcgcg tactacatcg ccgtgaagcg gggggcgaac gtggaccagc cgcgcaacct
     1081 cgccaagtcg gtgacggtcg agtagcctaa cgcatctggg cgccggatcc cggcgcccac
     1141 ccctgcaccc ggacgcgttt caccgtgcac tacgtcgtga caggaggggc gggcttcatc
     1201 ggctcgcacc tcgtggaaca tctcgtgacc gccgccggcg gtcatcgcgt cacggtgctc
     1261 gacaacttct cgacgggcaa acgcgagaac ctcgcgccat ggctcgatcg ggtccgcgtt
     1321 gtcgagggat cggtcaccga tccgaatgct tgccgggaag caatcgccgg agcggatttc
     1381 gtgctgcacc aggcggcgct tccgtcggtt ccgcggtcgc tgcgcgatcc cgtggcctcg
     1441 cacgaagcca atgcgaccgg aacgctcaac gtgctcattg cggcgcgcga cgagcacgtg
     1501 aagcgcgtgg tgtacgcggc gtcatcctcg gcctatggta acacagccga gctccccaag
     1561 cgcgaggaga tgctgccgcg gccgttgtcg ccgtacgcgg cggcgaagta cgccggcgag
     1621 cagtattgcc gcgcgtttca cgcgtcgttc ggcgtgggaa cgatcgcgct gcggtacttc
     1681 aacgtgtttg gaccgcggca ggatcccacc tcgcagtacg ccgcggtgat cccgaagttc
     1741 atcgtgtgcg cgcaggcggg cgagtcgccc acgatcttcg gcgacggcga gcagacacgg
     1801 gacttcacgt tcgtccgaaa cgtcgtccgc gccaacctgc tggcgtgcga ggctccgccg
     1861 gccgcgttag gcgaagcgta caacgtcggc tgcggcgagc gcgtgagcgt caacgcgctt
     1921 tggcgccgca ttcgcgacct gctcggcgcg acggtcgagg cgcggtacga gccctcgcgc
     1981 acgggcgacg tccgcgattc gctcgcctcg ctcgatcggg tgcatgccat gctcggctat
     2041 gcaccggtgg tggcattgga cgacggattg cgagagacca tccggtcgct caccggacgg
     2101 gaaccggccg cggtgggatc gcccgcggcc gtgcg
//
LOCUS       DASUCJ010000867         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_161960, whole genome shotgun sequence.
ACCESSION   DASUCJ010000867 DASUCJ010000000
VERSION     DASUCJ010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161960"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..772
                     /locus_tag="VFU90_10325"
     CDS             149..772
                     /locus_tag="VFU90_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5480219.1"
                     /translation="MTARAAAAVIAAIAFGAFLALGFAVKPGEPPALAGWEIALQNHS
                     DLIAWWLTWACYAQLLLPLAIVLLIVAWRVPAWRARIIFSVVMLVLCWQGADVFQHFF
                     ARPRRLDWVVKHETAFSYPSSHAAIATGFYALWAAMLYASDLPRAVRTVVPVLLVLLA
                     LGVCWSRLALGAHYLTDLFGGALLAAALVAAALAVVPVKVLARPARV"
     gene            779..1921
                     /locus_tag="VFU90_10330"
     CDS             779..1921
                     /locus_tag="VFU90_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEU5480220.1"
                     /translation="MTYPDPVLAAIERKVESDIPLSFEDGLALYRTHDLHALGRMARK
                     RKERKSGNRVFYVLNRYINSTNVCFAACTFCSFAADEFKEPQRVFRMTADQVFAKATE
                     TGTNFNQIHIVGGHDPRQLSLDYWLPLMRRFKQALPHVQLSLFTAAEIEYMAKRHRLG
                     FPEIVAALKEAGLDNVNGGAAEIFAEATRAKICPNKVSSANWLAIHEELHRQGVASNA
                     SMLYGHIESLDDRVDHLIRLRESQDRSPGFNTCIPLAFHPDGNELSYCGWTTGLDDLK
                     TFAVARLMLHNFDHIKAYWMIQGLKVCQVALQFGADDMDGTHGSTDEEMIYHSAGTRS
                     GQYVDDREFRRLIEDAGYVPVRRNSTYDEFAWDWTPPPAAAELVGV"
     gene            1918..2754
                     /locus_tag="VFU90_10335"
     CDS             1918..2754
                     /locus_tag="VFU90_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEU5480221.1"
                     /translation="MSLRCGRIRYTNALPVYAAFDAGASVHPGSLHADVPARLNAMLL
                     AGELDLSPISAFAWARDCEQLVLLPDLCIGARDDVVSVILVSATPPALLGGKTVYVTP
                     ESESGHNLLRVLLERRYAVAPVYAVDAQAPARARAGEPTLLIGDAAIDALEAVPPAHV
                     YDLGKLWHDWTGRQTVFAVWAARRDVYARDPEGVRACMEALTDSYTWSRSHGDAVLAL
                     AQRAHPRPPGFYETYYGKLNFAFHTAAQRGLSAFCRELYELGAIATVPPATPEVLGAV
                     AR"
     gene            2738..3823
                     /gene="mqnC"
                     /locus_tag="VFU90_10340"
     CDS             2738..3823
                     /gene="mqnC"
                     /locus_tag="VFU90_10340"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEU5480222.1"
                     /translation="MPSLASLLDRAATGGRLTLDEGVRLYQEADLHALGAAAHARRMQ
                     LHPTDVVTYVIDTTINYTNVCNVHCTFCAFFRPEKHPEGYTMSHDTVLERVKFAADQG
                     ATQIMIQGGVNPELGIGWFETLFDRVRREYPNVDIHSLSVSEIVGLAHVERSSVRDVL
                     ARLKDAGMKSLPGAGAEILVERVRKRISARKVKPDEWLGVMREAQLLGMPTTATMMFG
                     SIETAAERIEHMHVLRELQDETGGFTAFIPWYYVPYKTPLRGKEATGLEYLRVLALSR
                     LYLDNFAHLQASWLTPGLKLGQLALFYGCDDMGGTILEERVVHDAGSTNEATRRDLEN
                     LIRSAGFVPVVRDTYWNVRDDVALATA"
     gene            complement(3836..>4534)
                     /locus_tag="VFU90_10345"
     CDS             complement(3836..>4534)
                     /locus_tag="VFU90_10345"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEU5480223.1"
                     /translation="YARIERRDDGVHLFRSRHAKDQAYALAQLGADRLERLLLPLGAL
                     DKPATREHARRLGLPVHDKTESQDICFVEGGDYRDVVERIRPGLRRPGEIVATSGERI
                     GSHDGIARYTVGQRAGLPPASDGARYVTRIDSDTNTIVVGREDELLSSRLQAGSLNLI
                     RPERFERGEIRVLAMVRYRAKPAPAIARANADETLELQFDDPQRAVTPGQLVALLDPG
                     GDEVLGAATIARAA"
BASE COUNT          730 a         1507 c         1525 g          772 t
ORIGIN      
        1 tgaaacagct cgaggagctg aaagtcgtcg gtccgcacga aggcaccaaa ccgcgcaaga
       61 ttctggtcgg accgtcggag ctcgaaatga tggcgccgcg cttgggcaag ggtgagggag
      121 ggcagcagga cctgttcgcc agcggctctt gacggcgcga gccgccgcgg ccgtcatcgc
      181 cgcgatcgcg ttcggtgcgt tcctggcgct cgggtttgcg gtcaagcccg gcgagccgcc
      241 cgcgttagcc ggttgggaaa tcgcgctgca gaaccacagt gacttgatcg cctggtggct
      301 gacgtgggct tgctacgcgc agctgctgct gccgctcgcg atcgtgctgc tgatcgtcgc
      361 ctggcgcgtg ccggcttggc gcgcgcggat aattttcagc gtcgtaatgc tcgtgctgtg
      421 ctggcaaggc gccgacgtct tccagcattt cttcgcacgg ccgcgccggc tcgactgggt
      481 ggtcaagcac gagacggcct tttcgtaccc cagctcgcac gctgcgatcg cgaccggctt
      541 ctacgcgctt tgggccgcga tgctctacgc ttcagacttg ccgcgcgccg tgcggaccgt
      601 cgtccccgta ctgctggtgc tgctggcgct gggcgtctgc tggtcgcggc tcgcgctggg
      661 cgcccactac ctgaccgatc tgttcggcgg cgccctgctg gcggcggcgc tcgtcgccgc
      721 ggcgctggcg gtggttccgg taaaggtctt agcgcggcct gctagagtat aactgaaggt
      781 gacgtacccg gatcccgttc tcgcagcgat cgaacgcaaa gtcgaatccg acatcccgct
      841 ttcgtttgaa gacgggctcg cgctctaccg gacgcacgat ctgcacgctc tcggccgaat
      901 ggcccgcaag cgcaaggagc gcaagagcgg gaaccgcgtt ttctacgtac tgaaccgcta
      961 catcaactcg acaaacgtct gcttcgccgc ctgcacgttc tgctcgtttg ccgccgacga
     1021 gttcaaggag ccgcagcggg tcttccggat gacggccgat caggtgttcg ccaaggcgac
     1081 cgagaccggc acgaatttca accaaatcca catcgtcggc gggcacgatc cgcggcagct
     1141 gtcgctcgat tactggcttc cgctgatgcg ccgcttcaag caggcgctcc cgcacgtgca
     1201 gctgtcgctc ttcacggcgg ccgaaatcga atacatggcc aagcgccatc gtcttgggtt
     1261 tcccgagatc gtcgccgcgc tcaaggaggc cgggctcgac aacgtcaacg gcggtgcggc
     1321 cgagatcttt gcggaagcga cgcgtgcgaa aatttgcccg aacaaggtct cgagcgccaa
     1381 ctggctggcg atccacgaag aactgcatcg ccaaggcgtg gccagcaacg ccagcatgct
     1441 gtacggccac atcgagtcgc tcgacgatcg cgtcgaccat ctgatccggc tgcgcgaatc
     1501 gcaggaccgc tcgcccggct tcaacacgtg cattccgcta gcgttccatc ccgacggtaa
     1561 cgagctgtcg tactgcgggt ggacgaccgg cctcgacgac ttgaaaacct tcgcggtagc
     1621 gcggctcatg ctgcacaact tcgatcacat caaagcctac tggatgattc aaggcctgaa
     1681 ggtctgtcaa gtcgcgctgc agttcggcgc cgacgacatg gacggcacgc acggatcgac
     1741 cgacgaagag atgatttacc actcggccgg aacgcgttcc ggacagtacg tggacgaccg
     1801 cgagttccgg cggctgatcg aggacgccgg ctacgtgccg gtgcgccgca actcgactta
     1861 cgacgagttc gcctgggatt ggacgccgcc gccggcggca gccgagctag tcggcgtatg
     1921 agcctgcgct gcgggcgcat ccgctacacc aacgcgctgc cggtgtacgc cgctttcgac
     1981 gcgggcgcga gcgtccaccc gggttcgctg cacgccgacg tgcccgcgcg gctgaacgcg
     2041 atgctgctcg cgggcgaact cgatttgagc ccgatcagcg cgttcgcctg ggcgcgcgat
     2101 tgcgagcagc tcgtgctgct gccggatctc tgcatcggcg cgcgcgacga cgtcgtctcg
     2161 gtgatcctgg tgtcggcgac gccgcccgcg ctgctcggcg ggaagacggt ctacgtgacg
     2221 cccgagtcgg aaagcgggca caacctgctg cgcgttctgc tcgagcggcg ctacgccgtc
     2281 gcgccggtct acgcggtcga cgcgcaggcg ccggcgcgcg ctcgcgccgg cgaacccacg
     2341 ctgctgatcg gcgacgccgc gatcgacgcg ctcgaagcgg ttccaccggc gcacgtatac
     2401 gatctgggaa agctctggca cgactggacc ggccggcaga cggtatttgc ggtgtgggcg
     2461 gcgcgccgcg acgtctacgc gcgcgatccc gaaggcgtgc gcgcctgcat ggaagcgctc
     2521 accgattcct atacgtggtc gcgttcgcac ggcgacgccg tgctcgcgct cgcgcagcgc
     2581 gcgcatccgc ggccgcccgg attctacgaa acgtactacg gcaagctgaa tttcgcgttt
     2641 cacactgccg cgcagcgcgg actttcggcg ttttgccgcg agctgtacga gctcggcgcg
     2701 atcgcgacgg ttccgccggc gactccggag gtgctcggtg ccgtcgctcg ctagtttgct
     2761 cgaccgggcc gcgaccggcg gccggctgac gctcgacgaa ggcgtgcgcc tctaccaaga
     2821 agccgacctg cacgcgctcg gggccgcggc ccacgcgcgg cggatgcagc tgcaccccac
     2881 cgacgtcgtg acctacgtga tcgacacgac gatcaactac accaacgtgt gtaacgtgca
     2941 ctgcaccttc tgtgcgttct tccggccgga gaagcacccc gaaggctaca cgatgtcgca
     3001 cgacaccgtg ctcgagcggg tgaagtttgc ggccgatcaa ggcgccacgc agatcatgat
     3061 tcaaggcggc gtcaatccgg agctcggcat cggctggttc gagacgctgt tcgatcgcgt
     3121 gcgccgcgag tatccgaacg tcgacatcca ctcgctgtcg gtttcggaga tcgtcgggtt
     3181 ggcgcacgtc gagcgctcga gcgtgcgcga cgtgctcgcg cgcctcaagg atgcgggcat
     3241 gaagtcgctt cccggcgccg gcgcggagat cttggtggag cgcgttcgca aacgcatctc
     3301 ggcgcgcaag gtaaagcccg acgaatggct cggcgtgatg cgcgaggccc agctgctcgg
     3361 aatgccgacg accgccacga tgatgttcgg ttcgatcgaa accgcggcgg agcgcatcga
     3421 gcacatgcac gtgctgcgcg agctgcaaga cgaaaccggc ggatttacgg cgttcatccc
     3481 gtggtactac gtgccctaca aaacgccgct gcgcggaaag gaagccaccg gcctcgagta
     3541 cctgcgcgtg ctggcgcttt cgcggctcta tctggacaac ttcgcccacc tgcaagccag
     3601 ttggctaacg ccggggctga agctggggca gctggcgtta ttctacggct gcgacgacat
     3661 gggcggcacg attctcgagg agcgcgtcgt ccacgacgcc gggagcacca acgaagcgac
     3721 ccgccgcgac ctggaaaatc tcattcgcag cgcgggtttc gtgccggtcg ttcgcgatac
     3781 gtattggaac gtacgcgacg acgtggcgct cgcgaccgcc tagggtaagc cgggcttacg
     3841 cggcccgcgc gatcgtcgcc gcgcccagga cctcgtcgcc gccgggatcg agcagcgcga
     3901 ccagctggcc cggcgtcacc gcccgctgcg gatcgtcgaa ctgcagctcc aacgtttcgt
     3961 cggcgttggc gcgggcgatc gccggcgcgg gcttggcgcg gtagcgtacc atcgcgagca
     4021 cgcggatttc gccgcgctcg aagcgctcgg ggcgaatgag gttgagcgat cccgcctgca
     4081 ggcgcgagga aagcagttcg tcttcgcggc cgacgacgat cgtgttggtg tccgaatcga
     4141 tgcgcgtcac gtagcgggcg ccgtccgacg ccggcggcaa gccggcgcgc tggccgacgg
     4201 tgtagcgcgc gatgccgtcg tgcgagccga tccgctcgcc gctggtcgcg acgatctcgc
     4261 ccggacggcg caagccggga cgaatgcgct cgaccacgtc gcggtagtcg ccgccttcca
     4321 cgaagcagat gtcttgcgat tcggtcttgt cgtgaaccgg caaacccagc cggcgcgcgt
     4381 gttcgcgcgt cgcgggcttg tcgagcgcgc cgagcggcag cagcaaacgt tccagccgat
     4441 cggcgccgag ctgcgctagc gcgtaggcct gatccttggc atgccggctg cggaagagat
     4501 ggacgccgtc gtcgcggcgc tcgatgcgcg cgta
//
LOCUS       DASUCJ010000868         1388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_85646, whole genome shotgun sequence.
ACCESSION   DASUCJ010000868 DASUCJ010000000
VERSION     DASUCJ010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85646"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1388
                     /gene="dnaE"
                     /locus_tag="VFU90_10350"
     CDS             <1..>1388
                     /gene="dnaE"
                     /locus_tag="VFU90_10350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5480224.1"
                     /translation="VAQIVTFGTMAARAAIRDAGRALGVPLPDVDRVAKLVPSGPGGL
                     TISDAIDRLPEIKTLYATRPEIRKLLDTAKEIEGLARNAGTHAAGVVISAGPLIDYAP
                     LVRFSDGGVNTQYDMEWVEKIGLLKMDFLGLRNLTVMDNAVKEIRRTVDPAFLLETIP
                     LDDAATYEMLSRGETMGVFQLESDGMKRVCAELKPSRFEDIIALVALYRPGPMELIPD
                     FIAIKHGRRKPKYLHPKLEPILAETYGIACYQEQVMQIARDVAGFSMSEADELRKVMG
                     KKQKEKIPVYREKFVRGAVETSGIGEQLAEDIFKFVEPFAGYGFNKSHAAAYGWIAYQ
                     TAYLKANHPLQYLTALMTSIKDKTEKLVEYIDEAKKLGIVVLPPDVNESLVDFAVVGD
                     RVRFGLAAVKGVGEGAVRSIIEARERGGRFADLFDLTKRVDFKQVNRRVFEALIKCGA
                     LDGLPGNRAQNLA"
BASE COUNT          289 a          395 c          461 g          243 t
ORIGIN      
        1 gtcgcgcaga tcgtgacgtt tgggacgatg gcggcgcggg ccgcgattcg ggacgccggg
       61 cgagcactcg gcgtgccgct gcccgacgtg gatcgggtcg cgaagctcgt cccctcgggt
      121 cccgggggtc tgacgatctc cgacgcgatc gatcggttgc cggagatcaa aacgctgtac
      181 gcgacgcggc cggaaattcg caagctgctc gacaccgcca aagaaatcga agggctcgcg
      241 cgtaacgcgg ggacgcacgc tgccggggtg gtgatttcgg cgggaccgct gatcgactac
      301 gctccgctcg tgcgcttcag cgacggcggc gtcaacacgc aatacgacat ggagtgggtc
      361 gagaagatcg gcttgctcaa gatggacttc ttggggctgc gcaatctgac cgtcatggac
      421 aatgcggtca aagaaattcg ccggacggtc gatcccgctt tcctactgga gacgattccg
      481 ctcgacgacg cggcgaccta cgagatgctg agccgcggcg agacgatggg cgtgttccag
      541 ctcgaatccg acggcatgaa acgggtttgc gcggagctca agccttcccg gttcgaagac
      601 atcatcgcgc tggtggcgct gtatcgcccg gggccaatgg agctgattcc ggatttcatc
      661 gcgatcaagc acggccgccg caaaccgaaa tatctgcatc cgaaactcga gccgattctg
      721 gcggagacgt acggtatcgc ctgttaccag gaacaggtga tgcagatcgc ccgcgacgtc
      781 gccggtttca gcatgagcga agcggacgaa ctgcgcaagg tcatgggaaa gaaacagaaa
      841 gaaaaaattc ccgtgtatcg cgaaaaattc gtgcgcggtg ccgtcgaaac gtcgggtatc
      901 ggcgagcagc tggccgagga tatcttcaag ttcgtggagc cgttcgccgg atacggcttc
      961 aacaagtcgc acgcggccgc ttacgggtgg atcgcctatc aaaccgcgta cttgaaggcc
     1021 aaccatccgc tgcagtacct aacggcgttg atgacgtcga tcaaggataa gaccgagaag
     1081 ctggtcgagt acatcgacga agcaaaaaag ctcgggatcg tcgtcctccc gccggacgtc
     1141 aacgagtcgc tggtagactt tgcggtggtc ggcgaccgcg tccggttcgg gctggcggcc
     1201 gtgaagggcg tcggcgaggg tgcggtgcgc agcatcatcg aagcgcgcga gcgcggcggc
     1261 cgtttcgcgg acctgttcga tttgacgaaa cgggtcgatt tcaaacaggt caatcgacgc
     1321 gtcttcgagg cgctcatcaa gtgcggagcg ctcgacggat tgccgggaaa tcgcgcacaa
     1381 aacctggc
//
LOCUS       DASUCJ010000869         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_666328, whole genome shotgun sequence.
ACCESSION   DASUCJ010000869 DASUCJ010000000
VERSION     DASUCJ010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666328"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFU90_10355"
     CDS             complement(<1..751)
                     /locus_tag="VFU90_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5480225.1"
                     /translation="MDYLPPPSTEEPSPKPARRRPAGVLGTAGAAILAFLLKFKLLLL
                     VGLKLLAPSWTFLLSLWIYVAIFGWRLAVVVMLLLLAHELGHYFAFRAYGLPARLPAF
                     VPLLGAFTAGAPPQNLEDDAYIALAGPLTGLALATVCLEAGNLANSRFWYACADLSAF
                     LNLFNMIPMPPSDGGRIAGALWPPLWVGGLVLFLAFAFFFHIPILFVVIVGVLGIPAM
                     LAAWHGRVDPRAAGMTIGARVRVSVWYLATLL"
     gene            811..2082
                     /locus_tag="VFU90_10360"
     CDS             811..2082
                     /locus_tag="VFU90_10360"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5480226.1"
                     /translation="MGGGFAGTAVAQRLERRLRPDEADIVLLSRENYTLFTPMLPEVT
                     SGELEVRHIVTPIRAQLSRTAFVLAEAFEIDVERRRVAYRHMLTGITVELVYDQLVLA
                     VGSSTSTFGLPGVAENVWALKTLDDADALRNHLVWLLELADTIADDRRRGELLTVVVV
                     GGGFTGVEAAGEIFELFRSVLRYYHRLRLGDVRMILVEGGATLLPGLPPKMGEYSRRV
                     LERRGIEVLTGDVVKSADERGLTLGSGRRIDSATVIWSAGVAPSQSVAKTALPKTRRG
                     AVVTGPDMRVAEFPNVWALGDCASIPDGKGGTFPMTAQHAIREGPHLADNLVRVLRGE
                     ATRPFRYDSLGMMAALGGRKAVAQLPGDRVITGFIAWFLWRTYYLLRLPGLDRRLRVA
                     FDWTLELLFPRDISELRVDTESATAGREPRP"
     gene            2094..>2369
                     /locus_tag="VFU90_10365"
     CDS             2094..>2369
                     /locus_tag="VFU90_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480227.1"
                     /translation="MELREPIERLIRDPKEFTREVRFPDPAEIRIYDETLRDGEQMPG
                     VCYTPPQKLEIAKALAAIGVHVMSVGFPAASQGDRRTLQLVMDAKRRG"
BASE COUNT          449 a          771 c          789 g          360 t
ORIGIN      
        1 cgagcagcgt cgcaagatac cagacgctca cccgtacccg tgcgccgatc gtcatgccgg
       61 cggcgcgcgg atcgacgcgg ccgtgccacg ccgccagcat cgcgggtatg cccaagacac
      121 ccacgatcac gacgaagaga atcggaatgt ggaagaaaaa cgcaaatgcg agaaacagca
      181 ccaagccccc gacccagagc gggggccaga gtgcgcccgc aatccgtccc ccgtcggacg
      241 gcggcatcgg aatcatattg aacaaattga gaaaggcgga cagatccgcg cacgcgtacc
      301 aaaagcggct gttggcgagg ttgccggctt ccaagcaaac cgtcgccagc gccaagccgg
      361 tgagcggacc ggcgagtgcg atgtacgcgt cgtcctcgag gttttgcggg ggagcgccgg
      421 ccgtaaacgc accgagcagc gggacgaacg ccggtaagcg cgccggaagt ccgtaggcgc
      481 ggaatgcaaa gtagtgaccg agttcgtggg caagcaggag aagcatgacc accaccgcga
      541 gccgccagcc gaagatcgcg acgtaaatcc acagcgacaa gagaaacgtc cagctcggtg
      601 cgagcagctt gagcccgacg agcagcagca gcttgaactt cagcaagaac gccaggatcg
      661 cggcgccggc ggtgcccaga acgccggccg gccggcggcg cgcgggcttg ggcgaaggtt
      721 cctcggtcga gggcggcggg aggtaatcca ccgcgctctc ttcaaccgca tccgctacga
      781 gtgcctggta aaaaacccgt catcgtcatc gtgggcggag gcttcgccgg cactgcggtg
      841 gcccaacggc tggaacgccg cttgcgcccc gacgaagccg acatcgtcct cctgagccgc
      901 gaaaactaca cccttttcac gccgatgctg ccggaagtga cctccggcga gctggaagtg
      961 cgccacatcg tcacgccgat tcgcgcgcag ctttcgcgaa cggcattcgt cctcgccgaa
     1021 gccttcgaaa tcgacgtcga gcggcggcgg gtcgcgtatc gccacatgct gaccggcatc
     1081 accgtcgagc tcgtctacga tcagctcgtg ctcgcggtag gctcgtcgac ctcgacgttc
     1141 ggtttgcccg gcgtcgccga aaacgtgtgg gcgctcaaga cgctcgacga tgcggacgcg
     1201 ttgcgcaatc atctggtctg gctgctcgag ctggccgata cgatcgccga cgatcggcgc
     1261 cgcggcgaat tgctgacggt cgtcgtcgtg ggcggcgggt tcaccggcgt cgaagcggcc
     1321 ggcgagattt tcgagctgtt ccgcagcgtg ctgcgctact accaccgttt gcggctcggc
     1381 gacgtgcgga tgattttagt ggagggcggt gcaacgctgc tgcccgggct cccgccaaag
     1441 atgggcgagt actcgcgccg cgttctggaa cggcgcggca tcgaagtgct caccggcgac
     1501 gtggtgaaga gcgccgacga acgcggcctc acgctcggca gcggtcggcg gatcgacagc
     1561 gcgacggtga tttggagcgc cggcgtagcc ccatcgcaat cggttgccaa aaccgcgctg
     1621 ccgaaaacca gacgcggcgc ggtcgtgacc ggccccgata tgcgcgtcgc cgaatttccg
     1681 aacgtttggg cgctcggcga ctgcgcttcg atccccgacg gcaaaggcgg gacgtttcca
     1741 atgaccgcac aacacgccat tcgcgaaggt ccgcacctcg ccgacaactt ggttcgcgtc
     1801 ctgcgcggtg aagccacgcg gccgtttcgt tacgactcgc tcggcatgat ggcggcgcta
     1861 ggcggccgca aggcggtcgc gcaacttccc ggcgatcgcg tcatcaccgg attcatcgcc
     1921 tggttcctct ggcggaccta ctatctgctg cgattgccgg ggctggaccg gcgcctgcgg
     1981 gtagccttcg actggacgct ggaactgctg ttcccgcggg atatttccga gctgcgcgtc
     2041 gacaccgaga gcgcgacggc gggccgggag ccccgaccgt agaagagcct catatggaac
     2101 tacgcgagcc gatcgagcgg ttgatccggg atccgaagga gttcacgcgc gaggttcgct
     2161 ttccggatcc ggcggaaatc cgcatctacg acgaaacgct gcgcgacggc gagcagatgc
     2221 ccggcgtctg ttacacgccg ccgcaaaagc tcgaaatcgc caaagcgctc gccgcaatcg
     2281 gcgtacacgt catgagcgtg ggcttcccgg ccgcgtcgca aggggatcgc cggacccttc
     2341 agctcgtgat ggacgccaag cgccgggga
//
LOCUS       DASUCJ010000870         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_895774, whole genome shotgun sequence.
ACCESSION   DASUCJ010000870 DASUCJ010000000
VERSION     DASUCJ010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895774"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VFU90_10370"
     CDS             complement(<1..1030)
                     /locus_tag="VFU90_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480228.1"
                     /translation="MPSRTSLVSIALWLAIALAGTPAHAAVIQTFGFPVARAPHPLAL
                     DPSLTDPAWLKGLVPSNGAWQNVTTRSPAGMSTTVYLLYDDQNLYVGFKNEQPGVPIT
                     AAQTTNDVGFGLDDFDGIGLDTSGAGSQSYYFETTPRGVRYEQSSENVRYRPRWNAAG
                     KIDGSSWNSVMIIPLDVLRVPKGGAQTWRLQFVRSVATRGEHISWVWDPIMQDSGAGN
                     WPTFADTRFWAAGSFTISSTAATKPKPRADIYGLASVGLDRNLAQQPNGTFLPENARW
                     YGADVSLPLTPTIRFVGTANPDFSNVEIDQQTIAPQEFQRQLVEYRPFFAQGAAFINA
                     ASGPRTPTG"
     gene            1110..1277
                     /locus_tag="VFU90_10375"
     CDS             1110..1277
                     /locus_tag="VFU90_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480229.1"
                     /translation="MRREEFLGSASAIVPAGASGADVAAPVERRGGRALSSATFRRGP
                     GSTGLPSGGGA"
     gene            1329..1712
                     /locus_tag="VFU90_10380"
     CDS             1329..1712
                     /locus_tag="VFU90_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480230.1"
                     /translation="MNRVVHFEIHAENPERAAAFYHGVFGWAITKWDGPTDYWLVDTG
                     EGNPGIHGGIVQREGGAPVPGAAVNAFPCTVDVQSLDATIASVEKNGGKLVVPKMEIP
                     GIGWLAYANDTEGNIFGMMQSTRSS"
     gene            complement(1796..2134)
                     /locus_tag="VFU90_10385"
     CDS             complement(1796..2134)
                     /locus_tag="VFU90_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5480231.1"
                     /translation="MVEQIRAAVERGELQPEMALPPVRQLAGDLGVAPNTVARAYAEL
                     QADGWLVGDGRRGTRVAGDVPRADGRERARTLRDALERVVASLQHRGFTQREISEELL
                     RLASSPGQQT"
     gene            complement(2188..>2483)
                     /locus_tag="VFU90_10390"
     CDS             complement(2188..>2483)
                     /locus_tag="VFU90_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480232.1"
                     /translation="ASLALCAIAWRVAVAPSILFGDDPQLEYLVDEHVRFCRANNLLG
                     LACAPPTVLAAIGNASLPNGSHFFDAVSLAVIVAFFVVMIISLNPLRKRLSFA"
BASE COUNT          436 a          733 c          917 g          397 t
ORIGIN      
        1 cgccggtcgg cgtgcgcgga ccggacgcgg cgttgatgaa ggccgcgcct tgggcgaaaa
       61 acggccggta ctcgaccagc tggcgctgaa attcttgcgg cgcgatcgtt tgctgatcga
      121 tttcgacgtt cgaaaaatcg ggattggcgg tgccgacgaa gcggatcgtc ggcgtcagcg
      181 gaagactcac gtcggcaccg taccagcgcg cgttttccgg taagaacgtc ccgttgggct
      241 gctgggcgag attgcgatcg agcccgacgc tggcaagccc gtagatgtcg gcgcgcggtt
      301 tcggtttggt ggcggcggtc gacgaaatcg tgaagctgcc ggccgcccag aaacgcgtgt
      361 cggcaaacgt cggccagttg ccggccccgg aatcctgcat gatcggatcc cacacccacg
      421 agatgtgctc gccgcgcgtc gcgacgctgc gcacgaactg taagcgccag gtttgcgcgc
      481 ctcccttggg gacgcgcaga acgtcgagcg ggatgatcat cacggagttc caggagctgc
      541 cgtcgatctt gccggcggcg ttccagcgcg gccggtagcg gacgttttcg ctggactgtt
      601 cgtagcgcac cccgcgcggc gtggtttcga agtagtacga ctggctgccg gcgccgctcg
      661 tgtcgaggcc gatgccgtcg aaatcgtcga gcccaaagcc gacgtcgttg gtggtctgcg
      721 cggccgtaat cgggacgccg ggctgttcgt tcttgaagcc gacgtagaga ttctgatcgt
      781 cgtagagcag gtagacggtg gtgctcatgc cggcgggcga acgggtagtg acgttttgcc
      841 acgcgccgtt gctggggacc aggcccttca gccaggcggg gtcggtcagc gacggatcga
      901 gcgccagcgg atggggcgca cgggccaccg gaaagccgaa ggtttggatg acggcagcgt
      961 gggccggcgt tcccgcgagg gcgatcgcga gccaaagggc gatggaaacg agcgaggtgc
     1021 gtgatggcat gaagaggctt gctcggccag agtctaacac tgggggaccg gggcgtcatc
     1081 aagggcgcga agtttgatgg attattgacg tgcgacgcga ggagtttctc ggctcggcaa
     1141 gcgcgatcgt gccggccggg gcgagcggcg ccgacgtcgc cgcgccggtg gagcggcggg
     1201 gcggccgagc gctgtcgagc gcaacgtttc gacgcgggcc ggggtcgacc gggttaccat
     1261 cgggcggcgg cgcgtagaac cgagtgcggc cgcgtagtta cccgcaatcg aaagaaaggg
     1321 ccagcctaat gaaccgcgtg gtgcactttg aaattcacgc cgagaacccc gaacgcgcgg
     1381 ccgcgttcta ccacggcgtg ttcggttggg cgatcaccaa gtgggacggc ccgaccgatt
     1441 actggctggt cgataccggc gagggcaatc cgggcatcca cggcgggatc gtgcagcgcg
     1501 agggcggcgc gccggtgccg ggggccgcgg tgaacgcgtt tccgtgcacg gtcgacgtgc
     1561 agtcgctcga cgcgacgatc gcgagcgtcg agaaaaacgg cggaaaactg gtcgtcccga
     1621 aaatggagat tccgggcatc ggctggctgg cgtacgctaa cgacaccgaa ggcaacatct
     1681 tcggaatgat gcagagcacg cggagttcgt aggaacgtat tttgagcgag gtgtcgcacg
     1741 acgacggcgc ttcctggccg tcttcgccgg atcgtttcgc taacccgagc gggctttacg
     1801 tctgctgacc cggtgaacta gcaaggcgca gaagctcttc ggaaatttcg cgttgcgtga
     1861 agccgcggtg ctgcagcgaa gcgacgacgc gttcgagcgc gtcgcgcagc gtgcgcgcac
     1921 gttcgcggcc gtcggcgcgc ggaacgtcgc cggcaacgcg cgtgccgcgg cggccgtcgc
     1981 cgacgagcca gccgtcggct tgcagttcgg catacgcgcg tgcgaccgtg ttgggcgcga
     2041 cgccgagatc gccggcgagc tgacgcaccg gcggcaacgc catttccggc tgcagctcgc
     2101 cgcgttcgac cgcggcgcga atttgttcga cgatctgacg gtacggcggg acgtccaacg
     2161 tttcgtcgac gacgagatac gaaccgctca cgcgaaggag aggcgcttgc gcagcggatt
     2221 gaggctgatg atcatcacga cgaagaacgc gacgatgacc gccagcgaga cggcgtcgaa
     2281 gaagtgcgaa ccgttcggga gcgaggcgtt accgatcgcc gccagtaccg tcggcggcgc
     2341 gcacgcgagg ccgagcagat tgttcgcgcg gcagaagcgg acgtgttcgt cgaccaagta
     2401 ttcgagttgc ggatcgtcgc cgaagaggat cgagggggcg accgccacgc gccaggcgat
     2461 cgcacacagc gcgagcgacg cgg
//
LOCUS       DASUCJ010000871         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1140471, whole genome shotgun sequence.
ACCESSION   DASUCJ010000871 DASUCJ010000000
VERSION     DASUCJ010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140471"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFU90_10395"
     CDS             complement(<1..600)
                     /locus_tag="VFU90_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480233.1"
                     /translation="MLDRGHLWLLAIAIAVWPVSVRAQTAPAPKPTSTPLSVSGFFRS
                     YYFTRQNATNNPGVQFDYSTKKCNGAGPCVNQATLNSAIDLHADYEFGGGWYAGGSYF
                     YAQPFNGPCSPASQHAKDDPCVSQDPPNTNPDDTLPGFALSTFPEAYLGYKRAGFSAK
                     AGDQLFDSPWAAPYDGSRLKPAAYQGADFEYDWGSGWRAD"
     gene            709..1407
                     /locus_tag="VFU90_10400"
     CDS             709..1407
                     /locus_tag="VFU90_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480234.1"
                     /translation="MTVTDAVRYVSLPIVVALCACGFGSEVRQQVHQTIPAAAAPAIH
                     LENAAGSVQIDAWSKSVIDLKATKSANTLEALRNVQIDVRTGNDGVFITTKYAGASHE
                     GGVSYVLTVPANSSLDVRNGAGSVGITGVRGSVAVTTQAGSITATLGTVAGSRSVVLG
                     ATTGSIRLFVGSGSSARVQARSSVGSVSTGFPGVSVTRENVVGATGSGKIGAGSATIR
                     LTTTTGSIALQRAP"
     gene            complement(1409..2740)
                     /locus_tag="VFU90_10405"
     CDS             complement(1409..2740)
                     /locus_tag="VFU90_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480235.1"
                     /translation="MRYFAPLALAAGWLLAACSTPPQATPPPAGSVARAAATGAGKIK
                     HVIIIVQENRSFDNLFQGYPGADTVSSGKNSQGQTIALQPESLTKAYIIDHSAKSMFA
                     ACDGTGKLPGTQCRMDGFDLEPSSGGPPNPQYVYVPHVESKPYFDMAAEFVLADRMFQ
                     SQLDESFTSHQYLIAAQAQSSVDLPKTYWGCDGGPSDTVQTIAQSRQYASPQPACFDY
                     QTLGDELDQAHLTWRFYTSRYTQPGGGGGEWSAYQAIRHIRYGADWNKDVVTPQKRFI
                     KDVGEGHLKDVTWITPICTNSDHVNCGGGYGPSWVTSLVNAAGESKLWNSTAIFVLWD
                     DWGGLYDHVPPPHADYDGLGFRVPLIVISPYAKKNYVSHVQYEEGSILRFVEDIFGLS
                     QLGASDTRANSPAGDCFDFNQPPRTFIPIQAPKGPAFFMSQPNDLRPPDDQ"
BASE COUNT          489 a          913 c          943 g          534 t
ORIGIN      
        1 atcggcccgc cagccggaac cccagtcgta ttcgaagtcg gccccttgat atgcggccgg
       61 tttgaggcgc gatccgtcgt atggcgcggc ccacggtgaa tcgaacagct ggtcgccggc
      121 cttcgcggag aaaccggcac gcttgtagcc gagatacgcc tccggaaagg tgctgagcgc
      181 gaaaccgggc agcgtgtcgt cgggattggt gttcggcgga tcctgcgata cgcacggatc
      241 gtctttggcg tgctgcgacg ccggcgaaca cggcccgttg aacggctggg cgtaaaaata
      301 cgagccgccg gcgtaccacc cgcccccgaa ctcgtaatcg gcgtgcaggt cgatggcgct
      361 attgagcgtt gcttggttca cgcacggccc ggcgccgttg cattttttcg tcgagtaatc
      421 gaactgcacg ccggggttat tggtcgcgtt ctgtcgcgtg aagtagtacg aacgaaagaa
      481 tccgcttacc gacaggggcg tgctggtcgg cttcggggcc ggagccgttt gcgcgcgaac
      541 gcttaccggc cacacggcga tcgcgatcgc cagcagccag aggtgcccgc ggtcaagcac
      601 gttgccgtcc ctcggccatt cgaccggcta ctccctttgg agcgcgcgta cctcccgcgc
      661 gaggcgcgct cgaccggacg tttggaattg gtgtaaggta cgaatagcat gaccgtcacg
      721 gacgccgttc gctacgtttc gctcccgatc gtcgtcgcgc tgtgcgcgtg cggcttcggc
      781 tccgaagtgc gtcagcaggt acatcagacg attccggccg ccgccgcgcc cgcaattcat
      841 ctcgagaacg ccgccggctc ggttcaaatc gatgcttgga gcaaatccgt catcgatttg
      901 aaagccacca agtccgcgaa cacgctcgag gcgctgcgca acgtgcaaat cgacgtccgc
      961 accggaaacg acggcgtttt catcactacg aagtacgccg gcgcttcgca cgaaggcggc
     1021 gtaagctacg tgctgaccgt tccggcgaac tcatcgctgg acgttcgcaa cggtgccgga
     1081 tccgttggga taaccggcgt tcgcggcagc gtcgcggtca cgacgcaggc cggcagcatc
     1141 accgcgacgc tcggcacggt agccggctcg cgatcggtcg tgctcggcgc gacgaccgga
     1201 tcgatccggc tgttcgtcgg ctccggcagc agcgcgcggg tccaagcacg cagctcggtc
     1261 ggttccgtga gcaccggctt tccgggcgtg agcgtgacgc gcgagaacgt cgtcggtgcg
     1321 accgggagcg ggaagatcgg tgccgggagc gcgacgatcc ggctgacgac cacgaccggt
     1381 tcgatcgcgc tgcagcgcgc cccctaactc attggtcgtc cggcggccgt aaatcgttgg
     1441 gctgcgacat gaagaaagcc ggacctttcg gcgcttgaat cgggatgaag gttcgcgggg
     1501 gttgattgaa gtcgaagcag tcgccggccg gggaattcgc gcgggtgtcg ctcgcaccca
     1561 gctgcgacaa accgaagatg tcctcgacga accgtaagat gctgccttct tcgtactgaa
     1621 cgtgcgaaac gtagtttttc ttcgcgtacg gtgagatcac gatcagcggt acccggaagc
     1681 ccagcccgtc gtagtcggca tgcggcggcg gcacgtggtc gtacagacct ccccagtcgt
     1741 cccacagcac gaagatcgcg gtcgagttcc acagcttgct ctcgcccgcg gcgttcacca
     1801 gcgacgtcac ccacgatggc ccgtaaccgc cgccgcagtt gacgtgatcg gagttggtgc
     1861 aaatcggcgt gatccaggtc acgtctttga gatggccttc cccgacgtcc ttgatgaagc
     1921 gcttctgcgg ggtgacgacg tctttgttcc agtccgcccc gtagcggatg tggcgaatcg
     1981 cttgatacgc cgaccactcg ccgcccccgc cgccgggctg cgtatagcgg ctggtgtaaa
     2041 accgccacgt caagtgcgcc tggtccagct cgtcgcctag cgtctgatag tcgaaacagg
     2101 ccggttgcgg cgatgcgtac tggcgcgatt gcgcgatcgt ttgaacggtg tcgctgggcc
     2161 cgccgtcgca gccccaatac gtcttgggaa ggtcgacgct cgactgggcc tgcgcggcga
     2221 tcaggtattg atgcgacgtg aagctctcgt ccagctgcga ttgaaacatc cggtcggcca
     2281 gcacgaactc ggcggccatg tcgaagtacg gcttcgattc gacgtgcggc acgtagacgt
     2341 actgcggatt gggcggcccg ccggacgatg gctccaagtc gaacccgtcc atccggcact
     2401 gcgtgccggg aagtttcccg gtaccgtcgc aggcggcgaa catcgacttc gcggagtggt
     2461 cgattatgta ggccttggtc aaactctccg gctgcagcgc gatcgtctgc ccctgcgagt
     2521 tctttccgct cgaaaccgtg tccgcgccgg gatagccctg aaacaggttg tcgaagctgc
     2581 ggttttcttg aacgatgatg atgacgtgtt tgatcttccc ggccccggtc gcagcggcgc
     2641 gcgccacgga gccggccggc ggcggcgtcg cttgcggcgg cgtcgagcag gccgcaagca
     2701 gccatcccgc cgcaagagcc agcggtgcaa agtatcgcat gtgcgagcct gctttcaact
     2761 cggcgttcgg ggtgtcatgc tacggaatcg gcgcgccctt cgtctcgggg gcgaatcgaa
     2821 gcgcgacggc taatccgagc gcgctcagcc cgcacgcacc gatcacgatg ttcgtcgcc
//
LOCUS       DASUCJ010000872         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_635769, whole genome shotgun sequence.
ACCESSION   DASUCJ010000872 DASUCJ010000000
VERSION     DASUCJ010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635769"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFU90_10410"
     CDS             <1..809
                     /locus_tag="VFU90_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU5480236.1"
                     /translation="TGWLGRYLDEADLPEKDLFKAVAVSQVLPEVLVANRTDVPTISN
                     VAQYGLIADRDPMARQAFSQDSRDRRLPFDSPYLQHVMQIEDHAQRSSEELPKLISGY
                     KPQAQYPPTPIGRSLALAAQIIGSDLGTKVLYVSHGSFDTHVSQTATQNNLLEQFSNA
                     IAAFYEDLAAHGNDRRVLTLTFSEFGRRIAENAARGTDHGEASPLFLIGGGVKGGVYG
                     TAPDLTATNMGNLRYTTDFRSVYATVLERWLGRPSSAVLSGSFEKLPVLG"
     gene            869..1282
                     /locus_tag="VFU90_10415"
     CDS             869..1282
                     /locus_tag="VFU90_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5480237.1"
                     /translation="MRPSSQRVVSFGKARVTGLPPKGTPKNYLMAWLLVMLAEKSLHG
                     YEIAKELNEKFEVITDAGSVYRALRQLERDGYISSWWNPSGQGPARRFYALTQPGRGA
                     LRAWSEALEQYRSNLETFFKLYERRREVTAGRKGA"
     gene            1311..2093
                     /locus_tag="VFU90_10420"
     CDS             1311..2093
                     /locus_tag="VFU90_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5480238.1"
                     /translation="MFLGSGGARFVVARQLRASGGMWMRFGETQIHVDPGPGALVRAL
                     AHVPPCNPRELDAIVLSHKHLDHSSDVNALVEAMTSGGFRRRGALFAPADAFDHEPVV
                     LPYVRRFVERLERLEEDAGPYAIGGVELRTSIAHLHAVLTYGLHFHYDGLRVAYVPCG
                     RYFEGLGAGYARLKPDVLIVNVLRYRDEMRVDHLTWDDARRVVAEVRPRVAIFQHFGT
                     KMLEASPARLAQELEDELGLRAIAAYDGLEVDLDAVAAVASD"
BASE COUNT          348 a          707 c          698 g          355 t
ORIGIN      
        1 acaccggctg gctcggccgc tatctcgacg aggcggatct gcccgaaaaa gacttgttca
       61 aggcggtcgc cgtttcgcag gtgcttcccg aggtgctggt ggccaaccgc accgacgtgc
      121 cgaccatttc gaacgtcgcg cagtacgggc tgatcgccga ccgcgacccg atggcgcggc
      181 aggcgttttc gcaggattcg cgcgaccggc gactgccgtt cgactcgcct tacctgcagc
      241 acgtcatgca gatcgaggat cacgcgcagc gcagctccga ggagttgccg aagctgatct
      301 cgggttacaa gccgcaagcg cagtacccgc ccaccccgat cgggcgcagc ttggccctgg
      361 cggcgcagat catcggcagc gatctcggca cgaaggtgct ctacgtttcg cacggctcgt
      421 tcgacaccca cgtgagccag acggcgaccc agaacaacct gctcgagcag ttctcgaacg
      481 cgatcgccgc cttctacgaa gatttggcgg cgcacggaaa cgaccggcgc gtgctgacgc
      541 ttacgttcag cgagttcggc cggcgaatcg ccgaaaacgc cgcgcgcggc accgaccacg
      601 gagaggcctc gccgctgttt ctgatcggcg gcggcgtcaa gggcggcgtc tacggaaccg
      661 cgcccgacct gaccgcgacg aacatgggca acctgcgcta cacgaccgat tttcgcagcg
      721 tatacgcaac ggttctcgaa cggtggctcg gccgtccgtc gtcggccgtg ctgagcggct
      781 cgttcgaaaa actccccgtc ctgggttgac ccgccggaag gccccgcgcg ccacaggtgc
      841 cgcttcgacc gcggtgcaag taggccgcat gcggccgagc tcccagcgcg tggtgtcctt
      901 cggcaaggcc cgcgtaaccg gtctcccgcc caaggggacg cccaagaact acttgatggc
      961 gtggctgctg gtcatgctcg ccgaaaaaag cctgcacggc tacgagatcg ccaaagagct
     1021 caacgagaaa ttcgaagtca tcaccgacgc cggctcggtc tatcgagcct tgcgccagct
     1081 cgaacgcgac ggatacatct cgtcctggtg gaatccgagc ggacagggtc cggcgcgccg
     1141 cttctacgcg ctcacgcagc ccggacgcgg cgcgctgcgc gcgtggagcg aggcgctcga
     1201 gcagtaccgc agcaacttgg agacgttttt caagctctac gagcgccggc gtgaggtgac
     1261 tgccggccgg aaaggcgctt aggcctccaa tccgtcaccc gtcctcgatt ttgtttctcg
     1321 gcagcggcgg cgcgcgcttc gtggtcgcgc gtcagctgcg cgcgtccggc gggatgtgga
     1381 tgcgcttcgg cgagacgcag attcacgtcg atcccggccc cggcgcgctg gttcgcgcgc
     1441 tcgcgcacgt tccgccgtgt aatccgcgcg agctcgacgc gatcgtgctc tcgcacaagc
     1501 acttggacca ctcgagcgac gtcaacgcgc tggtcgaagc gatgacgtcg ggcggttttc
     1561 gccggcgcgg cgcactgttc gcgccggccg acgcgttcga tcacgagccg gtggtcctgc
     1621 cctacgtgcg gcggttcgtc gaacggctcg agcggctcga agaagacgcc gggccgtacg
     1681 cgatcggcgg ggtcgagctg cgaacctcga tcgcacattt gcacgccgtg ctcacgtacg
     1741 gactgcattt ccattacgac ggcctgcggg tcgcttacgt gccgtgcggg cgttatttcg
     1801 aaggccttgg cgccggctac gcgcgcctga agcccgacgt cctgatcgtc aacgtgctgc
     1861 gctatcgcga cgagatgcgg gtcgaccacc tcacctggga cgacgcccgc cgggtcgttg
     1921 cggaagtccg cccgcgcgtc gcgatcttcc agcacttcgg cacgaaaatg ctggaagcga
     1981 gcccggcaag gctcgcgcag gagttggaag acgagctcgg cttgcgggcg atcgcggctt
     2041 acgacgggct ggaagtcgat ctcgatgcgg ttgcggcggt tgcctccgat tgagttcgcg
     2101 caggtgcg
//
LOCUS       DASUCJ010000873         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1170837, whole genome shotgun sequence.
ACCESSION   DASUCJ010000873 DASUCJ010000000
VERSION     DASUCJ010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170837"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VFU90_10425"
     CDS             complement(<1..1088)
                     /locus_tag="VFU90_10425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5480239.1"
                     /translation="MTRQDFDVVVIGAGPAGTAAALRAADLGAKTALVTRGAFGGMSA
                     NEGPIPVRTLAHAARLAREARQLPRYGISVGTPSLDYDRLIARAREVVDEARGNSTLL
                     QQVRSVGVALFENSGTAGFDDAHTVASERGPLLRGNSVVVCSGGVSRALDVPGAELTV
                     TPADAFGLRQLPESLLVVGAGATGMQVASIFNDLGSKIVLCQTGPRIAPSEDAEVSIE
                     LARAYREHGIDVHENCGPIERFEAIDGRVRTVFRSREPVETQLVVSCIGWVADTERLN
                     LRAAGVRTSSRGFIEVDDYRRTSASHVFAAGDVIGRSMLVPQAVRDGYVAGGNAATGK
                     LFPVPGGLEPIGSFTDPEYAKVGLTEDEA"
     gene            complement(1085..2002)
                     /locus_tag="VFU90_10430"
     CDS             complement(1085..2002)
                     /locus_tag="VFU90_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480240.1"
                     /translation="MTCAGVALAALSCAGQSAVPSPSPGASTPLRPALSDPCGGSQEL
                     LKKYLSASPCVFVRGQASLQMTYSGTNVPVVVSEGPQASQVVESSHAFGYPGALLNVG
                     VTPTSQVTIVLPSFSQISSTQKGVTAGASDVEFRYKQFISVDRKRGILTGLLLTYEAP
                     TGSPGLAAPGPAYEINPLVNVALNGARTIAANFSFPVSNATNSDGVARSWSFAPQGVV
                     VWRSPGGTLLAGIVQYAFETHSTYLTFNAAQLLSRNFQVQGTYGGNDATVDYVNPIQD
                     VAQARATVYSRSFTIGISYLVGNSEPLSP"
     gene            2193..>2667
                     /locus_tag="VFU90_10435"
     CDS             2193..>2667
                     /locus_tag="VFU90_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480241.1"
                     /translation="MSTQEHDWTKPQAMAIPKEGYFEFQQGNYGPIFPRTPACYGFTI
                     IAKLKPGREPAMREYAAVVEKALENDPNYLEALKLHYLKWVLFDIGKETYFMYQGIFD
                     TDFDKYTDDAVALFQKAGLSTAFENLEGFPEDWRTNVPAFIKFVREHHRPSFMEYG"
BASE COUNT          515 a          869 c          803 g          480 t
ORIGIN      
        1 gcctcgtcct cggttaggcc gaccttcgcg tattcgggat cggtgaaact gccgatcggt
       61 tcgagaccgc ccggaaccgg aaagagcttt ccggttgcgg cgttgccgcc ggcaacgtag
      121 ccgtcgcgca cggcctgcgg caccagcatc gaccgcccga tgacgtcgcc ggcggcaaac
      181 acgtgcgagg cgctcgtacg ccgatagtcg tcgacttcga taaaaccccg cgacgaggtg
      241 cgtacgcccg ccgcgcgcag attcaaccgt tccgtatcgg ccacccagcc gatgcagctt
      301 acgaccagct gggtttccac cggttcccta ctgcgaaaga ccgttcgcac gcggccgtcg
      361 atcgcttcga atcgctcgat tggcccgcag ttttcgtgca cgtcgattcc gtgttcgcgg
      421 tacgcacgcg ccaactcgat cgaaacctcg gcatcctcgc tcggggcgat ccgcggtccg
      481 gtttgacaca gcacgatttt cgaacctaag tcgttgaaaa ttgaagccac ttgcattccg
      541 gtcgcgcccg cgccgacgac cagcagcgat tcgggaagct gcctcaagcc gaacgcgtcg
      601 gcgggcgtca ccgtgagttc cgcgcccggt acgtccagcg cgcggctgac cccgccggag
      661 caaaccacga cgctgttgcc gcgcagcagc ggtccgcgtt cgcttgcaac ggtgtgcgcg
      721 tcgtcaaatc cggccgttcc gctattctcg aacagcgcca cccccactga gcgcacctgc
      781 tgcaacagcg tcgaattgcc gcgcgcctcg tcgacgactt cccgcgcccg cgcgatgaga
      841 cgatcgtaat cgagcgatgg agttcccacc gagatgccgt agcgcgggag ctgccgcgct
      901 tcccgcgcga gtcgcgcagc gtgcgcgagc gtgcgaaccg gaatcggccc ttcgttggcc
      961 gacattccgc cgaaagcgcc acgtgtcacg agcgcggttt ttgctccaag atcggctgcc
     1021 cgaagagccg ccgccgtgcc ggccggcccg gccccgatga cgacgacgtc gaagtcctgg
     1081 cgcgtcatgg cgagagcggc tcgctgttcc cgacgagata gcttatgccg atcgtaaacg
     1141 agcggctgta aaccgtcgcc cgcgcttgcg ccacatcttg gatcgggttg acgtagtcga
     1201 ccgtggcgtc gttaccgccg taggtacctt gcacttggaa attcctggaa agcagctgcg
     1261 ccgcattgaa cgtcagatac gtcgaatgcg tttcgaacgc gtactgcacg atgcccgcga
     1321 ggagcgtacc accgggcgaa cgccacacga cgactccctg cggcgcgaaa ctccaagacc
     1381 gggcgactcc gtcggagttc gttgcgttcg agaccggaaa ggagaaattc gcagcaatcg
     1441 tccgcgctcc gttgagagcg acgttgacga gcgggttgat ctcgtaggcg gggcccggcg
     1501 ccgcgagtcc cggcgatccc gtcggcgctt cgtacgtcag cagcagcccg gttagaattc
     1561 cgcgcttacg gtccacggag atgaactgct tgtaacggaa ttcgacgtcg gatgcgccgg
     1621 ccgtcacgcc tttctgcgtg gaggatatct gcgagaacga cggcagcacg atcgtgactt
     1681 gcgaggttgg cgtgacgccg acgttgagca aggcgcccgg atagccgaaa gcgtgactcg
     1741 actcgacgac ttggctggct tgcggacctt cggaaacgac gacgggaacg ttcgtccctg
     1801 aataggtcat ttggaggctc gcttgacccc tcacgaagac gcagggcgaa gcggagagat
     1861 atttcttcaa aagctcctgg ctgccgccgc acggatcgct caacgccggc cgcagcggcg
     1921 tgcttgcacc gggagacggc gaaggaaccg ccgattgccc cgcgcacgat agcgccgcga
     1981 gcgcgacgcc agcgcacgtc acgttcacgc tccgagcctg cgtcgcaatc cggcaagccc
     2041 cataacattg aattctacca cccgctgcgc gccccgaaca ccctcgaaac gagtccttta
     2101 cggtcccatt tcattttgtt gggcttcacc ataggtagcg gcccggtgtg aggtgaactc
     2161 tcgcggtccc catcggcaaa ggagttgagc gtatgtccac gcaggagcac gactggacga
     2221 aacctcaggc gatggcgatc cccaaagagg gatatttcga atttcagcaa ggcaactacg
     2281 gcccgatctt cccgcgaacg cccgcctgtt acggcttcac gatcatcgcg aagttgaagc
     2341 ccgggcgcga acctgcgatg cgcgaatacg ccgccgtcgt cgaaaaggcg ctcgagaacg
     2401 accccaacta tctcgaagct ctcaagctgc actacttgaa atgggtgctt ttcgatattg
     2461 gaaaagagac ctatttcatg taccaaggca ttttcgatac ggatttcgat aagtacaccg
     2521 acgatgcggt cgcgcttttc cagaaagccg gcctcagcac ggcattcgag aatctcgaag
     2581 gtttccccga ggactggcgg accaacgtgc cggccttcat caagtttgtt cgcgaacacc
     2641 atcgcccgag cttcatggag tacggcg
//
LOCUS       DASUCJ010000874         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_635788, whole genome shotgun sequence.
ACCESSION   DASUCJ010000874 DASUCJ010000000
VERSION     DASUCJ010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635788"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..>1196)
                     /locus_tag="VFU90_10440"
     CDS             complement(107..>1196)
                     /locus_tag="VFU90_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEU5480242.1"
                     /translation="NVYLDAAPKAPDLAPAELSASQGFGNTMKALAFMYVIETRAGLG
                     APVDVDRPVTAPPADFVSEDSVYGYIIGLLDSAQTALQNAGGTSFPFPVPPGFTGFDQ
                     PSTFLLFNRALKAKAEILRGTAGCGQPCFQAALTTLPQSFIDPTPSLLGIGPRFDFST
                     ASGDQQNGLAEPLDGTTFFADTFMLVHAQRQAGGELDARVLAKTARATRDPIWSIPAV
                     PLPGVLKYVIYFTGGQPDLSKPVPIIRDEELILLRAEAEIQTGDLTDALTDINFIRTN
                     SGRLPAISSGTWTSMSNADRINELLYNRWFSLMWEQGTRWIDARRYNLLGTIEPGVPG
                     GQVPQRMPIPQDECSARNLPTNCDPLGT"
BASE COUNT          202 a          361 c          417 g          216 t
ORIGIN      
        1 gtgtccgtcg tgtccggtcg tgtccgtctt caaatgagcg tatcacgatg cacgacggcg
       61 gggaccgttc ggcccccgcc gtcgccactg cgcgtccagt tcgcgatcag gtgcctaacg
      121 gatcgcaatt ggtgggcagg ttgcgtgcgc tgcactcgtc ctgcggaatc ggcatgcgct
      181 gcggaacctg accgcccgga actcccggct cgatggtgcc gagcaggttg tagcgtcgtg
      241 catcgatcca gcgcgtgccc tgctcccaca tgagtgagaa ccagcggttg tagagcaact
      301 cgttgatgcg atcggcgttg gacatggacg tccaggtgcc ggacgagatc gccgggagac
      361 gtccggaatt cgttcggatg aagttgatgt ccgtgagcgc gtcggtgaga tcgccggttt
      421 ggatctcggc ctccgcacgc aacaagatca actcttcgtc gcgaatgatc ggaaccggct
      481 tgctgagatc gggctggccg ccggtgaaat aaatgacgta cttgagcacg cccggcagcg
      541 gcacggcggg aatcgaccag atgggatcgc gggtcgcgcg cgccgtcttg gcgaggacac
      601 gagcgtccag ctcgccgccg gcctgacgtt gcgcgtgcac cagcatgaac gtgtcggcga
      661 agaacgtggt gccatcgagc ggctcggcga ggccgttctg ttggtcgcct gatgcggtcg
      721 agaaatcgaa ccgcggcccg atgcctaaca acgatggcgt cggatcgatg aaggattgcg
      781 gcagcgtggt cagcgcggcc tggaagcacg gctggccgca ccccgccgtt ccccgcagga
      841 tctcggcttt cgccttgagc gcgcggttga acaggaggaa cgtggatggt tggtcgaatc
      901 cggtgaatcc cggcggaacc gggaacggga acgacgtacc gcctgcgttc tggagcgcgg
      961 tctgcgcgct gtccagcaac ccgatgatgt agccgtagac gctgtcttca ctcacgaaat
     1021 cggccggcgg cgccgtcacc ggacgatcga cgtcgaccgg tgcgccgagg cccgcgcgcg
     1081 tttcgatgac gtacatgaac gcgagcgctt tcatcgtgtt gccgaaccct tgcgacgccg
     1141 agagctcggc gggcgcgagg tccggcgcct tcggcgctgc gtcgaggtag acgttg
//
LOCUS       DASUCJ010000875         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_9402, whole genome shotgun sequence.
ACCESSION   DASUCJ010000875 DASUCJ010000000
VERSION     DASUCJ010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9402"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="secF"
                     /locus_tag="VFU90_10445"
     CDS             complement(<1..974)
                     /gene="secF"
                     /locus_tag="VFU90_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5480243.1"
                     /translation="MLFRRLNWNIVGWFRIVTTISYAVIAVGIGSMFYHAFQGGQGFQ
                     PAHLLRLGLSFTGGTDIAVRFTQPTTTDQVKNALAGLGLTDESVTTAGSDGKGFQIQT
                     QTAFANDSSPMWNALGTVAPVDRAASSITAVGPSLGREYLVKALWALVIALGIQFLYI
                     AFRFGWNYIFGLVTVIALVRDAAMMIGIYSLADRRADDAFLAAVLTVIGYSVMDTIVI
                     LDRIRENTKLMAGSPYDVIVNESIKQTMTRSFNTLATVVITLVALLTLGGASLKNFAF
                     ALLVGIVSGGYHSIFFSAPLVLHFRKRQLEASTRRRQAGFTTDRTVTAR"
BASE COUNT          228 a          357 c          362 g          180 t
ORIGIN      
        1 cgtgccgtga cggtgcggtc ggtggtgaaa cccgcctgcc ggcggcgggt cgacgcctcg
       61 agctgccgtt tgcggaaatg gagcaccagc ggcgccgaga agaaaatcga gtgatagccg
      121 cccgaaacga taccgaccag cagcgcgaac gcgaagttct tcaagctcgc gccgcctaac
      181 gtcagcagtg cgaccagcgt gatcacgacg gtagccagcg tgttgaacga gcgcgtcatc
      241 gtctgcttga tcgactcgtt cacgatcacg tcgtagggcg agcccgccat gagcttggtg
      301 ttctcgcgga tccggtcgag gatgacgatc gtatccatca ccgagtagcc gatgacggtc
      361 agcaccgccg ccaaaaacgc gtcgtcggcg cggcggtcgg ccagcgagta gatgccgatc
      421 atcatcgcgg cgtcgcgcac cagcgcgatg accgtcacca aaccgaagat gtagttccag
      481 ccgaaccgga acgcgatata caggaactgg atgccgagcg cgatgaccag cgcccacaac
      541 gctttgacga gatactcgcg gcccagcgac ggaccgacgg cggtaatcga agaggcggcg
      601 cgatcgaccg gagcgaccgt tccgagcgcg ttccacatcg gcgaggagtc gttggcgaac
      661 gccgtttgag tctgaatttg gaagcccttg ccgtcgctcc cggcggtggt taccgattcg
      721 tcggtcagtc ccagtcccgc cagcgcgttc tttacctgat cggtcgtcgt cggctgcgtg
      781 aagcgcaccg caatgtcggt gcctccggtg aacgacaggc cgagccgcag cagatgcgcc
      841 ggctggaatc cctgaccgcc ttggaaggcg tggtagaaca tcgagccgat gcccaccgcg
      901 atgacggcgt acgagatcgt cgtgacgatc cggaaccagc cgacgatgtt ccagttgaga
      961 cggcgaaaca gcatgcgcta cgcccccgcc ttcgcgaaaa tgccgacgtc gtcgcgcttg
     1021 acgccgtaca gctccggcgc ggtcgcgacg ttgttgtcga ccagcgcgtc cacgaagaac
     1081 cgcgtgacga acacggccgt gatcagcgag acgacggttc cccaaaa
//
LOCUS       DASUCJ010000876         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1002719, whole genome shotgun sequence.
ACCESSION   DASUCJ010000876 DASUCJ010000000
VERSION     DASUCJ010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002719"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU90_10450"
     CDS             complement(<1..306)
                     /locus_tag="VFU90_10450"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5480244.1"
                     /translation="MSNQVSVNASEETIGLGPVAVRFLLTGSDTGGSMSIFEMTVPAG
                     QKIPAPAHTNDAYEETLYGIDGVLTWTIDGTAFPIGPGQAVCIPRGAVHRFDNFGDVA
                     "
     gene            369..815
                     /locus_tag="VFU90_10455"
     CDS             369..815
                     /locus_tag="VFU90_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5480245.1"
                     /translation="MSRPRLTLRARRASSDRAAPPVPSLIGALLRVPAQAIQRRLIAG
                     LNASGFSELRLPHMAVLQYPGPDGCRPLELAFRAGISKQAMNQLLKSLEQHGYVTRED
                     ANDDLRSRRVQLTKRGRAVSKRMIELLEVIETEWRGALGAYRFDEL"
     gene            complement(847..1095)
                     /locus_tag="VFU90_10460"
     CDS             complement(847..1095)
                     /locus_tag="VFU90_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480246.1"
                     /translation="MIDARTGKVQERLEMPAAVAVSGLESDGGDRFFCGGGSSGKVRV
                     VRRPARHASPRDDANPAARCASQGDDASSAEQQARSRG"
     gene            1219..1506
                     /locus_tag="VFU90_10465"
     CDS             1219..1506
                     /locus_tag="VFU90_10465"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEU5480247.1"
                     /translation="MQFARSPIGSTRLVADIATALRHGVRLGGHCVRSCAGLTTILLV
                     TGFMDLRVMALVTIAITAERLLPPRWRVAQAIGCVAVVVGIAMIGRAVGSG"
     gene            complement(1536..1949)
                     /locus_tag="VFU90_10470"
     CDS             complement(1536..1949)
                     /locus_tag="VFU90_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5480248.1"
                     /translation="MAARVDVYVDTSAFIAFADRSDTWHGTFLRLFATPPALITSSLV
                     VAEGHAWFLRRYDRGRALQFLAMIDAMSPLEIASVGKIEQAAASQLLRRFHDQDLTLT
                     DAVGLHLMHVRKLHTCWSTDFHLGLTGVPLVIQPH"
     gene            complement(1933..>2141)
                     /locus_tag="VFU90_10475"
     CDS             complement(1933..>2141)
                     /locus_tag="VFU90_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480249.1"
                     /translation="SLTEEELDAARREAARLGISLAELLRRSLRSILPLDATRPWMRY
                     AGFVESGDPKSSQHIDDIVYGRKG"
BASE COUNT          364 a          699 c          703 g          375 t
ORIGIN      
        1 cgcgacgtcg ccgaagttgt cgaagcggtg tacggcgccg cgcgggatgc agacggcctg
       61 acccggtccg atcggaaacg ctgtgccgtc gatcgtccag gtcagcacac cgtcgatccc
      121 gtacagcgtc tcctcgtagg cgtcgttcgt gtgcgccggg gcgggaattt tctggccggc
      181 aggcaccgtc atctcgaaga tcgacatact tccgccggtg tcgctgcccg tcagcaggaa
      241 ccgcacggcg accgggccga ggccgatcgt ctcttccgaa gcgttgaccg acacctggtt
      301 tgacatcgct ctcctccttc agtaaagttt gtttaccgtc gataaaacgg tacatcgcgt
      361 ttactaacat gtcaagacct cggctcaccc tgcgggcgcg gcgggcctcc tccgatcgcg
      421 ccgcgccgcc ggtgccctcg ctgattgggg cgctgctgcg cgttccagcc caggcgattc
      481 agcgccggct gatcgcgggc ctcaatgcct ccggcttctc cgagctgcgc cttccgcaca
      541 tggccgtgct gcagtacccg gggcccgacg gttgccggcc actcgaactc gcgttccgcg
      601 ccgggatcag caagcaggcg atgaatcagc tcctgaagag cctcgagcag cacggctacg
      661 tcacccgcga agacgcgaac gacgacctcc gctcgcgtcg cgtgcagctc acgaagcgcg
      721 gccgggccgt gtcgaagcgg atgatcgaac tgctggaggt gatcgagact gaatggcgcg
      781 gtgcgctcgg cgcgtatcgt ttcgatgagc tgtaagcgct cctcggcgaa gtgtggcggc
      841 tcgacctcag ccgcgcgacc gggcttgctg ctcagccgaa ctcgcgtcgt cgccctgcga
      901 agcgcaccgc gcagccgggt tcgcatcgtc gcgcggcgaa gcgtgccgcg cggggcgccg
      961 cacgactctg accttcccgc tcgacccgcc accgcagaag aagcgatcgc caccgtccga
     1021 ttcgagcccg gacacggcga cggccgcagg catttccagt cgttcctgca ccttgccggt
     1081 tcgggcatcg atcacggcga cgattctcga gatcgtggtt cagttggccg cgacaacggg
     1141 gaccgacgcg cgcacgtaca tgcgcgaacg cccagggatg agcgcgttcg tgcgcgagga
     1201 cggcgtcgtc tatcacgcgt gcaatttgcg cggtcaccga tcggatctac gcggctcgtc
     1261 gcagacatcg cgaccgcatt gcggcacgga gtacgactcg ggggtcactg cgtccgcagt
     1321 tgcgccgggc tgacgacgat cctcctcgtc accggattca tggaccttcg tgtgatggcg
     1381 ctcgtcacga tcgccatcac ggccgagcga ctgttgcccc cgcgttggcg cgtcgctcag
     1441 gcgatcggtt gcgttgccgt cgtcgtgggg atcgcgatga tcgggcgtgc ggttggctcg
     1501 ggctgagcgt tgatctggcg ccgagtcggg acgggtcagt gcggctgaat caccagagga
     1561 acgccggtga gaccgagatg aaagtccgtc gaccagcaag tgtgaagctt gcggacatgc
     1621 atcaggtgca gtccgacggc gtcggtgagt gtcagatcct ggtcatgaaa ccggcgcagc
     1681 aactgtgatg ccgcagcctg ctcgattttg ccgaccgacg cgatctcgag cggcgacatc
     1741 gcgtcgatca tcgcaagaaa ctgcaacgcg cgaccccggt cgtagcggcg gaggaaccat
     1801 gcatggccct ctgcgacgac gagcgacgat gtgatcagtg caggcggagt cgcgaacagc
     1861 cgaagaaacg tcccatgcca cgtgtcggaa cggtccgcga acgcgatgaa tgccgatgtg
     1921 tcgacgtaga cgtcaaccct tgcggccata cacgatgtcg tcgatatgct ggctcgattt
     1981 cggatcaccc gattcgacga agccggcata gcgcatccac ggcctggtcg catcgagcgg
     2041 aaggatgctc cgcaacgagc gacgcaggag ctccgcgagc gaaattccaa gccgcgcggc
     2101 ttcgcgtcgg gccgcgtcca gttcttcttc cgtgaggctg a
//
LOCUS       DASUCJ010000877         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_345644, whole genome shotgun sequence.
ACCESSION   DASUCJ010000877 DASUCJ010000000
VERSION     DASUCJ010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345644"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFU90_10480"
     CDS             <1..1213
                     /locus_tag="VFU90_10480"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480250.1"
                     /translation="GFPAVSLQPNAGSQGEYAGLLVIRRYHEARGEGHRDVCLIPQSA
                     HGTNPASAVMAGMSVVVVRTDDRGNIDLSDLREKAAANASRLAALMVTYPSTHGVFEE
                     GIRDLCQIVHANGGQVYMDGANMNAMVGLCRPADIGADVCHLNLHKTFCIPHGGGGPG
                     MGPIACAAHLAPFLPGHPLVRVGGTEAIGPVSAAPWGSASILPISYMYIELMGGEGLT
                     RASKVAILNANYMAKRLDAHYPVLYRGAHGTVAHECIIDPRAFKQSAGVDVEDIAKRL
                     MDYGFHAPTVSFPVAGTLMIEPTESESKEELDRFCDALISIREEVREIEDGRADRLDN
                     PLKNAPHTLERVLSSEWSRPYTREQAAFPAPWTREWKFWPSVGRVESAFGDRNLVCAC
                     PPIEEYASETA"
     gene            complement(1281..1670)
                     /gene="gcvH"
                     /locus_tag="VFU90_10485"
     CDS             complement(1281..1670)
                     /gene="gcvH"
                     /locus_tag="VFU90_10485"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5480251.1"
                     /translation="MSNIPNDLLYTEEHEFVKKLADSDVVQIGITDYAQGELGDVVYV
                     ELPKVGESFGRMDVFGTIEAVKAVSELFSPLAGTVEAVNARLDKEPALVNTDPYGEGW
                     MIRLRVKDAGEQDQLLGPEDYKKHIGE"
     gene            complement(1996..2619)
                     /gene="coaE"
                     /locus_tag="VFU90_10490"
     CDS             complement(1996..2619)
                     /gene="coaE"
                     /locus_tag="VFU90_10490"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5480252.1"
                     /translation="MMLIGLTGNIASGKSSVAKLFAARGATVIDADVLARRAVEPGTS
                     ALRSIVARWGTGVLSPDGSLDRAALRHVVFDRPDELEALNAIVHPEVGRLRDQEIAAA
                     AARGDRVVVCDIPLLFERRLVESFSRIVLVDAPRPIRLERLMRDRALSHEEAVQMIAA
                     QMPAELKRARADFVIENAGTREQLAERVSEVWDEIARDANATQPASA"
     gene            complement(2616..3656)
                     /locus_tag="VFU90_10495"
     CDS             complement(2616..3656)
                     /locus_tag="VFU90_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480253.1"
                     /translation="MKDAPVGPHAASVALSVQFQNGTPGEAIRVSIASFVPDSEQALD
                     SLFNGTFPVTSATQNLTVQFDLSSCINRFPPDNLGPFCEIEVSLELLNNGVVQDQQFL
                     GTMVVRPGQVVAAQPVVLTAGNNSPVITTLDTARVVEPSLIRYHLTGSDRDGDLTSLE
                     ATDFVNGNPDIESPYTFFPPLATVDGIFYAFQTTFAGANEIAVSLSDSKFNSSALDSV
                     PALPPIGDVFLDSLSVDTTADSILVNVNPQVDSLELVFLDETSSGETLAFTCGSAGPP
                     GLTTAHFACKRPAAFTSTMVVAVPVDFVGNPGNGVRCSVPTASCVPLPAADRRTSAPH
                     IQATRSPPRVHR"
     gene            complement(3826..4449)
                     /locus_tag="VFU90_10500"
     CDS             complement(3826..4449)
                     /locus_tag="VFU90_10500"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5480254.1"
                     /translation="MSDLGSLPGQMRSGWIEVIAGVMFSGKSEELIRRVRRAIIARKK
                     VQVFKSHLDDRYSGALTVVSHDGRSVEAEPVDASLQIAQRISPLVDVVAIDEAQFLDA
                     GVVELATALATRGIRVIVAGTETDFRGEPFGAMPQLMAVAEVVDKLHAICVICGGAAS
                     RNQRLLGGKPARYDSPTIMVGGHESYEARCRHCHQVPRRDDEQVPLL"
BASE COUNT          820 a         1487 c         1491 g          796 t
ORIGIN      
        1 cggtttcccg gcggtgtcgc tgcagcccaa cgcgggctcg cagggcgagt acgcggggct
       61 gctcgtgatc cggcggtacc acgaagcgcg cggcgagggc catcgcgacg tgtgtctcat
      121 tccgcagtcg gcgcacggca ccaatccggc gagcgccgtc atggcgggca tgtccgtggt
      181 cgtcgtgcgg accgacgacc gcggcaacat cgatctgagc gacctgcgcg agaaggcggc
      241 ggccaatgcg tcgcgcctgg ccgcgctcat ggtgacctac ccgtccaccc acggcgtgtt
      301 cgaggaaggc atccgcgatt tgtgtcagat cgtgcacgcc aacggcgggc aagtctacat
      361 ggacggcgcg aacatgaacg ccatggtggg gctgtgccgg cccgccgaca ttggcgccga
      421 cgtgtgccac ctgaacctgc acaagacgtt ctgcatcccg cacggcggcg gcggtccggg
      481 catggggccg atcgcgtgcg ccgcgcacct cgcgccgttc ctgccgggac atccgctggt
      541 gcgcgtgggc ggaacggaag cgatcggacc cgtgtccgcc gcgccgtggg gcagcgcgag
      601 catcctgccc atctcgtaca tgtacatcga gctcatgggc ggtgaaggcc taacgcgcgc
      661 ctccaaggtc gcgatcttga acgcgaacta catggccaag cgtctcgatg cgcactaccc
      721 cgtgttgtat cgcggggcgc acggcaccgt ggcgcacgag tgcatcatcg atccgcgagc
      781 attcaagcag agcgcgggcg tggatgtaga agacatcgcc aagcgcctca tggattacgg
      841 cttccatgca ccgacggtct cgttccccgt cgcgggcaca ctcatgatcg agcccaccga
      901 aagcgagtcc aaggaggaac tggatcggtt ctgcgacgcg ctcatctcca tccgcgagga
      961 ggtccgcgag atcgaggacg gacgcgcgga ccggttggac aacccgctca agaatgcgcc
     1021 ccacacgctc gagcgcgtgt tgtcgtcgga gtggtcgcgg ccctacacgc gcgaacaggc
     1081 agcgttcccg gcgccgtgga cccgcgagtg gaagttctgg ccgtccgttg ggcgcgtgga
     1141 aagcgcgttc ggggacagaa atctcgtgtg cgcgtgtccg ccaatcgagg agtacgcctc
     1201 ggaaaccgcc taacgatcga atgcggagaa cgccacgggc ccgcggccga gccgcgggcc
     1261 cgtggacatc gattccctgc ttattcgccg atgtgcttct tgtagtcctc gggaccgagc
     1321 agctggtcct gctcgcccgc atccttcacg cgcagccgaa tcatccagcc ttcaccgtag
     1381 ggatcggtgt tcaccagcgc cggttccttg tcgagccgcg cgttcactgc ttcgaccgtg
     1441 cccgcaagcg gtgagaacag ttccgaaacg gctttcaccg cttcgatcgt gccgaagaca
     1501 tccatgcggc cgaacgattc gccgactttg ggcagctcga cgtataccac gtcgcccaac
     1561 tcaccctgcg cgtagtcggt gattccgatc tgcacgacat cggagtcggc gagcttcttc
     1621 acgaattcgt gttcttcggt gtaaagcaga tcgtttggaa tgttcgacac aaaggctctg
     1681 cctaaaggtg aagtcgcgcg aagtctaccg gcgaggccgg gaggcgtcaa gcaaacgcgg
     1741 gagcgggtag aagtaccgtt tggtcgggta ccgcgctgag actcatcggt cgcgcccgag
     1801 tggcgtgagg catcctgcga ctcgctcctt gggggcttcc tcgctcttga tgatacggat
     1861 tgaacggatg acagcacgga gaacggccgc tctccgtgct gtcatccgct caatccgtgt
     1921 ctccagagcg tggaagccca ccacctcgca tcgctccggc atcggcgccc cgaactgaac
     1981 cggaaccggg gagcgtcagg ccgacgccgg ctgcgttgca ttcgcgtcgc gggcgatctc
     2041 atcccacact tcgctcacgc gttcggcgag ctgctcgcgc gtcccggcat tttcgatcac
     2101 gaagtcggcg cgcgcgcgct tgagctcggc cggcatctgc gcagcgatca tctgcaccgc
     2161 ttcttcgtgc gacaacgcac gatcgcgcat gagccgctcc agtcgaatgg gacgaggtgc
     2221 gtcgaccagg acgatgcgcg agaacgattc cacgaggcgg cgttcgaaca gcagcggaat
     2281 gtcgcagacg acgacgcggt cgccgcgcgc cgcggccgcg gcgatttcct gatcgcgcaa
     2341 tcgccccact tccggatgca cgatcgcatt gagcgcttcg agctcgtcgg gccggtcgaa
     2401 taccacgtga cgcagcgccg cccgatcgag cgagccgtcg ggcgacagca cgcccgttcc
     2461 ccagcgcgca acgatcgagc gcagtgcgga cgtgcccggt tcaactgcac gacgcgcgag
     2521 aacatccgca tcgatcacgg ttgcaccacg cgcggcgaac agtttcgcga ccgacgattt
     2581 gccgctcgcg atgttgccgg tgagaccgat gagcatcatc gatgcaccct cggaggcgat
     2641 ctcgtcgcct ggatgtgtgg cgccgaagtg cgccgatcag ctgccggaag cggaacacaa
     2701 gatgccgtcg gcacggaaca ccgaacaccg ttccccggat tgccgacgaa gtccaccggc
     2761 accgcgacaa ccattgtgct agtaaacgcc gccggccgct tgcacgcgaa gtgcgccgtg
     2821 gtcaaacccg gcggtccggc gctgccgcac gtgaatgcca acgtctcccc ggacgatgtc
     2881 tcgtcgagga agacgagctc gagactatcc acctgcggat tcacgttgac cagaatcgaa
     2941 tcggcggtgg tgtcgacact caaactgtcg aggaacacgt cgccgatcgg gggcaacgcc
     3001 ggcaccgagt ccagtgcact cgagttgaac ttcgagtcgg acaaactcac ggcgatctcg
     3061 ttcgcgccgg caaacgtcgt ctgaaacgca tagaagattc cgtcgaccgt cgccagcggc
     3121 gggaaaaacg tgtacggcga ctcgatgtcc ggattgccgt tcacgaaatc cgttgcctcg
     3181 agcgaggtca gatcaccgtc gcggtcgctc ccggtcagat ggtagcgaat caggctcggc
     3241 tcgacgacgc gtgcggtgtc gagggtcgtg atcactggtg aattattacc cgcggtgagg
     3301 acgactggtt gtgcagcgac gacctgaccg ggccgaacta ccatcgtgcc aaggaactgc
     3361 tggtcctgca ccacgccatt gttcagcaac tcgaggctga cttcgatctc gcagaacggt
     3421 cccagattat cgggtgggaa tcgattgatg cacgacgaca gatcgaactg aaccgtgaga
     3481 ttctgcgttg ccgacgtgac ggggaacgtg ccgttgaaca gcgagtcgag cgcttgctcc
     3541 gagtctggga cgaaggatgc gatggacaca cggatcgctt cgccgggcgt gccgttctgg
     3601 aattggacgc tgagcgcgac gctcgcggca tgcggcccca cgggcgcgtc tttcatgcat
     3661 gcggacgcaa gcagcgcaac ggcggccaac ccggatcggg cgattcggtt catgtcgagc
     3721 gtgtgttggg cctaggtcga gacgagcggc caaggtaggg cacgccgcgt ctcgctgtca
     3781 accggccgcg acgagaacgt tatgagtgtt ctaacgttcg cgcagctaca gcagcggcac
     3841 ctgttcgtcg tcgcgacgcg gaacctgatg acagtgtctg cagcgcgcct cgtacgactc
     3901 gtgtccgccg accatgatgg tgggcgaatc gtagcgggcc ggcttgccgc ccaggagtcg
     3961 ttgatttcga cttgctgctc cgccgcagat gacgcagatc gcgtgtaatt tgtcgacgac
     4021 ctcggcaact gccatgagct gcggcatcgc gccgaatggt tcgccgcgga aatccgtctc
     4081 ggtgccggcg acgatgacgc gaataccgcg cgtggccagc gctgttgcga gctcgacgac
     4141 acccgcatcg aggaattgcg cttcgtcgat cgcaacgaca tcgacgagcg ggctgatgcg
     4201 ctgtgcgatc tgcagggatg catcgacggg ctcggcctcg acgctccggc cgtcgtgact
     4261 caccaccgtg agcgccccgc tgtagcgatc atccaaatgt gacttgaaca cctgcacttt
     4321 cttcctggcg atgatcgcgc gacgcacgcg tcgaatgagc tcttcgcttt tgccgctgaa
     4381 catcacgccg gcgatgactt cgatccatcc ggagcgcatc tgtccgggga gagagcccag
     4441 gtcgctcact cgtcgcgccg tcgcgagggt ccgcgatcgc gcgtgccggg gcgtcgcggg
     4501 aaactgccgc ggggacgccc aacggagccg ccggctgcgc ctcgtgccgg gcgttcacgg
     4561 cgttcccccc ggtcttcccg gtcgcgccgt tcgc
//
LOCUS       DASUCJ010000878         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1461268, whole genome shotgun sequence.
ACCESSION   DASUCJ010000878 DASUCJ010000000
VERSION     DASUCJ010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461268"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFU90_10505"
     CDS             complement(<1..253)
                     /locus_tag="VFU90_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU5480255.1"
                     /translation="MFSKIGSFFSIRDDDEPYEEEFAPHSRVVPLSTAGRRGGTEVSV
                     YSPRSYQDVVEIADSLRNRQVVIVNLQNADRALLQRVVDI"
     gene            complement(256..1002)
                     /locus_tag="VFU90_10510"
     CDS             complement(256..1002)
                     /locus_tag="VFU90_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5480256.1"
                     /translation="MRDASGAAHRRFTGRFGAGIGGLAGLDAAGAPAERYRAFCRALD
                     AELAAGGRQPGAVTIVGISKKQPPAAVAEAIAAGLKDVGENYLQEARAKFGLLPPVRK
                     HFVGHLQTNKAKAVAELFDVVQSVDRLEAATALARAAQALGKRLPVLLQLNVSPYERF
                     GCDPHDANRLAEAIRALPGLRLEGVMAVGPLGDRGEPARAFELAAKTFARVGGSTLSI
                     GMSGDWREAVRAGSTMVRIGTALFGGRENR"
     gene            complement(933..>1353)
                     /locus_tag="VFU90_10515"
     CDS             complement(933..>1353)
                     /locus_tag="VFU90_10515"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5480257.1"
                     /translation="VRIVNFINDELWGDVCAPDRPWCMVPKDPSVWGYPNYLHPAQLY
                     EAVLDVLTLPVLLILYRTKPKDGVVGWTWFTLYGITRSVAEIWRHADFTWTGLTGGQL
                     YALPMIVIGIAGIAYCATRPGPRTDASPAGLEQASAG"
BASE COUNT          228 a          471 c          437 g          217 t
ORIGIN      
        1 tgatatcgac cacgcgctgc agcagcgcgc ggtcggcgtt ttggagattg acgatcacca
       61 cttgccggtt acgcagggaa tcggcgatct cgacgacgtc ttgatagctg cgcggcgaat
      121 agacgctcac ttccgtgccc ccgcggcgac cggccgtcga cagcggcacc actcgcgaat
      181 gcggtgcgaa ctcttcttcg tacggttcgt cgtcgtcgcg aatcgagaag aacgacccga
      241 tcttgctgaa catgctcaac ggttttccct cccgccaaag agcgccgtcc cgatccgaac
      301 catcgtcgat ccggcccgga cggcctcgcg ccagtctccc gacatgccga tggagagcgt
      361 acttccaccc acacgggcaa acgtcttggc ggccagctcg aatgcgcgcg ccggttcgcc
      421 gcgatcgccg agcgggccga ccgccatcac gccctccaga cgcaagcccg gcagcgcgcg
      481 aatcgcctcg gccagccggt tcgcatcgtg cggatcgcag ccgaaacgct cgtaggggga
      541 gacgttcagc tgcagcagca cgggcaagcg tttgcccaat gcctgcgccg cgcgcgcgag
      601 cgccgtcgcc gcttcgagcc gatcgacgct ctgaaccacg tcgaacagct cggccaccgc
      661 cttggccttg ttggtctgca agtggccgac gaaatgcttg cgtaccggcg ggagcaaccc
      721 gaactttgcc cgcgcttcct gcaggtaatt ctcgccgacg tccttcaggc cggccgcaat
      781 cgcctcggcc accgcggcgg gcggctgctt tttcgatatt ccgacgatcg tcacggcgcc
      841 cggttgccgt ccgccggcgg cgagctcggc gtcgagcgcg cggcagaacg cgcgatagcg
      901 ctccgccggg gcgccggcgg cgtcgaggcc tgctaacccg ccgatgcctg ctccaaaccg
      961 gccggtgaag cgtcggtgcg cggccccgga cgcgtcgcgc aatacgcgat gcccgcgatc
     1021 ccgatgacga tcatcggcaa ggcgtaaagt tgcccgcccg tcagccccgt ccaggtgaag
     1081 tccgcatggc gccagatttc ggcgacgctg cgggtaatgc cgtacagcgt gaaccacgtc
     1141 cagccgacga ctccgtcctt gggcttggtg cggtagagga tcagcagcac gggcagcgtg
     1201 aggacgtcga gaaccgcttc gtagagctgc gccgggtgaa gatagttggg gtagccccag
     1261 accgaggggt ccttggggac catgcaccac gggcggtcgg gagcgcagac gtcgccccac
     1321 agctcgtcgt tgatgaagtt cacgatccgc acc
//
LOCUS       DASUCJ010000879         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_895883, whole genome shotgun sequence.
ACCESSION   DASUCJ010000879 DASUCJ010000000
VERSION     DASUCJ010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895883"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VFU90_10520"
     CDS             complement(<1..1343)
                     /locus_tag="VFU90_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5480258.1"
                     /translation="MRASAGFAAFVVAAGERLLGRVLLQAGKIAVLAGILCAAIYRTG
                     AAETAGIVSGTVTDDRTHAPVPGTAVTAKSPSATYHDVTDARGHYVFLSVLPNNYSLS
                     FAHAGYAPYSTVVVVLNGSQQTVNAALSTTLKTIATTRARSAGSAFTHGMTIDTYTVT
                     GAQIETVQGKAFNTDENQLLRSIPSVTIDKSGTVSIRGGFAFEAAYEFEGIDYTAPSA
                     NLQNTLQNIGNFNLLNGVGSVQLIPGAGDATHGDTGTGLVLFAAKHGTYPQFVHVDAE
                     AQMFPYLHQLGLEWGWADPKQRFSNYAGFIGIRQAYQYGVPGTAANTLGTLGTNAATL
                     GSLIDPNLVYYAPSFLSSNDFVDNAIFRFGKNQSQRLQFFVQDQQITQTLDYGGFAFL
                     PYISGGTTAGKCASYPVIGPNGPVNTPQQNYTCDALIPLFPGQPNTYAFVSQPDTL"
BASE COUNT          247 a          399 c          483 g          263 t
ORIGIN      
        1 agcgtgtcgg gctgcgaaac gaaggcgtag gtattcggct gaccggggaa caacgggatc
       61 aacgcgtcgc aggtatagtt ctgttgcggc gtgttgaccg ggccgttcgg gccgatgacc
      121 ggataggacg cgcatttgcc ggcggtcgtc ccaccggaga tgtagggcag gaaggcaaag
      181 ccgccgtagt cgagcgtttg cgtgatctgc tgatcttgaa cgaagaactg cagacgctgg
      241 ctctggttct ttccaaaccg gaagatcgcg ttgtcgacga agtcgttgga cgaaaggaac
      301 gacggcgcgt agtagaccaa gttcggatcg atcaagctgc ccaacgtggc ggcgttggtg
      361 ccgagcgtcc cgagcgtgtt ggccgcggtt ccgggaactc cgtactgata cgcttgccgg
      421 atgccgatga atcccgcgta gttggaaaag cgctgtttgg ggtccgccca tccccactcc
      481 aggccgagct gatgcagata ggggaacatt tgggcttcgg cgtcgacgtg gacgaactgc
      541 gggtacgtgc cgtgcttggc ggcgaacagc acgagccccg tgccggtgtc gccgtgggtg
      601 gcgtcgccgg cgcccggaat caactgcacg ctgcccacgc cgttgagcag attgaagttg
      661 ccgatgttct gcagcgtatt ctgcaggttg gccgacggtg cggtgtagtc gatcccttcg
      721 aactcgtagg cggcctcgaa agcaaagccg ccgcgaatcg atacggtgcc gctcttgtcg
      781 atggtgacgc tcgggatgct gcgcagcagc tggttctcgt cggtgttgaa ggccttgcct
      841 tgtaccgttt cgatctgggc gccggtcacg gtgtaggtgt cgatcgtcat gccgtgcgta
      901 aaagcgctgc cggcggagcg cgcccgcgtc gtcgcgatcg tcttgagcgt cgtcgagagc
      961 gccgcgttga cggtttgctg cgagccgttg agcacgacga cgacggtcga gtacggtgcg
     1021 tacccggcgt gcgcgaacga tagcgaatag ttattgggga gcacgctcag gaagacatag
     1081 tggccgcgcg cgtcggtgac gtcgtggtag gtagcgctgg gcgacttcgc ggtaacggcg
     1141 gtgccgggaa ccggcgcgtg cgtgcggtcg tcggtaaccg ttccggagac gatgcccgcc
     1201 gtttcggccg cgccggtacg ataaatggcc gcgcacagga tcccggcgag gacggcgatc
     1261 ttccccgctt gcagcagaac tctgcccaac aacctctccc cagcagcaac cacgaacgcc
     1321 gcaaatcccg ccgacgctct catcacgatg ctaggagacg gcattgcggt ctgcatcccg
     1381 gaagacgcaa gc
//
LOCUS       DASUCJ010000880         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1125003, whole genome shotgun sequence.
ACCESSION   DASUCJ010000880 DASUCJ010000000
VERSION     DASUCJ010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125003"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..597)
                     /locus_tag="VFU90_10525"
     CDS             complement(214..597)
                     /locus_tag="VFU90_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5480259.1"
                     /translation="MEARQLFVDDDSPVPVYAQLSEQVLGAVARGDLHAGERLPSVRD
                     VAAALGVNANTVNHAYAQLEGIGVVETRRGRGTFVARFRARSAAPAASRLVELAEPFV
                     ARAQALGFSAAQIVNAVTGIVRRAR"
     gene            complement(679..1194)
                     /locus_tag="VFU90_10530"
     CDS             complement(679..1194)
                     /locus_tag="VFU90_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5480260.1"
                     /translation="MSGRPIVETERLIVRAWEDGDEEALIAIFADPGTVRYLGKGYQH
                     GLRADQTRAMVERMRERFARTGLGVWPVVLKETGAVIGECGLAPVADSDDVEIAYVLS
                     PAARGKGYASEAAAAVLAYAFQTLKLARVVAFVHRGNAASIAVINRLGMRYDRVVRAF
                     RADVMRYVALA"
     gene            complement(1191..>1578)
                     /locus_tag="VFU90_10535"
     CDS             complement(1191..>1578)
                     /locus_tag="VFU90_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5480261.1"
                     /translation="VVATDAPLDSRQLRAVALRAALGMGRVGLTSSVGSGDLIVAFST
                     THVFERTADFTVATPKGPIVEDDDTLDALYTATAEATQASIYDALFESKTTTGRRGAT
                     VYGLPVDRVLKMLQAAGAIPAAGAAP"
BASE COUNT          260 a          560 c          506 g          252 t
ORIGIN      
        1 gtcgcaggtt cgtcgccgga ccgcggcgcg gcacgacgtt gggcggcttg cgccgtttgc
       61 gtcgtttcat ggcaacctcc gggccgattc taccacgccc tgccgggaac gcctaacggc
      121 ggaccggcct tccggcacgg ccgcgccgga ggttcgcgcg gcggcgctcg taccaaacgg
      181 ccatcacgat aatccccgca accaagagga cgttcatcgc gctctcctta cgatgccggt
      241 aacggcattg acgatttgcg cagccgaaaa gcccagcgct tgagcgcggg cgacgaacgg
      301 ctccgcaagt tccaccaggc gcgacgctgc cggcgcggcg gatcgtgcgc gaaagcgcgc
      361 gacgaacgtt ccgcgaccgc ggcgggtctc cacgacgccg attccttcga gctgggcgta
      421 ggcgtgattg accgtgttgg cgttgacgcc gagtgccgcc gcgacgtcgc gcacgctggg
      481 caaccgctcc cccgcgtgca gatcgccgcg cgccaccgct ccgagcacct gctccgagag
      541 ctgggcgtac accggcaccg gtgagtcgtc gtccacgaat agctgacgtg cttccatcgc
      601 atctgtgtaa tagtgtccta ggactctagg acaccttaac tatacgcgaa tacggtgccg
      661 aggtcaagac tccagttctt acgcgagggc tacgtagcgc atgacgtctg cccggaacgc
      721 ccgcacgacc cggtcgtatc gcatcccgag gcgattgatc acggcaatcg aagccgcgtt
      781 gccgcggtgc acgaaggcca cgacccgcgc gagcttcaac gtttggaagg cgtacgcgag
      841 cacggcggcg gccgcttcgg aggcgtagcc tttcccccgc gcggcgggcg agagcacgta
      901 ggcgatctcg acgtcgtcgg agtccgcaac gggcgcgagc ccgcattccc caatcaccgc
      961 cccggtctct ttcaaaacga ccggccaaac gcccagaccg gtccgcgcga agcgctcgcg
     1021 catccgctcg accatcgcgc gcgtttgatc ggcgcgcaga ccgtgctggt agcccttgcc
     1081 gagatagcgc accgttccgg gatcggcgaa gatcgcaatc agcgcttcct cgtcgccgtc
     1141 ttcccaggcc ctcacgatca gccgctccgt ttcgacgatc gggcgcccgc tcacggcgcg
     1201 gcgccggccg ccggaatcgc gcccgccgct tgaagcatct ttaacacccg gtcgaccggg
     1261 agcccgtaca cggtagcgcc gcggcgtccg gtcgtcgtct tcgattcgaa cagcgcgtcg
     1321 tagatcgacg cctgcgtcgc ctcggcggtc gccgtgtaga gcgcgtcgag cgtgtcgtcg
     1381 tcttcgacga tcggaccctt cggagtcgcg accgtaaaat cggcggtgcg ctcgaatacg
     1441 tgcgtcgtag agaacgccac gatcaagtcg ccgctgccga ccgacgacgt cagtccgact
     1501 cgtcccattc ccagtgccgc gcgcaaggcc acagcgcgca gctgacggct atccaggggg
     1561 gcgtcggtcg cgacgacg
//
LOCUS       DASUCJ010000881         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1140620, whole genome shotgun sequence.
ACCESSION   DASUCJ010000881 DASUCJ010000000
VERSION     DASUCJ010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140620"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="glyA"
                     /locus_tag="VFU90_10540"
     CDS             <1..890
                     /gene="glyA"
                     /locus_tag="VFU90_10540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5480262.1"
                     /translation="GHLTHGWNVSATGQFWTPVRYEVDPQTHRIDYDKVRAIALAERP
                     KIIVAGATAYPREYDFKAFGEIAREAGAYFLADISHISGLIVAGVHPDPVPYADVVMT
                     TTHKTLRGPRGAMILCKEQHAEAIDKAVFPGLQGGPHENTIAGIGVALKEAATPDFGE
                     YGRQIVANAKALAAELGERGFDLITGGTDNHLILIDLTGKKVIGKKAAKALDRANIVG
                     TYNTIPFDPRKPFSPSGLRIGTPAVTTRGMKEDAMRRIGQWMDEVIDHVNDEDALDRI
                     AAEVRDFCRQYPAPGMHVA"
     gene            950..>1180
                     /locus_tag="VFU90_10545"
     CDS             950..>1180
                     /locus_tag="VFU90_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480263.1"
                     /translation="MALRGEGRLDDELACYDDVVAQFGYARPAEIVTQVALALLNKGV
                     ALGSHRMHERAIVAYNEVVRRFGERSESSLREH"
BASE COUNT          217 a          401 c          401 g          161 t
ORIGIN      
        1 gcggccacct gacccacggc tggaacgtca gcgcgaccgg ccagttctgg acgccggtcc
       61 gctacgaggt ggacccgcag acgcaccgca tcgactacga caaggtgcgc gcgatcgcgc
      121 tcgccgagcg cccgaagatc atcgttgcgg gcgcgacggc ctacccgcgc gagtacgact
      181 tcaaggcgtt cggcgagatc gcgcgcgagg ccggcgccta cttcctcgct gacatctcac
      241 acatcagcgg cctcatcgtc gccggcgtgc atcccgaccc ggtgccgtac gccgacgtcg
      301 taatgacgac gacccacaag acgctgcgcg ggccgcgcgg cgcgatgatc ctctgcaagg
      361 agcagcatgc cgaggcgatc gacaaagcgg tcttccccgg ccttcagggc gggccgcacg
      421 agaacacgat cgccggcatc ggcgtcgcgc tgaaggaggc cgcgacaccg gacttcggcg
      481 agtacggccg ccagatcgtc gccaacgcga aggcgctcgc ggccgagctc ggcgagcgcg
      541 gcttcgacct gatcaccggc ggcaccgaca accacctgat cctcatcgac ctcacgggga
      601 agaaggtgat cggcaagaag gcggcgaagg cgctcgaccg ggcgaacatc gtcggtacct
      661 acaacacgat ccccttcgac ccgcggaagc cgttcagccc gagtggcctg cggatcggca
      721 cgccggcggt gacgacgcgc gggatgaagg aagacgcgat gcgccgcatc ggccagtgga
      781 tggacgaagt catcgaccac gtcaacgacg aggacgccct cgaccgcatc gccgccgaag
      841 tgcgcgactt ctgccggcag taccccgctc cgggcatgca cgtggcgtag gcgtggggac
      901 cgagcacacg gccgatgccg acgttgcggc ggccaggatc cgtcgagggt tggcgttgcg
      961 cggcgaagga cggctggacg atgaactggc gtgctatgac gacgtggtag ctcagttcgg
     1021 ttacgcccgg cccgcagaga tcgtgactca ggttgcgctt gctttgctca acaagggcgt
     1081 cgccctcggc agtcaccgga tgcacgagcg cgccatcgtc gcgtacaacg aagtggtgcg
     1141 ccgtttcggc gagcgctccg aaagtagcct tcgcgagcat
//
LOCUS       DASUCJ010000882         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_452733, whole genome shotgun sequence.
ACCESSION   DASUCJ010000882 DASUCJ010000000
VERSION     DASUCJ010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452733"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..833
                     /locus_tag="VFU90_10550"
     CDS             300..833
                     /locus_tag="VFU90_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480264.1"
                     /translation="MKQLAVLLFIGMAAALPASAASATPCITLAHQSYCRVWSAKNAS
                     MSKTEFLRSGETVDRWQNMITVLRYNGLTMKEAIAHYMSIVRNYMGPDDHPVWIQPTH
                     GPHAEAAATRLVLVSPGVQGEYVVVYFFKDPGKPVYQVAFSQHLPLPTHDVPSMAQYG
                     KWLDDMQAMPAAAVAAK"
     gene            900..>1651
                     /locus_tag="VFU90_10555"
     CDS             900..>1651
                     /locus_tag="VFU90_10555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5480265.1"
                     /translation="MAPLSVRFRYLLAVAAIASFGAGAGPSVAAGTNAPAAVPAAIRA
                     VFAKPQYKDSTWALRVEDGSKVLIDYNSAKPLYIGSVRKVFSVGELMNAVGPTHTYDT
                     PIYRTGSVSGGVLHGNLVLVASGDLTMGGRRNPDGTIAVSDWDHNEADSLGNAILTAP
                     DPLAGYRQLAQAVRAAGIRRVAGDVAIDDRLFQPFFFRDQFNVRPIFVNDDLVDVTIA
                     PGGRPGSRANLTSRPQSAALAVKNQLVTGAPNS"
BASE COUNT          289 a          580 c          496 g          286 t
ORIGIN      
        1 gtgaaagcgc actcgcgtgc cggccggcac ttgcggcggc gcgacggccg gatcgctcgc
       61 cgcgatcgtc gccgctacta gtgcgaatat tgcaaaaaga cgcggcgcgg tataacgcgc
      121 caaatgccgt accggcagtc cgaagcccac acccatgccg cggtgtttat tcgcgctcgc
      181 agacgagccc ttgctcaccc gagcgaactc taggccattt gtcccaaagg ctggcttacg
      241 ctcgaactgc cgctgggcag ccaacggggc cgaaacggcc gagcgccaat acccgcccga
      301 tgaaacagct cgccgttttg ctcttcatcg gaatggccgc cgctctgccc gcgagcgccg
      361 catccgctac gccctgcatc acgctcgccc atcagtcgta ctgccgcgtt tggagcgcga
      421 aaaacgcgtc gatgagcaag acggagttct tgcgctccgg cgagacggtc gaccgctggc
      481 aaaacatgat caccgtcctg cgctataacg ggctcacgat gaaagaagcg atcgcgcact
      541 acatgtcgat cgtgcgcaac tacatggggc ccgacgacca tccggtgtgg atccagccca
      601 cccacggtcc gcacgcggag gcggcggcga cgcggctggt gctcgtgagt cccggcgtgc
      661 aaggcgaata cgtcgtggtc tacttcttca aagatcccgg gaagccggtc tatcaagtcg
      721 cgttttcaca gcacttgccg ttgcccacgc acgacgttcc gtcgatggcg cagtacggca
      781 agtggctcga cgacatgcag gccatgcccg ccgccgccgt ggctgcgaaa tagcgtaaca
      841 aggtgctccg caacgctgcg gcgcacgttg gaatgtcgct tttttcgcgg aggtttctcg
      901 tggcgccgtt gtccgttcgc tttcgctatc tgctcgccgt tgctgcaata gcgtcgttcg
      961 gcgccggcgc cggtccgagc gtcgccgcgg ggacgaatgc ccccgccgcc gttccagccg
     1021 ccattcgcgc ggtcttcgca aaaccgcagt acaaagactc cacctgggcg ctgcgcgtcg
     1081 aggacggttc caaggtgctg atcgactaca actccgccaa gccgctctac atcggctcgg
     1141 tacgcaaggt cttcagcgtc ggcgagttga tgaatgcggt cggccctacg cacacctacg
     1201 acacgccgat ctatcgcacc ggaagcgtct ccggcggcgt gttgcacggc aacctcgtgc
     1261 tggtcgcgtc cggcgacctg acgatgggcg gtcgccgcaa ccccgacggc accatcgcgg
     1321 tgagcgattg ggaccataac gaagccgaca gcctcggcaa cgccattctc acggcgcccg
     1381 atccgctggc cgggtaccgg cagctggccc aggcggttcg cgccgccggc atccgccgcg
     1441 tcgccggcga cgtcgcgatc gacgaccggc tcttccagcc gttctttttc cgcgaccagt
     1501 tcaacgttcg cccgatcttc gtcaacgacg atctcgtcga cgtcacgatc gcaccgggcg
     1561 gtcgaccggg atcgcgcgca aacctgacgt ctcgtccgca atccgccgcg ctcgccgtca
     1621 agaatcaact cgtcaccggc gcgcccaact c
//
LOCUS       DASUCJ010000883         1414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_804391, whole genome shotgun sequence.
ACCESSION   DASUCJ010000883 DASUCJ010000000
VERSION     DASUCJ010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804391"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFU90_10560"
     CDS             complement(<1..303)
                     /locus_tag="VFU90_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU5480266.1"
                     /translation="MKKKLVAVAVAGILGMPLAAQAQTANVVLYGRLNLTTEFVKGQQ
                     ADGSNPTAVRLSSNSSRFGMRGVESLGGGLNAIFQIESSINGDTNGGNLGGRETFVG"
     gene            686..>1414
                     /locus_tag="VFU90_10565"
     CDS             686..>1414
                     /locus_tag="VFU90_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480267.1"
                     /translation="MADSGMVMATPVWEASALAALLSAAALAALVGLRRALPQDVPNA
                     RSLHARRVPRAGGFAIWAGFIPAALLFPPSFPGGTSAWLVPWAVLAGVSAWDDRRVVP
                     IVPRLAVHAVAALWVGWKLVGDDAGVSSVLPVWLVVIGIGVIVAWASNLYNFMDGNDG
                     LAATMGVIGFASMGFATRDSATAPALFALAAAILPFLAVNRPPATMFLGDAGAVPLGF
                     LAAAFGIEGVVASRWPPWFPLLVFL"
BASE COUNT          191 a          473 c          472 g          278 t
ORIGIN      
        1 gccgacgaag gtttcacggc cgccgaggtt gccaccgttc gtgtcgccgt tgatcgacga
       61 ttcgatctgg aagatcgcgt tcagtccgcc accgagcgac tcgacgccgc gcatgccgaa
      121 gcgcgacgag ttgctggaca gacgcaccgc ggtcgggttg gaaccatcgg cctgctggcc
      181 cttcacgaac tcggtggtca ggttcaggcg gccgtaaagc acgacgttcg cggtctgcgc
      241 ctgcgcagcc agcggcattc cgagaatgcc cgcgactgcg acagcgacga gcttcttctt
      301 cattgagact ctcctatatc ggaaaaattt ggcaaggaaa cagccgactg cgcctgccat
      361 cgaaaccttc tctactctgg cgagaagttg agtccattgt gccaaacggc cgaaagcatt
      421 ggcaaggcaa ctcgcgggcg ggctcgcacc ctctgatccg ctgttgtgcc gaagcaacac
      481 ggggccgccc ggccgcaatc cgggcctgtt cgagcttcgc ccgttaaatc cttgatccgc
      541 atgggaaagc gactcgaatc caccggtgcg ccgcccgtgc gagcccgaat gccgtgattt
      601 tcaccagaaa agtcctgccg caccgcctct cgcgcgccac tttcctgccg gtatttcccc
      661 cgattggggc aaaatccgcg cctcgatggc cgactccggg atggtgatgg cgactcccgt
      721 ttgggaggcg agcgcgctcg cggcgcttct atcggcggct gcgctcgccg cgctcgtcgg
      781 attgcgtcgc gcgcttcccc aggacgtccc gaatgcgcgc tcgctgcacg cgcgacgggt
      841 gccgcgcgca gggggattcg ccatctgggc gggattcatt ccggcggcgc tcctgttccc
      901 gccttcgttc ccgggcggga cttccgcgtg gctcgtgccg tgggcggtgc tggcgggggt
      961 gtcggcgtgg gacgaccggc gtgtcgtgcc catcgttccc aggcttgccg tgcacgcggt
     1021 cgccgcgttg tgggtcggct ggaagctcgt cggcgatgac gccggggtgt cgagcgtgtt
     1081 gcccgtctgg ctggtcgtca tcggcatcgg cgtgatcgtc gcgtgggcat cgaacctcta
     1141 caacttcatg gatggcaacg atgggctcgc ggcgacgatg ggggtgatcg gcttcgcttc
     1201 gatgggcttc gcgacgcgcg acagcgccac cgctccggcg ctcttcgcgc tcgccgcggc
     1261 gattctgccg ttcctcgccg tcaatcggcc cccggcgacg atgttcctcg gcgacgcggg
     1321 tgcggtgccg ctcgggtttc tcgccgcggc cttcgggatc gagggcgtcg tcgcgtcgcg
     1381 ctggccaccg tggtttcccc tcctcgtgtt cctg
//
LOCUS       DASUCJ010000884         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_177329, whole genome shotgun sequence.
ACCESSION   DASUCJ010000884 DASUCJ010000000
VERSION     DASUCJ010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177329"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..>1256
                     /locus_tag="VFU90_10570"
     CDS             181..>1256
                     /locus_tag="VFU90_10570"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480268.1"
                     /translation="MKAPVDARLIRGTSLITALAALSLAAAAHAGAPHKFLDGKLSWR
                     SIGPNIGGRSVAVAGVPSERNLFYMGGVDGGVWKSTDYGVVWNNITDGKWPSASDSIG
                     AIAVAPSNPKVLYVGTGETDIRNDMITGDGIFKSVDAGKSWKYAGLRDTRTISAIVVD
                     PRDPNVVYTSSMGHVFAPNADRGVFKSTDGGKTWQKILFVDDKTGAIDLVMDPRDPKV
                     LYAAMWQAQRTPWSLDDGGPGSGLYKTTDGGAHWTNLSHNPGLPGGVLGRIGVDVAAS
                     NPSIVFAIVQAKGGGVFRSADAGATWHRVNDKWSLRQRAFYYMSIYVDPTNPKTVYVP
                     NVDGVWVSHDGGKGFSLLHMRHGD"
BASE COUNT          224 a          424 c          401 g          207 t
ORIGIN      
        1 aatcgccggg cgtcgtctcg gccccgccga gtctcgtctc accgcgtttt gcaggctccg
       61 agcccctctc gagcgcgcgc gccgttggtt gattgcggcg tgggattcag ttcgctaagc
      121 tcgatcgatt ccattttccc gcgttccctg gcaacaaggg cgcagaggag ttctcgcatc
      181 atgaaggcac ccgtcgacgc gcggctcatc cgcggcacct cgctcatcac ggctctggcg
      241 gcgctgtctc tcgccgcggc cgctcacgcc ggcgcgccac acaagttcct cgacggcaag
      301 ctctcctggc gcagcatcgg accgaacatc ggcggacgat cggtggcggt ggcgggcgtc
      361 ccgagcgagc ggaacctgtt ctacatgggc ggtgtcgacg gcggcgtctg gaagagcacg
      421 gattacgggg tcgtctggaa caacatcacc gacggcaaat ggccctcggc gagcgacagc
      481 atcggcgcga tcgcagtggc gccctcgaac ccgaaggtgc tctacgtcgg cacgggcgag
      541 accgacatcc gcaacgacat gatcacgggc gatggcatct tcaagtccgt ggacgccggc
      601 aaatcctgga aatatgccgg actgcgcgat acccgcacga tcagcgcgat cgtcgtcgac
      661 ccgcgcgatc cgaacgtggt ctatacctct tcgatggggc acgtgttcgc acccaacgcc
      721 gaccgcggcg tcttcaaatc cacagacggc ggcaaaacgt ggcaaaagat cctcttcgtc
      781 gatgacaaga cgggcgccat cgacctcgtg atggatccgc gcgaccccaa ggtgctctac
      841 gccgcgatgt ggcaggcgca gcgcaccccc tggtcactcg atgacggcgg tccgggaagc
      901 ggtctttaca agaccaccga cggcggcgcg cactggacca acctttcgca caatccgggc
      961 ttgccgggcg gcgtcctcgg ccgcatcggc gtcgacgtcg cggcgagcaa cccgagcatc
     1021 gtctttgcga tcgtgcaggc gaagggcggc ggcgtgttcc gctcggcgga cgccggcgcg
     1081 acctggcacc gcgtcaatga caagtggagc ctgcgccagc gcgcgttcta ctacatgagc
     1141 atctacgtcg atccgacgaa cccgaagacc gtatacgtgc cgaacgtcga tggcgtgtgg
     1201 gtgtcccatg acggcggcaa ggggttctcc ctcctgcaca tgcgccacgg agacaa
//
LOCUS       DASUCJ010000885         1413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1140645, whole genome shotgun sequence.
ACCESSION   DASUCJ010000885 DASUCJ010000000
VERSION     DASUCJ010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140645"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VFU90_10575"
     CDS             <1..1322
                     /locus_tag="VFU90_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480269.1"
                     /translation="AFALSACAGNNSVPGPQAGVVPAHTAQRGAASSPIEHVVIIVQE
                     NRSFDNLFATFPGANGATQGKMHNGNVIPLKEKGLPGIDVNHDSRAYNGDYDGGKMDG
                     FDLELFEGRAVGKYPYQYVNPADIAEYWTLAQQYALADAMFQTQGSGSFTAHQDLIAG
                     ATAIDSTDSLIDYPNESTNWGCGARTGTKTSLITTQGQYLRYQGPFPCLTYPTGTMRD
                     LLDAAGVSWKYYTPAYKNDTVGALWDAFAAIQAVRKGPEWNTNISKPETNVLTDAANG
                     TLPAVSWVIPDQIDSDHPHSPNGQDDGPSWVANVVNAIGGGSDWNSTAIVILWDDWGG
                     YYDHVPPPFFDNAGGLGFRVPMIVVSPYVKAGTIAHTQYEFGSILKFVEQTFNLGSLG
                     TTDVRATSIGNVFNFKMRPRRFHPIATLHTRAFFLHHPPDDEPVDTQ"
BASE COUNT          282 a          488 c          429 g          214 t
ORIGIN      
        1 tggcgtttgc tctgagcgcg tgcgccggca acaactcggt gcccggaccg caagccggcg
       61 tcgtgccggc gcacaccgcg cagcgcggcg ccgcgagcag cccgattgag cacgtcgtca
      121 tcatcgtcca ggaaaaccgc agcttcgata acctgttcgc gacgttcccc ggcgccaacg
      181 gcgcgaccca gggcaagatg cacaacggca acgtgatacc gctcaaggaa aaagggctgc
      241 cggggatcga cgtcaaccac gactcgcgcg cctacaacgg cgactacgac ggcggcaaga
      301 tggacgggtt cgatctcgag ttgttcgagg gccgggctgt cggcaaatac ccgtaccaat
      361 acgtgaatcc ggccgacatc gccgagtact ggacgctggc ccagcagtac gcgctggccg
      421 acgccatgtt ccaaacgcaa ggcagcggca gctttaccgc gcaccaagac ctcatcgcgg
      481 gcgccaccgc gatcgactcg accgacagtc tgatcgacta tcccaatgag agtaccaact
      541 ggggctgcgg cgcgcgcacc ggcacgaaga cctcgctgat taccacgcag gggcagtacc
      601 tgcgctatca agggccgttc ccgtgcctca cctatcccac cggcacgatg cgcgatctgc
      661 tcgacgccgc cggcgtcagc tggaaatatt acacgcccgc ctacaagaac gacacggtcg
      721 gtgcgttgtg ggacgcgttc gcggccatcc aggccgtgcg caaaggcccc gagtggaaca
      781 ccaacatctc caaacccgaa acgaacgtgc tcaccgacgc cgccaacggc acgctgccgg
      841 cggtgtcgtg ggtcattccc gaccagatcg actccgatca cccgcactcg cccaacggac
      901 aggacgacgg cccatcctgg gtggcgaacg tcgtcaacgc gatcggcggc gggagcgact
      961 ggaactcgac cgcgatcgtc atcctgtggg acgactgggg cgggtattac gaccacgtgc
     1021 cgccgccgtt tttcgacaat gccggcggtc tcggattccg cgtgccgatg atcgtcgttt
     1081 caccctacgt caaagccggc acgatcgcgc acacgcagta cgagttcggc agcatcctca
     1141 agttcgtcga gcagacattc aacctcggct cgttggggac gaccgacgtc cgggcgacct
     1201 cgatcggcaa cgtcttcaac ttcaagatgc ggccgcgccg cttccatccg atcgcaacgt
     1261 tgcacacgcg agccttcttc ctgcaccatc cgcccgacga cgagccggtc gacacgcagt
     1321 aacggcgtgg ccgagccatc gagctgtgtc atcctgagcg gaggcgcgta gcgccgcagt
     1381 cgaagggctg agcggagcgc cccagtgtta gcg
//
LOCUS       DASUCJ010000886         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_727641, whole genome shotgun sequence.
ACCESSION   DASUCJ010000886 DASUCJ010000000
VERSION     DASUCJ010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727641"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFU90_10580"
     CDS             <1..938
                     /locus_tag="VFU90_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEU5480270.1"
                     /translation="IRNLDNSLTSLQAAGRAFYFNSNASGAEIPVDRQHSCGGCHVLD
                     PNGNRGQTAHPGFFGTDGRLSFDNESQILKVPHLRNAYQKLGMYGASLDAVRALASII
                     PQLNPPVNAVRGFGYNHDGVLGTIEQFASQEAFVLNTSGTVPNPGGIPFFNNPANPFD
                     SSSGLSSQGAATRRSLAAFLLAYDSNLAPIVGQQLTLTRDNAAASADRLALLIAQATA
                     GACDLVVHGNVFGRDTGFVFQDGAFQSDGCSKKALSSADLVALASRDGKVALTFTAVP
                     PGSGWRLGIDRDSDGFGDSDEASAGTDPADPSSTP"
     gene            complement(941..>1222)
                     /locus_tag="VFU90_10585"
     CDS             complement(941..>1222)
                     /locus_tag="VFU90_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480271.1"
                     /translation="AIAQLTLNPLYFLSGALYPVNGLPGWLLVLTRVDPLSYAVDPMR
                     RAVLGRVGVPVAAMTWAGTPVPIAAELGVLAAIALAALFLATLRFRQAD"
BASE COUNT          217 a          432 c          396 g          177 t
ORIGIN      
        1 cgatacgcaa cctcgacaac tcgctcacgt cgctgcaagc ggcggggcgc gcgttctact
       61 tcaacagcaa cgcgagcggc gcggagatcc ccgtggaccg ccagcacagc tgcggcgggt
      121 gccacgtgct cgaccccaac ggcaatcgcg ggcagacggc gcacccgggg ttcttcggga
      181 ccgatgggcg gctctccttc gacaacgagt cgcaaatcct caaagtcccg cacctgcgca
      241 acgcctacca gaagctcggt atgtacggtg cgagcctcga tgcggtgcgc gccctcgcgt
      301 cgatcatccc gcagctgaat ccgccggtga acgcggtgcg cggcttcggg tacaaccatg
      361 acggcgtcct cggtacgatc gagcaattcg cctcgcaaga ggcattcgtc ctgaacacgt
      421 cgggtaccgt tccgaacccg ggcggcattc cgttcttcaa caacccggcc aatccgttcg
      481 attcgagcag cggtctgtcg tcacaaggcg cggcgacgcg ccggtcgctg gccgcgttcc
      541 tcctcgctta cgacagcaac ctggccccga tcgtcggcca acagctcacg cttacacgcg
      601 acaacgcggc cgcgagcgcc gaccgcctgg ccctgctcat cgcgcaagcg actgcgggcg
      661 cttgcgatct cgtggtccat gggaacgtct ttgggcgcga taccggcttc gtattccagg
      721 acggcgcatt tcagtccgac gggtgctcga agaaggcgct ctcctccgcg gatctcgtgg
      781 ctctcgcgag ccgcgacgga aaggtggcgc tgacgttcac cgcggttccg ccgggctccg
      841 gctggcgcct cggcatcgac cgagactcgg acggattcgg ggacagcgac gaggcttccg
      901 ccggcaccga cccggcggat ccgagcagca ctccgtaacg tcaatccgcc tggcgaaacc
      961 gcagcgtcgc caagaacagc gcggccagcg cgatcgccgc caagaccccc agctcggcgg
     1021 cgatcggaac gggagtgccg gcccacgtca tcgcggcgac cggcaccccg acgcgcccca
     1081 gaacggcgcg tcgcataggg tcgacggcgt agctcaaggg gtcgacccgg gtcaggacga
     1141 ggagccaacc gggcaggccg ttgaccggat agagcgcgcc cgaaagaaag tacagcgggt
     1201 tgagcgtcag ctgcgcgatc gc
//
LOCUS       DASUCJ010000887         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_116680, whole genome shotgun sequence.
ACCESSION   DASUCJ010000887 DASUCJ010000000
VERSION     DASUCJ010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116680"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFU90_10590"
     CDS             <1..826
                     /locus_tag="VFU90_10590"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEU5480272.1"
                     /translation="ALLRDPARHRIAASARVLDVDPPLLTKRGAAAARSRELADLSSP
                     NEDEAAMARLRQRGIVRSAELRNAGVRALPKPFTGDWIVDDQRAAELRGKLSDFVATH
                     ARMFPLDPGPTIDATRRALDLPDRALVLALIETSDDAFAVRDGRILERARGTALPPRV
                     AEAIGQIRSRLEGNPFAAPETTELSSLGLGPRELAAAERAGLLWRVSPSIVLRPDAPE
                     VAVRKLAALAQPFTVSQAREALDTTRRVAVPLLEFLDRNRFTRRLADDTRTVIEPS"
     gene            856..>1090
                     /locus_tag="VFU90_10595"
     CDS             856..>1090
                     /locus_tag="VFU90_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480273.1"
                     /translation="MARRTFLDWPVVRQVLDGDPSGRGSAVMSATTKQQQARTETADR
                     VVKSICPYCAVGCGQNVYVKDDKVVQIEGDPDSP"
BASE COUNT          199 a          379 c          359 g          153 t
ORIGIN      
        1 cgcgctgctg cgcgaccccg cgcgtcatcg catcgccgca agcgcgcgcg tcctcgacgt
       61 cgacccaccc cttttgacga aaaggggcgc tgcggcggcg cggagccgcg aactcgccga
      121 tctgtcgagc ccgaacgagg acgaggcagc gatggcccgg ttacggcaac gcggcatcgt
      181 gcgcagtgcc gaactccgca acgccggcgt gcgcgcgctg cccaagccgt tcaccggcga
      241 ctggatcgtc gacgatcagc gggccgccga actgcgcggc aagctaagcg atttcgtggc
      301 aactcacgcg cggatgttcc ccctcgaccc cggccccacg atcgacgcca cccgccgcgc
      361 gctcgacctc cctgaccgag cactggtgct cgccttgatc gagacatcgg acgacgcgtt
      421 cgccgttcgc gacggccgga tactcgaacg cgcgcgcggg accgcgttac caccacgggt
      481 cgctgaagcg atcggccaga tccgctcacg actggagggc aatccgttcg cagctcccga
      541 gaccaccgaa ctcagcagcc tcggactggg cccgcgcgaa ctcgcggccg cggaacgggc
      601 gggactgttg tggcgggtga gcccaagcat cgtgctacga cctgatgcgc cggaggtcgc
      661 cgtccgaaag cttgcagcgc tagcacagcc gttcaccgtc agccaggcgc gggaggcgct
      721 cgacacgacg cgccgagtgg ctgttccact cttggaattt ctggatcgaa ataggtttac
      781 ccgaaggctt gccgacgaca cacgtactgt catagaaccg agttgacccg gcggacgagg
      841 aagggagcca gcccgatggc gcgacgaacc ttcctcgact ggccggtcgt gcggcaagtg
      901 ctcgacggcg acccaagtgg ccgcgggtcg gccgtcatgt cggctacgac gaagcaacag
      961 caggcgcgca ccgagaccgc tgaccgcgtg gtgaagagca tttgcccgta ctgcgccgtc
     1021 ggttgcggcc aaaacgtcta tgtgaaagac gacaaggtcg tccagatcga gggcgatccc
     1081 gacagcccgg
//
LOCUS       DASUCJ010000888         5074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1522483, whole genome shotgun sequence.
ACCESSION   DASUCJ010000888 DASUCJ010000000
VERSION     DASUCJ010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522483"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..684
                     /locus_tag="VFU90_10600"
     CDS             100..684
                     /locus_tag="VFU90_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480274.1"
                     /translation="MDEGRFRFDLYVAVLALAISGVAAFASAYQTYVIRQQFSATVWP
                     YLTFTTRSSTDNYFELDLGNAGIGPALIRSASVTRDGKPIDVASNPTASPAISVAIAP
                     DQAEAKSDELRAKYHGKASTTVSSIVRGDVVPAGSTLQLLRVDGKFLTRRLLADARHL
                     DLQICYCSLLGNCWTKRLWDPSAEPHPVRSCPDA"
     gene            681..1190
                     /locus_tag="VFU90_10605"
     CDS             681..1190
                     /locus_tag="VFU90_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEU5480275.1"
                     /translation="MTGPVSRRRVRAAGAADLPAIRRIYNEGIADRQATLESEPKTAA
                     EIDAWFARHTGRCAVLVAEEDGGVAGWASLAPFSHRCAHAGIADVSIYVARERRGCGI
                     GTTLLTALEDAARAGGFHKLVLHALDRNAPAKHLYRKAGFSQVGVFAEHGILDGRYED
                     VVAMEKLLR"
     gene            complement(1151..2467)
                     /locus_tag="VFU90_10610"
     CDS             complement(1151..2467)
                     /locus_tag="VFU90_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5480276.1"
                     /translation="MSKKLPGPGTATTLRRLMPMPFLKFVPFLREMTARYGNLYAFSV
                     PWRRYVFINEPVLIKDVLVTQQQAFSKSLGTRMLRHLLGEGLLTSEDPLHRQMRRIVQ
                     PAFHRERIGRYAAAMEAAADDFANGLRADRPFDLHAAMTALTLRIASVTLFGSDASDA
                     AQRVSAALGLMMREFPYVLLPFGELRRRLPLPATRRFQAAKKSLDDIIYALIALRRET
                     PGDDALSMLLAAHDAESGYRPSDEQIRDEVMTLFMAGHETTANLLVWTWYLLAQHPDV
                     DAATAAAAIDGDGDFIDRVVLESLRLYPPAWVVGRESRRAVTLSDGTLVAPKTTVLIA
                     PLLLHRRPEFFRDPDRFDPDRWLGAEPAPFSYLPFGAGARRCIGNDFALREAAIVLRA
                     VTARFSFALEPGVDVRPAPLVTLRPDGPVPVRPSYRRSFSIATTSS"
     gene            complement(2464..2736)
                     /locus_tag="VFU90_10615"
     CDS             complement(2464..2736)
                     /locus_tag="VFU90_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEU5480277.1"
                     /translation="MPADRRSLQSHSLPDDGVVRIMRERRRASTVSVVTGLRAPELAE
                     IAKLLKRHCGTGGTAKNGVVELQGDHREKIAEWFAQRGRKVKLAGG"
     gene            2789..3337
                     /locus_tag="VFU90_10620"
     CDS             2789..3337
                     /locus_tag="VFU90_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5480278.1"
                     /translation="MQRRICVFCGSSSGNDASYRSAARDAARAIVRAGYGVVYGGGRI
                     GLMGELADAALAAGGEVVGVIPAALARVEIAHDGLTQLHVVDSMHERKALMARLSDAF
                     VALPGAFGTMDEFCEILSWRQLAIHDKPIGLLNHRGYFDRLLDLFDAMVREGFLLPAN
                     RKLVVDAAEIEPLLAAMNVNTK"
     gene            3423..3920
                     /locus_tag="VFU90_10625"
     CDS             3423..3920
                     /locus_tag="VFU90_10625"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU5480279.1"
                     /translation="MGFGSLSLDFLALPLEDAEALGAALVELQTLSAQPPVGPEELAD
                     ARRLQAALAACTLAAVRREPLPAHDVATLNSYAGDEPPALVLRTDGTLARAGTDPVRA
                     ALAAIARDAGETIACRAGELRRCEGANCGRLFADDSRGRRRRWCSMARCGNRVKAAAF
                     RRRPA"
     gene            complement(3886..>5074)
                     /locus_tag="VFU90_10630"
     CDS             complement(3886..>5074)
                     /locus_tag="VFU90_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5480280.1"
                     /translation="VVAIDVRRPDAARTVVPEAAAALQGASAVGKRLILSYLVDAHSA
                     VKVYDYAGKFVRDVALPGLGNAGGFAGFSNDRETFYAYSGWTTPPTLYRYDVVSGKST
                     VYRKPQIAFDPSGYDTKEVFYRSKDGTRVPMMISARKGIALDGSNPTILYGYGGFDIP
                     MTPYFSSFIATWLQMGGIYAVANIRGGSEYGEAWHRGGMLGNKQHVFDDFIAAAEYLI
                     AQKYTSTPKLAIKGESNGGLLVGAVETQRPDLFGAAVPGVGVLDMLRFDKFTIGNFWI
                     PEYGCSTCGAADFAWLYKYSPYANVKAGVAYPPTLIVTSDHDDRVFPAHSFKFAARMQ
                     AAQSGPAPVLLRVQLKAGHGESTTLAQSIDLTADVYAFLVKNLAMTLPAGFTPAGGET
                     PRP"
BASE COUNT          838 a         1709 c         1706 g          821 t
ORIGIN      
        1 cgctgggcgg tgcgcgagat ccaggaccag atgattcacg aggcggtccg caaggccggc
       61 gtgggggacg aacagctcgt cttagcgtaa gccgaccgca tggacgaagg ccgcttccgc
      121 ttcgatctct acgtcgccgt gctcgcgctg gccatcagcg gcgtggcggc gtttgcctcg
      181 gcgtatcaga cctacgtcat ccgccagcag ttcagcgcga ccgtttggcc gtaccttacg
      241 ttcaccacgc ggtcgtcgac cgacaactac ttcgagctcg acctcggcaa cgccggaatc
      301 ggaccggcgc tgatccgcag cgcgagcgtc acccgcgacg ggaaaccgat cgacgtcgct
      361 tcgaacccca cggccagccc ggcaatttcg gtcgcgatcg cgccggatca agccgaagcc
      421 aagagcgacg agctccgcgc gaaatatcac ggcaaggcga gcacgaccgt gagctcgatc
      481 gtgcgcggcg acgtcgttcc cgccggctcg accctgcagc tgctgcgcgt cgacgggaag
      541 ttcctcacgc gccgcctgct cgccgacgcc cggcacctcg atctgcagat ttgctactgc
      601 tcgctgttgg ggaattgctg gacgaaacgt ctgtgggatc cgtcggcgga accgcatccg
      661 gttcgttcct gtcccgacgc gtgaccggac ccgtgtcgcg ccggcgcgtc cgcgcggcgg
      721 gcgccgccga cctgcccgcg atccggcgca tctataacga aggtatcgcc gatcgccaag
      781 ctaccctcga gtccgagccg aaaacggcgg ctgaaatcga tgcgtggttc gcacggcata
      841 ccggacgctg tgccgtgctg gtcgccgagg aagacggcgg cgtcgccggc tgggcgtcgc
      901 tggccccatt ctcgcaccgc tgcgcgcacg ccgggatcgc cgacgtctcg atctacgtcg
      961 cccgcgagcg ccgcgggtgc ggcatcggca cgacgttgct tacggcctta gaagatgcgg
     1021 ctcgcgcggg cggctttcac aagctggtgc tgcacgcgct cgaccggaac gcgccggcca
     1081 agcacctgta tcgcaaagcg ggattttcgc aggtcggcgt gttcgccgag cacggcatcc
     1141 tcgacgggcg ttacgaggac gtcgtcgcga tggaaaaact cctgcgatag ctcggccgca
     1201 ccgggacggg accgtccggg cgcagcgtca ccaagggcgc gggccgaacg tccacgcccg
     1261 gttcgagcgc aaacgaaaat cgggcggtaa ccgcgcgcag cacgatcgcg gcttcgcgca
     1321 gcgcgaagtc gttgccgatg cagcggcgcg ctcccgcgcc gaacggcaga tacgaaaacg
     1381 gcgcgggttc ggcgcccagc cagcgatccg ggtcgaagcg atcgggatcg cggaaaaact
     1441 cggggcggcg gtggagcagc aacggcgcga tcagaacggt ggtcttcggc gccaccaggg
     1501 tcccgtcgga gagcgtcacc gcacgccgcg actcgcgtcc caccacccag gccggcggat
     1561 agaggcgcaa gctttcgagc acgacgcgat cgatgaagtc gccgtcgccg tcgatcgcgg
     1621 ccgccgccgt cgccgcatcg acgtccgggt gttgcgctaa caaataccac gtccagacta
     1681 gcagattggc ggtcgtctcg tgacccgcca taaaaagcgt catcacctcg tcgcggatct
     1741 gctcgtcgct gggacggtag ccggattcgg cgtcgtgtgc cgccagcagc atcgaaagcg
     1801 cgtcgtcgcc gggagtttcg cggcggaggg cgatcaacgc gtaaatgatg tcgtcgaggc
     1861 tcttcttggc ggcttgaaag cggcgcgtgg cggggagcgg cagccgccgg cgcagctcgc
     1921 cgaacgggag caatacgtac gggaattcgc gcatcatcag cccgagcgcg gcgctcacgc
     1981 gctgggcggc gtcgctggcg tcgctaccaa acagcgtgac gctcgcaatc cgcagcgtca
     2041 gtgccgtcat cgccgcatgc agatcgaacg gccggtcggc gcgaagcccg ttcgcgaagt
     2101 cgtctgccgc ggcttccatc gccgcggcgt agcgtccgat gcgctcgcga tggaaggccg
     2161 gctgcacgat ccggcgcatc tggcggtgca gcggatcttc gctcgtcagc agtccttcgc
     2221 ccaacaggtg gcgcagcatt cgcgtgccca gcgatttcga aaacgcctgc tgctgcgtca
     2281 ctagaacgtc tttgatcagc accggctcgt tgatgaagac gtagcgccgc cagggcaccg
     2341 aaaacgcgta caggttaccg tagcgcgcgg tcatttcgcg caggaacggt acgaatttca
     2401 gaaacggcat cggcatcagc cgccgcagcg tcgtcgccgt accgggcccg ggaagcttct
     2461 tgctcatccg cccgccagtt tgaccttccg gccgcgctgc gcgaaccact ccgcgatttt
     2521 ctcgcgatgg tcgccctgca actccaccac gccgttctta gccgttccgc cggttccgca
     2581 gtggcgtttg agcagcttgg cgatctcggc gagttcgggc gcacgaagac cggtcacgac
     2641 gctcaccgtg ctcgcgcggc gccgctcgcg catgatgcgc accacgccgt cgtcgggaag
     2701 actgtgcgac tgcagggacc gtcgatcggc gggcatccgc ctgcgcttcg gcaggcaccg
     2761 cgagcccaac tgcccaaccg cctgcgcgat gcaacggcgc atctgcgtct tctgcgggag
     2821 cagttccgga aacgacgcct cgtatcgctc ggccgcgcgc gacgccgcgc gagcgatcgt
     2881 gcgcgccggt tacggcgtcg tttacggcgg cggacggatc ggactgatgg gcgagctcgc
     2941 cgatgcggcg ctggccgcgg gcggcgaggt ggtcggcgtg attccggccg cgctggcgcg
     3001 cgtagaaatc gcccacgacg ggttgacgca gctgcacgtc gtcgacagca tgcacgaacg
     3061 caaggcgctg atggctcggt tgagcgacgc cttcgtggcg ctgcccggtg cgttcggcac
     3121 gatggacgaa ttctgcgaaa tcctgagttg gcgccagctc gccatccatg acaaaccgat
     3181 cgggctgctc aaccaccgcg gctacttcga ccggctgctc gacctgttcg acgcgatggt
     3241 gcgcgagggg tttcttctgc cggccaaccg gaagctggtg gtggacgccg ccgaaatcga
     3301 gccgctgctt gcggcaatga acgttaacac caagtaacct tttcaagcgg ttaccctaaa
     3361 gccgggacgg cgcacgccgc agaagcgttg accgggccat aataactctt tagactggtt
     3421 acatgggttt cggcagcctc agcctggact tcttggcgct gccgctcgag gacgcggagg
     3481 ctctcggggc cgcgctggtc gagctgcaga cgctgtccgc gcaaccgccg gtcggcccgg
     3541 aagagctcgc cgacgcccgg cggctgcagg cggcgctggc cgcttgtacg ctcgcggcag
     3601 tccgccgcga gccgctgccc gcgcacgacg tcgcgacgct caacagctac gccggcgacg
     3661 agcctccggc gctcgtgctg cggaccgacg gcaccctagc ccgcgcgggg accgatccgg
     3721 ttcgcgcggc gctcgcggcg atcgcccgcg atgccggcga aacgattgcg tgccgtgccg
     3781 gcgagctgcg ccgctgcgaa ggcgcaaact gcgggcgcct cttcgccgac gactcgcgcg
     3841 ggcggcggcg ccgctggtgc tcgatggcgc gctgcgggaa tcgcgtcaag gccgcggcgt
     3901 ttcgccgccg gccggcgtaa agccggcggg caaggtcatc gcgagattct tgaccagaaa
     3961 tgcatacacg tcggcggtca gatcgatgct ttgcgccagc gtggtactct ctccgtgccc
     4021 agccttgagc tgcacgcgca gtaagaccgg cgccggcccg ctttgcgcgg cttgcatccg
     4081 ggcggcgaat ttgaagctgt gggctgggaa aacgcggtcg tcgtggtcgg acgtgacgat
     4141 cagcgtcggc ggatacgcca cgcccgcctt cacgttggcg tacggcgagt acttgtagag
     4201 ccaggcgaag tccgccgcgc cgcacgtcga gcagccgtat tcgggaatcc agaaatttcc
     4261 gatcgtgaac ttgtcgaagc gcagcatgtc gagcacgccg acgcccggaa ccgcggctcc
     4321 gaaaagatcc ggccgctgcg tttcgaccgc gccgaccagc agcccgccgt tcgactcgcc
     4381 tttgatcgcc agcttcggcg tcgacgtgta cttttgggcg atgagatatt ccgcggccgc
     4441 gatgaaatca tcgaaaacgt gctgcttgtt gcccagcatg ccgccccgat gccaggcctc
     4501 gccgtactcc gagccgccgc gaatgttggc gaccgcgtaa attccgccca tctgcagcca
     4561 ggttgcgatg aaactcgaaa aatacggcgt catcggtatg tcgaagccgc cgtagccgta
     4621 gagaatcgtg gggttgcttc cgtccagcgc gatccccttg cgcgcggaga tcatcatcgg
     4681 gacgcgcgtt ccgtctttgc tgcgatagaa gacctctttg gtatcgtagc cggacggatc
     4741 gaacgcaatc tgcggcttgc ggtagaccgt gctcttcccc gagacgacgt cgtaccggta
     4801 gagggtcggc ggcgtcgtcc agcccgaata cgcgtagaac gtctcgcggt cgttcgagaa
     4861 gccggcgaac ccgccggcgt tgccgagccc gggcaacgcg acgtcgcgca cgaatttgcc
     4921 cgcgtaatcg tacactttga cggccgagtg ggcgtccacg aggtaagaca ggatcagccg
     4981 cttgccgacg gccgaagcgc cctgcagggc ggccgccgct tccggcacga cggtgcgcgc
     5041 ggcgtccggg cggcgaacgt cgatcgcgac gacg
//
LOCUS       DASUCJ010000889         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_635997, whole genome shotgun sequence.
ACCESSION   DASUCJ010000889 DASUCJ010000000
VERSION     DASUCJ010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635997"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..>1512
                     /locus_tag="VFU90_10635"
     CDS             68..>1512
                     /locus_tag="VFU90_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU5480281.1"
                     /translation="MATLVLVDGSSYLYRAFHALPDLRTSKGEPTGALRGVLSMLRRL
                     VIDGKPDYFGVVFDAPGKTFRDELYPAYKANRPPMPDPLIAQIAPLHEMVRANGWPLL
                     MIPGVEADDVIGTLARQAKAAGIETLISTTDKDLAQLVEPGVTIVNTMSNERYDEAGV
                     VAKFGVRADQILDFLTLTGDSVDNVPGVEKVGPKTAAKWLGQYGSVDNILAHADEIGG
                     VVGENLRRARDWLPQGRKLLTVKVDCELPMAFEDLRPQPPDAEHLREWYERYEFKSWL
                     RDAERHPDAHPPAAARAPLRTSSATGATSATRVHAPAETTVPSASRDDSPHLTKAVPR
                     HYETVQDAEALARWIASIGAAELVSVDTETTSLDPMQASLVGISLAIEPGHACYIPIG
                     HHYAGAPPQLDRDDVLAKLSPWLTDPTPRKLGQNIKYDQHVFANHGVALAGVAEDTLL
                     ESYVLEAHRPHDMDSLALRHLGLSTITYDDVT"
BASE COUNT          267 a          510 c          487 g          248 t
ORIGIN      
        1 ctgtccgtcg gtcgaatgca gcgcttgcgg gtcgatcaat gcaggaacct tgtgggtgcg
       61 ctggggcatg gcaacgctcg tccttgtcga tggttcgtcg tacctgtacc gcgcctttca
      121 cgcgctgccg gacctccgaa cgtcgaaagg cgaaccgacc ggagcgttgc gcggcgtcct
      181 ttcgatgttg cgccgtttgg tgatcgacgg caagccggac tacttcggcg ttgtgttcga
      241 tgcaccaggc aagacgtttc gcgacgagct ctatcccgcg tacaaggcca accggccgcc
      301 gatgcctgat ccgttgattg cgcagatcgc gccgctgcat gaaatggtgc gcgcgaacgg
      361 ctggccgctg ctgatgattc cgggtgtcga ggccgacgac gtgatcggca cgctcgcgcg
      421 gcaggcgaag gccgccggca tcgagacgct gatctcgacc accgacaagg acctcgcgca
      481 actcgtcgag cccggcgtga cgatcgtcaa cacgatgtcg aacgagcgct atgacgaagc
      541 aggcgtagtt gcgaagttcg gcgttcgcgc cgaccagatc ctcgacttcc tgacgctcac
      601 cggcgacagc gtcgacaacg ttcccggggt ggagaaggtt ggaccgaaga ccgcggcgaa
      661 gtggctcggc cagtacggca gcgtcgacaa cattcttgct cacgccgacg agatcggcgg
      721 cgtcgtcggc gagaacctgc gcagggcgcg cgactggctg ccgcagggtc ggaagctgct
      781 gacggtcaag gtggactgcg agctgccgat ggcgttcgag gatcttcgcc cgcagccgcc
      841 tgacgccgag caccttcgcg aatggtatga gcgctacgag ttcaagagct ggttgcgcga
      901 cgccgagcgc caccccgatg cgcatccacc cgccgcagcg cgcgcgccgc ttcgcacgag
      961 cagcgcgact ggtgcgacca gtgcgaccag ggtccatgcg cctgccgaga cgaccgtgcc
     1021 gtcggcgtca cgcgatgatt cgccgcatct gacgaaagcc gtaccgcgcc actacgaaac
     1081 cgtgcaggat gccgaagcgc ttgcgcgctg gattgcgtcg atcggcgccg ccgagctcgt
     1141 ttcggtcgac accgagacga cgagtctcga tccgatgcag gcatcgctcg tcggcatctc
     1201 gctcgcgatc gagccgggcc atgcctgcta catcccgatc ggacatcact acgcaggcgc
     1261 gccgccgcag ctcgaccgtg acgacgtgct tgcgaagctt tcgccgtggc tcaccgatcc
     1321 gacacctcgc aagctcggcc agaacatcaa gtacgaccag cacgtcttcg ccaaccacgg
     1381 cgtcgcactc gccggcgtcg ccgaggacac gctgctcgag tcgtacgtgc tggaagcgca
     1441 ccggccgcac gacatggaca gcctcgcgct gcgccacctc ggcctctcga cgatcaccta
     1501 cgacgacgtc ac
//
LOCUS       DASUCJ010000890         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_85903, whole genome shotgun sequence.
ACCESSION   DASUCJ010000890 DASUCJ010000000
VERSION     DASUCJ010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85903"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFU90_10640"
     CDS             <1..703
                     /locus_tag="VFU90_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5480282.1"
                     /translation="QIPSSGFPYWLFQGLVIENGINLMNPAGTEVYYDKPETVGALQY
                     WVDLVNKYKVHPPGIVDWGTTPKDFLEGRVAMMWTTTGNLTNIRKNAKFDFGVAMLQE
                     NKRRGSPTGGGNFYIFKKSTPAQREAAFDFIKWVTQPARAAQWGIDTGYIAVRADAWD
                     TPEMKKYVEGFPAAAVARDQLPFAKAELSTHDNQRVTKALNDGLQAALTGSKSPAAAM
                     KDAQREADRLLKPYR"
     gene            719..1594
                     /locus_tag="VFU90_10645"
     CDS             719..1594
                     /locus_tag="VFU90_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5480283.1"
                     /translation="MARTREWLYAWLLLLPAVALLALFTHYPAIANLVHSFYSTPRAN
                     HASKFIGFDNYRNLVEDPVFWQALTNNLWYALGTIPLSIALALLMAIWVNGRIAGRGV
                     LRLAYFTPTILPMIAVANIWLFFYTPEYGLLEQFLGWFGFAGHNWLGSRDTALAAVMV
                     VAIWKEAGFFMIFYLAALQGMSPQLAEAAAIEGTPRFYFFRRVTFPLLMPTTLFVLVN
                     AVINAFRMVDHIVVMTRGGPDNATELLLYYIYEIGFRFFDSGYAAALTMVLLAILAAA
                     ALLQFGFLERRTHYQ"
BASE COUNT          280 a          554 c          529 g          293 t
ORIGIN      
        1 gcagattccg tcctcgggct ttccgtactg gctgttccag gggctcgtga tcgagaacgg
       61 catcaacctg atgaaccccg cgggcaccga ggtctattac gacaagccag agaccgtcgg
      121 cgcgctgcag tactgggtcg atctcgtcaa caagtacaag gtgcatccac ccggcatcgt
      181 cgactggggc acgacgccga aggacttcct cgaaggccgc gtcgcgatga tgtggacgac
      241 gaccggcaac ctcaccaaca ttcgcaagaa cgcgaagttc gatttcggcg tcgcgatgct
      301 gcaggagaac aagcgccgcg ggagtccgac cggtggcggc aacttctaca tcttcaagaa
      361 gtcgacaccg gcgcagcgcg aggccgcgtt cgatttcatc aagtgggtca cgcagcccgc
      421 gcgggccgcg caatggggca tcgacaccgg ctacatcgcc gtgcgcgcag acgcatggga
      481 tacgcccgag atgaagaagt acgtcgaggg cttcccggcc gcggcggtcg cgcgcgacca
      541 gcttccgttc gccaaggccg agctctcgac gcacgacaac cagcgcgtca ccaaggcact
      601 caacgacggc ctccaggcag cgctcaccgg cagcaagtcg ccggcggcgg cgatgaagga
      661 cgcccagcgc gaggctgacc ggctgctcaa gccgtatcgc tgacccgtat cgccgacgat
      721 ggcgcgaacg cgcgagtggc tctacgcgtg gctgcttctc ctgcccgcgg tggcgctgct
      781 cgcgctcttc acgcattacc cggcgatcgc gaacctcgtc cacagcttct attccacgcc
      841 gcgcgcgaat cacgcgtcga agttcatcgg cttcgacaac taccggaacc tcgtcgagga
      901 cccggtgttc tggcaggcgc tgaccaacaa cctgtggtac gcgctcggca cgattccgct
      961 gtcgatcgca ttggcactgc tgatggcgat ctgggtgaac gggcggattg ccgggcgagg
     1021 cgtgctgcgg ctcgcgtact tcacgccgac gatcctgccg atgatcgcgg tcgccaacat
     1081 ctggctcttc ttctacacac ccgagtacgg cctgctcgag cagttcctcg gctggttcgg
     1141 attcgccgga cacaactggc tcggcagccg cgacaccgcg cttgccgcgg tgatggtcgt
     1201 cgcgatctgg aaggaagccg gcttcttcat gatcttctac ctcgcggcgc tgcaggggat
     1261 gtcgccgcag ctcgccgagg cggcggcaat cgagggcacg ccgcgcttct acttcttccg
     1321 ccgcgtgacg ttcccgctgc tgatgccgac gacgctcttc gtgctggtga acgcggtgat
     1381 caacgcgttc cggatggtcg accacatcgt cgtgatgacg cgcggcggcc cggacaatgc
     1441 gaccgagctg ctgctctact acatctacga gatcggcttc cggttcttcg actcgggtta
     1501 cgccgcggcg ctcacgatgg tgctgctcgc gattctcgcg gcggcggcgc tgctgcagtt
     1561 cggcttcctc gagcgcagga cgcactacca gtgagcacgc tgccggcttc cgcgggggct
     1621 gtggcgtcgc cggtgcggca cctcgtgctg aacgga
//
LOCUS       DASUCJ010000891         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1293342, whole genome shotgun sequence.
ACCESSION   DASUCJ010000891 DASUCJ010000000
VERSION     DASUCJ010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293342"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2302)
                     /gene="polA"
                     /locus_tag="VFU90_10650"
     CDS             complement(<1..2302)
                     /gene="polA"
                     /locus_tag="VFU90_10650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5480284.1"
                     /translation="MSLMLLDTYGLVYRAFFALPGLTTTKGVPINAAYGFTMMLNKLI
                     ADEKPTHVVAAFDKGMPAERVALYKEYKAQRTTMPDELRSQFALVRRILAVHRIPIVE
                     LEGQEADDVIATLARQAEESGEQTVVVTGDLDLLQIVDDRTTVLTTRRGITELGRYDP
                     SAVRERFGLEPSQLPDYRGLKGDPSDNLPGIPGVGEKTAAKLLQAAGSLEALLANPEL
                     AGNPKLRGLVERYGEQARLCRTVSIVRRDLPLRVDWEASRYETPPDPELYRLYAELEF
                     KSLLAKLQAPADLPLFEEQVTLEGSYRTYVASTDPPEFARLGDELRALADKPRAALAV
                     LGDALGVSAEAGTGIAFASGALDYPAVREALGNLLRGGAVGAYDAKRACRLLAERGIA
                     NVRFSDDAMIAAHLLDPARGFADVEDAAAVFLQSGVAPEAAAHADATLRLIEATRAQL
                     AERDQLRLYEAVEVPLAPVLARMEAAGVAIDPRELHAVGDEVDATAARLQKNIYDFAG
                     EAFNIGSPQQLGKVLFDKLQIPGGKKTKTGWATGVEVLQGLAREYPICSLVLEWREVT
                     KLKNTYVDVIPQLIDPRDGRLHTVFNQTATATGRLSSTNPNLQNIPVRGELGRRIRRA
                     FVARDAGYVLLAADYSQIELRLMAHLSGDEAMRSAFEEGQDIHDFTARQIFGIGAGDA
                     VDANQRRMAKSVNFGLLYGMSDFGLAQRLEIGRAEAKEITAAYFARFPSVRAYVARTL
                     EEGRRNGYVSTLLGRRRYMPGLTSGNY"
BASE COUNT          381 a          821 c          791 g          419 t
ORIGIN      
        1 tgtagttgcc cgacgttagg cccggcatgt agcggcgccg cccgaggagc gtcgagacgt
       61 agccgttacg ccgcccctct tcgagcgttc gcgcgacgta cgcgcgaacc gacggaaagc
      121 gcgcgaaata cgcagccgtg atttccttcg cttcggcgcg tccgatctcg agccgttgcg
      181 cgagcccgaa atccgacatg ccgtacagca gcccgaagtt cacgctcttg gccatccgcc
      241 gctggttggc gtcgaccgca tcgccggcgc cgatcccgaa aatttgccgc gcggtgaaat
      301 cgtggatgtc ttgcccttct tcgaacgcgc tgcgcatcgc ctcgtcgccc gagagatgcg
      361 ccatcaagcg cagttcgatc tggctgtagt ccgcggccag cagcacgtag ccggcgtcgc
      421 gcgcgacgaa cgcgcggcgg atccgccggc ccagctcgcc gcgcaccgga atgttctgca
      481 ggttcggatt cgtcgacgag agccggccgg tggcggtggc cgtctgattg aaaacggtgt
      541 gcaagcgccc gtcgcgcgga tcgatcagct gcgggatgac gtcgacgtag gtgttcttga
      601 gcttggtgac ctcgcgccac tccaagacca gcgaacaaat cggatactcg cgcgcgagac
      661 cctgcagcac ttctacgccg gtggcccagc cggttttggt tttcttgccg ccgggaatct
      721 gcagcttgtc gaacagcacc tttcccagct gctgcggcga accgatgttg aacgcttcgc
      781 ccgcaaaatc gtagatgttc ttctgaaggc gcgccgccgt cgcgtcgact tcgtcgccga
      841 cggcgtgcag ctcgcgcgga tcgatcgcga cgccggcggc ttccatccgc gccagtaccg
      901 gcgccaacgg aacctcgacc gcttcgtaca gccgcagctg atcgcgctcg gccagctgcg
      961 cgcgggtcgc ttcgatcagc cgcagcgtcg cgtcggcatg cgccgcggct tccggtgcta
     1021 cgccgctttg cagaaaaacg gccgccgcat cctcgacgtc ggcaaagccg cgcgccgggt
     1081 ccagcaagtg cgcggcgatc atcgcgtcgt cggagaagcg cacgttcgcg ataccgcgct
     1141 ccgccagcag ccggcacgcg cgtttggcgt cgtaggcgcc caccgcgccg ccgcgcagca
     1201 gattgccgag cgcctcgcgc acggccggat aatcgagcgc tccgctcgcg aacgcgattc
     1261 cggttcccgc ctccgcgctc actcccagcg cgtcacccag caccgccagc gccgcgcgcg
     1321 gtttgtccgc cagcgctctt agctcgtcgc ccagccgggc gaactcgggc ggatcggtcg
     1381 acgcgacgta cgtgcgatag ctgccctcga gggttacctg ctcctcgaaa agcggaagat
     1441 ccgcgggcgc ttgaagcttt gccagcagcg atttgaactc gagttcggcg tacagccggt
     1501 agagctccgg atcgggcggc gtctcgtagc ggctcgcttc ccaatcgacg cgcagcggca
     1561 gatcgcgccg cacgatcgat accgtgcggc acaatcgcgc ctgctcgccg tatcgttcca
     1621 ccagaccgcg gagtttcgga ttgcccgcca gctccggatt ggccagcagg gcttccagcg
     1681 atccggcggc ctgcaagagt ttagcggcgg tcttttcgcc gacgcccgga attccgggaa
     1741 ggttgtcgga cgggtcgcct ttgaggccgc ggtagtcggg cagctgcgac ggttcgagcc
     1801 cgaagcgttc gcggaccgcg gacgggtcgt agcgtccgag ttcggtgatc ccgcggcggg
     1861 tcgtcaagac cgtcgtacga tcgtcgacga tctgcagcag atcgagatcg ccggtcacga
     1921 cgaccgtctg ctcgccggac tcctcggcct gccgcgcgag cgtggcgatc acgtcgtcgg
     1981 cctcctgccc ttccagctcg acgatcggga tgcgatggac ggccaggatc cgccggacca
     2041 gcgcgaactg gctgcgcagc tcgtcgggca tcgtcgtccg ctgcgccttg tattccttat
     2101 aaagggcgac ccgctcggcg ggcatgcctt tgtcgaaggc ggcgacgacg tgcgtcggct
     2161 tctcgtcggc gatgagcttg ttgagcatca tggtgaagcc gtaggccgcg ttgatcggca
     2221 cgcccttggt cgtcgtgaga ccgggcaggg cgaagaaggc gcgatacacc agaccgtagg
     2281 tgtcgagcag catcaagctc ataaaggtaa ggagcctatc gtaccacgac gtcggaggag
     2341 ccggcgctta cgctgccgtc gtcggcgatg tcgagcaccg agacggtata acgcccgctc
     2401 tccgcgggca tc
//
LOCUS       DASUCJ010000892         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_743149, whole genome shotgun sequence.
ACCESSION   DASUCJ010000892 DASUCJ010000000
VERSION     DASUCJ010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743149"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1251
                     /locus_tag="VFU90_10655"
     CDS             <1..>1251
                     /locus_tag="VFU90_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5480285.1"
                     /translation="AAYKAQNLIPGDDFRLLFDIDSGKLGTSVVSYRAKADEDGYFLL
                     LTSPEIKRDPAERPKKTVLFVVDRSGSMTGAKIEQAKGALKFVLNNLREGDTFNIIAY
                     DSAVESFRPELQKFDDETRKAALGFVEGIYAGGSTNIDGALTAALAQLKDSSRPNYVL
                     FLTDGLPTAGEQNEMKIVANSESHNRVRARVVAFGVGYDVNSRLLDKLARQNSGQSEY
                     VRPDENIEERVSRLYNKIEAPVMTDVAIRFALDEQRAEAGEPVNRLYPKKVYDLFEGD
                     QLVLVGRYKRAGAAKVTVTGSVAGRQESFDFPATLTDKSSDETFAFVERLWAMRRVGE
                     IIDELDLQGQNPELVKELVELSTRHGIMTPYTSFLADENVDLRQLSRNAGTAGDRLNA
                     LSAVEGRAGFQQREFKGRLQGVKQA"
BASE COUNT          271 a          385 c          406 g          189 t
ORIGIN      
        1 ggccgcctac aaggcccaga acctgatccc cggcgacgat ttccggctgc tgttcgacat
       61 cgactcgggc aagctcggca cgagtgtcgt ctcgtaccgc gcgaaggccg acgaagacgg
      121 ctactttctg ctgttgacca gccccgaaat caagcgcgat ccggccgaac ggccgaagaa
      181 aacggtgctg ttcgtggtcg atcgctcggg cagcatgacg ggcgcgaaga tcgagcaagc
      241 caaaggcgcg ctcaaattcg tgctcaacaa cctccgcgaa ggcgacacgt tcaacatcat
      301 cgcctacgac agcgcggtcg agtcgttccg gcccgaactg caaaagttcg acgacgaaac
      361 gcgcaaggcg gcgctgggct tcgtcgaggg gatttacgcc ggcggcagca cgaacatcga
      421 cggcgcgttg acggcggcgc tggcccagtt aaaagattcg agccggccga attacgtgct
      481 gttcctcacc gacggcctgc ccacggcggg cgaacagaac gaaatgaaga tcgtcgccaa
      541 cagcgaatcg cacaaccgcg tgcgagcccg cgtggtggcc ttcggcgtcg gctacgacgt
      601 caacagccgg ctgttggaca agctggcgcg gcagaactcg gggcagagcg aatacgtgcg
      661 gcccgacgag aacatcgagg agcgcgtcag ccggctgtac aacaagatcg aggcgccggt
      721 gatgaccgac gtggcgatcc gtttcgccct cgacgaacag cgcgcggagg ccggcgagcc
      781 ggtcaatcgc ttgtacccca agaaagtcta cgatctgttc gaaggcgatc aactcgtgct
      841 ggtcggccgc tacaagcggg cgggcgccgc gaaagtcact gtcacgggca gcgtcgcagg
      901 ccgccaggaa agcttcgatt tccccgcgac attgaccgac aagagcagcg acgagacgtt
      961 cgccttcgtc gagcggctgt gggccatgcg ccgcgtgggc gagatcatcg acgaattgga
     1021 tctccagggc cagaacccgg aactggtcaa ggagctggtc gaactctcga cgcggcacgg
     1081 catcatgacg ccctacacgt cgttcttggc cgacgaaaac gtcgatctcc gccagctttc
     1141 gagaaatgcc ggaacagctg gcgacaggtt gaatgcgttg agcgccgtcg aaggccgagc
     1201 gggcttccag cagcgcgagt tcaaagggcg attgcagggc gtcaagcaag c
//
LOCUS       DASUCJ010000893         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_651542, whole genome shotgun sequence.
ACCESSION   DASUCJ010000893 DASUCJ010000000
VERSION     DASUCJ010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651542"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..>2298)
                     /locus_tag="VFU90_10660"
     CDS             complement(343..>2298)
                     /locus_tag="VFU90_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5480286.1"
                     /translation="RRDGMLYVDTMMYPWFSFNEYLCFRVDLQGSGDSDGVITDEYTE
                     DELASCVQVIEQIAALPFCDGNVGMMGKSWSAINSLMVAARDDCPAALRAIVFCCGTD
                     DRFNDDVHYMGGAMMYDQPSWAGSMWGWLPMPPDPLVVGARWKEMWRERVRSLDFWFA
                     TWAAHQTRDAYWRRTSVRDHYERVKVPVFVISGWWDGYKNPAERAVRTLGGMGKPVAG
                     ILGPWGHKYPFDGYPGPRVDWLRYVVPHWWDRWLKGKTPDESSAWPQLAVWLGESREP
                     TRIPDYVERGTWVAEDHAWQQRVRPYALHCAPGNALSADPVAQHQAYRISSDVLAGTA
                     MLESTSWGECENDDLPRDMRADDRRSLYFDTPPLERDLACFGYPEVTLDLRCRSRGPA
                     AVAVRLCEVAPSGRSHLVTYRFWNLGYLHGELGAPQAVPSGRFAVTLPLNVMGHTFKR
                     GWKIRLAISPAWVPTMWRAPEPAEVTLRAGGDARSALLLPVRPARAEDARVEAALGRP
                     VTAHVNPQLYAPIEATRREAVNRRTAAAVEIDGKPGVAVHKLMDAGSYVYGGMLEHLL
                     VDSSATEEFRTVAGDPLPAEMTTRYESRLARGDWSVRAVTTTRVWSARTPAGGAEFRY
                     SAEVETFEGEQPFERRRVEGSIPRTWV"
BASE COUNT          404 a          799 c          721 g          374 t
ORIGIN      
        1 aatccaacga acacggattc acgcgtgctc cgccgtgcgc cgggcagtgc ccctgcacgt
       61 tgacgatcgg cgccgtgccc tgcgcgggcg ctaccggacc gatctgcgac tgcgacccgc
      121 cgcagcctgc gagcatggca ccggccgcga ttgcgctaac ggcgatccgg gccaagtaag
      181 atgtagacac ccttgatctc cttcgagcag tattccgtaa gtagaacgtc tagctcgtgc
      241 taggctgcca tcttaccact gtagaatcgg cccagtcgtc atgcacttgc aactgtacag
      301 ttactgtagg acaaactgta tacaagtgta gtttaggcaa cgttagaccc aagttcgcgg
      361 aatcgacccc tcaacgcgtc gccgttcgaa cggctgctcc ccttcaaagg tttcgacttc
      421 ggcggaatag cggaactcgg cgccgccggc gggcgtccga gcgctccaaa cgcgcgtcgt
      481 ggtgaccgcg cgcacgctcc agtcgccgcg cgccagccgc gattcgtaac gggtggtcat
      541 ttcggccggc aacggatcgc cggcaacggt gcgaaactct tcggtggcgc tcgagtctac
      601 cagcaagtgc tcgagcatcc cgccgtagac gtaactgccc gcgtccatca atttatgtac
      661 ggcgacgccg ggcttaccgt cgatctcgac ggcggcggcg gtccgccggt tgacggcctc
      721 gcgccgcgtc gcctcgatcg gagcgtagag ttgcgggttc acgtgggcgg tgaccgggcg
      781 cccgagcgcc gcctcgaccc gcgcgtcttc ggcgcgggcg gggcggacgg gcaagagcag
      841 cgcggatctc gcgtcgccgc cggcgcggag cgtaacctcc gcgggctccg gtgcgcgcca
      901 catcgtcggg acccatgccg gcgagatcgc cagccgaatc ttccatccgc gcttgaacgt
      961 gtggcccatc acgttcaacg gcagggtcac cgcaaagcgt ccgctcggaa cggcttgcgg
     1021 cgctccgagc tcgccgtgga gatagccgag gttccaaaag cgataggtca cgagatgcga
     1081 tcgtcccgac ggtgcgacct cgcatagccg caccgcgacc gcggccggcc cgcggctgcg
     1141 gcacctcaaa tcgagcgtaa cctcgggata gccgaaacac gcgagatcgc gttccagcgg
     1201 cggcgtatcg aaatacagcg accgccggtc gtcggcccgc atgtcccgcg gcagatcgtc
     1261 gttttcgcat tcgccccacg acgtcgactc gagcatcgcc gtgccggcga ggacgtccga
     1321 ggatatccga tacgcctgat gctgcgcaac cggatccgcc gaaagcgcgt ttccgggagc
     1381 gcagtgcagc gcatagggcc gcacgcgctg ctgccacgcg tggtcctccg ccacccacgt
     1441 gccgcgctcg acgtagtcgg gtatccgggt cggctcgcgc gactcaccca gccagaccgc
     1501 gagctgcggc caggccgacg attcgtccgg cgtctttcct tttaaccagc ggtcccacca
     1561 gtgcgggacg acgtagcgca gccaatcgac gcgcggaccg ggatagccgt cgaacggata
     1621 cttgtgcccc cacgggccga gaataccggc gaccggtttc cccatgccgc ccaacgtgcg
     1681 caccgcgcgc tcggcgggat tcttgtagcc gtcccaccag cccgagatga cgaacaccgg
     1741 caccttcacg cgttcgtagt ggtcgcgcac gctcgtacgg cgccagtagg cgtcgcgcgt
     1801 ttggtgcgcc gcccaggtcg cgaaccagaa gtcgagcgaa cgcacgcgct cgcgccacat
     1861 ttctttccag cgtgcgccga cgaccagcgg atccggcggc atcggcagcc agccccacat
     1921 cgagccggcc cagctcggct gatcgtacat catcgcaccg cccatgtagt gcacgtcgtc
     1981 gttgaagcgg tcgtcggtac cgcagcaaaa gacgatcgcc cgcagggcgg ccggacagtc
     2041 gtcgcgcgcg gcgaccatca gcgagttgat cgcgctccac gatttgccca tcatcccgac
     2101 gttgccgtcg caaaacggca gcgccgcaat ttgctcgatc acctggacgc acgacgcgag
     2161 ctcgtcttcg gtatattcgt cggtgatgac gccgtcggag tcgccgctgc cctgcaggtc
     2221 gacgcgaaag cacaggtact cgttaaacga aaaccacgga tacatcatgg tgtcgacgta
     2281 cagcatcccg tcgcggcg
//
LOCUS       DASUCJ010000894         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_896082, whole genome shotgun sequence.
ACCESSION   DASUCJ010000894 DASUCJ010000000
VERSION     DASUCJ010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896082"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFU90_10665"
     CDS             <1..520
                     /locus_tag="VFU90_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5480287.1"
                     /translation="VVAPALYRHEKKIVLPVFAFATLLFLSGVCLSFFVVLPLAVEWL
                     MSFQTQALEPMITATEYFGFATSMALAFGLCFELPIVILALAALGIVTPQFLNKYRRH
                     AIVICVIIGAFLTPGDLVWTTIAMAVPLYLLYEVSVLLTYGVHKRRLRRQAQREADAA
                     EVEGAERRGATA"
     gene            517..>1554
                     /locus_tag="VFU90_10670"
     CDS             517..>1554
                     /locus_tag="VFU90_10670"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEU5480288.1"
                     /translation="MIRRTIAAVAVLALPLGGAAAAQGVPTAPPTPPPIFPDTTQRIY
                     RQPRRGDTTQTDTSRVKTDLVKWNEPDSVMNALMSRTGYVTTRYQGNTAVFNTQSHAL
                     QLSGQSAVQREQTVLVSDTIEYNDSSRIVTARTAPNDTTVLRDPSAQTSDLVTLGFLE
                     YDIGNHKGIANRLSTSSAQAGQTWFVGGERGAVAGDTTGHGHNTSYALDGSVTSCDLP
                     DPHYHFQSKEIKMVTRRLLVARPAVLYIQDIPVLWLPFIFQDMRTGRRSGIIPPRFGL
                     SDIVRTSASYQREVDNLGYYFAINDYTDATASLDWRSGNNAQNGNIGWTRWNGEFEYR
                     WLDRFLAGRIGV"
BASE COUNT          255 a          542 c          497 g          260 t
ORIGIN      
        1 ggtcgtcgcg ccggcgctgt acaggcacga gaagaaaatc gtgctcccgg tgttcgcgtt
       61 cgcgacgctg ctgtttctct ccggcgtgtg cctgtcgttc ttcgtcgtgc tgccgctcgc
      121 ggtggagtgg ctgatgagct tccagacgca ggcgctcgag ccgatgatca cggccaccga
      181 gtacttcggc tttgcgacga gcatggcgct ggccttcggc ctctgcttcg agttgccgat
      241 cgtgatcctg gcgctggctg cgttaggcat cgtgacgccg cagttcctca acaagtaccg
      301 gcggcacgcg atcgtgatct gcgtgatcat cggcgcgttc ctcacgccgg gcgacctggt
      361 ctggacgacg atcgccatgg cggttcctct gtacctgttg tacgaggtca gcgtcctcct
      421 cacgtacggc gtgcacaagc ggcgtttgcg gcggcaggcg cagcgtgagg cggacgcggc
      481 cgaggtggag ggcgcggagc ggcgcggagc gacggcgtga tccggcgcac gatcgcggcc
      541 gtggccgtgc tcgcgctgcc gttaggcggc gccgcggcgg cacagggcgt tcccaccgca
      601 ccgccgacgc cgccgccgat ctttcccgac accacgcagc gcatctaccg ccagccgcgt
      661 cggggcgaca ccacccagac cgacacgtcg cgcgtcaaga cggacctcgt caagtggaac
      721 gagcccgact cggtcatgaa tgcgctcatg agccgcacgg gctacgtcac cacgcggtac
      781 cagggcaata ccgccgtctt caacacgcag agccacgcgc tgcaactgtc ggggcagagc
      841 gccgtccagc gcgagcagac cgtgctcgtc tccgacacca tcgagtacaa cgattcctcg
      901 cgcatcgtca cggcccgcac cgcgcccaac gacacgaccg tcctgcgcga tccgagcgcg
      961 cagacgtcgg atctcgtcac gttgggcttt ctcgagtacg acatcggcaa tcacaagggc
     1021 atcgccaacc ggctcagcac gtcgtcggcg caggcgggcc agacctggtt cgtgggcggc
     1081 gaacgcggcg cggtggccgg cgacaccacc ggccacggac acaacacgtc gtatgcgctg
     1141 gatggctcgg tcaccagctg cgacctgccc gatccgcact accacttcca gtcgaaggaa
     1201 atcaagatgg tcacgcggcg gctgctcgtt gcgcggcccg ccgtgctgta catccaggac
     1261 attcccgtcc tctggctgcc gttcattttc caggacatgc gcaccggacg gcgcagcggc
     1321 atcatcccgc cgcgttttgg cctctccgac atcgtgcgca cctcggcgtc gtaccagcgc
     1381 gaagtcgaca acctcggcta ctacttcgcc atcaacgatt acaccgacgc cacagcgtcg
     1441 ctggattggc ggagcggcaa caacgcgcag aacggcaaca tcgggtggac ccgctggaac
     1501 ggcgaattcg agtaccggtg gctcgaccgc ttcctcgccg gtcgcattgg cgtc
//
LOCUS       DASUCJ010000895         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1186233, whole genome shotgun sequence.
ACCESSION   DASUCJ010000895 DASUCJ010000000
VERSION     DASUCJ010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186233"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..510)
                     /locus_tag="VFU90_10675"
     CDS             complement(298..510)
                     /locus_tag="VFU90_10675"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEU5480289.1"
                     /translation="MIQRDKPKAVSALVPGGLVGAADYLSIARSTLCALRVNDPSFPK
                     PVQITARRIGFYARDLDSWLEARRAA"
BASE COUNT          170 a          323 c          355 g          193 t
ORIGIN      
        1 gatcttgttg acgcgccgct cgccggcgat gccgtcgtgg acgagcgagt aggtcttgcc
       61 cacgacggcg ggattcgtgc tcgtgattac cgacagcgcc gcttccgggc gcacgatctt
      121 gggctcgcgc acgagtgcga tgcttcgtat ggttggtaat ttcgcgtcca taattttcag
      181 accttcaatg tcgtgttgtc gatcggccgg agcgtggaga gcgccccggc cgtgcctctt
      241 ctgcgcgtca gggatcgaga ttccgcgcga actcccgcac cgcacgggcg gggccgttca
      301 cgccgccctc cgggcttcga gccacgagtc caaatcccgg gcgtagaacc cgatgcggcg
      361 cgccgtgatc tgcaccggct tcgggaacga gggatcgttg acccgcaggg cgcaaagcgt
      421 cgaccgggcg atggacaagt agtcggcggc gccgaccaag ccgccgggga cgagtgcgga
      481 taccgccttg ggtttgtcgc gttgaatcat ggtgctgctc ctgtatatgc gcggcggggc
      541 gtaccggccc gcctcgggtg agcgcaaagg tgcgctcgct ctgttccttg ggggtacttc
      601 ggctgcaagc aacgacgagg gcgccataga gcgcacgcgc gggacgcgcg gcgtcgcttg
      661 ctgctactgg atcggtgggt gggtaggtag gtgggtagct aggtttcgcg atctgcgtcg
      721 cgtctacacc ggtactgcca ccgtgccttg cgcgcgggtc gttctcgcca caggtgcttc
      781 ggtactacga cgcccatcat acgttgcaat tttctaagcg gacattgatg gggccagtcg
      841 cgccgatcaa tatgcccatg ctacggagcg aatttttcaa tgcaaccccg aataccccgg
      901 aatcgagcgc gacctgacga acggcaggtc agccgcacgc acaagcgatc cgcgacgagc
      961 tgatgcaggt cgggcaagcg ccggcggtga cgacgccgac gattcaggtg acgccgcccg
     1021 ccgctgcgca ggcaacgccc g
//
LOCUS       DASUCJ010000896         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_483305, whole genome shotgun sequence.
ACCESSION   DASUCJ010000896 DASUCJ010000000
VERSION     DASUCJ010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483305"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1317
                     /locus_tag="VFU90_10680"
     CDS             <1..>1317
                     /locus_tag="VFU90_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5480290.1"
                     /translation="PILGRGVSDPAIAQTPIDWIEIVTAPGSHYAIAFVSPGTDPRLR
                     VYAAPVDAVQDGKTVWRPVAATYDDQYIGGLYSGGDSDYPVALAGDTLYWLSRKDAPR
                     GRILKLDLAHPRSKPEIVVPQGDLPISEVYPARDAIYWRVNDAGVNSVSRLRFARGAK
                     PEALRLPYAADVVDVATDRETDATALLAVSWLRSNAYLGVDAKTGAVTDTGLQPAGPY
                     DHLDDLVVDEVKVKSWDGVEVPLSIVHKKGLKLDGNNPTEINGYGAYGVSSSPRSDPA
                     WRAWYERGGVQAVAHVRGGGEYGEAWHQAGFQATKPNTWKDFIACAQYLVDHKYTNPH
                     KLIGSGASAGGILIGRAIEQRPDLFAGALMIFPAADTLRFETTANGPRNVAEFGSVKT
                     EAGFRALYAMSPYANVKDGVKYPAALVNASINDPRVAAWIPAKLVAR"
BASE COUNT          245 a          429 c          417 g          226 t
ORIGIN      
        1 ccgatcctcg gacgcggcgt cagtgacccg gcgatcgcgc agacgccgat cgactggatc
       61 gagatcgtca ccgcgcccgg ttcgcactat gcgatcgcct tcgtctcgcc tggcaccgac
      121 ccgcgtttgc gcgtctacgc agcgccggtc gatgccgtcc aggatggcaa gaccgtttgg
      181 cggcccgtcg cggcgacgta cgacgatcag tacatcggcg gcctgtacag cggcggcgat
      241 tcggactacc cggtcgctct ggccggagac acgctgtact ggctgtcgcg caaggacgca
      301 ccgcgcggcc ggattctgaa gctcgatctt gcgcacccgc gatcgaagcc ggaaatcgtc
      361 gtaccccagg gcgacctgcc gatcagcgag gtctaccccg ctcgcgatgc gatctactgg
      421 cgtgtcaacg atgcgggcgt caacagtgtt tcgcgactgc gcttcgcgcg tggcgccaaa
      481 ccggaagcgt tgcgtctgcc ctatgcggcg gatgtcgtcg acgtggcaac cgatcgcgaa
      541 accgacgcga cggcccttct tgcagtgagt tggctgcgtt cgaatgccta tctcggcgtc
      601 gacgcaaaaa ctggcgcggt caccgacact ggcctgcaac cggcaggacc ctacgatcat
      661 ctcgacgatc tggtcgtcga cgaagtcaaa gtcaagagct gggacggcgt tgaagtccca
      721 ctatcgattg tccacaagaa aggactgaag ctggacggca acaatcccac ggagatcaac
      781 ggttacggcg cctacggcgt gagctcgtcg ccgcgctccg atccggcctg gcgtgcttgg
      841 tatgagcgcg gcggtgtgca agccgtagcc cacgtgcgcg gcggcggcga gtatggcgag
      901 gcgtggcacc aggccggatt ccaggcgacc aagccgaata cctggaagga ttttatcgcc
      961 tgcgcccagt atctcgtcga ccacaagtac accaacccgc ataagctgat cggatccggg
     1021 gcaagcgccg gcggcatcct gatcggacgg gcgatcgagc agcggccgga cctgttcgcg
     1081 ggcgctttaa tgatatttcc agctgccgac acgctgcgtt tcgaaacgac cgccaatggg
     1141 ccccgcaatg tcgccgaatt cggcagtgtc aagaccgagg ccggtttccg cgcgctgtat
     1201 gcgatgagtc cctacgccaa cgtcaaggat ggcgtgaagt atccggccgc gctcgtaaac
     1261 gccagcatca acgatccacg cgtcgctgcc tggataccgg ccaagctcgt cgcgcgc
//
LOCUS       DASUCJ010000897         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1262834, whole genome shotgun sequence.
ACCESSION   DASUCJ010000897 DASUCJ010000000
VERSION     DASUCJ010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262834"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..476
                     /gene="rpsQ"
                     /locus_tag="VFU90_10685"
     CDS             162..476
                     /gene="rpsQ"
                     /locus_tag="VFU90_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5480291.1"
                     /translation="MEQHNVEAPAGAAAETSERASDRGSRRVKQGRVASSKMDKTIVV
                     VAESRVPHPTYGKIVRKSTRFKAHDERNEANVGDLVRIMECRPISREKRWRLVEIVER
                     AK"
     gene            473..850
                     /gene="rplN"
                     /locus_tag="VFU90_10690"
     CDS             473..850
                     /gene="rplN"
                     /locus_tag="VFU90_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5480292.1"
                     /translation="MIQQETRLKVADNSGARELLVIHISGGSRHAYAHVGDIVVGTIK
                     SAIPGAAVKKGQVVKAVVVRTSAPIRRGDGSVVRCDDNACVIIKGEKDNLDPRGTRVF
                     GPVMRELRDRGFLKIASLAPEVL"
     gene            855..1211
                     /gene="rplX"
                     /locus_tag="VFU90_10695"
     CDS             855..1211
                     /gene="rplX"
                     /locus_tag="VFU90_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5480293.1"
                     /translation="MLKSRILKGDTVVIRRGREKGKRGVVKAVQAQGGTATVEGLNIV
                     KRHTKQGRQSGNMGGMQTGGIIEKEAALPLSALQYVCEKCKLPVRLRHSRTSEGVLRR
                     VCARCGEPARESAKGA"
     gene            1216..>1751
                     /gene="rplE"
                     /locus_tag="VFU90_10700"
     CDS             1216..>1751
                     /gene="rplE"
                     /locus_tag="VFU90_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5480294.1"
                     /translation="MTSRLRERYEGQVRKQLQDRFGYQNANQIPKFSKVVVNMSVGEA
                     IANPKSLDAAVAELAAITGQKAIVTKARKSIAAFKLREGMNIGAKVTLRGQRMYVFLD
                     KLFNIVLPRIRDFRGLPAKSFDGRGNYNLGLREQLVFPEINYDKVDKARGMDITIVTT
                     AKTDEEASEFLVAMGLPL"
BASE COUNT          373 a          514 c          588 g          276 t
ORIGIN      
        1 gttgcagcag aaggcccggg ataccaaggc ggagctcttc aatctgcgct tcgcgttgcg
       61 aaccgggcat ctcgccgact tcagcaagat ccgagcgatg cgtcgcgtgt acgcccaaat
      121 ccaaaccgtc atctccgaga agcgcatcgc gcagggacag catggagcag cataacgtag
      181 aggcgcctgc gggcgcagcc gccgagacgt ccgaacgggc gagcgaccgg ggttcgcggc
      241 gcgtcaaaca aggccgggtc gcctcgagca agatggacaa gaccatcgtc gtcgttgccg
      301 aaagccgcgt cccgcatcct acctatggga agatcgttcg gaagtcgacg cgcttcaaag
      361 cccacgacga acgcaacgaa gcgaacgttg gcgacttggt tcgcatcatg gagtgccgtc
      421 cgatctcgcg cgagaagcgg tggcgtttgg tcgagatcgt ggagcgtgcg aaatgattca
      481 acaggaaacg cggctcaagg ttgccgacaa ctcgggcgcg cgggaactgc tcgtgatcca
      541 catttcgggc gggagccgtc acgcctacgc ccacgtcggc gacatcgtcg tggggacgat
      601 caagagcgcc atcccgggtg ccgccgtaaa gaaagggcag gtcgtcaaag cggtggtcgt
      661 ccggacgagc gcgccgattc ggcgcggcga cggttcggtc gtgcgctgcg acgacaacgc
      721 ctgcgtcatc ataaaaggcg aaaaggacaa cctcgatccc cgcggcacgc gcgtcttcgg
      781 gccggtcatg cgcgagctgc gcgaccgagg gttcttgaag atcgcttcgc tggcaccgga
      841 ggtgctgtag aactatgttg aagtcacgaa ttctcaaagg cgacacggtc gtgattcgcc
      901 gcggccggga gaaaggcaaa cgcggcgtcg tcaaggcggt tcaagcgcag ggcggcacgg
      961 cgacggtcga aggcctgaat atcgtcaagc ggcacaccaa gcagggccgt cagtcgggca
     1021 atatgggcgg tatgcagacg ggcggcatca tcgagaaaga ggcggcgctg ccgctctcgg
     1081 cgctgcagta cgtatgcgag aaatgcaagc ttccggtgcg tctgcggcac agccggacgt
     1141 ccgaaggcgt ccttcggcgc gtgtgcgctc gttgcggcga gccggcgcgc gaatcggcga
     1201 agggagcgta gtcgaatgac gtctcgtttg agagaacgct acgaaggcca ggtccgcaaa
     1261 cagctgcagg accgtttcgg ctaccagaac gcaaaccaga tcccgaagtt ctcgaaggtc
     1321 gtcgtcaaca tgagcgtcgg cgaagcgatc gccaatccca aatcgctcga cgcggcggtc
     1381 gccgaacttg cggcgatcac gggccagaaa gcgatcgtca cgaaagcgcg caagtcgatc
     1441 gccgctttca agctgcgcga ggggatgaac atcggggcca aggtgacgct acgcggccaa
     1501 cgcatgtacg ttttcctcga caagctcttc aacatcgtgt tgccgcgcat tcgcgatttc
     1561 cgcggcctgc cggccaagtc gttcgacggg cgggggaact acaatctggg gctgcgcgag
     1621 cagctggtgt ttccggagat caactacgat aaggtcgaca aggcgcgcgg aatggacatc
     1681 accatcgtca cgacggcaaa gaccgacgaa gaggcgtcgg agttcctggt cgcgatgggc
     1741 ctgccgctgc a
//
LOCUS       DASUCJ010000898         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1308477, whole genome shotgun sequence.
ACCESSION   DASUCJ010000898 DASUCJ010000000
VERSION     DASUCJ010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFU90_10705"
     CDS             <1..480
                     /locus_tag="VFU90_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5480295.1"
                     /translation="GGYRAGVVRELIGLAAILIAWLLAGAFAGPFSDSLGIQLGVSVP
                     VAHLIGFWLLFLVVFAAVRAGGWLLEKFASLPIIKIVSGIGGGIVASAKAVLLLWLLL
                     FVALFFPISADVRAAMRASPSARAIDALDTPAYAMLESALPRTVRVVAGSILKHHRL"
     gene            complement(627..>1763)
                     /locus_tag="VFU90_10710"
     CDS             complement(627..>1763)
                     /locus_tag="VFU90_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480296.1"
                     /translation="GGGPLGYPKTLTDDPLMGALSDAAKAGADELLLGSVIQASGGSV
                     YYRLSLYRVAPMAFIGSQVFSQPYPGNPGALAAGLSSNLAALSAGHRGTGTIYSTTDG
                     TKADTGTEEGFSVGDTFTVTRHGQLMADATISSITDDEAWLSFSNAVAGYQPAVGDNL
                     TSTKSMPPLPPPPGHSGFNVLALLAAAGGALLAIGHHGQPAVPCLSCFTAPPSSLPYV
                     ILNFQVLGQPPTGSITFTFSQAVNATSQTGISGNINYAEFTLQPIGGTATTPPAPLSS
                     FGSVAFDSTGTILTLGENGSGLVTGEGYQITFTTLVTSTQNQTLGPPFTTGMQTFSIF
                     RRSSAVAHPKLGPVAPGPVPGKPGNPKNPPPAPKPPPANPPIPH"
BASE COUNT          261 a          581 c          611 g          310 t
ORIGIN      
        1 ggcggctatc gcgccggcgt cgtccgcgaa ctcatcggcc tcgccgcgat cctcatcgcg
       61 tggctgctcg ccggcgcgtt cgcggggccg ttctccgaca gcctgggcat ccaactcggc
      121 gtctcggtgc cggtcgctca cctcatcggc ttctggctgc tcttcctcgt cgtcttcgca
      181 gccgtgcgcg caggcggatg gctgctcgag aagttcgcgt cgctgccgat catcaagatc
      241 gtcagcggca tcggcggtgg gatcgtcgca tctgcgaaag ccgttctgct gctgtggctg
      301 ctgctgttcg tcgcgctctt cttcccgatc tcggcggacg tgcgcgccgc gatgcgcgcg
      361 tcaccgagcg cacgcgcgat cgacgcgctc gacacgcccg cctacgcgat gctcgagagc
      421 gcgctgccgc gcaccgtccg cgtcgtcgcc ggatcgatcc tcaagcacca ccggctctga
      481 caccaagagt tgtagcggtc gacgtttatc gtcgaccgcc gcggtcttgc attagtggga
      541 gcgcagacac gggagttcta gtgcaaggcc gcggcggtcg accgtgaagg tcgaccgcta
      601 cagccctagc ctcgccgagt cttgttctag tgcgggatcg gcggatttgc cggcggcggt
      661 tttggcgccg gcggcgggtt cttgggattc cctggtttgc ccggaaccgg acccggggcg
      721 accggaccga gtttcggatg agcgaccgcc gaggagcgcc ggaagatcga gaacgtctgc
      781 atcccggtcg tgaacggcgg tccaagcgtc tggttctgcg tgctcgtgac cagcgtcgtg
      841 aacgtgatct gatagccctc gccggtgacg aggcccgagc cgttttcgcc gagcgtcaga
      901 atcgtgccgg tcgagtcgaa cgcgacgctg ccgaacgacg atagcggtgc gggaggcgtc
      961 gtcgcggtgc cgccgatcgg ctgcaacgtg aactccgcgt agttgatgtt gcccgagatg
     1021 cccgtctgcg acgtcgcgtt caccgcctgc gagaacgtga acgtgatcga acccgtcggc
     1081 ggctggccga gcacctggaa gttgaggatg acgtacggca ggctgctcgg cggcgccgtg
     1141 aagcacgaca agcacggcac ggcgggctga ccgtgatgcc cgatcgcgag gagcgcgccg
     1201 cctgcggccg cgagcagcgc gagcacgttg aacccgctgt gccccggcgg cggcggaagc
     1261 ggcggcatcg acttggtgct cgtgagattg tcgccgacgg ccggctgata gcccgcgacc
     1321 gcgttcgaga agctgagcca cgcttcgtcg tcggtgatgc tcgaaatcgt cgcgtcggcc
     1381 atcagctggc cgtggcgcgt caccgtgaac gtgtcgccga ccgagaagcc ctcttccgta
     1441 cccgtgtcag ccttcgtgcc gtcggtcgtc gagtagatcg tgccggtacc gcgatgaccc
     1501 gcgctgagcg cggcgaggtt gctcgaaagg cctgcagcga gcgcgcccgg attgccggga
     1561 tacggctgcg agaagacttg cgagccgatg aacgccatcg gtgcgacgcg ataaagcgac
     1621 aagcgatagt acacggagcc gcccgacgcc tggatgacgc tgccgagcag caattcgtcg
     1681 gcgccggcct tcgcggcgtc actcagcgcg cccattaacg ggtcatccgt cagcgtcttc
     1741 ggatagccga gcggtccgcc gcc
//
LOCUS       DASUCJ010000899         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1476684, whole genome shotgun sequence.
ACCESSION   DASUCJ010000899 DASUCJ010000000
VERSION     DASUCJ010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476684"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..589
                     /locus_tag="VFU90_10715"
     CDS             164..589
                     /locus_tag="VFU90_10715"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5480297.1"
                     /translation="MKRSASAIWQGGLKTGKGSISTESGVLANAPYSFGTRFENAKGT
                     NPEELIGAAHAGCFAMALSLVLGEAGLTPERLETSAAVTIDKVADGFAITSVHLTLRA
                     KIPGASREAFERAAEAAKAGCPVSKVLNAKITLDKTLDA"
     gene            661..936
                     /locus_tag="VFU90_10720"
     CDS             661..936
                     /locus_tag="VFU90_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480298.1"
                     /translation="MEPFAVLVLLPTLVGIASELVFRDALHASLAAALVSALAVYTCL
                     DLLDPGGTWNALAGFLVSPLVMAVSLVAVLTCFGHLAGRRTLRRQRN"
     gene            967..1959
                     /locus_tag="VFU90_10725"
     CDS             967..1959
                     /locus_tag="VFU90_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5480299.1"
                     /translation="MRRTPLYSGSQPARNAQSATTPAPEATPASAASPAFAATPAIAA
                     TPAQSGEHKRHPLRAAARGLRRTGTFLLRHEGVVAIAAAGIVAAAIAWSYAASHPAPP
                     PLTQDDFDHAVMHTVEHNVLPSQAARAYEAIRGSVVRVMDFETTEDEGDVETGVGSGV
                     VIVNDGTILTNLHVVAGAKRVGVVFADGTESDADLIGTQPQNDLAVLKARTIPDDLAA
                     ATLRSTADLAPGDDVIAVGFPFGIGPSASAGVISGLKRQFRSPEGKRLLTNLIQFDAA
                     ANPGNSGGPLVTMDGAVVGIVTGILNPTSQRVFVGIGFAVPIESAAAAVGMSPF"
     gene            2003..>2221
                     /locus_tag="VFU90_10730"
     CDS             2003..>2221
                     /locus_tag="VFU90_10730"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5480300.1"
                     /translation="MDHIGTAHVASQMERVLYEVKKVVVGQDRFLERVLVAILAQGHL
                     LVEGVPGLAKTLTVKTLARVVHGTFRRIQ"
BASE COUNT          365 a          766 c          736 g          354 t
ORIGIN      
        1 agagctcatc gtatcgctcc ggttggtcgt tcgaagcaaa tccggccggt cgttcgcagc
       61 aagacgtcca tcgccgaaca gccgtcaaca gtcgccgcgc atcccggatt cccggaaacg
      121 cagtgctacc atcgagcgat caggcatcgt ccgggagaac gcaatgaagc gcagcgcatc
      181 cgcaatctgg cagggtggac tcaagaccgg caagggtagc atctccaccg aaagtggagt
      241 gcttgcgaac gcaccgtatt cgttcggcac gcgcttcgag aacgcgaagg gcacgaatcc
      301 cgaggagctg atcggcgcgg cgcacgccgg ctgcttcgcg atggcgcttt cgctcgtgct
      361 cggcgaggcg ggcctcacgc ccgagcgtct ggagacatcg gccgcggtga cgatcgacaa
      421 ggtcgccgac ggcttcgcga tcacctcggt acacctcacg ctgcgcgcga agattcccgg
      481 cgcatcgcgc gaagccttcg agcgcgccgc cgaagcggcg aaggcgggat gcccggtgtc
      541 gaaagtgctc aacgcgaaga tcacgctcga caagacgctc gacgcctgac aggagtacga
      601 tgaccgggcc cgtcgcgcgc gacgcgcggc ggagggaagt tgctcccgtg cgggtgcccg
      661 atggagcctt tcgcggtcct ggtgctgttg ccgacgctcg tcggaatcgc ttcggagctc
      721 gtgttccgcg atgccctgca tgcgtcgctc gcggctgcac ttgtgtccgc gcttgcggtc
      781 tatacctgcc ttgacctgct cgaccccggt ggaacctgga atgcgctggc gggcttcctg
      841 gtttcaccat tggtgatggc ggtctcgctc gttgccgtgc tgacgtgctt tggtcatctc
      901 gcgggacggc gcacgctgcg cagacagcgc aattgacgat ccggccggcg tgaccggccc
      961 ctggcgatgc gccgaacccc actctacagc ggctcccagc ccgcgcgaaa cgcgcaaagc
     1021 gcgacgacgc cggcgcccga ggcgacgccc gcatccgcag ccagccccgc attcgcagcc
     1081 acgcctgcaa tcgcagccac ccctgcgcaa tcgggcgagc acaaacgcca cccgctgcgt
     1141 gcggcggcgc gcggcctgcg gcgcaccggg acattcctgc ttcgtcacga aggggtggtg
     1201 gcaatcgcag cagccgggat cgtcgccgcg gcaatcgcgt ggagctatgc ggcgtcgcac
     1261 cccgcgccac ctccactcac ccaggacgac ttcgatcacg cggtcatgca cacggtcgag
     1321 cacaacgtat tgccgtcgca ggccgcgcgt gcctacgagg cgatccgcgg gtcggtcgtg
     1381 cgcgtgatgg acttcgagac gacggaggac gagggcgacg tcgagaccgg cgtcggcagc
     1441 ggggtcgtga tcgtcaacga cggcacgatt ctcaccaatc tccacgtcgt cgccggggcg
     1501 aagcgcgtcg gcgtcgtgtt cgccgacgga acggaatcgg acgccgacct gatcggcacg
     1561 cagccacaga acgacctcgc ggtgctgaag gcgaggacca ttcccgacga cctcgctgcc
     1621 gcgacgctgc gctcgaccgc cgacctcgct cccggcgacg acgtgatcgc ggtcggcttt
     1681 ccgttcggga tcgggccgtc ggcgagcgcg ggcgtgatct ccggactcaa gcgacagttc
     1741 cgttctccgg agggtaagcg cttgctcacc aatctgatcc agttcgatgc cgcggcgaac
     1801 cccggcaact cgggtggccc gctggtgacg atggacggcg cggtcgtcgg cattgtcacc
     1861 ggcatcctca acccgacgtc gcagcgggta ttcgtcggca tcggcttcgc ggttccgatc
     1921 gaaagcgcgg cggcggcggt cggcatgtcg ccgttctgag tcgcttcccg cacgcttcga
     1981 cccacccttc gacaggcatc ccatggacca catcggcacc gcccacgtcg catcgcagat
     2041 ggagcgcgtt ctctacgagg tcaagaaagt cgtcgtcggg caggaccgct tcctcgagcg
     2101 cgtgctggtc gcgatcctcg cgcaggggca cctgctggtc gaaggcgttc ccggactcgc
     2161 gaagacgctc accgtgaaga cgctggcgcg cgtcgtccac ggcacgttcc ggcgcatcca
     2221 g
//
LOCUS       DASUCJ010000900         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_957003, whole genome shotgun sequence.
ACCESSION   DASUCJ010000900 DASUCJ010000000
VERSION     DASUCJ010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957003"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFU90_10735"
     CDS             <1..853
                     /locus_tag="VFU90_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480301.1"
                     /translation="RRLIGLLSASVLALGSALVPTPVDAEMAGGGGNVDPASGTVSVH
                     IHTESGGDDVVVDLSGHSRTQLVDFSIVRVPAQNGSEDGLCLAGRKPDGTPQLGFNYH
                     QVGRSHIDGSVVYDEYVCVPFTNPGDTPSPPPSPQLPTIEDAWRAANLPTPVINLDPA
                     TRGITGLDTRLWTDAPQTIRISAEAGGYTITGNATVTGYELAIDDQPPITVGDPGTND
                     RPIAHYTFETRGLHHIRVGIVWHGDAQMTGPDLLRPIGVMSIGDATITATRDYQVNEI
                     RSVLQPG"
BASE COUNT          186 a          341 c          341 g          170 t
ORIGIN      
        1 gagacggctc atcggtctgt tgtccgcgag cgttctcgcg ttgggatcgg cacttgttcc
       61 gacgccggtc gatgcggaga tggccggcgg cggtggcaat gtcgatcccg cgagtgggac
      121 cgtctcggtc cacatccaca ccgagagcgg cggagacgat gttgtcgtcg atctgtccgg
      181 tcattcgcgc acgcagttgg ttgatttcag catcgtgcgc gtgcccgcgc agaacggcag
      241 cgaggacggg ctttgcctgg ccggccggaa gccggacggc acgccgcagc tcggcttcaa
      301 ctatcaccaa gtcggacggt cacatatcga cggcagtgtt gtgtacgacg aatatgtgtg
      361 cgtgccgttc acgaaccccg gcgacacgcc gtcgccacca ccgtcgccgc agctgcccac
      421 catcgaagat gcgtggcgcg ctgcgaacct gccgacgcct gtcatcaatc tcgatccggc
      481 gacgcgcggg atcaccggtc tcgacacgcg actgtggacc gacgcgccgc aaacgattcg
      541 catctcggcg gaagcgggcg gttacacgat taccggaaac gcgaccgtga ccggttacga
      601 gctcgcgatc gatgatcagc cgccgatcac cgtcggtgat ccggggacga acgatcgccc
      661 gatcgcgcac tacacattcg aaacgagggg cctgcatcac atccgtgtcg gaatcgtctg
      721 gcacggagac gcgcagatga cgggcccgga tcttctgcgg ccgatcggag tgatgagcat
      781 cggcgacgcg acgatcaccg cgacacgcga ctaccaggtg aacgagatca ggtcggtgct
      841 ccaacccgga tgatcgtcaa cggtcaatcc gggagaagcg tcgtgccggc gtcacccgcg
      901 agcaccgctt cgggttcgtc gagcgcgccg atcgcggcgc gacggccggt cgtcgccacg
      961 aaccaggctg ccgcgtcgac cttcggtccc atcgatcccg gcgcgaacgt gtgccggcgc
     1021 agttccgaca cggtgacc
//
LOCUS       DASUCJ010000901         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1079475, whole genome shotgun sequence.
ACCESSION   DASUCJ010000901 DASUCJ010000000
VERSION     DASUCJ010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079475"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..>1057)
                     /locus_tag="VFU90_10740"
     CDS             complement(27..>1057)
                     /locus_tag="VFU90_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU5480302.1"
                     /translation="LVCRFMDVDAFVTAHRASWARLQQLVRRAQRPSQLSGTEIDELV
                     SLYQRTATHLSLVQSRSPDAHLIADLSRLTAAARGVITGSRDPSFSDVARYLKITLPA
                     ALYRSARWWVPIAFANIAVMFALGIWMGTHPHVITSLVPSDAVRQLVEHDFKNYYSAH
                     PATDFAARVWTNNALVAAGSLALGVFLGLPTLYLLWVNCVNVGVSGGILAANGKLGEF
                     FALILPHGMLELTAVFIAAGTGLRIGWTIIDPGPRKRADALAAEARPAFAVAIGLVGV
                     LLVSGVIEAFVTPSPLPTWARISIRGIVEVAFLTYVFGLGRRAARAGETGDVEEWLRA
                     DAAVFSAD"
BASE COUNT          202 a          349 c          363 g          143 t
ORIGIN      
        1 ggtcaagcgc cggaaagact ggcgggttag tcggcggaaa agaccgccgc gtcggcgcgc
       61 agccactctt cgacgtcacc ggtctcgcca gcgcgcgcag cgcgtcgtcc gagcccgaaa
      121 acgtaggtca ggaaagcgac ctcgacgatc ccgcggatcg agatgcgcgc ccacgtcggc
      181 agcggcgacg gcgtcacgaa cgcctcgatc acgcccgaca ccagcagcac ccccaccagg
      241 ccaatcgcca cggcaaacgc tggccgggct tcggccgcaa gcgcgtcggc gcgtttgcgt
      301 gggccggggt cgatgatggt ccagccgatc cgcagtccgg tgccggccgc gatgaacacc
      361 gcggtgagct cgagcatgcc gtgcggcagg atcagcgcga agaactcgcc aagttttcca
      421 ttcgcagcga ggattccgcc ggacaccccg acgttgacgc agttcaccca cagcaggtaa
      481 agcgtgggca aaccgaggaa aacgccgagc gccaagctgc ccgcggcgac caaagcgttg
      541 ttggtccaca cccgggcggc gaagtcggta gccggatgcg ccgagtagta gttcttgaaa
      601 tcgtgctcga caagttggcg gacggcgtcc gacggcacca ggctggtgat gacgtgcggg
      661 tgcgtgccca tccagattcc gagcgcgaac atcaccgcga tgttcgcgaa cgcaatcggc
      721 acccaccatc gggcgcttcg gtacaacgcg gccggaaggg tgatcttcag gtagcgcgcg
      781 acgtcggaga acgacggatc gcgcgacccc gtgatgacgc cgcgcgccgc ggccgtcaat
      841 cgcgacaggt cggcgatcag gtgcgcgtcg ggtgatcgcg actgcacgag cgagagatgg
      901 gtggcggttc gctggtacag cgacacgagt tcgtcgatct ccgtgccgct cagctgggac
      961 ggacgctgcg cccgccgcac cagctgctga agacgcgccc agctggcgcg atgcgcggtc
     1021 acgaacgcgt cgacgtccat gaaccggcag actagtg
//
LOCUS       DASUCJ010000902         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_330203, whole genome shotgun sequence.
ACCESSION   DASUCJ010000902 DASUCJ010000000
VERSION     DASUCJ010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330203"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFU90_10745"
     CDS             <1..816
                     /locus_tag="VFU90_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5480303.1"
                     /translation="YYHDVAGHGTFVSSLIATNSLGIAAVAPQTDLVMVRVLDDNGAG
                     DDFAVISGIIYAADNGADVINVSLGGYLSRSNAFDLAISDLIQRAIDYAVARGALVVA
                     AAGNESLNSNTATSPKGSYSDSLHVLAGGIRQVISVGATGPVDQKNFDSIAVYSNFGK
                     VDVAVFAPGGNTVDSTNFSDLVIGACSQFVFVECANERAYLSGAGTSFSSPLVAAEAA
                     VIKAHARSAPSPTQLESCILNSADAPKGKSKPDVNYNFGRIDVVNGALRSKCA"
     gene            complement(859..1371)
                     /locus_tag="VFU90_10750"
     CDS             complement(859..1371)
                     /locus_tag="VFU90_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5480304.1"
                     /translation="MAAAIVPGLVAAQSSSSPVADAFRENASRTGKNLIAAAETMPAD
                     KYSFKPTPAQMSFGQIVVHLSQGNDYLCGTIGGEKAPTRTKVADTASKEVLIARLKET
                     FAFCDQALAKLDDSKLSEQLPFFGGKTRSRAAIMTLTTGDWADHYSQSAIYLRLNGEL
                     PPTAKGKQAE"
     gene            complement(1662..>3272)
                     /locus_tag="VFU90_10755"
     CDS             complement(1662..>3272)
                     /locus_tag="VFU90_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5480305.1"
                     /translation="CQSTSGLHFELAFPNATGPRTGRAYVAVARDGTPEPRLQAGELA
                     RSTPFFGTDVRALPPGHTVIVDAHAAGYPLRSLASLPAGDYWVQAVFNVYTEFHRADG
                     RTLWLHQDQWEGQKWNESPGNLVSEPQRIHLDPHRTQTVRLTLSRTLPPIALPPDTKW
                     VKHVKIQSRLLTAFWGHPMYLGATVLLPAAYDEHADQHYPVIYEQGHFTLSPPFGFDP
                     DGTPETAEHAARRRMNTEREPGYEFARAWMSDSFPRVIAITFQHPTPYYDDSYAINSV
                     NDGPYGDAIVQELIPYLEKQFRIIAKPYARILVGGSTGGWEALALQIYHPDLFGGAWG
                     LYPDPVDFRRYQLSNIYDDTSAFVVARSPWLASEVPAERETSGMPTITMRQESQLEDA
                     LGTHGRSGEQLEAWEAVWGPTDADGYPAALWDKRTGHMNHQVAAYMRDHGYDLRAYLQ
                     NHWSTVGPQLQGKLHVVVGEMDSYYLNLACYLLQDFLDSTTAPHSDATFTYGRPLKPH
                     GWQPWTDAAFVRMAFDTISAHASADAGR"
BASE COUNT          541 a         1092 c         1057 g          582 t
ORIGIN      
        1 tactaccacg acgtcgccgg acacggcacg ttcgtctcgt ctctcattgc caccaattcg
       61 ttaggcatcg ccgccgtcgc cccgcagacg gacctggtca tggtccgcgt gctcgacgac
      121 aacggcgccg gcgatgactt cgccgtcatc agcggcatca tctacgccgc cgacaacggc
      181 gccgacgtca tcaacgtcag cctcggcgga tacctcagcc gctccaatgc gttcgacctc
      241 gccatctcgg atctcatcca gcgcgccatc gactacgccg tcgcacgcgg cgcgctggtc
      301 gtcgccgccg ccggcaacga gtcgctcaac tccaacacgg ccacgagccc caaaggcagc
      361 tatagcgaca gcctgcacgt gctcgccggc ggtatccggc aagtgatcag cgtgggcgcc
      421 acgggtccgg tcgatcagaa aaacttcgac tcgatcgccg tgtactcgaa tttcgggaag
      481 gtcgacgtgg cggtgttcgc gccgggcggc aacaccgtcg actcgacgaa tttcagcgat
      541 ctcgtgatcg gcgcctgcag ccagttcgtc tttgtcgagt gcgcgaacga acgcgcctac
      601 ctctcgggcg ccggcacgag cttctcgtct cccctcgtgg cggcagaagc cgcggtcatc
      661 aaggcgcacg cgaggagcgc accgagtccg acccagctcg agtcgtgcat cctcaattcg
      721 gccgatgccc cgaaggggaa atcgaagccg gacgtcaact acaacttcgg acgaatcgac
      781 gtcgtcaacg gagcgctccg ctcgaagtgc gcgtaaggcg tccgcgacga catcgatccg
      841 cccgatcgat ggaccacact actccgcttg cttgcccttc gcggtcggcg ggagttcgcc
      901 gttgagccgc agatagattg ccgactggct gtagtggtcg gcccagtcgc cggtggtcag
      961 cgtcatgatc gcggcacgcg accgcgtctt cccgccgaag aacggcagct gctcactcag
     1021 cttcgagtca tccagcttcg ccagcgcctg gtcgcagaag gcgaaggtct ccttgaggcg
     1081 cgcaatgagc acctccttgc tggcggtatc cgccaccttg gtgcgcgtcg gagccttctc
     1141 gccgccgatc gtgccgcaca gataatcgtt gccttgggac agatgcacga cgatctgacc
     1201 gaagctcatc tgcgcggggg tcggcttgaa gctgtacttg tcggccggca tcgtctcggc
     1261 ggcggcaatc aggttcttgc cggtacggct ggcattttcg cggaatgcgt cggccaccgg
     1321 gctcgacgac gactgcgccg ccacgagtcc cggcacgatg gcggcagcca cagtcgcaag
     1381 acaaatacgg acacgcatgg atctcgctcg tgtttgggga gaggttgatg ttggttgaca
     1441 tcgcgaacga tgtccagacc gggtcgtggc tccggtcctc gccggcacgc ggcgcctgag
     1501 cgtccatcgt cccgccgccc ctggggtccc accggagcgt catgatcacg cgcgccgatc
     1561 cgtcgtcaaa agagcggatt ctcgtcgccg cgcctcctga tcgaattcct gaatgatcgc
     1621 atccgccaac gccatacgga ctccacggtc gatgtttttg cctaacggcc ggcgtccgcc
     1681 gaggcgtggg ccgagatcgt gtcgaaggcc atgcgcacga acgccgcgtc cgtccacggc
     1741 tgccacccgt gcggcttgag cggccggccg tacgtgaacg tcgcgtccga gtggggcgcc
     1801 gtcgtgctgt cgagaaaatc ctggagcaga tagcaggcca ggttgagata gtagctgtcc
     1861 atctcgccga ccacgacgtg gagtttcccc tgcagctgcg ggcccaccgt actccagtga
     1921 ttctggaggt acgcgcgcag gtcgtagccg tggtcgcgca tgtacgcggc cacctggtga
     1981 ttcatgtgcc cggtgcgctt gtcccacaag gcggcggggt agccgtcggc gtccgtgggt
     2041 ccccacaccg cttcccacgc ctcgagctgc tccccggagc gcccgtgcgt gcctaacgca
     2101 tcctcgagct ggctctcctg gcgcatcgtg atcgtcggca tgccgctggt ctcgcgctcc
     2161 gccggcacct ctgaagcgag ccacggactg cgggccacga cgaacgcgct cgtgtcgtcg
     2221 tagatgttgc tcaactgata ccgccggaaa tccactgggt cgggatagag accccaggcg
     2281 ccgccgaaca agtccggatg atagatctgc agcgccagcg cttcccaccc acccgtggat
     2341 ccgccgacca ggatccgcgc atacggtttg gcgatgatgc ggaattgctt ctcgagatac
     2401 gggatgagct cctgcacgat cgcgtcgccg tagggcccgt cgttcacgga attgatggca
     2461 tacgaatcat cgtagtacgg cgtcggatgc tggaacgtga tcgcaatgac gcgcggaaaa
     2521 ctgtcgctca tccaggcgcg cgcgaattcg taaccgggct ctcgctcggt gttcatgcgc
     2581 cgccgcgccg cgtgctccgc tgtttcgggc gtgccgtcgg gatcgaaacc aaagggcggc
     2641 gacaacgtga agtgtccctg ctcgtaaatc accggatagt gctgatcggc gtgctcgtcg
     2701 tatgcggccg gcagcagcac ggtggcgccc aggtacatcg gatggcccca gaaggcggtg
     2761 agcaggcggc tctggatttt gacgtgcttc acccacttgg tgtcgggcgg cagcgcgatc
     2821 ggcggcagcg tgcgcgagag cgttaggcga acggtctgcg tgcggtgcgg atccaggtgg
     2881 atgcgctgcg gctcgctcac cagattgccc ggcgattcgt tccacttctg gccttcccac
     2941 tggtcctggt gcagccagag ggtgcgtcca tcggcgcggt ggaattccgt gtacacgttg
     3001 aacaccgcct gcacccaata gtcgcccgcc ggcagcgacg cgaggctgcg caacggatag
     3061 cccgccgcgt gcgcgtcgac gatgaccgtg tggcccggcg gtagcgcgcg cacgtcggtg
     3121 ccgaagaacg gcgtgctgcg agcgagctcg ccggcttgca gtcgcggctc cggcgtgccg
     3181 tcccgggcaa cggcgacgta cgcgcgcccg gtgcgcggcc cggtcgcgtt cggaaaggcg
     3241 agctcgaagt gcagcccgga cgtggattgg ca
//
LOCUS       DASUCJ010000903         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_666765, whole genome shotgun sequence.
ACCESSION   DASUCJ010000903 DASUCJ010000000
VERSION     DASUCJ010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666765"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..900)
                     /locus_tag="VFU90_10760"
     CDS             complement(22..900)
                     /locus_tag="VFU90_10760"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5480306.1"
                     /translation="MTPNGQSSRHEPYTTVVAPSVVREHLEDPAYLVVDCRHSLADFS
                     LGKRLYDEAHVPGAFFADVEYDLAGPKTGANGRHPMPDPEAFARFLRARGAGDATQIV
                     AYDAGADMFAARLWFLCRWIGHHAVAVLDGGFAAWVAAGYPVTAAPEQPQREGTLEVR
                     LHPELVVDADYVLQRLRDPGMQLLDARAEDRFAGQNETVDPVAGHIPGALNRWFKRNF
                     NGDGSFKSAAALRDEFAAQGLDPKRVVHQCGSGVSSAANELAMAIAGLEGSRLYAGSW
                     SEWIADPARPIVTGTE"
     gene            complement(920..1279)
                     /locus_tag="VFU90_10765"
     CDS             complement(920..1279)
                     /locus_tag="VFU90_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEU5480307.1"
                     /translation="MSEQLKEEIAREIAANTVLVYGKGTKSQPRCGFTLETIQFFDNF
                     GVPFEVVDVLENVPKREALSEMTQWPTLPKVFINGTFYGDTDILGPMAESGELQEVLK
                     AAFGDAAPAPKHTIRLH"
     gene            complement(1281..1523)
                     /locus_tag="VFU90_10770"
     CDS             complement(1281..1523)
                     /locus_tag="VFU90_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEU5480308.1"
                     /translation="MIDDQGLTGLIRARIPDAEVQVFDRTGTMDHFNVTVRSEAFNGK
                     TLIEQHQLVYGALKAALKDGRVHAVELRTIAVPEKR"
     gene            complement(1520..>1759)
                     /locus_tag="VFU90_10775"
     CDS             complement(1520..>1759)
                     /locus_tag="VFU90_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480309.1"
                     /translation="NTVKAVLRGVARLQSTFRIEIGPLRASGVPAILIGVGGIVLASG
                     IAAALSRGANRLPETLGEARGLAEAMNARSLRLKS"
BASE COUNT          280 a          585 c          578 g          316 t
ORIGIN      
        1 gcgctaaggg cgagctagcc gttattccgt tccggtaacg atcggacgcg cgggatcggc
       61 gatccactcg ctccacgaac cggcgtacag gcgcgaccct tcgagcccgg cgatggccat
      121 tgcgagttcg ttggctgccg aggaaacgcc cgacccgcac tggtgcacga cgcgtttggg
      181 gtcgagcccc tgcgccgcga actcgtcgcg taaggcggcg gccgatttga acgagccgtc
      241 gccgttgaag ttgcgcttga accagcgatt gagcgcgccc ggaatgtgcc ccgcgaccgg
      301 atcgacggtt tcgttttgtc cggcgaaacg gtcttcggcg cgcgcgtcga gcagctgcat
      361 ccccgggtcg cgcaggcgtt gcagcacgta gtccgcgtcg accaccagtt cggggtgcag
      421 ccgtacctcg agcgttccct cgcgctgcgg ctgctcgggc gccgccgtca ccgggtatcc
      481 cgcggcgacc caggcggcga atcctccgtc gagcacggcg accgcgtggt ggccgatcca
      541 ccggcaaaga aaccaaaggc gcgccgcaaa catgtccgcg cccgcgtcgt aggccacgat
      601 ctgcgtcgcg tcgccggcgc cgcgcgcgcg caagaaacgc gcgaacgctt cgggatcggg
      661 catcgggtgc cgtccgttcg cgccggtctt cgggccggcg agatcgtact cgacgtcggc
      721 gaaaaacgcg cccggcacgt gcgcctcgtc gtacagccgc ttgcccagcg aaaaatcggc
      781 tagcgaatgc cggcaatcga cgacgaggta ggcggggtcc tcgaggtgct cccgcacgac
      841 cgacggcgcg acgacggtgg tgtaaggctc gtgccggctc gattgcccgt tcggagtcac
      901 gcgaaactcc tccagttcgc tagtgaaggc gaatggtgtg cttcggcgcc ggcgccgcgt
      961 cgccgaaagc cgctttgagc acttcctgca gctcgccgct ttcggccatc gggccgagga
     1021 tgtcggtgtc gccgtaaaag gttccgttga tgaacacctt cggcagcgtc ggccactgcg
     1081 tcatttcgct cagcgcttcg cgcttgggca cgttctccag cacgtcgacg acctcgaacg
     1141 gtacgccgaa gttgtcgaag aactggatcg tctcgagggt gaaaccgcag cgcggctgcg
     1201 atttggtgcc tttaccgtag accaagaccg tgtttgcggc gatctcgcgc gcgatttctt
     1261 ctttgagctg ttccgacatg ttagcgtttc tccgggacgg cgatcgttct gagctcgacc
     1321 gcgtgcaccc gtccgtcctt gagcgccgct ttgagcgcgc cgtagaccag ctggtgctgt
     1381 tcgatgaggg tcttaccgtt gaacgcttcg gagcgcacgg taacgttgaa atgatccatc
     1441 gtgccggtgc gatcgaacac ctgcacttcc gcgtcgggaa tgcgcgcgcg tatcaggccg
     1501 gtcagaccct ggtcgtcgat cacgatttca ggcgcaagga acgtgcgttc atcgcttccg
     1561 ccaacccgcg cgcctcgccg agggtttcgg gcagccggtt ggcgccgcgc gagagcgccg
     1621 ccgcgatgcc gctcgcgaga acgattccgc ccacgccgat caggatcgcc ggaaccccgc
     1681 tggcgcgcaa cggtccgatc tcgatacgaa acgtcgattg cagtcgcgcg accccgcgca
     1741 gcacggcttt gaccgtatt
//
LOCUS       DASUCJ010000904         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1324008, whole genome shotgun sequence.
ACCESSION   DASUCJ010000904 DASUCJ010000000
VERSION     DASUCJ010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324008"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..>1125
                     /locus_tag="VFU90_10780"
     CDS             123..>1125
                     /locus_tag="VFU90_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5480310.1"
                     /translation="MSRHNLAAGIARYFLDSKLTPALVIAIVTLGLLAIWQTPREENP
                     QITMPAATIVTEYTGNSAAEVDRLVTQRGERVLAEVAGIEHVYTVSSQNLSLITVLFH
                     VGDDPTKSFVDLYDHVNAHLDELPRGASQPRITPLAVDDIPIVVLTLHGKNYNRGELY
                     AAAERLIDAIRPLDGVAAITTYGGLPRAVDVKLDPVRLGAYGLSPVAIARAIAATNVE
                     AAAGALRSDAGEIPLHVGTPFTSARQTASQIVGVSGGAPVTLGEVASVAMGWVPEENQ
                     TRFGYGGAFATEAGATEPAVSIAIAKKPGSNAVAIADAVRARVASTEIPPGVAVTVTR
                     "
BASE COUNT          203 a          381 c          369 g          172 t
ORIGIN      
        1 caaaccggaa cgttcggcaa agaacgcgtc gccgtcgcag gcaacctgcg ttccggcgag
       61 cgggtgatta cgtccccgct ggaacgcgtg accgacggcg cggccgtgac gccaagcgcg
      121 ccatgagccg gcacaaccta gcggccggga tcgcccgcta ttttctcgac tcgaagctga
      181 cgccggccct cgtcatcgcc atcgtgacgt tgggtttgct ggcgatttgg cagacgccgc
      241 gcgaagagaa tccgcagatc acgatgccgg cggcgacgat cgtcaccgag tataccggca
      301 attccgccgc cgaagtcgat cgcttggtga cgcaacgcgg cgaacgcgtc ctggccgagg
      361 tcgccggtat cgaacacgtc tacaccgtgt cgtcacagaa tctctcgctc ataacggtac
      421 tctttcacgt cggcgacgat ccgaccaaat cgttcgtcga tctgtacgac cacgtcaacg
      481 cgcacctcga cgagctgccc cggggcgcct cgcagccccg aatcaccccg ctggcggtcg
      541 acgatattcc gatcgtcgta ctgacgctgc acgggaagaa ctataatcgc ggcgagcttt
      601 acgccgctgc cgaacggctg atcgacgcga ttcgcccgct cgacggtgtg gccgcgatca
      661 cgacctacgg cgggctgccg cgcgcggtcg acgtaaaact cgacccggtc cgtctaggcg
      721 cgtacggtct gtcgccggtc gcgatcgcgc gcgcgatcgc agcgacgaac gtggaagccg
      781 ccgcgggcgc gctgcgcagc gacgccggcg aaatcccgct gcacgtcggc acacccttta
      841 cctcggcacg gcaaaccgcg tcgcagatcg tcggcgtcag cggcggcgca cccgttacac
      901 tgggcgaggt cgcctcggtc gcgatgggat gggtgccgga agaaaaccag acgcgtttcg
      961 ggtacggcgg ggcgtttgcg acggaagccg gcgccaccga accggcggtc agcattgcga
     1021 tcgccaaaaa gcccggcagc aacgccgttg cgatagcgga tgcggtgcgc gcgcgcgtcg
     1081 catcgacgga aataccgccg ggcgttgcgg taacggtgac gcgcg
//
LOCUS       DASUCJ010000905         1396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1293501, whole genome shotgun sequence.
ACCESSION   DASUCJ010000905 DASUCJ010000000
VERSION     DASUCJ010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293501"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFU90_10785"
     CDS             complement(<1..1154)
                     /locus_tag="VFU90_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5480311.1"
                     /translation="MRLTDALMRRAEEALTFDPRQQRRVLLDRTAVAAADVSTLRERI
                     VQALGEGADAAQLAIDRLVGTTDQLNVSYLERGMIAADAVARIEVRDGDSAVVGSATG
                     FMISPRLMLTNHHVLPTADDAAQSWLHFRHEFDALGRALEPCVFALEPSTFFWSSQHL
                     DATVVAVAPETPDRRQRVARFGWLRLSASETLVLPGEWLTTIHHPGGAPKQLALRENL
                     LIKRGSCDLWYATETASASSGAPLFNDSWQVVGMHRVGVPVRDANGGVLMLDGQRWTE
                     DADESRVVWRAGVGTCTSAIVRQLASEARDHPVVVELLAESDADAADAIVVPLGAGAD
                     RAGASGAANGETDRAAPTAAAVSMPLAAESNGGSGHDHSVLDGDALTVTVP"
     gene            complement(1183..>1396)
                     /locus_tag="VFU90_10790"
     CDS             complement(1183..>1396)
                     /locus_tag="VFU90_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480312.1"
                     /translation="MFLWLPVPSGFTSQRWTEWLIEHAGVVVTPGNAFGPGGEGYFRV
                     SLVAAGSVLDEAIGRLRSAGVRYARR"
BASE COUNT          237 a          492 c          460 g          207 t
ORIGIN      
        1 ggcaccgtca cggtgagcgc atcgccgtcg aggacggaat ggtcatggcc cgaaccgccg
       61 ttcgattcgg cagccagcgg catgctcaca gcggcggctg tcggggcggc ccgatcggtt
      121 tcgccgttcg ccgctcccga cgcgccagcc cggtcggcgc ctgcacctaa cggcacgacg
      181 atcgcgtccg ccgcgtcggc gtcgctctcg gcgaggagct cgacgacgac agggtgatca
      241 cgagcttccg acgcgagctg ccggacgatc gcggatgtgc acgtcccgac accagcgcgc
      301 cagacgaccc ggctctcgtc cgcatcctcg gtccatcgct ggccatcgag catcaggaca
      361 ccaccgttgg catcacgcac cggcacgccg acccggtgca tcccgaccac ctgccatgag
      421 tcgttgaaca gcggggcgcc cgaagacgcg gaagccgttt ccgtcgcgta ccacaggtcg
      481 cacgacccgc gtttgatgag taaattctca cggagtgcga gctgtttcgg cgcgccgccg
      541 gggtgatgga tggtcgtgag ccactctcca ggcagtacga gtgtctcgct cgcggacaac
      601 cgcagccatc cgaatcgcgc gacgcgctgc cggcgatccg gcgtctccgg ggccaccgct
      661 acgaccgtcg cgtcgaggtg ctggctgctc cagaaaaagg tggacggctc gagtgcgaag
      721 acgcatggct cgagcgcgcg acccagcgcg tcgaactcgt gccgaaagtg tagccaactc
      781 tgcgccgcat cgtcggccgt cggcagcacg tggtgattgg tgagcatcag ccgcggcgag
      841 atcatgaaac cggtggctga gccgacgacc gcgctatctc cgtcgcgtac ttcgatgcgc
      901 gcgacggcat cggccgcgat catgccacgc tcgagatagc tcacgttgag ctgatccgtg
      961 gtgccaacca gacggtcgat cgcaagctgc gcggcgtccg cgccctcgcc cagcgcctga
     1021 acgatccgtt cgcggagcgt cgacacgtca gccgccgcga ccgcggttcg atccaggagg
     1081 acccgccgct gctggcgagg gtcgaaggtc agcgcctctt cagcgcggcg catcagcgcg
     1141 tcggtgagcc tcatccgggc gaatctcgcg ggcctgtcgc gctcagcggc gagcgtagcg
     1201 gacaccggcg ctacggaggc gtccgatcgc ttcatccagc acgctcccgg ccgcgacgag
     1261 tgaaacgcgg aaatagcctt cgcccccggg accgaacgcg tttcccggcg taaccacgac
     1321 gcccgcatgc tcgatgagcc attccgtcca gcgctgcgac gtaaacccgg atggaacggg
     1381 cagccagagg aacatc
//
LOCUS       DASUCJ010000906         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_850028, whole genome shotgun sequence.
ACCESSION   DASUCJ010000906 DASUCJ010000000
VERSION     DASUCJ010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850028"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFU90_10795"
     CDS             <1..620
                     /locus_tag="VFU90_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5480313.1"
                     /translation="GRPVITATQMLESMVSNPRPTRAEASDVANAILDGTDAVMLSAE
                     TAMGEYPQLAVHAMTRIILEIERRPIPQAAKVLARRDSDAPVSTEDAIAAATTAAANS
                     LDAPVVMVFTKSGFSARIVSAHRPGVPIYAITDVERTYRQLALVWGVYPELVSTAATY
                     EEMFERGSRAVKDAGFAKAGDRVLVTAGVPFDVPGTTNLLRVEHV"
     gene            617..1399
                     /locus_tag="VFU90_10800"
     CDS             617..1399
                     /locus_tag="VFU90_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5480314.1"
                     /translation="MKLTFLGTGTSFGVPQIGCNCAVCRSPDPRDRRTRTSAVIETDA
                     GTRLLIDTAPELRLQLIANDIDRVDAVLYTHEHADHTHGIDDLRAVSVRRGAPLPLYG
                     APRTLHQLAQRFPYIFDDQVRPAPGTSKPEGHAHPLREGEVVQIGDADVLPIAVPHGD
                     VSVLAYRIGPIAYVTDAKRIPPDAMDAFRGVEVLVLNALLRTSHPTHLSFAEAIDTAR
                     AIGAARTYLTHLTHHNRHADLEAELPPGIAPAFDGLTVRVGG"
     gene            1541..>2240
                     /locus_tag="VFU90_10805"
     CDS             1541..>2240
                     /locus_tag="VFU90_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480315.1"
                     /translation="MTVVNESVLQTDAMDELVREAVFGQGQTKDHAQWLLWEIGQAAG
                     VRPSSIHDLYLARGRGKTHGFTVPAINVRGMAYDTARAVFRTAVNQKAGAFIVEIARS
                     EIAYTDQRPREYLAVMLAAALREGFRGPLFVQGDHFQVNAKKFAVDRRGEVAAVKQLA
                     QEAIAAGFFNIDVDTSTLVDVSLATLDEQQRLNYQTAVDIARYIREIEPDGVTISVGG
                     EIGEVGAHNSTVPEL"
BASE COUNT          384 a          737 c          752 g          367 t
ORIGIN      
        1 tcggccgtcc cgtgattacg gcgacgcaga tgctcgaatc gatggtctcg aacccgcgcc
       61 caacgcgcgc cgaagcgagc gacgtggcga acgcgattct cgacggtacg gatgcggtga
      121 tgttgtcggc cgagacggcg atgggcgagt acccgcagct cgccgtgcac gcgatgacgc
      181 gcatcatcct cgagatcgag cggcgcccga ttccgcaggc ggcgaaagtg ctcgcgcgcc
      241 gagattcgga tgcgccggtg tccacggaag acgcgattgc cgcggcgacg accgctgccg
      301 cgaactcgct cgatgcgccc gtcgtgatgg tgttcaccaa gagcggtttc agtgcgcgca
      361 tcgtgtccgc ccatcggccg ggcgtgccga tctacgcgat cacggatgtc gagcgcacgt
      421 accggcagct cgccctggtg tggggcgtgt atcctgagct cgtgtcgacg gcggcaacgt
      481 acgaagagat gttcgagcgc ggcagccgtg ccgtaaagga cgccgggttc gcgaaagcgg
      541 gggatcgcgt tctggtcacc gcgggcgtcc cgttcgacgt gcccgggacc acgaacctgc
      601 tccgcgtgga acacgtgtga agctcacatt cctgggtacc gggacgagct tcggcgttcc
      661 gcagatcggg tgcaactgcg cggtgtgccg gtcgcccgat ccgcgcgacc gccgcacgcg
      721 caccagcgcg gtgatcgaaa ccgatgccgg tacgcgtctg ctcatcgaca cggcgccgga
      781 gctgcgcttg cagctcatcg ccaacgacat cgaccgcgtc gacgcggtgc tgtacacgca
      841 cgaacacgca gaccacacac acgggatcga cgacctgcgc gcggtatccg tgcgtcgcgg
      901 cgcgccgctg ccgctctacg gcgcgccgcg cacgctgcat cagctcgcac aacggttccc
      961 ctacatcttc gacgaccagg tgcgaccggc gccgggcaca tccaaaccgg aaggacacgc
     1021 gcacccgctg cgcgagggcg aggtcgtgca gatcggcgac gccgatgtgc tgccgattgc
     1081 cgtcccgcac ggcgacgtgt cggtgctcgc gtatcgcatc ggcccgatcg cgtacgtcac
     1141 cgatgcgaag cggattcctc ccgacgcgat ggacgcgttt cgcggcgtcg aggtgctggt
     1201 gctcaacgcg ctcttgcgca cgtcgcatcc cacgcatctc agcttcgccg aagcgatcga
     1261 caccgcgcgc gcgatcggcg cggcgcgcac gtacctcacg cacctcacgc accacaaccg
     1321 ccatgccgat ctcgaggcag aactgccgcc aggaatcgcg ccggcgttcg atggtcttac
     1381 ggtgagggtg ggcggttgat cgcgcccctt gcgggcgctg gctcggtcgg gcagtgttcc
     1441 tttcatgccg cgctaaccgg cggcgtgaca gtgtcgtctt tcgcgcttgg ggggctgggt
     1501 gcatcagttc ggcaatgacg tcacagtcga acacggtcga gtgacggtcg tgaacgagtc
     1561 ggtgctccag accgatgcga tggacgagtt ggttcgcgag gcggtattcg gacagggaca
     1621 aacgaaggat cacgcgcagt ggctgctctg ggagatcggg caggcggccg gtgttcgtcc
     1681 ctcgagcatt cacgatctct atctggcgcg cggccgcggc aagacgcacg gcttcacggt
     1741 gccggcgatc aacgttcgcg ggatggcgta cgacacggcg cgcgcggtgt ttcgcacggc
     1801 agtgaatcag aaggccggcg cattcatcgt cgaaatcgcc cgctctgaga tcgcctacac
     1861 ggaccaacgc ccgcgcgaat atctcgccgt gatgctcgct gccgcgctgc gcgaaggatt
     1921 ccgcggtccg ctgttcgtgc agggcgatca cttccaggtg aacgcaaaga aatttgctgt
     1981 cgaccgtcgc ggcgaagtcg cggccgtgaa gcagctcgcg caggaagcca tcgcggcggg
     2041 cttcttcaac attgacgtcg atacatcgac gttagtcgac gtgtccctcg ccacgctcga
     2101 cgagcagcaa cggctcaact atcaaacagc cgtcgacatc gcgcgctaca ttcgcgagat
     2161 cgaacccgac ggcgtgacga tttccgtggg aggcgagatc ggcgaagtgg gcgcgcacaa
     2221 cagcaccgtg ccggagctcc
//
LOCUS       DASUCJ010000907         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_223688, whole genome shotgun sequence.
ACCESSION   DASUCJ010000907 DASUCJ010000000
VERSION     DASUCJ010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223688"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFU90_10810"
     CDS             <1..745
                     /locus_tag="VFU90_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480316.1"
                     /translation="GVRQSGEPDPRYTRMTPVVEARGLRKVYVLGETQVVALDAVDLT
                     LDPGDFVAVMGPSGSGTSTFMQIVGLLDTPTAGTYRFEGGDVAHLSGDDRAGLRSRRL
                     GFIFQAYNLLPRTSALENVELPMVYAGVAEPRRRATALEKMELVGVAHLRDHNPNQMS
                     GGQQQRVAIARSLVNDPGLLLADEPTGALDTKSSADVMRLLKRLNDERGITIMLVTHE
                     PEIAAYARRIVTFRDGRIVADASNAQRAA"
     gene            742..1959
                     /locus_tag="VFU90_10815"
     CDS             742..1959
                     /locus_tag="VFU90_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480317.1"
                     /translation="MTLQATLRLALQALVRNKVRSLLTMLGIVIGVAAVIVTVAIGAG
                     ARISVQSSIDSLGSNLIVVQPGSVTESGARGGFGTASTLTPADGLAIATLPGVASVSP
                     VVTVRTQVVAGENNWQTTVGGVASTYTYIRSWPVSAGRFIGDADVASTAKVAVLGATV
                     VQQLFPDGRSPIGSTVIVKGAPFTVIGTLAPLGQSGLGQDQDDTVLIPYTSAMERLTG
                     LTTVNSLMVSAADSGDVDPVLQSLTLLLEQRHRIVPPQPDDFQVRNLQSIAQAASQTG
                     AVMEFLLAGVAAVSLLVGGIGIMNIMMVSVTERTREIGLRMAVGARAKTILQQFLTES
                     IVLATIGGLAGVLAGTAGTLLVASLAHWPTAIPPQWLAASVLFAAMVGIFFGYYPAQK
                     AARLHPIEALRFE"
     gene            complement(1962..>2685)
                     /locus_tag="VFU90_10820"
     CDS             complement(1962..>2685)
                     /locus_tag="VFU90_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5480318.1"
                     /translation="GVTTMRRVWMFGLLLVLAATAANAQAPAASVPPATQTATGIPLD
                     APWKVTIYQLARSKFVHPAWGWQHSERDYRVALSLATADRLTVDTDVLFAAAMLHDMA
                     AFVPCADAKMEHGDCAAQESEAVLRDAGFPMAKFAAVAAAERGHMYYRDPGTQPEAIV
                     LHDADSLDFLGDVGAARMIALTGAKSPSFAPAVKTLRAFVHDIPPHLITASGKRIGAR
                     RAAELTEFLDALDAETFGGKAM"
BASE COUNT          411 a          951 c          869 g          454 t
ORIGIN      
        1 cggcgttagg cagtccggtg agcccgatcc gcggtataca cggatgacgc cggtcgtcga
       61 agcgcgcggt ctgcgcaaag tctacgtatt gggcgaaacc caagtcgtcg cactcgacgc
      121 cgtggacttg acgctcgatc ccggcgactt cgtcgcggtg atggggccct cgggttccgg
      181 cacatcgacc ttcatgcaaa tcgtcggact gctcgacacg cccacggccg gaacgtatcg
      241 tttcgaaggc ggcgacgtcg ctcacctgag cggggacgac cgcgccggcc ttcgcagccg
      301 ccgcctcggt ttcatctttc aggcctacaa tctgctgccc cgcaccagcg cgctcgaaaa
      361 cgtcgagctg ccgatggtct acgccggcgt cgccgagcct cgccggcgcg cgaccgcgct
      421 ggaaaagatg gagctcgtcg gcgtcgcgca cttgcgcgat cacaacccca accagatgtc
      481 gggcgggcag cagcagcgcg tcgcgatcgc gcgatcgctg gtcaacgatc cggggctgct
      541 tctcgccgac gaaccgaccg gcgcgctcga taccaaatcg tcggccgacg tcatgcggct
      601 gctcaagcgg ctcaacgacg aacgcgggat tacgatcatg ctcgtgaccc acgagccgga
      661 gatcgccgcc tacgcccggc gcatcgttac cttccgcgac ggcaggatcg ttgccgacgc
      721 ctccaacgcg cagcgcgccg catgacgctc caagctacgc tgcggctcgc gctccaggcg
      781 ctggttcgca acaaggtgcg ctcgctgctc acgatgctcg ggatcgtaat cggcgtagcc
      841 gcggtgatcg tcacggtcgc catcggagcg ggcgcgcgca tctccgtgca aagctcgatc
      901 gacagcctcg gttcgaatct gatcgtcgtg cagccgggca gcgtcaccga gagcggcgcg
      961 cgcggcggtt tcggcacggc ctcgacgctc acgccggccg acgggcttgc gatcgcgacg
     1021 cttcccggcg tcgcgagcgt ttcgccggtc gtgacggtcc gcacgcaggt cgtcgccggc
     1081 gagaataact ggcaaaccac cgtcggcggc gtcgcctcga cctacaccta tatccgctcg
     1141 tggccggtct ccgccggccg gttcatcggc gacgccgacg tcgcctcgac cgcgaaggtc
     1201 gcggtgctcg gcgcgaccgt cgtccagcag ctcttcccgg acggccggtc tccgatcggt
     1261 tcgaccgtga tcgtgaaagg cgcgccgttt acggtgatcg gcacgctcgc accgctcggc
     1321 cagagcggcc tcggccaaga ccaagacgat accgtgctga ttccgtacac gtcggcgatg
     1381 gaacgcctga ccggcctcac caccgtcaac tcgctgatgg tttccgccgc cgattccggc
     1441 gacgtcgatc cggtcctgca atccctcacg ctgctcctcg agcagcgcca tcgcatcgtt
     1501 ccgccgcagc ccgacgattt ccaggtccgc aatttgcagt cgatcgcgca agccgcgtcg
     1561 caaaccggcg ccgtcatgga gtttctgctc gccggcgtcg cggccgtctc gctgctggtc
     1621 ggagggatcg gcatcatgaa catcatgatg gtctcggtga ccgagcgcac gcgcgagatc
     1681 ggcttgcgca tggccgtcgg cgcgcgcgcg aaaacgatcc tccagcagtt tctgaccgag
     1741 tcgatcgtgc tcgcaaccat cggcggactg gccggcgtgc tcgccggaac ggccggcacg
     1801 ctgctggtcg cttcgctggc ccactggccg accgcgattc cgccgcagtg gctggccgcg
     1861 tcggtgctgt ttgccgcgat ggtcggcata ttcttcggct actatccggc gcaaaaagcc
     1921 gcccggctgc atccgatcga agcgctgcgc ttcgagtagc gctacatcgc tttcccgccg
     1981 aacgtttcgg cgtcgagcgc gtcgagaaat tccgtcagct cggcggcgcg ccgcgcgccg
     2041 attcgttttc ccgaagcggt aatcaagtgc ggcggaatgt cgtgcacgaa cgcgcgcagc
     2101 gtcttcaccg ccggcgcgaa gctcggcgac ttcgcgccgg ttagtgcgat catccgcgcg
     2161 gcgccgacgt cgccgaggaa atccagcgag tcggcgtcgt gcaggacgat cgcttcgggt
     2221 tgcgtgcccg ggtcgcgata gtacatgtgg ccgcgctcgg cggccgccac cgccgcgaac
     2281 ttcgccatcg gaaaaccggc gtcgcgcagc accgcttcgc tctcttgggc ggcgcagtcg
     2341 ccgtgctcca tcttcgcatc ggcgcacggt acgaacgccg ccatgtcgtg cagcatcgcg
     2401 gccgcgaaca atacgtcggt gtcgacggtg aggcggtcgg cggtcgccag cgacagggcg
     2461 acccggtagt cgcgctcgct gtgctgccag ccccacgccg gatgaacgaa cttggagcgc
     2521 gccagctggt agatcgtcac cttccacggt gcgtcgagcg gaatccctgt cgccgtttgc
     2581 gtcgccggcg gcacgctcgc cgccggtgct tgcgcgttgg ctgcggttgc ggccagcacc
     2641 agcagcaacc cgaacatcca aacgcgccgc atcgtggtta ccccc
//
LOCUS       DASUCJ010000908         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_926776, whole genome shotgun sequence.
ACCESSION   DASUCJ010000908 DASUCJ010000000
VERSION     DASUCJ010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926776"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /gene="aceB"
                     /locus_tag="VFU90_10825"
     CDS             <1..1184
                     /gene="aceB"
                     /locus_tag="VFU90_10825"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU5480319.1"
                     /translation="RRIDYVSPEGKAYRLKEKVATLFVRPRGWHLPEKHVIVDGQPMS
                     GGIFDFALYFFHNAKELIARGSGPYFYLPKLESHLEARLWNDIFVMAQDDLGIPRGTI
                     KATVLIETIVAAFEMDEILHELRQHSAGLNAGRWDYIFSCIKKFRSNRDFCLADRARV
                     TMTTHFMKGYAELLIKTCHRRNAHAMGGMAAQIPVKNDEAANADAFAKVKADKEREAT
                     YGHDGTWVAHPALVIVAQQAFDRLMPAPNQIATRRRDDVEVTAADLVRFAPGQPITEQ
                     GLRLNISVAIQYVGAWLAGQGAVPIFNLMEDAATAEISRSQVWQWIRSPKGKLDDGRK
                     VTKELVAEFLPQEMAKIRELLGADYAAGRYEEAAKIFADLVYGDRFVEFLTLPAYDLI
                     D"
     gene            1355..1777
                     /locus_tag="VFU90_10830"
     CDS             1355..1777
                     /locus_tag="VFU90_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEU5480320.1"
                     /translation="MKPLKGTKTHDNLKFAFAGESQANRRYLYFANKADVEGQNDVSA
                     LFRSTAEGETGHAHGHLDYLAVVGDPVTDLPIGSSRDNLKSAVAGETHEYTDMYPGMA
                     KTARGEGFEEIADWFETLAKAERSHANRFQKALDGLSD"
     gene            1797..>2396
                     /locus_tag="VFU90_10835"
     CDS             1797..>2396
                     /locus_tag="VFU90_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480321.1"
                     /translation="MTIREGNLQAPTRHALDWRGAEFNDEAALLKEQERIFDICHGCR
                     RCVSLCSAFPTLFDLVDNSSTMEVDGVAKTDYPKVVEQCYLCDLCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAQKFRRGEVRLRDRMLTSTDAVGRLATIPVVVKAVNGGNRSSPMRK
                     LMERTLGVAADAWLPEYAPQPFLPHAAPSLKWPVRDGERT"
BASE COUNT          450 a          769 c          789 g          388 t
ORIGIN      
        1 ggcgccgcat cgactacgtg tccccggagg gcaaggctta tcggctgaag gagaaggtcg
       61 cgacgctctt cgtgcggccg cgcggctggc acctccccga gaagcacgtc atcgtcgacg
      121 gccagccgat gtcgggcggc atcttcgatt tcgcgctcta tttcttccac aacgcgaaag
      181 agctgatcgc gcgcggcagc gggccatact tctatctgcc gaagctcgaa agccatctcg
      241 aggcgcgtct gtggaacgac atattcgtca tggcgcagga cgatctcggc atcccccgcg
      301 ggaccatcaa ggcgaccgtg ctgatcgaga ccatcgtcgc ggcgttcgag atggacgaga
      361 tcctgcacga gctgcgccag cattcagccg ggctcaacgc cgggcgctgg gactacatct
      421 tcagttgcat caagaaattt cgcagcaacc gcgatttctg cctcgcggac cgcgcgcgcg
      481 tgacgatgac cacccacttc atgaagggct acgcggagct gctgatcaag acctgccacc
      541 gcaggaacgc ccatgcgatg ggcggcatgg ccgcgcagat tccggtgaag aacgacgagg
      601 cagcgaatgc cgacgcgttc gccaaggtga aagccgacaa ggagcgcgag gcgacctatg
      661 gccacgacgg cacctgggtc gcacacccgg cgctggtgat cgtcgcccag caggcgttcg
      721 atcggctgat gccggcaccg aaccagatcg cgacgaggcg ccgcgatgac gtagaggtga
      781 ctgcggccga cctcgtgcgc ttcgcgcccg ggcagccgat caccgagcag ggactgcggc
      841 tcaacatcag cgtggcgatt cagtacgttg gcgcgtggct cgcgggccag ggtgcggtgc
      901 caatcttcaa cctgatggag gatgcggcga cggccgagat ctcgcgctcg caggtctggc
      961 aatggatccg ctcgccgaag ggaaagctcg acgacggccg aaaggtgacg aaggagctgg
     1021 ttgccgagtt tcttccgcag gagatggcga agatccgcga gctcctcggc gccgactatg
     1081 cggcgggacg ctacgaagaa gcggcgaaga tcttcgccga cctcgtctac ggcgaccgct
     1141 tcgtggaatt cctcacgctg cctgcgtacg acctgatcga ctgataacga ggcgttatcg
     1201 tccgcattgc gacagtcaat tggacatatc gcggcggccg acggagaatc gcagcattgt
     1261 ccgcggctgg tggctgcttc gcgatcggcc gcggcattcg acccctcgcc cgcatcgggc
     1321 ggccgcaacg gtcacatcgt caaggagttc gcacatgaag ccactgaagg gtaccaagac
     1381 gcacgacaat ctaaagttcg cattcgccgg cgaatcgcag gcgaaccgtc gctacctcta
     1441 cttcgcgaac aaggccgacg tcgaggggca gaacgacgtt tcggcgctgt tccgctcgac
     1501 ggccgaaggt gagaccgggc acgcgcacgg gcatctcgac tacctcgccg tcgtcggcga
     1561 tccggtgacc gacctgccga tcgggtcgtc gcgcgacaat ctgaagtccg cagtggccgg
     1621 cgagacgcac gagtacaccg acatgtaccc gggcatggcc aagaccgcgc gcggcgaagg
     1681 cttcgaggag atcgccgatt ggttcgagac gctcgcgaag gccgagcgct cgcacgccaa
     1741 tcgcttccag aaggccctcg acggactcag cgactgatgc gtaccgtgcg tcggagatga
     1801 cgattcgcga gggcaatctg caggcgccga cgcggcatgc gctcgactgg cgcggcgccg
     1861 agttcaacga cgaggctgcg ctcctgaaag agcaggagcg catcttcgac atctgtcacg
     1921 gttgccggcg ctgcgtgtcg ctgtgcagcg cgttcccgac gctgttcgac ctcgtcgaca
     1981 acagcagcac gatggaggtc gatggggtcg cgaagaccga ctatccgaag gtcgtcgagc
     2041 agtgctatct ctgcgacctt tgctacatga cgaagtgtcc ctacgttcca ccgcatccgt
     2101 ggaacgtgga tttcccgcac ctgatgctgc gcgccaaggc gcagaagttc cgtcgcggcg
     2161 aggtgcggct ccgcgaccgc atgctgacgt cgaccgacgc ggtcggcagg ctcgcgacga
     2221 ttccggtcgt cgtgaaggcc gtcaacggcg gcaaccggtc ttctccgatg cgcaagctga
     2281 tggagcgcac gctgggcgtc gcggccgacg cctggcttcc cgagtacgcg ccacagccgt
     2341 tcctcccgca tgccgcgccg tcgctcaagt ggccggtgcg ggacggcgag cgcacg
//
LOCUS       DASUCJ010000909         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_406631, whole genome shotgun sequence.
ACCESSION   DASUCJ010000909 DASUCJ010000000
VERSION     DASUCJ010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406631"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFU90_10840"
     CDS             <1..1099
                     /locus_tag="VFU90_10840"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU5480322.1"
                     /translation="NALVERAIASADAMLPRAGRPPNVPAVCPPRERDGAFEFDNGSL
                     RARVTSGGEIVELSADGIPSAIERGNRLALYRDRPAKWEAWNVDADYVRFPEPLRMRT
                     PRIADGGLAIPFDAGTDSRGTLRLSIAEGEPFLRVDAAIDWRSRRRLLRVENDLRVDA
                     GEVTYGAPHGVVVRSAAADSPARRAQYEVPGQRFAAAWDRSTGNGVVSFALDTYGWNG
                     RAPEPGRLRLGHSLLRGTTWPDPHADAGEHQLAWAYAPVRAGGGIGSLERDWLRFACE
                     PSVRLFETGDDAVLIAACKPAEDGDGVVLRVRECDGAARRLRIRCGGRMREVRAVDAL
                     ERPLAGDAAIEAESIAASIGAFALRSFRVRF"
     gene            1132..1836
                     /locus_tag="VFU90_10845"
     CDS             1132..1836
                     /locus_tag="VFU90_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5480323.1"
                     /translation="MLFDLDDTLHDDTLAYQSAARDVAEEVAAAGGIDAAALKAAYVL
                     EAENFWKHLDADGLRVELAGVRRRLWQRALERVGITDPAIALRCARRYNDCRKRYFTP
                     FPGALELLQALRARGKRLGLVTNGFAETHREKIALLKLEELFEAVFIADEVGMLKPDP
                     QLFVHACERLGGEPKRAAMVGDRYDRDIRGAAEAGLFTVWFNVRGERLAAGSGPPPDA
                     VCTSLGEVGRVLDALG"
     gene            1906..>3503
                     /locus_tag="VFU90_10850"
     CDS             1906..>3503
                     /locus_tag="VFU90_10850"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480324.1"
                     /translation="MASGLDSIVIKGAREHNLKNVDLVLPRNRLIVVTGLSGSGKSSL
                     AFDTIYAEGQRRYVESLSSYARQFLGQMEKPDVDYIEGLSPAISIDQKSTSRNPRSTV
                     GTVTEIYDYLRLLYARVGTPHCYNCGREISTQSSQQIVDSIMELPEGTRFSLLAPLVR
                     GRKGEYSKLFEEAAKEGFARVRVDGQIKELREKIVLDKKRKHTIEVVVDRLVMKPEIR
                     QRLTDSVETTLRLSTGIVTVLTDDRELTFSEAYACVYCGLSFEELAPRLFSFNSPYGA
                     CPACSGLGEKIEIDPWKVIPDRSKPISDGAIVPWSRSLGTGKYPSMNPYYLQQLERVL
                     RRYRVKMTTPIDRMPEEVVDVILYGTEREQTFAYTSRAGKTWEYRASFEGVVNNLQRR
                     YAETSSDYVKEDIERYMSASTCNVCKGARLKPEALAVTLAGANIDALTRSTIEQLEGF
                     FRELHLTPRQEQIAHQIVKEIRARLGFLTNVGLTYLTLARSATTLSGGESQRIRLATQ
                     IGSALVGVLYILDEPSIGLHQRDNR"
BASE COUNT          584 a         1145 c         1216 g          558 t
ORIGIN      
        1 gaacgcgctc gtcgagcgcg cgatcgcttc cgccgacgcg atgctgccgc gcgccggccg
       61 gccgccgaac gtaccggccg tgtgcccgcc tcgcgagcgg gacggcgcgt tcgaattcga
      121 caacggatcg ctgcgcgcgc gcgtcacgtc cggcggcgag atcgtggaac tctcggcgga
      181 cggcattccg agcgcaatcg aacgcggcaa ccggctcgcg ctctatcgcg accgcccggc
      241 caaatgggaa gcctggaacg tggatgcgga ctacgtgcga tttccggagc cgctgcgcat
      301 gcgaacgccg cggattgccg acggcggact cgcgatcccg ttcgatgccg gaacggattc
      361 gcgcggcacg ctgcgtctct cgatcgccga aggcgaaccg ttcctccgcg tcgacgccgc
      421 gatcgactgg cgctcgcggc gccgccttct ccgcgtcgaa aacgatctgc gcgtcgacgc
      481 cggcgaggtg acgtacggcg caccccacgg cgtcgtcgtg cgcagcgcgg cggccgattc
      541 gccggcgcgg cgagcgcaat acgaggttcc cggacaacgc ttcgccgccg cgtgggatcg
      601 ctcgaccgga aacggcgtgg tgagttttgc gctcgacacg tacgggtgga acggccgggc
      661 gccggagccg ggcaggctgc gcttgggaca ctcgctgctg cgcgggacga cctggcccga
      721 tccgcacgcc gacgccggcg aacaccagct ggcgtgggcg tacgcacccg ttcgcgccgg
      781 cggcggaatc gggagcttgg aacgcgactg gctgcgcttc gcgtgcgagc cgtcggtccg
      841 gttgttcgag accggcgacg acgcagtgct gatcgcggcc tgcaaaccgg ccgaggacgg
      901 cgacggcgtg gtgctgcgcg tgcgcgaatg cgacggcgcc gcgcgccgct tgcggattcg
      961 ctgcggcggc cggatgcgcg aggtgcgtgc ggtagacgcg ctcgagcgtc cgctcgccgg
     1021 cgacgccgca atcgaagctg agtcgatcgc cgcatcgatc ggcgcgttcg cactgcgcag
     1081 cttccgggtg cgtttctgag cgcaccgggg ccggtccggc aggtcgacgt cgtgctgttc
     1141 gatttggacg atacgctgca cgacgacacg ctggcgtatc aaagcgccgc acgcgacgtc
     1201 gccgaagaag tggccgccgc cggcggtatc gatgcggccg cgctcaaagc ggcctacgtg
     1261 ctggaagcgg aaaacttctg gaagcacttg gacgccgacg gactgcgcgt cgagctggcg
     1321 ggcgtacgcc gccggctttg gcagcgagcc ctcgaacgcg ttgggattac cgacccggcg
     1381 atcgcgctgc gttgcgcgcg gcgctacaac gactgccgca agcgctactt cacaccattt
     1441 ccgggcgcgc tcgagctgct gcaggctttg cgcgctcgcg gcaagcggct gggtttggtg
     1501 accaacggct ttgcggagac ccatcgcgag aaaatcgcgc tgctgaaact cgaggaactg
     1561 ttcgaggcgg tcttcatcgc cgacgaggtc gggatgctca agccggatcc gcagctcttc
     1621 gtgcacgcct gcgagcggct cggcggggag ccgaagcgcg ccgcgatggt gggcgaccgg
     1681 tacgaccgcg acatccgcgg agccgccgaa gccgggcttt tcaccgtctg gttcaacgtg
     1741 cgcggcgagc gcctcgcggc cgggagcggg cccccgccgg atgccgtctg cacgagcctt
     1801 ggcgaggtcg gtcgcgtgct cgacgccctc ggatgagcgg ctcggcggga gcggctcgcg
     1861 gcgtttggaa ccttcggctc ctaggaaggg tctgttaagg ttcgtatggc ttcgggcctc
     1921 gattcgatcg ttatcaaagg cgcgcgcgag cacaacctca agaacgtcga tctcgtgctg
     1981 ccgcgcaacc ggctgatcgt cgtcaccggg ttgtcgggat cggggaagtc gtcgctggcg
     2041 ttcgatacga tttacgccga gggccagcgc cgttacgtgg agtcgctctc gtcgtacgcg
     2101 cggcagtttc tcggccagat ggaaaagccc gacgtcgact acatcgaggg cttgtcgccg
     2161 gccatctcga tcgatcagaa atccacctcg cgcaacccgc gctcgacggt cggaacggtt
     2221 accgagatct acgactacct gcgcctgctg tacgcgcgcg tcggaacgcc gcactgctac
     2281 aactgcgggc gcgagatcag cacccaatcg agccagcaga tcgtcgattc gatcatggag
     2341 ctgcccgagg gaacccgctt ttcgttgctg gctccgttgg tacgcggccg caaaggcgaa
     2401 tactcgaaac tgttcgagga ggccgccaag gagggcttcg cgcgcgtccg ggtcgacggg
     2461 cagatcaagg agctgcgcga gaagatcgta ctcgacaaga agcgcaagca cacgatcgag
     2521 gtcgtggtcg accggctcgt gatgaagccc gagatccggc aacggctcac cgactcggtc
     2581 gaaacgacgc tgcggctgtc gaccgggatc gtgaccgtgc tgaccgacga tcgcgagctg
     2641 accttcagcg aagcgtacgc ctgcgtgtac tgcggccttt cgttcgagga gctggcgccg
     2701 cggctgtttt cgtttaattc gccgtacggg gcgtgcccgg cgtgcagcgg cttgggcgaa
     2761 aagatcgaga tcgacccgtg gaaggtgatt cccgatcgca gcaagccgat atccgacggc
     2821 gcgatcgtgc cgtggagccg cagcctcggc accggcaagt atccgtcgat gaacccctac
     2881 tacctgcagc agctcgaacg cgtgctccgg cgctaccgcg tgaagatgac gacgccgatc
     2941 gaccggatgc cggaagaggt cgtcgacgtc atcctgtacg gcaccgagcg cgagcaaacg
     3001 tttgcgtaca cgtcgcgcgc ggggaagacc tgggagtatc gcgcctcgtt cgaaggggtc
     3061 gtcaacaacc tgcagcggcg ttacgccgag acgtccagcg actacgtgaa ggaagacatc
     3121 gaacgctaca tgtcggcctc cacctgcaac gtctgcaaag gggcgcgact gaagcccgaa
     3181 gcgttggcgg tgacgcttgc cggcgcgaac atcgatgcgt tgacgcgctc gacgatcgaa
     3241 caactggagg gtttcttccg ggagcttcac ctcacgccgc gccaggagca gatcgcgcac
     3301 caaatcgtca aggaaatccg cgcgcggctc ggctttctca ccaacgtcgg gctgacgtac
     3361 ctcacgctgg cgcgctcggc cacgacgctc tcgggcggcg aatcgcagcg catccggctc
     3421 gcgacccaga tcggcagcgc gctggtggga gtgctctaca ttctcgacga gccgtcgatc
     3481 gggctgcatc agcgcgacaa ccg
//
LOCUS       DASUCJ010000910         1386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_498363, whole genome shotgun sequence.
ACCESSION   DASUCJ010000910 DASUCJ010000000
VERSION     DASUCJ010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498363"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFU90_10855"
     CDS             <1..775
                     /locus_tag="VFU90_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480325.1"
                     /translation="FPGVFPCFDDPTIGDLLDRARVSWKYYTGSQDAVADAWVNVYDA
                     IRHVRYGPDWQTNVVTPMSNVLADIQNCNLPQVAYVTPTWMASDHAGSMSNAGPGWVG
                     SIYMALEQTARTASAGCRYAGNTAMIVTWDDTGGWYDHVKPPSGPDGTTLGFRVPIVV
                     ISPWARSNYVPGDSHFMPFVSHTVRSSTAIVRFIEKNWALGTLGQRDAAGDDLSDMFD
                     YARAAPVPPIIELAVRNLVHKTQWNLARSLHDDHVVDDQ"
     gene            777..>1386
                     /locus_tag="VFU90_10860"
     CDS             777..>1386
                     /locus_tag="VFU90_10860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5480326.1"
                     /translation="MSAQAARTDLRGVRLAVLASIAWPAPPAGYGPWEQIAYDIATGM
                     LRRGLDVTLFATGNTRFDGKLVSVVPVGLNEDPALSGDVFGALHIGELFRRAADFDLI
                     HNNFDWKPLTYALATEAPPLLTTVHGFSSPQILAAYYACAWRSFFCSISNADRDPGLD
                     YLATTYNGVDPAAFPFSDEPGDYLVLLGRFHPEKGTHLAIDVA"
BASE COUNT          231 a          475 c          427 g          253 t
ORIGIN      
        1 gttcccgggg gtctttccgt gcttcgacga tccgacgatc ggcgatctgc tcgatcgcgc
       61 acgcgtgtcc tggaaatact acaccggatc gcaggacgcg gtggccgacg cgtgggtgaa
      121 cgtttacgac gcgatccggc acgtccgtta cgggcccgat tggcaaacca acgtcgtcac
      181 gccgatgtcg aacgtgctcg ccgatatcca aaactgcaat ttgccgcaag tggcctacgt
      241 aacgccgacc tggatggcgt cggatcacgc cggctcgatg agcaacgccg ggcccggctg
      301 ggtcggctcg atttatatgg cgctcgaaca aaccgcccgc accgcctcgg cgggctgccg
      361 ctacgccggc aataccgcca tgatcgtcac ctgggacgac accggcggat ggtacgatca
      421 cgtcaagccg ccgagcggcc ccgacggcac gaccttgggc ttccgcgtcc cgatcgtcgt
      481 aatttcgccg tgggcgcgct ccaactacgt gcccggggat tcgcacttca tgccgttcgt
      541 ctcgcatacc gtgcggtcgt cgacggcaat cgtgcgcttc atcgaaaaaa attgggcgct
      601 cggaacgctc gggcagcgcg acgccgccgg cgacgatttg agcgacatgt tcgattacgc
      661 gcgcgccgcg ccggtaccgc cgattatcga gctcgcggtg cgaaatctcg tccacaagac
      721 ccagtggaat ctcgcccgca gcctccacga cgatcacgtc gtcgacgacc agtagggtga
      781 gcgcgcaggc cgcacgcacc gatttgcggg gagtccggct tgcggtgctc gcctcgatcg
      841 cgtggcccgc gccgcccgcc ggctacggcc cttgggaaca gatcgcctac gacatcgcga
      901 ccgggatgct gcggcgcggt ctcgacgtca cgctgttcgc taccggaaat acccgtttcg
      961 acggcaagct cgtttcggtg gttccggtgg gcctgaacga ggatccggcg ctatcgggcg
     1021 acgtgttcgg cgcgctgcac atcggcgagc tgttccgccg ggcggccgac ttcgatctga
     1081 tccacaacaa cttcgactgg aagccgctca cctacgcgct cgcaaccgaa gctccgccgc
     1141 tgctgacgac cgtgcacggc ttctcgtcgc cgcaaattct tgcggcttac tatgcctgcg
     1201 cgtggcgcag cttcttctgt tcgatctcca acgccgaccg cgatcccggg ctcgactact
     1261 tggcgacgac ctacaacggc gtcgacccgg cggcgtttcc cttcagcgat gagccgggcg
     1321 actatctggt gttgctcggg cgcttccatc ccgaaaaagg gacgcatctc gcgatcgacg
     1381 tcgcca
//
LOCUS       DASUCJ010000911         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_820011, whole genome shotgun sequence.
ACCESSION   DASUCJ010000911 DASUCJ010000000
VERSION     DASUCJ010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820011"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFU90_10865"
     CDS             complement(<1..426)
                     /locus_tag="VFU90_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEU5480327.1"
                     /translation="MDKVVSSCAEAVKDIPSGASIAVGGFGLNGIPHNLIQALLEQGA
                     SGLVTVSNNCGVDGWGLGVLLDARRIRRTTGSYVGENKEFERQYLQGELEVELVPQGT
                     LAERLRAGGCGIPAFYTLAGVGTQVADGGLPWKYASDGSI"
     gene            559..>1214
                     /locus_tag="VFU90_10870"
     CDS             559..>1214
                     /locus_tag="VFU90_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5480328.1"
                     /translation="MILDAVRELVAEKVAPRAAEIDERGEYPQDIRKLFAENDLLGIP
                     IPAEYGGLGGTFLTYVKVVEEIAKACASSSLIVAVQELGMLPILIGGSEDQKHKYLPK
                     LASGEWIAAYALTEAGSGSDAAGSMRTRAEKRGDKYVLNGQKIWITNGSVADVVCVFA
                     VTDPGKGASGISAFIVEKDFPGFNVGKLEKKMGIRGSPTVELSFADCVVPAANRLGDE
                     G"
BASE COUNT          234 a          400 c          383 g          197 t
ORIGIN      
        1 gatgctgccg tcgctcgcgt acttccacgg aagtccgccg tcggcgactt gcgttccgac
       61 gccggcgagc gtatagaatg cgggaatgcc gcacccgccc gcgcgcaggc gctcggcgag
      121 cgtgccttgc ggcacgagct cgacttccag ctcgccttgc agatactggc gctcgaattc
      181 tttattctcg ccgacgtacg agccggtcgt gcgcctgatg cgcctcgcat cgagcagcac
      241 gccgaggccc cagccgtcga cgccgcaatt gttggacacc gtgaccaggc cgctcgcgcc
      301 ttgctcgagg agcgcttgga tcaaattgtg cggaatgccg ttgaggccga acccgcccac
      361 ggcgatggat gcgccgctgg ggatgtcttt gaccgcctcg gcacacgaag agacgacttt
      421 gtccatattt ggcggtgata tgccagcggg gacggcgggt cccgccccgc gaagttccgc
      481 ctacccaatt accactagga gatccgaacg ccaatggcag tcgccacccc gcatcactcc
      541 tcgtcagacg aagaggcgat gatcctcgac gccgtgcgcg agctcgttgc ggaaaaagtc
      601 gcacctcgcg ccgctgaaat cgacgagcgc ggcgaatacc cccaagacat tcgcaaactc
      661 tttgccgaga acgacctgct cgggattccg atccccgccg aatacggcgg cctgggcgga
      721 accttcctga cctacgtcaa ggtcgtcgag gagatcgcca aagcgtgcgc gtccagctcg
      781 ctcatcgtcg cagtccaaga gctgggcatg ctgccgattc tgatcggcgg cagcgaggat
      841 cagaagcaca agtatctgcc gaagctcgcg agcggcgaat ggattgccgc atacgcgctg
      901 acggaagcgg gcagcgggtc ggacgccgcc ggctcgatgc gcacgcgcgc cgagaaacgc
      961 ggcgacaagt acgtcctgaa cggccaaaag atctggatca ccaacggcag cgtcgccgac
     1021 gtcgtgtgcg tcttcgccgt aacggatccc ggaaagggcg caagcggcat cagcgcgttc
     1081 atcgtcgaga aggacttccc gggattcaac gtcggcaagc tcgagaaaaa gatgggcatc
     1141 cgcggatcgc cgacggtcga gctgtcgttc gccgactgcg tcgtgcccgc cgccaatcgc
     1201 ctcggcgacg aagg
//
LOCUS       DASUCJ010000912         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_315180, whole genome shotgun sequence.
ACCESSION   DASUCJ010000912 DASUCJ010000000
VERSION     DASUCJ010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315180"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFU90_10875"
     CDS             complement(<1..233)
                     /locus_tag="VFU90_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480329.1"
                     /translation="MPSYVRAGSLPHKRHIQFRRPDGGLYAEELFSMKGFESAYSLLY
                     HERPPTATLEIRPWERPAVHFVENDPLRNRHFKT"
     gene            complement(233..466)
                     /locus_tag="VFU90_10880"
     CDS             complement(233..466)
                     /locus_tag="VFU90_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU5480330.1"
                     /translation="MQEVVIKVRESGPYIVRGPITLTDADGNPYTTGEGNVALCRCGG
                     SQTKPFCDGSHRTNGFSATHRAFSPLVYDAGER"
     gene            511..1509
                     /locus_tag="VFU90_10885"
     CDS             511..1509
                     /locus_tag="VFU90_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480331.1"
                     /translation="MKWTLAGLAALAVAAFLPPAFNTGATVAAAATPTPAALQYDELT
                     RMVIPPATPPAPGTFAADYQVAASATPIPKLPSLNFGMMINPAAAKKYADQMQAMAAQ
                     VRVTRYTFYRGWIRREDPVARTAEIEKCDRHQYIKLDLANKTYTSISAEAACATPQAA
                     MPGPPQASGEAGGTVDLVTTVTNTDLGPMTLDGIPTTGFDRSVELKLTNATGSCRDGD
                     LKMTVRQYVSNVKVPHRFCPLPKGMNPLSAAGSSPGCKPKIAGLENGNFSIDTMGVDV
                     GSLDNLVMFSRLSADVGGRGAGFGMFVERGNVRWFGGQDAEALFVIPPGFTQTSGS"
BASE COUNT          276 a          511 c          528 g          279 t
ORIGIN      
        1 gttttgaaat ggcggttgcg cagcgggtcg ttttcgacga agtggacggc cgggcgttcc
       61 cacggacgaa tctcgagcgt ggccgtcggc ggccgctcgt gatacagcag cgagtacgcg
      121 ctctcgaatc ccttcatgga aaacagctct tcggcgtaga gtccgccgtc gggccgccga
      181 aactgaatgt gccgcttatg cggaagcgaa ccggcgcgta cgtacgacgg catcagcgct
      241 cccccgcgtc gtagaccagc ggcgagaacg cccggtgggt tgccgaaaag ccgttggtgc
      301 gatgcgatcc gtcgcagaac ggcttggtct gcgagccgcc gcagcggcac agcgcgacgt
      361 taccctcgcc ggtcgtgtac gggtttccgt cggcgtcggt gagggtaatc gggcctcgca
      421 cgatgtacgg cccgctctcg cggactttta tgacgacttc ctgcatgacg gaccaggcgt
      481 tcgacgcgtt cgcggcgcaa tccggtagtc atgaagtgga cgctagcagg tcttgcggcg
      541 cttgcagtcg ccgctttcct tccgccggct tttaacacgg gtgcgacggt cgcggcggcc
      601 gcgacaccga cgccggcggc actgcaatac gacgagctca cgcggatggt catcccaccg
      661 gcgacgcccc cggcgcccgg gacgtttgcc gccgattatc aagtggccgc ttcggccacg
      721 ccgataccga agcttccgag cctcaatttc gggatgatga tcaatcccgc cgcggcaaag
      781 aagtacgcgg accagatgca ggcgatggcg gcgcaggtac gcgtgacgcg gtatacgttc
      841 taccgcggct ggatacgtcg cgaggatccg gtggcccgca ccgccgagat cgaaaaatgc
      901 gaccgccacc agtacatcaa gctcgatctt gccaacaaaa cctacacgtc gatcagcgcg
      961 gaagccgcct gcgcgacgcc ccaggcggcg atgcccggtc cgccgcaagc ctcgggcgag
     1021 gccggcggta cggtcgatct cgtcacgacc gtcaccaaca ccgatttggg tccgatgacg
     1081 ctcgacggga ttccgaccac cggcttcgac cgcagcgtcg agctcaagct gaccaacgcc
     1141 accggttcat gccgggacgg cgatttgaag atgacggtcc ggcaatacgt ctcgaacgta
     1201 aaggttccgc atcgtttttg cccgctgccc aaaggcatga acccgctgtc ggcagcgggg
     1261 tcgtcaccgg gctgcaagcc gaaaatcgcc gggctcgaaa acggcaactt cagcatcgac
     1321 acgatgggcg tcgacgtcgg ctcgctggat aatctcgtga tgttctcgcg cctgtcggcc
     1381 gatgtgggcg gtcgcggtgc gggattcgga atgttcgtgg agcgcggaaa cgtccgttgg
     1441 ttcggcggcc aggacgccga ggcgctgttc gtcatccctc ccgggtttac gcagacctcc
     1501 gggtcgtagc gtttagggtc gcgcggcggt cgcctccagc accgcgcgcg ccaggacgtg
     1561 ggagcggacc ttggcttcga gttgcggtcc cgtg
//
LOCUS       DASUCJ010000913         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1018458, whole genome shotgun sequence.
ACCESSION   DASUCJ010000913 DASUCJ010000000
VERSION     DASUCJ010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018458"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..>1118)
                     /locus_tag="VFU90_10890"
     CDS             complement(34..>1118)
                     /locus_tag="VFU90_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5480332.1"
                     /translation="IDTACSSEQGILGLAFDPDYATNGTFYVTYTAPNSDPELGAAND
                     QLLARFTVSSDPDVANPDPEVLLRVPDIADNHNGGDLAFDSDGYLDWSVGDGGVQGDP
                     NGFAQCTGRKKADNDPSSCYDTTGSDPNYYLLGKIIRLDVHGTIASAASNICGVAAGQ
                     PVPYAIPPGNPFADAGAHPNDCAEIFNWGFRNPFRFSFDRQTGDMLIGDVGQNTYEEI
                     DFQPAGSAGQNFQWNECEGFHTYPGGTAPCAGPPGSVPPKLEYSHSFGCAVVGGFRYR
                     GPIGPFRGQYVFSDNCSGGIYIVADADGSVPVWTSEQLGGTPGMSPYSFGEDEAGNVY
                     VADGNGPVYIFHSDSGSDVIFQNGFD"
BASE COUNT          176 a          356 c          381 g          205 t
ORIGIN      
        1 gttgcgaaag cgccacccgt ccgcggatcg atttcagtcg aatccgttct ggaagatgac
       61 gtcgctgccg ctgtcggaat ggaagatgta gacgggcccg ttgccgtcgg cgacatagac
      121 attgccggct tcgtcctcgc cgaagctata tgggctcatg ccgggcgtgc cgcccagctg
      181 ctcggacgtc cagaccggta cgctgccgtc cgcgtccgcg acgatgtaga tgccgccgct
      241 gcagttgtcg ctgaacacgt actggccgcg gaacggcccg atcgggccgc gatagcggaa
      301 gccgccgacg accgcgcaac cgaacgaatg ggaatactcg agcttcggcg gcacgctgcc
      361 cggcggtccc gcgcagggcg ccgtgccgcc gggatacgtg tggaaacctt cgcattcgtt
      421 ccactggaag ttctggcccg cgctgccggc cggctggaag tcgatttcct cgtaggtgtt
      481 ctggccgacg tcgccgatca gcatgtcgcc ggtctgccgg tcgaagctga agcggaacgg
      541 attgcggaag ccccagttga agatctccgc gcagtcgttc ggatgcgcgc ctgcgtccgc
      601 gaacggattg ccgggcggga tcgcgtacgg caccggctgc cccgccgcga cgccgcagat
      661 gttcgatgcc gccgacgcga tcgtgccatg cacgtcgagc cggatgatct tgccgagcag
      721 gtaatagttc ggatcgctgc cggtcgtgtc gtagcagctc gatggatcgt tgtcggcttt
      781 cttgcgcccc gtgcattgcg cgaaaccgtt cggatcgccc tgcacgccgc cgtcgccgac
      841 cgaccagtcg agatagccgt ccgaatcgaa cgcgagatcg ccgccgttgt gattgtcggc
      901 gatatcgggg acgcgcagca gcacctcggg atcgggattg gcgacgtcgg gatcgctcga
      961 caccgtgaag cgcgcgagga gctggtcgtt tgccgcgccg agctctggat cgctgttcgg
     1021 cgccgtgtat gtcacgtaga acgtgccgtt cgtcgcgtag tcggggtcga acgcaaggcc
     1081 gaggatgccc tgctcgctcg agcacgccgt gtcgatcg
//
LOCUS       DASUCJ010000914         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_926854, whole genome shotgun sequence.
ACCESSION   DASUCJ010000914 DASUCJ010000000
VERSION     DASUCJ010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926854"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFU90_10895"
     CDS             <1..412
                     /locus_tag="VFU90_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5480333.1"
                     /translation="AGQSLNIISMIGIVMLFGLVSKNGILLVDYSNTLCRRGMRVRDA
                     VLQAASTRFRPILMTTCAMIFGMLPLSLGFAEGGEWRQAMGTVIIGGLASSLILTLFL
                     VPMIYNTWIGWAERRADHRAIEAEMSQPAAPARA"
     gene            488..1240
                     /locus_tag="VFU90_10900"
     CDS             488..1240
                     /locus_tag="VFU90_10900"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5480334.1"
                     /translation="MNAIRHISLAARTAAVVAAAALAVVSSFAPQAASAQGVTVIVNG
                     QTMSFDQPPIVQAGRVFVPLRGVFENLGASVVYANGQINATGRGRTVSLTIGSTQATV
                     DGQPVTIDVAPFIVGARTMVPLRFIAQSLGASVDWNNNTSTVTINAGRGERVYPGQNP
                     VTFGYQWPTGTVYSHYPQIRFQVNRQVDIGGFQVLVDGQDITSGVQSNGRYYYVATPF
                     SLPVGNHRVRVRGRTAGGVPFDLSWTFYQASY"
BASE COUNT          230 a          430 c          426 g          238 t
ORIGIN      
        1 ggccggccaa tcgttgaaca tcatctcgat gatcggcatc gtcatgctct tcggcttggt
       61 atccaagaac ggaatattgc tggtcgacta ctcgaacacg ctgtgccggc gcggcatgcg
      121 cgttcgcgac gcggtgctgc aagccgcctc gacgcgcttc cggccgattc tcatgacgac
      181 gtgcgcgatg atcttcggca tgctgccgct gtcgctgggc ttcgccgaag gcggcgagtg
      241 gcggcaagcg atgggtaccg tcatcatcgg cggtctcgcc agctcgctga ttcttaccct
      301 cttcctcgtg ccgatgatct acaacacgtg gatcggctgg gccgagcggc gtgcggatca
      361 ccgtgcgatc gaagccgaga tgtcccagcc ggccgcgccg gcgcgcgcct aaaaggtccg
      421 ttagaagcgg ctctccagta agcgcttagt cgattggcgg cgagcaagcg cgggtagaaa
      481 gccgtgcatg aacgcaatac ggcatatctc tttggcggcg cgcacggcgg ccgtcgtcgc
      541 cgcggccgcg ctcgcggtcg tctcgagctt cgctccacaa gccgcctcgg cccaaggcgt
      601 aacggtaatc gtcaacgggc aaacgatgag cttcgaccaa cccccgatcg tgcaggccgg
      661 ccgggttttc gtgccgctgc gcggcgtttt cgaaaacctc ggcgcgagcg tggtctacgc
      721 taacgggcag atcaacgcga ccggccgcgg gcgtacggtc tcgctgacta tcggctccac
      781 gcaagctacc gtcgacggtc aacccgtcac gatcgacgtg gcgccgttca tcgtcggcgc
      841 gcgaacgatg gttccgctgc gcttcatcgc gcagtcgttg ggtgcgagcg tggactggaa
      901 taacaacacc agcacggtga cgatcaacgc aggccgcggg gaacgcgtct atccggggca
      961 aaatccggtg acgttcggct accagtggcc gacgggaacg gtctacagcc actatccgca
     1021 gattcgcttc caggtgaatc gtcaggtcga catcggcggg ttccaagtgc tggtcgacgg
     1081 tcaagacata accagcgggg tgcagagcaa cggccgctac tattacgtcg caacgccgtt
     1141 ctcgctgccg gtaggcaatc accgagtgcg cgtacgcggc cgcacggccg gcggcgtgcc
     1201 gttcgacctg agctggacct tctaccaggc ctcgtactaa tcggcgaatc cgcgcttcgc
     1261 gcatccccac cgctccgacg caaccgcgcg acggtggggg tgccgtcgct cggacgttcg
     1321 gagc
//
LOCUS       DASUCJ010000915         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1079673, whole genome shotgun sequence.
ACCESSION   DASUCJ010000915 DASUCJ010000000
VERSION     DASUCJ010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079673"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..911
                     /locus_tag="VFU90_10905"
     CDS             117..911
                     /locus_tag="VFU90_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5480335.1"
                     /translation="MTQGPRRLALDDGAITTLECWGERGPLVLAVHGMTSSRKSWQRL
                     ARHLDGRYRVVAYDQRGHGDSAGVEGPMSLERGVRDLENVVAALGEPVDTLVGHSWGG
                     AVAILAAPHLPVRRVVAIDPMIHQADAAWYEEYLEELREQFAFTGSARDARTREAYAA
                     WDPVDVEAKVHAVHVMTTVPIERLMRENPPATWDLRPAIAAFPKPLLLAMADAGEGIN
                     DAGPLEAVANERSKSVDLRSFPGAGHNLHRTAFDAFASAVDEFLER"
     gene            complement(908..1417)
                     /locus_tag="VFU90_10910"
     CDS             complement(908..1417)
                     /locus_tag="VFU90_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5480336.1"
                     /translation="MKIGIIGSDPRAIAIGRLLASGGHELTVGDVRADAAGRAAAHIG
                     APVETPYQQAMTRDVLVMAFPRSEADRVLAAMGASPHGVIVDAREGGPARPHHGAELL
                     AHKLDSHEVVRALVVLPQQGANIPICGDDPEAKAVVNEVFTACGCLTADRGPLSNAAE
                     LEPAGTDAA"
     gene            1651..>2001
                     /locus_tag="VFU90_10915"
     CDS             1651..>2001
                     /locus_tag="VFU90_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480337.1"
                     /translation="MIDVNNFDAMRIGLASPEQIRAWSFGEVKKPETINYRTLKPERD
                     GLFCEKIFGPTKDWECHCGKYKRIRFKGMICDRCGVEITRAKVRRERMGHIELATPVS
                     HIWYLKGVPSRIGIL"
BASE COUNT          345 a          641 c          682 g          333 t
ORIGIN      
        1 gagctggcca agagcatgcg cgaatcgctg ggcttgaaaa acgagggcgt caacgacgcg
       61 gtgacgttga actcgctcga cgacctgcac gaactgcaca aacaagtcta cagcgggtga
      121 cccaggggcc gcggcggctg gccctcgacg acggcgcaat tacgacgctc gaatgctggg
      181 gcgagcgggg gccgctggtg ctcgccgtcc acggtatgac cagctcgcgg aaatcttggc
      241 agaggctggc acgccacttg gatgggcgat accgcgtggt cgcttacgac cagcgcggtc
      301 acggggatag cgccggcgtc gaagggccga tgtcgttgga gcgcggcgtt cgcgatttgg
      361 aaaacgtcgt cgccgcactc ggcgaaccgg tcgatacgct cgtcggacac tcgtggggag
      421 gcgcggtcgc gattcttgcg gcgccgcatc tgccggttcg ccgggtcgtc gcgatcgatc
      481 cgatgattca tcaggcggac gcggcatggt acgaagagta cctggaggag ctgcgcgagc
      541 agtttgcctt taccggttcg gcgcgcgacg ctcgcacgcg ggaagcctat gcggcgtggg
      601 acccggtcga cgtcgaggcc aaggttcacg ccgtgcacgt catgacgacc gtgccgatcg
      661 aacggctcat gcgcgaaaat ccgccggcaa cctgggatct gcgtccggcg atcgccgctt
      721 tccccaaacc gctgctgctc gcgatggccg acgccggcga ggggatcaac gacgccggcc
      781 cgcttgaggc cgtggcgaac gaacgatcga agtccgtcga tctgcgcagc ttcccagggg
      841 cgggccataa tctgcatcgg acggcgttcg acgcgttcgc atcggcggtg gacgagtttc
      901 tggaacgcta ggccgcgtcc gtcccggcgg gctcgagctc ggccgcgttc gacagcggcc
      961 cgcggtcggc ggtcaggcag ccgcacgccg tgaacacctc gttgacgacg gccttcgcct
     1021 ccggatcgtc gccgcagatc gggatgttgg cgccctgttg cggcagcacg acgagcgcgc
     1081 gcacgacctc gtgcgaatcg agcttgtgag ccaatagctc ggcgccgtga tgcggcctcg
     1141 ccgggcctcc ttcgcgggcg tcgacgatga cgccgtgcgg gctggcgccc atcgctgcga
     1201 gcacccggtc cgcctcagac cgagggaagg ccattaccag gacgtcgcgg gtcatggctt
     1261 gctggtacgg cgtctccacg ggagccccga tgtgcgccgc ggcgcggccg gcggcgtcgg
     1321 cgcgcacgtc cccgaccgtg agctcgtgac ctccggaagc aagtaaccgg ccgattgcaa
     1381 tggcccgtgg gtccgatccg atgatgccaa tcttcatgcc taggtcgtac ccgcaaaacc
     1441 taggcgctca tgcgaagtga ccgccggctt ggccgcggca ctctggaagc gcttccgggc
     1501 gctcttgctg gtgaccaccc gccgtggtaa actacctttc tgtgccccgt gccagcagcg
     1561 acggtgggag cccttacgtg acgacgtgag cagcgatctc cctgcgtaag cagcggcagc
     1621 ggcgggccgg cggagtcctt ttccttgaat ctgatcgacg tcaacaactt cgacgcgatg
     1681 cgtatcggcc tcgcgtcccc ggagcaaatt cgcgcgtggt ccttcggcga agtcaagaag
     1741 cccgaaacga tcaattaccg gacgctcaaa ccggagcgcg acggcttatt ctgcgaaaag
     1801 atctttgggc caaccaaaga ctgggagtgc cactgcggca agtataagcg gatccgtttc
     1861 aagggaatga tttgcgaccg ctgcggcgtc gagatcaccc gcgccaaggt tcgccgcgag
     1921 cgcatgggcc acatcgagct ggcaacgccg gtgagccaca tttggtatct gaagggcgtt
     1981 ccaagccgca tcggcatcct g
//
LOCUS       DASUCJ010000916         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_514224, whole genome shotgun sequence.
ACCESSION   DASUCJ010000916 DASUCJ010000000
VERSION     DASUCJ010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514224"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFU90_10920"
     CDS             complement(<1..656)
                     /locus_tag="VFU90_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480338.1"
                     /translation="MTRTWRCALASAALAALAAGTGAARADAPQVAGSPIAHVIVVIQ
                     ENRTTDNLFASSVLAGGGPYPGADVTQTAVVDGKRVALKAVPFEYPADPRHTHPALLA
                     EWNNGKMDGFAKDPVYPDPGFPPGPPNFPLAYVPASETTMYHLLADRYALADENFAPR
                     LVPTFPGHLFLVTAQSQAADDPNDPITWGCDSKPGTTVPVFGAGETETFPGVFPCFDD
                     P"
     gene            complement(653..1801)
                     /locus_tag="VFU90_10925"
     CDS             complement(653..1801)
                     /locus_tag="VFU90_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480339.1"
                     /translation="MIVRAGILYDGTLEPPKRNVDLAIAGGRIAELRAAGGACDFEAA
                     CVTPGLINAHAHVEMSGEPDTMAAIAARRPNERLIAAVENARKSVAAGVTTIRDVGSS
                     HRIAADLRDAIDAGRIPGPRMRAAGEVLCMTGGHGWPVGRAVDSPWDARKAVREQLWA
                     GADCIKLIATGGVLTKGAVPGNAQLTPDELAAAVDEAHRHGMRVAAHAIGTQGIKNAL
                     RAGVDSIEHGHMLDDEAIELFKSRHAFLVPTLTAPTCILAHLEDGRQPQHVLDKARTV
                     NEAMLANVRRAYENGVRIAGGSDAGTPYNYHENYAYEVELMWSLLGMTPQQALHAATA
                     VAAELIGLHGGTLAVGEPADLLLLARDAGEDVRALRDVQTVIKGGEVV"
     gene            1892..2431
                     /locus_tag="VFU90_10930"
     CDS             1892..2431
                     /locus_tag="VFU90_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480340.1"
                     /translation="MKRPLPLWLAAVLPATIAAHALGYAVTGQSMADGRHLWLVPAFE
                     SSLAVLLLLATALIGGALVRAGVFARPSVEAAPLDLWLRLSAAQTALFVAIERAEGTP
                     VGLIGCAIQIAVALGAALLLAAFARALAGCVRTGDVAARFLTRAVAAPVYLGRPVSRA
                     DVLAAVAGSRRFQRPPPPR"
     gene            2472..4253
                     /locus_tag="VFU90_10935"
     CDS             2472..4253
                     /locus_tag="VFU90_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5480341.1"
                     /translation="MLRLRAVTFPGILFVAILAAGWWPARALAVPVFANGQGVSCETC
                     HTTFPGMTRYGMMVMMTNFQILNRHLQDQALPLAARLYITSYLANHSQPGSIAVSDLS
                     LLAAGFMGRNFTYYAEQHVIDDAQIGDTEQLWLSWNGLLHGTNSIQAGKFHTPFPFMP
                     AHAWTLADYLLATQTTGQNDWNPNDARWGFAFNGMSNEFMYNAAYLTGDGPTSEAFDY
                     NSQTQPRTVDLNVSYGGMSEPWSVGLVGIWGQAPLHDAATNLFDGQDPFSREGLYFGY
                     QTDAWHLQTMYYHGFDARPDTGAFDIPLNGFFLEIERDFGWRNHVVVRYDVASSDTLD
                     QQYLIDVSHNVQPNLALIGELLVGPQHVPQIGFQIAYAGPYEMGKRFVWQPSGGSDSI
                     AKGIAVVPAGANAAPSSLPAPAGSGAQSTGADANDGARLVQANGCTGCHGAQWQGGVG
                     PALFGIEHRMSPLEIALHIKSPAAPMPNFGFTDAQVADIVAYLSSLDGGGAQAGPVVT
                     FDPSTPVKNATIRVVFAGTPPHRVTALPTMQMGAGKMQTQAVTLQPSPSDPHTFTGTI
                     DFSMGGPWIVIIEYDGTTMSVPLNVGM"
BASE COUNT          659 a         1498 c         1455 g          776 t
ORIGIN      
        1 ggatcgtcga agcacggaaa gacccccggg aacgtttcgg tttcgccggc gccgaaaacc
       61 ggtaccgtcg tgcccggttt cgaatcgcag ccccaggtga tcggatcgtt cggatcgtcg
      121 gccgcttggc tttgcgccgt caccaggaac agatggcccg gaaacgtcgg caccagccgc
      181 ggcgcgaagt tttcgtcggc cagcgcgtag cggtcggcca gcaagtggta catcgtggtt
      241 tcgcttgccg gcacgtacgc caatgggaaa ttcggcggcc ccggcgggaa gccgggatcc
      301 ggatacaccg gatccttggc aaacccgtcc atcttcccgt tattccactc cgcgagcagc
      361 gccgggtggg tgtggcgcgg atccgccgga tactcgaagg gcaccgcttt gagtgcgacg
      421 cgtttaccgt cgacgacggc cgtctgcgtg acgtctgcgc cgggatacgg cccgccgccg
      481 gcgagcaccg acgacgcgaa cagattgtcg gtcgtccggt tttcttggat aaccacgatc
      541 acgtgcgcga tcgggctccc cgcaacttgc ggtgcgtcgg ctctggcagc gccggttccg
      601 gcggccaatg ccgccagcgc cgcgctcgca agcgcgcatc gccacgtccg cgtcatacga
      661 cctcccctcc tttgatcacg gtctgcacgt cgcgcagcgc ccggacgtct tcgccggcat
      721 cgcgtgcgag caggagcaag tcggcgggtt cgccgacggc gagcgtgccg ccgtgcaagc
      781 cgatcagctc ggcggcaacg gcggtggcgg cgtggagcgc ctgctgcggc gtcattccga
      841 gcagcgacca catcagctcg acttcgtagg cgtagttctc gtggtagttg tagggcgtgc
      901 cggcgtcgga tccgcccgcg atgcgcaccc cgttctcgta ggcgcggcgg acattggcga
      961 gcatcgcttc gttgacggtg cgcgctttgt cgagaacgtg ttgcggctgc cgcccgtctt
     1021 cgaggtgagc gaggatgcag gtcggggcgg tcagcgtcgg cacgagaaac gcatggcgtg
     1081 acttgaagag ttcgatcgct tcgtcgtcga gcatgtgacc gtgctcgatc gaatcgacgc
     1141 cggcgcgcaa tgcgttcttg atcccttgcg tgccgatcgc gtgcgcggcg acgcgcatcc
     1201 cgtggcggtg cgcttcgtcg acggcggccg caagctcgtc gggcgtgagc tgcgcgttac
     1261 cggggacggc gcctttggtt agcactccgc cggtcgcgat cagcttgatg cagtcggcgc
     1321 ccgcccacag ctgctcgcgc acggccttgc gcgcgtccca cggcgagtcg accgcccgcc
     1381 ccaccggcca gccgtgaccg ccggtcatgc acagcacctc acccgcggcg cgcatccgcg
     1441 gaccgggtat gcgccccgcg tcgattgcat cgcgcaggtc ggccgcgatc cggtgcgagc
     1501 tgccgacgtc gcggatcgtc gtcacgcccg cggcgacgct cttgcgcgcg ttttcgaccg
     1561 cggcgatgag ccgctcgttc ggccgccgcg ccgcgatcgc cgccatcgtg tcgggctcgc
     1621 ccgacatttc gacgtgcgcg tgtgcgttga tcaggccggg cgtcacgcag gcggcttcga
     1681 aatcgcacgc gccgccggcc gcgcgcagtt cggcgatgcg gccgccggcg atggcgagat
     1741 cgacgttgcg tttgggcggc tcgagcgtac cgtcgtagag aattccggcg cgaacgatca
     1801 tcgcggtact ccgttttgag cgcggctgcc atttgcctgg cgccgccgcg ccgggaaagt
     1861 tcgaaccgcg cgcgaagggg gtcgcatcga gatgaagcgt ccgcttccgc tgtggctcgc
     1921 cgccgttctg cccgcgacga tcgccgccca cgcgctggga tacgccgtta cggggcaaag
     1981 catggccgac ggccggcacc tgtggctcgt tccggcgttc gagtcgtcgc tggcggtgct
     2041 gttgctgctc gcaaccgcgc tgatcggcgg cgcgctggtg cgagccggcg tgttcgcccg
     2101 tccgtcggtc gaagctgcgc cgctcgatct atggctgcgg ttgtccgccg cccagacggc
     2161 gcttttcgtg gcgatcgagc gtgccgaagg cacccccgtc gggctgatcg gctgcgcgat
     2221 tcaaatcgcg gtcgcgctcg gcgccgcgct gctgctcgcg gccttcgcgc gcgcgctcgc
     2281 cggctgcgtg cgcaccggcg acgtcgccgc ccggtttctt acgcgcgccg ttgcggcgcc
     2341 cgtttacctc ggccggccgg tttcgcgggc cgacgtcctc gctgccgtag ccggcagccg
     2401 ccgctttcaa cggccgcctc cgccgcgctg aaccgttctt tcaacgcttc ggcactgcgg
     2461 aggtatcgac cgtgctgcga ttacgtgcag taacatttcc cggtatttta ttcgttgcga
     2521 tcctggccgc cggctggtgg ccggcgcgcg cgttggccgt gccggtcttc gccaacggac
     2581 aaggcgtctc gtgtgaaacc tgtcacacga cctttccggg gatgacgcgc tacggcatga
     2641 tggtcatgat gacgaacttt caaatcctca accgccatct gcaagaccag gcgctgccgt
     2701 tggccgcacg gctctacatc acgtcgtatc tagccaatca cagccaaccg ggctcgatcg
     2761 ccgtctccga cctgtcgctg ctcgcagccg gattcatggg gagaaatttt acctattacg
     2821 ccgagcagca cgtcatcgac gacgcgcaaa tcggcgacac cgagcagttg tggctttcgt
     2881 ggaacgggct gctgcacggc accaactcga ttcaggccgg aaaatttcac acgccgtttc
     2941 cgttcatgcc tgcgcacgcc tggacgctgg ccgactatct gctcgcaacc cagacgaccg
     3001 ggcagaacga ttggaatccc aacgacgcgc gctggggctt tgccttcaat ggaatgtcca
     3061 acgagttcat gtacaacgcc gcctatttga ccggcgacgg accgaccagc gaggcgttcg
     3121 actataactc ccagacgcag ccgcgcaccg tcgacctcaa cgtgtcgtac ggcgggatgt
     3181 ccgagccgtg gtcggtgggt ctggtgggca tttgggggca ggcgccgctg cacgacgccg
     3241 ccaccaatct gttcgacgga caggacccgt tctcgcgcga agggctctac ttcggctatc
     3301 agaccgacgc ttggcacctg caaacgatgt actatcacgg tttcgacgct cgtcccgata
     3361 ccggcgcgtt cgacatcccg ctcaacgggt ttttcctcga gatcgagcgc gacttcgggt
     3421 ggcgcaacca cgtagtcgtc cggtacgacg ttgcgtccag cgacacgctc gaccagcaat
     3481 acctgatcga cgtctcgcac aacgtccagc ccaatctcgc gctgatcggc gagctgctcg
     3541 tcgggccgca acacgtcccg cagatcggct ttcaaatcgc ctatgccgga ccgtacgaaa
     3601 tgggcaaacg tttcgtctgg cagccctcgg gcggcagcga ctcgatcgcg aaggggatcg
     3661 cggtcgtacc ggccggcgca aacgccgcac catcgtccct acccgcgccc gcgggaagcg
     3721 gcgcgcaatc tacgggtgcc gacgccaacg acggcgcgcg gctcgtacag gccaacggct
     3781 gcaccggttg tcacggcgcg caatggcaag gcggcgtcgg gccggcgctc ttcggcatcg
     3841 agcaccgcat gtcgccgctg gagatcgcgc tgcacatcaa gagcccggct gcgccgatgc
     3901 cgaacttcgg cttcaccgac gcgcaagtcg ccgacatcgt cgcgtattta tcgtcgctcg
     3961 acggcggcgg cgcgcaggcc gggccggtgg tcacgttcga tccgagtacg ccggtgaaaa
     4021 acgcgacgat ccgcgtcgtc ttcgccggaa cgccgccgca tcgcgtgacc gcgctgccga
     4081 cgatgcagat gggcgccggg aagatgcaga cgcaggcggt aacgctgcag ccgtcgccga
     4141 gcgatccgca taccttcacc ggtacgatcg acttctcgat gggcggaccg tggatcgtga
     4201 tcatcgaata cgacgggacg acgatgagcg ttccgctcaa cgtggggatg taatgcggcg
     4261 ccggacgttc gtcgcggctg cggccggaac catggcggtg gccgctttgc cgaagctgac
     4321 gccgcgggcg ctggccgccg accgcgtcga cgttacgctg accgccgcgc cgctgaactt
     4381 ttcgccgg
//
LOCUS       DASUCJ010000917         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_391740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000917 DASUCJ010000000
VERSION     DASUCJ010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFU90_10940"
     CDS             complement(<1..893)
                     /locus_tag="VFU90_10940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5480342.1"
                     /translation="MPVPLLDLRAQYAQIKDEVVRAMMQVVDDQLFILGQPVERLERA
                     VAALSHTKHAIGCASGTDALLLALRALDIGRGDEVITTPFTFFATAGTIHNVGATPVF
                     VDIDASTFNIATPEAAAAVTGRTRAVVPVDLFGQMSRVEELAAAMPNVPLIEDAAQTI
                     GARRSINGTWVMAGERATIGTFSFFPSKNLGGYGDGGMIVTQDDEIATRLRRLRVHGG
                     ATQYFHDEVGYNSRLDALQAAVLEAKLPHLAEWSSKRREHAAYYDRAFADVPEIRSPT
                     IDAGNESIYNQYTIGADRRDG"
     gene            complement(1019..>1252)
                     /locus_tag="VFU90_10945"
     CDS             complement(1019..>1252)
                     /locus_tag="VFU90_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480343.1"
                     /translation="VNRQVQLLINTPLGKHSQRDDYSMRQAAVAHRVAYTTTLSAASA
                     ACDAILSLRFRPPRVRSLQEWHELVRADAVTAD"
BASE COUNT          201 a          414 c          421 g          216 t
ORIGIN      
        1 aacccgtcgc gccgatccgc gccaatcgtg tactggttgt aaatcgactc gttgcccgcg
       61 tcgatggtcg gggaacggat ttcgggaacg tcggcgaacg cgcgatcata gtacgccgcg
      121 tgctcgcgcc gcttgctgct ccattccgcc aggtgcggga gcttcgcctc gagcaccgcc
      181 gcttgcagcg catcgagacg gctgttgtag ccgacttcgt cgtggaagta ctgcgtggcg
      241 ccgccgtgaa cgcgcaagcg ccgcaggcgc gttgcgatct catcgtcctg cgtcacgatc
      301 attccgccat cgccgtagcc gccgaggttc ttggacggga agaagctgaa cgtgccgatg
      361 gtggcccgct cgcccgccat cacccacgtg ccgttgatgc tgcggcgcgc gccgatcgtc
      421 tgcgccgcgt cctcgatgag cggtacgtta ggcatcgccg cggccagctc ttccacgcgc
      481 gacatctggc cgaacaggtc caccggcacc acggctctcg tccgaccggt gaccgccgcg
      541 gctgcctcgg gcgtcgcaat gttgaacgtc gatgcatcga tgtccacgaa caccggcgtc
      601 gcgcccacgt tgtggatggt gccggccgtc gcgaagaacg tgaacggtgt cgtgatcacc
      661 tcgtcgccgc gaccgatgtc cagcgcgcgc agggccagca gcagcgcgtc ggtcccgctc
      721 gcgcatccga tcgcatgctt ggtgtgcgac agggcggcaa ccgcgcgctc gagacgctcg
      781 accggctgcc cgaggatgaa cagctggtcg tcgacgactt gcatcatggc gcgcacgact
      841 tcgtccttga tctgcgcgta ctgcgcccgg aggtcgagca atgggactgg catgtcggtt
      901 caacaggagg ttcgaggagc gggtttggca ggcgtcgcgc agaaatcggg acgtgccggc
      961 ggcggcccac cgcttcgtcg gacgacgtct aacaacgtgt ggacacccgc gcgcggcgtc
     1021 aatccgccgt cacagcatca gctcgcacga gctcatgcca ttcctgcagg gagcggacgc
     1081 gcggaggacg gaaccgcaac gagagaatcg catcgcacgc agcggaggcc gccgagagcg
     1141 ttgtcgtgta cgctacgcgg tgcgcgacgg cggcctggcg catcgaatag tcgtcgcgct
     1201 gcgaatgttt tccgagcggc gtgttgatca gcagttgcac ctgccggttg ac
//
LOCUS       DASUCJ010000918         1396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_254222, whole genome shotgun sequence.
ACCESSION   DASUCJ010000918 DASUCJ010000000
VERSION     DASUCJ010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254222"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..922)
                     /locus_tag="VFU90_10950"
     CDS             complement(59..922)
                     /locus_tag="VFU90_10950"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5480344.1"
                     /translation="MTQKQLVNEAAAAGTITIGGDMTVNRLGFGAMRVTGKGIWGPPP
                     DRERARDLLRRVIAAGVNFIDTADSYGPYVSEDLIAEALHPYPADLVIATKGGLLRSG
                     PGEWEPDASPVHLREAIEGSLWRLRLERIDLYQLHRPDPKVPFEESVGTLADLQREGK
                     IRHIGLSNVTPEELAAARAIVPIVSVQNRYNAEDRGAEDVLDVCQRDGLAFLPWAPIG
                     GMSPLERKTLRHVAEAHRATHTQIAIAWLLARSPVMLPIPGTSSPEHFAENMAAAHIH
                     LTPEEFKSLSS"
     gene            complement(958..>1396)
                     /locus_tag="VFU90_10955"
     CDS             complement(958..>1396)
                     /locus_tag="VFU90_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5480345.1"
                     /translation="FCLQAPALLDPGELLVCDGLNADGDGARYVALRRSDGAALWHGQ
                     TTWNYERFVDDGPVAIVSDRRVHQMLNENGTTSPDSWLTGVDRRTGRELWRTPLESLA
                     VFTLPAVGDGLVVVGSATFTWADPQVAGSPGVAGLWAWKLPRP"
BASE COUNT          226 a          436 c          498 g          236 t
ORIGIN      
        1 ggatgacaca gggcggtcct tcgactgcgt gaagtgagcg gagctcactt cactccgctc
       61 aggatgacag agatttgaac tcttccgggg tgaggtggat gtgggcggcg gccatgtttt
      121 cggcaaagtg ttcgggcgag ctggtgccgg gaatcggcag catcaccggc gagcgcgcga
      181 gcagccacgc aatcgcaatt tgcgtgtggg tggcgcggtg cgcttcggcg acgtgacgaa
      241 gcgttttgcg ttcgagcggc gacatgccgc cgatcggcgc ccacggcaga aacgctaagc
      301 cgtcgcgctg gcagacgtcg agcacgtctt cggcgccgcg gtcttcggcg ttgtagcggt
      361 tttgcaccga gacgatcggg acgatcgcgc gcgccgccgc gagctcctcg ggcgtgacgt
      421 tcgaaagccc gatgtggcgg attttccctt cgcgctgcaa atcggcgagc gttccgaccg
      481 attcctcaaa cggcaccttg gggtcggggc ggtggagctg atagagatcg atgcgctcca
      541 ggcgcagacg ccacaagctg ccttcgatcg cctcgcgcaa atgcacgggg ctcgcgtcgg
      601 gctcccactc gccggggccg gagcgcagca atccaccttt ggtggcgatc accaaatcgg
      661 ccggatacgg atgaagcgct tcggcgatga gatcttcgct cacgtagggg ccgtaggagt
      721 cggcggtgtc gatgaagttc acgccggcgg cgatcacgcg gcgcagcagg tccctggctc
      781 gttcgcggtc gggtggcggg ccccagatgc ctttgccggt gacgcgcatc gcgccgaacc
      841 cgagccggtt gaccgtcatg tcgccgccga tcgtaatcgt accggcagcg gcagcttcgt
      901 tcacaagctg tttttgcgtc atggctacct cttacccaga atagaacccg gccgggctca
      961 gggacgtggc agtttccagg cccataggcc ggcgacgccg ggcgagccgg cgacctgcgg
     1021 atccgcccag gtaaacgtcg cgctgccgac gaccaccagg ccgtcgccga cggccggcag
     1081 ggtgaagacc gcgagactct cgagcggcgt gcgccagagc tcgcggccgg tgcggcggtc
     1141 gacgccggta agccagctgt cggggctggt ggtgccgttt tcgttgagca tctggtgcac
     1201 gcggcggtcg gaaacgatcg cgaccggacc gtcgtcgacg aaacgctcgt agttccacgt
     1261 cgtttggccg tgccatagcg cggcgccgtc gctgcggcgc agcgcgacgt agcgagcgcc
     1321 gtcaccgtcg gcattgagac cgtcgcagac gaggagctcg ccgggatcga gcagcgccgg
     1381 cgcctgcagg caaaat
//
LOCUS       DASUCJ010000919         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_117042, whole genome shotgun sequence.
ACCESSION   DASUCJ010000919 DASUCJ010000000
VERSION     DASUCJ010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117042"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..617
                     /locus_tag="VFU90_10960"
     CDS             150..617
                     /locus_tag="VFU90_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5480346.1"
                     /translation="MFDTIIVPIDGSNCAEKAFRLAMTLAKAQNAELRVCSVVDPLDY
                     LGSTPPSPLAGQQLEAARGDAERGIADAVSRAKASGLRAEGQLLLGAPPFEIVNYASM
                     PGVQAIVMGTHGRSGLSRLMTGSVAESVLRSAPCPVILLREGVAVPQVEAAVS"
     gene            614..958
                     /locus_tag="VFU90_10965"
     CDS             614..958
                     /locus_tag="VFU90_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480347.1"
                     /translation="MSAREQTVYAVRLIRVANNSFARLYGEIASFMEGPGSEIFGCRE
                     IHLYGNEANSEILIVSEYVSCRDWSHAQWDQRLGELLEEIVVNSETIHFGLYTGHHRF
                     LARHELAAVQTP"
     gene            967..1434
                     /locus_tag="VFU90_10970"
     CDS             967..1434
                     /locus_tag="VFU90_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480348.1"
                     /translation="MSKVFERRAVACPYTLAKGYLAAELSERARTGQPSVLTLRAAVA
                     GADFARAVVVTYAAGSDPMHFDEPWRVHWTPEGGGPYPDFEGELTVRADYDYSSSILE
                     LAGEYQPPGGALGAAFDHTIGSRIASATASDLLAEIGARMEARYHSDELAKRG"
BASE COUNT          271 a          496 c          535 g          253 t
ORIGIN      
        1 atcgacggca cgtacgcgcc cccgcttggc gtcgccggag aacttttcga cgaggcggtc
       61 ggtgaaacga tcgccgagca caccgcccaa acgctgctac gcgatatcgc ccgcgcgatc
      121 tccgcgccgg cggcgatgcg agcgtagcga tgttcgacac catcatcgtc ccgatcgacg
      181 gctcgaactg cgccgaaaaa gcgttccggc tggcgatgac gctcgcgaag gcgcaaaacg
      241 ccgagctgcg cgtctgttcc gtggtcgatc cgctcgacta tctcgggagc acgccgccca
      301 gtccgctggc cggccagcag ctggaggcgg cccgcggcga cgccgaacgc gggatcgccg
      361 acgcggtcag ccgggccaag gccagcggtc ttcgggccga agggcaactg ctgctgggcg
      421 cgccgccgtt cgaaatcgtt aactacgcct cgatgcccgg cgtgcaggcg atcgtcatgg
      481 ggacgcacgg ccggtccggg ctaagccgtt tgatgaccgg aagcgtggcc gaaagcgtat
      541 tgcggtcggc gccatgcccg gtgatcttgc tgcgcgaggg tgtcgccgtg ccgcaggtgg
      601 aggcggccgt atcgtgagcg cgcgcgaaca gacggtgtac gcggttcgct tgattcgggt
      661 ggcgaacaac agtttcgccc gactctacgg cgagatcgcg tcgttcatgg aaggccccgg
      721 gagcgagatt ttcggatgcc gcgagatcca tctctacggc aacgaagcca actcggaaat
      781 tctgattgtg tcggaatacg tctcgtgccg ggattggtcg cacgcgcagt gggatcagcg
      841 gctcggcgag ctgctcgaag agatcgtcgt caactccgaa accattcatt tcggtctata
      901 cacgggccac caccgctttc ttgcacggca cgagttggcc gcggtccaaa cgccatgagg
      961 agtaccatga gcaaagtttt cgagcgacgc gccgtcgcgt gcccgtacac gctggcgaag
     1021 ggttatctcg cggccgagct gtcggagcgc gcccgtacgg gtcagccgag cgtcctcacg
     1081 ctgcgcgccg cggttgccgg tgcggatttc gcacgcgcgg tcgtcgtgac gtacgccgcc
     1141 ggttcggatc cgatgcattt cgacgaaccg tggcgggtgc actggacgcc cgaggggggc
     1201 gggccgtatc cggatttcga gggcgagcta acggtgcgtg ccgactacga ctacagctcg
     1261 tcgatcctcg agttggcagg cgaataccag ccgccgggcg gcgcgctggg agcggccttc
     1321 gatcatacga tcggctcgcg catcgcctcc gccaccgcgt cggatctgct cgccgagatc
     1381 ggcgcgcgga tggaagcccg ctaccacagc gacgagctcg cgaagcgcgg gtaaagagga
     1441 gctaggagag ccatgatgcc gatctttcgc gaccgaagcg acgcggggcg caagctgagc
     1501 gccgagctgg aagactaccg cgagaagccc aacgcaatcg ttctggcact gccgc
//
LOCUS       DASUCJ010000920         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1415991, whole genome shotgun sequence.
ACCESSION   DASUCJ010000920 DASUCJ010000000
VERSION     DASUCJ010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415991"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="VFU90_10975"
     CDS             complement(<1..1883)
                     /locus_tag="VFU90_10975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU5480349.1"
                     /translation="MMSAHPSPAFRLTLRARGSSGIVALAAIAVGITACSRKASPATP
                     ASTTQTAELPPLKVVDPSYMDTTVTACTDFNLYANGGWLERDTIPPAYSESGVFKDMA
                     DRNELVVRSVLDDAMAKAKSLPDTSTQYKLGTFYGSCMDSSAAEAAGVSPIAPQLAAI
                     DSITSTAGLIPEVAALQMEGTNVLFRYGPSVNAHDAEHYIADFDRGGLGMPDRDYYLS
                     AAPSADSMRQAYRAHIAKMLVLGGEDSAAGSKDAERVMSLETEMARAQLPRVARRDPK
                     ATDHQMAFAKLHELAPDVDWTAYLHDVGLSSPVSLVNVGEPAYYKALDALVAKRPVED
                     WRAYLRYHVLSSAAPWLSSAFVNENFAFASRFSGAKELLPRWKRCLRVTDAQLGEALG
                     QAYVARTFPPDAKAKAKAVIDDIRAAFHDRLLRLTWMSDSTRAHALDKLAKMGEKVGY
                     PDRWRDYSKLRVSTGPFVLDDQNASRFEWERVAQRPGQPVDKTEWGITVPTVNAYYNP
                     SKNEMVFPAGALVPQTFDPKADDGANYGSLGASWAGHEMTHGFDDEGRHYDAQGNLTD
                     WWTPADSKRFNAQAELDAKQYDGYIQVDTFHVNGHLTLGENIADYGGALTAYDALEKA
                     LQR"
     gene            complement(1880..>2364)
                     /locus_tag="VFU90_10980"
     CDS             complement(1880..>2364)
                     /locus_tag="VFU90_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5480350.1"
                     /translation="TFWIDTQTDRFKAISPGAGLPDLYSMYSETDIHRYLRLFFNMKA
                     PWDNVQEYWDHSPMKYVNNVKTPTMILHGQADTRVPIPQAEEFYRALTERHVPVEFVT
                     YPRENHGFVEPRHIEDRYQRYLVFFGKYLNNPPVTEPRDVVERMSKDLPAQQVATTKP
                     "
BASE COUNT          371 a          797 c          761 g          435 t
ORIGIN      
        1 tcgcgctgga gcgccttctc tagcgcatcg taggccgtga gcgcgccgcc gtagtcggcg
       61 atgttctcgc ctaacgtcag gtggccgttc acgtggaacg tatcgacctg gatgtagccg
      121 tcgtactgct tcgcatccag ctcggcctgc gcgttgaacc gcttcgagtc cgccggcgtc
      181 caccaatcag tgagattgcc ctgggcgtcg tagtggcggc cctcgtcgtc gaagccgtgc
      241 gtcatctcgt ggcccgccca gctcgcgcct aacgaaccgt agttggcgcc atcgtcggcc
      301 ttcgggtcga acgtctgcgg caccagcgcg ccggccggga acaccatctc gttcttgctc
      361 ggattgtagt acgcgttcac ggtcggcacc gtgatgcccc actccgtctt gtccaccggc
      421 tggcccggcc gctgcgccac gcgctcccac tcgaagcgcg acgcgttctg gtcatcgagg
      481 acgaacggtc ccgtcgacac ccgcagcttc gagtagtcgc gccacctgtc gggatagccg
      541 accttctcgc ccatcttcgc cagcttgtcc agcgcgtgtg cgcgcgtcga atcggacatc
      601 caggtgagcc gcaacagccg atcgtggaat gcggcgcgga tgtcgtcgat caccgccttc
      661 gccttggcct tcgcgtcggg cgggaacgtc cgggccacgt acgcctggcc taacgcttcg
      721 cccaactgcg catcggtcac gcgcaggcac cgcttccatc gcggcaacag ctccttggcg
      781 ccgctgaacc gcgaggcgaa cgcaaaattc tcgttcacga acgccgacga gagccacggc
      841 gcggcgctcg acagcacgtg gtagcgcagg taggcgcgcc aatcttcgac gggccgcttc
      901 gccacgagcg cgtcgagcgc cttgtagtac gccggctcgc ccacgttcac gagcgacacg
      961 ggcgacgaga gccccacgtc gtgcagatac gccgtccaat cgacatccgg cgccagctcg
     1021 tgcagcttcg caaacgccat ctgatggtcg gtggccttgg gatcgcggcg cgcgacgcgc
     1081 ggcagctgcg ccctggccat ctccgtctcg agcgacatca cgcgctcggc gtccttcgac
     1141 cccgccgcgc tgtcctcgcc gcccaacacc agcatcttcg cgatgtgcgc gcggtacgcc
     1201 tggcgcatcg agtcggccga cggcgcggcg ctcaggtagt agtcgcggtc gggcatgcct
     1261 aacccgccgc ggtcgaagtc ggcgatgtag tgctccgcat cgtgcgcgtt caccgacggt
     1321 ccgtagcgga acaggacgtt cgtgccttcc atctgcagcg cggccacttc cgggatcagg
     1381 cccgccgtcg atgtgatcga atcgatcgcc gccagctgcg gggcgatcgg cgacacgccg
     1441 gccgcctcgg ccgccgacga atccatgcag ctgccgtaga acgtgcctaa cttgtactgc
     1501 gtgctcgtgt ccggcagcga cttggctttc gccatcgcgt cgtcgagcac ggatcgcacg
     1561 acgagctcgt tacggtcggc catgtccttg aacacgcccg actccgagta cgccggcgga
     1621 atcgtatcgc gctcgagcca gccgccgttg gcgtacaggt tgaaatccgt gcacgccgtg
     1681 accgtcgtat ccatgtacga tggatcgacc accttgagcg gcgggagctc ggccgtctgc
     1741 gtcgtggagg ccggtgtggc cggcgaggcc ttccgcgagc aggccgtgat accgacggcg
     1801 atcgcggcca gggcgactat gcctgacgat ccgcgcgcgc ggagcgttag gcggaaagca
     1861 ggcgatggat gggccgacat catggtttgg tggtggcaac ctgttgtgcg gggaggtcct
     1921 tgctcatccg ctcgaccaca tcgcgcggct cggtgaccgg cggattgttg aggtatttgc
     1981 cgaagaacac cagataccgc tgatagcggt cctcgatgtg ccgcggttcg acgaagccgt
     2041 ggttctcgcg cggataggtg acgaattcca cgggcacgtg acgctcggtc aacgcccgat
     2101 agaactcttc ggcctgcgga atcggcacgc gtgtgtccgc ctggccgtgc agaatcatcg
     2161 tcggcgtctt cacgttgttc acgtacttca tcggcgagtg gtcccagtat tcctggacgt
     2221 tgtcccacgg cgccttcatg ttgaagaaga gccgcagata ccgatggatg tcggtctccg
     2281 aatacatcga atagagatcg ggcagtccgg cgcccggcga gatcgccttg aagcggtcgg
     2341 tctgggtgtc gatccagaac gtca
//
LOCUS       DASUCJ010000921         6274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1155759, whole genome shotgun sequence.
ACCESSION   DASUCJ010000921 DASUCJ010000000
VERSION     DASUCJ010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155759"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFU90_10985"
     CDS             complement(<1..149)
                     /locus_tag="VFU90_10985"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5480351.1"
                     /translation="MAADDSTLKRCTWATTAESIRYHDEEWGVPVRDDRRLFEFLVLE
                     GAQAG"
     gene            228..1142
                     /locus_tag="VFU90_10990"
     CDS             228..1142
                     /locus_tag="VFU90_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5480352.1"
                     /translation="MIEPPAARPAQTYDEALQRARAYAALDDESILPAARTVLLEHGE
                     RTPLAVVLLHGFTNNPAQYAEFAPLLHRAGVNVFVPRMPKHGERDRLTKTIASLTAGE
                     LLSSAGEALDIAFGLGERAGVLGISMGGSMSAYFAQYRPVSIAVPVAPDFALLQLPYG
                     VSRAFARVFSMLPNFFAWWDPRDRAQHRPATAYPRFSTRALMETLRIGDDVFAAARRG
                     PQQAERIVTVVNGCDPAVNNEVTREVSSAWSGWRPDGAGYVELRGLPENHDIVDPANP
                     LARTGLVYPHLLAALGVPAAGERNEGSA"
     gene            1139..2500
                     /locus_tag="VFU90_10995"
     CDS             1139..2500
                     /locus_tag="VFU90_10995"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480353.1"
                     /translation="MTGRLHRLLTILALGLAAGSASACSSAPRGGFGGSPLPPAASLP
                     FALPDGAGKIRHVVIVVQENRSFDNLFQGYPGADTVSHGKDSHGHAIALRPVSLAKQY
                     VIDHSATAMFAACDGTGSVPGTDCRMDGFDLEQAVGGPPHPQYAYVPHRESKPYFDMA
                     HEWVLADRMFQSQIDESFVSHQYIIAAQAQSSVDIPFLPEWGCGGGKGNFVQTIAPGR
                     AFGKTQRPCFDYRTLGDELDAANLSWRFYTSQIADPADGVWSGYQAVRHIRFGPDWKK
                     DVITPQKRFLTDVARGNLANVTWITPTCEESDHVNCGGGFGPSWVSSIVNAVGESKFW
                     KTSAIFVMWDDWGGLYDHVPPPHVGDDGLGFRVPLLVISPYAKHDYVSRVRYEHGSIL
                     KFAENVFGLGRLAESDTRANSPAGDCFDFTKPPRAFVPIRAPKGRSFFIDRPVDERPP
                     DYE"
     gene            complement(2502..4013)
                     /locus_tag="VFU90_11000"
     CDS             complement(2502..4013)
                     /locus_tag="VFU90_11000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5480354.1"
                     /translation="MRNERWPLAVAIVVLIVHLIGNPHYGFFRDELYFIICGFHPAWG
                     YVDQPPVVPLLAAGSQLFGHSLFLLRAVAALFAAAGAYVTCLLAIELGGDAFAQVLAG
                     LAYLFAGVLMSFGMKVGPDMVGLWLWPLTALYVLRIAKGGDPRQWLFAGTAIGFSLES
                     KYSAAFFAAALIGGLLLTPQRRILGSRWFAGGALLAAGIALPNFLWQALHDFPMLELL
                     RNGQNGKNVVVGPALYLFQQLLLTNLFLSPIWIVGLIALLRDRVARFLGYAYVLLIAM
                     MIALHGKHYYPGDVYPILMAAGGVTFSAWTRRSAALRAALVAAAIAAGLFFLPFSLPV
                     LAERPMVEYQAFVSRVLHLSRSAMATERHRTALLPPDWADMHGWPELAALVARIYYAL
                     PPEQRAQAAIVASNYGEAAAIDFFGKAYGLPPAISGHNNYWLWGTHGASGNVVIDVNG
                     DCGAARKLFRTTRLAARFDPPWVISYEQDIPIMVCTGPTQNLTALWPSLKNYI"
     gene            complement(4028..5530)
                     /locus_tag="VFU90_11005"
     CDS             complement(4028..5530)
                     /locus_tag="VFU90_11005"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5480355.1"
                     /translation="MKRRFVVEPAPLARLLCDLAAFTIPAIAYVAGASHEPGSWDTAE
                     LQGVPYILGITHPTGFPFYVLLGYAWSHAVVLGTIAFRMNVMSGVAIAAACSAAYAAA
                     LELGAWRPVALAATLWYAFAPDVLSHATRAEAQDLAVACEAFAVYAFLRWMRGADPRW
                     FAGAAALIGLGIAAHPNALWLIPGTIVGALATRRRPSLRLAIASAGLLLAGLLLYAYL
                     PLRSAYVVAHGLDPTAVLPGTDGGIFWNYNNPSTPQGLLRALSGTESEAPHYLLASLN
                     PVHVQDALWAFVTGIARQFGAFALILVTIGAVNAWRRDWRTTLFLCVACGAALLFSVT
                     YSNEADVGRYRLMALWLAVPLLGAVAPGAPRGDALAAFGRLALGVFMAAAAGASLYGQ
                     RGAFVHAPAEGGRWVIDAVRPFVPPGGVIVVDGWLDATSLAYGAYADGSLPGRIIVSG
                     WHPELAGEYSVWAKRRPVFVLADPHDVTDVPGSNPPVRLDPYHELFPISP"
     gene            complement(5527..>6274)
                     /locus_tag="VFU90_11010"
     CDS             complement(5527..>6274)
                     /locus_tag="VFU90_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5480356.1"
                     /translation="SVTLRSRKDDERTVLVKALSSEAALRYRAWVEGNRRLVHERTDG
                     RVGYLHIPDMGPWGFSEFHRGFLTEFDRQGLIVDVRYNRGGHVSSLLLEKLARKRVGY
                     DAPRYGSLVPYPPESVGGPIVAMTNQFAGSDGDIFSHCFKLYSLGPLVGKRTWGGVVG
                     IEPYHHLVDGTLTTQPEFSFWFVDAGWGVENYGTDPDYDVDVAPHDYRDGKDPQLDLA
                     LELMERELAGYERKLPDLSNRPSLPLPTLS"
BASE COUNT         1147 a         2072 c         2105 g          950 t
ORIGIN      
        1 aagccggcct gcgcgccctc gagcacgagg aattcgaaca gcctgcgatc gtcgcgaacc
       61 ggcacgcccc actcttcgtc gtgatagcgg atcgactcgg cggtggtcgc ccacgtgcag
      121 cgtttcaggg tcgaatcgtc ggcagccatc ggacgaacgg tgtagaggac ggttgggtgc
      181 gaatcctgcc ggaaggaccg ccaattcgcc ggcggtaacc agtgcgggtg atcgaaccgc
      241 ccgccgcccg gcccgcgcag acgtacgacg aagcgctgca gcgcgcgcgc gcgtacgccg
      301 cgctcgacga cgagtccatc ttaccggccg cgcgcacggt gctgctcgag cacggcgaac
      361 gcacgccgct ggcggtcgtt ctgctgcacg ggttcaccaa caatccggcg cagtacgccg
      421 agttcgctcc gctcctgcat cgcgccggcg tcaacgtgtt cgtgccccgc atgcccaagc
      481 acggcgaacg cgaccgcctg accaaaacga tcgcttcgct gaccgccggc gaactcttga
      541 gcagtgccgg cgaggccctc gacatcgcgt tcggcctggg cgaacgggcc ggggtgctcg
      601 gcatttcgat gggcggatcc atgagcgcgt acttcgcgca gtaccggccg gtctcgatcg
      661 ccgtgccggt tgcgccggac tttgccttgc tgcagctgcc gtacggcgta agccgcgcgt
      721 tcgcgcgcgt gttttcgatg ctgccgaact tctttgcgtg gtgggatccg cgcgatcgcg
      781 cgcagcatcg tccggcgacc gcttacccgc gcttttcgac gcgcgcgctg atggagacgc
      841 tgcggatcgg cgacgacgtg ttcgccgccg cgcgccgcgg tccgcagcag gccgagcgaa
      901 tcgttaccgt cgtaaacggg tgcgacccgg cggtcaacaa cgaagtgacg cgcgaggtat
      961 cgtcggcctg gagcggctgg cggccggacg gcgccggcta cgtcgagctg cggggtctgc
     1021 cggaaaatca cgacatcgtc gacccggcca atccgctggc ccgcaccggt ttggtgtacc
     1081 cgcacttgct ggcggcgctg ggggtgccgg cggccggcga acgaaacgag gggagcgcgt
     1141 gaccggccgt cttcatcgcc tgctaaccat cctcgcgctg gggctcgcgg ccggctcggc
     1201 gtccgcgtgc tcaagcgcgc cgcgcggagg gttcggcggt tcgccgttgc ccccggcagc
     1261 gtcgctcccg ttcgcgctgc cggatggtgc cggaaagatc cgccacgtcg tgatcgtcgt
     1321 tcaggagaac cgcagcttcg acaacctttt tcagggatat ccgggcgccg acacggtttc
     1381 gcacggaaaa gattcgcacg ggcacgcgat cgcattgcgg ccggtgagcc tggcgaaaca
     1441 atacgtgatc gaccattcgg cgacggcgat gttcgccgct tgcgacggaa ccggctcggt
     1501 tcccggtacc gactgccgga tggacggctt cgatctcgag caggccgtgg gcggtccgcc
     1561 gcatccgcag tacgcgtacg tcccccaccg cgaatccaag ccgtacttcg acatggcgca
     1621 cgaatgggtg cttgccgacc ggatgttcca atcgcaaatc gacgaaagct tcgtttcgca
     1681 tcagtacatc atcgcagctc aggcgcagtc gagcgtcgac attccgttcc tccccgaatg
     1741 gggatgcggc ggcggaaaag gaaatttcgt tcagacgatc gcgccgggac gcgccttcgg
     1801 aaaaacgcag cgcccgtgct tcgactaccg caccttgggc gacgagctcg acgccgcgaa
     1861 tttgagctgg cgcttctata ccagccagat cgccgatccc gccgacggcg tgtggtcggg
     1921 ttaccaagcc gtgcgccaca tccggttcgg ccccgactgg aaaaaggacg taatcactcc
     1981 gcaaaaacgc tttttgaccg acgtcgcgcg gggcaatctg gcgaacgtga cctggatcac
     2041 gccgacctgc gaggagtccg accacgtgaa ctgcggcggc ggcttcgggc cgtcgtgggt
     2101 gagctcgatc gtcaacgcgg tcggcgagtc gaagttctgg aagacctcgg cgatcttcgt
     2161 gatgtgggac gattggggcg gcttgtacga tcacgtgccg ccgccgcacg tcggcgacga
     2221 cggtttgggg tttcgcgtcc cgctgctcgt catctcgccg tacgctaagc atgactacgt
     2281 ctcgcgcgta cgctacgagc acggaagcat cttgaagttc gccgagaacg tcttcgggct
     2341 cgggcggctg gccgagagcg atacgcgcgc caattcgccg gccggcgact gcttcgattt
     2401 tacgaagccg ccgcgcgcct tcgtgccgat ccgtgcaccg aaaggccgct cgttcttcat
     2461 cgaccgtccg gtcgacgaac gtccccccga ctacgaataa gctaaatgta attcttgagc
     2521 gagggccaga gcgccgtcag gttttgcgtg gggccggtgc agaccatgat cgggatgtcc
     2581 tgctcgtacg agatcaccca cggcggatcg aaacgcgcgg ccagccgcgt agtgcggaag
     2641 agtttgcggg cggcgccgca gtcgccgttg acgtcgatca cgacgttgcc cgaagcgccg
     2701 tgcgttcccc acagccagta gttgttgtgc cccgaaatcg ccggcggcag cccgtaagcc
     2761 ttgccgaaga aatcgattgc ggcggcctcg ccgtagttgc ttgcgacgat cgcggcttgc
     2821 gcgcgctgtt cgggcgggag agcgtagtag attcgtgcga ccagcgcggc cagctcgggc
     2881 cagccgtgca tatcggccca gtcgggcgga agcagcgcgg tgcggtgccg ctcggtggcc
     2941 atcgccgagc ggctgaggtg cagcacccgc gagacgaagg cctggtactc gaccatcggc
     3001 cgttcggcga gaaccggcag cgaaaacggc aggaagaaga gcccggccgc gattgcggcg
     3061 gccaccagcg ccgcgcgcag cgccgccgag cgtctcgtcc aggcggaaaa cgtcacgccg
     3121 ccggccgcca tcaggatcgg atagacgtca cccgggtagt agtgctttcc gtgcaacgca
     3181 atcatcatcg cgatcagcag gacgtaggcg tacccaagaa accgcgcgac gcggtcgcgc
     3241 agcagcgcga tcagcccgac gatccagatt ggcgaaagaa agagattcgt gagcagcagc
     3301 tgctggaaga gatagagtgc cggtccgacg accacgttct tgccgttttg tccgttacgc
     3361 agcagctcga gcatcggaaa atcgtggagc gcttgccaca ggaagttcgg cagcgcgatg
     3421 ccggcggcga gcagcgcgcc gccggcgaac cagcgcgatc cgaggatgcg ccgctgcggc
     3481 gtcagcagca aaccgccgat cagcgcagcg gcgaagaagg cggcgctata tttgctctcc
     3541 agcgaaaagc cgatcgcggt gccggcgaac agccattgcc gcggatcgcc gcctttggcg
     3601 atccgcagta cgtaaagcgc cgtcagcggc cacagccaca ggcccaccat gtcgggcccg
     3661 accttcatcc cgaagctcat gagaacgccg gcgaacagat acgccagtcc ggccagaact
     3721 tgcgcgaagg cgtcgccgcc cagttcgatt gcgagcaaac atgtgacgta cgctccggcg
     3781 gcggcaaaca gcgccgctac cgcgcgcaac aggaaaagcg aatgtccgaa cagctgcgag
     3841 ccggccgcga gcagcggcac gaccggcggc tgatcgacgt agccccacgc ggggtggaat
     3901 ccgcagatga tgaagtacag ctcgtcgcgg aagaacccgt agtgcggatt gccgatcagg
     3961 tgcacgatca gcacgacgat cgcaacggcg agaggccagc gctcgttcct cataccgagc
     4021 gctcgttcta cggcgaaatc gggaacaact cgtggtaggg gtcgagccgc accggcggat
     4081 tcgagcccgg gacgtccgtg acgtcgtgcg gatccgccag cacgaatacc ggacgccgct
     4141 tcgcccaaac ggaatactcg ccggccagtt cggggtgcca acccgaaacg atgattctcc
     4201 cgggcagcga gccgtcggcg tacgcaccgt atgcaagcga cgtcgcatcg agccatccgt
     4261 cgacgacgat gacgccgccc ggcgggacga acggacgcac cgcgtcgatc acccagcgcc
     4321 cgccctcggc gggagcgtgt acgaacgccc cgcgctgccc gtagagcgac gcgccggcgg
     4381 ccgccgccat gaagacgccg agcgcgagac gcccgaatgc cgcaagcgcg tcgccgcgcg
     4441 gcgcacccgg cgcaaccgct cccaacagcg gaaccgcgag ccacaacgcc attaaacgat
     4501 agcgtccgac gtcggcctcg ttcgagtagg tcaccgaaaa cagcaacgcg gcgccgcaag
     4561 cgacgcacag aaacagcgtc gtgcgccagt cgcgccgcca cgcgttgacg gcgccgatcg
     4621 tcaccaatat cagggcgaag gcgccgaatt ggcgggcgat tccggtcacg aaagcccaca
     4681 gcgcatcctg cacgtgcacg gggttgaggg atgcgagcag atagtgcggc gcttccgatt
     4741 cggttcccga aagcgcgcgg agcagccctt gcggcgtgct cggattgtta taattccaaa
     4801 agatgccgcc gtcggtcccg ggaagcacgg cggtaggatc gagaccgtgc gcgacgacgt
     4861 acgccgagcg caacggtaag tacgcgtaga gcagcagtcc cgccaagagc agtcccgccg
     4921 aagcgattgc caaccgcagc gacgggcggc gccgtgtcgc caacgcgcct acgatcgtgc
     4981 cgggaatcag ccagagcgcg ttgggatggg cggcgatgcc cagcccgatc agcgccgcgg
     5041 cgccggcgaa ccagcgcgga tccgcgccgc gcatccaacg caggaaggcg tacaccgcga
     5101 aggcttcgca ggcgaccgcc agatcttgcg cctccgcgcg ggttgcgtgc gacaagacgt
     5161 cgggtgcgaa ggcgtaccac agcgtcgccg cgagcgcgac cggacgccac gcgcccagtt
     5221 cgagagcggc ggcgtaagct gccgaacacg cggcggcgat cgcgaccccg ctcatcacgt
     5281 tcatgcgaaa cgcgatcgtt cccagcacga cggcgtgcga ccacgcgtag ccgagcaaca
     5341 cgtagaacgg gaatccggtc ggatgggtga tccccagaat gtacggcacg ccttgcagct
     5401 cggcggtgtc ccaagatccg ggttcgtggg atgcgccggc gacgtaggcg atcgccggaa
     5461 tcgtaaatgc agccaggtcg cagagcaggc gcgcgagcgg cgccggctct acgacgaagc
     5521 gccgtttcac gaaagcgtcg gcagcggtag cgacggccgg ttcgaaagat cgggaagctt
     5581 gcgttcgtac cccgcaagct cgcgctccat gagctctagt gcgaggtcga gctgcgggtc
     5641 ctttccgtcg cgatagtcgt ggggtgcgac gtccacgtcg tagtcgggat cggtgccgta
     5701 gttttcgaca ccccagccgg cgtcgacgaa ccaaaacgaa aactccggct gagtggtgag
     5761 cgttccgtcg acgagatgat ggtacggctc gatgccgacg acgccgcccc acgtccgttt
     5821 gccgacgagc ggcccgagcg agtacagctt gaagcagtgg ctgaagatgt cgccgtctga
     5881 gccggcgaat tgattggtca tcgccacgat cgggccgccg accgactcgg gcggatacgg
     5941 cacgagggat ccgtatcgcg gagcgtcgta gccgacgcgc ttgcgcgcga gcttttccag
     6001 cagcagcgac gaaacgtggc cgccccggtt gtagcgcacg tcgacgatca gcccttggcg
     6061 gtcgaactcg gtgaggaatc cccgatgaaa ttccgagaaa ccccacggcc ccatgtcggg
     6121 gatgtgcagg tagccgacgc ggccgtcggt gcgctcgtgg acgagccgcc ggttcccttc
     6181 gacccacgcg cgatatcgca aagcggcctc gctcgacagc gccttcacca gcaccgtgcg
     6241 ctcgtcgtcc ttgcgcgatc gcagggtgac ggag
//
LOCUS       DASUCJ010000922         1447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1385501, whole genome shotgun sequence.
ACCESSION   DASUCJ010000922 DASUCJ010000000
VERSION     DASUCJ010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385501"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(128..212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(317..1168)
                     /locus_tag="VFU90_11015"
     CDS             complement(317..1168)
                     /locus_tag="VFU90_11015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5480357.1"
                     /translation="MNPFRSIPRGAYVLGGIALAVVAASGVLLYSTLQVRYRRDISRI
                     VFNRNWDLLDQVRRDAGVTADSLRRAMAEASPGPDADKPYIVVSISDRRLWYKLGDSV
                     FYTTRVAVGSGKTLVKQDGRDEYKFDTPRGRLTVQSKDVGPVWVPPDWHFLEIAEKRK
                     LGVVMLKRGQKVPAADGAVITVDGNDVVKKYPDGHEVPYESGEGREIVDGKNIIVPPY
                     GTNQRKYRGTLGGHRLDLGDGYALHGTDEPKSVGEATSHGCVRLRNEDIDYLYGIVPV
                     GTPVYIY"
     gene            complement(1199..>1447)
                     /locus_tag="VFU90_11020"
     CDS             complement(1199..>1447)
                     /locus_tag="VFU90_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480358.1"
                     /translation="QVPEWVYTDRGLTSPPAARRTLTKALGPVAIVLDGGTVIYSLPT
                     NGPLNDSSYTMPGSVRARSEDLEAIVPNVTKGMVVYFY"
BASE COUNT          244 a          521 c          431 g          251 t
ORIGIN      
        1 caacgcgcgc cgcgtcagcg cgcggtgccg cgcctgcggc cgctacgagt gcgactcctg
       61 caccgacgac tcgaacggcg cgcgcgtagc gatgcgcgcc tgtatgtgca cggaccatcg
      121 gcgcctcccc ggcaaccaga ctgacggcaa cgacggggcc gtctcttggc tttgcgcgtc
      181 ggctcttcgc agccgacttg cccttggcaa ggagcccccg tcacgacggg ggaggaactt
      241 cccataacct taggggggac gcacttcgcg ggaacccgca acccgtgtct acaagccaac
      301 ttgactgacc aaacggtcaa taaatgtaca cgggcgttcc gaccggaacg atgccgtaca
      361 ggtaatcgat gtcttcattg cgcaagcgca cacacccgtg gctcgtcgct tcgccgacag
      421 acttgggctc atcggtgccg tgcagggcgt agccgtcgcc aaggtccagc cgatggccgc
      481 ctaacgtgcc gcggtattta cgctgattcg ttccgtaagg cggcacgatg atgttcttgc
      541 cgtcgacgat ttcgcggccc tcgccggatt cgtacggcac ttcgtgtccg tccggatatt
      601 tcttcacgac gtcgttgccg tccacggtga tcaccgcgcc gtcggccgcc ggcaccttct
      661 gcccgcgctt gagcatcacg acgccgagct ttcgcttctc ggcgatctcg agaaagtgcc
      721 aatcgggcgg cacccacacc gggcccacat ccttgctctg caccgtgagg cggccgcgcg
      781 gcgtgtcgaa cttgtattcg tcgcgcccgt cctgcttcac cagcgtcttg ccgctgccca
      841 cggcgacgcg cgtcgtgtag aacacgctgt cgcctaactt gtaccagagc cggcggtcgg
      901 agatgctgac cacgatgtat ggcttgtccg catccgggcc gggactcgcc tccgccatcg
      961 cccggcgcag cgagtcggcc gtcacgccgg catcgcgccg cacctggtcg agcagatccc
     1021 agttgcggtt gaacacgatc cggctgatgt cacgccggta ccgcacctgc agcgtcgaat
     1081 acagcaacac gccggacgcc gccacgaccg ccaacgcgat gccgcccagc acgtacgcac
     1141 cgcgtggaat cgagcgaaac ggattcatcg cccgatcctc gcggcgccca acatgccctc
     1201 agtagaaata gaccaccatc cctttggtta cgttaggcac gatcgcctcc aggtcctccg
     1261 atcgcgcacg cacgctgccg ggcatcgtgt acgacgaatc gttgaggggc ccgttggtcg
     1321 gcagcgagta gatcaccgtg ccgccgtcca gcacgatcgc cacggggccc aacgctttcg
     1381 tcagcgtccg ccgcgccgcc ggcggcgacg tgagcccgcg atccgtatac acccactccg
     1441 gcacctg
//
LOCUS       DASUCJ010000923         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_911579, whole genome shotgun sequence.
ACCESSION   DASUCJ010000923 DASUCJ010000000
VERSION     DASUCJ010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911579"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFU90_11025"
     CDS             complement(<1..682)
                     /locus_tag="VFU90_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5480359.1"
                     /translation="MNFQQLRFVREAVRQNMNLTEVANVLYTSQSGVSKQIKDLEDEL
                     GVDIFIRRGKRLTGLTEPGKAVHQLIERMLLDAENLRRVARQYADQDNGHLVVATTHT
                     QARYALPKVVRQFTSVFPKVHLALRQGNPQQIAQMIINGEADIGISTEALDRYPDIVT
                     FPCYSWHHVVVVPKGHPLVGRENITLEEIAEYPIVTYDQDFTGRSHIDQAFAQAGAVP
                     DVVLTAIDA"
     gene            complement(759..>1006)
                     /locus_tag="VFU90_11030"
     CDS             complement(759..>1006)
                     /locus_tag="VFU90_11030"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEU5480360.1"
                     /translation="FAYVRPHDLVLYPQASGHRDGIVVGVRRVVTLGGSVRVELEGHE
                     GRVLEAELDRETWRELQLAIGDGVTAVPRSLRVFPAH"
BASE COUNT          181 a          332 c          305 g          188 t
ORIGIN      
        1 ccgcgtcgat cgcggtcagc acgacgtcgg gcacggcgcc ggcctgcgcg aacgcctggt
       61 cgatgtgcga gcggcccgtg aagtcctggt cgtacgtgac gatcgggtat tcggcgatct
      121 cttccagcgt gatgttctcg cgacccacga gcggatgccc cttcggcacg acgaccacgt
      181 gatgccacga atagcacggg aacgtcacga tgtcgggata acggtcgagc gcttccgtcg
      241 agatgccgat gtcggcctcg ccgttgatga tcatctgcgc gatctgctgc gggttgccct
      301 ggcgaagcgc gagatgcacc ttcggaaaaa ccgaagtgaa ctggcgcacc accttcggca
      361 gcgcgtatcg cgcctgagtg tgcgtggtcg cgacgacgag gtggccgttg tcctgatccg
      421 catactggcg cgcgacgcgg cgcaggttct ccgcgtcgag caacatgcgt tcgatcagct
      481 gatgcacagc cttgcccggc tccgtcagcc ccgtcagacg cttgccgcgt cgaatgaaaa
      541 tatcgacgcc cagttcgtcc tcgagatcct tgatctgctt cgagacgccc gactgcgacg
      601 tgtacagcac gttcgcgact tccgtcaggt tcatgttctg ccgcacggct tcgcgcacga
      661 agcgcaactg ctggaaattc atatcgatgt ctccggttcg gccagagtaa gtttgttgtt
      721 caatgcctgg gttgacgcga cgtcgctcag tccatcactc aatgcgcggg gaacacacgc
      781 aacgaacgcg gcacggccgt cacgccgtcg ccgatcgcaa gttgcaactc gcgccatgtt
      841 tcgcgatcga gttccgcctc gagcacacgg ccttcgtgcc cttccagttc gacacgcacc
      901 gagccgccca gcgtcaccac acgccgcacg ccgacgacga tgccgtcgcg atgtcccgac
      961 gcctgcggat acagcacgag atcgtgcgga cgcacatagg cgaacg
//
LOCUS       DASUCJ010000924         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_926933, whole genome shotgun sequence.
ACCESSION   DASUCJ010000924 DASUCJ010000000
VERSION     DASUCJ010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926933"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..960)
                     /gene="nadE"
                     /locus_tag="VFU90_11035"
     CDS             complement(151..960)
                     /gene="nadE"
                     /locus_tag="VFU90_11035"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEU5480361.1"
                     /translation="MRGIADYVGKNRFPGVILGLSGGIDSALTLALAVDALGADKVTA
                     VRLPSRYTSGLSNDLAAEQAQALDVEMLTIPIGAPFAGFLDALAPAFAGRGPDVTEEN
                     LQSRCRGAILMALSNKTGRMLLTTGNKSEYATGYATIYGDMCGGYAPLKDCYKELVYA
                     LARWRNRRGEVVPQAVIDRAPSAELRENQTDQDSLPPYEILDAILQRFVESEQSQAEI
                     VAAGFEEATVCRVARLVLASEYKRRQAAPGPTISTRAFGRDRRYPISSGWK"
BASE COUNT          177 a          352 c          369 g          193 t
ORIGIN      
        1 ccgtttagag gttgattggg aaaagtgccc ggccgtattg gtcttcggct acggagtcgc
       61 acctccacaa ccctcaatcc cttgctgcac aagggattcg cccccttgac tcaagggggc
      121 tgaagcggac tttgctacca atcgccggag ttatttccaa cccgaactaa tcggatagcg
      181 tcggtcgcgg ccgaacgcgc gggtggaaat cgtcggaccc ggcgcggcct ggcggcgctt
      241 gtattcgctg gccagcacca ggcgcgcgac gcggcacacg gtggcctctt cgaatccggc
      301 ggcgacgatt tccgcctgtg actgctcgga ttcgacgaag cgttgcagga tcgcgtcgag
      361 gatttcgtag ggcggcagcg aatcctggtc ggtctggttc tcgcgcagtt ccgccgacgg
      421 cgcacggtcg atcacggcct gcgggacgac ctcaccacga cggttgcgcc agcgcgccag
      481 cgcgtagacc agttccttgt agcaatcctt gagcggcgcg tagccgccgc acatgtcgcc
      541 gtagatggtc gcgtagccgg tggcgtattc actcttgttg ccggtcgtga gcaacatgcg
      601 cccggtcttg ttcgacagcg ccatcaggat cgctccgcgg cagcgcgatt gcaggttttc
      661 ctcggtgacg tcggggccac ggccggcaaa cgcgggcgcg agcgcgtcga ggaacccggc
      721 gaacggcgcg ccgatgggga tcgtgagcat ctccacgtcc agcgcctgcg cctgttcggc
      781 agcaaggtcg ttgctcaggc cggatgtgta gcgcgacggc agtcgcaccg cggttacctt
      841 gtcggcgccg agcgcatcga cggcgagggc taacgtgagc gccgaatcga tgccgcccga
      901 tagcccgagg atcacgcccg ggaaacgatt cttgccgaca tagtcggcaa tgccgcgcac
      961 gatcgccgcg tacgcgcgcg cctcgcgcga ggtgtcggcc tcgaccggcc aatcgatcgc
     1021 ctggaacttg cgcgcggcgt tgtcgtactc ggcgagcaac agcgcgtcga cgaatgccgg
     1081 cgcgcgtgcg g
//
LOCUS       DASUCJ010000925         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_284839, whole genome shotgun sequence.
ACCESSION   DASUCJ010000925 DASUCJ010000000
VERSION     DASUCJ010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284839"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFU90_11040"
     CDS             complement(<1..417)
                     /locus_tag="VFU90_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480362.1"
                     /translation="MDVPASSNRNVFGAREIIALNLGLLPFFLSFVIGRDREAHGGVG
                     LSYRLDLFALACGIACLWIALKRRKPVRDVASRRRRVGLSVGFALLAIIQIARGIGLL
                     HVVIASDGSPVGLAKLGNGPTQPATLHQTALMAAQLS"
     gene            complement(604..1266)
                     /gene="pyrE"
                     /locus_tag="VFU90_11045"
     CDS             complement(604..1266)
                     /gene="pyrE"
                     /locus_tag="VFU90_11045"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5480363.1"
                     /translation="MREFQRQFLDFAIERQVLRFGEFTLKSGRVSPYFMNTGLFNSGK
                     ALALLGRYYAAAIDASGLTFDTLFGPAYKGIPLASAAAIALAEQFGRDVPYSFNRKEL
                     KDHGEGGAIVGAPLQGRILIVDDVITAGTAIRESLAVIESAGARAVGVVVALDRQERG
                     TNELSAIQEIERNYGLSVISIVGLGDVLEYLEGRSGMAHAVDAIRAYRAQYGVVDRAR
                     SG"
     gene            complement(1259..1657)
                     /locus_tag="VFU90_11050"
     CDS             complement(1259..1657)
                     /locus_tag="VFU90_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480364.1"
                     /translation="MLDHLSLGVTDLQRAIRFYDAALAPLGYVRVWTDDAAAGYGGPG
                     GEDRLSIQARADAAPAGPGAHVALTAPSATSVDAFHAAAVCNGGLDDGAPGPRPRYGL
                     GYYAAFVRDPDGNKVEAVHHALLLAGDDHA"
     gene            1814..>2467
                     /locus_tag="VFU90_11055"
     CDS             1814..>2467
                     /locus_tag="VFU90_11055"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU5480365.1"
                     /translation="MADHVNRQFRLAARPVGRVKSTDFTLHEAPDATPGPNQAVARIL
                     YLSLDPTNRIWMSDMDQYMPPVELGEIMRGSAVGQIVASNRADYAVGDLVTGLLGWQD
                     YCLISDGALPLPFKLPPAPVRPSTFLGVLGLTGVTAYFGLHDVGRPQQGDTVVVSAAA
                     GAVGSIAGQLAKLHGCRVVGIAGSDDKCDWLTGELGFDAAINYRHPEWKAQLRAACPD
                     "
BASE COUNT          419 a          860 c          804 g          384 t
ORIGIN      
        1 cgagagctgc gcggccatca gcgccgtctg gtgaagcgtc gctggttgcg tcgggccgtt
       61 gcctagcttg gcgagtccga ccggcgatcc gtcggacgcg atgaccacgt gcagcagtcc
      121 gatcccacgt gcgatctgga tgattgcgag cagcgcgaat ccgaccgaca ggcccactcg
      181 acgtcgtcga gacgcgacgt cgcgcaccgg cttccgtcgt ttcagcgcga tccagaggca
      241 ggcgatgccg catgcgagcg cgaacagatc gagccggtag ctcaatccca ccccaccatg
      301 cgcctcgcga tcgcgaccga tgacgaagga aaggaaaaac ggcagcaggc cgaggttgag
      361 cgcaatgatc tcgcgagcgc cgaacacgtt ccgattggag gaggcaggaa cgtccaccga
      421 cccacgctag catctgccgg cgcaggcacg cgtgatccgt cccacgccgc tgcagtttct
      481 ctcggcgggg agattcgcca tcgcacctac gccgtccgcg tcttcccaaa gagggacagc
      541 gtagacgcgg gctcacacgg atcgaaggga ccgtcagatc catcgtcctg cgccgcggga
      601 tcgtcagccc gaacgggccc ggtcgacgac tccatactgc gcgcgatacg cacggatcgc
      661 gtcgaccgcg tgagccattc ccgaacgacc ctccagatat tcgagcacgt ctccgagccc
      721 gacgatgctg atgacgctga ggccgtagtt acgctcgatc tcctgaatgg cggacaactc
      781 gttcgtgcca cgctcctgtc ggtcgagcgc gaccacgacg ccaaccgccc gggcgcccgc
      841 gctctcgatc acggcaagcg actcgcggat ggccgtgccg gccgtgatca catcgtcgac
      901 gatcaagatc cgcccctgta gcggcgcacc gacgatggct cccccttctc cgtggtcctt
      961 gagctccttg cgattgaagg aatacgggac gtcgcgacca aactgctcgg cgagggcgat
     1021 cgctgcggcg ctggccaggg gaatgccctt gtacgcaggg ccgaagagcg tgtcgaacgt
     1081 gagaccggag gcgtcgatcg ccgccgcgta ataccgaccg aggagcgcca atgccttacc
     1141 gctattgaac agcccggtgt tcatgaagta ggggctgacc cgccctgatt tgagcgtgaa
     1201 ttccccaaat ctcagcacct gccgttcgat cgcaaagtcg agaaactgac gctggaactc
     1261 acgcatggtc gtcacctgca agcaagaggg cgtggtgcac ggcttctacc ttgttgccgt
     1321 cgggatcgcg cacgaacgcc gcatagtatc cgagcccgta cctcgggcgt ggtcccggcg
     1381 cgccgtcgtc gaggcctccg ttgcacacgg ccgccgcatg gaacgcgtcg accgacgtgg
     1441 cgctcggcgc cgtcagcgcg acgtgcgcgc ccggacccgc cggcgcggcg tccgcacgcg
     1501 cctggatcga caagcgatct tccccgcccg gcccgccgta gccggcggcg gcgtcgtcgg
     1561 tccagacgcg gacgtacccg agcggtgcga gcgccgcgtc gtagaagcga atggcgcgtt
     1621 gcaggtcggt gacgccgagc gacaggtggt cgagcacgag ccgagattac accgccgccc
     1681 ggggacccga ccgtcagccc gcgacgattt tgcttgtggc gttattaaga attttcttaa
     1741 ctgcggtcgc gcaggagccg cgtatgccgc gcggcgcccg ctgcgctcat tcgaggcgtc
     1801 gtaggagggg gccatggccg atcacgtgaa ccgtcaattt cgtctcgcag cgcgcccggt
     1861 ggggcgcgta aagtcgaccg acttcaccct gcacgaagcg ccggacgcca cgccgggtcc
     1921 caaccaggcg gtggcgcgaa tcctctatct ctcgctcgat cccaccaatc gcatctggat
     1981 gagcgacatg gaccagtaca tgccgccggt ggagctgggt gagatcatgc gcggctccgc
     2041 ggtgggccag atcgtcgcct ccaaccgcgc cgactacgcc gtgggcgacc tggtcaccgg
     2101 cctcctcgga tggcaggact actgcctcat ctccgacggc gcgctgccgt tgccgttcaa
     2161 gctgccgcca gcgccggtgc ggccgtcgac ctttctcggc gtgctcgggc tgaccggcgt
     2221 caccgcctat ttcggcctcc acgacgtcgg ccgaccgcag cagggcgaca ccgtcgtggt
     2281 ctcggcggcg gcgggcgccg tgggcagcat cgccggccag ctggccaagc tccacggctg
     2341 ccgcgtggtc ggcatcgccg gcagcgacga caagtgcgac tggctcaccg gcgagctcgg
     2401 cttcgacgcc gccatcaact accggcaccc cgagtggaag gcgcagctgc gcgccgcctg
     2461 ccccgac
//
LOCUS       DASUCJ010000926         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_957298, whole genome shotgun sequence.
ACCESSION   DASUCJ010000926 DASUCJ010000000
VERSION     DASUCJ010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957298"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1522)
                     /locus_tag="VFU90_11060"
     CDS             complement(8..1522)
                     /locus_tag="VFU90_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480366.1"
                     /translation="MDKNLFRVLLTGAAFALLGGGTAFAQATPAPAATAKPSAFTYSG
                     FLRAYYFTRTNFPATSKTPNQASFDPAISLHAGYDFGGGFSVGGTYLYANPFNNCDDP
                     ASHLHQPCAKHPVTNPPESNPDDTLPGYRMSTLYEAYLQYKDANALARLGDQVLNNPW
                     ANPSDSRLKPAAFRAADVAYKFDPNWTIEGVYANRFEDRVQSDFVDGTLLTQNGSFPD
                     APGVGNTGIPAGGVVHNNGFYYGRIGYTGGSLAANLHYYAFDQIANALWFDGKYSWKA
                     YGKPFIAVQAGSEANTGTSFIGKISSSLFGVQAGYSPWSTVDLTVGADFIPVKSDTIT
                     LPLTASCGANDEIKGTLMYFLPSGGTPNCKTSSPGVPGAVATVYYGGWASPYTDSYAT
                     DPLFDTSISQGMVDRRSPGSGVKVAGTFYTDQKQVRLIVSHAWYVYGNDAVGISPTQE
                     TDVDGTYFFSRVGKGAYHGFSLRHRYAERTQTFTAAFGGLPDFKYNRTQLEYDF"
BASE COUNT          279 a          446 c          514 g          313 t
ORIGIN      
        1 ccggtcgtta gaagtcgtat tccaactggg tgcggttgta tttgaagtcg ggcaatccgc
       61 cgaacgccgc ggtgaacgtt tgcgtgcgtt cggcgtagcg atgacgaagc gagaatccgt
      121 gatacgcgcc tttgccgacc ctactgaaga aataggtgcc gtcgacgtcc gtctcttgcg
      181 tcggcgaaat gccgacggca tcgttgccgt acacgtacca cgcgtggctg acgatcaggc
      241 gaacctgttt ctgatcggtg tagaacgtgc cggcgacttt cacgccggaa cccgggctgc
      301 ggcgatccac catgccctgg ctgatgctcg tatcgaagag cggatcggtc gcgtacgaat
      361 cggtatacgg gctggcccag ccgccgtaat acacggtcgc gaccgcaccg ggtacgccgg
      421 ggctcgacgt cttacagttc ggcgttccgc cggacggcaa gaagtacatc agcgtgcctt
      481 tgatctcgtc gttcgcgccg cacgatgcgg taagcggcag cgtgatcgtg tctgacttca
      541 ccggaatgaa atccgcgccg acggtgaggt cgacggtgct ccacggcgag tatccggcct
      601 gcacgccgaa gagcgagctg ctgatcttgc cgatgaacga cgtgccggtg ttggcttcgc
      661 tgccggcctg cacggcgatg aacggcttgc cgtaggcttt ccatgagtac ttgccgtcga
      721 accagagcgc gtttgcgatt tggtcgaacg catagtagtg cagattcgcg gcaagcgaac
      781 cgcccgtata gccgatgcgg ccgtagtaga agccgttgtt gtgcaccacg ccgccggcgg
      841 ggatgccggt attgccgacg cccggcgcat ccgggaacga gccgttctgc gtgagcagcg
      901 tgccgtcgac gaaatccgac tgcacgcgat cctcgaagcg gttcgcgtac acgccctcaa
      961 tcgtccagtt cggatcgaac ttgtacgcga cgtcggcggc gcggaacgcg gccggcttga
     1021 gccgcgaatc cgacggattc gcccatgggt tgttgagcac ttgatcgccc aagcgcgcga
     1081 gcgcgttggc atctttgtac tgcagatacg cttcgtacag cgtgctcatg cgatagccgg
     1141 ggagcgtgtc gtcaggattg ctctccggcg gattggttac cgggtgcttc gcgcacggct
     1201 gatgcagatg cgaagccgga tcgtcgcagt tgttgaatgg attggcgtag agatacgttc
     1261 cgccgacgga gaagccgccg ccgaagtcat agcccgcgtg caagctgatt gcgggatcga
     1321 agctggcttg attcggcgtc ttcgaggtcg ccgggaaatt ggttctggtg aaatagtacg
     1381 cgcggagaaa tccgctatac gtgaaggcgc tgggcttggc cgtcgccgca ggcgccggcg
     1441 ttgcctgggc gaacgcggtt ccgcccccga gcagcgcgaa agccgcgccg gtcaaaagga
     1501 cgcggaagag gtttttgtcc atggcgatta ttgtatcctc ggattcttaa ct
//
LOCUS       DASUCJ010000927         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_346194, whole genome shotgun sequence.
ACCESSION   DASUCJ010000927 DASUCJ010000000
VERSION     DASUCJ010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346194"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="rph"
                     /locus_tag="VFU90_11065"
     CDS             <1..624
                     /gene="rph"
                     /locus_tag="VFU90_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5480367.1"
                     /translation="ISFGTTRVLCTASVEDGVPGWRRGSGEGWLTAEYAMLPRATRTR
                     TARERAQLGGRTQEIQRLIGRSVRAMLDDFKFGEFTVKLDCDVLVADGGTRTAAITGA
                     AVAIVDAFEWMVGNGKLVQSPVKRRVAAVSVGMIDGEPRLDLDYEEDVRAEVDMNVVM
                     SNEGKFVEVQGTGEHGTFDRRQLNELLDLAIAGIRALDAAQAKALAE"
     gene            621..>1084
                     /locus_tag="VFU90_11070"
     CDS             621..>1084
                     /locus_tag="VFU90_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEU5480368.1"
                     /translation="MKRGRYLLATRSEGKLRELRQIFADFQLEVINLRDAGIERHANE
                     DDLEQYATFEENALAKVRYFFDRSNGLPTFADDSGLCVDALNGEPGVFSKRWSGRGDL
                     PEPAIDVANNEKLIESMQKARAEQGPRFTDAAKYVAVAAYKDATMEVVRRGE"
BASE COUNT          212 a          301 c          380 g          191 t
ORIGIN      
        1 atctctttcg gtacgacccg agtgctttgc acggcgtcgg tcgaggatgg agttcccggc
       61 tggcgacgtg gcagcggtga aggctggctc accgcggaat acgcgatgct gccgcgcgcg
      121 acgagaactc gcaccgcgcg cgagcgtgcg cagctcggcg ggcgcaccca ggaaattcag
      181 cgactcatcg gtcgcagtgt ccgggccatg ctcgacgatt tcaagttcgg cgagttcacc
      241 gtgaagctcg attgcgatgt gctcgtcgcc gacggcggta ctcgtaccgc tgcgatcacg
      301 ggcgccgcgg tggcgatcgt cgatgcgttc gagtggatgg ttgggaatgg caaactggtg
      361 caaagtccag taaagcggcg ggtagcagcg gtgagcgtcg gtatgatcga tggtgagccg
      421 aggctcgatc tggactacga ggaagacgtt cgcgccgaag tcgacatgaa tgtcgtgatg
      481 agcaacgaag gcaagttcgt ggaggtgcag gggaccggcg agcacggcac tttcgaccgc
      541 cgccagctga atgagctgct cgatttggcg attgcgggga tccgggcgtt ggacgcggct
      601 caggcgaaag cgctcgcgga gtgaaacgcg gtcgttatct gctcgcgacg cggagcgaag
      661 gtaagctgcg cgagctgcgc caaattttcg ccgattttca gctcgaagtg atcaatctgc
      721 gcgacgccgg aatcgagcgc cacgccaacg aggatgactt ggagcagtac gccacttttg
      781 aagagaacgc gctcgccaag gtgcgctatt tcttcgaccg ctccaacggg ctgccgacct
      841 ttgccgacga ttccggtctt tgcgtcgacg cactgaacgg cgaacccggt gtctttagca
      901 aacgatggtc gggccgaggc gatttgccgg agcctgcgat cgacgtcgcc aacaacgaga
      961 aactgatcga atcgatgcag aaggcgcggg cagagcaggg cccgcggttc acggacgccg
     1021 cgaagtacgt cgccgtcgcg gcgtacaagg acgcgaccat ggaagtcgtg cgccggggcg
     1081 agat
//
LOCUS       DASUCJ010000928         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_177861, whole genome shotgun sequence.
ACCESSION   DASUCJ010000928 DASUCJ010000000
VERSION     DASUCJ010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177861"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFU90_11075"
     CDS             complement(<1..540)
                     /locus_tag="VFU90_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480369.1"
                     /translation="MKIRNGLAALALLAACFGTAPATAASAVTLYDGTVINARLNQGL
                     SSGSAHVGDAFSMTVVPPYPSGDPSFQGATVTGEVTHVQPAGQGRNPEIVIVPRYLHL
                     ADGSTAYLHGNVTSVAANKSTGETAGKAAIGALAGMLIGNAIGKTIFHASGGGAVGLI
                     GGAMLGANSKSNFSIPAGAN"
     gene            complement(602..1201)
                     /locus_tag="VFU90_11080"
     CDS             complement(602..1201)
                     /locus_tag="VFU90_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480370.1"
                     /translation="MKRKRAILAAAAAAALTLAAAGSALAATIASGTQIRCVLAQGVN
                     SANLTVGQDFKLVIDDPAQPSLEGAAIHGHVTDVAGPGGMSRARIGFMIDFIRFKNGT
                     RESIHANVVSRNVVQTNTAVARQESVKFALPPMPTGTVTPGPIAWQMHFRRDAAPSVT
                     PPPAGNTSGYVYAQKSNENIVIPPGSPVTIQLTSSLTTP"
     gene            complement(1235..>1785)
                     /locus_tag="VFU90_11085"
     CDS             complement(1235..>1785)
                     /locus_tag="VFU90_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEU5480371.1"
                     /translation="LSDANTRIFVNPTGRFVIGGPKGDAGLTGRKIIADTYGGMARHG
                     GGAFSGKDPTKVDRSAAYAARWVAKNVVAAGLADRCEVQVAYAIGVAHPMNVFVETFG
                     TAKIDEELIAKAVTEVFDLRPAAIIHHLDLRRPIYRQTAAYGHFGRPDLDLPWERLDR
                     VDALRDATGFTGEALRVPNFAQ"
BASE COUNT          267 a          591 c          588 g          339 t
ORIGIN      
        1 gttggctccg gccgggatgc tgaagttgct cttgctgttg gcgcccagca tcgcgccgcc
       61 gatcagcccg accgctccgc cgccgctggc gtggaagatc gtcttgccga tagcgttgcc
      121 gatcagcatt ccggccagcg cgccgatcgc cgccttgccg gccgtttcgc cggtgctttt
      181 gttggccgcg acgctggtca cgttgccgtg cagataggcg gtgctgccgt cggccagatg
      241 caggtatcgc ggcacgatca cgatttcggg attgcgtcct tgccccgcgg gctgcacgtg
      301 cgtcacttca ccggtgacgg tggcgccttg gaagctcgga tcgccgctgg gatacggcgg
      361 aacgaccgtc atcgagaatg cgtcgccgac gtgcgcgctg ccgctgctca gcccttgatt
      421 gagacgcgcg ttgatgacgg tcccgtcgta gagcgtcacc gcacttgcgg ccgtcgcggg
      481 cgccgtgccg aagcacgccg caagcagtgc gagcgcggcc aggccgttgc gaattttcat
      541 gtctacccta tttcccccat tgcaggcttg ctcggatggc agcctgactc gcggagaagt
      601 tttagggcgt cgtcagactg ctcgttagtt gaatcgtaac cggcgagccc ggcggaataa
      661 cgatgttttc attagatttc tgtgcgtaaa cgtagccgct cgtgttgccg gccggcggcg
      721 gcgtaaccga cggcgccgcg tcgcggcgaa aatgcatctg ccaagcgatc gggcccggcg
      781 tcaccgttcc ggtcggcatc ggcggcagcg cgaacttcac cgactcttgc ctggcgaccg
      841 ccgtgttggt ctgcacgacg ttccggctga cgacgttggc gtgaatcgac tcgcgcgttc
      901 cgttcttgaa gcggatgaag tcgatcatga agccgattcg agcgcgcgac atcccgcccg
      961 gaccggcgac gtcggtaacg tgtccgtgaa tcgccgcacc ttccaacgac ggttgcgccg
     1021 gatcgtcgat caccagcttg aaatcctggc cgaccgtgag gtttgcggag ttgacgcctt
     1081 gggccagcac gcagcggatt tgggttccgc tcgcgatggt ggcggcgagc gcgctgcccg
     1141 cggccgcgag cgtcagcgcg gccgccgccg ctgctgcaag aatcgcgcgt ttgcgtttca
     1201 accgagtgct cctctccgcc gcgtcagcgg cgcgctattg ggcgaagttc gggacgcgca
     1261 gcgcttcgcc ggtaaaaccg gtcgcgtcgc gcagcgcgtc gacgcgatcg agccgctccc
     1321 acggcagatc caaatccgga cgcccgaaat gtccgtaggc ggccgtctgc cggtagatcg
     1381 gccggcgcag atccaggtgg tggatgatcg ccgccgggcg cagatcgaac acttcggtca
     1441 ccgccttggc gatcagctct tcgtcgatct tcgcggtgcc gaacgtttcg acgaacacgt
     1501 tcatcggatg ggcgacgccg atcgcgtagg cgacctgcac ttcgcagcga tccgctaacc
     1561 ccgccgccac gacgttcttg gcgacccagc gcgccgcgta agcggccgaa cggtctacct
     1621 tcgtgggatc tttcccggag aacgcgccgc cgccgtgacg cgccatcccg ccgtaggtgt
     1681 cggcgatgat cttccgcccg gtgagcccgg cgtcgccttt cggaccgcca atgacgaagc
     1741 gccccgtcgg gttgacgaaa atccgggtat tcgcatcgct cagcg
//
LOCUS       DASUCJ010000929         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_697422, whole genome shotgun sequence.
ACCESSION   DASUCJ010000929 DASUCJ010000000
VERSION     DASUCJ010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697422"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFU90_11090"
     CDS             <1..528
                     /locus_tag="VFU90_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480372.1"
                     /translation="STISRSSAAFSGGYVHPKPYGTNAAVGTPADSAPRCAAVSMPSA
                     SPAITGRPSRAASATANTAASGGGVRLPTIATLRAPGESADPEAFVERDRLQAEYSVA
                     VVLVASELGGGALHTLEFACELARPRFALQPPGAAEGRPEWAGNLNALAGGAFPLPFD
                     LTEAIRILNDRLDGT"
     gene            506..1801
                     /locus_tag="VFU90_11095"
     CDS             506..1801
                     /locus_tag="VFU90_11095"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU5480373.1"
                     /translation="MTDSMVRSAADRARQVLAGGCDSPVRSGWSVAAPPFVQTDARGA
                     YAYDETGRRYVDYIMAYGPLLFGHTHPALVGGLDAIARGGFVWGNTHPEEIRLAERVR
                     AHLPSMERLRFVTSGTEAVASAIRVARAHTHRSLILKFAGNYHGHSDVALLDAGASAH
                     TSDAARGGIPAGVAADVAIARYNDLASVERALQGREERLAAIVVEPIAGNMGFVAPEA
                     GFLQGLRERANAHGALLIFDEVMTWLRIGLEGAQGRLGIVPDLTTLGKIMGGGAPIAA
                     FGGRADVMAALAPEGTAFTGGTHGGNPFCVGMAHRVLDLLEAHPEYYARMDALAKRLA
                     DGVRRILARRGLPYAVVQYESIVDFKFRPGPPGRNYDDARAADRNAYAAYYRAMLERG
                     ILLPPSQNEVMFVSTAHEEADVEQTLGAVDESLGAAAGA"
     gene            complement(1815..2099)
                     /locus_tag="VFU90_11100"
     CDS             complement(1815..2099)
                     /locus_tag="VFU90_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEU5480374.1"
                     /translation="MGDPIPNQSRKAFLGSVILLPALASLGTAAAKADAAKSSQASMQ
                     YQSTPKDGKHCANCNFFIPGESATANGTCKIVDGTISPTGWCIAYNASGS"
     gene            complement(2226..>2963)
                     /locus_tag="VFU90_11105"
     CDS             complement(2226..>2963)
                     /locus_tag="VFU90_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480375.1"
                     /translation="ARPAPVLAVLNNDIVGNSTGGDGQREPDVIRVFSQGLPAGASLD
                     NVNLIGSENDSPSRELSRFVADVVPAYVPGFSVRQIFRADRFLRGGDQESFQDAGYAA
                     AIRFVEPHENFTHQHQDVRVQDGVQYGDLPQYVDPEYLARATQANIAALATLALGPAP
                     PASVELVTKRLQYDSTLRWKPSSGAVSYEILWRATDAPQWQFARNVGNVTEATVPVSK
                     DDDVFGVRSVGDAGLRSPAVYPKPVRE"
BASE COUNT          460 a          956 c         1054 g          493 t
ORIGIN      
        1 tcgacgattt cgcgttcgag cgcggcgttc tcgggcggat acgtgcaccc gaaaccgtac
       61 ggaacgaacg ccgcggtcgg cactccagcc gatagcgcgc cgcgatgcgc ggccgtgtcg
      121 atgcccagcg ccagccccgc gatcaccggg cggccgagtc gcgccgccag cgcgaccgcg
      181 aacaccgccg cctcgggcgg cggcgtccgg cttccgacga tcgcgacgct tcgcgcgccc
      241 ggcgagagcg ccgatccgga ggccttcgtc gagcgggacc gcctgcaggc cgaatactcg
      301 gtcgcggtcg tgctggtggc gagcgagctc ggcggcggcg cgctgcatac gctggagttt
      361 gcgtgcgagc tggcgcggcc gcggtttgcg ctgcagccgc ccggggcggc ggaggggcgt
      421 cccgagtggg ccggaaattt gaacgcgcta gccggcggcg cgttcccgct tcccttcgat
      481 ctcaccgagg ctatacgaat tctcaatgac cgactcgatg gtacgtagcg ccgcggaccg
      541 cgcgcggcag gtgctggccg gcggatgcga ttcaccggtt cgctcgggat ggtcggtggc
      601 ggcgccgcca ttcgtgcaga cggacgctcg cggcgcgtac gcgtacgacg aaaccggccg
      661 ccggtacgtc gactacatca tggcctacgg gccgctgctg ttcggccaca cgcatccggc
      721 gctggtcggc ggtttggacg cgatcgcacg cggcggtttc gtttggggga acacgcatcc
      781 ggaagaaatc cggctggcgg agcgcgtgcg cgcacatctt ccgtcgatgg agcggctgcg
      841 tttcgttacg agcggtaccg aggcggtggc gagcgcgatc cgcgtcgcgc gggcgcacac
      901 ccaccgcagc ctcatcctaa agtttgccgg caactatcac ggccactccg acgtggccct
      961 gctcgacgcc ggggcctcgg cgcacacgtc cgacgccgcg cgcggcggca ttccggcggg
     1021 tgtcgccgcc gacgtcgcga tcgcgcgcta caacgatctg gcatcggtcg aacgcgcgct
     1081 gcagggtcgc gaagagcggc tggcggcgat cgtggtggaa ccgatcgccg gcaacatggg
     1141 attcgtcgct ccggaagccg gctttcttca ggggctgcgc gagcgcgcga acgcgcacgg
     1201 cgcgctgctg atcttcgacg aggtgatgac gtggctgcgg attgggctcg agggcgcaca
     1261 agggcgcctc ggaatcgttc ccgatctcac cacgctcgga aagatcatgg gcggcggcgc
     1321 accgattgcg gccttcggcg ggcgtgccga cgtcatggcg gcgctggctc cggaggggac
     1381 cgcttttacc ggcgggacgc acggcgggaa tccgttctgc gttggaatgg cgcatcgcgt
     1441 tctcgatctg ctcgaggccc atccggaata ctacgcacgg atggacgcgc tggcaaaacg
     1501 cttggccgac ggagttcggc ggatcctcgc gcgacgcggt ttaccgtacg cggtcgtgca
     1561 atacgaatcg atcgtcgact tcaagtttcg cccgggtccg cccggccgca actacgacga
     1621 cgcgcgggcg gccgaccgca acgcctatgc ggcctactat cgcgcgatgc tcgaacgcgg
     1681 catcttgctg ccgccgtcgc agaacgaggt gatgttcgtt tcgacggccc acgaagaagc
     1741 cgacgtcgag caaacgctcg gcgcggtcga cgagagtctg ggcgccgcgg ccggcgctta
     1801 gacgaacggg tcgcttacga cccgctggcg ttgtacgcga tgcaccagcc ggtcggcgaa
     1861 atcgtgccgt cgacgatctt gcaggtgccg ttcgcggttg cgctctcccc cggaatgaag
     1921 aagttacagt tggcgcagtg cttcccgtct ttgggtgtgc tctgatactg catcgacgct
     1981 tgcgagctct tggccgcgtc cgctttggcg gcagcggtac cgagtgaggc aagggccggc
     2041 agcaggatga cgctccccag gaacgccttc cgggactgat tggggatagg gtcgcccatg
     2101 ggtgtacggc ctccgtccat tcgtagtgcc tcgactctac gaccccaccg tgaaaaaaac
     2161 ctgaaaattg gtcgccgccg agccggctac ggcgccggct cgacgagcgg ggcgggcatc
     2221 ggcggctact cgcggaccgg tttcgggtac accgccgggc tgcgcagacc ggcgtcgccg
     2281 acgctgcgta cgccgaagac gtcgtcatct ttgctgaccg gaacggtcgc ttcggttacg
     2341 ttgccgacgt tgcgcgcgaa ctgccactgc ggggcgtcgg tcgcgcgcca gaggatctcg
     2401 taggacaccg cgccggacga cggtttccag cgcagcgtcg aatcgtactg caggcgtttg
     2461 gtgaccagtt cgacgctggc cggcggggcg gggccgagcg ccagcgtggc gagtgcggcg
     2521 atattggctt gcgtcgcgcg cgctaaatat tcggggtcga cgtactgcgg caagtcgccg
     2581 tattgcacgc cgtcctgcac gcgtacatcc tgatgctggt gcgtgaagtt ctcgtgcggc
     2641 tcgacgaagc gaattgcagc cgcatagcct gcgtcttgga acgactcctg gtcgccgccg
     2701 cgcagaaacc gatcggcgcg aaagatttgc cgtacggaaa aaccgggaac gtatgccggc
     2761 acgacatcgg caacgaagcg cgaaagctcc cgcgagggcg agtcgttttc ggagccgatc
     2821 agattcacgt tgtctaacga agcgccggcc ggcaagcctt ggctgaaaac gcggatcacg
     2881 tcgggctcgc gttgtccgtc gccgccggtg ctgttgccga cgatgtcgtt attgagaacg
     2941 gcgagaacgg gggcggggcg cgc
//
LOCUS       DASUCJ010000930         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_560216, whole genome shotgun sequence.
ACCESSION   DASUCJ010000930 DASUCJ010000000
VERSION     DASUCJ010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560216"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFU90_11110"
     CDS             <1..564
                     /locus_tag="VFU90_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5480376.1"
                     /translation="LDTSRKGYLSAVSVSAYSMVALVQRLGPLVRPGGSFLSLSYLAA
                     ERVIPGYGGGMSSAKAALESDTRVLAWEAGRKWGHRVNCISAGPLASRAASAIGRGSD
                     REEDRQKETAAGGADGTKRKSFIEQMVDYVRANAPLPQALEAEDVGSVAAFLSSPLAQ
                     AVTGCVVHVDKGYHAMGKSVATAESSA"
     gene            628..>1171
                     /locus_tag="VFU90_11115"
     CDS             628..>1171
                     /locus_tag="VFU90_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5480377.1"
                     /translation="MTAPTNAFIPPAHGEPITMGPNHRLVVPNNPIVPFIEGDGTGRD
                     IWRASVRVIDAAVEKAYGGKKKIAWYEVYAGEKAFNTFNNWLPDETVAAFRKYLVGIK
                     GPLTTPIGGGIRSLNVALRQLLDLYACVRPVRYYKGTPSPVKNPERMDIVIYRENTED
                     VYAGIEWEQGTAECARLIEFI"
BASE COUNT          219 a          392 c          381 g          179 t
ORIGIN      
        1 ctcgacacga gccgtaaggg ttatctctcg gccgtcagcg tcagcgcgta ctcgatggtc
       61 gccctcgtgc agcgactcgg cccgctcgtg cgtcccggcg ggtcgtttct cagtctgtcg
      121 tacctcgccg cggagcgggt aatcccgggc tatggcggag ggatgtcgag cgccaaagcg
      181 gccctcgaga gcgacacgcg cgtgctggcg tgggaagcgg ggcgaaaatg gggccaccgc
      241 gtcaactgca tcagcgccgg cccgctcgcg tcccgcgccg cgagcgccat cggtcgcggg
      301 agcgaccgcg aagaagaccg tcaaaaggag acggcggcgg gcggcgcgga cggcacgaag
      361 cgcaagtcgt tcatcgagca gatggtcgac tacgtgcgcg ccaacgcccc cttaccgcag
      421 gccctcgaag ccgaggacgt cggttcggtc gcggcgttcc tttccagccc cctcgcgcag
      481 gccgtcaccg gctgtgtcgt ccacgtggac aaggggtatc acgcaatggg caaaagcgta
      541 gcgaccgccg agtcttcggc gtagtctgcc gccgctgctc gcgtttgccc gaagcaacgt
      601 tcatcgcaaa gaaagacacc gccgcccatg accgcgccca cgaatgcctt catccccccg
      661 gcccacggcg aacccatcac gatggggccg aaccaccggc tcgtcgttcc gaacaacccg
      721 atcgtgccct tcatcgaggg tgacggcacc ggccgcgaca tctggcgcgc gtcggttcgc
      781 gtgatcgacg cggcggtgga aaaggcctac ggcggcaaga agaagatcgc ctggtacgag
      841 gtgtacgcgg gcgagaaggc gttcaacacg ttcaacaact ggcttccgga cgagacggtc
      901 gcggcgttcc gcaagtatct cgtcggaatc aaaggcccgc tcaccacgcc gatcggcggc
      961 ggcatccggt cgctcaacgt cgcgctgcgg caacttctcg acctctacgc ctgcgtccgt
     1021 ccggtgcgct actacaaagg aacgccctcg ccggtgaaga atcccgaacg catggacatc
     1081 gtcatttacc gcgagaacac cgaagatgtt tatgccggga tcgaatggga gcagggaaca
     1141 gcggagtgcg cccggctgat cgagttcatc a
//
LOCUS       DASUCJ010000931         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_926987, whole genome shotgun sequence.
ACCESSION   DASUCJ010000931 DASUCJ010000000
VERSION     DASUCJ010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926987"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFU90_11120"
     CDS             <1..536
                     /locus_tag="VFU90_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480378.1"
                     /translation="MNRFAASLSIKRTLGVMIMLPAILAAGALVASACAGTKAAAQNK
                     PAPSYAAQTREFTISIVPLVTHEQQSYADYLQKDFAPGGVLDGKEIYGYNPSTFTVYQ
                     GDTVNITWVNPTDDTHINVIPDFAVNVSQDGESTAHSTFVANKVGTFTFLCAEAEHSP
                     YMAGQIVVLPDSDGPQQ"
     gene            complement(621..>1028)
                     /locus_tag="VFU90_11125"
     CDS             complement(621..>1028)
                     /locus_tag="VFU90_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5480379.1"
                     /translation="LGAEPVDGANAAPEIAAKTNMRGVDCAVEASGSDATVGMAIAVT
                     GLGGVASVVGVNLTPAFQFPMALAFVKNMTFRIGTCSVQCHWPELVPLVRAGRLHPER
                     VVSHHLPLSQGADAYRLFDAREDGALKMVLTAS"
BASE COUNT          190 a          356 c          331 g          151 t
ORIGIN      
        1 tgatgaaccg gttcgcagca agtctctcga tcaagcggac gctaggagtg atgatcatgc
       61 ttccggcgat cctggcagcc ggcgcgctcg tggcgagcgc ctgcgccggc acgaaggcgg
      121 ccgcgcagaa caagccggcg ccgagctatg cggcgcagac gcgcgagttc acgatcagca
      181 tcgtgccgct ggtcacacac gagcagcagt cgtacgccga ctacctgcag aaggacttcg
      241 cgccgggcgg cgtcctcgac gggaaggaga tctacggtta caacccgagc acgttcaccg
      301 tctaccaggg cgacacggtg aacattacct gggtcaaccc gacggacgac acgcacatca
      361 acgtgatccc cgacttcgct gtaaacgtca gtcaggatgg ggaatcgacc gcacattcga
      421 ccttcgtcgc gaataaggtg gggacattca ccttcctctg cgcggaggcc gagcactccc
      481 cgtacatggc gggacagatc gtggtcctgc cggacagcga cggcccgcag cagtagcgtt
      541 ccggacacac gcggtgcaca cgggcgaggc gagggtagcc tcgcccgtcc gcatctccgg
      601 gacgtgctcc gcgtctgctc tcaggacgcg gtgagcacca tcttcagcgc gccgtcttcg
      661 cgcgcgtcga acaggcgata cgcatcggca ccctgcgaca gcggcaggtg atgcgacacc
      721 acgcgctccg ggtgcagccg gcccgcccgc accagcggca ccagctccgg ccagtggcac
      781 tgcacggagc acgtgccgat gcggaacgtc atgttcttga cgaaagcgag cgccatcggg
      841 aactggaacg ccggcgtcag attcacgccg acgactgatg cgacgccgcc gagccccgtg
      901 actgcgatcg ccatgccgac cgtcgcgtcc gaaccggatg cctcgaccgc gcagtcaact
      961 ccgcgcatgt tcgtcttcgc ggcgatctcc ggggccgcgt tcgcgccgtc gaccggctcg
     1021 gcgccgag
//
LOCUS       DASUCJ010000932         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1492481, whole genome shotgun sequence.
ACCESSION   DASUCJ010000932 DASUCJ010000000
VERSION     DASUCJ010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492481"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFU90_11130"
     CDS             complement(<1..890)
                     /locus_tag="VFU90_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480380.1"
                     /translation="MNDYRRSLHALAAAALALGAAACQGTPSQGSTYVPNAGAEAVHR
                     AARSTDLLYASTLDWNAGTGSVYVYRAYGKNTASIGSIGFQTGFPDGLWTDASGRVYV
                     AVVNAGTNGHGYVNVYAPGLKKLLKTYAAGLDGPSGGTFDGAGNMYVSNLCGTLPSIG
                     CYVFARTRNGKHLEPQSSSAFSGYVAVFPKGSRQPTQDLQNGINIAVGVTIDRAGNVF
                     VPDNTGQAAWDVIEFPAGSSQGHVVPFQNLPSQLWIGAATFDPKGALVISANDAIAFF
                     PHERGKPSHTLTDGVVAPDG"
     gene            1051..>1827
                     /locus_tag="VFU90_11135"
     CDS             1051..>1827
                     /locus_tag="VFU90_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480381.1"
                     /translation="MSFRFVRYAAAVAALVLAACSGGPSVTTPPSGTAAMPSITVVNG
                     EHVVRVGAQVFRFGGHPVHRAARRSWLSPAAGSNPLLFGSSYNGGFIQVYSQKGTNQS
                     PIGQLSNGLLSPQGMAVDQNRRLWVANTNAFTVVGFRHGATSPFVTLKDPNYYPIQVA
                     VDGNGTVYASNAESTTGPPGNVTFWRKGAKKPSGTLTYGNFLIVLGVGVDASNNVYVS
                     YIPTSGPPAMVVFPAGSQTGQPLAIQNANEGQMTFDSAGNL"
BASE COUNT          298 a          630 c          570 g          329 t
ORIGIN      
        1 aaaccgtcgg gcgcgaccac gccgtcggtg agcgtgtgcg acggctttcc ccgttcgtgc
       61 gggaagaacg cgatcgcgtc gttggccgag atcacgagcg cgcctttcgg gtcgaaggtc
      121 gccgcaccga tccacagctg gctcggcagg ttttggaacg gcacgacgtg gccttgcgac
      181 gagccggccg gaaactcgat cacgtcccat gccgcttgac cggtgttgtc cggcacgaac
      241 acgttgccgg cgcgatcgat cgtgacgccg accgcgatgt tgatcccgtt ttgtaagtct
      301 tgcgtgggct gccggctgcc cttcggaaac acggcgacgt aaccggagaa cgccgaactc
      361 gattgcggct cgaggtgctt gccgttgcgc gtccgcgcga acacgtaaca gccgatcgac
      421 ggcagcgtgc cgcacaagtt cgacacgtac atgttgcccg cgccgtcgaa cgtcccgccc
      481 gacgggccgt ccagcccggc ggcgtacgtt ttcagcagct tcttcaatcc cggcgcgtag
      541 acgttgacgt aaccgtgtcc gttggtgccg gcgttgacga ccgcgacgta cacgcgcccg
      601 ctcgcgtccg tccacaatcc gtcgggaaat ccggtttgaa acccgatcga gccgatcgat
      661 gcggtgttct ttccgtacgc gcgatacacg tacacggatc cggtgccggc gttccagtcg
      721 agcgtcgacg cgtacagcag atctgtcgag cgcgccgcgc ggtgcaccgc ttcggcgccg
      781 gcgttaggca cgtatgtgga accctgggac ggcgtacctt ggcaggcggc agcgcccagc
      841 gcgagggcgg cggcggcgag cgcgtggagg gaacggcgat aatcgttcat gcgcgcacgc
      901 ttcgcagcgc gccgcgcgcc ggcccttcgc tcgcccgtgt acctttctgc caaaatcccg
      961 tgcgctggtg cgtccgtccc aggtgcgaat ccgcacaccg ataagcaacc ggcgacttct
     1021 tcatcgcgtc ttgccggagg atccccccgc atgtcgtttc gtttcgttcg ttacgcggcc
     1081 gccgtcgcgg ccctcgtcct cgccgcctgt tcgggcggcc cgtccgtcac caccccgccg
     1141 tcgggcaccg ctgcgatgcc gagcatcacc gtcgtcaacg gcgagcacgt cgttcgcgtc
     1201 ggcgcgcagg tctttcgctt cggcggtcac ccggtgcatc gcgccgctcg ccgcagctgg
     1261 ctgtcgcccg cggccggatc gaatccgctg cttttcggct cgtcctacaa cggcggcttc
     1321 atccaagtct actcgcagaa gggcaccaat cagtcaccga tcggccaact cagcaacggc
     1381 ctgttgagcc cgcaaggcat ggcggtcgat cagaaccgcc gtctgtgggt tgccaacacc
     1441 aacgccttta cggtcgtcgg tttccgtcac ggcgccacgt cgccgttcgt cacgctcaaa
     1501 gatccgaact actatccgat ccaagtcgcc gtcgacggca acggcaccgt gtatgcgtct
     1561 aatgccgaaa gcacgaccgg accgccgggc aacgtgacct tttggcgcaa aggcgcgaag
     1621 aagccgagcg gtacgctcac gtacggaaac ttcctaatcg tgctcggcgt cggcgtcgac
     1681 gcgagcaaca acgtctacgt ctcgtacatt ccgaccagcg ggccgcccgc gatggtcgtg
     1741 ttcccggccg gttcgcagac cggacagccg ctggcgattc aaaacgccaa cgaaggtcag
     1801 atgacgttcg attcggccgg taacctc
//
LOCUS       DASUCJ010000933         1484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_330526, whole genome shotgun sequence.
ACCESSION   DASUCJ010000933 DASUCJ010000000
VERSION     DASUCJ010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330526"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFU90_11140"
     CDS             complement(<1..591)
                     /locus_tag="VFU90_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480382.1"
                     /translation="MTIMLGRALMSRTPFLLGVVMCLTRSATSGAQGILLQIHPHVGD
                     TLHLRLDQQTELTGTRRANGTPSLATITTSLHVFSRAIIERSAPAGTYVRAITDSVRL
                     TGSDEALSDEARRAVEGRTMLLRIAPDGTVALVDANGDDLSAGADAVSLMPAAFPKGP
                     ISVGYTWSRDMPLPAGGRLAPGSAPAAGWLHTKFRLD"
     gene            complement(588..>1484)
                     /locus_tag="VFU90_11145"
     CDS             complement(588..>1484)
                     /locus_tag="VFU90_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480383.1"
                     /translation="WRTHHIATIRKVRNRLSNISTRCAGLVTDDGNVGDLPFGSFQDR
                     TVYVIDVASLEEDAQDLVFARVVTKLREHLEKRDLGVNHVVVFVDELNKYAPSDGQDT
                     YVRKMLLDLAERGRYLGLVLFSAQQFRSQVHRRVVGNSGTALYGRMDADELATPGYAV
                     LSPAVKTKLATLEKGQLMVRHPHFTQPIFVRFPRPAVMRGRDGAERFPQAQDVTIEAA
                     VLRSLRQLDPTLTLNWVQDAIGIREPDEVIRARNATIRERPADVKRFFKSQLAPIVPA
                     TPAPRPMVTPIRSAPSDDPYGF"
BASE COUNT          249 a          494 c          489 g          252 t
ORIGIN      
        1 atccaatcgg aacttcgtgt gcagccaacc ggcggccggc gccgagccgg gagcgaggcg
       61 tccgccagcc ggcagcggca tgtcgcggct ccacgtgtag ccgacggaga ttggaccctt
      121 cgggaacgcg gctggcatga gcgacacggc atcggcgccg gcggacagat cgtcgccatt
      181 cgcgtcgacg agcgccaccg tgccatccgg cgcgatgcgg agcagcatgg tacgaccctc
      241 gaccgcccgc cgcgcctcgt cgctcagcgc ttcgtcgctc cccgttaggc gaaccgagtc
      301 cgtgatcgct cgcacgtacg tgccggccgg cgcgctccgc tcgatgatcg cccgcgaaaa
      361 tacgtgtagc gacgtcgtga tcgtcgccag ggacggcgtg ccattggcgc gccgagtgcc
      421 cgttagctcc gtctgctggt cgaggcgcag gtgcagggtg tcgccgacgt gcgggtgaat
      481 ctgcaacaga atgccctgcg ctccgctggt cgcgctcctc gtcaggcaca tgacgacgcc
      541 gagcagaaat ggcgtccggg acatcagcgc ccgtccgagc atgatcgtca aaacccgtag
      601 ggatcgtcgc tcggcgccga gcgaatcggg gtgaccatcg gccggggtgc cggcgtcgct
      661 gggacgatcg gcgcgagctg tgacttgaag aaacgcttca catccgcggg ccgctcgcga
      721 attgtcgcat tgcgcgcgcg gatcacctcg tccggctcgc gaatgccgat cgcatcctgc
      781 acccagttca gggtgagggt cgggtcgagt tgccggaggg aacgcagcac cgccgcctcg
      841 atggtgacat cctgcgcctg cgggaatcgc tcggcgccat cgcgcccgcg catgacggcg
      901 gggcgcggga accgaacgaa aattggttgt gtgaagtgcg gatgccgcac catgagctgt
      961 cccttctcca acgttgcgag ctttgtcttc acggccgggc tgagcaccgc atagcccggg
     1021 gtcgccagct cgtccgcatc catgcgtccg tacagcgccg tccccgagtt gccgaccacc
     1081 cgacgatgga cctgcgatcg aaattgttga gcactgaaga gcacgagtcc gagataccgg
     1141 ccccgctcgg cgagatcgag gagcatcttg cggacgtacg tgtcctgtcc atcgctcggc
     1201 gcgtatttgt tgagctcgtc cacgaagacg acgacgtggt tcaccccgag atcgcgcttc
     1261 tccagatgct cgcgcagctt ggtgaccacc cgcgcgaata ccagatcctg cgcgtcctct
     1321 tcgagcgacg cgacgtcgat cacatacacc gttctatcct gaaagctccc gaacggcagg
     1381 tcgccaacgt tcccgtcgtc ggtcacgaga ccggcgcatc gggtcgagat gttcgagagg
     1441 cggttccgca ccttgcgaat cgtggcgatg tgatgggtac gcca
//
LOCUS       DASUCJ010000934         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_346279, whole genome shotgun sequence.
ACCESSION   DASUCJ010000934 DASUCJ010000000
VERSION     DASUCJ010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346279"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1144
                     /locus_tag="VFU90_11150"
     CDS             116..1144
                     /locus_tag="VFU90_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5480384.1"
                     /translation="MTGPRVGLDARLTKQMSVGMKTYAAELTARLPRVAPDFTFVPFT
                     RGTNFGWSEQVALPLAIGRARLDLVHFLSLYAPLLSPVRSVVTIHDLIHLRFPQYFKA
                     KVRPYYQTAVRWACVRARRVITDDERTIGDLREFLGVDPRKVRVVPLGVDERFLNAGT
                     AERVPSPRRYFLYAGNHRRHKDLPTLFAAWSALGDETPIDLYVTGPDDFGGELQRRRT
                     PSRAIVALGDVSAAQLAGYYAGALALVTPALREGFGLPMLEAMASGCPVIACESAVPR
                     VLAPAALTFAASDRERLAARMRSVASDEGLRDRLVKLGLEIARSLTWDRCAQATADVY
                     REVLEDAC"
     gene            1138..>1643
                     /locus_tag="VFU90_11155"
     CDS             1138..>1643
                     /locus_tag="VFU90_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5480385.1"
                     /translation="MLTIAALLAAAVAAPAAKSSPAPKPPSAITIVINGNKLPIDPPP
                     RVEHRLLFVPVRRTLDALGLPFDRSGRRVTTQVGSKSVVLTVGSRIAEVDGDRVELDA
                     PAAEIKSVLYAPLRFFTDVLGAQARYDPRSKTVTIVAQLIGTGNAGIEKTGAGYTREG
                     TVAAVDVLS"
BASE COUNT          245 a          542 c          571 g          285 t
ORIGIN      
        1 ggtttccttg gctgctcttt cgaaaaggag ccgtgctcga cggtcctcgc ggaatctacg
       61 tcgcgtacaa gtcggcgacg tatccggcgg tggtagcgtg gaaagcgcgc cggcgatgac
      121 gggcccgcgc gtcgggctcg acgcacgcct taccaagcaa atgtcggtgg gcatgaagac
      181 gtacgcagcc gagctgaccg cgcggcttcc gcgagtggca ccggacttta ccttcgttcc
      241 gttcacccgc ggaacgaatt tcggttggag cgaacaggtg gcgctcccgc ttgcgatcgg
      301 ccgcgcgcgc ctcgatctcg tgcactttct ctcgctgtac gcgccgctgc tttcgccggt
      361 gcgctcggtc gtgacgatcc acgatctcat ccacctgcgt tttccgcaat atttcaaggc
      421 gaaggtacgg ccgtactatc aaacggcggt ccgctgggcg tgtgtgcgcg cgcgccgggt
      481 gatcaccgac gacgagcgaa cgatcggcga tctgcgggag tttctcggcg tcgacccgcg
      541 caaagtgcgc gtcgttccgc tcggcgtcga cgagcgtttt ctgaatgccg gaacggccga
      601 gcgggtgccg tcgccgcggc ggtatttctt gtacgccggg aatcaccgcc ggcacaagga
      661 cctgccgacc ctgtttgccg cctggtcggc gctcggcgac gagacgccga tcgacctcta
      721 cgtcaccggt ccggacgatt tcggcggcga gctgcagcgg cggcgcacgc cgtcgcgcgc
      781 gatcgtcgcc ctgggagacg tatcggccgc gcagctcgcc gggtactacg cgggtgcgct
      841 cgcgctcgtc acgccggcgc tgcgcgaagg tttcggactg ccgatgctgg aggcgatggc
      901 gtcgggctgc ccggtaatcg cgtgcgaaag cgcggtcccg cgcgtcctcg cacccgcggc
      961 gctgacgttt gctgcaagtg accgggagcg attagcggcg cgcatgcgca gcgtcgcctc
     1021 cgacgagggt ctgcgcgacc ggctcgtcaa gttagggctc gagatcgcgc gttcgctcac
     1081 ctgggaccgg tgcgcgcagg caaccgcgga cgtctatcgt gaggtcttgg aggacgcgtg
     1141 ttaacgatcg cagcgctgct cgccgcagcc gttgccgcgc cggcggcaaa gtcttcgccc
     1201 gcgcccaagc cgcccagcgc gatcacgatc gtgatcaacg gcaacaagct tccgatcgat
     1261 ccaccgccgc gcgtcgagca ccggctgctg ttcgtgcccg tgcggcgcac gctggacgcg
     1321 ctcggcctgc cgttcgaccg ctccggaagg cgcgtaacca cccaggtcgg ctcgaaatcg
     1381 gtcgtgttga cggtcgggag ccggattgcc gaagtcgacg gcgaccgcgt cgagctggac
     1441 gctccggcgg cggaaatcaa gagcgtcttg tacgcgccgt tgcgtttctt taccgacgtt
     1501 ctcggcgcgc aggcgcgcta cgatccccgc agcaagacgg tcacgatcgt cgcgcagctg
     1561 atcggcacgg gcaacgcggg gatcgaaaag acgggagcgg ggtacacgcg ggagggaacg
     1621 gtcgcggcgg tcgacgtgct ttc
//
LOCUS       DASUCJ010000935         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_10054, whole genome shotgun sequence.
ACCESSION   DASUCJ010000935 DASUCJ010000000
VERSION     DASUCJ010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10054"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="ribB"
                     /locus_tag="VFU90_11160"
     CDS             complement(<1..681)
                     /gene="ribB"
                     /locus_tag="VFU90_11160"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEU5480386.1"
                     /translation="MSFVEVVSKIEEIIDEARNGRMFILVDDEDRENEGDLVIPAQMA
                     TPDAINFMAKHGRGLICLALTRQRVEQLDLKLMSQANASRLQTAFTVSIEARDGVTTG
                     ISASDRARTVSVAIDPSKGADDIVSPGHVFPLMARDGGVLVRTGHTEAAVDIARLAGL
                     NPSGVICEIMNDDGTMARLPDLVKFAQLHGLKVGTIADLIAYRRKHDHFVHRAVDTNF
                     ESHNGGAWK"
     gene            complement(678..>1066)
                     /locus_tag="VFU90_11165"
     CDS             complement(678..>1066)
                     /locus_tag="VFU90_11165"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5480387.1"
                     /translation="TVGSWRAGTRINLERALKMGDELGGHLLTGHVDTVATLTERRPE
                     GDSLRLTFSVPEPFDRAIASKGAVAIDGVSLTVNEVDGNRFGVNIIPHTQRETTLGAL
                     QPGDRVNLEIDLIARYLARLVGKEIA"
BASE COUNT          171 a          334 c          367 g          194 t
ORIGIN      
        1 cttccacgcg ccgccgttgt ggctctcgaa atttgtgtcg acggcacggt gcacgaaatg
       61 gtcgtgcttg cggcgatagg cgatcaggtc cgcgatggtt ccgaccttga gaccgtggag
      121 ctgggcgaat ttcacgaggt ccggcagccg cgccatggtg ccgtcgtcgt tcatgatctc
      181 gcagatcacg cccgagggat tgaggccggc aagccgcgcg atatcgaccg cggcctcggt
      241 gtggccggtg cgcaccagca cgccgccatc gcgcgccatc agcggaaaga catggcccgg
      301 gctgacgatg tcgtcggcgc ccttgctcgg gtcgatggcg accgagacgg tgcgcgcgcg
      361 gtcggacgcg gaaatgccgg tggtgacgcc gtcgcgcgcc tcgatggaga cggtgaacgc
      421 ggtctgaagc cgcgaggcgt tggcctgcga catcagcttg agatcgagct gttccacgcg
      481 ctgtctggtc agggccaggc aaatcaggcc gcggccgtgt ttcgccatga aattgatcgc
      541 gtcgggcgtc gccatctgcg ccgggatgac gaggtcgcct tcgttctccc ggtcttcgtc
      601 gtcgaccaga atgaacatgc ggccgttgcg cgcctcgtcg atgatctctt cgatcttcga
      661 gacgacttcg acaaaactca tgcgatctcc ttgccgacga gacgcgcgag atagcgcgcg
      721 atcaggtcga tttcgagatt gacgcggtcg cccggctgga gcgccccgag cgtggtctcg
      781 cgttgcgtat gcgggatgat attgacgccg aaacggttgc cgtcgacctc gttcacggtc
      841 aacgacacgc cgtcgatggc gacggcgccc ttcgacgcga tggcgcgatc gaacggctcg
      901 ggcacggaaa acgtcaggcg aagggagtcg ccttcgggcc ggcgctcggt cagcgtcgcg
      961 accgtatcga catggccggt cagaagatgc ccgcccagtt cgtcccccat cttcagggcg
     1021 cgctcgaggt tgatgcgcgt gccggcgcgc cacgatccca cggtgg
//
LOCUS       DASUCJ010000936         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1416155, whole genome shotgun sequence.
ACCESSION   DASUCJ010000936 DASUCJ010000000
VERSION     DASUCJ010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416155"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..>1164)
                     /locus_tag="VFU90_11170"
     CDS             complement(269..>1164)
                     /locus_tag="VFU90_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5480388.1"
                     /translation="DLENIRYSFLNVYQIEPNVSALIEKARKHGRVSVAMAYADFNEH
                     PQWVRRGLDVAGIAARDIPVRRFLRDGQERLKSSADLHMLMDIMETALDRPQVSTYVI
                     MTGDSDYIRVITWIKNRFDKRVIVSGVPGTISGDLVAAAGEADHLEAGSGVSSESLMR
                     TVQAIVLMVKSNLPPTGFWTVKLIDQWSRDNRNGVPGTDPDKSNAISYMLRNGLLRRY
                     KTVTDIREVTISELDETNDAVRGYVVGAGFELDPLPVRCARCCVRNEIDVVICVGCGC
                     AFEMTAEPAGAAEGTTEPAGT"
BASE COUNT          165 a          417 c          366 g          216 t
ORIGIN      
        1 tcggcaattc gtcccgcgcc gacgcagtgg gcacccgtcc ccgccgcccg cgcaagttcg
       61 agcaatagcg cggcgcccgt catgtagggg cgggggttgc cccgcccgca cggttcgtgc
      121 ggtcgatgcc cgttgtgcgc ccggtgcccc ttcgtgcgaa tgtgcccgtg gatgcgcccc
      181 gtggcgccgg ctgttcagcc cgcgcaagat caacgacggg gcctatccgg ccccgtcgtt
      241 gcggtagtcc ctggttcttc tctgcgatct acgtgcccgc cggctccgtc gttccttcgg
      301 ccgcgcccgc cggctctgcc gtcatctcga aggcgcaccc gcaaccaacg catatcacca
      361 cgtcgatctc gttccgcacg cagcagcgcg cgcaccgcac cggcaacggg tcgagctcga
      421 acccggcccc gacgacgtag ccgcgaacgg cgtcgttcgt ctcatccagc tcgctgatcg
      481 tgacttcgcg gatgtcggtg accgtcttgt accggcgcag cagcccgttc cgcagcatgt
      541 acgagatcgc gttcgacttg tccgggtccg tgcccggcac gccgttgcgg ttgtcgcgcg
      601 accactggtc gatgagcttc acggtccaga acccggtcgg cggcaggttc gacttcacca
      661 tcagcacgat cgcctgcacc gtgcgcatga gcgactcgct gctgacgccg ctccccgctt
      721 ccaggtggtc cgcttcgccg gcggccgcga cgaggtcgcc gctgatcgtc cccggcacgc
      781 cggacacgat cacccgcttg tcgaagcggt tcttgatcca cgtgatcacg cggatgtagt
      841 cggagtcgcc ggtcatgatc acgtacgtgc tcacctgcgg ccggtcgagc gccgtttcca
      901 tgatgtccat cagcatgtgg aggtcggccg aagacttgag ccgctcctgc ccgtcgcgca
      961 ggaagcggcg caccgggatg tcgcgcgcgg caatgccggc gacgtcgagc ccgcgccgga
     1021 cccactgcgg atgttcgttg aagtccgcgt acgccatcgc gacggagacg cggccgtgct
     1081 tccttgcctt ctcgatcagc gctgacacgt tcggttcaat ctggtagacg ttcaaaaagc
     1141 tgtaacggat gttctcaagg tcga
//
LOCUS       DASUCJ010000937         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_835144, whole genome shotgun sequence.
ACCESSION   DASUCJ010000937 DASUCJ010000000
VERSION     DASUCJ010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835144"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1012)
                     /locus_tag="VFU90_11175"
     CDS             complement(44..1012)
                     /locus_tag="VFU90_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5480389.1"
                     /translation="MKASLSLDLDNKWSYMKTHGDPAWESLPSYLDILVPRVLNGLEK
                     RGLRITFFIVGQDAAQPGHAEVLREIARRGHEIGNHSHVHEPWMHRRSETAIDDELAR
                     AEESIERATGERPRGFRGPGFTRSEKILETLVRRGYLYDASSLPTFIGPLARAYYFRN
                     AKLSEAARREREDLFGSFADGFRPNRQHAIAVANGSIAEIPVTTFPLLRVPIHISYVL
                     YLATIAPALALAYFRTALLCCKLTATEPSILLHPLDFLSGDECAGLGFFPAMGMEREL
                     KDRIVLSAIDALAAGFDVRPMRETVKVPAAQAASAVQEVARSYSSR"
     gene            complement(1009..2343)
                     /locus_tag="VFU90_11180"
     CDS             complement(1009..2343)
                     /locus_tag="VFU90_11180"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5480390.1"
                     /translation="MSRFGIVGGGMLGMTLAWNLSKAGHEVTIFEGAERCGGLAAPWE
                     LGDAVWDRHYHVTLGSDTALRALLRELDVESEINWVKVGTGFFVDGAMHPFSSVFDYL
                     RFPPLSPVQKLRLAATILKASRIRAWEPLEELTAVEWLTQECGRATVERIWLPLLRAK
                     LGPYATRASAAFIWAIIARMYAARANGAKSESFGYVRRGYDRILRRFEERLAERGVEI
                     RTRHAIERVGSDGSGGVAIVTADGPHRFDRAVVTMAAPLAGRLCPELTEHERALCAGV
                     EYQGIVCASILSDVGLTPYYITNIADASVPFTAVIEMTALVDKQEFGGKSLVYLPKYV
                     ASDDPALFRSDEEIASSFIPALQRMHPQFDSRSIRAFRVSRVRYVFPIPTVGYSRRLP
                     PVRTSVPGLFMAGSAHIVNGTLNVNETVKLANGLVPALLAEEPAAPQRLATV"
BASE COUNT          439 a          793 c          770 g          403 t
ORIGIN      
        1 aaccgtgcgg gcgcagtcgc cggttcggtc gcgcgacgcg acctcagcgc gacgagtagc
       61 ttcgcgcgac ctcctggacc gcactcgcag cttgcgcagc cgggaccttg acggtctctc
      121 gcatgggccg aacgtcgaat cccgccgcca gcgcgtcgat cgccgacaag acgatgcgat
      181 ccttcaactc tcgctccatc cccatcgccg ggaagaatcc caggcccgcg cactcgtcgc
      241 cgctcagaaa gtccagcggg tgcagtagga tcgacggctc cgtggcagtg agcttgcagc
      301 atagcaaagc ggtacggaag tacgcgagcg ctaacgccgg cgcgatcgtc gcgagataga
      361 gcacgtagct aatatgaata ggaacgcgca gcagcggaaa ggtcgtcacc ggaatctcgg
      421 cgatcgaccc gttggccacg gcgatcgcat gctggcgatt cggccggaat ccatccgcaa
      481 agctgccaaa cagatcttcg cgctcgcgcc gagccgcttc gctcagtttg gcgttgcgga
      541 aatagtacgc gcgcgcgagc gggccgatga acgtcggcag cgacgaggcg tcgtagagat
      601 agccgcgccg caccagcgtc tcgaggatct tttccgaacg ggtgaatccc gggccgcgaa
      661 agccgcgcgg ccgttcgccg gtcgcccgtt cgatgctttc ttccgcccgc gccagctcgt
      721 cgtcgatggc ggtttcgctg cggcgatgca tccacggctc gtgcacatgc gaatggttgc
      781 cgatctcgtg cccgcggcgc gcgatttccc gcagcacttc ggcgtgcccc ggctgcgccg
      841 catcctgacc gacgatgaag aacgtaatcc gcagaccccg cttttccagt ccgttgagaa
      901 cgcgcggaac caggatatcg aggtaggagg gaagcgattc ccaggccgga tccccgtgcg
      961 tcttcatgta cgaccatttg ttgtcgagat ccagcgagag cgaggctttc atacggtagc
     1021 caatcgttga ggtgcggccg gttcttcggc caggagcgcc ggcacgaggc cgtttgcgag
     1081 tttgacggtt tcgttgacgt tgagcgttcc gttgacgatg tgcgcgctcc cggccatgaa
     1141 cagccccggg accgacgtgc gtaccggcgg gagccggcgc gaatatccga ccgtgggaat
     1201 cgggaaaacg tatcggacgc gcgacacgcg aaatgcccga atcgaacgcg aatcgaattg
     1261 cgggtgcatc cgctggagtg cgggaatgaa gctcgatgcg atctcttcgt cgcttcgaaa
     1321 gagcgccgga tcgtcggagg cgacgtattt ggggagatag acgagcgact ttccgccgaa
     1381 ctcctgctta tcgaccagcg ccgtcatttc gatcaccgcc gtgaacggaa ccgacgcgtc
     1441 cgcgatattc gtgatgtagt acggcgtcaa tcccacgtcg ctcaagatcg acgcgcagac
     1501 gatcccttgg tattcgacgc cggcgcacaa cgcgcgctcg tgctccgtca gttccgggca
     1561 cagacgcccg gccagcggcg ccgccatcgt tacgaccgcg cgatcgaagc gatgggggcc
     1621 gtcggcggtc acgatggcga cgccgccgct accgtccgat ccgacgcgtt cgatcgcgtg
     1681 gcgcgtgcga atctcgacgc cccgctccgc gaggcgttcc tcgaaccgcc ggaggatgcg
     1741 gtcgtacccc ctgcgcacgt agccgaacga ctcgctcttg gctccgttgg cgcgggccgc
     1801 gtacatgcgg gcgatgatcg cccaaataaa ggccgccgat gcacgggtcg cgtacgggcc
     1861 gagcttcgcg cgcagcagcg ggagccaaat gcgctcgacc gtggcgcgtc cgcactcctg
     1921 cgtgagccac tccaccgcag tcagctcctc gagcggctcc caggcgcgga tgcgcgacgc
     1981 cttgaggatc gtcgccgcca ggcgcagctt ctgtaccggt gaaagcggtg ggaaccgcaa
     2041 atagtcgaaa acgctcgaaa acggatgcat cgcgccgtcg acgaaaaacc ccgtcccgac
     2101 tttcacccaa tttatttcgg attcgacgtc gagttcgcgg agcagggcgc gcagagccgt
     2161 gtcgctgccg agcgtgacgt gatagtgacg gtcccaaacc gcgtcgccga gttcccacgg
     2221 ggcggcaagc ccgccgcagc gctcggctcc ttcgaaaatc gtgacctcgt gcccagcttt
     2281 cgagagattc cacgccagcg tcatgccgag catgccgccg ccgacgatcc cgaaacggct
     2341 catcgcgcga ggaccgtttg tgaaagcttg gcgccgtcga actcgtagga cgacccgcac
     2401 ggccc
//
LOCUS       DASUCJ010000938         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_682571, whole genome shotgun sequence.
ACCESSION   DASUCJ010000938 DASUCJ010000000
VERSION     DASUCJ010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682571"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFU90_11185"
     CDS             complement(<1..296)
                     /locus_tag="VFU90_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5480391.1"
                     /translation="MVGRPAVAMTGGDRTFRAALRAWLAQHPPPAADDVETLRNWQQT
                     LHSGGWVGVHWPAEYGGRGASPAQVAIYNEELARAGAPPLLGRVGVTLVGPTLMT"
     gene            complement(275..>1020)
                     /locus_tag="VFU90_11190"
     CDS             complement(275..>1020)
                     /locus_tag="VFU90_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5480392.1"
                     /translation="MGQAADGLKKVLLELGGKSPNIVFAGADVAKFAASAAFGFTIHA
                     GQGCALPTRILAERSIYDELVERMADALGKIKVGDPTDASVGMGPLIRESQRARVEQY
                     VAAGTAEGARVACGGRRPESLTRGFFYEPTLFADVKSSMRVAQEEIFGPVGVASPFDN
                     EEEAIAIANDTRYGLAASVWHPDPPRAFELAKRIHAGTVSINGGGGGPNPWGPFGGYK
                     HSGIGREFGDYGLLEYTQLKTVAWSAGRP"
BASE COUNT          151 a          372 c          334 g          163 t
ORIGIN      
        1 gtcatcaacg tcggcccgac gagagtgacg ccgacgcgtc cgagcaacgg tggcgcgccc
       61 gcccgcgcaa gctcttcgtt gtagatcgcg acctgcgccg gggacgcccc gcgcccgccg
      121 tactcggccg gccagtgcac accgacccac ccgcccgagt gcagcgtttg ctgccagttc
      181 cgcagtgtct cgacgtcgtc cgccgccggc ggtggatgct gtgccagcca cgcgcgcagc
      241 gcggcgcgaa acgtgcggtc gccgccggtc atcgctacgg ccggccggcc gaccacgcca
      301 ccgtcttgag ctgcgtgtat tcgagcaggc cgtagtcgcc gaactcgcgg ccgatgccgg
      361 agtgcttgta gcccccgaag ggtccccacg ggttgggacc gccaccgccg ccgttgatgc
      421 tcaccgtccc cgcatgaatg cgcttggcga gctcgaacgc gcgcggcgga tcgggatgcc
      481 acacggatgc cgcaaggccg tatcgcgtgt cgttcgcgat cgcgatggct tcttcttcgt
      541 tgtcgaacgg acttgcgaca cccacgggac cgaagatctc ttcttgcgcg acgcgcatcg
      601 agctcttcac gtcggcgaac aacgtcggct cgtagaagaa gccgcgcgtc aagctctcgg
      661 gccggcggcc gccgcacgcg acgcgcgcgc cttcggcggt gccggcggcg acgtactgct
      721 ccacgcgcgc gcgttgcgat tcgcggatca gcggccccat gccgacggat gcatcggtcg
      781 gatcaccgac cttgatcttt ccgagtgcgt cggccatgcg ctcgacgagc tcgtcgtaga
      841 tgctgcgctc ggcgaggatt cgcgtcggca acgcgcaccc ttgcccggcg tggatcgtga
      901 aaccgaacgc cgccgaggct gcgaacttcg caacgtcggc acccgcgaag acgatgttgg
      961 gcgacttgcc accgagctcg agcaacacct tcttcagccc gtccgcggcc tgccccatga
//
LOCUS       DASUCJ010000939         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_605817, whole genome shotgun sequence.
ACCESSION   DASUCJ010000939 DASUCJ010000000
VERSION     DASUCJ010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605817"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VFU90_11195"
     CDS             complement(<1..1425)
                     /locus_tag="VFU90_11195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5480393.1"
                     /translation="MNARIWTGDARRPWADALAATGERLTAVGTSAEVKKLVGASTRV
                     IDAKGMMVVPGFIDSHVHFISGGFGLSSVQLRDATTPAEFIRRIKAYAATLPAGAWIT
                     EGNWDHELWGGELPRRDWIDSVTPDNPVWINRLDGHMGLANSAALRAAGVEASTKEVD
                     GGTIVRDAAGAPTGVLKDNAQSLIDRVVPDPPMEVVDRAVDAAFAYLAGNGVTSVHNM
                     GTWDDLAAFERAQRTGRMRTRIYAVVPLASWERLRDTVAARGRGDAWLHIGGLKGFVD
                     GSLGSHTAAMLEPFTDAPNDTGLFVNTPADLYAWTSGADRAGLQVIVHAIGDRAIRTQ
                     LDIYERVEREDGAKDRRFRIEHLQHIAPSDIPRVASLGVIGSMQPYHAIDDGRWADKV
                     IGPERAKTTYAFRSLLDAGVRLAFGSDWFVAPATPIEGIYAAVTRRTLDDKHPNGWVP
                     EQKITVEDALRAYTSGAAYAEFAEQ"
     gene            complement(1615..>1916)
                     /locus_tag="VFU90_11200"
     CDS             complement(1615..>1916)
                     /locus_tag="VFU90_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480394.1"
                     /translation="VEREHREHERDEQEPEPQIGCDPDHRSFTPPLTSKRKRDHYDTA
                     CVVVVSPEARAIRATQPTGSASCSVLTIGCSGVRAPAGYSPLHRMVHSSCRRVKL"
BASE COUNT          320 a          635 c          638 g          323 t
ORIGIN      
        1 ctgctccgca aactccgcgt aggcggcgcc gctcgtgtag gcacgcagcg cgtcctcgac
       61 ggtgatcttc tgctcgggca cccagccgtt cggatgcttg tcgtcgagcg tgcgccgcgt
      121 gaccgccgcg tagatcccct cgatcggtgt cgcgggcgcg acgaaccagt cgctcccgaa
      181 cgcgagccgc acccccgcgt cgagcaggga gcggaaggcg taggtcgtct tcgcgcgctc
      241 ggggccgatg accttgtccg cccatcgacc atcgtcgatg gcgtgatagg gctgcatgct
      301 gccgatcaca cctaacgatg ccacgcgagg gatgtcgctc ggcgcgatgt gctggaggtg
      361 ctcgattcgg aaacgccggt ccttcgcgcc gtcctcgcgt tcgacccgtt cgtagatgtc
      421 gagctgcgtg cggatcgcgc gatcaccgat ggcgtggacg atcacctgca acccggccct
      481 gtccgcgccc gaggtccagg cgtagagatc cgccggagtg ttgacgaaga ggcccgtgtc
      541 gttaggtgcg tcggtgaacg gttcgagcat cgccgcggtg tgggacccga gcgagccgtc
      601 gacgaatccc ttcaagccac cgatgtgcag ccaggcatcg ccgcgaccgc gcgcggcaac
      661 ggtgtcgcgg agccgctccc acgacgccag cggcaccacg gcgtagattc gcgtcctcat
      721 gcggcccgtg cgctgcgcac gctcgaatgc agccagatcg tcccacgttc ccatgttgtg
      781 caccgacgtg acgccattcc ccgcgagata cgcgaacgcc gcatcgacgg cacggtcgac
      841 gacctccatc ggcggatcgg gcacgacccg atcgatgagg gactgcgcgt tgtccttgag
      901 gacgccggtg ggtgcaccgg cggcgtcgcg cacgatcgtg ccaccgtcca cctcctttgt
      961 gctggcctcg acgccagcgg cgcgcaacgc ggcactgttg gcgaggccca tgtgtccgtc
     1021 gagccggttg atccacaccg ggttgtcagg cgtcaccgaa tcgatccagt cacgtcgggg
     1081 cagctcgccg ccccacagct cgtgatccca attgccttcc gtgatccagg cgcccgccgg
     1141 cagcgtcgcc gcgtacgctt tgatgcgccg tatgaactcc gccggcgtcg tcgcgtcgcg
     1201 gagctgcacg gacgagagac caaagccgcc gctgatgaaa tggacgtgcg agtcgatgaa
     1261 tccaggaacg accatcatgc ctttggcgtc gatgacacgt gtcgacgcac cgacgagctt
     1321 cttcacttcc gcactcgtgc cgaccgccgt gaggcgctcg ccagtagccg caagcgcgtc
     1381 cgcccatgga cggcgtgcat cgccggtcca gatgcgcgcg ttcacgacgg ccaaggtcgg
     1441 ttcgacgaac gaccgaccgg agctcgagga gcgattcgtg cgttgagcgg acatgacact
     1501 cgcgggaaga agggccgcca tcacgacggc gcggcagagg actgacgatg acatgaggcg
     1561 ttttcgtgaa gaaggcggct caacactcgc cgccaaacgc gacgaacgga agggtcagag
     1621 cttgacccgc cggcatgacg agtgcaccat tcgatgcagt ggggagtagc cggccggcgc
     1681 gcggacgccg gaacagccga tcgtcaagac ggagcacgac gcgctcccgg ttggctgagt
     1741 ggcgcggatg gcccgcgctt caggcgagac cactacgaca catgcggtgt cgtagtggtc
     1801 tcgttttctt tttgaggtga gcggaggagt gaacgatcga tgatcgggat cgcaaccaat
     1861 ctgtggttct ggctcctgtt catcgcgttc gtgctcgcga tgctcgcgct cgacct
//
LOCUS       DASUCJ010000940         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_193305, whole genome shotgun sequence.
ACCESSION   DASUCJ010000940 DASUCJ010000000
VERSION     DASUCJ010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193305"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFU90_11205"
     CDS             <1..631
                     /locus_tag="VFU90_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5480395.1"
                     /translation="VLSPFRHGLAYADVVLPIAPFTETAGTFVNCEGRVQSFRGVAAP
                     LGAARPGWKVLRVLGSLLEIEGFDAIDNSESVRDALLAGRVTDGIVAGLSNASRMEIV
                     KPATVAAAVERVADVPIHFADPLVRRAASLQQTADARAPKAHVHPTLLATLGIEEGAQ
                     IRLRQGGGEAVLATEVDRGVPPGVVRVAAAHPSTCGLDGLSGTVTVERA"
     gene            637..>1679
                     /gene="nuoH"
                     /locus_tag="VFU90_11210"
     CDS             637..>1679
                     /gene="nuoH"
                     /locus_tag="VFU90_11210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5480396.1"
                     /translation="MLEPVQAAMGAWWLPVWTIVKVIVIAIPLIIAVAYLTYAERKVI
                     GWMQVRIGPNRVGPLGLLQPFADVLKMLFKEIVVPSGANRSLFFIAPMLSLAPALAAW
                     AVIPFTGTLVLSNINAGLLYILAMSSLGVYGVILAGWASNSKYAFIGCLRSAAQIVSY
                     EIAMGFALVGVLMAANSLNLRDIVAGQEGGAAYWYVWPLLPLFVVYLVAGIAETNRHP
                     FDMAEGESEIVAGFHVEYSGIAFALFFLAEYMNMILISALATIMFLGGWLSPFPILND
                     VSVFGVHPFGDGMLWMAIKWAAVLLIFLWIRATFPRYRYDQIMRLGWKVFLPVTLVWI
                     VFLAVMMQTRWAGL"
BASE COUNT          222 a          556 c          574 g          327 t
ORIGIN      
        1 ggtgctgtcg ccgttccggc acggcctcgc gtacgccgac gtcgtgctgc cgatagcacc
       61 ctttaccgaa accgcgggca ccttcgtcaa ctgcgaagga cgcgtgcagt cgttccgcgg
      121 cgttgcagct ccgctcggcg ccgcgcgtcc ggggtggaag gtgctgcgcg tgctggggtc
      181 gcttctggag atcgaaggct tcgacgccat cgacaactcg gagtcggtgc gcgacgcgct
      241 gctcgcgggt cgcgtgaccg acggcatcgt cgccggtctc tcgaacgcgt cgcggatgga
      301 gatcgtgaaa cccgcgaccg tcgccgcggc ggtcgagcgc gtcgccgacg tgccgatcca
      361 tttcgccgat ccgctggttc gccgtgccgc ttcgctgcag cagaccgccg acgcccgcgc
      421 gccgaaggcg cacgtgcacc cgacgctcct cgcgacgctc ggcatcgagg aaggggcaca
      481 gatccgactg cgccaggggg gcggcgaggc ggtgctcgcg accgaggtcg accgcggtgt
      541 tccgcccggc gtcgtccgcg tggccgccgc gcatccgtcg acctgcgggc tcgacggcct
      601 ttcgggcacg gtcaccgtgg agcgcgcgtg agcacgatgc tcgaaccggt ccaggccgcg
      661 atgggcgcgt ggtggctgcc cgtgtggacg atcgtcaagg tgatcgtgat cgcgattccg
      721 ctgatcatcg ccgtcgccta tctcacctac gccgagcgca aggtgatcgg ctggatgcag
      781 gtgcgtatcg ggcccaaccg cgtcgggccg ctcgggctgc tgcagccgtt cgccgacgtg
      841 ctgaagatgc tgttcaagga gatcgtcgtc ccgtctggcg cgaaccgctc gctcttcttc
      901 atcgcgccga tgctctcgtt ggcgcccgcg ctcgccgcgt gggcggtgat tccattcacc
      961 ggcacgctgg tgctgtcgaa catcaacgcc gggctcctct acatcctcgc gatgagctcg
     1021 cttggcgtgt acggagtgat cctcgccgga tgggcgtcga actcgaagta cgcattcatc
     1081 ggctgcctgc gctcggccgc gcagattgtt tcgtacgaga tcgcgatggg cttcgcgctg
     1141 gtcggcgtgc tgatggcggc gaacagcctc aacctgcgcg acatcgtcgc aggacaggaa
     1201 gggggggcgg cgtactggta cgtctggccg ctgttgccgc tcttcgtcgt ctacctggtg
     1261 gcaggcatcg ccgagaccaa ccgccatccg ttcgacatgg cggaaggcga gtcggagatt
     1321 gtcgccggct tccacgtcga gtattcgggc atcgccttcg cgctgttctt cctcgccgaa
     1381 tacatgaaca tgatcctgat ctcggcgctc gcgacgatca tgttcctcgg cggctggctg
     1441 tcgccgttcc cgatcctgaa cgacgtgtcg gtcttcggcg tgcacccgtt cggcgacggc
     1501 atgctgtgga tggcgatcaa gtgggccgcc gtgctgctga tcttcctgtg gatccgcgcg
     1561 acgtttccgc gctaccgcta cgaccagatc atgcggctcg gctggaaggt gttccttccg
     1621 gtgacgctcg tttggatcgt gttcctcgcc gtcatgatgc agacgcgctg ggcgggtct
//
LOCUS       DASUCJ010000941         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_453377, whole genome shotgun sequence.
ACCESSION   DASUCJ010000941 DASUCJ010000000
VERSION     DASUCJ010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453377"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFU90_11215"
     CDS             complement(<1..781)
                     /locus_tag="VFU90_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEU5480397.1"
                     /translation="MTAFYLYDDAQARAIAPFALTRPLGEMRAGAELIRRRWEMAFGV
                     PAAGTVAAPHLECFDEPGAPPPAAGSLPAHAILVNSRCVVPLGWKARAGDVWTCAGEI
                     AAVRLARDTPVSELAHGDAPLQSLGTVGSRVEAIAGRWVTAVWSFIADLLAQLREDIA
                     VLGPLAAVTQPEGSIVIGAHQIYVEPDAMIEPGVCFDVTAGPVLVRRGAVVRAFTRLV
                     GPCVVGAAATVLGDRINGCAIGDGAVVRGELSESIILGQANK"
     gene            complement(778..1812)
                     /locus_tag="VFU90_11220"
     CDS             complement(778..1812)
                     /locus_tag="VFU90_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5480398.1"
                     /translation="MSRWAVILAGGSGTRFWPLSTPERPKQLLPLATSEPLLVDAIRR
                     LGPLVPVERTLIITSAALADAVHAVVPALPRANIVGEPRAAGTGPALAWAAADIARRD
                     ADDPVMLCVHADWSIRNDDGFRETLSRAAAVAERHGALVTVGIVPERPDPGFGYIQPG
                     EHVTGARRVARFAEKPDRATAERMVSEGYLWNSGIFVWRAQDFLAEIASHTPEMRSAL
                     KHASAGDMDTFYSTVTPVTVDEGVLERSRNVLVIPGEFGWDDVGTWAALRRVHERDAA
                     GNAVRGDVHLVRARDCVVYAEDQTVVLYGVADLVVVARDGLTVVTTTDGAADLKSLID
                     ALPAHVRDRR"
     gene            complement(1809..2207)
                     /locus_tag="VFU90_11225"
     CDS             complement(1809..2207)
                     /locus_tag="VFU90_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5480399.1"
                     /translation="MADDEPHIGRIIKMKLEQGPFAVTLAYDGREALDALAHDSDVAL
                     VMLDLMMPHLSGLDVLARMRSDTRWQRLPCIILTAAGQEQHHREAMALGASEFLTKPF
                     SPKKLYARAADLLGVGDDVETSGELRTPES"
     gene            complement(2242..2595)
                     /locus_tag="VFU90_11230"
     CDS             complement(2242..2595)
                     /locus_tag="VFU90_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5480400.1"
                     /translation="MPTIRDLVAALRQRDGVEAAVVLGRDGLLIDSQTEPTVDAERVA
                     ALIPSIVAAADEYGSHDGRGSLTTAVLEFAQGIAIVSVLNNDAILVVLAKATTNLAPL
                     LFELRRNRENIAALV"
     gene            2778..>3220
                     /locus_tag="VFU90_11235"
     CDS             2778..>3220
                     /locus_tag="VFU90_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480401.1"
                     /translation="MLAASACGNPFAVHATVDVITDTLAVYGLTQAPTNAPTALNTFG
                     PQLVTTSPTTHYDIVFDIRPDSLGNPQAYAEPPRAVASFGSAGFIVDSTQAFDAITFA
                     PNVSYNDSTVVPLNPGTILVVQAASSGCTAQLVTSQMFIYSKIVI"
BASE COUNT          536 a         1127 c         1068 g          489 t
ORIGIN      
        1 acttgttggc ctggccgagg atgatcgact cgctcagctc gccgcggacc accgcgccgt
       61 cgccgatggc gcagccgttg atgcggtcgc cgagcaccgt cgcggcggcg cccacgacgc
      121 acggaccgac caggcgcgtg aacgcgcgca cgaccgcgcc gcggcgcacg agcaccggac
      181 cggccgtcac gtcgaagcac acgccgggct cgatcatcgc gtcgggctcg acgtagatct
      241 ggtgcgcgcc gatgacgatg gaaccctcgg gctgcgtgac agcggccagg gggcccagca
      301 cggcgatgtc ctcgcgcagt tgggcgagga ggtcggcgat gaagctccac accgcggtca
      361 cccaccggcc ggcaatggcc tcgacgcgcg accccaccgt gcccaacgat tggagcggcg
      421 cgtcgccgtg cgcgagctcg gacacgggcg tgtcgcgggc gaggcgtaca gcggcgatct
      481 cgccggcgca cgtccagacg tcgccggcgc gcgctttcca acctaacgga acgacgcagc
      541 ggctgttgac caggatcgcg tgcgcgggca gggagccggc ggccggcggc ggcgcgccgg
      601 gctcgtcgaa gcactcgagg tgcggcgccg ccaccgtgcc cgcggccggg acgccgaacg
      661 ccatctccca ccggcgccga atgagctcgg cgccggcgcg catttcgcct aacggacggg
      721 tgagcgcgaa cggcgcgatg gcgcgcgcct gggcatcgtc gtacaggtag aaggcggtca
      781 ccgccggtcc cgcacgtgcg ccggcagcgc gtcgatcagc gacttgagat cggccgcgcc
      841 gtcggtggtc gtcacgaccg tgagaccgtc gcgcgccacg accacgaggt ccgcgactcc
      901 atagagcacc accgtttgat cttccgcgta gacgacgcag tcccgcgccc gcacgaggtg
      961 cacatcgccg cgcaccgcat tgccggcagc atcgcgctcg tgcacgcgcc gcagcgccgc
     1021 ccaggtgccg acatcgtccc atccgaattc gccgggaatg accagcacgt tacggctgcg
     1081 ctcgagcacg ccttcgtcca cggtcaccgg cgtgaccgta gaataaaagg tgtccatgtc
     1141 gcccgcggac gcgtgcttca gcgccgacct catctccggt gtgtgcgagg cgatctcggc
     1201 cagaaaatcc tgggcgcgcc acacgaagat gccggagttc cagagatacc cctcggacac
     1261 catccgctcg gccgtcgcgc gatcgggttt ctccgcgaat cgcgcgacgc gccgcgcacc
     1321 cgtgacgtgc tcgccgggct gaatgtatcc gaaacccgga tcaggccgct cgggaacgat
     1381 gccgacggtg actaacgcac cgtggcgttc ggcgaccgcc gccgcgcgcg acagcgtctc
     1441 gcgaaatccg tcgtcgttgc gaatcgacca gtcggcgtgt acgcagagca tcaccggatc
     1501 atcggcgtcg cgccgcgcaa tgtcggccgc cgcccacgcg agcgccggcc ccgtgcccgc
     1561 ggcacgcggc tcacccacga tgttcgcgcg gggcagggca ggcaccaccg cgtgaacggc
     1621 atcggccagg gctgccgatg tgatgatgag cgtgcgctcc accggcacga gcgggcctaa
     1681 ccggcggatc gcgtccacca gcagcggttc actcgtggcg agcggcagca actgcttggg
     1741 acgttcgggc gtgctgagcg gccagaatcg cgtgcccgag ccaccggcca gaatgacggc
     1801 ccaacggctc acgactcggg cgtcctcagc tctccggagg tctcgacgtc gtctcccacg
     1861 ccgagcagat ccgcggcgcg tgcatacaac tttttggggc tgaacggctt ggtgaggaac
     1921 tcactcgcgc cgagcgccat tgcttctctg tgatgttgtt cctggccggc cgcggtgagg
     1981 atgatgcagg gcaggcgctg ccagcgcgta tccgagcgca tgcgcgccag cacatcgagg
     2041 ccggacagat gcggcatcat gagatcgagc atcacgagtg cgacgtcgct gtcgtgcgcg
     2101 agcgcatcca acgcttcgcg tccgtcgtag gcgagcgtga cggcgaacgg cccctgctcg
     2161 agcttcatct tgatgatgcg gccgatgtgc ggctcgtcgt ccgccaccaa cacgtgaagc
     2221 cgttcggctt cgacgggcat ctcagacaag cgcggcgatg ttctcgcgat tgcgccgcag
     2281 ctcgaacaac agaggcgcca ggttcgtggt ggccttggcg aggacgacca gaatggcgtc
     2341 gttgttgagc accgagacga tcgcgatgcc ttgcgcgaac tcgagcacgg cagtggtgag
     2401 cgagccgcgt ccgtcgtgcg agccatattc gtcggcagcg gcgacgatgg acgggatcag
     2461 cgccgcgacg cgctccgcgt ctacggttgg ctccgtttgg ctgtcgatga gcaaaccgtc
     2521 gcgacccaac acaacggctg cctcgacacc atcgcgttgg cgcagtgcgg ccaccaaatc
     2581 gcgaatcgta ggcatcggca ctccggcttc gagtgctgcg aagctagacc cggatgtgtg
     2641 tgaagtcaag cacggagaat aacttgaaac cgcaccgccg cgtcggtatc gttccgccat
     2701 ccaccacgat gcttagttcc ccattgcaca gccacatgcc ggccagatcg ctggtcttga
     2761 cctttggcgc tcttgccgtg ctcgcggcgt ccgcgtgcgg caatccgttc gccgtccacg
     2821 ccacggttga cgtcatcacc gacacgctcg ccgtgtacgg tttgacgcag gcgccgacca
     2881 acgcgcccac cgccctcaac acgttcggac cccagctcgt cacgacgagc ccgacgacgc
     2941 actacgacat agtgttcgac attcggccgg acagcctcgg gaatccgcag gcgtacgccg
     3001 agccgccgcg cgccgtggcg tcgttcggca gcgcggggtt catcgtcgat tccacgcagg
     3061 cgttcgatgc gatcacgttc gcgcccaacg tgtcctacaa cgacagcacg gtcgtgccgc
     3121 tcaaccccgg gacgattctg gtcgttcagg ccgcgtcgag cggatgcacg gcccaactgg
     3181 tgacgtcgca gatgttcatt tattccaaga tcgtcatcga
//
LOCUS       DASUCJ010000942         1488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_789366, whole genome shotgun sequence.
ACCESSION   DASUCJ010000942 DASUCJ010000000
VERSION     DASUCJ010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789366"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFU90_11240"
     CDS             <1..575
                     /locus_tag="VFU90_11240"
                     /inference="COORDINATES: protein motif:HMM:NF041730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase H-associated membrane protein"
                     /protein_id="HEU5480402.1"
                     /translation="RLGRFVLRCLAWLPVTFALWYFMAPVLLAPAVWLAQGVSSLAVP
                     DLVRGVAQSGATVTFITRLGAGQAAARGVVTVDVNLLLYSFGLPLYAALTLASRNLRW
                     KRLLVLGYAVLLPFVTWGLVAEFLKNLAITSGAAIASQAGFSSWQREAIALAYQLGAL
                     ILPAVVPVVLWVATQLEPRTNRIVAGSPRT"
     gene            complement(561..>1488)
                     /locus_tag="VFU90_11245"
     CDS             complement(561..>1488)
                     /locus_tag="VFU90_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5480403.1"
                     /translation="DALLPSWMFGVPILGYRGRFDAERAFYLLDKYGVRSSFLFPTAL
                     KLMMKAHPRPKERFDVGLRTIMSAGEAVGTSVFDWAKDALGVTINEMFGQTEMNYIVG
                     NSQSLWPTKPGSMGRPYPGHRVTLLDDDGNEVARGEPGDVAVHRVAPDGTPDPVFFLE
                     YAGNPDATRKKFSGDWCRTGDVATMDDDGYLWYQGRADDVFKAAGYRIGPSEIENCLV
                     RHPAVANAAVVPSPDEVRGNVVKAFVVLAPGHAPSRELEDDLAQHVRRYLAPYEYPKE
                     IEFVDALPMTNTGKVQRKVLRDRERERRLGAG"
BASE COUNT          200 a          499 c          499 g          290 t
ORIGIN      
        1 cgcgcttggg ccgcttcgtc ctccgttgcc ttgcatggct gccggtcacg ttcgcgctgt
       61 ggtatttcat ggcgcccgtg cttctggcgc cggcggtctg gctcgcacag ggcgtctctt
      121 cgctcgcggt gcccgatctc gtgcgcgggg tcgcgcaatc gggcgcgacg gtgaccttca
      181 ttacgcgcct cggcgccggg caggccgcgg cgcgcggtgt tgtcaccgtc gacgtcaatc
      241 tgctcttgta ttcgttcggg ctgccgttgt atgcggcact gaccctcgcg tcgcgcaacc
      301 tgcgctggaa gcgcctgctg gtcctcggct acgcggtgct gctgccgttc gtgacctggg
      361 gactcgtcgc ggaattcctg aagaatctgg cgattacgtc gggtgctgcg atcgcgtcgc
      421 aggcgggatt ctcgagctgg cagcgcgaag cgatcgcgct cgcctatcag ctcggagcgc
      481 tgatcctgcc ggcagtcgtt ccggtggtgc tctgggtcgc tacccagctc gaaccccgga
      541 cgaaccgcat tgttgccgga tcaccccgca cctagcctgc gctcacgctc gcggtcgcgc
      601 agcaccttgc gctgcacctt gccggtgttc gtcatcggca gcgcgtcgac gaactcgatc
      661 tctttcggat attcgtaggg ggcgaggtag cgccgcacat gctgcgcgag atcgtcttcg
      721 agctcgcggc tcggcgcgtg tcccggcgcg agcacgacga atgccttgac gacgttgccg
      781 cgcacttcgt ccggcgacgg gacgacggcc gcattggcga ccgccggatg gcgcacgagg
      841 cagttctcga tctcggacgg gccgatgcgg taaccggccg ccttgaatac gtcgtcggct
      901 cgcccctggt accacaggta gccgtcgtcg tccatcgtcg cgacatcgcc ggtgcggcac
      961 cagtcgccgc tgaacttctt ccgcgtcgcg tcgggattgc cggcgtattc gaggaagaac
     1021 accgggtcgg gcgttccgtc gggtgcgacg cgatgcacgg cgacgtcgcc gggctcaccc
     1081 cgcgcgactt cgttgccgtc gtcgtcgagc agcgtgactc gatggccggg ataggggcgt
     1141 cccatcgatc ccggcttcgt cggccacagc gactgcgagt tgccgacgat gtagttcatc
     1201 tcggtctggc cgaacatctc gttgatcgtg acgccgagcg cgtcctttgc ccaatcgaac
     1261 accgaggttc cgacggcttc gccggcgctc atgatggtgc gcagcccgac gtcgaaacgt
     1321 tccttcggtc gcggatgcgc cttcatcatc agcttgagcg ccgtcgggaa caggaacgag
     1381 ctgcgcacgc cgtacttgtc gagcaggtag aacgcgcgct cggcgtcgaa gcgcccgcgg
     1441 tagccgagga tcggcacgcc gaacatccag gagggcagca gcgcatcc
//
LOCUS       DASUCJ010000943         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1293875, whole genome shotgun sequence.
ACCESSION   DASUCJ010000943 DASUCJ010000000
VERSION     DASUCJ010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293875"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFU90_11250"
     CDS             <1..722
                     /locus_tag="VFU90_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480404.1"
                     /translation="GFLCGAAGGALAIAMTLLTRYRAVADCTIRYDWRRIAVSGLGSA
                     AITLAGALIGSADVILIKHFFDADQAGIYSAASLGGKVLLYFVGFVPTILLPQATERH
                     VRGERTRYALATCVGVLFGISIVGLIALKLFGIVLLHALVGHAFDAAAPLLLPYSVAM
                     MFLALSSVLGAYGIATHRIAFAAPLLAGVFGTLAAIVFLHSSLMQVVDVIAVGNACTA
                     FAVACALVWQALAGARVAAPR"
     gene            719..1585
                     /gene="rfbD"
                     /locus_tag="VFU90_11255"
     CDS             719..1585
                     /gene="rfbD"
                     /locus_tag="VFU90_11255"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU5480405.1"
                     /translation="MNRRALLIGGSGRLGTAIRATWSDYAIDAPPRAELDLESADGVA
                     NAIDRFSPGVVVNCAAFADVDACERFVERAFAVNAVAVERAARACGDRGVVFVTISTD
                     YVFDGRTQRPYAEDDPAHPLSVYGTSKLAGELLVERLGGRALVIRTCGLYGQSRRAGA
                     VPSFAERILAQARAGEPLAVVSDTIASPTYAEHLAEAIRALLAADASGLFHAAGAGPA
                     SWYDFAAELLAQAGIDAAIEPIAAHEWKAGARRPRFSALDDRKLRAAGFAMPSWKDGV
                     AAYVRRLPGFCA"
     gene            1618..>2668
                     /locus_tag="VFU90_11260"
     CDS             1618..>2668
                     /locus_tag="VFU90_11260"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEU5480406.1"
                     /translation="MELRIDRASVDRCRALAAAIVAPVEAFIAAHSTVSVERAVLRLL
                     GVDGVGPDEIPVPNLIVDALPAAERANGVAPAFGRGLAETGLAPGPLGEAIAAGRFDL
                     GAFAAVPEAAARGALRPHVERALARIRARRDERAAMLERLPQLPPPLLYVIVASGNIY
                     EDRTAAVAAAEAGAQIIAVIRSTGQSLLDFVPYGATTEGFGGTYATQANFGIMRAALD
                     EASERLGRYVMLTNYASGLCMPEIAAMGALERLDMLLNDSMYGILFRDINMKRTFVDQ
                     HFSRMLNGFSGIVINTGEDNYLTTADAVEQAPAVLASQFINEEFAHRAGLPDAQIGLG
                     DAYEIDPDLEDGFLYQ"
BASE COUNT          377 a          919 c          902 g          470 t
ORIGIN      
        1 tcgggttcct gtgcggcgcc gccggcggcg ccctcgcaat cgcgatgacc ctgttgacgc
       61 gttatcgcgc cgtggccgac tgcacgatcc gctacgattg gcgccggatc gcggtcagtg
      121 ggttgggatc ggcggcgatc acgctggccg gtgcgttgat cggctcggcc gacgtcatct
      181 tgatcaaaca cttcttcgac gcggatcaag cggggatcta ttcggcggct tcgctcggcg
      241 gcaaagtgct gctctacttc gtcggattcg ttcccacgat tctgctcccg caggccaccg
      301 agcgccacgt ccgcggcgag cgcacgcgtt acgcgctggc gacgtgcgtg ggcgtgcttt
      361 ttggaatttc gatcgtcggt ctgatcgcgc tcaaactctt cggaatcgtc ctgctgcacg
      421 cgctggtggg gcacgccttc gacgccgccg cgccgctgct gcttccgtac agcgtcgcga
      481 tgatgttctt ggcgctcagc agcgtgctcg gggcgtacgg catcgcaacc caccggatcg
      541 cgttcgccgc tccgctgctc gccggcgtct tcggcacgct ggccgcgatc gtgttcctgc
      601 attcgtcgct gatgcaggtg gtcgacgtca tcgcggtggg caacgcctgt acggcgttcg
      661 ccgtcgcctg cgcgctcgtc tggcaggcgc tggccggcgc gcgcgtcgcc gcaccgcggt
      721 gaatcgccgc gcccttttaa tcggcggaag cggacggctg ggaacggcga tccgcgcgac
      781 gtggtccgac tacgcgatcg acgctccgcc acgtgccgaa ctcgatctcg aaagcgcgga
      841 cggggtggcg aacgcgatcg atcgtttttc gccgggcgtc gtggtcaact gcgccgcttt
      901 tgcggacgtc gacgcctgcg agcgattcgt cgagcgtgcg tttgcggtca acgccgtcgc
      961 ggtcgagcgt gccgctcgcg cgtgcggcga ccgcggcgtc gtcttcgtca cgatcagtac
     1021 cgactacgtg ttcgacggac gcacgcagcg cccgtacgcg gaagacgatc cggcgcaccc
     1081 gctgtcggtc tacggcacct cgaagctggc cggcgaactg ctcgtcgaac ggctcggcgg
     1141 gcgcgcgctg gtgatacgca cgtgcggcct gtacggccaa tcgcggcgcg cgggcgcggt
     1201 gccgtcgttt gcggaacgca tcctcgcaca ggcgcgcgcc ggcgaacctc ttgcggtggt
     1261 gagcgacacg attgcgtcgc cgacgtacgc cgaacatctg gccgaagcga tccgggcgct
     1321 gttggcggcg gatgcttcgg ggctgtttca cgccgccggc gccggcccgg cgagctggta
     1381 cgattttgcc gccgagttgc tggcccaagc cggcatcgac gccgcgatcg aaccgatcgc
     1441 cgcgcacgaa tggaaggccg gcgcccggcg cccgcgcttc tcggcactcg acgaccgcaa
     1501 gctgcgcgcc gccggctttg cgatgccgtc gtggaaagac ggcgttgcgg cgtacgtgcg
     1561 ccggctcccc ggcttttgcg cttgagcccc gcggcgccga gcccgtaaga tagcgctatg
     1621 gaacttcgga tcgaccgggc gtcggtagac cgttgccggg cgctggccgc agcaatcgtc
     1681 gcgccggtcg aggcgttcat cgccgcccat tcgaccgtct cggtcgagcg ggccgtgctg
     1741 cggctcctgg gcgtcgacgg ggttggtccc gacgagattc cggttccgaa cctgatcgtc
     1801 gacgcgttgc cggccgccga gcgcgcgaac ggcgtggcgc cggctttcgg ccgcggcctg
     1861 gccgaaaccg ggctcgcccc cgggcccttg ggtgaagcga ttgccgccgg ccggttcgat
     1921 ttgggcgcct ttgccgcggt tcccgaagcg gccgcacgcg gcgccctgcg tccgcacgtc
     1981 gagcgggcgc tggcgcggat tcgcgcccgc cgcgacgagc gcgccgcgat gctcgaacgg
     2041 ctgccgcagc tgccgccccc gctgctctac gtgatcgtcg ccagcggaaa catctacgaa
     2101 gaccgtaccg ccgcggtcgc cgcagccgaa gccggcgcgc agatcatcgc cgtcattcgt
     2161 tcgaccgggc aatcgctgct cgacttcgtt ccgtacggtg cgacgacgga aggattcggc
     2221 ggcacctacg caacccaggc gaacttcgga atcatgcgcg cggccctcga cgaagcctcg
     2281 gaacggctcg gccggtacgt catgctcacg aactacgcga gcggcttatg catgcccgaa
     2341 attgccgcaa tgggcgcgct cgagcgcctc gacatgctgc tcaacgactc gatgtacgga
     2401 attttgttcc gcgacatcaa catgaaacga acgttcgtcg accagcactt cagccgcatg
     2461 ctcaacggct tttcgggaat cgtcatcaac accggcgaag acaactatct cacgaccgcc
     2521 gacgcggtcg aacaggcgcc ggcggtgctc gcttcgcagt tcatcaacga ggagttcgcg
     2581 caccgcgccg gactgcccga cgcgcaaatc ggcttgggcg acgcttacga aatcgatccc
     2641 gatctagagg acggcttcct ctatcaag
//
LOCUS       DASUCJ010000944         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_209034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000944 DASUCJ010000000
VERSION     DASUCJ010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFU90_11265"
     CDS             <1..590
                     /locus_tag="VFU90_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU5480407.1"
                     /translation="IDPATDEILHHSALQNDVVMEIVAAASTGGMHLQLYRNDEYYAE
                     ARNRFSDLYASLAKTEPVVVPSLRETFAFSGATKAVVVADPPDAEHYANQLRERLAGR
                     AYVTRSLPEFVEVLDPTVDKGDALGFVAARLGIPLRAVMAIGDSWNDAPLLRAAGFAV
                     AMGSAPPELRELADAIVADVAHDGVAEALERYVPG"
     gene            587..>1070
                     /locus_tag="VFU90_11270"
     CDS             587..>1070
                     /locus_tag="VFU90_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5480408.1"
                     /translation="MNRQRLSARRRKPSAGSRMRPFWLPIAAGVLLVAGITVFVWTWS
                     GFEPSRVEVTGNRVVPAAEIAARAGVLRNVNMWLQNTGAMANRVATIPYIAKARVYRI
                     PPSSIVVAVRERTPFAVVATGTQEAVVDRDLRILEPSAGGGTLPRLVVAEPDERMRPG
                     R"
BASE COUNT          169 a          334 c          382 g          185 t
ORIGIN      
        1 tcatcgaccc cgctactgac gagattctcc atcacagcgc gctgcagaac gacgtggtga
       61 tggaaatcgt cgcggcggcc tcaacaggcg gaatgcacct gcagctgtat cgcaacgacg
      121 aatattatgc cgaggctcgc aaccggtttt cggacctcta cgcatcgctg gcgaagaccg
      181 agccggtcgt ggtgccgtcg ctgcgcgaga cgtttgcgtt cagcggcgcc accaaggccg
      241 tcgtggtcgc cgacccgccc gatgcggagc actatgcgaa ccagctgcgc gaacggctcg
      301 ccgggcgcgc gtacgtaacg cgcagcttgc cggaattcgt cgaagtgctc gacccgacgg
      361 tcgacaaggg cgacgcgctg ggtttcgtgg cagcccgcct ggggatacct ttgcgggccg
      421 tgatggcgat cggagactcg tggaacgatg cgccgttgct gcgcgctgcc ggctttgcgg
      481 tggcgatggg gtcggcgccg cccgaactgc gcgaactggc cgacgcgatc gttgccgacg
      541 tagcgcacga cggggtcgcc gaagcgctcg aacggtacgt gcccggatga accggcagcg
      601 cctctccgcc cggcggcgca agccgtcggc cggctcgcgg atgcggccgt tttggcttcc
      661 gatcgcagcc ggagtgctgc tggttgccgg catcaccgtc ttcgtatgga cgtggtcggg
      721 gttcgagccg tcgcgcgtcg aggttaccgg caaccgggtg gttccggccg cggagatcgc
      781 ggcgcgcgcg ggcgtgctcc gcaacgtcaa tatgtggctg caaaacaccg gagcgatggc
      841 caaccgggtt gcgacgatac cgtatatcgc caaagcccgc gtctatcgaa ttccgccgtc
      901 ctcgatcgtg gtcgcggttc gcgaacgcac gccgtttgcg gtggttgcga ccggtacgca
      961 ggaggccgta gtcgatcggg atttgcgaat cctggaaccg tcggcgggtg gtggaacgct
     1021 gccgcgtctg gtcgtggcgg agccggacga acgtatgaga ccggggcgat
//
LOCUS       DASUCJ010000945         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_942493, whole genome shotgun sequence.
ACCESSION   DASUCJ010000945 DASUCJ010000000
VERSION     DASUCJ010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942493"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VFU90_11275"
     CDS             complement(<1..1525)
                     /locus_tag="VFU90_11275"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480409.1"
                     /translation="MRTSTLRVIPLVLAAAVLPVSAHSQSSDLYSDMHWREIGPTRAG
                     RARAVSGSAREPNVAYIGFDNGGVWRSTDYGSTWTPLFDNESTGSIGAIAVAPSNPNI
                     IYVGTGAGIIRPDLSTGNGMYKSTDAGKTWTRIGLDDSQMIAAVDVDPFNPDRLFVAV
                     LGHPYGPNEQRGVFRSTDGGHTFTKVLYKDDYTSANDVHIDPSNPNVVYASLWQQQEA
                     FHEGGGFGGSGGGLFKSTDGGTTWTELKDGLPSSVLEANIAVSRSNPNIVYAMVAQPS
                     NRPGAQAAGYGAGVVGFYKSTDAGAHWTLVDGPGLTSRPVDPRPLVRIGGGDLPTIIV
                     DPQNENVVYSSSTVLWRTDDGGITWSAVRGAPGGDDYQKTWINPLNPNIIVQVSDQGG
                     VVSANRGVSWSNWYTQPTAAMYHVSTDNTFVYRVCGGQQDSGSACVKSRSDDGEITFN
                     DWHPVNIQEYGIAAPDPRDPDLVYGSMRTNVSLYNRKTGQTTNVGPSAEQRGAAYGRN
                     "
BASE COUNT          256 a          521 c          576 g          299 t
ORIGIN      
        1 cgttgcgacc gtacgcagcg ccgcgctgtt ctgcgctggg tccgacgttc gtcgtctgcc
       61 ccgtcttgcg attgtagagc gacacgttgg tccgcatgct gccgtacacg aggtcggggt
      121 cgcgcggatc cggcgcggcg ataccgtact cctggatgtt caccgggtgc cagtcgttga
      181 acgtgatctc gccgtcgtcg gaccggctct tcacgcacgc ggatccggag tcctgctggc
      241 cgccgcacac gcggtagacg aacgtgttgt cggtggacac gtgatacatc gccgccgtgg
      301 gctgcgtgta ccagttgctc caggacacgc cgcgattggc cgagacgacg ccgccctggt
      361 cgctcacctg cacgatgatg ttgggattga gcgggttgat ccacgttttc tggtagtcgt
      421 cgccgcccgg cgcgccgcgc acggcagacc aggtgatgcc gccatcgtcg gtgcgccaga
      481 gcacggtcga cgagctgtac accacgttct cgttctgcgg atcgacgatg atcgtcggca
      541 gatcgccgcc gccgatgcga acgaggggac gcggatcgac cggccggctc gttaggcccg
      601 gaccatcgac gagcgtccag tgcgcgccgg catcggtgga cttgtagaat ccgaccacgc
      661 ccgcgccgta gccggcggct tgggcgcccg ggcgattgga cggttgcgcc accatcgcgt
      721 acacgatgtt ggggttgctg cgcgagacgg cgatgttcgc ctcgagcacc gacgacggca
      781 ggccatcctt gagctccgtc cacgtcgtgc cgccgtccgt cgacttgaac aggccgccgc
      841 ccgagccgcc gaatccaccg ccctcgtgaa acgcctcctg ctgctgccac agcgatgcgt
      901 acacgacgtt cgggttggac ggatcgatgt gcacgtcgtt ggcgctcgtg tagtcgtcct
      961 tgtacaacac tttcgtgaac gtgtggccgc cgtccgtcga gcggaacacg ccgcgttgtt
     1021 cgttaggccc gtacggatgg ccgaggaccg cgacgaacag gcggtccgga ttgaacggat
     1081 cgacgtcgac cgcggcgatc atctggctgt catcgagccc gatgcgcgtc cacgtcttgc
     1141 cggcgtcggt ggacttgtac atgccgttgc ccgtggacag gtcgggccga atgatgcccg
     1201 cgccggtgcc cacgtaaatg atgttcgggt tggatggcgc gacggcgatg gcgccgatgg
     1261 atcccgtgga ttcgttgtcg aagagcggcg tccaggtgga cccgtagtcg gtcgagcgcc
     1321 acacgccgcc gttgtcgaat ccgatgtacg cgacgttggg ctcacgcgcc gagcccgaga
     1381 ccgcgcgtgc gcggccggcc cgcgttgggc cgatttcgcg ccagtgcatg tccgagtaca
     1441 aatcgctgga ttgcgagtgc gccgacaccg gaagcacggc ggcagcgagg acgagtggga
     1501 tgacgcgaag tgtggacgtg cgcacgaaac cggcctcgac gaattgagcg aaagggtgac
     1561 gacactaggt cggtgaccgc gaggaatatg gggctgggac ctgcgcgcgg ctatgcgcgc
     1621 ggcgattgcg ccagatgtcg ggtcgttcta tc
//
LOCUS       DASUCJ010000946         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_911846, whole genome shotgun sequence.
ACCESSION   DASUCJ010000946 DASUCJ010000000
VERSION     DASUCJ010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911846"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFU90_11280"
     CDS             <1..787
                     /locus_tag="VFU90_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5480410.1"
                     /translation="KASPYLVNAVGSSNWRVNVTFASLSAVAGGLIVLLLHDGPYALP
                     NQPFDFSQVTAVYRNRGVRLANFGYFGHMWELYAMWTWMAAFVTASETARFAGLESSA
                     DVRTRAALVTFAVVGSGAVGCWLGGKYADRFGRTTITSLAMAASALCCLVAGWFFGQP
                     LTALVPLLIVWGVSVVADSAQFSTAVSELAPKEYVGTALTLQTSLGFLLTTVTISLLP
                     LVAGRIGWRWSMTALAPGPILGVVAMQALRRRPEAIRLANGKR"
     gene            complement(794..>1147)
                     /locus_tag="VFU90_11285"
     CDS             complement(794..>1147)
                     /locus_tag="VFU90_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEU5480411.1"
                     /translation="LPGRRPAPLPAVPRPALHHSAPDAEDPPAVSAALEDNGAFVIHA
                     AARTVEIEGDFTDWQPLLLTEREPAAWTLPIPLAAGTYRFSIRIDGGAWFAPAGVTKV
                     PDEFGPGTVGLLTVP"
BASE COUNT          157 a          374 c          410 g          206 t
ORIGIN      
        1 caaagcgtcg ccgtatctcg tcaacgccgt cggctcatcc aactggcgcg tgaacgtgac
       61 gttcgcgtcg ctgtcggccg tcgccggcgg attgatcgtg ctccttctcc acgacggtcc
      121 gtacgcgctg ccgaaccagc cgttcgattt cagtcaggtc accgctgttt atcgcaatcg
      181 cggcgtccgg ctcgcgaact tcggctactt cggccacatg tgggagctgt acgcgatgtg
      241 gacgtggatg gcggcgtttg tgaccgcgag cgagacggca cggttcgccg gcctcgagag
      301 cagcgccgat gtccggacgc gcgccgcgct cgtcacgttc gccgtcgtcg gcagtggggc
      361 ggtgggttgc tggcttgggg ggaaatacgc cgatcgcttc gggcgcacca cgatcaccag
      421 cctcgcgatg gcggcgtcgg cgctgtgctg tctcgtcgcg gggtggttct tcggccagcc
      481 gctcaccgcg ctcgtcccgt tgctcatcgt gtggggggtc tcggtcgtcg ctgattcggc
      541 gcagttctcg accgccgtca gcgagctggc gccgaaggag tacgtcggca ccgccctgac
      601 gctgcagacg agcctcggct tcctcctcac gacggtgacg atttccctgc tgccgctggt
      661 cgcgggcaga atcggctggc gctggagcat gacggcgctc gctccgggac cgatcctcgg
      721 cgtggtagcg atgcaggcgt tgcgacggcg accggaggcg attcgattgg cgaatgggaa
      781 acgctgagga tcgctacggg accgtcagca accccaccgt gccgggtccg aactcgtccg
      841 gcactttggt gaccccggcc ggggcgaacc aggcaccacc gtcgatgcgg atgctgaagc
      901 ggtaggtccc ggccgcgagc ggaatcggca gcgtccacgc tgccggctcg cgctcggtca
      961 gcagcagcgg ctgccaatca gtaaagtctc cttcgatctc gacggtgcgg gctgcggcgt
     1021 gaatcacgaa tgcgccgttg tcttcgagcg ccgcgctcac cgcgggggga tcttcggcgt
     1081 ccggcgctga atgatgcagc gccggccgtg ggacggcagg aagcggtgcc gggcggcggc
     1141 ccggcag
//
LOCUS       DASUCJ010000947         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_621223, whole genome shotgun sequence.
ACCESSION   DASUCJ010000947 DASUCJ010000000
VERSION     DASUCJ010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621223"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1199)
                     /locus_tag="VFU90_11290"
     CDS             complement(33..1199)
                     /locus_tag="VFU90_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5480412.1"
                     /translation="MTMDLDAARTPLTVLAEDETMFRDAVAAFADEQVRPRVQAMDRA
                     GKLDPDLIPGFFELGLMGIGVPEAYGGAGGTMMMVTLAVEAVSRVDASSAILMDVQNT
                     LVNSPLTRFGTDAQRKQYLTKLTSGTVGAYALSEPGSGSDAFGLATRAERRGDAWVLT
                     GRKLWITNGAEAEIYIVFATVDPSAGYRGITAFIIERGFAGFAVGKKEDKLGIRASST
                     TELILDGCVVPAANVLGPVGQGYKIAIETLNEGRIGIGAQMIGVARGALDATLQYVRE
                     RTQFGKPLAEFQGIQFQLAQAATELEAARLMVYNAALLEDAGRPFTQEAAMAKLFSSQ
                     VTERVTSLCVELHGGYGYTKDYPVEKFYRDAKIGTIYEGTSNMQLQTIAKMLLK"
     gene            complement(1252..1728)
                     /locus_tag="VFU90_11295"
     CDS             complement(1252..1728)
                     /locus_tag="VFU90_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEU5480413.1"
                     /translation="MKAILLVDHGSVKDAANRMIDCVGHLVQAIAGDGVIVRVSHMEL
                     AEPTIAQAFAQCVEAGATEVVVFPYMLSPGKHSTRDIPRMARQAAAPFPHISVSVTPA
                     FGVHDKLAEVVLERAGVEPRGRRVPAPCMCVRPQGTPERWCGDACREAATNVTVER"
     gene            complement(1771..>2538)
                     /locus_tag="VFU90_11300"
     CDS             complement(1771..>2538)
                     /locus_tag="VFU90_11300"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU5480414.1"
                     /translation="DAVNVPDGPRAQSRMGALLTSVLIEQQVGIETVTHYACRDRNLL
                     GMLSDLLGASAMGLRNLLIITGDPPKMGPYPDATAVFDIDSIGLTNLVSRLNRGLDPG
                     GHAIGAPTQFAIGVGVNPAAIDPAYELRRFEWKVDAGAEFAITQPVFDVDQLHRFLAS
                     IDHVRIPVIAGIWPLVSARNAEFLANEVPGVVVPDAVIHRMRRASEQSKEHGLAEGIA
                     LAREMLEQVRGTVQGVQVSAPFGKVELALEVFDSATR"
BASE COUNT          431 a          894 c          775 g          438 t
ORIGIN      
        1 cagcacacga accgcaacgg ggcttcccgt cgctacttga gcagcatttt cgcgatcgtc
       61 tgcagctgca tgttgctggt gccctcgtag atcgtcccga tcttcgcgtc gcggtagaac
      121 ttttcgaccg ggtaatcctt cgtgtaccca tacccgccgt gcagctccac gcacagcgac
      181 gtcacccgtt cggtcacctg cgacgagaac agcttcgcca tcgcggcttc ctgcgtgaac
      241 ggccggcccg catcctcgag aagcgccgcg ttgtacacca tgagccgagc ggcctcgagc
      301 tccgtcgccg cctgcgccaa ctgaaactgg atgccctgga attcggccag cggtttgccg
      361 aactgcgtcc gctcgcgcac gtactgcagg gtcgcgtcga gcgcgccgcg cgcgacgccg
      421 atcatctgcg cgccgatgcc gatgcgccct tcgttcaagg tctcgatcgc gatcttgtag
      481 ccttgcccga ccggccccag cacgttcgcc gccgggacca cgcacccatc gaggatcagt
      541 tcggtcgtgc tcgacgcgcg aatgcctaac ttgtcctctt tcttgcccac cgcgaaacct
      601 gcgaacccgc gctcgatgat gaacgcggtg atcccgcgat atccggccga cgggtcgacc
      661 gtcgcgaaca cgatgtagat ctcggcctcg gcgccgttcg tgatccagag cttgcggcca
      721 gtgagcaccc aggcgtcacc gcggcgctcg gcgcgcgtcg ccagcccgaa agcatctgag
      781 cccgaacccg gctccgacaa cgcgtacgcc ccaaccgtgc ccgacgtgag cttcgtcaag
      841 tattgcttgc gttgggcatc ggtgccgaac cgcgtgagcg gcgagttcac gagcgtgttc
      901 tggacgtcca tcagaatcgc cgacgatgca tccacccggc tcacggcctc gaccgcgagc
      961 gtcaccatca tcatggttcc gccggctccg ccgtatgcct ccggcacgcc gatacccatg
     1021 agcccgagct cgaagaatcc gggaatcaaa tccggatcga gcttgccggc acgatccatt
     1081 gcctgcacgc gcggacgcac ttgctcgtcg gcgaacgccg cgacggcatc gcggaacatc
     1141 gtttcatctt cggccagcac ggtgagtggc gtgcgggccg cgtcgaggtc cattgtcatg
     1201 cgatccaatg tatcgggtgg ttcccgcttg ggtgagcggc tgagatcggc gctagcgctc
     1261 gaccgttacg ttcgtcgccg cttcgcggca ggcatcgccg caccagcgct cgggagtacc
     1321 ctgcggccgc acgcacatgc acggcgccgg cacgcgacgt ccccgcggtt cgacgcccgc
     1381 gcgctccaac accacctccg ccagcttgtc gtgcacgccg aatgccggcg tcaccgacac
     1441 actgatgtgc ggaaacggcg ccgcggcctg gcgcgccatg cgcggaatgt cgcgcgtcga
     1501 gtgcttcccc ggcgacagca tgtacgggaa gaccactacc tccgtcgcgc ccgcctcgac
     1561 gcactgcgcg aacgcctgcg cgatggtcgg ctccgccaat tccatgtgcg acacgcgcac
     1621 gatcacaccg tcaccggcga tcgcctgcac gagatgccca acgcagtcga tcatgcgatt
     1681 ggcggcatct ttgaccgagc cgtgatccac gagcaagatg gctttcatgg gcggggctcc
     1741 atgtcgtgac cgatgcgtcg actctctaag ctaacgcgtg gcggagtcga agacctcgag
     1801 agccagctcc actttaccga acggcgccga gacctgcacg ccctgcacgg tgccgcgcac
     1861 ctgctcgagc atttcgcgcg cgagcgcgat tccttcggcc agcccgtgct ccttggattg
     1921 ttcgctcgcg cgacgcatcc ggtgaatcac cgcatccggc acgaccacgc caggcacttc
     1981 gttggcgaga aactcagcgt ttcgcgccga cacgagcggc cagattccgg cgatcacggg
     2041 aatgcgcaca tgatcgatcg acgcgaggaa gcggtgcagt tgatccacgt cgaagacggg
     2101 ctgggtgatc gcgaactccg cacccgcatc caccttccat tcgaagcggc gcagctcgta
     2161 cgccggatcg atggcggcgg gattcactcc gacgccaatg gcgaactgtg tcggcgcgcc
     2221 gatcgcgtgg ccgccgggat cgaggccccg attgaggcgg ctcaccagat tcgtgagacc
     2281 gatcgagtcg atgtcgaaga ccgcggtggc gtcggggtag gggcccatct tcggcggatc
     2341 gccggtgatg atcagcagat ttcgcagccc catggccgac gcgcctaaca aatcgctcag
     2401 catgccgagc aggttgcggt cgcggcaggc atagtgcgtc accgtctcga tcccgacctg
     2461 ttgctcgatg agcacgctcg tcagcaacgc gcccatgcga ctctgcgcac gcggcccgtc
     2521 gggaacgttg acggcatc
//
LOCUS       DASUCJ010000948         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_911857, whole genome shotgun sequence.
ACCESSION   DASUCJ010000948 DASUCJ010000000
VERSION     DASUCJ010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911857"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1094)
                     /locus_tag="VFU90_11305"
     CDS             complement(<1..>1094)
                     /locus_tag="VFU90_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5480415.1"
                     /translation="GWVDDTGEARAGLELALDSLLRGVDGHAVIARDAHGRRFDSPAD
                     SDVAPESGDAVQLTINQELQEISVRALSDAIDKMGATGGDIVILDPEDGEIRAMASDR
                     VGQPSFGNPAISEPFEPGSTVKPLFAAELLQRGLAHPSDTVNTENGVYTIEGRTIHDE
                     EPKPRLTLAQAIEYSSNIAMVKFVSRLTPRQEFETLRDYGFGMPTGVPVPGEASGILY
                     PPSDWSKQSPASMAIGYEVAVTPLQLAAAYGAIANGGDLLEPALIKEIRSRDGTILYR
                     HDRRVVRHLFSPQVAATVRKMMIGVVQGGTSTEAQLARFTLAGKSGTARRGATKHGYT
                     AGHYTASFIGLFPADKPLYVIIVKLDDPKS"
BASE COUNT          170 a          386 c          345 g          193 t
ORIGIN      
        1 tcgacttggg atcgtcgagc ttcacgatga tcacgtacag gggcttgtcc gccgggaaga
       61 ggccgatgaa cgacgcggtg tagtggccgg cggtatagcc gtgcttggtc gcgccgcggc
      121 gcgccgtgcc gctcttgccg gcaagcgtga aacgcgccag ctgcgcttcg gtgctcgtgc
      181 cgccctgcac cacgccgatc atcattttgc gcaccgtcgc cgcaacctgc ggcgagaaca
      241 gatgcctaac gactcggcga tcgtgacggt agaggatggt cccgtcgcgc gagcggatct
      301 ccttgatcaa cgccggctcc aacagatcgc ctccgttggc gatcgcaccg tacgccgcgg
      361 ccaattgcag cggcgtcacc gccacctcgt agccgatcgc catcgacgcc ggcgactgct
      421 tcgaccagtc cgacggcggg tacaaaatcc ccgatgcttc gccgggaacc ggcacgcccg
      481 tgggcatgcc gaagccgtag tcgcgcagcg tctcgaactc ctgccgtggc gttaggcggg
      541 agacgaactt caccatggcg atgttgctcg agtactcgat cgcctgcgcg agtgtgagtc
      601 gcggcttcgg ctcctcgtcg tggatcgtgc ggccttcgat cgtgtagacg ccgttctcgg
      661 tgttcaccgt gtcgctcgga tgcgccagac cgcgctgcag cagctccgcc gcgaacaatg
      721 gtttcacggt cgatcccggc tcgaacggct cactgatggc gggattcccg aagctcggct
      781 gcccaacgcg gtcgctcgcc atcgcgcgga tctcgccgtc ctccggatcg agaatcacga
      841 tgtcgccacc cgtcgcgccc attttgtcga tcgcatccga cagcgcgcgc accgagatct
      901 cctgcagctc ctgattgatc gtgagctgca ccgcgtcgcc cgactccggt gcgacgtcgc
      961 tgtcggccgg cgaatcaaac cgccggccgt gcgcgtcgcg cgcgatcacc gcgtgtccat
     1021 cgacgcctcg caagagcgag tcgagcgcca actcgagacc ggcgcgcgcc tcgccggtgt
     1081 catccaccca gccg
//
LOCUS       DASUCJ010000949         1455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1156013, whole genome shotgun sequence.
ACCESSION   DASUCJ010000949 DASUCJ010000000
VERSION     DASUCJ010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156013"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..>1455
                     /locus_tag="VFU90_11310"
     CDS             11..>1455
                     /locus_tag="VFU90_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480416.1"
                     /translation="MPPSGGQVRVNGEDLYLIYDALRGSIGYVPQDDIVHPELTVFEA
                     VKYSARFRLPPDYSEEEINRRVDETLRDLGLEGVRNLQIGRPEKKVLSGGQRKRVNIA
                     LELVTDPVILFLDEPTSGLAADDTTALIHLLSELAKTTGKTIVMTIHQPAKDEFEKFN
                     LCFIMGYGGVPTYFGPTGESAYSFFGSIADRTSPGHKRVDNPRDMFDMLNVRERLTLE
                     DMRRGNPQVVRTAARLEAAKAWRAEFFRNDNPVFQRMFSGKRAVGAAPGARGVPHKGG
                     VAKLRQFGLLVSRYWKVKLRDHMGSAIMFLQAPVIGLLLWVVFGAQKESVPYWCLGAL
                     QELSSKNNMSQSTTTNLLNSMRPTTDHTAAMFFVVVSCIWFGTSNAAREIVTERAIYL
                     RERMVNLGLVNYVLSKYVLLTFFCVVQCAVLLAIVFFSLGFSGGPMAFLMELGNLIAL
                     GMNATALGLMISSLVASAEAAMALTPIALIP"
BASE COUNT          253 a          482 c          459 g          261 t
ORIGIN      
        1 caatgggtac atgccgccga gcggcggcca ggtccgcgtc aatggcgagg acctctattt
       61 gatttacgac gccctgcgcg gctcgatcgg gtacgtcccg caggacgaca tcgtgcaccc
      121 cgagctcacc gtcttcgaag cggtgaagta ctccgcgcgg ttccgccttc cgccggacta
      181 ttccgaagaa gaaatcaacc gccgcgtcga cgagacgctc cgcgacctcg ggctcgaagg
      241 cgtgcgcaat ctgcagatcg gccggccgga aaagaaagtc ctctcgggcg ggcagcgaaa
      301 gcgcgtgaac atcgccctcg agctcgtgac ggacccggtg attctctttc tcgacgagcc
      361 gacgagcggc ctcgccgccg acgacacgac ggcgctcatt catcttctat ccgagctcgc
      421 caagacgacc ggcaagacga tcgtcatgac gatccaccag ccggcgaaag acgaattcga
      481 gaagttcaat ctgtgcttca tcatggggta cggcggcgtg ccgacgtatt tcggcccgac
      541 gggggagtcg gcgtacagct tcttcggatc gatcgccgat cgcacgtcgc ccggccacaa
      601 gcgcgtcgac aacccgcgcg acatgttcga catgctcaac gtgcgcgagc ggctgacgct
      661 cgaggacatg cgccgcggca atccgcaggt cgtccgcacg gcggcgcgcc tcgaagcggc
      721 caaagcgtgg cgggcggagt tcttccgcaa cgacaatccc gtcttccagc ggatgttctc
      781 cggcaagcgc gccgtcggcg ccgcgcccgg agcccgcggc gtgccccaca agggcggcgt
      841 ggcgaagctg cgccaattcg ggctcctcgt gtcgcgctat tggaaagtga agctgcgcga
      901 ccacatgggg tccgccatca tgttcctcca ggcgcccgtc atcgggctcc tcttgtgggt
      961 cgtcttcggc gcgcagaaag aatcggtgcc gtattggtgc ctcggggcgc tgcaagagct
     1021 ctcgtcgaag aacaacatga gccagagcac gaccacgaat ctgctcaact cgatgcggcc
     1081 gacgacggac cacaccgccg cgatgttctt cgtcgtcgtg agctgcatct ggttcggcac
     1141 gagcaacgcg gcgcgcgaaa tcgtgacgga gcgcgcgatt tacctgcggg agcgcatggt
     1201 gaacctcggg ctcgtgaact acgtgctttc gaaatacgtt ttgctgacgt tcttttgcgt
     1261 cgtgcagtgc gccgtgctcc tcgcgatcgt gttcttctcg ctcgggttca gcggcggccc
     1321 catggcgttc ttgatggagc tcggcaatct catcgccctc gggatgaacg cgacggcgct
     1381 cggcctcatg atctcgtcgc tcgtcgcgtc ggccgaggcc gcgatggcgc tcacgccgat
     1441 cgcgctcatc ccgca
//
LOCUS       DASUCJ010000950         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_789453, whole genome shotgun sequence.
ACCESSION   DASUCJ010000950 DASUCJ010000000
VERSION     DASUCJ010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789453"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1481
                     /locus_tag="VFU90_11315"
     CDS             28..>1481
                     /locus_tag="VFU90_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480417.1"
                     /translation="MSAQRAYDPFAPQRMLPELDRVRRLARAAAGSHDRDAEASLAEL
                     TARVNQAIRLAAGIGTEAVVDHETWAAEEPIHASVTVYDRVDEPMDVSVSFPPADARA
                     QPARAIAPLTSAQFSDTMRDTVITQPWWLARPRVGDIFGVPIVAKPEDELHGLTARVS
                     GEIGGVAIDDTVPVVYRYADPVKGEIQHPIAIVPAVSRTVDQPVQYVPANRPFDRVVR
                     VELRSASNHARTVRVSLELPRGLTADSLSRTIGLPDYAGNFGGEGEPQGMPGGRPVAG
                     NSPMRAVEFHVRGTLPAGRHEISAIAESEGKRYTEGYTLVEYDHIRPQRLFRDARLEL
                     SAVDVQVPKTLNVAYVPGVGDNVAPMLAQLGIPVTVIDPEKLADADLSRYTTVVVGPR
                     AYESSTALVAANPKLFDFARAGGTLVVQYGQFEMANQGMMPYPITIQRPGDRVTEEDA
                     PVRVIDPSSPILATPNRIDTADWQGWVQERSLYMP"
BASE COUNT          243 a          508 c          494 g          236 t
ORIGIN      
        1 tcgatcgact ccctccccgg tgcaatcgtg agcgcgcagc gcgcgtacga tccattcgcc
       61 ccccagcgaa tgctgccgga gctcgaccgc gtcaggcggc tggcgcgcgc cgcggctggc
      121 agccacgatc gcgatgccga ggcctcgctc gccgagttga ccgcccgggt gaatcaggca
      181 attcgtctcg cggccgggat cggcaccgag gccgttgtcg atcacgagac gtgggccgcc
      241 gaggaaccca tccacgccag cgtgacggtg tacgatcgtg tcgacgagcc gatggacgtg
      301 agcgtcagct tcccaccggc cgatgcgagg gcacagcctg ctcgcgccat cgctccgttg
      361 acgagcgccc agttcagtga cacgatgcgc gacaccgtca tcacgcagcc gtggtggctc
      421 gcgcgtcccc gtgtcggcga cattttcggc gtgccgatcg tcgccaagcc ggaggacgag
      481 ctgcacggcc tcacggcgcg tgtgagcggc gagatcggcg gcgtcgccat cgacgacacg
      541 gttcccgtcg tctatcgtta tgccgaccca gtgaaggggg agatccagca tccgatcgcg
      601 atcgtccccg cggtgagcag aaccgtcgat cagccggtgc aatacgttcc cgccaatcgg
      661 cccttcgacc gcgtcgtccg cgtggagctg cgctcggcga gcaatcacgc gcgcacggta
      721 cgcgtttccc tcgagctgcc gcgcgggctg acggcggact cgctctcgcg cacgatcggg
      781 ctgcccgatt atgccggcaa cttcggcggt gagggggagc cgcagggtat gcccggcggt
      841 cggcccgttg ccggcaactc gccgatgcgg gcggtggagt ttcacgttcg cggaacgctt
      901 cccgcgggtc gtcacgagat ctcggccatt gccgagagtg aaggaaagcg gtacaccgaa
      961 ggctacacgc tcgtggaata cgaccacatc cggccccagc ggctgttccg cgacgcgcgc
     1021 ctggagctca gcgccgtcga cgtgcaggtt ccaaagacac tcaacgtggc gtacgtgccc
     1081 ggcgtgggcg acaacgtcgc gccgatgctc gcgcagctgg gcatccccgt gacggtcatc
     1141 gaccccgaga agctggccga cgcggatctc tcccgctaca ccacggtcgt cgtcgggccg
     1201 cgtgcatacg agtcgagtac cgcgctcgtt gcggcgaatc caaagctgtt cgacttcgcc
     1261 cgggccggcg gcaccctcgt cgtgcaatac gggcagttcg agatggcgaa tcaggggatg
     1321 atgccgtacc cgatcacgat ccaaaggccc ggcgatcgcg taacggagga ggatgccccg
     1381 gtccgcgtga tcgacccatc ctcgccgatt ctcgccacgc caaatcgcat cgacacggcg
     1441 gattggcagg gatgggttca ggagcgttcc ctgtacatgc c
//
LOCUS       DASUCJ010000951         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_942538, whole genome shotgun sequence.
ACCESSION   DASUCJ010000951 DASUCJ010000000
VERSION     DASUCJ010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942538"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2042
                     /locus_tag="VFU90_11320"
     CDS             <1..>2042
                     /locus_tag="VFU90_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5480418.1"
                     /translation="DGKWVSYFSDKSGEYQLVIASADGSAPARTIALVHPTHYYTPSW
                     SPDGKHIVFQDTNLRLWVVDVATGKATDIGGDDWMVPERSLNPAWSPDSRWIAYVRRL
                     KSLYRAIFVYDMQTGQTHQITDGLADATWPAWDASGKYLWFFASTDLALGSGWLDMSS
                     YGHTETKALYLAVLSKNDPSPLLPESDEDTGIPQEQTPGMPPLPPRERPRRPARTDTT
                     ADTTAARRIPPDSLKAMLAPRRHVEIDFDGLLQRVVPVSEIAERDYRELRSGVPGMVF
                     FTENLPETGTKEERFSGGTLHRYDLKERKAMPFVQNVADYFVSADGKKLLYRTPGPEG
                     NLFLVDATTHDAPPAGKGKLNAQLRMLVDPTAEFKQIFDEGWRNQRDYIYVKNLQGTD
                     WVKDKAMYGQLLPYVKHRDDLNYLLDMMGSEIAIGHSFVRGGDIPGEMQPPVGLLGAD
                     FAVENGRYRITKIYGTDSWNPDLHAPLTAPGVQVRVGDYLLAVNGQELRAPDNVYRLF
                     DGTANHQTMITVNERPTMDGARRVTVIPVANDAGLRARAWVEHNRHLVDSLSHGELAY
                     VHLPNTAEGGYTSFNRYYFAQQDRKGAIIDERYNGGGSAADYIIDVLQRTFDGYFNNP
                     VGDRYPFTSPAAGIWGPKVMIINEMSGSGGDLMPYMFKYRKVGQLVGERTWGGLVG"
BASE COUNT          383 a          639 c          692 g          328 t
ORIGIN      
        1 cggacggcaa gtgggtgtcg tacttcagcg acaaatcggg cgagtatcag ctggtgatcg
       61 cttctgccga cggcagcgcg cccgcgcgca cgatcgcgct cgtgcatccg acgcactact
      121 acacgcccag ctggtcgccc gacggcaagc acatcgtctt tcaggatacg aatctccggt
      181 tatgggtggt ggacgtcgcg acggggaagg cgaccgacat cggcggcgac gactggatgg
      241 tgccggagcg gtcgctcaac ccggcgtgga gccccgattc gcgctggatc gcgtacgtta
      301 ggcgtctcaa gtcgctctat cgggcgatct tcgtctacga catgcagacc ggccagacgc
      361 atcagatcac cgacggcttg gccgatgcga cgtggccggc gtgggatgcg agcggcaagt
      421 acctctggtt cttcgcgtcc accgatcttg cgttaggctc gggctggttg gacatgtcga
      481 gctacggcca cacggaaacg aaggcgctct atctcgccgt gttgtcgaag aacgatccgt
      541 cgccgttgct gccggagagc gacgaggaca cggggatccc gcaggagcag acgccgggca
      601 tgccgccgct gccgccgcgc gagcggccgc ggcgtccggc gcgcacggac acgacggcgg
      661 acacgaccgc ggccaggcgc attccgcccg attcgctcaa ggccatgctg gcgccgcgcc
      721 ggcacgtcga gatcgatttc gatggattgc tgcagcgcgt ggtgcccgtc tccgaaatcg
      781 ccgagcgcga ctaccgggag ctgaggagcg gcgtgccggg catggtgttc ttcaccgaga
      841 acctgccgga gaccggaacg aaggaagagc ggttcagcgg cggcacgttg caccgctacg
      901 atctcaagga acgcaaggcg atgccgttcg tgcagaacgt cgccgattat ttcgtgagcg
      961 cggatggaaa gaagctgttg taccggacgc cgggtcccga ggggaatctc ttcctcgtcg
     1021 acgcgacgac acacgacgcg ccgccggccg gcaagggcaa gctcaatgcc caactccgca
     1081 tgctcgtcga cccgacggcg gagttcaagc agatcttcga cgaagggtgg cgcaaccagc
     1141 gcgactacat ctacgtcaag aatctgcagg gcacggactg ggtgaaagac aaggcgatgt
     1201 acggccagct gctgccctac gtgaagcacc gcgacgatct caactacctg ttggacatga
     1261 tgggatcgga gatcgccatc gggcactcgt tcgtgcgcgg cggcgacatc cccggcgaga
     1321 tgcagccgcc cgtcggtttg ttaggcgctg acttcgccgt ggagaacggc cgctacagga
     1381 tcacgaagat ctacggcacc gacagctgga atccggatct gcacgcgccg ctcaccgcgc
     1441 ccggcgtgca ggtgcgcgtc ggcgattatc tgttggcggt gaatgggcag gagctgcggg
     1501 cgcccgacaa cgtctatcgg ctgttcgacg ggacggccaa ccaccagacg atgatcacgg
     1561 tcaacgagcg tcccaccatg gatggcgcgc gccgggtgac ggtgattccc gtggcgaacg
     1621 acgcgggtct ccgcgcccga gcgtgggtgg agcacaaccg gcacctcgtc gattcgctgt
     1681 cgcacggcga gttggcctac gtgcacctgc ctaacacagc cgagggcggc tacacgagct
     1741 tcaaccgcta ctatttcgcg cagcaggacc gcaagggcgc gatcatcgac gagcgctaca
     1801 acggcggcgg gtcggcggcc gactacatca tcgatgtgct gcagcgcacc ttcgacgggt
     1861 acttcaacaa tccggtgggc gaccggtatc cgttcacgag tcccgccgcc gggatctggg
     1921 gaccgaaggt gatgatcatc aacgaaatgt ccggatccgg cggcgacctc atgccctaca
     1981 tgttcaagta ccggaaggtg ggccaactgg tgggcgagcg cacgtggggc ggactcgtgg
     2041 gc
//
LOCUS       DASUCJ010000952         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_407065, whole genome shotgun sequence.
ACCESSION   DASUCJ010000952 DASUCJ010000000
VERSION     DASUCJ010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407065"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFU90_11325"
     CDS             complement(<1..754)
                     /locus_tag="VFU90_11325"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5480419.1"
                     /translation="MSDLSGESTLDARSTSPLRLLDRPGREGIAIVVLVAFFISLNTT
                     TKFLVSVPLAQWFDAFLSELAANLFIGSAMLIAAVCARNAIPSLGIRQYAAVVFAVVI
                     AASCAEVGVELWTADGPLSAADDPWWAVPLIFASQIVRYSFVGLIIASAWLYVRAEAD
                     HVAAIAACAVDSERMARQTAEARLQMLEAQIEPHFLFNTLANVRRLYETDRTAGTTMM
                     RNLKSYLAVALPQMRSATCTLGRESDHAMAYLN"
     gene            complement(759..>1604)
                     /locus_tag="VFU90_11330"
     CDS             complement(759..>1604)
                     /locus_tag="VFU90_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480420.1"
                     /translation="VRCPVTHDFRDCKTGFTAFGGGNPHLEPETSEQFNAGVVWEPAS
                     GTSVSIDYWKIHKSNTILPLMPEQLFDNYARWAPTSVVRGPVDPAYPDLPGPIVSVLL
                     RNENLGDLRTAGFDVDLHWRGNAASLGRLSFELNGTYVTEYKLDFGTAQFSSGLGNNT
                     VTGGPVPRWRHYASVTWSNGPWSATLAQTFQSGYGECNQLTLEPDTGACTGTRRVGAY
                     DVWDIQGQYTGFRNTTIVAGIKNLFDRDPPFTQLGQGFSGGYEPVYADPRGVTYYAKL
                     AFAFK"
BASE COUNT          295 a          500 c          527 g          282 t
ORIGIN      
        1 cgtttagata cgccatggcg tggtcgcttt cccggccgag cgtgcaggta gcggaccgca
       61 tctgcggcag cgcgaccgcg aggtagctct tgaggttgcg catcatcgtc gtacctgccg
      121 tgcggtccgt ttcgtagagc cgtctgacgt tggcgagcgt gttgaacaga aaatgcggct
      181 cgatctgcgc ttcgagcatt tgcaggcgcg cctccgcggt ctgccgggcc atccgctccg
      241 agtccaccgc gcacgccgca atcgcggcga cgtggtcggc ctcggcgcgc acataaagcc
      301 aggcgctggc gatgatcagc ccgacgaacg agtaccggac gatctggctc gcgaagatca
      361 ggggcacggc ccaccaggga tcgtcagctg cggacagcgg cccatcggcg gtccatagct
      421 cgacgcccac ttcggcgcac gatgccgcga tcacgacggc gaacacgacg gccgcgtatt
      481 gccggatgcc gagcgacgga atcgcattgc gcgcgcaaac ggccgcgatc agcatcgcgc
      541 tgccgatgaa caggttcgcc gcgagctcgc tcaggaatgc gtcgaaccac tgcgcgagcg
      601 gcacgctgac gagaaacttg gttgtggtgt tgagcgagat gaagaacgca accaggacga
      661 cgatcgcaat tccttcgcga ccagggcgat cgagcaggcg cagcggcgag gtcgatcgcg
      721 catcgagcgt cgattctcct gacaagtcgc tcatttcgtc acttgaacgc gaacgcgagc
      781 tttgcgtagt aggtcacgcc gcgcggatcc gcgtagaccg gttcgtagcc gccgctgaat
      841 ccctggccca actgggtgaa cggcggatcg cgatcgaaca ggttcttgat gccggctacg
      901 atcgttgtgt tcctgaagcc ggtgtattgc ccctggatgt cccagacgtc gtacgcaccg
      961 acgcgacgcg tacctgtgca ggcgcccgta tcgggttcga gagtcagttg attgcactcg
     1021 ccgtaaccgg actggaaggt ctgggccagc gtcgccgacc acggcccgtt gctccacgtc
     1081 accgacgcat agtggcgcca gcgcggcacc gggccgccgg ttacggtgtt gttgcctagc
     1141 ccggagctga actgagcggt gccgaagtcg agcttgtatt cggtcacgta ggtgccgttc
     1201 agctcgaacg agagtcgacc gagggaagcc gcgttccctc gccaatgcag atcgacgtcg
     1261 aatcccgccg tccgcaggtc gccgaggttc tcgtttcgca gaaggaccga gacgataggc
     1321 ccaggcaagt cggggtacgc gggatcgacc ggacctcgca cgacgctcgt cggcgcccaa
     1381 cgggcatagt tgtcgaacaa ctgctccggc atcagcggca gaatcgtgtt gctcttgtgg
     1441 atcttccagt agtcgatcga gacagaagtg ccggaagcag gctcccacac cacgccggcg
     1501 ttgaattgct cggaagtctc gggttcgagg tgcgggtttc cgccaccgaa agccgtgaag
     1561 cccgtcttgc aatcgcggaa atcgtgggtc acggggcatc gcac
//
LOCUS       DASUCJ010000953         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1401106, whole genome shotgun sequence.
ACCESSION   DASUCJ010000953 DASUCJ010000000
VERSION     DASUCJ010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401106"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFU90_11335"
     CDS             complement(<1..1346)
                     /locus_tag="VFU90_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480421.1"
                     /translation="MATLPSATLSIKENAGAPATGSELVVILACVAQNADFLPRVMGS
                     TSDLLAEYTYSPGISYASTHIQKTGKPVMVVGMPITTPGAVTRQDTSGVTGSSLISIA
                     AGANGAMEECDGVVTVKRGGTVGTDQITLTVSLDGGESSQNVRLGTGNSYVIPYVGLT
                     LSFGAGTLNAGDVFKFNSTAPMWGSTAISSARAALAAQQKLARTWMVIGDLPSSTFAN
                     YVLNEANNYESTNDRFVTARAQVADRSPLPTTSANVHKTVNASLTFDSVGHTITRSGG
                     SWVTDGFAVGDVVSVTGSTSNNGSLGAITTLTATVMTFAAGLVAETTSVATVTGSPGL
                     VFAAAGHTITRSAGSFLTEGFAVGQNVTISGTASNNGNVGPISTLTATVMTFASGLTN
                     ETVGMGSASIVENLTMSGWMSASEAAFATIDASPRINLGAGRARVQDPITGYSFRRP"
     gene            complement(1353..1913)
                     /locus_tag="VFU90_11340"
     CDS             complement(1353..1913)
                     /locus_tag="VFU90_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480422.1"
                     /translation="MIHAIGEQLAAALAAAGIPLPVIDGPERRPTGTFARERIVLEPA
                     GDDRFESNHKATKNPKTKFSRVVSYKATLYVQAQSKGAMEWEHKVRAEALLDQVIIAL
                     EAIVKARANMWAPKSGKFVLPADLKDTETPGGAIYEFTFTIDRGITTDSWTGQAAPQV
                     TASAGFVTNQTNVTVNGTGTPETSCG"
     gene            complement(1910..>2349)
                     /locus_tag="VFU90_11345"
     CDS             complement(1910..>2349)
                     /locus_tag="VFU90_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480423.1"
                     /translation="EPVRPARRRRPAVSGPTSLKKFAEDLRRLPRVVAQKVAERSAPA
                     LTALAAATFDAGENAYGQAWAPGKGGKRVTLWRTGSLFKFIRYVAIGTKLRVALGVPY
                     AKYQVGKRPVFPRQDTALPDAYVQTLERTAVQVVKEELGGPAK"
BASE COUNT          378 a          795 c          765 g          411 t
ORIGIN      
        1 ggccggcgga acgagtagcc ggtgattgga tcctgcaccc gcgcgcgacc agcgccgagg
       61 ttgatccggg gcgacgcgtc gatggtcgca aacgcggcct cggacgcgga catccacccg
      121 gacatggtca ggttctcgac gatgctcgcc gagcccatgc ctacggtctc gttcgtgagc
      181 ccgctcgcga acgtcatgac ggtcgccgtc agcgtcgaga tcgggccaac gttcccgttg
      241 ttcgacgccg tgcccgagat cgtgacgttc tgcccgaccg cgaagccttc ggtcaagaag
      301 ctgcccgccg agcgcgtgat cgtgtgcccg gccgccgcga agacgagacc aggcgagccc
      361 gtcaccgtgg cgaccgacgt cgtctcggcg acgagtccag ccgcgaacgt catgacggtc
      421 gccgtcagcg tcgtgatcgc gccgagcgag ccgttgttcg acgtcgagcc ggtgaccgac
      481 acgacgtcac cgaccgcgaa gccgtccgtg acccacgacc cgcccgagcg cgtgatcgtg
      541 tgcccgacgc tgtcgaaggt caggctcgcg ttcaccgtct tgtgaacgtt cgccgaggtc
      601 gtcggcagcg gcgagcggtc ggcgacctgc gcgcgggccg tcacgaagcg atcgttcgtg
      661 ctctcgtaat tattcgcctc gttcagaacg tagttcgcga aggttgaact cggcagatcg
      721 ccgatgacca tccacgtgcg ggcgagcttc tgttgcgcag caagcgccgc acgcgccgag
      781 ctgatcgccg tcgagcccca cataggggcc gtcgagttga acttgaagac gtcgccggcg
      841 ttcagcgttc ccgcgccgaa gctcagcgtg aggccgacgt aggggatcac gtagctgttt
      901 cccgtcccga ggcgaacgtt ctgcgagctc tcgccgccgt cgagggaaac agtcagcgtg
      961 atctgatccg tgccgacggt cccgccgcgc ttcacggtca cgacgccgtc gcactcttcc
     1021 atcgcgccat tcgcgccggc ggcgatcgag atgaggctcg agcccgtgac accgctcgtg
     1081 tcctggcgcg tgacggcgcc gggggtcgtg atcggcatgc cgacgaccat gaccggcttc
     1141 cccgtcttct ggatgtgcgt cgatgcgtag gagatgcccg gcgagtacgt atactcggcc
     1201 agaagatccg acgtcgagcc catgacccgc ggcaggaagt cggcgttctg cgcgacgcac
     1261 gcgagaatca cgacgagctc ggacccggtc gccggggcgc cggcgttttc cttgatagac
     1321 agcgttgcag agggaagagt cgccatgaat ccttacccgc aagaggtttc gggcgtgccg
     1381 gtgccgttca ccgtcacgtt cgtttggttc gtcacgaagc ccgccgacgc cgtgacttgc
     1441 ggcgccgcct gacccgtcca agaatcggtc gtgatgccgc ggtcgatcgt gaacgtgaac
     1501 tcgtagatcg cgccgccggg cgtctcggtg tccttcaagt cagccggaag aacgaacttc
     1561 ccagacttcg gcgcccacat attcgcgcgg gccttcacga tcgcctcgag cgcgatgatt
     1621 acctgatcga gcaaggcctc ggcgcgtacc ttgtgctccc actccatcgc gcctttcgat
     1681 tgcgcctgaa cgtagagagt cgccttgtac gaaacgaccc gcgagaactt cgtcttcggg
     1741 ttcttcgtgg ccttgtggtt cgactcgaac cgatcgtcgc ccgcgggctc gagcacgatt
     1801 cgctctcgcg cgaatgtgcc cgtcggtcgc cgctcgggac cgtcgatgac cggaagcggg
     1861 atacctgccg ccgcgagcgc cgccgcgagc tgctctccga tcgcgtgaat catttcgcgg
     1921 ggccgccgag ttcttctttg acgacctgca cggccgtacg ttcgagcgtc tgcacatacg
     1981 cgtcaggaag cgcagtgtcc tgacgcggga agaccggccg cttaccgacc tgatacttcg
     2041 catagggcac gccgagcgcg acgcgcagct tcgtaccgat cgccacgtag cgaatgaact
     2101 tgaagagcga gccggtgcgc caaagcgtca cgcgcttgcc gcccttgccc ggggcccacg
     2161 cctgcccgta cgcgttctcg cctgcatcga acgttgccgc ggcgagggcc gtcagcgcag
     2221 gcgccgagcg ttcggcgacc ttctgcgcga cgacgcgcgg caaacgccga agatcctcgg
     2281 cgaacttctt cagagatgta gggccgctca cggcaggtcg tcgccgtcgc gcgggccgta
     2341 cgggctcac
//
LOCUS       DASUCJ010000954         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1492738, whole genome shotgun sequence.
ACCESSION   DASUCJ010000954 DASUCJ010000000
VERSION     DASUCJ010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492738"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFU90_11350"
     CDS             complement(<1..627)
                     /locus_tag="VFU90_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480424.1"
                     /translation="MVVGRVRVDLATCLTSKAPPVPTSVVTVRGTKNSQSILFKGKVV
                     LTLHERYGKDTPQGIPGPIVPLGISPDRKWILYAIDPMGSASLAADGLPLHAISVNGG
                     RSFPLPVVLAEDYRVWCGTRLVMTAGADRLSTNNKWLAVSKPPDWHTRTFLRDAHVAF
                     GSLVCDGNDVIVQSQPASTDANFFHSHWSLYRVGFSGAMTRLTSPPAGY"
     gene            681..>1231
                     /locus_tag="VFU90_11355"
     CDS             681..>1231
                     /locus_tag="VFU90_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5480425.1"
                     /translation="MAATDDRKTESGIPIEPVYGPERPGAYPFTRGPYPDMYRGRPWT
                     MRQYAGFASAEESNARYRYLLDRGQTGLSIAFDLPTQLGYDSDDPRALGEVGRTGVAI
                     DTIDDMRTAFDGIPLDAVSTSMTINAPASVLLCLYELVAEEQGVAPDALRGTVQNDIL
                     KEYVARGNYIFPPKPSMRLTTDL"
BASE COUNT          205 a          410 c          424 g          192 t
ORIGIN      
        1 gtagccggcc ggcgggctcg tgagcctcgt catcgcccca ctgaaaccga cgcgatagag
       61 gctccaatgc gagtggaaga agtttgcgtc cgtgctcgcg ggctgcgatt ggacgatgac
      121 gtcgttgccg tcgcagacga gggagccgaa tgcgacatgc gcgtcgcgca ggaacgtgcg
      181 agtgtgccaa tccggcggtt tgcttactgc cagccacttg ttgttcgtgg agaggcggtc
      241 ggcaccggcc gtcatcacga gccgggtgcc gcaccacacg cggtagtcct cggccagaac
      301 caccggcagc ggaaacgatc ggcctccgtt cacggagatt gcatggagcg gcagcccgtc
      361 cgccgcgagc gaggcggatc ccatcgggtc gatcgcgtac aggatccact tccggtccgg
      421 cgagatcccg agcggaacga tcgggccggg gatcccctgc ggtgtgtcct tgccgtaacg
      481 ctcgtggagc gtcagcacga ccttgccctt gaacaggatc gactggctgt tcttcgtccc
      541 gcgcacggtc accacgctcg tgggcacggg cggcgccttc gacgtcaggc aggtcgccag
      601 gtcgacccgc acccggccga cgaccacggc tccgagacct ggatgcgggg tgcagtggaa
      661 caactcgtac gattctgccg atggcggcaa ccgacgatcg gaaaacggag tcgggcatcc
      721 cgatcgagcc ggtgtacgga ccggagcggc cgggcgcgta tccgttcacg cgcgggccct
      781 atccggacat gtatcgcggc cgcccgtgga cgatgcggca atacgccggc ttcgcgtcgg
      841 cggaggagtc gaacgcgcgc tatcgctatc tgctcgaccg cgggcagacc ggcctgtcga
      901 tcgcgttcga tctgccgacg cagctcggct acgactcgga cgacccgcgc gcgctcggcg
      961 aggtcggccg caccggcgtc gcgatcgaca cgatcgacga catgcgcacc gcgttcgacg
     1021 ggatcccgct tgacgcggtc tcgacgtcga tgacgatcaa cgcgccggcg agcgtgctgc
     1081 tgtgcctcta cgagctggtc gccgaggagc agggcgtcgc gccggacgcg ctgcgcggaa
     1141 cggtgcagaa cgacatcctc aaggagtacg tcgcgcgcgg gaactacatc ttcccgccga
     1201 agccgtccat gcggctgacc accgacctgt t
//
LOCUS       DASUCJ010000955         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1186837, whole genome shotgun sequence.
ACCESSION   DASUCJ010000955 DASUCJ010000000
VERSION     DASUCJ010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186837"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..582)
                     /locus_tag="VFU90_11360"
     CDS             complement(64..582)
                     /locus_tag="VFU90_11360"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5480426.1"
                     /translation="MASTTSTNRTTLVSIVIILATAAAGCSGGGFLPFGDGPHSIAPA
                     VLNSIIARQSEEHGVSPALVRAVIHQESGEDPSAISSAGAMGLMQLMPGTADAYGVAD
                     PFDPEQNVAGGTALLADLLRQYHGNVRLSLAAYNAGSGAVAKYHGVPPFAETQAYVRD
                     VTSLYRASKSHP"
     gene            926..>1214
                     /locus_tag="VFU90_11365"
     CDS             926..>1214
                     /locus_tag="VFU90_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480427.1"
                     /translation="MRANGIVHRALAGLGTAIALARLAARDATGRLVFEALLGVEVLL
                     TRCEDELRSAIPAGQRSVLIGHWNVPRNSAIWKRKNWPRPMTEDRKRRMTLT"
BASE COUNT          218 a          380 c          422 g          194 t
ORIGIN      
        1 tcggtcaccg ggcgcaggct tgtggcgaac gccggaaggc cggggcgagc gctgtccatg
       61 accttacgga tgcgacttcg aggcgcgata gagcgacgtc acgtcgcgca cgtacgcctg
      121 cgtctcggcg aacggcggca cgccgtgata cttcgcgacc gcgccggaac cggcgttgta
      181 cgcggcaagc gaaagacgca cgttgccgtg atactggcgc agcagatctg ccagcagcgc
      241 cgtgccgccc gcgacgttct gctcggggtc gaacggatcg gcgacgccgt acgcgtcggc
      301 ggttcccggc atcagctgca tgagtcccat cgcgccggcg gatgagatcg cagacggatc
      361 ctcgccggat tcctggtgga tgacggcgcg gacgagcgcg ggcgaaacgc cgtgctcctc
      421 ggattggcgc gcgatgatcg agttgaggac cgccggcgcg atcgagtgag ggccgtcacc
      481 gaaggggagg aaaccgccgc cgctgcagcc ggcggccgcc gtcgctagga tgatgacgat
      541 gctgacgagg gtggtccggt tcgtcgaggt cgtggacgcc atccggatgg caactcctgt
      601 cccaaaaagc ggtctgctcg gctgcgtcga ccgcctccgg cgtatagcaa tagtatcggc
      661 taaccgtcgc cccgaaaagg ggctttcggt gagagcggga ttgggccccc cgcacgggct
      721 gccgcacggc tggtaagcgc ccggccgggg atgccgaaaa ggccgcatat caaacggccg
      781 agagatgcga cctggaacgc gccccgatca gggacggatc atattacact cgcgcgcgtc
      841 tgccggcgac ggtccggtag cagtccgggc aatataccgg tttgtccggg cgcggtgaga
      901 acggcacttg tgtcgcggca ccgcattgcg tgcaaacggc atcgtacatc gcgcgctcgc
      961 cggtctggga accgcgatcg cgcttgcgcg cctggcggca cgcgacgcaa cgggtcggct
     1021 cgtgttcgaa gcccttctgg gcgtagaagt cctgctcacc cgctgtgaag acgaactccg
     1081 ctccgcaatc ccggcaggtc agcgttcggt cttgataggc cactggaatg ttcctcgaaa
     1141 ttccgccatt tggaaacgca agaattggcc gcggccaatg acggaagacc gcaagcgtag
     1201 gatgactcta acat
//
LOCUS       DASUCJ010000956         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_743841, whole genome shotgun sequence.
ACCESSION   DASUCJ010000956 DASUCJ010000000
VERSION     DASUCJ010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743841"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..587)
                     /locus_tag="VFU90_11370"
     CDS             complement(3..587)
                     /locus_tag="VFU90_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480428.1"
                     /translation="MKRVPAAGDSISMIAAPFAMPTCLAELSGMASARRRHRDADVIV
                     IGAGAAGLAAANVLLDNGLDVLVLEARRRTGGRIETAALLPSGSPIELGAEFLHGVAA
                     PIREIAREHDFRTIDISGQRFEHTTHGLRVARDYTARLDRVLGRLDAHRTPDRSFADA
                     MRANRGSLRASDRSLATRYVEGFEAADATLIGER"
     gene            complement(633..1616)
                     /locus_tag="VFU90_11375"
     CDS             complement(633..1616)
                     /locus_tag="VFU90_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5480429.1"
                     /translation="MMHRTFAASLAAVCCTVGLLRPATLVGQQPEQGYITTSDSARLF
                     YKIAGRPGPGVDTIIAIHGGPGLDLESLYNDFAPALGRKHVVIFYDQRGGGKSELPAD
                     TTRLVAARQIQDLDEVRRHFGLTQVTLVAHSYGPLLAASYAIAHPDAVKKLVFFGSVP
                     PRRGDFFTRYGQNLNARLDSVQRTSLARLDSIMSSDSASDDVVRTACRKFWAIGLRPR
                     LASPHGRRAVKADLCATDPKGIRYGSKTANHVIMNSYGDWDLRPSLRTLDVPLLVVHG
                     REETIPMDLVEEWVTSMSHARLFKVPNAAHFTYAEQPGLVWPVVERFLARR"
BASE COUNT          314 a          637 c          634 g          303 t
ORIGIN      
        1 agtcagcgct cgccgatcag cgtcgcgtcg gcggcctcga atccctccac gtagcgcgtc
       61 gcgagcgacc ggtcggacgc gcgcagcgac ccgcgattcg cgcgcatcgc gtccgcgaac
      121 gaccggtccg gcgtgcggtg cgcgtcgagg cggccgagca cacggtcgag gcgcgccgtg
      181 taatcgcgcg cgacgcgcaa cccgtgcgtc gtgtgctcga atcgctgacc cgaaatatcg
      241 atcgtgcgaa agtcatgttc tcgtgcgatc tcgcggatcg gtgcggccac gccgtgcagg
      301 aattccgcgc caagctcgat cggactgccc gatggcagca gcgcggctgt ctcgatgcgc
      361 ccgccggtgc gacgtcgcgc ctcgaggacg aggacgtcga gcccgttgtc gaggaggacg
      421 ttcgcagcag caagacccgc cgcgccggcg ccgatgacga tgacatcggc gtcgcgatgc
      481 cggcgccgag cgctagccat gccgctgagc tcggccagac atgtcggcat cgcgaacggc
      541 gcggcgatca tcgatatgga atcgcctgcg gccggaacgc gtttcacgtc gatgaccgaa
      601 ggcaatcatg cgcgcggggt cggcagtcat tctcacctcc gggcgaggaa ccgctcgacc
      661 accggccaca ccagcccggg ctgctcggcg taggtgaaat gcgctgcgtt aggcaccttg
      721 aagagcctgg cgtgcgacat cgaagtcacc cactcctcca ccaggtccat cggaatcgtt
      781 tcctctcggc catggaccac gagcagcggc acgtccagcg tccggagcga tggacgcaga
      841 tcccagtcgc cgtacgaatt catgatgacg tggttggccg tcttgctgcc gtagcggatg
      901 cccttcgggt ccgtcgcgca cagatcggct ttcacggcgc gccgtccgtg aggcgacgcc
      961 agccgcgggc gcaggccgat cgcccagaac ttccggcacg ccgttcggac cacgtcgtcg
     1021 gatgcgctat cgctcgacat gatggaatcc agccgcgcaa gcgatgtccg ctgcacgctg
     1081 tcgaggcgcg cattgagatt ctgcccgtag cgggtgaaga agtcgccgcg ccgcggcggc
     1141 accgagccga agaacaccag cttcttcacg gcatccggat gcgcgatggc gtagctggcc
     1201 gcgagcagcg gtccgtacga gtgtgcgacc agcgtcactt gcgttaggcc gaagtgccgc
     1261 cggacctcgt cgaggtcctg gatctgacgc gccgccacga ggcgcgtcgt gtccgccggg
     1321 agctccgact tcccgccccc gcgctgatcg taaaagatca ccacgtgctt gcggccgagt
     1381 gccggcgcga agtcgttgta cagcgactcg aggtcgaggc cggggccgcc gtggatcgcg
     1441 atgatcgtgt ccacccccgg acccggccgg cccgcaatct tatagaagag ccgcgcagaa
     1501 tccgacgtcg tgatgtagcc ctgctccggc tgctggccga cgagggtcgc cggacggagg
     1561 agaccgacgg tgcagcaaac ggcggcgagg ctggcggcaa acgtgcgatg catcatgctc
     1621 ggtacgcgtc gagatgggag cgacgaatct agctccgccc tcccgtttcg ccacccgtac
     1681 gctgcgcccg ttgcgaacca ttcccgccgg aagccgcgac ggagtcacat tcggctatcc
     1741 gttgtgactc ttgcgcgtga acgtcgccat cgtcttcggc gcgtgctcga tcaaggcgat
     1801 gtcggtgcgc agcgcggcga gatcgaaacc gcgcctggtc ttgtcgctct tcgagatgaa
     1861 cgcgtcgctg agcacggcgt acgattca
//
LOCUS       DASUCJ010000957         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_254603, whole genome shotgun sequence.
ACCESSION   DASUCJ010000957 DASUCJ010000000
VERSION     DASUCJ010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254603"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1035)
                     /gene="mtnA"
                     /locus_tag="VFU90_11380"
     CDS             complement(16..1035)
                     /gene="mtnA"
                     /locus_tag="VFU90_11380"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEU5480430.1"
                     /translation="MLYGAGAGRGVNDVTAVAWDGDAVVYLDQRLLPHEVRYERARTL
                     EEIESAIASLAVRGAPCIGVFGAFGVALLRQSIGDDAEFARAAQRVREARPTAVNLAW
                     AVDRVLRASDMLDEAHAIAAEQVRVDAAIGENGLELIPKNARVLTHCNTGPLATAGGG
                     GTALAVILAAQRGGKHPRVFVDETRPLLQGSRLNYFELQNAGVDAVLQPDAAAASAMA
                     RCGVDLVIVGADRIARNGDTANKIGTYGLAILAAHHKIPFYVAAPRSTFDFSISNGDQ
                     IPIEERPAGEVTEVHGRRVAPPGAHVYNPAFDVTPAHLIAAFVTERGILRPPFTESIR
                     DLEPA"
     gene            1480..>2245
                     /locus_tag="VFU90_11385"
     CDS             1480..>2245
                     /locus_tag="VFU90_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480431.1"
                     /translation="MKNKSFAALIVGVTFAASAVATAIPAEAQVYVGVGFGPSVVAYA
                     PSPAPVQPYCTPYSYNQWIWQPGYGYVWCPVAPPPAYVPPASYVPAYSYAPAYSYAPA
                     YPYAPAYSYAPAYTYAPPPVYPAYSSSALIGTAVGLGVLGLIAAAANQPLYAPYPSLY
                     AGYAPYPSLYAGFAPYPSLYAGYAPIPSLYAAYSYYPGYAMAAANPGFAVAQAPATVY
                     NVNHVTRVYRTTIVRRNGTVIRRRVAMPARFARTMHP"
BASE COUNT          361 a          773 c          712 g          399 t
ORIGIN      
        1 cgaaatcgta atacttcacg ccggctcgag atcgcgaatc gattcggtaa acggcggccg
       61 gaggatgccc cgctcggtaa cgaacgcggc gatcaaatgc gccggcgtca cgtcgaacgc
      121 agggttgtag acgtgcgcgc ccggcggcgc cacccgccgc ccgtgcacct ccgtaacttc
      181 gccggccggg cgttcctcaa tcggaatttg gtcgccgttc gaaatcgaaa aatcgaacgt
      241 cgaacggggc gccgcaacgt agaacggaat cttgtggtgc gccgccagga ttgccagacc
      301 gtacgttccg atcttgttgg ccgtgtcgcc gttgcgcgcg atccggtcgg cgccgacgat
      361 caccaagtcg actccgcagc gcgccatcgc gctggccgcc gccgcatcgg gctgcagcac
      421 tgcatcgacg ccggcatttt gcagctcgaa gtagttcaag cgggaaccct gcagcagcgg
      481 ccgcgtttcg tcgacgaaca cgcgcggatg cttcccgccg cgctgcgccg ccaggataac
      541 cgctagcgcc gtcccgccgc cgccggcggt ggccagcgga ccggtgttgc agtgcgtgag
      601 aacccgcgcg ttttttggaa tcagctcgag gccgttctca ccgatggccg cgtcgacccg
      661 cacctgttcg gccgcgatgg cgtgcgcttc gtcgagcatg tcggatgcgc gcagcacgcg
      721 gtcgaccgcc cacgcgagat ttaccgcggt ggggcgcgcc tcacgcacgc gctgcgcggc
      781 tcgcgcgaac tcggcgtcgt cgccgatcga ttgccgcagc agcgcgacgc cgaacgcgcc
      841 gaaaacgccg atgcacggag cgccgcgcac ggctaagctc gcgatcgcgc tctcaatctc
      901 ttcgagcgtc cgggcgcgct cgtagcgcac ctcgtgggga agcagccgct gatcgaggta
      961 aacgacggcg tcgccgtccc aggcgaccgc cgtgacgtcg ttcacacccc ggccggcacc
     1021 tgcgccgtaa agcacgtgcg gcaatagacc gggcgatcgc cgcgtggccg gaacggcaac
     1081 gtcgtctcga ccccgcaagc cgcgcagacg gcttcatagt tggtgcgtgc cggtcgcggc
     1141 gggtgtgcct ccccgccgct cgctcgccgg gcttggcggc aatcgcggca acgttgcggc
     1201 tggttttgga accccttctc ggcaaaaaac tgctgctctc gcgccgagaa tatgaactga
     1261 ttggcgcagt ccttgcaggt aagcgtcttg tcttccaacg tcgggacctc cacacgcagg
     1321 ctaaggcgcc ttaatgcgac ccccaccagg gagttcctcc gctcccttgg ccgctagggg
     1381 ccgctaggtc gctttcccct tctaatagga ccctaattcc caagcgagca cgcggcggca
     1441 ttacgggtaa taatggagag cgagagggga agacttctta tgaaaaacaa gtcgtttgca
     1501 gcgctgatcg tcggggtgac cttcgcagca tcggctgtcg cgaccgcgat tccggccgag
     1561 gctcaggtgt acgtcggcgt cggcttcggt cccagcgtag tagcgtacgc gccgtcgccg
     1621 gctccagtgc agccgtattg tacgccgtat agctacaacc agtggatttg gcagccgggc
     1681 tacggctacg tgtggtgtcc ggtagcgccg ccgcccgcgt acgtcccgcc ggcatcgtac
     1741 gtcccggcgt attcgtacgc accggcgtat tcgtacgcac cggcatatcc gtacgcaccg
     1801 gcgtatagct acgcgcccgc gtatacctac gctccacccc ccgtgtatcc ggcgtactcc
     1861 agcagcgcgc tgatcggtac cgcggtcgga ctgggcgttc tcggcttgat cgcggccgcc
     1921 gcgaatcagc cgctgtacgc gccgtatccg tcgctctatg ctggatacgc gccgtatccg
     1981 tcgctctatg cgggattcgc gccgtatccg tcgctctacg cgggatacgc gccgattccg
     2041 tcgctctacg cggcgtactc ctactatccg ggctacgcga tggctgcagc caatccgggc
     2101 tttgccgtcg cccaagctcc ggccacggtc tacaacgtga accacgttac gcgcgtgtat
     2161 cgcacgacga tcgtgcggcg taacggcacg gtgatccgtc gccgggtggc gatgccggca
     2221 cgcttcgcgc ggaccatgca tccgt
//
LOCUS       DASUCJ010000958         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1523286, whole genome shotgun sequence.
ACCESSION   DASUCJ010000958 DASUCJ010000000
VERSION     DASUCJ010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523286"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..964)
                     /locus_tag="VFU90_11390"
     CDS             complement(170..964)
                     /locus_tag="VFU90_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480432.1"
                     /translation="MTPTMNGTPLHARNVTLSSRLRAGAFACGLCASFAASLAWADPD
                     DYVSTPAVEYGERELELRYGTATDPGGSRRDAGSIAFGYGATPWWFTEVYAKFQRAGG
                     GSTHYDAVEWENKFQLTEPGEYPVDMGLLVEIEAPHDRAEGYELKLGPLFQKDFGLVQ
                     VNANAFLAHHVRSSEPGATDLSYQWQAKYRWREALEFGAQGFGEVGKWDNWDPAHEQK
                     HIAGPALFGKVGVGGRQVINWNAGLLFKLSNAAPDRTFRAQLEVEF"
     gene            complement(961..1275)
                     /locus_tag="VFU90_11395"
     CDS             complement(961..1275)
                     /locus_tag="VFU90_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5480433.1"
                     /translation="MLLAVVLLSGVANAAATEPEFALVVRDHRFVPDEIVIPAGTRVR
                     IVVENQDTTPEEFDSHALNREKMIVGRSKATIYIGPLSSGRYEFIGEFNAATARGVVI
                     VK"
     gene            1381..2064
                     /locus_tag="VFU90_11400"
     CDS             1381..2064
                     /locus_tag="VFU90_11400"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU5480434.1"
                     /translation="MAGMRIEQATGIGGMNDNYSHSHNARPPTWCAVAAPYTMTAVPT
                     MKLSRHFLIAMPAMADPNFAHTLAWICEHNDEGALGIVVNRPTDMTLSALFEHMEIPL
                     GDSHLRTASVHFGGPVQIDRGFVLHRPIGHWQSTIEVAEGVGLTTSKDVLEAAARGEG
                     PKDMLVSLGYAGWSAGQLEHELAQNAWLTVEADPDVVFDLPAERRLPAAMKLLGIDMS
                     RLSDNVGHA"
BASE COUNT          359 a          723 c          718 g          368 t
ORIGIN      
        1 cggcgtgtgc ccgccggaga gcgcgatcga gcacgctccg cgcttcgcga tcgcgacggc
       61 gatcgcgtcg acgatccacg cggccgcgat ttcggcgagc gcctgctcag tcgcgacgac
      121 gagcggtccg gcaggtgttg ggcgcatcgc aacccctcct gtggagggat cagaattcga
      181 cctcgagctg cgcgcgaaac gtccgatccg gagccgcatt cgacagtttg aagaggagac
      241 ccgcgttcca gttgatcacc tgccgcccac cgaccccgac cttcccgaac agcgccggac
      301 ccgcgatgtg cttctgctcg tgggccggat cccagttgtc ccatttgccg acctcgccga
      361 atccctgtgc gccgaactcg agggcctcgc gccagcgata cttcgcctgc cattgatacg
      421 acaggtcggt cgccccgggt tcgctcgagc ggacatggtg cgcgaggaag gcattggcgt
      481 tcacctggac gaggccgaag tccttctgga acagcgggcc gagcttcaat tcgtaaccct
      541 cggcgcggtc gtggggagcc tcgatctcga ccagcaatcc catgtcgacc gggtattcgc
      601 ccggctcggt caactggaac ttgttctccc attccaccgc atcgtaatgc gtcgatccgc
      661 cccccgcgcg ttggaacttg gcgtagacct cggtgaacca ccacggtgtc gcgccgtatc
      721 cgaacgcgat cgagccggca tcccggcggc tgccgccggg gtcggtcgcg gtgccgtagc
      781 ggagctccag ctcgcgctca ccatactcca cggcgggcgt cgagacatag tcgtctggat
      841 ccgcccaggc gagcgacgcg gcgaagctcg cacagagtcc gcaggcaaac gcgccggcgc
      901 gcagccgcga cgacaacgtg acgtttcggg cgtgtagcgg cgtgccgttc atggtcggcg
      961 tcacttcacg atcaccacgc cgcgcgcggt ggcggcattg aactcgccga tgaactcgta
     1021 gcgtccggag gagagcggtc cgatgtagat cgtcgctttc gaccgcccca cgatcatctt
     1081 ctcgcggttg agcgcgtgac tgtcgaactc ctcaggagtg gtgtcctgat tctcgaccac
     1141 gatccggacg cgggttccgg ccgggatcac gatctcgtcg ggtacgaagc gatgatcgcg
     1201 cacgaccagc gcgaattcgg gctccgttgc ggcggcattg gcgaccccgc tgaggaggac
     1261 aaccgcaagc agcacggcgg cgagtcgagg cgttcgggac gcgagtgatc gtggggactg
     1321 cgcgaattgc gtcgggtcag ggaaggacat ggatcggtct cgggtgcggt tcggttggag
     1381 gtggccggaa tgcgaatcga gcaagccaca gggatcggcg gcatgaatga taactattct
     1441 cattcgcata acgcaagacc cccgacttgg tgcgccgtcg cggcgcctta tacaatgacg
     1501 gcagtgccga caatgaagct cagccgccac ttcctgatcg ccatgccggc catggccgat
     1561 ccgaatttcg cgcacacgct cgcgtggatc tgcgagcaca acgacgaggg cgcgctcggc
     1621 atcgtcgtca accgtccgac cgacatgacg ctctcggcgc tcttcgagca catggagatc
     1681 ccgctcggcg acagccacct gcgtacggcg tcggtgcatt tcggtggccc ggtgcagatc
     1741 gatcgcggct tcgtgctgca ccggcccatc ggccactggc agtcgacgat cgaggtcgcc
     1801 gaaggcgtcg gcctcacgac gtcgaaggac gtcctcgaag cggccgcgcg cggcgaggga
     1861 ccgaaggata tgctggtgtc actcggctac gccggctggt cggccgggca gctcgagcac
     1921 gagctcgccc agaatgcgtg gctcactgtc gaggccgatc ccgacgtcgt gttcgacctt
     1981 cctgccgaga ggcgcctgcc ggcggcgatg aagctcctcg gcatcgacat gtcgcgcctc
     2041 tcggacaacg ttggtcacgc gtgacgaagc cccggcgcgg ggccgacgcg acgctcttcg
     2101 cgttcgattt cgggacgcgc cgcatcggtg tcgcggtcgg cagcacgctg acccgcgtcg
     2161 cgcatccg
//
LOCUS       DASUCJ010000959         4424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1049488, whole genome shotgun sequence.
ACCESSION   DASUCJ010000959 DASUCJ010000000
VERSION     DASUCJ010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049488"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VFU90_11405"
     CDS             complement(<1..1371)
                     /locus_tag="VFU90_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEU5480435.1"
                     /translation="MELRVRYLAPAPAFMAVLAFGVLAACGNHGQQRFLPNLHTARRA
                     HQLTQDPYATAILNDSPTVYYRLDDTGTIAADSSGGGHNATVGSSVVESASGLLTSSQ
                     DTAMSFPGSKTAAGIVSTAAIPALQPTAAVSLEVWMQFGTTPALFTTAVAYGNDSNYA
                     PYDLYFSGGGKLAAQFYLNGGVLVATSPAALKANTIYHVVSTYDGTTAKLYVNGALVS
                     SATKTGTLAGYDSHGVGIGDDAAVSNPAFKGTLDEVAIYAGKTLTATQVLNHYNAGIG
                     NGPPPTATPPPTATPPPTPPPTPSPTPSTNGGYSTAVLNDRPTVYYRLDDTGTTAKDA
                     SGNGLSATIGGAVTTNAPGLLVSSNDTAMTFPGTASLAGVVAASANALLQPSSSVSIE
                     GWLRFSTAPRTYTVPVGYGNDNNYAPYDWYFGSGGKLIAQFYLSSGPLLVVSPAAMQP
                     NTTYYVV"
     gene            1580..1930
                     /locus_tag="VFU90_11410"
     CDS             1580..1930
                     /locus_tag="VFU90_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480436.1"
                     /translation="MKNMHTALAALVLTFATLAPALALVYDGKLPLYPRATIFVLDAG
                     KPQSYWPTGLRDGDPSGSKTTDSVSAVTAWYREHLPGYALHTSAEGAVFSGPGGLVHI
                     ASLHGTTIIALNPS"
     gene            1998..2822
                     /locus_tag="VFU90_11415"
     CDS             1998..2822
                     /locus_tag="VFU90_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480437.1"
                     /translation="MWTLVGFVVASAIGLTAVLSFGRRGSIRRRDYPWMLEGVSQAHV
                     TIVGTLAGFAFTGVVLVVTLAHDRPGRAEISFDTVVVMFLVSYLYWVGSAFLISYLPH
                     AGTSGDLVQRVHFSLATTIEYRTVFLSWFALLPLLQANGLGRLVPVLYFLLPASLLLG
                     SLLVGMTTDGLGLIAIKEIYLSAAVGTGLALGYGMIVAFALPVLRSPYSSLYLALVVF
                     CINGIGFALAAGTPLVSQYEGIKRFYERYGRKLIIADMQVTAVSLAFLWLAVVGVI"
     gene            complement(2839..>4424)
                     /locus_tag="VFU90_11420"
     CDS             complement(2839..>4424)
                     /locus_tag="VFU90_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5480438.1"
                     /translation="GARPRCACEKVGRADERSSMNDLTNGVERVSVLVVGAGPTGLLL
                     AGELQRRGVSCKLIDARPGPLHWDRATVIHPRSLELFESLGLIDRFLEAGTKQRGVKM
                     YSAGQLLGALDLAECGSRYGFNLGLSEEATESILTDYLDALGGSVHRSARLIGASAHD
                     GGLLAEIEQGGRHCRVDAEWIVGCDGAHSPTRELGGIVLEGHDIADPWAVFDAAIRGW
                     TDDFELNVGYLDAIPVILTALPDRRWRVYLRPSSSESDLVDDAASTLRQYAATASFAD
                     IANPTRFHCSSKVANRFRSGRVLLAGDAAHLCSPAQGHGMNSGLQDASNLGWKLALVC
                     EGVADPSLLDSYGAERQPIAKMVTQSGDLTEQGQMLTDPAKRRDRDATIRAMFADPEA
                     RHNEIVAEAELNADYAASPIVFGTGGGLAAGQRAPGTIPVRSAGHTLVLAGDATSEDR
                     EFAALHDALQKAVRSSRVFEAIVTVSANDAFGNTGRHGGTTLLAVRPDGYVGMLSEAN
                     YLTAVERYHALVTARRTAP"
BASE COUNT          777 a         1374 c         1391 g          882 t
ORIGIN      
        1 aaccacgtaa tacgtggtgt tcggctgcat tgcggcaggc gacacgacca gcagcggacc
       61 ggaactcaag tagaactgcg caatcagttt gccgcccgaa ccgaagtacc agtcgtacgg
      121 cgcgtagttg ttgtcgttac catacccgac cggcaccgta tacgttctcg gcgcagtcga
      181 gaatcgaagc cagccttcga tcgaaaccga acttgagggc tgcaggagcg cgttcgcact
      241 tgcggcgacg acgccggcca gactcgcggt accggggaag gtcatcgccg tatcgttgct
      301 cgagaccagc agtcccggag cgttcgtggt taccgcgcct ccgatggtcg cgctcaagcc
      361 gttgcccgaa gcgtcctttg ccgtcgttcc ggtatcgtcg agacgatagt agacggtcgg
      421 tcgatcgttc agcaccgccg tcgaatatcc accgttcgta ctgggggtag gcgaaggcgt
      481 aggcgggggc gtcggcggcg gcgttgcggt tggcggcggc gttgcggttg gcggcgggcc
      541 gttgcctata cccgcattgt aatggttcaa tacctgcgtc gccgtcagcg ttttgccggc
      601 gtaaatcgcg acttcgtcaa gcgttccttt aaaggctgga ttgcttacag ccgcatcgtc
      661 gccgatcccg acgccgtgtg agtcgtatcc cgccagcgtt ccggtttttg tagcgctcga
      721 caccagcgcg ccattgacgt acagcttggc ggtcgtgccg tcgtaggtcg acacgacgtg
      781 atagatcgtg ttcgctttca gcgcggccgg cgaggtcgcc acgagcacgc cgccgtttaa
      841 gtaaaattgc gcggcgagct tgccgccgcc ggaaaaatac aagtcgtacg gcgcgtagtt
      901 cgaatcattc ccgtacgcaa ccgccgtcgt aaaaagtgcc ggagtcgtgc cgaattgcat
      961 ccagacttcg agagataccg cagccgtagg ctgaagcgcg ggaatcgcgg cggtcgacac
     1021 gatccccgcg gccgtttttg accccgggaa gctcatggcc gtgtcctgac tcgacgtgag
     1081 gagtcctgaa gcagattcta cgacgctgct tcccaccgtg gcgttgtggc cgccgccgga
     1141 ggagtccgca gcgatcgttc cggtgtcgtc cagccgatag tacacggtgg gtgagtcgtt
     1201 taagatcgca gtcgcatagg gatcctgcgt gagctgatgt gcccggcgag cggtgtggag
     1261 gttcggaaga aacctctgct ggccgtgatt accgcacgct gcgagaacgc cgaaggcgag
     1321 gaccgccata aaggccggag ccggagctaa gtaacgcacg cgcaactcca ctcgtgagaa
     1381 agctagcact cttatactag gctgcccgtt tttaggccgg taggggaaga aggtcccaaa
     1441 tacggcgtga ccaattaccc acgtctcgtc agccgaggag gcgacgttgc ttcggcgatc
     1501 ggagtcccag ccgttgccgt ccgggtctcc gcgcagggtg acgcacgcgg gcggcacgcg
     1561 tcgcccttac gatacggcca tgaagaacat gcataccgcg ctagcggctt tggtcctcac
     1621 gttcgcgacc ctcgcacctg cgctggccct cgtctacgac ggaaaacttc cgctgtatcc
     1681 gcgtgccacc atcttcgtgc tcgatgcggg caaaccgcaa tcgtactggc ccacggggct
     1741 gcgtgacggc gatccctccg gctcgaagac gaccgattcg gtaagcgcgg tcaccgcctg
     1801 gtatcgcgag caccttccgg gatacgcgct gcacacgtcg gcggaaggcg cggtgttctc
     1861 cggacccggc ggcctcgtcc acatcgcttc gcttcacgga acgaccatta tcgccctcaa
     1921 tccaagctga agcccgaagc ctctcggcaa agaggctcgg cggcgaggcg cagcatacta
     1981 cttcattcga aacggcaatg tggacgctcg taggtttcgt ggtcgcttcg gcgatcggtt
     2041 tgacggccgt gctgagcttt gggcgtcgcg gttcgatccg gcgccgcgac tacccatgga
     2101 tgctggaagg ggtttcccag gctcacgtca cgatcgtcgg aactctagcc ggctttgcat
     2161 ttacgggcgt cgtgctcgtc gtaacgctcg cacacgacag gccgggccgc gccgaaattt
     2221 ccttcgacac ggtcgtcgtg atgttcttag tttcgtacct gtactgggtg gggagcgcgt
     2281 ttctgatctc atacttgccg cacgcgggca cgagtggaga tctcgttcag cgcgttcatt
     2341 tcagtctggc cacgacgatc gaataccgca ccgtatttct gtcgtggttc gcactactgc
     2401 cgctgcttca ggcgaacggc ctcgggcggc tggttcccgt tctctatttc ttgctgcccg
     2461 cgtcgctgct tctgggctcg ctgctggtcg gtatgacgac cgatggtctg gggctcatcg
     2521 ctattaaaga aatctatctc tcggcggccg ttggcacggg actcgcactg ggttacggga
     2581 tgatcgtggc gttcgcgctt cccgtattgc gctcgccgta ttcctcgctg tatcttgcgc
     2641 tcgtcgtatt ttgcatcaac ggcatcggct tcgcactggc tgcaggaacg ccgctcgtgt
     2701 cgcaatacga gggcatcaaa cgtttctacg agcgctacgg gcggaagctt atcatcgcgg
     2761 acatgcaggt gacggcggtt tcactagcct ttctctggct agccgtggtc ggagtcatct
     2821 gagcgagcgt acacgagtct aaggggcggt acgccgcgcc gttacgagcg cgtggtaccg
     2881 ctcgaccgcg gttagataat ttgcttccga cagcatgccg acgtatccgt cggggcgcac
     2941 cgccaggagc gtggtgccgc catgcctgcc ggtattgccg aacgcgtcgt tcgcactcac
     3001 agtgacgatg gcttcgaaca cgcgcgagct gcggaccgct ttttgcaaag cgtcgtgaag
     3061 ggcggcaaac tcgcggtctt cgctcgtcgc atcgcccgcc agcacgaggg tatggccggc
     3121 cgaccgaacc ggtatcgtgc cgggggcgcg ttggcccgcg gcgagaccgc cgccggtgcc
     3181 gaaaacgatc ggcgacgcgg cgtaatccgc attcagctcg gcttccgcaa cgatttcgtt
     3241 atgccgcgct tccgggtcgg caaacatcgc gcggatcgtc gcatcgcgat cccggcgttt
     3301 cgccgggtcc gtaagcattt gcccctgttc ggtcaggtcg cccgattgcg tcaccatttt
     3361 agcaattggt tgccgctcgg ccccatagct atcgagaagc gaaggatccg cgacgccttc
     3421 gcaaacgagc gcgagtttcc aacccagatt agaggcgtct tgcagcccgg agttcatgcc
     3481 gtgtccctga gccggcgaac ataggtgtgc ggcatcgccc gcgagcaaca cgcgtcccga
     3541 acgaaatcga ttggcaacct tgctggaaca atgaaaccgc gtgggattcg cgatatcggc
     3601 gaacgacgcc gtcgctgcgt actgccgcag cgtcgatgcg gcgtcgtcga cgagatccga
     3661 ctccgacgag ctgggccgca ggtaaacgcg ccatcgccga tcggggagtg cggtcaaaat
     3721 gacggggatc gcatcgaggt aaccgacgtt gagttcgaag tcatcggtcc agccgcgaat
     3781 cgcggcgtcg aagaccgccc aaggatcggc aatatcgtgc ccttccagaa cgatgccgcc
     3841 cagctcgcgg gtcggactgt gggctccgtc gcagcccacg atccattcgg cgtcaacccg
     3901 gcagtggcgg ccgccctgtt cgatctcggc gagcaagcct ccatcgtgag cgcttgcccc
     3961 tatgaggcga gccgatcggt gtacgctgcc tccgagcgcg tccaagtaat cggtgaggat
     4021 cgattcggtg gcctcttcag acagtccgag gttgaacccg tagcggcttc cacactccgc
     4081 taggtcgagc gcaccgagca gctgacccgc cgagtacatc ttgacgccgc gctgtttcgt
     4141 gcccgcttcg agaaatcggt cgatgagacc aagcgattca aagagctcga gcgagcgagg
     4201 atgaatcacg gtcgcgcggt cccagtggag gggtccgggc cgcgcgtcga tgagtttgca
     4261 cgacacgcct cgcctttgaa gttctccggc caggagcagg cccgtcggcc ccgcgcccac
     4321 gacgagtacc gatacgcgtt ctacaccgtt cgtgagatcg ttcatgctcg agcgctcgtc
     4381 agctcggccg actttttcgc acgcgcaacg aggccgggcg cccc
//
LOCUS       DASUCJ010000960         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_835378, whole genome shotgun sequence.
ACCESSION   DASUCJ010000960 DASUCJ010000000
VERSION     DASUCJ010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835378"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFU90_11425"
     CDS             <1..998
                     /locus_tag="VFU90_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480439.1"
                     /translation="LKAGAYQNWWYLNGWAPAVSSSNKGTIVPFTRTVGSFDPHLALT
                     YQPRGGESFRLAAGTSTTFPYAGIVSGTAYTTPGATGTVALNGKNPLLSPEKAYEADL
                     GFDKRMGDGSILSLDLIDTQVHNVFENSSTAVTGQPYSVIYQPVNAANLSSQLAMLTW
                     RKEPLVGWGYRLSVTANRSIPTGIPIPASQKSYSVPANGVQQCSDGGSAVCIPYLKGY
                     AALDYTFADHTYAHLGVNYQGKNNTYFQPPFAIWDLTLRRPIQKSPMSVQATVYNLFN
                     TNNFGGLVTPNAGTPLIGENGAGQYGTYTQSVPFPMIPVQGRTLRVQLDYNVGGR"
BASE COUNT          225 a          398 c          358 g          203 t
ORIGIN      
        1 cgctcaaagc gggcgcgtac cagaactggt ggtatctcaa cggctgggcg ccggcggtga
       61 gcagctcgaa caagggcacc atcgttccgt tcacgcgtac cgtcggatcg ttcgatccgc
      121 atctggcgct cacgtatcag ccgcgcggcg gagaatcgtt ccgtctggca gccggtacgt
      181 cgacgacctt cccctacgcc ggtatcgtga gcggtaccgc gtacacgacg ccgggcgcaa
      241 ccggcaccgt cgcgctgaac ggcaagaacc cgctgctctc gccggagaaa gcgtacgagg
      301 ccgatctcgg tttcgacaag cgcatgggtg acggcagcat cctgagcctc gatctcatcg
      361 acacgcaggt gcacaacgtg ttcgagaact cctcgaccgc ggtgacgggc cagccgtaca
      421 gcgtgatcta tcagccggtc aacgcggcca atctctcgag ccagctcgcc atgctgacgt
      481 ggcgcaagga gccgctcgtg ggctggggct accgtctgtc ggtgaccgcg aaccgctcca
      541 tcccgaccgg cattccgatt ccggcttcgc agaagtcgta ctcggtgccg gccaacggcg
      601 tgcagcagtg cagcgacggc ggaagcgccg tctgcatccc gtacctcaag gggtacgcgg
      661 cgctggacta cacgttcgca gaccacacgt acgcgcatct cggcgtgaac tatcaaggca
      721 agaacaacac gtacttccag ccgccgttcg cgatctggga tctgacgctg cgccgtccga
      781 ttcagaaatc gccgatgtcg gttcaagcca cggtgtacaa cctgttcaac acgaacaact
      841 tcggcggact cgtgacgccg aacgccggaa cgccgctcat cggcgaaaac ggcgcgggcc
      901 aatacggcac gtacacgcaa tccgttccct tcccgatgat tccggtacag ggacgtacgc
      961 tgcgcgtgca gctcgactac aacgtcggcg gccgctaaac cgcatcgacg ctggtcaacg
     1021 agaaagcgct ccgggttacc cggagcgctt tctcatttct tcgacgtgct gcactttgga
     1081 ttcgagcgtt tgcggcacgt cgatgcgatc gtcgatcttc agcaggctgc cgacccgcgc
     1141 cgcaccccgc tcgtggcacc gctgatggat gacggcgagc gccc
//
LOCUS       DASUCJ010000961         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_56299, whole genome shotgun sequence.
ACCESSION   DASUCJ010000961 DASUCJ010000000
VERSION     DASUCJ010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56299"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..>1061)
                     /locus_tag="VFU90_11430"
     CDS             complement(99..>1061)
                     /locus_tag="VFU90_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480440.1"
                     /translation="GSDAVLLERYLRRPRHIEFQVLGDAHGNIIHLGERECSIQRRHQ
                     KIVEEAPSVALSPALRAEMGAAAVRAAKSVGYVNAGTCEFMLDEDGSYYFLEMNTRLQ
                     VEHPVTELAYGIDLVHWQLRIANGEQLTIRQEDVQPRGWAIETRIYAEDPANHMLPST
                     GTITEWDPPEGPGIRVDAGVQTGSEVSVFYDPMLAKLIVWGEDRHVAIERLTHALEDF
                     RICGIRANVPLLLWIARDEAYRQGDTTTRFLAERLDESIFSKPQEIPGEVLAAAASAL
                     LRNGRAPWRLGGVGVPLRLRIGDRDVILEATATAASPRRCRARS"
BASE COUNT          175 a          356 c          333 g          197 t
ORIGIN      
        1 gtcgcccggc gcctcgatgc ggtgttccat cttcatcgct tccagaacga taagcaggtc
       61 gtgcatgctg acggtgtcgc cgtcgcgcac ggcgatcttc acgatcttgc ccggcatcgg
      121 cgcggcgatg cggccgttgc cgtcgcctcc agtatgacgt cgcgatcgcc gatgcgcagc
      181 cgcagcggaa cgccgacgcc gcccaggcgc cacggcgcgc gtccgttgcg cagcagagcc
      241 gaagcggcgg cggcgagcac ctcgccgggg atctcctgcg gtttggaaaa gatcgactcg
      301 tccaaccgtt ctgcgagaaa acgcgtcgtc gtatcgccct gccggtacgc ttcgtcgcgc
      361 gcgatccaaa gcagcagcgg cacgtttgcg cgaataccgc agatgcgaaa atcttcgagt
      421 gcgtgcgtga gccgctcgat agcgacgtgc cggtcttcgc cccagacgat cagcttcgcc
      481 agcatcggat cgtaaaagac ggagacttcg cttcctgtct gcacgccggc gtccacgcga
      541 atgcccggac cctccggcgg atcccattcc gtaatcgtgc cggtcgaagg cagcatgtga
      601 ttggcgggat cttccgcgta gattcgcgtc tcgatcgccc agccgcgcgg ctgcacgtct
      661 tcctgccgga tcgtcagttg ctcaccgttc gcgatgcgca gctgccaatg cacgaggtcg
      721 atgccgtacg cgagctcggt gacgggatgc tccacctgca ggcgcgtgtt catttcgaga
      781 aagtagtagc tgccgtcttc gtcgagcatg aactcgcacg tgccggcgtt cacgtagccg
      841 accgattttg cagcacgcac ggctgccgca cccatctccg cgcgcagtgc gggcgatagc
      901 gcgaccgacg gcgcttcctc gacgatcttc tgatggcgcc gttgaatcga gcactcgcgc
      961 tcgccgagat gaatgatgtt gccgtgcgcg tcgcccagga cttgaaattc gatgtggcgc
     1021 ggccggcgca gataccgctc gagcagcacc gcatcgctgc c
//
LOCUS       DASUCJ010000962         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1202413, whole genome shotgun sequence.
ACCESSION   DASUCJ010000962 DASUCJ010000000
VERSION     DASUCJ010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202413"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFU90_11435"
     CDS             complement(<1..442)
                     /locus_tag="VFU90_11435"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5480441.1"
                     /translation="MHTDALDLQEEVAEALAYDPRITSGDVAVTVRDGIVTLRGTVPN
                     LNQKWEAEEIVKRVHGVRGVADELVVDLPAMHVRDDADIALAIERRFESSPIVPPGIT
                     FVVHDGRVTLAGSARWYYQSREAEYEVRRVAGVKDVANDVVVEPA"
     gene            556..3006
                     /gene="ppsA"
                     /locus_tag="VFU90_11440"
     CDS             556..3006
                     /gene="ppsA"
                     /locus_tag="VFU90_11440"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEU5480442.1"
                     /translation="MRSLEAIPAGADRLTETRFIRPFRSITNADVPAVGGKNASLGEM
                     YRALTPAGIEIPNGFAVTADAYREVVAPASGRLHEILDGIDPGDLRDFAERAQQAQAV
                     VAALPLPDELAAAVAGGYRELEAQYGANTSVAVRSSATAEDLPTASFAGAHESFLNVS
                     GERMVIEAYRACLASLFTERAIHYRIDQGFDHFKVALSVGVMKMVRSDMASSGVAFTL
                     DTETGFRDVVFITGAYGLGEKIVQGTVDPDEFYVHKPTYEAGFRAVLRRRLGAKASKL
                     LFAEGTQLRATTRSVGTPETERKRFCLTDDEVLELAGYCIKVEQHYSALAGRSMPMDV
                     EWAKDGVDGQIYLVQARPETAASQRTANVIDEYRLETNVRPAVTGRAVGSAAAAGPVR
                     VVRSNDDLARFQHGEVLVAETTTPDWEPVMKTAAAIVTDRGGRTCHAAIVARELGIPA
                     VVGTGYATTALQNVAEATVSCAQGEEGRVYAERIPFERLQVDVGELPAPPVKVMLTLG
                     DPGSAFARSALPNDGVGLVRIEFIVSDEIQVHPMALLHPERVHDAAASAKIRELVGSE
                     EPSEYFVRKLSEGVGTIAAAFYPKPVVVRMSDFKSNEYAQLLGGDAFEPVESNPMIGL
                     RGASRYTHPIYAEGFALECAAMKRVRETMGMRNVILMIPFCRRVGEAARTIDRMGELG
                     LRRGEDGLQVYVMCEIPNNVVLIDAFSQYFDGFSIGSNDLAQLTLGVDRDSELVAPEF
                     DERDPGVLEMLRQTVVGAHRNNRHVGICGQAPSDYPEVAQFLVSLGIDSISLNPDSVL
                     PTLRRFARSNEGAPLRSP"
BASE COUNT          528 a          987 c         1056 g          523 t
ORIGIN      
        1 cggccggttc gacgacgacg tcgttggcga cgtccttgac gccggcgacg cgccggactt
       61 cgtactccgc ttcgcgtgac tggtagtacc atcgcgcgct gccggcgagc gtaacgcggc
      121 cgtcgtgcac cacgaacgtg atgccgggag gcacgatcgg gctggattcg aagcgccgct
      181 cgattgcgag ggcgatatcc gcatcgtcgc ggacgtgcat cgccggcagg tcgacgacca
      241 gctcgtcggc aaccccgcgc acgccatgga cccgcttgac gatctcttcg gcttcccact
      301 tctggttgag attcgggacg gtgcctcgta aggtgacgat tccgtcgcgg acggtgacgg
      361 cgacgtcgcc cgaggtgatg cgcggatcgt aggccagtgc ctcggcgact tcttcctgga
      421 ggtcgagtgc gtcggtgtgc atgcggtgct cctctcgagc gttttgcgtc tcgccctcca
      481 tgctaactgc cgggtgtgaa ggcattgcga agccggcttc tcaaggtttt ccccggggca
      541 gcctaggcta ggggcatgcg aagcctcgaa gcgattccgg ccggcgccga ccggttaacg
      601 gaaacgcgat tcatccggcc ctttcgatcg ataaccaacg ccgacgtgcc ggcggtcggc
      661 ggtaagaacg cctcgctggg cgagatgtac cgagcgttga cgccggccgg gatcgagatt
      721 cccaacggat ttgccgtcac ggccgacgcg tatcgcgagg tcgtcgcgcc ggcgtccggc
      781 cggctccacg agatcctcga cggaatcgat ccgggcgacc ttcgcgattt cgccgaacgc
      841 gcgcagcagg cgcaggctgt ggtggccgcg ctgccgctgc cggacgagct cgcggccgca
      901 gtcgccgggg gatatcgcga actcgaagcg cagtatggtg caaatacgag cgtagcggta
      961 cggagctccg ctaccgcgga agatttaccg acggccagct ttgccggcgc tcacgagagc
     1021 ttcctcaacg tcagcggcga gcgcatggtg atcgaggcgt accgagcctg cctcgcaagc
     1081 ctctttaccg aacgcgcgat ccactaccgt atcgaccagg gattcgatca tttcaaggtc
     1141 gcgctgtcgg tcggcgtcat gaaaatggtg cgctcggaca tggcgtccag cggcgtggcg
     1201 ttcaccctcg acaccgaaac tgggttccgc gacgtcgtgt tcatcaccgg agcctacggc
     1261 ttgggcgaaa agatcgtgca gggaacggtc gatccggacg agttctacgt gcacaagccg
     1321 acctatgagg ccgggttccg cgcggtgctt cggcggcggc tcggcgcgaa ggcgagcaag
     1381 ctgctttttg ccgagggcac gcagctgcgg gccacgacgc gcagcgtcgg aacgccggag
     1441 accgagcgga agcgcttttg cctgaccgac gacgaggtgc tcgagctggc gggctactgc
     1501 atcaaagtcg aacagcacta cagcgcgctc gccggacgct cgatgccgat ggacgtcgag
     1561 tgggcgaaag atggcgtcga cggccaaatc tatttagtgc aggcgcggcc cgaaacggcg
     1621 gcgtcccagc gaacggctaa cgtcatcgac gaataccggc tcgagaccaa cgtccgcccg
     1681 gcggtcaccg gacgggccgt cggctccgca gcagcggccg gcccggtccg agtcgtccgc
     1741 agcaacgacg atctcgcgcg cttccagcat ggtgaggtgc tggttgccga aacgaccacg
     1801 cccgactggg agccggtgat gaaaactgcg gccgcgatcg tcaccgaccg cggcggccgc
     1861 acctgccacg ccgcgatcgt cgcgcgcgaa ctcgggatcc cggccgtggt aggaaccgga
     1921 tatgcgacga ccgcgctgca aaacgtcgcg gaggcgaccg tctcgtgcgc gcagggtgaa
     1981 gaagggcgcg tgtacgccga gcgcattccg ttcgaacggc tgcaagtgga cgtcggcgag
     2041 ctgccggcgc cgccggtgaa ggtcatgctg acgctcggcg atccgggaag tgccttcgcg
     2101 cgaagcgcgc tccccaacga cggggtcggc ttggtgcgca tcgagttcat cgtgtcggac
     2161 gagatccagg tgcacccgat ggcgctgctg catcccgagc gcgtgcacga cgcggcggcc
     2221 agcgccaaaa ttcgcgaact ggtcggcagc gaggagccca gcgagtactt cgttcgcaaa
     2281 ctgtccgaag gtgtcggcac cattgcggcc gcgttttatc cgaagccggt ggtcgtgcga
     2341 atgtcggatt tcaagagcaa cgagtacgcc cagctcctcg gaggcgacgc tttcgagccg
     2401 gtcgagagca acccgatgat cgggctgcgc ggcgcctcgc ggtacaccca tcccatctat
     2461 gcggaggggt tcgcgctgga gtgcgcggcg atgaaacgcg tgcgcgaaac gatgggtatg
     2521 cgcaacgtca tcctgatgat cccattctgc cggcgcgtcg gcgaagcggc ccgcacgatc
     2581 gaccggatgg gagaactcgg tttgcgtcgc ggcgaggacg gcctgcaggt gtacgtcatg
     2641 tgcgagatcc ccaacaacgt cgtcctgatc gacgcgttct cgcaatactt cgacgggttc
     2701 tcgatcggct cgaacgacct cgcgcagtta acgctcggcg tcgatcgcga ttcggaactg
     2761 gtcgcgccgg agttcgacga acgcgatccc ggcgtgctgg agatgctgcg tcaaacggtg
     2821 gtgggcgcgc accgcaacaa ccgccacgtc gggatttgcg gtcaggcgcc gtcggactac
     2881 ccggaggtcg cgcagttctt ggtaagcctc ggcatcgatt cgatcagcct caatcccgac
     2941 agcgtgctgc ccacgcttcg ccggtttgcc cgctccaacg aaggtgcgcc gctacgatcg
     3001 ccgtgagcga agtcccggtc gagatcgcgc cgggcgttgc cgcgcccgcg ttcttgggtc
     3061 ttccggaaaa ggcgcacggt atcgtgctgt tcgc
//
LOCUS       DASUCJ010000963         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_912002, whole genome shotgun sequence.
ACCESSION   DASUCJ010000963 DASUCJ010000000
VERSION     DASUCJ010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912002"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFU90_11445"
     CDS             <1..669
                     /locus_tag="VFU90_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480443.1"
                     /translation="VPTYKGQIDALGAAIATYGFLGYPLLQLCDIAIVRGEFVPVGRD
                     QQAHLEFGREVVRRFNHLYGDGAQILVEPQATLSEFPEVPGTDGRKMSKSYDNAILIA
                     DDEETTTKKVRSMITDPQKIRRGDPGRPEICPVFALWRFVNPLLLDGIAETCRSGALG
                     CVQDKTNFAEQLNAYLKPVRERYRTYRDDPAQVERIIEDGTARTRAVAASVLADVKHA
                     MRLT"
     gene            complement(694..894)
                     /locus_tag="VFU90_11450"
     CDS             complement(694..894)
                     /locus_tag="VFU90_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480444.1"
                     /translation="MTAGTIAVRVIAAIGLMLVVAALVYAVSNGRVFLFPFLLLLGFP
                     MAAVFRRRSGGPPGPPPRISLN"
     gene            complement(891..>1813)
                     /locus_tag="VFU90_11455"
     CDS             complement(891..>1813)
                     /locus_tag="VFU90_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5480445.1"
                     /translation="TPAYGIENVVVSPGAKPIIWNVLSALLNPGDDFVYFDPAYPAYA
                     SCSSYLQANVHAIPLLESRNWRMDLDELENRVSDKTKAIVINSPHNPTGGVLTRGDLE
                     RIAEMARRHDFIVIADEIYSRNFYLDSDYLSIAALDGMRDRTIVVDGFSKAYAMTGWR
                     LGYALMPAALAKTVTLFNNNTFSCVTAFVQMAGIAALTGPDEPVRRMNETFRQRRDRL
                     VSGLNDIPGMSCTLPEGAFYAFPNVTAITHDDRALAAFLLEEGGVACGGGSSFGAAGK
                     GYLRFSYAASLDDIDWALGQIATVLPKFKG"
BASE COUNT          360 a          577 c          591 g          285 t
ORIGIN      
        1 gttccgacgt acaaagggca gatcgacgcg ctcggcgccg cgatcgccac ctacggtttc
       61 ctgggttatc cgctgctgca gctgtgcgac atcgcaatcg tgcgcgggga attcgtcccg
      121 gtcggccgcg atcagcaagc ccatctcgaa ttcgggcgcg aggtcgtccg gcgtttcaac
      181 catctttacg gcgacggcgc gcagattctg gtcgagccgc aagcgacgct ttcggaattt
      241 cccgaagtgc cgggcaccga cggccggaag atgagcaagt cctacgacaa cgcgatcctg
      301 atcgcggacg acgaagaaac caccacgaaa aaagtacggt cgatgatcac cgacccgcaa
      361 aagatccggc gcggcgatcc ggggcgtccg gagatctgcc cggtgttcgc gttatggcgg
      421 ttcgtcaatc cgctgctgct cgacggcatc gcggagacgt gccgttccgg cgcactcggc
      481 tgcgtgcaag acaagacgaa tttcgccgaa cagctcaacg cctatctcaa gccggtgcgc
      541 gagcgctacc ggacgtaccg ggacgatccc gcccaggtgg aacgaatcat cgaagacggc
      601 accgcgcgca cgcgggcggt ggcggcgtcg gtgctggccg acgtaaagca cgcgatgcga
      661 ctgacctaac cgcggccagg gaacctccgt tccctaattg agcgagatgc gcggcggcgg
      721 tcccggcggc ccgccggacc ggcggcgaaa aaccgccgcc atcggaaaac ccagcagcaa
      781 taagaacggg aagaggaaaa cgcggccgtt cgagaccgcg tagacgagcg ccgccaccac
      841 gagcatcaac ccgatcgccg cgatcacccg cacggcgatc gttcccgcgg tcatcccttg
      901 aacttcggca ataccgtcgc gatctggccg agcgcccagt cgatgtcgtc gagcgaagcc
      961 gcgtaggaaa agcgcagata gccctttccg gcagcgccga aggacgagcc gccgccgcag
     1021 gcaaccccgc cttcttccag caggaacgcg gccagcgcgc ggtcgtcgtg cgtgatcgcg
     1081 gtgacgttgg gaaacgcgta aaacgcgccc tcgggcagcg tgcacgacat cccgggaatg
     1141 tcgttgagac cgctcaccag gcggtcgcgg cgttgacgga acgtctcgtt catccggcgc
     1201 accggctcgt cgggaccggt cagcgccgcg atgccggcca tctgaacgaa agccgtaacg
     1261 cagctgaaag tattgttgtt gaacagggtg accgtcttgg cgagcgccgc gggcatcagc
     1321 gcgtagccca accgccagcc ggtcatcgcg tacgccttcg aaaagccgtc gacgacgatc
     1381 gtgcggtcgc gcatgccgtc gagcgcggcg atcgacaagt agtcgctgtc gaggtagaaa
     1441 ttgcggctgt agatttcgtc ggcaatcacg atgaaatcgt ggcgccgcgc catctcggca
     1501 atccgctcga gatcgcctcg cgtcagcacg cccccggtcg gattgtgcgg cgagttgatg
     1561 acgatcgcct tggtcttgtc ggagacgcgg ttctcgagct cgtccagatc catgcgccag
     1621 ttgcgcgatt cgagcagcgg aatcgcgtgc acgttggcct gcagatagct cgagcaggat
     1681 gcgtacgccg gatacgccgg atcgaagtag acgaagtcgt cgccggggtt gagcagcgcc
     1741 gaaaggacgt tccagataat cggcttggca ccgggggaga cgacgacgtt ttcgatcccg
     1801 tacgcgggcg taa
//
LOCUS       DASUCJ010000964         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1049512, whole genome shotgun sequence.
ACCESSION   DASUCJ010000964 DASUCJ010000000
VERSION     DASUCJ010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049512"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFU90_11460"
     CDS             complement(<1..366)
                     /locus_tag="VFU90_11460"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEU5480446.1"
                     /translation="MDALVPPPAILPESSPMPLRPSALLGLLALVLFTVRVQGEPLNT
                     PPEDFVALFNGRDLEGWRGASTEDPRIWQKLAPEQQAEKLKAQLEDIHKHWTVEEGEL
                     VNDGKGLYLTTIKDYGDFEL"
     gene            complement(412..>1139)
                     /locus_tag="VFU90_11465"
     CDS             complement(412..>1139)
                     /locus_tag="VFU90_11465"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480447.1"
                     /translation="SLYGLNDPLYRGLLQMEADKVKNVIRAGHRLGWQMSSHVTGDAG
                     VDLVLDAVEAANADAPIAERRYTLIHAYFANSDTAARAAKLGVCVDTQPAWFYKDGDA
                     LLDAFGRKRIEPFIGAATWRAAGVKVALNSDHMQGFDPDRSLNPFNPFLAMYAAITRK
                     TESGQLIGPAERVSRLDALKMMTVDAAWLHFDERRKGTLEVGKLGDLAVLSDDFFRCD
                     EEQIPKLRSVLTVVGGKVVYRAD"
BASE COUNT          208 a          354 c          362 g          215 t
ORIGIN      
        1 caactcgaaa tcgccatagt ccttgatcgt cgtgaggtac agccctttgc cgtcgttgac
       61 gagttcgcct tcctcgacgg tccagtgctt gtggatgtct tcaagctgcg ctttgagctt
      121 ctcggcctgt tgctcgggag cgagcttctg ccagattctc ggatcctccg tcgaggcgcc
      181 gcgccaacct tccaggtcgc ggccgttgaa caacgccacg aaatcctcgg gcggggtatt
      241 gagcggctcg ccttgaacgc gaaccgtaaa cagaaccaag gcgagcaagc ccaacaaagc
      301 ggacgggcga agtggcatcg gggaagactc cggcaggatg gcgggcggcg ggacgagtgc
      361 gtccacttat catgggcgaa gattgagccc gaagcaagta attcgggcgg gctaatccgc
      421 ccggtagacg actttgccgc cgacgaccgt cagcaccgag cgcaatttgg ggatttgctc
      481 ttcgtcgcac ctaaaaaaat cgtcgctgag cacggccagg tcgcccagct tgccgacttc
      541 gagcgtccct ttgcgccgct cgtcgaagtg cagccaggcg gcgtcgacgg tcatcatctt
      601 caaggcgtcg agccgcgaga ctcgttcggc ggggccgatc aattggccgc tttcggtctt
      661 ccgcgtgatc gcggcgtaca tcgccaaaaa cggattgaac gggttgagcg agcgatcggg
      721 atcgaaccct tgcatgtggt cggaattgag cgcgaccttc acgcccgccg cgcgccaagt
      781 cgccgcgccg atgaacggct cgatccgttt gcggccgaac gcgtcgagta gggcgtcgcc
      841 gtctttgtag aaccaggccg gctgcgtatc gacgcacacg ccgagctttg ccgcccgagc
      901 ggccgtgtcg gagttggcaa agtaggcgtg gatcagcgta tagcgccgtt cggcgatcgg
      961 cgcatcggcg ttggccgctt cgacggcgtc gagcaccaga tcgacgccgg cgtcgcccgt
     1021 cacgtgcgaa ctcatctgcc agccgagacg gtgccccgcg cgaatcacgt tcttcacctt
     1081 gtcggcttcc atctgcaaca gcccgcgata gagcggatcg ttcagaccgt agaggctga
//
LOCUS       DASUCJ010000965         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1278858, whole genome shotgun sequence.
ACCESSION   DASUCJ010000965 DASUCJ010000000
VERSION     DASUCJ010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278858"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..742)
                     /locus_tag="VFU90_11470"
     CDS             complement(50..742)
                     /locus_tag="VFU90_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480448.1"
                     /translation="MLIGVTALAVIAPLVGGCPAPVRQPAVTPPAAWGTAFALPARLM
                     PRPVPADSLATPGAGLADDGGTACPTLADTTRPSARPRAITYSDAYYTRLTIHRIGSY
                     AMLPLFVTEYILGQKLINDSVPSSGLKTAHGLVAGGIAVVFATNTVTGVWNWMDSRRD
                     PSDHTLRNVHSAIMLASDLGILWTAASTPGGIRHTPADVRRQQARRHRAIAITSLSVS
                     TVGAAMMWIFKH"
     gene            1088..>1304
                     /locus_tag="VFU90_11475"
     CDS             1088..>1304
                     /locus_tag="VFU90_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480449.1"
                     /translation="MLRSRAVASFAVCAALSLASSSVAAQAPHYHVVKRMVLGHARAD
                     YIIVDPVGRRLYGLGDMVFDVDKDTVIG"
BASE COUNT          207 a          403 c          474 g          220 t
ORIGIN      
        1 ccacggctcg aaggcgtgga cgagttgctg catggggaac atcgccgcgc tagtgcttga
       61 agatccacat catcgcggcg ccgacggtgc tgacggaaag cgacgtgatc gcgatcgcgc
      121 ggtgccggcg tgcctgctgc cggcggacgt cggctggcgt gtgccggatg ccgccgggcg
      181 tggacgccgc cgtccagaga attccgaggt cggaagcgag catgatcgcc gagtgaacgt
      241 tgcgcagcgt gtgatcgctg ggatcgcggc gcgagtccat ccagttccat acaccggtca
      301 cggtattggt cgcgaacacg acggcgatgc cgccggcgac gagcccgtgc gcggtcttga
      361 ggccggacga cggtacgctg tcgttgatga gcttctggcc gaggatgtac tcggtgacga
      421 aaagcggcag cattgcgtag ctgccgatac ggtggatcgt gagtctcgtg tagtaggcgt
      481 cgctgtaggt gatggcccgc ggtcgcgccg atgggcgcgt cgtgtcggcc aacgttgggc
      541 aggccgtccc gccgtcatcc gcgagaccgg cgccgggcgt ggcgagcgag tcggccggaa
      601 cagggcgggg catgaggcgc gccgggagcg caaacgctgt gccccacgcc gccggcggcg
      661 tgacggccgg ctgcctaacg ggcgctggac atccgccgac caggggcgcg atgacggcga
      721 gcgcggtgac gccgatgagc atggttccgt gttgcccggg gaatgccgca tccactgcac
      781 gcggcgccga cgccgatcga ctcggcgcac ctcttatacc tagccgacgt gcacgtcgcg
      841 gcaacaaaag atcttcacgt ggaggtcatt caagcgaaca cagcggcgct gcctacgtca
      901 gccctctccg gagccaggcg ctggcgccga tgaccagccc agcgcggcgg atcgcgattg
      961 cgtgtcacgt ggctgacagg taagcggtat acgttgattc tgcttacgac gtacggcacg
     1021 gcgcgcggag cggtgcatcg ctgctcggtc gcgcttgtca tcgcacccct tcatccgagg
     1081 acacgacatg cttcggtccc gcgcggtcgc atcgttcgcc gtatgtgcgg cgttgtcgct
     1141 tgcgagctca tcggttgcgg cgcaggcgcc gcactatcac gtggtcaagc gcatggtgct
     1201 gggtcacgcg cgcgccgact acatcatcgt cgatcccgtt gggcggaggc tctacgggtt
     1261 gggcgacatg gtgttcgacg tggacaagga caccgtgatc ggca
//
LOCUS       DASUCJ010000966         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_25581, whole genome shotgun sequence.
ACCESSION   DASUCJ010000966 DASUCJ010000000
VERSION     DASUCJ010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25581"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFU90_11480"
     CDS             complement(<1..412)
                     /locus_tag="VFU90_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480450.1"
                     /translation="MKAIDRKLLRDLAQMRGQVLTIALVVACAVAGFVGTLSTYVSLD
                     HARQQFYEQARFGHVFSSLKRAPNEYAARLAEIPGVADVQTTIVFDATLDLPGVQEPL
                     VGRMVGVPAGGVRRLDRIYLRHGRMLDPTHPHEAI"
     gene            complement(409..1125)
                     /locus_tag="VFU90_11485"
     CDS             complement(409..1125)
                     /locus_tag="VFU90_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480451.1"
                     /translation="MQAQHQTVFVAHGLTKVYRMGDVEVRALNGVDLEIYAGEFIVLL
                     GPSGSGKSTLLNILGGLDTPSAGSAFWRDHDLTKADDDALTRYRRDHVGFVFQFYNLI
                     PSLTVRENVALVTDIVPNPMRPEEALALVDLSDRLNHFPAQLSGGEQQRVAIARAIVK
                     RPEVLLCDEPTGALDYATGKRVLDVIARVNNELGTTAIVITHNAAIAGMADRVLYLAD
                     GRIARVQTNPRRLTSDEVHW"
BASE COUNT          215 a          366 c          386 g          207 t
ORIGIN      
        1 cgatcgcttc atgcggatgt gtcggatcga gcatgcgccc gtgccgcaag tagatccgat
       61 ccaaacggcg aacgccgccc gcaggcacac cgaccatacg gccgaccaga ggctcttgca
      121 cgccgggaag atcgagcgtg gcgtcgaaga cgatcgtcgt ctgcacgtcg gccacgccgg
      181 gtatctcagc cagacgcgcc gcgtactcgt tcggtgcgcg cttgagggaa ctgaagacgt
      241 gcccgaagcg cgcctgttcg taaaattgct ggcgcgcgtg gtcgagcgac acgtaagtcg
      301 acagcgtgcc gacgaagcct gcgacagcgc acgcgacgac gagtgcgatc gtcagcacct
      361 ggccccgcat ctgcgccaga tcgcgtaaca gcttgcggtc gatcgctttc accagtgcac
      421 ctcgtccgac gtgagccggc gcgggttcgt ctgcacgcgc gcaatgcgtc cgtcggccag
      481 ataaagtacg cggtcggcca tgccggcgat tgcggcattg tgcgtgataa cgatggcggt
      541 ggttccaagc tcgttgttga cgcgcgcgat gacatcgagc acgcgcttgc ccgtcgcgta
      601 gtcgagcgcc cccgtcggct catcgcagag cagcacctcc ggccgtttca cgatcgcccg
      661 cgcgatcgcc acgcgctgtt gttcgccgcc ggagagctgc gccgggaagt gattcaggcg
      721 atcgctgagg tccacgagcg cgagcgcctc ttcgggccgc atgggattgg gcacgatgtc
      781 cgtgacgagc gcgacgtttt cccggacggt gaggctggga atcaggttgt aaaactggaa
      841 gacgaagccg acgtgatcgc ggcgatagcg cgtgagcgcg tcgtcgtcgg ccttggtcaa
      901 atcgtgatcg cgccagaaag cgctgcctgc gctgggtgta tcaagaccgc cgagaatatt
      961 gagcagcgtt gacttgccgc tgccggaggg acccagcaaa acgatgaatt cgccggcata
     1021 gatctcgagg tcgacgccgt tcagcgcgcg cacctcgaca tcgcccatgc gatatacctt
     1081 ggtgagtccg tgcgcgacga acacggtttg atgttgcgcc tgcatcagca cgaacctcgg
     1141 tccgaatgct tagtatggcc cgatgacgcc tggc
//
LOCUS       DASUCJ010000967         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1171885, whole genome shotgun sequence.
ACCESSION   DASUCJ010000967 DASUCJ010000000
VERSION     DASUCJ010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171885"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFU90_11490"
     CDS             complement(<1..347)
                     /locus_tag="VFU90_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5480452.1"
                     /translation="MGDPQLVRGYDVNSINSNECVSSTTSSCPVFDRLIGSKMAVANV
                     ELRIPVLGSDQFGLINFPYLATELSPFVDAGVAWTSANPPNIEFSTTSQGRVPIVSAG
                     VSMRVNVLGYLVA"
     gene            complement(360..>1292)
                     /locus_tag="VFU90_11495"
     CDS             complement(360..>1292)
                     /locus_tag="VFU90_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480453.1"
                     /translation="YSLHTIQPDEAGGTLLGPNVDTTSIAGNMPPLKPVEVGEITERV
                     HDPVTGLPPAEQYATLPYNPKLSLEYLGSPGVGVGVGPYGTIAGGGVTAYFADQLSNN
                     VIGVELGGAGDIQDFGGSVFYANLAHRWNWQVGLSHTVYPLIGADAFDTTLTDNGGNQ
                     VPATVFEQRLIRQYYDQVNAAAQYPLDLTKRFEFGISATHLSYGTQLFRLFIDPQTGI
                     PFGETQQNVSSGLPTWNYVSLTAAFVGDYSYYGFTSPIAGGRYRFEVDPSFGSLRMEN
                     VLADYRRYFFMRPITFAFRAIHLGRYGPDADNPE"
BASE COUNT          235 a          408 c          435 g          214 t
ORIGIN      
        1 tccgccacga gatagcccaa cacgttcacg cgcatgctca cgcccgcgct gacaatcggc
       61 acgcggccct gcgacgtggt ggagaactcg atgttcggcg ggttggccga cgtccacgcc
      121 acgccggcgt ccacgaacgg cgagagctcg gtggccaggt acgggaagtt gatcagcccg
      181 aactggtccg agcctaacac aggaatacgg agctcgacgt tcgccaccgc catcttgctg
      241 ccgatcaggc ggtcgaacac cgggcagctg ctcgtggtcg acgagacgca ctcgttcgag
      301 ttgatcgagt tcacgtcgta gccgcgcacc agctgcgggt cgcccacgta cagcggaaac
      361 tactcggggt tgtcggcgtc cggcccgtag cggccgaggt gaatggcgcg gaatgcgaag
      421 gtgatcggac gcatgaagaa gtagcgccgg tagtccgcca gcacgttctc catgcgcagc
      481 gatccgaacg acggatcgac ctcgaagcga tagcggccgc cggcgatcgg cgacgtgaag
      541 ccgtagtacg agtagtcgcc cacgaacgcc gccgtcagcg acacgtagtt ccacgtcggc
      601 aaccccgacg acacgttctg ctgcgtctcg ccgaacggaa tgccggtttg cgggtcgatg
      661 aacagccgga agagctgcgt gccgtagctc aggtgcgtcg cggagatgcc gaattcgaag
      721 cgcttggtga gatcgagcgg atattgcgcg gccgcattca cctgatcgta atactgccga
      781 atgagccgct gctcgaatac ggtggccggc acctggttgc cgccgttatc cgtcagcgtg
      841 gtgtcgaagg catcggcgcc gatgagcgga tacacggtgt gcgagagtcc cacctgccag
      901 ttccaccggt gggcgagatt ggcgtagaag accgatccgc cgaagtcctg gatgtcgccg
      961 gcaccgccta actcgacacc gatgacgttg ttgctcaact ggtcggcgaa gtacgcggtg
     1021 acgccgccgc cggcgatcgt tccgtaaggc ccgacgccga cgccgacgcc cggcgagccc
     1081 agatactcga gcgacagctt cgggttgtac ggcagcgtcg catactgctc ggccggcggc
     1141 aggccggtga ccggatcgtg cacgcgctcg gtgatctcgc cgacttccac cggcttgagc
     1201 ggcggcatgt tgccggcgat cgacgtcgtg tccacgttag gcccgagcag cgtgccgccg
     1261 gcttcgtcgg gctggatggt gtgcagcgaa ta
//
LOCUS       DASUCJ010000968         1467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_636862, whole genome shotgun sequence.
ACCESSION   DASUCJ010000968 DASUCJ010000000
VERSION     DASUCJ010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636862"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1467)
                     /locus_tag="VFU90_11500"
     CDS             complement(93..>1467)
                     /locus_tag="VFU90_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU5480454.1"
                     /translation="PSTYNPRIEEHHLTFARAEHVSNGRRVITWAAQDVPKPEPEPLA
                     PDSSYGESITLAAPRTWSDVAHWYAGLSRDRYATTPAIDSKIAEVVKDARTLDDSIRA
                     VHRWVAQDFRYVSLSLGIGGYQPHQPESMFENKYGDCKDKATFFIAAMRRLGVTAYPV
                     LLSGDGGVDRALPSAHQFDHMIAAVKRPDGSYTYVDLTADLAPYGALPPGEPGEFGLV
                     VHPDGTSEEVTFPSDSAARNLSRVDIDGALAADGLFTGKWIRTATGLQQYGLRSSMSR
                     STQMDSTQRARATLAIANAIIQGATGDSLQVFDGRDLIAKPRISVLLSNGKMVNDAGT
                     TKILTLPIRDFAVPNVVAGLEQRGPRVNPIDIEKVWGPHEELEDFRVALPAGWKAQLP
                     KSVTETSVFGTYTARYTQAGDTLQVTRSLVGAAGVQPASAMPALIAWLKAMSADDVKY
                     IVINAGS"
BASE COUNT          239 a          529 c          448 g          251 t
ORIGIN      
        1 ggcggtcgat cgaccgcgcg cgaacaccgt acacaggtcc cacgctcgtg ccgtcatcgg
       61 actcacggca cgaggtacca attattgcgg gctcaactcc cggcgttgat cacaatgtac
      121 ttcacatcgt ccgccgacat cgccttgagc cacgcgatga gcgccggcat cgcgctcgcc
      181 ggctgcacgc cggcggcgcc cacgagcgac cgtgtcacct gcagcgtgtc gcccgcttgt
      241 gtgtatcgcg ccgtgtacgt accgaacaca ctcgtttcgg tcaccgactt gggcagctgc
      301 gccttccaac cggccggcag cgcaacccgg aagtcctcga gctcctcgtg gggtccccac
      361 actttctcga tgtcgatcgg attcacgcgc ggcccgcgct gctcgagccc cgccaccacg
      421 ttaggcacgg cgaagtcgcg gatcgggagc gtgaggattt tcgtcgtgcc ggcatcgttc
      481 accatcttgc cgttggacag gagcaccgaa atgcgcggct tggcgatcag atctcgcccg
      541 tcgaaaacct gcaggctgtc gcccgtcgcg ccctgaatga tcgcattcgc gatggcgagc
      601 gtcgcccgcg cgcgttgcgt cgagtccatc tgcgtcgagc gtgacatcga gctccgcaat
      661 ccatactgct gcaagcccgt cgccgtccgg atccacttcc ccgtgaacaa gccatccgcg
      721 gccaacgcgc cgtcgatgtc cacgcgcgac aggttgcgcg cggccgaatc cgacgggaac
      781 gtgacctcct cgctcgtgcc atccggatgc accacgaggc cgaattcacc cggctcgccc
      841 ggcgggagcg caccgtacgg cgcgagatcg gccgtcaggt cgacgtaggt gtacgagccg
      901 tcgggccgct tcaccgccgc gatcatgtga tcgaactggt gcgcgctcgg cagcgcgcgg
      961 tccacgccgc cgtcaccgct caacagcaca ggatacgccg tcacgcctaa ccggcgcatc
     1021 gccgcgatga agaaggtcgc cttgtccttg cagtcgccgt acttgttctc gaacatcgac
     1081 tccggctggt gcggctgata gccgcctatg cctaacgaaa gcgacacgta ccggaagtcc
     1141 tgcgccaccc accggtgcac cgcgcggatc gagtcgtcca gcgtcctcgc gtccttcacc
     1201 acctcggcaa tcttcgagtc gatggccggc gtcgtcgcat accgatcgcg cgagagaccg
     1261 gcgtaccagt gcgccacgtc actccatgtc cgcggcgccg cgagcgtgat cgattcgccg
     1321 tacgacgagt cgggcgccag cggctccggc tccggcttcg gaacgtcctg cgccgcccac
     1381 gtgatcacgc gccggccgtt cgacacatgc tcggcgcgcg caaacgtcag gtggtgctcc
     1441 tcgatgcgcg gattgtaggt ggatgga
//
LOCUS       DASUCJ010000969         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_743947, whole genome shotgun sequence.
ACCESSION   DASUCJ010000969 DASUCJ010000000
VERSION     DASUCJ010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743947"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>1090)
                     /locus_tag="VFU90_11505"
     CDS             complement(109..>1090)
                     /locus_tag="VFU90_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480455.1"
                     /translation="APDRSALPSTAPSLAGAATVTPVQPPRVPRARPAWFRAAPDSLR
                     KGVYVAEYYTNAILGYQWATRENRPPICSIPATFVVNVTTDPLGNLVDPDGGARSVNV
                     FRGPNMCGPQVGSFADNDGQPSDAATTDAATGTIYVANLQAAGQAYGNVSVCALAGGC
                     TRKRGNKAIGGQLFAVAEDQHGNVYASGYANASGSGASLVVWKGGQGSGARLKAYRNG
                     SPGGLDVDKQGRLLALDTFANGNGALYVYTGCPTHCTAHGPFALKGESVFGKVNAGGT
                     LFEAADFEYGQIDVYSYAGTKGITYLYSFSDGLTPSGDVEGIALSPAAFD"
BASE COUNT          179 a          387 c          335 g          189 t
ORIGIN      
        1 gagcaacctg ccagaaatgc ggcgaccgcg gccgccgcaa ccgctgggcg cgcaccgcgc
       61 cctacaacca aacgcatact ctgaaaaact ccttccaaga aaaaagcgtt agtcgaatgc
      121 cgccggactc agcgcgattc cttccacgtc gccgctcggc gtcaaaccgt ccgagaaact
      181 atagaggtac gtgatgcctt tcgtgccggc gtaactatac acgtcgatct gtccgtattc
      241 gaaatcggcc gcttcgaaca gcgtcccgcc cgcgttgact ttgccgaaca ccgactcacc
      301 tttcaatgca aacggtccgt gcgcggtgca gtgcgttgga cagccggtat agacgtacag
      361 cgcaccgttg ccgttggcga acgtgtcgag cgcgagcagc cgcccttgct tgtcgacgtc
      421 cagcccgccc ggcgaaccgt tgcggtacgc tttcagccgc gcgccgctgc cttgtccgcc
      481 cttccacacc actagcgacg caccgctgcc cgatgcgttg gcgtaccccg acgcgtacac
      541 gttgccgtgt tggtcctcgg caactgcgaa cagttgtccg ccgatcgctt tgttcccgcg
      601 cttacgcgtg cagccgcccg cgagcgcgca caccgacacg ttgccgtacg cctgtcccgc
      661 cgcttgaagg ttggcgacgt agatcgtccc cgtggccgca tcggtcgtcg ccgcgtccga
      721 cggctggccg tcgttgtcgg cgaagctccc cacttgcggc ccgcacatgt tcggtccgcg
      781 gaacacgttg acgctgcgcg cgccgccgtc cggatcgacg aggtttccga gcggatcggt
      841 cgtgacgttc accacgaacg tcgccggaat cgaacagatc ggcggccggt tttcgcgcgt
      901 cgcccactgg tagccgagaa ttgcgttggt gtaatactcc gccacgtaga cgcctttgcg
      961 cagcgaatcc ggcgccgctc ggaaccacgc cggacgggcg cgcggcacgc gcgggggctg
     1021 caccggcgtg acggtcgccg ctccggcgag cgacggagcg gtcgacggca gcgcgctgcg
     1081 atccggcgcg
//
LOCUS       DASUCJ010000970         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_697806, whole genome shotgun sequence.
ACCESSION   DASUCJ010000970 DASUCJ010000000
VERSION     DASUCJ010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697806"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFU90_11510"
     CDS             <1..896
                     /locus_tag="VFU90_11510"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU5480456.1"
                     /translation="ERRPSDAGGGDVLKPLGEALGGWEPQKRAHREREPHELLAAAWS
                     EIVGEEVARNSRPLRIAGDALVIVTRSSAWSQQLSFFSEHILEAARARLPQTPIAAVR
                     FRVGKVAGAASQAVPASRPVRSERPPPRPAAATADEAIARFRSDVDERRRAKRSAGWK
                     ECRGCGALIAPRGHALCVTCANARAQQREAAAVRLLFEAPWLGFAGTAELIDGLTRRE
                     YESIRARLLARWWERLARALAAKRLSRDGRERLIASSYVVLKSKLPPEEIEPATVRNV
                     LGDELHDLIYGTERQTKTNVE"
     gene            886..2784
                     /gene="gyrB"
                     /locus_tag="VFU90_11515"
     CDS             886..2784
                     /gene="gyrB"
                     /locus_tag="VFU90_11515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5480457.1"
                     /translation="MSSNYTGEQIEVLRGLEAVRKRPGMYIGNTAERGLHQLVYEAVD
                     NAVDEALAGYAKNVRVTLYKDGSCSVQDDGRGIPVDIHAEEKLPAVEVVMTMLHAGGK
                     FGKGGYKVSGGLHGVGISVVNALSEWMTTRVSRDGWLWEMKFVRGITVQKLKKISKTD
                     GSGTLQWFKPDTTMFEVVDFHWDILQKRLRELAFLNRGLSITLRDERGEEPRERNYKF
                     DGGIVSFVEWLNEKKDPLHPIISTHAERDGVDVEVAMQYTDTYADQIFAYANNINTIE
                     GGMHLLGFRTAVTNAVNSYARKRGALKESEPSLSTDDIMEGLTAVVSVKLENPQFEGQ
                     TKTKLGNNKVRSIVYGLVNERLDFFFEENPKQARTIVEKCMQAQRAREAAKKARDLSR
                     RKNALEGSGLPGKLVDCKNTNPAESEIFLVEGDSAGGTAKGGRDPNTQAILPLRGKIL
                     NVEKARLDKVLANEEIRTMITAFGTGFGDEFNVAKLRYHKIIIMTDADVDGSHIRTLL
                     LTFFYRQMRPLVDGGHVYIAQPPLYGIRKGKKQWWAFNEAEYRAVIGDADPKDFVVQQ
                     YKGLGEMDAEQLAETTMEVGHRRLEQITIEDAVEAEQIFTDLMGDKVEPRKQYIFDYA
                     KSVKTLDL"
     gene            2889..>5039
                     /locus_tag="VFU90_11520"
     CDS             2889..>5039
                     /locus_tag="VFU90_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480458.1"
                     /translation="MRKRLLLFGLAALVTVVGLAIARRHDVVRFVIERAAGLASGYGV
                     SIGDQRIGTNQAALYDVRVSRDGVPLLHAGRIDVRYSLRDLLPGSTHRFGLVALDVGH
                     AAVTIVRFRDGTYNFIVPAPPGGLPPPPQPPHVDAVPIRFALRMHDASLELREPGAYD
                     PSARVIGIGNFNVDASIDTATRTHYSARGAFLAERPAPFTIVGTIDAVRGYAMHRARA
                     PRFPLRALANYFADTPIVRIVKGGARNFDARLFAFDVQPNVAPAYHANLQLDVDGGAL
                     RLSALDAPVESVRGRLQLVDNVVFLRHIHAALAGIPLHLAGGIYDLSGDLTGGAKLRI
                     GIYGTGDLGQLRRAFAFTRKEPISGVVRLGVLVEGPLDNPIIVARAKADRAVYRLLPF
                     DSLDAGVVYRDNVVALLPLHAYYGGTEVGIRGTMGIGKHLQSELALHIVGSADRLPYL
                     DEMLDRESMLVDAVAAGTDSDFRVRGAAASARGVSRVAALIAMEPNGTAAVEPFWLHT
                     QRGDFDGGYLLDRPHDTSAFWGLAGNLQMRAPTYKAFPGLSLPAIPNVNGRIASVAIA
                     GGGAGRNVVLAGLVSGDRADIAGVKFDRVAAAFAGTLTNAQIGLLHASGPWGEFDGNG
                     AFSSRVFVAGGSYRGTLDGLQPFLGSAIPGHGPISGHTAIAVEGNRILVQGSNLAMPG
                     ATLRGVPVSHASITVGVEPDRLRVYSAHARAAGGD"
BASE COUNT          896 a         1655 c         1705 g          783 t
ORIGIN      
        1 gtgagcggcg cccgagtgac gccggcggag gcgacgtgct gaagccgctg ggggaagctc
       61 tgggcggctg ggagccccaa aaacgcgcgc accgcgagcg cgagccgcac gagcttttgg
      121 ccgcggcgtg gtcggagatc gtcggcgagg aggtggcgcg caactcccgt ccgctgcgga
      181 tcgcgggcga cgcactcgtc atcgtcaccc ggtccagcgc gtggagccag cagctcagtt
      241 tcttttccga gcacatcctg gaagcggcgc gcgcccgcct gccgcaaacg ccgattgccg
      301 ccgtacgatt tcgggtcggg aaggtcgccg gcgccgcatc ccaagcggtg cccgcatccc
      361 gtcccgtccg gtccgaacgg ccgccgccgc gcccggcggc cgcaaccgcg gacgaagcga
      421 tcgcgcggtt ccgaagcgac gtcgacgaac ggcggcgggc aaaacgcagc gccgggtgga
      481 aggagtgccg ggggtgcgga gccttgatcg cgcctcgcgg ccacgcgctg tgcgtcacgt
      541 gcgcaaacgc gcgcgcgcag cagcgtgaag ccgcagcggt acggctgctt ttcgaggcac
      601 cctggctggg cttcgccgga acggcggagc tgatcgacgg gctcacccga cgagaatacg
      661 agtcgattcg agcgcggctg ttggcgcgct ggtgggagag gcttgcgcga gcgctcgccg
      721 ccaaacgcct ttcgcgcgac ggacgcgagc ggcttatcgc gagctcgtac gtcgtgctca
      781 agagcaaact gccgcccgag gagatcgaac cggcgaccgt gcgcaacgtg ctcggtgacg
      841 agctgcacga cttgatttac ggaacggaac gtcaaacaaa aacgaatgtc gagtaactac
      901 acaggcgaac agatcgaggt tcttcgcggg ctcgaagcgg tgcgaaagcg gccgggcatg
      961 tatatcggca acaccgccga gcgcgggctg caccagctcg tgtacgaagc cgtcgacaac
     1021 gcggtcgacg aagcgctggc gggctacgcc aaaaacgtcc gggtgacgct ctacaaggac
     1081 ggctcctgtt ccgtgcagga cgacggccgc gggattccgg tcgacattca cgccgaagaa
     1141 aaactgccgg cagtcgaagt ggtcatgacg atgctgcacg ccggcggaaa attcggcaag
     1201 ggcggctaca aggtgtcggg cggcctgcac ggcgtgggca tctcggtggt gaacgccctc
     1261 tcggagtgga tgacgacgcg cgtcagccgc gacggctggc tgtgggagat gaagttcgtg
     1321 cgcggcatca ccgtgcaaaa gctcaagaag atctccaaga ccgacggcag cggcacgctg
     1381 cagtggttca agcccgatac cacgatgttc gaggtcgtcg atttccattg ggacattctg
     1441 cagaagcgct tgcgcgagct ggccttcctc aaccgcggcc tctcgatcac gctgcgcgac
     1501 gagcgcggcg aggagccgcg cgagcgcaat tacaagttcg acggcggcat cgtgtcgttc
     1561 gtggagtggc tcaacgaaaa gaaagacccg ctgcacccga tcatctcgac gcacgccgaa
     1621 cgcgacgggg tcgacgtcga agtggcgatg cagtataccg acacgtacgc cgatcagatc
     1681 ttcgcctacg ccaacaatat caacacgatc gaaggcggga tgcacttgct cggcttccgg
     1741 acggcggtca cgaacgcggt caactcgtac gcgcgcaagc gcggggcgct caaagagtcg
     1801 gagccgtcgc tctcgaccga cgacatcatg gaagggctga ccgcggtcgt ttcggtgaag
     1861 ctcgagaacc cgcagttcga aggtcagacc aagaccaagc tcggcaacaa caaggtgcgc
     1921 agcatcgtct acggcctggt caacgaacgg ctggactttt tcttcgaaga gaaccccaag
     1981 caggcgcgca cgatcgtcga gaagtgcatg caggcgcagc gcgcccgcga agccgccaag
     2041 aaagcgcgcg atctctcgcg ccgcaagaac gcgctcgagg gctcgggcct gcccgggaag
     2101 ctcgtcgact gcaagaacac caatccggcc gaaagcgaga tctttctcgt cgagggcgat
     2161 tcggccggcg gcacggctaa aggcggccgc gaccccaaca cgcaggcgat cctgccgctg
     2221 cgcggcaaga tcctcaacgt cgagaaagcg cgcctcgaca aggtcttggc caacgaagaa
     2281 atccgcacga tgatcaccgc gttcggcacc gggttcggcg acgagttcaa cgtcgccaag
     2341 ctgcgctatc acaagatcat catcatgacc gatgcggacg tggacggctc gcacatccga
     2401 acgcttctgc tgacgttctt ctaccggcag atgcggccgc tggtggacgg ggggcacgtg
     2461 tacatcgcgc agcccccgct ctacggcatt cgcaagggaa agaagcagtg gtgggcgttc
     2521 aacgaagccg agtaccgtgc cgtgatcggc gacgccgatc ccaaagactt cgtcgttcag
     2581 cagtacaagg gactcggtga gatggatgcc gagcagctcg ccgagacgac gatggaagtc
     2641 ggacaccggc ggctggagca gatcacgatc gaagacgccg tcgaggcgga gcagattttt
     2701 acggacttga tgggcgacaa agtcgagccg cgcaagcagt acatcttcga ttacgcaaag
     2761 agcgtcaaga ccctagacct gtaagggacg ccgatcggcc ggcggctcgc gcttcgcggg
     2821 cgttcatcga atcgtaaggt ccgcctgcag gggcggcctc gccgcaccgc ggtataacaa
     2881 cctcgtacgt gcgcaaacgc ttgctgctgt tcggcctggc ggccctcgtc accgtagtcg
     2941 gcctcgcgat cgcgcgacgg cacgacgtcg tgcgtttcgt catcgagcgc gccgccggac
     3001 tcgcgagcgg atacggggtg agtatcggcg atcagcgcat cggaaccaac caagccgcgc
     3061 tgtacgacgt gcgcgtgtcc cgggacggcg tgccgctgct gcacgccggc cgcatcgacg
     3121 tccgctattc gctgcgcgac ctgctgccgg gaagtacgca ccggttcgga ctcgtcgccc
     3181 tcgacgtcgg ccacgcggcg gtaacgatcg tgcgtttccg cgacgggacg tataatttca
     3241 tcgtgcccgc cccgcccggc ggcctgcccc cgccgccgca gccgccccac gtcgacgcgg
     3301 taccgatccg cttcgcgctg cgcatgcacg acgcgtcgct cgaattgcgc gaacccggcg
     3361 cctacgatcc cagcgcgcgc gtcatcggta tcgggaactt caacgtggac gcctcgatcg
     3421 acacggcgac ccgcacgcac tactccgccc ggggcgcgtt cctcgccgag cggccggcac
     3481 cgtttacgat cgtcggaacg atcgacgccg tccgcggcta cgcgatgcat cgggcgcgcg
     3541 cgccgcgctt cccgctgcgc gcgctggcca attactttgc ggacacgccg atcgtgcgga
     3601 tcgtcaaagg cggcgcgcga aacttcgacg cgcggctttt tgcattcgac gtgcagccga
     3661 acgtcgcacc ggcctatcac gccaacctcc agctcgacgt cgacggcggc gcgctgcggc
     3721 tgtccgcgct cgacgcaccc gtggaatcgg ttcgcgggag gctgcagctg gtggacaacg
     3781 tcgtcttcct gcggcacatt cacgccgcgc tggccggcat accgctgcac ctggccggcg
     3841 gcatttacga tttgagcggc gatctgaccg gcggcgccaa gttgcgcatc ggcatttacg
     3901 gtacgggaga tctcgggcag ctgcgccggg cgttcgcgtt cacgcgcaaa gagccgatct
     3961 ccggggtggt ccggctgggc gtcttggtgg aaggtccgct cgacaacccg atcatcgtcg
     4021 cccgcgcgaa ggccgatcgc gcggtctacc ggttgctgcc gttcgattcg ctcgacgccg
     4081 gcgtcgtcta tcgcgacaac gtcgtcgcgc tgttgccgct gcacgcttat tacggcggta
     4141 ccgaagtcgg catccgcggc acgatgggaa tcggcaagca cttgcaatcg gagctcgcgc
     4201 tgcacatcgt cggctcggcg gaccgcttgc cgtacctcga cgagatgctc gaccgcgagt
     4261 cgatgctggt cgacgccgtc gccgccggaa ccgattcgga ctttcgcgtg cgcggtgcgg
     4321 ctgcgtcggc ccgcggagtt tcgcgcgtgg cggcgctcat cgcgatggag ccgaacggca
     4381 ccgcggcggt cgagccgttc tggctgcaca cgcagcgcgg cgatttcgac ggcggttatc
     4441 tgctcgaccg gccgcacgat acgagcgcgt tctggggttt ggcggggaac ctgcagatgc
     4501 gcgcgcccac ctacaaggcg ttcccggggc tctccctacc cgcaattcca aacgtcaacg
     4561 gaagaatcgc gagcgtcgcc atcgcgggcg gaggcgcggg aaggaacgtc gtcctggcgg
     4621 gcctcgtgtc gggcgatcgc gccgacatcg ccggcgtgaa gttcgaccgc gtcgccgccg
     4681 cgtttgcagg cacgctgacc aacgcccaaa tcggcttgct gcacgcctcg ggtccgtggg
     4741 gagagttcga cggcaacggg gcgttctcgt cgagggtgtt cgtcgccggc ggaagctatc
     4801 gcgggacgct cgatgggctg cagccgttcc tcggcagcgc gatcccgggg cacggtccga
     4861 tctcgggcca tacggcgatc gcggtcgaag gaaacaggat cctcgtgcaa ggttcgaatc
     4921 tcgcgatgcc gggtgcgacg ctgcgcggcg tgccggtctc gcatgccagc atcacggtcg
     4981 gcgtcgaacc cgatcgcctg cgcgtctatt ccgcccacgc ccgggcggcc ggcggcgac
//
LOCUS       DASUCJ010000971         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1324715, whole genome shotgun sequence.
ACCESSION   DASUCJ010000971 DASUCJ010000000
VERSION     DASUCJ010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324715"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFU90_11525"
     CDS             <1..207
                     /locus_tag="VFU90_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5480459.1"
                     /translation="AEGIFVGSGIFKSADPAKFAKAIVDATTHFNDPKVVTDACRSLG
                     GSDAMAGLDLRTLDETKLLAGRGN"
     gene            218..829
                     /gene="pdxT"
                     /locus_tag="VFU90_11530"
     CDS             218..829
                     /gene="pdxT"
                     /locus_tag="VFU90_11530"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5480460.1"
                     /translation="MQPVVGVLALQGDVVEHVAALERAGARARAVKTREQLGGVDALI
                     VPGGESTTVMKLLRRFELCEPIVERVRAGMPFWGTCMGLIVAAHDVADLDQPTLDLID
                     VTVRRNAFGRQNESAEVELAIPVLGATPFPAVFIRAPWIERAGPRVEILARREGHGVM
                     VREGNVLGTAFHPELAGDSRVHEYFLEMIRRPNAHVGKGSTAA"
     gene            840..1133
                     /locus_tag="VFU90_11535"
     CDS             840..1133
                     /locus_tag="VFU90_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480461.1"
                     /translation="MTSRAEAPGEAQGGFQGEIPPVEPLLAEVIATLSLAAHAYLTES
                     EGKEPDRESAEIAIDVAGAAFERVKDRLRPDERLAITQMLTETRMTFVRKRGT"
     gene            1177..>1556
                     /locus_tag="VFU90_11540"
     CDS             1177..>1556
                     /locus_tag="VFU90_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5480462.1"
                     /translation="MSEVLVLNFTYEALNVTSFQRAVKMIFAGKAEMLHGRDRILSST
                     TCEMRMPSIIRMLYYIRRPMQKVALTKKNVLIRDDHLCQYCGTHGEKLMTVDHVIPKS
                     RGGPSTWENLVCACMRCNNRKNNRT"
BASE COUNT          299 a          504 c          498 g          255 t
ORIGIN      
        1 gccgaaggca tcttcgtggg cagcggaatc ttcaaatccg ccgaccccgc caaattcgcc
       61 aaagcgatcg tcgacgcgac cacgcatttc aacgatccga aagtcgtgac ggacgcgtgc
      121 cgctcgctgg gcgggtccga cgcgatggcc ggtctcgacc tgcgaacgct ggacgaaacg
      181 aagctgctgg ccggccgcgg aaattaaggc ggcggcgatg cagcccgtgg tcggagttct
      241 cgccttgcag ggcgacgtgg tcgaacacgt cgccgcgctc gaacgcgccg gcgcgcgcgc
      301 gcgcgcggtc aagacgcgcg agcagctcgg cggcgtcgac gccttgatcg tgcccggcgg
      361 cgaatcgacc accgtcatga aactcttgcg gcgattcgag ctgtgcgagc ccatcgtcga
      421 gcgcgtccgg gcgggcatgc cgttttgggg gacctgcatg ggtttgatcg tggcggcgca
      481 cgacgtagcg gatctcgatc agccgacgct cgatctcatc gacgtcaccg tgcgccgcaa
      541 cgccttcggg cgtcagaacg aatcggccga ggtagaactt gcgatccccg tactcggtgc
      601 gacgccgttt cccgcggtct tcatccgcgc tccctggatc gagcgcgcgg gaccgcgcgt
      661 cgagatcctc gcccgccgcg agggccacgg cgtgatggtg cgggagggga acgttctcgg
      721 gaccgcgttt catcccgaac tcgccggcga ttcccgggta cacgagtact ttctcgaaat
      781 gatccggcgc ccgaacgcgc acgtagggaa gggctctacc gccgcgtaac cgtccatcca
      841 tgacttcgcg ggccgaggct cccggagaag cgcagggagg atttcaaggc gagatcccgc
      901 cggtcgagcc gttgctcgcc gaggtgatcg cgaccctctc ccttgccgct cacgcgtatc
      961 ttaccgaaag cgaaggcaag gaacccgatc gcgaatccgc ggagatcgcg atcgacgtgg
     1021 cgggcgcggc cttcgaacgg gtcaaagacc ggctccgccc ggacgaacgg ttagcgatca
     1081 cgcagatgct aaccgaaacc cggatgacgt tcgttcgaaa gcgaggcacc taagacggat
     1141 gcctcgctcg cataatccat cccgggacga aacggcatga gcgaagtcct cgtcttgaat
     1201 ttcacctacg aagccctgaa cgtcaccagc tttcagcggg cggtgaaaat gatcttcgcg
     1261 ggcaaggccg agatgctgca cgggcgcgac cgcatcctct cgtcgaccac ttgcgagatg
     1321 cgcatgccgt cgatcatccg gatgctctat tacattcgcc gtccgatgca gaaggtcgcg
     1381 ctcacgaaga agaacgtgct gattcgcgac gatcaccttt gccagtactg cgggacccac
     1441 ggcgagaagc tgatgacggt cgaccacgtg attcccaaga gccgcggcgg accttcgacc
     1501 tgggaaaacc tggtctgtgc gtgcatgcgc tgcaacaacc gcaagaacaa ccggac
//
LOCUS       DASUCJ010000972         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_942740, whole genome shotgun sequence.
ACCESSION   DASUCJ010000972 DASUCJ010000000
VERSION     DASUCJ010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFU90_11545"
     CDS             complement(<1..348)
                     /locus_tag="VFU90_11545"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5480463.1"
                     /translation="MTSKVFLAIRVPAEPARAFEAFTTEIAAWWRPDGLFRITPGGDG
                     RLAFEAGVGGRLYTTLDDGEEFEIGRISVWEPGSRLVFAWRHANFAPDQSTEVEVCFE
                     AVGDETRVSIEHRA"
     gene            complement(345..677)
                     /locus_tag="VFU90_11550"
     CDS             complement(345..677)
                     /locus_tag="VFU90_11550"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5480464.1"
                     /translation="MNTARLDRTLAALAVPCRRQIVELLRERPRRAGELAEATRTSFP
                     AVSRHLRALRESGLVDEKRDAFDSRVRVYSLHARPMAELKAWLEQTDAMWARQLDAFR
                     AHVGKTRR"
     gene            complement(674..>1155)
                     /locus_tag="VFU90_11555"
     CDS             complement(674..>1155)
                     /locus_tag="VFU90_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480465.1"
                     /translation="LQEPPVSNPEFRRPSFIPSVTYDDSRAALQWLQEAFGFEPSEVL
                     TDADGNIVHAEMNHGDGVVMIGNEWADWTRSPQSVDGKNTQRVHVRIDRDIDAHCQRA
                     RRAGARIEMEPADQFYGDRTYMAVDPEGHHWTFAQPVKELSLKDMEDASGFKFQKLK"
BASE COUNT          176 a          404 c          374 g          201 t
ORIGIN      
        1 ggcacggtgc tcgatcgata cgcgggtttc gtcaccgacg gcctcgaagc acacctccac
       61 ctcggtcgac tggtccggtg caaagttcgc atgccgccag gcaaagacaa gccggctacc
      121 gggttcccac accgaaatgc gcccgatttc gaactcctcg ccgtcgtcga gcgtcgtgta
      181 aagccggccg ccgacacccg cttcgaacgc gagccgccca tcgccgcccg gcgtgatccg
      241 gaacaggccg tccggccgcc accacgcggc gatctcggtg gtgaacgctt cgaacgcgcg
      301 cgcgggctcc gcgggcaccc ggatcgcgag gaagaccttc gacgtcatcg ccgggtcttc
      361 cccacgtgcg ccctgaacgc gtcgagttgg cgcgcccaca tcgcgtcggt ctgctcgagc
      421 cacgccttca attcggccat gggccgcgca tgcaagctgt acacgcgcac ccgcgaatcg
      481 aatgcgtcac gcttctcgtc gaccagcccg ctctcgcgca gcgcgcgcag gtgcctgctc
      541 accgccggga acgacgtgcg ggttgcctcg gccagttcgc cggcgcggcg tggacgctcg
      601 cgcaacaatt cgacgatctg ccgccggcac ggcacggcga gcgcggcgag cgtgcgatcg
      661 agcctcgcgg tgttcacttc agtttctgga acttgaaccc ggacgcgtct tccatgtcct
      721 tcagggacag ttccttgacc ggctgcgcga aggtccagtg atggccctcc ggatccacgg
      781 ccatataggt gcgatcgccg tagaactgat cggcgggctc catctcgatc cgtgcgccgg
      841 cgcggcgcgc tcgctggcag tgcgcgtcga tgtcgcgatc gatgcgcacg tggacgcgct
      901 gggtgttctt gccatccacg gactgcgggc tgcgcgtcca gtcggcccac tcgttgccga
      961 tcatcacgac cccgtcgccg tggttcattt cggcgtggac gatgttgccg tcggcatcgg
     1021 tcagcacctc gctgggttcg aaaccgaagg cctcctgcag ccattgcagc gctgcgcggc
     1081 tgtcgtcgta ggtgaccgac gggatgaaag acgggcggcg gaattcggga ttcgacacgg
     1141 gcggctcctg tagtt
//
LOCUS       DASUCJ010000973         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_71737, whole genome shotgun sequence.
ACCESSION   DASUCJ010000973 DASUCJ010000000
VERSION     DASUCJ010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71737"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFU90_11560"
     CDS             <1..1024
                     /locus_tag="VFU90_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5480466.1"
                     /translation="VAQGQLDEGINEQSRIVEQATDKVAQLTETINAIAAAAEEQTRS
                     LDETSLAVTNMATSIEQVTAQVDSLSSISTETSETAQRGGSAIGTIVDGMNTIRSTID
                     ELGGDIRQLGSNSEQIGDIVKVIDRIAEQTNLLALNAAIEAARAGEHGRGFAVVATEI
                     RKLADGSVQATKEIAAHILSTQGVINEVVSAMERLTERVEEGVSSTNSASGALHEIVE
                     AVLGANQQIAEISSVARAMSSNSYQVIRSIEEITRSVQLNVQATQAMSTHSDEVNAAF
                     RNIAEISQQNASSVEVLTYVNKEVTDAAQRMLESVTQMNDFAGRIDGRLAQFKVNESG
                     GEEESV"
     gene            1021..>2128
                     /locus_tag="VFU90_11565"
     CDS             1021..>2128
                     /locus_tag="VFU90_11565"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU5480467.1"
                     /translation="MSLRVRLLGTIVGAILLFFIISVVAARLTLHKDLMDLGSTEVTN
                     GSNAFAGYWDSRKDQIRLLIAQDAVSDALRKNLQTHDSKALQDQLSNIARTSGLSFLT
                     IVDDNGNVVARANGGTGGSLAQNTYVQRALSGETISSAAIMPPNDLQGEGLQPQAEAD
                     VQSDGKVVEHVKKGLAIIAAAPMSDTNERTLGAIYGGVLMNHFYDLVDQSTHALGGQT
                     AVLDGDAIVSSTISESDGTRVIDSQVQQYDAQKLAAGTAWVGTDAEGGTRYLARLDPI
                     TDDQNDVIGARWYGVPLAQITGIINHMTEVFILWGLLAMVIALAVAVPFVQRISNAIA
                     KRSEQVSVAAKDLGVTIVGGEVSGDHVAATKAAVE"
BASE COUNT          456 a          657 c          672 g          343 t
ORIGIN      
        1 ggtcgcacag ggacagctcg acgaaggcat caacgagcag tcgcgcatcg tcgagcaagc
       61 caccgataag gtggcgcagc tcaccgaaac catcaacgcc atcgccgcgg ccgcagaaga
      121 gcagacgcgc agcctcgacg agacctcgct cgcggtgacg aacatggcga cgtccatcga
      181 gcaggtcacc gcgcaagtag attcgctctc gtcgatctcc accgagactt cggaaacggc
      241 gcagcgcgga ggaagcgcga tcggcacgat cgtcgacggc atgaacacca ttcgatcgac
      301 gatcgacgag ctgggcggcg acatccgcca actgggaagc aactccgaac agatcggcga
      361 catcgtcaaa gtgatcgatc gcatcgccga gcagacgaat ctgctcgcgc tcaacgcggc
      421 gatcgaggcg gcgcgcgccg gcgagcacgg ccgcggcttc gccgtcgtcg caacggagat
      481 tcgaaagctc gccgacggca gcgttcaggc gaccaaagag atcgccgccc atattctctc
      541 gactcagggc gtgatcaacg aagtggtcag cgcgatggag cggctcaccg agcgcgtgga
      601 ggaaggcgtc tccagcacca actccgcatc gggcgcgctg cacgaaatcg tcgaagcggt
      661 gctcggcgca aatcagcaaa tcgccgagat atcctcggtg gcacgcgcca tgagcagcaa
      721 ctcgtatcag gtcattcgat cgatcgagga gatcacgcgc tcggttcaat tgaacgtcca
      781 ggcgacgcaa gcgatgtcca cgcactccga cgaggtgaat gccgcgttcc gcaacatcgc
      841 ggagatctcg cagcagaacg cttcgtccgt cgaggtgctg acctacgtga acaaggaagt
      901 caccgacgcc gcgcagcgca tgctcgaatc ggtgacgcag atgaacgatt tcgccggccg
      961 catcgacggg cgcctggcgc aattcaaagt aaacgaatcc ggtggagagg aagagtcggt
     1021 atgagcctgc gcgttcgatt gctcggcacc attgtcggcg caatcttgtt gttcttcatt
     1081 ataagcgtcg tcgccgcgcg cttgacgctg cacaaagatc tcatggatct cgggagcacc
     1141 gaggtgacga acgggtccaa cgcctttgcg gggtattggg actcgcgcaa ggatcagatt
     1201 cgcctgctga tcgcgcagga cgccgtctcc gacgcgctgc gcaagaatct gcagacgcac
     1261 gattccaaag cgctgcagga tcagctctcc aacatcgcgc gcacgtcggg tctttcgttt
     1321 ctgacgatcg tcgacgacaa cggcaatgtg gttgcgcgcg cgaacggcgg taccggcgga
     1381 tcgctcgcgc agaacacgta cgtacagcgt gcgctgagcg gcgagaccat cagcagcgcc
     1441 gccatcatgc cgccgaacga tctgcagggt gaaggattgc agccgcaggc cgaagccgac
     1501 gtgcagagcg acggcaaggt ggtcgagcac gtcaagaaag gcctggcgat tatcgcggcc
     1561 gcgccgatga gcgataccaa cgagcgcacg ctgggcgcca tctacggcgg cgtgctgatg
     1621 aaccatttct acgatctggt cgatcagagc acgcacgcgc tcggcggaca aaccgcggtt
     1681 ctcgacggcg atgcgatcgt gtccagcacg atcagcgagt ccgacggtac gcgcgtgatc
     1741 gattcgcaag ttcagcagta cgatgcgcaa aaactcgctg ccggtacggc gtgggtcggt
     1801 acggacgctg aaggcggcac gcggtatctg gcgcgcctgg atccgattac cgacgatcag
     1861 aacgacgtca tcggcgcacg ctggtacggc gttccgctgg cgcaaatcac cggcatcatc
     1921 aatcacatga ccgaagtgtt catcctttgg ggcttgctcg cgatggtcat tgcgctggcc
     1981 gttgccgtcc cgttcgttca gcgcatctcc aacgctatcg ccaagcgcag cgaacaggtc
     2041 agtgtcgccg ccaaggatct gggcgttacg atcgtcggcg gcgaggtctc aggcgatcac
     2101 gttgccgcga ccaaggccgc ggtcgagc
//
LOCUS       DASUCJ010000974         7494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_850718, whole genome shotgun sequence.
ACCESSION   DASUCJ010000974 DASUCJ010000000
VERSION     DASUCJ010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850718"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..2975)
                     /locus_tag="VFU90_11570"
     CDS             complement(264..2975)
                     /locus_tag="VFU90_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEU5480468.1"
                     /translation="MLAALTLCITPVAQVRADQPASPGNSAEHRKSPADHGGGAHAFF
                     TPEVMKQRGFIPPGLAKKPGVQQTQGDNSGQGPMLYHGGSVMRDVTNYVIIWNPPGST
                     FSATYQSLIEQYFKGVGSTPFAEIYAQYGDNTGAPVPSSSHFGGTWVDTTNAYPHTGT
                     VADPLVGSDIQAEVARAITANPTWQGPGLSTMYFVYLGQNIIECFKGSGNTFGCFDGV
                     DSGGNVPPRPSGNPNTVAGAGTYCAYHSTSGSSIYATMPYAGSSTHCGSQPAYPNGHD
                     QDLVLSPTSHEQFEAYTDPHLDAWYDSTGTGGEIGDKCAYTYGQIEPDGTTDVISGLR
                     YQLQEEWSNDLTYGCIKRAGTAPQLAIAGNLDFGLVPRGTVATRTVLVQNTAGGDLDL
                     LNLRIGTAGAGFSIDPSTPLWGTLPAGDSVSVLVNFAPSATATSPTPSPDSLVVDTDQ
                     IGYETQSLATSATVGLPQAAVSGGLNFGTVCNGSTVDQTITVTNTGQAPLTINSVTIG
                     GAATPGLSVLPSPSLPQTIAVGASLTFTVRFAPSGVGGGPIAGTVVVATDDPNNPSIS
                     VPISGTVGAANVTISSSALDFAGVATDNRTSPSTADRTVTIGNTGSCALGLSSLSITG
                     PNAGDFSIVGAPGLPVSIANASSLTLTVRFNPSAAGARSATLLVGTTDPLNPSKSVAL
                     TGTGLVPAIQTSAATLNYGPTVILSQAPGYTGTTTALRITNVGQSELIVDTMTSTAPF
                     GAPGAASPPARFAPSDGFNEPVTFAPTTVGKFVGSFTVSDNDPEGGATRTVPLCGEAV
                     MRGIRVLAVAANGAPFASIAKLHLQSHGTAQTININAQNLALAGVATSCDPAAQMQYQ
                     NQGLPAAGTVNQRSSYYTLSVTAGGKSTTITFTLGVAEFKTLVVTVK"
     gene            3272..6439
                     /locus_tag="VFU90_11575"
     CDS             3272..6439
                     /locus_tag="VFU90_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5480469.1"
                     /translation="MVRRGRLYPLLEAARGQALWIAAPAGYGKTTLAARFVADEARRS
                     VWYRTDAGDADPAAMFHYLERVVRKGNAAALPRFGSEYGDRLEMFAQRFFRAFYACLP
                     DRAIVVLDDLHAANERRLAQVIGPAIAELPPAIQLVLLSRALPPAELVSAEAHEQLRV
                     IDGSMLAFTPEETREVAELRLPAPLAHAEAGRLYRLTRGWPAALVLVCAALARDPTAV
                     DSLVEYSREAAFGLLTREVFAALPRHERRFMLLTSLLGQVTPTTAAAMTGRRDAQEML
                     DSFVARGMFVIRVAGEGPAYRYHELLRDFLRSPGVALSAGAMRESVEAGICSLAAEGE
                     WDDAIALALAQGRSDLAAPLLAQHAPTLVAQGRRATLQDAARRLSVPWPAPYPELAYW
                     IGVTHMIGDEQEACRWLERAWVEFTRAGDDGRARLAAAQAVLAIHMSWHTYVVKDTWL
                     ERLARSDASVCAALSRGEMIRIATARLRAAEMDDSGALDPVDTARRADELLELLEAPG
                     AGIHVDDRFIAADALQEYASISGRRDVFERAAAAVLPERDHPDLSPWAKLHWLISFGT
                     IIGRRFPCRPRGTPYATGEEALAEAQKLASREGLQNLEFSATFARCAVAIARGDGHEL
                     RAQVAYLDRIADARYPMQVSNLMQWKATVALADRNHPAALEACAAAVEAGVRGDLPRG
                     QMWSIRLTEAQALMAAGRNTAAASLMDTEAVHHTGHFRRLCELVAQTARLRERRGSAE
                     DYRRDLWRLVCAIRENGWVNYLSAAPEIATEIWANALAESIEVQFVTDAIRARRLKPP
                     RVPIGAWPWTLRVHLLGGFAVEIRGEPLRFDGKVQRKPLELLRFIAAQHPAPAPIDDA
                     IAALWPNADRRAARASLDVAVHRLRKLLGDGDLLVAADGSLSLDPDRSWVDAGAIAQW
                     ADEIRCRLESAADSATLDLATRLVTGYRGALLGSERPPAWAVARREMIHSHFVELAAS
                     LGSFHERAGDFDAARAVYDHAVSVEPLAEVLYRGLMRCHGAAGETAEVLRSFRRCREV
                     LSIVLGIGPASETVNLLARLCEIAG"
     gene            6527..>7494
                     /locus_tag="VFU90_11580"
     CDS             6527..>7494
                     /locus_tag="VFU90_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5480470.1"
                     /translation="MLVAMPIEPGLDRTQLRELAQLAYSDAAGARVARRARTILLAAE
                     GVSDVRIARELGVGRAQVARWRNRFVEGGWSAVACDRPRSDRPATIDVERILASIRGE
                     DGLGPAPTVGGIAKLCGVSEASVARVRRRYGLARPRPRAPSATEACATGVGPAPLGVF
                     LAARCRVLAFDAQGVTSGAKGERPAGSDAAHLRARRPTRVDDGDGEVLAYLSFLDRVA
                     AACSRERGAALVCESDAVLSLSSLDVWRGRNPYVAFEIADSSLDWRRRIDRLLALRRR
                     ASPMDGREAALHAADDAAGDLDVPEIVSALLDYFTVARCHPTPFVWV"
BASE COUNT         1117 a         2469 c         2627 g         1281 t
ORIGIN      
        1 gaacggtgtg ctcgcgatgc tcgcggcggc ctggtactac cgccgccttc cgggggtgcg
       61 cagggtgctg cgcccgatgt acgaagagct tggcgtaccc ggcgggcgct gacgctcgca
      121 gccgctcgag ccccggcgcg cgcattgtcg ccggtcacgg cgaggggcaa tgaaacggcg
      181 cggaccgttt ccggtacgcg ccgtcgagtc tcctgagcgg ctgcggcagg ctccccagtg
      241 gggtgcttgc cgcgccgtta ccgctacttc accgtgacga cgagcgtctt gaactcggcg
      301 acgccgagcg tgaaggtgat cgtcgtcgac ttgccgccgg ccgtgacgga cagagtgtaa
      361 tacgagctgc gctggtttac cgtgcctgcg gccggaagtc cctggttctg atactgcatc
      421 tgtgccgcgg gatcgcacga ggtcgcgacg ccggcgagcg ccagattctg cgcattgatg
      481 ttgatggtct gcgcggtgcc gtgcgattgc aggtgcaact tggcgatgct cgcaaacggc
      541 gcgccgttcg cggcgactgc aaggacgcga atgccgcgca ttaccgcctc gccgcacagt
      601 ggcaccgtcc gcgttgcacc gccttccgga tcgttgtccg acacggtgaa cgagccaaca
      661 aacttcccga ccgtcgtcgg cgcgaacgtc accggctcgt tgaagccgtc gctcggtgcg
      721 aagcgagccg ggggactcgc cgctcctggg gcgccgaacg gagcggtgct cgtcatcgta
      781 tcgacgatca gctccgactg tccgacgttc gtaatcctga gcgcggtcgt cgtcccggtg
      841 tagccgggtg cctgcgacag gatcaccgtc gggccgtagt tgagcgtcgc ggccgacgtc
      901 tggatcgccg gaacgaggcc tgtgccggtc aatgcaaccg atttcgaggg attgagcgga
      961 tcggtcgtcc caacgaggag cgttgccgag cgtgcacccg ccgcactcgg attgaaccgc
     1021 accgtgagcg tgaggctgct cgcgttcgcg atcgaaaccg gcagtcccgg cgctccgacg
     1081 atcgagaaat cacctgcgtt cgggccggtg atcgacagcg atgagaggcc gagcgcgcag
     1141 cttccggtgt tgccgatggt caccgtgcga tccgccgtgc tgggcgaggt tcgattgtcg
     1201 gtcgcgacac cggcgaagtc gagcgcgctc gagctgatcg tgacgtttgc tgctccgact
     1261 gtgccgctga tcggaaccga gatcgacgga ttgttcgggt cgtccgtcgc gacgactacg
     1321 gtgccggcaa tcgggccgcc accaacaccc gaaggagcga agcggacggt gaaagtgagg
     1381 ctcgcgccga cggcgatcgt ctgcggaagg ctcgggctcg gcagcaccga aagtcccggt
     1441 gttgcggccc caccgatcgt cacgctgttg atcgtcagcg gtgcctgacc ggtgttggtg
     1501 acggtgatcg tctgatctac cgtggaaccg ttgcacacgg tcccgaagtt gagcccgccg
     1561 ctgaccgctg cctgcggcag gccaacggtc gccgatgtcg caagcgactg agtctcgtag
     1621 ccgatctggt cggtgtcgac gaccagcgaa tcgggactcg gtgtgggcga tgtcgcggtc
     1681 gcgctcggcg cgaaattcac cagcaccgac acgctgtcgc cagccggcag ggtaccccat
     1741 aacggtgtcg acggatcgat gctgaatccg gcgccggcgg taccgatgcg caggttcaga
     1801 aggtcaaggt cgccgccggc ggtgttctgg acgaggacag tccgcgtcgc aacggtgccg
     1861 cgcggaacca gtccgaagtc gagattgccg gcaatcgcga gctgcggcgc cgttccggcg
     1921 cgcttgatgc agccataggt gagatcgttg ctccattcct cctgcagctg gtagcgcagg
     1981 ccggagatga cgtcggtcgt tccgtcgggc tcgatctggc cgtaggtata cgcgcatttg
     2041 tcgccgatct cgccgccggt gccggtcgag tcgtaccacg cgtcgaggtg cgggtcggta
     2101 tacgcctcga actgctcgtg cgacgtgggc gacagcacga ggtcctggtc atgtccattc
     2161 gggtacgcgg gctggctgcc gcagtgggtg ctgctcccgg catagggcat cgtcgcgtag
     2221 atcgagctcc ccgaagtgct gtggtaggca cagtaggtac cggcacccgc aacggtgttc
     2281 ggattgcccg atggacgcgg cggaacattg ccgccgctgt cgacgccgtc gaagcagccg
     2341 aacgtgttgc ccgagccctt gaagcactcg atgatgttct ggccgagata cacgaagtac
     2401 atcgtggaga gccccgggcc ctgccacgtc ggattcgcgg tgatcgctcg cgctacttcc
     2461 gcctggatat ccgacccgac cagggggtcc gctactgtac ccgtgtgggg atacgcgttc
     2521 gtggtgtcga cccacgtgcc accgaaatga ctgctgcttg gaacgggtgc gccggtgttg
     2581 tcgccgtatt gcgcgtagat ctccgcgaac ggtgtgctgc cgactccctt gaaatactgc
     2641 tcgatcagtg actggtacgt cgcgctgaac gtcgagcccg gcggattcca gatgatgacg
     2701 tagttggtga cgtcgcgcat gaccgagccg ccgtggtaga gcatcggccc ctggcccgag
     2761 ttgtcgccct gtgtctgctg gaccccgggc ttcttcgcga ggccgggtgg aatgaagccg
     2821 cgctgcttca tgacctcggg agtgaagaac gcgtgtgcgc ctccgccgtg gtcggccgga
     2881 gacttgcgat gctcggccga gtttcccggc gaggcgggct gatcggctcg cacctgcgca
     2941 acgggcgtga tgcacagcgt cagtgcggcg agcaaggctg tatgccacag ggttccaatc
     3001 gccgccctgc gcggggtctc ccgcctacgt tggcaatcct gaaggtgact tggcgcgtgc
     3061 atgacgatct ccccatttgc gttttcgagc acagcgccaa aaagattatt cccgttgacg
     3121 ccgacggcaa cgtcgcggca atctgtcaaa aataacgaag catcgcaccg gcgatacttc
     3181 gacgaagaac gccgacccga cgatgccagg caagcccatg tcacgcagcc ccaggccacc
     3241 ggtcaagctt tcgccgccaa ggctcggccg cgtggtgcgc cgcgggcgcc tgtatcccct
     3301 gctcgaggcc gctcggggcc aggctctgtg gatcgcggca ccggccggat acggcaagac
     3361 gaccctcgcg gcccggttcg tcgccgacga ggcgcgtcgc agcgtctggt atcgcaccga
     3421 tgcgggcgac gccgatcccg cggcgatgtt ccactacctc gaacgcgtgg tgcggaaggg
     3481 gaacgctgcg gcgctgccgc gcttcggatc ggaatacggt gaccggctgg agatgttcgc
     3541 gcagcgcttc tttcgcgcgt tctacgcgtg cctgccggac cgggcgatcg tagtcctcga
     3601 cgacctccac gcggcgaatg agcggcggct cgcgcaagtc atcggtccgg cgattgccga
     3661 gctcccgccg gcgattcagc tcgtcctgct ctcgcgggca ctgccgcccg cagagctcgt
     3721 ctcggccgag gcgcacgagc agcttcgggt gatcgacgga tcgatgctcg cgtttacgcc
     3781 ggaggagacc cgcgaggtcg ccgagctccg actccctgcg ccgctcgcgc atgcggaggc
     3841 ggggcgtcta taccggctca cccgcggctg gccggccgca ctcgtcctcg tgtgcgccgc
     3901 gctcgcccgc gacccgacgg cggtcgattc gctcgtcgaa tattcgcgcg aagctgcgtt
     3961 cggcctgctg acgcgtgaag tgttcgcggc cttgcccagg catgaacgtc gcttcatgct
     4021 gctgacctcg ctgcttggtc aggtgacacc tactactgcc gccgcaatga ccgggcggcg
     4081 cgacgcgcag gagatgctcg actcgttcgt tgcgcgagga atgttcgtga tccgcgtcgc
     4141 aggcgaaggt cccgcgtacc gctatcacga attgctgcgc gattttctgc gcagccccgg
     4201 ggtcgcgctc tcggcgggcg cgatgcgcga gtccgtcgag gcgggcattt gctcgctggc
     4261 agccgagggc gaatgggacg acgcgatcgc cctcgcgctc gcccagggac gaagcgacct
     4321 cgcggcgccc ctgctggcgc agcatgcgcc gacgctcgtt gcgcagggtc ggcgggccac
     4381 gcttcaggac gccgcacgca ggctttccgt gccgtggccg gctccgtacc ccgagctcgc
     4441 ctactggatc ggcgtgaccc acatgatcgg cgatgagcag gaagcctgcc ggtggctcga
     4501 acgggcgtgg gtcgagttca cccgcgcagg cgatgacggg cgggcgcggc tcgccgccgc
     4561 gcaggcggtg ctggccatcc atatgagctg gcatacgtac gtcgtcaaag acacgtggct
     4621 cgaacgcctg gcgcggtccg atgcgtcggt ttgcgctgcg ctctcgcgcg gcgagatgat
     4681 ccgcatcgcc acggcgcgcc tgcgcgctgc ggaaatggac gattcggggg ccctcgatcc
     4741 ggttgatacg gcgcggcggg ccgacgagct gctcgaactc ctcgaagcgc ccggcgcagg
     4801 gatccacgtc gacgatcgat tcatcgccgc cgacgccctg caggagtacg catcgatctc
     4861 cggcagacgc gacgtcttcg agcgggccgc cgccgcggtg cttccggaac gggatcaccc
     4921 cgatctctct ccctgggcaa agctccactg gctgatctcg ttcggcacga tcatcggtcg
     4981 gcgctttccg tgtcgcccgc gcggcacgcc ctacgcgacc ggcgaggaag cgctcgccga
     5041 agcacagaag ctcgcgagcc gcgaaggcct gcagaacctg gagttctcgg ccaccttcgc
     5101 gcgctgcgcg gtcgcgattg cccgcggtga cgggcacgag ttgcgcgcgc aggtcgccta
     5161 tctcgatagg atcgccgacg cacgctatcc gatgcaggtg tcgaacctga tgcagtggaa
     5221 ggcaacggtt gcgcttgcgg accgcaacca tccggcggcg ctggaagctt gcgcggcggc
     5281 cgtggaagcc ggggtgcgcg gcgaccttcc gcgcggtcag atgtggtcga tccggctcac
     5341 cgaagcgcag gcgttgatgg cggcgggtcg caacacggcc gcagcgtcgc tgatggatac
     5401 cgaagccgtt catcacaccg gacactttcg caggctgtgc gagctcgtcg cgcagacggc
     5461 gaggctgcgc gagcggcgtg gaagcgccga ggactatcga cgtgaccttt ggaggctcgt
     5521 ttgcgcgatc cgcgaaaacg gctgggtgaa ttacctcagc gcggcccccg agatcgccac
     5581 ggaaatctgg gccaacgcgc tggcggaatc gatcgaagtg cagttcgtga ccgacgccat
     5641 ccgggcgcgc aggttgaagc cgccccgcgt gccgatcggc gcgtggccgt ggacgctgcg
     5701 tgtgcacctg ctgggcggct tcgccgtcga gatccgcggc gagcctcttc ggttcgacgg
     5761 gaaggtgcag cgtaaaccgc tcgagctgct tcgcttcatc gccgcgcagc accccgcgcc
     5821 ggcaccgatc gacgacgcga tcgcggcgtt gtggccgaat gcggaccggc gggcggcgag
     5881 ggcgtcgctc gacgtcgccg tgcatcggct gcgcaaactg ctcggcgatg gcgacctgct
     5941 ggtcgcggcc gacggcagtc tttcgctcga cccggaccgc tcgtgggtcg acgcaggggc
     6001 catcgcgcaa tgggccgacg agatccggtg ccgcctcgaa agcgcagcgg acagcgcaac
     6061 actggatctg gcgacccgtc tcgtcaccgg ctatcgcggc gcgctgcttg gctcggagcg
     6121 gccgcctgcg tgggctgtcg cgcggcgaga gatgatccac tcacacttcg tcgagcttgc
     6181 ggcgagcctc ggcagtttcc acgagcgcgc aggcgatttc gacgcggcgc gcgcggtcta
     6241 cgaccacgcg gtctcggtcg agccgctcgc cgaggtgctc tatcgcggcc tcatgcgctg
     6301 ccacggcgca gccggggaaa cagccgaagt gcttcgctcg ttccgccgct gccgcgaagt
     6361 cctttcgatc gttctcggca tcggtccggc ctccgagacg gtgaatctgc tggctcggct
     6421 gtgtgagatc gccgggtgac ccgcggcact gcccgcatgc gggaaaattc ggaatccgaa
     6481 aggcatcggt aagcgacgga acgtatccac gcgatcgacg cgttctatgc tcgtcgcgat
     6541 gccgatcgaa cccggactcg accggacgca acttcgcgag ctcgcgcaac tggcctactc
     6601 ggacgccgcc ggcgccaggg tcgccaggcg cgcgcgcacg atcctgctcg cggccgaggg
     6661 tgtgtccgat gtgcggatcg cacgcgaact cggcgtgggt cgcgcgcagg tggcccgctg
     6721 gcggaatcgc tttgtcgaag ggggatggtc ggcggtggcg tgcgatcgac cgcgcagcga
     6781 ccggcctgca acgatcgatg tcgaacgcat tctcgcgtcg atccgcggcg aggatgggct
     6841 gggcccggcg ccgacggtcg gcgggattgc gaagctttgc ggggtcagcg aggcgagcgt
     6901 cgcgcgcgta cggcgccgtt acgggctggc ccggccccgc ccacgagcgc catccgccac
     6961 cgaggcttgc gcgacgggcg taggtccggc gccgctcggg gtgttcctgg cggcgcgctg
     7021 ccgcgtgctc gcgttcgacg cgcagggcgt cacctcgggc gcgaagggcg agcgcccggc
     7081 aggctccgat gccgcgcacc tccgtgcgcg acggccgaca cgcgtcgacg acggagacgg
     7141 tgaagtgctc gcgtatctga gctttctcga tcgcgtcgca gcagcgtgct cgcgcgaacg
     7201 cggagcggcg ctcgtctgcg agagtgatgc ggtgctgtcg ttgtcctcgc tcgacgtctg
     7261 gcgcgggcgc aacccgtacg tcgcgttcga gattgcggat tcgtcgctgg actggaggcg
     7321 tcgcatcgac cgcctcctcg cgctgcgccg cagagcgtca ccgatggacg gccgggaagc
     7381 tgccctccat gccgcggatg acgctgccgg tgacctcgat gttccggaaa tcgtctccgc
     7441 gctgctcgac tacttcaccg tcgcgcggtg ccatccgacg ccgttcgtct gggt
//
LOCUS       DASUCJ010000975         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_789684, whole genome shotgun sequence.
ACCESSION   DASUCJ010000975 DASUCJ010000000
VERSION     DASUCJ010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789684"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU90_11585"
     CDS             complement(<1..306)
                     /locus_tag="VFU90_11585"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5480471.1"
                     /translation="MHPEVRSLEPGSCPICGMALEPVVASADAAPNPELADMSRRFWI
                     GLALALPVFALEMGAHVPALRTSEVISPALSGWISLALATPVVLWAGWPFFQRAWASL
                     "
     gene            759..1196
                     /locus_tag="VFU90_11590"
     CDS             759..1196
                     /locus_tag="VFU90_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480472.1"
                     /translation="MTTTGTDRASALQLAPYIFFYGRCEEALGFYKGVFGGTYELQRN
                     ADSPMADEVAPDFRNKVMHAHFTAPGVTFMCSDGRESKTIDPEEGNVSLSLTASDADE
                     GKRVFDALAAGGTVVAPYEDAFWGGKFGFVNDRFGTQWLISGP"
     gene            1349..1741
                     /locus_tag="VFU90_11595"
     CDS             1349..1741
                     /locus_tag="VFU90_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5480473.1"
                     /translation="MVIVRANEESEAGVLPSADLLAAMNEFNQGLVRDGVLLAAEGLQ
                     ASSKGARMKFGGPKPVVTDGPFAETKELIAGFWLVQGKSKEEIIERFRATPFTNGEEI
                     EIRQAFEAEDFGDAVSPEAREFEARQRG"
     gene            1738..>2330
                     /locus_tag="VFU90_11600"
     CDS             1738..>2330
                     /locus_tag="VFU90_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5480474.1"
                     /translation="MNQSDFAAAMDAVWRIESPKLIAFLSRTCRDVGVAEEIAQDAFV
                     AALTQWPETGVPRAPGAWLTAVAKRRAIDRLRRERTLERKHAELGRELEPEPSADAAL
                     DEPHGDDLLGLMFVACHPVLSREARVALTLRLLGGLTTAEIARAFLVSEPTIAQRIVR
                     AKRTLAEARVPFELPGGAERARRLDSVLEALYLIFNEG"
BASE COUNT          444 a          771 c          739 g          376 t
ORIGIN      
        1 cagcgacgcc caggcacgct gaaaaaacgg ccagccggcc cacagtacga ccggcgtcgc
       61 caacgcgagt gaaatccagc cggaaagggc cggcgaaatt acttcgctcg ttcgcagcgc
      121 cggaacgtgc gcgcccattt cgagcgcgaa gacgggcagc gcgagcgcga ggccgatcca
      181 gaaacgccgg ctcatgtcgg ccagctccgg attcggtgcg gcatcggctg aggctacgac
      241 cggctccagg gccatgccgc agatcggaca gctccccggc tctaggctgc gaacctccgg
      301 gtgcatcgga caggtatagc cgtcacgcaa ttcgcccgcg cacgttccat ggcttcctcg
      361 gctcctcctg gaatcgtctt accgaacgat caccgccacg atttgactcg ccgcacgctc
      421 cacgctgcga tgaaccacgg gttgccgcgg cggtcgggcc ctccgccgaa cgtgccgaaa
      481 gactgcgtcc gatcgccgca acgtgcgccg gcacaaatcc aatcggcgcc gggacgcatc
      541 gtagccgtca cgatactcgg cgacaccggc ccgcgaccgg gaggctcgac caggattctt
      601 tataagaagt atacttgcta tttacaagtt tacatgctat agtcggacag cggcgggagc
      661 cgcgccgggc agatcttcgc tcgcccgttc gcgttgacgc gaagacctgc tacgatgcaa
      721 cgactcgagc ataccgaaaa acgaggggag aacatcctat gaccacgacc ggaaccgacc
      781 gagcctccgc actccagctg gccccgtaca tctttttcta cggccgctgc gaggaggcgc
      841 tgggtttcta caagggcgtc ttcggcggaa cctacgagtt acagcgtaac gccgactcgc
      901 cgatggccga tgaggtagcg cccgactttc gcaacaaggt catgcacgcg cacttcaccg
      961 caccgggcgt cacgttcatg tgctccgatg gccgcgaaag caaaacgatc gatcccgaag
     1021 aaggcaacgt atcgctttcg ctcaccgcaa gcgatgccga cgagggcaag cgcgtattcg
     1081 acgcgctggc ggccggtgga accgtcgtcg ccccgtacga ggacgcattc tggggcggaa
     1141 agttcggctt cgtgaacgac cgcttcggca cccaatggct gatttccgga ccctgagcgg
     1201 cacggtagcc cccgccgcgg agttccaagc gcccgaattt tttcgggcgc ttcggatttc
     1261 gtgtcgaatt cggcccgcac cgttcgacgt ctagatagac tacccgttcg aaccattagg
     1321 tgaaaaggag atcgagaaca tgcggttcat ggtgatcgtt cgcgcaaacg aggagagcga
     1381 agccggcgtt ctgccgagcg ccgatctgct ggcggcgatg aacgagttca atcaagggct
     1441 cgtgcgcgac ggcgtgctgt tggcggccga agggctgcaa gccagctcca agggcgcgcg
     1501 gatgaagttc ggcggaccga agccggtcgt caccgacggt ccgtttgccg aaacgaaaga
     1561 gctgatcgcc ggcttctggc tggttcaggg caaatcgaag gaagaaatca tcgagcgatt
     1621 ccgcgccacc ccgtttacca acggcgaaga gatcgaaatt cgtcaagcgt tcgaagccga
     1681 ggatttcggc gacgcagtct cgccggaagc gcgcgagttc gaggcccggc agcgcgggtg
     1741 aatcaatccg atttcgccgc cgcgatggac gcggtctggc ggatcgaatc gccgaagctg
     1801 atcgccttcc tctcgcgcac ctgtcgcgac gtgggcgtcg ccgaagagat cgcgcaggac
     1861 gccttcgtcg cggccctcac gcagtggccc gagaccggcg tcccgcgcgc tcccggcgcc
     1921 tggctgaccg cggtcgccaa acggcgcgcg atcgaccgcc tgcggcgcga gcgcacgctc
     1981 gagcgcaaac acgccgaact cggacgcgag ctcgaacccg agccttccgc cgacgcggcg
     2041 ctggacgaac cgcacggcga cgacctgctc ggcctgatgt tcgtcgcctg ccatccggtg
     2101 ctgtcgcggg aggcccgtgt ggcgctcacg ctgcgcctgc tcggcggttt gacgaccgcc
     2161 gagatcgcgc gcgcgttttt ggtttcggaa ccgacgatcg cgcagcgaat cgtgcgcgcc
     2221 aaacgcacgc ttgccgaagc ccgcgtgccg ttcgaactgc ccggcggcgc cgaacgcgcg
     2281 cggcgcctag actccgtgct ggaagcgctg tatctcatct tcaacgaggg
//
LOCUS       DASUCJ010000976         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_377585, whole genome shotgun sequence.
ACCESSION   DASUCJ010000976 DASUCJ010000000
VERSION     DASUCJ010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377585"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFU90_11605"
     CDS             <1..1051
                     /locus_tag="VFU90_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480475.1"
                     /translation="VANAMRLINAGANSVVAAGGMESMSNAPYLLRDARFGYRFGDGT
                     LVDAMIYDGLWDQYFPMTMASQGSKVAEEMGFTREEQDRFALASHQRATASHEAGHFG
                     EEIVPVKVAAKSKGKVVVEELPRQGRARVPVAAGGAPNTIWGHEPSQQFTFDYAKYAP
                     FVAGDHPATVIDRDEAVRTDASLEAMRKLRPLEKDGTITAGNAPGVNDGAAALILADA
                     EYAARNHLEPLATVVEHATVAWDSPYICLTPAMAAQKLLDRTGLTRSDIAVWEINEAF
                     SAVAIASARNLGLNEEDVNRFGGAVAMGHPIGASGARILGTLINQLRKRGGGLGIATI
                     CSGGGQGDAVLVRVG"
     gene            1063..>1286
                     /locus_tag="VFU90_11610"
     CDS             1063..>1286
                     /locus_tag="VFU90_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5480476.1"
                     /translation="MKIVVVGAGQMGAGIAQVAAASGHEVALNDRSPELIARGIAAIA
                     KNLDRGVEKGRISDQARTETLARIVATPELA"
BASE COUNT          228 a          403 c          461 g          194 t
ORIGIN      
        1 ggttgcaaac gcgatgcgtt tgatcaacgc gggcgcaaat tcagtggtcg ccgccggcgg
       61 catggaatcg atgtcgaacg cgccgtacct cttgcgcgac gcgcgcttcg ggtatcgctt
      121 cggtgacgga acgctcgtgg acgcgatgat ctacgacggg ctatgggatc aatattttcc
      181 gatgacgatg gcgtcgcagg ggagcaaggt cgcggaggag atggggttca cgcgcgagga
      241 gcaagaccgg ttcgcgctcg cgagccatca gcgcgcgacg gccagccacg aagccggtca
      301 cttcggcgaa gagatcgttc cggtcaaggt cgcggcgaaa tccaaaggca aggtcgtcgt
      361 cgaggagctg ccgcgccaag ggcgcgcgcg cgtcccggtt gccgccggcg gtgcgccgaa
      421 tacgatatgg ggacacgaac cgtctcagca gttcacgttc gattacgcga agtacgcgcc
      481 gttcgttgcc ggcgaccatc cggccacggt catcgatcgc gacgaggcgg tacgaaccga
      541 cgcgagcctc gaagcgatgc gcaaactgcg gccgctcgag aaagacggga cgatcaccgc
      601 cggtaacgcg cccggggtca acgacggcgc ggccgcgctg attctcgcgg acgccgagta
      661 cgcggcccgg aaccatctcg aaccgcttgc gacggtagtc gaacacgcga cggttgcgtg
      721 ggattcgccc tacatctgcc tgacaccggc gatggccgcg cagaagctgc tcgatcgcac
      781 cggactgacg cgctccgaca tcgccgtttg ggaaatcaac gaagcgtttt cggcggtcgc
      841 gatcgcctcg gcgcgcaacc tcggtttgaa cgaggaagac gtcaaccgct tcggcggcgc
      901 ggtcgcgatg ggccatccga tcggtgcttc gggggcgcgc atcctcggca cgttgatcaa
      961 ccagctgcgc aagcgcggcg gcggcctcgg gatcgcgacg atttgctccg gcggcggcca
     1021 gggcgacgcc gtgctggtgc gcgtcggtta gcggcgccga ttttgaaaat cgtcgtcgtc
     1081 ggcgccggcc aaatgggcgc ggggatcgcc caagtcgcgg cggccagcgg gcacgaggtc
     1141 gcgctgaacg accggtcgcc cgaactgatc gcgcgcggca tcgcggcgat cgccaagaac
     1201 ctcgatcgcg gcgtcgagaa ggggcggatt tccgatcagg cgcggacgga gacgctggcg
     1261 cggatcgtcg ccacgccgga gctggc
//
LOCUS       DASUCJ010000977         1187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1278999, whole genome shotgun sequence.
ACCESSION   DASUCJ010000977 DASUCJ010000000
VERSION     DASUCJ010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278999"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFU90_11615"
     CDS             <1..612
                     /locus_tag="VFU90_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480477.1"
                     /translation="GTYVLAIIDTVSRVGWIHRSLAFQLHVVSMVFTNGYVATVREPA
                     HAQPRDPSELGPGQRPAALMVASGVAPVAGLAIGAITGGKDGALLGGSIGSAVGFVTL
                     LVAAAHSGEYDLGPGFPLELRSQGPIVLDATRTAEAARMPSRVQHHENTSCYAPGTPG
                     TPDITIPGTPGTPPVGDVPGTPDTPPTVIPGTPGTPGYWHPCP"
     gene            complement(626..>1187)
                     /locus_tag="VFU90_11620"
     CDS             complement(626..>1187)
                     /locus_tag="VFU90_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5480478.1"
                     /translation="QTMARPLSGQAAPGASRRCRPVIVIARVTPGNVSTFKDVRLRAL
                     LDTPSAFGSTYAKESELSDAEWTRRAMRWNGERGIGFLAIDDGIGCGIVGSFLDDDDA
                     ASATLVSMWTAPTHRRQGIARQLVDEVMVWARAHDVKQMRLMVTSSNAAAIAFYEQLG
                     FAGTGRTEPYPNDASVVELEMLRGLA"
BASE COUNT          192 a          426 c          382 g          187 t
ORIGIN      
        1 ggcacctacg tgctggccat catcgacacc gtgtcgcgcg tcggctggat ccatcgcagt
       61 ctcgcgttcc agttgcacgt cgtgtcgatg gtctttacga acggctacgt cgcaacggtt
      121 cgcgagccgg cgcacgcgca acctcgcgat ccgagtgagt taggcccggg gcagcgtccg
      181 gctgcactca tggtggccag tggagtcgcg cccgtcgccg gcctcgcgat cggggcaatc
      241 acgggcggaa aggacggcgc tttgttaggc ggttcgatcg ggagcgccgt cggtttcgtg
      301 acgctcctcg tcgccgcagc gcacagcggc gagtatgatc tgggccccgg cttcccgctg
      361 gagctccgct cccaaggccc gatcgtgctc gacgcaacgc gcaccgcgga ggccgcgcga
      421 atgccatcgc gggtgcagca tcacgagaac acgagctgct acgcgccggg cacacccggg
      481 acgccggaca tcaccattcc gggaacgccg ggcacaccgc cggtgggtga cgtgcccggc
      541 acgccggata cgccgcccac ggtcatcccc ggcacgccgg gaacgccggg ctactggcac
      601 ccctgtccct gagcggcccg ttccgttagg cgagcccgcg gagcatctcg agctcgacca
      661 cggacgcgtc gttcggatac ggttccgttc ggccggtacc ggcaaagcct aactgctcgt
      721 aaaacgcgat ggcggccgcg ttgctcgacg tgaccatcaa gcgcatctgc ttcacgtcgt
      781 gcgcacgcgc ccacaccatc acctcatcga ccagctgccg cgcaatcccc tgccggcgat
      841 gcgtcggagc ggtccacatc gagacgagcg tcgccgacgc cgcatcatcg tcatccagga
      901 acgagcccac gatgccgcaa ccgatcccgt cgtcgatcgc gagaaacccg atgccgcgtt
      961 caccgttcca ccgcatggcg cgtcgagtcc actcggcgtc gctcagctcg ctctctttcg
     1021 cgtacgtcga gccgaaggcg ctcggcgtgt cgagcagggc gcgcagtcgg acatccttga
     1081 acgtcgacac gttgcctggc gtgacgcgcg caatgacgat caccggccgg caccgccggc
     1141 tggcaccggg tgcagcctga cctgaaagag gacgggccat tgtttgc
//
LOCUS       DASUCJ010000978         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_484191, whole genome shotgun sequence.
ACCESSION   DASUCJ010000978 DASUCJ010000000
VERSION     DASUCJ010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484191"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1147
                     /locus_tag="VFU90_11625"
     CDS             <1..>1147
                     /locus_tag="VFU90_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480479.1"
                     /translation="QVSACYILSIEDDLVNEGGIFDGVLREARIFKGGSGSGANFSRL
                     RGAGEKLTGGGTSSGLMSFLKIFDRAAGAIKSGGTTRRAAKMVVLDADHPDIEDFVNW
                     KVREERKVADLVVGSRIFEKHVNAIIAAAHDTRVPEHARLDPSLNAPLRAAIREALAV
                     GIPTGATQNALDYARQGYTHLEVERYDTGWDSEAYVTVSGQNSNNSVRLTNGFFGALD
                     RDEEWHLTARTTGDTVKRLKAAELWEQIGLAAWQCADPGLQFDDTIQEWHTCANDERI
                     NATNPCVTGDTLVATADGPQRIESLVGKAAFVIGSDGAPHFVNRIFPTGTKPVYRLTT
                     KSGYTLKVTTDHKVWTENRGDIPACELLAGDRLALVGSGFGRESLEPR"
BASE COUNT          225 a          392 c          345 g          185 t
ORIGIN      
        1 cgcaggtttc ggcgtgctac atcctatcga tcgaagacga tctcgtcaac gaaggcggca
       61 tcttcgacgg agtgctgcgc gaagcgcgca ttttcaaagg cggctcggga tccggcgcca
      121 atttctcgcg gctgcgcggc gccggtgaaa agttgaccgg cggcggcacc agctccggct
      181 tgatgtcgtt tctgaagatc ttcgaccgcg ccgccggcgc cattaaatcg ggcggcacca
      241 cgcgccgcgc cgctaagatg gtggtgctcg acgccgatca ccccgacatc gaagatttcg
      301 tcaattggaa ggtccgtgag gagcgcaagg tcgcggatct ggtggtcggc tcgcgcatct
      361 tcgaaaagca cgtcaacgcg atcatcgccg cggcccacga tacgcgcgtt cccgagcacg
      421 cccggctgga tccgtcgctc aacgcgccgc tgcgcgcggc gattcgcgaa gcgctagcgg
      481 tcggcatccc gaccggcgcc acccaaaacg cgctcgacta cgcgcgccaa ggctacaccc
      541 acctggaggt cgagcgctac gacaccggct gggactccga agcctacgtc accgtatcgg
      601 ggcagaattc gaataactcc gtgcgcctca ccaacgggtt cttcggcgca ctcgatcgcg
      661 acgaagagtg gcacctcacc gcacgcacga ccggcgatac cgtcaagcgc ctcaaagccg
      721 ccgagctctg ggagcaaatc ggcctggccg cgtggcagtg tgcggacccc gggctgcagt
      781 tcgacgacac gattcaagag tggcacacct gcgccaacga cgagcgcatc aacgcaacta
      841 acccatgcgt taccggcgat acgctggtcg ccaccgccga cgggccacag cgcatcgaga
      901 gcctcgtcgg taaggccgcc ttcgtcatcg gctccgatgg cgctccgcat ttcgtcaacc
      961 gcatcttccc gaccggcacc aagccggtct accggctcac gacgaaatcc ggctatacgt
     1021 taaaggttac taccgatcac aaggtctgga cggaaaaccg cggagatatc ccggcttgcg
     1081 aattgcttgc cggcgatcgc ctcgcgctcg tcggatccgg attcggacgc gaaagcctag
     1141 agccccg
//
LOCUS       DASUCJ010000979         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_422957, whole genome shotgun sequence.
ACCESSION   DASUCJ010000979 DASUCJ010000000
VERSION     DASUCJ010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422957"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="xrtA"
                     /locus_tag="VFU90_11630"
     CDS             complement(<1..885)
                     /gene="xrtA"
                     /locus_tag="VFU90_11630"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEU5480480.1"
                     /translation="MKRDALPGLGTSVGSPRVSTPAASARAAPGWPRALVALGVVLAV
                     TVAAYFGTAAGMVAIWARSETFAHGFVVVPIALWLVWRMRSRLREIEPQPSWLPLPFM
                     AGAGFAWLAGEVGAVNALTQFAFVAMLVLAVPATLGLRVARAMLFPLGFLFFAVPFGE
                     FLLPTLMAYTADFTIAAVRLTGVPVLREGLQVFSVPNGRWSVVEACSGVRYLIASVVV
                     GTLFAYLNYRTMRRRLAFVGVSIAVPIAANWIRAYMIVMLGYLSDNKIATGVDHIIYG
                     WLFFGLVMLIMFWIGSYWR"
     gene            complement(891..>1180)
                     /locus_tag="VFU90_11635"
     CDS             complement(891..>1180)
                     /locus_tag="VFU90_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480481.1"
                     /translation="ANDFKYWELMRRACERGLRLFDYGRSKRGTGSFDFKKNWGFDPA
                     PLDYEYCLLDGSEVPQNNPMNPRFRALIATWRHLPRPLVNAIGPWLARNLG"
BASE COUNT          247 a          410 c          381 g          142 t
ORIGIN      
        1 gcgccaatac gaaccgatcc agaacatgat cagcatcacg aggccgaaga acagccagcc
       61 gtagatgatg tgatcgacgc cggtcgcaat cttgttgtcc gaaaggtagc cgagcatcac
      121 gatcatgtag gcgcgaatcc agttcgcggc aatcggcacc gcgatcgata cgccgacgaa
      181 cgcgagcctg cgccgcatcg tccggtagtt gagatacgcg aacagggtgc cgacgacgac
      241 cgacgcgatc aggtaacgca cgccactgca cgcctcgacc accgaccagc gaccgttcgg
      301 aaccgagaac acctgcaggc cttcgcgcag taccggcacc ccggtgaggc gcaccgcggc
      361 gatcgtgaag tccgccgtgt aggccatcag cgtcggcagc agaaattcgc cgaacggcac
      421 cgcgaagaac aggaaaccca gcgggaacag catcgcgcgc gcgacacgca ggccgagggt
      481 cgccgggacc gcgagcacca gcatcgcgac gaacgcgaac tgcgtaagcg cgttgacggc
      541 acccacctcg ccggcgagcc acgcgaatcc cgcgccagcc atgaatggaa gcgggagcca
      601 ggacggctgc ggctcgatct ctctgagccg cgagcgcatc cgccagacga gccacagcgc
      661 gatcggcacg acgacgaatc cgtgcgcgaa cgtctcggag cgcgcccaga tcgcgaccat
      721 cccggcggcc gtgccgaaat acgccgcgac ggtcaccgca agcacgacac cgagggcgac
      781 gagcgcccgc ggccaacccg gcgccgctcg cgccgaagcc gccggcgtcg agacccgcgg
      841 agagccgacc gacgtgccga ggcccggaag agcgtcgcgc ttcatcgtcg tcagccgagg
      901 ttgcgcgcga gccatggccc gatcgcattg acgagcggcc gcggcagatg gcgccacgtg
      961 gcgatcagcg cacggaacct gggattcatc ggattgttct gcggcacctc gctcccgtcg
     1021 agcaggcagt actcgtagtc gagcggcgcc ggatcgaaac cccagttctt cttgaagtcg
     1081 aacgagccgg tgccgcgctt gctgcgtccg tagtcgaaca gacgcagccc gcgctcacac
     1141 gcgcggcgca tcagctccca gtacttgaag tcgttggcgg
//
LOCUS       DASUCJ010000980         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1309305, whole genome shotgun sequence.
ACCESSION   DASUCJ010000980 DASUCJ010000000
VERSION     DASUCJ010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309305"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFU90_11640"
     CDS             complement(<1..453)
                     /locus_tag="VFU90_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5480482.1"
                     /translation="MLPELDRASIVPLYRQIYEQLREAILAGRLPESARLPPERSLAE
                     KLDVNRSTVVHAYRELATDGLIEQRVGSGSRVVPQLRGGQPERSAGVPWWVTLPPWRV
                     GAFPNVLGELAAEQETGRISFVQGVAPDQPSPLAGLAKSFARVGGEPRF"
     gene            622..>1116
                     /locus_tag="VFU90_11645"
     CDS             622..>1116
                     /locus_tag="VFU90_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480483.1"
                     /translation="MAEHQVDAVVIGAGPGGYHAAIRLGQLGKSVICIDRDEVGGVCL
                     NWGCIPTKALLHVGEVIRHIEQAGAIGLNVPKAAVDRAGVAKLRTEVVNANVGGVKTL
                     FKANGVQFLYGEASFKSPTEIAVKKRDGETDTIRAADVVIATGSAPIDVAAWPRDGDT
                     IINSD"
BASE COUNT          208 a          371 c          383 g          154 t
ORIGIN      
        1 gaagcgcggc tcgccgccga cgcgcgcgaa cgatttggcg agccccgcca gcggcgaggg
       61 ctgatccggc gcgacgccct gcacgaacga tatccggccg gtctcctgtt cggcggccag
      121 ctctccgagg acgtttggaa acgcgccgac ccgccagggc ggcagcgtca cccaccacgg
      181 gacgccggcg gaacgctcgg gctgcccgcc gcgcaactgc gggaccacac gcgaacccga
      241 gccgacgcgc tgttcgatca ggccgtcggt ggcgagctcc cggtaagcgt gcaccacggt
      301 gctgcgattg acgtcgagct tctccgcgag gctgcgctcg gggggcaaac gggccgactc
      361 cggaagccgt ccggcgagaa tcgcctcacg gagctgttcg tagatctggc ggtagagggg
      421 aacgatggac gcgcggtcga gttccggcag catgaaggca cccaagggtc aagatggcgg
      481 ggtccatttt tgacggccgc gggccgcacg cctgcgttcg gcgctcgacg cgaaagtaag
      541 ctattttcgg ccgccgacga ggcgaccgga gggagcgaag ccgaactcca acggccccga
      601 cgtaaagaac aggagctagt tttggccgaa catcaagtcg acgcggtcgt catcggcgcc
      661 ggaccgggtg gatatcacgc cgcgatccgg ctggggcaac tcggaaagag cgtcatctgc
      721 atcgaccgcg acgaggtcgg cggcgtctgc ctcaactggg gctgcattcc caccaaagcg
      781 ctgctgcacg tcggcgaggt aatccgtcac atcgagcagg cgggcgcgat cgggctgaac
      841 gttccgaaag ccgcggtcga tcgcgcgggc gtcgcgaagt tgcgaaccga ggtcgtcaac
      901 gccaacgtcg gcggcgtcaa gaccctcttc aaagccaacg gcgtccagtt tttatacggc
      961 gaggcgtcgt tcaagagccc caccgagatc gccgtcaaga agcgcgacgg cgaaaccgac
     1021 acgatccgcg cggccgacgt cgtgattgca accggctccg ctccgatcga cgtcgccgcg
     1081 tggccgcgcg acggcgacac gatcatcaac tccgac
//
LOCUS       DASUCJ010000981         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1416524, whole genome shotgun sequence.
ACCESSION   DASUCJ010000981 DASUCJ010000000
VERSION     DASUCJ010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416524"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1172
                     /locus_tag="VFU90_11650"
     CDS             <1..>1172
                     /locus_tag="VFU90_11650"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU5480484.1"
                     /translation="MKLVLIPSGEFLMGSAEDDPAERDDEQPRHRVRITLPFYLGAYE
                     VTQAEFENVMATNPSSFSRAGLLKDAPDDLDASKLPVDNVTWHAAIEFCRRLSERPSE
                     KQAGRIYRLPTEAEWEYACRAGTTTAFHYGDSLTSSQANFNGEHPFGDDAKGPFLNRT
                     AIVGSYEPNAFGLYDMHGNLNEWCLDRFERDYYNRSPSDDPPGPLEGASRVIRGGDWY
                     SDGRDCRSAFRYADIPDGRFYALGMRIVCELSSQGATLHPLVATSGRQVKKPISAAEA
                     SPRDEAPKPETGEDWPQWRGPRGDGTWRAPKLPAAWPAGGLTRAWRRELGGGYGGVAV
                     AGVRVYVMDRQREPHDVERVLCFDAASGEPIWSQNYPVDYSAIAYDNGPRATPTIF"
BASE COUNT          210 a          391 c          387 g          184 t
ORIGIN      
        1 gcatgaagct ggtgttgatt ccttcgggcg agtttctgat gggttcggcc gaggacgatc
       61 cggcggagcg cgacgacgaa cagccgcggc atcgcgtacg aatcacgctg ccgttttacc
      121 tcggcgccta cgaggtgacg caagcggagt tcgaaaacgt gatggccacg aaccccagct
      181 cgttcagtcg cgccggcctg ctgaaagacg ctcccgacga cttggacgcc tcgaagctgc
      241 ccgtcgacaa cgtcacgtgg catgccgcga tcgaattctg ccgccggctg tcggagcggc
      301 cgagcgagaa acaagccggc cgcatctacc ggctgccgac cgaagcggaa tgggaatacg
      361 cctgccgcgc gggaacgacc acggcctttc actacggcga ttcgcttacg tcgtcgcagg
      421 cgaacttcaa cggagagcat cccttcggcg acgacgccaa agggccgttt ctgaaccgca
      481 cggcgatcgt cggctcatac gagccgaacg ccttcggcct ctacgacatg cacggcaatc
      541 tcaacgaatg gtgcctcgac cgcttcgagc gcgactatta caaccgctcg ccaagcgacg
      601 atccgcccgg gcccttggaa ggcgcgtcgc gagtgattcg cggcggcgat tggtacagcg
      661 atgggcgcga ttgccgcagc gcatttcgct acgccgacat ccccgacggc cggttttacg
      721 ctttgggcat gcgcatcgtt tgcgagctgt cctcgcaagg ggcgacgctc catccgcttg
      781 tcgcgacgtc gggccggcaa gtcaagaagc caatctccgc cgcggaggct tcgccgcgcg
      841 acgaagcgcc gaagcccgag acgggcgaag actggcccca gtggcgcggc ccgcgcggcg
      901 atggaacctg gcgtgcgccg aaattgccgg ccgcctggcc agccggcggg ctcacgcgcg
      961 cttggcggcg agagctcggc ggcggttacg gcggagtggc ggttgccgga gtccgggtct
     1021 acgtgatgga ccggcagcgc gagccgcacg acgtcgaacg cgtgctctgc ttcgacgccg
     1081 ccagcggcga accgatctgg tcgcagaact acccggtcga ttacagcgcc atcgcctacg
     1141 ataacggccc gcgtgcgacg ccgaccatat tc
//
LOCUS       DASUCJ010000982         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_988571, whole genome shotgun sequence.
ACCESSION   DASUCJ010000982 DASUCJ010000000
VERSION     DASUCJ010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988571"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VFU90_11655"
     CDS             <1..1372
                     /locus_tag="VFU90_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU5480485.1"
                     /translation="SPALAAIVNRLLQKEPGHRFASASDLATALREARERPSVAGSRI
                     ADDSPTQVRRTVRSLPPRRSPLPDRPYDEDGGDAPAGRRSPWVAVALIAVLLIATGIG
                     YYLFGRPLPSLDATIAVGDYTNMTDAEAQQTVVNAGLRVRFIKSPSDTIVANHVIRQN
                     PLAGTKVAKDSLVELVISNGKPLVGLTDVRGYSVQDAQRTLQGLGFAVTIARKFDTSP
                     KDNVVDQQPKAGTKVRQGSRITLMVSDGPQPVFVPNFVGLSVDKAKALAAKMGLTIDT
                     SQQAAITGVPADTIASQNPAPGAQTQKGGAVFAVVSTGVQNVPAQPVGAAPVAVPSVI
                     GRPYQEAIDAITGAGFRAAVQFQVLTSNNGTAVAEDPAAETQLPAGSTVTLTISVPGE
                     IPDTEGMAVDKARAALAAAGYVVGRVQYTPTGVGGRVVGTTPSVGTPLPPGSAVTLVV
                     NAGPPQ"
     gene            1384..1905
                     /gene="ruvC"
                     /locus_tag="VFU90_11660"
     CDS             1384..1905
                     /gene="ruvC"
                     /locus_tag="VFU90_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5480486.1"
                     /translation="MRILGIDPALRTTGYGVIESSSGRVRLVEAGVVAPRAGAALEQR
                     LRELHAGIAEVIAQTQPQSVVIEELYTVYRNPSTAILMAHARGVLCLAAAQAGIGVNT
                     LGHAHVKRALVGSGSARKGQVNAMVTQLLGLRVAPEPEDVSDALALALAFLNMSHPRT
                     SAARLARRARMVR"
     gene            1905..2540
                     /gene="ruvA"
                     /locus_tag="VFU90_11665"
     CDS             1905..2540
                     /gene="ruvA"
                     /locus_tag="VFU90_11665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5480487.1"
                     /translation="MPFSRIAGTLVERIGDAVVIDTGGLGYEIVVPPCVAEKLPTKPG
                     SPLTLEVYAVVNLDGNSGRFSYYGFTNAIEREFFEALISVASIGPRSAARAFSQPMSS
                     IARAIDRGDYTALKSLPGIGQQKARDIVAKLQGKVAKFLLIQDAPPAPEAEIPDFAAE
                     ALAVLLQLEYKRGEAEAMIRETLEAGPALADAEALLAEIYRRRASKGTSTS"
     gene            2537..3601
                     /gene="ruvB"
                     /locus_tag="VFU90_11670"
     CDS             2537..3601
                     /gene="ruvB"
                     /locus_tag="VFU90_11670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5480488.1"
                     /translation="MSGDSARKRLLGPSDELERVVDPGDTLEDEVYGASLRPRTFDDY
                     VGQDRVVENLRIAIDAAKRRGEPLEHVLFYGPPGLGKTTLAGLIAREIGANFHPTSGP
                     TLEKPKDLVGILTSLEDGDILFIDEIHRLGRVVEEFLYPAMEDFQIDFVVDRGAFAKT
                     LKLPLKRFTLVGATTRAGMLTAPLRERFGIVHHLDYYSAGELERILRRSAAVLGVPID
                     DDGAATIARRSRGTPRIANRLLRRVRDFAEVRAAGRITAAVAGEALEREGVDELGLDR
                     LDRAFLRTLVEQYGGGPAGIAAIAATLTEDAETLEDVVEPYLLKEGFVMRTASGRRAT
                     PEAYRHLGATPRGSVQERLL"
     gene            3624..>4117
                     /locus_tag="VFU90_11675"
     CDS             3624..>4117
                     /locus_tag="VFU90_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5480489.1"
                     /translation="MATVAASALFPRLALAQQSGDPALTSARPALRVLLGTGTASPLP
                     GGGFTYDGRTFRGTFEYSSDGGVVNLVDLEQYLYAVVPHEMPSSWPPAALQAQAVCAR
                     TYVLQRSDPRRGYDVMPSEIDQVYPGVAGETPAGRLAVDATAGRVLRYASGFASIAYS
                     SCCGG"
BASE COUNT          671 a         1382 c         1429 g          635 t
ORIGIN      
        1 cagcccggcc ctcgcggcaa tcgtcaaccg cctgctgcaa aaggagcccg ggcaccgctt
       61 cgcgtcggcg agcgacctgg caaccgccct gcgcgaagcg cgcgagcgtc cgagcgtggc
      121 cggctcgcgg atcgccgacg actcgccgac gcaagtacgg cggacggttc gatcgctgcc
      181 gccgcggcgt tcgcccttgc cggaccgccc ctacgacgaa gacggcggcg acgcgcccgc
      241 cgggcggcgg agcccgtggg tcgcggtcgc attaatcgcc gtgctgctga tcgcaaccgg
      301 aatcggctac tatttgttcg gccgtccgct gccctcgctc gacgcgacga tcgcggtcgg
      361 cgactacacg aacatgaccg atgccgaagc ccaacagacg gtggtcaacg cggggttgcg
      421 cgtgcgtttc atcaagagcc ccagcgacac gatcgtcgcg aatcacgtga ttcgccaaaa
      481 tccgctggcc ggcacgaagg tggcgaagga ttcgctggtc gagctggtga tcagcaacgg
      541 caaaccgctg gtcggcctga ccgacgtgcg cggctattcg gtgcaggacg cgcagcgcac
      601 gctgcaaggc ctcggatttg cggtgacgat cgcgcgcaag ttcgatacga gtccgaagga
      661 caacgtcgtc gatcagcagc cgaaagccgg cacgaaggtg cgccagggca gccgtatcac
      721 gctgatggtg tcggatggtc cgcagccggt cttcgtgccg aacttcgtgg gcctctccgt
      781 cgacaaggcg aaggcactcg ccgccaagat gggtttgacg atcgacacct cccagcaagc
      841 ggcgatcacc ggcgtgccgg ccgatacgat tgcgtcgcag aaccccgcgc ccggagcgca
      901 aacgcaaaag ggcggggcgg tgttcgcggt ggtgagcacc ggcgtgcaga acgtgccggc
      961 gcagccggtc ggggcggcgc cggtggcggt gccctcggtg atcggacggc cgtatcagga
     1021 agcaatcgac gcgattaccg gtgcgggctt ccgcgcggcg gtgcaatttc aggtgctgac
     1081 gagcaacaac ggtaccgcgg tcgccgagga tccggcggcc gagacccagc tgccggcggg
     1141 ttccaccgtc acgctcacga tttcggtccc cggtgaaatt cccgataccg aagggatggc
     1201 ggtcgacaaa gcgcgcgccg ccctggcggc ggcgggctac gtggtcggac gcgtgcagta
     1261 cacgcccacc ggcgtgggcg ggcgagtcgt gggaacgact ccgtcggtcg gaacgccgct
     1321 accgcccggc tccgcggtga cgctggtcgt caacgccggc ccgccgcaat aagcgtttcg
     1381 ccgatgcgca tcctcggaat cgacccggcg ctgcggacca ccgggtacgg cgtcatcgag
     1441 agctcgtccg gccgcgtgcg gctggtggaa gcaggcgtag tcgcgccgcg agccggcgcc
     1501 gcactcgagc agcggctgcg cgagcttcac gccgggattg cggaggtgat cgctcagacc
     1561 caaccgcagt cggtcgtcat cgaagagctg tacaccgtct atcgcaatcc gtcgacggcg
     1621 atcttgatgg cgcacgctcg cggcgtgctg tgtctggcgg cagcgcaagc cgggatcggc
     1681 gtcaacacgc tgggccacgc gcacgtcaag cgtgcgctgg tgggctcggg aagcgcgcgc
     1741 aaggggcagg tcaacgcgat ggtcacgcag ctgctcggat tgcgcgtcgc gccggaaccc
     1801 gaagacgtct ccgacgcgct cgcgctggcc ctggcctttc tcaacatgtc gcacccgcgg
     1861 acctctgcag cccgtctcgc gcggcgcgcc cggatggtcc gctagtgccg ttctcacgga
     1921 tcgccggaac gctggtggag cgcatcggcg acgcagtggt gatcgacacc ggcggcctcg
     1981 ggtacgaaat cgtcgttccg ccgtgcgtcg ccgaaaaact tccaaccaag cccggcagcc
     2041 cgctgaccct cgaagtctac gcggtcgtca acctggacgg gaattccgga cgtttttcgt
     2101 attacgggtt caccaacgcg atcgagcgcg aattcttcga agcgctgatc agcgttgcgt
     2161 cgatcggtcc gcgcagcgcc gcgcgcgcgt tttcccagcc gatgtcgtcg atcgcgcggg
     2221 cgatcgatcg cggcgactac accgcactca agtcgctgcc ggggatcggg cagcaaaagg
     2281 cgcgcgacat cgtcgcgaag ctccagggga aagtggcaaa gttcttgctg atccaagatg
     2341 ccccgcccgc ccccgaggcc gagattccgg atttcgcggc cgaagcgctc gccgtgttgc
     2401 tccagttgga atataagcgc ggcgaagccg aggcgatgat tcgcgaaacg ctcgaagccg
     2461 gcccggccct cgccgacgcc gaagcgctgc tggcggagat ataccggcgg cgtgcgtcga
     2521 aaggcacgag tacttcatga gcggcgacag cgcgcgcaag cgccttctcg gcccgagcga
     2581 cgaactcgag cgcgtcgtcg accccggcga cacgctcgaa gacgaagtct atggggcgag
     2641 cctgcgtccg cggaccttcg acgattacgt cggccaggat cgcgtggtcg agaacctgcg
     2701 catcgcaatc gacgcggcca agcgccgcgg cgagccgctc gagcacgtgc tgttctacgg
     2761 gccgcccggg ctcggaaaga cgaccctggc cgggctgatc gcgcgcgaga tcggcgcgaa
     2821 ttttcatccg accagcggtc cgacgctcga aaaacccaag gacctcgtcg gcattctcac
     2881 ctcgctggaa gacggcgaca tcttgttcat cgacgaaatc caccggctcg gacgcgtcgt
     2941 cgaagagttt ctctatccgg cgatggagga cttccagatc gacttcgtgg tcgaccgggg
     3001 cgcgtttgcg aaaacgctca aactgccgtt gaaacgcttc acgctggtcg gcgcgacgac
     3061 gcgcgcgggg atgctgacgg cgccgctgcg ggagcgcttc gggatcgtgc atcatctcga
     3121 ttattactcg gccggcgagc tggagcggat tctgcgccgt tcggcggccg tgctcggcgt
     3181 gccgatcgac gacgacgggg cggctaccat tgcgcgccgc agccgcggca ccccgcgcat
     3241 cgccaatcgc ctgctgcggc gcgtccgcga tttcgccgaa gtacgcgccg ccggccggat
     3301 caccgcagcg gtagccggcg aagcgctcga acgcgaaggc gtcgacgaac tcgggctgga
     3361 tcgtttggat cgcgcgttcc tgcgcacgct ggtcgaacaa tacggcggcg ggccggcggg
     3421 catcgcggcc atcgccgcga ccctcaccga ggacgccgaa acgctggaag acgtcgtcga
     3481 gccgtatttg ctcaaggaag gttttgtgat gcgcaccgcg tcgggccggc gcgcgacgcc
     3541 cgaagcctac cggcacctcg gcgcgacgcc gcgcggttcg gtccaggagc gcttgttgta
     3601 ggatgccgaa ccggcgaacg ttcgtggcga cggttgcggc cagcgcgctc tttccgcgcc
     3661 tcgcgctcgc gcagcaatcc ggcgatccgg ccctgacgag cgccaggccg gcgctgcgcg
     3721 tgctgcttgg gaccggtacc gcgtcgccgc tgccgggcgg cggattcacc tacgacgggc
     3781 gcacgttccg cggtacgttc gagtactcga gcgatggcgg cgtcgtcaac ctcgtcgact
     3841 tggaacagta tctctacgcg gtcgtgccgc acgagatgcc gtcctcgtgg ccgccggcgg
     3901 cgctgcaggc gcaagccgtc tgcgcgcgga cgtacgtcct gcaacgcagc gatccgcgcc
     3961 gcgggtacga cgtgatgccg tcggagatcg atcaagttta tcccggcgtc gccggtgaga
     4021 cgccggccgg acggctggcg gtcgacgcca ctgccggccg ggtgctgcgt tatgcgagcg
     4081 gcttcgcgtc gatcgcgtac tcgtcgtgct gcggcgg
//
LOCUS       DASUCJ010000983         1213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1431613, whole genome shotgun sequence.
ACCESSION   DASUCJ010000983 DASUCJ010000000
VERSION     DASUCJ010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431613"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFU90_11680"
     CDS             complement(<1..703)
                     /locus_tag="VFU90_11680"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEU5480490.1"
                     /translation="MQRRLSRRRLSVALDVRHHAPCLEPRRACALPLIAAALAAAVVS
                     GAAAAQGNPGTTRDSIADRPITAKTVDRAPPMTGRMDAPPWDAADSITDFRQKDPFEG
                     TPASERTVVKVLHDADALYVAVRAWDRDATHIRSTQLRRDADLSSDDHVTILIDSFHD
                     RRSAYEFLTNPNGARVDIQITTQDENPNWNGIWDVATRRDSAGWTAVFRIPFRTLRFK
                     SGAGTSFGFNVKRYIR"
     gene            complement(825..>1213)
                     /locus_tag="VFU90_11685"
     CDS             complement(825..>1213)
                     /locus_tag="VFU90_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480491.1"
                     /translation="GGLTIAARAGAQKIWGPYPAFEAAFIGGEGTVAGLPPQRYAGDA
                     STYGTVEARVRLITLPFVLRWDFGVSGIVDAGRVYVSGEKSNTWHRGIGGGVWFLLPA
                     RTFGLILDVVGSEGNTRVYGGTRFSY"
BASE COUNT          203 a          382 c          419 g          209 t
ORIGIN      
        1 gacggatgta gcgtttgacg ttgaatccga acgacgtgcc ggcgccggac ttgaagcgca
       61 gcgtgcgaaa cggaatgcga aacaccgccg tccatccggc cgaatcacgc ctcgtcgcga
      121 catcccatat gccgttccaa ttcgggtttt catcttgcgt cgtgatttga atgtcgacgc
      181 gcgcgccgtt ggggttggtg aggaactcgt acgcactgcg ccggtcgtga aagctgtcga
      241 tgagaatcgt gacgtgatca tcgctcgaca ggtcggcgtc gcgtcggagc tgagtggacc
      301 tgatgtgggt cgcgtcgcga tcccaggcgc ggacggcgac gtagagtgca tcggcgtcat
      361 gcaggacttt gacgacggtt cgctcgctcg ctggcgtgcc ctcgaacgga tctttctggc
      421 ggaaatcggt gatggagtcg gcggcgtccc agggcggggc atccatgcgg ccggtcatgg
      481 gcggcgctcg gtcaacggtc ttcgcggtga tcgggcggtc ggcgatcgag tcgcgggtcg
      541 tgcccgggtt gccttgggca gcagccgcgc cggagacgac agctgcggcg agcgccgcgg
      601 cgatcaacgg caacgcgcac gcgcgtcgag gctcgagaca gggggcatga tgcctaacgt
      661 cgagcgcgac cgacagccgc cggcgactca gtcgtcgttg caacggagct cctctccagc
      721 ggttgggttc gctgccaccg ccctggaagt accgcatctc gtggttaggc gccaggggag
      781 gaagttgaga tggagtgcac gccttcagtc gctgctgagc gcgctcagta cgagaatcgc
      841 gtaccgccgt acactctggt gttgccctcg ctgccgacca cgtcgaggat caggccgaac
      901 gtgcgcgcgg gaagcaagaa ccacacgcca ccaccgattc cccgatgcca tgtgttcgat
      961 ttctccccgc tcacgtacac tcggccggca tccacgatgc cgctcacgcc gaagtcccaa
     1021 cgcagcacaa agggcagggt gatgaggcgc acgcgtgcct cgacggtgcc gtaggtcgag
     1081 gcgtcaccgg cgtaccgctg cggcggcagc ccggcgacgg tgccttctcc gccaatgaac
     1141 gccgcctcga acgccggata ggggccccag attttctgcg cgccggcgcg cgcggcaatc
     1201 gttaggccgc cgg
//
LOCUS       DASUCJ010000984         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_866367, whole genome shotgun sequence.
ACCESSION   DASUCJ010000984 DASUCJ010000000
VERSION     DASUCJ010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866367"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1191
                     /locus_tag="VFU90_11690"
     CDS             <1..>1191
                     /locus_tag="VFU90_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEU5480492.1"
                     /translation="DGGIFPSQTLTLTVERLQWNARERRYETTDRQVQTLVTDAAGSA
                     TFAWVPPVAATYKFALQGTDEHRRVATASAFTVAATAESAGDNALPDRWLRLVPEKTT
                     LAPGEHARVLVMAPVANRDALVAVCTDRLVRLRLVHISGTAAVIDEAPPPNAAKFSVS
                     VLQGGESGGLSGIARLSVDPPPRRLVVRIVPSKRRYEPGETAGLRIDVRDSAGRPARA
                     EVGIGVVDEAIYALQEERQSPFDAFYGYAPGPFAIGQWNMTVQPYRNFTLRTIANVSA
                     RAAMPPPTASASSDSASVRVRQNFLDTAFWAPSVLTDERGHAEVSFPWPDDLTTWRAT
                     GTAVTTATGIGTGTADALVTKDFLVRLATPRFLRLGDRSTLTGIAQGVTAAPAVTLRL
                     EADA"
BASE COUNT          195 a          382 c          430 g          184 t
ORIGIN      
        1 acgacggcgg catctttccc agccaaacgc tgacgctgac ggtcgaacgt ctgcagtgga
       61 acgcgcgcga gcggcgctac gaaacgaccg atcgacaggt gcaaacgctc gtgaccgacg
      121 cggcgggaag tgcgaccttt gcgtgggttc ctccggtagc ggcgacgtac aagttcgccc
      181 tccaggggac ggacgagcac agacgggtcg ccacggcgtc ggcgtttacc gtggcggcaa
      241 ccgccgagag tgccggcgac aacgcgctcc cggatcggtg gctcaggctc gttcccgaaa
      301 aaacgacgct cgcgcccggc gaacacgcgc gcgtgctggt gatggcgccg gtcgccaacc
      361 gcgacgcgct cgttgcggtc tgcacggatc ggctggtgcg gctgcggctc gtgcacatca
      421 gcggcacggc tgcagtgatc gacgaagcgc cgccgccgaa tgcagccaag tttagcgtca
      481 gcgtcctgca gggcggcgaa tcgggcggcc tgagcggcat tgcgaggttg tcggtggatc
      541 cgccgccgcg ccggctggtc gtgcgtattg tgccgagcaa gcggcgctac gagccgggtg
      601 aaaccgcggg tttacgcatc gacgtgcgcg attcggccgg ccggccggcg cgcgcggagg
      661 tcggaatcgg ggtcgtcgac gaggcgattt acgcgctgca ggaggagcgt cagagtccgt
      721 tcgacgcgtt ttacggctac gcgcccgggc cgttcgcgat cggtcaatgg aatatgaccg
      781 tgcagcctta tcgcaacttc acgttgcgga ccatcgcaaa cgtctccgcg cgggcggcca
      841 tgccgccgcc gacggcgagc gcatcgagcg actcggcaag cgttcgggta cgccagaatt
      901 tcctggacac ggccttctgg gcgccgtcgg tactcacgga cgaacgcggt catgcggaag
      961 tatcctttcc gtggcccgac gatctcacga catggcgtgc gaccgggacg gcggttacga
     1021 ccgcgaccgg tatcggaacg ggcacggccg acgcgctcgt cacgaaagac tttctggtgc
     1081 gcctggcgac gccgcgattc ttgcgcctgg gcgaccgctc gacgctgacc ggaatcgcgc
     1141 agggcgtgac ggcggctccg gcggtaacgt tgcggctgga ggcagacgcg c
//
LOCUS       DASUCJ010000985         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_331034, whole genome shotgun sequence.
ACCESSION   DASUCJ010000985 DASUCJ010000000
VERSION     DASUCJ010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331034"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFU90_11695"
     CDS             complement(<1..906)
                     /locus_tag="VFU90_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5480493.1"
                     /translation="MTLLFVAAAFVLGAAAGALVAWLASRAETSGLRATLVESQRSQE
                     KTIEALLERAKNELRDATASRASERVGELVAPVAQKLHEFDRLIQELEAKRQHDAGSL
                     RAQIDQLLGRAEKLESATTSLTTQTSTLVTALRNPASRGKWGEMQLRNVVEKAGMLPY
                     CDFSEQQTVALEEARVRPDMTVNLPGERFVFVDAKAPTDAMQAALECADDQQRRALVK
                     QHSRALREHVDALARRGYQTAKGSADYVVMFVPGEAFLSAACNDDPALIEYALDHNVL
                     VAGPLSLISLLRTFAMGWQALRQEEN"
     gene            complement(903..1757)
                     /locus_tag="VFU90_11700"
     CDS             complement(903..1757)
                     /locus_tag="VFU90_11700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5480494.1"
                     /translation="MDPTKATAPELNRRAFVGIAVAAAGAGSSAVAAAQTAPAMGSAH
                     PPLVPENDPSIGVARPRLALPDATVPAYAAWPKDAGDATPSVVVVMHIWGTDTSIRDV
                     VRRFAKAGIAAIAPDLYARFGAPGGDGVADIAVFRPYAKQLERTQYDGDLAAAAAWLK
                     SKFPSTKAGIVGFCMGGRIALLAAIDDRGVFSAVCPFYGPLEGVDPKQLHVPVCGSYG
                     ARDSSIPADSVRAFAAQLTVPNDVRIYDAAGHAFFDDQRPAYVASAAEDAWTRTLAFL
                     RKYAGPES"
     gene            1863..>2528
                     /locus_tag="VFU90_11705"
     CDS             1863..>2528
                     /locus_tag="VFU90_11705"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5480495.1"
                     /translation="MHANDFRVAAHRALEAFRSLSIVAAAIAATACSPARQAAYDPQA
                     LPPGPVGRAIAYGHDIVVDTPHLMKGYVRARMSCEACHLDAGTKPKGGSFAGTYARFP
                     QWNRRAHRVIALQDRIAECFLYSMNGRPPAYTSKPMIALVAYIAWLSRDTPVGAPQPA
                     SDRYLEPLPQRTPNVRHGLALYVKKCMQCHQADGEGIAGAFPPLWGPTSFNSGAGMAR
                     IDRM"
BASE COUNT          408 a          849 c          853 g          418 t
ORIGIN      
        1 attttcttcc tgacgcagcg cctgccaacc catcgcaaac gtgcgcagca ggctgatgag
       61 cgacagcggc cccgcaacca gcacgttgtg gtcgagcgcg tactcgatca gggcgggatc
      121 gtcgttgcag gcggcgctca ggaaggcttc ccccggcacg aacatcacca cgtaatcggc
      181 ggagcctttc gcggtttggt acccgcggcg cgcgagcgcg tcgacgtgct cgcgcagcgc
      241 acgcgagtgt tgctttacca aagcgcgccg ctgctgatcg tcggcgcact ccagggcggc
      301 ctgcatcgcg tcggtcggcg cctttgcgtc cacgaatacg aaacgttccc ccggtaagtt
      361 caccgtcata tccggacgca cgcgcgcttc ctccagcgcg acggtctgct gttcggagaa
      421 gtcgcaatac ggaagcattc cggctttctc gacgacgttt cgcagctgca tctcgcccca
      481 tttgccgcgc gacgcggggt tgcgcagggc ggtgaccagc gtcgaggttt gggtcgtcaa
      541 gctcgtcgtg gcgctttcca atttttcggc gcgacccaag agctgatcga tctgcgcgcg
      601 caagctgccg gcgtcgtgct gccgcttggc ttcgagttct tggatgagcc ggtcgaactc
      661 gtgcagcttc tgcgcgaccg gagcgacgag ttcgccgacg cgctcgctcg cgcgacttgc
      721 ggtcgcgtcg cgcagctcgt tcttggcgcg ctccaacagg gcctcgatcg tcttctcctg
      781 cgagcgctgc gactcgacga gcgtggcgcg cagtccgctg gtttcggcgc gcgacgcgag
      841 ccatgcgacg agcgcgccgg cggcggcgcc gaggacgaac gcggcggcga cgaaaagaag
      901 cgtcaacttt ccggccccgc gtacttgcgc agaaatgcga gcgttcgcgt ccacgcgtct
      961 tcggccgcgg acgctacgta cgccggccgc tgatcgtcga agaacgcgtg cccggcagcg
     1021 tcgtagatac gaacgtcgtt cggaacggtc agctgggcgg cgaaggcgcg caccgaatcg
     1081 gccgggatgc tgctgtcgcg ggcgccgtaa ctgccgcaca cggggacgtg cagctgcttc
     1141 ggatcgaccc cctcgagcgg cccgtaaaac ggacagacgg ccgagaagac gccgcggtcg
     1201 tcgattgccg ccagcaaagc gatgcgcccg cccatgcaaa aaccgacgat gccggctttg
     1261 gtcgacggaa atttcgattt gagccaagcg gccgccgcgg cgagatcgcc gtcgtattgg
     1321 gtgcgttcga gctgtttggc atacggcctg aaaacggcga tatcggcgac gccgtcgccg
     1381 ccgggtgcgc cgaagcgcgc gtagagatcc ggcgcaatcg ccgcgatccc ggctttggcg
     1441 aaacggcgca cgacgtcgcg aatcgacgta tcggtgcccc agatgtgcat cacgacgacg
     1501 accgacggcg tcgcatcgcc ggcatccttc ggccacgccg cataggcggg gacggttgcg
     1561 tccgggagcg cgagccgcgg acgcgccacg ccgatcgagg ggtcgttctc cggcacgagc
     1621 ggcggatgcg cgctacccat cgcgggtgcc gtttgcgcgg ctgcaacggc cgagcttccc
     1681 gcaccggcgg cggcgacggc gattccgacg aaggcgcgcc ggttgagttc cggagcagtc
     1741 gccttcgtgg gatccacgtc gtacccgctc atagacgttg cctttgacct tcgtacgcgg
     1801 cgcccctagg aaacgcgatt gcgaaagtag tcgggcatcg cgaaggggaa cagcacgacg
     1861 gcatgcacgc caacgatttc cgcgtcgcgg cgcatcgcgc gctcgaggct ttccggtccc
     1921 tgtcgatcgt cgccgccgcg atcgcggcaa cggcgtgttc gccggcacgg caggcggcgt
     1981 acgacccgca ggcgcttccg cccggtccgg tcggccgcgc gatcgcctac ggacacgaca
     2041 tcgtcgtcga tacgccgcac ctcatgaaag gctacgtccg cgcgcgcatg tcgtgcgagg
     2101 cctgtcatct cgacgccggt acgaaaccga agggcggaag ttttgcggga acgtacgcgc
     2161 gctttccgca gtggaaccgg cgcgcgcacc gggtgatcgc cttgcaggac cggatcgccg
     2221 agtgcttcct ctactcgatg aacggccggc cgccggccta cacgagcaaa ccgatgatcg
     2281 cgctggtcgc ctatatcgcg tggctgtcgc gcgatacgcc ggtaggcgcg ccgcagccgg
     2341 cgtcggaccg ctacctcgag ccgctgccgc agcgaacacc gaacgtgcga cacggtctcg
     2401 cgctctacgt caagaagtgc atgcagtgcc accaagccga cggcgaagga attgccggag
     2461 ccttcccgcc gctgtggggt ccgacttcgt ttaattccgg cgccgggatg gcgcggatcg
     2521 atcgtatg
//
LOCUS       DASUCJ010000986         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_117660, whole genome shotgun sequence.
ACCESSION   DASUCJ010000986 DASUCJ010000000
VERSION     DASUCJ010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117660"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VFU90_11710"
     CDS             <1..1619
                     /locus_tag="VFU90_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5480496.1"
                     /translation="PGILWAQTSAGEEITSESVAKAAELAGLEFDDDERAMMVAGLTK
                     QEKQLATLHGISLTNDVVPAVHFTPVLPGTTLATGGHRMVRSTVAPRAVPASHAELAF
                     LPVTELSALVRRRKVTSEQLTRLSLDRLQEFDPKLHCVITLTADRALEHARAADEETR
                     RGRSRGPLHGIPWGAKDLLAVKGYPTTWGSTPFKDQMLPEDATVVRRLDAAGAVLVAK
                     LSLGELAMGDVWFGGMTRNPWKLDQGSSGSSAGPACATAAGLVGFSVGSETLGSISSP
                     STRCGATGLRPTFGRVPRTGAMALSWTMDKLGPICRSVEDCALVLDAIRGSDGHDPTA
                     VDAAFPFDARTTPASLRVGYIKSAFDADHPTKKFDDAALDALREIGAKLVPVELPDQP
                     WGAMTIVLRAEGAAAFDELTRSGRDKEMAQQGPDAWPNTFRVARFIPAVEYVNANRAR
                     TLAMRAIEQTFADVDVVVTPTFGAQLVATNLTGHPAVIVPNGFRDDGTPVSLTFLGRL
                     YGEAPALALARAYQHHTGFHLKHPDWLMTA"
BASE COUNT          282 a          585 c          629 g          280 t
ORIGIN      
        1 tgcccggcat actctgggcg cagacgtcgg ccggcgaaga aatcacgagc gaatcggtcg
       61 ccaaagcagc cgagctcgcc ggactcgagt tcgatgacga cgagcgggcg atgatggttg
      121 ccggcctaac gaaacaagaa aagcagctcg ccaccctgca cggaatctcg ctcaccaacg
      181 acgtcgtgcc ggcggtgcac ttcactccgg tgctgccggg gacgacgctc gcgacgggcg
      241 gacaccgcat ggtgcgctcg acggtggcgc cgcgcgcggt gcccgcctcg cacgccgagc
      301 tcgcctttct gcccgtcacc gagctgtcgg cgctcgtgcg gcggcgcaag gtgacgagcg
      361 agcagctgac gcggttgtcg ctcgatcggc tgcaggagtt cgatccgaaa ctccactgtg
      421 tgatcacgct gacggcggac cgcgccctgg agcacgcccg cgcggccgac gaggaaacgc
      481 gccgcggccg gtcgcgcggg ccgctgcacg ggattccatg gggcgccaag gatttgctgg
      541 cggtgaaagg ttatcccacg acctggggca gcaccccgtt caaggaccag atgcttcccg
      601 aggatgcaac cgtggtgcgc cgcctggacg cggcgggcgc ggtgctcgtc gcgaagcttt
      661 cgttaggcga gctcgccatg ggcgacgtgt ggttcggcgg catgacccgc aacccgtgga
      721 agctggatca aggatcgagc gggtcctcgg cggggccggc gtgcgcgacg gcagccggac
      781 tcgtgggatt ttcggtcggc agtgagacgt tgggctcgat ctcatcgccg tccacgcgct
      841 gtggcgcgac cggcctgcgt ccgacgttcg gacgtgtacc gcgcacgggc gccatggcgc
      901 tctcgtggac gatggacaag ctcggaccga tctgtcgaag cgtcgaggac tgcgcgctgg
      961 tgctcgacgc gattcgtggc agcgacggcc acgatccgac ggcggtggac gcggcgttcc
     1021 cgttcgatgc gcgcaccacg ccggcgtcgc ttcgcgtcgg atacatcaag agcgcgttcg
     1081 atgcggatca cccgacgaag aaattcgatg acgcagcgct ggatgcctta cgcgagatcg
     1141 gcgcgaagct ggtgccggtg gagctgcccg atcagccgtg gggcgccatg acgatcgtgt
     1201 tgcgcgccga aggcgccgcg gcgttcgatg agctcacgcg atcggggcgg gacaaggaga
     1261 tggcccaaca agggccggac gcgtggccga atacgttccg ggtcgcgcgg ttcatccctg
     1321 cggtggagta cgtgaacgcc aaccgggcgc ggacgctggc catgcgggcg atcgagcaga
     1381 cgttcgccga cgtcgatgtg gtggtcacgc cgaccttcgg cgcgcagctc gtggcgacga
     1441 acctgacggg gcacccggcc gtgatcgtgc ctaacggctt ccgcgacgat ggcacgcccg
     1501 tgtcgctgac gttcctcggc cggttgtacg gtgaagcgcc ggctctcgcc ctggcgcgcg
     1561 cctaccagca tcacaccggc ttccatctca agcatcccga ctggttgatg accgcctaac
     1621 gaggtcaggc gccgtcgcgg cgcgcccgca cctcgtctag cacgcgctcg atcttgccct
     1681 cgaggcggtc cagacgctgc gtgatgacgt cgcgccagcc ctcctcattg gtgggatgct
     1741 tcaagacgaa ggtgagcgtc cctcccactt cgagcc
//
LOCUS       DASUCJ010000987         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1370697, whole genome shotgun sequence.
ACCESSION   DASUCJ010000987 DASUCJ010000000
VERSION     DASUCJ010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370697"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFU90_11715"
     CDS             complement(<1..833)
                     /locus_tag="VFU90_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480497.1"
                     /translation="MRFLVCYLLFWTAAFLVARGIFIVYEHHAAAQLGIGTIGRVFLN
                     GARMDLSAAAYATIVPSLLIILGRVVRPSVVRGALATYTYALVVVVTLFTAADLGIYD
                     AWGVRLDATPLTYLRTPQEAYASAESGPVVLCAIVALVFGALCIVAYRRLVARRLDAR
                     PTTTGASAAVLALGAFLIVPIRGGVQWTPLNQSSVYFSSNEFADQAALNVPWNVAHSL
                     TTENALGRANPYTAIDAATARRVVDSLLADPSATPSTLLRIRRPNIILIIWESLTAKV
                     VA"
     gene            1201..>2289
                     /locus_tag="VFU90_11720"
     CDS             1201..>2289
                     /locus_tag="VFU90_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480498.1"
                     /translation="MIAATLATGFAFSLPSTAAAQYFGRNKVQYQTFHFKRLKTPHFD
                     VYYYAAESLAAVDAARMAERWYARHSKTLTDEFDRRSIILYANHADFEQTNVIGGFID
                     QSTGGVTEGLRSRVVLPFTGVYADDDHVIGHELTHVFQYDIAGKMKGGGMIALDRLPL
                     WLIEGMAEYLSLGRNDPNTAMWLRDAALRNDIPTIKKLSTDPRYFPYRYGEALWAYIG
                     GRWGDDVVQQLYRASLQSGFELGIRRTLGMSSDTLSAQWLQAIRDAYLPLMQGRQAPD
                     SLGKRLLRPGSKYGDMDVSPALSPNGKYLAFLTTRGLLNIDLYVADAQTGQIVKKLTG
                     PNSDQHFDAISFINSSGSWSPDGNDFAFI"
BASE COUNT          449 a          734 c          758 g          348 t
ORIGIN      
        1 gccacgacct tggccgtcag actctcccag atgatgagaa tgatgttggg ccggcggatc
       61 cggagcagcg tcgacggcgt ggcggatgga tcggcgagca ggctgtccac gacgcggcgc
      121 gcggtggccg cgtcgatggc cgtgtacgga ttcgcgcggc ccaacgcatt ctcggtggtc
      181 agcgaatgcg cgacgttcca cggcacgttg agcgcggcct gatccgcgaa ttcgttgctc
      241 gagaagtaca cgctgctctg attgagcggc gtccactgca cgccgccgcg aatcggcacg
      301 atgaggaagg cccccagcgc gaggacggcc gccgaggctc cggtcgtggt tgggcgcgcg
      361 tccaaacggc gcgcgacgag ccggcgatac gcgacgatgc aaagtgcgcc gaagacgagc
      421 gcgactatgg cgcagaggac caccgggccg gactctgccg acgcatacgc ttcctgcggg
      481 gtccgcaagt aggtgagagg cgttgcgtcc agccgcacgc cccacgcatc gtaaataccg
      541 aggtcggccg cggtgaacag cgtgaccacg acgaccaagg cataggtata tgtggcaagt
      601 gctccgcgaa ccacactcgg ccgcaccaca cggccaagga tgatgagcag tgatggcact
      661 atcgtcgcat acgcagcagc ggaaaggtcc atgcgcgcgc cgttgaggaa cacccggccg
      721 atggtgccga tgcccaactg tgcggcagcg tgatgttcgt agacgatgaa gatgccccgc
      781 gcgacgagaa acgcggccgt ccagaacagc agatagcaaa cgagaaaccg cacgcgatcg
      841 gcgacgacgg atcgcgcggc cggctgggat tggaggtgca cgccgaacga tagaagagca
      901 tcgcggcggc ggccagtgcg gtcgcgttcg gcgtcctcga cgggggacgc aacctgtgtc
      961 ggaatgcggt cactcactgt cggcaatgca cgcaggacat cggcagacaa caccatcttc
     1021 cgatgaggct gagctgtagt atcgtacgtg cgccatctgc acatcgtctc gcgcgaaccg
     1081 cgaagacggg gcgtgtattg gtacaacgat tgcgcgacgc agtcgctcgt cgagtcgcgc
     1141 gctcaccccc tacgagaggc taacgtgccg agcgtacgca tagcagggag taaggctctc
     1201 atgatcgcgg cgacgctcgc gacgggattc gcctttagcc tgccgtccac tgccgcggcg
     1261 cagtacttcg gccgcaacaa agtccagtac cagacgttcc acttcaagcg gctcaagacg
     1321 ccgcacttcg acgtctacta ctatgccgcg gagtcgctcg cggcagtcga tgcggcgcgc
     1381 atggccgagc ggtggtacgc gcggcacagc aagaccctga ccgacgaatt cgatcggcgg
     1441 tcgatcatcc tctacgccaa ccacgccgat ttcgagcaga ccaacgtcat cggcgggttc
     1501 atcgaccaga gcacaggcgg cgtgaccgag ggattgcgga gccgcgtcgt gctgccgttc
     1561 accggcgtgt acgccgacga cgaccacgtg atcggccacg agctgacgca cgtgttccaa
     1621 tacgacatcg ccggaaagat gaagggcgga ggcatgatcg cgctcgaccg cctgccgctg
     1681 tggctcatcg aaggcatggc cgagtatctc tcgttaggca ggaacgaccc gaacaccgcc
     1741 atgtggctgc gcgacgcggc gctgcgcaac gacattccga cgatcaagaa gctgagcacg
     1801 gacccgcgct atttcccgta ccgttacggc gaggcgctgt gggcctacat tggcgggcgc
     1861 tggggcgacg acgtggtcca gcagctctac cgcgcgtcgc tgcagagcgg attcgagttg
     1921 ggcatccgcc gcaccctcgg catgagcagc gacacgctgt cggcgcagtg gctgcaggcc
     1981 atccgcgacg cgtatctccc gctcatgcag gggcggcagg cgcccgattc gttaggcaag
     2041 cgcctcctcc ggccgggcag caagtacggc gacatggatg tgtcgcccgc cctgagcccc
     2101 aacgggaagt acctcgcgtt cctgacgacg cgcggcctgc tgaacatcga cctgtacgtc
     2161 gccgacgcgc agacgggaca gatcgtgaag aagctcaccg ggccgaacag cgaccagcat
     2221 ttcgacgcca tcagcttcat caactcgtcg ggctcgtggt cgccggacgg caacgacttc
     2281 gcgttcatc
//
LOCUS       DASUCJ010000988         1375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_545037, whole genome shotgun sequence.
ACCESSION   DASUCJ010000988 DASUCJ010000000
VERSION     DASUCJ010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545037"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..573)
                     /locus_tag="VFU90_11725"
     CDS             complement(94..573)
                     /locus_tag="VFU90_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5480499.1"
                     /translation="MTHLRGFHALDRADCAILRILQENARESFAEIGKQVGLSATSVA
                     ERIRRLETAGANERYGAQLNAAKLGYPVTAFVLARPNGPDARFVKVARERPEILECYR
                     VTGEFSFIARAVLKDVAHLEALLDHLEPAAVHIVTLVVLSTSFDGVPLSIESPEGLN"
     gene            660..>1375
                     /locus_tag="VFU90_11730"
     CDS             660..>1375
                     /locus_tag="VFU90_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480500.1"
                     /translation="MARTDCTLVTCDAFPALDPDDRLLAGELRRRGLRVAIAVWSDPA
                     VDWSAGRLTVLRSTWDYHRRHAEFVAWLDRIAPLTTVRNAPPLIKWNAHKFYLRDLEL
                     RGIPVVPTAWLTQGTRCRLTELCASRGWDPVVIKPALGAASHDVVLAANSNGVTLSGA
                     PKARSSVTLSGAPKARSRRAPVSLDEAQAHLDALLAGEDALVQPYLQSVETYGERALM
                     FVRDRYSHAVAKEPFDTVLAV"
BASE COUNT          227 a          488 c          423 g          237 t
ORIGIN      
        1 cacatggact ctgtccgcac tctctccgca gcaaaacctc gagatcccgc aaagcgacgc
       61 cggcgcgatc ctcgaacgca tcgaggtacc aatctaattc aagccttcgg gtgactcgat
      121 gctcagcgga acgccgtcga atgaggtgga caacacgacc agcgtcacga tgtgcaccgc
      181 agcgggttcg aggtggtcga gcagtgcctc gagatgcgcc acgtctttca gcacggcgcg
      241 cgcgatgaac gagaactcgc cggtgacgcg gtagcactcc aaaatttccg ggcgctcgcg
      301 cgcgactttc acgaagcgcg cgtccggccc attcggcctg gcaagtacga acgccgttac
      361 cggatagccg agcttggcgg cgttgagctg cgcgccgtag cgctcgtttg cgccggccgt
      421 ttcgagccgg cgaatgcgct cggcaacgct ggtagccgac aggcccacct gctttccgat
      481 ctccgcaaac gattcgcgcg cgttctcttg caagatgcga aggatcgcgc agtcggcccg
      541 atccaacgcg tggaaaccgc gcaaatgcgt cacggatgcc tcctatttca cggcgtcgcg
      601 gcggatattc gtggtatttt ggctgttcct tcgccgcacg ccggtgttag actcgtaacg
      661 tggcgcgtac cgactgcacc ctcgtaactt gcgacgcgtt cccggcgctc gaccccgacg
      721 accgtctgct ggccggcgag ctccgccgcc gcggcttgcg cgtggcgatc gcggtgtgga
      781 gcgatcccgc cgtcgattgg tccgccgggc gcctcaccgt cctgcgatcc acctgggact
      841 atcatcgccg gcacgccgag ttcgtggcct ggctcgatcg gatcgccccg ctcacgacgg
      901 ttcgcaacgc gccgccgctg atcaagtgga acgcccacaa gttctatctc cgcgatctcg
      961 aactccgcgg catcccggtc gtcccgacgg cttggctgac gcaaggcacg cgttgccgcc
     1021 tcaccgagct gtgcgcatcg cgcggctggg atcccgtcgt catcaagccc gccctcggcg
     1081 ccgcctccca cgacgttgtt ctagcggcca actccaacgg tgtcaccctg agcggagcgc
     1141 cgaaggcgcg cagcagtgtc accctgagcg gagcgccgaa ggcgcgcagt cgaagggcgc
     1201 ccgtctcgct cgacgaggcg caagcgcacc tcgacgcgct gctcgcgggc gaagacgccc
     1261 tcgtccagcc gtacctgcaa tcggtcgaaa cctacggcga gcgcgcgttg atgttcgttc
     1321 gcgaccgcta ctcgcacgcc gtcgccaaag agccgttcga caccgtgctc gccgt
//
LOCUS       DASUCJ010000989         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_239507, whole genome shotgun sequence.
ACCESSION   DASUCJ010000989 DASUCJ010000000
VERSION     DASUCJ010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239507"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>1120
                     /locus_tag="VFU90_11735"
     CDS             61..>1120
                     /locus_tag="VFU90_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU5480501.1"
                     /translation="MPDSGVIVNAQQVTPAGVQSVFDGRVGGVRFGKSDDDIWVAVAG
                     SVYHLAWRDNRVISSASYPGHPGVQAVAVDPATGRAAVSTVGRGATNPEGELPGGIAR
                     IARSDAVQLAFFDAEGGGVSGSAFASGAVGDYAAGAPAIARTRNTTGHRVAVLPLTAN
                     DRLTVIDADRGTVLKSITLGVAPIAAVVSSDGSIAWVTNLGGAKPKRGDRSATECCYE
                     RAERVRIDRRGIAEPGSVVRVDLVTGKVTNSLAVGRHPTALAWDTLHARLYVANGNDE
                     SISVIDTREFRTVGRLEIGSRRYNHTGEAPTALAIAPDGQTLYVAVGALNAVAVFDVS
                     TSHLRGLIPTGWYPSSVDV"
BASE COUNT          171 a          374 c          393 g          182 t
ORIGIN      
        1 attctccttc caatgctgct cgcgcagggt gtgtagcagc cggcctcccc gaggcgcgcc
       61 gtgcccgatt cgggcgtgat cgtgaatgct caacaggtga cgccggccgg cgtccagtcc
      121 gtgttcgacg gacgcgtcgg cggcgtgcgc ttcgggaaat ccgacgacga catctgggtc
      181 gcggtagccg gctccgtgta ccacctcgcg tggcgcgaca accgtgtgat ctcgagcgcg
      241 tcctatcccg gacaccccgg cgtgcaggcg gtcgcggtgg atcccgcgac cggtcgagcc
      301 gcggtgagca ccgtgggacg tggggcgacg aacccggagg gtgagctccc cggcggcatc
      361 gcgcgcatcg cgcgaagcga tgccgtgcaa ctcgcgttct tcgatgcaga gggcggcggc
      421 gtgagcggct cggcgttcgc gtccggggcc gttggcgact atgcggccgg cgcccccgcc
      481 atcgcgcgca cccggaacac caccgggcac cgtgttgcgg tgctccctct caccgcgaac
      541 gaccgcctaa cagtgatcga cgccgatcgg ggcactgtat tgaagtccat cacgctcgga
      601 gtggcaccga tcgcggccgt ggtctcgagc gacgggagca tcgcctgggt gacgaacctc
      661 ggcggcgcca agccaaagcg tggcgatcgc tcggcaacgg aatgctgcta cgagcgcgcg
      721 gagcgggtgc gtatcgatcg acgtggcatc gccgagccgg gcagcgtggt gcgcgtcgat
      781 ctcgtgaccg ggaaggtgac gaattcactg gccgtgggtc ggcacccgac ggcgctcgcg
      841 tgggacacgc tgcacgcccg gctctacgtc gccaacggca atgacgagtc gatctccgtc
      901 atcgacacgc gggagtttcg aaccgtcggc cgactcgaga tcggaagccg gcgttacaat
      961 cacaccggtg aagcgccgac ggcgctcgcc atcgcgccgg acggacagac tctctatgtc
     1021 gccgtagggg ctctgaacgc cgttgccgtt ttcgatgtct cgacttcgca tctgcgtggg
     1081 ctcattccga ccggctggta cccgtcgagc gtcgacgtga
//
LOCUS       DASUCJ010000990         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_423108, whole genome shotgun sequence.
ACCESSION   DASUCJ010000990 DASUCJ010000000
VERSION     DASUCJ010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423108"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFU90_11740"
     CDS             <1..550
                     /locus_tag="VFU90_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEU5480502.1"
                     /translation="LLVGLDGEKKMSKSLGNYVGIDEPASEQFGKLMKIGDDLIATYA
                     RLAAFRSQAECELLAAQLADGRVNPMEEKKRLAEAIVARYHGSEAARGAREHFERTVQ
                     RKELPAGEIPEVDGTGCTRVTELLVRAGFAESRRAAERLISGKGVRVDGIVVSDPRAA
                     WNTMGPAVLSVGSRKFARIVVS"
     gene            563..790
                     /locus_tag="VFU90_11745"
     CDS             563..790
                     /locus_tag="VFU90_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480503.1"
                     /translation="MAAVTYSAGTPIEGRKFVCKRCKREYSYPQPGDRPIRCECGWWY
                     LNDGNRIREVYNQRIEPYRTPRDVRALFQRS"
     gene            complement(803..1684)
                     /gene="hslO"
                     /locus_tag="VFU90_11750"
     CDS             complement(803..1684)
                     /gene="hslO"
                     /locus_tag="VFU90_11750"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU5480504.1"
                     /translation="MPDLLLSATVANDGVAVFSAITTGTVREIRARHDLSPTATAAVG
                     RLLTGAVLLAANLKGSERISLQISGDGPIGGVTADAWLLDHETIGARAYARNPHADLP
                     FDERGKFDVSGSIGDGVLQVTKSYEVGQPYVGVVPLHSGEIAEDIALYFAQSEQIPSV
                     VALGVLANPTGVVAAGGIVAQVLPGTEEGAIVALEQRALAMRPVTTLVADGADARGLL
                     DEIAGHVDLRALRSTHVRFACLCTKRKVEAALLGLGREELERMRIERERTEATCEFCR
                     CVYLFSRQELAALADRI"
     gene            1759..>2026
                     /locus_tag="VFU90_11755"
     CDS             1759..>2026
                     /locus_tag="VFU90_11755"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU5480505.1"
                     /translation="MRVLVLHGPNLNLLGNREPDIYGSETLADVNDVIAAAASDLGAA
                     VSCEQHNGEGELIDALHESRERYDAVIINPGAYAHYSHAIADAIA"
BASE COUNT          402 a          602 c          658 g          364 t
ORIGIN      
        1 gttgctggtg ggactcgacg gcgaaaagaa gatgagcaaa tcgcttggaa actacgtcgg
       61 aatcgacgaa cccgcgagcg agcagttcgg caagctgatg aagatcggcg acgatctgat
      121 cgccacctac gctcggttgg cagcgttccg ctcgcaagcg gaatgcgagc ttctggcagc
      181 gcagctcgcc gacgggcgcg tcaatccgat ggaggagaag aaacgtctcg ccgaagcgat
      241 cgtcgcgaga tatcacggtt ccgaagcggc gcgcggtgcg cgcgaacact tcgaacgaac
      301 ggtccagcgg aaggagctgc cggccggcga gattccggag gtggacggaa cgggctgtac
      361 gcgcgtcacc gaattgctgg tgcgagcggg cttcgcggag agccggcgag cggccgagcg
      421 acttatctcg gggaaaggcg tgcgcgtcga tggtatagtc gtgagcgatc cgcgagcggc
      481 gtggaacacg atgggaccgg ccgttctctc ggtggggtcg cgaaagttcg cacggatagt
      541 ggtttcataa ggaagggcgg taatggcggc agtgacttac tcggcgggga cgccgatcga
      601 aggacgcaag ttcgtttgta aacgctgcaa gcgcgaatac tcctatcctc agcccggcga
      661 ccgcccgatt cgctgcgaat gcggttggtg gtatcttaac gacggcaatc gcattcgcga
      721 agtctacaat caacgaatcg agccgtaccg gacgccgcgc gacgtccgcg cgctctttca
      781 gcggtcgtag ctgagccggg cgtcaaatcc tatcggctag agcggcgagt tcctggcgac
      841 tgaagagata gacgcaacga cagaactcgc acgtcgcctc ggtccgttcg cgttcgattc
      901 tcatgcgctc caactcctcg cggcccaaac ctagtaacgc ggcttcgact ttccgtttgg
      961 tgcagagaca ggcaaagcga acgtgcgtcg aacgcaaggc gcgcaagtcg acatgtcccg
     1021 ctatctcgtc gagcagtcca cgcgcgtcgg ctccgtccgc gacgagggtc gtcaccggtc
     1081 gcattgcgag cgcacgctgc tcgagggcca cgatggcgcc ttcctccgtg cctggaagca
     1141 cttgtgcgac gatcccgccg gcggcgacaa ctccggttgg gtttgcaagt acgcccagcg
     1201 cgacgacgct ggggatttgc tcggactgag caaaatatag ggcaatatcc tcggcgatct
     1261 cgccggaatg gagtggcacg acgccgacgt aaggttgtcc gacctcgtag gacttcgtga
     1321 cctggagcac gccgtcgccg atggaaccgg agacgtcgaa ctttccgcgt tcgtcaaagg
     1381 gaagatcggc gtggggatta cgagcgtagg cgcgcgcacc gatggtttcg tggtcgagga
     1441 gccatgcgtc ggcggtcacg ccgccgatcg gcccgtcccc cgatatttgc agcgagatgc
     1501 gttcgcttcc cttaaggttt gctgccagca gcaccgcccc ggtgaggagc ctcccgaccg
     1561 ccgcagttgc ggtgggtgac aaatcgtggc gcgcccgaat ctcacgtacg gttcccgtcg
     1621 ttatggccga aaatacggcg acaccgtcat tcgcaacggt ggcgctgagg agtaggtcgg
     1681 gcatcaatgc gtctctctcg aaaccctcag ccgtttcctt caacaggttt ttcggatgcg
     1741 tcctgcggaa aggaccgtat gcgcgttctc gtcttgcacg gcccaaactt gaacttgctt
     1801 ggcaaccgcg aacccgacat ttacggctcc gagacgcttg cggacgtcaa cgacgtaatc
     1861 gccgccgccg cgagcgacct cggagcagcc gttagctgcg aacagcataa cggagagggc
     1921 gaactgatcg atgcgctcca cgagtcgcgc gaacggtacg atgcagttat tatcaacccc
     1981 ggcgcgtacg cccattactc gcacgcgatc gcggatgcga tcgcag
//
LOCUS       DASUCJ010000991         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_713658, whole genome shotgun sequence.
ACCESSION   DASUCJ010000991 DASUCJ010000000
VERSION     DASUCJ010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713658"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFU90_11760"
     CDS             complement(<1..336)
                     /locus_tag="VFU90_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU5480506.1"
                     /translation="MVGAISMLMLAACATTPAYPPAPQHLAGVDYNYHIGPSDVVNVI
                     VWRNPELSMSVPVRPDGKITTPLVDDLPALGKTPTELERDLEKALAKYIRDPVVTVIV
                     TTPVGPVQEQ"
     gene            682..>1054
                     /locus_tag="VFU90_11765"
     CDS             682..>1054
                     /locus_tag="VFU90_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480507.1"
                     /translation="MMKNFRVARGVAWQILLAVSIAVAPPLAVAATDPTNTTLAAADA
                     PASKDALFGNDLAIAPKSPWRGYVRGEIARTYASPSHWSKMLVSSEVDAEGSISANVK
                     YHLGARFDYDFVYDANDFYPPE"
BASE COUNT          198 a          332 c          343 g          181 t
ORIGIN      
        1 ctgctcctgc accgggccga ccggcgtggt cacgatcacc gtcaccaccg gatcacggat
       61 gtacttcgcg agcgccttct cgaggtcgcg ttcgagctcg gtcggcgttt tgccgagggc
      121 agggagatcg tcgacgagcg gcgtcgtgat cttgccgtcc ggacgcacgg gaaccgacat
      181 cgacagctcc gggttgcgcc agacgatcac gttgacgacg tccgagggcc cgatgtggta
      241 gttgtagtcg acacccgcga ggtgctgggg cgcgggcgga tacgccggcg tcgtcgcgca
      301 agccgcgagc atcaacatcg aaattgcgcc aaccatcagt gcacgccaca ggcgcccggt
      361 cgggtcgctg gagcaggcgg attgttgtat tagccggttc atgcgtcctc cgagggcggg
      421 atgcggtaaa cggaacgaag gatcatagca ggagcaacgt atcgtaccga gcgaaaatcc
      481 caagcacgct ccgtgccatg cgattctagc gaaggcgatc gacaagcgct tgcgcgaccc
      541 gatgaacgtc gaagcgaccg ggcctgatcg atcggcgaac gggcgggtta gacggccgat
      601 acgacacgcg gttgcgcggt tgcctgtaat ctgcccattt ttactggctt tcggctgccg
      661 cccaccgcag aatcgggacc catgatgaag aattttcgcg tcgcgcgcgg agttgcatgg
      721 caaatcctgc tcgctgtctc gatcgcggtc gcaccgccgc ttgcggtggc cgcgacggac
      781 ccgaccaaca cgacgctcgc agccgcggat gcaccggcgt cgaaggatgc gctgttcggc
      841 aacgatctcg cgatcgcacc gaagtcgccg tggcgcggct acgtgagagg tgagatcgcg
      901 cgcacgtacg cgtcaccgtc gcactggtcg aaaatgctgg tgagctccga agtcgacgcc
      961 gagggctcga tctccgccaa cgtcaagtac cacctcggcg cgcgcttcga ttacgacttc
     1021 gtctacgacg cgaacgactt ctacccaccc gaag
//
LOCUS       DASUCJ010000992         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_362231, whole genome shotgun sequence.
ACCESSION   DASUCJ010000992 DASUCJ010000000
VERSION     DASUCJ010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362231"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFU90_11770"
     CDS             complement(<1..315)
                     /locus_tag="VFU90_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU5480508.1"
                     /translation="MAVHPTAIVEPGVSIGRNTAIWDNVHVRHSTRIGADCIIGEKSY
                     IAYGVRIDDLVKINAFVYVCTGVTIERGVMISAGVTFTNDRFPRATTIGLDRLLPSDP
                     DEK"
     gene            complement(317..1357)
                     /locus_tag="VFU90_11775"
     CDS             complement(317..1357)
                     /locus_tag="VFU90_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5480509.1"
                     /translation="MKPLRYAIVGAGAIARAYESAFDGVKGAEIVAVCDVRRAAAEEF
                     AARVGCKAYGSLRAMLGGAEFDAAIVCTPPISHEPVTCELLERGKHVLCEKPLAVNVG
                     AAQRMLSAAARSGKLLTMASKFRYAADVRKARELVMAGAIGELVFMENAFTSHVDMRH
                     RWNSDASVSGGGVLIDNGTHAVDILRFFLGNLCDVQVVEGLRIQGLAVEDTVRLFVHN
                     DAGMMGTADLSWSIDKALETFLRIYGSAGTILVGWKESRFRRHGESDWHTFGGGYDKI
                     QAFRDQLENFCAAIRGTAALVVTPHDALASVHVIQAAYTALERARWERISSQIDEFAI
                     APLAIGRGAAAS"
     gene            complement(1354..>2212)
                     /locus_tag="VFU90_11780"
     CDS             complement(1354..>2212)
                     /locus_tag="VFU90_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5480510.1"
                     /translation="ERITDRTKAIVVTHLFGNPCDIEEIVALGRRAGIPVIEDCAQAF
                     LAGYNGRFVGTFGSVGCFSLQQGKHITTGEGGIVVSDDDALARRMYLFINKAWGYGDP
                     NPDHYFLALNYRMSELTGAVACAQLRKLPTVVARRRMLAELLTERLRGVPGILTPAIH
                     RGAQHSYWKYCIRTTDTMPKDAVVEIARLLKEDGIATVPRYIQKPAFMCEVFQQRKTF
                     GDSGYPFSIASPDALDYSPDGYPGALAALERILVIPWNDRYDEDDVAYIADAVAAAAA
                     TVSEARVAS"
BASE COUNT          369 a          737 c          722 g          384 t
ORIGIN      
        1 cttttcgtcg ggatcggacg gcagcaaccg atcgagcccg atcgtcgtcg cgcgcggaaa
       61 ccggtcgttg gtgaacgtca cgccggcgga gatcatgacg ccgcgctcga tcgtgacccc
      121 cgtgcaaacg tagacgaagg cgttgatctt gacgaggtcg tcgatccgga cgccataggc
      181 gatgtaactc ttctcaccga tgatgcagtc ggcgccgatc cgggtggaat ggcgaacgtg
      241 cacgttgtcc catatcgccg tgttccggcc gatcgagact cccggctcga cgatcgcggt
      301 gggatggacg gccatgctag gaggccgccg ccccgcggcc gatcgccagc ggcgcgatcg
      361 cgaactcgtc gatctggctg ctgatgcgct cccagcgcgc gcgctcgagc gcggtgtagg
      421 ctgcctgaat cacgtgaacc gaagcgagcg cgtcgtgcgg cgttaccacc agggctgccg
      481 tcccgcgaat cgccgcgcaa aagttctcga gttgatcgcg gaaggcctgg atcttgtcgt
      541 agccgccccc gaacgtgtgc caatcacttt cgccgtgtcg gcggaaacgc gattccttcc
      601 agccgaccag aatcgttccg gcgcttccgt agatgcgcag gaacgtttcg agcgctttgt
      661 cgatgctcca cgaaagatcg gcagtcccca tcatgccggc gtcgttatgc acgaacagcc
      721 ggaccgtgtc ttcgacggcc aggccttgaa tgcgcaaacc ttcgacgact tgcacgtcgc
      781 acagattccc caggaagaac cgaaggatgt ccaccgcgtg cgttccgttg tcgatgagca
      841 cgccgccccc gcttacggaa gcgtcggaat tccagcgatg ccgcatgtcg acgtgcgacg
      901 tgaaggcgtt ctccatgaac accagctcgc cgatcgcgcc cgccatcacc agctcgcgcg
      961 cctttcgtac gtcggccgcg taacggaact tcgaagccat cgtcagcagc ttgccgctgc
     1021 gggcggcggc cgacagcatt cgctgggcgg cgccgacgtt gaccgccagc ggcttctcgc
     1081 aaagcacgtg cttgccgcgc tccagcaatt cgcaggtgac cggttcgtgc gaaatcggcg
     1141 gcgtgcacac gatggcggcg tcgaattcgg caccgccgag catggcccgc agcgaaccgt
     1201 aggccttgca tccgacgcga gcggcgaatt cttcggcggc cgctcgccgc acgtcgcaga
     1261 ccgcgacgat ttcggctccc ttgacgccgt cgaacgcgct ctcgtacgcg cgcgcgatcg
     1321 cgccggcgcc gacgatcgcg taacgcagcg gcttcacgac gccacccgcg cttccgatac
     1381 ggtcgcggcc gcagcggcga cggcatcggc gatgtacgcc acgtcgtcct cgtcgtagcg
     1441 gtcgttccac gggattacca aaatgcgttc gagcgctgcc aacgcgccgg ggtagccgtc
     1501 cggcgagtag tcgagcgcgt ccgggctcgc gatgctgaac ggatagccgc tgtcgccgaa
     1561 cgtcttgcgc tgctggaaga cctcgcacat gaaggcgggc ttctgaatgt agcgcggaac
     1621 ggtggcgatc ccgtcctcct tcaggagccg ggcgatctcg acgaccgcgt ccttgggcat
     1681 cgtgtcggtg gtgcggatgc agtatttcca gtagctgtgc tgcgcaccgc ggtgaatcgc
     1741 cggggtgagg atgccgggca ccccgcgcag ccgttccgtc agcagctcgg ccagcatgcg
     1801 ccggcgcgcg acgacggtgg gcagcttgcg cagctgcgcg cacgccaccg caccggtcag
     1861 ctcggacatg cggtagttga gcgccaggaa atagtggtcg ggattcggat cgccgtaacc
     1921 ccacgcctta ttgatgaaca gatacatgcg ccgcgcgagc gcgtcgtcgt cgcttacgac
     1981 gatgccgccc tcgcccgtcg taatgtgctt gccctgctgc aggctgaagc agccgacgct
     2041 tccgaacgtg cccacgaagc ggccgttgta gccggccaga aaggcttgcg cgcagtcttc
     2101 gatgacgggg atgccggcgc gccgccccag cgcgacgatc tcctcgatgt cgcacgggtt
     2161 gccgaagagg tgcgtcacga cgatcgcctt ggtgcgatcg gtgatgcgct cg
//
LOCUS       DASUCJ010000993         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_667667, whole genome shotgun sequence.
ACCESSION   DASUCJ010000993 DASUCJ010000000
VERSION     DASUCJ010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667667"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFU90_11785"
     CDS             <1..1519
                     /locus_tag="VFU90_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5480511.1"
                     /translation="LVIATIALSERPSKPNDRALALLTITVMLGMIMAIIDSTIVNVA
                     LNTIGGNLGATVDEVAWVATGYILANVVVMPLNGWLTALLGRKTFYAISLAVFTVASL
                     LCGTAHSIWLLVFYRVIQGLGGGALQPTAQAILFESYPPERRGAAMAIFGMGAMVGPA
                     IGPTLGGYIVDNASWPLIFYINIPIGIVAFLMTLAFIPNPKFLAKPKGGIDWTALGLL
                     TAGLASLQYVLERGERDNWFASPTIQLLTALAVVSIAAFIYKSLRDRHPIVDLKVFKH
                     RSFSIGSILGIIMGFGLFGTALILPLFFQSILNFTAFDTGMALLPGALSTAVSMLIVG
                     RIFNRIDGRWSIVFGMLIFAWSTWLLGGLSVQAGYWDVFWPRLIQGFGLGFLFVPLTT
                     ISLSDVPIHELAGATGVYTLLRQLGGSFGIAILTTMLTHETAVAWNVLASGVTSTHGY
                     PIGTLTQLVAQQSAMIAYDYLFRVCAIVFVLTTPLVFLIQPKKGRAPAPAAAAAE"
     gene            complement(1530..2429)
                     /locus_tag="VFU90_11790"
     CDS             complement(1530..2429)
                     /locus_tag="VFU90_11790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5480512.1"
                     /translation="MTDASDAVAAHLILGARDEPFLAAMLASITGAASLLIVNDNAPD
                     PSPHANILRDSSFGREGRIVVDRSPFVDFAAARNVCLTLHAQHTTAAWVAFVDADEVH
                     GPEIATIAANLRHVRREHDFVDGYTWHFFQSFDYYTSIERRMAFFRFVPGTRWEGSVH
                     ERLHGIAGGRIALPYVYAHYGHTLEPRRHAEKGRQYSSLGAPGGVLREEQLGDFDVAD
                     YFAPEYPRLLRFRGSHPPAAWPTLERLRPALAETHALTERIVRCQPPAIKLRNAVRKV
                     NYELRWRGRGLDPLARRLMTRTA"
     gene            2489..>3431
                     /locus_tag="VFU90_11795"
     CDS             2489..>3431
                     /locus_tag="VFU90_11795"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5480513.1"
                     /translation="MPSPLRLSVDARVVAEDTRGIGRYARAVLRRLIGRDDVALTLLA
                     FGPLPIRHRAGYALALGSRRFAVRSAVGSDADVVWHPANGTFFASRAPSVATIHDAVP
                     FRYPSADPKRRRHDQRPFLESVRTAARIVAVSAFGRDEVHRVFGVPLERIAVIHHGVE
                     PSFSPGAALPLPPGLVAQRYVLFVGDPIGEPRKNFSLLYDAYRRAWADPGTAPLLVVA
                     GARAPELAGVFHAGNLGDDLTGRTNEPLRALYRGALAVAVASYHETFGMPVLEAMACG
                     TPVIAAESSSLPEIGGSAAYYVAPDDAGAWANALRRVT"
BASE COUNT          536 a         1113 c         1097 g          685 t
ORIGIN      
        1 ccttgtcatc gctaccatcg cgctaagcga acgcccctcg aagccgaacg accgtgccct
       61 ggccctcctc acgattaccg tcatgctggg gatgatcatg gcgatcatcg actcgacgat
      121 cgtcaacgtg gcgctcaaca cgatcggcgg aaacctggga gcgaccgtcg acgaggttgc
      181 gtgggttgca accggataca tcctggccaa cgtcgtcgtc atgccgctga acgggtggct
      241 gacggcgctg ctcggaagga aaaccttcta cgcgatttcc ctggccgttt ttaccgtggc
      301 gtcgctgctc tgcggcacgg cgcattcgat ttggctgctg gttttttatc gcgtcattca
      361 agggctcggc ggcggcgcgc tccagccgac cgcccaggcg attctcttcg aaagctatcc
      421 tcccgagcgc cggggagcgg ccatggctat cttcgggatg ggcgcgatgg tggggccggc
      481 gatcgggccg actctcggcg gctacatcgt cgacaacgcc agctggccgc tgatcttcta
      541 catcaacatc ccgatcggga tcgtagcctt cctcatgacg ctcgccttca ttccgaaccc
      601 gaaattcctc gccaaaccga agggcggtat cgactggacg gcgctcggct tgttgaccgc
      661 cggacttgcg tcgctgcagt acgtgctcga gcgcggcgag cgcgacaact ggtttgcgtc
      721 gccgacgatc cagctactca cggcgctggc cgtcgtctcg atagcggcct tcatttataa
      781 gtcgctccgg gaccgtcacc cgatcgtgga tctaaaggta ttcaaacacc gatccttttc
      841 gatcggatcg atcttgggga tcatcatggg gttcggcttg ttcggtaccg cgttgattct
      901 gccgttgttt tttcagtcga tcctcaattt cacggcgttc gataccggga tggcgttgtt
      961 gccgggcgcc ctttccaccg ccgtatccat gctgatcgtg ggacggatct tcaaccgaat
     1021 cgacggacgc tggtcgatcg tcttcggcat gctgatcttc gcgtggtcta cctggctgtt
     1081 gggcggtttg tcggtccagg cgggttattg ggatgtcttc tggccgcgct tgatccaggg
     1141 cttcggcctc ggatttttgt tcgtgccgct cacgacgatt tcgctctcgg acgtgccgat
     1201 tcacgaactg gcgggcgcga ccggcgtgta tacgctctta cgccagttag gcggcagctt
     1261 cggcatcgct atccttacga cgatgctcac tcacgaaacg gccgtcgcgt ggaacgtgct
     1321 cgcgtcgggt gttacgagca cccacggata tccgatcgga acgctcaccc aactggtcgc
     1381 acagcaatcc gcgatgatcg cgtacgacta tcttttccgc gtctgcgcga tcgtattcgt
     1441 gctgaccacc ccgctggtat tcttgatcca gccgaagaag ggccgcgcgc cggcgcccgc
     1501 ggcggccgcc gccgagtagc ggcacttctt caagccgtac gcgtcatcaa ccgccgcgcg
     1561 agcgggtcga gaccgcgacc gcgccatcgc agctcgtagt ttacctttcg cacggcgttt
     1621 cgcagtttta tcgccggcgg ttggcaccgg acgatacgtt cggtcagagc gtgcgtttcc
     1681 gccaacgcgg ggcgcaagcg ctccagcgtc ggccacgccg ccggcgggtg cgaaccgcga
     1741 aaccgcaaca gccggggata ctccggtgcg aaatagtccg ctacgtcgaa gtcgccgagc
     1801 tgttcttcgc ggagcacgcc tcccggcgca cccagcgacg aatactgacg tcctttttcg
     1861 gcatgtctcc gcggctcgag cgtgtggccg tagtgggcgt acacgtaagg aagcgcgata
     1921 cggccgccgg cgattccgtg caaacgctcg tggacgctgc cttcccagcg cgtgcccggc
     1981 acgaatcgaa aaaacgccat gcggcgctcg atcgaggtat agtaatcgaa cgattgaaag
     2041 aagtgccacg tgtagccatc gacgaaatcg tgctcgcgcc ggacgtgccg gaggttggcc
     2101 gctatcgtcg cgatctcggg accgtgtacc tcgtcggcat cgacgaacgc cacccacgcg
     2161 gccgtcgtgt gttgcgcatg cagggtcaaa cagacgttgc gcgctgccgc aaaatctaca
     2221 aacgggctac gatcgacgac gatccttccc tcgcgcccga acgagctgtc ccgcagtatg
     2281 ttcgcgtgcg gcgacgggtc gggtgcgtta tcgttgacta tcaacaggct cgccgctccg
     2341 gtgatcgacg cgagcatcgc agctagaaac ggctcgtcgc gagccccgag gatcaagtgc
     2401 gcggcaacgg cgtcgctcgc atcggtcatc gaggcggccg ttcgcgcggc ggggaggagt
     2461 ttccgccggg cgcgaggagt ctggcgcgat gccgtcgcct ctgcgccttt cggtcgacgc
     2521 gcgcgtcgtc gccgaggata cgcgcgggat cggacgctac gctcgcgcgg tcttgcgacg
     2581 gctgatcgga cgcgacgacg tcgcgttgac gctgctcgcg ttcgggccgc tgccgattcg
     2641 gcatcgagcc gggtatgcgc tcgcgctcgg ttcgcggagg ttcgcggtgc gctcggccgt
     2701 cggcagcgac gccgacgtcg tgtggcatcc ggcgaacggc accttcttcg cctcgcgcgc
     2761 cccaagcgtc gccaccatcc acgatgcggt gccgtttcgc tacccgagtg ccgatcccaa
     2821 gcggcggcgc cacgatcaac ggccttttct cgaatcggtt cgaacggcgg cgcggatcgt
     2881 cgcggtctcc gcgttcgggc gcgatgaagt gcatcgcgtg ttcggcgtac cgctcgagcg
     2941 cattgcggtg atccatcacg gggtcgagcc gtcgttctcg ccgggggccg ccctgccgct
     3001 tccgccggga ttggttgcgc agcggtacgt gctgttcgtg ggcgatccga tcggcgagcc
     3061 gcgtaagaac tttagccttt tatacgacgc ctaccgtcgc gcttgggccg atccgggcac
     3121 ggcaccattg ctcgtcgttg ctggggcgcg cgcgccggag ctcgcgggcg ttttccacgc
     3181 cggaaacctc ggcgacgatc tcaccggaag gaccaacgaa ccgctgcgcg cgctctaccg
     3241 cggagccctc gccgttgccg tagcgtcgta tcacgaaacc ttcggcatgc ccgtactgga
     3301 ggcgatggct tgcggaacgc ccgtaatcgc cgcggagtcc agttcgctgc cggagatcgg
     3361 gggaagcgcc gcttattacg tggcacccga cgacgccggt gcctgggcga acgcgttgcg
     3421 gcgcgttacc g
//
LOCUS       DASUCJ010000994         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_10630, whole genome shotgun sequence.
ACCESSION   DASUCJ010000994 DASUCJ010000000
VERSION     DASUCJ010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10630"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1642
                     /locus_tag="VFU90_11800"
     CDS             425..1642
                     /locus_tag="VFU90_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5480514.1"
                     /translation="MSRTLLPAAVLVFAALAGACTVAIPVASAQTIRLGEINEYKQFP
                     AFLAPYRKGMQLALDEINAKGGVLGRRLEIVSRDDNGVPGDAVRAAEELTTREHVALL
                     MGTFASNIGLAVSDFARQRKVLFIAAEPLTDKLVWQDGNAYTWRLRASTYMQTAMLVP
                     AALATGKKRWAIVYPNYEYGQAATETFKQQMIARSGSGLEFVEQPVPLGKIDAGAVVQ
                     ALVDDHPDAIFSSLFGPDLARFVREGELRGLFKDRPVFDLLGGEPEYLDPLKDEAPAG
                     WYVTGYPWYAIDTPAHRAFLDAYRAKFDDYPRLGSVVGYSTVKTAAAAIARAHSTDTA
                     KLVAAMANLHVATPFGDVTFRAIDHQSTMGAFVGRLAVRDGKGVMVDFHYADGKDYLP
                     PDAVVRRLRPAER"
     gene            1653..>1871
                     /locus_tag="VFU90_11805"
     CDS             1653..>1871
                     /locus_tag="VFU90_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480515.1"
                     /translation="MSPASLAVQLLNGLASAASLFLVAAGLTLIFGVTRIVNFAHGSF
                     FMLGAYVAWSTGHALATSLPALPAAFWLA"
BASE COUNT          274 a          645 c          615 g          337 t
ORIGIN      
        1 tgtaaggctc gttgcggatg ggcaccggtc gcggtgcgca gcccgagctc gaagagatcg
       61 acgatccagt tgaaggtgtt gtgtccgttg tccatgacgt gttagcgagt tgaggggtgg
      121 tcgcatcgcc gcacgccggc agcgtgccgg cgaccatcct gcgtcggtcg cgccggcgcg
      181 cggcgtgcgt tccgggtgtc ttccgctgca tccaatcgag caaccggcgt gccaagcgag
      241 cggcggatcg ccgcgcgccg acgatcgtca cctctcagcg acgctgcatc gtgccgggct
      301 gtggccacgg gctgtcgcac acagatttgc taccatcgcg cgcacgacga gccacgaccc
      361 tcgaccggcc cgcttttgct cgagtcgtcc ggcttgcccg cccccacgcc tcgctggaga
      421 cagcatgtcc cgaacccttc tccccgccgc ggtgctcgtg ttcgcagcgc ttgccggcgc
      481 ctgcaccgtt gccattccgg tcgcctccgc gcaaacgatc cgactcggcg agatcaacga
      541 gtacaagcag tttcccgcgt ttctcgcgcc gtaccgcaag gggatgcagc tcgccctcga
      601 cgagatcaac gcgaagggcg gcgtactcgg acggcggctc gagatcgtct cgcgcgacga
      661 caacggcgtg cccggcgacg cggtgcgcgc cgccgaggag ctcacgacgc gcgagcacgt
      721 cgcattgctg atggggacct tcgcgtcgaa catcgggctc gccgtatcgg atttcgcacg
      781 ccagcggaag gtgctgttca tcgctgccga gccgctcacc gacaagctcg tctggcagga
      841 cggcaacgcg tacacgtggc gcctgcgcgc gtcgacgtac atgcagacgg cgatgctcgt
      901 tcccgcggcg cttgcgaccg ggaagaagcg gtgggcgatc gtctacccga actacgaata
      961 tgggcaggct gccaccgaaa cgttcaagca gcagatgatt gcgcgctcgg gcagcgggct
     1021 cgagttcgtc gagcagccgg tgccgcttgg caagatcgac gccggtgccg tcgtacaggc
     1081 actcgtcgac gatcatcccg atgcgatctt ctcgtcgctg ttcggccccg acctcgcccg
     1141 cttcgtccgt gagggggaat tgcgcggact cttcaaggac cgaccggtgt tcgacctcct
     1201 cggcggtgag cccgagtacc tggatccgct gaaggacgag gctcccgccg gctggtacgt
     1261 caccggttat ccgtggtatg cgatcgacac gccggcgcac cgcgcattcc tcgacgccta
     1321 ccgtgcgaag ttcgacgact acccccgcct cggttcggtc gtcggctaca gcaccgtgaa
     1381 gactgccgcc gcggcgatcg cgcgcgcgca ttcgaccgat acggcaaagc tcgtcgcagc
     1441 gatggcgaac ctccacgtgg cgacgccgtt cggcgacgtg acattccgtg cgatcgacca
     1501 ccagtcgacg atgggcgcgt tcgtcgggcg ccttgcggtt cgtgacggca agggcgtgat
     1561 ggtcgatttc cattacgccg acggcaagga ttacctgccg cccgacgccg tcgtgcggcg
     1621 cctgcgtccg gccgagcgct gatcgcgttg aggtgtcgcc cgcatcgctc gcggtccagc
     1681 tgctgaacgg cctcgcgtcg gcggcctcgc tgttcctcgt cgcggcgggt ctcacgctga
     1741 tcttcggcgt gacgcggatc gtcaacttcg cgcatggctc gttcttcatg ctcggagcct
     1801 acgtggcctg gtccacaggt cacgcgctcg cgacatccct tcccgctttg ccggcggcgt
     1861 tctggcttgc g
//
LOCUS       DASUCJ010000995         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1263854, whole genome shotgun sequence.
ACCESSION   DASUCJ010000995 DASUCJ010000000
VERSION     DASUCJ010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263854"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..>1082
                     /locus_tag="VFU90_11810"
     CDS             180..>1082
                     /locus_tag="VFU90_11810"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480516.1"
                     /translation="MADIAADIRRVLDDQIPDLRRDARIMELLGASIEGNVETLLHAL
                     RYDIAVERIEAPTAALEYARRLAQHGVPVNALVRAYRLGQRRMNELVFAELHLIDMPD
                     ATRVAVIEAMSAAMFGYIDWMSQQVVAVYEDERERWLENQNSLRGLRVRELLVATKKV
                     DVDAATEAIRYPLRWHHVGLVLWYSDRGTDGDELSRLQRFLREMAEAVDAGASPLFVA
                     ADQGCGWAWLPFRAAAADVVAKVRRFAQAYPDPPSVAIGSMSAGVEGFRRSHREADEA
                     RAVAIVSARDGATVIAASDPGLSVV"
BASE COUNT          183 a          334 c          387 g          178 t
ORIGIN      
        1 caaagctagg agcggagtac ggcccggcac agtagcacgc cgacaaaacc gcgcgacgcc
       61 gttatcgccg gaagacaacc gctggggcta acgtcgtcag cgtggagaaa ccgagcctcg
      121 acagcgacgt cggggcgagc cgttatgtcg ccgaggtcgc cggccgattt cacgatcggt
      181 tggccgacat cgctgccgac attcgccggg tcctcgacga ccagatcccc gacttgcgcc
      241 gcgacgcacg gatcatggaa ctcctcgggg cgagcatcga gggcaacgtg gaaacgctgt
      301 tgcatgcatt gcgctacgac atcgcggtcg agcgcatcga ggcgcccacg gcagcgctcg
      361 agtatgcgcg ccgattggca cagcacgggg tgcccgtcaa cgcattggtg cgcgcctacc
      421 ggctcggtca gcgtcggatg aatgaacttg tcttcgccga gctgcacctg atagatatgc
      481 cggatgcgac acgggtggcc gtgatcgagg cgatgagcgc cgcgatgttc ggttacatcg
      541 actggatgtc gcagcaggtc gtcgccgtgt acgaagacga acgcgaacgg tggctggaga
      601 accagaacag cctgcgtggc ctgcgggtgc gtgaacttct ggtcgccacc aagaaggtcg
      661 acgtcgatgc ggcgacggag gcgatccgtt acccgctgcg gtggcaccac gtggggctgg
      721 tgctgtggta ttcggatcgc ggcacggacg gcgatgagct ttcccggctg caacggtttc
      781 ttcgcgaaat ggccgaagcc gtcgatgccg gcgcaagtcc gcttttcgtt gccgccgatc
      841 aaggctgcgg ctgggcgtgg ttgccgttcc gcgccgccgc cgccgacgtc gtggccaaag
      901 tgcgccggtt cgcgcaggcg tacccggacc cgccgagcgt ggcaatcggg agcatgtccg
      961 cgggcgtcga ggggttccgg cggtcgcacc gggaggcgga tgaggcccgt gccgttgcca
     1021 tcgtgagcgc ccgagacgga gcgacggtga tcgccgcctc cgatcccggg ttgtcggtcg
     1081 tg
//
LOCUS       DASUCJ010000996         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1156437, whole genome shotgun sequence.
ACCESSION   DASUCJ010000996 DASUCJ010000000
VERSION     DASUCJ010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156437"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFU90_11815"
     CDS             complement(<1..555)
                     /locus_tag="VFU90_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU5480517.1"
                     /translation="MNAAIVRAPAANLGDGITTAAQGRPSYETALLQHAAYCEALRRA
                     GVAVTELAPEPRFPDAHFVEDTAVLLETLAILTRPGAGARRGEIETMRAPLARRFGEV
                     AEIVAPGTLDGGDVCDAGDRFFIGISQRTNAAGAEQLAELLKRAGKRCAIVDVRAVPG
                     ALHLKSAMAYAGDGRFVATAALAPH"
     gene            complement(576..>1624)
                     /locus_tag="VFU90_11820"
     CDS             complement(576..>1624)
                     /locus_tag="VFU90_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5480518.1"
                     /translation="GSLLATAVAGALTDRFGDKAVVLWSGVFMGIALVCAAAVRDFHW
                     VIVWLMLYGIGFAAVTPAGSHAIVFFFKKEDRGFAMGVRQCGVPAAGVVGSVLLPSVA
                     LHFGYQWALVAAGAITIVTCSLASRLYREPKELSGERVSLRSMLVEMLQMSRNPQLIL
                     ITLTSMTLVSAQMVLLAFLTLTLVHEAGFVLPLAVGVFTLSQFAAVAGRLAWGWSSDR
                     IFRGSRALPLAVAGVISAAALYAAALLTPQTPAWAIYVLAAAMGFSAEGWFGVGVIAV
                     AEIGGEEHSGSALGVALTWIFFAAFVSPTIFGALAEGLGYRFAWTAFAALTLAGVVPA
                     LLASTFVRRLTAQA"
BASE COUNT          305 a          564 c          530 g          225 t
ORIGIN      
        1 atgcggcgcg agcgcggcgg tagcgacgaa acggccgtct ccggcgtacg ccatcgcgct
       61 cttgagatgc aaggcgccgg gaaccgcgcg gacgtcgacg atcgcgcagc gtttcccggc
      121 ccgtttcagc agttcggcga gctgctctgc gccggccgca ttggtccgtt gcgatatccc
      181 gatgaagaag cgatcgcccg cgtcgcaaac gtcgccgccg tcgagcgtac cgggtgcgac
      241 gatctccgcg acctcgccga aacggcgcgc caacggcgcg cgcatcgttt cgatctctcc
      301 ccggcgcgcg ccggcgcccg gccgggtaag gatcgccagc gtttcgagca agacggccgt
      361 atcttctacg aaatgcgcgt cggggaagcg cggttccggt gcgagttccg tcacggccac
      421 gcccgcgcgg cgcagcgctt cgcaataggc ggcgtgctgc agcagcgcgg tttcgtacga
      481 aggtctccct tgcgcggcgg tcgtgatacc gtcgccgaga ttcgcggccg gggcgcgtac
      541 gatggcggcg ttcatccttt gcgccggttt ctgctttacg cttgcgcggt gaggcgccga
      601 acgaacgtac tcgccagcag cgccgggacg actcccgcca gcgtcagcgc cgcaaacgcc
      661 gtccacgcaa agcgatatcc gagcccttcg gcgagcgcgc cgaagatcgt cggcgagacg
      721 aaggctgcga agaatatcca cgtcagcgcg acgccgagcg cgcttcccga atgttcttca
      781 ccgccgattt cggcgaccgc gatgacgccg acgccgaacc agccttcggc cgagaagccc
      841 atcgcggccg caagaacgta gatcgcccag gccggtgttt gcggagtgag cagcgccgcc
      901 gcgtacagcg cggccgcgga gatcaccccc gcaacggcca gcggcaacgc tcggctgccg
      961 cgaaagattc gatcgctcga ccagccccac gccagccgtc cggcgacggc ggcaaactgc
     1021 gacagcgtaa agacgccgac ggccagcgga agaacgaacc ccgcctcgtg gacgagcgtc
     1081 aacgtcagaa aagcgagcag taccatttgc gcgctgacca gcgtcatcga cgtcagcgtt
     1141 atgagaatca gctgtggatt acgcgacatc tggagcattt cgaccagcat ggagcgcagc
     1201 gaaacgcgtt cgccggacag ctctttgggt tcgcggtaga gccgggacgc gagcgagcac
     1261 gttacgatcg tgatcgcgcc ggcggcgacg agcgcccact gatagccgaa gtgcagggcg
     1321 accgacggca gcagcaccga gcccacgacc cctgccgccg gaacgccgca ctgccggacg
     1381 cccatcgcaa aaccgcggtc ttctttcttg aaaaaaaaga cgatggcgtg gctgccggcc
     1441 ggcgttaccg cggcgaagcc gatgccgtac agcatcagcc aaacgatcac ccagtgaaaa
     1501 tcgcgcaccg cggcggcgca cacgagcgcg atgcccatga acacgccgct ccacagcacg
     1561 accgctttgt cgccgaagcg atcggtcagc gctccggcca ccgccgtcgc gagcagcgag
     1621 ccga
//
LOCUS       DASUCJ010000997         1313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_209545, whole genome shotgun sequence.
ACCESSION   DASUCJ010000997 DASUCJ010000000
VERSION     DASUCJ010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209545"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..562
                     /locus_tag="VFU90_11825"
     CDS             188..562
                     /locus_tag="VFU90_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480519.1"
                     /translation="MTFGAKVLAVAILVGFFVAGSSAAPSRYVYRDVALIAVTGHGTV
                     TSKPHGIDCPGRCRTLFVRGTHLSLRAVAAPGWRFTGFRSTFCNGAHATCVFDMVSSH
                     DCVGGACPTGVFGVRARFVRDS"
     gene            784..>1313
                     /locus_tag="VFU90_11830"
     CDS             784..>1313
                     /locus_tag="VFU90_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5480520.1"
                     /translation="MSTNGNSTGKLLEIKGVVVDAVFPDKLPAIYSALRIPRADGPDL
                     IAEVQQHLGDDRVRAVAMDATDGIARGTDVFDTGAPISVPVGEVTLGRVWNVLGDPVD
                     EKEAAGKDAERWSIHRDPPAFADLSPKVEIFETGIKVIDLIAPFVRGGKIGLFGGAGV
                     GKTVLIQELIHNVAQE"
BASE COUNT          210 a          450 c          447 g          206 t
ORIGIN      
        1 cgccgccccc ttggcagggc ccttgttcgg gatcagcacg gcgacgagca gcccggcaac
       61 gacgagggag ccgacgatcg cgacccacct gaggcggcgt cggaggcgcg gcgattcggg
      121 gatccgaatc acgcgattca cggtaacaag aggtacgcac gtgcgcgaga cgagtattct
      181 tcgcgttgtg acgttcggcg caaaggtgct cgccgtcgcg atcctcgttg gcttcttcgt
      241 cgccggatcg tccgcagccc cgtcgaggta cgtctatcgc gacgtcgcgc tgattgccgt
      301 caccggccat ggcacggtga ccagcaagcc gcacggcatc gactgcccgg gcaggtgccg
      361 cacgctcttc gtccgcggca cgcatctgag tctccgcgcg gtggcggcgc ccggctggcg
      421 attcacgggc ttcaggagca cgttctgtaa cggtgcgcac gccacatgtg tcttcgacat
      481 ggtgagctca cacgactgcg tcggcggtgc ctgcccgacc ggggtgttcg gcgtccgcgc
      541 gcgcttcgtc cgggacagct gagaggaggc cgttcgtgct cgcaagcccg gtgacccagc
      601 gggtctgaaa cctcgatcac gccgaacgtg tttcggcagc gcttcgcgcc cacgagcgcc
      661 gcatcggcag caatccggac ggccattccg ctaggctgcg cctgcctcgg cggctcgcgc
      721 gcgtccttcg cctgaagccg caggtcaaac tcgcaccctc acgcctccag gagctctaga
      781 accgtgtcga cgaacggaaa cagcaccggc aagctgctcg agatcaaggg cgtcgtcgtc
      841 gacgccgtgt tcccggacaa gctccccgcg atctactcgg cgctgcgcat cccgcgcgcc
      901 gacggcccag atctgatcgc ggaggtgcag cagcacctcg gcgacgaccg cgtccgcgca
      961 gttgcgatgg atgcgaccga cggcatcgcg cgcggcaccg acgtgttcga taccggcgca
     1021 ccgatctcgg tgcccgtcgg ggaagtgacg ctcgggcgcg tgtggaacgt gctcggcgat
     1081 cccgtcgacg agaaggaagc ggcgggcaag gacgccgagc gctggtcgat ccaccgcgac
     1141 ccgcccgcct tcgccgacct ctcgccgaag gtggagatct tcgagaccgg catcaaggtc
     1201 atcgacctga tcgcgccgtt cgtgcgcggc ggcaagatcg gcctgttcgg gggcgccggc
     1261 gtggggaaga cggtgttgat ccaggagctg atccacaacg tcgcgcagga gca
//
LOCUS       DASUCJ010000998         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_423174, whole genome shotgun sequence.
ACCESSION   DASUCJ010000998 DASUCJ010000000
VERSION     DASUCJ010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423174"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFU90_11835"
     CDS             <1..796
                     /locus_tag="VFU90_11835"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5480521.1"
                     /translation="VGIAVGMAMEGLRPIAEIQFADFIYPAFNQIVGEAAKTRYRSNG
                     EYTCPLVIRTPYGGGVRGALSHSVSIEALFYHVPGLKIVAPAFPADVKGLLNSAIDDP
                     DPVLFLEHKKTYRLIKGEVPDGHYTIPIGKANVLKSGTQLSVVSYGLYVHWALDAARQ
                     LEDEGMSVEVIDLRSIRPMDKTAILQSVEKTRKLLIVHEDNKFGGIGAEISAMVAEEA
                     LFHLDAPIRRHCAPDVPAMGYALPLEEEYMSSPGEMAEAMREMVRF"
     gene            809..>1097
                     /locus_tag="VFU90_11840"
     CDS             809..>1097
                     /locus_tag="VFU90_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5480522.1"
                     /translation="MATTITMPQLGETVTEGTVERWLKKAGDSVEKYEAFVEVSTDKV
                     NSEVPAPVTGTIREILVQEGTTVATGTPIAVIDEVGAATAAHSASAPPQSKE"
BASE COUNT          236 a          337 c          340 g          184 t
ORIGIN      
        1 cgtcggcatc gcggtgggta tggcgatgga agggctgcgc ccgatcgccg aaatccagtt
       61 cgccgacttc atctacccgg cgttcaatca aatcgtcggc gaagcggcca agacgcgcta
      121 tcgctccaac ggcgagtaca cctgtccgct ggtgattcgc acgccttacg gcggcggcgt
      181 ccgcggggcg ctctcgcact cggtatcgat cgaggcgctc ttctaccacg tcccgggttt
      241 gaagatcgtc gcgccggcgt ttccggccga cgtcaagggc ctgctcaaca gcgccatcga
      301 cgatcccgat ccggtgctct tcctcgaaca taagaagacc taccggctga tcaaaggcga
      361 agtccccgac ggccactaca cgattccgat cggcaaggca aacgttctca agagcggcac
      421 gcagctgagc gtcgtttcct acgggctcta cgtccattgg gcgctcgacg cggcgcggca
      481 gctcgaagac gagggtatgt cggtcgaggt catcgatttg cgctcgatcc ggccgatgga
      541 caaaacggcg attctgcaaa gcgtggaaaa gacgcgcaag ctgctgatcg tccacgaaga
      601 caacaagttc ggcgggatcg gcgcggagat cagcgcgatg gtcgccgagg aggcgctctt
      661 tcatctcgac gcgccgatcc gccgtcactg cgctcccgac gttccggcga tgggctacgc
      721 gctgccgctg gaagaagaat acatgtcgtc gcccggcgag atggccgaag cgatgcgcga
      781 aatggtgagg ttctaggaaa cgaaaaatat ggctaccacg attacgatgc cgcagctcgg
      841 cgagaccgtg acggaaggca ccgtcgagcg ttggctcaag aaggccggcg acagcgttga
      901 aaaatacgaa gcgttcgtcg aagtatcgac cgacaaggtg aactccgaag tccccgcacc
      961 ggtcaccgga acgattcgcg agattctcgt gcaagaaggt acgaccgttg caaccggcac
     1021 accgattgcc gtcatcgacg aagtcggcgc tgctaccgcc gcgcatagcg cgagtgcgcc
     1081 gccccaatca aaagaag
//
LOCUS       DASUCJ010000999         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_41032, whole genome shotgun sequence.
ACCESSION   DASUCJ010000999 DASUCJ010000000
VERSION     DASUCJ010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41032"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFU90_11845"
     CDS             <1..601
                     /locus_tag="VFU90_11845"
                     /inference="COORDINATES: protein motif:HMM:NF023549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HEU5480523.1"
                     /translation="VFQGVAQNGIQLNVKARITVRSDLDRYVGGAGEPTIVARVGEGV
                     VSAVGAAVDHKEVLEYPDRISKAVLAKGLDAGTAFEIVSIDIADVDVGKNIGAELQTS
                     QAEADRRIAQAKAAERQYAALAAEQEQKAETQAMRAKVVEAEASIPQAIAEAFRSGHL
                     GVMDYYRLRNIQADTEMRASIGADGGDQAAPPAPPAPSK"
     gene            606..1019
                     /locus_tag="VFU90_11850"
     CDS             606..1019
                     /locus_tag="VFU90_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480524.1"
                     /translation="MRDIGALLWVAIVAIGVISSVVSNARRQAARTPVPPRRFPQAPS
                     QRPAAWAAPAAPQPPTVPLVTPRPAAPPPAAFAGTGVGVTPPPPPAPADIFPHPAELT
                     RARRPRRFAGRTALRRAVVAAEVLGKPRALRDEYP"
     gene            1064..2020
                     /locus_tag="VFU90_11855"
     CDS             1064..2020
                     /locus_tag="VFU90_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5480525.1"
                     /translation="MSVERTVDIHELPDRVRLFGQYDRNLNAIESSLGVSVRADGDHL
                     LLAGAQGDVERAERVVARVLEAAVRGAHVTPEDVALALADVRDNNGGAALPKTLLRAQ
                     RGSEVRARSAGQRAFVRAIEDHALTVGIGPAGTGKTFLAIVMAVRALKNREVARVILS
                     RPAVEAGENLGFLPGDLRDKVDPYMRPLFDALNELLDDGVVNRYIDRGTLEVAPLAYM
                     RGRTLSEAFVILDEAQNATADQLKMFLTRMGAASKMVVVGDATQIDLPPGNRSGLRDA
                     ANRLARVDDVAIVELDDTDVVRHPLVARIIRAYAPALPEERR"
     gene            2017..2484
                     /gene="ybeY"
                     /locus_tag="VFU90_11860"
     CDS             2017..2484
                     /gene="ybeY"
                     /locus_tag="VFU90_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5480526.1"
                     /translation="MIHYRNDVRGSGVDGRALVATARRLLDALGERGASLSLRLVGDR
                     AIRAINREHRGKDAATDVLSFSLREPNEDSAQRPVAERLLGDVVISVETARRQADGYD
                     ASLQDELYRLLIHGLLHVMGHDHEAAAQRRVMEREERRLAAAIGLRWPYEAAG"
BASE COUNT          440 a          896 c          914 g          388 t
ORIGIN      
        1 ggtcttccag ggcgtcgcgc aaaacggcat tcagctcaac gtcaaggcgc gcatcacggt
       61 gcgcagcgac ctcgaccgct acgtcggcgg cgccggcgag ccgacgatcg tcgcgcgcgt
      121 cggcgaaggc gtggtttccg cggtcggcgc cgccgtcgat cacaaagagg tgctggagta
      181 tcccgatcgc atcagcaaag cggtgcttgc gaaggggctg gatgcgggaa ccgcattcga
      241 aatcgtttcg atcgacatcg ccgacgtcga cgtcggcaag aacatcggcg ccgaactgca
      301 aacgagccaa gccgaagccg accggcgaat cgcgcaagcc aaagcggccg aacggcagta
      361 cgcggctttg gctgccgagc aagagcagaa ggccgagacg caggcaatgc gtgcgaaggt
      421 ggtcgaggcc gaagcttcga ttccgcaagc catcgccgaa gcgttccgga gcggccatct
      481 cggcgtgatg gattactacc ggctaaggaa catccaagcg gataccgaga tgcgggcctc
      541 gatcggcgcc gacggcggcg atcaggcggc tccgcccgcg ccgcccgcac cgtcgaagta
      601 gccgcgtgcg cgacatcggc gccctgctgt gggtcgcgat cgtggcgatc ggcgttatct
      661 cgagcgtcgt ctcgaacgcg cgccggcagg cggcacggac cccggttccc ccgcggcggt
      721 tcccacaggc gccgagtcag cgcccggctg cgtgggccgc tccggcggct ccccaacccc
      781 cgaccgtgcc cctcgtgacg ccgcggcccg ccgcgccgcc cccggcggcg ttcgccggca
      841 ccggcgtcgg ggtcaccccg ccgccgcctc ccgccccggc cgatattttc ccgcacccgg
      901 ccgagctgac gcgtgcgcgg cgtccgcgcc gcttcgccgg ccgcaccgcg ctgcggcgag
      961 cggtcgtcgc cgccgaggtc ttgggaaagc ctcgtgcgct gcgcgacgaa tacccgtaga
     1021 agtttttcga acttccaaaa cggagcccgc gtaaccgata gcgttgagcg tagaacgaac
     1081 cgtcgacatc cacgagcttc ccgaccgggt tcgcctattc gggcagtacg accggaacct
     1141 caacgcgatc gagtcgagtt tgggcgtgtc ggtgcgcgcc gacggcgacc acttgctgct
     1201 cgccggcgcg cagggcgacg tcgagcgcgc cgaacgcgtc gtcgcgcgcg tgctggaagc
     1261 ggccgtacgc ggagcgcacg tgacgccgga ggacgtcgcg ctcgcgctgg ccgacgtgcg
     1321 cgacaacaac ggcggggcgg cactccccaa gacgttgctg cgcgcccagc gcggctccga
     1381 ggtacgcgcg cgcagcgcgg ggcagcgggc gttcgtgcgc gccatcgagg atcacgcgct
     1441 gaccgtcggc atcggcccgg cgggaaccgg aaagacgttt ctcgcgatcg tcatggcggt
     1501 gcgcgcgcta aagaatcgcg aggtcgcgcg ggtgattctc tcgcgtccgg cggtggaagc
     1561 cggcgaaaat ctcgggtttc tccccggcga cttgcgcgac aaggtcgatc cgtacatgcg
     1621 gccgctgttc gacgcgctca acgagctcct cgacgacggc gtcgtcaacc ggtatatcga
     1681 ccgcgggacg ctcgaggtcg cgccgcttgc gtacatgcgc ggccggacct tgtcggaggc
     1741 gttcgtgatc ttggacgaag cgcaaaacgc gaccgccgat cagctcaaga tgtttctgac
     1801 gcgaatggga gccgcgtcga aaatggtcgt cgtcggcgac gcgacgcaga tcgacttgcc
     1861 gccgggcaac cgcagcggct tgcgcgatgc cgcgaaccgg ctggcgcgcg tcgacgacgt
     1921 ggcgatcgta gaactcgacg acaccgacgt cgttcgccac ccgctggtag cccggatcat
     1981 ccgggcgtac gctccggcgt tgcccgaaga acgccggtga ttcattatcg caacgacgtg
     2041 cggggaagcg gcgtcgacgg ccgggcgctg gtggcgaccg cacggcgatt gctcgacgca
     2101 ctcggcgaac gcggcgcttc gttatccctc cggctggtcg gcgaccgcgc gattcgcgcg
     2161 atcaatcgcg aacatcgcgg gaaggacgcg gcgaccgacg tgttgagttt ctcgctgcgc
     2221 gaaccgaacg aggattcggc gcaacggccc gtcgcggagc gtctgctcgg cgacgtcgtg
     2281 atttcggtcg aaaccgcgcg ccgccaagcc gacgggtacg acgcatcgtt gcaggacgag
     2341 ctgtatcggc tgctgattca cgggctgctg cacgtcatgg ggcacgatca cgaagcggcg
     2401 gcgcagcgcc gcgtgatgga gcgcgaagaa cggcgcttgg cggccgccat cggattacgc
     2461 tggccatacg aggctgcggg ctgatgcgcc accccgacga ggagcattcg aagccggcgc
     2521 cccactacgt gccgatcgca cagagcaagc tttggcgttc gttccatcac gctttcgaag
     2581 gaatcatcta cgcgacgcgc acccagccca acatgcgcgt tcacttggtg attgcggc
//
LOCUS       DASUCJ010001000         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1080464, whole genome shotgun sequence.
ACCESSION   DASUCJ010001000 DASUCJ010000000
VERSION     DASUCJ010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080464"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFU90_11865"
     CDS             <1..641
                     /locus_tag="VFU90_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEU5480527.1"
                     /translation="TFPPPCAYVANAGMPDVANSNGLPVDYRDNGVFFDRFQFDSLNQ
                     ASATPLNPIVNMTQDFISSHDGTSNTLLLSENLDAPSYANLGGGANNVFEAFHGFVWT
                     YSTNSASPPFEPQNFPYGTINGPRDLTNAANPAYYNNARPSSNHPGGVMVALCDGHTR
                     FLRSELDYGVYCLLMSPNGQGVQPAGGSWAGAPTPNNQLYLQSAPLSDQALD"
     gene            complement(700..>1111)
                     /locus_tag="VFU90_11870"
     CDS             complement(700..>1111)
                     /locus_tag="VFU90_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480528.1"
                     /translation="VRIDEPAGHLRPGQTAETEILLARRDDVLTIPVTAVVQQGDDVF
                     AWVQTGRSPERRPISLGAVNEKLAEISSGLDEGEALISNPRVQLAELFYSGDRGPRFD
                     VVQRFGLPAKDELHGRSAAPSAVKSQPETASGGG"
BASE COUNT          195 a          368 c          350 g          198 t
ORIGIN      
        1 acacgtttcc gcccccttgc gcctacgtgg cgaacgccgg catgcccgat gtcgcgaatt
       61 ccaacggcct gcccgtcgat tatcgcgaca atggcgtgtt cttcgatcgc ttccagttcg
      121 attcgttgaa ccaggcttcg gccacgccgc tgaatccgat cgtgaacatg acgcaagact
      181 tcatcagcag ccacgacggg acgtcgaaca cgctgttgtt gtcggaaaac ctcgatgcgc
      241 ccagctatgc gaacctcggc ggcggcgcga acaacgtctt cgaggcgttt cacggcttcg
      301 tctggaccta ttcgaccaac agcgcctcgc ccccgttcga gccgcagaat ttcccgtacg
      361 gcacgatcaa cggtcccagg gatctgacaa acgcggcgaa tccggcctac tacaacaacg
      421 cgcggccttc gagcaatcac ccgggcggcg tgatggtcgc gctctgcgac gggcacacga
      481 gattcctgcg ttccgaactg gattacggcg tgtattgttt gctgatgtct cccaacggcc
      541 aaggcgtaca gccggcgggc ggttcgtggg ccggcgcacc gactccgaac aaccagcttt
      601 atttgcagag tgcgccgctg agcgaccagg ccctcgattg agccggcgcc gccgatcggc
      661 ggtcggggat cgacgcttcg ccgcaagcag caagcgggtt cagccgccgc ccgaggcggt
      721 ctctggctgg ctcttgaccg cggagggtgc ggcggaacgc ccgtgcagtt cgtctttcgc
      781 cggcaggccg aaacgttgca cgacgtcgaa gcgcggcccg cggtcgcccg agtagaacaa
      841 ttcggcaagt tggacgcgag gattggaaat caaagcctcg ccttcgtcca agccggacga
      901 gatctcggcc agcttttcgt tgacggcgcc cagcgagatc ggacgccgct cgggcgaccg
      961 gccggtttgc acccaggcga acacgtcgtc gccctgctgc acgacggccg tgacgggaat
     1021 cgtcagtacg tcgtcgcggc gagccagcag gatttcggtc tcggccgttt ggccgggccg
     1081 cagatggccg gccggttcgt cgatccgcac g
//
LOCUS       DASUCJ010001001         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_438733, whole genome shotgun sequence.
ACCESSION   DASUCJ010001001 DASUCJ010000000
VERSION     DASUCJ010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438733"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..>1039)
                     /locus_tag="VFU90_11875"
     CDS             complement(164..>1039)
                     /locus_tag="VFU90_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU5480529.1"
                     /translation="AGTEFAVVKNVLDAGGAVVAVRYPGGAALSRRDFDALTETAKTL
                     GATGMIWIALGADGTKSSAAKFLSDATVAAIRAAAGAETGDALLLFAGARPLAYAVAG
                     KMRNEVGSRCGLRDPKKFAFCWVTDFPYLEIDEVTGKPAPAHHPFTAPAPGEWDLIET
                     DPTRMHAQHYDMVLNGYELGSGSIRIHQAQRQRQIFAMLGLTPEQVEDRFGFFLRALD
                     YGAPPHGGMALGIDRIVMLACGEDNIRDVIAFPKNQVARDLMMDAPSPVPKSSLRELH
                     VESTCEPGPAAAGDG"
BASE COUNT          163 a          374 c          339 g          163 t
ORIGIN      
        1 gccagcgtca gctcgcgcgt ttcgcagccg tgcagcggcg cgagatcggc gctcgcggcg
       61 gccgacaccg ccgtttcggc gtgccgcgcc gcgcagccgc ccgctcccag caggcacgcc
      121 gcctgcgcga gcgccgcgaa acgtccaaac cggcggcggg cgctcatccg tctccggcgg
      181 cagccggacc gggttcgcac gtgctctcga cgtgcagttc gcgcaacgag ctcttcggca
      241 ccggcgacgg cgcgtccatc atgagatcgc gtgcgacttg gtttttcgga aacgcgatga
      301 cgtcgcggat gttgtcctcg ccgcaggcga gcatgacgat ccggtcgatt cccagcgcca
      361 ttccgccgtg cggcggcgcg ccgtagtcga gcgcgcgcaa aaagaagccg aaccggtctt
      421 cgacctgctc cggggtcaac ccgagcatcg caaagatctg ccgctgccgc tgcgcctggt
      481 ggatgcggat cgagcccgag cccagctcgt aaccgttgag caccatgtcg tagtgttggg
      541 cgtgcattct cgtaggatcc gtctcgatca aatcccattc gccgggggcc ggtgcggtaa
      601 acgggtgatg cgccggggcg ggcttccccg tcacttcgtc gatttccaga tacggaaagt
      661 ccgtcaccca acagaaggca aacttctttg gatcgcgcag cccgcaacgg ctgccgactt
      721 cgttgcgcat ctttccggcg acggcgtacg cgagcgggcg cgcgccggca aacagcagca
      781 gcgcgtcgcc ggtctccgcg ccggcggccg cccgaatcgc ggcgacggtc gcgtcgctca
      841 ggaacttcgc ggccgacgac tttgtcccgt cggccccgag cgcgatccaa atcatccccg
      901 tcgcaccgag cgtcttggcg gtctcggtca acgcgtcgaa atcgcggcgc gacagcgccg
      961 cgccacccgg ataacggacc gcaacaacgg cgccgccggc atcgaggacg ttcttcacga
     1021 ccgcgaattc cgtcccggc
//
LOCUS       DASUCJ010001002         1465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_805661, whole genome shotgun sequence.
ACCESSION   DASUCJ010001002 DASUCJ010000000
VERSION     DASUCJ010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805661"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFU90_11880"
     CDS             <1..306
                     /locus_tag="VFU90_11880"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480530.1"
                     /translation="TIERVVLAAYHVLGCRDWSRIDVRLDAAGVPNVVEVNPLPGILP
                     DPADNSCFPKAARAAGMSYDELIQTCVRHAADRQGVSLPCSAARRRRTSVARAAASS"
     gene            411..>1465
                     /locus_tag="VFU90_11885"
     CDS             411..>1465
                     /locus_tag="VFU90_11885"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480531.1"
                     /translation="MKIALLFDGASALGKSADLAILETLEAIEAVLVADGHQVTRFPA
                     QPDGRWVERMRRGKFDLVFNMCEGLDGVAALEPAIISALEVLAVPFTGCSSWTASLCL
                     RKHVVNAFLERAGLPVPTFAVVHRGAPVPAVGFPAICKPAAEDASVGVEQRSVVRTNR
                     ALAARVEAMHEGWDEVLVQRYIDGREVNVGIVGNMVLPVSEIRFDGLPKRMWRIVSYR
                     SKWVPGSDEDVGAVPMCPADLPESLSDRITGVALDAWRLVGGTGYGRVDMRIDQDGQP
                     WILEVNANPDIARDAGLARMAAAAGLDYGALIRTVCDDAISRSALNVDERWALAQRLS
                     GVAPEVSDAAALQLVAVG"
BASE COUNT          238 a          472 c          496 g          259 t
ORIGIN      
        1 acgatcgaac gggtcgtgct cgccgcctac cacgtgctcg gctgtcgcga ttggtcgcga
       61 atcgatgtgc gactcgacgc ggcaggcgtg cccaacgtgg tcgaggtgaa tccgcttccc
      121 gggattcttc ccgatcccgc agacaactcg tgttttccca aggctgcgcg cgccgccggg
      181 atgagctacg acgagctcat tcagacgtgc gtgcgacatg ccgcggaccg gcagggcgtt
      241 tccctgccgt gtagcgctgc acgtcgacgc cggaccagcg tcgcgcgtgc ggcggcctcg
      301 agctgaggtc gcatgcgaga ccgcgtcgat tgcggaagtt gcgcgtcatc ccgacgcgcg
      361 ccatccggcg catctcggcg actgacgctc atcacgagtt ggaggagcgc atgaagatcg
      421 ccctgttatt cgatggagca tcggcgctcg gcaagtcggc agacctcgcg atcctcgaga
      481 cgctcgaagc gatcgaggcc gtgctcgttg ccgacggcca ccaggtgacg cgattccctg
      541 cccaaccgga cggacgctgg gtcgagcgta tgcgccgcgg caagttcgac ctcgtgttca
      601 acatgtgcga aggactcgac ggcgtcgccg ctctcgagcc agccatcatc tcggcgctcg
      661 aggtgctcgc ggtgccgttc accggctgct cgtcgtggac cgcgagcctc tgcctgcgca
      721 agcacgtggt gaacgcattc ctcgagcgag cgggcttgcc cgttccgaca ttcgcggtcg
      781 tgcatcgcgg cgcgcccgtg ccggccgtag gcttcccagc gatttgtaaa cccgccgccg
      841 aagatgcatc ggtcggcgtc gagcaacgtt cggtggttcg caccaatcgc gcgctcgccg
      901 cacgcgtcga agcgatgcac gaagggtggg acgaagtgct cgtccaacgc tacatcgacg
      961 gacgcgaagt gaacgtcggc atcgtcggca acatggtgct gccagtctcg gaaattcgtt
     1021 tcgacggatt gccgaagcgc atgtggcgca tcgtttcgta tcgatcgaag tgggtccccg
     1081 gcagcgatga agatgtcggc gccgtaccga tgtgccctgc ggacctgccc gagtcgctga
     1141 gcgacaggat cacgggagtc gcactcgacg cgtggcgact cgtcggggga accggctatg
     1201 gccgcgtcga catgcgcatc gatcaagacg gacaaccgtg gattctcgaa gtgaacgcga
     1261 accccgacat cgcgcgcgat gcgggactcg cgcgcatggc ggccgccgcc ggtctcgact
     1321 acggcgcgct gattcgcacg gtgtgcgacg acgcgatctc gcgcagcgct ttgaacgtcg
     1381 atgaacggtg ggcgttggcc caacggttgt ccggtgtcgc acccgaggtc agtgatgctg
     1441 cggcgctgca gctcgtcgcg gttgg
//
LOCUS       DASUCJ010001003         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_789976, whole genome shotgun sequence.
ACCESSION   DASUCJ010001003 DASUCJ010000000
VERSION     DASUCJ010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789976"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFU90_11890"
     CDS             complement(<1..514)
                     /locus_tag="VFU90_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480532.1"
                     /translation="MHRSVPPFSGATLVLSNARGERIELAPEGAAIVGIFVRDRRGAI
                     DCVSVAAGGSAGKTIGRYANRIGGGRFTLDGRTYRLLVNEGGNTLHGGPDGFSKRRWS
                     VAASQRDDEGRSLAAFTLFSPDGDQGFPGALNCSVTFTFGDDATLRIEYAATAEAPTV
                     INLTNHVYFNL"
     gene            543..1289
                     /locus_tag="VFU90_11895"
     CDS             543..1289
                     /locus_tag="VFU90_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5480533.1"
                     /translation="MKFAPVRRTLAILAVSASSIAATAPVPSDPAALPAMLGKSATHL
                     TFVSLGGDRRPLAVPGRPTVVIAFASWCEACAREMPRTLADYEKYRGRVTFLGIDYSD
                     PPPVAKQLVGKYGIPFPVESYVPGALTASKPARQTLTIPDNLTPQQLEGLRNKLPDAL
                     YQKAVAVYQARSLMTPEHFHAYEAWMGVYLADPKTVAAKMAAQKRAAPPLGLPHTFVI
                     DAGGVVADVLEGYLPSVDRVALALAKLGVR"
     gene            complement(1311..>2465)
                     /locus_tag="VFU90_11900"
     CDS             complement(1311..>2465)
                     /locus_tag="VFU90_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5480534.1"
                     /translation="ERQYDLNKAAELKYGRLLELEKQLKAEEERLASKQGVARLIKEE
                     VDEEDIAEVVSRWTHIPVSKLLEGEVQKLLQLEGELHQRVIGQDEAVTAVAEAVVRAR
                     SGLKDPNRPIGSIIFLGPTGVGKTELARALAEFLFDDERAMIRIDMSEYQEKHTVARL
                     LGAPPGYVGYEEGGQLTEAVRRRPYSVILFDEIEKAHPDVFNIFLQILDDGRLTDGQG
                     RTVDFKNTIVIMTSNIGSHRILEHRGTFRGADYAIMRATVLDELRQHFRPEFLNRVDE
                     TIVFHALTEEELEEIVDIQLQRLRKRLAERRITLVLSEAAKRHVVKVGYDPSYGARPL
                     KRTIQKELETPLGRKILGGEINDGDVVEVGFDEDRGELTFAVEAKQPVTA"
BASE COUNT          410 a          823 c          763 g          469 t
ORIGIN      
        1 caaggttgaa gtaaacgtga ttcgtgaggt tgatcacggt cggcgcctcg gcggtcgccg
       61 cgtactcgat gcgcagcgtc gcgtcgtcgc cgaacgtgaa ggtcaccgaa cagttcagcg
      121 cacccggaaa tccctggtcg ccgtccggac tgaacagcgt gaaggcggcg agcgaacgcc
      181 cttcatcgtc gcgctgcgag gctgcgacgc tccagcgccg cttcgagaaa ccgtcggggc
      241 cgccgtgcag cgtgttgccg ccttcgttga ccagcagacg gtacgttcgt ccgtcgagcg
      301 tgaagcggcc gccgccgatt cggttcgcat agcgcccgat cgttttgccg gcgctgccgc
      361 cggcggccac gctgacgcaa tcgatcgcgc cgcgccggtc gcgcacgaaa attcccacga
      421 ttgccgcacc ctccggtgcg agttcgatac gctcgccgcg tgcgttggac agcaccaggg
      481 tcgcgcccga gaacggtgga acggagcgat gcacggtcaa aacccttcgg aggaaagcgg
      541 gagtgaagtt cgcgccggtg agaaggactc ttgcgattct cgccgtctcg gcgtcgtcga
      601 tcgcagcgac ggcgcccgtt cccagcgatc cggccgcgtt gccggccatg ctggggaaat
      661 ccgcaacgca cctgacgttc gtctcgctcg ggggcgaccg gcggccgctt gccgtaccgg
      721 ggcgcccgac ggtcgtcatc gcatttgctt cgtggtgcga ggcgtgcgcg cgcgaaatgc
      781 cgcggacgct ggcggactac gaaaagtatc gcgggcgcgt tacctttctc ggcatcgact
      841 atagcgatcc gccgccggtc gcaaagcagc tggtcggcaa gtacggaatt ccgtttcccg
      901 tagaatcgta cgtgcccggc gcgttgacgg cgtcgaagcc cgcgcggcag acgctgacca
      961 tccccgacaa cctcaccccg cagcagctgg aaggtcttcg caacaaactg ccggacgcgc
     1021 tctatcaaaa agcggtcgcg gtgtaccaag cccgttcgtt gatgacgccg gaacattttc
     1081 acgcgtacga agcgtggatg ggcgtgtacc ttgccgatcc gaagaccgta gccgccaaga
     1141 tggccgccca gaaacgggct gcgccgccgc tcggcctgcc gcacacgttc gtgatcgacg
     1201 ccggcggggt cgtcgccgac gtgctcgagg gttacctgcc ctcggtggat cgcgtcgcgc
     1261 tcgcgctggc gaagctcggc gtccgttaaa gtcgctcgag acgctgcagg tcaagcggta
     1321 accggctgct tcgcctccac cgcaaaggtt agctcgccgc ggtcctcgtc gaagccgacc
     1381 tcgacgacgt cgccgtcgtt gatttctccg ccgaggattt tgcggccgag cggcgtctcg
     1441 agttcctttt gaatcgtgcg cttcaatggc cgcgcgccgt agctcggatc gtatcccacc
     1501 ttgacgacgt gccgtttcgc cgcttcggaa agtaccagcg taatgcggcg ctcggcgagc
     1561 cgtttacgca gccgctgcag ctgaatgtcg acgatctcct ccagctcctc ctcggtgagc
     1621 gcgtgaaaga cgatcgtttc gtcgacgcgg ttgagaaact ccggccggaa atgctggcgc
     1681 agctcgtcga gcacggtcgc gcgcatgatc gcgtaatcgg cgccgcggaa cgttccgcga
     1741 tgttcgagaa tgcgatggct tccgatattc gacgtcatga tgacgatcgt gttcttgaaa
     1801 tcaacggtcc gcccctgacc gtcggtcagc cgcccgtcgt cgagtatctg caggaagatg
     1861 ttgaagacgt ccggatgcgc cttttcgatc tcgtcgaaca gaatgacgct gtacggccgg
     1921 cggcgcaccg cttcggtgag ttgaccgcct tcctcgtagc cgacgtatcc cggcggcgct
     1981 ccgagcagcc gcgcgacggt gtgcttttcc tgatactccg acatgtcgat gcggatcatc
     2041 gcccgctcgt cgtcgaagag aaactcggcc agcgcccgcg ccaactccgt tttgccgacc
     2101 ccggtcggac ccaggaagat aatcgagccg atgggccgat tcggatcttt cagtcccgaa
     2161 cgcgctcgca ccaccgcctc ggcgaccgcc gtcaccgcct cgtcctggcc gatgacgcgc
     2221 tggtgcaact ctccttccag ctggagcaac ttctgtacct cgccctccag cagcttggaa
     2281 accggaatgt gcgtccaacg gctgacgacc tcggcgatgt cctcctcatc cacttcctcc
     2341 ttgatgagcc gggcgacgcc ctgcttcgag gccaggcgtt cctcctcggc cttgagttgc
     2401 ttttccaact ccagcaatcg gccgtacttc aattcggcgg ccttgttcaa atcatactgc
     2461 cgttc
//
LOCUS       DASUCJ010001004         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1019300, whole genome shotgun sequence.
ACCESSION   DASUCJ010001004 DASUCJ010000000
VERSION     DASUCJ010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019300"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..493
                     /locus_tag="VFU90_11905"
     CDS             293..493
                     /locus_tag="VFU90_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480535.1"
                     /translation="MEVSLVNTEDIKNRLNHALHRHPKATEDEVEAITAIVLAIVGEV
                     TVELAAVIGDLAARVDALEARA"
BASE COUNT          171 a          348 c          328 g          200 t
ORIGIN      
        1 agccgtcgtc gtgcaaccac agcattcccc cctggtcacg aacacgttcg ctgcacgtat
       61 cggcaaccgc gacgctcgtt ctggaggcac cgacgcttcc tttaccgttc tcgcatgtgc
      121 gcaacgacca caacaacacc agcggccgcg tttggtcaca ccgtgcacca gcgacgcgcg
      181 cgcggctacg gctcggtcgc gctcagcctc gaccgaacgg cggcacagcc tccgctaccg
      241 gagggcgcgt ccgtaaacct tgatctcgtt tacagtccgg gccggatcat tcgtggaggt
      301 ctcactcgtg aacaccgaag acatcaagaa ccgcctcaac cacgcgttgc accgccatcc
      361 gaaagcgacc gaagacgaag tcgaagcgat cacggcgatc gtgctcgcga tcgtcggcga
      421 agtcaccgtc gaactcgccg ccgtgatcgg cgacctcgcg gcacgcgtcg acgcgctcga
      481 agcccgcgcg tgaaccccgg ccgccgtcta cgacgagaag gcagcaggcc gcgcacgtat
      541 tcgcaaccct gcgccacgcc gaaggcaggg ccgtcgccgt aggcgacgaa cgggtgcgaa
      601 tgccaggcgc gggtccagcg cgcgcgaccc actcgcgtat gacgaccttg gtctcgtgcc
      661 cgacgtttgg ataaacggct ggacctgtca ggcgatccgg cgtatgaatg cccgttctgc
      721 gctcgccaca accgccaccg atcgaaccct gtttgtgtca agaggctggt ttcgggcgcg
      781 gtgggttgtg ctcgtgactt ccgggttgat tcgttctagc gttgtggttc gggtccggga
      841 atggcctctc cgaagtgccg gcgttcaggg atctgccggc cgcgctggat ttcagagtcg
      901 cccctgattg tcctcccggg cccgtttcgt cttagttgtc ggcgtcggcg cgtagtcgtt
      961 ggtagacggc gtcgctgatg cgtcgtttga ggcagcgcat cgcttccttg cggctcttcc
     1021 cgtcggcttg tttggtgagg tagtagg
//
LOCUS       DASUCJ010001005         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1095901, whole genome shotgun sequence.
ACCESSION   DASUCJ010001005 DASUCJ010000000
VERSION     DASUCJ010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095901"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="hpnH"
                     /locus_tag="VFU90_11910"
     CDS             <1..586
                     /gene="hpnH"
                     /locus_tag="VFU90_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEU5480536.1"
                     /translation="VCRDGVFDKAVEAIKDAKERGFRVYTNTTFFDQDGPESIRAVLD
                     YLNDELKVDMMQISPAYAYEKAPDQEHFLGVKRTHDVFSKAFAGGKRKKWRLNHSPMY
                     LDFLEGKVDFECTPWGIPCYTVFGWQRPCYLMSKEGYAKTYRELLEETDWSKYGRGKH
                     ESCENCMAHCGYEPTAVLRTTGSLKESIRAAFGS"
     gene            complement(665..>1028)
                     /locus_tag="VFU90_11915"
     CDS             complement(665..>1028)
                     /locus_tag="VFU90_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480537.1"
                     /translation="SRSAVARVPDRCRTRRKNGALDTRQMRGRQQTLRPDDVYGVNRV
                     TIPRCKGVQCCGVHDVAAPVERPRELAGPAHIPNDEFEAGNARLRRHGELPENVRPVA
                     LRVAHQSAHGVTGRQQLA"
BASE COUNT          182 a          316 c          348 g          182 t
ORIGIN      
        1 ggtctgccgc gacggggtct tcgataaagc ggtcgaggcg atcaaggatg cgaaggagcg
       61 cggcttccgg gtctacacga acacgacgtt cttcgatcaa gacggtcccg aatcgattcg
      121 cgccgtcctc gactacctca acgacgagct gaaagtcgac atgatgcaaa tctcgccggc
      181 ctacgcttac gagaaggctc ccgaccagga gcacttcctc ggcgtcaagc gcacgcacga
      241 cgtctttagt aaggctttcg ccggcggtaa gcgcaagaag tggcggctca atcacagccc
      301 gatgtacctg gacttcttgg aaggaaaggt cgatttcgag tgcacgccct gggggattcc
      361 gtgctacacg gttttcggtt ggcagcgccc gtgctatttg atgagcaagg aagggtacgc
      421 gaagacgtat cgcgagctgc tcgaggagac cgactggtcg aaatacgggc gcgggaagca
      481 cgagtcgtgc gagaattgca tggcccattg cggatacgaa cccaccgcgg tactgcgcac
      541 gacggggtcg ctgaaggaat cgatccgggc ggccttcggc agctagggac tcccgccgat
      601 tagcgccgag ccggactcgc gcatcttcct gaccggagcg tcgggcttcg tcggcgccca
      661 cgtcttacgc gagctgctgt cggccggtta ccccgtgcgc gctttggtgc gcgacgcgga
      721 gcgcgaccgg ccgcacgttc tcgggcaact cgccgtgccg ccgcaggcga gcgttgccgg
      781 cctcgaactc gtcgttgggg atatgcgccg gcccggcgag ctcgcgcggg cgctcaacgg
      841 gtgccgctac gtcgtgcacg ccgcagcact gtactccttt gcaccgcggg atcgtgacgc
      901 gattcacgcc gtaaacgtcg tcgggacgga gggtttgttg gcggccgcgc atctggcggg
      961 tgtcgagcgc gccgttctta cgtcgagttc ggcaacgatc aggcacgcgc gcgacggcag
     1021 accggctg
//
LOCUS       DASUCJ010001006         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_407544, whole genome shotgun sequence.
ACCESSION   DASUCJ010001006 DASUCJ010000000
VERSION     DASUCJ010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407544"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFU90_11920"
     CDS             complement(<1..455)
                     /locus_tag="VFU90_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480538.1"
                     /translation="MTVAKDATAADATTTADASEAQDASFVADGDTDATTHVAACLAK
                     VDAGAMCNMLEPQGPTIVATCSNASAPQPMGGTIIDGTYVLQSMTIYGTCPPNGEIGR
                     FIWLVCGDAWTSAQETPTSPANPDAGVTVIHVNASVAASGSMLTINQTCT"
     gene            817..>1050
                     /locus_tag="VFU90_11925"
     CDS             817..>1050
                     /locus_tag="VFU90_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480539.1"
                     /translation="MTAHRMLESFAARFDVSALEEDEATMVAVDAAGTIAWTNRAWAE
                     FAKANGGETVLARFTVGTSYLDGISGQTRSFYES"
BASE COUNT          183 a          326 c          347 g          194 t
ORIGIN      
        1 gtgcaggtct ggttgatggt cagcatcgat ccggaagccg cgacactcgc gttgacgtgg
       61 atgacggtaa cgccggcatc gggattcgcg ggactcgtcg gagtctcctg ggcgctcgtc
      121 cacgcatcgc cgcatacgag ccatatgaat ctaccgattt cgccgttcgg cgggcatgtc
      181 ccgtagatcg tcatcgactg gaggacgtac gtaccgtcga tgatcgtccc gcccatcggc
      241 tgcggagccg aggcgttcga gcacgtcgcg acaatcgtcg gaccctgcgg ttcgagcata
      301 ttgcacatcg cccctgcatc gaccttcgcg aggcaagcgg cgacgtgcgt cgtggcgtcc
      361 gtatccccgt ccgctacgaa gctcgcgtct tgcgcctcac ttgcgtcggc cgtagtcgtc
      421 gcgtcggccg ctgttgcgtc ctttgccacg gtcacgtcgt tcggctcgct tgcgtcggtc
      481 ggctccgtat ccgaagtgcc gtccgcggtc cccgcgtcgt gcgttgacgg cccggcgtcg
      541 ctcgcaccga gaggcacatg ggaagagtcg ccgctactgc cgcacgctgc aaggaccgta
      601 gccgccaggc cgcaccacaa aggattcgaa aagtcgccgt tcataggggc gcagagtacc
      661 ggaagcgtca ccgcgggaag gccaactggt tacaagcggg gaaacggatc ggcgcaaaaa
      721 attcgtgctg acgcagcttt cgccaatcga cggtggcgcg gagcgccgga catgggccga
      781 atgtcatgcg ccgaatgagg gtggcccggc atttgaatga ccgctcatcg catgctggag
      841 tcgttcgcgg cccgattcga cgtttcggcg ctcgaggaag acgaagccac gatggtggcc
      901 gtcgacgcgg cgggaacgat tgcttggacc aatcgggcgt gggccgagtt cgcgaaggcc
      961 aacggcggag aaacggtgct tgcgcgattc acagtcggca cctcctatct cgacggcatc
     1021 tctggtcaga cgcgaagctt ttacgagagc
//
LOCUS       DASUCJ010001007         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_239679, whole genome shotgun sequence.
ACCESSION   DASUCJ010001007 DASUCJ010000000
VERSION     DASUCJ010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239679"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VFU90_11930"
     CDS             complement(<1..1153)
                     /locus_tag="VFU90_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU5480540.1"
                     /translation="MPTVVDRGLEGVVVGSTQLSNVEGVAGRLTYRGYDIDDLAPNAT
                     FEEIVHLLLYGHLPNRHELETLKRELGSRRSLPEPLITSMQNVPKTAWPMDVLRTVVS
                     GLGLFSPVNAHGEHLSDVHTAIELIAKMPTIVAAWDRIRRGLDPVDPRANLSQAANFL
                     YMRSGKLPHAIEADALDTYFVLLADHSYNASTFAARVAASTRADVYASVTAALATLQG
                     DLHGGAASASYHALTEIKEPENAEGFVREALARNERIMGMGHREYKVRDPRARHLEAM
                     AKKLSEHEGNSRWYQIAHRLEEASRKVLQEKKPDNTIYANVDFYTAPVLADLGIPGDE
                     FTCLFACGRIAGWTGHILEQLADNRLIRPQATYDGPAPGPYVPIEKRDVN"
     gene            1322..1756
                     /locus_tag="VFU90_11935"
     CDS             1322..1756
                     /locus_tag="VFU90_11935"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEU5480541.1"
                     /translation="MDALDLTKQAPRAPRELLPGLELLMMARTVDKLRATLPGGNLGN
                     YQIPGFSARLLEALGIGEASLRDAVAAARSDDDVVAWIRRNSDPARYAQVNVALESRK
                     VADRLDDPAFVAKYPIIQRLPPEMPLLDMLVADDRLAFEPAS"
     gene            complement(1753..3144)
                     /locus_tag="VFU90_11940"
     CDS             complement(1753..3144)
                     /locus_tag="VFU90_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480542.1"
                     /translation="MEFDCRCPHAPYSHAIFIQAQRALARRSFLIGAAATGAALLAPW
                     SRARAAGSAVVLKAARLFTGQTMQTPGVLVIAGDRIASLHEGDAGSAATTIDLGDATI
                     MPGLIDCHTHVSAFVVPSYYLTTMFPKYRVADNVPEATIYSVRNAQTMLKNGFTTIRD
                     VGGGAGIDLAVRDAVNNGAIVGPHIFAAGQALSITGGHGDSNDVPGWVDEDPSVRIGA
                     ISYGPYGFRELVREHVKLHVDVIKILATGGVLSYGDVWDVPQFNLDEVQAVVDESSKF
                     GRKVAAHAHGDKGIAIAVEGGVNSVEHGTGVSEKTLQSMQQRGTNLVPTIWALDSILQ
                     PGNPNHIPPTSLQKAEYAAQLRNEGMHRAIASSVTIAYGTDAGVFPHAQNNKDFGLLV
                     GLGMRPIDVLRSATFGAAELIGATDRGRLVPGKLADVVAFNGNPLAQISVMERQPALV
                     MLAGKSVDLARLP"
     gene            3217..>3885
                     /locus_tag="VFU90_11945"
     CDS             3217..>3885
                     /locus_tag="VFU90_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5480543.1"
                     /translation="MSEEIAVSTDSGVGTIRIDRPAKKNALTTAMYGAMADALEAFGT
                     DDSVRIVKILGGADFSAGNDLQDFTGASASGDTAAFVAVIRFLERLCDFPKPVVAGVR
                     GNAIGIGTTMLLHAAVAVAATSARFRLPFAQLGLVPEAGSTLLLPLAVGRMRATWLLL
                     AGDFFGADEALAMGLVTKTADDAQVDAAAGEIAARLAALPAEALRETKRLIREPISDA
                     VRTQM"
BASE COUNT          630 a         1303 c         1283 g          669 t
ORIGIN      
        1 cgttgacgtc gcgtttttcg atcggaacgt acggaccggg cgccggcccg tcgtacgtcg
       61 cttgcggacg gatgagccgg ttgtccgcga gctgctctaa gatgtgtccg gtccagccgg
      121 cgatccggcc gcaagcaaac aaacaggtga actcgtcgcc gggaatcccg agatcggcca
      181 gcaccggcgc cgtgtagaaa tcgacgttag cgtagatcgt gttgtcgggt tttttctcct
      241 gcagcacttt gcggctggcc tcttccagcc gatgcgcgat ttgataccat cggctgttgc
      301 cttcgtgctc ggaaagcttt ttggccatcg cttccaaatg ccgggcgcgc ggatcgcgga
      361 ctttgtattc gcgatgcccc atgcccatga tccgctcgtt gcgcgccagc gcctcgcgaa
      421 cgaagccctc ggcgttttcc ggctccttga tctcggtcag cgcgtgatac gacgcgctcg
      481 ccgcaccgcc gtgcagatcg ccttggagcg tcgccaacgc ggccgttacc gacgcgtaaa
      541 cgtcggcacg cgtcgaagcc gcaacccggg ccgcaaacgt cgacgcgttg tacgagtgat
      601 cggcgagcag cacgaaatac gtgtcgagcg cgtcggcttc gattgcgtgc ggcagcttgc
      661 ccgaacgcat gtagaggaag ttcgccgcct gcgacaggtt ggcgcgagga tcgaccggat
      721 ccagcccgcg gcgaatccgg tcccacgcgg cgacgatcgt cggcatcttg gcgatcagct
      781 cgatcgcggt gtgcacgtcg gagaggtgct cgccatgcgc gttgaccggc gagaacagcc
      841 cgagcccgct gacgaccgtg cgcagcacgt ccatcggcca cgccgttttc ggcacgttct
      901 gcatcgaggt gatcagcggt tccgggagcg agcggcgcga gcccagctcg cgcttgaggg
      961 tctccagctc gtgccggttg ggcagatggc cgtacagcag caggtggacg atctcttcga
     1021 acgtcgcatt gggagcgagg tcgtcgatgt cgtagccgcg ataggtcagc cggccggcga
     1081 ccccctcgac gttactgagc tgcgtagacc cgacgacgac gccctcgagg ccgcggtcga
     1141 caacggttgg caaggatccc tccaaatacg gcgttccgcc catgcgcgga gcaggcccaa
     1201 ggtttttcgg cgggcgcggg gtcgcctgcc gccccccggc cggcgctttc cagaacaccg
     1261 ttcggcggac ggccgtctca ctccgagccg gaagccggcg tgcgaccgcg aagccgccgg
     1321 catggacgct ctcgatctta ccaagcaggc accgcgcgct ccgcgcgagc tgcttcccgg
     1381 tctcgaactt ctgatgatgg cgcgaaccgt cgacaaactg cgcgcgacac tccccggcgg
     1441 caacctcggg aactatcaga tccccggctt cagcgcgcgg ctgctcgaag cgctcggcat
     1501 cggcgaagcc tcgctgcgcg acgcggtcgc cgcggcgcgc agcgacgacg acgtcgtcgc
     1561 ctggatccgc cgaaactccg atccggcgcg ctacgcgcag gtaaacgtag cgctcgagtc
     1621 gcgcaaggtc gcggaccggc tcgacgatcc ggcgttcgtt gccaagtatc cgatcatcca
     1681 acgcctgccg ccggagatgc cgcttctcga catgctcgtc gccgacgacc gcttggcgtt
     1741 cgagccggct tcctaaggaa gacgcgcgag gtcgacggat tttccggcca gcatgacgag
     1801 cgcgggctgg cgttccatca ccgagatctg ggccagcggg ttgccgttga aggcgaccac
     1861 gtcggccagc ttcccgggga cgaggcgtcc gcgatcggtc gctccgatca gctcggccgc
     1921 gccgaacgtg gcggagcgca agacgtcgat cggccgcatc ccgaggccga ccagcagacc
     1981 gaaatccttg ttgttctgcg cgtgcgggaa cacgccggca tcggtgccgt aggcaatcgt
     2041 aacgctcgag gcgatcgcac gatgcatccc ttcgttgcgc agctgcgccg cgtactcggc
     2101 cttttgcagc gacgtcggcg gaatgtgatt cggattgccg ggctggagga tcgaatccag
     2161 cgcccagatc gtgggtacga gattggtccc gcgctgctgc atcgactgca gcgtcttctc
     2221 gctcacgccg gtaccgtgtt cgaccgaatt gacgccgcct tcgaccgcga tcgcgatccc
     2281 cttgtcgccg tgcgcgtgcg cggcgacctt ccggccgaat ttggacgatt cgtcgacgac
     2341 cgcttgcacc tcgtcgaggt tgaactgcgg cacgtcccac acgtcgccgt acgaaagcac
     2401 tccgccggtg gcgagaatct tgatgacgtc gacgtgcagc ttcacgtgct cgcgcaccag
     2461 ctcccgaaag ccgtagggtc cgtacgaaat cgccccgatg cggaccgaag gatcttcgtc
     2521 gacccagccg ggaacgtcgt tcgaatcgcc gtgcccgccg gtaatcgaga gcgcctgccc
     2581 ggccgcaaag atgtgcggac ccacgattgc gccgttattc acggcgtcgc gtacggcgag
     2641 gtcgatgccg gcgccgccgc ccacgtcgcg gatggtggtg aacccgttct tgagcatcgt
     2701 ctgtgcgttg cgcacgctgt agatcgttgc ttcgggaacg ttgtcggcta cccggtattt
     2761 cgggaacatc gtcgtgaggt agtacgaagg gacgacgaac gccgaaacgt gcgtatggca
     2821 gtcgatcaaa ccgggcatga tcgtcgcgtc gcccaaatcg atcgtggtcg ccgcgctccc
     2881 ggcgtcgcct tcgtggagcg acgcgattcg gtcgcccgca atcaccagca cccccggcgt
     2941 ctgcatcgtc tgtccggtaa acagccgcgc cgccttgaga acgacggcgc ttcccgccgc
     3001 acgcgcgcgc gaccacggcg cgagcagcgc ggcgccggtt gcggcggctc cgatcagaaa
     3061 cgagcggcgc gcgagcgcgc gctgcgcttg gatgaagatt gcgtgcgaat acggcgcgtg
     3121 tggacaacgg caatcgaatt ccatgcgccg tgagttcgcc gccgcggcgc gtccgtcctg
     3181 agggtgacgg taagcaaccg tcgaatagct cgaaccgtgt cagaagagat cgccgtttcg
     3241 acggattcgg gcgtcggcac gattcggatc gaccgccccg cgaagaagaa cgcgctcacg
     3301 accgcgatgt acggtgcgat ggccgacgcg ctcgaagcgt tcggcaccga cgactcggtt
     3361 cgcatcgtca aaatccttgg cggcgcagac ttctccgccg gcaacgattt gcaagacttt
     3421 accggcgcgt cggcatccgg cgacaccgcg gcgttcgtcg cggtgatccg gtttctcgag
     3481 cggctctgcg atttccccaa gccggtcgtc gccggcgttc gcggcaacgc gatcggtatc
     3541 ggcacgacca tgctgctgca cgccgccgtc gcggtcgccg caacgagcgc gcgcttccgg
     3601 ctcccgttcg ctcagctcgg gctggtaccg gaagccggct cgacgctgct cctgccgctg
     3661 gcggtcggac ggatgcgtgc gacctggctg ctgctggcgg gcgatttctt cggcgccgac
     3721 gaggcgctgg cgatgggact ggtaacgaaa acggccgacg acgcgcaagt cgacgccgcc
     3781 gccggcgaga tcgcagcgcg gctcgccgcg ctgcccgcag aagccttgcg cgagaccaag
     3841 cggctgatcc gcgagccgat ctccgacgcg gttcgcacgc agatg
//
LOCUS       DASUCJ010001008         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_25968, whole genome shotgun sequence.
ACCESSION   DASUCJ010001008 DASUCJ010000000
VERSION     DASUCJ010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25968"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFU90_11950"
     CDS             complement(<1..792)
                     /locus_tag="VFU90_11950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5480544.1"
                     /translation="MLRLSIVIPVYDNWWLTARALRALDRLRGDSPAFETIVVDNASR
                     DGTPQGIAAFPWVRYERMDVNRNFAGACNAGAKLAQAPLVLFLNNDAYPLDDALSPLA
                     AAFEREETAIAGGALFFEDGVTQCAGFVMLPNAHWHYSCRNLPATLAGVTLARDAPAV
                     SGAAMAVRRDWFLSEGGFDESYCNGFEDVDLCMRARERGRTIAYVAQARFAHYEGASA
                     GRYDREADNERRFYRRWAPALAGIPRVARGEVGAIAIRTAPALSPL"
     gene            complement(797..1237)
                     /locus_tag="VFU90_11955"
     CDS             complement(797..1237)
                     /locus_tag="VFU90_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEU5480545.1"
                     /translation="MSRAFVKDGDDRPERPVPRPVSAGPNYVTPAGLEQLREALAHAR
                     ASNDERNAVYYEERIESAEVIESHPGPSGAIGFGSTVAVREANGALVRMRIVGEDEAD
                     PKRGTIAWNSPYARALMDHRRGDRVVVQRPAGPAAVVVESVDNA"
     gene            complement(1234..2226)
                     /locus_tag="VFU90_11960"
     CDS             complement(1234..2226)
                     /locus_tag="VFU90_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5480546.1"
                     /translation="MLAAVRYASDPRGRIRIEDAREPEPGAGEVLLRVRACGVCRTDL
                     HVADAELPPHRDRVVPGHQIVGEVVAVGAGVGETLAGTRAGVSWLGGTDGTCAQCLAG
                     RENLCDAPTYTGYDRDGGYAQFATARADFTIPLPPELDDRRAAPLLCAGIIGFRSLRV
                     AGVEPGERVGLYGFGASAHLALQVLHAWNCEAYVATRGEAHRAFAKRLGAAWTGDALD
                     VPPVRLDRAVTFAPAGDVVIAALSALRKGGVVAINAIHLDRMPAFDYDSLLWGERQIR
                     SVTNATRTDARDFLELAARAPISAQTTVFPLREAPDALDAVRNDRLDGAAVLIP"
     gene            complement(2245..>3083)
                     /locus_tag="VFU90_11965"
     CDS             complement(2245..>3083)
                     /locus_tag="VFU90_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEU5480547.1"
                     /translation="GFAVLPSLVLIAGMIVMPESPRYLFKIGHDSRARDVLNRIAETP
                     EDSVQEERAIHEALAVKSAGFAVFKDRSVALALFVGVTLAVLQQVTGINTVIYYGPRI
                     FEMAGIASASGSILAESIVGVVNVVMTLIAIFFVDRIGRKPLLYAGLTGMFVALAALA
                     IAFAQPPGATGSLGAIALVSMMVYVGCFAFSLGPIVWLLISEIFPLPARGIGMSISTL
                     ANWVGNFLVSQFFLTMIDKLGRPATFWLYAVLCVVTIGFVRAMVPETKRELLERIRVE
                     AV"
BASE COUNT          546 a         1082 c         1007 g          448 t
ORIGIN      
        1 gagcggtgag agcgccggcg ccgtccggat cgcgattgcg ccgacttcac cgcgtgcgac
       61 tcgcgggatg ccggccaacg cgggcgccca gcggcggtaa aaacggcgct cgttgtcggc
      121 ttcacgatcg taacgcccgg ccgaggcgcc ttcgtagtgc gcgaagcgcg cctgcgcgac
      181 gtacgcgatc gtgcgcccgc gctctcgcgc gcgcatgcac aagtcgacgt cttcgaatcc
      241 gttgcagtac gactcgtcga atccgccttc gctcaaaaac cagtcgcgcc ggacggccat
      301 agccgctccc gacacggccg gcgcgtcgcg cgcgagcgtc acccccgcca gcgttgcggg
      361 gaggttccgg cacgaataat gccagtgcgc gttgggcagc atcacgaagc ccgcgcactg
      421 cgtcacgccg tcttcgaaga acagcgctcc gccggcgata gccgtctctt cgcgctcgaa
      481 ggcggccgcc agcgggctca gcgcatcgtc cagtggatag gcgtcgttgt tcaagaacag
      541 cacgagcggc gcttgcgcca gtttcgcacc cgcattgcag gcgccggcaa agttgcggtt
      601 gacatccatc cgttcgtatc ggacccacgg gaacgccgcg atgccttgcg gcgtgccgtc
      661 gcgcgaggcg ttgtcgacga cgatcgtttc gaacgccggc gaatcgccgc gcagccgatc
      721 gagcgcgcgc agcgcgcgcg ccgtcagcca ccagttatcg tagaccggga tgacgatgct
      781 gagccgaagc acgcgcttac gcgttgtcga ccgactcgac gacgaccgcc gccggcccgg
      841 cgggccgctg cacgacgacg cggtcgccgc gacgatggtc catcaacgcg cgtgcatacg
      901 gcgagttcca agcgatcgtt ccgcgcttcg gatcggcctc gtcttcgccg acgatgcgca
      961 tccggaccag cgccccattg gcctcgcgta ccgcgacggt cgaaccaaaa ccaatcgctc
     1021 cggacggacc ggggtgcgat tcgatcactt cggcgctctc gatgcgttcc tcgtaataga
     1081 cggcgttacg ctcgtcgttg ctcgcccgcg cgtgcgcgag cgcttcccgc aactgctcga
     1141 gtcccgccgg cgtcacgtag ttcggaccgg cgctcaccgg gcgcggtacc ggacgctcgg
     1201 gacggtcgtc gccgtccttg acgaacgcgc ggctcacgga atcagaaccg ccgcaccgtc
     1261 gaggcgatcg ttgcgcacgg cgtcgagcgc gtccggcgcc tcgcgcaacg ggaagaccgt
     1321 ggtctgcgcg gagatcggcg cgcgcgcggc cagctccagg aaatcgcgcg cgtcggttcg
     1381 cgtggcgttg gtcacgctgc ggatctggcg ctcgccccag agcagcgagt cgtagtcgaa
     1441 cgccggcatg cgatccaagt gaatggcgtt gatggccacg actccgcctt tgcgcagcgc
     1501 gctcaatgcg gcgatcacga cgtcgcccgc gggcgcgaag gtgacggcgc ggtcgaggcg
     1561 gacgggcggc acgtccagag catcgccggt ccatgccgct cccagccgct tggcaaaggc
     1621 gcgatgcgct tcgccgcgcg tcgcaacgta cgcctcgcaa ttccaggcgt gcagcacctg
     1681 cagcgccaag tgcgcggagg ccccgaagcc gtacaaaccg actcgctcgc ccggctcgac
     1741 gcccgcgacg cgcaacgagc gaaacccgat gatgccggcg cacaacagcg gggcggcacg
     1801 ccggtcgtcg agctccggcg gcagcggaat cgtgaagtcg gcgcgcgcgg ttgcgaactg
     1861 cgcgtagccg ccgtcgcggt cgtacccggt gtaggtcggt gcgtcgcaca aattctcacg
     1921 accggcgaga cactgcgcgc acgtcccatc ggttccgccc agccacgaaa cgccggcccg
     1981 cgtaccggcg agcgtttcgc cgaccccggc gcccaccgcc acgacctcgc ccacgatttg
     2041 atgccccggc accacgcggt cgcgatgcgg cgggagctcg gcgtcggcga cgtgcaaatc
     2101 ggtccggcaa acgccgcacg cgcgtacgcg cagcagcacc tcgccagcgc ctggttcggg
     2161 ctcgcgggcg tcttcgatcc gtattcgccc gcgagggtcg ctcgcgtagc gcacagccgc
     2221 caacatttgg gaaatctaaa gacctcagac cgcctcgacg cggatgcgct cgagcagctc
     2281 gcgcttcgtc tcggggacca tcgcccgaac gaaaccgatc gttacgacgc acagcacggc
     2341 gtacagccaa aacgtcgccg gccggccgag tttgtcgatc atcgtcagga aaaactgcga
     2401 gacgagaaag ttgccgaccc agttggccag cgtcgaaatc gacatcccga tgccgcgcgc
     2461 cggcaatgga aatatctccg agatcaacag ccacacgatc ggcccgagac taaacgcaaa
     2521 gcagccgacg tacaccatca tgctgacgag cgcaattgca cccaacgagc cggtggcgcc
     2581 cggcggctgc gcgaacgcga tcgcgagcgc cgccagcgcg acgaacatcc ccgtaagccc
     2641 ggcgtagagc agcggctttc ggccgatccg gtcgacgaaa aagatcgcga tcagcgtcat
     2701 cacgacgttg accacgccga cgatcgactc ggcgagaatc gatccggagg ccgaagcgat
     2761 gccggccatt tcgaagatac gaggcccgta gtagatgacg gtgttgatgc cggtgacctg
     2821 ctgcagtacc gcgagggtta cgccgacgaa cagcgcgagc gcgaccgagc gatccttgaa
     2881 aacggcgaaa cccgcgctct tgaccgcgag cgcttcgtga atcgcccgct cttcctggac
     2941 cgaatcttcg ggcgtttcgg cgatccggtt cagcacgtcg cgcgcccgcg agtcgtgccc
     3001 gattttgaag aggtaacgcg ggctttccgg catgacgatc atgccggcga tcagcaccag
     3061 cgaagggagt acggcgaacc cca
//
LOCUS       DASUCJ010001009         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1202822, whole genome shotgun sequence.
ACCESSION   DASUCJ010001009 DASUCJ010000000
VERSION     DASUCJ010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202822"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFU90_11970"
     CDS             complement(<1..542)
                     /locus_tag="VFU90_11970"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5480548.1"
                     /translation="MIVVTWNIQWCRGVDGCVDPQRIVREAQALADFDVLCLQEVADN
                     FAHPRLTGASDADQFAAIAGLLPGYTAIEGVAVDRPGDSGRRRRFGNMILSRVPVVQV
                     FRYLLPYPSDPGVPGMPRIAVEAVIEATSGNLRVVTTHLEYYSAVQRAAQVDALRTIY
                     ADGDAHARDFAATIDDGGPF"
     gene            complement(573..>1475)
                     /locus_tag="VFU90_11975"
     CDS             complement(573..>1475)
                     /locus_tag="VFU90_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5480549.1"
                     /translation="LPRAQDALPMSMLPASAGVAASPARELVLYGPGWPVRSLETVAA
                     WLLGILWILPLLYAFWAAFHSSADSTHFTLSAPWTLANFVRAWHAAPFARYFLNTVAL
                     VTMVLAAQLVLVTLAAYAFARFDFPGRGIVFALVLVQLMVMPDVLIVENYRTMTVLGI
                     KDTILSIGLPYMASAFGIFLLRQTFKTVPRELDEAARVEGCSPLATLWKVYVPLARPT
                     YLAYALVSVSYHWNNFLWPLIITNSVESRPVTVGLQVFSATDQGLDWSIISAATLLST
                     GPLLVAFLLFQKQFVQSFMRAGIR"
BASE COUNT          279 a          482 c          503 g          211 t
ORIGIN      
        1 tcgaacggac cgccgtcgtc gatcgtcgcc gcgaagtcgc gggcgtgcgc gtcgccgtcg
       61 gcatagatcg tccgcaatgc atcgacctgg gctgcgcgct gcaccgcgga atagtattcg
      121 agatgcgttg tgacgacgcg caggttcccg gacgtcgcct cgatcaccgc ttcgacggca
      181 atccgcggca tgccgggcac gcccgggtcg ctcggatacg gcagcaggta acggaacacc
      241 tgtaccaccg gcacgcgcga aaggatcatg ttgccgaaac gccgtcgccg tccgctgtct
      301 ccgggccgat cgacggcgac gccttcgatc gccgtgtagc cgggcagcag tccggcgatc
      361 gcggcgaact ggtcggcatc gctcgctccc gtcagtcgcg ggtgcgcgaa gttgtcggca
      421 acctcctgca ggcagagcac gtcgaaatcg gcgagtgctt gcgcctcgcg cacgatccgt
      481 tgcgggtcca cgcatccgtc gacgccacgg caccactgga tgttccacgt gacgacgatc
      541 atcgcgcgtc tgcgctcatc gcgtggcggc gttcaccgga ttcccgctcg catgaacgac
      601 tgcacgaact gcttctggaa gagcaggaac gcgacgagca gcggcccggt cgaaagcagc
      661 gtcgccgcgc tgatgatcga ccagtcgagg ccctggtcgg tggccgagaa cacctgcagc
      721 ccgacggtga ccgggcgcga ctcgaccgag ttggtgatga tgagcggcca caggaagttg
      781 ttccagtggt acgacaccga tacgagcgcg taggcgagat aggtcgggcg cgcgagcggc
      841 acgtagacct tccagagcgt cgcgagcggc gagcagcctt cgacgcgtgc cgcctcgtcg
      901 agctcgcgcg gcaccgtctt gaacgtctgg cgcaggagga agatgccaaa ggcgctcgcc
      961 atgtaaggca ggccgatcga gaggatcgtg tccttgatgc cgagtaccgt catcgtccgg
     1021 tagttctcga cgatcagcac gtcgggcatg accatcaact gcaccagtac gagcgcgaac
     1081 acgatcccgc gcccgggaaa atcgaaacgc gcgaacgcgt acgcggcgag cgtcaccagg
     1141 acgagctggg cggcgagcac catcgtgacc agcgcgacgg tgttgaggaa gtagcgcgcg
     1201 aacggcgccg cgtgccacgc gcgcacgaag ttcgcgagcg tccacggcgc cgacagcgtg
     1261 aagtgcgtcg agtcggcaga agaatggaac gcggcccaga acgcgtagag aagcggcagg
     1321 atccagagga tcccgagcag ccacgctgcg acggtttcga gcgagcgcac cggccagccg
     1381 ggcccgtaca gcaccagctc ccgcgccggt gacgccgcaa cccccgcgga agccggcagc
     1441 atgctcattg gtaatgcgtc ctgcgctcga ggaag
//
LOCUS       DASUCJ010001010         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_499243, whole genome shotgun sequence.
ACCESSION   DASUCJ010001010 DASUCJ010000000
VERSION     DASUCJ010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499243"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2191)
                     /gene="ileS"
                     /locus_tag="VFU90_11980"
     CDS             complement(<1..>2191)
                     /gene="ileS"
                     /locus_tag="VFU90_11980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5480550.1"
                     /translation="RRLERNAPAEPWILHDGPPYANNDLHMGTFLNMVLKDVFVKIAL
                     LDGKYAKFVPGWDMHGLPIELETLKHLGITDFHAIDPLELRAKCRERALFWLDRQRET
                     RVRMGNFGLWDRPYRTIDPSFEATIVTTLAELAEKQQIYKGLCSTLWCVHDETALAEA
                     EIEYEQKVSPSIYVRFPANAAQRRELLAAFALPEGADEKPLSLLVWTTTPWTLPANVA
                     IALRPDATYGLYDLGDERVVVAVDLAARTLGERFAGATLLAQGTGREFDGLAVRHPLF
                     DRDSQLVLADYVDLETGTGLVHTAPGHGADDFDTGVKYNLPILNPVDAAGRFTSEAGP
                     YAGLNIFAANPKIVDDLRAAGALWSAQEYEHSYPHCWRCHNPVIFRATAQWFIAMDQN
                     VLRQRTIDAIDAVEYVPGWGQLRQRQMIETHPEWCISRQRTWGTPIPALVCRECGQSV
                     LDPGVARLAAKRFGEAGSDAWWTDPVESFLPPHFACPNCRGTSFDKEKNIVDIWFESG
                     VTHLAVLGRDGLTWPSDAVLEGGDQYRGWFRSSLITGVAIKGRAPYRSVIKNGWVNDE
                     QGRAMSKSSGTGIAAREAMEKWGADVLRLWVASVEFVDDVRFGPNVVEQTGRVYRNLR
                     NRIRFMLSNLDDLDPADVLAPEHMQPLDRLACAVTDAFAAEVRRAYRHYAIHDAYLRV
                     VEFESAMSSFYFDALKDPLYSRALADPRRRSAQSALLYVLTRFLTALA"
BASE COUNT          392 a          734 c          690 g          375 t
ORIGIN      
        1 tgccagcgcc gtaaggaaac gcgtcagcac gtacaacagc gccgactgcg cgctgcgccg
       61 gcgcggatcc gcgagcgccc gcgaatacag cggatccttc agcgcgtcga aataaaagct
      121 cgacatcgcg ctttcgaatt ccacgacgcg gagataggcg tcgtggatgg cgtagtgccg
      181 gtacgcgcgg cgcacttcgg ccgcgaacgc gtcggtgacg gcgcacgcca gccggtcgag
      241 cggctgcatg tgctcgggag cgagcacgtc ggccggatcg aggtcgtcga ggttggaaag
      301 catgaaccgg atgcggttgc gcaggttccg gtacacccgg ccggtctgtt cgacgacgtt
      361 gggcccgaag cgaacgtcgt cgacgaactc taccgacgcg acccacaggc gcagcacgtc
      421 cgcgccccac ttctccatcg cctcgcgcgc tgcgatgccg gtaccggacg acttcgacat
      481 cgcccgtccc tgctcgtcgt tgacccaccc gttcttgatg acgctgcggt acggcgcgcg
      541 tcctttgatc gcaacgccgg tgatcagcga gctgcgaaac cagccgcgat attgatcgcc
      601 gccttcgagc accgcgtcgc tcggccaggt gagtccgtcg cgtcccagca ccgccaaatg
      661 cgtgacgccc gattcgaacc aaatatcgac gatgtttttc tctttatcga acgacgtgcc
      721 gcggcagttc gggcaggcaa aatgcggtgg aagaaacgat tcgaccggat cggtccacca
      781 cgcatccgag ccggcttcgc cgaagcgctt ggcggccaga cgcgcgaccc cgggatcgag
      841 gacggactgg ccgcactcgc ggcacaccag cgccggaatc ggcgtccccc aggtgcgctg
      901 ccgcgagatg caccattcgg gatgcgtttc gatcatctgg cgctgccgca gctgtcccca
      961 gccgggcacg tactcgaccg cgtcgatcgc gtcgatcgtt cgctggcgca gcacgttctg
     1021 atccatcgcg atgaaccact gggcggttgc ccggaaaatc acggggttat gacagcgcca
     1081 gcagtgcgga tacgagtgtt cgtactcctg cgcgctccac agcgctccgg cggcccgtaa
     1141 gtcgtcgacg atcttcggat tcgcggcgaa aatgttcagg ccggcgtagg gaccggcttc
     1201 gctcgtaaac cgcccggccg cgtcgaccgg attgagaatc ggcaagttgt acttcacgcc
     1261 ggtatcgaaa tcgtcggcac cgtggcccgg cgcggtgtgc accaagccgg tgccggtttc
     1321 cagatcgacg taatccgcaa ggaccagctg cgagtcgcga tcgaagagcg gatggcgtac
     1381 cgcgagcccg tcgaactctc tacccgttcc ctgcgcgagc agcgtggcgc cggcaaagcg
     1441 ctcgcccagc gtccgcgcgg ccaagtccac cgccacgacg acgcgttcgt cgcccaagtc
     1501 gtacagaccg tacgtcgcgt ccgggcgcag cgcgatcgca acgttcgccg gcagcgtcca
     1561 cggcgtcgtc gtccagacga gcagcgacaa cggcttctcg tccgctccct ccggaagtgc
     1621 gaacgcagcg agcagctcgc ggcgctgcgc cgcattggcg ggaaagcgga cgtagatcga
     1681 cggcgatact ttttgctcgt attcgatctc cgcttcggct aacgccgttt cgtcgtgaac
     1741 gcaccacagc gtcgaacaca gccccttgta gatctgctgt ttctccgcca gctcggccag
     1801 ggtcgtaacg atcgtcgcct caaacgacgg atcgatcgtg cggtaggggc gatcccacaa
     1861 accgaaattg cccatccgca cgcgcgtttc gcgctggcgg tcgagccaga aaagggcgcg
     1921 ttcgcgacat ttggcgcgca gctcgagcgg atcgatcgca tggaaatcgg tgatccccaa
     1981 gtgcttgagc gtttccagct cgatcggcaa gccgtgcatg tcccagccgg gcacgaactt
     2041 cgcatacttc ccgtcgagga gcgcgatctt cacgaacacg tccttgagaa ccatgttcag
     2101 gaacgtcccc atgtgcaagt cgttgttggc atagggcggc ccgtcgtgca ggatccacgg
     2161 ctcggccggc gcgttgcgct ccagccggcg t
//
LOCUS       DASUCJ010001011         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_407598, whole genome shotgun sequence.
ACCESSION   DASUCJ010001011 DASUCJ010000000
VERSION     DASUCJ010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407598"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..>1085
                     /locus_tag="VFU90_11985"
     CDS             262..>1085
                     /locus_tag="VFU90_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480551.1"
                     /translation="MRATWRDSLMEAMLRAALGALAGAAVGAAIAPLTRGLFHVPGGG
                     VGWITVHHYPKGFDYAVIALLMIGSAAGAWIASRNGAPASAGRIPAKAGAPLGVLAAV
                     VFLLMLFIHDHPYSLMDPFHEGEYLTPAYLLMEGGRPYADVFFFHGFATDGGLDSLIL
                     DHSPKRERRLETILDAATLALLVPIAVEICATTGGVIAGVLAALSAIGAFEVPVFPYF
                     RLAPILISTLALLRFARTRRTGWIITAYCASTLGLLWSVDVGVYAVAATLILAIWLR"
BASE COUNT          159 a          347 c          351 g          228 t
ORIGIN      
        1 atcggatacc agccgatcga tggcgacgtg atcgcccagc cgatcgactc gccggtcagg
       61 cccgatgtca cgtgcgtgtt ttcgtagatg aactggccgt cgtcgtagtt gagaaactga
      121 tgcgtccgca cctgggcgaa cacaatcagc gtgatcacgg cgagtgcggc gaggaaagga
      181 acggtcccga gtcccaagtc ccaagtggcg ggtcgctgac gcgggacgcg ggacacggga
      241 cccgaaacct ttgttttcgt cttgcgcgcc acgtggcgcg acagtttaat ggaggcgatg
      301 cttcgcgccg ccctcggtgc gcttgccggt gcggcggtcg gcgccgcgat cgctccgctg
      361 acccgcggcc tgttccatgt gcccggcggc ggggtgggct ggatcaccgt tcaccactat
      421 ccgaaaggct tcgattacgc ggtcatcgcg ctgttgatga tcggatcggc ggcgggagcg
      481 tggatcgcgt cgcgaaatgg agcaccggct tcagccggtc gcatcccggc taaagccggg
      541 gctccattgg gcgttctcgc agctgtcgtc tttctcctga tgctctttat ccacgaccat
      601 ccgtactcgc tcatggatcc gtttcacgaa ggcgagtacc tcacgccggc gtatctattg
      661 atggaaggtg gccggccgta tgcggacgtt ttcttctttc acggcttcgc gaccgacggc
      721 ggcctcgact cgctgatcct cgaccactcg ccgaaacgcg agcggcggct ggaaacgatt
      781 ctcgacgcgg cgactctcgc gctgctcgtg ccgattgccg tggagatctg cgccaccacc
      841 ggcggagtca tcgccggcgt gctcgcggcc ctcagcgcga tcggggcgtt cgaggtgcct
      901 gtctttcctt actttcgcct cgcgccgatt ctgatctcga cgctcgcgct gttgcgcttc
      961 gcgcgaacgc gacgcactgg ctggatcatc accgcgtatt gcgcctcgac cctcggcctg
     1021 ctctggagcg ttgatgtggg cgtgtatgcg gtcgcggcga cactcatcct ggcgatctgg
     1081 cttcg
//
LOCUS       DASUCJ010001012         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1263988, whole genome shotgun sequence.
ACCESSION   DASUCJ010001012 DASUCJ010000000
VERSION     DASUCJ010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263988"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFU90_11990"
     CDS             <1..801
                     /locus_tag="VFU90_11990"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU5480552.1"
                     /translation="LAAFPAAFSIIIPALYFPILLMLLGLMFRGVAFEFRSRDPGHQR
                     WWNRAFFGGSVVATFSQGIVLGMFVQGFTVTGRAFTGTSFDWLASFPLVTGAGLVAGY
                     VLLGSAWLVMKTEGALQTWARRWATLALVAMLAFIVVVSVWTPLLHEEIAARWFTWPN
                     LAWFAIVPLLTALIAWATWRSLRSSSDYVPFVGAMGLFAMSYAGLGISVFPYIVPYRL
                     TLWDAAAAPSAQAFLVIGTLFLLPIIFMYLGWAYYVFRGKVSADVGYH"
     gene            complement(804..>2031)
                     /locus_tag="VFU90_11995"
     CDS             complement(804..>2031)
                     /locus_tag="VFU90_11995"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU5480553.1"
                     /translation="VRTRCSDDYLWLPFATCRYVAATGDTGVLDEPAPFLDGRALRDA
                     ETSYYDLPGQSGQVASLYDHCVRAIEHGLRFGAHGIPLMGSGDWNDGMNLVGAGGKGE
                     SVWLGFFLYAVLTQFGELAQRHGDATLAERCSSEAARLRDAIEAHCWDGAWYRRAWFD
                     DGSPLGTSSNTECRIDSVAQSWSVLSGAGRVEHARMAMDAVDRQLVHREAALVQLLDP
                     PFDRSEPNPGYIQGYVRGVRENGGQYTHAAVWAAMAFAALGDRRHAWELFAMLDPVNH
                     AKSSDAIAIYKTEPYVLASDVYALAPHRGRGGWTWYTGSAGWMYRLILESLLGLRRDA
                     ERLHFSPCVPANWTSFGVRYRYRETLYRITVMPTHGSDDASTLTLDGVMLPDLAISLT
                     DDRRPHSVAVSVRAAR"
BASE COUNT          311 a          701 c          635 g          384 t
ORIGIN      
        1 ctggccgcgt tcccggccgc gttctcgatc atcatcccgg cgctctactt tccgattctc
       61 ctgatgctgc tcgggctcat gtttcgcggt gtcgcgttcg agttccgctc gcgcgatccc
      121 ggacaccagc gctggtggaa ccgcgcattc ttcggcgggt cggttgtcgc gacgttctcg
      181 cagggcatcg tgctcgggat gttcgtgcag ggtttcacgg tcaccggtcg cgcattcacc
      241 ggcacgagct tcgactggct ggcgtcgttc ccgctcgtca ccggcgcggg cctcgtcgcc
      301 ggctacgtcc ttctcggctc ggcgtggctc gtgatgaaga ccgaaggcgc gcttcagacc
      361 tgggcgcgcc gctgggcgac gcttgcgctc gtcgcgatgc tcgccttcat cgtcgtcgtc
      421 agcgtctgga cgccgctcct gcacgaagag atcgcggcac gctggttcac gtggcccaac
      481 ctcgcgtggt tcgcgatcgt gccactgctg acggcgctga tcgcgtgggc gacctggcgc
      541 tcgctgcgca gcagcagcga ctacgttccg ttcgtcggcg cgatgggtct cttcgcgatg
      601 agctacgcgg ggctcggcat cagcgtgttt ccttacatcg tcccctatcg actcacgcta
      661 tgggacgcgg ccgcagcgcc ctccgcgcag gcgttcctcg tgatcggcac gctgttcctg
      721 ctgccgatca tcttcatgta tctcggctgg gcgtattacg tgttccgcgg gaaggtttcg
      781 gcggacgtcg gatatcactg acttcaccgt gccgcgcgca cgctcaccgc gaccgagtgc
      841 ggccgccggt catcggtcaa ggaaatcgcc aggtcaggca gcatgacacc gtcgagcgtc
      901 aacgtcgacg catcgtccga accgtgcgtt ggcatcaccg tgatccggta gagcgtctcc
      961 cgatagcgat atcgcacgcc gaaggaagtc caattcgccg gcacgcacgg agaaaaatgg
     1021 agcctctccg cgtcgcgtct caatcccagc agcgattcca gtatcagccg gtacatccag
     1081 ccggccgagc cggtgtacca ggtccagccg ccgcgccccc tgtgcggcgc aagtgcatag
     1141 acgtctgacg cgagcacgta aggctccgtc ttatagattg cgatcgcatc cgacgatttt
     1201 gcgtgattga ccggatcgag catcgcaaac agttcccacg cgtgcctgcg gtcgccgagg
     1261 gccgcgaatg ccatcgccgc ccacaccgcc gcgtgcgtgt actgaccacc gttctcccgc
     1321 acaccgcgta cgtatccttg tatgtatccc ggattcggct cggagcgatc gaacggtgga
     1381 tccagcaatt ggacgagcgc agcctcgcgg tgaacgagct gtcgatcgac cgcatccatc
     1441 gccatgcggg cgtgttccac ccgcccggcc cccgacaaga ccgaccagct ctgcgcaacc
     1501 gaatcgatcc ggcattcagt gttgctcgac gttccgagcg gcgagccgtc gtcgaaccag
     1561 gcgcggcggt accaggcgcc gtcccagcaa tgcgcttcga tggcgtcgcg caatcgggcc
     1621 gcttcgctcg aacagcgctc ggcgagcgtc gcgtcgccgt gtcgctgggc cagttcgccg
     1681 aattgggtca gcacggcgta caggaagaac ccgagccaga cgctctcccc tttgccgccg
     1741 gcgcccacca ggttcatgcc gtcgttccag tcgcccgagc ccatcagcgg tatgccgtgc
     1801 gccccgaacc gcagcccgtg ctcgatcgcg cgcacgcagt gatcgtaaag gctcgcgacc
     1861 tggccggatt gcccgggtaa atcgtagtac gacgtctcgg cgtccctgag cgcgcggccg
     1921 tccaggaatg gcgccggttc gtcgagcacg ccggtatcgc cggtcgctgc aacgtagcgg
     1981 caggtcgcga acggcagcca tagatagtcg tccgaacagc gggtgcgtac c
//
LOCUS       DASUCJ010001013         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_300968, whole genome shotgun sequence.
ACCESSION   DASUCJ010001013 DASUCJ010000000
VERSION     DASUCJ010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300968"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFU90_12000"
     CDS             complement(<1..734)
                     /locus_tag="VFU90_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480554.1"
                     /translation="MATVRYEHVTKRYGDVFAVNDLSIEIADKEFLVLVGPSGCGKST
                     VIRLLAGLEPATAGSIYIDGEAVDDVPARDRDIAMVFQSYALYPHMSVYDNMAFGLKM
                     HGMKHDEIRRRVHEAAATLQIDELLKRKPGQLSGGQRQRVALGRAIVREPRVFLFDEP
                     LSNLDAKLRVQTRAELSKLHARLQTTFIYVTHDQIEAMTMASRIAVIKDGILQQVGTP
                     EELYFRPDNVFVGGFIGSPSMNFLPAR"
     gene            complement(747..>1012)
                     /locus_tag="VFU90_12005"
     CDS             complement(747..>1012)
                     /locus_tag="VFU90_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480555.1"
                     /translation="ISMPAFVVAAIWQFTAIWNDFLFAVTVTGNPGTQPATVALNNLA
                     GSFIVEWNVQMAGALIAALPTLIVYIVLGRFFVRGLMAGALRS"
BASE COUNT          175 a          320 c          335 g          182 t
ORIGIN      
        1 cgcgccggaa ggaagttcat cgatggactc ccgatgaatc cgccgacgaa cacgttgtcc
       61 gggcggaaat acagctcttc gggcgtcccg acctgctgca ggatcccgtc cttgatcacc
      121 gcgatccggc tcgccatggt catcgcctcg atttgatcgt gagtgacgta gatgaacgtt
      181 gtctgcagcc gggcgtgcag cttgctcagc tccgcgcggg tctggacgcg aagcttcgca
      241 tcgaggttcg atagcggctc gtcgaacagg aagacgcgag gttcgcggac gatggcacgg
      301 cccagcgcga cgcgctggcg ctgacccccg gagagctgac ccggcttgcg cttcagcagt
      361 tcatcgatct ggagcgtcgc ggcggcctcg tggacccgac ggcggatctc gtcgtgcttc
      421 atgccgtgca tcttgagtcc gaacgccatg ttgtcgtaga cggacatatg cggatacagc
      481 gcgtacgact ggaagaccat ggctatgtcg cggtcgcgcg cgggcacatc gtcgacggcc
      541 tcgccgtcga tgtagatgct tccggcggtc gccggctcga gcccggcgag caggcggatc
      601 acggtggact tcccgcaccc cgatggtccc acgagcacca ggaactcttt gtccgcgatc
      661 tcgatgctga gatcgtttac cgcgaacacg tcgccgtagc gtttcgtgac gtgctcgtag
      721 cggaccgtgg ccatgcgttc cctcccctag ctgcggagcg cgccggccat gagcccgcgc
      781 acgaagaacc ggccgagcac gatatacacg atgagcgtcg gcagcgcggc gatcagcgcg
      841 ccggccatct gcacgttcca ttcgacgatg aagctgccgg cgagattgtt gagcgcgact
      901 gtcgccggct gcgtccctgg attgccggtg acggtgaccg cgaagaggaa gtcgttccag
      961 atcgcggtga actgccagat ggcggcgacg acgaacgccg gcatcgagat cg
//
LOCUS       DASUCJ010001014         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1370925, whole genome shotgun sequence.
ACCESSION   DASUCJ010001014 DASUCJ010000000
VERSION     DASUCJ010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370925"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFU90_12010"
     CDS             complement(<1..258)
                     /locus_tag="VFU90_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480556.1"
                     /translation="MRVTLIQPPQGSRFGFSKVLRVEPLGLECVGAAIRSREHEVVLI
                     DRRLEDQSKLVDHLRSSRPAGVGISCGFTSDVYTTLATARLV"
     gene            complement(457..1005)
                     /locus_tag="VFU90_12015"
     CDS             complement(457..1005)
                     /locus_tag="VFU90_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480557.1"
                     /translation="MRSRPALASYVAPPIALAARVALVALASCSAPAAMSTVGVSVPR
                     APEATAEVDAGDAAPPPPPPVAADFRSHMNRLSDRFLSRGHAERFDAVIWGNAQVAPD
                     ASAAQDFPEGAVLVEEAVVRDTRGDRPFGLLVMTKEKDSFRFDAVDPDGRVAPPDRVA
                     ACVTCHQEAAHFVFPWRRPEGP"
     gene            complement(1020..>1333)
                     /locus_tag="VFU90_12020"
     CDS             complement(1020..>1333)
                     /locus_tag="VFU90_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480558.1"
                     /translation="CGSLGSNVPGNQNAHMPYDGSTICANKLSNGAPNNGDYNNDRTF
                     SQARIHRWRGLVGIDYRYELIDLAAEFATDVTDPSDENANLGISGDRQWTISLKTGVS
                     F"
BASE COUNT          217 a          411 c          486 g          219 t
ORIGIN      
        1 aacgagcctg gcggtggcga gggtcgtgta cacgtccgac gtgaagccgc agctgatgcc
       61 gacccctgcg ggtcgcgacg atcgcagatg atcgaccagc ttcgattggt cttcgagccg
      121 gcgatcgatg agcacgactt cgtgttcgcg gctccggatg gccgcgccga cgcactcgag
      181 gccgagcggc tcgacgcgca agactttgct gaatccgaag cgagatccct ggggcggttg
      241 gatgagcgtg actcgcatgg gcatggctcc gcgaaagacg gcgcgcctcc ttgggccgca
      301 cgatggcgta ccggcgcgcg ttgacgagtt cgttcgtgga gtgcacccgt ccgtcgaggc
      361 tcggggcact tctcccaaac gtcccccatg aaacgcaaaa gtcgaggggc gattgcagtc
      421 gagaggcgat tgcagattgg tagagcggag ttcgctctac gggccctcgg gccgtctcca
      481 cggaaacacg aaatgagccg cctcttggtg gcacgtgacg catgcggcga cgcgatccgg
      541 aggcgcgacg cggccgtccg ggtcgacggc gtcgaagcga aacgagtctt tctctttggt
      601 cattacgagc aggccgaacg ggcggtcgcc ccgggtatcg cggacgaccg cttcttcgac
      661 gaggacggcg ccttcgggga agtcctgggc ggcgctcgcg tcgggcgcga cctgcgcgtt
      721 gccccagatg acggcgtcga agcgttcggc gtggcctcgc gagaggaacc gatccgacag
      781 gcgattcatg tgagaacgaa agtcagcggc gacgggcgga ggcggcgggg gcgccgcgtc
      841 gccggcgtcg acctcggcgg tggcctccgg ggctcgcggt accgacacgc cgacggtcga
      901 catcgccgcg ggggcgctgc acgaggcgag cgcgacgagc gcaacccgcg cggcgagcgc
      961 gatcggcggc gcaacatacg aggcgagcgc gggtcgcgag cgcacggggt gggtcgcgat
     1021 cagaacgaga cgccggtctt gagggaaatc gtccattgcc gatccccgga gatacccaga
     1081 tttgcgtttt cgtcgctagg gtccgtcaca tccgtcgcga actcggccgc cagatcgatg
     1141 agctcgtagc ggtaatcgat cccgacgagc ccgcgccagc ggtggatccg tgcctggctg
     1201 aacgtgcggt cgttgttgta gtccccgttg ttcggagcgc cgttcgagag cttgttcgcg
     1261 cagatcgtcg acccgtcgta cggcatatgg gcgttctggt tgcccggtac gttgctgccg
     1321 agcgagccgc act
//
LOCUS       DASUCJ010001015         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1172263, whole genome shotgun sequence.
ACCESSION   DASUCJ010001015 DASUCJ010000000
VERSION     DASUCJ010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172263"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFU90_12025"
     CDS             <1..266
                     /locus_tag="VFU90_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5480559.1"
                     /translation="PGLHGYAIMTAIQERSGDVLRAEEGSLYPALHRMEEAGWMRARW
                     ITKDTGRRARIYDITTAGRSQLAAEEARWRAVTAAVNRVLRTV"
     gene            268..>1046
                     /locus_tag="VFU90_12030"
     CDS             268..>1046
                     /locus_tag="VFU90_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480560.1"
                     /translation="MAWWSRLARTFRPTRVTDDIDEELASHLEEAIARGRDPEEARRA
                     LGVPLRHREASRDIRVLPWLDALRADVRFGGRQILKNRVTSAAAILSLGLAMGACVAA
                     FRIVDAVLLRPLPYADPNGLVMLWEHHMSDVGASHNVTGPANFLDWRDEARSFSAMAA
                     FASARVTIVAPGGDPVSEQARYANAALLPMLGVKPVLGRLYGEADDQAGAARVAVLDY
                     GLWQQHFGGRRDIVGRTVQLNGNDVTVVGVLPKGFAFFEPAA"
BASE COUNT          156 a          333 c          377 g          180 t
ORIGIN      
        1 gtccgggctt gcacggctac gcgattatga cggctatcca ggagcggtcc ggcgacgtcc
       61 tccgcgccga agagggttcg ctctaccctg ccctccatcg gatggaagag gccggctgga
      121 tgcgggcgcg atggatcacg aaagacaccg ggcgccgagc tcgcatctac gacatcacga
      181 ccgccggacg atcgcagctc gccgccgaag aggcgcgttg gcgcgccgtc acggcggccg
      241 tcaatcgcgt cctgaggacg gtgtgacatg gcgtggtggt cgcgactcgc gcgcacgttc
      301 cggcctactc gcgtcaccga cgacatcgac gaagagctgg cgtcacacct cgaggaggcg
      361 attgcgcgtg gccgcgatcc agaggaggcg cgccgcgcgc tgggcgtgcc tctgcgccat
      421 cgcgaagcga gccgcgacat ccgtgtgctg ccctggctcg atgcgcttcg tgcggacgtc
      481 cgattcggcg gccgtcaaat cctgaagaac cgcgttacgt cggcggccgc gattctctcg
      541 ctgggtctgg cgatgggcgc ctgcgtcgcc gcgttccgca tcgtcgacgc cgtcttgctg
      601 cgaccgctgc cgtacgccga tccgaacggt ctcgtgatgt tgtgggagca tcacatgtcg
      661 gatgtgggtg cgtcgcacaa cgtgacgggt ccggcgaact tcctcgattg gcgtgacgag
      721 gcacgatcat tttccgcgat ggcggcattt gccagcgcgc gggtcacgat cgtggcgccg
      781 ggcggagatc cggtgtcgga gcaagcgcgc tacgcgaacg cggcgctgct gccaatgtta
      841 ggcgtgaagc cggtgctcgg ccgattgtac ggcgaggcgg atgatcaagc gggtgccgcg
      901 cgcgtggctg tgctcgacta cggactctgg cagcagcact tcggcggcag gcgcgacatc
      961 gttggccgca cggtgcagct caacggcaac gatgtcaccg tggtgggcgt gctgcccaag
     1021 gggttcgcgt tcttcgagcc ggcggc
//
LOCUS       DASUCJ010001016         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_193998, whole genome shotgun sequence.
ACCESSION   DASUCJ010001016 DASUCJ010000000
VERSION     DASUCJ010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193998"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFU90_12035"
     CDS             complement(<1..321)
                     /locus_tag="VFU90_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5480561.1"
                     /translation="MRPHLGVTSAAVFIVTLAAVACLDTSSPNLDLPNEVVAIRMQPT
                     SASVPLGGHVSFQAVAVDNSGDPVSGQSVTWSSQDTTVAVVDANGNVSTKKIGSTQIV
                     ASGDS"
     regulatory      complement(347..439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            789..>1723
                     /locus_tag="VFU90_12040"
     CDS             789..>1723
                     /locus_tag="VFU90_12040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5480562.1"
                     /translation="MGEALAARLASVGWTTLTTSDKRARAARLADMVSTVWRRRADYD
                     VAVVDVYSGAAFVWAEAVTATLRAARRPYVITLHGGNLPAFAARWPRRVRRLLHRAHT
                     VTTPSMYLRDRLRFVRDDLALVPNAIELGSYRFRERTRAVPRLVWLRAFHRVYNPTLA
                     VRAIARLTQAMPNVHLTMVGSDTGDGTHAATREAIAQAGLGAHVELRGGVPKADVPNV
                     LGEADVFLNTSTIDNTPVSVIEAMACGLCVVSTSVGGIPFLVDDTHDGLLVPSDDADA
                     MARAVKRIVGEPELAAGLSRGARAKVEAFDWSAVLP"
BASE COUNT          274 a          512 c          609 g          328 t
ORIGIN      
        1 cgagtcgccc gacgcgacga tctgcgtcga accaatcttt tttgtggaga cgttgccgtt
       61 cgcatcgacg acggcgacgg tcgtgtcttg gctggaccag gtgacggatt gccccgagac
      121 gggatcgccg gagttgtcga cggcgacggc ttggaacgac acgtggccgc ccaagggcac
      181 gctcgcggac gtgggctgca tcctgatggc cacgacctcg ttggggaggt cgaggttggg
      241 cgaggatgtg tcgagacagg cgacagcggc gagggtgacg atgaacacgg ccgctgatgt
      301 tacgccgaga tggggacgca tgccaaaccc ctccgtgaag gatccgttcg gcggcccgac
      361 tggcgtgacg cgtctcaaga cgcgcggtag cctcgtagct ttgcgtcgcc ggctttcgcc
      421 gggtttgccg ttgtcgcggc ccctcggtga ggggtcgtgc gatcgtgcct cgtttcgatt
      481 gtgttttccg agttactctc cggagacgcg cgttctgtgg tcgcgtaacc ataaggaaat
      541 tcacatactt agagcgtggt cacggcgaga gaaaatgtta cggcggccac gaattgttgc
      601 atggacagga aatgatgatc ggagttgcgt gataagacgc gttgcggatg cgatcgtctt
      661 tgcggtgcaa tgatgaagtc ggctctggca attcatggtg agtcatgaca tcgtgcggtc
      721 ccgcaccgat gactccacgc gttctcttcg tcggaaattt tctgtcgagc tcgagagcga
      781 gcctgacagt gggcgaagca ttggccgcgc gactcgcttc ggtggggtgg acgaccctca
      841 cgacctcgga caagcgcgcg cgtgccgcgc gacttgccga catggtgagc acggtctggc
      901 ggcgccgcgc cgactatgac gtggccgtgg tggacgtcta ctcgggcgcc gcgttcgtgt
      961 gggccgaggc cgtgacggcg acactgcgcg cggcccgtcg accgtacgtg atcacgctcc
     1021 acggcggcaa cctgcccgcg tttgcggcgc ggtggccgcg acgggttcgg cgcctactgc
     1081 atcgcgcgca cacggtcacg acgccatcca tgtatctccg cgaccgtttg cgattcgtgc
     1141 gcgacgacct cgccctcgta ccgaacgcga tcgagctcgg gtcctatcgc tttcgggagc
     1201 ggacgcgcgc ggtgccgcgc ctcgtgtggc tgcgcgcctt tcatcgcgtc tacaatccga
     1261 cgctggccgt gcgggcgatt gcacgcctaa cgcaagcgat gccgaatgtc catctgacga
     1321 tggtgggttc ggataccggc gatggcacgc acgccgcgac ccgagaggcc atcgcgcaag
     1381 ccggactcgg cgcgcacgtg gagctgcgcg gcggtgtgcc caaggctgac gtgccgaacg
     1441 tgctcggcga agcggacgtg tttctcaata ccagcaccat cgacaacacg cccgtgagcg
     1501 tgatcgaggc gatggcgtgt ggtttgtgcg tcgtgagcac gtctgtgggc ggcattccgt
     1561 ttctcgtcga cgacacacac gacggtttgc tcgtgccgag cgatgacgcg gacgcgatgg
     1621 ctcgagcggt gaagcgcatc gtcggcgagc cggagctggc ggcggggttg tcgcgcggcg
     1681 cgcgcgccaa ggtggaagcg ttcgactgga gcgcggtctt gcc
//
LOCUS       DASUCJ010001017         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1370977, whole genome shotgun sequence.
ACCESSION   DASUCJ010001017 DASUCJ010000000
VERSION     DASUCJ010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370977"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFU90_12045"
     CDS             <1..1353
                     /locus_tag="VFU90_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5480563.1"
                     /translation="VDREYGGMRRGEMWVVNDPYVAGTHLNDVTVVRPVFFNGMLAGF
                     AANKAHHTDVGGSVPGSMPADAPDLFAEGLVVPPMRLVDGDEPVAATVALFRANSRTP
                     DARSGDLRAQTAGNYTGERRLLELYERYGAGIVEAAVASLLDASERRMRNALRALRNG
                     VFESADFLEGLDGAASIRIALKLTLNDGTARFDYAGTSEQVGFPLNAVFGVTLSGIHY
                     ALRAATDPTIPMNDGCFRPVEAIVPEGTLLNPRRPAPVSGGNVETSTRNADVVLRALA
                     GGAPERLPASSGGTMSNVMLGGTRPDGRAWTFYETNGCGMGARPTSDGLDGIQCHMTN
                     TLNTPIEAIETEFPLRVVRYEFAPGTGGAGRYRGGDGLIRAIALTEGSAQASLLADRH
                     VLPPPGAQGGQPGAVGRHALLRNGVETPLPAKTHLTLQPGDVLIVQTPGGGGYGTPNG
                     "
     gene            complement(1359..>2153)
                     /locus_tag="VFU90_12050"
     CDS             complement(1359..>2153)
                     /locus_tag="VFU90_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480564.1"
                     /translation="TVRGRPGWHRHLDRRFRNRASLEWFTAAGEAAPRVTLERSGATA
                     VMRVRGAADFAIPWLPCAAFERVAELPLRRATAACATVALAGFESLPDDRKRELVAAL
                     EALAGREPALRLLVVGEESVVESLRARFGDRTSRLDLEAGGKPSAAGAACVLHAGATD
                     ESAFGNVLLAQCDLPALVLEGTEPAALFAGDVVVTAAPGEIADRLAPLFSDAQARERY
                     GTQSAADARRRFSPRRTAIRVVDLLCAARFGFERPAQARSNAPIPL"
BASE COUNT          358 a          771 c          746 g          278 t
ORIGIN      
        1 gtcgatcgcg agtacggcgg catgcgccgc ggtgagatgt gggtggtcaa cgatccgtac
       61 gttgcgggga cgcacctgaa cgacgttacg gtcgtgcgac cggttttctt caacggaatg
      121 ctggccggtt tcgccgccaa caaggcccac cacaccgacg tcggcggcag cgttccgggc
      181 tcgatgccgg ccgacgcccc cgacttgttt gccgaagggc tggtcgttcc gccgatgcgg
      241 ctggtcgacg gcgacgagcc ggtcgcggcg acggtcgcgc tcttccgcgc caactcccgc
      301 acgcccgacg cccgcagcgg ggatctgcgc gcgcaaaccg ccggcaacta cacgggcgag
      361 cgccggctgc tggagcttta cgagcgatac ggcgccggca tagtcgaagc cgcggtcgcg
      421 agcctcctcg atgccagcga acggcgcatg cgtaacgcgc tgcgcgccct ccgcaacggc
      481 gtcttcgaaa gcgccgactt cttggaaggc ctcgacggcg ctgcgtcgat tcgcatcgcg
      541 ctcaagctga cgctgaacga cggaacggca cgattcgatt acgcgggaac ctcggaacag
      601 gtcggctttc cgctcaacgc cgtattcgga gtaacgctgt cgggaatcca ctacgcgctg
      661 cgggccgcga cggatcccac gattccgatg aacgacggct gcttccgtcc ggtcgaagcg
      721 atcgttcccg aaggaacgct gctcaacccg cgccggcccg cgccggtttc cggcggaaac
      781 gtcgagacga gcacccgcaa cgccgacgtc gtgctgcgtg cgctcgccgg gggagcgccg
      841 gaacgtctgc ccgcgtcgag cggcggaacc atgagcaacg tcatgctcgg agggacgcgg
      901 cccgacggcc gcgcgtggac cttctacgaa accaacggct gcggaatggg cgcgcgcccg
      961 accagcgacg gcctcgacgg catccaatgc cacatgacca acacgttgaa cacgccgatc
     1021 gaagcgatcg aaacggagtt cccgctgcgc gtcgtgcgct acgagttcgc gcccggcacc
     1081 ggcggcgccg gccgttaccg cggcggcgac ggattgattc gcgcgatcgc gctcaccgaa
     1141 ggcagcgcgc aagcgtcgct gctcgcggat cgccacgtgc tcccgccgcc cggcgcgcaa
     1201 ggcggccaac cgggagcggt cgggcggcac gcgctgcttc gtaacggcgt cgaaacgccg
     1261 ctgcccgcta agacgcatct cacgctccaa ccgggcgacg tgctgatcgt ccaaacgccg
     1321 ggcggcggcg gatacggaac gccgaacggc tgacgccgct acagcgggat cggagcgttg
     1381 ctgcgcgctt gcgccgggcg ttcgaagccg aaccgcgccg cgcacagcag gtcgacgacg
     1441 cgaatcgcgg ttcggcgcgg tgaaaaccgg cggcgcgcgt cggctgccga ctgcgtgccg
     1501 taccgctcgc gcgcctgcgc atccgaaaag agcggcgcga gacgatccgc gatttcgccg
     1561 ggcgcagccg tcacgacgac gtcgccggcg aacaacgccg ccggttcggt gccttcgagg
     1621 accagcgccg gcaagtcgca ctgcgcgagc aacacgttgc cgaacgcgct ttcgtcggtc
     1681 gcgccggcgt gcagcacgca cgcggcgccg gccgcggacg gtttcccgcc ggcctcaagg
     1741 tcgagccggg aagtacgatc gccgaagcgc gcccgcagcg attccacgac cgattcttcg
     1801 cccacgacga gcagacgcag cgcgggctcg cggccggcaa gcgcctcgag cgccgccacc
     1861 agctcgcgct tccgatcgtc gggcagcgat tcgaagccgg ccagcgcgac cgtcgcgcag
     1921 gcagcggtcg cgcgccgcaa cggaagctcg gcaacgcgtt cgaacgccgc gcacgggagc
     1981 cacggaatcg cgaaatcggc ggcaccgcgc acgcgcatga cggccgtcgc tccggagcgt
     2041 tcgagcgtca cgcgcggcgc ggcctcgccg gcggcggtaa accattccaa cgaggcgcgg
     2101 ttacgaaacc ggcgatcgag atgccggtgc cagcccggac ggccgcgtac ggt
//
LOCUS       DASUCJ010001018         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_821035, whole genome shotgun sequence.
ACCESSION   DASUCJ010001018 DASUCJ010000000
VERSION     DASUCJ010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821035"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFU90_12055"
     CDS             <1..284
                     /locus_tag="VFU90_12055"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480565.1"
                     /translation="GTIAALKLNRDRMRAAAQSPLLLATAVADAMAAKGTPFREAHER
                     VGRELASIDKLAKEHDVTLQSILAKKSAFGGTSPERVKEAAQKMTERVG"
     gene            281..>1070
                     /gene="argJ"
                     /locus_tag="VFU90_12060"
     CDS             281..>1070
                     /gene="argJ"
                     /locus_tag="VFU90_12060"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEU5480566.1"
                     /translation="MKLPKGFLASGVRAGIRKKRPDLGLIVAEKGATAAAVFTQNRFQ
                     AAPVVLAKSALKKSGGRVKGVVVNAGCANAVTGKPGLDAAKRVQKRAAQLLKCSEDQI
                     FLASTGVIGVVLPDRKVTEALPDSIARLSSGGIDAFSHAILTTDVGPKLAQASFAGNG
                     KRARIVGVAKGAGMIHPNMATMLSFVMTDAALDAKTLHRCLKIAVDQSFNAISVDGDT
                     STNDCVLFMATGQAGEVDVDNFQKALNKVCRELAWMIVRDGEGAT"
BASE COUNT          205 a          327 c          360 g          178 t
ORIGIN      
        1 ccggcacgat cgccgcgctg aagttgaatc gcgaccggat gcgtgccgcc gcgcagtcac
       61 cgctgctgct cgccaccgcg gtcgccgacg cgatggccgc gaagggcacg ccgtttcgtg
      121 aagcgcatga acgcgtcggc cgcgagctcg cttccatcga caaactggcg aaagagcacg
      181 acgtcacgct ccagtcgatt ctggcgaaga agagcgcatt cggcggcact tcgccggagc
      241 gcgtgaagga agccgcacaa aaaatgacgg agagggtggg ttgaagcttc cgaaaggttt
      301 tctcgcatcc ggcgtccgcg ccggcatccg caagaaacgt cccgacctcg gcctcatcgt
      361 cgccgaaaag ggcgcgacgg cagcggcggt gttcacgcag aatcgatttc aggccgcgcc
      421 ggtggtcctg gcgaagtcgg cgctgaaaaa atcgggcggt cgcgtgaagg gcgtcgtcgt
      481 caatgccggc tgtgccaacg cggtgaccgg caagccgggg ctggatgcgg cgaaacgcgt
      541 gcagaagcgc gccgcgcagc ttctcaaatg cagtgaggat caaatctttc tcgcgtcgac
      601 cggcgtgatc ggcgtcgtgc tgccggacag gaaggtcacc gaggcgcttc ccgattcgat
      661 cgcccggctt tcgtcaggcg gcatcgacgc gttctcccac gcgattctga cgaccgatgt
      721 cgggccgaaa ctcgcgcagg cgtcctttgc cggcaacggc aagcgcgcgc ggatcgtcgg
      781 cgtggcgaaa ggcgcgggca tgatccatcc gaacatggcc acgatgctgt cgttcgtgat
      841 gaccgatgcc gctctcgatg cgaagacgct gcatcgctgc ctgaagatcg ccgtcgatca
      901 gagcttcaac gcgatttctg tcgacggcga tacctcgacg aacgattgcg tgctgttcat
      961 ggcaaccggt caggccggtg aagtggacgt cgacaatttt cagaaagctc tgaacaaggt
     1021 ctgccgcgag ctggcgtgga tgatcgtgcg cgacggcgaa ggcgcgacgc
//
LOCUS       DASUCJ010001019         1448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1309703, whole genome shotgun sequence.
ACCESSION   DASUCJ010001019 DASUCJ010000000
VERSION     DASUCJ010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309703"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1448
                     /locus_tag="VFU90_12065"
     CDS             <1..>1448
                     /locus_tag="VFU90_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480567.1"
                     /translation="GGYQGYSAQDSIADGVIIRGQSKVPRRLVFVVNAGTSLPPVALA
                     LSAAGEAAIYTTGGSPAILAPSVAEMRLPYGVRVTYRIADLPDFVQPLRFESVNDPQD
                     AVAKLEAEGAPKTSYPRPAAPAQAIAYPYKNATLPPEPLRMLAVAQIYNVIRYFSPYT
                     KLMHDDWDAAALQAIADERTAPDTRAYLLGLMRFYSHLHDSHGFVEGDLMTEEFGAGV
                     PFETRYLHRQVVVTQPPPSASPMKLGDIIDAVNGVPIRRAMDQIERYISSSTPQAADY
                     AALRPSAQPTVFSGRRGTDVSVTFHRAGGRPQTAVFVREPFIPLPDRRGAKYFVLPGN
                     VGYVDFDRLAPSEVSAMFDALRQTRAIVFDNRGYPRGAAWEVAPRLTAARDVRAALFD
                     TPLVTEPLDAVPEEIQPLPSYKEFYQLLPATTQWRYLKPTVMLIDERAISQSEHSALF
                     FRQATQTIFVGTPTRGANGDVTSMLVPGGVSL"
BASE COUNT          244 a          545 c          415 g          244 t
ORIGIN      
        1 ggcggctatc aaggctactc cgcgcaagac agcatcgccg acggcgttat catccgcggt
       61 cagtcgaaag tgccgcgccg gttggtgttc gtcgtcaacg ccggcacttc gctgccgccg
      121 gtcgccctcg cgctctcggc cgccggcgaa gcggccattt acaccaccgg cggcagcccc
      181 gcgatcctcg cgccgtcggt cgccgagatg cgcttgccgt acggcgttcg cgtcacctac
      241 cgaatcgccg accttcccga cttcgtccaa ccgttacgct tcgaatcggt caacgatccc
      301 caagacgccg tcgcgaaact cgaggccgag ggcgcgccga aaacgagcta cccacgtccc
      361 gccgcgcccg cccaggcaat cgcctacccc tacaaaaacg caacgcttcc gcccgaaccc
      421 ctgcgcatgc tggccgtcgc gcaaatctac aacgtcatcc gttacttctc gccgtacacc
      481 aaactgatgc acgacgattg ggacgccgcc gcgttgcaag cgattgccga cgagcgcacc
      541 gcccccgaca cgcgagccta cctcctcggc ctcatgcgct tttactccca tctgcacgat
      601 tcgcacggct tcgtcgaggg cgacctgatg acggaggagt tcggggccgg cgttccgttc
      661 gagacgcgct acctgcatcg gcaagtcgtc gtcacgcaac ccccgccaag cgcgtcgccc
      721 atgaagctcg gtgacattat cgacgccgtc aacggcgtgc cgattcgccg cgcgatggac
      781 caaatcgaac gctacatcag ctcgtccact ccccaagccg ccgactacgc cgcgttgcgc
      841 ccgtcggccc agccgacggt ctttagcgga cggcgcggca cggacgtgag cgtgaccttc
      901 catcgcgccg gcggccgtcc gcaaaccgcc gtcttcgtcc gcgagccgtt catcccgttg
      961 ccggatcggc gcggtgcgaa gtacttcgtg ctccccggaa acgtcggcta cgtcgatttc
     1021 gaccggctgg cgccgtctga agtgagcgcg atgttcgatg cgctgcgcca aacgcgcgcc
     1081 atcgtgttcg ataatcgcgg ctacccgcgc ggtgcagcct gggaagtcgc tcctcgcctc
     1141 acggccgctc gcgacgtgcg cgcggcgctg ttcgacactc cgctggtcac cgaaccgctc
     1201 gacgccgtgc ccgaagagat tcagccgctg ccctcctaca aggagtttta ccagctgctt
     1261 ccggccacga cgcagtggcg ctatctgaaa cctaccgtca tgctgatcga cgagcgcgcg
     1321 atcagccagt cggaacacag cgccctgttc ttccggcaag ccacgcagac gatcttcgtc
     1381 ggaacaccga cccgcggcgc caacggcgac gtcacctcga tgctcgttcc gggcggcgtg
     1441 tcgctgta
//
LOCUS       DASUCJ010001020         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_759595, whole genome shotgun sequence.
ACCESSION   DASUCJ010001020 DASUCJ010000000
VERSION     DASUCJ010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759595"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="trmFO"
                     /locus_tag="VFU90_12070"
     CDS             <1..891
                     /gene="trmFO"
                     /locus_tag="VFU90_12070"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU5480568.1"
                     /translation="AQRLGAESLAFFDAIAPIVAADSLNHSVLFRASRYGKGDGDDYL
                     NAPLDEAQYDAFVAALVAADQYEPPHEFDRTPYFEGCLPVEEMARRGRDTLRFGPMKS
                     VGLVDPRTGRQPFAVVQLRREDRAGQMWNLVGFQTRLRTGEQRRVFPLIPGLAQAEFL
                     RMGSIHRNSYVNAPATLSPHLAVKDDPLLLFAGQLTGVEGYTESAASGLLAGINLARL
                     LAGEPAVVPPPTTMLGALCHYLTSCEPARFQPMNANFGLLAPLDPPVRDKEKRKAALV
                     ERALRDCDAFAAAIGALVVA"
     gene            903..>1092
                     /locus_tag="VFU90_12075"
     CDS             903..>1092
                     /locus_tag="VFU90_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5480569.1"
                     /translation="MLIAEFVAFLEKERNDSPHTVRAYTRDVEDFAAFCDRYFGGTWS
                     FERIDRLAIRGWLGSMDER"
BASE COUNT          175 a          380 c          358 g          179 t
ORIGIN      
        1 gcccagcgcc tcggcgccga atcactcgcg tttttcgacg ccatcgcacc gatcgtcgcc
       61 gccgactcac tcaatcacag cgtgctgttt cgcgcgtcgc gctacggcaa aggggacggc
      121 gacgattacc tgaatgcgcc gctcgacgaa gcgcaatacg acgccttcgt cgcggcactc
      181 gtcgccgccg accagtacga gccgccgcac gagttcgatc gcacgcctta cttcgagggc
      241 tgcctccccg tcgaggagat ggcccgccgc ggccgtgata cgctgcgctt cgggccgatg
      301 aagtccgtgg gactcgtcga tccccggacg ggacggcagc cgttcgcggt cgttcagctc
      361 cgccgcgagg atcgcgccgg gcaaatgtgg aatctcgtgg gcttccagac gcggctgcgt
      421 accggcgagc agcggcgcgt gtttccactg atcccgggac tcgcgcaggc cgaattcctg
      481 cgaatgggga gcatccaccg caattcgtac gtcaacgctc ccgcgacgtt gtccccccat
      541 ctcgccgtga aggacgaccc gctgctgctc ttcgctggac agctcaccgg cgtcgaggga
      601 tacaccgaat cagcggcgtc gggactgctg gccgggatca acctggcacg gctgctcgcg
      661 ggtgagcccg ccgtcgtacc gccaccgacc acgatgctcg gcgccctctg ccactacctc
      721 acgagctgcg agccggcacg ctttcagccg atgaacgcca acttcgggtt gctcgcaccg
      781 ctcgacccgc cggtccgaga caaagaaaag cggaaggcgg cgctggtcga gcgcgccctg
      841 cgggattgcg acgcgttcgc cgcagcgatc ggcgcgctgg tggtggcgtg accaagccgg
      901 cggtgctgat cgccgagttc gtcgccttcc tggagaagga acgcaacgat tcgccgcaca
      961 cggtgcgggc gtatacacga gatgtggagg acttcgccgc cttctgcgac cgctacttcg
     1021 ggggcacgtg gagcttcgag cggatcgacc gcctcgcgat ccgcggctgg ctcggcagta
     1081 tggacgagcg tg
//
LOCUS       DASUCJ010001021         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_973865, whole genome shotgun sequence.
ACCESSION   DASUCJ010001021 DASUCJ010000000
VERSION     DASUCJ010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973865"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1165
                     /locus_tag="VFU90_12080"
     CDS             <1..>1165
                     /locus_tag="VFU90_12080"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEU5480570.1"
                     /translation="YIVHFPEAKEIISFGSGYGGNALLGKKCLALRIASVMGREQGWL
                     AEHMLILGVESPEGRKHYVTAAFPSACGKTNFAMLIPPPAFKGWKVTTIGDDIAWIKP
                     HKDGKLYAINPEAGMFGVAPGTGYDTNPNCMEAIKADTIFTNVALTPEGDVWWEGQTK
                     EPPPKLIDWTGKEWTPGCGRPAAHPNARFTVSMTRVPSLDPHWDDPDGVPIDAFVFGG
                     RRTTTIPLVTEARDWNDGVYMAATLGSETTAAITGQVGVVRRDPFAMIAFCGYNMSDY
                     FAHWLAFGKKLAKPPKIYLVNWFRKDANGKFVWPGYGENMRVLQWMVGRVEGRAKGTE
                     HAFGTTPSYGDLNWNGLSFTSDQYEQVTSIDPLAWKNELKLHGELFEQLAYHLP"
BASE COUNT          216 a          388 c          391 g          170 t
ORIGIN      
        1 gtacatcgtc cacttccccg aagcgaagga gatcatctcg ttcggctcgg gctacggcgg
       61 caatgcgctg ctcggcaaga agtgcctggc gctgcgcatc gcttcggtga tgggccgcga
      121 gcagggctgg ctcgccgagc acatgctgat cctcggcgtc gagtcgcccg agggcaggaa
      181 gcattacgtg accgcggcgt tcccgagcgc ctgcggcaag accaacttcg cgatgctgat
      241 cccgccgccc gcgttcaagg gctggaaggt gacgaccatc ggcgacgaca tcgcgtggat
      301 caagccgcac aaggacggga agctctacgc gatcaacccc gaggccggaa tgttcggcgt
      361 ggcccccggc accgggtacg acaccaaccc gaactgcatg gaggcgatca aggccgacac
      421 catcttcacc aacgtcgcgc tcacgcccga gggcgacgtg tggtgggaag gccagacgaa
      481 ggagccgccg ccgaagctca tcgactggac cggcaaggag tggacgccgg gttgcgggcg
      541 ccccgccgcg cacccgaacg cgcgcttcac cgtctccatg acgcgcgtgc cctcgctcga
      601 cccgcactgg gacgatcccg acggcgtccc gatcgacgca ttcgtgttcg gcggccggcg
      661 cacgacgacg attccgctcg tcaccgaggc gcgcgactgg aacgacggcg tgtacatggc
      721 cgcgacgctc ggctccgaga ccaccgccgc gatcaccggc caggtgggcg tggtgcggcg
      781 cgacccgttc gcgatgatcg cgttctgcgg ctacaacatg tccgattact tcgcgcactg
      841 gctcgcgttc ggcaagaagc tcgcgaagcc gccgaagatc tacctcgtga actggttccg
      901 caaggacgcg aacggcaagt tcgtctggcc gggctacggc gagaacatgc gcgtgttgca
      961 gtggatggtg gggcgcgtcg agggccgggc gaagggcacc gaacacgcgt tcggcacgac
     1021 gccgagttac ggcgacctga actggaacgg cctgagtttc acgtccgacc agtacgagca
     1081 ggtgacgagc atcgacccgc tcgcgtggaa aaacgagctg aagctgcacg gcgagctgtt
     1141 cgagcaactc gcctatcacc tgccc
//
LOCUS       DASUCJ010001022         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_301106, whole genome shotgun sequence.
ACCESSION   DASUCJ010001022 DASUCJ010000000
VERSION     DASUCJ010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301106"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFU90_12085"
     CDS             complement(<1..754)
                     /locus_tag="VFU90_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480571.1"
                     /translation="MIPNDVSPFAGGLFRHILQSAGVPREVQRKLSIASIVALPLVAW
                     LPLFVLSAVDRRLVPGSVKLPFLLDLSVHIRLLVALPLLLIAAVIAVERLRPTLQQFL
                     TRALVPEPSLARYHAAVASALRLGDSVVADLVMLAAVYSLELFLWRTNLASVASTWRS
                     TPAAAGAHLTPAGLYYVYVSVPVFQFILLRWYYRLIVWARFLLQVAGLRLQLVPTNPD
                     RVGGLGFLLLGTQAFVAFALAHGVLLAAWLSNR"
     gene            complement(767..1270)
                     /locus_tag="VFU90_12090"
     CDS             complement(767..1270)
                     /locus_tag="VFU90_12090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5480572.1"
                     /translation="MPSALDVTTPSEREIRVTRTFAAPPGLVFECHTEPEYVKQWLLG
                     PPGWSMPICEIDLRPGGRYRYVWHNDDNGHEFGVQGEFREIVAPQRIVNVETMDGVPG
                     QALITTTFDEAAGGTLFTLTMQFESQALRDGALESGMTDGMAMSYDRLDGIAANPAMQ
                     DTPLNAP"
     gene            complement(1274..1633)
                     /locus_tag="VFU90_12095"
     CDS             complement(1274..1633)
                     /locus_tag="VFU90_12095"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5480573.1"
                     /translation="MNIALDNERLDATFFALSDPTRRAILVRLAKGEATVNELTESFS
                     ISQPAISRHLKVLEKAQLIRRSRDAQRRPARVETQPIADAVHWIERYRELWETTYQRL
                     DALLAAEVKKRKRRKGD"
     gene            1735..2001
                     /locus_tag="VFU90_12100"
     CDS             1735..2001
                     /locus_tag="VFU90_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5480574.1"
                     /translation="MHEAQAEDQVRTTVSLTRWGDSLAIRIPKAVSDALDLHPGDILD
                     IESGRGQIVLRPRAGLSLDDLIAAITPENVHAAEFECFVGAEVW"
BASE COUNT          387 a          613 c          748 g          348 t
ORIGIN      
        1 cgcggttcga aagccaggcc gccagcagca cgccgtgcgc taacgcgaac gcgacgaacg
       61 cctgcgtgcc caagagcaga aatcccaggc cgccgacgcg gtcgggattg gtcggcacga
      121 gctgcagccg gaggccggca acctgcagca ggaaacgcgc ccacacgatg aggcggtagt
      181 accagcgcag caggataaac tgaaagaccg ggacgctgac gtagacgtag tacagaccgg
      241 cgggcgtgag gtgcgctccg gccgcagccg gcgtcgaacg ccaggtcgag gcgacggacg
      301 cgaggttcgt gcgccagaga aacagttcga gtgagtaaac ggccgcgagc atcacgagat
      361 cggcgacgac ggagtcgccg agacgaagcg cggaggcgac ggcggcgtgg taacgcgcca
      421 gcgacggctc ggggacgagc gcgcgcgtga ggaactgctg caacgtcggg cgcaggcgct
      481 cgacggcgat gaccgcggcg atcaagagca gcggcagcgc cacgagtagc cggatatgca
      541 ccgagaggtc gagcagaaac ggcagcttga cgcttcccgg aaccagccgg cgatcgacgg
      601 cgctgaggac gaacagcgga agccaggcga cgaggggcag cgctacgatc gaggcgatgc
      661 tgagtttgcg ctgcacctcg cgcggcacgc ccgcgctttg caagatgtgc cgaaacaggc
      721 cgccggcgaa gggcgaaacg tcgttaggga tcacgcttcc gcaagcttag ggagcgttta
      781 gcggcgtatc ctgcattgcg gggttggctg cgatgccgtc gaggcggtcg tagctcatcg
      841 ccatgccgtc ggtcatgccg gattccaggg cgccgtcgcg cagcgcttgc gattcgaact
      901 gcatggtcag cgtgaacagc gtgccgccgg cggcttcgtc gaacgtcgtc gtgatgagcg
      961 cttgtcccgg gacgccgtcc atcgtttcga cgttgacgat gcgctgcggc gcgacgattt
     1021 cgcgaaactc gccctgcacg ccgaactcgt ggccgttgtc gtcgttgtgc cagacgtagc
     1081 gatagcggcc gccggggcga aggtcgattt cgcaaatagg catgctccag ccgggcggtc
     1141 cgagcagcca ttgcttgacg tattcgggct cggtgtgaca ttcgaagacg agcccgggcg
     1201 gggcggcaaa cgtgcgggtg acgcgaattt cgcgttccga gggcgtggtg acgtcgaggg
     1261 cggagggcat gggttaatct cctttgcggc gtttgcgttt cttgacttcg gcggcgagca
     1321 gcgcgtcgag ccgttgatag gtggtttccc aaagctcgcg atagcgttcg atccagtgca
     1381 cggcgtcggc gatcggctgc gtctcgacgc gggcgggtcg gcgctgcgcg tcgcggctgc
     1441 gccggatcag ctgcgctttc tcgagcactt tcaggtggcg cgagatggcc ggctggctga
     1501 tcgaaaacga ctccgtcagc tcgttgaccg tcgcttcgcc cttggccaga cgcacgagaa
     1561 tggcacgccg cgtcgggtcg gacagggcga aaaacgtggc gtccaggcgt tcgttgtcga
     1621 gggcgatgtt catagcgcat aggttatata acatagagat tatgaaggcg tcaagggtag
     1681 gccgtggctt aggggatggc ctcggcgcgt agaaggctcc cggcaggatc ctgggtgcac
     1741 gaagcccagg cggaggatca agtgcgcacg accgtatcgc tgaccaggtg gggggatagc
     1801 ctcgccatcc gcattcccaa ggccgtatcg gatgcgctgg acttgcatcc tggagatatc
     1861 ctggatatcg aaagcgggcg cgggcaaatc gtgctaaggc cgcgcgccgg tctctcgctc
     1921 gatgacctaa tcgctgccat cacgcccgag aacgttcatg ccgccgagtt cgaatgtttc
     1981 gtcggcgctg aagtttggta gctgctcgtc cttcgactgc gttaggtgag cgtggctcac
     2041 ctcactccgc tcaggatgac acagcgaagc gcgtggctca cctcactccg ctcagg
//
LOCUS       DASUCJ010001023         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_178909, whole genome shotgun sequence.
ACCESSION   DASUCJ010001023 DASUCJ010000000
VERSION     DASUCJ010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178909"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="VFU90_12105"
     CDS             complement(<1..>1246)
                     /locus_tag="VFU90_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480575.1"
                     /translation="AISKTVNGPADYPFDAFKDLYFDAWQAGLKGIATYRPNDVLGAV
                     LEVASPTAPDAANEDLDATDPDRRIRLDRALEPPLASLRWPGRPELPNGNPCWTYVVR
                     HPLGDFAVFIGHLENGGAGQASATPATGRAWPFEVWINGAEQPRGLGAIAKTLSMDMR
                     THDRAWLDLKLTALQKAAGDDGFEMAMPPDGRKVRVPSLVSGLARLIRYRVEELGAFD
                     GDGHGATPVVDALMGVKEPKAGTDGTLSWTVDVLNPATGDDFVLFLKELIMPDGQRRP
                     YSMWLSGEYPRVLDGLCKALSFDMRVVDPAWIGMKLRKLLNYAEPNEAFMARIPGLPK
                     SQLWPSTIAYLARLMIHRYAMLGILTEEGYPVAHAGILSVPETEQSATALRVLPGRKC
                     PECGNRALIRKDGCDFCTDCGFT"
BASE COUNT          196 a          427 c          421 g          202 t
ORIGIN      
        1 tgtgaagccg cagtccgtgc agaaatcgca gccgtccttg cggatcagcg cgcggttacc
       61 gcattcgggg cacttgcgcc cggggagcac gcgcagcgcc gtggccgact gctcggtctc
      121 ggggacgctc aggatgcccg cgtgggcgac cggatagcct tcttccgtca ggatgccgag
      181 catcgcatag cgatggatca tcagccgcgc gaggtaggcg atcgtcgacg gccacaattg
      241 cgacttcggg aggccgggaa tgcgcgccat gaacgcttcg ttcggctcgg cgtagttgag
      301 aagcttgcgg agcttcatcc cgatccacgc cggatcgacg acgcgcatgt cgaacgacag
      361 cgccttgcac aatccgtcga gaacgcgcgg gtactcgccc gacagccaca tcgaatatgg
      421 ccgccgctga ccgtcgggca tgatcagctc cttcaggaac agcacgaaat cgtcaccggt
      481 ggcggggttc agcacgtcga ccgtccacga cagcgtaccg tcggttcccg ccttcggctc
      541 cttcacgccc atcagcgcat cgacgaccgg cgtcgcgccg tgcccgtcgc catcgaacgc
      601 cccgagctct tcgacgcggt agcggatcaa ccgcgcgagg cccgaaacga ggctcggcac
      661 gcggaccttg cgcccgtcgg gcggcattgc catctcgaag ccgtcgtcgc cggctgcctt
      721 ctgcagcgcg gtgagcttca ggtcgagcca tgcgcggtcg tgcgtgcgca tgtccatcga
      781 caaggtcttc gcgatcgccc cgaggccgcg cggctgctcg gccccgttga tccacacctc
      841 gaacggccac gcgcggcccg tcgccggcgt tgcgctcgcc tgacccgcgc cgccgttctc
      901 gaggtggccg atgaatacgg cgaaatcgcc gaggggatga cgcacgacgt aggtccagca
      961 cgggttgccg ttggggagct cgggccgccc tggccagcgc aggctggcga gcgggggctc
     1021 cagcgcgcgg tcgagccgga tgcgccgatc gggatcggtc gcgtcgaggt cctcgttcgc
     1081 ggcatccggc gcggtcggcg aggcgacttc gagcactgcg cccagcacgt cgttcgggcg
     1141 atacgtcgcg atgcccttca atccggcctg ccaggcgtcg aaatagagat ccttgaacgc
     1201 gtcgaacggg taatcggcgg gaccgttgac cgtcttgctg atcgcc
//
LOCUS       DASUCJ010001024         1424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_26109, whole genome shotgun sequence.
ACCESSION   DASUCJ010001024 DASUCJ010000000
VERSION     DASUCJ010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26109"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFU90_12110"
     CDS             complement(<1..751)
                     /locus_tag="VFU90_12110"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEU5480576.1"
                     /translation="MKVLILGVNGFIGNALTERILTTTDWEVFGLDMSAEKIEPFLDN
                     RRFRFLEGDIAINKEWIEYHIKKCDVVLPLVAIATPATYVRDPLAVFELDFEENLRIV
                     KQCVRYKKRVVFPSTSEVYGMCPDPEFDEYRSTLVYGPIHKQRWIYSTSKQLLDRVIF
                     AYGQQEGLEFTLFRPFNWIGPHLDNVDSPKEGSSRVLTQFLHNILYGEPIKLVDGGRQ
                     RRSFTHIRDGIDCLMRILVNEQGVANGGIFNI"
     gene            complement(748..>1424)
                     /locus_tag="VFU90_12115"
     CDS             complement(748..>1424)
                     /locus_tag="VFU90_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEU5480577.1"
                     /translation="PLAALAVAPLGSFNVHGSLLPRYRGRAPVNWAVLHGETETGATL
                     HVMTARPDAGDIVDQETVPIGPDDTASEVQRRVCDAAVHVLERQLDALAAGRAPRRPQ
                     DAARATTFGKRRPEDGGFDWSRPAAEIHNLVRAVSHPYPGAFTPHGGRTLHVWRTRNG
                     GWSANGAHPGEMRVIDDRLYVACGGPDTRWLEIVRGQIEGDVERDGADLARHLALHTS
                     TTPPAP"
BASE COUNT          220 a          444 c          507 g          253 t
ORIGIN      
        1 cgatgttgaa gatcccgccg ttggctacgc cctgttcgtt caccaggatg cgcatcaagc
       61 aatcgatgcc gtcccggatg tgcgtgaacg accgccgctg gcgtccgccg tccacgagtt
      121 tgatcggttc gccgtagaga atgttgtgca agaactgcgt gaggacgcgc gacgagcctt
      181 ccttgggcga gtcgacgttg tccaggtgcg ggccgatcca gttgaacgga cggaacagcg
      241 tgaactcgag tccctcttgc tggccgtagg cgaagatcac gcggtcgagc aattgcttgc
      301 tcgtcgagta aatccaccgc tgcttgtgaa tcgggccgta cacgagcgtc gatcgatact
      361 cgtcgaactc gggatccgga cacatgccgt acacttccga cgtcgacggg aacacgacgc
      421 gcttcttgta gcgcacgcat tgcttcacga tgcgcaggtt ctcctcgaag tcgagctcga
      481 acaccgccaa cggatcgcgc acgtacgtcg cgggcgtggc gatcgcgacg agcgggagca
      541 cgacatcgca cttcttgatg tggtactcga tccactcctt gttgatcgcg atgtcgccct
      601 cgagaaaccg gaagcggcgg ttgtcgagga acggctcgat cttttcggcg gacatgtcga
      661 gcccgaacac ctcccagtcc gtggtggtga ggatgcgttc ggtgagcgcg ttgccgatga
      721 acccattcac gccgaggatg agaactttca tggggctgga ggagtggtgg acgtgtggag
      781 cgcgaggtgg cgcgcgaggt ccgcgccgtc gcgctcgacg tcgccctcga tctggccgcg
      841 tacgatctcg agccaccgcg tgtcgggacc gccgcacgcg acgtagaggc ggtcgtcgat
      901 cacgcgcatc tcgcctgggt gggcgccgtt cgcactccag ccgccattgc gcgtgcgcca
      961 gacgtgcagg gtgcgtccgc cgtgcggcgt gaatgcgccc gggtacgggt gcgatacggc
     1021 gcgcacgaga ttgtggatct cggcggccgg acggctccag tcgaagccgc cgtcttcggg
     1081 gcggcgcttg ccgaacgtcg tggcacgggc ggcgtcctgc ggcctgcgcg gcgcgcgtcc
     1141 cgcggcgagg gcgtcgagtt ggcgctcgag cacgtgcacg gcggcgtcgc atacgcggcg
     1201 ctgcacctcg gaggccgtgt cgtcggggcc gatgggcacc gtctcctggt cgacgatgtc
     1261 gccggcgtcc gggcgcgcgg tcatcacgtg gagggtggcg ccggtttccg tttcgccgtg
     1321 gagcacggcc cagttgacgg gcgcgcgtcc gcgatagcgc gggaggagcg agccgtgcac
     1381 gttgaagctg cctaacggcg cgaccgccag cgccgcgagc ggca
//
LOCUS       DASUCJ010001025         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_316232, whole genome shotgun sequence.
ACCESSION   DASUCJ010001025 DASUCJ010000000
VERSION     DASUCJ010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316232"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFU90_12120"
     CDS             <1..257
                     /locus_tag="VFU90_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480578.1"
                     /translation="TAATAIAAIGNAFPRGAWLTSATMKPSLALGLKGRADTIGSLET
                     VVRELVKNRSGSRPSGLRFSREGRDQLAELEFELQLGGHR"
     gene            254..838
                     /locus_tag="VFU90_12125"
     CDS             254..838
                     /locus_tag="VFU90_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480579.1"
                     /translation="MTRSTAAIDVFVAWLLVLAVAIAGYGGVVVPVARRARATMSEAQ
                     DRYDAANRNLRILANASNLEAARARVSRDLAALANEQRQGSATAALLRLLRRVGKQNE
                     VLVTALSPAQRPDGGPETEAATIVLRGAYSSVLRAVAALSRDGPIVEVRELALAQTTG
                     DFGMPEVEATVGVSMYRRIAAPGKGRGEVGNEGS"
     gene            819..1268
                     /locus_tag="VFU90_12130"
     CDS             819..1268
                     /locus_tag="VFU90_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480580.1"
                     /translation="METRGRNGWSWLLAFGAAAMWAIGGGTLGSYAEEGLVPPGSLQI
                     PKRLVFPPIVVTRDPFRVEELVPPLPAVGGDTPSDLVLPPNAAVAGPRLRAVIAGGPP
                     RALIEENGRTRIVGVGSELAGSVVSEISESEVRLEDGETLRLTGLQP"
     gene            1274..>2126
                     /locus_tag="VFU90_12135"
     CDS             1274..>2126
                     /locus_tag="VFU90_12135"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU5480581.1"
                     /translation="MAAFVLAAALCASGGASAAEKLLSIDVRDVDIGDVIALLSAQSG
                     TNVVTDGSVKPQRVTMHLHGVTFGEALRAIAGAHGLQVRREGGILIVGSAETMNRRGQ
                     STVLAVRHAATDDVARELGDALPEGTVVVADKRSGNVIVEGDATTLAHARTLVGELDS
                     SSGSGDGGTLPTRSYRLRYTRPDDAVAKLKALVSGGAFLADDQQNAVLVTGAEGVQAN
                     ARGVIATIDRAAPQVLFEVKVADVTPINDASNFGIEFGGLDLQGQPVPGAITYSFAGG
                     TIPVNVRL"
BASE COUNT          362 a          661 c          753 g          350 t
ORIGIN      
        1 cgacggcggc caccgcgata gccgcgatcg gcaacgcctt cccccgcggc gcttggctga
       61 cgtcggcgac gatgaagccg tccttggcgt tggggttgaa aggccgcgcc gatacgatcg
      121 gcagtctgga aaccgtcgtg cgcgagctgg tgaagaatcg aagcggcagc cgtccgagcg
      181 gcttgagatt ctcgcgagaa gggcgcgacc agctcgccga gctggagttc gagctgcagc
      241 tgggaggaca tcggtgacgc gctcgaccgc tgccatcgac gtgttcgtcg cctggctttt
      301 ggtcctcgcc gtggcaattg ccggatacgg cggggtcgtc gttccggtcg cccggcgggc
      361 gcgggcgacg atgagcgaag cgcaggatcg ctacgacgca gcgaatcgca atctcagaat
      421 tctcgccaac gcctcgaatc tcgaagcggc ccgcgcacgg gtctcgcgcg atctcgcggc
      481 attggcaaac gagcaaaggc aggggagcgc taccgcggcg ttgctgcgcc ttctgcgccg
      541 cgtaggtaaa caaaacgaag tgctcgtgac ggcgctgtct cccgcgcagc gtccggacgg
      601 cggcccggaa accgaagccg caacgatcgt cctgcggggg gcttactcca gcgtcttgcg
      661 cgccgtcgca gcgctttcgc gcgacggacc catcgttgaa gtccgcgagc tcgcgctcgc
      721 acaaacgacg ggggacttcg gaatgcccga agtcgaggcg accgttggag tatcgatgta
      781 tcgccgcatc gcggcgcctg gaaaggggag gggcgaagtt ggaaacgagg ggtcgtaacg
      841 gctggtcgtg gctgctcgcg ttcggcgcgg cggcgatgtg ggcgatcggc ggcgggacgt
      901 taggctcgta cgccgaggaa ggcttggtgc cgccgggatc gctgcagatt ccaaagcggc
      961 tcgtcttccc gccgatcgtc gtcacccgcg atccgtttcg agtggaggaa ctggttccgc
     1021 cgttaccggc ggtcggcggc gacactccat cggatctcgt gctgcctccg aatgccgccg
     1081 tcgccggacc gagacttcgc gcggtaatcg ccggcggacc gccgcgggcg ctgattgaag
     1141 agaacggccg aactcgaatc gtcggcgtcg gcagcgagct cgcgggttcg gtcgtcagcg
     1201 agatctcgga aagcgaagta cggctcgagg acggtgaaac gttacggctg acggggttgc
     1261 aaccgtgatt cgtatggctg cgttcgtgtt ggctgcggcg ttgtgcgctt ccggcggcgc
     1321 ctcggccgcc gaaaaactgc tgtcgatcga cgttcgcgac gtcgatatcg gcgacgtcat
     1381 cgcccttttg tcggcgcagt ccgggaccaa cgtcgttacc gacggatccg tgaagccgca
     1441 gcgcgtaacg atgcacctgc acggggtgac cttcggcgaa gctttgcgtg cgattgccgg
     1501 ggcgcacggg ctgcaggtgc ggcgggaggg cggcatcttg atcgtcggga gcgccgagac
     1561 gatgaatcgt cgcggccaga gcaccgtgct ggcggtacgg cacgccgcaa ccgacgacgt
     1621 cgccagagag ctcggcgacg cactgcccga ggggacggtc gtcgttgcgg acaaacgctc
     1681 cgggaacgtg atcgtggagg gcgacgcgac gacgctagcg cacgcccgca ccctggtcgg
     1741 cgaactcgat tcgtcttccg gaagcggcga cggcggaacc ctgccgacgc gatcgtaccg
     1801 tcttcgctat acccggcccg acgacgccgt cgccaagctg aaggcactcg tgagcggagg
     1861 cgccttctta gcggacgacc agcaaaacgc cgtgctcgta acgggcgcgg aaggcgttca
     1921 agccaacgcg cgcggcgtta ttgccactat cgatcgtgcc gccccgcaag tactgttcga
     1981 ggtcaaagtc gcggacgtaa cgccgatcaa cgatgcgagt aacttcggca tcgagttcgg
     2041 cggcctcgat ctgcaaggac agccggtacc gggagcgatc acgtattcgt tcgccggcgg
     2101 cacgattccg gtcaacgtgc ggctca
//
LOCUS       DASUCJ010001026         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_973900, whole genome shotgun sequence.
ACCESSION   DASUCJ010001026 DASUCJ010000000
VERSION     DASUCJ010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973900"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VFU90_12140"
     CDS             <1..1317
                     /locus_tag="VFU90_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5480582.1"
                     /translation="VIAHTPIRHVVVTSIGEFLAIKGKLVDLVLRRVKKMIPKWSLPG
                     SIRLNDAMRQGRKLALARVPIAHADLAFLQYTGGTTGVAKGAMLTHGNMVANMLQARA
                     WVRPFLSAAGQREVIMTPLPLYHVFSLTANCLVFMSLGGENVLITNPRDIPRFVREMR
                     RYRFTAMTGVNTLFNALLNHPRFASLDFSSLRLTLGGGMAVQEAVARRWKEVTGVTLV
                     EAYGLTETSPAATINPLDIEAFNGTIGLPIPSTWVTLRDDDGHDVPLGQSGEICIRGP
                     QVMAGYWQRPDETANVMTADGYLKSGDIGNMNERGFVRIVDRKKDMILVSGFNVYPNE
                     IEGVVAEHPGVLECAAVAIPDPKSGEAVKLYVVPKDPTLTVDEMMSFCRERLTGYKCP
                     REIEFRRELPKSNVGKILRRTLRDEARAEVAVRSAKIAAVQVGSAQ"
     gene            1314..1715
                     /locus_tag="VFU90_12145"
     CDS             1314..1715
                     /locus_tag="VFU90_12145"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU5480583.1"
                     /translation="MSEGSRSVTLPPGIPALRVAPMPADANFHGDIFGGWIMSQADIA
                     GSVPASRRARGRIATVAVNSFVFKEPVLIGDVVSFYATIQKVGRTSITVAVEVYAQRN
                     PREEITVKVTEASLTYVAVGPDRKPRALPPE"
     gene            1850..>2355
                     /locus_tag="VFU90_12150"
     CDS             1850..>2355
                     /locus_tag="VFU90_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU5480584.1"
                     /translation="MHERFLKRSQIATIVGSIALGFAAGQAWGAGFQLNETSASGLGN
                     AFAGGAAEAQDATTVWSNPAGMSKLTSPQVAAVVNLVTPSFKFQNDGSQPAAFQPLGN
                     SGGDAGSLNVVPNLYAVMPINHDWSVGLGVNVPFGLVTEWGNGWIGRFQALKSDVKTI
                     NVNPSVSWR"
BASE COUNT          410 a          772 c          794 g          379 t
ORIGIN      
        1 gtgatcgcgc acacgccgat ccgtcacgtg gtggtgacgt cgatcggtga gttcctcgca
       61 atcaagggca agctcgtcga cctcgtgctg cgccgggtca agaagatgat cccgaagtgg
      121 tcgctgccgg gctcgatccg gctgaacgac gcgatgcgcc agggccgcaa gctcgcgctg
      181 gcgcgtgtgc cgatcgcgca cgccgacctc gcctttctcc agtacacagg cggaacgacc
      241 ggtgtcgcca agggcgcgat gttgacgcac ggcaacatgg tcgcgaacat gctgcaggcc
      301 cgcgcgtggg tgcgcccgtt cctgagcgcg gccggccagc gcgaggtcat catgaccccg
      361 ctgccgctct accacgtgtt ctcgctcacc gcgaactgcc tcgtgttcat gtcgctcggc
      421 ggcgagaacg tgctgattac caatccgcgc gacattccgc gcttcgtgcg cgagatgcgc
      481 cgctatcgct tcaccgcgat gaccggcgtc aatacgctgt tcaacgccct gctcaaccat
      541 ccgcgcttcg cgtcgctcga cttctcgtcg ctgcgcctca cgctgggcgg cggcatggcg
      601 gtgcaggagg cggtcgccag gcgctggaag gaggtgaccg gggtcacgct ggtcgaagcc
      661 tacgggctca ccgagacctc gccggccgcg acgatcaatc ctctcgacat cgaggcgttc
      721 aacgggacga tcggcctgcc gattccgtcg acgtgggtca cgctgcgcga cgacgacggc
      781 cacgacgtgc ctctcgggca atccggcgag atctgcatca gggggccgca ggtgatggcg
      841 ggctactggc aacggcccga cgagacggcg aacgtaatga ccgccgacgg ctacctcaag
      901 agcggcgaca tcggcaacat gaacgagcgc ggcttcgtgc gcatcgtcga tcgcaagaag
      961 gacatgatcc tcgtttcggg cttcaacgtg tatccgaacg agatcgaggg cgtcgtcgcc
     1021 gagcaccctg gggtgctcga atgcgcggcg gtcgcgatac ccgatccgaa gtcgggcgag
     1081 gcggtgaagc tctacgtcgt gccgaaggat ccaacgctca ccgtcgacga gatgatgagc
     1141 ttctgccgcg agcggctgac cggctacaag tgcccgcgcg agatcgagtt caggcgcgag
     1201 cttcccaagt ccaatgtcgg caagatcctg cgccgcacgc ttcgcgacga ggcgcgcgcc
     1261 gaggtggccg tgcgcagcgc gaagatcgcc gccgtgcagg tcgggagcgc gcagtgagcg
     1321 agggctcgcg gagcgtcacg ctgccgccgg ggatcccggc gttgcgcgtc gcgccgatgc
     1381 cggcggatgc gaacttccac ggcgacatct tcggcggctg gatcatgtcg caggccgaca
     1441 tcgccggctc ggtgccggca tcgcgccgcg cgcgcgggcg catcgcgacc gttgccgtca
     1501 attcgttcgt gttcaaggag ccggtgctga tcggcgacgt ggtctcgttc tacgcgacga
     1561 tccagaaagt cggccgcacg tcgatcaccg ttgcggtcga ggtgtacgcg cagcgcaatc
     1621 cgcgcgagga gatcaccgtc aaggtgaccg aggcgagcct cacctacgtc gcggtcgggc
     1681 ccgaccgcaa gccgcgcgcg ctgccgccgg aatgattctc acctgcgaac ggcacgtgca
     1741 cgggaaaata tcctggggaa atgtcaaaat tctgcaagcg atgaagcggt atagtcacgc
     1801 ggcgaagggc cgcacgcgga caaccaaccg atcaaggaga gcatcagtca tgcacgagcg
     1861 attcctgaag cggagccaga tcgccacgat cgtcggcagc atcgccctcg gcttcgccgc
     1921 cgggcaggca tggggcgccg gattccagtt gaacgagact tcggcctccg ggcttggcaa
     1981 cgcgttcgcc ggcggcgctg cggaagcgca ggacgccacc accgtgtggt cgaatccggc
     2041 cggcatgtcg aagctcacgt caccgcaggt tgcggcggtc gtgaacctcg tgacaccgtc
     2101 gttcaagttc cagaacgacg gttcgcagcc cgcggcgttc cagccgctcg gcaattcggg
     2161 cggcgacgcc ggcagcctca acgtcgtgcc gaacctctac gcggtgatgc cgatcaacca
     2221 cgactggagc gtcggcctcg gcgtcaacgt tccattcggc ctcgtgaccg agtggggcaa
     2281 cggctggatc ggtcgcttcc aggcgctgaa atccgacgtg aagacgatca atgtcaatcc
     2341 gtcggtgtcg tggcg
//
LOCUS       DASUCJ010001027         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_973903, whole genome shotgun sequence.
ACCESSION   DASUCJ010001027 DASUCJ010000000
VERSION     DASUCJ010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973903"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU90_12155"
     CDS             complement(<1..306)
                     /locus_tag="VFU90_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5480585.1"
                     /translation="MQQTVSQRARALIELEERHGAHNYHPLDLVVDRAEGVWLWDVDG
                     KMYLDCVSAYSAVNQGHCHPRIRAALVEQSAKVTLTSRAMRNDRMPGFLEKLTHVCGF
                     "
     gene            419..1303
                     /locus_tag="VFU90_12160"
     CDS             419..1303
                     /locus_tag="VFU90_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5480586.1"
                     /translation="MGTSSIRLERLLARDHAGVGRKGRTIAHLHGERRPRAIVLLHGM
                     SSSPPQFERFAHDLYRRGHNVLVPRLPGHGHADRFSTALAQLRPDDLYAAVDEYVAIA
                     RELGERVTFAGFSLGGLLTAWSAQHYELDRCVAIAPFFGVAWIPGGLMSAVAELLLRI
                     PNQFHWWDPVLRERQQPEHGYPRYATHAIAHSYRIARTLLIEAAGRPPSAAHVVLVTN
                     ARETAVDNRAIRRLYARWSAQRPDAVELIVLTGLPLSHDIVEPLRRGDLAARAYPDLL
                     SAIDPPTGDSASSSSGDR"
     gene            complement(1251..>1693)
                     /locus_tag="VFU90_12165"
     CDS             complement(1251..>1693)
                     /locus_tag="VFU90_12165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5480587.1"
                     /translation="GLSWETILRKRDGYRRVFLDFDPQRVARLRAPAIERILRDPGIV
                     RNRAKVESTLGNARAFLRVARTHGTFARFAWEFVGGRPIVNSRRSSAQIPATSEQSEA
                     MSRALRALGFRFVGPTICYAFMQAVGMVNDHLTSCWRYRPLADR"
BASE COUNT          264 a          575 c          565 g          289 t
ORIGIN      
        1 gaatccgcag acgtgcgtca gtttctccaa gaagcccggc atgcggtcgt tgcgcatcgc
       61 gcgcgacgtc agcgttactt tggcggattg ttcgaccaac gcggcgcgaa tacgcggatg
      121 gcagtgtccc tgattgaccg ccgagtacgc actgacgcag tcgaggtaca ttttgccgtc
      181 gacgtcccag agccagacgc cctcggcccg gtcgaccacc agatcgagcg ggtgatagtt
      241 gtgcgcgccg tggcgctcct ccagctcgat gagagcgcga gcgcgttgcg aaaccgtctg
      301 ttgcattgcc gtcgtcctag gcgccgcgtg tgtttaccgg cccgggggcc gggaccgcgg
      361 gggcgcaaat cgcccgcgtt cgaggaacac ttgccggtct gacttaagga cgcttcgctt
      421 gggaacctct tcgattcgcc tcgagcgcct gctcgcgcgg gatcacgccg gcgtcggtcg
      481 caagggtcgc acgatcgctc acctgcacgg ggaacggcgt ccgcgcgcga tcgtgctgct
      541 gcacggcatg agctccagcc cgccgcaatt cgagcgtttc gcgcacgact tgtaccggcg
      601 cggtcataac gtcctggtgc cgcggctgcc ggggcacggg catgcggatc ggttttcgac
      661 cgcgctggca caactgcgcc ccgacgacct ctacgccgcc gtcgacgagt acgtcgcgat
      721 cgcgcgcgaa ctcggcgaac gcgtgacgtt tgccggcttt tcgctgggcg gattgctgac
      781 cgcctggagc gcgcagcact acgagctgga ccggtgcgtc gcgatcgcgc cgtttttcgg
      841 cgttgcgtgg attcccggcg ggctgatgtc ggcggttgcc gagctgctgc tgcgcatccc
      901 gaatcaattt cattggtggg atccggtcct gcgcgagcgc cagcagcccg agcacggata
      961 cccgcgctac gccacccacg cgatcgcgca ctcctaccgg atcgcgcgga cactgctgat
     1021 agaggcggcg ggccgcccgc cgtcggccgc ccacgtcgtg ctcgtgacca acgcgcggga
     1081 aaccgccgtc gacaaccgcg cgatccggcg gctgtacgcg cgctggtcgg cgcagcgtcc
     1141 ggacgcggtc gagctgatcg tccttacggg tttgccgctg tcgcacgata tcgtcgaacc
     1201 gctgcggcgc ggcgatctgg ccgcgcgcgc gtaccccgat ctgctctcgg ctatcgatcc
     1261 gccaacgggc gatagcgcca gcagctcgtc aggtgatcgt tgaccatccc gaccgcctgc
     1321 atgaaggcgt aacaaatcgt cggaccgacg aagcgaaacc cgagcgcgcg cagcgcccgg
     1381 ctcattgcct cggattgctc gctggtcgcg ggaatttgcg ccgagctgcg gcgcgaattg
     1441 acgatcggcc ggccgccgac gaactcccag gcaaaccgcg cgaacgtccc gtgcgttcgc
     1501 gcgacgcgta aaaatgcgcg cgcgttgccg agcgtggatt cgaccttcgc acgattgcgg
     1561 acgattcccg gatcccgcaa aatccgttcg atcgcgggcg cgcgcaagcg ggcgacgcgc
     1621 tgcggatcga agtcgagaaa cacgcggcgg tagccgtcgc gcttgcgcaa aatcgtttcc
     1681 cagctcaagc cgg
//
LOCUS       DASUCJ010001028         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1416995, whole genome shotgun sequence.
ACCESSION   DASUCJ010001028 DASUCJ010000000
VERSION     DASUCJ010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VFU90_12170"
     CDS             complement(<1..1053)
                     /locus_tag="VFU90_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5480588.1"
                     /translation="MDFDDLLVLPVRLLRENPAQLAAYRDRFRHILVDEYQDTNRAQY
                     QFVRLLASDAGNVCVVGDDDQSIYGWRGADIRNILDFTKDFPSAKIVRLEENYRSAPP
                     ILEVANVVIRANTGRMGKTLRPTRTGGEPVTSLRSLDERDEADWIIEEFMTRRRTKNL
                     ELRDFAVLYRTNAQSRALEEALRRHAIPYRLVGAVRFYDRREIRDLMSYLKLIANPSD
                     DEAFRRAVAVPKRGLGETSLDTLAEAARQEHLSLFAAAGRGELLASIRPAARGALSQF
                     VALIERLRAQAENAAVDELLRQVVDDIRYGDYLKAEGPESADRLENVKELITGAAELV
                     ADELGEVGLRPLDHFLQ"
BASE COUNT          198 a          352 c          320 g          193 t
ORIGIN      
        1 ctgcaggaaa tggtcgagcg ggcgaagtcc gacttccccg agctcgtcgg caacgagctc
       61 cgcggcgccg gtgatcagct ccttcacgtt ctccagccga tccgccgatt ccgggccctc
      121 ggccttgaga tagtccccat agcgaatgtc atccacgact tggcgcagca gttcgtccac
      181 cgccgcattc tcggcctgcg cacgcaaccg ctcgatcaac gcgacgaact gcgagagcgc
      241 accccgtgct gccgggcgaa tcgaggctag cagctcaccg cgacccgcag ccgcaaacag
      301 cgataaatgc tcctgccgcg ccgcctcggc cagcgtatcg agtgaggttt ctccgagtcc
      361 ccgcttcgga actgcaaccg cacgccggaa ggcttcgtca tccgacggat tcgcgatgag
      421 cttgagatag ctcatgagat cacgaatctc ccggcgatcg tagaagcgaa ccgcgccgac
      481 gagtcggtac gggatggcgt gacggcgcag cgcctcctcg agcgcgcggc tctgcgcgtt
      541 cgtgcggtac aatacggcga aatcccgtag ctcgaggttc ttcgttcggc gccgcgtcat
      601 gaactcttcg atgatccaat cggcttcatc gcgctcgtcg agcgaccgca gcgacgtcac
      661 tggctcgccg ccggttcgcg tcggccgcag agtcttcccc atgcgaccag tattggcgcg
      721 aatgacgacg ttcgcgactt ccaggatcgg cggtgccgac cgataattct cctcgagcct
      781 tacgattttc gccgagggaa agtccttcgt gaaatcgaga atgttgcgga tgtccgcgcc
      841 gcgccaacca taaatggatt gatcgtcgtc tccgaccacg cagacgttcc cggcgtcgct
      901 ggcgagcagt cgcacgaact gatactgcgc gcggttggta tcctggtact cgtcaacgag
      961 gatatggcgg aagcggtcgc gatacgcggc gagctgcgcg ggattctccc gcagcaacct
     1021 gacgggaagc acgagcaagt cgtcgaaatc caccgcgttc gcg
//
LOCUS       DASUCJ010001029         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_698361, whole genome shotgun sequence.
ACCESSION   DASUCJ010001029 DASUCJ010000000
VERSION     DASUCJ010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698361"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFU90_12175"
     CDS             <1..720
                     /locus_tag="VFU90_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480589.1"
                     /translation="GFAGPFGECTDAAGDVFVTNAKPPEILEFVHGGTAPIATLKDPG
                     QYPYSCSVDPTTGNLAVTNEYARNSTPGSVAIYRHARGTPKLYYDSAFYYMFFCGYDN
                     AGNLFVDGEPKPSGGFSFAELPHGAAKLRNVTLNVAIGFQGGVQWDGKHIAVGDQIGS
                     TIHRFTFSGKNGTEVGSTQLLNAKQIVQFGKQGAYVAGPDAIYYRVGIWSYPAGGNPI
                     RFLNYSFGLPVAATVSKVSTP"
     gene            769..918
                     /locus_tag="VFU90_12180"
     CDS             769..918
                     /locus_tag="VFU90_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480590.1"
                     /translation="MRSRLGLYTALGAAIGVAIGAGSHHVAQGLALGVAFGVAIGALS
                     DRISR"
BASE COUNT          181 a          300 c          388 g          207 t
ORIGIN      
        1 gggttcgcgg ggccgttcgg ggagtgcacc gacgcggcgg gcgacgtgtt cgtcaccaac
       61 gcgaagccgc cggagattct cgagttcgtg cacggcggca cggcgcccat cgcgacgctc
      121 aaagatccgg gacagtatcc atattcgtgt tcggtcgatc cgacgaccgg caatctggcg
      181 gtgaccaacg agtacgcccg gaactcgacg cccggcagcg tcgcgatcta ccggcacgcg
      241 cgcggcacgc caaagctgta ttacgattcc gcgttctact acatgttttt ctgcgggtac
      301 gacaacgccg gcaacctgtt cgtcgacggg gaaccgaagc cgagcggcgg attttcgttc
      361 gccgagcttc cgcacggcgc tgcgaagttg cgcaacgtca cgctcaacgt tgcgatcggt
      421 tttcagggcg gcgttcaatg ggacggcaag catatcgcgg tgggcgatca aatcgggtcg
      481 acgattcacc ggtttacgtt ttccggtaag aacggaacgg aagttgggtc gacgcagctg
      541 ctcaacgcca agcagatcgt ccagtttggg aagcagggcg cgtacgtcgc cgggccggat
      601 gcgatttact accgggtggg gatttggagc tatcccgccg gcggtaatcc gatccggttc
      661 ctcaactaca gcttcggctt gccggtggcg gccacggtca gtaaggtttc gacgccctga
      721 gcggtgccga cgcgtgggac gtcgtgcggg aatgactgga gcaaacggat gaggtcgcgg
      781 ttgggcttgt atacggcgct gggtgcggcg atcggagtgg cgatcggcgc cggttcgcat
      841 cacgtcgcac aggggctcgc gctgggcgtc gccttcggcg tggcgatcgg tgcgctgagc
      901 gatcgcatct cgcggtgaag gaggggacat gcgctggttg gcgcgtctcg gcgcagttgc
      961 gttgacgttg gggttcggaa gtgcggcggt tgcacagacg aacgtgccag cggcgcggat
     1021 tctcggcgga ttagaggaga ggttctatct gtccggtcag ccgcgggttg tacgga
//
LOCUS       DASUCJ010001030         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_378107, whole genome shotgun sequence.
ACCESSION   DASUCJ010001030 DASUCJ010000000
VERSION     DASUCJ010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378107"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1273
                     /locus_tag="VFU90_12185"
     CDS             251..1273
                     /locus_tag="VFU90_12185"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5480591.1"
                     /translation="MKIAFSRREAIALAVAAGLVGGAQIAYAAETTLLNVSYDPTREL
                     YRDFNAAFVAKWKQDTGETLKINQSHGGSGKQSRSVIDGLEADVVTLALAYDIDALAG
                     HGGLVPKDWQQRLPQNSAPYTSTIVFLVRKGNPKGIKDWDDLGKPGVGVITPNPKTSG
                     GARWNYLAAWGFAAKKYGGDAGAKDFVAKVYRNVLVLDTGARGATTTFVERGQGDVLI
                     AWENEAILALGESGGAGFEIVAPSVSILAEPPVSVVDKVVDKRGTRKVAEAYLKYLYS
                     PEGQEIAAKDGYRPRDATVAAKYASKFPRLSMFTVDEAFGGWQKAQATHFADGGTFDQ
                     IYAPGR"
BASE COUNT          261 a          406 c          413 g          202 t
ORIGIN      
        1 agcagcagct catttgttag aagaagatac tcatttccag ggagcttgtg cgcgcgtccc
       61 cgcttgtaag gtcttgcgta gtaaacaagc cttttggtcc gaacaaacgc gccagacccg
      121 cttgcaggcc tactcaacaa aagcgaataa gctcagcaga tcttcttggt tcgtggtgaa
      181 cgctggcgca gctaggtgtt cctcgtaccc tttcaacggg tgaggtcacg aacacgagag
      241 gatgctccta atgaaaatcg cattttcccg ccgcgaggca atcgcactcg ccgttgcggc
      301 cggcctagtc ggaggcgccc agatcgccta cgccgccgag acgactctgc tcaacgtctc
      361 ctacgatccg acgcgcgagc tctaccgcga cttcaacgcg gcgttcgttg cgaagtggaa
      421 gcaggacacg ggcgagacgc tgaagatcaa ccagtcgcac ggcggctcgg gcaagcagag
      481 ccggagcgtc atcgacgggc tcgaagccga cgtcgtcacg ctcgcgctcg cctacgacat
      541 cgacgcgctc gccgggcacg gcgggctcgt gccgaaggac tggcagcagc gccttccgca
      601 gaacagcgcg ccgtacacgt cgacgatcgt gttcctcgtg cgcaagggca atccgaaagg
      661 catcaaggac tgggacgatc tcggaaaacc cggtgtcggc gtcatcacgc cgaacccgaa
      721 gacgagcggc ggcgcgcgct ggaactacct ggccgcgtgg ggcttcgccg cgaagaaata
      781 cggcggcgac gcgggcgcga aggatttcgt cgcgaaggtc tacaggaacg tgctcgtgct
      841 cgacaccggc gcgcgcggcg cgacgacgac attcgtcgag cgcggccagg gcgacgtgct
      901 gatcgcgtgg gagaacgaag cgatcctcgc gctcggcgaa tcgggcggcg ccggtttcga
      961 gatcgtcgca ccgtcggtca gcatcctcgc cgagccgccg gtcagcgtgg tcgacaaggt
     1021 cgtcgacaag cgcggcacgc gcaaggtcgc cgaggcctat ctcaaatacc tctactcgcc
     1081 cgaaggccag gaaatcgcgg cgaaggacgg ctatcgcccg cgcgacgcca ctgtcgccgc
     1141 gaaatatgcg agcaagttcc cgcgactatc gatgttcacg gtcgacgaag cattcggcgg
     1201 ctggcagaag gcgcaggcga cgcacttcgc cgacggcggc acgttcgatc agatctacgc
     1261 gccgggacga tgacgttgcg ca
//
LOCUS       DASUCJ010001031         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_744530, whole genome shotgun sequence.
ACCESSION   DASUCJ010001031 DASUCJ010000000
VERSION     DASUCJ010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744530"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>1032)
                     /locus_tag="VFU90_12190"
     CDS             complement(130..>1032)
                     /locus_tag="VFU90_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480592.1"
                     /translation="VAYDVGRATLFGDVQVRRAQWRYTPDVHADVAPASISWTFVNPR
                     GGLTYRLAPSLSLYASYGTMSREPARNDMLAGFDNLDTSNVAFVGPLSRVKPETVHDI
                     ESGVNVRTGALRFEGNIFSMDFRNEIEPIGALSYLGLPLRKNVRASYRRGVELDAGYM
                     LPAGFSASANATIMNAKIADYTDDSSGETFHDVEPLLTPKVTTAQRLTWQGTRRFTIS
                     LGGRYTGRSQLDNTGNPNMVLPSSYVMDGLFEWHFAHGRHALALRGENLTNSKRYGSG
                     YSGDRPYYYVLQPRSLFATLELGF"
BASE COUNT          174 a          364 c          317 g          177 t
ORIGIN      
        1 agaaatcgat cgtggcgtcg agcgccacga gcgccgccgt gcgctgatct ccgatcacgc
       61 catgatcgcc aatccgcggg taccgctcgc cggcttcgac tccggccgcg cccggccccg
      121 actgcgagct cagaacccca gctccagcgt cgcgaacagg ctgcgcggct gaagcacgta
      181 atagtatggc cgatcgccgc tgtatccgct gccgtagcgc ttggagttgg tcaggttctc
      241 accgcgaagg gccaatgcgt gccgtccgtg agcgaaatgc cactcgaaga gcccgtccat
      301 cacgtacgac gacggcagca ccatgttcgg attgcccgtg ttgtcgagct gcgagcggcc
      361 ggtgtagcga ccacctaacg agatcgtgaa ccgccgcgtc ccctgccacg tcagccgctg
      421 cgccgtcgtg accttcggcg tcagcagcgg ctcgacatcg tgaaaggtct cgccgctcga
      481 gtcatcggtg tagtcggcga tcttcgcgtt catgatcgtc gcgttggctg acgccgagaa
      541 ccccgctggc agcatgtagc cggcgtcgag ctccacaccg cgccggtagc ttgcgcgcac
      601 gttcttccgc aacggaagtc cgaggtagct cagtgcgccg attggctcga tctcgttgcg
      661 gaaatccatc gagaagatgt tcccctcgaa tcgcagcgcg cccgtccgca cgttcacgcc
      721 gctctcgata tcgtgcacgg tctccggctt cacgcgcgag agcggaccca cgaaggccac
      781 gttcgatgtg tccaggttgt cgaagcccgc gagcatgtcg ttccgcgccg gctctctgct
      841 catcgtgccg tacgacgcgt acagggacaa cgacggcgcc agtcgatacg tcagccctcc
      901 acgcggattc acgaacgtcc acgagatgga ggccggcgcc acgtcggcgt gcacgtccgg
      961 cgtgtagcgc cactgcgcgc gccgcacctg gacgtccccg aacaacgtcg cgcggcccac
     1021 gtcgtacgcc ac
//
LOCUS       DASUCJ010001032         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1493478, whole genome shotgun sequence.
ACCESSION   DASUCJ010001032 DASUCJ010000000
VERSION     DASUCJ010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493478"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="acs"
                     /locus_tag="VFU90_12195"
     CDS             complement(<1..1408)
                     /gene="acs"
                     /locus_tag="VFU90_12195"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5480593.1"
                     /translation="MPMIPELPVAMLACARIGAAHSVIFGGFSPESIVDRVNDAQCTT
                     LITCDWGWRRGNKIALKRNCDVAMEQTPSIRHCVVARRIGDDVYMHEGRDYWWSEVVA
                     GQPATFEPEAMNAEDLLFLLYTSGTTAKPKGIKHTTGGYLTQCAMTHELVFDLKPDGD
                     VYWCTADIGWVTGHSYIVYGPLANGATGVLYEGTPDYPDKDRFWEIVERYAVSILYTA
                     PTAIRTFMKWGVEYPAKHDLSSLRLLGTVGEPINPEAWMWYREWIGGNRVPVVDTWWQ
                     TETGAIMIAPLPGITTLLPGSATRPLPGIAADIVNDRGETVPPGGGGYAVLTRPWPGM
                     LRGIWGDDARYEQTYWSKFPHLYLAGDGCRRDANGNYWFMGRIDDVMNVSGHRISTTE
                     VESALVDHPLVAEAAVCGKLDEMTGQAIYAFVTLRGGDPGSAELADELRGHVADKLGK
                     FTRPKYVTFTQELPKTRSG"
BASE COUNT          226 a          454 c          475 g          263 t
ORIGIN      
        1 ttccgctgcg cgttttcggc aattcttggg tgaatgtaac gtacttcggg cgggtgaact
       61 ttccgagttt atcggcgacg tgcccgcgca gctcgtcggc cagctcggcc gatccgggat
      121 cgccgccgcg cagcgtgacg aacgcataga tcgcctgacc ggtcatctcg tcgagcttgc
      181 cgcagacggc ggcctcggcg accagcggat ggtcgaccag cgcgctttcg acttcggtgg
      241 tggaaatacg gtggccgctg acgttcatca cgtcgtcgat ccggcccatg aaccagtagt
      301 tgccgttcgc atcgcgccgg cagccgtcgc cggcgagata gaggtgcgga aacttcgacc
      361 agtaggtctg ttcgtagcgt gcgtcgtcgc cccaaatccc gcgcagcatt cccggccacg
      421 ggcgcgtcag cacggcgtat ccgccgccgc ccggcggaac cgtttcgccg cggtcgttga
      481 cgatgtcggc ggcgatgccc ggaagcgggc gcgtcgcgct gcccgggagc agcgtcgtga
      541 tgcccggcag cggcgcgatc atgatggcgc cggtttcggt ttgccaccac gtgtcgacga
      601 ccggaacgcg gttgccgccg atccattccc gataccacat ccaagcttcg gggttgatcg
      661 gttcgccgac ggtgccgagc agccgcagcg acgagagatc gtgcttggcc ggatattcga
      721 cgccccactt catgaaggta cggattgcgg tcggcgcggt gtagaggatg ctgacggcgt
      781 accgctcgac gatctcccag aagcggtcct tgtcgggata atcgggcgtg ccttcgtaca
      841 gcacgccggt cgcgccgttg gcgagcgggc cgtagacgat gtacgagtgt ccggtcaccc
      901 aaccgatgtc ggcggtgcac cagtagacgt cgccgtcggg cttgagatcg aagacgagct
      961 cgtgcgtcat cgcgcactgc gtcaggtaac cgccggtggt gtgcttgatc cccttcggct
     1021 tggcggtcgt gccgctggtg tagagcagga agagcagatc ttcggcgttc atcgcctccg
     1081 gctcgaacgt cgccggctga ccggcgacga cctcgctcca ccagtagtcg cgcccttcgt
     1141 gcatgtagac gtcgtcgccg atgcgccggg ctacgacgca atgccggatc gacggcgtct
     1201 gctccatcgc gacgtcgcag ttgcgtttga gcgcgatctt gttgccgcgc cgccagcccc
     1261 agtcgcaggt aatcaaggtg gtgcactgcg cgtcgttgac gcgatcgacg atcgattccg
     1321 gcgagaaccc gccgaaaatc accgagtgtg cggcgccgat ccgcgcgcac gcgagcatcg
     1381 cgaccggaag ctcgggaatc atcggcatgt aaatcgca
//
LOCUS       DASUCJ010001033         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_285883, whole genome shotgun sequence.
ACCESSION   DASUCJ010001033 DASUCJ010000000
VERSION     DASUCJ010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285883"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFU90_12200"
     CDS             <1..273
                     /locus_tag="VFU90_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5480594.1"
                     /translation="AESSKPAPDILQAALAQSGLAAADVIFVGDSVWDVDAAGKLHIP
                     CIGLTSGGLAADELLRAGAVECYESPRDLHDQLPTSKVVQRLFERR"
     gene            355..651
                     /locus_tag="VFU90_12205"
     CDS             355..651
                     /locus_tag="VFU90_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480595.1"
                     /translation="MTNHRKIAVVDPTRERRPAGNTALIAAELSAVRCRWCNDDLEHC
                     HEALVRHAIGDVHCMSPECETPPELHHMIIDCSEFGCGCAESAALSGSLGNGAA"
     gene            862..>1165
                     /locus_tag="VFU90_12210"
     CDS             862..>1165
                     /locus_tag="VFU90_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480596.1"
                     /translation="MIVAPLAGSIAAMAVLSRALPTPSSGSAVILRLGWWAAILIGSL
                     CAMWLLERLTKRLAPLAALCDMAVLFPGRAPSRLAVARRAGDVRMLQSLVKSPDVPG"
BASE COUNT          188 a          388 c          393 g          196 t
ORIGIN      
        1 gccgaatcga gcaagccggc gcctgacatc ttgcaagcgg ccctcgccca atccgggcta
       61 gccgccgccg acgtgatctt cgtcggggat tcggtctggg acgtcgacgc cgccggcaag
      121 ctccacattc cgtgcatagg actcaccagc ggaggcttgg cagccgacga gctgctacgc
      181 gcgggcgcgg tggagtgtta tgaatccccc cgcgatcttc acgatcagct gccgacgagc
      241 aaggtcgttc aacggctgtt cgaacgacgt tgacatcggc cgttcggtcg aaagtccaac
      301 gcccaagcgc gtcgcagcca gtaaatgcgc gcgtcgcggc ttacgttcgt gagcatgacg
      361 aaccatcgca agatcgcggt cgtcgatccc acccgcgagc gccggccagc tggcaacacc
      421 gcgctcatcg ccgcggagct gtccgccgtc cgatgccgct ggtgcaatga cgatctcgag
      481 cattgtcacg aggcgctcgt gcggcatgcg atcggcgacg tccattgcat gtcgcccgag
      541 tgcgaaacac cgccggagct ccaccacatg atcattgact gcagcgaatt cggctgcggt
      601 tgcgcagaga gcgccgcgct atccggctcg ctcggaaacg gcgcagccta gtcgtcgcac
      661 gaggtagttg ccaccctccc tataaagcga cccagcacat cggccgatgc gtcgatggcg
      721 gaattggtgt cgtgaggggc ggcggtgacg agcggaacgc gtcggcgagg caatcggtcg
      781 cacggccctc gtcggaacgc aatcgtcgcg gaggaacgat ggcaacgccg acgcgcggcc
      841 gcgctcgcac tgcggacgac gatgatcgtc gcgccgctgg ctggctcgat cgccgcgatg
      901 gcggtcctca gccgggcact tccgacgcct tccagtggca gcgcggtcat cctccggctg
      961 ggctggtggg cggcgatctt gattggctcg ctgtgtgcga tgtggctgct tgagcggctg
     1021 acgaagcgcc ttgcaccgct cgccgcgctg tgcgacatgg cggttctctt tcccggccgc
     1081 gcgccgagcc gtctcgcggt cgcccgacgc gccggcgacg tgcggatgtt gcagtcgctg
     1141 gtcaagagcc cagatgtgcc gggcg
//
LOCUS       DASUCJ010001034         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_270815, whole genome shotgun sequence.
ACCESSION   DASUCJ010001034 DASUCJ010000000
VERSION     DASUCJ010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270815"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFU90_12215"
     CDS             <1..1032
                     /locus_tag="VFU90_12215"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF006589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU5480597.1"
                     /translation="LMSTLLSTTLAGSLPKPSWLAEPEALWAAWKLEGDALAEGKRDA
                     VRLALCMQERAGIDIVTDGEQTRRHFVTTFIEGLDGVDFEHRKTVHIRNRYDADVPVV
                     TGPVARRHPVFVDDAAFLRSQTTHRVKFTLPGPMTMVDTLFDAHYGSREKLAFAFAEI
                     LNEEAKDIAKAGIDIVQFDEPAFNVYFDEVRDWGIAALERAARGLGCKTAVHICYGYG
                     IKANIDWKKSLGAEWRQYEQTFPLLARSSIDQVSLECAHSHVPIDLIGLLPGKDVMVG
                     AIDVATSDVETPEDVAAVIRSALRFVPAERLLPCTNCGMVPLARPIAQAKLEALARGT
                     ALVRQEIAS"
     gene            1289..>1431
                     /locus_tag="VFU90_12220"
     CDS             1289..>1431
                     /locus_tag="VFU90_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5480598.1"
                     /translation="MAMATGTVKWFNDAKGFGFITPDGGGEDLFAHFSAINMQGFKTL
                     KEG"
BASE COUNT          266 a          475 c          440 g          250 t
ORIGIN      
        1 ctcatgagca cgctcctttc gacgaccctc gccggcagtc tcccgaaacc ctcgtggctc
       61 gcggagccgg aagcgctgtg ggccgcatgg aagctcgagg gcgacgccct tgccgaaggc
      121 aagcgcgacg cggtccggct ggcgctttgc atgcaggagc gcgccggcat cgatatcgtc
      181 accgacggcg agcagacgcg gcgtcacttc gtgaccacct tcatcgaggg gctcgacggg
      241 gtcgacttcg agcaccggaa gaccgtgcac atccgtaacc gctacgacgc cgacgtgccg
      301 gtggtgaccg gcccggtcgc gcgccgccac ccggtcttcg tcgacgatgc cgcgttcctg
      361 cgctcgcaga ccacgcaccg ggtcaagttc acgctgcccg gcccgatgac gatggtcgac
      421 acgctcttcg acgcgcacta cggcagccgc gagaagctcg cgttcgcctt cgccgagatc
      481 ctgaacgaag aggcgaagga catcgcgaaa gccggcatcg acatcgtgca gttcgacgag
      541 ccggcgttca acgtctactt cgacgaggtg cgcgactggg gcatcgccgc gcttgagcgg
      601 gcggcgcgcg ggctcggctg caagacggcc gtgcacatct gctacggcta cggcatcaag
      661 gccaacatcg actggaagaa gtcgctcggc gccgagtggc gccagtacga gcagaccttc
      721 cctctcctcg ccagatcttc gatcgaccag gtgtcgctcg agtgcgcgca ttcgcacgtc
      781 ccgatcgacc tcatcggcct gctgccgggc aaggacgtca tggtcggcgc gatcgacgtc
      841 gcgacgagcg acgtcgagac gcccgaagat gtcgccgcgg tgatccgctc cgcactgcga
      901 ttcgtcccgg cggaacgttt gctgccgtgc acaaactgcg gcatggttcc gcttgcccgg
      961 ccgattgcgc aagccaagct cgaagccctc gcccgtggca ccgcattggt gcggcaagaa
     1021 atcgcttcct gaccgtcggt catttatttc gcgcgaatcg gccgaacccg gcaccgggcg
     1081 tgggtttccg cgcaacactc cgacccaccc ccgcgcgaaa cctatcgatt tattgcagga
     1141 aatcccgtat agtgcgcgcc gtgttttgtt ccaagcagta aagtgcttgc tctgtacgac
     1201 cgccacgcag tcgttcgatc gccgtacttt ccttgagatc gaaacatcgt ccgggcaacg
     1261 tcccgtttaa ctcgaaattg aggaatcaat agcaatggca accggaactg taaagtggtt
     1321 caacgacgcg aagggcttcg ggttcatcac gccggatggc ggcggtgaag acctgttcgc
     1381 tcacttctcg gcgatcaaca tgcagggctt caagacgctc aaggaaggcc a
//
LOCUS       DASUCJ010001035         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_331554, whole genome shotgun sequence.
ACCESSION   DASUCJ010001035 DASUCJ010000000
VERSION     DASUCJ010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331554"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFU90_12225"
     CDS             complement(<1..218)
                     /locus_tag="VFU90_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480599.1"
                     /translation="MTDAQSSDTQRLSDDALLDRVQRQTLKYFWEFAHPASGMARERS
                     NPVAGYDYLETVCSGGTGFGIMALIAGAA"
     gene            complement(215..>1029)
                     /locus_tag="VFU90_12230"
     CDS             complement(215..>1029)
                     /locus_tag="VFU90_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEU5480600.1"
                     /translation="ATRTEIGLPGSQRQLLQALHATGKPLVIVVMSGRPLALVWEDQH
                     ADAILSAWFAGSEAGNGIADVLFGDVNPSGKLAMSLPRSVGQCPIYYAEPPTGRPPGM
                     IGIDAEGDNERDARGRHVFRKFTTACRVDGPSTPLYPFGYGLSYSTFDYGTISVDKTM
                     LRGDDLLEASIEVRNSSLLAGEEIVQLYIGDPVASRSRPVRELKDFQKILLQPGEQRV
                     VRFTVTTADLTFFHADRLAEPEALWEPGEFVIEIGRNSDELSAISISWQADS"
BASE COUNT          179 a          329 c          335 g          186 t
ORIGIN      
        1 gcggcgccag cgatgagcgc catgataccg aagcccgtgc cgccgctgca gaccgtctcg
       61 aggtagtcgt agcccgccac cggattgctg cgctcgcgcg ccattccgct cgccggatgc
      121 gcgaactccc agaaatattt cagcgtctgc cgctgcacgc gatcgagcag ggcgtcgtcg
      181 gaaaggcgtt gcgtgtctga ggattgcgcg tccgtcaaga atcggcctgc catgagatgc
      241 tgatcgccga aagctcgtcc gagttgcggc cgatctcgat gacgaattcg cccggctccc
      301 acagggcttc cggctctgcg agacgatcgg cgtggaagaa agttaggtcg gcggtcgtga
      361 cggtaaaccg cacgacgcgt tgctcgccgg gctgcaacag tatcttctgg aagtctttca
      421 gttcccgtac cggacgcgag cggctggcga ccggatcgcc aatgtacaac tgcacgatct
      481 cctcgccggc cagcaaactg ctgttgcgaa cctcgatcga agcctcgagc aggtcgtcgc
      541 cgcgcagcat ggtcttgtcg acactgatcg tgccgtagtc gaacgtgctg tagctcaggc
      601 cgtagccgaa cggatagagc ggcgtggacg gcccgtcgac gcggcaagcc gtcgtgaact
      661 tgcggaacac atgccgcccg cgggcgtcgc gttcattgtc gccttcagcg tcgatgccga
      721 tcatgccggg gggacggccg gtcggcggct cggcgtaata gattgggcat tgaccaaccg
      781 agcgcggcaa gctcatcgcc agtttgccgg acgggttgac gtcgccgaac agtacatcgg
      841 cgatgccgtt gccggcctcg ctgccggcaa accatgcgct gaggatggcg tccgcgtgct
      901 ggtcctccca caccagcgcc agcggccgtc cgctcatgac gacgatcacc agaggttttc
      961 cggtagcatg caacgcctgc aacagttgcc gttgcgagcc gggcaatccg atctcggtgc
     1021 gtgtggccg
//
LOCUS       DASUCJ010001036         1260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_224952, whole genome shotgun sequence.
ACCESSION   DASUCJ010001036 DASUCJ010000000
VERSION     DASUCJ010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224952"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFU90_12235"
     CDS             <1..804
                     /locus_tag="VFU90_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5480601.1"
                     /translation="NVYEPHEMPWPELDETSLARLRAIPVWSMALEIETSAGSMLDGF
                     AKTEPDPLVRRALELQGFEESRHGRILAEMIARYGLTAKPKETDEQPTRRAFINFGYD
                     ECVDSFAGFGIFKLARDARILPDSLTSLFTRVLEEEARHIVFFVNWIAWDRNRRGLRG
                     PAMQAIPALAGYAAAIARRVQGGSQMAGSGSSGGNSERLDLFADVMRDLTPAKFIRAC
                     VDENDRYMRVFDPRLLRPRVIPTLGRIALAIAEAIDRIRAVLNPGEARA"
     gene            815..>1260
                     /locus_tag="VFU90_12240"
     CDS             815..>1260
                     /locus_tag="VFU90_12240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5480602.1"
                     /translation="MNPLPSGTITFAFTDIEASSARWERDRAAMQAAVRRHDALVRAA
                     IVQNTGSVFKTIGDAFCAAFSRPGDAVAAMLEVQRSLLGEDFSALGGLFVRAAIHTGT
                     ADERDGDYFGPAVNRVARLLAIGHGGQVLVSGATAAVLQGDLPDLA"
BASE COUNT          207 a          406 c          426 g          221 t
ORIGIN      
        1 aacgtctacg aaccgcacga aatgccgtgg cccgagttgg acgaaacgtc gctcgcccgc
       61 ctgcgcgcca ttccggtctg gagtatggcg ctcgagatcg agaccagcgc gggctccatg
      121 ctcgacggat ttgccaagac cgagccggat ccgctcgtgc gccgcgcgct cgagctccag
      181 ggcttcgaag agtcgcggca cggacgcatc ctggcggaga tgatcgcgcg ttacgggctg
      241 acggcgaagc ccaaggaaac cgacgaacag ccgacgcgcc gggcattcat caacttcggc
      301 tacgacgagt gcgtcgactc gttcgcgggc tttggaatat tcaagctggc ccgcgacgcc
      361 cgcatcttgc ccgattctct gacgtcgctg tttacgcgcg tcctcgaaga ggaagcgcgg
      421 cacatcgtgt ttttcgtcaa ctggattgcc tgggatcgca accggcgggg tcttcgcggc
      481 cccgcgatgc aggcgattcc ggcgctggca gggtacgccg ccgcgatcgc gcgtcgcgtc
      541 cagggcggca gccagatggc cggaagtgga agcagcggcg gcaacagcga gcgtctggat
      601 ctcttcgccg acgtcatgcg agatctcacg ccggccaaat ttatacgcgc gtgcgtggac
      661 gagaacgatc gctatatgcg tgtcttcgat ccgcgcctgt tgcggccgcg cgtgatcccg
      721 acgctcggcc gaatcgctct ggcgatcgcc gaagcgatcg accggattcg ggcggtactg
      781 aacccgggtg aggcgcgcgc ctaaggaacc tgccgtgaac cctttgccga gcgggaccat
      841 aacgttcgcc ttcaccgaca tcgaggccag ttccgcgcgc tgggaacgcg accgggcggc
      901 gatgcaggcg gcggtccgcc gtcacgatgc gctagtgcgt gcggcgatcg tgcaaaatac
      961 aggatccgtt ttcaagacga ttggggacgc gttctgcgcc gcgttttcgc gccccggcga
     1021 tgcggtcgcc gcaatgcttg aggtccagcg ctcgctgctc ggcgaggact tttcggcact
     1081 aggcggcctt ttcgtccgcg cggcgattca taccggcacc gccgacgagc gcgacggaga
     1141 ctacttcgga ccggccgtta atcgcgtcgc gcgcctattg gcgattgggc atggcggcca
     1201 agtgctcgtc tccggcgcga cggcggccgt gctgcagggc gatctgccgg atctcgcgag
//
LOCUS       DASUCJ010001037         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_41416, whole genome shotgun sequence.
ACCESSION   DASUCJ010001037 DASUCJ010000000
VERSION     DASUCJ010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41416"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFU90_12245"
     CDS             complement(<1..499)
                     /locus_tag="VFU90_12245"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480603.1"
                     /translation="MKLALIGDPVEHSRSPQLHRLFLEEAEIDGSYVAIAVPAGRAGA
                     AIRRLRDEGYLGCNVTYPLKEEALAACDRLTTEARRSDAVNTIFFGREIEGTDTDGAG
                     ARTAIESLIEEPIALKRIGVLGYGATARAILAELHDNDAYAFVWGRDQERVRAVCERY
                     EASPWP"
     gene            complement(496..981)
                     /locus_tag="VFU90_12250"
     CDS             complement(496..981)
                     /locus_tag="VFU90_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU5480604.1"
                     /translation="MLGPNRYAPRLHDVPHEELEAAGIRGLIVDLDNTLVGYRETELA
                     REHIEWVARAHDRGFRIVMLSNNFSGRVTSIAARLNVACIPNALKPLPFGFLRAVRNL
                     DMRRREIAVVGDQLFTDVLGGKLCGLYTILTEPIETKDLGLTSFFRFFERLVLPSRRP
                     R"
BASE COUNT          182 a          384 c          342 g          187 t
ORIGIN      
        1 cgggccacgg actcgcttcg tagcgttcgc acaccgcgcg aacgcgctct tgatcgcgcc
       61 cccaaacgaa cgcgtacgcg tcgttgtcgt gaagctcggc gagaatggcg cgggcggtgg
      121 cgccgtatcc gagaacgccg atacgtttga gtgcgatcgg ctcctcgatg agcgactcga
      181 tcgccgtccg cgcaccggcg ccgtcggtgt ccgttccctc gatctcgcgg ccgaagaaaa
      241 tcgtgttgac cgcatccgaa cgccgcgctt cggtggtgag ccggtcgcag gccgccagcg
      301 cttcttcctt cagcggatag gtgacgttgc agccgaggta cccctcatcg cgcaaccggc
      361 ggatcgccgc cccggcgcgt ccggccggca ccgcgatcgc cacgtagctg ccgtcgattt
      421 ccgcttcctc caagaaaagg cggtgcagct gcgggctgcg gctgtgttcg accgggtcgc
      481 cgatcagcgc gagcttcatc gcggccgccg gctgggcagc acgagccgct cgaaaaaccg
      541 gaagaacgac gtcaggccta gatctttcgt ttcgatcggc tcggtgagaa tcgtgtacag
      601 cccgcacagc ttcccgccca gcacgtcggt aaacagctga tcgccgacga cggcgatttc
      661 gcgccgccgc atgtcgaggt tgcgcaccgc acgcaaaaag ccgaacggca acggtttgag
      721 cgcgttcgga atgcaggcca cgttgagccg cgcggcaatc gacgttaccc gccccgaaaa
      781 gttattcgaa agcatcacga tccgaaagcc gcgatcgtgc gcccgggcga cccactcgat
      841 gtgctcgcgc gccagctcgg tttcgcggta gccgaccagg gtgttgtcga ggtcgacgat
      901 cagcccccga atgcccgccg cctccagctc ctcgtggggc acgtcgtgga gtcgcggggc
      961 gtaccgatta ggcccgagca tgcgccctcc gttgcaccgg ttatcccccg tatcctcgaa
     1021 gatctccaca ccggctccac gcggccgccg gccactcgca aactgtccgc cggttttccc
     1081 ggctgctcac aggtt
//
LOCUS       DASUCJ010001038         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1401892, whole genome shotgun sequence.
ACCESSION   DASUCJ010001038 DASUCJ010000000
VERSION     DASUCJ010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401892"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VFU90_12255"
     CDS             complement(<1..955)
                     /locus_tag="VFU90_12255"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF006439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEU5480605.1"
                     /translation="MTALLAATLRHLRALVAFDTRNPPRAIGADGIFDYLRANLPDFD
                     CTLTDHGAGAVSLFAVRGKPKVLFNVHLDTVPDSPYWSASPFELRKTDERAVGLGACD
                     IKGAAAALLAAAQASDGDAAYLFTTDEEANDARCIAAFLTQKKPFDAVIVAEPTRCEA
                     VLAHRGISSVLMRFAGRAGHAAGEQTAGDSALHQAVRWSSRALDHVDSLAHVRFGGLT
                     GLRFNIGRIDGGIKANMIAPAAELRFGFRPLPSMDVDGLLATFRGFAEIEPAEFGETF
                     RGPALPADADFDDNGAGAEARRLAARDVADALDLPVGNAVDF"
     gene            complement(952..>1307)
                     /locus_tag="VFU90_12260"
     CDS             complement(952..>1307)
                     /locus_tag="VFU90_12260"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480606.1"
                     /translation="LVAHRALEEAVLTKQQNRFKPDVARKWVELVYEGFFHDPLKTDL
                     EAFLASSQRRVAGDVVLETHGGGVVAVAVESPHILNAKDATYAQSADWGVVEAEGFIK
                     LFGMSSTMWAQVNRK"
BASE COUNT          232 a          438 c          426 g          211 t
ORIGIN      
        1 agaaatcgac cgcattgccg acgggaagat cgagcgcgtc ggcgacgtcg cgcgcagcca
       61 agcggcgcgc ctcggcccct gcaccgttat cgtcaaaatc agcgtccgcc ggcaacgccg
      121 ggccgcgaaa cgtttcgccg aactcggcag gctcgatttc ggcaaagccg cggaaggtcg
      181 cgagcaggcc atcgacgtcc atcgacggca acggccggaa gccgaaacgc agctccgccg
      241 ccggcgcgat catgttcgcc ttgatgccgc cgtcgatgcg gccgatgttg aagcgcagtc
      301 ccgtcaggcc gccgaaacgc acgtgtgcga gcgaatcgac gtgatcgagc gcgcggctgc
      361 tccagcgcac ggcctgatgc aacgcactgt cgcccgccgt ctgttcgccg gcagcgtggc
      421 cggcgcgccc ggcaaaacgc atcagcaccg agctgatgcc acgatgtgcg agaacggctt
      481 cacaccgcgt cggctcggcc acgatcacgg cgtcaaaggg ttttttttgc gtgaggaacg
      541 ccgcgatgca gcgcgcgtcg ttcgcctcct cgtcggtggt gaacaggtag gcggcatcgc
      601 cgtcgctggc ttgcgcggcg gcgagcagcg cagcggcggc gcccttgatg tcgcaggcgc
      661 cgaggccgac ggcgcgttcg tccgtttttc gcaattcgaa cggactcgcg ctccaatacg
      721 gcgaatccgg caccgtgtcg aggtgcacgt tgaacaggac cttcggcttg ccgcgcacgg
      781 cgaacagcga caccgcgccg gcgccatggt cggtcagcgt gcagtcgaaa tccggcaggt
      841 ttgcgcgcag gtaatcgaaa atgccgtccg caccaatcgc acgcggtgga ttgcgcgtgt
      901 cgaaggcgac cagcgcccgc agatgccgca gcgtcgcggc gagaagcgcc gtcatttccg
      961 gttcacctgt gcccacatcg tcgaactcat gccgaacagc ttgatgaagc cttcggcctc
     1021 gaccacaccc caatccgccg actgcgcgta ggtcgcgtct ttcgcattga ggatatgcgg
     1081 cgattcgacc gcgacggcaa cgacgccgcc accgtgggtc tcgagcacga catcgcccgc
     1141 aaccctgcgc tggctcgatg cgagaaaggc ctcgagatcg gtcttgagcg gatcgtggaa
     1201 gaatccttcg tagacgagtt ccacccactt gcgagccacg tcgggcttga agcggttttg
     1261 ctgcttggtc agcacggctt cttccagcgc gcggtgcgcg acgagca
//
LOCUS       DASUCJ010001039         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_484836, whole genome shotgun sequence.
ACCESSION   DASUCJ010001039 DASUCJ010000000
VERSION     DASUCJ010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484836"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFU90_12265"
     CDS             complement(<1..312)
                     /locus_tag="VFU90_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480607.1"
                     /translation="MSTVESDVMVIGAGAHGSAAAYALARRGVKVTAIDRWAPPHAHG
                     SSHGRTRIIREAYFEHPVYVPLVRRAYHLWAELERETGRALFRRTGGLTLGAEDGVLV
                     SG"
     gene            complement(309..>1072)
                     /locus_tag="VFU90_12270"
     CDS             complement(309..>1072)
                     /locus_tag="VFU90_12270"
                     /inference="COORDINATES: protein motif:HMM:NF018067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate dehydrogenase
                     kinase/phosphatase-domain containing protein"
                     /protein_id="HEU5480608.1"
                     /translation="GLVMSVFTLPSLNVVFKIIKDRFGHPKRTTRRRVMDKYQFVFVR
                     DRVGRLADAQEFEHLDLPRACFSEPLLEHLLDAAASTVELRGDHVIVRHLYTERRVTP
                     LNVYLRDASPYDAALAVLDYGTAIKDLAAANIFTGDMLMKNFGVSRHGRVIFYDYDEL
                     SLLTDCVFRRLPQPQTPEQEMAAEPWYYVGENDVFPEEFEAFMVPPGKLRKRFLQRHG
                     DLFTVEFWQSMQERQRHGDMPDVFPYPESRRLRHT"
BASE COUNT          181 a          370 c          347 g          174 t
ORIGIN      
        1 gcccgacacg agcacgccat cttcggcgcc taacgtcagg cccccggtgc ggcggaagag
       61 cgcgcggccg gtctcgcgct cgagctcggc ccagagatgg taggcgcggc gcacgagcgg
      121 gacgtacacc ggatgctcga agtacgcttc acggatgatg cgcgtccgtc cgtgcgacga
      181 cccgtgcgcg tgcggcggtg cccaccggtc gatcgccgtc accttcacgc cgcgccgcgc
      241 cagcgcgtac gccgccgcgc tgccgtgagc cccggcgccg atcaccatca cgtcactctc
      301 gacggtgctc acgtgtgccg gagccggcgc gactcgggat aggggaagac gtcgggcatg
      361 tcgccgtggc gctgccgctc ctgcatcgac tgccagaatt ccaccgtgaa caggtcgccg
      421 tgccgctgca ggaaccgctt gcggagtttg cccggcggca ccatgaacgc ctcgaattcc
      481 tccgggaaca cgtcgttctc gcccacgtag taccacggct cggcggccat ttcctgctcc
      541 ggcgtttgcg gttgcggcag ccggcggaac acgcaatcgg tgagcagcga caattcgtcg
      601 tagtcgtaga agatcacgcg accgtggcgg ctcacgccga agttcttcat caacatgtcg
      661 ccggtgaaga tgttcgctgc cgccagatcc ttgatggccg tgccgtagtc gagcacggcc
      721 agcgccgcgt cgtagggcga cgcatcgcgc agatacacgt tgagcggcgt cacgcgccgc
      781 tccgtgtata gatgcctaac gatgacgtgg tcgccccgga gctcaacggt ggacgccgcc
      841 gcgtccaaca gatgctcgag caacggctcg ctgaagcacg cgcgcggcag gtcgaggtgt
      901 tcgaactctt gcgcgtcggc cagacgcccc acgcggtcgc gcacgaacac gaactggtat
      961 ttgtccatca cccggcgacg cgtggtccgc ttgggatgcc cgaagcgatc cttgatgatc
     1021 ttgaacacca cattgagcga cggcagcgtg aacacgctca tcacgagccc ct
//
LOCUS       DASUCJ010001040         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_882420, whole genome shotgun sequence.
ACCESSION   DASUCJ010001040 DASUCJ010000000
VERSION     DASUCJ010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882420"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFU90_12275"
     CDS             complement(<1..384)
                     /locus_tag="VFU90_12275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5480609.1"
                     /translation="MTTPDYRRISFWFDSFAAAGGDLTPRATLRGDIAVDIAIVGAGF
                     TGLWTAYYLQRLDQSLRIAVIEKEIAGFGASGRNGGWCSALFPSSDEKVAKLSSRDDA
                     LALRAAMRATVDEVGAVAAQEGIDCD"
     gene            complement(381..1055)
                     /locus_tag="VFU90_12280"
     CDS             complement(381..1055)
                     /locus_tag="VFU90_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5480610.1"
                     /translation="MGDSLSSEAGSEASSARTRVRRLPEKQVFSRAALNAILDAGRVG
                     HLATSDEQGQPYVLPVAYARDDDHIIVHGSTGSRLFRSLAAGSATCFTVTLLDGLVLA
                     RSAFESSMRYRSAMVFGSCTAISDDDTLRAFEVLTEHLMPRRWADLRPPLRKEIAATA
                     MLALPIDEWSVKVSDGWPEDEPDDVELPIWAGVLPIQHSFGAPIVAPDVPTDVRPPEY
                     LRHWSA"
     gene            complement(1040..>1249)
                     /locus_tag="VFU90_12285"
     CDS             complement(1040..>1249)
                     /locus_tag="VFU90_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480611.1"
                     /translation="RETFDDDESERLLRGYLSGALFDAGLICRADDRGDPVIQLAPPL
                     IVEQEEFDFIEQTLRSVLTKVWEIL"
BASE COUNT          226 a          423 c          395 g          205 t
ORIGIN      
        1 atcgcaatcg attccttcct gcgcggctac cgcgccgacc tcgtcgacgg tcgcgcgcat
       61 ggccgcgcgc aacgcgagcg cgtcgtcgcg cgatgacagc ttcgccacct tttcatcgga
      121 cgacgggaac aacgcgctgc accagccgcc gttgcggccg gatgcgccga agccggcgat
      181 ctctttctct ataacggcga ttcgaaggct ttggtcgaga cgttgcaggt agtacgcggt
      241 ccacaacccg gtgaagccag ccccgacgat cgcgatgtcg accgcgatgt ctccccgcag
      301 cgtcgcgcgt ggcgtgaggt cgccgcccgc ggcggcgaac gagtcgaacc aaaagctgat
      361 tcgccgatag tcgggcgtcg tcatgccgac cagtgccgta ggtactcagg cggccgcaca
      421 tcagtcggga catctggcgc gactatcggc gcgccgaaac tgtgctgaat cggcagcacg
      481 cccgcccaga tcggcagctc aacgtcgtcg ggctcgtctt cgggccagcc gtcgctgacc
      541 ttcaccgacc actcgtcaat cgggagcgca agcatcgccg ttgccgcgat ctccttgcgc
      601 agcggcggcc tcaggtcagc ccatcggcgt ggcatcagat gctcggtcaa cacctcaaac
      661 gcgcgcagcg tgtcgtcgtc cgatatcgct gtgcatgaac cgaagaccat tgcgctgcgg
      721 tagcgcatcg aggattcaaa cgccgaccgt gcgagcacca agccgtcgag caatgtgacg
      781 gtgaagcagg tagccgaacc agcggctaac gatcgaaaca gccggctgcc cgtcgatccg
      841 tggacgatga tgtggtcgtc atcgcgcgcg tacgccaccg gcagcacata cggctgccct
      901 tgttcatcgg acgtcgcgag atggccgacc cggccggcgt caagaatcgc gttgagcgcg
      961 gcacgcgaga agacctgctt ctcaggtagg cgacgcaccc tcgttcgggc ggacgacgcc
     1021 tcggatcccg cctcgctgct cagagaatct cccacacctt ggtgagtacc gagcgcagcg
     1081 tctgctcgat gaagtcgaac tcctcctgct cgacgatgag cggcggcgcc agctggatta
     1141 ccgggtcgcc gcggtcgtcg gcgcgacaaa tgaggccggc gtcgaacagc gcgccggaca
     1201 ggtaaccgcg caatagccgt tcggactcgt cgtcgtcgaa cgtctcgcg
//
LOCUS       DASUCJ010001041         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_393019, whole genome shotgun sequence.
ACCESSION   DASUCJ010001041 DASUCJ010000000
VERSION     DASUCJ010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393019"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1255
                     /locus_tag="VFU90_12290"
     CDS             24..>1255
                     /locus_tag="VFU90_12290"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEU5480612.1"
                     /translation="MTIDRLLCPLRRAFVALAVLSVGLPSTSLSQVTGRKPPLRVPKE
                     PARLPVPLGPAKYVKEAGTMWTFDAPPLEYWKRTYGFTPDSAWLTHVRLASVRLPGCS
                     ASFVSARGLVLTNHHCARACVASASPADTNYNTTGFVAATIADERKCSTLYVDQLQSI
                     EDVTKRIRGAVTAKSADAQVQQRDSAIAQVQDECAASTHLRCEVVTLYQGGMYSLYRY
                     QRYTDVRLVMVPEEEAAFFGGDPDNFTYPRYDLDMALLRVYQNDSPMVVTDYLKWSKS
                     GAREGDLVFVTGNPGSTGRLLTLAQMQYLRDVQYPFTLAAYDRVIAMLNTLSAQSAGN
                     QRKYENLIFNIENSKKAITGYRAGLLDSTIMARKAAFERDLRARVDADPALRPKYGTA
                     WTDIANAERQLTAMAAQT"
BASE COUNT          226 a          391 c          415 g          223 t
ORIGIN      
        1 catttcgact cgacgaggct tacgtgacta tcgatcgcct gctctgtccg ctccgtcgcg
       61 cctttgtggc actcgccgtt ctttccgttg ggcttccatc gacatcattg tcgcaagtaa
      121 cggggcgcaa gccgccgctg cgcgtgccga aagagccggc gcggctgccg gttccgctcg
      181 gtccggcgaa gtacgtgaaa gaggcgggca cgatgtggac gttcgatgct ccgccgctcg
      241 agtactggaa gcggacgtac ggattcacgc cggactcggc atggctaacg cacgtgcggt
      301 tggcgtcggt gcggctgccg ggatgttcgg cgtcgttcgt ctcggcgcgc gggttggtcc
      361 tgaccaacca tcactgtgcg cgtgcgtgcg tcgcctcggc gtcgccggcg gacacgaact
      421 acaataccac cggcttcgtg gccgcgacga tcgcggacga acgaaagtgc tcgacgctgt
      481 acgtggacca actgcagtcg atcgaggatg tgacgaagcg cattcgcggc gccgtgaccg
      541 cgaagagcgc ggacgcgcag gtgcagcagc gcgattcggc aatcgcccag gtgcaggatg
      601 agtgcgcggc gtcgacgcac ctgcgctgtg aggtcgtgac gctctatcag ggcgggatgt
      661 actcgctgta ccgctatcaa cgctacaccg acgtcaggct cgtgatggtg ccggaagaag
      721 aggcggcgtt cttcggcggc gatccggaca acttcacgta tcctcgctac gacctcgaca
      781 tggccctcct gcgcgtctac cagaacgact cgccgatggt cgtgaccgat tatctcaagt
      841 ggagtaagtc cggcgcgcgc gagggcgatc tggttttcgt gacggggaat cccggctcga
      901 cgggccggct gctgacgctg gcgcagatgc agtacctgcg cgacgtacag tatccgttca
      961 cgctcgccgc ctacgatcgt gtcatcgcga tgctcaacac gttgagcgca cagagcgccg
     1021 ggaaccagcg caagtacgag aacctgatct tcaacatcga gaactcgaag aaggccatta
     1081 ccggataccg cgccgggttg ctcgactcga cgatcatggc gcggaaggcg gcgttcgagc
     1141 gggatcttcg tgcgcgcgtc gatgcagacc cggcgctccg gccgaagtac ggcaccgcct
     1201 ggaccgacat tgcgaacgcc gagcgacagc tcacggcaat ggccgcgcaa acgaa
//
LOCUS       DASUCJ010001042         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1478258, whole genome shotgun sequence.
ACCESSION   DASUCJ010001042 DASUCJ010000000
VERSION     DASUCJ010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478258"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2354
                     /locus_tag="VFU90_12295"
     CDS             <1..2354
                     /locus_tag="VFU90_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5480613.1"
                     /translation="LPELASLRDLGEHRLKDLERAERVYQLEAPDLPAAFPPLRSLNS
                     LATNLPVQLTSFLGREREIAEIAELVRERRLVTLVGSGGVGKTRAALQVAANLLDAFI
                     DGVWLIELEALSSGDFIASTVAHVLGIGLAGSADPTESLIQGLRQKSALLVFDNCEHL
                     AAPAARAIGAILSACPDVKVLASSRQPLAIDGEETYVLPPLEIPAAAALFAQRARAAD
                     KRFALTGENAATVAEICRRLDGVPLAIELAAPKITLLSLNQLLEMLHERFRLLAQPGV
                     DRLPRQQTLRALIDWSYDLLDDDERAAFARLSVFSGGWTLKAAAAVCTGGGIDEWRVF
                     ELLEALVSKSLVVTDSGDDERRFHMLNSIHEYSRQKLTASGETEAVAARHVSYYRDLV
                     RDLAPLLESLEDVQWQRALSPEIDNLRAAVDWAIFGRHDVAAGLQLLAQVEWAELLTT
                     PQEAIRWFDAAVALLDEPVDAVTKARVARHHVRLGWLVGRQIAQLEAFAVEALTVARA
                     AGDPSEVAQALANLAGVHRDAGRFERAEALYAEAYAEADALSRVATNAVLRNWAVSNL
                     QRGDTEMARQRFSEVARRERPGSEAHASALINIGELEFAVGNVEAARAAARAARETLV
                     HLRAAPLALVVCNLAAYAMAVDELDEARDLLREALDLLRESGARWMTTALEHHAMLAG
                     LIGDHERAAILVGFTNARYAGNDTRQTTERYGYERLMGLLTRAYDETELARQMSAGAS
                     LNAEQALEQAAAITQHHDRRRRPPSQRSTNHGQRDARTGSRKT"
     gene            2326..2499
                     /locus_tag="VFU90_12300"
     CDS             2326..2499
                     /locus_tag="VFU90_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480614.1"
                     /translation="MHEQAAGKLDAHGRAIDELHQHLAATPGVDKARLQTAVDKYKAA
                     YKQFRDDAQGCMN"
     gene            complement(2506..4011)
                     /locus_tag="VFU90_12305"
     CDS             complement(2506..4011)
                     /locus_tag="VFU90_12305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEU5480615.1"
                     /translation="MSGERRIPIPGTARQVWPGSRHADALGADAEADLTVWLRAGRGG
                     GVDLEVARATGTVSPRERRYASREALAHATGADPADVDAVRRYCDRFGLEVAGVHWRS
                     VTVAGPIDRLIRAFGATVAIFEDGLGRRFRHRSGTLHVLPELDGVVRGIFGLHQWPRS
                     KRVRSLERHATPLMAGEVASRYQFPESAGSGQTIGIVQLRGGFNGEDFDRCMAAQGVS
                     AQHPFVKPVDRTMVAHAAVTAKDVEAALDVQVVASLAPQARVVVYDAPDSERGFLDAL
                     REAVFGDEYAPNVVSVSYGWPENVWTPVALDIADELFAAAALLGISVACSSGDDGAAL
                     DIHGSPHVLAPASSPFAVACGATAILPAGEVAWQGSGGGFSARFPVPDWQATGADCAA
                     HYGMPAGRGVPDVAAQQSPGYYVVVDGTELAMGGTSAVAPVWAALVARINQRLGVPLG
                     FFTPILYRQAGTLFGAVTQGNNGRFDAGPGWNPCTGLGIPNGVAIEAALRS"
     gene            complement(4023..4304)
                     /locus_tag="VFU90_12310"
     CDS             complement(4023..4304)
                     /locus_tag="VFU90_12310"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU5480616.1"
                     /translation="MALKRYIIERDIPGAGNLSQDELKAVAQKSCSVLDGMGSDIRWV
                     HSYVTGDRVYCFYEAADEERIRQHAREGGFPANRISEVAAIIDPSTASG"
     gene            complement(4326..4658)
                     /locus_tag="VFU90_12315"
     CDS             complement(4326..4658)
                     /locus_tag="VFU90_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480617.1"
                     /translation="MRTIALLVLAVAVPLTAAAAALAPSAIVASPSTYDGKSVTVSGK
                     VTNFQSSHTMMGQVAGFQLCDSKCVVVIDETNQSRTNGASATVTGTFHSTFKGPRKTF
                     SNAVVISR"
BASE COUNT          840 a         1621 c         1556 g          782 t
ORIGIN      
        1 atctgccgga gctcgcgagc ctgcgcgacc tcggcgagca ccggctcaaa gatctcgagc
       61 gtgcggagcg cgtctatcag ttggaggcgc cggacctgcc cgccgccttc ccgccgctgc
      121 gctcgttgaa ctcgctcgcg acgaatctac cggttcagtt gacctccttc ctcggccgcg
      181 aacgcgagat cgccgaaatc gccgaactcg ttcgcgagcg gcgcctcgtg acgctggtcg
      241 gatcgggagg cgtcggcaag acgcgcgcgg ccttgcaagt ggcggccaac ctgctcgatg
      301 ctttcatcga cggcgtttgg ctgatcgaac tggaagcgct gtccagcggc gacttcattg
      361 catctacggt ggcgcacgtg ctgggcatcg ggctggccgg gagcgccgac ccgaccgaaa
      421 gcctcatcca ggggcttcgc caaaaaagcg ccttgctggt cttcgacaac tgcgagcatc
      481 tcgccgcgcc ggccgcccgc gcgatcggcg ccattttgag cgcctgcccg gacgtcaaag
      541 tgctcgcctc cagccggcaa ccgctggcga tcgacggcga agagacgtac gtcctgccgc
      601 cgctcgagat tccggccgcc gccgcactgt tcgctcagcg tgcgcgcgcc gccgataaac
      661 gctttgcgct gacgggcgag aacgctgcaa ccgttgcaga aatctgccgc cgcctcgacg
      721 gggtcccgct cgccatcgaa ttggcggctc cgaaaatcac gctactgagc ctcaatcagc
      781 tcttggagat gctgcacgaa cgcttccggt tgctcgcgca gccgggcgtc gatcgtctgc
      841 cgcgccagca gacgctgcgc gcgctgatcg attggagcta cgatctcctc gacgacgacg
      901 agcgcgcggc ctttgctcgg ctttccgtat tttccggcgg atggacgctg aaggctgccg
      961 ccgcggtctg caccggcggc ggcatcgatg agtggcgcgt tttcgaactg ctggaggcgc
     1021 tggtctcgaa gtccctggtg gtcacggatt ccggcgacga cgaacgccgc ttccatatgc
     1081 ttaactcgat ccacgaatac agccgtcaga agctgaccgc gtccggcgaa accgaagcgg
     1141 ttgccgcaag acacgttagc tactatcgcg atctcgttcg cgatctcgct ccgcttctgg
     1201 aatcgctgga agacgtccag tggcagcgcg ccctctcacc cgaaatcgac aatttacggg
     1261 cggccgtcga ctgggcgata ttcggccgtc acgacgtggc cgccgggttg caattgctgg
     1321 cccaggtcga gtgggcggag cttctcacca ccccgcagga ggcgattcgc tggttcgatg
     1381 cggccgttgc gctgctcgac gagccggtag acgcggtgac gaaagcgcgc gtcgcgcgcc
     1441 accacgtgcg gctcggatgg ctggtgggcc ggcaaatcgc gcaactcgaa gcgtttgccg
     1501 tcgaggcgct gaccgtcgcg cgcgctgccg gagatccgag cgaagtcgct caggcgctcg
     1561 ccaatcttgc gggcgtccat cgcgatgcgg ggcgcttcga gcgagccgaa gcgctttacg
     1621 ccgaagcgta cgccgaggcc gatgcacttt cgcgagtcgc gacgaacgcc gttttacgca
     1681 actgggccgt ctcgaatctt cagcgcggcg acaccgaaat ggcgcggcag cgctttagcg
     1741 aggtcgcgcg acgcgagcgg cccggcagcg aagcccatgc gagcgcgctc atcaacatcg
     1801 gtgaactcga attcgccgtc gggaacgtgg aggctgcccg agccgctgcc cgcgccgcgc
     1861 gcgaaacgct cgtacacctt agagccgcgc cgctcgcgtt ggtcgtctgc aatctcgccg
     1921 cgtacgcgat ggcggtggac gagctcgacg aagctcgcga cctgcttcgc gaagcgctcg
     1981 atctcctgcg cgagtccggg gcgcgttgga tgacgaccgc actcgaacat cacgcaatgc
     2041 ttgccggatt gatcggcgac cacgagcgcg cggcgatttt ggtcggcttc accaacgcgc
     2101 gctacgcggg taacgacacg cgtcagacta ccgagcgata cggttacgag cggctcatgg
     2161 gtcttctgac ccgggcctac gacgaaaccg aacttgcccg acagatgagt gcgggagcct
     2221 ctttgaacgc cgagcaggcg ctcgagcaag cggcggcgat tacccaacat cacgaccggc
     2281 ggcgtcggcc gccttcacag aggagtacca accatgggca gcgcgatgca cgaacaggca
     2341 gccggaaaac ttgacgccca cgggcgcgcg atcgacgagc tacaccagca ccttgccgct
     2401 acgccgggtg tcgacaaagc gcgcttgcaa acggccgtcg acaagtacaa agccgcgtac
     2461 aagcagttcc gcgacgacgc gcaaggctgt atgaactagg agccgttagg agcggagagc
     2521 agcctcgatc gcgaccccgt ttgggatacc gagccccgtg cacggattcc agcctgggcc
     2581 ggcatcgaag cgtccgttgt tcccctgagt aacggcgccg aaaagcgtgc cggcctgccg
     2641 gtaaaggatc ggcgtaaaaa acccaagcgg aacgccgaga cgctgattga ttcgcgcgac
     2701 taacgcggcc cacaccggcg cgacggcgct ggtacctccc atggccagct ccgtcccgtc
     2761 gaccaccacg tagtatccgg gcgactgctg cgccgccacg tcgggaacgc cgcggccggc
     2821 cggcatgccg taatgcgccg cgcagtccgc gcccgtcgct tgccaatcgg gtaccgggaa
     2881 gcgggcgctg aagccgccgc cgctgccttg ccacgcgacc tcccccgcgg ggagaatcgc
     2941 cgtcgctccg cacgcgacgg cgaacggact cgaggccggc gccagcacgt gagggctgcc
     3001 gtggatgtcg agagccgcgc cgtcgtcgcc cgaagagcac gcaacgctga tgccgagcag
     3061 cgccgccgct gcgaacagct cgtcggcaat atccaacgct accggtgtcc agacattctc
     3121 cggccacccg tagctgacgg acacgacgtt cggggcgtac tcgtcgccga aaacggcctc
     3181 gcgcagcgcg tcgaggaagc cgcgttcgct gtcgggtgca tcgtagacga cgacgcgcgc
     3241 ttgcggtgcc agcgatgcga cgacttgaac gtcgagcgcc gcttcgacgt ccttggccgt
     3301 cacggcggcg tgcgcgacca tcgtccgatc gaccggtttg acgaaggggt gctgcgccga
     3361 aacgccttga gcggccatgc agcgatcgaa gtcctcgccg ttaaagccgc cgcgcagctg
     3421 cacgatcccg atcgtctggc cgctgccggc ggactccgga aactggtaac gggatgcgac
     3481 ctcgccggcc atgagcggcg tcgcgtggcg ctcgagcgag cgcacgcgct tcgaacgagg
     3541 ccactggtgc aaaccaaaaa tgccgcgcac gacgccgtcg agctccggta agacgtggag
     3601 cgtgccggat cgatgacgaa agcggcgccc caacccgtct tcgaaaattg caacggtggc
     3661 gccgaatgca cggatcagcc ggtcgatcgg gcccgcgacc gttaccgacc gccaatgcac
     3721 gccggcgacc tcgagaccga agcggtcgca gtaacggcgc accgcgtcga cgtcggccgg
     3781 atcggccccg gtcgcgtgcg cgagcgcctc tcggctcgcg taacggcgtt ctcgcggcga
     3841 aaccgtgccg gtcgcgcgcg cgacctccaa atcgacaccg cccccgcggc ccgcacgcag
     3901 ccagaccgtc aaatccgcct ccgcgtcggc accgagcgcg tcggcgtgcc ggctcccggg
     3961 ccaaacttgt cgcgcggtac ccggaatcgg gatgcggcgt tcgccggaca tccgccggtt
     4021 cgctaccccg acgcggtgct cggatcgatg atcgcggcta cctccgaaat gcggttagcg
     4081 gggaatccgc cctcgcgggc gtgctgacgg atgcgctcct catcggcggc ttcgtaaaaa
     4141 caatacacgc gatcgccggt gacgtaacta tggacccacc tgatgtcgga gcccattccg
     4201 tccagaacgc tgcacgattt ttgcgccacc gcctttagtt cgtcttggga aaggttcccc
     4261 gccccgggga tgtcgcgctc gatgatgtat ctcttcaatg ccacgggctc gctccttata
     4321 gcgcgttagc gcgaaattac gacggcgttg ctgaaggtct tacgcggacc tttgaaggtc
     4381 gagtggaagg ttccggttac cgtcgcgctc gctccgttcg tgcgcgactg gttggtctcg
     4441 tcgatgacga cgacgcattt cgaatcgcag agctggaagc cggcgacttg acccatcatc
     4501 gtgtgcgagg attgaaaatt cgtgaccttc ccggataccg tcacgctctt accgtcgtac
     4561 gtcgacgggc tggcgacgat ggcagacgga gccagcgccg ccgccgcggc cgtcagcggc
     4621 acggcgacgg cgagaacgag cagagcgatc gttcgcacgt tacattcctc ccggcatcat
     4681 gttgcaatcg atcatcttat tcagcagcgg cgcggcgacg atgttcacga aatcggtgcg
     4741 cgcctgcgga ttgcccttga gcttagacgc catggggctc gaggttccgg agccggaac
//
LOCUS       DASUCJ010001043         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_240112, whole genome shotgun sequence.
ACCESSION   DASUCJ010001043 DASUCJ010000000
VERSION     DASUCJ010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240112"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFU90_12320"
     CDS             complement(<1..827)
                     /locus_tag="VFU90_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480618.1"
                     /translation="MALAVGCSGGTQGTGGGARFVPQGGGSQGKIAHVVIVVQENRSF
                     DNFFDCFPGTDCVKTAPGRGPKPGPTSPASPCPAMASPSPGPTPKPIAIKFNVPLISY
                     DPDHRYCPAFVVEYDNGKLDGFYWDGGIQPNQPDGTYPYRVVAEKQIQPYWDMATQYV
                     LADRAFATEASGSFTAHQDLIRGDTKISTSASAVDYPLNSENISNWGCDDAKDSHTPL
                     LTTAGVYIPAFASPSPGPFPCFTYATLRDRLDAAGVTWKYYVPTWPRSGGQMWNAFDA
                     "
BASE COUNT          243 a          423 c          412 g          255 t
ORIGIN      
        1 gcatcgaacg cgttccacat ttggcccccg ctgcggggcc acgtcggcac gtagtatttc
       61 caggtaacgc cggccgcgtc gagccggtcg cgaagcgtcg cgtacgtaaa acacgggaac
      121 ggacccggcg acggcgaggc aaatgccggg atgtagacgc ctgcggtcgt cagcagcggc
      181 gtgtgcgagt ccttggcgtc gtcgcagccc cagttcgaaa tgttttcgga gttgagcggg
      241 tagtcgaccg cgctcgcgct cgtcgagatt ttcgtatcgc ctcgaatgag atcctgatgg
      301 gcggtaaagc tcccgctcgc ttcggtagcg aacgcgcgat ccgccaacac gtactgggtc
      361 gccatatccc agtacggctg gatctgcttt tcggcgacga cgcggtaggg atacgtgccg
      421 tcgggttgat tcggctggat gccgccgtcc cagtagaatc cgtccagctt gccgttatcg
      481 tattccacca cgaaggccgg gcaataccga tggtcgggat cgtaggaaat caatggaacg
      541 ttgaatttta tcgcgatcgg tttcggcgtc ggcccgggtg acggcgacgc catcgccggg
      601 cacggactgg ccggcgacgt cggccccggc ttcgggccgc gtcccggcgc ggtcttcacg
      661 caatcggttc ccggaaagca gtcgaagaaa ttgtcgaagc tgcggttctc ctggacgacg
      721 atcacgacgt gcgcgatctt cccttggctt ccaccgcctt gcggcacgaa ccgcgctccg
      781 ccgcccgttc cctgcgttcc gccggagcag ccgacggcta gcgccaaaca gattaagcaa
      841 gcgaaaccga tagaaacgtt gcgcatgctg aaagaatttc cgccgcacgc tcgagggccc
      901 tctcaaatgc acgcaatttg catatcgcct gctccccttg accggcaagg cgcagcgtgt
      961 taaagtgcgt tcaatgtctt tcgcttcgac ggccacgatg atgccccgca tgtgttggat
     1021 gcgcaagtat ccggcgaggg ccgccgtgcg tagcgtcagg taaaaacgaa acaaccaaag
     1081 acgcgttctg cacgcccctc gcccaaatcg gcggggggcg tgttcgtttc ctgctttccc
     1141 atcgcaacag gaggctagga agaaattgaa tttgaccgcc ggaatcggct tactcggctg
     1201 cggcacggtc ggtgccgccg tcgctgatcg cctgcaacgc tcgcacgagc gcattcgccg
     1261 tcacgccggc gtcggttacg agctgcgcgc cgtcgccatt cgcgacgctc gaaaactgcg
     1321 cccgcactcg ctc
//
LOCUS       DASUCJ010001044         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_790438, whole genome shotgun sequence.
ACCESSION   DASUCJ010001044 DASUCJ010000000
VERSION     DASUCJ010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790438"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFU90_12325"
     CDS             <1..437
                     /locus_tag="VFU90_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5480619.1"
                     /translation="VATAGAGDAAYVRDLGAAETIDFRTERFEDRVRDVDAVIDTIGG
                     DTQDRSFAVLARGGVLVSSVSAPSPEKAAERGVRVAYFIVDVTASALATIARLIDDGK
                     LAPDLGVVLPLEAAREAHEMLAGSLPHPRGKIVLGVAALAPP"
     gene            complement(450..>1283)
                     /locus_tag="VFU90_12330"
     CDS             complement(450..>1283)
                     /locus_tag="VFU90_12330"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480620.1"
                     /translation="GQESPDPTGPFPCFGPGGPRPDVHYPALAQRVDEKNLSWRLYAH
                     SLCSSIVGLDVNAGVRYSDLWPTKPDMARCHSHESAFFPTPVNTANFRAPQDTFLSDV
                     AGSKGKLANVTWILPGPITSDHPGVPFGYSGPSWVADVVNAIGKSKYWDSTVIFIFWD
                     DWGGFYDHVRPYVVRDQEGPGFRVPLLVVSPYAKRGHVAKTDVEFATLLKFTEQTFGL
                     RSLGATDDSPYLNDLDDFFQSSPQPFTKIKLPNYYLCSESAHPRPNPATHSRWLEMDG
                     D"
BASE COUNT          202 a          420 c          437 g          224 t
ORIGIN      
        1 tcgtcgctac cgccggcgcc ggggatgccg cctacgtccg cgatttgggc gccgctgaaa
       61 cgatcgactt ccggacggag cggttcgagg atcgcgtccg cgacgtcgac gccgtgatcg
      121 atacgatcgg cggcgacact caggaccgtt cgttcgcggt cctcgcacgc ggcggcgttc
      181 tcgtttcgag cgtttcggcg ccgtcgccgg aaaaggccgc cgagcgcggc gttcgcgtcg
      241 cctacttcat cgtcgacgtc accgcgtctg cgctcgcgac gatcgcacgg ctgatcgacg
      301 acggcaagct cgccccggac ctcggcgtcg tcctgcccct cgaggcagcg cgcgaggccc
      361 acgagatgct ggccggctct ctgccgcatc cccgcgggaa aatcgtgctc ggcgttgccg
      421 ccctcgcgcc gccgtagcgg aacgccgggt tagtcgccgt ccatctcgag ccagcgcgag
      481 tgcgtagccg ggttcggtcg cggatgtgcg ctttcgctgc agagatagta gttcggaagc
      541 ttgatcttcg taaacggctg cgggctcgat tggaagaagt cgtcgagatc gttcagatac
      601 gggctgtcgt cggtggcgcc gagcgaacgc agcccgaacg tctgttccgt gaacttcagc
      661 agcgtcgcga actcgacgtc ggtcttggcg acgtgcccgc gcttggcgta cggcgaaacg
      721 accagcagcg gaacgcggaa tcccgggcct tcctgatcgc gcacgacgta gggacggacg
      781 tgatcgtaga agcctcccca gtcgtcccaa aagatgaaga tcacggtgga atcccagtac
      841 ttgctcttgc cgatcgcgtt gaccacgtcg gccacccagc taggcccgct gtagccgaac
      901 gggacgccgg ggtgatccga ggtgatcggc cccggcaaga tccaggtgac gttggccagc
      961 ttccccttgc tgccggcgac gtcgctcaga aacgtgtcct gcggcgcgcg gaagtttgcc
     1021 gtattgaccg gcgtcgggaa aaatgcgctt tcgtggctgt ggcaacgcgc catgtcgggc
     1081 ttcgtcggcc agaggtcgct atagcggacg ccggcgttga cgtccagccc gacgatactg
     1141 gaacagagcg agtgcgcgta cagccgccac gagaggttct tttcgtcgac gcgctgcgcg
     1201 agcgccggat agtgcacgtc gggcctcggt ccgccgggcc cgaagcaggg gaacggcccg
     1261 gtcggatccg gcgactcctg gcc
//
LOCUS       DASUCJ010001045         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1478289, whole genome shotgun sequence.
ACCESSION   DASUCJ010001045 DASUCJ010000000
VERSION     DASUCJ010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478289"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1291
                     /locus_tag="VFU90_12335"
     CDS             269..1291
                     /locus_tag="VFU90_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5480621.1"
                     /translation="MRRTLPLVLAFALAACAGTGSGTHAVPAFPNPAFQSADRGGASM
                     DWPVMAGGAADQYALQALDFYPNVITIDAGDTITYQIAGGTGGDAHTVSFVPAKMKMP
                     SPLDPLDLVPRGGTIITGKKFVNSGILVGGQTFTLSFPKPGTYRIVCLFHEPAMEGTV
                     VVQKAGTHYPHGVKYYQRIGESQQRNDLGAARASVALFPFKPGGTMLAAGIAPGLVQY
                     PPSDATVLRYVDTTDASQLQTSGNLTIKVGTVVTWVNETANEPHTITLNVAGQKKLPN
                     IPPDPPVNFKRHGITDYDGSQVVNSGTFLGGTAVRLRFTKAGKFFYGCLYHDNSGMDG
                     TITVTK"
     gene            complement(1275..1562)
                     /locus_tag="VFU90_12340"
     CDS             complement(1275..1562)
                     /locus_tag="VFU90_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU5480622.1"
                     /translation="MLLEVIVKPSSRESGIALDGGTIVARVRERAVDGAANAACVRLL
                     AGCLAVAPSSVVLVRGARARRKLFAVAGLTAAEARRRIEARFSGVNATSLP"
     gene            complement(1586..>2087)
                     /locus_tag="VFU90_12345"
     CDS             complement(1586..>2087)
                     /locus_tag="VFU90_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480623.1"
                     /translation="NDVSRYGYDVVLGADLLGSTALDIDAAAHTVKLGAATPANSIAI
                     PLAFENFVPVVNVHLGTIDARLAVDTGDESNINLAYDFYSKHSTLFAVTEHRLVSGIG
                     GTSVELIGSIPEVTIGDFRTGPQKIGTTRTLQGTAFGHLGAAFLQQFDVQLDYAAGEL
                     HLAPKP"
BASE COUNT          407 a          616 c          695 g          369 t
ORIGIN      
        1 cgacgtcgcg ctcgcgctcg aacggagcgt tgggttcgaa ctccagcact tgttgggccg
       61 gacgttcgta cagccggatc gtgccgcatc gtaagcagtg ggcggatcgc gcgaaatata
      121 ggacctacga ctcttcgaag ctgggaccta aggctctcga atgcgggcaa ttcggccgtg
      181 cgcggctagg acgcggttcg gatcggacgt tccatccggc cacatttgag cgtataattc
      241 acgccccaaa gctacaaggg agggtttcat gcgaagaaca ctacctctcg tgctcgcgtt
      301 tgcgctggcg gcgtgcgcgg gaaccggaag cggaacgcat gcggttccgg cctttcccaa
      361 cccggcattc cagagtgcgg atcgcggcgg agcatccatg gattggccgg tgatggcggg
      421 cggggcggcc gatcagtacg cgcttcaggc gctcgacttc tatccgaacg tcatcacgat
      481 cgacgcgggc gatacgatca cgtatcagat cgcgggtgga accggcggcg acgcgcacac
      541 ggtgtcgttc gtgcctgcca agatgaagat gccgtcgccg ctcgacccgc tggacctggt
      601 cccgcggggt ggaacgataa ttaccgggaa gaaattcgtc aattcgggaa ttctcgtcgg
      661 cggtcagacg ttcacgctga gttttccgaa gcccggaacc tatcgcatcg tctgtctgtt
      721 ccacgaaccg gcgatggagg gcaccgtcgt cgtacagaag gcgggcacgc actatccgca
      781 cggcgtcaaa tactatcaga gaatcggcga atcgcaacag cggaacgatt taggcgcggc
      841 gcgcgcttcg gtggcgctct tcccctttaa gccgggcgga acgatgctgg cggccggaat
      901 cgctccgggg ctggtgcagt acccaccgtc ggatgcgacg gtactgcgct acgtcgatac
      961 gacggatgcc agccagctcc aaacgtcggg aaatctgacg atcaaggttg ggacggtcgt
     1021 tacgtgggtg aacgaaactg caaacgaacc gcacacgatt acgttgaacg ttgccggcca
     1081 aaagaaactg ccgaacattc ctcccgatcc gccggtcaac ttcaagcgcc acggaatcac
     1141 ggattacgac ggaagccagg tcgtgaactc cggcacattt ctcggtggga ctgcagtccg
     1201 gctgcgattc accaaggccg gaaagttctt ctacggttgc ctctatcacg acaactcggg
     1261 aatggacgga acgattacgg taacgaagta gcgttaacgc cgctgaagcg cgcttcgata
     1321 cgccggcgcg cttcggcggc cgttaacccg gcgacggcga agagtttgcg gcgcgctcgc
     1381 gcgccgcgca cgagcacgac cgacgatgga gcgactgcga gacagccggc gagaagccga
     1441 acgcacgcgg cgttggcggc gccgtccacg gcacgctcgc gcacgcgcgc gacgatcgta
     1501 ccgccgtcga gcgcgattcc ggactcccgt gaggacggct tgacgatgac ttcgagaagc
     1561 atggcgcggt taggttgggg ctgttctaag gtttgggcgc gaggtgcagc tcgccggcgg
     1621 cgtagtcgag ctgcacgtcg aactgctgga gaaatgcggc gcccagatga ccgaaggcgg
     1681 tgccctgcag cgtacgcgtc gtcccgattt tctgcgggcc ggtgcgaaaa tcgccgatgg
     1741 taacctcggg gatggagccg atcagctcga cgctggttcc accgatgccg ctgaccaagc
     1801 ggtgctcggt gacggcaaac agcgtcgagt gcttcgagta gaagtcgtac gccaggttga
     1861 tgttcgattc gtcgccggta tcgaccgcca gccgcgcgtc gatcgtcccc aaatggacgt
     1921 ttacgaccgg aacgaagttc tcgaacgcca gcgggatcgc gatcgagttg gcgggcgtgg
     1981 cggcgccgag cttgacggtg tgcgccgcgg catcgatatc gagtgcggtc gagccgagca
     2041 agtcggcgcc gagtacgacg tcgtagccgt agcggctgac gtcgttg
//
LOCUS       DASUCJ010001046         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1279707, whole genome shotgun sequence.
ACCESSION   DASUCJ010001046 DASUCJ010000000
VERSION     DASUCJ010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279707"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..784
                     /gene="rplB"
                     /locus_tag="VFU90_12350"
     CDS             2..784
                     /gene="rplB"
                     /locus_tag="VFU90_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5480624.1"
                     /translation="MTTIDFSELSKVEPERSLVEVRKKHSGRNNNGHITVRHKGGGTR
                     RQYRIIDFKRTKDAIPAKVATLEYDPNRSARIALLNYRDGEKRYILAPLGLKVGDVVE
                     SGPSADIKTGNCLPIKNIPVGTVIHNIELRPGQGGKLVRSAGGSAQLMAKEDDYAQVR
                     MPSGEVRKILVVCRATIGQLGNVEHENEVIGKAGRQRHRGKRPSVRGIAMNPVDHPHG
                     GGEARSTSGRPPTTPWGQMTMGKKTRRNKRTQNMIVRKRGSK"
     gene            784..1059
                     /gene="rpsS"
                     /locus_tag="VFU90_12355"
     CDS             784..1059
                     /gene="rpsS"
                     /locus_tag="VFU90_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5480625.1"
                     /translation="MGRSLKKGPFVADHLLKKIDALNERREKRVVKTWSRATTIVPAM
                     VGHTIGVHNGKAHVPVFITENMVGHKLGEFAPTRNFRGHGGDKASVK"
BASE COUNT          252 a          359 c          351 g          162 t
ORIGIN      
        1 catgaccacg atcgatttct cggagctgag caaagtcgag cccgagcgtt cgctcgtcga
       61 agtccgcaag aagcattccg gacgcaacaa caacggccac atcaccgttc gccataaggg
      121 cggcggcacg cggcgccagt accgcatcat cgatttcaag cgcaccaaag acgcgattcc
      181 cgcgaaggtt gcgacgctcg agtacgaccc gaaccgctcc gcccgcatcg cgctgctcaa
      241 ctaccgcgac ggcgagaagc gctacatcct cgcgccgctc ggtctgaagg tcggcgacgt
      301 cgtggagtcg ggtccgagcg cggacatcaa gaccggcaac tgcctgccga tcaagaacat
      361 cccggtcggt accgtcattc acaacatcga gctgcgtccc ggtcagggcg gcaagctcgt
      421 gcgcagtgcc ggcggctccg cgcagctgat ggccaaggaa gacgactacg cgcaggtgcg
      481 catgccgtcg ggcgaagtcc gcaagatcct cgtggtctgc cgcgccacca tcggccagct
      541 cggcaacgtc gagcacgaaa acgaagtcat cggtaaagcc ggccgtcagc gccatcgcgg
      601 caagcgcccg agcgttcgcg gcatcgcgat gaacccggtg gatcacccgc acggcggcgg
      661 tgaggcgcgt tcgacctccg gtcgtccgcc gaccacgcct tggggtcaga tgaccatggg
      721 caagaagacg cgccgcaaca agcgcacgca aaacatgatc gtccgtaaac gtggtagcaa
      781 ataatgggac gtagcttaaa gaagggtccg ttcgtagcgg atcatctgct caagaaaatc
      841 gatgcgttga acgagcgccg cgaaaagcgc gtcgtgaaga cgtggagccg tgcgacgacg
      901 atcgttccgg cgatggtcgg ccatacgatc ggcgtgcaca acggcaaagc gcacgttccg
      961 gtcttcatta ccgagaacat ggtcggccac aagctcggcg agttcgcgcc cacgcgcaac
     1021 ttccgcggcc acggcggcga taaggcatcg gtgaagtagc atggcagagc aaacgcaaga
     1081 acagcgcacc gaagcggtcg cgcatctgaa gttcgcgcgc atgg
//
LOCUS       DASUCJ010001047         1432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1478297, whole genome shotgun sequence.
ACCESSION   DASUCJ010001047 DASUCJ010000000
VERSION     DASUCJ010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478297"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFU90_12360"
     CDS             <1..1058
                     /locus_tag="VFU90_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5480626.1"
                     /translation="TVRVNGDLVALETQPLKPEELKSLAEQIMTPRQVKEFAEHKEAD
                     FAIGVPGVGRFRTNVYQQRGTVAFAFRAIPYEVKTLQELNLPAVMEEIALRPRGLVLV
                     TGVTGSGKSTCLAAMLNHINASRRVNIITIEDPIEFLHRDNLANISQREVGNDTLSFG
                     SALRHVLRQDPDVILIGEIRDMETLDTALKAADTGHLVFSTLHTTDATQTINRVISFF
                     PPHQHDEVRHLLSTALQAVVSLRLVPRKDAKGRVPACEVLINTAAVADNVRDLSKQLN
                     IPDLIAEGSIQYGMQSFDQSLFSWYQNGMISYESAIFYATNPSEFALKVSGVDASGSK
                     LGGPGAAAGVESIGLTS"
     gene            1058..>1432
                     /locus_tag="VFU90_12365"
     CDS             1058..>1432
                     /locus_tag="VFU90_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5480627.1"
                     /translation="MFQKVLIANRGEIALRVIRACKELGIATVAVFSEADRECLHVRF
                     ADDDVCIGRPPSRDSYLNIPRIIAAAEITGADAIHPGYGFLAENAEFAEIVRTSGITF
                     IGPTADQIREMGDKAAARTRAQS"
BASE COUNT          261 a          484 c          465 g          222 t
ORIGIN      
        1 cgaccgtccg ggtcaatggc gatctcgtgg cgctggaaac gcagccgctg aagcccgagg
       61 agctgaaatc gctggccgag cagatcatga cgccgcgcca ggtgaaggag ttcgccgagc
      121 acaaggaggc ggacttcgcg atcggcgtgc cgggcgtcgg gcgcttccgc accaacgtct
      181 accagcagcg cggcacggtc gcgtttgcgt tccgcgccat cccctacgag gtgaagacgc
      241 ttcaggagct caacctgccg gccgtgatgg aggagatcgc gctgcggccg cgcgggctgg
      301 tgctcgtcac cggcgtgacg gggtcgggga agtcgacgtg tctcgcggcg atgctgaacc
      361 acatcaatgc cagccggcga gtcaacatca tcacgatcga ggacccgatc gagttcctgc
      421 accgcgataa cctggccaac atctcgcagc gcgaggtcgg caacgacacg ctgtcgttcg
      481 gctcggcgct gcgccacgtg ctgcgccagg acccggacgt gattctcatc ggcgaaattc
      541 gcgatatgga gacgctcgat acggcgctca aggcggccga caccgggcat ctcgtcttct
      601 cgacgctcca cacgaccgac gctacgcaga cgatcaaccg cgtcatttcg ttcttcccgc
      661 cgcaccagca cgacgaagtt cggcacctgc tgtcgacggc gctgcaggcg gtggtgtcgc
      721 tgcggctcgt gccgcgcaag gacgccaagg ggcgcgtgcc ggcctgcgag gtgctcatca
      781 acaccgcggc cgtggccgac aacgtccgtg acctgtccaa acagctcaac attcccgacc
      841 tcatcgccga gggatcgatc cagtacggga tgcagagctt cgaccagtcg ttgttcagct
      901 ggtatcagaa cggcatgatc agctacgaga gcgcgatctt ctacgcgacg aatccgagcg
      961 aattcgccct caaggtgtcg ggtgtcgacg catcggggag caagctgggc ggaccgggag
     1021 ccgcggccgg ggtcgaatcg atcggcctca cgagctgatg ttccagaagg tgctgatcgc
     1081 gaaccgcggg gaaatcgccc tgcgggtaat tcgcgcctgc aaagagctcg gcatcgccac
     1141 cgtcgccgtc ttctccgagg cggatcgcga gtgcctgcac gtgcggttcg ccgacgacga
     1201 cgtgtgcatc ggccgcccgc cctcccgcga cagctacctc aacatcccgc gcatcatcgc
     1261 cgccgccgaa atcaccggcg ccgacgcgat tcaccccggc tacggcttcc tcgccgagaa
     1321 cgccgagttc gccgagatcg tgcggacgtc gggcatcacg ttcatcgggc cgacggcgga
     1381 ccagatccgc gagatgggcg acaaagccgc ggcccgcaca cgcgcgcagt cg
//
LOCUS       DASUCJ010001048         6661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1264427, whole genome shotgun sequence.
ACCESSION   DASUCJ010001048 DASUCJ010000000
VERSION     DASUCJ010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264427"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFU90_12370"
     CDS             complement(<1..609)
                     /locus_tag="VFU90_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480628.1"
                     /translation="MLIRIGAALAALALTYSAAVAATPAPKATLSPMPQGAEVAFVTS
                     IQSDLNKRFPTPQAAEAAGYFRYGNEDSTGAISYANLQWQSANPQSPSQLWYDVHGNL
                     LGADFSVLQSGSPAPPELWGINYRRWVSFREHVHYILAGPNGTETYGATSAKKFAAAG
                     GNVDDPQAATIVKMGLAKNVAEVKKVFLFPSLWDLIVWIKPNP"
     gene            complement(627..1286)
                     /locus_tag="VFU90_12375"
     CDS             complement(627..1286)
                     /locus_tag="VFU90_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480629.1"
                     /translation="MPFDSSLDFVLDDAISSKSSRAGQLVAAHLKNALVVGGRVVAPA
                     GTAGKIRIVDVSPADIGDVYGFVDIYFEPLDLPDGRKLPLRAPVGRLEPHVSAGHEST
                     VAWEDTIEDQVIPYHFLYHIFRSGKNFVLKAGSELPAHTDATLTALPNGTVAIQTPRP
                     LQQTLAVPKSAFPVEPAATPFGAGETKSRKTPAPVPTQTPTPWPTVEPPTPSPAAGAT
                     P"
     gene            complement(1441..1776)
                     /locus_tag="VFU90_12380"
     CDS             complement(1441..1776)
                     /locus_tag="VFU90_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480630.1"
                     /translation="MSGIPEQPKRAGGMSVREAGQKGGETVKQKYGAEFYEQIGRKGG
                     LATKEAHGHEFYEQIGKKGGKKGGEATRDRYGPHFYERIGQKGGQKVKQLIEEGKRAA
                     AAAREKRAS"
     gene            1930..3405
                     /locus_tag="VFU90_12385"
     CDS             1930..3405
                     /locus_tag="VFU90_12385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5480631.1"
                     /translation="MTCVVASAAAPPNPYLEILTLESKRSLGNGELARLLDSPDARVA
                     ARAALAVGRTKQASGEPLVAAHLADPRTAVRAFSVYSAGLLATGDQATAIVGALGDPA
                     GAVRVAAADAVARYEAAGRFGSAEIPAQQALERALASDSSAIVRARAAVALVEFRSGP
                     RVPEAAKALTAAYRGDRDPGVRWHAMWAIYRGFAVAAGTAVVEPALHDRDELVRIEAV
                     RAMARFKDARLVGAVEPLLADRSWRVQEQASETLRVLEGKKPSEHWTAIPAFVHVPAV
                     RPDPLRGLPALPAGPTPPAGAPLPEQAILEPALDPATAAEMTSPAAGPHPRVRIVTSK
                     GNLYVTLFPEWAPMTVANFLNLAQRGYYDGNRWFRIVPDFVVQTGDPNDNGNGDAGYS
                     LGAEENPLEQRSYVISMGLNYDDKTQTPIRDSAGTQYYVTLSPQLHLDRDFTVFGEIT
                     GGFDVLANLVERDRVLRVERIAGANVKTPASNPAAASPAPD"
     gene            complement(3356..3796)
                     /gene="dtd"
                     /locus_tag="VFU90_12390"
     CDS             complement(3356..3796)
                     /gene="dtd"
                     /locus_tag="VFU90_12390"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5480632.1"
                     /translation="MVQRVSSARVRVGERVTGEIGTGLLAFVGVGVDDGPADADWLAE
                     KIAGLRVFADEEGRMNRAVPDAGGAVLLVSQFTLHGDARKGRRPSFVRAAKEPLARDL
                     FERVGAQIASRGLRVAYGEFGADMDVELVNRGPVTLLLDSKREF"
     gene            complement(3811..4704)
                     /locus_tag="VFU90_12395"
     CDS             complement(3811..4704)
                     /locus_tag="VFU90_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5480633.1"
                     /translation="MALLDRLLAPDSKFQKRFFFLAKRFVPGETIADAVATVRDLNRQ
                     GMTASLDFLGEDVRERNAATDTTGVYLKILDALRETGVDSNVSVKLTAIGLLIDESFA
                     LENLLRIAEHARRNADPFVRIDMEGSPVTEATLRVFERAFATDRNVGIVLQAYLKRTA
                     EDVERAIALGARVRLCKGAYNEPPAIAYKSMPEIRTNFLRLAERLLERGTYPGIATHD
                     RRLIAAVRDFAGRRSIARDRFEFQMLHGCRPQLQRALVAGGYRMRVYVPFGTHWAGYF
                     YRRVMERRENALFALSSMFSR"
     gene            complement(4713..5492)
                     /locus_tag="VFU90_12400"
     CDS             complement(4713..5492)
                     /locus_tag="VFU90_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5480634.1"
                     /translation="MMVPRPLPSPLFLIAAIALLGAVVGPVAAVLFSMSPHEALATFD
                     AAGRDALRVSLAASVGATLVATLLGVPAGYYFSRLPRRLRAAAVFALALPLAFPPVAS
                     GLMLVYVFGIRSGFGQWLAAHGVMVPDTLLGVGVAEFFVSGSFVAIAATAAFSALDPT
                     YEDAARTLGASEWRIFTRIALPGAAASVGAGVTFAWLRAIGEYGATSIVAFHPTSLPV
                     ALYVTLSASGVREALALCYGFIVLAAAALAGAWILRRGVVL"
     gene            5496..6059
                     /locus_tag="VFU90_12405"
     CDS             5496..6059
                     /locus_tag="VFU90_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HEU5480635.1"
                     /translation="MTAVLKVLFVALSLSLDVFAVSVGVGVRGVPAGLKWRIGFSFAF
                     AEIAMNLVGAGLGLAAGKLLGETAGYIGFVALIALGIYMMKESRSDLSGSPKLDLSKG
                     WGLAVASLSISLDSLGIGFSILYIGVQPAIALAAIGTVSVCSTILGLALGERLGGLAE
                     RHAAFLGGILLTLTGLTFGILKALHID"
     gene            6073..>6661
                     /locus_tag="VFU90_12410"
     CDS             6073..>6661
                     /locus_tag="VFU90_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480636.1"
                     /translation="MTVGQSLLDLAQAAGADAVFVVGIGKNVGKTVTARAVYEAAHAA
                     GISTGITSIGRDGELADAGDARPKPRLFLRPGTVVATALGVLPRSPACEMLDVSSLQT
                     AAGPVAYARVVHAAHYELVGPPTASGMREVVERLRAHARFTIVDGAIDRVAATAGSTG
                     AIVVAAGASAAGTPAEAVDAIRALALRLGVAGYDEK"
BASE COUNT         1088 a         2172 c         2275 g         1126 t
ORIGIN      
        1 gggattgggc ttgatccaaa cgatcagatc ccaaagcgac gggaacaaga agactttttt
       61 cacctcggcg acgttcttgg cgagacccat tttgacgatc gtcgcggctt gcggatcgtc
      121 gacgttaccg ccggcggcgg cgaacttctt cgcgcttgtc gcgccgtagg tctcggtgcc
      181 gttcggcccg gcgagaatgt agtgcacgtg ttcgcggaac gaaacccagc gccggtagtt
      241 gatgccccag agttcgggcg gcgccggcga gccgctctgc agaaccgaaa aatcggcacc
      301 tagcaaattt ccgtggacgt cgtaccacag ctggctgggc gactggggat tcgcgctctg
      361 ccactgcagg ttggcatacg aaatcgcgcc ggtgctgtct tcgttaccgt agcgaaaata
      421 gccggcggcc tccgccgctt gcggcgtggg gaagcgcttg ttcagatcgg attgaatcga
      481 cgtgacgaat gcgacttcgg cgccctgcgg catcggcgaa agcgtcgctt tcggagcggg
      541 cgtggccgcc actgcggccg agtaggtcaa ggcgagggcg gccagggcag ccccgattcg
      601 aatcagcacg aaaacgaccc ctttctctat ggcgtagcgc ctgcggccgg tgacggagtc
      661 ggcggctcca cggtcggcca cggcgtaggc gtttgtgtcg gcacaggtgc cggggttttc
      721 ctgctcttgg tctcgccggc accgaacggc gtagccgccg gctcgaccgg gaaagccgat
      781 ttcggaacgg ccagcgtctg ctgcagcggt cgcggcgttt ggatcgcgac cgtgccgttg
      841 ggaagcgcgg tcagcgttgc atcggtatgc gccggaagct cggagccggc tttgagaacg
      901 aaattcttcc ctgagcggaa gatgtggtag agaaagtgat acgggatgac ctgatcttcg
      961 atcgtgtctt cccacgccac cgtcgactcg tgcccggccg agacgtgcgg ttcgagcctc
     1021 ccgaccggcg cgcggagcgg aagtttgcgg ccgtccggaa gatcgagcgg ttcgaaataa
     1081 atgtcgacga agccgtacac gtcgccgatg tcggccggcg aaacgtcgac gatccggatt
     1141 tttccggcgg tgcctgcggg tgcgactacc cggccgccga cgaccagtgc attcttcaaa
     1201 tgggctgcga cgagctgacc ggcgcgcgac gatttcgacg agatcgcgtc gtcgagtacg
     1261 aaatcgagac tcgaatcgaa tggcaaaacg cccggcggag gcgggctcgg cgtttgtgaa
     1321 gcggtcggtt ccggcgtggg cgagaccggg tacgcgtaaa ccgatcccgc gccaagcacg
     1381 gataagagcg ctagggcgaa gacggcgccg gcaatgctgc gcggcgcgcg ccggccgaaa
     1441 ttagctcgcg cgcttctcgc gagcggcggc ggcggcgcgc ttaccttctt cgatgagttg
     1501 cttgactttt tggccacctt tttgtccgat gcgttcgtag aagtgcggtc cgtagcggtc
     1561 gcgggttgct tcgccgcctt tctttccgcc cttcttgccg atctgctcgt aaaattcgtg
     1621 accgtgcgcc tctttcgtcg ctagcccgcc cttacggccg atctgctcgt agaattccgc
     1681 accgtacttt tgcttgacgg tctcgccgcc tttctggccg gcctcgcgaa ccgacattcc
     1741 accggcgcgt ttgggttgtt cggggatgcc tgacattttt tcgttcacct tgctttgctc
     1801 ggtcttccga gcggaaggac gacgcttacc tgcgtctgct ctcactatag tagaacgcag
     1861 gtttgggctc gaaggttggc gtccatgttc aagcccggtc tcttttttag cgggatagcg
     1921 acccttgtcg tgacctgcgt cgtggcctcc gcagccgctc cacccaatcc ctatctagag
     1981 attctaaccc tcgagagcaa gcgttccctg ggtaacgggg agctggcgcg cctgctcgac
     2041 tcgcccgacg ctcgggtcgc cgcccgggcg gcgctggccg tcgggcgcac caaacaggcc
     2101 tccggtgagc cgctggtggc ggcccatctg gccgacccga ggaccgcggt acgcgccttc
     2161 agcgtctact cggccgggct gctggcgacc ggcgaccaag ccacggcgat cgtcggagca
     2221 ctgggcgatc ccgccggcgc ggtgcgggtg gcggccgccg acgccgtcgc acgctacgag
     2281 gctgcaggac ggtttgggag cgccgagatt cccgcccagc aggcgctaga gcgagcgctc
     2341 gcgtcggata gctcggccat cgtccgggcg cgggcggccg tggcgttggt ggaatttcga
     2401 agcggtccgc gggtgccgga agcggcgaag gccttaaccg cggcctaccg gggcgaccgc
     2461 gacccgggcg tccggtggca cgcgatgtgg gcgatctatc ggggttttgc ggtcgccgcc
     2521 gggaccgcag tcgtcgagcc cgccctgcac gatcgcgacg agctggtgcg gatcgaggcg
     2581 gtccgcgcga tggcgcgctt caaggacgcg aggctggtgg gggccgtgga gccgctgctc
     2641 gcggaccggt cgtggcgggt ccaggagcag gcgtccgaga cgctgcgcgt gctcgagggg
     2701 aagaaaccct ccgaacactg gaccgcgatt ccggcgttcg tgcacgttcc ggcggtgcgg
     2761 cccgacccgc tgcgcgggct gccggcgctg ccggccggcc ccacgccgcc ggccggtgcg
     2821 ccgctgcccg agcaggcgat tctcgagccg gcgctggacc ccgcgaccgc tgccgagatg
     2881 acgtcgcccg ccgccggacc gcatccgcgc gtccgcatcg tcaccagcaa gggcaatctg
     2941 tacgtcacgc tgtttcccga gtgggcgccg atgacggtcg ccaatttttt gaacctcgcg
     3001 caacgcggat attacgacgg gaatcgctgg tttcgcatcg tgcccgattt cgtggtgcaa
     3061 accggcgatc cgaacgacaa cggcaacggc gacgccggct actcgctcgg cgccgaagag
     3121 aatccgctcg agcagcgcag ctacgtgatc tcgatggggc taaactacga cgacaagacg
     3181 cagacgccga ttcgggattc ggcgggcacg cagtactacg tgacgttgtc gccgcagctt
     3241 cacctcgacc gcgacttcac ggtgttcggc gaaatcaccg gcggtttcga cgtgctggcc
     3301 aacctggtcg agcgcgatcg cgtgctgcgg gtcgagcgga tcgccggcgc aaacgttaaa
     3361 actcccgctt cgaatccagc agcagcgtca ccggcccccg attgaccagc tcaacgtcca
     3421 tatcggcgcc gaattcgccg taagcgacgc gcagcccgcg cgacgcgatc tgcgcgccta
     3481 cccgttcgaa caagtcgcgc gcaagcggtt ccttggcggc gcgaacgaac gacggccgcc
     3541 ggcctttgcg cgcgtcgccg tgcagggtga actgcgacac gagcagcacc gcgccgccgg
     3601 cgtccggcac cgcccggttc atacggcctt cctcgtcggc gaagacgcgc agtccggcga
     3661 tcttttcggc gagccaatcg gcgtcggcgg gaccgtcgtc gactccgacg ccgacgaagg
     3721 ccagcaatcc ggttccgatc tccccggtga cgcgttcgcc gacgcgcacg cgcgcgctcg
     3781 aaacacgctg gaccaccgcg cgcaaacgcg ctaccgcgaa aacatcgagg agagcgcgaa
     3841 cagcgcgttt tcgcgccgct ccatcacgcg gcggtagaaa taaccggccc agtgggtccc
     3901 aaacggaacg tagacgcgca tccggtagcc gccggctacg agcgcgcgct ggagctgtgg
     3961 acggcagccg tgcagcattt gaaattcgaa gcggtcgcgc gcgatcgaac ggcggccggc
     4021 gaaatctctt accgcggcga tcagccggcg atcgtgggtg gcgatgccgg ggtaggtccc
     4081 gcgttcgagc agccgttcgg ccaggcgcag aaagttcgtg cggatctcgg gcatactctt
     4141 gtacgcgatg gcgggcggct cgttataggc gcctttgcag agacgaacgc gggcgccgag
     4201 cgcgatcgcc cgctcgacgt cctcggcggt gcgcttcaag tatgcttgga gcacgattcc
     4261 tacgttacga tcggtggcga aggcgcgctc gaagacgcgc agggtggctt cggtgaccgg
     4321 ggagccttcc atgtcgatgc gtacgaacgg atcggcattg cggcgggcgt gctcggcaat
     4381 ccgcagcaag ttctcgagcg cgaagctctc gtcgatcagc aagccgatgg cggtgagctt
     4441 caccgagacg ttcgagtcca cgcccgtttc gcgcagggcg tcgagaatct tcaggtagac
     4501 gccagtagta tcggtcgcgg cgtttcgctc gcgaacgtct tcgcccagaa agtcgagcga
     4561 ggccgtcatg ccttgccggt tgaggtcgcg aaccgtcgcg acggcgtccg cgatcgtttc
     4621 gcccggaacg aagcgtttgg cgagaaagaa gaagcgtttt tgaaacttcg aatcgggtgc
     4681 cagcaaacga tcgagcaaag ccatgccagg ccctacagta cgacgccgcg gcgcagaatc
     4741 caggctccgg ccagcgccgc cgccgcgagt acgatgaagc cgtagcacag cgctagcgct
     4801 tcgcgtacgc ccgacgcgga aagcgtgacg tacaacgcca ccggcagcga ggtcgggtga
     4861 aacgcgacga tgctcgtcgc gccgtactcg ccgatcgctc gcagccaggc aaacgtcacg
     4921 ccggcaccta cgctggcggc cgcaccgggg agggcgatcc gcgtgaatat ccgccactcg
     4981 ctcgcgccca gcgtgcgtgc ggcgtcttcg tacgtcggat cgagtgccga aaaggccgcc
     5041 gtcgcggcaa tcgcgacgaa cgagccggag acgaagaact ccgccacccc taccccgagc
     5101 agcgtgtcgg gcaccatcac gccgtgggcc gcgagccact gcccgaatcc cgagcgaatc
     5161 ccgaaaacgt agaccagcat caagcccgat gcgaccggcg gaaacgccag cggcagcgcg
     5221 agcgcaaaca cggcggcggc gcgcaggcgg cgtggcaaac gcgagaagta gtacccggcc
     5281 ggtacgccga gcagcgtagc gaccagcgtc gcgccgaccg aagcggccaa cgaaacgcgc
     5341 agcgcgtcgc gtccggccgc gtcgaacgtc gcgagcgctt cgtgcggcga catcgagaag
     5401 agcactgccg ccaccggtcc cacgaccgct cccagcagcg cgatcgccgc gatgagaaag
     5461 agggggctcg gaagcgggcg cggcaccatc atcgcgtgac tgccgtcctc aaggttcttt
     5521 tcgtcgcgct gagtttatcg ctcgacgttt tcgcagtcag cgtcggcgtc ggcgtacgcg
     5581 gtgttcccgc cggtttgaaa tggcgcatcg ggttctcgtt tgcattcgcc gaaatcgcca
     5641 tgaacctcgt cggcgccggc ctcggactgg cggccggcaa gctcctgggc gaaaccgccg
     5701 gctacatcgg cttcgtcgcg ttgatcgccc tgggaatcta tatgatgaag gaaagccgca
     5761 gcgatctgtc cggcagtccg aagctggacc tctcgaaagg atgggggctg gcggtcgcct
     5821 cgctctcgat cagcctcgat tcgctgggca tcggcttctc gattctctac atcggcgttc
     5881 agccggcgat cgcgctggcg gccatcggta ccgtctcggt gtgctccacg atcctggggc
     5941 tcgcgctcgg cgaacggctg ggaggtttgg cggagcgcca cgccgcgttc ttgggcggaa
     6001 ttctgctcac gctcaccggc ttgacgttcg ggattctcaa ggcgctgcac atcgactaac
     6061 gaagtgcggc ccatgacggt tgggcagtcg ctgctcgatc tcgcccaggc cgccggtgcc
     6121 gacgcggtgt tcgtggtggg gatcggaaaa aacgtcggca agaccgtgac cgcgcgagcc
     6181 gtctacgaag cggcgcacgc cgccgggatt tccacgggta ttacctcgat cggtcgcgac
     6241 ggcgagcttg ccgacgcggg cgacgcgcgt ccgaagccgc ggctctttct gcggccgggc
     6301 acggtcgtcg ccaccgcgct cggcgtgctg ccgcgttcgc cggcgtgcga gatgctcgac
     6361 gtttcctcgc tgcaaaccgc cgccggaccg gtcgcgtacg cgcgcgtcgt ccacgccgcg
     6421 cactacgagc tggtcgggcc tccgacggca tccggcatgc gcgaggtcgt cgagcggctg
     6481 cgcgcgcacg cgcggttcac gatcgtcgac ggcgcgatcg atcgcgtggc cgcgacggcc
     6541 gggagtaccg gtgcgatcgt ggtggcggcc ggtgcttcgg ccgccggcac gcctgccgaa
     6601 gcggtcgatg cgattcgcgc gctggcgctg cggctcgggg tcgccggcta cgacgagaag
     6661 g
//
LOCUS       DASUCJ010001049         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_301403, whole genome shotgun sequence.
ACCESSION   DASUCJ010001049 DASUCJ010000000
VERSION     DASUCJ010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301403"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFU90_12415"
     CDS             <1..465
                     /locus_tag="VFU90_12415"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5480637.1"
                     /translation="TVTGVTSTGRMTWVRVIRGDIESPARRMETELEMFQRLADAAIS
                     IDQVTINQAGVAFAVGGDRGAEVRRLMGDLNLAVRVREGCSKLSVVGSGMRNTPGVVR
                     EVVEALSRENVEIIHCTDSNVTISILVPADDVARAERAVHEQFHLDAGDPPS"
     gene            462..1382
                     /gene="dapA"
                     /locus_tag="VFU90_12420"
     CDS             462..1382
                     /gene="dapA"
                     /locus_tag="VFU90_12420"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5480638.1"
                     /translation="MKLGTIVTAMITPFDRQGELDVNEAERLARWLIDRDTDGLVVAG
                     STGEGQTLDPGERRALWAAVKNAAGDRAAVIANAGTNATRESVAAVEEAAAAGADAIL
                     AVVPYYNKPTQTGMLKHFGAIAESTPLPVIVYNIPGRTGANMLPETLLELARSHANIV
                     GVKESSGDLKQIGMILRDRPRGFKVWSGDDHLFLPCLAMGADGVVGVATHLCSREYRR
                     MFEAYRSGDPVEAAEIHAAVLPLVDALFATTSPIPIKWAMKQLGFHTGDCRLPLDAIP
                     PALADRLRPLLARYASGATAKARGEAFAAR"
     gene            1401..2174
                     /locus_tag="VFU90_12425"
     CDS             1401..2174
                     /locus_tag="VFU90_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5480639.1"
                     /translation="MESVEILGCRLDAIGADEATSQILDYARRNAGAQVVTLGTEMVV
                     AAQKDERFRAIVNASALSLCDTVGLLTVARRRGAALKDRVTGVELTERLCAAAAEASQ
                     NGGDAIPVYFLGGASGVAADAAAILEVRFPGLDVAGVRDGYFREEESAEVAAAIKRSG
                     AKLLFVGMGSPRQEVWLAQHLRETGCGAGIGIGGTLDVLGGRVERAPYAFQRMGLEWL
                     YRLAQEPHRFRRQLALPHFVWLVTLEGAGLRHKKGMASS"
     gene            2180..>3178
                     /locus_tag="VFU90_12430"
     CDS             2180..>3178
                     /locus_tag="VFU90_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEU5480640.1"
                     /translation="MILAGGLSTRLYPLTRQVPKPLVPVAGVPNAEHVIHYLRAYGFD
                     EIAINLHYLADAIVDRLGDGSRFGVRLEYSYEPELLGSAGAVKKLEGFFGEENFVVVG
                     CDDLTDLPLDQLLAMHRERHAVATIGLVECDEVDQYGVVVLGERGKITGFQEKPAKGT
                     ERSKLANTGIYAFSPAIFSHVPAETFYDFGKQVFPALQSAGAPFFGFHARGAYWSDIG
                     TPSEYRRASFDAVRGAFTIPGARGSGVDPSARVEDGATLEGPVWVGADARIAAGARVL
                     GPSVIGDGVAIGPSVWVERSILWEGASVGEGAVLRDTIVGKGYRVEAGTTLENALVA"
BASE COUNT          524 a          995 c         1123 g          536 t
ORIGIN      
        1 accgtcaccg gggtgacgtc gaccggccgg atgacgtggg tgcgggtgat tcgcggcgac
       61 atcgaatcgc cggcccggcg catggaaacc gaactggaga tgttccaacg gctggccgac
      121 gccgcgattt cgatcgacca agtgacgatc aatcaagccg gcgtcgcgtt tgccgtcggc
      181 ggcgatcgcg gcgccgaagt ccgccgcctg atgggcgatt tgaaccttgc ggtccgggtg
      241 cgcgagggct gctcgaagct ctccgtcgtc ggcagcggaa tgcgcaatac gccgggcgtg
      301 gtgcgggaag tcgtggaggc gttgtcgcgc gaaaacgtgg agatcatcca ctgcaccgac
      361 agcaacgtga cgatctcgat tctggtgccc gccgacgacg tcgcgcgggc cgaacgggcc
      421 gtgcacgaac agtttcacct cgatgcagga gatccgccgt catgaagctc ggcaccatcg
      481 tcaccgccat gatcaccccg ttcgaccggc agggtgagct cgacgtcaac gaagccgaac
      541 ggctggcgcg ctggctgatc gaccgcgata ccgacggatt ggtggtcgcc ggttcgaccg
      601 gcgaagggca aacgctcgac cccggcgagc gccgtgccct gtgggcggcg gtaaagaacg
      661 cggccggcga tcgcgcggcg gttatcgcca acgccggcac caacgccacg cgcgaatcgg
      721 tcgcggccgt cgaagaagcg gccgccgccg gcgcggatgc gattttggcg gtcgttccgt
      781 attacaacaa acctacccaa accggcatgc tcaagcactt cggcgcgatc gccgaaagca
      841 cgccgctccc ggtgatcgtc tacaacattc ccggccgaac cggcgcgaac atgttgccgg
      901 aaacgctgct cgagctcgcg cgatcgcacg ccaacatcgt cggcgtgaaa gaatcgagcg
      961 gcgatctgaa gcagatcggc atgatcttgc gcgaccgtcc gcgcggattc aaggtatggt
     1021 ccggcgacga tcatttgttt ctgccgtgtt tggcgatggg cgccgacggc gtcgtcggtg
     1081 tggcgacgca tctgtgctcg cgcgaatacc ggcgcatgtt cgaggcgtat cgcagcggcg
     1141 atcccgtcga ggcggcggag atccacgcgg cggtgctgcc gctggtcgac gcgctgtttg
     1201 caacgacgag cccaattccg atcaagtggg cgatgaagca gctgggtttc cacaccggcg
     1261 actgccgctt gccgctcgac gccattccgc cggccctggc cgaccggctg cgcccgctgc
     1321 tcgcgcgcta cgcttcgggc gcgaccgcaa aggcgcgcgg cgaagcgttc gcggcgcgct
     1381 agcgaactcg gaatcggcga atggaatcgg tggaaattct cgggtgccgc ctcgacgcga
     1441 tcggcgcgga cgaggcgacg tcgcaaattc tcgactacgc gcgccgcaac gcgggcgcgc
     1501 aggtggtgac gctcggcacc gaaatggtgg tggcggcgca gaaggacgaa cgctttcgcg
     1561 caatcgtgaa cgcgagcgcg ctgtcgttgt gcgacaccgt cggtttgttg acggtagcgc
     1621 gccggcgcgg agccgcttta aaagatcggg tcaccggcgt cgagctgacc gagcggctgt
     1681 gtgcggcggc cgccgaggcg tcccaaaacg gcggcgacgc gatcccggtg tacttcttag
     1741 gcggagcctc tggggtcgct gccgacgcgg ccgcaatttt agaagtgcgc ttccccggat
     1801 tggacgtcgc cggcgtgcgc gacggctact tccgtgagga agagagcgcg gaggtcgcgg
     1861 cggcgataaa acgcagcgga gcaaagctgc tgttcgtggg gatgggatct ccacgtcaag
     1921 aggtgtggct cgcgcagcac ttgcgcgaaa ccggctgcgg ggccgggatc ggcatcggcg
     1981 gtaccttgga cgtgctgggc ggccgcgtcg aacgcgcccc ctacgcgttc cagcggatgg
     2041 gcctcgaatg gctctaccgt ctcgcgcagg agccgcatcg ctttcgccgt cagctcgcgc
     2101 tcccgcattt cgtttggctg gtgacgctcg agggtgccgg tttgcgtcat aagaagggga
     2161 tggcaagttc gtgaaggcga tgatcctggc gggcggcttg tcgacgcggc tgtatccgtt
     2221 aacgcggcaa gttcccaaac cgctcgttcc ggtggccggc gttcccaacg ccgagcacgt
     2281 aatccactac ttgcgcgcct acggcttcga cgagatcgcg atcaacctgc actatctcgc
     2341 cgatgcgatt gtcgaccggc tgggcgacgg cagccgtttc ggcgtgcggc tcgagtattc
     2401 gtacgagccc gagctgctcg gcagtgccgg cgcggtaaaa aaactggaag gttttttcgg
     2461 cgaggagaac ttcgtggtgg tcggctgcga cgatctcacc gacttgccgc tcgaccagct
     2521 cctcgcgatg catcgcgagc ggcacgccgt cgcgacgatc ggtttggtgg aatgcgacga
     2581 ggtcgatcag tacggcgtcg tcgtgctcgg ggagcgcggt aagatcaccg gcttccagga
     2641 gaagcctgcc aagggcaccg agcgcagcaa gctcgccaat accgggatct acgcgttttc
     2701 gcccgccatc ttttcgcacg tgcccgccga gacgttttac gatttcggca aacaggtctt
     2761 ccccgcgttg cagtccgccg gcgcaccgtt tttcggcttc cacgcgcgcg gcgcgtattg
     2821 gagcgatatc gggacgccgt cggagtaccg ccgcgccagc ttcgacgcgg ttcgcggcgc
     2881 ctttaccatt ccgggcgcgc gcggcagcgg cgtcgacccc agcgcgcggg tggaagacgg
     2941 cgcgacgctg gaaggcccgg tgtgggtcgg ggcggacgcg cgcatcgccg ccggcgcgcg
     3001 ggtgctgggg ccgagcgtca tcggtgacgg cgtcgccatc ggcccgagtg tgtgggtcga
     3061 gcgtagcatt ctttgggaag gcgccagcgt cggcgagggc gcggtgctgc gcgatacgat
     3121 cgtcgggaaa ggctaccgcg tggaagcggg gacgacactc gaaaatgcgc tggttgcc
//
LOCUS       DASUCJ010001050         1419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1524183, whole genome shotgun sequence.
ACCESSION   DASUCJ010001050 DASUCJ010000000
VERSION     DASUCJ010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524183"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1309
                     /locus_tag="VFU90_12435"
     CDS             92..1309
                     /locus_tag="VFU90_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480641.1"
                     /translation="MPAGTLTPIAGNPVAVDEAARALESVAAHVAATSSKLRALARTT
                     WTGSAATQASMRTATLPPKLDKVVASYSSAGAALRGYAVALADAQSRSVAALRAAASA
                     QADVAQLHAARATAPPEALPRYDASIADAEAALARAEASNEAAREDRCRAIGVAERRL
                     HDASSRGIRNQPWWRHILSSAARFAVSAWTTSLRLAASVATSISALAGLAALAVSIAG
                     LVFPPLEGAAAVLESISLVSGIMAAGADAALAASGNGSWKSVGIDAIALAPWAGSKLV
                     SKSARLLRAPKTTIYASSHVVEPAKVIAPVTSLATWGDVRTLARHFRDHGPDFGVTSP
                     TDYAARASQFLHDAIRDGELVKVDVDGIIRVYDRTANTFGSYNPDGSTRTFFKPTSAT
                     YWERQPGAKPWSE"
BASE COUNT          222 a          460 c          487 g          250 t
ORIGIN      
        1 tggatgcgga actacgccaa ggactgaggt tttccacagt cctcgcagct gctcgggcga
       61 gtgaatcgct gatttcacta gcgttttcgg cgtgcccgca ggaacgctga cgccgatcgc
      121 cggcaatccc gttgccgtag acgaagcggc gcgggcgttg gaatcggtgg ccgcgcacgt
      181 cgctgcgacg tcgtccaagc ttcgtgcgtt ggcgcggacg acgtggacgg ggagcgccgc
      241 gacgcaggcc agcatgcgca ccgcgacgct gccgccgaag ctggacaaag tcgtcgcgtc
      301 gtactcgtcg gccggcgcgg ccttgcgcgg ttacgcggtg gcgttggccg atgcgcagtc
      361 gcgatcggtt gccgcgctgc gtgctgccgc gtcggcgcaa gccgatgtcg cccagcttca
      421 cgccgcgcgc gccaccgcgc cgcctgaggc gctgccgcgc tacgacgcgt cgattgccga
      481 cgcggaggcg gcgctcgcgc gtgcggaggc ctcgaacgaa gcggcgcgcg aagaccggtg
      541 ccgcgcgatc ggcgtggcgg aacgccggct gcacgacgca tcttctcgag gaattcggaa
      601 ccagccgtgg tggcggcaca ttctcagcag cgctgcgcga ttcgccgttt cagcctggac
      661 gacgtcgctg cgcctcgcgg ctagcgtcgc gacgtccatc tcggcgctcg cgggtctcgc
      721 cgcattggcc gtgtcgatcg ccgggctggt ctttccgccg cttgaagggg ccgcggcggt
      781 actcgagtcg atctctttag tcagtggcat catggcggcg ggcgcggacg cggcgttggc
      841 ggcgtccggc aatggttcgt ggaagtcggt tggcatcgat gcgatcgcgc tggcgccgtg
      901 ggccggttcg aagctggtca gcaaatctgc ccgactgcta cgcgcgccga agacaacgat
      961 ctacgccagt tcgcatgttg tcgagccagc caaggtcatc gcgccagtca cgtcgctcgc
     1021 gacatggggt gacgtccgga ctctggcacg ccatttccgg gatcacggcc cggacttcgg
     1081 tgtcacgtcg ccaactgact atgccgcacg cgcgtcgcag ttcttgcacg acgcgattag
     1141 agacggagaa ctcgtgaagg tagatgtgga cggaatcatc cgcgtctacg accgcaccgc
     1201 aaacaccttt ggctcgtaca acccggatgg ctccacgcgc accttcttca aaccgacgag
     1261 tgccacatac tgggaacgtc aaccaggggc gaaaccgtgg agcgaatgat gtgcacctgc
     1321 cccgtatgcg ggtatcccgc acttgcagaa cctccgcgct cgaggtccgg cggagggtcg
     1381 tacgaaatct gcccctcgtg tggctttgag ttcggagtc
//
LOCUS       DASUCJ010001051         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1096397, whole genome shotgun sequence.
ACCESSION   DASUCJ010001051 DASUCJ010000000
VERSION     DASUCJ010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096397"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFU90_12440"
     CDS             <1..667
                     /locus_tag="VFU90_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480642.1"
                     /translation="YDHWTEPDRPRPGFGLTAFIPTRFPNGGDTQGLAADADGVFHAA
                     WINGATGTLQLWHTAFAVDSAIVAAVRNKNEQKMAGSAAAPVPSGRVDVTQELTFVMS
                     HPAIDFTRGTLEVTMRVKNPTGLTVHGPISVVVDRLETDWSHAMGLTNFRVANADSGG
                     RGVGATWVFAAGPERMLHPGATTPPRVLRFRFDGGVPDEPDGYFEPAFRILARSTSTA
                     GQR"
     gene            710..>1686
                     /locus_tag="VFU90_12445"
     CDS             710..>1686
                     /locus_tag="VFU90_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480643.1"
                     /translation="MHMHRMTAAAILCLTAMTPASRAQQVKPLSIQDALATRSFGIYA
                     PISVSADGRRVAYTIVDPRKRRSHSENAPNFLSTGAPRYAEGAELWIADIKTGATRDL
                     TGRTGNSWDGVWSPDGSRLAFYSDRDGAARLWVWDRATATVRRVSSVISRAWVMFESP
                     EWTPDGAALIVKVLPEGDTIGQSDDSIADATLALPNDSTPTAEVRTANIGRSDTAHVD
                     GGSNPALIADLALVRISDGTVRRIVRRSQFMGYWISPTGDRIAYTILSGMVVTPEVTF
                     VYDIGIADLRTNATRIIARRVPNFWGPTISWSPDGLHIAFTTKPHSYGL"
BASE COUNT          291 a          548 c          550 g          297 t
ORIGIN      
        1 gtatgaccac tggaccgagc cggatcgccc gcgtcccggg ttcgggctaa ccgcattcat
       61 cccgacgcgg tttcccaacg gcggcgacac ccagggactc gctgccgatg ccgacggcgt
      121 gttccacgcc gcgtggatca acggcgcgac ggggacgctt cagctttggc acaccgcctt
      181 cgcggtcgac tcggcgatcg tggccgcggt gcggaacaaa aacgagcaga agatggccgg
      241 ctcggcggcc gcgcccgtgc cgtcggggcg tgtggatgtc acgcaggaac tcaccttcgt
      301 catgagccac ccggcgatcg acttcacgcg cggcacgctc gaggtcacga tgcgggtgaa
      361 gaatccgacg ggcctcactg tgcatgggcc gatctcggtt gtcgtcgacc gcctggagac
      421 cgactggtcg catgcgatgg gcctaacgaa ctttcgcgtc gcgaacgccg acagcggtgg
      481 ccgcggcgtg ggtgcgacgt gggtgttcgc cgccggcccc gaacgcatgt tgcatcccgg
      541 cgccacgacg ccgccacgcg tgttgcgatt ccgctttgat ggcggcgtgc cggacgagcc
      601 ggatgggtat ttcgagcccg cgtttcgcat cctagcccgc agcacgagca ccgctggcca
      661 acgttaggct ggccgatcgc ccgtctcgca atattggagt gactccggca tgcacatgca
      721 tcgaatgacc gcggcggcaa ttttatgcct gaccgcgatg actcccgcgt ctcgcgcgca
      781 acaggtcaag cccttgtcca tccaggacgc gctggctacg cgctcgttcg ggatctatgc
      841 gccgatctcg gtgtcggctg atggacgccg ggtcgcgtac accatcgtgg atccacgaaa
      901 gcggcgaagt cacagtgaga atgcgcccaa ctttctctcg actggcgcgc cgcgctacgc
      961 cgagggcgcc gagctgtgga tcgccgacat caagacggga gccactcgag atctcaccgg
     1021 gcgcaccggg aatagctggg atggcgtgtg gtcgccggat ggatctcggc tcgctttcta
     1081 cagtgaccgt gacggagcgg cgcggctctg ggtgtgggac cgcgctacag ctacggtgcg
     1141 ccgcgtctcc tccgtgattt cgcgggcgtg ggtgatgttt gaatcgcccg aatggacgcc
     1201 cgatggggcg gcactgatcg tgaaagtgct tccggaaggc gacacgatcg ggcagtccga
     1261 cgacagcatt gccgacgcca cgctcgcgct gcctaacgat agcactccga cggcggaagt
     1321 ccgcacagcc aacatcggtc gttccgacac ggcgcacgtc gatggcggct cgaacccggc
     1381 actgatcgcg gaccttgcgc tcgtgcgaat ttcagacggc accgtgcggc gcatcgtccg
     1441 gcgatcgcaa ttcatgggat actggatttc gccgacaggc gaccggatcg cctatacgat
     1501 actcagtggc atggtggtca cgcccgaggt caccttcgtc tacgacatcg gcatcgccga
     1561 tctaaggacc aatgcgacac ggatcatcgc acggcgcgtt ccgaattttt ggggacccac
     1621 gattagctgg tcgccggatg gcttacatat cgcgttcacg accaagcccc acagctacgg
     1681 cctgca
//
LOCUS       DASUCJ010001052         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1065768, whole genome shotgun sequence.
ACCESSION   DASUCJ010001052 DASUCJ010000000
VERSION     DASUCJ010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065768"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFU90_12450"
     CDS             <1..763
                     /locus_tag="VFU90_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU5480644.1"
                     /translation="KALPPGSDETPRVYIFAGKAAAGYTTAKLHIRLINDVAAVINAD
                     PDTSAKLKIVFVPNYGVSLAQAIIPAIDVSLQISQAGKEASGTSNMKLALNGALTVGT
                     LDGANVEIRENVGTENFFLCGHTLEQVQELQRSGYDPKHFIAQSPPLAKAIAMLESGF
                     FSLGDGQRYLPVVSYLRDYDPYMVCADFDSFASEMSAAAALFRSPRPWTRRSLFNIVG
                     ASTFSSDATIRAYARDIWNLVPVKAKLRGSDGTSH"
     gene            911..>1014
                     /locus_tag="VFU90_12455"
     CDS             911..>1014
                     /locus_tag="VFU90_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5480645.1"
                     /translation="MLAKRVIPCLDVQNGRVVKGVQFLGLRDAGDPVE"
BASE COUNT          180 a          345 c          314 g          175 t
ORIGIN      
        1 gaaggctctg ccgcccggtt cggacgagac gccgcgcgtc tacatcttcg ccggtaaggc
       61 cgccgccgga tacacgacgg cgaagctgca catccggctg atcaacgatg tcgccgcggt
      121 gatcaatgcg gaccccgaca cgtccgcgaa gctcaagatc gtcttcgtcc cgaactacgg
      181 cgtctcgctc gcgcaggcga tcatcccggc gatcgacgtc tcgctgcaga tctcccaagc
      241 cggcaaagag gcgtccggca cgagcaacat gaagctggcg ctcaacggcg cgctcaccgt
      301 cggcaccctc gacggtgcga acgtggagat ccgcgaaaac gtcgggacgg agaatttctt
      361 tctctgcggc cacacgctcg agcaagtgca ggagctccag cgttcgggct acgacccgaa
      421 gcacttcatc gcccagagcc cccccctcgc caaggcgatc gcgatgctcg agagcggctt
      481 cttctcgctc ggcgacggcc agcggtatct gccggtcgtc agctacctgc gggactacga
      541 cccctacatg gtctgcgccg acttcgactc gttcgcgagc gagatgagcg ccgccgcggc
      601 gctcttccgg tcgccccgac cctggacgcg ccgatcgctc ttcaacatcg tcggtgcgag
      661 cacgttctcg agcgacgcga cgatacgcgc ctatgcgcgc gatatctgga acctcgtccc
      721 cgtcaaggcg aagctccgcg gatcggatgg gacgagccat tagatcagcg agcgcgattc
      781 tgcgaatgcg ctcgccgatt tttggccagg tttgttgcca aatcgccgtt cactgtcgtg
      841 acggccgatt tggcgcatcg ccgtggcacg agacatgacc ggcgcgacca cctaagctac
      901 gggtcgcact atgctggcaa agcgcgtcat tccgtgcctg gacgtgcaga atgggcgcgt
      961 cgtgaagggc gtgcagttcc tcggccttcg cgacgcgggc gaccccgtgg aatg
//
LOCUS       DASUCJ010001053         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1295059, whole genome shotgun sequence.
ACCESSION   DASUCJ010001053 DASUCJ010000000
VERSION     DASUCJ010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295059"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFU90_12460"
     CDS             complement(<1..734)
                     /locus_tag="VFU90_12460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5480646.1"
                     /translation="MLNGAVLGARAQVPGAPLPNAIGGRRWTMLPIPAPLIALVLSLH
                     AGCPANAARCVLKNVPPDSVGMSADRLAAISRVADRGLDAHGYPGLTVIVGRHGGLVW
                     EQGYGSVDWNPASPTADPLHTMYDLASLTKVIATTTAIMVLYDEHRIRLDDPVSRYLR
                     AYRHGAKRQVTIRMLLEHRAGLPPGRNVYRRGRPAAVARQMILATPLEYPPGEREVYS
                     DVGADILGFVVEAVSGQRLDAFVQAHV"
     gene            826..>1269
                     /locus_tag="VFU90_12465"
     CDS             826..>1269
                     /locus_tag="VFU90_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEU5480647.1"
                     /translation="MRLTFCLALAAVAGAGALPAQAGLVQENGTGRPVSYRRACQSPK
                     IEMIDAQLASGQESAALPATYLRHLDDAVFFYFSPVGWRTPRFAVAQFTLHRDGRITA
                     SRLVTPSGIADFDSALTNAITSAAATRAIAPIPAAVTADSLVVSLY"
BASE COUNT          209 a          439 c          430 g          191 t
ORIGIN      
        1 acgtgggcct gcacgaacgc atcgagccgc tgcccgctca ccgcctcgac cacgaagccg
       61 aggatgtcgg cgccgacgtc cgaatacacc tcgcgctcgc cgggcggata ctcgagcggc
      121 gtcgccagaa tcatctgccg cgcaacggcc gccggtcgcc cgcgtcgata cacgttgcgg
      181 cccggcggca gacccgcgcg atgctcgagc agcatccgga tcgtcacctg ccgtttggcg
      241 ccgtggcgat acgcacgcag gtacctggac accggatcgt cgaggcggat gcgatgctcg
      301 tcgtacagca ccatgatcgc cgtcgtcgtc gcgatcacct tggtgagcga cgcgagatcg
      361 tacatggtgt gcaacgggtc cgccgtgggg ctcgccggat tccaatcgac ggagccgtag
      421 ccctgttccc acacgaggcc gccgtgtcgc ccgacgatga cggtgagccc gggataaccg
      481 tgcgcgtcta acccgcggtc ggcgacgcgc gagatcgccg ccaagcgatc ggccgacatc
      541 ccgaccgaat cgggtggaac gttcttcaac acgcagcggg cggcgttcgc gggacagccg
      601 gcgtggagcg agaggacgag cgcgatgagc ggggcgggga tcggcagcat cgtccagcga
      661 cgaccgccga ttgcgttggg caacggcgcg ccggggactt gcgcgcgcgc gcctaaaaca
      721 gcaccgttga gcacgcccgc gcgccaacgg gcggcgcaaa tcctcgttgc gtgccgggcc
      781 cccggctctg gcgatgccaa tcttacagag tgatggagcg aaacgatgcg actcaccttc
      841 tgtctcgcgc ttgccgcggt cgccggcgcc ggcgcacttc ccgcgcaagc cggactggtc
      901 caggagaacg gcaccggccg gcccgtctcc taccgtaggg catgccagag tcccaagatc
      961 gagatgatcg atgcccagct ggcgagtggc caagagagcg ccgcgcttcc ggcgacgtat
     1021 ctgcgccacc tggacgacgc ggtctttttc tatttcagtc cggtcgggtg gcgcacgccg
     1081 cggttcgccg ttgcccaatt cacgctgcat cgcgacggcc gcatcacggc gagccggttg
     1141 gtcacgccat cgggcatcgc ggatttcgac agcgcgttga ccaacgcgat cacgtcggcg
     1201 gcggcgacgc gagcgatcgc cccgattcca gccgcggtca cagcagactc gctggtggtg
     1261 tcgctgtac
//
LOCUS       DASUCJ010001054         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_484972, whole genome shotgun sequence.
ACCESSION   DASUCJ010001054 DASUCJ010000000
VERSION     DASUCJ010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484972"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..921)
                     /locus_tag="VFU90_12470"
     CDS             complement(85..921)
                     /locus_tag="VFU90_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU5480648.1"
                     /translation="MRTLFAFAGYAMRGVRMRRLAVIMAGAAAVAAASTVALGCNRPG
                     SSGAAAGTAVADAGRRLTRDQVIAALARATDQRPANFTGDDLSGLDLSGVDFKEANLS
                     RARLVGTRFVHARMFGVTLSGAVAQGADFSNAVLDVSVMRGTDLSRASFRGASLYAAI
                     MIGANLTDADLTRARVIAALNNATLVRAKLDSANFGADRGNQPMGLMFTNLTDADLSG
                     ADLSHANLQKASFVRATLKGANLAGADVSYANFDQAIVTRVRGTNELIGLSTALHVPN
                     TW"
     gene            complement(969..1541)
                     /locus_tag="VFU90_12475"
     CDS             complement(969..1541)
                     /locus_tag="VFU90_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5480649.1"
                     /translation="MYGRRTVSRRAGGLCLFAVCLVAVMAFACSKDSATARASTATPA
                     GSTATVAAAKPGDTVMVVPPGTYCDKPNDSAEVWNEANHHRSDPLAVSQQEYDGWKMF
                     HVYCYRCHGTDALGSDIAPNLRHSLGPEGGVNKACFITTVWNGRPPKGMPTWKTLLDS
                     TQINNLYAYVTARSMGGLVPGRPHLGQVSK"
     gene            complement(1646..>2232)
                     /locus_tag="VFU90_12480"
     CDS             complement(1646..>2232)
                     /locus_tag="VFU90_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480650.1"
                     /translation="VRNTLGASECDVVMGVPAGYDPVLTTSPYYRSTYYIVTRADRHL
                     NIRSLDDPRLKSLKVGVGLIGDDYTNTPPAQALGSHGVTQNVRGFSLFYDEDTHPQDI
                     VNAVANGTIDVALVWGPVAGYFANASSVPLSLTALPDSDSASGVRFAYDVAIGVRHSD
                     REFKATLDSLLTEKRDTIQGILRAYHVPTLELKP"
BASE COUNT          371 a          792 c          684 g          385 t
ORIGIN      
        1 gtcgggcacg ccggccgctg cctcggcctc gacggcgcga ttcgctcgcg gtggccccat
       61 cgctggtggg ccaggctcgg ctgatcacca tgtgttaggc acgtggagcg ccgtcgacag
      121 gccgatgagc tcgttcgtgc cgcgcacccg tgtcacgatc gcctgatcga agttcgcgta
      181 cgagacatcg gcgccggcga gattcgcgcc cttcagcgtg gcccgcacga acgacgcttt
      241 ctgcaaattc gcatgagaca gatcggcgcc ggagagatcc gcgtcggtca gattggtgaa
      301 catgagcccc atcggctgat tgccgcggtc cgccccgaag ttcgcgctgt cgagcttcgc
      361 ccgcaccaac gtcgcgttgt tgagcgcggc gatcacacgc gcccgcgtga gatcggcgtc
      421 ggtgagattc gcgccgatca tgatcgccgc gtacaagctc gcaccgcgga acgacgcgcg
      481 gctgagatcg gtgccgcgca tcaccgatac gtccagcacg gcgttcgaga agtcggcacc
      541 ctgggccacc gccccgctca gcgtcacgcc gaacattctc gcgtgtacga agcgcgtccc
      601 cacgagccgc gcgcgactga ggttcgcctc tttgaagtcg acgcccgaca gatccagacc
      661 cgagagatca tcgcccgtaa aattcgccgg ccgctggtcc gtcgcccgcg cgagcgcggc
      721 gatcacttga tcgcgcgtca aacgccgccc cgcgtcggcg accgccgtcc ccgccgccgc
      781 gccggagctg ccgggccgat tgcagcctaa cgcaacggtc gatgccgcgg ccaccgcggc
      841 cgcaccggcc atgatcacag cgagtctccg catgcgcaca cctcgcatcg catacccggc
      901 aaacgcgaag agggtgcgca ccgcaccctc ttcccctcgg tccccgtccc cgtctcggac
      961 ccctggtctt acttcgatac ctgccccaga tggggccgcc cgggcaccaa cccgcccatg
     1021 ctgcgggccg tcacgtacgc gtacaggttg ttgatctggg tcgaatcgag gagcgttttc
     1081 cacgtcggca tgcccttcgg cggccgcccg ttccagacgg tcgtgatgaa gcacgccttg
     1141 ttcacgccgc cttccgggcc taacgaatga cgaagattgg gcgcgatgtc gctgcccaac
     1201 gcgtccgtcc cgtggcaccg gtagcagtac acgtggaaca tcttccagcc atcgtactcc
     1261 tgctgcgaca cggccagcgg atccgatcga tgatgattcg cctcgttcca cacttcggcc
     1321 gaatcgttag gcttgtcgca gtacgtcccc ggggggacca ccatcaccgt gtcccccggc
     1381 ttcgctgccg cgacggtcgc ggtcgacccc gcgggcgtcg cggtcgatgc ccgcgccgtt
     1441 gcgctgtcct tcgagcacgc gaaggccatc accgccacca gacacacggc aaagagacac
     1501 aggccgccgg cccggcggct gacggtacgc cggccataca tcggagagtg ttcgttcggc
     1561 aggttcattg attctgtatc gcgtcggcgt cgggttcgag tggtttcaac cgacttgagg
     1621 tgaatggttg gcccgaccgg ccgcctcagg gcttgagctc cagcgtcggc acgtgatagg
     1681 cccgcaggat cccttgaatc gtatcccgct tctccgttag caacgagtcg agcgttgcct
     1741 tgaactcgcg atccgagtga cgcaccccga tcgcaacgtc gtacgcaaac cgcacaccgc
     1801 tcgcgctgtc ggagtccggt agcgcggtca ggctcaacgg taccgatgac gcattggcga
     1861 aataccccgc caccggcccc cacaccagcg ccacgtcgat cgtaccgttc gcaaccgcgt
     1921 tcacgatgtc ctgcggatgg gtgtcctcgt cgtaaaacag actgaatccg cgcacgttct
     1981 gcgtcacgcc gtggctgccc aacgcctgcg ccggcggcgt gttcgtgtag tcgtcgccaa
     2041 tcagaccaac gccgactttc aatgacttga gccgcggatc gtccagcgac cggatgttca
     2101 aatggcgatc ggcgcgcgtc acgatgtagt acgtcgaccg gtagtacgga ctcgtggtca
     2161 gcaccgggtc gtatccggcc gggacgccca tcacgacgtc gcactcggat gcaccgagcg
     2221 tgttgcggac ga
//
LOCUS       DASUCJ010001055         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1172755, whole genome shotgun sequence.
ACCESSION   DASUCJ010001055 DASUCJ010000000
VERSION     DASUCJ010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172755"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..660)
                     /locus_tag="VFU90_12485"
     CDS             complement(13..660)
                     /locus_tag="VFU90_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU5480651.1"
                     /translation="MAHVDLIYYVDVLSSWCHVADYAVERIEAVYGERVRLDWRIAQL
                     FDYGPLPYSQNDLKWYYARTAKMTGVQLNESWHDAADTTTKFANQAAEAARALGAADS
                     RVRRGLSRAALLEGRKLGRRDEAVAYAARLSGFAPERIAEVMDSPQVTQRIAQTTCEF
                     KDLALPQLPSFVMRNTSGDLAVFSGLYTFEALDSVIGEMLHASRVTEEFGEVPAV"
     gene            complement(660..>1197)
                     /locus_tag="VFU90_12490"
     CDS             complement(660..>1197)
                     /locus_tag="VFU90_12490"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5480652.1"
                     /translation="LGMYDATAKIHASLKERALKPYSAEWNAAFDELLQEQANQPRNA
                     GRLWARIPQRRYVCFYPMDKKREGSDNWYMLPFEERAKLMHEHGKIGRLFHGLVTQVI
                     SGSVGYDDYEWGVDLYADDPLVFKKLIYEMRFDEASARYAGFGPFFTGIQFSVEDLGT
                     FLAGESVPELRAREEVHA"
BASE COUNT          221 a          382 c          362 g          232 t
ORIGIN      
        1 cgctggtaac ctctagaccg ccggtacttc gccgaattct tcggttacgc gcgaggcgtg
       61 cagcatctcg ccgatgacgc tatcgagcgc ctcgaaggta tacagccccg aaaacacggc
      121 caaatcgccg ctcgtgttgc gcatgacgaa cgacggcagc tgcggcaagg caagatcctt
      181 gaattcgcac gtcgtttgcg cgatacgctg cgtcacttgc ggcgaatcca tcacctccgc
      241 gatgcgctcg ggcgcaaatc cgctcaagcg ggcagcgtac gcgaccgctt catcgcgccg
      301 gccgagtttt cgtccctcga gcagtgcagc gcgcgaaagt ccgcgccgga ctctggaatc
      361 cgcggcgccg agagcgcgcg ccgcctcggc ggcttgattg gcgaacttcg tcgttgtgtc
      421 cgcggcgtcg tgccacgact cgttcagctg cacgccggtc atcttggccg ttcgcgcgta
      481 gtaccatttg agatcgttct gcgagtacgg cagcgggccg taatcgaaaa gctgcgcgat
      541 tcgccagtcc aaacgcacgc gctctccata caccgcttcg atacgctcga ccgcataatc
      601 ggcaacgtgg caccacgacg acagaacgtc gacgtagtaa atgaggtcga cgtgcgccat
      661 caggcgtgga cttcttcgcg ggcacgaagt tcgggcacgc tttcgccggc caggaacgtg
      721 ccgagatctt ccaccgagaa ctggatgccg gtaaagaacg ggccgaatcc cgcgtagcgc
      781 gcactggcct cgtcgaagcg catttcgtag atcagtttct tgaagacgag cggatcgtcg
      841 gcatagaggt cgacgcccca ttcatagtcg tcgtatccca cggagcccga gatcacttgc
      901 gtgacgaggc catggaatag ccgcccgatc ttgccgtgct cgtgcatgag cttcgcgcgc
      961 tcctcgaacg gaagcatgta ccagttgtcg ctgccttcgc gcttcttgtc catcggataa
     1021 aaacacacgt agcgccgctg cggaatacgc gcccagaggc ggccggcgtt gcgcggctgg
     1081 ttcgcctgtt cttgcagcag ttcgtcgaaa gccgcgttcc actcggcgga gtacggcttg
     1141 agcgcgcgct ctttgagcga cgcgtgaatc ttcgccgtgg cgtcgtacat gccgagt
//
LOCUS       DASUCJ010001056         1364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_57339, whole genome shotgun sequence.
ACCESSION   DASUCJ010001056 DASUCJ010000000
VERSION     DASUCJ010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57339"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFU90_12495"
     CDS             complement(<1..927)
                     /locus_tag="VFU90_12495"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU5480653.1"
                     /translation="MTIDEEVSRRRTFAIISHPDAGKTTLTEKLLLYGGAIHVAGQVS
                     ARKRQRQVTSDWMALERERGISITSTVLQFPYRGYVLNLLDTPGHQDFGEDTYRTLLA
                     ADSAVMLIDAAKGVEPQTKKLFSICRERGIPLFTFMNKLDRPSRDPLELLDELENVLG
                     IGAYPMNWPLGSGDSFSGVYDRRTHALHLFERSAHGATRALETITEIRDDASLRERVD
                     GYAFERFREGIELLDGAGEGFDRAAVLSGRVTPVFFGSAVTNFGVQLFLDNFTEMAPS
                     PSARAGIAPASGEFTGFVFKIQANMDPRHRDRI"
     gene            complement(924..>1364)
                     /locus_tag="VFU90_12500"
     CDS             complement(924..>1364)
                     /locus_tag="VFU90_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480654.1"
                     /translation="GWVNRDAIRLRIASVYAPVPPKPEAAGKFAAAAGGGVRGDAAWA
                     LSVFPECLSQVSRATGPREYVLAHLPKGAAAVTAPATLTYGDCTIVYSGSDVRVRRGT
                     DQLRIPPLVRLYRAPGALAVLHSDGSGGNDLRVYEPVRNPNSNQ"
BASE COUNT          235 a          465 c          435 g          229 t
ORIGIN      
        1 gatccggtcg cgatggcgcg gatccatgtt cgcctggatc ttgaaaacga agccggtaaa
       61 ctcgccgctc gccggcgcga tgcccgcccg cgcgctcggc gacggcgcca tctcggtaaa
      121 attgtccaga aacagctgca cgccgaaatt cgtcaccgcg ctcccaaaaa ataccggcgt
      181 gactcgtccg ctcagcacgg ccgcgcgatc gaagccttcg cccgcgccgt ccagaagctc
      241 gattccctcg cggaaacgct cgaaggcgta gccgtcgacg cgttcgcgga ggctcgcatc
      301 gtcgcgaatt tcggtaatcg tctccagcgc gcgcgtcgcg ccgtgcgccg atcgctcgaa
      361 gaggtgcagc gcgtgcgtgc ggcggtcgta caccccgctg aaggagtcgc cgctgcccag
      421 cggccagttc atcgggtacg ctccgatgcc cagaacgttc tccagctcgt cgagcagttc
      481 gagcggatcg cggctgggac ggtcgagctt gttcatgaag gtgaagagcg gaattccgcg
      541 ctcccggcaa atcgaaaaca gctttttggt ctgcggctcg acgcccttgg cagcgtcgat
      601 cagcatcacc gcgctatcgg ccgccagcag cgtgcggtag gtgtcctcgc cgaagtcctg
      661 gtgccccggg gtgtcgagca agttgagcac gtaaccgcga tacggaaact gcaagaccgt
      721 cgaggtaatc gaaatgccgc gctcgcgctc gagcgccatc caatcgctgg taacctggcg
      781 ctggcgtttc cgcgccgaca cttggccggc cacgtgaatc gcaccgccgt acagcagcag
      841 cttttcggta agcgtcgttt tgcccgcgtc gggatgcgag atgatcgcaa acgtccggcg
      901 ccgggatact tcttcgtcga tggtcattgg tttgagttcg ggttcctcac cggttcgtac
      961 acgcgcaggt cgttcccgcc cgaaccgtcc gaatggagaa ccgccaatgc ccccggcgct
     1021 cgatagagcc gaacgagcgg cggaattcgc agctggtcgg ttccgcgccg cacgcggacg
     1081 tcggaacccg aatatacgat cgtgcaatcc ccgtacgtca gggtggccgg cgccgttacg
     1141 gcggccgcgc ccttagggag gtgggccagc acgtattccc gcggcccggt cgcccgcgac
     1201 acttgcgaca agcattccgg gaacaccgag agcgcccagg ccgcgtcgcc gcgcacgccg
     1261 ccgccggcgg cggccgcgaa cttgcccgcc gcttcgggct tgggcggcac cggcgcataa
     1321 accgatgcga tccgcaagcg aatcgcgtcg cggttcaccc agcc
//
LOCUS       DASUCJ010001057         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_439296, whole genome shotgun sequence.
ACCESSION   DASUCJ010001057 DASUCJ010000000
VERSION     DASUCJ010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439296"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFU90_12505"
     CDS             <1..248
                     /locus_tag="VFU90_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480655.1"
                     /translation="VSMHGSITRDPGARPLANGLRVSSSGTVTGMLCVDRRRGWWIDA
                     RATIGLTSTLMPTATAAATPVRMQTDITQHMHTASVH"
     gene            297..>1227
                     /locus_tag="VFU90_12510"
     CDS             297..>1227
                     /locus_tag="VFU90_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5480656.1"
                     /translation="MRLVHLADLHLGFRQYHRLTPEGINQREADVALVFRRAMERVIA
                     LRPDVVLIVGDVFHSVRPMNAAIIEAFTQLQKLRLALPDTEVVIVAGNHDRPRATETG
                     SILELFTYIDEHVHVVAAGPRRVTLTAHDVSVLAVPDLPAGAPVPEFEPDRDARYNVV
                     AMHGEVEGMLPPNLAYPDRPSRPVPVETIGPARWTYVALGHYHVHKKMASNMYYCGSL
                     EYLPPNMWSEMHEERATGVPGKGFIEYDLDAGTHRFHAIAGARRLAELAPITARGLTA
                     ADVDAAIRERVASCEGGIDDRVVRLVLREIPRHV"
BASE COUNT          190 a          399 c          427 g          211 t
ORIGIN      
        1 tcgtctcgat gcacggcagc atcacgcgcg acccgggtgc ccgtccgctc gccaacggat
       61 tgcgggtgag ctcgtcgggt actgtgacgg gcatgttgtg cgtcgaccgg cgacgcggct
      121 ggtggatcga tgcccgggcg accatcgggc tcacgtcgac gctcatgccg accgccaccg
      181 ccgccgcgac gcccgtccgg atgcagacgg acatcacgca acacatgcac acagcgagcg
      241 tgcactgagc acgccgttcg gctagtcggc ccttcgccta agcggtagtt ttcggagtgc
      301 gcctcgtcca tctggccgac ttgcatctcg gcttccggca gtaccaccgg ctcacgcccg
      361 agggaatcaa ccagcgtgag gcggacgtcg cgctcgtgtt ccgtcgcgcg atggagcgcg
      421 tgatcgcgct caggccggat gtggtgctca tcgtgggcga cgtgttccac tcggtgcgtc
      481 cgatgaacgc cgcgatcatc gaagcgttca cgcaattgca gaagctccgg ctcgcgctgc
      541 cggacaccga ggtcgtgatc gtcgccggca atcacgatcg gccgcgtgcc acggagacgg
      601 gcagcatcct cgagctgttc acgtacatcg atgagcatgt gcatgtggtt gccgccgggc
      661 cgcgtcgtgt cacgctcacg gcgcacgatg tatcggtgct tgccgtgccg gatcttccgg
      721 cgggcgcgcc agtgccggag ttcgagcccg atcgcgacgc gcggtacaac gtcgtggcga
      781 tgcacggcga agtggaaggc atgcttccgc ctaacctggc ctatcccgac cggccgtcgc
      841 ggcccgtgcc ggtggagacg atcggtccgg cccggtggac gtacgtggcg ctggggcatt
      901 accacgtaca taaaaagatg gcctcgaata tgtactactg cggttcgctc gagtatctgc
      961 cgcccaacat gtggagcgag atgcacgaag agcgcgcgac cggtgtgccg gggaaggggt
     1021 tcatcgagta cgatctggac gcgggtacgc atcgcttcca cgccatcgcg ggcgcgcgcc
     1081 gcttggccga gctcgcgccg atcacggcgc gtggcctgac ggcggccgat gtggacgcgg
     1141 cgatccgcga gcgcgtcgca tcgtgcgagg gcgggatcga cgatcgggtc gtgcgcctcg
     1201 tgctgcgtga gattccgcgc cacgtgg
//
LOCUS       DASUCJ010001058         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_790556, whole genome shotgun sequence.
ACCESSION   DASUCJ010001058 DASUCJ010000000
VERSION     DASUCJ010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790556"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFU90_12515"
     CDS             complement(<1..794)
                     /locus_tag="VFU90_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5480657.1"
                     /translation="MTDRFRIALAQLNPVMGDIHGNLLMARAARAEATKAKADVVLFS
                     ELFIVGYPPEDLVLKPALQSDAREAIEKLAKDTADGGPAVLIGAPWVENDNLYNAFLF
                     LDGGKIAGRTFKYDLPNYGVFDEKRVFAAGPLPGPFNIRGVRVGVPICEDIWTSEVTE
                     CLAETGAEILAVPNGSPFEAGKEDVRANLIAARVTETKLPLIYLNQVGGQDELVFDGG
                     STVVNADCSTAIVMPSWQESLVTSEWSRRGATWVCATGERAASEERS"
     gene            890..>1243
                     /locus_tag="VFU90_12520"
     CDS             890..>1243
                     /locus_tag="VFU90_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480658.1"
                     /translation="MIGGVVDQECGLQSATRFLRTYQRRRTVPHRQDEGRDRFRQRIA
                     DFERPERPGRGVLDFARDEIDLDHAIAIFRAHAATPHLGDAAGFEMRGGAVRERERGR
                     NFVRQESVLRLSVRTD"
BASE COUNT          213 a          402 c          403 g          225 t
ORIGIN      
        1 gaacgttcct cgctcgccgc ccgctcgccg gtcgcgcaga cccaggtcgc tccgcgtcta
       61 ctccactcgc tggtcacgag gctttcctgc catgacggca tgacgatcgc cgtcgaacaa
      121 tcggcgttga cgacggtcga accgccgtcg aacaccagct cgtcttggcc gccgacctgg
      181 ttgagataga tgagcggcag ctttgtttcc gtcacgcgcg ccgcgatcag attggcgcgg
      241 acgtcttcct tgccggcctc gaatggcgag ccgttcggta ccgcgaggat ttcggcgccc
      301 gtctcggcca agcattcggt gacttcggac gtccagatgt cctcgcagat cggaacgccg
      361 acgcgcacgc cgcgaatgtt gaacggaccc ggcaagggtc ccgcggcgaa aacgcgcttt
      421 tcgtcgaaca cgccatagtt gggaagatcg tacttgaacg tgcggcccgc aatcttgccg
      481 ccatcgagaa agagaaaggc gttgtagaga ttatcgttct cgacccaggg tgctccgatg
      541 agcaccgccg gtccgccgtc ggcggtatcc ttggcgagct tttcgatcgc ttcgcgtgcg
      601 tcggactgca gtgccggctt cagaaccagg tcctccggcg gatagccgac gatgaaaagc
      661 tccgaaaata ggacgacgtc ggccttcgcc ttggttgcct cggcgcgcgc ggcgcgcgcc
      721 atcaggaggt tgccgtgaat atcgcccatc accgggttga gctgggcgag cgcgatgcga
      781 aaacggtccg tcatgcacgg tctttagcgc gcaattgcag ccagcaaaag gctgttcaac
      841 gggccgcggc gactgccacg tatgcctcgc gcgcacgggc atcgggccca tgatcggcgg
      901 cgtcgtcgat caagagtgcg gtctgcagag cgcgacgcgc ttcctgcgga cgtaccagcg
      961 gcggcgcacc gtgccgcacc gacaggacga aggccgcgac cgattccgcc aacggatcgc
     1021 cgatttcgag cggcctgagc ggcctgggcg tggtgttctt gactttgcgc gtgatgaaat
     1081 cgatctcgat cacgccatcg cgatattccg cgcgcatgcc gcgacgccgc acctcggaga
     1141 cgcggctggc ttcgagatgc gcggcggtgc cgttcgcgaa cgtgagcgag gccgaaactt
     1201 cgtccgccag gagtccgtgt tgcgcctgag cgtgcgcact gac
//
LOCUS       DASUCJ010001059         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_790568, whole genome shotgun sequence.
ACCESSION   DASUCJ010001059 DASUCJ010000000
VERSION     DASUCJ010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790568"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFU90_12525"
     CDS             complement(<1..380)
                     /locus_tag="VFU90_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU5480659.1"
                     /translation="MGATLASTMLGFLREVVSAKYYGTRWEMDTFLAAATIPTILFGV
                     FNGALVSALVPTFSEYLAHRDETEAWRLGSTVLNLLAIVLTACAVIGFFTARWYVPLI
                     AHGFPAPQIGVAIRMTRWLMPSIVA"
     gene            complement(460..1104)
                     /locus_tag="VFU90_12530"
     CDS             complement(460..1104)
                     /locus_tag="VFU90_12530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5480660.1"
                     /translation="MQAAIAPPAERVVPESWRALKRTIDFSLGVLLLIAVAPILLVAA
                     IGIVCTTGGSPFFAQERVGMNGRRFKMFKLRTMVNGAHDMRDGLMHLNEVDGPVFKIR
                     NDPRLHPLGRFLRRTSIDELPNLLNVVLGDMSLVGPRPALPCEVAHYDGFAMRRLTVP
                     QGLTCLWQINGRSQVSFEEWMVLDNHYVDTWSPIGDLVLIVQTVPAVLRKDGAH"
     gene            1681..>1956
                     /locus_tag="VFU90_12535"
     CDS             1681..>1956
                     /locus_tag="VFU90_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480661.1"
                     /translation="MRRIPLCAALALLAFATACASARANTIVRLTADRIDFYYDRFLI
                     EADGHVRLQTSDGFSAEGDAFSMDLKLNRYLLAGNVTLRANGQQASGA"
BASE COUNT          362 a          654 c          618 g          322 t
ORIGIN      
        1 gcgacgatgc tcggcatcag ccagcgcgtc attcggatcg cgacgccgat ttggggggcc
       61 ggaaatccgt gtgcgatcag cggcacgtac cagcgggcgg taaaaaatcc gatcaccgcg
      121 cacgcggtga gcacgatcgc cagcaggttg agcaccgtgc tgcccagacg ccaagcttcg
      181 gtttcgtcgc ggtgggcgag atattcggag aacgtcggga cgagcgcgct caccagcgca
      241 ccgttaaaca ccccgaagag gatcgtgggg atcgtcgccg ccgccaggaa ggtatccatt
      301 tcccagcgcg ttccgtaata tttggcgctg acgacctcgc gcaagaatcc gagcatcgtc
      361 gatgccagcg tcgcgcccat gatgaaaagc gtcgacccgg cgatggagaa gcggcgtggg
      421 tgcggacgtg cggcggcgga gacgacccgg aggtgttgcc taatgcgcgc catccttccg
      481 caagaccgcc ggcacggtct gcacgatcaa aaccagatcg ccgatcggcg accaggtatc
      541 gacgtagtgg ttgtcgagga ccatccactc ctcgaacgag acttggctgc ggccgttgat
      601 ctgccacagg cacgtaagcc cttgtggaac ggttaaccgg cgcatcgcaa agccgtcgta
      661 atgcgccacc tcgcacggca gtgccggccg cgggccgacc agcgacatgt cgccgagaac
      721 gacgttgagc aggttgggca gctcgtcgat gctggtccgt cgcaggaacc ggccgagtgg
      781 atgcagccgc ggatcgtttc ggatcttgaa taccggcccg tccacttcgt tcaagtgcat
      841 cagtccgtcg cgcatgtcgt gcgcgccgtt gaccatcgtc cgaagcttga acattttgaa
      901 gcgccggccg ttcatgccga cgcgttcctg agcgaaaaac ggcgagccgc cggtcgtgca
      961 gacgatgccg atcgccgcga cgagcaagat cggtgcgacc gcgatcagca gcaacacacc
     1021 gagagagaaa tcgatcgtgc gtttgagcgc gcgccacgac tcggggacga cgcgttcggc
     1081 cggaggtgca atcgccgcct gcacgggtta cgcgctcgat cctagccggt tcgcgggcgc
     1141 gcgcgcaatc cgaatgcgcg gcaccgaatt atcaccgaaa atctcgctca tctcgtcaga
     1201 cgcacgcacc tcgacacccc acgcaacgag ttcaccgagc gggtccttgc gccgcccgac
     1261 tgcatagccg catcgttcgg cgcttggtaa aaagaccccg gtcgaccgtc gccttggcga
     1321 atcgtaccag ccgcggagag gattcacctc cccgggagcc gccggcaccg ctagtccgat
     1381 tctcccgaag gccgccgccg ccggccggcc gcgcgcaccc gcgcgacccg gcaacccaag
     1441 cgcacggcgc gagcgcgccg tcgtttccga ggccgagcgt accctcgctc gacgctgcga
     1501 cggcgtcgcg gcgacgcgcg gcgattgcac gcgcgccgca ccgaaatcga ccgcgatcgt
     1561 gtctgcgcgc acggctccgc aattcggctt atttgcttgc gcgcctgctg cgcgttttgc
     1621 ataggatacc gcacgcctcg gtcaaaacgg tcccgagact taccaccctc cgaggaacac
     1681 gtgcgacgga ttccgctctg cgcagctctc gcgctgctgg cgttcgcgac ggcttgcgcg
     1741 tcggcgcgag cgaatacgat cgtgcgcttg acggccgatc gcatcgattt ttactacgat
     1801 cgatttctga tcgaagccga cggccacgtc cgtttgcaaa cgagcgacgg cttctctgcc
     1861 gaaggcgacg ccttttcgat ggacctcaag ctcaatcggt atttactggc cgggaacgtc
     1921 acgctgcgcg cgaacggcca gcaggcgtcc ggcgcc
//
LOCUS       DASUCJ010001060         1374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1218845, whole genome shotgun sequence.
ACCESSION   DASUCJ010001060 DASUCJ010000000
VERSION     DASUCJ010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218845"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFU90_12540"
     CDS             <1..458
                     /locus_tag="VFU90_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480662.1"
                     /translation="AAFDPPGLLGRRIGPMFVAVGGRRDRVDAVDIQFTADVLAHMAE
                     RFNEAPNVVNLIATDPPTKRDLVGNLRRDNPGLTVIWLPWPVLVPLSGMATLLQKALR
                     PRRPAISLRKVFASRRYDNAQVRALAARMGDEKSATASGGNANRGTAYP"
     gene            455..>1374
                     /locus_tag="VFU90_12545"
     CDS             455..>1374
                     /locus_tag="VFU90_12545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5480663.1"
                     /translation="MTRPKLLVLSQTLPFPPDGGVKVRTYNLLKQLAIAFDIVALCFY
                     RSKTSSDSAVAGLRGLARVEAFPIPQEHSRLRLLRDHLHSSVSRRVYTAFAYRSHKFR
                     ARLVDLLASEHFALAHADSLDLSDYFPLVGDVPLICVHHDVQSALLRRRAQQQRAPWM
                     RGYIAYQAELMRREERRWCPRVDLNVTVSDVDRDALRAVAPGSRVAVVPNGVDVDYFR
                     PQGGGAANTVVFVGGSSWFPNADAMRHFGQAILPLIRERGASPHVVWVGSTTAEERSG
                     FRRDFGIEATGYVEDTRPHVGGAGCCIAPIR"
BASE COUNT          219 a          456 c          448 g          251 t
ORIGIN      
        1 acgcggcgtt cgatcctccg gggctgctcg gccggcgcat cggcccgatg ttcgtggccg
       61 tcggcggacg acgcgaccgt gtcgacgccg tcgacatcca attcacagcc gatgtcctgg
      121 cccatatggc cgagcggttc aacgaggcgc cgaacgtcgt caacctgatc gcaacggacc
      181 cgcccacgaa acgggacctc gtcggaaacc tgcgtcggga caaccccggc ctcaccgtca
      241 tctggctccc gtggccggtt cttgtgccgc tctcgggcat ggccacgctg ctacagaagg
      301 cactgcggcc gcgacgccca gcgatcagct tgcgcaaggt ctttgcttcg cggcgctacg
      361 acaacgcgca agtccgcgcc ttggcggcgc gcatgggtga cgagaagtct gcaaccgcga
      421 gcggcggaaa cgcgaatcgg ggcacggcgt atccgtgacg cggccgaagc ttctcgttct
      481 gtcgcaaacg ctgccgttcc cgcccgacgg cggagtcaag gttcgcacgt acaacctcct
      541 caagcagctc gcgatcgcgt tcgatatcgt cgcgctttgc ttctatcgct cgaagaccag
      601 cagcgactcg gcggtcgcgg ggcttcgagg cttggcgcgt gtggaagcat tcccgattcc
      661 acaggagcat tcgcggctgc gcttgctgcg cgaccatctt catagctcgg tgagccggcg
      721 cgtctacacc gcgttcgcgt accgttcgca caaattccgc gcgcgtctcg tcgaccttct
      781 cgcctccgag cacttcgcgc ttgctcatgc ggacagtctc gacctgtccg actatttccc
      841 gctcgtcggc gacgtgccgc tgatctgcgt ccatcacgat gtgcaatcgg cgttgctcag
      901 gagacgcgcc caacaacagc gcgcgccgtg gatgcgcggc tacatcgcgt atcaggccga
      961 gttgatgagg cgcgaggaac gtcgctggtg cccgcgtgtc gatttgaacg tgacggtgtc
     1021 ggacgtcgac cgcgatgcgc ttcgcgccgt ggcgcctggg tcgcgtgtcg ccgttgtccc
     1081 gaatggagta gacgtggact acttccgccc gcagggcggc ggagctgcca acaccgtcgt
     1141 ttttgtcggc ggcagcagct ggtttccgaa cgccgacgcc atgcggcact ttggccaagc
     1201 gattctcccg ttgattcggg agcggggcgc atcaccgcac gtcgtatggg tcggaagcac
     1261 gacggcggag gagagaagcg gattccggcg cgacttcggc atcgaggcaa ccggctacgt
     1321 cgaagacacg cggccgcatg tcggaggggc agggtgctgc atcgctccga ttcg
//
LOCUS       DASUCJ010001061         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_622335, whole genome shotgun sequence.
ACCESSION   DASUCJ010001061 DASUCJ010000000
VERSION     DASUCJ010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622335"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VFU90_12550"
     CDS             complement(<1..1745)
                     /locus_tag="VFU90_12550"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5480664.1"
                     /translation="MLADTVTIDGNLSEPVWSKAARLTDFSEYLPVDGQPAADSTEVL
                     VWYSADAIYFGIRAYELHGPVHATLADRDNISSDDYVQIFLDTFDDHRRAYVFGANPL
                     GVQADGILSEGIQSHAAGIGSAGTTRDTVDLSTDYTYDSRGRVLPDGYEVEIRVPFKA
                     IRIQAGREQRWGLNILRHVQHSGHVSTWAPARQANASFLAQSGSLVGLTGLERGHALE
                     INPELTSHVDGAPRGAGWHYGSPIQQLGGNLRYALTDNLTADATVKPDFSQIESDVPE
                     LSYDPRDALSYPEKRPFFLDGLEQFDTPTSLIYTRSILQPVAAAKLTGTISGTDIGFL
                     SAIDNSAASETGTVNPVFNILRVRRGIGPNVSLGTVLTDREEAGRYNRVGGLDARILF
                     DEIYSVRLQGAASTTRGDSGTIGGPLWGATFERQGHTIQLHYSMSGIDPDFRTQSGFV
                     SRSNVAAVTGEQAITLHGARGSLIESWTGDFTANHSWIYTRFARGKSPDDLRWHISNQ
                     LTLRGGWVLVGNVFLERYGYDPSIYTNYYIVHPTIGGADTTKFLGTPHIPNLDIVAGI
                     TTPRWKNFDASIQTIT"
BASE COUNT          320 a          582 c          635 g          333 t
ORIGIN      
        1 gtgatggtct ggatgctggc gtcgaagttt ttccagcgcg gcgtcgtgat gccggccacg
       61 atgtccagat tcggaatgtg cggcgtcccc agaaacttcg tcgtgtccgc accgccgatg
      121 gtgggatgca cgatgtagta gttcgtgtag atcgacggat cgtatccgta ccgctcgagg
      181 aagacgttgc ccaccagcac ccatccgccg cgcagcgtca actggttgct gatgtgccag
      241 cgcaagtcat cgggactctt gccgcgcgcg aatcgcgtgt agatccagct gtggttggcg
      301 gtgaaatcgc cggtccaact ttcgatgagg ctgccgcgcg caccgtgcag cgtgatggcc
      361 tgctccccgg tcaccgccgc gacgttggag cgcgacacga atccgctctg cgtgcggaaa
      421 tccgggtcga tgcccgacat cgaatagtgc agctggatcg tgtggccctg gcgctcgaac
      481 gtcgcgcccc acagcggtcc gccgatcgtc cccgaatcgc cgcgcgtggt gctcgccgcg
      541 ccctggagac gcacgctgta gatctcgtcg aacagaatcc tcgcgtcgag cccgccgacc
      601 cgattgtagc ggccggcttc ttctcggtcg gtgagcacgg tgcctaacga cacgttgggc
      661 ccgatgccgc gtcgcacgcg caggatgttg aacaccgggt tgacggtgcc ggtctccgac
      721 gcggccgaat tgtcgatagc cgacaggaag ccgatgtccg tgccggatat cgtgccggtc
      781 agcttggccg ccgccaccgg ctggagaatg ctgcgcgtgt agatgagcga ggtgggcgtg
      841 tcgaactgct cgaggccgtc caggaagaac ggacgctttt cgggatacga gagcgcgtcg
      901 cgcggatcgt acgacagctc gggtacgtcg ctctcgatct gcgagaagtc cggcttgacc
      961 gtggcgtccg cagtgagatt gtcggtgagg gcgtagcgca gattgccgcc cagctgttga
     1021 atgggcgagc cgtaatgcca gccggcgccc cgcggcgcgc catcgacgtg cgacgtgagc
     1081 tcgggattga tctccagggc gtgcccgcgc tcgaggcccg ttaggccgac gagcgagccc
     1141 gattgcgcga ggaacgacgc attcgcctgc cgcgccggcg cccacgtgct cacgtgtccc
     1201 gagtgctgca cgtggcgcag aatgttgagc ccccaccgct gctcgcgtcc cgcctgaatc
     1261 cggatcgcct tgaacggcac gcggatctcg acctcgtagc catccggcag cacgcgtccg
     1321 cgcgagtcgt acgtgtaatc ggtgctcaga tccacggtat cgcgcgtggt gccggcgctg
     1381 ccgatgccgg cggcgtgcga ctgaattcct tcgctcagga tgccgtcggc ctgcacgccc
     1441 aacggattgg cgccgaagac gtacgcgcga cgatgatcgt cgaacgtgtc gagaaagatc
     1501 tggacgtagt cgtcggacga gatgttgtcg cgatcggcaa gcgtcgcgtg cacgggcccg
     1561 tggagctcgt acgcccggat gccgaagtaa atcgcgtcgg ccgagtacca gacgagcact
     1621 tcagtggaat cggcggcggg ttggccgtcg acggggagat actcggaaaa atcggtgagc
     1681 cgtgcggcct ttgaccacac gggctcgctg agattcccgt cgatggtgac cgtgtccgcc
     1741 agcatgggcg cacgcacgtc gacattccca ctgcgtccac tgaatacgag cccagcagaa
     1801 gccgcggcca cctgaaacag cgcgataatg gggagcagga tgcttcagct cgattgcagg
     1861 aagggtgaga
//
LOCUS       DASUCJ010001062         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1310191, whole genome shotgun sequence.
ACCESSION   DASUCJ010001062 DASUCJ010000000
VERSION     DASUCJ010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310191"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFU90_12555"
     CDS             <1..481
                     /locus_tag="VFU90_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5480665.1"
                     /translation="AGGVDVTAPDPLAELLVRVPDGVTLAVDARAGDVHVTDITGNAR
                     IATGTGDVDVMLPGYAQARTGRGNVSIRMGSVDWPGTLHIATGRGDVEVWIRDKASFH
                     AHLHTDSGMIFTDFNLRGTSQGGAETIDGDVNGGGGHGVEIDTGAGAIRLLQLHPQP"
     gene            complement(503..829)
                     /locus_tag="VFU90_12560"
     CDS             complement(503..829)
                     /locus_tag="VFU90_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480666.1"
                     /translation="MTPGYNLVLFAVDSLQAAIAQDLSSRYCPHSKALFLMRFWALFA
                     VVAAAGLTAFFAAIRQRNRTPDRTSPYVIAGTVLLFAIGSGALVLYGLSGCSGAAAEG
                     LIWDWP"
     gene            complement(833..1330)
                     /locus_tag="VFU90_12565"
     CDS             complement(833..1330)
                     /locus_tag="VFU90_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480667.1"
                     /translation="MDVLWGLGARYCVRQDAHVFMWLWGLLLTLAATGFVVRRRGRPF
                     EAAVLLFLTLCTGALGLVIFSACYSTAPYQMGLGALNISARTLSALTCLSLGAFTLAF
                     LWTVVALSLGGALGLVAWGRSDGTSAALRASLYIGAACLLVVAAGAGFIGSFNFSWCA
                     SQRLF"
     gene            1488..2516
                     /locus_tag="VFU90_12570"
     CDS             1488..2516
                     /locus_tag="VFU90_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480668.1"
                     /translation="MKMAALGRYSAAAGAMAVCASLLAACSVRSNLDALPNTSPAGGL
                     DAVAHKRGTVKTFNDPFGDPAPAGITAGPDGALWFTDPGNDVIGRITTDGTYTLQTSA
                     GVEISDGIVTGPDGNLWFTVAQSNAFIGRITPAGAVKLFQDPGGNFPHGITAGPDGAL
                     WFAESNGTVGRMTTKGSVKHFPVGASNAQLEGIVTGPDRRLWVTQYVVGGSHLSNRVI
                     RLTTTGKSKPFAVGSGPQSICVGPDKALWFTETGASALGRLTTSGSFREFPIGDPSAN
                     ASGIAAGPDGALWFTDDKGIGRMTTGGRVRVFAASGSLPQITAGPDGAMWFTSSFAPP
                     AIGRITTN"
     gene            complement(2503..3039)
                     /locus_tag="VFU90_12575"
     CDS             complement(2503..3039)
                     /locus_tag="VFU90_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5480669.1"
                     /translation="MPAPADEARARARLELVREHIGCENRRDLDGIMRTFGAAAEYDD
                     LPWDDRRTGRDGVQAYYAELLQSLPDLQIDVRGAHACADTVVVEAVIRGTHRGAWRGL
                     PATGRALAFPLCGIFTFDGGGRIAGERIYYDRATVLRQLGVFHEPQTPLGAVLTALTH
                     PVTIARALAAKLRIASSS"
BASE COUNT          604 a         1050 c         1036 g          455 t
ORIGIN      
        1 cgccggcggc gtcgacgtca ccgcacccga tccgctagca gagctcctcg tgcgcgtccc
       61 cgacggggta acgctggccg tcgacgcgcg cgccggcgac gtgcacgtca ccgacatcac
      121 cgggaacgcg cgcatcgcga ccgggacggg cgacgtggac gtgatgctgc cgggctacgc
      181 gcaggcgcga acgggccgcg ggaacgtctc gatccgcatg ggttcggtcg attggcccgg
      241 cacgctgcac atcgcgaccg gtcgcggcga cgtcgaagtt tggattcgcg ataaagcctc
      301 gttccacgcc catctgcata ccgatagcgg catgattttc accgatttca acctgcgcgg
      361 cacctcgcag ggcggtgcgg aaacgatcga cggcgacgtc aacggcggcg gcggccacgg
      421 cgtcgagatc gataccggcg ccggcgcgat ccgcctgctg cagctgcacc cgcaaccgta
      481 gcctcgtcgc atcggcgatc gtctacggcc aatcccaaat aaggccttcg gccgccgcgc
      541 cgctgcaacc ggagagtccg taaagaacca acgcgccgct gccgatcgca aagagcaaga
      601 cggtgccggc aatcacgtac ggcgaagttc gatccggcgt gcggttccgt tggcgaatcg
      661 cggcgaagaa cgccgtcaga ccggcggcgg cgacgaccgc aaagagcgcc caaaaccgca
      721 tcaagaacag ggctttactg tgcggacaat agcgcgacga cagatcctgc gcgatggccg
      781 cctgcaagct atcgaccgcg aagagaacga ggttgtatcc gggtgtcatg gctcaaaaca
      841 accgctgcga cgcgcaccaa gagaaattga aggatccaat gaagccggcg ccggccgcga
      901 cgaccagcaa gcacgcggca ccgatgtaga gactcgcacg aagagccgca ctcgtgccgt
      961 ccgatcggcc ccaggcgacc agcccgaggg cgccgccgag cgacagcgcg acgaccgtcc
     1021 ataggaaagc caacgtaaat gcacctaggc tcaggcaggt gagggccgag agggtcctcg
     1081 ccgagatgtt gagcgcaccg agacccatct ggtacggcgc ggtcgagtaa caggcggaga
     1141 aaataacgag ccccagcgcg ccggtgcaga gcgtgagaaa cagcagcacc gccgcctcga
     1201 acggccttcc gcgccggcgt acgacgaatc ccgtggcggc aagtgtcagc aacagacccc
     1261 acagccacat gaagacgtgg gcgtcttgcc gtacgcagta gcgggctccc agaccccata
     1321 gcacgtccac caggtgaggg tatcacgata cgcgttccag cgtgaacccg gaggcagtca
     1381 cgtcgcctta gctccaaacg taaacgtcta tgggcgaccc gcgcctgcgg cctcgcttcg
     1441 gccgcgccgc gattgagagc gggcgaagac gaaaggagag agaccacatg aagatggccg
     1501 cgcttggccg atactcggcc gccgccggcg cgatggcggt ttgcgcatcc ctattggcgg
     1561 cgtgctcggt tcgttcgaac ctcgacgcac tgccgaacac ctcgcccgcc ggcgggctcg
     1621 acgccgtcgc gcacaagcgc ggcacggtga agactttcaa cgatccgttc ggcgacccgg
     1681 caccggccgg gattacggcc ggacccgatg gcgcgctgtg gtttaccgat ccgggcaacg
     1741 acgtcatcgg acgcatcacg acggacggaa cctacacgtt gcagacgagc gcgggcgttg
     1801 aaatctccga cggtatcgtg acggggcccg acgggaatct gtggtttacg gtcgcgcaat
     1861 cgaacgcttt catcggccgc atcacccccg ccggggccgt gaaactcttt caggatcccg
     1921 gcggaaactt tccccacggc attacggccg gacccgacgg ggcgctctgg tttgccgaat
     1981 cgaacggcac cgtcggccgg atgacgacga agggaagcgt caaacacttc ccagttgggg
     2041 cgagcaacgc gcagctcgag ggcatcgtga ccggccccga ccggcggctg tgggtaacgc
     2101 aatacgtcgt cggcggcagc catctatcga atcgagtcat tcgccttacg acgaccggaa
     2161 aaagtaagcc gttcgcggta ggatcgggtc ctcagtctat ttgcgtcggc cccgacaagg
     2221 cgctgtggtt tacggaaacg ggagcgtcgg cgctcgggag gctgacgacc agcggatcgt
     2281 tccgagaatt tccgatcggc gacccctcgg ccaacgcctc ggggatcgcg gcgggaccgg
     2341 atggagcctt atggttcacc gacgacaaag gcatcggccg catgactacc ggcggaagag
     2401 taagggtctt cgccgcgtcc ggatcgcttc cgcagatcac cgccgggccg gacggcgcaa
     2461 tgtggtttac gagttcgttc gctcctccgg caatcgggcg catcacgacg aactagcgat
     2521 gcggagcttc gcagccaacg cccgggcgat cgtcaccgga tgggtcagag cggtgagaac
     2581 ggcgccgagc ggcgtttgag gttcgtggaa gacgccgagc tgccgcagaa cggtcgcgcg
     2641 gtcgtagtag atacgctcgc ccgcgatccg gccgccgccg tcgaacgtaa atatcccgca
     2701 cagcggaaac gcgagtgcgc gaccggtcgc gggcagcccc cgccacgcgc cgcgatgggt
     2761 tccgcggatg acggcttcca ccacgacggt gtcggcgcac gcatgcgcgc cgcgcacgtc
     2821 gatctgcaaa tcggggaggc tttgcagcag ctccgcgtag tacgcctgca cgccgtcgcg
     2881 tccggttcgc cggtcgtccc acggcagatc gtcgtattcg gcggccgcgc cgaacgtgcg
     2941 catgatgccg tcgagatcgc gccggttctc gcagccgatg tgctcgcgaa cgagctccag
     3001 ccgcgctcga gcccgagctt catcggccgg cgccggcatg cgccctcgcc aacagcgctt
     3061 cgcgcgcttt gatcaagcgc accattgcat cgttatacgg cgtcggcgtt ccggtgcgcc
     3121 gcccgaaggc gaccaccgcg ccgtt
//
LOCUS       DASUCJ010001063         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1065921, whole genome shotgun sequence.
ACCESSION   DASUCJ010001063 DASUCJ010000000
VERSION     DASUCJ010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065921"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1004
                     /locus_tag="VFU90_12580"
     CDS             91..>1004
                     /locus_tag="VFU90_12580"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5480670.1"
                     /translation="MHTFTKPSLTAGEKTLPASWYVSPEIYAHERERVFAREWICVGR
                     SERLENAGDFFTVEPAGESLIVTRDRAGTVHAFFNVYRHRGTRICELSSGRFQGSIQC
                     SYHAWTYGLDGALKVARNMAEVPGFDVRAYPLREAAVALWEGFIFVNLAAEPEPFEIA
                     LAPLAGRFSKWDVENLRTARTIEYDVACNWKLVFQNYSECYHCPLVHPQLDKLSPSDS
                     GRNDLTAGPVLGGFSELRVHGASLTTTGQTLRPPLGGVDGDDLDRVYYYTIFPSMLLS
                     LHPDYVMVHYVEPTGIARTRVTCSWLFDP"
BASE COUNT          178 a          330 c          310 g          186 t
ORIGIN      
        1 tacaggggat tccggcaaac gcggtggaat ccttggaata gctcgctcac ggagatggcg
       61 cagttgcatc attgggaggt ccgcgcaacg atgcacacgt ttacgaaacc gtccctcacc
      121 gccggggaaa agacgcttcc cgcgtcgtgg tacgtctcgc ccgagatcta cgcgcacgag
      181 cgcgagcgcg tgtttgcgcg cgaatggatt tgcgtaggac gcagcgagcg cctcgaaaac
      241 gccggcgact tcttcaccgt cgaaccggcg ggtgaaagtt tgatcgtcac gcgcgaccgc
      301 gccggcaccg tgcacgcctt cttcaacgtc taccggcacc gcgggacgcg catctgcgag
      361 ctgtcgtcgg gacgctttca aggctcgatt cagtgctcgt accacgcgtg gacgtatggg
      421 ctcgacgggg cgctgaaagt cgcccgcaac atggccgaag tccccggatt cgacgtgcga
      481 gcttacccgt tgcgcgaggc ggccgtcgcg ctgtgggaag gtttcatctt cgtcaacctg
      541 gcggcggaac cggaaccgtt cgagattgcg ctcgcgccgc tggccggccg cttttcgaag
      601 tgggacgtcg aaaatctgcg gaccgcccgc acgatcgaat acgacgtcgc ctgcaactgg
      661 aaattggtct ttcaaaacta ctccgagtgc tatcactgtc cgcttgtgca tccgcagctc
      721 gataagctgt cgccgtccga cagcggacgc aacgacctga ccgccggccc ggtgctcgga
      781 ggcttctcgg agcttcgcgt gcacggcgcc agcttgacga ccaccggcca aaccctgcgc
      841 cccccgctgg gcggcgtgga cggcgacgac ctcgaccgcg tgtactacta cacgatcttt
      901 ccgtcgatgc tgctgagcct gcatccggac tacgtgatgg tgcactacgt cgagcccacc
      961 ggaatcgcac gcacccgcgt cacctgttcg tggctgttcg atcc
//
LOCUS       DASUCJ010001064         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1340993, whole genome shotgun sequence.
ACCESSION   DASUCJ010001064 DASUCJ010000000
VERSION     DASUCJ010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340993"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU90_12585"
     CDS             <1..297
                     /locus_tag="VFU90_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480671.1"
                     /translation="FLAAGLHAATYLMMSGAVNAVGHTFGKQPYDNSATNMQSLALLS
                     GGEGLHNNHHAAPTSARFALHKGEIDPGWWVVRTLVALHLAQVRHEDVKLKSAA"
     gene            complement(369..>1291)
                     /locus_tag="VFU90_12590"
     CDS             complement(369..>1291)
                     /locus_tag="VFU90_12590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5480672.1"
                     /translation="FDGIPLVDQRDIVRELVDLGYTDLWSAESGGYDAFTPLVAAAQW
                     APELRFGTAIVPVYTRGAHTLASTVASMCQAAPGRFALGIGTSSDVIVERWNGMTFDK
                     PYQRVRDTIRFLRASLAGEKVDEEYETFRVRGFRLGIKVPEQPPILVAALRQGMLRLA
                     GREGDGAIINWLSADDVKSVVPHVGAGKEIVARIFVLPVDDAGMARAVGKRAIASYMT
                     VPVYRAFHEWLGRGDDFADLWRLWAEGDRKAAAESIPDHVVDQLLVWGSPQACKEHIA
                     RYIDNGVTTPAPALFCGPDQLRDVIRALAR"
BASE COUNT          231 a          440 c          424 g          196 t
ORIGIN      
        1 ttcctcgccg ccgggctcca cgccgcgacg tacctgatga tgtcgggcgc cgtcaacgcg
       61 gtggggcaca cgttcggcaa gcagccgtac gacaactcgg ccaccaacat gcagtcgctc
      121 gcattgctca gcggcggcga gggtctgcac aacaaccatc acgcggcgcc gacgtcggca
      181 cgttttgcgt tgcacaaagg cgagatcgac ccgggctggt gggtcgtgcg cacgctcgtc
      241 gcgctgcatc tcgcacaagt gcgccacgaa gacgtgaagc tgaagtccgc ggcgtgaaac
      301 ctcaccatca gttggaagag ttagctaccg gagcggtgca taactcttcc aacagatggg
      361 ggagttcgct accgggcgag ggcgcggatg acgtcgcgga gctggtcggg gccgcagaac
      421 aacgcgggtg ccggagtcgt gacaccgttg tcgatgtagc gggcgatgtg ctccttgcac
      481 gcttgcggcg agccccacac gagcaactgg tcgacgacgt ggtcgggaat cgactccgcc
      541 gccgccttgc ggtcgccttc ggcccacagc cgccacagat ccgcgaaatc gtcgccccgg
      601 ccgagccatt cgtggaacgc gcgatacacc ggcacggtca tgtacgacgc gatcgcgcgc
      661 ttgccgaccg cacgcgccat gccggcgtcg tcgaccggca gcacgaagat gcgcgcgacg
      721 atctccttgc cggccccgac gtgcggcacg acggacttca cgtcatcggc cgacagccag
      781 ttgatgatcg cgccgtcgcc ttcgcggccg gcgagccgca gcatgccctg ccgcagcgca
      841 gcaacgagga tcggcggctg ctcgggcact ttgatgccga gccggaatcc gcgtacacga
      901 aacgtctcgt actcctcgtc gaccttctcg cccgcgagcg acgcgcgcaa gaaccggatc
      961 gtgtcgcgca cacgttggta cggcttgtcg aacgtcatgc cgttccaacg ttcgacgatg
     1021 acgtcggagc tcgtcccgat ccccaatgcg aaccggccgg gcgccgcttg gcacatcgac
     1081 gcgaccgtcg acgcgagcgt gtgcgcgcct cgcgtgtaga cgggcacgat cgcggtaccg
     1141 aaacgcaact ccggagccca ttgcgcggcg gcgaccaacg gagtgaacgc gtcgtagccc
     1201 ccggactcgg ccgaccaaag gtcggtgtaa ccgagatcga cgagctcgcg cacgatgtcg
     1261 cgctggtcga cgagcggtat gccgtcgaac g
//
LOCUS       DASUCJ010001065         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_164047, whole genome shotgun sequence.
ACCESSION   DASUCJ010001065 DASUCJ010000000
VERSION     DASUCJ010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164047"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1037
                     /locus_tag="VFU90_12595"
     CDS             <1..>1037
                     /locus_tag="VFU90_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480673.1"
                     /translation="DRYSDYSSFGSTSNGKVALEWRPVEDLLLRGTVSEVFRAPNVTD
                     IYAGPQSSFPVLSHDPCDGYTGNPPNPACVHVPTDGTFRNLDVAQQLPINGVFAGPNY
                     AGLELGPEYGRSYDFGIVYDPNWLEGVSLSADAWRIYIKNNITRINAQSVLDLCAAGF
                     TTYCPLVRRVASGPDVGQIQSITETTINLGRTDVSGVDFALSYRLPEFSFGRFRITFN
                     ATYMAKFDAATAPGTDANQLYHYAGHYLPPGSTQATLCPGASGGVCLYPRWRALSSLN
                     WQHGPFDASWRLRYIGRFTLGSQSPSQDVFPAGTCYYGSYCTLHNYVIDYGATVYHDL
                     QFGYNLDAWNT"
BASE COUNT          179 a          378 c          305 g          175 t
ORIGIN      
        1 gcgatcgcta ctcggattat tcgagcttcg gctcgacgag caacggcaaa gtcgcactcg
       61 aatggcgacc cgtcgaggac ctgctgctgc gcggcacggt gtccgaagtc ttccgcgcgc
      121 cgaacgtcac cgacatctat gcagggcccc agagcagttt ccccgtgctg tcgcacgatc
      181 cctgcgacgg ctacaccggc aatccgccga acccggcgtg cgtgcacgtg ccgaccgacg
      241 ggacgttccg caacctcgac gtcgcgcagc agctgccgat caacggcgtc ttcgcgggcc
      301 cgaactacgc gggcctcgag ctcggccccg aatacggccg ctcgtacgac ttcggcatcg
      361 tctacgaccc gaactggctc gaaggtgttt cgctcagcgc cgatgcatgg cgcatctaca
      421 tcaagaacaa catcacgcgg atcaacgcgc agtcggtgct cgatctgtgc gccgcgggat
      481 tcacgacgta ttgcccgctc gtgcggcgcg tcgcgagcgg gcccgacgtc ggccagatcc
      541 agagcatcac cgagacgacg atcaacctcg gccgcaccga cgtcagcggc gtcgacttcg
      601 cgctgagcta ccgcttgccc gagttctcgt tcggccgctt ccgcatcacg ttcaacgcga
      661 cctacatggc gaagttcgac gccgcgaccg cgcctggtac cgatgcgaac cagctctatc
      721 actacgcagg ccactacctg ccgcccggtt cgacgcaggc gacgctctgt cccggcgcaa
      781 gcggcggcgt gtgcctgtat ccgcgctggc gcgcgctgag ctcgctgaac tggcagcacg
      841 gaccgttcga cgcgtcctgg cgcctgcgct acatcggccg cttcacgctc ggttcgcagt
      901 cgccgtcgca ggacgtcttt cccgccggca cctgctacta cggcagctac tgcacgctgc
      961 acaactacgt gatcgactac ggcgcgaccg tctatcacga cctgcaattc ggctacaacc
     1021 tcgacgcgtg gaacacg
//
LOCUS       DASUCJ010001066         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_41723, whole genome shotgun sequence.
ACCESSION   DASUCJ010001066 DASUCJ010000000
VERSION     DASUCJ010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41723"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFU90_12600"
     CDS             <1..678
                     /locus_tag="VFU90_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU5480674.1"
                     /translation="CSTRNAEEAELFIVEGDSAGGSAKRARNPAFQAILPIRGKILNV
                     ERSRIDKMLRNEEIQALISAVGTGIGEEFDLQKLRYHKIIVMTDADVDGSHIRTLLLT
                     FFYRQLPEIVRAGFVYIAQPPLFGVKLGKDDRVYLKDEASLAAFEGEHEGRKLEVSRF
                     KGLGEMDWHELGETTMDPATRTLLQVSVEDAAIADEIFSTLMGDDVDARRGFIQENAH
                     DVRFLDI"
     gene            691..>1283
                     /locus_tag="VFU90_12605"
     CDS             691..>1283
                     /locus_tag="VFU90_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5480675.1"
                     /translation="MPDEPRILGNIEPIEIQEEMERSFLDYAMSVITSRALPDARDGL
                     KPVQRRILYGMYAEGMRPDRPHRKCANAVGEVMGKYHPHGDQAIYDALARMAQDFSLR
                     YPLIDGHGNFGSPDPNDRPAAARYTEARLAQLAMELVGEIDEDTVDFGETYDGRNREP
                     IVLPARFPNLLVNGGGGIAVGMATNIPPHNLTEVINAT"
BASE COUNT          261 a          408 c          404 g          210 t
ORIGIN      
        1 tgctcgacgc gcaacgcaga ggaagcggag ctgttcatcg tcgaaggcga cagcgccggt
       61 gggagcgcaa agcgcgcgcg caaccccgcg tttcaagcga tcctgccgat ccgcggcaag
      121 atcctcaacg tcgaacgttc acgcatcgac aagatgctgc ggaacgagga gattcaggcg
      181 ctcatctcgg cggtcggtac gggtatcggc gaagagttcg atctccagaa gcttcgctac
      241 cacaagatca tcgtgatgac cgacgccgac gtcgacggat cgcacatccg cacgttgctg
      301 ttgacgttct tctaccggca gcttcccgag atcgtgcgcg ccggcttcgt gtacatcgca
      361 cagccgcccc tgttcggcgt gaagctcggc aaggacgatc gcgtgtacct gaaggacgaa
      421 gcgtcactcg ccgctttcga aggggagcac gagggccgca agctcgaagt cagccgcttc
      481 aagggtttgg gcgagatgga ctggcacgag ctcggcgaaa ccacgatgga tcccgcgacg
      541 cgaaccctgc tgcaagtgtc cgtcgaagac gccgcaatcg ccgacgagat cttctcgacg
      601 ttgatgggtg acgacgtcga cgcgcgacgc ggcttcattc aagagaacgc gcacgatgtg
      661 cgcttcctcg acatctgagc gaacgaacgc atgcctgacg aaccccggat tctcgggaac
      721 atcgaaccga tcgagatcca agaggagatg gaacgctcct tcttggacta cgcgatgtcg
      781 gtcatcactt cgcgcgcgtt gcccgacgca cgcgacggtt tgaagccggt gcaacggcgg
      841 attctctacg gcatgtacgc cgagggcatg cgtcccgacc gtccgcaccg caagtgcgcg
      901 aacgcggtcg gcgaggtgat gggcaagtac caccctcatg gcgaccaagc gatctacgac
      961 gcactggcgc gcatggcgca ggacttctcg ttgcgctacc cgctcatcga cgggcacggc
     1021 aacttcggtt cgcccgaccc caacgaccgg ccggcggccg cgcgctacac cgaggcgcgg
     1081 cttgcgcagc tcgcaatgga attggtcggt gagatcgacg aggacaccgt cgacttcggc
     1141 gagacgtacg acggccgcaa tcgcgagccg atcgtgttgc cggcacgctt tccgaacctg
     1201 ttggtcaacg ggggtggcgg catcgccgtc ggaatggcga cgaacattcc gccgcacaac
     1261 ctcaccgagg tcatcaacgc gac
//
LOCUS       DASUCJ010001067         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_775477, whole genome shotgun sequence.
ACCESSION   DASUCJ010001067 DASUCJ010000000
VERSION     DASUCJ010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFU90_12610"
     CDS             <1..430
                     /locus_tag="VFU90_12610"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5480676.1"
                     /translation="LGGSVFTFSLAAALKKGPVVLYFYPAAFTKGCTIEAHDFAAAID
                     TYRALGATVIGVSHDDIGTLNKFSVSECRSKFPVAADADQKIMKSYDAVLAIQPRYAD
                     RISYVIAPDGRIIYAYQSLNPEKHVTNTLEALRKWEATKP"
     gene            complement(478..2643)
                     /locus_tag="VFU90_12615"
     CDS             complement(478..2643)
                     /locus_tag="VFU90_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEU5480677.1"
                     /translation="MTRPMEGHHSLGERQVAGVISAPGVITYVCPMHPQIRWPAPGYC
                     PICGMALEPEMPSVEDERNPELEDFARRFWWTLPLSIVGVVLAMGGHRIVSVTPTAMS
                     WLELALSTPVVLWAGWPFFVRWLASIRDGNPNMWTLIGTGVAAAYLYSVLGTLIPGVF
                     PAEFREGGRVAVYFEAASVIVSLTLLGQMLELRARSRTSAAIRALLGLQPKTARRIGP
                     DGTEEDIPHASVHIGDLLRVRPGEKVPVDGTVTEGRSSVDESMLTGEAIPVEKAAGDR
                     VIGATINGTGSLIMRAEKIGAETVLAQIVQMVAQAQRSRAPMQRLADRVAHYFVLAVV
                     GTSIVTAIVWWLIGPEPRGAYAILAAVSVLIIACPCALGLATPMSVMVASGRGATSGV
                     LFRDAEALETLRRIDTLIVDKTGTLTLGKPMFTRAISVPPYSEDDVLRIAASIDQAGE
                     HPLGVAIVDAARRRALKLARPVSFESATGIGVRGKVDGRTIALGNTVLMRDVGANAAP
                     LAAEAERLRAEGASVIYLAVDGRLAGMLAVTDPIKDSTPAAIDALHRAGIRVVMATGD
                     GRTTAEAVAQRLGIDEIHGEVRPQDKADLVAHLQAQGRRVAMAGDGINDAPALARANV
                     GIAMGTGTDVAMSSAQVTLVKGDLRGIARARQLSEATVHNMYENLTFALIYNVLGVPI
                     AAGILYPAFGILLSPLVAAAAMSMSSVSVVGNALRLRKA"
     gene            complement(2650..>3063)
                     /locus_tag="VFU90_12620"
     CDS             complement(2650..>3063)
                     /locus_tag="VFU90_12620"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEU5480678.1"
                     /translation="AMVIVGSRAAIAAPAPSIDVYKSPACGCCGQWVRHMRGNGFSVV
                     VHDVGDLDAVRARAGVPRALASCHTAWVGGYVLEGHVPALDVRKLLAERPKAVGLTVP
                     GMPASAPGMDLPGAIPYDVLLFQADGATRAWHSYR"
BASE COUNT          540 a         1087 c          957 g          479 t
ORIGIN      
        1 gcttggcggc agcgtcttca cgttctcgct cgccgcagca ttgaagaagg ggccggtcgt
       61 cctgtacttc tatccggccg cgttcacgaa aggttgcacg atcgaggcgc acgatttcgc
      121 ggcggcgatc gatacctacc gtgcactcgg cgcgacggtg atcggcgttt cgcacgacga
      181 catcggcacc ttgaacaagt tctcggtcag cgaatgccgg agcaaatttc cggtcgccgc
      241 cgacgccgac cagaagatca tgaaatccta cgacgccgtg cttgcgatac aacccaggta
      301 cgcggaccgt atctcgtacg tcattgcgcc ggatgggcgc atcatctacg cctaccaatc
      361 gctcaatccc gagaagcacg tcacgaatac cctcgaagcg ctgcggaagt gggaagcgac
      421 aaagccgtag agcctacttt tccgcatgcc ccggcgccgg cgtaaccgca ccgaaggtca
      481 ggccttgcgc agccttaacg cgttacccac gaccgatacc gagctcatcg acatcgccgc
      541 cgccgcaacg agcggcgaca gcaggattcc gaacgccgga tagaggatcc cagccgcgat
      601 cgggacaccc agcacgttat agatcagcgc gaaggtcagg ttttcgtaca tgttgtgcac
      661 ggtggcttcc gagagctgtc gcgcgcgtgc gatgccgcgc aggtcgccct tgaccagcgt
      721 gacctgggca ctgctcatcg ccacgtcggt cccggtgccc atcgcaatgc cgacattcgc
      781 tcgcgcgagc gccggggcgt cgttgatgcc gtctccggcc atcgcgacac gccggccctg
      841 cgcctgcaga tgtgcaacca gatccgcctt gtcctgcggg cgcacctcgc cgtggatttc
      901 gtcgataccg agccgctgcg ccaccgcttc ggccgtcgtc cggccgtcac cggtcgccat
      961 gacgacgcga attccggctc gatgcagcgc atcgatcgcg gcgggcgtcg aatccttgat
     1021 cggatcggtc accgccagca tgccggccag gcggccgtcg acggcgaggt agatcacgct
     1081 cgccccctcc gcgcgcaatc gctcggcctc cgcggcgaga ggcgccgcat tggcaccgac
     1141 gtcgcgcatc aacaccgtgt tgccgagcgc gatcgtgcgc ccgtcgacct tcccgcgaac
     1201 accgatcccg gtggcggatt cgaacgatac aggcctggcg agcttcagcg cacgacgtcg
     1261 cgccgcgtcg acgatcgcga cgccgagcgg gtgctcgccg gcctggtcga tgctggcggc
     1321 aatgcgcagc acgtcgtctt cggagtacgg aggcacgctg atcgcgcggg taaacatcgg
     1381 tttgccgagc gtgagcgttc cggtcttgtc gacgatcagc gtatcgatcc tccgcagcgt
     1441 ctcgagcgcc tcggcatccc ggaacagcac gccgctggtc gctccccgac cactggccac
     1501 catcaccgac atcggcgtcg cgagaccgag cgcgcaggga cacgcgatga tcagcaccga
     1561 aaccgcggcg aggatcgcgt aggcgccgcg cggttcgggg ccgatcagcc accagacgat
     1621 ggccgtcacg atcgaagtgc cgacgaccgc aagaacgaaa taatgcgcga cgcgatcggc
     1681 cagacgctgc atcggcgcac gactgcgctg cgcctgcgcg accatctgga cgatctgtgc
     1741 gaggaccgtc tcggccccga tcttctccgc ccgcatgatc aggctcccag tgccgttgat
     1801 cgtcgcgccg atgacgcgat cccccgctgc cttctcgacc gggatggcct cgccggtcag
     1861 catcgattcg tcgacgctcg aacgaccttc ggttacggtg ccgtcgaccg gcaccttctc
     1921 tcccgggcgg acgcgcagga gatcgccgat atgcacgctc gcgtgcggaa tgtcctcctc
     1981 ggttccatcg ggtccgatgc gacgtgcggt cttcggctgc agcccgagga gcgcacgtat
     2041 cgcggccgag gtccgtgacc gtgcccgcaa ctccagcatc tgtccgagca gcgtcaacga
     2101 gacgatcacc gacgccgcct cgaaatagac cgcgacgcgc ccgccctcgc gaaactccgc
     2161 gggaaagact cccgggatga gcgtcccgag cacgctgtag aggtaagccg cggcaacacc
     2221 ggtcccgatc agcgtccaca tgttgggatt accgtcgcgg atcgacgcta gccaccggac
     2281 gaagaacggc cagcccgccc agagcacgac cggcgtcgat agcgcaagct cgagccacga
     2341 catcgctgtc ggggtgacgg acacgatccg gtgtccgccc atcgccagca cgacacccac
     2401 gatgctcagc ggaagcgtcc accagaaccg gcgcgcgaag tcttcgagct cgggattgcg
     2461 ctcgtcctcg accgacggca tctcgggctc gagcgccatg ccgcagatcg gacagtaccc
     2521 gggtgctggc caccggatct gcgggtgcat cgggcagacg taagtgatca cgcccggggc
     2581 ggagatcacg ccggcaactt gccgttcacc gagcgagtga tgtccttcca tcgggcgagt
     2641 cacaccttgc tatcgatagc tgtgccaggc gcgcgtcgcg ccgtcggcct ggaacagcag
     2701 cacgtcgtag gggatcgcac ccgggaggtc catccccggc gcgctcgccg gcatccccgg
     2761 aaccgtcagg cccaccgcct tcggccgctc ggcgaggagc ttgcggacgt ccaacgcagg
     2821 gacgtgccct tccagcacgt agccaccgac ccaggcggtg tggcacgacg cgagtgcacg
     2881 cggcacccct gccctcgcgc gaaccgcatc gagatcgccg acatcgtgta cgacgacact
     2941 gaatccgttg ccgcgcatgt gccgcaccca ctgaccgcag catccgcacg ccgggctctt
     3001 gtagacatcg atgctcggtg ccggggcggc aatcgccgcc ctgctgccga cgatgaccat
     3061 ggc
//
LOCUS       DASUCJ010001068         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_423840, whole genome shotgun sequence.
ACCESSION   DASUCJ010001068 DASUCJ010000000
VERSION     DASUCJ010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423840"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFU90_12625"
     CDS             complement(<1..230)
                     /locus_tag="VFU90_12625"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480679.1"
                     /translation="MSALSDDALRAKTPEFKDRLAGGESLDAMLPEVFAVVREAGKRT
                     INMRHFDVQIMGGQVLYEGRIAEMKTGEGKTLV"
     gene            complement(360..>1107)
                     /locus_tag="VFU90_12630"
     CDS             complement(360..>1107)
                     /locus_tag="VFU90_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480680.1"
                     /translation="ADVIPTEKYPSQVLLFLAGNTSPYQSIAGPKPMLKVPGGVALDA
                     SNRIYVTNVQGASVQQFVLPSPSPTPKGSPTPSPTPSPSPSPSGSPSPSPTPSPTPTP
                     INITPRITIAGPNTHVVTPTGVDVDSDGNIYISDEGQLGVKNSAAILVFAPPKTKGVL
                     NVAPLRVIEGNRGSNKTMLTTPTDVKVDSKAGLIYVADSNHVLVFDIKAKGNVAPVAV
                     YNSPGAVTGLGLVPYGADRAEPPAAAAAAD"
BASE COUNT          172 a          356 c          365 g          214 t
ORIGIN      
        1 accagcgtct ttccctcgcc ggtcttcatt tcggcgatgc gcccttcgta gagcacctgt
       61 ccgcccatga tctgcacgtc gaagtgacgc atgttgatcg tgcgcttccc ggcttcgcgc
      121 accaccgcga agacttccgg cagcatcgcg tcgagcgact cgccgcccgc caaacgatct
      181 ttgaactccg gcgttttggc gcgcagggcg tcgtcggaga gcgcgctcat ttgcggctcg
      241 agaacattga ccgcgttggc cgtgcggcgc agacgcgcga cctcgcgcta gtttccgtcg
      301 atcagcgctt tcaggaagga catgggggta ctacagcttc tccatacgag atttcggccc
      361 tagtccgcgg cagccgcggc ggccggcggc tcggctcggt cggcgccgta cggcaccagc
      421 ccgagcccgg tgaccgcgcc cggtgaattg tacaccgcca ccggcgcgac gttgcctttg
      481 gccttgatgt cgaagacgag cacgtggttc gaatcggcca cgtagatcaa tccggccttg
      541 gaatcgacct tcacgtcggt cggcgtcgtc aacatcgtct tattactacc cctattgccc
      601 tcaatcactc gcagcggcgc cacgttgagc acgcccttgg tcttgggcgg cgcgaacacc
      661 aggatggcgg ccgagttctt gacgccgagc tgtccttcgt cggagatata gatgttgccg
      721 tcgctgtcga cgtccacgcc ggtcggcgtc acgacgtggg tattcggccc ggcgatcgta
      781 atccgcggcg taatgttgat cggcgtcgga gtcggtgacg gcgtcggcga cgggctcggc
      841 gagccgctgg gggacggcga aggcgacgga gtcggtgagg gcgtgggcga tcccttcggc
      901 gtcggcgacg gcgaaggcag cacgaactgc tgcaccgacg cgccctgaac gttcgtcacg
      961 taaattcggt tgctcgcgtc caacgcgacg ccgccgggga ctttcagcat cggcttcggc
     1021 ccggcgatcg actggtacgg cgacgtgttg ccggcgagaa acagcagcac ttggctggga
     1081 tacttctcgg tcgggatgac gtcggcg
//
LOCUS       DASUCJ010001069         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_958665, whole genome shotgun sequence.
ACCESSION   DASUCJ010001069 DASUCJ010000000
VERSION     DASUCJ010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958665"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFU90_12635"
     CDS             complement(<1..341)
                     /locus_tag="VFU90_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480681.1"
                     /translation="MSETVRPPTPGPKAIVLAHGTVAEALVAAVDLITGRGDRFSPLS
                     NQNLGAAQIDALLRERIDALGARVIFTDLPAGSCNFAACRVLKDRPDLIVVTGVNLPA
                     LLQYATRDGVS"
     gene            complement(334..>1138)
                     /gene="hprK"
                     /locus_tag="VFU90_12640"
     CDS             complement(334..>1138)
                     /gene="hprK"
                     /locus_tag="VFU90_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEU5480682.1"
                     /translation="MQVFGETEITYLLSLPEDRRRLVLERFFSFDVPCAVVTKRLDVP
                     NEMREIAAGHGVAVLRSSLKTTEFYRRVKPALDEEFAPSTTLHASLADVYGVGLLFVG
                     RSGIGKSECVLDLVERGHRLVADDIVIATRRGNDVLIGRGHELQRHHMEIRGVGVIDI
                     PSVFGIRSVRQQKRIEVVVQLEHWDQADQVDRTGLDGATTTILEVPIPRVTIPLNPGK
                     NITVVAEVVAMNHLLRYSGINPAEAFNERLISRMRAASDVRQYLAEDDE"
BASE COUNT          190 a          395 c          367 g          186 t
ORIGIN      
        1 tcggacacgc cgtcgcgcgt cgcgtactgc agcagcgccg gcaaattgac gccggtcacg
       61 acgatcagat cgggccggtc cttgagcacc cggcaggcgg cgaagttgca gctgcccgcc
      121 ggcaaatcgg tgaagatcac gcgcgcgcct aacgcatcga tgcgctcccg cagcagcgcg
      181 tcgatctggg ccgcgcccag attctggttc gagagcggcg agaaccggtc gccgcgtccg
      241 gtgatcaggt cgaccgccgc caccagcgct tcggccaccg tgccgtgcgc gaggacgatc
      301 gccttgggac ccggcgtcgg cggcctcaca gtctcactca tcgtcttccg ccagatattg
      361 ccgcacgtcg gacgctgcgc gcatgcggct gatgaggcgt tcgttgaatg cttcggcggg
      421 attgatcccg ctgtagcgca gcagatggtt catcgcgacg acctccgcca ccacggtgat
      481 gttcttcccc gggttgagcg gaatcgtcac gcgcggaatc ggcacctcga gaatggtcgt
      541 cgtcgcgccg tcgaggccgg tgcgatccac ctgatcggcc tgatcccagt gctcgagctg
      601 caccaccacc tcgatgcgct tctgctggcg caccgagcga atgccaaaga ccgatggaat
      661 gtcgatcacg ccgacgccgc gaatctccat gtgatgccgc tgcagctcgt gcccgcgtcc
      721 gatgagcacg tcgttgccgc ggcgcgtggc gatcacgatg tcgtcggcca ccagacggtg
      781 tccgcgctcg acgagatcga gcacgcactc cgatttgccg atgccgcttc gcccaacgaa
      841 cagcaggccg acgccgtaca cgtcggccag cgacgcgtgc agcgtggtgc tcggcgcaaa
      901 ctcttcgtcg agcgccggct tgacgcgccg gtagaactcg gtggtcttga gactcgaccg
      961 cagcaccgcg acgccgtggc cggcggcgat ctcgcgcatc tcgttaggca catccagccg
     1021 cttggtgacc accgcgcacg ggacgtcgaa cgagaagaac cgctcgagca ccaggcggcg
     1081 gcggtcttcc ggcagcgaca gcaggtacgt gatctcggtc tcgccgaaca cctgcatc
//
LOCUS       DASUCJ010001070         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_286298, whole genome shotgun sequence.
ACCESSION   DASUCJ010001070 DASUCJ010000000
VERSION     DASUCJ010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286298"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1124
                     /locus_tag="VFU90_12645"
     CDS             23..>1124
                     /locus_tag="VFU90_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEU5480683.1"
                     /translation="MPSTWTEGEPLGAYLERRSVSRRDFIAFCTEMVAVLGLSTRSVQ
                     HVVRKLEMLKRPSVIWLQLQECTGCVESVIRTSDPTIGDLLLDVISLDYQHTLMAAAG
                     AQAELALKQSLAANAGHYVLVVTGSIPTKDGGIYCTIGGRTAHDVLEEAAQGAAAIVA
                     VGACAHWGSVQAAKPNPTGAVGVSDIIKDKPVLNIAGCPPIADVVTATLVHYLTFDRL
                     PETDGDGRPLFAYANRIHDTCPRRAHYDAGQFANQFDDEGARRGYCLYHVGCKGPETF
                     SPCPIFLWNSRTSFPIGAGHPCIGCTERNFWDTMTPFYHRLPNVGGIVVERTADLIGA
                     SLAIGATAGVVAHAVATGVHQLHERRKARELPV"
BASE COUNT          187 a          375 c          376 g          186 t
ORIGIN      
        1 cgggagacgc atgctcagtc cgatgccttc cacgtggacc gagggtgagc ccctcggcgc
       61 gtatctcgag cgccggtcgg tgagccgccg cgacttcatc gcgttttgca ccgagatggt
      121 cgccgtgctg ggtttgagca cgcgctccgt gcagcacgtc gtccgaaaac tggagatgct
      181 gaagcgtccc agtgtcatct ggctgcaatt gcaggagtgt accggctgcg tcgagagtgt
      241 gattcgcacg tcggatccga ccatcggcga cctgttgctg gacgtgatct cgctcgacta
      301 ccaacacacg ctgatggccg cggcgggcgc gcaagcggag ctcgcgctca agcagtcact
      361 cgcggcgaac gccgggcact acgtgctcgt tgtcaccggc tcgatcccga ccaaggacgg
      421 cgggatctac tgcacgatcg gcggtcgaac cgcgcacgac gtgttggagg aggcggcgca
      481 aggcgcggcg gcaatcgtcg ccgtcggcgc ctgcgcgcac tggggtagtg tgcaggcggc
      541 gaagccgaat cccaccggag ccgtcggcgt gtccgacatc atcaaggaca agccggtcct
      601 caacattgcc ggctgtccgc cgatcgccga cgtcgtgacg gcaacgctcg tgcactacct
      661 cacattcgac aggctgcccg agacggacgg cgacgggcgt ccgttgttcg cctacgcgaa
      721 ccgcatccac gacacgtgcc cgcgccgtgc gcactacgac gccggccagt ttgcgaatca
      781 gttcgacgac gaaggggcgc gacgcggcta ctgcctctat cacgtcggat gcaaagggcc
      841 ggagacgttc tcaccctgcc cgatctttct gtggaattcg cgtacgagct ttccgatcgg
      901 cgccgggcat ccgtgcatcg gatgcaccga gcgcaacttc tgggacacga tgacgccgtt
      961 ctatcaccgt ctgcctaacg tgggcgggat cgtcgtcgag cgcacggcgg acctcatcgg
     1021 ggcgtcgctc gccatcggcg cgacggccgg cgtcgtcgcc cacgcggtgg cgacgggcgt
     1081 gcaccagctg cacgagcgcc ggaaagcgcg cgagctgccc gtca
//
LOCUS       DASUCJ010001071         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1310285, whole genome shotgun sequence.
ACCESSION   DASUCJ010001071 DASUCJ010000000
VERSION     DASUCJ010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310285"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFU90_12650"
     CDS             complement(<1..449)
                     /locus_tag="VFU90_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480684.1"
                     /translation="MLTRTACVHAAAWEEMFDAFLRDRAARTNEPFAPFDPVADYGGY
                     IDGKLRYDGVRSFLTARGIELPEGAASDPPDANTVHGLGNRKNELLLSLIRTKGVDAY
                     DGSVRYLRAAENAGLRRAVVSASANARDVLDAAGIADLFEVRIDGIVA"
     gene            complement(622..>1937)
                     /locus_tag="VFU90_12655"
     CDS             complement(622..>1937)
                     /locus_tag="VFU90_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU5480685.1"
                     /translation="AFGSIASTLSIPLFIAAVGGGGRLMLHLYLAPLIFALIGLAFYT
                     VLGGADFGAGFWELTAGMGPQVQAVREHAHAAMAPVWEANHVWLIFVLTVVWTAYPTA
                     FGSIASTLSIPLFIAAVGIIFRGVTYALRAGAAGPRELSAIDTVFSISSVLTPFALGT
                     VVGGIASGRVPVGNAAGSQFSSWLNPTSLLVGVLAVTTAAYLAAVFLCADATKVGDRG
                     LEERFRRRALGAGIVAGAIAVVGLGILAGDAHRIFQRLTTGAGLAGIIVSLAAGVATL
                     ALVWRRKYELSRYSAAVAVAAIIAGWALAQSPLFLPGLTIKAAAAPRTTLIAVTVAVL
                     GGAVILFPSLALLFRLFLSGRLVEGGERVDLRPTPQLVRASRQGFLSRAALACLLAGI
                     GLLTAAETAWAHALGVVSLFGFVAFGFPAALPPRVIPYTSDAKRR"
BASE COUNT          363 a          636 c          687 g          251 t
ORIGIN      
        1 gcgacgatcc cgtcgattcg cacctcgaac agatccgcga tcccggccgc gtcgagaacg
       61 tcgcgcgcgt tcgcgctcgc ggagaccacg gcgcgccgga gtcccgcatt ctctgccgcc
      121 cgcaagtagc gtacggagcc gtcgtaggcg tcgactccct tcgtccggat cagcgagagc
      181 agaagttcgt tcttccggtt cccgagcccg tgaaccgtgt tcgcgtccgg cggatccgac
      241 gccgcacctt caggcagctc gatcccgcgc gcggtgagga acgagcgcac accgtcgtag
      301 cgcagcttgc cgtcgatgta gccgccgtag tcggcgaccg gatcgaacgg agcaaacggc
      361 tcgttcgtcc gcgcggcgcg atcgcggagg aacgcgtcga acatctcttc ccacgccgcc
      421 gcgtgcacgc aagcagtccg cgtcagcacc ccgtccaagt cgaaaagaca cgcccggatc
      481 gtgcgaggta gccctaaagg gtggtcgcaa gacacgacgc atacagtcat cggctccctc
      541 gggcactcgg agaggatcac ctgcgcaggc gtcggccgtc cgcccagtcc tcctcagagt
      601 gtccatgctg tcacgtcgct gtcagcgccg cttcgcgtcg ctggtgtatg gaatgacgcg
      661 cggcggcagg gcggccggga agccgaaggc aacgaagccg aagaggctga cgaccccgag
      721 cgcgtgcgcc caggcggttt ccgcagctgt tagtaacccg attccggcaa gaagacaggc
      781 gagcgcggcc cgtgaaagga aaccttgtcg cgacgctcgg accagttggg gcgtcgggcg
      841 gagatcgact ctttcgccac cttcgacgag tcggccgctg aggaagagcc gaaacaacag
      901 cgcgagggaa ggaaagagga tgaccgcgcc gccgagcacc gcgacggtca cagcgatcag
      961 ggttgtgcgc ggtgcggccg ctgctttgat cgtgagcccc ggcaggaaaa ggggcgactg
     1021 cgccagcgcc cagccggcga tgatcgcagc caccgccacc gcagcgctgt agcgggagag
     1081 ctcgtacttc cggcgccaga ccagtgccag cgtcgcgacc ccggcggcga gcgagacgat
     1141 gatcccggct agaccggctc cggttgtgag ccgttggaag atgcggtgag cgtcgccggc
     1201 caggatgccg aggccgacga ccgcaatggc gcccgcgaca atccctgcgc cgagtgcgcg
     1261 gcgacggaat cgctcctcga gcccacggtc gccgaccttg gtcgcgtccg cgcagagaaa
     1321 gaccgcagcg aggtaggcgg cagtcgtgac cgcgagcacg ccgaccagca gcgaggtcgg
     1381 gttgagccag ctgctgaact ggctgccggc ggcgttaccg acggggacgc ggccggaggc
     1441 gatgccgccg acgaccgtcc cgagtgcgaa aggcgtcagc actgaagaga tcgagaagac
     1501 ggtgtcgatc gcgctgagct cgcgtgggcc tgccgctccg gcgcggagcg cgtaggtaac
     1561 gccgcggaag ataatcccga ccgcggcgat gaagagcggg atcgagagcg tggaggcaat
     1621 cgatccgaag gcggtcggat acgcggtcca gacgacggtg agcacgaaga tgagccagac
     1681 gtggttggcc tcccacacgg gcgccatcgc tgcgtgcgcg tgctcgcgga ccgcctgcac
     1741 ctgcgggccc atgccggcgg tgagctccca gaagccggcg ccgaagtcgg cgccgccaag
     1801 cacggtgtag aaggccagcc cgatcagcgc gaagatcaac ggggccagat acaggtgcag
     1861 catcagtcga ccgccgccgc cgacggcggc gatgaagagc gggatcgaga gcgtggaggc
     1921 aatcgatccg aaggcgg
//
LOCUS       DASUCJ010001072         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1417491, whole genome shotgun sequence.
ACCESSION   DASUCJ010001072 DASUCJ010000000
VERSION     DASUCJ010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417491"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFU90_12660"
     CDS             <1..844
                     /locus_tag="VFU90_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU5480686.1"
                     /translation="YTALEHEPMHHETLLYIIHQLDSADKARVEQQHRDSPSVSPAMR
                     TVDAGIATLGAARDEIPFGWDNEFERTRVDVPAFEIDAYPVTNGDWLRFVAGGGPVPQ
                     FWFERDGAWYLRAQFDELPLPRSWPVYVTHRQARAYAAWAGKRLPTEAEYHRAAFAAP
                     GGQERAFPWGDDPPGPAFGNFDFQRFDPEPVDAHPAGASAWGVHDLIGNGWEWTATPF
                     EPLPGFQPMASYPQYSADFFDGRHYVMKGASPVTAAELIRRSFRNWFYDDYPYMYAKF
                     RCVA"
     gene            871..>1878
                     /locus_tag="VFU90_12665"
     CDS             871..>1878
                     /locus_tag="VFU90_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5480687.1"
                     /translation="MQRFLCVLTAVAALAAAGCSKGGQNAAPADELRLAININPMQLD
                     PILSQNTMESFVDGIIFDELVSQDAQHHQIPDLAATVPTLANGGIGKDGLTLTYHLRH
                     GVRWHDGAPFTAKDVLFTWHAIMNPANNVVSRRGYDQVQSMDAPDDYTVVMHMKRMFA
                     PAIDTIFGESDTPMRILPAHLLAKYPNINQVAFNAAPVGTGPYEFVRWLRGDRIVLRA
                     NPNYFRGAPRLKRLTLLIIQDDNTTEAQLRSHEVDLGMEIPAPVYRDVADAPGVVRQL
                     VEAPVYTAILFDMKHAPLDDVRIRRALVMGMDRRAIVRDDTYGTGKLAVADLSPYYWA
                     YD"
BASE COUNT          326 a          664 c          576 g          312 t
ORIGIN      
        1 ctacaccgcg ctcgagcacg aaccgatgca tcacgagacg ctgctctaca tcattcacca
       61 gctcgattcc gcggacaagg cgcgcgtcga gcagcagcac cgcgattcgc cgtcggtctc
      121 gccggcgatg cgcacggtag acgcgggaat cgccaccctc ggcgcggcgc gcgacgaaat
      181 tccgttcggc tgggacaacg agttcgaacg cacgcgcgtc gacgtgccgg ccttcgaaat
      241 agacgcgtat cccgtcacca acggcgactg gctgcgcttc gtcgccggcg gcggcccggt
      301 gccgcagttt tggttcgagc gcgacggcgc gtggtatctg cgcgcgcagt tcgacgagct
      361 gccgctgccg cgttcgtggc cggtgtacgt cacccaccgg caagcgcgcg cgtacgccgc
      421 ctgggcgggg aagcgcctgc ccaccgaagc cgagtaccat cgcgccgcct tcgccgcccc
      481 cggcggccag gagcgcgctt tcccatgggg cgacgatccg cccggcccgg ctttcggcaa
      541 cttcgatttc cagcgcttcg atcccgaacc ggtcgacgcg cacccggccg gcgccagcgc
      601 gtggggcgta cacgacttga tcggcaacgg ctgggaatgg acggcgaccc cgttcgaacc
      661 gcttcccggc ttccagccga tggcgtcgta cccgcagtac tccgccgatt tcttcgacgg
      721 ccggcactac gtcatgaagg gcgcttcgcc ggtaacggcg gccgagctga tccggcgcag
      781 ctttcgcaac tggttctacg acgactatcc gtacatgtac gccaagttcc gctgcgttgc
      841 gtagcaccgc gggacaccgg aggtaaggcg atgcagcggt ttttatgcgt gctgacggcc
      901 gtcgccgcgc tcgccgcagc gggctgctcc aaaggcgggc aaaacgccgc accggccgac
      961 gagctgcgcc tggcgatcaa catcaatccg atgcagctcg acccgatcct ttcgcaaaat
     1021 acgatggaaa gtttcgtcga cggcatcatc ttcgacgagc tggtctcgca ggacgcgcaa
     1081 caccatcaga tccccgacct cgccgcgacc gtcccgacgc tcgccaacgg cggcatcggc
     1141 aaagacgggc tcacgcttac ctaccatctt cgccacggcg tcaggtggca cgacggtgcc
     1201 ccgttcaccg ccaaagacgt gctcttcacc tggcatgcga tcatgaatcc ggccaacaac
     1261 gtcgtttcgc gtcgcggtta cgatcaagtc caatcgatgg atgctcccga cgattacacc
     1321 gtcgtcatgc atatgaagcg gatgttcgcg ccggcgatcg acacgatctt cggcgagagc
     1381 gacacgccga tgcggatcct cccggcgcac ctgctggcaa agtatcccaa catcaatcag
     1441 gttgcgttca atgcggcgcc ggtcggaacg gggccgtacg agttcgtccg ctggctgcgc
     1501 ggcgaccgga tcgtcctgcg ggccaatcca aactatttcc gcggcgcgcc gcgcctcaag
     1561 cggctgaccc tgctgatcat tcaagacgac aacaccaccg aggcccagct gcgctcgcac
     1621 gaagtcgatc tcggcatgga gattcccgcg ccggtttatc gcgacgtcgc cgacgcaccc
     1681 ggcgtcgtcc gtcagctggt cgaggcgccg gtttacaccg cgattctctt cgacatgaag
     1741 cacgcgccgc tcgacgacgt gcgtatccgg cgggcgctcg tgatggggat ggaccgccgc
     1801 gccatcgttc gcgacgacac ctacggcacc gggaaactcg ccgtggccga cctctcgccg
     1861 tactactggg cgtacgac
//
LOCUS       DASUCJ010001073         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_806368, whole genome shotgun sequence.
ACCESSION   DASUCJ010001073 DASUCJ010000000
VERSION     DASUCJ010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806368"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFU90_12670"
     CDS             complement(<1..298)
                     /locus_tag="VFU90_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5480688.1"
                     /translation="MQFFERVAELKNDRVPFALAIVVSRRSPVSSHVGDRAIVRADGG
                     MEGFVGGACSRDIVRRQALDAIRSGTPRLVHIRPGAPPETAAAGEEVFVNMSCSS"
     gene            complement(277..732)
                     /locus_tag="VFU90_12675"
     CDS             complement(277..732)
                     /locus_tag="VFU90_12675"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEU5480689.1"
                     /translation="MNLSWQGHEVVTAPKSRVWAFINDPASIAQCVPDVVESHVLDDR
                     HVEAVVKVGVGPVRGNFKLKIELDPQPGGNRMNMKISGGGFGSVVDLTAGADVKAEGD
                     SSTSLDWSGSATMRGPIASIAGRVIDAQAQRVITETFANVRARCSSSSG"
     gene            complement(729..>1596)
                     /locus_tag="VFU90_12680"
     CDS             complement(729..>1596)
                     /locus_tag="VFU90_12680"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5480690.1"
                     /translation="IVRAGTRGQGQGHETTWAQIVAEELGLDPAQLLVEEGDTDTAPY
                     GLGTYASRSTPVAGAALAMAARRVRERARKIAAHVLEAPEDDVEWKDYKFQLKGIPSK
                     SITMKEVAFAAYTNPGPNEPGLEATYYYDPPNLTFPYGAYLCVVDVDKATGATSVRRF
                     LAIDDCGTVINPMIVEGQIHGGLTEGFAIAFMQEIAYDENGNNLQPNFTEYLIPTSLE
                     TPKWETGSTCTPSPHHPIGAKGVGESPNVGSPAAFVNAVMDALAPLGITNIDMPLTRD
                     KVWRAIRDAGAA"
BASE COUNT          244 a          531 c          505 g          316 t
ORIGIN      
        1 cggagctgca gctcatgttc acgaagactt cttcgccggc ggcggccgtt tccggcggag
       61 cgccggggcg gatgtgcacc agccgcggcg taccgctgcg gatcgcgtcg agcgcttgcc
      121 gccgcacaat atcgcgggaa cacgctccgc cgacgaagcc ttccattccg ccgtcggctc
      181 ggacgatcgc gcgatcgccg acgtgcgagc tgaccggcga ccggcgcgaa acgacgattg
      241 cgagagcaaa cggtacgcgg tcgtttttga gttcggctac ccgctcgaag aactgcatcg
      301 cgccctgacg ttggcaaagg tttccgtgat gacgcgttgc gcttgcgcgt cgatcacgcg
      361 gccggcgatg ctcgcgatcg gcccgcgcat ggtagcgctc ccgctccaat cgagcgaagt
      421 cgacgaatcg ccctcggcct ttacgtcggc gcccgccgta agatcgacga cgcttccgaa
      481 accgccgccg ctgatcttca tgttcatccg gtttccgccg ggctgcgggt ccagctcgat
      541 cttgagcttg aagttaccgc gtaccggccc cacgccgacc ttcacgaccg cttccacgtg
      601 acggtcgtcc agaacgtgcg actcgacgac gtcggggacg cactgcgcga tactcgccgg
      661 atcgttgatg aacgcccaga cgcgcgattt cggcgccgtc acgacctcgt gaccctgcca
      721 gctgagattc acgcggcgcc cgcatcccgg atcgcacgcc acaccttatc gcgggtaagc
      781 ggcatatcga tgttggtaat cccgagcgga gcgagcgcgt ccatcaccgc gttgacgaag
      841 gcggccggcg agccgacgtt ggggctttcg ccgacgccct tcgcgccgat cggatggtgc
      901 gggctgggcg tgcacgtcga gccggtttcc cacttgggcg tttccagcga cgtcggaatc
      961 aagtattcgg tgaagttcgg ctgaaggttg ttgccgttct cgtcgtaggc gatttcctgc
     1021 atgaacgcga tcgcgaaacc ttccgtcaaa ccgccgtgta tctgtccctc gacgatcatc
     1081 ggattgatga cggtcccgca gtcgtcgatc gcaaggaagc ggcgaacgct ggtcgcgccg
     1141 gtcgccttgt cgacgtcgac gacgcacaga tacgctccgt acgggaacgt cagattcggg
     1201 ggatcgtagt agtaggtcgc ctccagtccg ggttcgttcg gcccggggtt cgtgtacgcg
     1261 gcgaaggcga cttctttcat cgtgatcgac ttcgacggaa tccctttgag ctggaacttg
     1321 taatctttcc attccacgtc gtcttcgggc gcttccagca cgtgggccgc gatcttccgg
     1381 gctctttcgc gcacccggcg tgcggccatc gcgagcgccg cgccggcaac cggcgtactg
     1441 cggctggcgt acgtgccgag gccgtacggc gcggtgtcgg tgtcgccttc ctctaccagc
     1501 agctgtgcgg ggtcgagtcc cagctcttcc gctacgattt gcgcccaggt cgtttcgtga
     1561 ccctgaccct gccctctcgt tccggcgcgc acgatt
//
LOCUS       DASUCJ010001074         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_943759, whole genome shotgun sequence.
ACCESSION   DASUCJ010001074 DASUCJ010000000
VERSION     DASUCJ010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943759"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1166)
                     /locus_tag="VFU90_12685"
     CDS             complement(<1..>1166)
                     /locus_tag="VFU90_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU5480691.1"
                     /translation="AELYIEREEKVIGSIFKGKVQNVLPGMGAAFVDIGLGRNAFLYV
                     DDINKQPLNIGDVEITQGHSGFTIGEKVKRGDDILVQIVKEPRGLKGARISTNISLPG
                     RYLILMPTGKYSGVSRKIESAQERNRLKAVMKRIRPEGMATVVRTAAGGVSEAELIAD
                     LGVLIRMWHGILEMYKRASAPSLLHKDMNLIYKAARDFVTADVDRVLIDDEDEYRKVR
                     DFLQLLGPQDLDRVEYYQGGRALFDDFRINEELQTLMKPKINLPSGGSIVIESTEALT
                     VIDVNSGKFTGGRNLEDTILKTNIEAAHEIARQVRLRDIGGIIVVDFIDMSSEASRSK
                     VIKTLEDGLRRDRTRATIQSFSNLGLLEFTRKRVGKDLGAQLRGACPTCAGMGS"
BASE COUNT          192 a          363 c          360 g          251 t
ORIGIN      
        1 cgctgcccat gccggcgcac gtcgggcacg cgccgcgaag ctgcgcgccc aagtctttgc
       61 cgactcgctt gcgggtgaac tcgagcagcc ccaagttcga aaacgattga atcgtcgcgc
      121 gcgtgcgatc gcggcgcaaa ccgtcctcga gggttttgat gaccttgctg cgcgacgcct
      181 cggacgacat gtcgatgaag tcgacgacga tgatgccgcc gatatcccgg aggcgcacct
      241 gtcgcgcgat ttcgtgggcg gcctcgatat tggtcttgag gatcgtgtct tcgagattcc
      301 gcccgccggt aaactttccg gagttcacgt cgatgacggt gagcgcttcg gtggattcga
      361 tcacgatcga tccgcccgac ggcaagttga tcttcggttt catgagcgtc tgcagctctt
      421 cgttgatgcg gaaatcgtcg aacagcgcgc ggccgccctg gtagtactcg acgcggtcga
      481 gatcttgcgg cccgagcagc tgcaggaaat cgcgcacctt gcgatactcg tcctcgtcgt
      541 cgatgagcac ccggtccacg tcggcggtca cgaaatcgcg cgccgctttg tagatgaggt
      601 tcatgtcctt gtgcagcagc gacggcgccg aggctcgctt gtacatttcc agaattccgt
      661 gccacatacg gatgagcacg ccgagatccg cgatcagctc ggcttcgctg acgccgccgg
      721 cggccgtgcg cacgaccgtc gccatgcctt ccggccgtat ccgcttcatc accgccttga
      781 gacggttgcg ctcctgcgcc gattcgatct ttcgggacac gcccgaatac ttgccggtcg
      841 gcatgaggat caggtagcgc ccgggcagcg aaatgttcgt cgaaatgcgc gcgcccttga
      901 gcccgcgcgg ttccttgacg atctggacga ggatgtcgtc gccgcgcttg accttctcgc
      961 cgatggtgaa gccgctatgg ccctgcgtga tttcgacgtc gccgatgttc aacggctgtt
     1021 tgttgatgtc gtcgacgtag agaaacgcgt tgcggccgag gccgatgtcg acgaacgccg
     1081 cccccatgcc cgggagcacg ttttgaactt ttcctttgaa gatcgagccg atgacttttt
     1141 cttcgcgctc gatgtagagc tcggcg
//
LOCUS       DASUCJ010001075         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_255620, whole genome shotgun sequence.
ACCESSION   DASUCJ010001075 DASUCJ010000000
VERSION     DASUCJ010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255620"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1076
                     /locus_tag="VFU90_12690"
     CDS             <1..>1076
                     /locus_tag="VFU90_12690"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5480692.1"
                     /translation="GTSVRVSFARPGVTAPIGLTFTRAVIHIPAVPFAMMFDGKVGYV
                     PLQQFNETSADEFENAVRRLTHDGAHGLVIDLRGNGGGIVDQAIAISNLFLRNGQQIA
                     SVRARGGDTQVYLAKGDPVAPTIPLVVLTDGYTASASEIVAGALQDHDRALVLGTTSF
                     GKGLVQSLFPLDGGWALKLTTAKWYTPSGRSIQKERKFIDGRLIDEPDSVETDSARRA
                     RPTFKSDAGRVVYGGGGITPDIVVRPDTLDSTEQEFVKAVTPKSQEFYVTLYNYAFEL
                     KNHVQPDFTVSQQWRDELYKRLAAAGVTVDRGLYDRASSEVDRLLSDRVARLAFGDST
                     AKRRELDDDVQLERAIEIIRKGQT"
BASE COUNT          182 a          342 c          376 g          176 t
ORIGIN      
        1 cgggacctcg gtgcgcgtct cgttcgcgcg ccccggcgtc acggcgccga tcggcctcac
       61 gttcacgcgc gccgtgatcc acattccggc cgtgccgttc gcgatgatgt tcgacggcaa
      121 ggtcggctac gttccgctgc agcagttcaa cgagacgtcg gccgacgagt tcgagaacgc
      181 ggtccgtcgc ctaacgcacg acggcgcgca cgggctggtg atcgatctcc gcggcaacgg
      241 cggcggcatc gtcgaccagg cgatcgcgat cagcaacctg ttcctgcgca acggccagca
      301 gatcgcgagc gtgcgggcgc gcggcggcga cacgcaggtg tacctggcga agggcgatcc
      361 ggtggcgccg acgattccgc tcgtcgtgct cacggacggc tacacggcgt cggccagcga
      421 gatcgtggcc ggcgcgctgc aggatcacga tcgcgcgctc gtgttaggca cgacgtcgtt
      481 cggcaagggg ctggtccagt cgttgttccc cctggacggg ggctgggcgc tcaagctcac
      541 gaccgccaag tggtacacgc cgagcggtcg gtccattcag aaggagcgca aattcatcga
      601 cggccggttg atcgatgagc ccgattcggt ggagaccgac tcggcgcggc gtgcgcggcc
      661 gacgttcaag agcgatgccg gccgcgtggt gtatggcggc ggcgggatca caccggacat
      721 cgtggtgcgg cctgatacgc tcgacagcac cgagcaggag ttcgtgaagg cggtgacacc
      781 gaagtcgcag gagttttatg tcacgctcta caactacgcg ttcgagctca agaatcacgt
      841 ccagcccgac ttcaccgtta gtcagcagtg gcgcgacgag ttgtacaagc gcctcgcggc
      901 ggccggcgtc acggtggatc gcggcctgta cgatcgggcc tcgagcgagg tggaccgcct
      961 gctcagcgac cgtgtcgcgc gtctggcgtt cggcgattcg acggccaagc gtcgcgagct
     1021 cgatgacgat gtgcagctcg agcgtgccat cgagatcatt cgaaaaggac aaaccc
//
LOCUS       DASUCJ010001076         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_118567, whole genome shotgun sequence.
ACCESSION   DASUCJ010001076 DASUCJ010000000
VERSION     DASUCJ010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118567"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFU90_12695"
     CDS             <1..827
                     /locus_tag="VFU90_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5480693.1"
                     /translation="PRNQLDSDKAKLDQAQVAYDQSVQQARLGAISGYNGSSVQYAAA
                     TARKAQIVNAQNQQQLGFTRISAPFDGIVQTVASQASDPMRSLQPGDAVTEGEALFTI
                     AGGGGYIVKAQIDEQDVINVRLGQRVNVSGQDFPGKTIPGVVARIAPVATKSTDASST
                     AKQVLATIALDSSPAYLRDGMTADVDILTTYVPHAVVVPNDAVIKEGSKSYVFVVTAG
                     KAHKRPIVVGKAAETQTWVKSGLAPGETIVAAKAPGLTDGTRVTPMPSPSALPSGP"
     gene            824..>1516
                     /locus_tag="VFU90_12700"
     CDS             824..>1516
                     /locus_tag="VFU90_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480694.1"
                     /translation="MTYLFSYFEEAAAALWRNRVRSILTMLGMIIGSASIIAVFGISR
                     AATSGITATFASFGQLPVFVAPDPSQDDPARAALHYRDAAAVAEALGDQAAAVYPNWQ
                     RTYQVSYRNVRDYESVVIDGGYHTDTLKMADGRKVDQNDVASAARVCVMTSDLAQKYF
                     GTAPAVGNFLHVNGTRCQVVGVYADIKGSFMNALAGSGLVLPYTTFNAVFAPGDLDSI
                     LVYPADQKQADVV"
BASE COUNT          285 a          511 c          472 g          248 t
ORIGIN      
        1 ttccgcgcaa ccagctcgac tccgacaagg cgaagctcga tcaggcgcag gttgcctacg
       61 accaatcggt tcagcaggcc aggctaggcg cgatttcggg atacaacgga agcagcgtgc
      121 agtacgcggc ggcgaccgcg cgcaaagccc aaatcgtcaa cgctcaaaat caacagcagc
      181 tcggctttac gcgcatctcg gcgccgttcg acggaatcgt tcaaaccgtc gcttcgcagg
      241 cgagcgatcc gatgcgctcg ctgcagccgg gcgacgccgt gaccgagggc gaagctttgt
      301 tcacgattgc cggcggcggc ggctacatcg tcaaggcaca aatcgacgaa caggacgtca
      361 tcaacgtcag gctgggtcag cgcgtaaacg tcagcgggca agatttcccc ggcaagacga
      421 tcccgggcgt cgtcgcccgg atcgcgccgg tcgccaccaa atccaccgac gcgagcagca
      481 ccgccaagca agtgctcgcg acgatcgcgc tcgattcgtc acccgcatac ttgcgcgatg
      541 ggatgaccgc cgacgtcgac atcctcacca cctacgttcc gcacgcggtc gtggtcccga
      601 acgacgccgt catcaaggaa ggctccaagt cgtacgtttt cgtggtgacg gcgggcaagg
      661 cgcacaaacg tccgatcgtc gtggggaagg cggccgagac gcaaacctgg gtgaaatccg
      721 ggctcgcgcc gggtgaaacg atcgtcgcgg ccaaagcgcc ggggctgacc gacgggacgc
      781 gcgtcacgcc gatgccgtcg ccgtcggcgt tgccgtcggg cccgtgacgt atctcttttc
      841 gtacttcgaa gaggccgctg ccgcgctctg gcgcaatcgc gttcgctcga ttttgacgat
      901 gctcgggatg atcatcggca gcgcttcgat tatcgccgtt ttcggaatca gccgcgcggc
      961 caccagcggg attaccgcga ccttcgcctc gttcggccag ctgccggtct tcgtcgcacc
     1021 cgatccgtcg caagacgatc cggcgcgcgc cgccctgcac taccgcgacg ccgccgcggt
     1081 ggccgaggcg ctcggggatc aagccgccgc agtgtatccc aactggcagc gcacgtatca
     1141 ggtttcctac cggaacgtgc gcgactacga gagcgtcgtc atcgacggcg gctaccacac
     1201 cgacacgctc aagatggcgg acggccgcaa agtcgatcaa aacgacgtcg cctcggcggc
     1261 gcgcgtctgc gtgatgacgt ccgatctggc gcaaaaatac ttcgggaccg cgcccgccgt
     1321 cggaaacttc ctgcacgtca acggcacgcg ctgccaagtc gtcggcgtct atgccgacat
     1381 caagggctcg ttcatgaacg cgctggccgg cagcggcctc gtgctgccgt acacgacctt
     1441 caacgcggtc tttgcgccgg gcgacctgga ttcgattctg gtctatccgg ccgatcagaa
     1501 gcaagccgac gtggtc
//
LOCUS       DASUCJ010001077         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1387133, whole genome shotgun sequence.
ACCESSION   DASUCJ010001077 DASUCJ010000000
VERSION     DASUCJ010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387133"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1430)
                     /locus_tag="VFU90_12705"
     CDS             complement(132..1430)
                     /locus_tag="VFU90_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEU5480695.1"
                     /translation="MTATIPETPAPRFDTVALHGGFGGDPATHARAVPIYQTTSYLFD
                     STEHAGKLFALEEFGNIYTRINNPTTDVLERRLAALEAGVGALAVASGQAAVVYSILN
                     LAAAGDRVVSSASLYGGTYNAFAHTLPRFGIQVDFVDFADLNALDAAITPGTKAVFAE
                     TIGNPKIDVLDVERVARIAHAHGVPLIVDNTLATPYLLRPIEWGADVVVHSATKFIGG
                     HGTAIGGAIVDGGTFDWAADPRFAEFVEPDPSYHGLSYTAAFGDRAYILKARVQLLRD
                     LGSALSPFNAWLLLQGLETLGVRIERHVANARAVAEFLDAHPRVASVSYPGLPGDPAH
                     ERARRYLPAGAGSILTFRIRGDGGDARAFIDRLKLFSLLANVGDAKSLVIHPASTTHQ
                     QLSPQERLRSGVDDSTIRLSIGIEDRRDILADLAQAFESA"
     gene            complement(1427..>2383)
                     /locus_tag="VFU90_12710"
     CDS             complement(1427..>2383)
                     /locus_tag="VFU90_12710"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5480696.1"
                     /translation="DDAEVDLVVECIGGTGDAAELVERALDRGRHVVTANKDLLATQG
                     PRLQALAAARGVTLRFEAAVAAAIPVVRTLDDALAGDPVAAIAGVVNGTCTSILSAME
                     RGRDYDAALSDARRDGFAEADPSADVDGTDAAHKLALLVQLAFRLAVISPRIRRTGIA
                     AIARRDVARAQMLGLRIRLVAAAIRSTHGTFAEVAPVLVRDDHEFGRTAGAENVVRVN
                     ARDAGSLTLRGAGAGGAATASAVLGDVATVLRAIGERHDFAQRTRASALDPAIDVSPL
                     FESFPRTSELPQYPLWDDTSFLDAPASQAALALSWPGKERTS"
BASE COUNT          382 a          778 c          868 g          355 t
ORIGIN      
        1 gttcgaacca tcggacgccg gttcgccgta gatcgtcacg cgctgatcga cggcagggag
       61 cgtttccccg ccgtcgagcg tcagcggtcc gatcgcgacg gtacgttcca gcacgtcgct
      121 cccggttgcg gtcacgcgct ctcgaatgcc tgcgcgagat cggcgaggat gtcgcgccgg
      181 tcttcgatgc cgatcgacag gcggatcgtc gagtcgtcca caccgctgcg cagccgctct
      241 tgcgggctga gctgctgatg ggtcgtcgag gccggatgga tgaccagcga cttcgcgtcg
      301 ccgacgttgg ccagcagcga gaagagcttc agacggtcga tgaacgcgcg agcgtcgccg
      361 ccgtcgccgc ggatgcggaa ggtcaggatc gatcccgcac cggcggggag gtaccggcgc
      421 gcccgctcgt gcgcgggatc gccgggaagg ccgggatacg aaaccgatgc gacccgcgga
      481 tgcgcatcga gaaactccgc gacggctcga gcgttggcaa cgtgccgttc gatccgcacg
      541 cccagcgttt ccaacccttg cagcagcagc cacgcgttga agggcgaaag cgccgacccc
      601 agatcgcgca gcagctgcac ccgcgctttg aggatgtagg cacgatcgcc gaaagcggcc
      661 gtgtaggaaa gcccgtggta ggacgggtcg ggctcgacga actcggcaaa gcgcggatcg
      721 gccgcccaat cgaacgttcc gccgtcgacg atcgccccgc cgatcgcggt gccgtggccg
      781 ccgatgaatt tcgtggccga atgcacgacg acgtcggctc cccactcgat cggacgcagc
      841 agatacgggg tggcgagcgt gttgtcgacg atcagcggga cgccgtgggc gtgcgcgatc
      901 cgcgccaccc gttcgacgtc gagcacgtcg attttcgggt tgccgatggt ttcggcgaag
      961 accgccttgg tgccgggcgt aatcgccgcg tcgagtgcgt tgaggtcggc gaagtcgacg
     1021 aagtcgacct ggatgccgaa gcggggtaac gtgtgcgcga acgcgttgta cgttccgcca
     1081 tacagcgacg ccgagctgac gacccggtcg ccggccgcgg cgaggttcaa gatcgagtag
     1141 acgacggcgg cctgaccgct cgcgaccgcg agcgcgccga cgccggcttc gagggccgct
     1201 aagcgccgct ccagcacgtc ggtcgtgggg ttgttgatgc gcgtgtagat gttgccgaac
     1261 tcctcgaggg cgaagagttt gccggcgtgc tcggttgagt cgaagagata cgacgtggtc
     1321 tggtagatcg gcacggcccg agcgtgggtg gcgggatcgc cgccgaagcc gccgtgcaat
     1381 gcgacggtgt cgaatcgcgg cgcgggagtt tccggaatgg tggcggtcac gaggtacgtt
     1441 cctttcccgg ccatgagagc gcaagcgcgg cttgcgaggc cggagcgtcg aggaaggagg
     1501 tgtcgtccca cagcggatac tgcgggagtt cgctggtgcg gggaaacgac tcgaacagcg
     1561 gcgatacgtc gatcgcggga tcgagcgcgc tcgcacgagt gcgctgggcg aagtcgtggc
     1621 gttcgccgat cgcgcgcagc acggtcgcga cgtcgccgag cacggccgaa gcggtcgccg
     1681 caccgccggc tccggcgccg cgcagggtga gcgatccggc gtcgcgtgcg ttcacgcgaa
     1741 cgacgttttc ggcgccggcc gtgcgtccga attcgtggtc gtcgcggacc agcacgggag
     1801 caacttcggc aaacgtgccg tgcgtcgaac ggatggcggc cgcaaccagc cggatccgca
     1861 gcccgagcat ctgcgcgcgc gcgacgtcgc gccgcgcgat cgcagcgata ccggtgcgcc
     1921 ggatgcgcgg cgagatgacc gccagccgga aagccagctg aaccagcagc gcgagcttgt
     1981 gcgccgcgtc ggttccatcg acgtcggcgc tgggatcggc ctcggcgaaa ccgtcgcgcc
     2041 gggcgtcgct cagcgcggcg tcgtagtcgc gtccccgttc catcgccgag aggatcgacg
     2101 tgcacgtccc gttgacgacg ccggcgatcg cggccaccgg atcgccggcc agcgcgtcgt
     2161 cgagcgtgcg gacgaccgga atcgccgccg caacggcagc ctcgaaacgc agcgtgacgc
     2221 cgcgagccgc cgccagcgcc tgcaggcgcg gaccttgcgt ggccagcaga tctttgttgg
     2281 cggtcacgac gtgacggccg cggtcgagcg cgcgctcgac gagttcggcc gcatcgccgg
     2341 ttccaccgat acactcgacg accaagtcga cctccgcgtc gtc
//
LOCUS       DASUCJ010001078         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1325791, whole genome shotgun sequence.
ACCESSION   DASUCJ010001078 DASUCJ010000000
VERSION     DASUCJ010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325791"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..>1309)
                     /locus_tag="VFU90_12715"
     CDS             complement(50..>1309)
                     /locus_tag="VFU90_12715"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5480697.1"
                     /translation="PLYLNPGPIERNGRVFVPLRGIFERLGSTVVYQSGTINATKGQT
                     TVTLHIGSTQALVNNQQQILDVAPFIVGATTYVPLRFVAQSLGAQVNYNGDTRLVAII
                     PVGGGGGGGGYYPPAPRPPYPPPYPPGNPAQLRAQQPYPGATTENRFATISAEFTRQV
                     RANTVRVWLDGNGITSRCNISSIGFSYRPPAPLEFGSHSVRVEGADVNGLTFNRSWSF
                     SVSGAPGPELRNQQPAPDSKTPDRFTVISADFSRPVDTGSVRILLDGNNITSRGGLSS
                     TGFSYKPPAPLDFGDHKVRVTGRGQGGNAFDRSWTFVVIRTMPTAPNMPLTINQPAPN
                     SSVGRTFLIQGNTGGNAQVTVTAGATPSSTGQFSGSTTAGPQGNFKINVTLTTLMGQS
                     SVTVKITATDPVSNQVKQTVLQLRLNQ"
BASE COUNT          211 a          393 c          457 g          248 t
ORIGIN      
        1 cctgagcgga gcgccgcagg cgcgcagtcg aagggcgctg tttaccccct tattgattca
       61 gccgtaactg cagcacggtt tgctttacct ggttgctgac cggatcggtg gcggtgatct
      121 tcaccgtgac gctgctttgg cccatcagcg tggttagcgt cacgttgatc ttgaagttgc
      181 cttgcggccc ggcggtggtc gatccgctga actgtccggt gctcgagggt gttgcgccgg
      241 ccgtaaccgt cacttgagcg ttgccgccgg tgttgccttg gatgaggaac gtccgtccga
      301 cgctcgagtt tggcgccggc tgattgatcg tcagcggcat gttcggcgcg gtcggcatcg
      361 tgcgaatcac gacgaacgtc caggaacggt cgaacgcgtt gccgccttga ccgcggccgg
      421 tcacgcgcac cttgtggtcg ccgaagtcga gcggcgccgg cggcttgtac gagaagcccg
      481 tcgacgaaag cccgccgcgc gacgtaatgt tgttgccgtc gagcagaatg cgcacgctgc
      541 cggtgtcgac cggccgtgag aagtctgccg agatcaccgt aaagcgatcg ggcgtcttcg
      601 aatcgggtgc gggctgctga ttgcgcagct ctgggccggg cgcgccgctc acgctgaacg
      661 accacgaacg gttgaaggtc aatccgttga cgtcggctcc ttcgacgcgc acggagtgcg
      721 atccgaactc tagcggcgcg ggcggccggt acgaaaaccc gatcgacgag atgttgcagc
      781 gcgacgtgat gccgttgccg tcgagccaaa cgcgcaccgt gttcgcgcgg acttggcgcg
      841 tgaactcggc cgagatcgtc gcgaagcgat tttcggtagt cgctccggga tagggctgct
      901 gcgcgcgcaa ctgtgccgga ttgcccggcg gatagggcgg cgggtacggc ggacgcggag
      961 cgggcggata gtaaccgccg cctcctcctc cgccgccgac cggaataatc gcgacgaggc
     1021 gcgtgtcgcc gttgtagttc acttgcgcgc cgagcgactg cgcgacgaag cgcagcggca
     1081 cgtaggtcgt agcaccgacg atgaacggag cgacatcgag gatttgctgc tgattgttga
     1141 ccagcgcctg cgtcgagcca atgtgtagcg taacggtcgt ctgccctttg gtggcgttga
     1201 tcgtcccgct ttggtacacg accgtcgaac ctaagcgctc gaaaatgccg cgcagcggga
     1261 cgaagacgcg cccgttgcgc tcgatcggcc cggggttgag gtacagcgg
//
LOCUS       DASUCJ010001079         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_179507, whole genome shotgun sequence.
ACCESSION   DASUCJ010001079 DASUCJ010000000
VERSION     DASUCJ010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179507"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFU90_12720"
     CDS             complement(<1..391)
                     /locus_tag="VFU90_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU5480698.1"
                     /translation="MGSQPHERVMRLADLGDVPLHLALVEPEIPPNTGNVARLCAATG
                     CALHLVEPLGFRIDDRALKRAGLDYWDNVRLLVHPSLDAFVAATEAMPRWFFSTRGAR
                     RYDHATFSAGSVLIFGKETKGLPGDVLA"
     gene            complement(397..1293)
                     /locus_tag="VFU90_12725"
     CDS             complement(397..1293)
                     /locus_tag="VFU90_12725"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU5480699.1"
                     /translation="MERLDESPAGFVQVRDAHESARLLFEPARGGVLSWNTFAPAGRI
                     EFRLLRARRPATDWIDYAEWHPNGSKSFSPDHEGVHVDVDVIRAEQPFDGIEARAAGI
                     DFNLVAFSSPLRPRPSLPYARDALILDVPVRSQYAVDGERGWCSPASLAMIHAYHGID
                     VPVETTARAVFDRAYNGTGNWTFNVAYSGSLGLCGVVAHLRNLDHAQRLIEQNLPLAI
                     SYSWREGELPGAPLERSDGHLAVLCGFTKSGDCAVNDPAAPNVRVVYPRAAVESLWQR
                     NEGVTYVVAPHGIDYARVLGSP"
     gene            complement(1281..>2168)
                     /locus_tag="VFU90_12730"
     CDS             complement(1281..>2168)
                     /locus_tag="VFU90_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5480700.1"
                     /translation="DVNTLFSELETHEIDVYPNVDANAVARLGGIAGIAVKKQLTANW
                     RHLGINMSRPLLRDIAVRRAIAEGVDWKRIDDTVYHGLNRLAVSDVFPESWAAPALPP
                     YRYDPAGARALLARAGWIRGGDGALHKGGSVLQLTISATIGHQENEQSEVLIQSMLRA
                     IGIDVRVRNYPANEMFAQNGPLYTGKYDLEWSIDTNGPDPDNTGNWNGAFIPPNGANT
                     SWLDDPIVNATSAAAAHTFDRRARKALYQREEERIRELVPAVFFSWEESYTAMNADVK
                     NYVPAAFIGDTWNSWQWSV"
BASE COUNT          346 a          693 c          758 g          371 t
ORIGIN      
        1 gcgccaggac gtcgccgggc aaacctttcg tttccttgcc gaatatcagc acgctcccgg
       61 ctgaaaacgt cgcatggtcg tagcggcgcg caccgcgcgt tgagaaaaac cagcgcggca
      121 tcgcctcggt ggcggcgacg aacgcgtcga gcgaaggatg gacgagcagc cggacgttgt
      181 cccaatagtc caagccggcg cgtttgagcg cccggtcgtc gatccgaaaa ccgagcggtt
      241 cgacgagatg gagcgcgcag ccggtagccg cgcagagccg cgcgacgttt ccggtgttcg
      301 gcgggatctc cggttcgact agcgcgaggt gcagcggaac gtcgcccaag tccgcgagcc
      361 gcatcacccg ctcgtgcggt tggcttccca cggcgtctac ggcgaaccga gcacgcgggc
      421 gtagtcgatg ccgtggggcg cgacgacgta ggtaacgcct tcgttgcgct gccaaagcga
      481 ctcgacggcg gcgcgcgggt agacgacgcg aacgttcggt gcggccggat cgttgaccgc
      541 gcagtcgccg cttttcgtga acccgcacag caccgccaag tgtccgtcgg agcgctccag
      601 cggagcaccc ggaagttcgc cctcgcgcca cgagtacgag atcgcgagcg gcaagttttg
      661 ctcgatcagc cgctgcgcgt ggtcgaggtt gcgcaggtgg gcgacgacgc cgcacaatcc
      721 gaggcttccg ctgtacgcga cgttgaaggt ccagtttccg gtaccgttgt acgcgcggtc
      781 gaacacggcg cgcgccgtcg tctcgaccgg aacgtcgatt ccgtggtacg cgtgaatcat
      841 cgcgaggctg gccggactgc accagccgcg ttcgccgtcg acggcgtact gcgaacggac
      901 cggtacgtcg aggatcagcg cgtcgcgcgc gtacggaagc gacggccgcg gacgcagcgg
      961 cgacgagaac gccacgaggt tgaagtctat tccggcggcg cgggcctcga ttccatcgaa
     1021 aggctgctcc gcgcggatca cgtcgacgtc gacgtgcacg ccttcgtggt cggggctgaa
     1081 cgacttcgaa ccgttgggat gccattcggc gtaatcgatc cagtcggtcg ccggccggcg
     1141 cgcgcgcagc aggcggaact cgatgcggcc ggcgggcgca aacgtattcc agctcagaac
     1201 gccgccgcga gccggctcga acagcaagcg cgcgctctcg tgtgcgtccc ggacttgcac
     1261 gaagcccgcg ggcgactcgt ctagacgctc cactgccacg agttccacgt atcgccgatg
     1321 aaggccgccg gaacgtagtt cttcacgtcg gcgttcatcg cggtatagct ttcttcccag
     1381 ctaaagaaga ccgccgggac gagttcccgg atgcgctcct cttcgcgctg atagagcgct
     1441 ttgcgcgcgc gccggtcgaa cgtgtgcgcg gccgccgcgc tggtggcgtt cacgattggg
     1501 tcgtcgagcc aggacgtatt cgccccgttg ggcgggatga aggcgccgtt ccaattgccg
     1561 gtgttgtcgg gatccggccc gttggtatcg atcgaccact cgaggtcgta cttgccggtg
     1621 tagagcgggc cgttctgggc gaacatctcg ttggcgggat agttgcgaac gcgcacgtcg
     1681 atgccgatcg cgcggagcat cgattggatc aggacttcgg actgttcgtt ttcctggtgc
     1741 ccgatggtgg ccgagatcgt cagctgcaga accgatccgc ccttgtgcag cgcgccgtcg
     1801 cctccgcgta tccaaccggc gcgcgccagc agcgcgcgcg cgccggcggg gtcgtaccgg
     1861 tagggcggca gggccggtgc ggcccacgac tccggaaaca cgtcggagac ggccagccgg
     1921 ttgaggccgt gatagacggt gtcgtctatg cgtttccagt cgacgccttc ggcgatcgcc
     1981 cgccgaacgg cgatgtcgcg cagcagcggg cggctcatgt tgatcccgag atggcgccag
     2041 ttggcggtga gctgcttctt gaccgcgatg ccggcgatgc cgcccagccg cgcgacggcg
     2101 ttggcgtcga cgttgggata gacgtcgatt tcgtgcgtct cgagctcgct gaagagcgtg
     2161 ttgacgtc
//
LOCUS       DASUCJ010001080         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_164192, whole genome shotgun sequence.
ACCESSION   DASUCJ010001080 DASUCJ010000000
VERSION     DASUCJ010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164192"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFU90_12735"
     CDS             <1..986
                     /locus_tag="VFU90_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480701.1"
                     /translation="LELLGVTHAKDEFVLPDAGERPLAVGAIVPLARAVDRAGNDVTT
                     VLADDDSLVFRSDPKTIGGVTATDFDDWIDLTIPVPPGRDSVVLVFRLRNSLLNTALL
                     YDEMLRPQGARAIDFEGRDLNTIADMVTLGRWYGSRMGLHVSVRRGGEWQEVVDVPDA
                     GPIAWRDVAAVIPAPKGDTLRVRLSFVADEWRLGKVSVATRFRRPVPQRFAPSTLVAA
                     NTAADTQALRSVAETDARYLETTPGQQFTIRFRTGAAPRDSVRTFLLATDGYYTEWIR
                     GAWVRQAAGAAPHPFIASDSTLAETVRHWSSVAPAFEAAFNRTRIPVRHVP"
BASE COUNT          167 a          354 c          341 g          181 t
ORIGIN      
        1 agctcgagct cctgggtgtg acgcacgcga aggacgaatt cgtcctgccg gatgccgggg
       61 agcgccccct ggccgttggc gcgatcgtac cgctggcgcg cgccgtcgat cgtgccggca
      121 acgacgtgac cacggtgctg gccgacgacg attcgttggt ctttcggtcg gatccgaaaa
      181 ccattggcgg cgtcaccgcg acggacttcg atgactggat cgacctgacc attcccgttc
      241 cgcccggccg cgacagcgtc gtcctcgtgt tccgtctccg caacagcctc ctgaataccg
      301 cgctgctgta cgacgagatg ctgcggccgc aaggcgcgcg cgccatcgat ttcgaaggac
      361 gcgacctcaa caccatcgcc gacatggtga cgctcggccg gtggtacgga tcgcggatgg
      421 gactgcacgt atcagttagg cggggaggcg aatggcaaga agtcgtcgac gtccccgatg
      481 cagggccgat cgcttggcgc gacgtcgcgg ccgtgattcc cgcgcccaag ggcgacaccc
      541 tccgcgtgcg gctgtccttc gtggccgatg aatggcgact cggcaaggtg agcgtcgcga
      601 cccgattccg ccgtcccgtg ccgcagcgat ttgcgccgtc gacgctcgtc gcggcaaaca
      661 ccgcggcaga tacgcaggcg ttgcggagtg tcgccgaaac ggatgcgcgg tatctggaaa
      721 cgacgcccgg ccaacagttc acgatccgtt tccgaaccgg cgccgcgccc cgcgactccg
      781 tccgcacgtt tctgttggca accgacggct actacacgga gtggattcgc ggcgcctggg
      841 ttaggcaggc ggccggagcc gcgccgcacc ccttcattgc ttccgattca acgctcgccg
      901 aaaccgtacg gcactggtcg agcgtggcgc cggcgttcga ggcggcgttc aatcgcaccc
      961 gcattcccgt gaggcacgtg ccatgagatg cacggtgatc attgccctga gcgctctcgc
     1021 gcttgcgtgc tacatcggac ctc
//
LOCUS       DASUCJ010001081         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_210425, whole genome shotgun sequence.
ACCESSION   DASUCJ010001081 DASUCJ010000000
VERSION     DASUCJ010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210425"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFU90_12740"
     CDS             complement(<1..825)
                     /locus_tag="VFU90_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU5480702.1"
                     /translation="MPAFVGNQSLLLDSRARPVAASLALAALGVVFGDIGTSPLYAFR
                     QCFTNLLHLAPTHENVLGVLSLILWSLILIVFVRYIGMVMHVAHDGEGGILALLALVL
                     PPVVRGVPPRATWLTFLILLGAGMLFGDGVITPAVSVLSSIEGLGVATSAAQPFVVPI
                     AVGVLLALFYFQKRGTGRIGSVFGPVMLLWFLTIAALGAGAIVAYPAVLLAIDPVYIA
                     RFFAHHGFAGISIFGAIVLCVSGVEALYADMSHFGRGPIALAWTAIVFPALALNYIG"
     gene            complement(789..1502)
                     /locus_tag="VFU90_12745"
     CDS             complement(789..1502)
                     /locus_tag="VFU90_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480703.1"
                     /translation="MLGLSHAILHVALCAPALRAKPETVVALRVRLLDHRLKPQLDRT
                     FRLERGDGDNPKIVEFDIAPGTYQLDAALPRYGCGVVDYLTFLPDHDRSIAETLSAGS
                     SPPPQPLLMNGASPQSFLYVQPTFVLLDKNATACNKPVGGLVPLRVTVENDQDAYYAA
                     LYPDPAAGTAPVQLALRLRTPTHQYHYVRVPIPFPMPWGGWPNNVRFDVTQDEIDGLA
                     SQPVDTLLCLHLWETKVYY"
     gene            1577..1948
                     /locus_tag="VFU90_12750"
     CDS             1577..1948
                     /locus_tag="VFU90_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5480704.1"
                     /translation="MSTATAGISDVAASYYLVKDLDRATEFYTRLFGAAPSMTMEGML
                     SEWSFPSGAAFGLYRPGDGSFRPYGGVMFNVPEVKAALEAAKAAGATFEDDDVTETPV
                     CFMAFGEDSEGNSFALHQPKT"
BASE COUNT          480 a          741 c          805 g          363 t
ORIGIN      
        1 tccgatgtag ttgagcgcga gcgcggggaa gacgattgcc gtccaggcca gcgcgatcgg
       61 tccgcggccg aagtgcgaca tgtcggcgta gagcgcttcg actccggaga cgcacagcac
      121 gatcgcgccg aagatactaa tccccgcgaa gccgtggtgc gcgaagaacc gcgcgatgta
      181 gaccggatcg atcgccagca gcacggccgg gtacgcgacg atcgcaccgg cgccgagcgc
      241 ggcgatcgtg agaaaccaca ggagcatgac cgggccgaac accgaaccga tgcggccggt
      301 tccgcgtttt tggaagtaga acaacgccag caaaacgccg accgcaatcg gcacgacgaa
      361 cggctgcgcg gcggacgtcg ccacgccgag cccctcgatc gacgagagca ccgaaacggc
      421 cggcgtgatg acgccgtcgc cgaacagcat cccggcgccg agaaggatga gaaacgtgag
      481 ccaggtagcg cgcggcggga cgccgcgcac gaccggcggc agcaccagcg cgagcagcgc
      541 cagaatcccg ccctcgccgt cgtgcgcgac gtgcatcacc atgccgatgt agcgcacgaa
      601 gacgatcagg atcagcgacc agagaatcag gctgagaacg ccgagcacgt tttcgtgggt
      661 cggtgccaag tgcaaaaggt tagtaaagca ctgccggaag gcgtacagcg ggctggtccc
      721 aatgtcgccg aacacgacgc ccagcgccgc cagcgcgagc gacgccgcga ccggccgcgc
      781 gcgggagtct agtagtagac tttggtttcc cacaaatgca ggcacaagag cgtgtcgacg
      841 ggctgcgatg cgaggccgtc gatttcgtct tgcgtgacgt cgaaccgcac gttgtttggc
      901 caaccgcccc acggcatcgg gaacgggatc ggcacccgca cgtaatggta ttgatgggtc
      961 ggcgttcgca gccgcagcgc cagctgaacg ggcgccgtac cggcggccgg atccggatag
     1021 agcgccgcgt agtaggcgtc ctggtcgttt tcgaccgtta cgcgcagcgg cacgagcccg
     1081 ccgaccggtt tgttgcacgc cgttgcgttc ttgtcgagca gaacgaaggt gggctgcacg
     1141 tataagaacg attgcggcga cgcgccgttc atcagcagcg gctggggcgg cggcgacgac
     1201 ccggcgctca gcgtttcggc gatgcttcgg tcgtgatcgg gtagaaacgt gagatagtcg
     1261 accacaccgc agccgtacct cggcaaggcc gcgtcgagct ggtaggttcc cggcgcgata
     1321 tcaaattcga cgatcttggg attgtcgccg tcgccgcgct ccaagcggaa ggtacggtcg
     1381 agctgcggct tcaggcggtg gtcgagcaag cgcacccgca gcgcgacgac cgtttcgggc
     1441 ttggcacgca gcgccggagc gcatagggcg acgtgcagta tggcgtgcga caggccgagc
     1501 atcgggccgg acttcaaggt gcgccgcgcc gctgcctgga agcgccccgt tccaatttca
     1561 cgatgaggaa gaattcatga gcacagcaac cgccggcatc agcgacgtcg ccgccagcta
     1621 ctatttggtg aaagacctcg accgcgcgac cgagttctac acgcggctgt tcggcgccgc
     1681 cccgtcgatg acgatggaag gcatgctttc ggagtggagc tttccgagcg gcgccgcctt
     1741 cgggctgtat cggcccggtg acggcagttt tcgtccgtac ggcggcgtga tgttcaacgt
     1801 gcccgaggtg aaagcggcgc tggaagcggc taaagccgcc ggcgccacgt tcgaggacga
     1861 cgacgtcacc gaaacgccgg tctgcttcat ggccttcgga gaagacagcg aaggcaattc
     1921 gttcgcgctg caccagccga aaacgtagca ggcggcccaa cgcgtcgctc gaaaacggcg
     1981 ccgttgggaa gcctggctcg ccaagtactt ataaagaaag ctcgaacgta cggagagccc
     2041 atgcgcgcgc cgcgcgccgt ccttcgacta cgctcgctca cgctcgctcc gctcgggatg
     2101 acaccgcccg acgattacgc tttggactta ataagcgagg acgcgggaac ggtcctgcga
     2161 cgggcgtctc gattttcggg caaacctgga tggtttcttc aaaactttgt tctcccgaaa
     2221 atcgagccga gcgagcggag acacatggac gttcggagcg agcgtcccgg aaccggagcg
     2281 ttacgcagac gagcaagtca gactgttgaa gcgactttga gcgcacgggg aagcgcgggg
     2341 cgagtagaac gctcgccccg cttcgcccgg atgaaacgcc gcgttttta
//
LOCUS       DASUCJ010001082         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_424012, whole genome shotgun sequence.
ACCESSION   DASUCJ010001082 DASUCJ010000000
VERSION     DASUCJ010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424012"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="rph"
                     /locus_tag="VFU90_12755"
     CDS             complement(<1..484)
                     /gene="rph"
                     /locus_tag="VFU90_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5480705.1"
                     /translation="MTIEPGFLKYAEGSALIGVGNTRVLCAATVEERVPPWLKGRGTG
                     WVTAEYAMLPRATHDRTQREATKGRLGGRTHEIQRIIGRALRAITDTAKLGERTLWID
                     CDVLQADGGTRTAAVTGAYVALALACAKLFDPRDAARWPLAGAVAAASVGIVNGQPML
                     D"
     gene            complement(584..2440)
                     /gene="glmS"
                     /locus_tag="VFU90_12760"
     CDS             complement(584..2440)
                     /gene="glmS"
                     /locus_tag="VFU90_12760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5480706.1"
                     /translation="MCGIVGYIGPRDSVPIILDALRRLEYRGYDSAGIAVLASLGALP
                     GSKAEGKLSRLAERLNNGEALSGGLGVGHTRWATHGKPSDANAHPHMDCQGKIAVVHN
                     GIIENYSPLRARLIEQGHAFTSETDTEVLAHLIESYYRAPGSEGGLVEAVRKTLHDVR
                     GAYALGVISSDDPEHLVFARNGASPLVIGIGKGEMYVASDTPAILPYTRKEVILQEGE
                     IAVVGRDGFTLMDYDGTRIERDPIQVMWDSTSAEKSGFKHFMLKEIFEQPNVIKETLA
                     GRNEPEGDLHLGRELGDVTASRLRELSKIAITGCGTAFHAGMVGMYLLRSLVRLPVEM
                     ELASEFRYGEPVIDPTTLVIAMSQSGETADTIEAVRIAREKGSAVLGVSNVVGSHLSR
                     ISDGTLYTRGGPEIGVAATKTYVSQVIAMTLFALHLAKLRGTSDPHRLHEIADGTRLL
                     PAAVDVVLNTSDEIRKVAREFRKAHSVLFLGRFVNFPTALEGALKLKEISYIHAEGYP
                     AGEMKHGPIALLDSSVPVVGIMTDDRVREKMLSNLMESRAREAPVIVVANHGDEEAKA
                     VADRVFWVPKVDELLSPIVNVIPLQLLAYHIADIEGKDVDQPRNLAKTVTVE"
     gene            complement(2453..2728)
                     /gene="rpoZ"
                     /locus_tag="VFU90_12765"
     CDS             complement(2453..2728)
                     /gene="rpoZ"
                     /locus_tag="VFU90_12765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5480707.1"
                     /translation="MQKKSVFGDLDVLLAHADSKFSLVNIVTKRARQLNNWIRVQQLP
                     PTDRREYFTSEPLVDKDTINPNKPVSIALDEIAEGKIAYHRTREGIK"
     gene            complement(2750..3370)
                     /gene="gmk"
                     /locus_tag="VFU90_12770"
     CDS             complement(2750..3370)
                     /gene="gmk"
                     /locus_tag="VFU90_12770"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5480708.1"
                     /translation="MITGPGLLFVVSGPSGAGKDTLVEALVESLPRLRYSISATTRAP
                     RPGERDGEHYFFVTRPEFESRRAAGALLETREYNEHLYGTPRDYIERSVAEGYDVIMK
                     PEVNGALAIKAAYPDAVLIFLVPDRFSHLRERLLARRTETNEEVARRLEIGHQEMTFI
                     RRFDYIVVNEQNRPEQAVLDLKAILHAERLKIHRYTDDSIKEVQTT"
     gene            3435..>3677
                     /locus_tag="VFU90_12775"
     CDS             3435..>3677
                     /locus_tag="VFU90_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480709.1"
                     /translation="MLTDWLLVRRLGAELEDRLRGGRVSAVVRLPDGRIGLGLRKAGA
                     DVTLAVDPFASPPLVTIEGGAPAGAEEPGFVRAAEGA"
BASE COUNT          572 a         1196 c         1199 g          710 t
ORIGIN      
        1 ggtcgagcat cggctgcccg ttgacgatcc cgacgctggc cgcggcgacg gcgccggcca
       61 gcggccaacg ggcggcgtcc cgcggatcga agagcttggc gcaggccagc gcgagcgcga
      121 cgtaggcgcc ggtcaccgcc gcggtgcgcg ttccgccgtc ggcttggagc acgtcgcagt
      181 cgatccagag cgtacgttcc ccgagtttcg ccgtgtcggt gatcgcgcgc agcgcgcggc
      241 cgatgatgcg ctgaatttcg tgcgttcgtc ctccgagtcg ccccttggtg gcttcgcgct
      301 gcgttcggtc gtgggtcgcg cgcggcagca tcgcgtactc cgccgtcacc caaccggtcc
      361 cgcgcccttt cagccacggc ggcacgcgct cttccacggt cgcggcgcac aggacgcgcg
      421 tgttcccaac gccgatgagc gcgctcccct cggcatactt caagaagccg ggttcgatgg
      481 tcaccggtcg aagctcgtcc ggccgacgcc cgtcgctgcg aatcataccg cttcgcagtt
      541 attgccgggc tgcgacgatt cctcgcccgg cggaccgggt acgctactct acggtgacgg
      601 tttttgccag gttccgcggc tgatcgacgt ccttgccctc gatgtccgcg atgtgatacg
      661 cgagcagctg cagcggaatc acgttgacga tcggcgagag cagctcgtcg actttcggta
      721 cccagaacac gcggtcggcg actgccttag cttcctcgtc gccgtgattg gcaacgacga
      781 tcaccggcgc ttcgcgcgcg cgcgattcca tcagattgga gagcattttc tcgcgcacgc
      841 ggtcgtcggt catgatgccc accaccggca cgctcgagtc gagcagtgcg atcggcccgt
      901 gcttcatttc gccggccgga tagccctcgg cgtgaatgta cgaaatttcc ttgagcttca
      961 aggcgccttc cagggcggtc ggaaagttca cgaaacggcc caggaagagc acgctgtggg
     1021 ccttgcggaa ctcgcgcgcc actttacgga tttcgtcgga cgtgttgaga accacgtcca
     1081 ccgcggccgg cagcagccgc gtgccgtcgg cgatttcgtg cagccggtgc ggatccgagg
     1141 tcccgcgcag tttcgccaaa tgcagtgcga acagcgtcat ggcgatgact tgcgacacgt
     1201 acgtcttagt cgcggcgacg ccgatctccg gtccgccgcg agtgtagagc gtcccgtcgg
     1261 agatccggct gaggtgcgag ccgaccacgt tgctgacgcc cagcaccgcg ctgcccttct
     1321 cgcgagcgat gcggacggct tcgatcgtgt cggcggtttc gcccgattgc gacatcgcga
     1381 tcaccagcgt cgtaggatcg attaccggtt cgccgtagcg gaattcgctg gcaagctcca
     1441 tttcgaccgg cagccgtacg agcgaacgca gcaggtacat ccccaccatg ccggcgtgaa
     1501 atgcggtacc gcagccggtg atcgcgatct tgctcagctc gcgcagccgg ctcgcggtta
     1561 cgtcgccgag ctcgcgcccg agatggaggt cgccttccgg ctcgttgcgc ccggcgagcg
     1621 tttccttgat gacgttcggc tgctcgaaaa tctccttgag catgaagtgc ttgaagccgc
     1681 tcttctccgc cgaagtagag tcccacatca cttgaatggg atcccgctcg atccgcgtgc
     1741 cgtcgtagtc catgagggta aagccgtcgc gtccgacgac cgcgatctcg ccttcttgga
     1801 ggatgacctc tttgcgcgta tacggcagga ttgccggcgt gtccgaagcc acgtacattt
     1861 cgcctttgcc gatgccgatg accagtgggc tcgcgccgtt gcgcgcgaac accagatgct
     1921 ccggatcgtc gctcgagatt acgcccagcg cgtatgcgcc gcgcacgtcg tgcagcgttt
     1981 tgcgcaccgc ctccaccaag ccgccttcgc tccccggggc ccggtagtac gactcgatca
     2041 gatgcgccaa gacttccgtg tcggtctcgc tggtgaacgc gtgaccttgc tcgatcaggc
     2101 gcgcgcgcag cggcgagtag ttctcgatga tcccgttgtg aaccaccgcg atcttcccct
     2161 gacagtccat gtggggatgg gcgttggcgt cggagggctt gccgtgggtc gcccagcggg
     2221 tgtgtccgac cccgaggccg cccgagagcg cttcgccgtt gttcaaccgc tccgcgaggc
     2281 gcgaaagctt accttcggcc ttcgatccgg ggagagcgcc cagcgaagcg agcacggcga
     2341 tgcccgcgct gtcgtatccg cggtactcca ggcggcgcaa ggcatcgaga atgatcggca
     2401 cgctgtcgcg gggcccgatg tatccgacga ttccgcacat ggccggccgg cgctacttga
     2461 tcccttcgcg cgtgcgatgg taggcgatct ttccctcggc gatctcgtcg agcgcgatcg
     2521 agaccggttt gttggggttg atcgtgtcct tatcgaccaa cggctcgctg gtgaagtatt
     2581 cgcgccggtc ggtcggcggc agctgctgca cccggatcca gttgttcagc tggcgggcgc
     2641 gcttggtgac gatgttgacg aggctgaact tcgaatcggc atgcgcgagc aggacgtcga
     2701 ggtcgccgaa gacggacttt ttctgcaaga tgattcctta tgtgggtggt taggtcgttt
     2761 gtacttcttt aatcgagtcg tcggtgtagc ggtgaatctt gagacgctcc gcgtggagga
     2821 tcgccttgag gtccaggact gcctgttccg gccggttttg ttcgttgacc acgatgtagt
     2881 cgaatcggcg aatgaacgtc atttcttgat ggccgatctc gagccggcgc gcgacctcct
     2941 cgttcgtctc ggtacgccga gcgagcagcc gttcgcgcag gtgcgagaac cggtccggaa
     3001 ccagaaagat caggaccgca tccggatacg ccgccttgat cgccagcgcc ccgttgactt
     3061 ccggcttcat gataacgtcg taaccttcgg ctacgctgcg ctcgatgtag tcgcgaggcg
     3121 tcccgtagag atgctcgtta tactcccggg tctcgagcag cgcaccggcc gcccggcggc
     3181 tttcgaactc cgggcgagta acgaagaaat aatgctcgcc gtcgcgctcg ccgggtcgcg
     3241 gagcccgggt cgtcgccgaa atcgagtacc gcagcctcgg caggctctcg accagggcct
     3301 cgaccagcgt atccttgccg gcccccgaag gacccgagac cacaaacagt agacccggcc
     3361 cggtgatcac gcgaactcca cggaaagctt agacagtgcg ctcaagttca caacggaggg
     3421 gtggttaacc tcgtttgctg accgattggc tgctggtacg ccgcctgggc gccgagctcg
     3481 aggaccggct tcgaggcgga cgggtgagcg ccgtcgtccg tctgccggac ggccggatcg
     3541 gcctcgggtt acggaaagcc ggggccgacg tcacgctcgc ggtggacccg tttgcctctc
     3601 cgccgctggt tacgatcgaa ggcggggcac cggccggcgc cgaggagccc gggttcgttc
     3661 gcgcggccga gggagcc
//
LOCUS       DASUCJ010001083         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1172978, whole genome shotgun sequence.
ACCESSION   DASUCJ010001083 DASUCJ010000000
VERSION     DASUCJ010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172978"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1106
                     /locus_tag="VFU90_12780"
     CDS             17..>1106
                     /locus_tag="VFU90_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEU5480710.1"
                     /translation="MNSSRGDFECAFNEYIAASGTFMDELADAISQTANFDLDRRTIT
                     PDSPYGTNTCDAQQQAGVYTPLSVARASNDVAVQHLEGWTDEQVPDRSHLIAVASAYG
                     GYSFVLMGEGMCSAAFDLGPEEMPPQIFADAMVRFDTAIAAATRASDTETLNLALLGR
                     ARTELDLGNSADAATDAALIPAGFEVDIDHDATATRRQNLVFIQTIQAQFASIDTSII
                     NRFTTDNDPRIAVTSTGALGSDGHTIVWFANKDATPTAPQALAKFSEAQLIIAENDVN
                     TGDLGDAVTILNQLREAAQQPDYSGGLTAPAVMADIVEQRRRELFLEGHRLGDIRRLG
                     LPLSPATGAPYVDGGAYADQSCFPLPNVE"
BASE COUNT          197 a          381 c          347 g          181 t
ORIGIN      
        1 gaacgcggac ttgatcgtga acagctcgcg cggcgacttc gaatgcgcgt tcaacgagta
       61 catcgcggcc agcggaacgt tcatggacga gctcgccgac gcgatctcgc agaccgcgaa
      121 tttcgatctc gatcgccgca cgatcacccc cgattccccc tatggaacga acacgtgcga
      181 cgcgcagcag caggccggcg tctacacgcc gctctcggtc gcgcgtgcat cgaacgatgt
      241 cgccgtgcag cacctcgagg gatggaccga cgaacaggtt cccgaccgct cgcatctcat
      301 cgccgttgcg tccgcgtacg gcggctacag cttcgtgctg atgggcgagg gcatgtgctc
      361 cgccgcgttc gatctcggcc ccgaggagat gccgccgcag atcttcgccg atgcgatggt
      421 tcgcttcgac accgccatcg ctgccgcgac gcgagccagc gatacagaga cgctcaatct
      481 cgcgctgctc ggtcgcgcac ggaccgagtt ggatctcggg aattcagcgg atgccgcgac
      541 cgatgcggcg ctcatccccg cgggcttcga agtcgacatc gatcacgatg cgaccgcgac
      601 gcggcgccag aacctcgtgt tcatccagac catccaggcg cagttcgcgt cgatcgacac
      661 ctcgatcatc aaccggttca ccacagacaa cgatccccgg atcgccgtga cgtcgaccgg
      721 ggcgttaggc tcggatggcc acaccatcgt ctggtttgcg aacaaggacg cgacgccaac
      781 ggctccgcag gcgctcgcca agttttcgga agcgcagctg atcatcgccg agaacgacgt
      841 caacaccggc gacttgggcg acgcggtcac gatcctcaac cagctgcgcg aggctgcgca
      901 gcagccggac tacagcggcg gcctgacggc tccggcggtg atggctgaca tcgtcgaaca
      961 gcggcgccgc gagttgttcc tcgaggggca ccggctgggc gacatccgcc ggctcggtct
     1021 cccgctgagt cctgcgactg gcgcgccgta cgtcgatggt ggcgcctatg cggaccagtc
     1081 gtgcttcccg ctgcctaacg tggagc
//
LOCUS       DASUCJ010001084         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_11627, whole genome shotgun sequence.
ACCESSION   DASUCJ010001084 DASUCJ010000000
VERSION     DASUCJ010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11627"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="VFU90_12785"
     CDS             <1..>1295
                     /locus_tag="VFU90_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEU5480711.1"
                     /translation="TTHSDTTSDTTQVPKDTIKAPLAHAEMPQLVGIGAQYHWDRDEM
                     FASGAINLLDLLKLIPGVTAFRSGWVTSPQYAAYLGNPGRVRYFYDGVEIENLDPRSP
                     GALDASEVQLFSLEEVSVERGADELRVYLRSWRVQRTSTNTRVDVLSGDEGTNLYRGF
                     YGKRYGNGMALQLAGQQYGTNSSPSIGGGDELALTGRLGWAKGPWSIDAYAIRSSRTR
                     DEQDQDLVSQVNGVVPEQDRTRTDAYLRAGYGDPDSGSWAQVMVATEQFAEHSGFHPQ
                     LDFAPADSADTTMSARQLVATGGFTRWGLRLSAADRLHFLPNSTMNSLEARLAYERKE
                     LAVSFFADYRGPDTTSTEEASARFTPLDFFSVTGAVTHRHGGGVDGGEQVAVRLEAGL
                     KVFNAWLTGGILRRDASLVPGLSAYDTSFVARRAIPATG"
BASE COUNT          216 a          416 c          448 g          215 t
ORIGIN      
        1 cacgacgcat tcggatacga cgagcgacac gacgcaggtg ccgaaggaca cgatcaaggc
       61 gccgctcgcg cacgccgaga tgccgcagct ggtgggcatt ggcgcgcagt accattggga
      121 tcgcgacgag atgttcgcgt cgggcgcgat caatctcctc gatctcctca agctgattcc
      181 gggcgtcacg gcgtttcgat ccggttgggt cacatcgccc cagtacgccg cgtacctcgg
      241 taatccgggt cgcgtgagat acttctacga cggcgtcgag atcgagaacc tcgacccgcg
      301 atcgccgggc gcgttggacg catccgaggt gcagctgttc tcgctcgagg aagtatcggt
      361 cgaacgcggc gccgacgagc tgcgcgtgta tctgcgcagt tggcgcgtgc agcgtacgtc
      421 gaccaacaca cgcgtcgatg tcctgtcggg tgacgaggga acgaatctct atcgcggctt
      481 ctacggcaag cgatacggaa acggcatggc gttgcagttg gcgggccagc agtacggtac
      541 caactccagt ccgagcattg gcggcggcga cgagctcgcg ctcaccggtc gcctcggttg
      601 ggcgaagggt ccgtggagca tcgacgcgta cgccattcgc tcatctcgca cgcgcgacga
      661 acaggaccag gacctcgtct cccaggtgaa cggtgtggtg cccgagcagg accgcacgcg
      721 caccgacgcg tatctgcgcg cgggctacgg cgatccggat tccggatcgt gggcgcaggt
      781 gatggtcgcc acggagcagt tcgcggagca ctcgggcttc cacccgcagc tcgacttcgc
      841 gcccgccgac tcggcggaca cgacgatgtc ggccaggcag ctcgtggcga cgggcggctt
      901 cacgcgatgg gggttgcgcc tgagtgcggc cgatcgcttg cacttcctgc ctaacagtac
      961 gatgaactcg ctcgaggcgc ggctcgcgta cgagcgcaag gaattggccg tgtcgttctt
     1021 cgccgactac cgcgggccgg acacgacgtc caccgaggaa gcatcggcgc gattcacgcc
     1081 gctcgacttt ttctcggtga cgggggcggt gacgcaccgg cacggcggcg gcgtggacgg
     1141 cggcgaacag gttgccgtca ggctcgaggc ggggctcaag gtgttcaacg cgtggctcac
     1201 cggcggcatt ctgcggcgtg acgcgagctt agtccccggt ctctccgcgt acgacacgag
     1261 cttcgtcgcg cgccgcgcca taccggcgac gggca
//
LOCUS       DASUCJ010001085         1322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1096747, whole genome shotgun sequence.
ACCESSION   DASUCJ010001085 DASUCJ010000000
VERSION     DASUCJ010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096747"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>1322
                     /gene="tolB"
                     /locus_tag="VFU90_12790"
     CDS             136..>1322
                     /gene="tolB"
                     /locus_tag="VFU90_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEU5480712.1"
                     /translation="MILSLRRWLAFGLLAAVPFVPAQAQLSIEIVGGAATAIPIAIVP
                     FDAEATWPLGITGIVGADLSRSGLFRLVNADGVVPRPARALDVQPAVWRGRGADAVVV
                     GSMRPLPDGRVDVRFDLVDVVKQATLASMAYTVAPAQFRATAHKIADVIYEKMTGAPG
                     VFSTRIAYVAKQGSRYQLMVADADGYNPQAIVSSNQPLLSPRWSPDGTRIAYVSFESR
                     KPVVYVQSLVNGTRRAIANFRGSNSSPGWSPDGSKLVVTLSKDGGSQMFLMNPDGTGV
                     TRVLTSASIDTEAMFTPDGRSLVFTSDRGGTPQIYRVSLATHQVERLTFEGDYNVSPR
                     PFPDGKGIAFVHRENGRFEIATLDFATHQMQVLTSGPSDESPSIAPNGRMIIYANEAG
                     GGGT"
BASE COUNT          201 a          451 c          438 g          232 t
ORIGIN      
        1 gaagcccgtc gcgtcgggga gcgcatcacg caggcgttcg gttatccggt catggtcgcg
       61 ccgaattgag catcgcctgc gcgtcgctat aatgcgcgag cgtcgtgatc gatccggcgc
      121 gctcgttcgc tcacgatgat cctttcgctt cgtcgctggc ttgccttcgg tttgctggcg
      181 gcggttccgt ttgtcccggc gcaggcccag ctctcgatcg agatcgtcgg tggggctgcg
      241 accgcgattc cgatcgcgat cgtcccgttc gacgccgagg cgacctggcc gctcggcatc
      301 accggcatcg tcggcgccga tctgtcgcgc tcgggtctgt tcaggctcgt caacgccgac
      361 ggcgtcgtcc cgcgaccggc gcgcgcgctc gacgtgcaac cggccgtctg gcgcggccgc
      421 ggtgccgatg cggtggtggt gggctcgatg cgcccgcttc ccgacggcag ggtcgacgtc
      481 aggttcgacc tcgtggacgt cgtgaagcag gcgacgctcg cgtcgatggc ctacacggtg
      541 gcccccgcgc agttccgcgc caccgcacac aagatcgccg acgtcatcta cgagaagatg
      601 accggcgcac ccggcgtgtt ctcgacgcgg atcgcctacg tcgccaagca ggggtcgcgc
      661 tatcagctga tggtcgccga tgccgacggc tacaacccgc aggcgatcgt gtcatcgaac
      721 cagccgcttc tgtcgccgcg ctggtctccc gacgggacgc gcatcgccta cgtttcgttc
      781 gagagcagga agccggtcgt ctacgtgcag tcgctcgtca acggcacccg gcgcgcgatc
      841 gccaattttc gcggcagcaa cagctcgccg ggctggtcgc cggatggcag caagctcgtc
      901 gtaacgctgt cgaaggacgg cggctcgcag atgttcctga tgaatcccga cggcaccggc
      961 gtcacgcgcg tgctcacttc agcttcgatc gacaccgagg cgatgttcac gcccgacgga
     1021 cggtcgctcg tgttcacctc ggatcgcggc ggcacgccgc agatctaccg cgtcagcctt
     1081 gcgacccacc aggtcgagcg gctcactttc gagggcgatt acaacgtgtc gccgcgaccg
     1141 ttcccggacg gcaagggcat cgcgttcgtg caccgcgaga acggacgctt cgagatcgcg
     1201 acgctcgact ttgcgacaca tcagatgcag gtgctgacgt cgggcccgtc ggacgaaagt
     1261 ccgagcatcg cgcccaacgg caggatgatc atctacgcga acgaagcggg aggcggcggc
     1321 ac
//
LOCUS       DASUCJ010001086         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_775661, whole genome shotgun sequence.
ACCESSION   DASUCJ010001086 DASUCJ010000000
VERSION     DASUCJ010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775661"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1144
                     /locus_tag="VFU90_12795"
     CDS             <1..>1144
                     /locus_tag="VFU90_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5480713.1"
                     /translation="LVEAPPRGLKILQRPRETTGPFSWITTVDHKKIAIMYGTLALCF
                     FVAGGIEALLIRVQLAQPNGTVLTAGQYNSLFTMHGTTMVFLLGMPIAAAFGNYLVPL
                     MIGARDVAFPRINMLGFWIFAFGGCFLYSSFFLGGAPNGGWFGYAPLTSTPMSNGYLP
                     GRGPDFWCVGLIMLGIGSVATAVNFLVTILNMRAPGMTMMRMPVFVWMTLVIAFLTLF
                     AMPPVTAALIMVFMDRNFHTNFFNAAAGGDPLMYQNLFWIFGHPEVYILILPGMGIVS
                     EILPVFSRKPLFGYAIVVFSGIAIGFLGWGVWAHHMFASGLGPVAVSAFGLSTMLIAI
                     PTGVKIFNWLGTVWGGAVRLATPMLFALGFIAQFTIGGLSGVMHSIV"
BASE COUNT          158 a          376 c          358 g          252 t
ORIGIN      
        1 cactggtcga agcgccgccg cgcgggttga agatcctgca gcgtccgcgc gagacgaccg
       61 ggccgttcag ctggatcacc acggtcgacc acaagaagat cgcgatcatg tacggcacgc
      121 tcgcgttgtg cttcttcgtc gcgggcggca tcgaagcgct gctgattcgc gtgcaactcg
      181 cgcagcccaa cggcaccgtg ctcaccgcag gtcagtacaa ctcgctcttc acgatgcacg
      241 gcaccacgat ggtgttcctg ctcggcatgc cgatcgcggc ggctttcggt aactacctcg
      301 tgccgttgat gatcggtgcg cgcgacgtcg cgttcccgcg catcaacatg ctcggctttt
      361 ggatcttcgc gttcggcggc tgcttcctgt actcgtcgtt cttcttgggc ggcgcgccca
      421 acggcggatg gttcgggtac gcgccgctca cgagcacgcc gatgtcgaac ggctatctgc
      481 cgggccgcgg ccccgacttc tggtgcgtcg ggttgatcat gctcggcatc ggttcggtcg
      541 cgaccgccgt caacttcttg gtgacgatcc tcaacatgcg cgcgccgggc atgaccatga
      601 tgcgcatgcc ggtgttcgtg tggatgacgc tcgtgatcgc gttcctcaca ctcttcgcga
      661 tgccgcccgt caccgcggcg ctgatcatgg tcttcatgga ccgcaacttc cacacgaact
      721 tcttcaacgc ggcggccggc ggcgatccgt tgatgtacca gaacctcttc tggatcttcg
      781 gccaccccga ggtgtacatc ctgatcctcc ccggcatggg catcgtctcc gagatcctgc
      841 cggtgttctc gcgcaagccg ctgttcggct acgcgatcgt cgtgttctcg ggaatcgcga
      901 tcgggttcct cggctggggc gtgtgggcgc accacatgtt cgcctcgggc ctcgggcccg
      961 tcgcggtgtc ggcgttcggt ctgtcgacga tgctcatcgc gatcccgacc ggcgtgaaga
     1021 tcttcaactg gctcggaacg gtctggggcg gcgcggtacg gcttgcgact ccgatgctgt
     1081 tcgcgctcgg tttcatcgca cagttcacga tcggtggatt gtcgggcgtc atgcactcga
     1141 tcgt
//
LOCUS       DASUCJ010001087         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1371690, whole genome shotgun sequence.
ACCESSION   DASUCJ010001087 DASUCJ010000000
VERSION     DASUCJ010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371690"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFU90_12800"
     CDS             complement(<1..987)
                     /locus_tag="VFU90_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480714.1"
                     /translation="MTRLSVGGFALAASLVLTSGTIRALEYPQPPADSTVDTYYGAQV
                     ADPYRPLESIDSPQTRAWVLAESSLSRSYLDAIPQRDSIKSHLQSIVNYERYGTPFHM
                     RDRYFYLYNSGLQNQSVLYTTRGLDGTPRVLIDPNRLSSDGSVTVGGESPSWNARYLA
                     YATQTSGSDWQTWHVRDVESATDLPDTLVWSKFAAAAWLPDDSGFFYERYPEPASGQT
                     YKGALYDQAVYLHRLGTPQGSDARFYYQPQHKNWIYGAAVTEDGRYLVLSVFSNRSVN
                     TRIGYVDLRDPKRSMHQLLWKNDAQWSFVGNAGPVFYFTTTLGSPNSRVVAID"
     gene            complement(1097..2119)
                     /locus_tag="VFU90_12805"
     CDS             complement(1097..2119)
                     /locus_tag="VFU90_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5480715.1"
                     /translation="MNGTVRLGYLPGIVILAGLILANSHGRVMGAQTPDGKAVFLVNC
                     AACHQPNGRGGGPFPPLAGNPAVNAVDSATLIQTVLNGRTGPITVNGVQYGGNMPSWR
                     ELSDADIAAVLTYVRSSWNNSAPAVSADQVAAARAPVAMSGEALFAARCATCHQPAGQ
                     GTDVFPPLAGNPVVSAADPGAMIAVIVNGRSGPLTVNGKTYSGQMPTWKGQLSNADIA
                     SVATYVRSAWGNNASPVTEEEVAAAGTPVSVQVGASVYAKNCAACHASNGAGGVGPAL
                     AGNPRVNIDSPVPMLTMIVQGRNLMPSWRGQLSAGDIAAVATYIRSSWGNSVPAVSVQ
                     QVMAIK"
     gene            complement(2180..3355)
                     /locus_tag="VFU90_12810"
     CDS             complement(2180..3355)
                     /locus_tag="VFU90_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480716.1"
                     /translation="MKDYRRKGVVSCVGIAAFAAIAIATNKPAQAMPNFAQAYGIPCS
                     YCHIQIPALNAYGRWVQRTGYAALNPHVLKRESPIWVDYPVSYSQQSPNAGSWDIGSL
                     GVHADGAFGTPSSEWTYHVQQWIWQSSQAGGLDTAWIAYNNFFSGAGHIFAGKVEAPG
                     PSEFSMWSDVSGLTANSSAEITVGEHTYQLDANRWGYKFAYERGSLDAQVAYLTSGAD
                     LNGFNAYDWGQDKTLQYKLAYANRSNPLEVGYYGARGSWPLSAGGFDQYYSNAFYAQR
                     DPVNGIPGFLATYQMNHDGNPGVGLPPAGSNGVSYEIYDTIGKRALLSAGEQLTNDGL
                     GGHTQIGNIDASYHLTRFITVYAEEALGVGQKPTWNGLIWFALPTGPLWPPLSQTGW"
     gene            complement(3470..3826)
                     /locus_tag="VFU90_12815"
     CDS             complement(3470..3826)
                     /locus_tag="VFU90_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5480717.1"
                     /translation="MTRSFRNLAIIVAAAWALLPAAGVSANPTVNVVASNWKFTPSTI
                     ELHVGQTTTLRLTSSEGVHGLQSDELGIPLTTIAPGAAKAVTVTAKKPGTYVLHCAIM
                     CGAGHANMTLTVNVVP"
     gene            complement(3936..>4161)
                     /locus_tag="VFU90_12820"
     CDS             complement(3936..>4161)
                     /locus_tag="VFU90_12820"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480718.1"
                     /translation="VIAGYPRLKKIVWVQEEPKNMGARAFVRRRLLESKRDGFDIEYI
                     GRGYRASPSEGYAGQHAVEQERIVTTALAE"
BASE COUNT          743 a         1307 c         1325 g          786 t
ORIGIN      
        1 gtcgatcgcg acgacgcgcg agttcgggga gcccagcgtc gtcgtaaaat aaaaaaccgg
       61 tccggcgttg ccgacgaacg accactgcgc gtcattcttc caaagcagct ggtgcatcga
      121 acgtttcgga tcgcgcagat cgacgtatcc gatccgcgtg tttaccgaac ggttcgagaa
      181 gaccgaaagc acgagatacc ggccgtcctc ggtgacggcg gcgccgtaga tccagttctt
      241 gtgctgcggc tggtagtaga agcgcgcgtc cgaaccctgc ggcgttccca gcctgtgcag
      301 gtacaccgct tggtcgtaga gcgcgccttt gtaggtctgc cccgacgccg gctcgggata
      361 gcgttcgtag aagaatccgc tgtcgtcggg cagccaggcg gcggccgcga acttgctcca
      421 tacgagcgtg tccggcaggt cggtggccga ctcgacgtcg cgcacgtgcc aggtttgcca
      481 gtccgagccc gacgtctgag tcgcgtacgc gaggtagcgg gcgttccagc tgggcgactc
      541 gccgccgacc gtcaccgacc cgtcgctcga aagacggttg ggatcgatca ggactcgcgg
      601 cgtaccgtcg agcccgcgcg tcgtatagag caccgactga ttctgcaagc cgctgttgta
      661 gagatagaag taccggtcgc gcatgtggaa cggcgtaccg taccgttcgt agttgacgat
      721 cgattgcagg tgcgacttga tcgagtcgcg ctgcggaatc gcgtcgaggt acgatcgcga
      781 cagcgacgat tcggcgagca cccacgcgcg cgtttgcggc gaatcgatgc tctcgagcgg
      841 ccggtacgga tcggcgacct gcgcgccgta gtacgtgtcg accgtcgaat cggccggcgg
      901 ctgcggatat tccagcgctc ggatcgttcc ggacgtcagc acgagcgatg cggccagcgc
      961 gaagcccccg accgaaagac gcgtcacgat gtacctccaa aaagatcgag agacgccggt
     1021 gcgtccctcg gttttttcgc ggttccgccc ttccccgcgt tcgaacggat gccgctcgca
     1081 gccggccggg cgaggcttac ttgatcgcca tgacctgttg gacgctcacg gcgggaacgc
     1141 tgttgcccca cgacgaccgg atgtaggttg cgacggcggc gatgtcgccg gccgagagct
     1201 gcccgcgcca cgaaggcatc agatttcggc cctgaacgat catcgtcagc atcggaaccg
     1261 gactgtcgat atttacgcgc ggatttccgg cgagtgccgg cccgaccccg ccggctccgt
     1321 tggaggcatg gcaagcggcg caattctttg cgtataccga agccccgacc tgaacggata
     1381 cgggtgtacc cgcggctgca acttcctctt ccgtcaccgg cgacgcgttg ttcccccatg
     1441 cggatcgaac gtaggtcgca accgaggcga tgtcggcgtt gctgagctgt cccttccacg
     1501 tcggcatttg accgctgtac gtcttaccgt tgaccgtgag cgggccggag cggccgttaa
     1561 cgatcaccgc gatcatggca ccgggatcgg cagcactgac gaccgggttg cccgccagcg
     1621 gcgggaaaac gtccgtcccc tggcccgcgg gttgatggca ggtcgcgcag cgggcggcaa
     1681 aaagcgcctc gccgctcatc gcaaccggtg cccgggcggc cgcaacctga tcggcggaca
     1741 cggccggcgc gctgttgttc caagaggaac gcacgtacgt cagcaccgcc gcgatgtcgg
     1801 cgtcggaaag ctcgcgccaa gacggcatgt tgccgccgta ctgcacgccg ttgaccgtga
     1861 tcggcccggt gcgcccgttc aataccgtct ggattaacgt cgcgctatcg acggcgttga
     1921 ccgctgggtt acccgcgagc ggcgggaaag gtccgccgcc ccggccgttg ggttggtggc
     1981 acgccgcgca gttgacgagg aacaccgcct tgccgtcggg cgtttgagcg cccatcaccc
     2041 taccgtggga attcgcgaga atcaatcccg cgaggatgac gattccggga agataaccga
     2101 gtctaacggt cccgttcaag cggacctcct ggaagcggcg ggtactccgc tttaggaggc
     2161 tcgctttcga cggttcaaat caccaacctg tttgggaaag cggcggccac aggggacccg
     2221 tcggcaaggc aaaccagatc agtccgttcc acgtgggttt ctgtccgacg cccagcgcct
     2281 cttcggcgta gaccgtaatg aagcgcgtaa ggtggtaaga ggcgtcgatg ttgccgatct
     2341 gcgtgtgccc gcccaatccg tcgttggtga gttgctcgcc ggcggaaagc agcgcacgct
     2401 ttccgatggt gtcgtagatc tcgtacgaaa cgccgttgct gcccgccggg ggcaacccca
     2461 cacccgggtt gccgtcgtga ttcatctggt aggtcgcgag gaaaccgggt atgccgttga
     2521 ccgggtcgcg ctgcgcgtaa aacgcattgc tgtaatactg gtcgaatccg cctgcgctca
     2581 acggccagga gccgcgcgcc ccatagtacc cgacctcgag cgggttgctg cggttcgcgt
     2641 acgcgagctt atactgcagc gtcttgtcct gcccccaatc gtaggcgttg aatccgttga
     2701 ggtcggcacc cgaggtcaaa tacgcgactt gcgcgtcgag ggagccgcgt tcgtaggcaa
     2761 acttgtaccc ccagcggttg gcgtccagct ggtaggtgtg ctcgccgacc gtgatctcag
     2821 ccgatgagtt ggcggtcagg ccgctgacgt cggaccacat gctgaactcc gagggccccg
     2881 gcgcctcgac cttcccggcg aaaatgtggc ccgcaccgct gaagaagtta ttgtaggcga
     2941 tccaggccgt gtcgaggccc cccgcttggc tcgactgcca aatccattgc tgcacgtgat
     3001 acgtccattc gctgctcggc gttccgaacg cgccgtcggc gtgcacgccc agacttccga
     3061 tgtcccacga cccggcgttc ggcgattgct gactgtagct cacggggtaa tcgacccaga
     3121 tgggagactc gcgtttcaac acgtgcgggt ttagcgccgc atatcccgtc cgctgaaccc
     3181 agcgcccgta cgcgttcagc gcgggaatct gaatgtgaca gtatgaacac gggatgccgt
     3241 acgcttgcgc gaagttgggc atcgcttgcg ccggtttatt cgtcgcgatc gcgatcgcag
     3301 cgaaagcggc gataccaaca cacgagacta cgccttttcg ccgatagtcc ttcataacgg
     3361 tgtctccatt ccaagatcga cgccggaatt aagccgctcg ggcctttctc gtatcgtcgg
     3421 cagagtcttt tgcaaccgcg taaacctcgt tcttttagga acgggacgtt tacgggacga
     3481 cgttaaccgt gagcgtcatg ttggcgtgtc cggcgccgca catgatcgcg caatgaagca
     3541 cgtacgtgcc cggcttcttc gccgtcacgg taacggcctt agccgcgccc ggggcgatgg
     3601 tcgtcagcgg aatgccgagc tcgtcggatt gcagcccgtg cacgccttcg ctcgaggtga
     3661 ggcgtaaggt cgtggtctgc ccgacgtgga gctcgatagt cgaaggagtg aatttccagt
     3721 tcgaagcgac gacgttgacg gtgggattgg cactgacccc cgccgcgggc aacagtgccc
     3781 acgctgccgc gacaattatc gcaagatttc taaaagaacg cgtcatgttt aaccttcctc
     3841 tcttcggaaa ggttagccca acgagaggaa tcgccggtga acgaagccgc ccgcaatgag
     3901 aagaactgga gaatctctcc tccgagacgg ggggattact cggccagagc ggtggtgacg
     3961 atgcgctcct gctcgaccgc gtgctgtccg gcgtacccct cgctgggact cgcacggtag
     4021 ccgcgtccga tgtactcgat atcgaagccg tcgcgtttgc tttcgagcaa gcgccggcgc
     4081 acgaaggcac gcgctcccat gttcttgggc tcttcctgca cccagacgat cttcttcagc
     4141 cgcggatagc cggcgataac g
//
LOCUS       DASUCJ010001088         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_515834, whole genome shotgun sequence.
ACCESSION   DASUCJ010001088 DASUCJ010000000
VERSION     DASUCJ010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515834"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFU90_12825"
     CDS             <1..788
                     /locus_tag="VFU90_12825"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEU5480719.1"
                     /translation="RNPGDAALSADGSRVYVPNQGSNDVSAIDTASDRVIATVKVGKI
                     PATLRLSPDGKTAWVTNYGDNTIQAIDLATLRAGKPIGVGHAPQQLAIAPDGGTLYVA
                     DQNDNTVTPVDVRSGKALPPIPVGAKPGGIAISHDGRTAYVTNQGDNSITPIDLASRT
                     ARPAFPAGVQPQGMAMSADDSLLYVADAGSDTLAVIDARSGARTRTIRAGLGPVAVAL
                     SADGRELYAVLLGDNACVKISIDDPRADSVVEMGNAPLAIALP"
     gene            824..1195
                     /locus_tag="VFU90_12830"
     CDS             824..1195
                     /locus_tag="VFU90_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480720.1"
                     /translation="MHRLRAILAGACALIAGIVFLASAHGSGAAAQPRSCGMATYSSA
                     GVIYRVLFAKNGAVQQYLLAESSHNTEKDHDVLKALEQKYGPEAVNAPPLQITSFKPG
                     TGGMMVPDKAIDSCGRVTHFH"
BASE COUNT          261 a          440 c          430 g          215 t
ORIGIN      
        1 gcaggaatcc cggcgatgcc gcgctcagtg cggacggcag ccgcgtctac gttccgaatc
       61 aagggagcaa cgacgtaagc gcgatcgaca cggcatccga tcgcgtcatc gcgacggtaa
      121 aggtcggcaa gattccggcg acgcttcgcc tctcaccgga cggcaagacg gcatgggtta
      181 ccaactacgg cgataacacg atccaggcga tcgatctggc aacgctgcgc gccggtaaac
      241 ccatcggcgt cggacacgcg ccgcaacaac tggcgatcgc tccggacggc ggcacgctgt
      301 acgtcgccga tcagaacgac aacaccgtaa cgccggtcga cgtacgcagc ggcaaagcgc
      361 tcccgccgat tccggtcggc gcaaagccgg gcggcatcgc gatctcgcac gacggccgga
      421 cggcgtacgt cacgaaccag ggcgataaca gcatcacgcc gatcgatctc gcatcacgaa
      481 cggcgcgtcc ggcgtttccg gccggcgtgc agccgcaggg catggcaatg agtgccgacg
      541 attcgctcct ctacgtcgcg gatgccggtt ccgacacgct cgccgtcatc gatgcgcgaa
      601 gcggcgctcg cacgcggacc attcgtgccg gcctgggtcc ggtcgccgtc gcgctttccg
      661 cggatgggcg cgaactctat gccgtactgc tcggcgacaa cgcatgcgta aagatttcga
      721 tcgacgatcc gcgcgctgac agcgtcgtgg aaatggggaa tgcgccgctg gcgattgcgc
      781 tcccgtaacg ccgcggtgac gaacggccga tacaacggag tagatgcatc gacttcgcgc
      841 gatcctggcc ggcgcgtgcg cgcttatagc cggcatagtc tttctcgcct ccgcgcacgg
      901 cagcggcgcc gcggcgcagc cgcgttcgtg cggcatggcg acgtattcca gcgccggagt
      961 gatctaccgt gtgctgttcg cgaagaacgg ggcggtacag caatacttgc ttgcggagag
     1021 ctcgcacaac accgagaaag atcacgacgt cctcaaagcg ctcgaacaga agtacggtcc
     1081 cgaagccgtc aatgcgccgc cgctgcagat cacgagcttc aagcccggaa cgggcggaat
     1141 gatggttccg gataaagcga tcgactcgtg cggccgcgtc acgcactttc attaggaacg
     1201 gcgctccggc cgagcctccg cgccccccat cggagatggg gccccattgg gcccggtcgt
     1261 cggtaaagca aaagccgcgg ttacgaccgc ggcttttgtt tcttgcaagg ggtcagtgta
     1321 aacgttcgtt cgctctcgcg aagact
//
LOCUS       DASUCJ010001089         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_592450, whole genome shotgun sequence.
ACCESSION   DASUCJ010001089 DASUCJ010000000
VERSION     DASUCJ010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592450"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFU90_12835"
     CDS             complement(<1..1003)
                     /locus_tag="VFU90_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU5480721.1"
                     /translation="MLSLAFSAAMLGIEGYVVRVEAASSAGIPHFSIIGLPDRALREA
                     CDRVRTAIFNSGFTYPAGRLLVNLAPADVRKAGPAFDLAIALALTAIDDQVDRLALQE
                     FIALGELTLDGALRGVNGILPMVLGARNAGFRKFVVPEANADEAALVDGIELYAVDTL
                     GSAALVLAGNGARWRRKTVPPVLESAGDRVHGDLADVRGQVAAKRALEVAAAGGHNLL
                     LVGPPGCGKTMLARRLPSILPPLTLPEALDVTKIYSVAGFLLRDAGIVRARPFRFPHH
                     TISQTALVGGGALAKPGEISLAHHGVLFLDELPEFSRSAIEVMRQPLEEGSVTIARAA
                     "
     gene            complement(1052..2392)
                     /locus_tag="VFU90_12840"
     CDS             complement(1052..2392)
                     /locus_tag="VFU90_12840"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480722.1"
                     /translation="MKRFVRTAAVYAAILALAACSASSGTLSPSLQSGRVLQSIDGSG
                     AGKIRHVVWVVQENRSFDDMFEGYPGADTVSSGKDSRGNTIALQPVSLKTAYEIDHSA
                     FAMFDACNGTGKLPGTQCRMNGFNLERQFGGPPNGQYVYVPHTESKPYWDMAHEWVLA
                     DRMFASQLDESFVAHQYIVAAQAQSSVDVPSFYWGCEGGKTDVVEILKRNRTYGGVQR
                     PCFDYETLGDELDKAGLTWRFYTSGYHVPYSGLWSGYQAVRHIFRGPDWKTDVITPQK
                     SFLTDVAAGKLASFTWITPLCHESDHSDCGGGLGPSWVSSIVNAVGKSKFWDSTAIFV
                     QWDDWGGFYDHVAPPKADYDGLGFRVPLIVISPYAKKNYVSHVRYETASVLRFAEDLF
                     GLPQLADADGRAASPAADCFDFARPPRKFVPIQAPLGPDFFRRDAGDDRIPDEQ"
BASE COUNT          444 a          807 c          799 g          419 t
ORIGIN      
        1 ccgccgcgcg cgcgatggtg accgaccctt cttcgagcgg ttgccgcatg acttcgatcg
       61 cgctgcgcga gaactcgggc aattcgtcga gaaacagcac gccgtggtgc gcgagcgaaa
      121 tttcacccgg tttcgcgagc gcgccgccgc ccaccagcgc ggtctgcgag atcgtgtggt
      181 gcggaaaacg gaacggtcgc gcgcgcacga tgccggcgtc gcgcagcaga aagcccgcga
      241 cgctgtaaat tttcgtcacg tcgagcgcct cgggcagcgt cagcggcgga aggatcgacg
      301 gaaggcgtcg cgcgagcatc gtctttccgc agccgggcgg accgaccagc agcaggttgt
      361 ggccgccggc ggcggcgact tccagcgcgc gcttggctgc gacttgcccg cgcacgtcgg
      421 cgagatcgcc gtgaacgcga tcgccggccg attccaaaac cggcggcacg gttttccgcc
      481 gccaccgcgc cccgtttccg gccaagacta acgccgccga tccgagcgtg tcgaccgcat
      541 acagctcgat tccgtcgacc agcgcggctt catcggcgtt ggcttcgggg acgacgaact
      601 tgcgaaagcc ggcgttacgc gcgccgagca ccatcggcaa gattccgttg acgccgcgca
      661 gcgcgccgtc gagcgtcaac tcgcccagtg cgatgaactc ttgcagcgcg agccggtcga
      721 cttgatcgtc gatcgcggtc agcgccagcg cgatcgcgag atcgaaggcg ggcccggcct
      781 tgcgcacgtc ggcgggggcg agattgacca gcaaccgtcc cgccgggtag gtgaaaccgg
      841 aattgaagat cgcggtgcgc acgcggtcgc aagcttcgcg gagcgcccga tccgggagac
      901 cgatgatcga aaaatgcgga atccccgcgg agctggccgc ttcgacgcgc acgacgtagc
      961 cttcgatgcc cagcatcgcc gccgaaaacg caagcgacag catccgcgcc acctctgcgc
     1021 accggacggg ctccggctag gggccggcgc tctactgctc gtcggggatg cggtcgtcgc
     1081 cggcgtcgcg gcggaagaag tccgggccga gcggggcttg gatcgggacg aacttgcgcg
     1141 gcggccgggc gaaatcgaaa cagtcggccg ccggcgaggc cgcgcgcccg tcggcatccg
     1201 ccagctgcgg cagcccgaag agatcctccg cgaagcgcag cacgcttgcc gtttcgtacc
     1261 gtacgtgcga gacgtaattt ttcttggcgt acggcgaaat cacgatcagc ggtacccgga
     1321 atcccaaccc gtcgtagtct gctttcggcg gcgctacgtg atcgtagaag ccgccccaat
     1381 cgtcccactg cacgaagatc gcggtcgaat cccagaactt gcttttgccg acggcgttga
     1441 cgatggagct cacccaggac ggtccaaggc cgcctccgca gtccgaatga tcggattcgt
     1501 gacagagcgg cgttatccac gtgaagctcg cgagcttgcc ggcggcgacg tcggtgagaa
     1561 agctcttttg cggtgtgatg acgtccgttt tccaatcggg tccgcgaaag atgtggcgga
     1621 ccgcctgata tccggaccat agcccgctgt acgggacgtg atagccgctc gtatagaagc
     1681 gccacgtcag gccggcttta tcgagctcgt ctccgagcgt ttcgtagtcg aagcaaggcc
     1741 gctgcacgcc gccgtacgtg cgattccgct tgagaatttc gaccacgtcg gttttcccgc
     1801 cctcgcatcc ccaataaaac gacggaacgt cgacgctcga ctgcgcctgt gccgcgacga
     1861 tgtactggtg tgcgacgaag ctttcgtcga gctgcgaagc gaacatccgg tcggctaaga
     1921 cccactcgtg cgccatgtcc cagtacggct tcgactcggt atgcggcacg taaacgtact
     1981 gtccgttcgg cggtcctccg aactgacgct cgagattaaa accgttcatc cggcactgcg
     2041 tgcccggaag tttaccggtg ccgttgcacg cgtcgaacat ggcgaaggcc gagtgatcga
     2101 tttcgtaggc cgtcttgagc gaaacgggct gtagcgcgat ggtgtttccg cgcgagtctt
     2161 tgccgctcga gaccgtatcg gcgccgggat atccttcgaa catatcgtcg aagctgcggt
     2221 tctcctggac gacccaaacg acgtgccgaa ttttgccggc gccgctgccg tcgatcgatt
     2281 gcagaacgcg tccgctttgc agcgacggtg aaagcgtgcc ggacgatgcc gaacaggccg
     2341 ccagagccaa gatggcggcg tagacggccg ccgttcgaac gaaccgcttc acgaccttga
     2401 aaaagtgccg cacggcgagg cgtcatccct ccgcccggcg acgcagctcg cccgctttca
     2461 cgattctga
//
LOCUS       DASUCJ010001090         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_470155, whole genome shotgun sequence.
ACCESSION   DASUCJ010001090 DASUCJ010000000
VERSION     DASUCJ010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470155"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFU90_12845"
     CDS             <1..984
                     /locus_tag="VFU90_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5480723.1"
                     /translation="VRNAILLAVAYLVLAVVTAVQPIPIASARVAVPFPGGAPLMFGL
                     PNRPFTVLVIGLDRRPSDTGPSRTDTILLVHIDPNAHRASFLSIPRDSMMEVPSPDGS
                     WTQDRINTAYVYNWSKKDAAAAPQALMRTIEHNLGIKVDHYVTWDQYSAESLIDAMGG
                     VTIDNPKAFGQSDYSDDDIHVKPQQFPVGTQHLDGYQAVAYGRIRQGSSDFDRIMRQQ
                     RVAAGLIDEAASPLSLLKVPAVWRAYEKTIGTDMSLRQSAGVLAMLKRVPDSKLKTKS
                     LGDAATPCTSCSASIQLLVPSKVQEIVADALDSPAAGQRAAELLQADGVAG"
BASE COUNT          198 a          410 c          372 g          177 t
ORIGIN      
        1 gtccgcaacg ccatcctgct tgcggtcgcg tatctcgtgc tcgcggtagt gacggccgtg
       61 cagccgatac cgatcgcgtc ggcccgagtc gccgtcccgt tcccgggcgg cgcgccactg
      121 atgttcgggc taccgaaccg cccgttcacg gtgctcgtca tcgggctcga ccgccgaccg
      181 tccgacaccg gcccgagccg cacagatacg atcctcctcg tgcacataga cccgaacgca
      241 catcgcgcgt cgttcctcag catcccgcgc gactcgatga tggaggtgcc ttcgccggac
      301 ggcagctgga cgcaggaccg catcaacacc gcgtacgtct ataactggtc gaagaaggac
      361 gctgccgccg caccgcaggc cctcatgcgg acgatcgagc acaacctcgg catcaaggtc
      421 gaccattacg tcacgtggga ccagtacagc gccgagagcc tgatcgacgc gatgggcggc
      481 gtcaccatcg acaacccgaa ggcgttcggg cagtccgact actccgacga cgacatccac
      541 gtgaagccgc agcagttccc ggtcgggacg cagcatctcg acggctacca ggccgtcgcg
      601 tacgggcgca tccggcaggg ttcgtcggac ttcgaccgca tcatgcgcca gcagcgcgtc
      661 gccgcgggtc ttatcgacga agcggcgtcg ccgctgtcgc tgctgaaggt gcccgcggtg
      721 tggcgcgcgt acgagaagac cattggcacg gacatgagcc tgcgccagtc ggcgggcgtg
      781 ctcgcgatgc tgaagcgcgt cccagactcg aagctcaaga caaagtcact cggcgacgcg
      841 gccacgccct gcacgtcgtg ctccgcgtcg atccaactgc tcgtgccgtc gaaggtgcag
      901 gagatcgtcg ctgacgcgct cgacagcccg gcggcgggcc agcgcgcggc ggagttgttg
      961 caggcggacg gggtagccgg atagcggcga gcgctgcggg cctaagcccg gaccttcccc
     1021 gcgacgccca cgaatgcctc gaacaatcgg cgcatgcgcg gcgcgaagtc cggttcgtgc
     1081 tcttccggcc gctcgggatg ccactgcacg ccgatcatcc acggataccg atcgctctcg
     1141 tacgcctcga tcagcgt
//
LOCUS       DASUCJ010001091         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1249666, whole genome shotgun sequence.
ACCESSION   DASUCJ010001091 DASUCJ010000000
VERSION     DASUCJ010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249666"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFU90_12850"
     CDS             <1..260
                     /locus_tag="VFU90_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HEU5480724.1"
                     /translation="MVCMGVLVLRRREPHLHRPFKTPGMPWVPILGALICFAQMIGLP
                     LETWARLVIWLVVGLVIYFWYGRHHAQRIRDAAPTAAIAAD"
     gene            497..1159
                     /locus_tag="VFU90_12855"
     CDS             497..1159
                     /locus_tag="VFU90_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5480725.1"
                     /translation="MGARTTSGTITVDGGENGGARVAGWDRDSIDVIAHIETHARSDE
                     EAQAMAKQISIDMSNGHIHAEGPRTHDGASWSVSFDVLVPRASDLSITATNGPVAIQS
                     VHGKIELSAVNGPMDIDDAGGDVHGRTTNGPLSVRLTGTTWVGRGLDAVTTNGPATIS
                     IPEPYSAHLETGTSNGPMSIGIPITVQGQILRHLETDLGSGGPTVRAVTTNGPLVIER
                     AD"
     gene            complement(1167..>1721)
                     /locus_tag="VFU90_12860"
     CDS             complement(1167..>1721)
                     /locus_tag="VFU90_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5480726.1"
                     /translation="GTGLGLSTVYGIVSQSAGHIVVTSTEGGGSTFTVYLPRVKEGEN
                     EQETSTASVVPSSGHETILFVEDDPAVRTLVVRVLEAHGYHVIAAADAKEALAAAAAH
                     AGDIHLLVTDVVMPGWNGRELADRLSDSRPGIHVLYLSGYADDALLRRGVTARHTWLL
                     QKPFDTAALLGKIQEALGTSSAAR"
BASE COUNT          283 a          564 c          603 g          271 t
ORIGIN      
        1 tgatggtgtg catgggcgtg ttggtgctgc ggcgtcgcga accgcatctg caccggccgt
       61 tcaagacgcc gggcatgccg tgggttccga tactcggcgc gctgatctgt tttgcacaga
      121 tgatcggatt gccgctcgag acgtgggcgc ggctggtgat ctggctggtg gtgggtctcg
      181 tgatctattt ctggtacggc cggcaccacg cgcagcgtat tcgcgacgcg gccccaaccg
      241 cggcaatcgc ggccgactga gggcacaaac caattccgga ttggcgctgt cgaacttcga
      301 tggccggccc agcgccggac atttcaacgg tgccaatccg gaggctcgca tgcgtcgctg
      361 cctgtctgct gttgctgcgc tgatcgcgtt cgtgtgggcg gcgcccgttg cccatgccca
      421 gcaccgcgag cgcaactggc tcgagcgctg ccgcgaggcg gacacggacc gcccgcgcca
      481 ttgcgaggag cgcacgatgg gcgcgcgtac aaccagcggg acgatcaccg tggatggcgg
      541 agagaacggc ggggcgcgcg tggccggttg ggatcgcgac agcatcgacg tcatcgcgca
      601 catcgagacg catgcgcgca gcgacgagga ggcgcaggcg atggccaagc agatctcgat
      661 cgacatgtcg aacggccaca tccacgccga gggaccgcgc acgcacgatg gcgcgtcctg
      721 gtcggtgtcg ttcgacgtgc tggtgccgcg cgcctccgac ctctccatca ccgcgacgaa
      781 cggaccggtg gcgatccagt cggtgcacgg caagatcgag ctgagcgcag tgaacggacc
      841 gatggacatc gacgacgcgg gcggcgacgt gcacggccgc acgacgaatg ggcccctctc
      901 ggtgcgtctc accggcacga cgtgggttgg gcggggactc gatgcggtaa cgaccaacgg
      961 tccggcgacg atcagcattc ccgagccgta ctcggcgcat ctcgagaccg ggacatcgaa
     1021 cggaccgatg tcgatcggaa tcccgatcac ggttcaagga cagatcctgc gccacctcga
     1081 gaccgacctg ggttcgggcg gtccgacggt ccgcgcggtg acgaccaacg gtccgttagt
     1141 catcgagcgc gccgactgac cgcgcgtcag cgggcagccg acgacgtgcc gagcgcttcc
     1201 tggatcttgc cgagcagggc ggccgtgtcg aacggcttct ggaggagcca cgtgtggcgc
     1261 gcggtcacgc cgcggcggag gagcgcatcg tcggcatagc ccgacagata gagcacgtgg
     1321 atgcccggcc gcgagtcgga cagtctatcg gccagctcgc ggccgttcca gcccggcatc
     1381 acgacgtccg tgacgagaag atgaatgtca ccggcgtgcg ccgcggccgc ggccaacgct
     1441 tccttcgcgt cggccgctgc gatgacgtga tagccgtgcg cttcgagcac gcgcacgacg
     1501 agcgtccgca cggcgggatc gtcctcgacg aacaggatcg tctcgtgccc gctggacgga
     1561 acgaccgacg cggtcgacgt ctcctgctcg ttttcaccct ccttgacgcg cggcaggtag
     1621 accgtgaacg tgctgccgcc gccctcggtg ctcgtgacca cgatgtgtcc ggcgctctgt
     1681 gacacgatgc catacacggt cgagagcccg aggccggtgc c
//
LOCUS       DASUCJ010001092         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_592463, whole genome shotgun sequence.
ACCESSION   DASUCJ010001092 DASUCJ010000000
VERSION     DASUCJ010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592463"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..>1179)
                     /locus_tag="VFU90_12865"
     CDS             complement(96..>1179)
                     /locus_tag="VFU90_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU5480727.1"
                     /translation="GALVLRDPSGLVNPFYKLVPAPLLYVVVAIATVATIIASQALIS
                     GIFTLAKQAIALGFIPRARVIYTSAIHQGQVYVPALNVLLAIACIALVVGFRSSARLA
                     DAYGLAVAVTMVVTTLAYYVVVRERFGWAAWRAAFATVPFLVIELLFVAGSLRKLFEG
                     AYIPVVISLAVFAIASTWRAGRRRVALSYVEQSEPVEKFLAEVKDRLGTPLHGTAVFL
                     TADPEGVPFVMRHHWAQTHSVDERIVLLTILPANDPYVDDFHRVTVERLADSLIRVTA
                     RFGFMEKLGIDAIVKGCAEAGLYLGSSDTTYYSADPQIVPRSRGRWNAWRRNLYVFLK
                     RNARPMTSSLGIPADALAKLGLEVPM"
BASE COUNT          233 a          373 c          398 g          175 t
ORIGIN      
        1 agcggacgcg cgagcgtgcc gcaatagtaa tcgggcgcgt cccggcacgc gtgcagcgcg
       61 agcgtcccga cgagcatcgc agccgggagc acgtgctaca tcgggacttc gagcccgagc
      121 ttggccaagg cgtcggccgg aatgcccaga ctcgaggtca tcggacgcgc gttgcgtttg
      181 agaaagacgt agagattgcg ccgccacgcg ttccaacggc cgcggctgcg cggcacgatt
      241 tgcggatccg cggagtagta cgtcgtatcg ctgctgccga ggtagagccc ggcttcggcg
      301 cagcctttca cgatcgcgtc gatcccgagc ttctccatga agccgaaccg ggcggtgacg
      361 cggatcaaac tgtcggctag ccgctcgacc gtaacgcggt ggaagtcgtc gacgtacgga
      421 tcgttggcgg gaaggatcgt gagcagcacg atgcgctcgt cgacgctgtg ggtctgcgcc
      481 cagtgatggc gcatcacgaa cggaacgccc tccggatctg cggtgagaaa aacggccgtg
      541 ccgtgcagcg gcgttcccag tcggtctttg acctcggcaa gaaacttttc aaccggttcg
      601 gattgctcga cgtagctgag cgccacgcgc cggcgtcccg ctcgccaggt gctcgcaatc
      661 gcaaagacgg ccagactgat gaccaccggg atgtacgctc cttcgaagag tttgcgcagg
      721 ctgccggcga cgaacagcag ctcgatcacc aagaacggga cggtggcaaa cgcggcgcgc
      781 caggcggccc agccgaaacg ctcgcgcacg accacgtagt acgcgagggt cgtgaccacc
      841 atggtgaccg cgacggcaag cccgtaggcg tcggccagcc gcgccgagct gcggaaaccg
      901 accaccagcg cgatgcaggc gatcgccagc aatacgttca gcgccggcac gtagacttgg
      961 ccttggtgga tcgccgacgt gtagatcacg cgggcgcgcg ggatgaaccc cagcgcgatc
     1021 gcctgcttcg caagcgtaaa gatccccgag atcaaggctt gcgacgcgat gatcgtcgcg
     1081 acggtcgcga ttgcgaccac cacgtagagc agcggcgcgg ggacgagttt ataaaacgga
     1141 ttgaccaatc cggaagggtc gcgcaagacc agcgcgccc
//
LOCUS       DASUCJ010001093         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1035607, whole genome shotgun sequence.
ACCESSION   DASUCJ010001093 DASUCJ010000000
VERSION     DASUCJ010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035607"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..>1254
                     /locus_tag="VFU90_12870"
     CDS             250..>1254
                     /locus_tag="VFU90_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480728.1"
                     /translation="MALIVAGLAASSGNASSRSTHLLTGINDEAFTLYGDPASAFETL
                     TSLRTQVLRVNLYWGGNQWAVANTEPSVATDPGDPAYNWSLYDRLARYASNSGIKLLF
                     SIVGTPRWANHGAGKNHAPTSFTTLQQFAQAAAKRYSGSYVAPAAQADTTLAPADSPL
                     PAVKMWTAWNEPNNPVFLAPQYKRVHGAWRVESAYQYARICNAIYAGIHAGASGEKVA
                     CGVTDPRGNDNAGSSRASVDPLSFLIAAHRYGMKRFDVYAHNPYASADNESPGYVPRG
                     RASRRIQMGNIGLLLSEISRFYGPKHLWITEYGYQTNPPDHTILGVSWAKQAAYLKQA
                     Y"
BASE COUNT          224 a          423 c          417 g          190 t
ORIGIN      
        1 tccggaaatg gtggagcgat gggcgttccg agcacctcgt gccggtctgg caccggcatc
       61 ggctcgctgc ggccggggag cgcgtcctcc ggcgtgacca tgtgcgctgg cttcgtgaaa
      121 aggaacatgg tggaaagggt acggatttac gcaagtcgag gtttgaacgt tttgtgacgg
      181 gtaggcgggg acggaacccg aacccgaggg ggtcttgacg tgcacctgag aatcgcgcgc
      241 gccgctgtcg tggcgctcat cgtcgccggc ctggcggcat cctccggaaa cgcctcgtcg
      301 cggtcgacgc atctgctgac gggcatcaac gacgaggcgt tcacgctgta cggcgatccc
      361 gcctcggcgt tcgagacgct gacgtcgctc aggacgcagg tcctgcgcgt caacctctat
      421 tggggcggca accaatgggc agtcgcgaac acggagccgt cggttgcgac cgatccgggc
      481 gaccccgcgt acaactggtc gctctacgac cgcctggcgc gctacgcctc gaactcgggc
      541 atcaagctgc tgttctccat cgtcggcacg ccgaggtggg cgaaccacgg cgccgggaag
      601 aatcacgcgc cgacgagctt caccacgctg cagcagttcg cgcaggccgc cgcgaagcgg
      661 tacagcggct cgtacgtcgc gcccgcagca caggccgaca cgacgctcgc gccggcggac
      721 tcgccgcttc ccgcagtgaa gatgtggacg gcctggaacg agccgaacaa ccccgtcttc
      781 ctcgcgccgc agtacaagcg tgtgcacgga gcgtggcgcg tcgagagcgc gtaccagtac
      841 gcgcgcatct gcaacgcgat ctatgccggg atccacgccg gagcatcggg cgagaaggtc
      901 gcgtgcggcg tcaccgatcc gcgcggcaac gacaacgccg gctcgagtcg agcttccgtc
      961 gacccgctca gcttcctcat cgccgcgcac cgctacggga tgaagcgctt cgacgtctac
     1021 gcgcacaacc cgtacgcgtc ggccgacaac gaatcgccgg gctacgtgcc gaggggccga
     1081 gccagccgcc gcatccagat gggcaacatc gggctcctcc tgtcggagat ctcgcgcttc
     1141 tatggaccga agcacctgtg gatcacggag tacggctacc agacgaatcc gccggatcac
     1201 accatcctcg gcgtcagctg ggcgaagcag gcggcgtacc tcaagcaggc atac
//
LOCUS       DASUCJ010001094         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1310488, whole genome shotgun sequence.
ACCESSION   DASUCJ010001094 DASUCJ010000000
VERSION     DASUCJ010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310488"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFU90_12875"
     CDS             <1..949
                     /locus_tag="VFU90_12875"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5480729.1"
                     /translation="HMRVVAADVGVAGLLRRLGLKSFGKRFTPMIYRNVPRSMIAWDS
                     VSPIREANPTQVSLSVKESKLARLHPSELAEIISDLSAREAAAVVGQLDDETAADAFE
                     HLEPEKQKTLIGDIGTERAADIIEEMDADDAADLLSELPEEQQSQLLAEMSDYTADEL
                     RELVKYADDTAGGLMTTDYVWIYPHRTTEATIRKIREIAPASEFIYYLYVLDKADHLL
                     GTLSLRTLLLELPTAFIDRIMETDMITVAPDAPAVDVAATIARYDLLAVPVVDDRGKM
                     LGIVTVDDAIDAIMPEEVAKKLPRFTPRRHSPPAVAAGA"
     gene            953..2239
                     /locus_tag="VFU90_12880"
     CDS             953..2239
                     /locus_tag="VFU90_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU5480730.1"
                     /translation="MRLSRAHRRSLMRSLLILGAVVGPGFITASAGNDVGGIFQYSQA
                     GAQFGYSLLWLLFPLAIALIVVQEMATRMGCVTGKGLAALFRENFGVRTSFLAMLLLF
                     LINTLITATEFAGVAAAAQILFHVSRYVSVPIALVAVFFFVLRFDNKIVERTFVVFSL
                     IYLTYIASAVLAHPNWSEVAHGAFVPSFRLQTAGWISAVVGLIGTTISPYMQFFLQSA
                     VVEKGSRSEGLVLARIDVINGSILAIALAGFMIVANAATIYLANQHGAHIVATQASDF
                     AVALRPLAGEAASIIFALGILNAGIFTATVLPLSTSYIVCEAFGFEAAIDRKFHEAPV
                     FFSLFAAGLVIGAAVVLIPGLPLLNMVLFAQVLQGILLPAELVLMLIVINSKQIMGEY
                     TNKPLGNAIAWATVVVVGTLSVLYVAGQFFPKLLGS"
BASE COUNT          372 a          766 c          699 g          439 t
ORIGIN      
        1 gcacatgcgc gtggtcgcgg ccgatgtcgg cgtcgccggc ctgctgcgcc gcttaggcct
       61 caagtcgttc ggcaaacgct ttacgccgat gatctatcgc aacgtgccgc gctcgatgat
      121 cgcgtgggat tcggtctcgc cgattcgcga agccaatcct acgcaggtga gtctttcggt
      181 caaggaaagc aaactcgcgc ggctgcaccc gtcggagctt gccgagatca tcagcgatct
      241 ttcggcgcgc gaagccgccg ccgtggtcgg ccagctcgac gacgagaccg cagccgacgc
      301 gttcgaacac ctcgagccgg aaaaacaaaa gacgctgatc ggcgacatcg gcacggagcg
      361 cgccgccgac atcatcgaag agatggacgc cgacgacgcg gccgatctgc tctccgagct
      421 gcccgaggaa cagcagtcgc agctgctcgc cgagatgagc gactacaccg ccgacgagct
      481 gcgcgagctc gtcaagtatg ccgacgacac cgcgggcggc ttgatgacga ccgactacgt
      541 atggatttat ccgcaccgca ccaccgaggc gacgattcgc aagatccgcg agatcgctcc
      601 ggcctcggaa ttcatctact acctctacgt gctcgacaaa gccgaccatt tgctgggaac
      661 gctctcgctg cgcaccctgc tgttggagct gccgacggcg ttcatcgacc ggatcatgga
      721 aaccgacatg ataacggtcg cgcccgacgc gccggcggtc gacgtcgcgg cgacgatcgc
      781 gcgctacgac ctgctggcgg tgccggtggt cgacgatcgc ggcaagatgc tcggcatcgt
      841 caccgtcgac gacgcgatcg acgcgattat gcccgaggag gtcgccaaga aactgccgcg
      901 ctttacgccg cggcggcatt cgcccccggc cgtcgccgcc ggagcctaaa acgtgcgttt
      961 gagccgtgcg caccggcgct cgttgatgcg atcgctgctg atcctcggcg ccgtcgtggg
     1021 gcccggcttc atcacggctt ccgccggcaa cgacgtcggc ggcatcttcc agtattcgca
     1081 agccggcgcg cagttcggct acagcctgct ctggctgctg tttccgctgg cgatcgcgct
     1141 gatcgtcgtg caggagatgg cgacgcggat gggatgcgtc accggcaaag gcctcgccgc
     1201 gctgtttcgc gagaacttcg gcgtgcggac gtcgtttctc gcgatgctgc tgctgtttct
     1261 catcaacacg ctgatcaccg ccaccgaatt cgccggcgtg gccgcggccg cccaaatact
     1321 cttccacgtt tcgcgctacg tctcggttcc gatcgcgctg gttgcggtct tcttcttcgt
     1381 gctgcgcttc gacaacaaaa tcgtcgagcg cacgttcgtg gttttctcgc tgatctacct
     1441 cacctacatc gcctcggccg tgctcgcgca tccgaactgg agcgaagtcg cgcacggcgc
     1501 gttcgtgccg tcgttccgcc ttcagaccgc cggctggatc tcggcggtcg tcggcttgat
     1561 cggcacgacg atctcgccgt acatgcagtt ctttctgcag tcggcggtgg tcgagaaggg
     1621 atcgcgctcg gaagggctgg tgctggcgcg catcgacgtc atcaacggct cgatcctcgc
     1681 aatcgcactc gccggcttca tgatcgtcgc caacgccgcc accatctatc tcgccaacca
     1741 gcacggcgcc cacatcgtcg cgacgcaggc gtcggacttt gcggtcgcac tgcgtccgct
     1801 cgccggcgaa gccgcctcga tcatcttcgc gctcggcatc ctcaacgccg gaatcttcac
     1861 cgcgaccgtg ctcccgctct cgaccagtta tatcgtgtgc gaagcgttcg gcttcgaagc
     1921 cgccatcgac cggaagtttc acgaagcccc ggtcttcttc tcgctgtttg ccgccgggct
     1981 ggtgatcggg gcggccgtcg tcctgatccc ggggctgccg ctgctgaaca tggtgctgtt
     2041 cgcgcaggtc ctgcagggca tactgctgcc ggccgagctc gtgctcatgc tgatcgtcat
     2101 caacagcaag caaatcatgg gggagtacac gaacaagccg ctggggaatg cgatagcctg
     2161 ggccaccgtg gtcgtcgtcg ggacgctctc ggtgctctac gtcgccgggc agttctttcc
     2221 gaagttgctg ggatcgtgaa ctccgaacgc atcgtcgctt ttgccgagac actggc
//
LOCUS       DASUCJ010001095         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_592530, whole genome shotgun sequence.
ACCESSION   DASUCJ010001095 DASUCJ010000000
VERSION     DASUCJ010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592530"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          203 a          417 c          376 g          187 t
ORIGIN      
        1 agggagcgat gcgccgccgt catctgccga gggtcgaagc gctctccgct cgccgtgcgg
       61 tggccagcga gcttcgcccc gtaccaggtt gcgtcgccga cgagaacttc ggaccgggga
      121 gcacgctcgg cggatgcggc gcgcgtttcg gcgtgcgaag ggggctctgt cgcgtcttct
      181 acggtgttgc ggcgcgcaca cgccgaggcg cagacggcca cgacgaggag cgcacgggct
      241 acggcgcgcg gcatcaacaa aggctctcag ccttacgcgg atgcgcgccc gtcgcaacac
      301 cgttgctcgc ggcgccgata cacttacagc caggccaatt tcgtgcacga gtcctccttc
      361 gtggctcgca ttcgactcgc gtcgctaccc gtcgatggcg actccgaagt gggcgacgcc
      421 gccctcgcgg aggcgagcgc gttcgtccgc aattctgctt gtagctacgt cgcaaaggtc
      481 aaaaacgtat tgaccggatt gccgcccacg gacgccgaag ttcgatcgga ggtgacggcg
      541 gccgatccgg cgacgcagct ccaaacgctg attaagggct ggatgctcct ccctgatcct
      601 gcgtcgacca gctcgccaag cgctcgtctt cggagggcag acgcagcccc tcattccgcc
      661 gacggcgctc aaggccacgc tgacgaaccc gggccctctt acgaatctgc aatcgcttcg
      721 cgatcagacg atgaattcgc tttacgatct gtaccgaaac gaagctaccc ccgggcaaaa
      781 ggcgtacgtc gacgcgctgg ccacgtccca acagctccgg cggcgaatac gcggccgtcg
      841 ccatcgactc gaagagcgcc ctcggttcca cgagcggcga cgtcgcgccc gcgacgtccc
      901 tcgcttcgtg gggccggacg atgatggccg tcgtagggat cgatgccgct accatcgcgc
      961 aggccatccc gagcgggacg acgatcacgg gagcgctcac cacgtgacgc gcggccgagg
     1021 acgcgcttca gtgcgcgtcc gcccaactct tgccccagcc cacgtccacg acgagaggca
     1081 cggccagctt catcgcgccg gccatctgcg tgcgaatgcg ctcgcccgct tcggccgcgt
     1141 gaacctcggg cacctcgaat acgagctcgt cgtgaaccgt aag
//
LOCUS       DASUCJ010001096         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_363311, whole genome shotgun sequence.
ACCESSION   DASUCJ010001096 DASUCJ010000000
VERSION     DASUCJ010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363311"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VFU90_12885"
     CDS             <1..1537
                     /locus_tag="VFU90_12885"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480731.1"
                     /translation="LSVITVRTPEPTFDAMLNRWTLYQALSCRMWARSALYQSGGAYG
                     FRDQLQDVMAFVYAAPELAREHILRAAARQFVEGDVQHWWHPGSGRGVRTRFSDDMAW
                     LPYVVDHYVRVTGDGSVLDAPAPFLTMRALEAGEHEAYEVPQVSGETGTVYEHCVRAL
                     RKACTTGAHGLPLIGGGAWNDGMNRVGVDGRGESVWLAWFLIATLRAFAPVAAARGDA
                     AIADEFLTQAGAYARAVESDGWDGEWYRRAYFDDGTPLGSALGDECRIDALAQSWSVI
                     SGAGDPQRQSRAMRSLEAHLVRDDARLILLLAPPFNRTPHDPGYIKGYLPGVRENGGQ
                     YTHAALWAALATARRGDGDRAFEMFQMLNPLTRSRTPGDVERYKVEPYVVAADIYAAE
                     GHVGRGGWTWYTGSASWMYRVGVEAILGVVKQGAMLSIHPCIPRDWRQYEIRYRYSRS
                     LYIIAVHVTDDIAAAVAISVDGKLLDTPAIPLVDDGAQHDVTVQLGRAGAAIVSEKQQ
                     GTQ"
     gene            1534..>2415
                     /locus_tag="VFU90_12890"
     CDS             1534..>2415
                     /locus_tag="VFU90_12890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5480732.1"
                     /translation="MKKIGFLSFGHWTPSRPSVARSARDVLQQSIELAVAAEQLGADG
                     AYFRVHHFARQLGSPFPLLAAVGARTSRIEIGTAVIDMRYENPFYMVENAGAADLISN
                     GRLQLGISRGSPEQVIEGWRHFGYAPAEGGTDADLGRRHAETFLALLTGDGFARPNPR
                     PMFPDPPGLLRLEPYSAGLRERIWWGSASNATAIWAAQHGMNMQSSTLKADESGEPFH
                     VQQARQIRIFRQAWSEAGHTREPRVSVSRSIFALMNDRDHAYFGRDERSDQIGIIDNM
                     RAVFGRSYAAEPDVLVEE"
BASE COUNT          389 a          762 c          854 g          410 t
ORIGIN      
        1 gctttcggtg atcaccgtgc gcacaccgga accgaccttc gatgcgatgc tcaaccgatg
       61 gacgttgtac caggcgctct cgtgtcgcat gtgggcccgg tcggcgctgt atcagagcgg
      121 cggagcctac ggatttcgcg accagctgca ggacgtgatg gcgttcgtct atgcggcgcc
      181 ggagctggcg cgggaacaca tccttcgcgc cgcggcgcgc cagttcgtcg agggcgacgt
      241 gcagcactgg tggcacccgg gcagcggacg cggtgtgcga acgcggttct ccgacgacat
      301 ggcgtggctg ccgtacgtcg tcgatcacta cgtgcgcgtc accggcgatg gatcggtgct
      361 cgatgcacca gcgccgtttc tcacgatgcg agcgctcgag gcgggcgagc acgaggcgta
      421 cgaggtgccg caggtgtcgg gggagaccgg aacggtgtac gagcactgcg tgcgcgcctt
      481 acggaaggcg tgcacgaccg gcgcgcatgg actgccgttg atcgggggcg gcgcttggaa
      541 cgacggcatg aaccgtgtgg gggtggacgg gcggggcgag agcgtctggc tggcgtggtt
      601 tctcatcgcc acgctgcgtg cgtttgcgcc ggtcgccgcc gcgcggggcg acgctgcgat
      661 cgctgacgaa ttcctgacgc aggccggcgc gtacgcgcgc gccgtcgagt cggacgggtg
      721 ggacggcgag tggtaccgcc gcgcctactt cgacgatggc acgcccctgg gctccgcgtt
      781 aggcgacgag tgccgcatcg atgcgttggc ccagagctgg agcgtgatct cgggcgccgg
      841 cgatccgcag cggcaatcgc gcgcgatgcg ctcgctggag gcgcatctgg tgcgcgacga
      901 tgcgcgcctc attctgctgc tcgctccgcc gttcaatcgc acgccgcacg atcccggcta
      961 catcaagggt tacctgccgg gggtccgcga aaacggcggc cagtatacgc acgcggcgct
     1021 gtgggcggcg ctcgcgacgg cgcggcgcgg tgacggcgac cgcgcgttcg agatgttcca
     1081 gatgctcaac ccactgaccc gctcgcggac gccgggtgac gtcgagcggt acaaggtcga
     1141 gccctacgtc gtggccgccg atatctacgc ggccgaggga cacgtcgggc gaggcggatg
     1201 gacgtggtac accggctccg cgagctggat gtatcgcgtg ggtgtcgagg cgattctcgg
     1261 tgtcgtgaag cagggtgcga tgctctcgat acacccgtgc attccgcgcg attggcggca
     1321 gtatgaaatt cggtatcgct acagccggag tctgtacatc atcgccgtgc atgtgactga
     1381 cgacatcgcg gccgccgttg ccatcagcgt cgacggcaag ttgctcgaca caccggcgat
     1441 cccgttggtc gacgatggcg cgcagcacga cgtgacagtg cagctcggcc gcgccggggc
     1501 cgctatcgtg tcggaaaaac agcaagggac gcaatgaaaa agatcggatt cctgtccttc
     1561 ggtcactgga caccgtcgcg cccatcggtc gcgcgatcgg cgcgcgatgt gcttcagcag
     1621 tcgatcgagc tggctgtggc ggccgaacaa ctcggcgcgg acggcgcgta ctttcgcgtg
     1681 caccactttg cccggcagct cgggtctccc tttccgcttc tcgcggccgt cggcgccagg
     1741 acgagccgca tcgaaatcgg aacggccgtc atcgacatgc ggtacgagaa tccgttctac
     1801 atggtcgaga acgcgggcgc ggccgatctg atcagcaacg gtcggctcca gctcggcatc
     1861 agccggggtt cgcccgaaca ggtcatcgaa ggctggcgtc acttcggcta cgcgccggcg
     1921 gaaggcggaa ccgacgcgga cctgggccgt cgtcacgccg aaacgtttct cgcgcttctc
     1981 accggcgatg ggttcgcccg gcctaacccg aggccgatgt ttccggatcc accgggcctg
     2041 ctgcgactcg agccgtattc ggcgggattg cgcgagcgca tctggtgggg ctccgcgtcc
     2101 aacgcgacgg cgatctgggc ggcgcagcac gggatgaaca tgcaaagctc caccctcaaa
     2161 gcggacgagt cgggcgagcc ctttcacgtc cagcaggctc gccagatccg cattttccgg
     2221 caagcgtgga gcgaggccgg gcatacccgg gagccccgcg tctcggtgtc tcgatccatc
     2281 ttcgcgttga tgaacgatcg ggaccacgcc tacttcggtc gcgatgaaag gagcgatcag
     2341 atcggaatca tcgacaacat gcgagcggtg tttggccgct cctatgctgc cgagccggac
     2401 gtattggtcg aagaa
//
LOCUS       DASUCJ010001097         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_699072, whole genome shotgun sequence.
ACCESSION   DASUCJ010001097 DASUCJ010000000
VERSION     DASUCJ010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699072"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFU90_12895"
     CDS             complement(<1..267)
                     /locus_tag="VFU90_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5480733.1"
                     /translation="MNDDAAEPLGVSIRRDVGVVVVAAGASTRVGGPELKQLRWLAGK
                     PMLLHSVETFMSRSDVVSVVVVLPPRYAGDPPPWLFQVDVDRLMV"
     gene            complement(264..>1274)
                     /gene="radA"
                     /locus_tag="VFU90_12900"
     CDS             complement(264..>1274)
                     /gene="radA"
                     /locus_tag="VFU90_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5480734.1"
                     /translation="LYVSGEESPLQVKLRADRLGSDAGSVELLSETNLETIIATAAAA
                     TPSALIVDSIQTIFTGDLEGAPGNVGQVRECAARLMRFAKESGTTVFVVGHVTKGGGI
                     AGPKTLEHIVDTVLYFEGESTLDHRVLRATKNRFGSVDEIGVFRMSESGLEPVENPSE
                     LFLGDRNMQASGSAVTALLEGTRPVLIEIQALAAKAGFGTPQRVSTGYDARRLALLLA
                     VLDKRAGLSFAALDVFLNVVGGVRLQEPAGDLAVAAALASTVYDRALPSDAVFVGEVG
                     LGGEVRPVSQAERRVAEAVNMGMARVFLADRGVPKRGTANRDAQIVGVRTIGELFQYL
                     FA"
BASE COUNT          237 a          450 c          390 g          197 t
ORIGIN      
        1 gaccatcagt cggtcgacat cgacctggaa caaccagggc ggcggatcgc ctgcatagcg
       61 tggcggaagc accacgacca ccgacacgac atccgagcgc gacatgaagg tctcgacgct
      121 atgcagtaac atcggcttgc ccgcgagcca gcgcagctgc ttcagctctg ggcctccgac
      181 gcgcgtgctc gccccggcag ccacgaccac gactcccacg tcgcggcgga tagaaacgcc
      241 taacggttcc gcggcatcgt cgttcacgcg aagagatatt gaaagagctc gccgatcgtc
      301 cgcacgccga cgatctgggc gtcccggttc gccgtgccgc gcttcggaac gccgcggtcg
      361 gcgaggaaca cccgcgccat gcccatgttg accgcttcgg ccacgcggcg ttccgcctgt
      421 gagacaggac ggacctcacc gcccagcccg acctcgccga cgaacacggc gtcggacggg
      481 agcgcgcgat cgtagacggt ggacgccaag gctgcggcca cagcgaggtc tcccgccggc
      541 tcctggagtc gaacgccacc caccacattc aggaacacat cgagcgcggc gaaggaaagc
      601 cccgcacgct tgtcgagaac cgcgagcagc agcgcgagac gacgcgcgtc gtacccggtc
      661 gagacgcgct gcggtgtgcc gaagcccgcc ttcgccgcga gcgcctgtat ctcgatcaat
      721 accggccggg tcccctcgag cagcgcggta accgcgctgc cggatgcttg catgtttcgg
      781 tcgccaagga agagctccga tggattctcc accggctcga gcccactctc cgacatgcgg
      841 aagacgccga tctcatcgac ggagccgaag cgattcttcg tcgcacgcag cacccgatga
      901 tcgagcgtac tctcgccctc gaagtacaac accgtgtcga cgatgtgctc gagcgttttg
      961 ggaccggcta tgccgccgcc tttcgtaacg tgtcccacaa cgaacacggt tgtgccactc
     1021 tccttggcga agcgcatcag ccgtgcggcg cattcgcgca cctgacccac gttgcccggc
     1081 gcaccctcga ggtcgccggt gaagatcgtc tggatggaat cgacgatcaa cgccgacggc
     1141 gttgcagcgg ccgcggtggc gatgatcgtc tcgaggttcg tctcgctcaa cagttccacg
     1201 ctgcccgcat cacttcccag acggtcggcg cggagcttca cctggagcgg cgattcttca
     1261 ccggagacgt acag
//
LOCUS       DASUCJ010001098         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_225556, whole genome shotgun sequence.
ACCESSION   DASUCJ010001098 DASUCJ010000000
VERSION     DASUCJ010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225556"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="selA"
                     /locus_tag="VFU90_12905"
     CDS             complement(<1..713)
                     /gene="selA"
                     /locus_tag="VFU90_12905"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5480735.1"
                     /translation="MDERRRLPAVHRLLAEPAMAEYEAALGRETVKRAVERELDRARA
                     STPPPGYDAVLEAVLTRLRDARLQEMIPAINATGVLAHTNLGRVPLAPGALAAVERIA
                     HGYSNLEYDLAAGERGSRYDRVAGTICELTGAPAALVTNNCAAALLLILDTFAKGREA
                     VVARGQLLEIGGGFRIPEILERSGATLREVGTANRTYVRDFERALSPRTALLLRVHPS
                     NYRIEGFTHEVDARELAALG"
     gene            complement(715..1041)
                     /gene="trxA"
                     /locus_tag="VFU90_12910"
     CDS             complement(715..1041)
                     /gene="trxA"
                     /locus_tag="VFU90_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5480736.1"
                     /translation="MSALADVSQTDFDSEVLQNSQPVLVDFWAPWCGPCKMLGPVVEK
                     VAAAHAGKAKFVKLNTDDNPSLAGKYQVSGIPCLILFKGGQPVDRIVGYVPENVITSM
                     LGKHVN"
     gene            complement(1103..>2050)
                     /locus_tag="VFU90_12915"
     CDS             complement(1103..>2050)
                     /locus_tag="VFU90_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU5480737.1"
                     /translation="GYIKRVSVDTFKTQNRGGRGVIGISNLKREDVVRNFFITKTHDH
                     VLFFTNKGRAYRLRGFEIPDTTRQARGTALVNLLTLPPGEEVTAVFPVHNFGGDRYMI
                     MVTQNGVIKKTKLEEFANVRRNGLNAINLDDADELLAVDLSDGSHDIILGSVRGMAVH
                     FNETNVRPMGRNARGVKAMTLENGDTIVAMDVIADQRREVLLVTSLGFGKRTPIEDYR
                     HTARGGKGVKAFARQRDDIGQVVDQILVEPEDQILMITSGNQVIRLKVGEIRKTGRDA
                     KGVRLQRLGEGEEVIAITNLGKQAKQITDITGEPQQSEL"
BASE COUNT          314 a          670 c          659 g          407 t
ORIGIN      
        1 ccgagcgccg ccagctcgcg agcgtcgacc tcgtgcgtaa atccttcgat ccggtagttc
       61 gacggatgca cgcgcagcag cagggcggtg cgcggcgaaa gcgcgcgctc gaagtcgcgc
      121 acatacgtcc ggttggccgt accgacctcg cgcagcgtcg cgccgctgcg ttcgagaatt
      181 tcgggaatgc ggaaaccgcc gccgatttcg agcagctgtc cgcgcgcgac gaccgcttcg
      241 cgtcctttgg cgaacgtgtc gaggatcagc agcagcgcgg ccgcacagtt gttggttacc
      301 agcgccgccg gcgcaccggt cagctcgcaa atcgtcccgg caacgcgatc gtatcgcgaa
      361 ccgcgttcgc cggcggccag atcgtattcg agattggagt agccgtgcgc gattcgctcg
      421 acggcggcga gcgctccggg cgcgagcggg acgcggccca agttggtgtg cgcgaggacg
      481 ccggtcgcat tgatcgccgg gatcatctct tgcagtcgcg cgtcgcgcaa gcgggtgagg
      541 acggcctcca gcaccgcgtc gtagcccggc ggcggggtgg aagcgcgcgc gcggtcgagc
      601 tctcgctcca ccgcgcgttt gaccgtttct cgaccgagcg ccgcctcgta ctccgccatc
      661 gccggctccg ccagcaggcg gtgtacggcc ggcaggcgcc ggcgctcgtc catattagtt
      721 cacgtgtttg ccgagcatcg acgtgatcac gttctcggga acgtatccga cgatgcgatc
      781 caccggctga ccgcccttga agagaatcag acatggtatt ccggacacct gatacttgcc
      841 ggccaagctc ggattgtcgt cggtgttcag cttcacgaac ttggccttgc cggcgtgcgc
      901 ggcggccact ttctcgacga ccggtccgag cattttgcac ggtccgcacc acggggccca
      961 aaagtccacc agcacgggtt ggctgttttg caagacttcg ctgtcgaagt ccgtctgcga
     1021 gacgtctgcg agtgcgctca tatcggtcgt ttcgattcct ccggttgcta aagactcccc
     1081 cgcgggttgc ggggttgcac ccctacagct cggactgctg gggctcgccc gtgatgtcgg
     1141 taatctgctt cgcctgtttg ccgagattgg tgatcgcgat gacctcttct ccctcgccga
     1201 gccgctgcag ccgcacgccc ttggcgtcgc gtccggtttt gcgaatctcg ccaaccttca
     1261 gacgaatgac ctggttgccc gaggtgatca tcaagatctg gtcctccggc tcgaccaaaa
     1321 tctggtcgac tacctgcccg atgtcgtcgc gctggcgggc gaaagccttg acgcccttcc
     1381 cgccgcgcgc ggtgtgccgg tagtcctcga tcggcgttcg cttcccgaag cccagcgacg
     1441 tcaccagcaa aacttcgcgc cgctggtcgg cgatgacgtc catcgccacg atcgtgtcgc
     1501 cgttctccag cgtcatcgcc ttcacgccgc gggcgttgcg ccccatcggg cgcacgttcg
     1561 tctcgttgaa atggaccgcc atgccgcgca ccgagccgag gatgatgtcg tgcgagccgt
     1621 ccgagaggtc gaccgccagc agctcgtcgg cgtcgtcgag attgatcgcg ttgagaccgt
     1681 tgcggcgcac gttggcgaac tcttcgagtt tcgtcttctt gatcacgccg ttctgcgtga
     1741 ccatgatcat gtagcgatcg ccgccgaagt tgtgcacggg gaataccgcg gtgacttctt
     1801 cgcccggcgg cagcgtaagc agattgacca gcgcggttcc gcgcgcttgg cgcgtggtgt
     1861 cgggaatctc gaagccgcgc agacggtacg cgcgtccttt gttcgtgaag aacaggacgt
     1921 ggtcgtgcgt cttcgtgatg aagaaattgc gcacgacgtc ttcgcgcttg aggttcgaga
     1981 tgccgatgac gccgcggccg ccgcggttct gcgtcttgaa cgtatcgacc gagacgcgct
     2041 tgatgtagcc
//
LOCUS       DASUCJ010001099         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_485401, whole genome shotgun sequence.
ACCESSION   DASUCJ010001099 DASUCJ010000000
VERSION     DASUCJ010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485401"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFU90_12920"
     CDS             complement(<1..291)
                     /locus_tag="VFU90_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480738.1"
                     /translation="MGVSAPAAAKTGGLSTVVDTIAAPADAFERLRTMPTWGWALIVA
                     IVLMVIGTVLQLPAQHHAATSQVQHMVDGPLFAKATAEQQQNAVANAGKTNVF"
     gene            complement(377..649)
                     /locus_tag="VFU90_12925"
     CDS             complement(377..649)
                     /locus_tag="VFU90_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480739.1"
                     /translation="MALRYPTVVALTLALAASLPAAASPRAHRRTVAHPARRPVAADE
                     IYSKQALRRAKIVIELKLLEFRRERLERVRKAIMKNEPIEELLKTP"
     gene            685..>1087
                     /locus_tag="VFU90_12930"
     CDS             685..>1087
                     /locus_tag="VFU90_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5480740.1"
                     /translation="MLKPGSPLIVPDPPAEPYVRIIPLGGCGEIGRNMTLIETNDDMV
                     AVDCGLMFPDQEMYGVDIVINDFTYVRERREKFRAVLVTHGHEDHIGGIPYLLREFNV
                     PVVGTPLTNALIKAKSRDHKMMGEAEFVTVQP"
BASE COUNT          202 a          349 c          339 g          197 t
ORIGIN      
        1 gaacacgttc gtcttaccgg cgttggccac ggcgttctgc tgttgctccg ccgtcgcttt
       61 tgcgaaaagt ggaccgtcga ccatgtgctg cacttgagac gtcgctgcgt gatgctgcgc
      121 gggcaattgc agtaccgttc cgatcaccat caagacgatc gcaacgatga gcgcccatcc
      181 ccacgtcggc atcgtgcgca aacgttcgaa cgcatcggcg ggagcggcaa tggtgtccac
      241 aacagtggaa aggccgcccg tctttgcggc ggctggggcg gaaacaccca tgaaggcctc
      301 ctcggaaaac gacaattgcc gcagggacat acggttgcgg caaatcctgg ttcaagcgta
      361 cctgcgagca agcctcttac ggcgtcttca gcagttcctc gatcggctcg ttcttcataa
      421 tcgccttgcg cacgcgctct agtcgctcgc gccggaattc cagcagtttg agttcgatga
      481 cgatcttcgc ccggcgaagc gcctgctttg aatagatctc atcggccgcg acgggccgcc
      541 gcgcgggatg cgctaccgtg cgacggtgcg ctcgcgggct cgccgcggcc ggcagggagg
      601 cggctagagc caaggtcagc gcgacgacgg tggggtaacg gagtgccatg cgtttccatc
      661 actcccagca ggcggttcgg ctagatgctc aagcccggta gcccgctcat cgtcccagac
      721 ccccccgccg agccgtacgt ccgcatcatt ccgctgggcg gatgcggcga gatcgggcgc
      781 aacatgacgc tcatcgagac gaacgacgac atggtcgccg tcgactgcgg gctcatgttc
      841 cccgaccaag agatgtacgg cgtcgacatc gtcatcaacg atttcacgta cgtccgcgag
      901 cgccgcgaga agttccgcgc cgtcttggtc acgcacgggc acgaagacca catcggcggc
      961 attccgtatc tgctgcgcga gttcaacgtg cccgtcgtcg ggacgccgct gaccaatgcg
     1021 ctcatcaaag cgaagtcgcg cgaccacaag atgatgggcg aggccgagtt cgtgacggtg
     1081 cagccgg
//
LOCUS       DASUCJ010001100         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_851947, whole genome shotgun sequence.
ACCESSION   DASUCJ010001100 DASUCJ010000000
VERSION     DASUCJ010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851947"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFU90_12935"
     CDS             <1..1131
                     /locus_tag="VFU90_12935"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU5480741.1"
                     /translation="TDARARAGGGSLAAEGVASLPSIRNLRDATLALNAEAAGARIDA
                     PSYFKGRIDADVHVVRAAGARPLVSGSVALSSARIPLAALYDPRAANGQTPHLPDAAF
                     DLTVAANRDVRVVSPNVDVGATGSVHVMGTTASPRLNGRFDSSGGTVSFLRDFRVRHA
                     SVAFDPADGAIPTVDAVATTYIDDPGTDVRLHVTGPATSLHLGLASNPAYDREQILGL
                     LVNAQSLGAVRGVTARGTPVSATAEAGQLAGDQLDRVFTRNLLEPLSLALGSSLGLQN
                     LQITSDVQNGLGIDAVKAFGKSVNFVFAESFNEERRTSWSLQARPSSTTRLDLTAYTA
                     QNVNAFVLQPPPAQNEPDAATATIPRDSGTNGVNVEFRSTFP"
     gene            1128..2192
                     /locus_tag="VFU90_12940"
     CDS             1128..2192
                     /locus_tag="VFU90_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480742.1"
                     /translation="MRTLGIVLASLLAACAAPPVAPAPHGPPARVDLSRALAQHPFAS
                     VLAQYDRDIAALQLSRSTTAFGDFDRRLDAGATGVAREVSRASAVLQNLQPRTSAAPP
                     SRDGAAANPLGSTTLATYQTASAERIDHALQLRGQQLREDEANVALAFERAHAGTRLQ
                     LVVKLNDLHLDAPTRKAYRSRLIALDAAEAGLVNARRRRDASTLTAYAARLRAQAATD
                     AATLADELDRHRRAAASIPSSEGTRLPADLVRDDRAHAAAALAAASSDATERAKELRA
                     SNDAAQTGLSAEIATLTRERDRLRDEMIADVETRAQAIARTRGLGRLYAGAAPANARD
                     VTGAVVRELQNDDRLTAAAP"
     gene            complement(2198..2473)
                     /locus_tag="VFU90_12945"
     CDS             complement(2198..2473)
                     /locus_tag="VFU90_12945"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEU5480743.1"
                     /translation="MPAKKWSARVTRESNALDLEPDVFAHDDPAEIARSLKRSAERSK
                     RRKSSSYRSAMSMLTFYINRAGKNLPKRRLQILERAKDELRKAFGRE"
BASE COUNT          488 a          985 c          935 g          413 t
ORIGIN      
        1 acggacgcac gagcccgagc cgggggtggg agcttggccg cggagggcgt cgcctcgctg
       61 ccctcgatcc gcaacttgcg cgacgcgacg ctcgccctga acgccgaggc cgccggcgcg
      121 cgcatcgacg cgccgagcta cttcaaagga cggatcgacg cggacgtgca cgtcgtccgc
      181 gcggcgggcg cgcgtccgct cgtgtccgga agcgtcgcgc tgagtagtgc gcgcattccg
      241 ctcgcggcgc tgtacgaccc cagggccgcc aatggccaaa cgccgcacct tcccgacgcg
      301 gctttcgacc tgacggttgc ggcgaatcgc gacgtccgcg tcgtcagccc gaacgtggac
      361 gtcggcgcga ccggctcggt gcacgtgatg ggaacgacgg cgtcgccgcg gttgaacgga
      421 aggttcgact cgtccggcgg aaccgtgagc ttcctgcgcg attttcgcgt ccgccacgcc
      481 agcgtcgcgt tcgatccggc cgacggcgcg atcccgaccg tcgacgccgt agcgacgacg
      541 tacatcgacg accccggtac cgacgtgcgg ctgcacgtta ccggcccagc caccagcctg
      601 cacctcgggc tcgcatcgaa tccggcatac gaccgcgagc aaattctcgg attgttggtg
      661 aacgcgcaat cgctcggcgc ggtgcgcggc gtcaccgcgc gcggcacccc cgtttcggca
      721 acggccgaag ccgggcagct ggccggcgat cagctggatc gggtcttcac gcgcaacctg
      781 ctcgagccgt tgtcgctcgc gctcggcagc agcctgggtt tgcaaaacct gcagatcacc
      841 agcgacgtgc agaacgggct gggtatcgat gccgtgaagg cgttcggaaa gtcggtgaac
      901 ttcgtcttcg cggaatcgtt caacgaagaa cgccgcacgt cgtggagcct gcaggctcgg
      961 ccgagcagca cgacccgtct cgacttgacg gcctacaccg cacaaaacgt caacgcgttc
     1021 gttctgcagc cgccgccggc gcagaacgag cccgacgcgg cgaccgcaac gattccgcgc
     1081 gactcgggaa cgaacggcgt caacgtggag ttcaggagta cgtttccgtg agaacgctcg
     1141 gcatcgtgct cgcgagcctg ctggccgcct gcgcggcgcc gcccgttgcg ccggcaccgc
     1201 acgggccgcc ggcacgggtc gatctgtcgc gcgcgcttgc gcagcatccg ttcgcctcag
     1261 tcctcgcgca gtacgaccga gacatcgcgg cgctgcaact cagccgctcg acgacggcgt
     1321 tcggcgactt cgaccgccgg cttgacgccg gtgcgaccgg cgtcgcccgc gaggttagcc
     1381 gcgcatcggc cgttctgcaa aacctccagc cgcgcacgtc ggccgcgcct ccgtcacgcg
     1441 acggtgccgc tgcgaatccg ttggggagta cgacgctcgc gacgtaccaa acggcgtcgg
     1501 ccgaacggat cgaccacgcg ctccaactgc gcgggcaaca gctgcgcgag gacgaagcca
     1561 acgtcgcgct tgccttcgag cgcgcgcatg cgggcacgcg cctgcagctc gtggttaagc
     1621 tgaacgatct tcacctggac gcgccgacgc gcaaagcgta ccggagccgc ttgatcgcac
     1681 tcgacgccgc cgaggcgggg ctcgtgaacg cgcgccgccg gcgggatgca tcgacgttga
     1741 cggcctacgc cgcgcgcttg cgggcgcaag ctgcgaccga tgccgcgacc ctggcggacg
     1801 agctggaccg tcaccgccgc gcggccgctt cgattccctc atccgaaggc acccgtctgc
     1861 ccgcagacct cgtccgcgac gaccgcgcgc acgccgccgc ggcgcttgcc gccgcgagca
     1921 gcgatgcaac cgaacgcgcg aaagaactgc gcgcctccaa cgacgccgcg cagaccgggc
     1981 tttcggcgga aatcgcaacg ctgacgcgcg agcgcgatcg actgcgcgac gaaatgatcg
     2041 cggacgtcga aacgcgcgcg caagcgatcg cacgaacgcg cgggctggga cggctctatg
     2101 ccggcgcggc gccggcaaat gcgcgggacg tcaccggggc ggtggttcgg gagttgcaaa
     2161 acgacgaccg gttgacggcc gccgcgccct agccggttta ttcccggccg aaagccttgc
     2221 gcagctcgtc cttcgcgcgc tcgaggattt ggagacggcg cttcggcaga ttcttgcccg
     2281 cgcgattgat gtaaaacgtc agcatcgaca tagcggagcg gtaggaactg gatttacgcc
     2341 gcttgctgcg ctcggcggaa cgcttcaacg accgcgcgat ctccgccgga tcgtcatggg
     2401 caaacacgtc cggctccaga tcgagcgcgt tgctctcgcg cgtaacgcgc gcggaccact
     2461 tcttggcagg cacctagctt cggtcgtctt tcattcgatc gcctcgctcc tcacggttgg
     2521 actttaccca accggtacgg acccttcgga actgggcgtt tggccctttc ccgccgaagg
     2581 tacaacgcta gtagtcagca gctgcgttgg gaggacgtat ggcgcgaaaa gctacccgaa
     2641 agacggctcg cacgagcgct cgtaagaaga ccactcgcaa gaaatctgcc ggaacgaccc
     2701 gcaagggcgc ggcgcgcaag aaggccgacg gccgcaagaa gacggctcgt aagacgacca
     2761 gtcgtaagaa gacgactcgc aaagctgcgg cctctcgtaa gaaggggacg cgcaaatatt
     2821 c
//
LOCUS       DASUCJ010001101         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_592649, whole genome shotgun sequence.
ACCESSION   DASUCJ010001101 DASUCJ010000000
VERSION     DASUCJ010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592649"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFU90_12950"
     CDS             complement(<1..887)
                     /locus_tag="VFU90_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5480744.1"
                     /translation="MPTSRKTSGARPGSLATYHKKRDFALTPEPAGKKAAAGKRLRFV
                     VQMHRATRLHYDFRLEADGVMASWAVPKGPTLAPLDRRLAMHVEDHPLDYRDFEGNIP
                     EGQYGAGSVIVWDKGTYALAEGEDPAAEIANGKIKFVLHGKKLRGEFTLVRIKGREGE
                     HGDPWLLIKDRDEHADPKYDPADQPESVKSGKTLADIAANPRSRIWNSKPKSRQATAP
                     KLPSRVKRDPLPHLKSVMLATLVDRPFDDDEWLFEIKWDGYRVICTIDERGKLALTSR
                     NGLDLLARFPSLAALADAF"
     gene            978..1256
                     /locus_tag="VFU90_12955"
     CDS             978..1256
                     /locus_tag="VFU90_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480745.1"
                     /translation="MWSLAGLALSAAIATAAWLRSRARGGYYDRDVYAMSGRAHRAYA
                     LVSLAFAAFFAVALALRREAAGVGGLALYAVIAAFYAASFLRGADDYE"
     gene            1249..3009
                     /locus_tag="VFU90_12960"
     CDS             1249..3009
                     /locus_tag="VFU90_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEU5480746.1"
                     /translation="MNEFRFSPRPNRAAEIGWMPWEPSSFARARSEDKPILLAISAVW
                     CHWCHVMDETTYSDPAVIEAINARFVPIRVDNDRRPDVNARYNMGGWPTTAFLAPDGA
                     LLTGTTYLPAEPMRRALDEIARFYAEKKDEIATHAPPARRADYRPAPAADLREGMIVR
                     LVEQLTENFDDEYGGFGDAPKFPQPEMLEFLLARWRATGEPRLHAMVAKTMLGMSRGG
                     TYDHVEGGFFRYSTTRDWSVPHFEKMAEDHAGLLRVLAALVVFAPAAEFRATLASAIA
                     YVRTTLRDPASSLFGGSQDADEAYFALPLEERRKRAGPFVDRTSYTNWTCALAGAWCA
                     ASLALDDDGLLREACETLDAVHERLLDPGGLAYHFVAPGSAPQVRGLLTDQAAYLRAL
                     LDAHEASGEARFLERAGALAARILARFQAGDGGFYDRIPIEAELGRLDVADRPIVENG
                     LIAEGLLRLGALTGDAAYREPAERTLQLFAHSYAGAGPFAAAYGRALQRYLAPETNVR
                     LVGTPKSTGALREAARRLTSPAVTVRTVDPVGAAALDLPPEPVPAAYACSGSACGAPA
                     RDPAGLRDAYERLAGGPRPA"
BASE COUNT          445 a         1032 c         1038 g          541 t
ORIGIN      
        1 ttgaaggcat cggcgagcgc ggccagcgat ggaaagcgcg ccagcagatc gaggccgttg
       61 cgcgacgtca gcgcgagctt tccccgttcg tcgatcgtac agatcacgcg gtagccgtcc
      121 cacttgatct cgaacagcca ctcgtcgtcg tcgaacggac gatcgacgag cgtcgccagc
      181 attaccgatt tcagatgcgg cagcggatcg cgcttgacgc gcgacggtaa tttcggtgcg
      241 gtcgcctgac gcgatttcgg tttcgagttc cagattctgg atcgcggatt cgcggcgatg
      301 tcggccagcg tcttgcccga tttcaccgac tccggctggt ctgcgggatc gtatttcgga
      361 tcggcgtgct cgtcgcgatc tttgatcagc agccacggat ctccgtgctc gccctcgcgg
      421 cccttgatcc gcaccagcgt aaactcgccg cgcagcttct tgccgtgcaa aacgaatttg
      481 atcttgccgt tggcgatttc ggccgccgga tcttcgccct cggccagcgc gtaggtgcct
      541 ttatcccaaa cgatcacgct gccggcgccg tactgtcctt cgggaatatt gccctcgaaa
      601 tcgcggtagt cgagcggatg gtcctcgacg tgcatcgcca gccggcgatc cagcggcgcg
      661 agcgtcggtc ccttgggcac cgcccaggat gccatcacgc cgtcggcttc gagacggaag
      721 tcgtaatgca ggcgagtcgc ccgatgcatc tgcacgacga agcgcagacg tttgcccgcc
      781 gcggcttttt tgccggccgg ctcgggggtg agcgcgaagt cgcgcttctt atgatacgta
      841 gcgagcgagc caggtcgcgc tccgctcgtt ttgcgcgagg tcggcatgcc cccgtgttcg
      901 acggatggcg gcggctccat ccgcggcggg cggttcgccg gaaacctttt ctgcaagcaa
      961 tcgcgaagtt tgcccgcatg tggagtctcg ccgggctcgc gctctccgcc gcgatcgcga
     1021 ccgccgcctg gttgcgcagc cgcgcgcgcg gcggttatta cgatcgcgac gtttacgcga
     1081 tgagcgggcg agcccaccgc gcgtacgcac tcgtcagcct ggcgttcgcc gcctttttcg
     1141 cggtcgcact cgcgctgcgg cgcgaggcgg caggcgtcgg cgggctcgcg ctgtatgcgg
     1201 tgattgcggc gttttatgcc gcgtccttcc tgcgaggcgc cgacgactat gaatgagttt
     1261 cgcttctccc cgcgtcccaa ccgcgccgcc gaaatcggct ggatgccgtg ggagccgtcg
     1321 tcgtttgcac gcgcgcggtc ggaagacaag ccgatcctgc tcgcgatttc ggcggtatgg
     1381 tgccattggt gtcacgtcat ggacgagacg acctactccg atccggcggt catcgaagcg
     1441 atcaacgcgc gcttcgtccc gattcgcgtg gataacgacc gccggcccga cgtcaacgcc
     1501 cgctacaaca tggggggatg gccgacgacg gccttcctcg cacccgacgg agccttgctt
     1561 accggcacca cctatcttcc ggccgagccg atgcggcgcg cgctcgacga aatcgcgcgg
     1621 ttctacgccg aaaagaagga cgagatcgcg acgcacgcac cgccggcgcg gcgcgcggac
     1681 tatcggccgg cgccggcggc cgatttgcgc gagggcatga tcgtccggtt ggtggagcag
     1741 ctcaccgaaa acttcgacga cgaatacggc ggcttcggcg acgccccgaa atttccgcaa
     1801 ccggaaatgc tcgagttctt gctggcccgg tggcgcgcga cgggcgaacc gcgactgcac
     1861 gcgatggtgg ccaagacgat gctcggaatg tcgcgcggcg gaacttacga ccacgtcgaa
     1921 ggcggtttct tccggtattc gacgacgcgc gactggtcgg tgccgcattt cgaaaagatg
     1981 gccgaagatc acgccggtct gctgcgggtg ctggccgcgc tggtcgtctt cgcgccggcg
     2041 gcagagtttc gcgcgaccct cgcttcggcg atcgcgtacg tccgtacgac gctgcgcgat
     2101 ccggcgtcgt cgctgttcgg cggcagccaa gacgccgacg aagcgtactt tgcgctgccg
     2161 ctcgaggagc gccgcaaacg cgccggcccg ttcgtcgacc ggacgtcgta cacgaactgg
     2221 acgtgcgcgc tggcaggcgc gtggtgcgcg gcgagcttgg cgctggacga cgacggattg
     2281 ctgcgcgaag cgtgcgagac gctcgacgcg gtgcacgagc ggcttttgga tcccggcggg
     2341 ctcgcgtacc acttcgtcgc gcccggcagc gcaccgcagg tgcgcggttt gctgaccgat
     2401 caggccgcct acttgcgcgc gctgctcgac gcgcacgaag cgtcgggcga agcacgcttt
     2461 ctcgagcgcg cgggagcgct cgcggcccgc atcttagcgc gcttccaggc cggcgacggc
     2521 ggtttctacg accgaattcc gatcgaagcc gaactcggcc ggctcgacgt tgcggatcgt
     2581 ccgatcgtcg agaacggttt gatcgcggaa ggtttgctgc ggctcggcgc gctgaccggc
     2641 gacgccgcct accgggagcc ggccgagcgt acgctgcagc tgttcgcgca ctcctacgcg
     2701 ggggcgggtc cgtttgcggc cgcttacggc cgcgcgctgc aacgctatct ggcccccgag
     2761 acgaacgtcc ggctcgtcgg cacgccgaaa tcgaccggcg cgctgcgcga agcggcgcgc
     2821 cgcttgacct cgccggccgt cacggtgcga accgtcgatc cggtcggcgc ggccgcgctg
     2881 gacctgccgc cggaacccgt ccccgcggcc tacgcgtgct cgggcagcgc gtgcggcgcg
     2941 ccggcccgcg acccggccgg gctgcgggac gcctacgaac gcctggcggg cggccctcgg
     3001 ccggcttgaa actccggccg tccttcgccg tatggatggg tggaggacac catctt
//
LOCUS       DASUCJ010001102         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_561998, whole genome shotgun sequence.
ACCESSION   DASUCJ010001102 DASUCJ010000000
VERSION     DASUCJ010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561998"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFU90_12965"
     CDS             <1..810
                     /locus_tag="VFU90_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5480747.1"
                     /translation="RFLPADERGVLVLDDLDRLLDGCKLLAVGHVSNTLGTIAPLDVL
                     IPRAHAAGAVVVVDGAQGAPHLPVDVKALDADFYAFSGHKMLGPTGIGVLYGKRALLE
                     AMPPFGAGGDMIHKVEYERSTFAEPPRRFEPGTSNIADAIAFGVAVDYLERVGMDWVR
                     KHERELTGYALERLSEFEGRGLEIYGPRDPAAISGVISFNFADIHAHDLASILDTEGV
                     CVRAGHHCTMPLMEKMGWSSTARASFYLYNSRDDVDVLASALDKAAKVFKL"
     gene            811..1206
                     /locus_tag="VFU90_12970"
     CDS             811..1206
                     /locus_tag="VFU90_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5480748.1"
                     /translation="MDDFYRDYILDHYRSPRNFGHLEHPDAAAEDINPLCGDAIRMEL
                     SVDGNGTVRDVRFSGKGCAISQASASMLTESVKGLPLEDVAKLPKDVVLENVGIGISP
                     TRMKCAMLGLRVLKSAAIGELAGWPDEEA"
     gene            1260..1589
                     /locus_tag="VFU90_12975"
     CDS             1260..1589
                     /locus_tag="VFU90_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEU5480749.1"
                     /translation="MQHRQITPEQWDALAAEPDFRHLVTARRRFVIPATIFFIAYYLA
                     LPISVGFAPQVMSRPVVGPLTLAYCFALSQFAMAWILLALYLARARSFDLQAARIRHR
                     ETREIKA"
     gene            1586..>2215
                     /locus_tag="VFU90_12980"
     CDS             1586..>2215
                     /locus_tag="VFU90_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480750.1"
                     /translation="MSSQPSLLMFGVFVGITLIVTYWASGQSTTSRGFYTAHRRIGGV
                     QNGWAIAGDYMSAASFLGITGLVAFYGFDGFMYSVGWLVAYLAVLFLVAEQLRNTGKY
                     TLADLISFRLRGRGVRAIAAVSTLAITLFYMIAQMVGSGLIVNLLLPRFPDFVAIGVV
                     GVLMLVYVLFGGMLATTWVQIIKAALLLVTAVALSLLVMAHFGFSFAHFL"
BASE COUNT          353 a          729 c          703 g          430 t
ORIGIN      
        1 cgctttctgc ccgccgacga gcgcggggtt ctcgtcctcg acgatctcga ccggctgctc
       61 gacggctgca agctgctcgc ggtcggacac gtcagcaaca cgctcgggac gatcgctccg
      121 ctcgacgttc tcatcccgcg cgcgcacgcg gcgggtgccg tcgtcgtcgt ggacggggcg
      181 cagggggcac cgcatctgcc cgtcgacgtc aaggcgctgg acgccgattt ctacgcgttt
      241 agcggccaca agatgctcgg acccaccgga atcggcgtgc tgtacgggaa gcgggcgctg
      301 ttggaagcga tgccgccgtt cggcgccggc ggcgacatga ttcacaaagt cgaatacgaa
      361 cgctcgacct ttgccgagcc gccgcgccgg ttcgagcccg gcacgagcaa catcgccgac
      421 gcgatcgcct tcggggtggc ggtcgactat ctcgaacgcg tggggatgga ctgggtccgc
      481 aaacacgagc gcgaactcac cggctacgca ctcgaacgtc tgagtgagtt cgaaggccgc
      541 ggcctcgaaa tctacggtcc gcgggatccg gcggcgatct cgggcgtgat ctcgttcaac
      601 ttcgccgaca ttcacgccca cgatctcgcc tcgatcctcg acaccgaagg cgtctgcgtg
      661 cgcgcggggc atcattgcac gatgccgttg atggaaaaaa tgggctggtc ctcgaccgcc
      721 cgcgcgtcgt tctatctcta caactcgcgc gacgacgtcg acgtcctggc gagcgcgctc
      781 gacaaagccg ccaaagtctt caaactctag atggacgact tttaccgcga ttatattctc
      841 gaccactatc gcagcccgcg caatttcggt cacctcgagc atccggatgc ggccgccgaa
      901 gacatcaatc cgctgtgcgg ggacgcgatc cgcatggaac tgagcgtcga cggcaacgga
      961 accgtacgcg acgtgcgatt ttccggcaag ggctgcgcga tcagccaggc ttcggcttcg
     1021 atgctgaccg agtcggtcaa aggcttgccg ctcgaggacg tcgcgaagct ccctaaggac
     1081 gtcgtcctgg agaacgtcgg catcggcatc agcccgacgc gcatgaagtg cgcgatgctc
     1141 gggttgcgcg tgctcaagag cgccgccatc ggcgagctcg cgggctggcc cgacgaagag
     1201 gcgtagggaa accgggcggc tctagaggta gccgcgccct ggcagtaaac ttcgaaggcg
     1261 tgcagcaccg tcagatcacg ccggaacagt gggacgcgct ggccgccgag ccggattttc
     1321 gtcatctggt caccgcccgg cggcgctttg tgatcccggc gacgatcttt ttcattgcat
     1381 attacctcgc actcccaatc tcggtagggt tcgcaccgca ggtgatgagc cggccggtcg
     1441 tcggcccgct cacgctggcg tactgctttg ccctctcgca gttcgcgatg gcgtggatcc
     1501 tgctcgcgct ctacctcgcg cgcgcccgca gcttcgacct tcaggccgcg cgcatccggc
     1561 atcgcgaaac gcgcgagatc aaagcatgag ctcgcagccg tcgctgctca tgttcggcgt
     1621 cttcgtcggc atcacgctga tcgttaccta ttgggcttcc ggccagagca cgaccagccg
     1681 cggattctat accgcgcacc gccggatcgg cggcgtgcag aacggctggg cgatcgccgg
     1741 cgactacatg tcggccgcgt cgttcctcgg tatcacgggg ctcgtggcat tctacggatt
     1801 cgacggtttc atgtattcgg tgggttggct ggtcgcctat ctcgccgttt tgtttctcgt
     1861 cgccgaacag ctgcgcaaca ccggaaagta cacgctcgcc gatctcatct cgttccggct
     1921 gcgcggccgc ggggtgcgcg cgatcgcagc cgtttcgacc ctggcgatca cgttgttcta
     1981 catgatcgcc caaatggtcg gcagcggcct gatcgtcaac ctgctgctgc cgcgctttcc
     2041 cgacttcgtg gcgatcggcg tggtcggcgt gctgatgctc gtctacgtgc tgttcggcgg
     2101 gatgctcgcg accacgtggg tgcaaatcat caaggccgcg ctgctgctgg tgaccgcggt
     2161 ggcgctgtcg ctgctcgtga tggcccactt cggcttttcg ttcgcgcact tcttg
//
LOCUS       DASUCJ010001103         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_959067, whole genome shotgun sequence.
ACCESSION   DASUCJ010001103 DASUCJ010000000
VERSION     DASUCJ010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959067"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFU90_12985"
     CDS             <1..252
                     /locus_tag="VFU90_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480751.1"
                     /translation="FAAAALAAIPSVSFAQDATAGAAPSATGGNGDYGYKFEDDPLSA
                     GGFGPSDATIRVRPGPVRTTLIRPRTSFVPEMLKSVENI"
     gene            310..>1270
                     /locus_tag="VFU90_12990"
     CDS             310..>1270
                     /locus_tag="VFU90_12990"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5480752.1"
                     /translation="MDSQGKAKVALTFALYQGDQLVRRDTIAQDIVKVGKDPRSHLRV
                     DDDLASRMHAVIEVASPVDITLIDLGNEPGTLVNGQRVNKCKIRPGDQIQIGSTLIVL
                     ENAEPATAAPPQVTAPGMMPAAPSSVPASPAAANPFAGSNPFAGSAPSPSANPFAGAN
                     PFAGQASPSAPAAPFAAVAAAYAQAPASTPGLGIEPPLEGQSGFTYSMVKSGPDVSPD
                     EVEVAHLSSIEVMILWDSNVLQVWHLTPPRSFYVGEEFGDKNACDYFIPSETLGTTRA
                     PVVVSRGLGATLVILPRSRGYVDIPGQAKVMLADLVAAGRARPS"
BASE COUNT          215 a          418 c          414 g          223 t
ORIGIN      
        1 ttcgcggcgg cggctctggc ggcaatcccg tcggtgtcct ttgcccaaga tgcgaccgcg
       61 ggcgctgctc cgtcagcaac cgggggaaac ggtgactacg ggtacaagtt cgaagacgat
      121 ccgctgagcg cgggcggatt cggaccgtcg gacgcgacga ttcgcgtgcg tcccggaccc
      181 gtgcgcacga cgttgattcg accgcgaact tctttcgtgc cggaaatgct gaagtccgtc
      241 gaaaatatct agtttcactg agacagcgaa cgtcacaccg cgcgaagcac acgcggcatg
      301 gggttattga tggatagcca gggtaaggca aaggttgcac tcacgttcgc gctgtatcag
      361 ggagatcagc tcgtccgtcg cgatacgatt gcgcaagaca tcgtcaaagt cggaaaagac
      421 ccgcgaagcc atttgcgggt ggacgacgac cttgcgtcgc gaatgcacgc ggtcatcgaa
      481 gttgcgtcgc ccgtcgacat cacgttgatc gacctgggca acgagcccgg cacgctcgtc
      541 aacggccagc gcgtcaacaa gtgcaagatt cggccgggcg atcagattca aatcggctcg
      601 acgctcatcg tcctcgaaaa cgcggagccc gcgacagccg ctccgccgca ggtgaccgcg
      661 ccgggcatga tgccggccgc gccttcgtcg gtgccggcat caccggcggc ggccaatccg
      721 ttcgccggtt cgaatccgtt tgccggctcg gctccgagcc cgagcgcgaa cccgtttgcg
      781 ggggcaaacc ccttcgcggg ccaggcgtcg ccctccgccc ctgctgctcc tttcgctgcg
      841 gttgcggctg cctatgcgca ggcgcccgcg tccactccgg ggctcgggat cgagccgccg
      901 ctcgaagggc aatcgggctt cacctactcg atggtcaaga gcggccccga cgtcagcccg
      961 gacgaggtcg aggtcgcgca cctctcgtcg atcgaagtga tgattctgtg ggactcgaac
     1021 gtcctgcagg tgtggcactt gacgccccct cggtcgttct acgtcggcga ggagttcggc
     1081 gacaagaacg cgtgcgacta cttcataccg agcgagacgc tcggcacgac gcgcgcgccc
     1141 gtggtcgtct cgcgcgggct cggtgcgacc ctcgtcattc ttccgcggtc gcgcggctac
     1201 gtggatatcc ccggtcaggc aaaggtgatg ctcgccgacc tcgtcgcggc ggggcgcgcg
     1261 cgtccttcga
//
LOCUS       DASUCJ010001104         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_699228, whole genome shotgun sequence.
ACCESSION   DASUCJ010001104 DASUCJ010000000
VERSION     DASUCJ010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699228"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFU90_12995"
     CDS             <1..981
                     /locus_tag="VFU90_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU5480753.1"
                     /translation="QNAWMYFGGADTISKTVAAATNNKFQIQTFAGGEIVPAFGVVDA
                     VQNATVQCAHTAPYYFFGKDPTFALGCALPFGMNARQQNAWMYFGGGLELMREFYKDY
                     NIINFPAGNTGAQMGGFFRKPIKSVADFKGLKMRIGGTGGLPLQKLGLVPQQIPGGDI
                     YPALEKGTIDAAEWVGPYDDEKLGLYKVARYYYYPGWWEGGPELDLFVNIKAWNDLPK
                     EYQSVLESACAEANVEMVAKYDALNPPALKRLIAGGVKLEPFSNEIMAACYKATTEVY
                     DGIAEKNAKFKKVYTSWKQFRNDEVSWFQVAEDRFNNFMIAAARMSQGRK"
BASE COUNT          211 a          337 c          371 g          180 t
ORIGIN      
        1 cagaacgcgt ggatgtactt cggcggcgcc gacacgatca gcaagacggt cgccgcggcg
       61 acgaacaaca agttccagat ccagacgttt gccggcggcg agatcgttcc cgcgttcggc
      121 gtcgtcgacg cggtccagaa cgcgaccgtg cagtgcgcgc acaccgcgcc gtactatttc
      181 ttcggcaagg atccgacgtt cgcgctggga tgcgcgctcc cgttcggcat gaacgcgcgt
      241 cagcagaacg cgtggatgta cttcggcggc gggctcgagc tgatgcgcga gttctacaag
      301 gactacaaca tcatcaattt cccggcgggc aacaccgggg cgcagatggg cggcttcttc
      361 cgcaagccga tcaagtcggt cgccgacttc aagggcctca agatgcgcat cggtggcacc
      421 ggcggattgc cgctgcagaa actcgggctc gtcccgcagc agattcccgg cggcgacatc
      481 tacccggcgc tggagaaggg cacgatcgac gcggcggaat gggtcggccc gtacgacgac
      541 gagaagctcg ggctctacaa ggtcgccagg tactactact acccgggctg gtgggaaggc
      601 gggcctgagc tcgatctctt cgtcaacatc aaggcgtgga acgacctgcc gaaggaatac
      661 cagtcggtgc tggaatcggc ctgcgccgag gcgaacgtcg agatggtcgc caagtacgac
      721 gcgctgaatc cgcccgcgtt gaagcggctg atcgccgggg gcgtgaagct cgagccgttc
      781 tcgaacgaga tcatggcggc gtgctacaag gcgacgaccg aggtctacga cgggatcgcc
      841 gaaaagaacg cgaagttcaa gaaggtctac acctcgtgga agcagttccg caatgacgag
      901 gtgtcgtggt tccaggtcgc cgaggaccgc ttcaacaact tcatgatcgc tgcggcgcgc
      961 atgtcgcagg gcaggaagta gcggcaggaa gtctcggcaa tctcccggcg cgccgggcgt
     1021 cttgcgcggc cccgcttcgg cggggccgcg tcgtttccgc tgcgtgacgc gttacttgcc
     1081 ggggctcgac ccgaatgcc
//
LOCUS       DASUCJ010001105         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_225717, whole genome shotgun sequence.
ACCESSION   DASUCJ010001105 DASUCJ010000000
VERSION     DASUCJ010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225717"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..>1135
                     /locus_tag="VFU90_13000"
     CDS             203..>1135
                     /locus_tag="VFU90_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480754.1"
                     /translation="MSSTADLTHSPDVRPARAEKVARWAGQADVVIAGYGIAGVCAAI
                     EAARAGAEVLILERTGGWGGAASLSGGFIYLGGGTPLQKALGFHDSAENMEAFLLAAV
                     GPGADRAKVGDYCRGSVEHFNWLVDCGVPFRAAFWSEPGWEPPHDEGLMYSGGENAAP
                     FDTIAVPTPRGHVPQMENKRTGEKGGGFMLMKPLAVAAEKLGVRFEYDTRLRRLIVDD
                     DGRVVGVIATQYGKELAIRATRGVVLATGSFAYNDSMVRAHAPRLIGRPAAAIEEHDG
                     IGIRVAQALGADTAHMDATEVAFLADPQMVVRGIL"
BASE COUNT          200 a          361 c          394 g          180 t
ORIGIN      
        1 tggatcgatt ccggggactt cgccgaactg gaggcgtcgc atcgcgcggg gatgcagatg
       61 atcgtgtccg ccatccggcg acgcgaatac ggcgtcgagt gcgacctcgc gcagaatggc
      121 gaacaactaa cttcctagcc cgatatccca gtagctagaa gacggcattg cgggacgcgc
      181 gacggcgtcg tagcgtcccg gcgtgtcctc tacagccgac ctcacccatt ctcccgacgt
      241 gcgtcccgcg agagccgaga aggtcgcgcg ctgggccggg caagctgacg tcgtcatcgc
      301 aggctatggc atcgccggtg tctgcgccgc gatcgaagcc gcccgcgctg gcgccgaggt
      361 actcatcctc gaacgcacgg gtggctgggg cggtgccgcg tcgctgtccg gaggattcat
      421 ctacctcggc ggcgggacgc cgctgcaaaa ggccctgggc ttccacgact cggcggagaa
      481 catggaggcg tttctgctgg cagcggtggg accgggggcg gaccgcgcga aggtcggcga
      541 ctactgccgc ggcagcgtgg aacatttcaa ttggctcgtc gactgtggcg tacctttccg
      601 ggccgcgttt tggagtgaac cggggtggga gccaccgcac gacgagggac tgatgtactc
      661 cggcggcgaa aacgccgcgc cgttcgatac gatcgccgtc ccgacgcctc gcgggcatgt
      721 gccgcagatg gaaaacaagc gcaccggcga gaagggcggc ggcttcatgc tgatgaagcc
      781 gctggccgtc gccgccgaaa aactcggtgt tcggttcgaa tacgacaccc ggctacggcg
      841 actgatcgtt gacgacgacg gccgagtggt cggcgtgatc gcaacgcaat acggcaaaga
      901 gctcgcgata cgcgccaccc gaggcgtggt cttggccacc ggaagtttcg catacaacga
      961 cagcatggtg cgggcgcatg ccccgcgatt gatcggccgg ccggcggcgg ccatcgaaga
     1021 gcacgacggc atcggcatcc gcgtcgccca ggcccttggc gctgacaccg cccacatgga
     1081 cgccaccgaa gtggcgttct tggcggatcc gcaaatggtg gtccgcggca ttctg
//
LOCUS       DASUCJ010001106         1326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1463747, whole genome shotgun sequence.
ACCESSION   DASUCJ010001106 DASUCJ010000000
VERSION     DASUCJ010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463747"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VFU90_13005"
     CDS             complement(<1..1295)
                     /locus_tag="VFU90_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480755.1"
                     /translation="MRGDSTQPETAAMKKRIAEFRAKQKADSIDLDLRYNWDTPFFIS
                     PHSASTLYIGGNRVLKSTDRGDHIYPISPDLSTKDSVKVRISTKTTGGITNDATGAET
                     YGTITTLAESYLKPGLLYAGTDDGNVWLSPNDGATWENITTRFPGVPPRTYVVRIEPS
                     HFDTLTFYVAFDGHRTNDFTPYLYVTNDGGKTFRSLVNDLPKDGPAFLHVVREDPENR
                     DLLFVGTDVAAYVSIDRGAHWQRFMTGLPTVPVNDLKIQPRDHDLIAATHGRSIWIVN
                     IGPLEQMTDTVLAQSQYFFKPGIAYQYGEAPGPDLNPGQELFEGRSAPYGADFAYRLT
                     NGKRGDTVHIVVTDAKGDTVAAMRGPGGDGLQHVSWNMRGKAPKAKPLSPAAKRDSII
                     SAHKIDHVLDSLAAAKTAPQPVIDRVKKAIVSGGGFGEL"
BASE COUNT          199 a          403 c          468 g          256 t
ORIGIN      
        1 aacagctcgc cgaaccctcc gccgctgacg atcgccttct tgacccggtc gatcaccggc
       61 tgcggcgccg tcttggcggc agccagcgaa tcgagcacgt ggtcgatctt gtgcgcgctg
      121 atgatgctgt cgcgcttggc ggccggcgag agcggcttgg ccttgggtgc tttcccgcgc
      181 atgttccacg acacgtgctg caggccatcg ccgcccgggc cgcgcatggc cgcgaccgtg
      241 tcgcccttgg cgtcggtgac gacgatgtgc acggtgtccc cgcgcttgcc gttcgttagg
      301 cgataggcga agtcggcgcc gtacggcgcg ctgcgtccct cgaacagctc ctggccggga
      361 ttcagatcgg gccccggcgc ctcgccgtac tggtacgcga tgcccggctt gaagaagtac
      421 tgcgactgcg cgagcaccgt atcggtcatc tgctcgagcg gaccgatgtt cacgatccag
      481 atgctgcgcc cgtgcgtcgc cgcgatgagg tcgtgatcgc gcggctggat cttgaggtcg
      541 ttcaccggga cggtcggcag gccggtcatg aagcgctgcc agtgcgcgcc gcgatcgatc
      601 gagacatacg cggcgacgtc ggtgccgacg aacagcaggt cgcggttttc cggatcctcg
      661 cggacgacgt gcagaaacgc ggggccgtcc ttcggcaaat cgttgacgag ggagcggaag
      721 gtcttgccgc cgtcgttcgt cacgtagagg tacggcgtga aatcgttggt gcggtgcccg
      781 tcgaacgcga cgtagaacgt gagggtgtcg aagtgcgagg gctcgatgcg caccacgtac
      841 gtgcgcggcg gaactcccgg gaatcgcgtc gtgatgttct cccaagtggc gccatcgtta
      901 ggcgaaagcc acacgttgcc gtcgtcggtg cccgcgtaca gcaggccggg cttgaggtac
      961 gattcggcga gggtcgtgat ggtgccgtac gtctcggcgc cggtggcgtc gttggtgatg
     1021 ccgcccgtcg tcttggtgct gatgcgaacc ttgacggaat ccttcgtcga gaggtcgggc
     1081 gagatcgggt agatgtgatc gccgcgatcc gtcgatttga gtacgcggtt gccgccgatg
     1141 tacagcgtgc tcgccgagtg cggcgagatg aagaacggcg tgtcccaatt gtagcggaga
     1201 tcgaggtcga tcgaatctgc cttctgcttc gcgcggaact cggcgatgcg cttcttcatg
     1261 gccgccgttt ccggctgcgt ggagtcgccg cgcacgacga ggatcgaatc ttcccacgcg
     1321 agatgc
//
LOCUS       DASUCJ010001107         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_179871, whole genome shotgun sequence.
ACCESSION   DASUCJ010001107 DASUCJ010000000
VERSION     DASUCJ010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179871"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFU90_13010"
     CDS             complement(<1..315)
                     /locus_tag="VFU90_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480756.1"
                     /translation="MNATIRLFILHRHNLFRDLVIHALAADERVRIVGATESQMEAAR
                     WVRDEAASAAIVEVDPGFASGDDVRELFHEWSRQRAFAVVGAGFSQPGIEVYLHKTHS
                     ASA"
     gene            889..>1328
                     /locus_tag="VFU90_13015"
     CDS             889..>1328
                     /locus_tag="VFU90_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5480757.1"
                     /translation="MTIRLYLVCDHLLFRTGLTRLLRREPDVHVVGECPLGLDAADQV
                     RVADADVVLLSVDTPNTASAPAAQRLYRDLMNVPVVLLLMGDDGDDALAALRLGARGV
                     LDKHTDGTRLVNALRQAVVGEITVTPRLATRLVAEYAAIATGRKP"
BASE COUNT          216 a          450 c          428 g          234 t
ORIGIN      
        1 tgcactcgcc gaatgcgtct tgtgcaggta cacctcgata ccgggctggg agaaaccggc
       61 gccgacaacg gcgaatgccc gctgccggct ccattcgtgg aacagctcgc gcacgtcgtc
      121 gccgctcgcg aagccagggt cgacttcgac gatggcggcg ctggccgcct cgtcgcgcac
      181 ccaccgcgcc gcttccatct ggctctccgt ggcgccgacg atgcgcacgc gctcatcggc
      241 cgcgagcgca tggatcacga gatcccggaa caggttgtgg cgatgcagga tgaagaggcg
      301 gatcgtcgcg ttcatctcac aacctgtcac gggtccatcc cgcgtctgcg gcagtccttg
      361 ccggtccatg cagcttcact ccgagcgtac gtggcgcggc cggcccccgc atcgcgggga
      421 tcgccaaccg tcacgtgaca tagtccgcca cgttttctgc gtccgaaacg atgattcgat
      481 cgtgtccggt tggactacgt cacgcggacg tcgggctgtt gagatacggc tacgctacgg
      541 ttcaaaacgc atcgtggcca tacgtcattc ggtgttgacg agagtcgtta cgtcgcgtgc
      601 agacacgtca ataacgcgat tgacaagttg agcgtcggca tgcttcaatc gcggcgtact
      661 tcctccgcga ggcggaagaa ctcgccgcaa atcgtgcagg gaacgcaaac cggggcgctg
      721 tgatcggcca ttggatgggg gctggtcagg cgctgcaagg atgcgctgtt ccgtgcatcg
      781 acctcggtcg tctgagcatg ttgacctgtt cagttgtccc cgcagcccgg ccgcttcgcc
      841 acgtagcgac gtgttgcatg agagccgctg cctgagaaac cgggtaggat gacgattcgt
      901 ctgtatctcg tctgcgacca ccttctcttc cgtaccgggc tcacccggct cctgcgccgc
      961 gagccggatg tgcacgtcgt cggcgagtgc ccgctcgggc tcgacgcggc ggaccaggtg
     1021 cgggtcgcgg acgccgacgt cgtcctgctg tccgtggaca cgccgaacac cgcaagcgcg
     1081 ccggcggcgc agcggcttta ccgcgacctg atgaacgtcc cggtcgtgct cctgctcatg
     1141 ggcgatgacg gcgacgacgc gctcgccgcg ctgcgcctcg gagcacgcgg cgtgctcgac
     1201 aagcacaccg acggcacccg cctggtgaac gcgcttcgcc aggcggtcgt cggagaaatc
     1261 accgtgacgc cgcggctcgc cacgcgcctc gtcgccgagt acgcagccat cgccaccggg
     1321 cgcaaacc
//
LOCUS       DASUCJ010001108         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_684488, whole genome shotgun sequence.
ACCESSION   DASUCJ010001108 DASUCJ010000000
VERSION     DASUCJ010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684488"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1083)
                     /locus_tag="VFU90_13020"
     CDS             complement(<1..>1083)
                     /locus_tag="VFU90_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEU5480758.1"
                     /translation="KGNIQPLKLAFVVAIVLATVATIVFFERAQRRIPIYYARRTVGR
                     RIYGGQTAHLPLRVNTSGTIPPIFASSLLMFPATLANFKIPGMSALQGMLNRGDWAFN
                     TFYVLLIIFFCHFYTAVTFQPVDVADNLKKQQAFIPSIRLGKQTADYIDYVLTRITFG
                     GSLYVAVVCIVPSIILQLLTMVFKPLDELRKEGEQGQRKIDQYTRYGTIILSLIQGFG
                     IAMSLEGMNNGDLSDSAAVGDVVTHAGWGFRLMTMLTLTTGTAFMMWVGEQITERGIG
                     NGISLLIFAGIVDGVPDGIAGYFATNKGNIQPLNLAAVVAITLATVATIVFFERAQRR
                     IPIYYARRTVGRRIYGGQTAHLPLRAN"
BASE COUNT          183 a          318 c          392 g          190 t
ORIGIN      
        1 gttcgcgcgt agcggcaggt gcgcggtctg tccgccgtag atgcgtcggc cgacggtgcg
       61 gcgcgcgtag tagatgggga tgcgccgctg cgcgcgctcg aagaagacga tcgtggccac
      121 ggtcgccagc gtgatggcga cgacggcggc caggttgagc ggctggatgt tgcccttgtt
      181 ggtcgcgaag tagcccgcga tgccgtcggg cacgccgtcg acgatgccgg cgaagatgag
      241 cagcgagatg ccgttgccga tgccgcgctc ggtgatctgc tcgccgaccc acatcatgaa
      301 tgcggtgccg gtcgtgagcg tgagcatcgt catcaggcgg aagccccagc ccgcgtgcgt
      361 cacgacgtcg ccgacggccg cagagtcgga caggtcgccg ttgttcatgc cctcgaggct
      421 catcgcgatg ccgaagccct ggatgagcga gaggatgatg gtgccgtagc gcgtgtactg
      481 gtcgatcttg cgctggccct gctcgccctc cttgcggagc tcgtcgagcg gcttgaacac
      541 catcgtcagc agctgcagga tgatgctcgg cacgatgcac acgacggcga cgtagagcga
      601 cccgccgaac gtgatgcgcg tgagcacgta gtctatgtag tcggcggtct gctttcccag
      661 tcggatgctc gggatgaacg cctgctgctt cttcaggttg tcggccacgt cgaccggctg
      721 gaacgtgacg gccgtgtaga agtgacagaa gaagatgatg agcaggacgt agaaggtgtt
      781 gaacgcccag tcgccgcggt tgagcatccc ctgcagcgca gacatcccgg ggatcttgaa
      841 gttggcgagc gtcgccggga acatgagcag cgacgacgcg aagatcggcg gaatcgtgcc
      901 gctcgtgttc acgcgtagcg gcaggtgcgc cgtctgtccg ccgtagatgc gccggccgac
      961 ggttcgcctc gcgtagtaga tggggatgcg ccgctgcgcg cgctcgaaga agacgatggt
     1021 ggcgaccgtc gcgagcacga tggcgacgac gaaggccagc ttcagcggct ggatgttccc
     1081 ctt
//
LOCUS       DASUCJ010001109         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1219490, whole genome shotgun sequence.
ACCESSION   DASUCJ010001109 DASUCJ010000000
VERSION     DASUCJ010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219490"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFU90_13025"
     CDS             complement(<1..294)
                     /locus_tag="VFU90_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480759.1"
                     /translation="MSVASAAGAYTVGNSAFFRYIVRLTELGLPLSHLDQEAVDILAA
                     IPHAFDQENEMPPTPGRGWRIVPAQGELDWPVLEATPGRLHSAFERARGILWTH"
     gene            complement(343..1434)
                     /locus_tag="VFU90_13030"
     CDS             complement(343..1434)
                     /locus_tag="VFU90_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU5480760.1"
                     /translation="MADAATTEYRLAAESKLRKFVAAALERSGVAPAHAAVVADVLVA
                     ADLRGIESHGVARLESYYIGRIRGGHLNPKPEYRVVRETETTIVCDGDNGLGHPVAKM
                     TMERVLEKAATHGSAFGAVRNSNHFGIAGYYAMMALERDMIGMASTNSVKYGAPTHGR
                     DVLLGTNPLAFAVPAGTEPPFVLDFATTTVPKGKLEVYKRKTKPLLPGWAIDRDGRET
                     LDPDEALRGALLPLGGFGVAGGGHKGYGLGLLVDILCGVLSGGAFGPTLPLVTDVKQG
                     AISHWFGAFRVDGFRDVAAFKADMDRELRYFKDSAKVPGEDRIYVAGEPEFEMTEHHR
                     RHGVPVHAKVWDGLTKLSEELGIPLETVP"
     gene            1505..>1913
                     /locus_tag="VFU90_13035"
     CDS             1505..>1913
                     /locus_tag="VFU90_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5480761.1"
                     /translation="MIEPAPLGFSGADRPSADAFNRCIRCGLCLPTCPTYLETLTETS
                     GPRGRISLIKAVDEGRLGLLDDGFVGQMSECLDCRACEAACPSGVPYGELLDAARTQI
                     RRARAPDERPRDRLVRRAALALLARPGLLRSVAA"
BASE COUNT          304 a          664 c          608 g          337 t
ORIGIN      
        1 gtgagtccac aagatgccgc gcgcgcgttc gaacgcgctg tggagtctgc cgggcgtcgc
       61 ttccaacacc ggccagtcga gttcgccctg cgccggaacg atgcgccatc cacgcccggg
      121 ggtcggcggc atctcgtttt cctgatcgaa cgcgtgcgga atcgccgcca ggatatcgac
      181 cgcttcctgg tcgagatgcg agagcggcaa cccgagctcg gtcaagcgca cgatgtagcg
      241 aaagaacgcc gagttgccga cggtgtacgc gcccgcggcc gaagcaacgc tcacgtggat
      301 caaatcgaga tcggggtctc cccggcgcga cgacacgggc ggctacggta ccgtctcgag
      361 cgggatgccg agttcctcgc tcagtttcgt caatccatcc cacactttcg cgtgcacggg
      421 cacgccgtgc cggcggtggt gttcggtcat ctcgaattcg ggttcgccgg cgacgtagat
      481 tcggtcttcg cccggaacct tcgcgctgtc tttgaagtag cgcagctcgc ggtccatgtc
      541 ggccttgaac gccgcgacgt cgcggaagcc gtccacgcga aaggctccaa accagtgcga
      601 gatggcgcct tgctttacgt ccgtcaccag cggaagcgtg ggtccgaacg cgcctcccga
      661 cagcacgccg cacaggatat cgaccagcaa tccgaggcca tatcccttat gaccgccgcc
      721 cgccaccccg aatccgccca gcggcagcag cgctccgcgc agcgcttcgt cgggatccag
      781 cgtttcgcgc ccgtcgcgat cgatcgccca gcccggcagc agcggtttcg tcttgcgttt
      841 gtagacctcg agctttccct tcggaaccgt cgtcgttgca aagtcgagta cgaacggcgg
      901 ctccgtaccg gccggtaccg caaacgccag cggattggtt ccgagcagca cgtcgcggcc
      961 gtgggtcggc gcgccgtact tcaccgaatt cgtcgaggcc atgccgatca tatcgcgctc
     1021 gagcgccatc atcgcgtagt agcccgcgat cccgaagtga ttcgaattgc gaaccgctcc
     1081 gaaggccgac ccgtgcgtcg ccgccttctc gagaacgcgt tccatcgtca tcttggcgac
     1141 cggatggccc aagccgttgt ctccgtcgca cacgatcgtc gtctcggttt cgcgtacgac
     1201 gcggtactcg ggtttgggat tgaggtgtcc gccgcgaatc cgtccgatat agtacgattc
     1261 gaggcgcgcg acgccgtgcg actcgattcc gcgcagatcg gcggcgacga gcacgtcggc
     1321 gacgaccgcg gcatgggccg gcgcgacccc cgaacgctcg agtgccgcgg cgacgaactt
     1381 cctcagtttt gattcggcag ccagccggta ttcggttgtc gcggcgtcag ccaagaacga
     1441 taaacctccc catgctcttt cgagcggcag aacttgcggt catgcaaagc gaaagctcgc
     1501 ccgcatgata gagccggcac cgttaggctt cagcggcgcc gatcggccct cggcagacgc
     1561 cttcaaccgc tgcattcgct gcggcctgtg cttgccgacg tgccctacct acctcgaaac
     1621 gctgaccgaa acctcggggc cgcgcggccg gatcagcttg attaaggccg tcgacgaggg
     1681 gcggctcggt ctgctcgacg acggcttcgt cggacagatg tcggagtgcc tggactgccg
     1741 cgcttgcgag gctgcctgcc cctccggggt gccctacggc gagctgctcg acgcggcgcg
     1801 cacgcagatt cggcgcgcgc gcgcgccgga cgaacgcccg cgcgaccggc tcgttcgccg
     1861 tgccgcgctc gcgctgcttg cgcgtcccgg gctcttgcgg tccgtcgccg cgc
//
LOCUS       DASUCJ010001110         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_317275, whole genome shotgun sequence.
ACCESSION   DASUCJ010001110 DASUCJ010000000
VERSION     DASUCJ010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317275"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFU90_13040"
     CDS             <1..903
                     /locus_tag="VFU90_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480762.1"
                     /translation="GATVHPGLLVRGLRRVALQMGVRIYENTPMRRLERDLPPVVRTP
                     QGAVRAGKVVLALNASMPTEFPRFRRAVILVSSDMIITDPCPGRLAQIGLRDGVAVMD
                     SRIFVYYYRTTPEGRLMLGKGGNTFAFGSRMLKIFDEPSPYRRALADSLREFFPELAD
                     VPISASWNGASERSRTGLPFFAHLDRHPSIVYGFGYSGNGVGPTWIGGQFLSSLVLGL
                     DNEWTRSPMAKGPLGRFPPEPFRYVGSIMVRNAVRRKERDEDLGRPPSWIDARLAKLA
                     AAAGKSDKGIHAVAGVPSADRGKP"
     gene            complement(972..>1184)
                     /locus_tag="VFU90_13045"
     CDS             complement(972..>1184)
                     /locus_tag="VFU90_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480763.1"
                     /translation="AYERSSAMPPAIIFTLPPEVRGNETLTDHLRTLGATGFIDDSPR
                     PGAASLIEAALRARAAEEDASKPAVA"
BASE COUNT          189 a          385 c          398 g          212 t
ORIGIN      
        1 ggcgcgaccg tgcatccggg cctgctggtt cgcggcctgc gtcgggtcgc gctgcagatg
       61 ggcgtgcgca tctacgagaa cacgccgatg cgccgactcg agcgcgacct accgccggtc
      121 gtgcgcaccc cgcagggtgc cgtgcgtgcc ggaaaggtcg tgctggcgct caacgcgtcg
      181 atgccgaccg agttcccgcg attccgtcgc gcggtcatac tggtgtcgag cgacatgatc
      241 atcaccgacc cgtgccccgg tcgcctcgcg cagatcggct tgcgtgacgg cgtcgcggtg
      301 atggactccc gcatcttcgt ctactactac cgcacgaccc ccgaaggccg gctcatgctg
      361 ggcaaaggcg gcaacacctt tgcgttcggg tcgaggatgc tgaagatttt cgacgagccg
      421 tcgccttacc ggcgcgcgct tgccgacagc ctgcgtgaat tcttcccgga actggccgac
      481 gttccgatca gcgcgagctg gaatggcgca tccgagcgct cgcggacggg cttgccgttc
      541 ttcgcgcatc tcgaccgaca tccgtcgatc gtctacggct tcggctactc aggcaacggc
      601 gtcggaccga cctggatcgg cggacaattc ctgtcgtcgc tggtgctcgg cctcgataac
      661 gagtggacgc gctcgccgat ggcaaaaggg ccgctcggcc ggtttccgcc cgagccgttt
      721 cgctatgtcg gctcgatcat ggtgcgcaac gcggtccgcc ggaaggagcg tgacgaggat
      781 ctcggccggc cgccgagctg gatcgacgct agactggcaa agctcgctgc cgccgcggga
      841 aagtcggaca aggggattca cgcagtggcc ggggtgccat cggctgaccg agggaagcca
      901 tagtgctgac cgagggatca cgaaagtggc actgcattga tgcgtataat tggtattatg
      961 tcaactcaca gctaggctac ggccggctta ctcgcgtcct cttccgcggc gcgcgcccgc
     1021 agcgccgcct cgatcagact cgcagccccc gggcgcggcg aatcgtcgat gaatccggtg
     1081 gcgccgagcg tgcgcaggtg atcggtgagc gtctcgttgc cacgcacctc cggtggcagc
     1141 gtgaagatga tcgccggagg cattgcggaa gatcgctcgt atgc
//
LOCUS       DASUCJ010001111         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_715056, whole genome shotgun sequence.
ACCESSION   DASUCJ010001111 DASUCJ010000000
VERSION     DASUCJ010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715056"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1547
                     /locus_tag="VFU90_13050"
     CDS             117..1547
                     /locus_tag="VFU90_13050"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEU5480764.1"
                     /translation="MMKNFRVARGLAWQIVLAASIAALPVLATADEPASKDALFGDPA
                     PAPKQSPTGDDSAPASKDALFGNDLGIAPKSPWRGYVRAELADTYASPSHWSKMLVSS
                     EVDAEGAISANVKYHLGARFDYDFVYDANDFYPPEVKRDQRANFMLRENYLDVGAGDW
                     DFRLGRQQIVWGEMVGLFFADVVSAKDLREFILPDFDQIRIPQWAGRAEYFKGDFHFE
                     AIWIPVAGYDDIGKPGAEFFPAVVPPPPGFATRYDNEQFPNRSLAHTDAGLRLSYLKN
                     GWDMSGFYYGSMDAQPTFYRTIVEGPAPAYVYQARHDRIDQVGGTVAKDLGPAVFKAE
                     TVLTHGRSFPSLDPSNVDGVVRQNTLDVIGGLDFTLPADTRLDVQLFNRTFFDHDPTI
                     IYKRNEPGATLLVNHKFGDNVEAEVLWVTSLVRNDWMLRPHVTWRFKRDWQWRFGVDV
                     FDGPPLGLFGEYNDRDRVYSELRYSF"
     gene            1589..>1953
                     /locus_tag="VFU90_13055"
     CDS             1589..>1953
                     /locus_tag="VFU90_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480765.1"
                     /translation="MPLVRVEGVSRRYLLGTEEVWALRDVSLTVERGVFMAIAGPSGS
                     GKTTLLNLIGCIDTPTSGSIVIDGHDVSGRTPDQLAELRARTIGFVFQTFNLLPVLSA
                     RENVEYPLLQMHELSRAERR"
BASE COUNT          347 a          642 c          613 g          351 t
ORIGIN      
        1 gatcgatcgg cggacaggcg aattagacgg cggatacgac acgccgttgc acgcttccct
       61 acaatctgct cattttggct ggctttcggc aatcgcccac cgcagaatcg ggacccatga
      121 tgaagaattt tcgcgtcgcg cgcggacttg catggcaaat cgtgctcgct gcctcgatcg
      181 cggcattgcc ggtgctggcg actgccgacg agccggcgtc gaaggatgcg ctgttcggtg
      241 atcctgcgcc ggcaccgaag caatcaccga cgggtgacga ttccgcgcca gcctcgaagg
      301 acgcgctgtt cggcaacgat ctggggatcg cccccaagtc gccatggcgc ggctacgttc
      361 gcgccgagct ggcggatacc tacgcgagtc cgtcgcactg gtcgaagatg ctggtgagct
      421 ccgaagtcga cgccgaaggc gcgatctccg ccaacgtcaa gtaccacctc ggcgcgcgct
      481 tcgattacga cttcgtctac gacgcgaacg acttctaccc acccgaagtg aagcgcgacc
      541 agcgcgccaa cttcatgctg cgcgagaact acctcgacgt cggcgccggc gactgggatt
      601 tccggctcgg acgccagcag atcgtgtggg gcgagatggt cgggctgttc ttcgccgacg
      661 tggtgtcggc gaaggacctg cgcgaattca tcctcccgga cttcgatcag atccgcatcc
      721 cgcagtgggc tgggcgcgcc gagtatttca agggggattt ccacttcgag gcgatctgga
      781 ttccggtcgc cggctacgac gacatcggca agcccggagc ggagttcttc ccggccgtgg
      841 tgccgccgcc tccggggttc gcgacgcgct acgacaacga gcagttcccg aaccgttcgc
      901 tcgcgcacac cgacgccggc ctgcgcctct cctatctgaa gaacggctgg gacatgtcgg
      961 gcttctacta cggcagcatg gacgcgcagc cgacgttcta ccggacgatc gtcgaagggc
     1021 cagcgcccgc gtacgtgtac caggcgcgcc acgatcgcat cgaccaggtc ggcggtacgg
     1081 ttgcgaagga tcttggcccg gcggtgttca aggccgagac cgtgctcacg cacggccgca
     1141 gcttcccatc gctcgaccct tcgaatgtcg acggcgtcgt gcgccagaac acgctcgacg
     1201 tgatcggcgg gctcgacttc acgctacccg cggatacgcg actcgacgtc cagctcttca
     1261 accgaacgtt cttcgaccac gacccgacga tcatctacaa gcgcaacgag ccgggcgcga
     1321 cactcctcgt caaccacaag ttcggcgaca acgtcgaagc cgaggtgctc tgggtgacga
     1381 gcctcgtgcg caacgactgg atgctgcgcc cgcacgtcac ctggcgcttc aaacgcgact
     1441 ggcagtggcg gttcggggtc gacgtgttcg acgggccgcc gctggggctg ttcggggagt
     1501 acaacgaccg agaccgcgtg tactccgagc ttcgatacag tttctgatcg ggtcggtcct
     1561 ctcgccattg tcgaacggcg aacgcgtcat gcccctggtt cgagtcgaag gagtctcccg
     1621 acggtacctg ttgggtaccg aagaggtttg ggcgctgcgc gacgtttcgc tcactgtcga
     1681 gcggggcgtc ttcatggcga tcgccgggcc atcgggaagc ggaaagacca cactgctcaa
     1741 cctgatcggc tgcatcgaca caccgacgtc ggggtccatc gtcatcgacg gacacgacgt
     1801 ttccggacga accccggacc agctcgccga gcttcgcgcg cgaaccatcg gattcgtctt
     1861 ccagacattc aatctgctgc cagtactttc ggcgcgcgag aacgtcgagt acccgcttct
     1921 gcagatgcac gagctctcgc gggcggagcg tcg
//
LOCUS       DASUCJ010001112         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1509963, whole genome shotgun sequence.
ACCESSION   DASUCJ010001112 DASUCJ010000000
VERSION     DASUCJ010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509963"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFU90_13060"
     CDS             complement(<1..324)
                     /locus_tag="VFU90_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480766.1"
                     /translation="MQIIRKNFTLKLLALGLAIVGWAYFRFASNPIVAAARFAQQISV
                     PIAAVNLPVGLVAHFTEREAVVTVEGRRGDPAIKPDDVKAVLDLSGKGPGVYNVPVTL
                     VAPAIA"
     gene            complement(293..1189)
                     /gene="cdaA"
                     /locus_tag="VFU90_13065"
     CDS             complement(293..1189)
                     /gene="cdaA"
                     /locus_tag="VFU90_13065"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU5480767.1"
                     /translation="MLSSAFSYVGINDVIDVIATSILIYYLLLLIRGTRAVQILMGIL
                     VIFALLGLANVLHLYLLVTILQLIVVGSIVTIPIVFQPELRRALEQIGRGGMFRLDSD
                     DKVAGGWTRPEDRAIAALSQAAFVLSRDERGALIVIEQQSGLKEFCETGTTLEARLSA
                     ELLLAIFTPRSPLHDGATIVRENVIVAAGCFLPLAEHSLRGVRLGTRHRAALGLTEQT
                     DAVVVVVSEESGAVSIARNGKLSRPVEEEQRLTKMLLAITRPPRRERRLRNDLVSHLR
                     SRLAPPRERENPRADHPQELHT"
     gene            complement(1182..1736)
                     /locus_tag="VFU90_13070"
     CDS             complement(1182..1736)
                     /locus_tag="VFU90_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480768.1"
                     /translation="MRWATARSDGILDDSRREAVVAAVAARGGRCTWRTSDRCRRSYA
                     LLEFPEGEASFEPLSAVAANGATVYESPVIALAVFPAVPEALPPLLRALGGTGRPAGV
                     LRCDACGDGAAVEWDIERTPASVVLGLVDVELARFASGRTIELLTPLPPAWRARIARD
                     GLRAPEISSDRILEALVERAELRA"
     gene            1773..2657
                     /gene="rsgA"
                     /locus_tag="VFU90_13075"
     CDS             1773..2657
                     /gene="rsgA"
                     /locus_tag="VFU90_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5480769.1"
                     /translation="MRVEDVRRALVVATGKNSAWISVDGEPLPRIAQLRRMRGKRSMP
                     VPGDVVDVQILEDGAVVERVYPRGFTLERRTAGGRSKTMAANVDMLVTVTALANPSPR
                     LAVLDQLLAFAELERVGAVVVFTKPDLGDSAELRRLTETYAALGYPTIVTNPKRGENV
                     EALRALIRTHRAMLVGNSGVGKSSIFRALGGEGAVGEVSRYGVGRQTTTAARLYRLGS
                     GFLIDSPGVNEFGLGRVAAPELTAAFREMREPAAGCRFADCTHLREPDCGVLAAVAEG
                     RIAESRYASYEKILTQPV"
     gene            2654..3061
                     /gene="rpsT"
                     /locus_tag="VFU90_13080"
     CDS             2654..3061
                     /gene="rpsT"
                     /locus_tag="VFU90_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5480770.1"
                     /translation="MTGGPALDTARGRLVYFWPFSRIYDESELSLPNIKAAEKWSRQS
                     EKRSTRNKDASTRLKTLFKKAASSGDAESAKSVESAYDKAANKGVIHPNKAARKKARL
                     ARALRSAAVAAPAKPVRAKAAKSSKSGGKTSKK"
BASE COUNT          574 a         1092 c         1010 g          472 t
ORIGIN      
        1 cgcgatggcc ggcgctacca gggtcacggg aacgttgtag acgcctggac ctttgccgga
       61 tagatcgaga acggccttca cgtcgtccgg tttgatcgcc gggtccccgc gccggccctc
      121 gaccgtgacg acggcctcgc gctcggtaaa atgtgcgact agcccgaccg gcaggtttac
      181 ggcggcgatc gggaccgaga tttgctgcgc gaagcgtgcg gccgcgacga tcggattgct
      241 ggcgaagcga aaatacgccc agccgacgat cgccagaccc agcgcgagca gcttaagtgt
      301 gaagttcttg cggatgatct gcacgtgggt tttcccgttc gcgcggcggc gccagacgcg
      361 accggaggtg cgaaaccagg tcgttgcgga gacggcgttc gcgccgcggc ggacgcgtga
      421 tcgccagcag catcttcgtc agccgctgct cctcctcgac cggacgcgag agtttcccgt
      481 tacgggcaat cgaaacggcg ccgctttcct ccgaaacgac gaccacgacc gcatcggtct
      541 gctcggtgag tccgagcgct gcccggtggc gggtgcccag ccgcacgccg cgcagcgaat
      601 gttccgccag cgggaggaag cagccggcag cgacgatgac gttttcgcgg acgatggtgg
      661 cgccgtcgtg gagcggcgaa cgcggcgtga aaatcgccag cagcagctcg gccgaaaggc
      721 gtgcttcgag cgtcgtcccc gtttcgcaaa actctttcag cccgctttgc tgttcgatca
      781 cgatcagcgc gccgcgctcg tcgcgcgata gcacgaaggc cgcttgcgac agcgcggcaa
      841 tcgcccggtc ctccggccga gtccaacctc ccgccacctt gtcgtcgctg tcgaggcgga
      901 acattccgcc gcgcccgatt tgttcgagcg cgcggcgcag ctccggctga aagacgatcg
      961 gaatggtaac gatcgaaccg acgacgatca gctgaagaat cgtcaccagc agatacaagt
     1021 gcaggacgtt tgcgagcccc agaagcgcga agatcaccag gattcccatc agtatctgca
     1081 ccgcgcgcgt gccccggatc agcagaagca ggtagtagat gagaatgctc gtcgcgatga
     1141 cgtcgatgac gtcgttgatg ccgacgtacg agaacgcgct gctaagcacg cagttccgcc
     1201 cgttcgacga gcgcctccag aatgcgatcc gaagaaatct ccggcgcgcg cagtccgtcg
     1261 cgggcgatgc gcgcgcgcca cgccggcggc agcggcgtca ggagctcgat cgtgcgtccg
     1321 ctcgcaaaac gcgccagctc gacgtccact agcccgagca ccacggaggc cggcgtccgt
     1381 tcgatatccc actccaccgc cgcaccgtcc ccgcacgcat cgcagcgcag cacgccggcc
     1441 ggtcggccgg tgccgcccaa ggcacgcaac agcggcggca aggcttccgg caccgcaggg
     1501 aaaactgcaa gggcgatgac cggcgattcg tacacggtcg cgccgttagc cgccaccgcc
     1561 gacagcggct cgaacgaggc ctccccctcg gggaattcca gcagagcgta cgagcgtctg
     1621 caccgatccg acgttcgcca cgtgcagcgt ccgccgcgcg ccgccaccgc cgccaccacg
     1681 gcttcgcgtc gcgagtcgtc gaggatgccg tcggaccgcg cggtagccca tctcacgtcg
     1741 ataccttcgt taggcgtacc cgcggctcac tcttgcgcgt agaggacgtt cgccgcgctc
     1801 tggtcgtcgc gaccggcaaa aactcggcgt ggatctcggt cgacggcgag ccgctgccgc
     1861 ggatcgcgca actccgccgg atgcgcggga aacgctccat gcccgtgccg ggcgacgtgg
     1921 tcgacgttca aatactcgaa gacggcgcgg tcgtcgaacg cgtctatccg cgaggattta
     1981 ccctcgagcg ccgtaccgcc ggcggacgtt cgaaaacgat ggccgccaac gtcgacatgc
     2041 tcgtgaccgt aacggcgctt gccaatccgt cgccgcggct ggccgtattg gaccagctgc
     2101 ttgcctttgc cgagctcgag cgggtggggg cagtcgtcgt gttcaccaaa cccgacctcg
     2161 gcgattcggc cgaattgcgc aggctgaccg aaacctacgc cgccctcggc tacccgacga
     2221 tcgtcaccaa ccccaaacgc ggcgaaaacg tcgaagccct ccgggccctc atccgcacgc
     2281 accgtgcgat gctggtaggc aattccggcg tcggcaagag ttcgatcttc cgcgcgctcg
     2341 ggggcgaagg tgccgtcggc gaggtttccc gttacggcgt cggccgccaa acgaccacgg
     2401 ccgcacgcct ctaccgcctc ggttcgggct tccttataga cagccccggc gtcaacgagt
     2461 tcggcttggg cagggtggcg gcgccggagc ttaccgccgc cttccgcgag atgcgcgagc
     2521 cggccgccgg ctgccgtttt gccgactgta cgcacctccg ggaaccggac tgcggggtgc
     2581 tggctgccgt cgccgaagga cgcatcgccg aaagccggta cgccagctac gaaaagattc
     2641 tcacccagcc ggtttgaccg gcgggccggc gctggacact gcgcgcggcc ggctggtata
     2701 cttctggccg ttctcccgca tctacgacga aagcgagcta tccttgccca acatcaaagc
     2761 cgcggaaaaa tggtcgcggc agtcagagaa gcgctccacc cgcaataagg acgcctcgac
     2821 gcgcctgaag acgctcttca agaaagccgc ctcctcgggc gatgcggaat ccgctaagag
     2881 cgtcgagagt gcctacgaca aggccgcgaa caaaggcgtc atccatccga acaaggcagc
     2941 ccgcaagaaa gcacggctcg cccgcgcgct tcgcagcgcg gccgtagccg ctcccgccaa
     3001 gccggttaga gccaaggcag ccaaatcgag caagtcgggc gggaagacgt ccaagaagta
     3061 accccgccgg accgcccgga tccggcggtc gacccgcgcg aaaccttcct cacgacgatg
     3121 ggccgcgagc tccaccgcgc ggggattg
//
LOCUS       DASUCJ010001113         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_990066, whole genome shotgun sequence.
ACCESSION   DASUCJ010001113 DASUCJ010000000
VERSION     DASUCJ010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990066"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..677)
                     /locus_tag="VFU90_13085"
     CDS             complement(162..677)
                     /locus_tag="VFU90_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5480771.1"
                     /translation="MKRVRSGRPQPGEYADYAQADIDFVTGDDAVEALESSREKTLAL
                     FSGNIADRPYAPGKWTLKEILGHLVDDERIFAYRALCVARGEPLALPGFDEKLYVTNA
                     GFEERRRDDLLAEYKTVRQSTIAFCSGLTEEQWMRRGIVNGYPASVRGLAFHIAGHEL
                     HHLRIVRERYS"
     gene            complement(674..931)
                     /locus_tag="VFU90_13090"
     CDS             complement(674..931)
                     /locus_tag="VFU90_13090"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEU5480772.1"
                     /translation="MIIERYRNGDALPVYRRFRERGRLAPEGLQYVNSWVVPDLKTCY
                     QVMQTDDRALLDQWIAAWSDLVDFDVIPVITSADAAARVAE"
BASE COUNT          176 a          328 c          331 g          174 t
ORIGIN      
        1 cccggtcccg ctgcgcggga ccgacctctc cccgcgggag aggttgttgg accgcgcgcc
       61 cggattggcc tctcccgcgg aaagaggtca ggtgtcgcgg acctctcccg tcgggagagg
      121 tcgccgcgaa gcggcgggtg aggggcccac gaggaagatc gtcacgaata acgctccctg
      181 acgatccgca gatgatgcag ctcgtgtccg gcgatgtgaa aggcgaggcc gcgcaccgat
      241 gccggatagc cgttgacgat gccgcggcgc atccactgct cctcggtcag cccgctgcag
      301 aaggcgatcg tcgactgacg aaccgttttg tactcggcga ggagatcatc cctccttcgc
      361 tcttcgaaac cggcgttggt cacgtacagc ttctcgtcga atccgggcag cgcgagcggc
      421 tcgccgcggg caacacacaa agcgcgatac gcaaagatcc gctcgtcgtc gacgaggtgc
      481 ccgaggattt ccttgagggt ccatttgccg ggagcgtacg ggcggtcggc gatgttgccg
      541 gagaacagcg cgagcgtttt ctcccgcgac gattcgagcg cttcgaccgc gtcatcgccc
      601 gtgacgaagt cgatgtccgc ttgcgcgtaa tccgcgtatt cgccgggttg cggtcggccg
      661 gaccggacgc gcttcattct gcgacccgcg ccgcggcgtc ggcggaggtg atcaccggaa
      721 tgacgtcgaa atcgacgaga tcgctccacg cggcgatcca ctggtcgagc agcgcgcgat
      781 cgtcggtctg catcacctgg tagcacgttt tcagatcggg cacgacccag ctgttgacgt
      841 actgcaggcc ctcgggcgcg aggcgtccgc gttcgcgaaa acgccgatag accggcagcg
      901 cgtcgccgtt gcgatagcgc tcaatgatca tgtacagcac gccgcaactc tatcggatag
      961 actccgcggc cgatgcaaac gattcgtttc ggccgcacaa acgcccgcg
//
LOCUS       DASUCJ010001114         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_440045, whole genome shotgun sequence.
ACCESSION   DASUCJ010001114 DASUCJ010000000
VERSION     DASUCJ010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440045"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFU90_13095"
     CDS             complement(<1..420)
                     /locus_tag="VFU90_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5480773.1"
                     /translation="MADVALRHAIESPVPVRERLHALDDSALVTEFLSGEERAFQVLV
                     ERYQTRLLNFIYRTIGDRERAEDLVQEVFIRVYRHLHRFDRSKKFSTWVYTIASNLAK
                     NELRNRSRSPLVFFQTIRKNWQDDDRPLQFEDHVSRPD"
     gene            complement(741..1292)
                     /locus_tag="VFU90_13100"
     CDS             complement(741..1292)
                     /locus_tag="VFU90_13100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5480774.1"
                     /translation="MADPGQVSTTRRYSGHVVNLDVDTVRFPDGSVGELEMVRHPGAS
                     LVLPFLSDPAGEDPQVLLIRQYRYAARGFVIECPAGRLEPGESPEACARRELREEAGC
                     TAERLEHVYTFYTTPGFTDERIHAFMASGLSRGEARLEADEFVETVTVSLSRALEMVR
                     DSEIVDAKTALTLLYVAGFRLGS"
     gene            complement(1296..>2630)
                     /locus_tag="VFU90_13105"
     CDS             complement(1296..>2630)
                     /locus_tag="VFU90_13105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5480775.1"
                     /translation="PYRFPRFEIVTLDNGLRVIVAPVRTLPVVTALLLVDAGASTEPA
                     GRDGVANLTARGLLEGTLQRDGNALTEQFERLGASVFSGADWDTANAGVTVLREHVEP
                     AIALLGELALAPSFPERDIARLRAERLADLLQMRTEPRGLADEMFARFVYDPFSRYAR
                     PEEGGSDTVATLTRDDVRRFYTERYRPRGSTFVLVGDVSVDEGVSIATRTFGGWDGVV
                     SPTAPPADAPARRTRAAHLVRKADAPQSELRVGHIGLPRRHPDYFRVMVMNAVLGGLF
                     SSRINLNLREAHAYTYGAFSGFAWRRGAGPFVVSTAVENDVTADAAREILLEIDRIRA
                     APISADELSLATSYLGGVFPIKYETTDAIALALAALAAYELPIDYFDTYRDAVRAVST
                     ADVLDAAQRYLHPDALQVVVVGDASQIRAPLAAMALGPMLEYGADGVEISGD"
BASE COUNT          472 a          897 c          843 g          418 t
ORIGIN      
        1 gtcgggccgc gatacgtgat cctcgaattg cagcggccga tcgtcgtcct gccagttctt
       61 ccgaatcgtt tggaaaaaca ccagcggact cctggaacga ttgcgcaact cgttcttcgc
      121 caggttcgac gcaatggtgt aaacccacgt cgagaacttc ttcgagcgat cgaaccgatg
      181 caggtgccgg tacacccgga tgaagacctc ttgcacgaga tcttccgcgc gctcgcgatc
      241 cccgatggtg cggtagatga agttgagcag ccgggtctgg tagcgttcga ccagcacctg
      301 gaaggcgcgc tcctcgccgc tcaggaactc ggttaccagc gccgagtcat ccagagcatg
      361 cagtcgctca cgtaccggaa ccggcgactc gatggcgtgc ctcagtgcaa cgtcagccat
      421 tgctgctccc caatcattga agggttcgcg ctctccgaga gacgaagcgc caagcggccg
      481 tgcacacatt ccgtcccgtc actcgaaaaa gcatgccccg tgcccgccga accgcttcaa
      541 ctgaataacg cctaagtcac tctgatacca agacttccat gattgctgcg gtaaatcgag
      601 agaccggccg tgccctgtcg tcgtgtctac tttctgagac gaaaagcccg atactatggg
      661 acacgttcgc cgactgtggg gtagtagtgc gccgcacgag cacgactggt ttgaccaacc
      721 cacgccgcga acagttgccg tcaggaaccc agccgaaaac cggcgacata caacagggtc
      781 agcgcggttt tggcgtccac gatctcgctg tcgcgcacca tctcgagcgc gcgcgacagt
      841 gacacggtca ccgtctcgac gaactcgtcg gcctccaggc gcgcttcgcc acgcgacagg
      901 ccggacgcca tgaacgcgtg gatgcgctcg tccgtgaagc ccggcgtcgt gtagaacgtg
      961 tacacgtgct cgagccgctc ggccgtgcac ccggcttctt cccggagctc gcggcgtgca
     1021 cacgcctcgg gcgactcgcc cggctccagt cgtcctgccg gacactcgat cacgaagccg
     1081 cgggctgcgt aacggtattg ccgaatgagc aggacctgcg ggtcttcacc ggccggatcg
     1141 ctcaggaacg gcagcacgag actcgcaccg gggtgacgaa ccatctcgag ctcgcccacc
     1201 gagccatcgg gaaagcgaac cgtgtcgacg tcgaggttga ccacgtggcc gctgtagcgt
     1261 cgcgtcgtcg agacctgtcc ggggtcggcc atgcgtcagt ccccggagat ttcgacgcca
     1321 tcggcgccgt actcgagcat cggccccagc gccatcgcag cgaggggcgc cctgatttgc
     1381 gacgcgtcgc cgacgaccac gacctgcagc gcatccgggt gcaggtagcg ttgggcagcg
     1441 tccagcacgt cggcggtgct cacggcgcgc accgcgtcgc ggtacgtgtc gaagtaatcg
     1501 atgggcagct cgtacgcggc gagcgcggca agcgcgagcg cgatggcgtc cgtcgtttcg
     1561 tacttgatcg gaaacacgcc gcccaggtag ctcgtagcca acgacaattc gtcggcggaa
     1621 atgggcgcgg cgcgaatacg atcgatctcc aacagtattt cgcgcgccgc gtccgcggtg
     1681 acgtcgttct cgacggcggt cgacacgacg aacggcccgg cgccgcgccg ccaggcgaag
     1741 ccggagaacg ccccgtacgt gtacgcgtgc gcttcgcgca ggttcaggtt gatgcgcgac
     1801 gagaacagtc cgcctaacac agcgttcatc accatcaccc gaaaatagtc cgggtgccgg
     1861 cgcggcagtc cgatgtgccc aacgcggagc tccgactgcg gcgcatccgc cttgcgcacg
     1921 aggtgcgccg cccgcgtgcg gcgcgccggc gcatcggcgg gcggtgcagt cggcgacacg
     1981 acaccatccc agccgccgaa cgtgcgcgtc gcgatcgaaa caccttcgtc gacgctcacg
     2041 tctccgacga gcacgaacgt cgatccgcgc ggccgatagc gctcggtgta gaatcgccgc
     2101 acatcatccc gggtcagcgt ggcgacggtg tccgatccgc cttcttccgg ccgcgcgtag
     2161 cgcgagaacg ggtcgtacac gaagcgggca aacatctcgt cggccaggcc gcgcggctcc
     2221 gtacgcatct ggagcagatc ggcgagtcgc tcggcgcgca gccgcgcgat gtcgcgctcg
     2281 ggaaacgacg gcgcgagcgc cagctcgccg aggagcgcga tggccggttc gacgtgttcg
     2341 cgcagcaccg tcacgccggc gtttgccgtg tcccagtccg caccggagaa cacgctcgcg
     2401 cccaaccgct cgaattgctc ggtgagcgcg ttgccgtcgc gctgcagggt tccctcgagg
     2461 agcccccgcg ccgtgagatt cgccacgccg tcgcgacctg ccggctcggt cgacgcgccg
     2521 gcgtcgacga gcagcagcgc cgtcacgacc ggcagcgtgc gcacgggcgc gacgatcacg
     2581 cgcaggccgt tgtcgagcgt cacgatttcg aagcgcggaa agcggtaggg
//
LOCUS       DASUCJ010001115         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1204037, whole genome shotgun sequence.
ACCESSION   DASUCJ010001115 DASUCJ010000000
VERSION     DASUCJ010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204037"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFU90_13110"
     CDS             <1..604
                     /locus_tag="VFU90_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5480776.1"
                     /translation="EARAMLDGIEPAVSAGKLACVLAQFPNGFRPGPAREDRVRRVVE
                     SLAAVPVVVEFRNREWQRPETLELVRSLGASYCNVDMPRLERLPAPSSDVAGDVGYVR
                     FHGRNAATWWRGTNVTRYDYLYSVEELVPWTDRIAEIEAQTRNTYVFFNNHANGQAPR
                     NAELIESLLAQRYGSAAAATVARAEGGNPEQTPLPGIPGR"
     gene            601..1338
                     /locus_tag="VFU90_13115"
     CDS             601..1338
                     /locus_tag="VFU90_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5480777.1"
                     /translation="MRAVITAGGRLEAEYADAAGTGVKALAPVRGVTMLDRIIDALRG
                     AGATRIAVVGGEEVRSACAHRVEAVVDESASGSENVLRALSAWPDDDGRPLLYATSDL
                     PYVTAAAVGDFVRRAPAATIAIAVAEHRDFEARFPGAPPFGITLAREKIVNGGIFSIP
                     AGKAATLASAAATFFEARKRPWRMATLVSPLAVLKFAAGRLSIGDLERVAQKMLDVPA
                     TAVRNCAPELGYDADTFFEYHYACRND"
     gene            1319..>1974
                     /locus_tag="VFU90_13120"
     CDS             1319..>1974
                     /locus_tag="VFU90_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480778.1"
                     /translation="MRAETTERVPSALRLGLFWLGIQAVWGALLGISLQARTLELAAG
                     NALVAYGRLATIGALVAALVQIAVGPWSDRRRARGSRRIEFYVAGGIGGAIAIVFFYG
                     ARNFAALSFAFVGIQAALNFAIGPYQAIIPDAMPRSRFGIASSWMAALQSAGNAIGAL
                     CAAFVADLRILGGVLAALLVGTGAATWAHVRGLNLQPQPARVPLRVTRAFVDLFVSRA
                     L"
BASE COUNT          296 a          640 c          700 g          338 t
ORIGIN      
        1 cgaggcacgg gcgatgctcg acggcatcga gccggcggtt tcggccggga agctcgcctg
       61 cgtcctggct caattcccga acgggtttcg cccaggtccc gcgcgcgagg accgcgtgcg
      121 gcgggtcgtc gagtcgctgg ccgccgtgcc ggtggtcgtc gagtttcgta accgcgagtg
      181 gcagcgcccg gaaaccctgg aactggtgcg gtcgctgggc gccagttact gcaacgtcga
      241 catgccgcgg ctggaacggc tccccgcgcc gtcgtccgac gtggcaggcg acgtcggtta
      301 cgtgcgcttt cacggacgga acgcggccac gtggtggcgc ggcaccaacg taacgcggta
      361 cgactacctc tattcggtcg aggagctcgt gccctggacg gaccggattg ccgaaatcga
      421 ggcgcaaacg cgtaacacct acgtgttttt caacaaccat gccaacggcc aggcgccgcg
      481 caacgccgag ctgatcgagt cgctgctcgc gcagcgctac ggttcggcgg cggctgcaac
      541 ggtggcgcgg gcagaaggcg gcaatcccga gcagacgccg ttgccgggaa tacccggccg
      601 atgagggcgg tgatcaccgc gggcgggcgg ctggaggccg aatacgccga tgccgccggt
      661 accggcgtaa aagcgctcgc gccggtgcgc ggcgtaacga tgctcgatcg gatcatcgac
      721 gcgctgcgcg gtgccggggc aacgcgcatc gccgtagtgg gcggtgaaga agtgcgctcg
      781 gcgtgcgcgc accgagtgga agcggtcgta gacgagagcg cgtcgggcag cgaaaacgtg
      841 ctgcgcgcgt tatcggcctg gccggacgac gacggccggc cgctgctcta cgccaccagc
      901 gatctgccgt acgtgaccgc cgcagcggtc ggggatttcg tacgccgcgc gccggcggcg
      961 acgatcgcga tcgccgtcgc cgaacaccgc gatttcgaag cccgcttccc cggggcgccg
     1021 ccgttcggga ttacgctggc tcgcgaaaag atcgttaacg gcgggatatt ttcgattcca
     1081 gccgggaaag ccgcgacgct cgcctcggca gcggcaacgt ttttcgaggc gcgcaagcgg
     1141 ccgtggcgca tggcgacgct ggtgagcccg ctagccgtgc tgaagttcgc cgccggccgc
     1201 ttgagcatcg gcgatttgga gcgcgtcgcg caaaaaatgc tcgacgtacc ggcgaccgcg
     1261 gtccgcaatt gcgcgcccga gctcggatac gatgccgaca cctttttcga ataccattat
     1321 gcgtgccgaa acgactgagc gcgttcccag cgcgctgcgc ctcgggctct tttggctcgg
     1381 gattcaggcc gtgtggggag cgttgctcgg gatttcactc caggcacgga cgctcgaact
     1441 cgccgccggc aacgctttgg tcgcatacgg ccggctcgcg acgatcggtg cgctggtcgc
     1501 cgcgctggtt caaattgcgg tcggcccgtg gtcggaccgc cggcgagctc gcggcagccg
     1561 gcgaatcgag ttctacgtcg ccggcgggat cggcggcgcg atcgcgatcg tcttttttta
     1621 cggagcgcgc aactttgcgg ctctgagctt tgcgttcgtc ggcatacagg ccgccttgaa
     1681 cttcgcgatc ggaccgtatc aagccatcat tccggacgca atgccgcgca gccgattcgg
     1741 gatcgcgtcg tcgtggatgg ccgcacttca aagcgcgggg aacgcgatcg gcgcgttgtg
     1801 tgcggcgttc gtcgccgacc tccgcattct gggcggcgtt ctcgccgcct tgctcgtagg
     1861 cacgggggct gcgacgtggg cgcacgtgcg cggactgaac cttcagccgc agccggcgag
     1921 agttccgctg cgcgttacgc gcgccttcgt cgatttgttc gtttcgcgcg cgct
//
LOCUS       DASUCJ010001116         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1402813, whole genome shotgun sequence.
ACCESSION   DASUCJ010001116 DASUCJ010000000
VERSION     DASUCJ010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402813"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..934
                     /locus_tag="VFU90_13125"
     CDS             209..934
                     /locus_tag="VFU90_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480779.1"
                     /translation="MPPVAWHGKFATSPLATPDFAPTASPVASPIDATLAVASPSIDE
                     PPMPPVPPATPPSTEASDSQLPPTVGALALDVYSTPRALPVEEVAQKPMLESVPQEVE
                     LDEYNPAVSATAPIERDEPLALIDLGTRPMLRRAVPHDELEPDVRAIVDDLYDQARAE
                     LSGTELTLFAPPIVDEPEPAVIEPVAPAVVAPVLVEPEPAVDPSPPLPEPPAAVVDKQ
                     KRARRGWVAAFVTDDHDGKHVTD"
     gene            complement(924..1520)
                     /locus_tag="VFU90_13130"
     CDS             complement(924..1520)
                     /locus_tag="VFU90_13130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5480780.1"
                     /translation="MSELYGGRVFLRPLRTDDWDQWREVRVRCRDWLEKWEPRPEPGS
                     ADPALDREAFRARCGAWDRQRHFDAAYGFGLFLNDGRFAGEVSIGSVQRGPFQMGYIG
                     YWIDQALAGHGYVPEGVVLLMAHAFDALMLHRLEAAIVPHNTSSRRVAEKLELRDEGT
                     AVRFLQIQGVYEDHVRYAITSEEWRARGPALTARFVNR"
     gene            complement(1517..>1883)
                     /locus_tag="VFU90_13135"
     CDS             complement(1517..>1883)
                     /locus_tag="VFU90_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480781.1"
                     /translation="AGKRLGPAEVETAVVGHPQVVEAAAVGVPDELKGEALWVFVVPA
                     RGVDTDDALRQSIAATVVEALGPSFKPAQVRFTKALPKTRSAKVLRRAIRSVVTGAAP
                     GDLSGLEDPASLEAIATAS"
BASE COUNT          307 a          684 c          606 g          286 t
ORIGIN      
        1 gcctttcgtg gagacgttcc gctgtccgac ctgcatcgga gtcttgaacg acccgcgtgc
       61 gcgccgctgc cggcactgtg gtcagcgcat gcgtcggcgc cgaccgcgcg tgttggggga
      121 cgaaacgcgc ctcgggccgc gtcttccgat cgaccgctgg atgctcgaac ggctccagac
      181 gcaatctcgt cgtaggtcac ggcacccgat gccaccggtt gcgtggcacg gaaagttcgc
      241 aacatcaccg ctcgcaacac ccgacttcgc cccgacggcg tcaccggtcg cgtcgcccat
      301 cgacgccacg ctcgcggttg cgtcgccgtc gatcgacgaa ccgccgatgc cgccggtgcc
      361 gcccgcaacg ccaccgtcaa ccgaagcgag cgattcgcaa ctgccgccga cggtcggtgc
      421 gctcgcgctc gacgtctaca gcacgccgcg cgcgttgccg gtcgaagaag tcgcgcagaa
      481 gccgatgctc gagtcggttc cgcaagaggt cgagctcgac gagtacaatc cggccgtcag
      541 cgccaccgcg ccgatcgaac gagacgaacc gctcgcgttg atcgatctcg gcactcgccc
      601 aatgctgcgc cgcgcggtgc cgcacgacga gctggagccc gacgttcgcg cgatcgtcga
      661 cgacctctac gatcaggcgc gcgcggagct gtcgggcacc gaactcacgt tgttcgcgcc
      721 accgatcgtc gacgagccgg agcccgcggt gatcgaaccc gtcgcaccgg ccgtcgtcgc
      781 ccccgtgctc gtcgagccgg aaccggccgt cgatccttcg ccgccgctgc ccgagccgcc
      841 ggcggccgtc gtcgacaagc agaagcgcgc acggcggggt tgggtcgccg cgttcgtcac
      901 cgacgaccac gacgggaagc acgtcaccga ttgacgaagc gagctgtcag cgcgggtccg
      961 cgagcacgcc attcctcgga cgtgatcgcg tagcgcacgt ggtcctcgta gacaccttgg
     1021 atctgaagaa accgcacggc agttccctcg tcgcgtagtt cgagcttctc ggcgacacgc
     1081 cggctcgaag tgttgtgcgg gacgatcgcg gcttcgagcc ggtgcagcat cagcgcgtcg
     1141 aacgcgtgtg ccatcaacag caccacacct tcgggcacat atccgtggcc ggcaagcgcc
     1201 tggtcgatcc aatagccgat gtagcccatc tggaacggcc cgcgctggac gctgccgatg
     1261 ctgacttccc ccgcgaatcg accgtcgttc aagaacaagc cgaagccata cgcggcgtcg
     1321 aagtggcgct ggcggtccca ggcgccgcaa cgcgcacgaa aagcttcgcg atcgagcgcc
     1381 ggatccgcgc tgcccggttc cggacgcggt tcccacttct cgagccagtc gcgacagcga
     1441 acgcggacct ctcgccactg atcccagtcg tcggtgcgga gcggccgcag gaacacgcgg
     1501 ccgccgtaca actcgctcat gatgccgtcg cgatcgcttc gagcgatgcg ggatcttcga
     1561 ggcccgaaag gtcgccgggc gcggctccgg tgacgaccga acggatcgcg cgtcgcagca
     1621 ccttcgcgct gcgcgtcttc gggagcgctt tcgtgaaacg tacttgcgcg ggcttgaacg
     1681 acggaccgag cgcttcgacg accgtcgccg cgatcgactg ccgcaaggcg tcgtcggtgt
     1741 cgacgccgcg cgcgggcacg acgaacaccc acaacgcttc gcctttcagt tcgtcgggaa
     1801 cgccgaccgc cgccgcttcc acgacctgcg gatgcccgac caccgcggtt tcgacttcgg
     1861 ccgggccgag ccgtttcccg gcg
//
LOCUS       DASUCJ010001117         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_822288, whole genome shotgun sequence.
ACCESSION   DASUCJ010001117 DASUCJ010000000
VERSION     DASUCJ010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822288"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..664)
                     /locus_tag="VFU90_13140"
     CDS             complement(41..664)
                     /locus_tag="VFU90_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480782.1"
                     /translation="MRIAAALLALTPLATSALPARASGDLLARMAAVNPTLRTFVATM
                     HANVTLKTFPFLNVALVGTYYHKEPDQTKIVFTSGVPMVAQQFDKLYAHVVSPSRWSQ
                     VYTVSVVSDDGSKTTFRLVPRKRGNVDHIDAVADDATATVDSMRWNYDNGGYAEMHNR
                     YGHAGGDLVVMSQVGHVQEPGYTADITSTIDNYSINVSIPDNVFTPD"
     gene            complement(674..>1001)
                     /locus_tag="VFU90_13145"
     CDS             complement(674..>1001)
                     /locus_tag="VFU90_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5480783.1"
                     /translation="ELLRLRSGEFKRRFAGTAMGWRGAAVLRRNAAVALGNALDRSAI
                     PALALSLNEDPHPMVRGHAAWALGRIGAPSALSALRRRYECEGSESVRAEIRAALEPF
                     ATIDRS"
BASE COUNT          171 a          324 c          326 g          180 t
ORIGIN      
        1 ggtcgacggt caccgccacg atgccgccgg gccggcctcg ctagtcgggc gtaaagacgt
       61 tgtcgggaat cgagacattg atactgtagt tgtcgatcgt ggacgtaatg tccgctgtat
      121 atccgggctc ctgcacgtgc ccgacctgcg acatcaccac caggtcgccg ccggcgtggc
      181 cgtagcggtt gtgcatctcg gcgtatccgc cgttgtcgta gttccagcgc atcgagtcga
      241 cggtagccgt tgcgtcgtcg gcgacggcgt cgatgtggtc gacgtttccg cgcttgcgcg
      301 gaaccagccg gaacgtcgtc ttcgagccgt cgtcggaaac cacgctcacc gtgtagactt
      361 gcgaccatcg cgacggcgaa acgacgtgcg cgtagagttt gtcgaactgc tgcgccacca
      421 tcggcacgcc gctggtgaac acgatcttgg tttgatccgg ttccttgtgg tagtacgttc
      481 cgacgagtgc gacgttgaga aacggaaagg tcttcaacgt cacgttcgcg tgcatcgtcg
      541 ccacgaacgt acgcaacgta ggattgaccg ccgccatccg ggcgagcaga tcgccggacg
      601 cgcgggcggg aagagccgag gtcgcgagcg gggtcaaggc taacaaggcg gcggcaattc
      661 tcatcggatt cctctacgag cggtcgatcg ttgcgaaggg ttccagcgcg gcgcgaatct
      721 cggcgcgaac gctttcgctg ccctcgcact cataacgccg ccgtaacgct gagagcgcgc
      781 tgggagcgcc gatgcgcccc agggcccagg ccgcatgtcc gcgcaccatc ggatgcggat
      841 cctcattgag gcttaacgcc agcgccggaa tcgccgaacg gtcgagcgcg tttccgagcg
      901 cgaccgccgc gttgcgccgc agcaccgccg cgccgcgcca gcccatcgcg gttccggcga
      961 accgccgctt gaattctccg ctgcgcaacc gcagaagttc g
//
LOCUS       DASUCJ010001118         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1479152, whole genome shotgun sequence.
ACCESSION   DASUCJ010001118 DASUCJ010000000
VERSION     DASUCJ010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479152"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1335
                     /locus_tag="VFU90_13150"
     CDS             244..1335
                     /locus_tag="VFU90_13150"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5480784.1"
                     /translation="MPHIELEIAPETNIRELVAQLPIAAQVMEAFGLGCSGCGVSRSE
                     TIEQGARAHGLRVEPIVAALKQARLSGFVPTIANEDRRPATRAPREFRGRAKIGHVVA
                     IMSGKGGVGKSLVTALIALGLRRRHLRVGILDADITGPSIPRLFGLHEPLVVEADPVA
                     KTPQGHPQPLMVPAVSRTAIEVVSSNLLTDKEDTAMIWRGPILSGVIRQFFEQVLWSD
                     LDFLLIDLPPGTSDAPLTVLQSLALDGVVLVTMPQALATMIVRKAANLVHQLKKPVLG
                     VVENMSYFVAPDTGKTYELFGPSYAGRVAELAQAPVLARIPIDPEKALLADRGAVEEI
                     DDPICDELAAKLLVAIEAHPKPKETISLV"
     gene            complement(1405..2622)
                     /locus_tag="VFU90_13155"
     CDS             complement(1405..2622)
                     /locus_tag="VFU90_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480785.1"
                     /translation="MSGHYVRALALASIAALAACSGGSTNQTLPSGVGSGTSPLAFHN
                     DVPNTQAACDYMPMPGQARCYALIETGIVPRKGIQPNVSGYGPSDYVSAYKLPHGTLS
                     GVGQKIGIVDAYDDPNAESDLQVYRSQFGLPTCTTANGCFKKVNQVGQQGNYPKSDRG
                     WAAEESLDVDMASAICPHCKIILVEANSNYFTDLGASVDEAVKLKANVVSNSYGGGEG
                     YSSDPDYDHPGHVITASSGDSGYGAQQPCSLATVVCVGGTTLSKGGGGTRGWTEQSWN
                     HAGSGCSAYVTKPSWQTDSGCTMRSEADTSSVAVGAAVYDTFGNGGWIVLDGTSVSSP
                     IVASEYALAKNAKSQNYAQGIWVAGGTSKFYDITSGPANGSCPSQYKYICTPGKGYDG
                     ITGWGTAHGVKGY"
     gene            complement(2774..>3995)
                     /locus_tag="VFU90_13160"
     CDS             complement(2774..>3995)
                     /locus_tag="VFU90_13160"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480786.1"
                     /translation="NDGGLVLGGQYDESRWDDGGTADARPLERSHPNARALLARIDGH
                     SCVVNRKTFDALALAPDTPGIERDAAGAPTGKLFLQANWRAQTLFLAQVPLAIRRAAE
                     RRAVEQALSRGALHLHAQLVGFERSAYAGEIDALRALPAKIYPKICEPDAVLARELGL
                     PYVGGDVFLDGSLGSRTAALFEPYADGDTCGALRFSDDELLAYYAQAEALGIAAGVHA
                     IGDAAIEQSIRVWERVLAGKPSARGCRHFIEHFELATPEQIDACARMNLFLSMQPQFD
                     ASWAQRGGMYETRLGETRKRSMNALARIRAAGATVCGGSDSPVCALDPLAGMQACVEH
                     HEPSERLAPDAALFAYTVAAARLGYAEGATGNLLPGLAADFVVLDADPLEGTPFASCR
                     VRQTWSDGSLVWAA"
BASE COUNT          673 a         1316 c         1308 g          698 t
ORIGIN      
        1 agcgcgccgc gatgcgcggc cgtgtcgatg cccagcgcca gccccgcaat caccgggcgg
       61 ccgagtcgcg ccgccagcgc gaacgcgaat tccgccgcct cgggcggcgg cgtccggctt
      121 ccgacgatcg cgacgccgcc ggatgggatc gtgccgcgcg tatacaaacg tcgcatgcaa
      181 acccggccgt tcgccgacag aattgggtgg acccacagcc tggactccgg gaagaggaac
      241 cgcgtgccgc acatcgagct cgagatcgcg cccgaaacca acattcgcga actggtcgcc
      301 cagctgccga ttgcggccca agtcatggaa gccttcgggc tcggctgcag cggctgcggc
      361 gtcagccgct ctgaaacgat cgagcaaggc gcgcgcgcgc acggactgcg cgtcgagccg
      421 atcgtcgccg cactcaaaca agcccggctt tccggcttcg tccctacgat cgccaacgaa
      481 gaccgtcgcc cggccacgcg ggcgccgcgc gagttccgcg gacgcgcgaa gatcggtcac
      541 gtcgtcgcga tcatgtcggg aaaaggcggc gtcggaaaat cgttggtgac ggcgctgatc
      601 gcgctcgggc tgcggcggcg ccatctgcgc gtcggtattc tcgacgccga catcacgggg
      661 ccgtcgattc cgcgcttgtt cggcttgcac gagccgttgg tggtagaagc ggatccggtt
      721 gccaagacgc cgcaaggcca tcctcagccg ctgatggttc cggcggtgtc gcgcacggcg
      781 atcgaagtgg tttcttcgaa tttgcttacg gataaggaag acaccgcgat gatctggcgc
      841 ggcccgattt tgagcggcgt gattcggcag tttttcgagc aggtgctgtg gagcgatctc
      901 gacttcttgc tcatcgattt gccgccgggc acttcggacg ctccgctgac cgtactgcag
      961 tcgctggcgc tcgacggcgt cgtgctggtg acgatgccgc aagcgctcgc gacgatgatc
     1021 gttcgtaagg ctgccaatct ggttcaccag ttgaagaagc cggttctcgg cgtcgtcgag
     1081 aacatgtcgt acttcgtcgc gcccgacacc ggcaagacgt acgaactctt cggtccgtcg
     1141 tacgccggcc gcgtggccga gttggcgcag gcgccggtgc tcgcgcgcat cccgatcgat
     1201 ccagaaaaag cgctgctggc cgatcgcggc gccgtggaag aaatcgacga tccgatctgc
     1261 gacgagctcg cagccaaact gctggtcgcg atcgaagcgc atccgaaacc caaggaaacg
     1321 atctcgctcg tctagcgcgc aaacgaaaaa acgggcgcgg caatcgtgcc gcgcccgttc
     1381 gtcgttgcgt cgccgcgtcg agagctagta gccctttacg ccgtgggcgg tgccccaacc
     1441 ggtgatgccg tcgtagccct tacccggcgt gcagatgtat ttatactgcg acgggcagga
     1501 gccattggcg ggcccgctgg tgatgtcgta gaacttgctc gtgccgccgg caacccagat
     1561 gccctgcgcg tagttctgcg acttggcgtt cttggccagc gcgtattcgc tggcaacgat
     1621 cggcgacgag acgctcgtgc cgtcgagcac gatccagccg ccgttaccga acgtgtcgta
     1681 caccgcggcg ccgaccgcca ccgacgacgt gtcggcttcg gagcgcatcg tgcagcccga
     1741 gtcggtctgc cacgacggct tcgtcacgta agcgctgcaa ccgctgccgg cgtgattcca
     1801 cgactgttcg gtccagccac gggttccgcc gccgcccttc gacagggtcg ttccgccgac
     1861 gcagacgacc gttgcgagcg agcagggctg ctgcgcgccg taaccgctgt cgcccgagct
     1921 ggccgtgatg acgtgtccgg gatggtcgta gtccggatcg gagctgtagc cctcgccacc
     1981 gccgtaggag ttgctcacta cgttcgcctt gagtttgacc gcttcgtcga ccgaagcacc
     2041 caaatcggta aagtagttag agttcgcttc gaccaggatg attttgcaat gcgggcagat
     2101 cgccgacgcc atgtcgacgt cgagcgactc ttccgccgcc caaccgcggt cggacttcgg
     2161 atagttaccc tgctggccta cttggttcac ttttttgaag caaccgtttg ccgtcgtgca
     2221 agtcggcagc ccgaactgcg aacgatacac ttgaaggtcg gattcggcgt tgggatcgtc
     2281 gtaggcatcg acgatgccga tcttctgtcc cacgccgctc agcgtgccgt ggggcagttt
     2341 ataggccgac acgtaatccg acggcccgta accgctaacg ttgggctgga tgcccttacg
     2401 cggcacgatg ccggtttcga tcagcgcgta gcagcgcgcc tgaccgggca tcggcatgta
     2461 gtcgcaggcc gcctgcgtat tgggaacgtc gttgtggaag gcgagcggcg acgttccgct
     2521 tcctactcca ctgggtaacg tctgattcgt cgaaccgccg gaacaggcgg ccaaagcagc
     2581 aatgctcgca agtgcgagtg ctcgaacgta gtgtccgctc aaggcaacag cctcctcaaa
     2641 ggaaaaaatg tcaagggggt cgccctaatg tagcactcag gccaccctca tagtggagat
     2701 gatttgcacc taggtgcgct cttcacctat tcgcggccga aaagccgcgc attgcaaggc
     2761 tggcaagccg tcgctatgcc gcccaaacga ggctgccgtc gctccaggtc tggcgcaccc
     2821 ggcagcttgc gaagggcgtt ccctcgagcg gatccgcgtc gagcaccacg aaatcggcgg
     2881 ctaagccggg caaaagattc ccggtcgcgc cttcggcgta accgagccgc gccgcagcaa
     2941 cggtgtaggc gaacagcgcc gcgtccggtg ccaagcgttc ggacggctcg tgatgctcga
     3001 cgcaggcctg catcccggcc aacggatcga gggcgcagac ggggctgtcg gagccgccgc
     3061 atacggttgc tccggcggcg cgaatgcgcg cgagcgcgtt catcgaacgt ttgcgcgtct
     3121 ccccgagacg cgtttcgtac atcccgccgc gctgcgccca cgacgcgtcg aactgcggct
     3181 gcatcgacaa gaagaggttc atgcgcgcgc acgcatcgat ctgctcgggc gttgccagct
     3241 cgaagtgttc gatgaagtga cggcagccgc gcgccgacgg cttgccggcg aggacgcgtt
     3301 cccaaacgcg gatgctctgt tcgatcgccg cgtcgccgat ggcgtgcacg ccggcggcga
     3361 tccccagcgc ttcggcctgc gcgtagtatg ccagcagctc gtcgtcgcta aagcgcagcg
     3421 cgccgcacgt atcgccgtcg gcatagggtt cgaacagcgc ggccgttcgg cttccgagcg
     3481 aaccgtcgag gaagacgtcg ccgccgacgt acggcagtcc gagctcgcgc gccagtaccg
     3541 cgtcgggttc gcagatcttc ggatagattt tggccggcag cgcgcgcagc gcgtcgatct
     3601 cgccggcgta cgccgaacgc tcgaagccca ccaactgcgc gtggaggtgg agagcgcccc
     3661 gcgacaacgc ctgctcgacc gcacgtctct cggcggcgcg ccggatcgcg agtggaacct
     3721 gcgcgaggaa tagcgtttgg gcgcgccagt tggcttgcag aaagagcttg cccgtcggtg
     3781 cgccggcggc gtcccgctcg atcccgggcg tatccggtgc gagtgcgagc gcgtcaaacg
     3841 tcttgcggtt gacgacgcat gagtgcccgt cgatccgcgc gagcaaagcg cgcgcgttcg
     3901 ggtgcgagcg ctccagcggc cgcgcgtcgg ccgtgccgcc gtcgtcccag cggctttcat
     3961 cgtactgccc gccgagcacg agcccgccgt cgttc
//
LOCUS       DASUCJ010001119         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_577793, whole genome shotgun sequence.
ACCESSION   DASUCJ010001119 DASUCJ010000000
VERSION     DASUCJ010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577793"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFU90_13165"
     CDS             <1..322
                     /locus_tag="VFU90_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480787.1"
                     /translation="LDSSQPFALADALRAVRERIESLPDNVFVDPDVRRAPEAAVAHA
                     LATLQKRGTLTGNGGRYTLTGDRTDARFPHVADMVAFQRNMLDETLASARRLGATPAT
                     AELG"
     gene            complement(314..>1504)
                     /gene="hpnJ"
                     /locus_tag="VFU90_13170"
     CDS             complement(314..>1504)
                     /gene="hpnJ"
                     /locus_tag="VFU90_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEU5480788.1"
                     /translation="ARRLKAQNPNVKVGFIGAHVAVLPEETLRENEIIDFVCRNEFDY
                     TCKELAEGRPWNEILGLTYRDANGELKRTEERPQIHDWDAMPSVLPIYAKFLDVTKYY
                     NGYLLHPYVSWYTGRGCTAKCTFCLWPQTIGGHVFRHKSPEAVGRDLDEAKAIFGSTV
                     AEYMFDDDTLTNEKAYAIELSKHMKRLKLSWACNARAHVDYDTLKILRDNGLRVLLVG
                     FESGNQEILYRIKKGLKLEMARKFMKDCHKLGIKVHGTFIIGLPVDTPETVRETIEFA
                     KELDPHTIQVSIASPYPGTELYNQALANGWFARDDLVSGKGIQTSTLRYDTLSTAEIE
                     DSVERMYREFYFRPKKIAKIVGEMVTSRQMLVRRLREGGEFFSYLRARRVQAGERRLE
                     AAST"
BASE COUNT          256 a          481 c          451 g          316 t
ORIGIN      
        1 gctcgattcc tcgcagccgt tcgcgctcgc cgatgcgctc cgcgcggtgc gcgagcggat
       61 cgaatcgctg ccggataacg tgttcgtcga tcccgacgtg cgccgcgccc cggaggcggc
      121 cgtcgcccac gcgcttgcca cgctgcaaaa acgcggcacg ctgaccggaa acggcgggcg
      181 ctacacgctc accggcgacc gaaccgatgc gcgcttcccc cacgtcgccg acatggtcgc
      241 attccaacgc aacatgctcg acgaaaccct cgcgtcggcc cgccggctgg gtgcgacgcc
      301 cgctaccgcg gagttaggtt gacgccgctt cgagccggcg ttcgccggcc tgtactcgcc
      361 gggcccgcaa atagctgaag aactcgccgc cttcgcgtag gcggcggacc agcatttgac
      421 ggctcgtgac catctcgccg acgatcttgg cgatcttttt cggccggaag tagaactcgc
      481 gatacatccg ctcgaccgaa tcttcgatct cggccgtcga gagcgtgtcg tagcgcaacg
      541 tcgacgtctg aattcctttt ccggagacta agtcgtcgcg cgcgaaccag ccgtttgcga
      601 gagcttgatt gtagagttcc gttcccggat acggcgacgc gatcgaaacc tgaatcgtgt
      661 gcgggtcgag ctccttcgcg aattcgatcg tttcgcgaac cgtctcggga gtgtcgaccg
      721 gcaggccgat gatgaacgtg ccgtggacct tgatgccgag cttgtggcaa tccttcatga
      781 atttgcgcgc catctcgagc ttcagaccct ttttgatgcg gtacaggatc tcttgattgc
      841 cggattcgaa gccgaccagc agcacgcgca gcccgttgtc gcgcaggatc ttgagcgtat
      901 cgtagtcgac gtgcgcgcgg gcgttgcaag cccagctgag cttcaaacgc ttcatgtgct
      961 tgctgagctc gatcgcgtac gctttttcgt tggtcagagt atcgtcgtcg aacatgtatt
     1021 ccgccaccgt cgaaccgaag atcgctttcg cttcgtcgag gtcgcggccg actgcctcgg
     1081 ggctcttgtg gcggaagacg tggcccccga tcgtttgcgg ccatagacag aacgtacact
     1141 tggccgtgca gccccggccc gtgtaccaag agacgtacgg gtgcagcagg tagccgttat
     1201 agtatttcgt gacgtccaga aacttggcgt agatcggaag gacgctcggc atcgcatccc
     1261 agtcgtggat ttgcggccgc tcttcggtac gtttcaactc gccgttcgcg tcgcgatacg
     1321 tcagcccgag aatctcgttc cacggacgcc cttcggccag ttccttgcac gtatagtcga
     1381 actcgttccg gcagacgaaa tcgatgatct cgttctcgcg cagcgtttct tccggaagca
     1441 ccgccacgtg cgcgccgatg aacccgacct tgacgttggg attttgcgct ttgagccggc
     1501 gcgc
//
LOCUS       DASUCJ010001120         1429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_623087, whole genome shotgun sequence.
ACCESSION   DASUCJ010001120 DASUCJ010000000
VERSION     DASUCJ010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623087"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="carA"
                     /locus_tag="VFU90_13175"
     CDS             complement(<1..1085)
                     /gene="carA"
                     /locus_tag="VFU90_13175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5480789.1"
                     /translation="MEASPPAALFLADGSRFDGRGLGFRGTALGEAVFFTGMTGYEEA
                     LTDPSYAGQILTFTYPMIGNYGVSGSAAQYSRACVAGTVMKQIAEHPSHHLSRGSLRS
                     WLDEQRVPAMVGADTRAITIALREHGTIWAALAVGEEALDAAERALAEFVRTATTQGL
                     VAQVATPAPFVEEGTPGGARVTLLDCGVKRAILRDLHALGARVTVLPYDAAADQILAG
                     DPEAVLISPGPGDPTDLPGTIGVLRELIGRKPLFGVCLGHQLLALACGARTYKLPYGH
                     RGGNQPVKDELAGDVIITAHNHGYAVDARSLPADLEATMTNLNDGTNEGFRHRTLPIA
                     AVQFHPEASPGPSDARRIFAQWFASAGL"
     gene            complement(1130..>1429)
                     /locus_tag="VFU90_13180"
     CDS             complement(1130..>1429)
                     /locus_tag="VFU90_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480790.1"
                     /translation="AVGFSGLEIAVGAYAAALPGLPLLRFVELLSTNPARILGVTGGT
                     LAIGSPADVSVFADRPWQVDPSTFASKGRVTPFAGRTLPRAIVATIVGGEVAFAR"
BASE COUNT          248 a          498 c          458 g          225 t
ORIGIN      
        1 agaccggccg aagcgaacca ctgcgcgaaa atccggcgtg cgtcggatgg ccccggcgac
       61 gcctcggggt gaaattgaac ggccgcgatc ggcagcgtcc gatgccggaa tccttcgttg
      121 gtaccgtcgt tgagattggt catcgtcgct tccaagtcgg ccggcagcga gcgtgcgtcg
      181 accgcgtacc catggttgtg cgcggtgatg atgacgtcgc cggccaactc gtccttaacc
      241 ggctggtttc caccgcggtg accgtacggc agcttgtacg tgcgcgcgcc gcacgccagc
      301 gccagcagct gatgccccag acatacgccg aacagcggct tgcgcccgat cagttcgcgc
      361 agcacgccga tcgtaccggg aagatccgtc ggatcgccgg gacccggcga aatgagcacc
      421 gcctcgggat cgccggccag aatctggtcg gccgccgcgt cgtagggcag cacggtaacg
      481 cgcgcgccga gcgcgtgcag atcgcgcaag atcgcacgct tgacgccgca atccagcagc
      541 gtgacgcgcg ctccgccggg cgttccttct tcgacgaacg gcgccggcgt cgcaacttgc
      601 gccaccaatc cctgggtcgt cgccgtgcgt acgaactccg cgagcgcccg ttccgccgca
      661 tcgagcgctt cttcaccgac cgccagcgcc gcccaaatcg ttccgtgctc gcggagcgcg
      721 atcgtgattg cccgcgtgtc ggcgccgacc attgccggaa cgcgctgttc gtcgagccag
      781 cttctcaagc tcccgcgcga gagatggtgc gacggatgct cggcgatttg tttcatgacg
      841 gttccggcca cgcacgctcg cgaatactgt gcggcggatc ccgagacgcc gtagttcccg
      901 atcatcggat aggtgaacgt taggatttgc ccggcgtacg acggatccgt cagcgcttct
      961 tcgtacccgg tcattccggt aaaaaagacg gcttcgccca gcgccgttcc acgaaaaccg
     1021 agtccccggc cgtcgaaacg gcttccatcg gcaaggaaaa gtgcggcggg cggggaggcc
     1081 tccatcgggt ggcttgcgtt agcgaaccgg acgccggtcc cctcgcgcgt cagcgagcaa
     1141 acgcaacctc gccgccgact atcgttgcga cgatcgcgcg cggcagcgta cgcccggcga
     1201 acggcgttac ccggcctttc gaagcgaacg tcgacggatc gacctgccac ggccggtcgg
     1261 cgaacacgct gacgtcggcc ggcgatccaa tcgccagcgt tccaccggtg acgccgagga
     1321 tgcgcgccgg attggtcgac aacagctcga cgaaacgcag cagcggcagc cccggcaacg
     1381 cagctgcata cgcgccgacg gcgatttcca agccgctgaa tcccaccgc
//
LOCUS       DASUCJ010001121         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1112682, whole genome shotgun sequence.
ACCESSION   DASUCJ010001121 DASUCJ010000000
VERSION     DASUCJ010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112682"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..916
                     /locus_tag="VFU90_13185"
     CDS             74..916
                     /locus_tag="VFU90_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5480791.1"
                     /translation="MPANERQAEAWNGPESAHFVANADRYDRQLEPFTRALLERAGHG
                     TDHVVLDVGCGAGTTTLAAAAGAEHVVGVDLSQPLVDVARSRARVAKISNAEFVIADA
                     QTHDFVAGTFDLVISQFGLMFFDDPVRAFTNIRRALTYGGRAVFVCWQELLANEWLSL
                     IADAVRRHAELPEFGGQIRGPGMFALCKPDEIATLLGAAGFDQVECDSCTPAMLIGGG
                     GSFDDSVDFLLSAGMPRGLLSHADESDRDDVLRIVRAELADRYEEGVGIRLRAAAWVV
                     AAEA"
BASE COUNT          201 a          363 c          387 g          189 t
ORIGIN      
        1 gtatcttgcg accttgatcg gctgatatgg cgccgtgcga tgatggggca cggcgaacga
       61 cggcgaggca ccaatgccgg caaatgaacg acaagcagag gcatggaacg gacccgagtc
      121 cgcgcacttc gtcgccaacg ccgaccgata cgaccgtcaa ctcgaaccct ttacgcgggc
      181 gcttctggag cgcgccgggc acggcaccga ccacgtcgtg ctcgatgtcg gctgcggggc
      241 gggcacgacg acacttgccg cggccgccgg cgccgaacac gtcgtcggcg tcgacttgtc
      301 gcagccgctc gtcgacgtgg cacgaagccg agcacgcgtt gcgaaaatca gcaacgcgga
      361 gttcgtcatc gctgacgcgc agacacacga cttcgtcgcg ggcacgttcg atctcgtgat
      421 cagccagttc ggactgatgt tcttcgacga tcccgtgcgc gctttcacca acatccgacg
      481 ggcgctcacc tatggcggcc gtgccgtgtt cgtttgttgg caggagcttc tcgccaacga
      541 gtggctgtcg ctgatcgccg acgcagtccg ccggcacgcc gagctccccg agttcggagg
      601 acagatccgg ggaccgggca tgttcgcgtt gtgcaaaccg gatgagatcg cgacgctgct
      661 cggcgccgcc gggttcgatc aagtggagtg cgactcctgc acgcccgcga tgctcatcgg
      721 aggcggcggc agtttcgacg actcagtcga ctttctcctc agtgcaggca tgccgcgcgg
      781 tctgctcagt cacgcggacg aaagcgaccg cgatgacgta ctccggatcg tgcgagccga
      841 actcgccgat cgatacgaag aaggggtagg gatccgcctt cgagcggcgg cgtgggtggt
      901 ggccgccgag gcatagccgc tggcatggca ccggatgaga gacggcaggc ttcgtagacg
      961 gcggtatgcc ggcgacgaac gcgtcgatcg gtaggccgcc tgagcgcttg tctctgccgc
     1021 gctcatgccc ttcggctcta caacgacatc gctcgacggg cgcacgttcg tgaggtacgg
     1081 gggacgacct cgcgtggagg gaggcgagcg tcatgactgc gacgatcgaa aagccgcccc
//
LOCUS       DASUCJ010001122         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_990180, whole genome shotgun sequence.
ACCESSION   DASUCJ010001122 DASUCJ010000000
VERSION     DASUCJ010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990180"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="hemA"
                     /locus_tag="VFU90_13190"
     CDS             complement(<1..897)
                     /gene="hemA"
                     /locus_tag="VFU90_13190"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU5480792.1"
                     /translation="MPIVCVGLSHHTAPVDVRERHAFPASRMAEALVALRDYPAVREA
                     AMLQTCGRLEIYGALDDYEAGVAQIKSFLTNFRHGTTGYDIESFLYTLLGRQAVEHLF
                     RVSTGLDSMLIGEPEILGQVKDAYVQAQHAQSLGKVLHRLFREALVAGKTARAQTSIG
                     DESSSVATAAVEAAKARFQTLDGKTVVVIGAGKMGRTAVKRLRLAGAAHVIVTNRTIA
                     RARELALESGFGEAAELPRLPEALAVADVVVASTGASHFILTPERVAEAMAQRPERPL
                     FVIDIAVPRDADPAVAEIPNVTL"
     gene            complement(897..1598)
                     /locus_tag="VFU90_13195"
     CDS             complement(897..1598)
                     /locus_tag="VFU90_13195"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5480793.1"
                     /translation="MIDPTLRGRASGEPRSAPLTSLVEVSIPRLYAYHRALRQAQTDG
                     KTTITSHGLAALLGHTIASTQVRKDLSALGPLGRRGQGYAIDPLVEQLAWVLGLEHDW
                     RIAIVGFGYLGHAFASFITAREARFRVAAIFDRSPAVIGQVWQDIVVHDVAEIDRVLR
                     ESGCNIGVIAVPADEARPAAQRIAALGVRAILNFAPIALDLRAPVIVRNIDLAGEMSI
                     LTHRLTLDLLGDASV"
     gene            complement(1595..>2065)
                     /locus_tag="VFU90_13200"
     CDS             complement(1595..>2065)
                     /locus_tag="VFU90_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5480794.1"
                     /translation="AFGGRADVMAYLSPDGPVYQAGTLSGNPIAMAAGIATLKAVRDD
                     ATLYGRLDVLARLLVEGLHEVMSKHGIPHYSTQAGSMFSTFFAEGPVTDLESALKSDT
                     DSFAKYFGAMLDRGIYLAPSQFEAGFLSTAHTTRHVEQTLAAADAALAYLLAAT"
BASE COUNT          357 a          672 c          698 g          338 t
ORIGIN      
        1 cagcgtaacg ttggggatct ccgcgaccgc gggatccgcg tcccgcggaa ccgcaatgtc
       61 gatgacgaac agcggacgct cgggccgttg cgccatcgct tccgcgacgc gctcgggcgt
      121 aaggatgaag tgcgacgcgc cggtcgaagc gacgaccacg tcggcaaccg cgagcgcttc
      181 cggtaagcgc ggcagttcgg cggcttcgcc aaaacccgat tcgagcgcca gctcgcgcgc
      241 gcgtgcgatc gtgcggttcg tcacgatgac gtgcgccgcg ccggcgagcc gcagccgttt
      301 taccgcggtc cgtcccatct tgccggcgcc gatcacgacg acggtttttc cgtccagcgt
      361 ttggaatcgc gctttggcgg cttcgacggc ggcggtggcg accgaactgg actcgtctcc
      421 gatgctcgtt tgcgcgcgcg cggtcttgcc ggcgaccaaa gcctcgcgga acagacggtg
      481 gagcaccttg cccagcgatt gtgcgtgctg ggcctgcacg tacgcgtcct tgacttgacc
      541 gaggatttcc ggttcgccga tcagcatcga atcgagcccg gtagataccc ggaagaggtg
      601 ctcgacggct tgccggccga gcagcgtgta cagaaacgat tcgatatcgt aaccggtcgt
      661 gccgtgccgg aagttcgtga gaaaagactt gatctgcgcg acgccggctt cgtagtcgtc
      721 gagcgcgccg tagatttcca agcgcccgca cgtctgcagc atcgcggctt cccgcaccgc
      781 cggatagtcg cgcagcgcga ccagcgcttc ggccatccgc gaggccggaa acgcgtgccg
      841 ttcgcgcacg tcgaccggcg ccgtgtggtg cgacaaaccg acgcaaacga tcggcactag
      901 acgctcgcgt ccccgagaag gtcgagggtg agccggtgcg tgagaatcga catctcgccg
      961 gcgagatcga tattccggac gatcaccggc gcccgcaggt cgagcgcgat cggtgcgaaa
     1021 ttcaaaatcg cgcgcacccc cagcgccgca atccgctggg ccgccggccg cgcttcgtcg
     1081 gccggaacgg cgatgacgcc gatgttgcag ccgctctcgc gcagtacgcg atcgatctcc
     1141 gcgacgtcgt ggacgacgat gtcttgccag acttggccga tcacggccgg cgaccggtcg
     1201 aagatggcgg cgacgcgaaa tcgcgcctcg cgggcggtga tgaacgaagc gaacgcatgg
     1261 ccgaggtagc cgaagccgac gatcgcaatc cgccagtcat gttccaatcc tagaacccac
     1321 gcgagttgtt cgactagcgg atcgatcgca tatccttggc cgcgacgtcc taggggaccc
     1381 aaagccgaga ggtccttgcg gacctgcgtg ctggcgatcg tgtgcccgag cagcgccgcc
     1441 agcccgtggg acgtgatcgt cgttttcccg tcggtttgcg cttggcggag ggcgcggtga
     1501 taggcgtaca gccgcgggat cgagacctcg accagtgagg tcagcggagc ggagcgaggc
     1561 tcgccggaag cccgtccgcg cagggtgggg tcgatcacgt tgccgccaag aggtaggcga
     1621 gcgccgcgtc ggccgccgcc agcgtttgct cgacgtgacg cgtcgtgtgc gccgtcgaaa
     1681 ggaacccggc ctcgaactgc gagggcgcga gataaatgcc gcgatcgagc attgcgccga
     1741 aatacttcgc gaacgaatcc gtatcggact tcaacgcact ttctaaatcg gtcaccgggc
     1801 cttcggcgaa gaacgtcgag aacatcgacc cggcttgcgt cgagtagtgc gggatgccgt
     1861 gcttgctcat cacttcgtgc aagccctcga ccagcaggcg cgcgagcacg tcgaggcgcc
     1921 cgtacagggt cgcgtcgtcg cggactgctt tgagcgtcgc gatgccggcc gccatcgcaa
     1981 tcgggttgcc cgacaacgtg cccgcttgat agaccgggcc gtcgggggac aggtacgcca
     2041 tcacgtcggc gcgtccgccg aacgc
//
LOCUS       DASUCJ010001123         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_959378, whole genome shotgun sequence.
ACCESSION   DASUCJ010001123 DASUCJ010000000
VERSION     DASUCJ010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959378"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFU90_13205"
     CDS             <1..373
                     /locus_tag="VFU90_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480795.1"
                     /translation="AGKIYDVSFFFGGLDQDGIWQMVDLPALPHPDAYYSVYVKQLAD
                     FKEGERTVTFTNPAYWASHASQQEFNLSKTSPRDLLHLQGQKPDPRYAKIVDDFRSFG
                     PDEFRRNVAAAQQRARTSACK"
     gene            417..1190
                     /locus_tag="VFU90_13210"
     CDS             417..1190
                     /locus_tag="VFU90_13210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5480796.1"
                     /translation="MLAVFGTAAALTYAAYEFAESPGNQLFGKTVVSGPKNEKVVALT
                     YDDGPNPPYTGQILSVLRKERVHATFFVVGRAVEAYPAVVRAEVADGDAIGNHTWSHG
                     HLVLYDPGSLKQTLQRTDRAIYEAAGTHTRIMRPPYGGRDWFVLDEVRKLGYTPVMWS
                     VPLPNDWEYPPAPVIASRVLRYVGDGSIIDLHDGNRGIVCSARSKVAAHVCDRSADVE
                     ATRLIIESLKRMGYRFVTIPELLEMGRGAAAHVGSGAGG"
     gene            1595..2167
                     /locus_tag="VFU90_13215"
     CDS             1595..2167
                     /locus_tag="VFU90_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5480797.1"
                     /translation="MRTIRSDRLQLVPVTIENAGVLWEVLQKPDLRDFQDLPDLGVEQ
                     FKRSVAERPAALDPAAAGRFEWLVYSNPPAGEPLGWVSLRISDRMLTTAEIGYSIVRG
                     HRNRGIATEAVGMLIEEGFTRTRLRVVRAYCVPENAPSRAVLRRHGFEDEGILPHGAT
                     VQGQPVDVIAHSLARERWESRLTENLPSES"
     gene            complement(2139..2627)
                     /locus_tag="VFU90_13220"
     CDS             complement(2139..2627)
                     /locus_tag="VFU90_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5480798.1"
                     /translation="MTHVHLATGLAIRGTGSGRAVLLVASSYPDHPDPLWNLPGGRQR
                     PGELLAETVAREIREETGLPARVEKLAYVSESYDGDVHFVNATFHVTIEDGNLEPRQP
                     PARGGDHVVAAAWIGVEDIASRIAVAVVRDPLVAYLHDELPARYAGFLEAGITIRWED
                     SP"
     gene            complement(2624..>3532)
                     /locus_tag="VFU90_13225"
     CDS             complement(2624..>3532)
                     /locus_tag="VFU90_13225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5480799.1"
                     /translation="GVLAALALHAPRVVFHLHDHRPFCPNGDRLYPQGGGICGAPMGV
                     ATCGWHALRNGCAYGPRKRTLDLIRIREGVARAVAMADATVTLSRYVADLAERNGVPA
                     DRTRVVAPPLPLEAFAPNPAPPRGGAGVLFAGRVMPSKGGRSLVRALARIPNPQRPVL
                     RIAGEGPDLPGVLAAAGRQGVRVDALGRLAASELRAAYDDAAVLAMPSLWGEPFGLVG
                     IEAFARGRPVVAYDAGAIAEWLAPGAGRVVPRGDEASFATAIQELLEPGAWAKASALA
                     FAAARAYAPAQHVERIESIYAGAARP"
BASE COUNT          616 a         1168 c         1197 g          551 t
ORIGIN      
        1 ggcggggaag atctacgacg tgagcttttt cttcggcggc ctcgaccagg acggcatctg
       61 gcagatggta gatttgccgg ccttacccca tccggacgcc tactattcgg tttacgtcaa
      121 gcaactcgcc gacttcaagg aaggcgaacg cacggtgacg tttacgaatc ctgcctactg
      181 ggcaagccac gcttcgcagc aggagttcaa cctctccaag acgtcaccgc gcgacctact
      241 gcacttgcag ggtcaaaaac ccgaccctcg ctacgcaaaa atcgtcgacg atttcagatc
      301 gttcgggccg gatgaattcc gtagaaacgt tgcggcggct cagcagcgcg cccgcacgag
      361 cgcctgcaag taacgtggcc gaaccgacga tcgcgggacg gcggtcggcc gtccgaatgc
      421 tggcggtctt cgggaccgcc gccgcgctga cgtacgccgc ctacgagttt gcggaaagtc
      481 cgggcaatca gctcttcggg aagacggtcg tgagcggccc aaaaaacgag aaggtcgtcg
      541 cgctcacata cgatgacgga cccaacccgc cgtataccgg ccagatattg tcggtcctgc
      601 gcaaggaacg cgttcacgcg accttcttcg tcgtgggacg cgccgttgaa gcgtacccgg
      661 cggtcgtgcg tgcggaagtg gccgacggag acgcgatcgg aaaccatacc tggagccacg
      721 gacatctcgt tctctacgat cccggcagtc tgaaacaaac gctgcagcgg acggatcggg
      781 cgatctatga agcggcgggg acccacacgc ggatcatgcg gccgccctac gggggccgcg
      841 actggttcgt gctcgacgaa gtccgcaaac tcggctacac gccggtgatg tggtccgtgc
      901 cgctgccgaa cgactgggag tatccgcccg cgcccgtgat cgcttcgcgg gttctccgct
      961 acgtcggcga cggttcgatc atcgatctgc acgacggaaa ccgcggcatc gtgtgctcgg
     1021 cgcgctcgaa ggttgccgcg cacgtctgcg accgttccgc cgacgtcgaa gcgacgcgct
     1081 taatcatcga gtcgctcaag cggatgggtt atcgcttcgt gacgataccg gagctgctcg
     1141 agatgggccg cggcgccgcc gcgcacgtcg gcagcggcgc gggaggctaa cgctaggcgt
     1201 agagacgtcc gtgcagccgg atgaagcctc ccgggtgacg ttggcgaggg tcgtgatggg
     1261 tccagtggac gaccggcggg cggccgcggt cgtgaaccat ttcgccgcag acttcaatgc
     1321 gatcgcccgt tctcaccggg cagcgcgggg cgatgtctac gttgtcgacg atctcaaccg
     1381 gcccggacgc gctgcgtgcg aggaacgttt cgtgcatgca atgagtgcgc gacccgacaa
     1441 agaagcgagg tggggacgct acggtagctt cgaagtcgac ccgtgccggt gccgagccgg
     1501 catacgctgc ggaaaggttc atcgcgcacg tcgttcacgc ggcggcgacg cgatgacaag
     1561 aacgggacgc tcggaccgcg aaagggcgcc gcgcatgaga acgattcgca gcgatcgtct
     1621 gcagctggtg ccggtaacta tcgagaacgc cggcgtgctc tgggaagtgc tgcaaaaacc
     1681 ggatctgcgc gattttcagg acctccccga tctcggcgta gagcagttca aacgctcggt
     1741 tgcggaacgg ccggccgcgc tggatccggc cgccgcgggc cgcttcgagt ggctggttta
     1801 ctcgaacccg cccgccggcg agccgctggg ctgggtttca ctgcgaatat ccgatcgcat
     1861 gctcaccacc gcggaaatcg gctacagcat cgtccggggg catcgtaacc gcggcatcgc
     1921 caccgaggcg gtcggcatgc tgatcgaaga agggtttacg cgcactcgtc tgcgcgtcgt
     1981 acgcgcctac tgcgtgccgg aaaacgctcc gtcgcgcgcg gtgctgcgcc gccacggctt
     2041 cgaagatgaa ggcatcttac cccacggtgc caccgtgcag ggtcagccgg tggacgtgat
     2101 cgcgcactcg ctggcgcgcg aacgctggga atcgcgcctc acggagaatc ttcccagcga
     2161 atcgtaatcc ccgcttcgag aaagcccgca tagcgggcgg gcagctcgtc gtgcaagtag
     2221 gctaccaacg gatcgcggac gaccgcgacc gcgatgcgcg aggcaatgtc ttcgacccct
     2281 atccaagccg ctgcaacgac gtgatcgccg cctcgcgccg gcggctgcct cggttcgagg
     2341 ttgccgtcct cgatcgtgac gtggaacgtc gcgttgacga aatgcacgtc gccgtcgtag
     2401 ctctcgctta cgtacgcgag cttttcgacg cgcgccggca agccggtttc ttcacgaatc
     2461 tcgcgtgcaa cggtttcggc cagcagctcg ccgggccgct gccgcccgcc ggggaggttc
     2521 cacaacggat ccgggtggtc cggatagctg gacgcgacca gcagtacggc gcgaccgctg
     2581 ccggttccac ggatcgccaa gccggttgcg aggtgaacgt gcgtcacggg cgggcggctc
     2641 cggcgtagat cgactcgatg cgttcgacgt gctgcgccgg tgcgtaggcg cgcgccgccg
     2701 cgaacgccag cgccgacgcc ttcgcccacg cgccgggttc gagtaactcc tggatcgccg
     2761 tcgcgaatga ggcttcgtcc ccgcgcggca cgacgcgtcc ggccccgggc gccagccact
     2821 cggcgatcgc tcccgcgtcg taggcgacga ccgggcggcc gcgcgcgaac gcctcgattc
     2881 cgaccagccc gaacggctcg ccccagagcg acggcatcgc gaggacggcg gcgtcgtcgt
     2941 acgcggcccg cagctccgaa gcagccagac gcccgagcgc gtcgacgcgc acgccctgcc
     3001 ggccggccgc ggccaacact ccgggaagat cgggaccttc acccgcgatt cgcagtacgg
     3061 gacgctgcgg gttggggatg cgtgcgagcg ctcgaaccag cgagcggccg cccttggagg
     3121 gcattacccg gccggcgaac agcacgccgg cgccgcctcg tggcggcgcg ggattcgggg
     3181 cgaacgcttc cagcggaagc ggcggcgcca cgacgcgcgt tcgatcggcg ggaacgccgt
     3241 tgcgttcggc caggtccgca acgtaacgcg agagcgtaac ggtggcgtcg gccatggcga
     3301 cggcgcgggc cactccttcg cgaatgcgta tcagatcgag cgtgcgctta cgcggtccgt
     3361 acgcacagcc gttgcggagc gcgtgccagc cgcaggttgc cacccccatc ggagcgccgc
     3421 aaatgccgcc gccttgcgga tagaggcgat cgccgttggg acaaaacggc cgatgatcgt
     3481 ggaggtgaaa gaccacacgc ggcgcgtgca gtgcgagcgc cgccaggacg cc
//
LOCUS       DASUCJ010001124         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_500573, whole genome shotgun sequence.
ACCESSION   DASUCJ010001124 DASUCJ010000000
VERSION     DASUCJ010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500573"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFU90_13230"
     CDS             <1..532
                     /locus_tag="VFU90_13230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5480800.1"
                     /translation="QEGYIAGVGRKKEQRLVIAPLTQEDARELFEIIGSIEGLAARGA
                     AEAPDHARDSLVTQLRHINVKLSEASRPGHPDPLRIFDMDMSFHRHYVDAGAGPRLRA
                     LHDAIKPQAERYVRLYTSALIDEIGRSVAEHDTIIERIASANADGAQRSVERNWRNAA
                     VRLRRVISTLGERGSW"
     gene            complement(498..>1812)
                     /locus_tag="VFU90_13235"
     CDS             complement(498..>1812)
                     /locus_tag="VFU90_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5480801.1"
                     /translation="AVMNFTATQIGEIGSTYVIGAVIGSLVFGHLTDRYGRRKLFFVT
                     LAVYLLGTLLTAFSWNLSSFLAFRFLTGAGIGGEYAAINSAIDELIPATYRGRADLIV
                     NGSYWIGAAIGSASTLVILDPAILPPTLGWRVGFGIGAALGLLILFLRKSIPESPRWL
                     MTHGSGHESHRIMDAIEARVERSLGRTLEVCTTEPLVIHPRRSFGFGLVARVMFKQYW
                     RRSVLCLALITSQAFLYNAVFFTYVLILTRFYDVPAPRTGIYLLPFALGNFLGPVLLG
                     HLFDTIGRKQMIAGTYAISAALLVLTGWLFAQGYLSATSQTALWSIIFFFASCAASSA
                     YLTVSEIFPLEIRALAIAFFYSLGTAAGGIVAPTLFGHLIGSGSRVQLFLGYVLAAVL
                     MFAAAIVELIYGVRAERTGLEELAAPLSALVYEPATSSRVRQASK"
BASE COUNT          378 a          622 c          566 g          246 t
ORIGIN      
        1 acaggaaggc tacatcgccg gcgttgggcg gaagaaagaa caacgcctcg tcatcgcgcc
       61 gctcacgcag gaagacgcgc gcgagttgtt cgaaatcatc ggctcgatcg aaggactcgc
      121 ggcgcgcggc gccgccgaag cgcccgacca cgcgcgcgac tcgttggtca cgcagctccg
      181 gcacatcaac gtcaagctct ccgaagcgtc gcgccccggg cacccggatc cgctgcgcat
      241 cttcgacatg gacatgtcgt tccaccggca ctacgtcgac gcgggcgccg ggccgcgcct
      301 gcgcgcgctg cacgacgcga tcaagccgca agccgaacgc tacgtgcgcc tctacaccag
      361 cgcgctgatc gacgagatcg gccggtcggt tgccgagcac gacacgatca tcgagcgcat
      421 cgcgtccgcg aacgccgatg gtgcccaacg atcggtcgag cgcaactggc gcaacgccgc
      481 ggtccgcctc cgccgcgtca tttcgacgct tggcgaacgc gggagctggt agccggctcg
      541 tacaccagcg ccgacaacgg cgccgccaat tcctccaatc ccgtccgctc cgcacggacg
      601 ccgtagatca gctccacgat ggccgcggcg aacatcagca cggccgcgag cacgtagccg
      661 aggaacaatt gcacgcgcga cccgctgccg atcagatgcc cgaacagcgt cggcgccacg
      721 atcccgcccg ccgccgtgcc caacgaatag aagaatgcga tcgccagcgc ccggatctcg
      781 agcgggaaaa tctcgctcac ggtgaggtag gcggagctgg cggcgcacga ggcaaaaaag
      841 aagatgatcg accagagcgc ggtctgcgag gttgccgaca ggtagccctg cgcgaacaac
      901 catcccgtga gcaccaacag cgccgccgaa atggcgtagg tgccggcgat catctgcttc
      961 cggccgatcg tgtcgaacag atggcctaac agcaccgggc ccagaaagtt tccgagcgcg
     1021 aacggcaaca ggtagatccc ggtgcgcgga gccggcacgt cgtagaatcg cgtgagaatg
     1081 agcacgtagg tgaaaaacac cgcgttgtac aagaacgcct gcgacgtgat cagcgccagg
     1141 cagagcaccg accgccgcca gtattgcttg aacatcacgc gcgccacgag gccgaacccg
     1201 aagctgcgcc gcggatggat gaccaacggc tcggtcgtgc acacctcgag cgtgcgaccc
     1261 agactccgct ccacccgcgc ttcgatcgcg tccatgatcc ggtgggattc gtgccccgat
     1321 ccgtgcgtca tgagccaccg cggactttcg gggatgctct tgcgcaggaa cagaatcagc
     1381 aggcctaacg cagcgccgat gccgaatccc acacgccagc ccagcgtcgg cggcaagatc
     1441 gccggatcca gaatgaccag cgtggaggcg gagccgatcg ccgcgccgat ccagtagctg
     1501 ccgttgacga tcaggtccgc ccggccgcga tacgtggccg ggatcaattc atcgatcgcc
     1561 gagttgatgg cggcgtattc cccgccgatg ccggcgccgg tcaagaatcg gaacgcgagg
     1621 aagctgctca ggttccacga gaacgccgtg agcaacgtgc ccaacagata gacggccagc
     1681 gtgacgaaga agagcttccg tcgcccgtaa cgatcggtca ggtgcccgaa cacgagcgaa
     1741 ccgatgaccg cgccgatcac gtacgtcgat ccgatttcgc cgatctgcgt cgcggtgaag
     1801 ttcatcaccg cc
//
LOCUS       DASUCJ010001125         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_440157, whole genome shotgun sequence.
ACCESSION   DASUCJ010001125 DASUCJ010000000
VERSION     DASUCJ010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440157"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFU90_13240"
     CDS             <1..835
                     /locus_tag="VFU90_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480802.1"
                     /translation="KTGRLLYASDFSNNVVLVYDYPAGTPAGTLTGFSNPQGLCADGG
                     DHNVFVANTGGENVLEYAWGATSPKATYGDSNEFPVACSVDPSTGRLAVADIFSPTTG
                     IGAVTICSQPSRCRVYVEPAGLESISAVAYLPNGDLYAAGTGSQGQLAGAYLAAGSRT
                     WTPVTFRASIGFLGAMQWDGKYLAIGDQQGPSGNTVIDRCSASGPNVSCNGGKVDLNS
                     SGDVVQFFIKYGDRGVVGADAASGGIGTWAYPQGGEPRKVIQGRGKYPLYVGMAVLRQ
                     P"
     gene            complement(870..1352)
                     /locus_tag="VFU90_13245"
     CDS             complement(870..1352)
                     /locus_tag="VFU90_13245"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HEU5480803.1"
                     /translation="MKYRAPSFRTLGTVATAALAVSALLVFARPVEMLVDGERIESDV
                     PPVTTASDKVFVPLRTIADALGAQTVVAGRDRIDVIRGTQSLRVHVGDSRATIDGVAV
                     TLKHAPFRVRGRVMVELHAVASAFNVRASYDPRGARVEVLTPGIGQAIAAPSTSRETQ
                     "
     gene            complement(1349..>2246)
                     /gene="ispG"
                     /locus_tag="VFU90_13250"
                     /gene_synonym="gcpE"
     CDS             complement(1349..>2246)
                     /gene="ispG"
                     /locus_tag="VFU90_13250"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5480804.1"
                     /translation="RSPVPIIADIHFQYQLALAALEAGVHGLRLNPGNIRDPKKVAEV
                     VTAAKAREIPIRVGVNMGSLAPDLEKTLGHTAEAMVESAMRHVEMLEEHGHDDIAISL
                     KAHDVPTTVAAYRLMDKRLRDRGTPYALHLGITEAGLLRDGTIKSSIGLGILLYEGIG
                     DTLRVSLADDPIEEVPVCWGILKSLGLREKGFEITACPSCGRAEISVLELGRAVEAIA
                     KSYSAPVKVAVMGCVVNGPGESKMADVGVAGRKGKGAIYRAGELVGTYPESELLGMLR
                     LEIEKVIAEKYPDFIHEVGARA"
BASE COUNT          394 a          758 c          704 g          390 t
ORIGIN      
        1 aaagaccgga cgactgctgt acgcatccga tttcagcaat aacgtcgtgc tggtatacga
       61 ctatccggcg ggaacgccgg ccggaacgct aaccggattc agcaatccgc agggcctgtg
      121 cgccgatggc ggcgatcata acgtgttcgt cgccaatacc ggcggcgaaa acgtcctcga
      181 atacgcttgg ggtgcgacga gcccgaaagc gacgtacggc gattcgaacg aatttccggt
      241 ggcgtgctcg gtcgatccga gcaccggaag actagccgtc gccgacatct tcagccccac
      301 gaccgggatc ggcgccgtaa cgatttgctc gcaaccgtcg cgctgcaggg tctacgtcga
      361 gcccgccgga ctcgagtcga tctcggccgt ggcctatctg ccgaacggcg acctctacgc
      421 cgccggaacc ggatcgcaag gccaactcgc cggtgcgtat ttggccgccg gcagccgaac
      481 gtggacgccc gtaacgttcc gcgcttcgat cggattcctc ggtgcgatgc agtgggacgg
      541 gaagtacctc gccatcggcg atcagcaggg cccatccgga aacacggtca tcgaccgctg
      601 cagcgcgtcc ggaccgaacg tgtcttgtaa cggcggcaag gtcgacttga actcttcggg
      661 cgacgtcgtt cagttcttca tcaagtacgg cgacaggggc gtcgtcggcg ccgatgccgc
      721 tagcgggggc atcggcacgt gggcgtatcc ccagggcggc gaaccgcgta aagtgattca
      781 gggccgcgga aagtatccac tgtacgtcgg catggccgtt ctgcgccagc cgtaacgccc
      841 gcgctttccg ggcctccagc ccgaagcgct tactgcgttt cgcggctggt cgaaggcgcc
      901 gcgatcgctt gaccgatgcc cggcgtgagg acttccacgc gggcaccgcg cggatcgtaa
      961 ctggcacgca cgttgaaggc actcgcgacg gcgtgcagct cgaccatcac ccggccgcgg
     1021 acgcggaacg gcgcgtgctt gagcgtcacc gcgacgccgt cgatcgtcgc gcgcgaatcg
     1081 ccgacgtgaa cgcgcagcga ctgcgtgccg cggatcacgt cgatgcggtc gcggccggcg
     1141 acgacggttt gcgcgccgag cgcgtcggcg atggtgcgca gcggaacgaa aactttatcc
     1201 gaggcggtcg tgaccggcgg gacgtcgctt tcgatccgct cgccgtcgac cagcatctcc
     1261 accggacgcg cgaagaccag caacgccgac accgccagcg ccgcggtggc caccgttccc
     1321 agcgtgcgaa agctcggcgc ccgatatttc acgcgcgcgc gccgacctcg tggatgaaat
     1381 ccggatactt ctcggcgata accttctcga tctcgagccg gagcattccg agcagctcgc
     1441 tctcgggata ggttcccacc agctcgccgg cgcgatagat cgcacccttg cctttgcggc
     1501 cggccacgcc gacgtcggcc atcttcgatt cgcccggccc gttgaccacg cagcccatca
     1561 ccgccacttt gacgggtgcg ctgtacgact ttgcaattgc ctcgacggcc cggcccagct
     1621 cgagtaccga aatctccgca cgcccgcacg acggacacgc cgtgatctcg aaaccctttt
     1681 cgcgaagccc gagcgacttc agaattcccc aacaaaccgg aacttcttcg atcgggtcgt
     1741 cggccaggga gacgcgcaac gtatcgccga tcccttcgta caacagtatt ccgagcccaa
     1801 tcgacgactt gatcgttccg tcgcgcagca gacccgcttc ggtaattccc agatgcagcg
     1861 cgtacggcgt tccgcgatcg cgcagccgtt tgtccatcag ccggtaagcc gccaccgtgg
     1921 tcggcacgtc gtgcgctttg agcgagatcg cgatatcgtc gtggccgtgc tcttcgagca
     1981 tttcgacgtg gcgcatcgcc gactcgacca tcgcctcggc ggtgtggccg agcgttttct
     2041 cgagatccgg cgcgagcgat cccatgttga cgcccacccg aatcggaatc tcgcgagctt
     2101 tggcggcggt gaccacttcg gcgacttttt tcggatcgcg gatgttgccg ggattcagcc
     2161 gcagcccatg tacacccgcc tcgagcgcgg caagcgcgag ctggtactgg aagtggatgt
     2221 cggcgatgat cggcaccggt gagcgc
//
LOCUS       DASUCJ010001126         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_929102, whole genome shotgun sequence.
ACCESSION   DASUCJ010001126 DASUCJ010000000
VERSION     DASUCJ010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929102"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFU90_13255"
     CDS             <1..1028
                     /locus_tag="VFU90_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU5480805.1"
                     /translation="PPSLLIPMIVLATAAAVIASQAVISGAFSVTREAIQLGYLPRME
                     VRHTSKETRGQIYLPWINRMLLVLTLAVVIGFRSSDNLGAAYGIAVVGTMTIDSILVM
                     IVFRRMWHWSKPHVAVVGGLFLAVDAAFLTANADKVEHGGWFPLVLGAVIFTMMATWR
                     RGRELVIREIRQSGLALEPFISSIAAHPPLRVPGTAVFLTANLDAVPHALLHNLKHNK
                     VLHERNVLLTVEVLETPTAEHDERVTVENLDDNFYKVTLRFGFAEDPDVPDTLESIQA
                     CGLPFELMDTTFFLSRESIVATDRPGMARWRDKLFVFMQRNATPATAFFQIPGNRLVE
                     LGTQVEI"
BASE COUNT          170 a          362 c          335 g          198 t
ORIGIN      
        1 tgccgccctc gctgctgatc ccgatgatcg tgctcgcgac cgcggccgcc gtgatcgcat
       61 cgcaggcggt catctccggc gcgttctcgg tgacgcgcga agcgatccag ctcggctatc
      121 tgccgcgcat ggaagtccgg cacacgtcga aagagacgcg cgggcagatt tatcttccgt
      181 ggatcaaccg catgctgctc gtgctgacgc tcgcggtcgt gatcggcttc cgctcgtcgg
      241 acaacctcgg cgccgcgtac ggcatcgcgg tggtcggcac gatgacgatc gactcgatcc
      301 tcgtcatgat cgtgttccgg cgcatgtggc actggtcgaa gccgcacgtc gccgtggtcg
      361 gcggcctgtt tctcgccgtc gatgccgcgt tcctgaccgc gaacgcggac aaggtcgagc
      421 acggcggctg gttcccgctc gtgctgggcg ccgtgatctt cacgatgatg gcgacgtggc
      481 ggcgcgggcg cgagctcgtg atccgcgaga tccgccagag cggccttgcg ctcgagccgt
      541 tcatcagctc gatcgcggca catccgccgc ttcgcgtacc cggtactgca gtatttctga
      601 cggcgaatct cgacgcagtc ccgcacgcgc tgctgcacaa cctcaagcac aacaaggtgc
      661 tgcacgagcg caacgtgctg ctgaccgtcg aagtgctcga aacgccgacc gccgagcacg
      721 acgagcgcgt gaccgtcgag aacctcgacg acaacttcta caaggtgacg ctccgcttcg
      781 gcttcgccga agatccggac gtacccgata cgttggagtc gatccaggcc tgcgggctcc
      841 cgttcgagct catggacacg acgttcttcc tcagccgcga aagcatcgtc gccaccgacc
      901 ggcccggcat ggcgcgctgg cgcgacaagc tcttcgtctt catgcagcgc aacgcgacgc
      961 cggcgacggc gttctttcag attccgggga atcggctcgt ggagcttggg actcaggtcg
     1021 aaatctagtt tcgtggtcct cgcccaagct tggacccggc gcctc
//
LOCUS       DASUCJ010001127         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_883429, whole genome shotgun sequence.
ACCESSION   DASUCJ010001127 DASUCJ010000000
VERSION     DASUCJ010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883429"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFU90_13260"
     CDS             complement(<1..567)
                     /locus_tag="VFU90_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480806.1"
                     /translation="MTFLLRMREASFGAVDAAVGPMTLDVRPRERAVRTCATPREAAV
                     TALLAAGIVKASRGTVLIDQYDPRVQPAHCKRIAAFVPHAALPFDGVAEFERYVVYRA
                     ALWDVDPMRAVAHAKLTLERLAGVHEAFAYPLAAALVGTPRLVVMDRPQSVYAEQILA
                     AVGDCAVFSTHLDASAARAFSGEARREIH"
     gene            588..>1533
                     /locus_tag="VFU90_13265"
     CDS             588..>1533
                     /locus_tag="VFU90_13265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5480807.1"
                     /translation="MAKATLTMLSNAEVAAKLLEIRTLMEMSGESFYKYMAYEKAAAS
                     VENAAPLADLVASGEHLKLPGIGKSIGAVVEQLVRTGTADNLDELHRRFPASILEVLG
                     VSGIGTKTAATLFADFGIASLADLETAIASGALAGIPRLGPKTIENWKRGILAYKGRQ
                     HRTPLAHALVIAHEAMSYLREGPPVDRLLPAGSLRRQEVTVGDIDLVCTSAEAAAVIA
                     HFTAWERAEAVLAEGPTKASIWLAGGLQIDLRVLPDHLYGNLLQHFTGSREHNIKLRE
                     HAVRKGLRVSENGILNLETGDAIVCSEEAGVYAALGMQF"
BASE COUNT          280 a          496 c          518 g          239 t
ORIGIN      
        1 gtgtatctcc cggcgagctt cgcccgaaaa cgcgcgtgcc gcggatgcgt cgagatgggt
       61 cgaaaacacc gcgcaatcgc cgacggcggc gaggatttgc tcggcgtaaa cggactgcgg
      121 ccggtccatc accacgagcc gcggcgtacc gacgagcgcg gcggccagcg gataggcgaa
      181 cgcctcgtga acgccggcga gccgttccag ggtgagcttt gcgtgggcca ccgcgcgcat
      241 cgggtcgacg tcccaaagcg cggcacgata gacgacgtag cgctcgaact ccgcgacccc
      301 gtcgaacggc agcgcggcgt gcggcacgaa cgcggcgatc cgtttgcagt gcgcgggctg
      361 cacccgcgga tcgtattgat cgatcagcac cgtgccgcgg ctcgccttca cgattcccgc
      421 cgccaacagg gcggtgactg cggcctcgcg cggcgtcgca caggttcgga ccgcgcgctc
      481 gcgggggcgc acgtcgagcg tcatcgggcc gacggcggcg tcgaccgcgc cgaacgatgc
      541 ttcgcgcatc cgtagcagga acgtcaaggc agtacccttc gacgcaattg gccaaggcta
      601 ccctcacgat gctttcgaac gccgaagtcg ccgccaagct gctggaaatt cgcacgctca
      661 tggaaatgtc gggcgaatcg ttttacaagt acatggctta cgagaaggcg gcggcgtcgg
      721 tcgaaaatgc cgcgccgctg gccgacttgg tcgcctcggg cgaacatctg aagcttcccg
      781 gcatcggcaa atcgatcggc gcggtggtag aacagctggt aagaaccggc accgccgata
      841 atctcgacga gctgcatcgg cgctttcccg catcgattct cgaagtgctc ggcgtgagcg
      901 gcatcggcac gaaaacggcg gcgacgttgt tcgccgactt cggcatcgct tcgctggcgg
      961 atttggaaac cgccatcgcg tcgggagcgc tggccggcat tccgcggctc gggcccaaga
     1021 cgatcgagaa ctggaagcgc ggcatcctag cgtacaaggg gcggcagcac cgcacgccgc
     1081 tcgcgcacgc gctggtgatc gcgcacgaag cgatgagcta cttgcgcgaa gggccgccgg
     1141 tagaccgcct gctgccggcc ggcagcttgc gacgtcagga agtgacggtc ggcgatatcg
     1201 acctcgtctg cacgtcggcg gaggccgccg ccgtgatcgc gcatttcacc gcgtgggagc
     1261 gggccgaggc ggtgctcgcc gagggcccta ccaaagccag catctggctc gccggcggac
     1321 tgcaaatcga cttgcgcgtg ctccccgatc atctgtacgg aaacttgctg cagcacttta
     1381 ccggatcgcg cgagcacaat atcaagctgc gcgaacacgc cgttcggaaa ggcttgcgcg
     1441 taagcgaaaa cggcattttg aatctcgaga ccggcgacgc gatcgtgtgc tccgaagagg
     1501 cgggagtcta tgccgcgctc ggcatgcagt tcg
//
LOCUS       DASUCJ010001128         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1143301, whole genome shotgun sequence.
ACCESSION   DASUCJ010001128 DASUCJ010000000
VERSION     DASUCJ010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFU90_13270"
     CDS             <1..415
                     /locus_tag="VFU90_13270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5480808.1"
                     /translation="TGGQASGGGSGTFSWPVTGTITSPFGWRSNPFGGSPEFHQGLDI
                     AAPTGTTVTAAAAGTVIMAQWYGGYGNYILIDHGGGYSTGYGHLSAIYVSNGQAVKRG
                     QAIGAVGSTGASTGPHLHFEVRIDGKPVDPAPRLH"
     gene            484..>1832
                     /locus_tag="VFU90_13275"
     CDS             484..>1832
                     /locus_tag="VFU90_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5480809.1"
                     /translation="MTLRVRALLGALIVVIIAAAGALALRYHAAATGAQPGIDVATTG
                     DVSGILDNADPNGPQMAALALRKLEDSYYKPVDPQILIAGETSALRGYLHAKKVPDPV
                     LPGEAASGDVNQDASRVADQLAYAQQHYSAKLGTNGRSELSEAALRGMMDSLKDPYTV
                     YLSPHEIQGLDESLSGGNFGGIGVYIYQLKDGRIVVQPIEQMPAARSGMKPGEVVDSV
                     GGAPVRGLSLDRVEQMIRGEPGTTVRITTHPYNAASHEHSYDIVREVIHVPSVRAKME
                     DGYEYIRLSDFGRTSGDEVRQALLDGQSHGAKGYILDLRDNGGGLLEAAVKISSLFVG
                     QGTIVSTIRRDGTRTTEEALGTAIGGMAPLVILVNKYTASASEITAGALQDYHLATLI
                     GTRTFGKGVVQSIYPMPDRGALKITTARYVTPLGRDIQHRGIEPDIVVDQSVDPSLI"
BASE COUNT          356 a          599 c          579 g          298 t
ORIGIN      
        1 caccggcggc caggcgagcg gcggcggatc ggggacgttc tcgtggccgg tgaccggcac
       61 gatcacctca cccttcggct ggcgctcgaa tccgttcggc ggctcgccgg agttccacca
      121 ggggctcgac atcgccgcgc cgaccggcac gaccgtaacg gcggccgccg ccggaaccgt
      181 tattatggcg caatggtacg ggggctacgg caactacatc ttgatcgatc acgggggcgg
      241 ctactcgacc gggtacggtc atttgagcgc gatttacgtc tccaacggcc aagcggtcaa
      301 gcgcggccaa gcgatcggcg cggtcggctc gaccggcgct tcgaccggtc ctcacctgca
      361 ctttgaggtg agaattgatg gaaaacccgt agaccctgcc ccccggcttc attaagacta
      421 atcgggtatc gttcgtggga aagaaccctt cggcggggta acccgggtag tgtaagagga
      481 gaaatgaccc tgagagtccg cgcgctgctc ggcgcgctta tcgtcgtcat catcgccgcg
      541 gccggtgcgc tcgcgctgcg ctaccacgcg gccgcaaccg gcgcgcaacc cgggatcgac
      601 gtcgctacca ccggcgacgt gagcggaatt ctcgacaacg ccgatccgaa cgggccgcag
      661 atggcggccc tcgccctgcg caaactcgaa gacagctatt acaagccggt cgatccccag
      721 atcttgatcg cgggcgagac ctcggctttg cgcggatacc tgcacgcgaa gaaggttccc
      781 gatccggtgc tgcccggcga agccgccagc ggcgacgtca atcaagacgc gagccgggtt
      841 gccgatcagc tggcttacgc ccagcagcat tattcggcca agctcggcac caacggccgc
      901 agcgagcttt ccgaagccgc cctgcgcggc atgatggatt cgttgaagga tccgtacacg
      961 gtctatcttt cgccgcacga aattcaaggg ctcgacgaat cgctgagcgg cggaaatttc
     1021 ggcggcatcg gcgtgtacat ctaccagctc aaagacggcc gcatcgtggt acaacccatc
     1081 gagcaaatgc cggcggcgcg gtcgggaatg aagccgggtg aagtcgtcga ttcggtcggc
     1141 ggcgcgccgg ttcgcggctt gtcgctcgac cgcgtcgaac agatgattcg cggcgagccc
     1201 ggcacgaccg tccggatcac gacgcacccc tacaacgccg cttcgcacga gcactcgtac
     1261 gacatcgtgc gcgaggtgat tcacgttccg tcggtacggg cgaagatgga agacggctac
     1321 gagtacatcc ggctctccga cttcggccga acctcgggcg acgaggtccg tcaagcgctg
     1381 ctcgacggcc agtcgcacgg cgctaagggc tacatcctcg acttgcgcga caacggcggc
     1441 ggcctgttgg aggcggccgt aaaaatctcg agcctcttcg tcgggcaagg gacgatcgtc
     1501 tcgacgatcc ggcgcgacgg aacccgcacc accgaagaag cgcttggcac ggcgatcggc
     1561 ggcatggctc cgctcgtcat cctggtgaac aaatacaccg cgagcgcctc ggaaatcacc
     1621 gccggcgcgc tgcaggacta ccatctcgca acgctgatcg gcacgcgaac gttcggaaaa
     1681 ggagtcgtgc agagcatcta cccgatgccc gatcgcggag ccttaaagat tacgaccgcg
     1741 cgctacgtga cgccgctcgg acgcgacatt cagcatcgtg gaatcgaacc cgacatcgtg
     1801 gtggaccaat ccgtcgatcc ttcgttaatc ga
//
LOCUS       DASUCJ010001129         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1326510, whole genome shotgun sequence.
ACCESSION   DASUCJ010001129 DASUCJ010000000
VERSION     DASUCJ010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326510"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="VFU90_13280"
     CDS             complement(<1..>1025)
                     /locus_tag="VFU90_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5480810.1"
                     /translation="VVHEPLAIDGCSDDPLPDAVSNPWKAMTSGGSTGRPKLIEANQP
                     GTIDPETRPLLMMQLNGTHLMPGPLYHNGPFVWTTTALLAGNHVVLGGKFDAETTLQL
                     IDRHHPDSMYVVPTMMARISKLPPEVRDRYDVSSLKVVWHLAAPCPPWLKEEWIDWLG
                     PTTIWELYAGTEAQAATIINGTEWLEHRGSVGRVNPEQFKIVRADGTDADADEVGEVF
                     MRPSADENVRTYRYVGADARRIDGGWESLGDMGRIDADGYLYLSDRQSDMILSGGANI
                     YPAEIEAALDEHPRVRSCAVIGLPDDDLGNRVHAIVHTDDGTPIAVDELRTHLAERLV
                     RYKIPRT"
BASE COUNT          172 a          344 c          319 g          190 t
ORIGIN      
        1 aatgtacgcg ggatcttgta gcgcacgagc cgttcggcga ggtgcgtgcg cagttcatca
       61 acggcgatcg gcgtgccgtc gtccgtgtgc acgatcgcgt gcacacggtt cccgagatcg
      121 tcgtcgggca ggccgatgac cgcgcacgac cgcactcgcg gatgctcgtc gagcgccgct
      181 tcgatctccg cgggatagat gttggccccg ccggacagaa tcatgtcgct ctgccggtcg
      241 ctcaaataga gatagccgtc cgcgtcgatg cgacccatgt cgccgagcga ttcccatccg
      301 ccgtcgatac gcctcgcgtc ggcaccgacg taacggtacg tccgcacgtt ctcgtcggcc
      361 gacggccgca tgaacacctc gccgacctcg tcggcatcgg catcggttcc gtcggcgcgc
      421 acgatcttga attgctccgg gttgacgcgc ccgaccgagc cgcgatgctc gagccattcg
      481 gtgccgttga tgatcgtcgc ggcctgcgct tccgttccgg cgtacagctc ccagatcgtc
      541 gtcggaccaa gccagtcgat ccactcttcc ttgagccacg gcggacacgg cgccgcgaga
      601 tgccacacga ccttcaacga cgacacgtcg tatcgatcgc gcacttcggg cggcagcttc
      661 gagatgcgcg ccatcatcgt cggcacgaca tacatcgagt cgggatgatg acgatcgatc
      721 agctgcaagg tcgtttcggc gtcgaacttg ccaccgagca cgacgtgatt gccggcgagc
      781 agcgcggtcg tcgtccacac gaacgggccg ttgtgataca gcggtcccgg catgaggtgc
      841 gtaccgttca actgcatcat gagcagcgga cgcgtctcgg gatcgatcgt tcccggctga
      901 ttcgcttcga tgagcttcgg ccgtccggtc gagccacccg acgtcatcgc cttccatgga
      961 ttcgacaccg cgtcgggaag cggatcgtcg gagcaaccgt cgatcgcgag cggctcgtgc
     1021 acgac
//
LOCUS       DASUCJ010001130         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_730434, whole genome shotgun sequence.
ACCESSION   DASUCJ010001130 DASUCJ010000000
VERSION     DASUCJ010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730434"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFU90_13285"
     CDS             complement(<1..1334)
                     /locus_tag="VFU90_13285"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480811.1"
                     /translation="MRIPNVLIILPILAVLLSACGGGGGSGSPAPNPIPSLPPAKDGK
                     YFKHIVILVQENRTFDNLFATFPGADGTTVGKTRNGGTLRLVKSPLVPYPDLSPSNAY
                     SPSWLADYNYGGMNGFDQVPVEGHPGSYVYRYIDPTQIVPYWTLAKQYVLFDHMFQTQ
                     GSGSFTAHQDLIAGGTAIDSSHQLIDFPSEHPWGCDAPGGTVTSLVTANDTFLHGQGP
                     FPCLSYRTLRDTLDAKGLTWRYYAPDVRGNDFGGALWVAFDAVKAVRHGPEWGKNVVW
                     PETSVFADIASDSLPAVSWVIPDFKNSDHPGGGSDTGPSWVAQVVNAIGKSPAWSSTA
                     IVVVWDDWGGWYDHVAPPKPITYGGLGFRVPAIVVSPYAKKGYVTHGVYELGSIVKLV
                     EDNWNLPRLGQTDERAASFLDDAFDFNQSPRPFVPIGAKYSRAYFEHQKPSN"
BASE COUNT          280 a          445 c          452 g          272 t
ORIGIN      
        1 tggttcgacg gcttctgatg ctcgaaatag gcgcgcgaat acttggcgcc gatcgggacg
       61 aacggccgcg gcgattggtt gaagtcgaag gcgtcgtcga gaaacgaggc ggcgcgttcg
      121 tcggtctgcc cgagccgcgg cagattccag ttgtcctcga cgagctttac gatgctgccc
      181 agctcgtaaa cgccgtgcgt cacgtaaccc ttcttcgcat acggcgaaac gacgatggcg
      241 gggacgcgaa atcccaaacc gccgtaggtg atgggtttcg gcggggcgac gtgatcgtac
      301 catccgcccc aatcgtccca cacgaccacg atcgccgtcg aactccacgc cggactcttc
      361 ccgatcgcgt taactacctg cgccacccac gacggcccgg tatcggagcc gccgccggga
      421 tgatcggagt ttttgaaatc gggaattacc caactcacgg cgggcagcga atcgctggcg
      481 atgtcggcga acaccgacgt ttcgggccac acgacgttct tgccccactc ggggccgtga
      541 cgtaccgctt tgacggcgtc gaacgctacc cagagcgctc ccccgaaatc gttgccccgg
      601 acgtcgggcg catagtagcg ccacgtcaac cctttcgcgt ccagcgtgtc gcgcagcgtg
      661 cgatagctca ggcacggaaa cggcccttga ccgtgcaaga acgtatcgtt cgcggtaacg
      721 agcgacgtca cggttccgcc gggggcgtcg catccccagg gatgctcgct cggaaaatcg
      781 atcaactgat gcgacgagtc gatcgccgtt ccgcccgcga tgagatcctg gtgggcggtg
      841 aaactcccgc tgccttgcgt ctggaacata tggtcgaaca gaacgtactg tttggccagc
      901 gtccagtacg ggacgatttg ggttgggtcg atgtagcggt agacgtacga gccggggtgt
      961 ccctcgaccg ggacttggtc gaagccgttc atgcctccat agttatagtc ggccagccac
     1021 gacggcgagt acgcgttgct cggcgataag tcgggatagg gaacgagcgg cgatttgacg
     1081 agccggagcg ttccgccgtt acgcgtcttt ccgacggtcg ttccatcggc ccccggaaag
     1141 gttgcaaaca gattgtcgaa cgtgcggttt tcctgcacga gaatgacgat gtgcttgaag
     1201 tactttccgt cttttgccgg cggcagcgac gggatgggat tgggcgccgg cgatccgctg
     1261 ccgccgcctc cgccgcaagc gctcagcaat accgcaagaa tcggaagaat gataagaacg
     1321 ttgggtatcc tcacagcccg acccttactc gaccgcagca aaagcccttc gaaatcgggc
     1381 gcccgtctac tcggtgttgg gcggaacgcg aaatcagaat agtcagcaac gctcgcgccg
     1441 cgtcgtcgt
//
LOCUS       DASUCJ010001131         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_409105, whole genome shotgun sequence.
ACCESSION   DASUCJ010001131 DASUCJ010000000
VERSION     DASUCJ010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409105"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFU90_13290"
     CDS             complement(<1..531)
                     /locus_tag="VFU90_13290"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU5480812.1"
                     /translation="MPIPLGARADRLTRVRALRTVKGRREHEQFAFEGPTLLAEAHRS
                     GTRIDELYVTADAYAATPLVRELEAGGTPTYEIAGASAAKLSDVDTPTGIVAVSAVRL
                     AAAGALPPDADGLVLVLGDLSDPANAGALLRSADAFGASAVLFGRLGVDPYHPKVVRG
                     AMGALFRLPLGLAEPAE"
     gene            complement(538..795)
                     /locus_tag="VFU90_13295"
     CDS             complement(538..795)
                     /locus_tag="VFU90_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480813.1"
                     /translation="MRVLADLSPRASYVVPVPQPRSSILTEIGWLAYFIAVFVAAVYL
                     LVGLLVPHHFNDKIAIALAALFAGLVMIGTRAWFTARSRSR"
     gene            797..>1008
                     /locus_tag="VFU90_13300"
     CDS             797..>1008
                     /locus_tag="VFU90_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480814.1"
                     /translation="MNDRPRLVGSERVFDGRVFDVRIDRLQYDDDPAVHRLDVVEHPA
                     SIGVVATTRSDDLVLVRQYRPAAGAF"
BASE COUNT          174 a          354 c          342 g          138 t
ORIGIN      
        1 ctccgcaggc tcggcgaggc cgaggggaag ccggaagagc gcccccatcg ccccgcggac
       61 gaccttcggg tggtatggat cgacgcccag ccgtccgaaa agcaccgccg acgctccgaa
      121 ggcgtcggcc gagcgcagca acgcgcccgc gttggcggga tcgctcagat cgccgagcac
      181 cagcaccagc ccgtcggcgt cgggcggcag cgcgccggcg gcggcaagcc gcacggctga
      241 aacggcgacg atgccggtgg gcgtgtcgac gtcggagagt ttcgcggcgc tcgcgccggc
      301 gatttcgtag gtcggagttc cgccggcttc cagctcgcga accagcggcg tggccgcgta
      361 cgcatcggcg gtgacgtaca gctcgtcgat gcgcgtgccg ctgcgatgcg cctccgccag
      421 cagcgtcggg ccttcgaacg caaactgctc gtgttcgcgc cggcccttca ccgtccgcag
      481 cgcgcgcacg cgggtcaatc ggtcggcgcg cgcgccgagc gggatcggca cgaaccccta
      541 acgcgaacgc gagcgcgcgg taaaccacgc acgcgtgccg atcatgacga gcccggcaaa
      601 aagcgcggcc agcgcgatcg cgattttgtc gttgaagtga tgcggcacca gcagcccgac
      661 cagcaggtac accgccgcca cgaacaccgc gatgaaatat gcaagccatc cgatttcggt
      721 gagaatcgac gaacgcggct gcggcaccgg aacaacatac gaggcgcgcg gcgacaagtc
      781 cgccaacact ctcacgatga acgatcgtcc ccgtctcgtc ggcagcgaac gcgtttttga
      841 cgggcgcgtg ttcgacgtgc gcatcgaccg cttgcaatac gacgacgatc cggccgtgca
      901 ccgtctggac gtcgtcgaac acccggcgtc gatcggcgtg gtggcgacca cgcgttcgga
      961 cgatctcgtc ctcgtgcggc agtaccgtcc ggccgccggg gccttctt
//
LOCUS       DASUCJ010001132         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_623252, whole genome shotgun sequence.
ACCESSION   DASUCJ010001132 DASUCJ010000000
VERSION     DASUCJ010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623252"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2589)
                     /locus_tag="VFU90_13305"
     CDS             complement(64..2589)
                     /locus_tag="VFU90_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480815.1"
                     /translation="MRFLIERARTLCAAFAASITFLVCTGAAPGPPGPVDVTGSVLAD
                     DGTPISGAAVTLSRQGQSVTAHTDAQGRFTVRVPSAGTYAVRAVAPGFAPLGERTASI
                     EGGAPTLTLNLARATTNSLTVIGRVRASAGDTVSTASAPSLTLSAQAAAASGATAVSS
                     MVWDQLSATPVIPLGGGSNATQAFALRGPDPTETLVDIDGHQVNNGNTGDFDLSLMDP
                     AALQDVQLVYGIAPSSLVGPNTIGGAINIQTLQPTLATHGLLRLFGGSYGSYGATAQA
                     SGSDGRFGYAMSFHGASSDGSVNQTVLAPPPPAAPPAPDETLQQVGSSSSGNSILTKL
                     RYQLGGDRGYGYVQLDMRDQTVVKDESALLTTYTPPGFAGGGGSADAVTGGLAPFAAP
                     PPGVFQSFAGTTLGAHQANYGLDAQLPLGGERLDGAPATMLQLSHLTTLDSQSVSGPG
                     EGAQPYLYNQRDLLGDDWLQIDHRFANGLLSFKYDLGTEALTSNYVQGQVTAELEHVA
                     VQPADAAGPPANVVNLDQTQRSAVLRYNGDPTSQIHFSLAGYYSNYSAFGSSFDPRGG
                     FVWTPTGNTAVRASVGTTFQTPQLSELVVFPAADRVPVGGIIYIGNPNLRPDHATEYD
                     VGAEQIFGKRGHQLHVSADLYQSNLRAPASQLVVDPIPHCQTKHNPVPCPLSYPVNAG
                     DGMYRGVDLRAEQQLGDGLRLRAGWSVDSSFLTAIPPEIQDGTLVIGEQSLGQPLHKA
                     YFGIDRDVPQGLVYGAQLNYEGAYNELDRTPYATLDAHVAYRRGGYEFGLYGTNLTNA
                     YAEPFTIVGGGILYGTVAGQPMTATNAYNLQGSKVVFVITRSI"
     gene            complement(2625..3275)
                     /locus_tag="VFU90_13310"
     CDS             complement(2625..3275)
                     /locus_tag="VFU90_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480816.1"
                     /translation="MLSAARVLRFCFVAVAGALAAAVAHVVIDVAGDYLLTRDAYDGL
                     AHNSRTVLLAAVGITVLMAAIRTIFDALDRRCGSRISLLALVRSELGRPWRFVGASVA
                     VATVLLMGMEAFDCSLVGHVDGLDDLFGGSLLLGLTTVAITGLLCGWGVHRIARRIAR
                     YEAPIAAFILAVLNLGSPAPAAGSTRQHMGEPRRVSRALLLSRRRHKRGPPLPIPG"
     gene            complement(3323..3874)
                     /locus_tag="VFU90_13315"
     CDS             complement(3323..3874)
                     /locus_tag="VFU90_13315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5480817.1"
                     /translation="MSRRELYRNRWLAVEQHAIVHPSGASGEHVLVVTPPTAAVVAVD
                     GEELVFARQPRFAARRPVVEIVKGGADDGESLADAARRELREELGASARRWSELGRLY
                     EIPSIVAVPVTLFLARDLDFGTPQPESVETIAPVRLSVGAAIEAASDGTIDDAVTVAA
                     IFRFALSGGYLTRSPNARLRREG"
     gene            3944..>4582
                     /locus_tag="VFU90_13320"
     CDS             3944..>4582
                     /locus_tag="VFU90_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5480818.1"
                     /translation="MRTSARMSFVTELFATTNVDKLRELGRRKILRRALTAKDLVAIG
                     LGTMIGGGIFTTIGTGVKGAGPAVIVSYLLAGLTSFFAALCYAELGAMVPVAGSAYTY
                     AYATMGKLFAWIIGFALIFEYGISAAPVAQQFSASIQDVFKSAGWALPAWAQQSNLVV
                     SGPWWNPGSWDLLHSQYDVVGALFVIGLSVLLSVGIRESATTNNIFVVLKISA"
BASE COUNT          752 a         1525 c         1539 g          766 t
ORIGIN      
        1 aaagtcgcac gcctcgctcg cgacaccctc gccgtcgctc aggggcgcga gcttcgggcg
       61 ggcctagatc gaacgcgtga tcacgaacac gaccttgctg ccttgcaggt tgtacgcgtt
      121 ggtggcggtc atcggctgcc ccgcaacggt accgtagagt attccgccgc ccacgatcgt
      181 aaacggctcc gcgtacgcgt tcgtgagatt cgtcccgtag agcccgaact cgtaaccgcc
      241 gcggcgatag gcgacgtgcg cgtccagcgt ggcgtagggt gtgcggtcga gctcgttgta
      301 cgcgccttcg tagttcagct gcgcgccgta gacgagccct tgcggtacgt cgcgatcgat
      361 cccgaagtac gctttgtgca gcggctggcc gagcgactgt tcgccgatca cgagcgtgcc
      421 gtcttgaatt tccggcggaa tcgcggtcaa aaacgagctg tccacgctcc acccggcgcg
      481 caggcgcagt ccgtcgccga gctgctgttc ggcgcgcaga tcgacgccgc gatacatgcc
      541 gtcgcccgcg ttgaccggat agctgagcgg gcacgggacc gggttgtgct tggtttgaca
      601 gtgcggaatc gggtcgacca ccagctgact ggcgggtgcg cgcaagttcg actgatagag
      661 gtccgccgac acgtgcagct gatgtccgcg cttgccgaag atttgttcgg cgccgacgtc
      721 gtactcggta gcgtggtcgg gccgcaggtt cgggttgccg atgtagatga ttccgcccac
      781 cgggacccga tccgccgccg gaaagaccac gagttccgat agctgcggcg tttgaaaggt
      841 cgttccgacc gaggcgcgca ccgcggtgtt gccggtcggc gtccacacga aaccgccgcg
      901 cggatcgaag ctgctgccga aggcgctgta attgctatag tatcccgcca gtgaaaagtg
      961 aatctggctc gtcgggtcgc cgttgtagcg caacaccgcc gaccgctgcg tctggtccag
     1021 attcaccacg tttgcgggcg gaccggccgc atcggcgggc tgcaccgcca cgtgctcgag
     1081 ctcggccgtt acctgcccct gtacgtagtt gctcgtcagc gcttcggtac ccaaatcgta
     1141 cttgaacgaa agcaatccgt tggcgaaacg gtgatcgatt tgcagccaat cgtcgccgag
     1201 cagatcgcgc tgattgtaga ggtacggctg ggcgccttcg cccgggcccg acaccgattg
     1261 cgaatcgagg gtcgtcaggt ggctcagctg cagcatcgtt gcgggcgcgc cgtcgagccg
     1321 ctcgccgccc agtggaagct gcgcgtcgag gccgtagttg gcctgatgcg cccccagcgt
     1381 cgtgccggca aagctctgaa agacccccgg cggcggcgcg gcgaacggcg ccaagcctcc
     1441 cgtcaccgcg tcggcgctgc cgccgccgcc cgcgaatccg ggcggcgtgt acgtcgtcaa
     1501 gagcgccgac tcgtctttaa cgacggtctg atcgcgcata tccagctgga cgtatccgta
     1561 gccgcggtcg ccgccgagct gatagcgcag tttcgtcaag atcgagttcc cggacgagct
     1621 gctgccgact tgctgcagcg tttcgtcggg tgccggcggc gccgccggcg gcggaggtgc
     1681 cagcaccgtt tggttgaccg aaccgtccga cgaagcgccg tgaaacgaca tcgcgtagcc
     1741 gaatcgtccg tcgctgccgg aggcctgcgc ggtcgcaccg tagcttccgt acgatccgcc
     1801 gaacagccgt aagagtccgt gcgtcgcgag cgtgggctgc agggtttgaa tgttgatcgc
     1861 tccgccgatc gtgttgggac ctaccagcga cgacggggcg atgccgtaca cgagctggac
     1921 gtcttgcaat gccgccgggt ccatcagcga taggtcgaaa tcgccggtgt tgccgttgtt
     1981 gacctgatgc ccgtcgatgt cgacgagggt ctcggtggga tcgggtcccc gcaacgcaaa
     2041 cgcttgcgtg gcgttgctgc cgccgcccaa cggaatcacc ggcgtcgccg aaagctggtc
     2101 ccaaaccatt gaggataccg cggtcgctcc ggaggccgcg gcggcttgtg cgctcagcgt
     2161 cagcgacggt gcggacgccg tcgataccgt atccccggcc gacgcgcgca cgcggccgat
     2221 gaccgtgagc gagttggtcg tcgcgcgggc caagttcaag gtgagcgtag gcgcgccgcc
     2281 ctcgatcgac gccgtgcgct cgcccagagg cgcaaatccg ggtgcgaccg cgcgcaccgc
     2341 gtacgttccc gccgacggaa cgcgcaccgt gaagcgtcct tgtgcgtcgg tatgcgccgt
     2401 caccgactga ccttgccgcg acagcgtgac cgcggcgccg gaaatcggcg tcccgtcgtc
     2461 ggcgaggacc gaaccggtaa cgtcgacggg ccccggcggt cccggcgcgg cgccggtaca
     2521 aaccaggaat gtgatggaag ccgcgaacgc cgcgcaaagc gtgcgcgcgc gctcgatcaa
     2581 gaaccgcatt gagatgctcc gatctcggct aaagaatgaa aaggctagcc ggggatcggg
     2641 agcggtggac cgcgtttgtg ccgccgtcgc gagagcagca gggcgcggct gacgcggcga
     2701 ggctcgccca tatgctgccg cgtggatccg gctgccggcg ccggcgaacc gagattgaga
     2761 accgcgagaa tgaatgcggc gatcggcgcc tcgtagcgcg cgatccgtcg ggcgattcga
     2821 tgaacgcccc agccgcatag caggccggtg atcgcgaccg tcgtgaggcc gaggagcagc
     2881 gagccgccga acaggtcgtc gagaccgtcg acgtgtccga ccaggctgca gtcgaacgcc
     2941 tccatcccca tcaggagcac cgtcgcgacg gcgaccgaag ctccgacgaa tcgccacgga
     3001 cgccccagct ccgagcgcac cagcgccagc agcgatatcc gggatccgca gcgccggtcg
     3061 agtgcgtcga agatggtgcg aatcgccgcc atcagaacgg tgatcccgac ggccgccagc
     3121 agcacggtgc gcgagttgtg cgcgaggccg tcgtaggcgt cgcgcgttag gaggtagtcg
     3181 cccgccacgt cgatgacgac gtgcgccacg gcggccgcga gcgcgccggc gacggccacg
     3241 aagcagaacc gcaaaacgcg cgcggccgaa agcatcgagc cgcgagtttc gtggtttctt
     3301 aacaactcct gcccggagaa ggtcatccct cccgacgcag cctggcgttc ggcgatctcg
     3361 taaggtaacc gccggaaagc gcaaaacgaa aaatggctgc gacggtcacc gcgtcgtcga
     3421 tcgtgccgtc ggatgccgcc tcgatcgccg cgccgacgct cagccgtacc ggcgcgatcg
     3481 tctcgacgct ctccggctgc ggcgttccga aatcgaggtc gcgcgccagg aagagcgtca
     3541 ccggaaccgc tacgatcgat ggaatctcgt acagccgtcc gagctccgac caccgccggg
     3601 cgctcgcacc cagctcttcg cgcagctccc gtcgcgctgc gtcggccagg ctctcgccgt
     3661 cgtcggcgcc gcctttgacg atttcgacga ccgggcggcg cgccgcgaaa cgcggctggc
     3721 gcgcgaacac gagctcttcc ccgtctaccg ctaccacggc ggccgtcggc ggcgtgacga
     3781 ccagcacgtg ctcgccggaa gcgccgctcg gatgcacgat cgcgtgctgc tccaccgcca
     3841 gccagcgatt ccggtacagt tcgcggcggc tcaagcgctc gtacggatcg tcgcgctcca
     3901 tcccacgcag cttggcgatt gccgggggag tggccccctt tgcgtgcgaa cctcggcccg
     3961 aatgtcgttc gtcaccgaac tatttgcgac cacgaacgtc gacaagctgc gcgagctggg
     4021 gcgccgcaag atcctacgcc gggcgcttac ggccaaggat ctcgtcgcca tcggtctcgg
     4081 aacgatgatc ggcggcggca tctttaccac catcggcacc ggggtcaagg gcgccggacc
     4141 ggcggtgatc gtctcgtacc tgttggccgg cctcacgtca ttcttcgccg cgctctgcta
     4201 cgccgagctc ggcgcgatgg ttccggtagc cggcagcgcc tacacctacg cgtacgccac
     4261 catgggcaag ctgtttgcat ggatcatcgg gttcgcgctg atcttcgagt acgggatcag
     4321 cgcggccccg gttgcgcagc agttctcggc gtcgatccag gacgtgttca agtcggccgg
     4381 ctgggcgctt ccggcgtggg cgcagcaatc caatctcgtg gtcagcgggc cgtggtggaa
     4441 ccccggcagc tgggatttgc ttcattcgca atacgacgtg gtcggcgcgc tgttcgtgat
     4501 cggactgagc gtcttgctct cggtgggaat tcgcgaatcg gctacgacca acaacatctt
     4561 cgtcgtcttg aagatctcgg cg
//
LOCUS       DASUCJ010001133         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_104103, whole genome shotgun sequence.
ACCESSION   DASUCJ010001133 DASUCJ010000000
VERSION     DASUCJ010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104103"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..981
                     /locus_tag="VFU90_13325"
     CDS             286..981
                     /locus_tag="VFU90_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480819.1"
                     /translation="MRSDGGSQSTTSLVPLAVAYILLIVISVGAIAILPPGTTSSGMA
                     ALDPFGPPELSRRFFASSADAVRVSAFFCFASAIPLVSYTAIIVTRLRLLGFRDAGQH
                     AAFAGGLAASGGLALAGLFLWAMSDAEISASLPITHTLHFLVFLSGGPAFAVGMGLLA
                     GGASMTLFDTGLVPNWVVRLGLALAVTGALSALGLLILPLTVAIPVTRVGGLVWLIAV
                     GALLPSRSSRSSG"
BASE COUNT          119 a          367 c          333 g          236 t
ORIGIN      
        1 aacacggcga acgccgtctg cccggtgtcg gtgtgtgttg ccgctgccgt cggttcgccc
       61 atgcccctca tccgtgtact cgagtcgacc gtgaatgata tcgcaccgcc gctgcgtccg
      121 gcatccgcga acgctggatt cgagcgtacc gcgggccgaa tcgacacttc gctcggcggc
      181 gaagtgttgc gtcggtcgtg aagatatctt gctgcgtgag catgcctcac gacacgggcg
      241 ccggctgtct gtagcttccc ctcagctcat tcgcgatggt gaccattgcg ttccgatggt
      301 ggcagccaat cgacgacgtc cctcgtcccg ttagccgttg cctacattct gctcattgtc
      361 atcagcgtgg gcgcgatcgc catcctgccg ccgggtacga cgtcgagcgg catggcggcc
      421 ctcgatccat ttggtcctcc cgagctgtcg cgtcggtttt tcgcatcgag tgcggacgcc
      481 gttcgcgtca gcgcgttctt ctgcttcgct tcggcgattc cgttagtcag ctacacggcc
      541 atcatcgtca cgcggctcag gcttctgggt ttccgcgacg ccggacaaca tgcggcgttc
      601 gccggcggcc tggcggcgtc cggcggtctc gccttggccg gcctcttcct ctgggcgatg
      661 tcggacgccg aaatcagcgc gtcgctgccc atcacgcaca ccctgcattt cctcgtcttt
      721 ctctcgggcg gacctgcgtt cgcggtgggc atgggactcc tcgcgggcgg cgcgtcgatg
      781 accctgttcg acacgggtct cgtgcccaac tgggtcgtcc gcctcggcct cgccctcgcc
      841 gtcacgggcg cgctgtccgc gttaggcctg ctgatcctgc ccctgacggt cgccattccg
      901 gtcacgcgcg tgggcggtct cgtgtggctc atcgcggtgg gggcgctctt gccgagccga
      961 tcgtcgcgct cgtccggctg agtccggcgg cttcggggcc gcccgttgtt gatcctgaca
     1021 ttccgcttgc ccgcgtgcgc acgatctacg aattg
//
LOCUS       DASUCJ010001134         1403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1219747, whole genome shotgun sequence.
ACCESSION   DASUCJ010001134 DASUCJ010000000
VERSION     DASUCJ010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219747"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VFU90_13330"
     CDS             <1..1388
                     /locus_tag="VFU90_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480820.1"
                     /translation="THTLVQDPTDKSVVYVYVSGLAQVRSNSEMAGCEAGDITDPNTA
                     LFRIEVIRVPLAHPEQAKIVSSPRIFNDLTHTATHGAAIADTANGADVVQARGFIDAI
                     GMPRTATAADSDKAAHLIADLLYETDTTRSRGLIDSLRTLGINFPSLSGGNRGPSQCH
                     DITVYPSSGFGAGACSGYGILLDIKDPMHPVRVDAQADSNFSFFHSATFNNDATKLLF
                     TDEWGGGVAPKCRATDPIDWGADAVFTLTGHTLAHAGYYKMPAAQTDVENCVAHNGNL
                     VPVPGRDIMVQGWYQGGVSVFDFTDPAHVKEIAYFDRGPIDPTKLVIGGYWGGYYYNG
                     YIYGSEIARGLDVFKLTPTPDLTQNEIDAAKLVHFDILNPQDQPKIVWPAAFPVARAY
                     VDQLKRDNGLSADRLTAIEGELTAAQRASGAKRTAALEKLATRLSRDAQGASDAKRVK
                     LLEGVVKGLEK"
BASE COUNT          265 a          466 c          454 g          218 t
ORIGIN      
        1 acacgcacac gctcgtgcag gacccgaccg acaagagcgt ggtctatgtg tacgtctcgg
       61 gtttggcgca ggtcaggtca aacagcgaga tggcggggtg cgaagcgggc gacatcaccg
      121 acccgaacac ggcgctcttc cgcatcgagg tgatccgcgt tccgttagcc caccccgagc
      181 aggcgaagat cgtgagctcg ccgcgcatct tcaacgacct cacgcatacc gcgacgcacg
      241 gcgccgcgat cgccgacacg gccaacggtg cggacgtcgt gcaggcgcgc ggattcatcg
      301 atgcgatcgg catgccgcgc acggcgacgg cggccgattc ggacaaggcg gcccacctca
      361 ttgccgacct gctgtacgag accgacacga cgcggtccag aggattgatc gactccctgc
      421 gcacgttagg catcaacttc cccagcctgt cgggcgggaa ccgcggcccg tcgcagtgtc
      481 acgatatcac cgtctatccg tcgtccggct tcggcgccgg cgcgtgctcg ggctacggca
      541 ttctgctcga catcaaggat cccatgcacc ccgtgcgcgt ggatgcccaa gccgattcca
      601 atttctcctt cttccactcg gccacgttca acaacgacgc gaccaagctg ctcttcacgg
      661 acgaatgggg cggcggcgtt gcgcccaagt gccgcgcgac cgatccgatc gattggggcg
      721 ccgacgccgt gttcaccctg accggccaca cgctcgcgca cgcgggctac tacaagatgc
      781 cggccgcgca gaccgatgta gagaactgcg tcgcgcacaa cggcaacctc gtgcccgtcc
      841 cggggcgcga catcatggtg cagggctggt atcagggcgg cgtgtcggtg ttcgacttca
      901 cggatccagc gcacgtgaaa gagatcgcct acttcgatcg cggcccgatc gacccgacga
      961 agctcgtgat cggcggctat tggggcggct actactacaa cggatatatc tacggctcgg
     1021 agatcgcgcg cggtctcgac gtcttcaagc tgacgccgac gccggatctg acgcagaacg
     1081 agatcgacgc ggcgaagctg gtgcacttcg atattctgaa tccgcaggac caaccgaaga
     1141 tcgtttggcc cgcggcgttc ccggtggcgc gcgcgtacgt cgatcaactc aagcgcgaca
     1201 acggtctctc cgccgatcgc ctaacggcaa tcgagggcga gctcacggcc gcccaacggg
     1261 cgagtggcgc caagcggacg gccgcgctcg agaagctggc gacccggttg tcgcgcgacg
     1321 cacagggcgc gagcgacgcg aagcgcgtga agctgctcga gggcgtggtg aaggggctgg
     1381 agaaatagcg ggaagcagag ctc
//
LOCUS       DASUCJ010001135         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1127780, whole genome shotgun sequence.
ACCESSION   DASUCJ010001135 DASUCJ010000000
VERSION     DASUCJ010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127780"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFU90_13335"
     CDS             <1..979
                     /locus_tag="VFU90_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5480821.1"
                     /translation="NYTAVLCALTHANERYASEGARSFSGQPVFYISRESHLAWVKIG
                     HQSGIGRSAVRQVPTDGTGRMDADALERMLDDDKARGCVPVLIVATAGTTNAAMIDPI
                     DRCAELARKHGLWLHVDAAWGGAVIASNRLRHVIAGIERADSVTIDAHKWFATTMGCG
                     MFITRHPLILSQTFQVSTGFMPSNTATVDPYVTTAQWSRRFLGVRLFLSLAAAGWDGY
                     AQHVERSVDLIDYLRGLLVERGWRIANDSPLGLLCIEPPPDSPDARTIGGRVLASGRA
                     WIAVAKYEGRDVIRICLTHGEATTEDIDVLVEALTDACRAAPTLTAVSA"
     gene            1030..1293
                     /locus_tag="VFU90_13340"
     CDS             1030..1293
                     /locus_tag="VFU90_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5480822.1"
                     /translation="MSASIRSTIVSQFEQVAAEQRHKLATLTDDRPLMESGLDSLAFA
                     IVVARLEDSLGIDPFTASEDAQFPVTFGDFVRMYETTYENAGA"
     gene            1286..>2076
                     /locus_tag="VFU90_13345"
     CDS             1286..>2076
                     /locus_tag="VFU90_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5480823.1"
                     /translation="MRNPLSLWERISEAGVDGDRSLWASDTSIALADLATASALSRPL
                     DELRGRSVLLSTSDQLAAALALIELDGIARRIVLCPPDLDPAHLPYVVKTAEVDAIAS
                     DRPADTFGSLGVECTVSIARSLHPAPVDRVARHDTEWILFTSGTTGVPKMVVHTLGTL
                     TGAIRRSGSLAGPLVWATFYDIRRYGGLQILFRALLGGGSLVLSAPGESAPAFLARTS
                     EHRVTHITGTPSHWRRALMSPAARTISPTYARLSGEIADQAIIDHL"
BASE COUNT          340 a          703 c          678 g          355 t
ORIGIN      
        1 gaactacacg gctgttctgt gcgcgttgac ccacgccaac gagcgctacg ccagcgaagg
       61 ggcgcgatcg ttcagcggcc aaccggtgtt ctacatctcg cgcgagagtc atctcgcctg
      121 ggtgaagatc ggtcaccagt cgggcatcgg ccgatcggcg gtgcgacagg taccgaccga
      181 cggcaccggc cgcatggatg ccgacgcgct cgagagaatg ctcgacgacg acaaagcgcg
      241 cggctgcgtt ccggtgctga tcgtcgcaac ggccggcacc accaacgccg ccatgatcga
      301 tcccatcgac cggtgtgccg agctcgcgcg aaagcacggg ctgtggctgc acgtggacgc
      361 cgcgtggggc ggcgcggtga tcgcgtcgaa tcggctgcgt cacgtgatcg ccggtatcga
      421 gcgtgcggac tcggtgacga tcgacgcgca caagtggttc gcgacgacga tgggttgcgg
      481 gatgttcatc acgcgccacc cgctgattct ctcgcagacc ttccaggtct cgaccgggtt
      541 catgccgtcg aacacggcca cggtggaccc gtatgtgacg accgcccagt ggtcgcgacg
      601 cttcctgggc gtgcgcctct tcctgtcgtt agccgcggcg gggtgggacg ggtacgctca
      661 acacgtcgag cgctccgtcg atctcatcga ctacctccgc gggctgctcg tggaacgagg
      721 gtggcgaatc gccaatgact cgccactcgg cctgctctgc atcgagccgc cgccggactc
      781 acccgacgct cgcaccatcg gtggacgcgt gctggcgtcg ggccgtgcct ggatcgccgt
      841 ggcgaagtac gagggacgcg acgtgatcag aatctgcttg acacatggcg aagcgacgac
      901 cgaggatatc gacgtgctcg tcgaggcgct caccgacgcg tgccgcgccg cgccgacact
      961 cactgccgta tctgcctgac gagcatcagc agcctacccc atcaacttcg acaacccgag
     1021 gaccattcga tgtccgcaag catccgatcg accatcgttt cgcagttcga gcaggttgcc
     1081 gccgagcagc gccacaagct ggcaacgctc accgacgatc gtcccctcat ggagtccggg
     1141 ctcgactcgc tcgcgttcgc gatcgtcgtc gcgcgcctcg aggactcgct tggcatcgat
     1201 ccgttcacgg cctccgagga cgcccagttt cccgtgacgt tcggcgactt cgtgagaatg
     1261 tacgaaacga cgtacgagaa cgcgggtgcg taacccgttg tcgttgtggg agcggatcag
     1321 cgaggcggga gtagacggcg accgctcgct ctgggccagc gacacgtcta tcgctctcgc
     1381 cgacctggca acggcgtcgg cgctgagccg tcccctcgac gagcttcgcg gccggtccgt
     1441 tctgctctcg acttcggacc agctcgcggc cgcgctcgcg ctcatcgagc tcgatgggat
     1501 cgcgcggcgg atcgtgctgt gcccaccaga tctggacccg gcgcacctgc cgtatgtggt
     1561 gaagacagcc gaggtcgacg ccatcgcgag cgaccggccg gcggacacgt tcggcagtct
     1621 gggcgtcgag tgcaccgtgt cgatcgcgcg aagccttcac ccggcgccgg tggaccgagt
     1681 ggcgcggcac gacactgagt ggattctctt cacgtccgga accaccggcg tgccgaagat
     1741 ggtggtgcac acactcggca cgctgacagg cgcgatcagg cgatccggaa gcctcgctgg
     1801 tccgctggtg tgggcgacat tctatgatat tcggcgatac ggcggactcc agatcctgtt
     1861 ccgcgcattg ctcggcggcg gctcgctcgt gctctcggct cctggagagt ctgcgccggc
     1921 gttcctcgcc cgcaccagcg agcaccgagt cactcacatc accggaacgc cgtcacattg
     1981 gcggcgcgcg ttgatgagcc ccgcggcgcg gacgatttcg ccaacgtacg cgcggctctc
     2041 cggcgagatc gccgatcagg ccatcatcga ccatct
//
LOCUS       DASUCJ010001136         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1173704, whole genome shotgun sequence.
ACCESSION   DASUCJ010001136 DASUCJ010000000
VERSION     DASUCJ010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173704"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..>1204
                     /locus_tag="VFU90_13350"
     CDS             235..>1204
                     /locus_tag="VFU90_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5480824.1"
                     /translation="MVAVGSRERLRFDTDWRFHLGHASDPARDFGYGAGEEFAKSGEL
                     FAPVSHADFDASAWRRLDLPHDWVVELPFVDDHGLTSYGFKPLGRAHPDTSIGWYRKT
                     FSIAAEDLGRRISLEFDGVFRDCVVALNGHLVAHNLSGYAPFAADIAPGLNYGGNNVL
                     VVRVDATEHEGWFYEGAGMYRHVWLVKTAPVHVARWGTFVHTDVDGARRAAIARVTTT
                     VDNDGDNPSRCSVDLQVIDGGGRVVQSARSAPVTIPAAGSVDVDQQLGIASPSLWSPD
                     APHLYTLRTRVMPNGGAECDRTETSFGIRTVQFDPDRGVLLNGEHVE"
BASE COUNT          188 a          407 c          405 g          204 t
ORIGIN      
        1 cggctgcttg tcagcgtcac gtatccggtg aaagttatgc gtcgcgtcca accactcgta
       61 cccatgtccg actccaagat atctcgccgc gatgccctgc tcacgggagc cgcatccgta
      121 ttaggcctcg ccaggatccc ctcgacgctc gacagcctgc gtgccgcgac cgggcgcgcg
      181 accggctccg cggccgagct accggctgac gcagagggcc ccagcggtga ggcgatggtg
      241 gctgtcggga gccgcgagcg cctgcgcttc gataccgact ggcgcttcca cctggggcac
      301 gcgagcgatc ccgcgcgcga cttcggctac ggcgccggcg aggagtttgc caagtccggc
      361 gagctcttcg cgccggtgtc gcacgccgac ttcgacgcgt cggcatggcg ccggctcgac
      421 ctgccccacg actgggtcgt ggagctgccg ttcgtcgacg accacggcct aacgagctac
      481 ggtttcaagc cgctcggccg cgcgcacccc gacacgagca tcgggtggta tcgcaagacg
      541 ttctccatcg ccgccgaaga cctcggacgc cgtatctcgc tcgaattcga tggcgtgttt
      601 cgcgattgcg tggtcgcgct caacggtcac ctggtggcgc acaatctgag cgggtacgca
      661 cccttcgccg cggacatcgc gccggggctg aactacggcg gcaacaacgt gctcgtcgtg
      721 cgcgtggatg cgacggagca cgagggatgg ttctatgaag gcgcggggat gtatcgccac
      781 gtgtggctcg tgaagaccgc gccggtgcac gtggcgcgtt ggggcacgtt cgttcatacg
      841 gacgtggatg gtgcgagacg tgccgccatc gctcgcgtca cgacaaccgt cgacaatgac
      901 ggcgacaacc catcgcggtg cagcgtcgac ttgcaagtga tcgatggcgg cggccgggtc
      961 gtgcagtctg cgcgatcggc gcccgtcacc attccggcag cgggctcggt cgacgtcgat
     1021 caacagcttg ggatcgcgtc gccatcgttg tggtcgcccg acgcgccaca cctctacacg
     1081 ctgcgcacgc gtgtcatgcc taacggaggc gccgagtgcg atcgcacgga gacgtcgttc
     1141 ggcatccgca ccgtgcagtt cgaccccgat cgcggcgtgc tcctgaacgg cgagcacgtg
     1201 gaga
//
LOCUS       DASUCJ010001137         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_211160, whole genome shotgun sequence.
ACCESSION   DASUCJ010001137 DASUCJ010000000
VERSION     DASUCJ010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211160"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU90_13355"
     CDS             <1..197
                     /locus_tag="VFU90_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480825.1"
                     /translation="TGYGIADIALFGYTHCAEDGGFDLSRYPNIVAWLGRVRAQPGFV
                     PMPAYAPENVALIEQSREPA"
     gene            237..>1023
                     /locus_tag="VFU90_13360"
     CDS             237..>1023
                     /locus_tag="VFU90_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5480826.1"
                     /translation="MAAIPNPIPARHKNVNRAEVCDAKFVEFVQGWHGEKHRAPRADE
                     AVLPGSLCTAKDFTDLFESQLISRHLDLMARVLRVKNKVFYTIGSSGHEGNAMIARLT
                     RHTDPAFLHYRSGAFMAERFRKLPNMDPIMDSALSFAASADDPASGGRHKVWGSKPLW
                     VLPQTSTIASHLPKAFGTAVAIEQSRRIAHALPIPDDSIVICSFGDASSNHATAQTAF
                     NTAQWTAYQKLPAPILFVCEDNGIGISVKTPKDWIGASFSGRRD"
BASE COUNT          175 a          344 c          325 g          179 t
ORIGIN      
        1 cgacgggtta cggcatcgcg gacatcgcgc tgttcgggta cacgcactgc gccgaagatg
       61 gcggctttga tctctcgcgc tatccgaaca tcgtcgcgtg gctcgggcgc gtgcgcgcgc
      121 agccggggtt tgtgccgatg ccggcttatg cgccggagaa tgtcgcgctg atcgagcaat
      181 cgcgcgaacc tgcctgaacc gtcggaatga cgaacacttt tcgttgatcg tagaacatgg
      241 ccgccatccc taaccccatc cccgcccgcc acaagaacgt caaccgcgcc gaagtctgcg
      301 acgcgaagtt cgtcgagttc gtgcagggct ggcatggcga gaagcatcgc gcgccgcgcg
      361 ccgacgaggc cgtgttgccg ggcagcctct gcaccgcgaa ggatttcacc gatctcttcg
      421 aatcgcagct gatttcgcgc catctcgatc tcatggcgcg cgtgctgcgc gtcaagaaca
      481 aggtgttcta cacgattgga tcgagcggcc acgaaggcaa cgcgatgatc gcgcgcctga
      541 cgcggcacac cgatcctgcg ttcctgcact accgctcggg tgcgttcatg gccgagcgct
      601 tccgcaagct gccgaacatg gatccgatca tggattcggc gctttcgttt gcggcgagcg
      661 ccgacgatcc cgcgtccggc ggccggcaca aggtctgggg ctcgaagccg ctctgggtgc
      721 tgccgcagac ctcgacgatc gcctcgcact tgccgaaggc gttcggcacg gccgtcgcga
      781 tcgagcagtc gcggcgcatc gcgcacgcgc tgccgatccc ggacgattcg atcgtgatct
      841 gctcgttcgg cgacgcgtcg tcgaaccacg cgaccgcgca gaccgcgttc aacacggcgc
      901 agtggaccgc ttaccagaaa ctcccggcgc cgatcctgtt cgtctgcgag gacaacggca
      961 tcggcatctc ggtcaagacg ccgaaggact ggatcggtgc gagcttcagc ggccgccgcg
     1021 acc
//
LOCUS       DASUCJ010001138         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_455483, whole genome shotgun sequence.
ACCESSION   DASUCJ010001138 DASUCJ010000000
VERSION     DASUCJ010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455483"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFU90_13365"
     CDS             complement(<1..658)
                     /locus_tag="VFU90_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480827.1"
                     /translation="MFLGRFWALVLKEFRQARRDRRIAFVAVGAPTIQLLVFGFVLSA
                     TVTNLPLGVVNENATPESRDLVAALTESKSFRLGGYYASVDDLSAAISRGELDAGVVI
                     PYDYARNLQRGLPVTVQFFMNATNANTAAIGQGYVAGVLGSYVNQLAISGIHVRRPPP
                     GVRPVASRGVVVLKTDYLYNPGLVGSWFMVTGILGMLLILNGSLLSSTMMVKEKSAGT
                     I"
     gene            complement(661..1767)
                     /locus_tag="VFU90_13370"
     CDS             complement(661..1767)
                     /locus_tag="VFU90_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480828.1"
                     /translation="MRRILAQTHKELIQIVRDRMALILALVLPAFLLLLMSTAISLTV
                     DEMPMIVQNFDGSSASVRLLDAFRASLTFKIVPWRVDENPERALSSGVARAALIVPEH
                     FGRDIARGRATPVQLLVDASDANTAQQVSGYAQQVVNSYNIGLYATGRSAPVVAAIRL
                     WFNPGRDSKKFYGPGIFVLALTLFPTLLAALSVSKEGEEKTILQVYVSNISAHEYLLG
                     KIAAIMVVFACEAIILMALLLTYFGLHLAGDPTPFLTASILYAFCVASFGVMVGASIP
                     NQLGAVAVVALGGFLLVFILSGLLFPIENIPPALRWMSNFVWGRYYIEVVRDALLQGG
                     GWPAMWYKVGIIGLIGSAFYSVAWWNMRAMQVRD"
     gene            complement(1767..>3345)
                     /locus_tag="VFU90_13375"
     CDS             complement(1767..>3345)
                     /locus_tag="VFU90_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480829.1"
                     /translation="YLELFAMRPFVDRLAAQLSGGMKQKLALACALVPEPKVLLLDEP
                     TTGVDPVSRREFWDALTRLAIEGLTILIATPYLDEAERCNRVALMHLGEIKELQTPDG
                     LRQGLHAKRLEVRPSDLRAAQSALSGVEKDGVVDVQRFGDHFDVLARDDRVDEVKREV
                     EAGLLAAGVTVEEVRVDQPTLENVFVASLRASGQDTMESPFPARRDHHVLRGKVAIGA
                     TDLDKQFGSFVAVDGVNLDVRYGEIYGLLGANGAGKTTTIKMLCGLLDPSDGSVQLAG
                     ATGGRRRDVAVRQQVGYMSQKFSLYDDLSIDENLDFFAGVYGVPAAERAEKIAWVLEF
                     SGLQGKEKEVTGRLPGGWKQRVAFGSSIMHEPSVLFLDEPTSGVDPIARRAFWRVIVG
                     LADAGAAVLVTTHYLEEAEQCNRLGFMAGGKLVLEGSPGEIKAREPGHLLEFRTDKPQ
                     RAADLLQAGGQRWRVSVFGDRVHLVVDDVAAGLESTTQRLLANGVAVHDAREIPFSLE
                     DVFIQAVERVRAPQEVA"
BASE COUNT          624 a         1103 c         1087 g          531 t
ORIGIN      
        1 cgatcgtgcc ggccgacttt tctttgacca tcatcgtcga cgacagcagc gagccgttga
       61 ggatcagcag cattcccaag atgcccgtga ccatgaacca cgatccgacc aagcccggat
      121 tgtagagata atccgtcttc aaaacgacca cgccgcgcga cgcaaccggt cggactcccg
      181 gcggcggccg ccggacgtga atgccgctga tcgccagctg gttgacgtac gaaccgagca
      241 cgccggcaac atagccttgc ccgatcgcag ccgtgttcgc gttggtggcg ttcatgaaga
      301 actgcacggt caccggaagg ccgcgctgca agttacgcgc gtagtcgtag ggaattacga
      361 cgccggcgtc cagctcgccg cggctgatgg cggcgctcaa gtcgtcgacc gaagcgtagt
      421 acccgcccaa gcggaagctc ttgctctcgg tgagcgccgc gaccaagtcg cggctctccg
      481 gcgtcgcgtt ctcgttcacc acgcccagcg gaaggttcgt taccgtcgcg ctgagcacga
      541 agccgaaaac gagcagctgg atcgtcggcg cgccgaccgc cacgaacgcg atccgtcgat
      601 cgcgacgcgc ctgccggaac tccttgagga cgagcgccca aaagcggccc aagaacatcg
      661 ctagtcccgt acctgcatgg cgcgcatgtt ccaccaggcg accgagtaga acgccgagcc
      721 gatcaggccg atgatcccga ccttgtacca catcgcgggc cagcccccgc cttgcagcag
      781 cgcgtcgcgc acgacctcga tgtagtagcg cccccagacg aagttcgaca tccagcgcag
      841 cgcgggaggg atgttctcga tcgggaagag cagccccgaa agaatgaaga cgagcaagaa
      901 tccgcctagc gcgacgaccg cgaccgcgcc aagctggttg ggaatcgagg cgccgaccat
      961 cacgccgaac gacgccacgc agaaggcgta gaggatcgac gcggtcagaa acggcgtggg
     1021 atccccggcg aggtgcagcc cgaagtaggt aaggagcaac gccatcaaaa tgatcgcttc
     1081 gcacgcgaag acgaccatga tcgccgcgat cttcccgagc agatactcgt gcgccgagat
     1141 attcgagacg tacacctgca agatcgtttt ctcttcgccc tccttcgaga ccgagagggc
     1201 ggcgagcagg gtggggaaga gcgtgagcgc cagcacgaag atgcccggtc cgtagaactt
     1261 cttggaatcg cgtccggggt tgaaccacag ccgaatcgcc gcgaccaccg gcgccgaacg
     1321 tcccgtcgcg tagagcccga tgttatacga attgacgact tgctgcgcgt agccggagac
     1381 ctgctgtgcg gtgttggcgt cggacgcgtc gacgagcagc tgcaccgggg tggcgcgtcc
     1441 gcgggcgatg tcgcggccga aatgctcggg aacgatcagc gccgcccgcg cgacgcccga
     1501 cgagagcgcg cgctcggggt tctcgtcgac ccgccacggc acgatcttga acgtgagcga
     1561 agcccggaac gcgtcgagca ggcgtaccga cgccgaggaa ccgtcgaagt tctgcacgat
     1621 catcggcatt tcgtcgacgg tcagcgaaat cgcggtgctc atcaagagca gcaagaaggc
     1681 gggaaggacg agcgcgagga tcagcgccat gcggtcccgc acgatttgaa tgagctcttt
     1741 atgcgtctga gcgaggatgc ggcgcatcag gcgacttcct gcggcgcgcg tacgcgctcg
     1801 accgcttgga tgaaaacgtc ttccagcgag aacggaatct cgcgggcgtc gtgcacggcg
     1861 acgccgttcg cgagcagacg ctgcgtggtc gactccaaac cggccgcaac gtcgtcgacg
     1921 acgagatgga cgcggtcgcc gaataccgag acgcgccagc gctggccccc ggcctgcagc
     1981 aaatcggcgg cgcgctgcgg tttatcggtg cgaaactcga ggaggtgtcc gggctcgcgg
     2041 gctttgattt cgccgggcga tccttcgagc acgagctttc ccccggccat gaaaccgaga
     2101 cgattgcatt gctcggcctc ttcgagatag tgcgtggtga cgagcacggc cgcgcccgcg
     2161 tccgccaatc cgacgatgac gcgccaaaac gcgcgccgcg cgatcggatc gacgcccgag
     2221 gtgggctcgt cgaggaacag tacgctcggc tcgtgcatga tcgacgagcc gaacgcgacg
     2281 cgctgcttcc agccgcccgg caaacgcccc gtgacctctt tctccttccc ctgtaggccg
     2341 gagaactcga gtacccaggc gatcttttcg gcgcgttcgg ctgccggcac gccgtagacg
     2401 ccggcgaaga aatccagatt ttcgtcgatc gataagtcgt cgtagagcga aaatttttgc
     2461 gacatatacc cgacttgctg gcggacggcg acgtcgcggc ggcggccgcc cgtcgcgccc
     2521 gcgagctgca cgctaccgtc gctgggatcg agcaggccgc agagcatttt aatcgtggtc
     2581 gtcttgcccg cgccgttggc gccgagcagc ccgtagattt cgccgtagcg tacgtccagg
     2641 ttgacgccgt cgacggcgac gaagctgccg aactgcttgt cgagatcggt cgcgccgatc
     2701 gcgactttgc cgcgcaggac gtggtggtcg cgacgcgccg gaaacggcga ctccatcgtg
     2761 tcttgccccg acgcgcgcag cgaagcgacg aagacgttct cgagggtcgg ctggtcgacg
     2821 cgtacctcct cgaccgtcac gccggcggcc agcaggccgg cctcgacctc gcgtttgacc
     2881 tcgtcgaccc ggtcgtcccg cgccagcacg tcgaagtggt cgccgaagcg ctgcacgtcg
     2941 accacgccgt ctttctcgac gccgcttagc gcgctctgcg cggcgcgcag atcgctgggc
     3001 cgcacttcca ggcgcttggc gtggaggccc tggcgcagac cgtcgggcgt ctgcagctct
     3061 ttgatctccc cgagatgcat gagcgcgacc cggttgcaac gctccgcttc gtcgagatag
     3121 ggcgtcgcga tgaggatcgt cagcccttcg atcgccagtc gcgtgagcgc gtcccagaac
     3181 tcgcggcgcg acaccggatc gacgccggtg gtcggttcgt cgagcagcag cactttcggt
     3241 tcgggtacca gcgcgcaagc cagcgcgagc ttttgcttca tgccgccgct gagctgcgcg
     3301 gccagccggt cgacgaacgg gcgcatcgcg aacaactcga gatag
//
LOCUS       DASUCJ010001139         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_211173, whole genome shotgun sequence.
ACCESSION   DASUCJ010001139 DASUCJ010000000
VERSION     DASUCJ010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211173"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1040)
                     /gene="ligD"
                     /locus_tag="VFU90_13380"
     CDS             complement(48..1040)
                     /gene="ligD"
                     /locus_tag="VFU90_13380"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5480830.1"
                     /translation="MSAPERVPVTVGARRLSLSNLDKVLWPRDGYTKGDLIDYYRAVA
                     GVILPFLKDRPLTLQRFPDGIDGPSFFEKHLPKGVPDWVDRVTTSSAEGGKRVTYVVC
                     NEEATLVYAANLASIPLHAWTSRVETLDEPDFVFFDVDPGERCTLKTLASVTLAIRDA
                     LAEIGLQALVKTSGGMGLHVFVPLSAGYSYESAKLFAELLARHVAAGRDETVTLQRAI
                     GKRRPEAVYLDFVQVGRGKTYVVPYCVRARDGAPVSTPLEWGEVEAFARKRGSIDPSE
                     EFGKFTMRTTPRRLKQLGDVWGGKAWKKQRLEPAIARAQKRWGFGGRDTAPENG"
     gene            complement(1037..>1782)
                     /gene="ligD"
                     /locus_tag="VFU90_13385"
     CDS             complement(1037..>1782)
                     /gene="ligD"
                     /locus_tag="VFU90_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5480831.1"
                     /translation="FKSVPIVIDGEIVSLDKQGRSEFQRLQEAQKKTTVLSFAAFDLI
                     YADGRDLRKLPLEERKALLERTLRDNGGPVLYSKHIVGKGKALFAVAQREKLEGIVGK
                     KRTSTYQERRSRDWVKVKAQLRQEFVVGGWTEPRGSREGFGALLLGAYADGAFRYVGH
                     VGTGFSQKVLLELHGRLRGLARKTSPFDTPVEANVRAHWVKPQLVVEVRFTEWTRDLL
                     LRHPAYLGLREDKAAAGVGLELPSNRRPS"
BASE COUNT          307 a          622 c          538 g          315 t
ORIGIN      
        1 aagttgatgg acccagacca aattgcgtgc gccatagtct gcccatttta cccgttttcg
       61 ggcgccgtgt ctcggccccc gaagccccag cgcttctggg cgcgggcgat cgcaggctcg
      121 aggcgctgct tcttccaagc cttgccgccc cacacgtccc ccaactgctt caggcgccgg
      181 ggcgtcgtcc gcatcgtgaa tttgccgaac tcctccgacg ggtcgatcga gccgcgtttg
      241 cgcgcgaacg cctccacttc gccccattcc agcggcgtcg acaccggagc gccgtcgcgc
      301 gcgcgcacgc aatacggcac gacgtaggtt ttcccgcgac cgacctggac gaaatcgagg
      361 tacaccgcct ccggccggcg tttcccgatc gcgcgctgca gcgttacggt ttcatcccgg
      421 ccggccgcga cgtgccgcgc gagcagttcg gcaaacagct ttgcgctttc gtacgaatat
      481 ccggccgaca gcggaacgaa cacgtgcaat cccatcccgc ccgacgtctt caccaacgcc
      541 tgcagaccga tctcggcgag cgcgtcgcgg attgctaggg tcaccgatgc gagcgtcttg
      601 agcgtgcatc gctcgcccgg atcgacgtcg aagaacacga aatccggctc gtcgagcgtc
      661 tccacgcgcg acgtccacgc gtgtaacgga atcgacgcca agttggcggc gtacacgagc
      721 gtggcttcct cgttgcagac gacgtacgtc acgcgtttcc cgccctcggc cgacgacgtc
      781 gtcacgcgat cgacccagtc gggaacgcct ttgggcaggt gtttctcgaa gaacgacggc
      841 ccgtcgatgc cgtcgggaaa gcgctgcagc gtcagcggcc ggtccttcaa gaatggcaag
      901 atgacgccgg ctacggcgcg atagtagtcg atcaaatcgc ctttggtgta accgtcgcgc
      961 ggccagagca ccttgtcgag attcgagagc gacaggcggc gcgcgccgac cgttacggga
     1021 acgcgctcgg gtgcgctcac gacggtctcc ggttcgacgg aagctcgagg ccgacgccgg
     1081 ccgcagcctt gtcttcgcgc aatcccaaat aagcggggtg ccggagcagc agatcccgcg
     1141 tccattccgt gaaacgaacc tcgacgacca gctgcggctt tacccagtgc gcccgcacgt
     1201 tcgcctcgac gggcgtgtcg aacggtgacg ttttgcgcgc cagcccgcgc aggcgcccgt
     1261 gcagctcgag caggactttc tgcgaaaatc cggttcccac gtgcccgacg taccggaagg
     1321 caccgtcggc atacgcgccc agcagcaaag ccccgaaacc ttcgcggctg ccgcgcggct
     1381 cggtccagcc gccgaccacg aactcctgac gcaactgcgc cttcaccttg acccagtcgc
     1441 gcgaacgccg ctcctgatac gtcgaggtgc gtttctttcc gacgatcccc tccagcttct
     1501 cgcgttgcgc gaccgcgaag agcgccttgc ccttgccgac gatgtgtttg gagtacagaa
     1561 cgggtccgcc gttgtcgcgc agcgttcgct cgagcaaggc tttgcgctcc tccagcggca
     1621 gcttgcgcaa atcccggccg tcggcgtaga tcagatcgaa cgcggcaaac gaaagcaccg
     1681 tcgttttctt ctgcgcttcc tggaggcgct gaaactcgga gcgaccctgc ttgtcgaggc
     1741 tcacgatttc gccgtcgatg acgatcggaa cgctcttgaa gg
//
LOCUS       DASUCJ010001140         1494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_302536, whole genome shotgun sequence.
ACCESSION   DASUCJ010001140 DASUCJ010000000
VERSION     DASUCJ010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302536"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>1494
                     /locus_tag="VFU90_13390"
     CDS             136..>1494
                     /locus_tag="VFU90_13390"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEU5480832.1"
                     /translation="MNAFRIRTAGAAVALALFAALPLTAPAAADPVTRLLARSRAALG
                     GSALDRVKTLELHGKLVAGGISGTGIAWEEIGGTRFAESYDLAPLAGGDGYDGSEVWN
                     RDGSGLVWVDGGQSGRAQEITQAFVSDYALWSPGRGGATVTDAGAKSAAGRTYDVLRV
                     TAPHSAAPFDLWLDRTTHLPARVSQTIGPNSSVTTYSQYRPVDGLMIPYVSHTESNDG
                     NSSDSSVTQVTANPAGAAANLAKPASDVHDFSIAGGATQTSVPFDLAENHVYLNVMLN
                     GKGPYRFIFDTGGANIVDPAVAKEIGATGHGSLQGGGVGSTTESVSFAKVDTLQVGDA
                     IVKDQLFGVAPTRMGFGMSAGRPVDGLIGFEVLSRFVTTFDYGNNRVVLAMPGTAAPA
                     NGAHVVPFVLDGRQPQFGCDIDGIAAQCTVDTGARDSITLMGPFMAAYPQVVPKTLTA
                     AGV"
BASE COUNT          253 a          498 c          509 g          234 t
ORIGIN      
        1 gggcgagctg ggcggctacc gctggggact cgcgcgcaag cgcgccctca tcgaccgcga
       61 acgcgcgcac cggaccgcgg ccgccggggc ggcgcgcgag gaaacaattg cgccgcttgc
      121 tgcgtaccgg agggcatgaa cgcctttcgc attcgcacgg ccggggcggc cgtagcgctc
      181 gcgctgtttg cagcgctccc cctgaccgcg ccggccgctg ccgatccggt aacccggctg
      241 ctcgcacgat cgcgcgccgc tctcggaggc tccgccctcg atcgcgtcaa gacgctcgag
      301 ctgcacggaa aactcgtcgc cggcggaatt tccggcacgg gaatcgcgtg ggaagaaatc
      361 ggcggaaccc gtttcgccga gtcgtacgac ctggcgccgc tggcaggcgg cgacggatac
      421 gacggcagcg aggtgtggaa tcgcgacggt tccgggctgg tctgggtcga cggcgggcag
      481 tccggccgcg cccaggagat tacccaggcg ttcgtgtcgg actacgccct ctggagtccc
      541 ggccgcggcg gcgcgacggt aacggacgcg ggcgcgaaga gcgcggcggg aaggacgtac
      601 gacgttttac gcgtgaccgc gccgcattcg gccgcgccgt tcgatctctg gctcgatcgc
      661 acgacgcatt tgccggcgcg cgtctcgcag acgatcggtc cgaactcgag cgtcacgacg
      721 tattcgcagt atcgtccggt cgacggcctg atgattccct acgtgtcgca taccgaatcg
      781 aacgacggaa actcgagcga ctcgagcgtt acgcaggtga cggccaatcc ggccggcgcc
      841 gccgcgaact tagcgaagcc ggcgtctgac gttcacgatt tttcgatcgc cggcggcgca
      901 acccagacga gcgtcccgtt cgatctcgcc gaaaaccacg tgtacctcaa cgtaatgctc
      961 aacggcaagg ggccgtaccg tttcatcttc gatacgggcg gtgcgaacat cgtcgatccc
     1021 gcggtcgcca aagaaatcgg cgcgaccggg cacggatcgc tgcaaggcgg cggcgtcgga
     1081 tcgaccaccg aatcggtcag cttcgcgaag gtcgacacgc tgcaagtggg cgacgcgatc
     1141 gtcaaggacc agctgttcgg cgtagcgccg acgcgaatgg gcttcgggat gtcggccgga
     1201 cggccggtcg acggtttgat cggtttcgaa gtgctgtcgc gcttcgtcac gacgttcgac
     1261 tacggcaata accgcgtcgt actcgcaatg cccggaacgg ccgcgcccgc aaacggcgcg
     1321 cacgtggtac cgttcgtact cgacggacgc cagccccagt tcggctgcga catcgacgga
     1381 atcgccgccc agtgcacggt cgataccgga gcgcgcgatt cgatcacgct gatgggcccg
     1441 ttcatggccg cttatccgca agtggtcccg aaaacgttga ccgcggccgg cgtg
//
LOCUS       DASUCJ010001141         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1418382, whole genome shotgun sequence.
ACCESSION   DASUCJ010001141 DASUCJ010000000
VERSION     DASUCJ010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418382"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1125)
                     /locus_tag="VFU90_13395"
     CDS             complement(190..1125)
                     /locus_tag="VFU90_13395"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5480833.1"
                     /translation="MELAVFCGSANEPLGRAIAEGMGARLGRRTAARFPDGESHVEVL
                     DSVRGRDVYLVQPTSPPTDENLMELLLLADACRRAGAARITAIVPYLGYARQDRRSKG
                     REGVGARLVADMLATAGIERIVCVDLHSSSIEGMSSVPIEHVSAARLIADAVAPLASK
                     RSVVVAPDLGAVKLAERYARYLDLPLAVVHKVRLSGEEVEVRGIIGDVAGLSPVIVDD
                     MITTGVTIEAAVGALLRAGCNPAPIVAATHALLVGDARRRLRELSVTCIATTDSIAAG
                     PDDTLPMRVIGLGALLSVVAVRLHENKSLADYLGR"
     gene            1061..>1241
                     /locus_tag="VFU90_13400"
     CDS             1061..>1241
                     /locus_tag="VFU90_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480834.1"
                     /translation="MPSAIARPSGSFAEPQKTANSISGLRGVRAALSRAWDVTALTRA
                     FTTANTLDSTIFTAKV"
BASE COUNT          226 a          422 c          380 g          213 t
ORIGIN      
        1 tcccgaacag gtcgctgcgg atcggctcct cgatctcgcg agggacggcc ggccgtctac
       61 ggtcgagcgg acgcgctggg ttcaagtctg cctttcgtca tcggccgatg agccgtgctt
      121 gcgccgttac ctgcttcgta cgtcgcactc gcccgcctcc tgcgatgcta gccagccttc
      181 cgccggctct caacggccca gatagtccgc gagcgactta ttctcgtgaa gacgaacggc
      241 gacgaccgat agcaatgcgc cgagaccgat gactctcatg ggcaacgtgt catctggacc
      301 ggcggcaatg ctgtccgttg tcgcgatgca tgtcaccgac agctcgcgaa ggcgtctccg
      361 agcatcgccg acgagtaacg catgagtggc cgcgacgatg ggagcgggat tgcaacccgc
      421 ccgaaggagt gcaccgaccg cggcttcgat cgttacgccc gtcgtgatca tgtcgtcgac
      481 gatgaccggg gataagccgg cgacgtcgcc gatgattccg cgaacctcca cctcttcgcc
      541 agataagcgg accttgtgca cgacggccaa gggtaggtcg agatagcgcg cataacgttc
      601 ggcgagcttc accgcgccca agtccggtgc gaccacgacg ctgcgcttag aagcgagcgg
      661 cgcgacggca tctgcgatca ggcgcgcagc gctcacgtgc tcgatcggca ccgaggacat
      721 tccctcgata gaagagctat gcagatccac acagacgatt cgctcgatgc cggccgtcgc
      781 gagcatgtcg gcgacaagtc tagcgcctac gccttctctt cccttcgagc ggcggtcttg
      841 gcgcgcgtaa ccgaggtacg gaacgatcgc cgtgatgcga gcggcgcctg ctcggcgaca
      901 cgcatccgca agcagcagca actccatgag gttctcgtcg gtcggcggcg acgtgggttg
      961 caccagatac acgtcgcgcc cgcggacgct atcgaggact tcgacatgcg actcgccgtc
     1021 cgggaatcgc gccgccgtgc gtcgtcccag gcgggcgccc atgccttccg cgatcgcacg
     1081 tccaagcggc tcgttcgcgg agccgcaaaa gacggctaac tccatctcgg gccttcgcgg
     1141 cgtccgcgca gcgctctcac gcgcatggga tgtgaccgca ctgacacgcg ccttcaccac
     1201 ggccaacacc ctcgattcga caatattcac tgcaaaagtc c
//
LOCUS       DASUCJ010001142         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_394174, whole genome shotgun sequence.
ACCESSION   DASUCJ010001142 DASUCJ010000000
VERSION     DASUCJ010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394174"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFU90_13405"
     CDS             complement(<1..320)
                     /locus_tag="VFU90_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480835.1"
                     /translation="MEVERALADLAEVRDRLAACQQFKGYSGPAAAASGAIAIVAGIA
                     QFALVPHPVTAADERMYLYVWFSCLAAALVLNYGSIGVWYARTGAAQERVRARSAGVA
                     LLPA"
     gene            531..>1006
                     /locus_tag="VFU90_13410"
     CDS             531..>1006
                     /locus_tag="VFU90_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5480836.1"
                     /translation="MELGDIQVYHHGDFVKYGDAKVGLLTHGLNYGTGCFEGIRGYWN
                     ADHAEVYLFRLAEHFERLHLSAKLLLMDLDDDVATMCEATTKLIRLNDYRQDVYVRPI
                     AFKAAEEIGVRLHNVKDDFAIVAIPHRSYFETGAGLRTAVSSWRRIDDNSAPARAK"
BASE COUNT          200 a          331 c          312 g          163 t
ORIGIN      
        1 atggccggca gcagcgcgac gccggcgctt cgagcacgca cgcgttcttg cgccgcgccg
       61 gtgcgtgcgt accagacgcc gatcgagccg tagttcaaca cgagcgcagc cgcgaggcac
      121 gagaaccaca cgtagaggta catgcgttcg tcggccgccg tcaccggatg cggcacgaga
      181 gcgaactgcg cgatgcctgc gacgatcgcg atggcgccgc tcgcagcggc cgccggacct
      241 gaatacccct tgaactgctg acaggccgcg aggcgatctc tgacctcggc gagatcggcc
      301 agcgcgcgtt ctacttccat atgcactttg tatcacaaag tgcaaggggg cgcaatcccc
      361 cggaggtctc gttcgtggcg ggacgctagt tcatggtgcc gacacttggc ccaagacatg
      421 cgcgcaccgt gttccgtgcg tcctgcaggc gggcaaggtg aggaaaggca gccgtctgaa
      481 agcaccgctc atctcgtcat cgacctcttc ccagatcgga gagagaccgc atggaactcg
      541 gcgacatcca agtctaccac cacggagact tcgtcaagta cggcgacgcg aaggtcggct
      601 tgctcacgca cgggctcaac tacggcaccg gctgtttcga aggcatccgc ggctactgga
      661 acgccgacca tgcggaagtg tatctcttca ggctcgccga gcacttcgag cggctccatc
      721 tctcggcgaa gctgctcctc atggatctcg atgacgacgt cgcgacgatg tgcgaggcga
      781 cgacgaagct catccggctc aacgactacc ggcaggacgt ctacgtcaga ccgatcgctt
      841 tcaaagccgc cgaagagatc ggcgtgcggc tgcacaacgt caaagacgat ttcgcgatcg
      901 tcgcgatccc gcatcgctcg tacttcgaga ccggagccgg gctgaggacc gcggtctcat
      961 cgtggcggcg catcgacgac aacagcgccc ccgcccgggc gaaaat
//
LOCUS       DASUCJ010001143         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_837489, whole genome shotgun sequence.
ACCESSION   DASUCJ010001143 DASUCJ010000000
VERSION     DASUCJ010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837489"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFU90_13415"
     CDS             complement(<1..344)
                     /locus_tag="VFU90_13415"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU5480837.1"
                     /translation="MPWLIAAYIFAATFAWFTRTFTPHHHWTYPVFRQSFYHLMAHRN
                     LYASDPAQGYDFFKYSPTFALLFAPFALLPSSVGLLVWDITNAVALLAAVAMVLPRRQ
                     AAIAAIVVLPEV"
     gene            489..821
                     /locus_tag="VFU90_13420"
     CDS             489..821
                     /locus_tag="VFU90_13420"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU5480838.1"
                     /translation="MATVDDFLTLDIRVGTVVAAEPFPEARKPAIKLRIDFGPALGVK
                     RSSAQLTVHYTPEQLVGRQVVAVVNFPSRRIAGFESDVLVLGAMPTEREVILLCVDRA
                     VDNGTRIG"
     gene            1120..>1466
                     /locus_tag="VFU90_13425"
     CDS             1120..>1466
                     /locus_tag="VFU90_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5480839.1"
                     /translation="MATATTPAAAPDKTILAKGYTHPELLVSTDWLAANLDNPSIRII
                     ESDEDVLLYDMGHIPNAQKVDWHADLNDPIVRDYVDREHFQELLRSRGIDDSKTVVFY
                     GDKNNWWACYALWV"
BASE COUNT          291 a          490 c          430 g          255 t
ORIGIN      
        1 aacacttccg gcagcaccac gatcgcggca atcgcggcct ggcgtcgcgg cagcaccatc
       61 gccaccgcgg cgagcaatgc taccgcattc gtgatgtccc agacgagcag cccaaccgac
      121 gacggcagca gcgcgaacgg cgcgaacaag agcgcgaacg tcggactgta cttgaagaaa
      181 tcgtatccct gcgccggatc gctcgcgtac agattgcgat gcgccatcaa gtgatagaac
      241 gattgccgaa agacgggata cgtccagtga tggtgcggcg tgaacgtccg cgtgaaccac
      301 gcgaacgtgg cagcgaagat gtacgccgcg atgagccatg gcattgcgcg atccaaccac
      361 gcggcaccgt ccgtctcgcc aacgaaagat tcgctcgccg ccatcggtct gtgattggac
      421 attattgcat gccgcaccgc atgcagccgg ggttgccgcg aacgtcacgc ggcgcgaggt
      481 tgtccgccat ggccaccgtc gatgacttcc tcacgctcga catccgcgtc ggcaccgtcg
      541 tcgcggccga gccgtttccc gaagcgcgaa aacccgcgat caaactgcgc atcgatttcg
      601 gacccgcgtt gggcgtgaaa cgatcgagcg cccaactcac ggtccattat acaccggagc
      661 aactcgtggg acggcaggtc gtggccgtcg tgaattttcc ttctcgtcgc atcgcgggct
      721 tcgagagcga cgtgctggtg ctcggcgcca tgcccacgga gcgcgaggtc attctgctct
      781 gtgtcgatcg cgccgtcgac aacggcacgc gtatcggcta gcaccgtact cgaaccccgg
      841 acatccttga cacggacgcg gctgccggat acttgttgac tcatgatgat ggcgcagtcg
      901 cgtaaggatc aatgcatgtg cgcgtgttgc cgggccgcct gtcggtggcc gggcggatcc
      961 gcgcgtcgtc gctgctgaca tcatcgtcgc aacgcgcatt ccggccggtc gctgaaagca
     1021 gcggccggct tttttgtctc cgtcgagccc gacgcgaacc atttccagtg cgtcggcgtt
     1081 cgtcacctgc acctctcact cgagcacgag gaccgaccca tggcaaccgc caccacaccc
     1141 gccgccgcgc ccgacaagac gattctggcc aagggataca cccatccgga actactcgtc
     1201 tccaccgatt ggttggcggc caatctcgac aatccatcca tccgcatcat cgagagcgac
     1261 gaagacgtgc tgctctacga catgggccac attccgaacg cacagaaagt cgattggcac
     1321 gccgacctca acgatccgat cgtgcgcgac tacgtcgacc gcgagcactt ccaggaactg
     1381 ctccgctcgc gcggcatcga cgattcgaaa acggtcgtct tctacggcga caagaacaac
     1441 tggtgggcct gctacgcgct ctgggt
//
LOCUS       DASUCJ010001144         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_272077, whole genome shotgun sequence.
ACCESSION   DASUCJ010001144 DASUCJ010000000
VERSION     DASUCJ010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272077"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFU90_13430"
     CDS             <1..201
                     /locus_tag="VFU90_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480840.1"
                     /translation="LGNVTVPNPVWSFVQGCSPPTGTSTYNNQPGCQVAVTVQYPFSF
                     FMPFMPGTSIMLSSTSKMLISF"
     gene            336..>1305
                     /locus_tag="VFU90_13435"
     CDS             336..>1305
                     /locus_tag="VFU90_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480841.1"
                     /translation="MESYDRKHRFAIYTLRVSNHLKTPLFCRIWVISHDGAAQSAHQT
                     IIEIAPLSERSTDVPIWMDDCNSFERALAEIVGEGVECIVEAAAPQTETVAALPAAAE
                     IARDGRFAIAGVALAAIVVAAVVFSMAVPRIAAFAAPPMALNGTTVRAEYSASGAGKI
                     AYAVLAPDGHTVQSGPLKDYEGAIPIAIPPSSVNGAFTLQLTMRGPLGSAKEIRVLNT
                     VPRANDAAQISDISVDPPVAKPGQTVTVTYAAAGTDGYVRLLGTDGTIWGQQPFLRSG
                     KASFVVPALNDSREMHVLLHVTKGQSVAQSTAGLVVAGAPAPAPRPA"
BASE COUNT          216 a          452 c          402 g          235 t
ORIGIN      
        1 ttgggcaacg tgaccgtccc caatccggtc tggtcgttcg tccaggggtg ctcgccaccg
       61 accggaacga gtacgtacaa caatcagccc ggctgccaag tggcggtgac cgttcagtat
      121 ccgtttagtt tcttcatgcc gttcatgccg ggcacgtcga tcatgctgtc gagcacctcg
      181 aagatgctca tctccttcta gccgtgcggt aaagcaatat tttgaattcc gcacttgcgt
      241 tgcgcaccgg gtccgctccg ctagcgccgt cacgaaaacc aggttccgga gccgccgtca
      301 aaacggcggc cggcggtgtt gcggccgctt tcgtcttgga aagctacgac cgtaaacatc
      361 gcttcgcgat ttatacgtta cgcgtttcca accacttgaa gacgccgctc ttctgccgca
      421 tctgggtgat ctcgcacgac ggcgccgccc agtcggccca tcagacgatc atcgagatcg
      481 cgccgctctc ggaacgctcg accgacgtac cgatctggat ggacgattgc aattcgttcg
      541 agcgcgcgct cgccgagatc gtcggggagg gcgtcgagtg catcgtcgaa gcggccgcgc
      601 cgcagaccga aacggtcgcc gcacttccgg ccgccgccga aatcgcgcgc gacggccggt
      661 tcgcgatcgc cggcgtcgcg ctcgccgcga tcgtcgtggc ggcggtcgtt ttctcgatgg
      721 ccgttccgcg catcgcggcg ttcgccgcgc cgccgatggc gttgaacggc accaccgtcc
      781 gcgccgaata cagcgcttca ggcgccggga agatcgccta tgcggtgctc gcacccgacg
      841 gccataccgt ccaatccggc ccgttgaaag attacgaggg tgcgattccg attgcgattc
      901 caccttcgag cgtcaacggc gcattcacgc tgcagctcac gatgcggggg ccattgggca
      961 gcgccaagga gattcgcgtg ctcaataccg tcccgcgcgc caacgacgcc gcgcaaatca
     1021 gcgacatctc ggtcgacccg ccggtcgcaa agccgggcca aaccgtgacg gttacctacg
     1081 ccgccgccgg gaccgacggc tacgtccggc tgctcggcac cgacggcacg atttgggggc
     1141 agcagccgtt cttgcgttcg ggcaaagcct cgttcgtcgt cccagcgctc aacgacagcc
     1201 gcgagatgca cgtgctgctg cacgtcacca aggggcagtc ggttgcgcag tccaccgccg
     1261 gactggttgt cgccggcgcg cccgcgccgg ctccgcgccc ggccg
//
LOCUS       DASUCJ010001145         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_27528, whole genome shotgun sequence.
ACCESSION   DASUCJ010001145 DASUCJ010000000
VERSION     DASUCJ010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27528"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFU90_13440"
     CDS             <1..422
                     /locus_tag="VFU90_13440"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU5480842.1"
                     /translation="HEVVKAALLQRWYRAAPQGFGLVPETTFRLSEDTYLEPDVVIYP
                     RASGIRGLTGANVLLVVEIADSSLRYDIGRKAALYASFGVRELWAIDAARLTARVFRD
                     PAADGFRDARDFDSADRLTPLFAPEQFALRLDELELS"
     gene            complement(483..>1097)
                     /locus_tag="VFU90_13445"
     CDS             complement(483..>1097)
                     /locus_tag="VFU90_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5480843.1"
                     /translation="NGAGKSTLIKVLSGVELPDGGEIRVEGRPVAIRSPRSAMQLGIE
                     TVYQYNSLVPNMSVARNLFIGREPLRFSVGGVGVMNQRKMRRDSTRAIVDVDLHLRSP
                     DTLVAELSGGQRQGVAIARAMYFKSKVLILDEPTNHLSVRETAKVLGFVKGLAAQKVT
                     TILISHNLHHVFVSCERVVAMARGKVVLDKSVAETSIDEIVDVI"
BASE COUNT          197 a          366 c          328 g          206 t
ORIGIN      
        1 atcacgaagt cgtgaaggcc gcgcttctcc aacggtggta tcgcgccgcg ccgcaggggt
       61 tcggcttggt gcccgaaact actttccgcc tcagcgagga cacctatctc gagccggatg
      121 tcgtcatcta tccgcgcgcg agcggcatcc gcgggttgac aggcgcgaat gttctgctgg
      181 tcgtcgagat cgccgactcg tcgctccgct acgacatcgg ccgcaaggcg gcgctctacg
      241 ccagcttcgg cgttcgcgag ctgtgggcca tcgacgcggc tcggctgacc gcgcgcgtct
      301 tccgcgatcc ggcggcggac ggttttcgcg acgcgcgcga ctttgactcc gccgaccggc
      361 tcacgccgct cttcgcgccg gaacaattcg cgctgaggct ggacgaactg gagctatcgt
      421 aacagcccgc gtctacgccc gcgcgattcg cgccccgcgg gctgcatggc cgcaactccg
      481 gcctagatca cgtccacgat ctcatcgatc gacgtctcgg cgacactctt gtcgagcaca
      541 accttgcctc tcgccatcgc gacgactcgt tcgcaggaga cgaatacatg gtggagattg
      601 tggctgatga gaatcgtcgt caccttctgc gccgcgaggc ctttgacgaa gccaagcacc
      661 ttcgctgtct ccctgaccga aagatggttc gtgggctcgt cgagaatcag gaccttcgac
      721 ttgaaataca tcgcgcgggc gatcgcgacg ccttgacgct gtccgccgga aagctcggcg
      781 accaaggtgt ccggcgaccg cagatggaga tcgacgtcga caatagctcg cgttgaatcc
      841 cggcgcatct ttcgctgatt catgacgccg acgccgccca cggaaaacct cagcggttcg
      901 cgaccgatga agagattgcg agcgaccgac atattcggga ccagagaatt gtattggtag
      961 accgtctcga tccccagctg catcgccgac ctcggcgatc ggatcgcaac cggccgacct
     1021 tcgacgcgaa tctcgccgcc atcgggaagc tcgaccccgg aaagcacttt gatgagcgtc
     1081 gatttgccgg ccccatt
//
LOCUS       DASUCJ010001146         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_363986, whole genome shotgun sequence.
ACCESSION   DASUCJ010001146 DASUCJ010000000
VERSION     DASUCJ010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363986"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFU90_13450"
     CDS             complement(<1..1082)
                     /locus_tag="VFU90_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5480844.1"
                     /translation="MTDPTQRPDNGVVLDSVDPSSIKRIHLVGVAGTGMGSFAGMLGA
                     AGYQVTGSDANVYPPMSDMLRAWGIDVMTPYAAENLDRARPDLVIIGNVIRRENPEAT
                     AVRERRIPQMSFPAAFGSLVLAGKHSVVIVGTHGKTTTSALMAHVLVAAATDASFLVG
                     GVTVNYNGNFRYGQGPYVVVEGDEYDTAYFDKGPKFLHYRARTAILTSIEFDHADIYR
                     DMAHYESAYVRFAETLPADGALAVCAAYPNAVEIARRYSRARVETYGAGDEIEADYAA
                     HDATFEPEGAHFTMREPDGAVSRMLLPMAGRHNVENAAGVYAAARSLGLTPLRIAEGF
                     TSFRGVKRRQEERGEPNGVLVIDDFA"
     gene            complement(1146..>1570)
                     /locus_tag="VFU90_13455"
     CDS             complement(1146..>1570)
                     /locus_tag="VFU90_13455"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480845.1"
                     /translation="HTTVTIGYDTPWRQVHELLIEAARRTENVLPDPAPFVLQTSLDD
                     NFVTYEINAYTDKPNEIATTYSLLHANIQNTFNEAAVEIMSPHYTSLRDGNRSTVPAS
                     HLDARYTAPAFRVALDGNPHPRTFTGTTTGVASAEPRG"
BASE COUNT          244 a          488 c          547 g          291 t
ORIGIN      
        1 tgcgcgaagt catcgatgac gaggacgccg ttcggctcgc cgcgctcctc ctggcgtcgc
       61 ttcacgccgc ggaaggacgt gaacccttcg gcgatccgga gcggcgtcag gcctaacgag
      121 cgtgcggccg cgtagacacc cgccgcgttc tcgacgttgt ggcgcccggc catggggagc
      181 agcatgcgag agaccgcgcc gtccggctcg cgcatcgtga agtgcgcgcc ttccggctcg
      241 aatgtcgcgt cgtgcgccgc gtagtcggcc tcgatctcgt caccggcgcc gtaggtctcg
      301 acccgcgcgc gcgagtaccg gcgcgcgatc tccaccgcgt tcgggtacgc cgcgcacacg
      361 gcgagcgcgc cgtccgccgg aagcgtctcg gcgaagcgca cgtaggcgga ctcgtaatgc
      421 gccatgtcgc ggtagatgtc ggcgtggtcg aattcgatgc tggtgaggat cgccgtacgg
      481 gcacgatagt gcaggaactt cggtcccttg tcgaagtagg cggtgtcgta ctcgtccccc
      541 tcgacgacga cgtagggtcc ctggccatat cggaagttgc cgttgtaatt gacggtgacg
      601 ccgccgacga gaaacgatgc gtcggttgcg gcggcaacca gcacgtgcgc catcaatgcc
      661 gacgtcgtcg ttttcccgtg cgtgccgacg atcacgacac tgtgcttccc ggcgagaacg
      721 agcgacccga aggccgcggg gaagctcatc tgcggaatcc gccgctcacg gacggctgtc
      781 gcttcgggat tctcgcggcg aatcacattc ccgatgatga cgagatccgg tcgcgcgcgg
      841 tcgaggttct cggcagcgta gggcgtcatg acgtcgattc cccacgcgcg gagcatgtcg
      901 ctcatcggtg ggtagacgtt cgcgtcgctt ccggtcacct ggtaacccgc tgccccgagc
      961 atgcccgcga acgagcccat gccggtgccg gcgacgccca cgaggtggat gcgtttgatc
     1021 gagctcgggt cgacggaatc gaggacgacg ccgttgtccg gccgctgtgt gggatctgtc
     1081 atacgaccgg gaaggttagt cgctgacttg gcgcccgggc tagtgcgcgc cggagagcat
     1141 ctccctcacc ccctcggctc cgccgaggct actccggtgg tcgtgccagt gaacgtccgt
     1201 ggatgtggat tgccgtcaag ggcgacccga aatgccggcg ccgtgtaccg cgcgtcgaga
     1261 tgcgacgccg ggacggtgct gcgattgcca tcgcgcagag acgtgtagtg tggggacatg
     1321 atctcgaccg cggcttcgtt gaacgtgttc tgaatgttgg catgcaggag tgaataggtg
     1381 gtcgcgatct cgttgggctt atccgtgtag gcattgatct cgtaggtgac gaaattgtca
     1441 tcgaggctcg tctgcagcac gaatggcgcg ggatcgggga gcacgttctc ggtccggcgc
     1501 gcggcctcga tgagcagctc gtgcacctgg cgccacggcg tgtcgtaacc gatggtgacc
     1561 gtcgtgtgga
//
LOCUS       DASUCJ010001147         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_211244, whole genome shotgun sequence.
ACCESSION   DASUCJ010001147 DASUCJ010000000
VERSION     DASUCJ010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211244"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFU90_13460"
     CDS             complement(<1..194)
                     /locus_tag="VFU90_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480846.1"
                     /translation="MATTIVTTAGTTARHTTDAHPVGRERTGAGTSLLIGSLLLAALV
                     LRVHGLGKANLWLDEANSWYV"
     gene            217..711
                     /locus_tag="VFU90_13465"
     CDS             217..711
                     /locus_tag="VFU90_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480847.1"
                     /translation="MLTRHASSSDKNGVTRIAIATKDSAFNVVPPSRGGASGSVWFKS
                     RAPSLALQLRAAGLVPSKRYQLELSTDQAVYTVARLTSDAQGRLAFDTTLTQLADDAC
                     VVANRVPPLPLRGRVKIKFWVRRDASPTVAGSACSGNGDGDSTYALLEENVGTFYGTP
                     HERR"
     gene            complement(708..1841)
                     /locus_tag="VFU90_13470"
     CDS             complement(708..1841)
                     /locus_tag="VFU90_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEU5480848.1"
                     /translation="MPIRTLSFTRGLWRIASAGASAGNRVELLRDGGQAFESMLGAIA
                     DARDSVALECYLVRADSVGNRFADALIAAAKRGVRVRMVADWVGSRGASRAYWKRLRD
                     ADVHVRMFNPPGIRPWLGVLPRDHRKVLVADDVVGITGGFGLSEVWSGIMRRHRTAPW
                     RDTAVRIEGPAAVDMSRAFQVMWRRANFEQRPHRWRRRQLVRPARGADTDPQTAQGAV
                     VGIVEGEPWRLRVARALQLQAASAEKFIWIASAYFYPSAAELEALAGAAQDGVDVRLL
                     VPSKSDHPWVRRLTRRVYHRLLRNGVRIWEWNGAMMHAKTSVIDGHWVRVGSTDFNPL
                     GIAINYELDALIEDAALGEAAVEMYLADLASSTEIRWRGRADR"
BASE COUNT          347 a          753 c          689 g          321 t
ORIGIN      
        1 acgtaccatg agttcgcttc gtcgagccag aggttggcct tgcctaaccc atgcacccgc
       61 aacacgaggg cggccagcag gagagatccg atgagcaacg acgtgccggc gcccgtccgt
      121 tcgcgcccca ccgggtgggc atccgtggtg tggcgtgcgg tggtgccggc agtcgtcacg
      181 atcgtcgttg ccatgttcct ctatggaccg aaaggattgc tgacgcggca cgcctcgtcg
      241 tcggacaaga acggcgtgac gcggattgcc atcgcgacca aggacagcgc gttcaacgtg
      301 gtccctcctt cgcgcggcgg cgcgagcggc tcggtgtggt tcaagtcgcg tgcgccgtcg
      361 ctcgcgctac agttgcgcgc tgcggggctc gtgccatcga agcgctacca actcgagctc
      421 tccacggatc aggccgtcta cacggtggcg aggctgacct cggatgccca gggtcgcctg
      481 gcgttcgaca cgacgctgac gcagctcgcg gacgacgcct gcgtcgtcgc gaatcgtgtg
      541 ccgccgctcc cgttgcgggg gcgcgtgaag atcaagttct gggtgcgtcg cgacgcgagt
      601 ccgacggtgg cgggcagcgc gtgttcgggc aacggcgacg gggactcgac gtacgcgctg
      661 ctcgaagaga acgtcgggac cttttacggg acgccgcatg agcgtcgcta acggtcggcg
      721 cgcccccgcc accggatctc ggtggatgat gccaggtcgg ccaggtacat ctcgactgcc
      781 gcttcgccta acgctgcgtc ctcgatgagc gcgtcgagct cgtagttgat cgcgatgccg
      841 agcgggttga agtcggtcga ccccacgcgg acccagtggc catcgatcac actcgtcttg
      901 gcgtgcatca tcgcgccgtt ccactcccag atgcgcacgc cgttgcgcag caggcggtga
      961 tacacgcgtc gcgtgaggcg ccggacccac gggtggtcgg acttgctcgg caccagcaag
     1021 cgaacatcca cgccatcctg cgccgcgccg gccaacgctt cgagctcggc cgccgacgga
     1081 tagaagtagg cgctcgcgat ccagatgaac ttctcggccg acgcggcttg cagctgcagc
     1141 gcgcgcgcca cgcgcaatcg ccacggctcg ccctcgacga tgcccaccac cgccccttgc
     1201 gccgtctgcg gatcggtatc ggcgccgcgc gccgggcgca cgagctgccg gcgacgccag
     1261 cggtggggac gctgctcgaa attggcccgc cgccacatca cctggaacgc acgcgacatg
     1321 tccactgccg cgggtccctc gatgcggacc gccgtgtcgc gccacggcgc cgtgcgatgc
     1381 cggcgcatga tgccggacca tacttcgctc agcccgaagc cgccggtgat gccgacgaca
     1441 tcgtcggcga ccaggacctt gcggtggtcg cgcggcagga cgcctaacca gggacggatc
     1501 ccgggcggat tgaacatgcg cacgtgcacg tcggcgtcgc gcagccgctt ccagtacgca
     1561 cgacttgccc cgcggctccc cacccaatcg gccaccatgc gcacgcgcac gccgcgcttc
     1621 gcggcggcga tcagcgcatc ggcaaaccgg ttgccgacgg aatcggcgcg caccagataa
     1681 cattccagcg ccacgctgtc gcgcgcgtcg gcaatcgccc cgagcatcga ctcgaacgcc
     1741 tgcccgccgt cgcgcagcag ctccacgcga ttgcccgcgg acgcaccagc cgacgcgatc
     1801 cgccacaacc cgcgcgtgaa cgacagcgtc cggatgggca tcggcaccgg ggccgccgat
     1861 tcgatcggcg tgacgtccac agacagtggt gaaggtgagc tcatgactgg aaccggccgg
     1921 tggtatcgcg tataaggtag tgtcgctagc cagaaccggg ccagcgaccc gtcactttct
     1981 ttcgtcgaac gcactcacgc acaatgccag acgcaccgac aggaacgtat ccgcgctccc
     2041 cgctcgatct cgacgccgac gccatgcggc gactcggcta ccgcgtcacc gacaccatcg
     2101 ccgagcatct
//
LOCUS       DASUCJ010001148         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_669365, whole genome shotgun sequence.
ACCESSION   DASUCJ010001148 DASUCJ010000000
VERSION     DASUCJ010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669365"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VFU90_13475"
     CDS             <1..1172
                     /locus_tag="VFU90_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480849.1"
                     /translation="GINPGPLGTPHLDLPPWRGRILPPVRPGPDGHFDHIPIGTPEFA
                     EAHVFGTIRFTLDVWERYFGRRIKWHFARDFDRLEVVMLPDFDNSHAGWGFMEVGSYH
                     ETDDGTPVPFALNFDVIAHETGHLIIYSTLGVPSTATERGEYFGFQESAADTTAMISS
                     LHFGSLVERLLEETHGNLFTFNELDRFAEVSATEQIRLASNSVKLSQFAAGWDDEHAL
                     SQPLTGALFDILVDVFQENLVERGLIGRHVADLNDLVRNYPEYATTIQPAFDSAYARR
                     RDEFRMALIEARDYLGVALAETWKRLSADYFGYDEVGTLLVAVDRALTGGRYRNEIVE
                     SFVWREIGAIKVGPRLSRPDKSSHAHSARTLVPEMARRLPKISYRERALLARAAR"
BASE COUNT          213 a          389 c          372 g          225 t
ORIGIN      
        1 atggcatcaa tcctggtccc cttggcacgc cccatctcga cttgccgcca tggcggggac
       61 gaatacttcc accggtgcga ccgggccccg atggccactt cgatcatatt cccatcggca
      121 cgccggagtt tgccgaggcg cacgttttcg gcaccatccg gttcactctc gacgtgtggg
      181 agcgctattt cggtcggcgg atcaagtggc acttcgcgcg cgacttcgac aggctcgagg
      241 ttgtgatgct gccggacttc gacaattccc atgcgggctg gggcttcatg gaggtcgggt
      301 cctatcacga gaccgacgac ggcaccccgg ttcccttcgc gctcaatttc gacgtcatcg
      361 cgcacgagac cggacatctc atcatctaca gcacgctggg cgtgcccagc accgcgaccg
      421 aacggggcga gtacttcggg tttcaggagt cagccgccga cacgacggcg atgatctcgt
      481 cgcttcattt cggttcgctc gtcgagcgcc tgctggagga gacgcacggg aatctcttca
      541 ccttcaacga gctcgaccgc ttcgccgaag tgtcggcaac cgagcagatc cgtctcgcca
      601 gcaattcggt aaagctgtcg caattcgccg cgggatggga cgacgagcac gcgttatcgc
      661 agccgctcac cggtgccctg ttcgacatac tcgtcgacgt gttccaggaa aatctcgtcg
      721 agcgcggcct gattgggcgg catgtcgcgg acctcaatga tctcgtcagg aactatccgg
      781 aatacgccac gacaatccag ccggcgttcg attccgccta tgcgcggcga cgcgacgaat
      841 tccgcatggc gttgatcgag gctcgcgact atcttggcgt cgcgctcgcc gagacctgga
      901 aacggctgtc ggcggactac ttcgggtacg acgaggtcgg gactctcctg gtcgcggtcg
      961 atcgcgccct gaccggcgga cggtaccgaa acgaaatcgt cgagagtttc gtctggcgcg
     1021 agatcggcgc gatcaaggtc gggccgcgtc tatcgcggcc ggacaagtcg agccatgccc
     1081 actcggcgcg cacgttggtg ccggagatgg cacgccgcct gccgaagatt tcctaccgcg
     1141 aacgcgcgct gctcgcgcgc gccgcgcgat gatgccacgg caaaacggag ggaccaatg
//
LOCUS       DASUCJ010001149         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_58420, whole genome shotgun sequence.
ACCESSION   DASUCJ010001149 DASUCJ010000000
VERSION     DASUCJ010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58420"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFU90_13480"
     CDS             complement(<1..224)
                     /locus_tag="VFU90_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480850.1"
                     /translation="MLSEDVAFDAKDRAVGLLGSVLDGAGGVAVSNDLHARMVCLGDV
                     IVCFDLGGVLIRICHSWEEACRRANLAPPRS"
     gene            397..936
                     /locus_tag="VFU90_13485"
     CDS             397..936
                     /locus_tag="VFU90_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5480851.1"
                     /translation="MASPSCCRAASGATKSTTRPSSPWSSAKRPKTSLRERALEYVLG
                     YTCANDVSARDWQKRYGGGQWCRGKTFDSFAPLGPRLVTTDEIANPNALRIATIVNGE
                     RLQDWTTADMIFDVPQLIAFLSGSTTLVPGTVILTGTPHGVGMARTPPRWLAAGDVVS
                     IEIEAIGTLTNPVVEESEG"
BASE COUNT          281 a          471 c          482 g          232 t
ORIGIN      
        1 gagcgcgggg gcgcgaggtt cgcgcggcga cacgcctcct cccagctatg gcatatccga
       61 atcagcacgc cgcccagatc gaagcagacg atcacgtcgc cgaggcatac catgcgggca
      121 tgaagatcgt tcgataccgc gaccccgccg gcgccatcca gtacggagcc gagcaggccg
      181 acggctctat ctttcgcatc gaaggcgacg tcttcggaga gcatcgggtc gtgccgtcgc
      241 ccgtaccggc gggcgccata cgcctcctcg cccccgtcgc gccgtcgcag atcctctgca
      301 tcgggctcaa ttatcgaaag cacgcggaag aaacgaacgc caagattccc gaatatccgg
      361 tgctctttct aaaaggcgtg aacgccgtcg cccaccctgg cgagcccatc gtgctgccgc
      421 gccgcctccg gagcgacgaa gtcgactacg aggccgagct cgccgtggtc atcggcaaaa
      481 cggccaaaaa cgtcccttcg ggagcgcgcc ctcgaatacg tgctgggtta cacgtgcgcg
      541 aacgacgtct cggcgcgcga ctggcaaaaa cgatatggcg gcggacaatg gtgccgcggc
      601 aagacgttcg attcgttcgc cccgctcggg ccgcgcctcg tcacgaccga cgagatcgcc
      661 aatccgaacg cgcttcggat cgcgacgatc gtgaacgggg agcgccttca agattggacg
      721 acggccgaca tgatcttcga cgtgccgcag ttgatcgcgt ttctcagcgg cagcaccact
      781 ctggtgccag gcacggtgat tctgacgggg acgccgcacg gcgtgggaat ggcgcgtacg
      841 ccgccgcgct ggctcgccgc gggcgacgtc gtgagcatcg agatcgaagc gatcgggacg
      901 ctgacgaatc cggtcgtcga ggagagcgaa ggctgacggc ggtgcgaaga tggcgggcac
      961 gacgttgccg gcgtgaattc gtggtgcgag ttggcgcccg ccgtgaccgg gctgccatgc
     1021 tgatggccat cgccgcacaa cggcctgcca tgctggcgcc catcgccgca caaggggcac
     1081 cgtggcgccg cagtcacgtc cgcgactcca agatcagggg caggcggtcc gaccggggtt
     1141 cggggacacg gccccgtttg tgcgcgtgcg ctcgtccgcc attgcggaaa ttccgcgcat
     1201 ctcgctcgcc gcggctattt tcccagggtc ttgagggtgc gaccccggag atgggcaaga
     1261 cgatttacaa ttgggatgaa gcgcaatctg ccgggcgcgc gcgacgttgc tgccgcagcg
     1321 aacgaactga cgcaccccga ggcggaatag ccccagaact ttgggaataa aattcgcaag
     1381 atcgtccggc gcgggcttgg agcccgaacc aaaggcgtga taagaccgcc cataattttt
     1441 tacggaaagg gaaggggctt taatca
//
LOCUS       DASUCJ010001150         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_944595, whole genome shotgun sequence.
ACCESSION   DASUCJ010001150 DASUCJ010000000
VERSION     DASUCJ010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944595"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFU90_13490"
     CDS             <1..187
                     /locus_tag="VFU90_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5480852.1"
                     /translation="EMDAACSCGEHAEILACIERGDGTGAARLIDSHIRHIESCLKLA
                     QSTPEAFDLRAAFAKAD"
     gene            complement(184..1407)
                     /locus_tag="VFU90_13495"
     CDS             complement(184..1407)
                     /locus_tag="VFU90_13495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEU5480853.1"
                     /translation="MPAIDGARLIRDLRCLAEFGCYKTGVHRPAFSAEDLAARRWLVG
                     RMAEAGLDAGIDGIGNVIGRAPGNGPRLLVGSHADTQPHGGWLDGAMGVVYGIELARA
                     LRDDSGCRGNGVDVAAWQDEESHYASFLGSRSFTGLLDEAEIDRARHRDDDTPLRDAL
                     ARAGLADAPRATLDPSRYRGYLEAHIEQGAVLETERRRVGVVTAIVGILVYRVTFVGA
                     QNHAGTTPMRLRRDAGVALTAFCNEVHQAFATRAGPSTVWTIGRIVLEPNAPSIVPGR
                     AEMLLQIRDPSLAVMEALGAVAIGLAGAADEAGPCRVEIACTSCTEPIAMDATFQDAI
                     ASAAARHVGQGWMRMHSGAGHDAQILARRMPAGMLFVPSIGGVSHHHTEDTDEDDIVL
                     GCQVFADAAEAILAL"
     gene            complement(1476..>2303)
                     /locus_tag="VFU90_13500"
     CDS             complement(1476..>2303)
                     /locus_tag="VFU90_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480854.1"
                     /translation="CDGTGGPMMLKRIVLAVGLCTACALSTASMATSYSTDQSDVWYN
                     PSESGWGFQLVQRGSVIFATMYLYGPDNAPVWYTALMYSSGTMIWEGDLYLTNGPWFG
                     TVPFDPGLVTYRKVGTMTWIPQSVDSGLISYTVDGVGVVKNVVREELVLDDFSGTYMG
                     GLHEESTACADPSLDQTTESSGLVQITQSGASIQMQSIGADGTIECVYSGTLTQAGQM
                     GGITGSFTCAGGSNGTLAASEMQVTPSGITGRFHKAYSNPPGCQGDGWFGGMRSTLH"
BASE COUNT          392 a          807 c          735 g          369 t
ORIGIN      
        1 ggagatggat gccgcctgct cgtgcggcga gcacgccgag attctcgcct gcatcgaacg
       61 cggcgatgga accggtgccg ccaggctgat agacagccac atccgccaca tcgagagctg
      121 cctcaagctc gcgcagtcga cgccggaggc gttcgacctg cgcgccgcgt tcgccaaagc
      181 tgactagagc gccaggatcg cttcggcggc gtccgcgaag acctggcatc cgagcacgat
      241 gtcgtcctcg tcggtgtcct cggtgtggtg gtggctgacg ccgccgatcg acggaacgaa
      301 cagcatgccc gcgggcatcc gtcgcgcgag aatctgcgcg tcgtgcccgg cgccgctgtg
      361 catgcgcatc cagccctgcc cgacgtgccg cgcggcggcg ctggcgatcg cgtcctggaa
      421 cgtcgcgtcc atcgcgatcg gctcggtgca tgacgtgcac gcgatctcaa cgcggcacgg
      481 gccggcctcg tccgccgccc cggcgagacc gatcgccacc gcgccgagcg cttccatcac
      541 cgcgagcgac ggatcgcgga tctgcagcag catctcggcc cgcccgggaa cgatgctcgg
      601 cgcattgggc tccagcacga tgcgtccgat cgtccacacg gtcgacggtc ccgcccgcgt
      661 ggcgaacgcc tgatgaactt cgttgcagaa cgcggtcagc gcgactcccg cgtccctgcg
      721 aagccgcatc ggcgtcgtac ccgcgtgatt ctgcgcgccg acgaacgtta cccggtagac
      781 gaggatgccg acgatcgcgg tgacgacgcc gacgcgtcgc cgctcggtct cgagcaccgc
      841 accttgctcg atgtgcgctt cgaggtagcc gcgatagcgc gagggatcga gcgtcgcgcg
      901 tggcgcatcg gcgagccccg cacgtgcaag cgcatcgcgc agcggagtat cgtcgtcgcg
      961 atgccgagcg cggtcgatct cggcctcgtc gagaaggccg gtaaagctgc ggctgccgag
     1021 gaagctcgcg taatgggatt cctcgtcctg ccacgccgcc acgtcgacgc cattgccgcg
     1081 gcatccggaa tcatcacgca gtgcgcgtgc gagctcgatg ccgtagacga ctcccatcgc
     1141 gccgtcgagc cagccgccgt gcggctgcgt gtcggcgtgc gagccgacga ggaggcgtgg
     1201 accgttgcca ggtgcgcggc cgatcacgtt gccgatgccg tcgatgccgg cgtcgaggcc
     1261 ggcttcggcc atgcgcccaa cgagccagcg gcgcgcggcg aggtcttctg cggagaacgc
     1321 cggccggtgc acgccggtct tgtagcaacc gaattcggca aggcaacgca ggtcgcggat
     1381 caggcgagcg ccgtcgatcg cgggcatatc gccacccctc tgcggccagg gacgctgggc
     1441 cggctgcgca cgaaatgcat tgccatcgcg tcgcttcagt gcagcgtgct tcgcatgccc
     1501 ccgaaccatc cgtcgccctg gcaacccggc ggattggaat acgctttgtg gaagcgaccg
     1561 gtgattccgc tcggcgtcac ctgcatctcg gaagccgcga gcgtaccgtt ggacccaccc
     1621 gcacaggtga acgagccggt gatgccaccc atctgccctg cctgcgtcaa cgttcccgaa
     1681 tagacgcact cgatcgttcc atcggcgccg atcgactgca tctggatcga cgcaccgctc
     1741 tgcgtgatct gcacaagtcc cgacgactcg gtcgtctgat cgagcgacgg atccgcgcag
     1801 gccgtagact cctcgtgcag accacccatg taggtaccgc tgaagtcgtc gaggaccagc
     1861 tcctcgcgca ccacgttctt gaccacgccg actccatcga ccgtgtagct tatgagcccc
     1921 gagtcgaccg attgcggaat ccacgtcatc gtgccgacct tccgatacgt cacgaggccg
     1981 ggatcgaagg gcaccgtgcc gaaccacggt ccgttggtca gatagagatc accctcccaa
     2041 atcatagtgc cgctcgaata catcagcgcc gtgtaccaga ccggcgcatt gtccggacca
     2101 tataagtaca tcgtggcgaa gatgaccgag ccgcgctgca cgagctggaa tccccacccg
     2161 gactcgctcg ggttgtacca gacgtcgctc tgatcggtcg agtacgacgt cgccatcgac
     2221 gccgtggaca acgcacatgc ggtacacaat ccgacggcaa gcacgatccg cttcagcatc
     2281 atgggccctc ctgtgccatc gca
//
LOCUS       DASUCJ010001151         1428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1051591, whole genome shotgun sequence.
ACCESSION   DASUCJ010001151 DASUCJ010000000
VERSION     DASUCJ010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051591"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFU90_13505"
     CDS             <1..1084
                     /locus_tag="VFU90_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5480855.1"
                     /translation="KEYASRGTWIWPPEPSLRLIADTIEFCAAEVPKFKAISVAGAHF
                     RDAGANAVQEMAFTLADGVTYCDTVVKRGRMTIDEFAPQISFFFYTHGDFFEEIAKYR
                     AGRRRWATIVRERYGAKSDKASMFRVGCVSGGASLYAPQAQNNVVRVAYEAMASVLGG
                     VQSMFTAAWDEPFALPSEESATLALRTQQILAYETGITKVADPLGGSYFIEALTDATE
                     ERIVEIMADLERHGGMVRAIEDGYLQGLISDEAFHIHQNIESGARPVVGLNKVVTDEP
                     PHDIGMYELDAEGRDLQLKRLNKVKSERDNGAAQEALAALSRAADGDNNLMHKLIDCA
                     NAYCTVGEMVSTLKTVWGEFQQPVVF"
     gene            1086..>1428
                     /locus_tag="VFU90_13510"
     CDS             1086..>1428
                     /locus_tag="VFU90_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5480856.1"
                     /translation="MTARVLVAKPGLDGHDRGAKIVARTLRDAGFEVIYTGIRQRIED
                     IVSIALQEDVALVGLSILSGAHLALTKRTIEALRAADAGDIPVVVGGTIPHADVPKLL
                     EAGAAAVFPTGT"
BASE COUNT          251 a          455 c          495 g          227 t
ORIGIN      
        1 caaggagtac gcctcgcgcg gcacgtggat ctggccgccg gagccgtcgc tgcggctgat
       61 cgccgacacg atcgagttct gcgccgccga ggtgccgaag ttcaaggcga tctcggtggc
      121 cggcgcccat ttccgcgacg ccggggccaa cgcggtgcag gagatggcgt tcacgctcgc
      181 cgacggcgtg acgtactgcg acacggtggt caagcgcggc cggatgacga tcgacgagtt
      241 cgccccgcag atctcgttct tcttctacac gcacggcgac ttcttcgagg agatcgcgaa
      301 gtaccgggcg ggtcgtcgtc gctgggcgac gatcgtgcgc gagcgctacg gcgcgaagtc
      361 cgacaaggcg tcgatgttcc gcgtcggctg cgtgtccgga ggcgcttcgc tgtacgcacc
      421 gcaggcgcag aacaacgtcg tgcgggtggc ctacgaggcg atggcgtcgg tgctcggcgg
      481 ggtgcaatcg atgttcaccg ctgcctggga cgagccgttc gcgttgccca gcgaggagtc
      541 ggccacgctg gcgctgcgca cccagcagat cctggcctac gagacgggca tcaccaaggt
      601 ggccgatccg ctcggcggct cgtacttcat cgaggcgctg accgacgcca ccgaggagcg
      661 catcgtcgag atcatggccg acctggagcg ccacggcggc atggtgcggg ccatcgagga
      721 cggctatctg cagggtttga tctccgatga ggcgttccat atccatcaga acatcgagtc
      781 tggggcccgc cccgtcgtcg gcctcaacaa ggtcgtcacg gacgaacccc cgcacgacat
      841 cgggatgtac gaactcgacg ccgaaggacg cgacctgcag ctcaagcgtc tgaacaaggt
      901 taagtccgaa cgcgacaacg gagccgcaca ggaagcgctc gcggcgctgt cgcgcgcggc
      961 agacggtgac aacaacctga tgcacaaact gatcgactgt gccaacgcgt attgcacggt
     1021 gggagagatg gtttcgacgc tcaagacggt ctggggcgag ttccagcagc cggtggtgtt
     1081 ctaaaatgac cgcacgagtc ttggtggcca agcccggcct cgacgggcac gaccgcggcg
     1141 cgaagatcgt ggcccgcacg ctgcgcgacg cggggttcga ggtcatctac accggcatcc
     1201 ggcagcgcat cgaagacatc gtgtcgatcg cgttgcagga agacgtcgcg ctcgtcggct
     1261 tgagcattct gtcgggcgcg cacctcgcgt tgacgaagcg caccatcgag gcgttacggg
     1321 ccgccgacgc cggcgacatc cctgtggtgg tgggcggcac gatcccgcat gcggacgtgc
     1381 cgaagttgtt ggaagccggc gccgccgccg tgttcccaac cggaacgc
//
LOCUS       DASUCJ010001152         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_654308, whole genome shotgun sequence.
ACCESSION   DASUCJ010001152 DASUCJ010000000
VERSION     DASUCJ010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654308"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="atpA"
                     /locus_tag="VFU90_13515"
     CDS             complement(<1..914)
                     /gene="atpA"
                     /locus_tag="VFU90_13515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5480857.1"
                     /translation="MINADEIAGILKQQIESFKTGLQEDEVGTVIEVGANLARVYGLR
                     GVRASELVEFPNGLQGIALNLEEDNVGVVIMGPDKDIKEGDKVRRTGRIASVPVGDAL
                     LGRVVNPLGQPIDGKGPIETNRYRTIENVAPGVVQRQSVKQPLQTGIRAIDALIPIGK
                     GQRELIIGDRSTGKTAIAIDTIINQKGRDVFCVYVAIGQKNSTVSALAQVLEQKGAMD
                     YTTIVAVSPSEAAALRWLAPFAGCAMAEELMYRGKDVLIIYDDLTKHAQSYREMSLLL
                     RRPPGREAYPGDVFYLHSRLLERAAKLS"
     gene            complement(926..1468)
                     /gene="atpH"
                     /locus_tag="VFU90_13520"
     CDS             complement(926..1468)
                     /gene="atpH"
                     /locus_tag="VFU90_13520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU5480858.1"
                     /translation="MADEKLAHRYAEAVFSLAAERNQVDRVHDDLAAIARVLEERPAF
                     KEFFTSPVVDRDAKERALSAAFSSRVSEIALHTLLLLVRKRRERLLDALLREYRKLQL
                     AARGAEPLTVTSARALSSAELSALVERLERLYGKKFEVTTVVDPSLIGGVRILMGDRR
                     IDGSVAGRLDALSRTLFATA"
     gene            complement(1461..1961)
                     /locus_tag="VFU90_13525"
     CDS             complement(1461..1961)
                     /locus_tag="VFU90_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480859.1"
                     /translation="MRPDAFYVQVAVWSQVVSAVLFMCVLAWLWFKYLQPAILAAQER
                     HNKQIAQAERHRDEAKATLEMLRNEIGGAERDAELIRTRAEAQAKREHEATVAETREA
                     GERAAHGAQAEFARALASARDRLRVELLDKALARAREDAAQRIDADANARLVDRFVAS
                     LEGTDG"
     gene            complement(1958..2386)
                     /locus_tag="VFU90_13530"
     CDS             complement(1958..2386)
                     /locus_tag="VFU90_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480860.1"
                     /translation="MFLQLDGTFWIQLVNFAIFFALLNVLFLRPVGQAIRKRRQYIDG
                     VVADYADYQAQAKELREQAERVRAQARREAEQKVAKARADASNESAELAARYAAKVAA
                     TMEEAARNADAELQRARAGEDRLVRQLAGQMVERTLGAAQ"
     gene            complement(2411..2662)
                     /gene="atpE"
                     /locus_tag="VFU90_13535"
     CDS             complement(2411..2662)
                     /gene="atpE"
                     /locus_tag="VFU90_13535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU5480861.1"
                     /translation="MLAFGIIVAGVAFGSAIGDGIVASKAVEAIARQPEARPNIFTFL
                     FLGVGVLEAFPIIALGLAFYMLIVVAGLPNGVFASLAGH"
     gene            complement(2720..3451)
                     /gene="atpB"
                     /locus_tag="VFU90_13540"
     CDS             complement(2720..3451)
                     /gene="atpB"
                     /locus_tag="VFU90_13540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5480862.1"
                     /translation="MQEKLGEHILWHLPVLGGVHADTIVTTWLVMVLSLVFFAWVGAS
                     YRSAYQSRRQVVVEGVVNYIADLATSTLGPKGEPFVPFFIALFVFIFLMNQFGMLPFK
                     AFGLPFGGSPTADLNTTVPLALLVFVLTWIAGLRMTGSARLKHLFKPFWALFPINVLE
                     ECARPATLAARLFFNIFVGELLFVIIASIVEAKVTIGPVNLSLAVTVLPFFIQFFNFF
                     VGTVQAFVFTLLAIVYLALSISDDH"
     gene            complement(3475..3891)
                     /locus_tag="VFU90_13545"
     CDS             complement(3475..3891)
                     /locus_tag="VFU90_13545"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU5480863.1"
                     /translation="MQDNPPTRRSYSDAEAAADLATYHTLKRSVSVRSLLVVAVVALV
                     FARWQPHPALALAAGGFCGVLNVLWTMRSGERLVESRFVGGFVLSSFVRIALFGIVPV
                     AFAALGPWWSMAWYFAGFFLPLGMFAYSARRAFTAK"
     gene            complement(3897..4082)
                     /locus_tag="VFU90_13550"
     CDS             complement(3897..4082)
                     /locus_tag="VFU90_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU5480864.1"
                     /translation="MNGVVPVLAAGGTFAIAVLAGLMAGIWLGRLTGHELWVAGGLFA
                     GMAVGGYSAVRLLLRSM"
     gene            complement(4079..>4494)
                     /locus_tag="VFU90_13555"
     CDS             complement(4079..>4494)
                     /locus_tag="VFU90_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEU5480865.1"
                     /translation="GDVPGVILVDPIDYQQMVFAVRGSTFVLTDSGGVQEEAPCLGKP
                     VLVMRDETERPEGLDAGTLELVGHRGERIFEAARRLLTDERYFGRMARAVNPYGDGHA
                     AERIAAWLLARLRGGPYPTPFAVAAASVGRPAATP"
BASE COUNT          833 a         1481 c         1440 g          740 t
ORIGIN      
        1 tcggagagtt tcgcggcgcg ttcgagcagc cgcgagtgca ggtagaagac gtcgcccggg
       61 taagcttcgc gccccggcgg acggcgcagc aggagcgaca tctcgcggta cgattgagcg
      121 tgcttggtga gatcgtcgta gatgatcagc acgtccttgc ctcggtacat cagttcttcg
      181 gccatcgcgc agccggcaaa cggcgcgagc caacgcagcg ccgccgcttc cgacggactc
      241 acggccacga tcgtggtgta gtccatcgcg cccttctgtt cgaggacctg cgcgagcgcc
      301 gaaacggtcg agttcttttg cccgatcgca acgtagacgc agaaaacgtc gcgtcctttt
      361 tgattgatga tggtgtcgat cgcaatcgca gtcttaccgg tcgagcgatc gccgatgatc
      421 agctcgcgct gacctttgcc gatcggaatc agcgcgtcga tcgcgcgaat accggtctgc
      481 agcggttgct tgaccgattg gcgttgcacc actcccgggg cgacgttttc gatcgtgcga
      541 tagcggttcg tctcgatcgg tcccttgccg tcgatgggct ggccgagcgg gttgacgacc
      601 cgtcccagca gcgcgtcgcc gaccggcacg gaggcgatcc gccccgtccg gcggaccttg
      661 tcgccctctt tgatgtcctt gtcgggcccc atgatcacga cgccgacgtt gtcctcttcg
      721 aggttgaggg cgatgccttg caggccgttg ggaaactcca ccagctccga agcgcgcacg
      781 ccgcgcagcc cgtagacgcg cgcgagattc gcgccgacct cgatgaccgt gccgacttcg
      841 tcttcctgaa gtccggtctt gaagctctcg atctgttgtt tgaggattcc ggcgatttcg
      901 tcagcgttga tcatgcattt ctatcctaag cggttgcaaa aagggtacgc gagagcgcgt
      961 cgaggcgccc ggccacggag ccgtcgatgc ggcgatcgcc catgaggatg cgcacgccgc
     1021 cgatcagcga cggatcgacg acggtagtaa cttcgaactt cttgccgtag aggcgctcca
     1081 gccgttcgac caaagcggaa agctcggcgg acgagagcgc ccgggcggag gtcacggtca
     1141 gcggctccgc accgcgcgcg gcgagctgca gcttgcggta ctcccgaagc agtgcgtcga
     1201 gcagcctttc gcgacgcttg cgcaccagca gcagcagcgt atggagcgcg atctcgctca
     1261 cgcggctgct gaaggccgcc gacagcgcgc gctctttggc atcgcgatcg acgaccggag
     1321 acgtgaagaa ctccttgaag gctggccgct cctcgagcac gcgcgcgatc gcggccaggt
     1381 cgtcgtgtac gcggtccacc tggttccgct cggcggccag cgagaagacc gcttcggcgt
     1441 agcgatgcgc cagtttttcg tcagccatcg gtgccttcca gcgatgcgac gaaacgatcg
     1501 acgagacgcg cgttggcgtc ggcgtcgatc cgctgcgcgg cgtcttcgcg ggcgcgcgcc
     1561 agggctttgt cgagcagttc gacgcgcaac cggtcgcgtg ccgaggcgag cgcgcgcgcg
     1621 aactcggcct gcgcgccgtg cgcggcccgt tcgcccgctt cgcgcgtttc cgcaacggtc
     1681 gcctcgtgct cgcgcttggc ttgcgcttcg gcacgcgtgc ggatcagctc cgcgtcgcgt
     1741 tcggctccgc cgatctcgtt gcgcagcatc tcgagcgtcg ctttggcctc gtcgcggtgg
     1801 cgctcggcct gagcgatctg cttgttatga cgctcctgtg cggccagaat ggccggctgc
     1861 aggtacttga accacagcca cgccagcacg cacatgaaga gaacggccga aacgacctgg
     1921 ctccacaccg cgacctgcac gtagaaagcg tccggcctca ttgcgccgct ccgagggtgc
     1981 gctcgaccat ctgcccggcc agctgacgca ccaggcgatc ttcgccggcg cgcgcgcgct
     2041 ggagttcggc gtcggcgttg cgcgccgctt cttccatcgt cgcagcaacc ttggcggcat
     2101 accgcgccgc gagctcggcc gactcgttcg aggcgtcggc gcgcgctttc gcgacctttt
     2161 gctcggcttc gcggcgcgct tgcgcgcgaa cgcgctcggc ctgttcgcgg agctccttgg
     2221 cctgcgcctg ataatccgcg tagtccgcga cgacgccgtc gatgtactgg cggcgcttgc
     2281 gaatggcttg tccgacggga cgtaagaaca ggacgttcag caacgcgaag aagatcgcga
     2341 agttgacgag ctggatccaa aacgttccgt cgagctggag aaacattccg gcgccgttcc
     2401 cgccccgttc ctagtgtccg gccaaactgg cgaagacgcc gttcggcagg ccggcgacga
     2461 cgatcagcat gtaaaacgcg agaccgagcg cgatgatcgg aaacgcctcc agcacgccga
     2521 cgccgaggaa gaggaacgta aagatgttcg gccgggcctc cggctggcgc gcgattgcct
     2581 cgacggcctt actcgcgacg ataccgtcgc cgatcgccga gccgaacgcg acgccggcga
     2641 cgatgatgcc gaacgccagc agcgtaaaag ctgctaccaa cgcttgagaa gtcaagagcg
     2701 taacctttcc atcaggatgt cagtgatcgt ccgaaatcga caacgccaga tagacgatcg
     2761 ccagcaaggt gaagacgaac gcttggacgg tgccgacaaa gaaattgaaa aattgaatga
     2821 agaacgggag taccgtgacg gccagcgaga gattcaccgg gccgatcgtc accttggctt
     2881 cgacgatcga cgcgatgatc acgaacagca gctcgccgac gaagatattg aagaacaacc
     2941 gcgccgcgag cgtggcggga cgcgcgcact cctccagcac gttgatcgga aataatgccc
     3001 aaaaaggttt gaacaggtgc ttcagccgcg ccgaccccgt catccgcaac ccggcgatcc
     3061 aggtgagaac gaacaccagc agcgccagcg gcacggtcgt gttcaaatcg gcggtcggcg
     3121 aaccgccgaa cggcaggccg aacgccttga acggcagcat gccgaactgg ttcatcagga
     3181 agatgaacac gaagagcgcg atgaagaacg ggacgaacgg ctcgcctttc ggtcccagcg
     3241 tgctggtcgc caagtcggcg atgtagttga cgacgccctc gaccacgact tggcgccggc
     3301 tctgatacgc ggaacggtaa ctggcgccga cccatgcaaa gaacaccagc gacaggacca
     3361 tgacgagcca ggtcgtcacg atcgtatccg catggactcc tccgagcacc ggcaggtgcc
     3421 agaggatgtg ttctccgagt ttttcctgca agccctactc cgagaggtcg gcgtctactt
     3481 tgctgtgaaa gcgcggcgcg cgctataggc gaacattccc aacggaagga agaatccggc
     3541 gaaataccag gccatcgacc accacgggcc aagcgcggcg aacgccaccg ggactatacc
     3601 gaagagggcg attcgcacga aactacttaa gacgaagccg ccgacgaagc gactttcgac
     3661 gagccgctcc ccgcttcgca tcgtccacag gacgttgaga accccgcaaa atccgcccgc
     3721 ggcgagggcg agtgccgggt ggggctgcca gcgcgcgaag accaacgcga cgactgcgac
     3781 gacgagcaag cttcgaacgg acaccgaccg cttgagcgta tggtacgtcg cgagatcggc
     3841 cgccgcttcg gcgtcgctgt agctacgccg ggttgggggg ttatcctgca cgcggtttac
     3901 atcgagcgaa gcagcagccg gacggccgaa tatcctccga ctgccatccc ggcgaacagc
     3961 ccgccggcaa cccacagttc gtggccggtg agccgtccca gccaaatgcc ggccatcagc
     4021 ccggccagca ccgcgatcgc aaacgtcccg ccggctgcga gcaccgggac gacgccgttc
     4081 acggggtcgc ggccgggcgg ccgaccgaag cggccgccac cgcaaagggc gtggggtagg
     4141 gcccgcccct cagccgggca agcagccaag cagcgatccg ctcggcggcg tggccgtctc
     4201 cgtacggatt gaccgcgcgc gccattcttc cgaaatatcg ctcgtcggtc agcagccgcc
     4261 gggccgcctc gaagatgcgc tcgccccgat gtccgaccag ctccagcgtt ccggcgtcga
     4321 gcccttcggg gcgctcggtt tcgtcgcgca tcaccagcac gggctttccg aggcacggtg
     4381 cttcttcttg cacgccgccc gagtcggtga gcacgaaggt cgatccgcgg accgcaaaga
     4441 ccatctgctg ataatcgatc gggtcgacca ggatcacgcc cgggacgtcg ccca
//
LOCUS       DASUCJ010001153         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_898934, whole genome shotgun sequence.
ACCESSION   DASUCJ010001153 DASUCJ010000000
VERSION     DASUCJ010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898934"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFU90_13560"
     CDS             <1..741
                     /locus_tag="VFU90_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5480866.1"
                     /translation="AEMYGKASGCSRGHGGSMHLFGAAQRFYGGNAIVGGGLPLAVGL
                     ALADRILERPRVTCCFFGDGATAEGEFHESMNLAALWRLPVLFVCENNLYAMGTALER
                     YESVTDIRRHAESYGIHAETVDGMNVVDVEVAARRAAEAIRAGEGPRFLENRTYRFRA
                     HSMFDPELYRSKAEVDEWKKRGPIVRFTEWTLGNDLLHSDDLAAIEADVAKLIDDAVA
                     FAEQAPWEPATDLLRDVYAPREAAGGTP"
     gene            738..>1008
                     /locus_tag="VFU90_13565"
     CDS             738..>1008
                     /locus_tag="VFU90_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5480867.1"
                     /translation="MNATRRISYRDAVREALREALLRDPRVFLMGEDVGRYGGAFAVS
                     LGLLAEFGPERIRDTPLSESTFVGAGIGAALGGMLPIVEIMTVNFS"
BASE COUNT          163 a          321 c          365 g          159 t
ORIGIN      
        1 gccgagatgt acggcaaggc gagcggttgc agccgcggcc acggtggctc catgcacctg
       61 ttcggggcgg cgcagcgctt ttacggcggc aacgcgatcg tgggcggcgg cctgccgctg
      121 gcggtgggcc tggcgctggc cgacaggata ctcgaaaggc cgcgtgtcac ctgctgcttt
      181 ttcggggatg gggcgacggc cgaaggcgaa ttccacgaat cgatgaacct ggcggcattg
      241 tggcggctgc cggtcctgtt cgtctgcgag aacaacctct acgcgatggg cacggcgctg
      301 gagcgctacg agtcggtcac cgacatccgg cggcacgccg agtcctacgg catccacgcg
      361 gagacggtcg acggcatgaa cgtggtggac gtcgaagtcg ccgcgcgtcg tgccgccgaa
      421 gcgatccggg cgggcgaggg accgcggttc ctggagaacc gtacctaccg cttccgcgcg
      481 cattcgatgt tcgatccgga gctctaccgc agcaaggccg aagtggacga gtggaaaaag
      541 cgcgggccga tcgtgcgctt caccgaatgg acgctcggca acgatctcct gcattccgat
      601 gacctcgcgg ccatcgaggc cgacgtcgcg aagctgatcg acgacgcggt cgcgttcgcc
      661 gaacaggcgc cgtgggagcc ggccacggat ctgctacggg acgtctatgc gccgcgcgag
      721 gccgcgggag gcacgccatg aacgccacac gccgcatcag ctaccgcgac gccgtgcgcg
      781 aagcactgcg cgaagcgctg ttgcgcgacc cgcgcgtgtt cctgatgggc gaggacgtcg
      841 gccgttacgg tggggctttc gcagtaagcc tgggtctgct cgcggaattc ggaccggagc
      901 gcattcgcga cacgccgctg tccgagtcga cgttcgtcgg cgccggcatc ggtgcggcgc
      961 tcggcggcat gctgccgatc gtcgagatca tgaccgtgaa cttcagcg
//
LOCUS       DASUCJ010001154         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1387976, whole genome shotgun sequence.
ACCESSION   DASUCJ010001154 DASUCJ010000000
VERSION     DASUCJ010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387976"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..849
                     /locus_tag="VFU90_13570"
     CDS             289..849
                     /locus_tag="VFU90_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5480868.1"
                     /translation="MHEMIRVADPALLTHLTGVGSLAGRIAKDLGLDPDEVHHVTIAG
                     RLLDVGKIALPQGALHADEPLDAATMALIEGHAAMGERMVGAIAGLRGYAGWVRSHHE
                     RLNGSGYPDGLHEHDIPFEVRVLSVADVFDAMVCPRPYRAPYSVADAMAHLTANAGSL
                     YDSQVVASLEHLLVAKRRRSGTPTAA"
     gene            complement(914..>1737)
                     /locus_tag="VFU90_13575"
     CDS             complement(914..>1737)
                     /locus_tag="VFU90_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5480869.1"
                     /translation="PVRTPWGCDGPDDNFTYVLKYGTAKPPVFSPSTGIERRGAAPCF
                     QYASAADLLDGAGISWAYYAPDVTNSGGIWSAFDAIWPVRFGPDWAGDVKSPETRIFN
                     DIQSGTLPQVAWVVPAFVNSDHAGSRSTTGPQWVAAIVNAIGESSYWDDTAIVVVWDE
                     WGGWYDHVVPPQIKDPRTHAYEGLGHRVPLIVVSPYAKTGYVSHRRHEIASTLHFIES
                     IFGLPSLGLADARADALGDMFDFSQQPTPFQPIPDARRPSDFKRQPPSLFPPDDD"
BASE COUNT          302 a          532 c          582 g          321 t
ORIGIN      
        1 ggagaacgcg ctgcgttcgc cgagctgctt tcgatcgtac aagacgaagt aagcttccaa
       61 gaccgctggg caatagtcga ttggattcgc cgcgaaatgc acgcggcttc acccgaggag
      121 atcgcagatt tactcgatcg agccgggaat gaagttgcgg ccggcgttcc cgagtgtgac
      181 cggcgcgtcg tactaactcg tttggatgcc ctgcgcgaac gcgcgcacga catcgttgcg
      241 gaacaatccg tcgtccgcgg gactacgccc gaggacttgg tagatgcaat gcatgagatg
      301 attcgggttg ccgatccggc actcctgacg catctcacgg gtgtgggctc ccttgccggg
      361 cgcatcgcca aggacctcgg gcttgatccc gatgaggttc atcacgtcac gattgcgggt
      421 cgtctcctcg acgttggcaa aatcgcgttg ccgcaaggcg cattgcacgc tgacgaaccg
      481 ctcgacgccg ctaccatggc gttgatcgaa ggtcacgctg cgatgggcga acgaatggtc
      541 ggcgcgatcg ccggtttgcg tggttatgcc ggatgggtgc gttcgcatca cgagcgtctc
      601 aacggatccg gctatccgga cggcttgcac gaacatgaca ttccgtttga ggtccgcgtc
      661 ctgtcggtcg cggacgtctt cgatgccatg gtctgcccgc gtccgtaccg cgcaccgtac
      721 tcggtcgccg atgcgatggc gcatctcacg gccaacgccg gctcgttgta cgactcgcaa
      781 gtcgtcgctt cgctcgagca tctgctcgtc gccaagcgcc gtcgttcggg aactccaaca
      841 gcagcgtaag cttctaggaa cgggtctgat tgcttcggat cgccaaacgg cgatgcgagg
      901 caatcagccg cacctagtcg tcgtcgggcg gaaagagcga aggcggctgc cttttgaagt
      961 cgctcggcct tcgcgcgtcc ggaatcggtt gaaacggcgt cggctgctgc gagaagtcga
     1021 acatgtcgcc gagtgcgtcg gcgcgcgcgt cggcgagacc caacgacggc aggccgaaga
     1081 tcgattcgat gaagtggagc gtgctcgcga tctcgtggcg acggtgcgaa acgtaaccgg
     1141 ttttcgcata cggtgagacg acgatcagcg ggacgcggtg tccgaggccc tcgtacgcat
     1201 gcgtacgggg gtctttgatt tgcggcggga cgacgtggtc gtaccagccg ccccattcgt
     1261 cccacacgac gacgatcgcg gtgtcgtccc agtacgagct ctcgccgatc gcgttgacga
     1321 tggcggcgac ccactgcggc ccggtcgtgc tgcgcgagcc ggcgtgatcg gagttgacga
     1381 aggccggcac cacccacgcg acttgcggga gcgttccgga ctggatgtcg ttgaagatgc
     1441 gcgtctcggg gctcttcacg tcgccggccc agtcggggcc gaagcgcacc ggccaaatcg
     1501 cgtcgaacgc cgaccagatg ccgcccgagt tggtgacgtc gggcgcgtag taggcccacg
     1561 agatgccggc gccgtccaga agatccgccg ccgaggcgta ttgaaaacac ggcgccgccc
     1621 cccggcgctc gatgccggtg ctcggggaaa ataccggcgg cttggcggtt ccgtatttca
     1681 ggacgtaggt gaagttgtcg tccgggccgt cgcagcccca gggcgtgcga acgggca
//
LOCUS       DASUCJ010001155         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_150117, whole genome shotgun sequence.
ACCESSION   DASUCJ010001155 DASUCJ010000000
VERSION     DASUCJ010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150117"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFU90_13580"
     CDS             <1..480
                     /locus_tag="VFU90_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480870.1"
                     /translation="ADGTPAPGGANERFEPLTGSLPQRPAVPDRHVFRVGADGTAEPT
                     GTTAPREPSRAIATVRNAPPPAVEPPPPSLTAAADEPRRTKPARRRRSAAIRPGPAGE
                     LESEPFAALPPAEEKPKRTRKAAAATKAPAKRKKEPPAKTPAARKKKATTAKRRSPG"
     gene            complement(458..>1855)
                     /locus_tag="VFU90_13585"
     CDS             complement(458..>1855)
                     /locus_tag="VFU90_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5480871.1"
                     /translation="TYAHSLALENMKRFFALTLGALLGATLVVVPAASTTAAVPAAAV
                     SQADAGEISSAYAHLTTDFYKKVDPQTVLDSVRSQLIFALRTAGVAHTALPAMRSNDA
                     SGANVRAIDREIEVASAESRSKFNVHQMSYVALDGMMRSVKDRYTVFLTPKEFAALNQ
                     DLDGGDFGGTGIVIQIDDQTKYILVENVIPDGPADKAGIQQDDLITAIDGKSTKGLTV
                     QQASEKLRGKEGTRVSLTVQRDGAPVAPIAITRAKIHQLSVYEKMLPGKIGYVALTVF
                     GRDTGSELTAALTRLQQENARALVLDLRDNGGGYLQAAVAVSSKFIPSGPIVSVESRA
                     SNITTLEADDTAIDPLPLVVLVNGYTASASEITSGAIQDSGVGTIMGTKTFGKGVVQT
                     IYPLPDGSAVKITTARYLTPRNRDINHLGITPDVVVSENKHPQYGTPTKDDQLERAVQ
                     YLNDKIAKLNQANGV"
BASE COUNT          304 a          594 c          641 g          316 t
ORIGIN      
        1 gccgacggta cgccggcgcc gggcggagcc aacgaaaggt tcgaaccact gaccggctcg
       61 ttgccgcagc gtcccgccgt tcccgatcgg cacgtctttc gggtcggtgc ggacggcacc
      121 gccgagccga ccggtacgac cgcgccgcgc gaaccgtcgc gggccatcgc gaccgttcgc
      181 aacgcgccgc cgccggccgt cgagccgccg ccgccgagcc taacggccgc cgccgacgaa
      241 ccgcgcagga ccaaacccgc ccgccgccgc cgaagcgcgg cgatacggcc gggaccggcg
      301 ggcgagctgg aaagcgagcc gtttgccgcg ctgccgccgg ccgaagagaa acccaagcga
      361 acgcgcaagg ccgcggccgc cacgaaggcg ccggccaagc gtaaaaaaga accgccggca
      421 aagacgccgg cggctcggaa aaagaaggcg acgacggcta aacgccgttc gcctggttga
      481 gttttgcgat cttgtcgttg aggtactgaa cggctcgttc gagctggtcg tccttcgtcg
      541 gcgtcccgta ctgcgggtgc ttgttctcgc tcaccacgac gtcgggcgta atgccgaggt
      601 ggttgatgtc gcgattgcgc ggcgtcaggt agcgcgccgt cgtgatcttc acggccgagc
      661 cgtcgggcaa cgggtagatc gtctggacga cgcctttgcc gaaggtcttc gttcccatga
      721 tcgtgccgac gccgctgtcc tgaatcgcgc cggaggtgat ttcggaagcc gaagccgtgt
      781 agccgttcac cagcacgacc agcggcagcg gatcgatcgc ggtgtcgtcg gcttcgagcg
      841 tcgtaatgtt cgacgcgcgc gactcgaccg agacgatcgg accgctcgga atgaacttcg
      901 agctcaccgc caccgcggct tgcagataac cgccgccgtt gtcgcgcaga tcgaggacga
      961 gcgcgcgggc gttctcctgc tgcagacggg tgagcgcggc cgtcagctcg gagcccgtgt
     1021 cgcgtccgaa cacggtcaag gcgacgtacc cgatcttacc gggcagcatc ttctcgtaaa
     1081 cgctgagctg atggattttg gcgcgcgtaa tcgcgatcgg cgccaccggc gcgccatcgc
     1141 gctgcaccgt gagcgatacg cgcgtgcctt ccttgccgcg gagtttctcg ctggcctgct
     1201 ggacggtgag ccccttggtg gattttccgt cgatcgcggt gatcagatcg tcttgctgga
     1261 tgccggcttt gtcggccggc ccgtcgggaa tgacgttttc gacgaggatg tacttggtct
     1321 gatcgtcgat ctggatgacg attcccgtcc cgccgaagtc gccgccgtcg aggtcctgat
     1381 tgagtgcggc aaactctttg ggcgtgagga acaccgtgta gcgatccttc accgagcgca
     1441 tcatgccgtc gagcgcgacg tagctcatct ggtggacgtt gaacttcgag cgcgactccg
     1501 cgctcgcgac ctcgatctcg cgatcgatgg cgcgaacgtt ggcgcccgag gcgtcgttcg
     1561 agcgcatcgc cggcagcgcg gtgtgggcga cgccggccgt ccgcaatgca aagatcagct
     1621 gggagcgcac cgaatcgaga acggtctgcg ggtcgacctt cttgtagaag tcggtggtga
     1681 ggtgcgcgta ggcgctggag atctcgccgg cgtcggcctg cgaaaccgct gcggcgggaa
     1741 ctgcggccgt cgtcgaggcg gcggggacga cgacgagcgt tgcgccgagg agcgcgccga
     1801 gcgtcagggc aaaaaagcgt ttcatgtttt ccaatgctag cgagtgagcg tacgt
//
LOCUS       DASUCJ010001156         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_379470, whole genome shotgun sequence.
ACCESSION   DASUCJ010001156 DASUCJ010000000
VERSION     DASUCJ010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379470"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFU90_13590"
     CDS             complement(<1..864)
                     /locus_tag="VFU90_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480872.1"
                     /translation="MQVFPAEKPCRTARCCIADAARSACRIAASRVASKGFGSIPRRA
                     ERRVGFSRDLSAIARVEVNVSTAPLPPGHAWPPEAQGLYAPVYEHDACGLGFVAHIKG
                     TKSHAIIEQGLQILKNLTHRGATGADPLQGDGAGILIQLPDAFFRRACGRLGITLPAI
                     GQYGVGMVFLPQEPASRMACEQEVERAIAAEGQVLLGWRDVPTRNGGLSERTREVEPL
                     IRQVFVARGSRDMDQDALERKLYVIRKRAGHAIQSLALRHGKEFYVPSMSTRTIVYKG
                     MLLADQVGTYYL"
     gene            978..>2255
                     /locus_tag="VFU90_13595"
     CDS             978..>2255
                     /locus_tag="VFU90_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480873.1"
                     /translation="MHAHRPTGWLRPLLTLAALAGVLSLAACGGGGGSPNNPYQPGGA
                     APSVAPATANIYSQIPYVFTVTGGTPPYRITSGNPNDLQVPAEAVGNTFAVLGNVVPA
                     ATTESLTVTDALGRAATATVTINPAVLLPTSITITGNPNCAASGATLCSGQDGTASVK
                     ATGANGIPLAGRAIRFDVVQGDYTISPPGQSPTTSFTVTTDQNGVAAVRLLVAVSAPT
                     QIATIRATDVASGESIVGQFTIAQYTNGSATLTVLPTGTTTFTGPDTATCDAGGMASF
                     YIFGGTPPYTVATNFPQAITIKGAPVLKSGGSFTITTTGVCTSKDGVTLIITDASGRV
                     ATSPTVDNVVGTAAAPPPALVVTPASLSGTCSAGSTFTFLATGGTGTYTNSINPQPAG
                     VTVTPTGANVAVSFSSAVSATTFNITVSSGSQAV"
BASE COUNT          387 a          794 c          720 g          354 t
ORIGIN      
        1 gaggtaatac gtgccgacct ggtcagccag cagcatgccc ttgtagacaa tcgtgcgcgt
       61 cgacatcgac ggcacgtaga actccttgcc gtggcgcagc gcgagcgact ggatcgcgtg
      121 cccggcgcgc ttgcggatca cgtagagctt gcgctcgagc gcgtcctggt ccatgtcgcg
      181 cgagccgcgc gcgacgaaca cctggcggat cagcggctcg acctcgcgcg tgcgctccga
      241 cagcccgccg ttacgcgtcg gcacgtcgcg ccagccgagc agcacctgcc cttcggcggc
      301 gatcgcgcgc tcgacttcct gctcgcaggc catccgcgac gccggctcct gcggcaggaa
      361 caccatcccg acgccgtact gcccgatcgc gggcagcgtg atgccgagcc tgccgcacgc
      421 gcgccggaag aatgcgtcgg gaagctggat caggatcccc gcgccatcgc cctgcagcgg
      481 atcggcgccg gtcgcgccgc ggtgcgtcag gttcttcagg atctgcagcc cctgctcgat
      541 gatcgcgtgg gacttcgttc ccttgatgtg cgcgacgaag ccgagcccgc aggcatcgtg
      601 ctcgtagacc ggagcgtaga ggccctgggc ctccgggggc caggcgtgac cgggcggcag
      661 tggggcagta ctcacgttga cctcgaccct cgcaatcgca gacagatccc ggctgaatcc
      721 gacgcggcgc tcggcacgcc tcgggatgga gccgaaacct ttcgatgcta cgcgcgatgc
      781 tgcgatgcgg caagcagatc gcgcggcgtc cgcgatgcaa caacgcgcgg tccggcaggg
      841 cttttccgcc ggaaagactt gcaccggcca cgatgttagg ctttaatctg aacaaagtcc
      901 gctagatcat tgttgctacg ttcaattcag ccgctgccct ttcgcatctt taggacaaca
      961 tcatcaggaa acccgccatg cacgcacacc gaccgactgg ctggctgcgc ccgctcctca
     1021 cccttgccgc gctggctggc gtgctctccc ttgcggcgtg tggtggcggc ggcggctcgc
     1081 cgaacaatcc gtaccaaccg ggcggcgccg cgccgagcgt ggcacccgca acggctaaca
     1141 tctattcgca gattccgtac gtgttcacgg tcaccggcgg gacgccgccc taccggatca
     1201 cgtctggaaa tccgaacgac ctgcaggttc cggccgaagc ggtaggaaac accttcgctg
     1261 tgcttggcaa tgtcgttccc gccgcgacga ccgaatcgct cactgtcacc gacgcgctcg
     1321 ggcgcgccgc gaccgcgacc gtgacgatca acccggcggt gctgctcccg acgtcgatca
     1381 cgatcaccgg caatccgaac tgcgcggcgt cgggcgcgac gctttgctcg ggacaggacg
     1441 ggaccgcctc ggtgaaggca accggtgcga acggcattcc gctcgccggg cgcgcgatcc
     1501 gtttcgacgt cgtccagggc gactacacga tttcgccgcc cggccagtcg ccgacgacgt
     1561 cgttcacggt gacaaccgat cagaacggcg tcgcggcagt gcgcctgctc gtagccgtca
     1621 gcgcaccgac gcagatcgcg acgatccgcg ccaccgacgt cgcgagcggc gaaagcatcg
     1681 ttgggcagtt tacgatcgcg cagtacacga atggcagcgc gaccctcacc gttctgccga
     1741 ccggcacgac gacgttcacc gggcccgata ccgcaacctg cgacgcgggc ggcatggcct
     1801 cgttctacat cttcggcggc acgccgccgt acacggtcgc gacgaacttc ccgcaggcga
     1861 ttacgatcaa gggtgcgccg gtgctgaaga gcggcgggag cttcacgatc accacgaccg
     1921 gcgtctgcac gtcgaaggac ggagtaacct tgatcatcac cgatgcgagc ggtcgcgtgg
     1981 cgacgagccc gaccgtcgac aatgtggtcg gcaccgcggc ggccccgcca ccggcgctcg
     2041 tcgtcacgcc ggcttcgctg tcgggcacct gcagcgccgg atcgacattc acgttcctcg
     2101 ccacgggagg aaccggcacc tacaccaatt cgatcaaccc gcagccggca ggcgtaacgg
     2161 taacgccaac gggcgcgaac gtggccgtgt cgttcagcag cgcggtaagc gcaacgacat
     2221 tcaatatcac cgtctcgtcg ggcagccagg cggtt
//
LOCUS       DASUCJ010001157         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_287281, whole genome shotgun sequence.
ACCESSION   DASUCJ010001157 DASUCJ010000000
VERSION     DASUCJ010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287281"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFU90_13600"
     CDS             complement(<1..952)
                     /locus_tag="VFU90_13600"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5480874.1"
                     /translation="MGVTLRGMAEGSFARGLFFGAIEEDVIFPWRECPPGETHTLQVL
                     IDDTRRFFEEHVDSAAIDRAEQIPERVFAGLSQRRVFGLCAPRALGGLGFSATAGARA
                     VQEIAGLVLGIGITVAAHHSLALRALLLFGTDAQKARYVPRLAHGESIAAFALAEPGA
                     GSDAAGIQTIARTVTHTSSAASTARAASGYSLDGAKGWTTNGGFADLFTVFARTSAPD
                     EGAKPKITAFLVERGRGVTSGPNQSKLGVRGASTTLVRFDGVHVPAENVLGEIGRGFR
                     VAMQSLDHGRLDLSSASVGVCQRAIKLTLDRCRQRRAFGRP"
BASE COUNT          205 a          451 c          410 g          207 t
ORIGIN      
        1 tcgggcggcc gaaagcccgc cgctgccggc agcggtccaa ggtgagcttg atggcgcgct
       61 ggcagacgcc gacgctcgcg ctcgaaaggt ccagcctccc atgatcgagc gactgcatcg
      121 ccacccgaaa gccgcggccg atctctccca gcacgttttc ggccggcacg tgcacgccgt
      181 cgaagcggac gagcgtcgtc gacgccccgc gcaccccgag cttcgattgg ttggggcccg
      241 acgtgacgcc gcgcccgcgt tctacgagga acgccgtgat cttcggcttg gcgccttcgt
      301 ctggcgccga ggtccgggcg aagaccgtga acaagtccgc gaatccgccg ttggtcgtcc
      361 atccctttgc gccatcgagg gagtagccgc tcgccgctcg ggccgtactc gcggcgctcg
      421 aagtgtgggt gaccgtgcgc gcaatcgtct gaatgcccgc ggcgtcgctt cctgcgccgg
      481 gctcggccag agcaaacgca gcgatgctct cgccgtgcgc gagtcgaggg acgtagcggg
      541 ccttctgcgc gtcagtgcca aagagcagga gggcccgtaa cgccagcgag tggtgcgccg
      601 ccaccgtgat cccgatgccg agaacgagcc cggcgatctc ctgcacggcg cgcgcccccg
      661 ccgtcgcgga aaagcccagc cccccgagcg ccctgggggc acagaggccg aacactcttc
      721 gctgcgaaag gcctgcgaaa acgcgctccg gtatctgctc ggcccggtcg atcgccgccg
      781 agtcgacgtg ctcttcgaag aaccggcgcg tgtcgtcgat aagcacctgc agcgtgtgcg
      841 tctcgccggg cgggcactct ctccacggaa aaataacgtc ttcctcgatc gcgccgaaga
      901 aaaggccccg tgcgaacgat ccttccgcca tgccgcgtaa cgttactccc atctcgctct
      961 cgcccctgtc gtcgcgtcat cgctccgggc cgtcgcctcg cagttgagcc gcaacatcgc
     1021 cgcgccgtgc gtgctggccg gaccatcgca cgttttcggc tcacaaatcg cgattgcgca
     1081 ttgccccttc cggcatcggt tgctagccaa gaaatacgga cggcgcggcc tccgtgagga
     1141 ggaacgggca ggcacgtggc ttctcgaatc gaagcgtttc aaacggtgct cgaccgcgca
     1201 cgcagcacgc gcggcgttgc cttgatgggc atatgcaacg tgacgcccga ttcgttcagt
     1261 gacggcggag cct
//
LOCUS       DASUCJ010001158         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1219901, whole genome shotgun sequence.
ACCESSION   DASUCJ010001158 DASUCJ010000000
VERSION     DASUCJ010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219901"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFU90_13605"
     CDS             <1..377
                     /locus_tag="VFU90_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5480875.1"
                     /translation="WAGTRDGVPLAVHVRMIPNGFELAYRGIEVKTFVYTEREAHYAR
                     LMPAKTLSGSEKAVRSPMPGLVVLIAVAEGEEVKAGESLAVVEAMKMENVLRAERDGI
                     VKKIRVKPGDSVAVDAVIMEFA"
     gene            538..>1034
                     /locus_tag="VFU90_13610"
     CDS             538..>1034
                     /locus_tag="VFU90_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480876.1"
                     /translation="MHALRFLFSPSGRLRPQAFIAAIAVVYAAGVASHALTTADVIAR
                     VGLWAFVAVQALLVWIWFALHAKRLRDAGRGTGLAAGVAILYVLSIVLLVIVAAAFLN
                     TAAADAADPSSATALGLILLVAVVAALAGSAHYDIGWLLVAVLTLAALIPVIVALLFT
                     LWTATR"
BASE COUNT          150 a          338 c          352 g          194 t
ORIGIN      
        1 tctgggccgg cacgcgcgac ggtgtcccgc tggccgtcca tgtgcgcatg attccgaacg
       61 gcttcgagct cgcctaccgc ggcattgagg tgaaaacctt cgtctatacc gagcgcgaag
      121 cgcactacgc gcggctgatg ccggcgaaga cgctgtccgg gagcgagaaa gccgtgcgct
      181 cgccgatgcc cgggctcgtc gtcttgatcg ccgttgccga aggcgaggag gtaaaggccg
      241 gcgagagctt ggctgtggtc gaggcgatga agatggagaa cgtcctgcgc gccgagcgcg
      301 acggcatcgt caagaagatc cgggtcaagc ccggcgacag cgtcgccgtc gacgccgtaa
      361 tcatggagtt cgcgtagcgt cgatcattcc gggtcgagcc aaaggcgcga accccgaatt
      421 catagccgcg agatttgcaa acgcttcccc gtcccggttc tgcaatactg gggctatgga
      481 ttccgggcgc gccgcagagc ggcgccccgg agcgacgtgg aatgacggtg actgaacatg
      541 cacgcgttgc gcttcctgtt ctcgccgtcc gggcggctgc ggccgcaggc gttcatcgcc
      601 gccatcgccg tcgtctacgc cgccggtgta gcctcgcacg cgctcacgac ggccgacgtt
      661 atcgcccgtg tcggattgtg ggcgttcgtc gcggttcagg cgctgctcgt ctggatctgg
      721 tttgcgctgc acgccaagcg cctgcgtgac gcgggccgcg gcacggggct cgcggctggc
      781 gtcgccatac tctacgtgct gtcgatcgtg ctgctggtga tcgtcgccgc ggcgtttcta
      841 aataccgcgg ccgcggacgc tgccgatcca agcagcgcca ccgcgcttgg cctcatcctg
      901 ctggtggcgg tcgtcgccgc gctggcgggc tccgcgcact acgatatcgg ttggctcctg
      961 gtcgcggttt tgacgcttgc cgccctcata ccggtaatcg tcgcgctcct gttcacactg
     1021 tggacggcaa cgcg
//
LOCUS       DASUCJ010001159         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_837586, whole genome shotgun sequence.
ACCESSION   DASUCJ010001159 DASUCJ010000000
VERSION     DASUCJ010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837586"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFU90_13615"
     CDS             complement(<1..572)
                     /locus_tag="VFU90_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480877.1"
                     /translation="MIASRRVAVLPGRPYPLGASWNGAGVNFALFSENAEKVELCLFD
                     PRGRREIARIALPEYTDSVWHGYLPDARPGLLYGYRVYGPYDPAAGHRFNHHKLLLDP
                     YAAMVAGGIVWSDAHFGYRVGSPRADLSFDRRDNAAGMPKCVVVDPAFTWGNDRLLHT
                     RWHDLIIYELHVRGYTMKHPAVPPELRGTFA"
     gene            complement(569..>1342)
                     /locus_tag="VFU90_13620"
     CDS             complement(569..>1342)
                     /locus_tag="VFU90_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5480878.1"
                     /translation="LGFGYKWNMGWMHDTLRYFGYDPVYRRYHQSDLTFGLVYAFSEK
                     FVLPLSHDEVVHGKRSLLEKMPGDRWQKFANLRALLALQYAHPGKKLLFMGDEFGALR
                     EWDHDAQLDWGLTADPMHAGVARLVADCNRLYRNTPALFELDAEPAGFEWIDFADASN
                     SVVAFARRARSAPCAVAVLNATPVVRRDYRLGVPQPGTYREAINTDSQFYGGSNVGNG
                     GALESAPVACHGRADSLLLTLPPLATLIFTLDAPPGACA"
BASE COUNT          236 a          425 c          477 g          204 t
ORIGIN      
        1 gcgaaggtgc cgcgcagctc cggcggtacg gcggggtgct tcatcgtgta gccgcgcacg
       61 tgcagctcgt agatgatcaa gtcgtgccag cgggtatgca gcagccggtc gtttccccac
      121 gtaaacgcgg gatcgacgac gacgcacttc ggcatgccgg cggcgttgtc gcgccggtcg
      181 aacgagagat cggcgcgcgg cgagccgacg cgatagccga agtgcgcgtc gctccacacg
      241 atcccgccgg ccaccatcgc ggcgtacggg tcgagcagca gcttgtgatg gttgaagcgg
      301 tgaccggcgg cgggatcgta cgggccgtac acgcggtagc cgtagagcag cccgggacgg
      361 gcgtcgggca aataaccgtg ccacaccgaa tcggtgtact cggggagcgc gatgcgggcg
      421 atttcgcgcc ggccgcgcgg atcgaacaag caaagctcga ccttctcggc gttttccgag
      481 aacagcgcga agttcacgcc ggcgccgttc cagctggcgc ccaacggata cggccggccg
      541 ggaaggacgg cgacgcggcg cgaggcgatc atgcgcaggc gcccggcgga gcgtcgaggg
      601 tgaagatcag cgtcgcgagc ggcgggaggg tgagcagcag cgagtcggcg cgcccgtgac
      661 acgcgaccgg cgcgctttcg agcgcgccgc cgttgccgac gttgcttccg ccgtaaaact
      721 gcgagtcggt gttgatcgct tcgcgataag tgccgggctg cggaacgccg agccggtaat
      781 cgcggcgtac gaccggcgtc gcgttgagca ccgctacggc gcatggcgcc gaccgggcgc
      841 gccgcgcgaa ggcgacgacg ctattggaag cgtcggcgaa gtcgatccac tcgaatccgg
      901 ccggctcggc atcgagctcg aagagcgccg gagtgttccg gtacaggcga ttgcagtcgg
      961 caaccagccg cgcgacgccg gcgtgcatcg gatcggccgt caatccccag tcgagctggg
     1021 cgtcgtgatc ccactcgcgc agcgcgccga attcgtcgcc catgaacagc agcttcttgc
     1081 cgggatgtgc gtactggagc gcgagcaagg ctcgcaggtt ggcaaacttc tgccagcggt
     1141 cgcccggcat tttttccaag agcgaacgct tgccgtgcac gacctcgtcg tgcgagagcg
     1201 gcagcacgaa cttttcgctg aacgcgtaca ccagcccgaa ggtgagatcg ctttgatggt
     1261 agcggcgata gaccggatcg tacccgaagt atcgcagcgt gtcgtgcatc cagcccatgt
     1321 tccacttgta gccgaacccc ag
//
LOCUS       DASUCJ010001160         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_745951, whole genome shotgun sequence.
ACCESSION   DASUCJ010001160 DASUCJ010000000
VERSION     DASUCJ010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745951"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFU90_13625"
     CDS             <1..635
                     /locus_tag="VFU90_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480879.1"
                     /translation="PEGGTDADLGRRHAEVFLDLLRGEGFARPNPRPMFPNPPGLLRL
                     EPHSPGLRDRIWWGSASNATAIWAAQHGMNLQSSTLKEDESGEPFHVQQARQIRIFRQ
                     AWTDAGHTREPRVSVSRSIFALMNDLDRAYFGRDESSDQIGLIDNMRAVFGRSYAAEP
                     DVLVEALRGDEAIAEADTLLLTVPNTLGVDYNAHVIESILTHVAPALGWR"
     gene            complement(658..1317)
                     /locus_tag="VFU90_13630"
     CDS             complement(658..1317)
                     /locus_tag="VFU90_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5480880.1"
                     /translation="MPGLSRFVGIAAAAWFACAIVSCSSSTSPNGPVGSVQVSPSVDT
                     VAVGETTALRATVMSHGGQTLSGQHVFWNTGKASVATVSDAGIVTGRAQGTAQVAASA
                     GGASGIATITVLPPRVASVSVSPGRDTIVLPGTAQLTATLFDEHHEPLGNRTVTWASN
                     LPNVANVNRNGLVTGVAAGTAIITASSGSASGSAAIVTVPAPKPPPNPKPPPGDGHHK
                     P"
     gene            1562..2479
                     /locus_tag="VFU90_13635"
     CDS             1562..2479
                     /locus_tag="VFU90_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5480881.1"
                     /translation="MTVLVVGSIALDSVETPFGRAENVLGGSAVFFAASASLLTDVQM
                     VGVVGSDYPVDQLDVLAERGVDLTGIERAPGESFRWRGRYRHDLNTAETLETRLGVFS
                     HFRPKIPEAFRAAPFVFLGNIDPRLQLDVLDQVDKPTLVACDTMNFWIESRRSDLLEL
                     LGHVDLVTLNDAEARQLTEEANLVKAARWIQSHGPKTVIIKKGEHGALMFGPRSVFFS
                     PAFPLENVFDPTGAGDSFAGGFIGYLARCGTVTDTNLRRAVVYGSAMGSFAVERFSIT
                     RFLEIGEDDIAARVQEFHRLVSVEQEIPA"
     gene            2476..>2804
                     /locus_tag="VFU90_13640"
     CDS             2476..>2804
                     /locus_tag="VFU90_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5480882.1"
                     /translation="MSVPLDYRDAGVDIDAADAAKQRIKALVESTFTAGARGKFGGFG
                     GQFRVPPGMRAPVLVSSADGVGTKIKVAIEANRHDTIGHDLVNHCVNDILVQGALPLF
                     FLDYVAFG"
BASE COUNT          446 a          943 c          939 g          476 t
ORIGIN      
        1 cgcccgaagg gggaaccgac gcggatttgg gtcggcgtca tgccgaagtc tttctcgatt
       61 tattgcgcgg cgaaggattc gcccgaccca atcctcgacc catgtttccc aatccgcccg
      121 ggctgcttcg cctcgagccc cattccccgg gactccgcga ccgcatctgg tggggatctg
      181 cgtctaacgc aaccgcgatc tgggccgcgc agcacgggat gaacttgcaa agctctaccc
      241 tcaaggaaga cgagtcgggc gagccgtttc acgttcagca ggcccgccag atccgcatct
      301 tccggcaagc ctggaccgac gccgggcata cgcgcgagcc gcgcgtgtcg gtgtctcggt
      361 ccatctttgc gctcatgaac gatctcgacc gcgcctactt cggtcgcgac gagagcagcg
      421 atcagatcgg gctcatcgac aacatgcggg cggtgttcgg ccggtcgtat gcggccgagc
      481 ctgatgtgtt ggtcgaagca ttgcggggcg atgaagccat cgccgaagcc gacaccctcc
      541 tcctgaccgt gcctaacacg ctgggtgtcg actacaatgc gcacgtgatc gagtcgattc
      601 tgacgcacgt cgcgcccgcg ctggggtggc gatagatggt gatggccgtc ccacgggtca
      661 cggtttgtgg tggccgtccc ccggcggagg cttcgggtta ggcggcggct tgggcgccgg
      721 aacagtgacg atcgccgcgc tgcctgacgc gctcccgcta ctggcggtga tgatcgcggt
      781 gccggccgct acgccggtga cgagaccgtt gcgattcacg ttggcaacgt taggcagatt
      841 gctggcccac gtcaccgttc ggttgccgag cggctcgtgg tgctcgtcga acagcgtcgc
      901 cgtgagttgc gcggtacccg gcagcacgat ggtgtcgcgg cccggagaca cggacaccga
      961 agccacgcgc ggcgggagca ccgtgatggt cgcgatgccg gacgcgccgc cggcgctcgc
     1021 ggcgacctgc gcggtccctt gcgcgcggcc ggtcacgatg ccggcgtcgg acaccgtggc
     1081 cactgacgct ttgccggtgt tccagaacac gtgttgaccg gagagggtct ggccgccgtg
     1141 gctcatcacc gttgcgcgca gggcggtggt ctcgccgacg gcgacggtgt cgacggaggg
     1201 cgagacctgc accgatccca ccggtccgtt aggcgacgtg ctcgagctgc acgacacgat
     1261 cgcgcacgcg aaccacgcgg cagcggcgat tccgacgaaa cgggacagac ctggcatcag
     1321 gcgcctgacg ggtcgtgccg gctgggcgtt ggcgcaccgc cggcggttca cgcgccagcg
     1381 ttgcaaggca tgcgccgcga ggcgagggaa gcgtgcgctg acgcgggcgc cgagcatcag
     1441 accaatgccg acccgctttt gtggacaccg caatgacccc cagagtagct tcagctctcg
     1501 gtgcccgagc gaatgtagcg gcgtcgcgct acctttcggt ctcaaccgct ctcgaacaca
     1561 catgaccgtc ctcgtcgtcg gctccattgc gctcgactcg gtagaaacgc ccttcggccg
     1621 cgccgagaac gtgctcggcg gctcggcggt cttcttcgcg gcatcggcaa gcttgctcac
     1681 cgacgtccag atggtcggcg tcgtgggcag cgactacccg gtcgaccaac tggacgtttt
     1741 ggccgagcgc ggcgtggacc tcacggggat cgagcgcgcc ccgggcgaat cgttccgctg
     1801 gcgcggccga taccggcacg acctcaatac ggccgaaacc ctcgagaccc ggctcggcgt
     1861 cttctcgcat tttcgtccca agattcccga agcgttccgc gccgcgccgt tcgtcttcct
     1921 cggcaacatc gatccgcggc tccagctcga cgtgctcgat caggtggaca agcccacgct
     1981 ggtcgcgtgc gacaccatga acttctggat cgaaagccgc cgcagcgacc tcctcgagct
     2041 gctcgggcac gtggacctcg tcacgctcaa cgacgccgaa gcgcgccagc tcaccgagga
     2101 agcgaacctc gtgaaggcgg cgcgctggat ccagtcgcac ggccccaaga ccgtgatcat
     2161 caagaagggc gagcacggcg cgctcatgtt cggacccagg agcgtgttct tctcgccggc
     2221 ctttccgtta gaaaacgtgt tcgaccccac gggcgcgggc gattcgttcg ccggcgggtt
     2281 catcggctat ctcgcgcgct gcggcaccgt caccgacacg aatctccgcc gggccgtggt
     2341 gtacggctcg gccatggggt cgttcgccgt cgagcgcttc tcgatcacgc ggttcctcga
     2401 gatcggcgaa gacgacatcg cagcgcgcgt gcaggagttc catcggttag tcagcgtcga
     2461 gcaggagatt ccggcgtgag cgtgccgctc gactaccgcg acgccggggt cgacatcgac
     2521 gcggccgacg cggccaagca gcgcatcaag gcgctggtcg agtcgacgtt cacggccggc
     2581 gcgcgcggga agttcggcgg attcggcgga cagttccgcg ttccgccggg catgcgcgct
     2641 ccggtgctgg tgtcgagcgc cgacggcgtc gggaccaaga tcaaggtggc gatcgaggcc
     2701 aaccgccacg acactatcgg ccacgacctc gtcaaccatt gcgtgaacga catcctggtg
     2761 cagggcgcgc tgccactgtt ctttctcgac tacgtggcgt tcgg
//
LOCUS       DASUCJ010001161         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_990474, whole genome shotgun sequence.
ACCESSION   DASUCJ010001161 DASUCJ010000000
VERSION     DASUCJ010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990474"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1058)
                     /locus_tag="VFU90_13645"
     CDS             complement(96..1058)
                     /locus_tag="VFU90_13645"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5480883.1"
                     /translation="MTAQEERDLGFGSVVARESRKRLLNRDGTFNVERRGLRYFQSLS
                     LYHTALSISWPRFLAAVVSAYLVINVVFACAFLLCGHGALYTPPTGMPEGRFLQAFFF
                     SVETFGTIGYGNVYPVGVLPGLVMVVEALTGLLVLAMSTGLIFARFSRPTARIRFSTN
                     AVVAPYRGITALMLRIVNERRNQIVEVEAQLLFSRRSADDGSARVFDELGLERRRVVF
                     FPLAWTIVHPIDETSPLFGLTPADLREMDAEFLVLLKGIDETFSQTVHARSSYRDDEI
                     TWNAQFTSMFNPPTPDGVVSIDMDRLDAIERMRTGPAPLNAAAD"
     gene            1323..2462
                     /locus_tag="VFU90_13650"
     CDS             1323..2462
                     /locus_tag="VFU90_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5480884.1"
                     /translation="MPLTDSIRTVAWWRQLEGRRVIARRAGMAGAVVGVATVLALLFA
                     LGRYLAPPHRTPSSLLPILVWELARWYVWLLLLPVLRRALDKSVATQFAAVFGISAVH
                     TLVIVAVEQVVPEFLAGAASGAIASFAGHAGIMAASDLLACAALVAALHAIRYRDRFR
                     ERDQRATVLQAQLDRAELELLGLRIRPHFLFNTLNAVSGLMHDDVDAADEMLSSLGDL
                     LRSALAAGGAQEIPLREEVDFLRRYLAIMRLRLGDRLTTNIELDAGVADTLVPNLVLQ
                     PLVENAVRYGASRLTGTVHVEVRAERCPSGVRLRVIDNGPGLPALREGGRRRDGIGLR
                     TTRERLRQTYGTAYRLELINRPPPNGGVEAVLVLPFRPDVRRRVS"
BASE COUNT          412 a          877 c          848 g          436 t
ORIGIN      
        1 gggcgcgtgg accgccgtgt tcgagcggcg cgggtcgcgc tgggtgatcg tgcaggagca
       61 cctgtccgac gcgccaccca tcgggtgacg ttccgttagt ccgcggccgc gttcaggggc
      121 gcaggccccg tgcgcatgcg ctcgatcgca tcgagccggt ccatgtcgat gctcaccaca
      181 ccgtccggcg tgggcgggtt gaacatcgac gtgaactgcg cgttccaggt gatctcgtcg
      241 tcgcgatacg aagaccgcgc gtgcaccgtc tgcgaaaacg tctcgtcgat ccctttgagc
      301 aacacgagaa actcggcgtc catctcgcgg agatcggccg gcgttaggcc aaagagcgga
      361 ctcgtctcgt cgatcgggtg cacgatggtc caggcaagcg ggaagaacac gacgcggcgc
      421 cgctccaagc cgagctcgtc gaacacgcgc gccgacccat cgtcggcgga gcgccgcgag
      481 aacagcagct gtgcctcgac ttccacgatc tggttgcggc gctcgttgac gatgcgaagc
      541 atgagcgccg tgatgccgcg gtacggcgcc accacggcgt tggtgctgaa ccggatccgc
      601 gccgttggac gcgagaagcg cgcgaagatg agccccgtcg acatcgccag caccaggagc
      661 ccagtcaacg cttccaccac catcaccagc cccggcagca cgcccacagg atagacgttg
      721 ccgtagccga tggtgccgaa cgtctccacc gaaaagaaaa acgcctggag gaacctgccc
      781 tccggcattc cggtgggcgg cgtgtagagc gcgccgtgtc cgcacaacag gaaagcgcac
      841 gcgaagacga cgttgatcac gaggtacgcg ctcacgaccg cagcgagaaa ccgcggccac
      901 gagatcgaga gcgccgtgtg atacaggctc agcgattgaa aatatcgaag cccgcgacgc
      961 tcgacattga acgtcccgtc gcgattgagc agtcgttttc ggctctcgcg tgcgacgacg
     1021 ctgccgaaac cgaggtcgcg ttcttcctga gccgtcatgc gccgacatca ctccggttga
     1081 aagcccaacg acaacgagcg gtcgcgctcg tttccggacc gatcaatgat accgcgtcca
     1141 aacgcggcga caacggtccg ctgtacgcca ttgaccgaag cacgaggccg cgccgcgcgt
     1201 caggtcgctg caccgatccg gcggcctcga ccgaaaaatc tttcgatgcg ccggtgtccg
     1261 ggctcgttac gtgtagcaat tttgctgggg ggggaacgct tcgtcatcgt ggttccatgt
     1321 ctttgccgct cacggactcg attcgcacgg ttgcctggtg gcgtcagctg gagggacgca
     1381 gggtaatcgc gcggcgcgcc gggatggccg gggcagtcgt cggcgtcgcg accgttctcg
     1441 ctctgctgtt cgcgctcggg cgctacttgg ctccgccgca ccgcacaccg tcgtcgcttc
     1501 ttcccatcct cgtgtgggag ctggcccgct ggtacgtgtg gctccttttg cttccggtgc
     1561 tgcgtcgagc gctcgacaaa tcggttgcga cccagttcgc ggcggtgttt gggatctcgg
     1621 cggtccatac cttggtgatc gtcgcggtcg agcaagtggt tcccgaattt ctcgccggcg
     1681 ccgcctcggg cgcgatcgca tcgttcgccg gacacgccgg catcatggcc gcatcggacc
     1741 tgctcgcctg cgccgcgctc gtcgcggcgc tccatgcgat ccggtatcgc gaccgcttcc
     1801 gcgagcgcga ccagcgcgcg acggtgctgc aggcgcagct cgatcgcgcc gagctcgagc
     1861 tgctcggcct cagaatccgt ccgcactttc tcttcaacac gctcaacgcc gtgtcgggat
     1921 tgatgcacga cgatgtcgat gcggccgacg agatgctgtc ttcgttaggc gacctgcttc
     1981 gctcggcact cgcggccggc ggcgcgcagg agattccact gcgcgaggaa gtggacttcc
     2041 tccgccgcta tctggcgatc atgcggcttc gcctgggcga ccggctcacg acgaacatcg
     2101 agctcgacgc cggcgtggcg gacacgctcg tcccgaatct cgtgctgcag ccgttggtcg
     2161 aaaacgcggt gcgctacggc gcgtcgcgtc tcacgggcac ggtgcacgtc gaggttcgcg
     2221 ccgagcgatg cccgagcggc gtccggctcc gcgtgatcga caacggccct ggcctgccgg
     2281 cgctgcgcga gggcggccgc cgccgcgacg gcatcgggct ccgcacgacg cgcgagcgct
     2341 tgcgccagac gtacggcacc gcgtaccggc tcgaactgat caaccggcct ccgcctaacg
     2401 gaggtgtgga ggccgtcctc gtgctgccgt tccgtcccga cgtgcgccgc cgcgtctcct
     2461 gagccgtggg tcccggacgc cgcatccgaa tcatgatcgt cgacgacgag ccgccggcgc
     2521 gcgcccgatt gcgccggctg ctcgagagcg aagacggcgt cgaattgctc acc
//
LOCUS       DASUCJ010001162         5967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_669450, whole genome shotgun sequence.
ACCESSION   DASUCJ010001162 DASUCJ010000000
VERSION     DASUCJ010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669450"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFU90_13655"
     CDS             <1..782
                     /locus_tag="VFU90_13655"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5480885.1"
                     /translation="PSDDPVTYRYNVPQNAFAVIAMRLLARFAREAFDDPKLAADATT
                     LGNQVQVGIERYGKTWDPTRGWTYVYETDGYGNDNLMDDANVPNLTALPYLGWCATDD
                     PVYLDTRRFTLSFSDPYYYTGKYATGLGSPHTPKGWVWPLGIIGAALSTRRRQEIAQA
                     IDMLDQSDTLNGLMHESVDPNDPSRFTRPEFGWANAFWADLLFRTVAGYRGIPYVTVN
                     TVVPLEQVSEIPTITPLSVQLADTAELNSTLGYLLSTQRPP"
     gene            784..2055
                     /locus_tag="VFU90_13660"
     CDS             784..2055
                     /locus_tag="VFU90_13660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5480886.1"
                     /translation="MHLLLAALLTLPPGVRSAVDATVARVMAERHVAGLSLGIARAGT
                     TLYVRGYGLRDVGGRLAARGDTPYPVGSIAKQFVAALILQDAAAGTIALDAPLSRYLP
                     AASTDAATVAQVLGQTGGIAADAGDLRAPGQTLVEAIAGAPPAAAPGTAWTYSNANYV
                     LLEAALERVDGVGFADLLRRRIVEPLGLGSTGYGIPAGGVAALGYAWDGGWHAVAPSG
                     VADDRTRLAGGMYSSVPDLLRWLDALRSGRVVTPDRFVAMTSSGKLSGGIPTNYGFGF
                     FIADWFGYRVAEHPGYVDGFSAQDALVLDDGLEVAVLANGMAVDLTPLTMSLVAAIDR
                     PRDANLAALPSQPPQNENLAITADVKALVETPGFAALGALQSIEFVERSLAGGTTYDK
                     YRLTFSTGQWWVTVGYRENGPIEALTLSPVQ"
     gene            2052..3470
                     /locus_tag="VFU90_13665"
     CDS             2052..3470
                     /locus_tag="VFU90_13665"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5480887.1"
                     /translation="MTHYLRLGAIWVLAAMAAWSVPAPCPAQVLIVPLTGKRVHDVQA
                     AQLFHTLFNDFFLEDDATTYVQTGDIPAMWLRDSSAQTIPYVRFQTAFPILRGRFAGV
                     IERNARNIVTDPYANAFQADYHVWERKWEVDSLAWPMVLTWVYWRVTRDRTIFTPELH
                     VAMQKIVSTYLCEERHSRCNAYQYPYRVRTNYAYAAGTGLIWGAFRPSDDPVQFRFNI
                     PQNAIAVVALHEIEGLALDGYADRDLAQSARALAARVQVGVERYGRFFDAGRRQWMYA
                     YETDGFGRYNLMDDANLPNITTLPYIDWCSNLDPTYRNSRAFVQSKNNPYFYSGRYAA
                     GLGSPHTPPRYVWPLGIIGGALTARSSGDVAEAITTLAITDGESGTIHESFYDDGYWR
                     YTRGEFGWANALGAELLFRSLAGYPSTQFDPGGPVLPFQSRTQTPTLVLPFTQAENAG
                     KIVAALAGLLHVEGTMPHLRSR"
     gene            3504..>5967
                     /locus_tag="VFU90_13670"
     CDS             3504..>5967
                     /locus_tag="VFU90_13670"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5480888.1"
                     /translation="MNQGYYRYPTIAGDRIVFVCEDDLWSVPAAGGAATRLTASFGAC
                     SFPRLAPDGESIAFVSTDEGSPEVYVMPAAGGVPERLTFLGAVLANVVGWSPDGSDIY
                     FVANPTSWDDQPRPFAIARDGGTPRPLELGHARAFARFGENGTVIGRNAADPARWKRY
                     RGGTAGEVWIDAGGTGTFARIPLPNGNPTWPMPIGDRVYFLADHEGIGNIYSCEFDGA
                     DLRRHTHEAEYYARFPSTDGTRIVYSAGGEILCLDLAEDRVTRVPVATNSPAPQTVRR
                     FEDASESLEHFAPHPDGTRLAFVSRGQAFTMPFFDGAAVAYGTGGTGRVRHAEWLCDG
                     KRFACVTDVNGYEQIAVQRAEPANEAPELVTTGDIGRVTDLTCSPVADVVAFANHRHE
                     LCLLDLDDGSVRVVDRSPAHRIGDLAFSPDGRFIAYVWWPTQGTSIVRVAKIKSGKVH
                     DVSSPLRIDQSPAWDPDGKYLYFISTRDFNPVYDALQFELSFPQASRPFVVTLRDDVA
                     SPFVPPPKPVHRDHEHDRDDRKKPEKPADVAIDFAGITGRVLGFPVDEGDYDRIVAVR
                     DRVLFTRFPMRGIKPARREDSDNERHGALLAYDFEQQRLATIASECDDLRLAGDGRTL
                     VYRFGERLRAIDALLDLPEDGDEQKPPAEAGRKSGWIDLDRASVEIVPRNEWAQMYRE
                     AWRLQTEQFWVEDMSDIDWDRVYDRYRAVLPRVRTRGELSDLIWEMQGELGTSHAYEY
                     GGDYREPPQYQRGFLGADLVWDGDRNGYRIERIYRGDSWNREIDSPLAEPGLAIAEND
                     VIVAIGGKRVSENVSPDRLLVNA"
BASE COUNT          996 a         2059 c         1924 g          988 t
ORIGIN      
        1 ggccgtcgga cgatccggtg acgtaccgct acaacgttcc gcaaaacgcc tttgcggtga
       61 tcgcgatgcg cctgctggcg cgcttcgcgc gcgaagcgtt cgacgatccg aagctggccg
      121 ccgacgccac caccctcgga aatcaggtcc aagtcgggat cgaacgctac ggcaagacgt
      181 gggatccgac ccgcgggtgg acgtacgtct acgagaccga cggctacgga aacgacaacc
      241 tgatggacga cgccaacgtt ccgaatctca cggcgctgcc gtatctcggc tggtgcgcca
      301 ccgacgatcc ggtctacctc gacactcgcc ggttcacgct cagcttctcg gatccgtact
      361 actacaccgg taagtatgcg accggcctcg gaagcccgca cacgcccaag ggctgggtat
      421 ggccgctcgg cattatcggc gccgccctct cgacgcgccg ccgccaagag atcgcgcaag
      481 ccatcgacat gctcgatcaa agcgacacgc tcaacggtct catgcacgaa tcggtcgatc
      541 ccaacgatcc gtcgcgcttc acgcgacccg agttcggatg ggcgaacgca ttttgggccg
      601 acttgctctt ccggaccgtg gcgggatacc gcggaatccc gtacgtaacg gtcaacaccg
      661 tggttccact cgaacaggtc agcgaaatcc cgacgattac gccgctgtcc gtccagcttg
      721 ccgacacggc ggagctgaac tcgacgctcg gatatttgct gagtacgcag cggccgccgt
      781 agcgtgcacc tgctgctcgc ggcgctgctc acgcttccgc ccggcgtccg gtcggcggtc
      841 gacgcgaccg tcgcgcgcgt catggcggaa cggcacgtcg ccggattgtc gctcggcatc
      901 gcgcgcgccg ggaccacgct gtacgtgcgc ggatacggcc tgcgcgacgt cggcggccgg
      961 ctcgccgcac gcggcgacac gccctatccc gtcggctcga tcgccaagca attcgtcgcg
     1021 gccctgatac tgcaggacgc agccgccggc acgatcgcgc tcgacgcacc gctgagccgc
     1081 tacctgcccg cggcgagtac ggacgcggcg accgtcgcgc aagtgctcgg ccagaccggc
     1141 gggatcgccg cggatgccgg cgatctgcgc gcgccggggc aaacgctcgt cgaagcgatc
     1201 gccggcgccc cgccggcggc ggctccgggc acggcctgga cgtacagcaa cgccaactac
     1261 gtgctgctgg aagcggcgct cgaacgcgtc gatggggtgg gctttgcgga tctgctgcgg
     1321 cggcgaatcg tcgaaccgct cgggttaggc tctaccggct acggcattcc ggccggcggc
     1381 gttgcggcgc tcggatacgc gtgggacggc ggttggcacg cggtcgcacc gtccggcgtc
     1441 gcggacgatc ggacgcggct cgccggcggg atgtattcga gcgtaccgga cttgctgcgc
     1501 tggctcgacg cgctgcgttc cggacgcgtc gtgacgcccg accgtttcgt tgcgatgacg
     1561 tcgtcgggga aactctccgg cggcatcccc acgaactacg gcttcggatt cttcatcgcc
     1621 gactggttcg gataccgggt cgcggaacac cccggctacg tcgacggctt ctccgcacag
     1681 gacgcgctcg tcttggacga cggactcgag gtcgcggtgc tcgccaacgg catggccgtc
     1741 gacttgacgc cgttaacgat gagcctggtc gcggcgatcg atcgcccgcg cgacgccaat
     1801 ctcgcggcgc tcccgtcgca gccgccccaa aacgagaatc tcgcgatcac ggccgacgtg
     1861 aaggcactcg tcgaaacgcc cggattcgcg gcgctcggag cgctgcagtc gatcgaattc
     1921 gtcgagcggt cgctcgcggg cggcacgacg tacgacaagt atcggctgac gttttccacc
     1981 ggccagtggt gggtaacggt cggctatcgt gaaaacggcc cgatcgaggc gctgacgtta
     2041 tcgccggtgc aatgacgcat tacctgcgcc tcggtgcgat ctgggtgctg gccgcgatgg
     2101 cggcgtggtc cgttcccgct ccgtgcccgg ctcaagtgct gatcgtgccc ctcaccggca
     2161 agcgcgtgca cgacgttcaa gccgcgcagc tctttcacac gctgttcaac gacttctttc
     2221 tcgaagacga cgccaccacg tacgtccaaa ccggcgacat tccggcgatg tggctgcgcg
     2281 actcgtcggc acaaacgatt ccgtacgtgc ggtttcaaac ggcctttccg attctgcgcg
     2341 gacggttcgc cggcgtaatc gagcggaacg cgcgcaacat cgtcacggat ccgtacgcca
     2401 acgcgtttca ggccgactac cacgtttggg aacgcaagtg ggaagtcgac tcgctcgcct
     2461 ggccgatggt tttgacgtgg gtgtactggc gcgtaacccg cgaccgcacg atcttcaccc
     2521 ccgagctgca cgtcgcgatg cagaagatcg tctcgaccta tctttgcgag gagcgacaca
     2581 gccgctgcaa cgcctaccaa tacccgtatc gcgttcgaac caactacgcg tacgcggccg
     2641 gcaccggact catttgggga gcgtttcggc cgtccgacga tcccgtgcag ttccggttca
     2701 acattccgca gaacgcgatc gcggtggtcg cgctgcacga aatcgagggg ctcgcgctcg
     2761 acggatacgc cgaccgcgat ctcgcgcaga gcgcgcgcgc gctagccgct cgggtccagg
     2821 tgggcgtcga acgttacggc cgcttcttcg atgccgggcg ccgccaatgg atgtacgcgt
     2881 acgaaaccga cggattcggc cgctacaacc tgatggacga cgccaacctg ccgaacatca
     2941 cgaccttgcc ctacatcgac tggtgttcga atctcgaccc gacctatcgc aactcgcggg
     3001 cgttcgttca aagtaagaac aatccgtact tctactcggg acgctacgcg gccgggctcg
     3061 ggagcccgca cacgccgccg cgatacgttt ggccgctcgg catcatcggc ggcgcgctca
     3121 ccgcccgcag ttccggcgac gtcgccgaag cgatcaccac gctggcgatc accgacggcg
     3181 agtcgggcac tattcacgag agtttctacg atgacggcta ctggcggtac acccgcggcg
     3241 aattcggctg ggccaacgcg ctcggcgcgg agctgctgtt tcgaagcttg gccggatatc
     3301 cgtcgaccca gttcgacccc ggcggcccgg tgctgccgtt tcagagccgg acccagacgc
     3361 ccaccctcgt gcttcctttt acacaagcgg aaaacgccgg caagatcgtg gcggcgctgg
     3421 ccggcctgct gcacgtcgag ggcacgatgc cccacctgcg gtcgaggtag ccgccgaccg
     3481 catcgccaag gaccctcctc gacatgaacc agggatacta tcgctatcca acgatagccg
     3541 gcgaccgcat cgtcttcgtg tgcgaggacg acctgtggag cgtccccgcc gccggcggcg
     3601 ccgccacccg cctgacggcc agcttcggcg cctgctcctt cccgcggctc gcgcccgacg
     3661 gggagtcgat cgccttcgtt tcgaccgacg agggcagccc ggaggtctat gtcatgccgg
     3721 cggccggcgg cgtccccgag cggctgacgt tcctcggagc cgtgctggcc aacgtcgtcg
     3781 gctggtcgcc cgacggcagc gacatctact tcgtcgccaa tccgacctcg tgggacgacc
     3841 agccgcgtcc gtttgcgatc gcgcgcgacg ggggaacgcc gcgtccgctc gagctcggcc
     3901 acgcgcgggc gttcgcgcgc ttcggagaaa acggcacggt gatcgggcgc aacgccgccg
     3961 atccggcgcg ctggaagcgc taccgcggcg gaaccgccgg cgaggtctgg atcgacgccg
     4021 gaggtaccgg cacgttcgca cgcatccccc tacccaacgg caatcccacc tggccgatgc
     4081 cgatcgggga ccgcgtctac ttcctagccg atcacgaggg catcgggaac atctactcgt
     4141 gcgagttcga cggcgccgac cttcgccgcc acacgcacga ggcggagtat tacgcgcgct
     4201 ttccgtctac ggacggcacg cgcatcgtgt attccgccgg cggcgagatc ctttgtctcg
     4261 accttgccga ggatcgcgta acgcgcgtac cggtcgcgac caactcgccc gctccgcaga
     4321 ccgtacgccg gttcgaagac gcctccgaat cgctcgagca tttcgcgccc catccggacg
     4381 gcacgcggct cgccttcgtt tcgcgggggc aggccttcac gatgcccttt ttcgacggcg
     4441 ccgccgtcgc atacggcacc ggcggtacgg ggcgcgtccg gcacgcggag tggctgtgcg
     4501 acggcaaacg cttcgcgtgc gtgaccgacg tcaacggcta cgagcagatt gcggttcagc
     4561 gcgccgagcc cgcgaacgaa gcgccggagc tggtcaccac cggcgacatc ggccgcgtca
     4621 ccgatttgac gtgctcgccg gtcgccgacg tcgtcgcctt cgccaaccac cgccacgagc
     4681 tgtgtttgct ggatctcgac gacggcagcg tgcgggtcgt cgaccgcagc ccggcacacc
     4741 gcatcggcga tctcgcgttc tcgcccgacg gacgattcat cgcgtacgtg tggtggccga
     4801 cgcaaggaac gtcgatcgtc cgggtcgcga aaatcaaatc ggggaaggtt cacgacgtgt
     4861 cgtcgccgct gcggatcgac caatcgccgg cgtgggaccc ggacgggaag tacctgtact
     4921 tcatttcgac gcgcgatttc aatccggtct acgacgcgtt gcagttcgag ttgagctttc
     4981 cgcaggccag ccgtccgttc gtcgtgacgc tgcgcgacga cgtcgcctcg ccgttcgtgc
     5041 cgcctcccaa accggtgcac cgcgaccacg agcacgatcg ggacgatcgc aaaaagccgg
     5101 agaagccggc cgacgtagcg atcgatttcg ccggcatcac cggacgcgtg ctcggcttcc
     5161 cggtggacga aggcgattac gatcgcatcg tcgccgttcg cgatcgcgtg ctgtttacgc
     5221 gcttcccgat gcgcggaatc aagccggctc gccgcgaaga ctccgacaac gagcgtcacg
     5281 gcgcgctgct ggcgtacgac ttcgagcagc agcggctcgc gacgatcgcc tcggagtgcg
     5341 acgaccttcg cttagccggc gacggccgga cgctggtcta tcgcttcggc gaacggctgc
     5401 gggcgatcga cgcgctgctc gatcttcccg aggacggcga cgaacagaaa ccgccggccg
     5461 aagcggggcg caagagcggt tggatcgacc tcgatcgcgc cagcgtcgaa atcgtcccgc
     5521 gtaacgagtg ggcgcaaatg taccgcgagg cgtggcggct gcagaccgag cagttctggg
     5581 tcgaggacat gagcgatatc gactgggacc gcgtgtacga tcgctatcgc gccgtgcttc
     5641 cgcgagtgcg tacccgcggc gagctttcgg atctcatttg ggaaatgcaa ggcgagctcg
     5701 gcacgtcgca cgcctacgag tacggcggcg actaccgcga gccgccgcag taccagcgcg
     5761 gatttctagg cgccgatctc gtgtgggacg gagatcgcaa cggttaccgc atcgagcgta
     5821 tctaccgcgg cgattcctgg aaccgcgaga tcgactctcc gctggccgaa cccggcctgg
     5881 cgatcgccga aaacgacgtg atcgtcgcga tcggcggaaa acgggtttcg gagaacgttt
     5941 cgcccgatcg tctgctggtc aatgccg
//
LOCUS       DASUCJ010001163         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1173913, whole genome shotgun sequence.
ACCESSION   DASUCJ010001163 DASUCJ010000000
VERSION     DASUCJ010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173913"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFU90_13675"
     CDS             <1..605
                     /locus_tag="VFU90_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5480889.1"
                     /translation="PAGYRIDWDGEWHMTATVFADLGRAMLLAFVLIYFVLVARFRSF
                     RVPLVVLAAVPLAFIGIMPGFALLAPFGVYFSATAMIGLIALIGIVVRNSIILIEFIE
                     ERRAQGAGLHQALVESAAVRTRPIFLTAAAGVLSSIVIASDPVWSGLAWSLVFGMTTS
                     AVLSVLAIPLLYRAVAPERAETATPARDGGAALAPEAARA"
     gene            598..1884
                     /locus_tag="VFU90_13680"
     CDS             598..1884
                     /locus_tag="VFU90_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5480890.1"
                     /translation="MRKAIALSTFLALMLPVPLAAATAQTLTLDAAIATALRANPSYR
                     EALVAVDADAARVREARAPLGPSLTVSDAYQYQDVVAKLATPFGPLPFSPNSTNLPLL
                     AANYTLFDGGETAARLASASATLAASQSSARSARASLIVAVSAAYFDLAAALLGADVA
                     DHSVSVAQAHVDLARRRLTAGQIPRAELLQAQADLAGKRVDALTAHNAAAIDQNRLDA
                     VMNVELGTQHDPVDRLETNVPDLDLDELIAAARERRGDLAAAELAVDASRHAVAAARA
                     QRAPRIALAASEGNVQPIVVPGFHAQFTVGLTAVWQLFDNGYTDGRVAEAQSAVSQAQ
                     LELMRLQTDAELEVRQAYLNLQTARLQVAAAERLVSVTAENQRLAEVRYRGGVGAAIE
                     LRDAELRDDSARQSLIDALRSQRESLVSVRFAAGFL"
     gene            1914..2336
                     /locus_tag="VFU90_13685"
     CDS             1914..2336
                     /locus_tag="VFU90_13685"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5480891.1"
                     /translation="MTTPASEASLTYGRAVSTGLPFAEAVERTRVQLKEEGFGILCDI
                     DVAATMREKLGESFRPYRILGACNPQLAHRALEAQPYLGLLLPCNLIVQESDGRTLVA
                     AVDARAMLGVVGDPQLSALADEVNARLGRVLDAIASAS"
     gene            2333..3760
                     /locus_tag="VFU90_13690"
     CDS             2333..3760
                     /locus_tag="VFU90_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5480892.1"
                     /translation="MKPRVLVLGAGFAGHTAALHLSRLVRGKADVTVVSPGNRFTWFP
                     SLVWVGNGTMDPDEVHFELAPVYQRIGVDYVDGRATAVKPDDRQVVVQTASGSPTTLT
                     YDFLVNATGPYLNFEGTPGLGPATGNTTSICSVEHAVDARDKYLAIVRDLEAGKRRRI
                     VVGVGHPAATCEGAAFEYLMNVNADLRERRLRERAELVWLTNEPEAGDFGVDGIEAIK
                     HGTLVTGAALVRMLLDEAGIVPKIGAGVTKVEPGLLHYAQVGEEPATLEYDLAMLIPQ
                     FRGIPMRYVGADGADIAAEMTVPSGFMRVDADYGPKTYEQYRAADWPAKYQSPRYENV
                     YAAGIAFAPPHPMSKGKKAGDGLTIQATPPRTGMASGIMGRTVAENIAEQVNGKTPSH
                     HARMSEMPAACIASVGKSVWTGSAASILMTPVARDYERYPAHGRDLSLCDLDVGLAGA
                     WTKRALHSAFLWKLQGKPGWELIPE"
BASE COUNT          627 a         1247 c         1293 g          667 t
ORIGIN      
        1 tccccgccgg ctaccgtatc gattgggacg gggaatggca catgaccgcg acggtcttcg
       61 ccgatctcgg ccgcgcgatg ctgctggcgt tcgttctcat ctacttcgtc ctggtcgcac
      121 ggtttcgatc gtttcgcgtt ccgctggtcg tgctggccgc cgtgccgctc gccttcatcg
      181 gcatcatgcc cggattcgcg ctgctggcgc cgttcggcgt gtatttttcc gcaaccgcca
      241 tgatcgggct gatcgcgctg atcggtatcg tcgttcgcaa ctcgatcatt ctcatcgagt
      301 tcatcgaaga acgacgagcc cagggcgccg gcctgcacca agcgctggtc gagtcggcgg
      361 ccgtccggac gcgtccgatc tttctaacgg cggcagcggg cgtcttgtcg tcgatcgtca
      421 tcgcctccga tcccgtgtgg agtggtttgg cgtggtcgct ggtgtttggg atgaccacgt
      481 cggcggtgct ttcggtgttg gcgattccgc tgctctatcg cgccgtcgcg cccgagcgcg
      541 ccgagacggc gacgcccgcg agagacggcg gggcggctct cgcaccggag gcggctcgtg
      601 cgtaaggcaa tcgcgctgtc gaccttcctt gcgctgatgc tgccggtacc gcttgcggcc
      661 gcgacggcgc agacgctcac cctcgacgca gcgatcgcga cggcgctacg cgcgaatccg
      721 tcgtatcgcg aagcactggt cgcggttgac gccgacgccg ctcgcgtgcg ggaagcgcgc
      781 gcgccgctag gcccgtcgct gacggtctcc gacgcgtatc agtatcaaga cgtcgtcgca
      841 aagctcgcaa cgccgtttgg tccgctgccg ttctcgccca actcgaccaa tctaccgctg
      901 ctggcggcaa actacacgtt gttcgacggc ggcgaaacgg cggcacgctt ggcgtcggct
      961 tcggcgacgc tggccgcgtc gcaatcgtcg gcccgcagcg cacgcgcgtc gctgatcgta
     1021 gcggtttcgg ccgcctactt cgatctcgcg gcagcgctgc tgggtgcgga cgtggcggac
     1081 cattcggtct cggtcgcgca ggcgcacgtc gatctcgcgc ggcggcggct gacggccgga
     1141 cagattccgc gcgccgagct gctgcaggcg caagccgacc tcgccggtaa gcgcgtggac
     1201 gcactgacgg cgcacaacgc ggcggcgatc gatcaaaacc ggctcgatgc cgttatgaac
     1261 gtcgagctgg gcacgcagca cgatccggtc gaccgtctcg agacgaacgt ccccgacctg
     1321 gacctcgacg aactgattgc cgcggcacgc gaacgccgcg gcgatctcgc cgccgccgag
     1381 ctggccgtcg acgcgtcgcg ccatgcggtc gcggcggcgc gcgcgcaacg cgcgccgcgc
     1441 atcgcgttgg cggccagcga gggcaacgta cagccgatcg tggtaccggg ttttcacgcg
     1501 cagttcacgg tcggcttgac ggccgtctgg caactcttcg acaatgggta cacggacggc
     1561 cgagtcgccg aggcgcagag cgcggtttcg caagcgcagc tcgagctgat gcggctccag
     1621 accgacgccg agctggaagt gcgccaggcc tatctcaacc tccaaacggc tcgcctgcag
     1681 gtcgcggcgg ccgaacggct cgtcagcgtt accgcagaga accagcggct tgccgaggtg
     1741 cgttatcgcg gcggcgtcgg cgcggccatc gagctgcgcg atgccgagct tcgcgacgac
     1801 tccgcccggc agtcgctcat cgacgcgctg cgttcgcagc gcgaaagttt ggtatccgtc
     1861 cgctttgcgg ccggatttct ctaacccgca ctccgtcccg aaagggggca atcatgacaa
     1921 ccccggcttc cgaagcatcc ctcacctacg gccgcgcggt cagcaccggt ctcccgtttg
     1981 ccgaagcggt ggaacgcacg cgcgtgcagc tgaaagaaga aggtttcggc atcttgtgcg
     2041 acatcgacgt cgcggcaacg atgcgcgaaa agctcggcga atcgtttcgc ccgtatcgca
     2101 ttttaggcgc gtgcaacccg cagctggcgc atcgggcgct ggaagcgcag ccatacctag
     2161 gacttctgct gccctgcaac ctgatcgtcc aagagtcgga cgggcggact ctggtcgccg
     2221 ccgtcgacgc tcgcgcgatg ctgggcgtgg tcggcgatcc tcagctgtcg gccttagccg
     2281 acgaagtcaa cgcccggctc gggcgcgtgc tcgacgcgat tgcgagcgcg tcgtgaaacc
     2341 gcgagtgctg gtgttgggtg ccgggtttgc cggccatacc gccgcgctgc atctttcgcg
     2401 actcgtgcgc ggcaaagccg acgtcaccgt cgtatcgccc ggcaaccggt tcacgtggtt
     2461 tccgagtttg gtgtgggtcg ggaacggcac gatggacccg gatgaagtcc acttcgagct
     2521 ggcgccggtt taccaacgca tcggcgtcga ctacgtcgac ggccgcgcga ccgccgtcaa
     2581 accggacgac cggcaagtcg tcgtccagac cgcgagcgga tcgccgacga cgctcacgta
     2641 cgactttctc gtcaacgcta ccgggccata cttgaacttc gaaggaacgc ccggacttgg
     2701 ccccgcgacc ggaaacacta ccagcatctg ctcggtggag cacgccgtcg acgcgcgcga
     2761 taagtatctg gcgatcgtgc gggacctcga ggccggcaaa cggcggcgaa tcgtcgtggg
     2821 cgtggggcat ccggcggcga cgtgcgaagg cgcggcgttc gagtatctca tgaacgtcaa
     2881 cgcggacttg cgcgaacggc ggctgcgcga acgcgccgag ctggtctggc tcacgaacga
     2941 acccgaagcc ggcgatttcg gcgtcgacgg catcgaggcg atcaagcacg gaacgctggt
     3001 gaccggcgcg gcgctggtac gaatgctgtt ggacgaggcg gggatcgtgc cgaagatcgg
     3061 cgccggcgtg acgaaggtcg aaccggggct gctgcattac gcgcaggtcg gcgaagagcc
     3121 ggcgaccttg gagtacgacc tcgcgatgct gattccgcag ttccgcggca ttccgatgcg
     3181 gtacgtcggc gccgacggcg ccgacatcgc agcggaaatg accgttccga gcggtttcat
     3241 gcgcgtcgac gcggactacg ggccgaagac ctacgagcaa tatcgggctg cggactggcc
     3301 cgcgaaatat caatcgccgc gttacgaaaa cgtctatgct gccggaatcg cgttcgcgcc
     3361 gccgcatcct atgtcgaaag gcaagaaagc aggcgacgga ctcacgatcc aagcgacgcc
     3421 gccgcgcacc ggaatggcgt cgggcatcat ggggcgcacg gtcgccgaaa acattgcgga
     3481 gcaagtcaac ggaaaaacgc cctcgcatca cgcgcgcatg tcggagatgc cggcggcctg
     3541 catcgcttcg gtgggcaagt cggtatggac cgggtcggcc gcttcgatac tcatgacgcc
     3601 ggtggcacgg gactacgagc gttatccggc gcacgggcgc gatctctcgc tatgcgatct
     3661 ggacgtcggt ttggccggag cgtggacgaa gcgcgcgctg cattcagcgt ttctgtggaa
     3721 acttcagggt aagccgggct gggaactcat tcccgaatag gaggatagac agtgggtacg
     3781 ttttggcggc gattttggcc gtatcaaatc tggcgcttcg tcgtgatcaa tacc
//
LOCUS       DASUCJ010001164         1434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_471060, whole genome shotgun sequence.
ACCESSION   DASUCJ010001164 DASUCJ010000000
VERSION     DASUCJ010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471060"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFU90_13695"
     CDS             <1..776
                     /locus_tag="VFU90_13695"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5480893.1"
                     /translation="FTIDEFLSIPLDFAYVPLCQTWPVAAPAHPPGQPIPPGAGMPSV
                     PALVLTGDLDTITTPAEGDATAQLFRDARRVIVVNTGHVTAVGDVYACASAIVRDFIA
                     RRFVDARCAGAVPPMRLVPAFARSAAGVPPATATVPGESDGDLRLAADATAAAGDALA
                     RVVDLGMTRGSGLRGGSVVARSDAAGTRLSLQGVEWTEDLAVSGDVAFDSHTGAVGAR
                     LRWSGAALQVEWRSYAPSAQATIVGTIGGRRIRATMPAP"
     gene            833..>1434
                     /locus_tag="VFU90_13700"
     CDS             833..>1434
                     /locus_tag="VFU90_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480894.1"
                     /translation="MTFSRKDFIAVSALGASVATVADLAAPAAAQARAPVHFEILKPD
                     EFDGAAMLRTLSPDSAHKQIFQTSTPLVVAPGIASIYLHMQNAMNAYQFSLGLGKLST
                     LAVLMGPAIVLALNDATWTKYRFGKGLGAKIGLADTNVYYTAASNLDFGAQPDDPNGV
                     YQDWSAQAVLKRGGRFFVCHNAMSAIALVTTMESGGDPKAV"
BASE COUNT          218 a          501 c          475 g          240 t
ORIGIN      
        1 cgtttacgat cgacgaattc ttgagcatac cgctcgattt cgcgtacgtg ccgctatgcc
       61 aaacctggcc ggtcgccgca ccggcgcatc cgccgggaca gccgataccg cccggtgccg
      121 gaatgccgag cgtcccggcg ctggtgctca ccggcgatct cgacacgatt accactccgg
      181 ccgaaggcga tgccaccgcg cagctgtttc gcgacgcgcg ccgcgtgatc gtcgtcaaca
      241 ccgggcacgt taccgcagtc ggcgacgtct acgcgtgcgc gtccgcgatc gtgcgcgact
      301 tcattgcgcg ccgcttcgtc gacgcgcgct gcgccggcgc cgttccaccg atgcgcctgg
      361 tcccggcgtt cgcccgcagc gccgccggcg tgcccccggc gaccgcgacc gtcccgggcg
      421 agagcgacgg cgatctccgc cttgctgccg acgcgaccgc cgccgccggc gacgcgctcg
      481 cgcgcgtcgt cgatctcggg atgacgcgcg gcagcggcct gcgcggcggt tcggtcgtcg
      541 ctcgatcgga tgcggccggc acgcggctgt cgctccaagg cgtggagtgg acggaagatc
      601 ttgccgtctc gggggacgtc gcattcgatt cgcacaccgg tgcggtcggg gcgcgtttgc
      661 gctggagcgg cgccgcgctg caggtggagt ggcggtcgta cgcgccctcc gcgcaggcca
      721 cgatcgttgg aacgattggg ggacgccgta ttcgcgcaac catgccggcg ccgtaacccc
      781 gtaggacttc gcggaccaac gggataagcg cccgtttgaa gggagctcgc ccatgacgtt
      841 ctcacggaag gactttattg ccgtctccgc gctcggcgcg tcggtcgcga cggtcgccga
      901 tctggcggcg cccgcggccg cccaagcacg cgctccggtc cacttcgaaa ttctcaagcc
      961 ggacgaattc gacggcgcgg cgatgctgcg tacgctctcg cccgattcgg ctcacaagca
     1021 gattttccaa acgtcgacgc cgttggtcgt cgcaccgggc atcgccagca tctacctcca
     1081 catgcaaaac gcgatgaacg cgtatcagtt ctcgctcggg ctcgggaaac tgtcgacgct
     1141 cgcggtgctc atgggcccgg cgatcgtcct cgccctcaac gatgcgacct ggacgaaata
     1201 ccggttcggc aagggtttag gcgccaagat cggcttggcg gacacgaacg tctattacac
     1261 cgcggcttcg aatctcgatt tcggcgcgca gcccgacgat cccaacggcg tctaccagga
     1321 ttggagcgcg caggcggtac tcaaacgcgg tggccggttc ttcgtgtgcc acaacgcgat
     1381 gagcgcgatc gcgttggtca ccaccatgga gagcggaggc gacccgaagg ccgt
//
LOCUS       DASUCJ010001165         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_623470, whole genome shotgun sequence.
ACCESSION   DASUCJ010001165 DASUCJ010000000
VERSION     DASUCJ010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623470"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>4055
                     /locus_tag="VFU90_13705"
     CDS             44..>4055
                     /locus_tag="VFU90_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEU5480895.1"
                     /translation="MTLETQPTPDPGGGAAELFDPSLKTWVYDLTPQRDLHVARSYSL
                     KIEPGVEPAYGNVASADAFAGAIRTYDALSIVPTPVPSPGANGGGRFADGDPAIVFSN
                     PLDPKSIAGAVSVSPAPANVKALTSLSDDATTISIDPYALDPDATYTVTVAPGVKDVF
                     GQTLGQSQTVTVHTGDFAPGAWSPTGSSVIPAGLGVALNFYATNLPANRYQAAYARLS
                     PDRLFGYPDPLTDLPAYASWPQRTLAGARRNVQSVVRIPLQQQTGGPFGAIAYGFRTA
                     LDASDGSPSLVGTAQLTNLGISAELFPARGFVLVQHLSDGAAAAAVKVTLYRNLQTAS
                     VPPAVCASATTGAGGEADFGLVDLERCFAGTAANETPTVGVIATEGGDAATLTLNGSD
                     GLYRFAVNPGWSSGAPLSRGIVFPDRDMYQPGERGELTGIAYSVSGSRVVADRNAVYR
                     VTLSDPSNAVTRLSDVKTDAYGIFSLPIVFSKQQALGYYTVDAKGASGNDINGSLRVA
                     EFKPPNFKLTVDLSATSATAGGSIHAAATAAYLFGAPLQGGNLHAYVTRESATVAPKG
                     WDDFWFGRQWFWPEQTPSFDTDVLQRDLPLDAQGKSSLDVTVPNDLPFPMTYTVDMEA
                     TDVSNLSVADSQNFLALPAGAAIGLASDVVGTAGKPMPIRAIVTDADGRAIAGRSLHF
                     ELQKMTYTSATQEVEGGESAQQAVKYDTVATADATSDAKPVTVNLTPSDSGPYRVRAN
                     FSDAKGDASATDIQVFAFGSGEADWRQSDTTVVNVELDKKAYAVGQTATALVASPFDR
                     SDVYVSVVRGDALYRTTLHDVHGAARVAFKITPDMLPNAAFQAVVVRRGADIAKLKNA
                     MPDALARTGMAAFDVDVADRYLKLGIAPQAATVAPGGTQRVTFTLSRKDGAAAQGEIV
                     AMVVNDAILQLSGYRLPDLVTTVFADQPISAMLGDSRDGAVLKTQTPPLEKGYGYGGG
                     YLAGAAGTRVRTQFLPMAYYGAVKTDASGKASASFKLPDDLTTWRVMAVAVGSDDAHF
                     ATSDSTFVATLPLLANPLLPQFARTGDTFDLGVAFSNQTGAGGALDLVMKLTGSLAFA
                     GGDPRAKTAQEQAATGMQAYRFPVTVGTPAPTTVEASGKLGSHGDAFQVPFTVSNRTS
                     TDSVIESGAVTSSTATVPIDLSRGGWLTLTLANSIVPEFAVPSAAMMAQDGLPLADET
                     ASRLTIGSALAALRTPYRLKLQFDPNAESAKDLQQLLQFQRGDGGFGETAGARESDPF
                     VSTYALDALFFAKARGVAIDPAALSRARAFTAAVLANPGRFRWCANDRICKAQLRFEA
                     LWALAQGGDRRTDFLSD"
BASE COUNT          671 a         1388 c         1349 g          647 t
ORIGIN      
        1 ccatacgagc ctggttggcg acggtgaaag catcccggta aacgtgacgc tcgaaacgca
       61 gccgacgccg gacccgggcg gcggcgcggc cgaactgttc gacccgtcgc tgaagacctg
      121 ggtgtacgat ctgacgccgc agcgcgatct gcacgtcgcg cgttcgtatt cgctcaaaat
      181 cgagccaggc gtcgagcccg cctacggcaa cgtggcgtcg gccgacgcgt tcgccggtgc
      241 gatccgtacg tacgacgcac tctcgatcgt tccgacgccg gtgcccagcc ccggcgctaa
      301 cggcggagga cgcttcgccg acggcgaccc ggcgatcgtg ttcagcaacc cgctggatcc
      361 gaaatcgatt gccggcgccg tcagcgtttc gccggccccg gccaacgtga aggcgttgac
      421 ctcgctctcg gacgacgcca cgacgatttc catcgatccg tacgcgctgg atccggatgc
      481 aacctatacc gtcacggtcg cccccggcgt gaaggacgta ttcgggcaaa ccttggggca
      541 gtcgcagacg gtgacggtgc acaccggcga tttcgctccc ggcgcgtggt cgccgaccgg
      601 ctcgagcgtc attccggcgg gtctcggcgt cgccctgaac ttttacgcga ccaaccttcc
      661 cgcgaatcgc tatcaagccg cgtacgcgcg gctctcgccg gaccgactct tcggctatcc
      721 ggatccgctc accgatttgc cggcgtacgc gtcgtggccg cagcgaacgc tggccggcgc
      781 gcggcgcaac gttcaaagcg tcgtgcgcat tccgctgcag cagcaaaccg gcggcccgtt
      841 cggcgcgatc gcgtacggct tccggaccgc gctcgacgct tccgacggtt cgcccagcct
      901 ggtgggcacg gcgcagctga ccaatctcgg catttcggcc gaactctttc cggcgcgcgg
      961 cttcgtgctg gtgcagcact tgagcgacgg cgccgcggcc gcggccgtga aggtgacgct
     1021 ctaccgaaac ctccagacgg cttcggtgcc gccggcggtc tgcgcttcgg ctacgaccgg
     1081 cgccggcggc gaagccgatt tcggcctggt cgatctcgag cgatgctttg ccggaaccgc
     1141 ggcgaacgaa acgccgaccg tcggcgtgat cgcaaccgaa ggcggcgacg ccgcgacgct
     1201 gacgctgaac ggatcggatg gcctctatcg ctttgcggtg aatcccggct ggtcgagcgg
     1261 cgccccgctt tcgcgcggca tcgtgttccc ggaccgcgac atgtatcagc ccggcgagcg
     1321 cggcgagctg accgggatcg cgtactccgt gagcggcagc cgggtcgtcg ccgaccgcaa
     1381 cgcggtctat cgcgtgaccc tgagcgatcc cagcaacgcg gtaacccggt tgtccgacgt
     1441 gaagaccgac gcgtacggga ttttctcgct gccgatcgtc ttttccaaac aacaggcgct
     1501 cggatattac accgtcgatg ccaaaggcgc gagcgggaac gacatcaacg gctcgctgcg
     1561 cgtcgccgag ttcaagccgc cgaacttcaa gctgacggtc gacctgagcg ccacgtcggc
     1621 gaccgcgggc ggcagcatcc acgcggccgc gacggccgca tatttgtttg gcgcaccgct
     1681 gcagggcggg aacctgcacg cctacgtcac ccgcgagtcg gcaacggtcg ccccgaaggg
     1741 ctgggacgat ttctggttcg ggcggcaatg gttctggccc gaacaaacgc cgtcgttcga
     1801 caccgacgtg ctgcagcgcg acctgccgct cgacgcgcaa gggaaatcga gcctcgacgt
     1861 gaccgtgccg aacgacttgc cgttcccgat gacctacacc gtcgacatgg aagccaccga
     1921 cgtatcgaac ctttcggttg ccgattcgca gaacttcctc gcgctgccgg ccggtgccgc
     1981 gatcgggctt gcgtccgacg tcgtcggaac ggccggcaaa ccgatgccga tccgggcgat
     2041 cgtcaccgac gccgacggcc gcgcgattgc cggccgctcg ctgcacttcg agctgcagaa
     2101 gatgacgtat acctcggcga cgcaggaagt cgaaggcggc gaaagcgcgc agcaggcggt
     2161 gaagtacgac accgttgcga ccgccgacgc gacttcggac gcaaagccgg tcacggtcaa
     2221 ccttacgccc tccgatagcg ggccgtatcg cgtgcgcgcg aacttcagcg acgcgaaagg
     2281 cgacgccagc gcgaccgaca ttcaagtgtt cgcgttcggg tcgggtgaag ccgattggag
     2341 acagtcggac accaccgtcg tgaacgtcga gctcgacaag aaggcctacg cggtcgggca
     2401 gaccgcgacc gcgctggtgg cgtcgccgtt cgaccggagc gacgtgtacg tgtcggtggt
     2461 gcgcggcgac gcactctacc gcaccacgct gcacgacgtc cacggcgccg cgcgcgtcgc
     2521 gttcaagatt actcccgata tgctgccgaa cgccgcgttt caggcggtcg tggtacggcg
     2581 cggcgccgac atcgccaagc tcaagaacgc catgccggac gcgctcgccc gcacggggat
     2641 ggcggccttc gacgtggacg tagccgaccg gtatctgaaa ctggggatcg ctccccaggc
     2701 ggcgaccgtc gcacccgggg gaacgcagcg cgtgaccttc acgctgtcgc gcaaggacgg
     2761 cgcggcggcg cagggcgaga tcgtcgcgat ggtcgtcaac gacgccatcc tgcagctctc
     2821 cggttatcgc ttgccggatc tcgtgacgac cgtgtttgcc gatcagccga tctcggcgat
     2881 gctgggcgat agccgggacg gtgccgttct caaaacgcaa acgccgccgc tcgaaaaagg
     2941 ctacgggtat ggcggcggct atctggccgg cgcggccggg acgcgcgtgc gaacgcaatt
     3001 tcttccgatg gcgtactacg gcgcggtgaa gaccgacgcg agcggtaagg cgagcgcgtc
     3061 gttcaagctg cccgacgacc ttacgacttg gcgcgtcatg gcggtcgcgg tgggcagcga
     3121 cgacgcccac ttcgcaacct ccgattccac tttcgtcgcg acgctgccgt tgctcgccaa
     3181 cccgctgctg ccgcagttcg cacgcaccgg cgataccttc gatctcggcg tcgcattctc
     3241 gaatcagacg ggtgccggcg gagcgctgga tctcgtcatg aaactgaccg ggtcgctggc
     3301 ctttgccggc ggcgacccgc gcgcgaagac ggcgcaggag caagccgcga cggggatgca
     3361 ggcgtatcgc ttcccggtaa ccgtcggcac gccggcgccg accacggtcg aagcgtcggg
     3421 gaagctcggc tcgcacggcg acgccttcca agtgccgttt accgtgagca accgcacgtc
     3481 gacggattcg gtcatcgaaa gcggcgcggt caccagctcg acggcgaccg ttcccatcga
     3541 cttgagccgc ggcggctggc tgaccctcac gctcgccaac tcgatcgtgc cggagtttgc
     3601 cgttccgtcg gcagcgatga tggcgcaaga cggtttgccg ctggccgacg agaccgcatc
     3661 gcggttgacg atcgggtcgg cgctggcggc gctgcgtacg ccgtaccggt tgaaactcca
     3721 gttcgatccg aacgccgagt ccgcgaaaga cctgcagcag ctgctgcagt tccagcgggg
     3781 cgacggcggt ttcggcgaaa cggcgggcgc gcgtgaaagc gatccgttcg tcagcaccta
     3841 cgcactcgac gccctgttct tcgccaaagc gcgcggcgtc gcgatcgatc cggccgcatt
     3901 gtcgcgcgcg cgagccttca cggcggcggt gctcgccaat cccgggcgtt tccgctggtg
     3961 cgcgaacgat cgcatctgca aagcgcaact ccgattcgaa gcgctgtggg cgctcgcgca
     4021 gggcggcgat cggcgcaccg acttcttgag cgacg
//
LOCUS       DASUCJ010001166         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_195707, whole genome shotgun sequence.
ACCESSION   DASUCJ010001166 DASUCJ010000000
VERSION     DASUCJ010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195707"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1514)
                     /locus_tag="VFU90_13710"
     CDS             complement(51..1514)
                     /locus_tag="VFU90_13710"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5480896.1"
                     /translation="MTTTIATARKVPVTVAYGDGIGPEIMDATLRIITAAGAALDLER
                     IDIGESVYNKGLSNGIEPSSWESLRRTKVFLKAPITTPQGGGFKSLNVTVRKTLGMYA
                     NVRPCASLHPYVATKHPNMDLVIVRENEEDVYGGIEHRQTNQVAQCLKLISRPGSKRI
                     VRYAFEYARANKRKKVTCFTKDNIMKITDGLFHRTFDEIAREYPEIENEHWIVDIGAA
                     KMADTPEAFDVIVMPNLYGDVLSDVAAQITGSVGLAGSANIGDHCSMFEAIHGSAPRR
                     AGQNMANPSGLLHGALLMLVHVGQPEPAEKAHNAWLRTIEDGVHTYDVYKEGVSRQKV
                     GTREFADAVIARLGQKPQHLKPVEYAKNVHMNLAVRPGGTPPLKLRVGVDLFVDRPGG
                     DPDSIAAALQQLGVDGAKLTVISNRGTKVWPNGNPDTYWSDHWSCRFESDGTQPFSAQ
                     HVAELLSAAERAGFDVVKTEGLFTFDGQRGYSLAQGE"
     gene            complement(1567..>2002)
                     /locus_tag="VFU90_13715"
     CDS             complement(1567..>2002)
                     /locus_tag="VFU90_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480897.1"
                     /translation="VQQMYRPLGVEIQLKGYDYATLYAAAQSGGILNGGRFDLAMYSW
                     VSGADPDDSSQWTCAAIPPAGNNITRYCSPAMDAAQRLALSTFDRAVRKKAYSQIQEL
                     LLRDVPGAFIYYQALRYAHVAGLQNFTPNGISEGWNVQEWSR"
BASE COUNT          311 a          609 c          679 g          403 t
ORIGIN      
        1 tccttcgact gcgcgcctgc ggcgctccgc tcaggatgac accgcggtga ctactcgccc
       61 tgggcgagcg agtagccgcg ctggccgtcg aaggtgaaga gtccttcggt ttttacgacg
      121 tcgaagccgg cgcgttcggc ggcagacagc agctcggcga cgtgctgcgc ggaaaacggc
      181 tgcgtcccgt cgctttcgaa gcggcacgac cagtgatcgc tccagtacgt gtccggattt
      241 ccgttcggcc acaccttcgt gccgcgattg ctgatcacgg tgagcttggc gccgtcgacc
      301 ccgagctgct gcagtgcggc cgcgatcgag tccggatcgc cgccggggcg gtcgacgaac
      361 aggtcgacgc cgacgcggag cttcagcggc ggcgtcccgc cgggacggac tgcgagattc
      421 atgtgcacgt tcttggcgta ttcgaccggc ttgagatgtt gcggcttttg cccgagccgc
      481 gcgatgaccg cgtcggcgaa ctcgcgcgtt ccgacctttt gccgggagac gccctccttg
      541 tagacgtcgt aggtgtgcac gccgtcttcg atcgtgcgca gccacgcgtt gtgggccttt
      601 tcggccggct ccggctgccc gacgtgcacc agcatcagga gcgcgccgtg cagcaagccc
      661 gaggggttcg ccatattttg gccggcgcgt cgcggtgccg agccgtgaat cgcttcgaac
      721 atcgaacagt ggtcgccgat gttggccgaa ccggccagcc cgacggagcc ggtgatttgc
      781 gccgccacgt cggaaagaac gtcgccgtag agattcggca tcacgatcac gtcgaacgct
      841 tcgggcgtat ccgccatctt ggccgcgccg atgtcgacga tccagtgctc gttctcgatt
      901 tcgggatact cgcgcgcgat ttcgtcgaag gtacggtgga agagcccgtc cgttatcttc
      961 atgatgttgt ctttggtaaa gcaggtgact ttcttgcgct tgttggcgcg agcgtattcg
     1021 aaagcgtagc ggacgatgcg tttgctgccg ggccgcgaga tcagtttgag acactgcgcg
     1081 acctgattgg tctgacgatg ctcgattccg ccgtaaacgt cttcctcgtt ttcgcggacg
     1141 atcacgaggt ccatattcgg atgtttggtg gcgacgtagg ggtgcagcga cgcgcacggg
     1201 cggacgttgg cgtacatgcc cagcgtcttg cggacggtca cgttgaggct cttgaagccg
     1261 ccgccttgcg gcgtcgtaat cggcgccttg agaaacacct tggtccggcg cagcgattcc
     1321 cacgacgacg gttcgatgcc gttgctcagg cctttgttgt aaacgctctc gccgatgtcg
     1381 atgcgttcga ggtcgagcgc ggcgcccgcg gcggtgatga tgcgcagggt ggcatccatg
     1441 atttcggggc cgatcccgtc gccgtaggca acggtcacgg gcaccttgcg cgcggtcgcg
     1501 atcgtggtgg tcacgtcgtg gatctccttt agagcgtggg gcgaggtgcc gacgcttcga
     1561 cggtggctac cggctccact cctggacgtt ccagccctcg ctgatgccgt tgggggtgaa
     1621 gttctgcagc ccggcgacgt gggcgtagcg cagcgcctgg taataaatga acgcgccggg
     1681 aacatcgcgc aggagaagct cctggatttg cgagtacgcc tttttacgca cggcgcggtc
     1741 gaacgtcgag agcgccagcc gctgggcggc atccatcgcg ggcgaacaat agcgggttat
     1801 gttgttgccg gccggcggaa tcgcggcgca cgtccactgc gacgaatcgt cggggtccgc
     1861 gcccgaaacc caggaataca tcgcaaggtc gaagcggccg ccgttgagga tgccgccgct
     1921 ctgggcggcg gcgtagagcg tcgcgtaatc gtagcccttc agctggatct cgacgcccag
     1981 cggacgatac atttgctgca ct
//
LOCUS       DASUCJ010001167         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_256631, whole genome shotgun sequence.
ACCESSION   DASUCJ010001167 DASUCJ010000000
VERSION     DASUCJ010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256631"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFU90_13720"
     CDS             complement(<1..544)
                     /locus_tag="VFU90_13720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU5480898.1"
                     /translation="MTSRDARAALIRRIGSEIQDKRVLAAMQRVPRHSFMPGTPLRWA
                     YADTAAPIGYGQTISQPTIVAIMSEALELDGRERVLEVGTGSGYQAAVLSLLAAEVYS
                     IELVPELASSARARLERLGYANVHVRQGDGYQGWIEQAPFDRIVLTAAPPDVPDTLFD
                     QLRDGGLLVAPTGFTRSSQRL"
     gene            complement(541..849)
                     /locus_tag="VFU90_13725"
     CDS             complement(541..849)
                     /locus_tag="VFU90_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480899.1"
                     /translation="MNRLWRVASLLVCVVGPASACYVEAETAPIFVDGYEPLYYDGYV
                     VYYDELGRPFYYVNGGVVWIGPEYPGYGALVHHWRIYRPAYRHWYVNYGYRYRTYRRR
                     "
     gene            complement(987..>1281)
                     /locus_tag="VFU90_13730"
     CDS             complement(987..>1281)
                     /locus_tag="VFU90_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5480900.1"
                     /translation="DADRTVQPTLDALRRRATFHEIHGGLTADNALFEARRCMSCGNC
                     FECDNCYGVCPDNAVIKLGPGKRFRVDLDFCKGCGLCAAECPCGAIAMVPEEV"
BASE COUNT          244 a          419 c          411 g          207 t
ORIGIN      
        1 agagccgctg cgacgaacgc gtgaaccccg taggcgccac cagcaatccg ccgtcccgaa
       61 gctggtcgaa gagagtgtcg ggtacgtcgg ggggcgcggc ggtcaatacg atacggtcga
      121 acggtgcttg ctcgatccat ccttggtagc cgtcgccctg ccgcacgtgc acgttggcgt
      181 atccgagtcg ttctaggcga gcgcgcgccg agctggccag ctcgggcacg agctcgatcg
      241 agtagacctc cgccgcgaga agcgagagaa cggccgcctg gtatccggag ccggtgccga
      301 cttcgagaac gcgctcgcgg ccgtcgagct cgagggcctc gctcatgatg gcgacgattg
      361 tcggctgcga gatcgtctgc ccgtagccga tcggcgcagc cgtatcggca taggcccagc
      421 gcagcggcgt tcccggcatg aacgagtgac gggggacgcg ctgcatggca gccagcactc
      481 gcttgtcctg aatctcggac ccgatgcgac gaatgagagc ggcgcgcgcg tcccgcgaag
      541 tcatcggcgt cggtatgtgc ggtagcgata cccgtaattg acataccaat gacggtaggc
      601 cggccggtat atgcgccagt gatgcacgag cgctccgtag cccggatatt cgggcccgat
      661 ccacacgacc ccgccgttca catagtaaaa cggccgccct agctcgtcgt aataaacgac
      721 gtatccgtcg tagtacaagg gctcgtaccc atcgacgaag atgggtgccg tctcggcttc
      781 cacgtagcaa gccgacgccg gaccgacgac acaaaccaat agactggcga ccctccagag
      841 gcgattcatg caatgggttc gacgcaacgc ccgtgccgct cgcgcctcgt accggttttt
      901 cgtcgaactt gccgagaccc acgcacggct cgcgcgcgca cgcatccatt gcgcaaaccg
      961 cggcccgcca tggcgccgac gaaagctcac acttcctcgg ggaccatcgc gatagccccg
     1021 caggggcact cggcggcaca cagaccacaa cccttgcaga agtcgaggtc gacacggaac
     1081 cgcttgccgg ggcctagctt gatcacggca ttgtcggggc agactccata acagttgtcg
     1141 cattcgaagc agttgccaca tgacatgcat cggcgcgctt cgaagagtgc gttgtcggcc
     1201 gtgagaccgc cgtggatctc gtggaacgtc gcgcggcggc gcaaggcgtc gagcgtcggc
     1261 tgcaccgtac ggtccgcgtc g
//
LOCUS       DASUCJ010001168         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1051688, whole genome shotgun sequence.
ACCESSION   DASUCJ010001168 DASUCJ010000000
VERSION     DASUCJ010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051688"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..629
                     /locus_tag="VFU90_13735"
     CDS             153..629
                     /locus_tag="VFU90_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5480901.1"
                     /translation="MNAPRAVLLAAVSVALAACTPNGIGLAASGGGGGGGKTVTVDVN
                     LTLNQPVQTPYGLSGGMKPPITDVSVGDSVIFNNSDGFAHTSTSIPNATKFPAGSPFT
                     QGALKQSGTTLSGGWSSGAMQAGSSSQKVLVDKPGTYLYGCFFHYGAPMRGAIVAK"
     gene            619..>1660
                     /locus_tag="VFU90_13740"
     CDS             619..>1660
                     /locus_tag="VFU90_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480902.1"
                     /translation="MRNSVAVALAAAAFFFGLSSAPAHAIPIFAQRYRLHCGACHSVL
                     PELNGFGNYFRMHGYRLPIDKHGTTVFAIRYQMSYAQDPAAGTRRWTPGGIVLGNADI
                     GNISLYMHYSLGAGGGPSALYLGYLTNYNAHTHSEYRVGLFELPLAQSPGERLDDLQQ
                     YGYYGAHVGLNDLPLSSPRWGAWDERTIGNLRADLTVDVGEFQGAPYGGKPVDTGETS
                     FLAIPEVGVWLDQGIFAKGNNELDAGGEFLNGSRQILPSGRPSFIDPYQRYGLLAHTT
                     LGKIDLQAEQWWGNDHNADGFGTDQPSSGGYVRFKYYPIPHAYLAVRYDAQANPFVTR
                     DFTYYGAVMIAPF"
BASE COUNT          303 a          557 c          516 g          284 t
ORIGIN      
        1 cgcttgggca agagcggcta tcgcgaggag cacacgacct tcgtctacgt tttggatgca
       61 tccggtaagc tcagcaaaac gatgctggcg tcgaccggac tcaccggcga catcgtcgac
      121 gccgcgcgct cgctgcagcc gaggaccggc gaatgaacgc gccgcgcgcg gtgctgctgg
      181 cggcggtttc ggtcgcgctg gcggcatgta cgcccaatgg gatcggcctc gcggccagcg
      241 gcggcggcgg gggcggcgga aagaccgtta ccgtcgacgt gaacctcacc ttgaatcagc
      301 cggttcaaac gccgtacgga ctcagcggcg gtatgaagcc gccgatcacc gacgtcagcg
      361 tcggcgattc ggtcatcttc aacaatagcg acggattcgc acacacgtct acctcgattc
      421 cgaacgcgac gaaatttccg gccggttcgc cgttcaccca aggcgcgttg aaacaatcgg
      481 gcacgacgct ttccggcggg tggagcagcg gagcgatgca ggccggctcc agctcgcaga
      541 aagtcctggt cgacaagccc gggacgtacc tctacgggtg tttctttcac tacggtgcgc
      601 cgatgcgagg agcgatcgtt gcgaaatagc gtagccgtcg cgctcgcagc ggcggcgttt
      661 tttttcggac tctcgtccgc ccccgcgcac gcaattccga tctttgccca gcgctaccgg
      721 ctgcattgcg gggcctgtca cagcgtccta cccgaactca acgggttcgg caactacttc
      781 cgcatgcacg gttaccgctt gccgatcgac aagcacggaa ccaccgtgtt tgcgattcgc
      841 taccagatgt cgtacgcgca agatccggcc gcgggcacgc gccggtggac gccgggcggg
      901 atcgtgctgg gaaacgccga catcggaaac atctcgctgt acatgcacta cagcctggga
      961 gccggcggcg ggccgagcgc gctgtacttg ggctacctca cgaactacaa cgcacacacg
     1021 cactcggaat atcgcgtcgg actgttcgag ctgccgctcg cgcaatcgcc gggcgagcgg
     1081 ctcgacgacc tccagcagta cgggtactac ggcgcgcacg tcggtctcaa cgatttgccg
     1141 ctctcctcgc cgcgctgggg cgcgtgggac gagcgcacga tcggaaattt gcgcgcggat
     1201 ctcaccgtcg acgtcggcga gttccaaggc gcgccgtacg gcggtaagcc ggtcgacacc
     1261 ggcgagacgt cctttctggc gatccccgaa gtcggagtct ggctcgacca aggcatcttc
     1321 gccaagggca ataacgagct cgacgccggc ggcgagttcc tcaacggctc gcgtcaaatt
     1381 ctcccctcgg gccgcccgag cttcatcgat ccgtaccagc gctacgggct attggcgcac
     1441 acgacgctcg gcaagatcga cctccaggcc gaacagtggt gggggaacga ccacaacgcc
     1501 gacggcttcg gtaccgacca accctcgagc ggcggctacg tgcgcttcaa gtattatccg
     1561 atcccgcacg cgtatctcgc ggtccgctac gacgcacagg ccaacccgtt cgtaacccgc
     1621 gatttcacct actacggggc cgtcatgatc gcgccgttcc
//
LOCUS       DASUCJ010001169         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_73746, whole genome shotgun sequence.
ACCESSION   DASUCJ010001169 DASUCJ010000000
VERSION     DASUCJ010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73746"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VFU90_13745"
     CDS             <1..1452
                     /locus_tag="VFU90_13745"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5480903.1"
                     /translation="RPDSLAEFWPSVPYQAGMLDPATLSRLIARALRRGGDFADVFCE
                     RRSAQSFRLQDARIHESAFGVTVGVGIRVVSGESAGYAYSDDLSDEALERTADAASLI
                     ARSAVAGEARSLDLSLQPIPTFYGPGRNGHANSSQYVALLERADVAARAFDPRVIAVN
                     AHVTDELQEVWVATSEGRFVYDRRPLITLGVQAVATDKRERGSGYAGDGGRTSIAYFD
                     EHTPESLAQDAARIATVNVSAVPAPAGEMAMVVGAGGGGVLLHEAVGHGLESDFNRRG
                     TSLYSGRVGERVASELVTIFDDGNLPEERGSINVDDEGVPGQHKVLVENGVLRGYMQD
                     VLNARLMGVESTGSGRRQSFRFLPQPRMCNTYMPDGSSSVDEILASTPRGIYAKSFAG
                     GQVEISKGDFVFMVGEGYLIENGKITAPVKNATIVGNGPEAMTKVTAVGNDSRLARRH
                     YTCGKGGQYVPVGVGMPTVKIDSITVGGTQHDG"
     gene            1442..>1986
                     /locus_tag="VFU90_13750"
     CDS             1442..>1986
                     /locus_tag="VFU90_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU5480904.1"
                     /translation="MTDETQALEIAAAAAALAGTAGAAQAEATVSIARRFSTEVRERV
                     VSKLEQSTAKTLHLRVFVEGRKASLTSSDLSPDGLRAAVERTIEQAGHVAPDALAGLP
                     ERFAADVPELDLYDPGIELREGDAKVEEAMALERLIRRDDRRVVNSNGSHYSDAVSVT
                     AIANSAGFAAAYRSTRAGRSTS"
BASE COUNT          342 a          639 c          695 g          310 t
ORIGIN      
        1 cggccggatt cattagcgga gttttggccc tcggtgccct atcaagcggg catgctcgac
       61 cctgccacgc tctcgcgcct gatcgcccgc gcactgcgcc gcggcggcga cttcgccgac
      121 gtcttctgcg aacggcgcag cgcgcagtcg ttccggctgc aagacgcgcg cattcacgag
      181 agcgccttcg gcgtcaccgt gggggtcggc atccgcgtag tgtcgggcga gtcggccgga
      241 tacgcgtact ccgacgactt gagcgacgag gcgctcgaac gtaccgccga cgctgcgtcg
      301 ctgatcgccc gaagcgccgt cgcgggtgag gctcgcagcc tcgatttgag tctgcagccg
      361 attcccacgt tctacggccc gggccgcaac ggacatgcga actcctcgca gtacgtcgcc
      421 ttgctcgaac gcgccgacgt ggcggcgcgc gcgttcgatc cgcgcgtgat cgccgtcaac
      481 gcgcacgtga ccgacgagct gcaagaagtt tgggtcgcta ccagcgaggg gcgtttcgtc
      541 tacgaccggc gtccgctgat tacgctgggc gttcaggcgg ttgccaccga caagcgcgaa
      601 cgcggttcgg gctacgccgg ggacggcgga cgaacctcga tcgcatattt cgacgagcac
      661 acgccggaat cgctggcgca agacgccgcg cggatcgcga cggtgaacgt gtcggcggtg
      721 ccggcgccgg ccggcgaaat ggcgatggtc gtcggcgcag gcggcggcgg cgtgctgctg
      781 cacgaagccg tcggtcacgg cttggaaagc gacttcaacc ggcgcggtac gtcgctgtat
      841 agcggccggg tcggcgagcg cgtcgcgagc gagctcgtaa cgattttcga cgacggcaac
      901 ctccctgagg agcgcggcag catcaacgtc gacgacgaag gcgtccccgg tcagcacaaa
      961 gtgctggtcg aaaacggggt gctgcgcgga tacatgcagg acgttctcaa cgcgcggctg
     1021 atgggcgtcg agtcgaccgg aagcggccgc cgtcaatcgt tccggtttct gccgcagccg
     1081 cggatgtgta atacctacat gcccgacgga agctcgtcgg tcgacgagat cctggcgtcg
     1141 acgccgcgcg ggatctacgc caaatcgttt gccggcgggc aggtcgagat cagcaagggc
     1201 gatttcgtgt tcatggtggg cgaaggatac ctcatcgaaa acggcaagat cacggcgccg
     1261 gtcaagaacg cgaccatcgt cggcaacggt cccgaagcga tgaccaaagt aaccgcggtc
     1321 gggaacgaca gcaggctggc ccgccgtcac tatacgtgcg gaaagggcgg tcagtacgtg
     1381 cccgtcggcg tcggcatgcc gacggtgaag atcgactcga tcacggtcgg cggtacgcag
     1441 catgacggat gaaacgcagg cgctcgaaat cgcggcggcg gcggccgcgt tggccggcac
     1501 cgccggtgcg gcgcaagccg aagcgaccgt gtcgatcgcg cgccgcttca gcaccgaagt
     1561 tcgcgaacgc gtcgtgtcga agctcgaaca gtcgacggcg aaaacgctgc acttgcgggt
     1621 gttcgtcgaa ggccgcaagg cttcgctcac ctcgtcggat ttgtcgccgg acggtttgcg
     1681 cgcggcggtc gaacggacca tcgagcaggc cggacacgtc gcgcccgacg cgctcgcggg
     1741 gctgcccgag cgcttcgctg ccgacgtccc ggagctcgat ctctacgatc cgggaatcga
     1801 gctgcgcgag ggcgatgcaa aggtggaaga agcgatggcg ctcgagcgtc tgattcgccg
     1861 cgacgaccgg cgagtcgtca actcgaacgg ctcgcactac agcgacgccg tgtccgtgac
     1921 ggcgatcgcc aactctgccg ggtttgcggc ggcctatcgc tcgacgcggg ccggacgatc
     1981 gacctc
//
LOCUS       DASUCJ010001170         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1173966, whole genome shotgun sequence.
ACCESSION   DASUCJ010001170 DASUCJ010000000
VERSION     DASUCJ010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173966"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..>1579)
                     /locus_tag="VFU90_13755"
     CDS             complement(18..>1579)
                     /locus_tag="VFU90_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5480905.1"
                     /translation="TSALFGNFALAGIVNVHTLERMQGSEATVDAGTAGHIDATVLTG
                     FDHGPEGGGVFGARWDHEDGWRPNSRSDVEQGHFRVVHDLSPAWRIDGGIELYGAQWK
                     SPGFLSEDEFAARDYDVVSNPTDGGRKYRAQERVSLRLLSGPMLWRTTLYSTQGNWKF
                     FLTIPPAGGRFEGSGSQTEEVDHRTGLGATSALTWSLPHTDLTVGLESRWDESHYQNW
                     FSADRVADSAATLVHAKQTSGAMFVQSDIDATPRLRLSLGARYDLVDTHSTPEGELTA
                     GDSRGIVSPKLGALFRLTNALGVYANVSRGFRSTDGIIDDPSLPFVTAWAYESGLKLD
                     HDGVHGSVALFRMDVSNEQTFNPLSGASSNGGASRRQGVEVELEAPIAPMLSMSTDWT
                     FNDARYKRLITQAVDDPSVIDTLSGLRVYNTAQYVGIASLQFTPHAQPWRIRVSTNVV
                     GPYSPFDEPGVVLPAYGLLHVGGLIQIGVAQLEIGVRNVLDKAYPELVAGHVVSPGQP
                     RSFYGTVHYAF"
BASE COUNT          264 a          491 c          538 g          286 t
ORIGIN      
        1 caccacatct gtttccatta gaatgcatag tgcacagtcc cgtagaatga gcgcggttgg
       61 cccggggaaa cgacgtggcc ggcaacaagc tcggggtacg ccttgtcgag cacgtttcgc
      121 acgccgatct cgagctgtgc aacgccgatc tgaatgaggc cgccgacgtg cagcaggccg
      181 tacgcgggaa gcacgacgcc gggctcatcg aagggcgagt acggcccgac gacgttcgtg
      241 ctaacgcgaa tgcgccaggg ctgcgcgtgc ggcgtgaact gcagggaggc aatgcccacg
      301 tactgtgcgg tgttgtacac gcggaggccc gagagcgtat cgatgacgga cggatcgtcc
      361 actgcctgcg tgatcagtcg cttgtagcgg gcgtcgttga acgtccaatc ggtcgacatc
      421 gagagcatgg gcgcgatcgg agcctcgagc tcgacctcga cgccctggcg tcggctcgcg
      481 ccgccgttgc tcgacgcgcc gctgagcgga ttgaacgtct gctcgttgct gacatccatg
      541 cggaagagcg cgacggaccc atgcacgccg tcgtgatcga gcttgagtcc gctctcgtac
      601 gcccacgccg tgacgaacgg gagcgacggg tcatcgatga ttccatcggt cgagcggaat
      661 ccgcgcgaca cgttcgcgta cacgcccagc gcgttcgtta ggcggaagag cgcaccgagt
      721 ttcggcgaca cgatgccgcg gctgtcgccg gccgtgagtt cgccctccgg tgtggaatgt
      781 gtgtcgacga gatcatagcg cgcgccgagg ctgagccgca ggcgcggcgt cgcgtcgatg
      841 tccgactgga cgaacatcgc gcccgacgtc tgcttcgcgt ggaccagcgt ggcagcggag
      901 tccgccacgc ggtccgcgga gaaccagttc tggtagtgcg attcgtccca gcgcgattcc
      961 aagccgaccg tgaggtcggt gtgcggaaga ctccacgtga gcgcgctggt ggcgccgagt
     1021 cctgtgcggt gatcgacttc ttcggtttgg ctcccgctgc cctcgaagcg tccgcccgcc
     1081 ggcggaatgg tgagaaagaa tttccagttg ccttgcgtcg agtacagcgt ggtgcgccac
     1141 agcatggggc cggacaacag tctgagactg acgcgctctt gtgcgcgata ttttcgtcca
     1201 ccgtcggtgg ggttggacac gacgtcatag tcgcgcgcgg caaactcatc ttcgctgagg
     1261 aagccgggcg atttccattg tgcgccgtag agctcgatgc cgccgtcgat gcgccacgcg
     1321 ggcgagagat cgtgcacgac gcgaaagtgt ccctgttcga catcggaccg actgttaggc
     1381 cgccagccat cctcgtggtc ccagcgggcg ccgaatacgc cgccgccttc cgggccgtgg
     1441 tcgaatccgg tgaggacggt tgcatcgatg tgtcccgccg tgccggcatc gaccgtcgct
     1501 tcgctgccct gcatgcgttc gagcgtgtgg acgttcacga tgccggcgag cgcgaagttc
     1561 ccgaacagcg cgctcgttg
//
LOCUS       DASUCJ010001171         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_500902, whole genome shotgun sequence.
ACCESSION   DASUCJ010001171 DASUCJ010000000
VERSION     DASUCJ010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500902"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>1020
                     /locus_tag="VFU90_13760"
     CDS             53..>1020
                     /locus_tag="VFU90_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480906.1"
                     /translation="MTRRIRSVARAALYVGLLIFGTTGATAAGNPPAAAPAATAAAAP
                     ARVPSVEDFFRKPAYGRPELSPSGRYLAVIAPVGGHLGVGVIDLESKGVERMEAPEDS
                     DAIRVAWLNDKRIVITIGDLNRATGDPPTPRGYVVVDRDGSDQRILGGYNRVRIDSTV
                     RAGEFKRPWSVSVIRPIEGTSDLLVTALDRDIKSRDVYRYDTETGRAKLLSFESPGYV
                     TRWVVDFDNVPRAAVTDDIDGDKEAWYVRKSADSPWVKMEETHVGDLRSGPIQFDASG
                     KILYVWARRNGDDRASIYEYTVDTGAWKQVVSHPTRDIDDDTALFI"
BASE COUNT          184 a          323 c          355 g          158 t
ORIGIN      
        1 tgcggcgcgc gtccgcatgc ttgcggaacc ggaaagggag ggggacgctc cgatgacacg
       61 gcggatccga tcggttgcgc gcgcagcgct gtatgtcgga cttctgatct tcgggacgac
      121 aggcgcaacg gccgccggaa atccgccggc cgcggctcct gcggccaccg cggccgcagc
      181 acccgcgagg gtcccgagcg tcgaggattt cttccgcaag ccggcgtacg ggcggccgga
      241 gctgtcgccg agcgggaggt atctcgccgt catcgctccg gtcggcgggc atctcggcgt
      301 cggcgtgatc gacctcgagt cgaaaggcgt cgagaggatg gaggcgcctg aggactccga
      361 cgcgatccgc gtcgcgtggc tcaacgataa gcgcatcgtc atcacgatcg gtgacctcaa
      421 tcgcgcgacc ggcgatccgc ccaccccgcg cggatacgtc gtggtcgacc gggacggctc
      481 ggatcagaga atcctcggcg gctacaaccg cgtgcggatc gattcgacgg tccgtgcggg
      541 ggaattcaag cggccgtgga gcgtgtcggt gatccggccg atcgagggaa cgagcgatct
      601 cctcgtcacg gcgctcgatc gcgacatcaa gagccgcgac gtctatcgct acgacaccga
      661 gaccggccgt gcgaagctcc tttcgttcga gtctcccgga tacgtcaccc gctgggtcgt
      721 cgatttcgac aacgttccgc gggcggcggt caccgacgac atcgacggcg acaaggaagc
      781 ctggtacgtg cgtaagagcg ccgacagccc gtgggtgaag atggaggaga cgcacgtcgg
      841 cgatctcagg tccgggccga tccagttcga cgcatcggga aagattctct acgtgtgggc
      901 acggcgaaac ggtgacgacc gcgcttcgat ctacgaatac acggtcgaca ccggcgcatg
      961 gaagcaggtg gtgagccatc cgacccggga catcgacgac gacaccgcgt tgttcatcga
//
LOCUS       DASUCJ010001172         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_424869, whole genome shotgun sequence.
ACCESSION   DASUCJ010001172 DASUCJ010000000
VERSION     DASUCJ010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424869"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1761
                     /gene="carB"
                     /locus_tag="VFU90_13765"
     CDS             <1..>1761
                     /gene="carB"
                     /locus_tag="VFU90_13765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5480907.1"
                     /translation="DSLDTLRGALRQPTPERIFQVKRALAAGLTVAEVAEHSRIDPWF
                     LSQMMELIEAERDYAALPSVDASALRRMKRLGFSDRQLAALRGETEHAVRTQRWAAGV
                     RPVYKMVDTCAGEFPSATPYLYSSYDEESEAPPTGRRSVVILGSGPNRIGQGVEFDYC
                     CVRAVMALRERGFETIMINSNPETVSTDFDTSDKLYFEPLTFEDVLEIVDREQPVGVI
                     VQLGGQTPLKLTRALEAAGVAILGTSPDAIDIAEDRRRFEAIARATGVNQPESGTATS
                     LGEAVAIAARIGYPVLVRPSYVLGGRAMQIVYDESMLREYFDRAARASEDRPVLIDRF
                     LEDAYEVDVDAICDGERVVIGGVMQHIEDAGIHSGDSACVLPPYIIGESDLAAMREHT
                     VALAKALGVVGLINVQFAIKDRTVYVLEVNPRASRTIPFVSKAIGVPLASLAARVMLG
                     ESLSSLGFTDEVVPPYVSVKEAVFPFSKFREADPVLGPEMRSTGEVMGISDSFGSAFA
                     KAQLAADNGLPLEGSLLITVNNSDKANVIPIARRFQEMGFRLLATTGTAKFLRARGIP
                     AERVFKVHEGRPHGIDLIVNR"
BASE COUNT          293 a          575 c          601 g          292 t
ORIGIN      
        1 gactcgctgg acacgttgcg cggcgcgctg cgccagccga cgcccgagcg gatcttccag
       61 gtgaagcggg cgctcgccgc cggcctaacc gtggccgaag tcgccgagca ttcgcgcatc
      121 gacccgtggt tcctcagtca aatgatggag ctgatcgagg ccgagcgcga ctacgcggcg
      181 ctcccgtcgg tggatgcatc ggcgctgcga cgcatgaagc ggctcgggtt ctccgaccgg
      241 cagctcgccg cgctgcgcgg cgagaccgaa catgcagtgc gcacccaacg gtgggcggcg
      301 ggcgtgcggc ccgtctacaa aatggtcgac acctgcgccg gcgagtttcc gtccgccaca
      361 ccctacctct attcgagcta cgacgaagag agcgaagcac cgccgaccgg gcgccggtcg
      421 gtggtgattc tgggcagcgg gcccaaccgc attggccagg gcgtcgagtt cgactactgc
      481 tgcgtgcgcg cggtgatggc gctgcgcgag cgggggttcg agacgatcat gatcaactcg
      541 aatccggaga cggtgtcgac ggatttcgac acgtcggaca agctgtactt cgaaccgttg
      601 acgttcgagg acgtgctcga gatcgtggat cgcgagcagc cggtgggcgt gatcgtgcag
      661 ttaggcggcc agacgccgct caagctcacg cgggcgctcg aagcggccgg cgtcgcgatt
      721 ctcggcacgt cgccggatgc gatcgacatc gcggaagacc gccgtcggtt cgaggccatc
      781 gcgcgagcga cgggcgtgaa ccagccggag agcggcaccg ccacgagcct cggcgaagcc
      841 gtcgccatcg ccgcccgcat cggctatccg gtgctcgtcc ggccgtcgta cgtgttaggc
      901 ggacgcgcga tgcagatcgt gtacgatgag tcgatgctgc gcgaatactt cgaccgcgcc
      961 gcccgcgcgt ccgaagaccg gcccgtgctc atcgaccggt tcctcgagga tgcatacgaa
     1021 gtggacgtcg acgcgatctg cgacggcgag cgggtcgtga tcggcggcgt gatgcagcac
     1081 atcgaagatg cgggcatcca ctcgggcgac tcggcgtgcg tgctgccccc gtacatcatc
     1141 ggcgagtccg acctggccgc gatgcgcgag cacaccgttg cgttagccaa ggccctcggt
     1201 gtcgtcgggc tgatcaacgt gcagttcgcg atcaaggatc gcacggtgta cgtgctcgag
     1261 gtgaatccgc gcgccagtcg cacgattcca ttcgtgtcga aggccatcgg cgtcccgctc
     1321 gcctcgctcg ccgcgcgcgt gatgctcggc gagtcgctgt catcgctggg attcacggac
     1381 gaggtcgttc cgccgtacgt ctcggtgaag gaagcggtat tcccgttcag caaattccgc
     1441 gaggccgatc cggtgctcgg ccccgagatg cgatcgaccg gcgaagtgat gggcatctcc
     1501 gattcgttcg gcagcgcgtt tgccaaggcg cagctcgccg ccgacaacgg gctgccgctc
     1561 gagggctcgc tgctgatcac cgtgaacaat tccgacaaag cgaacgtcat tccgatcgcg
     1621 cgccgctttc aggaaatggg attccgtctg ctggccacca ccggcaccgc gaaatttctc
     1681 cgcgcgcgcg gcattccagc cgagcgcgtg ttcaaggtgc acgaagggcg gccgcacggg
     1741 atcgatctca tcgtcaaccg g
//
LOCUS       DASUCJ010001173         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1372589, whole genome shotgun sequence.
ACCESSION   DASUCJ010001173 DASUCJ010000000
VERSION     DASUCJ010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372589"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFU90_13770"
     CDS             complement(<1..731)
                     /locus_tag="VFU90_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480908.1"
                     /translation="MLVRGDPRGIAVVSTASDFASADYPRVVWSAVGIPEGVSVSLLW
                     STDLEPSRLNSVPIDIRAGRAIFVNVMDERHWIGHITGLALAFKGDLRSPIRIRGVTV
                     RAGGLVATLSGVARAWTSFAPWSMTSVDSLETHDTMSEVSPVAILFTSLAVTLVALVG
                     IAWLREKSLPAASFVSAASTHAPTLVVLTLFAWFVIDVGWAVGLARHVHDDNLRFAGK
                     TLTAKHLALEDGALYAFVEKARAILP"
BASE COUNT          196 a          330 c          333 g          154 t
ORIGIN      
        1 gggagaattg cgcgcgcctt ctcgacgaac gcgtagagcg cgccgtcttc gagcgcgagg
       61 tgcttcgccg tcagcgtctt gcccgcgaag cgcaggttgt cgtcatggac atggcgcgcg
      121 agtcctacgg cccacccgac gtcgataacg aaccaggcga acagcgtgag aacgaccagc
      181 gtcggcgcgt gcgtactcgc cgcacttacg aacgatgctg cggggaggct cttctcccgc
      241 agccacgcaa ttccgacgag cgccaccaac gtgacggcaa gcgaagtaaa gagaatggcg
      301 acgggcgaga cctcggacat cgtgtcgtgc gtttcgagcg agtcaaccga tgtcatgctc
      361 cacggagcaa agcttgtcca tgcgcgggcc acgccgctaa gggtcgcgac gagaccgccg
      421 gcccgtacgg tcactccccg gattcggatc ggcgatcgca gatcaccctt gaacgccagc
      481 gcgaggccgg tgatgtgtcc gatccagtgt cgctcgtcca tgacattcac aaagattgcg
      541 cgcccggccc ggatgtcgat ggggaccgag ttgagtcgcg atggctcgag gtccgtactc
      601 cagagcagcg atacgctgac cccttccggg atgccgacgg ctgaccagac gacgcgcgga
      661 tagtcagccg acgcgaaatc cgacgcagtc gagacgaccg cgatgccgcg cgggtcgccg
      721 cgaacgagca cgatctcgtc gcctgagcga atggcttcgc cccgcacaac ttccgttcgg
      781 gcggcgggga tgtcgatgtc ggacgcgcgt acggaccagg gtccgggcgt tttcgctagc
      841 gatccgagcg caacgcatgc gacaagagtg accgcgaatg ctaccgcgac ggtccgcagg
      901 cgccaccgca tcggacgcgg tcgttaactt acagcgcgtc ggccagccgc ggtgagcgcg
      961 acaagcagcg caccgatggc tgccccagcg gccgaatact tccaaatgcg gtt
//
LOCUS       DASUCJ010001174         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_364164, whole genome shotgun sequence.
ACCESSION   DASUCJ010001174 DASUCJ010000000
VERSION     DASUCJ010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364164"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VFU90_13775"
     CDS             complement(<1..1339)
                     /locus_tag="VFU90_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5480909.1"
                     /translation="MSATEVPSNGRHAIGLSMKRKEDARFIAGKGRYVDDIVLPNMLY
                     LAIVHSAYPHAKIVKIDKTAALAVPGVKAVITADDLAGVNLAWIPTFHGYDKQMVLAV
                     GKVLYQYQEVAAVIATSREAAADGVELVDVEYDPLEPVVSPFDAKTDRVLVREDRENK
                     TNHIYHWEVGKRAETDAALAASEVKIKERLYFQRCHPAPLEPCGCVADMNPATGTLTL
                     YMTSQAPHAHRTVVSLVTGIPEDKIHVISPDIGGGFGNKVPVYAGYVCSAVASIVTGA
                     PVKWIETRTENLTTTGFARDYHMDVEIGATKAGKVTALKVTTTADHGAFDAAADPSKY
                     PAGMFGICTGSYDFPVAFTELDAYFTNKAPGGIAYRCSFRVTEASYAIERGMDFLARA
                     LGQDPAEVRLKNFIRKEQFPYPSPLGFVYDSGDYHMTLNKALDAFGYADLRKEQ"
BASE COUNT          253 a          468 c          499 g          309 t
ORIGIN      
        1 cttgctcctt gcgcagatcg gcgtagccga acgcgtcgag cgccttgttc agcgtcatgt
       61 gatagtcgcc gctgtcgtac acgaatccga gcggcgacgg atacggaaac tgttccttac
      121 ggatgaagtt cttcaggcgt acttcggccg gatcttggcc gagcgcgcgc gcgagaaagt
      181 ccattccgcg ctcgatggcg taggacgctt cggtgacccg gaacgagcag cgataggcga
      241 tgccgcccgg agccttgttc gtgaagtacg cgtccagctc ggtgaaggcg actggaaagt
      301 cgtacgaccc cgtgcagatg ccgaacattc cggccggata cttactgggg tcggcagccg
      361 cgtcgaacgc accgtgatcg gccgtcgtcg tgaccttgag agccgtcact ttcccagctt
      421 tcgtcgcacc gatttcgacg tccatgtgat agtcgcgcgc gaatcccgtg gtcgtgaggt
      481 tttcggtgcg cgtttcgatc cacttcaccg gggcgccggt gacgatcgag gcgaccgcgc
      541 tgcacacgta gcccgcatag accggaacct tgttgccgaa accgccgccg atgtcgggtg
      601 aaatcacgtg aatcttgtct tcggggatcc cggtcaccag cgagacgacg gtgcgatgcg
      661 cgtggggcgc ctgcgaggtc atatacagcg tcagcgtacc ggtcgccgga ttcatgtcgg
      721 ccacgcatcc gcagggttcg agcggcgccg gatgacagcg ctggaagtag aggcgctcct
      781 tgatcttgac ctccgaagcg gccagcgcgg catcggtttc ggcccgcttg ccgacttccc
      841 aatggtagat gtgattggtc ttgttctcgc gatcctcacg gaccagcacc cggtcggttt
      901 tggcgtcgaa cgggctcacg accggctcga gcggatcgta ctccacgtcg accagctcga
      961 cgccgtcggc ggcggcctcg cgggacgtgg cgattacggc cgcgacctcc tgatactgat
     1021 acagcacctt cccgaccgcc agcaccatct gcttgtcgta gccgtggaag gtggggatcc
     1081 acgccaagtt cacgccggcg agatcgtcgg cggtgattac ggctttgacg ccgggaaccg
     1141 cgagagcggc ggtcttgtcg atcttgacga tcttggcgtg cgggtacgcg ctgtgaacga
     1201 tcgccagata gagcatgttg gggagcacga tgtcgtcgac gtaccggccc ttgccggcga
     1261 tgaagcgcgc gtcttctttg cgcttcatcg aaaggccgat cgcgtgccgg ccgttgctgg
     1321 gaacctcggt agcgctcacg tcatccgctc cttaggaagc tgcggcgggc tcggcggacg
     1381 acgccttggc cgcctgctgg accgctttaa cgatgttgtt gtatcccgtg cagcggcaca
     1441 tattgccgga gagcgcgtcg cgaatctcgg cttcggtggg atccggattg cctttgagaa
     1501 atgcgatcgc cgtcgccatc atgcccggc
//
LOCUS       DASUCJ010001175         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1357682, whole genome shotgun sequence.
ACCESSION   DASUCJ010001175 DASUCJ010000000
VERSION     DASUCJ010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357682"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="VFU90_13780"
     CDS             <1..>1094
                     /locus_tag="VFU90_13780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5480910.1"
                     /translation="NDWLIVNADIVNEGRRFNGDVRIRNGRIDAVAPSLAGHAGERTF
                     DAAGRLLIPGMIDDQVHMREPGLEHKGDFYTESRAAVAGGVTSFMEMPNSKPTTTDYP
                     ALADKYDRASKKSAANYAFYFGATNDNLDLIKNLDPRGVSGVKVFMGASTGNMLVDKP
                     EILDGIFRDCPVPVITHCEDTPTIEANLAIERAKYGDNIPVERHPYIRSREACVKSTK
                     LAIELAKRHGTRLHVLHISTADELELFMPGPTENKHITAETCVHFLHFSEEDYASKGN
                     LIKCNPAVKSAADRAAITQALKTGRIDVLATHHAPHTMEEKSQPYEKAPSGLPLVQYV
                     IQCALERWYDGLLPLELIVQKITHAPANLF"
BASE COUNT          237 a          374 c          321 g          162 t
ORIGIN      
        1 gaatgactgg ctcatcgtca acgccgacat cgtcaacgaa gggcgccgct tcaacggcga
       61 cgtgcgtatc cgcaacggcc gcatcgacgc ggtcgcgccc tcgctggccg gccacgccgg
      121 cgagcgcacc ttcgacgcgg ccggacgcct gctcattccc ggcatgatcg acgatcaagt
      181 ccacatgcgc gaaccgggac tcgaacacaa gggcgatttc tacaccgaat cgcgcgcggc
      241 ggtggcgggc ggcgtgacca gcttcatgga gatgcccaac tccaaaccga ccacgacgga
      301 ctatccggcg ctcgccgaca agtacgatcg tgcctcgaag aaatcggcgg cgaactacgc
      361 gttctacttc ggcgcgacca acgacaacct cgacctcatc aagaacctcg atccgcgcgg
      421 ggtcagcggc gtgaaagtgt tcatgggcgc ctcgacgggc aacatgctgg tcgacaagcc
      481 cgaaatcctc gacggcatct tccgcgactg cccggtgccg gtgatcacgc actgcgaaga
      541 cacgccgacc atcgaggcca atctcgccat cgagcgcgcg aagtacggcg acaacattcc
      601 ggtggagcgg cacccgtaca tacgttcgcg cgaggcgtgc gtgaaatcga ccaagctcgc
      661 catcgaactg gcaaagcgcc acggcacgcg tttgcacgtg ctgcacatct cgaccgccga
      721 cgaactcgag ctgttcatgc ccggcccgac cgagaacaag cacatcactg cggaaacctg
      781 cgtgcacttc ctgcatttca gcgaagagga ctacgcgagc aaaggcaacc tcatcaagtg
      841 caatccggcg gtgaagtcgg ccgcggatcg cgccgcgatc acccaagcgc tgaagaccgg
      901 ccgcatcgac gtgctcgcca cccaccacgc gccgcatacg atggaagaaa aatcgcagcc
      961 ttacgagaag gcgccgtcgg gtttgccgct cgtgcagtac gtgatccagt gcgcgctcga
     1021 acgctggtac gacggtttgc tgccgctgga gctcatcgtg cagaagatca cccacgcgcc
     1081 ggcgaacctg ttca
//
LOCUS       DASUCJ010001176         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1494998, whole genome shotgun sequence.
ACCESSION   DASUCJ010001176 DASUCJ010000000
VERSION     DASUCJ010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494998"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFU90_13785"
     CDS             <1..960
                     /locus_tag="VFU90_13785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5480911.1"
                     /translation="LRSGYVAQSVVDNDVTAVVAAHQAVIAARTAAKTARLGSSASQF
                     QAGSSTAQAAGAAASQRASEAAVRAAEAAVRQAQVNLAHATITSPVDGTVIQRNVSVG
                     QTVAAALQAPTLFTIAKDLRKMELDIAVGEPDVGAVRPGQRVFFSVLAYPGRIFSTTV
                     SEVRQNPTIVNNVTTYNTVAYPSTRDGALRPGMTATAKIVAATYPNALIVPLAALQWR
                     PSATAAKRYRVIAPPQPDRPLRAAASVWGTTGSAADFAVSVGSQGHCYVRDGNTLRGV
                     RLNVLAIEGTRVGVNVAAGSLSENDAVVTDEGNVANAVATSLP"
     gene            957..1673
                     /locus_tag="VFU90_13790"
     CDS             957..1673
                     /locus_tag="VFU90_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480912.1"
                     /translation="MTATVQTQDVSRLFPTGGTLFAAIDRITFELQPGEFTAIMGPSG
                     SGKSTFMNVIGCLDRATSGTYLLDGIDVSTLNANELARIRRTKLGFVFQQYNLLPRTS
                     ALENAELPMVYAGISARERHARAHEALLEVGLDERHQNSSPSELSGGQQQRVAIARAL
                     VNRPSLILADEPTGALDSKTSHELMELFLRLNRDLGITILMVTHELDIARYSRRIVSF
                     LDGRITSDEPVVRPQMAPVP"
BASE COUNT          304 a          593 c          573 g          284 t
ORIGIN      
        1 ctgcgctccg ggtacgtcgc gcagagcgtc gtcgacaacg acgtgaccgc ggtggtcgcc
       61 gcgcatcagg ccgtgatcgc cgctcggacg gcggcgaaga cggcgcgatt gggcagcagc
      121 gcgagccaat ttcaggccgg aagctcgacc gcgcaggctg ccggcgcggc tgcgtcgcag
      181 cgcgccagcg aagcggcggt acgcgcggcc gaagccgccg tacgccaagc gcaggtcaat
      241 ctcgctcacg ccaccatcac ctcgccggtg gacggaacgg taatccagcg caacgtctcg
      301 gtcggccaaa cggtcgccgc ggcactgcag gcgccgacgc tgttcacgat tgccaaggat
      361 ctgcgcaaga tggaactcga catcgcggtc ggcgagccgg acgtcggcgc ggtgcgcccc
      421 gggcagcgcg tctttttctc ggtgctggcc tatcccggac gaatcttttc gacgacggtt
      481 tcggaagtgc gtcagaatcc gaccatcgtc aacaacgtca ccacctacaa tacggtcgcg
      541 tatcccagca cccgcgacgg agcgctgcgt cccggcatga cggccaccgc gaaaatcgtt
      601 gcggcgacct atccgaacgc gctgatcgta ccgctggccg cactgcagtg gcgtcccagc
      661 gccaccgccg ccaagcgtta tcgcgtcatc gcgccgccgc aaccggaccg gccgctgcga
      721 gccgccgcct cggtgtgggg aaccaccggc agcgccgccg atttcgcggt ctcggtcggc
      781 agccaggggc attgctacgt gcgcgacggg aatacgctgc gcggcgtgcg gctcaacgtg
      841 ctggcgatcg aaggaacgcg cgtgggggtc aacgtcgcag ccggatcgct gagcgaaaac
      901 gatgcggtcg tgaccgacga aggcaacgtc gccaacgcgg tcgccacgag cctcccgtga
      961 cggcaaccgt tcaaacccag gatgtctcgc ggctgtttcc gaccggcgga acgctgttcg
     1021 ccgcgatcga tcgcatcacg ttcgagctgc agccgggcga gtttacggcc atcatgggcc
     1081 cgtcggggtc gggcaagtcc acttttatga acgtgatcgg ctgcctcgac cgcgcgacct
     1141 cggggacgta tttgctcgac ggcatcgacg tttctacgct gaacgccaac gaactggcgc
     1201 gcattcgccg gacgaaactc ggcttcgtct tccagcagta caatctgctg ccgcggacct
     1261 cggcgctcga aaacgccgag ctgccgatgg tgtacgccgg catttccgcg cgcgagcggc
     1321 acgcgcgggc gcacgaggcg ctgctcgaag tcggtctcga cgaacgtcat caaaattcct
     1381 cgccgagcga gctttccggc ggacagcagc aacgggtagc gatcgcgcga gcgctggtca
     1441 atcgtcccag cttgattctc gccgacgagc cgaccggtgc gctcgattcg aagacgtcgc
     1501 acgagctgat ggagctgttt ctcaggttga accgcgatct cggcatcacg atcctgatgg
     1561 tcacccacga gctggatatc gcgcgttatt cgcgccggat cgtcagcttt ctcgacggcc
     1621 ggatcacgag cgacgagccg gtcgtgcggc cgcaaatggc gccggttcca tgaacctgga
     1681 ggtcgcgatc gcaatcgctt tccgggcgct gatgcgcaac cgcgagcgct ctctgctgac
     1741 cgtgctcggc atca
//
LOCUS       DASUCJ010001177         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_791734, whole genome shotgun sequence.
ACCESSION   DASUCJ010001177 DASUCJ010000000
VERSION     DASUCJ010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791734"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="VFU90_13795"
     CDS             complement(<1..>1075)
                     /locus_tag="VFU90_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480913.1"
                     /translation="LGDVFAESADRGVYVTFDAGKTWTKTLYAGPRSGASDVAIDVRD
                     PRIVFAGIWEFRRQPWTFSSGGPDDGLYRSSDGGKTWKRLTGRGLPAGMTGRIGLAIA
                     PSDGRRIYALIEAKGGILWRSDDGGGSWKMVSNDTLVDQRPFYFTHIAVDPRNPNHVY
                     AVSEALSESKDGGKKFEEIAKDVHVDYHAIWIAPNDPARIIVGEDGGYALTVNGGKAW
                     SFSRNLAIGQFYHVAASNENPYYVCGGLQDNNAFCGPSNSLDPAGIKDDHWIDVVGGD
                     GMWAVPDPIDPTYIWTDLQDGRISLFDRKSQANRFVIPYFDFARNDFNIADRKYRFNW
                     DSPVAFAPWNGHIAWYGGNVVFQT"
BASE COUNT          203 a          341 c          330 g          201 t
ORIGIN      
        1 cgtctggaac acgacgttgc cgccgtacca tgcaatatgg ccgttccagg gcgcaaacgc
       61 gaccggagaa tcccagttga agcgatactt ccggtcggcg atattgaaat cgttgcgcgc
      121 gaaatcgaaa tacggaatga cgaaccggtt ggcttgcgac ttgcggtcga agagcgatat
      181 ccgcccgtcc tgcagatcgg tccatatgta ggtgggatcg atcggatcgg gcacggccca
      241 catgccgtcg ccgccgacga cgtcgatcca gtggtcgtcc ttaatgccgg ccggatcgag
      301 cgagttcgag ggtccgcaga aggcgttgtt atcctgcaga cctccgcaca cgtaatacgg
      361 attttcgttc gaggcggcca cgtgatagaa ctgaccgatc gcgaggttgc gcgagaacga
      421 ccatgccttt ccgccgttga cggtgagcgc gtacccgccg tcctcgccga cgatgatgcg
      481 cgccggatcg ttgggagcga tccaaattgc gtggtagtcg acgtgcacgt ctttggcgat
      541 ctcttcgaac ttcttcccgc cgtccttact ctccgagagc gcttccgata ccgcgtacac
      601 gtgattggga ttgcgcggat cgaccgctat gtgcgtgaaa tagaacgggc gctgatcgac
      661 cagcgtgtcg ttggagacca tcttccacga gccgccgccg tcgtccgaac gccagaggat
      721 cccgccctta gcttcgatca gcgcgtaaat gcggcgcccg tcgctcggcg cgatcgccaa
      781 gccgatgcgg cccgtcatgc ccgccggaag cccgcgaccg gtcaaccgct tccaggtttt
      841 tccaccgtcg gacgaacggt acaagccgtc gtcggggccg ccgctcgaaa acgtccacgg
      901 ctgccggcgg aattcccaaa tacccgcgaa gacgatgcgg ggatcgcgca cgtcgatcgc
      961 aacgtcggac gcaccgctgc gcggaccggc gtagagcgtc ttcgtccacg tttttcccgc
     1021 gtcgaacgtt acgtagacgc cgcgatcggc ggactcggcg aagacgtcgc caagc
//
LOCUS       DASUCJ010001178         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_546990, whole genome shotgun sequence.
ACCESSION   DASUCJ010001178 DASUCJ010000000
VERSION     DASUCJ010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546990"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFU90_13800"
     CDS             complement(<1..577)
                     /locus_tag="VFU90_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480914.1"
                     /translation="MTNAGVSHTGSRTRQVVRVYYAIGMMSVGVLALVYGFGVLVAEK
                     PPAWVPSPNGIAYAAGVLMLATGAGLLFERSSRVSIRILLPFMLLWTLTRVPVPIMEP
                     GREIAWFLVGEIAVPAAGALVLFAWYADLRPGSTLDALTRQYAIPCARILIGLSVPTF
                     GLSHFFEFAARTVTLVPPWLPYPKFWADAMGA"
     gene            complement(582..>1180)
                     /locus_tag="VFU90_13805"
     CDS             complement(582..>1180)
                     /locus_tag="VFU90_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5480915.1"
                     /translation="EGPRPEMFVPYTQDPYPTFSTMQFVVRSSGDPTRLVAAIRRAVA
                     SVDPDIPLARIRTIDELVSDASANARFATLVMSSFGVMALALAMIGLYGVIAFMVHQR
                     RQEFGLRTALGATREQLVQMVLVDGFMLACVGMVAGVVLALVAGRLLRSMLYQVSVAD
                     PLTLVAVVLLLLGTTALACVLPAVRASGVDPRAALDAG"
BASE COUNT          239 a          421 c          379 g          141 t
ORIGIN      
        1 cggcgcccat cgcgtccgcc cagaacttcg ggtacggcag ccagggcggc accagcgtca
       61 cggtgcgcgc agcgaactcg aagaaatgcg agagcccgaa cgtcggcacc gagaggccga
      121 tgagaattcg ggcacacgga atcgcgtatt gccgcgtgag cgcgtccaga gtcgagcccg
      181 gcctcaaatc cgcataccat gcaaagagca ccagcgcccc agccgccggc accgcgatct
      241 cgccgacgag aaaccaggcg atttcccggc ccggctccat gatcggaacc ggcacgcgcg
      301 tcagcgtcca cagcagcatg aacggcagga gaatgcgaat cgaaacgcgc gacgagcgct
      361 cgaacagaag accggcgccg gtggccagca tgagcacgcc cgcggcgtag gcgataccgt
      421 tgggcgacgg cacccacgcc ggcggcttct cggcgacgag cacgccgaag ccatagacga
      481 gcgcgaggac gcccacgctc atcatgccga tcgcgtaata gacccggacg acctgccggg
      541 ttcggctgcc cgtatgcgac acgcctgcgt tggtcatgtt gtcaaccggc gtcgagcgcc
      601 gcgcgagggt ccactccgga tgcacgcacc gccggcagca cgcacgccaa cgcggtcgtg
      661 cctaacagta gcagcaccac ggcgaccagc gtcagggggt cggcaacgct cacctgatag
      721 agcatcgatc gcagcagccg ccccgcgacc agcgccagca cgacgcccgc caccatcccg
      781 acgcacgcga gcatgaaccc gtcgacgagc accatctgga cgagctgttc gcgcgtggcg
      841 ccgagcgcgg tgcgcagacc gaattcctgg cggcgttggt gcaccatgaa tgcgatgaca
      901 ccatacaggc cgatcatggc cagcgcgagg gccatcacgc caaacgagct catgaccaac
      961 gtcgcgaacc gcgcattcgc gctggcgtcg ctcaccagct cgtcgatggt gcggatgcgc
     1021 gcgagcggaa tgtcgggatc gaccgacgcc accgcccggc ggatcgccgc caccagccgc
     1081 gtcgggtcac ccgagctccg cacgacgaac tgcatcgtcg agaacgtcgg atacgggtcc
     1141 tgcgtgtacg gcacgaacat ctcgggtctc gggccttccg
//
LOCUS       DASUCJ010001179         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_165295, whole genome shotgun sequence.
ACCESSION   DASUCJ010001179 DASUCJ010000000
VERSION     DASUCJ010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165295"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /gene="asnB"
                     /locus_tag="VFU90_13810"
     CDS             complement(<1..1156)
                     /gene="asnB"
                     /locus_tag="VFU90_13810"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5480916.1"
                     /translation="MLARDHFGKKPLFIQESGGAVLFGSEIKAILSFGGATPSLNRAS
                     LADYFVYRYVPAPHTLFAGIRKLMPGSYAVWEKGHLTETAFYHPPYGDAPEIPSISDP
                     VAAFTERLDDAVRVRMVSDVPFGAFLSGGLDSSAIVALMSRHAAQPINTFSVGFREAR
                     YSELPYAASIARQFGTNHTELTIAADDLMQHLPTLIDHQDAPVAEASNIPIYLISREA
                     AKSVKMVLTGEGSDELLGGYPKHAAERYAAIYQRLVPACAHDRVVAPLINVLPYRFRR
                     IKILAASIALRDPAERMARWMGALTYTERDKLLSGVQERRPIDPEPFRVDGRRSALER
                     VLYFDQTSWLPDNLLERGDRITMAASIEARMPFMDTELARFMARLPDKWRI"
BASE COUNT          228 a          381 c          396 g          194 t
ORIGIN      
        1 gaatacgcca tttgtcgggc aggcgcgcca taaaccgcgc cagttccgta tccatgaacg
       61 gcatgcgcgc ttcgatcgac gcggccatcg tgatgcggtc gccgcgctcg agcagattgt
      121 cgggcagcca cgacgtctgg tcgaaataga gcacgcgttc gagagcggaa cgccgcccat
      181 cgacccggaa cggttccggg tcgatgggtc gccgctcttg cacccccgac agaagcttgt
      241 cgcgctcagt ataggtcaga gcgcccatcc agcgcgccat gcgttcggcc gggtcgcgca
      301 gcgcgatcga cgccgcgaga atcttgatcc ggcggaaccg gtacggcaac acgttgatca
      361 gcggcgccac gacgcgatcg tgcgcgcaag ccggcacgag acgctgatag atcgcggcat
      421 agcgttccgc ggcgtgtttg ggatagccgc cgagcagctc gtccgagccc tcgcccgtca
      481 gcaccatctt cacgctcttg gccgcctcgc gagagatcag atagatcggg atgttcgacg
      541 cctccgccac cggcgcgtct tgatggtcga tcagcgtcgg cagatgctgc atcagatcgt
      601 cggcggcgat ggtcaactcg gtatggttgg tgccgaactg gcgcgcgatg ctcgcggcgt
      661 aaggcagttc cgaataacgc gcctcgcgga aaccgaccga gaacgtgttg atcggctgcg
      721 ccgcgtggcg gctcatcagc gcgacgatgg cggatgaatc gagcccaccc gataggaacg
      781 ccccgaacgg cacgtccgat accatgcgaa cgcgcaccgc gtcgtcgagc cgttcggtga
      841 acgcggcgac cggatcggaa atcgagggga tttcgggcgc gtcgccatac ggcggatggt
      901 agaaggccgt ctcggtcaga tgccctttct cccacacggc gtaggagccg ggcatgagct
      961 tgcggatgcc ggcgaacagc gtatgcggcg ccggcacata gcgatagacg aaatagtcgg
     1021 cgagcgaggc gcggttgagc gacggcgtcg cgccgccaaa cgacaggatc gccttgatct
     1081 cggagccgaa cagaaccgcg ccgccgcttt cctgaatgaa tagcggcttc ttgccgaaat
     1141 gatctcgcgc cagcatgaga cgctggttcc cgtcgtccca cagcgcgaac gcgaacatg
//
LOCUS       DASUCJ010001180         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_975540, whole genome shotgun sequence.
ACCESSION   DASUCJ010001180 DASUCJ010000000
VERSION     DASUCJ010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975540"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFU90_13815"
     CDS             complement(<1..264)
                     /locus_tag="VFU90_13815"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEU5480917.1"
                     /translation="MPKRTRIYTRTGDDGTTGLIGGRRIKKSAPRIETYGTVDELSSA
                     IGVVRAGLDTGGERAARLDAWLAWTQDRLFNLGSSLAAMPGERP"
     gene            358..>1031
                     /locus_tag="VFU90_13820"
     CDS             358..>1031
                     /locus_tag="VFU90_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU5480918.1"
                     /translation="MSEALPQAALREIPPKAADLSAWYTAVCLKAELVSYSPVRGCVV
                     LRPYGFGMWENLQKHLDARFKATGHENAYFPLFIPEHLLTREAEHVEGFAPEVAWVTH
                     GGQEELTERLAVRPTSEAIIGTMYAQWIESYRDLPLLINQWANVVRWEKATRPFLRTM
                     EFLWQEGHTAHATAAEAREETLRMLDVYRDFAENVAGVPVYAGVKSAGERFPGAVETY
                     SIEALMP"
BASE COUNT          171 a          335 c          346 g          179 t
ORIGIN      
        1 gggccgctcg ccgggcatcg ccgccaacga ggagccgagg ttgaataaac ggtcttgagt
       61 ccaggctagc cacgcatcga gccgtgcggc ccgctcgccg ccggtatcca aaccggcgcg
      121 cacgacgccg atcgcgctcg acagctcgtc gaccgttccg taagtctcga tccgcggcgc
      181 actcttcttg atgcgccgtc cgccgatcag tccggtcgtg ccgtcgtcgc cggttcgagt
      241 gtagatgcgc gtgcgtttcg gcatgccgcg aacgtttcgc cgcaccggcg ccgatatccc
      301 gaacgcagcg ccggcgcagg aggcggcgag cggcgggcgc acgaagcgtg gccggtcatg
      361 tccgaggccc taccccaggc cgccctgcgg gagatacctc ctaaggcggc cgatctttcc
      421 gcgtggtaca cggcagtctg cctgaaggcc gaactggtct cgtactcgcc ggtccgcgga
      481 tgcgtcgttt tgcggccgta cggcttcggg atgtgggaga acctgcagaa gcacctcgac
      541 gcccgtttca aggcgactgg gcacgaaaac gcttactttc cgctctttat tcccgagcat
      601 ttgctcaccc gcgaggccga gcacgtcgaa ggcttcgcgc ccgaggtagc gtgggtgacg
      661 cacggcggtc aggaagagct caccgagcgg ctggccgtgc gtccgacctc cgaggcgatc
      721 atcgggacga tgtatgcgca gtggatcgaa tcctatcgcg acctgccgct gctgatcaat
      781 caatgggcga acgtggtgcg ctgggagaag gcgacgcgcc cgttcctgcg tacgatggag
      841 tttctgtggc aagagggaca caccgcgcat gcgaccgcag ccgaggcgcg cgaggaaacc
      901 ttgcgtatgc tcgacgtcta tcgcgatttc gccgagaacg tcgcgggcgt cccggtctat
      961 gccggcgtga agagtgccgg cgaacgcttt ccgggagccg tcgagacgta ttcgatcgaa
     1021 gcgctcatgc c
//
LOCUS       DASUCJ010001181         1271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_364272, whole genome shotgun sequence.
ACCESSION   DASUCJ010001181 DASUCJ010000000
VERSION     DASUCJ010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364272"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFU90_13825"
     CDS             <1..447
                     /locus_tag="VFU90_13825"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEU5480919.1"
                     /translation="RCVAGRARAWYLGDRHYPQGWERSAHDFLSPGLAEADLMRRVLD
                     RDEFGAWWAAFLGAAPATAAIFTVAGVPDVADGQLVHLHGLNLSRAAMLARISQSLAG
                     GALDLPARAERLYRAGAGVAVEGDYLSTHWLATFAWDAATSLDAIG"
     gene            complement(444..>1271)
                     /locus_tag="VFU90_13830"
     CDS             complement(444..>1271)
                     /locus_tag="VFU90_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5480920.1"
                     /translation="VNDAVALHNADVGISVDSGTDVAKDAADIVLLNKDLGILADGVV
                     EGRRIFANTIKYVLMGTSSNFGNMFSAAGASLFLPFLPMTAPQILLNNLLYDVGEMTI
                     PTDEVDEELLQKPAHWDVGFIRRFMLVFGPISSVFDFLTFGVMLLVFHARPALFQSGW
                     FVESLLTQTLIIFAIRTRRIPFWKSSPSLPLTITSLAVVATGAVLPFTPIAAIFGFVP
                     LPAAFLAILLGMIVTYLCLVETAKWFFFRPRAGAPTPATVKHQLRLGRVAARFRGTR"
BASE COUNT          234 a          392 c          438 g          207 t
ORIGIN      
        1 cgctgcgtgg ccgggcgggc gcgagcgtgg tatctcggcg accgccacta cccgcaaggt
       61 tgggagcgca gcgcacacga ctttctttcg cccggattgg cggaagccga tctgatgcgg
      121 cgcgtgctcg accgcgacga attcggcgcg tggtgggcgg cgtttctcgg cgcggcgccg
      181 gctaccgccg caattttcac ggtggccggc gtaccggacg tcgccgacgg tcagctcgtc
      241 cacttgcacg gattgaatct gtcgcgcgct gcaatgctcg cgcgcatctc ccagtcgctc
      301 gcgggcggtg cgctcgacct gccggcgcgt gccgaacggc tctaccgggc cggggccggc
      361 gtcgccgttg agggcgacta cctgtcgacg cattggctgg ccacgtttgc ttgggacgcc
      421 gccaccagcc tcgacgcgat cggctaacgc gttccgcgaa agcgcgcggc gacgcgcccc
      481 aagcgcagct ggtgtttcac cgtcgccggc gtcggcgcgc cggcgcgcgg ccggaagaag
      541 aaccacttgg cggtctcgac gagacacaag tacgtaacga tcattccgag gagaatcgcg
      601 agaaaggccg ccggcagcgg cacgaagccg aagatcgccg cgatcggggt gaacggcagc
      661 acggcgccgg tggcgacgac ggccagcgag gtaatcgtga gcggtaagct cgggctgctt
      721 ttccagaacg gaatacggcg cgtgcgaatc gcgaagatga tcaacgtttg cgtcagcagc
      781 gattcgacga accagccgga ctgaaagagc gcgggacgag cgtggaagac cagcagcatc
      841 acgccgaacg ttaagaagtc gaacaccgag ctgatcgggc cgaagaccag catgaaacgc
      901 cggatgaagc cgacgtccca atgcgccggc ttttgcagca gctcttcgtc gacttcatcg
      961 gtcgggatgg tcatctcgcc cacgtcgtag agcagattgt tgaggagaat ctgcggcgcg
     1021 gtcatcggga gaaacggtaa gaacagcgac gcacccgcgg cgctgaacat gtttccgaag
     1081 ttcgacgagg tgcccatcag cacgtacttg atcgtgttgg caaagatgcg gcgcccttcg
     1141 accaccccgt cggcgagaat tccgaggtct ttgttcagga gtacgatgtc ggcggcgtcc
     1201 ttggcgacgt cggttccgga atcgaccgag atgccgacgt cggcgttgtg aagcgccacg
     1261 gcgtcgttga c
//
LOCUS       DASUCJ010001182         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1174108, whole genome shotgun sequence.
ACCESSION   DASUCJ010001182 DASUCJ010000000
VERSION     DASUCJ010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174108"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFU90_13835"
     CDS             complement(<1..363)
                     /locus_tag="VFU90_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5480921.1"
                     /translation="MPKTVLITGGSRGIGRVTAMRLLADGYNVAFTYTQSKAAAEEFV
                     GTDKARLHAYAGDVRDDARNREIVENVVARFGTIDALVNNAGIRRDALMFNMSGEQWD
                     EVVDTNLRGAFSMIHAVLP"
     gene            complement(369..1064)
                     /gene="purQ"
                     /locus_tag="VFU90_13840"
     CDS             complement(369..1064)
                     /gene="purQ"
                     /locus_tag="VFU90_13840"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5480922.1"
                     /translation="MKFGIVVLPGSNCDHDALHVTTDLLGAEAEVLWHKETDLKGVDC
                     VIVPGGFAYGDYLRAGALAKFAPIMEPIRRHAAAGGLVFGICNGFQVLTEVGLLPGAL
                     MRNAHLRFLGRDVLLRTEETDTPFTSELTRGQVLRMPIAHGEGNYYADDATLDELERN
                     RQVIFRYCDSEGRLTPDANPNGSARNIAGICNRTRNVFGMMPHPERCAEALLGNADGL
                     GVFKSIAASLARV"
BASE COUNT          186 a          379 c          385 g          209 t
ORIGIN      
        1 cggcagcacc gcatgaatca tggagaatgc gccgcgaaga ttcgtatcga ccacctcgtc
       61 ccactgctcc ccgctcatgt tgaacatcag cgcgtcgcgg cggatcccgg cgttgttgac
      121 cagggcgtcg atcgtgccga agcgagccac gacgttctcg acgatctcgc ggttgcgtgc
      181 gtcgtcgcgc acatcgccgg cgtacgcatg cagccgcgcc ttgtcggtgc cgacgaattc
      241 ctcggccgcc gctttcgact gcgtgtacgt gaacgccacg ttgtagccgt cggcgagaag
      301 gcgcatcgcg gtgacacggc cgatgccgcg cgatccgccg gtgatcagca ccgttttcgg
      361 cacgctgctc acactcgggc gagcgaagca gcgatcgact tgaagacgcc cagcccgtcc
      421 gcgttgccga ggagcgcctc agcgcatcgc tccggatggg gcatcatgcc gaacacgttg
      481 cgcgtgcggt tgcagatgcc ggcgatgttg cgcgccgagc cgttcggatt cgcgtccggc
      541 gtgagccggc cttcgctgtc gcagtagcgg aagatgacct gccggttgcg ctcgagctca
      601 tcgagcgtcg cgtcgtccgc gtagtagttg ccttcgccgt gcgcgatcgg cattcgcagc
      661 acctgccccc tggtcagctc gctcgtgaac ggcgtgtcgg tttcttcggt gcgaaggagc
      721 acatcgcgtc cgagaaagcg gaggtgggcg ttgcgcatga gcgcgccggg cagcaacccg
      781 acttcggtga gcacctggaa tccgttgcag atgccgaaca ccagcccgcc cgcggcggca
      841 tgacggcgga tcggctccat gatcggagcg aacttcgcca gcgcgccggc gcgaaggtaa
      901 tcgccgtacg cgaatccgcc ggggacgatc acgcagtcga cgcccttcag atccgtctcc
      961 ttgtgccaca acacctcggc ctcagcgccg aggagatcgg tggtgacgtg gagcgcgtcg
     1021 tgatcgcagt tggagccggg cagaacgacg atgccgaatt tcatctacgt ttttccggtg
     1081 gagcggcggg ctttagcccg tcgtcggcgg cctgaaggcc gccgctcaac gtttcaatgc
     1141 gcaatcgcgg gtctcggta
//
LOCUS       DASUCJ010001183         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_852849, whole genome shotgun sequence.
ACCESSION   DASUCJ010001183 DASUCJ010000000
VERSION     DASUCJ010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852849"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..572
                     /locus_tag="VFU90_13845"
     CDS             171..572
                     /locus_tag="VFU90_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5480923.1"
                     /translation="MTLGIDTNVLVRYLVRDDEAQFAQASNLLSRLSDADETAFVSLL
                     VVQETEWVLRSRYRIGKHEIIDTFSALLDTVDLIFEDEPALEESLVTWGDSDAEFSDC
                     LIGAHNRRAGCRATATFDQRAQALDSFTAVE"
     gene            573..>1179
                     /locus_tag="VFU90_13850"
     CDS             573..>1179
                     /locus_tag="VFU90_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU5480924.1"
                     /translation="MRFPMQPVNAALPRIDVKADFWSLRFVDETSEAYCVRKNVAQPL
                     ALATDRGVMASVYADGGYGYAATADTSPAGLRAALDRAAEWARASAKLAITDSRTLPR
                     PAARGQYESPALASPLPDARGWFELLHGLARDAGCDARIVDWDASVDVIHSTQRLVTS
                     EGGDVVQRFRMVIPSVGVTAHADGVTQQRSLNGQRGICQQGG"
BASE COUNT          202 a          394 c          377 g          206 t
ORIGIN      
        1 ccgaagggga ttcgtgacca actcggcatg cggcccggcg acaagatgac attcacggta
       61 ctcccgaacg gtacggttct gctgcgaatg aagcgcaaac atgtcttcga actcgccggg
      121 gtccttcacc gcaaggggcg ctcgcggatc gccgtcgagg acatgacgcg ttgaccctcg
      181 ggatcgatac caacgttctc gtccggtacc tggttcgaga cgacgaggct caattcgcgc
      241 aggcaagtaa tctgctctcg cgcttgtccg atgccgacga aacggcattt gtcagccttc
      301 tggtggtgca ggaaaccgaa tgggtgctgc gaagccgcta tcgaattggc aagcatgaga
      361 tcatcgacac attttccgcc ctgctcgata ccgtcgatct tattttcgag gacgagcccg
      421 cgctggaaga gtcgctggtc acctggggtg atagcgatgc cgagttctcc gattgcctca
      481 tcggcgctca caaccggcgc gccggatgcc gcgcgaccgc gacgttcgat caacgcgcac
      541 aggcgctcga ttccttcaca gccgtcgaat aggtgcgatt cccgatgcaa cccgtgaatg
      601 ccgcgctacc aaggatcgac gtcaaggccg acttctggtc gctgcgcttc gtcgacgaga
      661 cttccgaagc gtattgcgtg cgcaagaacg tcgcgcagcc actcgcgctc gcgaccgacc
      721 gcggcgtgat ggcgtcggtc tacgccgacg gtggctacgg ctacgccgcg acggccgaca
      781 cctcgcccgc cggacttcgc gcagcgctcg accgcgccgc cgaatgggcg cgtgcgagtg
      841 cgaagcttgc gatcaccgat tcgcgcacgc tgccgcggcc cgcggcgcgc gggcaatacg
      901 aatcgcccgc gctcgcttcg ccgctccccg atgcgcgcgg ctggttcgag ctcctccacg
      961 gtctcgcgag ggacgcgggt tgcgacgcgc ggatcgtcga ctgggacgcg tcggtcgacg
     1021 tcatccactc gacgcagcgc ctcgtgacga gcgaaggtgg cgacgtcgtc cagcgtttcc
     1081 ggatggtgat cccgtcggtc ggcgtcaccg cgcacgccga tggcgtcacc cagcagcgct
     1141 cgctcaacgg tcagcgcggc atctgccagc agggcggcg
//
LOCUS       DASUCJ010001184         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1143713, whole genome shotgun sequence.
ACCESSION   DASUCJ010001184 DASUCJ010000000
VERSION     DASUCJ010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143713"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1006
                     /locus_tag="VFU90_13855"
     CDS             30..>1006
                     /locus_tag="VFU90_13855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5480925.1"
                     /translation="MTARRTFLYIGALGALLSIASPSIVRAQQTPDLAAFDRYVAKAA
                     RDWRVPGLAIAIVKDDSLVFAKGYGVIELGKPAPVNEHTRFAIGSTTKAMTSAALAML
                     VDEGKLQWDERVIDILPELQLYDAYATHELTVRDLLTHRSGLPDTDLLWIIPQNNLSM
                     EEMIRRLRYVKPESSFRSHWDYQNVVYAIAGLIVEKLSGMPWKQFVQTRIWDPLGMHE
                     TLPLVSALAGQPNVAVPHALVNDTVRRVPIRTTDAIAPAGSVWSSVSDMSKWMRFVLD
                     SGRVGTKQLIKPSTFAELLAPQIRAPMEEYPALELARPHFFSYALGWFV"
BASE COUNT          180 a          327 c          312 g          187 t
ORIGIN      
        1 gtggcgacca cgcgcaccgg cggcaaccaa tgaccgctcg gcggacattc ctttacatcg
       61 gtgcactcgg cgcgctgctt tccatcgcgt cgccgtccat cgttcgtgcg cagcaaaccc
      121 ccgatctggc cgcgttcgat cgctacgtgg ccaaggccgc gcgcgactgg cgggtgcccg
      181 gtctcgcgat cgcgatcgtg aaagacgatt cgctcgtgtt cgccaagggc tacggcgtga
      241 tcgagttagg caagcccgct ccggtgaacg aacacacgcg attcgccatc ggctcgacca
      301 cgaaagcgat gacgtccgcg gcgttggcga tgctcgtgga cgagggcaag ctgcaatggg
      361 atgaacgcgt catcgacatc ctgcccgagc tccaactgta cgacgcgtac gcgacacacg
      421 agcttaccgt ccgcgatttg ctgacgcacc gcagcggact gcccgatacc gacctgttgt
      481 ggatcattcc gcagaacaat ctcagcatgg aagagatgat tcgccgtctt cgctacgtga
      541 agccggagtc gtccttccga tcgcattggg actatcagaa cgtcgtctac gcgattgccg
      601 gcctgatcgt cgagaagctt tccgggatgc cgtggaaaca attcgttcag acgcgcatct
      661 gggacccgct cggcatgcac gagacgcttc cgctggtatc ggcattagcg gggcagccta
      721 acgtagccgt gccgcacgcg ctcgtgaacg acaccgtgcg ccgggtgccg atccgcacca
      781 ccgatgcgat cgcgccggcc ggctccgtgt ggtcgagcgt gtccgacatg tccaagtgga
      841 tgcggttcgt cctggacagc ggccgagtgg ggacgaagca gctcatcaaa ccatccacgt
      901 tcgccgagct gctcgcgccg cagattcgcg cgccgatgga ggagtatccg gcgctcgagc
      961 tggcccgtcc gcatttcttc agttacgcgc tcggctggtt cgtgca
//
LOCUS       DASUCJ010001185         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_104520, whole genome shotgun sequence.
ACCESSION   DASUCJ010001185 DASUCJ010000000
VERSION     DASUCJ010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104520"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFU90_13860"
     CDS             <1..909
                     /locus_tag="VFU90_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEU5480926.1"
                     /translation="GDAWLFCDGPVHPSGREELVFGQRGVTGFELTIYGPTRPLHSGH
                     YGNWAPNPGILLASLIASMRDPDGHIKIAGYYDNVRPITAAEHKAIAALPEYDATLRR
                     SLGLARTEANDAPLAERIMLPALNLRGMRVGGVRETGSNTIPSVAYASVDLRLVPNET
                     PARVQHLVEEHIRRQGFYLTHDSATTAERLAHPKIARIEWESGYPASREPMDGPFGRA
                     VLATLNDGAPAPLLALPTMGGSGPTYLFEQILHVPMITLPIANYDDNQHAADENLRLQ
                     NLWNGIELYATLLSGIGRHWGAATMP"
BASE COUNT          186 a          361 c          327 g          158 t
ORIGIN      
        1 ggcgacgcct ggctcttctg cgacgggccg gtgcatccca gtggccggga ggagctcgtt
       61 tttggccagc gaggcgtcac cggcttcgag ctcacgatct acgggccgac gcgtcccctg
      121 cacagtggcc attatggcaa ctgggctccc aaccccggca tcctgctggc cagtctgatc
      181 gcgagcatgc gggatcccga cggccacatc aagatcgctg gctactacga caacgttcga
      241 ccgatcacgg ccgccgagca caaggcgatt gccgcgctgc ccgagtacga cgcgacgctc
      301 cgccgctccc ttggcctggc gcgcaccgaa gcgaacgacg ctccgctcgc cgaacggatc
      361 atgctccccg cgctcaacct gcgcgggatg cgcgtcggag gcgtgcgcga gacgggctcg
      421 aatacgatcc cgagtgtcgc ctatgcatcg gtcgaccttc gcctcgtgcc taacgaaaca
      481 ccggcacgcg tgcagcatct cgtggaagag cacatccgac ggcagggatt ctatctgacg
      541 cacgatagcg cgaccacggc cgagcgcctg gcacacccga agatcgcgcg aatcgagtgg
      601 gagagcggct accccgcgtc ccgtgagccg atggacggcc cgtttgggcg cgccgtgttg
      661 gcgaccctga acgacggcgc gccggcgccg ctgctcgccc tgccaaccat gggtggaagc
      721 ggaccgacgt acctgttcga gcagatcctg cacgtcccca tgatcacgct cccgatcgcc
      781 aattacgacg acaaccagca cgcggccgac gagaacctgc gactgcagaa tctctggaac
      841 ggcatcgagc tgtacgcgac gctgctctcc gggatcggcc ggcattgggg tgcggcgacc
      901 atgccgtgac cgtgaaaccg aacgttcgcc acgacatgaa cacgtccgcg ctagcggacc
      961 gcctgcattc gctggcgatc catctactgc ggcgcattcg acgcggcgac gaagaggtcg
     1021 gtttgagcgc gc
//
LOCUS       DASUCJ010001186         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1250749, whole genome shotgun sequence.
ACCESSION   DASUCJ010001186 DASUCJ010000000
VERSION     DASUCJ010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250749"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..986)
                     /locus_tag="VFU90_13865"
     CDS             complement(171..986)
                     /locus_tag="VFU90_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480927.1"
                     /translation="MTVRGYRAACVVLAATLSLMGATACGGGSGSQKQDAAAAHQVVA
                     TVNGTPITRAQVNHWMATLAGRDYYAVSHRLTIPAGLVSDPPNYAGCVAKLESVAAHA
                     SETSVQLLGKCRQLYQALRIQATEYLVGALRGIGMAHELGVDVSDATATRRYQELAAR
                     EYPSQAAYHRYLASTGESVSDGVLETKLELIGTAMLKKLNNPTGHVRYALAEQRWASK
                     VDCTAGYVVEYCKQYKGGRTYPTPPPSVLMEQTAALVTGRCINLAACREQAQK"
     gene            complement(1088..>1676)
                     /locus_tag="VFU90_13870"
     CDS             complement(1088..>1676)
                     /locus_tag="VFU90_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480928.1"
                     /translation="KACTEAQFNANPAGCPEGSNVGTATAITPVLNVPLTGPAYLVSH
                     GAAAFPDLEFVLQGEGVTIVLDGKTDIKKGITYSKFEAVPDAPISSFETSLPEGPHSV
                     LTTNTSLCAPVREKTVKKRVSRRVNGRVRKVTVSVKQLAPEALVIPTTLVGQNGVTVT
                     QSTKIAVTGCPKVKAKAHKASNRSKSKRGAKKRKK"
BASE COUNT          231 a          549 c          560 g          336 t
ORIGIN      
        1 tgcgccacgc cgcagccgca tattgcgcac agcggcacgg cgagcgcacc ggcgagcagg
       61 tggcggccgc ggccgctcat cgaagcgcga gcgacgccgc gatcgcggcg ctcacctcgt
      121 cgtggcgctc gcgtctgacg cgctggcacg cgcaggcgcg cacgatgcgc tcacttctgc
      181 gcctgctcgc gacaggcggc gaggttgatg cagcggcccg tcacgagcgc cgcggtctgc
      241 tccatcaaga cggacggcgg cggcgtcggg tacgttcggc cgcccttgta ctgcttgcag
      301 tattcgacga catagccggc cgtgcagtcg accttcgagg cccaccgctg ttcggcgagc
      361 gcgtagcgca cgtgcccggt cggattgttc agcttcttca gcatcgccgt tccgatcagc
      421 tcgagcttcg tttcgagcac gccgtcggag acgctttcgc cggtgctcgc cagatatctg
      481 tggtaagcgg cctgcgatgg gtattcacgc gccgccagct cctggtagcg gcgtgtcgcc
      541 gtcgcgtcgc tgacgtcgac gccgagttcg tgtgccatgc cgatgccgcg caatgcgccg
      601 acgagatact ccgtcgcctg gatgcgcagc gcctggtaga gctgcctgca tttgccgagc
      661 agctggactg aggtttcgct cgcgtgcgcc gcgaccgatt cgagctttgc gacgcagccg
      721 gcatagttgg gcggatcgga gaccaagccg gcgggaatgg tcagccggtg tgacaccgcg
      781 tagtagtcgc gtcccgcaag cgtcgccatc cagtgattca cctgcgcgcg ggtgatcggc
      841 gtgccgttca ccgtcgcgac gacctggtgc gccgcggcgg cgtcctgctt ctgactaccg
      901 ctgccgccac cgcatgccgt cgcgcccatg agcgagagcg tcgccgcgag cacaacgcag
      961 gccgcccgat atcccctcac cgtcatacgt cgatcctatg cacgcgatcc ggatttgtgt
     1021 tgctctgttt ctagacattt cggatattcg cggatgctgt gtgctgcatt tgaaagctgc
     1081 cgagcagcta cttcttgcgc ttcttcgcgc cgcgcttgga cttcgaccgg ttcgaagcct
     1141 tgtgcgcttt cgccttgacc ttcgggcagc cggtcacggc gatcttcgtg ctctgcgtga
     1201 cggttacgcc gttctgaccg acgagcgtcg tcgggatcac gagcgcttcg ggcgcgagct
     1261 gtttgaccga gacggtgact ttgcgcacgc gaccgttcac tcggcggctg acgcgcttct
     1321 tgacggtctt ttcgcgcacc ggtgcgcaga ggctcgtgtt tgtggtgagc accgagtgcg
     1381 gtccctcggg caggctcgtt tcgaacgagc tgatcggcgc gtcgggcacg gcttcgaatt
     1441 tcgagtacgt gatgcccttt ttgatgtctg tcttgccgtc gagcacgatc gtcacgcctt
     1501 cgccctgcag cacgaactcg aggtcgggga aggcggcggc gccgtgcgag acgaggtacg
     1561 ccggaccagt cagcggcacg ttcagaaccg gcgtgatcgc cgtcgcggtg ccgacgttcg
     1621 agccttcggg gcagccggcg ggattcgcgt tgaactgcgc ttcggtgcac gctttc
//
LOCUS       DASUCJ010001187         1374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_104565, whole genome shotgun sequence.
ACCESSION   DASUCJ010001187 DASUCJ010000000
VERSION     DASUCJ010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104565"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..964
                     /locus_tag="VFU90_13875"
     CDS             137..964
                     /locus_tag="VFU90_13875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5480929.1"
                     /translation="MEIGVATFPTHDSLGPAQVAQLAEEHGYESLFFTDHTHIPASRE
                     TPYAGGVPLPRKYAHIYDMFVALTAAAVVTTKIRIGSGVCLVIERDPIQTAKEVASVD
                     HLSGGRFEFGVGAGWNREEMANHGTDPRRRMSVLRERIEAMKQIWTQDEASYSGDFVK
                     FERIWSWPKPLQRPHPPVLVGGGGPTVFDRVLDYGNAWFPNYAPDVIDRAADLRRRAE
                     RPIDLMVMGIPADPAIIERFEIAGFRRVVHWLPSAGRSVVEREMERFESAVDEVHGH"
     gene            966..>1374
                     /locus_tag="VFU90_13880"
     CDS             966..>1374
                     /locus_tag="VFU90_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5480930.1"
                     /translation="MRLDDVEARRRFANARVARLATVDEAGRPHIVPVVFVVIEQHIY
                     SAVDAKPKTTKALRRLANISANPRVSLLTDHYADGDWDALWWVRADGEARVIAPDAPE
                     AEAAVDGLIGRYSQYREHRPDGPVLAVAVRRWRG"
BASE COUNT          230 a          452 c          466 g          226 t
ORIGIN      
        1 cgactgaagg ttttgcgctg ctgagttcgc caatttgacc gcgcaacccc ttcactcggc
       61 gcggagcgac ggagcgctgg tcgcggcgat caagggggtc gggtggggtg ttccgcggcc
      121 gggctagggt cggatcatgg agatcggtgt cgccactttt cccacccatg actcgctcgg
      181 cccggcccag gtcgcgcagc tcgccgagga acacggttat gagtcgctct tcttcaccga
      241 ccacacccac atcccggcca gtcgcgagac gccctacgcg ggtggcgttc cgctgcctcg
      301 caagtacgcg cacatctacg acatgttcgt ggccctcacc gccgctgcag tagtcacgac
      361 gaaaatccgg atcggcagcg gcgtgtgcct ggtcatcgag cgcgatccga tccagaccgc
      421 gaaggaggtc gcgagcgtcg atcacctctc cggcggccgg ttcgaattcg gcgtgggcgc
      481 cggctggaac cgcgaggaga tggccaacca cggcaccgat ccgcggcggc gcatgagcgt
      541 gttgcgcgag cggatcgagg cgatgaagca gatctggacg caagacgaag ccagctactc
      601 cggtgacttc gtgaagttcg agcggatctg gtcgtggccg aaacccctgc agcggccgca
      661 cccgccggtg ctcgtcggcg gtggcggccc gacggtcttc gatcgggtgc tcgactacgg
      721 caacgcctgg ttcccgaact acgcgcccga cgtcatcgac cgcgcggccg atctgcgccg
      781 ccgcgccgag cgaccaattg accttatggt gatgggaatt cccgccgacc ccgcgatcat
      841 cgagcgattc gagatcgccg gatttcggcg cgtcgtgcat tggcttccgt ccgccggtag
      901 atccgttgtc gaacgcgaga tggagcgatt cgagtcggct gttgacgagg tgcacgggca
      961 ctgagatgag gctggacgac gtcgaggcgc gccgccgttt tgccaacgcg cgagtggcgc
     1021 ggctcgcgac cgtcgacgag gcgggacgtc cgcacatcgt gccggtcgtg ttcgtcgtga
     1081 tcgaacagca catttacagc gcggtcgacg caaaaccaaa gaccaccaag gcactgcgcc
     1141 gactcgcgaa catcagcgcc aacccgcgtg tgagcttgct caccgatcat tacgcggacg
     1201 gcgattggga cgccttgtgg tgggtgcgcg ctgacggcga ggcccgcgtg atcgcccccg
     1261 acgcacccga ggccgaagcc gccgtcgacg gcttgatcgg ccggtattcg caataccgcg
     1321 agcaccggcc cgacgggccg gtgctcgcgg tcgccgtccg aagatggcgc ggat
//
LOCUS       DASUCJ010001188         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1067229, whole genome shotgun sequence.
ACCESSION   DASUCJ010001188 DASUCJ010000000
VERSION     DASUCJ010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067229"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFU90_13885"
     CDS             <1..520
                     /locus_tag="VFU90_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480931.1"
                     /translation="NFDYDALLEKAQPYFVVMRRDDAPTAETLLEWLAKEAPTHPAFV
                     VAHGKRAALLAAKKNVDFISDVHPALRETDVAMLHSGVLEHLLGISKEAQAKKMNIRY
                     PQDARAALAELRAGTGNAQALFLMNATPVAQVRAVAEAGEVMPQKSTFFHPKVPTGIL
                     LHTLDPSRSIPR"
     gene            530..>1069
                     /locus_tag="VFU90_13890"
     CDS             530..>1069
                     /locus_tag="VFU90_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480932.1"
                     /translation="MRKAILLALVLASASAHATEESTRRPFFIQGTLGSVGVWAWTNP
                     PGVLGYWHPDFEFGYHFSGRHDGVEIGLRQGFYLPHDRYDVGETLLRGGYDFAFPFRN
                     GRFELTVAPFGTFGINYLFTNLNAGIHASVGIEAKLFFFHDLYLLVRPVELAIGEYVN
                     LGPTTANVFFNMNAGIGAGL"
BASE COUNT          186 a          384 c          308 g          191 t
ORIGIN      
        1 gaatttcgac tacgacgcgc tgctcgaaaa agcgcagccg tatttcgtcg tgatgcggcg
       61 cgacgacgcg cccaccgccg agacgctgct cgagtggctc gcgaaggaag cgccgactca
      121 tcccgcgttc gtcgtcgcgc acggaaaacg agccgcgctc ctcgcggcga agaagaacgt
      181 cgatttcatc tccgacgtcc accccgcgct gcgcgagacc gacgtcgcga tgctccactc
      241 cggcgtcctc gagcacctgc tcggcatctc gaaagaggcg caggccaaaa agatgaacat
      301 ccgctacccg caggacgcgc gcgccgcgct cgccgaattg cgcgccggga ccggaaacgc
      361 gcaagctttg ttcctcatga acgcgacacc cgtcgcccaa gtgcgcgccg tcgccgaggc
      421 cggcgaggtc atgccccaga agtcgacctt cttccacccc aaggtcccga ccggcatcct
      481 ccttcacacg ctcgatccgt cgcgctcgat tccgcgatag aacgggttca tgcgaaaggc
      541 gatcctcctc gcgctcgttt tggcgagcgc gtcggcgcac gcaaccgaag aatcgacgcg
      601 ccgtccgttc ttcatccaag gcacgctcgg ctcggtcggc gtgtgggcgt ggaccaaccc
      661 gcccggcgtg ctcggctact ggcaccccga tttcgagttc ggctaccact tcagcgggcg
      721 ccacgacggc gtcgagatcg gcctgcgtca gggcttttat ctcccgcacg atcgctacga
      781 cgtcggcgag acgctcttgc gcgggggtta cgatttcgcc ttcccgttcc gtaacgggag
      841 gttcgagctc acggtcgcgc cgttcggcac gttcggcatc aattatctct tcacgaacct
      901 caacgccggc atccacgcga gcgtcggcat cgaggccaag ctatttttct tccacgacct
      961 ttacttgctc gttcgccccg tcgagctggc gatcggcgaa tacgtgaacc tcggccccac
     1021 gaccgccaac gtctttttca acatgaacgc cggcatcggc gccggcctg
//
LOCUS       DASUCJ010001189         1360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1311646, whole genome shotgun sequence.
ACCESSION   DASUCJ010001189 DASUCJ010000000
VERSION     DASUCJ010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311646"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..214)
                     /locus_tag="VFU90_13895"
     tRNA            complement(142..214)
                     /locus_tag="VFU90_13895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Gly,seq:gcc)
     gene            complement(297..>1360)
                     /locus_tag="VFU90_13900"
     CDS             complement(297..>1360)
                     /locus_tag="VFU90_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480933.1"
                     /translation="GINDSTQRQAYPVLLARQMNTPFTVASLANPGCPPPIDSFPEGT
                     RVGGGTSTTCSFRSQVAGVAVLNNVAVPGAASFDPASPIGTGGGGPLTTFILGGKTQV
                     QRALDAKPTFVSIWIGNNDVLPAALSGILDSVPGVSPGVTSEAAFEQNYNAMMTGLAA
                     GTTIKGGILIGVVNVSNAPIFFPAPALFAPGVLPELEAAVGHSITVDTSCTPTTQSVI
                     NFELLESMRAGTAPDTIACHALANHANGLGEVFVLDATEIAAVTQAVAGYNAFIQQTA
                     ANKGWAYFDPNPTLEQLRESGQIPVLPDITQPTKAFGDFISLDGIHPAAAAHVVLANL
                     MIQAINTQYSTTIPALPSP"
BASE COUNT          230 a          433 c          463 g          234 t
ORIGIN      
        1 ctaggggaaa gggattttaa gtccctcgcg tctgccgttt cgccacccgg gcccagaggc
       61 tcgcacgacc accctggaac aacacaggga caccggctgg gcgccgcatg tccctgtgat
      121 cgaatcgcac gagtcgtgga gagcgggaaa cgggactcga acccgcgacc ccaaccttgg
      181 caaggttgtg ctctaccaac tgagctattc ccgcagtcgc cacaaagcta gtccaagcct
      241 cgcggtcgaa caagaggacg cggccgctcg ctgcctgtac gcgacgtcgc cgtgcgtcac
      301 ggcgatggca acgccggaat cgtcgtgctg tactgcgtgt tgatcgcttg aatcatcagg
      361 ttggccaaca ccacgtgcgc ggccgcagcc gggtgaatgc catcgagcga gatgaaatcg
      421 ccgaacgctt tcgtgggttg cgtgatgtcg ggcagcacgg gaatctgtcc actctcgcgc
      481 agttgctcca gcgtgggatt cggatcgaag tacgcccagc ccttgttcgc ggcggtctgc
      541 tggatgaatg cgttgtatcc ggcgacggct tgcgtcacgg cggcgatctc ggtcgcgtct
      601 aacacaaaca cctcgccgag gccgtttgcg tgattggcga gcgcgtggca ggcgatggtg
      661 tcgggcgccg tgccggcccg catcgactcg agcagttcga agttgatcac cgactgggtg
      721 gtcggcgtgc agctcgtgtc gacggtgatc gaatggccga ccgccgcctc gagctccggc
      781 aagacgccgg gcgcgaacag cgccggcgcc gggaagaaga tcggtgcgtt agacacgttc
      841 acgacgccga tcaagatgcc gcccttgatc gtcgtcccgg cggcgaggcc ggtcatcatc
      901 gcgttgtagt tctgctcgaa cgccgcttcg ctggtcacgc ccggcgacac gccgggcacc
      961 gagtcgagga tgccgctcag ggcggcgggc aggacgtcgt tgttgccgat ccagatgctc
     1021 acgaacgtcg gcttcgcgtc taacgcgcgc tgcacctggg tcttcccgcc gaggatgaac
     1081 gtcgtcagcg gaccgccgcc gccggtgccg atcggactcg ccggatcgaa cgaggcggcg
     1141 ccgggcacgg ccacgttgtt cagcacggcg actccggcaa cctgcgatcg gaacgaacat
     1201 gtggtcgacg tgccgccgcc gacgcgcgtg ccttccggaa aactgtcgat cggcggcggg
     1261 cagccggggt tggcaaggct ggccaccgtg aagggcgtgt tcatctgccg cgcgagcaac
     1321 accgggtagg cttgccgctg cgtggagtcg ttgatgccgc
//
LOCUS       DASUCJ010001190         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_654655, whole genome shotgun sequence.
ACCESSION   DASUCJ010001190 DASUCJ010000000
VERSION     DASUCJ010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654655"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..806
                     /locus_tag="VFU90_13905"
     CDS             15..806
                     /locus_tag="VFU90_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5480934.1"
                     /translation="MDLGLEGRVALVTGASSGLGRAVARELAMEGASVAIVGRHSETL
                     NRTASEISAESRRRVLPLTADVTDGPAIIAAVKEAKQTLGPIDILVANAGGPPATDIE
                     ETTEAQYRAAIEVNLFASIRLSYQVVAGMRRRQWGRIIFLTSTAAKQPIQSLVLSNTA
                     RAGLAAFAKTLATDCAKDNVLVNTILTGHFDTDRSMSLAAGRARRENRSLDEVLVERR
                     RRIPMGRSGHPGELASVVAFLASERASFVTGTAIAVDGGQIGTVF"
     gene            945..>1742
                     /locus_tag="VFU90_13910"
     CDS             945..>1742
                     /locus_tag="VFU90_13910"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5480935.1"
                     /translation="MRALVKETAAPGFTLKDVPEPTIRDDEVLIRVRRAGVCGTDVHI
                     YEWDDWARGRCHPPFIVGHEFAGDVARVGSLVVDVHVGDRVTAEGHIVDGRCLLCRTG
                     NAHACPYTKVIGVDRDGCFAEYIAMPATNVWHLDDDISYDIGGIHDPMGNAFHTALTA
                     EIPGATVLITGCGPIGIFAVGICHAAGASRIIASDVNETRLALAKQMGADDVVHPDEL
                     ERTVKRHTDGLGVDVVLEMSGVPSAIHQAFALVRRAGRVQMLGVPAKP"
BASE COUNT          306 a          505 c          615 g          316 t
ORIGIN      
        1 cgggagaggc ttgagtggat ctcgggttag agggaagagt cgcgctcgtc acgggtgcat
       61 cgagcggact cggccgggcg gttgcgcgcg agctcgcgat ggaaggcgca tcggtggcga
      121 tcgttgggcg acacagcgaa acgttgaatc gaacggcgag cgagatctcc gctgaatcac
      181 gacgacgtgt gctgccgttg acggcagatg tcacggatgg tcccgcgatc atcgcggcgg
      241 tgaaggaagc aaagcaaacg ctcggcccga tcgacattct cgtggcaaat gcgggtggtc
      301 cccccgccac ggacatcgaa gagacgaccg aagcgcagta tcgcgcggcg atcgaggtca
      361 acctgttcgc atcgatccgt ctctcgtatc aagtggttgc cggcatgcgc cgccgccaat
      421 ggggacgcat catcttcctg acgtcgaccg cggcgaagca accgatacaa tcgctggtgc
      481 tctcgaacac ggcgcgtgcg gggttggcgg cgttcgccaa gacgctggct acggactgcg
      541 cgaaagacaa cgtgctcgtg aacacgattc tcacaggaca cttcgacacc gatcgctcga
      601 tgtcgttggc cgccgggcgc gcgcggcgcg aaaatcgttc gctcgacgaa gtactcgtcg
      661 aacgacggcg caggataccg atgggtcgtt cgggtcatcc gggcgagctc gcatcagtgg
      721 tggcgtttct cgcgtcggag cgggcgagct tcgtgaccgg aacagccatt gccgtcgatg
      781 gcggtcagat cggcacggtg ttctgaacgg cgagcgacca acgggacgtc gtgcggagtt
      841 cccctggcgt catcggcggt ccagcgacag ctttcccgcg cgccggtgtg atgtggatgc
      901 cgtgtgaacg cggacgttga ccatcgattg gagccataga tcgcgtgaga gcgctcgtca
      961 aagagactgc agcccccggt ttcacgttga aggatgttcc cgaaccaacg attcgcgacg
     1021 acgaggtgct gattcgtgtg cgtcgcgcgg gtgtctgcgg gacggacgtg cacatctacg
     1081 agtgggatga ctgggcgcgc ggccggtgtc atccgccgtt catcgttggg cacgagttcg
     1141 ccggcgatgt cgcccgcgtc ggctcgttgg ttgtcgacgt ccacgtcggc gatcgggtga
     1201 cggccgaggg gcacatcgtc gatgggcgat gtctcctgtg ccggacgggc aatgcgcatg
     1261 cgtgtccgta cacgaaagtg atcggcgtcg atcgcgatgg ctgtttcgcg gaatacatcg
     1321 ccatgcccgc gaccaacgtg tggcatctcg acgatgacat ctcgtacgac atcggcggca
     1381 ttcacgatcc gatgggcaac gcgttccaca ctgcgctcac ggcggagatt cccggcgcga
     1441 cggtgttgat cacggggtgc gggcccatcg gcatcttcgc ggtcgggatc tgccacgcgg
     1501 ccggcgcatc gcgaatcatt gcgtcggatg tgaacgagac gcgactggcg ctcgcgaagc
     1561 agatgggtgc cgacgacgtg gtgcatccgg acgagctcga acgcacggtg aagcgtcaca
     1621 cggatggact cggcgtggac gtcgtgttgg agatgtcggg ggttccgtcg gccattcatc
     1681 aagcgttcgc gctcgtgcgg cgggcgggtc gcgtacagat gctcggtgtg ccggccaagc
     1741 cc
//
LOCUS       DASUCJ010001191         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_165451, whole genome shotgun sequence.
ACCESSION   DASUCJ010001191 DASUCJ010000000
VERSION     DASUCJ010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165451"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU90_13915"
     CDS             <1..198
                     /locus_tag="VFU90_13915"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU5480936.1"
                     /translation="IDRDFRHEGVLGTVFTGRLVAETTVGPYRAVVPTLSGQGWITGF
                     SSYVVDPTDPFPEGFTVGDIW"
     gene            complement(230..>1300)
                     /locus_tag="VFU90_13920"
     CDS             complement(230..>1300)
                     /locus_tag="VFU90_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480937.1"
                     /translation="VHETEAWLSQADVTTPPWRLARDTTLASAVAPHFEHLGRAYGLT
                     QWDARADVPAWLRRTSLVLTLHGMHYTGYVFNDFARMLEIVRWTAAQIGGDRVLAFLP
                     SWDGRYYWDYPGYRAADRLGGEAGLRRLVQAGHDLGVRFMPMFGANAANRHAAGFDTI
                     AGAATAKIDGDPLDLNWVDWDNDRHQEGWLSYMNLGVESWRSWLTQRIADAIERYGVD
                     AYFLDIVGGWVNNPRADMHDGTRRLVAGIARAHPQVLACGEMHYDALLAFIPLYQSFS
                     QWEVRDQVQRYARFFQHLSHPAPGRGSSGVHESGFNHWDPRTLSLRAGQIPTLNVVDD
                     TFSRYRTEMAAVIAKARALAGA"
BASE COUNT          216 a          466 c          410 g          208 t
ORIGIN      
        1 atcgatcgcg acttccggca cgaaggcgtg ttaggcaccg tgttcaccgg acgcctcgtg
       61 gccgaaacga cggtcggacc gtaccgcgcc gtcgtgccca cactcagcgg ccaaggctgg
      121 atcaccggat tcagctccta cgtggtcgac ccgacggatc cgttccccga gggcttcacc
      181 gtcggggaca tctggtagca cgcgcgagcg gccacgcccc cgatcagcgt taggcgccgg
      241 cgagcgctct ggccttcgcg atcaccgccg ccatctcggt tcgatacctc gaaaacgtgt
      301 cgtccaccac gttgagtgtc gggatctgcc ccgcgcgcaa cgacaacgtg cggggatccc
      361 agtgattgaa ccccgattcg tgcacgccgc tgctgccgcg gcccggcgcc gggtggctca
      421 gatgctggaa gaagcgcgcg tagcgctgca cctgatcgcg cacctcccat tgcgagaacg
      481 actgatagag cggaatgaac gccagaagcg cgtcgtagtg catttcgccg cacgccaaca
      541 cttgcggatg cgcacgcgcg atgccggcaa ccagccggcg tgtgccgtcg tgcatgtcgg
      601 cccgcgggtt gttcacccac ccgccgacga tgtcgaggaa ataggcgtcc acgccgtatc
      661 gctcgatcgc atccgcgatc cgctgcgtga gccacgagcg ccacgactcc acacccaggt
      721 tcatgtacga cagccagccc tcttgatggc ggtcgttgtc ccagtcgacc caattcaggt
      781 ccagcggatc gccatcgatc tttgccgtcg cggcgcccgc gatcgtgtcg aagccggccg
      841 cgtgccggtt ggccgcattt gcgccgaaca tcggcatgaa gcgcacgccc aggtcgtgtc
      901 cggcctgcac cagcctccgg agcccggcct cgccgcctaa ccgatcggcc gcgcggtagc
      961 ccggatagtc ccagtagtac cgcccgtccc acgacggcag aaacgccagc acccgatcgc
     1021 cgccgatctg cgccgcggtc caccggacga tctcgagcat ccgcgcgaaa tcgttgaaca
     1081 cgtatccggt gtaatgcatc ccgtgcagcg tgagcacgag cgacgtgcgc cggagccagg
     1141 ctggtacgtc ggcccgcgca tcccactgcg ttaggccgta cgctcggccc agatgctcga
     1201 agtgaggcgc gaccgcgctg gcgagcgtgg tgtcgcgcgc caggcgccac ggcggcgtcg
     1261 tgacgtccgc ctgtgagagc caggcttccg tttcgtgcac
//
LOCUS       DASUCJ010001192         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_700143, whole genome shotgun sequence.
ACCESSION   DASUCJ010001192 DASUCJ010000000
VERSION     DASUCJ010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700143"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1034
                     /gene="speE"
                     /locus_tag="VFU90_13925"
     CDS             165..1034
                     /gene="speE"
                     /locus_tag="VFU90_13925"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU5480938.1"
                     /translation="MSADKKTTEHDWFTERHEHSGSSIGFRVKARLHSEKTPFQTIEI
                     YDSTDWGKLMVIDGCMMVTTRDNFFYHEMMTHPALFTHARAKRVVVIGGGDCGTLREV
                     LKHDEVEKATQVEIDERVTRLAEQYFPELCESNGDPRAELLFIDGIKYMKDCEPESLD
                     LVIVDSTDPVGPAEGLFNADFYKSCFEALKSSGILVQQSESPLAHLDLIKSMRTAMRM
                     ADFQSVRTLTFPQPCYPTGWWSCTMARKNADLDGFRERGAQTKQFDTRYYNAEIHKAA
                     LAMPEFVREVLGD"
BASE COUNT          222 a          405 c          384 g          196 t
ORIGIN      
        1 gtcgcagcgg cgggccttga cggattgaac ccctcggtcg gaagcctgcg acgccgcggg
       61 acccaggtcc gcgtattgta acgagccctg ttgcgcgtgc atgtccgtgt caggcggaac
      121 cggtacaatc cgcgcccccg cccgaaccgc cgcaggaagc cgcgatgtcc gccgacaaga
      181 agaccaccga gcacgactgg ttcaccgaac ggcacgaaca ttcgggctcg tcgatcgggt
      241 ttcgcgtcaa ggcgcgcctg cactcggaaa agacgccgtt ccagacgatc gagatctacg
      301 actcgaccga ctggggcaag ctcatggtca tcgacggctg catgatggtc acgacgcgcg
      361 acaacttctt ctatcacgag atgatgacgc accccgcgct gttcacgcat gcgcgcgcca
      421 agcgcgtcgt cgtcatcggc ggcggcgatt gcggcacgct gcgcgaagtg ctcaagcacg
      481 acgaagtcga gaaggcgacg caggtcgaga tcgacgagcg cgtgacgcgg ctcgccgagc
      541 agtattttcc cgagctttgc gaatcgaacg gcgatccgcg cgccgagctc ctcttcatcg
      601 acggcatcaa gtacatgaag gactgcgagc cggaatcgct cgatctcgtc atcgtcgatt
      661 cgaccgatcc ggtcggcccg gccgaagggc ttttcaacgc cgatttctac aagagctgct
      721 tcgaggcgct caaatccagc ggcattctcg tgcagcagtc cgaatcgccg ctcgcgcacc
      781 tcgacctcat caagtcgatg cgcacggcga tgcgcatggc cgatttccag tcggtgcgga
      841 cgctgacgtt cccgcagccg tgctacccga ccggctggtg gagctgcacg atggcgcgca
      901 agaacgccga cctcgacggc ttccgcgagc gcggcgcaca gaccaagcag ttcgacacgc
      961 gctactacaa cgccgagatc cacaaggccg cgctggcgat gccggagttc gtgcgcgaag
     1021 tgctcggcga ctaggcctgc gctcacgcac cgtcgaatcc gtcggcgaag atctgctccg
     1081 cgttcgccgg cgcgtgctcg tacgcgccga tgtcgatgtg cacgccttcg atgcgcgtga
     1141 cgccgtcgag gtcgaacccg gcgacgccgc cggctggcgt gtcggtgccg gcgtcgatca
     1201 gcggcga
//
LOCUS       DASUCJ010001193         1382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_74017, whole genome shotgun sequence.
ACCESSION   DASUCJ010001193 DASUCJ010000000
VERSION     DASUCJ010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74017"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VFU90_13930"
     CDS             complement(<1..1367)
                     /locus_tag="VFU90_13930"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5480939.1"
                     /translation="MTWNSAFKRVGILDAIQVQDQPDDPNWDPEDLRHNMVRWFDSSS
                     PQYGAEALIVNDPRTGEEINAGINIDSVVATGYNAAYTYWVAPTRGVPDDPTARKAFA
                     LQNLRALVLHESGHDMGLQHNFIGSMAYTAKELQSKAFTDKMGIASSVMEYAPLNLWP
                     KGTPQGDWAQLVLGPYDYHAIDYGYTYIPASTPQQELPTLRRIASQWATPELSFASDE
                     DVSWPGGHAIDPRVQQFDLTNDPLGWCGVQMNFDQADMNAVVARFPRYEQPNQDARQA
                     FGVPFRQYARCASMTAHVIGGEYLSRGRRGDPGEKPGLMAVSRSDEARAWHLLDQYLF
                     SDGAWHFNPTVLTQLTYTEQSTIGGFATWAYNPGGRHDVDVVGLAGGAQDAALSEFFA
                     PLTLSRIDDLQTKYAPGKTMTITDLFDWAQASIYGDIANGRAASDGVVMRNLQARYTK
                     RLGAL"
BASE COUNT          211 a          456 c          462 g          253 t
ORIGIN      
        1 cacagcgcgc cgagccgctt cgtgtagcgc gcctgcaggt tgcgcatgac gacgccgtcc
       61 gacgccgcac gaccgttcgc gatgtcgccg tagatcgacg cctgcgccca gtcgaagagg
      121 tcggttatcg tcatcgtctt ccccggcgcg tacttcgtct gcaggtcgtc gatgcgcgac
      181 agcgtcagcg gcgcgaagaa ctccgacagc gcggcatcct gcgccccgcc ggcgagcccg
      241 acgacatcga cgtcgtgacg gccgcccggg ttgtacgccc acgtcgcgaa gcccccgatt
      301 gtgctctgct ccgtgtaggt cagttgcgtg agcaccgtcg ggttaaaatg ccacgcgccg
      361 tccgagaaga gatactggtc gaggagatgc caggctcgcg cctcgtcgct gcgcgacacc
      421 gccatgaggc ccggcttctc gcccggatca ccgcggcgac cgcgcgagag atattcgccg
      481 ccgatgacgt gcgctgtcat cgacgcgcaa cgcgcgtatt ggcggaacgg cacgccgaac
      541 gcttgacgcg cgtcttggtt cggctgctcg tagcgcggga aacgcgcgac cactgcgttc
      601 atatccgcct ggtcgaagtt catctggacg ccgcaccacc cgagcgggtc gttcgtgagg
      661 tcgaactgct ggacgcgcgg atcgatcgcg tgtccgccgg gccacgacac gtcctcatcg
      721 gacgcgaagc tcagttccgg agtcgcccac tgcgaggcga tgcgccgcag cgtcgggagt
      781 tcttgctgcg gcgtgctcgc ggggatgtac gtgtagccgt agtcgatcgc gtgataatcg
      841 tacggcccga gcacgagctg cgcccagtcg ccttgcggcg tgcccttcgg ccatagattg
      901 agcggcgcgt actccatgac ggacgacgcg atgcccatct tgtcggtgaa cgccttcgac
      961 tggagctcct tcgcggtgta ggccatcgac ccgatgaagt tgtgctgcag gcccatgtcg
     1021 tgtccggact cgtggaggac gagcgcccgc aggttctgca gcgcgaacgc cttgcgcgcc
     1081 gtcgggtcat cgggcactcc gcgcgtcggc gcgacccagt acgtgtaggc cgcgttgtag
     1141 ccggtcgcga cgaccgagtc gatgttgatg cccgcgttga tctcttcgcc ggtgcgcgga
     1201 tcgttgacga tgagggcctc cgctccgtat tgcggcgacg acgagtcgaa ccagcggacc
     1261 atgttgtgcc gcaggtcttc cggatcccag ttcggatcgt ccggctggtc ctggacttgg
     1321 atcgcgtcga ggatgccgac gcgcttgaac gcggagttcc acgtcaagag cgcatccttg
     1381 at
//
LOCUS       DASUCJ010001194         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1128299, whole genome shotgun sequence.
ACCESSION   DASUCJ010001194 DASUCJ010000000
VERSION     DASUCJ010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128299"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VFU90_13935"
     CDS             <1..1352
                     /locus_tag="VFU90_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480940.1"
                     /translation="SPNELTGCVRARPSEDPNSALFRIEVIKVPLANPEQAAIVSSPR
                     IFNDLVAPQHHGEAPADKAALEEARAKGAFIVNIPILGEEMVMPPRFVNPMLDSVVKA
                     RGGTGAPTAADSAALREALPDLIAKMFGANAGSGPRPGPTQCHDITVYPAIGFAGGAC
                     EGYGMLLDIHDPEHPVRLYAASDSNFSYWHSATFNNDGTKLLFSDEWGGGGQPKCRAT
                     DPKQWGGDAIFSIEGNALHFQSYYKMSAPQTSFENCVAHNGSLIPIPGRDVMVQSWYQ
                     GGISVFDFTDAAHPKEIAFFDRGPINGTRMEDGGTWSAYWYNGVIVSSEIARGLDILE
                     LTPSPLLTQNEIDAAKSVHLTYLNVQGQPKYTWPPSFALARAYVDQLERSNGLSADQL
                     KSVRQSLGSAEQASGDSRKTALTQLASQIEGDANGSSDAAKVHTLASAVRDLAGGGM"
     gene            1445..>1905
                     /locus_tag="VFU90_13940"
     CDS             1445..>1905
                     /locus_tag="VFU90_13940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5480941.1"
                     /translation="MHASQPAVALLLAAVAFVAATLAAVTGFGGAAVLLPVLIAVFGT
                     REAIPILTVAQLIGNGSRVWFNRREVDWRVVGWFAIGAVPFAFAGGLLFARAPLQPLT
                     RLLGAFLILVVVWRRVHPGPPRRPPIRSFAVIGAGASFLSALLGSVGPIMAP"
BASE COUNT          300 a          617 c          652 g          336 t
ORIGIN      
        1 gttcgcctaa cgaactgacc ggctgcgtgc gggcccgtcc gtcggaggat ccgaactcgg
       61 cgttgttccg catcgaggtg atcaaggttc cgttggcgaa cccggagcag gccgccatcg
      121 tgagctcgcc gcgcatcttc aacgatctcg tcgccccgca gcatcacggt gaggcgcccg
      181 cggacaaagc ggcgctcgag gaggcgcgtg ccaagggagc gttcatcgtg aacattccga
      241 tcctgggcga agagatggtg atgccgcccc ggttcgtgaa tccgatgctg gacagcgtcg
      301 tgaaggcgcg cggcgggaca ggcgcaccaa cggccgccga cagcgccgcg ttgcgcgaag
      361 cgctgccgga cttgatcgcg aagatgttcg gagcgaacgc gggcagcggt ccgcgtcccg
      421 gtccaacgca atgccacgat atcacggtgt atccggccat cggattcgcc ggcggcgcgt
      481 gcgaaggcta tgggatgctg ctcgacatcc acgaccccga gcatcccgtt cgtctctacg
      541 ccgcgtccga ctcgaacttc tcctactggc actcggcgac gttcaacaac gacggcacca
      601 agttgttgtt ctccgatgaa tggggcggcg gcggccagcc caagtgccgc gcgaccgatc
      661 ccaagcagtg gggcggcgac gcgattttct cgatcgaagg gaatgcgctg cacttccaga
      721 gctactacaa gatgtccgcg ccgcagacgt cgttcgagaa ttgcgtcgcg cacaacggct
      781 cgctcattcc gattccgggc cgcgatgtga tggtgcagtc ctggtaccag ggcggaattt
      841 cggtgttcga cttcaccgac gccgcgcatc ccaaggagat cgcgttcttc gaccgcgggc
      901 cgatcaacgg gacgcggatg gaagacggcg gcacgtggtc ggcatactgg tacaacggcg
      961 tgatcgtgag ctcggagatc gcgcgcggtc tcgacatcct cgagttgacg ccgagtccgc
     1021 tgctcacgca gaacgaaatc gatgcggcga agtcggtgca cctgacgtat ctcaacgtgc
     1081 agggccagcc caagtacacg tggccgccca gcttcgcgct ggcgcgcgcg tacgtcgacc
     1141 agctcgagcg gtcgaacggt ctgtccgcgg accagctgaa gtccgttaga cagtcattag
     1201 gcagcgccga gcaggcgtcg ggggactcga ggaaaacggc gttgacgcag ctcgcgtcgc
     1261 agatcgaggg cgatgcgaac ggctcgtcgg acgccgccaa ggtgcacacc ctggcgtcag
     1321 cggtgcggga tctggcgggc ggcgggatgt gatcgctggt gcctaacgcc acgcttcgtc
     1381 cgctgaagcg tgcaaacgac ccggtgcgct ggatgcgcgc cgggtcgttg catgcgtccc
     1441 gctgatgcac gcgtcgcagc cggcggtcgc gctgctgctt gccgcggtcg ccttcgtggc
     1501 cgccacgctt gccgccgtga ccgggttcgg cggcgcggcc gtgctgttgc ccgtgctcat
     1561 cgccgtcttc ggaacgcgcg aagccatccc tatcctcacg gtcgcgcagc tcatcggcaa
     1621 cgggagccgc gtctggttca atcgacgcga ggtcgactgg cgggtggtcg gctggttcgc
     1681 gatcggtgct gtgcccttcg cattcgccgg tggattgttg ttcgcgcgcg ccccgctgca
     1741 gccgctcacg agactgctcg gcgcgtttct gattctcgtg gtggtgtggc gtcgtgtgca
     1801 tccgggacca ccgcggcggc cgccgatcag aagcttcgcc gtcatcggcg ccggcgccag
     1861 tttcctttcc gcgctgctgg gcagcgtcgg gccgatcatg gcgcc
//
LOCUS       DASUCJ010001195         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_776726, whole genome shotgun sequence.
ACCESSION   DASUCJ010001195 DASUCJ010000000
VERSION     DASUCJ010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776726"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..906
                     /locus_tag="VFU90_13945"
     CDS             67..906
                     /locus_tag="VFU90_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5480942.1"
                     /translation="MPEAPQMQALSERIGAWLGGATFEGYEPLGFTGLKTYDPPPESL
                     VGARLERVDRRAKYVSMWFDNGLRVVFHLSQAGRVDFEEPPKRTKPKGAVARWRFSDG
                     RAVLLREHGTERKAGWWVLAPGDDGPLEGLGPEALSDEFATWLRTSDDGRRIHTILRD
                     QRTVAGVGRGYADDVLHRAKLSPYASLKSLSPDERERLIDALHEVLAEGLERERSREG
                     GLSQNKLGEHFTVHGKAGVPCPECGGDLKRVSYESHEVVYCPSCQTGGKVLADRRLSR
                     LIK"
BASE COUNT          173 a          326 c          401 g          173 t
ORIGIN      
        1 ggcgagcagt gaggcggggc ccgagcggcc cggcgtgcag cgccgtgagc ggaaaggtca
       61 tctgacgtgc cggaggcacc tcaaatgcag gccctgtccg aacgaatcgg ggcgtggctc
      121 ggcggcgcga cgttcgaagg ctatgagccg ctcgggttca ccgggctgaa gacgtacgac
      181 ccgccgccgg aatcgttggt cggcgcgcgg ttggagcggg tcgaccggcg cgcgaagtac
      241 gtctcgatgt ggttcgacaa cggcctgcgc gtcgtgttcc acctctcgca ggcgggccgg
      301 gtcgacttcg aggaaccgcc gaaacgaacg aagccgaaag gtgcggtcgc gcgctggaga
      361 ttttcggacg gacgtgccgt gttgttgcgc gagcacggca ccgagcgcaa agcgggctgg
      421 tgggtgctgg cgcccggcga cgacggcccg ttggaagggc tcggccccga agcattgagc
      481 gacgaattcg cgacgtggct gcgcacttcg gacgacgggc ggcgtattca cacgatcctg
      541 cgcgaccagc ggacggtcgc gggcgttggc cgcggctatg ccgacgacgt gttgcatcgc
      601 gcgaagttgt cgccgtatgc gtcgctgaag tcgttgtcgc cggacgagcg cgaacggttg
      661 atcgacgcac tgcacgaagt gctggccgag ggactcgaac gcgagcgcag tcgtgagggt
      721 gggttgtcgc agaacaagct cggcgaacat ttcaccgtgc acggcaaagc cggcgtgccc
      781 tgtcccgagt gtggcggcga cttgaaacgc gtgtcgtacg agtcacacga agtcgtgtac
      841 tgcccgagct gtcagacggg cggcaaggtg ctcgccgacc gccgcttgtc gcgcttgatc
      901 aagtgatctg aaggtcggcg taggtttccc agatcaccgc ttcggcgccg tctgcgccgc
      961 ctttcaaggt gcgcgcgccg gcttcggtca tccgcgcggc gtcgccctcg tgcagagtgc
     1021 cgggcccgtc gaggtcggcc tcgccgcgag cgatgaagac gtgcacgaac ggc
//
LOCUS       DASUCJ010001196         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1433884, whole genome shotgun sequence.
ACCESSION   DASUCJ010001196 DASUCJ010000000
VERSION     DASUCJ010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433884"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFU90_13950"
     CDS             complement(<1..499)
                     /locus_tag="VFU90_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5480943.1"
                     /translation="MIEERIFNNRYRLDSKLGEGGMATVYCGTDTVLRRRVAIKVLRE
                     QYAADQEFVRRFYQEAESAARLSHPNIVNTYDVGRENDTYFIVMELVDGASLGEIIAA
                     DGKLPEPVALDYATQIASGLAYAHRTGLLHRDIKPANILVTKDDVVKLSDFGIARAVS
                     EHTMTL"
     gene            complement(496..1902)
                     /locus_tag="VFU90_13955"
     CDS             complement(496..1902)
                     /locus_tag="VFU90_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5480944.1"
                     /translation="MTNRAIRSLSTLFLLLFAVLLARQVYVQAVAAASIAARPQNPRH
                     ALLDAHRGRILATDGSVLAESSGGGRRYPMGAALAQTVGYVSTRYGTSGIEDAFDRAL
                     TPPDTAGDPMAQLAAIRAALHGQTVVSPGADVVTTIVPAVQQRLYDLLSKYPRAAGVA
                     LDPQTGAVLAIASVPSYDPNDFDANFSALTADPHSPLLNRALDGLYPPGSTFKIFTAA
                     AALDNGTVTMDSHFEDPGYLVIGNFTLHDNESEATGYGDLTTAFALSSNVDFAQIALK
                     LGVDGFYQYLDRWGIGDSLDFQLPARTASVPPKDEIVPGELAQMGFGQGALLMTPLQM
                     ALIGATVADGGTEPRPYLVRQVVRNGVAASVYASSALSNPVSAEAAANVTKMMVAVVE
                     RGTGTPARLPHVTVAGKTGTATNPLGRSHAWFVAFAPAENPRVAVAVVVENVGYGAVY
                     AAPIARDVLRAALENAGS"
     gene            complement(1899..>2291)
                     /locus_tag="VFU90_13960"
     CDS             complement(1899..>2291)
                     /locus_tag="VFU90_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480945.1"
                     /translation="DDVADALAHPPAPIVQCAACRRLCVRDEFVWKERQLCAWDYHAQ
                     AFGRRGPWKTGPYEARHFETLPAAAYVAAPLLGELGAEIVLVVGGVEEEAARDAINVL
                     LAADGARPYLAVRTEGGYTLLRETQPPS"
BASE COUNT          373 a          760 c          773 g          385 t
ORIGIN      
        1 tgagcgtcat cgtatgttcc gaaacggcgc gcgcgatccc gaaatcggag agcttgacca
       61 cgtcgtcctt ggtgacgagg atgttggccg gcttgatgtc gcggtgcagt aagcccgtcc
      121 ggtgggcata cgccaagccg ctcgcgattt gggttgcata gtcgagcgcc accggctcgg
      181 gcagtttgcc gtcggcggcg atgatctcgc cgagcgaagc gccgtcgacg agttccatca
      241 cgatgaagta cgtgtcgttc tcgcggccga cgtcgtaggt gttgacgatg ttcggatgtg
      301 acagccgggc cgccgattcg gcttcctgat agaagcggcg gacgaattct tgatcggcgg
      361 cgtattgctc gcgaagtacc ttgatcgcga cgcgccggcg cagcaccgtg tcggtgccgc
      421 agtaaacggt cgccatccca ccttcgccca gcttgctgtc gagccggtag cgattgttga
      481 aaatgcgttc ctcgatcaac tgccggcgtt ctccagggcc gcccgcagca cgtcgcgcgc
      541 gatcggcgcg gcgtacacgg cgccgtagcc gacgttctcg accaccacgg cgacggcgac
      601 gcgcgggttt tcggccggcg caaaggctac gaaccacgcg tgcgagcgcc ccagcggatt
      661 ggtcgcagtg ccggtcttgc ccgcgaccgt cacgtgcggc agtcgcgccg gcgtacccgt
      721 cccgcgctcg actacggcga ccatcatctt ggtgacgttc gcggccgcct cggcggatac
      781 cgggttcgag agggcgctcg acgcgtaaac gctggcggca accccgttgc gcacgacctg
      841 ccgcacgagg tagggacgcg gctcggtgcc gccgtcggcg acggtcgcac cgataagcgc
      901 catctgcagc ggcgtcatca agagcgcgcc ctggccaaac cccatctgcg ccaactctcc
      961 gggtacgatc tcgtccttcg gcgggacgct cgccgtgcgc gcggggagct gaaaatcgag
     1021 cgagtcgccg atgccccaac ggtcgagata ttgatagaat ccgtcgacgc cgagcttcaa
     1081 cgcaatctgg gcaaagtcga cgttgctcga cagcgcaaac gcggtcgtga gatcgccgta
     1141 acccgtagct tcgctttcgt tgtcgtgcaa ggtgaagttt ccgattacca gataccccgg
     1201 atcctcgaag tgcgaatcca tcgtgaccgt tccgttatcc agcgccgccg ccgccgtgaa
     1261 gattttgaag gtcgaaccgg gcgggtagag accgtcgagc gcccgattga gcagcgggct
     1321 gtgcggatcg gccgtcagtg ccgaaaagtt cgcgtcgaaa tcgttcgggt cgtagctcgg
     1381 cacgctggct atcgcgagaa cggcgccggt ctgcggatcg agcgcgacgc cggcggcgcg
     1441 cggatatttg gagagcaaat cgtagagccg ctgctggacc gcgggcacga tcgtcgtgac
     1501 gacgtcggcg cccggtgaga ctacggtctg cccgtgcaac gccgcgcgga tcgccgccag
     1561 ctgcgccatc ggatcgccgg cggtgtcggg cggagtgagc gcccggtcga aggcatcctc
     1621 gatcccgctg gttccgtagc gagtcgaaac gtatccgacc gtttgcgcca aggccgcgcc
     1681 catcggatag cgccgcccgc cgccgctgct ttccgccagc acgctgccgt cggtagccaa
     1741 gatccgcccc cggtgtgcgt cgagcagcgc gtgacgcgga ttctgcgggc gggccgcgat
     1801 cgaggcggcg gcgacggctt gcacgtagac ttgccgggcg agcagtacgg cgaagagcag
     1861 caagaagagc gtcgagaggc tgcgaatcgc gcggttggtc acgaaggcgg ctgcgtctcc
     1921 cgcagcagcg tgtagccgcc ctcggtacgc acggccagat acggccgcgc accatcggct
     1981 gcgagcagga cgttgatcgc gtcgcgggct gcttcttctt ccacgccgcc gaccaccagc
     2041 acgatttcgg cgcccagttc cccgagcaac ggcgcggcca cgtacgcggc cgccggcagc
     2101 gtctcgaaat gccgcgcttc gtagggtccc gttttccagg ggccgcgccg cccgaacgct
     2161 tgcgcgtgat aatcccacgc gcacagctgc cgctctttcc aaacgaactc gtcgcgcacg
     2221 catagccggc ggcacgccgc gcattggacg atcggcgccg gcggatgcgc cagtgcgtcg
     2281 gccacgtcgt c
//
LOCUS       DASUCJ010001197         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_272561, whole genome shotgun sequence.
ACCESSION   DASUCJ010001197 DASUCJ010000000
VERSION     DASUCJ010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272561"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFU90_13965"
     CDS             complement(<1..374)
                     /locus_tag="VFU90_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480946.1"
                     /translation="MSQWLTTIDVTTVRAGELIALNHRIAGAVRATLGVSDTLLPRAD
                     TLNYPEDDGVLPITIELHRDAPATWHADSAADSADARFVRLYDLVLSTMAPNTLRIAW
                     PTDATRDSAEIRVRVRKQVFGGL"
     gene            complement(550..>1292)
                     /locus_tag="VFU90_13970"
     CDS             complement(550..>1292)
                     /locus_tag="VFU90_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5480947.1"
                     /translation="AVVHDTSRAILAQVDLLGWRIVQAVRASLGASGTSVPPGDSFGK
                     WPLPIRHLPFDIVVRHNRRGSWRMGAAADSDAASPALTALYERALGTIPPDSIWMIWP
                     EGYAPDSLVFRLDLVAFGQPGRLMPDKTSLMAVFSATGVAERPALQRQHLYATYPADA
                     RRMRIIGNLLLQFVVDTTGRADTQTIRVLRPTPAQVDSSEYPHYLREFIDAARSAIAR
                     DRFTPARVGRCAVRETVQLPVSYTFRDD"
BASE COUNT          232 a          429 c          431 g          200 t
ORIGIN      
        1 agtccaccga acacctgctt cctcacccgc acgcgaatct cggccgagtc ccgcgtggcg
       61 tcggtcggcc acgcgatacg cagtgtgttg ggcgccatcg tactcaagac cagatcgtag
      121 agacgcacga agcgcgcgtc cgcgctgtcc gcggccgaat cggcgtgcca ggtggccggt
      181 gcatcccggt gcaattcgat ggtgatcggc agcacgccgt cgtcctcggg ataattcaac
      241 gtgtcggcgc gcgggagcag cgtgtccgag acgcctaacg tggcgcgaac ggcgccggcg
      301 attcggtgat tgagcgcgat caactctccc gcgcgcaccg tcgtgacgtc gatcgtcgtg
      361 agccattgac tcatgaaatc gaatgccccg tcccagctgt cgacggaacg gcatgccgcc
      421 gtcgaaccga taccggcttg cgacgaatcg cgcgccgcgg gggactgggg tcgcgccagc
      481 gcgggaagca gtacgactgc cgcgatgatc acgcatgtcc gacgctgctt gttcatagcg
      541 ctccgccgct cagtcatctc ggaaggtgta cgacaccggc aactggaccg tctcgcgcac
      601 ggcacaccgg ccgacgcggg ccggcgtaaa gcgatcacgc gcgatcgcgc tgcgcgccgc
      661 gtcaatgaac tcgcgcaagt agtgcgggta ttcggaggaa tccacctgcg ccggcgtcgg
      721 gcgaagtacg cgtatggtct gcgtgtccgc gcgcccggtg gtatcgacga cgaactgcag
      781 caacaagttc ccgatgattc gcatccggcg ggcatctgca ggataggtcg cgtacaggtg
      841 ctgccgttgg agcgcggggc gctcggcaac accggttgcc gagaacaccg ccatcagcga
      901 ggtcttgtca ggcatcagcc gcccgggttg cccgaaggcc accagatcga gacggaacac
      961 gagcgaatcc ggcgcatatc cctccggcca aatcatccag atcgagtccg gcggaatcgt
     1021 gcccaacgct cgctcgtaga gggcggtgag cgcgggagag gccgcgtcgg aatcagctgc
     1081 tgcgcccatg cgccatgacc cgcgtcgatt gtggcggacg acgatgtcga atggcaggtg
     1141 ccgaatcggg agcggccact tgccgaacga gtcgccgggc ggaacgctgg tcccgctcgc
     1201 accgagcgac gcccgcacgg cttggacgat gcgccacccc aacaggtcca cctgagccag
     1261 gatcgcgcgg ctcgtgtcgt gtaccaccgc ct
//
LOCUS       DASUCJ010001198         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1021391, whole genome shotgun sequence.
ACCESSION   DASUCJ010001198 DASUCJ010000000
VERSION     DASUCJ010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021391"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /gene="infB"
                     /locus_tag="VFU90_13975"
     CDS             complement(<1..>1135)
                     /gene="infB"
                     /locus_tag="VFU90_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5480948.1"
                     /translation="AGGITQHIGAYQIELQGRKITFLDTPGHEAFTQMRARGAQITDI
                     AVLVVAADDGVMPTTLEAIAHARAANVPIVVAINKIDRPDANPDRVKQQLAENGLIAE
                     DYGGDVPMVEVSAREKLGIDDLLEVLLLVADLQELKANPHRAAAGVIIESRIDRNRGV
                     VATVLIQTGTLSLRDIVVAGATYGRVRAMNDDRSRKVRQARPSMPVEILGLLEPPEAG
                     DPLRVVADEREARAIAEERALQRRVGTMTTDRPVRLEELGKQIQSGQARELLIVLKAD
                     VQGSLGALQNALAKVAEEQPQVQLKTLLASTGAITESDVTFAATSDAIVIGFNVRPDA
                     AALRAANATGVDIRFYDVIYTLIDDIEAAMKGLLEPESREVTDG"
BASE COUNT          173 a          396 c          347 g          219 t
ORIGIN      
        1 gccgtcggtg acttcgcggc tctccggttc cagcagaccc ttcatcgccg cctcgatgtc
       61 atcaatcagc gtatagatga catcatagaa gcggatatct actccggtgg cattcgcggc
      121 ccggagcgcg gcggcgtctg gccgcacatt gaaaccgatc acgatagcgt ccgacgtcgc
      181 ggcgaaggtc acgtccgatt ccgtgatcgc gcccgtgctg gcaagcagcg tcttgagctg
      241 cacctgcggc tgttcctcgg caaccttggc gagcgcgttc tgcaacgcgc ccagcgatcc
      301 ttgcacatcg gccttgagca cgatcagcag ttccctggcc tgaccgctct gaatctgctt
      361 acccagctct tccaggcgca ccggccggtc ggtcgtcatc gtgccgaccc ggcgctggag
      421 cgcccgttcc tcggcgatcg cgcgcgcttc gcgctcatcg gcgacgaccc gcagcggatc
      481 accggcttcc ggcggttcga gcaggccgag aatctcgacc ggcatacttg gccgcgcctg
      541 gcggaccttg cgactccggt cgtcgttcat cgcgcgcacg cgaccatagg tcgcaccggc
      601 cacgacgatg tcgcgcagac tgagcgtccc ggtctggatg agcacagtcg ccacaacacc
      661 acgattgcgg tcaatgcggc tttcgataat caccccggcc gccgcgcggt gcgggttggc
      721 cttcagctcc tgcaagtcgg cgacgagcag cagtacttcg agcaggtcgt caatgcccag
      781 cttctcgcgc gccgacacct cgaccatcgg cacatctccg ccgtagtcct cggcgatcag
      841 gccgttttcg gccagttgct gcttgacccg gtcggggttc gcgtccggcc gatcgatctt
      901 attgatggcg acgacgattg ggacgttcgc cgcgcgggcg tgcgcgatcg cttccagcgt
      961 cgtcggcatc acaccgtcgt ccgccgcgac gacgagcacc gcgatatcgg tgatctgcgc
     1021 gccgcgcgcg cgcatctgcg taaacgcttc gtgtcctggg gtatcgagga acgtgatctt
     1081 ccgtccctgc aactcgatct ggtacgcgcc aatgtgctgg gtgatgccgc ccgct
//
LOCUS       DASUCJ010001199         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_975796, whole genome shotgun sequence.
ACCESSION   DASUCJ010001199 DASUCJ010000000
VERSION     DASUCJ010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975796"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFU90_13980"
     CDS             complement(<1..345)
                     /locus_tag="VFU90_13980"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEU5480949.1"
                     /translation="MRVTCVGGGPAGLYFAILAKQRFPDWTVTVLERNRPLDTFGWGV
                     VFSDATLDNLRRADEPTHRAITQAFAHWDDIDIHFEGHTITSGGHGFCGIARKQLLAI
                     LQQRAAEVGVELR"
     gene            complement(361..1020)
                     /locus_tag="VFU90_13985"
     CDS             complement(361..1020)
                     /locus_tag="VFU90_13985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5480950.1"
                     /translation="MTGKSSFIDPAVQEYIIANSVREHPVLAELRIETSQRSNAAMEI
                     GPEQGALMQLLARAIGARRYLEVGVFTGYSSLAMALALPEDGRIVACDVSEEYTAVAR
                     RYWERAGVARKIDLRLAPALETLGNMIAEGVEPFDMAFIDADKANVDAYYEAALKLVR
                     SGGLVLVDNVLWGGLVVNPPQGDDDTAALNALNVKAGRDERVDVALIATCDGILIARK
                     R"
     gene            complement(1017..2546)
                     /locus_tag="VFU90_13990"
     CDS             complement(1017..2546)
                     /locus_tag="VFU90_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5480951.1"
                     /translation="MDSYDAIVVGAGHNGLACAALLGKAGHTVAVFERSPMLGGAAVS
                     DTGVWPGYTLSAASYVCSLLDPWLVDELDLRARGFDFYRKDPYAFTPLADGRSLLLGS
                     DGAANAREIAAFDPGDVAGFRAYGERVDRAGRALFDSFSDAEPRFDRFESDTRALLLG
                     SAAELVERYVDTPVLQAELVNDGLIGTNLGPRDPGTGYVLAHHLAGRVLGVQGAWAFV
                     RGGMGAVSKALADAARSYGVNIRTGATVERILTYDNRAVGVALDDGREIAARAVASNA
                     HPQTTFLELLDATVLEPALRAKVAKWRTAGPSLKVNFALGELPDFTSRPGTQAQPHHR
                     ATIHVAPSIDYLQTAYEDGRTGGESREPLIECFLQTPTDPSLAPPGKHILSAFAQYFP
                     YDRADGWSHAKREAAADKIVAILARFAPNLPNAIEHRQVLAPPDLESRFGLVGGHIFH
                     GELLPGQIYEDRFATRTPVENLYLCGSGAHPGGCVSGFPGKRAAAAITKDHQSATKEV
                     L"
     gene            2572..2757
                     /locus_tag="VFU90_13995"
     CDS             2572..2757
                     /locus_tag="VFU90_13995"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEU5480952.1"
                     /translation="MVSLRVLLAAIPIVALSFAVPLVNRVEPRILGLPFVLFWIVAWV
                     LLTPAFLWTIGRVERRW"
     gene            2754..>3192
                     /locus_tag="VFU90_14000"
     CDS             2754..>3192
                     /locus_tag="VFU90_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5480953.1"
                     /translation="MTPPAVALTIVAVLVLGTIALALFGVRNVRMDPQQFIVGGRSFG
                     TVLLWVLMAGEIYTSFTFLGAAGWAYGFGAPAYYILAYGSCAYVIGYFLLPAIWRVGK
                     ERSLLTSPDFFADRYDSRALGVMAGIIQFGVMVPYVTLQLSGLQ"
BASE COUNT          544 a         1021 c         1021 g          606 t
ORIGIN      
        1 acgaagctct accccgacct cggcggcacg ctgctgaaga atcgcgagga gttgcttgcg
       61 cgcaatcccg cagaacccgt gaccacccga cgtgatcgtg tgcccctcga aatgaatgtc
      121 gatgtcgtcc cagtgagcga aggcttgggt aatcgcccgg tgcgtcggct cgtccgcccg
      181 gcgcagattg tcgagcgtcg catccgaaaa tacgactccc catccgaagg tatccagcgg
      241 acgattgcgc tccagaaccg tgaccgtcca gtccgggaaa cgctgcttgg caaggatggc
      301 gaagtagaga ccggccggtc cgccgccgac gcaggttacg cgcatgctat tgccaagcgg
      361 ctaccgcttt cgcgcgatca agatgccgtc gcacgtcgcg atcagcgcga cgtcgacgcg
      421 ctcgtcccgt ccggccttta cgttgagcgc attcaacgcc gcggtatcgt cgtctccctg
      481 cggcgggttc acgaccaaac ctccccacag gacgttgtcg accaggacga gacccccgga
      541 gcgcacgagt ttgagcgccg cttcgtagta cgcgtcgacg ttggctttgt cggcatcgat
      601 aaacgccata tcgaaaggtt cgacgccttc ggcaatcatg ttccctagcg tctcaagcgc
      661 cggcgccagg cgcaggtcga tcttgcgcgc aacgccggcc cgttcccaat agcgccgcgc
      721 caccgccgta tactcttcgc tcacgtcgca ggcaacgatc cggccgtctt cgggtagagc
      781 aagagccatg gcgagcgaac tgtatcccgt aaaaacgccg acttcgagat atcgccgcgc
      841 gccgatcgcg cgtgccagga gctgcatgag tgcgccttgt tcgggtccga tttccatcgc
      901 ggcgttgctg cgctgtgagg tctcgattcg cagctccgcc aagaccggat gctcgcgcac
      961 gctgttagcg atgatgtatt cctgcacggc aggatcgatg aaggagctct tcccggtcat
     1021 agcacttcct tggttgcaga ctggtggtct ttcgttatgg cggcggcggc gcgcttgccg
     1081 ggaaatccgc tgacgcagcc cccggggtga gctcccgaac cgcaaaggta gaggttctcg
     1141 accggagtcc gggttgcgaa acgatcctcg tagatttgac ccggcagcag ttcgccgtgg
     1201 aagatgtgcc cgcccacgag cccaaagcgg gattcgaggt cgggcggtgc gagcacttgg
     1261 cgatgctcga tcgcatttgg aagattcggc gcaaagcgtg cgaggattgc gacgatcttg
     1321 tcggcggccg cttcgcgttt ggcatgcgac cagccgtcgg cgcggtcgta aggaaagtat
     1381 tgggcgaacg cggagaggat gtgcttgccc ggcggggcca gcgatggatc cgtcggcgtc
     1441 tgcaggaaac attcgatcag cggctcgcgg ctttcgccgc cggtgcgtcc gtcctcgtaa
     1501 gcggtttgca agtaatcgat cgacggcgcg acgtggatgg tcgcgcgatg gtggggttgc
     1561 gcctgggtgc cgggccggga cgtgaagtcc ggtagttcgc cgagcgcgaa gttgaccttc
     1621 agggagggtc cggcggttcg ccacttcgct actttcgccc gcagggcggg ctcgaggacc
     1681 gtcgcgtcca gcaactccaa aaaagtcgtt tgcggatggg cattcgacgc tacggcacgc
     1741 gcggcaattt ctcgcccgtc atcgagcgcc actccgaccg cgcgattgtc gtaggtgagg
     1801 atgcgctcga cggttgcgcc cgtacggatg tttacgccgt agctgcgggc cgcatccgcg
     1861 agggccttcg aaacggcgcc catcccgccc cgcacgaaag cccaggcacc ctgtaccccc
     1921 aggactcgac cggccaaatg gtgcgcgagg acgtacccgg tacccggatc gcgcggcccg
     1981 agattcgttc caatcagccc gtcgttgacc aattccgcct gcagtaccgg cgtatcgacg
     2041 taacgctcga ccagctcggc tgccgatccg agcagcaggg cccgggtgtc ggattcgaac
     2101 cggtcgaaac gcggttcggc gtcggaaaaa ctgtcgaaca gcgcgcgccc cgctcgatcg
     2161 actcgttcgc cgtacgcgcg gaatccagca acgtcgcccg gatcgaacgc cgcaatctcg
     2221 cgcgcgttgg ccgcgccgtc gcttccgagt aagagtgagc ggccgtcggc cagcggcgta
     2281 aacgcatacg ggtccttgcg gtagaaatcg aagcctcgcg cccgcagatc gagttcgtca
     2341 acgagccacg ggtccagaag actgcacacg tacgacgcgg ccgagagcgt atagccgggc
     2401 caaacgccgg tatcgctgac ggccgctcct ccgagcatcg gcgagcgctc gaacaccgcc
     2461 accgtgtggc cagccttgcc gagcagcgcc gcacacgcca agccgttgtg accggcaccg
     2521 acgacgatcg cgtcatagga atccatcacg gggctatact acttaacggc gatggtttca
     2581 ttgcgggtct tactcgcagc cattccgatc gtcgcgctct cctttgcggt gccgttggtc
     2641 aatcgggtcg agccccgaat tctagggctt ccgttcgtcc tgttttggat cgtggcgtgg
     2701 gtcttattga ccccggcctt cctctggacg atcggtcgcg tcgagcgccg ctggtgacgc
     2761 ctccggcggt cgcgctgacc atcgtcgccg tcctcgtgct cggaacgatc gcgctcgcgt
     2821 tgttcggcgt ccgcaacgtt cgcatggatc cgcagcagtt catcgtcgga ggccgctcgt
     2881 tcggaacggt cctgctgtgg gtattgatgg ccggagaaat ctacaccagc tttacgttct
     2941 taggggcggc cggatgggcg tacggattcg gggcgcccgc ctactacata ctcgcctacg
     3001 gtagctgtgc gtacgtgatc ggatactttc tcttaccggc gatctggcgc gtcggaaagg
     3061 aacgctcgct gctgacctct cccgacttct tcgcggatcg ctacgatagc cgggcgctgg
     3121 gcgtcatggc cgggatcatt cagttcgggg tgatggttcc gtacgtgacg ctgcagctca
     3181 gcgggctcca gc
//
LOCUS       DASUCJ010001200         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_333310, whole genome shotgun sequence.
ACCESSION   DASUCJ010001200 DASUCJ010000000
VERSION     DASUCJ010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333310"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFU90_14005"
     CDS             <1..875
                     /locus_tag="VFU90_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5480954.1"
                     /translation="AARPGTLVVGFLNPLSDRTYVDELARAGVTALAMEMIPRITRAQ
                     SMDALSSQSNIAGYKGVLLGAAALPKFFPMLTTAAGTIPPAKVLVLGAGVAGLQAIAT
                     ARRLGAVVSGYDVRAAVKEQVQSLGATFLEFDLGADAEGAGGYAKELTPEQQERQRAW
                     MVEQIGTNDVVVTTALVPGRKAPVLVSEAAVAAMRPGSVIVDLAAEAGGNCALTVADE
                     VVTSPNGVTIVGTVNLPSTMPYHASQLYSRNVHALLSPWLKDGELAVDMNDDVAKGAC
                     VAAGGSALIGGSAS"
     gene            872..1186
                     /locus_tag="VFU90_14010"
     CDS             872..1186
                     /locus_tag="VFU90_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5480955.1"
                     /translation="MTDVSHYISLLTVLVLAVFVGFEVISKVPTTLHTPLMSATNAIH
                     GIVLAGAIVVVAQLFDAGANGPVPTVLAIVAVALGAINVFGGFAVTERMLQMFKRKEG
                     GK"
     gene            1183..2589
                     /locus_tag="VFU90_14015"
     CDS             1183..2589
                     /locus_tag="VFU90_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5480956.1"
                     /translation="MNAASTLAIALADLVAIALFIYGLHELNSPATARRGNRLAMAGM
                     FIALVTVLVTTLGAGWWAIAIGVAIGGTIGVYAALKVKMTAMPQMVALYNGAGGGAAA
                     LISAVEFYVSRGGALRMDDVIAISLVLSAIIGAISFAGSIVAFLKLQELMTGRPVTYP
                     GQQVVNALVALGILALVVWFVTTLGGLPAWGFAALIAAALLLGVLFVLPIGGADMPVV
                     ISLLNSFTGLAVAITGFELSSTLLIICGALVGASGTLLTILMGRAMNRPLTNVLFGAF
                     GAAGGSEPAAGGGGPQQIRTASADDVAIMLAYAGKVIFVPGYGMAVAQAQHSVKALAD
                     QLEKRGVQVAYAIHPVAGRMPGHMNVLLAEANVPYNQLLDMEDVNPEFATTDVALVIG
                     ANDVTNPAARNVPSSPIYGMPILDVDKAANVVVLKRSMRSGFAGIENPLYELPNTSML
                     FGDAKSSVDELTAAVKAL"
     gene            2589..2966
                     /locus_tag="VFU90_14020"
     CDS             2589..2966
                     /locus_tag="VFU90_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5480957.1"
                     /translation="MPGPAERRTIRTGSRWEPLVGYARAVRVGNSVAVSGTVGDGEDA
                     YQQTRSAIATIARALGEAGAALSDVVRTRIFVTDIERDWEQVARAHREAFADVEPACS
                     MVEISRLIAPEFVVEIEADALTP"
     gene            complement(2956..>3569)
                     /locus_tag="VFU90_14025"
     CDS             complement(2956..>3569)
                     /locus_tag="VFU90_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5480958.1"
                     /translation="FLVLVAQYESLTTPLVIMMAVPLALVGAVGGIYLRRLSDLLGIA
                     IHSKIAGHLVLIPQTSSDIYAQIGYVMLIGLAAKNAILIVEFANQLREQGRETVDAVT
                     HAAAERLRPILMTSIAFILGILPLAFAHGDGAQSRISLGTAVLGGMIVSTVMNLAIVP
                     VLYVIVVEWSERRKKKRTAGPPDLPPPPLPEPPPLPEPPPAMV"
BASE COUNT          590 a         1155 c         1215 g          609 t
ORIGIN      
        1 ccgccgcgcg gccgggtacg ctcgtcgtcg gttttctcaa tccgctttcg gaccgcacat
       61 acgtcgacga gctggcgcgc gccggcgtca ccgcgctcgc catggagatg attccgcgaa
      121 tcactcgcgc gcagtcgatg gacgcgctct cgtcgcaaag caacatcgcc ggttacaaag
      181 gcgtgctgct cggcgccgcg gcgctgccga aattctttcc gatgctgacg acggccgccg
      241 gaacgattcc gcccgcaaaa gtcctagtgc tgggcgccgg cgtcgcgggg ctgcaggcga
      301 tcgccacggc gcgccggctc ggcgcggtgg tcagcggcta cgacgtgcgc gccgccgtga
      361 aggaacaggt gcagtcgctc ggcgcgacct ttctcgaatt cgatttgggc gccgacgccg
      421 aaggcgccgg cggctacgcc aaagagctga cgccggaaca gcaggaacgc cagcgcgcgt
      481 ggatggtcga acaaatcggc accaacgacg tcgtcgtgac gaccgctttg gttcccggcc
      541 gcaaggcgcc cgtactggtg agcgaagcgg cggtcgcggc gatgcggccc ggcagcgtga
      601 tcgtcgactt agccgccgaa gccggcggca actgcgccct gaccgtcgcc gacgaagtgg
      661 tgacgtcgcc taacggcgtt acgatcgtcg gaacggtcaa cctgccttcg acgatgccct
      721 accacgcgag ccagctctat tcgcgcaacg tgcatgcgtt gttgtcgccg tggctgaaag
      781 acggcgagct ggccgtcgat atgaacgacg acgtcgccaa aggtgcgtgc gtcgcggccg
      841 gcggcagcgc cttgatcgga ggaagcgctt cgtgaccgac gtttcgcact acatcagcct
      901 gctcaccgtc ttagtactgg cggtgttcgt gggattcgaa gtgatttcga aggttccgac
      961 gacgctgcac acgccgctca tgtcggccac caacgcgatc cacgggatcg tgctggcggg
     1021 ggcgatcgtg gtcgtcgcac agctgttcga tgccggagcc aacggtccgg tcccgaccgt
     1081 gctggcgatc gtcgcggtcg cgctcggagc gatcaacgtg ttcggcggtt ttgcggtcac
     1141 cgagcgcatg ctgcagatgt tcaagcgcaa agagggcggt aaatgaacgc cgcgtcgacc
     1201 ctcgcgatcg cgctggcaga tctggttgcg atcgcgctgt tcatctacgg ccttcacgag
     1261 ctgaactcgc cggccaccgc gcgccgcggc aatcgcctgg cgatggcggg catgttcatt
     1321 gcgctggtaa ccgttctcgt caccacgctc ggcgccggct ggtgggcgat cgcgatcggg
     1381 gtcgcgatcg gcggcacgat cggcgtttac gcggccctca aagtgaagat gacggccatg
     1441 ccgcaaatgg tcgcgctcta caacggcgcg ggcggcggcg cggcggcgtt gatctcggcc
     1501 gtcgagttct acgtctcgcg cggcggcgcg ctccggatgg acgacgtcat cgcgatatcg
     1561 ctcgtgcttt cggcgatcat cggagcgatc agctttgccg gttcgatcgt cgcattcttg
     1621 aaactccagg aactgatgac cgggcggccc gtcacgtatc cgggccagca agtcgtcaac
     1681 gcgctcgtcg cgctcgggat tttggcgctc gtcgtgtggt tcgtgacgac cctcggcggc
     1741 ctgccggcgt ggggattcgc cgcgctgatc gcggcggcgc tgctgctcgg cgtattgttc
     1801 gtgctgccga tcggcggggc cgacatgccg gtcgtgatct cgctgctgaa ctcgtttacc
     1861 ggattggcgg tcgcgatcac cggtttcgag ctgtcgagca cgctgctgat catctgcggc
     1921 gcgctggtcg gggcgagcgg tacgctcttg acgattctga tgggccgggc gatgaaccgt
     1981 ccgctcacca acgtgctgtt cggcgcgttc ggcgcggccg gcggatcgga gccggcggcc
     2041 ggcggcggcg gaccgcagca gatccgcacc gcgagcgccg acgacgtcgc aatcatgctc
     2101 gcgtacgccg gcaaggttat cttcgttccg ggctacggga tggcggtcgc gcaggcgcag
     2161 cacagcgtca aagcgctcgc cgatcagctg gaaaaacgcg gcgtgcaagt cgcctacgcg
     2221 atccatccgg tggccgggcg catgcccggt catatgaacg tgctgctggc cgaagccaac
     2281 gtaccgtaca accagctgct cgacatggaa gacgtcaatc cggagttcgc cactaccgac
     2341 gtcgcgctcg tcatcggcgc caacgacgtc accaatccgg ccgcccggaa cgttccgagc
     2401 tcgccgatct acggcatgcc gatcctcgac gtcgacaaag ccgccaacgt cgtcgtgctc
     2461 aaacgctcga tgcgctccgg cttcgccggc attgaaaacc cgctctacga actgcccaac
     2521 acgtcgatgc tgttcggcga cgccaagtcg tcggtcgacg aactcaccgc cgcggtaaag
     2581 gcgttgtagt gcccggaccc gcggaacgcc ggacgatccg cacgggcagc cgctgggagc
     2641 cgctggtcgg atacgcgcgc gcggtacggg tgggcaattc ggtcgcggtg tcgggaacgg
     2701 tcggcgacgg tgaagacgcc tatcagcaga cgcgctcggc gatcgcgacg atcgcccgag
     2761 cgctcggcga agcgggcgcc gcgttgagcg acgtcgtgcg cacgcgcatt ttcgtaaccg
     2821 acatcgagcg cgattgggag caggtcgcgc gggcacaccg cgaagctttc gcggacgtcg
     2881 agcccgcgtg ttccatggtc gaaatctcgc gtttgatcgc gcccgagttc gtggtcgaaa
     2941 tcgaagccga cgcgctcaca ccatagccgg cggcggttcg ggaagcggcg gcggctcggg
     3001 cagcggcggc ggcggtaagt cgggcggccc cgcggtacgc ttcttcttcc tgcgctcgct
     3061 ccactcgacg acgatcacgt agagcaccgg tacgattgcg aggttcatca ccgtcgagac
     3121 gatcataccg ccgagcaccg ccgttccgag cgagatgcgc gattgcgcgc cgtccccgtg
     3181 cgcgaacgcg agcggcagga tgccgaggat gaaagcgatc gaggtcatca ggatcgggcg
     3241 aagccgctcg gcggccgcgt gcgtgacggc atcgacggtc tcgcgccctt gttcgcgcag
     3301 ctggttggca aactcgacga tcagaatcgc gttcttggcg gccagcccga tgagcatgac
     3361 gtagccgatc tgcgcgtaga tgtcgctcga ggtctgcggt atcaacacca aatgtccggc
     3421 gatcttcgag tgaatggcga ttccgagcag atcggaaagc cgccgaaggt agatcccgcc
     3481 gaccgcgccg accagcgcga gcggaacggc catcatgatc accagcggcg tcgtgaggct
     3541 ttcgtactgc gcgaccagca cgagaaaga
//
LOCUS       DASUCJ010001201         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_150528, whole genome shotgun sequence.
ACCESSION   DASUCJ010001201 DASUCJ010000000
VERSION     DASUCJ010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150528"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFU90_14030"
     CDS             <1..419
                     /locus_tag="VFU90_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5480959.1"
                     /translation="VSRSGTLTYEVVYQLTRAGIGQSTCVGIGGDPINGTSFIDCLEA
                     FEADPDTKAVAMMGEIGGTDEQQAAEFVRAHMSKPVVGFIAGQTAPPGRRMGHAGAII
                     SGSAGTAAEKVEAFKSAGMGVAQRPVDFVPLLKERL"
     gene            416..1324
                     /locus_tag="VFU90_14035"
     CDS             416..1324
                     /locus_tag="VFU90_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5480960.1"
                     /translation="MKLSDLVRVERVVTPLDATTLEAAALVLVERITGAGVVDDPAKL
                     RSRVEEGRGEDIVMLGDRAFLTHYRTDAVLELVVAVGVAASPIPRDAQDVESQQARLI
                     VLIVAPPRQAARYLQVLGAFSRLLSRPDVVEAWLAAQTPAEIAGLAALGEIEITPHLA
                     VRDVMTIRPHTTRPEVVLRDAAREMVRAGVGGLPVVDADGMLVGMLSERELMRHMLVN
                     AAFLGGGRGLHGEAVRHAGGADHTPRTVRDVMTRQVLCVSPDQPLAEVASVMSNKDVE
                     RVPVVRDGRLVGFLTRGDIVRKLIGS"
     gene            1329..1748
                     /gene="ndk"
                     /locus_tag="VFU90_14040"
     CDS             1329..1748
                     /gene="ndk"
                     /locus_tag="VFU90_14040"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5480961.1"
                     /translation="MAGTRTLTIIKPDAFGAGKTGKIIAHLEAAGFKIRAARVLHLTQ
                     AQAQAFYAVHRERPFFAPLVRFMTSGLCMPMVLEHADAVATLRSAIGATDPAEAAAGT
                     VRKLYAESKERNAIHASDSDENAEREAAFFFAEADRI"
BASE COUNT          301 a          605 c          673 g          317 t
ORIGIN      
        1 tcgtgagccg gtcgggcacg ctcacctacg aagtcgtgta ccaactcacg cgcgcgggca
       61 tcgggcagag cacgtgcgtc ggcatcggcg gcgatccgat caacgggacg agtttcatcg
      121 actgcctcga ggcgttcgag gcggatcccg acacgaaagc cgtcgcaatg atgggtgaga
      181 tcggcggcac cgacgagcag caggcggcgg agttcgtgcg tgcgcacatg tccaaacccg
      241 tggtcggatt catcgcggga cagacggcgc cgccgggacg tcgcatggga cacgccggcg
      301 caatcatttc cggctcggca ggaacggcag cggagaaggt ggaggcattc aagtccgcgg
      361 gaatgggcgt ggcccaacgg cccgtggact tcgttccgct gctcaaagaa cggttgtgaa
      421 gctttcggat ctcgtccgcg tcgagcgcgt ggtgactcct ctcgatgcga caacgctcga
      481 ggcggcggcg ttggtgctgg tcgagcgcat caccggcgct ggcgtggtcg acgatccggc
      541 aaagctgcgc agtcgcgtcg aggaagggcg cggcgaagac atcgtgatgc tcggtgatcg
      601 cgcgttcctt acgcactacc gcaccgatgc cgtgctggaa ctcgtcgtcg ccgttggcgt
      661 cgctgcgtcg ccgattccgc gcgacgcgca ggacgtcgag tcgcagcagg cccggctgat
      721 cgtgctcatc gtcgcgccgc cgcgccaggc cgcgcgttat ctccaagtgc tcggcgcgtt
      781 ctcgcggctg ttgtcgcggc ccgacgtggt cgaagcgtgg ctcgccgccc agacgcccgc
      841 ggagatcgcc ggcctcgctg cgttaggcga gatcgaaatc acgccgcacc tcgcggtgcg
      901 tgatgtgatg accatccgcc cgcacaccac gcgtccggaa gtcgtcctgc gcgacgcggc
      961 gcgcgaaatg gtgcgggccg gcgtcggcgg cctgccggta gtcgatgccg atgggatgct
     1021 ggtcgggatg ctgagcgagc gcgagctcat gcggcacatg ctggtcaacg ccgcattcct
     1081 cggcggcggc cgcggcttgc acggcgaagc ggtgcgtcac gccggcggcg cggatcatac
     1141 gccgcgcacg gtgcgcgacg tgatgacccg ccaggtgctc tgcgtgtcgc ccgatcaacc
     1201 gttagccgaa gtcgcttccg tgatgtccaa caaagacgtc gaacgcgtgc ccgtggtgcg
     1261 ggatggccgc ctggtcggct tcctcacgcg cggcgacatc gttcgcaaac tcataggatc
     1321 gtgatgccat ggctgggacg cgtacgctga cgatcatcaa gcctgacgcc ttcggcgcgg
     1381 ggaagacggg aaagatcatt gcgcatttgg aagcggccgg cttcaagatt cgcgcggcgc
     1441 gcgtgctgca tctcacgcaa gcgcaagccc aggcgttcta tgcggtgcat cgcgagcggc
     1501 cgttcttcgc gccgctcgtg cggttcatga cctcagggtt gtgcatgccg atggtgctcg
     1561 agcacgcgga cgccgttgcc acgctgcgct cggcgattgg tgcgaccgac ccggccgagg
     1621 cggcggccgg caccgtgcgc aagctctatg cggaatccaa ggagcggaac gcgatccacg
     1681 catccgactc cgacgagaac gctgagcgcg aagccgcctt ctttttcgcc gaggcagatc
     1741 gcatttagtg gcgcccatcg agtcccgttt ggtaccggtt cagttcgggg cgcccggcgc
     1801 gcggcgagcg ccggaggcga gcgccggaac ggatgcgatg tggtgagctt cctcgctctg
     1861 gagacacgga ttgaacggat gctcgcgcca ctcggg
//
LOCUS       DASUCJ010001202         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_792081, whole genome shotgun sequence.
ACCESSION   DASUCJ010001202 DASUCJ010000000
VERSION     DASUCJ010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFU90_14045"
     CDS             complement(<1..967)
                     /locus_tag="VFU90_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5480962.1"
                     /translation="MVNKRFLPTLIVGLVGAVVGSFLMMLYASSHFGGAAGPDRTPPA
                     VAAVPLSGVSDQDRIVSAVKRTKSSVVAIVEDINGQQYVPADPFLQQFFGQQGQGFER
                     SYRGQASGSGFVIDGQGDIVTNAHVVQPQQGAQVTKMTAVFANGDRIPAHVVAANVGA
                     DVAIIKVDGYGKLPPPLQLADSNRLEQGQWAIAIGEPLELQQTVTVGVVSGFDRSEPI
                     PTENGNVIDFKGLLQTSAPINAGNSGGPLIDIDGQVIGINQSTVKSAYAQGIGFAIPA
                     NTVKSAVADLLKHPGIHQGTDTGFLGIYMSDITSGVRNQIGYNGQG"
     gene            complement(1049..1750)
                     /locus_tag="VFU90_14050"
     CDS             complement(1049..1750)
                     /locus_tag="VFU90_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5480963.1"
                     /translation="MNDAAPAIELRDIHKTYENGPLKVAVLHGITFSVQRGEYLAIMG
                     PSGSGKSTLMNLIGCLDRPTSGTYVLDGIDVSKLDDNALAEIRLRKLGFVFQGFNLLA
                     RTDALKNVALPLFYAGIPVRRRLRRAQEALDEVGIGDRGRHQPSQLSGGQQQRVAIAR
                     ALINDPAVLLADEPTGNLDSRTSDDIMALFGKLHESGRTIIMVTHDENIARHAHRVIR
                     LLDGLVVADDVTQPD"
     gene            complement(1743..>2922)
                     /locus_tag="VFU90_14055"
     CDS             complement(1743..>2922)
                     /locus_tag="VFU90_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5480964.1"
                     /translation="AAGVLMANKLRSLLTIVGLIIGVAAVVAIQVLGNSMAGAIDGLL
                     GGMSDNSFVIFPNPQQRDVTLAAIKLSDLQPIRDAVPEIVDTVPVAGISDLVRNGHHE
                     GRYRISPESAIPFNTLPLLYGRRIDQNDIDDASNVAVLQHTAYSRLFPGGGDPTGQSV
                     YAGPNRFVVIGVLAPPKQGFINASFSGEVAIPWTTYVNRYLRGNSLFAARFVVRDPST
                     IAATEAAVMKTIRTLRGRPDLQYQSFDKSQITQGIDGVFGAMTLVVALIGAVSLLVAG
                     IGIMNIMLVSVAERTREIGVRKAIGARRSQVLLQFFIEALLLCGSGCAIGWAIGVGLG
                     ALVNRFAIVKVTGTVPPLPVLETILIATAFAVVVTLAFGTYPAFRAASLDPIEALRYE
                     "
BASE COUNT          494 a          894 c         1000 g          534 t
ORIGIN      
        1 cgccttgtcc gttgtagccg atttgattgc ggacgccgct ggtgatgtcg ctcatgtaga
       61 tgccgaggaa gccggtgtcg gtgccctggt ggatgccggg gtgcttgagc agatcggcga
      121 ctgccgattt cacggtgttg gccggaatcg caaagccgat gccttgggcg taggccgact
      181 tgacggtcga ttggttgata ccgatcactt ggccgtcgat gtcgatcagc gggccgcccg
      241 agttgccggc gttgatcggt gcggacgttt gcagcaagcc tttgaaatcg atcacgttac
      301 cgttttcggt cgggatcggc tccgagcggt cgaagcccga gacgacgccg acggtcaccg
      361 tctgctgcag ctcgagcggt tcgccgatcg cgatcgccca ctggccctgc tcgagcctgt
      421 tcgagtccgc gagctgaagc ggcggcggca gcttcccgta gccgtccacc ttgatgatcg
      481 cgacgtcggc gccgacgttg gctgcgacga cgtgcgccgg tatccggtcg ccgttggcaa
      541 agacggccgt catcttggtg acctgcgcgc cctgctgcgg ctgaacgacg tgcgcgttgg
      601 tgacgatgtc gccctggccg tcgatcacga agcccgagcc cgaggcctgc ccgcgatagg
      661 agcgctcgaa gccttggccc tgctggccga agaactgctg caggaacggg tcggctggga
      721 cgtactgttg gccgttgatg tcttcgacga tcgcgaccac cgacgatttc gtccgcttga
      781 cggcactgac gatgcggtcc tggtcgctca ccccgctcag gggaacggcg gcgacggcag
      841 gcggggtccg gtcgggaccg gccgctccgc cgaagtgcga gctggcatag agcatcataa
      901 ggaagctgcc gacgacggcg cctacgagac cgacgatgag ggtgggaagg aagcgtttat
      961 tcaccattct ccaaaccatg ttgctgcgcg agtgacttaa agctttctac catcctaacg
     1021 ccaaaaggtc gcagattgtg gcaaaaaact agtcgggctg ggtgacgtcg tccgcgacga
     1081 ccaggccgtc gagcaagcgg atcacccggt gggcatggcg agcgatgttc tcgtcgtggg
     1141 tcaccataat gatggtacgc ccgctttcat ggagtttgcc gaaaagcgcc atgatgtcgt
     1201 cgctggtgcg ggaatcgagg ttgccggtcg gctcgtcggc cagcagcacg gccggatcgt
     1261 tgatcaaggc ccgggcgatc gcgacgcgct gctgctgccc gcccgagagc tggctgggct
     1321 ggtggcgccc ccggtcgccg atcccgacct cgtcgagggc ttcctgggcg cgccgcagcc
     1381 ggcggcgcac cgggatgccg gcgtagaaga gcggcagggc gacgttcttg agcgcgtcgg
     1441 tgcgtgccag cagattgaac ccttggaaga cgaagccgag tttgcgcaag cgaatttcgg
     1501 ccagtgcgtt gtcgtcgagc ttggaaacgt caatgccgtc gagcacgtag gtgccgctgg
     1561 tggggcgatc gagacagccg atcaggttca tcagggtgga tttgcccgaa cccgacgggc
     1621 ccatgatcgc gagatactcg ccgcgctgta ccgagaacgt gatcccgtgc agcacggcaa
     1681 ccttgagcgg cccgttttcg taggtcttgt ggatgtcgcg cagttcgatc gccggcgccg
     1741 cgtcattcat agcgcagcgc ctcgatgggg tcgaggctgg ccgcacggaa cgccggatag
     1801 gttccgaacg ccagcgtcac gaccaccgcg aacgccgtcg cgataagaat ggtttcgagt
     1861 accggaagcg gcggaacggt gccggtgact ttcacgatcg cgaagcggtt gaccagcgcg
     1921 ccgagcccga cgccgatcgc ccagccgatc gcgcacccgc tgccgcacag cagcagcgcc
     1981 tcgatgaaga actgcaggag cacttgcgac cgccgggcgc cgatcgcttt gcgcacgccg
     2041 atttcccgcg tccgctcggc gaccgagacg agcatgatgt tcatgatgcc gatgcctgcg
     2101 accagcagcg aaacggcacc gatcagcgcg accaccagcg tcatcgcgcc gaacacgccg
     2161 tcgatccctt gcgtgatctg cgatttgtcg aagctctgat attgcagatc gggccggccg
     2221 cgcagcgtgc ggatcgtttt catcacggcc gcctcggtgg cggcgatcgt cgatgggtcg
     2281 cgcacgacga atcgtgccgc gaacagcgag ttcccgcgca gataacggtt gacgtacgtc
     2341 gtccacggga tcgcgacctc gccgctgaag ctggcgttga tgaagccttg tttcggcggc
     2401 gccagtactc cgatcacgac gaaccggttg gggccggcgt agacgctttg cccggtcggg
     2461 tcgccgccgc ccgggaagag ccgcgagtag gcggtgtgct ggagtacggc gacgttggag
     2521 gcgtcgtcga tatcgttctg atcgatccgg cggccgtaga gcagcggcag cgtgttgaac
     2581 gggatcgcgc tttcgggcga gatgcgatag cggccctcgt gatggccgtt gcgcacgagg
     2641 tcgctgattc cggccaccgg cacggtgtcg acgatctccg gtacggcgtc gcgaatcggc
     2701 tgcaaatcgc tcagcttgat cgcggccagc gtgacgtcgc gttgttgcgg attgggaaag
     2761 atgacgaacg agttgtcgct catcccgccg agcaatccgt cgatcgcgcc ggccatgctg
     2821 tttccgagca cttgaattgc gaccaccgcg gcgacgccga tgatcaagcc gacgatcgtc
     2881 agcagcgatc gcagcttgtt ggccatcagc acgccggcgg ct
//
LOCUS       DASUCJ010001203         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_960139, whole genome shotgun sequence.
ACCESSION   DASUCJ010001203 DASUCJ010000000
VERSION     DASUCJ010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960139"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /gene="gcvPB"
                     /locus_tag="VFU90_14060"
     CDS             complement(<1..>1088)
                     /gene="gcvPB"
                     /locus_tag="VFU90_14060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU5480965.1"
                     /translation="LAQGALQVMYELERSLSSLFGMAAFSLNPAAGAHAELAALLIAK
                     RYFRDRGETGRDKVIVPDTAHGTNPASAAMCGFKVISLPSDERGRVSLDELAKVVGAD
                     TAVCMMTNPNTLGLFEDRVAEITAAVHAAGGLMYYDGANANALMGNARPGDMGFDLMH
                     LNTHKTFTIPHGGGGAGHGPVGVAAHLVDYLPTPVVVATPNTNGDSTAAPSDRMTFRL
                     DFDRPKSIGPMRSFWSNFAHAVRALAYIYANGAEGLKRVSQLAVLNANYLRVKVAEFL
                     ETPYDEICRHEFVVSAQALKRETGVRALDIAKALLDRGFMAPTMYFPLIVPECLMIEP
                     TETESKETLDTFAAALREIVAEAKSNPE"
BASE COUNT          184 a          347 c          361 g          196 t
ORIGIN      
        1 cctcggggtt cgatttagcc tcggcgacga tctcgcgcaa cgccgcggcg aacgtatcga
       61 gcgtctcctt cgactcggtt tcggtgggct cgatcatcaa gcactcgggg acgatcagcg
      121 gaaagtacat cgtcggcgcc atgaacccgc ggtcgagcag cgccttggcg atatccaagg
      181 cgcgaacgcc ggtttcgcgt ttgagcgcct gcgccgagac gacgaactcg tggcggcaaa
      241 tttcgtcgta gggcgtctcc aagaactccg cgaccttcac gcgcaggtag ttggcgttga
      301 gcacggcgag ctgcgaaacc cgcttgagcc cctcggcgcc gttggcgtag atatacgcca
      361 gcgcgcggac cgcgtgtgcg aagttcgacc aaaacgagcg catcggcccg atcgacttcg
      421 gacggtcgaa atcgagccgg aaggtcatcc ggtcgcttgg ggccgccgtc gagtcgccgt
      481 tcgtgttcgg cgtcgctacg accaccggcg tgggcagata gtcgaccaag tgcgctgcga
      541 cgccgaccgg accgtgcccc gcgccgccgc cgccgtgcgg aatggtgaac gtcttgtgcg
      601 tgttgaggtg catgagatcg aagcccatat cgccgggacg cgcgttgccc atcagcgcgt
      661 tggcgttggc gccgtcgtag tacatcaggc cgcccgcggc atgcactgct gcagtgatct
      721 ccgcgacgcg atcctcgaat agtcccagcg tgttgggatt ggtcatcatg caaacggcgg
      781 tgtctgcgcc gacaactttt gccagttcgt cgaggctgac gcggccgcgc tcgtcgctcg
      841 gcagcgaaat cactttgaat ccgcacattg ccgccgaggc cggattggta ccgtgggcgg
      901 tatccggaac gatcactttg tcgcggccgg tttcgccgcg atcccggaaa tagcgtttgg
      961 cgatcagcag cgcggcgagc tcggcgtgcg cgccggccgc cgggttcagc gagaaggccg
     1021 ccatgccgaa cagggagctc aagctgcgct ccagctcgta catcacctgc agcgcaccct
     1081 gagcgagt
//
LOCUS       DASUCJ010001204         1451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1281441, whole genome shotgun sequence.
ACCESSION   DASUCJ010001204 DASUCJ010000000
VERSION     DASUCJ010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281441"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFU90_14065"
     CDS             complement(<1..452)
                     /locus_tag="VFU90_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480966.1"
                     /translation="MSIQPLEGSLGSTPSTPIAAFGEGASSLAAGLAPSALPGDAISR
                     MVPPWLIGSMSNPGETAAFGPLPGLMQQLLQMLQQLMGMLGGTPYAGSGNCLPYPGQG
                     TGCAPYGNERFFADATGASQGDPHLSFNGAKWNSMASQPNLLESNSIPG"
     gene            644..>1451
                     /locus_tag="VFU90_14070"
     CDS             644..>1451
                     /locus_tag="VFU90_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU5480967.1"
                     /translation="MELEERSRAEGFWNDPDAAQRVMKESAELRAETEAIERVERTLG
                     DARELLELFSGDPAGQSEAEHGVAGAAQEIEKLEMAAAFSGEFDAHNAIVSIFAGAGG
                     VDAADWTAMLARMYLRWAEAKGYSTQVVDESAAEEAGLKSVTFFVRGRNAYGMLESER
                     GVHRLVRLSPFDAAHRRHTSFAAVDVIPEIEAGEKFDVEIKPEDLRVETFKSGGAGGQ
                     YVNKTESAVRVIHLPSGIIVASQQERSQAQNREVAIGILRAKLAQRAAEER"
BASE COUNT          281 a          461 c          487 g          222 t
ORIGIN      
        1 cccggaatgg aattcgactc cagcaaattc ggctgcgaag ccatcgaatt ccacttggcg
       61 ccgttgaagg aaaggtgcgg atcgccttgg ctcgcgccgg tcgcgtcggc aaagaagcgt
      121 tcgtttccgt agggagcgca gccggtgcct tggccgggat acggaagaca gttgccggat
      181 cccgcgtacg gcgtaccgcc gagcatcccc atcaactgct gcagcatttg caggagctgc
      241 tgcatcaacc cggggagcgg tccgaacgcc gccgtttctc cgggattcga catcgatccg
      301 atcagccacg gtggaaccat ccgcgaaatc gcgtcgccgg gcagcgcact cggtgcgagc
      361 cccgctgcga gcgagctcgc gccttcgccg aatgcggcga tgggagtgga aggggtcgat
      421 ccgagcgacc cttcgagcgg ctggatggac atgaatcaac ctccgtcgtt atggtgcaac
      481 ggcgttcacg acggatgcgc gcgccgacaa ggggccggcg acgatttgtc agaagcgcgt
      541 tgcgccatga gcgacacgct gcaaaccacc atcgcccgcc tccgcgagcg cctcgccgcg
      601 ctcaaggagc gtctttgact atgccggcaa gactcggcgc gtaatcgagc tcgaagaacg
      661 ctcgcgtgcg gaaggatttt ggaacgatcc cgacgccgcg cagcgcgtga tgaaagagtc
      721 ggcggaactg cgtgccgaga ccgaagcgat cgagcgggtc gagcgaacgc tcggcgacgc
      781 acgcgaactg ctggagctgt tctcgggcga tccggccggc cagagcgaag ccgagcacgg
      841 cgtcgccggc gccgcccaag aaatcgagaa gctcgaaatg gcggccgcct tcagcggcga
      901 gttcgacgcc cacaacgcga tcgtcagcat ctttgccggc gccggcggag tcgatgcggc
      961 cgactggacc gcgatgctcg cgcggatgta tcttcgctgg gccgaagcca aggggtattc
     1021 gacccaagtc gtcgacgaat cggccgccga agaagccgga ctgaaatcgg tgacgttttt
     1081 cgtccgcggc cgtaacgcgt acggcatgct cgaaagcgag cgcggcgtcc accggctggt
     1141 gcgcctctcg ccgttcgatg cggcacatcg ccggcacacg tcgtttgcgg cggtcgacgt
     1201 catcccggaa atcgaagccg gcgagaagtt cgacgtcgaa atcaagcccg aagatctgcg
     1261 ggtcgaaacc ttcaagtcgg gcggcgccgg cggacagtac gtcaacaaaa ccgagtcggc
     1321 ggttcgggtg attcaccttc cgagcggcat catcgtcgcg tcgcagcaag agcgatcgca
     1381 ggcgcagaac cgcgaggtcg cgatcggcat tctccgcgcc aagctggccc agcgcgccgc
     1441 cgaggaacgc g
//
LOCUS       DASUCJ010001205         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1158621, whole genome shotgun sequence.
ACCESSION   DASUCJ010001205 DASUCJ010000000
VERSION     DASUCJ010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158621"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFU90_14075"
     CDS             complement(<1..1173)
                     /locus_tag="VFU90_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480968.1"
                     /translation="MTAVPNRDFGIWQRIGGVLAYLPPTVFLIVAFLLVFVRPSVMTW
                     SFYVFAVGYFGTSPAFAYWAHVLPPAAFFVLTFVLSTVFGAWSVLPLLPFVLRFPNGD
                     VLGWRRRIDPFVWLFLAVTFAGAVAEWRYYGANAALPSWDVILSTVVPLATFAFAAAI
                     VAKNIAVATPRDRQRWGFLAIGTIASFLAYAIYFVPGVPFAVGQAIGFAVVLMPICIG
                     YAVFRLRVIDVNFVLNRALVYGSLSLLVVAFVSLLDWTLSRVVSEERLALGLELLATI
                     AIGFLLDKINRVVERFVETIFFRRRREAESYLRRAAAALPYATDEAAIADGLVQVPVD
                     ALDLAAAAIYRRADGGARFEGVATSHQTPIAPPGFDANHLLVRMLLAGEKKVWLDEV"
BASE COUNT          271 a          456 c          350 g          140 t
ORIGIN      
        1 cacttcgtcg agccaaacct tcttctcgcc ggccagcagc atccgcacca gcaaatggtt
       61 ggcgtcgaac cccggcggcg cgatcggcgt ctgatgcgac gtcgcgacgc cctcgaaacg
      121 cgccccgccg tccgcgcggc gataaattgc cgccgccgct aagtcgagcg cgtcgaccgg
      181 cacctgcacc agcccgtcgg cgatcgccgc ttcgtcggtc gcgtacggca gcgccgcggc
      241 cgcccgccgc agataacttt cggcctcgcg ccgccgccgg aagaaaatcg tttcgacgaa
      301 gcgctcgacc acgcgattga tcttatcgag cagaaagccg atcgcgatcg tcgccagcag
      361 ctccaatccc agcgccagcc gttcttcgga aacgacccgc gacagcgtcc agtcgagcag
      421 cgacacgaac gccaccacca gcagcgacag cgacccgtag accagcgcgc ggttgagcac
      481 gaagttcacg tcgatcacgc gcagccggaa gaccgcgtac ccgatgcaga tcggcatcag
      541 caccaccgcg aagccgatcg cttgcccgac cgcaaacggc acgcccggca cgaagtaaat
      601 cgcgtacgcc aggaacgacg cgatcgtgcc gatcgccaaa aatccccacc gctgccgatc
      661 gcgcggcgtc gcgaccgcga tattcttggc gacgatcgcc gccgcaaacg caaaggtcgc
      721 cagcggcacg accgtgctca agatcacgtc ccacgacggc agcgccgcgt tcgcgccgta
      781 gtagcgccac tccgccaccg cccccgcaaa ggtgacggct aagaacagcc acacgaacgg
      841 atcgatccgc cgccgccagc cgagcacgtc gccgttggga aagcgcagca cgaacggcag
      901 cagcggcagc acgctccacg caccgaagac cgtgctcaac acgaacgtca gcacgaagaa
      961 cgccgccggc ggcagcacgt gcgcccagta cgcgaacgcc ggactcgtcc cgaaatagcc
     1021 gaccgcaaac acgtagaacg accacgtcat caccgacggc cgcacgaaca ccagcaagaa
     1081 tgcgacgatc agaaacaccg tcggcggcaa ataggcgagc acgccgccga tccgttgcca
     1141 gatgccgaaa tcgcgattcg gcacggccgt catcgtgacg gtaaacgtcg acccgccgcg
     1201 tacgaacgtc agcgtgt
//
LOCUS       DASUCJ010001206         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_12995, whole genome shotgun sequence.
ACCESSION   DASUCJ010001206 DASUCJ010000000
VERSION     DASUCJ010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12995"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFU90_14080"
     CDS             <1..479
                     /locus_tag="VFU90_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEU5480969.1"
                     /translation="KINVYDLSVDGSLGGYANGIRIKSDSSRGGLVNDVTYSDVCVRG
                     LETPILLTPFYTTNTGSDIPQFTNIKIQNFHALNGPSNQKVTFDGYDASHINTVTLDN
                     VVVDGITAKQVEASYTTFTLGPGNVNFMPTGTSVNVTNNISGSSTPNPCTGKWVTF"
     gene            complement(469..780)
                     /locus_tag="VFU90_14085"
     CDS             complement(469..780)
                     /locus_tag="VFU90_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480970.1"
                     /translation="MAPPLAFPLVPAPLAPAPPVAPVAEPLPPATPVLALSEAPAIEA
                     PAAPVAAPLAAPEASPVAAPPAAGDSLPESPEPHAASVQAATNTGARRPSSSAVRDLK
                     T"
BASE COUNT          178 a          338 c          356 g          145 t
ORIGIN      
        1 cgaagatcaa cgtctacgat ctttccgtcg acggatcgct cgggggctat gcgaacggaa
       61 ttcgcatcaa gtcggactcg agccgcggcg gcctcgtgaa cgacgtcacg tacagcgacg
      121 tctgcgtgcg cggcctcgag acgcccatcc tcctcacgcc gttctatacg acgaatacag
      181 ggagcgacat tccgcaattc acgaacatca agatccagaa ttttcacgcc ttgaacgggc
      241 cgagcaatca aaaggtgacg ttcgacggat acgacgcgtc ccacatcaat acggtcacgc
      301 tcgacaacgt cgtcgtcgac gggatcacgg cgaagcaagt cgaggcaagc tacaccacgt
      361 tcaccctcgg gcccggcaac gtgaacttca tgcccacggg tacgagcgtc aacgtcacga
      421 acaacatttc cggatcgagc acgcccaatc cgtgcaccgg caaatgggtc acgttttgag
      481 gtcgcgtacc gcgctcgagc tcggcctccg cgcgcccgta ttcgtcgccg cttgcacgct
      541 ggccgcgtgc ggctcgggag attcggggag cgagtcgccg gcggccggcg gtgcggcgac
      601 gggcgatgcc tctggcgcgg cgagtggcgc agctaccggt gcggcgggtg cttcgatggc
      661 aggcgcttcc gacagcgcga gcaccggcgt cgcgggcggt agcggctcgg cgaccggcgc
      721 cacgggcggg gccggcgcga gcggcgcggg aacgagcgga aacgcgagcg gcggcgccac
      781 cggatcgagc gcggggtcgg accgcgacgc gggggcaaag gccgacgcga ctgtaccgcg
      841 cgatgcgggc gggacgcgcg atgcgtccgt ggacgctagc ggcggccggc cgagcgacgc
      901 cggcaccggc gaaggtgcgc tggcttccgg cgtgatggcg ttccctgcgc caggcgcaaa
      961 aggcgtctgt ccggatccgc ccctgcgact cacgttctcc gggccgccca ccttggg
//
LOCUS       DASUCJ010001207         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_807710, whole genome shotgun sequence.
ACCESSION   DASUCJ010001207 DASUCJ010000000
VERSION     DASUCJ010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807710"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..>1475)
                     /locus_tag="VFU90_14090"
     CDS             complement(104..>1475)
                     /locus_tag="VFU90_14090"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5480971.1"
                     /translation="IHSHADDGLFVDPLAESIIRQGVTTVIVGQDGSSRAPTRTGEAG
                     EPAYATMRGFFAAIDALPSAVNVASMLGLGTVRHVVVGDDDRPATRDELRRMTALVEA
                     ALADGACGVSSGLEYIPGQFAPLGELIALSRPCARRRLPYATHMRNEDDRLLEALDEA
                     IAVAGGAGCPLEVSHLKTEGRRNWNKIDEVLRRIHGARVSGLDVTFDRYPYIAYQTDL
                     GNLFPAWSKDGGNDAFVGRLSQPDLAPKLRAAAAEKIRLLGGWDNVLISSVAADDDRK
                     VEGQRMGQYASSIGRDPFDAAVGLLVRSHGDVGMVGFAMSETNLERFLADPLCTICSD
                     GGAVAISGPGRSGHPHPRSLGTFARILGHYVRERHVLTLPQAIHKMSAQTAARMRLPN
                     RGRIAPGYAADVVVFDPSTVSDRATFADPFQYAVGVSAVVVNGGIALRDGQRGPRTGR
                     SIALRA"
BASE COUNT          240 a          517 c          474 g          244 t
ORIGIN      
        1 tccgtgcttt gtccgcgtct tcatgagcgt agagaccttc cagccgcctc acacaggcca
       61 accatctata gtataggact ggcccctcca aacgccggct cggctacgcc cggagcgcta
      121 tcgaccgacc cgtcctcggc ccgcgttgtc cgtcgcgaag cgctattccg ccattcacga
      181 ccaccgctga gaccccgact gcgtactgaa acggatcagc gaacgttgcc cggtcgctca
      241 ccgtcgatgg atcgaacacc acgacgtcgg ctgcgtatcc gggcgcgatt cgtccgcgat
      301 tcggcagtcg catgcgggcc gctgtttgcg ccgacatttt gtgaatcgcc tgcggcaggg
      361 tgagcacgtg gcgctcgcgc acgtagtggc cgaggatgcg tgcgaacgtt ccgagcgagc
      421 gcgggtgcgg gtgaccggaa cgacccgggc cggaaatcgc gactgcgccg ccgtcactgc
      481 aaatcgtgca caacggatcg gcgagaaagc gttcgagatt cgtctcgctc atggcgaaac
      541 ccaccatgcc cacgtcgcca tggcttcgca cgagcagccc caccgccgcg tcgaacggat
      601 cccgtccgat cgaagacgcg tactggccca tgcgctgtcc ttccaccttg cgatcgtcgt
      661 cggccgcgac ggacgagatg agcacgttgt cccatccgcc taacaatcga atcttctcgg
      721 ccgccgccgc ccgcagcttg ggcgcgagat ccggctgcga cagccgccca acgaaggcat
      781 cgttgccgcc gtccttgctc caggccggga acaggttgcc caagtcggtc tgatacgcga
      841 tgtacggata gcgatcgaac gtgacgtcga gacccgacac gcgcgcgccg tggatgcgcc
      901 ggagcacttc gtcgatcttg ttccaattgc gccgcccttc cgtcttgagg tgcgacactt
      961 cgagcgggca gcccgcgccg cccgccacgg cgatcgcttc gtccagcgcc tcgagcaatc
     1021 ggtcgtcttc gttgcgcatg tgcgtggcgt acggcagccg gcgccgcgcg cacgggcgcg
     1081 agagggcgat cagctcgcct aacggagcaa attgtcccgg gatgtactcg aggcccgacg
     1141 acacgccgca cgcgccgtcg gcgagcgcgg cctcgacgag cgcggtcatg cgacgcagct
     1201 cgtcgcgtgt cgcgggccgg tcgtcgtcgc ccacgaccac gtgccggacg gttccgagcc
     1261 cgagcatgct cgccacgttc acggcgctcg gcagtgcgtc gatagcggca aagaagccgc
     1321 gcatcgtggc gtacgcgggt tcgcccgctt cgccggtccg cgtgggcgcg cgcgatgagc
     1381 catcctgccc aacgatgacc gtcgtcacgc cctgccggat gatggattcg gcgagcgggt
     1441 ccacgaacag cccgtcgtcg gcgtgggagt ggatg
//
LOCUS       DASUCJ010001208         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1464828, whole genome shotgun sequence.
ACCESSION   DASUCJ010001208 DASUCJ010000000
VERSION     DASUCJ010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464828"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFU90_14095"
     CDS             <1..196
                     /locus_tag="VFU90_14095"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5480972.1"
                     /translation="FRRDEHMRPDTTLEGLAKLKPVFKADGVVTAGNASGIGDGAGAL
                     VVSSADWAAKNGKKPLARLV"
     gene            203..547
                     /locus_tag="VFU90_14100"
     CDS             203..547
                     /locus_tag="VFU90_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480973.1"
                     /translation="MAGVDPTIMGIGPVPATRIALRKAGMSLDDMDLIEVNEAFAPQV
                     CAVERELEIPREKFNVHGGAIALSHPLAASGARITAHLIHALRAGGKRFGLGSACIGG
                     GQGIAVVIEAFV"
     gene            597..1733
                     /locus_tag="VFU90_14105"
     CDS             597..1733
                     /locus_tag="VFU90_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480974.1"
                     /translation="MSRISASSIARAVVLWSLTAVAVACASSSNGPQGTAEPLAARPD
                     LTWRISTRYQVDLWLHGYALLQKDTTLIPYFKPGYRDSIIAFKKRGNITTVLDTDKDS
                     LETQLNSDSKLVGGQFLGLYFRNWESTQKAIDSYLKSSSSEESARRMNVRQMVDPDQR
                     AVYGIIQASYSTPAERQWLRKFSAAMQDENTKYYANWWRTQQSQRAAIFAAVDSLWAQ
                     TYFAKFRPFLTKSGQSRGEILLSLPLDGEGRTLGEPGRGPTITITFPDSVSRAVDAMY
                     VLAHEAVSPVSNAAVNDNTTPAEKKEGVADRYTSPAAVRGGLQLLQKIAPELAIGYAR
                     YYLDAAHISYTPGAEVAAIEKAFPLRQTIAEQLTNQLNTDTSGS"
     gene            1795..2544
                     /locus_tag="VFU90_14110"
     CDS             1795..2544
                     /locus_tag="VFU90_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480975.1"
                     /translation="MERVLARAAELQAADADPTDTALNEDQLIEVAREVGLSPEHLKQ
                     ALAEERSRAQVPDESGLTARLFGAAHVHASRTIKGTAATTLDRLDMWLQREESMQVKR
                     RLPDRMLWEPRPGLFSEMRRLLNVSGRGYYLRPTREVGATVVPVDADRVLVRLEADLT
                     NVRTERAASGAVLAGGGVLGTGALIALGFFVPVAIIPAAAAAVGGYFVARSHAPLVAR
                     AQLALEQLLDRLERGDSPPRPNVISALDVWR"
BASE COUNT          481 a          864 c          868 g          437 t
ORIGIN      
        1 gttccgtcgc gacgagcaca tgcggccgga cacgacgctc gaagggctcg ccaaattgaa
       61 accagtgttc aaggccgacg gcgtcgtgac ggccggcaat gcgtcgggca tcggcgacgg
      121 tgcgggtgca ctcgtcgtga gcagcgcgga ttgggcagcg aagaacggca agaagccgct
      181 cgcgcgcctc gtgtaatggg gtgtggcggg tgtcgatccg accatcatgg gcatcggacc
      241 tgttcccgcc acgcgcatcg cgctccgtaa ggcgggcatg tcgctcgacg acatggactt
      301 gatcgaagtg aacgaagcgt tcgcgccaca ggtgtgcgcc gtcgagcgtg agctggagat
      361 cccgcgcgag aagttcaacg tgcatggtgg ggccatcgcg ctgagccatc cgttggccgc
      421 gtcgggcgcg cgaatcacgg cgcacctgat tcacgcgttg cgggccggcg gcaagcgctt
      481 cggactcggt tccgcgtgca tcggcggcgg acagggaatc gctgtggtca tcgaggcctt
      541 tgtatgatgc gcgctccaat ggcgcgtcat gccactctag caccggatcc attcccgtga
      601 gccgaatctc agcgtcgtcc atcgcccgtg ccgtggtgct gtggtcgttg acggccgtcg
      661 ccgttgcatg cgcttcgtcc tcgaacggtc ctcagggcac cgctgaaccg cttgcggcgc
      721 gtcccgatct gacgtggcgg atcagcaccc gctaccaagt cgacctctgg ctgcacggtt
      781 atgcgctgct gcagaaagac acgacgctca tcccgtactt caaaccgggg taccgcgatt
      841 cgatcatcgc gttcaagaag cgcggcaaca tcaccacggt gctcgacacc gacaaggatt
      901 cgctcgagac gcagctcaac tccgactcga agttggtcgg cggacagttc cttggacttt
      961 atttccggaa ctgggaaagc acgcagaagg cgatcgactc gtacttgaag agctcgtcca
     1021 gcgaggaatc ggcgcgccgc atgaacgtgc ggcagatggt cgaccctgat cagcgggccg
     1081 tgtacggaat cattcaagcg tcctactcga ccccggccga gcgtcaatgg ttgcgcaagt
     1141 tctcggcggc gatgcaggac gagaacacca agtattacgc caactggtgg aggacgcagc
     1201 aatcgcagcg ggccgccatc ttcgcggcgg tcgattcact ctgggcgcaa acgtacttcg
     1261 ccaagttcag accgttcctc accaagtcgg gacagtcgcg cggcgagatc ctgttgtcgc
     1321 tgccgctcga cggcgaaggc cgcacgttag gcgaacccgg tcgcggccca acgatcacga
     1381 tcacgttccc cgacagcgtc agccgcgccg tcgatgcgat gtacgtgctc gcgcacgaag
     1441 ccgtctcgcc ggtgtcgaac gctgccgtaa acgacaacac gacgcccgcc gaaaagaaag
     1501 aaggcgtggc cgaccgatac accagtccgg cggcggtgcg cggcgggctg caactcttgc
     1561 agaagatcgc gcccgagctg gcgatcgggt acgcgcgtta ttacctcgac gcggcgcaca
     1621 tctcctacac gcccggcgcc gaggtcgcgg ccatcgaaaa agcatttccg cttcggcaga
     1681 ccatcgccga gcagctcacc aaccaactca acacggatac gagcggcagc tgagtgatgc
     1741 atggtggatg agagcgggct gcccgcacga cgtcagccgc tcgatcgcgc ggcgatggag
     1801 cgggtcctgg cgcgcgcagc cgaattgcag gcggccgacg ccgatccgac ggacactgcg
     1861 ctcaacgaag atcagctgat cgaagtcgcg cgcgaggtcg gtctctcacc cgagcatctc
     1921 aagcaggcgc tcgccgaaga gcgaagtcgc gcgcaagttc cagacgagag cggcttgaca
     1981 gcgcgactgt tcggtgccgc tcacgtccat gcatcgcgca cgatcaaagg gaccgcggcg
     2041 acgacgctgg accgactgga catgtggttg caacgcgaag agtctatgca ggtcaagcgt
     2101 cgtctcccag atcgcatgct ctgggagcca cgccccggcc tgttcagcga gatgcgacgg
     2161 ctgctcaacg tgagcgggcg cggctactac ttgcggccga cgcgcgaagt cggggcgacc
     2221 gtcgtgcccg tcgacgccga tcgcgtcctc gtgcggctcg aggccgacct gaccaacgtg
     2281 cggacagagc gcgcggcctc cggcgccgtg ctcgcgggcg gcggcgtgtt gggcacgggc
     2341 gcgctcatcg cactcggatt tttcgtgccg gtcgcgatca ttcccgcggc ggcggccgcc
     2401 gtgggcggat acttcgtcgc ccggagccac gcgccgctcg ttgcgcgcgc ccaactggcg
     2461 ctggaacaat tgctcgaccg tttggagcgc ggggacagtc ccccgcgtcc gaacgtgata
     2521 tcggcgctcg acgtatggcg ctgactgtgt gcatgcgcgc gaaccgcccg tcctaacggg
     2581 ccccgtttcc acctcaatcg atcgtcctca tgacaaccat caagaccgtc ggcgtgctcg
     2641 gcgccggact
//
LOCUS       DASUCJ010001209         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_669955, whole genome shotgun sequence.
ACCESSION   DASUCJ010001209 DASUCJ010000000
VERSION     DASUCJ010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669955"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFU90_14115"
     CDS             <1..400
                     /locus_tag="VFU90_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480976.1"
                     /translation="LRYRAFCMLFTGDAGAAAERRFLGEGADLRCQVLKVGHHGSAYS
                     STPAFIAAVHPRYAVVSVGRHNLFGHPAPATLETLRAAGATIYRTDEDGAVTITTDGT
                     DFAATPFLANRRAPAESAFGASPEPPGDGR"
     gene            390..1751
                     /locus_tag="VFU90_14120"
     CDS             390..1751
                     /locus_tag="VFU90_14120"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEU5480977.1"
                     /translation="MAGRPRSLQSGAFVALLTAALACLLAFVTLWRYRIFRTDVDLGI
                     FTQVIGSLGHGFSSTAEGGVNHLLVHWSPIVAFGWPFLRAFGPAGLQVFQALLVAATL
                     IPIWGLARARFGAAAAFGVVAVASLYPTLWGNGVGDFHEMAFVPLLSAALVYALDRRK
                     WTLGIACALLLACTKEDQFVTLAWIGAAIALTRRGDREQRRFGATVFFIALAGAFAYF
                     GVVRNLLQPHVPYLSFRFFDWSGEPSGPAALGAALLPRVRYVAIVLVPLAFLPCVSRY
                     GLFLVPGLVEILASHEPVTLAPGAHYSALLTGYALAAFVDGLSRIERRRPRALPAALA
                     VAAVASLAIEIFASPMEYWYYLYRVPNARDALLDARLRALPAGAGVGAEGEVFSHLGL
                     DPAASIGMNGQQWFVYDATHYSTRWHDVDQPAVRRLLAERAYTVVDDAGGVVVLRRAS
                     KRR"
     gene            1822..>2620
                     /locus_tag="VFU90_14125"
     CDS             1822..>2620
                     /locus_tag="VFU90_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480978.1"
                     /translation="MNLRIAVPFARPFACATLLTLLASGCSSGSAGLVPAFRAAPAAV
                     QPQAQSEHLYVADYAAVTVYAPKGGSPLRTIGNLEPSSIAFDAAGNLYVANVPVRGNG
                     NVTVFRAGKNSVLRKITQSLDDPRAMIFDASGNLYVANSYFRVAEFAPGQNSPMRYYK
                     VFYGSAMLFDAAGRFYAASDPSPYGGQGGSKILVYSAAGGHLYTITRGLNSPVSLALS
                     PSGNVFVANFAGNDVAIYPPGKTKLLRAISKGVKGPYALRFDNSGNLY"
BASE COUNT          363 a          916 c          835 g          506 t
ORIGIN      
        1 gctgcgctac cgagctttct gcatgctctt caccggcgat gcgggagccg ccgcggaacg
       61 ccgctttctc ggcgaaggcg ccgacttacg atgccaagtt ctaaaggtcg gccaccacgg
      121 ctccgcctat tcgtcgacgc cggccttcat cgccgcggtg catccccgct atgccgtcgt
      181 ctcggtcggc cgccacaatc tcttcggcca tccggcgccc gcgacgctgg aaacgctgcg
      241 cgccgccggc gccacgatct accgcaccga cgaagacggc gcggttacga ttacgaccga
      301 cggcacggat ttcgcggcga cgccgtttct cgcaaaccgc cgcgcgccgg ccgagagtgc
      361 attcggcgct tctcccgaac ctccgggaga tggcaggtag gcctcgttcg ctgcaatcgg
      421 gcgccttcgt ggcgctgctg acggctgctt tggcctgcct cctcgcgttc gtaacgcttt
      481 ggcgctatcg gatctttcgc accgacgtcg atctcggaat tttcacgcaa gtgatcggca
      541 gcctcgggca cggcttctcg agcactgccg aaggcggcgt caatcacctg ttggtgcatt
      601 ggtcgccgat cgtcgcattc ggctggccgt ttctgcgcgc gttcggaccg gcgggattgc
      661 aggtttttca agcgcttctc gtcgccgcca cgctgattcc gatttggggt ctggcgcgcg
      721 cgcgattcgg cgccgcagcc gcgttcggcg tcgtcgcggt ggcgtcgctc tacccgacgc
      781 tgtggggcaa cggcgtcggc gacttccacg agatggcctt cgtgccgctc ttgtcggctg
      841 cgctggtgta cgcactcgac cggcggaaat ggacgctggg gatcgcctgc gcgctgctgc
      901 tggcgtgcac caaagaagac cagttcgtaa ccttggcgtg gatcggggcc gcgatcgctt
      961 tgacgcgccg cggcgaccgc gagcagcgcc gcttcggcgc gaccgtgttc ttcatcgcgc
     1021 tcgccggcgc gttcgcgtac ttcggcgtgg ttcgcaatct gctgcagccg cacgtgccct
     1081 acttatcatt tcgcttcttc gattggagcg gcgagccaag cgggccggcc gcgctcggcg
     1141 cggcgctgct tcctcgcgtc cgctacgtcg cgatcgtgct cgtcccgctc gcgtttctgc
     1201 cgtgcgtctc gcgctacggc ctcttcctcg tgccgggtct ggtcgagatc ttggcttcgc
     1261 acgagccggt caccctcgcg cccggcgcgc actacagcgc gttgctcacc ggctacgcgc
     1321 tggcggcctt cgtcgacggc ctgtcgcgga tcgaacggcg gcggccgcgg gcgttgccgg
     1381 ccgcgctcgc cgtggccgcg gtcgcttcgc tggcgatcga gatctttgcc agcccaatgg
     1441 agtactggta ctacttgtat cgcgtgccga atgcccggga cgccctgctc gatgcaaggc
     1501 tgcgcgcgct cccggccggc gccggcgtcg gcgccgaagg cgaggtgttt tcgcacttgg
     1561 gtttggatcc cgccgcctcg atcgggatga acggtcagca gtggttcgtc tacgacgcga
     1621 cgcactattc gacgcgctgg cacgacgtcg accagccggc cgtgcggcgc ttgctcgcgg
     1681 aacgcgctta caccgtcgtc gatgacgcgg gcggcgtggt cgtacttcgg cgcgcgtcca
     1741 aacgccgcta gcgagcttct tgcgaacggc gctcgccggg agcgaggcct cgcgggccga
     1801 gggccgaact tctccggccc tatgaacctt cgaatcgccg tgccgttcgc acgcccgttc
     1861 gcctgcgcaa cgctgctaac gctcctggcg agcggatgct ccagcggatc ggccggtctc
     1921 gtaccggcgt ttcgagccgc gcccgccgcg gtccaaccgc aagcccagag cgaacacctt
     1981 tacgtcgccg actacgccgc ggtgacggtg tacgcaccca agggcggctc gccgctgcgc
     2041 acgatcggaa atctcgagcc ctcgtccatc gccttcgacg ccgccggaaa cttgtacgtt
     2101 gccaacgtgc ccgtgcgcgg gaacggaaac gttaccgttt tccgcgccgg gaagaactcg
     2161 gtcttgcgca agatcaccca gagcttggac gatccgcgcg cgatgatctt cgatgcatcc
     2221 ggcaatctgt acgtcgcgaa ttcgtacttc cgcgtcgccg aattcgcgcc gggacagaac
     2281 tcacccatgc gctactacaa ggttttctac ggttcggcca tgctgttcga cgccgccgga
     2341 cggttctacg cggcctccga tccgtcgcct tacggcggcc agggcggctc caaaatcctc
     2401 gtctacagcg ccgcgggagg ccacctctac acgattaccc gcggcctgaa cagcccggtg
     2461 tcgctggccc tcagtccgtc cgggaacgtg ttcgtcgcca actttgcggg gaacgacgta
     2521 gcgatttatc cgccggggaa aaccaaactg ctgcgcgcga tttcaaaggg cgtcaaaggg
     2581 ccgtacgctc tgcgcttcga caactccgga aatctctacg
//
LOCUS       DASUCJ010001210         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_287727, whole genome shotgun sequence.
ACCESSION   DASUCJ010001210 DASUCJ010000000
VERSION     DASUCJ010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287727"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFU90_14130"
     CDS             complement(<1..850)
                     /locus_tag="VFU90_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5480979.1"
                     /translation="MSTFLRATEIPTHGARTPPASWYTSPVTYALELDRIFHKRWLSA
                     CRASDVINAGDYVVRTIGTESVIITRDGEGAVRAFHNVCRHRGTRLCADAKGHFASGI
                     QCPYHAWTYALDGTLTGAPHMGGTEGFDRRDWPLHAVPIAEWEGWVFINLDRDPEPFA
                     SAFAPLISRFERFRLPTLEPARRIDYDVRANWKLIHQNYSECYHCSPVHPALVRLTPA
                     NSGENDLYDGPFLGGFMVIMQPGGSMSMSGRACGLPVGDLPSDDLQRVYYYSIFPNML
                     LSLHPDY"
     gene            988..>1521
                     /locus_tag="VFU90_14135"
     CDS             988..>1521
                     /locus_tag="VFU90_14135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5480980.1"
                     /translation="MPVTDITILLFLGALAAGLLGALTGLGGGAIIVPLLTILFHVDI
                     RYAVGTSLVSVIATSSGAAAAYVKEGYSNIRVGMLLEIATTIGAVTGAYIAALVSPTA
                     VAVIFGIVLLHTAWTSTRPRVEHIAGGPSDPIALRFRLDSTYPTANGPQHYQVQRVRL
                     GCGLMYIAGVLSGLLGIG"
BASE COUNT          246 a          511 c          489 g          275 t
ORIGIN      
        1 cgtagtcggg gtggaggctg agcagcatgt tcgggaaaat cgagtagtag tacacgcgct
       61 gcaggtcgtc gctcggcaga tcgcccaccg gcagaccgca cgcgcgtccg ctcatgctca
      121 tgctgccacc cggctgcatg atcaccatga agccgccgag gaacggcccg tcgtagagat
      181 cgttctcgcc gctgttcgcc ggcgtaaggc ggacgagcgc cggatgcacc ggcgaacagt
      241 gatagcactc ggaatagttc tgatggatga gcttccagtt cgcgcgcacg tcgtagtcga
      301 tgcgacgcgc cggctcgagc gtcgggagac gaaagcgctc gaaccgcgag ataagcggcg
      361 cgaacgcact ggcgaacggt tccgggtcgc ggtcgagatt gatgaacacc cagccttccc
      421 actccgctat gggaacggcg tgcaacggcc agtcgcgccg gtcgaacccc tcggtaccgc
      481 ccatgtgcgg cgcgccggtc aacgtgccgt ccagcgcgta cgtccatgcg tgatatggac
      541 actggatgcc ggacgcgaag tgccccttcg cgtccgcgca caggcgcgtt ccgcgatgcc
      601 ggcacacgtt gtggaacgcc cgcactgcgc cctcgccgtc gcgcgtgatg atcacgctct
      661 ccgtgccaat cgtgcgcacg acatagtcgc ccgcgttgat gacgtcggat gcccggcacg
      721 ccgagagcca tcgcttgtgg aaaatgcgat ccagctccag cgcgtaggtg acgggcgacg
      781 tataccacga cgcgggcggt gtccgtgcgc cgtgcgtcgg aatttcggtc gctcgcaaga
      841 acgtgctcac ctgcgtcctc cttcacggac gggtcacgct ccaatgtggc gcgtcgcccg
      901 tcgccgcggc aaccggtagt gcgcgacagt cgcacgcgct agactatggc ttgcctaccc
      961 tgcccttgtt ccgcgcatca cgcacacatg cccgtcacgg acatcacgat tctcctgttt
     1021 ctgggcgcgc tcgccgccgg attgttgggc gcgctcacag gacttggcgg cggcgcgatc
     1081 atcgttccgc tgctcacgat tctcttccat gtcgacattc gctacgcggt cggcacgtcg
     1141 ctcgtgtcgg tgattgccac gtcgtcgggc gcggcggcgg cgtacgtcaa ggaaggatac
     1201 agcaacattc gcgtcggcat gctgctcgag atcgcgacga ccatcggcgc cgtgaccgga
     1261 gcatacatcg ccgccctcgt gtcgccgact gccgtcgccg tgatcttcgg catcgtgctc
     1321 ctccacacgg cgtggacatc gacgcgcccg cgtgtcgagc acatcgccgg tggtccgtcg
     1381 gatccgatcg cgctgcgttt ccggctcgac tcgacgtatc cgacagcgaa cggcccgcag
     1441 cactatcaag tgcagcgcgt gcggctcggt tgcggactca tgtacatcgc cggcgtgctc
     1501 tcgggattgt tgggcatcgg c
//
LOCUS       DASUCJ010001211         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_349195, whole genome shotgun sequence.
ACCESSION   DASUCJ010001211 DASUCJ010000000
VERSION     DASUCJ010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349195"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFU90_14140"
     CDS             complement(<1..1162)
                     /locus_tag="VFU90_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEU5480981.1"
                     /translation="MQALELDDIQSGVLRPRPTPFAAVYVGLRIDDRKAGRALMQRLS
                     DVVSSAAHPESPAGDTWISAALTFAGLKSLGVPPSSLDTFSPQFQQGMAARARALGDV
                     GASDPDRWEKPLGSPDLHVVITGIAPASERLEAALERARDVLHSSPGIAAIWRQDCHV
                     MPGEREPFGFKDGISHPAVEGSGIPGTNPHESPLKAGEFVLGYSNEMSDSTEIPQPDV
                     LGRNGSYVAFRKLYQDVAAFRRYLKVNAASAGEEELLAAKMMGRWRSGAPLALSPNAD
                     DPALGEDGARNNVFLFRSDDAIGYRTPLGSHIRRANPRDSAIAGLARIHRMIRRGTAY
                     GPPLPDGVLDDDGADRGLMFAFVGANLGRQFEFVQTEWANDSAFFAGRSEKDP"
     gene            complement(1169..>2113)
                     /locus_tag="VFU90_14145"
     CDS             complement(1169..>2113)
                     /locus_tag="VFU90_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480982.1"
                     /translation="RRFSVYWSWSYPWEANRDVRELDNRFSTITEVRRVAWPAYETEP
                     YSLDNFLQGIDGTLELFHLSLVLFQAQVGEATGQPVAVYQRVDQAGQMLPIDERILAD
                     TDTLMIFGLDHLPANLEAAPEEIEAIETFLRREGTCLILGPHHDVGAGPDLKVRDIEY
                     RHHGDPLVPRQQRFTTYTRSLMKGLGVPVENRWGLRPATLPGTHKPVPLAIAGDLDER
                     GWLEGVETFVFHMHLPHYAVTAGDPKTIRVLARQPIDVTRPHPFVDEGNRELNMFLWM
                     PPSGRRAGDILFADSTIFTTLFGGDESLSRFWKNLATK"
BASE COUNT          384 a          679 c          673 g          377 t
ORIGIN      
        1 ccggatcctt ttccgagcga cccgcaaaaa atgcgctgtc gttggcccat tcggtctgta
       61 cgaactcgaa ttgccgcccg agattcgcgc cgacgaacgc aaacatcaag ccccgatcgg
      121 ccccgtcgtc gtcgagcacg ccgtcgggca gcggcggccc gtacgccgtg ccgcggcgaa
      181 tcatgcgatg aatgcgcgcc aaccccgcaa tcgccgaatc gcgcggattg gcgcggcgga
      241 tgtgcgaccc gagcggcgtc cgatatccga tcgcgtcgtc cgaacggaac aaaaaaacat
      301 tgttgcgggc gccgtcttcg cccaaggcag ggtcgtccgc attcgggctc aacgccagcg
      361 gggcgccgct gcgccagcgc cccatcattt tcgcggccaa cagctcttcc tcgccggcgg
      421 atgcggcgtt gaccttgaga tatcgtcgaa aggcagcgac gtcctgatag agcttgcgaa
      481 acgcgacgta gcttccgttg cgcccgagca cgtcaggttg cggaatctcg gtggagtcgc
      541 tcatttcgtt ggaatatccg aggacgaact cacccgcctt gagcgggctt tcgtgcggat
      601 tggttccggg aattccgctg ccctcaacgg cggggtggct gatgccgtcc ttgaatccaa
      661 agggttcgcg ctcgcccggc attacgtggc aatcttgccg ccagatcgct gcgattccgg
      721 gcgaggagtg cagaacgtcg cgcgctcgtt ccagggccgc ttcgagccgt tcgctagccg
      781 gcgcaatccc ggtgatgacg acgtgaagat cgggcgaacc cagcggcttc tcccagcgat
      841 cggggtcgct cgctccgacg tccccgagcg ctcgcgcgcg tgccgccatt ccctgttgaa
      901 actggggcga gaacgtatcg agggacgatg gcggtacgcc cagcgatttc agccccgcga
      961 aggtcagcgc cgcactgatc cacgtatccc cggcggggct ttccgggtgg gcggccgacg
     1021 atacgacgtc gctcagccgc tgcatgagcg cgcgcccggc ttttcgatcg tcgatccgca
     1081 acccgacgta taccgcggca aacggcgtgg gacgcggccg cagcacgccg ctctgaatgt
     1141 cgtccagctc gagagcctgc atgtcgtcct acttcgtagc gagattcttc caaaaacgcg
     1201 acaggctctc gtctccgccg aacagagtcg taaagatcgt cgaatcggcg aagaggatat
     1261 cgccggcgcg ccgtccggaa ggcggcatcc acaaaaacat attgagctcg cgatttcctt
     1321 cgtcgacgaa cgggtgcggc cgcgttacgt cgatcggttg acgggccaac acccgaatcg
     1381 ttttgggatc gcccgccgtc accgcgtagt gcggcaagtg catgtggaag acgaacgttt
     1441 cgacgccttc gagccagccg cgttcgtcca agtcgccggc aatcgcgagc ggcaccggct
     1501 tgtgcgtccc cggcagggta gcggggcgca agccccagcg attttcgacc gggacgccga
     1561 gccctttcat caacgatcgc gtgtacgtcg taaaccgttg ctgccgaggg acgagcggat
     1621 cgccgtgatg ccggtactcg atatcgcgca ccttcaagtc cgggccggcg ccgacatcgt
     1681 ggtgggggcc gagaatcaga cacgtacctt cgcggcgcag aaaagtctcg atcgcctcga
     1741 tttcctcggg agcggcctcg agatttgccg gcaaatgatc gaggccgaag atcatcagcg
     1801 tgtcggtgtc ggccagaatc cgctcgtcga tggggagcat ttggccggct tgatcgacgc
     1861 gctggtagac ggcgaccggc tgtccggtcg cctcgccgac ctgcgcctgg aagaggacca
     1921 gcgacaaatg gaacagctcg agcgttccat cgatgccttg cagaaagttg tccaacgagt
     1981 acggttcggt ctcgtacgcc ggccaggcga cgcgccggac ttcggtgatc gtcgaaaagc
     2041 gattgtcgag ctcccggacg tcgcgattag cctcccaagg atagctccag ctccagtaga
     2101 cgctgaagcg gcg
//
LOCUS       DASUCJ010001212         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_563079, whole genome shotgun sequence.
ACCESSION   DASUCJ010001212 DASUCJ010000000
VERSION     DASUCJ010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563079"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1449
                     /locus_tag="VFU90_14150"
     CDS             <1..>1449
                     /locus_tag="VFU90_14150"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5480983.1"
                     /translation="FVGSLFSLQPGNTYGVRVTLSDPDGVTGAAAVTENLTTRAENAP
                     FTPSRTLYVAPNGSDGNSGTNPSQPLKTIQHAADISEAGDLVSIAAGIYYEDVHVSTS
                     GADSAPIVFRGAQGAIVDGADPAIVAGVTWTNRGGGVWSYATGYSTDLVVTDQGRLFD
                     YGSLADLQTLEAGAPGGSFADGTNVYVKFSDGSSPAQRHMNVSRFDAGFYVDGAGNIR
                     IENLEIRYYGSDAFGRAVYLRFANDCEISGSNMHENGATAVWIKGGARNVVEDNDIGD
                     SSIIGWPWDLTHASTAQNTGVYFTDNNGQGNVIRRNRLHGTYDGMHPCGDSAPDNAFT
                     SETDVYDNVIWSHSDDAIESEGYCSNVRIWNNTISDSLMAVAVAPAAPGPTWIVRNTG
                     YNIGNVPSVSQFEQTPSSIKINSDYPQVVGPLLVYHNTFLTTVAGADSLVLFNPGYNT
                     YIYSRNNVYVAPHNSLRKINTILLDFDFDDMYS"
BASE COUNT          276 a          521 c          434 g          218 t
ORIGIN      
        1 cttcgtcggg agcctcttct cgctgcagcc gggcaacacc tacggcgtgc gcgtcacgct
       61 gtccgatccc gacggcgtca cgggcgccgc agcggtcacc gaaaacctga cgacgcgcgc
      121 cgagaatgca ccgttcacgc cgtcgcgcac gctctacgtc gcgccgaacg gatcggacgg
      181 caactcgggc acgaatccgt cgcagccgct gaagacgatc cagcacgcgg ccgacatctc
      241 tgaggccggc gatctcgtct cgatcgcggc cggcatctac tacgaggacg tgcacgtctc
      301 gacctccggc gccgactcgg cgccgatcgt gttccgcggc gcgcaaggcg cgatcgtcga
      361 cggtgccgat ccggcgatcg tcgccggcgt cacctggacg aaccgcggcg gcggcgtctg
      421 gtcgtacgcg acgggctact cgaccgatct cgtcgtcacc gatcagggac gcctcttcga
      481 ctacgggagc ctcgccgatc tgcagacact cgaagccggc gcgcccggcg gctcgtttgc
      541 cgacggcacg aatgtctacg tcaagttcag cgacggctcg tcgccggcgc agcggcacat
      601 gaacgtgtcg cgcttcgacg ccggcttcta cgtcgacggc gccggcaaca tccgcatcga
      661 gaatctcgag atccgctact acggcagcga cgcgttcggc cgcgcggtgt acctgcgttt
      721 cgcgaacgac tgcgagatca gcggcagcaa catgcacgag aacggcgcga ccgcggtctg
      781 gatcaagggc ggcgcgcgca acgtcgtcga ggacaacgac atcggcgatt cctcgatcat
      841 cggctggccg tgggacctca cccacgcgag caccgcgcag aacaccggcg tctacttcac
      901 cgacaacaac ggccagggca acgtcatccg tcgcaaccgc ctgcacggca cctacgacgg
      961 catgcatccc tgcggcgact cggcgcccga caacgcgttc acgagcgaga ccgacgtcta
     1021 cgacaacgtg atctggagcc acagcgacga tgcgatcgag tccgaaggct actgctcgaa
     1081 cgtgcgcatc tggaacaaca cgatcagcga cagcctgatg gccgtcgcgg tcgcgccggc
     1141 ggcgccaggg ccgacctgga tcgtgcgcaa caccggctac aacatcggca acgtcccgtc
     1201 cgtttcgcag ttcgagcaga cgccgagcag catcaagatc aacagcgact acccgcaagt
     1261 cgtcggcccg ctgctggtct accacaacac gttcctgacg accgtcgccg gcgccgattc
     1321 gctggtgctg ttcaatcccg gctacaacac gtacatctac agccgcaaca acgtctacgt
     1381 cgcgccgcac aattcgctgc gcaagatcaa cacgatcctg ctcgacttcg acttcgacga
     1441 catgtactc
//
LOCUS       DASUCJ010001213         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_792179, whole genome shotgun sequence.
ACCESSION   DASUCJ010001213 DASUCJ010000000
VERSION     DASUCJ010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792179"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1092)
                     /locus_tag="VFU90_14155"
     CDS             complement(<1..>1092)
                     /locus_tag="VFU90_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480984.1"
                     /translation="AGLFQCNQNNFCYGSSGTLGQFDPSTHAFTEIDLPTYEGLTAYQ
                     TADGAVWVSLSHTDGTTQKTDGSVGRISGAFTSANLNVIALPTASPDPGNSRPRSFAL
                     GADGNLYLTGNSDHKIYKIPTTNTSTANITAVPVPNYTPGPGAALPWTSAAYPEGIAA
                     GTDGKLYVANNSAIAPNVLQYDTAGATFTPFALPGVVQSQAPRYIIAGSDGNLYQSSS
                     GTCLTMPCGGALNALTTAGVFTPIGLPDGYSFPDDLARGTGFIAFADLGEAALGTYDF
                     AAKEVRDYPIEAYTSPCCSVWKAPQGVTVASDGSIWFVTYGRVSTNGPVALGHVIMTQ
                     NWSVWPSQSITLTGAGSQAAQLVAVMENG"
BASE COUNT          179 a          346 c          358 g          209 t
ORIGIN      
        1 cgccgttttc catgacggcg acgagttgcg cggcttgcga tccggcgccg gtgagcgtga
       61 tgctctggga cggccagacg ctccagtttt gcgtcatgat cacgtgtcca agcgcaaccg
      121 gcccattggt gctcacgcgt ccatacgtga cgaaccagat ggatccgtcg cttgcgaccg
      181 tgacgccctg cggcgctttc caaacgctgc agcagggcga ggtatacgcc tcgattggat
      241 aatcgcgcac ctcttttgct gcgaagtcgt acgtgcccag cgcggcttcg ccgagatcgg
      301 cgaacgcgat gaacccggta ccccgtgcga gatcgtcggg gaacgaatat ccgtccggca
      361 aaccaatcgg cgtaaagacg cccgccgtcg tcaacgcgtt gagcgcgccg ccgcacggca
      421 tcgtcaggca cgttcccgac gaggactgat acaggttgcc gtccgagccc gcgatgatgt
      481 accgcggggc ctgagactgc accacgcccg gcagcgcaaa cggcgtgaac gtagcacccg
      541 ccgtatcgta ctgcagcacg ttcggcgcga tcgccgaatt gtttgcgacg tacagcttgc
      601 cgtccgtgcc ggcggcgatg ccttccggat atgccgctga cgtccacggg agcgccgctc
      661 ccggccccgg cgtgtagttc ggcaccggta ccgcggtgat gttcgcggtc gacgtgttag
      721 tcgttgggat tttgtagatc ttgtgatccg agttgccggt caggtagaga ttcccgtccg
      781 cgcccagcgc gaacgagcgc ggccgcgagt tacccgggtc gggcgaagcc gtgggaagcg
      841 cgatcacgtt cagattcgcg ctggtgaacg cgccgctgat gcggccgacg gaaccgtccg
      901 ttttctgcgt ggtgccgtcc gtgtgcgaga gcgaaaccca cactgcgccg tcggcggtct
      961 ggtacgccgt cagtccttcg tacgtcggca gatcgatctc ggtgaacgcg tgcgtgctcg
     1021 gatcaaattg ccccagcgtt ccgctgctgc cgtagcagaa gttgttctgg ttgcactgga
     1081 acaggccggc tg
//
LOCUS       DASUCJ010001214         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1174467, whole genome shotgun sequence.
ACCESSION   DASUCJ010001214 DASUCJ010000000
VERSION     DASUCJ010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174467"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1364
                     /locus_tag="VFU90_14160"
     CDS             60..1364
                     /locus_tag="VFU90_14160"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU5480985.1"
                     /translation="MRRKFAALLALLFAAAPLRAPALELHSIEQAAADYRSPNAQLQE
                     MFRAALLDTSKLAQYAPDGTAYVATGDIPAEWLRDASAQSRPYLFFTKDDPDVRRLLA
                     AIIARMVKYVQIDPYANAYTLDYRVWEQKFELDSLAYPTALAWSYWKTTHDTSIFTPD
                     FAKMLDAVLATMQREQDHPRNSRYTHKELPNNGAGRPVGYTGMIWTGFRPSDDACYYN
                     FLIPSEMFAVVALGDMAEIERDVYHNVVKAHEAKALRDEVQRGIQTYGLVLVPKYGYI
                     YAYEVDGLGHAILTDDANIPSLLSAPYIGYTTDNDRYYDNTRKFLLSQDNPSFYQGRD
                     ARGIGSYHTPDHWVWPLALIVQGMTATSASEKQDVLAQLLASDPGDHLLHESFNPDDP
                     KQFTRQDFGWPNALFSEFVMTQFQGVAPIPMGDTSDLEFRSE"
BASE COUNT          278 a          484 c          485 g          253 t
ORIGIN      
        1 tacttcggcc gctaccgcga gctgccggcg ctctacgcgc acggcacgtg gccgggctga
       61 tgcgtagaaa atttgccgcg ctgctggcgc tgctgttcgc ggccgcgccg ctgcgcgcgc
      121 cggcgctcga gctgcactcc atcgaacagg cggcggccga ctaccgcagc cccaacgccc
      181 agttgcagga gatgttccgg gcggcgctgc tggacacgag caagctcgcg cagtacgccc
      241 ccgacggcac ggcctacgtc gcgaccggcg acattccggc cgagtggctg cgcgacgcga
      301 gcgcgcagtc gcgtccctac ctattcttca cgaaggacga tcccgacgtg cgccggctgc
      361 tggccgcgat catcgcgcgg atggtgaagt acgtccagat cgacccgtac gccaacgcct
      421 acacgctcga ctaccgcgtc tgggagcaga agttcgagct ggattcgctc gcctatccga
      481 cggcgctggc gtggagctat tggaagacca cgcacgacac gtcgatcttc acgcccgatt
      541 tcgcgaagat gctcgatgcg gtgctggcga cgatgcagcg cgagcaagac catccacgca
      601 actcgcgcta cacgcacaag gagctgccga acaacggtgc gggccggccg gtcggctaca
      661 ccgggatgat ttggacggga ttccggccgt ccgacgatgc ctgctactac aacttcctga
      721 ttccgtcgga gatgttcgcg gtggtggcgc tgggcgacat ggccgagatc gagcgcgacg
      781 tctaccataa cgtggtgaag gcgcacgaag ccaaggcgct gcgcgacgaa gtgcagcgcg
      841 gcatccagac ctacggtctg gtgctggtgc cgaagtacgg ctacatctac gcctacgaag
      901 tggacgggct cgggcacgcg attttgaccg acgacgccaa cattccgagc ttgctgtcgg
      961 cgccgtacat cggctacacc accgacaacg accgttacta cgacaacacc cgcaagttct
     1021 tactgtcgca agacaatccg tcgttctacc aaggccgcga cgcgcgcgga atcggcagct
     1081 atcatacgcc ggaccactgg gtctggccgc tggctttgat cgtgcagggg atgaccgcga
     1141 cctcggcgtc ggagaagcag gacgtgctcg cgcagctgct ggcgagcgat ccgggggatc
     1201 atctgctgca cgaatcgttc aatccggacg atcccaaaca gtttacccgc caggatttcg
     1261 gctggccgaa cgcgctgttc tcggagttcg tgatgacgca gttccagggc gtcgcgccga
     1321 tcccgatggg cgacaccagc gacttggagt ttcgatctga gtaattccgg ccgcaaggcc
     1381 gacgtcatcg tcggaattca gtggggcgat gaggggaaag gtcggatcgt cgatctgtac
     1441 gcgcgcgatt acgacgtgat cgcgcgcttc ggcggcggcg acaacgccgg gcactcgatc
//
LOCUS       DASUCJ010001215         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_654896, whole genome shotgun sequence.
ACCESSION   DASUCJ010001215 DASUCJ010000000
VERSION     DASUCJ010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654896"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFU90_14165"
     CDS             <1..294
                     /locus_tag="VFU90_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5480986.1"
                     /translation="IEVELAKLMLQKAAALYDGGDDPGAAEAANIAKYAAGEATTRAV
                     DQAVQCLGGNGLTKEYGIAAAVTASRLARIAPVSREMVLNFVAQTALGLPLSY"
     gene            334..1119
                     /locus_tag="VFU90_14170"
     CDS             334..1119
                     /locus_tag="VFU90_14170"
                     /inference="COORDINATES: protein motif:HMM:NF005879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEU5480987.1"
                     /translation="MSASAKSRMYVDYEVRASTARRTLNSPHNRNALSSALVEELHQA
                     LRDAAADPKARVVVLGHTGGTFCAGADLSEASEGGPDVRTRQLTELLRGILELPLPVV
                     GAIDGHVRAGGFGLVGACDIIVAGRNSTFALTEARIGVAPAIISLTLLPKMTPRSVGR
                     YFLTGEKFGADEAERIGLVTIAADDVEATVGRITAELGRGSPQGLRESKALTTAEMLD
                     GFDRRAEELSAQSARLFASDEAREGMLAFLQKRPPAWITETAK"
     gene            complement(1180..>1913)
                     /locus_tag="VFU90_14175"
     CDS             complement(1180..>1913)
                     /locus_tag="VFU90_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5480988.1"
                     /translation="RAVVEWISEVRPDLVALQEVVRTSQLCQASWIAEKTGMSPVFGA
                     AGSYSGAEFGNAVLSRFPVLGSRCRRLHEGSDGDAPRAIVTADVEADGQRVSFSSTHL
                     SYRFDDGWARQEQVREIADFVSADSADYPLIVCGDFNAEPAATEVRFIKGLHALDGQS
                     FHLFDAFEVAHPGEFGYTWSNANPFAAEEREPDRRIDYIFVGVRGDDGGGQILDAKVV
                     CDQPRAGVWPSDHFGVAAVLSCPSC"
BASE COUNT          357 a          633 c          621 g          302 t
ORIGIN      
        1 atcgaagtcg agctggccaa actgatgctg cagaaagctg ccgcacttta cgacggcggg
       61 gacgacccag gcgcggcgga ggcggcgaac atcgccaagt acgctgccgg cgaagcgacg
      121 acccgcgcgg tcgaccaggc cgttcaatgc ctgggcggca acggattgac gaaggagtac
      181 ggcatcgcgg ccgcggtcac cgcgtcgcgg ctggcccgca tcgcgccggt gagccgcgag
      241 atggtgctca acttcgtcgc gcagaccgcg ctgggcctgc cgctctcgta ctgatggcca
      301 ccgatgagcg cttgcgcgaa gagaagatga ccgatgagcg ctagcgcgaa gagcagaatg
      361 tacgtcgact acgaggtccg agcgtcaaca gcacggcgca cgctcaactc gccgcacaac
      421 cgcaacgcgc tgtcgtcggc actcgtcgag gagttgcatc aggcgctgcg tgatgcggcc
      481 gccgacccga aagcgcgtgt cgtggtgctc ggtcacaccg gcggcacatt ctgcgcgggc
      541 gcggatttgt ccgaggcatc cgaaggcgga cccgacgtcc ggacccgcca attgaccgaa
      601 ttactaaggg ggatcctcga actgccgctg ccggtcgtcg gcgcgatcga cgggcacgtg
      661 cgcgccggcg ggttcgggct cgtcggcgcc tgcgacatca tcgtcgccgg ccgcaacagc
      721 acgttcgcgc tcaccgaggc gcgcatcggg gttgcgccgg cgatcatctc gctcacgttg
      781 ttgccgaaga tgaccccgcg gtccgtcggc cggtacttcc tgacgggcga gaagttcggc
      841 gccgacgaag ccgaacggat cgggctggtg acgatcgcgg ccgacgatgt ggaagccacg
      901 gtcggcagga tcaccgccga gctcggccgc ggctcgccgc aggggctgcg tgagtcgaag
      961 gctctgacga cggccgagat gctcgacgga ttcgaccgac gcgccgagga gctcagcgcg
     1021 cagtcggcgc ggctgttcgc ctccgacgag gcacgtgagg gcatgctcgc gttcctgcag
     1081 aagcggccac cggcctggat caccgagact gcaaaatagt cgcgaaaatg cgagtgcggc
     1141 tctgccgaaa tacagttcga ccccggcacg ccacgtcggt tagcacgacg ggcacgaaag
     1201 caccgcggcc acaccgaagt ggtcgctcgg ccataccccg gcgcgtggct ggtcgcacac
     1261 caccttggcg tccagaattt gtcccccacc gtcgtccccg cggactccga cgaagatgta
     1321 gtcaattcgc cgatccggct ctcgttcctc ggctgcaaac gggttggcgt tgctccacgt
     1381 ataaccaaac tcgccggggt gggcaacttc gaacgcatca aaaaggtgaa agctttggcc
     1441 atcaagggcg tgcaggccct tgatgaaccg cacttctgtc gctgcgggtt cggcgttgaa
     1501 gtcgccgcac acaatcaatg gatagtccgc cgaatccgcg ctcacgaagt cggcgatctc
     1561 ccggacctgt tcttggcgtg cccagccgtc gtcgaatcga tacgacaggt gcgtcgagga
     1621 gaacgaaacc cgttgtccgt cggcttcgac gtctgccgtc acgatcgcgc gcggcgcatc
     1681 gccgtccgat ccttcatgaa gacgtcggca ccgcgatccc aggacgggga accggctgag
     1741 caccgcgttg ccgaactcgg cacccgaata tgaacctgca gcaccgaaaa ctggcgacat
     1801 accggttttc tcggcgatcc acgatgcctg acacagctgg ctcgtccgga ccacctcttg
     1861 cagcgcaaca agatctggac gtacttcgct gatccactcg acgacggccc ttc
//
LOCUS       DASUCJ010001216         1361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_165714, whole genome shotgun sequence.
ACCESSION   DASUCJ010001216 DASUCJ010000000
VERSION     DASUCJ010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165714"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFU90_14180"
     CDS             <1..464
                     /locus_tag="VFU90_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5480989.1"
                     /translation="ADAPLHVHDGWPPSDALPVDVFAGPAVVCSLDRLDALSDTRVER
                     LLLRTGASTAGGAFPADWPALTVRQIEALLARGLRLLGVDAPSVDARHSTALEVHHAL
                     FAGGGYNLENLDLRDVRDGAYDLVALPLKVIGLDAAPVRAALRPDASTPLR"
     gene            complement(482..874)
                     /locus_tag="VFU90_14185"
     CDS             complement(482..874)
                     /locus_tag="VFU90_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480990.1"
                     /translation="MPNVTKKLTPVLMVDAIEPCLPLWTQRLGWTKTVEVPEGDRLGF
                     VILEKDGVEVMYQTWASIENDLGTRPVRPVGTSVGLFIEVSDLDEIERQIAGEPLVLP
                     RRRTFYGMDEVGIREAGGHTMVFAQRAG"
     gene            955..>1361
                     /locus_tag="VFU90_14190"
     CDS             955..>1361
                     /locus_tag="VFU90_14190"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HEU5480991.1"
                     /translation="MNESGVPEGYVEHAPPPDLAAAVVCAWTRRVSGIVDVAVHRVLP
                     DGCCDILFVFGDGDVASATIVGAMTRPILVHGPHPRLYVGVRLSPGYAQSAFRLPASD
                     LTDQNVEYELLVPEAAEHVDALAAASDAQCVAIA"
BASE COUNT          199 a          504 c          419 g          239 t
ORIGIN      
        1 acgccgatgc cccgctccac gtgcacgacg gctggccgcc gtccgatgca ctgccggtcg
       61 acgtctttgc cggtcccgcc gtcgtctgct cgctcgaccg gctcgatgcg ctttcggaca
      121 cgcgcgtcga gcgcctgctg ctccgcaccg gcgcgtcgac cgccggcggt gcatttcccg
      181 ccgactggcc ggcgctcacc gttcggcaga tcgaggcgct actggcgcgg ggactccgcc
      241 tcctgggagt ggacgcaccg tcggtggatg cgcggcacag cacggcgctc gaggtgcacc
      301 acgcgctctt cgccggcggc gggtacaacc tcgagaacct cgatctccgc gacgtgcgcg
      361 acggcgccta cgatctcgtg gcgctgccgc tcaaggtgat cggcttggac gccgcgccgg
      421 tacgtgccgc gcttcggcct gacgcaagca cgccgctgcg ttaggcccgg cgcgcgccgc
      481 ctcagcccgc ccgttgggcg aataccatgg tgtggccgcc ggcctccctg atccctactt
      541 catccatccc gtagaacgtg cggcggcgcg gcagcaccag cggctccccg gcaatctggc
      601 gctcgatctc atccaggtcc gacacctcga tgaacaaccc caccgacgtc cccaccggcc
      661 gcaccggcct cgtgcccaaa tcgttttcga tgctcgccca cgtctggtac atcacctcga
      721 caccatcttt ctcaagaatg acgaacccca gacggtcgcc ttccggcacc tccaccgtct
      781 tggtccaacc gaggcgctgt gtccacagcg gcaagcacgg ttcgatcgca tccaccatga
      841 gcacgggcgt caacttcttc gtaacgtttg gcatggtcat ctctctctcg gtttgcgtga
      901 cgcgaccaat ctggatgcga tccgcctgag ggtcttggaa aaatggaacc acacgtgaac
      961 gagagcggcg tgccggaagg atacgtcgag catgcgccgc ctccggacct cgccgccgcc
     1021 gtcgtctgcg cgtggacgcg tcgcgtcagc ggcatcgtcg atgtcgccgt gcatcgcgtg
     1081 ctgcccgacg ggtgctgcga tatcctgttc gtgttcggcg acggcgatgt cgcgagcgcg
     1141 acaatcgtgg gcgccatgac gcggccgatt ctcgtgcacg gtccgcaccc tcgattgtac
     1201 gtgggcgtcc gtctctcgcc aggctacgcc cagtcggcgt ttcgccttcc cgcatccgac
     1261 ctcaccgacc agaacgtgga atacgagctg ctcgtgccgg aggcggccga gcacgtcgat
     1321 gcactcgctg cagcgagcga cgcccagtgc gtcgccatcg c
//
LOCUS       DASUCJ010001217         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1021586, whole genome shotgun sequence.
ACCESSION   DASUCJ010001217 DASUCJ010000000
VERSION     DASUCJ010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021586"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFU90_14195"
     CDS             <1..1114
                     /locus_tag="VFU90_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480992.1"
                     /translation="DTSYNYDLSWEQRLKDTDWSFKLTPFYRSTRDQLQNFFIDPQGG
                     LESGLNVGHQVSYGLEFALQKGDFARDGWSGQLSYTFTHSRIQYQNFSGLNVNVIDQL
                     NYYVRQYNAFTHAGGGSPCYTYAGAPSSCGAKGVVTNPYYASAPEPLFDRNGWYTTYD
                     VIPGPAAGAFGYETPDVASLIVNYRHAKFAITPSLTYSSGSFYGSPTQWPGYNPAQCL
                     KPGNAWLSAHGNAADPAMCGGTGSFNLPLFIPDKYTNQFDNLGSFAEPWRMSLNLAME
                     YDVSPKATARVTLTNIVDHCGQRGYPWDNPYVCVYGNLPSGILYPAGNYYPNHNSPSP
                     PPQLQYPYSYVLNNNNTGFVGAVLPMEVNFALQLKL"
     gene            complement(1137..1481)
                     /locus_tag="VFU90_14200"
     CDS             complement(1137..1481)
                     /locus_tag="VFU90_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5480993.1"
                     /translation="MKRRLIALTLAAAAAAAPALAASPQMDGTLPMYPNARQDARESV
                     PASAIAKGVPLVLLTGDSVSTVDAWYKANAPKGCARRAAAQGVQYKCPGGSIQIYDHG
                     GTQIALVPAMGF"
     gene            complement(1526..>1899)
                     /locus_tag="VFU90_14205"
     CDS             complement(1526..>1899)
                     /locus_tag="VFU90_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEU5480994.1"
                     /translation="RAGLVAWALQHLHDAARYGSLATVAAACGGAAALSNLLNNLPVA
                     IVASYVAAHDPSGRLAYATIVGVDVGPNLTTAGSLATLLWLTVLDRRGVRVDPLEFTR
                     LGALVVPPVIAVTVAWLWLVR"
BASE COUNT          354 a          640 c          598 g          307 t
ORIGIN      
        1 cgacacgtcg tacaactacg acctgtcgtg ggagcagcgc ctcaaagaca ccgactggag
       61 ctttaagctc acaccgttct atcgcagcac ccgcgatcaa ctgcagaact ttttcatcga
      121 tccgcagggc ggactcgaat cggggctcaa cgtcgggcat caagtctcgt acgggctcga
      181 gttcgcgctg cagaaggggg acttcgcgcg ggacggctgg tcgggtcagc tgtcgtatac
      241 gtttacccac agccggatcc aatatcagaa cttctccggg ctgaacgtga acgtcatcga
      301 ccagctcaac tactacgtcc gccagtacaa cgcgtttacg cacgcgggcg gcggctcgcc
      361 gtgctacacc tacgccggcg cgccgtcgag ctgcggcgcc aaaggcgtcg tcaccaaccc
      421 ttactacgca tcggccccgg agccgctgtt cgaccgcaac ggctggtaca cgacctacga
      481 cgtcatcccc ggtcccgccg ccggcgcgtt cggctacgag acgcccgacg tggcgtcgct
      541 gatcgtcaac taccggcatg ccaagttcgc aatcacgccg tcgctgacgt acagttcggg
      601 aagtttctac ggttcgccga cgcagtggcc cggctacaac ccggcgcagt gccttaagcc
      661 gggcaacgcg tggctatcgg cccacggaaa cgccgccgat ccggccatgt gcggaggcac
      721 gggttcgttc aacctgccgc tgttcatccc ggacaagtac acgaatcagt tcgacaactt
      781 gggcagtttt gccgaaccgt ggcggatgtc gctgaacttg gcgatggagt acgacgtcag
      841 cccgaaggcg acggcccggg tgacgctcac gaacatcgtc gatcactgcg ggcagcgcgg
      901 gtatccctgg gacaacccgt acgtctgcgt gtacggcaac ctgccgtcgg gcatcctgta
      961 ccccgccggg aactattatc cgaatcacaa ttcgcccagc ccgcctcctc agctgcagta
     1021 tccgtactcg tacgtgctca acaacaacaa caccggtttt gtgggtgccg tgctgccgat
     1081 ggaagtgaac ttcgcactgc agctgaagct gtaagcggac gtgtagaaga agcggcttag
     1141 aaccccatcg ccggcaccaa ggcgatctgc gtaccgccgt ggtcgtagat ctgaatcgag
     1201 ccgccgggac acttgtactg cacgccttgg gccgccgcgc ggcgcgcgca gcctttgggc
     1261 gcgttcgctt tgtaccaggc atcgacggtc gaaaccgaat cgccggtgag cagcaccagc
     1321 ggtacgccct tggcgatcgc cgaagcggga acggattcgc gcgcgtcttg ccgtgcgtta
     1381 gggtacatcg gcaacgtgcc gtccatttgc ggcgacgccg ccaaagcggg tgcggccgcg
     1441 gcggccgctg ccagggtgag tgcgatcagt cgtcgtttca tgcgtccagc gtacgggcca
     1501 gccgtaccgg cggcgtgcgc ggccgctacc gcacgagcca gagccaagcg acggtaacgg
     1561 caattacggg cggcaccacg agcgcaccca aacgcgtgaa ctcgagcgga tcgacgcgca
     1621 cgccgcggcg gtcgagcacc gtcagccaga gcagcgtcgc cagcgatccg gcggtcgtga
     1681 ggttggggcc gacgtcgacg cccacgatcg tcgcgtaggc cagacggccg ctcggatcgt
     1741 gcgcggcgac gtacgacgcg acgatcgcga ccggcaggtt gttcagcaaa ttcgagagcg
     1801 ccgccgcgcc gccgcaggcg gcggcgacgg tcgccaaact cccgtaccgc gccgcgtcgt
     1861 gcaggtgctg caacgcccac gcaaccagac ccgcgcgcg
//
LOCUS       DASUCJ010001218         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1037006, whole genome shotgun sequence.
ACCESSION   DASUCJ010001218 DASUCJ010000000
VERSION     DASUCJ010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037006"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFU90_14210"
     CDS             <1..598
                     /locus_tag="VFU90_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5480995.1"
                     /translation="ALQVFGGMGYIEETGIAQQYRDVRIVTIYEGTTGIQALDLIGRK
                     LIRDMGATATAVGAKIAAVAKECAGSENADVKAIGAALAESLKALNETSQWIGMNAMA
                     DLNKAFACGVPYLRLWGVVAGGWQMARAAAIAARKIAAGDPEADFYQAKLATAKFYAT
                     HVLSQGAWFARQITEGSADVMGLSEEQLDLDRKMTANV"
     gene            600..2738
                     /locus_tag="VFU90_14215"
     CDS             600..2738
                     /locus_tag="VFU90_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5480996.1"
                     /translation="MEVRVRRQAGAGGKNVAVLTLDNPPVNALSFAFSERLLEAIAEA
                     EKDPGVSAVVFAGAGGIFSGGADVNDFNAEPSPDAKTVRDAIAAVERGTKTYVAAIDG
                     NALGGGFELALACDYRVATKAAKVGLPEIKLGLIPGAGGTQRLPRAIGAQAALEFMLK
                     GRSHTAAEAAALGLLDEVVDGDAVEAAIVLAQREAPQRRRLSQRRAVIGAGISPQAAP
                     FVVSQAHKMVPPEDRGGFAAHKLIDAVEAAVEMPFEFGIAREARLFEELARSAPSHAL
                     RHLFFAERELSKIPGLPPAEPLEIGNAGVVGAGTMGSGIAIAFAQVGIPVTVIDSNDE
                     AVDKARQTVMGMFMYQVQKGRLTQEEAWKRGQSITFTDDWSELAGADVVVEAVFEDLA
                     VKKDVFAKLDGIVKPEAILATNTSTLDVDAMAAATGRPEKVLGLHFFVPANIMPLLEI
                     VRGAATSPQTIATAFRLAKRLRKTAVLSGNAFGFIGNRMVFDYAREALALAEEGTPPA
                     RIDGVMKDFGFPMGPFAMSDLSGIDVAWHIAQARGGEGLGRTQVVQRLVESKRLGQKT
                     MAGYFKYDKSVGKGREPIPDAEVEALFAEEAKRAGIAPRAAGDEEIRERLLYALINRG
                     AYLLEEGVALRPGDIDVVYVYGYGFPPHRGGPMWYADETGVERVYATVERFARDFGPQ
                     WEPAPLLAAIAARGGTFAAHAAAEKEPAGV"
     gene            2731..3918
                     /locus_tag="VFU90_14220"
     CDS             2731..3918
                     /locus_tag="VFU90_14220"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5480997.1"
                     /translation="MSEAAVVSAARTPIGRAFRGAYNQTPGATLAGHVVRHAIERAGV
                     DPADVDEVVIGSGLPEGATGNNIGRTAALRAGCPVGVAGQTVSRYCASGLDAIVAAAQ
                     RVRSGDARVVVAGGVESVSMVQSHLNLEFYTEEWLLRHTPDVYMPMLYTADNVAKKYG
                     VSRERQDEYAFQSQQRTAAAQTAGRFDAEIVALPTWKYAQDRETGAVTEQHVLLAKDE
                     GNRPETTLEALQKLAGAVPGIKDTTITAGNSSQLSDGAAAVVVMDAESARDRGLEPLG
                     MYRGFAVAGCEPGEMGIGPVFAVPKLLARHGLTVDDIGLWELNEAFAVQTVYCRDTLG
                     IPNERFNVDGGAISIGHPYGMSGTRMAMHALIEGKRRGVKYVVVTMCVGGGMGAAGLF
                     EVV"
BASE COUNT          707 a         1233 c         1410 g          586 t
ORIGIN      
        1 cgcgctgcaa gtgttcggcg ggatgggata catcgaggaa accggaatcg cgcagcagta
       61 tcgcgacgtg cggatcgtga cgatctacga aggcacgacc gggatacaag cgctcgacct
      121 gatcggacgc aagctgattc gcgacatggg tgcgacggcg accgccgtcg gcgccaagat
      181 cgccgccgtc gcgaaagaat gcgccggcag cgagaacgcc gacgtcaagg cgatcggagc
      241 ggcgctggcg gagagtctca aggcgctcaa cgaaacctcg caatggatcg ggatgaacgc
      301 gatggccgac ttgaacaaag cgttcgcctg cggcgttccg tacctgcgct tgtggggcgt
      361 agtcgcgggc ggctggcaga tggcgcgcgc cgcggcgatc gccgcccgga agatcgcggc
      421 cggagatccc gaagcggact tctaccaagc caagctcgcg acggcgaagt tctacgcgac
      481 ccacgtgctc tcgcagggcg cgtggttcgc gcggcagatt accgagggat ccgccgacgt
      541 gatgggcctg agtgaagagc agctcgatct cgatcgcaag atgacggcga acgtctagca
      601 tggaggtgcg agtccgacgg caggccggcg ccggcggcaa gaacgtcgcc gtcttgacgc
      661 tcgacaatcc tccggtgaac gcgctctcgt tcgccttctc ggagcggctg ctcgaggcga
      721 tcgcggaggc tgaaaaggat cccggcgttt cggcggtcgt gttcgccggg gccggcggaa
      781 tcttcagcgg cggcgccgac gtcaacgatt tcaacgccga accgtcgccg gatgcgaaaa
      841 ccgttcgcga cgcaatcgcg gcggtcgaac gcggcacgaa gacgtacgtt gccgcgatcg
      901 acggcaacgc gctcggcggc ggtttcgaac tggcgctggc gtgcgactat cgcgtcgcta
      961 cgaaggccgc caaggtcggg cttccggaga tcaagctcgg gcttattccc ggcgcgggcg
     1021 ggacgcagcg gcttccgcga gcgatcggcg cgcaagccgc gctcgagttc atgctcaagg
     1081 ggcgcagcca caccgccgcc gaggccgcgg cgctcggact tctcgacgaa gtcgtcgacg
     1141 gcgatgccgt cgaggcggca atcgtcctcg cgcaacgcga ggcgccgcaa cggcgccgcc
     1201 tctcgcagcg ccgggcggtc atcggcgccg gaatctcacc gcaagcagcg ccgttcgtgg
     1261 tatcgcaggc gcacaagatg gtgccgcccg aagatcgcgg cggattcgcg gcgcacaaac
     1321 taatcgacgc ggtcgaagcg gcggtcgaga tgccgttcga gttcggcatc gcgcgcgagg
     1381 cgcggctgtt cgaagaactc gcgcgctcgg cgccgtcgca tgccctgcgg catctcttct
     1441 ttgccgagcg ggagctgagc aagattcccg ggctgccgcc cgccgaaccg ctcgaaatcg
     1501 gcaacgccgg cgtggtcggc gcgggcacga tgggcagcgg catcgcgatc gccttcgcgc
     1561 aggtcggcat cccggtcacg gtgatcgatt cgaacgacga agccgtcgac aaggcgcggc
     1621 agaccgtgat ggggatgttc atgtatcagg tgcagaaggg acgcctgacg caagaggagg
     1681 cctggaaacg cgggcagtcg attacgttta ccgacgattg gagcgagctt gccggcgcgg
     1741 acgtcgtggt ggaggccgtt ttcgaagatc tcgccgtgaa gaaagacgtt ttcgccaaac
     1801 tcgacggcat cgtcaaaccc gaagcgatcc tggcaacgaa cacctcgacg ctcgacgtcg
     1861 acgcgatggc ggccgccacc ggacggcccg aaaaggtgct ggggctgcac ttcttcgtgc
     1921 cggcgaacat catgccgctg ctggagatcg tccggggcgc ggccacgtcg ccgcaaacga
     1981 ttgccaccgc gttccggctc gccaaacggc tgcgcaaaac ggcggtgctc tccggcaacg
     2041 cgtttggatt catcggcaat cgcatggtgt tcgactacgc tcgcgaggcg ctggcactcg
     2101 ccgaagaagg cacgccgccg gcacgcatcg acggggtgat gaaggatttc gggttcccga
     2161 tggggccgtt tgcgatgtcg gatctttccg gcatcgacgt cgcctggcac atcgcgcaag
     2221 cgcgcggcgg tgagggtctg ggacggacgc aggtcgtgca gcggctggtc gaaagcaaac
     2281 ggctggggca aaaaacgatg gccgggtact tcaaatacga taagtcggtc ggcaaaggcc
     2341 gcgaaccgat ccccgacgcg gaagtcgaag cgctgttcgc agaggaagca aagcgcgcgg
     2401 gaatcgcacc gcgcgccgcc ggcgacgaag aaattcgcga gcgcttgctg tacgcgctga
     2461 tcaaccgggg cgcgtatctg ctggaagaag gcgtcgcgct gcggcccggc gacatcgacg
     2521 tcgtctacgt ttacggctac ggcttcccgc cgcatcgcgg cggtccgatg tggtacgccg
     2581 acgagaccgg ggtcgagcgc gtgtacgcga ccgtcgagcg cttcgcgcgc gatttcggcc
     2641 cgcagtggga accggcgccg ctgctcgccg ctatcgccgc ccggggcgga acattcgcgg
     2701 cgcacgcggc cgccgaaaag gagccggccg gtgtctgagg cggccgtcgt ttcggcggcg
     2761 cgcacgccga tcggacgggc cttccgcggc gcgtacaatc aaacgccggg cgcgacgctg
     2821 gcgggacacg tcgtgcgcca tgcgatcgag cgcgccggtg tggacccggc ggacgtggac
     2881 gaagtcgtca tcggttcggg gttgcccgaa ggtgcgaccg gaaacaacat cgggcgcacc
     2941 gcggcgctgc gggccggctg cccggtcggc gttgcgggac aaacggtcag ccggtactgc
     3001 gcgtccggtc tggacgcgat cgtggcggcg gcgcaacgcg tgcgcagcgg cgacgcgcgc
     3061 gtcgtcgtgg ccggcggcgt cgaatcggtg agcatggtgc agagccatct caacttggaa
     3121 ttctacaccg aagaatggct gctgcggcac acccccgacg tctacatgcc gatgttgtat
     3181 accgcggata acgtcgcaaa aaagtacggt gtgtcgcgcg agcgtcaaga cgaatacgcg
     3241 tttcagagcc agcagcgtac cgctgcggcg caaacggccg gccgattcga cgcggaaatc
     3301 gtggcgctgc ccacctggaa gtacgcgcaa gaccgggaaa ccggagccgt gaccgagcag
     3361 cacgtcctgc tcgccaaaga cgaaggcaac cggccggaaa cgaccctcga ggcgctgcag
     3421 aaactggcgg gcgcggttcc cggcatcaaa gacacgacga tcacggccgg gaacagctcg
     3481 cagctttccg acggcgcggc ggcggtggtc gtgatggacg ccgagtcggc gcgcgaccgc
     3541 gggctcgaac cgctggggat gtatcgcggc tttgcggtgg ccggctgcga gcccggcgag
     3601 atgggcatcg gcccggtctt cgccgtgccg aagctgctcg cacgccacgg tttgacggtc
     3661 gacgatatcg gcttgtggga gctcaacgaa gcctttgcgg tgcagaccgt ctactgccgc
     3721 gacacgctcg gcattcccaa cgagcgcttc aacgtagacg gtggcgcgat ctcgatcggg
     3781 catccgtacg gcatgagcgg cacccgtatg gcgatgcacg cgctcatcga aggcaagcgc
     3841 cgcggcgtca agtacgtcgt cgtcacgatg tgcgtcggcg gcgggatggg cgccgccggg
     3901 ttgttcgaag tggtgtagca gggaggattt gccggc
//
LOCUS       DASUCJ010001219         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_685596, whole genome shotgun sequence.
ACCESSION   DASUCJ010001219 DASUCJ010000000
VERSION     DASUCJ010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685596"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFU90_14225"
     CDS             <1..557
                     /locus_tag="VFU90_14225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU5480998.1"
                     /translation="LGVLHYLGSIIEIEAVAFPASQAGKGTIRFNDAAGSMAKDSVFN
                     ATSVMRATTGIDTADYDLHVNVVGGGNIDGPSAGLAIFLALYSAITKTPLPQDVAITG
                     ELSIQGKVRAVGGIVEKLYAARQAGMRRVVVPKENAREVDASLAGVDVVAVTGVDQVL
                     RELHMHRRSTRRSPGPRRRAARRP"
     gene            554..>1154
                     /locus_tag="VFU90_14230"
     CDS             554..>1154
                     /locus_tag="VFU90_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5480999.1"
                     /translation="MTAQPLPSAIDRIPPHNLEAEMAVLGSVLVDKAMMAEVGQLIKP
                     GDFYAHVHETIYSVLLELFEHDEPIDKITVSEALRSRNALERVGGLAYVGALMDTVQS
                     AASARYYATIVHEKAALRALIGAGTQVTALGYEGEEDVAGALDRAQQLVYAIGEERSE
                     KEFKPVKSLLKDAFDSIDRLYHLRGDRTGLTSGFPDIDAM"
BASE COUNT          213 a          372 c          389 g          180 t
ORIGIN      
        1 ggttgggggt gctccactat ctcggcagca tcatcgaaat cgaagcggtc gcgttccccg
       61 cgtcgcaagc cgggaagggc accatccgat tcaacgatgc ggccggctcg atggccaagg
      121 attcggtgtt caacgccacc agcgtgatgc gggcgaccac cggcatcgat accgccgact
      181 acgacttgca cgtcaacgtg gtcggcggcg ggaacatcga cggaccgtcc gccggtctgg
      241 cgatcttcct cgcgctctac tcggcgatca ccaagacgcc gctgccgcag gacgtcgcga
      301 tcaccggcga gctctcgatt caggggaagg tgcgcgcggt gggcgggatc gtcgagaagc
      361 tctacgccgc ccgccaagcc gggatgcggc gcgtcgtcgt ccccaaggag aatgcccgcg
      421 aggtcgacgc ttcgctggcc ggcgtcgacg tggtggccgt gaccggggtc gaccaggtac
      481 tgcgggaatt gcatatgcac aggcgctcca caaggcgttc accgggtccc cggcgcagag
      541 cggctcgacg tccatgaccg cgcagccttt accttcggcg atcgatcgca ttccgccgca
      601 caatctggaa gcggagatgg cagttctcgg ctccgttctc gtcgacaaag cgatgatggc
      661 ggaggtcggg cagctgatca agcccggcga tttctacgcg cacgttcacg agacgatcta
      721 ctccgtgctc ctcgaactgt tcgagcacga cgagccgatc gacaagatca cggtcagcga
      781 agcgctgcgc agccgcaacg cgctcgagcg ggtcggcggc ctcgcgtacg tcggcgcgct
      841 gatggatacg gtgcaatcgg cggcgtcggc gcgttactac gcgacgatcg tccacgagaa
      901 agcggcgctg cgggcgctga tcggcgcggg cacgcaggtg acggcgctcg gctacgaagg
      961 cgaagaagac gtcgccggcg cgctcgaccg ggcgcagcag ctcgtctacg cgatcggtga
     1021 agagcgcagc gaaaaagagt tcaagccggt caagagcctg ctcaaagacg cgttcgacag
     1081 catcgaccgc ctctatcatt tgcggggcga ccggaccggc ctcacgtcgg gcttccccga
     1141 catagacgcg atga
//
LOCUS       DASUCJ010001220         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1235883, whole genome shotgun sequence.
ACCESSION   DASUCJ010001220 DASUCJ010000000
VERSION     DASUCJ010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235883"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFU90_14235"
     CDS             complement(<1..821)
                     /locus_tag="VFU90_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481000.1"
                     /translation="MLTRRRALFRLLAAMVAVNAGCDFGGSNTPATEPDLTLDGRSDA
                     ATAHDAARDALADAPSETRPADGAIEAAVVTDAGATIDAAEAAAIDAGAIVDAASDVA
                     PPPAVEAGIDATVDAGIDAGVDAASVVVVDAADADARDAAPAPVAVTISGPTGPESGA
                     PVVFHDPSGTQLGVVTTDSTGAASMLVPPGSMVTAIFGNPNSSVLVSIAAVQPGDVIP
                     IVDPAPAISLVASPTIHLPDSAPPSGTTQYELVVGGSCGSTVSSSTSTVTVDVES"
     gene            complement(870..>1556)
                     /locus_tag="VFU90_14240"
     CDS             complement(870..>1556)
                     /locus_tag="VFU90_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481001.1"
                     /translation="NGSIYRRDPTSGTQALMSIATGIPLTQWAGVDATLLPANIMAGL
                     SGTSAVVNALETDQNPEQAIAIFSATNLDVSKVSVLAYKDRDQACGYYPDSSPTARDK
                     ANVRDGHYALWGPIHFFTNVDSSGTPVNHNVQRILNYITGTLPAPDGLDLVQIEALAN
                     LVPECAMRVKRSVEMGPLVSFAVPSCGCYFDYVATGQSSCQPCAKQSDCPATAPVCNT
                     NYPKAFCETQ"
BASE COUNT          263 a          493 c          549 g          251 t
ORIGIN      
        1 ttggactcga cgtctacggt aacggtcgat gtagaggagc tcaccgtcga cccgcaagag
       61 ccgccgacga cgagctcgta ctgggttgtc cccgaaggag gagcagagtc cggcagatgg
      121 atcgtcgggc tcgcgacaag cgagatggcc ggcgcgggat cgacgatggg aatcacgtcg
      181 cccggctgca ccgccgcgat gctgacgagg acggagctat tcggatttcc gaagatcgcc
      241 gtcaccatcg atccgggggg caccaacatg gaggccgcgc ccgtcgaatc ggtggtcacg
      301 accccgagct gggtgcccga cggatcgtgg aagacgaccg gcgcgcccga ctcgggtccc
      361 gtcggaccgg agatggtcac cgcgaccggc gcgggcgcgg cgtcgcgcgc gtcggcgtcg
      421 gctgcgtcga cgacgaccac ggaagcagca tcgactccgg cgtcaatccc cgcgtcgaca
      481 gtcgcatcga tccccgcttc gacggcgggc gggggggcca cgtcagacgc ggcgtcgacg
      541 atggctcccg cgtcgatcgc cgccgcctcc gccgcgtcga tggtcgctcc tgcatcggtg
      601 acgaccgccg cttcgatcgc tccgtcggcg ggacgggttt cgctcggcgc atccgccaag
      661 gcatcgcgag ccgcgtcgtg ggcagtggct gcgtcggatc gcccgtcgag cgtgagatcc
      721 ggctcggtcg cgggagtatt cgaaccgccg aaatcgcaac cggcgttcac cgccaccatt
      781 gccgccaaaa gccggaagag cgcgcgacgc cgcgtgagca tcggcggagt atcgaggtaa
      841 cccacgccgc ggtcaatcgc ggggccgacc tattgcgttt cgcagaacgc tttcgggtaa
      901 ttcgtgttgc acacgggcgc ggtcgcgggg cagtccgatt gcttcgcgca cggctggcag
      961 gacgactgac cggtggccac ataatcgaag tagcaaccgc acgaaggtac cgcgaacgag
     1021 acgagggggc ccatttcgac ggaccgcttg acgcgcatcg cgcactcggg gaccagattc
     1081 gcgagcgcct cgatctgcac gagatccagc ccgtccggtg cggggagggt acccgtgatg
     1141 tagttcagga tccgctgcac gttatggttc accggggtgc cgctcgagtc cacgttcgtg
     1201 aagaagtgga tcggtcccca gagcgcgtag tgtccgtcgc gcacgttcgc cttgtcgcgt
     1261 gccgtcggcg acgaatcggg atagtacccg caggcctggt cgcggtcttt gtaggcgagg
     1321 acggagactt tgctcacgtc gaggttcgta gcggagaaga tcgcaatcgc ctgttcggga
     1381 ttctgatcgg tttccagcgc gttcacgacg gcggaagtac cggagagccc cgccatgatg
     1441 ttcgcgggca ggagcgtcgc atcgacaccg gcccactgcg tcagcgggat cccggtggcg
     1501 atggacatga gcgcttgcgt tcccgaggta gggtcacggc ggtaaatcga gccgtt
//
LOCUS       DASUCJ010001221         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_578826, whole genome shotgun sequence.
ACCESSION   DASUCJ010001221 DASUCJ010000000
VERSION     DASUCJ010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578826"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1508)
                     /locus_tag="VFU90_14245"
     CDS             complement(<1..>1508)
                     /locus_tag="VFU90_14245"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481002.1"
                     /translation="VAGAMMKGIGVDKVKSAKGEAIEQKSMNSLFIMADSGARGSAAQ
                     IRQLAGMRGLMARPDGSIIETPIKANFREGLDVLQYFNSTHGARKGLADTALKTANSG
                     YLTRRLVDVAQDVVVTEPDCGTLAGLTMTAIVEGGDVVEPLRDRVLGRIVAADVYAPG
                     NDNKPIITRNTLLDEKLVEKLDAAGVQSILVRSPISCESSFGVCAHCYGRDLARGHLV
                     NMGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAVDNVQVKTVGTIKFNNLKTVH
                     HAQGHLVAVSRSGELSVMDAHGRERERYKVPYGATISLKDGGAVKAGQVVANWDPHTH
                     PIVSEVAGKVRFIDFIDGVTVQQQTDELTGLESNVVTDPKRRGTQAKDLRPTVRLEDK
                     KGKELKLPGTDIPAQYFLPAGAIVSLQDGAEVGVGDVVARIPQETSKTRDITGGLPRV
                     AELFEARRPKDHAIIAEISGVIRFGKDFKNKQRVSIEPAEEGAEAVEYLIPKS"
BASE COUNT          239 a          504 c          487 g          278 t
ORIGIN      
        1 gacttcggga tcagatactc gaccgcctcg gcgccctctt ccgcgggctc gatgctgacg
       61 cgctgcttgt tcttgaagtc cttgccgaag cggatgacgc ccgaaatctc cgcgatgatc
      121 gcgtgatcct tcggacggcg cgcctcgaac agctccgcca cgcgcggcag accgccggtg
      181 atgtcgcgcg tcttggaggt ttcctgcggg atacgcgcga cgacgtcgcc gacgccgacc
      241 tcggcgccgt cctgcagcga cacgatcgcg ccggccggca ggaagtactg cgccggaatg
      301 tcggtgccgg gcaatttcag ttccttgccc ttcttgtctt ccaggcgcac ggtcgggcgc
      361 agatccttgg cctgcgtacc gcggcgcttc ggatcggtga cgacgttgga ttcaagcccg
      421 gtgagttcgt cggtctgctg ctgcaccgtc acgccgtcga tgaaatcgat gaagcggacc
      481 ttgcccgcca cttcggaaac gatcggatgg gtgtgcggat cccagttcgc cacgacctgg
      541 ccggccttga ccgccccacc gtccttgagc gaaatcgtcg cgccgtacgg caccttgtag
      601 cgctcgcgct cacggccgtg cgcgtccatc acggacagct cgcccgaacg cgacacggcg
      661 acgagatggc cctgcgcatg atgcacggtc ttgaggttgt tgaacttgat cgtaccgacg
      721 gtcttgacct gcacgttgtc gaccgcggcc gcacgcgagg ccgcgccgcc gatgtggaac
      781 gtacgcatgg tcagctgcgt gccgggctcg ccgatggact gggcggcgat gacgccgacc
      841 gcttcgccca tgttgacgag atggccgcgc gcgaggtcgc gaccgtagca atgcgcgcag
      901 acgccgaaac tcgactcgca actgatcggc gaacgcacca ggatcgactg cacgccggcg
      961 gcatcgagct tctcgaccaa tttttcatcg agcagcgtat tgcgcgtgat gatcggcttg
     1021 ttgtcgttgc ccggtgcgta cacgtcggca gccacgatgc ggccgagcac gcggtcgcgc
     1081 aacggctcga ccacgtcgcc gccttcgaca attgccgtca tggtcaggcc cgccagagta
     1141 ccgcagtccg gttcggtcac gaccacgtcc tgggccacgt ccacgagacg acgggtgagg
     1201 taacccgagt tggcggtctt cagcgcggta tcggccagac ccttgcgcgc gccgtgcgtg
     1261 gagttgaagt actgcagcac gtcgagacct tcgcggaagt tcgccttgat cggcgtttcg
     1321 atgatcgaac cgtccggacg cgccatgagg ccgcgcatgc cggcgagctg gcggatctgc
     1381 gctgcggaac cgcgcgcgcc ggaatcggcc atgatgaaca gcgagttcat cgacttctgc
     1441 tcgatggcct cgcccttggc ggacttgacc ttgtcgacgc cgatgccctt catcatcgcg
     1501 ccggcgac
//
LOCUS       DASUCJ010001222         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1526105, whole genome shotgun sequence.
ACCESSION   DASUCJ010001222 DASUCJ010000000
VERSION     DASUCJ010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526105"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFU90_14250"
     CDS             complement(<1..330)
                     /locus_tag="VFU90_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU5481003.1"
                     /translation="MTPRTVRHQDEAADYSSYLALDELLSLQRPLSEPEHPDELLFIV
                     VHQASELWFKVILHELDALVHALESATVAAAMMVAKRLNALVRIVADQLSALDTLPPQ
                     RFAQFRGY"
     gene            complement(327..1046)
                     /gene="bshB1"
                     /locus_tag="VFU90_14255"
     CDS             complement(327..1046)
                     /gene="bshB1"
                     /locus_tag="VFU90_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU5481004.1"
                     /translation="MTHVDVLAIAAHRDDIELTCGGTLLKAQSLGHATAIIDLSQGET
                     GTRGSAELRAQEASRAAEILGVSARVNLGFPDAAIVNTPATRIAVAKAIRQFTPRIVI
                     APSERGRHPDHRVTAELVRDACFIAGLAKVEPGVPAHRPHKVIHCLSFREDYIKPTFV
                     VDISDVFEQKLEAVRQYESQFAGVHQAGEVYPNGEPLTEIVRHQAAHYGSLIRVRYGE
                     PFSTAETMRVDDVTTLEVSTY"
     gene            1214..1624
                     /locus_tag="VFU90_14260"
     CDS             1214..1624
                     /locus_tag="VFU90_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5481005.1"
                     /translation="MRAFHLVSLGIAAFALACGGSGSSSMNGVTDPPPPMNGVNVTIA
                     DFKFSPAVDTVKVGTTVQWVNHGPSEHSTTSDTGLWNSGGLVPSGGTGSYAASTGGST
                     FQFTFTQAGSFSYHCMFHPPSQYPSFVGTIVVMP"
     gene            complement(1854..2891)
                     /locus_tag="VFU90_14265"
     CDS             complement(1854..2891)
                     /locus_tag="VFU90_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481006.1"
                     /translation="MRVGAVSAGVLLAALAACTHNTQRDNLSPRDEPLDVSLIPGLQA
                     ETTVPSLSFVLNRPAYVAAFEVIPGRGMQLLYPAGAADEESNAGLNVVTEAPFFYADY
                     LLPPFVAPAAQPHYIYIVASDAPLNLSTVREPAGLVHMFGIERFASATPSYLMDQVTS
                     QVIEPGTSDDAWASDMVMDWSGAGGVDLTLATTQIRCRDGSLIIVPLSYGSVLCPGDA
                     LRAHPVLAAYRAPTNRKPSKHPSKPSTKASSQTLAAQRAAHHAPRWGSDAPQVTGGLL
                     GWRLVHLPADRPTAAPKVQSANPQHPPDPRPATSAPAHGAPPAAAPAPARTSAPPPAA
                     APPAQKIHPSR"
BASE COUNT          519 a          998 c         1091 g          548 t
ORIGIN      
        1 gtagccgcga aactgcgcga agcgctgcgg cggcagcgtg tcgagcgccg acagttggtc
       61 ggcgacgatg cggacgagcg cgttgagccg cttggccacc atcatcgcgg ccgccacggt
      121 ggccgactcg agcgcgtgca cgagcgcgtc gagctcgtgc aggatcacct tgaaccacag
      181 ctcgctcgcc tggtgcacga cgatgaagag gagctcatcg gggtgctcgg gctccgacaa
      241 cggccgctgc agcgacagca gctcgtccag cgcgaggtag ctgctgtagt cggccgcttc
      301 gtcttggtgc ctaacggtgc gcggcgtcaa taggtggata cctccagcgt ggtcacgtcg
      361 tcgacgcgca tggtctcggc ggtggagaac ggctcgccgt accgcacgcg gatgagcgat
      421 ccgtagtggg ccgcctgatg cctgacgatt tcggtcaacg gttcgccgtt cgggtagacc
      481 tcgcccgctt ggtgcacgcc ggcgaactgc gattcgtact ggcgcaccgc ctcgagcttc
      541 tgctcgaaca catcggagat gtcgacgacg aacgtgggct tgatgtagtc ctctcgaaac
      601 gagaggcagt gaatgacctt gtgcggacgg tgcgccggca cacccggctc gactttggcg
      661 agtccggcga tgaaacacgc atcgcgcacc agctcggccg tcacacggtg gtccgggtga
      721 cggccgcgct ccgatggcgc gatgacgatg cgcggtgtga actggcggat cgctttcgcg
      781 acggcgatgc gcgtggccgg tgtgttgacg atcgccgcgt cggggaatcc gagattgacg
      841 cgcgcgctca caccgagaat ttccgcggcg cggctggctt cttgtgcgcg cagctccgcc
      901 gagccacggg tgccggtctc gccctgcgac agatcgatga tcgccgtcgc gtggccgagt
      961 gactgtgcct tgaggagtgt gccgccacag gtgagctcga tgtcatcgcg gtgcgcggcg
     1021 atggcgagca catcgacgtg tgtcatggcg cagaatatat cgcgcgtcga gccccaagcg
     1081 agtcgatgag gtcgtcagtc gcggaaccat ctcccgggcc cgcgaatatg cctttcgttg
     1141 gtccgaagga aacgcggcgc tgtcgagcac aaccgctcgc gcggcgtgcg ttcctcacag
     1201 cagaggaggc caggtgcgtg catttcatct cgtcagtctc gggattgcag ccttcgcgct
     1261 cgcgtgcggt ggcagcggca gcagttcgat gaacggcgtc acggatccgc cgccaccgat
     1321 gaacggtgtg aacgtcacga ttgccgactt caagttctcg ccggcggtgg acacggtgaa
     1381 agtggggacg acggtgcaat gggtgaatca cggtccatcg gagcacagca cgacgagcga
     1441 caccggtttg tggaactcgg gcggacttgt cccatccggc gggaccggtt cgtacgccgc
     1501 gagcacgggc ggttcgacgt tccagttcac gttcacgcag gcggggtcgt tcagctatca
     1561 ctgcatgttc catccgccgt cgcagtatcc gtcgttcgtc gggacgatcg tcgtcatgcc
     1621 gtgacggtcg ccggcgccga tgcctaacgg ccagccggtg cgatcgagcg ccggctggcc
     1681 gtctcaggct cgcgcgaacg acacgccgct ctgcgggcga ttcgtgatgc gccgaacgac
     1741 gtcgagtcgg tgggctgcgg ggcaacgtcc ttgccacggg tcgagaattt gcctcgagcc
     1801 accgatccgc cgcgtcgtct ccgcctgtca tcatatcatc tgatgagtcg gcgtcagcgt
     1861 gaaggatgga tcttctgcgc tggtggtgcc gctgcaggcg gaggcgccga tgtgcgcgcc
     1921 ggagccgggg cggcggccgg cggtgcgcca tgagcgggcg cggacgttgc cggccgtgga
     1981 tccgggggat gctgcggatt cgccgactgc accttgggcg cggcggttgg gcggtcggcc
     2041 ggcaggtgca ccaagcgcca gccaagcaaa ccacccgtta cctgcggtgc gtcgctcccc
     2101 cacctcggtg cgtggtgtgc cgcccgctgg gccgcgagcg tctggctcga agccttggtt
     2161 gacggcttgc tcggatgctt cgatggtttg cggttcgtcg gcgcgcggta cgcggccaag
     2221 accggatgcg cgcgtaacgc gtcgcccggg cagagtaccg acccgtacga caatggtacg
     2281 atgatcagac tgccatctcg gcatcgaatc tgagtcgtag ccaacgtgag atccaccccg
     2341 ccggcgcctg accaatccat taccatgtcg gacgcccacg catcgtccga cgtcccaggc
     2401 tcgatcacct gcgacgtgac ctgatccatc aaataagacg gcgtcgccga cgcaaatcgc
     2461 tcgatgccga acatgtgcac caagccggcg ggttcgcgca ccgtactcag gttgaggggc
     2521 gcgtcggacg caacgatgta gatgtagtgc ggttgcgctg cgggcgccac gaacggcggc
     2581 agtagataat cggcgtagaa gaacggcgct tcagtgacga cgttcaagcc ggcgttgctc
     2641 tcttcgtctg cggcgcctgc gggatacaga agctgcatgc cccggcccgg gatgacctcg
     2701 aatgccgcaa cgtacgccgg ccgattgagc acgaaggaga gcgatggaac ggtcgtctcc
     2761 gcttgcaggc cggggatcag gctgacgtcc agtggctcat cccgcgggga gaggttgtcg
     2821 cgctgggtgt tgtgggtgca ggcggcgagc gccgccaaca gaacaccggc cgacaccgcg
     2881 ccgactcgca tacgccgcct cgcctaaacg tgtcgctaac tctacgcgcg ggcgctccgg
     2941 aacgttccgc gatcgttaga cacgcggaac gcagtgggtt ggccggtcat tcgtaccgca
     3001 atgcctccac cggatcgagc cgcgccgccc ggttggccgg cagcaacccg aataccacgc
     3061 cgatggcgac cgacacgacc accgccacga gcgtgtagag catcggggtg gttgcctcga
     3121 tccgcatggt cgccgtggcg attcgtccga atccta
//
LOCUS       DASUCJ010001223         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_563261, whole genome shotgun sequence.
ACCESSION   DASUCJ010001223 DASUCJ010000000
VERSION     DASUCJ010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563261"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFU90_14270"
     CDS             complement(<1..1024)
                     /locus_tag="VFU90_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5481007.1"
                     /translation="MSTPTLVVLEGDQTGQELLLEALRLLDRSVIDLELAFERYDLSL
                     ENRRATGNGVVFEAAAALKNHGLGLKAATITPEEKDGVGSPNRILREQVDGKVIVRTG
                     RKLPRVRPVAGIHAPISVVRMAVGDAYGAQEWREGEGMDEVAHRTETIGRGVCRYVAE
                     FAFVHARKTGGVVFGGPKYTVSPVYEGMLKEEMDAAAARHPDVAYDPQLIDATYALLL
                     SNAGDRPLVIPSLNRDGDCLSDLVMQLFGSIAGAESVLLAFGPLQPGESPPASGFAPS
                     VVMAEAPHGTAPRLQGKNVANPLAMILAAAAVLSYVSDRRAANASRAVYEAALEALHA
                     GIATADL"
     gene            1062..>3044
                     /locus_tag="VFU90_14275"
     CDS             1062..>3044
                     /locus_tag="VFU90_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU5481008.1"
                     /translation="MARARRKTTARRKINLEIVGIAAIAIAVLCAVSLAVPHHAGNFG
                     GWIADALRRLFGGAAPLFPVLVAFFGAIVFLEVNVPRTVAGPGTTALAYFLIVDAAFG
                     AGGPSRGGAVGANIWWALHALIGTVGAWIVIGVAALSLTLWLTGASVKKLIGRAIVFA
                     GGLRMPQLPKLPSLRAGFPEGHGSLRDAFALPAQRAPTRAPFDDTATEVEVEVERVPA
                     PYPAEALVPIVPAPRPVEPERPRPERPAALEEPAGGPRAYRLPDLAIFDPPPAQVVDE
                     SNRAHVLEDTLASFGVGAKVVHIERGPSITRYELKPERGVKISRIASLADDLALAMAA
                     TSVRIEAPIPGKSAVGIEVPNQSVAIVAIREILDAIPNRGQIPPLWMALGKDITGRPV
                     FGDLCKMPHLLVAGATGAGKSVCLNTIIASLLVSATPDQVQLLMIDPKRVELTVYNGI
                     PHLIKDVIADPRLAAGALFEMTKEMDSRYERFAKSGVRKIEEYTAKHPDETLPYVVIV
                     IDELADLMLVAPAKVETTIMRLAQLARATGIHLVVATQRPSVDVITGLIKANIPSRIA
                     FAVSSQADSRVVLDMNGAERLLGRGDMLYLPIDAPKPVRAQGALISGGEVARLVEFWA
                     QQARPHNLLDVEVVPLSDDEPGKAVDPLCYDAAKFII"
BASE COUNT          478 a         1042 c         1020 g          504 t
ORIGIN      
        1 cgaggtcggc cgtggcgatt ccggcgtgca gcgcctcgag cgccgcttcg tagacggcgc
       61 gcgacgcgtt agcggcgcgc cggtcgctga cgtacgagag cacggcggcg gcggcgagaa
      121 tcatggccag cggattggcg acgtttttcc cctgcagccg cggcgcggtg ccgtgcggcg
      181 cttcggccat cacgaccgac ggcgcgaatc ccgaagcggg cggactctcc cccggctgca
      241 gcggcccgaa cgccagcagc accgattcgg cgccggcgat cgaaccgaat agctgcatca
      301 ccagatcgga aagacaatcg ccgtcgcggt tcagcgaagg aatcaccagc ggccggtcgc
      361 cggcgttcga aagcagcagc gcgtaggtcg cgtcgatgag ctgcggatcg taagcgacgt
      421 cgggatgacg cgcggcggcg gcgtccattt cctctttgag cattccctcg tacaccgggc
      481 tcacggtgta cttcggcccc ccgaacacca cgccgccggt cttacgcgcg tgtacgaagg
      541 cgaactcggc gacgtaccgg caaacgccgc ggccgatcgt ttcggtgcgg tgcgccacct
      601 cgtccatgcc ttcgccctcg cgccattcct gggcgccgta cgcgtcgccg acggccatgc
      661 gaacgaccga aatcggcgcg tgaatgccgg cgacggggcg cacgcgcggc agtttccgtc
      721 cggtgcgcac gatgacttta ccgtcgacct gctcgcgcag aattcgattg ggcgaaccga
      781 cgccgtcttt ttcctccggc gtgatggtcg cggcctttag gcctagtccg tgattcttca
      841 gcgcggccgc cgcttcgaac acgacgccgt tgccggtcgc gcgccggttt tcgagcgaga
      901 ggtcgtaacg ttcgaacgca agctcgaggt cgatgacgga acggtcgagc agccgcagcg
      961 cttccagcag cagctcctgt ccggtctggt cgccttcgag gacgacgagg gtcggcgtgc
     1021 tcaaaatgaa ctcctggtgt tacgacgaaa cgatatattc catggcacgc gcgcgccgca
     1081 agacgacggc gaggaggaag atcaatctcg agatcgtcgg gattgccgcc atcgcgatcg
     1141 ccgtcctctg cgccgtctca ctggcggtgc cgcaccatgc cggcaatttc ggcgggtgga
     1201 tcgccgacgc gctgcgccga ctctttggag gcgccgcccc gcttttcccg gtgctcgtgg
     1261 cgttcttcgg cgcaatcgtt tttttggaag tcaacgtgcc gcggacggtc gccggacccg
     1321 gcaccaccgc gcttgcgtac tttctgatcg tcgatgccgc cttcggcgcg ggcggtccga
     1381 gccgcggcgg cgcggtcggg gcgaacattt ggtgggcgct gcacgcgctg atcggcaccg
     1441 tcggcgcctg gatcgtcatc ggcgtcgcgg cgctgtcgct caccctgtgg ctgaccggtg
     1501 cgagcgtgaa aaagctgatc gggcgtgcga tcgtttttgc cggcggtctt cgcatgccgc
     1561 agctcccgaa gctgccgtcg ctgcgcgccg gcttccccga agggcacgga tcgctgcgcg
     1621 acgcgttcgc gctccccgcc caacgcgcgc cgacgcgcgc acccttcgac gataccgcga
     1681 ccgaggttga ggtcgaggtc gaacgcgtgc ccgcgcccta tccggccgag gcgctggtgc
     1741 cgatcgtccc cgccccccgg ccggtcgagc cggagcggcc gcgccccgaa cgacccgcgg
     1801 cgctcgagga accggccggc ggtccgcgcg cctaccggct gcccgatctc gccatcttcg
     1861 atccgccgcc ggcgcaagtg gtcgacgaat cgaaccgcgc gcacgtgctc gaggatacgc
     1921 tggcaagctt cggcgtcggc gccaaagtgg tgcacatcga gcgcggccct tcgatcaccc
     1981 gttacgagct caagcccgag cgcggcgtaa agatttcgcg gatcgcctcg ctggccgacg
     2041 atctggcgct cgcgatggcg gccaccagcg tgcggatcga ggcaccgatt ccggggaagt
     2101 cggcggtcgg catcgaggtg ccgaaccagt cggtggcgat cgtcgcgatc cgcgagattc
     2161 tcgacgcgat ccccaaccgc ggccagattc cgccgctgtg gatggcgctc ggcaaagaca
     2221 tcaccggccg tccggtgttc ggcgatctct gcaagatgcc gcacttgctg gtggcgggcg
     2281 cgaccggtgc cgggaaatcg gtatgcctca acacgatcat cgcgtcgctg ctggtcagcg
     2341 ccacgccgga tcaggtgcag ttgctgatga tcgaccccaa gcgcgtggag ctgaccgtct
     2401 acaacggcat cccgcacctg atcaaggacg taatcgcgga tccgcggctg gcggccggcg
     2461 cgctgttcga gatgaccaag gaaatggatt cgcgttacga acgctttgcg aaatcgggcg
     2521 tgcgcaagat cgaggaatac accgccaagc atcccgacga gacgctgccc tacgtcgtca
     2581 tcgtcatcga cgagctcgcc gatctcatgc tggtggcgcc cgcgaaagtg gagacgacga
     2641 tcatgcggct ggcgcagctc gcgcgcgcga ccggaatcca cctggtcgtc gcgacgcagc
     2701 gtccctcggt cgacgtgatc accggtttga tcaaagccaa cattccgtcc cgcatcgcct
     2761 tcgccgtaag ctcgcaagcc gactcgcgcg tcgtgctcga catgaacggc gccgagcggc
     2821 tgctgggccg cggcgacatg ctgtacctgc cgatcgacgc ccccaagccg gtgcgcgcgc
     2881 aaggcgcgtt gatctccggc ggcgaggtcg cgcggctggt ggagttctgg gcgcagcagg
     2941 cgcggccgca caacctgctc gacgtcgagg tcgttccgtt gagcgacgac gaacccggca
     3001 aagccgtcga tccgttgtgc tacgacgccg ccaagttcat catc
//
LOCUS       DASUCJ010001224         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1067714, whole genome shotgun sequence.
ACCESSION   DASUCJ010001224 DASUCJ010000000
VERSION     DASUCJ010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067714"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFU90_14280"
     CDS             complement(<1..210)
                     /locus_tag="VFU90_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5481009.1"
                     /translation="MRSSRKLALVTGASGGLGAEFAALLAADGYDLVLVARSADRLEN
                     RAAELRNRYGVTVTPVAMDLAKSDAA"
     gene            complement(194..1159)
                     /locus_tag="VFU90_14285"
     CDS             complement(194..1159)
                     /locus_tag="VFU90_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5481010.1"
                     /translation="MRTAIGVDLGGSHVTAAVVTDDGRIHSQHEEDLASLKFEAVVDA
                     LAAQLQAAIKDAGSGLVGIGVGSPGNIDAVSGAILYSPNFGWSDAPLGEALRKKFSLP
                     VFVGNDARCATLGEYEYGIGKGTKDFVLLTLGTGIGGGIVAGGRLILGNRWGAGEIGH
                     HQIRPTDGFVCACGKIGCFEAQASGTGLIRHAFAVAPSFPRSTLLDVARDKLSSKKIR
                     KAAQAGDNHALAAWKNFTADLAIGLANVIAFVNPETIALGGGVSTAGDFMLDAVRDPV
                     DALTTMVPRGTTRITIAQLGNDAGQVGAATMAFRGGLSTGDAFVP"
     gene            1222..>1645
                     /locus_tag="VFU90_14290"
     CDS             1222..>1645
                     /locus_tag="VFU90_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481011.1"
                     /translation="METIDLINLRNFARRTGQPTHPELLDARPIEHVNKIEIDEEHVT
                     IEFDEKTTRYYNTNEITKREWTYKYNDDPEFVKAVGKVIDLARKHVKDMPANVACPPG
                     CAECCSGYEPFVSHADVQRIADHLGMTYDAVMRDYVVAR"
BASE COUNT          307 a          568 c          501 g          269 t
ORIGIN      
        1 cgccgcatcg gatttggcca aatccatcgc aaccggcgtg accgtcaccc cgtagcgatt
       61 gcgcagctcc gccgcccggt tttcgaggcg atccgccgag cgcgccacca ggacgagatc
      121 gtagccgtcg gccgccagca gcgcggcgaa ctccgcgccg agaccgcccg atgcgccggt
      181 aaccagcgcg agcttacggg acgaacgcat cgccggtaga caagccgccg cggaacgcca
      241 tggtcgcggc cccgacctgt ccggcgtcgt tgccgagctg cgcgatcgta atccgcgtcg
      301 tgccgcgcgg caccatcgtc gtgagcgcgt ccaccgggtc gcgcacggcg tcgagcatga
      361 agtcgcctgc ggtcgagacg cctccgccga gcgcgatcgt ctccgggttg acgaaagcga
      421 tgacgttggc caggccgatc gcgagatcgg ccgtgaaatt cttccaggcc gccagcgcgt
      481 ggttgtcgcc ggcctgcgcg gctttgcgga tttttttaga gctgagctta tcgcgtgcga
      541 cgtccagcag cgtgctgcgc ggaaacgacg gcgccaccgc aaacgcgtgg cgaatcaacc
      601 cggttccgga cgcttgcgct tcgaagcagc cgatttttcc gcacgcgcac acgaatccgt
      661 cggtcggcct gatctgatgg tgcccgatct cacccgcgcc ccagcggttg ccgagaatca
      721 atcgtccgcc ggccacgatc ccgcccccga tcccggtccc gagcgtcaac agaacgaagt
      781 ccttcgttcc ctttccgatg ccgtattcgt attcgcccag cgtggcgcag cgcgcgtcgt
      841 tgccgacgaa caccggcagg ctgaacttct tacggagcgc ttcaccgagc ggcgcgtcgc
      901 tccagccgaa gttcgggctg tacaggatcg cgccgctgac ggcgtcgatg ttgccgggcg
      961 agccgacgcc gatccctacc aaaccggagc cggcatcctt gatggccgct tgcagttgag
     1021 ccgcgagcgc gtcgacgacc gcctcgaact tcaggcttgc gaggtcctcc tcgtgctggg
     1081 aatgaatcct cccgtcatcc gtaacgactg ccgccgtaac gtgagatccg ccgagatcga
     1141 cgccgattgc cgttcgcatg gagcttatgt ttggcgcggt cacaggccgt acccgcacgc
     1201 cgggggaatc gaagtagacc catggagacc atcgacctca tcaacttgcg caatttcgcg
     1261 cgccgtaccg gtcagcccac gcaccccgag ctgctcgacg cacggcccat cgagcacgtc
     1321 aacaagatcg aaatcgacga ggagcacgtc accatcgagt tcgacgagaa gacgacgcgc
     1381 tattacaata ccaacgaaat tacgaagcgg gagtggacgt acaagtacaa cgacgatccc
     1441 gaattcgtca aagccgtcgg taaagtcatc gatctcgcac gcaagcacgt gaaggacatg
     1501 ccggcaaacg tcgcctgccc gccgggctgc gccgaatgct gcagcggcta cgaaccgttc
     1561 gtcagccacg ccgacgtgca gcggatcgcg gaccatctcg gcatgaccta cgatgccgtc
     1621 atgcgcgact acgtggtcgc gcgac
//
LOCUS       DASUCJ010001225         9243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_28373, whole genome shotgun sequence.
ACCESSION   DASUCJ010001225 DASUCJ010000000
VERSION     DASUCJ010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28373"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFU90_14295"
     CDS             <1..1677
                     /locus_tag="VFU90_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5481012.1"
                     /translation="DLEVVEFDLLSQIEGIGGMLVPQAHAKQIGLMTYVDPDIPFRLL
                     GDPVRLRQVLVNLAGNAIKFTSKGCVAVSADLMSAGAQHVSIRFSVQDTGIGIEPGIL
                     PSLFEAFTQADGSTTRRYGGTGLGLAISKQLVELMGGSIEVESQPGAGSTFSFTLEMR
                     VGSNVRRLPARAEVRGTRVLIVDDDKMSRDILSRYVTAWGMIAKTAQGAGEALEMLTR
                     SAEGPERFHIAIVDLRMAGVDGIRLGYAIRRNRAIAQTKLVLVTAYDAPTVGQAAIRA
                     GFSCYLTKPIRHSQLYDAISDTLLGSVPVTIKPAVRAVEAAHRDTILLAEDNAVNRTV
                     SLQQLKKLGYTVDAVANGKEAVERVAGGTYGLVFMDCRMPVMDGFEATRAIRKIESRT
                     GRRVPIVAMTANALSNDRDDCLAAGMDDYLTKPVGLDDLRAVLKRWLGESEESTVIDS
                     DRISGIFGGDRDAVVEFFASVVPSIGALCEKIFETSDVAELRELVHELKGAASNIGAR
                     ELAIAAADLERGLAGGSDNQAALQILLTKLEQSWERFREAMDNQPEASFG"
     gene            1679..2758
                     /locus_tag="VFU90_14300"
     CDS             1679..2758
                     /locus_tag="VFU90_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5481013.1"
                     /translation="MIVLAVDDSDINLAVYRGMLKQIPDVQVVCHTSPLDALAWAAGN
                     SADVVAVDYHMPELDGLRFVERFRTLARSAHALIVIVTADTEIGVRHRALEMGANDFL
                     TKPIDRIDFTARIRNLLEIARSRRQLADRAEWLREEVERATASLAAREIETIVRLTRA
                     AETRDDVTGMHVIRVGHICAALARTVGLPDDECKTLLLAAPMHDIGKVATPDSILFKP
                     GPLTEREGAVMRNHTIAGYEILKDSESEMLQCAAEIALTHHECFDGSGYPRGLKGTAI
                     PLAGRLCSVADVFDALTSIRPYKLAWSVEKAVEMLDEGCGTQFDPEIVAAFHRALDDI
                     LGIKHRFADQGAATDKLARAYGHFQ"
     gene            2810..3562
                     /locus_tag="VFU90_14305"
     CDS             2810..3562
                     /locus_tag="VFU90_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481014.1"
                     /translation="MKTAPYGRIVFGASAVLSGVIALMWHDADTWQSLAKFWSLPFGL
                     IVGGCLMAAQIAGGVALAYPRSARAASIVLGIVYVLFSLICIPGIVAAPAVYVQYGAF
                     FEQFSLVCGALAVYAATEPDAARAAALGRAARIGMGLCAVSFTLAQIFYLHFTATLVP
                     TWIPPNQTFWAILTTVAFALAAVAMLVNRRARLAAGLMTLMLALFGVLVWVPRVIGHP
                     DAHGNWSEFALNFLITGAAWMVAVMRSSDSIR"
     gene            3601..5313
                     /locus_tag="VFU90_14310"
     CDS             3601..5313
                     /locus_tag="VFU90_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481015.1"
                     /translation="MHVPHDYRAVVSGLCGATWLLGLWYAAPLIQPLRTRPQRAAATM
                     ALGCMLPLVLGFVHLLYGWSLWIVLGLIVAARAALTRRGRIEAVTPEGRLWDLLVGFG
                     VLVALAWPVAVRPTLDGDTLIYHLPNAASWVAQHGIWTTGTRYWWYPPASELFGAGLL
                     ATGGINVIGWAGILPGALLLLTVREAGRRLGAPPLAGTLMACALLATPVAAVQLVSLQ
                     NDLWLASLFLFTLTEYALPAFGTLALTKPYGILYALVASLTCRERWRVISALAVSIAA
                     IGVWVLRDVVLLPHAIVPLGTSAGETNLGTTIAAHFPHSLVVLATASWHAGVVWTLLF
                     AAGLLAIPFSRDGRLRWGALAATIFFSIIPTAYSGGPVQQLATGASLRFALPVAAAGL
                     VWMLAFERRVRAVLAAAAGIGSLIGIGVQWQLFRNDATTNDTPAIVAAVVLAVAASFL
                     LTNDRLRIAVYAALFLVLGGLAGGLAGSHPADYIADRYGGAFAFVSSERIDRVVTLGL
                     PAGAAITVDPRIAAYDGLDEGTCGEAKAVNAVIVASTDRLRSLACVRILYRDARTAVI
                     DPRD"
     gene            5428..5772
                     /locus_tag="VFU90_14315"
     CDS             5428..5772
                     /locus_tag="VFU90_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481016.1"
                     /translation="MQFYLKRAMYVTLAACLFGSATSAVAAVASVQSAPTNVALAGPR
                     DHDGDRHDPRQGRDKGDQRDNGDRGYLHTKMDKGDPGDKSKDGNRDKRGKGDQGEGDQ
                     QDKGDPGQSPLA"
     gene            5781..6599
                     /locus_tag="VFU90_14320"
     CDS             5781..6599
                     /locus_tag="VFU90_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481017.1"
                     /translation="MAAREFRSLGRLLVAAACAAAVFPLPAVADGTPAHTFASVSATM
                     STTVGSDVYAATSQESEAWSNLEVTAGWRNLYPSGGTYFTVQAARHIGLFPERASLYG
                     GALGALVPGDDSHERFQIVQEFDDPKTGVERFEASAGMISWHGSTPYHERYVALDYFA
                     GAVSRFGFDGPVRRFDVYTSYAQTLDGHVVEVSPTYSLPLGDRGRTSGWLSLTGDVPI
                     GATVERYASVGAGVTRQVSRRLELAASATRYWSRDEVGAYDRRLVFSAGARLRW"
     gene            6755..8164
                     /locus_tag="VFU90_14325"
     CDS             6755..8164
                     /locus_tag="VFU90_14325"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5481018.1"
                     /translation="MLPLIFILPIVLVLLLRIAEVRHTESIEERSDTVISETQTVRAE
                     VIGMQSALRAYLLTGNPAFKQDFASRERLVDATLAAIGEDAAGDPAMTARVSRLAPHT
                     RAWMAYASAVMARSRTSQREGAGIGGDETYMAPVTRDVGAILAAKSSERGRVISRAEH
                     GIRTLLLIVVVCALLATLATALSVRRMLILAEIERQAEQERARLREARRRTAELEREN
                     HRFKTADKFKSEYLATMSHELRTPLTAMLGFSEVIRDGKVGPITPEQADCLDHIVKSG
                     HHLLELVGSVLDLAKVEAGKLDVAMIVSDPRLPVRDVVDGMRELAARKSLRLELDLAT
                     SPREALVDPSRLRQILYNYLSNAIKFTPAGGTVRVRLLPEGSDGFRLEVADSGIGIAD
                     ADVKTLFKEFGQIASHETAAQKGTGLGLALTKRLTEAQGGYVGVKSTLGAGSTFFAVF
                     PQAVWAAGSQRKPRETALA"
     gene            8157..8528
                     /locus_tag="VFU90_14330"
     CDS             8157..8528
                     /locus_tag="VFU90_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5481019.1"
                     /translation="MRKQILVIDDNVANLRLFEILLRSDYVVETAADAPEALAVLRRF
                     HPDLILMDVQLPGMDGISLTRHLRTTKEYKAIPIVALTAYAMLDDRKRALEAGCDGFI
                     SKPVDTRTFIDTVERHLEKAS"
     gene            8525..9226
                     /locus_tag="VFU90_14335"
     CDS             8525..9226
                     /locus_tag="VFU90_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5481020.1"
                     /translation="MTEPTTVLIVDDDPLIAKMLRPYLDEERFRTLVAANTEEAARTL
                     ERESPAILLLDVTMPGENGLDLLRRLHGRGDVGVIVLTGRTDELDRILGLELGADDYV
                     TKPFSPRELVSRIKAVLRRSRNSASPERDRRRIVAGDLSIDTRERSVTRGNTEISLTP
                     TEFRILEVLASERGRAFSRAELLDRVNADALDVAERTLDSHITRLRQKLEPDSAHPRY
                     VLTVYGHGYKFSKTA"
BASE COUNT         1580 a         2957 c         3043 g         1663 t
ORIGIN      
        1 gatctcgagg tcgtcgagtt cgatttgctc tcgcaaatcg aaggcatcgg cggaatgctc
       61 gttccgcagg cgcacgccaa acaaatcggg ttgatgacgt acgtcgatcc cgatatcccg
      121 ttccggctgc tcggcgatcc cgtccggttg cggcaagtgc tggtgaatct cgccggaaac
      181 gcgatcaaat tcacgtcgaa aggctgcgta gcggtcagcg ccgatctgat gtcggcggga
      241 gcgcagcacg tgtcgatccg gttctcggtg caggatacgg gtatcggtat cgagcccggc
      301 attttgccgt cgctcttcga ggcgtttacc caggccgacg gctcgacgac tcgccgctac
      361 ggcgggaccg ggctgggcct ggcgatctcc aagcagctcg tcgagctgat gggtggaagc
      421 atcgaagtcg agagccaacc cggggccgga tcgacgttct cgtttacgct cgagatgcgc
      481 gtcggctcca acgtacgccg gctcccggcc cgggcggaag tgcgcggtac gcgcgttcta
      541 atcgtcgacg acgacaagat gtcgcgcgat attctgtcgc gttacgtcac ggcgtggggg
      601 atgattgcga aaaccgcgca aggggcgggc gaggcgctcg aaatgctcac gaggagcgcc
      661 gaagggccgg agcgtttcca catcgcgatc gtcgacttac gcatggccgg ggtggacggc
      721 atccggctcg gctacgccat ccggcgaaac cgcgcgatcg cgcaaaccaa gctcgtgctg
      781 gtgacggcct acgatgcgcc gaccgtcggg caagcggcga ttcgcgccgg cttctcgtgc
      841 tatctcacca aaccgattcg gcattcacag ctgtacgacg cgatctcgga cacgctgctc
      901 ggctcggtac cggttacgat caagccagcg gtgcgggcgg tcgaagcggc acatcgcgat
      961 acgattctgt tggccgaaga taacgccgtc aaccgcaccg tctcgctcca gcagttgaag
     1021 aaactcggat atacggtcga cgccgtcgcc aacggcaagg aggccgtcga acgcgtggcc
     1081 ggcggtacgt acgggctggt tttcatggat tgccggatgc cggtgatgga cggattcgag
     1141 gcgacgcggg ccatccgaaa aatcgaaagc cgtaccggga gacgcgtgcc gatcgtagcg
     1201 atgacggcca acgcactctc gaacgatcgc gacgactgtc tcgcggccgg gatggacgac
     1261 tacctgacca agcccgttgg gctcgacgac ctgcgggcgg tgctgaaacg ctggctcggc
     1321 gagtcggagg aaagcacggt catcgacagc gatcgcatca gcggcatttt cggcggggac
     1381 cgggatgcgg tcgtcgagtt cttcgcgagc gtcgtaccga gcatcggcgc gctctgcgag
     1441 aagatcttcg agaccagcga cgtcgcggag ctgcgcgagc tcgtacacga actcaaaggc
     1501 gccgcgtcca acatcggggc gcgcgagctg gcgattgcgg ccgccgatct cgagcgcggc
     1561 ctggcgggcg gttccgataa ccaggcggcg ctgcaaatct tgctgaccaa actggaacag
     1621 tcgtgggaac gtttccgcga agcgatggac aatcagcccg aagcgagttt cggttagtat
     1681 gatcgttctg gccgtcgacg attccgacat caacctcgcc gtctaccgcg ggatgctcaa
     1741 gcagattccc gacgtgcagg tggtatgcca tacgtcgccg ctcgacgcgc tggcgtgggc
     1801 tgccggcaac agcgccgacg tcgtggcggt cgactatcac atgcccgaac tcgacgggtt
     1861 gcgtttcgtc gagcggtttc gcacgctcgc gcgcagcgcg cacgcgctga tcgtgatcgt
     1921 aacggcggat acggaaatcg gcgtacggca ccgggcgctc gaaatggggg cgaacgactt
     1981 cctcaccaag ccgatcgacc ggatagactt taccgcgcga attcgcaatc tcttggaaat
     2041 tgcgagaagc cggcggcagc tcgcggatcg tgccgaatgg ctgcgcgagg aagtggagcg
     2101 cgcgaccgcg tcgctggccg cccgcgagat cgaaacgatc gtccgcctga cgcgcgcggc
     2161 ggaaacccgc gacgacgtca ccggcatgca cgttattcga gtcgggcaca tttgcgcggc
     2221 gttggcgcga accgtcggct tgccggacga cgagtgcaaa acgttgctgc tggcggcgcc
     2281 gatgcacgac atcggcaagg tggcaacgcc ggacagcatc cttttcaaac cgggcccgct
     2341 gaccgagcga gaaggcgccg tcatgcgaaa tcacacgatc gccggttacg agattctaaa
     2401 agacagcgaa tcggagatgc tgcagtgcgc ggccgagatt gcgctgacgc accacgaatg
     2461 cttcgacgga agcggctatc cgcgcgggct caaggggacg gccatcccgc tcgccggccg
     2521 gttgtgcagc gtcgccgacg tcttcgacgc gctgacctcg atccggccgt acaaactcgc
     2581 ctggagcgtg gagaaagccg tcgaaatgct cgatgaggga tgcggcacgc aattcgatcc
     2641 cgagatcgtc gcggcattcc accgggcgct cgacgatatc ctcggcatca aacaccggtt
     2701 cgccgaccaa ggcgccgcga ccgacaaact cgcgcgcgcc tacggccact ttcagtagcc
     2761 caggtgaacc cgcggtcaca ggcctaggcg tgcaagcgtt gcctttccta tgaaaacggc
     2821 gccgtacggg cggatcgtgt tcggtgcgtc ggcggtgttg tccggcgtca tcgcgctgat
     2881 gtggcacgac gccgacacgt ggcaaagcct ggcgaagttt tggagcctgc cgttcggcct
     2941 catcgtcggc ggatgcctca tggcggcgca aattgccggc ggcgtcgccc tagcataccc
     3001 ccgaagcgcg cgcgcggcgt cgatcgtcct cggcatcgtc tacgtgctgt tctcgctgat
     3061 ctgcattccc ggtatcgtcg ccgcgccggc ggtctacgta cagtacggag cgttcttcga
     3121 gcagttctcc ctcgtgtgcg gcgcgctcgc cgtgtatgcg gcaaccgaac cggacgccgc
     3181 ccgcgcggcg gccctcggcc gagcggcccg tatcgggatg gggctctgcg cggtttcgtt
     3241 cacgctggcc cagatcttct atctccattt taccgccacg ctggttccaa cgtggattcc
     3301 gccgaatcaa acgttttggg cgatactcac gacggtcgcg ttcgcactcg cggcggtcgc
     3361 gatgctcgtt aaccgtcgcg cccgtctcgc ggccggcctc atgacgctga tgctcgcgct
     3421 cttcggcgtg ctggtttggg taccgcgcgt gatcggccat ccggacgcgc atgggaattg
     3481 gtccgaattc gcgctgaact tcctgattac gggagccgcc tggatggtgg ccgttatgag
     3541 gtcttccgac tcgatcaggt agaccggcgt caccgtcgaa tccggatacg ctgcaacgtc
     3601 atgcacgtac ctcacgacta tcgcgccgtc gtttcggggc tttgcggcgc gacctggctg
     3661 ctcgggctct ggtatgcggc cccgctcatt caaccgctgc gaacccggcc gcagcgtgcg
     3721 gcggcgacga tggcgctggg atgcatgctc ccgctcgtcc tcggcttcgt ccacctgctc
     3781 tacggatggt ctctctggat agtcctgggt ctgatcgttg ccgcgcgcgc cgcgctaacg
     3841 cgtcgcggtc gcatcgaggc ggtaactccg gaaggccggc tttgggattt gctcgtcgga
     3901 ttcggcgtgc tcgtagcgct tgcatggccg gtcgcggtcc gtcccaccct cgacggcgac
     3961 acgctcatct accacctgcc caacgcggca agctgggttg cgcaacacgg gatctggacg
     4021 acggggacgc ggtattggtg gtatccgccg gcttcggaac ttttcggcgc cggtttgctg
     4081 gctacgggcg gaatcaacgt gattgggtgg gccgggatac tcccgggtgc gctgctgctc
     4141 ctcacggttc gagaggcagg ccggcggctc ggtgcgccgc cgcttgccgg gacgctgatg
     4201 gcatgtgcgc tgctcgcaac gccggtcgca gccgtgcagc tggtgtcgct ccagaacgac
     4261 ctttggctcg catcgctgtt tctgtttacg ctgaccgagt acgcgctgcc ggcattcggc
     4321 acgctcgcgc tgacgaagcc gtacggcatt ttgtacgcac tggtcgcaag cctaacgtgc
     4381 cgcgagcggt ggcgagttat cagcgcgctg gcggtttcga tcgcggcgat cggcgtgtgg
     4441 gtcttacgcg acgtcgtact gctcccgcac gcgatcgttc cgctcggaac gtcggccggc
     4501 gaaacgaacc tcggcactac gattgcggcg cacttccccc attcgctcgt ggtgctcgcg
     4561 acggcatcct ggcacgcggg cgtggtctgg acgctgctct tcgccgcggg tctgctggcg
     4621 ataccgtttt cgcgggacgg ccggcttcgt tggggtgcgc tcgccgcgac gattttcttt
     4681 agcatcattc cgacggcgta cagcggcggc ccggtccagc aactggccac cggcgcatcc
     4741 ttgcgctttg cgttgcccgt ggcggccgcg ggactcgtgt ggatgctcgc gttcgaacgt
     4801 cgcgttcgtg ccgtgttggc cgccgcggcg ggcatcggca gcctgatcgg gattggcgta
     4861 cagtggcagc tctttcgcaa cgacgccacc acgaacgata cgccggcgat cgtcgctgcc
     4921 gtggtgctcg ccgtcgcggc ctcgttcctg ttaaccaacg atcggctgcg gatcgccgtt
     4981 tatgcggccc ttttcctcgt cctgggtggg ctcgccggcg gcctggccgg cagccatccc
     5041 gccgattaca tcgccgatcg ctacggcggc gcgttcgcgt tcgtttcgtc ggaacggatc
     5101 gatcgggtgg ttacgctcgg tttgccggcc ggagccgcga tcaccgtcga cccgaggatc
     5161 gcggcttacg acgggctcga cgaaggaacg tgcggggaag ccaaggcggt gaacgccgtc
     5221 atcgttgcga gcaccgacag attgcggagc ctcgcttgcg tccgaattct ataccgcgac
     5281 gcgagaaccg ccgtcatcga tccgcgagac taagcgaacc tcaaccaatt ttcaacagga
     5341 accccatcgc gcttcaatcg cgaccggtta ccgtacagaa agcgggtcaa cccgcaacgg
     5401 ttcaacggaa gggaaggggt tttcatcatg cagttctatc tcaagcgcgc gatgtacgta
     5461 acgctggcgg cctgtctgtt cggcagtgct acctcggccg tcgccgccgt cgcttcggtt
     5521 caatccgcgc cgactaacgt ggcgttagcc ggcccgcgcg atcacgacgg cgaccggcac
     5581 gatccgcgcc agggccgcga caagggcgac cagcgcgaca acggcgaccg tggttacctc
     5641 cacaccaaaa tggataaggg cgatcccggc gacaagagca aagacggtaa ccgcgataag
     5701 cgcggcaagg gcgaccaggg cgaaggcgat cagcaagaca agggcgatcc cggtcagtcc
     5761 ccgctggcgt agcaggaacg atggcggctc gggagttccg ctctttgggc cgcctgctcg
     5821 tcgcggcggc gtgcgcggca gcggtgtttc cgctgccggc cgttgccgac ggcacccccg
     5881 cccacacgtt tgcgagcgtt tccgcgacga tgtccacgac cgtcggatcg gacgtatacg
     5941 cagcaacgtc gcaggaaagc gaggcgtggt cgaaccttga ggtgacggcg ggctggcgca
     6001 acctgtatcc cagcggcgga acttacttca ccgtgcaggc cgcgcggcac atcggcctct
     6061 tccccgaacg ggccagtctg tacggcggcg cgttaggggc gctcgtaccc ggcgacgatt
     6121 cccacgaacg gttccaaatc gtccaagaat tcgacgaccc gaagacggga gtggagcgtt
     6181 tcgaggcgtc ggccggcatg atttcgtggc acggaagcac gccgtatcac gagcgctacg
     6241 ttgcgctgga ctactttgcg ggcgccgttt cccgattcgg cttcgacggg ccggtgcgac
     6301 ggttcgacgt ttacacgagc tacgcgcaga cgctcgacgg tcacgtcgtc gaggtttcgc
     6361 cgacctactc tctaccgctg ggcgaccgcg gccgcacgtc gggctggctg tcgctgacgg
     6421 gcgacgtacc gattggggca accgtcgagc gctacgcaag cgtcggcgcc ggggtgacgc
     6481 gtcaggttag ccggcggctg gaactggccg cctcggccac ccgctattgg tcccgggatg
     6541 aggtcggcgc gtacgatcgg cgcctggttt tcagcgcggg tgcgcgcctc agatggtgac
     6601 ttttatcggc gtccgccggt tcttcttcag aatttcccaa aattcgacgc acgcggacga
     6661 tatgatgagc tcgcagtttc ccttggagta tgcacgacat tcgatgagcg ccgaagctag
     6721 gggtcgcagg ctgcgacaga tcgccgggat tgccgtgctg cccctcattt tcatcctgcc
     6781 gatcgtgctg gttctactgc tgcgcatcgc cgaggtccgg cacaccgagt cgatcgagga
     6841 gcgctcggat acggtcatta gcgagacgca aacggtacgg gccgaggtca tcggcatgca
     6901 gtcggcgctg cgcgcgtact tgctcacggg gaacccggcc ttcaagcaag acttcgcgag
     6961 ccgggagcga ctcgtcgatg cgaccctggc cgcgatcggt gaggatgcgg ccggggatcc
     7021 cgccatgacg gctcgggtga gccggctggc gccgcatacg cgcgcgtgga tggcctacgc
     7081 ctccgcggtc atggcccggt cgcgcacgtc gcagcgcgag ggtgccggga tcggaggcga
     7141 cgagacgtac atggcaccgg tcacgcgcga cgtcggtgcg atcctggccg cgaagtccag
     7201 cgagcgtggc cgcgtgattt cgcgggccga gcacggcatc cggacgttgc tgctgatcgt
     7261 cgtcgtgtgc gcgctgctgg ccacactcgc gaccgcgctt tcggtgcgcc ggatgctgat
     7321 tctcgcggag atcgagcggc aggcagaaca agagcgcgcg cgcctgcgcg aagcgcgccg
     7381 tcgcaccgcc gaactagaac gggaaaatca ccgcttcaag acggccgaca agttcaagag
     7441 cgaatacctc gcgacgatgt cgcacgagct gcgcaccccg ctcaccgcca tgctcggctt
     7501 cagcgaagtc attcgcgacg gcaaggtcgg gccgattacg ccggaacagg ccgattgcct
     7561 cgatcacatc gtcaagagcg ggcatcacct cctggaactg gtcggcagcg tactcgacct
     7621 tgcgaaggtc gaggccggaa agctggacgt ggccatgatc gtctccgatc ctcggctacc
     7681 ggtgcgcgac gtggtcgacg gcatgcgcga gctggccgca cgaaaatccc tgcgcctcga
     7741 gctcgatctc gcgacctcgc cgcgcgaggc gctcgtcgat ccgtctcgcc tgcgccagat
     7801 tctctacaac tatctctcga atgcgatcaa gtttacgccg gcgggcggta cggtccgagt
     7861 gcgccttttg cccgaaggca gcgacggttt ccggctcgag gtcgccgaca gtggaatcgg
     7921 tatcgccgac gccgacgtca agacgctctt taaggaattc ggacaaatcg cttcgcacga
     7981 gacggctgcc cagaagggta ccggtctcgg tctcgcgctc acgaagcggc tcaccgaagc
     8041 gcaaggcggc tacgtcgggg tgaagagcac cctcggtgcc ggcagcacgt tctttgccgt
     8101 gtttccacaa gccgtttggg cggctgggtc gcagcgcaag cctagggaga cggctcttgc
     8161 gtaagcagat actcgtgatc gacgataacg tcgcgaactt acggctcttc gagattctgc
     8221 tgcgtagcga ctacgtcgtc gagacggctg cggacgcgcc ggaagccctg gcagtcctcc
     8281 ggcggtttca tccggacctc atcctgatgg acgtgcagtt gccgggaatg gacgggattt
     8341 cgctcacgcg ccatctgcgc actacgaagg aatacaaagc catcccaatc gtcgctctga
     8401 cggcgtacgc gatgctcgac gatcgcaagc gcgctctcga ggccggctgc gacggcttca
     8461 tttcaaagcc cgtcgatacc cgaacgttca tcgatacggt cgagcggcat ttggagaagg
     8521 cttcgtgacg gaaccgacga ccgtgctgat cgtcgacgac gacccgctga tcgcaaaaat
     8581 gctgcgcccc tacctcgacg aagagcgttt tcgtacgctc gttgccgcaa acaccgaaga
     8641 agcggcgcgc accctcgagc gcgaatcgcc ggcgatcctg ttgctcgacg tcacgatgcc
     8701 cggcgaaaac ggcctcgacc tgttgcggcg tctgcacggg cgcggcgacg tcggggtcat
     8761 cgtcctgacc gggcggaccg acgagttaga ccggatcctc gggctcgaac tcggtgcgga
     8821 cgactacgtg accaagccct tcagccctcg cgagctcgtt tcgcggatca aagcggtgct
     8881 gcgccggtca cggaattccg ctagccccga gcgggaccgc cgccgaatcg tcgcgggcga
     8941 cctttccatc gacacgcgcg aacgttcggt cacccgagga aatacggaga tatccctgac
     9001 gccgacggaa tttcggattc tcgaagttct ggcgtcggag cgcggacggg cgttctcccg
     9061 ggcggaactg ctcgaccgcg tcaacgccga tgcgcttgac gtcgccgaac gaaccctcga
     9121 cagccacatc acgcggctgc ggcagaaact cgaacccgac agcgcgcatc ctcgttacgt
     9181 gctgacggtt tacgggcacg gctacaagtt cagcaagacc gcgtaaaccg cacgtacggc
     9241 acc
//
LOCUS       DASUCJ010001226         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_28374, whole genome shotgun sequence.
ACCESSION   DASUCJ010001226 DASUCJ010000000
VERSION     DASUCJ010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28374"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFU90_14340"
     CDS             <1..246
                     /locus_tag="VFU90_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481021.1"
                     /translation="HLPFADEAFELVVLMNAIPFFDELARVTAPGGTVAHSFSRGDET
                     PIYVPDERLRAELGRRGFSEFATLSAPPATAFRARKL"
     gene            404..847
                     /locus_tag="VFU90_14345"
     CDS             404..847
                     /locus_tag="VFU90_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5481022.1"
                     /translation="MKITLLALALVASAVFAVPSGAAARQPIVAKALAVGTMNATVVH
                     ATTGAMVLDRITIAPGGNFGWHTHGSPVAVVVTGGTLTVFDPSVGKCAPFKVSKGQSF
                     VEPANHIHLARNDTAKPVTLYAMYLGIPKNAGANVPAKQPAGCGA"
     gene            complement(851..>1221)
                     /locus_tag="VFU90_14350"
     CDS             complement(851..>1221)
                     /locus_tag="VFU90_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5481023.1"
                     /translation="FATEMAGPRGEDVRRAWVEDGAGPTAGDEVRRHIVDTMCSVPLP
                     IARAMIRGLNEWNGVGALALCEVPLLILNPTGRSNEPSRIRPLNPGMRIGATVGAGHF
                     HQLETPDQVNAMIGRFLELC"
BASE COUNT          188 a          418 c          418 g          197 t
ORIGIN      
        1 catcttccgt tcgccgacga agccttcgag ctcgtcgtcc tcatgaacgc gattccgttc
       61 ttcgacgagc tcgcccgggt gaccgcgccg ggaggcaccg ttgcgcactc gttctcgcgt
      121 ggcgacgaga cgccgatcta cgtgccggac gagcgcctgc gtgcggagct cggccgccgg
      181 gggttctccg agttcgcgac tctttccgct cctccggcga cggcatttcg ggcgcggaag
      241 ttgtagtgtg aggagcaacg gacgttcgcg acacggttgc accggtccgg acgcctgcag
      301 cccccgagca atcgggggct ctttcttgct aaggctgcgc aaagggcgcg caaagtgccg
      361 ccttctagcg tgcccggacg tcaggtacgc caaaggagga gagatgaaga tcacactgct
      421 cgcactggcg ctcgtcgcaa gcgccgtctt cgccgttccg tcgggcgccg cggcccgcca
      481 gcccatcgtc gcgaaggcgc ttgccgtcgg cacgatgaat gcaaccgtcg tgcatgcgac
      541 gacgggggca atggtgctcg accggatcac gatcgcgccc ggcggcaact tcggatggca
      601 cacgcacggg tcgccggtcg cggtcgtggt gacgggcggc acgctcaccg tcttcgatcc
      661 gtcggtcggc aagtgcgcgc cgttcaaggt gtcgaagggg cagtcgttcg tcgagcccgc
      721 gaaccacatc catctcgcgc gcaacgacac tgcgaagccg gtgacgctgt acgcgatgta
      781 cctcggtatc ccgaagaacg caggggccaa cgtgccggcg aagcagccgg ccggctgcgg
      841 cgcgtagcgg ctagcagagc tcgaggaagc gcccgatcat ggcgttgacc tggtcgggcg
      901 tttccagctg gtggaagtga ccggcgccga cggtcgcgcc gatgcgcatg cccgggttga
      961 gcgggcggat ccgcgaaggc tcgttgcttc gccccgttgg attgaggatc agcaacggaa
     1021 cctcgcacaa cgcgagcgcg ccgacgccgt tccactcgtt gaggccgcgg atcatcgccc
     1081 gcgcgatcgg cagcggcacc gagcacatgg tgtcgacgat gtggcggcgc acctcgtcgc
     1141 ccgcggtcgg accggcaccg tcctccaccc acgcgcgccg cacgtcctcg cccctcgggc
     1201 ctgccatctc ggtcgcgaac g
//
LOCUS       DASUCJ010001227         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1465072, whole genome shotgun sequence.
ACCESSION   DASUCJ010001227 DASUCJ010000000
VERSION     DASUCJ010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465072"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFU90_14355"
     CDS             complement(<1..1197)
                     /locus_tag="VFU90_14355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5481024.1"
                     /translation="MNVTFHGAAREVTGSCHVVHVGPSSVALDFGLFQGHRRDADDKN
                     RALPFLPAHLNAVILSHAHLDHAGRLPLLARLGFGRPIFATPATRDLCAIMLADAARL
                     AERDADYLRRRERPVVAPLYDEKDVASLMPRFVCVPYDTPFAVAPGVRATFVDAGHIL
                     GSASVVLECSENGATRRLVYSGDIGRRGLPIIRDPRPPNGADAVIMESTYGDRDHSPV
                     ADMPGQLARVVRDTAVRGGRVFIPAFAVGRAQELLHDLEMLRHANAIPAIPIILDSPM
                     AIAATATYAAHREDLDLGERLVRTERDFLSFEGLELTQDVDASKALNSRNGRMVIIAG
                     SGMVEGGRILHHLLRGAPDPRNTILIVGYMADNTLGRRIVERRTVLRILGEDVPLRAR
                     VEVLNGY"
     gene            complement(1194..2288)
                     /locus_tag="VFU90_14360"
     CDS             complement(1194..2288)
                     /locus_tag="VFU90_14360"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481025.1"
                     /translation="MLSLQELASISRELRDQHVLSVYVDAAFDDPALRTAWRPQADHA
                     IKAIRRRLEAAPHAERTNFERCVTFLDSELSAFAGGIGAPGWFAFITADGVRAAEKIP
                     APMPTLALWMIGPCIAPYIRVLKQERPVVVIVAGASQARLYRYITGVLEPAPSIHAHA
                     TVEEPAHMGDTPRLGFHAGTRGSTGRDAAQLAHHVGTERMIHEATRESLKIAGRHGWI
                     VVGGIPEVGAHITRALEPLAPDRVIHLEGVEVHATDAGVREAAERAASRLRDQRDLRQ
                     IAEITGAAVDDRWVVRGAVATRRALDSGSVRELYFTPRFLESSLVDAEYSVRAALAQS
                     AEVEEVSRTVAERLDEFGGVGARLRFAAPL"
BASE COUNT          459 a          834 c          773 g          399 t
ORIGIN      
        1 gtagccgttg agtacttcga cccgcgcccg gagcgggaca tcctcgccga gtatccgcag
       61 cacggtccgc cgctcgacaa tgcggcgtcc cagcgtgttg tcggccatgt agcccacgat
      121 gagaatcgtg ttgcgaggat ccggagcgcc gcgcagaaga tgatggagta tgcgcccacc
      181 ctccaccatt cctgaaccgg cgatgatgac catccgacca tttcgactgt tcagcgcttt
      241 cgatgcatcg acgtcttgcg tcagctccag gccttcgaac gacaggaaat cgcgctccgt
      301 ccgtaccagc cgctcgccga ggtcgagatc ctcacgatgc gcggcgtatg tcgccgtcgc
      361 cgcgatggcc atcggactgt cgaggatgat cggaatggcg ggaatcgcgt tcgcatgccg
      421 cagcatctcg aggtcgtgca ggagttcttg cgcgcgtccc acggcgaacg ccgggatgaa
      481 cacgcgaccg ccgcgcaccg ccgtgtcgcg cacgacccgc gccagctgcc ccggcatgtc
      541 cgccaccggc gagtggtcgc ggtcgccgta ggtcgattcc atgatcaccg cgtcggctcc
      601 gttaggcgga cgcggatcgc ggatgatcgg caggccgcgc cgcccgatgt cgcccgagta
      661 caccaggcgt cgcgtcgccc cgttctccga acactcgagc acgaccgacg ccgacccgag
      721 aatgtgcccg gcgtcgacga acgtggcacg aacacccggc gccaccgcga acggcgtgtc
      781 gtacggtacg cagacgaagc gaggcatgag agacgcaacg tctttctcat cgtacagcgg
      841 cgccacgacc ggccgctccc gccgcctcaa gtagtcggcg tcacgttcgg ccaggcgcgc
      901 cgcatcggcc agcatgatcg cgcacagatc tcgcgtggcg ggtgttgcga agatgggccg
      961 accgaatccg aggcgcgcca gtagcggcag gcgtccagcg tgatccaggt gcgcgtgtga
     1021 taggatcacg gcgttgaggt gcgccggcag gaatggcagc gcgcggttct tgtcgtctgc
     1081 atcacggcga tgaccctgga acaacccgaa atccagcgcg acggacgacg gcccaacgtg
     1141 cacgacgtga cacgaacccg tcacctcacg cgccgcaccg tgaaacgtga cgttcacagc
     1201 ggagcggcga agcgcaaccg agcgccaaca cctccgaact catccagtcg ttcggccaca
     1261 gtgcgcgaca cttcctccac ctcggcgctc tgcgcaagcg cggctcgtac cgaatactcc
     1321 gcatcgacca gactgctttc cagaaaccga ggggtgaaat acaactcgcg cacagatccc
     1381 gagtcgagcg cgcgtcgcgt cgcaacagca ccgcgcacca cccatcggtc gtcgacggcc
     1441 gcaccagtaa tctccgctat ctggcgcaaa tcgcgttggt cgcgcaatcg ggatgcggcg
     1501 cgctccgccg cctcccggac gccggcgtcg gtcgcatgga cctcgactcc ctccaggtga
     1561 attacccggt ctggcgcgag cggctcgagc gcgcgtgtga tatgagcccc gacttccgga
     1621 atgcccccga cgacaatcca tccgtgccga ccggctatct tcaggctttc ccgcgtcgct
     1681 tcgtggatca tgcgctccgt gccgacatga tgcgccaact gggccgcgtc acggccggtc
     1741 gaaccgcgtg tccccgcgtg aaagccgagg cgcggcgtat cacccatgtg agccggctct
     1801 tcaacggtcg cgtgtgcgtg aatgctaggc gctggttcta acactccagt gatgtaccga
     1861 taaagacggg cttgggaggc gccggccacg atgacgacga cgggtcgttc ctgcttgagc
     1921 acacggatgt acggcgcgat acagggcccg atcatccaga gagccaaggt cggcatcggc
     1981 gcaggaatct tttcggccgc gcgcacgccg tcggcagtga tgaacgcaaa ccaacccggc
     2041 gcgccaattc cgcccgcgaa tgcagaaagc tcactgtcga ggaacgtgac gcagcgctcg
     2101 aagttcgtcc gctccgcatg gggcgccgct tccaagcgtc gacgaatcgc cttgattgcg
     2161 tggtcggcct gcggccgcca tgcggttcgg agcgctggat cgtcgaaagc tgcgtcgaca
     2221 tacacgctga gcacgtgttg atcacgcaac tctcgagaga tcgaagcgag ttcttgaagt
     2281 gacagcattg ccacatctcc actgtgagca ccaacagatt gtcacgcgtc gcgcacgtgg
     2341 acaaagctcg gcggagaaca gcgcacggtc cgtcggggaa tcgtgtaggc ggcgtcgggt
     2401 tagagcgcac ggctcagatc tcgtgaaccg cgtccgaagc gacccggact catccgggtt
     2461 tactc
//
LOCUS       DASUCJ010001228         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_777125, whole genome shotgun sequence.
ACCESSION   DASUCJ010001228 DASUCJ010000000
VERSION     DASUCJ010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777125"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1608)
                     /locus_tag="VFU90_14365"
     CDS             complement(<1..>1608)
                     /locus_tag="VFU90_14365"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481026.1"
                     /translation="LGSYGFRDALDYSRPEPGRRYAVVRTFMAHHIGMGLVALTNALT
                     ADVWQRRFHADPMVRSASLLLHERIPRRLELQDTQPSRSDEARSASDVERAAVREFDT
                     PDTVQPHVALLGRAPYTIMVSHAGGGYSRFEDLAVTRWRADGTRDHSGQFCYLKDIAR
                     GAVWSAAHQPVCVEADRYKAVLANDRVTFHRADGDIETRTEIGVVPDDAAEVRRVVVT
                     NNSDASRDVELTSYGEIVLAPPDADRAHPAFGNLFIETEWHAWCSAITAIRRPRSANE
                     RPLWCVHVVDTGAERVGLTSCETDRARFLGRGRSVRDPVALDVDGPLSGTTGAVLDPI
                     FALRTRVRLAPGQSASVAFTTLVATTRERAFELAGRYHDPHAAQRALDLAWTSSQVEL
                     NELKITPADAATFQELAGHLFYSCPELRAPQSELRRNNGSRARLWANGISGDWPIVLA
                     TIDSADGLPTLRQLFAAHHFWRRHGMTVDLVVLNEQPHTYLQALNEQITATLFASSDS
                     SVTDRPGGAFVRRRDLLSAADLLMLRAT"
BASE COUNT          281 a          526 c          548 g          253 t
ORIGIN      
        1 ggtcgcgcgc agcatgagca ggtcggcggc gctgagcagg tcccgccgcc taacgaaggc
       61 accgccaggc cggtcggtga cgctcgaatc actcgatgcg aacaacgttg cggtgatctg
      121 ctcgttgagc gcctgcagat acgtatgcgg ctgctcgttc agcacgacga gatcgaccgt
      181 cataccgtgg cgccgccaga agtggtgtgc cgcgaacaat tggcgcagcg tcggcagtcc
      241 gtcggccgag tcgatcgtcg ccaggacgat cggccagtcg ccggaaattc cgttagccca
      301 gagtcgagct cgcgagccgt tattgcggcg gagctcggat tgcggcgcgc gcagttccgg
      361 gcacgaatag aagagatggc cggccagctc ctggaaagtg gctgcatccg ccggagtgat
      421 cttgagctcg ttcaattcga cttgcgagga cgtccacgcc aggtcgagcg cgcgctgcgc
      481 cgcgtgtgga tcgtggtagc gaccggcgag ctcgaaggcg cgctcgcggg tcgtcgcgac
      541 gagcgtcgtg aacgcgaccg acgccgattg gcccggcgcc aatcgaacac gcgtgcgcag
      601 cgcaaaaatg ggatcgagga cggcgcccgt ggtaccggac agcggtccat cgacgtcgag
      661 cgcgaccggg tcgcgaaccg aacggccgcg gccgaggaac cgcgcacgat cggtctcgca
      721 gctcgttagg ccgacgcgct ccgcgccggt gtcgaccacg tggacacacc acagcggtcg
      781 ctcgttggcc gaccggggac gacgaatggc cgtgatcgcc gagcaccagg catgccactc
      841 ggtctcgatg aagagattcc cgaatgccgg atgcgcacga tccgcatcgg gcggtgcgag
      901 aacgatctcg ccgtaactcg tcagctcgac gtcccgcgag gcatccgagt tgttggtcac
      961 gaccacccgc ctcacttcgg ccgcgtcgtc agggacaacg ccgatttccg tgcgcgtttc
     1021 gatgtcgccg tcggcgcggt ggaacgtgac gcgatcgttg gccaggacgg ccttgtaccg
     1081 gtcggcctcg acgcacaccg gctgatgcgc ggcagaccac actgccccgc gcgcgatatc
     1141 cttgaggtag cagaactgcc cgctgtgatc tcgcgttcca tccgcccgcc accgcgtgac
     1201 cgcgaggtcc tcgaatcggc tgtagccgcc gccggcatgg ctcaccatga tcgtgtaggg
     1261 cgcacgtcct aacagggcga cgtggggctg cacggtgtcc ggtgtatcga actcacgaac
     1321 cgccgcgcgc tccacgtcgg aggcagaacg tgcttcatcg gaacgcgacg gctgcgtgtc
     1381 ctgcagctcg agccgtcgcg gaatgcgctc gtgtagcagc aagctcgccg agcgcaccat
     1441 cgggtccgcg tgaaaacggc gttgccacac gtcggcggtg agcgcattcg tgagtgcgac
     1501 gagtcccatg ccgatgtgat gcgccatgaa cgtcctaacg acggcgtacc gccggcccgg
     1561 ctcgggacgc gagtagtcga gcgcatcgcg gaatccatac gatccgag
//
LOCUS       DASUCJ010001229         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1266315, whole genome shotgun sequence.
ACCESSION   DASUCJ010001229 DASUCJ010000000
VERSION     DASUCJ010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266315"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="VFU90_14370"
     CDS             complement(<1..1855)
                     /locus_tag="VFU90_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU5481027.1"
                     /translation="MLHATPRRAAVAAGAFLLFAAPCLTAQDSTQAKPTFVNGMGQVV
                     PAFADSSTWIRQDLWVETGFDSDQDGKPDRVHVDVTRPRQTDDGLKVAVIYGSSPYYA
                     GVSRDQVDWDVRQELDAKPKPRGLMLSAPYDGHRTRISSELVNEWVPRGFAVVHSEAP
                     GTGRSQGCPTVGDTPERTAMKYVIDWLNGRAKGYTTETGSEEVKATWSTGKVGMMGTS
                     YEGTLPLAAATTGVDGLEVVIPVSPNTSYYHYYRSNGLVRSPGGDLGEDVDVLYDFIA
                     SGDTAERATCERLYKNGIFAKGQDRVTGDYNDFWAKRDLLPWVKNIKAAVLLAHGLND
                     YNVVSEHSLRIYNEMKKDGLPVSLYLHQGGHGGNPPADMVNRWFSHYLYGVDNGVQKD
                     PPVWIVEDSASQAPQPIIAAAQAAQDSARRVEGSGGEPTPAAAPTGRRRRRPAGPPTP
                     FASFPVPGSSPVQFYPTGSGTAVGTLAFAAAPNGKGTLVDDVTSSGSADASAASSNHR
                     LVYASPVLTDTVHISGTPRVTLRIASSEPVANLSVWLVMLPYDSTVVGSQSRAGLISR
                     GWADPQNYRSLTSGGNYDSEQPGEPVVPGRFYDLTFDLEPDDEFVPAGKRFA"
BASE COUNT          315 a          724 c          706 g          349 t
ORIGIN      
        1 gcgcgaaccg cttcccagcc ggcacgaact cgtcgtcggg ctccaaatcg aacgtgagat
       61 cgtaaaacct gcccggcacc accggctcgc ccggctgctc cgagtcgtag tttccgcccg
      121 acgtgagcga tctgtagttc tgcggatcgg cccagccgcg cgagatgagc ccggcgcggc
      181 tctgtgagcc gacgacggtc gaatcgtacg gcagcatcac gagccacaca ctcagattcg
      241 ccaccggctc gctcgatgcg atgcgcaacg tgacgcgcgg cgtaccggag atgtgcacgg
      301 tgtccgtgag cacgggcgat gcgtacacca ggcgatggtt cgagctcgcg gcgctcgcgt
      361 cggcgctgcc gctcgaggtc acgtcgtcga ccagcgtacc ctttccgtta ggcgccgccg
      421 cgaaggcgag agtgcccacc gcggtgccgc tgccggtcgg atagaactgc accggcgacg
      481 atcccggcac ggggaacgac gcgaacggtg tcggcgggcc ggcgggccgg cgacgccggc
      541 gccccgtggg cgctgccgcc ggggtcggct cgccgccgct cccttccacg cgacgcgccg
      601 aatcctgcgc cgcctgcgcc gcggcgatga tcggctgcgg cgcttgcgat gcggagtcct
      661 ccacgatcca cacgggcgga tccttctgga cgccgttgtc gacgccgtac aggtagtggc
      721 tgaaccagcg gttgaccatg tccgccggcg ggttgccgcc gtgtccgccc tgatgcaggt
      781 acagcgagac gggcagccca tccttcttca tctcgttgta gatgcggagg ctgtgctcgg
      841 acaccacgtt gtagtcgttg agcccgtgag cgaggagcac cgcggccttg atgttcttca
      901 cccacggcag caaatcgcgc ttggcccaga agtcgttgta gtcgccggtg acgcggtcct
      961 gccccttcgc gaagatgccg ttcttgtaca gccgctcgca cgtcgcgcgc tcggccgtgt
     1021 cgccgctggc gatgaagtcg tacagcacgt cgacgtcctc gcccagatct ccgccgggcg
     1081 accgcacgag cccgttggac cggtagtagt ggtagtacga cgtgttaggc gaaaccggga
     1141 tcacgacctc gagcccgtcg acgccggtgg tcgccgccgc gagcggcagc gtgccttcgt
     1201 acgacgtgcc catcatcccg actttgccgg tggaccacgt ggctttcacc tcctcggagc
     1261 cggtctcggt ggtgtagccc ttggcgcggc cgttgagcca gtcgatcacg tacttcatgg
     1321 ccgtgcgctc gggcgtgtcg cctaccgttg ggcatccctg cgagcgcccc gtgcccggcg
     1381 cttcggagtg gaccaccgcg aatccgcgcg gcacccactc gttcaccaac tcgctcgaga
     1441 tgcgcgtgcg atgtccatca tacggcgcgg acagcatcag gccgcgcggc ttcggtttgg
     1501 cgtcgagctc ctggcgcacg tcccagtcga cctggtcgcg cgacacgccg gcgtagtagg
     1561 gactcgatcc atagatgacc gcgaccttga ggccgtcatc ggtctggcgc ggccgcgtga
     1621 cgtcgacgtg gacgcggtcc ggtttgccat cctgatcgct gtcgaagccg gtttcgaccc
     1681 acaggtcctg tcgaatccag gtgctggaat cggcgaacgc cggcacgacc tgccccatgc
     1741 cgttgacgaa cgtcggcttg gcctgcgtcg aatcctgcgc cgttaggcac ggggcggcga
     1801 acagcaggaa cgcgcccgcg gccacggcgg cgcggcgcgg agttgcatgc aacatggtca
     1861 caacggtgcg agacatggat gggcgcacgg tcgttcacgg atgcgaacca cgcgcgcgct
     1921 gagacgaagc gtcacgacgt gctgtcgtac gtcacgacgc gttggacggc ctccagctcg
     1981 tcctgcggcg gcgcgcccgc aatgggtgcg acggccgtgc cgaccgcgaa gaattcgatc
     2041 gcgttggagc cccagcccca gttcgactct tcgacgcgcg cgccgacaac gccg
//
LOCUS       DASUCJ010001230         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1495670, whole genome shotgun sequence.
ACCESSION   DASUCJ010001230 DASUCJ010000000
VERSION     DASUCJ010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495670"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFU90_14375"
     CDS             complement(<1..1055)
                     /locus_tag="VFU90_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5481028.1"
                     /translation="MPFDPTTKRAEAIVRRGGVEIHIVKGAPQVVGALLGVKSDFAAA
                     AELLAADGARVLAVASGPAGQLGLAGLISFTDPIRDDSKTLVKTLRSRGVRVVLVTGD
                     GAATARAVGRVLGIGDRVCGHEEAARDALSCDVIAGVLPEDKLRVVRALQRTGAVVGM
                     TGDGVNDAPALKQAEIGIAVESATDVAKAAASIVLTRPGLEGVLAAVETGRRIYQRML
                     TYTINKIVKTIEVALFLSIGFVLTGELVTSPRLILLLLFTNDFVTMSLAGDRVTGSAE
                     PERWNVRFLVTAALWLGVAWVVFSLAVFFVGRDVVRLPHASVQTLSFLVLVFGGQANV
                     YSVRERRRFWQSTPSPW"
BASE COUNT          216 a          360 c          342 g          174 t
ORIGIN      
        1 atccacgggc tgggcgtcga ttgccaaaat cgacgccgct cgcgcaccga atagacgttt
       61 gcctgacctc cgaacacgag caccagaaat gacaaggttt gtaccgaggc atggggaaga
      121 cgaacgacgt cacgcccgac gaagaatacg gcgagcgaaa agacgaccca ggccacgccc
      181 agccacagag ccgcggtgac gaggaagcgc acgttccatc gctcgggctc ggccgagccg
      241 gtcacgcggt cccccgcgag cgacatggtg acgaagtcat tcgtgaacag caagagcaag
      301 atcagacgtg gcgaggtcac gagctccccc gtcaggacga acccgatgct caaaaagagc
      361 gcgacctcga tcgtcttcac gatcttgttg atcgtgtagg taagcattcg ttgataaatg
      421 cgccgccccg tctctacggc cgcgagaacg ccctcgagtc ccggccgagt gagcacgata
      481 ctcgcagcgg ccttggcaac gtcggttgca ctttcgacgg cgatgccgat ctcggcctgt
      541 ttcagcgcag gtgcgtcgtt cacgccatcg cctgtcatgc ctacgacggc gccggtgcgt
      601 tgaagtgcgc gcacgacgcg aagcttgtct tcgggcaaga cgccggcaat gacgtcgcat
      661 gatagcgcgt cgcgagcggc ctcttcgtgc ccgcacactc gatcgccgat cccgagtaca
      721 cggccgacgg cccgcgccgt cgctgcacca tcgccggtga cgagcaccac gcgcacgccg
      781 cgggagcgga gagttttcac cagtgtcttc gagtcgtccc ggatggggtc cgtgaaactg
      841 atcaatccgg cgaggcctag ctgaccagcc ggaccggatg cgacagccaa tacgcgcgcg
      901 ccgtccgcgg cgagaagttc cgccgccgcc gcgaaatcac ttttgacgcc gaggagagct
      961 ccgacgacct gcggggcgcc tttcacgatg tggatctcga cgccgccgcg ccgaacgatc
     1021 gcctcggcgc gcttcgtcgt cgggtcgaat ggaatgaacc gtagccgctc cgtagcctgg
     1081 gattcctcgt gg
//
LOCUS       DASUCJ010001231         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1021790, whole genome shotgun sequence.
ACCESSION   DASUCJ010001231 DASUCJ010000000
VERSION     DASUCJ010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021790"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFU90_14380"
     CDS             complement(<1..333)
                     /locus_tag="VFU90_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5481029.1"
                     /translation="MSGRVPWWRRYQELPVPELQRSNPLRFGLITIVLLAIVLYFGFT
                     KHIPGTHGFRLKAQFGTVVNIAPKSPVRIAGVPVGKVTSIQRDGDTGVVTMEISKAGL
                     PIHRDATAK"
     gene            complement(330..>1050)
                     /locus_tag="VFU90_14385"
     CDS             complement(330..>1050)
                     /locus_tag="VFU90_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481030.1"
                     /translation="EFVNKSAEFMRLLRPSAQILRTVAAPLGHAFQVGAVNIRAATAL
                     APVVRESSEALAEFAKNPIVTTSLEQLTRTLGYANPLLAGLAPAQRNCNYLTLTFRNL
                     ASVLSESVGVGTLGRAAAVLAPDGPNNEGVAASAPANGPSVDHGPPNEINGVKIPGAA
                     VDNNHLHYNAYPFVGSSGQPNVCEAGNEPFIAGKAVIGNVPATSRAHDETTRKTDMFG
                     ETYPRSTLEALGIPGSKGKSK"
BASE COUNT          140 a          336 c          377 g          197 t
ORIGIN      
        1 cttcgcggtc gcgtcgcgat gaatcggcag gccggccttg gagatttcca tcgtcacgac
       61 tcccgtgtcg ccgtcgcgct ggatcgaggt gaccttgccc accgggacgc cggcgatgcg
      121 cacgggcgat ttcggcgcga tgttgaccac tgtcccgaac tgggccttca gcctgaaccc
      181 gtgcgtgccg gggatgtgct tggtgaagcc gaagtacagg acgatcgcca gcagcacgat
      241 cgttatcagg ccgaagcgca gcgggttcga gcgttgcagc tcggggaccg gcagctcctg
      301 gtatcgccgc caccacggca cgcgcccgct catttgctct ttcctttcga gccgggaatg
      361 ccgagcgctt cgagcgtgga gcgcgggtac gtttcgccga acatgtcggt cttgcgggtg
      421 gtttcgtcgt gcgccctgct cgtggccggc acgtttccga tcacggcctt gcccgcgatg
      481 aagggctcgt tgccggcttc gcaaacgttc ggctggcccg aggagccgac gaacgggtag
      541 gcgttgtagt gcaggtggtt gttgtcgacg gccgcgccgg gaatcttcac gccgttgatt
      601 tcgttcggcg gcccgtgatc gaccgacggg ccgttggccg gcgccgaggc ggcgacgccc
      661 tcgttgttgg ggccgtccgg agcgagcacc gccgcggccc tgccgagcgt gccgacgccg
      721 acgctctcgg acagcacgct cgccaggttg cggaacgtca gcgtcaggta gttgcagttt
      781 ctctgcgccg gtgccaggcc cgccagcagc ggattggcgt agccgagcgt gcgcgtgagc
      841 tgttcgaggc tagtcgtgac gatcgggttc ttggcgaatt ccgcgagcgc ttccgagctt
      901 tcccgcacca ccggggccag cgccgtggcg gcgcggatgt tgacggcgcc cacctggaag
      961 gcgtgtccga gcggggccgc gaccgtgcgc aggatctgcg cgctcgggcg cagcaggcgc
     1021 atgaactcgg cgctcttgtt gacgaattcg
//
LOCUS       DASUCJ010001232         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_89521, whole genome shotgun sequence.
ACCESSION   DASUCJ010001232 DASUCJ010000000
VERSION     DASUCJ010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89521"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1282
                     /locus_tag="VFU90_14390"
     CDS             236..1282
                     /locus_tag="VFU90_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5481031.1"
                     /translation="MLPADGPTDCALPAGSSIAPGLIDVHTNGAGDALFNRDQGNAVT
                     TAAVEYARMGATGFVAGVMTAPFESMLHAASEIVEAANQLEEDEPRGARCLGVHFEGP
                     FLNSKFRRVHRAEWLLPASTARAREMIDACKGACLLVTMAPEVDGASDALRVFLENGV
                     VCSAGHTSARYSEGLLAIGLGFRSLTHAFNGMPPLDHRDPSILAAFIQDRRTLVQLIC
                     DGFHVSPVMVDLLHRTLGDRVVLATDNMPPADPSYHIAGGVMRAADGTIAGSALRIDE
                     AVRNYMSYAQIPFAQAIVAATYAPAKLIHHDAEIGRIALRMRADLSFWDQDYRIVGTM
                     VGGRFVYSRAEVAA"
     gene            complement(1255..1446)
                     /locus_tag="VFU90_14395"
     CDS             complement(1255..1446)
                     /locus_tag="VFU90_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481032.1"
                     /translation="MRAALGLTLGGIPGVLIAAYLVKSLPLSAVRWLVVGVVVYAAVV
                     MLRSAAQRATAYAATSARL"
     gene            1466..>1658
                     /locus_tag="VFU90_14400"
     CDS             1466..>1658
                     /locus_tag="VFU90_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481033.1"
                     /translation="MKRRLATDNMPPADPSYHIAGGVMRAADGTIAGSALRIDEAVRN
                     YMSYAQIPFAQAIVAATYAP"
BASE COUNT          296 a          594 c          523 g          245 t
ORIGIN      
        1 ggccgaatcg accggcaagc gcggattctt cgggcggttg ttcggcaaag ggcgatgacc
       61 ggcgcaacgt cgaacgccat cgtccgcggg gacgctccca cgcacgcctc acgcgtcgaa
      121 ggagtacacg agaacgtcta acggcccttc ggtactagga cccgccttca tcgccgtcgg
      181 cgacggtacg tcgaccttcg gacgcatcgc aattgacgac ggacggatcg ccgcggtgct
      241 gccggccgac ggacccaccg attgcgcgtt acccgccggg agcagcatcg cgcccggcct
      301 gatcgacgtt cacaccaacg gtgccggcga cgcacttttc aatcgcgatc aaggcaacgc
      361 ggtcacgacc gcggccgtcg aatacgcgcg catgggcgcg accggattcg tcgccggcgt
      421 gatgacggcg cccttcgagt cgatgctgca cgcggcatcc gagatcgtcg aagcggccaa
      481 tcagctcgaa gaggacgaac cgcgcggcgc ccgctgcctg ggcgtgcact tcgaaggccc
      541 gttcctcaac tcgaaattcc ggcgcgtgca ccgcgcggag tggctgctgc cggcgagcac
      601 ggcgcgcgcg cgagagatga tcgatgcctg caaaggcgcc tgtctgctgg tgacgatggc
      661 gcccgaagtc gacggtgcga gcgacgcgct gcgcgtgttc ttggaaaacg gcgtcgtctg
      721 ctcggccggg cacacgtcgg cccgctacag cgagggtttg ctggccatcg gcctcggctt
      781 ccgctcgctg acccacgcct tcaacgggat gccgccgctg gatcaccgcg atccgtcgat
      841 tctggcggcc ttcatccaag accggcgcac tctcgtccag ctgatttgcg acggtttcca
      901 cgtctcgccg gtcatggtcg atctgctgca ccgcacgctc ggcgaccggg tcgtgctcgc
      961 gaccgacaac atgccgcccg ccgatccgag ctatcacatc gccggcggtg tgatgcgcgc
     1021 cgccgacgga acgatcgccg gcagcgccct gcgcatcgac gaagccgtcc gcaactacat
     1081 gtcgtacgcg caaatcccgt tcgcgcaagc gatcgtcgcc gccacctacg cgccggccaa
     1141 gctcatccat cacgacgccg agatcggccg catcgcgctg cgcatgcgtg ccgacctctc
     1201 gttctgggat caggactacc gcatcgtcgg aacgatggtc ggcggccgct tcgtctacag
     1261 ccgcgccgaa gtcgcggcgt aagccgtcgc tcgttgcgcc gcggatcgaa gcatcacgac
     1321 ggcggcataa acgaccacgc cgacgaccag ccatcgaacg gccgataagg gcagcgactt
     1381 taccaggtac gcggcgatca gcacgcccgg gatccctccg agcgtcaaac cgagcgcggc
     1441 gcgcatcgaa aaagcatcga aacgaatgaa gcgcaggctc gcgaccgaca acatgccgcc
     1501 cgccgatccg agctatcaca tcgccggcgg cgtgatgcgc gccgccgacg gaacgatcgc
     1561 cggcagcgcc ctgcgcatcg acgaagccgt ccgcaactac atgtcgtacg cgcaaatccc
     1621 gttcgcgcaa gcgatcgtcg ccgccaccta cgcgccgg
//
LOCUS       DASUCJ010001233         1224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1220743, whole genome shotgun sequence.
ACCESSION   DASUCJ010001233 DASUCJ010000000
VERSION     DASUCJ010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220743"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..>1224
                     /locus_tag="VFU90_14405"
     CDS             36..>1224
                     /locus_tag="VFU90_14405"
                     /inference="COORDINATES: protein motif:HMM:NF044554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481034.1"
                     /translation="MPGDPLAIAAAARTFDSIAAEIASTSAKLRALATANSHWTGNAA
                     SQAKTRTITLPPKLDKAQRSYATAGGALRNYANALDDAQNRSQAAARSADVANADLLA
                     ARASQQSAVANDASRARAAAAAGLPTPQPTAPLYDTEISDAEARLANAVRANTDAHND
                     LRRAARTAAAQLHEASREGIKNQPWWRHVLSGAARFASSSWTTSLRVVSRVATAISAL
                     AGLAALAVSVVGLVFPPLEGAAAVLETISLVSGVMATCADAVLAASGNGSWTSVGADA
                     TVLAPWAGSRFVNKTVRLLKNPTRVAASTGSFEEPGNLRAAYPVFVGKGVRTRSINYR
                     PRSTDPNWGLRGIHLDKHLFGNGASSLAVLDPAGNPDRWISLVQALATMPTTSSPEPG
                     IDDI"
BASE COUNT          233 a          427 c          370 g          194 t
ORIGIN      
        1 agcgtcgtcg cgtgccaacc agcgagctgc agccgatgcc cggcgaccca ctagcaatag
       61 ccgccgcagc tcgcacgttc gactcaatcg ccgcggaaat cgcctccacg tcagcgaaac
      121 tccgcgcact cgcgacggcc aactcacact ggacgggaaa cgcggccagc caagccaaga
      181 cgcgcaccat cacgctgcca cccaaactcg acaaggcgca gcggtcgtac gcgaccgcgg
      241 gcggtgcact ccgcaactac gccaatgcgt tggacgacgc gcagaaccgt tcgcaagctg
      301 ccgcacgttc ggcggacgtc gcaaacgcag acctcttggc cgcccgcgca tcgcaacaat
      361 cagccgtcgc caatgacgct tcgcgagcaa gagcggcagc agcggccggt ctcccgacac
      421 cgcaaccaac cgcgcccctt tacgacaccg agatatccga cgccgaagcg cgcctcgcga
      481 acgccgtccg agcaaacacc gatgcgcaca acgatttgcg ccgcgccgcg cgaaccgccg
      541 cggcgcaact tcatgaggca tcgcgcgagg ggatcaagaa tcaaccgtgg tggcgtcatg
      601 tcctgagcgg cgcggcacga ttcgcctcat caagttggac gacgtcgctg cgcgtcgtat
      661 cgcgcgttgc gaccgccatc tcggcgctcg ccgggcttgc cgcgctagcg gtcagcgtcg
      721 tcgggctggt ctttccgcca ctggaaggcg cggccgcggt gcttgagacg atttcgctgg
      781 tcagcggagt catggcaacg tgcgccgacg cggtgttggc ggcgtccgga aacggctcgt
      841 ggacgtcagt cggcgccgac gcgaccgtcc tcgcgccatg ggctgggtcg aggttcgtta
      901 acaagactgt tcggctgctc aagaatccga cacgggtcgc cgcaagcaca ggatcttttg
      961 aggaacccgg caatcttcgc gccgcgtatc cggtctttgt cggcaaaggt gtccgcaccc
     1021 gctcgatcaa ctatcggcca cgttcaactg atccgaactg gggcctgcgc ggaattcact
     1081 tggacaagca cctattcggc aacggtgcat cttcccttgc ggtgctggat ccggccggca
     1141 acccagatcg atggatctcc cttgttcaag ctcttgcgac tatgccgacg acgtcgtcgc
     1201 cagagccggg catcgatgac atcg
//
LOCUS       DASUCJ010001234         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1189618, whole genome shotgun sequence.
ACCESSION   DASUCJ010001234 DASUCJ010000000
VERSION     DASUCJ010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189618"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFU90_14410"
     CDS             <1..259
                     /locus_tag="VFU90_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481035.1"
                     /translation="DLNLPPRTALACMSETMVLALEGRFEPYTLGRGIELAKVIEMMA
                     MSERCGFGLADMRAFDAAITPERIAEIRAAAERRRRQEAIA"
     gene            256..1161
                     /locus_tag="VFU90_14415"
     CDS             256..1161
                     /locus_tag="VFU90_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481036.1"
                     /translation="MKKVVSVSLGSSTRDHRSVVTLMGEEFDISREGTDGKLDVAIAK
                     VRELDGKVDAIGLGGIDVYLYAGRHRYALRDGLRLLEAAKITPVVDGSGLKNTLEREA
                     VRFLREDLGVDVRGRKVLMVSALDRFGMAQALVEAGADVLFGDFIFALDKDLPVRDLA
                     TFEQMAEKYLPDACKLPFQFFYPTGKKQEKPPEPKYPQYYEEADIIAGDFHFMRQFMP
                     DRLDGKIVLTNTVTPRDVDDLAERGVRMLVTTTPDFGGRSYGTNVIEAALLALLGKKW
                     SEVTADDYQRVLHELDLKPRVVSLN"
BASE COUNT          256 a          421 c          456 g          213 t
ORIGIN      
        1 cgacttgaac cttccgccgc ggacggcgct cgcgtgcatg agcgaaacca tggtcctggc
       61 gctggaggga cggttcgagc cgtatacgct cggccgcggc atcgaactcg ccaaagtcat
      121 cgagatgatg gcgatgagcg agcgctgcgg cttcgggctg gccgacatgc gcgcgttcga
      181 cgcagcgatc acgcccgaac ggatcgccga aatccgcgcg gcggcggagc gccgccgccg
      241 ccaggaggcg atcgcgtgaa gaaggtcgtt tcggtgtcgc tgggttcttc gacgcgcgac
      301 caccggtcgg tcgtgacgct gatgggcgag gagttcgaca tctcgcgcga aggcaccgac
      361 ggcaagctgg acgtcgcaat cgcgaaggtc cgcgaactcg acggaaaggt cgacgccatc
      421 ggcctgggcg gtatcgacgt ctatctctac gcagggcgcc atcgctacgc gcttcgcgac
      481 ggactgcgcc tgctggaggc ggccaagatc acgccggtgg tcgacggcag cggcttgaaa
      541 aacacgctgg agcgcgaggc cgtccggttt ctgcgcgaag atctcggcgt ggacgtgcgc
      601 ggccgcaagg tgctgatggt gagcgcgctc gaccggttcg ggatggcgca ggccctggtc
      661 gaagccggcg ccgacgtgct gttcggcgac ttcatcttcg cgctcgacaa agatctgccg
      721 gttcgcgacc tcgcgacctt cgagcaaatg gcggagaagt atcttcccga cgcgtgcaag
      781 ctgccgtttc agttcttcta cccgacgggg aagaaacaag agaagccgcc cgagccgaaa
      841 tacccgcagt attacgaaga agccgacatc atcgccggcg atttccactt catgcgccag
      901 ttcatgcccg accggctcga cggcaagatc gtgctcacca atacggtcac gccgcgcgac
      961 gtcgacgacc tcgccgagcg cggggtgagg atgctggtga cgacgacccc cgatttcggg
     1021 gggagatcct acggcacgaa cgtgatcgag gccgcgcttc tggccctgct cgggaaaaaa
     1081 tggagcgagg tgacggcgga cgactatcag cgcgtcctgc acgaactcga cttgaagccg
     1141 cgggtggttt ccctcaacta gaggtttcct aacgcccgcc ggggagcgag cgtacgggcg
     1201 ccccgaagcg ccggctgccg cccgagaagg tgaaggtttt caccccccga agaacccgcg
     1261 ccagcgcccg caagggcgtg tcatcttatg agggtccaca gttggacgat aaagagatcg
     1321 tcctcaccgc cgaaggactg gcgaag
//
LOCUS       DASUCJ010001235         1240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1189638, whole genome shotgun sequence.
ACCESSION   DASUCJ010001235 DASUCJ010000000
VERSION     DASUCJ010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189638"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFU90_14420"
     CDS             <1..375
                     /locus_tag="VFU90_14420"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5481037.1"
                     /translation="VLDLQIESETYPIQAEGLRADFARNDQVPFLDVVATRDAAGPVS
                     VFILNRDLDSERELTLEWQGPPPARVLASETLTGPDLKAFNTFEKPAQVSPQSFDAPK
                     PGVRMTFRVPARSYTVLQLAMS"
     gene            575..>1240
                     /locus_tag="VFU90_14425"
     CDS             575..>1240
                     /locus_tag="VFU90_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEU5481038.1"
                     /translation="MIENRSFEDPQQLYGWSEVNGGEGTFTVETESPLNANNPHFLRI
                     RADGSSYEFGVANDAFGGINLREGERYFFSVYARRRAGSENQSLKVYLAGEHGVLARA
                     ELKGLESSWKKFEATLTSSGTTTKGEFSLLVTQEGVVDVDMVSLFPEKTFKSRRNGLR
                     ADLAQALADAKPGFLRFPGGCIVEGRRLVNRYRWKTTIGDIAERQTIVNRWNDEFDHR
                     PTPD"
BASE COUNT          227 a          421 c          365 g          227 t
ORIGIN      
        1 gtgctcgacc tccagatcga atccgagacg tatcccatcc aggccgaggg attgcgcgcc
       61 gacttcgccc ggaacgacca ggtcccgttc ctcgatgtcg tcgccacgcg cgacgccgcc
      121 gggccggtga gcgttttcat cttgaaccgc gacctcgatt ccgagcgcga gctcacgctc
      181 gaatggcagg gcccgccgcc ggcgcgcgtg ctggcgtcgg aaaccttgac cggcccggac
      241 ctgaaagcct tcaacacgtt cgagaaaccg gcgcaggtct ccccgcaatc tttcgatgct
      301 cccaaacccg gcgtccggat gacgttccgg gtgccggcgc gttcgtacac ggtcctccag
      361 ctcgcgatgt cgtagaactt ccacgattat gaaatccaga acacttgctt tcgcctttgc
      421 cgccctgaat ctctgcgccg cgccgctctt cgccgccggc ccgccgcaaa tcgtcgttga
      481 caccgccgcg cccggaatcc gtacgccgcc gacgctgcac ggactatttt tcgaggacat
      541 caactacgcc gccgacggcg ggctgtatgc cgaaatgatc gagaatcgtt cgttcgagga
      601 tccccaacag ctttacggct ggtcggaggt gaacggcggc gagggaacgt tcaccgtcga
      661 aaccgagtcg ccgttgaacg cgaacaatcc gcactttctc cgcatccgtg cggacggcag
      721 ctcctatgag ttcggcgtcg ccaacgacgc cttcggcggc atcaatctcc gcgaaggcga
      781 gcgttacttc ttttccgtct acgcacggcg ccgcgccgga agtgaaaacc agtctctcaa
      841 ggtctatctc gcgggtgagc acggtgtttt ggccagggcc gagttgaagg gactcgaatc
      901 ctcctggaaa aaatttgaag ccacgttgac cagttccggc acgacgacga agggcgagtt
      961 ctcgctgctc gttacgcagg agggcgtcgt ggacgtggac atggtctcgc tgtttccgga
     1021 gaagacgttt aaaagccgtc gcaatgggct gcgcgccgat ctcgcccagg cgctcgccga
     1081 cgcgaagccc ggtttcctcc gcttccccgg cggctgcatc gtcgaaggcc gccgcctggt
     1141 gaaccgctac cgctggaaga ccacgatcgg cgacatcgcc gagcgccaga ccatcgtcaa
     1201 tcgctggaac gacgagttcg accatcggcc caccccggac
//
LOCUS       DASUCJ010001236         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_456520, whole genome shotgun sequence.
ACCESSION   DASUCJ010001236 DASUCJ010000000
VERSION     DASUCJ010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456520"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFU90_14430"
     CDS             <1..807
                     /locus_tag="VFU90_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5481039.1"
                     /translation="PDPDRRELDAALANVVESLEELRRTGEAGRLVREGLHAAIVGPP
                     NAGKSSLLNALLGQERAIVSELPGTTRDTIEESVTVGGVLVRLIDTAGIRSHADRLEA
                     AGIERTRRALADARLAIVVIDGSRPLGGDEHAILAATSGRARVVFANKADLGDAGARE
                     LEGTGAVSGSVRHGESLAALRSAIAAAGWGGELPDLERPHLAALREFDAVAEALDALG
                     RARATLGGGDPIDLATAELQRAFSALGHVSERIAAEEVLDGVFSRFCIGK"
     gene            828..1208
                     /locus_tag="VFU90_14435"
     CDS             828..1208
                     /locus_tag="VFU90_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481040.1"
                     /translation="MKRTLPVIVAALLVLSLSAGARAKLGQGFGVNIQSCVVNNNGGK
                     TNGINVVYSNLNASAATEVDFLVKYHKQRGVFADRGSFAQHALINHNLTNALVGIEWN
                     GPKPTLCKVQRVVLADGTIVTPSP"
     gene            1239..1802
                     /locus_tag="VFU90_14440"
     CDS             1239..1802
                     /locus_tag="VFU90_14440"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU5481041.1"
                     /translation="MVHVLARNWWALLIRGIAAVIFGILAFVWPAPTLLAIGILFGAY
                     AFVDGVFAIVAAVRAAQAHERWWPFVLEGIVGLAIAAITVYDVGITLVALYLTIAAWA
                     FITGILEIAAAIQLRKTIANEWLLILAGLCSILFGVLMIWHPMAAALAIIWIIAAYAI
                     VFGIIMIALAFRLRAHAPAAPATQAPA"
     gene            1832..>2448
                     /locus_tag="VFU90_14445"
     CDS             1832..>2448
                     /locus_tag="VFU90_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5481042.1"
                     /translation="MIVVGGGHAGVEAALAAARIGAETLLVTGDPEKICTLPCNPSIG
                     GSAKGQLVREIDALGGAMGRLADRCSLHARFLNESKGPALRALRQQMDKPAYARLAVE
                     ELRGQPRLSIATGLVETLIVEGGEVRGVACADGTRYFARRVVLATGTFLGAKTFRGSV
                     VAAEGRHGEAPAVGLAHALRRLGFPTRRLKTGTPPRIDKTTIDAEA"
BASE COUNT          384 a          824 c          850 g          390 t
ORIGIN      
        1 cccgatcccg atcgccgcga gctggacgcc gccttggcga acgtcgtgga atcgctcgag
       61 gagctgcgcc ggacgggcga agccgggcgg ctggtgcggg aaggcctcca cgcggcgatc
      121 gtcgggccgc ccaacgcggg aaaatcttcg ctgctcaacg cgctgctcgg gcaagagcgg
      181 gcgatcgttt cggagttgcc cggcaccacg cgcgacacca tcgaggaatc ggtcaccgtc
      241 ggcggcgtgc tggtgcgcct gatcgacacc gcgggcatcc gcagccacgc ggaccggctc
      301 gaagcggccg gcatcgagcg cacccggcgc gcgctcgcgg acgcccggct ggcgatcgtc
      361 gtgatcgacg gctcgcgtcc gctgggcggc gacgagcacg cgatcctggc cgcgacctcc
      421 ggccgcgccc gggtggtctt cgccaacaaa gccgacctgg gagacgccgg cgcccgggaa
      481 ctggaaggca ccggcgcggt gagcggcagc gtgcggcacg gcgaatcgct ggcggcgctg
      541 cgatccgcga ttgccgcggc cggctggggc ggcgagctcc cggacttgga gcgcccgcac
      601 ttggcggcgt tgcgcgagtt cgacgccgtc gccgaggccc tcgacgcgct cggtcgagcg
      661 cgcgcgacgc tcggcggcgg ggatccgatc gatttggcga ccgccgaact ccagcgcgct
      721 ttctctgccc tcgggcatgt aagcgaacgt atcgccgccg aagaagtgct cgacggcgtt
      781 ttcagccgtt tttgcatcgg aaaatgaacg aacgaacgga gggcgcaatg aaacggactc
      841 tcccggtcat cgtcgcggcg ctactcgtac tttcgctatc cgcgggcgcg cgggcgaaac
      901 tcggccaagg attcggcgtc aacattcaga gctgcgtcgt gaacaacaac ggcggcaaga
      961 ccaacggcat caacgtcgtc tacagcaacc tcaacgcgag cgcggcgacc gaggtcgact
     1021 tcctggtgaa atatcacaaa cagcggggcg tattcgccga ccgcggctcg ttcgcacagc
     1081 acgcgctgat caaccacaac ctcaccaacg cgctcgtcgg catcgagtgg aacggcccca
     1141 aaccgaccct ctgcaaggtc cagcgcgtcg tgctcgccga cggtacgatc gtcaccccct
     1201 caccgtagca gcttgccgtt accgatagga gtttagcgat ggttcacgtt ctcgcgcgca
     1261 actggtgggc gctcctgatc cgcggcatcg ccgcggtgat cttcgggatt ctggcgttcg
     1321 tttggccggc gccgaccttg ctcgcgatcg gcatcctgtt cggcgcgtac gcgttcgtcg
     1381 acggcgtgtt tgcgatcgtc gcggcggtcc gcgccgcgca ggcgcacgaa cggtggtggc
     1441 cgttcgttct cgaggggatc gtcggcttgg ccatcgccgc catcaccgtg tacgacgtcg
     1501 gcatcacgct ggtcgcgctc tacctcacga tcgccgcgtg ggcgttcatc accggaattc
     1561 tggagattgc cgcagcgatt caactgcgca agacgatcgc gaacgaatgg ctgctgatcc
     1621 tcgccggctt gtgttcgatt ctcttcggcg tgctgatgat ttggcatccg atggcggcgg
     1681 cgctggcgat catttggatt atcgccgcgt acgcgatcgt tttcggcatc atcatgatcg
     1741 cgctggcgtt tcgcctgcgc gcgcacgcgc cggccgcccc ggcgacccaa gcacccgcct
     1801 agcgaaaacg gtgcggcggg acgatgcggc ggtgatcgtc gtcggcggcg gtcacgccgg
     1861 cgtcgaagcc gccttggccg cggcgcggat cggtgccgaa acgctgctgg tcaccggcga
     1921 tccggagaag atctgcacgc tgccctgcaa tccgtcgatc ggcggaagcg ccaaaggcca
     1981 gctggtgcgc gagatcgacg cgctcggcgg cgcgatgggg cggctggcgg accgctgcag
     2041 cctgcacgcg cggtttctca acgagagcaa ggggccggcg ctccgggcgc tgcgccagca
     2101 gatggacaag cccgcctacg cgcgcctggc cgttgaggag ctgcgcggac aaccgcggct
     2161 ttcgattgcg accgggctgg tcgagacgtt gatcgtcgag ggcggcgagg ttcgaggcgt
     2221 agcatgcgcg gacggaacgc gctatttcgc gcggcgcgtc gtgctcgcga ccggcacgtt
     2281 cctgggcgcg aaaacctttc gcggaagcgt cgtcgccgcc gaaggccgtc acggcgaagc
     2341 gcccgccgtc gggctcgcgc acgccttgcg aaggctcggg tttcccaccc ggcgactgaa
     2401 aaccggtacg cccccgcgga tcgacaaaac gacgatcgat gccgaggc
//
LOCUS       DASUCJ010001237         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_976224, whole genome shotgun sequence.
ACCESSION   DASUCJ010001237 DASUCJ010000000
VERSION     DASUCJ010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976224"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFU90_14450"
     CDS             <1..486
                     /locus_tag="VFU90_14450"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5481043.1"
                     /translation="VPDFPIPGILFRDITPLLKDKQGFHQAIELFVERFKDRAVDYVV
                     GVEARGYIFGAPLAYAIGAGFIPIRKPGKLPHDTLSEEYALEYGTNALEVHADSLSHG
                     EHVVVVDDLLATGGTAAATRRLLERLGATVDAFAFLIELVNLKGRDALPGIDVVSFVR
                     Y"
     gene            489..974
                     /locus_tag="VFU90_14455"
     CDS             489..974
                     /locus_tag="VFU90_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481044.1"
                     /translation="MPRFVIAALLALGIASAAGIRPAAAGRPTPTPAPTPTPVADPAV
                     TRIARQQFVAWQAGSINKSLYAPEVVSKLTDAKIADVSHALSALGPLTGTVYIGPFSA
                     VDIPPDAHAYIYQMQCADGNIYLWMILDGKGKIATIFFKDKLDVETVERPGATASSPP
                     S"
     gene            1026..>3097
                     /locus_tag="VFU90_14460"
     CDS             1026..>3097
                     /locus_tag="VFU90_14460"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5481045.1"
                     /translation="MTIQELIDRVHQYDPSADGAWLTRVYEVADAAHRGQRRASGESY
                     IEHPLAVAGVLAELEMDRETIAAALLHDVVEDTSVTSEQVAAQFGEEIAALVEGVTKL
                     TRIPYQSKEDAQVENLRKMFMAMAKDIRVIIIKLADRLHNMRTLASLPLAKQQAIARE
                     TLDIYAPIAHRLGIWKIKWEIEDDCLRYLDPEAYREIVELVAKTRHEREADVQEATGR
                     LRADFKSMKINAEIQGRPKHFYSIYTKIKGGRDFSTIYDLTAIRIIVDTVKDCYAALG
                     AVHARWTPLPGRFKDYIAMPKPNMYQSLHTTVVGPTGEPLEIQIRTWDMHRTGEYGIA
                     AHWRYKEGGKVDQFENKLSWLRALLEWQKDMRDSRVFMESLKLDLFDSQVFVFSPRGD
                     VFSMPSGGTPLDFAYQVHTDVGNHCVGAKVNGRIVPLDSQLNNGDICEILVNKTSGRP
                     SLDWLSIVKTSSAKHKIKQWFRKEQREENVLAGQEALEHELARAGVRVDAARGELLER
                     IANRLNYPTPTDLYAAIGFGDASALAVANRLRDETKSSNVVDLTKIGRKPAARRSTRK
                     SSGVRIAGIDDMLVRLSKCCSPVPGDPIIGYVTIGRGVSIHRADCPNVAYMNSTPERI
                     MQAQWTANDELTHAVEVEVEAEDRSQLLQDIMAVFAELKTQVSSVTARVRRDGLAVAS
                     LTIQIRDL"
BASE COUNT          595 a         1008 c          995 g          499 t
ORIGIN      
        1 gtcccggatt ttccgattcc cggaattctt tttcgtgaca ttaccccgtt gttgaaggat
       61 aagcaaggct ttcaccaggc aatcgagctg ttcgtcgagc gattcaagga ccgggcggtc
      121 gactacgtgg tcggcgtcga agcgcgcggc tatatttttg gagctccgct ggcgtatgcc
      181 atcggcgcgg gcttcatccc gattcgcaaa ccgggcaagc tcccgcacga tacgctcagc
      241 gaggagtacg cactcgagta cgggaccaac gcgctggaag tgcatgccga ttcgctcagc
      301 cacggcgagc acgtggtcgt cgtcgacgat ctgctcgcga ccggcgggac ggcggccgcg
      361 acgcgccgtc tgctcgagcg gctgggtgcg acggtcgacg cgtttgcgtt tctgatcgag
      421 ctggttaacc tgaagggacg cgacgcgttg cccggtatcg acgtcgtctc gttcgtgcgg
      481 tactgaccgt gccgcgattc gtaatcgccg cgctcttggc gctcggcatc gcaagcgcgg
      541 ccggcatacg gccggcggcg gccgggcggc cgacccccac cccggcgcct acgccgacgc
      601 cggtcgccga cccggcggtc acccggatcg cccgccagca gttcgtggct tggcaggccg
      661 ggagcatcaa caagagcctg tacgcccccg aggtcgtttc aaagctcacc gacgccaaga
      721 tcgccgacgt ttcgcacgcg ctttccgcgc tgggtccgct caccggcacc gtctacatcg
      781 ggcccttctc ggcggtcgac atcccgcccg acgcgcacgc gtacatctac caaatgcagt
      841 gtgcggacgg caatatctac ctctggatga tcctcgacgg caaggggaag atcgcgacca
      901 ttttcttcaa ggataagctc gacgtcgaga cggtcgagcg gccgggcgcg acggcgtctt
      961 cgccgccctc gtaacgaagc aactgacgtc tcgccgcact cgcggtataa tgggcggata
     1021 ccgccatgac cattcaggag ctgatcgacc gcgtccacca gtacgacccg tccgccgacg
     1081 gggcgtggct gacgcgcgtc tacgaggtcg ccgacgccgc tcaccggggt cagcggcggg
     1141 cctccggcga gtcgtacatc gagcacccgc tggccgtagc gggggtgctg gcggagctgg
     1201 aaatggaccg cgaaaccatc gccgcggcgc tgctgcacga cgtcgtcgag gacacctcgg
     1261 ttaccagcga gcaagtcgcg gcgcagttcg gcgaggaaat cgcggcgctc gtcgagggcg
     1321 tgaccaagct gacccggatc ccctatcaat cgaaggaaga cgcgcaggtc gagaacctcc
     1381 gcaagatgtt catggcgatg gccaaggaca tccgggtgat catcatcaag ctcgccgacc
     1441 ggctgcacaa catgcggacg ctggcgagtc tgccgctcgc caaacagcag gcgatcgcgc
     1501 gcgagaccct cgacatctac gcgccgatcg cgcaccggct cggcatttgg aagatcaagt
     1561 gggagatcga ggacgactgc ttgcgttatc tcgatccgga ggcctaccgc gaaatcgtcg
     1621 agctggtcgc caagacgcgc cacgagcgcg aggccgacgt gcaggaagcg accggccggc
     1681 tgcgcgccga tttcaagtcg atgaagatca acgccgagat tcaagggcgt ccgaaacatt
     1741 tctactcgat ttatacgaag atcaaaggcg gtcgcgactt ctcgacgatc tacgatctca
     1801 ccgcgattcg gatcatcgtc gataccgtca aggattgcta cgccgcgctc ggcgcggtgc
     1861 acgcgcgctg gacgccgctg cccggacggt tcaaagacta catcgcgatg ccgaagccca
     1921 atatgtatca gtcgctgcac acgacggtgg tcgggccgac cggcgagccg ctcgagatcc
     1981 agattcgcac ctgggacatg caccgtaccg gcgaatacgg catcgccgcc cactggcgat
     2041 ataaagaagg cggtaaggtc gaccaattcg agaacaagct gtcgtggctg cgcgcgctgc
     2101 tcgagtggca aaaagacatg cgcgactcgc gcgtcttcat ggagagcctc aagctcgacc
     2161 tgttcgactc gcaggtgttc gtcttctcgc cgcgcggcga cgtgttctcg atgccgtccg
     2221 gcggcacgcc gctcgacttc gcctatcagg tgcacaccga cgtcggaaac cactgcgtcg
     2281 gcgccaaggt caacggccgg atcgttccgc tcgattcaca gctcaacaac ggcgacatct
     2341 gcgagatcct ggtcaacaaa acgagcgggc ggccctcgct ggactggctg tcgatcgtca
     2401 agacgtcgag cgccaagcac aagatcaagc aatggttccg caaggaacag cgcgaggaga
     2461 acgtgctcgc cgggcaagag gcgctcgagc acgagctggc ccgcgccggc gtgcgcgtcg
     2521 acgcggcacg cggcgaactg ctcgaacgca tcgccaaccg gctgaactac ccgacgccga
     2581 ccgatctcta tgcggcgatc ggtttcggcg acgcctcggc cctggcggtc gccaaccggc
     2641 tgcgcgacga aaccaagagc agcaacgtcg tcgatctcac gaagatcggc cgcaaaccgg
     2701 ccgcccgtcg cagcacgcgc aaatcgagcg gagtgcggat tgcgggcatc gacgacatgc
     2761 tcgtgcggct ttcgaaatgc tgctcgccgg tgccgggcga cccgatcata ggctacgtca
     2821 cgatcggacg cggggtgagc atccatcggg ccgactgccc gaacgtcgcg tacatgaact
     2881 cgacgccgga acgcatcatg caggcgcagt ggacggccaa cgacgagctg acgcacgcgg
     2941 tcgaggtgga agtcgaagcc gaagaccgct cgcagctgct gcaagacatc atggcggtct
     3001 tcgccgagct gaaaacgcag gtgagctcgg tgacggcgcg ggtgcggcgc gacggcttgg
     3061 cggtcgcgag cctgacgatt cagatccgcg acctcga
//
LOCUS       DASUCJ010001238         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_273009, whole genome shotgun sequence.
ACCESSION   DASUCJ010001238 DASUCJ010000000
VERSION     DASUCJ010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273009"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFU90_14465"
     CDS             complement(<1..358)
                     /locus_tag="VFU90_14465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5481046.1"
                     /translation="MLLETSLGRILVDPMLRGAGTTPPIENTPNSVRNPLVELPVPAE
                     EVVRDVDLCIVTHLHGDHFDDAADALLPRGLPILTQPESAEALRARGFTNVATSHGKI
                     PMTRGHHGKGEIGAAMG"
     gene            complement(669..>1204)
                     /locus_tag="VFU90_14470"
     CDS             complement(669..>1204)
                     /locus_tag="VFU90_14470"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU5481047.1"
                     /translation="LGGAAERSFSMAMDALRRDDEHDTLLLIAHDCDGHARGYLHFVP
                     SFGRPAASLSLMRRDPETPNGLTEFMVVKAIEQLRARGIDEVSLNFAAFARFIHDPQG
                     LLERTAGRLARRADAVFQIERLYRFNAKFFPRWEPRYVMYERLAALPRVALASLWLEG
                     QIPKPTLPRVPREPARR"
BASE COUNT          176 a          374 c          451 g          203 t
ORIGIN      
        1 gccccatcgc cgcaccgatc tctcccttgc cgtggtggcc ccgcgtcatc gggatcttcc
       61 cgtgcgacgt ggcgacgttc gtgaacccac gtgcacgaag cgcctcggcg ctttccggct
      121 gtgtgaggat cggcagcccg cgcggcagca gcgcgtccgc cgcgtcgtcg aagtggtcgc
      181 cgtgcaggtg cgtgacgatg cacaggtcga cgtcgcggac gacctcctcc gcgggcaccg
      241 gcagctcgac gagcgggttg cggacggagt tcggcgtgtt ctcgatcggc ggcgtcgtgc
      301 ccgcgccgcg cagcatcggg tcgacgagga tacggcccag gcttgtctcc agcagcatcg
      361 tcgcgtggcg gacaagcctg atccgcacgt cagccgcgcc gtggtctcgg cgacgtcgcg
      421 cgaactgttc gccgaaggaa gctgaggctg gttcgcatga cctcagcgta cgtccagcaa
      481 tcgatgcgcc ggaacgcact cggctgggcc agcgttcggg aatgttcccc gagcgcagca
      541 gacgccggct catccgcccg gcgcgaggag cccgtcgcgc tcggcgccct cgacggcagc
      601 gagacgatcc ctggcggcct gatgccggtg ccgccttggg tcgagggatg aacgtgaccg
      661 ttgggatatc agcgtcgagc tggttcgcgt ggaacgcggg gcagcgtcgg ctttgggatc
      721 tgtccttcga gccagagtga ggcgagtgcg acgcgcggta gagcggcgag gcgctcgtac
      781 atgacatagc ggggctccca gcgggggaag aacttggcgt tgaagcggta gaggcgttcg
      841 atctggaaga ccgcgtcggc gcgtcgggcc agccgcccgg cggtacgttc gagcaggcct
      901 tgcgggtcgt ggatgaatcg tgcgaaagcg gcgaagttga gtgagacctc gtcgatgccg
      961 cgcgctcgca gctgttcgat tgccttgacg accatgaact cggtcaggcc gttgggtgtt
     1021 tccgggtcgc ggcgcatcag cgagagtgat gcggccggcc ggccaaagct gggtacgaag
     1081 tggaggtagc cgcgcgcgtg accgtcgcag tcgtgggcga ttaggagcag tgtgtcgtgt
     1141 tcgtcgtcgc ggcggagggc gtccatggcc atcgagaagc ttcgctcggc tgcgccgccg
     1201 agcc
//
LOCUS       DASUCJ010001239         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1052546, whole genome shotgun sequence.
ACCESSION   DASUCJ010001239 DASUCJ010000000
VERSION     DASUCJ010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052546"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VFU90_14475"
     CDS             complement(<1..1105)
                     /locus_tag="VFU90_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481048.1"
                     /translation="MTARRRTQRPRFAALVPIAFAAACSSGGGGSAAPPVAGPTAYPS
                     APPATTPIAHVVVMVQENRSFDNLFATYPGADGSTHGTLHNGKTFQLKKGRLAGKELD
                     HMHSGFVTEYDDGKMDGFDKIGFGSSGTGGPAGTYPLRYVNPSQIAPYWFLAQHYALA
                     DHLFQTQSSGSFTAHEDLIAGGTALNATESIIDFPSRGPWGCDAPAGTKTSLITDKGD
                     YLFNKGPFPCLKYATIADRLDPDGLSWKYYTPSMQPGSSGFLWNAFDAISAIRYGPDW
                     KSHVIIPETTILKDVAANRLPSVAWVIPSGQNSDHAASLDTGPQWVASVVDKIGESPY
                     WNSTAIIVVWDDWGGYFDHVAPAQLDYQGLGFRV"
     gene            complement(1114..2484)
                     /locus_tag="VFU90_14480"
     CDS             complement(1114..2484)
                     /locus_tag="VFU90_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481049.1"
                     /translation="MGSARTFVSVFAVALAAAACGGSGASLTPGGRGGSPNALSTKYV
                     KHIVLMIQENRSFDDLFATFPGADGATFGYNSKGKQIRLKKSSLAAADINHDWSTFLA
                     ECDPQGSTCKMDGFDKAKIGGNNPGGNYAYQYVDPNQIKPYWAIAQQYALLDHMFQTQ
                     GSGSFTAHQDLIAGATALDDTYSFIDYPSEWKVWGCDAPPGTTVPLISVDDAYKGSGK
                     LPPPCVKNYPGGTLRDLLDAKHVPWKYYTPPYNAKKYGAGNEWNAFAVIKAVRYGPEW
                     TTNVSIPESNICSDISNRTLPAFSWVIPDQVDSDHPQSKNQQDDGPDWIASVIDKIGK
                     SPYWSSTAIIVVWDDWGGFYDNVAPKFYGSGQLGFRVPALVVSPYVGQGKISHDEFEF
                     GSILKFAEQTFELGSLGTTDVRAKSIGSIFEFNKPARAFKPIPTSRSCDYFMHRPPSY
                     LPVDTE"
     gene            2613..3509
                     /locus_tag="VFU90_14485"
     CDS             2613..3509
                     /locus_tag="VFU90_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5481050.1"
                     /translation="MMTVVDYLLLDVFTDRAFAGNPVAIFPDVSLDDATMQAVARELN
                     LSETVFLTRVTDGAIAALRIFTPSREVLFAGHPTVGTAIALAGRLHWPGSDAPKFALR
                     LRIGDVPIAIDRDGVMRAWLTTPPVTFSREIAREDAAAMLSLGVADVRADLPPQLLGA
                     GNPFLYVPLVGNAAVDRAMLDVSAVRAHVGWDDVNGVYVFAQNDDGAYARLLAPMAGI
                     AEDPATGSATGPLYAYLARCGALPRKDRFVNLQGVAMGRPSTLHVRLRWDGEVPNAIE
                     VGGGAVFVGEGRLHVGASGAGG"
     gene            3506..>4158
                     /locus_tag="VFU90_14490"
     CDS             3506..>4158
                     /locus_tag="VFU90_14490"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5481051.1"
                     /translation="MTLLPATPENLERAAACLRAGGVVAFPTETVYGLAANAFDARAV
                     ARVFEIKARPEFDPLIVHVAGEAMVARVAAEISPRARRLMQRFWPGPLTIVVPKQTAL
                     PELVTSGLPTVAVRMPAHPAARELIERAGVPLAAPSANPFGRLSPTRAAHVERMLGDR
                     VDVIIDGGPTAHGLESTIVELEPVPAILRPGAISREELEDALGEPVSALPPAEARPRA
                     "
BASE COUNT          678 a         1304 c         1404 g          772 t
ORIGIN      
        1 gaacgcgaaa cccgaggccc tgatagtcga gctgcgcggg cgcgacgtga tcgaaatagc
       61 cgccccagtc gtcccatacg acgatgatgg cggtggaatt ccagtacggg ctctcgccga
      121 tcttgtcgac gacggatgcg acccactgcg gcccggtatc gagcgaggcg gcgtgatcgg
      181 agttctgtcc gctcgggata acccaggcca ccgagggaag ccgattggcg gcgacgtcct
      241 ttaagatcgt cgtctcggga atgatgacgt gagacttcca gtcgggaccg taacggatcg
      301 ccgaaatcgc atcgaaggcg ttccacagga atccgctgct gcccggctgc atcgaaggcg
      361 tgtaatactt ccaggacagg ccgtcggggt cgagacggtc ggcgatggtc gcgtatttca
      421 gacacgggaa cggtcccttg ttgaacaagt aatcgccttt gtccgtgatg agcgaggttt
      481 tcgtgccggc cggcgcgtcg catccccacg gtccgcgcga cgggaagtcg atgatgctct
      541 cggtggcgtt gagtgcggtc ccccccgcga ttaaatcctc atgggcggtg aagctgccgc
      601 tgctttgcgt ctggaagaga tggtcggcga gcgcgtagtg ctgcgccagg aaccagtagg
      661 gtgcgatctg cgacggattg acgtaccgca gcggatacgt tccggcgggt ccgccggtac
      721 cgctcgaacc gaagccgatt ttatcgaatc cgtccatctt tccgtcgtcg tactcggtga
      781 cgaacccgga gtgcatgtgg tcgagctctt tgccggccag ccgccccttc ttcagctgga
      841 acgtcttgcc gttgtgcagc gtaccgtgcg tcgagccgtc ggctcccgga taggtcgcga
      901 acagattgtc gaagctgcgg ttttcctgaa ccatcacgac gacgtgcgcg atcggggtgg
      961 tggccggcgg tgccgaggga taggcggtcg gcccggcaac cggcggtgcg gcggagccgc
     1021 cgcctcccga cgaacaggct gccgcgaacg cgatcggcac gagggcggca aatcttgggc
     1081 gttgggtccg gcggcgtgca gtcatcgcgt cctctattcg gtatcgaccg gcaagtacga
     1141 cggcggccgg tgcatgaagt aatcgcacga gcgggaggtc ggaatcggct tgaaggcccg
     1201 agcgggcttg ttgaactcga agatgctgcc gatcgacttc gcgcgcacgt cggtcgtgcc
     1261 gagcgatccc agctcgaagg tctgctcggc gaacttcaag atgcttccga actcgaactc
     1321 gtcgtgcgag atctttccct gtccgacgta cggcgagacg accaaggccg gtacgcgaaa
     1381 gccgagctgc ccggagccgt agaacttcgg tgcgacgttg tcgtagaagc cgccccaatc
     1441 gtcccacacc acgatgatgg cggtcgagct ccagtacgga ctctttccga tcttatcgat
     1501 caccgaagcg atccagtcgg gaccgtcgtc ctgctggttt ttgctttgcg ggtgatccga
     1561 atcgacctga tcgggaatca cccacgagaa tgcgggcagc gtccggttgc tgatgtcgct
     1621 gcagatgttg ctctcgggaa tcgaaacgtt ggtcgtccac tccggaccgt accgcaccgc
     1681 cttgatcacc gcgaaagcgt tccactcgtt gccggcgccg tacttctttg cattgtaggg
     1741 gggcgtgtag tatttccagg gcacgtgctt ggcgtcgagc aggtcgcgca gcgtgccgcc
     1801 ggggtagttc ttcacgcacg gcggcgggag ctttccgctg cctttatacg cgtcgtcgac
     1861 cgaaatcagc ggaacggtcg ttcccggagg agcgtcgcag ccccagacct tccactcgga
     1921 cggatagtcg atgaagctgt acgtgtcgtc gagggcggtc gcgccggcga tcagatcctg
     1981 atgggcggtg aagctgccgc tgccctgcgt ttgaaacatg tgatcgagca gcgcgtactg
     2041 ctgggcaatc gcccagtacg gtttgatctg gttcgggtcg acgtactggt acgcgtaatt
     2101 cccaccggga ttgttgccgc cgatcttggc tttgtcgaag ccgtccattt tgcaggtgct
     2161 gccctgcggg tcgcactccg cgagaaacgt gctccagtcg tgattgatgt cggcggccgc
     2221 gagcgagctc ttcttgagcc tgatttgctt gcctttcgag ttgtaaccga acgtcgcgcc
     2281 gtccgctccg gggaaggtcg cgaacaagtc gtcgaagctg cggttttcct ggatcatcaa
     2341 gacgatgtgt ttgacgtact tcgtgctcaa ggcgttcggc gatccgccgc ggccgccggg
     2401 cgtcagcgac gcaccgctgc cgccgcacgc cgcggccgcg agcgcaacgg caaacaccga
     2461 tacgaaggta cgcgcagagc ccaccagccg ttcctttcga aagcccgcgc cggagagcgg
     2521 cgcatcgcag gcttgggtct atcccgcatc cgggcaatct cctcccgttg ttatagaagg
     2581 aagcgagcga tgagagcgcc attcaacgtt tcatgatgac cgtagtcgac tatctgttgc
     2641 tggacgtctt caccgaccgg gcgtttgcag ggaatccggt cgcaatcttt cccgacgttt
     2701 cgctcgacga cgcgacgatg caagcggtcg cgcgcgaact caatctctcc gaaacggtgt
     2761 ttctcacgcg cgttacggac ggtgcgatcg ccgcgctgcg catctttacg cctagccggg
     2821 aagtgctgtt cgccgggcat ccgaccgtcg gcaccgccat cgcattggcc ggccggctgc
     2881 actggccggg aagcgacgcg ccgaagttcg cactgcgctt gcggatcggc gacgtaccga
     2941 ttgcgatcga tcgcgacggc gtgatgaggg cctggctgac gacgccgccg gtcaccttca
     3001 gccgcgagat cgctcgcgag gacgccgccg cgatgctatc gctcggcgta gccgacgtgc
     3061 gcgccgatct gccgccgcag ctgctgggtg cgggcaaccc gttcctgtac gttccgctcg
     3121 tcggcaatgc agccgtcgac cgcgcgatgc tggacgtttc cgccgtgcgg gcgcacgtgg
     3181 gatgggacga cgtcaacggc gtgtacgtgt tcgctcaaaa cgacgacggc gcgtacgccc
     3241 gtctgctggc accgatggcc ggcatcgcgg aagatccggc aaccggaagt gccaccgggc
     3301 cgctgtacgc atatctcgcg cgctgcggcg cgctgccgcg caaggatcgc ttcgtgaacc
     3361 tgcagggcgt cgcgatggga cgcccctcga cgctgcacgt gcggctgcgc tgggacggtg
     3421 aggtaccgaa cgcgatcgaa gtcggcggcg gcgcggtgtt cgtgggcgaa gggcggctgc
     3481 acgtcggagc atccggcgcg ggaggttgac gctgcttccg gcgacacccg aaaacctcga
     3541 acgggcggcc gcatgtctgc gcgcgggcgg cgtggtcgcc tttccgaccg agaccgtcta
     3601 cgggctcgcc gccaacgcgt tcgacgcgcg cgcggtcgcg cgcgtcttcg aaatcaaagc
     3661 gcgcccggaa ttcgatccgc tgatcgtgca cgtcgccggc gaagcgatgg tcgctcgcgt
     3721 cgccgccgag atctcaccgc gagcccgtcg cctgatgcaa cgcttctggc ccggtccgct
     3781 cacgatcgtc gttccgaagc aaacggcgtt gccggagctc gtgacgtcgg gtttgccgac
     3841 ggtggcggtg cgaatgccgg cccatccggc ggcgcgcgaa ctcatcgagc gggccggcgt
     3901 gccgctggcg gcgccgagtg cgaatccgtt cggacgattg agcccgaccc gcgccgcgca
     3961 cgtggaacgg atgctgggcg accgcgtgga cgtcatcatc gacggcggtc cgaccgcaca
     4021 cggtctcgag tcgacgatcg tcgagctcga accggtgccc gcgatcctgc ggccgggggc
     4081 gatttcccgg gaagagctgg aagacgcact tggcgagccg gtttcggcgc tgccgcccgc
     4141 cgaagcgcgg ccgcgggc
//
LOCUS       DASUCJ010001240         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1021854, whole genome shotgun sequence.
ACCESSION   DASUCJ010001240 DASUCJ010000000
VERSION     DASUCJ010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021854"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFU90_14495"
     CDS             <1..248
                     /locus_tag="VFU90_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5481052.1"
                     /translation="WIVLGLIAGFVARWIVPGEGPGGIVGDIVVGILGAIIGGWIYGL
                     FSHVGVTGFNLPSIVCAIIGAVVLLFIIRAIRGRPAT"
     gene            300..542
                     /locus_tag="VFU90_14500"
     CDS             300..542
                     /locus_tag="VFU90_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU5481053.1"
                     /translation="MAYVITEPCIGTKDKSCVDVCPVDCIHGKDEDEMLFIDPEVCID
                     CGACVSACPVEAIFADSDVPEQYEKYTEINAAYFKK"
     gene            complement(555..1406)
                     /locus_tag="VFU90_14505"
     CDS             complement(555..1406)
                     /locus_tag="VFU90_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5481054.1"
                     /translation="MNDATTRLREAMVAQLERAGVLRTPAVAAAMRAVPRHLFLPGLA
                     LDDAYADRAVALKTDGSQIVSSVSQPAMIALMLELLAASPGDRLLEIGTGSGYNAALL
                     AEIVGPSGSVTTLDLDAELAERAAAVLRELGYATVRVTAADGGAAVSESGAFDGVIVT
                     ARSDDVASAWWRALARRARLVLPLRLEGAGEYAVGFSLECDGRLHSVGVHPCAFIALR
                     GEAAVESTSTIFYRHPETRGSSPACLRAVAEITAVRRENATPALLGDADVVIARPVSV
                     FAVTFAA"
     gene            1469..1717
                     /locus_tag="VFU90_14510"
     CDS             1469..1717
                     /locus_tag="VFU90_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481055.1"
                     /translation="MDTQISPSAFDHFFAWAPLTIGVLIYATFYVAKRHEATEPGVPI
                     GQTFACANCGKRGKREHMVPREHAGAMSWYCARCAGGH"
BASE COUNT          299 a          586 c          603 g          292 t
ORIGIN      
        1 cgtggatcgt cctcggcctg atcgccggct tcgtcgcgcg ctggatcgta cccggcgaag
       61 gccccggcgg gatcgtcggc gacatcgtgg tcggcatcct gggggcgatc atcggcggct
      121 ggatttacgg gctgttcagc cacgtgggcg tcaccggttt caacctgccg agtattgtct
      181 gtgccatcat cggcgcggtc gtgctactct ttattatacg cgcgattcga ggccggccgg
      241 caacgtaacg cgccgccgcc agtacttcgt catcgctctt ccgtaagggg ttaccgttca
      301 tggcctacgt catcaccgaa ccgtgcatcg gcacgaagga caagtcgtgc gtggacgtct
      361 gtccggtcga ctgcattcac ggcaaagacg aggacgagat gctcttcatc gaccccgaag
      421 tgtgcatcga ttgcggcgcc tgcgtttcgg cctgtccggt cgaagcgatc tttgccgact
      481 ccgacgtgcc ggagcagtac gagaaataca ccgagatcaa cgcggcctac ttcaagaagt
      541 aacgcggcgc cgcgtcaggc ggcgaacgtg acggcgaaca cgctcaccgg acgggctatc
      601 acgacgtcgg catctcctag gagagccggc gttgcgtttt cgcggcgcac ggcggtaatc
      661 tccgcaaccg cgcgcaggca ggcgggactg ctgccgcgag tctccggatg acggtagaaa
      721 atggtgctcg tgctctcgac ggcggcttcg ccgcgcagcg cgatgaaggc gcacggatgg
      781 acgccgacac tgtgcagccg cccgtcgcac tcgagtgaga aaccgacggc gtactcgccg
      841 gcgccctcca gccgcagcgg gagtaccaat cgcgcgcgcc gcgccagcgc gcgccaccac
      901 gccgaggcga cgtcatcgct gcgcgccgtg acgatcacgc cgtcgaacgc accgctttcc
      961 gaaaccgcgg cgccgccgtc ggcggcggtt acgcggacgg tcgcgtaccc aagctcccgc
     1021 aagaccgcgg cggcgcgttc ggccagctcg gcgtcgagat cgagcgtcgt cacgctgccg
     1081 ctcggcccca cgatctcggc caatagggcg gcgttgtagc cgctgccggt tccgatttcc
     1141 aggagacggt cgccgggcga cgcggcgagc agctcgagca tcagcgcgat catcgccggc
     1201 tgcgagaccg acgagacgat ttggctgccg tcggttttga gggcgaccgc ccgatcggcg
     1261 taggcgtcgt cgagggcgag cccgggaagg aacaggtggc gcgggacggc gcgcattgcg
     1321 gccgccaccg caggcgtccg caggacgccc gcgcgttcga gttgcgcgac catcgcctcg
     1381 cggagtcgcg tggtggcgtc gttcacggtc cgggcttttt cgacccgaag cccgaggtgc
     1441 gcttctaacc gggcgggaaa aagcgggcat ggatacgcaa atctcgccct cggcattcga
     1501 ccatttcttc gcgtgggcgc ccttgacgat cggggtgctg atctacgcga cgttctacgt
     1561 ggcgaagcgg cacgaggcga ccgagccggg cgtgccgatc ggtcagacct ttgcgtgcgc
     1621 caactgcgga aaacggggaa aacgcgaaca catggtgccg cgcgaacacg ccggcgcgat
     1681 gagctggtac tgtgcccgct gcgccggcgg tcactaaccg gtacgcccgt tcccgcacgt
     1741 cagcagagct gacgcgttat gcgaaacgct agcaaaattc
//
LOCUS       DASUCJ010001241         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_273012, whole genome shotgun sequence.
ACCESSION   DASUCJ010001241 DASUCJ010000000
VERSION     DASUCJ010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273012"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFU90_14515"
     CDS             complement(<1..465)
                     /locus_tag="VFU90_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481056.1"
                     /translation="MARIALDAMGGDRAPEVPIAGALLALAELDGVHSIQLVGRADDV
                     AAELDRQLGAAASLPSGLRDRITIVDAADVIEMSEKPTAAIRSKPNSSMAVGLKLHAT
                     GNADAFVSAGNTGAQMAASVFLLKLHAGLTRPAIATTFPTQRNPVVVLDAGAN"
     gene            complement(468..650)
                     /gene="rpmF"
                     /locus_tag="VFU90_14520"
     CDS             complement(468..650)
                     /gene="rpmF"
                     /locus_tag="VFU90_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5481057.1"
                     /translation="MAVPKRRTSKRKKRARNTHKAAAPIAIQACPRCQSMKRPHHVCP
                     ECGYYAGEQRVEAREA"
     gene            complement(682..1191)
                     /locus_tag="VFU90_14525"
     CDS             complement(682..1191)
                     /locus_tag="VFU90_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5481058.1"
                     /translation="MLCYDIREVERQAVRVDGDLELDDPIWTEGDVRPATPVHATGRL
                     SRAGTGRYYWSGRIEAKAVTACRRCLKDVSSSVSEEVHVLFVEPGDEVADDPDTYRIP
                     PHAHNIDLRPAVREQWMLAAPEYVLCRDDCRGLCARCGADLNAAPCSCPPETDSRWTA
                     LAAARRVSH"
     gene            complement(1262..1681)
                     /locus_tag="VFU90_14530"
     CDS             complement(1262..1681)
                     /locus_tag="VFU90_14530"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5481059.1"
                     /translation="MAGTRTLTIIKPDAFGAGKTGKIIAHLEGAGFKIRAARVLRLTQ
                     PQAEAFYEVHRARPFFASLTRFMTSGVCMPMLLERADAVSTLRSVIGATDPAEAAAGT
                     VRKLYAESKERNAIHVSHSDENADREAAFFFAEADRI"
BASE COUNT          308 a          575 c          633 g          312 t
ORIGIN      
        1 gttggcgccg gcgtcgagga cgacgacggg attgcgctgg gtgggaaagg tagtggcgat
       61 ggccggtctc gttaggcctg cgtggagctt gagcagaaac acggacgcgg ccatctgcgc
      121 gccggtgttg ccggcggaga cgaaggcgtc cgcgttgccc gtcgcgtgca gcttgaggcc
      181 aacggccatc gagctattgg gcttggaacg gatggccgcg gtcggctttt ccgacatctc
      241 gatcacgtcg gccgcatcga cgatcgtgat gcgatcgcga agtcccgacg gcagcgacgc
      301 ggcggcgccg agttgtctgt ccaactccgc cgcgacatca tcggcgcggc cgacgagttg
      361 gatcgagtgt accccgtcga gctcggccag tgcgagcagg gcgccggcga tcggcacttc
      421 gggggcgcga tcgccgccca tcgcatccaa cgcgatgcgt gccaacgcta tgcttccctg
      481 gcttcgaccc gctgctcgcc ggcgtagtag ccgcactcag gacagacgtg gtgcggacgc
      541 ttcatgctct ggcagcgcgg gcacgcctgg atcgcgatcg gcgccgctgc tttgtgcgtg
      601 ttacgcgcgc gctttttccg cttcgatgtt cggcgcttgg ggacggccat gggagatcaa
      661 ccgaaagtgt gagagatgag ctcagtgcga gactcggcgg gccgcagcga gcgcggtcca
      721 gcgtgagtcg gtttccggcg gacacgagca cggtgccgcg ttgagatcgg ctccgcaccg
      781 ggcgcacagg ccgcggcaat cgtcgcggca gaggacgtac tccggcgcgg cgagcatcca
      841 ttgctcgcga accgctggac gaagatcgat gttgtgggca tgcggcggaa tacgatacgt
      901 gtctggatcg tcggccactt catcgcctgg ttcgacgaac aggacgtgga cctcttctga
      961 cacggagctg gagacatcct tgagacagcg ccggcaggct gtgactgcct tggcttcgat
     1021 gcgaccgctc cagtagtagc gcccggttcc agcacgggac aaacggccgg tggcgtgaac
     1081 tggcgtagcg ggtcgcacgt cgccctcggt ccagatcggg tcgtccaact ccaggtcccc
     1141 gtcgacgcgg accgcctgcc gctccacctc gcgaatgtcg tagcacagca taacccatga
     1201 atgtacttag agatgagacc cggagcaagg cggccgccgc gcgacgaatg gcgcaaagcg
     1261 gctagatccg atcggcctcg gcgaagaaga acgcggcctc gcggtcggcg ttctcgtcgg
     1321 aatgcgagac gtgaatggcg ttccgctcct tggattccgc gtacagcttg cgcaccgtgc
     1381 ccgccgccgc ctccgcggga tcggtggcgc cgatcaccga gcgcagcgtc gaaacggcat
     1441 ccgctcgctc gagcagcatc ggcatgcaca cgccggacgt catgaagcgc gtgagcgacg
     1501 cgaagaaggg gcgcgcgcga tgcacctcgt aaaacgcctc ggcctgcggc tgcgtgagcc
     1561 ggagaacccg cgccgcacga atcttgaatc ctgcgccctc cagatgcgcg atgatttttc
     1621 cggtttttcc cgcgccaaag gcgtcgggct tgatgatggt cagcgtccgc gtgccagcca
     1681 tggcgtcacg atcctatgag tttgcgaacg atgtcgccgc gcgtgaggaa gccgaccagg
     1741 cggccgtccc gcacgacggg gacgcgttcg acgtctttgt tggacatcac ggaagcgact
     1801 tcggctaacg gttgatcggg cgacacgc
//
LOCUS       DASUCJ010001242         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1373339, whole genome shotgun sequence.
ACCESSION   DASUCJ010001242 DASUCJ010000000
VERSION     DASUCJ010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373339"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..543
                     /locus_tag="VFU90_14535"
     CDS             172..543
                     /locus_tag="VFU90_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5481060.1"
                     /translation="MHVDGVTRLIAVHAHATPAAPANLAFDDAQTAHSARGVRRLVAL
                     VTDAYGNPVSGVPVRFATQSGSVSPSRAVTDANGRVALAWTVGSRTGELTVSGIANGR
                     DVKGTYTARVASIHAAPHTRR"
     gene            652..>1085
                     /locus_tag="VFU90_14540"
     CDS             652..>1085
                     /locus_tag="VFU90_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481061.1"
                     /translation="MLRPLGVFAAGMVALAVVVSASPGQSPSATPLGYYRYPAIHDTT
                     IVFTAEGDLWTIGLGGGVARRLTSGRGEEGDASISPDGATIAFTGQYEGPAEVYTMPI
                     AGGLPERRTWDGARDVVVGWTPDGRVLYSTRQFATLPIDQLVT"
BASE COUNT          158 a          380 c          372 g          175 t
ORIGIN      
        1 tcgatttccg tacgcgtggt ggacgcgcac ggcaatcagg tgcgcggcgt gcaggtggtg
       61 ctgtcgccgt ccggcggcac cgtgcccgat acggcgctcg ggaccgattc gtgagtcgtc
      121 gcccggacgc gctggacgct gggccgcacc gccggccacc taacgcgggc ggtgcacgtg
      181 gacggtgtca ccaggctgat cgcggtgcat gcgcacgcga cgccggccgc gcccgcgaac
      241 ctcgcgttcg acgacgcaca gaccgcgcac agcgcccggg gcgttcggcg cctcgtcgcg
      301 ctggtgaccg atgcctacgg caatcccgtc tccggcgtgc cggtgcgatt cgcgacacag
      361 agcggatcgg tctcgccgtc gcgcgcggtg accgacgcga atgggcgtgt ggcgctcgca
      421 tggaccgtgg gcagccgcac cggcgaattg accgtgtcgg gaatcgcgaa cggacgcgac
      481 gtgaaaggca cgtacacggc acgcgtcgcc tcgatccacg cagcaccgca cacccggcgc
      541 taatcgctcg cgcgttcgcc ccgttgggct tgctacatca agttccgtgc tgtaaggtcg
      601 tgccggtcga tcccacctgt tggatctccg tccatctccc tcggcacacg tatgctcaga
      661 cccctcggtg tgttcgcggc cggcatggtc gctctggcag tcgtcgtgag cgcttcgccc
      721 ggccaatcgc cgtccgcgac accactcggc tactaccggt atccagccat ccacgacacg
      781 accatcgtgt tcaccgccga gggcgatttg tggacgatcg ggttgggcgg cggcgtcgcg
      841 cgacgtctca cgagtggccg cggcgaagag ggcgacgcgt ccatttcgcc ggacggcgcg
      901 acgatcgcgt tcaccggaca atacgaaggg cccgccgaag tctacacgat gccgattgcc
      961 ggcggccttc ccgagcggcg cacctgggac ggcgcacgcg atgtcgtggt gggatggacg
     1021 cccgacggcc gcgtgctcta ttcgacgcgg cagtttgcga cgctgcccat cgaccagctc
     1081 gtgac
//
LOCUS       DASUCJ010001243         1789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_28501, whole genome shotgun sequence.
ACCESSION   DASUCJ010001243 DASUCJ010000000
VERSION     DASUCJ010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28501"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFU90_14545"
     CDS             <1..522
                     /locus_tag="VFU90_14545"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEU5481062.1"
                     /translation="SWAEGVYKAATMGSETTAAAAGKVGEVRRDPFAMLPFCGYHIGD
                     YFEHWLRIGSAVGAPPEIFSVNWFRKDAEGRFVWPGFGQNMRVLKWIVERCAGRAEAV
                     ETALGLVPRYDDLDWTGIEFAVDRFQEVMRIDRAQWEREIALHDALFERLGERRPEAL
                     MGERERLFRELSM"
     gene            complement(775..939)
                     /locus_tag="VFU90_14550"
     CDS             complement(775..939)
                     /locus_tag="VFU90_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481063.1"
                     /translation="MPILFGVSNGHLRDHYGETPQSLDVALASVGVKSISAPLRAPVG
                     CNRMIPWSRR"
BASE COUNT          284 a          566 c          597 g          342 t
ORIGIN      
        1 agctgggccg agggtgtcta caaggccgct acgatgggtt ccgagacgac ggccgcggcc
       61 gccggcaagg tgggcgaagt ccggcgcgat ccgttcgcaa tgctgccatt ctgcgggtac
      121 cacatcggcg attacttcga gcactggctt cgcatcggca gcgcggtcgg cgcgccgccg
      181 gagatcttca gcgtgaactg gttccgcaag gacgccgaag gccgcttcgt ctggccgggt
      241 ttcgggcaga acatgcgcgt gctgaagtgg atcgtcgagc gctgcgccgg gcgcgccgaa
      301 gctgtcgaga ccgcgctcgg cctcgtgccg cgctacgacg atcttgactg gaccgggatc
      361 gaattcgccg tcgaccgctt ccaggaagtg atgcgcatcg accgcgcgca gtgggagcgc
      421 gagatcgcgc tgcacgacgc gttgttcgaa aggctcggcg aacggcgtcc cgaggcgctg
      481 atgggcgagc gcgagagact gttccgggaa ttgtcgatgt aagcgcttac tctcttgtgt
      541 attgatcgag cgggagcgct cgacaaatca tcccaatgtg ttgttgcaat tgaggattat
      601 tgtccgcgtc agggtccgga tctgcgcttc ccccacgcag ctgcggcgga cgcagctcaa
      661 gcttggacgc tgccgcggcg cgtaactggc accctagttc ctcacggttg gggccgctcg
      721 ggaccggcga ggctcccggc ggttcaatta gcttgcacgc ggcgaggtcg tcgatcatcg
      781 tcgagaccac ggaatcatcc ggttgcaccc gaccggggcg cgcagcggtg cggagatgga
      841 cttgacgccg acgcttgcca gcgcgacgtc gaggctctgt ggcgtctcgc cgtagtggtc
      901 gcgtaagtgg ccgttcgaaa caccgaagag tatcggcacc cggccattcg gtagtgtctc
      961 aggctgcccg cgcggcgctc gccgtgtacc gcgacagcct ccgagtcctc ggtgtcgcgc
     1021 tcgatgacgc tgggccgaac gcccgtgcgc gccaaccgtt cgtcgctcgg gacgcgttcg
     1081 acgtcgatca tgctgcccat gtagcattcg gggataactt ggccatagga atcgtgcgcg
     1141 tctcgtcgcg ccaatgccga cagggcgcgg catctgaccc gacccggacc tcgcgtttcg
     1201 agaccttcgc cgcacttctg caggaggccg agttcttcgc gcggtccacg acgggggtgg
     1261 cgctgtttca cgtgaaacac gaggccacga agtttcaggt cggctgcacg gcgcgccgcg
     1321 cccggccgca cgctaacgtc gccgcgccta ttctcgccgt agcccggcac agtgggcctg
     1381 gatgcggcgt gaccacgacc actccccgga ttcgtgtgct tcgctacggc atgagatgcc
     1441 ggcgatatga ggtggcggtg atcttcgcga gatcgtcgcg gctctcttga aggtgcgcac
     1501 gtgtatcggc gctcgctgat cgcgtccgat cccgcttcgg ggtgcttcag gggcatggtt
     1561 ccacgtgaaa catgaagcac gcggtccgcg gcgactactc ggccaccgct gaccccgatc
     1621 ggcacctgtt ggtttcgatc gacgcttcag ccttgctgcc gatcgattca cggcgccgga
     1681 tacgctacaa tcgccgctct gcccgcattg ggtcgatctc tccaagctac tgattccaat
     1741 gaaatttcat aagcgattcg atgtgatcgt cgtaggcggc gggcacgcc
//
LOCUS       DASUCJ010001244         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_212212, whole genome shotgun sequence.
ACCESSION   DASUCJ010001244 DASUCJ010000000
VERSION     DASUCJ010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212212"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="cgtA"
                     /locus_tag="VFU90_14555"
     CDS             <1..847
                     /gene="cgtA"
                     /locus_tag="VFU90_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEU5481064.1"
                     /translation="PRFAERGEPGERCELRLELRLLADCGVVGVPNAGKSTLLSVVSA
                     ARPKIADYPFTTIEPQLGVVRVSDDESFVMVDVPGLIEGAHAGAGLGDRFLRHVERTR
                     VLVHLLDGAKPLEGILDDKTTIERELGSWNPRLLEKPALLVLNKLDLPQARERLAELR
                     ERFPDVRGISAATREGVRDLIYAVYRTIERTPPPEVVTPPPATIELTPAGAFELKRAR
                     DGAFELSGERIERLAAMTDFDSDESLVRFERALGRMGVDKKLREMGAVEGDTVRIGEY
                     EFTYS"
     gene            844..966
                     /locus_tag="VFU90_14560"
     CDS             844..966
                     /locus_tag="VFU90_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5481065.1"
                     /translation="MKLGIFGGTFDPIHNAHLYIAESARVVEKLDKVLFVPANK"
BASE COUNT          175 a          331 c          331 g          168 t
ORIGIN      
        1 gccgcggttt gccgagcgcg gcgaacccgg cgagcgctgc gaactccgtc tcgaactgcg
       61 cttgctggcc gactgcggcg tcgtcggcgt tccgaacgcg gggaaatcga cgctgctctc
      121 cgtcgtttcg gccgcgcgcc caaagattgc cgattatccg ttcacgacga tcgaacccca
      181 gctcggcgtg gtccgcgtct ccgacgacga atcgttcgtg atggtcgacg tccccggctt
      241 gatcgaaggc gcgcacgccg gcgccggact cggcgaccgc ttcttgcgcc acgtcgagcg
      301 gacgcgcgtg ctcgtgcatc tgctcgacgg cgctaagccg ctcgagggaa tcctggacga
      361 caagacgacg atcgaacgcg aacttggctc gtggaacccg cgactgctcg agaagccggc
      421 gttgctggtg ctcaacaaac tcgatcttcc gcaagcgcgc gagcgcctgg ccgaactgcg
      481 cgaacgcttt ccggacgtgc gcggcatcag cgcggcgacc cgagagggcg tccgcgacct
      541 gatctacgcc gtctatcgaa cgatcgaacg cacgccgccg cccgaggtcg tcaccccgcc
      601 gccggcgacg atcgagctga cgccggccgg cgcgttcgag ctgaagcgcg cgcgcgatgg
      661 agcgttcgag ctttcgggcg agcgcatcga gcggctcgcg gcgatgacgg acttcgattc
      721 cgacgaatcg ctggtgcgct tcgagcgcgc gctgggccga atgggcgtcg acaaaaaatt
      781 acgcgaaatg ggcgccgtcg aaggggatac ggtgcgtatc ggagagtacg agttcaccta
      841 ttcgtgaagc tcggcatttt cggcgggacg ttcgacccta ttcacaacgc gcatctttac
      901 atcgcggagt cggcgcgcgt ggtggagaag ctcgataagg tcctcttcgt tccggccaac
      961 aagtagcact accgcgacac cgcgcacgcg tcgatcgaac atcgc
//
LOCUS       DASUCJ010001245         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_869118, whole genome shotgun sequence.
ACCESSION   DASUCJ010001245 DASUCJ010000000
VERSION     DASUCJ010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869118"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFU90_14565"
     CDS             complement(<1..566)
                     /locus_tag="VFU90_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5481066.1"
                     /translation="MLEADNLAAIDVPRDELVRYRRYFHAHPELSMEEVETAAFIERE
                     LRSCALDEIRTGIGKTGILATLCGGRPGPVTLLRADMDALPIRELTECEYASTREGVM
                     HACGHDGHMAILLSAARFLSARRADVPGTLVFCFQPGEEGAAGNRLMIEDGALENPHV
                     DRTFALHLYSGLDAGSVGIRDGPFFASS"
     gene            651..1652
                     /locus_tag="VFU90_14570"
     CDS             651..1652
                     /locus_tag="VFU90_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5481067.1"
                     /translation="MDTIDQGLSHEQLVTMFRTMLAQRMLENRGFQLNRQGKIPFASA
                     SEGHEAVQAGAAMAFVRGQDILVPYYRDLGLNLGIGLTPYEVLLSLFARAADHSAGRQ
                     FPHHYASRRLGLQTISSVIAAQLPHAVGAAYALAYRGERGRAVLTTFGDGATSEGEWH
                     ESLNFAAVHSLPIVFLCENNEWAISTPLSKQTAEPDIYKRAEGYGMPGVRVDGMDPIA
                     CYAAVKLAMDRARSGAGPTLVEAKCYRFLSHTTDDDDRTYRSRTEVEERRRNDPVPRF
                     EALLIERGILTPEAAESLKRSVLEEANEATDRAETMPYVDRQQLFEEVYADGWQPWR"
     gene            1695..2723
                     /locus_tag="VFU90_14575"
     CDS             1695..2723
                     /locus_tag="VFU90_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5481068.1"
                     /translation="MAKTNTQAQTLVERYGLTDEQLRQIFRNMLLQRQLDNRGFQLNR
                     QGKVPFALGSEGHEALQAGAAMAFERGKDILAPYYRDLGLSLGIGLTPFEILLSIFAR
                     AADHNGGRQFPNHYSSKAAGLMSFSSILAAHLPHAVGAAYALAYRGETGRAVLATCGD
                     GTTSEGEWHESMNFAAIHKLPFVMLVENNEWAISTPLSKQMAQPEIYKRAAGYGMPGR
                     RFNGFDPVETYGAVKEAMERARSGGGPSLIEGTCYRYLAHSTDDNDMTYRTREDVLEH
                     RKLDPVPRFEAVLVENGIMTEPEIEAMRKDVLRETNEATDAAEAMPYPQPSDLYTHVY
                     EGAWQPWQ"
BASE COUNT          535 a          964 c          919 g          454 t
ORIGIN      
        1 tcggacgacg caaagaacgg gccgtcgcga atgccgacgc tgcccgcgtc cagaccgcta
       61 tagagatgga gggcgaaggt ccggtcgacg tggggattct cgagcgcccc gtcctcgatc
      121 atcagccgat tccccgccgc gccctcctcg ccgggctgaa aacagaagac cagcgtaccc
      181 ggcacgtccg cccggcgcgc gctcaagaac cgcgccgccg acagcaggat ggccatgtga
      241 ccgtcgtgac cgcaggcgtg catgacgccc tcgcgggtgg acgcgtactc gcactccgtc
      301 agctcccgga tcggcagcgc gtccatatcg gcccgcagca gcgttaccgg cccggggcgc
      361 ccaccgcata acgtggccaa gatgccggtc ttcccgatcc cggtgcgaat ttcgtcgagc
      421 gcgcacgagc gcagctcgcg ctcgatgaac gcggcggtct cgacctcttc catcgacagt
      481 tccgggtgcg cgtggaagta ccggcgatag cgaaccagct cgtcgcgcgg tacgtcgatc
      541 gccgcgaggt tatcggcctc caacatgcgt gcggcaagag tttcgggcgc gcgccgcccg
      601 gtgcctggcg caggatttcc ccgcgaaggg gggcgagtac cgatactcct gtggacacga
      661 tcgaccaggg gctttcccac gagcagctcg tgacgatgtt ccgtacgatg ctcgcgcagc
      721 ggatgctcga gaaccgcggc tttcagctca accggcaggg caagattccg ttcgcctcgg
      781 ccagcgaagg gcacgaggcg gtgcaggccg gcgcggcgat ggccttcgtc cgcggccagg
      841 acatcctagt gccgtactat cgcgacctcg gcctcaacct cggaatcggc ttgacgccct
      901 acgaggtgct gttgtcgctg ttcgcccgcg ccgcggatca ttcggccgga cggcagtttc
      961 cgcatcacta cgccagccgg cggctggggc tgcagacgat cagctcggtg attgcggctc
     1021 agcttccgca cgccgtcggg gccgcctacg ccctggcgta tcgcggcgag cgcggacgag
     1081 cggtgctcac caccttcggc gacggtgcga ccagcgaagg cgagtggcac gaatcgctca
     1141 acttcgcggc ggtccactcg ctgccgatcg tctttctctg cgagaacaac gagtgggcga
     1201 tctcgacgcc gctttccaag cagacggccg aacccgacat ctacaaacgc gccgagggct
     1261 acgggatgcc gggcgtacgg gtcgacggga tggatccgat cgcgtgctac gccgccgtca
     1321 agctggccat ggaccgggcg cgttcgggcg caggcccgac gctggtggaa gccaagtgct
     1381 atcggttcct ctcccatacc accgacgacg acgaccgcac ctaccgttcc cgcaccgagg
     1441 tcgaggagcg ccggcggaac gatccggtcc cgcgcttcga agcgctgctg atcgagcggg
     1501 gcatcctcac cccggaggcg gcggagtcct tgaagcgttc cgtcctagaa gaagcgaacg
     1561 aagctaccga ccgggcggag acgatgccct acgtcgaccg gcagcagctc ttcgaagaag
     1621 tttacgccga cggctggcag ccctggcgat gacggcgctc acggcgacga ccacatacga
     1681 ggcggcacac ggatatggct aagacgaaca cgcaggccca gaccctcgtc gagcgctacg
     1741 ggttgaccga cgagcaactg cggcaaatct ttcgcaacat gctgctgcag cggcagctcg
     1801 acaaccgtgg ttttcagctc aaccggcaag gcaaggttcc gttcgcgctc ggcagcgagg
     1861 ggcacgaagc ccttcaggcg ggcgccgcga tggcgttcga gcgcgggaaa gacatcctgg
     1921 cgccgtacta tcgcgatttg gggctgtcgc tcgggatcgg cttgacgccg ttcgagatcc
     1981 tgctgtcgat cttcgcgcgc gcggccgatc acaacggcgg ccgtcaattc ccgaaccact
     2041 attcgtccaa ggccgccggc ttgatgtcgt tttcgtcgat cttagccgca catctgccgc
     2101 acgcggtcgg cgccgcctac gcgctggcat accgcggcga aacgggacgc gccgtgctcg
     2161 caacctgcgg cgacggcacg accagcgaag gcgaatggca cgagtcgatg aatttcgcgg
     2221 cgattcacaa gctgccgttc gtcatgctgg tcgagaacaa cgagtgggcg atctcgacgc
     2281 cgctttccaa acagatggcc cagcccgaga tctacaagcg cgccgccggc tacggcatgc
     2341 ccggccggcg tttcaacgga ttcgatccgg tcgaaacgta cggtgccgtc aaggaagcga
     2401 tggaacgcgc ccgttccggc ggcggcccgt cgctgatcga gggaacttgt taccggtatt
     2461 tggcacattc caccgacgac aacgacatga cgtatcgcac ccgcgaagac gtgctcgagc
     2521 atcgtaaact cgatccggtt ccgcgtttcg aagcggtgct cgtcgaaaac gggatcatga
     2581 ccgaacccga gatcgaggcg atgcgcaagg acgtgctccg cgaaaccaac gaggcgaccg
     2641 acgcggctga agccatgccg tacccgcagc cttccgatct ctacacccac gtttacgaag
     2701 gagcctggca gccgtggcag tgagcagcag tacggcgtcg accgtgatga acaacgtcga
     2761 agcggtccgc gccaccctct acgaagccct gaaaaacgac gaccggaccg tcatcatcgg
     2821 cgaagacgtg ggtccgcgcg gcaacgtctt tctcatcacc aaagatttca tc
//
LOCUS       DASUCJ010001246         4641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_380374, whole genome shotgun sequence.
ACCESSION   DASUCJ010001246 DASUCJ010000000
VERSION     DASUCJ010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380374"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFU90_14580"
     CDS             <1..994
                     /locus_tag="VFU90_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5481069.1"
                     /translation="VMLRRAATAAAIAVIALAVTSRARAELPGPLLQFRQEARKLAAR
                     LPAPSALEVLDLSTGYSLGVNAGASMPAASTIKIPVMVEVFEQLQAGRFDLERRVVLR
                     SADKDWGSGDLCDAPAGTSYPVSELLEKMIDVSDNTATNMLIRLVGRKHINASMRDLG
                     LGRTRLADDIRTDGWGIRQRLRTSPADLVRLLTLMARRRLVDEWSSNEMISILEQDQY
                     NTLLPEPLPDDVPVAHKTGSFFDTLNDAGIVYADDAPYVIAVMTTALPSQNLGRSFIH
                     SISRLAYGDEIRLARWRESSGLTFPAMPGAPGAGSPDLRYWSTGLSDGAGTGGR"
     gene            complement(991..1935)
                     /locus_tag="VFU90_14585"
     CDS             complement(991..1935)
                     /locus_tag="VFU90_14585"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5481070.1"
                     /translation="MDLSSTTVYAGNDFRRYEDAKVGLLTHGLQYGTGCFEGIRGYWV
                     PEEEELYLVLLRDHYERLATSAKILTMGLPHGTDELIDITVDLCLRNRFRTDVYIRPF
                     VYKAAEDVGVRLHNVPDAFAIVPIVYTPYLDEGRGLHVCVSSWRRADDTMAPPRAKIT
                     GLYVNSALAKSEAVENGYDEAILLSHDGHVAEGSAENIFLVRRGVLYTPDPSQNVLEG
                     CTRRAIMDIAREEFGLEVVERSIDRGELYAADEVFFTGTAVGVCYVTSIDRRTVGDGR
                     IGPIAKSLAEYYKRVVVGREPRYERWVTRAYASARVKV"
     gene            2058..2777
                     /locus_tag="VFU90_14590"
     CDS             2058..2777
                     /locus_tag="VFU90_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481071.1"
                     /translation="MTGWVLASTTFLASSVEFVEAATIVLAVGYAQGWRAALSGTISA
                     AASLAAIVAVFGPLLRNAASVARIELAVGPFLILFGIAWLRKAVWRYAGRKSMRDEAA
                     IYDRELARLHADREHRLGFAVAFQGVFVEGLEVAVIVVTFGAAGARALEWSTAGAAIA
                     LAAVTIAAAALHRPFARVPENAMKALVGVMLLALGTFWLGDALGIAWWAGDATLFAIA
                     AAYAIVAAGAVVALRRPGVRA"
     gene            2774..2959
                     /locus_tag="VFU90_14595"
     CDS             2774..2959
                     /locus_tag="VFU90_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481072.1"
                     /translation="MKALGEIYAFVTGGSIVAPVGVIAAALVARFGSGLPAGVHAAVF
                     LGTVIVTFFASTLEDAR"
     gene            2988..>4641
                     /locus_tag="VFU90_14600"
     CDS             2988..>4641
                     /locus_tag="VFU90_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3231..3233,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5481073.1"
                     /translation="MKPYRRLTHPLVRDGGSLREATWDEALDRAASALHSARERNGTR
                     TYGMFSCSKATNEVNYVAGKFARVAVGSNNVDSCNRTUHAPSVVGLTTVFGAGGGTSS
                     YEEAERTGVVVLWGSNARETHPIYFHHVLKGIHNGAKLYVVDPRRTGSARWADRWLGL
                     DVGSDIALANAVAREILAAGLENRAFIERATSGFDAYAASLEPYTLDYAHETTGVPAD
                     AIVELARAYAGAERAQLCWTLGITEHHNAVDNVRALINLALLTGHVGRYGSGVCPLRG
                     QNNVQGGGDMGALPDKFPGGQSVADAALRAKFERAWGVTLPPEPGWNLSGMFEAMERG
                     ELDTLYVIGENPAQSEADQRRANALLGNLRSLIVQDIFLTKTGEMADVVLPAASSWCE
                     SEGTVTSSERRVQRVRKALDPPGEAREDSEIMYELARRLGHDWGAASAETIWDECRSL
                     SPWHAGMSYPRLERLGGIQWPCYDENHPGEMYLHSRLWRDPLIGPRAPFSVVEFEPPV
                     DVLDAEYPIRLTTGRLLDSYNTGEQSRRFPSPLRREATIDLSP"
BASE COUNT          763 a         1561 c         1541 g          776 t
ORIGIN      
        1 cgtcatgttg cgccgcgctg caaccgccgc tgcgatagcc gtcatcgccc tggccgtaac
       61 gtcgcgtgcg cgcgccgaac tccccggtcc gttgctccag ttccggcaag aagcgcgcaa
      121 gctcgcggcg cggctgcccg cgccaagcgc actcgaagtg ctcgacctga gcacgggcta
      181 ttccctgggc gtcaatgccg gcgcgagcat gccggccgcc tcgacgatca agattccggt
      241 aatggtcgag gtcttcgaac agctgcaggc cgggcgtttc gacctcgagc gacgcgtcgt
      301 cttgcggagc gccgacaaag actggggctc gggcgacctg tgcgacgctc cggccggcac
      361 gtcgtatccg gtttccgagc tgctcgagaa aatgatcgac gtcagcgaca acacggcgac
      421 gaacatgctc atccgcctag tcggacgcaa gcacatcaac gcctcgatgc gcgacctcgg
      481 gctcggccgc acgcggctcg ccgacgacat ccgcaccgac ggctggggaa tccggcagcg
      541 gctgcgcacg tcgcccgccg acctcgttcg tctcctcacg ttgatggccc gtcgccggct
      601 cgtcgacgag tggtcttcga acgagatgat ttcgatcttg gagcaggacc agtacaacac
      661 gctgctcccc gagccgctgc ccgacgacgt gccggtcgcc cacaaaacgg gatccttttt
      721 cgacacgctc aacgacgccg gcattgttta cgccgacgac gcgccctacg tcatcgcggt
      781 catgacgacg gcgcttccct cgcagaatct cggccgcagc ttcatccact cgatctcgcg
      841 tctggcctac ggcgacgaaa tccggctcgc ccgctggcgc gaaagcagcg gcctgacctt
      901 cccggccatg ccgggtgcgc cgggagccgg ctcgcccgac ttgcgctact ggagcaccgg
      961 gctcagcgac ggcgccggga cgggcggccg ctaaaccttg acgcgggccg acgcgtacgc
     1021 gcgcgtcacc caccgctcgt aacgcggctc gcggccgacc accacccgct tgtagtattc
     1081 ggccaaactc ttcgcgatcg gtccgatgcg cccgtcgccg acggtgcggc ggtcgatcga
     1141 ggtcacgtag cacacgccca cggccgtccc cgtaaagaac acttcgtcgg ccgcgtacag
     1201 ctcgccgcgg tcgatcgagc gctcgacgac ttccaagccg aactcctcgc gcgcgatgtc
     1261 catgatcgca cggcgggtgc agccttccag cacgttttgc gacggatccg gcgtgtagag
     1321 caccccgcgg cgaaccagaa agatgttttc ggccgatcct tcggcaacgt gtccgtcgtg
     1381 cgagagcagg atcgcttcgt cgtaaccgtt ttcgaccgcc tcgctcttgg ccagcgccga
     1441 gttgacgtac agccccgtaa tcttcgcgcg cggcggagcc atcgtgtcgt cggcccggcg
     1501 ccacgagctg acgcagacgt ggagaccgcg cccttcgtcg agatagggag tgtacactat
     1561 cgggacgatg gcgaacgcgt cggggacgtt gtgcagccgg acgccgacgt cttcggcggc
     1621 tttatagacg aagggacgaa tgtacacgtc cgtgcggaag cggttgcgca ggcacaagtc
     1681 gacggttata tcgatcagct cgtcggtgcc gtgcggcagc cccatcgtca aaatcttggc
     1741 cgacgtcgcg agacgttcgt agtgatcgcg cagaagaacg aggtagagtt cctcttcctc
     1801 gggcacccag tacccgcgga ttccttcgaa acatccggtt ccgtattgca aaccgtgggt
     1861 gagcagcccg actttagcgt cctcgtagcg gcgaaaatcg ttgccggcat agacggtcgt
     1921 cgacgaaaga tccacctttg cgctcctcct tggacacgtc cgggtttgcc aggttgcgac
     1981 atcggccgga cgcttcctcc ggccggcaga cgggcgattt ggcgtgcggc caacagccgg
     2041 gtaggacggg ttccgggatg accggatggg tactggcgag cacgacgttt ctggcgtcga
     2101 gcgtggagtt cgtcgaagcg gccacgatcg tgctggcggt cggctacgcc cagggctggc
     2161 gggccgccct gtcgggaacg atttcggcgg ccgcctccct ggccgcgatc gtcgcggtct
     2221 tcggaccgct gttgcgtaac gcggcatcgg tcgcgcgaat cgagctggcg gtagggccct
     2281 ttctcatcct cttcggcatc gcgtggctgc gcaaagccgt ctggcgctac gccggacgca
     2341 aaagcatgcg ggacgaggcg gcgatctacg accgcgagct ggcgcggctg cacgccgatc
     2401 gcgagcaccg cctcggattt gccgtcgcgt tccagggagt gttcgtggaa ggcctcgagg
     2461 tagcggtgat cgtcgtgacg ttcggtgccg ccggcgcccg cgcgctcgag tggtcgacgg
     2521 ccggcgcggc gatcgcgctc gccgccgtaa cgatcgcggc ggcggcactg caccgtccct
     2581 tcgctcgcgt ccccgaaaac gccatgaagg cgctggtcgg cgtcatgctg ctcgcgctgg
     2641 gaaccttttg gctcggcgac gcactcggaa tcgcgtggtg ggcgggggat gcgacgctgt
     2701 tcgcgatcgc cgccgcctac gcgatcgtcg ccgccggagc ggtcgtcgcg ctgcggcgcc
     2761 cgggcgtgcg ggcgtgaaag cactcggcga aatctacgcg ttcgtcaccg gcggctcgat
     2821 cgtcgcgccg gtgggcgtga tcgcggccgc gctggtcgcc cgcttcggca gcgggttgcc
     2881 tgccggcgtc catgccgccg tgtttctcgg cacggtgatc gtgacgttct ttgccagcac
     2941 gctggaagac gcgaggtaag gccgcgcgta cggcaaagga aatcgctatg aaaccttacc
     3001 ggcggctgac gcatccgctc gttcgcgacg gcggttcgtt gcgcgaagcg acgtgggacg
     3061 aggcgctcga tcgcgctgcg tccgcgttgc actccgcgcg cgaacgcaac ggcacgcgaa
     3121 cctacggaat gtttagctgt tcgaaggcga ccaacgaggt gaactacgtc gctggaaagt
     3181 tcgctcgcgt cgccgtcggc agcaacaacg tggacagctg caaccgaacg tgacacgccc
     3241 cgagcgtcgt cggtctgacg acggtcttcg gagccggcgg cggcaccagc tcgtacgaag
     3301 aggctgagcg caccggcgtc gtggtgctgt gggggtcgaa tgcgcgcgag acgcatccga
     3361 tctacttcca tcacgtcctc aagggaatcc acaacggtgc gaaactctac gtcgtcgacc
     3421 cgcggcgcac gggctcggcc cgctgggccg accgctggct cgggctcgac gtcggttccg
     3481 atatcgcgct ggccaacgcg gtcgcgcgcg aaattctggc ggccggcttg gaaaaccgcg
     3541 cattcatcga gcgcgctacc agcggcttcg acgcctacgc cgcctcgctc gagccgtaca
     3601 cgctcgacta cgcgcacgag acgaccggcg tgccggcgga tgcgatcgtc gaactggcac
     3661 gcgcgtacgc cggcgcggag cgcgcgcagc tgtgctggac gctcggcatc accgagcacc
     3721 acaacgccgt cgacaacgtg cgcgcgctga tcaacctcgc gctccttacc ggccacgtcg
     3781 gccgctacgg atcgggcgtc tgtccgctgc gcggccaaaa caacgttcaa ggcgggggcg
     3841 acatgggcgc actgcccgac aaattccccg gcggccagag cgtcgcggac gcggccctgc
     3901 gcgcgaaatt cgagcgcgcc tggggcgtca cgcttccgcc ggaacccggc tggaacttga
     3961 gcggaatgtt cgaggcgatg gaacgcggcg agctcgacac gctttacgtg atcggcgaga
     4021 atcccgccca gtcggaagcc gaccagcgcc gcgccaacgc cttgctcggc aacctgcgct
     4081 cgctgatcgt ccaagacatc ttcctcacga agaccggcga gatggccgac gtcgtgctgc
     4141 cggcggcgtc gagctggtgt gaaagcgaag gaacggtgac cagcagcgag cgccgcgttc
     4201 agcgcgtgcg caaagcgctc gatcctccgg gagaagcgcg cgaagattcc gagatcatgt
     4261 acgagttagc ccgccgcctc ggacacgact ggggagcggc gtccgccgaa acgatctggg
     4321 acgaatgccg ctcgctttcg ccgtggcacg ccgggatgag ctatccgcgg ctggaacgtc
     4381 tcggcggcat tcagtggccg tgttacgacg aaaaccatcc cggggaaatg tatctgcaca
     4441 gccggttgtg gcgtgatccg ctgatcggtc cgcgcgcgcc atttagcgtc gtggaattcg
     4501 aaccgcccgt ggatgtgctg gacgcggaat atccgattcg cctgaccacg ggacgactgt
     4561 tggattccta caataccggc gaacagagcc ggcggtttcc ttcgcctttg cgacgcgagg
     4621 caaccatcga cctctctccg g
//
LOCUS       DASUCJ010001247         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_899875, whole genome shotgun sequence.
ACCESSION   DASUCJ010001247 DASUCJ010000000
VERSION     DASUCJ010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899875"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1658
                     /locus_tag="VFU90_14605"
     CDS             168..1658
                     /locus_tag="VFU90_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481074.1"
                     /translation="MVLALVLAIAPAGCHGGAAIPPATLPAAARVAPHGAASIVVEPS
                     KVGAPVSKLVMGANMATWYDVTQPGLTPAFKTSGMTATRWPGGSESDNFHWKTNSLGP
                     GSCSGGYVNPNSTFGNFIDDIAHAARLDVAITVNYGSNPPCTAGADPAEAAGWVDYAN
                     RTKHLGVQWWSVGNEQYGFWETDLHSKPHDPGQYAANVASGFYPKMKAASPTPIHVCV
                     DVDPNTAGWDATVLAQAKYDCVELHYYPQGTTVNDAFLIRKAAPLLSAYLAALKAELK
                     AAGRAKTPIYVGEIGSTYGTPGKQTMSITQALYAGQVLGELLEAGVTRATWWLGYGGC
                     NAKDGGGDFSKTLYGWQDFGGFMIFSDGTVEDGCSTTDVPRGTPLPTARAFQVASHFV
                     RDGEHVLGLVKTSLPDVRAYASTYAGGYALLLINLNENAAVDVPVKIAGKTSGEGGSI
                     VVYDKARYDASRRNVWKAPSAAKLGAWSGGFTVTLPPWSIAAVQTK"
     gene            1667..2437
                     /locus_tag="VFU90_14610"
     CDS             1667..2437
                     /locus_tag="VFU90_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5481075.1"
                     /translation="MTRECVRAGGRDLEYVKIPAAREGLPVLVFLHEGLGTVALWREF
                     PAALAERTGCGALVYSRYGNGFSTPLAQPRTPGYMHDEALAVLPEVLRELDVGETILV
                     GHSDGASIALIYAAAHPAAVRGLVLEAPHVFVEELSVQSIAAVKGEYENGLRERMAGH
                     HADVDRTFYGWNDIWLAPEFRDWNIETEVRRVRTPAFVLQGRKDQYGTPAQVEAIAAG
                     SSARVDSLLLAGCAHAPHRDRRALVEETAAAWIRERIA"
     gene            2443..2874
                     /locus_tag="VFU90_14615"
     CDS             2443..2874
                     /locus_tag="VFU90_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5481076.1"
                     /translation="MRPYILLVDDRADDVAVTRRALADNRIVNEVVVLRDGSAACEYL
                     FGAGNETRELPAVILLALKLPKIAGLDVLERIRSDPRTRRLPTVILTSSKNEEDALES
                     RFQSGHGYVRKPIDFSELVQAVHSAGLKWLLIDDSQAPGPR"
BASE COUNT          554 a         1013 c         1103 g          509 t
ORIGIN      
        1 agtacggacc ccagcgcccc caatgctctt ccttgcggcg tcgccgttcg aggcgttcgc
       61 gctccgcgtt catgtgaggt ggctgttcgg cttcgcccgc gcgctccatc ctgcgccgcg
      121 gcgagggttt tggagcaacc gcacggatat agaacggcat gcggcggatg gttctcgcgc
      181 tggtcttagc gatcgcgccg gcgggatgtc acggcggcgc ggcaattccc ccggcgacgc
      241 tgcctgcggc cgcgcgggtc gcgccgcacg gcgcggcgtc gatcgtggtg gagccgtcga
      301 aagtcggcgc cccggtctcc aaactcgtaa tgggcgccaa catggcaacg tggtacgacg
      361 taacgcagcc cggcttgacg ccggcgttca aaacgtcggg gatgacggcg acgcgctggc
      421 cgggcggctc ggagtcggac aacttccact ggaagaccaa tagcctcgga ccgggatcct
      481 gcagcggcgg ttacgtcaat ccgaactcga ccttcggcaa cttcatcgac gacatcgcgc
      541 acgccgcccg tctcgacgtc gcgatcaccg tcaactacgg atcgaatccg ccgtgcacgg
      601 cgggggcgga tccggccgaa gcggcgggct gggtcgatta cgccaaccgc acgaaacatc
      661 tcggcgtcca gtggtggagc gtcggcaacg aacagtacgg gttttgggaa accgatctcc
      721 attccaagcc gcacgatccc gggcagtatg cagccaacgt cgcaagcggc ttctatccga
      781 agatgaaggc ggcgtcgccg acgccgattc acgtgtgcgt cgacgtcgac ccgaataccg
      841 ccggatggga cgcgaccgtc ctggcgcagg caaagtacga ctgcgtcgag ctgcactact
      901 atccgcaggg gacgacggtt aacgacgcgt ttttgatccg taaggcggca ccgctgctaa
      961 gcgcatacct cgcagcgctg aaagccgagc tgaaggccgc cggccgcgcc aagacgccga
     1021 tttacgtggg cgaaatcggc tcgacgtacg gcacgcccgg aaaacagacg atgtcgatca
     1081 cccaggcgct gtacgccggg caagtgttag gcgagctgct cgaagcgggc gtgacgcgcg
     1141 cgacctggtg gctcggctac ggcggctgca acgccaaaga cggcggcggc gatttcagta
     1201 agacgctgta cggctggcaa gacttcggcg ggttcatgat cttctccgac ggaaccgtcg
     1261 aagacggctg ctcgaccacc gacgttccgc gcgggacgcc gctgccgacc gcgcgggcgt
     1321 ttcaagtagc gtcgcacttc gtgcgcgacg gcgagcacgt cctcggcctg gtcaagacgt
     1381 cgctgcccga cgtccgcgcg tacgcttcga cctacgccgg cggctacgcg ctgctgctga
     1441 tcaacctcaa cgagaacgcg gcggtcgacg ttccggtgaa aatcgcgggg aagacgtcgg
     1501 gagagggcgg ctcgatcgtc gtctacgaca aggctcgcta cgacgcgtcg cgccgcaacg
     1561 tttggaaggc gccgtccgcg gcgaagctgg gcgcgtggag cggaggcttc accgtgacgc
     1621 tgccgccgtg gagcatcgcg gccgtgcaaa cgaaataagt tctccagtga cgcgcgagtg
     1681 cgtgcgtgcc ggcggccgcg acctcgagta cgtcaagatt ccggccgcgc gggagggact
     1741 gccggtgctc gtctttctgc acgaaggact cggcacggtc gcgctgtggc gtgagttccc
     1801 ggcggcgctg gccgagcgca cgggatgcgg cgcgctggtc tactcccggt acggaaacgg
     1861 cttctcgacg ccgttggcgc agccgcgcac gccgggatac atgcacgacg aagcgctggc
     1921 cgtgctgccg gaagtgctgc gcgagctgga cgtcggcgaa acgatcttgg tagggcacag
     1981 cgacggcgcg tcgatcgcgc tcatttacgc cgccgcacat ccggccgcgg tgcgcggcct
     2041 ggtgctggaa gcgccgcacg ttttcgtgga ggaactgtcc gtgcaaagca tcgcggcggt
     2101 caaaggcgaa tacgagaacg ggctgcgcga acggatggcg gggcatcacg ccgacgtcga
     2161 ccggacgttt tacgggtgga acgacatctg gctcgcgccg gagtttcgcg attggaacat
     2221 cgaaacggaa gtccggcgcg tccggacgcc ggcgttcgtg ctgcaagggc gcaaagacca
     2281 gtacggaacc ccggcgcaag tcgaggcgat tgccgccggt tcgtcggccc gggtcgacag
     2341 cctgctgctg gccggctgcg cgcacgcgcc gcatcgcgat cgccgggcgc tggtagagga
     2401 aacggcggcg gcgtggattc gggaaaggat agcgtgaccg caatgaggcc gtacattctg
     2461 ctggtcgacg atcgcgccga cgacgtcgcg gttacgcgcc gcgcgctggc cgacaaccgg
     2521 atcgtcaacg aagtcgtcgt gctgcgcgac ggcagcgcgg cgtgcgagta cttattcggc
     2581 gccggcaacg aaacgcgcga gctgccggcg gtgattttgc tggcgctgaa gctgccgaaa
     2641 attgccgggc tcgacgtgct cgaacgcatc cggtcggatc cgcggacgcg acggctgccg
     2701 accgtcattc tcacgtcgag caagaacgag gaggacgcgc tcgagagccg gtttcagagc
     2761 ggtcacggct acgtgcgcaa accgatcgac ttcagcgagc tggtccaggc ggtgcattcc
     2821 gccggcctca agtggttgct gatcgacgac tcgcaggcac ccggcccgcg ataacagcga
     2881 cacggcccac ggttgctttt gcgatttccg cgacgcagcg gcgcaagaaa acgttaaagc
     2941 gtcttttcct tttgcgatgg tataatacgg gccgcgcacg aacgaccggc gaggtgggtt
     3001 ggtttgcagt ctcagacgca tacgttagcg agccgcgcag atctgcggtc cttcctgacc
     3061 ttttgccggg cccggttgca ccccgaagac gtcggcctcc gcaacaccca tcgacgccgg
     3121 gtcccgggac tgcggcgcga ggaagtcgcc gagctcgtgg gcgtgtccgc tcgctggta
//
LOCUS       DASUCJ010001248         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1404075, whole genome shotgun sequence.
ACCESSION   DASUCJ010001248 DASUCJ010000000
VERSION     DASUCJ010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404075"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..431)
                     /locus_tag="VFU90_14620"
     tRNA            complement(358..431)
                     /locus_tag="VFU90_14620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Gly,seq:tcc)
     gene            complement(441..525)
                     /locus_tag="VFU90_14625"
     tRNA            complement(441..525)
                     /locus_tag="VFU90_14625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Tyr,seq:gta)
     gene            681..1844
                     /locus_tag="VFU90_14630"
     CDS             681..1844
                     /locus_tag="VFU90_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481077.1"
                     /translation="MRRFRVLAMVAAAVFALAGCTAGDGQGGSAIPLGGAETVGDAAA
                     IMAHRPTDTQTTAAAIGAVGALGSRIAALARTADGDDTGRTIGACRDGRQFFAPDRRG
                     DARSTETRDYFDSACTQLARDTLRLYAFRAPAGEVVARVISLYQRGSSTPVAVRRETS
                     FVASTRFGRWGFPVARDGFAQTTASSLWVGNRRQSSDGSGTVLLPGRGVTTGYCQDSA
                     GYSVGGFPSLDATFGWEGGTLDSQISATRTTEGDGAITLASIEQGETFAGPIGSLAAV
                     GQKHAGSCPSASPAYRLQGGELAGAFIAPIRATYRGGRLRRLSVSGASFSGGYRLNAV
                     TTTAGLQAPRVAGTLAGGGFRVAAFETDAFGNGTLTIASTGAQYRLIDWTIVR"
     gene            1872..1953
                     /locus_tag="VFU90_14635"
     tRNA            1872..1953
                     /locus_tag="VFU90_14635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1906..1908,aa:Thr,seq:tgt)
     gene            1977..2318
                     /locus_tag="VFU90_14640"
     CDS             1977..2318
                     /locus_tag="VFU90_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481078.1"
                     /translation="MQNVTPKWRARGLVLVCERCSKERIPEEDPATAERIGDFHLRNW
                     LKAKLKEDGRWGPIRAINTSCMDVCARRHVTVAIEPERGEPQVFVVDPLDDKDELYAK
                     IIELLATPPPP"
BASE COUNT          441 a          854 c          779 g          459 t
ORIGIN      
        1 gttctgggat ccgccgcaac ccgcgatcat ggcggtggct gataatgcgg cgagacttgg
       61 aagcgggact cggccaaagg gaaatgtgcg catgagttac gcctcttaac ctagggcgaa
      121 gcggcgtggc ccccactcag ccaagcaggc gtcctagagc ctgcattata cactcggcta
      181 aagccaaacg catttcgtgc ccgccggagg cctgaaattc taatcctggg gctgttactc
      241 agcctccagg cgcatcgccg tcgcgaactg ctcgggcgtc atgtagcccg aggtcgtcca
      301 tcatccgaac gaactcggcg atcctctgat gacgctctcg caatgcaccc gaagttttgg
      361 agcgggcggc gggaatcgaa cccgcgactc ttgcttggaa ggcaagggca ttaccactat
      421 gcaacgcccg cgatgggaat tgggcagggc tggattcgaa ccagcgtagc gctttcgcgc
      481 gccaagttta cagcctggtg ccattaaccg ctcggccacc tacccacgaa tcggcagacc
      541 cgggtacgat accgcacggg gggagtcctc gcaagcgctt cggccgtcct cctctcacct
      601 ttttctcaca atatcggcat atggttcgct taccatagcg ggtgagggtt cccccggtcg
      661 actgcgagga gcgttttggc atgcgccgtt tccgggtttt ggccatggtc gcggcggcag
      721 ttttcgcgtt ggcgggctgc acggccggcg acggccaggg cggttccgcg attccgctcg
      781 gcggcgccga aacggtcggc gatgcggccg caattatggc gcatcgtccc accgacacgc
      841 aaaccaccgc cgccgctatc ggtgcggtcg gcgccctcgg ctcgaggatc gccgcgctcg
      901 cccgcaccgc cgacggcgac gataccggcc gcaccatcgg cgcctgccgc gacggacggc
      961 agttctttgc gcccgaccgc cgcggcgacg cccgctctac cgaaacgcgc gactacttcg
     1021 attcggcctg cacgcagctc gcgcgcgaca cgttgcgcct ctacgctttc cgcgctccgg
     1081 ccggtgaagt cgtcgcgcgc gtgatctcgc tctaccagcg gggaagttcc acgccggtcg
     1141 cggtccgccg cgaaacgtcg ttcgtcgcga gcacgcgttt cggacggtgg ggatttccgg
     1201 ttgcgcgcga cggcttcgca cagacgaccg cgagttcgct ctgggtcggc aaccgccggc
     1261 agagctccga cggcagcggc accgtgctgc ttccgggtcg cggcgtaacg accggatatt
     1321 gccaagattc ggccggctac agcgtcggcg gtttcccgtc gctcgacgcc accttcggct
     1381 gggaaggcgg cacgctcgat tcgcagattt ccgccacgcg cacgaccgag ggcgacggtg
     1441 cgatcacgct ggcgtcgatc gagcagggtg aaacgtttgc cggcccgatc ggttcgctcg
     1501 ctgccgtcgg gcagaagcac gccggctcgt gcccgtccgc gtcgccggcc tatcgtttgc
     1561 aaggcggcga gctagccggc gcgtttatcg cgccgattcg cgcgacctac cgcggcggac
     1621 ggctccgccg cttgtcggtg agcggcgcgt cgttcagcgg cggctaccgg ctcaacgccg
     1681 tcacgacgac ggccggcctg caggcgccgc gcgtggccgg cacgctcgcc ggcggcggct
     1741 tccgcgtcgc cgcgttcgaa accgacgcgt tcggaaacgg cacgctgacg atcgccagca
     1801 ccggcgcgca gtaccggctg atcgactgga cgatcgtccg ctaacgtgct acagtcaccg
     1861 ctatcgtcgc cgccgacgtg gctgagcggt tgaaggcgcg tgatttgtaa tcacgtccga
     1921 aaggcatcgc cggttcgaat ccggccgtcg gcttagcact ttcctgcaga gattcgatgc
     1981 aaaacgtcac cccgaaatgg cgcgcgcgcg gcctagtgct cgtatgcgag cgctgctcta
     2041 aggagcgcat ccccgaagag gatccggcga ccgccgagcg gatcggcgac ttccatttgc
     2101 gcaactggct caaagccaag ctgaaagaag acggccgctg ggggccgatt cgcgcgatca
     2161 acaccagctg tatggacgtc tgcgcgcgcc gtcacgtcac cgtcgcgatc gaacccgaac
     2221 gcggcgaacc gcaggtcttc gtcgtcgacc ccctcgacga caaagacgaa ctctacgcca
     2281 agatcatcga actcctagcc accccacccc caccctagtc ccttccctca aagaagaact
     2341 tgtcggcttc gagtttcagt ggtttgagct cgcctctgag atcgctctta gaaatctctc
     2401 tctttttctt ccaaactccc aatagcaaag gctccggtat tatcctaaaa aaattttggt
     2461 cactctcgcg aagtacgaca aaattttttt agggtgatac cggagcctaa gcgctcctag
     2521 ataccggaac ccc
//
LOCUS       DASUCJ010001249         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_838520, whole genome shotgun sequence.
ACCESSION   DASUCJ010001249 DASUCJ010000000
VERSION     DASUCJ010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838520"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1161)
                     /locus_tag="VFU90_14645"
     CDS             complement(<1..>1161)
                     /locus_tag="VFU90_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5481079.1"
                     /translation="MGGPVWGWFTGWFNLIGQIAITAGIDYGAATFTDALLQLLWPGT
                     FKGTQHEIVYVYAVILALHAVMNIFSVKLVAFLNDVSVWWHVAGVLIIVGFLIFKPSH
                     HQSFNYVFGHTINNSGFGHSWLWFVLLLGLLQAQYTYTGYDASAHMSEETQDASRSAA
                     RGVIGSIVVSAVFGFILALGVTFAIQNQGATTGAGGFAVKQVFLDSLGSGAAKFVLFI
                     CVGAQFYCGMSSITSASRMMYAFSRDGAVPGHQLWRRLNRERVPYMAAIAIAVLAFLC
                     AFPAYFGTNGVVAYVAVTSIATIGLYIAYAIPIFLRLRAGDAWEPGEWNLGRHYKWIG
                     WTAVIWVGFISVLFILPTSPGGVPWRSEFSWVLFNYAIVAVGGTLLLVGGWWM"
BASE COUNT          237 a          385 c          373 g          166 t
ORIGIN      
        1 catccaccag ccgccgacga gcaacagcgt gccgccgacc gcgacgatcg cgtagttgaa
       61 gagcacccag ctgaactcgc tccgccacgg aacgccgccc ggggaggtcg ggaggatgaa
      121 gaggacgctg atgaagccga cccagatgac ggccgtccag ccgatccact tgtagtgccg
      181 gccgaggttc cattctcccg gctcccatgc atcgcccgcg cggagccgca ggaagatcgg
      241 gatcgcgtac gcgatgtaga ggccgatcgt ggcgatcgac gtgaccgcga cgtaggcgac
      301 gacgccgttc gtcccgaagt acgccgggaa cgcgcacagg aacgcgagca cggcgatcgc
      361 gatcgccgcc atgtagggga cacgctcgcg gttcagccgc cgccagagct ggtgccccgg
      421 cactgcgccg tcgcgcgaga acgcgtacat catccgcgac gccgacgtga tcgacgacat
      481 gccgcagtag aactgcgccc cgacgcagat gaagagcacg aacttcgccg ctcccgaccc
      541 gagcgagtcg aggaacacct gcttcaccgc gaagccgcct gcgccggtcg tggcgccctg
      601 gttctggatc gcgaacgtga caccgagcgc gaggatgaag ccgaagacgg ccgacaccac
      661 gatcgagccg atgacgccgc gtgccgccga gcgagacgcg tcctgcgtct cttcgctcat
      721 gtgcgccgac gcgtcgtagc cggtgtaggt gtactgcgcc tgcagcagac cgaggagcaa
      781 cacgaaccac agccagctgt gaccgaagcc gctgttgttg atcgtgtggc cgaacacgta
      841 gttgaacgac tgatggtggc tcggcttgaa gatcaagaac ccgacgatga tcagcacgcc
      901 ggcgacgtgc caccacaccg agacgtcgtt gaggaacgcg acgagcttga cgctgaagat
      961 gttcatcacc gcgtgcagcg ccaggatgac ggcgtacacg tagacgattt catgttgcgt
     1021 gcccttgaac gtgccgggcc acagcaactg cagcaacgcg tcggtgaacg tcgcggcgcc
     1081 gtagtcgatc ccggctgtga tcgcgatttg cccgatcagg ttgaaccatc cggtgaacca
     1141 accccacact gggccgccca t
//
LOCUS       DASUCJ010001250         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_181301, whole genome shotgun sequence.
ACCESSION   DASUCJ010001250 DASUCJ010000000
VERSION     DASUCJ010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181301"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="VFU90_14650"
     CDS             complement(<1..>1007)
                     /locus_tag="VFU90_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481080.1"
                     /translation="SRVDDLIARGASPDDAYAEALRRLGGSLDDARLQLHTSAKRRER
                     RMRFSQFIDSAAQDIQYAARGLIRRPAFTIVSVLTLALGIGGTTAIFSAVNAMLLRPL
                     PYARPNELMQIRLILPAEGGRPASPMGWSYPMFSMLRETQHVLADLAVYSPSQFTLTA
                     GDVERVTGEYVGASYLRVLGLSPSRGRDFDRAADAHSGATHEAIVSYALWQRRFNADP
                     SVIGRTLDIDREPWTIIGVGPSGFRGLSGQADIFLPAMTEPADRLTAAWFPFWLVARR
                     APGVTAAEASAAVSVLGARVASAFPNPMGKVNWEVNASPLDDGRLEPALKRSLLMLFG
                     AV"
BASE COUNT          178 a          328 c          346 g          155 t
ORIGIN      
        1 actgcaccga acagcatcag gagcgagcgc ttgagcgccg gctcgagccg cccgtcgtcg
       61 agcggcgacg cgttcacttc ccagttcacc ttacccattg ggttcgggaa ggcgctggcg
      121 actcgtgcgc cgaggacact gaccgcggca gaggcttcag ccgccgtaac tccaggtgcg
      181 cgacgggcga ccaaccagaa tggaaaccac gctgcggtca gtctgtccgc cggctccgtc
      241 atagccggaa gaaagatgtc cgcctgaccc gagagaccgc gaaagccgct cgggccgacg
      301 ccgatgatcg tccacggctc gcggtcgata tcgagggttc gtccgatgac cgacggatcg
      361 gcgttgaagc ggcgctgcca gagcgcgtag gacacgatcg cttcgtgcgt cgctcccgag
      421 tgcgcgtcgg ccgcgcgatc gaagtcgcgt ccacgcgacg gcgacaatcc caagacacgc
      481 aggtacgacg cgccgacgta ttccccggtg accctttcga cgtcgccggc ggtcagagtg
      541 aattgcgacg gcgagtacac cgccaagtcg gcgagtacgt gctgtgtttc gcgcagcatg
      601 ctgaacatcg gatacgacca acccatcggg ctggcgggcc gcccgccctc ggccgggagg
      661 atcagtcgga tctgcatcag ctcgttcggc cgcgcatagg gcagcgggcg gaggagcatg
      721 gcgttgacgg cgctgaagat cgccgtcgtg cctccaatgc cgagggcgag cgtgagcacc
      781 gacacaatcg tgaatgccgg ccggcgaatc agtccgcggg cagcgtactg gatgtcctgc
      841 gccgcactat cgatgaattg gctgaaacgc atacggcgct cccggcgttt ggcggacgtg
      901 tgtagctgca ggcgggcgtc gtcgagcgag ccgccgagtc gtcgcagcgc ctcggcgtag
      961 gcgtcgtctg gagacgcccc ccgcgcgatg agatcgtcga cgcggct
//
LOCUS       DASUCJ010001251         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_89670, whole genome shotgun sequence.
ACCESSION   DASUCJ010001251 DASUCJ010000000
VERSION     DASUCJ010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89670"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VFU90_14655"
     CDS             complement(<1..1559)
                     /locus_tag="VFU90_14655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5481081.1"
                     /translation="MRRHHPRGAHVDSAVRPLLDSVRARNPKASIALIEHAYEVARDA
                     HKDQVRRSGDPYIAHPLGVAMILSELGLDEVTLAGALLHDAVEDTSVTLERLEAEFGP
                     DVSAIVDGVTKLDRLQFDSREAQQAATMRKMLVAMAKDVRVLLIKLADRLHNMRTIAS
                     LPEAKQKRIAQETLDIYAPLAHRLGIQDVKWQLEDLAFAVLHPKRYAEIEAMVLQHAG
                     EREAYLDDVIAAVRARLDQLHIEAEVRGRPKHYWSIYEKMVVRGKEFDEINDLVGVRV
                     VVDSVKDCYGALGSIHALWRPVQGRFKDYIAMPKFNLYQSLHTTVVGPQGRPVEFQIR
                     TVEMHRRSEFGVAAHWGYKEKQSPAEDLVWLQRIVDWQQETTDPAEFMQSLKIDLEQD
                     EVFIFTPKGKVITLPTGATPVDFAYAIHTEVGHRCIGARVNGRLIPLDAALQSGDTCE
                     IFTSKVEGAGPSRDWLQFAQTPRARSKIRQWFSRERRVDAIDTGRDELVKALRREGLP
                     VQKLTNSPTLLT"
     gene            complement(1608..>2066)
                     /locus_tag="VFU90_14660"
     CDS             complement(1608..>2066)
                     /locus_tag="VFU90_14660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5481082.1"
                     /translation="FKDITPLLAAPDVFAATVDALAAPFADVAIDKVLGIEARGFVFA
                     SPVAYRHAAGFVPVRKAGKLPWEIEREEYELEYGTDLLEIHRDAVAPGERVLIVDDVI
                     ATGGTAAATARLVERLGGTVLGLAFVLELGFLGGRKKLGAYNVQSLVTYA"
BASE COUNT          356 a          728 c          627 g          355 t
ORIGIN      
        1 gtgagcagcg tcggtgagtt ggtcagcttc tgtaccggga gcccttcgcg gcgcagcgcc
       61 ttgacgagtt catcgcggcc ggtgtcgatc gcgtcgacgc ggcgctcacg cgagaaccat
      121 tgccggatct tcgaccgcgc acgcggcgtc tgcgcgaact gcaaccagtc gcggctcggg
      181 cccgcgccct ctaccttgct cgtgaatatc tcgcacgtat cgccggactg caacgcggca
      241 tcgagcggaa tcagccggcc gttcacacgc gcgccgatac agcggtggcc gacctcggtg
      301 tgaatcgcgt acgcgaagtc gaccggcgtc gcacccgtcg gcaacgtgat gactttgccc
      361 ttcggcgtga agatgaagac ctcgtcctgt tcgaggtcga tcttcaagct ctgcatgaac
      421 tcggccgggt cggtcgtctc ctgttgccag tcgacgatcc gttgcaacca cacgaggtct
      481 tcggccgggc tctgcttctc cttgtagccc cagtgcgcag cgaccccgaa ctccgaacgc
      541 cggtgcatct cgacggtacg gatctggaac tcgaccggac gcccctgcgg cccgaccacc
      601 gtcgtatgca acgactggta gaggttgaac ttcggcatcg cgatgtagtc cttgaagcgg
      661 ccctgcaccg gacgccacaa cgcgtggatc gaaccgagcg cgccgtaaca gtccttcacg
      721 gaatcaacga ccacgcgcac accgacgagg tcgttgatct cgtcgaactc cttgccgcgc
      781 accaccatct tctcgtagat cgaccagtag tgcttcggcc ggccacgcac ctcggcttcg
      841 atgtgcaact gatcgagccg ggcgcgcacc gcggcaatga cgtcgtcgag gtatgcctca
      901 cgctcacccg cgtgctgcaa caccatcgcc tcgatctcgg cgtaacgctt cgggtgcaac
      961 accgcgaacg cgagatcttc gagctgccac ttgacgtctt gaatgccgag ccggtgcgcg
     1021 agcggcgcgt agatgtcgag cgtctcctgc gcgatgcgct tctgcttcgc ttcgggcaac
     1081 gacgcgatcg tccgcatgtt gtgcaggcgg tcggcgagct tgatcagaag gacacgaacg
     1141 tccttcgcca tcgcgaccag catcttgcgc atggtcgccg cctgctgcgc ttcacgagag
     1201 tcgaactgga gacggtcgag cttcgtgacg ccgtcgacga tcgcggaaac gtcgggaccg
     1261 aactccgcct cgagacgttc gagcgtcacc gaagtgtcct cgaccgcgtc gtgcaacaac
     1321 gcgcccgcca gcgtcacttc gtcgagtccg agctccgaaa ggatcatcgc gacgccgagc
     1381 ggatgtgcga tgtacggatc gcccgaccgg cgaacttggt ccttgtgggc gtcgcgcgcg
     1441 acctcgtacg cgtgctcgat cagcgcgatc gatgccttcg gattgcgcgc ccgcaccgag
     1501 tcgagcagcg gccgcaccgc cgaatcgacg tgcgcgcccc tcgggtggtg cctacgcacg
     1561 atcgtcgact tcaacgggtc cactcccctc tatcggcagc cgcagcgcta cgcatacgtc
     1621 accaaagact gaacgttgta cgcaccgagc ttcttccggc cgcccaagaa cccgagctcc
     1681 agcacgaacg ccagccccag caccgttccg cccagccgct cgaccagacg ggccgtcgcc
     1741 gccgccgtcc cgccggtcgc gatgacatcg tcgacgatca gcacccgttc gccgggcgcg
     1801 accgcgtcac ggtgaatctc gagcaagtcg gtgccgtact cgagctcgta ctcctcacgc
     1861 tcgatctccc acggcaactt gccggctttg cggaccggca cgaaacccgc ggcgtggcgg
     1921 tacgccaccg gcgacgcgaa cacgaacccc cgcgcttcga tcccgagcac cttgtcgatc
     1981 gcgacgtcgg cgaacggcgc agcgagcgcg tcgacggtcg ccgcgaagac gtcaggtgcc
     2041 gcgagcagcg gcgtgatgtc cttgaa
//
LOCUS       DASUCJ010001252         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_472045, whole genome shotgun sequence.
ACCESSION   DASUCJ010001252 DASUCJ010000000
VERSION     DASUCJ010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472045"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /locus_tag="VFU90_14665"
     CDS             <1..>1728
                     /locus_tag="VFU90_14665"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481083.1"
                     /translation="VIYAGTGEADIRGDFDTGDGVYKSTNAGKTWTYAGLRDTHMTSA
                     IVVDPRDPNVVYAASMGHVFKPNPERGIFKSTDGGKNWRKVLFVDENTGGVDLAMDPH
                     DPRVLYAAMWQAQRVPWKLTSGGPGSGLYKTTDGGARWTKISANPGFARGILGKIGVS
                     VAASDPRVVYAIVQARDGGVFRSNDAGATWRRVNAEMKLRQRAFYYMAIFVDPTNPQV
                     AYAPQVDGVYKTKNGGKTFNEIDLPHGDNHIVWINPRNPRILLAGNDGGGTVSVDGGE
                     HWSSEDNQPTGQYYHVAIDDQFPFHVFGAAQDEGAYEGPSAALGGGIGPGEWHAVALG
                     ESTFVAPQPGSPFVTYGSGYYSAFVALDRITGEEKNVSPWPRYMPGASSAETKYRFGW
                     THPIFFSPADPRELLVASQVVFSSLDRGRTWSILSPDLTRNDPSTEGPSGGPVDYDQT
                     GAETFPDVASLAVSPLDANVLWAGSADGLVHVTKDHGSHWSLVTPPQLPQWAQISSIE
                     PSHRDKGTAYLTASRYMWDDFHPYVYVTNDYGAHWSALANGLPANQYVFAVRQDPNEP
                     RLLFAGTRTT"
BASE COUNT          326 a          569 c          555 g          278 t
ORIGIN      
        1 gtgatttacg ccggaacggg agaagccgat attcgcggcg acttcgacac gggcgacggc
       61 gtctacaagt cgaccaacgc cggcaagacg tggacgtatg ccgggttgcg cgacacgcac
      121 atgacctccg cgatcgtcgt cgatccgcgc gaccccaacg tcgtctacgc cgcatcgatg
      181 ggacacgtct tcaaacccaa ccccgaacgg ggcattttca aaagcaccga cggtggaaag
      241 aactggcgaa aagtcttgtt cgtcgacgag aacaccggcg gtgtcgatct cgcgatggat
      301 ccgcacgacc cgcgggtgct ctacgccgcc atgtggcagg cgcagcgcgt gccgtggaag
      361 ctcaccagcg gcggaccggg cagcgggctg tacaaaacga ccgacggcgg cgcccgctgg
      421 acgaagatct ccgccaaccc ggggttcgct cgcggcatcc tcggcaagat cggcgtctcg
      481 gtcgcggcca gcgatccgcg cgtcgtttac gcgatcgtcc aggcgcgcga cggcggcgtc
      541 tttcgctcga acgacgccgg cgcgacctgg cgccgcgtca acgccgagat gaagctgcgg
      601 cagcgagcct tctactatat ggcgatcttc gtcgacccga cgaatccgca ggtggcctac
      661 gcgccccaag tcgacggcgt atataagacg aagaacggcg gcaaaacctt taacgagatc
      721 gatctgccgc acggcgacaa tcacatcgtg tggatcaatc cgcgcaaccc gcgcattctg
      781 ctggccggaa acgacggcgg gggaaccgtt tcggtcgacg gcggcgagca ttggagctcc
      841 gaagacaatc agccgaccgg acagtactat cacgtcgcaa tcgacgatca gttcccgttc
      901 cacgtcttcg gtgcggcgca ggacgagggc gcctacgaag ggcccagcgc cgcgctcgga
      961 ggcgggatcg gaccgggcga atggcacgcg gtagcgctgg gtgagagcac cttcgtcgcg
     1021 ccgcagccgg gcagcccgtt cgttacctac gggagcggct actacagcgc gttcgttgcg
     1081 ctcgaccgca tcaccggtga ggaaaagaac gtcagtccgt ggccgcgtta catgcccggt
     1141 gcgtcgtcgg cggaaacgaa ataccggttc ggctggacgc atcccatctt cttctcgccg
     1201 gccgatccgc gcgagctgct ggtcgcttcg caagtcgtgt tttcgagtct ggatcgcggc
     1261 cgcacgtgga gcatcctcag cccggacttg acgcgcaacg atccgtctac cgagggcccg
     1321 agcggcggcc cggtcgacta cgatcaaacc ggcgcggaaa cgttcccgga cgtcgcgtct
     1381 ctggcggtat cgccgctgga cgccaacgtg ctgtgggccg gctccgccga cggtttggta
     1441 cacgtcacca aagaccacgg cagccattgg agcctggtga cgccgccgca gctgccgcag
     1501 tgggcgcaga ttagctcgat cgagccgtcg catcgcgaca agggcaccgc ctatctcacc
     1561 gcctcgcggt atatgtggga cgactttcat ccctacgttt acgttacgaa cgattacggc
     1621 gcgcactgga gcgcgctcgc caacggattg cccgcgaatc agtacgtgtt cgcggtacgc
     1681 caagatccca acgagccgcg cttgctgttc gcggggacgc gcaccacg
//
LOCUS       DASUCJ010001253         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_151107, whole genome shotgun sequence.
ACCESSION   DASUCJ010001253 DASUCJ010000000
VERSION     DASUCJ010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151107"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFU90_14670"
     CDS             complement(<1..218)
                     /locus_tag="VFU90_14670"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5481084.1"
                     /translation="MDALAVKPLLDGDPYVGVTRGGTLESVHRVAACAVDRNERTLFA
                     LGSIDVPVYLRSTAKPFIAAAAVREGVV"
     gene            complement(200..>1166)
                     /locus_tag="VFU90_14675"
     CDS             complement(200..>1166)
                     /locus_tag="VFU90_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5481085.1"
                     /translation="PYYWRGKPAIDRLTVRFVPDSNTAINLLHTGEIGGFFDDQDLGN
                     YPLLAGVPHVYVSNEPVNAVGAIIFNTQDPLTRDPRVRHALAEAIDVRSLVAKAYRGA
                     LTSRDAGRGLLIWAYDPQAYPDIPYDPAAAAKLLDAAGWAAGPGGLRVKGGRPLDLQF
                     VIQSQTPVDQVVGNLVAAYERKIGVRVTLKQFAVTQFVAPANLGGPVYGGHFQLALYP
                     FVNGDDPDVTDQFACKNVPPNGYNKSRLCDPAVDALLAAGRRTYAVSARKAIYARLQR
                     LLYAQLPIALLYQNHQISAFTTRLHGQTTSLSGAFWNAGSWTLSP"
BASE COUNT          203 a          346 c          433 g          184 t
ORIGIN      
        1 tcgaccacgc cctcgcgcac cgcggcggcg gcgatgaacg gcttggcggt cgagcgcaga
       61 tagaccggaa cgtcgatcga gccgagcgcg aagagcgtgc gttcgttccg gtcgacggcg
      121 caggcggcga cgcgatgcac cgactcgagc gtgccgccgc gggttacgcc gacgtacgga
      181 tcgccgtcga gcagcggctt tacggcgaga gcgtccacga tccggcgttc cagaaggcgc
      241 ccgacagcga ggtcgtttga ccgtgcaaac gggtcgtaaa ggcgctgatt tgatggtttt
      301 ggtagagcaa cgcgatcggc agctgcgcgt agaggagccg ctgcagacgc gcgtagattg
      361 ctttgcgggc gctcaccgcg taggtgcgcc ggccggccgc gagcagcgcg tcgacggcgg
      421 ggtcgcagag gcgcgactta ttgtagccgt tgggcgggac gttcttgcac gcgaactggt
      481 cggtcacgtc ggggtcgtcg ccgttgacga acggatagag tgcgagctga aagtgaccgc
      541 cgtaaaccgg gccgccgaga ttcgcgggcg cgacgaactg ggtaacggcg aactgtttga
      601 gcgttacgcg cacgccgatc ttgcgctcgt aggcggcgac caggttgccg acgacttgat
      661 cgaccggcgt ttgcgactga atgacgaact gcaaatccaa cggacggccg cctttgacgc
      721 gcagtccgcc gggaccggcg gcccagccgg cggcgtcgag cagcttggcg gcggccgcgg
      781 gatcgtacgg gatgtcggga tacgcctgcg gatcgtaggc ccagatgagc agcccgcggc
      841 cggcgtcgcg gctggtcaac gcgccgcgat acgccttggc gacgagcgac ctaacgtcaa
      901 tcgcttcggc cagcgcgtgg cgaacccgcg gatcgcgcgt gagcggatcc tgcgtgttga
      961 agatgatggc gccgaccgcg ttgaccggct cgttcgagac gtagacgtgc ggtacaccag
     1021 cgagcagcgg atagttgccg agatcctgat cgtcaaagaa gccgccgatc tcgccggtgt
     1081 gcagcaagtt gatcgcggtg ttggaatccg ggacgaaacg gacggtcagc cggtcgatgg
     1141 ccggtttgcc gcgccagtag taggga
//
LOCUS       DASUCJ010001254         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_609085, whole genome shotgun sequence.
ACCESSION   DASUCJ010001254 DASUCJ010000000
VERSION     DASUCJ010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609085"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="sucD"
                     /locus_tag="VFU90_14680"
     CDS             complement(<1..837)
                     /gene="sucD"
                     /locus_tag="VFU90_14680"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5481086.1"
                     /translation="MSIFLDKNSKIIVQGITGAEGSYHTDRMVKYGTNVVGGVTPGKG
                     GQKTPHGLPVFDTVKQAVEATGATHTCIFVPPAFAADALYEAHAAGITLAVCITEGVP
                     VHDVLEVVGTTPGMRIIGPNCPGLISPGKSLVGIMPGHIFKEGEIGLISRSGTLTYEV
                     VDGLTRAGMGQSTCVGIGGDPIIGTTFVDCLREFKNDPQTTALVICGEIGGSDEEDAA
                     AFIRAHMPEMPAVAFVGGRNAPPGKSLGHAGAIISGNFGTAQSKIDAFRAAGVPVADR
                     PSE"
     gene            complement(834..1991)
                     /gene="sucC"
                     /locus_tag="VFU90_14685"
     CDS             complement(834..1991)
                     /gene="sucC"
                     /locus_tag="VFU90_14685"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5481087.1"
                     /translation="MEYQGKELFRRAGIPVPRSRHALTPHDVAEYVGSVAGEWVVKAQ
                     VLMGGRGKAGKIKFANTADEGHKVAEEILATPMPPNRQNPAGEPIKSLLIEEKLPIAK
                     EAYCAIAIDRSAKKPVIMVSRFGGMDIEEVSEKHPESIAKYYVDTAAGYSPFIGRELA
                     FEAEVDPGYRKQLPAILGALYDLFFRYGAKLVEINPLALTGDGRVYASDAKVELDDDA
                     LFRHPEFAQWQQALPLDEDERLAAAAGLGIRNFRRFGGTVGTMANGAGLAMATMDAVS
                     YAGGEVANFLDVGGGANAERVRRCYDLVVNSTGAKVFFINIFGGITRGDEVARGIVDA
                     MRETTARKIPLVIRLTGTNEEEGRRILAEAGMTPVETMDEGARRAVEVAKA"
     gene            complement(2030..>3156)
                     /locus_tag="VFU90_14690"
     CDS             complement(2030..>3156)
                     /locus_tag="VFU90_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5481088.1"
                     /translation="DEQRAIAALTAEIAQREIAPSIARWDREHAFPRELYGKLTEAGI
                     MGIAVPEEYGGAGSGYVSYALAIEELARVDAGTAVTVSVHAMICAAILKRGSDEQKRE
                     WLPKLASEDVIAGFALTESDAGSDAAAIRATATATPGGYRLDGRKQWCTNGSYCGVMM
                     GMFRTGRTGAKGVSAFLIDAATPGVTVEKVTEKLGIHTSNTCDVAFDGAVVPAGALLG
                     AEGAGFGYAMTALTSGRIGIAAQATGILAACLDESVKFAKERTAFGRPIGAFEGVSFK
                     IAQMATDLDAARLLVYRAAALADAGKPFAVEASKAKLFASTAARKHAAEAVQIHGGYG
                     YTTEFPVERYYRDAKITEIYEGTSEIQQLIVARSLLGKLD"
BASE COUNT          471 a         1080 c          994 g          611 t
ORIGIN      
        1 ttccgacggc cggtcggcga ccggaacgcc ggccgcgcgg aacgcgtcga tcttgctctg
       61 cgcggtgccg aaatttccgg agatgatcgc acccgcgtgg ccgagcgatt ttccgggcgg
      121 cgcgttgcgg ccgccgacga acgcgaccgc gggcatctcc ggcatgtgcg cgcggatgaa
      181 cgcggctgca tcttcttcgt cggagccgcc gatctcgccg caaatcacca gtgcggtggt
      241 ttgcggatcg ttcttgaact cgcgcaggca gtcgacgaag gtcgtgccga tgatcgggtc
      301 gccgccgatg ccgacgcagg tcgactgtcc cattccggcc cgggtcaggc cgtcgactac
      361 ctcgtacgtg agcgtccccg agcgtgagat caagccgatc tcgccttcct taaagatgtg
      421 tcccggcatg atcccgacca gcgacttgcc cggcgagatc agccccgggc agttgggacc
      481 gatgatccgc atccccggcg tggttccgac gacctcgagc acgtcgtgca ccggcacgcc
      541 ttcggtaatg cagactgcca gcgtgattcc ggccgcatgc gcctcgtaca gcgcgtcggc
      601 cgcgaacgcc ggcgggacga agatgcaggt gtgggtcgcg ccggtggctt caaccgcctg
      661 tttgacggtg tcgaaaaccg gcagcccgtg cggtgttttc tggccgccct tgccgggcgt
      721 caccccgccg acgacgttcg tgccgtactt caccatccgg tcggtgtggt acgagccttc
      781 cgcaccggtg attccttgaa cgatgatctt gctgttcttg tcgaggaaaa tgctcacgct
      841 ttggctacct ccaccgcgcg ccgggcgcct tcgtccatcg tttcgaccgg cgtcatcccg
      901 gcctcggcga gaatccgccg cccttcttct tcgttcgtgc cggtgagccg aatgacgagc
      961 ggaatcttac gcgcggtcgt ctcgcgcatc gcgtcgacga tcccgcgcgc gacttcgtcg
     1021 ccgcgcgtga ttcccccgaa gatgttgatg aagaatacct tggctccggt gctgttcacg
     1081 acgaggtcgt aacagcggcg cacgcgctcc gcgttggcgc cgccgccgac gtcgagaaaa
     1141 ttcgcgacct cgccgccggc gtagctcacc gcatccatag tggccatcgc tagtcccgcg
     1201 ccgttggcca tcgtcccgac cgtcccaccg aaacggcgga agttgcgaat gcccagtccc
     1261 gccgcagcgg ccagccgttc gtcttcgtcg agcggcagcg cctgctgcca ctgcgcgaac
     1321 tcgggatggc ggaagagcgc gtcgtcgtcc agctcgacct tcgcgtcgga agcgtagacg
     1381 cggccgtcgc cggtcagcgc cagcggattg atttccacca gcttcgcgcc gtagcgaaag
     1441 aacaagtcgt agagcgcgcc gaggatcgcc ggaagttgct tgcggtagcc ggggtcgacc
     1501 tccgcttcga aggccagctc gcgtccgatg aagggcgagt atcccgcggc ggtgtctacg
     1561 tagtattttg cgatcgactc ggggtgcttt tcggatacct cctcgatgtc catcccgccg
     1621 aagcggctca ccatgatgac cggtttttta gcgctgcgat cgatcgcaat cgcgcagtag
     1681 gcttccttag caatcgggag cttctcttcg atcaatagcg acttgatcgg ctcgccggcc
     1741 gggttctgcc ggttgggcgg catcggcgtc gccaaaattt cctcggcaac cttatgtcct
     1801 tcgtcggcgg tattcgcgaa tttgatcttg ccggcttttc cccgcccgcc catcagcact
     1861 tgcgctttga cgacccactc gccggcgacg ctccccacgt actccgcgac gtcgtgcggc
     1921 gtgagcgcgt gccggctgcg cggcaccgga atgccggcac ggcgaaacag ctctttcccc
     1981 tgatactcca tgaggttcac ggtttgcgat ggctcctaac ggttgcgagt tagtcgagtt
     2041 ttccgagcag ggaacgcgcc acgatcaact gttggatttc cgacgtcccc tcgtagattt
     2101 cggtgatctt ggcgtcgcga tagtatcgct cgaccggaaa ttcggtcgta tagccgtacc
     2161 cgccgtggat ctgcaccgct tcggccgcgt gcttgcgggc ggcggtcgac gcgaacagct
     2221 tcgccttgct ggcctcgacc gcaaacggtt tgcccgcatc ggcgagcgcg gcggcgcggt
     2281 acaccagcag ccgcgcggcg tcgaggtcgg tggccatctg cgcgatcttg aacgaaacgc
     2341 cttcgaacgc cccgatcgga cgcccgaatg cagtgcgctc cttcgcgaat ttgaccgatt
     2401 cgtcgaggca ggccgcgaga atgccggtgg cttgagccgc gatgccgatc cgtcccgacg
     2461 tgagcgcggt catcgcgtag ccgaatcctg cgccttcggc gccgagcagc gcgccggccg
     2521 gaaccaccgc tccgtcgaac gcgacgtcgc acgtgttgct cgtgtggata cccagcttct
     2581 cggtgacctt ttcgaccgtc accccgggcg tcgcggcgtc gatcaagaac gcgctcacgc
     2641 ctttagcgcc ggtacggccg gtgcggaaca tccccatcat cacgccgcaa tagcttccgt
     2701 tggtgcacca ctgcttgcgg ccgtcgagcc ggtagccgcc gggcgtcgcc gtcgcggtcg
     2761 cacggatcgc ggccgcgtcc gagccggcgt cggattcggt cagcgcgaac ccggcgatca
     2821 cgtcctcgct cgcgagcttg ggcagccact cgcgcttttg ctcgtcgctg ccgcgtttga
     2881 gaatcgcggc gcagatcatg gcgtgcaccg aaacggtgac cgccgtgccc gcgtcgacgc
     2941 gcgccagctc ttcgatcgcc aacgcgtacg acacgtagcc ggaaccggca ccgccgtatt
     3001 cttccggcac ggcgatcccc atgattcccg cttcggtgag ctttccgtac agctcgcgcg
     3061 gaaacgcgtg ttcgcgatcc cagcgtgcga tcgacggcgc tatctcgcgc tgcgcgatct
     3121 ccgccgtcag cgccgcgatc gcgcgttgtt cgtcgg
//
LOCUS       DASUCJ010001255         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_915181, whole genome shotgun sequence.
ACCESSION   DASUCJ010001255 DASUCJ010000000
VERSION     DASUCJ010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915181"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="VFU90_14695"
     CDS             <1..>1032
                     /locus_tag="VFU90_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU5481089.1"
                     /translation="IMAVQSTVDASGPFALRASGSSLRDVFERWLAAREAGAAVAAAA
                     LSAERTVHVDAGWGGEAHRLVVRLASSGPFETRSHFLTLRRLGTQLARPAVPSALWCE
                     DDAGPLGAPFFVMTRVDGLTTARYETPYTFASFITEANPAERTQMQRATLEQLGRVHA
                     ATPSDFAFLDRRRPGETALSALVRKTVENYQVVSSRGLQAPVIERAFAWMREHWPADA
                     APVLCWGDARIDNAVYRDFGTVTLTGWRHATLGPRELDLGALVFHHRFADDLAHAVGR
                     PGLPEFLRPADVAATYADITGHHPESLDFYIVFAALRHAIEVIRSPLRIKAFEALAEA
                     MADERSREE"
BASE COUNT          151 a          354 c          356 g          171 t
ORIGIN      
        1 catcatggca gtgcaatcca cagtcgatgc ctccgggcct tttgctcttc gcgcgagcgg
       61 ctcatcgctg cgcgacgtct tcgagcgctg gctcgccgct cgagaggccg gcgccgcagt
      121 ggccgcggcc gcgctgtccg ccgagcggac ggtgcacgtg gacgccggtt ggggcgggga
      181 ggcgcaccgg ctcgtcgtgc gccttgcctc gagcggcccg ttcgagacgc gatcccattt
      241 cctgacgctg cgccggcttg gcacgcagct cgcccggccg gccgtgccgt ccgcgctgtg
      301 gtgcgaagac gacgccgggc cgctcggcgc tccgttcttc gtgatgaccc gcgtcgacgg
      361 gctgacgacg gcgaggtacg aaacgcccta cacgttcgcg tcgttcatca ccgaggcgaa
      421 cccggctgaa cgcacgcaga tgcagcgggc aaccttggag cagctgggcc gggtgcatgc
      481 ggcgacgccg tcggatttcg cgttcctcga ccggcgtcgt cccggggaga cagcgttgtc
      541 ggctttggtg cgcaagacgg ttgagaacta ccaggtggtc agctcccgag gattgcaggc
      601 gccggttatc gagcgcgcgt tcgcctggat gcgcgagcac tggccggcgg acgccgcgcc
      661 ggtgctgtgc tggggcgatg cacggatcga caatgcggtc taccgcgact tcggcacggt
      721 gacgttgacg ggatggcgac acgcgacgct cggcccgcga gaacttgacc tcggcgcgtt
      781 ggtatttcac caccgcttcg ccgacgacct cgctcatgcc gtcgggcggc cgggcctccc
      841 cgaattcctg cgccccgccg acgtggccgc cacatatgcg gacatcaccg gccaccatcc
      901 ggagagcctc gacttctaca tcgtcttcgc cgcactgcgg cacgcgatcg aggtgatacg
      961 aagcccgttg cgcatcaaag cgtttgaggc actcgccgaa gcaatggccg atgagcgctc
     1021 acgcgaagag ca
//
LOCUS       DASUCJ010001256         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1312354, whole genome shotgun sequence.
ACCESSION   DASUCJ010001256 DASUCJ010000000
VERSION     DASUCJ010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312354"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFU90_14700"
     CDS             <1..672
                     /locus_tag="VFU90_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5481090.1"
                     /translation="GVTLVGRVLAALRAAPSIGRIVAVAPPSMRDHPGLAAADELRPD
                     GVRITESLRSGLAGFSPDEDVLVAASDLPILTAPAIEDFVARARALEADVVYGCVERG
                     THLARYPQIPHTWARMQDGTFCGGGLISLKPRVLPKLERFVEQLGAARKRPWRLASLF
                     GWDVLLRYAAGRLTVAGAEARAGAILGAPVRALVSPYAETGVNVDRVTDVALAEELVA
                     AAGPV"
     gene            716..2122
                     /locus_tag="VFU90_14705"
     CDS             716..2122
                     /locus_tag="VFU90_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5481091.1"
                     /translation="MTLRELHDGANRVARGLARSGVGPGDRVAIYCENRLGFALTYLA
                     ALRLGAIAVPVNVLYRSADLAAVLDDARPAAIVCSPSSAPFAARAGAAKRTVDVAEIE
                     AWSRAPGDAGALPARPQPEDVALVVYTSGTTGRSKGAMLSHRNLAAIAAQLIAAWRWR
                     GDDVLLLTLPLFHVHGLIAGLTASLAAGSRVLLRERFDAGETLDRLAAGGVTMFFGVP
                     TMYVRMLEHAGERRIPPLRLYVSGSAALSAQTHDAFRRRFGAEILERYGATEFGFALG
                     NRYGGPRVAGSVGIPLPGVRVAVAAPGGGFAAPGEAGELLVNGPNVFAGYWDNPAATA
                     EAFFTDDAGTRWYRSGDIARYDPERDVYSIVGRIKEVVISGGFNVYPREVEDAIGAFP
                     GVEACAVIGNPDPIRGEVPIAFVEAVASLDVEALLAALRERLASFKIPKEIRVLDRLP
                     RNAMGKVNKPALKTLLQR"
BASE COUNT          304 a          738 c          760 g          375 t
ORIGIN      
        1 ggcgttacgc tggtcgggcg ggtgctcgcc gcgctgcgag cggcaccctc gatcggccgg
       61 atcgtagccg tcgcaccccc gtcgatgcga gaccatccgg gcctcgcggc ggccgacgag
      121 ctgcgccccg acggcgtccg gattaccgaa agcctgcgca gcggtctggc gggcttttcg
      181 ccggacgagg acgtgctggt ggcggcttcg gatctgccga tccttaccgc gccggcgatc
      241 gaagacttcg tcgcgcgcgc ccgggcgctc gaagcggacg tcgtctacgg ttgcgtcgag
      301 cgagggacgc acctcgcgcg ctatccgcaa attccgcata cttgggcgcg catgcaggac
      361 ggcacgtttt gcggcggcgg tctgatttcg ctcaaaccgc gcgtgctgcc gaagctcgaa
      421 cggttcgtcg agcaattggg ggcggcgcgt aagcgcccat ggcggctcgc atcgctcttc
      481 ggctgggacg tcttgcttcg ctatgcggcc gggcgcctaa ccgtggccgg cgcggaagcg
      541 cgcgccggcg cgattctcgg cgcgccggta cgcgcgctcg tttccccgta cgccgaaacc
      601 ggcgtgaacg tcgaccgcgt caccgacgtg gcgctggcgg aggagctggt cgctgctgct
      661 ggacctgttt gacggcgcgc tcgccgatcg cgcggatttt cccgcgttcg acgatctgac
      721 gctgcgcgag ctgcacgacg gtgccaaccg ggtggcgcgc ggtttagccc gcagcggcgt
      781 aggccccggc gatcgcgtcg caatctactg cgagaatcgg ctcggattcg cgcttacgta
      841 cctagccgcg ttgcgcctgg gtgcgatcgc cgtgccggtc aacgttctct atcgcagcgc
      901 ggatctggcc gccgtattgg acgacgcgcg accggccgca atcgtctgtt cgccgtcctc
      961 ggccccgttc gcagcgcggg ccggtgcggc gaagcgcacc gtcgacgtcg ccgaaatcga
     1021 agcctggtcg cgcgctcccg gcgacgcagg cgctttgccg gcgcgcccgc agcccgaaga
     1081 cgtcgcgctc gtcgtgtata ccagcggcac caccggacgc tcgaaaggcg ccatgctctc
     1141 gcaccgcaat ctggcggcaa ttgccgccca gttgatcgcg gcgtggcgat ggcgcggcga
     1201 cgacgtcttg ctgctgacgc tgccgctgtt tcacgttcac ggattgatcg ccgggttgac
     1261 cgcgtcgctg gcagccggtt cgcgcgtgct gctgcgggaa cgtttcgatg ccggcgaaac
     1321 gctcgaccgg ctcgccgccg gcggcgtcac gatgttcttc ggcgttccga cgatgtacgt
     1381 gcggatgctg gagcacgccg gcgagcgccg aattccgccg ctgcgcctgt acgtctcggg
     1441 ttcggccgct ctttcggcac agacgcacga tgcgttccgc cggcgtttcg gcgccgaaat
     1501 tctcgaacgc tacggagcca ccgagttcgg cttcgcgctg ggcaatcgtt acggcgggcc
     1561 gcgcgtcgcc ggcagcgtcg ggattccgct tccgggcgtt cgcgtcgcag tcgccgctcc
     1621 cggcggaggg ttcgctgcgc ccggtgaggc cggcgaactg ctggtgaacg gcccgaacgt
     1681 gtttgccgga tattgggata atccggcggc gaccgccgaa gcgttcttca ccgacgacgc
     1741 gggaacgcgc tggtatcgca gcggggatat cgcgcgctac gaccccgagc gcgacgtcta
     1801 ctcgatcgtc gggcggatca aggaagtcgt catcagcggc ggcttcaacg tctatccgcg
     1861 tgaagtcgaa gacgccatcg gagctttccc cggagtcgaa gcgtgcgcgg tgatcggcaa
     1921 cccggatccg atccgcggcg aagttccgat cgcgttcgtc gaagccgttg catcgctcga
     1981 cgtcgaggcg ctgctggccg cgctgcgcga gcgcctcgcc agcttcaaaa ttcccaagga
     2041 aatccgggtg ctcgatcgcc tgccgcgcaa cgccatggga aaagtgaaca aaccggcgct
     2101 gaaaacgctc ctgcaaaggt gacgggtaga cggccggccg cctcgcggcg ccttcgatta
     2161 cgcgggggat cgccagc
//
LOCUS       DASUCJ010001257         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_761990, whole genome shotgun sequence.
ACCESSION   DASUCJ010001257 DASUCJ010000000
VERSION     DASUCJ010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761990"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFU90_14710"
     CDS             complement(<1..245)
                     /locus_tag="VFU90_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481092.1"
                     /translation="MERSSELGSSLVETAVVLAIVAVLAGTALSAALGARHAAAGSPI
                     RDALQSAVRREMPIALDVLKYRGSAIVPASVPTTLPMP"
     gene            complement(191..>1284)
                     /locus_tag="VFU90_14715"
     CDS             complement(191..>1284)
                     /locus_tag="VFU90_14715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5481093.1"
                     /translation="AAWLQFLPETGFVGVHLFFFLSGFVITYPFVGAAAARRPAPSWG
                     HFAWRRFLKIVPSYALCIVVAYAIGYAQRQPGASTIPDVVTHVLFVHTWFPQRYGTIT
                     GVLWTLSVEVVFYCIFPIVWWCFKRRPWLTAAGMILVALAWRAEMAHCCYATLFPMYE
                     ENLPGYLDIFAFGALSAYVFTQIRARGISIGWCVGPLVALGGAALTIALLENLYGYRF
                     AEQWSSVWQIDRRPLLGAAFGLLALGFLVSPRWWQYLLDNPPLRFLAAISYNLYLYHQ
                     MLARELLALHVPPYLGDPHFDPQWQVRYTDVAFTATIAQATLVTYLYERPLLRIPPPR
                     LVTTPGGALRLTWSDRPNSEAVSSKPPSS"
BASE COUNT          285 a          399 c          421 g          179 t
ORIGIN      
        1 ggcatgggaa gcgtcgtcgg gacgctcgcc ggtacgatcg ccgagccgcg atatttcaag
       61 acgtccagcg cgatcggcat ctcgcgccga acggcggact gcagggcgtc gcgaatcggg
      121 ctgcccgccg cagcgtggcg cgctcccagc gccgcgctca gcgcggttcc ggcgaggacg
      181 gcgacaatcg ctaagacgac ggcggtttcg acgagactgc ttccgagttc ggacgatcgc
      241 tccacgtcag gcgaagtgcc ccgccgggcg tcgtaacgag tcgcggaggc ggtattcgca
      301 agagcgggcg ctcgtataga taggtcacga gcgtcgcctg agcgatcgtc gcggtaaacg
      361 ccacgtcggt ataccggacc tgccattgcg gatcgaagtg cgggtcgccc aaatagggcg
      421 ggacgtgcag cgccagcaac tcgcgtgcga gcatttggtg atagaggtaa aggttatacg
      481 agatcgcggc taagaaccgc agcggcggat tgtcaaggag atattgccac cagcgcgggc
      541 ttaccaaaaa tccgagcgcg aggagcccga acgccgctcc gaggagcggc ctgcgatcga
      601 tctgccaaac gctcgaccac tgctcggcga accggtagcc gtagaggttt tcgagcagcg
      661 cgatcgtcag cgccgcgccg cccagcgcga ccagcggtcc gacacaccag ccgatcgaaa
      721 tgccgcgcgc ccgaatttgc gtgaaaacgt acgcgctgag cgcgccgaat gcaaagatgt
      781 cgagataccc cggaaggttt tcttcgtaca tcggaaagag cgtcgcgtag cagcagtgcg
      841 ccatctccgc gcgccacgcc aaggcgacga ggatcattcc cgctgcagtc agccagggac
      901 ggcgtttgaa gcaccaccag acgatcggaa agatgcaata gaacactacc tcgaccgaaa
      961 gcgtccacag cacgccggtg atggtaccgt aacgttgcgg gaaccacgtg tgcacgaaga
     1021 gcacgtgggt gaccacgtcg ggaatggtcg aagcgccggg ttgccgttgc gcgtaaccaa
     1081 tggcgtacgc cacgacgatg cacagcgcgt aagaaggcac gatcttgaga aaacgacgcc
     1141 acgcaaagtg tccccacgac ggtgccggac gccgcgccgc cgccgcgccg acgaacgggt
     1201 acgtgatgac gaacccgctc aagaaaaaga acaagtgaac gccgacgaag cccgtttccg
     1261 gcaggaactg cagccacgcc gccg
//
LOCUS       DASUCJ010001258         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1083245, whole genome shotgun sequence.
ACCESSION   DASUCJ010001258 DASUCJ010000000
VERSION     DASUCJ010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083245"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFU90_14720"
     CDS             <1..286
                     /locus_tag="VFU90_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481094.1"
                     /translation="LGDVSSGAGVSIVRAPDVLPLRPESVRALAVDAAHATERFLASS
                     VAALRPSGRLLLPVASPLPDGVVERARDAHDRLGEKQDLTTLERAPRPLR"
     gene            complement(283..2139)
                     /locus_tag="VFU90_14725"
     CDS             complement(283..2139)
                     /locus_tag="VFU90_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5481095.1"
                     /translation="MTVTAESNTSTERDPRLSPTTLTRLFFDAVEGYDRPNALQVKAG
                     GAYQPISHRTLATRVRHAAFGLRALGIPDGSAVAILSENRPEWAIADYACLTARYVDV
                     PIYPTLPAEQVEYILEDSETVAIFISTSEQAQKIAAIRASLTNLKWVIAFDDLNGGGV
                     DFTLTALEAHGAASETPDASARYRADALAVSPDQVATIIYTSGTTGEPKGAMLTHGNL
                     ASNVAAVMKVIPITGEDASLSFLPLSHVLQRHFDYLMFATGVSIAYVESMDTVTVNMT
                     EIRPTLVVAVPRVYEKIYARVLDNALASGAIKKRIFFWARAVGERWADARLSGRTPNP
                     LLAAQYAVARRLVFSKLRERVGGRLRYFVSGGAPLAPVINKFFYAAGLVILEGYGLTE
                     TSPVIAVNTPDRYRIGTVGPPIAGIEVTIAPDGEIIVRGPNVMKGYFHKPEATREAID
                     ADGWFHTGDIGVLEDGFLRITDRKKDIIVTAGGKNIAPQPIENHIRMNKYVSQAVMIG
                     DQRRFPIVLVVPNFEQLERWAKLKNLLYADRRQLLAQPMVKAKMEKEVLGKLAGLAHF
                     ETPKKMALLDRDFTVDSGELTPTLKVRRRVIDKKYKAVIDQLYAGDEAAHRA"
     gene            complement(2181..2960)
                     /locus_tag="VFU90_14730"
     CDS             complement(2181..2960)
                     /locus_tag="VFU90_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481096.1"
                     /translation="MMRPSLRAASIALSIAAVAAACNEVGTNANAPVAIQLDAPASPS
                     VLLGDTLRDSLGQVAPLHVVVFNFKNDSIPGIPVSFLAVDSTHSVTVDPALGIVVGHA
                     LIARVLVVATAAGLQSIADTIAVVDTPSVMLAEDSSRDSVDYSFANGLRDTTITLRVR
                     LLHLPDAVPVPTYAVHYTIVHPSPYDNTDSTNAQLVKIGGTVGQVVDTTDANGSTNLG
                     LRITPFTHAFNDSIVVQVSASAPGGLPAGSPITYVRRINIH"
     gene            complement(2957..3724)
                     /locus_tag="VFU90_14735"
     CDS             complement(2957..3724)
                     /locus_tag="VFU90_14735"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5481097.1"
                     /translation="MGTVLAVVSQKGGVGKTTTAVNLAAAFARRGMKTLLVDVDPQGA
                     VRYGVGLRADHVTVGISDYLSGEKTLRDIILPTALPWLRVILAGTVSAATNHVAYQHR
                     MEESTLLPDLLAMARQRTDVVVVDTPPGLGPTVERVLSASQHVIVPLQAEPLALQTTP
                     QILRGIQDVVMTNDELTLDGLVLTMYDETSETSVRVSDYVRRHLPRNMVFDVVIPRST
                     AAADAFAAGQPVVLRSPADAAAQAYVNLATELASRFE"
     gene            complement(3728..4183)
                     /locus_tag="VFU90_14740"
     CDS             complement(3728..4183)
                     /locus_tag="VFU90_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5481098.1"
                     /translation="MSWWSRLTGGGTAHTPRPQRLDYLSEALALERQGDYDAALTSYR
                     LALRDQPNDQRILQNMAIAYSRTGRLDDAIRCYRRALEIDPQLSGAHYGLAYLLLKRG
                     DGQGAQRHLTAFLEHPPSTVEAERWVQHARQTIEQIKTGGGAGSVPGTE"
BASE COUNT          708 a         1492 c         1484 g          775 t
ORIGIN      
        1 gttaggcgat gtctccagcg gcgccggcgt gtcgatcgtg cgcgcgcccg acgtgctgcc
       61 gctccgtccc gaatcggtgc gcgcgctggc ggtcgatgcg gcgcacgcga ccgaacgctt
      121 tctcgcgtcc agcgtcgccg cgctcaggcc ctccggccgc ctgctgctgc ccgtcgcgag
      181 cccgctgccc gacggcgtcg tcgagcgcgc gcgcgatgcc cacgaccggc tgggcgagaa
      241 gcaggacctc accaccctcg agcgggcgcc gcggccactg cgttaggctc ggtgcgccgc
      301 ctcgtcgccg gcatagagct ggtcgatcac cgccttgtac ttcttgtcga tcacccggcg
      361 gcgcaccttg agcgtcggcg tcagctcgcc ggaatcgacg gtgaagtcgc ggtccaacaa
      421 cgccatcttc ttcggcgtct cgaagtgcgc cagccccgcg agcttgccca acacttcttt
      481 ttccatcttc gccttgacca tcggttgggc gaggagctgc ctgcggtccg cgtagagcag
      541 gttcttgagc ttcgcccagc gctcgagctg ctcgaagttg ggcaccacga gcacgatcgg
      601 aaatctccgt tggtcgccga tcatcaccgc ctgcgacacg tacttgttca tccggatgtg
      661 gttctcgatc ggctgcggcg cgatgttctt tcccccagcg gtcacgatga tgtccttctt
      721 gcgatccgtg atgcgcagga agccgtcttc cagcacgccg atatcgccgg tgtggaacca
      781 gccatcggca tcgatcgcct cgcgcgtggc ctcgggcttg tggaaatagc ccttcatcac
      841 gttgggtccg cgcacgatga tctcgccgtc cggcgcgatc gtcacctcga tgccggcgat
      901 cggcggcccc accgtgccga tgcgatagcg gtccggcgtg ttcacggcga tcaccggcga
      961 tgtttcagta aggccgtagc cttccagaat caccaacccg gcggcataga agaacttgtt
     1021 gattaccggc gccagcggag cgccgcccga cacgaaatac cgaaggcgcc cgcccacgcg
     1081 ctcgcgcagt ttcgagaaca cgaggcgccg cgcgaccgca tactgtgccg cgagcagcgg
     1141 attgggcgta cgaccggaca ggcgagcgtc tgcccaccgt tcgccgacgg cgcgcgccca
     1201 gaaaaaaatg cgcttcttga tcgcgccgct cgccagcgcg ttatccagca cccgcgcata
     1261 gatcttctcg tacacgcgcg ggaccgccac caccagcgtt gggcggattt ccgtcatgtt
     1321 cacggtcacg gtgtccatcg actccacata cgcgatcgac acgccggtcg cgaacatgag
     1381 atagtcgaag tgccgctgga gcacgtgcga aagcggaagg aagctgagcg atgcgtcttc
     1441 tcccgttatc gggatgactt tcatcaccgc cgccacgttg gacgcgaggt tcccgtgcgt
     1501 gagcatcgct cccttgggct caccggtcgt gccggacgta taaatgatcg tcgccacctg
     1561 gtccggtgag actgccaacg cgtctgcccg atagcgcgcc gaggcgtccg gcgtctcgct
     1621 cgctgcaccg tgcgcttcga gtgcggtgag agtgaaatcc acgccgccgc cattcaggtc
     1681 gtcgaacgca atcacccact tgaggttggt cagcgatgcg cgaatggccg cgatcttctg
     1741 cgcttgctcg ctcgtcgaga tgaaaatcgc gacggtctcg gagtcctcga ggatgtactc
     1801 gacctgctcg gccggcaggg tcgggtagat cgggacgtcg acgtaccgcg cggtgagaca
     1861 ggcgtagtcg gcgatcgccc actccgggcg attctcggac aggatggcaa cggccgagcc
     1921 atccggaatg ccgagcgcgc ggagcccgaa cgcggcgtgg cgaacccgcg ttgccagcgt
     1981 gcggtgcgag atcggctgat acgcgccgcc ggccttcacc tgcagtgcgt tcggccgatc
     2041 gtacccttcg acggcgtcga agaagaggcg cgtgagcgtc gtcggcgaca gtcgcggatc
     2101 gcgttccgtc gacgtgttcg actcggcagt caccgtcatg gaggcctcgc tcgcggcggc
     2161 gcgctccgac cgcgctgcgc tcagtggatg ttgatgcgcc taacgtatgt gatcggactg
     2221 cccgccggta gcccgccggg cgccgatgcg ctcacctgta cgacgatcga gtcgttgaac
     2281 gcgtgcgtga acggcgtgat gcgcaggccg aggttggtgc tgccgttcgc gtccgtcgtg
     2341 tccaccacct gtccgaccgt tcccccgatt ttcacgagct gcgcattggt cgaatccgtg
     2401 ttgtcgtagg gcgacgggtg cacgatcgtg tagtgaaccg cgtacgtggg cacgggcacc
     2461 gcgtccggca ggtgcagcag gcgcacgcgc agcgtgatgg tcgtatcgcg cagtccgttg
     2521 gcaaaactgt agtcgacgct gtcccgcgat gaatcttcgg ccagcatcac ggatggcgtg
     2581 tcgaccacgg cgatcgtgtc ggcaatcgac tgcaatccgg cggccgtcgc caccacgagc
     2641 acgcgcgcga tgagcgcgtg tccgaccacg atgcctaacg ctgggtcgac ggtcaccgaa
     2701 tgcgtcgaat cgacggccag gaacgacacc gggatgcccg ggatgctgtc gttcttgaag
     2761 ttgaagacca cgacgtgcag cggcgccacc tgccccaggc tgtcgcgcag cgtatcgccg
     2821 aggaggacgg acggcgacgc gggcgcatcg agctggatcg cgaccggcgc gttggcgttg
     2881 gtgccgacct cgttgcacgc cgcggccacc gcggcgatcg acagcgcgat cgacgccgcc
     2941 cggagggagg gccgcatcac tcgaaccgcg atgcgagctc ggtggcgagg ttcacgtacg
     3001 cctgcgccgc cgcgtcggcc ggcgagcgca ataccaccgg ctggcccgcc gcgaacgcat
     3061 cggccgccgc cgtgctccgc ggaatcacca cgtcgaacac catgttgcgc ggcaggtggc
     3121 gccgcacgta gtccgacacg cgcacgctgg tttcgctcgt ttcgtcgtac atcgtgagca
     3181 cgagtccgtc gagcgtcagc tcgtcgttag tcatcacgac gtcctggatg ccgcgcagaa
     3241 tctgcggcgt cgtctggagc gccagcggct ccgcctgcag cggcacgatc acgtgctgcg
     3301 aggccgagag cacccgctcg accgtcgggc ccagtccggg cggcgtgtcg accacgacga
     3361 cgtccgtgcg ctgacgcgcc atggccaaca agtcgggcag caatgtggac tcctccatcc
     3421 ggtgctggta tgcgacgtga ttcgtggccg cactgacggt cccggccaga atcacgcgaa
     3481 gccatggtaa cgccgtcggc aaaatgatgt cgcgcagcgt cttctctccc gatagataat
     3541 cggagatgcc gacggtgaca tggtccgcgc gcagccccac gccgtagcgc acggcgccct
     3601 ggggatccac gtcgacgagg agcgttttca tgccgcggcg agcgaacgcg gcggcgagat
     3661 tcactgcggt ggtcgtctta ccgacaccgc ctttctggct cacgacggcg aggacggttc
     3721 ccactcgcta ttccgtgccg gggacggagc ccgccccgcc gccggtcttg atctgctcga
     3781 tggtctgccg ggcatgctgg acccaccgtt ccgcttcgac tgtggacggc ggatgctcca
     3841 gaaacgccgt caggtggcgc tgggcgcctt ggccgtcgcc gcgcttgagc agcaggtacg
     3901 cgaggccata atgcgcgccg ctcaactgcg ggtcgatttc gagtgcccgg cgatagcagc
     3961 ggatggcgtc gtccagtcgt ccggttcgcg aatatgcgat cgccatgttc tggaggatgc
     4021 gctgatcgtt gggctgatcg cgcagtgcga gccggtacga cgtgagcgcg gcgtcgtagt
     4081 caccctggcg ctccagcgcc agcgcttcgc tcaagtagtc gagacgttgt gggcgcggcg
     4141 tgtgcgccgt tccaccaccc gtcaagcggc tccaccagga catgcgatgc gtctagagtc
     4201 gagaacttgg gtgggaaggc gcaccacggc ggcgcggtgc aggaatgcgc actcggcgta
     4261 cctgtcgagg gcaaacttat gcaggcccac gcgaggcggc aaccgcgctg tcggccgcga
     4321 gcgtgcgacg gtagctttcg cgctgagtcg aagtcggccg ctaacctgga ggacacgtgg
     4381 cgccacgacg taaacccgcg gaccgaacgc gcggcgtctt cgaagtggac tggccgctct
     4441 tcggcgagct gtctcgcgc
//
LOCUS       DASUCJ010001259         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_501875, whole genome shotgun sequence.
ACCESSION   DASUCJ010001259 DASUCJ010000000
VERSION     DASUCJ010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501875"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFU90_14745"
     CDS             <1..490
                     /locus_tag="VFU90_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481099.1"
                     /translation="VTRDYLAYHEEKEAHAKGAISLERATYSGWYAIQRLELAPEGSI
                     RCGDPLVVSGEAFSPDGRTPVIMVGLVRADGTAVYGVSTDMDDSGPSQIGPNHFAFTL
                     EFPALDLLPGKYHVRAHAADPEGVRVFDYIERELVVAGETRELGLVRLAHRWNPGTDR
                     RA"
     gene            531..1463
                     /locus_tag="VFU90_14750"
     CDS             531..1463
                     /locus_tag="VFU90_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481100.1"
                     /translation="MSAFRTHWRWPLVTATLPWPLRFRALSRIARNDRVAFPEEAAAY
                     AEARRFGFACLPENPGTIAGEAEWCRAWRLVQLVDHDDLFCTLRHRRSTIARSLEVIG
                     EWPAKGPFLALTYHWGAGLLALAHLAQNGRRARFVSARFDQRSLGDDAWRWRYAKLRG
                     RATERLCAGSIIYTGDATDRIVAALKQGDCVVGLCDALIDEGRSRVDAPFGDFRLSMP
                     RGLVRLAVASSTTVVPFAAGVDRATGRRRLEIGDARSYVDESELAARLSERLFRLVQR
                     DPAAWHMWPHASRILADDRRARPAAPADPGQRQT"
     gene            complement(1471..2229)
                     /locus_tag="VFU90_14755"
     CDS             complement(1471..2229)
                     /locus_tag="VFU90_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HEU5481101.1"
                     /translation="MDQSDVLNAIRVRRWFYEFDLPDGTRTASYLPPGVEKIHSTRLD
                     MLWRGLQPVVRDEWPQLSVIDVACHQGYFATQLARKGCHDVLAIDARDEHVADTDLIR
                     KALGLTNLRTLRADINALDAARIGTFDITLMLGLLYHVENPVGVLRLARALTRRVCVI
                     ETQVVPNMTGVVDWGAYTFQRPMVGSVAIIDETAETHAPEASTTGICLCPSYEALVWL
                     LHKVGFATVDRVEPPADAYEQHASGKRVMVVARV"
     gene            complement(2288..2548)
                     /locus_tag="VFU90_14760"
     CDS             complement(2288..2548)
                     /locus_tag="VFU90_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5481102.1"
                     /translation="MKDFRPAKKRADVSVGESVRIVRELQGLSQNQLAELSGIPQATL
                     SAIERDRVNLRVERAKVLARALRCQPAVLVFPGWDQSSESAA"
     gene            complement(2550..2831)
                     /locus_tag="VFU90_14765"
     CDS             complement(2550..2831)
                     /locus_tag="VFU90_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481103.1"
                     /translation="MWRILEHRRVDKQCVNAPVEILRRYEKWKDIAAVSGPPGLRQIA
                     GFHDEAVVGRWKGYRSSRLGLQYRLIYRVLADETTFQLVSLTAHDYRRT"
BASE COUNT          491 a          961 c          975 g          562 t
ORIGIN      
        1 cgtgacgcgc gactacctcg cctaccacga ggagaaggaa gcgcacgcga agggggcgat
       61 ttcgctcgag cgcgcgacgt attcggggtg gtacgcgatt cagaggctcg agctcgcacc
      121 cgagggctcg atccgttgcg gcgatccgct cgtggtgtcc ggtgaagcct tttcgccgga
      181 cgggcgaacg ccggtgatca tggtcggact ggtacgggcg gacggaactg cggtctatgg
      241 cgtgtcgacc gacatggacg attccggtcc gtcgcagatc ggccccaacc acttcgcgtt
      301 caccctcgag ttccccgctc tcgacctgct gccgggcaag tatcacgtcc gcgctcatgc
      361 agcggacccc gagggtgttc gcgtcttcga ctacatcgag cgcgagctcg tggtcgcggg
      421 cgagacgcgc gagctcggcc tcgtgcgatt ggcgcatcga tggaatccgg gcacggatcg
      481 gcgggcctga tgttgtgcgg ccggaatacg tgcgcgatct ttgggcgagc gtgagcgcgt
      541 tccggacgca ctggcgctgg ccgctcgtga cggcgacgct tccgtggccg ctgcgatttc
      601 gcgcgttgtc gcggatcgca cgcaacgatc gcgtcgcatt ccccgaggag gccgctgcgt
      661 acgccgaagc gcgccgattt ggcttcgctt gcttacccga gaatcccgga acgatcgccg
      721 gcgaagcgga gtggtgccgc gcctggcgtc tcgtgcaact cgtcgatcat gacgacctct
      781 tctgcacgct ccggcatcgc cggtcgacga ttgcgaggtc tctcgaagtc atcggcgaat
      841 ggccggcgaa gggcccgttc ctcgcgctca cttatcactg gggcgcaggg ctgcttgcgc
      901 tcgcgcatct cgcgcagaac ggtcgacgag cgagattcgt ctccgctcga ttcgaccagc
      961 gttcgctcgg cgacgacgcg tggcgctggc gctacgcgaa gctgcgcggc cgggccaccg
     1021 agcgactttg cgccggctcg atcatctata cgggcgacgc caccgaccgg atcgtggctg
     1081 cattgaagca gggtgattgc gtcgtgggat tgtgcgacgc gttgatcgac gaagggcgat
     1141 cacgcgtcga cgctccattc ggcgactttc gcctctcgat gccaagaggc ctggtgcggc
     1201 tcgcggtcgc gtcgagcacg acggtcgttc ccttcgcggc gggagtcgat cgcgcaacgg
     1261 gtcgccgccg tctcgagatc ggcgacgcac gctcgtatgt cgacgagagt gagctcgctg
     1321 cgaggttgtc cgagcgcctg tttcggttgg ttcagcgtga tcccgcggca tggcacatgt
     1381 ggccgcacgc gagtcggatt ctcgcggacg accgtcgtgc tcgaccggcg gcgcccgcgg
     1441 atccggggca acgtcagact tgaggtaccg tcaaacgcgc gcgacgacca tcacccgctt
     1501 gccactcgcg tgctgctcgt aagcgtcggc gggcggctcg acgcggtcca ccgttgcgaa
     1561 tccgaccttg tgcagcagcc agaccagcgc ttcgtaactc gggcacagac atattcccgt
     1621 cgtcgatgct tcgggcgcgt gcgtttccgc cgtctcgtcg atgatggcga cactccccac
     1681 catcgggcgc tgaaacgtgt acgcacccca atcgacgacg cccgtcatgt tcgggacgac
     1741 ctgcgtttcg atgacgcaga ccctgcgtgt gagtgcccgc gcgaggcgca gtacgccgac
     1801 cggattctcg acgtgataca gaaggccgag catcagcgtg atgtcgaacg ttccgatccg
     1861 cgccgcgtcc agagcattga tgtccgcccg cagtgttcgc aggttcgtga ggccaagagc
     1921 ctttcgaatc agatcggtgt ccgcgacatg ctcgtctcgc gcgtcgatcg cgagcacgtc
     1981 gtggcatccc ttgcgcgcga gctgcgttgc gaagtagccc tgatggcagg cgacgtcgat
     2041 gacggagagc tgcggccact cgtcacgaac gacgggttgc agaccacgcc agagcatgtc
     2101 gaggcgcgtc gagtgaatct tctccacgcc cggcggcagg taggacgctg tccgcgtgcc
     2161 atccgggagg tcgaactcgt agaaccagcg gcgcacgcga atcgcgttca gaacgtctga
     2221 ttggtccatg gcatgactta cttcgttgcc cgaatcggcg attccggcga gtggggttcg
     2281 cgtatgctca cgccgcactt tccgaggact gatcccagcc ggggaagacc agtacggcgg
     2341 gctggcagcg aagcgcgcgt gcaagcactt tggcgcgctc gactcgcagg ttgacgcggt
     2401 cgcgctcgat cgccgacagc gtcgcctgag ggatgccgct caattcggca agctgattct
     2461 ggctaaggcc ctggagctct cgcacgattc gtaccgattc gccgaccgaa acgtccgcac
     2521 gcttcttcgc aggacggaag tccttcatgt cacgttctcc gatagtcgtg cgccgtgagg
     2581 gaaaccagct ggaaggtcgt ctcgtcggcc aggacccgat agatcaaccg atactggagc
     2641 ccaagtcggg acgagcgata acccttccat cggcccacga ctgcttcatc atggaatcca
     2701 gcgatttgcc ggagtccggg aggaccggaa acggccgcaa tgtccttcca cttctcatag
     2761 cgcctgagaa tctcgaccgg cgcgttgacg cattgcttgt cgacgcgtcg atgctccagg
     2821 atgcgccaca tgtcgaatca tatctatacc atatttagta tgtcaacggc gtcggatagg
     2881 tcatcgcgga ccgagtcccc gcggtagaat tcgccgatcc tggctgtcct ttccgatgcg
     2941 cgacgcgacc cctttcaccg gcgagcgcta cctccccggc gttcccggc
//
LOCUS       DASUCJ010001260         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_487040, whole genome shotgun sequence.
ACCESSION   DASUCJ010001260 DASUCJ010000000
VERSION     DASUCJ010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487040"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFU90_14770"
     CDS             <1..976
                     /locus_tag="VFU90_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU5481104.1"
                     /translation="PGIFYAKDAFEGLALVDALTGEPARRADLLERTKREALAQRDRG
                     HRAPAAAATAPSAVKSDVAEVPRPPFWGARTLERIDWNELWTCFDRRSLFRLSWGAAN
                     TKGEAYDRLLRDDFEPRLARYQQRAVRDGWLLPRVAYGYFPAAGAGNAVILYDPQDPA
                     REVARFDFPRQAGGEHLSLADYLREPEGGAGADVVALQVVTVGPQPAQRVEALQAAGE
                     YGESYFLHGFSVQAAEALAEYTHRHVRSELRLEEGRGKRYSWGYGACPDLAQQAIAFE
                     LLGAGERIGVALTEAFQIVPEQSTAAIVIHHPRASYFNAAATRELRVS"
     gene            992..>1608
                     /locus_tag="VFU90_14775"
     CDS             992..>1608
                     /locus_tag="VFU90_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5481105.1"
                     /translation="MTVHTALFAGLFAVAAILIVALVRAFPRHSPVMPRPVAGAIATA
                     IGFVTNFFDTLGIGSFATTTSLFKFLRFVPDEQIPGTLNVGHALPTVVEAAIFIGIIA
                     VDPRTLIALIFAAVVGAWLGAGLVARLPRRFVQIGMGVALLAAAALFIASNLKGSQFG
                     LGGTALGLDGTRLLIGIAVSFCLGALMTIGIGLYAPCMIMVGLLG"
BASE COUNT          238 a          512 c          563 g          295 t
ORIGIN      
        1 tccgggaatt ttttacgcga aggacgcgtt cgaaggcctc gcgctcgtgg atgcgctcac
       61 cggggagccg gcgcgacggg ccgacttgct ggagcgtacc aaacgcgaag cgctcgccca
      121 gcgcgaccgc ggccaccgcg cgccggcggc cgccgcgacc gcgccgtcgg cggtgaaaag
      181 cgacgtcgcg gaggttcccc ggccgccgtt ttggggagcg cgcaccctcg agcggatcga
      241 ttggaacgag ttgtggacgt gcttcgaccg gcgcagcctc tttcgattgt cgtggggcgc
      301 ggccaacacc aaaggcgaag cctacgaccg tttgctgcgc gacgatttcg aaccgcggct
      361 ggcgcgttat cagcagcgag ccgtgcgcga cggctggctg ctcccgcggg tcgcgtacgg
      421 ctatttcccg gcggccggag cgggcaatgc ggtcatcctc tacgacccgc aagacccggc
      481 gcgggaagtt gcgcgcttcg actttccgcg tcaggccggc ggcgagcatc tcagcctcgc
      541 cgactatctg cgcgagcccg aaggcggtgc gggtgccgac gtggtcgcgc tgcaggtggt
      601 cacggtcgga ccgcaacccg cgcagcgcgt cgaagcgctg caggcggcgg gcgaatacgg
      661 cgagtcgtac tttcttcacg ggttttcggt gcaggccgcc gaggcgctgg ccgaatatac
      721 gcatcgacac gtccgcagcg aactgcgctt agaagaagga cgcggcaagc ggtattcgtg
      781 gggttacggc gcgtgtccgg atctcgcaca gcaagcgatt gcattcgagc tgctcggggc
      841 cggcgagcgg atcggcgtgg cgctcaccga ggccttccaa atcgtgccgg agcagtcgac
      901 ggccgcgatc gtgatccatc atccgcgcgc gagctacttc aacgccgcgg cgacccggga
      961 actccgcgtc tcgtgagcca atagcgaagc gatgacggtc cacaccgctc tgttcgccgg
     1021 tctgttcgcc gtggcggcga tcctgatcgt cgcgttggtg cgcgcgtttc cgcgtcacag
     1081 cccggttatg ccccggccgg ttgcgggagc catcgcgacg gccatcggat tcgtcacgaa
     1141 cttcttcgat acgctcggca tcggttcgtt cgcgaccacg acgtcgctct tcaaattctt
     1201 gcgattcgtt cccgacgagc aaataccggg cacgctcaac gtcggacacg cgcttccgac
     1261 ggtggtcgaa gcggcgatct tcattgggat cattgcggtc gatccccgaa cgctgatcgc
     1321 gttgattttc gcggccgtcg tgggcgcttg gctgggagcc ggcctggtcg cgcgtttgcc
     1381 gcggcgcttc gttcagatcg gcatgggggt cgcgctgttg gcggccgccg cgctcttcat
     1441 cgcgagcaac ctcaaaggga gccagttcgg gctcgggggt acggcgctgg ggctggatgg
     1501 aacccgcctg ttgatcggga tcgccgtcag tttttgcttg ggagcgttga tgacgatcgg
     1561 aatcggcctg tacgcgccct gcatgatcat ggtgggcctg ctggggat
//
LOCUS       DASUCJ010001261         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1495938, whole genome shotgun sequence.
ACCESSION   DASUCJ010001261 DASUCJ010000000
VERSION     DASUCJ010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495938"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1676
                     /locus_tag="VFU90_14780"
     CDS             <1..>1676
                     /locus_tag="VFU90_14780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481106.1"
                     /translation="FTLTAVLTLAVGLGFNAAIFTTIYALTFRRLPVANAGALANVYQ
                     THHGTYRRLVNGMPSMVSYREYLEYRSLIDSSRGRAAAVVSAAVYRESEFSVTWAHSL
                     TAHGQYVSCNYFRVLDARMAEGRPFVDADCAHAGSAPIAILSHAFWSRELASDSSVLG
                     ATIRVGNALVTVVGVAAAGFAGPALQPSDLWLPVTMEPVLDAKGSLLPEDGSWLSMIV
                     RLAPGATRAAARAELTVAAARRDRAYPGRSTTVIVRPAAFFNAPEEVAATGLFSILTG
                     LLGLLVVGMVCANIMNLLLAHGFARRREIGTRIAIGASRGRIIEQLVIENVVLALAGG
                     GVGLASAYALPALAARLPAVSTLQFNLSPDLRVAAFTFALSIAAALTFGLVPALQTTN
                     VDIASQARDGGVRGRRASPARWRSAVIQVQLAGSALMLSVAAILSTAVRHGTEVDPGY
                     ITRDVVAFDLNLAMLGYNEARTADVYGRVIRRLGATPGVAGVALASPLPLLARSTERV
                     TTFDSSGQARTLDGVGQVAASGSYLATMQIRLIAGRSYRDGDTPSGSASG"
BASE COUNT          244 a          544 c          584 g          304 t
ORIGIN      
        1 cgttcacgtt gacggcggtg ctcacgctgg cggtcggcct tggattcaac gccgcgatct
       61 tcacgacgat ctacgcgctc accttccgcc ggctaccggt ggccaatgcc ggcgcactcg
      121 caaatgtcta ccagacgcac cacggcacct accgacggtt ggtcaacggc atgccgagta
      181 tggtgtcgta ccgggagtac ctggaatacc gttcgttgat cgactcttcg cggggccgcg
      241 ccgcggcggt ggtgagcgcg gcggtctacc gggagtccga gttctccgtg acgtgggcgc
      301 actccctgac ggcgcacggc cagtatgtgt cgtgcaacta cttccgggtg ctggacgcgc
      361 gcatggcgga ggggcggcca ttcgtcgacg ccgactgcgc gcatgcgggc tcggcgccca
      421 tcgccattct gagtcatgcg ttctggtccc gagaactcgc gagcgactcg tcggtgctcg
      481 gggcgacgat ccgcgtgggg aacgcattgg tcaccgttgt tggcgtggct gcggcgggct
      541 ttgccgggcc ggcactccag ccgagcgatc tctggctgcc cgtgacgatg gagccggtcc
      601 ttgacgcgaa ggggagctta ctgcctgagg atgggagctg gctgtcgatg atcgtgcggc
      661 ttgccccggg cgccacgcgt gcggcagcgc gtgcggaact cacggtggcc gcggcccggc
      721 gcgaccgggc ctacccgggg cgaagcacga cggtgatcgt acgaccggcg gcattcttca
      781 atgcgccgga ggaggtggcc gcgacgggac tgttcagcat cctaacgggg ctcctggggt
      841 tgctcgtcgt cggaatggtt tgcgcgaaca tcatgaacct gttgctcgcg cacggattcg
      901 cacgccgccg cgaaatcggc acgaggatcg ccatcggcgc ctcgcgcgga cggatcatcg
      961 agcagctggt gatcgaaaac gtcgtgcttg cgctcgctgg cggcggggtt ggactcgcga
     1021 gcgcgtacgc cctgccggcg ctcgccgcgc ggctcccggc cgtatcgacg ctgcagttca
     1081 atctctcacc ggatcttcgc gtggctgcgt tcacgttcgc actctcgatt gcggcagcgc
     1141 tgacattcgg gttggttccg gccctccaga cgacgaacgt cgatatcgcg tcgcaagcga
     1201 gagacggcgg cgtgcgaggt cgtcgtgcat ctcccgcgcg ctggcggagc gcggtgatcc
     1261 aggttcagct ggcgggtagc gcgctgatgt tgagcgtcgc ggcgattctc tcgactgcgg
     1321 tgcgccacgg cacagaagtc gacccggggt acatcacgcg agacgtagtc gcgttcgacc
     1381 tcaacctcgc catgcttggt tacaacgagg cgcggaccgc tgacgtctac ggccgagtga
     1441 tccgtcgcct cggggccacc cccggagtgg cgggcgtcgc ccttgcttca ccattgccgc
     1501 tcttggcgcg gagcaccgag cgcgtgacga cgttcgactc atccggtcaa gcacgcacgc
     1561 ttgatggagt agggcaggtc gccgcctccg gcagttacct ggctaccatg cagatccgac
     1621 tgatcgccgg acgctcgtac cgagacggcg acaccccatc cggttctgcg agcggg
//
LOCUS       DASUCJ010001262         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_731734, whole genome shotgun sequence.
ACCESSION   DASUCJ010001262 DASUCJ010000000
VERSION     DASUCJ010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731734"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFU90_14785"
     CDS             <1..727
                     /locus_tag="VFU90_14785"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481107.1"
                     /translation="GGCDSPPHAQVDTIDPSGYEGNPVYPCFERPTLGDLLDAKSVSW
                     RYYQESGGPGLWHPYDAIRHIRDGNDYLKVVWPSDVVLSQIKSGQLPQVSWVAPSGAN
                     SDHAGTRSNGGPAWVSSIVNAVGQSQYWNDCAIFVVWDDPGGWYDHVAPQTFNSYELG
                     FRVPLIVISPYAKSGYVSHTQYEFGSILKFVEDAFQTGSLKTTDQRANSLNDTFNFNQ
                     QPRRFVRIQAPPVRFGGSAPDSD"
     gene            complement(719..>1030)
                     /locus_tag="VFU90_14790"
     CDS             complement(719..>1030)
                     /locus_tag="VFU90_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5481108.1"
                     /translation="CLVHGDLYARHVLVGDDGAASGIIDWGDAHLGDPAVDLAIAFSI
                     FSNGARAAFAQAYGGIDARTWELARYRAAYHSAMLAHYGHRIGDARIAEAGLSGLARS
                     V"
BASE COUNT          191 a          343 c          327 g          169 t
ORIGIN      
        1 cggcggctgc gattcgccgc cgcacgccca ggtggacacg atcgacccga gcggttacga
       61 aggcaatccg gtgtacccgt gtttcgagcg cccgacgctt ggcgatttgc tcgatgccaa
      121 gagcgtgagc tggcgctact atcaggagtc gggcggcccg ggtttgtggc acccatacga
      181 cgccattcgt cacatccgcg acggcaacga ttacctcaaa gtcgtttggc cgtcggacgt
      241 cgtgctctcg cagatcaaga gcgggcaact gccgcaagta tcgtgggttg caccctcggg
      301 cgccaacagc gatcacgccg ggacgcgcag taacggcggt cccgcgtggg tgtcctcgat
      361 cgtcaacgcg gtagggcaga gccagtattg gaacgattgc gcgatcttcg tcgtttggga
      421 cgaccccggc gggtggtacg accacgtcgc gccgcagacg ttcaacagct acgagctcgg
      481 cttccgcgta ccgctgatcg ttatctcgcc gtacgccaaa tcgggctacg tatcgcacac
      541 ccagtacgag ttcggcagca tcctgaagtt cgtcgaggac gcgtttcaga ccggatcgct
      601 caagaccacc gaccagcggg cgaactcgct caacgacacg ttcaatttca accagcagcc
      661 gcggcggttc gtgcgcatcc aagcgccgcc cgtgcggttc ggcgggagcg cgcccgactc
      721 agactgagcg cgcgaggccg gagagacccg cttcggcaat tcgtgcgtcg ccgatccgat
      781 ggccgtagtg cgccaacatc gcggagtggt atgccgcccg atagcgggcc aactcccacg
      841 tccgcgcgtc gattccgccg tacgcctgcg cgaacgccgc gcgcgcaccg tttgaaaaga
      901 tcgaaaacgc gatcgccagg tcgaccgccg gatcgccgag atgcgcgtcg ccccagtcga
      961 taattccgga ggcggcaccg tcgtcgccga ccaaaacgtg gcgcgcgtag aggtcgccgt
     1021 gcaccaagca
//
LOCUS       DASUCJ010001263         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1343213, whole genome shotgun sequence.
ACCESSION   DASUCJ010001263 DASUCJ010000000
VERSION     DASUCJ010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343213"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..450)
                     /locus_tag="VFU90_14795"
     CDS             complement(157..450)
                     /locus_tag="VFU90_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481109.1"
                     /translation="MTAKRTASQPIISLRRSSRSASDPPISENNEKGATSQMNETPVE
                     SAEWVMWYASSGTASVRSPSPSRLSDCPTKYCRNGRDAKMLGAFATSTIDRVG"
     gene            complement(571..852)
                     /locus_tag="VFU90_14800"
     CDS             complement(571..852)
                     /locus_tag="VFU90_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481110.1"
                     /translation="MPASFQRYRNPSTTSSTRCRAVGTPCTSSGPSDGIRSSDSCDAR
                     YVSALIVNAGSGPKKANNAPPATGVTRYERFSEAISTPIASVDRSRPSK"
     gene            1401..>2286
                     /locus_tag="VFU90_14805"
     CDS             1401..>2286
                     /locus_tag="VFU90_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481111.1"
                     /translation="MPRPRAVAILALLAGLLPSVANAAAITRLPLPGGATIDPFGTRR
                     LMAVSAAGSIAATVTVGGFRTEIVLWNRSGVRLPLASSGGTVAGFDRDGALLVDAGRP
                     HRIAGLRSAPVDTSYCEEFPHSSVGPQLAGVLGNGAAIATMQSPALVNLDDTSGQEAP
                     VVLYLRSRRCLNMGNGIALATAGLYTAGYAAFIQNVPAPSNVISSTERFTAMRWDDRT
                     PQPLGAGVAIAVNASGAAAGADVPPGRGGAYDAAPHARYWSAAGSAVDIAEGAPLSVA
                     YAIDGRDRVTGMLEDAAGR"
BASE COUNT          305 a          770 c          775 g          436 t
ORIGIN      
        1 aggactggct tttgctaaaa agtcggaggc ttcgctctcg gcatccccgc catcgcgcgt
       61 tcgcgaagcg cgggcacgac gcgagtgtcg gtccgagcgg ccgcaccccc accgtaccat
      121 ttcgggcaca ttcttgacga gcgcaagcgg gctgcgctat cctacccggt ctatggtcga
      181 ggtagcgaag gcgccgagca tctttgcgtc gcgcccgttt cggcagtact tcgtcggaca
      241 gtcgctcagc ctgctgggcg acggactccg cacgctcgcg gtgccgctgc tcgcgtacca
      301 catcacccat tcggcgctct cgaccggcgt ctcgttcatc tgcgaggtcg cacccttttc
      361 gttgttctcg ctgatcggcg gatcgctcgc cgaccggctc gaccggcgca agctgatgat
      421 tggctgcgac gccgtgcgct ttgcggtcat ggcgttcttc gcggtggctt actggcagaa
      481 cgttcttacg ttaccgatga tctacggcgg gctggtgatc atctcgatct gcgccgcggc
      541 gttcctgggc gggcagtcgt cgagcattcc ctacttgctc gggcgcgagc ggtcgaccga
      601 agcgatgggc gtgctgatcg cttcggaaaa cctctcgtac ctcgtgacgc cggtcgccgg
      661 cggcgcgtta ttcgccttct tcgggccgct gccggcattg acgatcaacg cgctgacgta
      721 cctcgcgtcg cagctgtcgc tgctgcggat tccgtcgctc gggcccgacg acgtgcaggg
      781 cgttccaacg gcgcggcacc tcgtcgacga cgtcgtgctc gggttccggt acctctggaa
      841 cgacgccggc atgcgtgccc aagcgctggc cggtttcgcg ttcaacgtct tcgggttcgg
      901 ctgttacgcg atcctgatcc cgtttctgaa aaaaggcttc ggcgcgagcg atcaaatcgt
      961 cgggatattt ttcgggatct cggccgcggg ggccgtcgtg ggcgcgtggg tggcctcgcg
     1021 ctgggcaaat cgctggccgt tcggtcgcgc gctgacgacc gcctacgtgc tcgacgggct
     1081 ggcgtttctg ccggtcgtgc tcgtccacaa cgtgtggctg gtcgcgatct tctgggcggc
     1141 atccaacacg ctcgcgaact tcgaaatcgc tcagatcatc ggatttcgca tgcgggtgac
     1201 gcccgagcac atgatcggtc gcgtgatggg cgcggtacgg ctgttcgtgc tctcgggaat
     1261 ggcgccgggc gtgctcgtct tcggttgggt tgccgatcat cgggggccgc tgccggcgat
     1321 gtggatcgcg tgcatcggct acttggtgat cgcgctggtg gcgctcatga cgccggcgat
     1381 ccgcaacgag acgcgcttac gtgccaagac cgcgcgcggt cgcgattctc gcgctgctcg
     1441 ccggcctgtt gccgagcgtg gcgaacgcgg ccgccatcac ccggctgccg ctgcccggcg
     1501 gcgcgaccat cgatccgttc ggtacgcgcc ggctgatggc ggtttcggcc gccgggtcga
     1561 tcgccgcgac ggtaacggtg ggcgggtttc gcaccgaaat cgtgctgtgg aaccgaagcg
     1621 gcgtccgcct tccgcttgcg agttccggag gaaccgtcgc cggcttcgac cgcgacggcg
     1681 cacttttggt cgatgccgga aggccgcacc gcatcgccgg gctgcgcagc gcgccggtcg
     1741 acacgtcgta ttgcgaggaa tttccccatt cgtcggtcgg cccgcagctt gcgggcgtgc
     1801 tcggcaacgg tgcggccatc gcgacgatgc aatcgcccgc cctcgtgaat ctcgacgaca
     1861 ccagcggcca ggaggctccg gtagtcctct acttgcgcag ccggcggtgt ctgaacatgg
     1921 gcaacgggat cgcgctcgcc accgcgggac tctataccgc cggctacgct gcgttcatcc
     1981 agaacgtccc cgctccgtcc aacgtgatct cgagcacgga acgcttcacc gcgatgcgct
     2041 gggacgaccg cactccgcaa ccgctgggtg cgggcgtcgc gatcgcggtc aatgcgtccg
     2101 gagccgccgc gggcgccgac gtgccgccgg gacgcggcgg tgcgtacgac gccgctccgc
     2161 acgcgcgcta ctggtccgcc gccggctccg ccgtcgacat cgcggaaggc gcaccgctga
     2221 gcgtcgccta cgcaatcgac gggcgcgatc gcgtaaccgg gatgctggaa gacgccgccg
     2281 gacggc
//
LOCUS       DASUCJ010001264         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1450026, whole genome shotgun sequence.
ACCESSION   DASUCJ010001264 DASUCJ010000000
VERSION     DASUCJ010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450026"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>1325
                     /locus_tag="VFU90_14810"
     CDS             26..>1325
                     /locus_tag="VFU90_14810"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5481112.1"
                     /translation="MTAAFTNTAPVDAYRGAGRPEATYVVERLVREAAVEMGIAQDEI
                     RRRNFIRKFPYQTPVALMYDTGNYDQTLDGANELADVKGFAARKAEAAKRGRLRGIGY
                     SSYIEACGLAPSNIAGALGARAGLFEAGQIRVNPTGSVTVFTGSHSHGQGHETTFAQV
                     VAGRLGIPVENVDVVHGDTGRVPFGMGTYGSRSLAVGGTAIVKALDKIVGKGKKIAAH
                     LLEAAESDIEFKDGKFTVAGTDRSKTFGEIALTAYVPHNYPLDKLEPGLDETAFYDPT
                     NFTFPAGTHICEVEIDPDTGVVQVVRFSACDDFGNIVNPMIVEGQVHGGLTQGIGQAM
                     LEGCVYDPESGQLLTGSYMDYAMPRADDVPAYHVSTKVTPCTVNPLGTKGCGEAGAIG
                     SPPALINAVLDALASVGVTHLDMPATPHRVWQAIQSAKAAA"
BASE COUNT          233 a          445 c          454 g          193 t
ORIGIN      
        1 cacgacgccg gcaatccact gcgaagtgac ggccgcgttc accaataccg cgccggtcga
       61 tgcctaccgc ggcgcgggtc gccccgaggc gacctacgtc gtcgagcggc tcgtccgcga
      121 ggcggcggtc gagatgggca tagcgcagga cgagatccgc cggcgcaatt tcattcgcaa
      181 gtttccgtat cagaccccgg tcgcgctcat gtacgacacc ggcaactacg atcagacgct
      241 cgacggtgcg aacgagctcg ccgacgtcaa gggcttcgct gcgcgcaagg ccgaggcggc
      301 gaagcgcggc aggctgcgcg ggatcgggta ctcgtcctac atcgaagcct gcgggctcgc
      361 gccgtcgaat atcgcgggtg cgctcggcgc gcgcgcaggg ttgttcgagg cgggccagat
      421 ccgcgtcaac ccgaccggca gcgtgacggt gttcaccggc tcgcacagtc acggccaggg
      481 ccacgagacg acgttcgctc aggtcgtcgc ggggcggctg ggcatcccgg tggagaacgt
      541 cgacgtggtg cacggcgaca ccggccgcgt gccgttcggc atgggcacct acggctcgcg
      601 ctcgctcgcc gtcggcggga cggcgatcgt caaggcgctc gacaagatcg tcggcaaggg
      661 caagaagatc gcggcgcatc tgctcgaagc ggccgagtcc gacatcgagt tcaaggacgg
      721 caagttcacc gtggccggca ccgaccgcag caagaccttc ggcgagatcg cgctcaccgc
      781 ctacgttccg cacaactatc cgctcgacaa gctcgagccg gggctcgacg agacggcgtt
      841 ctacgatccg accaacttca cgtttccggc gggcacgcac atctgcgaag tcgagatcga
      901 tccggacacc ggcgtcgtgc aggtcgtgcg cttcagcgcc tgcgacgatt tcggcaacat
      961 cgtcaatccg atgatcgtcg agggccaggt gcacgggggc ctgacgcagg gcatcggcca
     1021 ggcgatgctc gaaggctgcg tctacgatcc ggagagcgga caactcctga ccggaagcta
     1081 catggactat gcgatgccgc gtgccgacga cgttccggcc taccacgtgt cgacgaaggt
     1141 gacgccgtgc acggtgaacc cgctcggcac caagggctgc ggcgaggcgg gcgcgatcgg
     1201 atcgccaccg gcgctcatca acgcggtgct cgatgcactg gcatccgtcg gcgtcacgca
     1261 cctcgacatg ccggccacgc cgcatcgcgt gtggcaggcg atccagtcgg cgaaggctgc
     1321 ggcct
//
LOCUS       DASUCJ010001265         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_242351, whole genome shotgun sequence.
ACCESSION   DASUCJ010001265 DASUCJ010000000
VERSION     DASUCJ010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242351"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFU90_14815"
     CDS             complement(<1..447)
                     /locus_tag="VFU90_14815"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481113.1"
                     /translation="MLRGAAAAVACALLLCGCGTPGAQSGTLPPLPSAKKGKYFTHIV
                     ILVQENRTFDNLFATFPGADGTTVGKTHDGTMRLRKANLESPLSPNNGYLFWLQDYNN
                     GEMNGFDLVPVGHTPGSYVYQYVDPAQIAPYWTLAKQYVLADHVFQT"
     gene            complement(466..1152)
                     /locus_tag="VFU90_14820"
     CDS             complement(466..1152)
                     /locus_tag="VFU90_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481114.1"
                     /translation="MENVPAERSAGWSVLCYVAALLAASLLTNPLPPPTLTPSDLAIL
                     VDAHRLVILWGAWLTFPASAFFLWFLVGLRSYLRRGTGRQEGLATLAMIAGVVMVAES
                     LFAASLETAVGYTSPDVFQTNGLAAIYSAFIFIQGGLGYAPVSVFLFAAAHSMRRHAT
                     APQWLAVLGYVAAIGTGIATLSIFFDSSFMSPTGTGPGVFGALTSAVWLIATGIVLLR
                     TKEPAAETAA"
     gene            complement(1191..>1598)
                     /locus_tag="VFU90_14825"
     CDS             complement(1191..>1598)
                     /locus_tag="VFU90_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481115.1"
                     /translation="IYADQLGWNAMTQTVAAAYRALPVRQRAQTAIFADRYAYAGAID
                     FYGPRYGLPPVISPNNSYYLWGTRGYSGASVLAVGATDYQLLLRSFGSVRQVAVYRNA
                     YRWVLEGPLPIYLCTQPRAPLAAMWPAFKYYGL"
BASE COUNT          324 a          520 c          494 g          260 t
ORIGIN      
        1 ggtttgaaac acgtggtcgg caagcacgta ttgcttggcc agcgtccagt acggcgcgat
       61 ctgcgccgga tcgacgtatt gatacacgta cgatcccggc gtgtgcccga ccggaacgag
      121 atcgaacccg ttcatctccc cgttgttgta gtcttgcagc cagaataggt agccgttgtt
      181 cggtgagagc gggctttcga gattggcctt ccgcaggcgc atcgttccgt cgtgggtctt
      241 tccaacggtc gttccgtcgg cgccgggaaa ggtggcgaag aggttatcga aggtccggtt
      301 ttcttgaacc aggatcacga tatgggtgaa atatttcccc ttcttggccg acggcagcgg
      361 cggcaaggta ccgctctgcg cgcccggcgt accgcagccg cacaagagca gcgcgcacgc
      421 cacggcggcc gcggcccctc gcagcaccga tcgtccgcgc atctctcacg cagccgtttc
      481 ggcggcgggc tccttcgtcc ggagcagaac gatgccggtc gcgatcagcc acacggccga
      541 cgtgagcgcg ccgaaaactc ccggtccggt gccggtcggc gacatgaacg acgaatcgaa
      601 gaaaatcgaa agcgtcgcga tccccgtgcc gattgcggcc acgtatccga gcaccgcgag
      661 ccactgcggc gcggtcgcat gccgccgcat gctgtgggcc gcggcgaaca ggaacaccga
      721 caccggagcg tagccgagcc cgccctgaat gaagatgaac gcactgtaaa tcgccgccaa
      781 accgttggtt tgaaatacgt cgggcgacgt gtagcccacc gccgtttcga gcgatgcggc
      841 aaacagcgat tccgcgacca taacgacgcc ggcgatcatc gcaagcgtcg ccaaaccctc
      901 ttgccgtccg gtgcctcttc tgagatagct gcgcaacccg accaagaacc aaaggaagaa
      961 cgccgaggcc gggaacgtca gccacgctcc ccataaaata acgagccggt gcgcgtcgac
     1021 gagaatcgcc aaatcggacg gcgtcagcgt cggcggcggc agcggattgg tcagcagcga
     1081 cgcagcgaga agcgccgcta cgtaacagag caccgaccaa ccggcggacc gttcggcggg
     1141 cacgttttcc atacggcacc cttaaccggc gaagaaggct ctcctccccg ttacaatccg
     1201 taatacttaa acgccggcca catcgccgca agcggcgcgc gcggctgcgt acacaggtag
     1261 atcggcagcg gcccttccaa cacccagcgg taggcgttgc gatagacggc gacttgccga
     1321 acgctgccga acgatcgcag cagtaactgg tagtcggtgg ctccgaccgc cagcaccgag
     1381 gcgccgctat agccgcgggt gccccacaga tagtaggagt tattgggact tattaccggc
     1441 ggcaaaccgt aacgcggccc gtagaagtcg atcgctccgg cgtatgcgta cctatccgcg
     1501 aaaatcgccg tttgcgcgcg ctgtcgcacc ggaagtgccc ggtacgcggc cgccaccgtc
     1561 tgcgtcatcg cgttccagcc gagctgatcg gcgtagat
//
LOCUS       DASUCJ010001266         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_333963, whole genome shotgun sequence.
ACCESSION   DASUCJ010001266 DASUCJ010000000
VERSION     DASUCJ010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333963"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFU90_14830"
     CDS             <1..948
                     /locus_tag="VFU90_14830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU5481116.1"
                     /translation="NRLCGSGLQAVASAAQAIRAGEGDVFVAGGVESMSRSPWVMLKP
                     EEGFPRSAPVAADTTVGWRFTNPKMPGEWTISLGETAEEVATRCGVSREEQDAFALES
                     QRRAACAMADGRFDEEIVPVTVPGRRGDATVVSRDEHPRPDVTLQSLAAMKPAFVKQN
                     GTVTAGSSSGINDGASALLVMSEPAAQRAGLQPMARIVASAVAGVSPEVMGLGPVPAT
                     RKVLARAGLRMSDIDLVELNEAFASQSVACMRELGCDPARTNVNGGAIALGHPLGASG
                     ARVLTTLVHEMRRREAARGLATMCIGVGQGIAMIVERVR"
     gene            945..1745
                     /locus_tag="VFU90_14835"
     CDS             945..1745
                     /locus_tag="VFU90_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5481117.1"
                     /translation="MSYEHIRVEREDGIGTLILNRPDKLNAFAGTMRREVADALDELE
                     HDDAVRVIVLTGAGRAFCAGADIGYMSELIATRDEAAMAALVEAGRRVVLTMRASSKP
                     VIGSINGAAAGGGANLALACDLRIASDRASLGQTFNRIGLHPDWGGTYFLPRLVGPAK
                     TLELFWLAEMVDAAEGLRLGLFNRVVPHDALGTETRALAAALAAKPPLAIAMAKRAVY
                     ASLDRSLPEMLDYELDAQLRCFRSGDAAEGITAFLEKRRPAFGQPAAR"
     gene            1803..2918
                     /locus_tag="VFU90_14840"
     CDS             1803..2918
                     /locus_tag="VFU90_14840"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU5481118.1"
                     /translation="MALTKITNFDDWVDLFKVWQDDIGLEARELKDYRFDVKLADLDV
                     PEIEFGHYRGNHKWPTVMHIPDQRIRDALQTLIIFQGDTEFASVEQQRALLEHAPSDY
                     DLLAIYRVMAEEMRHGWQMSYLLVSHFGDEGKREAEKLLQRRADEHERLLGSFNESVE
                     NWLDFFAYTEFIDRDGKYQLQMMSTSAFAPLSRSMKPMLREESFHLGTGNNGLLRIIK
                     AGRIPTDVMQRYFNKWVSTAFDLFGTDGSSSAQWAYTWGLKGRFDERTNPQSPDPARL
                     NEYARELYRQEVQGLIDRLNLHVPEHQPKLKVPSIKFNRRIGQYKGQTFNLDGEPIPR
                     EGYKAYVESVMPTAADRELLRSIFKENDWIQPKKSDD"
     gene            2908..3672
                     /locus_tag="VFU90_14845"
     CDS             2908..3672
                     /locus_tag="VFU90_14845"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5481119.1"
                     /translation="MTTNASGPESGAGPARRAGPLLIGHRGAPRERPENTLPSFMRAL
                     ELGADGIELDVHATKDGVVVVHHDEVPRAAAPIGRLTGKRIDALTFDELQGFSVRGMA
                     LIPTLAEVLAAVRGRASVFIELKGAGVEAAAIDVIRKSPSPDRCAVHSFDHDAVRRAR
                     AIAPEIRGGILFDRALGDVPAAMRAADALDAWPRWDLIDAGLVAAVHGAGGRVIAWTV
                     NRTERATALAALGVDGLCTDHLPLLQQILRPAALSS"
BASE COUNT          604 a         1179 c         1419 g          591 t
ORIGIN      
        1 aaccgcttgt gcggttccgg tctgcaggcc gtggcgagcg cggcgcaggc gattcgcgcc
       61 ggtgaaggcg atgtgttcgt ggcgggcggc gtcgagagca tgtcgcgctc gccgtgggtc
      121 atgctcaagc cggaggaggg attcccccgc tccgctccgg tcgccgcgga caccaccgtc
      181 gggtggcggt tcacgaatcc gaagatgccg ggcgagtgga cgatttcgtt aggcgagacg
      241 gcggaggagg tggcgacgcg ctgcggcgtg tcgcgggagg agcaggatgc gttcgcgctc
      301 gagagccagc gacgagcggc gtgcgcgatg gccgatgggc gcttcgatga ggagatcgtg
      361 ccggtgacgg tgccgggacg ccgcggtgac gcgacggtcg tgtcccgcga tgaacacccg
      421 cgtcccgatg tgacgctgca gagtctcgcg gccatgaagc cggcgttcgt gaaacagaac
      481 ggcaccgtca ccgcggggtc gtcgagcggg atcaacgatg gcgcgagcgc tttgctcgtc
      541 atgtcggagc cggcggcgca gcgagccggg cttcagccga tggcacggat cgtcgcgtcg
      601 gcggtggccg gggtgtcgcc cgaggtgatg gggttgggtc cggtgccggc gacgcgcaag
      661 gtgctggcgc gagccggcct tcggatgtcg gacatcgatc tcgtcgagct caacgaagcg
      721 ttcgcgtcgc agtcggtggc gtgcatgcgc gagctcggat gcgaccccgc gcgcacgaac
      781 gtgaacggcg gcgcgattgc gttgggccat ccgttaggcg cgtcgggcgc ccgggtcctg
      841 acgacgctgg tgcacgagat gcggcggcgg gaggcggcgc gcggcctggc gacgatgtgc
      901 atcggcgtcg gccaggggat cgcgatgatc gtggagcgcg tgcgatgagc tacgagcaca
      961 ttcgcgtcga gcgcgaggat ggcatcggga cgttgatcct caaccgcccc gacaaactga
     1021 atgcgttcgc cgggacgatg cgccgcgaag tggccgatgc gttggacgag ctcgagcacg
     1081 acgacgccgt gcgcgtgatc gtgctcaccg gcgcggggcg cgcgttctgc gccggcgccg
     1141 acatcggcta tatgtccgag ctcatcgcca cgcgcgacga agcggcgatg gcggcgttgg
     1201 tcgaagccgg ccggcgcgtg gtcctgacga tgcgggcgtc ctccaaaccg gtgatcggaa
     1261 gcatcaacgg tgcggcggcg ggcggcggcg ccaacctcgc gctggcctgc gacctccgca
     1321 tcgccagcga ccgggcatcg ttaggccaaa ccttcaaccg catcggcctg catccggatt
     1381 ggggcggcac gtacttcctg ccgcggctgg tcggtcccgc gaaaacgctc gagctgttct
     1441 ggctggcgga gatggtcgac gccgccgaag gtctgcggct cggattgttc aaccgcgtgg
     1501 tgccgcacga tgcgttaggc acggagacgc gcgcgctggc ggcggcgctc gccgcgaagc
     1561 cgcccctcgc catcgcgatg gccaagcgcg ccgtgtacgc gtcgctcgac cggtcgctgc
     1621 ccgagatgct cgactacgag ctggacgcgc agctccggtg cttccggagc ggcgacgcgg
     1681 cggaaggcat cacggcgttt ctggagaagc ggcggccggc gttcggtcag ccggcggccc
     1741 gttgacacac cggccccgtt aggcgtgggg ccggaggcag ttcaccagag gagcaccaca
     1801 tcatggcgtt gacgaaaatc acgaacttcg acgactgggt ggacctgttc aaggtctggc
     1861 aggacgacat cggtctcgag gccagggagc tcaaggacta caggttcgac gtgaagctcg
     1921 ccgatctgga cgtgcccgag atcgagttcg gccactaccg gggcaatcac aagtggccca
     1981 ccgtgatgca catcccggat cagcgcatcc gcgacgcgtt gcaaacgctc atcatctttc
     2041 agggcgacac cgagttcgcg tcggtggagc agcagcgcgc gctgctcgag cacgcgccga
     2101 gcgactacga tttgctcgcg atctatcgag tgatggccga ggagatgcgg cacggatggc
     2161 agatgtcgta tctgctcgtg tcgcacttcg gcgatgaggg caagcgcgag gccgaaaagc
     2221 tgctccagcg ccgcgccgac gagcacgagc ggctgttggg ctcgttcaac gagagcgtcg
     2281 agaactggct cgacttcttc gcctacaccg agttcatcga ccgcgacggc aagtaccagc
     2341 tgcagatgat gtcgacgtcg gcgttcgcgc cgctgtcgcg atcgatgaag ccgatgttgc
     2401 gcgaagagtc gttccattta ggcacgggca acaacgggct cctgcgcatc atcaaggcgg
     2461 ggcgcattcc gaccgacgtc atgcagcgct acttcaacaa atgggtgtcg acggcgttcg
     2521 atctgttcgg caccgatgga tcgtcgtcgg cgcagtgggc gtacacgtgg gggctcaagg
     2581 gacggttcga cgagcggacg aacccgcagt cgcccgatcc ggcgcgtctc aacgagtacg
     2641 cgcgcgagtt gtaccggcag gaggtgcagg ggttgatcga ccggttgaac ctgcacgtgc
     2701 cggagcatca gccgaagctc aaggtgccga gcatcaagtt caatcggcgc atcggccagt
     2761 acaaagggca gacgttcaac ctggacgggg agccgattcc gcgcgagggc tacaaggcgt
     2821 atgtggaatc ggtgatgccg acggcggcgg accgggagct gttgcgctcg atcttcaagg
     2881 agaacgactg gatccagccc aagaagagtg acgactaacg caagcggacc ggagtcgggc
     2941 gcggggccgg cgcgccgggc gggtcccctg ctcatcgggc accgcggcgc gccgcgggag
     3001 cggccggaga acacgctgcc gtcgttcatg cgcgcgctcg agttgggcgc cgacggcatc
     3061 gagctcgatg tgcacgccac gaaagacggc gtggtggtgg tgcaccacga cgaggtcccc
     3121 cgcgcggcgg cgccgatcgg ccgcctaacg ggaaagcgca tcgatgcctt gacgttcgat
     3181 gagctgcagg gcttttcggt gcgcggcatg gcgctcatcc cgacgcttgc cgaggtgttg
     3241 gcggcggtgc gcgggcgcgc cagcgtgttc atcgagctca agggcgcggg cgtggaagcg
     3301 gcggcgatcg acgtgatacg gaaatcgcct tccccggacc gatgcgcggt gcacagcttc
     3361 gaccacgacg ccgtgcggcg ggcgcgtgcg atcgcgccgg agattcgcgg ggggatcctc
     3421 ttcgaccgtg cgctgggcga cgtgccggcg gccatgcgtg ccgccgacgc gctggacgcg
     3481 tggccgcggt gggatctcat cgatgccggg ctcgtggcgg cggtgcacgg cgccgggggg
     3541 cgcgtgatcg cctggacggt gaaccggacc gagcgggcga ccgcgctggc tgcgttaggc
     3601 gtggacggcc tgtgtacgga tcatcttccc ctcctgcagc agatcctgcg gccggcggcg
     3661 ctgtcatcgt gacgcgcacc atgccggtcg ttgccgtcat cggcgcgggc acgatgggcc
     3721 acggcatcgc ctacgtcgcc gccctcgccg gctcccgcgt attcctaacg gacgtcagcg
     3781 ccgagcagct ggc
//
LOCUS       DASUCJ010001267         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_288362, whole genome shotgun sequence.
ACCESSION   DASUCJ010001267 DASUCJ010000000
VERSION     DASUCJ010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288362"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFU90_14850"
     CDS             <1..1017
                     /locus_tag="VFU90_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481120.1"
                     /translation="VHNPLPYYDFHLGPITLKAQLHHHAEALSQYFFDREKWRGLFLP
                     GLHFGFGWAYPVVLGLALAGLILGLWRAPTTLHRLGALAYLVGLIAFVFTPYTADGGG
                     LSFKFNLRYMIPSLLGAVVYYGFAFRAHRVVWRWVVMGLMLGVIVIAMFARNYERVPA
                     WPRHKYIGVPGAVFGVLALIAIWYFVIRPKRWKLLGLIAMVVVVGAFAIGWPLQRHYF
                     DNRYVHARLPLDEIYAPFRDIRHASVAYYGTLETYPFYGPDLSNHVARPPAPEVFDRI
                     GRCRAWLDELSKYQYIAVTHQPFAYDAPPEFAIAHDRSVSTVAGSGLSVLYRVNRPLE
                     ANAC"
BASE COUNT          139 a          345 c          321 g          217 t
ORIGIN      
        1 gtgcacaacc cgcttccgta ctacgacttc catctcgggc cgatcacgct caaagcgcag
       61 ctgcaccatc acgccgaggc gttgtcgcag tacttcttcg accgggagaa gtggcggggc
      121 ctcttcctcc ccggtctgca cttcggtttc ggttgggcgt atccggtcgt gctcggtctc
      181 gcgctcgcgg gcttgattct cggattgtgg cgcgcaccga ccacgctgca ccggctgggc
      241 gcgctcgcgt atctcgtcgg gctgatcgcg ttcgtgttca cgccgtacac ggctgacggc
      301 ggcggcctgt cgttcaagtt caatctgcgc tacatgatcc cgtcgctgct cggcgccgtc
      361 gtgtattacg gcttcgcctt ccgggcgcac cgtgtcgtgt ggcgatgggt cgtgatgggc
      421 ctgatgctcg gcgtgatcgt gatcgccatg ttcgcgcgca actacgagcg tgttcccgcg
      481 tggccgcgcc acaagtacat cggcgtcccc ggagcggtgt tcggcgttct cgcactgatc
      541 gcgatctggt acttcgtcat ccggcccaag cggtggaagc tgctgggcct gattgcgatg
      601 gtggtggtcg tcggcgcgtt cgcgatcggc tggccgctcc agcgtcacta cttcgacaac
      661 cgctacgtgc acgcacgcct gccgctcgac gagatctatg cgccgttccg cgacatccgg
      721 cacgcgagtg tggcttatta cggcacgttg gagacgtatc cgttctacgg tcccgacctc
      781 tcgaaccacg ttgcccgtcc acccgcgccc gaggtgttcg accggatcgg ccgctgccgc
      841 gcctggctcg acgagttgag caagtatcag tacatcgcgg tcacgcatca gcccttcgca
      901 tacgacgcgc cacccgagtt cgcgattgcg cacgaccgct ccgtgtcgac ggtcgccggg
      961 agcggactgt cggtcctcta ccgcgtgaac cggccgctcg aagcgaatgc ttgctgaacg
     1021 cg
//
LOCUS       DASUCJ010001268         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_609226, whole genome shotgun sequence.
ACCESSION   DASUCJ010001268 DASUCJ010000000
VERSION     DASUCJ010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609226"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VFU90_14855"
     CDS             <1..1489
                     /locus_tag="VFU90_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481121.1"
                     /translation="TMPPIHAGIGRPKPRLAAYALAEAASKTIGGSTSRVGADLSIPI
                     TPTASFVATFHPDYSNVELDQQSISPTVYARYYAEVRPFFTQGANFYGQFDCDACPNI
                     QELYTPAIPTPREGYAVEGQQGPLSFATFDALGDAGRSDIASALDYRSTDNRWRAVVQ
                     RVQVNTDTLVDDVTTTGIAYYDLKHISAYFNYGSDSGTNVLTPDQAQRYDFGGGWANQ
                     SFGFFGSVRKLGEYYNPVDGFVSHPGIAGYALYSAKIWTFAPSSKLAAIGVAGFMDRY
                     QGPTQGQAQSDNSLLLDVLTKSAWDLQLFSGSNYWRFNDPLTGLGTLTPVSQNAGFQL
                     TYHSGLQSNNPGQFPYHGSSSDPTQLAYNTGRYGTGTLDTWYRSTTLRVGSRGALTLV
                     LDSTQQWFPNATPNVQWFESASYAFQITHNSSFGIGVRRVVGTPPNPNGGGDCVGTCS
                     NISVAYHLLTRHAEFYLAYGNPNALITVPQAIFKVIFYAGAEKGT"
     gene            1626..2276
                     /locus_tag="VFU90_14860"
     CDS             1626..2276
                     /locus_tag="VFU90_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481122.1"
                     /translation="MKRIIMALAGALLAASALPAQAASSLDVPTASAAPSVDPRADDT
                     SFDPAAVAQLGWNSTKSQPAAEDTTARIATDGKYLYVRFDAAQTERIVSAQPADGKST
                     GDLVWVDLQPGSSGASYRFASSPDGSSNATASTGTAPAFAAAGSTYDGGYTVTMKIPI
                     ADLHGAGGTWNVQFGRTIAGSGQQMVWSHDGNVAQAGTMTMPASVGAAAPAGATRE"
     gene            2589..3467
                     /locus_tag="VFU90_14865"
     CDS             2589..3467
                     /locus_tag="VFU90_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481123.1"
                     /translation="MLILALAIAVPVAPTVAAGTGATPAPTRTPPPQIYRIITRPLCS
                     ELHKHIAPAIALMLENDNNIKKGPELFSRYNRDALTGPDNSASNGAGPGGPVMSGESG
                     GSNMTAAQNMALLGMENLVSPIANNIIATQKLLDSPALTQGTGNPEDDKQLQEMRDKL
                     LKALATQNAALDIISGFVATQQMGDLQHAGQEYISAINQGDVTGRGSGATPTPNPLLT
                     NPDQAGLPANPYDLNLANVPGLTLGYNPVTRLLDALHWTIQETQTRENEAAKSVMSGA
                     QACAASTPPPTTPKPH"
BASE COUNT          681 a         1236 c         1068 g          526 t
ORIGIN      
        1 gacgatgccg ccgattcacg ccgggatcgg gcggccgaaa ccgcggctgg cggcgtacgc
       61 gctggccgaa gcggcgtcga agacgatcgg cggctcgacc agccgggtcg gcgcggattt
      121 gtcgatcccg atcacgccga cggcgtcgtt cgtcgccacg ttccaccccg actattccaa
      181 cgtcgagctg gatcagcagt cgatttcgcc gaccgtgtac gcgcgctact acgccgaggt
      241 gcggccgttc tttacccaag gcgcgaactt ctacgggcag ttcgactgcg acgcatgccc
      301 gaacatccaa gagctctaca cgccggcgat tccgacgccg cgcgaggggt acgcggtgga
      361 agggcagcag gggccgcttt cgttcgccac cttcgacgcg ctcggcgacg ccggccgcag
      421 cgacatcgca agcgcgctcg actaccgctc gaccgacaac cgctggcgcg cggtcgtgca
      481 gcgcgttcaa gtaaataccg acacgctggt cgacgacgtc accaccaccg ggatcgcgta
      541 ctacgatctc aagcacatct cggcgtactt caactacggc agcgactcgg gcacgaacgt
      601 gctcacgccg gatcaagcgc agcgttacga cttcggcggc ggctgggcga accagagctt
      661 cggattcttc ggttcggtcc gcaagctcgg cgagtattac aatccggtcg acggcttcgt
      721 atcgcatccg gggatcgccg gctacgcgct ctacagcgcc aagatttgga cgtttgcgcc
      781 gtcgagcaag ctcgccgcga tcggggtcgc cggcttcatg gaccgctacc aagggccgac
      841 gcaggggcag gcgcagagcg acaactcgct gctgctcgac gtcctcacca agagcgcgtg
      901 ggacctgcag ctgttctcgg gatcgaacta ctggcgcttc aacgatccgc tcaccggact
      961 ggggacgttg acgccggttt cacagaacgc cggcttccag ctgacctacc acagcggact
     1021 gcagagcaac aatccggggc agttccccta ccacggttcg tcgtcggatc cgacgcagct
     1081 agcatacaac accggccgct acggcaccgg cacgctcgac acgtggtatc gctcgacgac
     1141 gctgcgcgtc ggctcgcgcg gtgcgctcac cttggtgctc gacagcacgc agcaatggtt
     1201 tccgaatgcc acccccaacg tgcaatggtt cgaaagcgcg agctacgcgt ttcaaatcac
     1261 gcacaactcg tcgttcggaa tcggcgtgcg gcgggtcgtc ggcacgccgc ccaaccccaa
     1321 cggcggcggc gactgcgtcg gcacctgttc caacatttcc gtcgcctatc acttgctcac
     1381 gcggcacgcc gagttctact tggcgtacgg aaatccgaac gccctgatca ccgtgccgca
     1441 agcaatcttc aaagtcatct tctacgcagg cgcggagaaa ggaacgtaac gtgtcaacgc
     1501 aatggcacac gaaacttcat tcgtgggttc gcgtacatgt gacaagtgcg tgctcgacgc
     1561 gtgcgtgttg tgccaacaca tcgtcaccga ttttgccact cagcatccat aggagaaacg
     1621 ctaccgtgaa acgcatcatc atggcgctcg ccggagcgct gctggcggcg agcgccctcc
     1681 ccgcgcaagc cgcctcgtca ctcgacgtcc ccaccgcatc cgccgcacct tcagtcgacc
     1741 cacgggccga cgatacgtcg ttcgaccccg cggcggtcgc gcaactcggc tggaacagca
     1801 ccaaatcgca acccgccgcc gaagacacca ccgcacgcat cgcgaccgac ggcaagtacc
     1861 tctacgttcg cttcgacgcg gcgcaaacgg aacgcatcgt gtcggcgcag cctgccgacg
     1921 gcaagagcac cggcgacttg gtctgggtcg atctgcagcc cggatccagc ggcgccagct
     1981 atcgcttcgc ctcgagtccc gacggctcga gcaacgccac cgcgtcgacc ggcaccgcgc
     2041 ccgcattcgc agcggccgga agcacgtacg acggcggcta caccgtcacg atgaagatcc
     2101 cgattgccga cctgcacggt gccggcggca cgtggaacgt gcagttcgga cgcacgatcg
     2161 ccggatcggg ccagcagatg gtctggtcgc acgacggcaa cgtcgcgcaa gccggcacga
     2221 tgacgatgcc cgcgtcggtc ggcgccgcgg cgccggccgg ggcgacgcgc gaataacccg
     2281 tccccacccc acggagcgca cgtcctagcg ccttcaaagt ttcctcacat ttctcgcgca
     2341 agctgcgaga gaggcgactt tggaggcgcg cgctccgtta ctcgcaacgg agggaccact
     2401 cgtgccgctg cagactgttc gcacggccgc gctcgcatag gagaagctgc gcagtcgcga
     2461 cccaggcagc ctgcagcggg cccttgcata gccgcccctt gccgctgggg tgacgatcgg
     2521 cgtaacaagc aggcgcgacg gggcgttctt ggtgtatagt ggctccgatg cataaggctc
     2581 tccctgcggt gctgatcctg gcactggcca tcgccgtgcc cgtcgcaccg accgtcgccg
     2641 ccggcaccgg cgcgacgccc gccccgaccc gcacgccgcc cccgcagatc taccgcatca
     2701 tcacccggcc gctgtgttcg gagctgcaca agcacatcgc cccggcgatc gcgctgatgc
     2761 tcgagaacga caacaacatc aaaaaaggtc ccgagctgtt cagccgctac aatcgcgacg
     2821 cgttgaccgg tcccgacaac agcgcgagca atggcgccgg tccgggcggg ccggtgatgt
     2881 cgggcgagtc cggcggcagc aacatgacgg cggcgcagaa catggcgttg ctggggatgg
     2941 agaacctcgt cagcccgatt gccaacaaca tcatcgcgac gcaaaaactg ctcgactcac
     3001 ccgcgctgac gcaaggcacc gggaaccccg aagacgacaa gcagctgcaa gagatgcgcg
     3061 acaagctgct caaggcgctg gcgacccaga acgccgcgct ggacatcatc agcggattcg
     3121 tcgcaacgca gcagatgggc gacctgcagc atgcggggca agagtacatc agcgcaatca
     3181 atcagggcga cgtcacggga cgaggcagcg gcgccacccc gacgcccaac ccgctgctca
     3241 ccaatccgga tcaagccgga ttgccggcca atccgtacga cctcaacctc gccaacgttc
     3301 cggggctgac gctcggctac aatccggtca cccggctgct cgacgcgctg cattggacga
     3361 ttcaagaaac gcaaacgcgc gagaacgaag ccgcgaaatc ggtgatgtcg ggagcgcagg
     3421 cgtgtgcggc ctcgacgcca ccgccgacga cgccgaaacc gcactagccc ttcgactccg
     3481 gcgctgcgcg cctccgctca ggatgacaac c
//
LOCUS       DASUCJ010001269         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_151298, whole genome shotgun sequence.
ACCESSION   DASUCJ010001269 DASUCJ010000000
VERSION     DASUCJ010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151298"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFU90_14870"
     CDS             <1..641
                     /locus_tag="VFU90_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481124.1"
                     /translation="RVRALVARMFRLDHDLSPFYALIAGDPQLGWAAAGAGRMLASPT
                     VFEDVVKTICTTNCAWSGTIRMTRALAELGGGAFPEPEILAGAPDAWFRDVARMGYRG
                     PYLRTIAREVAAGRLDLERLLPDRGLDDATVEEILLTLPGVGPYAAAHVMQLLGRHRR
                     LVLDSWTRPTYLRLAKKKRAADSSINRAFARYGEYAGLAFWLTLTRGWVTEP"
     gene            742..>1157
                     /locus_tag="VFU90_14875"
     CDS             742..>1157
                     /locus_tag="VFU90_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5481125.1"
                     /translation="MRWLKAMAGACLFVVSLGGNAVAGTTGGIVGRVVDSQTSAPVAG
                     AVVTVTAPSQTATAVTDAAGAYRFLTLVPDTYTVSVAKQGYDPFTQAGLSVFADQVQT
                     FNIRIVATLRTIAHVTASAPANLVKPGTTSDVYSINA"
BASE COUNT          197 a          373 c          384 g          203 t
ORIGIN      
        1 atcgggttcg cgcactggtg gcgcgaatgt ttcggctcga tcacgaccta tcgccgttct
       61 acgcgttgat tgccggcgac ccgcagctgg gttgggcagc cgccggcgcc ggccggatgc
      121 tggcgagtcc aaccgtgttt gaagacgtcg tgaagacgat ctgcaccacg aactgcgcgt
      181 ggtcgggcac gattcggatg acgcgcgcgc ttgcggagct cggcggcggc gcgttccctg
      241 aaccggaaat cctggcaggc gcgccggatg cgtggttccg cgacgtcgcc cggatgggtt
      301 atcgcggacc gtacctccgg acgattgcgc gcgaggttgc agccgggcgt ctagatttgg
      361 aacgcctcct tcccgaccgc ggcctcgacg acgccacggt cgaggagata ctgctgacgt
      421 tgccgggcgt tggcccgtac gcggcagcac acgtcatgca gttgctcgga cggcatcgcc
      481 ggctggtcct cgattcgtgg acgcgcccca cctatttgcg gctggcgaag aagaagcggg
      541 ctgccgattc gagcatcaac cgcgccttcg cacgctacgg cgaatacgcc ggtttggcat
      601 tctggctgac gctcacgcga ggctgggtaa ccgaaccgta gtccgcatgg cccgctcgga
      661 tggccgggca gaggggcgag cggttttctg ccgataaccc tttcgcagac gagagtcgcc
      721 gcgaaaggag cgtacgatcc gatgcgatgg ctgaaagcaa tggcgggcgc gtgcctcttc
      781 gtcgtttcgc tcggcggaaa cgccgtagcc ggaacgaccg gcggcatcgt cggacgagtc
      841 gtagattcgc aaaccagcgc accggttgcg ggcgccgtcg tcaccgtaac ggcaccctcg
      901 caaacggcga cggccgtgac cgacgccgcc ggggcttacc ggtttcttac gctcgttccc
      961 gacacgtata ccgtgtcggt cgccaaacaa ggctacgacc cgtttaccca agcgggacta
     1021 tccgtgttcg ccgaccaggt gcagacgttc aatatccgga tcgttgcgac gcttcgcacg
     1081 atcgcgcacg tgacggcgag cgcaccggcc aatctcgtca agccgggaac gaccagcgac
     1141 gtctactcga tcaacgc
//
LOCUS       DASUCJ010001270         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1129167, whole genome shotgun sequence.
ACCESSION   DASUCJ010001270 DASUCJ010000000
VERSION     DASUCJ010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129167"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFU90_14880"
     CDS             complement(<1..250)
                     /locus_tag="VFU90_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5481126.1"
                     /translation="MADVCVVRGLRKTFEAELAPVRALRGVDLDVAAGEFVAIMGPSG
                     CGKSTLLNLVAGLDIADEGEITIAGDAVTGRNEDELARM"
     gene            complement(250..>1097)
                     /locus_tag="VFU90_14885"
     CDS             complement(250..>1097)
                     /locus_tag="VFU90_14885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5481127.1"
                     /translation="EEQLLQWIAEGRPVKAIAIAWHTTPEAANDAIEELFLKLAKEAS
                     AGREGALRRLRLLQKAIVDREEQGETLRRLLPGGLAEKLRSGGRSIGETETMIVTVLM
                     SDVRGYSGIAEHVDPTALAAMLNTHRAEMNAAILAEGGTVMQYVGDAVMAVFGAPFPQ
                     ADHADRALAAACAMLERQRAVNEKWRADGLPEFGLGIGLSTGEVAAALLGSDERVEYT
                     LVGDTVNLAQRLQDLARPAGTIVLSGATSDALSTRPALDLLGEQQVKGRETPVLAYRL
                     HKENN"
BASE COUNT          162 a          402 c          342 g          191 t
ORIGIN      
        1 gcatgcgcgc gagctcgtct tcgttgcggc ccgtcaccgc gtcaccggca atcgtgatct
       61 cgccctcgtc ggcgatgtcg aggccggcga cgagattgag cagcgtcgac ttcccgcagc
      121 ccgacgggcc catgatcgcg acgaactcgc ccgcggcgac gtcgagatcc acaccccgca
      181 acgcgcgtac gggcgccagc tcggcttcga acgtcttgcg aaggccgcga acgacgcaca
      241 cgtctgccat cagttgttct ccttgtggag ccggtacgcc agcacgggcg tctcgcggcc
      301 cttcacctgt tgttctccga gcagatcgag tgcgggacgc gtcgacaacg cgtccgacgt
      361 cgcgccgctc aacacgatcg ttccggccgg acgcgcgagg tcctgtaacc gttgggcgag
      421 attcaccgtg tccccgacca gcgtgtactc gacgcgttcg tcggaaccga gcagcgcggc
      481 cgcgacctcg ccggtcgaca agccgatgcc gagcccgaac tccggcaagc cgtccgcccg
      541 ccacttctcg ttcacggccc gctggcgttc gagcatcgcg cacgccgccg cgagcgcgcg
      601 gtcggcatgg tctgcttgcg ggaacggtgc gccgaacacc gccatcaccg cgtcgcccac
      661 gtactgcatc acggtgccgc cctcggccag gatcgcggcg ttcatctcgg cgcgatgagt
      721 gttgagcatc gcggcgagcg cggtcggatc gacgtgctcg gcgatgccgg agtacccgcg
      781 gacgtccgac atcagcacgg tcacgatcat cgtctcggtc tcgccgatcg aacggccacc
      841 gctgcgcaac ttctcggcga ggccgcccgg cagcagtcgc cgcaaggtct cgccctgttc
      901 ttcgcgatcc acgatcgcct tctgcaacaa gcgcagtcgc cgcaacgcgc cctcccgacc
      961 ggcgctcgct tctttcgcga gtttcaagaa cagttcttcg atcgcgtcgt ttgccgcttc
     1021 gggtgtggtg tgccacgcga tggcgatcgc cttcaccggg cggccttcgg cgatccactg
     1081 caagagttgt tcttcgc
//
LOCUS       DASUCJ010001271         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_28764, whole genome shotgun sequence.
ACCESSION   DASUCJ010001271 DASUCJ010000000
VERSION     DASUCJ010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28764"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFU90_14890"
     CDS             complement(<1..525)
                     /locus_tag="VFU90_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5481128.1"
                     /translation="MSEERAVLHRRLIDALGSDAVKTAPEDLSVYAFDAYTDGPLPSA
                     VVLPASVREVSAAVKIAREFGEPIVARGAGTGLCGGAVPVAGGIVIALARMNRILELD
                     VPNRRARVESGVINLDLSKDAAPSGLFYAPDPSSQKISTIGGNVATNAGGPRSLRHGP
                     VRSWLSGVELVLPDG"
     gene            complement(522..>1384)
                     /locus_tag="VFU90_14895"
     CDS             complement(522..>1384)
                     /locus_tag="VFU90_14895"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU5481129.1"
                     /translation="ASMLTGIDVLLFDIQDVGSRAYTYVSTMAYAMQSARRYGKEFWV
                     LDRPNPVGGLLVEGPVLEPAYESFIGLYPIAMRHGMTVGELARLFNDRFGIGANLRVI
                     PMNGWRRSMIWPDTGLQWIRTSPNVPQWQTTFVLLCTGLIDNAGINNGVGTNRPFFYA
                     GMIGIDGRRLAVALEARALPGVTFQPAVWSPSAGFWKDRELPGVELTITDFRSFLPVR
                     TAVEILVTIQRLFPHAIDVKAAALDRDWGTASLRQGLLAGSPADAIVAGWRDSLAAFR
                     ALRAKYLLYE"
BASE COUNT          251 a          460 c          454 g          219 t
ORIGIN      
        1 gccgtcgggc agcacgagct cgacgccgct cagccacgag cgcaccgggc cgtggcgcag
       61 cgagcgcggg ccgccggcgt tggtggcgac gtttccaccg atggtcgaaa ttttttgcga
      121 tgaaggatcc ggcgcgtaga agagcccgct cggggcggcg tctttggaca gatcgaggtt
      181 gatgacgccg gactcgacgc gcgcgcggcg attgggcacg tcgagctcaa gaatccggtt
      241 catgcgcgcg agcgcgatta cgatgccgcc ggcgactgga accgcgccgc cgcacagccc
      301 ggtgccggcg ccgcgcgcga cgatcggctc gccgaattcg cgcgcgatct tgaccgccgc
      361 gctcacctcg cgcaccgagg ccggcaagac taccgccgac ggcaacggcc cgtcggtata
      421 cgcatcgaag gcgtagaccg aaagatcttc cggagcggtc ttgacggcgt cgctcccgag
      481 cgcgtcgatc agccggcggt gcagcaccgc acgttcttcg ctcattcgta cagcagatac
      541 ttggcgcgca gcgcgcgaaa tgcggccaag ctatcgcgcc atccggcgac gatcgcgtcg
      601 gccggcgatc cggccaagag gccttgccgc agggaggcgg ttccccagtc acgatcgagg
      661 gcggcggcct tgacgtcgat tgcgtgcgga aacaaccgtt gaatcgtcac cagaatttcg
      721 accgcggtgc gcaccggaag gaagctgcga aagtccgtta tcgtaagctc gacgccggga
      781 agctcgcgat ctttccaaaa gccggccgac ggcgaccaca ccgccggctg aaacgttacg
      841 cccggcaagg cgcgcgcttc gagcgcgacg gccaaccggc gtccgtcgat cccgatcatt
      901 ccggcgtaga aaaacgggcg gttcgttccc acgccgttgt tgatgcccgc gttgtcgatc
      961 agccccgtgc agagcagcac gaaggtcgtc tgccactgcg gcacgttggg ggacgttcga
     1021 atccactgca agccggtgtc gggccaaatc atcgatcgcc gccagccgtt catcggaatg
     1081 acgcgcaggt tcgcgccgat gccgaagcga tcgttgaaca accgcgctaa ctctccgacc
     1141 gtcattccgt gccgcatcgc gatcgggtag aggccgatga acgactcgta ggccggttcc
     1201 agcaccggtc cttcgacaag cagtccgccg accgggttgg gacggtcgag cacccagaac
     1261 tctttgccgt accgtcgggc gctctgcatc gcatacgcca tcgtagagac gtaggtgtac
     1321 gctcgcgagc cgacgtcttg gatgtcgaac agcagcacgt cgattcccgt cagcatcgag
     1381 gcgc
//
LOCUS       DASUCJ010001272         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_59708, whole genome shotgun sequence.
ACCESSION   DASUCJ010001272 DASUCJ010000000
VERSION     DASUCJ010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59708"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFU90_14900"
     CDS             complement(<1..511)
                     /locus_tag="VFU90_14900"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5481130.1"
                     /translation="MKLYISSDMEGVAGVCGWEQVDARTPHPEYAIYRRYYTQEVESA
                     IRGARAAGAGEVLVNDSHGPMRNLLFDELPHDVRVLFGNRKPFSMVQDADASFGGAFF
                     IGYHGGAGEADAVLCHTYTPSVIYEVRVNGIRCSEATLNAGLLGSYGVPLLLITGDRT
                     TVEGVRHQMP"
     gene            complement(508..>1020)
                     /locus_tag="VFU90_14905"
     CDS             complement(508..>1020)
                     /locus_tag="VFU90_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5481131.1"
                     /translation="AKAGDAATLAHTFSPDTIYAVTVNGTHCSEALLVAGLAGTFGVP
                     VVLVTGDRSAVDEAVRAMPWAIGVPVKDAIGFSAVNSMAPAAACEAIGSAARDAMRGI
                     ARAKPFVFDPPFELGIETVNVENADFIELMPGFERIGGRAVRFRSASYPELLQAFVAA
                     TRLGAAANRP"
BASE COUNT          164 a          356 c          342 g          158 t
ORIGIN      
        1 acggcatctg gtgccgcacc ccttcgacgg tcgtgcggtc gccggtgatc agcagcagcg
       61 ggacgccgta gctgcccagc aggcccgcgt tgagggtcgc ttcgctgcag cggatcccgt
      121 tgacccgaac ctcataaatc acggacggcg tataagtgtg gcaaagaacg gcgtcggctt
      181 cgccggctcc gccgtgataa ccgatgaaga acgcgccgcc gaagctcgcg tcggcgtcct
      241 ggaccatcga gaacggcttg cggttgccga agagcacccg cacgtcgtgc ggcagctcgt
      301 cgaaaagcag gttgcgcatc gggccgtgac tgtcgttgac gagcacttcg ccggcgccgg
      361 cggcgcgggc gccccgaatc gcgctctcga cctcctgggt gtagtagcgc cggtagatgg
      421 cgtactccgg atgcggcgtg cgcgcgtcga cctgttccca tccgcacacg ccggcgacgc
      481 cctccatgtc gctcgagatg tagagcttca cggccggttg gcggccgccc cgaggcgcgt
      541 ggcggcgacg aatgcttgca gcagctcggg atagctagcc gatcgaaacc gcacggcgcg
      601 tccgccgatg cgctcgaaac ccggcatcag ctcgatgaaa tccgcgttct ccacgttcac
      661 ggtctcgatg cccaactcga acggagggtc gaagacgaac ggcttggcgc gcgcgatccc
      721 gcgcatcgcg tcgcgcgcgg ccgagccgat cgcctcgcag gccgctgcgg gcgccatcga
      781 attgaccgcc gagaatccga tcgcatcctt gaccggcacg ccgatcgccc acggcatcgc
      841 gcgaacggct tcgtcaaccg cgctgcggtc gccggtcacg agcaccaccg gtacgccgaa
      901 cgtgccggcc aacccggcca ccagcagcgc ttcgctgcag tgcgttccgt tgacggtgac
      961 ggcgtagatc gtgtcggggc taaacgtgtg cgcgagcgtc gccgcgtcgc cggccttggc
//
LOCUS       DASUCJ010001273         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_212573, whole genome shotgun sequence.
ACCESSION   DASUCJ010001273 DASUCJ010000000
VERSION     DASUCJ010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212573"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VFU90_14910"
     CDS             <1..1111
                     /locus_tag="VFU90_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5481132.1"
                     /translation="LLPPGVEFTGEGSPLAQIASFVETACPQCGGPARRETDTMDTFF
                     ESSWYYLRYLSPHDERAPWEIADANVWMNVDQYIGGAEHTVLHLLYSRFFYKFFRDRG
                     WVSGRDEPFERLFHQGMVLNGGEKMSKSRGNVIGIDEAAESSGIDAMRLFLLYVTPPE
                     DTGEWNEEGISGRVRFVNRVWRACEPLFAAARATGVRTLPAADTAPEKALLRAVHSAA
                     QSAVDETLTRRFHYNATIARLDELVNALTAAARDFPESPVTRYAAHALPLLVAPFAPH
                     LAEELWEGFGHTTSVHLERYLEPDERALAVEEITLVVQVNGKVRGRIPVAPGIGEERA
                     FALATAEANVRAQIDGKTLRKRIFVPDKLLNLVVE"
     gene            1185..1790
                     /locus_tag="VFU90_14915"
     CDS             1185..1790
                     /locus_tag="VFU90_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481133.1"
                     /translation="MTMDLQRALSDLAEVRGRLARVQRFEGYSGPAAAASGVCAIAAG
                     LVQLARAPAPQSSEALHAYLAVWLTCAGVAVALNYGAVGAWLLKHRGPGTQSRFRSAA
                     LSIAPSIVLGGALSVALIDRGAFSLLPGTWFAFYAIGLFASRGVIPTSCDTVTAAFTA
                     LALAFLVTPLSALALSWWVMPLGFGFGQLAIGVLLWRSRSS"
     gene            1787..2152
                     /locus_tag="VFU90_14920"
     CDS             1787..2152
                     /locus_tag="VFU90_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5481134.1"
                     /translation="MTQPQEAPFAYPGLERIFHERGRLAICTCLIAHADGLSFTELQA
                     ACDLTDGNLSRHVHALAQVGIVTTEKRRGAGRPLTVYRITKRGRQRFLAYVDELESVV
                     RDVHERSQAPLPSGKLATT"
BASE COUNT          371 a          765 c          758 g          405 t
ORIGIN      
        1 gctgctgccg cccggcgtag aatttaccgg cgaaggttcg ccgctcgccc agattgcgtc
       61 gttcgtggag acggcgtgtc cgcaatgcgg gggcccggcg cgacgggaaa ccgatacgat
      121 ggacaccttc ttcgagtcgt cctggtatta tttgcggtac ttgagcccgc acgacgagcg
      181 cgcgccctgg gaaatcgccg acgcgaacgt ttggatgaac gtcgatcagt acatcggcgg
      241 tgccgaacat accgtgctcc acctgctgta ctcgcgattc ttctacaaat tcttccgcga
      301 ccgcggttgg gtgagcggcc gcgacgagcc gttcgagcgg ctcttccatc aaggcatggt
      361 actcaacggc ggcgagaaaa tgtcgaagtc gcgcggcaac gttatcggta tcgacgaagc
      421 cgcggagagc agcggaatcg acgcgatgcg gctcttcctg ctctacgtca cgccgccgga
      481 ggacaccggc gagtggaacg aggagggaat tagcgggcgc gtgcgcttcg tcaatcgcgt
      541 ttggcgggcg tgtgagccgt tgttcgccgc cgcccgcgct accggcgtcc ggacgctccc
      601 ggcggcggac accgcacccg aaaaagcgct gcttcgcgcc gtgcactcag ccgcacaatc
      661 ggcggtcgac gagacgctca cgcggcggtt ccactacaac gccacgatcg cgcggctcga
      721 cgaactcgtc aacgcgctca cggcggcggc acgcgatttt ccggaatcgc cggtgactcg
      781 ctacgccgcg catgcgctac cgctgctggt cgcgccgttc gcgccgcatc tcgccgagga
      841 gctgtgggag ggcttcggtc acaccacctc ggtgcatctg gaacgctacc tcgaacccga
      901 cgagcgggcg ctggcggtcg aggagatcac gctggtcgtg caagtcaacg gcaaagtgcg
      961 cggacgaatc ccggtggcgc cggggatcgg cgaagagcgc gcgttcgcac tcgcgaccgc
     1021 cgaagccaac gtacgcgcgc agatcgacgg taagacgctt cgaaagcgga tcttcgtacc
     1081 cgacaagctc ttgaacctgg tcgtcgagta aagccggcgg ccttcgccgg ccgcaccggc
     1141 cgcggtctac ccttgtcagg ctcattactt tgtgatacaa agtaatgacg atggatttgc
     1201 aacgcgcgct ctccgacttg gcggaggttc gcgggcggct cgcacgcgtg cagcggttcg
     1261 agggctattc cggcccggct gccgccgcca gcggagtgtg cgcgatcgcc gcaggcctcg
     1321 ttcagctggc gcgcgcaccc gcgccgcaaa gctccgaagc gctgcacgcc taccttgccg
     1381 tctggcttac gtgcgcgggc gtcgcggtgg cgctaaacta cggagcggtg ggagcctggc
     1441 tgctcaaaca tcgcggcccg ggaacgcaaa gccgctttcg ctcggccgcc ctttcgatcg
     1501 cgccgagcat cgtcctcggc ggcgcgctca gcgtcgccct gatcgatcgc ggtgcgtttt
     1561 cgctgcttcc gggaacgtgg ttcgcgttct atgcgatcgg gttgtttgcg tcgcgcggcg
     1621 tcattccaac ttcttgcgac accgtaacgg cggcgtttac ggcgttggcg ctcgcctttc
     1681 tcgtgacgcc gctctcggca ctggcactgt cgtggtgggt gatgccgctc ggtttcggct
     1741 tcggccagct cgcgatcggc gttctcctgt ggaggagccg cagctcgtga cgcagccgca
     1801 agaggcgccg ttcgcctatc ccggtctcga acgaatcttc cacgaacgcg gccggctcgc
     1861 aatttgcacc tgtctgatcg cgcacgccga cggattgagc ttcaccgagc tccaggcggc
     1921 ctgcgatctc accgacggaa acctcagccg tcacgttcac gcactcgccc aagtcggcat
     1981 cgtaacgacc gaaaaacgcc gcggagcggg ccggccgcta accgtctatc gcatcaccaa
     2041 acgcggccgc cagcgatttc tcgcctacgt cgacgagttg gaatcggtcg tgcgcgacgt
     2101 gcacgaacgt tcgcaggctc cgctgccgag cggaaaactg gcgaccacgt gacgcccaag
     2161 cacgtcgcaa tcatcatgga cggtaatcgc cggtgggcgc gcgagcgcgg gctgccgatc
     2221 gcctgcgggt atcgccgcgg cgttaccgcg ctgcggcgcg tgttgcgcgc cgccgcgcag
     2281 gcgggcgtgg gagtcgtca
//
LOCUS       DASUCJ010001274         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1373669, whole genome shotgun sequence.
ACCESSION   DASUCJ010001274 DASUCJ010000000
VERSION     DASUCJ010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373669"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1109
                     /locus_tag="VFU90_14925"
     CDS             168..1109
                     /locus_tag="VFU90_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU5481135.1"
                     /translation="MIRALAAAGFAAACIFVQGLALAAGSNGVATGPAVSVPGIPAQT
                     VIHPGDQIGVLVYGDQTLTQNVMVLPDGTIEYPLVGRVMVAGKTPSQAAEALSVRLKP
                     YVRHPVITISIVQLGQPDVLVLGEVKQPGKYQLRSDAKLTDAIAAAGGIADTNGAFPD
                     ARIADANGKVTVVSLQSLLQRGDTSLDVHLSEGSVVYVPGPIPFTVDVSGAVDHPGDV
                     QLHEGDRLSMAIAKAGNSQNSQADLNHIRLIRTAPDGTQTTTEINLYNAIKGGDVAAD
                     VPLQKGDVVFVPQAAKHSDLFSGLGGGLLYLLTTLIP"
     gene            complement(1120..1572)
                     /locus_tag="VFU90_14930"
     CDS             complement(1120..1572)
                     /locus_tag="VFU90_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481136.1"
                     /translation="MKLLPVVLTAALLAPGAAAAQQPQTGGSKILVTECNPHEHTAAE
                     AHPWIDPYGNPHYTTTNFPAWDAFLTIGYKNQAPSAATEVDFGLTLRGSLVAVAKDVG
                     TFSPGVAIDHEFVVSREIFPLDGMPYCTVLRVKYADGTVWQNPSPPEP"
     gene            1656..>1913
                     /locus_tag="VFU90_14935"
     CDS             1656..>1913
                     /locus_tag="VFU90_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481137.1"
                     /translation="MPVDVMPRGGEKLFLGTFPPRQCGIATYTRDLIAAVDRATGGFS
                     EVVAVDDSEPGAGYAYSGRVVKRLRQHVRASYEEVARYVNEH"
BASE COUNT          332 a          634 c          616 g          331 t
ORIGIN      
        1 gccgcccgca tccgggcggc cttcggggtc cactcgcgcg ccgcgctcat cgcgtccgcg
       61 cggcacgcag gctgagccgg gcggcccccg ggtaaaggac ttccgcacca acgccgcagc
      121 ggcgggcgga tgttggcgaa cgggccggcg cgtcctacga tgaagccatg atccgcgccc
      181 tcgccgctgc cggtttcgcc gccgcctgca tcttcgtgca ggggctcgcg ctggcggccg
      241 gctcgaacgg cgtcgcaacc gggccggcag tttccgttcc gggcatcccg gcgcagacgg
      301 tcattcatcc gggcgaccaa atcggcgttt tggtctacgg cgatcagacc ctcacccaga
      361 acgtcatggt gcttcccgac gggacgatcg aatatccgct ggtggggcgc gtgatggttg
      421 ccgggaaaac gccgagtcag gcggccgagg ccttgtcggt gcgcctcaaa ccgtacgtcc
      481 gccacccggt catcacgatc tcgatcgtgc agctcggaca acccgacgta ctcgtactgg
      541 gtgaagtgaa gcagcccgga aagtatcagc tgcgatcgga cgccaagttg accgacgcga
      601 tcgccgccgc gggcggaatc gccgacacca acggcgcgtt ccccgacgcg cggattgccg
      661 acgccaacgg taaggtgacg gtcgtgtcgc tgcagagtct gctgcagcgc ggcgatacgt
      721 cgctcgacgt gcatctctcc gaaggctcgg tcgtgtacgt gcccggaccg attccgttta
      781 ccgtcgacgt ttccggcgcg gtcgatcatc ccggcgacgt gcagctgcac gaaggcgatc
      841 gcctctcgat ggcgattgcg aaagcgggca atagccaaaa ctcgcaggcc gatctcaacc
      901 atatccggct gattcgcacc gcgcccgacg gtacgcagac tacgaccgaa atcaatctct
      961 acaatgcgat caagggcggc gacgttgcgg ccgacgttcc gctgcaaaaa ggcgacgtcg
     1021 tgttcgtgcc gcaggcggcc aagcattccg acctgttcag cggtctcggc ggcgggctgc
     1081 tctatctgct gacgacgctc atcccgtaat cgtcttcgct taaggctccg gcggagacgg
     1141 attctgccag accgttccgt cggcgtactt cacgcgtaag acggtgcagt agggcattcc
     1201 gtccagcgga aagatctcgc ggctcacgac gaactcgtgg tcgatcgcga cgccgggcga
     1261 aaacgtgccg acgtctttgg cgaccgccac cagcgaaccg cgcagcgtca gtccgaaatc
     1321 gacctccgtc gcggccgacg gcgcttgatt cttgtagccg atcgtcaaga acgcatccca
     1381 agcggggaag ttcgtcgtag tgtaatgtgg atttccatat gggtcgatcc aaggatgcgc
     1441 ttcggcggcg gtgtgctcgt gcgggttgca ctcggtaacg agaattttcg agcccccggt
     1501 ctgcggctgc tgggccgccg cggcgccggg cgccagcagc gcggccgtta ggacgaccgg
     1561 aagaagcttc atgccgatca gcttagcgca cggctaagaa gaagtccgga agtgcccttc
     1621 ctcacctctt cccggcgagc gcttactatg gcggcatgcc ggtcgacgtt atgccgcgcg
     1681 gcggcgagaa gctgttctta ggaaccttcc cgccgcgcca atgcggaatc gctacgtaca
     1741 cgcgtgacct gatcgccgcg gtcgaccgcg ccaccggcgg attcagcgag gtcgtagccg
     1801 tcgacgactc cgaacccggc gccgggtacg cgtactccgg acgcgtcgta aagcggcttc
     1861 gccagcacgt ccgcgcgtct tacgaagagg ttgcccgtta cgtcaacgaa cat
//
LOCUS       DASUCJ010001275         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_136116, whole genome shotgun sequence.
ACCESSION   DASUCJ010001275 DASUCJ010000000
VERSION     DASUCJ010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136116"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="zwf"
                     /locus_tag="VFU90_14940"
     CDS             complement(<1..1019)
                     /gene="zwf"
                     /locus_tag="VFU90_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009994655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5481138.1"
                     /translation="MSVPTGEAVNPLRDPRDRRLPRVAGPCGLVLFGVTGDLSRKKLM
                     PAIYDLANRGLLPPGFALVGFARRDWADEDFAQVVHDAVKQYARTPFREEVWDQLADG
                     FRFVPGEFDDDAAFDALAQTIHDLDRVRGTGGNHAFYLSIPPKFFPLVISQLKRSGLS
                     ATDGTAWRRVVVEKPFGHDLRSARELNDVLSEVFPPESVFRIDHYLGKETVQNILALR
                     FANTLFEPLWNRSYVDHVQITMAEDIGIGGRAGYYDGIGAARDVIQNHLLQLLALTAM
                     EEPVSFDAQSLRMEKTKVLSAVRIPTDLAAHTARGQYSGGWQGGEKVCGYLEEQGIPP
                     TSTTET"
BASE COUNT          200 a          371 c          394 g          199 t
ORIGIN      
        1 gtctcggtgg tggacgtcgg cggaattccc tgttcctcaa ggtatccgca aactttctcg
       61 ccgccctgcc aaccgccgct gtattgcccg cgcgcggtgt gcgcggcgag gtcggtcgga
      121 atccgcaccg cggagagcac cttcgtcttt tccatccgaa gcgactgcgc gtcgaagctc
      181 accggttctt ccatcgcggt gagggcgagg agttgcagca ggtggttctg gatgacgtcg
      241 cgagcggcgc cgatgccgtc gtaatagccg gcgcggccgc cgatgccgat gtcttcggcc
      301 atcgttatct gcacatggtc gacatacgac cgattccaca gcggctcgaa gagcgtgttg
      361 gcgaagcgca aggccaggat gttctgaacg gtttccttgc cgaggtagtg gtcgatgcga
      421 aacaccgatt caggcggaaa aacctcgctt agcacgtcgt tgagctcgcg cgcgctgcgc
      481 agatcgtgcc cgaatggttt ttcgacgacc acgcgccgcc acgccgtgcc atcggtcgcc
      541 gacaacccgg agcgcttcag ctgcgagatg acgagcggga agaatttcgg cggaatcgac
      601 agatagaagg cgtggttacc gccggtgccg cgcacccggt cgaggtcgtg aatggtctgc
      661 gccaacgcgt cgaacgctgc atcgtcgtcg aattcgcctg gcacaaagcg aaaaccgtcg
      721 gcgagctggt cccacacctc ctcacggaac ggagtgcgcg cgtattgctt gacggcgtcg
      781 tgcacgacct gcgcgaagtc ttcgtccgcc cagtcgcggc gggcgaagcc gacgagcgcg
      841 aagccaggcg gtagcaggcc gcggttggcg aggtcgtaaa tcgccggcat caacttcttg
      901 cgcgacaggt cgccggtgac gccgaagagc accaacccgc acggtccggc gacgcggggc
      961 agtcgccggt cgcgcgggtc gcgtagcggg ttgaccgcct ccccggtcgg cacgctcatc
     1021 gcacgtgacc gaccgaaccg gcggcgtcga gcaactgctg gatgccgatc gtgcggtctt
     1081 tcaaatgcaa ccggatcgcg ggccgagcgc gctgttgcag cgcgcgaagg tcgccgagcg
     1141 cctgcgccat ctggagccgg gcga
//
LOCUS       DASUCJ010001276         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_242444, whole genome shotgun sequence.
ACCESSION   DASUCJ010001276 DASUCJ010000000
VERSION     DASUCJ010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242444"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..633
                     /locus_tag="VFU90_14945"
     CDS             46..633
                     /locus_tag="VFU90_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5481139.1"
                     /translation="MPPSSPPGQPSIRPPHPLALALIERLRPGSRVLEFCTGSGRNLT
                     ALRAAGFDAAAVADEAAAGFDLSSFPAGFDAILSTHGLLHGTTADIAARVAALGALLS
                     PGGLFYAVFGSTGDARYGRGERIAPATFAPLDGDERGVPHTYFTRSALSELLEPHLAI
                     DSLDERAADAIAGSWAHPTQPLRGAVHWFVTAHRL"
BASE COUNT          150 a          389 c          327 g          180 t
ORIGIN      
        1 ggtagtctcc gccgacgacg ataatcaacg tctcggacgc catagatgcc gccttcttcg
       61 ccacccggcc agccctccat ccggccgccc catccgctgg cgctcgcgct gatcgagcgc
      121 ctgcgtcccg ggagccgcgt actcgaattc tgtaccggca gcggccgcaa cctcaccgca
      181 ctgcgcgccg cggggttcga cgccgcggca gtcgccgacg aggccgccgc cggcttcgac
      241 ctgtcgtctt tcccggccgg cttcgacgcg attttatcca cgcacgggtt actgcacggc
      301 accacggccg atatcgcggc gcgcgttgcc gcgctcggcg cgctgctgag ccccggcggc
      361 ctgttctacg cggtcttcgg cagtaccggc gacgcacgct acggccgcgg agaacggatc
      421 gctcccgcga cgttcgcacc gctcgacggt gacgagcgcg gcgtccccca cacgtacttc
      481 acgcgctccg cgttgtccga attgctggag ccgcatctgg cgatcgattc gttggacgaa
      541 cgcgccgccg acgcgatcgc cggatcgtgg gcgcatccca cgcaaccgct gcgcggcgcc
      601 gtccactggt tcgtcaccgc ccaccgcctg taacgctcgt ccgcggtagt gtccggttcc
      661 gggacgctat gcagactgtg tcatcctgag cggaggcgcg aagcgccgca gtcgaaggac
      721 gcgctcgttg taacgcacga cggtggggac gccgtcgctc ggacgttcgt cgctcgcgtc
      781 gatgactcgc tcctactcgt gcgctttttc gcaactcaaa cggtgtcatc ctgagcggag
      841 ctcgcgtgag cgagcgcagt cgaaggactg gcttttgcga aaaagtcgca cgcctcgctc
      901 acggcatccc caccgtcgcg ctaccgtaga gcgtccttcg actacggcgc tccgcgcctc
      961 cgctcaggat gacacggctg aggctccgcg gctccgctca ggatgacacg gctgaggctc
     1021 cgcggctccg ctcaggatga cacgtc
//
LOCUS       DASUCJ010001277         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1221151, whole genome shotgun sequence.
ACCESSION   DASUCJ010001277 DASUCJ010000000
VERSION     DASUCJ010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221151"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..208
                     /gene="cspD"
                     /locus_tag="VFU90_14950"
     CDS             5..208
                     /gene="cspD"
                     /locus_tag="VFU90_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HEU5481140.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGEDLFAHFSAINMQGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAV"
     gene            364..813
                     /locus_tag="VFU90_14955"
     CDS             364..813
                     /locus_tag="VFU90_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481141.1"
                     /translation="MAGTPSDNDTGALEFEKVSIADARKAIEDGGPAGHASTASRTES
                     WDAKRHASPDEGLSADAAAWLAELPAGIRPSQLALRYARVANRISETWKAPLHCLRLL
                     DQLMTDERGGRRGFPLQVATELATLRDYYYRLHHRGKEAWEHVEMGR"
     gene            complement(824..>2250)
                     /locus_tag="VFU90_14960"
     CDS             complement(824..>2250)
                     /locus_tag="VFU90_14960"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481142.1"
                     /translation="YEVTPAIAGAYQTLPRPGTTYARGLPRNQPDTGFPDDLPNGPYR
                     ITRYVPYTAIVGDPLHRFFQMWQQLDGGKRDLFTWVAMTSGYGAIERGNPAAGTGQGG
                     VAMGYYDMAAGDAPYFAKLARTWALADNDHQPIMGGTGANYFALATGDVAAFQDDGRL
                     ARPSENRIENPEPVAGTANWYTQPGRAGSYTGCADPSQPGVRAIRDYLAKLPYKAWRD
                     GNCAPGAYYLVNNYPPAYTFDGALRWKTLRSPVLPPQTMPNIGSELAAAGVSWKWYSG
                     GRIGDGIDKARYCPICDPLTRFTSVMTTELRARLVGLDAFERDLADAATFPAVSFVVP
                     PNPDSGHPAFSTVAEFETFVRGVVTKVQARPELWKSTAILVTTDESGGSWDSGYVQIL
                     DFFGDGTRIPLIAVSPWAKKGYVDHTYYDHVSILKFIEKNWRLAPLSARSRDNLPNPV
                     PSAADPYVPANRPAIGDLTGLFAF"
BASE COUNT          381 a          746 c          767 g          356 t
ORIGIN      
        1 agcaatggca accggaactg taaagtggtt caacgacgcg aagggcttcg ggttcatcac
       61 gccggatggc ggcggtgaag acctgttcgc tcacttctcg gcgatcaaca tgcagggctt
      121 caagacgctc aaggaaggcc aaaaggtcag cttcgaagtg acccagggtc ccaagggcaa
      181 gcaggcttcg aacatccagg ccgtctgagc cccggattcg attcacgaaa ggccgggcgc
      241 tgcccggcct ttttttgcgt cctcgtgccg ctggtcggaa ggcgacgacc gcaagtgccg
      301 cgcgacgcgt atcgctgtcc cgaccgcggt agcatcgtca gagtcgcgaa acggagatca
      361 cccatggcag gcacgccgag cgacaacgat accggcgcgt tggagttcga gaaagtctcg
      421 atcgccgacg cgcgcaaagc catcgaggac ggcggccccg cggggcacgc gtccaccgcg
      481 agcaggaccg agagctggga cgcgaagcgc cacgcgtcgc ctgacgaggg cttgtcggcc
      541 gacgcagcgg cgtggctcgc cgagctgccg gccggcatcc ggccgagcca gctcgcgctg
      601 cgctacgcac gagtcgccaa ccgcatcagc gagacgtgga aggcgccgct gcattgtctg
      661 cgcctcctcg accagttgat gaccgacgag cgcggcgggc gccgcggctt tccgctgcag
      721 gtcgcaaccg agctcgcgac gctgcgcgac tactactatc ggctgcacca ccgcggcaag
      781 gaagcgtggg agcacgtcga gatggggcgt tgattgggtg gggtcagaag gcgaacagcc
      841 ccgtcaggtc gccgatcgcc ggccggttcg ccggcacgta cggatcggcg gcacttggca
      901 ccggattcgg cagattgtcg cgacttcgcg ctgacagcgg cgcgaggcgc cagttcttct
      961 cgatgaattt caggatcgac acgtgatcgt agtacgtgtg gtcgacgtag cccttcttcg
     1021 cccacggtga caccgcgatc agcggaatgc gcgtgccgtc gccgaagaag tcgaggatct
     1081 ggacgtagcc gctgtcccag cttcctccgc tctcgtcggt agtgacgagg atcgccgtcg
     1141 acttccacag ctcgggccgc gcctgcacct tcgtcaccac accgcgcacg aacgtctcga
     1201 actcggcgac cgtcgagaac gccggatggc ccgagtcggg attcggcggc acgacgaacg
     1261 acaccgccgg aaacgtcgcc gcatcggcga ggtcgcgctc gaacgcgtcg aggccgacga
     1321 gcctcgcgcg aagctcggtc gtcatcaccg acgtgaatcg cgtcagcgga tcgcagatcg
     1381 ggcaataacg ggccttgtcg atgccgtcgc cgatgcggcc gccgctatac cacttccacg
     1441 aaacgcccgc cgccgcgagc tcgctgccga tattcggcat cgtctgcggc ggcagcacgg
     1501 ggctcctcag cgtcttccag cgcagcgcac cgtcgaacgt gtaggcaggc ggatagttgt
     1561 tgacgaggta atacgctccc ggcgcgcagt tgccgtcgcg ccaggccttg tacgggagct
     1621 tcgcaagata gtcgcggatc gcgcgcacgc cgggctgcga cgggtcggcg cagccggtgt
     1681 acgagccggc gcgaccgggc tgcgtgtacc agttggcggt gcccgcgacc ggctccggat
     1741 tctcgatacg attctcggac ggccgcgcga gcctgccgtc gtcctgaaac gctgcgacgt
     1801 cgccggtcgc gagtgcgaag tagttggcgc cggtcccacc catgatcggc tggtgatcgt
     1861 tgtcggcaag cgcccaggtc cgcgcgagct tcgcgaaata cggcgcgtcg ccggccgcca
     1921 tgtcgtagta gcccatcgcg acgccgccct gcccggttcc ggctgccgga ttgccgcgct
     1981 cgatcgcgcc gtacccggag gtcatcgcca cccaggtgaa gaggtcgcgc tttccgccgt
     2041 cgagctgctg ccacatctgg aagaagcggt gcagcggatc gccgacgatc gccgtgtacg
     2101 gaacgtagcg tgtgatccgg tacggcccgt tcggcagatc gtcgggaaag cccgtgtcgg
     2161 gctggttgcg cggcaaaccg cgcgcgtagg tggtccccgg ccgcggcagc gtctggtacg
     2221 cgccggcgat tgccggcgtc acttcgtaac
//
LOCUS       DASUCJ010001278         1285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1480705, whole genome shotgun sequence.
ACCESSION   DASUCJ010001278 DASUCJ010000000
VERSION     DASUCJ010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480705"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..>1285
                     /locus_tag="VFU90_14965"
     CDS             257..>1285
                     /locus_tag="VFU90_14965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5481143.1"
                     /translation="MSLLRPAVFAAALISAPAAAQQTPPAKPMPAPPSASAHAPEIPD
                     IAYTRFVLGNGLTVLVHEDHKAPIVAVNLWYHVGSKNEVIGKTGFAHLFEHLMFAGSE
                     NHNDRYIPAAEALGATDLNGTTNEDRTNYFETVPVSALDRTLFLESDRMGHLLGAIDS
                     ARLALQRGVVQNEKRQGENEPYGKTEVIIPENTYPAGHPYSHSVIGSMDDLDAASLED
                     VKNWFRTYYGPANVTLSIAGDITVEAARQKAQQYFGDIPPGPPITKQSTWIAKMTGVH
                     RQIMQDRVPQARVYQVWNVPEFGAPDATYLGLVASILGSGKASRLYKRLVYRDRTATD
                     VFAYLNARE"
BASE COUNT          232 a          491 c          372 g          190 t
ORIGIN      
        1 accctgctcg cggtcaccgc gctcctgctc gttgcctccg tcgccgccgc gctcatcccc
       61 tgctggcggg ccgcacgcgt ccacccggcg cgcagcctcg ccgagtagcc gcgtcggcgg
      121 acgccggccg cgtctgttcc cgtatcggta gaatcgatgc gttgggcgaa cgcgcccctt
      181 gagaaacggg ccggcggcgg ccatgatcgc tggcggccaa ccacgcgtcg cgcttcctcc
      241 accccgaggc gggtccatgt ccctgctccg tccagccgtc ttcgccgccg cgctgatcag
      301 cgcgcccgcc gccgcgcagc agacgccgcc ggccaagccg atgcccgcgc cgccatccgc
      361 atcggcgcac gcgccggaaa tccccgacat cgcgtacacc aggttcgtcc tcggcaacgg
      421 cctaacggtg ctcgtgcacg aagaccacaa ggcgccgatc gtcgccgtca acctgtggta
      481 ccacgtcggc tcgaaaaacg aagtcatcgg caagacgggc ttcgcgcatc tcttcgaaca
      541 cctcatgttc gcggggagcg agaaccacaa cgaccgctac atccccgcgg ccgaagccct
      601 cggcgcgacg gacctcaacg gcacgaccaa cgaagaccgc accaactact tcgagaccgt
      661 gcccgtctcg gcgctcgatc gcacgctgtt cctggaatcg gaccgcatgg ggcacttgtt
      721 aggcgcgatc gattccgccc ggctcgccct ccagcgcggc gtcgtccaga acgagaagcg
      781 ccagggcgaa aacgagccgt acgggaaaac cgaggtcatc atcccggaga acacctaccc
      841 cgccggccat ccgtattccc actcggtgat cggctcgatg gatgatctcg acgccgcgtc
      901 gctggaagat gtcaagaatt ggttccgcac ctactatggt ccggccaacg tcaccctctc
      961 gatcgccggc gacatcacgg tcgaagccgc gcgccagaaa gcacaacagt atttcggcga
     1021 cattccgccc ggcccaccga tcaccaaaca gagcacgtgg atcgccaaga tgaccggcgt
     1081 gcaccgccag atcatgcagg accgcgttcc gcaggcgcgc gtgtatcagg tgtggaacgt
     1141 gcccgaattc ggcgcccccg acgcgaccta cctcggcctc gtcgccagca ttctcggctc
     1201 gggcaaagcg tcccgattgt acaagcgcct cgtctaccgc gaccgcacgg ccaccgacgt
     1261 cttcgcctac ctgaacgcac gcgag
//
LOCUS       DASUCJ010001279         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_915368, whole genome shotgun sequence.
ACCESSION   DASUCJ010001279 DASUCJ010000000
VERSION     DASUCJ010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915368"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFU90_14970"
     CDS             complement(<1..519)
                     /locus_tag="VFU90_14970"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEU5481144.1"
                     /translation="MNGSPHEAPRFAHSVRAPTWDRGAFVAKKLVRSPYPRLVGRAAL
                     GVAQVQVAAWDFALRRVRATQESTLLSLLKLNEETEFGRAHSFSRIRDYAGFARAVPV
                     GDYDSFSPFIDRMRAGESNVLVPEFVQHFGNSSGSSNHGKSKFLPITDRQVRFQQRAG
                     TDAVMRYLHWSGD"
BASE COUNT          252 a          451 c          487 g          214 t
ORIGIN      
        1 gtcgcccgac caatgcaggt agcgcatgac ggcgtcggtg ccggcgcgct gctggaatcg
       61 tacctgacga tcggtgatgg gcaggaactt ggacttgccg tgattcgagc tccccgacga
      121 gttgccgaag tgctgtacga actcgggaac gagcacgttc gactcgcccg cccgcatccg
      181 gtcgatgaag ggcgagaacg aatcgtaatc gccgacgggg accgcgcgcg cgaaccctgc
      241 gtaatcacgg atgcgcgaaa atgaatgcgc acgaccgaac tcggtctctt cgttcagctt
      301 gagaagagag aggagcgtcg actcttgggt ggcgcggacg cggcgaaggg cgaagtccca
      361 cgcggcgacc tgcacctggg cgacgccgag cgcggcccgg ccgacgaggc gcggataggg
      421 ggagcgaacc agtttcttgg cgacgaaggc gccgcgatcc cacgtgggtg cgcggacgct
      481 gtgcgcgaac cggggcgctt cgtggggcga tccgttcatc tggctcgacg tcatagtggc
      541 tggtgcggcc gatcctcaag ggatcaagcg cgccgctggc tccgagggac ggtggagccc
      601 gccgccggca ccgaagccga gggcgaaggc tcgaaaacgt gctgcaacga caatacgacc
      661 tcagatgcct gttggagcgg ggaagagagg ggggtaggga agccgaggcg gtcggtgtcg
      721 cgcgtgccgg gtgcgaagcg cgcaccgccc gcactcgcgc cactgcacag aacgccaagg
      781 ggatactcgg acgtcacatg gatcgccacc gatttgtcgc gcgcgctcgc acctcgcgag
      841 ctacggaggg aactcgccct cgatcgacgg gagcatccac gaagtgactt tcgatcaaac
      901 gccgtctgcg ctgcaggtcg cgcgcgtcgc gcagctcgca acgcgggcga tggagaaacg
      961 gggcaacgac gaaacggcat gcatcgctcc ggaggcgcgg agcggcgcca ccttacggac
     1021 ggtcggccac aagacaaagt cgtcggttcg tctcggttgg tttttgttct tctcgatttt
     1081 ttcattcacg gtcgccgtcc cggtccgccg cgctcgagcc gacacgccta gcggcgcttc
     1141 cccgacttcg ccgtctcccg tcgtgcccgt cacgactccg atcgtaccgc ccgcccttcc
     1201 cgcgcagagc ggccccgcgg tcacaggctc ggacgagcaa agccgccaaa ccggcgtctc
     1261 gaaaggcgtt tcgcctccgg gcgtggacgg gggccgaacc aagaaggccg attcgccgcg
     1321 gcgttacgag ctcgctggct tccccatcat cggcggaaac agtgacatcg gattccagtt
     1381 cggcggtgcc gcgacgctga cgcg
//
LOCUS       DASUCJ010001280         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1006903, whole genome shotgun sequence.
ACCESSION   DASUCJ010001280 DASUCJ010000000
VERSION     DASUCJ010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006903"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VFU90_14975"
     CDS             complement(<1..1368)
                     /locus_tag="VFU90_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5481145.1"
                     /translation="MTLAGVGPSASAGQGTAAPWLFAAMKARQGAPALAWGGTVLSYG
                     DLVFRTEAFERELDARGIGAGSVVLVEGSFSPNAVALLLALIRKNAIAVPLTPVARVH
                     RDRFVEIAEASSAFEFDEDDHYKFVPIGRPVTNALTKKLIARGHPGLVIFSSGSTGNP
                     KAILHDMHAMLEKFNKPGTPKSILTFLLFDHIGGMDTLFHSLASGGTMVTTKTRDPGV
                     VCEAIAAHRVHTLPASPTFLNLLMVAGVAQNHDLSSLRVIAYGTEAMPPNLLKRLQEA
                     FPNVKFVQTYGMSELGVLRTRSREGDSAWIKFTGEGFETKIVDDVLWVRTPTAMMGYL
                     NAPDLFDEEGWLNTEDAVEVRGEYLRILGRVTDLINVGGQKVYPAEVENVILQMDNVR
                     DVAVYGEKSPLTGHFLAARVNLINPEPFDAFKKRLRTFCRERLASYQVPVRIEITDAD
                     QFGIRM"
BASE COUNT          255 a          492 c          500 g          280 t
ORIGIN      
        1 catgcggatc ccgaactggt cggcatccgt gatctcgatg cgaacgggga cctggtagct
       61 cgcgagccgc tcgcggcaga acgtgcgcag ccgcttcttg aacgcatcga acggctcggg
      121 gttgatcaag ttgacgcgtg ccgcgagaaa gtgccccgtg agcgggctct tctcgccgta
      181 gacggcgacg tcgcggacgt tgtccatctg gaggatcacg ttctcgacct cggcggggta
      241 caccttctgc ccgccgacgt tgatcaagtc ggtgactcgg ccgaggatcc ggagatactc
      301 gccgcggact tccacggcgt cttcggtgtt gagccacccc tcttcgtcga acaagtccgg
      361 cgcgttcagg tagcccatca tcgccgtggg cgtccgcacc cagaggacgt catcgacgat
      421 cttggtctcg aacccctcac cggtgaactt gatccacgcg gaatcgccct cgcgcgatcg
      481 cgtacgcaat acgccgagct cggacatccc gtacgtctgg acgaacttga cgttcggaaa
      541 cgcctcctgc agccgcttga gcaagttcgg cggcatcgct tccgtgccgt aggcaatcac
      601 gcggagcgac gaaaggtcgt ggttttgggc cacccccgcc accatcagca ggttgaggaa
      661 cgtcggcgac gccggcaacg tgtgcacgcg gtgggcggcg atggcttcgc agaccactcc
      721 ggggtcgcgc gtcttcgtcg tgaccatcgt gccgccgctc gcgagcgagt gaaacagcgt
      781 gtccatcccg ccgatgtgat cgaacaggag gaacgtgagg atgctcttcg gcgtgcccgg
      841 cttgttgaac ttctcgagca tcgcgtgcat gtcgtgcagg atggccttcg ggttgcccgt
      901 cgatccggac gagaagatca cgagccccgg atgtccgcgc gcgatgagct ttttcgtcag
      961 tgcgttcgtc accggccggc cgatgggcac gaacttgtag tggtcgtctt cgtcgaactc
     1021 gaacgcggag gaggcctcgg cgatctcgac gaagcgatcg cggtgcacgc gggcgacggg
     1081 cgtgagaggc acggcgatcg cgttcttgcg aatcagcgcg agcagcagcg caacggcgtt
     1141 gggcgagaag ctcccttcga caaggacgac gctgccggcg ccgatgccgc gagcgtcgag
     1201 ctcccgttcg aacgcctcgg tgcggaacac gaggtcgccg tacgagagga cggtgccgcc
     1261 ccatgcgagc gcgggcgcgc cctgccgtgc cttcatcgcg gcgaagagcc acggtgccgc
     1321 cgtcccctgc cccgcgctcg ccgacggccc cacccccgca agcgtcatgt aatgccgccc
     1381 aaatagatca cttggccggt gatgttgtcg ctcgccggac tgatgaagaa gtcgcagacg
     1441 ttgatgcagt cttcgttcgt gccgagacgc ttgatcgcga ggttgttcac gacgccgtcg
     1501 attttgtcct gcggcacgcc tttgatc
//
LOCUS       DASUCJ010001281         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1037645, whole genome shotgun sequence.
ACCESSION   DASUCJ010001281 DASUCJ010000000
VERSION     DASUCJ010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037645"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFU90_14980"
     CDS             <1..548
                     /locus_tag="VFU90_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-aminobenzoate-CoA ligase"
                     /protein_id="HEU5481146.1"
                     /translation="RPVPGYVAEVHDDEGRRVQNGTVGRLAVKGPTGCKYLDDERQRL
                     YVQDGWNYPGDAYRMDDDGYLWYVARTDDMIISAGYNISGPEVEEVLIAHVEVREAAV
                     VAKPHPEKQTNIVKAFVVLESIAAADDAKAEELLDFCKARIAPFKAPREIEFVAELPR
                     TETGKVQRYKLRQISKEAASS"
     gene            complement(545..958)
                     /locus_tag="VFU90_14985"
     CDS             complement(545..958)
                     /locus_tag="VFU90_14985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5481147.1"
                     /translation="MSEAINPEGWPRPSGYSNGMAARGRFLAISGQIGWNERGELAGR
                     FLDQAAQALRNVVAVLDAAGGEPGNLVRLTWYVVDKNEYRAALRDLGRAYREIVGEHY
                     PAMTLVQVADLLEDGARVEIEATAILPDDRPSVPR"
     gene            complement(1019..2491)
                     /locus_tag="VFU90_14990"
     CDS             complement(1019..2491)
                     /locus_tag="VFU90_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU5481148.1"
                     /translation="MSSGAIVLLALVGAVTVMGFLAARWGRPDLTRIEEWALGGRRFG
                     TLVSWFLLGGDLYTAYTFIAVPALVYGIGALGFFAVPYATIAYPVALVVLVRFWGIAR
                     ERGYVTTADFVRDRFGDRTLEIAVALTGVVAAMPYIALQLVGMKVVFAHLGGWFSAYG
                     NLPALTAAFALLAAYTYTSGLRAPAMIAFVKDTLIYVTVVAAVIVIPAKLGGWGHVFS
                     VSGAVLAARPKPASIYLAPPQYFTYATMAFGSALSLFIYPHSITSVLAARSKEVIRRN
                     AALLPVDSLLLGLLALLGYCAVAAGIHVPARDSSSIVPLLFARFFPDWFAGIAGAAVV
                     IGALVPAAIMCIGAANLFASNVFAEFSSNRPAAETRVARLLTLAMCAGALLFIFFVPV
                     PYAIDFQLLGGALMLQIFPAFVLGLWTRWFHPRALLAGWAAGLIASCAMAYAGGFGPN
                     FTIAAFGWSVTGFIALYGLAVNLAVAALATPILRKSRSHA"
     gene            complement(2484..2675)
                     /locus_tag="VFU90_14995"
     CDS             complement(2484..2675)
                     /locus_tag="VFU90_14995"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEU5481149.1"
                     /translation="MKRAVYGLLVLPFVGTLIPGLYNRAQPALFGMPFFYWYQLGWVI
                     LTAGLLGLVVYLTRDGRDV"
     gene            complement(2704..>2998)
                     /locus_tag="VFU90_15000"
     CDS             complement(2704..>2998)
                     /locus_tag="VFU90_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5481150.1"
                     /translation="GWAVWGPFIFAVVFPIVLTWPVWNYGIAQIGAARAGLFGFLVPI
                     VAGVASTAIFGARFEPHQLIGAAICIAGMILASLFGRFSVAAIWSERSLPLER"
BASE COUNT          640 a          950 c         1003 g          405 t
ORIGIN      
        1 gccggccggt gccgggttac gtcgccgagg tgcacgacga cgaagggcgc agggttcaga
       61 acggcacggt cgggcggctc gcggtcaaag gcccaacggg ctgcaagtat ctcgacgacg
      121 agcggcagcg cttgtacgta caagatgggt ggaactatcc cggcgatgcg taccggatgg
      181 acgacgacgg gtacctctgg tacgtcgccc ggacggacga catgatcatc tcggccggct
      241 ataatatttc cgggcccgaa gtagaagagg tcctgatcgc ccacgtcgag gtccgggagg
      301 cggccgtggt tgcaaagccg catcccgaaa aacaaaccaa catcgtcaag gctttcgtcg
      361 tgctggagag catcgccgcg gccgacgacg cgaaggccga ggagctgctc gatttctgca
      421 aggcgcgaat cgcgccgttc aaagccccgc gagaaatcga gttcgtcgcc gagttgccgc
      481 ggacggaaac gggtaaggtg cagcgctaca agctgcgcca aatctcgaag gaggcggctt
      541 cctcctagcg cggtaccgag ggacggtcgt ccggcaggat cgcggtcgcc tcgatttcca
      601 cccgcgcgcc gtcttcgagc aaatcggcga cttgcacgag cgtcatcgcc ggatagtgtt
      661 ccccgacgat ttcgcgatac gcgcggccga gatctcgcag cgcggcgcga tactcgttct
      721 tgtcgacgac gtaccacgtg agccgtacca ggttgccggg ttcgccgccg gcggcgtcca
      781 aaacggcgac gacgttgcgt aacgcctggg ccgcctgatc gagaaatctg ccggccagct
      841 cgccgcgttc gttccatccg atctgcccgg agatcgcgag gaaccgcccg cgcgcggcca
      901 tcccgttgga atagccactc ggacgaggcc agccctcggg gttgatcgct tcgctcatga
      961 acgggtctcc gacggcgagt gagtgcgcga gcgtttctgg gacgattagc cgattccgct
     1021 aggcatgcga ccgcgactta cgcaaaatcg gcgtcgcgag cgcagcaacc gcgagattga
     1081 ctgccagccc gtagagcgcg atgaagcccg tgacgctcca cccgaaggcg gcgatcgtga
     1141 agttgggtcc aaaaccgccg gcataggcca tcgcacagct cgcgatcagg cccgccgccc
     1201 agccggccag taacgcccgc gggtgaaacc agcgggtcca caggcccagc acgaacgcgg
     1261 ggaagatctg cagcatcagc gctccgccga ggagctgaaa gtcgatcgcg tacggcaccg
     1321 gcacgaagaa gatgaacagc agggcgccgg cgcacatcgc gagcgtgagc agccgcgcga
     1381 cccgcgtctc ggcagccggc cggttcgacg aaaactcggc gaagacgttg ctcgcaaaga
     1441 ggttggcggc gccgatgcac atgatcgccg ccgggaccag cgctccgatc acgaccgccg
     1501 cgccggcaat gccggcaaac caatcgggga agaagcgcgc gaacagcaag ggcacgatgc
     1561 tgctgctatc gcgtgcgggc acgtgaatgc cggcggcgac ggcgcagtag ccgaggagcg
     1621 cgagcagccc cagcagcagc gaatccacgg gcaacagcgc ggcgttacgc cggatgacct
     1681 ccttgctgcg ggccgccaaa acgctggtga tcgaatgcgg gtagatgaac agcgagagcg
     1741 cggagccgaa ggccatggtg gcataggtaa agtattgcgg cggggcgagg tagatcgagg
     1801 caggtttggg gcgagcggca agcacggcgc cggacaccga aaacacgtgg ccccagccgc
     1861 cgagtttagc gggaatcacg atcaccgctg caacgacggt gacgtagatg agcgtgtcct
     1921 tgacgaatgc gatcatggcg ggcgcgcgca aaccgctagt gtacgtgtag gccgccagta
     1981 acgcgaacgc tgcggtcaac gccgggagat tcccgtaggc cgaaaaccag ccgcccaagt
     2041 gggcgaagac gaccttcatg ccgacgagtt ggagggcgat gtagggcatc gccgctacga
     2101 cgccggttag cgccaccgcg atttcgaggg tgcggtcgcc gaagcggtcg cgaacgaaat
     2161 cggcggtcgt gacgtatccg cgctcgcggg cgatgcccca aaaacgtacc agcaccacga
     2221 gcgcgaccgg ataggcgatg gtcgcgtacg gaaccgcgaa gaagccgagc gcgccgatac
     2281 cgtagacgag cgccgggacc gcgatgaacg tgtaggcggt gtagaggtcg ccgccgagca
     2341 agaaccagga gaccagggtg ccgaaccggc gcccgcccag cgcccactcc tcgatgcggg
     2401 tcaggtcggg tcgcccccac cgcgcggcca gaaatcccat gaccgtcacc gcgccgacca
     2461 gcgcgagcaa aacgatcgca ccgctagaca tcgcgaccgt cacgcgtgag ataaacgacc
     2521 aggccgagca gcccggcggt gaggatcacc caaccgagct gataccaata aaagaacggc
     2581 ataccgaaca gagccggttg cgcgcgattg tacagtccgg ggatcagcgt gcctacgaac
     2641 ggcaagacta gaagtccgta gacggcgcgc ttcacggtgt cctgtctttg tcgtcggccg
     2701 gagctatctt tccaagggta acgaccgttc cgaccagatt gcggcaaccg agaaccgccc
     2761 gaacaagctg gcgaggatca tcccggcgat gcagatcgcc gcgccgatga gctggtgagg
     2821 ctcgaacctc gcgccgaaga tggccgtact cgcgaccccg gctacgattg gaacgagaaa
     2881 tccgaacagg ccggctcgcg ccgcaccgat ctgcgcgatg ccgtagttcc aaaccggcca
     2941 cgtcaagacg atcggaaaga cgacggcaaa gatgaacgga ccccagaccg cccatccc
//
LOCUS       DASUCJ010001282         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_915379, whole genome shotgun sequence.
ACCESSION   DASUCJ010001282 DASUCJ010000000
VERSION     DASUCJ010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915379"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VFU90_15005"
     CDS             complement(<1..1912)
                     /locus_tag="VFU90_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU5481151.1"
                     /translation="MSTRLRICCSVLSVLLAARAMPAQQRAGSAVDWPVYAHDAAGTK
                     YSPAARITRSNVKRLVPAWTYRTGDFSLGDGMTRDETTPLFVDGLLYVSTPFGGVRAL
                     DPETGHERWSFDSELDLSGDYGDFANRGVSTWVDPRRPAGEMCHRIIYVAPVDSRLIA
                     LDARTGAPCAGFGDHGVVRLGDVRNPEIFRGEYEITSPPAVVNGLLIVGSTVADNVRA
                     KAPSGVVRAFDARTGQLRWAWDPVAREPTASGYDSWRGPDAHETGAANAWSIISVDSA
                     RDLVFVPTGSPSPDFFGGDRLGQNLYANSVVALRASTGAMVWHFQVVHHDLWDYDVPA
                     EPVLFTWRHDGRDTPALAATTKMGHVFLLNRETGAPLFPVEERRVPASDVPGDSAWPT
                     QPFPTRPVPLVPERFQADDVFGVSAQGKAWCEKELAGVRNDGMFTPPSVRGTVIYPGN
                     IGGSNWSGIAIDPVRHLAILPLNNVMTVVQLIPRADAHARAMSGTRFDQFSPQRGTPY
                     AMRRTFLIAPDGAPCSTPPWGTLTAVDLETGRPTWSRPLGSIAALARVPGSARWGSPN
                     LGGAMVTAGGIVFAGGALDERLHAYDIETGTELWSAKLPAGVHASPMTYVTASGRQFV
                     VVAAGGHRELYAA"
BASE COUNT          386 a          811 c          742 g          356 t
ORIGIN      
        1 ttgccgcgta cagctctcgg tgtccgcccg ctgcgaccac cacgaactgt cgtccgctcg
       61 cggtgacata ggtcatcggc gacgcatgga cgccggccgg cagtttggcg gaccacagct
      121 ccgtgccggt ttcgatgtcg tatgcgtgca gccgctcgtc gagcgcgccg cccgcgaaca
      181 cgatcccacc ggcagtgacc atcgcgccgc ccaagttagg cgagccccac cgcgcgctgc
      241 ccggtacgcg cgcgagtgca gcgatgctcc ccaacggacg gctccacgtc ggccgacccg
      301 tctcgagatc caccgccgtg agggtgcccc acggcggcgt cgaacacggc gcgccgtccg
      361 gtgcgatgag aaaggtccgc cgcatcgcgt acggcgttcc gcgctgcggc gagaactgat
      421 cgaagcgcgt gcccgacatt gcgcgggcat gcgcatcggc acgcggaatc aactgcacca
      481 cggtcatcac gttgttcaac ggaaggatcg cgagatgccg caccgggtcg attgcgatcc
      541 cgctccagtt cgacccgccg atgttcccgg gatagatcac ggtgccgcgc accgatggcg
      601 gcgtgaacat cccgtcgttg cgcacaccgg caagctcctt ctcgcaccat gccttccctt
      661 gggccgacac gccgaacaca tcgtcggcct gaaatcgctc cgggactaac ggaacgggac
      721 gcgtcgggaa cggctgcgtc ggccacgcgg agtcgccggg gacgtcgctc gccggaacgc
      781 gccgctcttc gacgggaaag agcggtgcgc cggtctcgcg attcagcagg aagacgtggc
      841 ccatcttggt cgtcgcggcg agcgccggcg tatcgcgccc gtcgtggcgc caggtgaaga
      901 gcaccggctc cgcgggtacg tcgtagtccc acaggtcatg gtgcaccacc tggaagtgcc
      961 agaccatcgc gcccgtcgat gcgcgcagcg ccacgacgga attcgcgtac aggttctgtc
     1021 cgagccggtc accgccgaag aagtctggac tggggctgcc ggtgggcacg aacaccaggt
     1081 cgcgcgccga gtcgacgctg atgatcgacc acgcgttggc ggcgccggtt tcgtgcgcgt
     1141 ccgggccgcg ccacgaatcg tacccggaag ccgttggctc gcgcgcgacg ggatcccatg
     1201 cccaacgcag ttgcccggtg cgcgcatcga atgcgcgaac gacgccgctg ggcgccttgg
     1261 cgcgcacgtt gtcggcaacg gtcgagccga cgatcaacag tccattgacg acggccggcg
     1321 gcgacgtgat ttcgtattcg ccgcgaaaga tctcggggtt gcgcacatcg cctaaccgga
     1381 ccacgccgtg gtcgccgaat cccgcgcagg gggcgcccgt gcgggcgtcg agcgcgatca
     1441 gccgtgagtc gaccggcgcc acgtaaatga tgcggtggca catctcgccg gcgggccgcc
     1501 gcgggtccac ccacgtcgac acgccgcggt tggcaaagtc gccgtagtcg ccggacaggt
     1561 cgagctccga gtcgaacgac cagcgctcgt gtcccgtctc cgggtcgagc gcgcgcacgc
     1621 cgccgaacgg cgtcgatacg tagagcaggc catccacgaa cagcggcgtc gtctcatcgc
     1681 gcgtcatgcc gtcgcctaac gagaaatcgc ccgtgcgata cgtccatgcc ggaacgagcc
     1741 gcttcacgtt cgagcgcgtg atgcgcgccg ccggcgagta cttggtgccg gcggcgtcgt
     1801 gcgcatacac cggccagtcg acggccgaac ccgcgcgctg ttgcgccggc atcgcgcgcg
     1861 ccgcgagcag aaccgacaat acactgcagc agatacgcaa acgcgtgctc atgttcgaga
     1921 cctcgatgag tgctcaggcc ctcgcgagcc cgagctcgac gcgcctgatg ggttcctcga
     1981 caatgattga tccatgagcg aggagactaa ccagggtgcc tcgcccatga cattccgcac
     2041 ggcacctgtt ttcacgaacg tgcggaagac cccaaaaaaa gaaggcaact ggcgtcgctt
     2101 cgccaatcgc cttcattccc tctcctgtcg acgggtcacc ctgccgtcac ccgcaacgtc
     2161 gcgtcgtacg cgaccgttcc tgcgcgaccg acggcagtgc ggccgcaccc tcgatcttat
     2221 gcggctttct tgcccgactg ctgccaactc acctgttcgt tcagcccctc gatgagtgcc
     2281 tcgaagtgcg ccggc
//
LOCUS       DASUCJ010001283         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1358888, whole genome shotgun sequence.
ACCESSION   DASUCJ010001283 DASUCJ010000000
VERSION     DASUCJ010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358888"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1882
                     /gene="pepN"
                     /locus_tag="VFU90_15010"
     CDS             16..>1882
                     /gene="pepN"
                     /locus_tag="VFU90_15010"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEU5481152.1"
                     /translation="MDTQASNEPQATDGVVRLAEYRPPAWRVTHVELTFELGIDETVV
                     RSKLTLARDRDEPLRLDGEDIALLAARLDGRRLDDSEYSLDAAGLTVPAARDGSVLEL
                     DVRVEPARNSQLSGLYLSGAPERGFLLTQCEAQGFRRITFFPDRPDVLARYDVTLIAD
                     PLRFPVLLAGGEEAGSGKREDGRHFARFVDPHPKPSYLFALVAGHLEKLERPYRTAEG
                     RDVMLRLWAEADAIGRCQYAMDALERAMRWDERAYGRNYDLPVFNVVATHDFNMGAME
                     NKGLNIFNSKYLLADRDTTTDDEYRHIEAVIGHEYFHNWSGNRVTCRDWFQLSLKEGF
                     TVFREHSFCEDLHSAALKRIEDVATLRRAQFPEDAGPLAHPVRPAEYQAIDNFYTATV
                     YEKGAELIRMLAGKLGREAFRRGTDLYFSRFDGKAATLEDLLAALGEANGVDLSPYLA
                     WYAQAGTPELRARGEYDATSRRYTLTLSQHTPATPGQPHKQPLPIPVNVALLADNGTA
                     FPLQLKDGTPAATERVLELTQAEQRFIFDDVASAPTPSLCRGFSAPVCVDFEYTPAQL
                     ASLVLHEAEGFDRWNAAQELAGRAFDELLANRSERQALVAWLDALAQSFATRGRSL"
BASE COUNT          340 a          660 c          585 g          297 t
ORIGIN      
        1 tggtaaaatg atccaatgga cacgcaagcc agcaatgagc cgcaagccac ggatggagtc
       61 gttcgcctcg ccgagtatcg gccgccggcc tggcgggtca cccatgtcga actcaccttc
      121 gagctcggca tcgacgagac cgtcgtccgc tcgaagctca ccctcgcgcg cgaccgcgac
      181 gagcccttgc gcctcgacgg cgaggacatt gcgctcctcg ctgcacgcct ggacggccgg
      241 cggctcgacg acagcgaata ctccctcgat gccgctggcc tcacggttcc cgccgcacgc
      301 gacggcagcg tcctcgaact ggacgtgcgc gtcgaacccg cgcgaaacag ccagttgtcg
      361 ggtctgtact tgtccggtgc gcctgaacgc ggcttcctgc tgacccagtg cgaggcacag
      421 ggttttcgtc gcatcacgtt cttccccgac cggcctgacg tgctggcacg ctatgacgtc
      481 accttgattg cggacccctt gcggtttccg gtactgctgg ccggcggcga ggaagccgga
      541 tcgggcaaac gggaagacgg ccgccacttc gcgcgattcg tcgacccgca cccgaagccg
      601 agctacctgt tcgcgctggt cgccgggcac ctcgaaaagc tcgagcggcc ctaccgcacc
      661 gcggaaggcc gcgacgtgat gttgcgccta tgggccgagg ccgacgcgat cggccgctgc
      721 cagtacgcga tggacgcact ggaacgcgcg atgcgctggg acgagcgcgc ctatggccgc
      781 aactacgacc tcccggtgtt caacgtggtc gcgacccacg atttcaacat gggcgcgatg
      841 gaaaacaagg ggctgaacat cttcaactcg aagtacttgc tggccgaccg cgacaccacc
      901 accgacgacg agtaccgcca catcgaggct gtaatcggcc acgaatactt ccacaactgg
      961 agcggcaacc gcgtcacctg ccgcgactgg ttccagcttt ccctgaagga aggcttcacc
     1021 gtgttccgcg aacattcgtt ctgcgaggac ctgcactcgg ccgccctgaa gcgcatcgag
     1081 gacgtcgcga ccctgcggcg tgcgcagttc cccgaggacg ccggcccgct cgcgcatccg
     1141 gtgcgtccgg cggaatacca agccatcgac aacttctaca cggcgacggt ctacgagaaa
     1201 ggcgcggaac tcatccgcat gctggcaggg aagctcgggc gcgaggcgtt ccggcgcgga
     1261 accgacctgt acttctcgcg cttcgacggc aaggcggcca ccctcgagga tctgctggcg
     1321 gcattgggcg aagccaacgg cgtggatctc tcgccgtatc tcgcgtggta cgcacaagcc
     1381 ggaacaccgg agcttcgcgc gcgcggcgaa tacgacgcaa cgtcaaggcg atacacgctc
     1441 acgctttcac agcacacccc ggcaacgccc gggcaaccgc acaagcagcc gctgccgatc
     1501 ccggtgaacg ttgcgctgct ggcggacaac ggcacggcgt ttccgctgca gctcaaggac
     1561 ggcacccccg ccgctaccga gcgcgtcctg gaactgacgc aagccgaaca gcggttcata
     1621 ttcgatgacg tcgcctcggc accaacgccc tcgctgtgcc gcggattttc ggccccggtg
     1681 tgcgtcgact tcgagtacac acccgcccaa ctcgcctcgc tcgtgcttca cgaagcggaa
     1741 ggcttcgatc gctggaatgc agcgcaggaa ctggccggcc gcgcgttcga cgagctgctc
     1801 gcgaatcgat ccgaacgaca ggcgctcgtc gcgtggctgg acgcgctggc gcagtcgttc
     1861 gcgactcgcg ggcgatcgct cg
//
LOCUS       DASUCJ010001284         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_74959, whole genome shotgun sequence.
ACCESSION   DASUCJ010001284 DASUCJ010000000
VERSION     DASUCJ010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74959"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFU90_15015"
     CDS             <1..797
                     /locus_tag="VFU90_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481153.1"
                     /translation="DILHTFFTPAVMADIRDNLHATYVRTGWIPDEFKFETVHWKRED
                     EGMDAICGAGLKAMIVVPNPQDDAKGVADLISSVHAFFDRYTKREFGCLRYAEITNEA
                     DLPRNGFDGVEQYAKYYESVAPIVASFGVTVITSGTSGKDLPWTARLAQILRDADPRP
                     PVDGYGFHPYGVPPDQMAEATLEMRRAAGTPAGGQPPNVYVTEIGQENAADLYRTIVD
                     LARATPAITIYEYLPQPGEDSRYGVKNDPARYRAVQRAWEYLHPHG"
     gene            complement(815..2185)
                     /locus_tag="VFU90_15020"
     CDS             complement(815..2185)
                     /locus_tag="VFU90_15020"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481154.1"
                     /translation="MHPRRALKAPLATVFLLCACSMPGGYERGALPGGYAPLESRDLS
                     FTPQKTPLRHIVVVIQENRTFDDFFATYPGADGTTTGKLHNGKVVKLRKIPLEGSPLS
                     NGWSSFTTAYDGGKMDGFDLDSWGGPGKAGLRPYTYVDPSQIKPYWDMARQYVLGDHM
                     FQGQGSGSFTAHQELIAGGEDVGPKRSLIDWPAPPHPGAYAWGCDAPKGTVTSLITRR
                     DEYLDRKGPFPCLTYESLAEQLDAHHLTWRYYSAPVVRGGAGEMWNAFDAIKSVRYGS
                     DWKNDISTPSKTIFSDLVKGKLANVTWVTPSVPDSDHEHAGPDRGPSWVAQVVDAIGR
                     SPDWDSTAIIVVWDDWGGHYDHVPPRQLGFGNLGFRVPLLIVSPYVHAGQIAHTQYEF
                     GSVLKFIEQNWGLHPMQAGDQRATSIGGIFDFRQKPRPFTPIQSALPRSFFENEPVSN
                     LPGDGE"
     gene            complement(2264..>3625)
                     /locus_tag="VFU90_15025"
     CDS             complement(2264..>3625)
                     /locus_tag="VFU90_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481155.1"
                     /translation="LTESVAYSNAPFFSDPYSITFSGVQLPNQPLQRAGVVFDYKAPH
                     SVLEWLADAQYTGQNNPNNLPAYTTFDAGVSAALTRGTLTFAASNVTNTFGGVFASPA
                     NAVPYATVGGFTIPTIARPLAPRSYSLTYGIKFGEGVTTSQTGAAFAAPRGSQFAGRA
                     GTPGGQGGGFRNLFSPLPTAPPDAPFAVATNNDRCAAADAAQAETLSGELQTFTTRIE
                     AAKTAAVYPATLPAPALADASVLYHGLGNTYALTIVPKGAGRLRALAGCFALHVARAD
                     DAAARHLYAPKTALFFVPQLNFMPAVGLYFIARQQQAGQESFRVYKLPAVAPKTPFEV
                     RAAGGECSGDARNLAVEALGELQRYFSSGAKAPNWTIASYAAAGGTWYDLDPSDPSVF
                     AALLQCGRIAATTADELKQRGFDGRPAPELNYAAPLGLYFVRPNNGFGPGGAGRPQPS
                     PSP"
BASE COUNT          671 a         1150 c         1155 g          649 t
ORIGIN      
        1 aagacatcct gcacaccttt ttcacgcccg ccgtcatggc cgacatccgc gacaacctcc
       61 acgccacgta cgtgcgcacc ggatggattc cggacgagtt caagttcgaa accgttcact
      121 ggaagcgcga agacgaagga atggacgcca tctgcggcgc cgggctcaag gcgatgatcg
      181 tcgtaccgaa tcctcaagac gacgccaagg gcgttgccga ccttatttcc agcgtccacg
      241 cgtttttcga tcgttacacc aaacgcgagt tcggctgcct gcgctacgcc gagattacga
      301 atgaagccga tcttccgcgc aacggtttcg acggcgtcga gcagtatgcg aagtactacg
      361 agtccgtcgc accgatcgtg gcctccttcg gcgttacggt aatcaccagc gggacgtcgg
      421 gcaaggactt gccctggacg gcgcgccttg cgcagattct gcgcgatgcc gatccgcggc
      481 cgccggtcga cggctacggc tttcatccgt acggggttcc gccggaccaa atggcggaag
      541 cgacgctgga aatgcgacgc gcggccggca cgcctgcggg cggccagccg cccaacgtgt
      601 acgtcaccga aatcggtcag gaaaatgcgg cggatctcta ccgcacgatc gtcgatctcg
      661 ctcgcgcgac gccggcgatt acgatatacg aatatctacc ccaacccggc gaagattcgc
      721 ggtacggcgt caagaacgac ccggcacgct atcgcgccgt gcagagagcg tgggagtacc
      781 tgcacccgca cgggtaacgc gccgcgccgc cgccctactc gccgtcgccc ggcaaattcg
      841 aaacgggttc gttttcgaaa aacgatcttg gtaaagccga ctggatcggc gtgaagggtc
      901 gcggcttctg ccgaaaatcg aaaatcccgc cgatgctggt ggcgcgctga tcgccggcct
      961 gcatcggatg caggccccag ttctgttcga tgaacttcaa gacgcttccg aactcatatt
     1021 gcgtatgtgc gatctgacca gcgtgcacgt acggcgaaac gatcagaagc ggcaccctga
     1081 acccgagatt gccgaatccg agctggcgcg gcggcacgtg atcgtaatgg ccgccccagt
     1141 cgtcccacac gacgatgatc gcggtcgagt cccagtccgg actccgaccg atcgcgtcga
     1201 ccacctgcgc gacccacgaa gggccgcgat ccggcccggc atgctcgtga tcggaatcgg
     1261 gaaccgacgg tgtgacccac gtaacgttcg cgagcttgcc tttgacgagg tcgctgaaga
     1321 tggtttttga gggcgttgaa atgtcgttct tccaatccga cccgtaccgc acgcttttga
     1381 tcgcgtcgaa tgcgttccac atttcgccgg cgccgccgcg cacgaccggt gccgagtaat
     1441 atctccaggt gaggtggtgg gcgtcgagtt gttcggccag cgactcgtag gtgaggcacg
     1501 ggaaaggccc cttgcgatcc aggtactcat cgcgacgggt gatgagcgac gttaccgtgc
     1561 ccttcggcgc atcgcaaccc catgcgtacg ctcccggatg cggcggcgcg ggccagtcga
     1621 tcagactgcg cttcggacca acgtcctcgc cgccggcgat cagctcctgg tgggccgtaa
     1681 agctcccgct gccttggcct tggaacatgt ggtcgcccag aacgtattga cgcgccatat
     1741 cccagtacgg ctttatctgc gacgggtcga cgtaggtgta cggtcgcagt cccgctttgc
     1801 ccggacctcc ccaggagtct aaatcgaagc cgtccatctt cccaccgtcg tatgcggtcg
     1861 taaacgacga ccacccgttc gaaagcgggg agccctccaa cggaatcttg cgcagcttga
     1921 cgaccttccc attgtgaagt tttccggtcg tggtgccgtc cgctcccgga taggttgcga
     1981 aaaagtcatc gaacgtccgg ttttcttgaa tcactaccac gatatgccgc agcggcgttt
     2041 tttgcggcgt aaatgaaaga tcgcgcgatt ccaatggggc gtagccgccc ggcaaagcgc
     2101 cacgctcgta gccaccgggc atcgaacacg cacacagcag aaagacggtt gctaggggcg
     2161 cctttaacgc gcgacgcggg tgcaccgccc gttcttagcg cagtgggaac gcggttcccg
     2221 tgcgtacggc acgcggccgg tgtcgccaag cgtctcggcg cctttagggc gacggacttg
     2281 gctggggacg tccggctccg cccggtccga agccgttatt cgggcgcacg aagtagagtc
     2341 cgaggggagc ggcgtagttc aactccggcg caggtctccc gtcgaagccg cgctgcttga
     2401 gttcatccgc ggtggttgcc gcgatccggc cgcactgcag gagcgcggcg aatacgctgg
     2461 ggtcggaagg atccagatcg taccacgttc cgcccgctgc ggcgtacgac gcgatcgtcc
     2521 agttgggggc tttcgcaccg ctggaaaagt atcgctgcag ctcgccgagc gcttcgactg
     2581 caagattgcg cgcgtctccc gagcattcgc ccccggcggc gcgcacttcg aagggcgttt
     2641 tcggcgcgac cgccggcaac ttgtaaaccc gaaacgactc ttgaccggcc tgttgctgac
     2701 gcgcgatgaa gtacaagcca acggccggca tgaagttcag ctgcggaacg aagaacagtg
     2761 ccgttttggg ggcgtacaaa tggcgcgcgg cggcgtcgtc cgcgcgcgca acgtgcaggg
     2821 cgaagcagcc cgcgagcgcc cgcaagcgtc ccgcgccctt gggtacgatc gtcagcgcgt
     2881 aggtattgcc cagtccgtga tacaggacgc tcgcatccgc aagagccggc gccggcaagg
     2941 ttgcggggta caccgccgcc gtcttcgccg cttcgatccg agtggtaaac gtttgcagct
     3001 cgccggagag cgtttcggcc tgcgccgcat cggcggcggc acagcgatcg ttgttggtcg
     3061 caacggcgaa gggagcatcc ggaggcgcgg ttggaagcgg cgaaaacaag tttcggaagc
     3121 ctccgccctg accgcctggc gtcccggcgc ggccggcaaa ctgcgatccc cgcggggccg
     3181 caaacgccgc cccggtttgc gaggttgtaa ccccttcgcc gaactttatg ccgtacgtca
     3241 gtgagtacga tctcggcgcg agggggcgcg cgatcgtcgg aatcgtaaaa ccgccgacag
     3301 ttgcgtacgg caccgcgttc gccggtgagg caaacacgcc gccgaaggtg ttcgttacgt
     3361 tgcttgccgc gaaggtgagc gtgccgcgcg tcagtgcggc gctgacgccg gcatcgaacg
     3421 tcgtatacgc gggaaggttg ttcggattgt tctgccccgt gtattgagcg tcggcgagcc
     3481 actcgagaac cgaatgcggc gccttgtagt cgaagacgac cccggctcgc tgcaacggct
     3541 ggttcggcag ctgaacgccc gagaacgtga tcgagtaggg atcgctgaag aacggcgcgt
     3601 tcgaataggc gaccgattcc gtgag
//
LOCUS       DASUCJ010001285         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1511694, whole genome shotgun sequence.
ACCESSION   DASUCJ010001285 DASUCJ010000000
VERSION     DASUCJ010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511694"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>1090)
                     /locus_tag="VFU90_15030"
     CDS             complement(1..>1090)
                     /locus_tag="VFU90_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5481156.1"
                     /translation="SYVLGGRGMEVVYNEGQLASYAESAPPILPDAPLLVDKYLRGLE
                     AEVDAAFDGEDILIPGIFEHIERAGIHSGDSISVYPTQTISEEMKQRIVDVTTAIARE
                     LNIRGLINIQFVIHNGQLYIIEANPRASRTVPIIQKATGVNLVAAATRIALGEKLRDM
                     PYGTGLAPRAPFVVVKIPVFSFAKMRGVETILGPEMKSTGEVLGIDQTFAGALRKGFI
                     AAGVRLPNEGGRILVSIADEEKEESIPILRRYVALGHTLIATPGTREHLQANGIPCEQ
                     INKIADGSPHVLDLIHERRVDLVINDAQGPRQISDNYRIRRAAVEANIACLTSLDTAR
                     ALAEALENTAGPPRCLQEYRCAQTQVRS"
BASE COUNT          185 a          347 c          343 g          215 t
ORIGIN      
        1 ttaggaacga acttgcgtct gagcgcatcg atactcttgc aggcagcgcg gcggacccgc
       61 ggtgttttcg agcgcctcag cgagcgcgcg cgccgtgtcc aggctggtca ggcaggcgat
      121 gttcgcctcg accgcggcgc ggcgaatgcg gtagttgtcg gagatctggc gcggcccttg
      181 cgcgtcgttg atcaccaaat cgacgcgccg ctcgtgaatg aggtcgagca cgtgcggcga
      241 tccgtccgcg atcttgttga tctgctcgca cgggatgccg ttcgcctgga ggtgctctcg
      301 cgtacccggc gtcgcgatga gcgtgtggcc cagagcgacg taccggcgca gaatcggaat
      361 cgattcttct ttttcttcat cggcgatcga gacgagaatg cgcccgcctt cgttcggcag
      421 ccgaacgccc gcggcgatga accctttgcg cagcgcgccg gcaaacgtct gatcgatgcc
      481 gagcacctca cccgtcgact tcatctccgg tccgagtatc gtctccacgc cgcgcatctt
      541 cgcgaacgag aagacgggaa tcttcacgac gacgaacgga gcgcgcgggg ccaagcccgt
      601 gccgtacggc atgtcgcgca acttctcgcc cagcgcgatg cgggttgccg ccgcgaccaa
      661 attcacgcct gtcgcctttt gaatgatcgg aactgtgcgg ctcgcacgcg gattcgcttc
      721 gatgatgtac agctgcccgt tgtgaatgac gaactggatg ttgatgagtc cgcggatatt
      781 gagttcgcgc gcgatcgccg tcgtgacgtc gacgatgcgc tgcttcatct cctccgagat
      841 cgtctgcgtc ggatagacgc tgatcgaatc gccggagtgg atgccggcgc gttcgatatg
      901 ctcgaagatg cccggtatca ggatgtcctc gccgtcgaac gccgcgtcga cttccgcttc
      961 caggccgcgc agatacttgt cgaccaggag cggcgcatcc ggaagaatcg gcggcgccga
     1021 ctccgcgtac gatgcgagct ggccttcgtt gtacacgact tccataccac gcccaccgag
     1081 cacgtacgac
//
LOCUS       DASUCJ010001286         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1221230, whole genome shotgun sequence.
ACCESSION   DASUCJ010001286 DASUCJ010000000
VERSION     DASUCJ010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221230"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFU90_15035"
     CDS             complement(<1..287)
                     /locus_tag="VFU90_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEU5481157.1"
                     /translation="MPDTLTATIAGAASEVDAAVWNELVAATDPHGNPFLDHAFFRAV
                     EDSGSATRRTGWTPRHLVLRDGDRVVGLLPMYEKAHSQGEYVFDHGWANAF"
     gene            complement(280..1035)
                     /locus_tag="VFU90_15040"
     CDS             complement(280..1035)
                     /locus_tag="VFU90_15040"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU5481158.1"
                     /translation="MPKLFDRPIAHRGLHDRTAGVIENSSAAFEAAIANGYAIECDVQ
                     LASDGVPFIFHDDDFDRLTAATGPSDAKPIAEVQKLVLTGSAAGDVPQTFDQFLAQVA
                     GRTLLQIELKMQRNAEKTRALAESVARSLSGYRGPYTLESFDPNLLVALRNAGVRAPL
                     GIITYGYDEPDWDTKVTAWQRLVLRNLLHWPMTRFDFISCRNVSLGLPAVRMFRALGV
                     PVTSWTIKSTADASAALEHADQIVFEGFLPAIA"
BASE COUNT          194 a          334 c          339 g          180 t
ORIGIN      
        1 tcgaacgcat tggcccagcc atgatcgaac acgtactcac cctgcgaatg tgccttctcg
       61 tacatgggca gcaggccgac gactctgtcg ccgtcgcgca acaccaagtg ccgcggcgtc
      121 cagcccgtcc gccgtgtggc cgaaccggag tcctccactg cgcggaagaa ggcgtggtcg
      181 aggaagggat tgccgtgcgg atcggtcgcg gcgaccagct cgttccagac cgccgcgtcg
      241 acctccgacg cggcgccggc gatcgtcgct gtaagagtgt caggcaattg cgggtaagaa
      301 gccttcgaac acgatctggt cggcgtgctc taatgctgcg ctggcgtccg ctgtcgactt
      361 gatcgtccag ctcgtcaccg gcacgccgag tgcgcggaac atccgcaccg ccggaaggcc
      421 gagcgacacg ttgcgacagg aaatgaagtc gaaacgagtc atcggccagt gcagcagatt
      481 acgcagcacc aggcgctgcc acgccgtcac cttggtgtcc cagtcgggct cgtcgtagcc
      541 ataggtgatg atgccgaggg gggcacgtac gcccgcgttg cgcaacgcga ccagaagatt
      601 gggatcgaat gactcgagcg tataaggacc gcgatagcca gacagcgacc gcgcgactga
      661 ctccgccaag gcccgggtct tttcggcgtt gcgctgcatc ttgagctcga tctggagcag
      721 cgttcgtccg gcgacctgcg caaggaactg atcgaacgtc tgcggtacgt caccggcagc
      781 gctgccggtt agaacgagct tctggacctc ggcgatgggc ttggcatccg aagggcccgt
      841 cgccgccgtt agccggtcga agtcgtcgtc gtggaagatg aacggcacgc cgtcgctcgc
      901 gagttgcaca tcgcattcga tcgcgtagcc gttcgcgata gcggcctcga acgccgccga
      961 cgaattctcg atcacccccg cggtgcgatc atgcaggccc cgatgcgcga tcgggcggtc
     1021 gaacagtttt ggcatcagac ttcgacg
//
LOCUS       DASUCJ010001287         1483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_670763, whole genome shotgun sequence.
ACCESSION   DASUCJ010001287 DASUCJ010000000
VERSION     DASUCJ010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670763"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFU90_15045"
     CDS             complement(<1..305)
                     /locus_tag="VFU90_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5481159.1"
                     /translation="MTFLYVQVLREPFERIPGIAPARRPRREPIVLSAREVGRVVEHL
                     DQPFRLCVLLMYGGGLRLLECLTLRVKDVDMERREIVVRAGKGDKDRRVPLAVSAIG"
     gene            665..>1483
                     /locus_tag="VFU90_15050"
     CDS             665..>1483
                     /locus_tag="VFU90_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5481160.1"
                     /translation="MLCLDHWIAGRRDHGSSDRAGTIYNPATGEKTGEVPLATAADID
                     RAVAAARAAFPAWSRTGLGKRATVLFAFRELVRQHADELARAIVNEHGKVLADARGEV
                     QRGLEVVDFACGIPQLIKGEFSENVSTGVDSYSLRQPLGVVAGITPFNFPVMVPMWMF
                     PIAIAAGNSFILKPSEKDPSPSLMLAELWREAGLPNGVFNVVHGDRVAVERLLEHPDV
                     AAVSFVGSTPIARHVYETATRHGKRVQALGGAKNHMVVLPDADLELAADAAVNAG"
BASE COUNT          262 a          522 c          472 g          227 t
ORIGIN      
        1 ataccaatgg cgctcacagc cagcggcacg cgtcgatcct tgtcgccttt gcccgcccga
       61 acaacgatct cgcgtctttc catatcgaca tccttcacgc ggagcgtcag acactcgagc
      121 agacgcaagc cgcctccata catcagcagc acacagagtc gaaatggctg gtcgaggtgc
      181 tccacgacgc ggcccacctc gcgtgccgac aagacgatcg gctcgcgacg tggccgccgg
      241 gcgggcgcga tcccgggaat gcgctcaaag ggttcgcgca acacctgcac atacagaaac
      301 gtcaatgccg atagcgcctg gttgtggctc gatgccgaca gtcggccaat gaattcccgc
      361 acttccgtcg ggccgagctc acgcgggtga cggcgatcgt agtgcaggat gtaccgccga
      421 atccaataga cgtacgttgc ggtcgtccgg tcggagtaac gacgctcgcg agcacgctga
      481 cgcacgatct cgagcaggcg aaagcggcgt tcggggtcgg gcgcaccgac gaggtgatgc
      541 tgcggaatgc ggggtcgctc tggcacgagt gcaaccttcc ccggagcacg gaacccgcag
      601 gatcaatcca ccgccaatcg taccacagcg ttgacaacgc ccacgctcgg cgggatagtc
      661 cgacatgctt tgcctcgacc actggatcgc cggccgtcgc gaccacggct cctccgaccg
      721 cgccggcacg atctacaatc cggcaaccgg cgagaagacc ggcgaagtcc ccctcgccac
      781 ggccgccgac atcgaccgcg ccgtcgccgc ggcgcgcgcc gcgttccccg cctggagccg
      841 cacgggactc ggcaaacgcg ccaccgttct cttcgccttc cgtgagctcg ttcgccagca
      901 cgccgacgaa ttggcgcgcg ccatcgtcaa cgagcacgga aaggtcctgg ccgacgcgcg
      961 gggcgaggtg cagcgcggcc tcgaggtcgt cgactttgcg tgcggcatcc cccagctcat
     1021 caagggcgag ttctccgaga acgtctccac cggcgtcgac agctactcgc tgcggcagcc
     1081 gttaggcgtc gtcgccggga tcacgccgtt caacttcccg gtgatggtgc cgatgtggat
     1141 gttcccgatc gccatcgcgg ccgggaacag cttcattctc aagccgtcgg agaaggatcc
     1201 gtctccctcg ctgatgctcg ccgagctgtg gcgcgaggcc gggctgccga acggggtgtt
     1261 caacgtcgtc cacggcgacc gggttgccgt cgagcggctg ctcgagcatc ccgacgtcgc
     1321 cgcggtcagc ttcgttggct cgacgccaat cgcgcgccac gtgtacgaga cggcgacccg
     1381 gcacgggaaa cgcgtgcagg cgttaggcgg cgccaagaac cacatggtcg tgctccccga
     1441 cgccgacctc gagctcgccg cggacgcggc ggtgaacgcc ggg
//
LOCUS       DASUCJ010001288         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1343445, whole genome shotgun sequence.
ACCESSION   DASUCJ010001288 DASUCJ010000000
VERSION     DASUCJ010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343445"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..683
                     /locus_tag="VFU90_15055"
     CDS             237..683
                     /locus_tag="VFU90_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5481161.1"
                     /translation="MIERRGSGATSFLVGLAVGAGVALLFAPQTGEETRRQLRRRARR
                     AAHLARDAAGDLSDNVVDRYEQAKRSVEDRLDSAREALEIRKHQAQEAFRAGREAAQQ
                     ARLDLEARIAQSKAHYRAATSQTRVARRSTLAPTEDAKNSADPESE"
     gene            734..>1644
                     /locus_tag="VFU90_15060"
     CDS             734..>1644
                     /locus_tag="VFU90_15060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5481162.1"
                     /translation="MPNQPVSLPRRFWAATKDYAKRIWDNAGEDNIFFLAGGIAFNIL
                     LAAVPFVLLLFSGLGYLLNQSEAQSAANLWRFIDQLLPPHSQNPDSPIYKIINDVIRA
                     RRSVGIYSLIGFVWFSTRLFGTLRSVLAEVFDIEHERSIIAGKVFDIEITVVSTILFV
                     AYTAVNAYLRLATTHGVAVLQRIGLQKEVMGRLEYAVGSVIASAFMVLLFFSLYKFLP
                     NRKIRWQYALLAAIVTSALFELAKALFTSYVGRFNPGSLYTGALTALVVIVMWVYYAA
                     VIFILGGEVGRVYELRRVRRLQRETLE"
BASE COUNT          303 a          521 c          539 g          281 t
ORIGIN      
        1 cacatgctgg gcgccacacc gggagaggtt cgcgccgaac tcgccgcgct ggtggaaccg
       61 gcggaagacg acgtcgagcc cgatgcggcg gcgcgaaacg tgaagagctt cggatccgtc
      121 aaggacaagg acgtggtccg acagaaatcg aagggtcgcc ggtgaggaga cgaggagatg
      181 aggacatgaa gcacaggacg cgctacgaag acgaagacga ggacgagccg tacgtcgtga
      241 tcgaacgccg cggcagcggc gcgacgtcgt ttctcgtcgg gctcgcggtc ggggcaggcg
      301 ttgcgttgct gttcgcgccg caaaccggcg aagagactcg ccgccaactg cgccgccgcg
      361 cgcgccgcgc cgcccacctg gcgcgcgacg cggcaggcga tctgtcggac aacgtcgttg
      421 accgatacga gcaagccaag cgcagcgtcg aagaccggct cgattcggcc cgcgaggcgc
      481 tcgagattcg caagcaccag gcacaggaag ccttccgtgc tggacgcgaa gcggcgcagc
      541 aggcgcggct ggacctcgag gcgcgcatcg cgcaatcgaa ggcgcactac cgcgccgcca
      601 ccagccagac acgggtcgcg cggcgatcca cgctcgcgcc taccgaagac gcgaagaact
      661 cggccgaccc ggagtccgag taggagctcc catctcctct tccgtcgttg ggccgacgac
      721 cgcggccaac gccatgccca accagccggt gtctctgccc cgacgttttt gggcggcaac
      781 gaaggattac gccaagcgaa tctgggacaa cgcgggcgaa gacaacatct tcttcctcgc
      841 cggcggcatt gcgttcaaca tcctgctggc cgccgtcccg ttcgtgctgc tcctgttctc
      901 gggactcggc tatctcctca accagtccga ggcacagtcg gccgcgaatc tgtggcgctt
      961 catcgatcag ctcctgccgc cgcacagcca gaaccccgac tcgccgatct acaaaatcat
     1021 caatgatgtg atccgcgcac ggcgctcggt cggcatctac agcctgatcg gattcgtgtg
     1081 gttctcgacg cgactgttcg gcacgctgcg cagtgtgctc gccgaagtgt tcgacatcga
     1141 gcacgagcgc agcatcattg cggggaaggt gttcgacatc gagatcaccg tggtgagtac
     1201 gattctcttc gtggcataca cggcggtgaa cgcctatctg cggctcgcga cgacgcacgg
     1261 cgtcgccgtg ctgcagcgga tcggcttgca gaaggaggta atgggccggc tcgagtacgc
     1321 ggtcggctcg gtaatcgcat cggccttcat ggtgctgctg ttcttttcac tgtacaagtt
     1381 tctgcccaac cgaaaaatcc gatggcaata cgcgctgctc gctgccatcg tgacgagcgc
     1441 cttgttcgag ctcgccaagg cgttgttcac gtcgtacgtt gggcggttca atccgggcag
     1501 cttgtatacg ggcgcgctga cggccctcgt cgtgatcgtg atgtgggtct attatgcagc
     1561 ggtgattttc atcctgggcg gagaagtcgg ccgggtgtac gagctgcgcc gcgtgcggcg
     1621 tctccagcgc gagacgctcg agga
//
LOCUS       DASUCJ010001289         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_930716, whole genome shotgun sequence.
ACCESSION   DASUCJ010001289 DASUCJ010000000
VERSION     DASUCJ010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930716"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFU90_15065"
     CDS             <1..528
                     /locus_tag="VFU90_15065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5481163.1"
                     /translation="RPGIVHRLDRDTSGLLVVAKTPDALTALGKAMKARRIRREYLGL
                     VRGVPEQPRGTIEGNIGRDPANRLKFAIVAEGKSAVTHYEVRAAFAKHAELVFRLETG
                     RTHQVRVHLAAMGHPLVNDRVYGRAEPRFDLPGQALHAWRLSFDHPRTGERLQFETPP
                     PEAYLQARELLRGDG"
     gene            521..>1121
                     /locus_tag="VFU90_15070"
     CDS             521..>1121
                     /locus_tag="VFU90_15070"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5481164.1"
                     /translation="MDKRPEERREFAFELRTADGNARRGTLTLPHGVVETPCFMPVGT
                     AATVKGLTPLELREAGAQIVLANAYHLWLRPGRTILEAAGGLHRFMAWDGPILMDSGG
                     FQVFSLESRRTLDDGGVTFRSHLDGSEHRFTPESVIDFAEAIGVDVAMVLDECPPWPC
                     ERDACAKAVARSYRWAQQCKQIATETGFLAGGHHVFAIAQ"
BASE COUNT          194 a          379 c          374 g          174 t
ORIGIN      
        1 cggcccggga tcgtccaccg gctcgaccgc gacacctccg ggctgctcgt cgtcgcaaaa
       61 acgcccgacg cgctgacggc gctcgggaaa gcgatgaaag cgcgccgtat ccggcgcgag
      121 tacctcggcc tcgtgcgcgg cgtgcccgaa cagccgcgcg gaacgatcga aggcaacatc
      181 ggacgcgacc cggccaaccg gttgaagttc gcgatcgtcg ccgaaggcaa atcggcggtc
      241 acgcactacg aagtgcgcgc cgcgttcgcc aaacatgccg agctggtctt tcgtctcgaa
      301 acggggcgca cccatcaggt ccgcgtgcat ctggcggcga tgggacatcc gctggtcaac
      361 gaccgcgtgt acggacgcgc cgaaccgcgc ttcgatcttc ccggccaagc ccttcacgcg
      421 tggcgccttt cgttcgatca tccgcgcacc ggcgagcggc tgcaattcga aacgccgcct
      481 ccggaagcgt atctgcaggc gcgcgagctg ttgcgcggcg atggataagc ggccggaaga
      541 gcggcgcgaa ttcgcgttcg aacttcgtac ggccgacggc aacgcgcgcc gcggcacgct
      601 gacgcttccg cacggcgtcg tagaaacgcc gtgctttatg ccggtcggca ccgcggcgac
      661 ggtgaaaggc ctgacgccgc tcgaactgcg ggaagccggc gcgcagatcg tgctcgccaa
      721 cgcctaccat ctgtggctgc gtcccggccg cacgatcctc gaagccgccg gcggactgca
      781 tcgcttcatg gcgtgggacg gaccgatctt gatggattcc ggcggttttc aagtcttcag
      841 tttggagagc cggcgcacgc tcgacgacgg cggcgtcacc tttcgctcgc acctcgacgg
      901 cagcgaacac cggttcacgc ccgagagcgt gatcgatttc gccgaagcga tcggggtcga
      961 cgtcgcgatg gtgctcgacg agtgcccgcc gtggccatgc gaacgcgacg cctgcgccaa
     1021 ggcggtggcg cgcagttatc gctgggcgca gcaatgcaag cagatcgcga ccgaaaccgg
     1081 gtttctcgca ggcggacatc acgtgtttgc gatcgcgcaa g
//
LOCUS       DASUCJ010001290         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1404449, whole genome shotgun sequence.
ACCESSION   DASUCJ010001290 DASUCJ010000000
VERSION     DASUCJ010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404449"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFU90_15075"
     CDS             complement(<1..189)
                     /locus_tag="VFU90_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEU5481165.1"
                     /translation="MAPRVGVVMGSRSDWETMRHAADMLASLGVAHEVEVVSAHRTPD
                     KLFAYAEDAAARGIEVIIA"
     gene            240..506
                     /locus_tag="VFU90_15080"
     CDS             240..506
                     /locus_tag="VFU90_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481166.1"
                     /translation="MDKRLLDILCDPVTKTPVRPLRRDELDALNHAISAGEVDTVAGR
                     KVATPLAAGLITTDRKVVYRIEDDIPVMLADEGIGTLQLASFPA"
     gene            718..>1010
                     /locus_tag="VFU90_15085"
     CDS             718..>1010
                     /locus_tag="VFU90_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEU5481167.1"
                     /translation="MVDIASRASDSSQDHVGGLLAAVRDMTLKESANLVAGLLDNVDD
                     TLFDLAEKAESNASQTEYFDGMRETRKRRPRIERLFADRVSHGFGEFAAGRSTR"
BASE COUNT          173 a          361 c          307 g          169 t
ORIGIN      
        1 ggcgatgatc acttcgatgc cgcgggcggc ggcgtcctcg gcgtaggcga acaacttgtc
       61 cggggtacgg tgcgccgaga ccacctcgac ttcatgggcg accccgagcg acgcgagcat
      121 gtccgccgca tggcgcatgg tctcccaatc cgaacgcgaa cccatcacca ccccgacgcg
      181 gggcgccact gtcgttttcc gcatggtgtg tccgtcgcaa aacgggtatt gtacgcgcca
      241 tggacaagcg attgctcgac atcctctgcg atccggtcac caagaccccg gtgcgcccgc
      301 tgcgccgcga cgagctcgac gcgctgaacc atgccatctc tgccggcgaa gtcgacaccg
      361 tggccggccg caaggtcgcc acgccgctcg ccgccggtct catcaccacc gaccgcaagg
      421 tggtctaccg gatcgaggac gacatcccgg tgatgctcgc cgacgaaggc atcggcacgc
      481 tgcagttggc gagctttccc gcctagcgtt tctcccgttc gggcaccccc gcattcccgc
      541 catggtgcgc tttgtgacac tttgtgaccg cgttgtgagc ggattcacgt tcccgcgact
      601 gcggaaaggg atacttagat tctccattct gccgccccaa gcggtaaacc ggttttcgac
      661 atgacgccga atccgtccga tctgccctcc gacatccagc gcaacgcggc caccaaggtg
      721 gtcgacatcg cctcgcgcgc gtccgactcc agccaggacc acgtcggcgg gctgctcgcg
      781 gcggtgcgcg acatgacgct caaggagagc gcgaacctcg ttgccgggct gctcgacaac
      841 gtcgacgaca cgctgttcga cctcgcggaa aaggccgaga gcaacgccag ccagactgaa
      901 tacttcgatg gcatgcgcga gacccgcaag cgccgtccgc ggatcgagcg gctgttcgcc
      961 gatcgcgtct cgcacggctt cggcgagttc gccgccggcc gttccacgcg
//
LOCUS       DASUCJ010001291         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_105611, whole genome shotgun sequence.
ACCESSION   DASUCJ010001291 DASUCJ010000000
VERSION     DASUCJ010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105611"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFU90_15090"
     CDS             complement(<1..1135)
                     /locus_tag="VFU90_15090"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU5481168.1"
                     /translation="MNTTTNRALAAALAGAIAIAGALMPAQPAAAQYAVAGAPGVANV
                     GIVTGDVVIVRGDSGEQVAATINTPLLPGDYISTGGGSRAEVQFDGISMLRLAQDTQV
                     RFVDLRAGSREMQLATGTAELAELQGIDGGAQIDTPSVTVRPNRAGDYRVSVMPNGDT
                     LVTVRSGSATVSSGAGTQTVTAGSTLVAYGAYSNPRITLEGAIAYGAFDRFNDERNAT
                     IVASYNSDPYLAPQLAGYTNFTGYGQWTNVPGYGQAWSPYDQSNDWMPYSNGQWVWEP
                     GYGYTWVGNEPWGYAPYHYGRWFNYNNQWMWQPPAYQYQTNGADLVASWLPALVGFFL
                     SGGSGNIGYPGYNGSIGWVPLAPGEAYNPWYPGFGGNQGYSQYG"
     gene            1325..1609
                     /locus_tag="VFU90_15095"
     CDS             1325..1609
                     /locus_tag="VFU90_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEU5481169.1"
                     /translation="MNESQKRFTRGEFVQSAVALPALAALLASTAAPAQAKGSQAQFK
                     YQSKPNGSKKCSNCTFFIPGKSATANGTCKIVDGTISPNGYCIAYSPKSS"
     gene            complement(1683..2090)
                     /locus_tag="VFU90_15100"
     CDS             complement(1683..2090)
                     /locus_tag="VFU90_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481170.1"
                     /translation="MSTIATQTYVRSSAEHAAERIYSAFGIEREGDAVKRLRAGPVAR
                     DAIVTLGKPERRPSLIRVAISWHVPDSGAFPHFRGFFEIRSCDGLACIALLGYYQPPF
                     GVAGRAFDAIAGHRIANATIAQLLAEVADAVSH"
     gene            complement(2156..>2532)
                     /locus_tag="VFU90_15105"
     CDS             complement(2156..>2532)
                     /locus_tag="VFU90_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5481171.1"
                     /translation="TTGWQLSLSLKAAAKMDNPEIFIVTIDRPELDIFFKDDFANRLE
                     RTYGRGNVVVDMSSVRFIDSTCLGKLAAMRRERARRDLPQEKLVISSPQMRRIFEIVR
                     FDTVWPIYDTLEEALETELQLL"
BASE COUNT          455 a          823 c          803 g          451 t
ORIGIN      
        1 cgccgtactg cgaatacccc tgattcccgc cgaatcccgg ataccacgga ttgtacgcct
       61 cgcccggtgc gagcgggacc cagccgatcg agccgttgta gcccggatag ccgatgtttc
      121 cgctgccgcc gctcaggaag aatccgacca gcgcgggcag ccacgacgcg accaggtcgg
      181 cgccgttggt ttggtactga taagccggcg gctgccacat ccactggttg ttgtagttga
      241 accagcgtcc gtagtggtag ggcgcgtagc cccacggctc gttgccgacc caggtgtagc
      301 cgtagccggg ctcccagacc cactgaccgt tggagtacgg catccagtca ttggactggt
      361 cgtacggcga ccatgcctga ccgtagccgg ggacgttggt ccactgcccg tagccggtaa
      421 agttcgtata gcctgcgagt tgcggggcca aatacggatc ggagttatac gatgcgacga
      481 tcgttgcatt gcgttcgtcg ttgaaacggt cgaacgcgcc gtatgcgatc gcgccttcca
      541 gcgtgatccg cgggttcgag tacgctccgt acgcgaccag cgtgctgccg gccgtcaccg
      601 tttgcgttcc ggcaccgctc gaaacggttg ccgatccgct gcgcacggtc accagcgtgt
      661 cgccgttggg catgaccgag acgcgatagt cgccggcccg gttggggcga accgtcaccg
      721 acggcgtgtc gatttgcgcg ccgccgtcta tgccttggag ctcggcgagc tcggccgtgc
      781 cggtcgccag ctgcatctcg cgcgagccgg cgcgcaggtc gacgaagcgc acctgcgtgt
      841 cttgcgccag ccgcagcatc gaaatgccgt cgaactggac ctcggctcgg gagccgccgc
      901 cggtcgaaat gtagtcgccg ggcagcagcg gggtgttgat ggtagcggcc acctgctcgc
      961 cgctatcgcc gcgaacgatt accacgtcgc ccgtgacgat gccgacgttc gcgacgccgg
     1021 gtgcccccgc gaccgcatac tgcgctgccg cgggctgcgc cggcatcagc gcccccgcga
     1081 tcgcgatcgc tccggccagc gccgccgcca gagctcgatt cgttgtagtg ttcatgattg
     1141 cgttttctcc tcgcattgct atcaacggag acttaccccg cagcgtacgc gctttcggca
     1201 ccggaggggg ttaatattca ctgaaaacag gcgaccgagg ggttccacga cgcgcgtttt
     1261 acgcccgatc gttagaggcg gattatagcc ggcatgcgta tcttccattc cggaggttac
     1321 cgccatgaac gagtcccaga agaggttcac gcgcggcgag ttcgtgcaga gcgccgtcgc
     1381 gctgcccgcc ctcgccgcct tgctcgcaag caccgccgcg ccggcgcaag cgaaaggttc
     1441 ccaagcgcaa ttcaagtatc aaagcaaacc caacggcagc aagaaatgct cgaactgcac
     1501 gttcttcata ccgggcaaaa gcgcgaccgc caacggcacg tgcaagatcg tcgacggcac
     1561 gatcagcccg aatggttact gcatcgcgta ctcacccaaa tccagctgac gccgtcgcgt
     1621 cgccgaaaag caagagcgct ccgcatcgcc ggagcgcttt tgccttaatc gagcgctgcg
     1681 gcttagtgtg aaacggcgtc cgccacttcc gcgaggagct gcgctatcgt ggcgttagcg
     1741 atgcggtgtc ccgcgatggc gtcgaacgcg cggcccgcta cgccgaacgg cggctggtag
     1801 tagccgagca gcgcgatgca cgcgagccca tcgcacgagc gaatttcgaa aaaccctcga
     1861 aaatgcggga acgcgccgct gtcggggacg tgccacgaaa tcgcgacgcg aatcagggag
     1921 ggccggcgtt cgggctttcc gagcgtaacg atcgcgtcgc gcgccaccgg tccggcccgc
     1981 agccgcttga ccgcatcgcc ctcgcgctcg atgccgaagg cgctatagat ccgctcggcg
     2041 gcgtgctcgg cgctgctgcg aacgtaggtc tgcgtggcga tcgtcgacac gcgcgaattc
     2101 tcgcaccttt ttgagacaat tcggtgaagg ttcgaggtag aaccggtagg gcgcgctaca
     2161 ggagctgcag ctcggtttcc agcgcctctt ccagcgtgtc gtagatcggc cagaccgtgt
     2221 cgaagcggac gatctcgaag atgcggcgca tctgcggcga cgaaatcacg agcttctcct
     2281 gcggcaagtc gcggcgcgcc cgttcgcggc gcatggcggc gagtttgccg agacacgtcg
     2341 agtcgataaa ccgcaccgac gacatatcca ccacgacgtt tccgcggccg tacgtccgct
     2401 ccaaacgatt cgcgaaatcg tctttgaaaa agatgtcgag ctcgggccgg tcgatcgtga
     2461 cgatgaaaat ctctgggttg tccatcttgg cggccgcctt gagcgaaaga gaaagctgcc
     2521 acccggtcgt gt
//
LOCUS       DASUCJ010001292         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_13824, whole genome shotgun sequence.
ACCESSION   DASUCJ010001292 DASUCJ010000000
VERSION     DASUCJ010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13824"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFU90_15110"
     CDS             <1..412
                     /locus_tag="VFU90_15110"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU5481172.1"
                     /translation="TCFGAPFMVHHPTTYSRLLGKKIDAHDVECWLVNTGWTGGAYGV
                     GRRMSIGHTRAMVNAAIEGRIAADFQTERFFGLRIPRHVPGVPDEVLDPRAAWADPAA
                     YDAAAKKLAGLFAENFKRFEAHASDAVKAIAIKP"
     gene            499..948
                     /locus_tag="VFU90_15115"
     CDS             499..948
                     /locus_tag="VFU90_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481173.1"
                     /translation="MPTFAGFDHVDCRVPSLAAVESFYDALMPELGLPRKRFSYVDEA
                     GEWREASAEHTYNTVEYFEEPQSDRATFFIGFIERPDHVPGFTRIAFRVDLGRMMELE
                     TLLKRIGARHVERSADLDAYPAIFFEDPGGTKLEIVARAARALELAG"
     gene            1020..>1247
                     /locus_tag="VFU90_15120"
     CDS             1020..>1247
                     /locus_tag="VFU90_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481174.1"
                     /translation="MKIAFLAALLAALSLPISASAQVAQPAPAQMQRGSGPAVYHHWM
                     KRLSTLNLSAQQQQQVQNVLGQYASQHPVGSA"
BASE COUNT          219 a          417 c          384 g          227 t
ORIGIN      
        1 gacctgtttc ggtgcgccgt tcatggtcca tcatcccacc acgtattcgc ggctgctcgg
       61 aaagaagatc gacgctcacg acgtcgagtg ctggctggtg aacacggggt ggaccggcgg
      121 cgcgtacgga gtcggccgtc gtatgtcgat cggacatacc cgtgcgatgg ttaacgccgc
      181 catcgaaggg cgcatcgctg ccgactttca aaccgaacgg tttttcggat tgcgtatccc
      241 gcgccacgtt cccggggttc ccgacgaggt gctcgacccg cgcgccgcct gggcagatcc
      301 ggcggcctac gatgccgcag ccaagaaact cgccggcctc ttcgccgaga acttcaagcg
      361 attcgaagcc cacgcgtccg atgccgtgaa ggcgatcgcg atcaaaccgt aaggaacccc
      421 gcaagaagtt gatgcaccga tcgttgaggc cgcaccgggt gcgagcgcga gcgacctcct
      481 gcggacttac ctagcaacgt gccgacgttc gccggtttcg accacgtcga ttgccgcgtg
      541 ccgtcccttg ccgcggtcga atccttctat gacgccctga tgcccgagct gggattgccg
      601 cgcaagcgtt tctcgtacgt cgacgaggcc ggtgaatggc gcgaagcatc ggcggagcat
      661 acgtacaaca ccgtcgaata cttcgaggag ccgcagtcag atcgcgcgac cttcttcatc
      721 ggtttcatcg agcgcccgga ccacgtcccg gggttcaccc ggatcgcgtt ccgcgtcgac
      781 ctcggccgca tgatggaact cgaaacgttg ctgaagcgga tcggtgcgcg gcacgtcgag
      841 cgttccgccg acctcgacgc ctacccggcg attttcttcg aagatcccgg cgggacgaag
      901 ctcgagatcg tcgcgcgggc cgctcgcgcg ctggagctcg ccgggtagcc cttcgggcga
      961 gcgggcatcc taatgcgccc ttcaggcaca cctcagcgag ctcgggctat gataggcgca
     1021 tgaaaatcgc cttcctggcc gcgcttcttg cggccctttc tctgccgatt tcggcttcgg
     1081 cccaagtcgc ccagccggca ccggcgcaaa tgcagcgcgg atcgggtccg gccgtttatc
     1141 accactggat gaagcgtctc tcgacgctca atctatccgc gcaacagcag cagcaagtgc
     1201 agaacgtgct cggacagtac gcctcgcagc atccggtcgg cagcgcg
//
LOCUS       DASUCJ010001293         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1343507, whole genome shotgun sequence.
ACCESSION   DASUCJ010001293 DASUCJ010000000
VERSION     DASUCJ010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343507"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFU90_15125"
     CDS             <1..294
                     /locus_tag="VFU90_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481175.1"
                     /translation="AQSGRFPAVDVLASASRTMAAVAAGDHVRDARAVRNALALLERI
                     DDARRLGIEPGDPASQRAIAAEAQLDDFVLQDREDSAPLATLAALHRIAEYLA"
     gene            329..>1136
                     /locus_tag="VFU90_15130"
     CDS             329..>1136
                     /locus_tag="VFU90_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481176.1"
                     /translation="MKPMRLRDPCRPAAILCLFALLGAAAPATAPVTLASILEAHLNA
                     RKALKIAPVRTVQTEGTVAGLGLQGTFHTWRDGGNERYDETLGIRTQRTLRVNGREFV
                     QNANGDVRELHGLAQRRQVTEDFIDSGDFARHPENDVLIGAARLADGREIWQLRVTPP
                     GGEPYGVGLDKQTAMVDEKAYLNGDSLTTVDYSDYRVAGGVLYPVVQVESSGDHAYDI
                     TSRVTALQANRQIDPSVFAAFVPAVVDAPSPVTLALLSGDGHFFVRASGNG"
BASE COUNT          197 a          385 c          378 g          176 t
ORIGIN      
        1 gcgcaaagcg gccgttttcc cgcggtcgac gtgctcgcca gtgccagccg cacgatggcg
       61 gcggtcgccg ccggcgacca cgttcgggac gcgcgcgccg tccggaacgc gctggcgctg
      121 ctcgagcgga tcgacgacgc ccgccgtctg ggaatcgaac ccggcgatcc cgcctcgcag
      181 cgggcgatcg cggccgaagc acagctcgac gatttcgtgc tgcaagaccg cgaggattcc
      241 gcgccgctcg ctacccttgc cgcgctgcac cggatcgcgg aatatctggc ctgacgcaca
      301 atgcaccacg ggccgccggc gcgaagagat gaaaccgatg cgcttgcgcg atccgtgccg
      361 tccggcggca attttgtgcc ttttcgctct gctcggtgcc gccgcaccgg cgacggcgcc
      421 ggtcaccctg gcgtcgattt tggaagcgca cttgaacgcg cgtaaggcgc tcaagattgc
      481 gccggttcgc accgtgcaaa ccgaagggac ggtcgccgga ctcggtttgc aaggcacgtt
      541 tcacacctgg cgcgacggcg ggaacgagcg atacgacgaa acgctcggca tccgcacgca
      601 gcgaacgctg cgcgtgaacg gacgcgagtt cgtgcaaaac gccaacggcg acgtccgcga
      661 actccacggg ctggcgcagc gccggcaagt gaccgaggat ttcatcgatt cgggcgactt
      721 cgcgcggcat ccggaaaacg acgtcttgat cggtgcggcg cgcctcgcgg acggccgcga
      781 gatctggcag ctgcgcgtaa cgccgcccgg cggcgagcca tacggagtcg gactcgacaa
      841 gcaaacggcg atggtcgacg agaaagccta cctcaacggc gactcgctga cgaccgtcga
      901 ctactccgac tatcgcgtcg ccggcggagt actctatccg gtggttcaag tggaatccag
      961 cggggatcac gcctacgaca ttacctcgcg cgtcaccgca ctgcaggcga accggcagat
     1021 cgatccgtcg gtattcgcag ccttcgtgcc ggcggtggtc gacgctccgt cgccggtaac
     1081 gctggcgctg ctgtcgggtg acggtcattt cttcgttcgc gcaagcggca acggac
//
LOCUS       DASUCJ010001294         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_854056, whole genome shotgun sequence.
ACCESSION   DASUCJ010001294 DASUCJ010000000
VERSION     DASUCJ010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854056"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFU90_15135"
     CDS             <1..1002
                     /locus_tag="VFU90_15135"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU5481177.1"
                     /translation="PGIEVPEVVRELSTSQILTMTRAGGSRPEDVGRMTPRRRVAAAG
                     RIARFVLEPALVHGVFHADPHEGNVLVRRNGTIAVLDFGMVGRLSDDLRRGLADMFAA
                     MQRGDARRLTDRLVQLAPPARPVDRSLLVQRLQRLLERYFGDSAERPHIGKALGEIME
                     LVRTYGLRSPGSLALLFKAVALADGTVLAITPDKPLTQYLEPIAKRVRLARMSAGDWG
                     ERARLSAMDVKELSLELPHHADRVLTDLELGNLRVWTRIEDAEPMLGRLELMVEHANA
                     TMIAAACVVGVTVLFAVYRPPWWQTAAAWAFWIALLIALVVTVRTGLRVFFHRRVRR"
     gene            complement(1026..>1218)
                     /locus_tag="VFU90_15140"
     CDS             complement(1026..>1218)
                     /locus_tag="VFU90_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481178.1"
                     /translation="VANTKGGNKLVELTPTGKILATKTIDTSKTGHIFGLLASGTSDS
                     DTVLYYTDTKTNTLQELMQ"
BASE COUNT          179 a          395 c          403 g          241 t
ORIGIN      
        1 ccgggtatcg aggtgccgga agtcgtgcgc gagctatcga catcgcagat cctcacgatg
       61 acgcgcgccg gcggcagccg tccggaggat gtcggccgta tgacgccgcg ccgtcgcgtg
      121 gccgcggccg gacggatcgc gcgctttgtg ctggagccgg ccttggtcca cggcgttttc
      181 cacgccgatc ctcacgaggg caacgtcctc gtccggcgca acggtacgat cgccgttctc
      241 gacttcggca tggtcggacg cctatccgac gatctgcgcc gcgggctggc cgacatgttc
      301 gcggcaatgc aacgtggcga cgcacggcgc ctgaccgatc gcctggtgca actcgcgccg
      361 ccggcccggc cggtcgatcg cagcctgctc gtacagcgcc tgcagcgtct attggaacgc
      421 tacttcggcg attcggctga acggccgcac atcggcaagg cgttaggcga gatcatggag
      481 ttggtgcgca cgtacgggct gcgctcgccc gggtccctgg cgctcttgtt caaggcggtc
      541 gcgctagcgg acggcacggt gttggcgatc acacccgaca aaccgctaac gcagtatctc
      601 gagccgatcg ccaagcgcgt gcgtctcgcg cgcatgtcgg ccggcgattg gggcgaacgc
      661 gcgcgtcttt cagccatgga cgtaaaggag ctgagcttgg agctcccgca ccacgccgac
      721 cgcgtgctca ccgatttaga gctcggcaat ctgcgcgtct ggacgcgaat cgaagacgcc
      781 gaaccgatgc tcggacggct ggagctcatg gtcgagcacg cgaacgccac gatgatcgcg
      841 gccgcctgcg tggtgggcgt caccgtgctc ttcgcggtgt accgcccgcc gtggtggcaa
      901 accgccgccg cttgggcgtt ttggattgca ctgctgatcg cgctggtcgt gacggttcgc
      961 accggcctgc gcgtgttctt ccatcgaagg gtgcgccgtt agcggcgttc ccgtatgcgg
     1021 ctcttctact gcatgagttc ttgcagcgtg ttcgtcttgg tatcggtata gtacagcacc
     1081 gtatcgctat cgctcgtacc gctcgccagg aggccgaaga tgtgaccggt cttactcgtg
     1141 tcgatcgtct tggtggccag aatctttccg gtcggcgtca gctccacgag cttgtttcca
     1201 cccttggtat tcgcgacg
//
LOCUS       DASUCJ010001295         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1175319, whole genome shotgun sequence.
ACCESSION   DASUCJ010001295 DASUCJ010000000
VERSION     DASUCJ010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175319"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFU90_15145"
     CDS             complement(<1..191)
                     /locus_tag="VFU90_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481179.1"
                     /translation="MGRFLPPDHSKGDERTLGGYTAVHARPAAFEGCDGRSYSVSIET
                     DATGDAAAPFGAYLLFVQW"
     gene            complement(216..1151)
                     /locus_tag="VFU90_15150"
     CDS             complement(216..1151)
                     /locus_tag="VFU90_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5481180.1"
                     /translation="MLRRTFLYLSNQPRLFHFVRHNGMAKKFASRFVAGETLDTALAA
                     VRQLNARKIRASLDLLGESVTNEAEARAASRSYLQMLDRINEAGLDANVSVKLTQMGL
                     DISEELCVDIMQQILERARSYRTFVRLDMEGSDYTDRTLRLFEDRLYPSFPEHVGIVL
                     QSYLYRTQVDVERAIQLKCRVRLCKGAYNEPATVAFPEKRDVDANYVKCMQQLLLNGT
                     YPGIATHDPAILAQAKRFVRAQSIPPERFEFQMLYGVRRDLQDQIVREGFNMRVYVPF
                     GTQWYPYLMRRLAERPANVAFITGNMVRELVAGRH"
     gene            complement(1251..>2976)
                     /locus_tag="VFU90_15155"
     CDS             complement(1251..>2976)
                     /locus_tag="VFU90_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5481181.1"
                     /translation="LGIFPGAGGTQRLPRLIGLRAALDLILTGRNVRARKALQLGLVD
                     EMVHPSILREIAMDRARQLADGTLARSRGRASRGVAAVVLEDNPLGRRLIFSRARAGV
                     LAKTMGNYPAPIAAIDAVRAGYELGRDRGYAEEARLFGEMAVSEVSRQLVYLFFATTA
                     LKKDSGTAVPAAPLPVRKLGVLGAGFMGAGIASVAAMQGVIVRLKDAEYGRVAKGLAA
                     VSAVIGERLQKKQITRRQFEDQMTLVGGTTDYSGFSSCDLVIEAVFEDIDLKHRVLQE
                     AEQVMPSDAIYASNTSTIPISRIAEVSKRPEQVLGMHFFSPVHKMPLLEVIVTPRTTA
                     VATSTAVAFGKKLGKHVIVVNDGPGFYTTRTLAAFLNEAGYLLDEGVSIEALDRALID
                     FGFPVGPITLMDEVGLDVGAKVATVLRDAFGDRLTPSQSLSRVVASGRLGRKGGSGFY
                     RYDAPGGKKTGVDDTIYTLLPTGANRTEIPVLEMQQRCVLAMVNEAARCLEEGILRSA
                     RDGDVGAVFGIGFPPFRGGPFRYVDSVGLAHIVRQLEVYHGRFAPRFEPARILHDRAA
                     QGGGRWLD"
BASE COUNT          533 a         1052 c          899 g          492 t
ORIGIN      
        1 ctccattgca cgaacaggag atacgcgccg aagggcgccg ccgcatcgcc ggtcgcatcc
       61 gtctcgatcg acacactgta cgagcgacca tcgcatcctt cgaacgccgc cggtcgcgcg
      121 tgcaccgccg tgtagccgcc gagcgtccgc tcgtcgcctt tcgagtgatc gggtggaagg
      181 aacctaccca cgacacatct gcgccggcca acgcatcagt gccggcccgc gacgagttcg
      241 cgaaccatgt tgcccgtgat gaacgcgacg ttcgccggac gctccgcgag ccggcgcatc
      301 aaatacggat accactgtgt gccgaacggc acatacaccc gcatgttgaa cccttcgcgc
      361 acgatctgat cctgcaggtc acgcctaacg ccgtacagca tctggaactc gaagcgctcc
      421 ggcgggatgc tctgcgcgcg cacgaatcgc ttggcctgcg ccaggatcgc cggatcgtgc
      481 gtggcaatgc ccggataggt gccgttcagc aggagctgct gcatgcactt cacgtaattc
      541 gcatccacgt cgcgcttctc cggaaacgcg accgtcgccg gctcgttgta cgcgcccttg
      601 cacaaccgca cgcgacactt gagctggatc gcccgctcca cgtccacctg cgtgcggtag
      661 agatagctct gcaacacaat gccgacgtgc tccggaaagc tcggatacaa ccggtcctcg
      721 aacaatcgga gcgtgcgatc cgtgtagtcg ctgccctcca tgtccaaccg cacgaacgta
      781 cggtagctgc gtgcacgctc gaggatctgc tgcatgatgt cgacacacag ctcttcgctg
      841 atgtcgagcc ccatttgcgt gagcttcacc gagacgttcg catcgagtcc tgcctcattg
      901 atccggtcga gcatctgcag gtacgaccgg ctcgccgcgc gcgcctccgc ttcgttggtc
      961 acgctctcgc ctaacaaatc gagcgacgcg cgaatcttcc gggcgttgag ctggcgcacg
     1021 gccgccagcg ccgtgtccag cgtttcgccg gcaacgaacc gtgacgcgaa cttcttcgcc
     1081 atgccgttgt gacgcacgaa gtggaacagc cgaggttggt tcgacagata gaggaaagtt
     1141 cgacgcagca tgtcgtctga tgatcgggac ggagaatgcg actcggccga tgaacgcctt
     1201 acagacacac ggcggcgcat aagctagccc tcgcgttcac ggccatccag tcaatcgagc
     1261 cagcgtccgc cgccttgcgc cgcgcggtcg tgcagaatgc gagccggctc gaagcgcggc
     1321 gcgaaccggc catgatacac ctccagctga cgcacgatgt gcgcgagccc gaccgaatcc
     1381 acgtatcgga agggcccgcc gcggaacggc gggaagccga tgccgaacac ggcgccgaca
     1441 tcgccatccc gcgccgagcg aagaattccc tcctcgaggc accgcgccgc ttcgttcacc
     1501 atggcgagca cgcatcgctg ttgcatctcg agcacaggaa tctccgttcg attcgctccc
     1561 gtgggcagca gcgtgtagat ggtgtcatcc acgccggtct tcttgccgcc cggtgcgtcg
     1621 tagcgataga acccgcttcc gccttttctg cccaaccggc ccgacgcgac gacgcgcgac
     1681 aacgactgcg atggcgtgag ccggtcgccg aacgcgtcgc gcaacacggt tgcgaccttg
     1741 gcgccgacgt cgaggccgac ctcgtccatg agcgtgatcg gcccaacggg aaagccgaag
     1801 tcgatcagcg cgcgatccag cgcttcgatc gacacaccct cgtccaacag gtagcccgct
     1861 tcgttgagaa acgccgccag cgtgcgcgtg gtgtagaagc ccggcccgtc gttcaccacg
     1921 atcacgtgct tgccgagctt cttgccgaac gcgacggccg tcgacgttgc cacggccgtc
     1981 gtacggggcg tcacgatcac ctcgagcagc ggcatcttgt gcaccggtga gaaaaagtgc
     2041 atgccgagca cttgctcggg ccgcttcgat acttcggcga ttcgcgaaat cgggatggtg
     2101 ctcgtgttgg acgcgtagat cgcgtcggac ggcatcacct gctcggcttc ctgcaatacg
     2161 cggtgcttga gatcgatgtc ctcgaacacc gcttcgatca ccaggtcgca gctcgagaat
     2221 ccggagtaat cggtcgtccc gccgacgagc gtcatctgat cctcgaactg acgccgcgtg
     2281 atctgctttt tctgcaggcg ctcgccgatc acggcgctca ccgccgcgag gcctttggcg
     2341 acgcggccgt attccgcgtc cttgagccgg acgatcacgc cctgcatggc ggccaccgac
     2401 gcgatgcccg cgcccatgaa cccggcgccc aagacgccta acttgcgcac gggcagcggc
     2461 gccgcgggca ccgcggtccc cgagtccttc ttgagcgccg tggtcgcgaa gaacagatac
     2521 accagctggc gcgacacctc ggacaccgcc atctcgccga acagacgcgc ttcctcggcg
     2581 tacccgcggt cgcggcccag ctcgtagccg gcgcgcacgg cgtcgatggc ggcgatcggc
     2641 gccggatagt tgcccatcgt cttggcgagc acgccggcgc gcgcccgcga aaagatgaga
     2701 cgtctgccta acgggttgtc ctcgagcacc acagccgcca ccccgcgcga cgcccggccg
     2761 cgcgaccgcg ccagcgtgcc atcggccagc tgccgcgccc ggtccatcgc gatctcgcgc
     2821 aggatggacg gatgcaccat ctcgtccacc aagcctaact gtagcgcctt gcgcgcccgc
     2881 acgttgcgcc cggtgagaat caggtccaac gccgcgcgca ggccgatcaa acgcggcagg
     2941 cgctgcgttc cgcccgcacc cgggaagatg ccgagc
//
LOCUS       DASUCJ010001296         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1083605, whole genome shotgun sequence.
ACCESSION   DASUCJ010001296 DASUCJ010000000
VERSION     DASUCJ010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083605"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="VFU90_15160"
     CDS             complement(<1..1572)
                     /locus_tag="VFU90_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481182.1"
                     /translation="MLVGEGAAAFVSVPLASGKILIGQGAADPAAQSVSGDGTLSAAG
                     VLTISGLQGVTLPAPATGYLLFGQGSGLSWNGPLSQPTATAFYIDPAGGSDAAAGTSL
                     APLKTFREAVGRIGSFRPAFPSSATVTFHILSSQTDLSDPIVWSPVSGNTVFVGTPIL
                     QATSALANVSYSAGGVVTATIAGASLGDMVVNNAKGGVAFVGNVVGSTCTLSVPSASN
                     VPFSTAAPAAVLNWANGDSYSRYTLPQVYAPNVGCDITSAYTANIQLQHLNVGITGHS
                     YMATGTVGMLECAVTTPYTSSTVFFDTRGGSFINCFVSGQLQFGGGYFYAGALYNATG
                     DASAIVIERVAIAGGALLCGNGGMYLELSEVSSLNVASTNPNINLIGNNNFGSGSPST
                     IYGTYTLNLASGLTGESVGCLTYFGTAAATFLGNPTFTFGAGSQAGAVSFGNALDVGT
                     GVAQWYPHRALYVTNLDLAVALGGFGGRAFSDFGSFITNGTQGTTAPGTLATTDWYIN
                     AATGSDANAGTSSGAP"
BASE COUNT          268 a          510 c          554 g          246 t
ORIGIN      
        1 cggcgcgccc gagctcgtgc ccgcgttggc gtcgctgccc gtcgcggcgt tgatgtacca
       61 gtcggtggtc gcgagcgtgc cgggcgccgt cgtgccctgc gtgccgttcg tgatgaacga
      121 tccgaagtcc gagaacgcgc ggccgccgaa gccgccgagg gccacggcga gatcgaggtt
      181 cgtgacgtag agggcgcggt gggggtacca ctgggcaacg cccgtgccga cgtcgagcgc
      241 gttgccgaaa gagacggcgc ccgcttggct gcccgcgccg aacgtgaacg tggggttacc
      301 gaggaacgtg gccgcggcgg tgccgaagta cgtcaggcag ccgaccgatt cgcccgtaag
      361 cccgctcgcg aggttgagcg tatacgtgcc gtaaatcgta ctcggggatc cgctcccaaa
      421 gttgttattg ccgatcagat tgatgttggg gttcgtcgat gcgacgttga gcgaagagac
      481 ctcgctgagc tcgaggtaca tgccgccatt gccgcaaagc agcgccccgc cggcgatcgc
      541 gacgcgctcg atcacaattg cggaggcgtc gcctgtcgcg ttgtagaggg cgcccgcata
      601 gaagtagccg ccgccaaact gcaattgccc agatacgaag caattgatga atgagccgcc
      661 gcgcgtgtcg aaaaagacgg tgctcgacgt gtacggcgtc gtcaccgcgc actcgagcat
      721 cccaacggtg ccggtggcca tgtacgaatg gcccgtgatg ccgacgttga ggtgctgcag
      781 ttggatgttc gccgtgtacg cgctcgtgat gtcgcagccg acgttcggcg cgtaaacttg
      841 aggcagcgtg tagcgagaat acgagtcgcc gttggcccag ttgaggacgg ccgcaggggc
      901 cgccgtcgaa aacggcacgt tgctcgcgct cggcactgag agcgtgcacg tgctgcccac
      961 gacgttgccg acgaaggcaa cgccgccctt tgcgttgttc acgaccatgt cgccgagcga
     1021 tgcgcccgcg atcgtggccg tgacgacgcc gccggccgag tacgagacgt tcgcgagtgc
     1081 gctcgtcgcc tgcaggatcg gcgtgccgac aaataccgtg ttgccgctga cgggcgacca
     1141 gacgatagga tccgaaaggt cggtttgcga actgaggata tggaacgtga cagtcgcgct
     1201 cgaggggaac gcaggccgga agctgccaat gcgcccgacg gcctcgcgaa acgtcttgag
     1261 cggcgcgagg ctcgtgcccg ccgcggcgtc gctgccgccc gccggatcga tgtagaaggc
     1321 cgtggccgtg ggctgcgaca gcggcccgtt ccacgagagc cccgagcctt gcccgaagag
     1381 caggtaaccc gtcgccggcg ccggcagcgt gacgccctgc aggcccgaga tcgtgagcac
     1441 gcccgccgcc gacagcgtgc cgtcgccgct cacgctctgc gcggccggat cggctgcccc
     1501 ttgcccgatc aggatcttgc ccgacgcgag agggaccgag acgaacgccg ctgccccctc
     1561 gccgacgagc accccgtg
//
LOCUS       DASUCJ010001297         1488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_518234, whole genome shotgun sequence.
ACCESSION   DASUCJ010001297 DASUCJ010000000
VERSION     DASUCJ010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518234"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFU90_15165"
     CDS             complement(<1..329)
                     /locus_tag="VFU90_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481183.1"
                     /translation="MSGVRVLVGTRKGAFVLTSDERRREWKVDGPHFAGWEIYHVKAS
                     PLNPDRIYASQSSGWFGQLIQRSDDGGRTWQAAGNDFAYAGIPGTHQWYDGTPHPWEF
                     ARVWHLEP"
     gene            447..869
                     /locus_tag="VFU90_15170"
     CDS             447..869
                     /locus_tag="VFU90_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481184.1"
                     /translation="MLRTAILAAIAFALPAPSLAAGTCAGADPAIVSVVVANVTPAGG
                     VDQYHLTGTVTNRGTAGQASNVLQFVDIYQQDVRLDAKSIPPLGPGQSANFDYVALRS
                     TDAGKGTTTLNFRLRFRQPVPPGAADCDAANDTFSLNF"
     gene            complement(858..1307)
                     /locus_tag="VFU90_15175"
     CDS             complement(858..1307)
                     /locus_tag="VFU90_15175"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEU5481185.1"
                     /translation="MDLVATVPRSPKDKLAGLVSLKRTIDKAQAHNEGRLGEYDYDCP
                     HDKPLFEFLGTNGDEFAEKVRELKSDEAIVDWLRSSGLGRKSQAQIDAFNADRMRWHP
                     EPGSHSAEYFADLREKIAPGRPDIVTWFDLLDLDEGRPVPEATRLEV"
BASE COUNT          250 a          495 c          461 g          282 t
ORIGIN      
        1 ggctccaggt gccaaacgcg cgcgaattcc cacggatgcg gcgtgccgtc gtaccactga
       61 tgcgttcccg gaataccggc gtaggcaaag tcgttcccgg ccgcctgcca ggtgcggccg
      121 ccgtcgtcgg accgctgaat caactgtccg aaccagccgc ttgactgcga ggcgtagatc
      181 cggtccggat tgagcggcga cgctttgacg tgatagattt cccacccggc gaagtgcgga
      241 ccgtcaactt tccactcgcg acgccgctcg tcggaggtga gaacgaacgc gcctttgcgc
      301 gtacctacca aaactcgtac tccgctcatc caggcctcct ttgcgcccga gtcgttacaa
      361 cgcctcccgg cctgtattcg ttaggctgcg gcggcggcag ggtaagaaaa aaagctcgtc
      421 ccgctcggga cggagggagg gtttgtatgc ttaggactgc gatcctcgcg gcgatcgcgt
      481 tcgcgttgcc ggcgccgagc ctcgcggccg ggacctgtgc gggcgcggac cccgccatcg
      541 tttcggtcgt cgtggcgaac gtcacgccgg cgggcggcgt cgaccagtat cacctcaccg
      601 gtacggtaac caatcgcggt accgcaggac aggcttccaa cgtcctgcag ttcgtcgaca
      661 tctaccagca agacgttcgg ctcgacgcca agagcattcc gccgctcgga ccgggccaat
      721 cggccaactt cgactacgta gcgctgcgct cgaccgatgc cggcaaaggc acgaccacgc
      781 tcaatttccg gctgcgcttc cggcagccgg tgcctccggg tgcggcggat tgcgacgccg
      841 ccaacgatac gttctcgcta aacttctaag cgggtcgctt ccggcaccgg ccgtccctcg
      901 tcgaggtcga gcagatcgaa ccacgttacg atatccggcc ggcccggcgc gatcttctcg
      961 cgcagatctg cgaagtattc cgccgaatgc gagcccggct ccggatgcca gcgcatccgg
     1021 tcggcattga aggcgtcgat ttgcgcctgc gacttgcggc cgagtccgga cgaccgcaac
     1081 cagtcgacga tcgcctcgtc gctcttcagt tcgcggacct tctcggcaaa ttcatcgccg
     1141 ttggtgccga gaaactcgaa cagcggcttg tcgtgcggac agtcgtaatc gtactcgccc
     1201 agccggcctt cgttgtgtgc ctgagccttg tcgatcgttc gcttgagcga aacgaggccc
     1261 gccagcttgt ccttcgggct gcgtggaacg gtggccacga gatccatggg gtttcctcct
     1321 gatgattttg cagtcgttcg agcgcatatc gcgccggctc gggtaagaac ccattcatga
     1381 aggaatccgt ttcgccggac ggcatcggcg tcgacgcgat cggtccggcc acgacgattc
     1441 tcgacgtgcg ccggcgtgcc ggaaaacgtc agattcgcgg cgcgctgc
//
LOCUS       DASUCJ010001298         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_29038, whole genome shotgun sequence.
ACCESSION   DASUCJ010001298 DASUCJ010000000
VERSION     DASUCJ010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29038"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFU90_15180"
     CDS             <1..889
                     /locus_tag="VFU90_15180"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEU5481186.1"
                     /translation="VSIAVPIVANWIRAYMIVMLGYLSDNRIATGIDHIIYGWLFFGL
                     VMLIMFWIGSYWREDEVVPDPGAPVPAQADREGRSQSHRWIVAATALVAIVVWPIAEG
                     AIERNATAAPVTLAMIDAPGWQASAAPAGSFTPHFQNPSAVRHEILSKGDRTLELYVG
                     YYQGQSGNRKLISSENVLLRAEDHAWHATRDGTVSMNIGGRAVPVRATTIRSADGRAL
                     ETWLFYWIDGKLTSSDAIAKALVTWLRVTGRYDGSAVVVACAPSGEGVDASSTLQEFL
                     GTAWPAIAKVLDDARGSAR"
     gene            910..>1472
                     /locus_tag="VFU90_15185"
     CDS             910..>1472
                     /locus_tag="VFU90_15185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5481187.1"
                     /translation="MDAGHDARPLIAHVVFRFDIGGLENGVVNLINGMPSERYRHAAV
                     SLTDITSFRDRVLRRDVEFVALGKRPGHGIRLFPRLYGTFRRMRPAIVHTRNLAALEA
                     SIPAAVAGVPVRVHGEHGRDVGDLDGSSRRYRLVRRIHRPFVTHYVALSQDLEGYLVN
                     YIGVPSRKVSRIVNGVDTERFAPVSSRA"
BASE COUNT          238 a          458 c          504 g          272 t
ORIGIN      
        1 cgtatcgatc gcggtgccga tcgtcgcgaa ctggattcgc gcgtacatga tcgtgatgct
       61 cgggtatctt tccgataatc ggatcgcgac cggcatcgat cacatcatct acggctggct
      121 gttcttcggc ctcgtgatgc tgatcatgtt ctggatcggt tcgtattggc gcgaggacga
      181 ggtcgtgccc gatccaggcg caccagttcc tgcgcaggcg gaccgcgagg ggcgatcgca
      241 gtcgcaccgg tggatcgtcg cggcgacggc actggtcgcc atcgttgtgt ggccgatcgc
      301 ggaaggtgcg atcgagcgca atgcgaccgc tgcgcccgtc acgctcgcca tgatcgatgc
      361 gcccggatgg caggcgagcg ccgcacccgc ggggtccttc acgccgcatt tccagaaccc
      421 gtcggcggtg cgccacgaga tcctttccaa aggcgatcgt acgctcgagc tctatgtcgg
      481 ctattaccag ggacaaagcg gaaatcgcaa gctgatctcg tcggaaaacg tactgctgcg
      541 cgccgaggat cacgcatggc acgcgacgcg cgacggcacg gtgtcgatga atatcggggg
      601 acgcgcggtc ccggtacgcg cgacgacgat ccgcagtgcc gacggcagag cgctcgagac
      661 gtggctcttc tactggatcg acgggaagct cacgtcgagc gacgcgatcg cgaaggcgct
      721 cgtgacctgg ctgcgcgtca ccgggcgcta cgacggctcg gcggtggtgg tcgcatgcgc
      781 cccgtcgggc gaaggcgtcg acgcgtcgtc gaccctgcag gaattcctcg gtacggcatg
      841 gccggcgatc gcgaaagtgc tggacgatgc gcgagggtcg gcaaggtgag gagtccgccg
      901 acgcgaccgg tggacgcggg gcacgacgcg cgtccgctga ttgcgcacgt cgtattccgc
      961 ttcgacatcg gcggcctcga gaacggtgtc gtcaatctca ttaatgggat gccttccgag
     1021 cgctatcggc acgccgcggt ctcgctgacc gacatcacga gctttcgtga ccgtgttctg
     1081 cgtcgcgacg tcgagttcgt cgcgctaggc aagcggcccg gccatgggat caggctgttc
     1141 ccgcgcctct acgggacgtt tcgccgtatg cggccggcaa tcgtgcatac gcgcaacctc
     1201 gcagcgctcg aagcgagcat tcccgcggcg gtcgccggtg ttccggtacg cgtgcacggc
     1261 gagcacggac gcgacgtcgg cgatctcgac ggctcgagtc gccggtaccg gctcgtgcgg
     1321 cggattcatc gacccttcgt cacgcactac gttgcgcttt cgcaggatct ggaagggtat
     1381 ctggtgaact acattggagt gccgtcgagg aaggtgtcgc ggatcgtcaa cggcgtggat
     1441 accgagcgct ttgcaccggt gtcctctcgt gc
//
LOCUS       DASUCJ010001299         1393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_808645, whole genome shotgun sequence.
ACCESSION   DASUCJ010001299 DASUCJ010000000
VERSION     DASUCJ010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808645"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFU90_15190"
     CDS             complement(<1..199)
                     /locus_tag="VFU90_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481188.1"
                     /translation="MSTRRAARNFALAAGIFLASLLIPAAWAAEAEDASRTRAAADSA
                     GAAAPAPPFAMLIARVFVNTVN"
     gene            complement(174..944)
                     /locus_tag="VFU90_15195"
     CDS             complement(174..944)
                     /locus_tag="VFU90_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEU5481189.1"
                     /translation="MIRRPRLLSRLAVPVLALLTAWCTALQAGEFTINPLRVSLDRAT
                     RSGEITVRNDDTVPLRMQLEAMTWRQDGDGRDQYEPTDELIFFPRAMEIPPGESRVVR
                     VGVRAAPVTREDAYRLFIEELPPPADHQSASQGTSLRIFLRVGVAVFVAPAQPKRTGE
                     IARLDLRAGTVEWSVVNTGNAHFRAERVEVAGIGRDGSRLFVQDVPERYFLAGVAKTL
                     RFDIPRDTCKQLGALEASVIGENLELKRKLDVDPARCP"
     gene            complement(941..>1393)
                     /locus_tag="VFU90_15200"
     CDS             complement(941..>1393)
                     /locus_tag="VFU90_15200"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HEU5481190.1"
                     /translation="RCDRLRQRQHHGVAVKRFVPWAVALLLLGVCSLATAQSCTFNGS
                     GGSIGFGSLDPSVATTRTAFIDLKVKCTPASGTTPTFQFSGSNGNAPLRMKHASQNAF
                     IPYSVAASFQGTTGANQNWRVTATVLGTDYQNALVGSYSDILTATVVP"
BASE COUNT          230 a          463 c          486 g          214 t
ORIGIN      
        1 gattgaccgt gttcacgaag acgcgcgcga tcagcatcgc gaacggcggc gccggagccg
       61 ccgcgccggc gctgtccgca gcagcgcggg tgcgggacgc gtcctcggcc tcggccgccc
      121 acgcggccgg gatcagcagc gatgcgagga agatgccggc ggcaagcgcg aagttacggg
      181 cagcgcgccg ggtcgacatc gagctttcgc ttcagttcga ggttctcgcc gatcaccgaa
      241 gcctcgagcg cgccgagctg cttgcacgtg tcgcggggaa tgtcgaaccg cagcgtcttg
      301 gcgacgccgg cgagaaagta gcgctccgga acatcctgga cgaatagccg gctaccgtcg
      361 cggccgatcc cggccacttc gactcgctcg gcgcggaaat gcgcgttgcc cgtattgacg
      421 accgaccatt cgaccgtccc cgcacgcaaa tcgagacgcg cgatctcgcc ggttcgcttc
      481 ggctgcgccg gtgccacgaa caccgcgacc ccgacgcgca ggaagatccg gagcgacgtt
      541 ccctgggatg cgctctggtg gtccgcgggg ggcggcagct cctcgatgaa cagccggtag
      601 gcatcttccc gggtcaccgg cgcggcgcgc acacctacgc gcacgacgcg cgactcgccc
      661 ggtggaatct ccatcgcccg cggaaagaag atgagctcgt ccgtgggctc gtattggtcc
      721 ctgccgtcgc catcctggcg ccaggtcatc gcctcgagct gcatgcgcag cggcacggta
      781 tcgtcgttgc gcaccgtgat ctcgcccgac cgggtcgcgc gatcgaggct tacccgcagt
      841 ggattgatcg tgaattcgcc ggcctggagc gccgtgcacc atgcggtcag cagcgcgagc
      901 acaggcacag ccaggcgcga gagcaggcgg ggacgccgga tcatgggacc accgtggcgg
      961 tgaggatgtc cgagtacgaa ccgacgagcg cattctggta atcggttccg aggacggtgg
     1021 cggtgacgcg ccagttctga ttcgcccccg tggtgccctg gaacgaagcc gcgacgctgt
     1081 acggtatgaa cgcgttctgg gacgcatgct tcatccgcag cggtgcgttg ccgttgctgc
     1141 cggagaactg gaaggtcggc gtggtgcccg acgccggcgt gcacttcacc ttcaggtcga
     1201 tgaacgcggt ccgggttgtc gccaccgacg ggtcgagcga gccgaatccg atcgagccgc
     1261 cgctgccgtt gaacgtgcag ctctgtgccg tggcgagaga gcacacgccc agcagcagca
     1321 gtgcgacggc ccatggcacg aagcgcttca cggcgacacc gtgatgctga cgttgccgga
     1381 gtcgatcgca gcg
//
LOCUS       DASUCJ010001300         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_319150, whole genome shotgun sequence.
ACCESSION   DASUCJ010001300 DASUCJ010000000
VERSION     DASUCJ010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319150"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1086)
                     /locus_tag="VFU90_15205"
     CDS             complement(139..1086)
                     /locus_tag="VFU90_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU5481191.1"
                     /translation="MPINRPAALTLDARSLSPATKTEQILDAFDKLQVGTALEINEES
                     DPRALRNEMTQLRTGRFSWDARNLGSNRWTIRLERIDENADGETLLAHVPQFTQAKAS
                     TIKELAGQMSERSYKSGDTIFDEGEMWPYLGIVKTGKVIYTLLSPDGKTHTIGERLTH
                     DTLNESGTFDGGGATTRAEALTDATVVTLPSEAVIHAARNDAELALGFLIASSQARRR
                     SIDTIADLAFAHVLQRVAKFLLGYARASVGMTRGLPGVENLSQAQIAAAAGTVRDMAA
                     RALLRLKNADAVELDRGRVKAIDRARLEAFAHNVQAPPV"
     gene            1140..>1430
                     /locus_tag="VFU90_15210"
     CDS             1140..>1430
                     /locus_tag="VFU90_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481192.1"
                     /translation="MSTSIHDDDVVILAGARTPFGNLGGVLRDLTATDLAVCAAEAAI
                     ARSGVPKERIDEVVFGNVIQTSADAIYLARHVGLRAGLPIAVPALTLNRLCGS"
BASE COUNT          199 a          460 c          502 g          269 t
ORIGIN      
        1 gacacatgcg ggacgctccg ctcagccctt cgactgcgcg cctgcggcgc tccgctcagg
       61 atgacgcatg cgggacgctc cgctcagccc ttcgactgcg cgcctgcggc gctccgctca
      121 ggatgacacg cagtggagtt agacgggtgg ggcttggacg ttgtgcgcga acgcttccag
      181 gcgcgcgcgg tcgatcgctt tgacgcggcc gcggtcgagc tcgacggcgt cggcgttttt
      241 caagcgcagc agcgcgcgcg ccgccatgtc gcggaccgtg ccggccgccg cggcgatttg
      301 cgcctgggat agattttcga cccccggaag tccgcgggtc atgccgaccg atgcgcgcgc
      361 gtagccgagc aagaacttgg cgacgcgctg cagcacgtgg gcgaatgcga gatcggcgat
      421 cgtgtcgatc gagcgacgcc gggcctgcga cgacgcgatc agaaagccga gcgccagctc
      481 ggcgtcgttg cgtgcggcgt ggatgaccgc ttcgctgggc agggttacga cggtcgcgtc
      541 ggtgagcgct tcggcgcgcg tcgtcgctcc gccgccgtcg aacgttccgc tttcgttgag
      601 cgtatcgtgg gtgaggcgct cgccgatcgt gtgggtcttt ccgtcgggcg aaagaagcgt
      661 gtagatcacc tttccggtct tgacgatgcc gagatacggc cacatttcgc cttcgtcgaa
      721 gatcgtgtcg cccgacttgt agctgcgctc gctcatctgc ccggccagct ctttgatggt
      781 gctcgctttg gcttgcgtaa actggggaac gtgcgcgagc agggtttcgc cgtcggcgtt
      841 ttcgtcgata cgttcgagcc ggatcgtcca gcgattgctt ccgagattgc gcgcgtccca
      901 ggaaaaccgg cccgtcctca gctgcgtcat ctcgtttcgg agcgcgcgcg ggtcgctctc
      961 ctcgttgatt tcgagcgccg ttccgacttg cagtttgtcg aacgcgtcga ggatttgctc
     1021 ggtcttggtg gccggcgata gagagcgagc gtcgagcgta agggcagcgg gacggttgat
     1081 cggcataggc ggccggtctt agggatggcc cgccgccgtg cctgccgaag ggccgcgcca
     1141 tgagcacctc gatccacgac gacgacgtcg ttatcctcgc cggagcccgc acgccgttcg
     1201 gcaacctcgg cggcgtgctg cgcgacctta cggccaccga tctcgcggtc tgcgcggccg
     1261 aagcggcgat cgcacgcagc ggcgtgccga aagaacgcat cgacgaagtc gtgttcggca
     1321 acgtgatcca gaccagcgcc gacgcgatct acttggcgcg gcacgtgggg ctgcgggccg
     1381 ggctgccgat cgcggttccg gcgctgaccc tcaaccggct gtgcggctcg
//
LOCUS       DASUCJ010001301         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1159740, whole genome shotgun sequence.
ACCESSION   DASUCJ010001301 DASUCJ010000000
VERSION     DASUCJ010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159740"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFU90_15215"
     CDS             complement(<1..712)
                     /locus_tag="VFU90_15215"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEU5481193.1"
                     /translation="MPAPFCRDTFRDLGPAARKVLAHMHLVERPVRSIVRAVADAAAR
                     WADADFPPRVRALDAVCARTGYSAPVAEYAFDRLFGSLTAAAIEATIVAELGSLAVLD
                     DFVARADGLRARALPAGRVCVISSRTTVGVALTPAVFALCAKCDVVVKDREDALIGAF
                     FRTLAEEDPPFAEAAIARSWDGERDGADLGRYAAIVAFGDDATLAGIRRTLPIGTRLI
                     GFGAKASCGYVGRDALRDV"
     gene            711..1946
                     /locus_tag="VFU90_15220"
     CDS             711..1946
                     /locus_tag="VFU90_15220"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEU5481194.1"
                     /translation="MSWLVRAFFLLVAGAALAGLIGSAACTRRGKSSPLPPTSSPLTT
                     IDVNPKTGSDTTGNGTPDKPYKTLTKAIAVVKSSATTGLTIQLSVGTYDAANGEVFPI
                     VIPTSVSIAGTGYGTRSFRTSGSFINGAGEDTAYEKLLGRPNSRKDFATIEVAQSVTS
                     GASVSGVYVGADRLGLPAGGAYAAADTLGSLVASRVTFAAGTPLSHPAVAGLLVAGGS
                     LNCTACTILGSDYALLALTPPNGTAPSIVLTGQPTQSLIGGTIGIDTDGSASIDASYQ
                     TFQSRRYGYRDAVAPIVSPVPLAGVVDFGHGPTQSAGGNIFIGAASMLSEISVTVAQT
                     LVYAFGDTWNPHTQGTNAQGLYPKARTFRPGDSGRNVTIAPNAAGAAVRVGPIPPPTP
                     TPAPSPTPSGGPTPTPTPT"
     gene            complement(1933..>2308)
                     /locus_tag="VFU90_15225"
     CDS             complement(1933..>2308)
                     /locus_tag="VFU90_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481195.1"
                     /translation="VELGAIVAEYGMTELTSQYYDDVLLRGGSPPDGVRTKVAPPWLR
                     TRVVGPDGATLAGGVVGALVHVDLANRASCVAVSTEDLGVCVERSAGNPQRGIVLIGR
                     EAGADLRGCSLDAEALRGLTSA"
BASE COUNT          386 a          811 c          790 g          321 t
ORIGIN      
        1 ccacgtcgcg cagcgcgtcg cgcccgacgt acccgcaact cgccttcgcg ccgaatccaa
       61 tcaaacgcgt tccgatcgga agcgtgcggc gaatgccggc gagggtggcg tcgtcgccga
      121 acgcgacgat cgcggcgtag cgccccaggt cggctccgtc gcgctcgccg tcccacgagc
      181 gcgcgatcgc ggcctcggca aacggcggat cttcctcggc aagcgtccgg aagaacgcgc
      241 cgatcagcgc gtcctcgcgg tctttcacga cgacgtcgca cttcgcgcac aaggcgaaga
      301 cggcgggcgt cagcgcgacg ccgacggtcg tgcggctcga gatcacgcac acgcgtccgg
      361 ccggcagcgc ccgtgcgcgc aagccgtccg cgcgcgcaac gaaatcgtcg agcaccgcca
      421 gcgaaccgag ctcggcgacg atcgttgctt cgattgcggc ggccgtcagc gatccgaaca
      481 gccgatcgaa ggcgtactcg gcgaccggtg cggaatagcc ggtgcgcgcg cagacggcgt
      541 cgagtgcgcg gacgcgcggc ggaaaatcgg catcggccca gcgcgcggcg gcgtccgcga
      601 ccgcgcgcac gatcgaacga accgggcgct cgacgagatg catgtgcgcg agaacctttc
      661 gcgctgcggg gccgaggtcc cggaaggtgt cgcggcaaaa gggcgcaggc atgtcgtggc
      721 tcgttcgcgc gttcttcctg ctggtggccg gcgcggcgct tgccggcctc atcgggagcg
      781 ctgcgtgcac gcggcgcggc aagtcgtcgc cgcttccgcc gacgtcgtcg ccgctgacga
      841 ccatcgacgt caatccgaaa accgggagcg acaccaccgg taacgggacg cccgacaagc
      901 cgtacaaaac gctgaccaag gcgattgcgg tcgtaaagtc ctcggcgacg acggggctta
      961 cgatccagct ttcggtcgga acctacgacg ccgccaacgg cgaagtcttt ccgatcgtca
     1021 tcccgacgag cgtgtcgatc gcgggcaccg gctacggcac ccggagtttc cggacgagtg
     1081 gatcgttcat taacggtgcc ggtgaagaca cggcctacga aaagctgctc ggccggccga
     1141 actccagaaa agacttcgcg acgatcgagg tagcgcaaag cgtcacttcc ggcgcatccg
     1201 tgagcggcgt ctacgtcggg gcggatcggc tcggattgcc ggccggcggc gcctacgcgg
     1261 cggcggacac gctgggatcg ctggtagcgt cgcgcgtcac cttcgcggcc gggacgccgc
     1321 tctcgcatcc ggcggtcgcc gggttgctgg ttgccggcgg cagcctgaac tgcacggcct
     1381 gcacgatcct cggcagcgat tacgcgctgc tcgcgctcac gccgccgaac ggtacggcac
     1441 cgagcatcgt gttgacggga cagccgaccc agagcctgat cgggggaacg atcggcatag
     1501 acaccgacgg cagtgccagc atcgacgcgt cgtaccaaac gtttcaatcg aggcggtacg
     1561 gctatcgcga cgccgtcgcc ccaatcgtct cgcccgtgcc gcttgcggga gtcgtcgact
     1621 tcggtcacgg cccgacgcag tcggcgggcg gaaacatctt catcggcgcc gccagcatgc
     1681 tgtcggagat ctcggtaacg gtggcgcaga cgctggtgta cgcgttcggc gatacgtgga
     1741 atccgcacac gcaaggaacg aacgcgcagg gactgtatcc gaaggcgcgc acgttccgcc
     1801 ccggcgattc gggacgcaac gtcacgatcg cacccaatgc ggccggcgcg gcggtgcgcg
     1861 tgggtccgat tccgccgccg acgccgacgc ccgcaccgag cccgacgccg agcggcggtc
     1921 ccacccccac gcctacgccg acgtaaggcc gcgcagcgct tcggcgtcga gcgaacagcc
     1981 gcgcaagtcc gcgccggcct cacgtccgat cagcacgatt cctctttgcg gatttccggc
     2041 ggagcgttcg acgcaaacgc cgagatcttc ggtcgacacg gccacgcacg acgcgcggtt
     2101 cgcgaggtcg acgtgcacga gcgcgccgac gacgccgccg gcgagggtcg cgccgtcggg
     2161 ccctacgacg cgcgtgcgca gccacggcgg ggcaaccttc gtgcgtacgc cgtccggcgg
     2221 cgatccgccg cgcagcagca cgtcgtcgta gtattgcgac gtaagctccg tcatcccgta
     2281 ttcggcgacg atcgcgccaa gttcgacg
//
LOCUS       DASUCJ010001302         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1374017, whole genome shotgun sequence.
ACCESSION   DASUCJ010001302 DASUCJ010000000
VERSION     DASUCJ010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374017"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1560)
                     /locus_tag="VFU90_15230"
     CDS             complement(<1..>1560)
                     /locus_tag="VFU90_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5481196.1"
                     /translation="YWFYWAAGRKTRPEDHSAHGAYTWKDYFRINTDHKVIGIQYTVS
                     SFFFLLVGGLIAMLMRAELAAPGAQFVDANTYNGLFSVHASLLIFLFVIPVFAGLGNF
                     VIPLMIGAPDMAFPRLNALSFWLLPVAGALMIGSFFAPGGAFADGWTAYAPLSTDAPV
                     GQTFFSLAVQFAGASSIMTALNFLVTIITMRAPGMSFFRMPLLVWANFATSLLVVIAT
                     PFIAASQFFVLLDRALGFNFFNAAKGGDVLMYQHVFWFYSHPAVYIMMLPGFGIISEV
                     IAVKARKPIFGYRMMAFSLLAIVGLGFTVWAHHMFTSGMAGWIRIPLAITTAIIAVPT
                     GIKIFSWLATLWRGVLRLDTPMLFALGFLTTFTLGGISGVMLAMIPFDIHVSATYFIV
                     AHIHYVLFGGSLMTIFAGVYYWFPKMTGRMFDERLGKIHFWATFVFFNLTFAPMHILG
                     LQGMPRRVSTYASEFAGLNLFISIASFGLGLSTLIFVYNMVTSWRGGPRAVANPWRGM
                     TLEWQVSSPPP"
BASE COUNT          325 a          473 c          551 g          211 t
ORIGIN      
        1 ccggcggggg cgaggagacc tgccactcga gcgtcatgcc gcgccacggg ttggccacgg
       61 cgcgcggccc gccgcgccag ctcgtgacca tgttgtagac gaagatcagc gtgctcagcc
      121 cgagcccgaa gctggcgatc gagatgaaca ggttcaggcc cgcgaattcg gaggcgtagg
      181 tcgagacccg gcgcggcatg ccctgcagac cgaggatgtg catcggcgcg aacgtgaggt
      241 tgaagaacac gaacgtagcc cagaagtgga tcttgcccag gcgctcgtcg aacatccgcc
      301 cggtcatctt tgggaaccag tagtagacgc cggcgaagat cgtcatcagc gagccaccga
      361 acagcacgta gtggatgtgg gcgacgatga agtaggtcgc gctgacgtgg atgtcgaacg
      421 ggatcatcgc gagcatcacg ccgctgatcc cgccgagggt gaacgtggtg aggaagccca
      481 gcgcgaacag catcggcgtg tccaggcgca gcacccctcg ccacagcgtc gccagccacg
      541 agaagatctt gatgccggtc ggcacggcga tgatcgcggt cgtgatcgcc aacgggatgc
      601 ggatccagcc cgccatcccc gacgtgaaca tgtggtgcgc ccagacggtg aagccgaggc
      661 cgacgatcgc gagcagcgag aacgccatca tgcggtagcc gaagatcggc ttgcgcgcct
      721 tcacggcgat gacttcgctg atgatcccga agcccggcag catcatgatg tagacggccg
      781 ggtgggagta gaaccagaag acgtgctggt acatcagcac gtcgccgccc ttcgccgcgt
      841 tgaagaagtt gaagccgagc gcgcggtcga gcagcacgaa gaactgcgac gcggcgatga
      901 acggcgtcgc gatcacgacg agcagcgacg tcgcgaagtt ggcccacacg agcagcggca
      961 tccggaagaa gctcatgccc ggcgcgcgca tcgtgatgat cgtgacgagg aagttgagcg
     1021 cggtcatgat cgacgaggcg ccggcgaact gcacggcgag cgagaagaac gtctggccga
     1081 cgggcgcgtc ggtcgacagc ggcgcgtatg ccgtccagcc gtcggcgaac gcgccgccgg
     1141 gcgcgaagaa gctgccgatc atcagcgcgc cggcgaccgg cagcagccag aacgacagcg
     1201 cgttcaggcg cgggaacgcc atgtcgggcg cgccgatcat cagcgggatc acgaagttgc
     1261 cgagacccgc gaagacgggg atcacgaaca ggaagatcaa gagcgacgcg tggaccgaga
     1321 agaggccgtt gtaggtgttc gcgtcgacga actgcgcgcc cggcgcggcg agctcggcgc
     1381 gcatcagcat cgcgatcagc ccgccgacga gcaggaagaa gaacgagctg accgtgtact
     1441 ggatgccgat caccttgtga tcggtgttga tccggaagta gtccttccac gtgtacgcgc
     1501 cgtgcgccga gtggtcctcg gggcgcgtct tgcggccggc ggcccagtag aaccagtagt
//
LOCUS       DASUCJ010001303         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_777904, whole genome shotgun sequence.
ACCESSION   DASUCJ010001303 DASUCJ010000000
VERSION     DASUCJ010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777904"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2494)
                     /gene="acnA"
                     /locus_tag="VFU90_15235"
     CDS             complement(<1..2494)
                     /gene="acnA"
                     /locus_tag="VFU90_15235"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5481197.1"
                     /translation="MNQNVNSFNAIESLRVGDRTYTYFSLNALAGLTDLARLPFSLKI
                     LLENLLRFEDGRSVTKHDVESLARWSPTARHEKEIAFRPARVLLQDFTGVPCVVDLAA
                     MRDAMADLGGDPKAINPLQPVELVVDHSVQIDFFGSAQALAKNAELEFQRNHERYAFL
                     KWGQSAFAGFRAVPPDTGIVHQVNIEYLARVVFGAEDTAANPLAYPDTAVGTDSHTTM
                     VDGLGVLAWGVGGIEAEAAMLGQPVTMLIPEVVGVRLEGALREGVTATDLVLTVTEQL
                     RKKGVVGKFVEFFGPGLSALPVADRTTLGNMSPEFGSTVAMFPIDDRTLDYLRLTGRS
                     SERIALVEAYAKAQGLFRTDATPDPEFSDVLRLNLSDVEPSLAGPRRPQDRVPLHDVK
                     RSWASALEQWLDARDASNTAIARFEGEGGGGTATIAETEKNVSDGAVVIAAITSCTNT
                     SNPSVLIGAGLLARNAVRLGLHTQPWVKTSLAPGSKVVTDYLAKSGLQEYLDRLGFNV
                     VGYGCTTCIGNSGPLPPDIAETVADQQLIVAAVLSGNRNFEGRIHPQVHANYLASPPL
                     VVAYALAGRMDVDLTSEPLGTGSDGKPVYLKDVWPGDDEISETIARCVDDAMFKARYA
                     DAFRGDDNWAKLTVEPSERYAWDPQSEYVKKPPYFEGMGIEPPPLRDVRGARVLAVLG
                     DSVTTDHISPAGNIAKISPAAKYLMERGIEPKDFNSYGSRRGNHEVMVRGTFANVRLR
                     NHLVPGVEGGFTRYLPTGEQMPIFDAAVKYQADGTPLIVLAGREYGSGSSRDWAAKGP
                     YLLGIKAVIADSFERIHRSNLIGMGVLPLEYVD"
     gene            complement(2475..3665)
                     /locus_tag="VFU90_15240"
     CDS             complement(2475..3665)
                     /locus_tag="VFU90_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEU5481198.1"
                     /translation="MTVPIVVNDSDSLKMLCTRVRSAERVALDTEFHAERTYAPRLMV
                     VQIAFDDGAAIVDPLALPDLRALADALAEPTVVGHALSSDLKIFADRFGTVPARVFDT
                     QIAAAFLGYGMQVSLADLVRDLRRVRLAKSQTVSDWSSRPFSDRQLEYLVDDVAHLLP
                     MHDVLRERLEVKGRYDWAIEECAELGEIGRYRFDERRSYLRIPGAMRMSRRELGILNE
                     LVKLRDRVARERDLPLKYVMPDDVVAGLATLKPKRVEDLSQLRRLDAGARRQLGDAIL
                     QAVARGEALPEDALPERPQRPLGPARDTLAALLAVVAGEIARDAGLPTNLLVPRAALE
                     RVAREVPRDRESFDAALALQPWRLALAGDSLWQLLSGERVLSVEGYAGGNPKICLSHE
                     SERQ"
     gene            complement(3662..>4024)
                     /locus_tag="VFU90_15245"
     CDS             complement(3662..>4024)
                     /locus_tag="VFU90_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481199.1"
                     /translation="LLDLHDYLQRLPGWVKRFPVGSPAFLIAVRVIPGFGGTVATASA
                     AAFRVPLWVHVWTMCAIAVPLCVILTIFGDRVTVFVHGVTHRYEDRYQRYCRTHRCPH
                     FRFQHFRFHREVEVSPAP"
BASE COUNT          711 a         1333 c         1311 g          669 t
ORIGIN      
        1 gatcgacgta ttccagcggc agcacgccca ttccgatcag attgctgcgg tgaatccgtt
       61 cgaacgaatc ggcgatcacc gccttgatgc cgagcaggta cggccccttc gccgcccagt
      121 cgcgcgacga acccgaaccg tattcccgac cggccaacac gatcagcggg gtcccgtcgg
      181 cctggtactt cacggccgca tcgaagatcg gcatttgctc gccggtcgga agatagcgcg
      241 tgaagccgcc ttcgaccccc gggacgagat ggttgcgcag gcgcacgttg gcgaacgtgc
      301 cgcgcaccat aacctcgtgg ttgccgcgcc gcgagccgta cgagttgaag tctttgggct
      361 cgatgccgcg ctccatcagg tatttcgcgg ccggactgat cttcgcgatg ttgccggccg
      421 gcgatatgtg atcggtggtc accgagtcgc cgagcaccgc gaggacgcgc gcgccgcgaa
      481 cgtctctcag cggcggcggc tcgattccca taccttcgaa gtacggcggt ttcttgacgt
      541 actcggactg cggatcccag gcgtagcgct cgctgggctc gaccgtcagc ttggcccagt
      601 tgtcgtcgcc gcgaaacgca tcggcgtatc gcgccttgaa catcgcgtcg tcgacgcagc
      661 gcgcgatcgt ctcggagatc tcgtcgtcgc ccggccaaac gtccttgaga tacaccggtt
      721 tgccgtcgct gccggtcccg agcggctcgc tcgtcaaatc cacgtccatc cgcccggcga
      781 gcgcgtacgc gacgaccagc ggcggcgagg ccaaatagtt ggcgtgcact tgcggatgaa
      841 tccgcccctc gaagttccgg ttgcccgaaa gcacggcggc gacgatcagc tgctggtcgg
      901 cgaccgtttc ggcgatgtcg ggcgggagcg ggccggagtt tccgatgcac gtggtgcagc
      961 cgtaccccac gacgttgaag ccgagccggt cgaggtactc ctgtaagccg gatttggcga
     1021 gatagtcggt gacgactttc gaacccgggg cgagcgacgt tttcacccac ggctgcgtgt
     1081 gcagtccgag tcgcaccgca ttgcgcgcca acaggccggc gccgatcagc accgacgggt
     1141 tggacgtgtt ggtgcagctg gtgatcgcgg cgatcaccac cgcgccgtcg gagacgttct
     1201 tttcggtctc ggcgatggtc gcggttcccc cgccgccttc accttcgaaa cgcgcgatcg
     1261 cggtgttgct cgcatcgcgc gcgtcgagcc attgctcgag tgcggatgcc cacgaacgct
     1321 tcacgtcgtg cagcgggacc cggtcctgcg gccggcgcgg gcccgccaaa ctcggctcga
     1381 cgtccgaaag attcagacgg agcacgtcgc tgaactcggg atccggcgtc gcgtcggttc
     1441 ggaacaaacc ttgcgccttg gcgtacgctt cgaccagtgc gatccgctcg gacgagcggc
     1501 cggtcaagcg cagatagtcg agcgtgcgat cgtcgatcgg aaacatcgca acggtcgaac
     1561 cgaactccgg cgacatgttc ccgagcgtgg tgcgatcggc aaccggcaac gccgaaagcc
     1621 ccggtccgaa gaactcgacg aatttcccga cgacgccctt cttgcgcagc tgttcggtaa
     1681 cggtgagcac gagatcggtt gcggtcacac cctcccgcag cgcgccttcg agccgaacgc
     1741 cgaccacctc gggtatcagc atcgtaaccg gctgcccgag catcgccgct tcggcttcga
     1801 ttccgccgac gccccaggcg agcacgccga gcccgtcgac catcgtcgtg tgcgagtcgg
     1861 ttccgacggc cgtgtcggga taggccagcg gattcgccgc cgtatcctcg gcgccgaaaa
     1921 ccacgcgcgc gagatactcg atgttcactt ggtggacgat gccggtgtcg ggcggcaccg
     1981 cgcgaaagcc ggcaaaggcc gattgccccc acttcagaaa cgcgtaccgc tcgtggttgc
     2041 gctgaaattc cagttcggcg ttcttggcga gcgcttgcgc ggatccgaag aagtcgatct
     2101 gcaccgagtg gtcgacgacc agttcgaccg gctgcaacgg attgatcgcc ttcggatcgc
     2161 cgcccagatc ggccatcgcg tcgcgcatcg ccgcgagatc gaccacgcac gggacgccgg
     2221 tgaaatcctg aagcagcacg cgcgccggcc ggaacgcgat ttctttttcg tgtcgcgccg
     2281 tcgggctcca gcgcgccagg ctttccacgt cgtgcttcgt aaccgatcgg ccgtcttcga
     2341 agcgcaataa attttccaac aggatcttca aggaaaacgg cagccgggcc aaatccgtca
     2401 acccggcgag tgcgttcagg ctaaagtacg tgtaggtacg atcgccgacg cgcagcgatt
     2461 cgatcgcgtt gaagctattg acgttctgat tcatgggata ggcatatttt cggattaccg
     2521 cccgcgtagc cttcgacgct caggaccctt tcgcccgata gcagctgcca caaggaatcg
     2581 ccggccagcg cgaggcgcca cggctgcagc gcgagcgcgg catcgaaact ctcgcgatcc
     2641 cgcggcactt cgcgcgcaac gcgctccagg gcggcgcgcg gtaccagcag gttcgtcggc
     2701 agacccgcat cgcgcgcgat ttcgccggcc acgacggcca gcagcgccgc cagcgtatcg
     2761 cgcgccgggc cgagcggccg ctggggacgt tcgggcagag cgtcttcggg gagcgcctcg
     2821 ccgcgcgcca ccgcctgcag gatggcgtcg cccagctgcc ggcgggcgcc ggcgtccaaa
     2881 cggcgcagct gcgagaggtc ctcgacccgt ttgggtttga gcgtcgcaag cccggcgacg
     2941 acgtcgtcgg gcatgacgta cttcagcggc agatcgcgtt cgcgcgctac gcggtcgcgc
     3001 agcttcacga gttcgttgag aatcccgagc tcgcgccggc tcatgcgcat cgcgccggga
     3061 atccgaagat aggagcgccg ttcgtcgaag cgatagcggc cgatttcgcc cagttcggcg
     3121 cactcctcga tcgcccagtc gtatcgccct ttgacttcga ggcgctcgcg taagacgtcg
     3181 tgcatcggaa gcagatgcgc gacgtcgtcg accaggtatt cgagctgccg gtccgagaac
     3241 gggcgcgacg accaatcgga gacggtctgc gatttcgcca gccggacgcg gcgcaggtcg
     3301 cgcaccaggt cggcgagcga gacctgcatt ccgtatccca agaacgcggc cgcaatctgc
     3361 gtgtcgaaca cgcgcgcggg caccgtgccg aagcgatcgg caaagatctt caagtcggaa
     3421 gaaagcgcat gccccaccac ggtgggttcg gcaagggcgt ccgccagcgc gcgcaaatcg
     3481 ggcagcgcca gcggatcgac gatcgccgcg ccgtcgtcga aagcgatctg cacgaccatc
     3541 aggcgcggcg cgtaggtgcg ttccgcgtgg aactcggtgt ccagcgcgac gcgttcggcg
     3601 ctccgcacgc gcgtgcagag cattttgaga gaatcggagt cgttgacgac gatcggtacg
     3661 gtcatggagc cggtgaaacc tcaacctccc ggtggaagcg gaagtgctgg aagcggaagt
     3721 gcgggcaacg gtgggtacgg cagtaacgct ggtaccggtc ttcgtaacga tgcgtcacgc
     3781 cgtgcacgaa aacggtcacg cgatcgccga aaatcgtcag aatcacgcac agcggtacgg
     3841 cgatcgcgca catcgtccag acgtgcaccc acaacggcac tcgaaacgcc gcggcgctgg
     3901 ccgtcgccac cgtcccgccg aacccgggga ttacgcgaac ggcgatcagg aaggccggag
     3961 agccgaccgg aaaccgtttt acccatccgg gcaggcgctg caggtagtcg tgcagatcca
     4021 gcag
//
LOCUS       DASUCJ010001304         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_885147, whole genome shotgun sequence.
ACCESSION   DASUCJ010001304 DASUCJ010000000
VERSION     DASUCJ010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885147"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1137)
                     /locus_tag="VFU90_15250"
     CDS             complement(94..>1137)
                     /locus_tag="VFU90_15250"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5481200.1"
                     /translation="PAFTVSRACASANQAITDAADQIVLGHADVAIAGGAESLSNVPI
                     LHSRRFSDVLVRASKAKSLGARVSAFSHVRPRDLVPVTPAIAEPSTGETMGQSAEKMA
                     KLNHIPREEQDQFALRSHRLAAAGTQDGRLTSEIMPVYVSPAYEAWAATDNGVRTDTS
                     LEQLAALKPVFDRRYGSVTAGNSSPLTDGGAAVILMSEEKARSLGMQPIAYLRSYAYA
                     ALDPGEQLLQAPVFAAPKALERAGLTLANIDLVEMHEAFAAQVLSNLQGFESTAWAER
                     AGLSQPMGEVDRSRLNVMGGSIAIGHPFGATGARITTTLAHELKRRGGQFGLMTVCAA
                     GGMGFAMVLENAA"
BASE COUNT          180 a          431 c          352 g          174 t
ORIGIN      
        1 acgcgtcggg aacgtcgagc gtcgcgaccg caatgccgtt ctggatgtcg accgcgaacg
       61 cggacgccgc ttgcaccgtg tccgtcgcgg cgctcacgcg gcgttctcca acaccatcgc
      121 gaatcccatt ccgccggccg cgcacacggt catcagcccg aactgccccc cgcgccgctt
      181 gagctcgtgc gccagcgtcg tcgtgatacg cgccccggtg gcgccgaacg ggtggccgat
      241 ggcgatcgag ccgcccatga cgttgaggcg cgatcggtcc acttcaccca tcggctgcga
      301 caggccggcg cgttccgccc acgccgtgct ctcgaatcct tgcagattgg acaacacctg
      361 ggccgcgaac gcctcgtgca tctcgaccag gtcgatgttg gccagcgtga ggccggcgcg
      421 ctcgagcgcc ttcggagccg caaacaccgg cgcctgcaac aactgctcgc ccgggtccag
      481 cgccgcgtac gcgtacgatc gaaggtacgc gatcggctgc atgcctaacg aacgagcctt
      541 ctcctcgctc atgagaatca cggccgcgcc gccgtcggtg agcggcgacg aattgccggc
      601 cgtcaccgag ccgtagcgcc ggtcgaacac cggcttgagc gccgccagct gctcgagact
      661 cgtgtccgtc cgcaccccgt tgtcggtcgc cgcccacgcc tcgtacgccg gcgacacgta
      721 caccggcatg atctccgacg ttaggcgacc gtcctgcgtc cccgccgccg ccagccggtg
      781 cgaccgcagc gcgaactggt cctgctcctc gcgaggaata tggttgagct tggccatctt
      841 ctccgccgac tgccccatcg tctcacccgt cgatggctcg gcaatcgctg gcgtcaccgg
      901 caccaggtcg cgcggacgca cgtgcgagaa cgcgctcacc cgagcgccca acgacttcgc
      961 cttactcgcg cgcacgagca catccgaaaa ccggcgcgaa tggagaatcg gcacgttcga
     1021 cagcgactcg gcgcctccgg caatggccac gtcggcgtga ccgagcacga tctggtccgc
     1081 tgcgtcggtg atcgcctggt tcgcggaagc acacgcccgg ctcaccgtga acgccgg
//
LOCUS       DASUCJ010001305         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1267158, whole genome shotgun sequence.
ACCESSION   DASUCJ010001305 DASUCJ010000000
VERSION     DASUCJ010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267158"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFU90_15255"
     CDS             <1..284
                     /locus_tag="VFU90_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481201.1"
                     /translation="FSGKPVKPLPTPKPIPTGCNTANLIGTWSSDDKPGLTLQMTIDG
                     SLTDSDGVSGNWGLYNTTATLTYYGTHTMTLSSDGKHLRGGGYNFTRKC"
     gene            295..663
                     /locus_tag="VFU90_15260"
     CDS             295..663
                     /locus_tag="VFU90_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5481202.1"
                     /translation="MTEWKPPGWHSLTPRLVADDPAGLVEFLRRAFGARGEYRADRPS
                     QLRIGDSIVMVGGAGVRERIRSFLYLYVEDADATYRRALDAGAESLEEPVDTPYGDRR
                     AMIADPCGNRWQIATYRGEA"
     gene            752..1138
                     /locus_tag="VFU90_15265"
     CDS             752..1138
                     /locus_tag="VFU90_15265"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU5481203.1"
                     /translation="MPRKKSLVLTDHELRLMEVLWRRRRATVAGVVEALPPPPLAYST
                     VLTTLRTLEQKGYIAHEEDGRAYVYRPVVAREQAARSAMNYLLDRFFGNSPGALAVTL
                     LDDSRLSDDDVAKIKRMLARSRKAPR"
     gene            1135..2343
                     /locus_tag="VFU90_15270"
     CDS             1135..2343
                     /locus_tag="VFU90_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5481204.1"
                     /translation="MIDTILLNGLWQGLPIVAVAAAISALLPRKHAATRYAVWFTALL
                     ALALVPVVSVVHLGRPIAPLPPSIVHTAAATSIVETQAANAGGLWLALFWLTGVAFCC
                     ARLSISHVRIARIVRRAVPAPELGSDVMTSGDVGVPIAARIASPVILIPRSLASTLDR
                     GDLESIVQHERAHIRRFDLAGNLIQRFVEALLFLNPWAYVIGRQLVKEREFACDDWAA
                     NVTGEPDRLAACLGRLAESAHRSRVPLFTPSAIGSKPMIVGRIARLLDGKAAAVKTNY
                     FVVLSAVLALGALAIALQTSNGLASVGNAVAANNPPASKCYADVKPLEAAMPNIPKAD
                     YQPKLSANALVTVASNGRPTDAKIVKSSGNAAVDRATTEAAMASTYSPKMVDCKATTG
                     QYLFQVDTGP"
     gene            complement(2344..>2970)
                     /locus_tag="VFU90_15275"
     CDS             complement(2344..>2970)
                     /locus_tag="VFU90_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481205.1"
                     /translation="LVIGGALLLARGTVRIGAVVLALIYFIFAVFWMPRLITAPQVLG
                     YHVAVYAGVLDGVCQQLILAAAAALPYAASLGDAFRARTIATVARWVFGLSSIDFGLA
                     HWLGMAGTARLVPAWLPLGGNFWTGLTGICFLLAGIAILIGLLDALAAWLLGAMLLVF
                     SAIVLAPMIFEFPRVQNAWASNGYNLAAVGAAWVLAGYLSARKNRGAA"
BASE COUNT          554 a         1022 c          914 g          480 t
ORIGIN      
        1 acttctcggg caagcccgtc aagccgctgc ccacgcccaa accgattccg accggctgca
       61 acaccgccaa tctcatcggc acctggtcgt ccgacgacaa gccggggctc acgctgcaaa
      121 tgacgatcga tggatcgctg accgacagcg acggcgtctc cggcaactgg ggattgtaca
      181 acacgaccgc aaccctgacg tattacggga cgcacacgat gacgctctcg tcggacggca
      241 agcatctccg cggcggaggc tacaacttca cgcgtaagtg ctgacgccgc ttacgtgacg
      301 gaatggaaac cgccgggctg gcatagcctg acgccgcgac tcgttgccga cgatcccgcc
      361 ggactcgtcg agttcttacg gcgcgctttc ggcgcacggg gcgagtaccg cgccgaccgg
      421 ccgtcgcagc tgcgcatcgg ggattcgatc gtgatggtcg gcggcgccgg cgtgcgcgag
      481 cgaattcgca gctttctcta tctctacgtc gaggacgccg acgcgacgta ccggcgcgcg
      541 ctcgacgcgg gcgcggagtc gctcgaggaa ccggtcgata cgccgtacgg cgatcgccgc
      601 gcaatgattg ccgatccgtg cggcaaccgt tggcaaatcg ccacctatcg cggcgaagcc
      661 tagccgttta ccattagaaa actagttgac aaagccggcg gtgccggcta gagttagtat
      721 tagtttccta atagtacaac ccggaggtcg cgtgccccgc aagaaatcgc tcgttctcac
      781 cgatcacgaa ctccgtttga tggaggtact gtggcgacgg cgccgggcga cggtcgccgg
      841 cgtggtcgag gcgcttccgc ccccgccgct cgcctatagc acggtgctca ccactttgcg
      901 cacgctcgaa cagaaggggt acatcgctca cgaggaagac ggacgcgcgt acgtctaccg
      961 tcccgtagtc gcgcgcgagc aagcggcgcg ctcggcgatg aattatttgc tcgatcgctt
     1021 cttcggaaat tcgccgggcg ccctcgcggt cacgctgctg gacgactcgc ggctgagcga
     1081 cgacgacgtc gcaaaaatca aacgaatgct cgcgcgcagt cgaaaggcgc cgcgatgatc
     1141 gacacgattc tcctcaacgg tctttggcag gggctgccga tcgtcgccgt ggcggcggcg
     1201 atctcggcac tgctgccccg gaagcacgcc gccacgcggt acgccgtgtg gtttaccgcg
     1261 ttgctcgcgc tcgcgctcgt gccggtcgtc agcgtcgtgc atctcgggcg cccgatcgcg
     1321 ccgctcccgc cgtccatcgt gcacaccgcc gcggcaacct cgatcgtcga gacccaggcc
     1381 gcgaacgccg gcggtctgtg gcttgcgctg ttttggctaa ccggcgtcgc gttctgctgc
     1441 gcacggctgt cgatcagcca cgtgcggatc gcgcgaatcg ttcgtcgcgc cgtgccggca
     1501 cccgaattgg gcagcgacgt catgacgtcc ggcgacgtcg gcgttccgat tgccgcgcgc
     1561 atcgcatcgc cggtgatcct gattccacgt tcgctcgcct cgacgctcga tcgcggcgac
     1621 ctcgaaagca tcgtgcagca cgagcgggcg cacattcgcc ggttcgacct ggccggcaac
     1681 ctcattcagc gcttcgtcga agccttgctc ttcttaaacc cctgggcgta cgtcatcggc
     1741 cggcaactcg tcaaagagcg cgaattcgcc tgcgacgact gggccgccaa cgtcaccggc
     1801 gaacccgatc gcttagcggc ttgtctcggc cggttggccg aaagcgcgca tcgctcgcgc
     1861 gttcctctat ttacccccag cgcgatcggc tcgaagccca tgatcgtggg acgcatcgcg
     1921 cgcttactcg atggaaaggc agctgccgtg aagaccaact acttcgtcgt tctgtcggcc
     1981 gtgctcgcgc tcggtgctct cgccattgca ttacagacca gcaacggact ggcttcggtc
     2041 ggcaatgcgg tcgccgcgaa taacccgcct gcctcgaagt gctacgccga cgtcaagccg
     2101 ctcgaggccg ccatgcccaa catccccaaa gcggattatc agccgaaact gtcggcgaac
     2161 gcgctcgtca cggtcgcctc caacgggcgg cctaccgatg cgaagatcgt caagtcgagc
     2221 gggaacgccg ccgtcgatcg cgcgaccacc gaggcggcga tggcctcgac ctattcaccc
     2281 aagatggtgg attgcaaagc gacgaccggg cagtatctct tccaggtcga caccgggccg
     2341 taactacgcg gcccctcgat tcttgcgggc ggagaggtat ccggccagca cccacgccgc
     2401 tccgacggca gccaggttgt aaccgttgct ggcccacgcg ttctgaacgc gcggaaattc
     2461 aaagatcatc ggagccagca cgatcgcgct gaatacgagc agcatcgcac ccagcagcca
     2521 cgcggcgagt gcgtcgagca acccgatcag aatcgcgatg ccggccagaa gaaaacagat
     2581 tccggtcaag cccgtccaaa agttaccgcc gagcggcagc cacgccggaa cgaggcgcgc
     2641 ggtaccggcc atcccgagcc agtgcgctaa cccgaagtcg atcgacgaaa ggccgaaaac
     2701 ccaccgcgcg accgttgcga tcgtccgcgc ccggaatgcg tcgccgagcg acgcggcgta
     2761 cggcagcgcc gcggccgccg ccaagatcag ctgctggcag acgccgtcca gcacgccggc
     2821 gtagacggcg acgtgataac ccagcacctg cggcgccgta atcagccgcg gcatccaaaa
     2881 cactgcaaag atgaaataga tcagcgcgag gacgacggct ccgatgcgaa ccgtcccgcg
     2941 tgcgagcagc agcgccccgc cgatgacgag
//
LOCUS       DASUCJ010001306         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1389683, whole genome shotgun sequence.
ACCESSION   DASUCJ010001306 DASUCJ010000000
VERSION     DASUCJ010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389683"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFU90_15280"
     CDS             <1..459
                     /locus_tag="VFU90_15280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5481206.1"
                     /translation="MTDAVSLVFDDAEGATELDTDDARVAQILRNLLSNAIKFTERGE
                     IRVSAAVEVGDLIRFDVRDTGIGIAQADQERIFDEFTQIDSHLQRRVHGSGLGLRLSR
                     NLATLLGGRIELVSAPGRGSTFSVIIPRRHPGWATQLAPNEATPHEVTVP"
     gene            456..>1284
                     /locus_tag="VFU90_15285"
     CDS             456..>1284
                     /locus_tag="VFU90_15285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5481207.1"
                     /translation="MTTHAESGLPIDRIPADAPPAPRDGEKPAVRAHDSVDDLPDAMA
                     ESSTPSVLVVDDTPGNRYTVVRVLRGAGMRVTEAETGAEALRFADALPDLIVLDIRLP
                     DMTGYDVCQRLKTDPETKSIPVMYVSASYTGNADRAYGLDAGADAYLTHPIDAPLFLA
                     TAHALLRAGRAEAAFRRAAVEWRTTFDAIRDAIFLVDAEGIVRRCNRAGGELARQHPR
                     TLVGQQWSQLVHDLGVDSPDTLNGVVRGPVLHDVEVGQRGRWLNVSTDRVVGHVPEQS
                     "
BASE COUNT          216 a          422 c          436 g          210 t
ORIGIN      
        1 atgacggatg cagtcagcct cgtgttcgat gacgcggaag gcgcgactga gctcgatacc
       61 gacgacgcgc gtgtcgcgca gattctgcgc aacctgcttt cgaacgcgat caagttcacc
      121 gagcgcggcg agattcgcgt gtccgcggcc gtcgaggttg gcgatctgat ccgattcgac
      181 gtgcgcgata ccggcatcgg gattgcgcag gcggaccagg agcgcatttt cgacgagttc
      241 acgcagatcg acagccactt gcagcgccgc gtccacggca gcgggcttgg attgcgcttg
      301 tcgcgaaatc tggcgacgtt gctcggcggt cgcatcgagt tggtcagcgc gccggggcgc
      361 ggatcgacgt tcagcgtgat cattccgcgg cggcaccccg gttgggccac gcagttggcg
      421 cctaacgagg cgacgccgca cgaggtgacg gtgccgtgac gacccacgcg gaaagcggct
      481 tgccgatcga tcgcataccg gccgacgccc ctccggcgcc gcgcgacggc gagaaaccgg
      541 cggtgcgcgc acacgattca gtggacgact tgcccgatgc gatggccgag tcgtccacgc
      601 cttcggtgct tgtcgtggat gacacgccgg gtaaccgata caccgtggtt cgcgtgctcc
      661 gcggcgcggg catgcgcgtg acggaagccg agacgggcgc cgaagcgctt cggttcgcgg
      721 acgcgctacc ggatctcatc gtgctcgaca tcaggctgcc ggacatgacc ggctacgatg
      781 tgtgccaacg gctgaaaaca gacccggaaa cgaaatcgat tcccgtgatg tacgtgtcgg
      841 cgagctacac cgggaacgcc gaccgcgcct acggcctcga cgccggcgcc gacgcatatc
      901 tcacgcaccc gatcgatgca ccgctcttcc tggccacggc gcacgccctg cttcgtgcgg
      961 gacgggcgga ggctgcgttc cgccgcgccg ccgtcgagtg gcgcacgacg ttcgacgcca
     1021 tccgcgacgc gatcttcctc gtggacgccg aggggatcgt acggcgatgc aaccgcgccg
     1081 gcggcgagct cgcgcgccaa catccgcgaa cgctcgttgg acagcagtgg tcacagctcg
     1141 ttcacgactt gggcgtcgac tctcccgata cgctgaacgg cgttgtccga ggaccagtgc
     1201 tccacgacgt cgaagttggg cagcgcggac ggtggctcaa cgtctcgacc gatcgcgttg
     1261 tcggtcacgt gccggagcaa tcgt
//
LOCUS       DASUCJ010001307         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_732277, whole genome shotgun sequence.
ACCESSION   DASUCJ010001307 DASUCJ010000000
VERSION     DASUCJ010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732277"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFU90_15290"
     CDS             <1..695
                     /locus_tag="VFU90_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481208.1"
                     /translation="SPGLCSNVAALDREGYNGRMLLAVVGRERLEKILARALDEAHFF
                     SPHGIRSVSKYHAENPYSLDIDGQIHSVDYEPAESNTGTFGGNSNWRGPVWFPINFLL
                     VEALQKFHYQAGDDFKVECPTGSGKMLTLWEVSMELSHRLVSLFTVDERGRRPIYGEV
                     EKFQSDPHWREYLSFFEYFHGDTGQGLGASHQTGWTGLVAKLIQQHGEYCGRGVHPFA
                     ARTKVTESQVGA"
     gene            692..1342
                     /locus_tag="VFU90_15295"
     CDS             692..1342
                     /locus_tag="VFU90_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5481209.1"
                     /translation="MSVLVVDVGGTHVKILATAQTQRREFASGPELTAAGMVEAVKKL
                     AEGWAYDRVAIGYPGPVLHGMPASEPHNLGTGWVGFDFAAAFGCPVKLVNDAAMQALG
                     SYRGGKMLFLGLGTGLGTTLIVDGVVEPMELAHPPYKKATYEDYVGERALKRFGKKKW
                     SKKVAAVVAALRAAFEPDDVVLGGGNVKKLAELPPGCRAGDNENAFAGGFKLWEPS"
BASE COUNT          247 a          395 c          479 g          221 t
ORIGIN      
        1 actcgccggg actgtgctcg aacgtggcgg cgctcgaccg cgagggctac aacggccgga
       61 tgctgctggc ggtagtgggg cgcgagcggc tcgagaagat cctcgcgcgc gcgctcgacg
      121 aagcgcactt ctttagcccg cacggcattc gctcggtatc gaagtatcat gccgagaatc
      181 cgtactcgct cgacatcgac ggccaaatcc acagcgtgga ttacgagccg gcggaatcga
      241 ataccggaac tttcggcgga aactcgaatt ggcgaggtcc ggtgtggttt ccgatcaact
      301 ttctgctcgt cgaagcgctg cagaagttcc actaccaagc cggcgacgac ttcaaggtcg
      361 agtgcccgac cggatcgggg aagatgctga cgctttggga ggtctcgatg gagctttcgc
      421 accgtctggt gagcctgttt accgtcgacg aacgcggccg gcgtccgatt tacggcgagg
      481 tcgagaagtt tcagagcgat ccgcactggc gcgagtacct ctcgttcttc gaatactttc
      541 acggcgacac ggggcaaggg ttgggcgcga gccatcagac cggctggacg gggctcgtgg
      601 ccaagctgat ccagcagcac ggcgaatatt gcgggcgggg cgttcacccg ttcgcggcgc
      661 gcacgaaggt taccgaatcg caggtggggg cgtgagcgtc ctggtggtcg acgtcggcgg
      721 aacgcacgtc aaaattctgg ccacggcgca aacgcagcgc cgcgagttcg cgtcgggccc
      781 cgagctgacc gccgccggta tggtcgaggc cgtcaagaag cttgccgaag gctgggcgta
      841 cgatcgcgtc gcgatcggat atccgggccc ggtgctgcac ggcatgccgg ccagcgagcc
      901 gcacaatctc ggtacgggct gggtcgggtt cgactttgcc gcggcgttcg gatgcccggt
      961 caaactcgtc aacgacgcgg cgatgcaggc gctggggagt taccgcggcg gcaaaatgct
     1021 gttcctcggg ctgggaacgg gactcggcac gacgctgatc gtcgacgggg tcgtcgagcc
     1081 gatggagctc gcgcaccctc cgtacaagaa agcgacctac gaggactacg tcggggagcg
     1141 cgcgctcaaa cgcttcggga agaagaagtg gagcaagaaa gtcgcggcgg tggtcgcagc
     1201 gctgcgcgcg gccttcgagc cggacgacgt ggtgctgggc ggcgggaacg tcaagaagct
     1261 cgccgagctg ccgccgggct gccgcgccgg cgacaacgag aacgcctttg cagggggatt
     1321 caaactgtgg gagccgagct ga
//
LOCUS       DASUCJ010001308         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1512052, whole genome shotgun sequence.
ACCESSION   DASUCJ010001308 DASUCJ010000000
VERSION     DASUCJ010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512052"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFU90_15300"
     CDS             complement(<1..575)
                     /locus_tag="VFU90_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481210.1"
                     /translation="MDAIASVAARKLGKFLAKDFREIFGSAQSDEAERLGALARTTIE
                     CIARSDALYHTYEHTMLVTTVGRDILRGRTLAHGIEPEDYVHLIVACLLHDIGFVRGV
                     LSGDTKSEFVVDKSGRTVTLPRGASDASLAAYHVDRSKLFVYERLGKSTTIDAARIAA
                     AIELTRFPPSPNSDYQNGLEPRLVQAADLIG"
     gene            complement(704..>1054)
                     /locus_tag="VFU90_15305"
     CDS             complement(704..>1054)
                     /locus_tag="VFU90_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5481211.1"
                     /translation="TLGTVEYGAVVLGSPLIVVLAHTACGAVKAACDVVTRNATFPGA
                     IGPMIEPIVPAAIAVRDDPGDFADNAAKESARRTAGRLTGASSLIAGLVGAGKVKIAA
                     AIYDLETGAVTYLD"
BASE COUNT          168 a          338 c          362 g          186 t
ORIGIN      
        1 tgtccgatca gatcggcggc ctgaaccagt ctcggttcga gtccgttctg gtagtccgag
       61 ttcggactgg gcgggaaacg ggtgagttcg atcgccgccg cgatgcgggc ggcgtcgatg
      121 gtcgtcgact tcccgagccg ctcgtagacg aacagcttcg accgatcgac atgataggcg
      181 gcgagcgacg catcggacgc gccgcgtggc agcgtgaccg ttcgtccgct cttgtcgacg
      241 acgaactcgg acttggtgtc gccgcttagc acgccgcgga cgaagccgat gtcgtgcagg
      301 agacaggcga cgatgaggtg cacgtaatcc tcgggttcga tcccatgcgc cagcgtccgc
      361 cctcggagga tgtcgcggcc gaccgtggtc acgagcatgg tgtgctcgta ggtgtggtag
      421 agcgcgtcgc tcctggcgat gcactcaatc gtcgttcgcg cgagcgcgcc caggcgttcg
      481 gcttcgtcgc tttgggccga gccgaaaatc tcgcggaagt ctttggccag aaacttgccg
      541 agcttcctgg ccgccaccga ggcaatcgca tccatctcgt cggctcccga agccccccgg
      601 ccagccctgc gcgcccctaa cggtcgtggc cggtcggtag aataatgcag gcgcagcatt
      661 cgcgccagag acagatatgc gggtcttgct gcgacgacgc ctgtcaatcg agatacgtga
      721 cggcgccggt ttcgaggtca tagatcgcgg ccgcaatctt gaccttcccc gccccgacaa
      781 gcccggcgat aagcgagctt gccccggtga gccggccggc ggtccgtctg gcgctctcct
      841 tggcggcgtt gtcggcgaaa tcgccgggat cgtcgcggac cgcgatcgcc gcgggcacga
      901 ttggctcgat catcgggccg atggccccgg ggaacgtggc gttcctggtc accacgtcgc
      961 aggccgcttt caccgcgccg caggcggtat gggcgaggac gacgatgagc ggagagccga
     1021 gcacgacggc gccgtattcg accgtaccga gggt
//
LOCUS       DASUCJ010001309         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1312966, whole genome shotgun sequence.
ACCESSION   DASUCJ010001309 DASUCJ010000000
VERSION     DASUCJ010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312966"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1013
                     /locus_tag="VFU90_15310"
     CDS             <1..>1013
                     /locus_tag="VFU90_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEU5481212.1"
                     /translation="SVPVGSQLLGRVLDGLGRPIDGGPLLVHVPHVDVDHQPPHPLTR
                     RMIEEPLPLGVRALDALVPCGRGQRMGIFAGSGVGKSTLLSMITRGTEAAVTVLALVG
                     ERGREVREFIENDLGPEGLQRSVVVVATSDAPALVRLRAAFTATRIAEHFRDEGKDVL
                     LLMDSLTRFAMAQREVGLSAGEPPATRGYPPSVFALLPKLLERAGASDRGSITGLYTV
                     LVEGDDLMDPIGDASRAILDGHIVLARSLATAGHFPSIDVLESISRVGPAVTSREQRA
                     AATEMRRLLAAYRDAKDLIEIGAYVAGTNPLVDRAVEAKEAMDSFLRQDVHEVTPAQD
                     AWE"
BASE COUNT          159 a          352 c          329 g          173 t
ORIGIN      
        1 cgagcgtgcc ggtcggttcg cagttgctcg gacgcgtgct cgacggtctg ggccggccga
       61 tcgacggcgg cccgttgctc gtgcacgttc cgcacgtcga cgtcgaccat caaccaccgc
      121 atccgttgac ccgccgaatg atcgaagagc cgctccccct cggcgtccgg gcgctcgacg
      181 cgctcgtgcc ctgtggccgc gggcaacgca tgggcatctt cgccggttcc ggcgtcggaa
      241 agtcgacgct gctctcgatg atcacgcgcg gcactgaagc cgccgtcacc gtgctcgcac
      301 tcgtcggcga acgcggacga gaagtgcgcg agttcatcga gaacgacctc ggtccggaag
      361 gactccaacg atctgtcgtc gtcgtcgcga cgtccgacgc gcccgcgctc gttcggttgc
      421 gggctgcgtt caccgcaacg cgcatcgccg agcacttccg cgacgaaggc aaggacgtgc
      481 tcctgctcat ggactcgctc acgcggtttg cgatggcgca acgcgaagtc ggcttgtcgg
      541 caggcgagcc gccggcaacc cgcggctatc cgccgtcggt cttcgcgttg ttgcccaaac
      601 tgctcgagcg cgccggtgcg tcggatcgcg gcagcatcac cggcctctac acggtgttgg
      661 tcgaaggcga cgacctgatg gatcccatcg gcgatgcgtc gcgtgcgatt ctcgacggtc
      721 acatcgtgct cgcgcgctcg ctcgcaactg cggggcactt cccttcgatc gacgtgttgg
      781 aatcgatttc ccgtgtcggc ccggccgtca cgtcacgcga gcaacgcgcg gccgcgaccg
      841 agatgcgacg ccttctcgcc gcataccgcg acgccaagga cttgatcgag atcggtgcgt
      901 acgttgcggg gaccaacccg ctcgtcgacc gcgccgtcga agccaaagaa gcgatggaca
      961 gcttcctccg acaggacgta cacgaggtaa ctcccgcaca ggatgcgtgg gag
//
LOCUS       DASUCJ010001310         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_930992, whole genome shotgun sequence.
ACCESSION   DASUCJ010001310 DASUCJ010000000
VERSION     DASUCJ010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930992"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFU90_15315"
     CDS             <1..607
                     /locus_tag="VFU90_15315"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU5481213.1"
                     /translation="ALARARTAFAAHRKLTVLLRDHRSLAGARAQFDAPSEMCPDLAF
                     ALGPLARTVAPRTRVAWLARQDREAAPHADAGGYATFDWALSRRSPISRAERLGYSFV
                     SAHPSIGPHAHGPLMRLGVAASRQRVKSGCEMLEAGHTVITDRLHGHILSLLLGIPHV
                     VLDNSYGKVRSFYETWTRDAPIVQWAETAADAVRAVEQWSS"
     gene            604..1515
                     /locus_tag="VFU90_15320"
     CDS             604..1515
                     /locus_tag="VFU90_15320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU5481214.1"
                     /translation="MTTLATDAVDMSVIVCTRNRSAALMLLLEHLQRLEVPPGVRWEL
                     VVVDNGSSDDTPALLASLETSLPMRIVREAMPGLSRARNAGLAVARGQVLLFTDDDCV
                     PDPKWLAAVYDEFRRAPTLGVLGGRVELYDARDRPTTTRTSRERRRIAASFDLDGIVG
                     CNMSFRRSAFDAIGPFDVALGGGTGAGAGEDVDFVYRGLRAGIQMEYTPDALVYHNHG
                     RRTDAQVRDLTRAYTVARGALFCKYLLAGDAAILRRIRQDLAFHRRAFVADVRRARFP
                     TSVFRHYAQLGAGAAYWIRHRLFGTIS"
     gene            complement(1559..>3161)
                     /locus_tag="VFU90_15325"
     CDS             complement(1559..>3161)
                     /locus_tag="VFU90_15325"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5481215.1"
                     /translation="HMHRGLVMNVIPEATTMVDGDSVNGVYRYSTTPALGGSMFWDVT
                     NGLGLSATAHPDFSQVEADVAQVTLNQRFALFFPEKRPFFLEGLDQYDTPNRLIYTRD
                     IVQPDAGAKLTGKIGDLNIAYLGAVDQRDSATGSDPVYNILRLRRDLGASSNLGLVYT
                     DRIDGGDYNRMGGVDARFLWRKIWFSQFQFVGSTTRDSLGGRTGALWDITLGDRTGRA
                     YGNHVEFLGVSPNFEAASGFVNRTGFVRAMIFNRYTLYGGPGALVEQVSFFLNAQPLW
                     NYADFGKLGGTFEGGYSAQASTTLRGGWNIGLTVNDNFQRFDSTSYRGYGVVNGADTS
                     AFALPHGLYSLYGKSVSLTSPNRKVTVNATAGIDDAVLFAEAGRGREVTGSLDVAWRP
                     TPLFRFDASWIYDRLTRARDGSEFAITNIPRLQVEYQLTRSIFLRYIGQYVAQRQAAL
                     EDPRTGAPLVRRDSTNGAYATLGAFSSNTFRNDVLLSYKPVPGTVFFAGYGTSLTEPT
                     AFQFSRSMYRTTDGVFVKVSYLFRL"
BASE COUNT          520 a         1008 c         1108 g          525 t
ORIGIN      
        1 cgcgctggcg cgcgcacgga cggcgttcgc ggcgcatcgg aagctcacgg tgctgcttcg
       61 cgatcatcgg agtctcgccg gcgcgcgcgc gcagttcgac gcgccgagcg agatgtgtcc
      121 cgatctcgcg ttcgcgttgg gcccgctcgc acgaacggtt gcgccgcgca cgcgcgtggc
      181 atggctggcg cgccaggatc gcgaggccgc gccgcacgcc gacgcgggcg gctatgcgac
      241 gttcgattgg gcactgagcc ggcgatcgcc gatctcgcga gcggagcgcc tcggctacag
      301 cttcgtgtcg gcgcatccga gcatcggtcc gcacgcgcac gggccgttga tgcggttagg
      361 cgtagctgca tcgcggcagc gggtgaagag cggatgtgag atgctggagg ccggccacac
      421 cgtgatcacg gatcgcctgc acggccacat tctcagcctg ctgctcggca ttccgcacgt
      481 ggtgctcgac aacagctatg ggaaggtgcg ttcgttctac gagacgtgga cgagggatgc
      541 gccgatcgtc cagtgggcag aaaccgcggc cgacgcggtg cgcgcggtgg agcaatggtc
      601 gtcatgacga cgcttgccac cgacgccgtg gacatgagcg tgatcgtctg cacgcgcaat
      661 cgcagcgccg cgctgatgct gctcctggaa catctacagc ggctcgaagt gccccccggc
      721 gtgcgctggg agctggtcgt cgtcgacaat gggtcgtcgg acgacacgcc ggcattgctc
      781 gcctcgctcg agacgtcgct gccgatgcgc atcgtgaggg aagcgatgcc gggtctctcg
      841 cgcgcacgaa acgccgggct cgcggtggcg cgcggccagg ttctgctgtt cacggacgac
      901 gactgcgttc ccgatcccaa gtggctggcg gcagtctacg atgagttcag gcgcgcgccg
      961 acgttaggcg tgctgggcgg ccgggtggag ttgtacgacg ctcgggaccg tccgactacg
     1021 acgcgcacgt cgcgcgagcg ccggcggatc gcggcgtcct tcgacctgga cggcatcgtc
     1081 ggatgcaaca tgtcgttccg gcgatcggcg ttcgacgcga tcgggccgtt cgacgttgcg
     1141 ttaggcggag ggacgggcgc cggcgcgggg gaagacgtgg acttcgtgta ccgcgggctg
     1201 cgcgccggca tccagatgga atacacgccg gacgccctcg tctatcacaa ccatggccgg
     1261 cgcacggatg cgcaggtgcg cgatctgacg cgcgcctaca ccgtcgcccg gggcgctctg
     1321 ttctgcaagt atttgctggc cggcgacgcc gccatcctcc gacgcatccg gcaggatctc
     1381 gccttccacc ggcgcgcctt cgtggccgac gttcgccggg cacggtttcc gaccagcgtg
     1441 ttccgccact acgcgcagct cggcgccggg gcagcgtact ggatccggca ccgcctcttc
     1501 gggacgatct cgtagggcag cgcgagctcg cgaagacgcg gacacaacac gggcacacct
     1561 agaggcggaa caggtagctc accttcacaa acacgccgtc agtcgtgcgg tacatcgagc
     1621 gtgaaaattg gaacgccgtc ggctcggtga gcgacgtgcc gtagcctgcg aaaaagaccg
     1681 tgcccgggac cggcttgtac gagaggagca cgtcgttgcg gaaggtgttc gaggagaaag
     1741 cgccgagcgt cgcgtaggcg ccgttcgtcg agtcgcgccg cacgagcggc gcgccggtgc
     1801 gcgggtcctc gagcgccgct tgccgctgcg ccacgtactg cccgatgtag cgcaggaaaa
     1861 tcgagcgcgt cagctggtac tccacttgca accgcgggat gttcgtgatc gcaaactcgc
     1921 tgccgtcgcg cgcgcgcgtg agacgatcgt agatccacga cgcgtcgaag cggaagagcg
     1981 gcgttgggcg ccaggcgaca tcgagactgc ccgtcacctc gcggccccgg cccgcttcgg
     2041 cgaagagcac cgcatcgtcg atgccggcgg tcgcgttcac ggtgaccttc cggttaggcg
     2101 acgtgaggct cacgctcttg ccgtacaggg aatagagccc gtgcggcagg gcgaacgcgc
     2161 tggtgtcggc gccgttcacg acgccgtagc cgcgatacga cgtgctgtcg aatcgctgaa
     2221 agttgtcgtt gacggtcaga ccgatgttcc agccgccgcg cagcgtcgtc gacgcctgcg
     2281 ccgagtagcc gccctcgaac gtgccgccta acttgccgaa gtcggcgtag ttccagaggg
     2341 gttgggcgtt gagaaaaaac gagacctgtt cgaccagcgc accaggaccg ccatagagcg
     2401 tgtagcggtt gaagatcatg gcgcggacga atcccgtgcg gttcacgaag ccgctcgctg
     2461 cctcgaaatt cggactcacg ccgaggaact cgacgtggtt cccgtaggcc cgtccggtgc
     2521 ggtcgccgag cgtgatgtcc cagagcgcgc cagtacgtcc gccaaggctg tcgcgcgtgg
     2581 tggaccccac gaattggaat tgcgagaacc agatcttgcg ccacaggaac cgcgcgtcga
     2641 cgccgcccat gcgattgtag tcgccgccat cgatgcgatc ggtgtagacg agtccgagat
     2701 tcgagctcgc acccagatcg cgacgcaggc gcagaatgtt gtacacgggg tcgctgccgg
     2761 tggcggaatc gcgctggtcg acggcgccca ggtaggcgat gttcagatca ccgattttgc
     2821 cggtgagttt ggcgccggcg tcgggttgga cgatgtcgcg cgtgtaaatg agccggttgg
     2881 gcgtgtcgta ctgatcgagt ccctcgagaa agaacggacg cttctccgga aagaacaacg
     2941 cgaatcgttg gttgagcgtg acctgcgcca cgtcggcctc gacctgtgag aagtcgggat
     3001 gcgccgtggc gctcaggccc agtccgttcg tcacatccca gaacatcgac ccgccgagcg
     3061 ccggcgtcgt ggaatagcgg tagacgccgt tcaccgagtc gccgtccacc atggtggtgg
     3121 cttccggaat cacgttcatg accaggccgc ggtgcatgtg c
//
LOCUS       DASUCJ010001311         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1328374, whole genome shotgun sequence.
ACCESSION   DASUCJ010001311 DASUCJ010000000
VERSION     DASUCJ010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328374"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..>1051
                     /locus_tag="VFU90_15330"
     CDS             232..>1051
                     /locus_tag="VFU90_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481216.1"
                     /translation="MGCNGSGGGGVAQMQQGSSVKDLMAARKLTEKDVTAALKTYVPP
                     GKHDEYYLFFSGGHSGQVIVAGVPSMRILKVIGVFTPEPWQGYGTGGLGENAVLAGGT
                     QNGRALRWGDAHHPALSETKGDYDGQFLFVNDKANARIAVIDLKDFVTKQIVSDPLIA
                     SNHGATFVTPNTEYVIEGAQYPAPLGRGYADISTFKDTYRGAVTFWKFDRAKGRIDPS
                     QSWAIELPPYSQDLADAGKLDSDGWAFLNSFNTEMATGGDLQGNPTIESGASQND"
BASE COUNT          188 a          342 c          369 g          152 t
ORIGIN      
        1 ggttcggttc ggaccgaagg gcgcgtcgtt gcggccgctg acggcgacca cgctcgaggc
       61 gcaaggcgtg cgccgcgcgg agattcgtgc acgcgcgctc cacgccaatc gcggccaatt
      121 ctcgccgatt cgtgcatcgg ctgcggtgga acggcggttg cgtaacggtc gcggcggagg
      181 aaagccatgc ggtttcgaag cgtcgcagcc tgcgcgtgcc tggtctcggc gatgggttgc
      241 aacgggagcg gcggcggcgg cgtcgcgcaa atgcagcagg gatcgtccgt caaggacctg
      301 atggcggcgc gcaagctcac ggaaaaggac gtgaccgccg cgctcaagac gtacgtgccg
      361 cccggaaaac acgacgagta ctacctgttc ttctctggcg ggcactccgg tcaggtcatc
      421 gtcgccggcg tgccgtcgat gcgcatcctc aaggtcatcg gcgtgttcac gcccgagccg
      481 tggcagggct acggcaccgg cgggctcggc gagaacgcgg tgctggccgg cggcacgcag
      541 aacggccggg cgctgcgctg gggcgatgcc caccacccgg cgctcagcga aacgaagggc
      601 gactacgacg gccagttcct gttcgtgaac gacaaggcca acgcgcgcat cgccgtcatc
      661 gacctgaagg acttcgtcac gaagcagatc gtgagcgatc cgctcatcgc gagcaatcac
      721 ggcgcgacct tcgtcacgcc gaacaccgag tacgtcatcg agggggcgca atacccggcg
      781 ccgctcgggc gcgggtacgc cgacatctcg acgttcaagg acacctaccg tggcgcggtc
      841 acgttctgga agttcgaccg cgccaagggc cggatcgacc cgtcgcagag ctgggccatc
      901 gagctgcccc cgtactcaca ggacctcgcg gatgccggga agctcgacag cgatgggtgg
      961 gcgttcctca actcgttcaa cacggagatg gcgacggggg gcgatcttca gggcaacccg
     1021 accatcgagt cgggcgcgtc gcagaacgac a
//
LOCUS       DASUCJ010001312         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1159866, whole genome shotgun sequence.
ACCESSION   DASUCJ010001312 DASUCJ010000000
VERSION     DASUCJ010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFU90_15335"
     CDS             complement(<1..566)
                     /locus_tag="VFU90_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU5481217.1"
                     /translation="MGTSQENAAALRAFRILEALAESPRPLALADLVDRIGLPKQTVH
                     RILGQLRSSWLVTRSPGGRHYECATRVNRFALNVMLNSGAAAVRQAILRELVDQIGET
                     CNLTMISGTDIVYVDRVETQWSLRVHLQPGSRVPLHCTATGKLLLSLLPRAQRERLIQ
                     SLPLRAATPGTITDRAALRRELAATRKRR"
     gene            739..>1273
                     /locus_tag="VFU90_15340"
     CDS             739..>1273
                     /locus_tag="VFU90_15340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5481218.1"
                     /translation="MRRMFKVRHLILAVMCLMYFITYIDRVNISIAGPILRHEMGLNA
                     TELGLVFSAFAYPYAAMQIIGGWLSDKFGPRRVLAILSVIWAIATILSGLAWGLVSLV
                     FFRFLLGIGEGGAFPSATRAFTYWMPVAERGFAQGITHSFARLGGAATPPIVLAIVAA
                     SGWRESFVILGVLSLAWT"
BASE COUNT          200 a          410 c          432 g          231 t
ORIGIN      
        1 cggcgctttc gcgtcgcggc gagctcgcga cgcagggcgg cgcgatctgt gatcgttccc
       61 ggcgtcgcgg cgcgcagcgg cagcgactga atcagccgtt cgcgctgggc ccttggcaga
      121 agcgagagca gcagcttgcc cgtggcggtg cagtgcaggg ggaccctcga tccgggctgc
      181 aggtgcaccc gcagcgacca ttgggtctcg acgcggtcga cgtacacgat gtcggtcccc
      241 gaaatcatcg tcaggttgca cgtctcgccg atctgatcga cgagctcacg aaggatcgcc
      301 tgtcgcacgg cggccgcgcc ggaattgagc atcacgttga gtgcgaaccg gttgacgcgc
      361 gtcgcgcatt cgtaatgacg cccgccgggg ctgcgggtga cgagccagct cgaccgcaac
      421 tggccgagga tccggtgcac ggtctgcttc ggcaaaccga tgcgatcgac gagatcggca
      481 agcgcgagcg gacgcggcga ttcagcgagc gcttcgagga tccggaaggc ccgcagcgcg
      541 gcggcgtttt cctggctggt ccccatgcat cgattccatc gaaaaacggg acgaaaggtt
      601 catcgaaggc atcacaatgc cacaaaaacg ggacccgagt ccgcgcgcta tcgttcagag
      661 tgaagcgtcg ggtcgatgcc gccgccctgg ggccgaagac ggcggacccg aaccccgatc
      721 gccgttcccg gaggagccat gcgaagaatg ttcaaggtcc gtcacctgat tctcgcggtg
      781 atgtgcctga tgtatttcat tacctacatc gaccgcgtca atatctcgat cgccggcccg
      841 attcttcggc acgagatggg ccttaacgcg accgagctcg gtctcgtgtt ctccgcgttc
      901 gcctacccgt acgccgcgat gcagatcatc ggcggctggc tcagcgacaa attcgggccg
      961 cggagggtcc tcgcgatcct gagcgtcatc tgggcgatcg cgacgattct ttcgggcctc
     1021 gcgtgggggc ttgtgtcgct cgtcttcttc cgcttcctcc tcgggatcgg cgagggtgga
     1081 gcgttccctt cggcgactcg cgccttcacc tactggatgc cggtcgccga gcgcggcttc
     1141 gcgcagggga tcacccacag cttcgcgcgg ctcggcggcg cggcgacgcc gccgatcgtg
     1201 ctggcgatcg tcgccgcgtc gggctggcgc gagtcgttcg tcatcctggg ggtgctgagc
     1261 cttgcatgga cgg
//
LOCUS       DASUCJ010001313         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_472744, whole genome shotgun sequence.
ACCESSION   DASUCJ010001313 DASUCJ010000000
VERSION     DASUCJ010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472744"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VFU90_15345"
     CDS             <1..1461
                     /locus_tag="VFU90_15345"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEU5481219.1"
                     /translation="PAIKLFGESMTEVMFPATKLIRDMSAGEIRAAFVYYIGAGAVAT
                     SGIIALVRALPTIGSAFRAGFDDMRKSRVGQAIAKLRTEDDLPIAATVFGSIGLALFL
                     SFLPQIGVNLLGGILIIVFGFFFTTVSSRICGQIGSSANPISGMTIASLLAISVIFLL
                     LGWTAIDDRVRAISIACVIAVAVANGGNTSQDLKTGFLIGATPRRQQIAILIGGMGSA
                     LAVGWTLTLLNRSYQYEVPEAHAGFTAPATGRSSDGNVVITGDTMSGFHIGGGDSIDT
                     KPYPVARVYVATGTVLPGKYLIDPASHEIRYVVDPGIGGRVREYNGHSLTRLDSPKAT
                     LMALITDGILTHRLPWALVLIGVALAIALEIAGIQSLPVTVGVYLPITTSAGMFAGGV
                     IRWLVERRFKSEDRSIAEIESGPGVLFASGMIAGGAIGGIVIAAIAGKFGSADKLSEI
                     AGLTRAFGHFATDNWPALLLFCGMGIVLYRVGLRRQ"
BASE COUNT          216 a          496 c          494 g          263 t
ORIGIN      
        1 ccggcgatca agctgttcgg cgaatcgatg acggaggtga tgttccccgc gacgaagctg
       61 attcgcgaca tgtcggcggg ggagatccgc gcggcgttcg tctactacat cggcgcgggc
      121 gccgtcgcga cctccggaat catcgcgctg gtgcgcgcgc tgccgacgat cggcagcgcg
      181 ttccgggccg gcttcgacga catgcgcaag agccgcgtcg gccaggcgat cgccaagctg
      241 cgcaccgaag atgacctccc gatcgcggcc accgtcttcg gctcgatcgg actcgcgttg
      301 tttctgtcgt ttctgccgca gatcggcgtg aacctgctcg gcggcatcct catcatcgtc
      361 ttcggctttt tcttcacgac ggtctcgtcg cggatctgcg ggcagatcgg cagctccgcc
      421 aatccgatct cggggatgac gatcgcctcc ctgctcgcga tctcggtcat cttcctgctg
      481 ctcggctgga cggcgatcga cgaccgggtg cgcgcgatct cgatcgcctg cgtcatcgcg
      541 gtggcggtcg cgaacggcgg caacacctcg caggacttga agaccggctt cctgatcggc
      601 gcgacgccgc ggcgccagca gatcgccatt ctcatcggcg ggatgggctc tgcgctggct
      661 gtcgggtgga cactgaccct gctgaaccgg tcgtaccagt acgaggtacc ggaagcccac
      721 gccgggttta cggcgccggc gaccggccgc tcgtccgacg gcaacgtcgt gatcacgggc
      781 gacacgatgt ccggtttcca tatcggcggc ggcgactcga tcgatacgaa gccgtatccc
      841 gtcgcacgcg tgtacgtcgc caccggcacc gtgctccccg gcaagtacct catcgatccc
      901 gcctcgcacg aaatccgcta cgtggtcgac ccgggaatcg gcgggcgggt gcgggagtac
      961 aacgggcaca gcctgacgcg gctcgactct cccaaggcga cgctgatggc gctcatcacc
     1021 gacgggatcc tcacccaccg cctgccctgg gcgctcgtgc tgatcggtgt ggcgctcgcc
     1081 atcgccctcg aaatcgcggg gatccagtcg ttgccggtca ccgtcggtgt gtacctcccc
     1141 atcacgacct cggccggcat gttcgccggc ggcgtgatcc gctggctcgt cgagcgacgc
     1201 ttcaagtcgg aagaccggag catcgccgag atcgaatcgg gaccgggggt cctgttcgcg
     1261 agcggcatga tcgcgggggg cgcgatcggc ggcatcgtga tcgcggcgat cgccgggaag
     1321 ttcggcagcg cggacaagct gtcggagatc gcgggcctga cgcgcgcgtt cggccatttc
     1381 gcgacggaca actggcccgc cctgctgctg ttctgcggca tgggaatcgt cctgtatcgc
     1441 gttgggctgc ggcgccagta gtccaaggg
//
LOCUS       DASUCJ010001314         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1053309, whole genome shotgun sequence.
ACCESSION   DASUCJ010001314 DASUCJ010000000
VERSION     DASUCJ010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053309"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFU90_15350"
     CDS             <1..340
                     /locus_tag="VFU90_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5481220.1"
                     /translation="FALTLDYGKQTMALVANAAFDRRDEYERAGVFLLNRGGKYLVLD
                     VRPGTPAAQAGIVKGDTIETIDGKPASDMSLQALRELFFGQPGTVVHLGLAGKDGTQR
                     NVTLTLQDFV"
     gene            443..1012
                     /locus_tag="VFU90_15355"
     CDS             443..1012
                     /locus_tag="VFU90_15355"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5481221.1"
                     /translation="MKTSFKRAAYAALVAAGTVAFAACSSSAPQEPIATSAPANATAT
                     PAPEQTQGESDAEFVQSAAQTEAGRYALGAIGVQRANAAPLKSLAANMSSDAAASSRW
                     LTAYASKHGIHVDNHPKVRTMYQYSQMTGLTGSAFDRAYAQAVNTDASLSLDAFQTAA
                     HVAADPRLREFAKRAAAQLEANSKQARAF"
     gene            1267..1710
                     /gene="mraZ"
                     /locus_tag="VFU90_15360"
     CDS             1267..1710
                     /gene="mraZ"
                     /locus_tag="VFU90_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU5481222.1"
                     /translation="MHAELPRFTGSVEHALDEKGRLIVPARFRERLGTGFVLTIAEPD
                     PCLALLPTATWANFCARLEGAPRKDEAFRRFVRHLFAHTEEVACDAQGRLVIPATLRA
                     YAGIAREVVSVGSLTRVEIWAKERYAAHAAPQGEVADFMADLGLY"
     gene            1723..>2515
                     /gene="rsmH"
                     /locus_tag="VFU90_15365"
     CDS             1723..>2515
                     /gene="rsmH"
                     /locus_tag="VFU90_15365"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5481223.1"
                     /translation="MTHVPVLLETALEYLAVAPDGIYVDATFGAGGHSRAILEPLTTG
                     RLIALDADPSAEARARRLSDPRLTFVHANFRELSAVLDGCGVGAIDGALFDLGVSSMQ
                     FDDPERGFSIGKAAPLDMRMDPHERRSAYDVLATASERELADIFFQYGEERSARRIAH
                     AIVARRSAGRLPTTTTEFASLVAAVLRRPGRRERLHPATRVFQALRIAVNDELGALRD
                     GLGAAIDRLRGAGRIVAISFHSLEDRRVKEAFRDDPRLTRITKRPL"
BASE COUNT          417 a          827 c          852 g          419 t
ORIGIN      
        1 cttcgcgctg acgctcgatt acggaaagca gacgatggcg ctggtcgcca acgccgcctt
       61 cgaccggcgc gacgaatacg agcgcgccgg cgtgttttta ctcaatcgcg gcggtaagta
      121 cttggtgctg gacgtgcgcc ccgggacgcc ggcggcgcaa gccggcatcg tgaagggcga
      181 cacgatcgag acgatcgacg gcaagcccgc gtccgatatg agcttacagg cgctgcgcga
      241 actcttcttc ggccaaccgg gaacggtggt ccatctcggg ctggccggca aagacggcac
      301 gcagcgcaac gttacgttga cgttgcaaga cttcgtgtaa gcgaaagcac gagcttgcac
      361 ggccggcgag gcggcttgcg tttgcgcgag ccgctttcgc gttatttcga gcgcgcggct
      421 gggtacgttg gaggcgaaag ccatgaaaac gtcattcaaa cgcgccgcgt acgccgcgct
      481 ggtcgccgcc ggaaccgttg cgttcgcggc ctgcagcagc agcgcaccgc aagagccgat
      541 cgcgacgtcg gcgcccgcga acgcgacggc gactccggca cccgagcaaa cccaaggtga
      601 aagcgacgcg gagttcgtgc agtcggccgc gcaaaccgaa gcgggccggt atgcgctcgg
      661 cgcgatcggc gtacagcgcg ctaacgcggc gccgctgaag tcgctcgctg caaacatgtc
      721 gtcggacgcg gccgcgtcga gccgctggtt gaccgcatac gcaagcaaac acgggattca
      781 cgtcgacaac catccgaagg ttcgcacgat gtaccagtat tcgcaaatga cggggctgac
      841 cggcagtgcg ttcgaccgag cctacgctca agcggtgaat accgacgcgt cgctgtcgct
      901 cgatgcgttc caaaccgccg cacacgttgc cgccgatccg cgtttgcgcg agttcgccaa
      961 gcgcgcggca gcgcagctcg aggcgaactc caaacaagcc cgagcgttct aggcatagcc
     1021 gcgcccggca gtccgcgttt gacgcgccac gacccggcgg cacgccgtcg ccgccgcgcc
     1081 ggccgtccgt ttccggcgcc gcccctagcc cgagccttcg gcctttcgaa cgtccggaag
     1141 gccgatttcg cgtgactggc cgatcgtcgc cgtggggctg ctccgcgcgg ccggagcttg
     1201 ccttgggtat cccaggggcg tatagtggag caaagtggtg tctggtggtg cctcagggag
     1261 ggctgtgtgc acgcggagct accgcgcttt accgggtcgg tggagcatgc tcttgatgaa
     1321 aagggcaggc ttatcgtccc cgcgcgcttc cgggagcgtc tcgggacggg cttcgtgctc
     1381 acgatcgccg agccggatcc atgcttggca ctgcttccca cggctacctg ggcgaacttt
     1441 tgtgcccggc tcgagggcgc accccgtaaa gatgaggcct tcagaaggtt cgtccgccac
     1501 ctctttgccc atacggagga ggtcgcgtgc gatgcccaag gccgcttggt catccccgcc
     1561 acgttgcgtg cctacgccgg catcgcacgc gaggtcgtct cggtcggctc gctgacgcga
     1621 gtcgagatct gggccaagga acgctacgcc gcccatgccg cgccgcaggg agaggtcgcc
     1681 gatttcatgg ccgacctggg gctgtactga tgtggagcgc gcatgacgca cgtcccggtg
     1741 ctgttggaaa ccgccttgga atacttggcg gtcgcgccgg atgggatcta cgtggatgcc
     1801 acctttggag cgggcggcca ttcgcgagcc atcttggagc cgctcaccac gggacggctg
     1861 atcgcgttgg acgccgatcc ttcggcggaa gcacgcgcgc ggcgcctgag cgatccgcgg
     1921 ctgaccttcg tccacgcgaa ctttcgggag ctgagcgcgg tgctcgatgg ctgcggggtg
     1981 ggggcgatcg atggagcgct cttcgatttg ggagtctcgt cgatgcagtt tgacgatccg
     2041 gaacgcggct tttcaattgg aaaggcggcg ccgctcgaca tgcggatgga cccgcacgaa
     2101 cgccgcagcg cctacgacgt tctcgccacc gcgagcgagc gcgaactggc cgacatcttc
     2161 tttcagtacg gtgaggaacg atcggcgcgc cggatcgccc acgcgatcgt cgcacgccgg
     2221 tcggccggcc ggctgccgac gacgaccacc gagttcgcct cgctggtcgc cgcggtgctg
     2281 cgccggccgg ggcgccgcga acggctccat cccgctaccc gcgtcttcca agcgctacgg
     2341 atcgccgtca acgacgagct gggcgcgctg cgcgacggac tgggcgcggc catcgatcgg
     2401 ctgcgcggcg ccgggcgcat cgtcgccatc agttttcatt cgctcgaaga ccggcgtgtg
     2461 aaggaagcgt ttcgcgacga tccacggttg acacgaatca ccaaacgacc gctac
//
LOCUS       DASUCJ010001315         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_426408, whole genome shotgun sequence.
ACCESSION   DASUCJ010001315 DASUCJ010000000
VERSION     DASUCJ010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426408"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFU90_15370"
     CDS             complement(<1..333)
                     /locus_tag="VFU90_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5481224.1"
                     /translation="MPPTVFILDFGAQYSQLIARRTRELGVYSEIVPFDTPWETLAAR
                     DPAAIVLTGGPESTLVPGAPGVDPALLHSGVPILGICYGMQLLARDLGAELIKLERAE
                     YGPATLWVV"
     gene            complement(349..891)
                     /gene="tpx"
                     /locus_tag="VFU90_15375"
     CDS             complement(349..891)
                     /gene="tpx"
                     /locus_tag="VFU90_15375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU5481225.1"
                     /translation="MTIHNVEERAGAVTFKGKPMTLQGPALREGDPAPEFNLTARDLS
                     NVTVHELIDGGARAAMLIVVPSLDTSVCSLESHKFNQRLGELPAGVTASVVSMDLPFA
                     QARWAGAQEGEVKLEMLSDYRDHSFGRNYGLLIGELGLLARAIVVIGKDKSVKYLQIV
                     GDVADEPDYDAALKAAAAAA"
     gene            complement(929..>1557)
                     /locus_tag="VFU90_15380"
     CDS             complement(929..>1557)
                     /locus_tag="VFU90_15380"
                     /inference="COORDINATES: protein motif:HMM:NF018656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1297 domain-containing protein"
                     /protein_id="HEU5481226.1"
                     /translation="APHAKVSFERAFFLASSPQEYAATSGGLLRAGMITEEGLAGAVI
                     EEYALGPSVNLNFFYSPILRELELLGTDTRRQTNLEGFRNVPPAALHALRGIPMRLEE
                     AGHIAATLTESMLEKAFEMGERFVAAAEAANPPGTIGPFALQCTIVAGPPKEFVCYDV
                     SLRIPGSPGTRYTPYSAYRWGRDVSVGERIAMEVVWARDTNRLPDVLT"
BASE COUNT          276 a          541 c          474 g          266 t
ORIGIN      
        1 gaccacccaa agggtcgcgg ggccgtactc ggcgcgctcg agtttgatca gctccgcacc
       61 caagtcgcgg gcgagcagct gcatgccgta gcagatgccg agaatcggaa cgcccgagtg
      121 caggagcgcc ggatcgacgc ccggcgcgcc gggcacgagc gtgctctccg gccctccggt
      181 cagcacgatc gccgcaggat cgcgcgccgc cagcgtctcc cacggcgtat cgaacggaac
      241 gatctcggag tataccccca gttcgcgcgt ccgccgcgcg atcagctggc tatactgggc
      301 tccgaagtcg aggatgaaaa ccgtcggcgg catgcaggga cgaacgcgct acgccgccgc
      361 cgccgctgcc ttcagcgccg cgtcgtagtc gggctcgtcg gcaacgtcgc cgacgatctg
      421 taggtacttc acgctcttgt ccttaccgat gacgacgatc gcgcgcgcca gtagacctag
      481 ctcgccgatc agcagcccgt aattccgccc gaagctgtga tcgcgatagt cggacagcat
      541 ctcgagcttc acttcgcctt cttgcgcgcc cgcccagcgg gcttgggcga acggcaaatc
      601 catgctcacg accgatgcgg tgactccggc cggcagctcg ccgagccgct gattgaattt
      661 atgcgactcc agcgagcaca cgctggtgtc gagcgacggc acgacgatca gcatcgccgc
      721 gcgcgcgcca ccgtcgatca gctcgtgcac cgtaacgttc gaaaggtcgc gggccgtcaa
      781 gttgaactcg ggcgccggat cgccctcgcg caaagcgggt ccctgcagcg tcatcggctt
      841 acctttgaag gtgaccgctc ccgcccgctc ctctacgtta tggatcgtca tatcgtgcct
      901 ccctgtttta aaatgacggt cgaataaact atgttaaaac gtccggcagc cgattggtgt
      961 cgcgcgccca gacgacttcc atcgcaatcc gctccccgac cgagacgtcg cgcccccagc
     1021 ggtaggccga atacggcgtg taccgcgtac ccggcgaccc cggaattcgc agcgaaacgt
     1081 cgtagcacac gaactctttc ggggggcctg cgacgatcgt acactgcagt gcgaacgggc
     1141 cgatcgttcc cggcggattg gccgcctcgg ccgccgctac gaatcgctcc cccatctcaa
     1201 acgccttttc gagcatcgat tcggtcagcg tggctgcgat gtgcccggcc tcctcgagcc
     1261 gcatcgggat gccgcgcagc gcgtggagcg ccgccggcgg aacgttgcga aagccttcca
     1321 ggttcgtctg ccggcgcgta tcggttccga gcagctccag ctcccgcaga atcggcgaat
     1381 agaagaaatt caagttgacc gacggtccga gtgcgtattc ctcgatcacc gcgccggcca
     1441 ggccttcttc ggtaatcatg ccggcccgca gcagaccgcc cgaggtcgcc gcgtattcct
     1501 gcggcgacga cgccaggaaa aacgcgcgct cgaaactgac tttggcgtgc ggcgctt
//
LOCUS       DASUCJ010001316         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_487723, whole genome shotgun sequence.
ACCESSION   DASUCJ010001316 DASUCJ010000000
VERSION     DASUCJ010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487723"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1280
                     /locus_tag="VFU90_15385"
     CDS             252..1280
                     /locus_tag="VFU90_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5481227.1"
                     /translation="MREHGLRELLRLGANESPFGPSPRAVAAMSAELDRLGWYGDPES
                     LDLRDALAAKHRCAPEQIVVGSGIDDVMGLAVRAFVAPDATVVTTRGTYPTLNYHAAG
                     YGGRLEYGRYRDDGTVDLEALVTAAQRSGASLAYLANPDNPSGTFLPRDEIARFCEAL
                     PSGTLLLLDEAYADFVDDAALLPPVFEDRIVRMRTFSKAYGMAGARIGYALTSAANVR
                     TFQKIRLHYGINRNAQVGALASLGDDAFRDWVVAETAKARAGYYALARELGCGYIESH
                     ANFVCVDMGTAQRATRVMSALLERAVWIRKPGAPPLDRYVRVSAGTPPQREAFAAALR
                     RVVAELAA"
     gene            1277..>1707
                     /locus_tag="VFU90_15390"
     CDS             1277..>1707
                     /locus_tag="VFU90_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5481228.1"
                     /translation="MRYAVISDVHGNLESLERALALLAPDDALICLGDMVGYGPNPNE
                     CVRLLRDRTTHAVLGNHDLAALENFGTEYFNDAARAAIEWTQRNLDAESRTWLNGLSY
                     ELRLPDFLLVHGAPVNYFEYILDKRAAARAFEQTDARLIFIG"
BASE COUNT          266 a          565 c          589 g          287 t
ORIGIN      
        1 gtacgttccg gtcgcgctgc ggaatggcga cggagcgccg gcggcgcatc ccctaccgct
       61 gcattcgttt tcggtgagtc tgacggcgcg cgccgacgga tacgtggtgg tcggcgagcg
      121 ggaagctccg ctgcccgcgg gggcgccggt cgtcgttcac cgctttctcg gaggatagat
      181 tcctggttcg tcccacgccc gcgatcgaag cgatccccgc aacgaccccg ttcgtcggcc
      241 ccgagcaact catgcgcgag cacgggctgc gcgagctgct gcggctgggt gcgaacgaaa
      301 gcccgttcgg gccgtcgccg cgcgcggtcg cggcgatgag cgcggagctc gatcgcctcg
      361 gctggtacgg cgatccggaa tcgctggatt tgcgcgacgc tttggcggcg aaacatcgct
      421 gcgcaccgga gcagatcgtc gttgggtcgg ggatcgacga cgtgatgggg ctcgcggtgc
      481 gcgcgttcgt ggcgccggat gcgacggtcg tcacgacccg gggcacctat ccgacgctga
      541 actatcacgc tgccggctac ggcggacggc tcgaatacgg ccggtaccgc gacgacggca
      601 ccgtcgatct cgaggcactc gtgacggccg cgcaacgttc cggcgcgtcg ctggcgtatc
      661 tcgccaatcc ggacaatccg agcggtacgt ttttgccgcg cgacgagatc gcgcgcttct
      721 gcgaagcgct gccgtccggc acgctcctgc tgctcgacga ggcctacgcc gatttcgtcg
      781 acgacgcggc gctgctcccg cccgtcttcg aagaccggat cgtccggatg cgaacgttct
      841 cgaaggcgta cgggatggcg ggggcgcgca tcggctacgc gctgacgtcg gcggccaacg
      901 tgcgtacctt tcaaaaaatc cggctgcact acggaatcaa tcgcaacgcg caggtggggg
      961 cgctcgcgtc gctgggcgac gacgccttcc gcgattgggt ggtcgcggaa accgcgaagg
     1021 cgcgcgccgg ctactacgcg ctggcccgcg agctggggtg cggatacatc gagtcgcatg
     1081 cgaacttcgt gtgcgtcgac atgggaaccg cgcaacgcgc gactcgagtg atgagcgcac
     1141 tgctcgaacg cgccgtgtgg attcgcaagc cgggcgcgcc gccgctggac cggtacgtgc
     1201 gcgtgagcgc cgggacgccg ccgcagcgcg aagcatttgc cgcggcgctg cgccgggtgg
     1261 tggccgaatt agccgcatga gatacgccgt tatctccgac gtccacggga atctcgaatc
     1321 gctggaacgc gcgctcgcgc tgctcgcacc cgacgacgcg ctgatctgtt tgggcgacat
     1381 ggtcggctac ggcccgaatc ccaacgaatg cgtgcggctg ctgcgcgacc gtacgaccca
     1441 cgccgtgctc ggcaatcacg atctggcggc gctggagaac ttcggcaccg agtactttaa
     1501 cgacgccgcc cgcgccgcca tcgagtggac gcaacgaaat ctcgacgcgg aaagccgcac
     1561 ctggctcaac ggcttatcgt acgagcttcg gctgcccgat ttcttgctgg ttcacggcgc
     1621 gccggtgaac tacttcgaat acattttgga caagcgggcc gccgcgcgcg ctttcgagca
     1681 aaccgacgcg cgtttgatct tcatcgg
//
LOCUS       DASUCJ010001317         1277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_258245, whole genome shotgun sequence.
ACCESSION   DASUCJ010001317 DASUCJ010000000
VERSION     DASUCJ010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258245"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFU90_15395"
     CDS             <1..314
                     /locus_tag="VFU90_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5481229.1"
                     /translation="PVAAFIRFRDEAQAIALANDSEYGLSASVWTQNIGRANRVAAAL
                     RSGTVAINTPYAIFPGVPFGGYKQSGYGRELGFETMALYSETKSVLTYIGEKPIDPFG
                     V"
     gene            295..642
                     /locus_tag="VFU90_15400"
     CDS             295..642
                     /locus_tag="VFU90_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481230.1"
                     /translation="MIHLEYDVIEPLKIGQRYQTTYGTLWKDGTYFARGTRVILVGLH
                     LEDGFCVRFPTDVGGTPLETWEDWSESDFLAADGSIEETAPLSPAMLAEAREVLADAE
                     DGCHLHLHEGEET"
     gene            755..>1277
                     /locus_tag="VFU90_15405"
     CDS             755..>1277
                     /locus_tag="VFU90_15405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5481231.1"
                     /translation="MNLRLLFFSVMAAVLLVPSYAGAAENTGTISGSIHDVSGAAIAN
                     ATIIASGPATRQSSSDAAGNFSLSVPAGIYRVDITKGGYLAATETDVTVLAGASVPLT
                     VTLQQADLSSLKTIARVTTSARSSINTGAAAIAIAGRQEFANLASPQINDVVERIPGA
                     DVEKGSSSPNTSFT"
BASE COUNT          255 a          391 c          393 g          238 t
ORIGIN      
        1 gacccgtcgc ggcgttcatc cgcttccggg atgaagcgca agccatcgca cttgcgaacg
       61 attcggagta cggcctttcg gccagcgtgt ggacgcagaa catcggccgg gccaaccgcg
      121 tcgcggcggc gctgcgcagc ggaaccgttg ccatcaatac gccgtatgcg attttcccgg
      181 gcgtgccgtt cggcggatac aagcaatcgg gatacggccg agaattgggt ttcgaaacga
      241 tggcgctcta ctccgagacc aagagcgtgc tcacgtatat cggggaaaaa ccgattgatc
      301 catttggagt atgacgttat cgaaccgctg aagatcgggc agcgctacca gacgacgtac
      361 ggcacgctgt ggaaagacgg cacgtacttc gcccgcggga cgcgggtcat actcgtaggc
      421 ctccacctcg aagacgggtt ctgtgttcgc ttcccgacgg acgtcggcgg gacgccgctc
      481 gagacgtggg aggactggag cgagtcggac tttctcgccg ccgacggcag catagaagag
      541 accgccccgc tctcgccggc gatgctggcc gaagcacgag aggtgcttgc cgatgccgag
      601 gacggctgcc atctgcactt gcacgagggc gaagagactt aagtctctta tggatctaaa
      661 atatccacaa ggtgcgcgcc ggctcgcgct ccaagtcaag caccggccac gattctcgag
      721 gccggttctt ttttgttttt gaaaaggatt tttcatgaac ctacgccttt tatttttctc
      781 ggtgatggcg gccgtcttac tcgtgccgtc atatgcgggc gccgctgaaa acacgggcac
      841 gatttccgga agcatccacg acgtttccgg cgctgcgatt gcaaacgcga cgatcatcgc
      901 gagcggtccg gcgacgcggc agtcctcgag cgacgcagcc ggcaactttt cactgagcgt
      961 tccggcgggc atctatcgcg tcgacatcac caaaggcggc tatctcgctg ccacggagac
     1021 cgacgtgacg gtgctggcgg gtgcgagcgt tccgcttacg gtaacgctgc agcaagccga
     1081 cctctcatca ctcaaaacga tcgcgcgcgt gacgacctcg gcgcgcagct ccatcaatac
     1141 gggagccgct gcgatcgcga tcgccggacg ccaagagttc gcgaatcttg cttccccgca
     1201 gatcaacgac gtcgtcgaac gcattccggg cgcggacgtc gagaaaggct cgagctcgcc
     1261 gaacacgagc ttcacgg
//
LOCUS       DASUCJ010001318         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_808853, whole genome shotgun sequence.
ACCESSION   DASUCJ010001318 DASUCJ010000000
VERSION     DASUCJ010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808853"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFU90_15410"
     CDS             <1..534
                     /locus_tag="VFU90_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5481232.1"
                     /translation="DAAGAQQFHFVGCSLGGVVGFELWKRAPERLRSLTFVGSFAWYP
                     DAAQYVRSVIASVEAAGTMERFAFERAQRLGLPAGTRTAETIAQMACKTVPSYVAATH
                     ATWTGEYRELLAKITVPSLVICGGRDAVAPPSFSQEIAAGIRGARLEVIEEAGHVTNA
                     DAPDRFNDLLRAFLKSP"
     gene            complement(497..>1030)
                     /gene="fdhD"
                     /locus_tag="VFU90_15415"
     CDS             complement(497..>1030)
                     /gene="fdhD"
                     /locus_tag="VFU90_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU5481233.1"
                     /translation="LERHFTMSSACGVCGKANIEALQTRAAPIDDSVRIPRSLIAELP
                     DKMRQAQRVFDSTGGLHAAALFDANGALLVLREDVGRHNALDKIAGWALLNDRLPLRG
                     CILLVSGRASYELVQKSIVAGIPVLCAVSAPSSLAVDLARNFNMTLLGFVRGSRSNVY
                     AGRERITEISKTREADR"
BASE COUNT          167 a          309 c          358 g          196 t
ORIGIN      
        1 gatgcggcgg gcgcgcagca gttccacttc gtcggctgca gtttgggcgg cgtcgtcggt
       61 ttcgagcttt ggaagcgcgc gccggagcgg ctgcgttcgc tcacgttcgt cggcagtttt
      121 gcgtggtatc cggatgccgc gcaatatgta cgatcggtga tcgcgagcgt cgaggccgcg
      181 ggaacgatgg agcgtttcgc gttcgagcgg gcgcagcggc tgggcttgcc ggccggaacg
      241 cgaaccgcgg agacgatcgc gcagatggcg tgcaagaccg taccgtcgta cgttgccgcc
      301 acgcatgcga cgtggacggg tgaatatcgc gagttgcttg cgaaaataac cgtgccgtcc
      361 ttggttatct gcggcggccg cgatgccgtc gcgccgccct cgttttcaca ggagattgcg
      421 gccggcatcc gcggcgcgcg tctggaggtt atcgaagagg cggggcacgt caccaacgcc
      481 gacgcgcccg atcgttttaa cgatctgctt cgcgcgtttt tgaaatctcc gtaatgcgtt
      541 cgcggcccgc gtagacgttc gaacgcgatc cgcgcacgaa gccgagcagc gtcatgttga
      601 agttgcgcgc gaggtcgacg gcgaggctgc tgggtgcaga taccgcgcac aagacgggaa
      661 tgccggcgac gatgcttttc tgcacgagtt cgtaactggc gcgtccgctg accagcagaa
      721 tgcagccgcg cagcggaaga cggtcgttca gcagcgccca gccggcgatc ttgtcgagcg
      781 cgttgtgacg gccgacgtcc tcacgcagca cgagcagcgc tccgtttgcg tcaaaaagcg
      841 cggctgcgtg cagaccgccc gtcgagtcga agacccgctg tgcttgccgc atcttatcgg
      901 gcagctccgc gatcaggctg cgcggaatgc ggacggagtc gtcgataggt gcggcgcgcg
      961 tttgcagcgc ttcgatgttc gcctttccgc agacgccgca tgcgctgctc atcgtaaaat
     1021 gccgttcgag
//
LOCUS       DASUCJ010001319         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1420132, whole genome shotgun sequence.
ACCESSION   DASUCJ010001319 DASUCJ010000000
VERSION     DASUCJ010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420132"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFU90_15420"
     CDS             <1..257
                     /locus_tag="VFU90_15420"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU5481234.1"
                     /translation="TPPHGRASGSALKLLVRPERIEIAREAAGENVFPARIVRDRFFG
                     ATRQVEIAAGSGRLEIETALRDHVERVRVPREAIQFLNFR"
     gene            273..>1110
                     /locus_tag="VFU90_15425"
     CDS             273..>1110
                     /locus_tag="VFU90_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5481235.1"
                     /translation="MKSPIRLIACCAAALAAALAAALAPAQTVLDSPELYSGEKALYE
                     AAKKEGMVVSFDTGPTWANWAAEFAAFKKRYPEVEIVYNDIGSAATVVALDKARNRPQ
                     ADTAYYFAGSAFDAVAKNVVAPFKPVNFDKLTPVFRDPDGKWFTIHTLTVAFIVNTKL
                     VKNVPQSWADLLKPEYKNTIVYLDPRSTGVGQVVAFAANFAAGGDMSNIQPGMDYFAK
                     LHRSGNVLRVLGTTPYAQFVKGEIPIWISYENDGLKAKYTDGLGDAVAVVIPKEASAA
                     APY"
BASE COUNT          200 a          368 c          360 g          182 t
ORIGIN      
        1 ccacaccgcc gcacgggcgc gcttccgggt cggcgctcaa gcttctggtg cgtcccgagc
       61 gcatcgagat cgcgcgcgag gctgcgggcg agaacgtgtt tccggcgcga atcgtgcgcg
      121 accgcttctt cggcgcgacc cggcaggtcg agatcgccgc cgggtccgga cgactcgaaa
      181 tcgagaccgc gttgcgcgat catgtcgagc gcgtgcgcgt cccgcgcgaa gcgatccagt
      241 tcctgaactt tcgttagcca gaggaggaag ccatgaagag tcccattcgg ctcatcgctt
      301 gctgtgccgc cgcactcgcc gccgcgcttg ccgctgcgtt ggctccggcg cagaccgtcc
      361 tcgattcgcc cgagctctac tccggagaga aggcgctcta cgaggcggcg aagaaggagg
      421 gcatggtggt gagcttcgac acgggtccga cctgggcgaa ctgggcggcc gaattcgccg
      481 ccttcaagaa gcgctatccc gaagtcgaga tcgtctacaa cgacattggc tcggcggcga
      541 cggtcgttgc gctggacaag gcgcgcaacc ggccgcaggc cgacaccgcc tactacttcg
      601 cgggctcggc tttcgacgcc gtggcgaaga acgtggtggc gccgttcaag ccggtgaact
      661 tcgacaagct cactcccgtg ttccgcgatc ccgacggcaa gtggttcacg atccacacgc
      721 tcaccgtggc gttcatcgtc aacaccaagc tcgtgaagaa cgtgccgcag tcgtgggccg
      781 atctgctgaa gcccgaatac aagaacacca tcgtgtatct cgacccgcgg tcgaccggcg
      841 tcgggcaggt ggtcgccttc gccgccaact tcgccgccgg cggcgacatg agcaacatcc
      901 agccgggcat ggattacttc gcgaagctcc accggtcggg caacgtgctg cgcgtcctgg
      961 gcacgacgcc gtacgcgcag ttcgtgaagg gcgagatccc gatctggatc tcctatgaga
     1021 acgacggact caaggcgaaa tacaccgacg gactcggcga cgcggtcgca gtcgtgatcc
     1081 cgaaggaagc gtcggccgca gccccatatg
//
LOCUS       DASUCJ010001320         1978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_640658, whole genome shotgun sequence.
ACCESSION   DASUCJ010001320 DASUCJ010000000
VERSION     DASUCJ010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640658"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1089
                     /gene="gap"
                     /locus_tag="VFU90_15430"
     CDS             76..1089
                     /gene="gap"
                     /locus_tag="VFU90_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5481236.1"
                     /translation="MAIRVGINGFGRIGRQVVRAAAQQGLTDLEFVAINDLTDTKTLA
                     HLFKYDSVHRTYPGVVTHGDGCIHIDGREIKVLVEKDPAALPWKQLGVSLVLESTGKF
                     TKAEDAKKHLAAGARKVIISAPAKNEDITIVMGVNHETYDAQRHHIISNASCTTNCLV
                     PMVRVLLDNFGMRHASMTTIHSYTNDQNVLDLPHKDLRRARAAAVSMIPTTTGAAKAT
                     SLVIPEVKGKIDGLAVRVPTADVSFTDLVAEVERPTTIADVNAAFRKASESGPLKGYL
                     AYSDEELVSADYIGNPYSCILDAKTTNVIDGTLVKVTGWYDNEWGYASRCVDLLRYVG
                     ERL"
     gene            1086..1358
                     /locus_tag="VFU90_15435"
     CDS             1086..1358
                     /locus_tag="VFU90_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481237.1"
                     /translation="MSPHVEPGARRRDLIALLILAAGAAVAVYGYLGLHTMASKPIVR
                     EPGHTAIQRAISYNNVVVAGWVIIAVGILAVVWSFVANRRSSHASR"
     gene            1358..>1978
                     /locus_tag="VFU90_15440"
     CDS             1358..>1978
                     /locus_tag="VFU90_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5481238.1"
                     /translation="MAKRTINDLTDAEVRDKRALVRVDFNVPMDDRGAVTDDTRMRAA
                     LPTFDALFARGARTIVLLSHLGRPKGGPDPRYSLAPVARHLATITTRPVRFVTSTVGP
                     EAVQATTTAARGTTLLMENTRFLTGEEKNDDALARQMAQLGDLYVDDAFGSAHRAHAS
                     TEGVAHHLHPAVAGLLMDTELRFLGDALAHPQRPFVAILGGAKISGK"
BASE COUNT          368 a          677 c          622 g          311 t
ORIGIN      
        1 atcatcagca tcgtgacctt tggccgagcg ccggcaatcg gcgatgccgg ctgatcaatc
       61 ggagcagacc acgacatggc aattcgcgta gggatcaacg gcttcggccg catcggacgg
      121 caagtggtcc gcgcggcagc gcagcaaggc ctcaccgacc tcgagttcgt cgccatcaac
      181 gatctcaccg acacgaaaac gctcgcgcat ctgttcaagt acgactccgt ccatcgcacg
      241 tacccgggcg tggtgacgca cggcgacggg tgcatacaca tcgacggccg cgagatcaag
      301 gtgctcgtcg agaaagatcc agccgcgctg ccgtggaagc agctcggcgt gtcgctcgtg
      361 ctcgagtcca ccggcaagtt caccaaggcc gaggacgcga agaagcacct ggcggcaggc
      421 gcgcggaagg tgatcatttc ggcgccggcc aagaacgaag acatcaccat cgtgatgggc
      481 gtgaaccacg aaacgtacga tgcccaacgg caccacatca tctcgaacgc gtcctgcacc
      541 acgaactgtc tggtgcccat ggttcgcgtg ctgctcgaca acttcgggat gcgtcacgcg
      601 tcgatgacca cgatccacag ctacacgaac gaccagaacg tgctcgacct gccgcacaaa
      661 gatctgcggc gcgctcgtgc ggcggccgtc tcgatgattc cgacgacgac cggcgccgcg
      721 aaggcgacgt cgctcgtgat tcccgaagtg aaaggcaaga tcgacggcct cgccgtacgc
      781 gtgcccaccg ccgacgtgtc gttcaccgat ctcgtcgcgg aggtcgagcg cccaacgacg
      841 atcgccgacg tcaacgcggc cttccgtaag gcatcggaat cgggacccct caagggctat
      901 ctcgcgtaca gcgacgaaga actcgtctcg gccgactaca tcgggaatcc gtactcctgc
      961 attctcgacg cgaagaccac gaacgtcatc gatggcacgc tcgtgaaggt gacgggctgg
     1021 tacgacaacg agtggggcta cgcgtcgcgc tgcgtcgatc tgctccggta cgtcggcgag
     1081 cggctgtgag cccgcacgtc gaaccggggg ctcgtcgccg cgatctcatc gcgctcctca
     1141 tcctcgctgc cggcgcggcc gtcgcggtct acggctatct cggattgcac accatggcgt
     1201 ccaagccgat cgtgcgcgaa ccagggcaca cggccatcca gcgcgccatc tcgtacaaca
     1261 acgtggtcgt cgccggctgg gtgatcatcg ccgtcgggat tctcgcagtg gtctggtcgt
     1321 tcgtggcaaa ccggcggtcg tcgcacgcgt cgcgctgatg gccaagcgga cgatcaacga
     1381 cctcacggac gccgaggtgc gcgacaagcg agcactcgtg cgggtcgatt tcaacgtgcc
     1441 gatggacgac cgcggcgcgg tcaccgacga cacgcgcatg cgcgccgcgc tcccgacttt
     1501 cgatgcgctc tttgcgcgcg gtgcgcgcac catcgtgctc ctctcgcacc tggggcgtcc
     1561 caagggcgga cccgatccgc gctactcgtt ggcgcccgtc gcgcggcatc tcgccacgat
     1621 caccacgcgt cccgtgcgat tcgtgacaag caccgttggt cccgaagcgg tgcaggcaac
     1681 gacgaccgcc gcgcggggca ccacgctcct catggagaac acgcgcttcc taacgggcga
     1741 ggagaagaac gacgacgccc tcgcgcgcca gatggcccaa ctgggcgatc tctacgtgga
     1801 cgacgcattc ggctcggcgc atcgcgcgca cgcatccacc gagggcgtgg cgcaccatct
     1861 gcatccggcg gtggccgggt tgctcatgga caccgagctc cgttttctgg gcgacgcgct
     1921 cgcacatccg cagcggccgt tcgtcgcgat cctgggcggc gcaaagatct cgggcaag
//
LOCUS       DASUCJ010001321         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_502598, whole genome shotgun sequence.
ACCESSION   DASUCJ010001321 DASUCJ010000000
VERSION     DASUCJ010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502598"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VFU90_15445"
     CDS             complement(<1..1659)
                     /locus_tag="VFU90_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481239.1"
                     /translation="MLLENVGATIAGVPIRLGGGVYGFVAPSFDLTVAGNGDLRRLRA
                     VLNQSHGVPLAGPARLGVRVEGPVSKPLTLIALSAPHASYASAPFDATSVLAAFDGQQ
                     FDLVDAHATYAGIALGARGRLNLRSQPRALEMVATADAPAGGLPYASAALGGMPLHAT
                     VVATGDRPGAAILRGVVTGANRIGNLTGTFALAGTGAGTIGPLQITGPNESLYAIAAV
                     DGRRNLDGYVDARNLLVDDRQTPPLPGVNLPQLPAMLGRVDANMRARIRNGTIQATGT
                     VQVRNGRIDGTAIARANLRFGTTRAHPLVAVLNAAGVGPLRADAAATIAYAGNTVIVR
                     DAAAAAPGTFVDARGSITGVARSVPHYDFDAELHSADVASLAELLRAPPRVPLEGELE
                     ARVHVGGSGATPIVDGSIAVPAGAVNGLAFHDLRTGISGSAAGIALHGGTVGVGSSEI
                     AFAGAANATTQRLSVAAPHVDLEDFDDYFDAGDVLRGNGSVRAAVTVANGRIVASSGS
                     AQLQSAAFRSFDLGAARARWNGSADRIATQLSFGGPSGIVAAAGS"
BASE COUNT          258 a          595 c          576 g          240 t
ORIGIN      
        1 gcttccggcc gccgccacaa tgcccgacgg tccgccgaac gagagttgcg tcgcgatgcg
       61 atccgcgctg ccgttccagc gcgcgcgagc cgcgccgagg tcgaagctgc gaaatgcggc
      121 cgactgcagt tgcgcgctgc cgctgctcgc aacgattcgg ccgttcgcga cggtcaccgc
      181 cgcacgcacg ctgccgttcc ctcgcagcac gtcgccggcg tcgaagtaat cgtcaaaatc
      241 ctccagatcg acgtgcggcg ccgcgacgct caggcgttgc gtcgtcgcgt tcgccgcccc
      301 ggcgaacgcg atctcggagg agccgacgcc gaccgtaccg ccgtgcaagg cgatgccggc
      361 cgccgatccg gatattccgg tccggagatc gtggaatgcg agcccgttga ccgcgcccgc
      421 gggcaccgcg atgctcccgt cgacgatcgg agtagcgccg gaaccgccga cgtgcacgcg
      481 cgcctccagt tcgccctcca acggaacgcg cggcggggcg cgcagcagtt cggcgagcga
      541 cgcaacgtcg gcggaatgca gctcggcgtc gaaatcgtag tgcggtacgc tgcgcgcgac
      601 cccggtgatg ctgccgcggg cgtcgacgaa cgttcccggc gcggcggccg ctgcatcgcg
      661 cacgatgacg gtgttgccgg cgtaggcgat cgtcgcggcg gcgtcggcgc gcaacgggcc
      721 gaccccggcg gcgttcaaca ccgcgacgag cgggtgcgcg cgcgtggtcc cgaacctcag
      781 gttcgcgcgc gcgatcgcgg taccgtcgat gcgcccattg cgaacctgga ccgtacccgt
      841 cgcttggatc gtgccgttgc ggatgcgggc acgcatgttt gcgtctactc ggcccaacat
      901 cgccggcaat tggggaaggt tcacgccggg aagcgggggc gtttgccgat cgtcgaccag
      961 taggttgcgc gcgtcgacgt agccgtcgag gttgcggcgg ccgtcgacgg cggcgatagc
     1021 gtacaacgac tcgttaggtc ccgtaatttg cagcggtccg atcgtgccgg caccggtgcc
     1081 ggcaagcgcg aaggttccgg tcaggttgcc gatgcgattc gcacccgtca ccacaccgcg
     1141 caggatggcc gcacccgggc gatctccggt cgcgacgacc gtcgcgtgca gcggcatgcc
     1201 tcccagcgcc gcgctcgcat agggcagtcc gccggccggc gcgtcggccg ttgcaaccat
     1261 ctcgagcgcg cgcggctgcg accgtaggtt caggcgtccg cgcgccccca aggcgatgcc
     1321 ggcgtacgtc gcgtgcgcgt cgacgagatc gaattgctgc ccgtcgaacg ccgccagcac
     1381 cgacgttgcg tcgaacggtg ccgacgcata ggaagcgtgc ggcgcgctca acgcaatcag
     1441 ggttaacggc ttcgaaacgg gtccttccac ccgaaccccc agtcgcgccg gaccggcgag
     1501 cggcacgccg tgcgattgat tcaaaaccgc acgcaaccgg cggaggtcgc cgttgccggc
     1561 gaccgtgaga tcgaacgacg gtgctacgaa accgtacacg cctccgccca agcggatcgg
     1621 tacgcccgca atcgtcgcgc cgacgttctc cagcagcaaa ccgtcgccg
//
LOCUS       DASUCJ010001322         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_564331, whole genome shotgun sequence.
ACCESSION   DASUCJ010001322 DASUCJ010000000
VERSION     DASUCJ010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564331"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFU90_15450"
     CDS             <1..881
                     /locus_tag="VFU90_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEU5481240.1"
                     /translation="DNPNGTAPGFVAFAPGEKFVACMPGVPREMKPMLERRLLPFLRE
                     RYGDAGAIYTRVLHTVGIGESEIDRRIEELFRNAENPKIAVLAHEGRADVKIMAKSAS
                     AAAAEAAIAPLQAEIERRLAGFVFGTDAETLESAIHRLLQARGRHVATAESCTGGRIA
                     AALTQTPGSSRSFLGGVVAYDNAVKIRELGVDADVLERDGAVSENVALAMARGARTRL
                     GADVALATTGIAGPDGGSAEKPVGLVWFALDAAGGYSRAWRMQLRGERETIQGRATTV
                     GLGLLWKHLTAEGEGT"
     gene            complement(878..>1174)
                     /locus_tag="VFU90_15455"
     CDS             complement(878..>1174)
                     /locus_tag="VFU90_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481241.1"
                     /translation="RTAVASGPSITDLTLYAIDGDETSELECKPSPYAGFASINPSVP
                     PLMELYDEFVKAIERERNVLPTFDQAVATQEVLAAVGYVPGTAAGDELSESAPD"
BASE COUNT          194 a          362 c          422 g          196 t
ORIGIN      
        1 tcgacaatcc caacggcacc gcgccgggat tcgtggcgtt tgcgcccggc gagaagttcg
       61 tcgcttgcat gccgggcgtg ccgcgcgaga tgaaaccgat gctggagcgg cgtctgcttc
      121 cgttcttgcg cgagcgttac ggcgacgccg gcgcgatcta cacccgcgta ctgcacacgg
      181 tcggcatcgg cgaatcggag atcgatcgcc gcatcgaaga gctgttccgc aacgccgaga
      241 acccgaagat cgcggtgctc gcgcacgaag gtcgcgccga cgtgaagatc atggccaaaa
      301 gcgcttcggc tgccgcggcc gaagcggcta tcgctccgtt gcaagccgag atcgagcgcc
      361 ggctggcggg tttcgttttc ggtaccgacg cggaaacgct ggagagcgcg attcatcgtc
      421 tgctgcaggc gcgggggcgc cacgtcgcaa ccgccgagtc gtgtaccggc ggccggatcg
      481 ccgccgcgtt gactcaaacg ccggggtcgt cgcgaagttt tttgggaggc gtggttgcgt
      541 acgacaacgc cgtgaagatt cgcgagctcg gcgtggatgc ggacgtgctc gaacgcgacg
      601 gcgctgtaag cgagaacgtg gcactcgcga tggcgcgcgg cgcgcggacg cggctgggtg
      661 ccgacgtcgc cttggcaacg accgggattg cgggtcccga cggcggcagc gccgagaaac
      721 ccgttgggct ggtatggttt gcgctggacg cggcgggcgg atattcgcgc gcctggcgga
      781 tgcagcttcg cggcgagcgg gaaacgatcc agggccgcgc gacgaccgtc ggactcggcc
      841 tgctctggaa acacttgacc gcagaaggcg aaggaactta gtcgggtgcc gactcgctca
      901 gctcgtcgcc ggcggcggtg ccgggaacgt aaccgaccgc cgcgagcact tcctgcgtcg
      961 caacggcctg atcgaacgtc ggcagaacgt tgcgttcgcg ttcgatggcc ttgacgaatt
     1021 cgtcgtacag ctccatcaga ggcggaacgc tcggattgat cgacgcgaag cccgcgtacg
     1081 gcgacggttt gcattccagc tcgctggttt cgtcaccgtc gatcgcatag agcgtcaggt
     1141 cggtgatgct cggaccgctc gcgacggcgg tgcg
//
LOCUS       DASUCJ010001323         1494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1313108, whole genome shotgun sequence.
ACCESSION   DASUCJ010001323 DASUCJ010000000
VERSION     DASUCJ010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313108"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1398)
                     /gene="glmM"
                     /locus_tag="VFU90_15460"
     CDS             complement(25..1398)
                     /gene="glmM"
                     /locus_tag="VFU90_15460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5481242.1"
                     /translation="MVSVSGVRGRVGEALTPEVVTRFASGFGAWARTKWSQSPRTSTR
                     CRVVVGRDSRVSGPMFHRATIAALQSVGCDVIDVGLAPTPSIQLAVEHHRAAGGLAIT
                     ASHNPIEWNALKFIGPSGLFLDGAEGTAMRGVVESTIPRATWDALGEVESDDRAIARH
                     IDAVLSLPFVKVDAIRKRKFRVALDCAHGAGGAIVPELLERLGCDVHAINVETDGRFT
                     RPPEPVASNLGDLERLVRDSKADVGLATDPDVDRLALVSDKGTAIGEDWTLALAARLV
                     LRYRRGPVVANLSTSRIVDDVAREAGARVVRAPVGEVNVAVRMRTEGAAIGGEGNGGV
                     ILSEVHLGRDAPVGVVLLLQMLLEDNAPLSSIVARYPQYVIVKEKLDRPNAPLDAVYD
                     ALRQGFSDAEADTQDGLRLSWVDRWVHVRPSGTEPIVRVIAEAPSAAAARELVARSRV
                     PLDGLGR"
BASE COUNT          277 a          553 c          417 g          247 t
ORIGIN      
        1 cacatgtggt tgaacctcgg ttagtcatcg tcccaacccg tctagcggaa cacgcgaccg
       61 cgcgaccagc tcgcgcgccg ccgccgcgct cggtgcttcc gcaatcaccc gtacgatcgg
      121 ctcggtgcct gatggccgca cgtgcaccca ccggtctacc cacgacagcc gcagcccgtc
      181 ctgcgtatcc gcctcggcgt cgctaaagcc ctgtcgcaac gcatcataca ccgcgtcgag
      241 cggcgcgttc ggccggtcca gcttctcctt cacgattacg tattgggggt acctggccac
      301 tatggacgac agcggcgcgt tgtcctccag caacatctgc agcaagagta ctacaccgac
      361 cggagcgtcc cgacccaaat gcacttccga cagaatgact ccgccgttcc cctcgccgcc
      421 gatggcggcg ccttctgtgc gcatacgcac cgcgacattc acttcgccta ccggcgcgcg
      481 cacgacgcgc gccccagcct ctcttgccac gtcatcgacg atccgactgg tcgatagatt
      541 cgcgaccact ggtccgcgcc tgtatctgag cacaagccgg gccgcgagcg ccaatgtcca
      601 gtcttcacca atcgccgtgc ccttgtccga cacgagcgcc aaccggtcga cgtctggatc
      661 cgtcgccaaa ccgacgtcgg ctttggagtc ccgcacgagc cgttccaagt cgcccaggtt
      721 cgatgcaacc ggctccggcg gcctcgtgaa tcgaccgtca gtctcgacgt tgatcgcgtg
      781 cacgtcgcaa cccaagcgct cgagcagttc gggaacgatc gcgccgcccg cgccatgcgc
      841 acagtcgagc gcgacgcgga actttcgctt ccgaatcgcg tccactttta cgaacggcag
      901 cgacaacacc gcgtcgatat gccgcgcgat cgctcgatcg tcgctctcca cctcgccgag
      961 cgcatcccac gtcgcgcgcg gaatcgtgct ctcgaccaca ccgcgcatcg ccgttccttc
     1021 ggcgccatcc agaaaaagtc cggacgggcc gatgaacttg agcgcgttcc actcgatcgg
     1081 attgtggctt gccgtgatgg ccaatccgcc ggcggcccga tggtgctcga ccgcgagctg
     1141 aatggacggc gtgggtgcaa gtccgacatc gatcacatcg cagccgacgg actgcagcgc
     1201 cgcaatcgtc gcgcgatgaa acatcggacc tgacacacgc gagtcccgcc ccaccacgac
     1261 gcgacaccgc gtcgatgtac gcggcgattg ggaccacttg gtgcgcgccc aggcgccgaa
     1321 gcctgatgcg aagcgcgtca cgacttccgg cgtgagcgct tcaccgacgc gcccgcgcac
     1381 gcccgacacg ctaaccatca gtccctcgta cacgctgtaa cctccatcac ggtccgagac
     1441 aaagctatgc gaacgctcgc aacaagaata acgagaagtc gtggactgtg acgg
//
LOCUS       DASUCJ010001324         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1466151, whole genome shotgun sequence.
ACCESSION   DASUCJ010001324 DASUCJ010000000
VERSION     DASUCJ010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466151"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFU90_15465"
     CDS             complement(<1..443)
                     /locus_tag="VFU90_15465"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5481243.1"
                     /translation="MIKVATDAGGTFTDLVAFEETSGKIYVGKALTTPKDPSLGVMHS
                     IAQSRESGLVAEAISFFVHGGTTVINAITERKGVRTALVTTEGFRDVIAIGRGNRPDL
                     YNLHSTPPEPFVLRHLRLEVAERVDAKGNVRVPLSIASVDAVAEV"
     gene            complement(440..>1178)
                     /locus_tag="VFU90_15470"
     CDS             complement(440..>1178)
                     /locus_tag="VFU90_15470"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5481244.1"
                     /translation="WYYEGVAQASDLVWKALAPLVPERFSAGSYLSLCATYFSGAAAD
                     GLFVHIEPQNGGWGATDARDGASGLIAVTDGDTYNYSIELLEAKFPLLIRRYEYNVEN
                     GVGAGRFRGGFGLLREYETESDGVILHASYGRTATPPWPIGGGSPGSLNGIEVVRGTT
                     RQRLTRPSDFPLDRGDRVRICTGGGGGWGEPQTRDPAAVARDVRDGFVTVADAAAIYG
                     VVIDPDALKVDPAATDKLRANRSGGAR"
BASE COUNT          211 a          393 c          381 g          193 t
ORIGIN      
        1 atcacctcgg cgacggcatc gaccgaggcg atcgacagcg gcacgcgcac attacctttc
       61 gcgtcgacgc gctcggcgac ttcgagacga agatgccgca acacgaacgg ctcgggcggc
      121 gtggaatgaa gattgtagag gtcgggacgg ttgccgcggc cgatcgcaat cacatcgcga
      181 aagccttcgg tcgtgaccaa agccgtgcgc acgcccttgc gttcggtgat ggcgttgatc
      241 acggtcgttc cgccgtgaac gaagaacgat atcgcctcgg ccacaagccc gctctcgcgg
      301 ctttgtgcga tggagtgcat gacgccgagc gacgggtcct tcggcgtggt cagggccttg
      361 ccgacgtaga tcttgccgga cgtctcttcg aaggcgacga ggtcggtgaa cgtgccgccg
      421 gcgtcggtgg cgaccttgat catcgcgcac cacctgagcg gttcgcgcgc agcttatcgg
      481 tggccgcagg gtcgactttc agcgcatccg gatcgatcac gaccccgtag atcgcagccg
      541 cgtctgcgac cgtcacgaat ccgtcgcgca catcgcgggc gacggccgcg ggatcgcggg
      601 tctgcggctc accccatccg ccgccgccgc cggtgcagat gcgaacgcga tcgccgcggt
      661 cgagcggaaa atccgacgga cgcgtgagcc gctgacgcgt tgtcccccgc accacctcga
      721 tgccgttgag gctgcccggc gagccgccgc cgatcggcca aggcggggtt gcggtacggc
      781 cgtaactggc gtgcagaata acgccgtcgc tctcagtttc gtattcgcgc aggagaccga
      841 agccgccgcg gaaacgtccc gcgcccacgc cgttttcgac gttgtattca tagcggcgga
      901 tcagaagcgg gaatttcgcc tccaacagct cgatcgaata attgtaggtg tcgccgtcgg
      961 tgacggcgat caagccgctc gcgccatcgc gggcgtcggt cgcgccccag ccgccgtttt
     1021 gcggctcgat gtgaacaaac aacccgtcgg ccgccgcacc tgagaaatag gtcgcgcaca
     1081 agctcagata actgccggcg gagaagcgct cgggcaccaa cggcgccagc gccttccaga
     1141 ccagatcgga ggcctgcgcc accccttcat aataccag
//
LOCUS       DASUCJ010001325         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_961535, whole genome shotgun sequence.
ACCESSION   DASUCJ010001325 DASUCJ010000000
VERSION     DASUCJ010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961535"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFU90_15475"
     CDS             <1..345
                     /locus_tag="VFU90_15475"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU5481245.1"
                     /translation="ARYLFDGLTSRALPLVIVSAVCGLASLYALARANRRFARPLAIG
                     AVTTVVVGWGVAQWPYLLPQTLKVSAGAAPDPTLATVLVVFALAAVLILPSLGWLYYL
                     DQRSTLTGEGAD"
     gene            404..>1082
                     /locus_tag="VFU90_15480"
     CDS             404..>1082
                     /locus_tag="VFU90_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481246.1"
                     /translation="MFSTLSTRTTALIRAIRDGDDRMVEDAVRALSQSRRILAPLAFA
                     IGAFVMLFQGVKLLVSNWRLTLVQLLPAIWIWVAMFDLRAHAFRGDSFRVLRGPVLIP
                     IILAIAAITAATFFLNAVFAFAIATPPPPRIRPAFRHARAHLAVVLGSGAVVGLLLGF
                     SAVVVDRWGAIWFAICMTITIGIMCVCYVAIPSRLIGVAPKRSRRDKLTASAIGGALG
                     AVICTPPH"
BASE COUNT          137 a          376 c          342 g          227 t
ORIGIN      
        1 gcgcgatacc tgttcgacgg tctcacgtcg cgtgcgctgc cgctcgtcat cgtctcggcg
       61 gtgtgcggcc tggcgtcgct gtacgcgctc gcgcgtgcga atcggcgatt cgctcgtccg
      121 ctcgcgatcg gcgcggtcac gaccgtcgtc gtgggatggg gagttgccca gtggccctat
      181 ctcctcccgc agaccctgaa ggtgtccgcc ggcgcggcgc ccgatccgac gctcgcaacg
      241 gttctcgtcg tgttcgcgtt ggcggccgtg ctcatcctgc cgtcacttgg gtggctctac
      301 tacctcgacc agcgcagcac cctgaccggg gaaggcgccg actgacgaag ggctgcgcgc
      361 cgctcgcaca cgcagcccgc gatccggcaa tgtgttgtcg atcatgtttt ccacgttgtc
      421 gactcgaacg actgcgctga tccgagcgat ccgcgacggt gacgaccgga tggtcgaaga
      481 cgcggtgagg gcgctcagcc aatcgcggcg aatcttggcc ccactcgcat ttgcgattgg
      541 cgcgttcgtg atgctcttcc agggcgtgaa gctgctcgtg tccaactggc ggctcacgct
      601 cgtgcaactt ctgcccgcga tatggatctg ggtcgcaatg ttcgatctca gagctcacgc
      661 gtttcgcggc gattcgtttc gcgtcctgcg cgggcccgtg ctcatcccga tcatcctcgc
      721 catcgccgcg atcactgctg cgaccttctt tctgaacgcc gtcttcgcgt tcgcgatcgc
      781 aacaccgcct ccgccccgca tccgtcccgc gttccggcac gcacgagcgc acctcgccgt
      841 cgtgctcgga tcgggagccg tggtcgggct gctgctcggc ttctccgcgg tcgtcgtcga
      901 tcgctggggc gcaatctggt tcgcgatctg tatgacgatc acgatcggca tcatgtgcgt
      961 ctgttatgtc gcgatccctt cgcgtctgat cggcgtggcg ccgaaacggt cccggcgcga
     1021 caagctcacg gcatcggcga tcggcggtgc gctcggagcc gtgatctgca cgcccccgca
     1081 tc
//
LOCUS       DASUCJ010001326         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_411156, whole genome shotgun sequence.
ACCESSION   DASUCJ010001326 DASUCJ010000000
VERSION     DASUCJ010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411156"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFU90_15485"
     CDS             complement(<1..569)
                     /locus_tag="VFU90_15485"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5481247.1"
                     /translation="MLRVGIDVGGTFTDVAAFDDAGGELVTLKVPTTPRDPERGVAAS
                     LEQLLANYSGAPAAIGFLGHSTTIATNALLGQVGLELPRVALVTTEGFRDVIEIGRQN
                     RSDVYDLFVQRPRPLVARGDRFTVRERIDERGAVVQPLDEASLADACERLRSCAGLGA
                     VAVCLLNSYANGCHERRVAEAVAEAVPGAT"
     gene            complement(609..974)
                     /locus_tag="VFU90_15490"
     CDS             complement(609..974)
                     /locus_tag="VFU90_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481248.1"
                     /translation="MKAPLSLSVLFALLFGMAAAGDAATTAVTLQPSSLTFKQAVAAC
                     SGALGSITFTLGHARVAGLPDVVEQVDTSSSGIDTITFSNETTGKSATAVANGHKSTV
                     SAKNVQVKWKNQLACVMPD"
     gene            1100..>2025
                     /locus_tag="VFU90_15495"
     CDS             1100..>2025
                     /locus_tag="VFU90_15495"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481249.1"
                     /translation="MVPHSPRSGRTAFQLLLAVSALALAACSSPTEGAGRFVPISPAA
                     GDAARGIAALVGDARFAASGKIQHVVVIVQENRSFDDLFQGYPGADTQAYGYAKDGRK
                     IDLKPASLATAWDIGHDSQNYYLACDGTGKLPGTRCKMDGFNQEYLSCGAPHTPQPCP
                     HRNPQYGYVPHDEAKPYFDIAHDYVLADRMFPSNFDGSSFISHQYIIAGQAGASIDYP
                     DNVWGCDGGPTDKIGTITRLRAPGPRIRVCYGNRTLGDELDEAHLTWRYYASRLDRDG
                     DIWSAYQAIKHIRYGPDWKHHVISPQTRFFNDL"
BASE COUNT          354 a          668 c          668 g          335 t
ORIGIN      
        1 gtcgcacctg gaacggcttc ggcgaccgcc tcggccacgc ggcgctcgtg gcagccgttt
       61 gcgtatgagt tgagcaaaca cacggctacg gcgccgagcc cggcgcacga ccgcaggcgt
      121 tcgcaggcat ccgccagcga agcttcgtcg agcggctgca cgaccgcgcc gcgctcgtcg
      181 atgcgttcgc gcacggtgaa acggtcgccg cgcgcgacca gcggacgcgg gcgctgcacg
      241 aaaagatcgt aaacgtcgct gcgattctgg cggccgattt cgatgacgtc gcgaaacccc
      301 tcggtcgtta ccagcgcgac gcgcggcagc tcgagaccga cttgcccgag cagcgcgttg
      361 gtggcgatgg tcgtcgaatg accgagaaac ccgatcgccg caggcgcgcc ggagtagttg
      421 gccagcagct gctcgagcga agcggcgacg ccgcgctccg ggtcgcgcgg cgtcgttggg
      481 actttcagcg tgaccagttc gccgcccgca tcgtcgaacg cggcgacgtc ggtgaaggtg
      541 ccgccgacgt cgatgcctac ccgcagcacg cggccgctac aatcgaaagc gcgattcgcc
      601 tcgccgcgtc aatcgggcat gacgcaggcg agctgattct tccactttac ctggacgttc
      661 ttggcggaaa cggtgctctt gtgaccgttg gcgacggcgg tggccgactt gcccgtcgtt
      721 tcgttgctga acgtaatcgt gtcgattccc gaggacgacg tgtcgacttg ctcgacgacg
      781 tccggcaacc cggcgacgcg cgcgtgtccg agcgtgaacg taatgcttcc gagggctccc
      841 gagcacgccg cgacggcctg cttgaacgta agcgacgatg gctggagggt gacggcggtc
      901 gtcgcggcgt cgccggcggc ggccatgccg aacaggagcg cgaacaatac cgaaaggctg
      961 agcggagctt tcatgctggc ggcgactccc caagacgagc ggtggcggca gaagtttgta
     1021 cgccggcccg gcagaacctt tagcggcgtg cgggccggca tgatgcgaac cgatacgccc
     1081 agaaccacgt tacgtccgta tggtacctca ctcgccccgc agcggccgca cggctttcca
     1141 gctgctgctc gccgtatcgg cgctcgcgct cgccgcctgt tcgtcgccga cggaaggcgc
     1201 cgggcggttc gtgcccattt cgccggcggc cggcgacgct gcgcgcggca tcgcggcgct
     1261 ggtcggcgac gcgcggtttg cggccagtgg aaaaatccag cacgtcgtcg tgatcgttca
     1321 ggaaaatcgc agcttcgacg atttgtttca gggctatccc ggtgcggata cgcaggccta
     1381 cgggtatgcg aaagacggcc gcaagatcga tctcaagccc gcgtcgctcg cgactgcctg
     1441 ggacatcggg cacgattcgc aaaattacta tctcgcttgc gacggcaccg gaaagcttcc
     1501 gggtacccgc tgcaagatgg acgggttcaa ccaggagtat ctctcgtgcg gtgcgccgca
     1561 tacgccccag ccgtgcccgc accggaatcc gcaatacggt tacgtaccgc acgacgaagc
     1621 caagccgtac ttcgacatcg cgcacgacta cgtgctcgcc gaccggatgt tcccatcgaa
     1681 cttcgacggg agcagcttca tctcgcatca gtacatcatc gcggggcaag ccggcgcgag
     1741 catcgattat ccggacaacg tgtggggctg cgacggcggg cctaccgaca agatcgggac
     1801 gatcacgcgg cttcgcgcgc cgggtccgcg catccgcgtt tgttacggta atcggacgct
     1861 cggcgacgag ctggacgagg cccacctcac gtggcggtac tacgcctccc ggctggaccg
     1921 cgacggggac atttggagcg cctatcaggc gatcaagcac atccgctacg ggcccgattg
     1981 gaagcaccac gtcatcagcc cgcagacgcg ctttttcaac gatct
//
LOCUS       DASUCJ010001327         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1007548, whole genome shotgun sequence.
ACCESSION   DASUCJ010001327 DASUCJ010000000
VERSION     DASUCJ010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007548"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFU90_15500"
     CDS             <1..561
                     /locus_tag="VFU90_15500"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU5481250.1"
                     /translation="VSRLCGYVAAALIAVGVLVVCQMVFVRFVLNENTIWQTDFVTYS
                     LVAATFVGSPYVLMLKGHVNVDVLPLHLRPRKRFWLAVFSMLAAIAFCAILTVLTAEY
                     WLEAWRQHWLSNTMWRVRLWIPYASMPVGLGVLTLLYVADFYELVTHRTAPFGIVDHP
                     TDGEGKAEGAAPAVDAEAVRAGMGGS"
     gene            602..>1024
                     /locus_tag="VFU90_15505"
     CDS             602..>1024
                     /locus_tag="VFU90_15505"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU5481251.1"
                     /translation="MSPSTQGLIVLVVTLLVLLSGAPVAFGLGLLSIVFIAIFQGLDA
                     LHVVAETFFSGLNDFTLVSIPMFVMMGAAIGSSPAGKDLYEALDRWLYRLPGGLVLAN
                     LGACAIFAALTGSSPACCAAIGKMGIPEMRRRGYPDDVA"
BASE COUNT          126 a          333 c          353 g          212 t
ORIGIN      
        1 gtttcgcggc tgtgcggcta cgtcgcggcg gcgctgatcg cggtcggcgt gctcgtcgtc
       61 tgccagatgg tgttcgttcg cttcgtgctg aacgagaaca cgatctggca gaccgacttc
      121 gtcacctaca gcctcgtcgc ggcgacgttc gtcggcagtc cttacgtgct gatgctgaag
      181 gggcacgtga acgtcgacgt gctgccgctg cacctgcgcc cgcgcaaacg gttctggctc
      241 gcggtgttct cgatgctcgc cgccatcgcg ttctgtgcga ttctcaccgt gctgacggcg
      301 gagtactggc tcgaggcctg gcggcagcat tggctctcga acacgatgtg gcgcgtccgg
      361 ctgtggattc cgtatgcatc gatgccggtc ggcctcgggg tgctgacgct gctgtatgta
      421 gccgacttct acgagctcgt gacgcaccgc acggcgccgt tcggcatcgt cgatcatccg
      481 acggatggcg agggcaaggc cgaaggcgct gcaccggccg tcgacgcgga agccgtgcgc
      541 gccgggatgg gtggatcgtg agctcgatgc ccgcgccacc cgcacacgaa cagaggctgt
      601 cgtgagtccg tcgacccagg gcctgatcgt tctcgtcgtc acgctgctcg tgctgctctc
      661 gggcgcgccg gtcgcgttcg ggctcggcct gctgtcgatc gtcttcatcg cgatcttcca
      721 gggcttggac gcgctgcacg tcgtcgcgga gacgttcttc tcgggtctca acgacttcac
      781 gctcgtgtcg atcccgatgt tcgtgatgat gggcgcggcg atcgggtcgt cgccggccgg
      841 caaggatctc tacgaggcgc tcgatcggtg gctgtatcgg ctgcccggcg gcctcgtgct
      901 cgcgaacctc ggtgcctgcg cgatcttcgc cgcgctcacc ggctcctcgc cggcgtgctg
      961 tgccgcgatc ggcaagatgg gcattccgga gatgcgccgg cgcggctatc cggatgacgt
     1021 cgcc
//
LOCUS       DASUCJ010001328         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1129840, whole genome shotgun sequence.
ACCESSION   DASUCJ010001328 DASUCJ010000000
VERSION     DASUCJ010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129840"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFU90_15510"
     CDS             complement(<1..729)
                     /locus_tag="VFU90_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481252.1"
                     /translation="MLSADRHGSPQDSRRYAVRQAIAVNVDVFGPDAETEIVQPRLRS
                     GQRERHPVDDESGSYRLHGHEVHAWVAEKPASELAVRLVVEFRLRRALFDPAIAHQRD
                     LVGERHCLRLVVRDVHDRLPNVLSQLFEGPDQRQFRVRLDVGRRLVKQRDGGAARDGA
                     THRDLLARPEVKAIDVQPAGVAQFEDVDRFLDPSAPLCRRDGAVVRKRQLYILGHVQR
                     PVHDRILEDHRYIALAGGKRRHVAI"
BASE COUNT          197 a          334 c          338 g          168 t
ORIGIN      
        1 gatcgcgacg tgacggcgct tgccccccgc cagcgcgatg tagcgatggt cttccagtat
       61 ccggtcatgt accggacgct gaacgtgacc gagaatatag agttgccgct tgcgaacgac
      121 cgcaccgtca cgccggcaga gcggcgccga cgggtcgagg aagcgatcga catcctcgaa
      181 ctgggcgacg cccgccggct gcacatcgat cgccttgacc tcggggcgcg ccagaaggtc
      241 gcgatgggtc gcgccatcgc gcgccgcgcc gccgtcacgc tgtttgacga gccgacgacc
      301 aacgtcgagg cgcacgcgaa actggcgttg atccgggcct tcaaacagct gcgaaagcac
      361 gttcggcaaa cgatcgtgta cgtcacgcac gaccagacgg aggcaatgac gctcgccgac
      421 caaatcgcgt tgatgcgcga tggcaggatc gaacagtgcg cgccgcagcc tgaactctac
      481 gaccagccga acagcgagtt cggaggctgg tttctcggca acccaggcat gaacttcatg
      541 accgtgcagc cggtaggagc cggattcgtc gtcgacgggg tgccgttcac gctggccgct
      601 ccgcaaacgg ggctggacga tctccgtttc ggcatccggc ccgaatacat ccacattgac
      661 agcaatcgcc tgcctgacag cgtaccggcg cgagtcttgc ggagagccgt ggcgatcggc
      721 ggacagcatc tgctcacgct cgacgtgggt gggagccgca tcaaggcgaa ggtctcgtcg
      781 gctgcgggcc cttcgcggac tgcgccggag gtcggcgcga acgtctttat cgtatgtccc
      841 atcgaacacg cgatcttgtt ccgccgtggc gaacgagtga tcgagccgct gcgccgcctg
      901 gattcgtcga cctagtctgg atgtcgagct gctcctcgcg cggggccgga tcaagcggca
      961 gccggggtga gaccggaaat cacgaccatg ctggaaagac tgccgtaatg acattggctg
     1021 cgagcccccg caaccac
//
LOCUS       DASUCJ010001329         1465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_564453, whole genome shotgun sequence.
ACCESSION   DASUCJ010001329 DASUCJ010000000
VERSION     DASUCJ010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564453"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFU90_15515"
     CDS             complement(<1..1452)
                     /locus_tag="VFU90_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481253.1"
                     /translation="MAQSKARDSRVATKTGRAPALGEPRFATAWAAVVYIVATLILAY
                     PALVGRFLVNPSSDQYIGGYAFREFAAASLRAGHGFPLWNPYLFGGMPYVAAMSGDIF
                     YPTFLMRMIMPTDVAMTWAFIIHIILAGLFTYILLRACRFSFYGALTGGLVYMMGGPI
                     SSYVSPGHDGKLYVSALLPLVLFLLVRAIRDGKAWSYGALAIVIGLACLSPHPQLFQY
                     MLLVSGAFAVWLAFGSDNAPALERRLAFKRLGGSLVAVCLGTVMGAIQYTSVLQYVPW
                     SPRAGGLSGWAHATSYSFPPEELINTYIPQFTGMFDHYWGQNGIHLHSEYLGVVALML
                     ALLGVVYAWRPDRRSFGRFWFVTFIVALLWSLGGFTPFYHIVYALVPGTKYFRAPSTM
                     IFVVGLCVAIFAAFGAERLERADVTRQYAVGWLIAAAVVLLLGVTGMLTNIASTIAPP
                     DHQEGVALNAPLLLVGAVRTAVFTAIAAGLLLVL"
BASE COUNT          305 a          445 c          500 g          215 t
ORIGIN      
        1 aaggaccagc aggaggccgg ccgcgatcgc ggtgaagacg gccgttcgca cggcgccgac
       61 taacaggagc ggcgcattca acgcgactcc ttcctggtga tcgggcggcg cgatggtgga
      121 ggcgatgttg gtcaacatgc cggtgactcc gagcagcagc acgaccgcgg ccgcaatgag
      181 ccagccaacg gcgtattgtc gcgtcacgtc ggcccgttcc aggcgttcgg cgccgaacgc
      241 cgcgaagatg gcgacgcaca gtcccaccac gaaaatcatg gtgctcggcg cccgaaaata
      301 cttcgtcccg ggcacgagcg cgtagacgat gtgatagaac ggcgtgaacc cgcctaacga
      361 ccagaggagc gcaacgatga acgtgacgaa ccagaatcgc ccgaaggatc ggcggtccgg
      421 cctccacgcg tagacgacgc cgagcagagc gagcatgagt gcaaccacgc ccagatattc
      481 gctgtgcagg tggatcccgt tctggcccca atagtggtcg aacatcccgg tgaattgcgg
      541 gatgtaggtg ttgatcagtt cttcgggcgg gaatgaatac gacgttgcgt gcgcccagcc
      601 gctcaagcct cctgcacgcg gcgaccacgg gacgtactgc agcacagatg tgtattgaat
      661 ggcgcccatg accgtgccca ggcacacggc cacgagcgag ccgccgagac gcttgaatgc
      721 gaggcgtcgt tccaacgccg gcgcgttgtc gcttccgaac gcgagccaga ctgcgaacgc
      781 gccgctcacg agcagcatgt actggaagag ctgcggatgc gggctcagac acgcgagtcc
      841 gatgacgatg gcgagcgcgc cgtacgacca ggctttgccg tcgcggatcg cgcggacgag
      901 caggaacagc acgagcggca ggagcgcgct cacgtagagt ttaccgtcgt gccccggcga
      961 gacgtacgac gagatcggcc cgcccatcat gtagacgagc ccgccggtca gcgcgccgta
     1021 gaacgagaag cggcacgcgc gcagcaggat gtaggtaaag agtccggcga ggatgatgtg
     1081 gatgatgaat gcccacgtca tggcgacgtc ggtgggcatg atcatgcgca tcaggaatgt
     1141 cggatagaaa atgtcgccgc tcatggcggc gacgtatggc atgcctccaa acaggtacgg
     1201 gttccacaac gggaacccgt gtccggcgcg cagcgacgct gcggcgaatt cgcggaacgc
     1261 gtagccgccg atgtactggt cgctcgacgg attgaccagg aaccgcccga cgagcgccgg
     1321 ataggcgagg atgagcgtcg cgacgatgta cacgacggcc gcccaggcgg tggcaaatcg
     1381 cggctcgcct aacgccgggg cgcggccggt cttggtcgca acgcggctat ctcgtgcttt
     1441 cgattgggcc atcggaacgt ctcgg
//
LOCUS       DASUCJ010001330         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1481377, whole genome shotgun sequence.
ACCESSION   DASUCJ010001330 DASUCJ010000000
VERSION     DASUCJ010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481377"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..>1222)
                     /locus_tag="VFU90_15520"
     CDS             complement(180..>1222)
                     /locus_tag="VFU90_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481254.1"
                     /translation="TFTAGFICNIPRAATADGNDPVRPARGGVYGHGLLGSINEIESF
                     SQFINQYDVVLCATPEIGMADEDLGNVFSAIQDFSTFGSIPDRLQQGILNMQFLARLL
                     KDPRGFGNNPAFRVGTPAASAILANQVFYNGNSQGGIFGGAATAISKEWTRAVLGVPG
                     MNYGELLPRSVDFDPFLPLVSASYPDARLHTLIVALIQILWDRGEPDGYAQHMIRDPY
                     PGTPNHQVLLIEAFGDHQVANISTETEARTIGALVRQPAIAPGRSNDVTPMWGIPAVP
                     SSPFDGSVLELWDFGTPAPPIASLPPESPQYGADPHGAARNVPAVRDQVSQYLQPAGV
                     FVDECGTDPCHP"
BASE COUNT          215 a          387 c          416 g          204 t
ORIGIN      
        1 ccagcacaag ctcaacgcgc tcgccgacgg cctcgccgat ctgatggcgt tcgtcgagca
       61 acaaggcgaa gggcgcgacc tcgcacgcga acttctcgag ctgcgcaccg cggtcgggct
      121 cgaacagcac gagagcacgt cgcggaacaa gcccgcacgc aactgatctc gtcatctcgt
      181 cacgggtggc acggatcggt gccgcattcg tcgacgaaca caccggccgg ttgcagatac
      241 tgcgagacct ggtcccgtac cgccggcacg ttgcgcgcgg cgccgtgcgg gtcggcaccg
      301 tattgcggcg attcgggtgg caagctcgcg atcggcggtg ccggcgttcc gaaatcccag
      361 agctcgagca cggacccgtc gaacggactc gacggaaccg ccggaatgcc ccacatcggg
      421 gtgacgtcgt tgctgcgacc cggcgcgatc gccggctgtc gaaccaacgc gccgatcgtg
      481 cgtgcttccg tctcggtcga gatgttcgcg acctggtgat cgccgaaggc ctcgatcagc
      541 agtacctggt ggttcggcgt acccggatac ggatccctga tcatgtgctg cgcgtagccg
      601 tccggctcgc cacggtccca aaggatttga atcaacgcga cgatcaaggt gtgcagccgc
      661 gcgtccggat acgacgccga gacgagcggc aggaacggat cgaagtctac gctgcgcggc
      721 aggagttcgc cgtagttcat gccgggaacg ccgagtacgg cgcgggtcca ctctttcgag
      781 atcgcggtcg cggcgccacc gaagatcccg ccttggctgt tgccgttgta gaagacctgg
      841 ttcgccagga tggcggacgc ggcaggtgtg cccacgcgga acgcggggtt gttgccgaaa
      901 ccgcgcggat ccttgagcag tcgcgcgagg aattgcatgt tcaggatgcc ctgctgcagg
      961 cggtcgggaa tcgagccgaa cgtcgagaaa tcctggattg ccgagaacac gttgccgagg
     1021 tcctcgtccg ccatgccgat ctcgggtgtc gcgcaaagca cgacgtcgta ttggttgatg
     1081 aactggctga agctctcgat ctcattgatg ctgccgagca ggccatggcc gtacacgccg
     1141 ccacgggccg gacggacggg gtcgttgccg tccgcggtcg cggcgcgcgg gatgttgcag
     1201 atgaagccgg ccgtgaacgt cc
//
LOCUS       DASUCJ010001331         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1404994, whole genome shotgun sequence.
ACCESSION   DASUCJ010001331 DASUCJ010000000
VERSION     DASUCJ010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404994"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFU90_15525"
     CDS             complement(<1..197)
                     /locus_tag="VFU90_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481255.1"
                     /translation="MILAGLFGRRHPDDPVAVQWLNAATQWIQAHWTVLAVIIPVAGL
                     IVAGVVNHYLAVARDNRARRVS"
     gene            complement(214..>1609)
                     /locus_tag="VFU90_15530"
     CDS             complement(214..>1609)
                     /locus_tag="VFU90_15530"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU5481256.1"
                     /translation="LTRGGKIKAPITPPPLNNRGKYVASLQKLSKWLGEKAEEAGAQV
                     IPGFPGQELLWDDDDRVVGVRTGDKGLDHEGKPKSNYEPGADILAKVVVLGEGPRGTL
                     AKQAIPRLGLDEGREPQVYAAGIKELWQLPDDRFKAGTVVHTLGFPLPAETFGGGFIY
                     GMAGNVLDIGHVTGLDYRDPTTDPHNELQRMKTHPAIARLLDGGTLIRYGAKAIPEGG
                     LFAMPRPYGDGLLLIGDTGGFLNGMRLKGIHLAMKSGMMAAETVWEALQSGAWDAGSL
                     SSFQRRFEQSWAYAEMRSSRNFHQGFKRGRWPGMISAGLSTITGGRGFGVADKLPGEE
                     GFDVMAKAGYRPKETPRATADNVLTFDKLTDVYNSGTMHEEDQPSHLKVADTNICRDR
                     CTVEYGNPCQYFCPAAVYEPMFEKTASGSIDGRLQINFTNCVHCKTCDIIDPYQIITW
                     VPPQGGEGPVYTGM"
BASE COUNT          271 a          520 c          507 g          311 t
ORIGIN      
        1 gaaacgcggc gcgcgcgatt gtcgcgcgcc accgcgaggt agtgattgac gacgccggcg
       61 acgatcagac cggcaaccgg gatgatcacc gcaaggaccg tccagtgagc ctgaatccac
      121 tgcgtcgcgg cgttcagcca ctgtaccgcg acgggatcgt cgggatgacg gcgaccgaac
      181 agaccggcca aaatcacgcg cacgttgcgc tccctacatt cccgtgtaga cgggaccctc
      241 accaccctgc ggcggcaccc aggtgatgat ctgatacggg tcgatgatgt cgcaggtctt
      301 gcagtgcacg cagttggtaa agttgatctg caggcgcccg tcgatcgatc cgcttgcggt
      361 cttttcgaac atcggctcgt agacggccgc cggacagaaa tactggcatg gattgccgta
      421 ctcgaccgtg cagcgatcgc ggcaaatatt ggtatcggcc accttcagat gcgacggctg
      481 gtcctcttcg tgcatcgtgc cactattgta gacgtcggtg agtttgtcga aggtcagcac
      541 gttgtcggcc gtcgcgcgcg gcgtctcttt cgggcggtat ccggcttttg ccatcacgtc
      601 gaagccttct tcgcccggca gcttgtcggc gacgccgaag ccgcggccgc cggtaatcgt
      661 cgacaagccc gcgctgatca ttcccggcca gcggccgcgc ttgaaccctt gatggaagtt
      721 gcgcgacgag cgcatctccg cgtacgccca cgactgctcg aaacggcgct ggaagctcga
      781 cagcgaaccg gcgtcccaag ctcccgattg caacgcttcc cagaccgtct cggccgccat
      841 catgccggat ttcatcgcca agtgaatgcc tttgagccgc atcccgttca aaaagccgcc
      901 ggtgtcgccg atcagcagca agccgtcgcc gtaggggcgc ggcatcgcga acagcccgcc
      961 ctccggaatc gctttcgcgc cgtaccggat cagcgtgccg ccgtcgagca gccgcgcgat
     1021 ggccggatgg gttttcatgc gctgcagctc gttgtgcgga tccgtcgtcg gatcgcggta
     1081 gtcgagcccg gtcacgtgcc cgatgtcgag cacgttgccg gccatcccgt agataaaacc
     1141 gccgccgaac gtttcggccg gcaacgggaa tccgagcgtg tgcacgacgg tgcccgcttt
     1201 gaaacggtcg tcgggaagct gccacagctc tttgatgccg gcggcgtaga cttgaggttc
     1261 gcgcccctcg tcgagaccga gccgcggaat cgcttgcttt gcgagcgtac cgcgcggacc
     1321 ttcgcctaag acgacgacct tggcgaggat gtcggcgccg ggctcgtagt tcgatttggg
     1381 tttgccttcg tggtcgagcc ccttgtcgcc ggtgcgaacg ccgacgacgc gatcgtcgtc
     1441 gtcccacaag agttcctgac cgggaaagcc cggaatcact tgcgcgccag cttcctcggc
     1501 tttctcaccg agccatttgc tgagcttttg cagcgaggcg acgtacttgc cgcgattgtt
     1561 gagcgggggc ggcgtgatcg gcgctttgat ctttccgccg cgcgtgaga
//
LOCUS       DASUCJ010001332         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_732596, whole genome shotgun sequence.
ACCESSION   DASUCJ010001332 DASUCJ010000000
VERSION     DASUCJ010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732596"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFU90_15535"
     CDS             complement(<1..682)
                     /locus_tag="VFU90_15535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU5481257.1"
                     /translation="MANETFLSARPPLSEPNAMPLKVSVVIVTFFREQFLRRTLWSCL
                     GQRDVDFADVEIIVVDGSPNASARGVVDEIEEEGRQLGLTVRFIHEPRPGISHSRNAG
                     MRAAASGLVAFIDDDEEADPHWLARLLACQTQFGADVVVGPVYPMFELDRASRDPYWR
                     WYFTADSRQPSGEIAKRGAGTHNCLIRKQTCCTDEEPFDPAFGLTGGEDTRFFHSVAA
                     RGGRVIWCA"
     gene            865..>1513
                     /locus_tag="VFU90_15540"
     CDS             865..>1513
                     /locus_tag="VFU90_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU5481258.1"
                     /translation="MTIGIAVQIALQAVYFIVVTRTMGARSYGAFVSVSALSTIVAAF
                     SVWGADQLLIRTVANDQKAFSRSFGEAAIYFLVTAPVFTVAAAIGFRFLVTAEISWTA
                     IALVAVTEIVFARANYFAVNCFQAFERGRDMAMLGILLYAARVAAALIWSATTTDPTP
                     LSWAWFYMTSSFLAGAASLTLVVVKLGAPQWRARWSDWKEGGFFALQMGSFVGFRD"
BASE COUNT          267 a          482 c          473 g          291 t
ORIGIN      
        1 cggcgcacca gatgacgcgg ccgccgcgcg cggcgacgga atggaaaaag cgcgtatcct
       61 cgccgcccgt gagaccgaaa gcaggatcga acggttcttc gtcggtgcag caggtctgtt
      121 tgcggatgag gcagttatgc gtgccggcgc cgcgcttcgc gatctcgccg gaaggctgcc
      181 gggaatccgc cgtgaaatac cagcgccaat aagggtcgcg tgaggcgcga tcgagctcga
      241 acattggata gaccgggccg accacgacat cggcgccgaa ttgcgtctgg cacgccagca
      301 ggcgcgcgag ccaatgcggg tccgcctctt cgtcgtcgtc gatgaacgcg accaggccgg
      361 atgcggcggc ccgcatgccg gcattgcgcg aatgggaaat gccgggccgc ggctcgtgaa
      421 taaaccgcac cgtcagaccg agctgacgcc cttcttcttc gatttcgtcg acgacgccgc
      481 gcgccgaagc attgggcgag ccgtcgacca cgatgatctc gacatcggcg aaatcgacgt
      541 cgcgctgtcc gaggcagctc cacagcgtcc ggcgcagaaa ctgctcacga aagaaggtta
      601 cgatgacgac gcttaccttg agaggcatgg cgttcggttc cgaaaggggc ggtcgcgcgg
      661 aaagaaaggt ttcgttagcc ataaaaactc gtccaatccg gcgtcttaat cttatttccg
      721 cgccgtttcg tcatttgtta cctagaatag attggtggag acactgtagt tccttcgatt
      781 tagagcaaaa tgatcaacaa tttattacga cgcagcccgc tgcgtgcgct gctgaatccg
      841 accatcgccg gcaacaccgg cgtgatgacg atcggcatcg ccgttcagat cgccttgcag
      901 gccgtctact tcatcgtcgt gacgcggacg atgggcgcgc ggtcctatgg cgcgttcgtc
      961 agcgtctccg cgctctccac catcgtcgcg gcattctcgg tctggggcgc cgaccagctt
     1021 ctgatccgca ccgtcgcgaa cgaccagaag gcgttctccc gctccttcgg cgaggccgcg
     1081 atctattttc tggtgacggc gccggtcttc accgtggcgg ctgcgatcgg tttccgcttc
     1141 ctcgtcaccg ccgagatttc gtggaccgcg atcgcgctgg tcgcggtgac ggaaatcgtc
     1201 ttcgcccgcg ccaattattt cgcggtcaac tgcttccagg cattcgagcg cggccgcgac
     1261 atggcgatgc tcggcatcct actctatgcc gcgcgcgtcg ccgcggcgct gatctggagc
     1321 gcgacaacca ccgacccgac gcccctgtca tgggcctggt tctacatgac atcgagcttt
     1381 ctcgccggcg cagccagcct gacgctggtc gtcgtcaagc tcggcgcgcc gcagtggcgc
     1441 gcccgctgga gcgactggaa ggagggcgga ttcttcgcgt tgcagatggg cagcttcgtc
     1501 ggcttccgcg aca
//
LOCUS       DASUCJ010001333         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_228156, whole genome shotgun sequence.
ACCESSION   DASUCJ010001333 DASUCJ010000000
VERSION     DASUCJ010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228156"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFU90_15545"
     CDS             complement(<1..731)
                     /locus_tag="VFU90_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481259.1"
                     /translation="MPNSRLLLAVPALLLAAGCASPQPASNSLPSAPSALSSDAGAGV
                     RTLLYAGGPGNNAVDAYPAGADNPKPLREIVNGLSAPTGIAVDSSGNVYVCNNSGFGA
                     RPNGKGNYWTVTVYKRGQKTPFESYTDGVWSPVDVAVAADGTVYIANYSSAVTVYPPG
                     SLQHSLTLTAPTGQAPLGIAFDAAGKIYVSYVLPSGGGSIYKYAPGKIKGTNLGIAFS
                     GASPHGLAFDRQGNLVVAVSNAPNPG"
     gene            875..1459
                     /locus_tag="VFU90_15550"
     CDS             875..1459
                     /locus_tag="VFU90_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481260.1"
                     /translation="MRLAAAATLTIAAFALGGPLAAAPASPAASLIAPTTRDGHKDFD
                     FLFGTWHTHYLILRKRLSHNHDWYACNGTSVIRPFWGGSGNLEDGDLHCPDRYIGGMT
                     LRMYNAGTHQWSLWWGTRTLGLVPPPQVGHFDAKGTGEFYADDTYAGKPIVVRFKWSL
                     RSGDHPRFEQAFSPDGGKTWETNWTTDYTRAGRD"
     gene            complement(1456..>1929)
                     /locus_tag="VFU90_15555"
     CDS             complement(1456..>1929)
                     /locus_tag="VFU90_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481261.1"
                     /translation="GNLFVEGFSWYGPAICELPLDGSSLETVTFSGATLSTPGGVMWD
                     GKHLTFSDPHTGADRKETTLYRAAESASGDLTVVGATLLKDSCNHDYTWIDAPFIVGA
                     RNTPVNDRRGKVVVGPNNECYPEPIDFWPYAGGQSPTQAWMEDYVPGGVVVSIGS"
BASE COUNT          324 a          601 c          664 g          340 t
ORIGIN      
        1 ccggggttcg gcgcgttgct caccgccacg acgagatttc cctgacggtc gaacgcgagg
       61 ccgtgcgggc tcgcgcccga gaacgcgatg ccgaggttcg tgcctttgat tttcccggga
      121 gcatacttgt agatgctgcc gccgccgctg ggaagcacgt agctcacgta aatctttccg
      181 gctgcgtcga acgcgattcc gagcggcgct tggccggtgg gtgcggtgag ggtgagcgaa
      241 tgctgaagac tgcccggcgg gtacacggtg acggcgctgc tgtagtttgc gatgtagacg
      301 gttccgtcgg ccgcgaccgc cacgtcgacg gggctccaaa cgccgtcggt gtaggattcg
      361 aacggcgtct tttggccgcg cttgtaaacg gtcacggtcc aatagttgcc tttgccgttc
      421 ggacgcgcgc cgaaaccgct gttgttgcac acgtacacgt tgccggacga atccacggcg
      481 atgccggtcg gcgcgctaag gccgttgacg atctcgcgca gcggtttggg gttgtcggcg
      541 ccggcggggt aggcgtcgac cgcgttattg cccggaccgc ccgcgtagag cagggtgcga
      601 acgcccgcgc cggcatcgct ggaaagcgcg gacggagctg atggaaggga gttactcgcg
      661 ggctgcggcg aggcgcatcc ggcggccagc agcaacgcgg gtacggcgag aagaaggcgg
      721 gagttcggca tagagcgcgc gcttcggtag ccttcacggg cgtcctccag ggattcgaag
      781 ggcaacgctc gggttgcgtc acaacggcgg cgggcggcga gtttgaactc gatggcgccc
      841 gcactcggcg cggcgtcgca cacggagggt ggggatgagg ctcgcggctg ctgcgacgtt
      901 gacgatcgcg gcgtttgctc tcggaggtcc gctggcggcg gcgccggcgt caccggcggc
      961 ttcgctgatc gcaccgacga cgcgcgacgg gcacaaagac ttcgacttct tgttcggcac
     1021 gtggcacacg cactacctga ttctgcgcaa gcggctcagc cacaatcacg actggtacgc
     1081 ctgcaacggc acgtcggtga tccggccgtt ctggggcgga agcggcaacc tcgaggacgg
     1141 cgatctgcac tgtcccgacc gctacatcgg cgggatgacg ctgcgcatgt acaacgccgg
     1201 cacgcatcag tggtcgctgt ggtggggaac ccgcacgctc gggctcgtgc cgccgccgca
     1261 ggtcgggcac ttcgacgcaa agggcaccgg cgagttctac gccgacgaca cctatgcggg
     1321 aaagccgatc gtcgttcgct tcaagtggtc gctgcgcagc ggcgatcatc cccgtttcga
     1381 gcaagccttc tcacccgacg gcggcaagac gtgggaaacg aactggacga ccgactacac
     1441 ccgcgccggc cgtgattaac ttccgatgct gacgacgacg ccgcccggca cgtaatcttc
     1501 catccaagcc tgcgttgggc tttgaccgcc ggcatacggc caaaaatcga tcggttcggg
     1561 atagcactcg ttgttcggtc cgacgacgac cttgcctcgg cggtcgttga ccggcgtgtt
     1621 cctcgcgccg acgatgaagg gcgcgtcgat ccaggtgtag tcgtggttgc agctatcctt
     1681 cagcaacgtc gcgccgacga cggtaagatc tcccgaggcc gactcggccg cgcggtagag
     1741 ggtcgtttct tttcggtccg caccggtgtg cggatcgcta aacgtcaaat gcttgccgtc
     1801 ccacatgacg ccgccgggcg tgctcagcgt cgcacccgag aacgtaacgg tttcgagcga
     1861 actgccatcg agcggcagtt cgcaaatcgc cgggccgtac caagagaatc cttcgacgaa
     1921 aaggttgcc
//
LOCUS       DASUCJ010001334         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1129923, whole genome shotgun sequence.
ACCESSION   DASUCJ010001334 DASUCJ010000000
VERSION     DASUCJ010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129923"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFU90_15560"
     CDS             complement(<1..1020)
                     /locus_tag="VFU90_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5481262.1"
                     /translation="MAIEGTVDAAAIYLHDVQIGGASHDAFFVTTTYGKTIAVDAAAG
                     TVLWTYTPPNYSSWAGSRQITNATPVADPDRQSIYAASPDGHIQKLAVSDGHAMWSTA
                     ITKLAHREKIASSLNYFRGRVIAATAGYIGDAPPYQGHVSILDAANGALLHTWNSLCS
                     DSTSMIEPSTCAESGSAIWGRTGAVIDTATGDIYVATGNGKWDGHTFWGDATIRLDST
                     ATRMLGNYTPENTQSLDETDTDVGSTSPALLGGGFVAQGGKDGTIKLLDTTQMQGTTP
                     HRGGERQSVSTPTGARLFTALTVMHTPNATWLFAADNGGTAAWTFRDEKLVPAWKNRN
                     GGTTPV"
BASE COUNT          151 a          346 c          350 g          195 t
ORIGIN      
        1 caccggcgtc gtcccgccgt tccggttctt ccaggccggc acgagcttct cgtcgcggaa
       61 cgtccatgcg gccgtgccgc cattgtcggc ggcgaagagc cacgtcgcgt taggcgtgtg
      121 catcaccgtc agcgcggtaa agaggcgcgc gcccgtcggc gtggatacgc tctggcgctc
      181 accgccccgg tgcggcgtgg tgccctgcat ctgcgtcgtg tcgagcagct tgatcgtgcc
      241 gtccttgccg ccctgcgcca cgaagccgcc gcctaacagg gccggcgacg tcgagccgac
      301 gtcggtgtcg gtctcgtcca gcgattgcgt gttctcgggc gtgtagttgc ccaacatcct
      361 ggtcgccgtc gagtcgagcc ggatcgtcgc gtcgccccag aacgtgtggc cgtcccactt
      421 gccgttgccg gtcgcgacgt agatgtcgcc ggtggcggta tcgatcaccg cgccggtgcg
      481 tccccagatt gccgaaccgc tctccgcaca cgtactcggc tcgatcatcg aggtggagtc
      541 gctgcacagc gagttccagg tgtgcaacaa cgcgccgttc gccgcatcga gaatcgatac
      601 gtgtccctga tagggaggcg cgtcgccgat atacccggcg gtggccgcga tcacgcgtcc
      661 gcgaaaataa ttgagcgacg atgcaatctt ctcgcggtgc gcgagcttgg tgatcgcggt
      721 gctccacatc gcatggccgt cggacaccgc gagcttctga atgtgtccgt cgggcgacgc
      781 ggcgtaaatg gactgccggt ctgggtcggc gaccggcgtg gcattcgtga tctgccgcga
      841 gccggcccac gatgaatagt taggcggtgt ataggtccag agcaccgtgc ccgcggccgc
      901 atcgaccgcg atcgtcttgc cgtatgtcgt ggtgacgaag aaggcatcgt gcgatgcccc
      961 gccgatctgg acgtcgtgca ggtagatggc cgccgcatcg acggtgccct cgatcgccac
     1021 gcgctccttg gcgagcgacg cc
//
LOCUS       DASUCJ010001335         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_548711, whole genome shotgun sequence.
ACCESSION   DASUCJ010001335 DASUCJ010000000
VERSION     DASUCJ010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548711"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFU90_15565"
     CDS             complement(<1..211)
                     /locus_tag="VFU90_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5481263.1"
                     /translation="MKGVILAGGLGTRLRPLTKVTNKHLLPVYDRPMIYYPIETLCRA
                     GIRDIMIVTGGSSAGDFLRLLGNGSD"
     gene            325..1134
                     /gene="cysD"
                     /locus_tag="VFU90_15570"
     CDS             325..1134
                     /gene="cysD"
                     /locus_tag="VFU90_15570"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU5481264.1"
                     /translation="MPTATDSDLDALESRTIYILREAFSTVSPIAMLWSIGKDSTALL
                     WMVRKAFLGDVPFPMVLLDTGMEFPEVYAFRDRLVREWRLPVVNEMCPPESTVDPTLP
                     PAARHAARKSAGLRALLQREGYRGIVLGIRRDEQAIRAKERIFSPRNADGSWDPRTQP
                     PELWDYYPYEVADGAHVRIHPLLHWTELDIWRYTRREGIPFVPLYLAIDGKRYRSLGE
                     SNITVPIESTASTLDDVIAELEKTREPERAGRTMDNESEDAFERLRTTGYM"
     gene            1131..>2036
                     /locus_tag="VFU90_15575"
     CDS             1131..>2036
                     /locus_tag="VFU90_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5481265.1"
                     /translation="MRQVRIVMVGDVDAGKSTILGRMLVDLGQVTPQKLAELENQSTR
                     RGVPIEYSFLLDAFQLERDQAITLDISRIWLRMPERDYVFVDAPGHRELIRNLLTGAS
                     EVDAAILVVAEDEGITLQTRRQALFLQWFGFKEILVAINKLDLAEDAETAFAQRKAEV
                     RAFLEQLGIVPIDIIPIAARDGDNIVEGSRRWPWWKGETMLESLEKLRPHTPQASGPL
                     RFVVQDVYRRGANRIVAGRIDAGTLRSGERIVFWPLQTDAVVTAIHRWPDDVSDARAG
                     DAVAISLDERVFVDRGAVASYPGDGP"
BASE COUNT          371 a          640 c          671 g          354 t
ORIGIN      
        1 agtcgcttcc gttgcccagc aatcggagaa agtctccggc ggagctgccg cccgtgacga
       61 tcataatgtc gcggatgcca gcgcggcaga gcgtctcgat cggatagtaa atcatcggcc
      121 gatcgtagac cggcagcaga tgtttgttgg tgaccttcgt aagcggccgc aatcgcgtgc
      181 ccagtccgcc cgcgaggatg acgcctttca acgtgagtcc tccacagccg cttcgttcca
      241 gtggcagggg gctatttact gcgccgggag tagggggccc taggtggtag gaaagtgggc
      301 cgtgcgggtc gatactagag tccgatgccg acggccaccg attccgatct cgacgcgctc
      361 gagtcgcgta cgatctacat tctgcgcgaa gctttttcga cggtttcgcc gatcgcgatg
      421 ctgtggtcga tcggcaaaga ttcgaccgcg ctgctgtgga tggtgcgcaa agcgtttctc
      481 ggcgacgttc cgtttccgat ggtgctgctc gacaccggaa tggaatttcc ggaagtctat
      541 gcgttccgcg accggctggt gcgcgaatgg cgcctgccgg tggtcaacga gatgtgcccg
      601 ccggaaagca ccgtcgatcc gacgctgccg ccggccgcgc gtcacgccgc ccgcaagagc
      661 gcgggcctgc gcgcgctgct gcagcgcgaa gggtaccgcg gcatcgtctt gggcatccgg
      721 cgcgacgagc aagcgatccg cgcgaaagag cgtattttta gcccgcgcaa cgccgacgga
      781 tcgtgggatc cgcggacgca gccgccggag ctgtgggact actatccgta cgaagtggcc
      841 gacggcgcgc acgttcgcat ccatcccttg ctccattgga ccgagctcga catctggcgc
      901 tacacccgtc gcgaagggat tccgttcgtg ccgctctacc tcgcgatcga cggcaaacgt
      961 taccgttcgc tcggggagag caacatcacg gtgccgatcg agagcacggc gtcgacgctc
     1021 gacgacgtga tcgcagagct ggaaaagacg cgcgagccgg aacgggccgg ccgcacgatg
     1081 gataacgaaa gcgaagacgc cttcgaaaga ttgcgcacga cgggatacat gtgagacaag
     1141 tcaggatcgt catggtcggg gacgtcgacg ccggaaagtc gacgatcctc ggcaggatgc
     1201 tggtcgacct cggccaggtc acgccgcaaa aactcgccga gctcgaaaat cagagcacgc
     1261 gccggggcgt gccgatcgag tactcgttct tgctcgacgc cttccagctc gagcgcgatc
     1321 aggcaatcac gctcgacatc tcgcgcatct ggctgcgcat gcccgaacgc gattacgtat
     1381 tcgtcgacgc gccgggccat cgggagctga ttcgaaacct gcttaccggc gcgtccgaag
     1441 tcgacgcggc gattctggta gtcgccgaag acgaaggcat cacgctgcag acgcgccgcc
     1501 aggcgctctt cctgcagtgg ttcggcttca aagaaatcct cgtcgcgatc aacaagctcg
     1561 atctggcgga ggacgccgaa accgcgttcg cccagcggaa ggcggaagtg cgcgcgttcc
     1621 tcgagcaact cgggatcgta ccgatcgaca tcatcccgat cgcggcgcgc gacggcgaca
     1681 acatcgtcga aggaagccgc cgttggccgt ggtggaaggg cgagaccatg ctcgaatcgc
     1741 tcgagaagtt gcgtccgcat actccgcagg ccagcggtcc gctgcgcttc gtcgttcaag
     1801 acgtgtatcg ccggggggcg aatcggatcg tggcgggccg catcgacgcc ggcactttgc
     1861 gctcgggcga gcggatcgtc ttttggccgc tgcaaaccga cgcggtcgtt accgcgattc
     1921 accgttggcc cgacgacgtc tccgacgcgc gcgccggcga cgcggtggcg atctcgctgg
     1981 acgagcgcgt tttcgtcgat cgcggcgcgg tcgcgagcta tcccggcgac ggcccc
//
LOCUS       DASUCJ010001336         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1390014, whole genome shotgun sequence.
ACCESSION   DASUCJ010001336 DASUCJ010000000
VERSION     DASUCJ010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390014"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1161
                     /locus_tag="VFU90_15580"
     CDS             16..>1161
                     /locus_tag="VFU90_15580"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEU5481266.1"
                     /translation="MRVLQALRWDDAVEANFLKGRPRENPRIDSAYYDKVDLGFDVRA
                     KCEEFEAIARDIEQSLGANDPIAAIMLATALEYRDVVQMLAARGTPAFYTASRKLYGS
                     PKDKFPDGRLSVRDLGRLLYGLLTKVDEWGGPGFGGSPAYPRTIDAAEAARQLGERLA
                     AFFGDAHVRVEADNGILADAAAGSDYVKVRTGAKFSQRDIEILEVHEGWVHVATSLNG
                     QQQPVAKWLAKGPPRTAAVQEGLAALVEMFTFRTHPRRARRLNDRILAVDKAEDGADF
                     LDVFEWFRTEGYDEQECFNNTRRVFRGGVVTGGAPFTKDACYCKGIVLNYAFIRSAIQ
                     HNRLDLIPMLFLGKVAHEDVPVLARRISDGVVKPPRFLPPMFRDLNGL"
BASE COUNT          197 a          382 c          397 g          185 t
ORIGIN      
        1 gaggcgcagc gtccgatgcg cgtgcttcag gctttgcggt gggacgacgc ggtcgaagcg
       61 aactttctga aaggccggcc gcgcgagaat ccgcgaatcg acagcgcgta ctacgacaag
      121 gtcgatctcg ggttcgacgt ccgcgcgaag tgcgaagagt tcgaagcgat cgcgcgcgac
      181 atcgaacaga gcctcggcgc gaacgacccg atcgccgcga tcatgctcgc gacggcgctc
      241 gaataccgcg acgtcgtgca gatgctcgcg gcgcgcggga ctcccgcgtt ctacacggcg
      301 tcgcgcaagc tgtacggatc cccgaaggac aagtttccgg acgggcgcct cagcgtgcgc
      361 gatctcggcc ggctgctcta cggtcttttg accaaggtcg acgaatgggg cgggccgggg
      421 ttcggcggtt cgccggcgta tccgcgcacc atcgacgccg cggaggcggc gcgccagctc
      481 ggtgagaggc tcgcggcgtt cttcggcgac gcgcacgtgc gcgtcgaggc ggacaacggg
      541 atcctggccg acgccgccgc cggcagcgac tacgtcaaag tgcggacggg cgcgaagttc
      601 agccagcgtg acatcgagat tctcgaagtg cacgagggct gggtgcacgt ggcgacgtcg
      661 ctcaacggcc agcagcagcc ggtggcgaaa tggcttgcga aaggcccccc gcgcaccgcc
      721 gcggtgcagg agggcctggc cgcgctcgtc gagatgttca cgttccgcac ccacccccgg
      781 cgggcgaggc ggctcaacga tcgcatcctc gccgtcgaca aagccgaaga cggggccgac
      841 tttctcgacg tgttcgagtg gtttcgcacc gagggctacg acgaacagga gtgcttcaac
      901 aatacccgcc gcgtctttcg cggcggcgtc gtgacggggg gcgccccctt cacgaaggac
      961 gcctgttact gcaaaggcat cgtgctcaac tacgcgttca ttcggagcgc gattcagcac
     1021 aaccggctcg atctgatccc catgctcttc ttgggcaagg tcgcgcacga agacgtgcct
     1081 gtcctcgcgc gccgcatctc ggacggcgtg gtgaagccgc cgcgcttctt gcctccgatg
     1141 ttccgcgacc tgaacggcct t
//
LOCUS       DASUCJ010001337         1339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_29471, whole genome shotgun sequence.
ACCESSION   DASUCJ010001337 DASUCJ010000000
VERSION     DASUCJ010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29471"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..>1339)
                     /locus_tag="VFU90_15585"
     CDS             complement(174..>1339)
                     /locus_tag="VFU90_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481267.1"
                     /translation="GGPNVVLVSEAYGETDSRNDPTVRANQFRHTSGVIDFSEAYASG
                     KLGPIVLSVGRGREAWIGDGTESLVLSANGPALDKITLTAQWSKFEFKALVAELNDVV
                     LSPATDSLPDSVGTQRYHRMMVAHALTWRPSHKVELTLGETALIPHQGGGIALNFVNP
                     VMVLQVTQNDNSGDVSRAGDVNLTAFGSVRANFDRFALQGDLLIDDIQIDAQDRKNFP
                     DLFGWNVRGTYALSLPVPANLGLQYRRVSAFTYLESFYTATWQQYDQPVGSELGPDAD
                     MARAFADVWPTGKFHVSAGISKWRRGDQRISQRPPPTRFGHAGAPFPFASGDTPDVQS
                     AWMGDASVQWLDPIVPLTLVVQGARVTNVNNVATPTGTYGRVQLVATYRFRYP"
BASE COUNT          229 a          467 c          423 g          220 t
ORIGIN      
        1 tcgccatgtg cgccggcgtc cagaacgtgt cgcggccaat cgtgctgtgc cacgagatgt
       61 cccacatgac gccgacgatc acgcacagcg atgccagcag gaccgccgac aggtaccacg
      121 gcacgccgat cgtcccttcc ccgcccgctc ggcgatgcgg ctccatcgcc gactcaggga
      181 tagcggaagc ggtatgtcgc cacgagttgc acgcgtccgt acgtcccggt cggcgtcgcg
      241 acgttgttca cgttggtcac gcgcgcgccc tgcaccacga gcgtgagcgg cacgatcggg
      301 tcgagccact gcacgctcgc gtcgcccatc cacgcgctct gcacgtcggg cgtgtcgccg
      361 ctcgcaaacg gaaacggcgc gccggcgtgg ccgaagcgag tcggcggcgg acgctggctg
      421 atccgctggt cgccgcgacg ccacttggag atgcccgcgg acacgtgaaa cttgcctgtg
      481 ggccacacgt cagcaaaggc ccgcgccatg tcggcatcgg ggcctaactc ggaccccacg
      541 ggctgatcgt actgctgcca cgtcgcggtg tagaacgact cgagatacgt gaacgcgctc
      601 acgcgccggt attgcagccc cagattcgcc ggcacgggaa gcgacaatgc gtaggtcccg
      661 cgcacgttcc agccgaacaa gtcggggaag ttcttccgat cctgcgcgtc gatctgaatg
      721 tcgtcgatga gcagatcgcc ctgcagcgcg aaccgatcga aattggcgcg caccgatccg
      781 aacgccgtca ggttcacgtc gccggcccgg ctgacgtcgc ccgaattgtc gttctgcgtg
      841 acctgcaaca ccatcaccgg attcacgaag ttgagcgcga tcccaccgcc ctggtgcgga
      901 atgagcgccg tctcgccgag cgtcagctcg accttgtgcg acggccgcca agtgagcgcg
      961 tgcgccacca tcatccgatg gtaccgctgc gtcccgacgc tgtccggcaa actgtcggtg
     1021 gccggcgaca gcaccacgtc gttgagctcg gcgacgaggg ctttgaactc gaatttcgac
     1081 cactgtgccg tgagcgtgat cttgtccaac gccggaccgt tcgccgagag cacgagcgac
     1141 tcggtgccat cgccgatcca cgcttcgcgg ccgcgcccaa cggagagcac gatcgggccc
     1201 aacttgcccg atgcataggc ttcgctgaaa tcgatgacgc cggacgtgtg ccggaattgg
     1261 ttggcgcgca ccgtcggatc gttgcggctg tcggtctcgc cgtacgcctc actgaccagc
     1321 accacgttcg ggccgccat
//
LOCUS       DASUCJ010001338         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_686821, whole genome shotgun sequence.
ACCESSION   DASUCJ010001338 DASUCJ010000000
VERSION     DASUCJ010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686821"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFU90_15590"
     CDS             complement(<1..581)
                     /locus_tag="VFU90_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5481268.1"
                     /translation="MRIQAPDELSMSEISDDAKRNREGWTKANADYTDAHASRSWAAA
                     EITWGVFGVPEASIGTLGEVADLDVVELGCGTAYVSAWLARRGARPVGVDVTPAQLAT
                     ARRCQREFGIEFPLIEASAESVPLPSESFDLAVSEYGASIWCDPHRWIPEAHRLLRPG
                     GRLWFLRNSTLAILCASEEGPPTTTLQRPQRGLG"
     gene            711..>1012
                     /locus_tag="VFU90_15595"
     CDS             711..>1012
                     /locus_tag="VFU90_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481269.1"
                     /translation="MTDLILKGGRVFDPGKGLDVIADVAFSAGKVTEIRPAIAPERRT
                     STIDVAGKIVVPGMIDLHTHVYWGGTSLGVEAEPVMRRSGTTTFVDAGSAGPGNFAG"
BASE COUNT          175 a          330 c          331 g          176 t
ORIGIN      
        1 cccagtccgc gctgcgggcg ctggagagtg gtggtcggtg gcccttcctc cgacgcgcac
       61 aggatcgcaa gcgtgctatt gcggagaaac cacaagcggc cgcccggccg cagcaatcga
      121 tgggcttccg ggatccaccg atgcggatcg caccagatgc tcgcgccgta ctccgacacc
      181 gccaggtcga agctctcgga cggcagcgga acgctctcgg cgctcgcctc gatcagcggg
      241 aactcgattc cgaattcgcg ctggcagcgg cgcgcagtcg cgagttgcgc cggcgtcacg
      301 tcgacgccca ccggacgcgc accgcgtctt gccagccagg ccgaaacgta cgcggtgccg
      361 cagccgagct cgacgacgtc gagatcggcg acttcgccga gtgtgcctat agaagcttcc
      421 ggaacgccga agacgcccca ggtgatctcg gcggccgccc agctccggct cgcgtgcgcg
      481 tccgtgtagt ccgcgttcgc cttcgtccag ccttcgcgat tgcgcttcgc gtcgtcggat
      541 atttcgctca tcgacagctc atcgggggcc tgaatgcgca catcatcgac cgttcaaatt
      601 gactcgcaga atatgtcaaa cgtaccattt cttcgaacaa tccattgagc gctcgggctc
      661 gccgtgaccg atcgtcgtgt tgcgggggtc tgtaacgaca agggcatcgg atgactgatc
      721 tgattctgaa gggaggccgg gtattcgacc ccggcaaggg actcgacgtc atcgccgacg
      781 tcgccttctc cgccggcaag gtcacggaga ttcggccggc aatcgcgccg gaacggcgca
      841 cttcgacgat tgatgtcgcg ggcaagatcg tggtgccggg aatgatcgat ctccacacgc
      901 acgtctactg gggcggaacg tcgctggggg tcgaggccga accggtgatg cggcgtagcg
      961 gaacgacgac cttcgtcgat gcgggcagcg ccggtcccgg gaatttcgcc gg
//
LOCUS       DASUCJ010001339         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_809111, whole genome shotgun sequence.
ACCESSION   DASUCJ010001339 DASUCJ010000000
VERSION     DASUCJ010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809111"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1616
                     /locus_tag="VFU90_15600"
     CDS             84..1616
                     /locus_tag="VFU90_15600"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5481270.1"
                     /translation="MRAHWQVAFATAAVVLCVTSVHSQQPSLPGYTAAAAQRERALEA
                     STTARPDSAQARVDSRTLSSDVHIAGTPAQARTRDFVVDAMKRWGLETNVRAYDVWMP
                     FTTNMEAWRVAPDTLPLRLAEGPVPEDTTSWTVKEPVIANGYSGTGDVSAPVVYVNYG
                     LIEDYAQLDSLGVSVKGKIAIARYGRSYRGIKAREAEKHGAIGLIIYSDPADDGFTRG
                     DVYPLGPMRNPNGVQRGSVLNPDGDPTTPGYASKPGVHRVPLDSVEISHIPVIPMSYG
                     NAAQLLEGLRGKEIPQSWQGGLPFRYHAGPGPVSAHLIVRSDTATAAIKTIWDTFGVV
                     RGSEFPDEIVMIGGHRDAWGPGAADNVSGTVSVMEAARAVAEAVRAGYKPKRTIVFAT
                     WDAEEWGLLGSTEYVEDDSLRLLRGAVAYFNQDVAAQGPAFGGGGSPSIRSTLRDIVS
                     HIPDPSGAGTVYDVWRKRANVPDSAQPRMGDPGGGSDFAGFYNHCGIPIAEWGFGGAG
                     GV"
BASE COUNT          291 a          541 c          591 g          259 t
ORIGIN      
        1 gccggcaacg tttcgcgcac tgcctgcgta gccgggtcgg atgcatcgat cgcgcgagct
       61 catactcact gaaccccacc atcatgcgtg ctcattggca ggtcgcgttc gcgacagcgg
      121 ctgtcgtcct gtgtgtcacc agtgtccaca gccagcagcc atcgctgccg ggctacacgg
      181 ccgccgcggc gcagcgcgag cgcgcgctcg aggcgagtac gacggcccga ccggatagcg
      241 cgcaggcgcg cgtcgactcg cgcacgctgt cgagcgacgt tcacatcgcg ggcacgccgg
      301 cgcaagcacg cacgcgcgat ttcgtggtcg acgcgatgaa gcgctgggga ctcgagacca
      361 acgtgcgtgc gtacgacgtc tggatgccgt tcaccacgaa catggaagcg tggcgcgtag
      421 ccccggacac gctgccgctt cggttggccg agggaccggt gccggaggac accacatcgt
      481 ggacggtgaa ggagcccgtg atcgccaacg ggtacagcgg caccggcgac gtctcggctc
      541 ccgtcgtgta cgtgaactac ggactcatcg aagactacgc gcaactcgat tcgttaggcg
      601 tgtcggtgaa ggggaagatc gcgatcgcgc gctacgggcg gtcgtatcgc ggcatcaaag
      661 cgcgcgaagc cgagaagcac ggcgcgattg ggctgatcat ctacagcgat ccggcggacg
      721 acgggttcac gcgcggcgac gtgtatccgt tagggccgat gcgcaacccg aacggcgtgc
      781 agcgcggcag cgtgctcaat cccgacggcg atcccacgac gccgggttac gcgagcaagc
      841 cgggcgtgca tcgcgtgccg ctcgactcgg tcgagatctc acacattccc gtgattccga
      901 tgtcgtacgg caacgccgca cagctgctcg aaggtctgcg cggcaaggag attccgcaga
      961 gctggcaggg cggcttgccg ttccgctacc acgccggacc cggaccggtg tccgcgcatc
     1021 tcatcgtgcg cagcgacacc gcgacggcgg cgatcaagac gatctgggat acgttcggcg
     1081 tcgtgcgcgg gagcgaattc ccggatgaga tcgtgatgat cggcgggcat cgcgacgcgt
     1141 ggggtccggg cgcggccgac aacgtgagcg gaaccgtgag cgtgatggaa gcggcgcgcg
     1201 cagtggccga agcagtgcgc gcgggctaca agccgaagcg cacgatcgtg ttcgccacgt
     1261 gggacgccga ggaatggggt ctgttaggct cgaccgaata cgtcgaggac gactcgctca
     1321 ggctgctccg cggcgcggtg gcgtatttca atcaggacgt cgcggcgcag gggccggcct
     1381 tcggcggcgg cggctcgccg tccattcgct ccacgttgcg cgacatcgtg agccacatcc
     1441 ccgatccgag cggcgccggc accgtctacg atgtgtggcg caagcgggct aacgtgccgg
     1501 acagcgcgca gccgcgcatg ggcgatccgg gcggcggcag cgacttcgct ggattctaca
     1561 atcactgcgg gattcccatc gccgaatggg gattcggcgg cgcgggcggc gtctagcact
     1621 cgctctacga ttcgtaccac tggctgacga agttcggcga tccgacagtc ggataccatg
     1681 cc
//
LOCUS       DASUCJ010001340         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_640866, whole genome shotgun sequence.
ACCESSION   DASUCJ010001340 DASUCJ010000000
VERSION     DASUCJ010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640866"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..659
                     /locus_tag="VFU90_15605"
     CDS             231..659
                     /locus_tag="VFU90_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481271.1"
                     /translation="MGKAIHGRWAAAAAAAAMLATAGVAQAIPAPRVVTLGDATYVSG
                     GVGKLEQNAMRGMESHYPIAMTFAKHNQGANEFVAGVRLRVTDRSGKSVVDLSDGGPM
                     LLLKVPDGKYTVRAEYEGHVKTQSVDVTRGHHANLDFLWS"
     gene            710..>1550
                     /gene="argG"
                     /locus_tag="VFU90_15610"
     CDS             710..>1550
                     /gene="argG"
                     /locus_tag="VFU90_15610"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5481272.1"
                     /translation="MSRILQSLPTGKKVGIAFSGGLDTSAALHWMRANGAIPYAYTAH
                     LGQPDEHDYDDIPRRALRYGAEAARLVDCRAALVGEGIAALQAGAFHVSTAGATYFNT
                     TPLGRAVTGTMLVVAMLEDDVHIWGDGSTYKGNDIERFYRYGLLANPNLSIYKPWLDQ
                     RFIDELGGRREMSEYMQRSGLDYRMSAEKAYSTDSNLLGATHEAKDLEYLDAGIDIVV
                     PIMGVAFWRVDVPITPERVVVAFDAGHPVALNGKRYADAVDLMTEANAIGGRHGLGMS
                     DQIE"
BASE COUNT          276 a          502 c          522 g          250 t
ORIGIN      
        1 tggtcgacgg cgttgcgaac ttcgacctgg ttcacttcgg acacgatgtc gaacgacggc
       61 atggagtctc ctcgcggaat tcgaaggcgc agaccgggcg cctattgtag gccgctcacc
      121 gacacgcgcc ccgaggtagc ggaggccccg acccgccgcg gccggcacga cggatcttcg
      181 caccgtcgag tgcggtcata tccagtgttt ggcaaccagg cggaggcaat atgggcaaag
      241 caattcatgg acggtgggcg gccgcggcag ccgcggcggc gatgcttgcg acggcaggtg
      301 tggcgcaggc gattccggca cccagggtgg tcacgctcgg cgacgcgacg tacgtgagcg
      361 gcggcgtcgg gaagctcgaa cagaatgcaa tgcgcgggat ggagtcgcac tatccgatcg
      421 cgatgacgtt cgcgaagcac aaccagggtg cgaacgagtt cgtcgccggg gtcaggcttc
      481 gcgtcaccga tcgcagcggc aagtcggtcg tcgatctcag cgacggtggt ccgatgctgc
      541 tgcttaaggt tccggacggc aagtacacgg ttcgcgccga atacgaaggt cacgtcaaga
      601 cgcaatcggt cgacgtgacg cgcggccatc acgcgaacct tgattttctc tggtcgtaac
      661 gtgggctaaa ctgcggggct ttccggcgtc gcgccgctcg aaagccccca tgtcccgcat
      721 cctgcagagt ctgccgaccg gcaagaaggt cggcatcgcg ttctcgggcg gtctcgatac
      781 cagtgccgcg ctgcattgga tgcgcgccaa tggcgcgatc ccgtacgcct acaccgcgca
      841 tctcggccag cccgacgagc acgactacga cgacatcccg cggcgcgcgc tgcgctacgg
      901 tgcggaggcc gcgcgcctcg tcgattgccg cgctgcgctg gtcggcgaag gcatcgccgc
      961 gctccaggcc ggcgcgttcc acgtgtcgac cgccggcgca acgtacttca atacgacgcc
     1021 gctcgggcgc gccgtgaccg ggacgatgct cgtcgtcgcg atgctcgagg acgacgttca
     1081 catctggggt gacggcagca cctacaaggg taacgacatc gagcgcttct accgctacgg
     1141 cctcctcgcc aacccgaatc tcagtatcta caagccgtgg ctcgaccagc gcttcatcga
     1201 cgagctcggc ggacgccgcg agatgtccga gtacatgcag cggtcgggtc tggactaccg
     1261 gatgagcgcc gagaaggcgt actcgaccga ttcgaacctg ctgggcgcga cgcacgaggc
     1321 gaaggacctc gaatacctcg acgcgggcat cgacatcgtc gtgccgatca tgggtgttgc
     1381 gttctggcgc gtcgatgttc cgatcacgcc cgagcgcgtc gtcgtcgcgt tcgatgccgg
     1441 gcacccggtg gcgctcaacg gcaaacgcta cgccgacgcg gtcgacctga tgaccgaggc
     1501 gaacgcgatc ggcgggcgtc acggcctcgg catgagcgac cagatcgaga
//
LOCUS       DASUCJ010001341         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1405061, whole genome shotgun sequence.
ACCESSION   DASUCJ010001341 DASUCJ010000000
VERSION     DASUCJ010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405061"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..696
                     /locus_tag="VFU90_15615"
     CDS             103..696
                     /locus_tag="VFU90_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481273.1"
                     /translation="MLGALAGLSVWLAAPSARADRYYVVVREDPYEPRSGFNLGFDLE
                     GAAPIATPRFPSGNNLSGGGGFKVRFGDEIRLRRVRVTPEGGYAYDHLFATDDLGNHY
                     DWDTHRLFGGVRVMFGHIVTPGFYVHLGYGWRNTGDPSIPPSADGGLAFDAAFLLDVH
                     ITPRFALGGHIEYVTIDAQPYVPQWLAFGLHGDITFF"
     gene            775..1062
                     /locus_tag="VFU90_15620"
     CDS             775..1062
                     /locus_tag="VFU90_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481274.1"
                     /translation="MTLFEFVIYLVIAGVCGAIARSIAGGTGGGFIVSVLLGFLGAFV
                     GTWIARMVHLPVLVVVDIAGHPFPIVWSIIGGFLLAALAHALVRPRYMYIR"
     gene            1269..>1841
                     /locus_tag="VFU90_15625"
     CDS             1269..>1841
                     /locus_tag="VFU90_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481275.1"
                     /translation="MPLAAPWVSGCTSQDRATITITTGGETDTFTQIPAPTALQVAAV
                     QSDGDGGEKTTILADATLPATSVDLGSLDESASAVITVTGFDSNSHAILSGQSLDLDF
                     SNLPGSNVPVFVQRKGQFARLPNPLTDTRTSPLLAILQGEYLLVTGGDDVDAGATTSL
                     YDFLGLSPTTGPQNIYRAAQSIAIDGAVAWL"
BASE COUNT          262 a          608 c          611 g          360 t
ORIGIN      
        1 tcttcgcgct ttcctcgtcg tctacgcccc tcgcgcgcgc cccgcgcgtg cggcgcctct
       61 tgtgccctcg gctctcgcgg cggccgcgcc gccgtcaccg ggatgctcgg cgccttggcc
      121 gggctctcgg tatggctcgc cgcgccgtcg gcgcgtgcgg accgctatta cgtcgtcgtg
      181 cgggaggatc cgtacgagcc gaggagtgga ttcaacctcg gcttcgatct ggagggggcg
      241 gcgccgattg cgacgccgcg ctttccgagc ggaaataact tgtcgggggg cgggggattc
      301 aaagtccgat tcggcgacga gattcgcttg cgccgcgtgc gagtgacgcc ggagggcggc
      361 tacgcctacg atcatctctt cgcgacggac gacctgggca accactacga ctgggacacg
      421 caccggctct tcggcggcgt ccgggtcatg ttcgggcaca tcgtgacgcc cgggttctac
      481 gtgcacctcg gctacggctg gcgcaacacc ggagacccga gcattccgcc gtcggctgac
      541 ggcgggctcg cgttcgacgc cgcctttctc ctcgacgtgc acatcacgcc gcgcttcgcc
      601 ctaggcggcc atatcgaata cgtgacgatc gacgctcagc cctacgtgcc ccagtggctg
      661 gcattcggtc tgcatggaga cattacgttc ttctaacgac ttcgaagacg gcgccgctcg
      721 agcgacgccc tcgcgcaatg gcgcgataca cggtgcgcag aaggagattt ggtcatgacg
      781 ctattcgaat tcgtcattta cctcgtcatc gcgggagtgt gcggcgccat cgcgcggagc
      841 atcgccggcg ggaccggtgg cgggttcatc gtctcggtgc tgctgggctt tctcggcgcg
      901 ttcgtcggga cgtggatcgc tcgcatggtc cacttgcccg tcctcgtcgt ggtcgacatt
      961 gcggggcatc cgttcccgat cgtctggtcg atcatcggcg gcttcttgct cgccgccctc
     1021 gcccacgcgc tcgtccggcc gcgctacatg tacatccgtt agaccggcgt cgaaacctcg
     1081 aagagccgcg ccgtccgcga aacggctgcc ggctcggggg agggggaaga ctgtgccgat
     1141 gcccatgctc acacgggcag catctgcgcg tcttccccct tcgttttctt tgcgctcgaa
     1201 gacgtgttag ccaagcggcc gtgcgccgct ggttgaatcg gtcttctcgt ttcgaattgt
     1261 gtctgtactt gcctttggcc gcgccttggg tctcgggttg cacgtcgcaa gaccgcgcga
     1321 cgatcacgat cacgacgggc ggcgagacgg acacgttcac ccagatcccg gcgcccacgg
     1381 cgctgcaagt ggcggcggtg cagtccgacg gggacggcgg ggaaaagacg acgattctcg
     1441 cggatgcgac gttgcccgcg acatcggtcg atctcggatc gctcgacgag agcgcgagcg
     1501 ccgtcatcac cgtcacgggg ttcgactcga acagccacgc gatcctctcg gggcagtcgc
     1561 tcgatctcga cttctcgaat ctgccggggt cgaacgtccc ggtgttcgtt cagcgcaaag
     1621 ggcagttcgc gcgtttgccg aacccgctga ccgacacgag gacgtcgcct ctgctcgcga
     1681 ttttgcaggg ggaatacttg ctcgtcacgg gcggggacga cgtggatgcc ggggcgacga
     1741 cttcgctcta cgattttcta gggctgtcgc cgacgacggg gccgcagaac atctatcgcg
     1801 ccgcgcagtc gatcgcgatc gatggcgcgg tcgcgtggct c
//
LOCUS       DASUCJ010001342         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_29515, whole genome shotgun sequence.
ACCESSION   DASUCJ010001342 DASUCJ010000000
VERSION     DASUCJ010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29515"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFU90_15630"
     CDS             <1..756
                     /locus_tag="VFU90_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481276.1"
                     /translation="AAMATALGCNGRMDGAPEGVDAGVADGSADGAGTVIVDASGGGL
                     EAAYPWPCPTQGCTNGVPCADNGCCVNAKCLSPGENCASNLGACENKSCGGCGALNQA
                     CCVGIGTPGGTCAVDAVYLSAWTNGPGCSDPDTVCTDAGTCQACGAEGQLCCFDDSCG
                     PNLACIGTTCTPCGSVGQPCCQSSCNGGSCLNFGSVIECASPESCDGSACTSCGAEGE
                     PCCPGGPGDAGGGCQGALCMSNGLCGPPFVEHP"
     gene            complement(765..>1024)
                     /locus_tag="VFU90_15635"
     CDS             complement(765..>1024)
                     /locus_tag="VFU90_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5481277.1"
                     /translation="EAAKRGGAWFRRGAVQVHVSLDDVDPKQRESRRHICYAVPNLDE
                     ARVLLQREGVAILEDPRPEPGLSRFYVFDPGGNRIEIAAFA"
BASE COUNT          153 a          337 c          375 g          159 t
ORIGIN      
        1 gccgcaatgg cgacggcact cggctgcaac gggcggatgg acggcgcgcc ggaaggggtc
       61 gatgcgggcg tcgccgacgg ctccgcggat ggcgcgggta cggtcatcgt ggacgcctcg
      121 ggaggcggac tcgaagccgc ctatccctgg ccctgcccga cgcaaggctg cacgaacggc
      181 gtcccgtgcg cggacaacgg ctgctgcgtg aacgcgaagt gcctctcccc gggcgagaac
      241 tgcgcgtcga acctcggtgc gtgcgagaac aaaagctgcg gcgggtgcgg cgcgctgaac
      301 caagcgtgct gcgtcgggat cggcactccc ggcggcacct gcgccgtcga cgcggtctac
      361 ctttcggctt ggacgaacgg gccggggtgc agcgatccgg ataccgtctg caccgacgcg
      421 ggcacttgcc aggcatgcgg ggccgaaggt cagctctgct gcttcgacga cagctgcgga
      481 ccgaacctcg cgtgcatcgg aacgacgtgc acgccttgcg gaagcgtcgg ccagccgtgc
      541 tgccagtcgt cttgcaacgg cggctcgtgc ctcaacttcg gcagcgtgat cgagtgcgca
      601 tcgccggagt cgtgcgacgg ctccgcgtgt acgagttgtg gcgccgaggg cgagccttgc
      661 tgtccgggcg gtcctggcga tgcgggcggg ggctgccaag gtgccctctg tatgagcaac
      721 ggattgtgcg gcccgccatt cgtggaacat ccgtagcgac tcgctcaagc gaacgcggcg
      781 atctcgatgc ggttcccgcc ggggtcgaag acgtagaacc gcgacaagcc cggctcgggg
      841 cgcggatcct cgaggatcgc gacgccttcg cgctgcaaga gaacgcgtgc ttcgtcgagg
      901 ttcggcacgg cgtagcaaat atggcggcgg ctctcgcgct gctttgggtc cacatcgtcg
      961 aggctcacgt gcacttgcac cgcgccccgg cggaaccatg cgccgccgcg cttcgccgct
     1021 tcgg
//
LOCUS       DASUCJ010001343         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1190806, whole genome shotgun sequence.
ACCESSION   DASUCJ010001343 DASUCJ010000000
VERSION     DASUCJ010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190806"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU90_15640"
     CDS             <1..198
                     /locus_tag="VFU90_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481278.1"
                     /translation="IFKVKIGMKVTVKNSAFPHLATGRVERISSLVGSRSQLGDVMIR
                     LDKNDPADRLVGMEVEVVIAP"
     gene            232..954
                     /locus_tag="VFU90_15645"
     CDS             232..954
                     /locus_tag="VFU90_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5481279.1"
                     /translation="MSALDSRPPVISPVVTDRAIEVRGVTFSFTREGPPTLIDIDMDV
                     NPGEIVILTGPSGAGKTTLMTLVGALRTIQSGSIRVFHEELAGLSARGQREIRKRIGF
                     IFQDHNLFDALTTFDTLNLAMKLCDPIPPRAEILRRARTLLTSLGIGDYLHSRPHEMS
                     TGQKQRVAIARALIHRPRIILADEPTASLDRDTTNAVLSVLRRRANEDGVTILMVTHD
                     TLLFDQVDRVVTMAEGRILSNR"
     gene            1061..2134
                     /locus_tag="VFU90_15650"
     CDS             1061..2134
                     /locus_tag="VFU90_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5481280.1"
                     /translation="MSQKVRVAIAGVGNCASALIQGVAFYRGAAASDDVPGLMHIELG
                     GYHIRDIEFVAAYDVNHTKVGRDLADAIRAEPNDTASFATVAPLGVKVERGPTLDGIG
                     PSVSSIIRQSDAPVADVAASLKRAKADVLVSYLPVGSQQASEFYAEQALAAGCAFVNC
                     IPVFIASSESWRKRFEAAGLPLIGDDIKSQVGATIVHRVLARLFDERGVHIDRTYQLN
                     FGGNMDFLNMLDAERVVTKKQSKTQSVMSQLDAQMAPRDVHIAPSDHVRWLGDRKWCY
                     IRIEGTGFGNTPLNCELKLEVWDSPNSAGVVIDAVRCAKLALDRGIKGALQGPSAYFM
                     KSPPKQFDDNEARRQVERFIDGS"
BASE COUNT          391 a          681 c          684 g          385 t
ORIGIN      
        1 atcttcaagg tgaagatcgg gatgaaggtc acggtgaaga actcggcctt cccgcatctc
       61 gcgaccgggc gcgtcgagcg catcagctcg ctggtcggca gccgttctca gctcggcgac
      121 gtcatgatcc ggctcgacaa gaacgacccc gccgatcggc tcgtcggcat ggaagtcgag
      181 gtcgtcattg ccccatagca acgcgacaac ggacggaaag gccgcgggat cgtgagcgcg
      241 ctcgacagcc gcccgcccgt gatatcgccg gtcgtgaccg atcgcgcgat cgaggtccgc
      301 ggcgtgacgt tttcgttcac gcgcgaaggc ccgccgacgc tgatcgacat cgacatggac
      361 gtgaaccccg gcgagatcgt cattctcacc ggcccgtcgg gcgccggcaa aaccacgctg
      421 atgacgctgg tcggcgcgct gcgcaccatt cagtccggca gcatccgcgt gttccacgag
      481 gagcttgccg gcctctcggc gcggggtcag cgcgaaatcc gcaaacggat cggcttcatt
      541 tttcaggatc acaatctgtt cgacgcgctg acgacgttcg acacgctcaa tctcgcgatg
      601 aaactgtgtg atccgatccc gccgcgcgcc gaaatcctgc ggcgcgcccg gacgctgctg
      661 acgagcctcg gcatcggcga ctatcttcat tcgcggcctc atgagatgtc gaccggccag
      721 aaacagcgtg tcgcgatcgc ccgggcgctg atccatcggc cgcgcatcat tctcgccgac
      781 gagcccacgg cgtcgctgga ccgcgacacc accaacgccg ttctgtccgt gctgcggcgg
      841 cgggcgaacg aggacggcgt gaccatcctg atggtgacgc atgacacact gctgtttgac
      901 caggttgacc gggtggtgac catggccgag ggccgtatcc tgtcgaatcg gtaatccagc
      961 tgggaaattt gcggccgctg gcgcggtatc gggattgccg ccgcaatctg gctgtcctat
     1021 ggtcggtttt tcgggttgat cgattcaagc aagcgggcag atgagccaaa aagtccgtgt
     1081 agccattgcc ggcgtgggga attgcgcgtc cgccctgatc cagggcgtcg ccttctatcg
     1141 cggtgccgcc gcgtccgacg acgtgccggg cctgatgcat atcgaactgg gcggctatca
     1201 catccgcgac atcgaattcg tcgcggcata tgacgttaac cataccaagg tcggccgcga
     1261 cttggccgac gcgatccgcg ccgaacctaa cgatacggca agcttcgcca cggtcgcgcc
     1321 gctcggcgtg aaggtcgaac gcggccccac gctggatggg atcgggccga gcgtgtcgtc
     1381 gatcatccgc caatcggatg cgccggtggc cgacgtcgcc gcgtcgctca agcgcgccaa
     1441 ggccgatgtt ctggtgtcgt atctcccggt cggttcgcaa caggcgagcg aattttatgc
     1501 cgagcaggcg cttgccgcgg gttgcgcctt cgtcaactgc atcccggtgt tcatcgcgtc
     1561 gtcggagagc tggcgtaagc gcttcgaggc ggcgggtttg ccgctgatcg gcgacgacat
     1621 caaaagccag gtcggcgcca cgatcgtgca tcgtgtgctg gcgcgcctgt tcgacgaacg
     1681 gggcgtccat atcgatcgca cctatcagct caatttcggc ggcaacatgg attttctgaa
     1741 catgctcgac gccgaaaggg tcgtgaccaa gaagcaatcg aagacgcaat cggtaatgag
     1801 ccagctcgac gcgcagatgg cgccgcgcga cgtgcacatc gcgccgagcg accatgtccg
     1861 atggctgggt gatcgcaagt ggtgctacat ccgcatcgag ggcaccggct tcggcaacac
     1921 gccgctcaat tgcgaactga agctcgaagt ctgggattcg ccgaactccg ccggggtcgt
     1981 catcgacgcg gtgcgctgtg ccaagctcgc gctcgaccgc ggcatcaaag gcgcgctgca
     2041 aggtccgtcg gcctatttca tgaagtcgcc gccgaaacaa ttcgacgaca acgaagcgcg
     2101 gcgtcaggtc gagcggttca tcgacggttc ataaaccggc g
//
LOCUS       DASUCJ010001344         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_762973, whole genome shotgun sequence.
ACCESSION   DASUCJ010001344 DASUCJ010000000
VERSION     DASUCJ010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762973"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="asnB"
                     /locus_tag="VFU90_15655"
     CDS             complement(<1..949)
                     /gene="asnB"
                     /locus_tag="VFU90_15655"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5481281.1"
                     /translation="MCGIAGVLDPTGSLSSEALQHTARAMGQALAHRGPDDHGTWVDD
                     RVGIALAHRRLSVIDLSTEGHQPMVSASGRYVITYNGEIYNYAAVRHDLEAIGAAPVW
                     RGHSDTEVMLAAVERWGMADALQRFNGMFAFALWDRQERVLHLARDRMGEKPLYYGVM
                     GRRFAFGSELSAMRAAPSFRGEVDRDALALLLRYNYVPAPWSIYRDVRKLCPGARAEI
                     RVDGAGLPAIHTFAYWSVAEAARRGIAAAGRCDEREAADLVRAELDRSVRLRMVADVP
                     IGAFLSGGIDSTLVTATMQRLSSQRVRTFTIGFDDGEYDE"
     gene            complement(1000..2235)
                     /locus_tag="VFU90_15660"
     CDS             complement(1000..2235)
                     /locus_tag="VFU90_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481282.1"
                     /translation="MVPKAVLLLAGVYVARRFGHEAFARYSLAAVTLTLAGTLVGSTL
                     SAVASKHVPEFARGDAAATGTGFAAVALLALMLAATLGAGIFVAAPALSALFGTSPPI
                     TDLLRVTGFAVAGSIAGGALQGLLTGSTRFRDAAIVNLMAAMAFGATIGPLARIADGA
                     GVLAALAVFYAVASIATMIAVGRPLRADWAAANRAIRSRVSRLWRYFVPMIVATGTVT
                     PVVWLCNLLLARGAHGPIEVARFGAAYNWFAVVSAVPAVLAQVEFVRMARSRATGDVK
                     GLARGYRLFTLQNFAFVAPIVAIGIAAAGPMGDLFRLDGPSTRNCIRVLLASALMASL
                     GNPAGMFLAVIDRIWIASLLNIGWACIVLTLGWILRQSGASGIAVAFLIAYSTHFVVA
                     TTIARRMLARPGSIRPAEV"
BASE COUNT          468 a          795 c          804 g          325 t
ORIGIN      
        1 cctcgtcgta ctcgccgtcg tcgaacccga tcgtgaacgt gcgtacacgc tgagacgaca
       61 gccgctgcat cgtcgcggtc acgagcgtcg aatcgattcc acccgagagg aaggcgccga
      121 tcggcacgtc ggcgaccatc cgcagccgca ccgagcggtc gagttccgca cgcacgaggt
      181 cggcggcttc gcgttcgtcg caccgaccgg ctgcagcgat cccgcgcctg gccgcttcgg
      241 cgacgctcca ataggcaaac gtgtggatgg caggcaggcc ggcgccatcg acgcggatct
      301 cggcgcgcgc acccggacac agcttgcgta cgtcgcggta aatcgaccat ggcgccggta
      361 cgtagttgta gcgaagcagc agtgcgaggg cgtcgcggtc gacctcgccg cggaatgatg
      421 gcgcggcgcg catcgcggag agctcggagc cgaatgcgaa tcgtcgtccc atgacgccgt
      481 agtaaagcgg cttctcgccc atgcggtcgc gcgcgaggtg cagcacccgc tcctgtcgat
      541 cccagagcgc gaacgcgaac atgccgttga agcgctgcaa cgcatcggcc ataccccagc
      601 gctcgaccgc cgcaagcatc acctcggtgt ctgagtgacc gcgccagacc ggtgcggcgc
      661 cgatcgcctc gaggtcgtgc cggaccgcgg cgtagttgta gatttcgccg ttgtaggtga
      721 tgacgtagcg ccccgacgcg gacaccatcg gctggtgtcc ctccgtcgag aggtcgatca
      781 ccgagaggcg gcgatgggcg agcgcaattc cgacacgatc gtcaacccag gtaccatgat
      841 cgtcgggccc gcgatgcgcg agggcttggc ccatcgcgcg cgccgtgtgc tgcaacgcct
      901 cggacgacag cgagccggtc ggatcgagaa caccggcaat gccgcacatc gggggttggg
      961 gacaatcgca gccggaacgg gagagggcga ccgcgaagtc tatacctcgg caggtctgat
     1021 cgagcccggc cgcgcaagca tccgccgtgc gatcgtcgtc gcgacgacga agtgcgtcga
     1081 atacgcgatg agaaacgcca ccgcgatacc ggacgcgccc gactgtctga ggatccaccc
     1141 aagggtcagg acgatgcagg cccatccgat gttgagcaac gaagcgatcc agatccggtc
     1201 gatcaccgcg aggaacatcc ccgcgggatt gccgaggctc gccatcagcg ccgaggcaag
     1261 tagcacgcgg atgcagttcc gcgtcgaagg accgtcgagg cgaaagaggt cacccatcgg
     1321 cccggcggcc gcaatgccga tcgcgacgat cggcgcaacg aatgcgaaat tctggagcgt
     1381 gaagagtcgg tatccgcgcg cgaggccctt gacatctccg gtcgctctcg accgtgccat
     1441 cctcacgaac tcgacctggg ccagcaccgc cggaacggcc gaaaccactg caaaccagtt
     1501 gtacgcggcg ccgaagcgcg cgacttcgat cggcccgtgc gcaccgcggg caagtaacag
     1561 gttgcatagc catacgacgg gagttaccgt tcccgtagcg acgatcatcg gaacgaaata
     1621 gcgccagagt cgggacacgc gcgaccggat cgctcggttg gcggcggccc aatcggctcg
     1681 caggggtcgc ccgaccgcga tcatcgtcgc gatcgatgcg actgcgtaga acacggcgag
     1741 ggccgccagc acgccggcgc cgtccgcgat gcgagcaagc ggcccgatgg tcgccccgaa
     1801 ggccatagcc gccatcaaat tgacgatcgc cgcgtcacgg aaacgcgtgg aaccggtgag
     1861 cagtccctgc aatgcgccgc ctgcgatcga gccggcgaca gcgaagccgg tcacccgaag
     1921 caggtccgtg atcggcggcg aagtcccgaa cagcgcggaa agcgcgggtg ctgcgacaaa
     1981 gatgcccgcg ccgagcgttg ccgcaagcat caatgcgagg agggcgaccg cggcaaatcc
     2041 cgtgcccgtc gccgcagcat cgccacgcgc gaactcgggg acgtgcttgc tcgcgacagc
     2101 agacagggtc gacccgacca gcgtgccggc gagcgtcagg gtgacggcgg caagcgaata
     2161 ccgcgcaaac gcctcgtggc cgaatcgacg cgcgacgtag actccagcga gcagcagcac
     2221 cgctttcggc accacgtagg caatcccgtt ccacgcgact ggaagatagc gcccacgcgc
     2281 gcgagcgaag agcgagcgtc tgcaggcgat cgggtttggg gtcatcggcg cccgtttgcc
     2341 agcacggaga gcaccaggtc gacgagtttc ggggcggtaa tttctagcga at
//
LOCUS       DASUCJ010001345         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1023036, whole genome shotgun sequence.
ACCESSION   DASUCJ010001345 DASUCJ010000000
VERSION     DASUCJ010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023036"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFU90_15665"
     CDS             complement(<1..544)
                     /locus_tag="VFU90_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481283.1"
                     /translation="MIVRLIVALAVVVVGNAVDGVGFGGITSAVAATLGHGSTEIALT
                     SRGDSWWVSTTVIERRSPTGESLASIRSDASGLGVIERFAVSPHDASLWAVTDGHYLV
                     HVENDGVGVSAHPLPPRIASLAIAQNGDVWVALPDAILQFAADGRPLALHPLASNPLG
                     VGDTARAVPRDSLPKRGDAAD"
     gene            complement(571..>1995)
                     /locus_tag="VFU90_15670"
     CDS             complement(571..>1995)
                     /locus_tag="VFU90_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481284.1"
                     /translation="DFPVTTNPYQKGTAYGGSFVAKLNPAGNALVYSTYVTAATVQSL
                     AIDAAGSAYVTGKATSAFITSPGAFQPNSPVPSSETGFVLKLDPTGSTASYATFLGGT
                     SSGQATAIAVDAQGRAHVGGWAAGNFPLARPLQSVLAGGKDGFVSVLDATGSRLVSST
                     VIGGSQNDTVNTLALGTDGAVYIAGETYSADFPVVNGFQPVKAGYRLINAIVGNAFVA
                     KLDLSASRIVWSSFLGGEICKSYCKSLFDGELFAGDAAYGIAVDAEGHAYVTGLARSW
                     TFPLVDSTSPRKQQDNEDSGFVAKVSAAGTTLLFSTFVRTGFSGSMVMSGFPEGAVNA
                     VAIDGAGTAYVAGDSSYSSDVTTSAGALQAVNPGGPTSIIVKFPPPSRRLTIDVQNHD
                     ADAPSPVTMTASVDGPEASGYVIFRSAANILGAAPLDANRATYVTPLSPGIHTLNAAL
                     STPEGNVDAPPMILVIDQPLVCSK"
BASE COUNT          363 a          626 c          658 g          348 t
ORIGIN      
        1 catcggcagc gtcacctcgc ttcggcaacg aatcccgcgg tactgcgcga gccgtatccc
       61 cgacgccgag cggattcgat gcgagcggat gcaacgcgag cggacgaccg tcggcggcga
      121 attgcaggat cgcgtcgggc agcgcaaccc agacgtcgcc gttctgggcg atcgcgagcg
      181 acgcgatccg cggcggaagc gggtgcgcgc tgacgccgac gccgtcgttc tcgacgtgca
      241 cgaggtaatg cccatcggtc accgcccaca ggctcgcgtc gtgcggcgaa actgcgaagc
      301 gctcgatcac gccgaggccg ctcgcatcgc tgcgaatgct cgccagtgat tctccggtcg
      361 gtgagcgccg ttcgatgact gtcgtcgata cccaccacga atccccgcgg ctcgtcagtg
      421 cgatctcggt cgatccatgc ccgagcgtag cggccactgc gctcgtgatg ccgccaaagc
      481 cgacaccatc gaccgcgttt ccgacgacca cgacggccag cgcgacgatc agtcggacga
      541 tcatgatcat tgctccctgc cggttcgcgt tcatttcgag caaacgagag gttgatcgat
      601 gaccaggatc atcggcggtg cgtcgacgtt accttccggc gtcgacagtg ccgcattcag
      661 ggtgtggatg ccgggggaga gcggcgtgac gtacgtcgca cgattcgcat cgagcggcgc
      721 tgctccgaga atgtttgctg cggacctgaa gatcacgtag ccggatgctt ccggaccgtc
      781 cacgcttgcc gtcatcgtca ccggagaggg ggcatccgcg tcgtgattct ggacgtcgat
      841 cgtcaggcgc cgcgacggcg gggggaactt gacgatgatc gaggtcgggc ctcccgggtt
      901 gaccgcctgg agcgcacccg ccgacgtcgt cacatctgag ctgtagctgg agtcaccagc
      961 gacatatgcg gtgccggcgc cgtcgatggc taccgcgttc accgcacctt cgggaaagcc
     1021 gctcatcacc atggagccgg agaagccggt gcgcacgaac gtcgagaaga ggagggtggt
     1081 tccggcagcc gatactttcg cgacgaaccc cgaatcctcg ttgtcctgct gcttgcgcgg
     1141 ggatgtcgaa tcgacgaggg gaaacgtcca cgaacgcgcc agtccggtca cgtaagcatg
     1201 tccctctgca tccactgcga tgccgtacgc ggcgtcgccg gcaaacagtt ctccatcgaa
     1261 aaggctcttg cagtagctct tgcagatctc gccgccgaga aacgacgacc agacgatccg
     1321 ggatgccgaa aggtcgagct tcgctacgaa ggcgtttccg acgattgcat tgatgagccg
     1381 gtaaccggct ttcaccggct ggaatccgtt gacgacggga aaatccgcag agtaggtctc
     1441 gccggcgata tagacggccc catcggtgcc aagtgcgagt gtattgacag tatcgttttg
     1501 agagccgcca atgaccgtgg aggagacgag gcggcttccc gtcgcgtcga gaacgctgac
     1561 gaatccgtcc ttcccgccgg cgagaaccga ctggagtggt cgcgcgagcg gaaaattgcc
     1621 tgccgcccag ccgccgacgt gcgcgcgtcc ctgggcgtcg acggcgattg cggtcgcttg
     1681 gcctgaactg gtgccgccga gaaaagtggc gtaggacgcg gttgacccgg tgggatcgag
     1741 cttcagcaca aatccggttt cgctggaggg gaccggcgag ttcggctgga acgcgccggg
     1801 actggtgatg aaagcgctcg tcgccttgcc cgtgacgtac gcgctgcccg cagcgtcgat
     1861 cgccagcgat tgcaccgtcg ctgcggtgac gtaagtcgag tagacgagcg cattccccgc
     1921 aggattcagc ttggcgacga acgatccgcc gtaagcggtc cccttctggt acgggttcgt
     1981 cgtcaccgga aagtc
//
LOCUS       DASUCJ010001346         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_473081, whole genome shotgun sequence.
ACCESSION   DASUCJ010001346 DASUCJ010000000
VERSION     DASUCJ010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473081"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFU90_15675"
     CDS             complement(<1..743)
                     /locus_tag="VFU90_15675"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU5481285.1"
                     /translation="MDWGRIRFFLGEVLRNFTRNAGMQATAIGTVAITIVLLGLFLFV
                     RGTLSEIGTHLLDQIEISAYLQPGATPSQVTSIRHLLAADPRIASIEFVSKKQGLAEL
                     RDRTKGVIDTSLLTENPLPDKFRIKVRVPEQVPAVAAGVRRLNGIENVVYGQTVVQRL
                     LQLGVVLRRVGIGVIAVFLAVAGIIISNTIRLTVFARRREISIMQLVGASRWMVRGPF
                     VVEGAITGAVAGAVGALVTFGAAMAAVTSG"
     gene            complement(709..1419)
                     /gene="ftsE"
                     /locus_tag="VFU90_15680"
     CDS             complement(709..1419)
                     /gene="ftsE"
                     /locus_tag="VFU90_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5481286.1"
                     /translation="MISLRNVSLVYPNGVRAIDKVTLEIAKGEFVFLVGHSGTGKSSL
                     LRLLYREARPSTGEITVDGIRVDRLRRSRVPALRRHLGVVFQDFKLLVDKTVWENVAF
                     AMQVTGAHTRDIMRQVPRVLDLVGLSHKSRMYPGELSGGEQQRSAIARALVNNPKILL
                     CDEPTGNLDPTNTSEIMELLLRINLKGTTVVVATHNQAVVDRMRRRVVRLEDGRIVTD
                     EERGYYYLGLGQDQILSG"
     gene            1465..>2073
                     /locus_tag="VFU90_15685"
     CDS             1465..>2073
                     /locus_tag="VFU90_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5481287.1"
                     /translation="MNFDLTDEQKAIQSLAREFAQGEVKPRAEQMDRDEAFPYELVAK
                     MGELGFMGLPFPEEYGGAGGDTVSYALAVMEIARADASTAITMAAHVSLGATPFFLFG
                     TEEQKQRYLVPLAQGKRLWGFGLTEPNAGSDAGNVSTKAVLRDGSWFINGTKAFITNS
                     GTDISGGTTITAVTGTRPDGRPEISNLIVPQETPGFTRSKKYK"
BASE COUNT          390 a          655 c          683 g          345 t
ORIGIN      
        1 ccggaggtga cggccgccat cgccgccccg aaggtgacga gagccccgac cgcgccggcc
       61 accgcgcccg tgatcgcgcc ctcgaccacg aatgggcccc gcaccatcca gcgcgatgcg
      121 ccgaccagct gcatgatcga gatctcgcgc cgccgggcga acaccgtcag ccggatcgtg
      181 ttggagatga tgatccccgc caccgccagg aagacggcga tgacgccgat cccgacgcgc
      241 cgcaagacga ccccgagctg gagcaggcgc tgcaccacgg tttgaccgta aacgacgttt
      301 tcgatcccgt tgagccggcg aacgccggcc gccaccgccg gcacctgctc gggaacgcgt
      361 actttgatgc gaaacttgtc gggcagcggg ttttcggtga gcagcgaggt gtcgatcacg
      421 cctttcgtgc ggtcgcgcag ttcggccagc ccctgcttct tcgagacgaa ttcgatcgaa
      481 gcgattcgcg gatccgccgc cagcaggtgc cggatcgacg tcacttgcga aggcgtcgcg
      541 ccgggctgga gatacgccga aatctcgatt tgatcgagca agtgcgtccc gatctcggac
      601 agcgttccgc gcacgaagag aaagaggccg agcagaacga tcgtgatggc gaccgtgccg
      661 atggcggttg cctgcatgcc ggcgttgcgc gtgaagttgc gcagcacctc acccagaaag
      721 aatctgatcc tgccccagtc caaggtagta gtagccccgt tcttcgtcgg tgacgatgcg
      781 gccgtcttcc aaacgcacca cgcggcggcg catccggtcg acgacggctt gattgtgagt
      841 cgcgacgacg accgtcgtcc ctttgagatt gatgcggagc agcagctcca tgatttcgga
      901 ggtgttcgtc gggtcgaggt tgccggtcgg ctcgtcgcac aggaggatct tcggattatt
      961 caccagcgcg cgcgcgatcg cgctgcgttg ctgctcgccg ccggagagct cgcccggata
     1021 catgcggctc ttgtgcgaga gcccgacgag atcgagcacg cgcggtactt ggcgcatgat
     1081 gtcgcgcgta tgcgcgccgg tgacctgcat cgcgaaggcg acgttttccc agacggtctt
     1141 gtcgacgagg agtttgaaat cctgaaacac gacgccgaga tggcggcgga gggccggaac
     1201 gcgggagcgt cgcaggcggt cgacgcgaat gccgtcgacg gtgatctcgc cggtcgaggg
     1261 ccgcgcttcg cggtagagca accgcagaag gctcgacttt ccggtacccg agtgcccgac
     1321 gaggaaaacg aattcgcctt ttgcgatttc gagggttacc ttatcgatgg cacgtacgcc
     1381 gttcgggtat accagcgaga cgttgcgaag ggatatcatc gctgcgagag gagttttcct
     1441 caaggtgcga cctatacctt cgccatgaac ttcgacctga ccgacgagca gaaggcgatc
     1501 caaagcctcg cgcgcgagtt cgcgcagggc gaggtgaagc cgcgcgccga gcagatggac
     1561 cgcgacgagg ccttcccgta cgagctggtc gcaaagatgg gcgagctggg tttcatgggg
     1621 ctgccgtttc ccgaggagta cggcggcgcc ggcggcgaca cggtgagcta cgcgctcgcc
     1681 gtcatggaga tcgcgcgcgc cgacgcctcg accgcgatta cgatggccgc ccacgtctcg
     1741 ctcggggcga cgccgttttt tctattcgga acggaggagc agaagcagcg ttatctcgtc
     1801 ccgctggcgc aaggcaagcg gctctgggga tttggattga ccgagcccaa cgcgggcagc
     1861 gacgccggaa acgtctcgac gaaagccgtc ctccgcgacg gcagctggtt tattaacgga
     1921 accaaggcct tcatcaccaa ctccggcacc gacatcagcg gcggaacgac gatcaccgcg
     1981 gtgacgggga cgcgccccga cggccgcccg gagatctcga acctcatcgt ccctcaagaa
     2041 acgcccggct tcacgcgcag taagaagtat aag
//
LOCUS       DASUCJ010001347         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1481526, whole genome shotgun sequence.
ACCESSION   DASUCJ010001347 DASUCJ010000000
VERSION     DASUCJ010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481526"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFU90_15690"
     CDS             complement(<1..708)
                     /locus_tag="VFU90_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481288.1"
                     /translation="MLPRRSQPATPDGPVPRSNWQLFRERFFRHKVAWISAIVLVVLF
                     VVCFGASWVAPYPKNHIDPLLIADAHGPSAKHWFGTTDAGEDVLTQVLYGGQVSLKIG
                     LTVALLSTMVGVLVGAVAGFFGRWIDQGLSRVTDLFLIVPEIALLALALKKLGHTPSW
                     IIVVLAALGWMYIARVVRGQVMSLKEKEFVEAARATGASNRRIVLRHILPNCVGSITV
                     NMTLAVATAIVAESSLSF"
     gene            complement(762..>1115)
                     /locus_tag="VFU90_15695"
     CDS             complement(762..>1115)
                     /locus_tag="VFU90_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481289.1"
                     /translation="RFQRASMLDVMHADYVRTARAKGVPRRKVILKHAFRNACIPLVT
                     VVALDTAFLIGGLIITEQIFSIPGMGRMFFDALQSGDAPVLLGWFVVCAIAVVVMNLV
                     ADILYGVLDPRIRAA"
BASE COUNT          229 a          370 c          357 g          159 t
ORIGIN      
        1 gaacgacaac gacgactctg cgacgatcgc ggtcgcgacg gcgagggtca tgttcacggt
       61 gatcgaaccg acacagttcg gaaggatgtg ccgcagcacg atccgccggt tgctcgcgcc
      121 ggtcgcgcgc gcggcttcga cgaactcctt ttccttcagt gacatcacct gtccgcgcac
      181 gacgcgcgcg atgtacatcc atccgagggc cgcgagcacg acgatgatcc acgacggtgt
      241 gtgcccgagc ttcttcaacg cgagcgcgag caacgcgatc tccggaacga tcaagaacag
      301 gtcggtgacg cggctcaatc cttggtcgat ccaccggccg aagaagcccg cgacggcacc
      361 gacgagcaca cccaccatcg tcgacaacaa cgcgaccgtg agaccgatct tcagcgacac
      421 ctggccgccg tagagcactt gcgtcaacac gtcctcgccg gcgtcggtcg tgccgaacca
      481 gtgtttcgcg gacggaccgt gcgcgtcggc gatcaacaac ggatcaatgt ggttcttcgg
      541 gtacggcgcg acccacgacg caccgaagca cacgacgaac aacacgacga gcacgatcgc
      601 cgagatccac gcgaccttgt gccgaaagaa ccgctcccgg aacagctgcc agttcgaccg
      661 cggaacgggg ccgtcgggcg tcgccggttg cgaacgccgc ggaagcacgc gccgtcgcgg
      721 ctcaatgcgc ggcgcgtcgg tacgcgactc agtgcgcgtg ttcatgcggc acgaatccga
      781 gggtcgagca cgccgtacaa gatgtcggca acgaggttca tcacgacgac cgcgatcgcg
      841 cacacgacga accagcccag cagcaccggc gcgtcgccgc tctggagtgc gtcgaagaac
      901 atccggccca tgccgggtat cgagaagatt tgctcggtga tgatgaggcc gccgatcagg
      961 aaagcggtgt cgagcgcaac gacggtgacg agcgggatac acgcgttccg gaacgcgtgc
     1021 ttcaggatca ccttgcggcg cggcacaccc ttcgcgcgag ccgtgcgcac gtagtcggcg
     1081 tgcatgacgt cgagcatcga cgcgcgctgg aagcg
//
LOCUS       DASUCJ010001348         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_518807, whole genome shotgun sequence.
ACCESSION   DASUCJ010001348 DASUCJ010000000
VERSION     DASUCJ010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518807"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFU90_15700"
     CDS             complement(<1..491)
                     /locus_tag="VFU90_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481290.1"
                     /translation="MIVRMIVALGVAVIGTAFGDAGFGGITSAVADTLEHGTTEIALT
                     NRGDSWWVSATAIERRSPTGEPLASIRSDVSGLGTIERFAVSPHDASLWAVTDSHRLV
                     HVESKGTGVSASPLPPRVASLAVAQNGDVWVALHDAILRFAADGRPLASQPLTSHPLG
                     VGDP"
     gene            complement(518..>1753)
                     /locus_tag="VFU90_15705"
     CDS             complement(518..>1753)
                     /locus_tag="VFU90_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481291.1"
                     /translation="ATPGVFQPTWPSGGDESSFALKLNPAGSAASYATFLGGVNATSA
                     NAIAVDGQGRAHIGGWAAGDIPLVLPVQATAGGAKDGFVSVLDASGSRLVSSTWFGGF
                     NDDSVNALAIGADGAVYIAGETYSPNFPSVNGFQPVKSGYRLVNATLGNAFVAKLDLE
                     ARRIVWSSFLGGEICTGYCQALGGGIQFPGDAAHGIAVDTQGHAYVTGLARTWTFPLV
                     DSTSPRKQQDNEDSGFVAKVSVAGSTLLFSTFVRTGYSGTAVTAGFPDGSVSAVAIDR
                     AGAAYVAGDSTFSTQVTTSAGAFQSVNPGGATAIIVKFPSRSRHLTIDVANRDVDASS
                     LVTMTARVDGPETSGYVIFKSGANKLGAAPLTANLATYITPLSPGIHTLNAELSMPDG
                     NVDAPPMILVVDQPLVCSK"
BASE COUNT          305 a          569 c          593 g          286 t
ORIGIN      
        1 gggtcgccaa cgccgagcgg atgcgacgtg agcggctgtg acgcgagcgg acggccgtcg
       61 gcggcgaagc gcaggatcgc gtcgtgcaga gcgacccaga cgtcgccgtt ctgggcgacc
      121 gcgagcgacg cgacccgcgg cggaagcgga cttgcgctga cgccggtgcc tttgctctcg
      181 acgtggacga ggcgatgcga atcggttacc gcccacaggc tcgcatcgtg cggcgacacg
      241 gcgaagcgct cgatcgtgcc gaggccgctc acatcgctgc gaatgctcgc gagcggctct
      301 cccgtgggtg agcgccgctc gatggccgtc gccgataccc accatgagtc cccgcggttc
      361 gtcagtgcga tctcggtagt tccatgctcg agcgtatcgg ccactgcgct cgtgatgccg
      421 ccaaagccgg cgtcaccgaa cgcggtgccg atgaccgcga cgccgagcgc gacaatcatt
      481 cgaacgatca tggtcattgc ccgctgtcgg ttcgcgttca tttcgagcag acgaggggct
      541 gatcgacgac cagaatcatc ggcggcgcgt cgacgttgcc gtcgggcatc gagagttccg
      601 cgttcagggt atggatgccg ggagacaggg gcgtgatgta cgtcgcaagg ttcgcggtga
      661 gcggcgccgc tccaagcttg ttcgcccccg acttgaagat cacgtagccg gaggtctccg
      721 ggccgtccac gcgtgccgtc atcgtcacca gggaggaggc atccacgtcg cgattcgcga
      781 cgtcgatcgt gaggtgccgc gaccgtgacg ggaacttgac gatgatcgcg gtcgcgcccc
      841 cgggattgac ggattgaaac gcaccggccg aggtcgtcac ctgcgtactg aaggtggagt
      901 caccggcaac atacgcggcg ccggcacggt cgatcgctac cgcgctcacg ctgccgtcgg
      961 ggaagccggc tgtcacggca gtacccgagt agccggtgcg cacgaatgtc gagaagagga
     1021 gggtggatcc cgcaaccgat actttcgcca cgaacccgga atcctcgttg tcctgctgct
     1081 tgcgcggaga cgtggaatcg acgaggggaa acgtccacgt acgtgccagc ccggtcacgt
     1141 aggcgtgtcc ctgcgtgtcc acggcgatgc cgtgcgcggc gtcgcccgga aactggatgc
     1201 cgcccccgag cgcctggcaa tagccggtgc agatctctcc gccgaggaac gacgaccaga
     1261 cgatccgccg cgcctcgaga tcgagcttcg cgacgaaggc gttgccgagc gtcgcattga
     1321 cgagccggta acccgacttc actggctgga agccgttgac gctcggaaag ttcggcgaat
     1381 aggtctcgcc ggcgatgtac acagctccgt ccgcgccgat cgccagcgca ttcaccgagt
     1441 cgtcgttgaa accgccgaac catgttgacg agacgaggcg gctccccgac gcgtcgagta
     1501 cgctgacgaa gccatcttta gcgcccccgg cagttgcttg caccggcaac accagtggaa
     1561 tgtcgcccgc tgcccagccg ccgatatgtg cgcggccttg cccatcgacc gcgatcgcgt
     1621 tcgcggacgt tgcgtttacg ccaccgagaa acgtcgcgta ggaggctgcg gagcccgcgg
     1681 gattcagctt cagcgcgaaa ctggattcat ctccgccgga tggccaggtt ggctggaata
     1741 ccccgggagt cgc
//
LOCUS       DASUCJ010001349         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1328811, whole genome shotgun sequence.
ACCESSION   DASUCJ010001349 DASUCJ010000000
VERSION     DASUCJ010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328811"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFU90_15710"
     CDS             complement(<1..297)
                     /locus_tag="VFU90_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5481292.1"
                     /translation="MASTGELVPGTLEMLILKTLSLGSLHGYGIAQHIHRLSDEALTV
                     EEGSLYPALQRMLAKGWVAAEWKQTPTKRRARYYRLTAAGRKQLGVESSAFERAM"
     gene            complement(386..811)
                     /locus_tag="VFU90_15715"
     CDS             complement(386..811)
                     /locus_tag="VFU90_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5481293.1"
                     /translation="MLGTRAFGTSGLTVSAMGLGLMGMSYAYGTTADRDERESIATIH
                     RTIELGVTLLDTAEAYGPYENEKLLARALRSLGGDARDRVVIATKFGFTFADGAINGV
                     DSRPAHLRQVVDASLERALTPGQVAGPRYGVAQMAQIDR"
BASE COUNT          166 a          335 c          359 g          178 t
ORIGIN      
        1 catcgcccgc tcgaatgccg acgactcgac gccgagctgt ttgcggccgg ccgccgtcag
       61 gcgatagtac cgtgcgcgac gcttggtggg cgtctgcttc cactctgcgg ccacccaacc
      121 cttggcgagc atgcgttgca gcgcgggata gagcgagcct tcctcgacgg tgagcgcctc
      181 atccgacagt cgatggatgt gctgcgcgat gccgtagccg tgcagcgacc ccaggctcag
      241 cgtcttgagg atgagcatct cgagtgttcc gggaaccagc tcgccggtgg aggccatata
      301 tagatgctct atgggttgac cacctgatga tacggtcggg acgatcgatc gtcaagtcgt
      361 ccatgcgccc gagcggacgg agcgcttacc gatcgatctg cgccatctgc gcgacgccgt
      421 agcgcgggcc ggcgacctga cccggcgtga gcgcgcgctc gagcgacgcg tcgacgacct
      481 gccggaggtg cgcgggtcga ctatccacgc cgttgatcgc gccgtcggcg aacgtgaagc
      541 cgaacttggt cgcgatcacc acgcggtctc gtgcgtcgcc gcctaacgac ctgagcgcgc
      601 gcgccagcag cttctcgttc tcgtatggcc cgtaggcttc cgccgtgtcg agcagcgtga
      661 cgccgagctc gatcgtgcga tggatggtcg cgatggattc gcgttcgtcg cgatcggcgg
      721 tggtgccata ggcgtagctc atgcccatga ggccgagccc catggcggag acggtgaggc
      781 cgcttgtgcc gaacgcgcgg gtgccgagca ttggtcgata ccggtggcgg gtggttgggt
      841 gcgccgccga tcggacggac ggacggcacg ggcgcaccgg gcgccgcacc gaccattcgg
      901 ccgccggaac gtgcgtgcct cggcgaaaaa cgcgcgcgac ggtcgacggc gcgccccgat
      961 gcgggctcat caacgagttt ttacctacaa agtaggcaaa aactctctga tgccacgtga
     1021 agcgccgtcg gctccgat
//
LOCUS       DASUCJ010001350         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_931420, whole genome shotgun sequence.
ACCESSION   DASUCJ010001350 DASUCJ010000000
VERSION     DASUCJ010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931420"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..693
                     /locus_tag="VFU90_15720"
     CDS             91..693
                     /locus_tag="VFU90_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5481294.1"
                     /translation="MLAQVLTITALGLCVTALAAWLAQGVPAGLGLGAMIVGFILLLA
                     INWTRANEPLSLVLFYAFAFCEGVGIAPVIGQYLRTVGSSVVVNAALTTGLGMLVLAA
                     VVYATGLDLRRFQGIFMIALLGLIVLGIVSIFVRFLHPETYAWLTLVIFAGLVLIDFA
                     RIRAGGDGLTPVQMAVSIYLDAINIFLALLQIFGGRRSQD"
     gene            762..>2031
                     /gene="purF"
                     /locus_tag="VFU90_15725"
     CDS             762..>2031
                     /gene="purF"
                     /locus_tag="VFU90_15725"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5481295.1"
                     /translation="MCGITGVFAPGRDAARLAFFALYALQHRGQESAGIAAADGGTIR
                     SHKEMGLLSAIFDEDILSDLSGYIAIGHTRYSTTGSSIVVNAQPLLERTELGDFAFAH
                     NGNVTNTDELRARLSPTTVLQASSDSEVLAKLIVESKGTMVDRIKALLSAARGAYSIV
                     LCTQDELYAFRDPWGVRPLCLGTLPDGGYVVASESCALGTIGAQYLREVGRGEIVRLS
                     TRGLESYETDVERATPALCMFEYIYFARPDSKLNDRSIYMARYAMGRELAKEHPVDAD
                     AVMAVPDSAVPGGIGYAAESGLPYVEGLIKNRYIGRTFISPDQAMRSRGVHLKFNPVV
                     ENLTGQRVIVVDDSIVRGTTTPRIVALLREAGASEVHLRITSPPIKHPCYLGVDMATY
                     DELIAANYTVEEIRRRTGADSLGYLSLKGLI"
BASE COUNT          341 a          639 c          659 g          392 t
ORIGIN      
        1 gttattagct agcgtatggg tcttcaattc caacggcgcc cgggcagtcc gtacggtgcc
       61 gccatcgctc cggcaatacc gacgcaatcg ttgctggcgc aggtcttgac catcaccgcg
      121 cttgggttgt gcgtcaccgc gctggcggcg tggctcgcgc agggcgtacc ggcggggctt
      181 ggtttgggag cgatgatcgt cggcttcatt ttgctgctcg cgatcaactg gacgcgcgcc
      241 aacgagccgc tgtcgctggt gctgttctac gcatttgcgt tctgcgaagg cgtgggaatc
      301 gcgcccgtca tcgggcagta tttgcggacc gtcggctcga gcgtcgttgt caacgccgcg
      361 ctgacgacgg ggctgggcat gctcgtgctg gcggcggtgg tctacgccac cggactcgac
      421 ctgcgccgtt tccaggggat cttcatgatc gccctactcg ggctgatcgt cctcggcatc
      481 gtctcgatct tcgtccggtt cctgcacccc gagacgtatg cgtggctgac gctggtcatc
      541 ttcgcgggtt tggtgctgat cgatttcgcc cgcattcggg ccggcggaga cggcctgacg
      601 ccggtacaaa tggccgtgag catctacctc gacgccatca atatcttcct tgccttgctg
      661 cagattttcg gaggccggcg ttcgcaagac taagcggcgc ccggtgcact cctcgaaagt
      721 cgggggcgcg accgccctag gcctggtata ataaaaacac gatgtgcggg ataacggggg
      781 tttttgcgcc gggacgcgac gcagcccgtc tcgcgttttt cgcgctttac gcgctgcagc
      841 atcgcggcca agagtcggcg ggcatcgccg ccgccgacgg cggcacgatc cgttcgcaca
      901 aagagatggg gctgttgagc gccatcttcg acgaagacat cctcagcgac ttgagcggct
      961 acatcgcgat cggacatacg cgttactcga cgaccggctc gtcgatcgtc gtcaacgcgc
     1021 agccgctgct cgagcgaacc gaactcggcg acttcgcatt tgcccataac ggcaacgtca
     1081 ccaacaccga cgaattacgc gcgaggctgt cgccgacgac ggtcctgcag gccagctcgg
     1141 actccgaggt gctggcgaag ctcatcgtcg agtcgaaggg cacgatggtc gatcgcatca
     1201 aagcgctgct gtcggcggcc cgcggtgcgt attcgatcgt gctgtgtacg caggacgagc
     1261 tgtacgcgtt tcgcgacccg tggggcgtgc gtccgctctg cttggggacg cttccggacg
     1321 gcggctacgt agtcgcttcc gaatcgtgcg cgctcggaac gatcggcgcg cagtatctgc
     1381 gcgaggtcgg acgcggcgaa atcgtacggc tctcaacgcg cgggctcgaa tcgtacgaga
     1441 ccgacgtcga gcgggcgacg ccggcactgt gcatgttcga atacatctac ttcgcgcgtc
     1501 ccgattcgaa actcaacgac cgttcgatct acatggcgcg ctacgcgatg gggcgcgaac
     1561 tggccaaaga gcatccggtc gacgccgacg ccgtaatggc ggtgccggac tccgcggtgc
     1621 cgggcggcat cgggtacgcg gccgaaagcg gcttgccgta cgtcgaagga ctgatcaaga
     1681 accgctacat cgggcgcacc ttcattagcc ccgatcaagc gatgcgctcg cgcggcgtcc
     1741 atctgaagtt caatccggtg gtggaaaatc tcaccggaca gcgggtgatc gtcgtcgacg
     1801 attcgatcgt gcgtggaacg acgacgccgc gaatcgtcgc tctgctgcgc gaggccggcg
     1861 cgtccgaagt gcacctgcgg atcacgtcgc cgccgatcaa acacccgtgc tatttgggcg
     1921 tcgacatggc gacctacgac gaactcatcg cggccaacta tacggtcgag gagatccgcc
     1981 gccgcaccgg cgccgattcg ctcggctatc tgagcctgaa ggggctcatc g
//
LOCUS       DASUCJ010001351         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1405157, whole genome shotgun sequence.
ACCESSION   DASUCJ010001351 DASUCJ010000000
VERSION     DASUCJ010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405157"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1681)
                     /locus_tag="VFU90_15730"
     CDS             complement(<1..>1681)
                     /locus_tag="VFU90_15730"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEU5481296.1"
                     /translation="AETGPLAGSWHWHRFMNPARDGVVLPILHLNGYKLSGPSLLARF
                     SVNALAEYFAGMGYEAREVAGDERALVHRAMWDALDWAYDRLGEHRRAARNGGASDQP
                     WPLIVLRTPKGWTGPKSLDGQAIENTFHAHQIPIEDPAANPDHLRALERWLRSYHPEE
                     LFDADGRPAADVTAMYPVGDMRLGACPAANGGALLVPLVCPDPTPVSVDAAAPGETII
                     ENTKVLGGYLREVFNANEDAANFRLFCPDETSSNRLQNVFESTLRAWMLPLVPTDEYL
                     SMTGRVLEILSEHCCEGWLEGYVLSGRHGIFACYEAFLTIVDSMVQQFAKWQKMAAEV
                     TWRVPTASLNLLVTSHVWEQDHNGYSHQGPSLMNLLVTKKAATTRVFLPPDANSLLAV
                     TRRCLESRGDINLIVASKKPMPQWLDVDEADAQCSAGASHWAWAGTDPSHADVVLAGA
                     GDVPARESVAAAWWLKRVAPDLRVRVVNIIDLLALESPDDNPHGIDAQRFDALFGATV
                     PVVMAFHGYPSAVHEIIHHRPHPQRFHVRGYVEEGTTTTPFNMLVLNGMSRF"
BASE COUNT          269 a          569 c          566 g          277 t
ORIGIN      
        1 aaagcggctc atgccgttga ggacgagcat gttgaacggc gtggtcgtcg tcccttcctc
       61 gacgtacccg cgcacgtgga agcgctgcgg atgcggccgg tggtggatga tctcgtgcac
      121 ggcgctcgga tacccgtgaa acgccatcac gacgggcacg gtcgcgccga acaacgcatc
      181 gaatcgttgg gcatcgatgc cgtgcgggtt gtcgtccggc gactcgagcg ccaacaagtc
      241 gatgatgttg acgacgcgca cgcgcagatc gggcgcgacg cgcttcagcc accaggcggc
      301 agccacggat tcccgcgccg gcacgtcgcc ggcgcccgcg agcacgacgt ccgcatgcga
      361 tgggtcggtg cccgcccacg cccaatgcga tgcgccggcc gagcactgcg catccgcttc
      421 gtccacgtcg agccattgcg gcatcggttt cttcgacgcg acgatgaggt tgatgtctcc
      481 ccggctctcg agacaccggc gagtaacggc gagcagcgag ttcgcatcgg gcggcaagaa
      541 cacgcgcgtc gtcgccgcct tcttggtgac caggaggttc atcaacgacg ggccttgatg
      601 gctgtagccg ttgtgatcct gctcccacac gtggctggtc accagcaggt tcagcgacgc
      661 ggtcggcacg cgccacgtca cctcggctgc catcttctgc catttggcaa actgctggac
      721 catcgagtcg acgatggtga ggaacgcctc gtagcacgcg aagattccgt ggcggcccga
      781 cagcacgtat ccctcgagcc agccctcgca gcagtgctcg ctcaggattt cgagcacccg
      841 gccggtcatc gagaggtatt catcggtcgg cacgagcggc agcatccagg cgcggagcgt
      901 cgactcgaac acgttctgca gccggttgga cgacgtctcg tcggggcaga agagccggaa
      961 gttggccgcg tcctcgttgg cgttgaacac ttcgcgcagg tagccgccta acaccttggt
     1021 gttctcgatg atcgtctcgc cgggggcggc ggcgtccacc gacaccgggg tggggtccgg
     1081 gcacacgagc ggcacgagca gcgcgccgcc gttcgccgcc ggacaggcgc ctaaccgcat
     1141 gtcgcccacc ggatacatcg cggtcacgtc cgccgcgggg cggccgtcgg cgtcgaacag
     1201 ctcctccggg tgatagctgc gcagccagcg ctcgagcgcg cgcagatggt ccgggttggc
     1261 ggccgggtct tcgatcggaa tctggtgcgc gtggaacgtg ttctcgatgg cctggccgtc
     1321 gaggctcttg ggcccggtcc aacccttcgg cgtgcgcagc acgatgagcg gccacggctg
     1381 gtcgctcgcg ccgccgttgc gcgccgcccg gcggtgttcg cctaaccgat cgtacgccca
     1441 gtcgagcgcg tcccacatcg cgcggtgcac gagcgcgcgc tcgtcgcccg cgacttcccg
     1501 cgcctcgtat cccattcccg cgaaatattc ggcaagtgca ttgacgctga accgcgccag
     1561 cagcgacggt ccggagagct tgtagccatt gaggtgcagg atcggcaaca cgacgccgtc
     1621 gcgggcggga ttcatgaacc gatgccagtg ccacgacccg gcaagcggac cggtctcggc
     1681 c
//
LOCUS       DASUCJ010001352         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1084283, whole genome shotgun sequence.
ACCESSION   DASUCJ010001352 DASUCJ010000000
VERSION     DASUCJ010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084283"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFU90_15735"
     CDS             complement(<1..736)
                     /locus_tag="VFU90_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481297.1"
                     /translation="MSSSLVITAADKSGRWYVLPGTSNSYDLTVANQSDHAVLCKLTL
                     DEPGDAGAVSPSSLTLKGGESRNVSVAFKPEWLTLRDRKAVVTARDAAGSVLATFVGD
                     LVAATATDCSVSLAWKDEIGGDGSLRGFILGCTVRSISSMPGVFEPEFTPHPSLRFPE
                     TQRITLGPGESSTFDVPIVWNRSSRDNEGWNHPRSIEVVVPVTHGRRSATAPWDLVQH
                     HIEPHLTDADKSPVIVRRPPPPQFVQP"
BASE COUNT          176 a          331 c          337 g          175 t
ORIGIN      
        1 cgggctgcac gaactgcggc ggcggcggcc ggcgcacgat gaccggcgac ttgtcggcgt
       61 cggtgagatg cggctcgatg tgatgctgga cgagatccca cggtgccgtc gccgagcggc
      121 gcccgtgcgt gacgggcacg acgacttcga tcgatcgcgg atggttccaa ccctcgttgt
      181 cgcgcgacga acgattccag acgatcggca cgtcgaatgt cgacgactcg cccgggccga
      241 gcgtgatgcg ctgcgtttcg gggaagcgga gcgacggatg cggcgtgaac tccggttcga
      301 agacgccggg catcgagctg atgcttcgca ccgtgcaccc gaggataaat ccgcgcagcg
      361 agccgtcgcc gccgatctcg tccttccacg cgagcgatac gctgcagtcg gtggcggtcg
      421 cggcgacgag atcgccgacg aaggtcgcga gcacgctgcc tgccgcgtcg cgtgcggtga
      481 cgacggcctt gcggtcgcgc agcgtcagcc attcgggctt gaacgcgacc gacacgttgc
      541 gagactcgcc gcccttgagc gtgagcgatg acggagagac cgctccggcg tcgcccggct
      601 cgtcgagcgt cagcttgcac agcacggcgt ggtcggactg gttcgcgacg gtcaagtcgt
      661 acgaattgga cgtccccggc aagacatacc acctgccgga cttgtccgcc gctgtgatga
      721 cgaggctcga actcatgctc ctctactata tatcgacccg gtcagggcgc accgccatcg
      781 taccgccgga aacgttggga aatgattaag aaaacctcgc ctccaaggag tggcgcccgt
      841 cacgcgccca agaaatcggg tcccgtgaac aattttttga atcgcgagct gtcgtggttg
      901 gacttcaaca gccgtgtcct cgcgctcgcg gaagacccgg ccgtgccgct gctcgagcgc
      961 gtcaacttcc tcgcgatctt cagccggaac ctcgacgagt tcttccagat ccgcgtcgc
//
LOCUS       DASUCJ010001353         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1190896, whole genome shotgun sequence.
ACCESSION   DASUCJ010001353 DASUCJ010000000
VERSION     DASUCJ010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190896"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFU90_15740"
     CDS             complement(<1..292)
                     /locus_tag="VFU90_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481298.1"
                     /translation="MGGGVEQWALSFASTMHWFGEQLDVSHTWHDAAVASAQSTSTTP
                     QPQLHVPSPYVEAMHVQSAGMAHFALSFDAIEEGHGPFAHDPAAMHCWQFATP"
     gene            717..1058
                     /locus_tag="VFU90_15745"
     CDS             717..1058
                     /locus_tag="VFU90_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481299.1"
                     /translation="MCNCGCAVPNQPCQGITLEYVCEGYPADGCTAGIPSHLNPMNNG
                     VCTVNVPSTWTCSPSYYDDGLCDCGCGAVDLDCPTNDVTHCKTCNDPGSCSASGCPGS
                     IVADDTAHCSN"
     gene            complement(1068..>1394)
                     /locus_tag="VFU90_15750"
     CDS             complement(1068..>1394)
                     /locus_tag="VFU90_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481300.1"
                     /translation="TADGRPFLGNYMATTAPSSVLPGIVNQPSYEDPKLDIVKAGDAT
                     NSWLVIKLLGTQNDHLTDCMSGKNAYGTGGPCGSQMPGAGMAAALDTATITLIENWIN
                     QGAQNN"
BASE COUNT          213 a          469 c          465 g          247 t
ORIGIN      
        1 caggcgttgc gaactgccag cagtgcatgg cggccgggtc gtgtgcgaat gggccgtgtc
       61 cctcttcgat tgcgtcgaac gacaacgcga agtgcgccat ccccgccgac tggacgtgca
      121 tcgcttcgac gtacggcgac ggcacgtgca actgcggctg cggcgtcgtc gacgtggact
      181 gcgccgacgc cacggcggcg tcgtgccagg tgtgcgatac gtcgagttgc tcgccgaacc
      241 aatgcatggt ggaggcgaac gacaacgccc attgctccac gcctccgccc atgtggcgat
      301 gccccgcgcg gctctatcac gacgggctgc agtgcgactg cgggtgcggc gcggccgatc
      361 ccgattgcgc ttcgatcagc gagacggttt gcgaccgctg cgacgatccc ggctcgtgtt
      421 ccgcgcaggc ctgccctggc ctggtcgatc ctcggcaaaa ctaccgctgc gatcagccga
      481 cgcctcccgc gggatggact tgcccagcga acgcttatgg cgacgggatc gaatgcgatt
      541 gtggatgcgg cgcgcccgac cccgactgcg catcgagcgc cgtcagcgat tgcgcgcgat
      601 gcctcgtctg cggcggccac ggcgattgcg tcggcaccgt cgacaccggg gacaccacgc
      661 aatgcgcgcc gccccccagc ggctggacgt gctcggcggc cgagtggcgc gcggcggtgt
      721 gcaactgcgg ttgcgccgtc cccaaccagc cttgccaagg catcacgctc gagtacgtct
      781 gcgagggcta ccccgcggac ggctgcaccg cgggcattcc ttctcatctc aatccgatga
      841 acaacggggt gtgcacggtc aacgtgccga gcacctggac gtgcagcccc agttattacg
      901 acgacggcct ttgtgactgc ggctgcggcg ccgtcgacct cgattgcccg acgaacgacg
      961 tcacccactg caagacttgc aacgaccccg gaagctgctc cgcgagcggg tgtccggggt
     1021 cgatcgtcgc ggatgacacc gcgcactgtt cgaactgagc ctgcgcgtca gttgttctgc
     1081 gcgccctggt tgatccagtt ttcgatgagc gtgatcgtcg ccgtgtcgag cgccgcggcc
     1141 atgcctgcgc ccggcatctg cgagccgcag gggccccccg ttccgtaggc gttcttgccg
     1201 ctcatgcaat cggtcaaatg atcgttttgc gtgccgagga gcttgatgac aagccagctg
     1261 ttcgtcgcat cacccgcctt cacgatgtcg agcttcggat cttcgtagga cggctggttc
     1321 acgatgccgg gcaataccga ggacggcgcc gtggtcgcca tgtagttgcc gaggaagggg
     1381 cggccgtcgg cggt
//
LOCUS       DASUCJ010001354         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_901082, whole genome shotgun sequence.
ACCESSION   DASUCJ010001354 DASUCJ010000000
VERSION     DASUCJ010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901082"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFU90_15755"
     CDS             complement(<1..519)
                     /locus_tag="VFU90_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5481301.1"
                     /translation="MLLRHFGYGRYGPARRGKRLRPHMVLQVATAEGAPVEHALDAAV
                     AVEIFHNYSLVHDDIEDRDELRHGRKTLWSAYGVPQAINAGDAMCALSFLSLEHAADF
                     VAAGRVLQMIALLHEAHAVMCEGQSLDLRFETETCVDLDGYHRMIECKTAQLFDASCR
                     LGALCAGGDAATV"
     gene            complement(584..1426)
                     /gene="ispH"
                     /locus_tag="VFU90_15760"
     CDS             complement(584..1426)
                     /gene="ispH"
                     /locus_tag="VFU90_15760"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5481302.1"
                     /translation="MEIRKASVQGFCFGVAITVKKAEEAIASRGEVTTLGHVVHNPQM
                     VESLSARGLRNAQSVDDVDSGSLFVRAHGLPVDVFEKAKDKGLEIFDASCPMVTKIHV
                     QAEKLKSDGYKIVVIGDPAHPEVKGTLSHVPGAWCIQKVDDVARLPRSSKVGVVVQST
                     WSGQGFTEIVQALAAKYYEVRAVNTICTDTHNRQDEALRLARDVEVMVVVGGKTSANT
                     KHLAELSESHGAHAYHIEGAAELRPEWFEGVKGAGLMSGASTPGWLVDEVEARMEELS
                     RQPA"
     gene            complement(1413..2012)
                     /locus_tag="VFU90_15765"
     CDS             complement(1413..2012)
                     /locus_tag="VFU90_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5481303.1"
                     /translation="MKPPLYDFAAFTLRVMFRTLFRARAAGVENVPRDGPLIVAINHV
                     SYLDPPALCFCPRRVSFMAKKELFDIPVLGPLIRGVGAYPVDRRGSAKTAIKRSLAVL
                     EAGGAVGIFPEGTRNLTGEAQPQTGVALLAALSGAPVLPTAVIGSDRAARLGKIKVVF
                     GRPLALAREGKATREDLAKFTDQVMNAIRALAGSAGGNT"
BASE COUNT          379 a          759 c          666 g          391 t
ORIGIN      
        1 gacggtggcg gcgtcgccgc cggcgcagag cgctcccagc cggcaggagg cgtcgaacag
       61 ctgcgccgtc ttgcattcga tcatccggtg atagccgtcg agatcgacgc acgtctccgt
      121 ttcgaatcgc agatccagcg attgcccctc gcacatcacc gcgtgcgctt cgtgcagcag
      181 cgcgatcatc tgcagcacgc ggcccgcggc gacgaaatcg gcggcgtgct ccaggctgag
      241 aaagctgagc gcgcacatcg cgtcgccggc gttgatcgct tgcggcacgc cgtacgcgct
      301 ccacagcgtc tttcgcccgt gccgcagctc gtcgcggtct tcgatgtcgt cgtgcaccag
      361 cgagtagttg tgaaagattt ccaccgcgac cgctgcgtcg agcgcgtgct cgaccggcgc
      421 gccttcggcg gtcgcgactt ggagcaccat gtgcgggcgc agccgtttac cgcgccgtgc
      481 cgggccgtac cggccgtaac cgaagtgacg caaaagcata tcggagatct ccctcgatcc
      541 ccgatagctg gtaatccgtt cttcgagggc gctttcgaag cgctcaagca ggttgacgac
      601 tgagctcctc catccgcgcc tcgacctcgt cgacgagcca tcccggggtc gacgcgcccg
      661 acatcaagcc ggcccctttc acgccttcga accactccgg gcgcagctcg gccgcgccct
      721 cgatatggta agcgtgcgcg ccgtgcgatt cggacagctc cgcgaggtgc ttggtgttcg
      781 ccgacgtctt gccgccgacg acgaccatta cttcgacgtc gcgtgccagc cgcagcgctt
      841 cgtcttggcg gttgtgggtg tcggtacaaa tcgtgttgac cgcgcgcact tcgtaatact
      901 tcgcggccag cgcttgaacg atctcggtaa agccttggcc cgaccaggtc gactgcacca
      961 ctacgccgac tttgctcgag cgcggcaaac gcgcgacgtc gtcgactttc tggatgcacc
     1021 acgcacccgg aacgtggctg agcgttccct tgacttccgg atgcgccgga tcgccgatca
     1081 cgacgatctt gtaaccgtcc gatttgagct tctcggcttg gacgtgaatt ttcgtcacca
     1141 tcgggcagct ggcgtcgaag atctcgaggc ctttgtcctt agccttctcg aacacgtcga
     1201 ccggcaggcc gtgcgcgcgc acgaacagcg accccgaatc gacgtcgtcg accgactgcg
     1261 cgttgcgcag cccgcgggcg ctcagcgact cgaccatctg cggattgtgc accacgtggc
     1321 cgagggtcgt cacctcgccg cgcgacgcaa tcgcttcctc ggccttcttc accgttattg
     1381 cgacgccgaa gcagaatccc tgaaccgacg ctttacgtat ttccaccggc actcccagcc
     1441 aacgcgcgta tggcgttcat cacttgatcg gtaaacttcg ccagatcctc gcgagttgct
     1501 tttccttccc gtgcgagcgc cagcgggcgc ccgaatacga ctttaatctt acccaagcga
     1561 gccgcccggt cgctgccgat gacggcggtc ggaagcaccg gcgcccccga gagcgccgcc
     1621 agcagcgcga cgcccgtttg cggctgcgcc tcgccggtga ggttgcgcgt cccctcggga
     1681 aagatgccca ccgcgccgcc cgcttcgagg acggccagcg agcgctttat cgccgttttc
     1741 gcactccctc ggcgatcgac cggataggcc cccaccccgc ggatcagcgg cccgagcacc
     1801 ggaatatcga acagctcctt tttcgccatg aagctcacgc gccgcggaca aaaacacaac
     1861 gccggcggat cgaggtacga aacgtgattg atggcgacga tcaacggtcc gtcgcgcggc
     1921 acgttttcca cgcccgcggc gcgcgcgcga aacagcgtgc gaaacatcac gcgcagcgtg
     1981 aacgccgcaa aatcgtacag cggcggcttc acgtcaccat gtcatcctga gcgtagtcga
     2041 agggctgagc ggagccaact gtgtcatcct gagcggagcg agcgcagcga gcgtagtcga
     2101 agggctgagc ggagccaact gtgtcatcct gagcggagcg agcgcagcga gcgtagtcga
     2161 aggacgaaag agcgcacctc atcgcctgcc ctccg
//
LOCUS       DASUCJ010001355         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_29625, whole genome shotgun sequence.
ACCESSION   DASUCJ010001355 DASUCJ010000000
VERSION     DASUCJ010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29625"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFU90_15770"
     CDS             <1..417
                     /locus_tag="VFU90_15770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU5481304.1"
                     /translation="DTFPARVRDAFEEWSDLGIPVRFQFVTDSLDADVHVAWIDHFDS
                     PISGKTLWARDRSWWIVNANITLALHHNAGEALDDSSVKAIALHEVGHLLGLDHTSDT
                     TDIMTARVRVRDLSNADRATMKLLYSLPPGSIRQGK"
     gene            complement(476..>1093)
                     /locus_tag="VFU90_15775"
     CDS             complement(476..>1093)
                     /locus_tag="VFU90_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5481305.1"
                     /translation="KDVTAGCEPFSTLLKPGAHVLLRVKRGAYTKQLSALVGKRPASS
                     WSQVWTVPPGSRYLIVPNAPQPQATPAPPAPPARESDYDSTQIQIAVAPFPAVSPMPE
                     ISTTVTRFGGALLVVAGAEVRPVGNLADYFGVGHGVLVLRVESGTVAARSGLEDGDVI
                     VRADGNPVDSPAGLSRAMYRASDSRLKLDIVRLKKKRTIVLKWDR"
BASE COUNT          179 a          365 c          372 g          177 t
ORIGIN      
        1 gacacgttcc cggcacgcgt gcgcgatgcg ttcgaggagt ggagcgatct cggcatcccg
       61 gttcgcttcc agttcgtgac cgattcgctc gacgcggacg tccacgtcgc ctggatcgat
      121 cacttcgact caccgatcag cggcaaaacg ctctgggcgc gcgaccgcag ctggtggatc
      181 gtcaatgcga acatcacgct cgcgttgcac cacaacgccg gcgaggcgct ggacgattcg
      241 agcgtcaagg ccatcgcgct ccacgaggtc ggccacctgc tgggcctcga ccacacgagc
      301 gacacgaccg acatcatgac ggcgcgggtc cgtgtgcgcg acctgtcgaa cgccgatcgt
      361 gcgacgatga agctgctcta ctccctgccg ccaggcagca ttcgccaggg caagtaggca
      421 gcggccagtt gcctaacgca gcgcgcgccg gcgatccacg ccggcgcgcg ctttttcatc
      481 ggtcccactt gagcacgatc gttcgtttct ttttgaggcg gacgatgtcc agcttgagtc
      541 gcgaatcgga tgcgcggtac atggcgcggc tcaacccggc cggcgagtcg actggattgc
      601 cgtcggcgcg aacgatcacg tcgccgtcct cgagtccgga acgagcggca acggtccccg
      661 actcgacgcg cagcaccagc acgccgtgtc ccacgccgaa gtagtcggcc aggttgccta
      721 ccggcctcac ctcggcgccg gcgacgacga gcagcgctcc gccgaatcgg gtgacggtcg
      781 tcgagatctc gggcatcggc gacaccgccg ggaacggggc gactgcgatc tggatctggg
      841 tcgagtcgta gtccgattcg cgcgccgggg gtgcgggcgg cgccggcgtc gcttgcggct
      901 gaggagcgtt cggaacgatg aggtatcgcg agccgggcgg gacggtccag acctggctcc
      961 acgaggacgc cggccgcttg ccgacgagcg cgctcagttg cttggtgtag gcgccgcgct
     1021 tgacgcgcaa aagcacgtgt gcgcccggct tgagcagcgt ggaaaagggt tcgcagccgg
     1081 ccgtcacatc ctt
//
LOCUS       DASUCJ010001356         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_564685, whole genome shotgun sequence.
ACCESSION   DASUCJ010001356 DASUCJ010000000
VERSION     DASUCJ010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564685"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /gene="ppk1"
                     /locus_tag="VFU90_15780"
     CDS             complement(<1..1953)
                     /gene="ppk1"
                     /locus_tag="VFU90_15780"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU5481306.1"
                     /translation="MIKPSVLPEPRLSPVPVDDPSLYISRELSWLEFNDRVLEEALDP
                     RNPLLERLKFVAIYGTNLDEFFMIRVAAIKQQIEAQVVRRSDDGRMPAEHLSAISERL
                     RTSLHTQMRLLNDDLLPALEREGIRLMRVSDLDDETQTALERTFDDTVFPVLTPLAVD
                     SGHPFPYISNLSLSLAVELEETTRDGIELHFARVKIPPTLPRLIPVETAPPGERRFVL
                     LEDLIAHHLDGLFPGMHVRDAYLFRVTRDADLDLQEDEADDLLREIESELQRRRFGEP
                     VRLELERGMPEYLRDFLCEALELTAVDSYDVDGFMALSDLWHIVNLPGYEQLRDKPFL
                     PAIPKRLIGVTDIFAAIREGDILLHHPYESFDPVVQFLQAAAEDEKVLAIKATLYRTS
                     GKKSPITRALLKAAENEKQVAVVIELKARFDEENNIEWAKRLERAGAHVVYGFANQKV
                     HAKSLLVVREDEDGLRRYMHFGTGNYNEKSARLYTDLSLFTCRPELGADITQLFNALT
                     GFSRITDYEDIWVAPVTLRRELISTIERETEHAKAGRRCGIRAKLNHISDAEVIRALY
                     RASQAGVKIDLLVRGMCVLRPGVPGLSERITVRSIVGRFLEHSRIYTFENGGDREVFI
                     ASADWMARNLDGRVEMVTPVLDPMIAE"
BASE COUNT          377 a          668 c          728 g          390 t
ORIGIN      
        1 ttcggcgatc atcgggtcaa gcaccggggt caccatttcc acccggccgt cgaggttgcg
       61 ggccatccag tcggcgctcg cgatgaagac ttcgcggtcg ccgccgtttt cgaacgtgta
      121 gatgcgcgag tgctccagga aacgcccgac gatgctgcgc acggtgatcc gctcgctcaa
      181 accgggaacg cccggacgca acacgcacat tccgcgcacc agcaagtcga tcttgacgcc
      241 cgcctgcgac gcacggtaga gggcgcgaat gacttccgcg tcgctgatgt ggttgagctt
      301 cgcgcggatg ccgcagcggc gtcccgcttt tgcgtgctcg gtttcgcgct cgatcgtcga
      361 gatgagttcg cgccgcagcg tcaccggcgc gacccaaata tcctcgtagt cggtaatccg
      421 cgagaagccg gtcagcgcgt tgaacagctg ggtgatgtcc gcgcccagct ccgggcggca
      481 ggtgaacagg ctcaggtcgg tatacaggcg ggccgacttc tcgttgtagt tgccggttcc
      541 gaaatgcatg taccggcgca gtccgtcctc gtcttcgcgc acgaccagca gggatttggc
      601 gtgaaccttc tggttggcga acccgtagac gacgtgggcg ccggcgcgct ccaagcgctt
      661 ggcccattcg atgttgtttt cttcgtcgaa ccgcgccttg agctcgatga ccacggcgac
      721 ttgcttctcg ttctcggccg ctttgagcag cgcgcgcgtg atcggcgact tcttgccgga
      781 ggttcggtac agggtcgcct tgatggcgag caccttctcg tcttcggcgg cggcctgcag
      841 aaactgcacg acggggtcga acgattcgta cggatggtgc agcaggatat cgccttcgcg
      901 aatggcggcg aagatgtcgg ttaccccgat caagcgtttg gggatcgccg gaaggaacgg
      961 tttgtcgcgc agctgctcgt agcccggcag gttgacgatg tgccacaggt cggagagcgc
     1021 catgaacccg tcgacgtcgt agctgtcgac ggcggtaagc tcgagcgctt cgcacaaaaa
     1081 gtcgcgtaag tattcgggca tcccgcgctc gagctcgaga cgcaccggtt cgccgaagcg
     1141 gcgccgctgc agctcggact cgatctcgcg cagcaaatcg tccgcttcgt cttcctggag
     1201 gtcgaggtcg gcgtcgcgcg tgacccggaa aagatacgcg tcgcggacgt gcatccccgg
     1261 aaacagcccg tcgagatggt gtgcgatcaa gtcctcgagc aagacgaaac gccgctcgcc
     1321 ggggggcgcc gtttcgaccg gaatcagtcg cgggagagtc gggggaatct tgacgcgcgc
     1381 gaagtgcagc tcgatgccgt cgcgcgtcgt ttcttcgagc tcgacggcca gcgagagcga
     1441 caagttcgag atgtacggga acggatgtcc gctgtcgacc gcgagcggcg tcagcaccgg
     1501 gaagaccgta tcgtcgaagg ttcgctccag cgccgtctgc gtttcgtcgt ccaaatcgga
     1561 gacccgcatc agccggatgc cctcgcgctc cagcgccggc agcaagtcgt cgttgagcag
     1621 ccgcatctgg gtgtgcagcg aagtgcgcag ccgctccgag atcgccgaaa gatgctcggc
     1681 cggcatgcgg ccgtcgtcgg aacgccgcac cacctgggcc tcgatctgct gcttgatcgc
     1741 cgcgacccgg atcatgaaga actcgtcgag gttggtcccg tagatcgcga cgaatttcaa
     1801 gcgttctagc agcgggttgc gcggatcgag cgcctcttcg agaacccggt cgttgaactc
     1861 cagccaggac agctcgcggc tgatgtagag cgagggatcg tccacgggga ccggggagag
     1921 gcggggctcg ggcaggacgg acggcttaat catgcgttgg ctcaaccgct ttcgccgccg
     1981 tcggtcaaaa cactagggga ctctcgaaga agtcggcgac cgcgccaggc acggaacggg
     2041 agcggggccg aacccttggt gaccgatgaa caccgcgcag ccgaagggct cgtagcagca
     2101 tgtggagcga tttggcggcg cacgcgggcc agcacgtcgt gttggccctc accgcgctcg
     2161 gat
//
LOCUS       DASUCJ010001357         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1222015, whole genome shotgun sequence.
ACCESSION   DASUCJ010001357 DASUCJ010000000
VERSION     DASUCJ010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222015"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFU90_15785"
     CDS             complement(<1..1271)
                     /locus_tag="VFU90_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481307.1"
                     /translation="MTRRRLVAIASAGLLLFIGVVIAGVLLALTQTDMGRAWVRTYVQ
                     QTLATALHGNGSMYIGRLGGNLLSGFTIDSLSIRDAEDSSFVVSGPVKLHYDLRDLVD
                     KRIRLSSVDLERPVINLRRHADHSWNFQHIFPGGPPAKPGARRVPRPQELRFGDYITM
                     SSVTIHDGTVILTEPWDAPDSLRGARRDSAIRVALRDTSRIIRRTSEGVKQTRLFTHL
                     ELSAPDVRIADPDSSGRYVQVASLDVDISDPPFRVRHATGPVTIRGDSVWLSMRHFDL
                     PGSTGSATAKVWWGGDLPTRYDVHVTADSVSLADVAWVYPTLPRTGGGRMRLTIHNEL
                     NLHVLDYVLSDMDVRTTRSRLQGTMTFAVGGPVLGVKDLAVTVSPLDFDLLRQFNGGP
                     FPVDWQGQFTGSVRARGGPLTRFDVDDAHLE"
     gene            complement(1273..>1547)
                     /locus_tag="VFU90_15790"
     CDS             complement(1273..>1547)
                     /locus_tag="VFU90_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481308.1"
                     /translation="VRLTPGIGVRVNSFVGPFRVDVAYNPYQSSLGSVYFVDQSSDAL
                     RCVSPHNPFDRGVVVPGVDCPSTFRPVQTGSFFSRLTFNFSIGQAF"
BASE COUNT          292 a          548 c          485 g          222 t
ORIGIN      
        1 aattcgagat gggcatcgtc gacatcgaac cgggtgagcg gaccgccgcg cgcgcgcacc
       61 gatcccgtga actgcccctg ccaatccacc ggaaacggac cgccgttgaa ctgccgcaag
      121 agatcgaagt cgagcggcga cacggtgacc gccagatcct tcacgcccag caccggcccg
      181 cccaccgcaa aggtcatcgt gccctggagc cgcgaccgcg tcgtgcgcac gtccatgtcg
      241 ctcagcacgt aatccagcac gtgcaggttg agctcgttgt ggatcgttag gcgcatgcgc
      301 ccgccgccgg tgcgcggcag cgtcggatat acccacgcca catccgccag cgagacggaa
      361 tccgccgtca cgtggacgtc gtatcgcgtt ggaagatcgc cgccccacca cactttggcg
      421 gtggcggatc ccgtggatcc cggcaggtcg aagtggcgca tcgacagcca caccgagtcg
      481 ccgcgaatcg tcaccggccc cgttgcatga cgcacacgga acggcggatc actgatgtcg
      541 acgtcgagcg acgccacctg cacgtaccgc ccgctgctgt ccggatccgc aatgcgcacg
      601 tccggcgccg acaactcgag atgcgtgaac aggcgcgttt gcttgacgcc ctcgctcgtg
      661 cggcgaatga tgcgcgacgt atcgcgcagc gccacgcgga tcgcgctgtc gcgccgtgcg
      721 ccgcgcagcg agtccggcgc gtcccacggc tcggtgagga tcaccgtccc gtcgtggatc
      781 gtcaccgacg acatcgtgat gtagtcgccg aaccgcagct cttgcggccg gggcacccgc
      841 cgtgcgccgg gcttggccgg cggcccgccc gggaaaatgt gctggaagtt ccaggagtga
      901 tctgcgtgcc gccgcagatt gatcacgggc cgctcgagat ccacggacga caaccggatc
      961 cgcttgtcca ccagatcgcg caggtcgtag tgcagcttga ccggccccga caccacgaac
     1021 gacgaatcct cggcatcgcg gatggacaac gagtcgatcg tgaaaccgct caggagattc
     1081 ccgcctaacc ggccgatgta catcgagccg ttgccgtgca gcgccgtcgc cagcgtctgc
     1141 tgcacgtagg tgcgcaccca cgcgcggccc atgtcggtct gcgtgagcgc gagcagcacg
     1201 ccggcgatca ccacgccgat gaacagcagc aagcccgcgc tcgcgatcgc gaccaaccgg
     1261 cggcgcgtca tgttagaacg cctgaccgat gctgaagttg aacgtcagcc ggctgaagaa
     1321 cgaacccgtt tgcaccggac ggaacgtcga cgggcagtcc acgcccggca ccaccacgcc
     1381 gcgatcgaat ggattgtgcg gactcacgca ccgcagcgcg tcgctcgact ggtccacgaa
     1441 atacacggag cccagcgacg actgatacgg attgtacgcc acgtccaccc ggaacggccc
     1501 gacgaacgag ttgacgcgca ccccgatccc aggcgttagg cgcacat
//
LOCUS       DASUCJ010001358         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_213476, whole genome shotgun sequence.
ACCESSION   DASUCJ010001358 DASUCJ010000000
VERSION     DASUCJ010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213476"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFU90_15795"
     CDS             complement(<1..422)
                     /locus_tag="VFU90_15795"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481309.1"
                     /translation="MPDAPGRLLVEPIVRAALLEDVGRGGDLTTEAIVPASLRARARI
                     VAREPGIIAGTDAAALAFALLDESVEVRVAIGDGRRVVSGEAVAEVTGNARAILTAER
                     TALNLLCRLSGIATATRELVELVAGTRARIADTRKTTPG"
     gene            complement(415..1944)
                     /locus_tag="VFU90_15800"
     CDS             complement(415..1944)
                     /locus_tag="VFU90_15800"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU5481310.1"
                     /translation="MERRQSGVLVVGAGIAGLMAALKLAPVRVTVLCKTRLGKGAATD
                     WAQGGIAAAIGKDDSPRLHAIDTQRAGAGINDEKIVAILTQDAPARIEELLELGAAFD
                     RDAAGELALGREAAHQRRRIVKAGGDATGHAILRTLIEAVTNHPSIEVVEEVSAADLI
                     VEGERVYGVYGTRADGSEATAFLGDAVVLATGGIGRLYRYTTNPVQATGDGIAMAARG
                     GALLADMEFVQFHPTALAIGRDPMPLVTEAVRGEGAVLVNDLGERFMLDLHPDAELAP
                     RDVVARAIFEQLQRGRTVGLDARTAIGDRFPREFPTVFGFCMDAGIDPRVQNIPVAPA
                     AHYHMGGIAVDEWGRASLRRLWACGETSATGVHGANRLASNSLLEALVYGSRVAADIK
                     NAAAVAGSPEGLSYDTGAHGDGREAQAISDLRNVMYANVGLVRDERGLREALLRIAEL
                     ETAFPKARNDLRNLLVVGRLIAEAALARHESRGSHYRSDYAHTDTALAKRSFTRLRTL
                     A"
     gene            complement(1955..2983)
                     /gene="nadA"
                     /locus_tag="VFU90_15805"
     CDS             complement(1955..2983)
                     /gene="nadA"
                     /locus_tag="VFU90_15805"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU5481311.1"
                     /translation="MSVLDLPYTPDVAAETDPIFERVAHVIPRVEWPLHAPYVAAINR
                     LKRERNAVILAHNYQVPEIFYTVADIVGDSLALAMKAAETDADVIVLCGVHFMAETAK
                     LVNPSKTVLVPDLKAGCSLAESITGADVRLLRERYPGVPIVTYVNTSAEVKAESDVCV
                     TSGNALQIVEALGVPRVVFLPDEYLAKYVASKTGVEIVAWKGHCEVHERFTGSDVRRF
                     REDDPSLVVIAHPECPPDVLAEADFVGSTQGMTEYVGSLSAAQPAARVLLMTECSMAD
                     NVSGRYPSVEFVRPCNLCPHMKRNTLPKVLHSLEAMEFEVTIDPNVAERARRSVQRML
                     EFSRQPRD"
     gene            3174..3722
                     /locus_tag="VFU90_15810"
     CDS             3174..3722
                     /locus_tag="VFU90_15810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5481312.1"
                     /translation="MAQVGKQAPEFALPSTKDATSAKNLGKTVSLSDYRGKWLVFFFY
                     PLDFTFVCPTEITALSDRAEEFAEFDAEILGCSTDSVFSHWAWLGTPRERNGIAGTKY
                     PLAADFTKEVARAYGVLDEATGAAQRGLFIVDPDGILKYAVVTDDSVGRSVDETLRVL
                     QALQTGGLCPANWKPGKDLLAV"
     gene            3722..4195
                     /locus_tag="VFU90_15815"
     CDS             3722..4195
                     /locus_tag="VFU90_15815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5481313.1"
                     /translation="MPLRMRSPLPSLDGATAWVNREPSASELRGRAVVVSFWSTSCHL
                     CHEGAELAARWRDRFADRGVAFVAVHQPRSAEELDVESVRRDATGGMQLTQPVAIDNA
                     HAIVDRFGNEFVPAYYVFDRGHELRHFQAGGKGLERIEAAIERVLAEPAEAPASA"
BASE COUNT          753 a         1577 c         1674 g          865 t
ORIGIN      
        1 ccgggcgtgg tttttcgcgt atcggcgatc cgcgcgcgcg ttcccgcgac gagctcgacc
       61 agctcgcgcg tcgccgtcgc gattcccgaa agccggcaca gcaggttgag cgcggttcgc
      121 tcggcggtga ggatggcgcg cgcgttgccg gtgacttcgg cgaccgcttc gcccgaaacc
      181 acgcgacggc cgtcgccgat cgcgacgcgc acctcgaccg attcgtcgag cagtgcgaag
      241 gccagcgccg cggcatccgt cccggcgatg atgccgggct ctctcgcgac gattcgagcc
      301 cgcgcgcgca ggctcgctgg aacgatcgct tcggtcgtaa ggtcgccgcc gcgtccgacg
      361 tcctcgagca gcgccgcgcg aacgatgggc tcgacgagga ggcggccggg cgcgtcaggc
      421 aagggtgcga agccgggtga acgagcgttt tgcgagcgcc gtatcggtgt gcgcgtagtc
      481 gctgcggtaa tggctgccgc ggctttcgtg ccgcgcgagc gccgcttcgg cgatcagccg
      541 tccgaccacc agcaggttgc gcaaatcgtt tcgcgccttt ggaaacgccg tttccagttc
      601 ggcgatgcgg agcagcgctt cgcgcaagcc gcgctcgtcg cgcaccaagc cgacgttggc
      661 gtacatgacg ttgcgcaagt cgctgatcgc ctgggcttcg cggccgtccc cgtgcgcgcc
      721 ggtatcgtac gacaagcctt cggggcttcc ggcaacggcg gcagcgtttt tgatgtcggc
      781 ggcgacgcgc gagccgtaca ccagcgcttc gagcaacgag ttgctcgcga gccggttggc
      841 gccgtgcact ccggtcgcgc tcgtctcgcc gcaggcccac aaacgccgca gcgacgcgcg
      901 gccccattcg tcgacggcga taccgcccat gtgatagtgg gccgcgggag cgacggggat
      961 gttttgtacg cgcggatcga ttccggcatc catgcagaat ccgaacacgg tgggaaactc
     1021 gcgcgggaaa cggtcgccga ttgccgtccg ggcgtcgagg ccgacggtgc gcccgcgttg
     1081 gagctgttcg aagatggcgc gcgccacgac gtcgcggggt gcgagctcgg cgtcggggtg
     1141 gagatcgagc atgaaccgtt cgccgagatc gttgacgagc accgcgcctt cgccgcgaac
     1201 ggcctcggta acgagcggca tcgggtcgcg cccgatcgcc agcgcggttg gatggaactg
     1261 cacgaactcc atgtccgcga gcagcgcgcc gccgcgcgcc gccatcgcga ttccgtctcc
     1321 ggtggcctgc acggggttgg tcgtgtagcg gtacaggcgt ccgatcccgc cggtcgcgag
     1381 tacgaccgcg tcgccgagaa acgcagtcgc ttccgaaccg tcggcgcgcg taccgtacac
     1441 gccgtagacg cgctctccct cgacgatcag gtcggcggcc gacacttctt cgaccacctc
     1501 gatgctgggg tgattcgtca ccgcctcgat caaggtgcgc aggatcgcgt gtccggtggc
     1561 gtcgccgccc gccttgacga tacggcgccg ctgatgcgcc gcctcgcgcc ccaaggccag
     1621 ttcgccggcc gcatcgcggt cgaaggcggc gcccagctcg agtaattctt cgatgcgcgc
     1681 cggcgcgtct tgcgtgagga tggcgacgat tttctcgtcg ttgatgccgg cgccggcgcg
     1741 ctgcgtgtcg atcgcgtgca aacgcggtga gtcgtccttg ccgatcgcgg ccgcgatccc
     1801 gccctgcgcc cagtcggtcg ccgcgccctt ccccaggcgc gtcttgcaca gaacggtgac
     1861 gcgcaccggt gcgagtttga gggcggccat caggccggcg atgccggcgc cgacgaccaa
     1921 gactccgctt tgcctccgtt ccatcggttg cgtgtcagtc gcgcggctgg cggctgaact
     1981 cgagcatccg ctgcaccgat cggcgcgcgc gttcggccac gttcgggtcg atcgtcacct
     2041 cgaactccat cgcttcgagg ctgtgcagga ccttcggcaa ggtattgcgt ttcatgtgcg
     2101 ggcagagatt gcagggccgg acgaactcga ccgatggata gcgccccgaa acgttgtcgg
     2161 ccatcgagca ctcggtcatc agcaacacgc gagcggccgg ctgcgccgcg ctcaagctgc
     2221 cgacgtactc cgtcatgccc tgcgtcgagc cgacgaaatc ggcttcggcg agcacgtcgg
     2281 gagggcactc cggatgcgcg attacgacta acgacggatc gtcttcgcgg aaccggcgga
     2341 cgtcgctgcc ggtgaaccgt tcgtgaactt cgcagtggcc cttccaggcg acgatctcga
     2401 cgccggtctt cgaggcgacg tacttggcga ggtactcgtc gggcaagaaa acgactcgcg
     2461 ggacgccgag agcttccacg atttgtaagg cgttgccgct ggtcacgcaa acgtccgact
     2521 cggccttcac ttcggccgac gtgttgacgt aagtaacgat cggcacgccc ggatagcgtt
     2581 cgcgcaagag ccggacgtcg gccccggtaa tcgattcggc cagcgagcat ccggctttaa
     2641 ggtccggcac gagcaccgtc ttcgacggat tgacgagctt ggcggtttcc gccatgaagt
     2701 gcacgccgca cagcacgatc acgtcggcgt cggtctcggc ggccttcatc gctagcgcga
     2761 gcgaatcgcc cacgatgtcg gcgaccgtat agaagatctc cggtacttgg tagttgtgcg
     2821 cgaggatgac ggcgttgcgt tcccgtttga gccggttgat cgcagcgacg tacggagcgt
     2881 ggagcggcca ttcgacgcgc ggaatcacgt gcgccacgcg ctcgaagatc ggatcggttt
     2941 cggcggcgac gtcgggggtg taggggagat cgagaacgct catcggctcg tctttggttg
     3001 cggttgcggc gcggggtccc ctgcgcgccg ccagggctat aagatgaaag tcgttttcac
     3061 tacatgggga gtttgggtac ggtactccgg ggcgccgcgg gcgcgcatcc cgcgctgccg
     3121 ctcccctgga agtcgctcgt ttgctggaaa gtaaggttga ggttcgatcg atgttggctc
     3181 aggttggtaa gcaagctccc gagttcgcgc tccccagtac gaaggatgcg acgtcggcaa
     3241 aaaacctcgg aaagacggta tcgctttcgg actaccgcgg aaagtggctg gtgtttttct
     3301 tctacccgct ggacttcacg ttcgtgtgtc cgaccgagat caccgcactc tccgaccgtg
     3361 ccgaagagtt cgcggaattc gacgccgaga ttctcggctg ctcgaccgac tcggtgttca
     3421 gccactgggc atggctgggc acgccgcgcg agcgcaacgg catcgccggc acgaagtacc
     3481 cgctggccgc ggatttcacc aaggaagtcg cgcgcgcgta cggcgtgctc gacgaagcca
     3541 ccggcgcggc gcaacgcggt ttgttcatcg tcgatcccga cggcatcctg aagtacgcgg
     3601 tcgtgaccga cgatagcgtc ggccgcagcg tcgacgagac cttacgcgta ctgcaggcac
     3661 tccagaccgg cggcctttgc ccggcgaact ggaagcccgg caaggatctc ctggccgtct
     3721 aatgccgctt cggatgcgca gtccgctccc ctcgctggac ggtgcgacgg cgtgggtcaa
     3781 tcgcgagccg tcggcgtccg agctgcgcgg acgcgcggtc gtcgtttcgt tttggtcgac
     3841 gagctgtcac ctctgtcacg agggtgcgga actggctgcg cggtggcgcg accgttttgc
     3901 cgatcgcggc gtcgccttcg tcgcggtgca ccagccgcgt tccgcggagg agctcgacgt
     3961 cgagtccgtg cgccgcgacg cgaccggcgg gatgcagctg acgcagccgg tggcgatcga
     4021 caacgcccac gcgatcgtcg accgcttcgg caacgagttc gttccggcgt actacgtgtt
     4081 cgaccgcggc cacgagttgc ggcatttcca agccggcggc aagggcctcg agcgaatcga
     4141 agcggcgatc gagcgcgtgc tggccgaacc ggccgaagcg cccgcttcgg cttaagggaa
     4201 cctcgaacgg cgtcgccccg gctggccgaa acacgctacg cgggcgcgcg tacgtgtggt
     4261 ggaggcaatc cgatgcagac ccgaatcgta gtattcgccc tggcggccgg gctgacggcc
     4321 gtggtgctcg cgctcgcgtc gctggccgtg tgaccggaat tccggccaaa ccgagcgggg
     4381 gacaggtgcg tgacttgccc ggccttcggt ggtaggagtg cggaagagga tcttctcgct
     4441 tcaccgatag gaaggaactc ccatgaatcg atggtttgcc ggcgtcgttt tgtccgccgt
     4501 cgtggcgctg ggggttggag ccctcggcgc ggccgcgcag ccggctccgt caccctcgcc
     4561 tagcgcatct ccgacacctg cggcgacgat gcagccgtag gctcacttgc ggtaccgcgc
     4621 ggcggtgccg ggccttccgg atgcggcgcc cgatcgggcg ccgcatcctt ttttgccgcc
     4681 ggaaggggat aaccgccggc cggaagaacg gggacgcgcg ccatgaagat gactttagtt
     4741 attattcttg gttcaccttg gaaaataacc aaagtctttt aatatggcga gctacgatgg
     4801 ggggtctttc cgaaaatgca agcgatcttt aaggccggag cggcgattgc ggccctcgcc
     4861 gtggcgctg
//
LOCUS       DASUCJ010001359         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1313470, whole genome shotgun sequence.
ACCESSION   DASUCJ010001359 DASUCJ010000000
VERSION     DASUCJ010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313470"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VFU90_15820"
     CDS             complement(<1..1251)
                     /locus_tag="VFU90_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481314.1"
                     /translation="MNVWALVDLPQSNPSGSYSVTLDSGIPAVNYVVPGGVRGGFGVA
                     GPGRVAAQTGFPLRLFWGSPGGFNAVMPGTRYYGAVLIDPLNGGSYAALGQAGFVPFA
                     LTRSAGNDDVADALETDATRTVSLAPGETLAHQFIDVASQATLLVDTSYVTGSPNRGA
                     VNFRLTRADFPAPSASPQIASAPAATSSDAQWSLGGTTTGKSVTVPVKAGRWYFVASN
                     ASANNADVALTVKTNLIQSVPPVVAGAYYNPQRSGHGTFMNQAGGEQALYWYTYLEDG
                     TPVWYVAQAPAAGSGVWTAPLGRITWNGATVGQVAIVGDVTLTPVDANGHMFSWHLFG
                     QSGSERFVLLGADQCVPFNGAQADFTGQWYAPTQSGYGMDVLAIPDGSAGASFQFDAF
                     YFYDALGQPRWAAGSSSPFGSQSTL"
BASE COUNT          219 a          429 c          422 g          195 t
ORIGIN      
        1 caatgtcgat tgcgaaccga acggcgacga cgagcctgcc gcccagcgcg gctggccgag
       61 cgcgtcgtag aaatagaacg catcgaactg gaagctcgcc ccggcggaac cgtccggtat
      121 cgccagcacg tccatgccgt aaccggattg cgtcggcgcg taccattggc cggtgaaatc
      181 ggcctgcgcg ccgttaaatg gcacgcactg gtcggcgcca agcagcacga aacgttcgct
      241 gccggactgt ccgaacagat gccaggaaaa catgtggccg ttcgcatcga cgggcgtcaa
      301 tgtcacgtcg ccgacgatgg cgacctgccc gacggtcgcg ccattccagg ttatgcgccc
      361 cagcggcgcg gtccatacgc cgctgccggc ggccggggcc tgcgccacgt accacaccgg
      421 cgtgccgtct tcgagatagg tgtaccagta cagcgcttgc tcgccgcccg cttgattcat
      481 gaaggtgcca tgtccggatc gctgcgggtt gtaataggca ccggcgacga cgggcggcac
      541 ggactggatc aaattcgtct tcaccgtgag cgcaacatca gcattgttcg cgctcgcatt
      601 ggacgcgacg aagtaccagc gcccggcctt gaccggcacg gtgacggatt tgcccgtcgt
      661 cgtgccgccc agcgaccatt gcgcatcgct ggaagttgcg gcaggcgcgc tggcgatctg
      721 cggcgaggcc gacggcgccg ggaagtcggc gcgggtcagg cggaaattca ccgcgccgcg
      781 attcggcgat ccggtgacgt agctcgtatc gacgagcagc gttgcctggc tggcgacgtc
      841 gatgaattgg tgcgcgagcg tttcgccggg ggccagcgac acggtgcgcg tcgcatccgt
      901 ttccagcgca tcggcgacgt cgtcgttgcc cgcgctgcgg gtcagcgcga atggcacgaa
      961 gccggcctgg cccaacgccg cgtagcttcc cccgttcaac ggatcgatca gtacggcgcc
     1021 gtagtagcgc gtgccgggca tgaccgcatt gaagccgccg ggcgaacccc agaacaggcg
     1081 cagcggaaag cccgtctgcg cggcgacgcg acccggcccg gccaccccga aaccgccgcg
     1141 cacgccgccc ggcacgacgt agttcaccgc cgggatgccg ctgtcgagcg ttaccgagta
     1201 gctgcccgac ggattgctct gcggaagatc gaccagtgcc cacacgttca ccggcccggc
     1261 attgc
//
LOCUS       DASUCJ010001360         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_350775, whole genome shotgun sequence.
ACCESSION   DASUCJ010001360 DASUCJ010000000
VERSION     DASUCJ010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350775"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1181
                     /locus_tag="VFU90_15825"
     CDS             15..1181
                     /locus_tag="VFU90_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEU5481315.1"
                     /translation="MGTPATRAQIIEGLIQEKYVLREGRELAPTAKAFSLITLLHGLG
                     VPELFSPELTAEWEFNLAQMEHGKLKREQFMRDIVRMTKHIVKQAKSHESDTIPGDFA
                     TLSARCPKCGGEIHENYKKFQCQACDFGFWKIIAGRQLDVPEAETLLTERGVGPLEGF
                     RSKQGRPFATRLKLTEDYEVQFDFGDGGDGGSAAEAPDFTGQEPLGECPKCGGRVFEL
                     PATYVCERSVGPERTCDFRSGRVILQRPIEREQVKKLLATGRTDLLQFVSARTRRPFA
                     AFLVRQPDGKVGFEFEAKDRNKAGARRSRGAAALRVLGPHPKDKQPVELHAGRYGPYV
                     KHGAINATLPDKDRVDAVTLGEAVELLAAKAKAAKVATPKAKAASRTGQRKRAA"
     gene            complement(1198..>2029)
                     /locus_tag="VFU90_15830"
     CDS             complement(1198..>2029)
                     /locus_tag="VFU90_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5481316.1"
                     /translation="PRVALVSFTGSSAVGRQIAQTVAGRFGRTLLECSGNNGIIVAED
                     ADLDLVIPAVLFGAVGTAGQRCTTTRRLIVHESIEPEVVRRLREAYAQVRIGDPLDSA
                     TLMGPLIDAPAVANFRKAVDEAIASGGKMVCGGKVLDRPGFFVEPTIIRGVRNDWPVV
                     QRETFAPILYVMSYRDLPEAIAMHNAVPQGLSSSIFTGSVRNAEAFLSGSGSDCGIAN
                     VNIGTSGAEIGGAFGGEKETGGGREAGSDAWQAYMRRQTTTINWSRELPLAQGIKFGA
                     "
BASE COUNT          349 a          694 c          682 g          304 t
ORIGIN      
        1 tgcgcgacaa gggcctggga acgccggcca cgcgggcgca gatcatcgaa gggctgatcc
       61 aggaaaagta cgtcctgcgc gagggacggg agctcgcccc gaccgccaag gcgttctccc
      121 tcatcacgct gctgcacggc ctcggcgtac cggagctctt ctcgcccgaa ctcaccgcgg
      181 agtgggagtt caacctcgcg cagatggagc acggcaagct caagcgcgag cagttcatgc
      241 gcgacatcgt ccgcatgacc aagcacatcg tcaagcaggc aaagagccac gaaagcgaca
      301 cgatccccgg cgacttcgcc acgctctcgg cgcgctgccc gaagtgtggc ggcgagattc
      361 acgagaacta caagaagttc cagtgtcaag cgtgcgattt cggcttctgg aagatcatcg
      421 ccgggcgtca gctcgacgtt cccgaggccg agacgctgct taccgagcgc ggcgtcggcc
      481 cgctcgaagg cttcaggagc aagcagggac gtcctttcgc gacgcggctc aagctcaccg
      541 aagactacga agtgcagttc gacttcggcg acggcggcga tggcgggtcc gccgcggagg
      601 cgccggactt caccgggcag gagccgctgg gcgagtgtcc caagtgcggc ggccgcgtat
      661 tcgaattgcc cgccacgtac gtatgcgagc gttcggtcgg acccgagcgc acgtgcgatt
      721 tccgctccgg acgagtgatc ctgcagcggc cgatcgagcg cgagcaggtg aaaaagctcc
      781 ttgctacggg ccggaccgat ctcctgcagt tcgtctcggc gcgcacccgc aggccgtttg
      841 ccgccttcct cgtccgccag ccggatggca aggtcggttt tgagttcgag gccaaggatc
      901 gcaacaaggc cggggcacgt cgtagccgcg gcgctgcggc gttgcgcgtg ctgggcccgc
      961 atccgaagga caagcaaccg gtggagctgc acgccgggcg ttacggccct tatgtgaagc
     1021 acggggccat caacgccacg ctgcccgaca aggatcgcgt ggacgccgtc acgctgggcg
     1081 aagcggtcga gttgctcgct gccaaggcga aagcggcgaa ggttgcgacc ccaaaagcca
     1141 aggccgcgtc caggaccggc cagcgcaagc gcgcagcgta accggctcgg ccgcgctcta
     1201 ggcgccgaac ttgatgcctt gcgcgagcgg cagctcgcga ctccagttga tggtcgtcgt
     1261 ctgacggcgc atgtaggctt gccaggcgtc cgacccggcc tcgcgcccgc cgccggtctc
     1321 cttttcgccg ccgaacgcgc cgccgatctc ggcgcccgac gtgccgatgt tcacgttggc
     1381 gatgccgcaa tcgctgcccg accccgagag gaacgcctcg gcgttgcgga cgcttccggt
     1441 gaagatcgac gacgacaggc cctgcggcac cgcgttgtgc atcgcgatcg cctcggggag
     1501 atcacggtac gacataacgt agaggatcgg cgcgaaggtc tcccgctgca cgaccggcca
     1561 gtcgttgcgt acgccgcgga tgatggtcgg ctcgacgaag aacccgggtc ggtcgagtac
     1621 cttgccaccg cacaccatct ttccgcccga ggcgatcgcc tcgtccaccg ccttgcggaa
     1681 gttcgcgacc gccggggcgt cgatcagcgg ccccatcagc gtcgcggagt cgaggggatc
     1741 gccgatgcgc acctgggcgt acgcctcgcg caggcgccgt acgacttccg gctcgatcga
     1801 ttcgtgcacg atcagccgcc gcgtcgtcgt gcagcgctgg ccggccgtgc cgaccgcccc
     1861 gaacagcacc gccggaatca ccagatcgag gtccgcgtct tcggcgacga tgatgccgtt
     1921 gttgcccgag cactcgagca gcgtgcgccc gaatcggccc gctaccgtct gcgcgatctg
     1981 gcgccccacg gccgacgagc cggtgaagga gacgagcgct acgcgcggg
//
LOCUS       DASUCJ010001361         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_350786, whole genome shotgun sequence.
ACCESSION   DASUCJ010001361 DASUCJ010000000
VERSION     DASUCJ010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350786"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFU90_15835"
     CDS             <1..891
                     /locus_tag="VFU90_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481317.1"
                     /translation="FDLVFNMCEGLDGVAALEPAVISALEVLAVPFTGCSSWTASLCL
                     RKHVVNAFLERAGLPVPAFAVVHRGAPVPAVGFPAICKPAAEDASVGVEQRSVVRTNR
                     ALAARVEAMHEGWDEVLVQRYIDGREVNVGIVGNTVLPLSEIRFDGLPKRMWRIVSYR
                     SKWVAGSDEDVGAVPACPADLSDTISDKVKEVALDAWRLVGGTGYGRVDMRIDHEGQP
                     WILEVNANPDIAADAGLARMAAAAGLDYAALIRTVCDDAMTRHVSNGDDRWALAQRLS
                     GVMPEANDAAALQLVAVGQR"
     gene            1232..2404
                     /locus_tag="VFU90_15840"
     CDS             1232..2404
                     /locus_tag="VFU90_15840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU5481318.1"
                     /translation="MSEWQRILKEDSIATLEHLADRFGRDVIDVEALKPAFDNFQMRI
                     TPAALEQITAVGDPMWNQYVPTVQELEVHDGIIDSLDEDADSPVPNITHRYPDRVLFL
                     VSPVCASYCRFCTRRRKVGDPEKIPLNQYESAFAYIRAHPEIRDIVMSGGDPMMLSDR
                     RLEFLLQNLRAIPHVEIIRIGSRVTSHLPERITPEFCEMVKKYHPVYMNTHFNHPREL
                     TPKVVEALGRLANAGVPLGCQTVLLRGVNDDVAVMKELMQRLLKARVRPYCIYMADQV
                     AGGEHFRTTVQKGLEIVRALRGWTSGLAVPHFVIDSPGGGGKVPLLPEYVEEITDDEV
                     VFRNYEGKRFVYKQPHLPVPAAACASDADSPDIVPIEIGKTPRRKQPARRRKSANG"
     gene            complement(2434..>2628)
                     /locus_tag="VFU90_15845"
     CDS             complement(2434..>2628)
                     /locus_tag="VFU90_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481319.1"
                     /translation="DGLGTIVKAVVPQAEMHLYATHLNSMTHGYATFTRRFRGYEEVP
                     GEAAQKVIADAAKPEELVEA"
BASE COUNT          446 a          879 c          833 g          470 t
ORIGIN      
        1 ttcgacctcg tgttcaacat gtgcgaagga ctcgacggcg tcgccgcact cgagccggcc
       61 gtcatctcgg cgctcgaagt gttggccgtg ccgttcaccg gttgctcgtc gtggaccgcg
      121 agcctgtgtc tccgcaagca cgtcgtcaac gcgttcctcg agcgggccgg attgcccgtg
      181 cctgctttcg ccgtggtgca tcgcggcgcg cccgtacccg ctgtcgggtt tccagccatc
      241 tgcaagcccg ctgcagaaga cgcatcggtc ggcgtcgagc agcgctcggt tgttcgcacc
      301 aatcgtgcgc tcgccgcgcg cgtcgaggcg atgcacgaag gctgggacga agtgctcgtc
      361 caacgataca tcgacggtcg cgaagtcaac gtcggcatcg tcggcaatac cgtgcttccg
      421 ctctccgaga tccggttcga tggactgccg aagcgaatgt ggcggatcgt ttcgtatcga
      481 tcgaagtggg tcgccggcag cgatgaagat gtcggcgccg tgcccgcctg tcccgcggat
      541 ctctcggaca cgatcagcga caaggtcaaa gaagtcgcgc tcgatgcgtg gcgactcgtc
      601 ggcggcaccg gctacggccg cgtcgacatg cgcatcgatc acgaaggaca gccgtggatt
      661 ctcgaggtga acgcgaatcc cgacatcgcg gccgatgccg gactcgcgcg catggcggcc
      721 gccgccggcc tcgactacgc cgcgctcatc cggaccgtct gcgacgacgc gatgacgcgc
      781 cacgtgtcga acggcgacga tcgttgggca ttggcccaac gcttgtccgg cgtgatgccg
      841 gaggccaatg acgccgccgc gctgcagctc gtcgccgtcg gtcaacgctg atccatggac
      901 gttccccgag gtagatggga ctcgatgcac gcggctcggc gtcattcgcc agtcgcgcgt
      961 caggcatcga gtcctctcct ctcacgactc gctcctcggc gccttcaccc cggaatccga
     1021 gtcggcaaca ttcccacagc gccgtcgctg taccggctgc gcgtcgcatg tgcgacgccc
     1081 cgaggcatcg tccggtcgcg acgcgtcctc cctccccgag gtcgatcgaa ggcttcaccc
     1141 agcgtgcgcc agcaggtccc gtctttcatc gaccccgacg taacaccacg atcatctcac
     1201 gctcgcctat caggcgggga gcccaatcac catgagtgag tggcaacgca tcctgaagga
     1261 agacagcatc gccaccctgg agcatttggc cgaccggttc ggtcgtgacg tcatcgacgt
     1321 agaggcgctc aaaccggcct tcgacaactt ccagatgcgc atcacgccgg ccgcgctcga
     1381 gcagatcact gccgtcggcg acccgatgtg gaaccagtac gttcccacgg tgcaggagct
     1441 cgaggtccac gacggcatca tcgattcgct cgacgaggat gccgattcgc cggtgcctaa
     1501 cattacgcac cgctatcccg atcgcgtcct cttcctcgtc agtccggtgt gtgcgtcgta
     1561 ctgccggttc tgtacgcgcc gccgcaaggt gggcgacccg gaaaagattc cgctcaatca
     1621 atacgagtcg gcgttcgcgt acatccgggc gcatcccgag attcgcgaca tcgtgatgag
     1681 cggcggcgat ccgatgatgc tttcggaccg ccggctcgaa ttcctgttgc agaatctccg
     1741 cgccattccg cacgtggaga tcatccgcat cgggagccgc gtcacgtcgc acctgccgga
     1801 gcgcatcacg ccggagttct gcgagatggt gaagaagtac catccggtct acatgaacac
     1861 gcacttcaac cacccgcgcg agttgacgcc gaaggtggtc gaggccctgg gccgcctggc
     1921 gaacgccggc gttccgttag gctgccagac cgtcctgctg cggggcgtga acgatgatgt
     1981 ggccgtgatg aaggagctga tgcagcgcct gctcaaggcg cgcgtgcggc cgtactgtat
     2041 ctacatggcc gaccaggtgg cgggcggcga acatttccgc acgacggtgc agaaagggtt
     2101 ggagatcgtg cgcgccctgc gcggatggac gtccgggttg gcggtgccgc acttcgtgat
     2161 cgattcgccc ggcggcggcg gcaaagtgcc gctgctcccc gagtatgtcg aagagatcac
     2221 cgacgacgaa gtcgtattcc ggaattacga gggcaagcgg ttcgtgtaca agcagccgca
     2281 tcttccggtt ccagcggccg cttgcgcgtc cgacgccgat tcgcccgaca tcgtgccgat
     2341 cgagatcggc aaaacgccgc gccggaagca gccggcgcgg cgcaggaagt cggccaacgg
     2401 atgaccgttt gcgtaaggcg cggcggccgc gccttacgct tcgaccagct cttccggctt
     2461 ggcggcgtcg gcaatgacct tctgcgccgc ctcgcccgga acctcctcat agccgcggaa
     2521 gcgacgggtg aacgtcgcat agccgtgcgt catcgagttg aggtgtgtcg cgtagagatg
     2581 catctcggcc tgcggcacca ccgctttcac gatcgtgcct aacccgtc
//
LOCUS       DASUCJ010001362         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1053797, whole genome shotgun sequence.
ACCESSION   DASUCJ010001362 DASUCJ010000000
VERSION     DASUCJ010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053797"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFU90_15850"
     CDS             <1..341
                     /locus_tag="VFU90_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481320.1"
                     /translation="SAAALAFPVAWGSFRPYGGVGFSMNLIQQVGQIDQPADPLVEPQ
                     IQDQLQNNRDRISFIALAGLQASYRRYSLFGQVTYMPSAAKFLLNGRSTYILEGGIRY
                     NFGSSIDRPE"
     gene            complement(478..>1288)
                     /locus_tag="VFU90_15855"
     CDS             complement(478..>1288)
                     /locus_tag="VFU90_15855"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5481321.1"
                     /translation="GADIRRAKADGVVAILIGVEGGHAIEDSLDHLRELKRRGACYLT
                     LTWNNGNHWAGSSIGSGATRTAGLTPFGRQVIAELNRLGMLVDVSHTSSETLADVLAA
                     SEAPVIASHSSARALADHPRNLSDEELRAIAARGGVICVNFYSRFIEVRMRDAMDAAE
                     ARAEAEPAGTAAQRSARLEALLARIPPTALSILIDHIDHIARVAGIDHVGLGSDFDGI
                     RLTPASLDDVTAYPRIAQALLDRGYSDDDVRKVLGANVLRLIDTVVTAIPR"
BASE COUNT          227 a          375 c          468 g          218 t
ORIGIN      
        1 acagcgcggc ggcgttggcg tttccggtgg cgtggggaag cttccgtccg tatggcggcg
       61 tcgggttttc gatgaatctg atccagcaag ttggacagat cgatcagccc gcggatccgc
      121 tggtcgagcc gcaaattcag gatcagctgc agaacaaccg cgacagaatt tccttcatcg
      181 cgctcgccgg tctgcaggcg tcgtatcgcc ggtactcgtt gttcggccag gtgacctaca
      241 tgccgtcggc ggcgaaattc ctgttgaatg gccggtcgac gtacatattg gagggcggaa
      301 tccggtacaa cttcgggtcg tcgatcgata gaccggagtg aaagacggac actgccgaag
      361 taaaagacgg acactgccgg acaaaagacg gacaaagcgc ggacaacttt aagacggcca
      421 ctgccggata acagacggac aaagcgcgga caagacaagg cgggcgaggg gcgttcatca
      481 gcgcgggatc gcggtcacta cggtgtcgat gagacgaagg acgttggcgc cgaggacttt
      541 gcggacatcg tcgtcgctgt agccgcgatc gaggagtgct tgcgcgatgc gcgggtaggc
      601 cgtgacgtcg tcgaggctgg ctggcgttag gcggatacca tcgaagtcgc tgccgagtcc
      661 gacgtggtcg atgccggcga cgcgggcgat gtggtcgatg tggtcgatga ggatcgagag
      721 cgccgtgggc ggaatgcgcg ccagcagcgc ctcgaggcga gcggaccgtt gcgcggccgt
      781 gccggccggc tcggcttcgg ctcgcgcttc ggcggcatcc atcgcgtcgc gcatgcgcac
      841 ctcgatgaag cgcgagtaga agttgacgca gatcacgccg ccgcgcgcgg cgatggcgcg
      901 cagctcctcg tcgctgagat tccgcggatg gtcggcgagc gcccgggcgc tcgagtggga
      961 cgcgatgacc ggcgcctccg acgcggcgag gacatcggcg agcgtttcgc tcgacgtgtg
     1021 cgacacgtcg acgagcatgc ctaaccgatt gagctcggcg atcacctggc ggccgaacgg
     1081 cgtgaggccg gcggtgcgcg tggctccgga gccgatcgac gatcccgccc agtggtttcc
     1141 gttgttccac gtgagggtga ggtagcatgc gccgcggcgc ttgagctccc ggagatggtc
     1201 gagcgaatct tcgatcgcgt gtccgccttc gacgccgatc aggatcgcga cgacgccgtc
     1261 ggccttggcg cgccggatgt cggcgcca
//
LOCUS       DASUCJ010001363         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1115141, whole genome shotgun sequence.
ACCESSION   DASUCJ010001363 DASUCJ010000000
VERSION     DASUCJ010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115141"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VFU90_15860"
     CDS             complement(<1..1047)
                     /locus_tag="VFU90_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5481322.1"
                     /translation="MSKSCLRTFAAVAAVALLCVPALKPLAAGADETTLTLHDAARSE
                     ATSPEDALALARRRLDVHDADAAVAGLQRYLSVYPQELTVARFLADLYLRMGALTNAE
                     AAYRDIVDRYPYDDEAHRRLGTLDLLENRVDPAIAEFEAALPDSIDDLVVAHERKGDL
                     EAFERSMEQRASSRSGDAQVQYEMAETYDDLRLPHDALTYFERAEAIYPKSTDILNGL
                     AIAQMQVGDDAAAERTFARCLTVDSEDYACTNDLGSLYLDRKRYADARRELLRAHQLA
                     PEGPEALVNLGYLSDELGNRPAAVAYYAEAIYVWPYFADAYVNLSFDQIRQGSIDQAE
                     GVLRKGLSIAPKDPR"
     gene            complement(1056..>1269)
                     /locus_tag="VFU90_15865"
     CDS             complement(1056..>1269)
                     /locus_tag="VFU90_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481323.1"
                     /translation="AEVGTARLPPDHPRWVRDAVAASARARELVGKLKPVVVAVMSLW
                     DHRLRSIIVGGRRLRVDPSGSYRADA"
BASE COUNT          210 a          420 c          426 g          213 t
ORIGIN      
        1 ccgcgggtcc ttcggcgcga tcgacaaccc tttgcgcagg acgccttccg cctgatcgat
       61 cgagccttgt cggatttgat cgaagcttag attcacgtac gcgtccgcga aatacggcca
      121 cacgtagatc gcttcggcgt aatacgccac cgccgccggg cgatttccca gttcgtcgga
      181 gaggtagccg aggttcacca gcgcctcggg cccctcgggg gcgagttgat gcgcccgcag
      241 cagctcgcgc cgcgcgtcgg catagcgttt gcgatcgaga tagagcgatc cgagatcgtt
      301 ggtacacgcg tagtcttcgg aatcgacggt gagacagcgc gcgaaggtcc gttcggccgc
      361 agcgtcgtcc ccgacttgca tttgcgcgat cgccaaaccg ttgaggatgt cggtcgactt
      421 cggataaatc gcttccgccc gttcgaagta cgtcagcgcg tcgtgcggca gccgcagatc
      481 gtcgtacgtc tcggccatct cgtattgcac ctgtgcgtcg ccggagcgcg agctcgcgcg
      541 ctgctccatc gatcgctcga acgcctcgag gtcgcccttg cgctcgtggg ccactaccag
      601 atcgtcgatc gagtcgggga gggcggcttc gaactcggcg atcgccggat ccacgcgatt
      661 ttccagcagg tcgagcgttc cgagacggcg gtgcgcctcg tcgtcgtacg ggtaacggtc
      721 gacgatgtcg cgatacgccg cctccgcgtt ggtgagggcg cccatccgca gatacagatc
      781 ggcgagaaag cgcgcgacgg taagctcctg cgggtacacg ctcaagtaac gctgcagccc
      841 cgcgaccgcg gcatcggcgt cgtggacgtc gaggcgccgg cgggcgagcg cgagggcgtc
      901 ttccggactg gtggcctcgg agcgggccgc atcgtgcagg gtgagcgtcg tctcgtcggc
      961 accggccgca agcggcttca acgccggcac gcacagcagc gcgaccgctg cgacggcggc
     1021 gaaagtgcga agacacgatt ttgacaaatt tcgaatcacg cgtccgcccg gtaactgccg
     1081 ctcggatcga cccggagccg gcggcctcca acgatgatag aacgtaagcg gtggtcccag
     1141 agtgacatca cggcgacgac caccggtttg agcttgccca ccagttcgcg ggcgcgggcc
     1201 gacgcggcga cggcgtcgcg cacccaccgg ggatgatccg gcggcaggcg cgccgtcccc
     1261 acctcggcc
//
LOCUS       DASUCJ010001364         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_901212, whole genome shotgun sequence.
ACCESSION   DASUCJ010001364 DASUCJ010000000
VERSION     DASUCJ010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901212"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1286
                     /locus_tag="VFU90_15870"
     CDS             150..1286
                     /locus_tag="VFU90_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481324.1"
                     /translation="MKAGRWAFSFLFFAAAAFADSGEHAGPITFSTLVTLPGGIEGLT
                     SDYQGNLYTPMRATAPAPCPVLKIPVNDPTHYVVVGNIPAPGATTSCSPSGLAFGPDG
                     QLYVTQSDNNIYRFTPNETTPPTATVFASGVPGNNGLAFDWNGNLWTGDGTTGFGRVW
                     RISPDGATVIEEFRIPPMANGATVGTGVGRSVLTLPTGNAQPLVANGVQFDHAHNLYI
                     ADTARGALWKVHLGSDSTPLVRIGCDSTLPADTLCFDHVWAQNPLMEGADGFVLDVAG
                     NAWVDANERNAVVFVVNGTRQVIEAFRNPPDATTHLRNTGPLETPTSPVLMGHQLCTA
                     NSDGNRRDNSPSSAGEIGGSGGPKGKISCMDQRVAIPGMVLPIH"
BASE COUNT          223 a          471 c          419 g          192 t
ORIGIN      
        1 ccgcagctca ccgtcgtgcc gaaggccgag tagggaataa accgcggttt cctcgatgcg
       61 gaacggcgtt cgcctcgctt gcatgcgcgt atcgcgcgga gcaggataag cgcgcttcgc
      121 caatcccatg acaccgagga gaaatcgcca tgaaagcggg acggtgggct ttttccttcc
      181 tgttcttcgc ggccgcggcg ttcgccgatt ccggcgagca cgcgggcccc atcacgttct
      241 cgacgctcgt cacgctgccc ggcggcatcg aaggcctcac gagcgactat caaggcaacc
      301 tatacacgcc gatgcgagcg acggcgcccg cgccatgccc ggtgctcaag atccccgtca
      361 acgacccgac gcattacgtc gtggtcggca acatcccggc ccccggcgcc accacgtcgt
      421 gctcgcccag cggcctcgct ttcggtcccg acgggcagct ctacgtgacg cagtcggaca
      481 acaacatcta tcgcttcacg ccgaacgaga cgacgccgcc tacggccacc gttttcgcga
      541 gcggcgtgcc gggcaacaac ggcctcgcct tcgactggaa cggcaacctg tggaccggtg
      601 acggaacgac gggcttcggg cgcgtgtggc ggatctcgcc ggacggggcg acggtgatcg
      661 aggaattccg catcccgccg atggcgaacg gcgccaccgt cggcaccggc gtcgggcgca
      721 gcgtcctcac gctgcccacc ggcaatgccc agccgctcgt cgcgaacggc gtgcagttcg
      781 accacgcgca caacctgtac atcgccgata ccgcgcgcgg cgcgctctgg aaggtgcacc
      841 tcggctccga cagcacgccc ctggtgcgga tcggctgcga ctccacgctc cccgcggata
      901 cgctctgctt cgaccacgtc tgggcgcaga acccgctcat ggaaggcgcg gacggcttcg
      961 tgctcgacgt ggcgggcaac gcgtgggtcg acgccaacga gcgcaatgcg gtggtcttcg
     1021 tcgtgaacgg cacgcgccag gtgatcgagg cattccgcaa cccgccggac gccacgacgc
     1081 acctgcgcaa cacgggaccg ctggagacgc cgaccagccc ggtgctcatg ggccatcagc
     1141 tgtgcaccgc gaactccgac ggcaaccgtc gcgacaactc gccttcgagc gccggcgaga
     1201 tcggcggcag cggcgggccc aagggcaaga tctcgtgcat ggaccagcgc gtggcgatcc
     1261 cgggcatggt gctgccgatc cactgatcgt cggatcgatt ccccg
//
LOCUS       DASUCJ010001365         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_457874, whole genome shotgun sequence.
ACCESSION   DASUCJ010001365 DASUCJ010000000
VERSION     DASUCJ010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457874"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFU90_15875"
     CDS             <1..207
                     /locus_tag="VFU90_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481325.1"
                     /translation="EVAVAIGRHFAALTGDGTHWPSGWRATLELPDGARYIDVLTGRD
                     GTFSERAELSNLFSEIPVCALLRQ"
     gene            375..1775
                     /locus_tag="VFU90_15880"
     CDS             375..1775
                     /locus_tag="VFU90_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5481326.1"
                     /translation="MGGLASALLPAVRKSGVVWFGSSGKLRETADGSPPLVQLETYGR
                     GTIATVDLPAQHYAGFYEGFANSGLWPLLHSRPDLARTNSHDYAAYSEVNAYMARTLA
                     AFAGDDATIWVHDYHFLPLGAELRELGIAREIGFFLHTPWPNRRVMAKLPQYRDLVHA
                     MLAYDLIGFQTDRDRDNFAELLRTDMHLPGSGLTFRTHRGVCRLATHPIGIDARDFAA
                     SADRSAQDAEVQRLRHSIGGAKLIIGVDRLDYSKGLVQRIHAFNRLLSQNPDLSRKLS
                     MLQIAVPSRSTIDAYRDLHKEVAAVVGEVNGRHGEVDWTPLRYLNKGYPQSTLAGFYR
                     TSAVGLVTPIFDGMNLVAKEYVAAQNPEDPGVLVLSRYAGAANELDAALLVDPQNIEG
                     VSRQLQTALAMPRSERRARWQHMNDRLLRHSIHRWFADFMQDLKTPRPNVLPLTGTAA
                     PALRPSVQDRALVAHS"
BASE COUNT          306 a          630 c          553 g          343 t
ORIGIN      
        1 gaagtggctg ttgccattgg ccgtcacttc gccgcgctca ccggtgacgg tacgcattgg
       61 ccgtcgggat ggcgcgcgac gctcgagttg ccggatggtg cgcgctacat cgacgtgctc
      121 acgggccgcg atggaacttt ctcggagcgg gcggaacttt ctaatttgtt ttcggaaatt
      181 ccggtctgcg ccctgctgcg tcagtagcaa cttccttttt ttcgctcgtt catcccattc
      241 atcaaggaac ttcgacgcct ccgttcggtt gtcaaagcag cgccggcacg cgctttttcg
      301 acaattcggg gagaggtttc ttggacatcg ttgtttccaa tcgggtggcg cgggttacgc
      361 ccggtggacc aatgatgggc gggctcgcat ccgcgctgtt gcccgccgtg cgcaaatccg
      421 gcgtcgtctg gttcggctcg agcggaaaat tacgcgagac ggcagacggt tcgcccccgc
      481 tcgtgcaact tgaaacctat ggacgcggca cgatcgcaac cgtcgacctg cccgcgcagc
      541 attacgccgg cttctacgaa ggattcgcca attcgggcct atggccgctg ctgcattcgc
      601 ggccggatct cgccagaacc aattcgcacg actacgccgc ttacagcgaa gtgaacgcct
      661 acatggcgcg gacgcttgcc gccttcgccg gcgacgacgc caccatctgg gtgcacgact
      721 accatttcct gccgctcggc gccgagcttc gcgaactcgg catcgcccgc gagatcggtt
      781 ttttcctgca cacgccgtgg ccgaaccgcc gcgtcatggc gaagctgccg caatatcgtg
      841 atctcgtgca cgcgatgctc gcctacgacc tcatcggctt ccagaccgac cgcgaccgcg
      901 acaatttcgc ggaactgctc cgcaccgata tgcatctgcc tggctccggt ctgacgttcc
      961 ggacgcaccg gggggtctgc cgcctcgcca cgcacccgat cggcatcgat gcccgcgact
     1021 tcgcggcgag cgcggatcgg tcggctcagg acgccgaggt gcaacgcctg cgccacagca
     1081 tcggcggcgc gaagctgatc atcggcgtcg accgcctcga ttattcgaaa ggcctggttc
     1141 agcgcattca cgcgttcaac cgtctgctgt cgcagaaccc ggacctgtcg cgcaaactct
     1201 caatgcttca gatcgcggtg ccgtcgcgca gcaccatcga cgcctatcgc gacctgcaca
     1261 aggaagtcgc cgcggtcgtc ggcgaggtga acggccgtca cggcgaggtc gactggacgc
     1321 ccctgcgcta tctcaacaaa ggctacccgc aatcgacgct cgcgggtttc taccggacgt
     1381 ccgcagtcgg cctggttacg ccgatcttcg acggcatgaa tctcgtcgca aaggaatacg
     1441 ttgcggcgca gaacccggag gatcccggcg tcctcgtcct gtcgcgctac gccggcgccg
     1501 ccaacgaact cgacgccgcg ctgctggtcg acccgcagaa catcgaggga gtgagccggc
     1561 agttgcagac cgccttggcc atgccgcgca gcgagcgacg cgcccgctgg cagcacatga
     1621 acgatcggct cctgcgccac tcgatccacc gctggttcgc ggacttcatg caggacctga
     1681 aaacgccgcg gccgaacgtg ctgccgctca ccgggaccgc cgccccggcg ctgcgcccct
     1741 cggtgcagga tcgcgcgctc gtcgcgcatt cctgatcgca cgacatggcc gcaatcggac
     1801 atcgtcgttg ggctatcgcc gagggctaca tt
//
LOCUS       DASUCJ010001366         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1390309, whole genome shotgun sequence.
ACCESSION   DASUCJ010001366 DASUCJ010000000
VERSION     DASUCJ010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390309"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFU90_15885"
     CDS             <1..357
                     /locus_tag="VFU90_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481327.1"
                     /translation="AYPATLTDRGWWNVTGLAWSGRGRITRVDVSADGGKTWAPAVLE
                     QPVLSKCHTRFSFPWKWDGREATLMSRATDETGYVQPTLAQLRAARGTGTRYHFNNIR
                     AWRIAPDGSVTFGLDG"
     gene            354..938
                     /locus_tag="VFU90_15890"
     CDS             354..938
                     /locus_tag="VFU90_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5481328.1"
                     /translation="MTRAAMLRAALLAVAAVSPTLVRRAEPRSVPPARYGLGRPATAD
                     EIRAWDVSVMPDGTGLPVGSGTVVQGATIFVQKCARCHGPTGVEGPFDRLVGREPKQG
                     FPFGRDDSLVRTIGNYWPYSTTVYDYIHRAMPYDAPGSLEPNEVYSVVAFLLWRNEII
                     ADTAVMNARTLPRVRMPARDRFVVDNRRGGPEVR"
BASE COUNT          178 a          359 c          390 g          172 t
ORIGIN      
        1 gcgtatccgg ccacgctgac ggatcgcggt tggtggaacg tgaccggtct cgcgtggagc
       61 ggccggggac ggatcacgcg cgtcgatgtg tcggcagatg gcggaaaaac gtgggcgccc
      121 gcggtgctcg agcagcccgt actctccaag tgccacacgc ggttctcgtt tccgtggaag
      181 tgggacggac gcgaggccac gctcatgagc cgcgcaaccg atgagaccgg atacgtccaa
      241 ccgacgctgg cgcaactgcg cgccgcgaga gggaccggca cgcgctatca cttcaacaac
      301 attcgcgcgt ggcgtatcgc accggacggc agcgtgacct ttggactcga cggatgacga
      361 gagccgcgat gctgagggcg gcgctcctcg cggtggcggc ggtctcgccc actctcgtta
      421 ggcgagccga gcctcgctct gtccctccgg cgcgctacgg gctcggccgc cccgccacgg
      481 ccgatgagat ccgcgcctgg gacgtgagcg tgatgccgga cggcacggga ttgccggtcg
      541 gtagcgggac cgtcgtccaa ggcgcgacga tcttcgttca gaagtgcgca cgctgtcatg
      601 gcccaacggg agtcgagggc ccgttcgacc ggctcgtcgg ccgggagccg aaacagggct
      661 tcccgttcgg ccgcgacgac tcgctcgtgc gtaccattgg gaactattgg ccgtattcga
      721 cgaccgtgta cgactacatt catcgggcca tgccctacga cgcaccgggc tcgctcgagc
      781 cgaacgaggt gtacagcgtc gtcgcgtttc tgctctggcg caacgagatc atcgcggaca
      841 cggcggtcat gaacgcgcgc acgctgcccc gcgtgcgcat gcccgcgcgc gatcggtttg
      901 tggtggacaa tcgtcgcggc gggccagagg ttcggtagtg acgggcacga catcaccgaa
      961 atccgagaga ctcgcgtgcc ggtaatgcga cgcattggca cgttggccac gtgcgccgcc
     1021 gagggcgggc agggcgacat ccatccgatc accaacgcgg cgctcgcgtg gcgcgacggg
     1081 cgcatcgcgt gggtcggcc
//
LOCUS       DASUCJ010001367         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_671705, whole genome shotgun sequence.
ACCESSION   DASUCJ010001367 DASUCJ010000000
VERSION     DASUCJ010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671705"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1195)
                     /locus_tag="VFU90_15895"
     CDS             complement(320..1195)
                     /locus_tag="VFU90_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5481329.1"
                     /translation="MNLRQSAEIQSEPDYLDVTAPDGVRLSVRTGGRRGAPAIVFIHG
                     FNQSHGAWAKQFEGPLADEFHLVAFDLRGHGDSDKPEDEAAYRESKRWADDVAAILRA
                     LDLTRVVLVGWSYGGRVILDYLAAYGPHALAGINFVAGVVGDEKEYYGEDVGLIKGTY
                     SSNPAEAAEATRKFLRACFADEPPAADFEQMLAYNMAVTPDVRKKLGGRKVGAHDLLA
                     GLTLPVLFTHGTHDRIIAPAMSEYGAATVPNARLSIFEGTGHSPFYEKHGLFDSELRD
                     FVHACHTIPEPAEEE"
     gene            1321..>1686
                     /locus_tag="VFU90_15900"
     CDS             1321..>1686
                     /locus_tag="VFU90_15900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5481330.1"
                     /translation="MQPSVEILPFDAQRESFEELTRLVNGAYRQLGEMGLNYVAVDQG
                     AEVTRARVESASACWVARRGGKLAGTICYYATSRDRSSPAWYRREEVCHFGQFAVDPP
                     EQRSGIGELLMEAAEARALA"
BASE COUNT          248 a          503 c          615 g          320 t
ORIGIN      
        1 ggtaccctgg tcgcaacctt tcttcgtgcc ggtcaggccg aggtcgtcgc ggagtgcgtc
       61 gagcagcgtg acccgcggtt cgacttcgag cgtgcgttcg cggccgttga ccgtgagggt
      121 aagcttggtg gtgcgcgttt cgctaatcat ggggcgtcct tcgactgcgc gcctgcggcg
      181 ctccgctcag cccttcgact gcgcgtgctt cgcacgctcc gctcaggatg acacacatag
      241 cgctccgctc aggatgacac acatagcgct ccgctcagga tgacacacat agcgctccgc
      301 tcgtgatggc gcgtggcggt tattcttcct cggcgggttc ggggatggtg tggcaggcgt
      361 gcacgaagtc gcggagttcg ctgtcgaaca atccgtgctt ttcgtagaac ggcgagtgac
      421 cggtcccttc gaagatcgag agccgagcgt tcggcacggt ggcggcgccg tattcgctca
      481 tcgcgggcgc gatgatgcga tcgtgcgtgc cgtgggtgaa gagcacgggg agcgtgaggc
      541 cggctagcag atcgtgcgcg ccgactttgc gtccgccgag tttctttcgg acatcgggcg
      601 tgacggccat gttgtaggca agcatctgct cgaaatcggc ggccggcggc tcgtcggcga
      661 agcaggcgcg caggaatttg cgggtcgctt cggcggcttc cgcggggttg ctcgagtacg
      721 ttcctttgat gagcccgacg tcttcgccgt agtactcttt ctcgtcgccg acgacgccgg
      781 cgacgaagtt gatgccggcc agcgcgtgcg gtccgtacgc ggcgagataa tcgaggatga
      841 cgcgtccgcc gtacgaccag ccgacgagca cgacgcgggt gaggtcgagc gcgcgcagaa
      901 tcgcggcgac gtcgtcggcc cagcgtttgg attcgcggta ggcggcctcg tcttcgggct
      961 tgtcggaatc gccgtggccg cgcaaatcga aggcgacgag gtggaactcg tcggccagcg
     1021 ggccttcgaa ctgcttggcc cacgcgccgt ggctctgatt gaagccgtgg atgaagacga
     1081 tcgccggcgc gccgcggcgg ccgccggtgc gcacgctcag gcgcacgccg tcgggcgcgg
     1141 tgacgtcgag atagtccggt tcgctctgga tttcagccga ctgtctaagg ttcactcccc
     1201 gctccttgga aagactgccg gtggggcttc ttcgcggtgg gcggggcgcg gtcattgcgg
     1261 cgtccgaatc ggggcgccgg acgcgagggc ttcgcacgct gcgcgcgaca gtagagcggc
     1321 atgcagccga gcgttgagat actgccgttt gacgcgcagc gcgagtcgtt cgaggagctt
     1381 actcggttgg tcaacggcgc gtatcgccaa cttggcgaga tgggtctcaa ctacgtcgcc
     1441 gtcgatcaag gcgccgaggt gacccgcgct cgcgtggagt ctgcatcggc gtgctgggtg
     1501 gcgcggagag gcggcaaact tgccggcacg atttgctact acgcgacgtc gcgcgaccgg
     1561 agttcaccag cgtggtatcg gcgcgaagag gtttgccatt tcggacagtt tgcggtcgat
     1621 ccgccggagc agcggtccgg gatcggcgag ctgctgatgg aagcggcgga agcgcgcgcg
     1681 ctcgcg
//
LOCUS       DASUCJ010001368         4629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1435714, whole genome shotgun sequence.
ACCESSION   DASUCJ010001368 DASUCJ010000000
VERSION     DASUCJ010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435714"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFU90_15905"
     CDS             <1..960
                     /locus_tag="VFU90_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481331.1"
                     /translation="ATARSHVVGIVTGAFAAALPLGAGSAAAHGFAGARFFPATIATD
                     DPFVADELSLPTLSWQKNPGDATGAPATREFDVSVDFARRITANFGIEASATWKTLRT
                     PGSPTVRGFDNVSLGAKYQVFESDAHEAIVSAGVDWDVGHSGAKRVGAESISTVTPTL
                     FFGKGWGDLPHSMAWLRPVAVTGLVGVGIPSRASTTTIADDGTVDVERHPHTLQWGIV
                     FEYSIPYLNANVQATGWNDAFNRLIPVVEFAMSTALDRGSSATTGTVNPGLLWAGRYV
                     QLAAEAAIPVNRAGGSSVGWTLQLHLFLDDLAPKAFGRPLFGG"
     gene            962..1327
                     /gene="copC"
                     /locus_tag="VFU90_15910"
     CDS             962..1327
                     /gene="copC"
                     /locus_tag="VFU90_15910"
                     /inference="COORDINATES: protein motif:HMM:NF033814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEU5481332.1"
                     /translation="MADLRALAVALLVSVMLAPEAFAHAFLDSATPAVGSAVHGAPVQ
                     VELHFSEGLEPAFSTVRVDDAKGKRVDAGDVHVDPSDASILEVSLGKLAPGRYHVTWR
                     VVSIDTHVTEGAFTFDVLP"
     gene            1341..2639
                     /gene="copD"
                     /locus_tag="VFU90_15915"
     CDS             1341..2639
                     /gene="copD"
                     /locus_tag="VFU90_15915"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEU5481333.1"
                     /translation="METAFALLRAVHEIAALIALGQVVYSRLISRTFTERARSVVIES
                     LAISVVAALGWLLCEAANMSGLPLFEALSPSVISVVLRKTLFGRIWVLRLILGMALCG
                     WIVVLHRKAATATPTAQSSLLSLLALYVATLALVGHAVAAQGVEQVVRIAADAAHLLA
                     AGAWLGALPALAYDLAHVRRNAGSLRFDAASAATRRFSMLGIACVGTLIATGTVNAWY
                     LVGSRLALIGTPYGHWLLAKLALFAAMIALAAHNRQRLAPRIGAHEPAAPSRLARNAS
                     IEFALGAGVIAIVGHLGITVPAIHDRPMIPALLASGVSGAGFATGRGASPIPLTDDVV
                     ARGRASYLIHCVECHGTQGRGDGPRAASLPVHPADLAAHGSMHSEADLHAMIANGIPG
                     TPMPAFWARLSDREIWELVQYVRALAGVPVPRATPATHHQ"
     gene            complement(2892..3731)
                     /locus_tag="VFU90_15920"
     CDS             complement(2892..3731)
                     /locus_tag="VFU90_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481334.1"
                     /translation="MTRARTQITLVLGSDEVASGIARALHRAGYRVVLTDEIDPPCPL
                     RGLSYVGAWYFGAAEPGGVGACFCSSIKSVPEIIDRQRLVATTAWSVEGLSRALSPAL
                     LVDARAANGNAAGPLRGVPGKPLIVVGGPASGRGDEAAVAIDPSFGSTAGRPIRLHTS
                     AIDLHLPWRLDGADANGWVVAARDGRFHTELRVGADVAEESVIGTIQGAPLRAGIAGR
                     VVALTLRGARVPAGQLVAVIDEEPAISRLAALSRRADRIANGVISVLASQGSRLDVVE
                     SIT"
     gene            4230..>4629
                     /locus_tag="VFU90_15925"
     CDS             4230..>4629
                     /locus_tag="VFU90_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5481335.1"
                     /translation="MMNALAKIAAVATLAAASAASAAGITGAGSTFAYPIFARWSATY
                     HATGGPEVNYQSIGSGAGIAQIKAKTVDFGASDMPLTVADLDKAGLVQFPAIMGGVVP
                     VVNLEGVAPGALKLSGTLLADIYLGSVKSWN"
BASE COUNT          718 a         1549 c         1545 g          817 t
ORIGIN      
        1 gccaccgcgc gcagtcatgt cgtggggatc gttacgggcg cgttcgcggc tgcgcttccg
       61 ctcggcgctg gatccgccgc agcccatggt tttgccgggg cccgcttctt ccccgcgacg
      121 atcgcgaccg acgacccatt cgttgccgac gagctctcac tgccgacgct gtcctggcag
      181 aagaatccgg gggacgccac gggtgcgccg gccacgcggg aattcgatgt ctcggtcgat
      241 ttcgcgcgac ggatcaccgc gaatttcggc atcgaggcga gcgccacctg gaagaccctt
      301 cggactccgg gttcgccgac cgtgcgcggc ttcgacaacg tcagcctcgg cgcgaaatat
      361 caggtcttcg aaagcgatgc gcacgaggcg atcgtgtcgg ccggggtcga ctgggacgtc
      421 ggccactcgg gcgcgaaacg ggtcggcgct gaatcgatca gcaccgtcac gccaacgctc
      481 ttcttcggca agggttgggg cgatcttccc cattcgatgg cgtggctgcg cccggtcgcc
      541 gtgaccggcc tcgtcggcgt cggcatcccc tcacgcgcga gcacgaccac gatcgccgac
      601 gacggtacgg tcgacgtcga gcgtcacccc catacgctcc aatgggggat tgtgttcgag
      661 tacagcattc cctacctcaa tgcgaacgtg caggcgacgg gctggaacga cgcgttcaac
      721 cggctgatcc cggtggtgga gttcgcgatg tcgaccgcac tcgatcgtgg ctcgagcgcg
      781 accaccggaa cggtgaaccc tgggctgctc tgggcggggc gctacgtgca gctcgcggcc
      841 gaggcggcga ttccggtaaa ccgcgccggc gggtcatctg tcggctggac gctgcagctt
      901 cacctgttcc tcgacgatct cgccccgaag gcgttcgggc gaccactgtt cgggggatga
      961 aatggccgat ctacgcgctc tcgcggtcgc gctgctggtg tcggtcatgt tggcgccaga
     1021 ggcgtttgcc cacgcgttcc tcgattcagc gacccctgcc gtcggaagtg ccgtccacgg
     1081 cgcgccagtg caagtcgaac ttcacttcag cgaagggttg gaacccgcct tcagcacggt
     1141 ccgtgtcgac gacgcgaagg gcaagcgtgt cgacgccggc gacgttcacg tcgatcccag
     1201 cgatgcgtcg attctcgagg tatcgcttgg aaaactcgca ccggggcgct accacgtgac
     1261 gtggcgcgtg gtgtcgatcg atacccatgt gaccgagggc gcgttcacct tcgacgtgct
     1321 tccgtagcgg cggggcggcg ctggagacgg cattcgcact ccttcgcgcg gttcacgaga
     1381 tcgccgcgct gatcgcgctc gggcaggtcg tctattcccg gctgatcagc cgtacgttca
     1441 ccgagcgagc gcggtcggtg gtcatcgaga gcctcgcgat cagcgtcgtc gccgcgctcg
     1501 gctggctcct ttgcgaagcc gcgaacatga gcgggctgcc gcttttcgag gcgctctcgc
     1561 cctcggtgat ctcggtcgtg ctgaggaaga cgttgtttgg gcgcatatgg gtgctgcgcc
     1621 tcatcctcgg catggcgctt tgcggatgga tcgtggtgct gcaccgcaag gcggccacgg
     1681 ccacgcccac cgcgcagtca tcgttactca gcctgctcgc tctctatgtg gcaacgcttg
     1741 cgcttgtcgg gcacgcggtc gccgcacagg gtgtcgagca ggtggtgcgc atcgccgccg
     1801 atgcggcgca cctgctcgcc gcgggtgcct ggctcggtgc gcttcccgcc cttgcctacg
     1861 acctggccca cgtacggcga aatgccggct cgctacggtt cgacgccgcc tccgccgcga
     1921 cgaggcgctt ctcgatgctg ggcatcgcgt gcgtcggcac cctgatcgca accgggaccg
     1981 tgaatgcatg gtatctcgtc ggaagccggc tcgcgcttat cgggaccccg tatggccact
     2041 ggctgctcgc gaagctcgca ttgttcgcag cgatgatcgc gctggctgcg cacaatcgcc
     2101 agcgcctcgc accacggatt ggcgcacacg agcccgcggc gccttcgcgg ctggcgcgta
     2161 acgcgagcat cgaattcgcg ctcggcgccg gcgtgatcgc gattgtcggc caccttggaa
     2221 ttaccgttcc ggcgattcac gatcgcccga tgattcctgc gttgctcgct tccggcgtga
     2281 gcggggcggg ctttgcgacc ggtcgtggag cctcgccgat accccttacc gacgacgtcg
     2341 tcgcgcgcgg tcgagcgagc tatctcatcc actgtgtcga gtgccacggg actcagggtc
     2401 gcggcgacgg cccgcgggcc gcgtcgcttc ctgtccatcc ggccgacctg gcggcgcacg
     2461 gctcgatgca ctcggaggcc gatctccacg cgatgatcgc caacggcatt cccggcacac
     2521 cgatgcccgc cttctgggcg cgcctctccg accgtgagat ctgggagctc gtccagtacg
     2581 ttcgcgcact ggctggcgtt ccagttcccc gggcgacgcc agcgacgcat catcaataac
     2641 cgcggctgcc gaagagacaa cgcttattac cgcgctgaag tcccggcgcc agcctcccgt
     2701 taccagattc gctccatcag aggcgtctgg aggagtgggg gaattccagg ggtttgttcg
     2761 atgacgctgc gatcggcgcc gcggcgcgcg agcgacagat gctcacgctg cgggtgaact
     2821 ccaccgtcga cggcggaaag acgccgggtt gggccgggcg gttcgcgaag gggttcgaat
     2881 ggtgccatcg ttcaggtgat cgattcaacg acatcgaggc gggacccctg cgacgcgagg
     2941 acgctgatca ccccattggc gattcgatcg gcacggcgcg agagcgcggc aaggcgagag
     3001 atcgccggct cttcgtcgat caccgcaacg agctgacctg cgggcacacg cgcaccacgc
     3061 aacgtgaggg ccaccacgcg gcccgcgatt ccggcgcgca gtggggctcc ctggatcgtt
     3121 ccgatcaccg attcctccgc gacgtcggcg ccaacgcgca gttccgtgtg aaaccggcca
     3181 tcgcgagctg ccacgaccca cccgttcgcg tcggcgccgt cgagtcgcca gggtaggtga
     3241 agatcgatcg ccgacgtatg caagcgaatc ggccgtccgg cagtcgaccc gaacgacgga
     3301 tcgatcgcga cggcggcttc gtccccgcga ccggatgccg gaccaccaac cactatgagc
     3361 ggctttccgg gaacgccgcg cagcggaccg gcagcgtttc cgttggcagc ccgcgcgtcg
     3421 acgagcaagg ccggcgagag tgcacgggaa agtccttcca cggaccacgc ggtagtcgcg
     3481 acaagtctct ggcggtcgat aatctccggc acgctcttga tcgacgagca gaagcacgcg
     3541 ccgacgccgc caggctccgc cgccccgaag taccaggcac cgacgtacga cagcccgcgg
     3601 agcgggcagg gcgggtcgat ctcgtcggtg agcaccacgc ggtacccggc gcgatgcagc
     3661 gcccgcgcga tacccgaagc gacttcgtcc gatccgagga cgagcgtgat ttgcgtgcgc
     3721 gcacgggtca ttgcgcggaa caggtcgcgc agatcggtcg cgtacggcgc gactcggggc
     3781 cttgcgctcg cccggatgca atgcccggct tcttctgcgg ccgactcgcg agcgcctgtt
     3841 cgatcggcgc cggcacgtgt acctgtcgat tcaggtagag cgcctgccag gcccacgcac
     3901 gtttctgcgc tgctctcgac actttcgacg tcacgatcac gatgcgcccc ttcctgtacg
     3961 cgcccctgcg cgcttggtcg gccgatgggc ggggtggcat ttgttacggc gtcgaccgga
     4021 tcgataggga ccggtgccgc gcacgtcttc gttccacccc atgcgggcga agactagcga
     4081 ccgaatgtga catgcatgtg tcaggcgcgt cgttctttcg cggcagtttg ttgcgcaccg
     4141 tcgctcgagg tggtgtcatc gttctgtcac atccgcattg gacaatgccg gcgtcccgga
     4201 gttaacccgg ggatacggag aatgaattca tgatgaatgc attggcaaag atcgcagcgg
     4261 tggcgacgct ggctgctgcg tctgcagcga gcgctgcagg aatcaccggc gcaggctcga
     4321 ccttcgcgta tccgatcttc gcgcgctggt cggcaaccta ccacgccacg ggcggccccg
     4381 aggtcaacta tcagtcgatc ggatcgggcg cggggatcgc gcaaatcaag gcgaagacgg
     4441 tcgacttcgg agcctccgac atgccgctca cggtcgccga tctcgacaag gcgggcctgg
     4501 tccagtttcc ggcgatcatg ggcggcgtgg taccggtcgt gaacctcgaa ggtgttgcgc
     4561 cgggcgcgct caagctgagc gggacgctgc tcgccgacat ttacctcggt agcgtcaaga
     4621 gctggaacg
//
LOCUS       DASUCJ010001369         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_931621, whole genome shotgun sequence.
ACCESSION   DASUCJ010001369 DASUCJ010000000
VERSION     DASUCJ010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931621"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..227)
                     /locus_tag="VFU90_15930"
     CDS             complement(6..227)
                     /locus_tag="VFU90_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481336.1"
                     /translation="MAHPALGQAVGVVVTPKNGVALEAADIVAACRATLPAYMVPAVV
                     DVREGPLPRNPNGKIDRRLLATQFESTQA"
     gene            complement(328..>1103)
                     /locus_tag="VFU90_15935"
     CDS             complement(328..>1103)
                     /locus_tag="VFU90_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5481337.1"
                     /translation="LRYFANTGGRMPLETLKALRARVPRAKPFLMYGLTEAFRSTYLP
                     PEEVDRRPDSIGNAIPNAEILVLRPDGTRCQPNEPGELVHRGALVGLGYWNDPEKTAE
                     RYRPLPGREAGLVLPEIAVFSGDTVRMDDEGFLYFVGRRDEMIKTSGYRVSPTEIEEA
                     LYATGRVGEAAAFGVPHPSLGQAIVVVATPRNGLALEAHDLVAHCREMLPAYMVPAIV
                     DVRTGLLPRNPNGKIDRKAIGAEFEHRFENATQRAEPRS"
BASE COUNT          174 a          368 c          375 g          186 t
ORIGIN      
        1 gatcgtcatg cctgcgtcga ttcgaattgc gtcgcgagga gcctgcggtc gatcttgccg
       61 ttcggattcc gcggcagcgg accttcgcgt acgtcgacga cggcgggcac catgtacgcc
      121 ggcagcgtgg cacggcaggc ggcgacgatg tccgcggctt cgagcgcaac cccgttcttc
      181 ggcgtcacca cgacgccgac cgcctgaccg agtgctggat gcgccacgca gacgcaggcc
      241 ccgcctcggc ggcgatccgc gcgagctcgg gatcgctgaa gcgatggcac ccgcgaacca
      301 tcgccgtctc gtcgagcttc gggggcctca tgatcgaggc tccgcgcgct gcgtcgcgtt
      361 ttcgaaccgg tgctcgaact cggcgccgat cgctttgcga tcgatcttgc cgttcgggtt
      421 gcgcggcagc agtcccgtcc tcacgtcgac gatcgccgga accatgtacg ccggaagcat
      481 ctcgcggcaa tgcgcgacga ggtcatgggc ttcgagcgcg agtccgttgc gcggcgtcgc
      541 gacgaccacg atcgcctggc cgagcgaggg gtgcggcacg ccgaaggctg cggcctcacc
      601 cacccggccg gtcgcataga gcgcttcctc gatctcggtc gggctcacgc gatagcccga
      661 ggtcttgatc atctcgtcgc ggcgtccgac gaagtagagg aaaccttcgt cgtccatccg
      721 tacggtgtcg cccgagaaca cggcgatctc gggcaacacg agcccggcct cgcgtccggg
      781 caacggccgg tagcgttcgg cggtcttctc ggggtcgttc caatagccca agccgaccag
      841 tgcgccgcgg tggacgagct cgccgggctc gttcggttgg cagcgtgtac cgtcgggacg
      901 cagcacgaga atctccgcgt tcggaatcgc gttgccgatt gaatcgggac ggcgatcgac
      961 ctcctcagga ggaagataag tcgatcggaa cgcttcagtg aggccgtaca tcaggaacgg
     1021 cttcgcgcgc gggacgcgcg cgcgaagcgc cttcagcgtt tcgagcggca tccgaccacc
     1081 ggtattcgcg aagtagcgca ggt
//
LOCUS       DASUCJ010001370         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_901241, whole genome shotgun sequence.
ACCESSION   DASUCJ010001370 DASUCJ010000000
VERSION     DASUCJ010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901241"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFU90_15940"
     CDS             complement(<1..462)
                     /locus_tag="VFU90_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481338.1"
                     /translation="MFKRDPRDRFYRERLAGSTLASLAVHALLALLLVSLVFTSSEEG
                     ANENVEGGSLVTLERRTPAIANQPSAARIAIPVTHVPRIAPVRNASFAQPQTQRLPQN
                     RHELAVQAPTAPPNPREVPQQSPQPNPAPTENVYEVHPSTEVPAAPVNVPTV"
     gene            536..1969
                     /gene="pyk"
                     /locus_tag="VFU90_15945"
     CDS             536..1969
                     /gene="pyk"
                     /locus_tag="VFU90_15945"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5481339.1"
                     /translation="MEPIVQKRTKIVATVGPASREPAIMRSLFVSGANVIRLNFSHGT
                     PDEHGAVIRDVRALAAELGIQVALLQDLPGPKVRTGKLAGGVRSVRLERGAHFAVTVD
                     DVEGTGERASTSYRNLPADVAVGDRLYLQDGQITLRIRGKTATEIQTVVEFGGDLRPS
                     QGINYPDGTLNVESVTGRDLEYLEFGLEHGVDYVALSFVRSADDVVRAKDFIAKRGKR
                     VPVIAKIEKHEALDDLANIIRAADGIMVARGDLGIEIPIETVPIVQKRIIAECNRASK
                     PVVTATQMLESMIGSSRPTRAEATDVANAILDGTDAVMLSGETAIGLYPTEAVRMMAE
                     IAREVEKAYPHPALRDRRLEDVEPTVAASIAEAATRASEELNIPYIVTGTTTGNTAHH
                     IAAFRPRARIIALTPDPVVARRLALLWGTESLLIDSYNSIDVLLYMTEREMLHAGLVS
                     PGDSIAFTTGMPVGAGGTNVLKIHQIS"
     gene            complement(1964..>2760)
                     /locus_tag="VFU90_15950"
     CDS             complement(1964..>2760)
                     /locus_tag="VFU90_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481340.1"
                     /translation="AELDAVNARLDALERLERKYGGSVEAALAHAQSARAAVEAFEGR
                     DRREAGLKIEVEAARAELDAAAKSLAALRRKAASALVKRVAAELPELALGAGRFDVSF
                     EALERIGADGGDRVEFLFAANAGEPARALTRVASGGELSRLLLALIVVLANARDDAAA
                     LVFDEIDTGIGGATGTAVGARIGRLAREGQVVCVTHLAQLATWADRHYVLEKTERKDA
                     TTISVREISAAGERTEELARMLSGEKHDVALKHARALLQRARATPL"
BASE COUNT          450 a          979 c          889 g          442 t
ORIGIN      
        1 gaccgtcggt acgttcaccg gtgcggccgg cacctcggtg ctcggatgaa cttcgtagac
       61 gttttcggtc ggcgccggat tgggctgcgg gctctgctgc ggcacttccc gcggattcgg
      121 cggagcggtc ggcgcctgca ccgccagctc gtggcggttc tgcgggagcc gctgcgtctg
      181 cggctgtgcg aacgacgcgt tgcgaaccgg cgcgatgcgc gggacgtgcg tgaccgggat
      241 tgcgatgcgc gcggcggacg gctggttggc gatcgccggc gtgcggcgtt ccagcgtcac
      301 cagcgatccg ccctcgacgt tctcgttcgc gccctcctcc gacgaggtaa acacgagact
      361 caccagcagc agcgcgagca gcgcgtgtac ggccagcgac gccagcgtac ttcccgcaag
      421 ccgctcccga taaaatcggt cccggggatc gcgtttgaac atagcagccc gcgttaagtg
      481 cgcttaacgc atcgcaggag cctgcctgag ctttcgcgca agccaaaagc cttccatgga
      541 accgatcgta caaaaacgga cgaagatcgt tgcgaccgtc ggcccggcgt cgcgcgagcc
      601 cgcaatcatg cgttcgctgt tcgtcagcgg cgcgaacgtc attcgtttga atttttcgca
      661 cggcacgccc gacgaacacg gcgccgtgat tcgcgacgta cgcgcgctcg ccgccgagct
      721 cggcattcag gtcgcgctgc tgcaggatct tcccggcccg aaggtgcgta ccggaaaact
      781 cgccggcggc gtgcggtcgg tgcgcttgga acgcggcgcg catttcgccg tcaccgtcga
      841 cgacgtcgag gggaccggcg agcgcgcgag cacgtcgtat cgcaacttgc ccgccgacgt
      901 cgcggtcggc gaccgcctat atttgcagga cggccaaatc acgctgcgca tccggggaaa
      961 aaccgcgacc gaaattcaga ccgtggtcga gttcggcggc gacttacggc cgtcgcaggg
     1021 catcaactac cccgacggta cgctcaacgt cgaatccgtc accggacgcg acctggagta
     1081 tttggagttc gggctcgagc acggcgtcga ctacgtggcg ctgtcgttcg tgcgcagcgc
     1141 cgacgacgtc gtccgcgcca aagacttcat cgccaagcgc ggtaagcgcg ttccggtcat
     1201 cgcgaagatc gaaaagcacg aggcgctcga cgatctcgcc aacatcattc gcgccgcgga
     1261 cggcatcatg gtggcgcgcg gcgatctcgg gatcgagatt ccgatcgaaa ccgtcccgat
     1321 cgttcagaaa cgaatcatcg ccgaatgcaa ccgcgcgagc aaaccggtcg tgaccgccac
     1381 ccaaatgctg gaatcgatga tcggatcgtc gcgccccacg cgcgcggaag ccaccgacgt
     1441 cgccaacgcg atcctcgacg gcaccgatgc ggtcatgctc tcgggcgaaa ccgcgatcgg
     1501 cctttatccc accgaagcgg tgcgcatgat ggccgagatc gcccgcgagg tggaaaaggc
     1561 gtacccgcac ccggcgctgc gcgaccggcg gctggaagac gtcgagccga ccgtggccgc
     1621 ctcgatcgcc gaagccgcca cgcgcgccag cgaggagctg aacattccct acatcgtcac
     1681 cgggacgacg accggcaaca ccgcccacca catcgccgcc ttccgtccgc gggcgcgaat
     1741 catcgcgctg acgccggatc cggtggtcgc gcgccggctc gcgctcttat ggggaaccga
     1801 atcgctgctg atcgattcgt ataactcgat cgacgtcttg ctctacatga ccgagcgcga
     1861 gatgctgcac gccggcctgg tgagtcccgg cgactcgatc gccttcacca ccgggatgcc
     1921 ggtcggcgcc ggcggcacca acgttctcaa aatccaccag atttcataac ggcgtcgctc
     1981 gcgccctctg cagcagcgcg cgcgcgtgtt tcagcgcgac gtcgtgcttt tcgcccgaga
     2041 gcatccgcgc cagctcctcg gtgcgctcgc cggcggcgga gatctcccgc accgaaatgg
     2101 tcgtcgcgtc tttgcgctcg gttttttcca gcacgtagtg gcgatccgcc caggtcgcca
     2161 gctgcgccaa gtgcgtgacg caaacgactt gcccctcgcg cgccagccgt ccgatacgcg
     2221 ctccgacggc cgttccggtc gcgccgccga tcccggtgtc gatttcgtcg aagaccagcg
     2281 ccgcggcgtc gtcgcgagcg ttggccaaca cgacgatcag cgccagcagg aggcgcgaca
     2341 gctcgccgcc cgacgccacc cgcgtcagcg cgcgggccgg ctcgccggcg ttggcggcaa
     2401 acagaaactc gacgcgatcg ccgccgtcgg cgccgatgcg ttccagcgcc tcgaaggaaa
     2461 cgtcgaaacg tccggccccg agcgccagct ccggcagttc cgccgccacg cgtttgacga
     2521 gcgccgacgc cgccttgcgg cgcagcgccg ccagcgactt cgccgccgcg tcgagttcgg
     2581 cacgcgccgc ttcgacttcg attttcaggc cggcttcgcg gcggtcgcgt ccttcgaacg
     2641 cctcgaccgc cgcccgcgcg ctttgcgcat gcgcgagcgc cgcctcgacg ctgcccccgt
     2701 atttgcgttc gagccgttcg agcgcgtcga ggcgcgcgtt gacggcgtcg agctccgccg
//
LOCUS       DASUCJ010001371         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1405335, whole genome shotgun sequence.
ACCESSION   DASUCJ010001371 DASUCJ010000000
VERSION     DASUCJ010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405335"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFU90_15955"
     CDS             <1..358
                     /locus_tag="VFU90_15955"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5481341.1"
                     /translation="TRVGVDVALATGAMTLPQAIAYFQKNAGIDYATARGEATRFAMD
                     PGQAIDYLIGKTQIETLLGMARDAEGTAFTLEGFHDRLLSYGTVPFSTIRYEWLGDDG
                     WLAQGVLEPLAPQQFP"
     gene            355..912
                     /gene="rsmD"
                     /locus_tag="VFU90_15960"
     CDS             355..912
                     /gene="rsmD"
                     /locus_tag="VFU90_15960"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5481342.1"
                     /translation="MSPRISSGSLGSRKLRSPKGMHVRPTPGRVKESLFSILAERLEG
                     ARVLDLFAGTGAIGFEAASRGAARVVAVESHRETAHAIEEAARELGVDKTVSVFAAPA
                     ERALYRLDGPFDIVYLDPPFAHEVPLQLLRLLRERNLVAPGGLVIYEHAARTILPEIP
                     GYRSTREEVYGDVALAFLAPEPSGA"
     gene            909..1430
                     /gene="coaD"
                     /locus_tag="VFU90_15965"
     CDS             909..1430
                     /gene="coaD"
                     /locus_tag="VFU90_15965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5481343.1"
                     /translation="MIDGKPSRSTRAIYPGSFDPPTYGHLDVVQRAARCFGELVVAIV
                     VNPQKRSPMFSLDERVAMLQESIAGIPNTRVEHFRGLLADYVKASGADVIVKGLRVVS
                     DFESEMSAALMNRSLSDVDTFFLMSDQKYSFVSSSLVKEVFFLGGDVGSLVPEPVLRV
                     MTAKRAALQDART"
     gene            1444..2010
                     /locus_tag="VFU90_15970"
     CDS             1444..2010
                     /locus_tag="VFU90_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481344.1"
                     /translation="MSVYRVLDKLEAYVHEGTWLPAGYRVLSEERLLELVEKIRASLP
                     EEVGRAKIIAKGQDRVMRAAAEKAQAIVDEAATKHEELLDQNEIVKRARTTADVVLRE
                     AEERARKIREGADQYAAQVLSEMEARLGGALGAVRKGRESLARRPEAGQPAPAGQPLA
                     EAAAKSKRAAFDSQTAGEETAALESVEV"
     gene            2034..>2404
                     /locus_tag="VFU90_15975"
     CDS             2034..>2404
                     /locus_tag="VFU90_15975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5481345.1"
                     /translation="MRRFASMLAFVSLTAVPALAAQFGGGISGYVTDVKTGQPIAHAT
                     ILYYRAPYVENVTVPKQLVTDRHGFFSDVTLEPGRYVLMARFPDKVEGCAVDDVMDGE
                     STRVKIQMGYDTILCSGPRVHP"
BASE COUNT          427 a          751 c          807 g          419 t
ORIGIN      
        1 cacccgcgtc ggggtcgacg tggcgctcgc gaccggcgcg atgacgttgc cgcaagcgat
       61 cgcgtacttt cagaaaaacg ccggtatcga ctacgcgacg gcccgcggcg aagcgacccg
      121 ctttgcgatg gatccgggcc aggccatcga ctatctcatc ggcaagacgc agatcgagac
      181 gctgctgggg atggcacgcg acgccgaggg gaccgccttc acgctggaag gcttccacga
      241 tcggctgctc tcctatggga ccgtgccgtt ctcgacgatt cgctacgagt ggctgggcga
      301 cgacgggtgg ctcgcgcagg gggttctcga gccgctcgcg ccgcaacagt tcccatgagc
      361 cctcgcatct ccagcggttc gctcgggagc cgtaagcttc gttcgcccaa agggatgcac
      421 gtacgtccga cgccgggtcg cgtcaaagag tcgctctttt cgattctggc cgaacgcctc
      481 gagggagctc gcgtcctgga tttattcgcc ggcacgggag cgatcgggtt cgaagcggcc
      541 tcgcgcggcg cggcgcgggt cgtcgcggtc gagtcgcacc gtgaaaccgc gcacgcgatc
      601 gaggaggcgg cacgcgaact cggcgtcgac aagaccgtca gcgtctttgc cgcgccggcc
      661 gaacgagccc tgtatcggct ggacggtccg ttcgacatcg tctatctgga tccgccgttc
      721 gcgcacgagg tcccgctgca gttgctgcgg ctgctgcgcg agcgcaatct cgtcgcgccg
      781 ggcggcctcg tgatctacga gcacgcggcg cgaacgattc ttcccgagat accggggtat
      841 cgctcgacgc gcgaagaagt ttacggcgat gtcgcactcg cgttcctcgc accggaaccg
      901 tcgggcgctt gatcgacggt aagccgtcgc gcagcacgcg cgccatctat cccgggtcgt
      961 tcgatccacc gacgtacggc cacctcgacg tcgtccaacg cgccgcacgg tgcttcggcg
     1021 aactcgtcgt cgcgatcgtc gtgaacccgc agaagcgcag cccgatgttt tcgctcgacg
     1081 aacgggtcgc gatgctccag gaatcgatcg ccggaattcc aaacacccgg gtcgagcatt
     1141 ttcgcgggtt gctcgccgac tacgtcaaag cctccggcgc cgacgtcatc gtcaaagggt
     1201 tgcgcgtggt gtcggacttc gaaagcgaaa tgtcggccgc tttgatgaac cgttccctgt
     1261 cggacgtgga tacgttcttc ctcatgtcgg atcagaagta ctcattcgtt agctcgagtc
     1321 tggtgaagga agtatttttt ctagggggcg acgtcggctc gttggtcccc gaaccggtgt
     1381 tgcgggtcat gacggccaag cgcgccgcgt tacaagacgc tcgtacgtaa aacggaggta
     1441 gcaatgtcgg tttatcgcgt gctggacaaa ctcgaagcgt acgttcacga aggtacttgg
     1501 ctgcccgcag ggtaccgcgt tctttcggaa gagcggctgc tggagctggt cgagaaaatc
     1561 cgggcttcgc ttcccgaaga ggtcggccgg gcgaagatca tcgccaaagg tcaagaccgc
     1621 gtgatgcgcg ccgccgcgga gaaggcgcaa gcgatcgtcg acgaggcggc caccaagcac
     1681 gaggagctgc tcgaccaaaa cgagatcgtc aagcgcgcgc gcacgacggc cgacgtcgtc
     1741 ctgcgagaag cggaggagcg ggcgcgcaag attcgcgagg gcgccgacca gtatgcggca
     1801 caggtgctga gcgagatgga agccaggctc gggggcgcct tgggggcggt ccgcaagggc
     1861 cgggaatcgc ttgctcgccg cccggaggcg ggtcagccgg cgccggccgg ccagcccttg
     1921 gccgaagccg cagccaagag caagcgggcg gctttcgatt cgcagaccgc cggcgaggaa
     1981 acggccgctt tggaatcggt cgaagtttga ccgaaacggg gtataagcgc tagatgagac
     2041 gttttgcaag catgctcgcg ttcgtgagct tgaccgccgt cccggcgctg gcggcgcagt
     2101 tcggaggggg tatctccggc tacgtcaccg acgtgaagac cggtcagccg atcgcacacg
     2161 caacgatcct gtactaccgc gctccctacg ttgagaacgt gaccgttccg aagcagctgg
     2221 tcaccgaccg ccacggtttc ttcagcgacg ttaccctcga gccgggccgc tacgtgctga
     2281 tggcgcgctt cccggacaag gtcgagggct gcgccgtcga cgacgtgatg gacggcgaga
     2341 gcacgcgtgt caagattcaa atgggatacg acacgatctt gtgttcgggc ccgcgcgtcc
     2401 atcc
//
LOCUS       DASUCJ010001372         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1176267, whole genome shotgun sequence.
ACCESSION   DASUCJ010001372 DASUCJ010000000
VERSION     DASUCJ010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176267"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFU90_15980"
     CDS             complement(<1..932)
                     /locus_tag="VFU90_15980"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU5481346.1"
                     /translation="MSTQIKHPVHPGEVPSTPSEASRWQWLTTALPWASLVARIVGAG
                     VFGYAGISKIGDPAGGVRAVRAYRILPESLVHPVAYGLPAFEIVLAVLLFLGVASRVV
                     GAITAGLLAVFIAAVSSAGIRGLRIDCGCFGGGGQVEQTHYLLEIGRDSLLLLLLLAV
                     VFAKRSRLSIDERLAADPGEKPLPPGMSGSRRRKLEEVRKEQARRRQQLRGWVAISVA
                     VVALAGAAIGGIAANAAPSSTKTAAVVVPPGATGSGGIVVGSAAAPIKLIAYEDPQCP
                     VCKDFEQVNGPTLHAAINAGKVSVEYRVRSFIGV"
     gene            complement(978..1805)
                     /locus_tag="VFU90_15985"
     CDS             complement(978..1805)
                     /locus_tag="VFU90_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481347.1"
                     /translation="MVGSLLPGRMARQPLERSRLRPGLLPPLTPEYSLNVRTLGLFGA
                     FEWRRYWSRRLADVSDAGAIPARVMRAVVFASVAIVLLLLLRAKSDGANPPLAVVLSS
                     FALLAGLAWAFGSRERGAIAMLTGFIATQLALHIGYLFVTTGQLAHPGGAGLFCSPAS
                     VTAGACAPTNRGGMLLLVVQLTVALALALVMRGADAECWQLVRRPTLVISKAVRRLLT
                     AVAAIALTPPVERVISVVAEWVSPRPPRRLLLSHECGRRGPPACLVTSSLVAVPSFS"
BASE COUNT          380 a          645 c          593 g          247 t
ORIGIN      
        1 tcaacgccga tgaacgaccg gacgcggtac tcgaccgaaa ccttgccggc gttgatcgcc
       61 gcatgcaacg tcggaccgtt gacctgctcg aagtccttgc agacagggca ttgcgggtct
      121 tcatacgcga tcagcttgat cggggccgcg gcgcttccca caacgatgcc acccgagccg
      181 gtcgctccgg gcggaacgac gaccgccgcg gtctttgtgg acgacggcgc ggcattcgcc
      241 gcaatcccgc cgatggcggc gccggccagc gcgacaaccg cgacgctgat cgcgacccag
      301 ccgcgcaact gttgacgacg gcgagcttgc tctttgcgga cctcttcgag cttgcgccgc
      361 cgcgagccag acatgcctgg aggcaacggt ttttcgccgg ggtcggcagc caatcgctcg
      421 tctatcgaga gtcgacttcg cttcgcgaac accaccgcga gcagcaacag cagcagcagc
      481 gagtcgcggc cgatttcgag caggtaatgg gtttgttcga cctggccgcc gcccccgaag
      541 cagccgcagt cgatccgtag cccgcgtatc ccggccgacg agaccgcggc gatgaagacg
      601 gcgagcagtc ctgcggtgat ggctccgacc actcggctgg ccacgccgag aaacagcagc
      661 acggctagca cgatttcaaa agccggcaga ccgtaggcga ccggatgcac gagagactcc
      721 ggaaggatgc ggtacgcgcg caccgcgcgc acgccgcctg ccggatcgcc gatcttcgag
      781 atgcccgcgt agccgaacac gccggcgccg acgatgcgcg cgaccagcga cgcccacggc
      841 agcgccgtgg taagccactg ccagcgggag gcttcgcttg gtgttgacgg aacttcaccc
      901 gggtgcaccg gatgcttgat ctgggttgac atggcactcc tgaaaagtcg agacgagcgg
      961 cgaccgctcg tctcgcatca ggaaaacgag ggcacggcca ccagggacga agtcaccaga
     1021 cacgccgggg gcccgcgtcg tccgcactcg tgcgacaaca gcaggcgacg tggcggccgc
     1081 ggcgaaaccc actcggccac aacggaaatc acccgctcaa cgggcggcgt caacgcaatc
     1141 gcggcaaccg cggtgagcaa ccggcgaacc gctttcgaga tcaccaacgt cggacgacga
     1201 accagttgcc aacactcggc gtcggcgcct cgcatgacaa gcgcaagcgc gagcgccacg
     1261 gtcagctgca ccaccagcaa cagcatgccg cctctgttgg ttggcgcgca tgcgccagca
     1321 gtgacgctgg caggactgca aaaaagaccg gcgccaccgg gatgcgcgag ttggccagtc
     1381 gtcacgaaca ggtagccgat gtggagcgcc aactgggtcg cgatgaaccc ggtcagcatc
     1441 gcgatcgcgc ctcgctcgcg cgatccgaac gcccacgcca atccggccaa gagcgcgaat
     1501 gaagagagca caaccgcgag cggcgggttc gcgccgtccg actttgcccg caacaacagc
     1561 aacaacacga tcgcgacgga tgcgaaaacg acggcgcgca tcacccgcgc cgggatcgca
     1621 cccgcgtcgc tcacgtcagc caacctcctc gaccagtatc ggcgccattc gaacgcgccg
     1681 aacaggccta aggtcctgac atttagcgaa tattcaggtg tcagcggcgg tagtagcccc
     1741 ggccgaagcc gcgatcgttc cagtggttgt cgcgccattc ggcccggtag gagcgacccg
     1801 accacccgcg tcggccgccg ccggcgaaca tccgagctgc gaagaagagg agcacgatga
     1861 acccg
//
LOCUS       DASUCJ010001373         1473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_366276, whole genome shotgun sequence.
ACCESSION   DASUCJ010001373 DASUCJ010000000
VERSION     DASUCJ010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366276"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFU90_15990"
     CDS             <1..745
                     /locus_tag="VFU90_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5481348.1"
                     /translation="PSLTPAPRPTPAPAAQGRETRERMTTRRKRIAENLVQAQHATAH
                     LTTFNEVDMSAVMGVRERLKERVEKQHGVRLTFMPFFARAATMALNAYPVVNAQIDGD
                     NIIYKHYVNLGIAVASDQGLVVPVIKDADRMGMIEFSRQLSAVAARAREGKLTMDDLT
                     GGTFTITNGGVFGSLVSTPILNYPQAGILGLHKIQDRPVALDRQVVIRPMMYIALSYD
                     HRLVDGQQAVLFLVRMKELMEDPAAMLVD"
     gene            806..>1473
                     /locus_tag="VFU90_15995"
     CDS             806..>1473
                     /locus_tag="VFU90_15995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5481349.1"
                     /translation="MAETLSPDVVIIGGGPGGYVAAIRAAQLGLSTVCVEMDATLGGT
                     CVNVGCIPSKALLQSSEHFEFTRTHLSDHGVKVGDVTLDLATMMTRKDRVVSANTRGI
                     EYLFRKNNVTWAKGRGTLRPGNVVEVRAADGSTTTYRARDVVIATGSVPIALPFLPFD
                     EDRVLSNVGGLAMRDVPGHLVVIGGGVIGLELGSVWRRLGAKVTVVELLPTILAGNDA
                     EVVK"
BASE COUNT          252 a          493 c          478 g          250 t
ORIGIN      
        1 gccgtcgctt actcccgcgc ctcgcccaac gccggcgcca gctgcgcaag gccgcgagac
       61 gcgagagcgg atgaccacgc gtcgcaaacg catcgccgag aatctcgtgc aggcacagca
      121 cgcaaccgcg cacctcacga cgttcaacga agtcgacatg tcggcagtga tgggcgtgcg
      181 cgagcggctc aaggagcgcg tcgagaaaca gcacggcgtc agactcacgt tcatgccctt
      241 cttcgcgcgt gcggcgacga tggcgttgaa cgcctacccg gtcgtgaacg cgcagatcga
      301 cggcgacaac atcatctaca agcactacgt gaacctcggc atcgccgtcg cgtccgacca
      361 gggcctcgtc gttcccgtca tcaaagacgc cgatcgcatg gggatgatcg agttcagccg
      421 ccagctctct gcggtggccg cgcgcgcgcg cgagggcaag ctcacgatgg atgacctcac
      481 cggcggcacg ttcacgatca ccaacggtgg agtcttcggt tcgctcgtct cgacgccgat
      541 cctgaattat ccgcaggccg gcatcctcgg gctgcacaag atccaggacc gtccggtggc
      601 gctcgacagg caggtcgtga tccgcccgat gatgtacatc gccctgtcgt acgatcatcg
      661 cctcgtcgac ggccaacagg ccgtactctt tctcgtgcgc atgaaggagt tgatggagga
      721 tccggcggcc atgctcgtcg attgatcgcg cacgcgggcg cgcggccccc atcgcccacg
      781 cgcctccctg ttcactggtt aggcaatggc tgaaacgctc tctcccgacg tcgtcatcat
      841 cggcggcggc cccggcggtt acgtggccgc gatccgcgcg gcgcagctcg gtctctccac
      901 ggtgtgcgtc gagatggacg ccacactcgg cggcacgtgc gtgaacgtcg ggtgcattcc
      961 atcgaaagcg ctgctgcagt cgtccgagca cttcgagttc acgcgcacgc atctctccga
     1021 ccacggcgtg aaggtcggcg atgtgacgct ggacctcgcc accatgatga cgcgaaagga
     1081 tcgcgtggtg tcggcgaaca cgcgcggcat cgagtacctg ttccggaaga acaacgtcac
     1141 gtgggcgaag ggtcgcggga cgctgcggcc cggcaatgtg gtcgaggtcc gcgccgcgga
     1201 cggatcgacc acgacgtatc gcgcccgcga tgtcgtgatc gcgaccggca gcgtccccat
     1261 cgcgctgccg tttcttccgt tcgacgaaga tcgcgtgttg tccaacgtcg gcggcctggc
     1321 gatgcgcgac gtccccgggc atctggtcgt gatcggcggc ggcgtcatcg gactcgagct
     1381 cggctcggtc tggcgccggt taggcgcgaa ggtcaccgtg gtcgagcttt tgccgacgat
     1441 tctcgccggc aacgacgccg aagtggtgaa gga
//
LOCUS       DASUCJ010001374         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1115242, whole genome shotgun sequence.
ACCESSION   DASUCJ010001374 DASUCJ010000000
VERSION     DASUCJ010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115242"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFU90_16000"
     CDS             complement(<1..746)
                     /locus_tag="VFU90_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481350.1"
                     /translation="MFRSIFARAAIALLPLTLGITVTSPGTVTTVVDPRLFSAPSGDE
                     HLHGIVRDPISGDIYVGDWNKLTLSNTPFFGPFIENKDSIRRINALKEVSVVTFVVSP
                     NAIAYSPSDRQIYVVVGSVECNNRSGPRSTGPTLNGIVALDPATGKTRVLAGGNAGVS
                     NGPGAQALFSEPAGIASDPGSGAFFVSEGCQNRIREVDPSGTAATLAGSGAKGTADGT
                     GLEASFNDPRGIAYCENEHTLYIADSGNNE"
     gene            complement(846..1796)
                     /locus_tag="VFU90_16005"
     CDS             complement(846..1796)
                     /locus_tag="VFU90_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5481351.1"
                     /translation="MERVALFGAAGAIGHALAPELQRRQIAYRTAGRTVVRLQRDFPR
                     SEAVRADFLSGEGVAAAASGVDTVFYLAGAPYTEFHKHPIMVRNALDAAQAAGVRRFV
                     HVAPVYSYGPALTRPVPESQPHVPNTRKGRFRLEQERAVLERNGGGFATMVVHLPDFY
                     GPEAELSYANAFMREALAGKTASFVGPLDAQREFVYVPDAAAPLLQLAELDDAYGRCW
                     NLGGRAVEARAFADEVFAVLGKRPKYRSVPKFVVRAAGVAVPFMREVAEMYYLFDSDF
                     VLDDGALRQRLGGYEKTPLAQGIRQTLEWMREHPDDGSRT"
     gene            complement(1896..3410)
                     /locus_tag="VFU90_16010"
     CDS             complement(1896..3410)
                     /locus_tag="VFU90_16010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5481352.1"
                     /translation="MIQGLGPRDLIGLLSDVGATLSSESQPAEILQALVSACVRDACS
                     FAAAYLDAGEPPAAVAAREGIATEKLSGLPLAALDASAHGFAALRREPLRMKGRPIGT
                     LVLGLTDQLEPAALAAVQTAAMLAAASVDIAQSNANQRRIADRLQRAMLPLTLPSRDS
                     VTFFSAYRPATDETLVGGDWYDVFELGDGSLGFSIGDVTGHGLDAAIVMIEARLAIRS
                     AAAAMESPAAVLEFVNHLLSLDSPAGSATALVGTYDPQTCTLRYACAGHPPPFYVGSD
                     RRVIALTGGGLPLGINDTVGAADWRVTLDPGASLALFTDGLLEYGRDILAGERRLVDA
                     LGRERVMAAENPAAELFETVLSGTRNRDDVAVLVIRCDRPPAEKLELRYSAAPRFAAI
                     ARSAISSYMRSLRFDAATIGEVLSASGEAIANAIEHGSYNESSPGFEVCMERAPGGLQ
                     LTVESSGHWKRSGGRDDRGFGIRIMRAIASDLSFASTSRTTVARLAFDCEKSIG"
     gene            3542..>5451
                     /locus_tag="VFU90_16015"
     CDS             3542..>5451
                     /locus_tag="VFU90_16015"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5481353.1"
                     /translation="MSTVSYSNDPLETQVVKDRLDPALLLGALRAFRNGDFSCRLPND
                     LVGIAGEIADAFNDCIDLNDRFSRELKRIRDAVGRDGLVSKRAQLAGGRASWNGYVES
                     VNALIGDLVFPMAAAGRVLAAVAKGDLSQRMPAESEGRPLKGEFLRSSRTINTMVDRL
                     SAFASEVTRVAREVGSEGKLGGQAVVEGVAGTWKDLTDSVNTMAGNLTAQVRNIAEVT
                     TAVANGDLSRKITVEARGEIVSLKSTVNTMVDQLNAFASEVTRVAREVGSEGILGGQA
                     QVSGVSGTWKDLTDSVNFMALNLTSQVRNSADVTTAVANGDLSKKITVDVRGEIAELK
                     STINTMVDQLNAFASEVTRVAREVGSEGKLGGQADVKGVSGTWKDLTDSVNVMAGNLT
                     SQVRNIADVTTAVAKGDLSKKITVDVRGEIAELKSTINTMVDQLNGFASEVTRVAREV
                     GSEGRLGGQADVRGVSGTWKDLTDSVNFMASNLTSQVRNIAEVTTAVAKGDLSKKITV
                     DVRGEIAELKRTVNTMVDQLNALASEVTRVAREVGSEGKLGGQAQVQGAAGTWKDLTD
                     SVNMMAGNLTSQVRNIADVTTAVANGDLSKKITVDVRGEILELKNTINTMVDQLNTFA
                     NEVTRVAREVGT"
BASE COUNT         1002 a         1823 c         1738 g          888 t
ORIGIN      
        1 atttcgttgt tgccgctgtc ggcaatgtag agcgtgtgct cgttttcgca gtaagcgatt
       61 ccgcgcggat cgttaaagct cgcctccaac ccagtcccat cggccgttcc tttcgcgccg
      121 gatccggcca gcgtcgcggc ggttccgctc gggtcgacct cgcgaatccg gttttgacag
      181 ccttccgata cgaagaacgc tcccgaaccc gggtccgacg cgatgccggc cggctcggag
      241 aagagagcct gagcccccgg cccgttcgag acgcccgcat ttccgcccgc caagacgcgc
      301 gtttttccgg tggcggggtc gagcgcgacg attccgttga gcgtcgggcc ggtcgagcgc
      361 ggaccgctcc ggttgttgca ctcgacgctt ccgacgacca cgtagatttg gcggtcggaa
      421 gggctatacg cgattgcgtt cggcgacacg acgaaagtga cgaccgacac ttccttgagc
      481 gcgttgatgc gacgaatcga atccttgttt tcgatgaacg ggccgaaaaa cggcgtgttg
      541 ctgagggtaa gcttgttcca atcgccgacg tagatatcgc cgctgatcgg atcgcggacg
      601 atgccgtgaa ggtgctcgtc gccgctgggc gcgctaaaaa gccgcggatc gacgaccgtc
      661 gtaacggtgc cgggcgacgt tacggtaatg ccgagcgtga gcgggagaag cgcgatcgcg
      721 gcgcgcgcga agatcgaacg gaacatggac tcttcctccg gtaaacccga aacctgggcc
      781 ttgtgcccgt cccagtctag cgggagggta agaagaaccg gtgagcccct cttcgcaggt
      841 gcttctcagg tccggctgcc gtcgtcgggg tgttcccgca tccactcgag cgtttgacga
      901 atgccttgcg cgagcggggt tttctcgtat ccgcccaacc gctgccgcag cgcgccgtcg
      961 tccagcacga aatcgctgtc gaacaggtag tacatctccg cgacctcgcg catgaacgga
     1021 accgccacgc cggccgcccg cacgacgaat tttggaaccg accggtattt cgggcgcttt
     1081 ccgaggacgg caaacacctc gtcggcgaac gcgcgcgctt cgaccgcccg gcctccgagg
     1141 ttccagcagc ggccgtacgc atcgtcgagc tccgcgagct gcagcagcgg cgcggcggcg
     1201 tcgggcacgt agacgaactc gcgctgcgcg tccagcggac cgacgaacga tgccgtcttg
     1261 ccggccagcg cttcgcgcat gaaggcgttg gcgtagctga gctcggcttc cggtccgtaa
     1321 aaatccggta ggtgcactac catcgtcgcg aagccgccgc cgttgcgctc gaggaccgcc
     1381 cgttcctgtt cgaggcgaaa gcggcccttc cgcgtgttgg ggacgtgcgg ctggctttcg
     1441 gggaccggcc gcgttagcgc ggggccgtaa ctgtagaccg gcgcgacgtg cacgaaacgc
     1501 cgtactcctg cggcttgcgc ggcgtcgagc gcgttgcgca ccatgatcgg atgtttgtgg
     1561 aactcggtgt acggcgcacc cgcgagatag aagaccgtat ccacgccgct cgcggcggcg
     1621 gccactcctt cgccgctcag aaaatcggcg cgcaccgctt cggagcgcgg aaaatcgcgc
     1681 tgcaggcgga cgaccgtacg gcccgccgta cgataggcga tttgccgccg ttgcagttcc
     1741 ggggcgagtg cgtgcccgat cgcaccggcc gcgccgaaca gcgctacgcg ttccataata
     1801 aaggcgcccg ccgaaataac ggccggctag gcgcctcggg gtcggacgtt ggatagcgta
     1861 cgatcatttt ctcccggctc gcggcggatc aaacgtcacc caatggattt ctcgcagtcg
     1921 aatgccaaac gagcgaccgt cgtccggctc gtcgacgcga agctcaagtc gctggctatc
     1981 gcacgcatga tgcgaatgcc gaacccgcga tcgtcccttc cgccggaccg cttccaatga
     2041 ccgctgcttt cgaccgtcag ctgcaaaccg ccgggggctc gttccatgca gacttcgaag
     2101 ccgggcgacg attcgttgta cgaaccgtgc tcgatcgcat tggcgatcgc ttcgcccgac
     2161 gccgagagga cctcgccgat cgttgccgcg tcgaaccgca gcgaccgcat gtagctcgaa
     2221 atcgccgacc gggcgatcgc ggcgaaccgc ggcgcggccg aataccgcag ctcgagtttt
     2281 tccgccggcg gccggtcgca gcgaatgacg agcaccgcga cgtcgtcgcg gtttcgggtg
     2341 ccgctcagaa cggtttcgaa cagctcggct gccggatttt cagccgccat cacgcgctcg
     2401 cggccgagcg cgtcgaccaa acgccgctcc cccgcgagaa tgtcgcgccc gtactccaag
     2461 aggccgtcgg tgaagagcgc cagcgatgcg ccgggatcga gcgtcacccg ccagtcggca
     2521 gctcctaccg tgtcgttgat gccaagcggc agccctccgc ccgtcagagc gatcacgcgg
     2581 cggtcgctcc cgacgtaaaa cggaggcgga tgcccggcgc acgcgtagcg cagcgtgcac
     2641 gtctgcggat cgtacgtccc gacgagggcg gtggccgaac cggccggcga gtcgagcgac
     2701 agcaggtgat tgacgaactc cagcaccgcc gccggcgatt ccatcgccgc ggccgccgag
     2761 cggatcgcga gccgcgcctc gatcatcacg atcgccgcat ccaaaccatg gcccgtcacg
     2821 tcgccgatcg agaagccgag ggagccgtcc cccagttcga agacgtcgta ccaatcgccg
     2881 cccacgagcg tctcgtcggt tgccggccga tacgcactga agaacgtcac gctgtcgcgg
     2941 ctgggaagcg tcagaggaag catggcgcgc tgcaggcggt ccgcgatccg gcgctgattg
     3001 gcgttggatt gcgcgatgtc gaccgaggcg gcagccagca tcgcggccgt ctgcacggcg
     3061 gcgagcgcgg ccggctcgag ctggtcggtg agccccaaaa cgagcgttcc gatcggacgg
     3121 cctttcattc gtaacggctc gcggcggaga gcggcaaatc cgtgggcgga ggcatccaac
     3181 gcggccagcg gcagcccgga cagcttctcc gtcgcgatcc cctcccgggc ggcgacggcc
     3241 gccggcggct cgccggcgtc taggtaggcg gccgcaaacg agcaggcgtc gcggacgcac
     3301 gccgatacca aagcctgcag aatctctgcc ggctggctct cggaggataa ggtcgcgccg
     3361 acgtccgaca acaacccaat caggtctcgc ggcccgaggc cttggatcat gcggaaagta
     3421 tccccacgga cgccccccga ccaaaccagc gccctgtttc tttttcgccg ccctgggtag
     3481 aaccgaggct gagcgcgctg ttggtttggc gctctggcga ttcggctggg aggcttccga
     3541 gatgtcgacg gttagctatt ctaacgatcc tctcgaaacg caggtcgtaa aggaccggct
     3601 cgaccccgcg ttgctgctgg gcgcgctgcg cgcctttcgg aacggcgatt tctcgtgccg
     3661 ccttcccaac gacttagtcg ggatcgccgg cgaaattgcc gacgccttca acgactgtat
     3721 cgacctcaac gatcgttttt cgcgcgagct caaacgcatc cgcgacgccg tcggccgcga
     3781 cggactcgtg tcgaagcgcg cgcagctggc gggcggccgc gcttcgtgga acggttacgt
     3841 cgaatccgtc aacgcgttga tcggcgacct cgtctttccg atggctgccg cgggccgcgt
     3901 gctcgccgcc gtcgcgaaag gcgatctttc gcagcgcatg ccggccgaat ccgaagggcg
     3961 gccgttaaaa ggcgaattct tgcgatcctc ccgcacgatc aacacgatgg tcgatcgctt
     4021 gagcgcgttt gcctccgagg tgacgcgcgt ggcgcgtgaa gtcggctccg aaggcaagct
     4081 cggcggacag gccgtggtcg aaggcgtcgc cggcacctgg aaggatctca ccgattcggt
     4141 caacacgatg gccggcaatc tcaccgcgca agtgcgcaat atcgccgagg tgacgaccgc
     4201 cgtcgccaac ggcgaccttt cgcgcaagat tacggtcgaa gcccgcggcg aaatcgtctc
     4261 gctcaagagc accgtcaaca cgatggtcga tcagctcaac gcattcgcgt cggaggtcac
     4321 gcgcgtcgcc cgcgaagtcg gttcggaagg catcctgggc ggccaggccc aggtgagcgg
     4381 cgtcagcggc acctggaaag acctcaccga ctcggtcaac ttcatggcgc tcaatctcac
     4441 ctcccaggtg cgcaacagcg ccgacgtcac gaccgcggtc gccaacggcg atctttcgaa
     4501 gaaaatcacg gtcgacgtgc gcggcgaaat cgccgaactg aaatcgacca tcaacacgat
     4561 ggtcgatcag ctcaacgcct tcgcttccga ggtcacgcgc gtcgcgcgcg aagtcggttc
     4621 cgagggcaag ctcggcggac aggccgacgt caaaggcgtc agcgggacct ggaaagacct
     4681 caccgattcg gtcaacgtca tggccggcaa cctcacctcg caagtgcgca acatcgccga
     4741 cgtcacgacg gcggtcgcca agggcgatct ttcgaagaaa atcaccgtcg acgtgcgcgg
     4801 cgaaatcgcc gaactgaagt cgaccatcaa cacgatggtc gatcagctca acggttttgc
     4861 ctcggaggtc acccgcgtcg cgcgcgaagt cggctccgaa ggacggctcg gcgggcaagc
     4921 cgacgtccgc ggcgtcagcg gcacctggaa ggatctcacc gactcggtca acttcatggc
     4981 gtcgaacctc acctcgcaag tgcgcaacat cgccgaggtc accaccgccg tcgccaaagg
     5041 cgacctctcg aaaaaaatca cggttgacgt acgcggcgag atcgccgagc tgaagcgcac
     5101 cgtcaacacg atggtcgatc agctcaacgc gttagcctcg gaagtcaccc gcgtcgcccg
     5161 cgaagtcggc tccgaaggca aactcggcgg ccaggcgcag gtgcaaggcg ccgccggcac
     5221 ctggaaggat ctcaccgact cggtcaacat gatggccggc aacctcacct cgcaggtgcg
     5281 caacatcgcc gacgtaacga ccgccgtcgc caacggcgac ctctccaaga aaatcaccgt
     5341 cgacgtacgc ggcgaaattc tcgagctgaa aaataccatc aacacgatgg tcgatcagct
     5401 caacaccttt gccaatgagg tcacccgcgt tgcgagggaa gtcggtaccg a
//
LOCUS       DASUCJ010001375         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_426989, whole genome shotgun sequence.
ACCESSION   DASUCJ010001375 DASUCJ010000000
VERSION     DASUCJ010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426989"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFU90_16020"
     CDS             complement(<1..961)
                     /locus_tag="VFU90_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5481354.1"
                     /translation="MWRFDPRVPGAADVPTCCGVPNRGAAVYHGKVYVGTLDGRLIAL
                     DAASGKVVWSVVTVPANHRYTSTGAPRVARGKVFIGNAGADFGARGYVSAYDAETGRL
                     LWRFYTVPGDPRAKPDGAASDRVLATRARPTWSGDGYRYGGGGGTVWNALVYDPDFNQ
                     IYLGTGNGYPWNRSYRSDGRGDNLFIASIVALDADTGRYKWHYQESPGDSWDYDSIED
                     ITLVDLPIGAQVRKVLLHAPKNGFFYVIDRSTGKLLSAAPYVSGVNWATRIDPTTGRP
                     VINPEAEYTDRPWTGQPSGGGAHNWNPMAFSPQTGLAYFAASET"
BASE COUNT          159 a          341 c          339 g          168 t
ORIGIN      
        1 gcgtctcgct cgcggcgaaa tacgcgaggc cggtctgcgg gctgaacgcc atgggattcc
       61 agttgtgcgc gccgccgccg ctcggctgtc cggtccaagg tcgatccgtg tattcggctt
      121 cggggttgat cacgggccgc cccgtcgtcg gatcgatgcg cgtcgcccag ttgactccgg
      181 agacgtacgg cgccgcggag aggagctttc cggtgctgcg atcgatgacg tagaagaagc
      241 cgttcttcgg cgcgtgcagg agcactttgc gcacctgcgc accgatcggc aggtcgacga
      301 gcgtgatgtc ctcgatcgag tcgtaatccc aggagtctcc ggggctctcc tggtagtgcc
      361 acttgtaccg tccggtgtcg gcgtccaggg cgacgatcga ggcgatgaac aaattgtcgc
      421 cgcgaccgtc cgagcggtag ctgcggttcc acggatagcc gttgcccgtc ccgagataga
      481 tttgattgaa atcggggtcg tagacgagcg cgttccacac cgtcccgccc ccgccgccat
      541 atcgatagcc atcgccggac caggtcggcc gtgcccgggt tgccagcacg cgatccgagg
      601 ccgccccgtc gggctttgcg cggggatcgc cgggcacggt gtagaagcgc cagaggagcc
      661 ggccggtctc ggcgtcgtag gcgctcacgt agccgcgcgc gccgaaatcc gcacccgcat
      721 tgccgatgaa caccttgccg cgcgcgacgc gcggcgcacc ggtgctcgtg tagcgatgat
      781 tggcgggaac ggtgacgacg ctccacacca cctttccgct cgccgcatcg agcgcgatca
      841 gacggccatc gagcgtgccc acgtagacct tgccgtggta caccgccgcg ccgcgattgg
      901 gaacaccgca gcaggtcggc acgtccgcgg ctcccggaac gcgaggatcg aatcgccaca
      961 gctcgcgacc cgttcgggca tcgagcgcga agaccttgct ccacgcc
//
LOCUS       DASUCJ010001376         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1176327, whole genome shotgun sequence.
ACCESSION   DASUCJ010001376 DASUCJ010000000
VERSION     DASUCJ010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176327"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU90_16025"
     CDS             <1..723
                     /locus_tag="VFU90_16025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5481355.1"
                     /translation="GLALVAAIGAERPADPRRTFGYGRIEVLGALVNGTLLLVATVGI
                     VVLAVRRFSEPVEPHASIMAGIAAVGLALNLCAGAVLHRQAGGDLNVRAALFHVFGDA
                     LGALAVIAGGIAIAFTHRAWIDPALSLFVAVIIVAGVFRVMRDATNVLLESVPGDVDS
                     AALVAHLERIGGVTGVHDLHVWSIGSGSHALSAHVLLDDRRLSEAAEVLQEIDRCLQA
                     DFGITHATIQFECDNCPVVVKH"
     gene            1037..>1390
                     /locus_tag="VFU90_16030"
     CDS             1037..>1390
                     /locus_tag="VFU90_16030"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5481356.1"
                     /translation="MTSPNSKAVVLSIARTPFGRLGGGLAPLDATTLGAVALTAAIER
                     AGIDPTEIEHAVFGEVLQAGVGQNPARQVVFKAGLAKTVTADTVNKVCASGLLAVVSA
                     MRSINAGAHTVVAAGG"
BASE COUNT          225 a          455 c          461 g          249 t
ORIGIN      
        1 ggtctcgcgt tggtggcggc gatcggcgcg gagcggccgg cggatccgcg ccggacgttc
       61 ggctacgggc ggatcgaagt cctcggagcg ctggtcaacg gaacgctgct gctggtcgcg
      121 acggtgggga tcgtggtgct ggcggtcagg cgtttttccg agccggtcga accgcatgcc
      181 tcgattatgg ccggaatcgc ggccgtcggg ctcgcgttga acctgtgcgc gggcgccgtg
      241 ctgcaccgcc aagccggcgg cgatctcaac gtacgagcgg ccttgtttca cgtattcggc
      301 gacgccctcg gagcgctggc ggtaatcgcg ggcgggatcg cgatcgcgtt cacgcatcgc
      361 gcgtggatcg acccggcgct ctcgctcttc gtcgccgtca tcatcgtcgc cggcgttttc
      421 cgggtgatgc gcgacgctac caacgtcctg ctcgagagcg ttccgggcga cgtcgattcc
      481 gccgcgctgg tcgcacacct ggagcgaatc ggcggcgtca ccggagtgca cgacttgcac
      541 gtgtggtcga tcggcagcgg ttcgcacgcg ctttcggcgc acgtgctgct cgacgaccgg
      601 cgtttgagcg aagcggccga agtgctgcag gaaatcgacc gttgccttca ggccgacttc
      661 ggcatcacgc acgcgacgat tcagttcgag tgcgacaatt gcccggtcgt cgtcaaacac
      721 taaaccgccg cgaattccac ctctttttca tcccaagcaa aatcccggca ggcagcgttc
      781 ggttgaaggt cgtttctatc aagaacagcc ggcgcgctcg caggggtgag gggcttctcg
      841 atttttgggc aaacccggac ggttttttca aaacttcgtt ctcccaaaaa tcgagccgag
      901 cgagcggagt cacaggacgt gcggagcgag cgtccccgaa tcggcgcgcc ccgacgagcg
      961 cgcccgaacg cgaacgagcc ggcaacccag cccgacgccg ggattttcct aggcccacgc
     1021 gtaacccagt gcggctatga cttcacccaa ctccaaggcg gtcgtccttt cgatcgcccg
     1081 tacgccgttc ggaaggctcg gaggcggtct cgcgccgctc gacgccacta ccctcggtgc
     1141 ggtggcgtta accgccgcga tcgaacgcgc cggcatcgat cccaccgaaa tcgaacacgc
     1201 cgttttcggc gaagtgctcc aggccggagt cggccaaaac cccgctcgtc aagtggtgtt
     1261 caaggccggt ctcgcaaaga cggtgaccgc cgacaccgtc aacaaggtgt gtgcgtcggg
     1321 cttgctggcc gtcgtcagcg cgatgcgctc gatcaacgcg ggcgcgcata cggtggtggc
     1381 cgccggcggc
//
LOCUS       DASUCJ010001377         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_381867, whole genome shotgun sequence.
ACCESSION   DASUCJ010001377 DASUCJ010000000
VERSION     DASUCJ010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381867"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFU90_16035"
     CDS             complement(<1..225)
                     /locus_tag="VFU90_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481357.1"
                     /translation="MDVPYAMEFPDRVPKERYYDAEFFAREAEELWPRVWQMACRLEE
                     IPQPHDYVEYEFLDQSVIVLRTADGDVRAFQ"
     gene            263..>1253
                     /locus_tag="VFU90_16040"
     CDS             263..>1253
                     /locus_tag="VFU90_16040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5481358.1"
                     /translation="MSDSTVHVLVVGAGITGIYQLYRAREAGFSVQLLEAGAGVGGTW
                     FWNRYPGARFDSESYTYGYLFSRELFDEWVWQEHFAGQPEIERYLNHVVDRFDLRRHM
                     RFGAKVTAADWDAPSATWVVTTADGAVTRTRFLVAATGVLSVPIFPDVPGRERFGGEQ
                     YHTGLWPATPVDFTAKRVAVVGTGSSGVQIVPAIADDVASMTVYQRTANWCTPLNNAP
                     ITSEEQAQLRAEFESIRETLNTSPAGFLHEPHDRTTFDDTADERRAFYEKMWHSPGFS
                     KLTSNYTDLLVDERANREWCEFLAEKIRSIVEDPDTAEKLIPKEQRYGELRPPF"
BASE COUNT          210 a          421 c          420 g          202 t
ORIGIN      
        1 ctggaacgcg cgcacgtcgc cgtcggcggt gcggagcacg atcaccgact ggtcgaggaa
       61 ctcgtactcg acgtagtcgt gcggctgcgg gatctcctcg agccggcacg ccatctgcca
      121 cacccgcggc cagagctcct cggcttcgcg cgcgaagaac tcggcgtcgt agtagcgctc
      181 cttggggacg cggtcgggga attccatcgc gtacggcacg tccacgcgtc ggcatactaa
      241 ccggtcggta caatccgcgg ccgtgtccga ttcgacggtc cacgtcctcg tcgtcggtgc
      301 cggcatcacc ggcatctacc agctgtatcg cgcccgcgaa gccggctttt cggtgcagtt
      361 gctcgaggcc ggcgccggcg tcggcggcac ctggttctgg aaccgctacc cgggcgcgcg
      421 cttcgattcc gagagctaca cgtacggcta cctgttctcg cgcgagctgt tcgacgagtg
      481 ggtctggcag gagcacttcg cgggccagcc cgagatcgag cggtacctga accacgtcgt
      541 cgaccgtttc gacctgcgcc ggcacatgcg tttcggcgcc aaggtgacgg cggcggattg
      601 ggacgcgccg tcggcaacct gggtcgtcac cactgcggac ggcgcagtga cgcgcacgcg
      661 ttttctggtc gcggcgaccg gcgtgctgtc ggtgccgatc ttccccgacg ttcccgggcg
      721 ggaacgtttc ggtggcgagc agtatcacac cggcttgtgg ccggcgacgc ccgtcgactt
      781 cacggccaag cgcgtcgcgg tcgtcggtac gggatcgagc ggcgtgcaga tcgtgcccgc
      841 gatcgcggac gacgtggcgt cgatgacggt gtaccagcgc accgcgaact ggtgcacgcc
      901 gctcaacaac gcgccgatca cctcggagga acaggcacag ctgcgagccg aattcgaatc
      961 gatccgcgag acgctgaaca cgtcacccgc gggattcctg cacgagccgc acgaccgcac
     1021 gacgttcgac gacaccgccg acgaacggcg cgcgttctac gagaagatgt ggcacagccc
     1081 gggtttttcg aagctcacga gcaactacac cgacctcttg gtcgacgagc gcgccaaccg
     1141 ggagtggtgc gagttcctcg ccgagaagat ccgcagcatc gtcgaagatc ccgacacggc
     1201 ggagaagctg atcccgaaag aacagcggta cggcgagctg cggccgccgt tcg
//
LOCUS       DASUCJ010001378         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_901375, whole genome shotgun sequence.
ACCESSION   DASUCJ010001378 DASUCJ010000000
VERSION     DASUCJ010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901375"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..626
                     /locus_tag="VFU90_16045"
     CDS             60..626
                     /locus_tag="VFU90_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481359.1"
                     /translation="MEFLHRVQNERRAIGSSHARHHLRSSVVTGDSPSRRVVVGIAGA
                     HRREQRRYVRGGGPAMHDVDATATQCEREHRPQACHDHDQADGAQSRDPPDLGGQITA
                     GKATDDFADVYRNGRGPLTQPRFGGLDVVGDDAEHATLSQCGTDRGGDVGPLFGHKYD
                     HTRTPRNLSDKITPRLRKHSPAATVKPA"
BASE COUNT          201 a          333 c          321 g          147 t
ORIGIN      
        1 gatcatgtcg cccgcgatgt cgagacgatt ccacacctcc tcgcgccgag ctcgccgcca
       61 tggaatttct gcaccgcgtg caaaacgaac gtcgagccat cggttcgtcg cacgcccgac
      121 atcatttgcg ctcatcagtg gtaacggggg atagtccgtc gcgccgcgtt gtcgtcggca
      181 tcgccggcgc gcaccgtcgc gagcagcgtc gctacgtgcg cggcggcggc ccggcgatgc
      241 acgacgtgga cgccactgcg acgcagtgcg agcgcgaaca ccgaccgcaa gcttgccacg
      301 accacgacca agcggacggt gcgcagtctc gcgatccgcc cgatctcggc ggccagatca
      361 ccgccgggaa ggccaccgat gatttcgccg acgtatatcg caacgggcgt gggccgttga
      421 cgcagcctcg ctttggcggt ctcgatgttg ttggcgacga cgccgaacat gcgacgcttt
      481 ctcaatgcgg cacagatcgc ggcggcgacg tcggcccgct cttcggccac aagtacgacc
      541 atacacggac cccacgaaat ctctctgaca agattacacc gcggctgagg aaacactctc
      601 cagcagcaac cgtaaaaccg gcctagacgt gaggcggcgg gtgattcgcg cgacgcattc
      661 cgtgaacgcc gggcgcgctg actcggatcc tcgctaccgt ctcgaacacc gacttgaccg
      721 tcggcgggca gcggcgctaa caacaaatca agcggatcca gtaccgtgca ccgcatgccc
      781 atggtgcttg gtacgaagac cacggacgct ggcagaaggg aggctgcaaa ccgtgcgttt
      841 gggcgaccct caccgggcga aagaccgtaa ggacacggat gcgagtcttc gagcgcagcc
      901 cggcaaggcc gatcgcgaat tgtcgcgagc gcggacccaa gtggagaagg agcgggacga
      961 gcaatcgcaa cgagcgcgcg cccgctcgga tggcgcgagg gc
//
LOCUS       DASUCJ010001379         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_381910, whole genome shotgun sequence.
ACCESSION   DASUCJ010001379 DASUCJ010000000
VERSION     DASUCJ010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381910"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..320)
                     /locus_tag="VFU90_16050"
     CDS             complement(111..320)
                     /locus_tag="VFU90_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481360.1"
                     /translation="MHTIGSRSLAARQTTLKLILFAAAFASFLLSVYLYFVADDKTAG
                     IFVGIWVPSIQSLGALLLAGERGVR"
     gene            complement(465..968)
                     /locus_tag="VFU90_16055"
     CDS             complement(465..968)
                     /locus_tag="VFU90_16055"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU5481361.1"
                     /translation="MSRSRIARVEPGPGQESAWDYPRPPRLEVARARLRVVLGGVTIA
                     DTTAGVRILETSHPPNYYFPRADVRADALQREAGSSWCEWKGQAHYYTVRAGDRLERR
                     AAWSYAKPNPAYRELAGMVAFYPALMDACFVDDELVRAQPGGFYGGWITNDVVGPFKG
                     APGSHGW"
BASE COUNT          195 a          351 c          351 g          155 t
ORIGIN      
        1 ggtgtcgtcg cgattttgcc attccctgaa cagtgccagg ccgtacagga agacggcagt
       61 gctgactgcg acgaagatca ccgcaccgag ctcgaaaagg acggcctgac tcatcgaaca
      121 ccccgttcgc cggcaaggag caacgcccca agcgattgga tcgacggaac ccagatcccg
      181 acgaagatgc ctgcggtctt gtcgtcggcg acgaagtaga ggtacaccga aagcaagaac
      241 gacgcgaacg ctgccgcgaa caagatcagc ttcaatgtcg tctgacgcgc ggcaagcgag
      301 cgcgatccga tggtgtgcat gcgacctcct cgggtctata cggcggatca atgagtctcc
      361 ggagcgcagc cgcgaatgga tcacgccacc ggaacctcga tccgaggagg tgccacgcgg
      421 ccgggccggt taccgcgcga tgcggtgtgc cggcacgagg acgctcacca cccgtgcgag
      481 ccgggcgctc ctttgaacgg cccgacaacg tcgttcgtga tccagccgcc gtagaagccg
      541 cccggctgcg ctcgcaccaa ctcgtcatcg acgaagcagg catccatcaa ggccgggtag
      601 aacgcgacca ttccggccag ctcgcgatac gcgggattcg gtttcgcgta gctccacgcg
      661 gcgcgtcgct ccaaccggtc gcccgcgcgc accgtgtagt agtgggcctg tcccttccac
      721 tcgcaccagc tggacccggc ctcgcgttgc aacgcgtcgg cgcgcacgtc ggcgcgcggg
      781 aagtagtagt tcggcgggtg actggtctcg aggatccgga caccggcggt ggtgtcggcg
      841 atcgtgacac cgcccagcac gacacgcagt cgcgcgcgtg cgacctcgag acggggtggc
      901 cgcgggtagt cccacgccga ctcttggccg ggcccgggtt cgacgcgcgc gatccgcgat
      961 cggctcatgc aatgtctacg atgccgacgc ggcggccgga tcaacgtggc gggcagcgct
     1021 cagcggaaga gcgcgacgac ggtgccggtt cc
//
LOCUS       DASUCJ010001380         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1435839, whole genome shotgun sequence.
ACCESSION   DASUCJ010001380 DASUCJ010000000
VERSION     DASUCJ010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435839"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFU90_16060"
     CDS             <1..833
                     /locus_tag="VFU90_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5481362.1"
                     /translation="DVAAPAYFQALGMRLERGRFFGPQDVPNGTPTAVVSEALARAAW
                     PGRNPIGQQLSLGLSNAHRKWYTVVGVVADTRFRGLTQAPRPAVYVSAPGEAFVGVWL
                     AVRTRGNPRSMLPALTRIVRDIDPDAGIRKVETGAALLSAQTARARALATTLGVLAAT
                     VLLLAALGVFALLAALVRGRTHEIGVRMALGASDRNIRWLVAAHAVRMGALGAGIGLA
                     LALAGTRVLRAQLYQVTATDPLTMAAVVLVLIAAVLVAAYLPARRATRVDPMIALRAE
                     "
     gene            complement(891..1547)
                     /locus_tag="VFU90_16065"
     CDS             complement(891..1547)
                     /locus_tag="VFU90_16065"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5481363.1"
                     /translation="MRIGIIGAGHIGGNLARRLTAAGYDVSIANSRGPESLVDVASKT
                     GARPVWAKDAAREKDLVIVTIPEKDVKELPKDLFASAKADTVVVDTGNYYPKRDGKIA
                     SIEDGTTESRWVSEQLGRPVVKAFNTIYAHNIVDRPRPKGDDKRLALPVAGDDTRAKQ
                     VVMKVIDEIGFDSVDAGGLDDSWRQQPGTPVYGAELDANGVKRALSEAHHERPKEFRA
                     "
     gene            complement(1625..>1877)
                     /locus_tag="VFU90_16070"
     CDS             complement(1625..>1877)
                     /locus_tag="VFU90_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5481364.1"
                     /translation="ELRPLALATSSLPRAMFIGAVESPNASVLVAASEDSGVDAGRTL
                     KAVLADAGGRGGGSARLAQGTLPSRDALDGAMASLGGAR"
BASE COUNT          255 a          688 c          611 g          323 t
ORIGIN      
        1 gggacgttgc agcgccggcc tacttccagg cgttgggcat gcgcctcgag cgcggccggt
       61 tcttcggacc ccaggacgtg cctaacggaa cgcccacggc ggtggtcagc gaggccctgg
      121 cgcgtgcggc atggcccgga cgcaacccca ttgggcagca gctcagcctg gggctctcga
      181 acgcccatcg gaagtggtac acggtggtcg gcgtcgtggc cgacacgcgc tttcgcggcc
      241 taacgcaagc gccgaggcct gcggtatacg tctcggcgcc gggagaagcg ttcgtcggcg
      301 tctggctcgc cgttcgcacg cggggcaacc cgcgatccat gctcccggcg ctcacccgca
      361 tcgtacgcga catcgatccc gacgccggca tccgcaaggt cgagaccggg gccgcgcttt
      421 tgtccgccca aacggcgcgc gcccgcgcgc tcgccacgac gttaggcgtt ctggccgcga
      481 ccgtcctcct gctggccgcg ttgggcgttt tcgcgctgtt ggccgcgctg gtccgcggac
      541 gcacgcacga gatcggcgtc cgcatggcac tcggcgcgtc cgaccggaac attcggtggc
      601 tggtggccgc gcacgccgta cgcatgggtg ctcttggcgc cggtatcggc ctcgcgctcg
      661 ccctcgccgg cacgcgggtt ctccgcgcgc agctctatca ggtaacggca acggatccac
      721 tcaccatggc cgcggtggtt ctcgtgctca tcgccgccgt cttggtggct gcgtacctgc
      781 cggcgcggcg cgcgacgcgt gtcgatccga tgatcgccct gcgcgcagag tagcccggtc
      841 gtcccgcggc gctccccagc gggcgccgcg gaacatcgga cactcattcg ttaggcgcgg
      901 aattccttcg gccgctcgtg gtgcgcctcg gacagcgcgc gtttgacgcc gttcgcgtcg
      961 agctccgcgc cgtacaccgg cgtgcccggc tgctgccgcc aggaatcatc caggcccccc
     1021 gcgtccacgc tgtcgaagcc gatttcgtcg atcaccttca tcaccacctg cttcgcccgc
     1081 gtatcgtcgc cggcgacagg cagcgccagc cgcttgtcgt cgcctttggg ccgcggccga
     1141 tcgacgatgt tgtgcgcata gatcgtgttg aatgccttca ccaccggacg ccccagctgc
     1201 tccgacaccc agcggctttc tgtcgtgccg tcttcgatcg atgcaatctt tccgtcgcgc
     1261 ttgggatagt agttgcccgt gtccacgacg accgtgtcgg ctttcgccga tgcgaagaga
     1321 tccttgggca gctctttcac gtctttttcg ggaatcgtca cgatgacgag gtctttctcg
     1381 cgcgccgcgt ctttcgccca cacgggtctg gcgcccgttt tcgatgcgac atcgaccagc
     1441 gattcgggac cgcgcgaatt cgcgatcgag acgtcgtacc cggcggccgt cagccgccgc
     1501 gcaaggtttc ctccgatgtg ccccgcgcca atgatgccga tcctcatagc tgcctcctgc
     1561 ggaagttgaa cacgtgccac gagcatcctt cacgggacgt gccaccacgg gcccgacccg
     1621 atgcctaacg ggcaccgccg agggatgcca tcgcaccgtc gagcgcgtcg cgcgagggaa
     1681 gggtgccctg cgccagccgc gccgatccgc ccccgcgtcc gccggcgtcg gcgagcacgg
     1741 ccttgagcgt ccgcccggcg tcgacgccgg aatcctcgga cgcggcaacg agcaccgacg
     1801 cattcggcga ctcgacggcg ccgatgaaca tcgcgcgcgg caacgacgac gtcgcaagcg
     1861 cgagcggccg cagctcg
//
LOCUS       DASUCJ010001381         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1329152, whole genome shotgun sequence.
ACCESSION   DASUCJ010001381 DASUCJ010000000
VERSION     DASUCJ010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329152"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFU90_16075"
     CDS             complement(<1..831)
                     /locus_tag="VFU90_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481365.1"
                     /translation="MYAPHTSRDVAQMLEAVGVGSLDELLAVPEALALKERLDVVPAL
                     PEQAIAWRFDHMAKKNAGAGYRSFLGAGAYRHYIPPAIAALAMRGEFLTAYTPYQAEV
                     SQGYLQAIYEWQTYVCLLTGMDIANASVYDGATALAEAAIMAINATGRHKLLVSRAVH
                     PNYRAVLRTYCDGLDVTIDEAGFTGDGVTDSDALVAALQTKEYAAVVLQSPNFFGNVD
                     APSRAAVDAARTFETVPIAVVAEALSLAALATPASWGAEIVAGEAQSFGVPVAYGGPY
                     A"
     gene            complement(839..>1236)
                     /gene="gcvH"
                     /locus_tag="VFU90_16080"
     CDS             complement(839..>1236)
                     /gene="gcvH"
                     /locus_tag="VFU90_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5481366.1"
                     /translation="QEKHDVAQPENLLYSKEHEWVKIEGDDATVGITDYAQDSLGDIV
                     YVELPKIGAKLAQFGNAGVVESVKAVSDLFTPISGEVVEVNAALADEPGAVNRAPYED
                     GWLFKLRLSDPGETANLLDAAGYEKLLGS"
BASE COUNT          200 a          402 c          412 g          222 t
ORIGIN      
        1 ggcatacggg ccgccgtaag cgaccgggac gccgaaactt tgggcttcgc cggcaacgat
       61 ctcggcgccc cacgacgccg gcgtcgcgag ggccgcgagc gacaacgctt cggcgactac
      121 ggcgatcggt acggtctcga acgtacgcgc cgcatcgacg gccgcgcgcg acggtgcgtc
      181 gacgttgccg aaaaagttcg gcgactgcag gacgaccgcc gcgtactcct tggtctgcag
      241 cgcggcgacg agtgcgtccg agtcggtgac gccgtcgccg gtgaagccgg cttcgtcgat
      301 ggtaacgtcg agaccgtcgc agtacgtgcg cagcaccgcc cggtagttgg ggtgtacggc
      361 gcgcgacacg agcagcttgt gccgcccggt ggcgttgatc gccatgatcg cggcttccgc
      421 gagcgcggtc gcgccgtcgt aaaccgacgc gttggcaatg tccattccgg tgagcagaca
      481 gacgtacgtc tgccactcgt agatcgcttg cagataaccc tgcgaaactt ccgcctgata
      541 cggcgtgtag gcggttagga attcgcctcg catcgccagg gcggcgatcg ccggcgggat
      601 gtagtggcga tacgctccgg cgccgagaaa cgagcgataa ccggcgccgg cattcttctt
      661 ggccatgtgg tcgaagcgcc acgcgatcgc ctgctccggc agcgccggca cgacgtcgag
      721 ccgttctttg agcgcgagtg cctccggaac cgcgagcagc tcgtcgagcg atcccacgcc
      781 gacggcttcg agcatttgcg cgacgtcgcg cgaagtatgg ggtgcgtaca tcggcgagtt
      841 aggaaccgag cagtttttcg tagccggcgg cgtcgagaag gttggcggtt tcgcccggat
      901 cggaaagccg gagtttgaac agccagccgt cttcgtacgg cgcacggttc acggcgcccg
      961 gctcgtcggc gagcgccgcg ttgacctcca ccacctcgcc cgatatcggc gtaaacagat
     1021 cggagacggc ctttaccgac tcgacgacgc cggcgttgcc gaactgcgcg agcttcgcgc
     1081 cgatcttcgg cagctcgacg tagacgatgt cgccgagcga atcttgcgcg tagtcggtga
     1141 ttccgaccgt cgcgtcatcg ccctcgatct tcacccattc gtgctctttg ctgtacagca
     1201 ggttttccgg ctgcgccacg tcgtgcttct cctgtc
//
LOCUS       DASUCJ010001382         1389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_702222, whole genome shotgun sequence.
ACCESSION   DASUCJ010001382 DASUCJ010000000
VERSION     DASUCJ010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702222"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFU90_16085"
     CDS             <1..869
                     /locus_tag="VFU90_16085"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5481367.1"
                     /translation="TLGPRHAVARVSTKMNFDANSTETKTYAPQGTVLSAQSKRESYN
                     GAQAAARASGVPGTTTNIPTYQGQQQTPGGRYNKSENTTNYDISESNVKHIDAPGKVE
                     STSVAVVLNSAIPAATGGAPSKTPAQPYQITPAMRRQIQNLVVAAAGLNLSAGDQVSV
                     ESIPFNPTVATEPGAVMTQVPGTMLGIPLSVVIAILVLGAIGGLGMFAMRSRRRTFKP
                     AMELPSFDSTLAEELPPFEEHPMLDGAPGIAAPIRSAADLTREQMIEYVTTVAQENPD
                     SIAKLVKLWLAE"
     gene            996..>1389
                     /locus_tag="VFU90_16090"
     CDS             996..>1389
                     /locus_tag="VFU90_16090"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481368.1"
                     /translation="MESPIVNLSDVPGDLPDFGHSAGSDFPTVDVSGPEKAAILIITL
                     GLELSATIFKFLRQDEVERIVLEIAKISTVPLEKRDSVITEAYQRAIALKYINEGGIE
                     YAKEILERSFGAGQADDMTNRLFAALKHG"
BASE COUNT          300 a          439 c          417 g          233 t
ORIGIN      
        1 agacgctcgg cccgcgccat gcggtcgcgc gcgtatcgac gaagatgaac ttcgacgcca
       61 actcgaccga gaccaagacg tatgcaccgc aaggcacggt gctttcagcg cagtccaaac
      121 gcgaatcgta caacggcgcg caggccgctg cacgtgcatc gggcgttccg ggcacgacga
      181 ccaacattcc gacctatcaa ggccagcagc aaacgccggg cggccgctac aacaagtccg
      241 agaacaccac gaactacgac atttccgagt cgaacgtcaa gcacatcgac gcgccgggca
      301 aggtggaatc gacgagcgtc gcggtcgtat tgaacagcgc cattcccgcc gcaaccggcg
      361 gtgcgccgtc gaagacaccc gcacagccgt atcagattac gccggcgatg cgccggcaga
      421 ttcagaatct cgtcgtcgcc gccgcgggct tgaatttgtc cgcaggcgat caagtctcgg
      481 ttgaatcgat tccgttcaac cccaccgtcg ctacggaacc gggcgccgtg atgacgcagg
      541 tgccgggaac gatgctcggc attccgctca gcgtcgtaat cgcgatactc gtcctcggtg
      601 cgatcggcgg cttgggaatg ttcgcgatgc gctcgcgccg ccgtacgttc aaaccggcaa
      661 tggaacttcc gtccttcgac tcgacgctcg ccgaagagtt gccgccgttc gaagagcatc
      721 ccatgctcga cggcgctccc ggcattgcgg cgccgattcg gtccgcagcg gatctcactc
      781 gcgagcaaat gatcgagtac gtaacgacgg tcgctcaaga aaacccggac agcattgcga
      841 agctggtgaa actgtggctc gccgaatgac caggaacgga caaatgcggc gcccacgggg
      901 tgcgatcgtg tcagcagtaa cattgaggcg ccatgagatc atgggggccc caacgtgttg
      961 gggggcgcgc agcctagggc ggagcgccgg ataaaatgga atcacctatc gttaatctga
     1021 gcgacgttcc cggcgatttg cccgacttcg ggcattcggc gggaagcgat ttcccgaccg
     1081 ttgacgtctc cggccccgaa aaggccgcga tcctgatcat cacgctgggc ctggagctct
     1141 cggcgacgat cttcaaattc ttgcgtcagg acgaagtcga acgcatcgtt ctcgagattg
     1201 cgaaaatctc gacggtgccg ctcgagaagc gcgattccgt cataacggaa gcgtaccagc
     1261 gcgccattgc gctcaagtac atcaacgaag gcggcatcga gtacgccaag gagattctgg
     1321 agcgttcctt cggcgccggc caagccgacg acatgacgaa ccgtctcttc gcagcgctca
     1381 agcacggca
//
LOCUS       DASUCJ010001383         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_870647, whole genome shotgun sequence.
ACCESSION   DASUCJ010001383 DASUCJ010000000
VERSION     DASUCJ010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870647"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1090
                     /locus_tag="VFU90_16095"
     CDS             <1..>1090
                     /locus_tag="VFU90_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481369.1"
                     /translation="LVYRVNRYSDPSGEPPSDLYVAGAGGGHAVRITNSSRHDTLPQW
                     SPDGRWIAYLSMPAGTSGDFSIRLVKPDGSGNHAVFETPTTIDSLAWSPDSTSIAVAS
                     RRGTSPAIGLIDVPTGAFRWLPFTLGGGAPAWHGRRLAFVGPDGAVREADLDGSNLRT
                     LPPSAVDSVVWSGDGRRLAYVARDGADAQVFVADADGTHPRNVSQLSGLNVTSVAWAP
                     DGRVFFTAAARRDPTHTELGFSLAQAALLLQAVAVAGALLPLVRRWPLPAGACTVVLT
                     AFALAMAVESDYYYYALPALATGVAADLGIRLGGERIRSGPGFYSFGFALPAVFNAFY
                     LVTTVRAGGGSSAWPWNLLLGAPLLAGAI"
BASE COUNT          132 a          393 c          358 g          207 t
ORIGIN      
        1 ctcgtttacc gcgtcaaccg ctactccgac ccgtcgggcg aaccgccgag cgatctctac
       61 gttgccggcg ccggcggcgg acatgcggta cgcattacga attcgtcccg gcacgacacg
      121 cttccgcagt ggtcgcccga cggccgttgg atcgcctacc tttcgatgcc ggcgggaacg
      181 tcgggcgact tctccatccg actggtcaag cccgacggga gcgggaatca cgccgtcttc
      241 gaaacgccga cgacgatcga ttcgctcgcg tggtcgccgg attccacctc gattgcggta
      301 gcctcccggc gcggcacgtc gcccgcgatc ggactgatcg acgtgccgac gggagcgttt
      361 cgttggctcc cgtttacgct cggcggcggc gctccggcat ggcacgggcg acggcttgcg
      421 ttcgtcggtc cggacggcgc cgttcgcgaa gccgatctcg acggcagcaa tcttcgtacg
      481 cttccgcctt ccgccgtcga ctccgtcgtc tggtcgggcg acggtcgccg tctcgcttac
      541 gtcgcccgcg acggcgccga cgcgcaggtt ttcgtcgccg acgcggacgg aacgcatccg
      601 cgcaacgtct cacagctctc gggcttgaac gtcacgagcg ttgcgtgggc gccggacgga
      661 cgcgtcttct ttaccgctgc ggcgcgacgc gaccccaccc acaccgagct gggcttctcg
      721 ctggcacagg cggcgctgct cctccaagcc gttgcggttg ccggcgcgct gttgccgctg
      781 gtgcgccggt ggccgctccc ggccggggcc tgcacggtcg tgctcacggc cttcgcgctc
      841 gccatggccg tcgaatccga ttactactat tacgcgctgc ccgcgctcgc aaccggcgtc
      901 gcggccgacc tcggcattcg tcttgggggc gagcgaattc gcagcggtcc cggcttttat
      961 tcgttcgggt tcgccctccc ggcggtattt aacgcgttct atctcgtgac gaccgtgcgc
     1021 gccggcggcg gcagcagtgc gtggccgtgg aacctcttgc tgggagctcc gctcctggcc
     1081 ggtgcaatcg
//
LOCUS       DASUCJ010001384         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1023442, whole genome shotgun sequence.
ACCESSION   DASUCJ010001384 DASUCJ010000000
VERSION     DASUCJ010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023442"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFU90_16100"
     CDS             complement(<1..1017)
                     /locus_tag="VFU90_16100"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEU5481370.1"
                     /translation="MIVTVCAAGCAYASLATAAARVPAGSTILVHGTQRGDVLVDKPL
                     TIRGAGDAVITGGTSGIVVRAANARIEGLTLRGYALSDMADNSGALVFANAPHAVAAG
                     NVIEDCAADAIRVWYSAGVLLQGNVVRGARDVFVTYSPGVTLRANRISGARYGLHDMF
                     SDRVTVTDNVFAGNEIGANFMYARKLTITGNTFAGNHGPTGYGIGLEDVDSSRIAGNR
                     ILNNHVGLNTVDSPTDPSLPDQIESNIFSHNGSALSVQSNPHALRVLGNGFVDNIEDV
                     EVSGGGTAGGVIWNENGAGNYWSVYAGYDRNGDGIGDVAFAPRAAFDTLSDAHPELQM
                     FRYSP"
     gene            complement(1014..>1282)
                     /locus_tag="VFU90_16105"
     CDS             complement(1014..>1282)
                     /locus_tag="VFU90_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481371.1"
                     /translation="VLALPAISLPAFFVFDLASWLEYAGNHLDPHAALSGAVAPWTPN
                     LFGAGGVGQFHTLAVFEPGFYLAACASIAAIGAIVYGIKEHAAA"
BASE COUNT          235 a          410 c          424 g          213 t
ORIGIN      
        1 ggggctgtaa cgaaacattt gaagctccgg atgggcatcg ctgagggtgt cgaatgcggc
       61 tctaggagcg aacgctacgt cgccgatccc gtcgccgttg cggtcgtagc cggcatacac
      121 gctccaataa ttgccggctc cgttctcatt ccaaatcacg ccgccggccg tgccgccgcc
      181 gctaacctct acgtcttcga tgttgtccac gaagccgttg ccgagcacgc gcagcgcgtg
      241 cggattcgac tgcacggaaa gcgccgatcc attgtgagag aagatgttgc tttcaatctg
      301 gtcgggcagc gacggatcgg tgggcgaatc gaccgtgttg agcccgacgt gattgttcag
      361 gatgcggttg cccgcgatcc gcgaggagtc gacgtcctcc aagccgatgc cgtagccggt
      421 cggaccgtgg ttccctgcga acgtgttgcc ggtaatcgtg agcttgcgcg catacatgaa
      481 gttggcgccg atctcgtttc cggcgaacac gttgtccgtg accgtcacgc gatcggagaa
      541 catgtcgtgc aggccgtagc gcgcgcccga gatgcggttc gcccgcagcg ttacgcccgg
      601 cgaataggtg acgaacacgt cgcgggcgcc gcgcacgacg ttgccttgca gcagaacgcc
      661 ggcgctgtac cacacccgaa ttgcgtccgc cgcgcaatcc tcgatgacgt tgcctgcggc
      721 tacggcatgc ggcgcgttcg cgaaaacgag cgcgccgctg ttgtcggcca tatcggagag
      781 cgcatagccg cgcagcgtga ggccttcgat gcgcgcgttt gccgcccgca ccacgatgcc
      841 ggacgtgccg ccggtgatga cggcgtcgcc ggcaccgcgg atggtgagcg gcttatcgac
      901 cagaacgtcg ccgcgctggg taccatgcac gaggatcgtg gaaccggcag gcacgcgcgc
      961 cgccgccgtt gccagcgatg catacgcaca cccggcggcg cagacggtga cgatcatgcg
     1021 gccgcgtgtt ccttgatgcc gtagacgatc gcgccaattg cggcgataga cgcgcacgct
     1081 gccagataga atccgggttc gaaaaccgca agcgtgtgaa actgcccgac gccgcccgcg
     1141 ccgaacagat tcggcgtcca cggtgcgacc gcgcccgaaa gcgcggcgtg cggatcgaga
     1201 tggttgcctg cgtactccag ccacgaagcc agatcgaaaa cgaagaacgc gggcaaggag
     1261 atcgccggca gggcgagcac cc
//
LOCUS       DASUCJ010001385         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_840075, whole genome shotgun sequence.
ACCESSION   DASUCJ010001385 DASUCJ010000000
VERSION     DASUCJ010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840075"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFU90_16110"
     CDS             complement(<1..921)
                     /locus_tag="VFU90_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5481372.1"
                     /translation="MNARRLRCSISLGMLLLGTAGFAAASVATIGARPVVVIPIEGTV
                     DEGMSHLVQRSVEQANADGAPAIVLDINSPGGLVDSAFSIRDALFAAREPVVAYVDGR
                     AYSAAALIALSANRIVMKPGASIGAAEPIPATAKMISALRGEFESTALRNHRSPAIAG
                     AMVDKTVDLPEYKGTGAILTLDTDDAVRAHVADATAPTLDAALAQSSLAGSPRSVQAY
                     TWGENLARFATSPQVSALLLTLGMLGLLIEMQTLHGIAGVIGVGALLLFFGTHVYAGF
                     SNSLVLVLAAAGIFGILWELHVVPGHAIPGA"
BASE COUNT          177 a          353 c          339 g          152 t
ORIGIN      
        1 cgccccgggg atcgcgtgtc cgggcacgac gtgcaactcc caaaggatgc cgaagatccc
       61 ggccgcggcg agtaccagca cgaggctgtt ggaaaacccc gcgtagacgt gggtgccgaa
      121 aaacagcagc agcgctccga ccccgatgac tccggcgatt ccgtgcagcg tctgcatctc
      181 gatcagcagc cccagcattc cgagggtcag cagcagcgcg ctgacttgcg ggctggtcgc
      241 gaaccgcgcc aagttttcgc cccacgtgta cgcctggacg ctgcgcggcg agccggccag
      301 gctcgactgc gcgagggctg cgtcgagggt cggtgcggtg gcgtcggcaa cgtgcgcccg
      361 cacggcgtcg tcggtgtcga gcgtgagaat ggcgcccgtt cctttgtatt ccggaaggtc
      421 gacggtcttg tcgaccatcg cgccggcgat cgccggcgag cgatgattgc gcagggccgt
      481 cgattcgaac tcgccgcgca gcgccgagat catcttcgcg gtcgccggaa tcggctcggc
      541 ggccccgatc gaggcacccg gcttcatcac gatgcggttt gccgacagcg cgatcagcgc
      601 cgccgccgaa tacgcgcggc cgtcgacgta ggcgaccacc ggctcgcgcg ccgcgaacag
      661 cgcgtcccgg atcgaaaacg ccgaatcgac caaaccgccg gggctattga tatcgagaac
      721 gatcgccggc gcaccatcgg cgttggcttg ttcgaccgaa cgctggacga ggtgcgacat
      781 gccctcgtcg accgtccctt cgatcggaat cacgaccacg ggccgcgcac cgatcgtggc
      841 gaccgaggca gccgcaaacc cggccgtccc taacagcagc attcccagcg aaatcgagca
      901 gcggaggcgc cgagcgttca tgcttgtagc tactcccgcc cgcctggaaa ggttacgacg
      961 gaccgggcgg ttcggccagc agctccgtca cgacctgacg gacgaggttt ccgtcggcct
     1021 c
//
LOCUS       DASUCJ010001386         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1084726, whole genome shotgun sequence.
ACCESSION   DASUCJ010001386 DASUCJ010000000
VERSION     DASUCJ010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084726"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFU90_16115"
     CDS             <1..709
                     /locus_tag="VFU90_16115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481373.1"
                     /translation="TLVVTVAVGAAAGWVNALPVVKLNLPPFITTLAMLEMALGLSFI
                     LSHGRPVALRSPDFQNTGIGSFLGGVTSALHLPSIPVPVIWLLAIIIIASTLLTRTRF
                     GRYVFAIGGNEEAARLAGIDVARVKTLVYVISGACAAIVGFLYMALFSSGSPQTGTGD
                     ELLESIAAVVVGGTSLMGGRGSIIGTFFGALLIGVLYNAMNLLNVDSYLQKVVLGAVI
                     LLAVVLDELRKRYFSRT"
     gene            complement(787..>1390)
                     /locus_tag="VFU90_16120"
     CDS             complement(787..>1390)
                     /locus_tag="VFU90_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481374.1"
                     /translation="ATRAENGAPALTAFDQAFAAVNDYSVIERVHEVKGTAQQNRTYQ
                     VWFMKPHYAKTLIQAGDGKGSGGVWAGGDQVSGHQGGILSGLHLKVSVHDPRAVSLRG
                     ITIPEGLIQNVVHKYATVPGTLTQLPGGKIGGVPTDRLDLKVGDPSSNDGVSEQILYL
                     SQETHWPIRQILYAGDQIVLDETISELKTNTGLTQSDFPF"
BASE COUNT          215 a          445 c          445 g          285 t
ORIGIN      
        1 gacgctggtc gtgacggtgg ccgttggagc cgccgccggc tgggtcaacg cgttgccggt
       61 agtcaagctc aacttgccgc cgttcatcac gacgcttgcg atgctcgaaa tggcgctcgg
      121 gctgtcgttc attctctcgc acgggcgccc ggtcgcatta cggtccccgg acttccaaaa
      181 caccggcatc ggctcgttcc tcggcggcgt cacgagcgcg ctccaccttc ccagcatccc
      241 agttccggtg atatggctgc tcgcgatcat catcatcgcc tcgacgctgc tcacccgtac
      301 gcgcttcggg cgctacgttt tcgcgatcgg cggcaacgag gaggcggcgc gcttggcagg
      361 catcgacgtc gcgcgcgtca agacgctggt ctacgtcatc agcggcgcct gcgcggcgat
      421 cgtcggcttc ttatacatgg cgctgttttc gtcgggctcg ccgcagaccg gaacgggcga
      481 cgagcttctc gagtcgatcg ccgcggtcgt cgtcggcggc accagcctca tgggcgggcg
      541 cggcagtatc atcgggacgt tcttcggcgc gctcctcatc ggcgtgctct acaacgcgat
      601 gaacctgctg aacgtcgatt cctacctgca aaaagtcgtg ctcggcgcgg tgatcctgct
      661 cgcggtcgtc ctcgacgaac tccgcaaacg ttacttttcc cgtacgtaaa attccggcgt
      721 ggttccggca aacgaaggct agcgcatcaa acgcgaattc ggcgcggtgc ctagggcgac
      781 gcgccgctag aacgggaagt cggattgcgt caggccggtg ttggttttta gctcggagat
      841 ggtctcgtcg aggacgattt gatcgcccgc gtagaggatc tggcggatcg gccagtgcgt
      901 ctcttgcgat agatagagga tctgctccga tacgccgtcg ttcgaggacg gatctccgac
      961 tttgaggtcg aggcgatcgg tcggcacgcc gccgatcttg ccgcccggga gctgggtgag
     1021 cgttcccggc acggtcgcgt acttgtgaac gacgttttga atgagtccct cggggatcgt
     1081 gatcccgcgc agcgacaccg cgcgcggatc gtggacgctc actttgaggt gaagccccga
     1141 gagaatgccg ccctgatggc cgctgacttg atcgccgccg gcccacacgc cgcccgaacc
     1201 tttcccgtcg ccggcctgaa tgagcgtctt agcatagtgc ggcttcatga accacacttg
     1261 atacgtccgg ttctgctgcg ccgtgccctt gacttcgtgt acgcgttcga tcaccgagta
     1321 atcgttgacc gcggcgaacg cctggtcgaa cgccgtcagc gcgggcgcgc cgttctcggc
     1381 gcgggtagcg
//
LOCUS       DASUCJ010001387         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_610548, whole genome shotgun sequence.
ACCESSION   DASUCJ010001387 DASUCJ010000000
VERSION     DASUCJ010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610548"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFU90_16125"
     CDS             complement(<1..214)
                     /locus_tag="VFU90_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481375.1"
                     /translation="MILGNVRASLTRDDAELALRLIGRSSRAALEEAEDALREHGVDA
                     LLDDPRLLAALVDGPSRLGMRASWPLF"
     gene            501..1250
                     /locus_tag="VFU90_16130"
     CDS             501..1250
                     /locus_tag="VFU90_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5481376.1"
                     /translation="MLYICIPAFDEAPTIGLLLWRIRKVLEEHPREYELIVLDDGSTD
                     ATTDTLKPYADVLPLTILRNDQCRGYASALDTLARTAAKRTRYPRRDAAIFMQGDFTD
                     QPQHLPELIKRFEGGADVVIAEQPPSPRVPVAVRRLRLLAPWVVRPFLKVAGVTDPFG
                     TFRLFRISVLRDVIRDAGDAPIVQWSGWAANVELLVKTTRLARRVETVTLDARYDLRP
                     RASRVRPLAAALDLYRFGWASRRRRATSPST"
     gene            1247..>2054
                     /locus_tag="VFU90_16135"
     CDS             1247..>2054
                     /locus_tag="VFU90_16135"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU5481377.1"
                     /translation="MTIRAARLRRRTATIGAVLGACAFIAAAPAAQPTSPAATQPAPS
                     AALSDSTPRPFGVGEKSTYTVAVGGAKVGSGDMHIVGIDTVDGHPTYHSLFTVDGGFL
                     MFKVHDVLESWFDTATLSSRRFVQKIHEVNYEKQRSYQIWPEKSEMQQQGYPVMPSVS
                     NPLDDGSFLYFVRTLPLEVGKTYTLNRYFRPEKNPVIVKVLRKERVTVPAGTFDAIVI
                     QPIIKSQGLFSEGGEAEIWLSDDPRHIVVQMKAKVPVLRTLDLYLQSYQPP"
BASE COUNT          367 a          697 c          639 g          351 t
ORIGIN      
        1 agaagagcgg ccacgacgcg cgcatgccta accgcgacgg accgtccacc aaggccgcga
       61 gcaggcgcgg atcgtccagg agcgcgtcga caccgtgctc ccgtaaggca tcttccgctt
      121 cctcgagcgc agcgcgcgaa gatcggccga tcagtcggag cgccagttcc gcgtcatcgc
      181 gcgtgagcga agcccgaacg ttgccgagaa tcatcttcac cctccgatgg atctcgtgta
      241 tgtcataccc ctgttcgcac ggcgcgggcc gcgggcccat ccttgtgtga cgacttaacg
      301 gcacgcgggc aattcggcca gtccacgtgc atggcggcac tctgcgcgcc cgagtgccag
      361 cgtgatgcgg aacacactcg ccgtcctttg catttcctaa gaagccgggg catcttgcgc
      421 cgtcgcctcg tgctccgatg ccgaacggtc gtgcgagtgg gaaccgcacc ggctgcttcg
      481 cggtatcatt catagagacc atgctataca tctgcatccc ggcgttcgac gaagcgccca
      541 ccattggcct gctgctctgg cgcatccgca aggtgctgga ggagcatccg cgcgaatacg
      601 agttgatcgt gctcgacgat ggcagcaccg acgccaccac cgacacgctc aagccgtacg
      661 ccgacgtgct gccgctcacg atcctccgga acgatcagtg tcgcggctac gcgagtgcgc
      721 tcgacacgtt agcccgcacg gccgccaagc ggacgcgcta tccccgccgt gacgccgcca
      781 tcttcatgca aggcgacttc accgaccagc cgcagcacct gccggagctg atcaagcgct
      841 tcgagggcgg cgccgacgtg gtcatcgcgg agcagccgcc atcgccgcgc gtccccgtgg
      901 cggtgcgtcg cctgcgcctc ctcgcgccgt gggtcgtccg accgttcctc aaggtagccg
      961 gcgtcaccga tccattcggc acgtttcgat tgttccgcat ctcggtcctg cgcgatgtca
     1021 ttcgcgatgc cggcgatgcg ccgatcgtgc agtggagcgg ttgggcggcc aacgtcgaac
     1081 tgttagtcaa gaccacccgc ctggcgcgcc gcgtggaaac cgtcacgctg gatgcgcgat
     1141 acgatctcag accgcgcgcg tcgcgcgttc ggccgctcgc ggcggcgttg gacctctacc
     1201 gcttcggttg ggcaagtcgg cgccgtcgcg cgacgagccc ttcgacatga caatccgggc
     1261 cgcgcgactc cggcgccgca ccgcgacgat cggtgcggta ctcggcgctt gcgcatttat
     1321 cgcggcggcg ccggcggcgc aaccgacgtc gcccgccgcg acgcaaccgg cgccatccgc
     1381 ggcgctcagc gactccacac cgcgtccatt cggtgtcgga gaaaagtcga cgtacacggt
     1441 ggccgttggc ggagcaaagg tgggcagcgg cgacatgcac atcgtcggca tcgacacggt
     1501 ggacggacac ccgacctacc actcgctgtt caccgtcgat ggcggcttcc tgatgttcaa
     1561 ggtgcacgac gtgctcgaaa gctggttcga caccgcgaca ctctcgtcgc gccgattcgt
     1621 ccagaaaatc cacgaagtga attacgaaaa gcagcggagc taccagatct ggcccgagaa
     1681 gtccgagatg cagcaacagg gctatccggt gatgccgagc gtttcgaatc cgttggacga
     1741 cgggtcgttc ctctactttg tgcgtacgct gccgctcgag gtgggaaaga cgtacacgct
     1801 caaccgttat ttccgccccg agaagaaccc agtaatcgtg aaggtgctgc ggaaggagcg
     1861 cgtcacggta ccggccggca cgttcgacgc aatcgtcatc caaccgatca tcaagtcgca
     1921 ggggttgttc tccgagggcg gcgaggccga gatctggctg agcgacgatc cgcggcacat
     1981 tgttgtccag atgaaagcca aggtgcccgt gttgagaacc ctcgatctct acctgcaatc
     2041 gtaccagccg ccga
//
LOCUS       DASUCJ010001388         1277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_519279, whole genome shotgun sequence.
ACCESSION   DASUCJ010001388 DASUCJ010000000
VERSION     DASUCJ010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519279"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFU90_16140"
     CDS             complement(<1..220)
                     /locus_tag="VFU90_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481378.1"
                     /translation="MALLTLAAIAIALVGATLASAAGDGAVTGVQVGCETITFTSTKD
                     LSNVIVKYEDGTYAKFDNLSGPSYDIPNN"
     gene            complement(582..>1277)
                     /locus_tag="VFU90_16145"
     CDS             complement(582..>1277)
                     /locus_tag="VFU90_16145"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5481379.1"
                     /translation="YVGQVGGITREAQDEAAAKSHERAAAAQKEGRFADEITPVSIAQ
                     RKGDPIVVDSDEGVRPGTTTDSLGKLRPAFTKDGTITAGNASQISDGGAAVIVTSRAK
                     ADELGLTPLAELIGYGMVAGPDPSLLHQPSRSIKRALESNDLSVKDVDLFELNEAFAA
                     VGIASMKDLGITDEITNVNGGAIALGHPVGMSGTRVVLTLINELRRRGGGVGAAALCG
                     GGGQGDAAIVRTL"
BASE COUNT          212 a          429 c          424 g          212 t
ORIGIN      
        1 cgttgttcgg gatgtcgtag gagggaccgc tcagattgtc gaacttcgcg taggtgccgt
       61 cctcgtactt cacgatcacg ttgctcaagt ccttcgtcga cgtgaaggtg atcgtctcgc
      121 agccgacctg cactccggtc acggcgccat ccccggcggc agaagcaagc gtcgcgccga
      181 cgagcgcgat ggcgattgcc gcgagcgtca acaacgccac ggacagaacg agcagacggc
      241 gtacgcgcgc cccaaaccgc atcttctctc ccagccagtc atccggggag cttccgcgct
      301 acaccggacc tatttggtaa tagctgggaa cactagacat ggactggggt gcggcgccat
      361 atcgggcgag agcggcaaaa ccttacgacc atcggcaaac ccgcaaaacg aactaccccc
      421 ggcgcttttg ggcggagaga ccgggggcag tacgtgtgat gagcttggga ttcatctcgg
      481 ctgactggga gggacagcca ctgccggtat cgacgcgcga gcgaaagggc tggacctttc
      541 ccggacgtcg cagccgagga attggcgtcg aaaggtgcga attacagcgt gcggacgatc
      601 gcggcgtcgc cttggccgcc gccgccgcac aacgccgcgg caccgacacc gccaccgcgc
      661 cggcgcagct cgttgatgag cgtgagcacc acccgggtgc ccgacatgcc gaccgggtgg
      721 ccgagcgcga tcgcgccgcc gttcacgttc gtgatctcgt cggtgatgcc gaggtccttc
      781 atcgacgcga tgccgaccgc ggcgaacgcc tcgttgagct cgaacaggtc gacgtccttc
      841 acgctcaggt cgttggactc gagcgcgcgc ttgatcgaac gcgacggctg gtgcaacagc
      901 gacgggtcgg gaccggcgac catgccgtag ccgatcagct ccgcgagcgg cgtgagcccg
      961 agctcgtcgg cctttgcccg gctcgtcacg atgaccgcgg cgccgccgtc ggagatctgc
     1021 gacgcgttgc cggccgtgat cgtgccgtcc ttcgtgaacg cgggccgcaa cttgccgagc
     1081 gagtcggtcg tggtgccggg tcgcacaccc tcgtcgctgt cgaccacgat cgggtcgccc
     1141 ttgcgctgcg cgatcgagac cggcgtgatc tcgtcggcga agcggccttc cttctgagcg
     1201 gcggccgcgc gttcgtgcga cttggccgca gcctcgtcct gcgcctcgcg cgtgatgccg
     1261 ccgacctgac cgacata
//
LOCUS       DASUCJ010001389         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1130477, whole genome shotgun sequence.
ACCESSION   DASUCJ010001389 DASUCJ010000000
VERSION     DASUCJ010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130477"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFU90_16150"
     CDS             complement(<1..183)
                     /locus_tag="VFU90_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481380.1"
                     /translation="MPAEAAAATAGGILLPLALAQFLASYDTSSMSVAISNIAHDLNT
                     TVTGVQTAISLFTLTMA"
     gene            722..>1146
                     /locus_tag="VFU90_16155"
     CDS             722..>1146
                     /locus_tag="VFU90_16155"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEU5481381.1"
                     /translation="MLTTETRGLSSAAPNAVPARPFPYIEDYAFLSNCHTAALVAPDG
                     SIDWLCVPAFDSPSVFASLLDRQAGNFRFGPYGVTVPAGRIYDPGTNTLVTAWQTPAG
                     WIVVRDALTMGPRRHDDAVCPHTRPPADHDADHMLVRTV"
BASE COUNT          184 a          387 c          388 g          187 t
ORIGIN      
        1 ggccatggtg agcgtgaaca gcgagatcgc cgtctggacg cccgtcactg tcgtgttgag
       61 atcgtgcgcg atgttgctga tcgcgacgct catgctcgag gtgtcgtagc tcgcgaggaa
      121 ctgggcgagt gcgagcggga gcagaatgcc gccggccgtc gccgcggcgg cttcggccgg
      181 cactgccgag cgcgccggtt ctgagcgccg gcggatgccg gaaagaatcg atgccatagc
      241 accagactcc gtgttgcggc gcttcacgcg tgcacgcatc gcagtcgcgg cctgcacgag
      301 ccgggcccgg gcgtaccact gatcttgagg gcgtgggtcg aggcatggca tggcgatgat
      361 ccgccgatca acggcgcaat gttcgccacg tcgcagtgcg gcgcacagca gcagccggcg
      421 gcggctcttc ctcagcctcc ggacacggca ccttcgcgga gaatgtgatc gaagcttgtg
      481 taccgcgcac gaagaagcat ctttgcgatc gcgaacgagg atgcctgttg ggtctgtgtt
      541 tcaacgccgc ggcgtaccga tcttcgctac cggaaacacc gaaactcgtc gttgttgcgc
      601 ggaagcatgc tcggaatagt ggacgcgacg aggcgtgcgc cgggcagtgt tacctccttt
      661 ccacgcgccg gcgcgccgcc tcgtgagcca ctccgggatg atcgccggag aaggagtgcc
      721 cgtgctgacc actgagacgc gaggactgag cagcgccgcg ccgaacgccg ttccggcgcg
      781 cccgtttccc tatatagagg attacgcctt cctttcgaac tgccacacgg cggcgctcgt
      841 cgcgcccgac ggcagcatcg actggctctg cgtaccggcg ttcgactcgc cgagcgtctt
      901 cgcgagcctg ctcgaccgcc aggcgggaaa cttccgtttc ggcccgtacg gcgtgaccgt
      961 ccccgctggg cgcatctacg acccgggcac gaacacgctg gtcaccgcct ggcagacgcc
     1021 ggccggctgg atcgtcgtgc gcgatgcgct gacgatgggg ccgcgccgcc acgacgacgc
     1081 ggtctgcccg cacacgcgcc cgccggccga ccacgacgcg gaccacatgc tcgtacgcac
     1141 cgtcga
//
LOCUS       DASUCJ010001390         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1054124, whole genome shotgun sequence.
ACCESSION   DASUCJ010001390 DASUCJ010000000
VERSION     DASUCJ010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054124"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFU90_16160"
     CDS             complement(<1..781)
                     /locus_tag="VFU90_16160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5481382.1"
                     /translation="MKRDQTAELESRFNESGIAIAPLYTADDVEASGGLAIGRPGEYP
                     FTRGIHPLMYRRQPWTMRQYTGFGNAADTNARFKYLIANGQTGLNVAFDLPTQCGLDS
                     DDAQAEGEVGRVGMAIDTLRDFEIAFDGIDLDRITVSLTINGAAAILIAMYLAMAEKR
                     GYDVRKLRGTAQNDILKEFIGRGTWIFPVQPSIRLVGDTIEYCAKRAPKYSPVSVCGY
                     HIRESGATPTQEMAYAFCIAKAYADEVIKRGLPVDEFAGRLS"
     gene            complement(778..>1016)
                     /locus_tag="VFU90_16165"
     CDS             complement(778..>1016)
                     /locus_tag="VFU90_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481383.1"
                     /translation="PEDVDVIALSSMAGSHLSFCRRLKPLLEEADLTDKLWIIGGNLP
                     AQDHGELRELGFRGIFPTSSKLDAICDFIRENAP"
BASE COUNT          162 a          335 c          329 g          190 t
ORIGIN      
        1 aggaaagccg cccggcgaac tcgtcgaccg gcagcccgcg cttgatgact tcatcggcat
       61 aggctttggc gatgcagaac gcgtaggcca tctcctgcgt cggcgtcgcg cccgattcgc
      121 ggatgtgata gccgcacacc gacaccgggc tgtacttggg cgcgcgcttc gcgcagtact
      181 cgatcgtgtc gccgacgagg cggatcgaag gctgcaccgg gaagatccac gtgccgcggc
      241 cgatgaactc cttcaggatg tcgttctgcg cggtgccgcg cagcttcctg acgtcgtagc
      301 cgcgcttctc cgccatcgcg agatacatcg cgatcaggat cgccgcggcg ccgttgatcg
      361 tcagcgacac ggtgatcctg tcgaggtcga tgccgtcgaa cgcgatctcg aagtcgcgca
      421 gcgtgtcgat cgccatgccg acgcggccga cttcaccctc cgcctgcgcg tcgtcggagt
      481 ccagcccgca ctgcgtcggc aggtcgaacg ctacgttgag gcccgtctgc ccgttggcga
      541 tcaggtactt gaagcgtgca ttggtgtcgg ccgcgttacc gaagcccgtg tactgcctca
      601 tcgtccacgg ctgcctgcgg tacatcagcg gatggatgcc gcgggtgaac ggatactctc
      661 cgggacggcc gatcgccagt ccgccgctcg cctcgacgtc gtcggccgtg tacagcggcg
      721 cgatcgcgat gccggattcg ttgaatcgcg actcgagctc ggctgtctgg tcgcgcttca
      781 tggagcgttc tctcgaatga aatcgcagat cgcatcgagc ttcgagctgg tgggaaatat
      841 ccccctgaag ccgagctcgc gcagttcgcc gtggtcctgc gccggcaggt tgccgccgat
      901 gatccacagc ttgtcggtga ggtcagcttc ctcgagcagc ggcttcaagc gccggcagaa
      961 cgacaggtgc gaacccgcca tgctggagag cgcgatcacg tcgacatcct ccggcg
//
LOCUS       DASUCJ010001391         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1360142, whole genome shotgun sequence.
ACCESSION   DASUCJ010001391 DASUCJ010000000
VERSION     DASUCJ010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360142"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFU90_16170"
     CDS             complement(<1..983)
                     /locus_tag="VFU90_16170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5481384.1"
                     /translation="MLTRLGRMSFRHRRLVVAMWIAAVVIAVVGGSAIAGETATSGTL
                     PHTDSQHAYDVLAREFPQRHGDEARIVFADVARGRSLAAAFLAQVARVSGVIEVEPLV
                     VSPSGTIAFAPITTARGSGTHPKATADEIKSLARRYERRGLDVEFAGGWFANGALPAS
                     ELFGILAAIVVLLIAFGSLIAMGLPIVTALVGVAISVAGVGVLANVFTTPDFAAQVAT
                     MIGIGVGIDYALFMVTRYRVALHRTNSPEAAVIEAINTSGRAVLFAGFTVMVSVLGIF
                     LMGLPFLHGLSVGISLAVVIAVLAALTLLPALLGFVGSNIDRLRVGRHTTAG"
BASE COUNT          226 a          361 c          311 g          130 t
ORIGIN      
        1 ccggcggtcg tgtgcctccc gacgcgcagc cggtcgatgt tagagccgac gaagcccagc
       61 aacgccggca gcaaagtgag cgcggcgagc accgcgatca cgaccgcgag cgaaatgccg
      121 accgacaaac cgtgcaagaa cggcaagccc atcaagaaga ttccgagcac cgagaccatg
      181 acggtgaacc cggcgaagag caccgcccga cccgacgtgt tgatcgcctc gatcaccgcg
      241 gcctcgggcg aattcgttcg gtgcaacgca acgcggtagc gcgtcaccat aaatagcgca
      301 tagtcgatac cgacacctat accgatcatc gtggcaacct gcgccgcgaa gtcgggcgtc
      361 gtgaacacgt tcgcgagcac gccgaccccc gcgaccgaaa ttgcgacacc gacaagcgcg
      421 gtgacgatcg gcagccccat cgcgatcagc gacccgaacg cgataagcaa cacgacgatc
      481 gccgcgagga tcccgaagag ctcacttgcg ggaagcgccc cgttggcgaa ccacccgcct
      541 gcgaactcga catcgagacc gcgacgctcg tatcgtcgcg cgaggctctt gatctcgtcg
      601 gcggtcgcct tcggatgagt ccctgaccct ctcgcagtgg tgatcggcgc gaacgcgatc
      661 gtcccgctcg gcgagaccac gagcggctca acctcgatca cacccgacac gcgcgcgact
      721 tgcgccaaga acgcagcggc caaagaacgg ccacgcgcga cgtcggcgaa cacgatacgc
      781 gcctcatcgc catggcgctg ggggaactcg cgagcaagaa cgtcgtacgc gtgttgcgaa
      841 tcggtgtgcg gcaaagtccc cgacgtcgca gtctcacccg cgatcgccga tccgccaact
      901 accgcgatca ccaccgccgc gatccacatc gcgacgacga ggcggcgatg acgaaacgac
      961 atgcggccga gccgagtcaa catgagatcc tcccggcgga tgaagggtca gtgcgctcgc
     1021 gcgctctg
//
LOCUS       DASUCJ010001392         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_45330, whole genome shotgun sequence.
ACCESSION   DASUCJ010001392 DASUCJ010000000
VERSION     DASUCJ010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45330"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFU90_16175"
     CDS             complement(<1..513)
                     /locus_tag="VFU90_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481385.1"
                     /translation="MFDPIGAAHAELAILAAAALPHGLAALSAKFAADGLALEAVVLK
                     EQLVDDFPLRELAAFDGWLATAAFRLPFDPDDVLRDGGARLAALSDAGIGYLSPLMEP
                     VLSLFAPSARFRSDVAAVRPVRVDPVFGLPLALVGVLAPEGPGHADVSPLRPLWADTA
                     IGSLGSVMRRV"
     gene            741..938
                     /locus_tag="VFU90_16180"
     CDS             741..938
                     /locus_tag="VFU90_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481386.1"
                     /translation="MREMVDRGRRRAQQAGNVRAITSPSRKVRAKRVCGNRGPNRDGS
                     GVHQLVADRLHLASASPQLIG"
     gene            complement(1085..>1600)
                     /locus_tag="VFU90_16185"
     CDS             complement(1085..>1600)
                     /locus_tag="VFU90_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEU5481387.1"
                     /translation="MMLDQQIPMEKAFFGPKLLAERMGVERLDPADIARHDPDTFAKM
                     FAGPPAIHRFPGAMAARAQALARVVAEEYGDDAATIWDGVSSGEQLLKRLSDLPGFGE
                     QKARIFLALLGKQRGVRPRGWRAAAGAFGEDGIYQSVADVTSEESLQKVRAFKKEMKA
                     AAKEAGRKSAR"
BASE COUNT          301 a          572 c          502 g          225 t
ORIGIN      
        1 aacccgccgc atcaccgacc cgagcgagcc gatcgcggtg tcggcccaca acggccgcag
       61 cggcgacacg tcggcgtgcc ccggtccctc gggcgccagc accccgacca acgccaacgg
      121 cagaccgaac accgggtcga cccgcaccgg gcgcaccgcg gcgacgtccg atcgaaaccg
      181 cgcggacggc gcgaacagtg acagcactgg ctccatcaac ggcgagaggt atccgatgcc
      241 ggcgtcactg agagcggcga gccgcgcgcc gccgtcgcgc agcacatcgt ccgggtcgaa
      301 cggcaaccga aacgcagcgg tggccagcca gccgtcaaac gcagcgagtt cgcgcaacgg
      361 aaagtcgtca acgagctgct ctttgagcac cacagcctcg agagcaagac cgtcagcggc
      421 gaacttcgcc gacaacgcgg cgagcccgtg cggcagagcg gccgcggcga gaatcgccag
      481 ttcggcgtgg gcggcgccga tcgggtcgaa caccgcgttg gtcgcgacac atcgctccct
      541 cgcaagcgcg gcgaccgaga gagcttggcc cgcaagggac ttagccgcgt cctcccagac
      601 gcgcgacatt cggcgcaact cctcgtaatc gacgacgatg cgcccgctca taaccggccc
      661 aagaacgaga tcgcgcttcc gacgtcgtgc gcaaccgtct gttcaaggtg acgcagccgg
      721 gccttctcca actcggcgtc atgcgagaaa tggttgaccg cggacgacgc agagcgcagc
      781 aggccggcaa cgtgcgagca atcacgtctc cgtcgcgaaa ggtgcgcgcg aaacgcgtct
      841 gcggcaaccg aggtccaaat cgcgacggca gcggcgtgca ccagctcgtc gccgatcgcc
      901 tccacctggc gagcgcgtcg ccgcagctca tcggctagcg catccaggct cgacacacgc
      961 caccccgaat ccctttgtca gcaggaaaga ttcgagacga acctaagcgg tggcggcaac
     1021 cgtcaagcag ccaacgaacg aggtggtgaa aaccgcgtcg ccgccctgtg gacggtcttc
     1081 gtcgtcagcg tgccgacttg cggccggcct ctttggccgc cgccttcatt tctttcttga
     1141 aggcgcgcac cttctgcagc gattcttcgc tggtgacgtc ggcgaccgac tggtagatgc
     1201 cgtcctcacc aaacgcgccc gccgccgcgc gccagccgcg gggacgcacg ccgcgttgct
     1261 ttccaagcag ggcaaggaag atccgggcct tttgctcacc gaagcccggc agatcgctga
     1321 gccgcttcag tagctgctcg cccgacgaca cgccgtccca tatcgtcgca gcgtcgtcgc
     1381 cgtactcctc cgcgaccact cgagcaagcg cttgcgcgcg tgccgccatc gcacctggaa
     1441 agcggtgaat cgctggcggg ccggcgaaca ttttcgcgaa cgtgtccgga tcgtggcggg
     1501 cgatgtctgc gggatcaagg cgttcgacgc ccatgcgctc agccagcagc ttcggcccga
     1561 agaacgcctt ctccatcggg atttgctgat cgagcatcat
//
LOCUS       DASUCJ010001393         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1436058, whole genome shotgun sequence.
ACCESSION   DASUCJ010001393 DASUCJ010000000
VERSION     DASUCJ010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436058"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFU90_16190"
     CDS             complement(<1..342)
                     /locus_tag="VFU90_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481388.1"
                     /translation="MGGNLPRVCNFATEFESRKGDFTRFSGSVAATPRRSSKRNRPTT
                     SSSLARATRPPRNIKNGAREVRTSSFARDTLRSETDKSRAKTKGVPPLVEATSARDLL
                     RIARIFVEKSEA"
     gene            562..1212
                     /locus_tag="VFU90_16195"
     CDS             562..1212
                     /locus_tag="VFU90_16195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5481389.1"
                     /translation="MNALDKLLAVARQEMNCDVRKLAVHLVSAGLPHHAMNRLRTRVL
                     IQSGVRIGQNAAIGGPLRITGPGDMGSLLSIGEGSYISGPLHIDLGASVRIGARVYIG
                     DDVKLLTGTHEIAESQQRCGDQRWAPIEIGDGTWIGSAVTILPGATIGSGAIIGAGAV
                     VTGSVPSDTLYAGVPARFVRDLEGERRGPEPRSGIRIVSAARADSVPPPARVAVSR"
     gene            1265..>1509
                     /locus_tag="VFU90_16200"
     CDS             1265..>1509
                     /locus_tag="VFU90_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5481390.1"
                     /translation="MAIGRPRVLCNFAPSLDGKIAPAHKRAPFVMSRHPEDPRRMHAL
                     RARAQAVVVGATNLRADDPDLMPCPLRVVVTRAGDQV"
BASE COUNT          240 a          496 c          501 g          272 t
ORIGIN      
        1 cgcttcagac ttctcgacga agatgcgcgc gatgcggaga aggtcccttg cggaggtcgc
       61 ctcgacgagg ggcggaacgc cttttgtctt ggctcggctt ttatccgttt cgctgcgaag
      121 agtgtcgcgc gcgaagctgg aagttcgcac ctcacgagcg ccatttttga tgtttctcgg
      181 cggccgggtg gcccgtgcca atgacgaact cgtcgttggt cggttgcgtt tcgatgaacg
      241 acgcggcgta gccgccaccg acccgctgaa gcgtgtaaaa tctccctttc gagattcgaa
      301 ttccgtggcg aaattgcata cccgtggcaa gttgccgccc aaggacgagt cctcatcccg
      361 acttccgttc cgaatgaatc tcttcgaatt tgattgcggt tcgaggcttg cccggggtag
      421 ttggcgccga tccgctagag ccatggtcca gcccttgcgt ggcgttcgcc gttccgcggc
      481 agggagccgc gtgcgaatcc catctatgcg ccttctcggc gtcgatcact ggagccggat
      541 tacccatcga gaggcccacg catgaacgcg ctcgacaagc tgctcgctgt tgcacgccag
      601 gaaatgaact gcgacgtccg caagctcgcg gttcatctcg tctcggccgg cctgccgcat
      661 cacgcgatga atcgcttgcg tacccgcgtt ctcatccagt caggcgttcg catcgggcag
      721 aacgccgcga tcggcggacc gctccgcatc accggtccag gcgacatggg ctcgctcctt
      781 tcgatcggtg aaggctcgta catcagcgga ccgctccaca tcgatctcgg cgcgagcgtc
      841 cgcatcggag cgcgcgtcta tatcggcgac gacgtgaagc tcctgacggg gacgcacgag
      901 atcgcggagt cgcagcagcg ctgcggcgat caacgctggg ctccgatcga gatcggcgac
      961 ggcacgtgga tcggatcggc ggtcacgatt cttcccgggg cgacgatcgg cagcggagcc
     1021 atcatcggcg ccggcgcggt cgtcacggga agcgtcccgt cggatacgct ttacgccggc
     1081 gtgccggcgc gcttcgtccg tgatctcgaa ggggaaaggc ggggacccga accgcggagc
     1141 gggatccgca tcgtgagcgc ggctcgcgcc gactcggtgc cgccgccggc gcgagtggcg
     1201 gtctcgcgct gaggcacggc gcgaacgaga cggaacgagg cgggatctgg aggcggcctc
     1261 tcccatggcg atcggtaggc cccgcgtcct gtgcaacttc gccccgagcc tcgacgggaa
     1321 gatcgcgccg gcgcacaagc gggcgccgtt cgtcatgtcg cgtcaccccg aagacccgcg
     1381 gcgcatgcat gcgctgcggg cccgcgcgca agccgtcgtc gtcggcgcga cgaatctccg
     1441 cgccgatgat ccggacctca tgccctgtcc cctacgcgtc gtcgtgacgc gcgcgggcga
     1501 tcaggtcga
//
LOCUS       DASUCJ010001394         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1313938, whole genome shotgun sequence.
ACCESSION   DASUCJ010001394 DASUCJ010000000
VERSION     DASUCJ010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313938"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFU90_16205"
     CDS             complement(<1..591)
                     /locus_tag="VFU90_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481391.1"
                     /translation="MQRKFEFTLSRWHRIAERVRVAAQECEKVARNAFTATTISPWNK
                     VGVEDKAAEIARRGSEALALSEAGYAAVAHIRAALALRNAQLGVSAKLSEVEAAKQLA
                     VLYKAIVDGQTADMVRPEAVRELPSVVGESESYGFGRRPAQTVTLQTADATLVASLRN
                     KLEREQLRATRLLDELADLNRDQLEIELPSDVLMIAG"
     gene            941..>1466
                     /locus_tag="VFU90_16210"
     CDS             941..>1466
                     /locus_tag="VFU90_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481392.1"
                     /translation="MLEYAADLFGRDAIVDKSHGTSRCPTRHEVAEKIDRLFEIALTQ
                     AHDAREALEKIDVLYEHEPRGPDDGTDPDPLGCLFPEAQQRDELQWCFNVRALSRKSF
                     AKCISVMRWLECAQSDAYASMECATAKDERVSAAETRRRRREAERQSKRADWAFERAR
                     QTVEALLTRDDGSKM"
BASE COUNT          264 a          467 c          476 g          259 t
ORIGIN      
        1 ccccgcaatc atcagcacgt ccgacggcaa ctcgatctcg agctggtcgc gattgaggtc
       61 ggcgagctcg tcgagcagcc gcgtggcgcg caactgctcg cgttcgagct tgttacgtag
      121 cgacgcaacc agcgtcgcat cggcggtctg aagcgtgacc gtctgcgctg gtcgacggcc
      181 aaagccgtag gattccgact caccgacgac gctcggcagt tcgcgtaccg cttcgggacg
      241 aaccatgtcg gcggtctgcc catcgacgat cgccttgtac agcaccgcga gttgcttcgc
      301 cgcttcgacc tcgctcagct tggccgacac cccgagttgc gcattcctca gcgcgagcgc
      361 agcccggatg tgcgccacgg ccgcgtatcc cgcttcggag agggccagcg cttcgctgcc
      421 gcgacgggcg atctcggctg ccttgtcctc gactccgacc ttgttccagg gcgatatggt
      481 ggtcgccgta aaggcattgc gcgcaacctt ttcgcactcc tgagcggcaa cccgaacgcg
      541 ctcggcaatc cggtgccagc gtgacagcgt gaattcgaat ttcctttgca tgtcgttact
      601 ccggtgattg cgcgccagtg cttcgatggt cagaattccg acgaccgatg gcgttcggcg
      661 acgggcccga cgctgcggga gcctggtctc gcctcgtcgg gtgcattaaa gccgagtttc
      721 attggtggtg gcgcaccgtt gtacctaagt tgacaaactg catccaacga cgcaagccgc
      781 aaccatgccg cgaaatcgtc gcccgccagc gcgtccacga cgaaagcgat cgcgaacaac
      841 accgtggacg ttgccgacgg cgcgactgct cgccttggtc ggggaatcgg gtcgagccga
      901 tgtgtggggg gccgatgccg cgcggccgcg tctgcggcaa gtgctcgaat acgccgcgga
      961 cctctttggc cgtgacgcga tcgtcgacaa gagtcacggc accagtaggt gtccgacgcg
     1021 tcacgaggtg gccgagaaga tcgatcggct cttcgaaatt gctctgaccc aggcgcacga
     1081 cgcgagggag gcgctcgaaa agatcgacgt cctgtacgag cacgagccgc gcggacccga
     1141 cgatggcacc gatcctgatc cactcgggtg tcttttcccg gaagctcagc aacgcgatga
     1201 gctgcagtgg tgcttcaatg tgcgcgccct gagccggaaa tcatttgcca agtgcataag
     1261 cgtcatgcgc tggctcgagt gtgcccaaag cgacgcgtac gcgtcgatgg agtgcgcgac
     1321 cgcgaaggac gaacgggtat ccgcggccga gacgcgacgt cgacgccgcg aggccgagcg
     1381 ccaatccaaa cgtgcggatt gggcattcga gcgcgcgcgg cagaccgtgg aggcgctgct
     1441 cacgcgggac gatggttcaa agatgg
//
LOCUS       DASUCJ010001395         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1237802, whole genome shotgun sequence.
ACCESSION   DASUCJ010001395 DASUCJ010000000
VERSION     DASUCJ010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237802"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFU90_16215"
     CDS             <1..384
                     /locus_tag="VFU90_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5481393.1"
                     /translation="STLTQRALWRFMDGPAYARHLRGSRALYRERRDAFVENLGRELP
                     WAHVRPPAAGVNVWLPLPGRISTQAAFDACAREGVLVMPAEPFYPTRSGPPALRLSFG
                     HIPPGVAADGVRRLGRGLAKLTGAG"
     gene            complement(368..865)
                     /locus_tag="VFU90_16220"
     CDS             complement(368..865)
                     /locus_tag="VFU90_16220"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5481394.1"
                     /translation="MSVLWIVLALVAIQRVVELIYAQRNAGRLLERGGIEAGASHYPL
                     FVLLHAAWLIAMAVLIPPAAPPNWWLLGLYAVLQVLRVWTVASLGGYWTTRVISLPGA
                     PLVRHGPYRFLRHPNYAIVCAEIAVLPLAFGAVELAIVFSILNAALLSWRIRVEERAL
                     ASRRP"
     gene            complement(870..>1333)
                     /locus_tag="VFU90_16225"
     CDS             complement(870..>1333)
                     /locus_tag="VFU90_16225"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEU5481395.1"
                     /translation="AAALLACDGNGPSVVAAGEYTWPQSLDVMGWDVTGCGLKAIFSR
                     DIPNLVTNDLHDVATAFLEDRGLTLRDVDRFICHPGGAKVVDALEIAYELAPGTLADA
                     RDVLRDYGNMSAATVMFVLEQSLDGGPAWNRALVSALGPGFTAGFTLLENA"
BASE COUNT          236 a          457 c          454 g          186 t
ORIGIN      
        1 tcgacgctca cgcagcgcgc gctgtggcgc ttcatggacg gccccgcgta cgcgcgccac
       61 ttgcgtggat cgcgcgcgct ctaccgcgaa cggcgggatg cattcgtcga gaacctcggc
      121 cgcgagctgc cgtgggcaca cgtgcgtccg ccggcggcgg gggtgaacgt ttggctgccg
      181 ctgccgggac gcatttcgac gcaagccgca ttcgacgcgt gcgcgcgcga aggcgtcctg
      241 gtaatgccgg ccgagccgtt ctatcccacg cgcagcgggc cgccggcgct gcgtttgtcg
      301 ttcgggcaca ttccgcccgg cgtcgccgcc gacggcgttc gccggctcgg ccgcggactg
      361 gcgaaactca cgggcgccgg ctagccaggg cgcgctcctc gacgcgaatc cgccacgaca
      421 gcagcgcggc gttgagaatc gagaagacga tcgcgagctc gaccgcgccg aaggcgagcg
      481 gcagaacggc gatctccgcg caaacgatcg catagttggg atgacgcaaa aaacgatacg
      541 ggccgtgccg cacgagcggc gcgcccggca acgaaatcac gcgcgtcgtc caatacccgc
      601 ccagcgacgc caccgtccag acgcgcagaa cttgcagcac ggcgtaaagt cccagcagcc
      661 accagttcgg cggggcggcg ggcggaatca acacggccat cgcaatcagc caggccgcgt
      721 ggagcagcac gaacagcgga tagtgcgacg cgccggcttc gattccgcct cgctccagca
      781 agcgccccgc gttgcgctgg gcatagatca gctctacgac gcgctggatc gccaccagcg
      841 ccagaacgat ccataggacg ctcattcggt taggcgtttt cgagcagcgt gaaaccggcc
      901 gtgaagccgg gcccaagcgc actgaccagc gcgcggttcc aagcggggcc gccatcgagc
      961 gactgctcga gcacgaacat cacggtggcc gccgacatgt tgccgtagtc gcgcaggacg
     1021 tcgcgcgcat cggccagcgt gccgggcgcg agttcgtaag cgatttcgag ggcgtcgacc
     1081 accttcgcac cgccgggatg acagatgaac cggtcgacgt cgcgaagcgt taggccgcga
     1141 tcttccaaga aggcggtcgc gacgtcgtgt aagtcgttgg tcacgaggtt cggaatgtcg
     1201 cgcgaaaaga tcgctttgag cccgcagccg gtcacgtccc atcccatcac gtcgaggctt
     1261 tgcggccagg tgtattcgcc ggcggcgacg accgacggcc cgttcccgtc gcaagcgagg
     1321 agcgccgccg cgg
//
LOCUS       DASUCJ010001396         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1176616, whole genome shotgun sequence.
ACCESSION   DASUCJ010001396 DASUCJ010000000
VERSION     DASUCJ010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176616"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFU90_16230"
     CDS             complement(<1..1178)
                     /locus_tag="VFU90_16230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5481396.1"
                     /translation="MREQRGLLLYANAGLFCDGYILSSIGIALVTLQPDFALTPAATG
                     AIAAATLFGILAGAPLFGHLTDRHGRRILMIADLALFVVACLAQAFATNVWQLVALRF
                     VLGVAIGADYPIAAALIGESVPARIRGAALNSMQVAWFLGAAVAYAAGYALLAAGPHS
                     WKWILASPAPFAAAGLLLRARASETIPWQRARDSGALQRTPFATMFDARYRGPLAFVS
                     AMWLLQVVPLFAIYAFAPAVLASLGIRNQASAAGGVAITLAFLAGSFASLPLVERWGR
                     RPLCIAGFAVAAVAFGLLALGRPELVVGSFLAYAIAIGAAAGLELTYPVELFPTAIRG
                     SATGMAAAISRIGAFAGTFALPSLLGRYGATNIVIGACGLSALGLAVALRFAPETKGA
                     P"
     gene            complement(1175..>2596)
                     /locus_tag="VFU90_16235"
     CDS             complement(1175..>2596)
                     /locus_tag="VFU90_16235"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5481397.1"
                     /translation="RQQATPYAGGLQIGYLSSIFSNFEFFGDGDASRYESLLRQYAAF
                     VRSLQDLLAGQHQRGIVLPNVESEAAAAVFAGYAQPAQSQALVPAQSRLGALSPAAAA
                     ELRSGAARIVETEVVPAFASVATYLKGAYRNGAPAGVGLAQYPGGAEYYRYLVFANTT
                     LTIAPEKLNATGMAAVEELNGKLDGIRREVGFKGSLKAFKRFLSTDQRFFVKTPSQFG
                     ERLEVYVRRAQAAVPAYFSHTPTAPYGVEPLPKELAGAQTFGYYSQPTAALPRGRYLY
                     NAWHPERTSALGAGALICHELIPGHHFQIALQQENGALPNVRRYDFSEAGFVEGWGEY
                     ASQLCWDMGVYETPYDRAGRLMQDIMVSTRLVVDTGMNALGWSRERAMQFMRDNLTLS
                     EPQIESESLRYSTDIPGQALAYKTGELTMLQIRERARRELGARFDIRKFHAWTIDSGS
                     MTLDTLREHVDYEIAEQKSEPGK"
BASE COUNT          478 a          895 c          831 g          392 t
ORIGIN      
        1 ggcgcgccct tcgtctcggg ggcaaatcga agcgcgacgg ctaagccgag cgcgctcagc
       61 ccgcacgcgc cgatcacgat gttcgtcgca ccatagcgtc cgagcagcga cggtaaggcg
      121 aacgttccgg cgaacgcgcc gattcggctg atcgccgcgg ccattccggt cgcgctcccg
      181 cggatcgccg tgggaaacag ctctaccgga tacgtaagtt cgagaccggc ggccgcaccg
      241 atcgcgatcg catacgcaag gaacgagccg acgaccaatt ccggccgtcc gagcgccagc
      301 agtccgaatg cgacggcagc gaccgcgaag cccgcaatgc acaacggacg ccgtccccag
      361 cgttcgacca gcggcagcga ggcgaacgaa cccgccaaga acgccagcgt gatcgccact
      421 ccgccggctg ccgatgcctg attccggatc cccagcgagg ccagcaccgc cggcgcgaac
      481 gcgtaaatcg caaacagcgg aacgacctgc agcagccaca tcgccgaaac gaatgccagc
      541 ggaccgcgat aacgcgcgtc gaacatcgtc gcaaagggcg tacgctgcag cgcgccggaa
      601 tcgcgcgctc gctgccatgg gatcgtctcc gaagctctcg cgcgcagcag cagtccagcc
      661 gccgcgaacg gcgccgggct cgcgagaatc cacttccagc tgtgcgggcc ggcggccagc
      721 agtgcgtatc cggcggcgta ggcaacggcg gcgccgagaa accaggccac ctgcatgctg
      781 ttgagcgcgg cgccgcgtat ccgcgccggc accgactccc cgatcagcgc cgcggcgatc
      841 ggatagtcgg cgccgatcgc gacgccgagg acgaaccgca gcgcgaccag ttgccaaacg
      901 ttggtcgcga acgcttgcgc gagacaggcg accacgaaca gcgccaggtc cgcgatcatc
      961 aaaatacgcc ggccgtggcg gtcggtcagg tgaccgaaca gcggcgcgcc cgcgaggatc
     1021 ccgaacagcg tggccgccgc gatcgctccc gttgcggcgg gggtcagcgc aaaatccggc
     1081 tgcagcgtaa cgagcgcgat cccgatggaa ctaaggatgt atccgtcgca aaaaagaccg
     1141 gcgttggcgt acaggagcag cccgcgctgc tccctcactt tccgggttcg ctcttctgct
     1201 cggcgatctc gtagtcgacg tgttcgcgca gggtgtcgag cgtcatcgag ccgctgtcga
     1261 tcgtccacgc gtgaaactta cgaatgtcga accgcgcgcc cagctcccgg cgcgcgcgtt
     1321 cgcgaatttg cagcatcgtc agctcgccgg ttttgtaggc caatgcctga ccggggatat
     1381 cggtcgaata gcgcagcgac tcgctctcga tctgcggctc gctcagcgtc agattatcgc
     1441 gcatgaactg catcgcgcgt tcgcggctcc agccgagagc gttcattccg gtgtcgacga
     1501 ccagccgcgt ggaaaccatg atgtcctgca tcagccggcc ggcgcggtcg tacggagttt
     1561 cgtacacgcc catatcccag cacagctgcg acgcgtactc cccccagccc tcgacgaatc
     1621 cggcctccga aaaatcgtag cggcgcacgt tgggaagcgc gccgttttct tgctgcaacg
     1681 cgatttgaaa atggtgcccc gggatcaact cgtggcaaat cagcgcgccc gctcccagcg
     1741 ccgacgtccg ttccggatgc caggcattgt agagatagcg cccgcgcgga agcgccgcgg
     1801 tcggctggct gtagtagccg aaggtttgcg cgccggcgag ctctttgggc agcggctcga
     1861 cgccgtaggg tgcggtgggt gtgtgcgaaa aatacgccgg cacggcggcc tgcgcgcgcc
     1921 gtacgtacac ctcgagccgc tcgccgaact ggctcggcgt cttcacgaaa aagcgctgat
     1981 cggtcgagag aaagcgcttg aacgccttga gagacccttt gaaccccact tcgcggcgaa
     2041 tgccgtcgag tttgccgttc agttcttcca ccgccgccat ccccgtcgcg ttgagtttct
     2101 ccggcgcgat cgtgagcgtc gtattggcga acaccaaata ccggtagtac tcggccccgc
     2161 ccggatactg cgcgaggccg accccggccg gcgcgccgtt gcggtaggcg cccttcagat
     2221 acgtcgccac ggatgcaaac gcgggcacga cctccgtctc gacgatcctc gcggcaccgg
     2281 aacgcagttc cgcggcggcg gccggcgaca gtgcccccag gcggctttgc gccgggacca
     2341 gcgcctggct ctgcgccggt tgggcgtaac ctgcgaacac cgcggcggcg gcttccgact
     2401 caacgttcgg cagcacgatt ccgcgctggt gctgccccgc cagcaggtct tggagcgacc
     2461 gcacgaacgc cgcatactga cgcagcagcg attcgtagcg ggaagcgtcg ccgtcgccga
     2521 aaaattcgaa attggaaaag atgctcgaca agtaaccgat ttgcagcccg ccggcatacg
     2581 gcgtcgcttg ctgccg
//
LOCUS       DASUCJ010001397         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1207138, whole genome shotgun sequence.
ACCESSION   DASUCJ010001397 DASUCJ010000000
VERSION     DASUCJ010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207138"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFU90_16240"
     CDS             <1..844
                     /locus_tag="VFU90_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5481398.1"
                     /translation="DDLLALKVHGVGGALVPLSEVVRAGWVERDLTRYHKDLLPVAYV
                     IGDVAGHVDSPLYGMFGVRGKLAGVRFPEGGTLHEYFVRQPSDPFRSYAMKWDGEWQV
                     TYETFRDMGIAYAVGLVLIYLLVVAQFGSYLTPLVIMAPIPLTIIGVMPGHALLHAQF
                     TATSMIGMIALAGIIVRNSILLVDFVNLQLARGVPLAEAVTSSAAARAKPIALTGLAA
                     MLGAMFILDDPIFNGLAISLIFGILVSTLLTLVVIPVLYYAVNQRRARSTVPLIDGTG
                     PSAS"
     gene            complement(905..>1307)
                     /locus_tag="VFU90_16245"
     CDS             complement(905..>1307)
                     /locus_tag="VFU90_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5481399.1"
                     /translation="IGNLQPGTKINVEVWRDHKPVTLTATVGSWGDQTKVASAGSSSV
                     DSGDRLGVAVRPLTPDEKQEIGHGGLLVQNVTGPAAEAGIQPGDVIVSVGSTKVTTID
                     GLKHQIAKSGKNVALLIERNGQQIYVPVKIG"
BASE COUNT          188 a          444 c          423 g          252 t
ORIGIN      
        1 cgatgacctc ctcgcgctga aagtgcacgg cgtcggcggc gcgctggtgc cgctatccga
       61 ggtcgtgcgg gcagggtggg tcgagcgcga cctcacgcgc tatcacaagg atctcttgcc
      121 ggtcgcgtac gtgatcggcg atgtcgccgg gcacgtcgac agcccgctgt acggcatgtt
      181 cggcgtgcgc ggaaagctcg ccggcgtgag gtttcccgaa ggcggtacgc tgcacgaata
      241 cttcgtgcgc cagccttcgg atccgttccg ctcctacgcg atgaagtggg acggcgagtg
      301 gcaggtcacc tacgagacgt tccgcgacat ggggatcgcg tacgcggtcg gcctcgtgct
      361 gatctacctg ctcgtcgtcg cgcagttcgg ctcgtacctg acgccgctcg tcatcatggc
      421 gccgatcccg ctgacgatca tcggtgtgat gccggggcat gcactgctgc acgcgcagtt
      481 cacggcaacg tcgatgatcg gcatgatcgc gcttgccgga atcatcgttc gcaactcgat
      541 cctgctggtc gacttcgtga acctgcaact cgcgcgcggc gtgccgcttg ccgaagcggt
      601 cacgagctcc gccgcggcgc gcgcgaagcc gatcgcactg accggccttg ccgcgatgct
      661 cggcgcgatg ttcatcctcg acgacccgat cttcaacgga ctcgcgatct cgctgatctt
      721 cgggatcctc gtatcgacgc tcttgaccct cgtcgtgatc ccggtgcttt actacgccgt
      781 caaccaaaga cgcgcccggt cgacggtccc actcatcgac ggcacggggc catccgcgtc
      841 atgatgacga gactgcgcca cgcctgcgcg tgacggcagc aggcggggcg cagggaggac
      901 gacgtcagcc gatctttacc ggcacgtaga tctgctgccc gttgcgctcg atcaggagcg
      961 cgacgttctt gccggatttc gcgatctggt gcttcaggcc gtcgatcgtc gtcaccttgg
     1021 tcgagccgac gctcacgatc acgtcgcccg gctggatgcc ggcttcggca gccggtccgg
     1081 tcacgttctg cacgaggagc ccgccgtgcc cgatctcctg cttttcgtcg ggtgtgaggg
     1141 gacgcacggc gacgccgagc cgatcgccgc tgtcgacgct gctcgacccg gcgctcgcca
     1201 ccttcgtctg atcgccccac gagccgacgg tcgcggtgag cgtcaccggc ttgtggtcac
     1261 gccagacctc gacgttgatc ttcgtgcccg gctgcaggtt gccgatc
//
LOCUS       DASUCJ010001398         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_977915, whole genome shotgun sequence.
ACCESSION   DASUCJ010001398 DASUCJ010000000
VERSION     DASUCJ010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977915"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFU90_16250"
     CDS             complement(<1..585)
                     /locus_tag="VFU90_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481400.1"
                     /translation="MRTSNDSTRITPATWALRRACLLLVAAFALLLSVGVAGAAAAEP
                     PVWKVTATSEPTQLLTPRSEVIEMTVKGTKGSFAILSEEFGFIIEPYNVTAAELEAEL
                     EAEMGPGAVEVSGGPGDATGSKPYVLRFTGSYSNEELGLGFQAFGGDEISTKTLQAGG
                     SGGVLDLTAINVGGPTDGSTVTISDSIPSGLTAVT"
     gene            complement(582..>1103)
                     /locus_tag="VFU90_16255"
     CDS             complement(582..>1103)
                     /locus_tag="VFU90_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481401.1"
                     /translation="VGRDTNGDYDTYEWENGTVYLISSGASAEYSPFLDNSASGNDVF
                     FATTDELVSGDNDGGFDVYDARVPRPGDKPPPAAVPCTGAVCQGPPSAPNLLGVPASA
                     AFGGPGNLTPTPPAAHRKAAGKKRLRNRKLDSALKACARKHSNNRRQCERQARRHYAN
                     AKKAATRGRRGNR"
BASE COUNT          150 a          374 c          349 g          230 t
ORIGIN      
        1 cgtcaccgcc gtcagtcccg acggtatgga gtcgctgatc gtgaccgtgc tcccatccgt
       61 gggcccacca acgttgattg ctgtcagatc gagcacaccg ccgcttccgc ctgcctggag
      121 cgtttttgtt gagatttcgt cgccaccaaa cgcttggaag ccgagcccca attcctcgtt
      181 cgaatatgac ccggtgaacc tcagcacgta cggtttcgat ccggtcgcgt cgccgggtcc
      241 acctgacact tcgaccgcgc cgggacccat ttccgcttcc agttcggctt ctagttctgc
      301 cgctgtgacg ttgtacggct cgatgatgaa accgaattct tctgacaata tggcaaaaga
      361 gcctttagtg cctttgacgg tcatttcgat gacttcgctt cgcggcgtga gcaactgcgt
      421 cggttcgctc gtcgccgtca ccttccagac gggcggttcc gccgcagcgg cgccggcgac
      481 gcctaccgaa agcagcagcg cgaacgctgc gacgaggagc aggcacgcgc gacgcaatgc
      541 ccacgtggcc ggggtgatcc gggtgctgtc gttgctggtc ctcatcggtt ccccctccta
      601 ccgcgtgtgg cggccttctt cgcgttcgcg tagtgtctcc ttgcctggcg ttcgcactgt
      661 cgccggttgt tgctgtgctt gcgcgcgcag gctttcagcg cgctatcgag cttccggttg
      721 cgcaaccgct tcttgcctgc cgctttgcga tgcgcggccg gcggcgtggg cgtgaggttc
      781 cccgggccac cgaacgcggc gctggcgggc acgccgagca ggttcggcgc gctcggcggc
      841 ccctggcata ccgcgcccgt gcagggcacc gcggccggcg gcggcttgtc ccccggccgc
      901 ggcacgcgcg cgtcgtagac gtcgaagccc ccatcgttgt cgccggacac aagttcgtcg
      961 gtcgtcgcga agaacacgtc gttgccggac gcgctgttgt ccaggaaggg cgagtattcc
     1021 gcgctggcgc cggaggagat caggtacacc gtcccgttct cccactcgta ggtgtcgtag
     1081 tcgccgttcg tgtcacggcc gac
//
LOCUS       DASUCJ010001399         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1100194, whole genome shotgun sequence.
ACCESSION   DASUCJ010001399 DASUCJ010000000
VERSION     DASUCJ010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100194"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..490
                     /locus_tag="VFU90_16260"
     CDS             188..490
                     /locus_tag="VFU90_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU5481402.1"
                     /translation="MEPKMAVTATSLKLPETLKTRIARLARRAGETPHALMVKMIADQ
                     VEAAERHQALLADARKADSRMVETGHGYAAADVHTYLDAVVSGRKPPQPKPVAWSK"
     gene            501..794
                     /locus_tag="VFU90_16265"
     CDS             501..794
                     /locus_tag="VFU90_16265"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU5481403.1"
                     /translation="MAQVVYSDDAFADFERIVEFLLEQGRDDAARALTQIRSAISLLE
                     VHPLIGHRVAGELRALVISYGRSGYLAVYRFEAAPDVVRILRIRHQREAGYRD"
     gene            complement(799..1401)
                     /locus_tag="VFU90_16270"
     CDS             complement(799..1401)
                     /locus_tag="VFU90_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5481404.1"
                     /translation="MHPAIRFTSIVALGVAVLVGGCATPTGTVVLLPAQDGHQSVLSV
                     KEGDHEVLLDKPYAAAETTTQGPRAYQSSKAEVERTFGSAIAAQPERLERYTLYFNEG
                     NDTLTDESKATFERVFPDIAKRPVPDIVVIGHADSTGSDPLNDKLSLQRANTIRDEMI
                     KRGIPATDVVAEGRGKREPLVKTGEGVAEARNRRVEIIVR"
     gene            complement(1433..>1679)
                     /locus_tag="VFU90_16275"
     CDS             complement(1433..>1679)
                     /locus_tag="VFU90_16275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU5481405.1"
                     /translation="SDNSLLSAGPNSELALDQYSFDPTTNEGRFDTKLSKGTLAVVSG
                     RIAKQSPKAMTVRTPGATLGVRGTEFVVSADDGRSVR"
BASE COUNT          267 a          542 c          564 g          306 t
ORIGIN      
        1 gtggcaaggc tcggcaccga cgtcgccgcc ttcaccgccg acgccgtgca gagcgacgac
       61 atcacgatgc tggcgctgtg ctggaagggg gctgagcact gagcgccttg ggtgcgcggg
      121 gcttcgagtc ttccagccga ccgttgcgat cgtagccgtc aagtattacg atatgactac
      181 ttgtaccgtg gagccgaaga tggccgtcac cgccaccagc ctgaagttgc cggaaaccct
      241 gaagacgcgc atcgcgcgcc tcgcgcggcg cgcgggtgag acgccgcatg cgttgatggt
      301 gaagatgatc gccgaccagg tggaggcggc ggagcgtcat caggcgctgc ttgccgacgc
      361 tcgcaaggcc gattcccgca tggtcgaaac cgggcatggg tacgcggcag ccgacgtgca
      421 cacgtatctc gacgccgtcg tctcggggcg caagcctcca cagccgaaac ccgttgcatg
      481 gagcaagtaa tctacgttgc gtggcgcaag tagtctattc agatgacgcg ttcgccgatt
      541 tcgagcggat cgtcgagttc ctgctggaac agggccgcga cgatgccgct cgcgcgctga
      601 cgcagatccg cagcgcgatc tcgctcctcg aagtccatcc gttgatcgga caccgcgtcg
      661 caggtgagct tcgcgcgctc gtcatctcgt acggacgcag cggttatctc gcggtgtacc
      721 gtttcgaagc ggcacccgac gttgtccgca tcctgcggat tcgccaccag cgcgaggccg
      781 gataccgcga ctgagcgact acctgacgat aatttcgacg cggcgattgc gcgcctcggc
      841 gacgccctcg ccggtcttca ccagcggctc gcgcttgccg cggccttcgg cgacgacgtc
      901 ggtggcggga ataccgcgct tgatcatctc gtcgcggatc gtgttcgcgc gctggagcga
      961 cagcttgtcg ttcaacggat cgctgccggt gctgtcggca tggccgatca ccacgatgtc
     1021 gggcaccggg cgcttcgcga tgtcggggaa cacgcgctcg aacgttgcct tcgattcgtc
     1081 ggtcagcgtg tcgttgcctt cgttgaagta gagcgtgtag cgctcgaggc gctccggctg
     1141 cgcggcgatg gcgctgccga aggtcctctc gacctcggct ttgctcgact ggtacgcgcg
     1201 cgggccctgc gtcgtcgtct ccgcggccgc atagggcttg tcgagcagca cctcgtggtc
     1261 gccttccttc accgacagca ccgattgatg cccgtcctga gcaggcagca gcacgacggt
     1321 gccggttggc gtcgcgcagc cgccgacgag aacggcaacg cccagcgcaa cgatcgaggt
     1381 gaagcggatc gcggggtgca tgagttggag gcggtgtgcg cgctgaaccg cctcagcgca
     1441 ccgagcgacc gtcatcggca ctcaccacga actcggtgcc gcggacacct aacgtggcgc
     1501 ccggcgtacg cacggtcatg gctttgggcg attgcttcgc gatgcgcccg gacaccaccg
     1561 cgagcgtgcc cttcgagagc ttcgtatcga agcggccttc gttggtcgtc gggtcgaagc
     1621 tgtactggtc gagcgcgagc tcgctgtttg gcccggcgga aagcagcgag ttgtcactc
//
LOCUS       DASUCJ010001400         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_412019, whole genome shotgun sequence.
ACCESSION   DASUCJ010001400 DASUCJ010000000
VERSION     DASUCJ010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412019"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFU90_16280"
     CDS             complement(<1..781)
                     /locus_tag="VFU90_16280"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5481406.1"
                     /translation="MGNYIKHVVIIVQENRSFDNLFAAFPGADGQTYGKNSMGQQVAL
                     KQIGLLSKLNLDNSHAAYDVDYDNGKMDGWNLVYVNDQPCATCAYAYVNPKKIVPYWT
                     LAQQYALADHMFATESSGSFNAHQDLIRGDATLNSTQTLIDFPSHGPWGCDAQQGTTV
                     PLLTSQGKYVMNGPFPCLAYETLRDLLDAKSVSWKYYAPPLFTGGLAGAYWDGFDAIQ
                     AVRDGPEWQTNISSPEKNVFKDISHGTLADVSWVIPDGANSD"
     gene            1042..>2823
                     /locus_tag="VFU90_16285"
     CDS             1042..>2823
                     /locus_tag="VFU90_16285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5481407.1"
                     /translation="MHRDEPSLTLREAACADCSEVFRRLESGPQGLTPGEAARRRTVF
                     GANAFGERKVRASAVLARQLRNPLLILLIVTALTSLAVGEHADAYIIVAIVALSIGLG
                     FFNEYRSERAMADLTRRVRHRTIALRGGEPLAVDVTDLVPGDVVRISVGDIVPADLRL
                     CEVNDVECDQAVLTGESLPAEKSTAPAGASTPLTEAAGCAFMGTTVKSGAATGVVVAT
                     GRATAFGEIAARLAERPPETAFQLGLRQFSGLLVRVTVVLTVSIFALNALLHHPLLDS
                     LLFSLTIAVGLTPQLLPAIVTVSLATGAERLAKRAVIVKRLVSIEDFGNIDVLFTDKT
                     GTLTQGTVAFRAPLDAAGEYSEDVLRLGLACTDVSIRDGQIVGGTPLDTALWESALAR
                     GIEPSEYKRVAEAPFDYDRKRMSVLVAGASGERTLIVKGAPESVLACCTNDSPQLRAI
                     ADGLFAAGERVVAVATRRAFAGTLLAAGDERDLELRGFLTFADPVKEDAAAGIERLRR
                     LGVEVRIVTGDNERVAEKVCTDLGIAVAGRLNGTEIDALSDPELEARLADTTIFARVT
                     PDQKSRIIRLQRKLGSDVGFLGDGVNDA"
BASE COUNT          477 a          926 c          926 g          494 t
ORIGIN      
        1 gatccgaatt cgcaccgtcc gggatcaccc acgagacgtc ggccagcgtt ccgtgcgaga
       61 tgtccttgaa cacgttcttt tccggcgacg agatgttcgt ttgccattcg gggccgtccc
      121 gaaccgcttg gatcgcgtcg aacccgtccc aatacgcacc cgccaacccg ccggtgaaca
      181 gcggcggcgc atagtacttc cagcttacgc ttttcgcgtc gagcagatcc cgcagcgtct
      241 cgtacgccag gcacgggaac ggtccgttca tgacgtattt gccctgcgac gtcagcagcg
      301 gtaccgtggt gccctgctgc gcgtcgcacc cccacggccc gtgagacggg aagtcgatca
      361 gcgtctgcgt gctattcagc gtcgcatcgc cccggatcag gtcttgatgc gcgttaaagc
      421 ttccgctgct ttcggtcgcg aacatgtggt cggcgagcgc gtactgctgg gcgagcgtcc
      481 agtacggcac gatctttttc ggattgacgt aggcgtacgc gcaggtggcg cagggctgat
      541 cgttgacgta gaccaaattc cagccgtcca tcttgccgtt gtcgtagtcc acgtcgtagg
      601 cggcgtggct gttgtcgaga ttgagcttgc tgaggaggcc gatctgcttt agcgcgacct
      661 gctgacccat gctgttcttt ccataggtct gcccgtcggc gcccggaaag gcggcaaaca
      721 ggttgtcgaa gctgcggttc tcttggacga tgatgacgac gtgcttgatg tagttaccca
      781 ccgagcgatg gcgaaatgcg gaagaggact gcgtagctac tccgagcggg ctcggaacgg
      841 ggctgctgcc ggcgccgcag gatgcgagcg ccgcagctaa acaaagcgaa agataattac
      901 gtacgtgcag cataggctgc tgaaattcgg cgcgtccaac gcgcgctacc cctcaaaacc
      961 ttgccagggc gagaaaaatg acccgtcggc cccacggcaa gcgggacgtt ttgcccatcg
     1021 aagcgccgaa agagcatcgc aatgcatcgc gacgaaccat cgctgacgct gcgcgaagcg
     1081 gcctgcgccg actgctcgga agtcttccgc cgtctcgaaa gcggcccgca aggcctcacg
     1141 ccgggcgaag ccgcgcggcg gcgaacggtg ttcggggcca acgccttcgg cgagcggaaa
     1201 gtacgggcga gcgcggtgct cgcgcgccaa ctgcgaaacc cgctgctcat tctgctgatc
     1261 gtcaccgcgc tgacgtcgct ggcggtcgga gagcacgccg acgcctacat catcgtcgca
     1321 atcgtcgcgc tcagcatcgg cctcggcttt ttcaacgaat accgctcgga acgcgcgatg
     1381 gcggatctca cgcgccgggt tcggcaccgc acgatcgcgc tgcgcggcgg cgaaccgctc
     1441 gccgtggacg tcaccgatct cgtccccggc gacgtggtgc gcatctcggt cggcgacatc
     1501 gtgcccgccg atctgcgcct gtgcgaagtc aacgacgtcg agtgcgatca agcggtgctg
     1561 accggggagt cgctgccggc tgaaaaatca accgctcccg ccggcgcgtc cacgccgctg
     1621 accgaagcgg ccggctgcgc gttcatgggg acgacggtca agtccggcgc cgcgaccggc
     1681 gtggtggtgg ccaccggccg cgcgaccgcg ttcggcgaga tcgcggcacg attggcggag
     1741 cgaccgccgg aaacggcgtt tcagctcggc ttgcggcagt tctcgggact gctggtgcgc
     1801 gtcaccgtgg tgctgaccgt ctcgatcttc gcgctcaacg cgctgctgca tcatccgctg
     1861 ctcgactcgc tcttgttctc gctcacgatc gccgtcgggc tgacgccgca gctgctgccg
     1921 gccatcgtga ccgtcagcct ggcaaccggc gccgaacgcc tggcgaaacg cgcggtcatc
     1981 gtcaagcgac tggtaagcat cgaggatttc ggcaacatcg acgtactctt caccgacaag
     2041 acggggacgc ttacccaagg tacggtcgct ttccgcgcgc cgctcgatgc ggccggtgag
     2101 tattcggaag acgtgctgcg tttgggcctg gcttgtaccg acgtctcgat tcgcgacggc
     2161 caaatcgtcg gcggcacgcc gctggacacc gcgctctggg agagcgcgct ggcgcgcggc
     2221 atcgagccgt ccgaatacaa gcgcgtcgcg gaggcaccgt tcgactacga tcgcaagcgg
     2281 atgtcggtgc tcgtcgccgg cgcaagcggc gaacggacgc tgatcgtcaa gggcgctccc
     2341 gagtcggtgc tggcttgctg cacgaacgac tcgccgcagc tccgcgcgat cgccgacggt
     2401 ttgttcgccg ccggggaacg ggtagtagcg gtagcgaccc gccgcgcctt tgccggtacg
     2461 ttgctcgccg ccggcgacga gcgcgacctc gaactgcgcg gcttcttgac gttcgccgac
     2521 ccggtcaaag aagacgccgc cgccggcatc gagcgtttgc ggcgcctcgg cgtggaggta
     2581 cggatcgtca ccggcgacaa cgagcgcgtc gccgaaaagg tttgtaccga cctcgggatc
     2641 gccgtcgcgg gccggctgaa cggcaccgag atcgacgcgc tcagcgaccc ggagctggaa
     2701 gcgcgactgg cggataccac catcttcgcg cgcgttacgc ccgatcaaaa gtcgcgcatc
     2761 attcgcttgc agcgcaaact aggcagcgac gtcggctttc tgggcgacgg cgtcaacgac
     2821 gcg
//
LOCUS       DASUCJ010001401         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_198320, whole genome shotgun sequence.
ACCESSION   DASUCJ010001401 DASUCJ010000000
VERSION     DASUCJ010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198320"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFU90_16290"
     CDS             <1..691
                     /locus_tag="VFU90_16290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5481408.1"
                     /translation="DQARREIPLPHIEVIYHGLDPKKYEWTTSPSDYVCFVGRFTQVK
                     GPHVAIDVAGAAGVPIRVAGEVHPQDAEFGSREMSHRLAASHVTMLGSIGIQEKVPLL
                     RDARALLAPIAWNEPFGLALIEAMLSGCPVIAFPRGSVPELVEEGVTGFVVRDATEMR
                     EIVQRGSILDEFDRERCRARATERFGRDRMVRDYERLYARAAAKGRTGARMKRGARSS
                     ATRLLPNHHEG"
     gene            720..>1506
                     /locus_tag="VFU90_16295"
     CDS             720..>1506
                     /locus_tag="VFU90_16295"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU5481409.1"
                     /translation="MQDAVGYSSIDCRHGTTLQDSMVLKHELLFLLLDAQGNVDPPGH
                     CGLGLFHHDTRMLSHYVLNVAGGTPTQLSAQVIRTFSAQIDLAIDDKVFGGDAWNVAN
                     AVHVRRELLLDEQLTERVTFTNFLTAPIEFWAALSVANDFADIFEVRGWKRKERGTFY
                     EPEVDERSIAFSYRGRDGRVIRSVVCFDVEPDDIGPDGARWRFRLEPNRPRHLEWHVI
                     PDAPPPRDEGNRHTRRDGHQSFRRRHDAIERQYDAWRDSCTRFV"
BASE COUNT          272 a          456 c          520 g          258 t
ORIGIN      
        1 cgaccaggcg cgtcgcgaaa ttccactccc gcacatcgag gtgatctacc acgggctcga
       61 cccgaagaag tacgaatgga cgacgtcgcc gagcgactat gtgtgcttcg tgggacgctt
      121 tacgcaggtg aaggggccgc acgtcgcgat cgacgtcgcc ggggccgccg gtgtgccgat
      181 ccgcgtcgca ggcgaggtcc atccgcagga cgccgaattc gggagccgtg aaatgtcgca
      241 ccgtctcgcg gcctcacatg tgacaatgct cggcagcatc gggattcagg agaaagtgcc
      301 gttgctccgc gacgcccgcg cgcttctggc gccgatcgca tggaacgagc cctttggttt
      361 ggcgttgatc gaagcgatgt tgagcggatg cccggtgatc gcgtttccgc gcggcagcgt
      421 gccggagctc gtcgaggagg gtgtgacggg attcgtggtg cgagatgcaa ccgaaatgcg
      481 cgagatcgtg cagcgcggca gcatcctcga cgagttcgat cgcgaacgat gtcgtgcgcg
      541 cgcgaccgaa cgctttggac gcgaccgcat ggtgcgcgac tacgagcgcc tgtatgcgcg
      601 cgcagccgcg aagggacgga cgggtgctcg catgaagcga ggagcccgct ccagcgccac
      661 acgtctctta ccaaaccatc atgaaggttg aaagaaagac ggagccggca ccgatctcaa
      721 tgcaggacgc agtaggatat tcgtccatcg attgccggca cgggacgaca ttgcaggaca
      781 gcatggtgct caagcacgag ctgctctttc tgctgctcga tgcacagggc aacgtcgacc
      841 cgccgggtca ctgcggcctc ggtctcttcc accacgacac gcgcatgctg agccactacg
      901 tcttgaacgt ggccggcggc acgcccacgc aactgtcggc gcaggtgatt cggacgttca
      961 gtgcgcaaat cgatctggcc attgacgaca aggtgttcgg cggcgacgcc tggaacgtcg
     1021 ccaatgccgt gcacgtgcgg cgggagctgc tgctcgacga gcagctgacg gagcgcgtga
     1081 cgttcacgaa ttttctcacg gcgcccatcg agttttgggc ggcgctgtcg gtggccaacg
     1141 acttcgcgga cattttcgag gtgcgcgggt ggaagcgcaa agagcgcggc acgttttacg
     1201 aaccggaggt cgacgagcga tcgatcgcgt tttcgtatcg cggccgcgac ggtcgtgtga
     1261 tccggagtgt ggtgtgcttc gacgtcgaac cggatgacat cgggccggat ggcgcgcggt
     1321 ggcggttccg gctcgagcct aaccggccgc gccatctgga gtggcacgtg attcctgacg
     1381 cgccgccgcc gcgcgacgag ggcaaccgcc atacgcggcg ggacggccat cagagcttcc
     1441 ggcgtcggca cgacgcgatc gagcggcagt acgacgcctg gcgcgactcg tgtacgcggt
     1501 tcgtga
//
LOCUS       DASUCJ010001402         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1160893, whole genome shotgun sequence.
ACCESSION   DASUCJ010001402 DASUCJ010000000
VERSION     DASUCJ010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160893"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFU90_16300"
     CDS             <1..209
                     /locus_tag="VFU90_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481410.1"
                     /translation="MAMQQKYSKITEQLTQQIRDAASKIAQQKQLKLVLTREGVGYGG
                     VDITPDVEKTLNITEKATPSPGST"
     gene            229..1266
                     /gene="lpxD"
                     /locus_tag="VFU90_16305"
     CDS             229..1266
                     /gene="lpxD"
                     /locus_tag="VFU90_16305"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU5481411.1"
                     /translation="MLGTLQQLADRVGGRVVGDGELPIARIAAVDEAVPDALTFATEE
                     RYLSAALASKAAAVLVDAAIAPPTAGKPLLVVENARLALARLLAALRPSRPQGPFRHP
                     TAAIEPDAQLAGDVYVAAHAYVGRRARIGRGSVVGSGAYVSDDASIGESSWLHPHAAV
                     MEGCRVGDRVVLHAGCVIGSEGFGWAFIDGRLERIPQVGNVVLDDDVEIGANTCVDRA
                     QTGSTRIGQGTKIDNLVQIGHNCRIGKHCALASLTGLAGSTVVGDYVKIAGQVGTRGH
                     MTIGSGSTVAGQSGVWGDVPEGATVSGNPAREHREELRREVMIRRLPKLVARVDALER
                     ALERGSESRER"
     gene            1283..2143
                     /gene="lpxC"
                     /locus_tag="VFU90_16310"
     CDS             1283..2143
                     /gene="lpxC"
                     /locus_tag="VFU90_16310"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU5481412.1"
                     /translation="MTQATLGGPARFEGIGLHTGARAVAEVSPAEPGSGIAFVLDLPN
                     RPGSTVTVPAIAENVVDTSRTTVVGLDGASVSTTEHLLSALFVLGVTNAEIRVHGPEI
                     PVCDGSAQVFLAGFDAAGVVAQDVPRATLTLAEPFVVEVADRMIALFPAPAFRVRFVA
                     DFPAPVGTQYYDDAIDAATYRTEIAAARTFGYLHEVEALRARGLALGGTLDNALVFAP
                     DGPLQPLRWHNEVVRHKVLDLIGDFALLGAWPQFEVVAIKSGHELHALTTRALRARAS
                     VPSSAQSPHR"
     gene            2151..2624
                     /gene="fabZ"
                     /locus_tag="VFU90_16315"
     CDS             2151..2624
                     /gene="fabZ"
                     /locus_tag="VFU90_16315"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5481413.1"
                     /translation="MAELDIRQILEVLPHRYPILLVDRITELEPMVRVRGYKNITYNE
                     PVFMGHFPGNPLLPGVYMLEAMAQLGGAVVLEPGEFTRKTPYLAGIDKAKFRRPVIPG
                     DRLDMEVTMLRHRRNIGWVSTVAMVDGQLACSAELMFSIASDPRMFGADSKVLHG"
     gene            2621..>2902
                     /locus_tag="VFU90_16320"
     CDS             2621..>2902
                     /locus_tag="VFU90_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481414.1"
                     /translation="MIHPTAIVHPGAEIGRHTEIGPYCIVGEHVRIGTRTVLQAHVVV
                     NGWTEIGDDCQIYPFATIGAASQDRKYGGERAVTRVGSRTVLREYVSVQR"
BASE COUNT          497 a          964 c          991 g          450 t
ORIGIN      
        1 ccatggcgat gcagcagaag tactccaaga tcaccgaaca gcttacccag caaattcgcg
       61 acgcggcgag caagatcgcc cagcagaaac agctgaagct cgtgctcacg cgggagggcg
      121 tcggatacgg cggcgtcgac attacgcccg acgtcgaaaa aacgctcaac attacggaga
      181 aagcgacccc gtccccgggc agcacctagg gagtcgaggg agcgcgcgat gcttggcacg
      241 ctgcagcagc ttgccgaccg ggtcggcgga cgcgtcgtcg gcgatggcga gcttccgatc
      301 gcgcgaattg cggccgtcga cgaagccgta cccgatgcgc tgacgtttgc gaccgaggag
      361 cgctacctca gcgccgcact cgcgagcaag gcggccgccg tactggtcga tgcggcgatc
      421 gcgccgccga cggccggcaa accgctgctg gtggtcgaga acgcccgact ggcgctggcg
      481 cggctgctgg cggcgttgcg cccgagccgg ccgcaaggcc cgttccgcca ccccaccgcg
      541 gcgatcgaac ccgatgccca gctggccggc gacgtctacg tggcggcgca cgcctacgtc
      601 gggcggcgcg cccggatcgg ccgcggaagc gtcgtcggct ccggcgcgta cgtcagcgac
      661 gacgcctcga tcggcgagtc gtcgtggctg catccgcacg ccgcggtcat ggaaggctgc
      721 cgagtcggcg atcgcgtcgt gctccatgcg ggctgcgtga tcggcagcga gggcttcggg
      781 tgggctttca tcgacggccg tctcgagcgc atcccgcagg tcggtaacgt cgttctcgac
      841 gacgacgtcg agatcggtgc gaacacctgc gtggatcgcg cgcaaacggg aagcacgcgt
      901 atcggccagg gcacgaagat cgacaacctc gtgcagatcg ggcacaactg ccggatcgga
      961 aaacactgcg cgctggcgtc gctcaccggc ttggcgggtt cgaccgtcgt cggcgactac
     1021 gtaaagatcg ccggtcaagt cggcacgcgc ggacacatga cgatcggctc cggctcgacg
     1081 gtggcggggc aatccggcgt atggggagac gttcccgaag gcgccaccgt atccggtaat
     1141 ccggcgcgcg aacaccgcga ggaactgcgc cgagaggtca tgattcgcag gctgccaaag
     1201 ctcgttgctc gtgtcgacgc cttggaacgc gcgctcgagc gcgggagcga gtcgcgagaa
     1261 cgctaagaat tccccccagc cggtgaccca agcgacgctc ggaggaccgg cgcgcttcga
     1321 agggatcggc ctgcataccg gcgcgcgcgc ggtcgccgag gtatcgcccg ccgaacccgg
     1381 cagcggcatc gcgttcgtgt tggacctgcc gaaccggccc ggatccacgg tcaccgtgcc
     1441 ggcgatcgcc gagaacgtcg tcgacacgtc gcggacgacc gtcgtcggtc tcgacggcgc
     1501 gtcggtctcg acgaccgaac atctcctgtc ggcgctgttt gtgctgggcg tgaccaacgc
     1561 cgaaatccgc gtgcacggtc ccgaaattcc ggtctgcgac ggcagcgcac aagtgtttct
     1621 cgccggtttc gatgcggccg gagtggtcgc gcaagacgtt ccgcgcgcga cgctgacgct
     1681 cgccgagccg ttcgtggtcg aagtcgccga tcgaatgatc gcgctctttc cggcgccggc
     1741 gtttcgcgtg cgattcgtgg ccgacttccc cgcgccggtc ggcacgcagt actacgacga
     1801 cgcaatcgac gccgccacct atcgcacgga gatcgccgcg gcgcgaacgt tcggttattt
     1861 acacgaggtc gaggcgctgc gcgcccgggg cttggccctg ggcggaacgc tcgacaacgc
     1921 gctcgtcttc gcacccgacg ggccgctgca gccgctgcgc tggcacaacg aggtggtgcg
     1981 ccacaaagtg ctcgacttga tcggcgactt tgcgctgctc ggcgcgtggc cgcagttcga
     2041 agtcgtggcg atcaagagcg ggcacgaact acacgcgctg acgacgcgcg cgctgcgcgc
     2101 gcgagcgtcg gtgccgtcgt cagcacagtc gccgcacagg taggaactcc gtggcagaac
     2161 tagacattcg gcagatcctc gaggtcttgc cgcaccgcta tccgattctt ttagtcgatc
     2221 ggatcaccga actcgagccg atggtgcggg tccggggcta caaaaacatc acctacaacg
     2281 agccggtgtt catggggcac tttcccggca atccgctgct gcccggagtt tacatgctcg
     2341 aggcgatggc gcaactcggc ggcgcggtcg ttctcgaacc cggcgagttc acgcgcaaaa
     2401 cgccgtatct ggccggcatc gacaaggcga agttccggcg cccggtcatt ccgggcgatc
     2461 gtctcgacat ggaggtgacg atgctgcgcc accgccgcaa catcggctgg gtttcgaccg
     2521 tcgcgatggt cgacggtcag ctggcgtgct cggcggagct gatgttttcg atcgcgtcgg
     2581 atccgcgcat gttcggcgcc gactcgaagg tgctccacgg atgatccatc cgacggcgat
     2641 cgtgcatccc ggcgccgaaa tcgggagaca caccgagatc ggcccgtact gcatcgtcgg
     2701 cgagcacgtg cgcatcggca cgcgcacggt gctgcaggcg cacgtcgtcg tcaacggctg
     2761 gacggaaatc ggcgacgact gtcagatcta tccgttcgcc accatcggcg cggcctcgca
     2821 ggaccgcaaa tacggcggcg agcgggccgt cacgcgggta ggcagccgca cggtactgcg
     2881 cgagtacgta agcgtgcagc gc
//
LOCUS       DASUCJ010001403         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_152743, whole genome shotgun sequence.
ACCESSION   DASUCJ010001403 DASUCJ010000000
VERSION     DASUCJ010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152743"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..>1037
                     /locus_tag="VFU90_16325"
     CDS             76..>1037
                     /locus_tag="VFU90_16325"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5481415.1"
                     /translation="MRRFQIAFFAAALVAFAGIAPSYAYGSTGTATSETSGTRVADAM
                     ANFGSPPSGEVPILFNDHHVYANPDTLRQGRVLGAIVSHGSLMVPLRSMFEQMGATVS
                     YDAASKSVTAQKEGAQVQVTLGKNEVIINGESRPLDVPPMMYKGTLMVPVRVISEALG
                     AYVEWEPSQHLCVVRYLPPTPVPTPAPTEAPTPVPTPTPAPTAAPVLGFVQGGATFGK
                     VYNEFSAGSRDAQGSGVGSSTSGFEGSFVIAGAYLFNPWALKADFRQDGYDTTVSQTN
                     QNCLSGGCTEFKTIDGGTAVVDAFRAKQSTLDGRLEYNIGAYPGL"
BASE COUNT          189 a          339 c          339 g          170 t
ORIGIN      
        1 ggaaaccgta gagggcacac taacaaatcc gacctgacga gcgagatctc gacggcggct
       61 ctaggaggtt ccattttgag aagattccag atagcttttt tcgcggcggc cttggtggcg
      121 ttcgcgggca tcgcccccag ctacgcgtac ggcagtaccg gaacggcaac atcggaaacg
      181 tccggcacgc gagtcgcgga tgccatggcg aacttcggct ccccgccgtc cggcgaggtg
      241 cccatcctct tcaatgacca ccacgtgtat gcgaacccgg acacgcttcg ccaaggacgc
      301 gtcctcggcg cgatcgtgtc gcacggatcg ttgatggtgc cgctgcgcag catgttcgag
      361 cagatgggcg ccaccgtgtc gtacgatgcg gcgagcaagt ccgtcactgc gcaaaaagaa
      421 ggcgcgcaag tccaggtgac cctcggcaag aacgaggtca tcatcaacgg cgagtcgcgt
      481 ccgctcgacg tgccgccgat gatgtacaag ggcacgctca tggtgccggt gcgcgtcatc
      541 tcggaagcgc tcggcgcgta cgtcgaatgg gaaccctcgc agcacctgtg cgtcgtgcgc
      601 tacctgccgc cgacgccggt cccgacgcct gcgccgaccg aagcaccgac tccggtgccg
      661 acgccgacgc cggcaccgac ggccgctccc gtcctcggct tcgtccaggg cggcgcgacg
      721 ttcggcaagg tgtacaacga gttctcggcg ggttcgcgtg acgcgcaggg ctcgggtgtc
      781 ggcagcagca cgtcgggctt cgaaggctcg ttcgtgatcg ccggcgcgta cctgttcaat
      841 ccatgggcgc tcaaggccga cttccgccaa gacggctacg acacgaccgt gtcgcagacg
      901 aatcagaact gcctgagcgg cggctgcacg gagttcaaga cgatcgacgg cggtaccgcc
      961 gtggtcgacg cgttccgcgc gaagcagtcg acgcttgacg gccggctcga gtacaacatc
     1021 ggagcgtatc cgggtct
//
LOCUS       DASUCJ010001404         1350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1207215, whole genome shotgun sequence.
ACCESSION   DASUCJ010001404 DASUCJ010000000
VERSION     DASUCJ010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207215"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFU90_16330"
     CDS             complement(<1..505)
                     /locus_tag="VFU90_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5481416.1"
                     /translation="MREGGEGHRVSLLHDGRSQLRALARAPRFSATVIAILALGIGLS
                     TAVFTVADALVLRRLPVRDQSRLVELWGEAHGRSIDHVPLDLADTRDFAQRTRAEQSV
                     AYVAYEGAWPVTVRLGDADVRLRRALVSGNYFTVLGAPPVLGRVLTPNDNLFGAAPVA
                     VISYEAWQ"
     gene            complement(1160..>1350)
                     /locus_tag="VFU90_16335"
     CDS             complement(1160..>1350)
                     /locus_tag="VFU90_16335"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU5481417.1"
                     /translation="VPSSVQTVGDEWITSGRSLALRVPSVIVDGGFNILINPEHAHFG
                     RLTVDSISVYDFDPRLLR"
BASE COUNT          250 a          419 c          441 g          240 t
ORIGIN      
        1 gttgccaggc ctcgtagctg atgacggcca ccggcgcggc gccgaagagg ttgtcgttag
       61 gcgtgagcac gcgccccaga accggcggcg cgccaaggac cgtgaagtag ttgccggata
      121 cgagggcgcg gcgcaggcgc acatcggcgt cgccgagccg gacggtgacg ggccacgcgc
      181 cttcgtacgc cacgtacgcg acgctctgct cggctcgggt acgttgggcg aagtcgcgcg
      241 tgtcggcgag gtcgagcggc acgtggtcga tcgagcggcc gtgcgcttcg ccccacaact
      301 cgacgaggcg gctctggtcg cggacgggaa gtcggcgcag cacgagcgca tcggcgacgg
      361 tgaacaccgc cgtcgataaa ccgatgccta acgcgaggat ggcgatcacg gtcgccgaga
      421 accgcggcgc gcgggccagg gcgcggagct gcgagcggcc gtcgtgcagc agactcactc
      481 gatgtccctc gccgccttca cgcatcggcc gatcgtctcc caggtgcgcg tcgtcacgtc
      541 cggaccgaac gtcttggcga tgagcctcga atttcccggc atcgcgccgc aagggcggta
      601 tcggcactga tcgcacgggt cccatgcaca atctcgttga tgcgccgggc cggcacgctc
      661 atgtccttgg acagtcgata ctgcgaaatg gccaagggcc cgagaaagtc cagcgcgagc
      721 acttcgcccg ggtgaatcgg agccaatttg cgctttgcca tcgaaacctc agtgatagtc
      781 gacgatttcc acgtcatacg cgttcccgtc cttccagcga aagcagattc gccactgatc
      841 attgatccga atgctgtgct ggccggctga tcgcttgtcg gcttcggcgt acgctcggca
      901 gcggcggcga gatcgatgcg tcgcgcacgc tatggacgat tcatgatcgc ggcttccgaa
      961 cgggcgcggt ctgccaaaac tctcgatccg cctgcaccca ctcgggcgcc ggcattctcc
     1021 agttgggccg gacggcgaat tattcgtgtt gtcgatcgaa cgtcccagtt cagctgcacg
     1081 tgagctttat taggcgcttg gccaagccgt ggcaatcatt cgatcgcttg tcagctgcaa
     1141 cgttcgttag gcggcttgct cagcggagca aacgaggatc gaagtcgtac acagagatgg
     1201 aatcgactgt aagccggcca aagtgtgcgt gctccggatt gatgagaatg ttgaatcctc
     1261 cgtcaacaat cacgctgggg actcgcaagg ccaaagagcg tccggacgtg atccactcat
     1321 cgccgacagt ctgaactgac gaagggactg
//
LOCUS       DASUCJ010001405         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1375214, whole genome shotgun sequence.
ACCESSION   DASUCJ010001405 DASUCJ010000000
VERSION     DASUCJ010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375214"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFU90_16340"
     CDS             complement(<1..838)
                     /locus_tag="VFU90_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481418.1"
                     /translation="MTPVVAGSVGAPTPDSLGALFLSRFATGSPAAFDSIDPDSLSRA
                     VVHSAAQRKLTREARTMRVVRRDAQRAVLLLTGIVKAGNGGDETNLVRHFSGFYEAAM
                     HQGSWRIVRQLPIDTLNYIHGQVLHVDLAPARDIHVEDTLSVIVGTEYGMAVRLNNDV
                     RIARLRFDGDSVAWTLGGGVLWIAAPRRGHAQLALSYTLRDASGPSRAVAASSGDSLP
                     AYGAFHNTDAWHPFFDYNSANDLGPATMTVHLPAEYYLTTTLPQRDTVIGGVRTVTGH
                     STY"
     gene            complement(918..1436)
                     /locus_tag="VFU90_16345"
     CDS             complement(918..1436)
                     /locus_tag="VFU90_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481419.1"
                     /translation="MRSGDGGRGAGVIFFNDEGNEDGGLTFNGRKTADGYTAGAGLTF
                     DQYNQDETVTLSYHDQNGAREAGLDIQDRPNVPIQAFAESAMVYRKLPDGSDKTRRFQ
                     EFRKNMIAKGEFGSISRAYIGKGAAKESIVMLSDRQGHPRIKLMVDSLGTPSLQFLDA
                     DGRVTQRLPEAR"
BASE COUNT          240 a          479 c          536 g          256 t
ORIGIN      
        1 ggtacgtgct gtggccggtc acggttcgca cgccgccgat gaccgtgtcc cgctgcggca
       61 gcgtcgtggt gagatagtac tcggccggca gatggaccgt catggtggcg gggccgaggt
      121 cattggcgct gttgtaatcg aagaacggat gccaggcgtc ggtgttgtga aaggcgccgt
      181 acgcgggcag cgagtcgccc gacgacgcag cgacggcgcg cgatggaccg ctcgcgtcgc
      241 gcagcgtgta cgacagcgcg agttgcgcat ggccgcgccg cggcgccgcg atccagagca
      301 cgccgccgcc taacgtccag gccacggagt caccatcgaa gcgcaagcgc gcgatgcgga
      361 catcgttgtt gagccggacg gccatgccgt actcggtccc gacgatcacc gacaacgtgt
      421 cttcgacgtg gatgtcgcgg gccggcgcga gatcgacgtg cagcacctgg ccgtggatgt
      481 agttcagcgt atcgatcggc agctgccgca cgatgcgcca gctgccctgg tgcatcgccg
      541 cttcgtagaa gccggagaaa tgccgtacga gattggtttc gtcgccgccg ttgcccgcct
      601 tgacgatccc cgtgagcaag agcaccgcgc gttgggcatc gcgccggacg acgcgcattg
      661 tacgtgcctc gcgggtcagc ttgcgttggg cggcgctgtg cacgaccgcg cggctcaacg
      721 aatccggatc gatgctgtcg aaggcggccg gcgagccggt ggcgaaccgg ctcaggaaga
      781 gcgcgcccag cgaatccgga gtcggcgcgc ccacgctacc cgcgacgacg ggcgtcacgc
      841 tgcgcggcgg cgtcgagcag gcggcaaccg cgagcgcgac agagacaaga gtcagacgtc
      901 gagcgaatcg catgcgatca gcgcgcctcg ggcagtcgtt gcgtgacgcg gccgtccgcg
      961 tcgaggaact ggagtgacgg cgtgccgagc gagtcgacca tgagtttgat gcgcggatgc
     1021 ccctggcgat cggacagcat cacaatggat tccttggccg cgcccttgcc gatgtatgcg
     1081 cgcgatatgc tgccgaattc gcccttggcg atcatgttct tccggaactc ctggaagcgc
     1141 cgggtcttgt ccgaaccgtc cggcagcttg cggtacacca tggcgctctc ggcgaaggcc
     1201 tgaatgggca cgttaggccg gtcctggatg tcgagcccgg cttcgcgcgc gccgttctgg
     1261 tcgtggtacg agagcgtgac agtctcgtcc tggttgtatt gatcgaacgt caggcccgct
     1321 ccggcggtgt agccgtcggc ggttttccgg ccgttgaacg tcaggccgcc gtcctcgttg
     1381 ccttcatcgt tgaagaagat gacgccagcg ccgcggccgc cgtcgccgct gcgcaaggga
     1441 tacgatttcc cgccaatgac cggatcgggc agcttgtcgt ggccggagag cgtcatccgg
     1501 agccggccgt c
//
LOCUS       DASUCJ010001406         1459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1207259, whole genome shotgun sequence.
ACCESSION   DASUCJ010001406 DASUCJ010000000
VERSION     DASUCJ010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207259"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..>1459
                     /locus_tag="VFU90_16350"
     CDS             305..>1459
                     /locus_tag="VFU90_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481420.1"
                     /translation="MIEVVASALIVGLISVGTLTGFGNAGRAVADERQRGQATLLAAQ
                     DEEGLRGMNVTKLATYGTKVHTFKEQGTNYTIESKAQFVSASEEKYTCETSGGKADYI
                     QTTSTVTWPALTTAKGKREGVSQSSIVPVPTSDSLLVNVHNQANEPVEGATVKVTGKT
                     SGAVTEQTTPASGCVIFGAMADSQVTIVVSKLGWINEKLEEQSSQEAQLSTTSLVSKT
                     FVIASPGLINAEFVSAASGGAVLGDTYHVGHTSAPEKVIGAAGTYVSTLLSSKVFPYQ
                     TPLPPPENESVYTVYAGECGQTEPATLGTGLKDGKVQVNPGATAAVKIALPAINLVVN
                     EGTIADGKAEAKLTDACGSVRTMKTTSSGALEHPYQPFGKTKLCLAQKKGT"
BASE COUNT          309 a          490 c          479 g          181 t
ORIGIN      
        1 ttaccgatac accagtcccc cggggccacg ggattgtagg cgctgaacgc cccgacgatc
       61 cttcggcggc gcgggcgtcg ggcgtggatg cgcgggcacg agcgccgcaa atcgcccgca
      121 aacacggcgc cgatcgtgcg cctagcgcac cggcgctcgc tcaaggcggg tcacgtcggt
      181 gccgacacgt acgcaggcga ctccagcctg atcgacgatg cgtactcctc ccgccctgct
      241 ccggtcatcg cgccttgccc tggcgaggct cgccgacgag gcctcagcgg agtccgggat
      301 cgcgttgatc gaagtcgtcg caagcgcgct gatcgtcggg ctgatctcgg tcgggacgct
      361 gacgggcttc ggcaacgccg ggcgggcggt cgccgacgag cgtcagcgcg gccaggcgac
      421 gctgctcgca gcgcaggacg aggaagggct ccgcgggatg aacgtcacca agctcgcgac
      481 atacggcacg aaagtgcaca cgttcaagga acaaggcacg aactacacga tcgaatcgaa
      541 ggcgcagttc gtcagcgcga gcgaagaaaa gtacacgtgc gaaacgagcg gcggcaaggc
      601 cgactacatc cagacgacct cgaccgtgac gtggccggcg ctgacgacgg cgaaaggcaa
      661 acgcgaaggc gtctcgcaat cgagcatcgt gccggtgccg acgagcgaca gcctgctcgt
      721 caacgtccac aaccaggcca acgaaccggt cgaaggcgcg accgtcaagg tcaccgggaa
      781 aacctcgggc gcggtcaccg aacagacgac gccggcgtcc ggctgcgtga tcttcggcgc
      841 gatggcggac agtcaggtga cgatcgtcgt ctcgaaactg ggctggatca acgaaaaact
      901 cgaagaacag tcttcccagg aagcgcagct gtcgacgacg tcgctcgtca gcaagacgtt
      961 cgtgatcgcc agcccgggcc tgatcaacgc cgaattcgtc agcgcggcgt caggcggcgc
     1021 ggtgctgggc gacacctacc acgtaggcca cacgagcgcg cccgaaaaag tcatcggcgc
     1081 cgcgggcacg tacgtgtcga cgctgctctc gagcaaggtc ttcccctacc agacgccgct
     1141 ccccccgccc gaaaacgaaa gcgtctacac cgtctacgcc ggcgagtgcg gccagaccga
     1201 gccggcgacc ctcggcaccg ggctgaaaga cggcaaggtc caggtgaacc caggcgccac
     1261 cgccgcggtc aagatcgcac tgccggcgat caacctcgtc gtcaacgaag gcaccatcgc
     1321 cgacggcaaa gccgaagcga agctgaccga cgcgtgcggc tcggtgcgga cgatgaaaac
     1381 aacgagcagc ggcgcgctcg aacaccccta tcagccgttc ggcaagacga agctgtgctt
     1441 ggcccagaaa aaggggacg
//
LOCUS       DASUCJ010001407         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_534777, whole genome shotgun sequence.
ACCESSION   DASUCJ010001407 DASUCJ010000000
VERSION     DASUCJ010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534777"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFU90_16355"
     CDS             complement(<1..978)
                     /locus_tag="VFU90_16355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5481421.1"
                     /translation="MAEPHTGSAMARETAEIPAAAERLLARTDVFAAIVERIERAKPR
                     IIVFCGRGSSGHVGVYLRYIFEVRLGLLASAAAPSVVTAYQRPAELRDALFIVVSQSG
                     RSPDLVNATQVARKFGALTLAIVNDENSPAAAASELVLPIGAGTEHAVAATKTVALSM
                     IAGAQLVATLARDDDLNDGLQNLPLRLSDALACDWSDWADSAAGAAATFVVGRGYALG
                     CVREIALKVAEILRVPALGYSAAELRHGPRASITPATPVLVLRQNDQAAATVDELVRD
                     LNDAGERVFTAGGAAGTLPWIGDGHPICDPVLMLIPAYRAIEAAARRRGF"
BASE COUNT          194 a          387 c          368 g          181 t
ORIGIN      
        1 gaaacctcgc cgacgcgccg ccgcctcgat ggcgcgatag gccggtatga gcatgaggac
       61 tgggtcgcaa atcgggtgac cgtcgccgat ccagggcagc gtccccgccg cgcctccggc
      121 ggtaaacacc ctctcacccg cgtcgttcag atcacggacg agctcgtcga cggtggcggc
      181 ggcttggtcg ttctgtcgca gcaccagcac cggcgttgcc ggcgtgatcg atgcgcgcgg
      241 cccgtgccgt agctcggccg cgctgtaacc gagagccggg acccgcaaga tttcggccac
      301 tttgagcgcg atctcgcgga cacaaccgag cgcgtatcct cgtcccacga caaaggtcgc
      361 cgccgcgccc gccgcactgt cggcccaatc cgaccagtcg cacgcgagcg cgtcggacaa
      421 tcggagcggc aaattttgga ggccgtcgtt taaatcgtcg tcgcgtgcca gcgtcgccac
      481 cagctgcgcc cccgcgatca tcgacagtgc gacggtcttg gtcgccgcaa cggcgtgttc
      541 ggtccccgcg ccgatcggca ggaccagttc agacgcggcg gctgcgggtg agttttcgtc
      601 attaacgatg gcaagcgtca gcgcgccgaa cttgcgcgca acttgcgtgg cgttgacgag
      661 atcgggacta cgcccggact gcgacaccac gatgaacagg gcatcgcgca attccgcagg
      721 ccgctggtag gcagtaacga cggaaggtgc ggcggcggat gcgagcaggc caaggcgcac
      781 ttcgaaaatg tagcgcagat aaacgccgac atgtcccgaa ctgccacgcc cgcagaaaac
      841 gatgatgcgc ggcttggccc gctcgatccg ttcgacgatc gccgcaaata catcggtacg
      901 cgctagaaga cgctcggccg ccgccgggat ttcggcggtc tcgcgtgcca tcgcacttcc
      961 ggtatgcggt tcagccatcg tcgccggctg ccgcattgat ggcggagcgt agattgcccc
     1021 cgtggcgctg cagcagctgc gcggcttcgc cgcggcccag tcccaagccg agcaggacgg
     1081 cagtcttcac gtccccgtct gcctgttcga gatagcgcgc cgcgtcgcct
//
LOCUS       DASUCJ010001408         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_626300, whole genome shotgun sequence.
ACCESSION   DASUCJ010001408 DASUCJ010000000
VERSION     DASUCJ010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626300"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFU90_16360"
     CDS             complement(<1..869)
                     /locus_tag="VFU90_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481422.1"
                     /translation="MKSSRSAPLLPSLDHVATLSDDTGIVQHAVESVPNRSSGYCTDD
                     IARAFIVALMRLQAAPRDETALRLASTYLSFLHDAQLPDGRFHNFMSYARDWLDDVGT
                     QDSAGRAMWALGYGMRFAPAQDWRRLCRAMFDRSLGAIDWFEYLHPRAYAMLGLAHAY
                     ETLRDTPYAAAARYLGDALTRAFEAQRGDGWDWFDVAMTYDNARLPEALIRIGSVLAD
                     SRYAGAGAASLAFYEQVTVEDGTFVPIGNRGWYERGGKRAIYAQQPLEASALIDAELA
                     AFDATGDTRRLAVA"
     gene            complement(866..2038)
                     /locus_tag="VFU90_16365"
     CDS             complement(866..2038)
                     /locus_tag="VFU90_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5481423.1"
                     /translation="MLPPRALFLGSYPPRECGIATFTKDVVDSYNRAFGISSQVVAID
                     EPGGEMRRYGPEVVARLAEQDRYGYAAVADFINRHPADLLNVQHEYGLFGGERGEWLV
                     DLLRALQKPVVVTLHTVLPEPDETMLRVTRELCASAARVVSLSETGRSLLEGVYGVDP
                     ETLRVIHHGVPDVPFQNTDAAKASFGIGQRTVVSTFGLISRGKGLEYAIEAMRDVVSR
                     HPETLYLILGATHPVVRRHEGESYRESLLAKVREYGLQYNVQLVDKYLDFDELVGYLA
                     ATDIYLTPYLNPAQIVSGTLAYAVGCGKAIISTPYLYAQELLAHSRGFLCEFRDAPSI
                     ARRLNMLLDDPTLRRATERRAYRFGRQMTWPHVSVEYGNLFLELAPPGSLELVSSA"
     gene            complement(2122..>2320)
                     /locus_tag="VFU90_16370"
     CDS             complement(2122..>2320)
                     /locus_tag="VFU90_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481424.1"
                     /translation="LWERVSVGPGAFIEEAIIASGATIGPKAQINKGSVIGHDVTIEP
                     GQVLEAGSRVGSPAKAASPSG"
BASE COUNT          398 a          772 c          777 g          373 t
ORIGIN      
        1 gcgacggcga gacggcgcgt atcgccggtt gcgtcgaacg cggcgagctc ggcgtcgatc
       61 agcgcgctcg cctccagcgg ctgctgcgcg tagatcgcac gctttccccc acgttcgtac
      121 cagccccggt tgccgatagg gacgaacgtc ccatcttcga ccgtgacctg ctcgtagaat
      181 gccagcgacg ccgcaccggc gccggcataa cggctgtcgg ccagcacgga cccaattcgg
      241 atcagcgcct cgggcagccg cgcgttgtcg taggtcatgg ccacgtcgaa ccaatcccag
      301 ccgtcgccgc gctgcgcctc gaaggcgcgc gtcagcgcgt cgccgagata acgggccgcg
      361 gcggcgtagg gtgtgtcgcg caacgtttcg taggcgtggg ccaggcccag catggcgtac
      421 gcgcggggat gcaggtactc gaaccagtcg atcgcgccca ggctccggtc gaacatcgcg
      481 cggcacaggc ggcgccagtc ctgcgccggc gcgaagcgca tcccgtagcc cagcgcccac
      541 atcgcccggc cggcgctgtc ctgggtaccg acgtcgtcga gccagtcgcg cgcgtagctc
      601 ataaagttgt ggaagcggcc gtccggaagc tgcgcgtcgt gcaggaacga gaggtacgtc
      661 gacgccagcc gcagggcggt ttcgtcgcgc ggcgccgcct gcaggcgcat cagcgccacg
      721 atgaaagcgc gcgcgatgtc gtcggtacag tagccgctcg accggttcgg gacggactcg
      781 accgcgtgct gaacgatgcc ggtatcgtcg gagagcgtcg ccacgtgatc cagcgatgga
      841 agcagcgggg cgctccgcga gcttttcatg ccgacgagac caactccagc gagcccggcg
      901 gcgccagctc gaggaacagg ttgccatatt cgacggagac gtgcggccaa gtcatctgcc
      961 gcccgaagcg atacgcgcgg cgttcggtcg cgcggcgcag cgtcggatcg tcgagcagca
     1021 tgttcagccg cctggcgatc gacggcgcgt cgcggaactc gcagaggaag ccgcgactgt
     1081 gcgcgagcag ctcctgcgcg tacaggtagg gagtcgagat gatcgcctta ccgcagccga
     1141 ccgcgtaggc gagcgttccg ctgacgatct gtgccggatt caagtacggc gtgaggtaga
     1201 tgtcggttgc ggcgaggtag ccgaccagct cgtcgaaatc gagatacttg tcgacgagct
     1261 gcacgttgta ttgcaggccg tactcgcgca ccttggcgag cagcgattcg cggtacgact
     1321 cgccttcgtg gcggcgcacg accggatggg ttgcgccgag gatcagatac agcgtttcgg
     1381 gatgacggct gacgacgtcg cgcatcgctt cgatcgcgta ctccaatccc ttgccgcggc
     1441 tgatcagccc gaaggtcgaa acgaccgtcc gctgtccgat gccgaacgac gctttggcgg
     1501 catcggtgtt ttggaatggg acgtcgggaa cgccgtgatg gatcacgcga agggtctcgg
     1561 gatcgacgcc gtagacgcct tccagcaggc tgcgtcccgt ttccgaaagc gacacgaccc
     1621 gggccgcgct ggcgcacagc tcgcgcgtca cgcgcagcat cgtctcgtcg ggctcgggca
     1681 gcaccgtatg caaggtgacg acgaccggct tttgcagcgc gcgcagcaaa tcgaccagcc
     1741 actcgccgcg ttcgccgccg aacaggccgt attcgtgctg aacgttcagc agatcggccg
     1801 gatggcggtt gatgaaatcg gcgaccgcgg cgtacccgta gcggtcctgc tcggcgaggc
     1861 gcgccaccac ctcggggccg tagcgccgca tctcgccgcc cggctcgtcg atcgccacga
     1921 cctggctcga aatgccgaat gcccggttat acgaatcgac gacgtctttg gtaaacgtcg
     1981 cgatcccgca ttcccgaggc ggatacgagc ctaagaacaa cgcgcgcggc ggcaacataa
     2041 cgatgtgcaa acccctccac tactgtggac taagcgtacc cgccgttgtc tgaggcataa
     2101 acctgcccgg cgggagaggg gtcaacccga aggagacgcg gccttggccg gcgacccaac
     2161 tcggctgcct gcttccaaca cctgccccgg ctcgatggtt acgtcgtgcc cgatgacgct
     2221 gcctttattg atctgcgcct tgggcccgat ggtggccccg ctcgcgatga tcgcctcttc
     2281 aatgaaggcg cccgggccga cgctgacccg ttcccacagg
//
LOCUS       DASUCJ010001409         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1008569, whole genome shotgun sequence.
ACCESSION   DASUCJ010001409 DASUCJ010000000
VERSION     DASUCJ010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008569"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFU90_16375"
     CDS             <1..620
                     /locus_tag="VFU90_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5481425.1"
                     /translation="IDYRSSSLKINVALSDVPNFTALPGAKPGPQHRGTIHVAPSLDY
                     IERAYDDSKYGRPSSSPVLECTIPSVVDPTVAPPGKHLMSMFVQYAPNKLCDASWDDI
                     KEQFADRCLDVLAEYAPNIKNAVIARQVLSPLDLERRFGLTGGNIFQGAMTFPQLFFL
                     RPVAGYADYRTPVDNLYLCGAATHPGGGVMGACGYNAAREMLRDA"
     gene            complement(624..>1095)
                     /locus_tag="VFU90_16380"
     CDS             complement(624..>1095)
                     /locus_tag="VFU90_16380"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5481426.1"
                     /translation="PANSGENDLYDGPFLGGFMVITQQGGSMSMSGRACGLPVGDLPS
                     EDLQRVYYYSIFPNMLLSLHPDYAMAHTLWPQAADQTRITCEWLFNPASFADASFDAN
                     DAIAFWDMTNAQDWHVCELSQLGVSSSAYTPGPYSARESLSVAFDNEVRRSVAD"
BASE COUNT          192 a          380 c          343 g          180 t
ORIGIN      
        1 agatcgacta ccgcagctcg tcgctcaaga tcaacgtcgc gttgagcgac gtgccgaact
       61 tcaccgcgct ccccggcgcg aagccaggcc cgcagcatcg cggcacgatt cacgtcgcgc
      121 cgtcgctcga ctacatcgag cgcgcctatg acgactcgaa gtacgggcgc ccgtcgtcgt
      181 cgcccgtgct cgagtgcacc atcccgtcgg tggtcgaccc cacggtcgcg ccgccaggga
      241 agcacctcat gtcgatgttc gtccagtacg cgcccaacaa actgtgcgac gcgagctggg
      301 acgacatcaa agagcagttc gccgatcgct gcctcgacgt gctcgccgag tatgcaccga
      361 acatcaagaa tgccgtcatc gcgcggcagg tgctctcgcc gctcgatctg gagcgccgct
      421 tcggcctcac cggcggcaac atcttccagg gcgcgatgac gttcccgcag ctgttcttcc
      481 tacgccccgt ggccgggtac gccgactacc gcacgccggt ggacaatctg tatctctgcg
      541 gcgcggcgac gcaccccggc ggcggcgtca tgggcgcgtg cggctacaac gccgcgcgcg
      601 agatgctgcg cgatgcctaa cggttagtcg gcgacgctcc gccgaacctc gttgtcgaac
      661 gccacggaca gactctcgcg cgccgagtag gggccgggcg tgtatgccga cgacgacacg
      721 cccaactggc tcagctcgca cacgtgccag tcctgcgcgt tggtcatgtc ccagaacgcg
      781 atcgcatcgt tcgcgtcgaa cgacgcgtcg gcaaaggaag cgggattgaa cagccactcg
      841 cacgtgatgc gcgtctggtc cgctgcctgc ggccacagcg tgtgcgccat cgcgtagtcg
      901 gggtggaggc tgagcagcat gttcgggaaa atcgagtagt agtacacgcg ctgcaggtcc
      961 tcactgggaa ggtcgcccac cggaagacca cacgcgcggc cgctcatgct catgctaccg
     1021 ccctgctgcg tgatcaccat gaaaccgccg aggaacgggc cgtcgtacag atcgttctcg
     1081 ccgctgttgg caggt
//
LOCUS       DASUCJ010001410         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_534802, whole genome shotgun sequence.
ACCESSION   DASUCJ010001410 DASUCJ010000000
VERSION     DASUCJ010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534802"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFU90_16385"
     CDS             <1..808
                     /locus_tag="VFU90_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5481427.1"
                     /translation="LYDVSFVIPAGHNIAVVGPSGSGKSTLARLLYRFYDVDAGRITI
                     DGQDLRDVQQATLRAAIGIVPQDTVLFNDTIEYNIGYGNPDASHAAIVEAARLAQIHD
                     FVSRLPDGYRTAVGERGLKLSGGEKQRVAIARAILKNPAILIFDEATSALDSRAEKAI
                     QSELQVIARNRTTLTIAHRLSTIVDSDLILVLEHGRIVERGTHVALLAAGGVYARMWR
                     LQQDERRSASDEDNSPPPERVHAGSDRAADPAEPSAAAAAAAAAGARGAL"
     gene            888..>1623
                     /locus_tag="VFU90_16390"
     CDS             888..>1623
                     /locus_tag="VFU90_16390"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5481428.1"
                     /translation="MNRLLLIVFATIVSAPVWASPSAPDPARLKLKSANAVVLDADTD
                     RQIYAKAANEVTPIASLTKLMTAMVVLDAKQPMDDPITIDTDDIDTLKNSGSRLRMGA
                     TLSRREMLRLALMASENRAASALARYYPGGKAAAVEAMNAKATLLGLAQTHYADPTGL
                     SPDNVSTAHDLAKLVQAAAEYPLIREFTTTPKLAVDIEPTGRTVGFVNTNALVRGQSW
                     DILMQKTGYIREAGRCVVMLVNIASRP"
BASE COUNT          292 a          535 c          540 g          256 t
ORIGIN      
        1 cctttacgac gtcagcttcg tgattcccgc cggccacaac attgccgtcg tcgggccgtc
       61 gggatcgggc aagtcgacgc tcgcgcgact tctctaccgg ttctacgacg tcgacgcggg
      121 gcgcatcacg atcgacgggc aggatctgcg cgacgtgcag caggcgacgc tccgcgcggc
      181 gatcgggatc gtcccgcagg acaccgtcct cttcaacgac acgatcgagt acaacatcgg
      241 ctacggcaac ccggacgcat cccacgcggc gatcgtcgag gccgcgcgcc ttgcgcagat
      301 tcacgacttc gtgtcgcggc ttcccgacgg ctaccgcacc gcggtgggcg agcgcgggct
      361 gaagctctcg ggcggggaga agcagcgcgt cgccatcgcg cgggcgatcc tcaagaaccc
      421 cgcgatcctg atcttcgacg aagcgacgtc ggcgctcgac tcgcgcgccg agaaggcaat
      481 tcagtccgag ttgcaggtga tcgcgaggaa ccgcacgacg ctgacgatcg cgcaccgact
      541 ctcgacgatc gtcgactccg acctcatcct cgtcctcgag cacgggcgga tcgtcgagcg
      601 cggcacacat gttgcgttgc tcgctgccgg gggcgtctat gcccgtatgt ggaggttgca
      661 gcaggatgag cgccgatcgg cgtcggatga ggacaattcg ccgccgccgg agcgggtcca
      721 cgccggcagc gaccgggccg cagatccggc cgagccgagc gcggccgcgg cagcggcggc
      781 ggcagccggg gcacggggtg cgttgtaggt cattgattcg taaggaaggt tgacgtcacg
      841 tccggatccg tgcgataagc cgcgtggaaa tgttgagagt ttcgctgatg aaccgcctgc
      901 tactcattgt ttttgcgacc atagtcagcg cacccgtctg ggcgagcccg agcgcgcccg
      961 atccggcccg cctgaagctc aagtccgcca atgccgtcgt cctcgacgct gacacggatc
     1021 gtcagattta cgccaaggcc gccaacgaag tgacgccgat cgcgtcgctc accaagctga
     1081 tgacggcaat ggtggtgctc gatgcaaagc agccgatgga cgatccgatc acgatcgaca
     1141 ccgacgacat cgacacgctg aagaacagcg gctcgcgcct gcggatgggc gcgacgctgt
     1201 cgcgccgcga gatgctccgg cttgcactga tggcgtccga aaatcgtgcc gcgtcggcgc
     1261 tcgcgcgcta ctaccccggc ggcaaggccg ccgcggtcga ggcgatgaac gcgaaggcaa
     1321 cgctgcttgg ccttgcacag acgcattacg ccgacccgac cggtctatcg ccggataacg
     1381 tgtcgacggc gcacgatctc gcgaagctgg tccaggcggc ggccgagtat ccgctgatcc
     1441 gcgagttcac gaccacgccg aagctcgcgg tggacatcga acccaccgga aggaccgttg
     1501 ggttcgtcaa caccaatgca ctggtgcgcg ggcagtcgtg ggacatcctg atgcagaaga
     1561 ccggctacat ccgcgaggcc gggcgctgtg tcgtgatgct ggtgaacatc gcgagccggc
     1621 cga
//
LOCUS       DASUCJ010001411         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_595956, whole genome shotgun sequence.
ACCESSION   DASUCJ010001411 DASUCJ010000000
VERSION     DASUCJ010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595956"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFU90_16395"
     CDS             complement(<1..278)
                     /locus_tag="VFU90_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5481429.1"
                     /translation="MDADAIVVGAGAAGLAAARHLARASKRVIVVEARERAGGRVWPH
                     SAIEGAAPGELGAEFIHGQAELTYRLLREAGLTWDGVEGERWYAMPDGA"
     gene            350..949
                     /locus_tag="VFU90_16400"
     CDS             350..949
                     /locus_tag="VFU90_16400"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEU5481430.1"
                     /translation="MTGAPVLEFWFDFASTYSYVAASRIETLCRDAAVALRWKPFMLG
                     PIFEMQGWRTSPFVVNPLRGGYMWRDLERLTAKYGLPWKKPSAFPRTAALAAKVAAAH
                     DGEPWLGDFVRTAFAAAFGRDADLNDRRVVASLLAAAGTDSENALRIATETPRKSELR
                     NNTERAMSLGIFGAPNCLVGDELFWGEETLEDAVAWARR"
     gene            complement(973..1317)
                     /locus_tag="VFU90_16405"
     CDS             complement(973..1317)
                     /locus_tag="VFU90_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5481431.1"
                     /translation="MNAVSLIIYPASDVPKATKFFATLLGTEPYAESQAYVGFKTGDM
                     EIGLVPQAAQRGTGALAYVTVSDINAALETLLAAGAEKVQDVTDVAYGLLVASVKDPD
                     GTPIGLRQFPAR"
     gene            complement(1398..2246)
                     /locus_tag="VFU90_16410"
     CDS             complement(1398..2246)
                     /locus_tag="VFU90_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5481432.1"
                     /translation="MTDWLADRFEENRSHLRNVAYRMLGSLDDADDAVQEAWLRLSSR
                     ENGGIENLGGWLTTVVSRICVDMLRARRSKREEPLERTIPDFIVSRKESPEDEVLTAD
                     SVGLALLVVLDSLTPSERLAFVLHDMFAMPFEEIASIVGRSPVAARQLASRARRRVQG
                     ATPPIAGFAQRRSVVDAFIAAAREGDFDALVAVLDPDVLLRADREPAAKGIRGAQAVA
                     SQAAAFSKVVQTMEPVIVNGSPGILSWLPNGEPLSVMGFVVAGNRITEMYVISQPDRL
                     RQLLGL"
     gene            complement(2256..2735)
                     /locus_tag="VFU90_16415"
     CDS             complement(2256..2735)
                     /locus_tag="VFU90_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5481433.1"
                     /translation="MTTQTIELKPRMKHPIAIFPEAMQALQVLGKTAHNRAVPLRTLM
                     LVELRASQINGCSVCVDMHSRDLKKHGDSDERIFGVAAWRENPAFDDAERAALALAES
                     LTRISDRPDPVSDEVWAEAARHYDEAGLATLLIAIANINVWNRLNVGVRQIVGPWSA"
     gene            complement(2866..2941)
                     /locus_tag="VFU90_16420"
     tRNA            complement(2866..2941)
                     /locus_tag="VFU90_16420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2906..2908),aa:Asn,seq:gtt)
BASE COUNT          505 a         1043 c          993 g          561 t
ORIGIN      
        1 gctccgtccg gcatcgcgta ccagcgttcg ccctcgacgc cgtcccacgt caaaccggct
       61 tcgcgcagca gccggtacgt gagttcggct tgcccgtgga tgaactcggc gccgagttct
      121 cccggcgcgg ccccctcgat cgccgagtgc ggccacaccc ggccgccggc gcgctcgcgc
      181 gcttcgacga cgatgacgcg cttggacgcg cgtgcgagat gccgtgccgc cgccaaaccc
      241 gccgctcccg cacccacgac gattgcgtcc gcgtccacgc tagccgcgca tttccatcgc
      301 aagcgtggtt tgagaggtcg cgcgcggcgc cctgcgaaaa cgcaaccgca tgaccggcgc
      361 tcccgtgctc gagttttggt tcgacttcgc gagcacgtat tcgtacgtcg ccgcctcgcg
      421 catcgaaacg ctctgtcgcg atgccgccgt ggcgcttcgc tggaaaccgt tcatgctggg
      481 gccgatcttc gaaatgcagg gctggcggac gtccccgttc gtcgtgaacc cgctgcgcgg
      541 cggctacatg tggcgcgacc tggagcgttt gaccgcgaaa tacggcctgc cctggaaaaa
      601 gccgagcgcg tttccgcgca ccgccgcgct cgcggccaag gttgccgccg cgcacgacgg
      661 cgagccctgg ctaggcgact tcgtccgtac ggccttcgcc gccgcgtttg gtcgcgacgc
      721 ggatctcaac gatcgccgcg tcgtcgcttc gctcctcgcg gcggccggca ccgattccga
      781 gaacgccctc cgcatcgcca ccgagacgcc gcgcaaatcc gagctgcgga acaacacgga
      841 acgagcgatg tcgttgggga tcttcggcgc gcccaactgt ctggtcggcg acgaactgtt
      901 ctggggcgaa gaaacgctcg aagacgccgt cgcctgggcg cgccgctaaa acgaaatccc
      961 gcgagcctgg tgctaccgcg ccgggaactg acgcagtccg atcggcgttc cgtcggggtc
     1021 tttgacgctc gccaccagca acccgtaagc gacgtcggtg acgtcctgca ccttctcggc
     1081 gccggctgcg agcagcgtct ccagcgccgc gttgatgtcg ctgacggtca cgtacgcgag
     1141 cgctcccgtg ccgcgctgcg ccgcctgcgg gacgagcccg atctccatgt cgccggtttt
     1201 gaatccgacg taagcttggc tctcggcgta cggctcggtg ccgagcagcg tcgcgaagaa
     1261 cttcgttgcc ttcggcacgt ccgaggcggg gtagatgatg aggctaaccg cgttcatgtt
     1321 gcgctcctag tgagatgagt tttgcagggt gacgaattcc ggcggcggga tgtgacgcgc
     1381 gccgatcgcc ggttggctta aagtccgagg agctggcgca gccggtccgg ttgtgagatc
     1441 acgtacatct cggtaatccg gttccccgcg accacgaacc ccatcaccga gagcggttcg
     1501 ccgttgggaa gccacgacag gattccgggc gatccgttga cgatgaccgg ctccatcgtt
     1561 tgcactacct tggagaaggc tgccgcctgc gaagcaaccg cctgcgcgcc gcgaatgccc
     1621 ttggcggccg gttcgcgatc ggcgcgcagc agaacgtcgg gatcgagcac cgccaccagc
     1681 gcgtcgaagt cgccttcgcg tgctgccgcg ataaacgcgt cgaccaccga gcgccgttgc
     1741 gcgaagccgg cgatcggcgg cgtcgcgccc tgcacgcgcc gccgcgcgcg gctggccagc
     1801 tgacgggccg cgaccgggga ccggccgacg atcgatgcga tctcctcgaa cggcattgca
     1861 aacatgtcgt gaaggacgaa cgccagccgt tccgaggggg tcaggctatc gaggacgacc
     1921 agcagcgcca aacctaccga gtcggccgtc aggacctcgt cttcggggct ttctttgcgg
     1981 ctcacgatga agtccgggat ggtgcgctcg agcggttcct cgcgcttgga gcgccgggcg
     2041 cgcagcatgt cgacgcaaat ccgcgacacg accgtcgtca gccagccccc gagattctcg
     2101 atgccgccgt tctcgcgcga gctcagccgc agccacgcct cctgcactgc atcgtcggcg
     2161 tcgtcgagcg agccgagcat ccggtaggca acgtttcgca aatgggaccg gttttcttca
     2221 aaccggtccg ccagccaatc cgtcatcgcg ccgaactacg cgctccaggg tccgacgatt
     2281 tgtcgcacgc cgacgttgag ccggttccag acgttgatgt tcgcgatggc gatgagcagc
     2341 gtggcgagcc cggcttcgtc gtagtgacgc gctgcttccg cccagacttc gtcggaaacg
     2401 ggatccggcc ggtcgctgat gcgcgtcagc gattcggcga gcgcgagcgc ggcccgctcg
     2461 gcatcgtcga acgcggggtt ttcccgccac gcggcgacgc cgaagattcg ttcgtcgctg
     2521 tcgccgtgtt tcttgagatc gcgcgagtgc atgtcgacgc aaacgctgca gccgttgatt
     2581 tggctcgcgc gcagttcgac gagcatcagc gtgcgcagcg gcacggcgcg gttgtgtgcg
     2641 gtcttgccta ggacctgcag cgcctgcatt gcttcgggaa atatcgcaat cgggtgtttc
     2701 atgcgcggtt tgagttcgat cgtctgggtg gtcatgcgct ctcctagtct gcctggtctt
     2761 gcgttgtacc tataccactc tgacgggaga ccgccgcgcg atgtgacgtg aaacgatgta
     2821 acgcggtgaa ggataaggcc cgcgcgaagc gggccttatc ttgattggct ccggatgctg
     2881 gactcgaacc agcgacccgc tgattaacag tcagctgctc taccaactga gctaatccgg
     2941 aatgctgcgt gggctacggc gattttaccg ccgcccgagg ttccacgccc gccccgccgg
     3001 ccctttaacc tatctcttaa tatattagtt gacagaaccg gccctagcga gtaggctaag
     3061 gctaacaact aaaactttaa gagccaggag accccaaatg ct
//
LOCUS       DASUCJ010001412         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_794457, whole genome shotgun sequence.
ACCESSION   DASUCJ010001412 DASUCJ010000000
VERSION     DASUCJ010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794457"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFU90_16425"
     CDS             complement(<1..298)
                     /locus_tag="VFU90_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU5481434.1"
                     /translation="MRSVWEFFAVAIVVTITPGPGTTTVLRVAARDGRRAATQAILGN
                     SAGVLTWGALSAVGVSSLILASQRRTTSSASGVRLYSSRSDSAHCLDATPRPSSG"
BASE COUNT          188 a          359 c          363 g          155 t
ORIGIN      
        1 acccgctgga tggcctcggc gtggcgtcca agcagtgagc ggagtccgag cgcgacgagt
       61 acagccgcac ccccgatgcg gaggacgtcg tacgccgttg tgaggcgagg atcagcgacg
      121 agactcccac ggccgacagc gcaccccagg tgaggacacc ggcgctgttg ccgaggattg
      181 cttgggtcgc tgcgcggcga ccgtcgcgtg ccgccacgcg caagactgtc gtcgtacccg
      241 gaccaggcgt gatcgtcact acgatcgcga cggcaaagaa ctcccaaacc gagcgcatgc
      301 cctgaagata ttgcgcccgc gggcgccccc gcgcagttct gcttcggcgg aatgcgagcg
      361 cctgaccgac ccgatcgcgg cctctgaaac gctcgttcgt gatcaatcta gcagcgccgc
      421 cgactcgatc ggcgagacgc ccggtttcgc gacgaagcgc aagcccgaac gcgcccgatg
      481 cttgcagcaa gcgcgccgaa tcagcaagca cccgcgctgc tgctccccta ggaactcggg
      541 cgcgtcgatc gtccccgctc agagttgcga gttggacgag gaccgggcgt gattcgccgt
      601 tcccgtcgac gcaacgtcga cagcgatcct cgtcgtccaa tccggatgcc agcgaggtca
      661 tgcccgcgat cgagctcgtc acgaatcctc ggccggagcc gaacgtccgg tcgagccgta
      721 gtccgcgcgc gctcggtgcc gcgcccgatg gcacgccgac agcgacggcg gagagttcgg
      781 cgaccatcaa ccaggccggc cgtcgggtca gggcgtgagg aacgcagcgg cccagactac
      841 gttccgagac cacagcgccg gtaggcgcgt ctgcgatact cgctgtccgc gcacgatcga
      901 taacggaggg atggatgatg cgtaaggggg ccgggcgctt gatgcgcaca gtggtcttag
      961 cggcagctgt cgtctgctcg atgacgttca gcatggcgag ctcaccgacg gcgtccgcgg
     1021 cgagcgtgcg cacgaagatc gacgcgctga ttcggggcgc gatcg
//
LOCUS       DASUCJ010001413         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_198507, whole genome shotgun sequence.
ACCESSION   DASUCJ010001413 DASUCJ010000000
VERSION     DASUCJ010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198507"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFU90_16430"
     CDS             <1..513
                     /locus_tag="VFU90_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5481435.1"
                     /translation="FKRLHGQSGGAGGTDNEFAARSFENVGAWILGRNMFGPVRGPWG
                     DESWKGWWGENPPYHTPVFVITHYPRPSVAMQGGTMFHFVTDGIETALERAREAAGSK
                     DVRIGGGVSTIRQYLLAGCIDEIHLAFSPVLLGEGEHLFAGLDLPKRGFGSVRVTPGE
                     RATHVLLTKI"
     gene            complement(525..>1174)
                     /locus_tag="VFU90_16435"
     CDS             complement(525..>1174)
                     /locus_tag="VFU90_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5481436.1"
                     /translation="FGVQILMSERTIVASGSDLLVALANAAVPRPWSDIAVIVVVVSA
                     IGSLETTLVSGSRLVYSMGRDRVLDERFGDLNARYLTPWHATIAIGLVSMALFALAAT
                     SSSVNAILSDSINAIGVEVAIFYGLSAVACASYYAAADRRDVLLVWLRRGWPIAAALF
                     VFGVALGQVFEAGLRANVTVFGLLLVGVIPMLYYRRRYASDYYREPLERAPAETA"
BASE COUNT          243 a          352 c          392 g          187 t
ORIGIN      
        1 ttcaagaggc tgcacgggca aagcggagga gccggtggga cggataacga gttcgctgcc
       61 cggtcattcg aaaacgtcgg tgcatggatt ttaggccgga acatgttcgg gccggttcgc
      121 ggaccatggg gagacgagtc gtggaagggt tggtggggcg agaatccgcc ctatcacacg
      181 ccggtcttcg tcatcacgca ctatccacgg ccttccgtcg cgatgcaggg cggcacgatg
      241 ttccatttcg ttaccgatgg aatcgagacg gccttagagc gagcaagaga agcggctggg
      301 agcaaagacg ttcgaatagg cggaggtgtt tccacgatac ggcaatacct gctggccggc
      361 tgcatcgacg aaatccatct tgcgttttcg cccgtactgc tcggcgaagg cgagcaccta
      421 ttcgcggggc tggatcttcc caaacgaggc ttcggctcgg ttcgggtgac tccgggcgaa
      481 cgcgcgacgc acgtcctgct gacgaaaatc taaccggcac cgcgtcaggc ggtttcggcg
      541 ggggcgcgtt cgagcggctc gcgatagtaa tcgctcgcgt accggcggcg gtagtagagc
      601 attggaatga cgccaacgag gagcaggccg aagacggtta cgttcgcgcg gagtcccgcc
      661 tcgaacacct ggccgagggc caccccgaag acgaagagtg ccgcggcgat cggccagccc
      721 cgccgcagcc aaaccagcag cacgtcgcga cgatcggcgg cggcgtaata cgacgcgcaa
      781 gcgactgccg agagaccgta aaaaatcgcg acctcgacgc cgattgcgtt gatcgagtcg
      841 ctcaatatcg cgttgacgct ggaactcgtg gccgccagcg cgaacaacgc catcgagacg
      901 aggccgatgg cgatcgtcgc atgccacggc gtcagatagc gcgcgttgag gtcgccgaag
      961 cgctcgtcga gcacgcggtc gcggcccatc gagtacacga gccggcttcc cgatacgagc
     1021 gtcgtctcca gcgatccgat cgcgctgacg acgacgacga taacggcgat gtcgctccac
     1081 ggccgcggga cggcggcatt ggcaagcgcg accaggagat ccgagccgga agctacgatc
     1141 gtccgctcgg acattaaaat ctgtacgccg aacg
//
LOCUS       DASUCJ010001414         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_243996, whole genome shotgun sequence.
ACCESSION   DASUCJ010001414 DASUCJ010000000
VERSION     DASUCJ010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243996"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1800
                     /locus_tag="VFU90_16440"
     CDS             <1..>1800
                     /locus_tag="VFU90_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5481437.1"
                     /translation="HGITDVVATLHYLADEIENYFGDGSEMGVSMAYVVEDTPLGTAG
                     AIKLAQPLLGSERFVIISGDALTDIDLTELVAVHERNGAAATIALQRVSNPLEFGVVV
                     TDERGRITRFLEKPSWGEVFSDTINTGIYVLEPEIFEYMESGKNYDFSRDLFPRMLRD
                     GKPLFGHVAAGYWSDIGNLQQYLQANYDALSGAVRVEIPGTEIRPGVWAEADSRISPD
                     AHVHGPVSIGRNVTIEPGAVVEELTAVGASSIVAANARLHRSITWEDVYVGESATLTG
                     CTLADHVIVKDRVTIMEGAVIGRRSTLGSGATVPSNIKLWPDKAVAAGSIVSMSLIYG
                     IKWPGSLFGADGVSGLANIEITPEFTLKLGQALGSVLQPGQTVTTSRDAHPASRLTNR
                     CIIAGLLSVGVNVQDLREVPTPVSRHAVRASGDAGVHTGISPADPDQFLIEFFDHHGV
                     NIDKTTERKIENIFFREDFRRTPMESVGLLSFPERTVEGYSNDFLTALNPRSMSSANM
                     RVIIDYAFGNASLILPRILSNLGIEMIALNAFFDNSKVLTFARNRGRHLEQLANVTTS
                     LDATLGILLDQHGEALALVDDRGRAIEKSRLLA"
BASE COUNT          326 a          625 c          547 g          302 t
ORIGIN      
        1 gccacggcat caccgacgtc gtcgcaaccc tgcactatct ggccgacgag atcgagaact
       61 acttcgggga cggctccgag atgggcgtct cgatggccta cgtcgtcgag gacacgccgc
      121 tgggcacggc cggtgcgatc aagctcgcac agccgctgct cggcagcgag cgattcgtca
      181 tcatatcggg cgacgcgctc accgacatcg acctcaccga gctggttgcc gtgcacgagc
      241 gcaacggtgc tgccgcgaca atcgcgctgc agcgcgtttc caacccgctc gagttcggcg
      301 tggtcgtcac cgacgaacgc ggccggatca cgcggttctt ggaaaaaccg tcgtggggcg
      361 aggtgttttc cgacaccatc aacaccggca tctacgtgct cgagcccgag attttcgagt
      421 acatggaatc cggcaagaac tacgatttct cgcgcgacct cttcccgcgc atgctgcgcg
      481 acggcaagcc gctgttcgga cacgtcgcgg ccggctactg gagcgacatc ggcaacctgc
      541 agcagtacct gcaggccaac tacgacgcgc tctccggcgc ggtgcgcgtc gagatccccg
      601 gaaccgaaat ccggccgggc gtgtgggcgg aggccgacag ccgcatttcg ccggacgcgc
      661 acgtccacgg tccggtctcg atcggacgca acgtgacgat cgaaccgggc gccgtcgtcg
      721 aggagctgac ggcagtcggc gcctcttcga tcgtcgccgc caacgcgcgc ctgcatcgca
      781 gcatcacgtg ggaagacgtc tacgtcggcg aaagcgccac gctgaccggc tgcaccctcg
      841 ccgatcacgt gatcgtcaaa gaccgcgtca ccatcatgga aggcgcggtg atcggacggc
      901 gcagcacgct gggcagcggc gcaaccgttc cgtcgaacat caagctctgg cccgacaaag
      961 cggtggcggc cgggtcgatc gtctcgatgt cgctgattta cggcatcaag tggcccggtt
     1021 cgctcttcgg cgccgacggc gtttcgggac tcgccaacat cgaaatcacg cccgagttca
     1081 ccctcaagct cggtcaagcg ctcggctcgg tgctgcagcc gggacagacg gtgacgacca
     1141 gccgcgacgc ccatccggcg tcccgattga ccaaccgctg catcatcgcg ggtttgctgt
     1201 cggtcggcgt caacgtccag gatctgcgcg aggtgccgac cccggtgtcg cgccacgcgg
     1261 tgcgcgcctc cggcgacgcc ggcgtgcaca ccggcatctc gccggccgat cccgaccagt
     1321 ttctgatcga attcttcgac catcacggcg tcaacatcga caagacgacc gagcggaaga
     1381 tcgagaacat cttcttccgc gaagattttc gccgcacccc gatggaatcg gtcggcttac
     1441 tgagcttccc cgagcgcacg gtcgagggct acagtaacga ttttctcacc gccctcaatc
     1501 cgcgctcgat gtcgtcggcc aacatgcggg tgatcatcga ctacgctttc ggcaacgcgt
     1561 cgctgattct gccgcgcatc ctcagcaact tgggaatcga gatgatcgcg ctcaacgcgt
     1621 tcttcgacaa ctcgaaggtg ctgacgtttg cccgcaaccg cggccgccat ctcgaacagc
     1681 tcgccaacgt cacgacctcg ctcgacgcca ccctcggcat cctgctcgat cagcacgggg
     1741 aagcgctcgc gctggtcgac gaccgcggcc gcgccatcga aaagagccgt ctgctggcgc
//
LOCUS       DASUCJ010001415         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_122265, whole genome shotgun sequence.
ACCESSION   DASUCJ010001415 DASUCJ010000000
VERSION     DASUCJ010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122265"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFU90_16445"
     CDS             <1..281
                     /locus_tag="VFU90_16445"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU5481438.1"
                     /translation="GFWRPGVVWFGEPLPPDAWERAAGAAARADVILVVGTSAVVYPA
                     AALATAYGDAYLVEINPETTAISQRVDLRLAGPAAERLPQLAAALGCG"
     gene            314..709
                     /locus_tag="VFU90_16450"
     CDS             314..709
                     /locus_tag="VFU90_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481439.1"
                     /translation="MDAVQLLKSKIPDFPGYADDLARQRSDELVRSYLGEAIADLQAR
                     LSPLDDAPARQIGDLLFRAGFTNQNVYRTYEDAARTRTDYDAMAAADARVVDLADRAP
                     SVDAASLETYLDGAREALDARDNAMDGAA"
     gene            complement(706..1512)
                     /locus_tag="VFU90_16455"
     CDS             complement(706..1512)
                     /locus_tag="VFU90_16455"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU5481440.1"
                     /translation="MKRRCVLVAAVVAVVVLAASAPSLADDKQLQNQKGSVSYQIPNG
                     KPVPLGVNATIGLADRDFAITGGSSLAQILLPDSSGVLVGSDTRVQLAFFNQTQIATA
                     NFVVYQGRVRFAVRHPHGAQANYTFKTATGSVGVRGTQGDIEYDSSGALRVNVYELCD
                     PNAPVEVTTKGGKTYSVAAGQTLLAQLVNGVVRTEVQSLTQQLINQFSPDFGVPTSWD
                     AAKGEVIGYGTSAVNSVTNGYGSEVASTITSVFGKKKTAPSPGPTPTTCS"
     gene            1588..>2529
                     /locus_tag="VFU90_16460"
     CDS             1588..>2529
                     /locus_tag="VFU90_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEU5481441.1"
                     /translation="MKASLRACVIVAAAALGLPGGASAAPPRLHLVPYPARADRLPCA
                     IPARALRTVPGGFDPAAATEIDERWKALGIGTLRVASAPAVRVRRDPSLAAQAYRLIV
                     DGGRATIESSDSDGAFYAAMTLAQLPVRSPEGGWSVPCVRIEDRPALRWRVLSDDVSR
                     GPLPTMRYFEERIRTIAAFKMNGYSPYMEHVFVAPSDPLPAPLDGITPDQLRTLTAYA
                     ARFHVALIPEQQTFAHMHGTLRVETYASAAELPHGFLLSPNVSLSQQYLARVIAAELA
                     AVGRPPFFHIGSDETATLGLGQTRDYVAQRGLSAAFAD"
BASE COUNT          373 a          877 c          834 g          445 t
ORIGIN      
        1 gcggattttg gcggcccggt gtcgtgtggt tcggggagcc cctgccgccg gacgcttggg
       61 agcgcgccgc cggcgccgcc gcgcgggccg acgtaattct ggtggtcggc accagtgcgg
      121 tggtctatcc ggccgccgcg ctcgcgaccg cgtacggcga cgcctatctc gtcgaaatca
      181 atcccgagac gaccgccatc agccagcgcg tcgatctgcg tcttgcgggc ccggccgcgg
      241 agcggctgcc gcagctcgcg gcggcgttgg gctgcggcta ggcgcgaacg ttcccggcgt
      301 cgtagcgata cgcatggacg cggtgcagct cttgaaaagc aaaattccgg atttccccgg
      361 ctacgccgac gatctcgcgc gccagcgatc ggacgagctg gtccgttcct atctcggcga
      421 agcgattgcc gatttgcagg cgcgcttgag cccgctcgac gacgcgccgg cgcggcaaat
      481 cggcgacttg ctgttccgcg ccggtttcac caaccagaac gtctatcgga cctacgagga
      541 cgccgcgcgg acgcgcaccg actacgacgc gatggccgct gccgacgcgc gcgtcgtcga
      601 cttggccgat cgcgctccgt cggtcgatgc cgcgagtctg gaaacctatc tcgacggcgc
      661 gcgcgaagcg ctcgacgcac gcgacaacgc gatggacggc gcggcttaac tgcaggtcgt
      721 cggagtcggg cccgggctcg gcgccgtctt cttttttccg aagaccgacg tgatcgtcga
      781 ggccacttcg ctgccgtaac cgttggtgac gctattgacg gcgctcgtgc cgtagccgat
      841 gacttccccc ttggcggcgt cccagctggt cggcacgccg aaatcgggcg aaaactggtt
      901 gatcagctgc tgcgtaagcg attgcacctc ggtgcgaacg accccgttga cgagttgagc
      961 caacagcgtt tgaccggccg ccacggagta cgtctttccg cctttggtgg tcacttcgac
     1021 cggcgcgttc gggtcgcaca actcgtagac gtttacgcgc agcgctccgc tcgagtcgta
     1081 ttcgatgtcg ccctgggttc cgcgcacgcc cacgcttccg gtggccgttt tgaacgtata
     1141 gttggcctgc gcgccgtgcg gatgccgcac cgcgaagcgt acccgcccct gatacacgac
     1201 gaagtttgcg gtagcgatct gcgtttgatt gaagaacgcc agctgcacgc gggtgtccga
     1261 gcctaccaaa acgcccgagc tgtcggggag caggatctgc gcgagcgacg agccgcccgt
     1321 gatggcaaaa tcgcggtcgg ccagcccgat ggtcgcgttg accccgagcg gcacgggttt
     1381 tccattggga atttgatacg agacgctgcc tttttgattt tgcagctgct tgtcgtcggc
     1441 cagcgacggt gcggaggcgg cgagcaccac gacggccacg accgcggcga cgaggacgca
     1501 acggcgtttc atgcccgcaa cccttacgcc ttcacgcggt tgagacctgg cgggaacgcc
     1561 gtcgtgcgac gccaaacgcg ctgcagcgtg aaggcttcgc ttcgggcctg cgtcatcgtt
     1621 gccgcggcgg cgctcgggct gcccggcggc gcatccgccg caccgccgcg ccttcacctg
     1681 gtgccctatc cggctcgcgc cgaccggctc ccgtgcgcga ttccggcacg cgctctgcgc
     1741 accgttccgg gcggcttcga tccggccgcc gctacggaga tcgacgagcg ctggaaggcg
     1801 ctcggcatcg gcacgctgcg cgtcgcaagc gccccggcgg tgcgcgtccg gcgcgatccc
     1861 tcgctcgccg cacaggccta tcgcctgatc gtcgacggcg gtcgcgcgac gatcgaaagt
     1921 tcggattcgg acggagcttt ctacgcggcg atgacgctcg cgcagctgcc ggtccgctcg
     1981 cccgaaggcg gctggtccgt cccgtgcgtc cgcatcgaag atcgcccggc gttgcgctgg
     2041 agagttctct cggacgacgt ctcccgcggg ccgctgccga cgatgcgcta cttcgaagaa
     2101 cgcatccgga cgatagccgc gttcaaaatg aacggctatt cgccgtacat ggagcacgtg
     2161 ttcgtcgccc cctccgatcc gttacccgcg ccgctcgacg gaattacgcc cgatcagctg
     2221 cgcacgctga cggcgtacgc ggcgcgcttc cacgtggcgc tgattcccga gcagcaaacg
     2281 ttcgcgcaca tgcacggcac gctgcgcgta gaaacctacg cttcggcggc ggagcttccc
     2341 cacggctttc tgctgtcgcc gaacgtttcg ctttcgcagc aatacctcgc ccgcgtgatc
     2401 gcggccgaac tggccgccgt cgggcgtccg ccgttctttc acatcggctc cgacgagacc
     2461 gcgacgctcg ggctggggca gacgcgcgac tacgtcgcgc aacgcggact atcggcggcc
     2521 ttcgccgat
//
LOCUS       DASUCJ010001416         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_779254, whole genome shotgun sequence.
ACCESSION   DASUCJ010001416 DASUCJ010000000
VERSION     DASUCJ010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779254"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFU90_16465"
     CDS             complement(<1..394)
                     /locus_tag="VFU90_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5481442.1"
                     /translation="MSKRTYGQYCGFARALELVGERWALMIVRDLLVEPKRFSDLQRG
                     LPGIPTNILTARLKELEQSDIVRRRLLPRPSGAVVYELTETGRGLEAPVHALGAWGAK
                     LLGDPRSHEIVTPDSLVTALRTTFQPAAA"
     gene            543..1010
                     /locus_tag="VFU90_16470"
     CDS             543..1010
                     /locus_tag="VFU90_16470"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5481443.1"
                     /translation="MQKIRPWLWFDTQAEEAANFYVATFPNSRITNLSRYGEEGPGPA
                     GSVMVASFVLDGVEFMALNGGSQAEYGSAFYVECETQEEIDHLWGRLSEGGEPGVCGW
                     LKDRFGVSWNIVPRIFGELMSDDDDEKTGRVMKAMLGMKKLDIAELLRAYEHA"
     gene            complement(987..1970)
                     /locus_tag="VFU90_16475"
     CDS             complement(987..1970)
                     /locus_tag="VFU90_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEU5481444.1"
                     /translation="MEPFALDVVGVGNAIVDVIATTDDRFLAEHGMSKGSMMLVDQAR
                     AEAIYAKMGAALIASGGSAANTIAGIASFGGKTGFIGKVRDDELGHAFRHDITAAGTL
                     FPTPPATEGPATATCLVLVTPDSQRTLNTYLGACANLAAEDLDAGLIRAARFTYLEGY
                     LYDAPSAQNAFHEASVIAHAARRRVALTLSDPFCVKRHRDAFLQLVDRHVDVLFANQF
                     EIGALFETDRRDEIVGRLRASTDLAAVTYGAAGSLIVTPDDVVEIPAAPVAKVVDTTG
                     AGDLYAAGFLFGLTRDMPLSACGRLGSLAAAEIIGHFGARPQSDLRVLVSA"
     gene            2215..>2442
                     /locus_tag="VFU90_16480"
     CDS             2215..>2442
                     /locus_tag="VFU90_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481445.1"
                     /translation="MLLGLPAAAEGTARIQQADGTVNVYQHVKIQLVQKSLTMTTADG
                     KGTMIIYRAACSFQGDVMSCLPTGITLVQSGA"
BASE COUNT          434 a          735 c          841 g          432 t
ORIGIN      
        1 tggcggcggc cggttgaaag gtcgtgcgca gcgccgtgac gagcgagtcg ggcgtgacga
       61 tctcgtgcga acgcgggtcg ccgagcagct tggcgcccca cgcccccagc gcatggaccg
      121 gcgcttcgag tccgcggccg gtttcggtga gttcgtagac gaccgcgccc gacggtctgg
      181 gcagcagccg ccggcggacg atgtcggact gctcgagctc cttgagccgg gcggtcagga
      241 tgttggtcgg gattccgggg aggccgcgtt gcagatcgct aaaccgtttg ggttcgacca
      301 gcagatcgcg gacgatcatc agcgcccagc gctcccccac cagctcgagc gcacgcgcga
      361 acccgcagta ctggccgtag gtacgcttgc tcatcccgca tagtatagag catacttgtt
      421 atattgtcca tttacttgct ttatacaagt aagtttgata tcgttggacg gcgccgggct
      481 gcgatcgtcg ccggccgcgg ggtatacaat cgtcgtgccg atgaccgaag gaggcaatcc
      541 ggatgcagaa gatcagaccg tggctatggt tcgacacgca agccgaggag gcggccaact
      601 tttacgtcgc caccttcccg aactccagga tcaccaacct cagccgctac ggtgaggaag
      661 gccccggacc ggcgggttcg gtgatggtcg cgagcttcgt gctcgacggc gtggagttca
      721 tggcgctcaa cggcggcagc caagccgagt acggttcggc gttctacgtg gaatgcgaga
      781 cgcaagaaga gatcgatcac ctgtggggcc ggctttccga aggcggcgag ccgggcgtgt
      841 gcggctggct caaagaccgc ttcggcgtct cttggaacat cgttccgcgt atcttcggcg
      901 agctgatgtc ggacgacgac gacgaaaaaa ccgggcgcgt catgaaggcg atgctcggga
      961 tgaagaagct cgatatcgcc gagctgttac gcgcttacga gcacgcgtaa atcggactgc
     1021 ggacgcgcgc cgaaatggcc gatgatttcg gccgcggcga ggcttcccaa gcgtccgcat
     1081 gcggacagcg gcatgtcgcg cgtcagcccg aataggaaac cggctgcgta gaggtcgccc
     1141 gcgccggtgg tgtcgaccac cttcgcgacg ggcgccgccg ggatctcgac gacgtcgtcc
     1201 ggcgtaacga tcagcgaccc ggcggcgccg tacgtgaccg ccgctaaatc cgtcgacgcg
     1261 cgcagccggc cgacgatctc gtcgcgacgg tcggtttcga acagcgcgcc gatttcgaac
     1321 tgatttgcga acaacacgtc cacgtggcga tcgaccagct gcaggaacgc gtcgcgatgc
     1381 cgtttgacgc agaacggatc ggagagcgtt agcgcgacgc ggcggcgcgc cgcgtgcgcg
     1441 atgacggaag cttcgtgaaa ggcgttctgc gccgacggtg cgtcgtacag gtagccttcg
     1501 agataggtaa agcgcgccgc acgaatgagg ccggcgtcga gatcctcggc ggcgaggttc
     1561 gcgcacgcgc cgaggtacgt gttgagcgta cgctgcgagt ccggcgtcac caggacgagg
     1621 caggttgcgg tcgccggacc ttcggtcgcc ggcggggtgg gaaagagcgt tccggcggcg
     1681 gtgatgtcgt ggcggaaagc gtgtccgagc tcgtcgtcgc gcaccttgcc gatgaaaccg
     1741 gtctttcccc cgaacgaggc gattccggcg atcgtgttcg ccgccgaccc tcccgacgca
     1801 atcagcgcgg cgcccatttt cgcgtagatg gcttccgcgc gagcctggtc gacgagcatc
     1861 atcgaaccct tcgacatgcc gtgctcggca agaaagcgat cgtcggtcgt tgcgatcacg
     1921 tcgacgatag cattgccgac gccgacgacg tcgagagcga acggttccat cgcggccttg
     1981 cattagtgct tgcgttgccg agttcccggc cgggcggccc tttcgccggg cgggaagggc
     2041 atgaggcgga aacgaagggg cgctatctcg tctgcgccgc tgaaacgtcg cgtgtgggcg
     2101 tgagatatgc cggcaattcc agctcaaaat ggcgcgcgag aaggagtgtc tatgcaacta
     2161 ttcggggcaa cgctacgatt cttcgcgggg gtttgtgcct tcgctgcggg gaccgtgttg
     2221 cttggtttac cggccgcggc ggagggcacc gcgcgcattc agcaggccga cggtacggtc
     2281 aacgtctacc agcacgtcaa gattcagctc gttcagaagt cgctgacgat gacgacggcc
     2341 gacggtaagg gcacgatgat catctatcga gcggcgtgtt cgttccaggg tgacgtcatg
     2401 tcgtgcctgc cgacgggcat cacgctcgtg caatccggcg cc
//
LOCUS       DASUCJ010001417         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_978150, whole genome shotgun sequence.
ACCESSION   DASUCJ010001417 DASUCJ010000000
VERSION     DASUCJ010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978150"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFU90_16485"
     CDS             complement(<1..699)
                     /locus_tag="VFU90_16485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5481446.1"
                     /translation="MIAIALAAAAALLHLATVARYGYFRDELYFIACSKHLAWGYVDQ
                     PPLVALAAWLASPAGYGLAALRALPVAAAILTTYLAVQLARELGGGRFAQIVAGAATL
                     LAPAYLLLGNTLTTTSFEPLSWTLAIYACIRIVRGARLGLWALLGVIVAFGAYGKYSI
                     ALLAIAVAVGLITTPQRTVLVPLRGPLLSAAVALLLLAPNLGWQVAPGWPIVEVLRGD
                     AAHRPGLQNGLALES"
     gene            complement(696..>1268)
                     /locus_tag="VFU90_16490"
     CDS             complement(696..>1268)
                     /locus_tag="VFU90_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481447.1"
                     /translation="LGLATCLILAHFGTAYAFDPAAALNNAPPPIAPFTPDYLTALSI
                     GYYKVSLGVLVVIFQSLLVGGFVLWWVSRIRPCAGGLTLFLLVGSIPPAAAFTNQSAL
                     LAVTVAQALAAGIVGELLIARWDPQPDRPIAFRWFAAAIPMTYIGVYLLGTALSGGLW
                     WDWNVALGAWVWSGVFGFVLSLLPTARRTA"
BASE COUNT          264 a          424 c          431 g          149 t
ORIGIN      
        1 cgattcgagg gccaggccgt tctgcagccc agggcggtgg gcggcgtccc cgcgcagcac
       61 ctcgacgatc ggccagccgg gcgcaacctg ccagccgagg ttcggcgcca gcagcagcaa
      121 cgcgaccgcc gccgagagca acggcccgcg gagcgggacg agcacggtac gctgcggcgt
      181 cgtgatgagt ccgacggcaa cggcgatcgc cagtaacgcg atcgagtatt tcccgtaggc
      241 gccgaacgcc acgattacgc ccagcagcgc ccatagtccg aggcgcgccc cgcgcacgat
      301 ccggatgcag gcgtagatcg cgagcgtcca cgaaagcggc tcgaacgagg tcgtcgttag
      361 ggtattgccg agcagcaggt atgccggcgc gagcagcgtc gccgcgccgg cgacgatttg
      421 agcgaagcgg ccgccgccta gttcgcgcgc cagttgcacg gccaggtagg tcgtgaggat
      481 cgcggccgcg accggaagcg cgcgcagtgc cgccaggccg tatccggccg gtgacgccag
      541 ccaggctgcc agcgcgacga gcggcggctg atcgacgtag ccccaggcga gatgcttcga
      601 acaggcgatg aagtagagtt cgtcgcgaaa ataaccgtaa cgcgcgaccg tcgcgagatg
      661 aagaagcgcc gccgccgcgg ccagcgcgat cgcgatcaag ccgttcttcg agcggtcggc
      721 aacaggctca gaacgaagcc gaaaaccccc gaccacaccc acgctcccaa cgcgacgttc
      781 cagtcccacc acagcccgcc ggaaagcgcc gtgcccaaga ggtagacgcc aatatacgtc
      841 atcggaatcg ccgcggcaaa ccatcgaaag gcgatcggac gatcgggctg cggatcccag
      901 cgtgcgatca gcagctcgcc gacaattccg gccgccagtg cctgcgcgac ggtgaccgcg
      961 agcaacgcgg actggttggt aaacgccgct gcgggcggga tgctgccgac caggagaaac
     1021 agcgtgaggc ctccggcgca cggacgaatc cgcgaaaccc accaaagcac gaagccgccg
     1081 accagcaggc tttgaaaaat gacgacgagc acccccagcg acaccttgta gtatccgatc
     1141 gatagcgccg ttaagtagtc gggcgtgaac ggtgcgatcg gcggcggagc gttattgagc
     1201 gccgctgccg gatcgaaggc gtacgccgtg ccgaagtgcg cgagaatcag gcacgtcgcc
     1261 aggccgag
//
LOCUS       DASUCJ010001418         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1191744, whole genome shotgun sequence.
ACCESSION   DASUCJ010001418 DASUCJ010000000
VERSION     DASUCJ010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191744"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFU90_16495"
     CDS             <1..411
                     /locus_tag="VFU90_16495"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEU5481448.1"
                     /translation="TLGVLAAVEMLADKVPLLDHVLHFLQIALKPAAAAVVVGGAVHA
                     PSHDALVTLMVLGALNALGVHAAVATLRGASTVATGGVANPAVSTVEDAGSIGALFLS
                     FVAPFVAAALALLFTAALATLAVTAYRRFRAART"
     gene            482..892
                     /locus_tag="VFU90_16500"
     CDS             482..892
                     /locus_tag="VFU90_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481449.1"
                     /translation="MEMHLLGRLSLGLVLSAAVAGVSTAPATAAAPVLVKQCFVSQPK
                     PLSKNASGTQIDYIIYGKKNASQITFAVAYRNAAQHFLRTVTDYGSFAPGVEIQHHFD
                     LYNDVTYAGKQVQSCVPIKVKWADSTLWVAPSSH"
BASE COUNT          173 a          372 c          332 g          198 t
ORIGIN      
        1 acgcttggcg tgctcgccgc cgtcgagatg ctcgcggaca aagtcccgct gctcgaccac
       61 gtgctgcatt tcttacagat cgcgctcaag ccggccgccg ccgccgtcgt ggtcggcgga
      121 gcggtgcacg caccgtcgca cgacgcgctg gtaacgctaa tggtcctggg agcgctcaac
      181 gcgctgggcg tgcacgcggc ggttgcgacg ctgcgcggcg cgtcgacggt ggcaaccggc
      241 ggcgtcgcca atccggccgt cagcaccgtt gaagatgccg gttcgatcgg cgcgctcttc
      301 ctttcgttcg tcgcgccgtt cgtcgccgca gcgctcgcgc ttctcttcac ggcggcactt
      361 gcgacgctcg cggttaccgc ctaccgccgt ttccgcgcag cgcgcacgtg aacgggcgga
      421 gccgggagcg gctccgtcga agggaccgct tccaagcttt ccaacactga aaggagcgcg
      481 gttggagatg caccttctcg gacgtctctc actcggactc gttctctccg cggcggttgc
      541 gggcgtttca acggcaccgg cgacggcggc ggctcccgtc ctcgtcaagc agtgcttcgt
      601 ctcacaacca aagccgctca gcaagaacgc cagcggcacg cagatcgact acatcatcta
      661 cggtaagaag aacgcgtcgc aaatcacgtt tgcggttgcc tatcgcaacg ccgcgcagca
      721 cttcctgcgc accgtcaccg actacgggtc gttcgcaccc ggcgtcgaaa ttcagcatca
      781 cttcgacctc tacaacgacg tgacgtacgc cggcaagcaa gtgcagtcgt gcgtccccat
      841 caaggtcaag tgggccgact ccacgttgtg ggtagcaccg tcgtcgcact gacgcgatag
      901 ttttcttccg aagtctcgcg cgagccccgg atgagtctcc ggggctcgcc gcttatccgg
      961 gcgcgtcctt gaagcggtgc ttgccgaggg cgtacaccag gattccgctg ccggccatca
     1021 gcgcggtcag gccgagcacc ttcagcacgg aaagcacctt gttcggatcg tcgtc
//
LOCUS       DASUCJ010001419         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_855565, whole genome shotgun sequence.
ACCESSION   DASUCJ010001419 DASUCJ010000000
VERSION     DASUCJ010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855565"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="lexA"
                     /locus_tag="VFU90_16505"
     CDS             <1..565
                     /gene="lexA"
                     /locus_tag="VFU90_16505"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5481450.1"
                     /translation="TYAEQNGYAPSFEEIARQFNYNSLATVHEHLTNLERKGYIKRAY
                     NESRAIEILPSDAYPRAVELPLLGSVAAGMPIEAIEVNETVSVPESFIGRAGNHFVLR
                     VRGNSMIDEQIRDGDFVVINERHAADNGDTVIALIDNASATVKKFYRERDGRIRLQPA
                     NETMSPIYVHENDVTIQGVVVGVLRRY"
     gene            complement(562..>1042)
                     /locus_tag="VFU90_16510"
     CDS             complement(562..>1042)
                     /locus_tag="VFU90_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481451.1"
                     /translation="ASAGRQVVGVSDPARLELISAALAALGSRHALVVHGEPGMDEMS
                     PVGCTDVHEVRDGKVETRWKFDPAIEAVPSAPHTVAELAGGSPHDNAAMVERIISGRG
                     TGAAATAVMLNAAAAIYVSARAPSFGDALRQAEAALRDGVGAAALERLRAASRRAER"
BASE COUNT          178 a          351 c          353 g          160 t
ORIGIN      
        1 cacgtacgcg gagcagaacg gatacgcgcc gagtttcgag gagattgcgc gacaattcaa
       61 ctataactcg ctggccacgg tccacgagca cctcacgaat ctcgagcgca aggggtacat
      121 caaacgcgcg tacaacgaaa gccgcgcgat cgagattcta ccatcggatg cgtatccgcg
      181 cgcggtcgag ctgccgttgt tgggcagcgt ggccgccggc atgccgatcg aggcgatcga
      241 ggtgaacgag acggtgagtg ttcccgagtc gttcattggg cgcgcgggca atcacttcgt
      301 gctgcgcgtc cgaggcaact cgatgatcga cgagcagatt cgggacggcg atttcgtcgt
      361 gatcaacgag cggcacgcgg ccgacaacgg cgacacggtg attgcgctca tcgacaacgc
      421 gtcggccacg gtcaagaagt tttatcgcga gcgcgacgga cgcattcgcc tgcagccggc
      481 gaacgagacg atgtcgccga tttacgtcca cgagaacgac gtgaccatcc agggcgtcgt
      541 ggtgggtgtg ctcaggcgct attagcgttc ggcgcggcgg ctggcggcgc ggaggcgttc
      601 gagggccgcg gcgcccaccc cgtcgcgcag ggccgcctcc gcctggcgga gcgcgtcccc
      661 aaacgacggc gcccgcgccg agacgtagat cgcggcggcg gcattgagca tcacggccgt
      721 ggccgcggcg cccgttccgc ggccgctgat gatccgctcg accattgccg cgttgtcgtg
      781 cggcgatccg ccggccagct cggcgaccgt gtggggcgcc gacggcacgg cttcgatggc
      841 cggatcgaac ttccagcgcg tttcgacttt cccgtcgcgc acctcgtgca cgtcggtgca
      901 tccgacgggc gacatctcat ccatccccgg ctcgccgtgc acgacgagcg cgtgacggct
      961 gcctaacgca gccagcgccg ccgagatgag ctccagccgc gccggatcgg agacccccac
     1021 cacctggcgc cccgccgatg cc
//
LOCUS       DASUCJ010001420         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_626548, whole genome shotgun sequence.
ACCESSION   DASUCJ010001420 DASUCJ010000000
VERSION     DASUCJ010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626548"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFU90_16515"
     CDS             <1..897
                     /locus_tag="VFU90_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU5481452.1"
                     /translation="SGKRDGADFHVCFQPEFLREGSSIRDYDKPPFTIVGANAEAPRA
                     ALRELFGHLPCEFHSTSVRAAEMVKYSCNNFHALKITFANETARLCEAMKVDPFEVMD
                     LVCKDRQLNISPAYLKPGFAFGGSCLPKDLRATMYFAKTHDVELPVHGAILDSNRRHI
                     DHAIAKVMESGRRRVGFIGLSFKTGTDDLRESPLVLLAEHFIGKGLSLLVYDPEVELS
                     RLLGANRRFIERHLPHIGELIRHEMREVVEASEVLVVGLGGERVASELARLVRDDQLV
                     VDLVRLPAAEKLRARYEGLCWR"
     gene            1169..>1509
                     /locus_tag="VFU90_16520"
     CDS             1169..>1509
                     /locus_tag="VFU90_16520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5481453.1"
                     /translation="MTFDDGYADNATVALPILREAGVHATFFVATSYLDGGRMWNDTV
                     IEAVRAARGPGLDLAAAGLGEHAIATVAERRATIDRLLATLKYAEPERREGLAGAVAS
                     VAGVALPDDLM"
BASE COUNT          240 a          488 c          511 g          270 t
ORIGIN      
        1 tcgggcaagc gcgacggggc agacttccat gtctgctttc aacccgagtt tctccgcgaa
       61 ggaagctcga tccgcgacta cgacaagccc ccgttcacga tcgtaggtgc gaacgccgag
      121 gctccgcgcg ctgcgctgcg cgagctcttc ggtcatctgc cctgcgagtt ccattcgacg
      181 tcggtgcgcg cggccgaaat ggtgaagtac agttgcaaca acttccacgc gctcaagatc
      241 accttcgcga acgaaacggc acgactatgc gaggcgatga aggtcgatcc cttcgaggtg
      301 atggatctcg tctgcaagga ccggcagctc aacatctcgc cggcctatct caagccgggc
      361 ttcgcgttcg gcgggtcgtg cctgccgaag gatctccgcg cgacgatgta cttcgccaag
      421 acgcatgacg tcgagctgcc ggtgcacggc gcgattctcg actccaaccg gcggcatatc
      481 gaccacgcga tcgccaaggt gatggagagc ggcaggcggc gcgtgggatt catcggcctt
      541 tcgttcaaga ccggcaccga cgacctgcgc gagagcccgc tcgtcctcct cgccgagcac
      601 ttcatcggca agggactctc gctgctggtc tacgatccgg aggtcgagct ctcgcgcctg
      661 ctgggagcga accgccggtt catcgagcgg catcttccgc acatcggtga gctgatccgg
      721 catgagatgc gagaagtcgt cgaggcatcg gaggtgctcg ttgtcgggct tggcggcgaa
      781 cgcgtcgctt cggagcttgc acgtctggtc cgcgacgatc agctcgtcgt cgatctggtt
      841 cggcttcccg cagcggagaa gctccgggcc cgatacgagg ggctttgctg gcggtgaata
      901 ccgacgaaat ggcggagcgg ccgatgaccg cgcggcgccg caaaccgtcg cgaccgccat
      961 cggggattgc ggacgggctc gtgcgcgcct ggttccggcg gctgtctccg gcgggttcgc
     1021 gggcgcggct aggcatcgtc atgtttcatc gcgtgcgcac gtcgccggat gaattgtttc
     1081 ccgcagagat cgatgccgcg ggattccgcg cccgcatcga ctggctgcgg cgctcgcgcg
     1141 tggcggcctc cccgagcgcg cattctgcgt gacgttcgac gacggctatg cggacaacgc
     1201 aaccgtagcg ctgccgatcc tgcgcgaagc cggggtgcac gcgacgttct tcgtggcgac
     1261 gtcgtatctc gacggtggac ggatgtggaa cgatacggtg atcgaggccg tgcgagcggc
     1321 gcgcgggcca ggtctcgatc tcgccgctgc cggcctcggt gagcacgcaa tcgccacggt
     1381 cgccgagcgg cgcgcaacga tcgaccggct gctggccacc ctcaagtacg ccgagcccga
     1441 gcgccgtgaa ggtttggctg gcgccgttgc atcggtcgcc ggcgtggcgc ttcccgacga
     1501 cctgatgat
//
LOCUS       DASUCJ010001421         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_397358, whole genome shotgun sequence.
ACCESSION   DASUCJ010001421 DASUCJ010000000
VERSION     DASUCJ010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397358"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VFU90_16525"
     CDS             <1..1535
                     /locus_tag="VFU90_16525"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEU5481454.1"
                     /translation="HRLCVAVFFLLLPLSVLPARAAETLSPATISITDLFRRVRKAEG
                     QPTQSAYHIVMRSVVEGETTVEDTYVDPRGYKTVETAAGFTSSWGALGGQGWYQNSNG
                     FVQRVSGVYAQNDPIEHAVREIGSSAGSAQLLGMTAGSPAMYVVELSPKPGLSEKRYY
                     DADTYLLRRIELTDYDGRTRISEYDDYRSVLGRMVPFVRTYQADFSKESRRYDVITYE
                     AVRPGVADLSIPASKTLFDLRGRDSVTIPADFTDDGIIVRMNVGQRGLDMLLDSGSSS
                     TVVNAAVASQLGLAVHNKRLESFGGIFSLSDARVDDISVGPLQAASAAINIAPVDDDV
                     APTQRIVGLLGCDFIASGALEVDFTHKSLTLYAHPPADLVASGWTELPITLDDCVPLT
                     KAAFSGLAGEFLIDLGAYDTVLYDHYFAQFKGSSTPKGGDPLINEGSFIGGDEVRFQT
                     YSMRTLSIGNLLFADAVVDVPLTKKVQERDYDGLLGRTTLTSFDILFDYAHQRVYLKP
                     RI"
     gene            1743..2819
                     /locus_tag="VFU90_16530"
     CDS             1743..2819
                     /locus_tag="VFU90_16530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5481455.1"
                     /translation="MRSAISAAVERDRAHYGGNTPIPATVIGLWDGKGHSFVKTFGYS
                     NLATKTPVTTADHFRIGSNTKTFVVAVLLQLVTEKKLSLDDPLSRFNLGVKIPNAENI
                     TVRELCDMRSGLYEAYDTPQFVQLDWKVPAGFNQRMLVEWAVQHKPYFAPNQGYRYSN
                     TNYILLGLIIESLTKDSVGDQIRRRLIEPYHLAQTSFPQTEAMPEPWVRGYALAKSGN
                     WEDVSNTVPVAFMWSAGEMISDVGDIRRWIQLFTTGRAGGSATYKDLVNCIPFLGNTS
                     FGLGITCSAGWYGYTGALPGYNTADYYSPSTGMTIVAWINYQAAQPIEGVASVMVRDI
                     ARIVTPGHVPFVYTQAELQQLTGH"
     gene            2874..>3273
                     /locus_tag="VFU90_16535"
     CDS             2874..>3273
                     /locus_tag="VFU90_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481456.1"
                     /translation="MVKAFGISLLLMAALAGFPPPAAAQTAPLTGEMASFTYLIGGAW
                     SCATTVPATSATNAYTERATVTFELAPGNTIHEHGVSDRYAFDRYFGYDAGQRNYFST
                     NADNRGNFGFLSSSDGVRYTGREHVGANQEY"
BASE COUNT          637 a         1079 c          960 g          597 t
ORIGIN      
        1 atcatcggct ctgcgtcgcc gtctttttcc tattgcttcc gctctcggtg ttgccggcaa
       61 gggcggccga gacgctttcg ccggctacga tatcgattac cgatctattt cgccgggtga
      121 ggaaggccga gggccaaccg acgcaaagcg cctaccacat cgttatgcgt agcgtcgtcg
      181 agggcgaaac gaccgtcgag gacacctacg tcgacccgcg gggatacaaa acggtcgaaa
      241 cggcggcggg gtttacgtcc tcgtggggcg cgctaggagg ccagggctgg tatcagaatt
      301 cgaatggatt cgtccagcgc gtgagcggcg tttacgcgca gaacgatccg atcgaacacg
      361 ccgtccgcga aatcggcagc agcgccggct ccgcccagct attgggcatg acggccggct
      421 caccggctat gtacgtcgtc gagctgagcc caaaacccgg attgagcgag aagcgttact
      481 acgacgccga tacctatctg ctgcgccgga tcgagcttac ggactatgac ggccgtacgc
      541 gcatctccga atacgacgac taccgaagcg ttctgggcag aatggtgccg ttcgtccgga
      601 cctaccaagc cgacttttcc aaagagagcc gccgttacga cgtcatcacc tacgaagccg
      661 tccgtccggg cgtggccgat ctcagcatcc ctgcgtccaa gaccttgttc gacctgcggg
      721 ggcgcgactc ggtgaccatt ccggcggatt tcaccgacga cggaatcatc gtgcgaatga
      781 acgtcggcca gcgcggactg gatatgctgc tcgattccgg ttcgtcatcg acggtcgtca
      841 acgccgcggt agcttcgcag ctcgggctcg cggttcacaa caaacgcctc gaaagcttcg
      901 gcgggatctt ttccttgtcc gatgcccggg tcgacgatat ctcggtcgga ccgcttcaag
      961 cggcatcggc cgccatcaac atcgctccgg tcgacgacga cgtcgccccg acccagcgca
     1021 tcgtgggctt actcggatgc gatttcatcg ctagcggagc gctcgaagta gacttcacgc
     1081 acaagtcgct cacgctgtac gcgcacccgc ccgccgacct ggtagcgagt ggctggacgg
     1141 agctaccgat aacgctcgac gactgcgttc ccttgacgaa ggccgccttc agcgggttgg
     1201 cgggagagtt cctgatcgac ctcggcgcct acgacacggt gctgtacgac cactatttcg
     1261 cgcagttcaa gggaagcagc acgcccaagg gcggcgaccc gctgatcaac gaaggaagct
     1321 ttatcggtgg cgacgaggtc cggtttcaaa cctactcgat gcgaacgcta agcattggga
     1381 accttctctt tgccgacgcc gtggtcgacg ttccccttac gaaaaaggtt caggagagag
     1441 actacgacgg tctgctcggc cgaaccacgc tcaccagctt cgacatcctc ttcgactacg
     1501 cacaccagcg cgtctatttg aagccgcgaa tctgaacgac cggcggcatc gtgcacgagg
     1561 ggacggaagg gccgatgctg cggcgtccga atgtgccatg agctacgttc tattttgaag
     1621 gagctgccgt gcaccgtcac gtttttacat tcgccgcgcc cgttttcgga tgcgcgcccc
     1681 tcgcattcaa cgtacccgtc gctgcgccgg cagcccaact cccgtcgctg acgagcccgt
     1741 cggtgcgatc ggcgatttcg gcggcggtag agcgcgatcg cgcccattat ggcggaaaca
     1801 cgccgatccc cgctaccgtc atcggccttt gggacggcaa ggggcacagc ttcgtcaaga
     1861 cgttcggcta ttcaaacctc gcaaccaaga cgccggtgac gacggccgac catttccgca
     1921 tcggcagcaa caccaagacg ttcgtcgtgg ccgtcctgct ccagctcgta accgagaaga
     1981 aattatcgct cgacgatccg ctgagccgct tcaacctcgg cgtcaagatt ccgaacgcgg
     2041 aaaacatcac cgttcgcgaa ctctgcgaca tgcgcagcgg cctctacgaa gcctacgaca
     2101 cgccgcagtt cgtgcagctc gattggaagg tgcccgccgg tttcaaccag cgcatgctcg
     2161 tcgagtgggc cgtgcagcac aagccgtact tcgcgccaaa ccaaggctac cggtacagca
     2221 acaccaacta catcctgctc gggctcatca tcgaatcgtt gaccaaggat agcgtgggcg
     2281 atcagattcg ccgccggctg atcgagccgt accatctcgc gcagacgtcc tttccgcaaa
     2341 ccgaggcgat gcccgagccg tgggtgcgcg gttacgcgct ggcaaagagc ggcaactggg
     2401 aagacgtcag caacaccgtg ccggtcgcgt tcatgtggtc ggccggcgaa atgatctccg
     2461 acgtgggcga tatccgccgc tggatccagc tcttcaccac cggtcgggcc ggcggaagcg
     2521 cgacctacaa agacctcgtc aactgcatcc ccttcctggg aaacacctcg ttcggtctcg
     2581 gcatcacctg cagcgccggc tggtacggct acaccggcgc gctgcccggc tacaacaccg
     2641 ccgactacta ttcgccgtcg accggtatga cgatcgtcgc ctggatcaac tatcaagccg
     2701 cgcagcccat cgaaggcgtt gccagcgtca tggtgcgcga catcgcacgc atcgttacgc
     2761 cgggccacgt accgttcgtc tacacccagg ccgaactgca gcagttgacc gggcactagc
     2821 aggccgtccg tgcgccgacc agggcggcgg ccgttcaaaa cgaagacagc ggcatggtga
     2881 aagcgttcgg gatttcgttg cttctaatgg ccgcgctggc cggctttccg ccgccggctg
     2941 ccgcccagac cgccccgcta acgggcgaga tggcgtcctt tacgtatttg atcggcggcg
     3001 cgtggagctg cgcgaccacc gttccggcga cctcggcaac caatgcctat accgaacgcg
     3061 cgaccgtaac gtttgagctc gccccgggca atacgatcca cgagcacggc gtctccgacc
     3121 gctacgcgtt cgaccgttat ttcggctacg acgccggtca gcgcaattat ttcagcacca
     3181 atgccgacaa tcgcggcaac ttcgggttcc tgtcgtcgtc cgacggcgtc cgctacaccg
     3241 ggcgcgagca cgtcggcgcc aatcaagaat acc
//
LOCUS       DASUCJ010001422         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_1161464, whole genome shotgun sequence.
ACCESSION   DASUCJ010001422 DASUCJ010000000
VERSION     DASUCJ010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161464"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="rlmD"
                     /locus_tag="VFU90_16540"
     CDS             complement(<1..992)
                     /gene="rlmD"
                     /locus_tag="VFU90_16540"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU5481457.1"
                     /translation="MTTKETALKTSGKPALPRAGDELDVAFTDLLANGQAVGRCDGVV
                     VFCAGPLPGERARVAIALVKPKYAVAELRELLTQSPERVTPFCPVFGECGGCQVQHLR
                     YDAQLQWKREVLRGALERIGGFRGADVRETIGMAVPRHYRNKMSLVASQRDGATAFGF
                     YRQRSHEVVPIEACPIVTEQLSGYVGRLTSLAGDAGARGSAAEFANAFAEARHVVARD
                     ARASGEAVVTVTTARESAAVLRSGAALMAALPGVAGVTNSFELSSENAILGRKHRLAA
                     GRAEIEESIGGVRYRVSAGSFFQVNVEIVGRIFEFLAPRLQVPRAIVDLYCGAGT"
BASE COUNT          180 a          398 c          284 g          152 t
ORIGIN      
        1 gtccccgcgc cgcaatacaa atctacgatc gcacgcggaa cttgcaatcg cggtgccaga
       61 aattcgaaaa tccgtccgac gatttcgacg ttcacttgaa agaacgaccc cgccgacacg
      121 cgatagcgca cgccgccgat cgactcctcg atctccgcac gccccgccgc caaacgatgc
      181 ttacgcccca gaatcgcatt ttcactcgac aactcgaacg aattcgtcac tcccgccacg
      241 cccggcagcg ccgccatcaa cgccgcgccc gatcgaagca ccgccgccga ctcgcgcgcc
      301 gtcgtcaccg tcaccaccgc ctcgcccgac gcgcgcgcgt cgcgcgccac cacgtgccgc
      361 gcctccgcaa acgcgttcgc aaactctgcc gccgaaccgc gcgcaccggc gtcgccggcc
      421 agcgacgtca gccgtcctac gtacccgctg agctgctcgg tgacgatcgg gcacgcctcg
      481 atcggcacga cctcgtgcga acgctgccgg taaaacccga acgccgtcgc accgtcgcgc
      541 tgcgacgcca ccagcgacat tttgttgcgg tagtgccgtg gaacggccat gccgatcgtt
      601 tcgcgcacgt cggccccgcg aaaaccgccg atccgttcga gcgcgccgcg cagcacctcg
      661 cgcttccact gcagctgcgc gtcgtagcgc aagtgctgca cttggcagcc gccgcactcg
      721 ccgaacaccg ggcaaaacgg cgtcacccgc tccggcgact gcgtcagcag ctcgcgcagt
      781 tccgccaccg catacttcgg cttgacgagc gcgatcgcga cgcgtgcgcg ctcgcccggc
      841 aacggccccg cgcaaaacac gacgacgccg tcgcatcgtc ctaccgcctg accgttggca
      901 agcaagtcgg tgaatgcgac gtcgagctcg tcgccggcgc gcggcagcgc cggtttgccg
      961 gaagtcttaa gcgctgtttc tttggtcgtc aagcgttttg agcagcgagt tcgc
//
LOCUS       DASUCJ010001423         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_504334, whole genome shotgun sequence.
ACCESSION   DASUCJ010001423 DASUCJ010000000
VERSION     DASUCJ010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504334"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFU90_16545"
     CDS             <1..237
                     /locus_tag="VFU90_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481458.1"
                     /translation="EARKTNAEFIDDVADDVPKRLLEIARAKPETTMAVSGTKRPSRW
                     LKRRSFARRLLDAGARELLVLMPPGKIAEAAPES"
     gene            complement(241..816)
                     /gene="kdpC"
                     /locus_tag="VFU90_16550"
     CDS             complement(241..816)
                     /gene="kdpC"
                     /locus_tag="VFU90_16550"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEU5481459.1"
                     /translation="MLRHLGTALRVTILSIVTLGLIYPIAMTGVAQALFPRQANGSLV
                     TVNGKVVGSWIVGQLWTKPQYFQGRPSAAGKGYDPTSTGGTNLGPTSAKLIKATKQTI
                     AQLEKDNPGASGPPPIDLVTSSASGIDPDISPEAAYWEAPRVAKARHLNLTAVRALVA
                     AHVAGRQFGFLGEPHVNVLELNLALDGLHPQ"
     gene            complement(828..>1244)
                     /locus_tag="VFU90_16555"
     CDS             complement(828..>1244)
                     /locus_tag="VFU90_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEU5481460.1"
                     /translation="AGNMVDLDSDPTKLIEVVEIGKQLLMTRGALTTFSIANDVAKYF
                     AILPAMFAIAYKPMELLNVMRLTTPQSAILSAVIFNALIIVALIPLALRGVKYTPKGA
                     NAVLFENVMLYGVGGIVVPFVGIKLIDVILAALHLT"
BASE COUNT          227 a          386 c          408 g          223 t
ORIGIN      
        1 gaagcgcgca agactaatgc cgagttcatt gacgacgtcg ccgacgacgt acccaagcgc
       61 ctgctcgaaa tcgcgcgcgc gaagccggaa acgacgatgg cggtctccgg gacgaagcgc
      121 ccgtctcgct ggctcaagcg ccggtcgttc gcgcggcgac tgctcgatgc cggcgcccgc
      181 gagctgctcg tgctgatgcc gccggggaaa atcgccgagg cagctcccga gagctagacg
      241 ctactgcgga tgcagcccgt cgagcgcgag gttcaactcc agcacgttga cgtgcggctc
      301 gccgaggaat ccgaactgac ggccggcaac gtgcgcggcc acgagcgcgc gaacggcggt
      361 gaggttcagg tgacgcgcct tggccacgcg cggcgcctcc cagtacgcgg cttccggcga
      421 gatgtccggg tcgatgccgc tggcgctcga cgtgacgaga tcgatcggcg gcggcccgct
      481 cgcgccggga ttgtcctttt cgagctgcgc gatcgtctgt ttggtggctt tgatcagctt
      541 ggcggacgtc ggcccgagat tcgttccacc ggtcgacgtc ggatcgtaac ccttacccgc
      601 ggccgaggga cggccttgga aatattgcgg tttcgtccac agctgtccga cgatccacga
      661 cccgaccact ttcccgttta ccgtaacgag cgatccgttg gcctgtcgcg gaaagagcgc
      721 ctgagctacg cccgtcatgg cgatcggata gatcaagccg agcgtcacga tgctgaggat
      781 cgtgacgcga agcgcggtgc ctaaatgtcg cagcatgact cgattcctta cgtaaggtgc
      841 agcgccgcga ggatcacgtc gatcagcttg atgccgacga acggaaccac gatgccgccg
      901 acgccgtaga gcatcacgtt ctcgaagagc acggcgttgg cacccttcgg cgtgtatttc
      961 acccctcgca gcgcgagcgg gatcaacgcg acgatgatca gcgcgttgaa tatgaccgcc
     1021 gacagaatgg cgctctgggg ggtggtgaga cgcatgacgt tgagcagttc catcggcttg
     1081 taggctatcg cgaacatcgc aggcagaatg gcgaaatact tcgctacgtc gttggcgatc
     1141 gagaaggtcg tgagcgcccc gcgcgtcatc agcagctgct ttccgatctc gacgacctcg
     1201 atgagtttgg tcggatccga gtcgaggtcg accatgttcc cggc
//
LOCUS       DASUCJ010001424         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Tumulicola sp. isolate SMAG_U2546
            k141_550148, whole genome shotgun sequence.
ACCESSION   DASUCJ010001424 DASUCJ010000000
VERSION     DASUCJ010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961001
            Sequence Read Archive: SRR25158444
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Tumulicola sp. (soil metagenome)
  ORGANISM  Candidatus Tumulicola sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Tumulicola.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:32:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,294
            Genes (coding)                    :: 3,288
            CDSs (with protein)               :: 3,288
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Candidatus Tumulicola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550148"
                     /isolate="SMAG_U2546"
                     /isolation_source="soil"
                     /db_xref="taxon:3069698"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.73972222 N 118.0672222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFU90_16560"
     CDS             <1..813
                     /locus_tag="VFU90_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEU5481461.1"
                     /translation="NAFGVMYGGALAFLADATILLSAATTVPTGTAFNTIDLKLYFLR
                     PVVPADGVLVARGKVVHRGCTIAVVACEITGPDGALVAQATGSVLILPGRHWERPVRV
                     ADEMTADTTRVLTSLLFVDLVDSTQRAAQIGDARWRTVLADYHALVREQIRRFRGREI
                     DTAGDGFFIAFDSAARAIQCAAAVRDGVRRLDLDVRCGIHAGECEESAGKLVGIAVHI
                     GARVGAAAQPGEILVSSTVRDIVVGSGIAFEDRGEHALKGIAGSWQLYAAST"
     gene            complement(942..>1145)
                     /locus_tag="VFU90_16565"
     CDS             complement(942..>1145)
                     /locus_tag="VFU90_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5481462.1"
                     /translation="SVTCSQAKPWLVKILADRGKPGAKAVIKNAPKGFHCSASNDAKG
                     RPFAGGCYTGTLAFPKNGFQWLG"
BASE COUNT          170 a          396 c          375 g          204 t
ORIGIN      
        1 aacgcgttcg gcgtcatgta tggcggtgcg ctcgcgttcc tcgccgatgc cacgatcctc
       61 ctctcggccg cgacgacggt gccgacggga acggcgttca acacgatcga tctcaagctc
      121 tacttcctcc gtccggtggt tcccgccgac ggcgtacttg tcgcgcgcgg gaaggtcgtc
      181 caccgtgggt gtacgatcgc ggtcgtcgcc tgcgagatca cgggtccgga cggcgccctc
      241 gtcgcgcagg cgaccgggtc ggtgctcatc ctccccggcc gccactggga gcgcccggtt
      301 cgggtcgcgg acgagatgac cgccgacact acgcgcgtgc tcacgtcgct gctcttcgtc
      361 gaccttgtcg actcgacgca gcgagcagca cagatcggtg atgcacgctg gcgtacggtg
      421 ctcgccgatt atcacgcgct tgtgcgcgag cagatccgcc gcttccgtgg ccgtgagatc
      481 gacacggccg gcgacgggtt cttcatcgcg ttcgacagtg cggcgcgcgc catacagtgc
      541 gcggccgccg tgcgtgacgg cgtgcgccgg ctcgacctcg acgtccgctg cgggatccac
      601 gcaggggaat gcgaggagag cgccggaaag ctcgtcggca tcgcggttca catcggcgcg
      661 cgcgtcggcg ctgctgcgca gccaggcgag atcctcgtct cgagcaccgt tcgcgacatc
      721 gtcgtcggct ccggaatcgc gttcgaggac cgcggcgagc acgcgctgaa gggcatcgcc
      781 ggcagctggc agctgtacgc cgcgtcgacc tagccggatc ccgcccgcac acgccccatt
      841 tgcaaaagca ccgggtcagg ggatctctcc acataccgtc ctcgctatga gaaagccccg
      901 ctcgtgcggg gctctcgcgt tccgggtgct ggtagctccg ctcagccgag ccactggaat
      961 ccattcttcg ggaacgcgag cgtaccggtg tagcagccac cagcaaacgg tctgcctttc
     1021 gcgtcattcg acgcggagca atgaaagccc tttggggcgt tcttgatgac agccttcgcg
     1081 cccggctttc cgcgatcggc aaggatcttc accagccacg gctttgcctg gctgcaggtg
     1141 acact
//
